Sample records for increasing storage temperature

  1. Effects of Storage Duration and Temperature on the Chemical Composition, Microorganism Density, and In vitro Rumen Fermentation of Wet Brewers Grains

    PubMed Central

    Wang, B.; Luo, Y.; Myung, K. H.; Liu, J. X.

    2014-01-01

    This study aimed to investigate the effects of storage duration and temperature on the characteristics of wet brewers grains (WBG) as feeds for ruminant animals. Four storage temperatures (5°C, 15°C, 25°C, and 35°C) and four durations (0, 1, 2, and 3 d) were arranged in a 4×4 factorial design. Surface spoilage, chemical composition and microorganism density were analyzed. An in vitro gas test was also conducted to determine the pH, ammonia-nitrogen and volatile fatty acid (VFA) concentrations after 24 h incubation. Surface spoilage was apparent at higher temperatures such as 25°C and 35°C. Nutrients contents decreased concomitantly with prolonged storage times (p<0.01) and increasing temperatures (p<0.01). The amount of yeast and mold increased (p<0.05) with increasing storage times and temperatures. As storage temperature increased, gas production, in vitro disappearance of organic matter, pH, ammonia nitrogen and total VFA from the WBG in the rumen decreased (p<0.01). Our results indicate that lower storage temperature promotes longer beneficial use period. However, when storage temperature exceeds 35°C, WBG should be used within a day to prevent impairment of rumen fermentation in the subtropics such as Southeast China, where the temperature is typically above 35°C during summer. PMID:25050021

  2. Effect of Frozen Storage Temperature on the Quality of Premium Ice Cream.

    PubMed

    Park, Sung Hee; Jo, Yeon-Ji; Chun, Ji-Yeon; Hong, Geun-Pyo; Davaatseren, Munkhtugs; Choi, Mi-Jung

    2015-01-01

    The market sales of premium ice cream have paralleled the growth in consumer desire for rich flavor and taste. Storage temperature is a major consideration in preserving the quality attributes of premium ice cream products for both the manufacturer and retailers during prolonged storage. We investigated the effect of storage temperature (-18℃, -30℃, -50℃, and -70℃) and storage times, up to 52 wk, on the quality attributes of premium ice cream. Quality attributes tested included ice crystal size, air cell size, melting resistance, and color. Ice crystal size increased from 40.3 μm to 100.1 μm after 52 wk of storage at -18℃. When ice cream samples were stored at -50℃ or -70℃, ice crystal size slightly increased from 40.3 μm to 57-58 μm. Initial air cell size increased from 37.1 μm to 87.7 μm after storage at -18℃ for 52 wk. However, for storage temperatures of -50℃ and -70℃, air cell size increased only slightly from 37.1 μm to 46-47 μm. Low storage temperature (-50℃ and -70℃) resulted in better melt resistance and minimized color changes in comparison to high temperature storage (-18℃ and -30℃). In our study, quality changes in premium ice cream were gradually minimized according to decrease in storage temperature up to-50℃. No significant beneficial effect of -70℃ storage was found in quality attributes. In the scope of our experiment, we recommend a storage temperature of -50℃ to preserve the quality attributes of premium ice cream.

  3. Effect of Frozen Storage Temperature on the Quality of Premium Ice Cream

    PubMed Central

    Park, Sung Hee; Jo, Yeon-Ji; Chun, Ji-Yeon; Hong, Geun-Pyo

    2015-01-01

    The market sales of premium ice cream have paralleled the growth in consumer desire for rich flavor and taste. Storage temperature is a major consideration in preserving the quality attributes of premium ice cream products for both the manufacturer and retailers during prolonged storage. We investigated the effect of storage temperature (−18℃, −30℃, −50℃, and −70℃) and storage times, up to 52 wk, on the quality attributes of premium ice cream. Quality attributes tested included ice crystal size, air cell size, melting resistance, and color. Ice crystal size increased from 40.3 μm to 100.1 μm after 52 wk of storage at −18℃. When ice cream samples were stored at −50℃ or −70℃, ice crystal size slightly increased from 40.3 μm to 57-58 μm. Initial air cell size increased from 37.1 μm to 87.7 μm after storage at −18℃ for 52 wk. However, for storage temperatures of −50℃ and −70℃, air cell size increased only slightly from 37.1 μm to 46-47 μm. Low storage temperature (−50℃ and −70℃) resulted in better melt resistance and minimized color changes in comparison to high temperature storage (−18℃ and −30℃). In our study, quality changes in premium ice cream were gradually minimized according to decrease in storage temperature up to−50℃. No significant beneficial effect of −70℃ storage was found in quality attributes. In the scope of our experiment, we recommend a storage temperature of −50℃ to preserve the quality attributes of premium ice cream. PMID:26877639

  4. DOE Office of Scientific and Technical Information (OSTI.GOV)

    Kuang, Xingya; Shankar, T.J.; Bi, X.T.

    Wood pellets emit CO, CO2, CH4 and other volatiles during storage. Increased concentration of these gases in a sealed storage causes depletion of concentration of oxygen. The storage environment becomes toxic to those who operate in and around these storages. The objective of this study was to investigate the effects of temperature, moisture and storage headspace on emissions from wood pellets in an enclosed space. Twelve 10-liter plastic containers were used to study the effects of headspace ratio (25%, 50%, and 75% of container volume) and temperatures (10-50oC). Another eight containers were set in uncontrolled storage relative humidity and temperature.more » Concentrations of CO2, CO and CH4 were measured by a gas chromatography (GC). The results showed that emissions of CO2, CO and CH4 from stored wood pellets are most sensitive to storage temperature. Higher peak emission factors are associated with higher temperatures. Increased headspace volume ratio increases peak off-gas emissions because of the availability of oxygen for pellet decomposition. Increased relative humidity in the enclosed container increases the rate of off-gas emissions of CO2, CO and CH4 and oxygen depletion.« less

  5. Canopy storage capacity and wettability of leaves and needles: The effect of water temperature changes

    NASA Astrophysics Data System (ADS)

    Klamerus-Iwan, Anna; Błońska, Ewa

    2018-04-01

    The canopy storage capacity (S) is a major component of the surface water balance. We analysed the relationship between the tree canopy water storage capacity and leaf wettability under changing simulated rainfall temperature. We estimated the effect of the rain temperature change on the canopy storage capacity and contact angle of leave and needle surfaces based on two scenarios. Six dominant forest trees were analysed: English oak (Quercus roburL.), common beech (Fagus sylvatica L.), small-leaved lime (Tilia cordata Mill), silver fir (Abies alba), Scots pine (Pinus sylvestris L.),and Norway spruce (Picea abies L.). Twigs of these species were collected from Krynica Zdrój, that is, the Experimental Forestry unit of the University of Agriculture in Cracow (southern Poland). Experimental analyses (simulations of precipitation) were performed in a laboratory under controlled conditions. The canopy storage capacity and leaf wettability classification were determined at 12 water temperatures and a practical calculator to compute changes of S and contact angles of droplets was developed. Among all species, an increase of the rainfall temperature by 0.7 °C decreases the contact angle between leave and needle surfaces by 2.41° and increases the canopy storage capacity by 0.74 g g-1; an increase of the rain temperature by 2.7 °C decreases the contact angle by 9.29° and increases the canopy storage capacity by 2.85 g g-1. A decreased contact angle between a water droplet and leaf surface indicates increased wettability. Thus, our results show that an increased temperature increases the leaf wettability in all examined species. The comparison of different species implies that the water temperature has the strongest effect on spruce and the weakest effect on oak. These data indicate that the rainfall temperature influences the canopy storage capacity.

  6. [Impact of storage conditions and time on herb of Lonicera macranthoides].

    PubMed

    Ma, Peng; Li, Long-Yun; Zhang, Ying

    2014-03-01

    To study the effect of different storage conditions and storage time on herb quality of Lonicera macranthoides, different packaging materials including vacuum plastic bags, plastic bags, woven bags, sealed with endometrial bags, paper bags, sack bags were selected for the study under different storage conditions including room temperature, 5 degrees C refrigerator, low temperature of - 20 degrees C refrigerator and desiccator. Twenty-four batches of samples were used for the study, and active ingredients were determined. The experimental results showed that the ingredients in each storage group changed with the storage time, storage conditions (storage environment, packaging). Under the same storage time, the storage environment (temperature, humidity) had effect on the stability of herb quality. Low temperature had less effect on herb quality. The effect of packaging on herb quality was as following: plastic vacuum packaging > woven with endometrial sealed packaging > plastic bag > woven bag > sack bags > paper bags. Under the same storage conditions, with the increase of storage time, caffeic acid content increased slowly, and other five ingredients content decreased gradually. Storage time affected significantly on the intrinsic quality (chemical composition) and appearance of herb. It is suggested that low temperature (5 degrees C), dark and sealed storage are suitable for storage of L. macranthoides herb, the storage time should be not more than 24 months.

  7. Relationship between gamma-glutamyl transpeptidase activity and garlic greening, as controlled by temperature.

    PubMed

    Li, Lei; Hu, Dan; Jiang, Ying; Chen, Fang; Hu, Xiaosong; Zhao, Guanghua

    2008-02-13

    It was established that storage at low temperature (less than 10 degrees C) was required for garlic greening occurring either during processing or in the course of "Laba" garlic preparation while storage at high temperature (higher than 20 degrees C) inhibited its occurrence. However, the reason for this observation is unclear. To obtain insights into a tie connected between storage temperature and garlic greening, it was detected if the gamma-glutamyl transpeptidase (GGT) activity correlated with garlic greening because the activity of this enzyme is very sensitive to storage temperature. Results showed that garlic puree (which was prepared from fresh garlic) turned green upon addition of GGT but the color of garlic puree remained unchanged when either water or heat-treated GGT (which has no activity due to heat treatment) was used, a result giving a positive answer to the above proposal. Subsequently, to further clarify the relationship between the GGT activity and garlic greening, the GGT activity, the degree of garlic greening, and the concentration of total thiosulfinates in garlic bulbs were determined respectively after the garlic bulbs had been stored at 4 degrees C for up to 59 days followed by storage at 35 degrees C for up to 22 days. It was found that cold storage facilitated the GGT activity whereas warm storage inhibited the activity of this enzyme, just like the effect of storage temperature on greening, indicating that the increase of GGT activity could be a direct factor resulting in garlic greening. Consistent with this conclusion, the concentration of total thiosulfinates (the color developers) in garlic purees likewise exhibited a reversible change by moving garlic bulbs from one low storage temperature to a higher one; namely, it increased with increasing storage time during storage at 4 degrees C while decreasing as storage time increased during storage at 35 degrees C. The present study provided direct evidence that the GGT is involved in garlic greening.

  8. Storage length, storage temperature, and lean formulation influence the shelf-life and stability of traditionally packaged ground beef.

    PubMed

    Martin, J N; Brooks, J C; Brooks, T A; Legako, J F; Starkey, J D; Jackson, S P; Miller, M F

    2013-11-01

    The effect of storage length and temperature on the shelf life of three ground beef formulations (lean:fat: 73:27, 81:19 and 91:9) was investigated. Coarsely ground beef was stored at -1.7 or 2.3°C for up to 28d. Traditional overwrap packages were produced every 7d prior to retail display for 24h. Lipid oxidation (TBARS), subjective color, instrumental color, and aerobic bacteria were evaluated after 0 and 24h of display. Formulation influenced initial L* and subjective color values (P<0.05). Storage temperature did not affect initial color, but product stored at 2.3°C was more discolored after 24h (P<0.05). Aerobic bacteria increased as storage d and temperature increased (P<0.05). Initial TBARS increased through d 21, but were lower after 28d. Overall, initial characteristics depended on formulation; however, ground beef shelf-life and stability were largely influenced by storage length and storage temperature. Copyright © 2013 Elsevier Ltd. All rights reserved.

  9. Effect of storage temperature on quality of light and full-fat ice cream.

    PubMed

    Buyck, J R; Baer, R J; Choi, J

    2011-05-01

    Ice cream quality is dependent on many factors including storage temperature. Currently, the industry standard for ice cream storage is -28.9 °C. Ice cream production costs may be decreased by increasing the temperature of the storage freezer, thus lowering energy costs. The first objective of this research was to evaluate the effect of 4 storage temperatures on the quality of commercial vanilla-flavored light and full-fat ice cream. Storage temperatures used were -45.6, -26.1, and -23.3 °C for the 3 treatments and -28.9 °C as the control or industry standard. Ice crystal sizes were analyzed by a cold-stage microscope and image analysis at 1, 19.5, and 39 wk of storage. Ice crystal size did not differ among the storage temperatures of light and full-fat ice creams at 19.5 or 39 wk. An increase in ice crystal size was observed between 19.5 and 39 wk for all storage temperatures except -45.6 °C. Coldness intensity, iciness, creaminess, and storage/stale off-flavor of the light and full-fat ice creams were evaluated at 39 wk of storage. Sensory evaluation indicated no difference among the different storage temperatures for light and full-fat ice creams. In a second study, light and full-fat ice creams were heat shocked by storing at -28.9 °C for 35 wk and then alternating between -23.3 and -12.2 °C every 24h for 4 wk. Heat-shocked ice creams were analyzed at 2 and 4 wk of storage for ice crystal size and were evaluated by the sensory panel. A difference in ice crystal size was observed for light and full-fat ice creams during heat-shock storage; however, sensory results indicated no differences. In summary, storage of light or full-fat vanilla-flavored ice creams at the temperatures used within this research did not affect quality of the ice creams. Therefore, ice cream manufacturers could conserve energy by increasing the temperature of freezers from -28.9 to -26.1 °C. Because freezers will typically fluctuate from the set temperature, usage of -26.1 °C allows for a safety factor, even though storage at -23.3 °C did not affect ice cream quality. Copyright © 2011 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

  10. Effects of storage temperature on the physiological characteristics and vegetative propagation of desiccation-tolerant mosses

    NASA Astrophysics Data System (ADS)

    Guo, Yuewei; Zhao, Yunge

    2018-02-01

    Mosses, as major components of later successional biological soil crusts (biocrusts), play many critical roles in arid and semiarid ecosystems. Recently, some species of desiccation-tolerant mosses have been artificially cultured with the aim of accelerating the recovery of biocrusts. Revealing the factors that influence the vegetative propagation of mosses, which is an important reproductive mode of mosses in dry habitats, will benefit the restoration of moss crusts. In this study, three air-dried desiccation-tolerant mosses (Barbula unguiculata, Didymodon vinealis, and Didymodon tectorum) were hermetically sealed and stored at five temperature levels (0, 4, 17, 25, and 30 °C) for 40 days. Then, the vegetative propagation and physiological characteristics of the three mosses were investigated to determine the influence of storage temperature on the vegetative propagation of desiccation-tolerant mosses and the mechanism. The results showed that the vegetative propagation of the three mosses varied with temperature. The most variation in vegetative propagation among storage temperatures was observed in D. tectorum, followed by the variation observed in B. unguiculata. In contrast, no significant difference in propagation among temperatures was found in D. vinealis. The regenerative capacity of the three mosses increased with increasing temperature from 0 to 17 °C, accompanied by a decrease in malondialdehyde (MDA) content, and decreased thereafter. As the temperature increased, the chlorophyll and soluble protein contents increased in B. unguiculata but decreased in D. vinealis and D. tectorum. As to storage, the MDA and soluble sugar contents increased after storage. The MDA content of the three mosses increased at each of the investigated temperatures by more than 50 % from the initial values, and the soluble sugar content became higher than before in the three mosses. The integrity of cells and cell membranes is likely the most important factor influencing the vegetative propagation of desiccation-tolerant mosses. A 40-day storage period caused cell injury. Our results suggest that storage temperature can enhance or suppress such injury and change the regenerative capacity of the three mosses. The data indicate that the suitable storage temperature is 4 °C for B. unguiculata and 17 °C for both D. vinealis and D. tectorum.

  11. Experimental investigation of a molten salt thermocline storage tank

    NASA Astrophysics Data System (ADS)

    Yang, Xiaoping; Yang, Xiaoxi; Qin, Frank G. F.; Jiang, Runhua

    2016-07-01

    Thermal energy storage is considered as an important subsystem for solar thermal power stations. Investigations into thermocline storage tanks have mainly focused on numerical simulations because conducting high-temperature experiments is difficult. In this paper, an experimental study of the heat transfer characteristics of a molten salt thermocline storage tank was conducted by using high-temperature molten salt as the heat transfer fluid and ceramic particle as the filler material. This experimental study can verify the effectiveness of numerical simulation results and provide reference for engineering design. Temperature distribution and thermal storage capacity during the charging process were obtained. A temperature gradient was observed during the charging process. The temperature change tendency showed that thermocline thickness increased continuously with charging time. The slope of the thermal storage capacity decreased gradually with the increase in time. The low-cost filler material can replace the expensive molten salt to achieve thermal storage purposes and help to maintain the ideal gravity flow or piston flow of molten salt fluid.

  12. Storage stability and composition changes of three cold-pressed nut oils under refrigeration and room temperature conditions.

    PubMed

    Rabadán, Adrián; Álvarez-Ortí, Manuel; Pardo, José Emilio; Alvarruiz, Andrés

    2018-09-01

    Chemical composition and stability parameters of three cold-pressed nut oils (almond, walnut and pistachio) were monitored for up to 16 months of storage at 5 °C, 10 °C, 20 °C and room temperature. Freshly pressed pistachio oil had lower peroxide value than almond oil and higher induction period than almond and walnut oils, indicating a higher stability. The peroxide values increased faster at room temperature than at lower temperatures during the storage time, and the highest increase was for pistachio oil stored at room temperature exposed to daylight. The induction period decreased for all three nut oils during the storage time, regardless of the storage conditions. Pistachio oil remained the most stable oil at the end of the storage time, followed by almond oil. The percentage of polyunsaturated fatty acids decreased slightly throughout the storage. Copyright © 2018 Elsevier Ltd. All rights reserved.

  13. Carotenoid accumulation in postharvest "Cara Cara" navel orange (Citrus sinensis Osbeck) fruits stored at different temperatures was transcriptionally regulated in a tissue-dependent manner.

    PubMed

    Tao, Nengguo; Wang, Changfeng; Xu, Juan; Cheng, Yunjiang

    2012-09-01

    The main objective of this work was to investigate the effect of storage temperature (4 and 20 °C) on carotenoid accumulation and on the expression levels of seven carotenoid biosynthetic genes (Psy, Pds, Zds, Lcyb, Lcye, Hyb and Zep) in postharvest 'Cara Cara' navel orange (C. sinensis Osbeck) fruits. Storage at 20 °C rapidly increased the carotenoid content in the peel, whereas the content remained unchanged in the pulp before 35 days of storage. By contrast, storage at 4 °C maintained the carotenoid content in the peel before 35 days of storage, after which it slightly increased as time progressed. However, the content in the pulp gradually increased over the entire storage period. In the peel, the gene expressions of Psy and Lcyb were up-regulated at 20 °C but remained unchanged at 4 °C. In addition, the gene expressions of Zds, Hyb, and Zep were repressed at both temperatures before the early storage, followed by a rapid increase only at 20 °C. Then the expressions remained constant level at both temperatures, with the expression level at 20 °C higher than that at 4 °C. Low temperature (4 °C) apparently induced the expression of all the test carotenoid biosynthetic genes in the pulp, in contrast to the nearly stable level at 20 °C. Our present study suggests that the carotenoid biosynthesis in postharvest 'Cara Cara' fruits is transcriptionally regulated, and storage temperature affects the carotenoid accumulation and gene expression in a tissue-dependent manner. Temperature could affect the carotenoid biosynthesis in postharvest 'Cara Cara' fruits in a tissue-dependent manner. The carotenoid biosynthesis in postharvest 'Cara Cara' fruits was transcriptionally regulated by correlated genes.

  14. Unraveling protein stabilization mechanisms: vitrification and water replacement in a glass transition temperature controlled system.

    PubMed

    Grasmeijer, N; Stankovic, M; de Waard, H; Frijlink, H W; Hinrichs, W L J

    2013-04-01

    The aim of this study was to elucidate the role of the two main mechanisms used to explain the stabilization of proteins by sugar glasses during drying and subsequent storage: the vitrification and the water replacement theory. Although in literature protein stability is often attributed to either vitrification or water replacement, both mechanisms could play a role and they should be considered simultaneously. A model protein, alkaline phosphatase, was incorporated in either inulin or trehalose by spray drying. To study the storage stability at different glass transition temperatures, a buffer which acts as a plasticizer, ammediol, was incorporated in the sugar glasses. At low glass transition temperatures (<50°C), the enzymatic activity of the protein strongly decreased during storage at 60°C. Protein stability increased when the glass transition temperature was raised considerably above the storage temperature. This increased stability could be attributed to vitrification. A further increase of the glass transition temperature did not further improve stability. In conclusion, vitrification plays a dominant role in stabilization at glass transition temperatures up to 10 to 20°C above storage temperature, depending on whether trehalose or inulin is used. On the other hand, the water replacement mechanism predominantly determines stability at higher glass transition temperatures. Copyright © 2013 Elsevier B.V. All rights reserved.

  15. The effect of storage temperature on blue cheese mechanical properties.

    PubMed

    Joyner Melito, Helen S; Francis, Dorothy; Luzzi, Brooke; Johnson, John R

    2018-06-01

    Blue cheese is commonly aged for 60 days at 10°C after curing. However, some manufacturers store blue cheese at 4°C and the effect of lower storage temperature on blue cheese final properties is unknown. Thus, the objective of this study was to determine the effect of storage temperature and time on blue cheese mechanical behaviors. Blue cheeses were stored at 4 or 10°C for 77 days after production. Composition and small- and large-strain rheological behaviors were evaluated every 2 weeks of storage. Storage time had significant impact on blue cheese rheological behaviors; storage temperature did not. Large-strain compressive force and viscoelastic moduli decreased with storage time, and the extent of nonlinear viscoelastic behavior increased. These results indicated that sample microstructure likely weakened and was more easily deformed as storage time increased. Overall, blue cheese can be stored at 4-10°C without significant changes to its composition or mechanical behavior. The results of this work can be used by blue cheese manufacturers to better understand the impact of storage time and temperature on blue cheese end quality. Manufacturers can take advantage of the effects of storage time on blue cheese mechanical behaviors to determine how long to age blue cheese to achieve the desired texture. © 2017 Wiley Periodicals, Inc.

  16. Effects of storage time and temperature on pH, specific gravity, and crystal formation in urine samples from dogs and cats.

    PubMed

    Albasan, Hasan; Lulich, Jody P; Osborne, Carl A; Lekcharoensuk, Chalermpol; Ulrich, Lisa K; Carpenter, Kathleen A

    2003-01-15

    To determine effects of storage temperature and time on pH and specific gravity of and number and size of crystals in urine samples from dogs and cats. Randomized complete block design. 31 dogs and 8 cats. Aliquots of each urine sample were analyzed within 60 minutes of collection or after storage at room or refrigeration temperatures (20 vs 6 degrees C [68 vs 43 degrees F]) for 6 or 24 hours. Crystals formed in samples from 11 of 39 (28%) animals. Calcium oxalate (CaOx) crystals formed in vitro in samples from 1 cat and 8 dogs. Magnesium ammonium phosphate (MAP) crystals formed in vitro in samples from 2 dogs. Compared with aliquots stored at room temperature, refrigeration increased the number and size of crystals that formed in vitro; however, the increase in number and size of MAP crystals in stored urine samples was not significant. Increased storage time and decreased storage temperature were associated with a significant increase in number of CaOx crystals formed. Greater numbers of crystals formed in urine aliquots stored for 24 hours than in aliquots stored for 6 hours. Storage time and temperature did not have a significant effect on pH or specific gravity. Urine samples should be analyzed within 60 minutes of collection to minimize temperature- and time-dependent effects on in vitro crystal formation. Presence of crystals observed in stored samples should be validated by reevaluation of fresh urine.

  17. Yield and cold storage of Trichoderma conidia is influenced by substrate pH and storage temperature.

    PubMed

    Steyaert, Johanna M; Chomic, Anastasia; Nieto-Jacobo, Maria; Mendoza-Mendoza, Artemio; Hay, Amanda J; Braithwaite, Mark; Stewart, Alison

    2017-05-01

    In this study we examined the influence of the ambient pH during morphogenesis on conidial yield of Trichoderma sp. "atroviride B" LU132 and T. hamatum LU593 and storage at low temperatures. The ambient pH of the growth media had a dramatic influence on the level of Trichoderma conidiation and this was dependent on the strain and growth media. On malt-extract agar, LU593 yield decreased with increasing pH (3-6), whereas yield increased with increasing pH for LU132. During solid substrate production the reverse was true for LU132 whereby yield decreased with increasing pH. The germination potential of the conidia decreased significantly over time in cold storage and the rate of decline was a factor of the strain, pH during morphogenesis, growth media, and storage temperature. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  18. Recovery of low-temperature stressed E. coli O157:H7 and its susceptibility to crystal violet, bile salt, sodium chloride and ethanol.

    PubMed

    Chou, C C; Cheng, S J

    2000-11-01

    This study was conducted to investigate the alteration of some characteristics of E. coli O157:H7 subjected to various periods of storage at -5, -18 and -28 degrees C. Results revealed that the low-temperature treatments increased the susceptibility of E. coli O157:H7 to crystal violet, bile salt, sodium chloride and ethanol. In general, the susceptibility of E. coli O157:H7 subjected to storage at -18 degrees C increased most significantly. The susceptibility of E. coli O157:H7 to the tested agents increased as the period of low-temperature storage extended, regardless of storage temperature. Among the various nitrogen and carbon sources tested, tryptone and soytone were the most effective nitrogen sources, while glucose and maltose were the most effective carbon sources for the growth of the low-temperature stressed cells. When growing the stressed E. coli O157:H7 in media containing the same nitrogen source or carbon source, their lag period increased as the time of frozen storage increased. It was also noted that in general, the recovery of the low-temperature stressed E. coli O157:H7 was highest on tryptic soy agar followed by Modified eosin methylene blue agar, while recovery on MaConkey sorbitol agar and Modified MaConkey sorbitol agar was lowest.

  19. Ecosystem carbon storage does not vary with increasing mean annual temperature in Hawaiian tropical montane wet forests

    Treesearch

    Paul Selmants; Creighton Litton; Christian P. Giardina; Greg P. Asner

    2014-01-01

    Theory and experiment agree that climate warming will increase carbon fluxes between terrestrial ecosystems and the atmosphere. The effect of this increased exchange on terrestrial carbon storage is less predictable, with important implications for potential feedbacks to the climate system. We quantified how increased mean annual temperature (MAT) affects ecosystem...

  20. Temperature-Dependent Lipid Storage of Juvenile Arctic cod (Boreogadus saida) and Co-Occurring North Pacific Gadids

    NASA Astrophysics Data System (ADS)

    Copeman, L.; Laurel, B.; Spencer, M. L.; Iseri, P.; Sremba, A. L.

    2016-02-01

    Climate change impacts on Arctic ecosystems will largely be determined by temperature-dependent bioenergetics of resident and invading forage fish species. In this study, we experimentally measured total lipids and lipid class storage in the liver and muscle of juvenile Arctic gadids (Arctic cod, Boreogadus saida and saffron cod, Eleginus gracilis) and two North Pacific gadids (walleye pollock, Gadus chalcogrammus and Pacific cod, Gadus macrocephalus). Experiments were conducted over a 6-wk period across five temperatures (0, 5, 9, 16 and 20 °C) at the Hatfield Marine Science Center in Newport, OR, USA. Results indicated clear physiological differences among species in terms of temperature-dependent growth and lipid storage. Arctic cod exhibited highest growth and lipid storage (27 mg/g WW) at the coldest temperature (0 °C) compared to the other gadids, with near maximum growth at 5 °C and onset of mortality above 9 °C. In contrast, saffron cod growth rates steadily increased at temperatures beyond 16 °C, but lipid storage was low overall with only slightly higher lipid storage at warm temperatures (10 to 17 mg/g WW). Both walleye pollock and Pacific cod showed a domed response with increased lipid storage and growth at intermediate temperatures (9 - 12°C) and reduced growth and lipid storage at cold and warm maxima. We did not observe a trade-off between growth rate and lipid accumulation in any species. These results suggest that saffron cod can thrive in a warming Arctic but will be energetically inferior as a prey item to the more temperature-sensitive Arctic cod. Alternatively, North Pacific gadids can energetically resemble Arctic cod at warmer temperatures and could theoretically be an important prey item if their range extends northward with continued climate change.

  1. The combined effects of storage temperature and packaging on the sensory, chemical, and physical properties of a Cabernet Sauvignon wine.

    PubMed

    Hopfer, Helene; Buffon, Peter A; Ebeler, Susan E; Heymann, Hildegarde

    2013-04-03

    A Californian Cabernet Sauvignon was stored for 6 months at three different constant temperatures to study the combined effects of storage temperature and packaging configuration. Glass bottles with natural cork, synthetic cork, and screw cap closure, as well as two Bag-in-Box treatments, were used in the experiment. A trained sensory panel was able to detect significant changes in aroma, flavor, taste, mouthfeel, and color attributes among the samples, differences that were found also with various chemical and physical measurements (volatile profile, polyphenol pattern, enological parameters, color space). Additionally, two commonly used polyphenol assays were compared to each other in terms of their ability to detect the changes in the polyphenol profile. Generally, sample changes were more pronounced due to the different storage temperatures, with 30 sensory attributes differing significantly among the three different storage temperatures, while only 17 sensory attributes showed a significant packaging effect. With increasing storage temperature the packaging effect became more pronounced, resulting in the largest changes in the Bag-in-Box samples stored at the highest temperature of 40 °C. At the highest storage temperature, all wines showed oxidized characters, independent of the wine packaging configurations, but to a varying degree. Generally, wines that received highest oxygen amounts and storage temperatures were much lighter, less red, and more brown-yellow at the end of the 6-month storage period, compared to their counterparts stored at 10 °C. These changes in color and polyphenols, respectively, were also detected with the two spectrophotometric assays. With increasing storage temperature both assays measured reduced concentrations in total phenols and total anthocyanins, while total tannins, degree of ionized anthocyanins, and color density increased. Various volatile compounds differed significantly among the samples, with largest relative concentration changes in acetates, organic acids, and alcohols, in good agreement with previous literature reports, with some being well correlated to specific sensory attributes too; for example, various acetates correlated to cherry and fruit aromas and flavors. The study shows that storage at elevated temperatures could be a valuable tool for wine packaging screening and testing new and improved wine packaging types under the worst conditions, which are unfortunately not unrealistic.

  2. Pressure and temperature effects on degradation kinetics and storage stability of total anthocyanins in blueberry juice.

    PubMed

    Buckow, Roman; Kastell, Anja; Terefe, Netsanet Shiferaw; Versteeg, Cornelis

    2010-09-22

    The degradation kinetics of total anthocyanins in blueberry (Vaccinium myrtillus) juice were studied during thermal processing by treatment at selected temperatures (60-121 °C) and combined high pressure-temperature processing (100-700 MPa, 40-121 °C). Anthocyanin stability was also studied for several of these treatments during storage at 4, 25, and 40 °C. Both pressure and temperature increased d, the degradation rate of total anthocyanins in blueberry juice, meaning that at constant temperature, anthocyanins were more rapidly degraded with increasing pressure. For example, 32% degradation of anthocyanins was observed after 20 min heating at 100 °C and atmospheric pressure, whereas at 100 °C and 600 MPa, approximately 50% of total anthocyanins were lost. Degradation of anthocyanins was significantly accelerated with increasing storage temperatures. Combined pressure-temperature treatment of pasteurized juice led to a slightly faster degradation of total anthocyanins during storage compared to heat treatments at ambient pressure. Degradation of anthocyanins was best described by a 1.4th-order reaction at all conditions investigated. A mathematical model describing the degradation of blueberry anthocyanins in juice as a function of pressure, temperature, and treatment time is presented.

  3. Cloning and expression of γ-glutamyl transpeptidase and its relationship to greening in crushed garlic (Allium sativum) cloves.

    PubMed

    Cho, Jungeun; Park, Minkyu; Choi, Doil; Lee, Seung Koo

    2012-01-30

    Garlic greening occurs when garlic cloves are stored at low temperature, increasing 1-propenyl cysteine sulfoxide, which is induced by γ-glutamyl transpeptidase (GGT) activity. Although the metabolism of the γ-glutamyl peptide is important for the biosynthesis of green pigments in crushed garlic cloves, garlic GGT is poorly characterised. For the analysis of GGT at the gene level, the garlic GGT sequence was partially cloned using an onion GGT sequence. The relationship between garlic greening and related gene expressions, depending on storage condition, was investigated using reverse transcription polymerase chain reaction for garlic GGT and alliinase. Three storage conditions were set: A, storage at a constant temperature of 20 °C; B, storage at 20 °C for 3 months and then transfer to 0 °C for an additional 3 months; C, storage at 0 °C for 3 months and then transfer to 20 °C for an additional 3 months. GGT expression increased under storage condition B and decreased under storage condition C. However, alliinase expression was not affected by storage condition. Greening in crushed garlic cloves increases with increasing GGT expression at low temperature, while alliinase expression is not affected. Copyright © 2011 Society of Chemical Industry.

  4. Effect of long term chilled (up to 5weeks) then frozen (up to 12months) storage at two different sub-zero holding temperatures on beef: 2. Lipid oxidation and fatty acid profiles.

    PubMed

    Holman, Benjamin W B; Coombs, Cassius E O; Morris, Stephen; Bailes, Kristy; Hopkins, David L

    2018-02-01

    Lipid oxidation and fatty acid (FA) profiles were evaluated for beef M. longissimus lumborum (LL) stored under different chilled-then-frozen storage combinations (up to 5 and 52weeks, respectively) and two frozen holding temperatures (-12°C and -18°C). FA profile variation was observed, with increasing frozen storage periods resulting in unsaturated FA levels declining as saturated FA levels increased. Polyunsaturated and health claimable FA levels also tended to decline with increasing chilled storage period, albeit insignificant within the constraints of the experimental design. Peroxidase activity, TBARS and oxidation-reduction potential analyses reflected these FA changes. These, when compared against existing consumer thresholds, suggest a perceptible detraction from LL held under long-term frozen storage durations that are less evident earlier as dependent on the preceding chilled storage period. Negligible impact of frozen storage holding temperatures was observed on measured traits. These results suggest long-term chilled-then-frozen storage can influence beef lipid stability, healthy FA profile and therefore the healthiness of beef. Crown Copyright © 2017. Published by Elsevier Ltd. All rights reserved.

  5. The storage capacity of fluorine in olivine and pyroxene under upper mantle conditions

    NASA Astrophysics Data System (ADS)

    Grützner, Tobias; Kohn, Simon C.; Bromiley, David W.; Rohrbach, Arno; Berndt, Jasper; Klemme, Stephan

    2017-07-01

    We present new experimental results on the fluorine storage capacity of olivine and orthopyroxene in the Earth's mantle. Experiments were performed in the system MgO-SiO2 + MgF2 at temperatures between 1350 °C and 1700 °C and pressures up to 17 GPa. Electron microprobe measurements show that fluorine concentrations in olivine reach up to 5100 μg/g. The storage capacity of fluorine in olivine shows only a small pressure dependence but a strong temperature dependence with a positive correlation between increasing temperature and fluorine storage capacity. Fluorine concentrations found in enstatite are one order of magnitude smaller and reach up to 670 μg/g. Our data show that concentrations of fluorine in fluorine-saturated olivine are in the same range as water concentrations in olivine. Nevertheless, fluorine and water solubility in olivine show opposing behavior with increasing pressure and temperature. The fluorine solubility in olivine increases with increasing temperature but is not much affected by pressure. In contrast, water solubility in olivine has previously been shown to decrease with increasing temperature and increase with increasing pressure. Our experiments show that nominally fluorine-free minerals like forsterite and enstatite are capable of storing the entire fluorine budget of the upper mantle, without the need to invoke accessory phases such as apatite or amphibole.

  6. Influence of preservation methods on the quality of colostrum sourced from New Zealand dairy farms.

    PubMed

    Denholm, K S; Hunnam, J C; Cuttance, E L; McDougall, S

    2017-09-01

    To assess the effect of two temperatures (ambient temperature and 4°C), three preservation methods (no preservative, yoghurt and potassium sorbate), and two periods of storage (3 and 7 days) on Brix and total bacterial and coliform counts of colostrum collected from New Zealand dairy farms. One litre of colostrum destined to be fed to newborn calves was collected from 55 New Zealand dairy farms in the spring of 2015. Six aliquots of 150 mL were obtained from each colostrum sample, with two aliquots left untreated, two treated with potassium sorbate and two with yoghurt, and one of each pair of aliquots stored at ambient temperature and the other at 4°C. All samples were tested for Brix, total bacterial counts and coliform counts before treatment (Day 0), and after 3 and 7 days of storage. The effect of preservation method and storage temperature on the change in Brix, bacterial and coliform counts after 3 or 7 days of storage was analysed using multivariable random effects models. For all outcome variables there was a temperature by preservation interaction. For aliquots preserved with potassium sorbate, changes in Brix and bacterial counts did not differ between aliquots stored at ambient temperature or 4°C, but for aliquots preserved with yoghurt or no preservative the decrease in Brix and increase in bacterial counts was greater for aliquots stored at ambient temperature than 4°C (p<0.001). For aliquots preserved with potassium sorbate, coliform counts decreased at both temperatures, but for aliquots preserved with yoghurt or no preservative coliform counts increased for aliquots stored at 4°C, but generally decreased at ambient temperatures (p<0.001). There was also an interaction between duration of storage and temperature for bacterial counts (p<0.001). The difference in the increase in bacterial counts between aliquots stored at 4°C and ambient temperature after 3 days was greater than between aliquots stored at 4°C and ambient temperature after 7 days. Use of potassium sorbate to preserve colostrum for 3 or 7 days resulted in little or no reduction in Brix and a lower increase in total bacterial counts than colostrum stored without preservative or with yoghurt added. Colostrum quality was not affected by storage temperature for samples preserved with potassium sorbate, but storage at 4°C resulted in better quality colostrum than storage at ambient temperatures for colostrum with no preservative or yoghurt added.

  7. Changes in nitrate and nitrite content of four vegetables during storage at refrigerated and ambient temperatures.

    PubMed

    Chung, J-C; Chou, S-S; Hwang, D-F

    2004-04-01

    The nitrate and nitrite contents of four kinds of vegetables (spinach, crown daisy, organic Chinese spinach and organic non-heading Chinese cabbage) in Taiwan were determined during storage at both refrigerated (5 +/- 1 degrees C) and ambient temperatures (22 +/- 1 degrees C) for 7 days. During storage at ambient temperature, nitrate levels in the vegetables dropped significantly from the third day while nitrite levels increased dramatically from the fourth day of storage. However, refrigerated storage did not lead to changes in nitrate and nitrite levels in the vegetables over 7 days.

  8. Changes in Biochemical Characteristics and Activities of Ripening Associated Enzymes in Mango Fruit during the Storage at Different Temperatures

    PubMed Central

    Kimura, Yoshinobu

    2014-01-01

    As a part of the study to explore the possible strategy for enhancing the shelf life of mango fruits, we investigated the changes in biochemical parameters and activities of ripening associated enzymes of Ashwina hybrid mangoes at 4-day regular intervals during storage at −10°C, 4°C, and 30 ± 1°C. Titratable acidity, vitamin C, starch content, and reducing sugar were higher at unripe state and gradually decreased with the increasing of storage time at all storage temperatures while phenol content, total soluble solid, total sugar, and nonreducing sugar contents gradually increased. The activities of amylase, α-mannosidase, α-glucosidase, and invertase increased sharply within first few days and decreased significantly in the later stage of ripening at 30 ± 1°C. Meanwhile polyphenol oxidase, β-galactosidase, and β-hexosaminidase predominantly increased significantly with the increasing days of storage till later stage of ripening. At −10°C and 4°C, the enzymes as well as carbohydrate contents of storage mango changed slightly up to 4 days and thereafter the enzyme became fully dormant. The results indicated that increase in storage temperature and time correlated with changes in biochemical parameters and activities of glycosidases suggested the suppression of β-galactosidase and β-hexosaminidase might enhance the shelf life of mango fruits. PMID:25136564

  9. Changes in dark chocolate volatiles during storage.

    PubMed

    Nightingale, Lia M; Cadwallader, Keith R; Engeseth, Nicki J

    2012-05-09

    Chocolate storage is critical to the quality of the final product. Inadequate storage, especially with temperature fluctuations, may lead to a change in crystal structure, which may eventually cause fat bloom. Bloom is the main cause of quality loss in the chocolate industry. The impact of various storage conditions on the flavor quality of dark chocolate was determined. Dark chocolate was stored in different conditions leading to either fat or sugar bloom and analyzed at 0, 4, and 8 weeks of storage. Changes in chocolate flavor were determined by volatile analysis and descriptive sensory evaluation. Results were analyzed by analysis of variance (ANOVA), cluster analysis, principal component analysis (PCA), and linear partial least-squares regression analysis (PLS). Volatile concentration and loss were significantly affected by storage conditions. Chocolates stored at high temperature were the most visually and texturally compromised, but volatile concentrations were affected the least, whereas samples stored at ambient, frozen, and high relative humidity conditions had significant volatile loss during storage. It was determined that high-temperature storage caused a change in crystal state due to the polymorphic shift to form VI, leading to an increase in sample hardness. Decreased solid fat content (SFC) during high-temperature storage increased instrumentally determined volatile retention, although no difference was detected in chocolate flavor during sensory analysis, possibly due to instrumental and sensory sampling techniques. When all instrumental and sensory data had been taken into account, the storage condition that had the least impact on texture, surface roughness, grain size, lipid polymorphism, fat bloom formation, volatile concentrations, and sensory attributes was storage at constant temperature and 75% relative humidity.

  10. Temperature Control of Hypertensive Rats during Moderate Exercise in Warm Environment.

    PubMed

    Campos, Helton O; Leite, Laura H R; Drummond, Lucas R; Cunha, Daise N Q; Coimbra, Cândido C; Natali, Antônio J; Prímola-Gomes, Thales N

    2014-09-01

    The control of body temperature in Spontaneously Hypertensive Rat (SHR) subjected to exercise in warm environment was investigated. Male SHR and Wistar rats were submitted to moderate exercise in temperate (25°C) and warm (32°C) environments while body and tail skin temperatures, as well as oxygen consumption, were registered. Total time of exercise, workload performed, mechanical efficiency and heat storage were determined. SHR had increased heat production and body temperature at the end of exercise, reduced mechanical efficiency and increased heat storage (p < 0.05). Furthermore, these rats also showed a more intense and faster increase in body temperature during moderate exercise in the warm environment (p < 0.05). The lower mechanical efficiency seen in SHR was closely correlated with their higher body temperature at the point of fatigue in warm environment (p < 0.05). Our results indicate that SHR exhibit significant differences in body temperature control during moderate exercise in warm environment characterized by increased heat production and heat storage during moderate exercise in warm environment. The combination of these responses result in aggravated hyperthermia linked with lower mechanical efficiency. Key PointsThe practice of physical exercise in warm environment has gained importance in recent decades mainly because of the progressive increases in environmental temperature;To the best of our knowledge, these is the first study to analyze body temperature control of SHR during moderate exercise in warm environment;SHR showed increased heat production and heat storage that resulted in higher body temperature at the end of exercise;SHR showed reduced mechanical efficiency;These results demonstrate that when exercising in a warm environment the hypertensive rat exhibit differences in temperature control.

  11. Guidelines for maintaining and managing the vaccine cold chain.

    PubMed

    2003-10-24

    In February 2002, the Advisory Committee on Immunization Practices (ACIP) and American Academy of Family Physicians (AAFP) released their revised General Recommendations on Immunization, which included recommendations on the storage and handling of immunobiologics. Because of increased concern over the potential for errors with the vaccine cold chain (i.e., maintaining proper vaccine temperatures during storage and handling to preserve potency), this notice advises vaccine providers of the importance of proper cold chain management practices. This report describes proper storage units and storage temperatures, outlines appropriate temperature-monitoring practices, and recommends steps for evaluating a temperature-monitoring program. The success of efforts against vaccine-preventable diseases is attributable in part to proper storage and handling of vaccines. Exposure of vaccines to temperatures outside the recommended ranges can affect potency adversely, thereby reducing protection from vaccine-preventable diseases. Good practices to maintain proper vaccine storage and handling can ensure that the full benefit of immunization is realized.

  12. Effect of storage conditions on the calorific value of municipal solid waste.

    PubMed

    Nzioka, Antony Mutua; Hwang, Hyeon-Uk; Kim, Myung-Gyun; Yan, Cao Zheng; Lee, Chang-Soo; Kim, Young-Ju

    2017-08-01

    Storage conditions are considered to be an important factor as far as waste material characteristics are concerned. This experimental investigation was conducted using municipal solid waste (MSW) with a high moisture content and varying composition of organic waste. The objective of this study was to understand the effect of storage conditions and temperature on the moisture content and calorific value of the waste. Samples were subjected to two different storage conditions and investigated at specified temperatures. The composition of sample materials investigated was varied for each storage condition and temperature respectively. Gross calorific value was determined experimentally while net calorific value was calculated using empirical formulas proposed by other researchers. Results showed minimal changes in moisture content as well as in gross and net calorific values when the samples were subjected to sealed storage conditions. Moisture content reduced due to the ventilation process and the rate of moisture removal increased with a rise in storage temperature. As expected, rate of moisture removal had a positive effect on gross and net calorific values. Net calorific values also increased at varying rates with a simultaneous decrease in moisture content. Experimental investigation showed the effectiveness of ventilation in improving the combustion characteristics of the waste.

  13. The Effects of Storage Temperature on the Growth of Vibrio parahaemolyticus and Organoleptic Properties in Oysters.

    PubMed

    Mudoh, Meshack Fon; Parveen, Salina; Schwarz, Jurgen; Rippen, Tom; Chaudhuri, Anish

    2014-01-01

    During harvesting and storage, microbial pathogens and natural spoilage flora may grow, negatively affecting the composition and texture of oysters and posing a potential health threat to susceptible consumers. A solution to these problems would mitigate associated damaging effects on the seafood industry. The purpose of this study was to investigate the effects of storage temperature on growth of vibrios as well as other microbial, sensory, and textural characteristics of post-harvest shellstock Eastern oysters (Crassostrea virginica). Oysters harvested from the Chesapeake Bay, Maryland, during summer months (June, July, and August, 2010) were subjected to three storage temperatures (5, 10, and 20°C) over a 10-day period. At selected time intervals (0, 1, 3, 7, and 10 days), two separate samples of six oysters each were homogenated and analyzed for pH, halophilic plate counts (HPC), total vibrios, and Vibrio parahaemolyticus (Vp). Oyster meats shucked after storage were also organoleptically evaluated (acceptability, appearance, and odor). Texture analysis was performed using a texture analyzer on meats shucked from oysters held under the same conditions. The pH of the oyster homogenates showed no consistent pattern with storage time and temperature. The HPC (4.5-9.4 log CFU/g) were highest on day 7 at 20°C while olfactory acceptance reduced with time and increasing storage temperatures. The Vp counts increased over time from 3.5 to 7.5 log MPN/g by day 10. Loss of freshness as judged by appearance and odor was significant over time (p < 0.05). Toughness of oysters increased with storage time at 5 and 10°C from days 1 to 3 but was inconsistent after day 7. The results indicate that the length of storage and temperature had a significant effect on bacterial counts and olfactory acceptance of oysters but had an inconsistent effect on texture.

  14. Temperature dependency of the thermal conductivity of porous heat storage media

    NASA Astrophysics Data System (ADS)

    Hailemariam, Henok; Wuttke, Frank

    2018-04-01

    Analyzing the variation of thermal conductivity with temperature is vital in the design and assessment of the efficiency of sensible heat storage systems. In this study, the temperature variation of the thermal conductivity of a commercial cement-based porous heat storage material named - Füllbinder L is analyzed in saturated condition in the temperature range between 20 to 70°C (water based storage) with a steady state thermal conductivity and diffusivity meter. A considerable decrease in the thermal conductivity of the saturated sensible heat storage material upon increase in temperature is obtained, resulting in a significant loss of system efficiency and slower loading/un-loading rates, which when unaccounted for can lead to the under-designing of such systems. Furthermore, a new empirical prediction model for the estimation of thermal conductivity of cement-based porous sensible heat storage materials and naturally occurring crystalline rock formations as a function of temperature is proposed. The results of the model prediction are compared with the experimental results with satisfactory results.

  15. Evaporative cooling system for storage of fruits and vegetables - a review.

    PubMed

    Lal Basediya, Amrat; Samuel, D V K; Beera, Vimala

    2013-06-01

    Horticultural produce are stored at lower temperature because of their highly perishable nature. There are many methods to cool the environment. Hence, preserving these types of foods in their fresh form demands that the chemical, bio-chemical and physiological changes are restricted to a minimum by close control of space temperature and humidity. The high cost involved in developing cold storage or controlled atmosphere storage is a pressing problem in several developing countries. Evaporative cooling is a well-known system to be an efficient and economical means for reducing the temperature and increasing the relative humidity in an enclosure and this effect has been extensively tried for increasing the shelf life of horticultural produce in some tropical and subtropical countries. In this review paper, basic concept and principle, methods of evaporative cooling and their application for the preservation of fruits and vegetables and economy are also reported. Thus, the evaporative cooler has prospect for use for short term preservation of vegetables and fruits soon after harvest. Zero energy cooling system could be used effectively for short-duration storage of fruits and vegetables even in hilly region. It not only reduces the storage temperature but also increases the relative humidity of the storage which is essential for maintaining the freshness of the commodities.

  16. Effect of processing conditions on the quality characteristics of barley chips.

    PubMed

    Prakash, Jyoti; Naik, H R; Hussain, Syed Zameer; Singh, Baljit

    2015-01-01

    The aim of the present study was to study the effect of lime concentration, frying temperature and frying time on quality characteristics of barley chips. Effect of salt concentration and packaging material on the quality and stability of the product was also studied during 180 days of storage under ambient conditions. An increase in fat content of chips was observed with the increase in lime concentration, frying temperature and time, whereas a decreasing trend was observed in moisture content of chips. An increase in amylose content of chips was observed during frying. However, it was found that the amylopectin in chips decreased during frying as frying temperature and time was increased. An increase in colour difference (ΔE) and crispness was noted in chips during frying as frying temperature and time increased. With the increase in lime concentration (0.5 and 1.0 %) both ΔE and break force of chips was found decreased. The results further revealed that there was gradual decrease in fat (%) and amylopectin (%) during storage, whereas moisture (%) and amylose (%) increased during storage period. Organoleptic evaluation of the product revealed that scores of colour, texture, flavour and over all acceptability decrease during storage. However the treatment (salt 2 % and aluminium based laminate) recorded better score with respect to colour, flavour, texture and overall acceptability.

  17. [Effects of low temperature on dormancy breaking and growth after planting in bulbs of Tulipa edulis].

    PubMed

    Yang, Ying; Zhu, Zai-Biao; Guo, Qiao-Sheng; Miao, Yuan-Yuan; Ma, Hong-Liang; Yang, Xiao-Hua

    2015-01-01

    The effect of low temperature storage on dormancy breaking, sprouting and growth after planting of Tulipa edulis was studied. The results showed that starch content and activity of amylases significantly decreased during 10 weeks of cold storage, soluble protein content raised at first then decreased, and the peak appeared at the 6th week. However, total soluble sugar content which in- creased slowly at first than rose sharply and reducing sugar content increased during the storage duration. The bulbs with cold storage treatment rooted in the 6th week, which was about 2 weeks earlier than room temperature storage, but there were less new roots in the late period of storage. After stored at a low temperature, bud lengths were longer than that with room temperature treatment. Cold storage treatment could promote earlier emergence, shorten germination time, prolong growth period and improve the yield of bulb, but rarely affect the emergence rate. It was not beneficial to flowering and fruiting. The results indicated that 6-8 weeks of cold storage was deemed to be the key period of dormancy breaking preliminary.

  18. Cooking quality properties and free and bound phenolics content of brown, black, and red rice grains stored at different temperatures for six months.

    PubMed

    Ziegler, Valmor; Ferreira, Cristiano Dietrich; Hoffmann, Jessica Fernanda; Chaves, Fábio Clasen; Vanier, Nathan Levien; de Oliveira, Maurício; Elias, Moacir Cardoso

    2018-03-01

    The changes in cooking quality and phenolic composition of whole black and red rice grains stored during six months at different temperatures were evaluated. Brown rice with known cooking quality properties and low phenolic levels was used for purposes comparison. All rice genotypes were stored at 13% moisture content at temperatures of 16, 24, 32, and 40°C. Cooking time, hardness, free and bound phenolics, anthocyanins, proanthocyanidins, and free radical scavenging capacity were analysed. The traditional rice with brown pericarp exhibited an increase in cooking time and free phenolics content, while rice with black pericarp exhibited a reduction in cooking time after six months of storage at the highest studied temperature of 40°C. There as increases in ferulic acid levels occurred as a function of storage temperature. Red pericarp rice grains showed decreased antioxidant capacity against ABTS radical for the soluble phenolic fraction with increased time and storage temperature. Copyright © 2017 Elsevier Ltd. All rights reserved.

  19. Control of rotting and browning of Longan fruit cv. Biew Kiew after harvested by sulphur dioxide treatment under various storage temperatures.

    PubMed

    Chitbanchong, W; Sardsud, V; Whangchai, K; Koslanund, R; Thobunluepop, P

    2009-11-15

    The experiment of Longan fruit cv. Biew Kiew, untreated (control) and treated with SO2 treatment were stored under 2 +/- 2 and 7 +/- 2 degrees C for 0, 2, 4, 6 and 8 weeks were studied. The treatment of fresh longan fruit with SO2 fumigation combined with the suitable storage condition improved the overall longan fruit quality, especially on inner and outer peel tissue and aril color than no SO2 treatment. Treatment stabilizes peel color with no subsequent loss of color during storage (fruit color were bright-yellowish color). When the fruit showed during SO2 treatment, increasing of storage duration and temperatures, the dark color of inner and outer peel of longan fruit was appeared, this was correlated with the increasing of PPO activity. The activity of PPO enzyme in control fruit (no SO2 treatment) gradually lower than SO2 treatments. Fruit exposed to cool storage temperature (2 degrees C) exhibited a lower PPO enzymatic activity compared to those kept in high storage temperature (7 degrees C). Moreover, PPO enzymatic activity significantly increased over the storage durations The additional SO2 treatment no subsequent loss of weight of longan fruit during storage. However, the sulphite residues could detect immediately after SO2 treatment in all part of longan fruit, especially on aril tissue. The SEM evaluation found that the surface cracking was also impair the physiological function of the cuticle and increasing water permeability, which may cause water soaking at the inner side of the peel. The injured cell would accelerate the oxidation of phenolic substances and the oxidative products resulted in dark color of inner and outer peel.

  20. Low-technology cooling box for storage of malaria RDTs and other medical supplies in remote areas.

    PubMed

    Chanthap, Lon; Ariey, Frédéric; Socheat, Duong; Tsuyuoka, Reiko; Bell, David

    2010-01-23

    With the increase in use of point-of-care diagnostic tests for malaria and other diseases comes the necessity of storing the diagnostic kits and the drugs required for subsequent management, in remote areas, where temperatures are high and electricity supply is unreliable or unavailable. To address the lack of temperature-controlled storage during the introduction of community-based malaria management in Cambodia, the Cambodian National Centre for Parasitology, Entomology and Malaria Control (CNM) developed prototype evaporative cooling boxes (Cambodian Cooler Boxes - CCBs) for storage of perishable medical commodities in remote clinics. The performance of these CCBs for maintaining suitable storage temperatures was evaluated over two phases in 2005 and 2006-7, comparing conditions in CCBs using water as designed, CCBs with no water for evaporation, and ambient storage room temperatures. Temperature and humidity was monitored, together with the capacity of the RDTs recommended for storage between 2 to 30 degree Celsius to detect low-density malaria parasite samples after storage under these conditions. Significant differences were recorded between the proportion of temperatures within the recommended RDT storage conditions in the CCBs with water and the temperatures in the storage room (p < 0.001) and maximum temperatures were lower. RDTs stored at ambient temperatures were negative when tested with parasitized blood (2,000 parasites per micro litre) at 210 days, while the field RDTs kept in CCBs with water gave positive results until 360 days. The CCB was an effective tool for storage of RDTs at optimal conditions, and extended the effective life-span of the tests. The concept of evaporative cooling has potential to greatly enhance access to perishable diagnostics and medicines in remote communities, as it allows prolonged storage at low cost using locally-available materials, in the absence of electricity.

  1. Formation of ethyl carbamate and changes during fermentation and storage of yellow rice wine.

    PubMed

    Wu, Pinggu; Cai, Chenggang; Shen, Xianghong; Wang, Liyuan; Zhang, Jing; Tan, Ying; Jiang, Wei; Pan, Xiaodong

    2014-01-01

    Ethyl carbamate (EC) was analyzed during yellow rice wine production and storage. EC increased slowly during fermentation and rapidly after frying and sterilization. Less amount of EC was formed when cooled rapidly to 30 °C than when cooled naturally. High temperature and long storage time increased EC formation. After 400 days storage, EC increased from 74.0 to 84.2, 131.8 and 509.4 μg/kg at 4 °C, room temperature and 37 °C, respectively, and there was significantly difference between the fried wine and the wine on sale from 2011 (p<0.01). Urea increased during yellow rice wine fermentation and was above 20 mg/kg after the wine was fried; urea contributed to EC formation when the fried wine was cooled slowly. These results indicate that it is necessary for industry to optimize the wine frying conditions, such as temperature, time and cooling process in order to decrease EC formation. Copyright © 2013 Elsevier Ltd. All rights reserved.

  2. Maintaining high moisture content of macadamia nuts-in-shell during storage induces brown centres in raw kernels.

    PubMed

    Walton, David A; Randall, Bruce W; Le Lagadec, Marie D; Wallace, Helen M

    2013-09-01

    Kernel brown centres in macadamia are a defect causing internal discolouration of kernels. This study investigates the effect on the incidence of brown centres in raw kernel after maintaining high moisture content in macadamia nuts-in-shell stored at temperatures of 30°C, 35°C, 40°C and 45°C. Brown centres of raw kernel increased with nuts-in-shell storage time and temperature when high moisture content was maintained by sealing in polyethylene bags. Almost all kernels developed the defect when kept at high moisture content for 5 days at 45°C, and 44% developed brown centres after only 2 days of storage at high moisture content at 45°C. This contrasted with only 0.76% when stored for 2 days at 45°C but allowed to dry in open-mesh bags. At storage temperatures below 45°C, there were fewer brown centres, but there were still significant differences between those stored at high moisture content and those allowed to dry (P < 0.05). Maintenance of high moisture content during macadamia nuts-in-shell storage increases the incidence of brown centres in raw kernels and the defect increases with time and temperature. On-farm nuts-in-shell drying and storage practices should rapidly remove moisture to reduce losses. Ideally, nuts-in-shell should not be stored at high moisture content on-farm at temperatures over 30°C. © 2013 Society of Chemical Industry.

  3. Numerical simulations of the impact of seasonal heat storage on source zone emission in a TCE contaminated aquifer

    NASA Astrophysics Data System (ADS)

    Popp, Steffi; Beyer, Christof; Dahmke, Andreas; Bauer, Sebastian

    2016-04-01

    In urban regions, with high population densities and heat demand, seasonal high temperature heat storage in the shallow subsurface represents an attractive and efficient option for a sustainable heat supply. In fact, the major fraction of energy consumed in German households is used for room heating and hot water production. Especially in urbanized areas, however, the installation of high temperature heat storage systems is currently restricted due to concerns on negative influences on groundwater quality caused e.g. by possible interactions between heat storages and subsurface contaminants, which are a common problem in the urban subsurface. Detailed studies on the overall impact of the operation of high temperature heat storages on groundwater quality are scarce. Therefore, this work investigates possible interactions between groundwater temperature changes induced by heat storage via borehole heat exchangers and subsurface contaminations by numerical scenario analysis. For the simulation of non-isothermal groundwater flow, and reactive transport processes the OpenGeoSys code is used. A 2D horizontal cross section of a shallow groundwater aquifer is assumed in the simulated scenario, consisting of a sandy sediment typical for Northern Germany. Within the aquifer a residual trichloroethene (TCE) contaminant source zone is present. Temperature changes are induced by a seasonal heat storage placed within the aquifer with scenarios of maximum temperatures of 20°C, 40°C and 60°C, respectively, during heat injection and minimum temperatures of 2°C during heat extraction. In the scenario analysis also the location of the heat storage relative to the TCE source zone and plume was modified. Simulations were performed in a homogeneous aquifer as well as in a set of heterogeneous aquifers with hydraulic conductivity as spatially correlated random fields. In both cases, results show that the temperature increase in the heat plume and the consequential reduction of water viscosity lead to locally increased groundwater flow. Depending on the positioning of the heat storage relative to the TCE contamination, groundwater fluxes hence may be induced to increase within or partially bypass the TCE source zone. At the same time, TCE solubility decreases between 10 and 40 °C, which reduces TCE emission and almost compensates for the effects of a temperature induced increase of groundwater flow through the source zone. In total, the numerical simulations thus show only minor influences of the heat plume on the TCE emission compared to a thermally undisturbed aquifer. Acknowledgments: This work is part of the ANGUS+ project (www.angusplus.de) and funded by the German Federal Ministry of Education and Research (BMBF) as part of the energy storage initiative "Energiespeicher".

  4. Effect of Storage Temperature on Structure and Function of Cultured Human Oral Keratinocytes

    PubMed Central

    Islam, Rakibul; Jackson, Catherine; Eidet, Jon R.; Messelt, Edward B.; Corraya, Rima Maria; Lyberg, Torstein; Griffith, May; Dartt, Darlene A.; Utheim, Tor P.

    2015-01-01

    Purpose/Aims To assess the effect of storage temperature on the viability, phenotype, metabolism, and morphology of cultured human oral keratinocytes (HOK). Materials and Methods Cultured HOK cells were stored in HEPES- and sodium bicarbonate-buffered Minimum Essential Medium (MEM) at nine temperatures in approximately 4°C increments from 4°C to 37°C for seven days. Cells were characterized for viability by calcein fluorescence, phenotype retention by immunocytochemistry, metabolic parameters (pH, glucose, lactate, and O2) within the storage medium by blood gas analysis, and morphology by scanning electron microscopy and light microscopy. Results Relative to the cultured, but non-stored control cells, a high percentage of viable cells were retained only in the 12°C and 16°C storage groups (85%±13% and 68%±10%, respectively). Expression of ABCG2, Bmi1, C/EBPδ, PCNA, cytokeratin 18, and caspase-3 were preserved after storage in the 5 groups between 4°C and 20°C, compared to the non-stored control. Glucose, pH and pO2 in the storage medium declined, whereas lactate increased with increasing storage temperature. Morphology was best preserved following storage of the three groups between 12°C, 16°C, and 20°C. Conclusion We conclude that storage temperatures of 12°C and 16°C were optimal for maintenance of cell viability, phenotype, and morphology of cultured HOK. The storage method described in the present study may be applicable for other cell types and tissues; thus its significance may extend beyond HOK and the field of ophthalmology. PMID:26052937

  5. Effect of long term chilled (up to 5 weeks) then frozen (up to 12 months) storage at two different sub-zero holding temperatures on beef: 3. Protein structure degradation and a marker of protein oxidation.

    PubMed

    Holman, Benjamin W B; Coombs, Cassius E O; Morris, Stephen; Kerr, Matthew J; Hopkins, David L

    2018-05-01

    Different chilled (~0.1 °C for up to 5 weeks) then frozen storage (up to 12 months) combinations and two frozen storage holding temperatures (-12 °C and -18 °C) effects on beef M. longissimus lumborum (LL) protein structure degradation and a marker of protein oxidation were tested. Particle size (PS) analysis and protein solubility results found storage combination effects on protein degradation to be significant (P < 0.05), although the influence of frozen holding temperatures was negligible. LL carbonyl, and nitrate and nitrite content responses were variable and yet broadly reflected an increased incidence of protein oxidation across increasing chilled storage and ensuing frozen storage periods - this aspect meriting future exploration. Total myoglobin content and the estimated myoglobin redox fractions (metmyoglobin, deoxymyoglobin, and oxymyoglobin) were also subject to storage treatment. These findings demonstrate the capacity for beef storage selection (chilled-then-frozen) to manage compositional protein changes and its implications on sensory quality traits across comparative 'long term' durations. Crown Copyright © 2018. Published by Elsevier Ltd. All rights reserved.

  6. Influence of steeping solution and storage temperature on the color change of garlic.

    PubMed

    Bae, Song Hwan; Lee, Seog-Won; Kim, Mi-Ryung; Kim, Jin Man; Suh, Hyung Joo

    2010-01-01

    The objective of this study was to investigate the browning of garlic under different steeping conditions and storage temperatures. The brown indices of steeped garlics showed lowest values (7.3 and 7) in 25% and 50% EtOH at 7 d of storage. The degree of browning of steeped garlics was lowest (10.2 in 25% EtOH and 10.4 in 50% EtOH) in the samples soaked for 8 h at 13 d of storage. As the storage temperature was increased from 10 to 40 degrees C, the brown indices of garlics revealed an increasing trend relative to storage time regardless of steeping treatment. Overall, the kinetic parameters showed relatively low R(2) and irregular reaction constants, but the k(o) values showed an increasing trend with temperature under a zero-order model. The highest polyphenol content within the garlic bulbs was seen in controls (without steeping treatment, 588.9 microg/g), than 0% EtOH (water, 392.5 microg/g), than 25% EtOH (211.3 microg/g), and finally 50% EtOH (155.6 microg/g). The polyphenol oxidase activity of garlic showed a similar trend to that of polyphenol content. However, the texture properties of garlics steeped with 25% and 50% did not change. The garlic color preferred by consumers is a creamy-white, but this is susceptible to enzymatic browning when pre-peeled and chopped. When garlic was steeped in the 25% and 50% alcohol, the browning of garlic was prevented during storage.

  7. Rheological and morphological characterizations on physical stability of gamma-oryzanol-loaded solid lipid nanoparticles (SLNs).

    PubMed

    Seetapan, Nispa; Bejrapha, Piyawan; Srinuanchai, Wanwisa; Ruktanonchai, Uracha Rungsardthong

    2010-01-01

    In the present study, gamma-oryzanol was incorporated into glycerol behenate (Compritol 888 ATO) nanoparticles (SLNs) at 5 and 10% (w/w) of lipid phase. Increasing lipid phase concentration resulted in increased consistency and particle diameter of SLNs. Upon storage over 60 days at 4, 25 and 40 degrees C, the instability was observed by rheological analysis for all samples due to the formation of gelation. Rheological measurement revealed the increase in storage modulus and critical stress during storage at all temperatures. However, at 40 degrees C, the pronounced instability was observed from the highest increase in storage modulus and a formation of rod-like network structure from scanning electron micrographs. An increase in crystallinity, determined by differential scanning calorimetry, was also found during storage at all temperatures, confirming the instability of SLNs. Particle diameters and zeta potentials of both concentrations at all storage conditions failed to explain the observed instability. These investigations may help to develop formulations of solid lipid nanoparticles, which are optimized with respect to the desired rheological properties.

  8. Physicochemical Changes and Resistant-Starch Content of Extruded Cornstarch with and without Storage at Refrigerator Temperatures.

    PubMed

    Neder-Suárez, David; Amaya-Guerra, Carlos A; Quintero-Ramos, Armando; Pérez-Carrillo, Esther; Alanís-Guzmán, María G de J; Báez-González, Juan G; García-Díaz, Carlos L; Núñez-González, María A; Lardizábal-Gutiérrez, Daniel; Jiménez-Castro, Jorge A

    2016-08-15

    Effects of extrusion cooking and low-temperature storage on the physicochemical changes and resistant starch (RS) content in cornstarch were evaluated. The cornstarch was conditioned at 20%-40% moisture contents and extruded in the range 90-130 °C and at screw speeds in the range 200-360 rpm. The extrudates were stored at 4 °C for 120 h and then at room temperature. The water absorption, solubility index, RS content, viscoelastic, thermal, and microstructural properties of the extrudates were evaluated before and after storage. The extrusion temperature and moisture content significantly affected the physicochemical properties of the extrudates before and after storage. The RS content increased with increasing moisture content and extrusion temperature, and the viscoelastic and thermal properties showed related behaviors. Microscopic analysis showed that extrusion cooking damaged the native starch structure, producing gelatinization and retrogradation and forming RS. The starch containing 35% moisture and extruded at 120 °C and 320 rpm produced the most RS (1.13 g/100 g) after to storage at low temperature. Although the RS formation was low, the results suggest that extrusion cooking could be advantageous for RS production and application in the food industry since it is a pollution less, continuous process requiring only a short residence time.

  9. FRAPCON analysis of cladding performance during dry storage operations

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Richmond, David J.; Geelhood, Kenneth J.

    There is an increasing need in the U.S. and around the world to move used nuclear fuel from wet storage in fuel pools to dry storage in casks stored at independent spent fuel storage installations (ISFSI) or interim storage sites. The NRC limits cladding temperature to 400°C while maintaining cladding hoop stress below 90 MPa in an effort to avoid radial hydride reorientation. An analysis was conducted with FRAPCON-4.0 on three modern fuel designs with three representative used nuclear fuel storage temperature profiles that peaked at 400 °C. Results were representative of the majority of U.S. LWR fuel. They conservativelymore » showed that hoop stress remains below 90 MPa at the licensing temperature limit. Results also show that the limiting case for hoop stress may not be at the highest rod internal pressure in all cases but will be related to the axial temperature and oxidation profiles of the rods at the end of life and in storage.« less

  10. Effect of packaging and storage temperature on the survival of Listeria monocytogenes inoculated postprocessing on sliced salami.

    PubMed

    Gounadaki, Antonia S; Skandamis, Panagiotis N; Drosinos, Eleftherios H; Nychas, George-John E

    2007-10-01

    The survival of postprocess Listeria monocytogenes contamination on sliced salami, stored under the temperatures associated with retail and domestic storage, was investigated. Sliced salami was inoculated with low and high concentrations of L. monocytogenes before being packaged under vacuum or air. Survival of L. monocytogenes was determined after storage of sausages for 45 or 90 days for low or high sample inocula, respectively, at 5, 15, and 25 degrees C. All survival curves of L. monocytogenes were characterized by an initial rapid inactivation within the first days of storage, followed by a second, slower inactivation phase or "tailing." Greater reduction of L. monocytogenes was observed at the high storage temperature (25 degrees C), followed by ambient (15 degrees C) and chill (5 degrees C) storage conditions. Moreover, vacuum packaging resulted in a slower destruction of L. monocytogenes than air packaging, and this effect increased as storage temperature decreased. Although L. monocytogenes numbers decreased to undetectable levels by the end of the storage period, the time (in days) needed for this reduction and for the total elimination of the pathogen decreased with high temperature, aerobic storage, and high inoculum. Results of this study clearly indicated that the kinetics of L. monocytogenes were highly dependent on the interaction of factors such as storage temperature, packaging conditions, and initial level of contamination (inoculum). These results may contribute to the exposure assessment of quantitative microbial risk assessment and to the establishment of storage-packaging recommendations of fermented sausages.

  11. Temperature fluctuations during deep temperature cryopreservation reduce PBMC recovery, viability and T-cell function.

    PubMed

    Germann, Anja; Oh, Young-Joo; Schmidt, Tomm; Schön, Uwe; Zimmermann, Heiko; von Briesen, Hagen

    2013-10-01

    The ability to analyze cryopreserved peripheral blood mononuclear cell (PBMC) from biobanks for antigen-specific immunity is necessary to evaluate response to immune-based therapies. To ensure comparable assay results, collaborative research in multicenter trials needs reliable and reproducible cryopreservation that maintains cell viability and functionality. A standardized cryopreservation procedure is comprised of not only sample collection, preparation and freezing but also low temperature storage in liquid nitrogen without any temperature fluctuations, to avoid cell damage. Therefore, we have developed a storage approach to minimize suboptimal storage conditions in order to maximize cell viability, recovery and T-cell functionality. We compared the influence of repeated temperature fluctuations on cell health from sample storage, sample sorting and removal in comparison to sample storage without temperature rises. We found that cyclical temperature shifts during low temperature storage reduce cell viability, recovery and immune response against specific-antigens. We showed that samples handled under a protective hood system, to avoid or minimize such repeated temperature rises, have comparable cell viability and cell recovery rates to samples stored without any temperature fluctuations. Also T-cell functionality could be considerably increased with the use of the protective hood system compared to sample handling without such a protection system. This data suggests that the impact of temperature fluctuation on cell integrity should be carefully considered in future clinical vaccine trials and consideration should be given to optimal sample storage conditions. Copyright © 2013 The Authors. Published by Elsevier Inc. All rights reserved.

  12. Effect of K loadings on nitrate formation/decomposition and on NOx storage performance of K-based NOx storage-reduction catalysts

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Kim, Do Heui; Mudiyanselage, Kumudu K.; Szanyi, Janos

    2013-10-25

    We have investigated nitrate formation and decomposition processes, and measured NOx storage performance on Pt-K2O/Al2O3 catalysts as a function of potassium loading. After NO2 adsorption at room temperature, ionic and bidentate nitrates were observed by fourier transform infra-red (FTIR) spectroscopy. The ratio of the former to the latter species increased with increasing potassium loading up to 10 wt%, and then stayed almost constant with additional K, demonstrating a clear dependence of loading on the morphology of the K species. Although both K2O(10)/Al2O3 and K2O(20)/Al2O3 samples have similar nitrate species after NO2 adsorption, the latter has more thermally stable nitrate speciesmore » as evidenced by FTIR and NO2 temperature programmed desorption (TPD) results. With regard to NOx storage performance, the temperature of maximum NOx uptake (Tmax) is 573 K up to a potassium loading of 10 wt%. As the potassium loading increases from 10 wt% to 20 wt%, Tmax shifted from 573 K to 723 K. Moreover, the amount of NO uptake (38 cm3 NOx/g catal) at Tmax increased more than three times, indicating that efficiency of K in storing NOx is enhanced significantly at higher temperature, in good agreement with the NO2 TPD and FTIR results. Thus, a combination of characterization and NOx storage performance results demonstrates an unexpected effect of potassium loading on nitrate formation and decomposition processes; results important for developing Pt-K2O/Al2O3 for potential applications as high temperature NOx storage-reduction catalysts.« less

  13. Impacts of convection on high-temperature aquifer thermal energy storage

    NASA Astrophysics Data System (ADS)

    Beyer, Christof; Hintze, Meike; Bauer, Sebastian

    2016-04-01

    Seasonal subsurface heat storage is increasingly used in order to overcome the temporal disparities between heat production from renewable sources like solar thermal installations or from industrial surplus heat and the heat demand for building climatisation or hot water supply. In this context, high-temperature aquifer thermal energy storage (ATES) is a technology to efficiently store and retrieve large amounts of heat using groundwater wells in an aquifer to inject or withdraw hot or cold water. Depending on the local hydrogeology and temperature amplitudes during high-temperature ATES, density differences between the injected hot water and the ambient groundwater may induce significant convective flow components in the groundwater flow field. As a consequence, stored heat may accumulate at the top of the storage aquifer which reduces the heat recovery efficiency of the ATES system. Also, an accumulation of heat at the aquifer top will induce increased emissions of heat to overlying formations with potential impacts on groundwater quality outside of the storage. This work investigates the impacts of convective heat transport on the storage efficiency of a hypothetical high-temperature ATES system for seasonal heat storage as well as heat emissions to neighboring formations by numerical scenario simulations. The coupled groundwater flow and heat transport code OpenGeoSys is used to simulate a medium scale ATES system operating in a sandy aquifer of 20 m thickness with an average groundwater temperature of 10°C and confining aquicludes at top and bottom. Seasonal heat storage by a well doublet (i.e. one fully screened "hot" and "cold" well, respectively) is simulated over a period of 10 years with biannual injection / withdrawal cycles at pumping rates of 15 m³/h and for different scenarios of the temperature of the injected water (20, 35, 60 and 90 °C). Simulation results show, that for the simulated system significant convective heat transport sets in when injection temperatures exceed 35°C. Convection results in an accumulation of heat below the upper confining layer. The consequential increase of the heat plume contact area with this formation results in increased conductive heat transfer. Also, thermal gradients between the heat plume and the ambient groundwater increase with injection temperature, which increases heat conduction within the aquifer. Both effects reduce the thermal recovery of the ATES system. At the end of the 10th injection / withdrawal cycle the efficiency of thermal recovery thus reaches about 76 % for the 20°C scenario, 74% for 35°C, 71 % for 60°C and 66 % for the 90 °C scenario. Sensitivity analysis indicates that permeability in horizontal and vertical directions are controlling factors for the extent of convective heat displacement. Also, heat plume dimensions are influenced by permeability, and to a lesser extent by heat capacity and porosity of the aquifer. The planning of high-temperature ATES at a specific site hence requires a careful investigation of hydraulic and heat transport properties. Acknowledgments: This work is part of the ANGUS+ project (www.angusplus.de) and funded by the German Federal Ministry of Education and Research (BMBF) as part of the energy storage initiative "Energiespeicher".

  14. Comparison of Microbial Communities in Swine Manure at Various Temperatures and storage times.

    PubMed

    Lim, Joung-Soo; Yang, Seung Hak; Kim, Bong-Soo; Lee, Eun Young

    2018-01-26

    This study was designed to investigate the effects of temperature and storage time on the evolution of bacterial communities in swine manure. Manure was stored at -20°C, 4°C, 20°C, or 37°C and sampled at 7-day intervals over 28 days of storage, for a total of 5 time points. To assess the bacterial species present, 16S ribosomal RNA gene sequences were analyzed using pyrosequencing. After normalization, 113,934 sequence reads were obtained, with an average length of 466.6 ± 4.4 bp. The diversity indices of the communities reduced as temperature and storage time increased, and the slopes of rarefaction curves decreased from the second week in samples stored at -20 °C and 4 °C. These results indicate that the richness of the bacterial community in the manure reduced as temperature and storage time increased. Firmicutes were the dominant phylum in all samples examined, ranging from 89.3% to 98.8% of total reads, followed by Actinobacteria, which accounted for 0.6% to 7.9%. A change in community composition was observed in samples stored at 37 °C during the first 7 days, indicating that temperature plays an important role in determining the microbiota of swine manure. Clostridium, Turicibacter, Streptococcus, and Lactobacillus within Firmicutes, and Corynebacterium within Actinobacteria were the most dominant genera in fresh manure and all stored samples. Based on our findings, we propose Clostridium as an indicator genus of swine manure decomposition in an anaerobic environment. The proportions of dominant genera changed in samples stored at 20 °C and 37 °C during the fourth week. Based on these results, it was concluded that the microbial communities of swine manure change rapidly as storage time and temperature increase.

  15. Effect of cobalt-60 gamma rays on the storage behaviour of garlic bulbs at room temperature and in cold storage

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Habibunnisa; Mathur, P.B.; Bano, Z.

    1971-11-01

    Effect of cobalt-60 gamma rays at a dose-rate of 6 krad on the storage behavior of garlic bulbs packaged individually and in lots of eight in perforated polyethylene bags of 200 gauge was investigated at room temperature (75 to 90 deg F) and cold temperature (32 to 35 deg F) under relative humidity 85 to 90%. Irradiation was immediately followed by an increase in the rate of respiration in the garlic bulbs followed by a decrease in the rate of respiration towards the later part of the storage period. At room temperature, sprouting was inhibited to a considerable extent, whilemore » in cold storage after a storage period of 9 months sprouting was completely prevented. The percentage sprouting was more in large size garlic bulbs than in small sized ones. For extension of storage life, packaging singly in polyethylene bags, selection of small sized garlic bulbs, storage at 32 to 35 deg F and irradiation with 6 krad of cobalt 60 gamma rays are recommended. (INIS)« less

  16. Use of palm mid-fraction in dark chocolate as base filling centre at different storage temperatures.

    PubMed

    Jinap, S; Ali, A A; Man, Y B; Suria, A M

    2000-11-01

    Dark chocolates filled with palm mid-fraction (PMF) were stored at different temperatures to evaluate the physical and chemical changes. Storage at low temperature (18 degrees C) reduces the PMF migration to negligible extent. Higher storage temperatures (30 and 35 degrees C) increased the PMF migration from the filling centre into the chocolate coating. As a consequence of fat migration, fatty acid composition, triglyceride composition, hardness, solid fat content, melting point and polymorphic structure changed, leading to bloom formation, which started by fat migration and was influenced by recrystallization tendency within the chocolate coating.

  17. Effect of storage and drying temperature on the gelation behavior and structural characteristics of sericin.

    PubMed

    Jo, Yoon Nam; Park, Byung-Dae; Um, In Chul

    2015-11-01

    Owing to unique properties, including the wound healing effect, sericin gel and films have attracted significant attention in the biomedical and cosmetic fields. The structural characteristics and properties of sericin gels and films are especially important owing to their effect on the performance of sericin in biomedical and cosmetic applications. In the present study, the effect of temperature on the gelation behavior, gel disruption, and sol-gel transition of sericin was examined using rheometry. In addition, the effect of the drying temperature on the structural characteristics of the sericin film was determined via Fourier transform infrared (FTIR) spectroscopy. The strength of the sericin gel increased and the gelation process was prolonged with decreasing storage temperatures. FTIR and differential scanning calorimetry (DSC) results also revealed that the crystallinity and the thermal decomposition temperature of the sericin film increased with decreasing drying temperature. The sericin gels were disrupted at a storage time of 40min when they were stored at temperatures higher than 50°C, and the corresponding gel strength decreased with increasing temperature. Furthermore, the thermo-reversible nature of gel-sol transition of sericin was confirmed by rheological and FTIR measurements. Copyright © 2015 Elsevier B.V. All rights reserved.

  18. Role of invertase activity in processing quality of potatoes: Effect of storage temperature and duration.

    PubMed

    Bandana; Sharma, Vineet; Singh, Brajesh; Raigond, Pinky; Kaushik, S K

    2016-03-01

    Invertase activity and processing attributes of three potato cultivars were studied to find the reason for deterioration of processing quality during their prolonged storage in commercial cold stores (4°C) as compared to elevated temperature storage (12 ± 0.5°C), with CIPC {Isopropyl-N-(3-Cholorophenyl) carbamate}. Lower storage temperature (4°C) tended to be more effective in increasing invertase activity of potato tubers than elevated temperature. Non-processing cultivar viz., Kufri Pukhraj resulted in accumulation of more invertase activity than relatively two processing cultivars. Kufri Chipsona-1 and Kufri Chipsona-3 at 12 ± 0.5°C possessed basal invertase activity ranging from 39.3 to 79.8 and 54.1 to 93.8 (pmoles hexose h⁻¹ g⁻¹ f.wt.) respectively, during two years. Total invertase activity at 4°C increased abruptly and remained high from 30 to 60 days of storage. The activity progressively reached 90.6 to 106.6 and 81.4 to 101.3 during both the years respectively, after 60 days of storage to that observed initially. Reducing sugar content increased from 23.3 to 105.7 and 389.0 to 1138.2 (mg 100g⁻¹ f.wt.) after 90 days of storage at 12 ± 0.5°C and 4°C, respectively. Studies concluded that basal and total invertase, were responsible for cold-induced sweetening and resulted in deterioration of processing quality of potatoes during storage at 4°C. Since this activity is low at 12 ± 0.5°C, the processing traits remained acceptable to industry and consumers.

  19. The Effect of Storage Condition and Duration on the Deterioration of Primed Rice Seeds

    PubMed Central

    Wang, Weiqin; He, Aibin; Peng, Shaobing; Huang, Jianliang; Cui, Kehui; Nie, Lixiao

    2018-01-01

    Seed priming is a successful practice to improve crop establishment under adverse environment. However, reduced longevity of primed rice (Oryza sativa L.) seeds during storage limited the adoption of this technique. Present study investigated the effect of temperature, relative air humidity (RH) and oxygen on the longevity of primed rice seeds in a range of 60 days storage. In addition, the biochemical and morphological mechanisms associated with deterioration of primed seeds during storage were explored. Three types of priming treated rice seeds and one non-primed control were stored under (1) low temperature-vacuum (LT-V), (2) room temperature-vacuum (RT-V), (3) room temperature-aerobic-low RH (RT-A-LH) and (4) room temperature-aerobic- high RH (RT-A-HH) for 0, 15, 30, 45, and 60 days. The results showed that storage of seeds under different conditions for 15–60 days did not influence the longevity of non-primed rice seeds. Meanwhile, the viability of primed rice seeds did not reduce when stored under LT-V, RT-V, and RT-A-LH, but was significantly reduced under RT-A-HH. Under vacuum condition, the increases of storage temperature (30°C) did not reduce the longevity of primed seeds. Likewise, the oxygen did not influence the longevity of primed rice seeds stored under low RH. Nevertheless, increase of RH significantly reduced the viability of primed seeds stored for 15–60 days. Reduced starch metabolism, the consumption of starch reserves in rice endosperms, the accumulation of malondialdehyde and the decreases of antioxidant enzyme activities might be associated with the deterioration of primed rice seeds during storage. In conclusion, storage of primed seeds under high RH condition beyond 15 days is deteriorative for germination and growth of rice. The primed rice seeds are recommended to store at vacuum or low RH or low temperature condition to ensure good crop establishment. PMID:29487612

  20. The Effect of Storage Condition and Duration on the Deterioration of Primed Rice Seeds.

    PubMed

    Wang, Weiqin; He, Aibin; Peng, Shaobing; Huang, Jianliang; Cui, Kehui; Nie, Lixiao

    2018-01-01

    Seed priming is a successful practice to improve crop establishment under adverse environment. However, reduced longevity of primed rice ( Oryza sativa L.) seeds during storage limited the adoption of this technique. Present study investigated the effect of temperature, relative air humidity (RH) and oxygen on the longevity of primed rice seeds in a range of 60 days storage. In addition, the biochemical and morphological mechanisms associated with deterioration of primed seeds during storage were explored. Three types of priming treated rice seeds and one non-primed control were stored under (1) low temperature-vacuum (LT-V), (2) room temperature-vacuum (RT-V), (3) room temperature-aerobic-low RH (RT-A-LH) and (4) room temperature-aerobic- high RH (RT-A-HH) for 0, 15, 30, 45, and 60 days. The results showed that storage of seeds under different conditions for 15-60 days did not influence the longevity of non-primed rice seeds. Meanwhile, the viability of primed rice seeds did not reduce when stored under LT-V, RT-V, and RT-A-LH, but was significantly reduced under RT-A-HH. Under vacuum condition, the increases of storage temperature (30°C) did not reduce the longevity of primed seeds. Likewise, the oxygen did not influence the longevity of primed rice seeds stored under low RH. Nevertheless, increase of RH significantly reduced the viability of primed seeds stored for 15-60 days. Reduced starch metabolism, the consumption of starch reserves in rice endosperms, the accumulation of malondialdehyde and the decreases of antioxidant enzyme activities might be associated with the deterioration of primed rice seeds during storage. In conclusion, storage of primed seeds under high RH condition beyond 15 days is deteriorative for germination and growth of rice. The primed rice seeds are recommended to store at vacuum or low RH or low temperature condition to ensure good crop establishment.

  1. [Study on content of ethyl carbamate in yellow rice wine and its change in Zhejiang province].

    PubMed

    Shen, Xiang-hong; Wu, Ping-gu; Wang, Li-yuan; Zhang, Jing; Tan, Ying; Ying, Ying; Ma, Bing-jie

    2013-11-01

    To understand the content status of ethyl carbamate (EC) in yellow rice wine and the changes in storage period and shelf life in Zhejiang province. A total of 475 samples of yellow rice wine purchased randomly from supermarkets and food stores in Zhejiang province during 2008-2012, and 49 samples collected from manufacturers were measured for EC content. The sample collected from manufacturers by filter sterilization was placed at 4 °C, room temperature and 37 °C for 400 d, respectively;a bottled wine and a wine in bag were bought from market were placed for 400 d in room temperature to conduct shelf life storage test, and measure the content in every point in 2011. The EC of the samples was determinated by gas chromatography-mass spectrometry after the samples were diluted with D5-EC isotope dilution technique, and purified by alkaline diatomite solid phase extraction column. The overall detection rate of EC was 99% (472/475) in yellow rice wine of Zhejiang province in 2008-2012, the median value was 70-112 µg/kg, the 90th percentile was 190-333 µg/kg, the 95th percentile was 214-393 µg/kg, and the maximum value was 430-515 µg/kg. The content of EC was increased gradually along with the increasing of storage age in commercially yellow rice wine, and the average content of EC were positively correlated with storage age(r = 0.988). The contents of EC in yellow rice wine after sterilization increased from 74 µg/kg to 86 µg/kg, 127 µg/kg and 509 µg/kg at 4 °C, room temperature and 37°C, respectively for 400 d storage, the differences had statistical significance (F = 14.73, P < 0.01). The content of EC in yellow rice wines in shelf life, which stored in room temperature with bottle and bag package, was decreased slightly with increasing storage time in the beginning, from 215 to 184 µg/kg and 196 to 158 µg/kg, respectively, and increased again with increasing storage time after 250 d, with 252 µg/kg and 210 µg/kg in bottle and bag package after 400 d, respectively, the differences had statistical significance (Z = 2.37, P < 0.05). EC is widespread in rice wine, the content of EC was correlated with storage time and temperature.

  2. Influence of critical storage temperatures on degradative pathways of pigments in green beans (Phaseolus vulgaris cvs. Perona and boby).

    PubMed

    Monreal, M; De Ancos, B; Cano, M P

    1999-01-01

    In this work a study of critical storage temperatures on pigment degradation of green beans (Phaseolus vulgaris, cvs. Perona and Boby) was conducted. In this way, green beans kept better quality at 4 degrees C than either 8 or 12 degrees C, maintaining a bright green color and good texture. Nevertheless, temperatures of 4 degrees C induced chilling injury (CI) after eight days of storage, which became evident when the pods were transferred to 20 degrees C. Cold storage temperatures, 12, 8, and 4 degrees C, produced different changes on the green beans chlorophyll profile. Green beans of both cultivars, Perona and Boby, stored at 4 and 12 degrees C showed a continuous degradation of chlorophyll pigments during storage, while samples stored at 8 degrees C showed an increase of chlorophyll content at the first 15 days. Carotenoid pigments also suffered different changes during cold storage. Perona was the green beans cultivar which maintained the higher level of lutein, mainly when samples were stored at the most suitable temperature (8 degrees C).

  3. Metabolic Responses to Low Temperature of Three Peach Fruit Cultivars Differently Sensitive to Cold Storage

    PubMed Central

    Brizzolara, Stefano; Hertog, Maarten; Tosetti, Roberta; Nicolai, Bart; Tonutti, Pietro

    2018-01-01

    Refrigerated storage is widely applied in order to maintain peach quality but it can also induce chilling injuries (CIs) such as flesh browning and bleeding, and mealiness. Peach fruit from three cultivars (‘Red Haven’, RH, ‘Regina di Londa’, RL, and ‘Flaminia’, FL) were stored for 4 weeks under low temperatures (0.5 and 5.5°C). GC-MS was employed to study changes in both metabolome and volatilome induced by cold storage in the mesocarp. CIs were assessed both at the end of each week of storage and after subsequent shelf-life (SL) at 20°C. Flesh browning and mealiness appeared to be more related to 5.5°C storage, while flesh bleeding revealed high incidence following 0.5°C storage. Compared to RL and FL, RH showed a marked lower incidence of CIs. Multivariate statistical analyses indicate that RH peaches indeed differ from RL and FL in particular when considering data from samples collected at the end of the cold storage. Common and divergent responses have been identified in terms of metabolic responses to the applied low temperatures. In all three cultivars raffinose, glucose-6P, fucose, xylose, sorbitol, GABA, epicatechin, catechin, and putrescine markedly increased during cold storage, while citramalic, glucuronic, mucic and shikimic acids decreased. Among volatile organic compounds (VOCs), aldehydes and alcohols generally accumulated more under low temperature conditions while esters and lactones evolved during subsequent SL. The main cultivar differences developed after cold storage during SL although some common responses (e.g., an increased production of ethyl acetate) were observed. The lower levels of flesh browning and bleeding displayed by RH peaches were related to compounds with antioxidant activity, or acting as osmotic protectants and membrane stabilizer. Indeed, RH showed higher levels of amino acids and urea, together with a marked increase in putrescine, sorbitol, maltitol, myoinositol and sucrose detected during storage and SL. PMID:29892309

  4. Metabolic Responses to Low Temperature of Three Peach Fruit Cultivars Differently Sensitive to Cold Storage.

    PubMed

    Brizzolara, Stefano; Hertog, Maarten; Tosetti, Roberta; Nicolai, Bart; Tonutti, Pietro

    2018-01-01

    Refrigerated storage is widely applied in order to maintain peach quality but it can also induce chilling injuries (CIs) such as flesh browning and bleeding, and mealiness. Peach fruit from three cultivars ('Red Haven', RH, 'Regina di Londa', RL, and 'Flaminia', FL) were stored for 4 weeks under low temperatures (0.5 and 5.5°C). GC-MS was employed to study changes in both metabolome and volatilome induced by cold storage in the mesocarp. CIs were assessed both at the end of each week of storage and after subsequent shelf-life (SL) at 20°C. Flesh browning and mealiness appeared to be more related to 5.5°C storage, while flesh bleeding revealed high incidence following 0.5°C storage. Compared to RL and FL, RH showed a marked lower incidence of CIs. Multivariate statistical analyses indicate that RH peaches indeed differ from RL and FL in particular when considering data from samples collected at the end of the cold storage. Common and divergent responses have been identified in terms of metabolic responses to the applied low temperatures. In all three cultivars raffinose, glucose-6P, fucose, xylose, sorbitol, GABA, epicatechin, catechin, and putrescine markedly increased during cold storage, while citramalic, glucuronic, mucic and shikimic acids decreased. Among volatile organic compounds (VOCs), aldehydes and alcohols generally accumulated more under low temperature conditions while esters and lactones evolved during subsequent SL. The main cultivar differences developed after cold storage during SL although some common responses (e.g., an increased production of ethyl acetate) were observed. The lower levels of flesh browning and bleeding displayed by RH peaches were related to compounds with antioxidant activity, or acting as osmotic protectants and membrane stabilizer. Indeed, RH showed higher levels of amino acids and urea, together with a marked increase in putrescine, sorbitol, maltitol, myoinositol and sucrose detected during storage and SL.

  5. Cyclic high temperature heat storage using borehole heat exchangers

    NASA Astrophysics Data System (ADS)

    Boockmeyer, Anke; Delfs, Jens-Olaf; Bauer, Sebastian

    2016-04-01

    The transition of the German energy supply towards mainly renewable energy sources like wind or solar power, termed "Energiewende", makes energy storage a requirement in order to compensate their fluctuating production and to ensure a reliable energy and power supply. One option is to store heat in the subsurface using borehole heat exchangers (BHEs). Efficiency of thermal storage is increasing with increasing temperatures, as heat at high temperatures is more easily injected and extracted than at temperatures at ambient levels. This work aims at quantifying achievable storage capacities, storage cycle times, injection and extraction rates as well as thermal and hydraulic effects induced in the subsurface for a BHE storage site in the shallow subsurface. To achieve these aims, simulation of these highly dynamic storage sites is performed. A detailed, high-resolution numerical simulation model was developed, that accounts for all BHE components in geometrical detail and incorporates the governing processes. This model was verified using high quality experimental data and is shown to achieve accurate simulation results with excellent fit to the available experimental data, but also leads to large computational times due to the large numerical meshes required for discretizing the highly transient effects. An approximate numerical model for each type of BHE (single U, double U and coaxial) that reduces the number of elements and the simulation time significantly was therefore developed for use in larger scale simulations. The approximate numerical model still includes all BHE components and represents the temporal and spatial temperature distribution with a deviation of less than 2% from the fully discretized model. Simulation times are reduced by a factor of ~10 for single U-tube BHEs, ~20 for double U-tube BHEs and ~150 for coaxial BHEs. This model is then used to investigate achievable storage capacity, injection and extraction rates as well as induced effects for varying storage cycle times, operating conditions and storage set-ups. A sensitivity analysis shows that storage efficiency strongly depends on the number of BHEs composing the storage site and the cycle time. Using a half-yearly cycle of heat injection and extraction with the maximum possible rates shows that the fraction of recovered heat increases with the number of storage cycles used, as initial losses due to heat conduction become smaller. Also, overall recovery rates of 70 to 80% are possible in the set-ups investigated. Temperature distribution in the geological heat storage site is most sensitive to the thermal conductivity of both borehole grouting and storage formation, while storage efficiency is dominated by the thermal conductivity of the storage formation. For the large cycle times of 6 months each used, heat capacity is less sensitive than the heat conductivity. Acknowledgments: This work is part of the ANGUS+ project (www.angusplus.de) and funded by the German Federal Ministry of Education and Research (BMBF) as part of the energy storage initiative "Energiespeicher".

  6. Shifts in spinach microbial communities after chlorine washing and storage at compliant and abusive temperatures.

    PubMed

    Gu, Ganyu; Ottesen, Andrea; Bolten, Samantha; Ramachandran, Padmini; Reed, Elizabeth; Rideout, Steve; Luo, Yaguang; Patel, Jitendra; Brown, Eric; Nou, Xiangwu

    2018-08-01

    Fresh produce, like spinach, harbors diverse bacterial populations, including spoilage and potentially pathogenic bacteria. This study examined the effects of produce washing in chlorinated water and subsequent storage on the microbiota of spinach. Baby spinach leaves from a commercial fresh-cut produce processor were assessed before and after washing in chlorinated water, and then after one week's storage at 4, 10, and 15 °C. Microbial communities on spinach were analyzed by non-selective plating, qPCR, and 16S rDNA amplicon sequencing. Bacterial populations on spinach, averaging 6.12 ± 0.61 log CFU/g, were reduced by 1.33 ± 0.57 log after washing. However, populations increased by 1.77-3.24 log after storage, with larger increases occurring at higher temperature (15 > 10 > 4 °C). The predominant phylum identified on unwashed spinach leaves was Proteobacteria; dominant genera were Pseudomonas and Sphingomonas. Bacterial communities shifted significantly after chlorine washing and storage. Several Proteobacteria species, such as Stenotrophomonas sp. and Erwinia sp., were relatively tolerant of chlorine treatment, while species of Flavobacterium and Pedobacter (phylum Bacteroidetes) grew rapidly during storage, especially at abusive temperatures. Cupriavidus sp. and Ralstonia sp. showed significant increases after washing. After storage, microbial communities on spinach appeared to shift back toward the pre-washing distributions. Copyright © 2018. Published by Elsevier Ltd.

  7. The impact of home freezing on the sensory characteristics of ready-to-use leafy vegetables.

    PubMed

    Mohammadi, Mehrdad; Koushki, Mohammad Reza; Ahmadian, Fariba Seyed; Moslemy, Masoumeh

    2011-02-01

    Owing to the increasing trend of consumption of ready-to-use leafy vegetables, the necessity of determining the best conditions for their frozen storage and the considerable impact of freezing on their sensory attributes, research was carried out to determine the best freezing temperature and storage time for a mixture of Allium ampeloprasum, Lepidium sativum and Stureia hortensis. The results for freezing temperature at three different storage times showed that colour and overall acceptability at - 18 °C were always ranked first (P < 0.05), while taste at - 18 °C was ranked first on days 120 and 150. The results for frozen storage time at three different temperatures indicated that colour, taste and acceptability were not significantly different. Overall, the results of this research indicated that the sensory attributes of leafy vegetables during 180 days of frozen storage were affected mainly by freezing temperature rather than frozen storage time. 2010 Society of Chemical Industry.

  8. Exhaled nitric oxide in mylar balloons: influence of storage time, humidity and temperature.

    PubMed Central

    Bodini, Alessandro; Pijnenburg, Mariëlle W H; Boner, Atillio L; de Jongste, Johan C

    2003-01-01

    BACKGROUND: Mylar balloons are used to collect exhaled air for analysis of fractional nitric oxide concentration (FENO). AIM: We studied the effect of storage conditions on the stability of nitric oxide (NO) in mylar balloons. METHODS: Exhaled air samples and calibration gases were stored in mylar balloons at 4, 21 and 37 degrees C, with or without silica gel. NO was measured after 0, 6, 9, 24 and 48 h. Scheffe F-tests were used to compare NO values. RESULTS: NO remained stable in balloons for 9 h at all temperatures, without silica gel. NO increased between 9 and 48 h, but only with low initial FENO. Silica gel increased variability. CONCLUSIONS: FENO in mylar balloons is stable for at least 9 h. The storage temperature is not critical, but silica gel increases variability. PMID:12745548

  9. Oxidative deterioration of pork during superchilling storage.

    PubMed

    Pomponio, Luigi; Ruiz-Carrascal, Jorge

    2017-12-01

    In superchilling (SC), meat is kept at temperatures around 1 °C below its initial freezing point, leading to a significant increase in shelf life. This study aimed to address the oxidative changes taking place in pork loins during prolonged storage at SC temperature. Loins were stored either at chilling (CH) conditions (2-4 °C) for 4 weeks or at SC temperature (around -1 °C) for 12 weeks. Storage at SC temperature diminished the rate of lipid and protein oxidation and discoloration in pork loins, so that final levels of most oxidation products and instrumental color values after 12 weeks of SC storage were similar to those after 4 weeks at CH conditions. However, hexanal content peaked by the end of SC storage, pointing to a potential accumulation of compounds from lipid oxidation during SC storage. SC storage of pork slows down the rate of lipid and protein oxidation. However, accumulation of volatile compounds from lipid oxidation could be a limiting factor for shelf life. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

  10. Effect of temperature on storage modulus and glass transition temperature of ZnS/PS nanocomposites

    NASA Astrophysics Data System (ADS)

    Agarwal, Sonalika; Awasthi, Kamlendra; Saxena, N. S.

    2018-05-01

    In the present study, a simplified solution casting method has been used for preparation of ZnS/PS nanocomposites, based on mixing the ZnS nano filler in nanometer range with the polymer matrix. The prepared nanocomposites with different concentration (0, 2, 4, 6 & 8 wt %) are structurally characterized through X-ray diffraction (XRD) and transmission electron microscope (TEM). The main objective of this study is to investigate the variation of storage modulus and glass transition temperature (Tg) within temperature range 30oC to 150oC for PS and ZnS/PS nanocomposites and have been performed through dynamic mechanical analyzer (DMA). The result shows that storage modulus and Tg of nanocomposites increase with the increase of ZnS nanoparticles up to 4 wt. % in PS and beyond this wt. %, both storage modulus and Tg decrease. The increasing behavior is due to the good adhesion between the ZnS nanoparticles and PS matrix which indicates that ZnS nanoparticles are capable of reinforcing the PS matrix. Beside this the decreasing behaviour at higher filler concentration (6 and 8 wt. %) is due to the agglomeratation of nanoparticles in polymer matrix.

  11. High Density Methane Storage in Nanoporous Carbon

    NASA Astrophysics Data System (ADS)

    Rash, Tyler; Dohnke, Elmar; Soo, Yuchoong; Maland, Brett; Doynov, Plamen; Lin, Yuyi; Pfeifer, Peter; Mriglobal Collaboration; All-Craft Team

    2014-03-01

    Development of low-pressure, high-capacity adsorbent based storage technology for natural gas (NG) as fuel for advanced transportation (flat-panel tank for NG vehicles) is necessary in order to address the temperature, pressure, weight, and volume constraints present in conventional storage methods (CNG & LNG.) Subcritical nitrogen adsorption experiments show that our nanoporous carbon hosts extended narrow channels which generate a high surface area and strong Van der Waals forces capable of increasing the density of NG into a high-density fluid. This improvement in storage density over compressed natural gas without an adsorbent occurs at ambient temperature and pressures ranging from 0-260 bar (3600 psi.) The temperature, pressure, and storage capacity of a 40 L flat-panel adsorbed NG tank filled with 20 kg of nanoporous carbon will be featured.

  12. Response to Short-Term Cold Storage for Eggs of Agasicles hygrophila (Coleoptera: Chrysomelidae), a Biological Control Agent of Alligator Weed Alternanthera philoxeroides (Caryophyllales: Amaranthaceae).

    PubMed

    Liu, Yan-Hong; Jia, Dong; Yuan, Xiao-Fang; Wang, Yuan-Xin; Chi, Hsin; Ridsdill-Smith, Thomas James; Ma, Rui-Yan

    2018-05-08

    The alligator weed flea beetle, Agasicles hygrophila Selman & Vogt (Coleoptera: Chrysomelidae) has been used very successfully for the biological control of the widely-distributed invasive weed Alternanthera philoxeroides (Mart.) Griseb (Caryophyllales: Amaranthaceae). In order to extend the 'shelf life' of natural enemies released in biological control programs, cold storage has proven to be a valuable commercial procedure. To determine a suitable low temperature for storage of A. hygrophila, we conducted short-term cold storage treatments of eggs (4°C for 0.5, 1, 2, 5 d, and 7.5, 10, 15°C for 5 d and a control of 25°C; all eggs were returned to 25°C after the treatments). We evaluated the effects of these treatments on the subsequent fitness of the populations based on a demographic analysis using group-reared age-stage two-sex life tables. For 5 d storage, temperatures below 10°C had lethal effects, which were also observed at 4°C for 2 d storage. Storage at 4°C for 0.5 d did not affect the fitness of A. hygrophila, but it did not prolong the developmental time. Storage at 10°C for 5 d significantly decreased rates of population increase compared with 25°C. A. hygrophila stored at 15°C for 5 d had similar age-(stage) specific survival rates, rates of population increase, increased longevity and reproductive capability to the controls at 25°C. It is concluded that there were no significant fitness costs after 5 d storage at 15°C, which is therefore potentially a suitable storage temperature for A. hygrophila eggs.

  13. Storage temperature affects distribution of carbon, VFA, ammonia, phosphorus, copper and zinc in raw pig slurry and its separated liquid fraction.

    PubMed

    Popovic, Olga; Jensen, Lars Stoumann

    2012-08-01

    Chemical-mechanical separation of pig slurry into a solid fraction rich in dry matter, P, Cu and Zn and a liquid fraction rich in inorganic N but poor in dry matter may allow farmers to manage surplus slurry by exporting the solid fraction to regions with no nutrient surplus. Pig slurry can be applied to arable land only in certain periods during the year, so it is commonly stored prior to field application. This study investigated the effect of storage duration and temperature on chemical characteristics and P, Cu and Zn distribution between particle size classes of raw slurry and its liquid separation fraction. Dry matter, VFA, total N and ammonium content of both slurry products decreased during storage and were affected by temperature, showing higher losses at higher storage temperatures. In both products, total P, Cu and Zn concentrations were not significantly affected by storage duration or temperature. Particle size distribution was affected by slurry separation, storage duration and temperature. In raw slurry, particles larger than 1 mm decreased, whereas particles 250 μm-1 mm increased. The liquid fraction produced was free of particles >500 μm, with the highest proportions of P, Cu and Zn in the smallest particle size class (<25 μm). The proportion of particles <25 μm increased when the liquid fraction was stored at 5 °C, but decreased at 25 °C. Regardless of temperature, distribution of P, Cu and Zn over particle size classes followed a similar pattern to dry matter. Copyright © 2012 Elsevier Ltd. All rights reserved.

  14. Influence of temperature fluctuations during cryopreservation on vital parameters, differentiation potential, and transgene expression of placental multipotent stromal cells.

    PubMed

    Pogozhykh, Denys; Pogozhykh, Olena; Prokopyuk, Volodymyr; Kuleshova, Larisa; Goltsev, Anatoliy; Blasczyk, Rainer; Mueller, Thomas

    2017-03-11

    Successful implementation of rapidly advancing regenerative medicine approaches has led to high demand for readily available cellular suspensions. In particular, multipotent stromal cells (MSCs) of placental origin have shown therapeutic efficiency in the treatment of numerous pathologies of varied etiology. Up to now, cryopreservation is the only effective way to preserve the viability and unique properties of such cells in the long term. However, practical biobanking is often associated with repeated temperature fluctuations or interruption of a cold chain due to various technical, transportation, and stocking events. While biochemical processes are expected to be suspended during cryopreservation, such temperature fluctuations may lead to accumulation of stress as well as to periodic release of water fractions in the samples, possibly leading to damage during long-term storage. In this study, we performed a comprehensive analysis of changes in cell survival, vital parameters, and differentiation potential, as well as transgene expression of placental MSCs after temperature fluctuations within the liquid nitrogen steam storage, mimicking long-term preservation in practical biobanking, transportation, and temporal storage. It was shown that viability and metabolic parameters of placental MSCs did not significantly differ after temperature fluctuations in the range from -196 °C to -100 °C in less than 20 cycles in comparison to constant temperature storage. However, increasing the temperature range to -80 °C as well as increasing the number of cycles leads to significant lowering of these parameters after thawing. The number of apoptotic changes increases depending on the number of cycles of temperature fluctuations. Besides, adhesive properties of the cells after thawing are significantly compromised in the samples subjected to temperature fluctuations during storage. Differentiation potential of placental MSCs was not compromised after cryopreservation with constant end temperatures or with temperature fluctuations. However, regulation of various genes after cryopreservation procedures significantly varies. Interestingly, transgene expression was not compromised in any of the studied samples. Alterations in structural and functional parameters of placental MSCs after long-term preservation should be considered in practical biobanking due to potential temperature fluctuations in samples. At the same time, differentiation potential and transgene expression are not compromised during studied storage conditions, while variation in gene regulation is observed.

  15. Physiochemical and antioxidant properties of roselle-mango juice blends; effects of packaging material, storage temperature and time

    PubMed Central

    Mgaya-Kilima, Beatrice; Remberg, Siv Fagertun; Chove, Bernard Elias; Wicklund, Trude

    2015-01-01

    A study was conducted to determine the effects of packaging materials, seasonality, storage temperature and time on physiochemical and antioxidant properties of roselle-mango juice blends. Roselle extract (20%, 40%, 60%, and 80%) was mixed with mango juice and stored in glass and plastic bottles at 4°C and 28°C. Total soluble solids, pH, titratable acidity, reducing sugar, color, vitamin C, total monomeric anthocyanins, total phenols, and antioxidant activity (FRAP) were evaluated in freshly prepared juice, and after, 2, 4, and 6 months of storage. The results showed that total soluble solids, reducing sugars, and pH increased with storage times under different storage time, irrespective of packaging materials. The acidity, color, total monomeric anthocyanin, vitamin C, total phenols, and antioxidant activity decreased during storage irrespective of storage temperature and packaging material. Loss of anthocyanins, total phenols, and vitamin C content were higher in blends stored at 28°C than 4°C. PMID:25838888

  16. Changes in the quality of superchilled rabbit meat stored at different temperatures.

    PubMed

    Lan, Yang; Shang, Yongbiao; Song, Ying; Dong, Quan

    2016-07-01

    This work studied the effects of a superchilling process at two different temperatures on the shelf life and selected quality parameters of rabbit meat. As the storage time increased, the rates at which the total aerobic count, total volatile basic nitrogen, thiobarbituric acid-reactive substances and pH value increased were significantly lower in superchilled rabbit meat stored at -4°C compared to those in rabbit meat stored at -2.5°C and 4°C. SDS-PAGE analysis indicated that the decrease in storage temperature could significantly reduce the degree of protein degradation. The lightness, redness, shear force, the integrity of muscle microstructure and water holding capacity decreased with increasing storage time. Compared with the samples frozen at -18°C, superchilled rabbit meat shows a marked reduction in microstructure deterioration. These results suggest that shelf life of good-quality rabbit meat was 20d under superchilling at -2.5°C and at least 36d under superchilling at -4°C, compared with less than 6d under traditional chilled storage. Copyright © 2016 Elsevier Ltd. All rights reserved.

  17. The effects of storage on the net calorific value of wood pellets

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Lee, Jun Sian; Sokhansanj, S.; Lau, A. K.

    The wood pellet export from Canada to Europe has been increasing steadily in recent years (roughly 1.8 million ton in 2013). Due to distances involved, wood pellets remain in transit and storage for months before their final consumption. The net calorific value determines the price of wood pellet purchase in Europe. There have been concerns about the changes of net calorific values over time. In this study, the effects of storage time, storage configuration, storage temperature, and wood pellet quality on the net calorific value of wood pellets for a period of 6 months were investigated. Storage configurations were openmore » or closed and storage temperatures were 25 °C, 35 °C and 45 °C. Two types of wood pellets used were whitewood and mixed. The results in closed storage indicated that storage time had a positive effect on the net calorific value where the net calorific value increased by 1% to 2% over the storage period. In open storage, the moisture content had the most significant impact on the net calorific value. The net calorific values of the two types of wood pellets were found to be significantly different at p < 0.001. A multivariable linear regression and analyses of variance performed verified the graphical results. Lastly, the authors postulated that the higher energy potential compounds, such as aldehyde and ketone, produced during pellet storage, caused the increase in net calorific values.« less

  18. The effects of storage on the net calorific value of wood pellets

    DOE PAGES

    Lee, Jun Sian; Sokhansanj, S.; Lau, A. K.; ...

    2015-06-30

    The wood pellet export from Canada to Europe has been increasing steadily in recent years (roughly 1.8 million ton in 2013). Due to distances involved, wood pellets remain in transit and storage for months before their final consumption. The net calorific value determines the price of wood pellet purchase in Europe. There have been concerns about the changes of net calorific values over time. In this study, the effects of storage time, storage configuration, storage temperature, and wood pellet quality on the net calorific value of wood pellets for a period of 6 months were investigated. Storage configurations were openmore » or closed and storage temperatures were 25 °C, 35 °C and 45 °C. Two types of wood pellets used were whitewood and mixed. The results in closed storage indicated that storage time had a positive effect on the net calorific value where the net calorific value increased by 1% to 2% over the storage period. In open storage, the moisture content had the most significant impact on the net calorific value. The net calorific values of the two types of wood pellets were found to be significantly different at p < 0.001. A multivariable linear regression and analyses of variance performed verified the graphical results. Lastly, the authors postulated that the higher energy potential compounds, such as aldehyde and ketone, produced during pellet storage, caused the increase in net calorific values.« less

  19. Thermal storage/discharge performances of Cu-Si alloy for solar thermochemical process

    NASA Astrophysics Data System (ADS)

    Gokon, Nobuyuki; Yamaguchi, Tomoya; Cho, Hyun-seok; Bellan, Selvan; Hatamachi, Tsuyoshi; Kodama, Tatsuya

    2017-06-01

    The present authors (Niigata University, Japan) have developed a tubular reactor system using novel "double-walled" reactor/receiver tubes with carbonate molten-salt thermal storage as a phase change material (PCM) for solar reforming of natural gas and with Al-Si alloy thermal storage as a PCM for solar air receiver to produce high-temperature air. For both of the cases, the high heat capacity and large latent heat (heat of solidification) of the PCM phase circumvents the rapid temperature change of the reactor/receiver tubes at high temperatures under variable and uncontinuous characteristics of solar radiation. In this study, we examined cyclic properties of thermal storage/discharge for Cu-Si alloy in air stream in order to evaluate a potentiality of Cu-Si alloy as a PCM thermal storage material. Temperature-increasing performances of Cu-Si alloy are measured during thermal storage (or heat-charge) mode and during cooling (or heat-discharge) mode. A oxidation state of the Cu-Si alloy after the cyclic reaction was evaluated by using electron probe micro analyzer (EPMA).

  20. Degradation kinetics of the antioxidant additive ascorbic acid in packed table olives during storage at different temperatures.

    PubMed

    Montaño, A; Casado, F J; Rejano, L; Sanchez, A H; de Castro, A

    2006-03-22

    The kinetics of ascorbic acid (AA) loss during storage of packed table olives with two different levels of added AA was investigated. Three selected storage temperatures were assayed: 10 degrees C, ambient (20-24 degrees C), and 40 degrees C. The study was carried out in both pasteurized and unpasteurized product. The effect of pasteurization treatment alone on added AA was not significant. In the pasteurized product, in general AA degraded following a first-order kinetics. The activation energy calculated by using the Arrhenius model averaged 9 kcal/mol. For each storage temperature, the increase in initial AA concentration significantly decreased the AA degradation rate. In the unpasteurized product, AA was not detected after 20 days in samples stored at room temperature and AA degradation followed zero-order kinetics at 10 degrees C, whereas at 40 degrees C a second-order reaction showed the best fit. In both pasteurized and unpasteurized product, the low level of initial dehydroascorbic acid disappeared during storage. Furfural appeared to be formed during storage, mainly at 40 degrees C, following zero-order kinetics.

  1. Studies on Freezing of Shell-Fish-I

    NASA Astrophysics Data System (ADS)

    Song, Dae Jin; Konagaya, Shiro; Tanaka, Takeo

    Ark shell, Anadara broughtonii(Shrenk), are commonly eaten raw or under-done in Korea, Japan, and East Asian countries. Along with a recent remarkable development of culture fisheries, Ark shell has become one of the commercially important shell-fish species. Transportation and storage of large quantities of shell-fish is becoming increasingly important. This work was begun with this background to make clear the effects of temperature and length of storage time on the quality of frozen stored ark shell. Results are as follows : (1) There was little chang in amounts of free and expressible drip from ark shell flesh frozen stored at -40°CdegC for 6 months. Water holding capacity of the same meat was almost constant over 6 months storage. However, a mounts of both drip increased markedly after 2 months storage at -10°C. (2) Protein extractibility of ark shell flesh tended to decrease gradually from the begining when stored at -10°C, while at -20°C, the protein extractibility was stable for 3 months before decreasing gradually. However at -40°C, the protein extractibility was stable for 6 months. It was found that paramyosin was very stable even when the ark shell was frozen stored at -10°C. (3) It was observed that ark shell flesh became tough when frozen. The toughness of ark shell flesh as measured by an instrument increased with frozen storage time and increased temperature. (4) In the smooth muscle, it was histologically observed that initial small ice crystals formed between muscle bundles grew larger during frozen storage. It was found that the higher the storage temperature, the bigger the ice crystals formed. Aggregation of some muscle fiber and empty spaces between muscle bundles were observd after thawed muscles frozen stored at relatively high temperature such as -10°C.

  2. Numerical simulation of seasonal heat storage in a contaminated shallow aquifer - Temperature influence on flow, transport and reaction processes

    NASA Astrophysics Data System (ADS)

    Popp, Steffi; Beyer, Christof; Dahmke, Andreas; Bauer, Sebastian

    2015-04-01

    The energy market in Germany currently faces a rapid transition from nuclear power and fossil fuels towards an increased production of energy from renewable resources like wind or solar power. In this context, seasonal heat storage in the shallow subsurface is becoming more and more important, particularly in urban regions with high population densities and thus high energy and heat demand. Besides the effects of increased or decreased groundwater and sediment temperatures on local and large-scale groundwater flow, transport, geochemistry and microbiology, an influence on subsurface contaminations, which may be present in the urban surbsurface, can be expected. Currently, concerns about negative impacts of temperature changes on groundwater quality are the main barrier for the approval of heat storage at or close to contaminated sites. The possible impacts of heat storage on subsurface contamination, however, have not been investigated in detail yet. Therefore, this work investigates the effects of a shallow seasonal heat storage on subsurface groundwater flow, transport and reaction processes in the presence of an organic contamination using numerical scenario simulations. A shallow groundwater aquifer is assumed, which consists of Pleistoscene sandy sediments typical for Northern Germany. The seasonal heat storage in these scenarios is performed through arrays of borehole heat exchangers (BHE), where different setups with 6 and 72 BHE, and temperatures during storage between 2°C and 70°C are analyzed. The developing heat plume in the aquifer interacts with a residual phase of a trichloroethene (TCE) contamination. The plume of dissolved TCE emitted from this source zone is degraded by reductive dechlorination through microbes present in the aquifer, which degrade TCE under anaerobic redox conditions to the degradation products dichloroethene, vinyl chloride and ethene. The temperature dependence of the microbial degradation activity of each degradation step is taken into account for the numerical simulations. Hence, the simulations are performed with the code OpenGeoSys, which is especially suited for simulating coupled thermal, hydraulic and geochemical processes. The scenario simulations show an increase in the source zone emission of TCE at higher temperatures, which is primarily due to the focusing of the groundwater flow in the area of higher temperatures within the source zone and to a lesser part to an increase in TCE solubility. On the other hand, a widening of the contaminant plume and enlargement of the area for TCE biodegradation is induced, which leads to an increase in biodegradation of the chlorinated hydrocarbons. In combination almost no change in the overall ratio of degraded to emitted TCE is found, which shows that the seasonal heat storage is not negatively influencing the present TCE contamination under these assumptions. The results of this work serve to support the risk assessment for the interaction between heat storage and contaminations in the shallow subsurface and show positive interactions as well as possible conflicts.

  3. Refrigeration and cryopreservation of platelets differentially affect platelet metabolism and function: a comparison with conventional platelet storage conditions.

    PubMed

    Johnson, Lacey; Tan, Shereen; Wood, Ben; Davis, April; Marks, Denese C

    2016-07-01

    Alternatives to room temperature storage of platelets (PLTs) may be beneficial to extend the limited shelf life and support transfusion logistics in rural and military areas. The aim of this study was to assess the morphologic, metabolic, and functional aspects of PLTs stored at room temperature or in refrigerated conditions or cryopreserved. A three-arm pool-and-split study was carried out using buffy coat-derived PLTs stored in 30% plasma/70% SSP+. The three matched treatment arms were room temperature stored (20-24°C), cold-stored (2-6°C), and cryopreserved (-80°C with dimethyl sulfoxide). Liquid-stored PLTs were tested over a 21-day period, while cryopreserved PLTs were examined immediately after thawing and after 6 and 24 hours of storage at room temperature. Cold-stored and cryopreserved PLTs underwent a significant shape change, although the cryopreserved PLTs appeared to recover from this during subsequent storage. Glycolytic metabolism was reduced in cold-stored PLTs, but accelerated in cryopreserved PLTs, while oxidative phosphorylation was negatively affected by both storage conditions. PLT aggregation was potentiated by cold storage and diminished by cryopreservation in comparison to room temperature-stored PLTs. Cold storage and cryopreservation resulted in faster clot formation (R-time; thromboelastography), which was associated with an increase in microparticles. Cold storage and cryopreservation of PLTs led to morphologic and metabolic changes. However, storage under these conditions appears to maintain or even enhance certain aspects of in vitro PLT function. © 2016 AABB.

  4. Effects of storage temperature on tyramine production by Enterococcus faecalis R612Z1 in water-boiled salted ducks.

    PubMed

    Liu, Fang; Du, Lihui; Wu, Haihong; Wang, Daoying; Zhu, Yongzhi; Geng, Zhiming; Zhang, Muhan; Xu, Weimin

    2014-10-01

    Tyramine production by Enterococcus faecalis R612Z1 in water-boiled salted ducks was evaluated during storage at different temperatures. The results showed that E. faecalis R612Z1 could produce tyramine in meat samples when the storage temperature was no less than 4°C. The E. faecalis R612Z1 counts of the meat samples reached 10(8) CFU/g on day 7 at 4°C and on day 4 at 10°C. However, the tyramine content of the meat samples stored at 10°C increased to 23.73 μg/g (on day 10), which was greater than the level in the samples stored at 4°C (7.56 μg/g). Reverse transcription quantitative PCR detection of the expression level of the tyrDC gene in E. faecalis R612Z1 in the meat samples revealed no significant changes at different storage temperatures. Thus, the changes in tyramine production of E. faecalis R612Z1 may be due to the different enzymatic activities at different storage temperatures.

  5. Cold storage effects on egg hatch in laboratory-reared Culicoides variipennis sonorensis (Diptera: Ceratopogonidae).

    PubMed

    Hunt, G J; Tabachnick, W J

    1995-09-01

    The effects of cold storage (5 degrees C) on the hatching rates of laboratory-reared Culicoides variipennis sonorensis eggs were examined. Mortality increased with storage time. Average maximum embryo survivorship for 4 trials was 55.0 +/- 4.2 (+/- SEM) days. Alternating daily cycles of high and then low mean hatching rates occurred and possibly were due to location differences in temperature within the temperature-controlled rearing system. During cold storage at 5 degrees C, C. v. sonorensis eggs may be kept for ca. 28 days with an anticipated hatching rate of about 50%.

  6. Resonant structure of low-energy H3+ dissociative recombination

    NASA Astrophysics Data System (ADS)

    Petrignani, Annemieke; Altevogt, Simon; Berg, Max H.; Bing, Dennis; Grieser, Manfred; Hoffmann, Jens; Jordon-Thaden, Brandon; Krantz, Claude; Mendes, Mario B.; Novotný, Oldřich; Novotny, Steffen; Orlov, Dmitry A.; Repnow, Roland; Sorg, Tobias; Stützel, Julia; Wolf, Andreas; Buhr, Henrik; Kreckel, Holger; Kokoouline, Viatcheslav; Greene, Chris H.

    2011-03-01

    High-resolution dissociative recombination rate coefficients of rotationally cool and hot H3+ in the vibrational ground state have been measured with a 22-pole trap setup and a Penning ion source, respectively, at the ion storage-ring TSR. The experimental results are compared with theoretical calculations to explore the dependence of the rate coefficient on ion temperature and to study the contributions of different symmetries to probe the rich predicted resonance spectrum. The kinetic energy release was investigated by fragment imaging to derive internal temperatures of the stored parent ions under differing experimental conditions. A systematic experimental assessment of heating effects is performed which, together with a survey of other recent storage-ring data, suggests that the present rotationally cool rate-coefficient measurement was performed at 380-130+50 K and that this is the lowest rotational temperature so far realized in storage-ring rate-coefficient measurements on H3+. This partially supports the theoretical suggestion that temperatures higher than assumed in earlier experiments are the main cause for the large gap between the experimental and the theoretical rate coefficients. For the rotationally hot rate-coefficient measurement a temperature of below 3250 K is derived. From these higher-temperature results it is found that increasing the rotational ion temperature in the calculations cannot fully close the gap between the theoretical and the experimental rate coefficients.

  7. A study on cooling characteristics of clathrate compound as low temperature latent heat storage material

    NASA Astrophysics Data System (ADS)

    Kim, Chang Oh; Kim, Jin Heung; Chung, Nak Kyu

    2007-07-01

    Materials that can store low temperature latent heat are organic/inorganic chemicals, eutectic salt system and clathrate compound. Clathrate compound is the material that host compound in hydrogen bond forms cage and guest compound is included into it and combined. Crystallization of hydrate is generated at higher temperature than that of ice from pure water. And physical properties according to temperature are stable and congruent melting phenomenon is occurred without phase separation and it has relatively high latent heat. But clathrate compound still has supercooling problem occurred in the course of phase change and supercooling should be minimized because it affects efficiency of equipment very much. Therefore, various studies on additives to restrain this or heat storage methods are needed. Supercooling is the phenomenon that low temperature thermal storage material is not crystallized and existed as liquid for some time under phase change temperature. Because phase change into solid is delayed and it is existed as liquid due to this, heat transfer from low temperature thermal storage material is lowered. Therefore it is not crystallized at original phase change temperature and crystallized after cooled as much as supercooling degree and operation time of refrigerator is increased. In this study was investigated the cooling characteristics of the clathrate compound as a low temperature latent heat storage material. And additive was added to clathrate compound and its supercooling restrain effect was studied experimentally.

  8. Methods for accurate cold-chain temperature monitoring using digital data-logger thermometers

    NASA Astrophysics Data System (ADS)

    Chojnacky, M. J.; Miller, W. M.; Strouse, G. F.

    2013-09-01

    Complete and accurate records of vaccine temperature history are vital to preserving drug potency and patient safety. However, previously published vaccine storage and handling guidelines have failed to indicate a need for continuous temperature monitoring in vaccine storage refrigerators. We evaluated the performance of seven digital data logger models as candidates for continuous temperature monitoring of refrigerated vaccines, based on the following criteria: out-of-box performance and compliance with manufacturer accuracy specifications over the range of use; measurement stability over extended, continuous use; proper setup in a vaccine storage refrigerator so that measurements reflect liquid vaccine temperatures; and practical methods for end-user validation and establishing metrological traceability. Data loggers were tested using ice melting point checks and by comparison to calibrated thermocouples to characterize performance over 0 °C to 10 °C. We also monitored logger performance in a study designed to replicate the range of vaccine storage and environmental conditions encountered at provider offices. Based on the results of this study, the Centers for Disease Control released new guidelines on proper methods for storage, handling, and temperature monitoring of vaccines for participants in its federally-funded Vaccines for Children Program. Improved temperature monitoring practices will ultimately decrease waste from damaged vaccines, improve consumer confidence, and increase effective inoculation rates.

  9. Preliminary analytical study on the feasibility of using reinforced concrete pile foundations for renewable energy storage by compressed air energy storage technology

    NASA Astrophysics Data System (ADS)

    Tulebekova, S.; Saliyev, D.; Zhang, D.; Kim, J. R.; Karabay, A.; Turlybek, A.; Kazybayeva, L.

    2017-11-01

    Compressed air energy storage technology is one of the promising methods that have high reliability, economic feasibility and low environmental impact. Current applications of the technology are mainly limited to energy storage for power plants using large scale underground caverns. This paper explores the possibility of making use of reinforced concrete pile foundations to store renewable energy generated from solar panels or windmills attached to building structures. The energy will be stored inside the pile foundation with hollow sections via compressed air. Given the relatively small volume of storage provided by the foundation, the required storage pressure is expected to be higher than that in the large-scale underground cavern. The high air pressure typically associated with large temperature increase, combined with structural loads, will make the pile foundation in a complicated loading condition, which might cause issues in the structural and geotechnical safety. This paper presents a preliminary analytical study on the performance of the pile foundation subjected to high pressure, large temperature increase and structural loads. Finite element analyses on pile foundation models, which are built from selected prototype structures, have been conducted. The analytical study identifies maximum stresses in the concrete of the pile foundation under combined pressure, temperature change and structural loads. Recommendations have been made for the use of reinforced concrete pile foundations for renewable energy storage.

  10. Photoacoustic study of the influence of the cooling temperature on the CO2 emission rate by Carica papaya L. in modified atmosphere

    NASA Astrophysics Data System (ADS)

    Schramm, D. U.; Sthel, M. S.; da Silva, M. G.; Carneiro, L. O.; Silva, H. R. F.; Martins, M. L. L.; Resende, E. D.; Vitorazi, L.; Vargas, H.

    2005-06-01

    The monitoring of trace gas emitted by papaya fruits and assessments of its mass loss can contribute to improve the conditions for their storage and transport. The C02 emission rate by the papaya fruits, monitored by a commercial infrared-based gas analyzer, was influenced by the temperature and storage time. The fruits stored at temperature of 13 °C accumulated more CO2 inside the PEBD bags than those fruits stored at 6 °C. The loss of mass of the fruits progressively increased with storage time for both temperatures until the saturation of the moisture inside the PEBD bag, been more pronounced at 13 ºC.

  11. Temperature-dependent dielectric and energy-storage properties of Pb(Zr,Sn,Ti)O3 antiferroelectric bulk ceramics

    NASA Astrophysics Data System (ADS)

    Chen, Xuefeng; Liu, Zhen; Xu, Chenhong; Cao, Fei; Wang, Genshui; Dong, Xianlin

    2016-05-01

    The dielectric and energy-storage properties of Pb0.99Nb0.02[(Zr0.60Sn0.40)0.95Ti0.05]0.98O3 (PNZST) bulk ceramics near the antiferroelectric (AFE)-ferroelectric (FE) phase boundary are investigated as a function of temperature. Three characteristic temperatures T0, TC, T2 are obtained from the dielectric temperature spectrum. At different temperature regions (below T0, between T0 and TC, and above TC), three types of hysteresis loops are observed as square double loop, slim loop and linear loop, respectively. The switching fields and recoverable energy density all first increase and then decrease with increasing temperature, and reach their peak values at ˜T0. These results provide a convenient method to optimize the working temperature of antiferroelectric electronic devices through testing the temperature dependent dielectric properties of antiferroelectric ceramics.

  12. Effect of Cold Storage on Biological Traits of Psix saccharicola (Hymenoptera: Platygastridae), an Egg Parasitoid of Acrosternum arabicum (Hemiptera: Pentatomidae).

    PubMed

    Forouzan, Farzane; Jalali, Mohammad Amin; Ziaaddini, Mahdi; Hashemi Rad, Hamid

    2018-05-28

    Psix saccharicola (Mani) (Hymenoptera: Platygastridae) is a solitary egg parasitoid of the pistachio green stink bug, Acrosternum arabicum (Wagner) (Hemiptera: Pentatomidae), which is one of the most important pests of pistachio in Iran. Augmentation of P. saccharicola field populations using mass-reared individuals may provide an alternative to conventional pesticide use for pistachio green stink bug control. Cold storage is an important component of mass-rearing protocols for optimum timing of host egg parasitization and potentially extended storage of P. saccharicola pupae prior to adult emergence. The impact of cold storage on A. arabicum eggs for various time intervals at 4.0°C was investigated. Results indicated that host eggs stored at 4.0°C for up to 60 d could be exploited by P. sacchricola, whereas no offspring were produced when eggs were stored for 120 d. The emergence rates of the F1 and F2 generations declined with increased host egg storage time. Both sex ratio and survival rate of the F2 generation decreased as the refrigeration time of host eggs increased. The impact of cold storage on P. saccharicola pupae was evaluated. Reared pupae of P. saccharicola were held for 1 wk at three temperatures and compared with a control (27 ± 1°C). Psix saccharicola pupae were tolerant to cold storage at 8 and 12°C. Cold storage adversely affected mean adult emergence at 4°C, which decreased following low temperature exposure. Furthermore, mean percentage survivorship was unaffected by storage at low temperatures in the F1 generation, but was reduced at 4°C. The sex ratio of the F1 generation became more male-biased when held at lower storage temperatures. The highest female proportion was observed at 12°C.

  13. Effect of time, temperature, and slicing on respiration rate of mushrooms.

    PubMed

    Iqbal, T; Rodrigues, F A S; Mahajan, P V; Kerry, J P

    2009-08-01

    Respiration rate measurement considering the effects of cutting, temperature, and storage time are important for the shelf life study and modified atmosphere-packaging design of fresh-cut produce. This study investigates in the respiration rate of fresh whole and sliced mushrooms at 0, 4, 8, 12, 16, and 20 degrees C under ambient atmosphere and different storage times. The O(2) consumption rate increased with temperature and ranged from 22.13 to 102.41 mL/(kg.h) and 28.87 to 143.22 mL/(kg.h) for whole and sliced mushrooms, respectively, in the temperature range tested. Similar trend was observed for CO(2) production rate. Slicing of mushrooms increased the respiration rate by 30% at 0 degrees C and 40% at 20 degrees C indicating that the mushrooms are not as sensitive to the stress caused by cutting as other fresh produce. Storage time affected both respiration rate of whole and sliced mushrooms and this effect was prominent at higher temperatures. The respiration rates increased initially for some time, then decreased and reached steady state value at 12, 16, and 20 degrees C. A 2nd-order polynomial equation was used to fit the respiration rate data as a function of time at each temperature tested.

  14. Effects of storage temperature on bacterial growth rates and community structure in fresh retail sushi.

    PubMed

    Hoel, S; Jakobsen, A N; Vadstein, O

    2017-09-01

    This study was conducted to assess the effects of different storage temperatures (4-20°C), on bacterial concentrations, growth rates and community structure in fresh retail sushi, a popular retail product with a claimed shelf life of 2-3 days. The maximum specific growth rate based on aerobic plate count (APC) at 4°C was 0·06 h -1 and displayed a sixfold increase (0·37 h -1 ) at 20°C. Refrigeration resulted in no growth of hydrogen sulphide (H 2 S)-producing bacteria, but this group had the strongest temperature response. The bacterial community structure was determined by PCR/DGGE (denaturing gradient gel electrophoresis). Multivariate analysis based on Bray-Curtis similarities demonstrated that temperature alone was not the major determinant for the bacterial community structure. The total concentration of aerobic bacteria was the variable that most successfully explained the differences between the communities. The dominating organisms, detected by sequencing of DNA bands excised from the DGGE gel, were Brochothrix thermosphacta and genera of lactic acid bacteria (LAB). The relationship between growth rates and storage temperatures clearly demonstrates that these products are sensitive to deviations from optimal storage temperature, possibly resulting in loss of quality during shelf life. Regardless of the storage temperature, the bacterial communities converged towards a similar structure and density, but the storage temperature determined how fast the community reached its carrying capacity. Little information is available on the microbial composition of ready-to-eat food that are prepared with raw fish, subjected to contamination during handling, and susceptible to microbial growth during cold storage. Moreover, the data are a good first possibility to simulate growth of APC, H 2 S-producing bacteria and LAB under different temperature scenarios that might occur during production, distribution or storage. © 2017 The Society for Applied Microbiology.

  15. Thermal performance and heat transport in aquifer thermal energy storage

    NASA Astrophysics Data System (ADS)

    Sommer, W. T.; Doornenbal, P. J.; Drijver, B. C.; van Gaans, P. F. M.; Leusbrock, I.; Grotenhuis, J. T. C.; Rijnaarts, H. H. M.

    2014-01-01

    Aquifer thermal energy storage (ATES) is used for seasonal storage of large quantities of thermal energy. Due to the increasing demand for sustainable energy, the number of ATES systems has increased rapidly, which has raised questions on the effect of ATES systems on their surroundings as well as their thermal performance. Furthermore, the increasing density of systems generates concern regarding thermal interference between the wells of one system and between neighboring systems. An assessment is made of (1) the thermal storage performance, and (2) the heat transport around the wells of an existing ATES system in the Netherlands. Reconstruction of flow rates and injection and extraction temperatures from hourly logs of operational data from 2005 to 2012 show that the average thermal recovery is 82 % for cold storage and 68 % for heat storage. Subsurface heat transport is monitored using distributed temperature sensing. Although the measurements reveal unequal distribution of flow rate over different parts of the well screen and preferential flow due to aquifer heterogeneity, sufficient well spacing has avoided thermal interference. However, oversizing of well spacing may limit the number of systems that can be realized in an area and lower the potential of ATES.

  16. Inactivation kinetics of Vibrio vulnificus in phosphate-buffered saline at different freezing and storage temperatures and times.

    PubMed

    Seminario, Diana M; Balaban, Murat O; Rodrick, Gary

    2011-03-01

    Vibrio vulnificus (Vv) is a pathogen that can be found in raw oysters. Freezing can reduce Vv and increase the shelf life of oysters. The objective of this study was to develop predictive inactivation kinetic models for pure cultures of Vv at different frozen storage temperatures and times. Vv was diluted in phosphate-buffered saline (PBS) to obtain about 10(7) CFU/mL. Samples were frozen at -10, -35, and -80 °C (different freezing rates), and stored at different temperatures. Survival of Vv was followed after freezing and storage at -10 °C (0, 3, 6, and 9 d) and at -35 and -80 °C (every week for 6 wk). For every treatment, time-temperature data was obtained using thermocouples in blank vials. Predictive models were developed using first-order, Weibull and Peleg inactivation kinetics. Different freezing temperatures did not significantly (α = 0.05) affect survival of Vv immediately after freezing. The combined effect of freezing and 1 wk frozen storage resulted in 1.5, 2.6, and 4.9 log10 reductions for samples stored at -80, -35, and -10 °C, respectively. Storage temperature was the critical parameter in survival of Vv. A modified Weibull model successfully predicted Vv survival during frozen storage: log10 Nt = log 10No - 1.22 - ([t/10{-1.163-0.0466T}][0.00025T(2) + 0.049325]). N(o) and N(t) are initial and time t (d) survival counts, T is frozen storage temperature, Celsius degree. Vibrio vulnificus can be inactivated by freezing. Models to predict survival of V. vulnificus at different freezing temperatures and times were developed. This is the first step towards the prediction of V. vulnificus related safety of frozen oysters.

  17. The sensitivity of terrestrial carbon storage to historical climate variability and atmospheric CO2 in the United States

    USGS Publications Warehouse

    Tian, H.; Melillo, J.M.; Kicklighter, D.W.; McGuire, A.D.; Helfrich, J.

    1999-01-01

    We use the Terrestrial Ecosystem Model (TEM, Version 4.1) and the land cover data set of the international geosphere-biosphere program to investigate how increasing atmospheric CO2 concentration and climate variability during 1900-1994 affect the carbon storage of terrestrial ecosystems in the conterminous USA, and how carbon storage has been affected by land-use change. The estimates of TEM indicate that over the past 95 years a combination of increasing atmospheric CO2 with historical temperature and precipitation variability causes a 4.2% (4.3 Pg C) decrease in total carbon storage of potential vegetation in the conterminous US, with vegetation carbon decreasing by 7.2% (3.2 Pg C) and soil organic carbon decreasing by 1.9% (1.1 Pg C). Several dry periods including the 1930s and 1950s are responsible for the loss of carbon storage. Our factorial experiments indicate that precipitation variability alone decreases total carbon storage by 9.5%. Temperature variability alone does not significantly affect carbon storage. The effect of CO2 fertilization alone increases total carbon storage by 4.4%. The effects of increasing atmospheric CO2 and climate variability are not additive. Interactions among CO2, temperature and precipitation increase total carbon storage by 1.1%. Our study also shows substantial year-to-year variations in net carbon exchange between the atmosphere and terrestrial ecosystems due to climate variability. Since the 1960s, we estimate these terrestrial ecosystems have acted primarily as a sink of atmospheric CO2 as a result of wetter weather and higher atmospheric CO2 concentrations. For the 1980s, we estimate the natural terrestrial ecosystems, excluding cropland and urban areas, of the conterminous US have accumulated 78.2 Tg C yr-1 because of the combined effect of increasing atmospheric CO2 and climate variability. For the conterminous US, we estimate that the conversion of natural ecosystems to cropland and urban areas has caused a 18.2% (17.7 Pg C) reduction in total carbon storage from that estimated for potential vegetation. The carbon sink capacity of natural terrestrial ecosystems in the conterminous US is about 69% of that estimated for potential vegetation.

  18. Highly reliable contacts for lead-salt diode lasers

    NASA Astrophysics Data System (ADS)

    Lo, W.

    1981-02-01

    In order to improve the long term reliability of lead-salt diode lasers, ohmic contacts of multilayer, thin-film structures consisting of In plus Au, Pt, Ni, and Pd have been studied. Diode lasers of PbSnTe fabricated with a variety of contacts were tested during room-temperature storage and during accelerated aging tests. The results show that contact reliability can be improved when multiple overlapping films are used. After 4500 h of baking at 60 C, lasers with In-Au-Pd-Au contacts on both sides showed the least resistance increase (10%). For lasers with In-Au-Pt-Au contacts, 1 h of baking at 60 C is equivalent to 2 d storage at room temperature. Extrapolating these results, a 70% increase in contact resistance is expected for this type of laser after 9000 d of storage at room temperature. The data also suggests that a smaller increase in contact resistance can be expected for lasers fabricated with In-Au-Ni and In-Au-Pd-Au contacts.

  19. Growth of Escherichia coli O157:H7 and Listeria monocytogenes in packaged fresh-cut romaine mix at fluctuating temperatures during commercial transport, retail storage, and display.

    PubMed

    Zeng, Wenting; Vorst, Keith; Brown, Wyatt; Marks, Bradley P; Jeong, Sanghyup; Pérez-Rodríguez, Fernando; Ryser, Elliot T

    2014-02-01

    Temperature abuse during commercial transport and retail sale of leafy greens negatively impacts both microbial safety and product quality. Consequently, the effect of fluctuating temperatures on Escherichia coli O157:H7 and Listeria monocytogenes growth in commercially-bagged salad greens was assessed during transport, retail storage, and display. Over a 16-month period, a series of time-temperature profiles for bagged salads were obtained from five transportation routes covering four geographic regions (432 profiles), as well as during retail storage (4,867 profiles) and display (3,799 profiles). Five different time-temperature profiles collected during 2 to 3 days of transport, 1 and 3 days of retail storage, and 3 days of retail display were then duplicated in a programmable incubator to assess E. coli O157:H7 and L. monocytogenes growth in commercial bags of romaine lettuce mix. Microbial growth predictions using the Koseki-Isobe and McKellar-Delaquis models were validated by comparing the root mean square error (RMSE), bias, and the acceptable prediction zone between the laboratory growth data and model predictions. Monte Carlo simulations were performed to calculate the probability distribution of microbial growth from 8,122,127,472 scenarios during transport, cold room storage, and retail display. Using inoculated bags of retail salad, E. coli O157:H7 and L. monocytogenes populations increased a maximum of 3.1 and 3.0 log CFU/g at retail storage. Both models yielded acceptable RMSEs and biases within the acceptable prediction zone for E. coli O157:H7. Based on the simulation, both pathogens generally increased <2 log CFU/g during transport, storage, and display. However, retail storage duration can significantly impact pathogen growth. This large-scale U.S. study-the first using commercial time/temperature profiles to assess the microbial risk of leafy greens-should be useful in filling some of the data gaps in current risk assessments for leafy greens.

  20. Stability and changes in astaxanthin ester composition from Haematococcus pluvialis during storage

    NASA Astrophysics Data System (ADS)

    Miao, Fengping; Geng, Yahong; Lu, Dayan; Zuo, Jincheng; Li, Yeguang

    2013-11-01

    In this paper, we investigated the effects of temperature, oxygen, antioxidants, and corn germ oil on the stability of astaxanthin from Haematococcus pluvialis under different storage conditions, and changes in the composition of astaxanthin esters during storage using high performance liquid chromatography and spectrophotometry. Oxygen and high temperatures (22-25°C) significantly reduced the stability of astaxanthin esters. Corn germ oil and antioxidants (ascorbic acid and vitamin E) failed to protect astaxanthin from oxidation, and actually significantly increased the instability of astaxanthin. A change in the relative composition of astaxanthin esters was observed after 96 weeks of long-term storage. During storage, the relative amounts of free astaxanthin and astaxanthin monoesters declined, while the relative amount of astaxanthin diesters increased. Thus, the ratio of astaxanthin diester to monoester increased, and this ratio could be used to indicate if astaxanthin esters have been properly preserved. If the ratio is greater than 0.2, it suggests that the decrease in astaxanthin content could be higher than 20%. Our results show that storing algal powder from H. pluvialis or other natural astaxanthin products under vacuum and in the dark below 4°C is the most economical and applicable storage method for the large-scale production of astaxanthin from H. pluvialis. This storage method can produce an astaxanthin preservation rate of at least 80% after 96 weeks of storage.

  1. Pre-analytical stability of the plasma proteomes based on the storage temperature

    PubMed Central

    2013-01-01

    Background This study examined the effect of storage temperature on the protein profile of human plasma. Plasma samples were stored for 13 days at -80°C, -20°C, +4°C and room temperature (20-25°C) prior to proteomic analysis. The proteomic comparisons were based on the differences of mean intensity values of protein spots between fresh plasma samples (named “time zero”) and plasma samples stored at different temperatures. To better understand the thermally induced biochemical changes that may affect plasma proteins during storage we identified proteins with different expressions with respect to the time zero sample. Results Using two-dimensional electrophoresis followed by MALDI-TOF MS and /or LC-MS/MS 20 protein spots representing 10 proteins were identified with significant differences in abundance when stored at different temperatures. Our results, in agreement with various authors, indicate that during storage for a short period (13 days) at four different temperatures plasma proteins were more affected by degradation processes at +4°C compared to the other temperatures analysed. However, we founded that numerous protein spots (vitamin D binding protein, alpha-1-antitrypsin, serotransferrin, apoplipoprotein A-I, apolipoprotein E, haptoglobin and complement factor B) decrease in abundance with increasing temperature up to 4°C, but at room temperature their intensity mean values are similar to those of time zero and -80°C. We hypothesize that these proteins are labile at 4°C, but at the same time they are stable at room temperature (20-25°C). Furthermore we have grouped the proteins based on their different sensitivity to the storage temperature. Spots of serum albumin, fibrinogen gamma chain and haptoglobin are more resistant to the higher temperatures tested, as they have undergone changes in abundance only at room temperature; conversely, other spots of serum albumin, fibrinogen beta chain and serotransferrin are more labile as they have undergone changes in abundance at all temperatures except at -80°C. Conclusions Although there are many studies concerning protein stability of clinical samples during storage these findings may help to provide a better understanding of the changes of proteins induced by storage temperature. PMID:23518135

  2. Effect of modified atmosphere packaging and temperature abuse on flavor related volatile compounds of rocket leaves (Diplotaxis tenuifolia L.).

    PubMed

    Mastrandrea, Leonarda; Amodio, Maria Luisa; Pati, Sandra; Colelli, Giancarlo

    2017-07-01

    The effect of storage conditions on flavor-related volatile composition of wild rocket ( Diplotaxis tenuifolia ) was investigated on Modified Atmosphere packed (MAP) leaves stored under isothermal and non-isothermal conditions. In a first experiment the effect of MAP was compared to the storage in air at 5 °C; a second experiment aimed to study the effect of non isothermal conditions, with two temperature abuses (at 13 °C for 24 h) during a 5 °C. Twenty-four volatiles were detected, including C6, C5, isothiocyanate, lipid-derived and sulfur compounds. In the first experiment, MAP-stored rocket showed a slower loss of typical flavour volatiles (thiocyanates and isothiocyanates) and a slower production of off-flavors until 6 days of storage, compared to leaves stored in air. After this time, dimethyl sulfide and acetaldehyde dramatically increased in MAP-stored rocket samples. In the second experiment, samples stored under non-isothermal conditions showed lower O 2 and higher CO 2 concentrations than samples stored under isothermal conditions. Rocket leaves stored under non-isothermal conditions showed an increased production of volatiles responsible of off-flavors (acetaldehyde and dimethyl sulfide) following temperature abuse comparing to storage in isothermal condition. Thus, dimethyl sulfide and acetaldehyde could be effective markers for tracking the effect of temperature fluctuations on rocket during storage.

  3. Development of a Threshold Model to Predict Germination of Populus tomentosa Seeds after Harvest and Storage under Ambient Condition

    PubMed Central

    Wang, Wei-Qing; Cheng, Hong-Yan; Song, Song-Quan

    2013-01-01

    Effects of temperature, storage time and their combination on germination of aspen (Populus tomentosa) seeds were investigated. Aspen seeds were germinated at 5 to 30°C at 5°C intervals after storage for a period of time under 28°C and 75% relative humidity. The effect of temperature on aspen seed germination could not be effectively described by the thermal time (TT) model, which underestimated the germination rate at 5°C and poorly predicted the time courses of germination at 10, 20, 25 and 30°C. A modified TT model (MTT) which assumed a two-phased linear relationship between germination rate and temperature was more accurate in predicting the germination rate and percentage and had a higher likelihood of being correct than the TT model. The maximum lifetime threshold (MLT) model accurately described the effect of storage time on seed germination across all the germination temperatures. An aging thermal time (ATT) model combining both the TT and MLT models was developed to describe the effect of both temperature and storage time on seed germination. When the ATT model was applied to germination data across all the temperatures and storage times, it produced a relatively poor fit. Adjusting the ATT model to separately fit germination data at low and high temperatures in the suboptimal range increased the models accuracy for predicting seed germination. Both the MLT and ATT models indicate that germination of aspen seeds have distinct physiological responses to temperature within a suboptimal range. PMID:23658654

  4. Good vaccination practice: it all starts with a good vaccine storage temperature.

    PubMed

    Vangroenweghe, Frédéric

    2017-01-01

    Recent introduction of strategies to reduce antibiotic use in food animal production implies an increased use of vaccines in order to prevent the economic impact of several important diseases in swine. Good Vaccination Practice (GVP) is an overall approach on the swine farm aiming to obtain maximal efficacy of vaccination through good storage, preparation and finally correct application to the target animals. In order to have a better insight into GVP on swine farms and the vaccine storage conditions, a survey on vaccination practices was performed on a farmers' fair and temperatures in the vaccine storage refrigerators were measured during farm visits over a period of 1 year. The survey revealed that knowledge on GVP, such as vaccine storage and handling, needle management and injection location could be improved. Less than 10% had a thermometer in their vaccine storage refrigerator on the moment of the visit. Temperature measurement revealed that only 71% of the measured refrigerators were in line with the recommended temperature range of +2 °C to +8 °C. Both below +2 °C and above +8 °C temperatures were registered during all seasons of the year. Compliance was lower during summer with an average temperature of 9.2 °C while only 43% of the measured temperatures were within the recommended range. The present study clearly showed the need for continuous education on GVP for swine veterinarians, swine farmers and their farm personnel in general and vaccine storage management in particular. In veterinary medicine, the correct storage of vaccines is crucial since both too low and too high temperatures can provoke damage to specific vaccine types. Adjuvanted killed or subunit vaccines can be damaged (e.g. structure of aluminiumhydroxide in adjuvans) by too low temperatures (below 0 °C), whereas lyophilized live vaccines are susceptible (e.g. loss of vaccine potency) to heat damage by temperatures above +8 °C. In conclusion, knowledge and awareness of GVP and vaccine storage conditions are crucial under practical field conditions in swine herds. Focus on a correct on-farm vaccine storage is part of the responsible veterinarians' guidance in order to obtain the required vaccine efficacy.

  5. Changes in Postfermentation Quality during the Distribution Process of Anchovy ( Engraulis japonicus) Fish Sauce.

    PubMed

    Joung, Byung Chun; Min, Jin Gi

    2018-06-01

    In the present study, we evaluated the changes in quality that can occur during the distribution of nonheated anchovy ( Engraulis japonicus) fish sauce after packaging. The pH values of all samples ranged from 5.5 to 5.8, and there were no significant differences ( P > 0.05) in pH among the samples during storage regardless of storage temperature or salt concentration. The initial total volatile base nitrogen concentration in all samples after bottling was 115 to 121 mg/100 mL, but this concentration increased gradually with storage time. After 1 year of storage, total volatile base nitrogen concentration had increased to approximately 170% of the initial concentration (166 to 194 mg/100 mL). Amino nitrogen increased slightly during storage but was significantly lower than the increase in amino nitrogen during general anchovy fish sauce fermentation with anchovy flesh. Most of the free amino acids increased slightly during the storage period regardless of storage temperature or salt concentration, but tyrosine and histidine increased and then decreased during the storage period. The histamine concentration of the anchovy fish sauce at a salt concentration of 20% was 43.3 mg/100 mL initially, but after 1 year the histamine concentration was 89.7 mg/100 mL in samples stored at 10°C, 102.6 mg/100 mL in samples stored at 25°C, and 116.8 mg/100 mL in samples stored at 35°C . Changes in putrescine and cadaverine concentrations were similar to those in histamine; concentrations increased about twofold from the initial concentrations after 1 year of storage. However, the rate of increase in putrescine from 4 months after storage was very high, and cadaverine slightly decreased by 12 months of storage. High scores for umami and aroma sensory characteristics were given to samples stored at 10°C, but samples stored 35°C were given high scores for rancid. Despite the overall low scores for aroma and umami for samples stored at 35°C, the quality of the anchovy fish sauce as a fermented food was considered acceptable.

  6. Storage of Unfed and Leftover Pasteurized Human Milk.

    PubMed

    Meng, Ting; Perrin, Maryanne T; Allen, Jonathan C; Osborne, Jason; Jones, Frances; Fogleman, April D

    2016-12-01

    To determine the impact of storage on bacterial growth and immunological activity of pasteurized human milk and leftover pasteurized human milk that has been exposed to the microflora in an infant's mouth. Eighteen mother-infant dyads participated in two separate studies. Mother's milk was pasteurized, and each baby was fed 1 to 2 ounces. Pasteurized and leftover pasteurized milk were stored at room (24°C) and refrigerated temperatures (4°C). After storage, milk was analyzed for bacteria, total protein, lysozyme activity, and secretory immunoglobulin A (SIgA) activity. In pasteurized and leftover pasteurized milk stored in the refrigerator for 7 days, total aerobic bacteria do not increase significantly and total protein and bioactive proteins are stable. At room temperature, there is a significant increase in total aerobic bacteria in leftover pasteurized milk during 12 hours of storage (p < 0.01) and a significant decrease in total protein and SIgA activity in pasteurized milk during 12 hours of storage (p = 0.02 and p = 0.03, respectively). When stored in the refrigerator, pasteurized and leftover pasteurized milk may be stored for at least 7 days when considering the variables studied. Caution should be used when storing pasteurized and leftover pasteurized milk at room temperature to prevent an increase in bacterial growth and a decrease in total protein and SIgA activity.

  7. Effects of Temperature on In Vitro Short-Term Storage of Sterlet Sturgeon (Acipenser Ruthenus) Ova.

    PubMed

    Linhart, O; Shelton, W L; Tučková, V; Rodina, M; Siddique, Mam

    2016-02-01

    Artificial propagation of sturgeons is becoming increasingly important for recovery efforts as well as for commercial production. Sterlet Acipenser ruthenus is a common Eurasian sturgeon with a small body size and one of the fastest reproductive cycles among the sturgeons. The practical question being addressed in this study was how long fertilization of ovulated eggs can be delayed without substantially reducing the hatching rate, and an ancillary question is under what' temperature conditions do eggs retain good quality. Broodstock were injected with homogenized carp pituitary extract (CPE); ovulated eggs from three females were allocated to various treatment groups for temperature storage (control, 7°C, 11°C, 15°C and 19°C) until fertilized. Storage times at the regulated temperatures prior to fertilization were for 2.5, 5.0, 7.5 and 10.0 h. After the selected storage times in ovarian fluid, eggs were fertilized and transferred to incubation cages and then they were counted. Three replicates were allocated to each storage period and temperature. Hatched larvae were counted at 7-day post-fertilization. We found that sterlet eggs do not need to be fertilized immediately after collection. Reasonably good quality was retained for several hours if temperature conditions are fairly cool and stable. Eggs retained good quality when stored at 7°C and 11°C for up to 10 h with 54.1 ± 2.9 to 69.9 ± 7.9% hatching success, but egg quality was significantly reduced after 5-h storage at 19°C (p < 0.01) and 7.5-h storage at 15°C (p < 0.05) compared to cooler temperatures. Uniform temperatures between 7°C and 11°C can be considered as appropriate for storage of eggs in ovarian fluid for up to 10 h. This information can have practical application to routine hatchery practice for acipenserids, as well as for certain research protocols. © 2015 Blackwell Verlag GmbH.

  8. Effects of processing and storage conditions of cocoyam strips on the quality of fries.

    PubMed

    Oguntowo, Oyindamola; Obadina, Adewale O; Sobukola, Olajide P; Adegunwa, Mojisola O

    2016-11-01

    The effects of blanching time and temperature on the sensory and textural properties of frozen cocoyam strips were studied for cocoyam varieties. The most preferred variety after sensory evaluation was blanched at 90°C for 5 min, reproduced, and frozen at a temperature of -18°C for storage studies over a period of 12 weeks with Irish potato as control. Sensory evaluation and instrumental texture analysis of frozen fried samples were conducted at 3 weeks intervals for 12 weeks. Sensory evaluation during storage showed no significant difference ( P  < 0.05) in taste, aroma, and mouth feel attributes between control and cocoyam fries. The sensory score for taste, sogginess, and mouth feel increased while those for aroma and color decreased in comparison with the control fries over storage. The texture increased during storage and for control fries. There was a significant negative correlation between sogginess, hardness, and dry matter, respectively.

  9. The effect of storage conditions on the hygiene and sensory status of wild boar meat.

    PubMed

    Borilova, G; Hulankova, R; Svobodova, I; Jezek, F; Hutarova, Z; Vecerek, V; Steinhauserova, I

    2016-08-01

    The aim of this study was to compare hygiene status of wild boar meat (shoulder and leg) stored up to 21days at 0°C, 7°C or 15°C. The microbial counts increased gradually in the expected sequence of increasing storage temperatures, with TVC at the end of storage ranging from approx. 2logCFU/g (0°C) to 5logCFU/g (15°C). The lactic acid bacteria and psychrotrophic microflora didn't exceed 2logCFU/g and 2.5logCFU/g, respectively. Whereas odor of the meat stored at 0°C and 7°C was still acceptable at the end of storage, the odor of the meat stored at 15°C was barely acceptable after only 7d of storage and also the content of ammonia was significantly higher. Game meat obtained from animals hunted in the correct way and stored at low temperatures had good microbiological and hygiene status which could be maintained for more than 15days of storage. Copyright © 2016 Elsevier Ltd. All rights reserved.

  10. Steady and dynamic shear rheological behavior of semi dilute Alyssum homolocarpum seed gum solutions: influence of concentration, temperature and heating-cooling rate.

    PubMed

    Alaeddini, Behzad; Koocheki, Arash; Mohammadzadeh Milani, Jafar; Razavi, Seyed Mohammad Ali; Ghanbarzadeh, Babak

    2018-05-01

    Alyssum homolocarpum seed gum (AHSG) solution exhibits high viscosity at low shear rates and has anionic features. However there is no information regarding the flow and dynamic properties of this gum in semi-dilute solutions. The present study aimed to investigate the dynamic and steady shear behavior of AHSG in the semi-dilute region. The viscosity profile demonestrated a shear thinning behavior at all temperatures and concentrations. An increase in the AHSG concentration was acompanied by an increase in the pseudoplasticity degree, whereas, by increasing the temperature, the pseudoplasticity of AHSG decreased. At low gum concentration, solutions had more viscosity dependence on temperature. The mechanical spectra obtained from the frequency sweep experiment demonstrated viscoelastic properties for gum solutions. AHSG solutions showed typical weak gel-like behavior, revealing G' greater than G' within the experimental range of frequency (Hz), with slight frequency dependency. The influence of temperature on viscoelastic properties of AHSG solutions was studied during both heating (5-85 °C) and cooling (85-5 °C) processes. The complex viscosity of AHSG was greater compared to the apparent viscosity, indicating the disruption of AHSG network structure under continuous shear rates and deviation from the Cox-Merz rule. During the initial heating, the storage modulus showed a decreasing trend and, with a further increase in temperature, the magnitude of storage modulus increased. The influence of temperature on the storage modulus was considerable when a higher heating rate was applied. AHSG can be applied as a thickening and stabilizing agents in food products that require good stability against temperature. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

  11. Direct Measurements of Water Canopy Storage Capacity of Broadleaf Shrubs under Different Temperature and Wetting Regimes

    NASA Astrophysics Data System (ADS)

    Yerk, W.

    2016-12-01

    It is generally agreed that canopy water storage capacity is one of the defining factors of rainfall interception. Multiple studies of storage capacity by shrubs have been published. However, only a fraction of species have been studied. In the presented study the storage capacity of five species (Aronia melanocarpa, Cornus sericea, Hydrangea quercifolia, Itea virginica, and Prunus laurocerasus) was directly measured in an indoor experiment. Effect of the water temperature on the amount of water stored by the canopy was also investigated. Five branches of each species (length 0.25-0.60 m, LAI 1.3-3.6) were selected. Methods of full submergence in water and a simulated rain of intensity of 187.5±9.9 mm/hr were applied. Water of two different temperatures of 30°C and 1.5°C was used for the submergence method. Weight of the branches fixated in a natural position was measured with a digital balance. Storage capacity was expressed as a depth of water retained by the entire branch divided by the one-sided area of all leaves. The storage capacity obtained by submergence was 0.45±0.5 mm for A. melanocarpa, 0.33±0.03 mm for C. sericea, 0.40±0.02 mm for H. quercifolia, 0.48±0.05 mm for I. virginica, and 0.67±0.09 mm for P. laurocerasus. Difference in the storage capacities obtained by both methods was inconsistent. Water temperature exerted a more pronounced effect on the capacity. The canopies stored 0.01 to 0.05 mm more water (p-value < 0.005 for all species except A. melanocarpa). Our findings correspond with the range of storage capacity reported for shrub species. The directly measured storage capacity exceeds the widely used in hydrological modeling value of 0.2 mm. We were able to detect an increase of capacity to store cold water; however, the increase was below the practical level.

  12. Experimental evaluation of passive cooling using phase change materials (PCM) for reducing overheating in public building

    NASA Astrophysics Data System (ADS)

    Ahmed, Abdullahi; Mateo-Garcia, Monica; McGough, Danny; Caratella, Kassim; Ure, Zafer

    2018-02-01

    Indoor Environmental Quality (IEQ) is essential for the health and productivity of building users. The risk of overheating in buildings is increasing due to increased density of occupancy of people and heat emitting equipment, increase in ambient temperature due to manifestation of climate change or changes in urban micro-climate. One of the solutions to building overheating is to inject some exposed thermal mass into the interior of the building. There are many different types of thermal storage materials which typically includes sensible heat storage materials such as concrete, bricks, rocks etc. It is very difficult to increase the thermal mass of existing buildings using these sensible heat storage materials. Alternative to these, there are latent heat storage materials called Phase Change Materials (PCM), which have high thermal storage capacity per unit volume of materials making them easy to implement within retrofit project. The use of Passive Cooling Thermal Energy Storage (TES) systems in the form of PCM PlusICE Solutions has been investigated in occupied spaces to improve indoor environmental quality. The work has been carried out using experimental set-up in existing spaces and monitored through the summer the months. The rooms have been monitored using wireless temperature and humidity sensors. There appears to be significant improvement in indoor temperature of up to 5°K in the room with the PCM compared to the monitored control spaces. The success of PCM for passive cooling is strongly dependent on the ventilation strategy employed in the spaces. The use of night time cooling to purge the stored thermal energy is essential for improved efficacy of the systems to reduce overheating in the spaces. The investigation is carried within the EU funded RESEEPEE project.

  13. Characteristics of the starch fine structure and pasting properties of waxy rice during storage.

    PubMed

    Huang, Yu-Chan; Lai, Hsi-Mei

    2014-01-01

    Two waxy rice (TNW1 and TCSW1, exhibiting high and low amylase activity, respectively), were stored at 4 and 17 °C (polished rice) and at room temperature (paddy rice) for 15 months. The fine structure of starch isolated from the aged rice and the pasting properties of starch and rice flour were studied. After storage, the percentage of short amylopectin (AP) chains increased in TNW1, and no uniform changing pattern was observed in the chain-length (CL) distribution of TCSW1. The viscosity of starch isolated from the aged rice increased as the storage temperature and duration increased. We hypothesised that this increase was due to the hydrolysis of AP by endogenous amylase and the generation of small clusters during storage, which caused the simple dissociation of AP and a high swelling degree of starch granules during gelatinisation. Factor analysis of the first two factors associated with the characteristics of viscograms and the CL of AP explained 72% of the total variation. Copyright © 2013 Elsevier Ltd. All rights reserved.

  14. The probability of growth of Listeria monocytogenes in cooked salmon and tryptic soy broth as affected by salt, smoke compound, and storage temperature.

    PubMed

    Hwang, Cheng-An

    2009-05-01

    The objectives of this study were to examine and model the probability of growth of Listeria monocytogenes in cooked salmon containing salt and smoke (phenol) compound and stored at various temperatures. A growth probability model was developed, and the model was compared to a model developed from tryptic soy broth (TSB) to assess the possibility of using TSB as a substitute for salmon. A 6-strain mixture of L. monocytogenes was inoculated into minced cooked salmon and TSB containing 0-10% NaCl and 0-34 ppm phenol to levels of 10(2-3) cfu/g, and the samples were vacuum-packed and stored at 0--25 degrees C for up to 42 days. A total 32 treatments, each with 16 samples, selected by central composite designs were tested. A logistic regression was used to model the probability of growth of L. monocytogenes as a function of concentrations of salt and phenol, and storage temperature. Resulted models showed that the probabilities of growth of L. monocytogenes in both salmon and TSB decreased when the salt and/or phenol concentrations increased, and at lower storage temperatures. In general, the growth probabilities of L. monocytogenes were affected more profoundly by salt and storage temperature than by phenol. The growth probabilities of L. monocytogenes estimated by the TSB model were higher than those by the salmon model at the same salt/phenol concentrations and storage temperatures. The growth probabilities predicted by the salmon and TSB models were comparable at higher storage temperatures, indicating the potential use of TSB as a model system to substitute salmon in studying the growth behavior of L. monocytogenes may only be suitable when the temperatures of interest are in higher storage temperatures (e.g., >12 degrees C). The model for salmon demonstrated the effects of salt, phenol, and storage temperature and their interactions on the growth probabilities of L. monocytogenes, and may be used to determine the growth probability of L. monocytogenes in smoked seafood.

  15. Making the case for high temperature low sag (htls) overhead transmission line conductors

    NASA Astrophysics Data System (ADS)

    Banerjee, Koustubh

    The future grid will face challenges to meet an increased power demand by the consumers. Various solutions were studied to address this issue. One alternative to realize increased power flow in the grid is to use High Temperature Low Sag (HTLS) since it fulfills essential criteria of less sag and good material performance with temperature. HTLS conductors like Aluminum Conductor Composite Reinforced (ACCR) and Aluminum Conductor Carbon Composite (ACCC) are expected to face high operating temperatures of 150-200 degree Celsius in order to achieve the desired increased power flow. Therefore, it is imperative to characterize the material performance of these conductors with temperature. The work presented in this thesis addresses the characterization of carbon composite core based and metal matrix core based HTLS conductors. The thesis focuses on the study of variation of tensile strength of the carbon composite core with temperature and the level of temperature rise of the HTLS conductors due to fault currents cleared by backup protection. In this thesis, Dynamic Mechanical Analysis (DMA) was used to quantify the loss in storage modulus of carbon composite cores with temperature. It has been previously shown in literature that storage modulus is correlated to the tensile strength of the composite. Current temperature relationships of HTLS conductors were determined using the IEEE 738-2006 standard. Temperature rise of these conductors due to fault currents were also simulated. All simulations were performed using Microsoft Visual C++ suite. Tensile testing of metal matrix core was also performed. Results of DMA on carbon composite cores show that the storage modulus, hence tensile strength, decreases rapidly in the temperature range of intended use. DMA on composite cores subjected to heat treatment were conducted to investigate any changes in the variation of storage modulus curves. The experiments also indicates that carbon composites cores subjected to temperatures at or above 250 degree Celsius can cause permanent loss of mechanical properties including tensile strength. The fault current temperature analysis of carbon composite based conductors reveal that fault currents eventually cleared by backup protection in the event of primary protection failure can cause damage to fiber matrix interface.

  16. Combined effect of storage temperature and water activity on the antiglycoxidative properties and color of dehydrated apples.

    PubMed

    Lavelli, Vera

    2009-12-23

    Phytochemical contents, color, and inhibition efficacy toward oxidative and glycoxidative reactions were studied in dehydrated apples following storage in the water activity range from 0.1 to 0.7 at 20, 30, and 40 degrees C, which can be considered as room conditions. Hunter colorimetric parameters were analyzed at different temperatures and time intervals, and nonenzymatic browning was modeled according to pseudo-zero-order kinetics. The effect of temperature on the browning rate followed the Arrhenius equation, with an activation energy of 64000 J/mol, which was not affected by the water activity level. The phytochemical contents, inhibition efficacy of protein glycation, and antioxidant properties were then analyzed in the products stored under selected "equivalent" conditions in terms of browning effects, namely, 120 days/20 degrees C, 50 days/30 degrees C, and 22 days/40 degrees C. After storage for 120 days/20 degrees C, the retention percentages of hydroxycinnamic acids, phloridzin, and epicatechin were >86%, but ascorbic acid, catechin, and procyanidins were less stable; concurrently dehydrated apples retained about 80% of the radical scavenging activity and 70% of the ability to inhibit protein glycation. Following storage at higher temperatures the expected browning effect occurred in a shorter time scale; however, the patterns of product degradation were different. A sharp increase in the degradation rates of all antioxidants, relative to browning rate, was observed at temperatures >or=30 degrees C, and this trend was accelerated with concurrent increase in water activity at >0.3 levels. The application of low-temperature/long-time conditions for storage of dehydrated apples corresponded to maximum retention of their efficacy to counteract oxidative and glycoxidative reactions, which have been linked to human chronic diseases.

  17. Influence of Temperature and Humidity on the Stability of Carotenoids in Biofortified Maize (Zea mays L.) Genotypes during Controlled Postharvest Storage.

    PubMed

    Ortiz, Darwin; Rocheford, Torbert; Ferruzzi, Mario G

    2016-04-06

    Maize is a staple crop that has been the subject of biofortification efforts to increase the natural content of provitamin A carotenoids. Although significant progress toward increasing provitamin A carotenoid content in maize varieties has been made, postharvest handling factors that influence carotenoid stability during storage have not been fully established. The objectives of this study were to determine carotenoid profiles of six selected provitamin A biofortified maize genotypes at various developmental stages and assess the stability of carotenoids in maize kernels during controlled storage conditions (12 month period), including elevated temperature and relative humidity. There were no significant changes in the content of individual carotenoids within genotypes during kernel development from 45 days after pollination through the time of harvest. Carotenoid losses through traditional grain drying were also minimal (<9%). However, the stability of carotenoids in maize kernels over storage time after harvest was found to be dependent on both temperature and humidity, with variation observed among genotypes. Different forms of provitamin A carotenoids follow similar degradation rates. The genotype C17xDE3 had a degradation rate 2 times faster than those of the other genotypes evaluated (P < 0.001). These differences in carotenoid stability under controlled storage were attributed, in part, to observed differences in the physical properties of the kernels (surface area and porosity). These results support the notion that effective control of moisture content and temperature of the kernels during storage conditions is essential to reduce the speed of degradative reactions.

  18. Trapping Mode Dipolar DC Collisional Activation in the RF-Only Ion Guide of a Linear Ion Trap/Time-of-Flight Instrument for Gaseous Bio-Ion Declustering

    PubMed Central

    Webb, Ian K.; Gao, Yang; Londry, Frank A.; McLuckey, Scott A.

    2013-01-01

    The application of dipolar DC (DDC) to the RF-only ion guide (Q0) of a hybrid quadrupole/time-of-flight (QqTOF) mass spectrometer for collision-induced declustering of large bio-ions is described. As a broadband technique, ion trap DDC collision activation (CA) is employed to decluster ions simultaneously over a relatively broad mass-to-charge range. Declustering DDC CA can yield significantly narrower peaks relative to those observed in the absence of declustering methods, depending upon the extent of non-covalent adduction associated with the ions, and can also be used in conjunction with other methods, such as nozzle-skimmer collisional activation. The key experimental variables in the DDC experiment are the dipolar DC voltage (VDDC), VRF, and the time over which VDDC is applied. The VDDC/VRF ratio is key to the extent to which ion temperatures are elevated and also influences the upper mass-to-charge limit for ion storage. The VDDC/VRF ratio affects ion temperatures and upper m/z limit in opposing directions. That is, as the ratio increases, ion temperature increases whereas the upper m/z storage limit decreases. However, for a given VDDC/VRF ratio, the upper m/z storage limit can be increased by increasing VRF, at the expense of the lower m/z limit for ion storage. The key value of the approach is that it affords a relatively precise degree of control over ion temperatures as well as the time over which they are elevated to the higher temperature. The utility of the method is illustrated by the application of ion trap DDC CA in Q0 to oligonucleotide, protein, and multimeric protein complex analyte ions. PMID:24078247

  19. Changes in energy metabolism accompanying pitting in blueberries stored at low temperature.

    PubMed

    Zhou, Qian; Zhang, Chunlei; Cheng, Shunchang; Wei, Baodong; Liu, Xiuying; Ji, Shujuan

    2014-12-01

    Low-temperature storage and transport of blueberries is widely practiced in commercial blueberry production. In this research, the storage life of blueberries was extended at low temperature, but fruit stored for 30 d at 0°C pitted after 2d at room-temperature. Fruit cellular structure and physiological parameters accompanying pitting in blueberries were changed. The objective of this research was to characterise properties of energy metabolism accompanying pitting in blueberries during storage, including adenosine phosphates and mitochondrial enzymes involved in stress responses. Physiological and metabolic disorders, changes in cell ultrastructure, energy content and ATPase enzyme activity were observed in pitting blueberries. Energy shortages and increased activity of phenylalanine ammonia lyase (PAL) and lipoxygenase (LOX) were observed in fruit kept at shelf life. The results suggested that sufficient available energy status and a stable enzymatic system in blueberries collectively contribute to improve chilling tolerance, thereby alleviating pitting and maintaining quality of blueberry fruit in long-term cold storage. Copyright © 2014 Elsevier Ltd. All rights reserved.

  20. Circulating current battery heater

    DOEpatents

    Ashtiani, Cyrus N.; Stuart, Thomas A.

    2001-01-01

    A circuit for heating energy storage devices such as batteries is provided. The circuit includes a pair of switches connected in a half-bridge configuration. Unidirectional current conduction devices are connected in parallel with each switch. A series resonant element for storing energy is connected from the energy storage device to the pair of switches. An energy storage device for intermediate storage of energy is connected in a loop with the series resonant element and one of the switches. The energy storage device which is being heated is connected in a loop with the series resonant element and the other switch. Energy from the heated energy storage device is transferred to the switched network and then recirculated back to the battery. The flow of energy through the battery causes internal power dissipation due to electrical to chemical conversion inefficiencies. The dissipated power causes the internal temperature of the battery to increase. Higher internal temperatures expand the cold temperature operating range and energy capacity utilization of the battery. As disclosed, either fixed frequency or variable frequency modulation schemes may be used to control the network.

  1. Development of an Operation Control System for Photovoltaics and Electric Storage Heaters for Houses Based on Information in Weather Forecasts

    NASA Astrophysics Data System (ADS)

    Obara, Shin'ya

    An all-electric home using an electric storage heater with safety and cleaning is expanded. However, the general electric storage heater leads to an unpleasant room temperature and energy loss by the overs and shorts of the amount of heat radiation when the climate condition changes greatly. Consequently, the operation of the electric storage heater introduced into an all-electric home, a storage type electric water heater, and photovoltaics was planned using weather forecast information distributed by a communication line. The comfortable evaluation (the difference between a room-temperature target and a room-temperature result) when the proposed system was employed based on the operation planning, purchase electric energy, and capacity of photovoltaics was investigated. As a result, comfortable heating operation was realized by using weather forecast data; furthermore, it is expected that the purchase cost of the commercial power in daytime can be reduced by introducing photovoltaics. Moreover, when the capacity of the photovoltaics was increased, the surplus power was stored in the electric storage heater, but an extremely unpleasant room temperature was not shown in the investigation ranges of this paper. By obtaining weather information from the forecast of the day from an external service using a communication line, the heating system of the all-electric home with low energy loss and comfort temperature is realizable.

  2. Structural and technological characteristics of starch isolated from sorghum as a function of drying temperature and storage time.

    PubMed

    Cruz, David Bandeira da; Silva, Wagner Schellin Vieira da; Santos, Ivonir Petrarca Dos; Zavareze, Elessandra da Rosa; Elias, Moacir Cardoso

    2015-11-20

    The quality of sorghum grains can vary according to the conditions of the drying temperature and storage time. The objective of this study was to evaluate the effects of the drying temperature and storage time of sorghum grain on the structure and technological properties of starch. The sorghum grains were dried at 45, 65, and 85°C and stored for six months. The grains were stored in an environment with a controlled temperature and humidity, and the starch from sorghum grains was isolated in initial time, after three and six months. The sorghum starches grains dried at 45 and 65°C present higher relative crystallinity than the starches of sorghum grains dried at 85°C in three months of storage. A reduction in the solubility of the starches of the sorghum grains dried at 85°C was observed when the grains were stored during six months. The breakdown and swelling power of the starches were reduced with the increase of the drying temperature. Copyright © 2015 Elsevier Ltd. All rights reserved.

  3. Evaluation of annual efficiencies of high temperature central receiver concentrated solar power plants with thermal energy storage.

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Ehrhart, Brian David; Gill, David Dennis

    The current study has examined four cases of a central receiver concentrated solar power plant with thermal energy storage using the DELSOL and SOLERGY computer codes. The current state-of-the-art base case was compared with a theoretical high temperature case which was based on the scaling of some input parameters and the estimation of other parameters based on performance targets from the Department of Energy SunShot Initiative. This comparison was done for both current and high temperature cases in two configurations: a surround field with an external cylindrical receiver and a north field with a single cavity receiver. There is amore » fairly dramatic difference between the design point and annual average performance, especially in the solar field and receiver subsystems, and also in energy losses due to the thermal energy storage being full to capacity. Additionally, there are relatively small differences (<2%) in annual average efficiencies between the Base and High Temperature cases, despite an increase in thermal to electric conversion efficiency of over 8%. This is due the increased thermal losses at higher temperature and operational losses due to subsystem start-up and shut-down. Thermal energy storage can mitigate some of these losses by utilizing larger thermal energy storage to ensure that the electric power production system does not need to stop and re-start as often, but solar energy is inherently transient. Economic and cost considerations were not considered here, but will have a significant impact on solar thermal electric power production strategy and sizing.« less

  4. Techno-economic performance evaluation of direct steam generation solar tower plants with thermal energy storage systems based on high-temperature concrete and encapsulated phase change materials

    NASA Astrophysics Data System (ADS)

    Guédez, R.; Arnaudo, M.; Topel, M.; Zanino, R.; Hassar, Z.; Laumert, B.

    2016-05-01

    Nowadays, direct steam generation concentrated solar tower plants suffer from the absence of a cost-effective thermal energy storage integration. In this study, the prefeasibility of a combined sensible and latent thermal energy storage configuration has been performed from thermodynamic and economic standpoints as a potential storage option. The main advantage of such concept with respect to only sensible or only latent choices is related to the possibility to minimize the thermal losses during system charge and discharge processes by reducing the temperature and pressure drops occurring all along the heat transfer process. Thermodynamic models, heat transfer models, plant integration and control strategies for both a pressurized tank filled with sphere-encapsulated salts and high temperature concrete storage blocks were developed within KTH in-house tool DYESOPT for power plant performance modeling. Once implemented, cross-validated and integrated the new storage model in an existing DYESOPT power plant layout, a sensitivity analysis with regards of storage, solar field and power block sizes was performed to determine the potential impact of integrating the proposed concept. Even for a storage cost figure of 50 USD/kWh, it was found that the integration of the proposed storage configuration can enhance the performance of the power plants by augmenting its availability and reducing its levelized cost of electricity. As expected, it was also found that the benefits are greater for the cases of smaller power block sizes. Specifically, for a power block of 80 MWe a reduction in levelized electricity costs of 8% was estimated together with an increase in capacity factor by 30%, whereas for a power block of 126 MWe the benefits found were a 1.5% cost reduction and 16% availability increase.

  5. A Chitosan Coating Containing Essential Oil from Origanum vulgare L. to Control Postharvest Mold Infections and Keep the Quality of Cherry Tomato Fruit

    PubMed Central

    Barreto, Tainá A.; Andrade, Sonalle C. A.; Maciel, Janeeyre F.; Arcanjo, Narciza M. O.; Madruga, Marta S.; Meireles, Bruno; Cordeiro, Ângela M. T.; Souza, Evandro L.; Magnani, Marciane

    2016-01-01

    The efficacy of an edible chitosan coating (CHI; 4 mg/mL) and Origanum vulgare L. essential oil (OVEO; 1.25 μL/mL) for maintaining the quality of cherry tomato fruit during storage at room (25°C; 12 days) and cold (12°C; 24 days) temperatures was assessed. CHI and OVEO in combination showed in vitro fungicidal effects against R. stolonifer and Aspergillus niger. CHI-OVEO coating reduced the incidence of black mold and soft rot caused by these fungi in artificially contaminated cherry tomato fruit during storage at both temperatures. CHI-OVEO coating delayed the appearance of the first visible signs of black mold and soft rot in artificially contaminated cherry tomato fruit stored at room temperature by 6 days and by more than 9 days in those stored at cold temperature. At the end of storage at room and cold temperature fruit coated with CHI-OVEO showed higher firmness (>2 N/mm) and lower weight loss (>2%) compared to uncoated tomato fruit. CHI-OVEO coating delayed the decrease of lycopene, ascorbic citric acid, glucose and fructose during the storage time assessed at room or cold temperatures. The increase of catechin, myricetin, caffeic and syringic acids was higher (1–9 mg/g) in cherry tomato fruit coated with CHI-OVEO compared to uncoated fruit during the storage at both temperatures studied. CHI-OVEO coating is a feasible treatment for maintaining the storage quality of cherry tomato fruit. PMID:27877156

  6. Effects of postharvest handling conditions on internalization and growth of Salmonella enterica in tomatoes.

    PubMed

    Zhou, Bin; Luo, Yaguang; Nou, Xiangwu; Yang, Yang; Wu, Yunpeng; Wang, Qin

    2014-03-01

    Salmonella internalization in tomatoes during postharvest handling is a major food safety concern. This study was conducted to determine the effect of immersion time, immersion depth, and temperature differential between bacterial suspension and tomato pulp on the internalization of Salmonella enterica in tomato fruits. The effect of storage temperature and duration on the survival and growth of internalized Salmonella cells was also evaluated. Overall, immersion time significantly affected the incidence and extent of S. enterica internalization (P < 0.0001), with a linear correlation between immersion time and Salmonella internalization. The depth of Salmonella internalization in tomato tissues also increased with increasing immersion time. Immersion time also significantly influenced the degree to which the temperature differential affected Salmonella internalization. With an immersion time of 2 min, the temperature differential had no significant effect on Salmonella internalization (P = 0.2536). However, with an immersion time of 15 min, a significantly larger Salmonella population became internalized in tomatoes immersed in solutions with a -30°F (-16.7°C) temperature differential. Internalized S. enterica cells persisted in the core tissues during 14 days of storage. Strain type and storage duration significantly affected (P < 0.05) both the frequency detected and the population of internalized Salmonella recovered, but storage temperatures of 55 to 70°F (12.8 to 21.1°C) did not (P > 0.05). These findings indicate the importance of preventing pathogen internalization during postharvest handling.

  7. The effect of storage conditions on the composition and functional properties of blended bulk tank milk.

    PubMed

    O'Connell, A; Kelly, A L; Tobin, J; Ruegg, P L; Gleeson, D

    2017-02-01

    The objective of this study was to investigate the effects of storage temperature and duration on the composition and functional properties of bulk tank milk when fresh milk was added to the bulk tank twice daily. The bulk tank milk temperature was set at each of 3 temperatures (2, 4, and 6°C) in each of 3 tanks on 2 occasions during two 6-wk periods. Period 1 was undertaken in August and September when all cows were in mid lactation, and period 2 was undertaken in October and November when all cows were in late lactation. Bulk tank milk stored at the 3 temperatures was sampled at 24-h intervals during storage periods of 0 to 96 h. Compositional parameters were measured for all bulk tank milk samples, including gross composition and quantification of nitrogen compounds, casein fractions, free amino acids, and Ca and P contents. The somatic cell count, heat stability, titratable acidity, and rennetability of bulk tank milk samples were also assessed. Almost all parameters differed between mid and late lactation; however, the interaction between lactation, storage temperature, and storage duration was significant for only 3 parameters: protein content and concentrations of free cysteic acid and free glutamic acid. The interaction between storage temperature and storage time was not significant for any parameter measured, and temperature had no effect on any parameter except lysine: lysine content was higher at 6°C than at 2°C. During 96 h of storage, the concentrations of some free amino acids (glutamic acid, lysine, and arginine) increased, which may indicate proteolytic activity during storage. Between 0 and 96 h, minimal deterioration was observed in functional properties (rennet coagulation time, curd firmness, and heat stability), which was most likely due to the dissociation of β-casein from the casein micelle, which can be reversed upon pasteurization. Thus, this study suggests that blended milk can be stored for up to 96 h at temperatures between 2°C and 6°C with little effect on its composition or functional properties. Copyright © 2017 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

  8. Cariogenic potential of stored human milk--an in-vitro study.

    PubMed

    Hegde, Amitha M; Vikyath, Rani

    2007-01-01

    Human milk samples collected from ten lactating mothers in the K. S. Hegde Medical Hospital, Mangalore were divided into five different parts and stored at different temperatures for varying durations. The pH, buffer capacity and growth of Streptococcus mutans were assessed in each of these samples. There was a fall in pH of human milk stored at various temperatures. The buffer capacity of human milk increased with duration of storage. There was an increase in Streptococcus colony count in stored human milk proportional to the duration of storage and it increased more rapidly in case of milk stored at higher temperatures (0 degrees C - 4 degrees C) compared to the milk stored in the freezer (-19 degrees C). Milk samples stored at room temperature for 6 hours and in the freezer at -19 degrees C for 2 weeks were found to be relatively safe.

  9. Germination and Initial Growth of Eastern Cottonwood as Influenced by Moisture Stress, Temperature, and Storage

    Treesearch

    R. E. Farmer

    1967-01-01

    Germination energy of cottonwood seed decreased gradually as moisture stress increased from 0.0 to 10.0 atm; 15.0 atm inhibited germination except at 32 and 38 C. Temperature extremes of 15 and 38 C drastically reduced germination energy, and the reductive effect of 38 C was particularly marked after storage. Only 15-atm moisture stress or 15 C greatly reduced total...

  10. Characterization and Evaluation of a Mass Efficient Heat Storage Device.

    NASA Technical Reports Server (NTRS)

    Splinter, Scott C.; Blosser, Max L.; Gifford, Andrew R.

    2007-01-01

    The heat sponge is a device for mass-efficient storage of heat. It was developed to be incorporated in the substructure of a reentry or hypersonic vehicle to reduce thermal protection system requirements. The heat sponge consists of a liquid-vapor mixture contained within a number of miniature pressure vessels that can be embedded within a variety of different types of structures. As temperature is increased, pressure in the miniature pressure vessels also increases so that heat absorbed through vaporization of the liquid is spread over a relatively large temperature range. Using water as a working fluid, the heat storage capacity of the liquid-vapor mixture is many times higher than that of typical structural materials and is well above that of common phase change materials over the temperature range of 660oR to 1160oR. Prototype heat sponges were fabricated and characterized. These heat sponges consisted of 1.0 inch diameter hollow stainless steel spheres with a wall thickness of 0.020 inches which had varying percentages of their interior volumes filled with water. An apparatus to measure the heat stored in these prototype heat sponges was designed, fabricated, and verified. The heat storage capacity calculated from measured temperature histories is compared to numerical predictions.

  11. Changes in carbohydrate content in zucchini fruit (Cucurbita pepo L.) under low temperature stress.

    PubMed

    Palma, Francisco; Carvajal, Fátima; Lluch, Carmen; Jamilena, Manuel; Garrido, Dolores

    2014-03-01

    The postharvest handling of zucchini fruit includes low-temperature storage, making cold stress unavoidable. We have investigated the changes of soluble carbohydrates under this stress and its relation with weight loss and chilling injury in zucchini fruit during postharvest storage at 4 °C and 20 °C for up to 14 days. Two varieties with different degrees of chilling tolerance were compared: Natura, the more tolerant variety, and Sinatra, the variety that suffered more severe chilling-injury symptoms and weight loss. In both varieties, total soluble carbohydrates, reducing soluble carbohydrates and polyols content was generally higher during storage at 4 °C than at 20 °C, thus these parameters are related to the physiological response of zucchini fruit to cold stress. However, the raffinose content increased in Natura and Sinatra fruits during storage at 4 °C and 20 °C, although at 20 °C the increase in raffinose was more remarkable than at 4 °C in both varieties, so that the role of raffinose could be more likely related to dehydration than to chilling susceptibility of zucchini fruit. Glucose, fructose, pinitol, and acid invertase activity registered opposite trends in both varieties against chilling, increasing in Natura and decreasing in Sinatra. The increase in acid invertase activity in Natura fruit during cold storage could contribute in part to the increase of these reducing sugars, whose metabolism could be involved in the adaptation to postharvest cold storage. Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

  12. Effects of Domestic Storage and Thawing Practices on Salmonella in Poultry-Based Meat Preparations.

    PubMed

    Roccato, Anna; Uyttendaele, Mieke; Cibin, Veronica; Barrucci, Federica; Cappa, Veronica; Zavagnin, Paola; Longo, Alessandra; Catellani, Paolo; Ricci, Antonia

    2015-12-01

    Among consumer food handling practices, time-temperature abuse has been reported as one of the most common contributory factors in salmonellosis outbreaks where the evidence is strong. The present study performed storage tests of burgers, sausages, and kebabs and investigated (i) the effect of refrigerator temperatures (4°C versus 8 or 12°C, which were the temperatures recorded in 33 and 3%, respectively, of domestic refrigerators in Italy), with or without prior temperature abuse (25°C for 2 h, simulating transport of meats from shop to home), and (ii) the impact of the thawing method (overnight in the refrigerator at 8°C versus on the kitchen countertop at 23°C) on the presence and numbers of Salmonella bacteria. Storage tests were carried out on naturally or artificially (Salmonella enterica serovar Typhimurium at ca. 10 CFU/g) contaminated products, while freezing-thawing tests were conducted only on artificially contaminated products (Salmonella Typhimurium at ca. 10, 100, and 1,000 CFU/g). The results from the artificially contaminated products showed significant (P < 0.05) growth of Salmonella Typhimurium at 12°C (i.e., from ca. 8 most probable number [MPN]/g to > 710 MPN/g) in kebabs after 7 and 10 days but more moderate growth in sausages (i.e., from ca. 14 MPN/g to a maximum of 96 MPN/g after 9 days of storage). Storage of naturally contaminated burgers or sausages (contamination at or below 1 MPN/g) at 4, 8, or 12°C and a short time of temperature abuse (2 h at 25°C) did not facilitate an increase in the presence and numbers of Salmonella bacteria. Thawing overnight in the refrigerator led to either a moderate reduction or no change of Salmonella Typhimurium numbers in burgers, sausages, and kebabs. Overall, this study showed that domestic storage and thawing practices can affect food safety and that time-temperature abuse can cause a substantial increase of Salmonella numbers in some types of poultry-based meat preparations, highlighting that efforts for the dissemination of consumer guidelines on the correct storage and handling of meats need to be continued.

  13. The effect of storage temperature and duration on the microbial quality of bulk tank milk.

    PubMed

    O'Connell, A; Ruegg, P L; Jordan, K; O'Brien, B; Gleeson, D

    2016-05-01

    The dairy industry in Ireland is currently undergoing a period of expansion and, as a result, it is anticipated that milk may be stored in bulk tanks on-farm for periods greater than 48 h. The objective of this study was to investigate the effects of storage temperature and duration on microbial quality of bulk tank milk when fresh milk is added to the bulk tank twice daily. Bulk tank milk stored at 3 temperatures was sampled at 24-h intervals during storage periods of 0 to 96 h. Bulk tank milk samples were analyzed for total bacterial count (TBC), psychrotrophic bacterial count (PBC), laboratory pasteurization count (LPC), psychrotrophic-thermoduric bacterial count (PBC-LPC), proteolytic bacterial count, lipolytic bacterial count, presumptive Bacillus cereus, sulfite-reducing Clostridia (SRC), and SCC. The bulk tank milk temperature was set at each of 3 temperatures (2°C, 4°C, and 6°C) in each of 3 tanks on 2 occasions during two 6-wk periods. Period 1 was undertaken in August and September, when all cows were in mid lactation, and period 2 was undertaken in October and November, when all cows were in late lactation. None of the bulk tank bacterial counts except the proteolytic count were affected by lactation period. The proteolytic bacterial count was greater in period 2 than in period 1. The TBC and PBC of milk stored at 6°C increased as storage duration increased. The TBC did not increase with increasing storage duration when milk was stored at 2°C or 4°C but the PBC of milk stored at 4°C increased significantly between 0 and 96 h. The numbers of proteolytic and lipolytic bacteria, LPC, or PBC-LPC in bulk tank milk were not affected by temperature or duration of storage. Presumptive B. cereus were detected in 10% of all bulk tank milk samples taken over the two 6-wk periods, with similar proportions observed in both. In bulk tank milk samples, a greater incidence of SRC was observed in period 2 (20%) compared with period 1 (3%). Milk produced on-farm with minimal bacterial contamination can be successfully stored at 2°C and 4°C for up to 96h with little effect on its microbial quality. Copyright © 2016 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

  14. Metal-halide mixtures for latent heat energy storage

    NASA Astrophysics Data System (ADS)

    Chen, K.; Manvi, R.

    Some candidates for alkali metal and alkali halide mixtures suitable for thermal energy storage at temperatures 600 C are identified. A solar thermal system application which offer advantages such as precipitation of salt crystals away from heat transfer surfaces, increased thermal conductivity of phase change materials, corrosion inhibition, and a constant monotectic temperature, independent of mixture concentrations. By using the lighters, metal rich phase as a heat transfer medium and the denser, salt rich phase as a phase change material for latent heat storage, undesirable solidification on the heat transfer surface may be prevented, is presented.

  15. Metal-halide mixtures for latent heat energy storage

    NASA Technical Reports Server (NTRS)

    Chen, K.; Manvi, R.

    1981-01-01

    Some candidates for alkali metal and alkali halide mixtures suitable for thermal energy storage at temperatures 600 C are identified. A solar thermal system application which offer advantages such as precipitation of salt crystals away from heat transfer surfaces, increased thermal conductivity of phase change materials, corrosion inhibition, and a constant monotectic temperature, independent of mixture concentrations. By using the lighters, metal rich phase as a heat transfer medium and the denser, salt rich phase as a phase change material for latent heat storage, undesirable solidification on the heat transfer surface may be prevented, is presented.

  16. Combined effect of temperature and controlled atmosphere on storage and shelf-life of 'Rocha' pear treated with 1-methylcyclopropene.

    PubMed

    Gago, Custódia M L; Miguel, Maria G; Cavaco, Ana M; Almeida, Domingos P F; Antunes, Maria D C

    2015-03-01

    The combination of temperature and atmosphere composition for storage of Pyrus communis L. 'Rocha' treated with 1-methylcyclopropene was investigated. Fruits treated with 312 nl l(-1) 1-methylcyclopropene were stored at 0 ℃ and 2.5 ℃ in air and controlled atmosphere (CA) (3.04 kPa O2+ 0.91 kPa CO2). Fruits were removed from storage after 14, 26 and 35 weeks, transferred to shelf-life at approximately 22 ℃ and assessed for ripening and quality, symptoms of superficial scald and internal browning and the accumulation of biochemical compounds related to scald after 0, 1 and 2 weeks. Superficial scald occurred only in fruits stored for 35 weeks in air at 2.5 ℃. Levels of conjugated trienols and α-farnesene increased during the first 26 weeks in storage, remaining constant thereafter. During shelf-life, conjugated trienols were higher in fruits stored in air at 2.5 ℃. Internal browning developed in shelf-life after 26 weeks at 2.5 ℃. Pears in air at 2.5 ℃ were not able to stand a 2-week shelf-life after 35 weeks of storage, while fruits stored at 0 ℃ under CA ripened slowly after the same storage period. The retention of firmness during shelf-life of 1-methylcyclopropene-treated 'Rocha' pear can be overcome by elevating the storage temperature from 0 ℃ to 2.5 ℃, but CA is a required complement to avoid excessive softening after long-term storage. The ratio carotenoid/chlorophyll increased during storage and shelf-life, as plastids senesced. CA reduced the rate of chlorophyll loss during the first 14 weeks in storage, but its effect was reduced afterwards. 'Rocha' pear treated with 1-methylcyclopropene had a similar post-harvest behaviour during long-term storage at 0 ℃ in air or at 2.5 ℃ under CA. © The Author(s) 2013 Reprints and permissions: sagepub.co.uk/journalsPermissions.nav.

  17. Toward Optimal Cryopreservation and Storage for Achievement of High Cell Recovery and Maintenance of Cell Viability and T Cell Functionality.

    PubMed

    Angel, Stephanie; von Briesen, Hagen; Oh, Young-Joo; Baller, Marko K; Zimmermann, Heiko; Germann, Anja

    2016-12-01

    Cryopreservation of biological materials such as cells, tissues, and organs is a prevailing topic of high importance. It is employed not only in many research fields but also in the clinical area. Cryopreservation is of great importance for reproductive medicine and clinical studies, as well as for the development of vaccines. Peripheral blood mononuclear cells (PBMCs) are commonly used in vaccine research where comparable and reliable results between different research institutions and laboratories are of high importance. Whereas freezing and thawing processes are well studied, controlled, and standardized, storage conditions are often disregarded. To close this gap, we investigated the influence of suboptimal storage conditions during low-temperature storage on PBMC viability, recovery, and T cell functionality. For this purpose, PBMCs were isolated and exposed with help of a robotic system in a low-temperature environment from 0 up to 350 temperature fluctuation cycles in steps of 50 cycles to simulate storage conditions in large biorepositories with sample storage, removal, and sorting functions. After the simulation, the viability, recovery, and T cell functionality were analyzed to determine the number of temperature rises, which ultimately lead to significant cell damage. All studied parameters decreased with increasing number of temperature cycles. Sometimes after as little as only 50 temperature cycles, a significant effect was observed. These results are very important for all fields in which cell cryopreservation is employed, particularly for clinical and multicenter studies wherein the comparability and reproducibility of results play a crucial role. To obtain reliable results and to maintain the quality of the cells, not only the freezing and thawing processes but also the storage conditions should be controlled and standardized, and any deviations should be documented.

  18. Long-term trend analysis of reservoir water quality and quantity at the landscape scale in two major river basins of Texas, USA.

    USGS Publications Warehouse

    Patino, Reynaldo; Asquith, William H.; VanLandeghem, Matthew M.; Dawson, D.

    2016-01-01

    Trends in water quality and quantity were assessed for 11 major reservoirs of the Brazos and Colorado river basins in the southern Great Plains (maximum period of record, 1965–2010). Water quality, major contributing-stream inflow, storage, local precipitation, and basin-wide total water withdrawals were analyzed. Inflow and storage decreased and total phosphorus increased in most reservoirs. The overall, warmest-, or coldest-monthly temperatures increased in 7 reservoirs, decreased in 1 reservoir, and did not significantly change in 3 reservoirs. The most common monotonic trend in salinity-related variables (specific conductance, chloride, sulfate) was one of no change, and when significant change occurred, it was inconsistent among reservoirs. No significant change was detected in monthly sums of local precipitation. Annual water withdrawals increased in both basins, but the increase was significant (P < 0.05) only in the Colorado River and marginally significant (P < 0.1) in the Brazos River. Salinity-related variables dominated spatial variability in water quality data due to the presence of high- and low-salinity reservoirs in both basins. These observations present a landscape in the Brazos and Colorado river basins where, in the last ∼40 years, reservoir inflow and storage generally decreased, eutrophication generally increased, and water temperature generally increased in at least 1 of 3 temperature indicators evaluated. Because local precipitation remained generally stable, observed reductions in reservoir inflow and storage during the study period may be attributable to other proximate factors, including increased water withdrawals (at least in the Colorado River basin) or decreased runoff from contributing watersheds.

  19. Long-term storage effect on chemical composition, nutritional value and quality of Greek onion landrace "Vatikiotiko".

    PubMed

    Petropoulos, S A; Ntatsi, G; Fernandes, Â; Barros, L; Barreira, J C M; Ferreira, I C F R; Antoniadis, V

    2016-06-15

    The effect of storage at two temperatures (5±1 and 25±1 °C and 60-70±5% RH for both temperatures) on marketability and quality features of dry bulbs of local landrace "Vatikiotiko", "Sivan F1", "Red Cross F1" and "Creamgold" was examined. During storage measurements for fresh and dry weight of bulbs, tunic and flesh color, bulb firmness, nutritional value and mineral composition were taken at regular intervals. Storage concluded when either bulbs lost marketable quality or sprouting occurred. "Vatikiotiko" onion can be stored for at least 7months at 25±1 °C, whereas at 5±1 °C storage could be prolonged without significant marketability and quality loss. The fact that "Vatikiotiko" landrace can be considered a "storage" onion has to be capitalized in order to increase total production and yield, since storage could cover the market needs that arise throughout the year. Copyright © 2016 Elsevier Ltd. All rights reserved.

  20. Impact of broiler egg storage on the relative expression of selected blastoderm genes associated with apoptosis, oxidative stress, and fatty acid metabolism

    USDA-ARS?s Scientific Manuscript database

    Cool temperature storage of eggs prior to incubation is a frequent practice by commercial broiler hatcheries. However, continued storage beyond 7 days leads to a progressively increase in the rate of early embryonic mortality. In this study, we examined the relative expression of 31 genes associat...

  1. Evaluating the Impact of Global Warming on Water Balance of Maize by High-precision Controlled Experiment and MLCan model

    NASA Astrophysics Data System (ADS)

    Ma, Y.; Song, X.; Kumar, P.; Wu, Y.; Woo, D.; Le, P. V.; Ma, C.

    2016-12-01

    Increased temperature affects the agricultural hydrologic cycle not only by changing precipitation levels, evapotranspiration and the magnitude and timing of run-off, but also by impacting water flows and soil water dynamics. Accurate prediction of hydrologic change under global warming requires high-precision experiment and mathematical model to determine water interaction between interfaces in the soil-plant-atmosphere continuum. In this study, the weighting lysimeter and chamber were coupled to monitor water balance component dynamics of maize under controlled ambient temperature and elevated temperature of 2°C conditions. A mechanistic multilayer canopy-soil-root system model (MLCan) was used to predict hydrologic fluxes variation under different elevated temperature scenarios after calibration with experimental results. The results showed that maize growth period reduced 8 days under increased temperature of 2°C. The mean daily evapotranspiration, soil water storage change, and drainage was 2.66 mm, -2.75 mm, and 0.22 mm under controlled temperature condition, respectively. When temperature was elevated by 2°C, the average daily ET for maize significantly increased about 6.7% (p<0.05). However, there were non-significant impacts of increased temperature on the daily soil water storage change and drainage (p>0.05). Quantification of changes in water balance components induced by temperature increase for maize is critical for optimizing irrigation water management practices and improving water use efficiency.

  2. Effects of Growth Temperature and Postharvest Cooling on Anthocyanin Profiles in Juvenile and Mature Brassica oleracea.

    PubMed

    Socquet-Juglard, Didier; Bennett, Alexandra A; Manns, David C; Mansfield, Anna Katharine; Robbins, Rebecca J; Collins, Thomas M; Griffiths, Phillip D

    2016-02-24

    The effects of growth temperatures on anthocyanin content and profile were tested on juvenile cabbage and kale plants. The effects of cold storage time were evaluated on both juvenile and mature plants. The anthocyanin content in juvenile plants ranged from 3.82 mg of cyanidin-3,5-diglucoside equivalent (Cy equiv)/g of dry matter (dm) at 25 °C to 10.00 mg of Cy equiv/g of dm at 16 °C, with up to 76% diacylated anthocyanins. Cold storage of juvenile plants decreased the total amount of anthocyanins but increased the diacylated anthocyanin content by 3-5%. In mature plants, cold storage reduced the total anthocyanin content from 22 to 12.23 mg/g after 5 weeks of storage in red cabbage, while the total anthocyanin content increased after 2 weeks of storage from 2.34 to 3.66 mg of Cy equiv/g of dm in kale without having any effect on acylation in either morphotype. The results obtained in this study will be useful for optimizing anthocyanin production.

  3. Vibrational spectroscopy for the evaluation of molecular perturbations induced in fruit lipids by cold storage

    NASA Astrophysics Data System (ADS)

    Bertoluzza, A.; Bottura, G.; Filippetti, P.; Tosi, M. R.; Vasina, M.; Pratella, G. C.; Folchi, A.; Gallerani, G.

    1994-07-01

    Vibrational spectroscopy (Raman, FT-IR-ATR) has been applied for the first time to the study of the mechanism of chilling stress and the monitoring of the best operative conditions for cold storage of fruit. In particular, this work deals with some results of the application of vibrational spectroscopy to the molecular characterization of lipidic extracts of fruits (apples and pears, pulp and peel) stored at low temperatures. The results have been obtained in a cooperative interdisciplinary research project performing experiments on fruits for one year cycles under different storage conditions of temperature (0°C, 8°C) and atmosphere (normal, controlled). The Raman spectra, useful for the evaluation of the transition temperature and the cooperative effect in the fruit membrane lipids, were masked by the strong resonance spectrum of carotenoids. The lipid unsaturation, the natural response to cold storage, was evaluated in the FT-IR-ATR spectra and expressed as the "total" unsaturation degree R = I{3012 cm -1}/{2858 cm -1}. The results on pulp and peel lipids have shown that the R value, higher in the pulps than peels, is dependent on the storage temperature and time. The increase in R is correlated with the higher fruit resistance to the chilling stress. Furthermore, the FT-IR spectra of the outer part of the fruits stored at 8°C show modifications of the carbonylic band at 1738 cm -1 (esteric group) such as the appearance of two other bands at 1715 and 1700 cm -1 increasing in intensity with storage time. These new components can be considered as molecular markers of the onset of a hydrolysis reaction and also of a partial peroxidation of the acylic unsaturated chains.

  4. Effects of storage temperature and duration on release of antimony and bisphenol A from polyethylene terephthalate drinking water bottles of China.

    PubMed

    Fan, Ying-Ying; Zheng, Jian-Lun; Ren, Jing-Hua; Luo, Jun; Cui, Xin-Yi; Ma, Lena Q

    2014-09-01

    We investigated effects of storage temperature and duration on release of antimony (Sb) and bisphenol A (BPA) from 16 brands of polyethylene terephthalate (PET) drinking water bottles in China. After 1-week storage, Sb release increased from 1.88-8.32 ng/L at 4 °C, to 2.10-18.4 ng/L at 25 °C and to 20.3-2604 ng/L at 70 °C. The corresponding releases for BPA were less at 0.26-18.7, 0.62-22.6, and 2.89-38.9 ng/L. Both Sb and BPA release increased with storage duration up to 4-week, but their releasing rates decreased with storage time, indicating that Sb and BPA release from PET bottles may become stable under long term storage. Human health risk was evaluated based on the worst case, i.e., storage at 70 °C for 4-week. Chronic daily intake (CDI) caused by BPA release was below USEPA regulation, Sb release in one brand exceeded USEPA regulated CDI (400 ng/kg bw/d) with values of 409 and 1430 ng/kg bw/d for adult and children. Copyright © 2014 Elsevier Ltd. All rights reserved.

  5. Optimizing storage temperature of liquid bovine semen diluted in INRA96.

    PubMed

    Murphy, Edel M; O' Meara, Ciara; Eivers, Bernard; Lonergan, Patrick; Fair, Sean

    2018-06-01

    Temperature regulation of liquid bovine semen can be difficult in field situations. Two experiments were carried out to assess the effect of storage temperature on in vitro sperm characteristics and 60-d nonreturn rate (NRR) following artificial insemination (AI) of liquid bovine semen. In experiment 1, the effect of storage of liquid bovine semen in INRA96 diluent (IMV Technologies, L'Aigle, France) at 1 of 5 storage temperatures (5, 15, or 28°C, and fluctuating between 5 and 15°C or 5 and 28°C) on total and progressive motility and kinematic parameters was assessed objectively via computer-assisted sperm analyzer on d 0, 1, 2, 3, and 4 after collection. Fluctuating temperatures were designed to mimic day- to nighttime variation. In experiment 2, we assessed the field fertility of liquid semen stored at a constant 5 or 15°C or in an unregulated manner and compared with that of frozen-thawed semen (total of n = 106,738 inseminations). In experiment 1, we detected a linear decrease in motility with increased duration of storage. Semen stored at a constant 15°C or fluctuating between 5 and 15°C had greater total motility than semen held at 5 or 28°C or fluctuating between 5 and 28°C; however, semen stored at 15°C and fluctuating between 5 and 15°C did not differ from each other. Semen held at a constant 5 or 15°C or fluctuating between 5 and 15°C, although not differing from each other, had higher progressive motility scores than that held at 28°C or fluctuating between 5 and 28°C. Semen stored at a constant 28°C exhibited poor motility and velocity values but had high progressive motion values compared with that all other storage temperatures; however, the other storage temperatures did not differ from each other in relation to motility kinematics. In experiment 2, semen stored at a constant 5°C resulted in a lower 60-d NRR (62.5%) than storage at constant 15°C or unregulated temperature or frozen-thawed semen (73.6, 74.6, and 74.4%, respectively. In conclusion, sperm stored in IRNA96 are quite tolerant in terms of storage temperature, retaining acceptable motility between 5 and 15°C. Storing semen at a constant 15°C resulted in greater in vitro sperm motility and higher NRR rates than storage at 5°C and did not differ in NRR from frozen-thawed semen or semen stored at an unregulated temperature; however, lower storage temperatures were shown to be more detrimental to sperm in vivo than unregulated storage conditions. Copyright © 2018 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

  6. Antioxidant compounds of kiwifruit during post-ripening process at ambient temperature

    NASA Astrophysics Data System (ADS)

    Liang, D.; Lv, X. L.; Wang, J.; Xia, H.; Xie, Y.; Li, M. Z.; Wang, Y. Z.

    2017-09-01

    Kiwifruit is well-known for an excellent source of antioxidants. In this study, contents of total phenolics (TPC), total flavonoids (TFC), total flavanols (TFAC) and vitamin C were investigated in different fruit tissues during post-ripening process at ambient temperature. The results explored that TPC and TFC showed declining trend with the increase in storage interval in different tissues. TFAC raised with the increase in storage interval in different fruit tissues, while was followed a decrease in later process. Vitamin C content was stable in outer and inner pericarp in prometaphase of post-ripening.

  7. Impact of storage environment on the efficacy of hermetic storage bags.

    PubMed

    Lane, Brett; Woloshuk, Charles

    2017-05-01

    Small hermetic bags (50 and 100 kg capacities) used by smallholder farmers in several African countries have proven to be a low-cost solution for preventing storage losses due to insects. The complexity of postharvest practices and the need for ideal drying conditions, especially in the Sub-Sahara, has led to questions about the efficacy of the hermetic bags for controlling spoilage by fungi and the potential for mycotoxin accumulation. This study compared the effects of environmental temperature and relative humidity at two locations (Indiana and Arkansas) on dry maize (14% moisture content) in woven polypropylene bags and Purdue Improved Crop Storage (PICS) hermetic bags. Temperature and relative humidity data loggers placed in the middle of each bag provided profiles of environmental influences on stored grain at the two locations. The results indicated that the PICS bags prevented moisture penetration over the three-month storage period. In contrast, maize in the woven bags increased in moisture content. For both bag types, no evidence was obtained indicating the spread of Aspergillus flavus from colonized maize to adjacent non-colonized maize. However, other storage fungi did increase during storage. The number of infected kernels did not increase in the PICS bags, but the numbers in the woven bags increased significantly. The warmer environment in Arkansas resulted in significantly higher insect populations in the woven bags than in Indiana. Insects in the PICS bags remained low at both locations. This study demonstrates that the PICS hermetic bags are effective at blocking the effects of external humidity fluctuations as well as the spread of fungi to non-infected kernels.

  8. Production of biodiesel from bioethanol and Brassica carinata oil: oxidation stability study.

    PubMed

    Bouaid, Abderrahim; Martinez, Mercedes; Aracil, Jose

    2009-04-01

    In the present work the synthesis from bioethanol and Brassica carinata, as alternative vegetable oil, using KOH as catalyst, has been developed and optimized by application of the factorial design and response surface methodology (RSM). Temperature and catalyst concentration were found to have significant influence on conversion. A second-order model was obtained to predict conversions as a function of temperature and catalyst concentration. The maximum yield of ester (98.04%) was obtained working with an initial concentration of catalyst (1.5%) and an operation temperature of (35 degrees C). Results show that the acid value, peroxide value, and viscosity, increased while the iodine value decreased with increasing storage time of the biodiesel sample. Fatty acid ethyl esters (biodiesel) from B. carinata oil were very stable because they did not demonstrate rapid increase in peroxide value, acid value, and viscosity with increasing storage time to a period of 12 months.

  9. Postharvest transfer and survival of Salmonella enterica serotype enteritidis on living lettuce.

    PubMed

    Waitt, J A; Kuhn, D D; Welbaum, G E; Ponder, M A

    2014-02-01

    The potential for postharvest transfer of Salmonella to 'living lettuce' is not well understood. In this study, the transfer of Salmonella enterica Enteritidis (6 log CFU g(-1) ) from worker hands or contaminated roots to leaves of living lettuce was quantified. Transfer rates of Salmonella from contaminated gloves to sequentially handled lettuce heads ranged from 94% to head 1, 82% to head 2 and 69% to head 3. On average, 2.9 ± 0.1 log CFU g(-1) (64%) Salmonella was transferred from inoculated roots to leaves resulting from typical postharvest handling activities for living lettuce. Salmonella persisted on leaves stored at recommended storage temperatures (4°C) and increased 0.5 log CFU g(-1) when stored at temperature abuse conditions (12°C). Salmonella increased 1.6 log CFU g(-1) on roots after 18-day storage at 12°C, emphasizing the need to maintain temperature control to reduce the risk of human illness. Hydroponically grown lettuce packaged in plastic clamshells with intact roots, marketed as 'living lettuce', is increasing in popularity due to its extended shelf life. This study demonstrates the transfer of Salmonella from contaminated worker hands and contaminated roots to leaves where it persisted at 4°C for 18 day. Temperature abuse (12°C) increased Salmonella on roots and leaves. These findings suggest that failure to maintain temperatures below 12°C can pose a risk for consumers purchasing living lettuce at markets where recommended storage temperatures are not maintained. © 2013 The Society for Applied Microbiology.

  10. The impact of storage time and temperature in polyethylen bottles on the multi isotope composition of water

    NASA Astrophysics Data System (ADS)

    Schmiedinger, Iris; Böttcher, Michael E.

    2017-04-01

    Storage of water samples in polyethylen bottles for later hydrogeochemical analysis is a common practice in laboratories world-wide. It is, however, known for a long time to geochemists that aqueous solutions lose water as a function of time due to the diffusion of water molecules (similar to CO2 or H2S) through the polymer membrane, a process that is suspected to increase with rising temperature. First observations on the impact of storage on O-18 and H-2 contents in water were reported by Spangenberg & Vennemann (RCIM 2008) and Spangenberg (RCIM 2012), but no study considered systematically the effect of temperature, sofar. In the present study we carried out long-term experiments to investigate the impact of storage of fresh water in LDPE bottles for up to 18 months at 4 different temperatures (4°, 10°, 23°, and 60°C). The loss of water was followed gravimetrically, and the stable isotope composition of the water sample was analyzed with a Picarro CRDS 2140-i system. Whereas, at the low temperatures of 4° and 10°C, no measurable loss of water was observed during a storage time up to 1.5 years, a substantial loss of water was observed at 23°C and 60°C. This change was associated with, for instance at 60°C: an increase in d18O (up to 10 ‰), d2H (up to 28 ‰), d17O (up to 5 ‰). The deuterium excess was shifted towards lower values by up to 55 ‰. The magnitude of the isotope effect mostly depends on the extend of evaporation from the bottles, allowing for an estimate of the fractionation factor by considering a closed Rayleigh-type system.

  11. Effects of storage temperature on survival and infectivity of three indigenous entomopathogenic nematodes strains (Steinernematidae and Heterorhabditidae) from Meghalaya, India.

    PubMed

    Lalramliana; Yadav, Arun K

    2016-12-01

    Three locally isolated strains of entomopathogenic nematodes (EPNs), viz. Heterorhabditis indica , Steinernema thermophilum and Steinernema glaseri , from Meghalaya, India were characterized in terms of storage temperature and survival and infectivity of their infective juveniles (IJs). The survival and infectivity of nematode IJs was studied at, 5 ± 2 and 25 ± 2 °C, for a period of 120 days, using deionized water as storage medium. The viability of nematode IJs was checked by mobility criterion at different storage periods, while the infectivity of nematode IJs was ascertained on the basis of establishment of IJs, using Galleria mellonella larva mortality tests in petridishes. The results of this study revealed that storage temperature markedly affects the survival as well as the establishment of nematode IJs of the three EPN species. At 5 °C, comparatively higher rate of IJ's survival (i.e. 74-86 %) was observed for 15 days of storage, but the same reduced drastically to 28-32 % after 30 days of storage for H. indica and S. thermophilum . On the other hand, at 25 °C, the survival of nematode IJs was observed till 120 days for all the three studied EPNs. In case of S. thermophilum and S. glaseri , higher rate of IJs survival (>75 %) was observed respectively at 15 and 30 days of observation. The study also showed that the establishment of IJs of the three EPN species declines with increase in storage periods, at both the test temperatures. In general, the nematodes stored at 25 °C showed comparatively better establishment than those stored at 5 °C. Among the three EPN studied, the establishment of S. glaseri was comparatively better than the rest of the species at both the temperatures and for different storage durations. In conclusion, our study adds further valuable information about the effect of storage temperature on survival and infectivity of three indigenous EPN species of Meghalaya, India which appears to be promising biocontrol agents of local insect pests.

  12. Gamma irradiation of peanut kernel to control mold growth and to diminish aflatoxin contamination

    NASA Astrophysics Data System (ADS)

    Y.-Y. Chiou, R.

    1996-09-01

    Peanut kernel inoculated with Aspergillus parasiticus conidia were gamma irradiated with 0, 2.5, 5.0 and 10 kGy using Co60. Levels higher than 2.5 kGy were effective in retarding the outgrowth of A. parasiticus and reducing the population of natural mold contaminants. However, complete elimination of these molds was not achieved even at the dose of 10 kGy. After 4 wk incubation of the inoculated kernels in a humidified condition, aflatoxins produced by the surviving A. parasiticus were 69.12, 2.42, 57.36 and 22.28 μ/g, corresponding to the original irradiation levels. Peroxide content of peanut oils prepared from the irradiated peanuts increased with increased irradiation dosage. After storage, at each irradiation level, peroxide content in peanuts stored at -14°C was lower than that in peanuts stored at an ambient temperature. TBA values and CDHP contents of the oil increased with increased irradiation dosage and changed slightly after storage. However, fatty acid contents of the peanut oil varied in a limited range as affected by the irradiation dosage and storage temperature. The SDS-PAGE protein pattern of peanuts revealed no noticeable variation of protein subunits resulting from irradiation and storage.

  13. A proteomic approach reveals the variation in human platelet protein composition after storage at different temperatures.

    PubMed

    Wang, Shichun; Jiang, Tianlun; Fan, Yahan; Zhao, Shuming

    2018-03-29

    Cryopreservation can slow down the metabolism and decrease the risk of bacterial contamination. But, chilled platelets (PLTs) show a reduced period in circulation due to the rapid clearance by hepatic cells or spleen macrophages after transfusion. The deleterious changes that PLTs undergo are mainly considered the result of PLT protein variation. However, the basis for proteomic variation of stored PLTs remains poorly understood. Besides count, activation markers (CD62P and Annexin V), and aggregation, we used quantitative mass spectrometry to create the first comprehensive and quantitative human PLT proteome of samples stored at different temperatures (22°C, 10°C and -80°C). We found different conditions caused different platelet storage lesion (PSL). PLT count was decreased no matter at what temperature stored. PLTs viability at low temperature dropped by 21.78% and 11.21%, respectively, as compared 10.26% at room temperature, there were no significant differences between the storage methods. Membrane expression of CD62P gradually increased in all groups especially stored at 22°C up to 40% and 10°C up to 30%. However, exposure of PS on the PLT membrane was below 1% in every group. The PLT proteome showed there were 575 and 454 potential proteins identified by general iTRAQ analysis and phosphorylation iTRAQ a nalysis, respectively, among them, 33 common differentially expressed proteins caused by storage time and 44 caused by storage temperature Especially, membrane-bound proteins (such as FERMT3, STX4, MYL9 and TAGLN2) played key roles in PLT storage lesion. The pathways "Endocytosis", "Fc gamma R-mediated phagocytosis" and "Regulation of actin cytoskeleton" were affected predominantly by storage time. And the pathways "SNARE interactions in vesicular transport" and "Vasopressin-regulated water reabsorption" were affected by cold storage in our study. Proteomic results can help us to understand PLT biochemistry and physiology and thus unravel the mechanisms of PSL in time and space for more successful PLT transfusion therapy.

  14. Corrosion Behavior of Nuclear Waste Storage Canister Materials

    NASA Astrophysics Data System (ADS)

    Grant, John

    The nature of interaction of mild steel nuclear waste storage containers with technetium ions is not fully known. Technetium is formed during nuclear processing and some of this technetium has leaked at the Hanford nuclear waste storage site in Washington State. It is often found as highly oxidized pertechnetate (TeO4-) anions at these storage sites which also happen to be highly alkaline and contain a significant amount of nitrate. Theoretically, pertechnetate anions can act as electron acceptors and interact with the mild steel containers and accelerate the oxidation (corrosion) of steel. It is of interest to identify if pertechnetate anions pose a corrosion hazard to the mild steel nuclear waste storage tanks, under the conditions of the storage sites, as that can accelerate the degradation of the tanks and lead to further contamination. In this thesis, the interaction of two relevant container materials, namely, steel alloys A285 and A537 with a technetium surrogate, rhenium was studied. Perrhenate was used as an analog for pertechnetate. As all isotopes of technetium are radioactive, rhenium was chosen as the experimental surrogate due to its chemical similarity to technetium. Electrochemical behavior was evaluated using potentiodynamic polarization tests, and the surface morphology was studied using optical microscopy and scanning electron microscopy. Potentiodynamic polarization tests were conducted in 1.0M NaNO3 + 0.1M NaOH and 1.0M NaNO3 + 0.1M NaOH + 0.02M NaReO4. Tests were performed at three different temperatures, namely, (i) room temperature, (ii) 50°C and (iii) 80°C to study the effect of higher temperatures found in the storage sites. Corrosion current, corrosion potential, anodic and cathodic Tafel slopes, polarization resistance and corrosion rates were obtained from electrochemical testing and evaluated. Increasing temperatures was found to lead to increasing corrosion rates for all samples. The data also revealed increased corrosion from sodium perrhenate on the mild steel A285 samples. The perrhenate anion (ReO4-) formed a redox couple with iron in the mild steel and accelerated metal dissolution that increased with temperature. Pitting and uniform corrosion was observed in the A285 and A537 mild steel samples. The A537 mild steel, however, displayed lower corrosion rates in the presence of perrhenate compared in the absence of perrhenate. A hypothesis has been proposed to explain the differences between the two alloys.

  15. Effect of long term chilled (up to 5weeks) then frozen (up to 12months) storage at two different sub-zero holding temperatures on beef: 1. Meat quality and microbial loads.

    PubMed

    Holman, Benjamin W B; Coombs, Cassius E O; Morris, Stephen; Kerr, Matthew J; Hopkins, David L

    2017-11-01

    Beef loins (LL) stored under different chilled-then-frozen storage combinations (up to 5 and 52weeks, respectively) and two frozen holding temperatures were evaluated for microbial load and meat quality parameters. We found holding temperature effects to be negligible, which suggest -12°C could deliver comparable quality LL to -18°C across these same storage periods. Meat quality parameters varied significantly, but when compared to existing consumer thresholds these may not be perceptible, colour being the exception which proved unacceptable, earlier into retail display when either chilled and subsequent frozen storage periods were increased. There was insufficient detection of key spoilage microbes to allow for statistical analysis, potentially due to the hygienic and commercially representative LL source, although variation in water activity, glycogen content, pH and other moisture parameters conducive to microbial proliferation were influenced by chilled-then-frozen storage. These outcomes could be applied to defining storage thresholds that assure beef quality within export networks, leveraging market access, and improving product management. Crown Copyright © 2017. Published by Elsevier Ltd. All rights reserved.

  16. Combined modified atmosphere packaging and low temperature storage delay lignification and improve the defense response of minimally processed water bamboo shoot.

    PubMed

    Song, Lili; Chen, Hangjun; Gao, Haiyan; Fang, Xiangjun; Mu, Honglei; Yuan, Ya; Yang, Qian; Jiang, Yueming

    2013-09-04

    Minimally processed water bamboo shoot (WBS) lignifies and deteriorates rapidly at room temperature, which limits greatly its marketability. This study was to investigate the effect of modified atmosphere packaging (MAP) on the sensory quality index, lignin formation, production of radical oxygen species (ROS) and activities of scavenging enzymes, membrane integrity and energy status of minimally processed WBS when packaged with or without the sealed low-density polyethylene (LDPE) bags, and then stored at 20°C for 9 days or 2°C for 60 days. The sensory quality of minimally processed WBS decreased quickly after 6 days of storage at 20°C. Low temperature storage maintained a higher sensory quality index within the first 30 days, but exhibited higher contents of lignin and hydrogen peroxide (H2O2) as compared with non-MAP shoots at 20°C. Combined MAP and low temperature storage not only maintained good sensory quality after 30 days, but also reduced significantly the increases in lignin content, superoxide anion (O2.-) production rate, H2O2 content and membrane permeability, maintained high activities of superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX), and reduced the increase in activities of lipase, phospholipase D (PLD) and lipoxygenase (LOX). Furthermore, the minimally processed WBS under MAP condition exhibited higher energy charge (EC) and lower adenosine monophosphate (AMP) content by the end of storage (60 days) at 2°C than those without MAP or stored for 9 days at 20°C. These results indicated that MAP in combination with low temperature storage reduced lignification of minimally processed WBS, which was closely associated with maintenance of energy status and enhanced activities of antioxidant enzymes, as well as reduced alleviation of membrane damage caused by ROS.

  17. Combined modified atmosphere packaging and low temperature storage delay lignification and improve the defense response of minimally processed water bamboo shoot

    PubMed Central

    2013-01-01

    Background Minimally processed water bamboo shoot (WBS) lignifies and deteriorates rapidly at room temperature, which limits greatly its marketability. This study was to investigate the effect of modified atmosphere packaging (MAP) on the sensory quality index, lignin formation, production of radical oxygen species (ROS) and activities of scavenging enzymes, membrane integrity and energy status of minimally processed WBS when packaged with or without the sealed low-density polyethylene (LDPE) bags, and then stored at 20°C for 9 days or 2°C for 60 days. Results The sensory quality of minimally processed WBS decreased quickly after 6 days of storage at 20°C. Low temperature storage maintained a higher sensory quality index within the first 30 days, but exhibited higher contents of lignin and hydrogen peroxide (H2O2) as compared with non-MAP shoots at 20°C. Combined MAP and low temperature storage not only maintained good sensory quality after 30 days, but also reduced significantly the increases in lignin content, superoxide anion (O2.-) production rate, H2O2 content and membrane permeability, maintained high activities of superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX), and reduced the increase in activities of lipase, phospholipase D (PLD) and lipoxygenase (LOX). Furthermore, the minimally processed WBS under MAP condition exhibited higher energy charge (EC) and lower adenosine monophosphate (AMP) content by the end of storage (60 days) at 2°C than those without MAP or stored for 9 days at 20°C. Conclusion These results indicated that MAP in combination with low temperature storage reduced lignification of minimally processed WBS, which was closely associated with maintenance of energy status and enhanced activities of antioxidant enzymes, as well as reduced alleviation of membrane damage caused by ROS. PMID:24006941

  18. Influence of Thawing Methods and Storage Temperatures on Bacterial Diversity, Growth Kinetics, and Biogenic Amine Development in Atlantic Mackerel.

    PubMed

    Onyango, S; Palmadottir, H; Tómason, T; Marteinsson, V T; Njage, P M K; Reynisson, E

    2016-11-01

    Limited knowledge is currently available on the influence of fish thawing and subsequent storage conditions on bacterial growth kinetics, succession, and diversity alongside the production of biogenic amines. This study aimed to address these factors during the thawing and subsequent storage of mackerel. Thawing was either done fast in 18°C water for 2 h or slowly at 30°C overnight. Subsequent storage was at 30°C (ambient) for 36 h and 2 to 5°C (refrigerated) for 12 days. The cultivation methods used were total viable counts, hydrogen sulfide-producing bacteria, and Pseudomonas . Maximum growth rate, population density, and lag time were fitted on the counts using the Baranyi model. The bacterial diversity and succession were based on sequencing of 16S rRNA amplicons, and biogenic amines were quantified on high-pressure liquid chromatography-UV. The results show that lag time of hydrogen sulfide-producing bacteria was significantly affected by both thawing methods, and further, the interaction between thawing and storage significantly affected the maximum growth rate of these bacteria. However, the maximum growth rate of Pseudomonas was higher during refrigerated storage compared with storage at ambient temperature. Total viable counts showed longer lag time and reduced growth rate under refrigerated storage. Higher bacterial diversity was correlated to slow thawing and storage at ambient temperature compared with slow thawing and refrigerated storage. Overall, Acinetobacter and Psychrobacter genera were the dominant bacterial populations. The amine levels were low and could not be differentiated along the thawing and storage approaches, despite a clear increase in bacterial load, succession, and diversity. This corresponded well with the low abundance of biogenic amine-producing bacteria, with the exception of the genus Proteus , which was 8.6% in fast-thawed mackerel during storage at ambient temperature. This suggests that the decarboxylation potential is dependent on both microbial load and microbial community structure.

  19. Hepatitis B vaccine freezing in the Indonesian cold chain: evidence and solutions.

    PubMed

    Nelson, Carib M; Wibisono, Hariadi; Purwanto, Hary; Mansyur, Isa; Moniaga, Vanda; Widjaya, Anton

    2004-02-01

    To document and characterize freezing temperatures in the Indonesian vaccine cold chain and to evaluate the feasibility of changes designed to reduce the occurrence of freezing. Data loggers were used to measure temperatures of shipments of hepatitis B vaccine from manufacturer to point of use. Baseline conditions and three intervention phases were monitored. During each of the intervention phases, vaccines were removed progressively from the standard 2-8 degrees C cold chain. Freezing temperatures were recorded in 75% of baseline shipments. The highest rates of freezing occurred during transport from province to district, storage in district-level ice-lined refrigerators, and storage in refrigerators in health centres. Interventions reduced freezing, without excessive heat exposure. Inadvertent freezing of freeze-sensitive vaccines is widespread in Indonesia. Simple strategies exist to reduce freezing - for example, selective transport and storage of vaccines at ambient temperatures. The use of vaccine vial monitors reduces the risk associated with heat-damaged vaccines in these scenarios. Policy changes that allow limited storage of freeze-sensitive vaccines at temperatures >2-8 degrees C would enable flexible vaccine distribution strategies that could reduce vaccine freezing, reduce costs, and increase capacity.

  20. Effect of water content on the glass transition temperature of mixtures of sugars, polymers, and penetrating cryoprotectants in physiological buffer.

    PubMed

    Drake, Andrew C; Lee, Youngjoo; Burgess, Emma M; Karlsson, Jens O M; Eroglu, Ali; Higgins, Adam Z

    2018-01-01

    Long-term storage of viable mammalian cells is important for applications ranging from in vitro fertilization to cell therapy. Cryopreservation is currently the most common approach, but storage in liquid nitrogen is relatively costly and the requirement for low temperatures during shipping is inconvenient. Desiccation is an alternative strategy with the potential to enable viable cell preservation at more convenient storage temperatures without the need for liquid nitrogen. To achieve stability during storage in the dried state it is necessary to remove enough water that the remaining matrix forms a non-crystalline glassy solid. Thus, the glass transition temperature is a key parameter for design of cell desiccation procedures. In this study, we have investigated the effects of moisture content on the glass transition temperature (Tg) of mixtures of sugars (trehalose or raffinose), polymers (polyvinylpyrrolidone or Ficoll), penetrating cryoprotectants (ethylene glycol, propylene glycol, or dimethyl sulfoxide), and phosphate buffered saline (PBS) solutes. Aqueous solutions were dried to different moisture contents by equilibration with saturated salt solutions, or by baking at 95°C. The glass transition temperatures of the dehydrated samples were then measured by differential scanning calorimetry. As expected, Tg increased with decreasing moisture content. For example, in a desiccation medium containing 0.1 M trehalose in PBS, Tg ranged from about 360 K for a completely dry sample to about 220 K at a water mass fraction of 0.4. Addition of polymers to the solutions increased Tg, while addition of penetrating cryoprotectants decreased Tg. Our results provide insight into the relationship between relative humidity, moisture content and glass transition temperature for cell desiccation solutions containing sugars, polymers and penetrating cryoprotectants.

  1. Effect of water content on the glass transition temperature of mixtures of sugars, polymers, and penetrating cryoprotectants in physiological buffer

    PubMed Central

    Burgess, Emma M.; Karlsson, Jens O. M.; Eroglu, Ali

    2018-01-01

    Long-term storage of viable mammalian cells is important for applications ranging from in vitro fertilization to cell therapy. Cryopreservation is currently the most common approach, but storage in liquid nitrogen is relatively costly and the requirement for low temperatures during shipping is inconvenient. Desiccation is an alternative strategy with the potential to enable viable cell preservation at more convenient storage temperatures without the need for liquid nitrogen. To achieve stability during storage in the dried state it is necessary to remove enough water that the remaining matrix forms a non-crystalline glassy solid. Thus, the glass transition temperature is a key parameter for design of cell desiccation procedures. In this study, we have investigated the effects of moisture content on the glass transition temperature (Tg) of mixtures of sugars (trehalose or raffinose), polymers (polyvinylpyrrolidone or Ficoll), penetrating cryoprotectants (ethylene glycol, propylene glycol, or dimethyl sulfoxide), and phosphate buffered saline (PBS) solutes. Aqueous solutions were dried to different moisture contents by equilibration with saturated salt solutions, or by baking at 95°C. The glass transition temperatures of the dehydrated samples were then measured by differential scanning calorimetry. As expected, Tg increased with decreasing moisture content. For example, in a desiccation medium containing 0.1 M trehalose in PBS, Tg ranged from about 360 K for a completely dry sample to about 220 K at a water mass fraction of 0.4. Addition of polymers to the solutions increased Tg, while addition of penetrating cryoprotectants decreased Tg. Our results provide insight into the relationship between relative humidity, moisture content and glass transition temperature for cell desiccation solutions containing sugars, polymers and penetrating cryoprotectants. PMID:29304068

  2. Role of blooming in determining the storage stability of lipid-based dosage forms.

    PubMed

    Khan, Nurzalina; Craig, Duncan Q M

    2004-12-01

    Gelucire 50/13 alone and solid dispersions in this material containing two model drugs (10% w/w caffeine and paracetamol) have been studied with a view to establishing the mechanism underpinning changes in drug-release characteristics as a function of storage time and temperature. The lipid systems were fabricated into tablets and stored for up to 180 days at temperatures of 20 and 37 degrees C. The dispersions were studied using differential scanning calorimetry (DSC), scanning electron microscopy, and dissolution testing. DSC studies indicated that the Gelucire 50/13 exists in two principal melting forms (melting points 38 and 43 degrees C) that undergo transformation to the higher melting form on storage at 37 degrees C. Scanning electron microscopy studies indicated that the systems exhibit "blooming," with crystal formation on the surface being apparent on storage at both temperatures. The dissolution rate increased on storage, with the effect being particularly marked at higher storage temperatures and for the paracetamol systems. However, whereas these changes corresponded well to those seen for the morphology, the correlation between the changes in dissolution and those of the DSC profiles was poor. The study has suggested a novel explanation for the storage instability of Gelucire 50/13 whereby the change in dissolution is associated not with molecular rearrangement as such but with the gross distribution of the constituent components, this in turn altering the physical integrity of the lipid bases. (c) 2004 Wiley-Liss, Inc. and the American Pharmacists Association

  3. Effect of storage temperature on endogenous GHB levels in urine.

    PubMed

    LeBeau, M A; Miller, M L; Levine, B

    2001-06-15

    Because gamma-hydroxybutyrate (GHB) is an endogenous substance present in the body and is rapidly eliminated after ingestion, toxicologists investigating drug-facilitated sexual assault cases are often asked to differentiate between endogenous and exogenous levels of GHB in urine samples. This study was designed to determine the effects of storage temperature on endogenous GHB levels in urine. Specifically, it was designed to ascertain whether endogenous levels can be elevated to a range considered indicative of GHB ingestion. Urine specimens from two subjects that had not been administered exogenous GHB were collected during a 24h period and individually pooled. The pooled specimens were separated into standard sample cups and divided into three storage groups: room temperature ( approximately 25 degrees C), refrigerated (5 degrees C), and frozen (-10 degrees C). Additionally, some specimens were put through numerous freeze/thaw cycles to mimic situations that may occur if multiple laboratories analyze the same specimen. Periodic analysis of the samples revealed increases in the levels of endogenous GHB over a 6-month period. The greatest increase (up to 404%) was observed in the samples maintained at room temperature. The refrigerated specimens showed increases of 140-208%, while the frozen specimens showed smaller changes (88-116%). The specimens subjected to multiple freeze/thaw cycles mirrored specimens that had been thawed only once. None of the stored urine specimens demonstrated increases in GHB concentrations that would be consistent with exogenous GHB ingestion.

  4. Stress relaxation in pre-stressed aluminum core–shell particles: X-ray diffraction study, modeling, and improved reactivity

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Levitas, Valery I.; McCollum, Jena; Pantoya, Michelle L.

    Stress relaxation in aluminum micron-scale particles covered by alumina shell after pre-stressing by thermal treatment and storage was measured using X-ray diffraction with synchrotron radiation. Pre-stressing was produced by annealing Al particles at 573K followed by fast cooling. While averaged dilatational strain in Al core was negligible for untreated particles, it was measured at 4.40×10 -5 and 2.85×10 -5 after 2 and 48 days of storage. Consistently, such a treatment leads to increase in flame propagation speed for Al+CuO mixture by 37% and 25%, respectively. Analytical model for creep in alumna shell and stress relaxation in Al core-alumina shellmore » structure is developed and activation energy and pre-exponential multiplier are estimated. The effect of storage temperature and annealing temperature on the kinetics of stress relaxation was evaluated theoretically. These results provide estimates for optimizing Al reactivity with the holding time at annealing temperature and allowable time for storage of Al particles for various environmental temperatures.« less

  5. Stress relaxation in pre-stressed aluminum core–shell particles: X-ray diffraction study, modeling, and improved reactivity

    DOE PAGES

    Levitas, Valery I.; McCollum, Jena; Pantoya, Michelle L.; ...

    2016-05-30

    Stress relaxation in aluminum micron-scale particles covered by alumina shell after pre-stressing by thermal treatment and storage was measured using X-ray diffraction with synchrotron radiation. Pre-stressing was produced by annealing Al particles at 573K followed by fast cooling. While averaged dilatational strain in Al core was negligible for untreated particles, it was measured at 4.40×10 -5 and 2.85×10 -5 after 2 and 48 days of storage. Consistently, such a treatment leads to increase in flame propagation speed for Al+CuO mixture by 37% and 25%, respectively. Analytical model for creep in alumna shell and stress relaxation in Al core-alumina shellmore » structure is developed and activation energy and pre-exponential multiplier are estimated. The effect of storage temperature and annealing temperature on the kinetics of stress relaxation was evaluated theoretically. These results provide estimates for optimizing Al reactivity with the holding time at annealing temperature and allowable time for storage of Al particles for various environmental temperatures.« less

  6. Relative importance of moisture migration and amylopectin retrogradation for pound cake crumb firming.

    PubMed

    Luyts, A; Wilderjans, E; Van Haesendonck, I; Brijs, K; Courtin, C M; Delcour, J A

    2013-12-15

    Moisture migration largely impacts cake crumb firmness during storage at ambient temperature. To study the importance of phenomena other than crumb to crust moisture migration and to exclude moisture and temperature gradients during baking, crustless cakes were baked using an electrical resistance oven (ERO). Cake crumb firming was evaluated by texture analysis. First, ERO cakes with properties similar to those baked conventionally were produced. Cake batter moisture content (MC) was adjusted to ensure complete starch gelatinisation in the baking process. In cakes baked conventionally, most of the increase in crumb firmness during storage was caused by moisture migration. Proton nuclear magnetic resonance ((1)H NMR) showed that the population containing protons of crystalline starch grew during cake storage. These and differential scanning calorimetry (DSC) data pointed to only limited amylopectin retrogradation. The limited increase in amylopectin retrogradation during cake storage cannot solely account for the significant firming of ERO cakes and, hence, other phenomena are involved in cake firming. Copyright © 2013 Elsevier Ltd. All rights reserved.

  7. Determination of trans- and cis-urocanic acid in relation to histamine, putrescine, and cadaverine contents in tuna (Auxis Thazard) at different storage temperatures.

    PubMed

    Zare, Davood; Muhammad, Kharidah; Bejo, Mohd Hair; Ghazali, H M

    2015-02-01

    Scombroid fish poisoning is usually associated with consumption of fish containing high levels of histamine. However, reports indicate that some cases have responded to antihistamine therapy while ingested histamine levels in these cases were low. Potentiation of histamine toxicity by some biogenic amines, and release of endogenous histamine by other compounds such as cis-urocanic acid (UCA) are some hypotheses that have been put forth to explain this anomaly. Very little is known about the effects of storage conditions on the production of both UCA isomers and biogenic amines in tuna. Thus, the production of trans- and cis-UCA, histamine, putrescine, and cadaverine in tuna during 15 d of storage at 0, 3, and 10 °C and 2 d storage at ambient temperature were monitored. The initial trans- and cis-UCA contents in fresh tuna were 2.90 and 1.47 mg/kg, respectively, whereas the levels of putrescine and cadaverine were less than 2 mg/kg, and histamine was not detected. The highest levels of trans- and cis-UCA were obtained during 15 d storage at 3 °C (23.74 and 21.79 mg/kg, respectively) while the highest concentrations of histamine (2796 mg/kg), putrescine (220.32 mg/kg) and cadaverine (1045.20 mg/kg) were obtained during storage at room temperature, 10 and 10 °C, respectively. Histamine content increased considerably during storage at 10 °C whereas trans- and cis-UCA contents changed slightly. The initial trans-UCA content decreased during storage at ambient temperature. Thus, unlike histamine, concentrations of trans- and cis-UCA did not result in elevated levels during storage of tuna. © 2015 Institute of Food Technologists®

  8. Survival of salmonella transformed to express green fluorescent protein on Italian parsley as affected by processing and storage.

    PubMed

    Duffy, E A; Cisneros-Zevallos, L; Castillo, A; Pillai, S D; Ricke, S C; Acuff, G R

    2005-04-01

    To study the effect of processing and storage parameters on the survival of Salmonella on fresh Italian parsley, parsley bunches were dipped for 3 or 15 min in suspensions that were preequilibrated to 5, 25, or 35 degrees C and inoculated with Salmonella transformed to express enhanced green fluorescent protein. Loosely attached and/or associated, strongly attached and/or associated, and internalized and/or entrapped Salmonella cells were enumerated over 0, 1, and 7 days of storage at 25 degrees C and over 0, 1, 7, 14, and 30 days of storage at 4 degrees C using surface-plating procedures. Leaf sections obtained from samples after 0, 1, and 7 days of storage were examined using confocal scanning laser microscopy. Temperature of the dip suspension had little effect on the attachment and survival of Salmonella cells on parsley. Regardless of the temperature or duration of dip, Salmonella was internalized. Immersion for longer times resulted in higher numbers of attached and internalized cells. Microscopic observations supported these results and revealed Salmonella cells near the stomata and within cracks in the cuticle. Storage temperature had the greatest impact on the survival of Salmonella cells on parsley. When stored at 25 degrees C, parsley had a shelf life of 7 days, and Salmonella populations significantly increased over the 7 days of storage. For parsley stored at 4 degrees C, numbers of Salmonella cells decreased over days 0, 1, and 7. After 7 days of storage, there were no viable internalized Salmonella cells detected. Storage temperature represents an important control point for the safety of fresh parsley.

  9. Prediction of microbial growth in fresh-cut vegetables treated with acidic electrolyzed water during storage under various temperature conditions.

    PubMed

    Koseki, S; Itoh, K

    2001-12-01

    Effects of storage temperature (1, 5, and 10 degrees C) on growth of microbial populations (total aerobic bacteria, coliform bacteria, Bacillus cereus, and psychrotrophic bacteria) on acidic electrolyzed water (AcEW)-treated fresh-cut lettuce and cabbage were determined. A modified Gompertz function was used to describe the kinetics of microbial growth. Growth data were analyzed using regression analysis to generate "best-fit" modified Gompertz equations, which were subsequently used to calculate lag time, exponential growth rate, and generation time. The data indicated that the growth kinetics of each bacterium were dependent on storage temperature, except at 1 degrees C storage. At 1 degrees C storage, no increases were observed in bacterial populations. Treatment of vegetables with AcEW produced a decrease in initial microbial populations. However, subsequent growth rates were higher than on nontreated vegetables. The recovery time required by the reduced microbial population to reach the initial (treated with tap water [TW]) population was also determined in this study, with the recovery time of the microbial population at 10 degrees C being <3 days. The benefits of reducing the initial microbial populations on fresh-cut vegetables were greatly affected by storage temperature. Results from this study could be used to predict microbial quality of fresh-cut lettuce and cabbage throughout their distribution.

  10. Preliminary Thermal Stress Analysis of a High-Pressure Cryogenic Storage Tank

    NASA Technical Reports Server (NTRS)

    Baker, J. Mark

    2003-01-01

    The thermal stresses on a cryogenic storage tank strongly affect the condition of the tank and its ability to withstand operational stresses. These thermal stresses also affect the growth of any surface damage that might occur in the tank walls. These stresses are particularly of concern during the initial cooldown period for a new tank placed into service, and during any subsequent thermal cycles. A preliminary thermal stress analysis of a high-pressure cryogenic storage tank was performed. Stresses during normal operation were determined, as well as the transient temperature distribution. An elastic analysis was used to determine the thermal stresses in the inner wall based on the temperature data. The results of this elastic analysis indicate that the inner wall of the storage tank will experience thermal stresses of approximately 145,000 psi (1000 MPa). This stress level is well above the room-temperature yield strength of 304L stainless steel, which is about 25,000 psi (170 MPa). For this preliminary analysis, several important factors have not yet been considered. These factors include increased strength of 304L stainless steel at cryogenic temperatures, plastic material behavior, and increased strength due to strain hardening. In order to more accurately determine the thermal stresses and their affect on the tank material, further investigation is required, particularly in the area of material properties and their relationship to stress.

  11. Improved boundary layer heat transfer calculations near a stagnation point

    NASA Technical Reports Server (NTRS)

    Ahn, Kyung Hwan

    1990-01-01

    A thermal design of a solar receiver has been developed for the solutions of problems involving phase-change thermal energy storage and natural convection loss. Two dimensional axisymmetrical solidification and melting of materials contained between two concentric cylinders of finite length has been studied for thermal energy storage analysis. For calculation of free convection loss inside receiver cavity, two dimensional axisymmetrical, laminar, transient free convection including radiation effects has been studied using integral/finite difference method. Finite difference equations are derived for the above analysis subject to constant or variable material properties, initial conditions, and boundary conditions. The validity of the analyses has been substantiated by comparing results of the present general method with available analytic solutions or numerical results reported in the literature. Both explicit and implicit schemes are tested in phase change analysis with different number of nodes ranging from 4 to 18. The above numerical methods have been applied to the existing solar receiver analyzing computer code as additional subroutines. The results were computed for one of the proposed Brayton cycle solar receiver models running under the actual environmental conditions. Effect of thermal energy storage on the thermal behavior of the receiver has been estimated. Due to the thermal energy storage, about 65% reduction on working gas outlet temperature fluctuation has been obtained; however, maximum temperature of thermal energy storage containment has been increased about 18%. Also, effect of natural convection inside a receiver cavity on the receiver heat transfer has been analyzed. The finding indicated that thermal stratification occurs during the sun time resulting in higher receiver temperatures at the outlet section of the gas tube, and lower temperatures at the inlet section of the gas tube when compared with the results with no natural convection. Due to heat supply from the air during the shade time, minimum temperature has been increased, while maximum temperature has been reduced due to convection loss to air. Consequently, cyclic temperature fluctuation has been reduced 29% for working gas and 16% for thermal energy storage containment. On the other hand, despite the presence of the natural convection the time-averaged temperatures for receiver components were found to be similar for two cases with/without natural convection (maximum difference was 1.8%).

  12. Technical challenges and future direction for high-efficiency metal hydride thermal energy storage systems

    NASA Astrophysics Data System (ADS)

    Ward, Patrick A.; Corgnale, Claudio; Teprovich, Joseph A.; Motyka, Theodore; Hardy, Bruce; Sheppard, Drew; Buckley, Craig; Zidan, Ragaiy

    2016-04-01

    Recently, there has been increasing interest in thermal energy storage (TES) systems for concentrated solar power (CSP) plants, which allow for continuous operation when sunlight is unavailable. Thermochemical energy storage materials have the advantage of much higher energy densities than latent or sensible heat materials. Furthermore, thermochemical energy storage systems based on metal hydrides have been gaining great interest for having the advantage of higher energy densities, better reversibility, and high enthalpies. However, in order to achieve higher efficiencies desired of a thermal storage system by the US Department of Energy, the system is required to operate at temperatures >600 °C. Operation at temperatures >600 °C presents challenges including material selection, hydrogen embrittlement and permeation of containment vessels, appropriate selection of heat transfer fluids, and cost. Herein, the technical difficulties and proposed solutions associated with the use of metal hydrides as TES materials in CSP applications are discussed and evaluated.

  13. Oxygen- and Lithium-Doped Hybrid Boron-Nitride/Carbon Networks for Hydrogen Storage.

    PubMed

    Shayeganfar, Farzaneh; Shahsavari, Rouzbeh

    2016-12-20

    Hydrogen storage capacities have been studied on newly designed three-dimensional pillared boron nitride (PBN) and pillared graphene boron nitride (PGBN). We propose these novel materials based on the covalent connection of BNNTs and graphene sheets, which enhance the surface and free volume for storage within the nanomaterial and increase the gravimetric and volumetric hydrogen uptake capacities. Density functional theory and molecular dynamics simulations show that these lithium- and oxygen-doped pillared structures have improved gravimetric and volumetric hydrogen capacities at room temperature, with values on the order of 9.1-11.6 wt % and 40-60 g/L. Our findings demonstrate that the gravimetric uptake of oxygen- and lithium-doped PBN and PGBN has significantly enhanced the hydrogen sorption and desorption. Calculations for O-doped PGBN yield gravimetric hydrogen uptake capacities greater than 11.6 wt % at room temperature. This increased value is attributed to the pillared morphology, which improves the mechanical properties and increases porosity, as well as the high binding energy between oxygen and GBN. Our results suggest that hybrid carbon/BNNT nanostructures are an excellent candidate for hydrogen storage, owing to the combination of the electron mobility of graphene and the polarized nature of BN at heterojunctions, which enhances the uptake capacity, providing ample opportunities to further tune this hybrid material for efficient hydrogen storage.

  14. Temperature-dependent dielectric and energy-storage properties of Pb(Zr,Sn,Ti)O{sub 3} antiferroelectric bulk ceramics

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Chen, Xuefeng; Liu, Zhen; Xu, Chenhong

    2016-05-15

    The dielectric and energy-storage properties of Pb{sub 0.99}Nb{sub 0.02}[(Zr{sub 0.60}Sn{sub 0.40}){sub 0.95}Ti{sub 0.05}]{sub 0.98}O{sub 3} (PNZST) bulk ceramics near the antiferroelectric (AFE)-ferroelectric (FE) phase boundary are investigated as a function of temperature. Three characteristic temperatures T{sub 0}, T{sub C}, T{sub 2} are obtained from the dielectric temperature spectrum. At different temperature regions (below T{sub 0}, between T{sub 0} and T{sub C}, and above T{sub C}), three types of hysteresis loops are observed as square double loop, slim loop and linear loop, respectively. The switching fields and recoverable energy density all first increase and then decrease with increasing temperature, and reachmore » their peak values at ∼T{sub 0}. These results provide a convenient method to optimize the working temperature of antiferroelectric electronic devices through testing the temperature dependent dielectric properties of antiferroelectric ceramics.« less

  15. Synthesis of Ni/Graphene Nanocomposite for Hydrogen Storage.

    PubMed

    Zhou, Chunyu; Szpunar, Jerzy A; Cui, Xiaoyu

    2016-06-22

    We have designed a Ni-graphene composite for hydrogen storage with Ni nanoparticles of 10 nm in size, uniformly dispersed over a graphene substrate. This system exhibits attractive features like high gravimetric density, ambient conditions, and low activation temperature for hydrogen release. When charged at room temperature and an atmospheric hydrogen pressure of 1 bar, it could yield a hydrogen capacity of 0.14 wt %. When hydrogen pressure increased to 60 bar, the sorbent had a hydrogen gravimetric density of 1.18 wt %. The hydrogen release could occur at an operating temperature below 150 °C and completes at 250 °C.

  16. Development and Performance Evaluation of High Temperature Concrete for Thermal Energy Storage for Solar Power Generation

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    R. Panneer Selvam; Hale, Micah; Strasser, Matt

    Thermal energy can be stored by the mechanism of sensible or latent heat or heat from chemical reactions. Sensible heat is the means of storing energy by increasing the temperature of the solid or liquid. Since the concrete as media cost per kWh thermal is $1, this seems to be a very economical material to be used as a TES. This research is focused on extending the concrete TES system for higher temperatures (500 °C to 600 °C) and increasing the heat transfer performance using novel construction techniques. To store heat at high temperature special concretes are developed and testedmore » for its performance. The storage capacity costs of the developed concrete is in the range of $0.91-$3.02/kWh thermal. Two different storage methods are investigated. In the first one heat is transported using molten slat through a stainless steel tube and heat is transported into concrete block through diffusion. The cost of the system is higher than the targeted DOE goal of $15/kWht hermal. The increase in cost of the system is due to stainless steel tube to transfer the heat from molten salt to the concrete blocks.The other method is a one-tank thermocline system in which both the hot and cold fluid occupy the same tank resulting in reduced storage tank volume. In this model, heated molten salt enters the top of the tank which contains a packed bed of quartzite rock and silica sand as the thermal energy storage (TES) medium. The single-tank storage system uses about half the salt that is required by the two-tank system for a required storage capacity. This amounts to a significant reduction in the cost of the storage system. The single tank alternative has also been proven to be cheaper than the option which uses large concrete modules with embedded heat exchangers. Using computer models optimum dimensions are determined to have an round trip efficiency of 84%. Additionally, the cost of the structured concrete thermocline configuration provides the TES capacity cost of $33.80$/kWh thermal compared with $30.04/kWhthermal for a packed-bed thermocline (PBTC) configuration and $46.11/kWh thermal for a two-tank liquid configuration.« less

  17. Effects of field storage method on E. coli concentrations measured in storm water runoff.

    PubMed

    Harmel, Daren; Wagner, Kevin; Martin, Emily; Smith, Doug; Wanjugi, Pauline; Gentry, Terry; Gregory, Lucas; Hendon, Tina

    2016-03-01

    Storm water runoff is increasingly assessed for fecal indicator organisms (e.g., Escherichia coli, E. coli) and its impact on contact recreation. Concurrently, use of autosamplers along with logistic, economic, technical, and personnel barriers is challenging conventional protocols for sample holding times and storage conditions in the field. A common holding time limit for E. coli is 8 h with a 10 °C storage temperature, but several research studies support longer hold time thresholds. The use of autosamplers to collect E. coli water samples has received little field research attention; thus, this study was implemented to compare refrigerated and unrefrigerated autosamplers and evaluate potential E. coli concentration differences due to field storage temperature (storms with holding times ≤24 h) and due to field storage time and temperature (storms >24 h). Data from 85 runoff events on four diverse watersheds showed that field storage times and temperatures had minor effects on mean and median E. coli concentrations. Graphs and error values did, however, indicate a weak tendency for higher concentrations in the refrigerated samplers, but it is unknown to what extent differing die-off and/or regrowth rates, heterogeneity in concentrations within samples, and laboratory analysis uncertainty contributed to the results. The minimal differences in measured E. coli concentrations cast doubt on the need for utilizing the rigid conventional protocols for field holding time and storage temperature. This is not to say that proper quality assurance and quality control is not important but to emphasize the need to consider the balance between data quality and practical constraints related to logistics, funding, travel time, and autosampler use in storm water studies.

  18. Tailoring Thermodynamics and Kinetics for Hydrogen Storage in Complex Hydrides towards Applications.

    PubMed

    Liu, Yongfeng; Yang, Yaxiong; Gao, Mingxia; Pan, Hongge

    2016-02-01

    Solid-state hydrogen storage using various materials is expected to provide the ultimate solution for safe and efficient on-board storage. Complex hydrides have attracted increasing attention over the past two decades due to their high gravimetric and volumetric hydrogen densities. In this account, we review studies from our lab on tailoring the thermodynamics and kinetics for hydrogen storage in complex hydrides, including metal alanates, borohydrides and amides. By changing the material composition and structure, developing feasible preparation methods, doping high-performance catalysts, optimizing multifunctional additives, creating nanostructures and understanding the interaction mechanisms with hydrogen, the operating temperatures for hydrogen storage in metal amides, alanates and borohydrides are remarkably reduced. This temperature reduction is associated with enhanced reaction kinetics and improved reversibility. The examples discussed in this review are expected to provide new inspiration for the development of complex hydrides with high hydrogen capacity and appropriate thermodynamics and kinetics for hydrogen storage. © 2015 The Chemical Society of Japan & Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

  19. High-throughput metataxonomic characterization of the raw milk microbiota identifies changes reflecting lactation stage and storage conditions.

    PubMed

    Doyle, Conor J; Gleeson, David; O'Toole, Paul W; Cotter, Paul D

    2017-08-16

    Low temperature is used to control the growth of bacteria in milk, both pre- and post-pasteurization. As the duration of refrigerated storage extends, psychrotrophs dominate the milk microbiota, that can produce heat stable lipases which negatively impact the organoleptic qualities of milk. Here we examine the influence that refrigeration temperature (2°C, 4°C and 6°C) and storage duration (96h) have on the microbiota composition (16S profiling) of raw bulk tank milk (BTM). To reflect a proposed change to current farming practices, raw milk was blended after each milking (8 milkings) and stored for five consecutive days in each temperature-specific tank. Here 16S rRNA-based microbiota compositional analysis was performed after milk was collected on day 1 and again after the final addition of milk at day 5. In addition to assessing the impact of the duration and temperature of storage, the influence of lactation stage, i.e. mid- versus late-lactation, on the microbiota of the blended BTM was also examined. Overall, both temperature and length of storage had surprisingly little influence on the raw milk microbiota, other than an increase in proportions of Gammaproteobacteria in the blended milk samples collected after pooling on day 5, and in samples stored at 6°C. However, lactation stage had a considerable influence on microbiota composition, with milk from mid-lactation containing higher proportions of Bacteroides, Faecalibacterium, Campylobacter and Rhodanobacter, and late-lactation milk containing higher proportions of Actinobacteria. Overall, the study demonstrates that current temperature and storage duration practises impact the microbiota of raw milk, but these impacts are modest relative to the more considerable differences between mid and late-lactation milk. Copyright © 2017 Elsevier B.V. All rights reserved.

  20. Performance of raw bovine meat preservation by hyperbaric storage (quasi energetically costless) compared to refrigeration.

    PubMed

    Freitas, Paulo; Pereira, Sofia A; Santos, Mauro D; Alves, Susana P; Bessa, Rui J B; Delgadillo, Ivonne; Saraiva, Jorge A

    2016-11-01

    Hyperbaric storage at room temperature (without temperature control) of raw bovine meat was studied and compared to refrigeration. Samples were first stored for 12h at 50, 100 and 150MPa, and in a second set of experiments, for a longer period of 10days at 50MPa. For the 12h storage, refrigeration and 50MPa had a similar microbial growth inhibition effect and, at 100 and 150MPa an additional microbial inactivation effect was found. For the longer experiment (10days at 50MPa) results pointed for a shelf-life increase of raw beef compared to samples stored under refrigeration. For both tests (12h and 10days) samples preserved under pressure showed no detrimental effect on physicochemical parameters comparatively to the initial and refrigerated samples. These results indicate that hyperbaric storage at room temperature not only allows high energy savings, but additionally has potential to extend the shelf-life of a perishable food product compared to refrigeration. Copyright © 2016 Elsevier Ltd. All rights reserved.

  1. Temperature of frozen storage affects the nature and consequences of protein oxidation in beef patties.

    PubMed

    Utrera, Mariana; Morcuende, David; Estévez, Mario

    2014-03-01

    The effect of three frozen storage temperatures (-8, -18 and -80 °C) on protein oxidation in beef patties was studied through the analysis of novel oxidation markers. Additionally, the connection between lipid and protein oxidation and the impact of the latter on particular quality traits (water holding capacity, color and texture) of subsequently processed beef patties (cooking/cold-stored) were investigated. Protein oxidation was measured as the loss of tryptophan fluorescence and formation of diverse lysine oxidation products (α-aminoadipic semialdehyde, α-aminoadipic acid and Schiff bases). Lipid oxidation was assessed by levels of thiobarbituric acid reactive substances and hexanal. A significant effect of storage temperature on protein oxidation was detected. Frozen storage increased the susceptibility of meat proteins to undergo further oxidation during processing. Timely interactions were found between lipid and protein oxidation. Plausible mechanisms by which oxidative damage to proteins may have an impact in particular quality traits are thoroughly discussed. © 2013.

  2. Metabolic costs of capital energy storage in a small-bodied ectotherm.

    PubMed

    Griffen, Blaine D

    2017-04-01

    Reproduction is energetically financed using strategies that fall along a continuum from animals that rely on stored energy acquired prior to reproduction (i.e., capital breeders) to those that rely on energy acquired during reproduction (i.e., income breeders). Energy storage incurs a metabolic cost. However, previous studies suggest that this cost may be minimal for small-bodied ectotherms. Here I test this assumption. I use a laboratory feeding experiment with the European green crab Carcinus maenas to establish individuals with different amounts of energy storage. I then demonstrate that differences in energy storage account for 26% of the variation in basal metabolic costs. The magnitudes of these costs for any individual crab vary through time depending on the amount of energy it has stored, as well as on temperature-dependent metabolism. I use previously established relationships between temperature- and mass-dependent metabolic rates, combined with a feasible annual pattern of energy storage in the Gulf of Maine and annual sea surface temperature patterns in this region, to estimate potential annual metabolic costs expected for mature female green crabs. Results indicate that energy storage should incur an ~8% increase in metabolic costs for female crabs, relative to a hypothetical crab that did not store any energy. Translated into feeding, for a medium-sized mature female (45 mm carapace width), this requires the consumption of an additional ~156 mussels annually to support the metabolic cost of energy storage. These results indicate, contrary to previous assumptions, that the cost of energy storage for small-bodied ectotherms may represent a considerable portion of their basic operating energy budget. An inability to meet these additional costs of energy storage may help explain the recent decline of green crabs in the Gulf of Maine where reduced prey availability and increased consumer competition have combined to hamper green crab foraging success in recent years.

  3. Storage of breast milk: effect of temperature and storage duration on microbial growth.

    PubMed

    Igumbor, E O; Mukura, R D; Makandiramba, B; Chihota, V

    2000-09-01

    To study the effect of storage duration at varying temperature ranges, the pattern of microbial isolates and the quantity of colony-forming units (CFU) on expressed breast milk. Cross sectional study. Bacteriology laboratory, University of Zimbabwe in Parirenyatwa Hospital, Harare. The temperature, storage duration and types of micro-organisms in freshly expressed breast milk. Freshly expressed human breast milk contained microbial non-pathogens of skin flora. There was no growth of organisms in stored breast milk after four hours, eight hours, 24 hours and 72 hours storage duration at temperature ranges 0 to 4 degrees C (freezing temperature), 4 to 10 degrees C (refrigerator temperature), 15 to 27 degrees C (room temperature) and 30 to 38 degrees C (high temperature) respectively. Growth was detected after the storage durations and organisms isolated were both pathogens and non-pathogens with low counts. Average colony counts was (CFU < 200). The study revealed that storage duration for expressed breast milk should not exceed 24 hours in refrigerator temperature (4 to 10 degrees C), eight hours at room temperature (15 to 27 degrees C) and four hours at high temperature (30 to 38 degrees C). Although freezing temperature (0 to 4 degrees C) seemed safest for breast milk storage, short-term storage in a freezer is not recommended due to likely the hazards of the thawing process.

  4. Warming-related increases in soil CO2 efflux are explained by increased below-ground carbon flux

    Treesearch

    Christian P. Giardina; Creighton M. Litton; Susan E. Crow; Gregory P Asner

    2014-01-01

    The universally observed exponential increase in soil-surface CO2 effux (‘soil respiration’; FS) with increasing temperature has led to speculation that global warming will accelerate soil organic carbon (SOC) decomposition, reduce SOC storage, and drive a positive feedback to future warming. However, interpreting temperature–FS relationships,...

  5. Dielectric and Energy Storage Properties of the Heterogeneous P(VDF-HFP)/PC Composite Films

    NASA Astrophysics Data System (ADS)

    Zhao, Xiaojia; Peng, Guirong; Zhan, Zaiji

    2017-12-01

    Polymer-based materials with a high discharge energy and low energy loss have attracted considerable attention for energy storage applications. A new class of polymer-based composite films composed of amorphous polycarbonate (PC) and poly(vinylidene fluoride-hexafluoropropylene) [P(VDF-HFP)] has been fabricated by simply solution blending followed by thermal treatment under vacuum. The results show that the diameter of the spherical phase for PC and the melting temperature of P(VDF-HFP) increase, and the crystallinity and crystallization temperature of P(VDF-HFP) decrease with increasing PC content. The phase transition from the polar β phase to weak polarity γ phase is induced by PC addition. Moreover, the Curie temperature of the P(VDF-HFP)/PC composite films shifts to a lower temperature. With the addition of PC, the permittivity, polarization and discharge energy of the P(VDF-HFP)/PC composite films slightly decrease. However, the energy loss is significantly reduced.

  6. Statistical Analysis of Terrestrial Water Storage Change Over Southwestern United States

    NASA Astrophysics Data System (ADS)

    Eibedingil, I. G.; Mubako, S. T.; Hargrove, W. L.; Espino, A. C.

    2017-12-01

    A warming trend over recent decades has aggravated water resource challenges in the arid southwestern region of the United States (U.S.). An increase in temperature, coupled with decreasing snowpack and rainfall have impacted the region's cities, ecosystems, and agriculture. The region is the largest contributor of agricultural products to the U.S. market resulting from irrigation. Water use through irrigation is stressing already limited terrestrial water resources. Population growth in recent decades has also led to increased water demand. This study utilizes products of the Gravity Recovery and Climate Experiment (GRACE) twin satellites experiment in MATLAB and ArcGIS to examine terrestrial water storage changes in the southwestern region of the U.S., comprised of the eight states of Texas, California, Nevada, Utah, Arizona, Colorado, New Mexico, and Oklahoma. Linear trend analysis was applied to the equivalent water-height data of terrestrial water storage changes (TWSC), precipitation, and air temperature. Correlation analysis was performed on couplings of TWSC - precipitation and TWSC - air temperature to examine the impact of temperature and precipitation on the region's water resources. Our preliminary results show a decreasing trend of TWSC from April 2002 to July 2016 in almost all parts of the region. Precipitation shows a decreasing trend from March 2000 to March 2017 for most of the region, except for sparse areas of increased precipitation near the northwestern coast of California, and a belt running from Oklahoma through the middle of Texas to the El Paso/New Mexico border. From April 2002 to December 2014, air temperature exhibited a negative trend for most of the region, except a larger part of California and a small location in central Texas. Correlation between TWSC and precipitation was mostly positive, but a negative trend was observed when TWSC and air temperature were correlated. The study contributes to the understanding of terrestrial water storage trends and their relationship with climatic variables, crucial for implementing appropriate adaptation and mitigation policies and strategies, and managing water demand.

  7. [Collagenolytic activity in several species of deuteromycetes under various storage conditions].

    PubMed

    Iakovleva, M B; Khoang, T L; Nikitina, Z K

    2006-01-01

    The ability of deuteromycetes of the genera Penicillium, Aspergillus, and Botrytis to retain collagenolytic activity was studied after both 2 and 10 years of storage on a Czapek medium under a layer of mineral oil at 4 degrees C, as well as in silica gel granules at 20 and -60 degrees C. The enzymatic activity of several species, including Botrytis terrestris, Penicillium janthinellum, Penicillium chrysogenum, and Penicillium citrinum, was retained under both conditions of storage. Aspergillus repens retained enzymatic activity only if stored under a layer of mineral oil. The viability of conidia and the collagenolytic activity of Botrytis terrestris, P. janthinellum, P. chrysogenum, and Penicillium citrinum, maintained on silica gel for 10 years, depended on the storage temperature. The viability of the test strains improved after storage on a silica gel at -60 degrees C. A strain of Aspergillus repens lost its ability to dissolve collagen at various storage temperatures on the silica gel. The index of lysis for three strains of Penicillium deuteromycetes (Penicillium janthinellum, Penicillium chrysogenum, and Penicillium citrinum) increased after a 10-year storage on silica gel at -60 degrees C.

  8. Applications and challenges for thermal energy storage

    NASA Astrophysics Data System (ADS)

    Kannberg, L. D.; Tomlinson, J. T.

    1991-04-01

    New thermal energy storage (TES) technologies are being developed and applied as society strives to relieve increasing energy and environmental stresses. Applications for these new technologies range from residential and district heating and cooling using waste and solar energy, to high-temperature energy storage for power production and industrial processes. In the last two decades there has been great interest and development of heat storage systems, primarily for residential and commercial buildings. While development has continued, the rate of advancement has slowed with current technology considered adequate for electrically charged heat storage furnaces. Use of chill storage for building diurnal cooling has received substantial development.

  9. Effects of ZnO nanoparticle-coated packaging film on pork meat quality during cold storage.

    PubMed

    Suo, Biao; Li, Huarong; Wang, Yuexia; Li, Zhen; Pan, Zhili; Ai, Zhilu

    2017-05-01

    There has been limited research on the use of ZnO nanoparticle-coated film for the quality preservation of pork meat under low temperature. In the present study, ZnO nanoparticles were mixed with sodium carboxymethyl cellulose (CMC-Na) to form a nanocomposite film, to investigate the effect of ZnO nanoparticle-coated film on pork meat quality and the growth of bacteria during storage under low temperature. When ZnO nanoparticle-coated film was used as the packaging material for pork meat for 14 days of cold storage at 4 °C, the results demonstrated a significant effect on restricting the increases in total volatile basic nitrogen and pH levels, limiting the decreases of lightness (increased L* value) and redness (increased a* value), and maintaining the water-holding capacity compared to the control pork samples (P < 0.05). The present study also discovered that the ZnO nanoparticle-coated film restrained the increase in total plate count (TPC). When Staphylococcus aureus was used as the representative strain, scanning electron microscopy revealed that ZnO nanoparticles increased the occurrence of cell membrane rupture under cold conditions. ZnO nanoparticle-coated film helps retain the quality of pork meat during cold storage by increasing the occurrence of microorganism injury. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry.

  10. Sugar metabolism, chip color, invertase activity, and gene expression during long-term cold storage of potato (Solanum tuberosum) tubers from wild-type and vacuolar invertase silencing lines of Katahdin.

    PubMed

    Wiberley-Bradford, Amy E; Busse, James S; Jiang, Jiming; Bethke, Paul C

    2014-11-16

    Storing potato tubers at low temperatures minimizes sprouting and disease but can cause an accumulation of reducing sugars in a process called cold-induced sweetening. Tubers with increased amounts of reducing sugars produce dark-colored, bitter-tasting fried products with elevated amounts of acrylamide, a possible carcinogen. Vacuolar invertase (VInv), which converts sucrose produced by starch breakdown to glucose and fructose, is the key determinant of reducing sugar accumulation during cold-induced sweetening. In this study, wild-type tubers and tubers in which VInv expression was reduced by RNA interference were used to investigate time- and temperature-dependent changes in sugar contents, chip color, and expression of VInv and other genes involved in starch metabolism in tubers during long-term cold storage. VInv activities and tuber reducing sugar contents were much lower, and tuber sucrose contents were much higher, in transgenic than in wild-type tubers stored at 3-9°C for up to eight months. Large differences in VInv mRNA accumulation were not observed at later times in storage, especially at temperatures below 9°C, so differences in invertase activity were likely established early in the storage period and maintained by stability of the invertase protein. Sugar contents, chip color, and expression of several of the studied genes, including AGPase and GBSS, were affected by storage temperature in both wild-type and transgenic tubers. Though transcript accumulation for other sugar-metabolism genes was affected by storage temperature and duration, it was essentially unaffected by invertase silencing and altered sugar contents. Differences in stem- and bud-end sugar contents in wild-type and transgenic tubers suggested different compartmentalization of sucrose at the two ends of stored tubers. VInv silencing significantly reduced cold-induced sweetening in stored potato tubers, likely by means of differential VInv expression early in storage. Transgenic tubers retained sensitivity to storage temperature, and accumulated greater amounts of sucrose, glucose and fructose at 3°C than at 7-9°C. At each storage temperature, suppression of VInv expression and large differences in tuber sugar contents had no effect on expression of AGPase and GBSS, genes involved in starch metabolism, suggesting that transcription of these genes is not regulated by tuber sugar content.

  11. Predicting possible effects of H2S impurity on CO2 transportation and geological storage.

    PubMed

    Ji, Xiaoyan; Zhu, Chen

    2013-01-02

    For CO(2) geological storage, permitting impurities, such as H(2)S, in CO(2) streams can lead to a great potential for capital and energy savings for CO(2) capture and separation, but it also increases costs and risk management for transportation and storage. To evaluate the cost-benefits, using a recently developed model (Ji, X.; Zhu, C. Geochim. Cosmochim. Acta 2012, 91, 40-59), this study predicts phase equilibria and thermodynamic properties of the system H(2)S-CO(2)-H(2)O-NaCl under transportation and storage conditions and discusses potential effects of H(2)S on transportation and storage. The prediction shows that inclusion of H(2)S in CO(2) streams may lead to two-phase flow. For H(2)S-CO(2) mixtures, at a given temperature, the bubble and dew pressures decrease with increasing H(2)S content, while the mass density increases at low pressures and decreases at high pressures. For the CO(2)-H(2)S-H(2)O system, the total gas solubility increases while the mass density of the aqueous solution with dissolved gas decreases. For the CO(2)-H(2)S-H(2)O-NaCl system, at a given temperature, pressure and NaCl concentration, the solubility of the gas mixture in aqueous phase increases with increasing H(2)S content and then decreases, while the mass density of aqueous solution decreases and may be lower than the mass density of the solution without gas dissolution.

  12. Stability of 21 Cocaine, Opioid and Benzodiazepine Drug Analytes in Spiked Meconium at Three Temperatures.

    PubMed

    Wu, Fang; Marin, Stephanie J; McMillin, Gwendolyn A

    2017-04-01

    In this study, the stability of 21 cocaine, opioid and benzodiazepine analytes in spiked meconium was investigated at three storage temperatures: 4°C, room temperature (RT), and 37°C (body temperature). The drugs/metabolites included were hydrocodone, hydromorphone, codeine, morphine, 6-acetylmorphine (6-AM), oxycodone, oxymorphone, cocaine, cocaethylene, benzoylecgonine, m-hydroxybenzoylecgonine, diazepam, oxazepam, temazepam, nordiazepam, chlordiazepoxide, lorazepam, alprazolam, alpha-hydroxyalprazolam, clonazepam, 7-aminoclonazepam, midazolam, alpha-hydroxymidazolam and zolpidem. Drug testing was performed using mass spectrometry methods that were validated for clinical use. After 2 weeks of storage, a substantial loss was observed in the concentrations of 7-aminoclonazepam (48.4% at 4°C and 71.5% at RT), and chlordiazepoxide (59.5% at RT). A slight decrease was observed in the concentrations of alprazolam (20.9% at 4°C), clonazepam (24.5% at 4°C), chlordiazepoxide (23.5% at 4°C), midazolam (20.8% at 4°C), nordiazepam (22.8% at RT), and alpha-hydroxyalprazolam (20.7% at 4°C). At 37°C, the concentrations of chlordiazepoxide, 7-aminoclonazepam, lorazepam, oxazepam, nordiazepam and temazepam decreased by 81.4%, 86.8%, 56.5%, 59.9%, 45.4% and 31.7%, respectively, after 2 weeks. 6-AM was observed to be unstable regardless of storage temperatures. For morphine, a 33.3% increase at 4°C and a 23.4% increase at RT were observed after 2 weeks, respectively, possibly due to 6-AM degradation, while no changes ≥20% were observed at 37°C. All other analytes were stable up to 2 weeks at all three storage temperatures (concentration changes <20%). The stability of select drug analytes in authentic clinical meconium specimens was consistent with that observed in spiked meconium. In conclusion, some drugs in meconium may not be stable for long periods of time. Sample storage conditions are an important consideration in the context of detection windows and interpreting drug-testing results in meconium. To the best of our knowledge, this is the first stability study of cocaine, opioids and benzodiazepines in meconium concerning the effects of storage temperatures. © The Author 2016. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  13. Thermal energy storage for low grade heat in the organic Rankine cycle

    NASA Astrophysics Data System (ADS)

    Soda, Michael John

    Limits of efficiencies cause immense amounts of thermal energy in the form of waste heat to be vented to the atmosphere. Up to 60% of unrecovered waste heat is classified as low or ultra-low quality, making recovery difficult or inefficient. The organic Rankine cycle can be used to generate mechanical power and electricity from these low temperatures where other thermal cycles are impractical. A variety of organic working fluids are available to optimize the ORC for any target temperature range. San Diego State University has one such experimental ORC using R245fa, and has been experimenting with multiple expanders. One limitation of recovering waste heat is the sporadic or cyclical nature common to its production. This inconsistency makes sizing heat recovery ORC systems difficult for a variety of reasons including off-design-point efficiency loss, increased attrition from varying loads, unreliable outputs, and overall system costs. Thermal energy storage systems can address all of these issues by smoothing the thermal input to a constant and reliable level and providing back-up capacity for times when the thermal input is deactivated. Multiple types of thermal energy storage have been explored including sensible, latent, and thermochemical. Latent heat storage involves storing thermal energy in the reversible phase change of a phase change material, or PCM, and can have several advantages over other modalities including energy storage density, cost, simplicity, reliability, relatively constant temperature output, and temperature customizability. The largest obstacles to using latent heat storage include heat transfer rates, thermal cycling stability, and potentially corrosive PCMs. Targeting 86°C, the operating temperature of SDSU's experimental ORC, multiple potential materials were explored and tested as potential PCMs including Magnesium Chloride Hexahydrate (MgCl2˙6H2O), Magnesium Nitrate Hexahydrate (Mg(NO3)2˙6H 2O), montan wax, and carnauba wax. The addition of graphite to augment heat transfer rates was also tested. Melting and solidification temperatures largely matched predictions. The magnesium salts were found to be less stable under thermal cycling than the waxes. Graphite was only soluble in the waxes. Mixtures of magnesium salts and waxes yielded a layered composite with the less dense waxes creating a sealing layer over the salt layer that significantly increased the stability of the magnesium salts. Research into optimum heat exchangers and storage vessels for these applications indicates that horizontally oriented aluminum pipes with vertically oriented aluminum fins would be the best method of storing and retrieving energy. Fin spacing can be predicted by an equation based on target temperatures and PCM characteristics.

  14. Heat production and storage are positively correlated with measures of body size/composition and heart rate drift during vigorous running.

    PubMed

    Buresh, Robert; Berg, Kris; Noble, John

    2005-09-01

    The purposes of this study were to determine the relationships between: (a) measures of body size/composition and heat production/storage, and (b) heat production/storage and heart rate (HR) drift during running at 95% of the velocity that elicited lactate threshold, which was determined for 20 healthy recreational male runners. Subsequently, changes in skin and tympanic temperatures associated with a vigorous 20-min run, HR, and VO2 data were recorded. It was found that heat production was significantly correlated with body mass (r = .687), lean mass (r = .749), and body surface area (BSA, r = .699). Heat storage was significantly correlated with body mass (r = .519), fat mass (r = .464), and BSA (r = .498). The percentage of produced heat stored was significantly correlated with body mass (r = .427), fat mass (r = .455), and BSA (r = .414). Regression analysis showed that the sum of body mass, percentage of body fat, BSA, lean mass, and fat mass accounted for 30% of the variability in heat storage. It was also found that HR drift was significantly correlated with heat storage (r = .383), percentage of produced heat stored (r = .433), and core temperature change (r = .450). It was concluded that heavier runners experienced greater heat production, heat storage, and core temperature increases than lighter runners during vigorous running.

  15. Sweet cherry softening accompanied with moisture migration and loss during low-temperature storage.

    PubMed

    Zhu, Danshi; Liang, Jieyu; Liu, He; Cao, Xuehui; Ge, Yonghong; Li, Jianrong

    2018-08-01

    Hardness is one of the important qualities influencing consumer appeal and marketing of fresh sweet cherry (Prunus avium L.). Moisture loss is one of the main causative factors of cherry softening. In this work, moisture loss and softening process of sweet cherry during postharvest storage at 0 and 4 °C were studied. In addition, low-field 1 H nuclear magnetic resonance (LF-NMR) was used to analyze water distribution and migration in sweet cherry during storage at 4 °C. Moisture content correlated significantly (p < 0.01) with both skin and flesh hardness of cherry fruit at the two storage temperatures. According to the transverse relaxation curve, relaxation time, as T 21 (0.01-10 ms), T 22 (10-150 ms), and T 23 (150-1000 ms) were ascribed to cell wall protons, cytoplasmic water, and vacuolar water respectively. Contents of cytoplasmic (p < 0.05) and vacuolar water (p < 0.01) changed significantly with storage time. Magnetic resonance imaging results illustrated that water distributes uniformly in fresh tissue. With prolonged storage time, free water content increased gradually, and then internal damage occurred. Sweet cherry softening closely correlated with moisture loss during low-temperature storage. LF-NMR is a useful technique to investigate moisture migration of fruits and vegetables. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

  16. Cold Helium Pressurization for Liquid Oxygen/Liquid Methane Propulsion Systems: Fully-Integrated Hot-Fire Test Results

    NASA Technical Reports Server (NTRS)

    Morehead, R. L.; Atwell, M. J.; Melcher, J. C.; Hurlbert, E. A.

    2016-01-01

    Hot-fire test demonstrations were successfully conducted using a cold helium pressurization system fully integrated into a liquid oxygen (LOX) / liquid methane (LCH4) propulsion system (Figure 1). Cold helium pressurant storage at near liquid nitrogen (LN2) temperatures (-275 F and colder) and used as a heated tank pressurant provides a substantial density advantage compared to ambient temperature storage. The increased storage density reduces helium pressurant tank size and mass, creating payload increases of 35% for small lunar-lander sized applications. This degree of mass reduction also enables pressure-fed propulsion systems for human-rated Mars ascent vehicle designs. Hot-fire test results from the highly-instrumented test bed will be used to demonstrate system performance and validate integrated models of the helium and propulsion systems. A pressurization performance metric will also be developed as a means to compare different active pressurization schemes.

  17. Effects of Storage Temperature and Extraction Procedure on Recovery of Plant-parasitic Nematodes from Field Soils

    PubMed Central

    Barker, K. R.; Nusbaum, C. J.; Nelson, L. A.

    1969-01-01

    Storage of nematodes in soil at -15 C for 1 to 16 weeks greatly increased nematode recovery by a sugar-flotation-sieving procedure. One week of exposure to -15 C killed all nematodes except Pratylenchus zeae and Tylenchorhynchus claytoni which were recoverable in decreasing numbers up to 10 weeks by the Baermann funnel method. Optimum storage temperature for survival of most nematode species was 13 C. The numbers of Meloidogyne incognita, T. claytoni, Belonolaimus Iongicaudatus, and P. zeae recoverable by either extraction method remained constant or increased when stored at 13-24 C for 16 weeks. This was also true for Helicotylenchtts dihystera and Xiphinema americanum extracted by the Baermann funnel technique, whereas the numbers retrieved by the sugar-flotation-sieving method decreased slightly. All species except T. claytoni decreased appreciably in soil stored at 36 C. PMID:19325684

  18. Physicochemical changes of 'Phulae' pineapple fruit treated with short-term anoxia during ambient storage.

    PubMed

    Techavuthiporn, Chairat; Boonyaritthongchai, Panida; Supabvanich, Suriyan

    2017-08-01

    The effects of short-term anoxia exposure for 16h on physicochemical changes of 'Phulae' pineapple fruit stored at ambient temperature (25±2°C) were investigated. The respiratory rate of the fruit was induced by the anoxia treatment. However, it retarded the increase in moisture loss and maintained both flesh and pulp colour by inhibiting polyphenol oxidase (PPO) activity of the both tissues. The anoxia exposure delayed the increase in total sugar content and enhanced total ascorbic acid content during storage. The half-cut pineapple fruit showed that the anoxia exposure completely inhibited internal transparency of the flesh tissue adjacent to core during the storage. In conclusion, the short-term anoxia exposure for 16h maintained postharvest quality, retarded physiological disorder and enhanced nutritional values of the pineapple fruit stored at ambient temperature (25±2°C). Copyright © 2017 Elsevier Ltd. All rights reserved.

  19. Changes in the microbiological quality of mangrove oysters (Crassostrea brasiliana) during different storage conditions.

    PubMed

    Montanhini, Maike Taís Maziero; Montanhini Neto, Roberto

    2015-01-01

    This study aimed to determine the effect of temperature and period of postharvest storage on the microbiological quality and shelf life of raw mangrove oysters, Crassostrea brasiliana. A total of 150 dozen oysters were collected directly from the points of extraction or cultivation in southern Brazil, and in the laboratory, they were stored raw at 5, 10, 15, 20, and 25°C for 1, 4, 8, 11, and 15 days. On each of these days, the oysters were subjected to microbiological analyses of aerobic mesophilic count, total coliforms, enterococci, Escherichia coli, Staphylococcus aureus, and Salmonella. None of the tested samples under any storage condition showed contamination levels above those allowed by Brazilian legislation for E. coli, S. aureus, and Salmonella, and there was no change (P > 0.05) in the counts of these microorganisms due to the temperature and/or period of oyster storage. Counts of enterococci and total coliforms showed a tendency to increase (P < 0.05) among the different temperatures tested. Raw mangrove oysters remain in safe microbiological conditions for consumption up to 8 days after harvesting, regardless of temperature, and their shelf life may be extended to 15 days if they are stored at temperatures not exceeding 15°C.

  20. FPGA-based prototype storage system with phase change memory

    NASA Astrophysics Data System (ADS)

    Li, Gezi; Chen, Xiaogang; Chen, Bomy; Li, Shunfen; Zhou, Mi; Han, Wenbing; Song, Zhitang

    2016-10-01

    With the ever-increasing amount of data being stored via social media, mobile telephony base stations, and network devices etc. the database systems face severe bandwidth bottlenecks when moving vast amounts of data from storage to the processing nodes. At the same time, Storage Class Memory (SCM) technologies such as Phase Change Memory (PCM) with unique features like fast read access, high density, non-volatility, byte-addressability, positive response to increasing temperature, superior scalability, and zero standby leakage have changed the landscape of modern computing and storage systems. In such a scenario, we present a storage system called FLEET which can off-load partial or whole SQL queries to the storage engine from CPU. FLEET uses an FPGA rather than conventional CPUs to implement the off-load engine due to its highly parallel nature. We have implemented an initial prototype of FLEET with PCM-based storage. The results demonstrate that significant performance and CPU utilization gains can be achieved by pushing selected query processing components inside in PCM-based storage.

  1. Three-dimensional analysis for liquid hydrogen in a cryogenic storage tank with heat pipe pump system

    NASA Astrophysics Data System (ADS)

    Ho, Son H.; Rahman, Muhammad M.

    2008-01-01

    This paper presents a study on fluid flow and heat transfer of liquid hydrogen in a zero boil-off cryogenic storage tank in a microgravity environment. The storage tank is equipped with an active cooling system consisting of a heat pipe and a pump-nozzle unit. The pump collects cryogen at its inlet and discharges it through its nozzle onto the evaporator section of the heat pipe in order to prevent the cryogen from boiling off due to the heat leaking through the tank wall from the surroundings. A three-dimensional (3-D) finite element model is employed in a set of numerical simulations to solve for velocity and temperature fields of liquid hydrogen in steady state. Complex structures of 3-D velocity and temperature distributions determined from the model are presented. Simulations with an axisymmetric model were also performed for comparison. Parametric study results from both models predict that as the speed of the cryogenic fluid discharged from the nozzle increases, the mean or bulk cryogenic fluid speed increases linearly and the maximum temperature within the cryogenic fluid decreases.

  2. Thermal performance of phase change wallboard for residential cooling application

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Feustel, H.E.; Stetiu, C.

    1997-04-01

    Cooling of residential California buildings contributes significantly to electrical consumption and peak power demand mainly due to very poor load factors in milder climates. Thermal mass can be utilized to reduce the peak-power demand, downsize the cooling systems, and/or switch to low-energy cooling sources. Large thermal storage devices have been used in the past to overcome the shortcomings of alternative cooling sources, or to avoid high demand charges. The manufacturing of phase change material (PCM) implemented in gypsum board, plaster or other wall-covering material, would permit the thermal storage to become part of the building structure. PCMs have two importantmore » advantages as storage media: they can offer an order-of-magnitude increase in thermal storage capacity, and their discharge is almost isothermal. This allows the storage of high amounts of energy without significantly changing the temperature of the room envelope. As heat storage takes place inside the building, where the loads occur, rather than externally, additional transport energy is not required. RADCOOL, a thermal building simulation program based on the finite difference approach, was used to numerically evaluate the latent storage performance of treated wallboard. Extended storage capacity obtained by using double PCM-wallboard is able to keep the room temperatures close to the upper comfort limits without using mechanical cooling. Simulation results for a living room with high internal loads and weather data for Sunnyvale, California, show significant reduction of room air temperature when heat can be stored in PCM-treated wallboards.« less

  3. Numerical analysis of single tank thermocline thermal storage system for concentrated solar power plant

    NASA Astrophysics Data System (ADS)

    Afrin, Samia

    The overall efficiency of a Concentrating Solar Power (CSP) plant depends on the effectiveness of Thermal Energy Storage (TES) system. A Single tank TES system has potential to provide effective solution. In a single tank TES system, a thermocline region, which produces the temperature gradient between hot and cold storage fluid by density difference, is used. Preservation of this thermocline region in the tank during charging and discharging cycles depends on the uniformity of the velocity profile at any horizontal plane. One of the major challenges for the single tank thermocline is actually maintaining the thermocline region in the tank, so that it does not spread out to occupy the entire tank. Since the thermocline is a horizontal surface, the hot and cold fluid must be introduce in such a way that it does not disturb the thermocline. If the fluid is introduced in a jet stream, it will disturb the thermocline and mix the hot and cold fluids into a homogeneous medium. So the objective of this thesis is to preserve the thermocline region by maximizing the uniformity of the velocity distribution. An ideal distributor will minimize the thermocline spreading and hence maximize the useable form of thermal energy storage in a single tank system. The performance of two different types of distributors: pipe flow distributor and honeycomb distributor, were checked. The effectiveness of the pipe flow distributor was checked by varying the dimension of the geometry i.e. number of holes, distance between the holes, position of the holes and number of distributor pipes. Thermal energy storage system from solar power relies on high temperature thermal storage units for continuous operation. The storage units should have facilitated with high thermal conductivity and heat capacity storage fluid. Hence it is necessary to find a better performing heat transfer fluid at higher operating temperature. Novel materials such as nanomaterial additives can become cost effective and can increase the operating range of the storage facilities to higher range of temperatures. In this work HitecRTM molten salt is considered as the heat transfer fluid (HTF). The operating temperature of this HTF is 300-500°C. So to increase the thermal properties of this HTF nanomaterial has been added. The effective thermal conductivity and specific heat capacity of the nanofluid were calculated and the thermal effect of this nanofluid was observed from the simulation result.

  4. Effect of ripeness stage during processing on Listeria monocytogenes growth on fresh-cut 'Conference' pears.

    PubMed

    Colás-Medà, Pilar; Abadias, Maribel; Alegre, Isabel; Usall, Josep; Viñas, Inmaculada

    2015-08-01

    There are several factors that affect the shelf life of fresh-cut fruit, including the cultivar, the ripeness stage of the fruit during processing and the fruit's storage atmosphere and temperature. The effect of fruit ripeness during processing on the survival and growth of Listeria monocytogenes on fresh-cut 'Conference' pear slices at different temperatures (5, 10 and 20 °C) was studied. The four ripeness stages studied in this work (assessed by a fruit's firmness) were mature-green (54-60 N), partially ripe (43-53 N), ripe (31-42 N) and overripe (<31 N). In our studies, pH, acidity and soluble solids content did not significantly change during conditioning at 20 °C. L. monocytogenes grew under all experimental conditions, showing an increase of approximately 2 log CFU g(-1) after 8 days of storage at 5 °C. There were significant differences in the L. monocytogenes population between different ripeness stages at the end of the experiments at 10 and 20 °C. Regardless of the ripeness stage of a fresh-cut pear, the growth potential of L. monocytogenes increased with increasing temperature. A pear's ripeness stage during processing is an important consideration to ensure the quality of a fresh-cut pear, but it is not as important for preventing L. monocytogenes growth at common storage temperatures. Copyright © 2015 Elsevier Ltd. All rights reserved.

  5. Headspace oxygen as a hurdle to improve the safety of in-pack pasteurized chilled food during storage at different temperatures.

    PubMed

    Muñoz, Nydia; Bhunia, Kanishka; Zhang, Hongchao; Barbosa-Cánovas, Gustavo V; Tang, Juming; Sablani, Shyam

    2017-07-17

    This study investigated the use of headspace oxygen in a model food system to prevent the growth of anaerobic pathogenic bacteria in in-pack pasteurized food at various storage temperatures. Three model food formulations prepared with tryptic soy broth and three agar concentrations (0.1, 0.4 and 1.0%) were sealed without removing the air from the package in high oxygen barrier pouches (OTR=0.3cm 3 /m 2 ·day·atm). Important properties influencing bacterial growth, including pH and water activity (a w ) were determined. The oxygen sorption kinetics of each model food were obtained at three different storage temperatures (8, 12, and 20°C) using an OxySense Gen III 300 system. An analytical solution of Fick's second law was used to determine the O 2 diffusion coefficient. Growth challenge studies at 12 and 20°C were conducted at three selected locations (top, center and bottom layers) in model foods containing 1% agar. Model foods were inoculated with Clostridium sporogenes PA 3679 (300spores/mL), and were classified as low-acid (pH>4.5, a w >0.85). When the storage temperature decreased from 20 to 8°C, the oxygen diffusion decreased from 0.82×10 -9 m 2 /s to 0.68×10 -9 m 2 /s. As the agar concentration was increased from 0.1 to 1.0%, the effective oxygen permeability decreased significantly (p=0.007) from 0.88×10 -9 m 2 /s to 0.65×10 -9 m 2 /s. When the inoculated model foods were stored at 12°C for 14days, C. sporogenes PA 3679 was unable to grow. As the storage temperature was increased to 20°C, significant bacterial growth was observed with storage time (p<0.0001), and the C. sporogenes PA 3679 population increased by around 6logCFU/g. However, the location of the food did not influence the growth distribution of C. sporogenes PA 3679. These results demonstrate that oxygen diffusion from the pouch headspace was primarily limited to the food surface. Findings suggest that the air/oxygen present in the package headspace may not be considered as a food safety hurdle in the production of pasteurized packaged food. Copyright © 2017 Elsevier B.V. All rights reserved.

  6. A Comparison Study: The New Extended Shelf Life Isopropyl Ester PMR Technology versus The Traditional Methyl Ester PMR Approach

    NASA Technical Reports Server (NTRS)

    Alston, William B.; Scheiman, Daniel A.; Sivko, Gloria S.

    2005-01-01

    Polymerization of Monomeric Reactants (PMR) monomer solutions and carbon cloth prepregs of PMR II-50 and VCAP-75 were prepared using both the traditional limited shelf life methanol based PMR approach and a novel extended shelf life isopropanol based PMR approach. The methyl ester and isopropyl ester based PMR monomer solutions and PMR prepregs were aged for up to four years at freezer and room temperatures. The aging products formed were monitored using high pressure liquid chromatography (HPLC). The composite processing flow characteristics and volatile contents of the aged prepregs were also correlated versus room temperature storage time. Composite processing cycles were developed and six ply cloth laminates were fabricated with prepregs after various extended room temperature storage times. The composites were then evaluated for glass transition temperature (Tg), thermal decomposition temperature (Td), initial flexural strength (FS) and modulus (FM), long term (1000 hours at 316 C) thermal oxidative stability (TOS), and retention of FS and FM after 1000 hours aging at 316 C. The results for each ester system were comparable. Freezer storage was found to prevent the formation of aging products for both ester systems. Room temperature storage of the novel isopropyl ester system increased PMR monomer solution and PMR prepreg shelf life by at least an order of magnitude while maintaining composite properties.

  7. Behavioral pattern of physicochemical constituents of the postharvest mango (Mangifera indica L.) influenced by storage stimuli.

    PubMed

    Islam, Md Khairul

    2013-12-15

    An investigation was carried at the laboratory of the Department of Biochemistry and Molecular Biology, University of Rajshahi, Bangladesh during the period from May, 2010 to September, 2011 to study the behavioral pattern of some physicochemical constituents of the mango pulp. The experiment was comprised of two popular mango cultivars in Bangladesh (viz., Langra and Khirshapat) and six storage stimuli, namely control, paraffin coating, perforated polyethylene cover, unperforated polyethylene cover, hot water (55 +/- 1 degree C) and low temperature (4 +/- 1 degree C). The two factors experiment was assigned in randomized complete block design with tree replicates. The varieties had profound variation in terms of most of the characters studied in the laboratory condition. Initially the Langra significantly enriched a greater amount of vitamin C (151.23 mg/100 g) and titratable acidity (4.31%) and these were decreased gradually with the progress of storage period. The Khirshapat showed higher pulp pH (5.83); produced enormous amount of TSS (18.00%) and sugar (TS = 17.62%, RS = 6.51% and NRS = 11.06%) content at 12th day of storage. The pH, TSS, sugar (TS, RS and NRS) content of mango pulp was rapidly increased, whereas vitamin C and titratable acidity decreased drastically from the untreated mangoes. On the other hand, low temperature retarded the changes. The Langra using low temperature (4 +/- 1 degree C) exhibited lower diminishing tendency in vitamin C and titratable acidity and also using no treatment slightly increased TSS; enriched enormous amount of sugar (TS, RS and NRS). Therefore, low temperature (4 +/- 1 degree C) was found satisfactory for delay ripening and postharvest changes of mango in storage condition.

  8. Effects of storage conditions of Moringa oleifera seeds on its performance in coagulation.

    PubMed

    Katayon, S; Noor, M J Megat Mohd; Asma, M; Ghani, L A Abdul; Thamer, A M; Azni, I; Ahmad, J; Khor, B C; Suleyman, A M

    2006-09-01

    Moringa oleifera is a plant whose seeds have coagulation properties for treating water and wastewater. In this study the coagulation efficiency of Moringa oleifera kept in different storage conditions were studied. The Moringa oleifera seeds were stored at different conditions and durations; open container and closed container at room temperature (28 degrees C) and refrigerator (3 degrees C) for durations of 1, 3 and 5 months. Comparison between turbidity removal efficiency of Moringa oleifera kept in refrigerator and room temperature revealed that there was no significant difference between them. The Moringa oleifera kept in refrigerator and room temperature for one month showed higher turbidity removal efficiency, compared to those kept for 3 and 5 months, at both containers. The coagulation efficiency of Moringa oleifera was found to be dependent on initial turbidity of water samples. Highest turbidity removals were obtained for water with very high initial turbidity. In summary coagulation efficiency of Moringa oleifera was found independent of storage temperature and container, however coagulation efficiency of Moringa oleifera decreased as storage duration increased. In addition, Moringa oleifera can be used as a potential coagulant especially for very high turbidity water.

  9. The impact of a cold chain break on the survival of Salmonella enterica and Listeria monocytogenes on minimally processed 'Conference' pears during their shelf life.

    PubMed

    Colás-Medà, Pilar; Viñas, Inmaculada; Alegre, Isabel; Abadias, Maribel

    2017-07-01

    In recent years, improved detection methods and increased fresh-cut processing of produce have led to an increased number of outbreaks associated with fresh fruits and vegetables. During fruit and vegetable processing, natural protective barriers are removed and tissues are cut, causing nutrient rich exudates and providing attachment sites for microbes. Consequently, fresh-cut produce is more susceptible to microbial proliferation than whole produce. The aim of this study was to examine the impact of storage temperature on the growth and survival of Listeria monocytogenes and Salmonella enterica on a fresh-cut 'Conference' pear over an 8 day storage period. Pears were cut, dipped in antioxidant solution, artificially inoculated with L. monocytogenes and S. enterica, packed under modified atmospheric conditions simulating commercial applications and stored in properly refrigerated conditions (constant storage at 4 °C for 8 days) or in temperature abuse conditions (3 days at 4 °C plus 5 days at 8 °C). After 8 days of storage, both conditions resulted in a significant decrease of S. enterica populations on pear wedges. In contrast, when samples were stored at 4 °C for 8 days, L. monocytogenes populations increased 1.6 logarithmic units, whereas under the temperature abuse conditions, L. monocytogenes populations increased 2.2 logarithmic units. Listeria monocytogenes was able to grow on fresh-cut pears processed under the conditions described here, despite low pH, refrigeration and use of modified atmosphere. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry.

  10. Long-term soil warming and Carbon Cycle Feedbacks to the Climate System

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Melillo, Jerry M.

    2014-04-30

    The primary objective of the proposed research was to quantify and explain the effects of a sustained in situ 5oC soil temperature increase on net carbon (C) storage in a northeastern deciduous forest ecosystem. The research was done at an established soil warming experiment at the Harvard Forest in central Massachusetts – Barre Woods site established in 2001. In the field, a series of plant and soil measurements were made to quantify changes in C storage in the ecosystem and to provide insights into the possible relationships between C-storage changes and nitrogen (N) cycling changes in the warmed plots. Fieldmore » measurements included: 1) annual woody increment; 2) litterfall; 3) carbon dioxide (CO2) efflux from the soil surface; 4) root biomass and respiration; 5) microbial biomass; and 6) net N mineralization and net nitrification rates. This research was designed to increase our understanding of how global warming will affect the capacity of temperate forest ecosystems to store C. The work explored how soil warming changes the interactions between the C and N cycles, and how these changes affect land-atmosphere feedbacks. This core research question framed the project – What are the effects of a sustained in situ 5oC soil temperature increase on net carbon (C) storage in a northeastern deciduous forest ecosystem? A second critical question was addressed in this research – What are the effects of a sustained in situ 5{degrees}C soil temperature increase on nitrogen (N) cycling in a northeastern deciduous forest ecosystem?« less

  11. Effect of modified atmosphere packaging on Quality Index Method (QIM) scores of farmed gilthead seabream (Sparus aurata L.) at low and abused temperatures.

    PubMed

    Campus, Marco; Bonaglini, Elia; Cappuccinelli, Roberto; Porcu, Maria Cristina; Tonelli, Roberto; Roggio, Tonina

    2011-04-01

    A Quality Index Method (QIM) scheme was developed for modified atmosphere packaging (MAP) packed gilthead seabream, and the effect of MAP gas mixtures (60% CO2 and 40% N2; 60% CO2, 30% O2, and 10% N2), temperature (2, 4, and 8 °C), and time of storage on QI scores was assessed. QI scores were crossed with sensory evaluation of cooked fish according to a modified Torry scheme to establish the rejection point. In order to reduce redundant parameters, a principal component analysis was applied on preliminary QIM parameters scores coming from the best performing MAP among those tested. The final QIM scheme consists of 13 parameters and a maximum demerit score of 25. The maximum storage time was found to be 13 d at 4 °C for MAP 60% CO2 and 40% N2. Storage at 2 °C do not substantially improved sensory parameters scores, while storage under temperature abuse (8 °C) accelerated drastically the rate of increase of QI scores and reduced the maximum storage time to 6 d.

  12. Different storage conditions influence biocompatibility and physicochemical properties of iron oxide nanoparticles.

    PubMed

    Zaloga, Jan; Janko, Christina; Agarwal, Rohit; Nowak, Johannes; Müller, Robert; Boccaccini, Aldo R; Lee, Geoffrey; Odenbach, Stefan; Lyer, Stefan; Alexiou, Christoph

    2015-04-24

    Superparamagnetic iron oxide nanoparticles (SPIONs) have attracted increasing attention in many biomedical fields. In magnetic drug targeting SPIONs are injected into a tumour supplying artery and accumulated inside the tumour with a magnet. The effectiveness of this therapy is thus dependent on magnetic properties, stability and biocompatibility of the particles. A good knowledge of the effect of storage conditions on those parameters is of utmost importance for the translation of the therapy concept into the clinic and for reproducibility in preclinical studies. Here, core shell SPIONs with a hybrid coating consisting of lauric acid and albumin were stored at different temperatures from 4 to 45 °C over twelve weeks and periodically tested for their physicochemical properties over time. Surprisingly, even at the highest storage temperature we did not observe denaturation of the protein or colloidal instability. However, the saturation magnetisation decreased by maximally 28.8% with clear correlation to time and storage temperature. Furthermore, the biocompatibility was clearly affected, as cellular uptake of the SPIONs into human T-lymphoma cells was crucially dependent on the storage conditions. Taken together, the results show that the particle properties undergo significant changes over time depending on the way they are stored.

  13. Malaria rapid diagnostic test transport and storage conditions in Burkina Faso, Senegal, Ethiopia and the Philippines

    PubMed Central

    2012-01-01

    Background As more point of care diagnostics become available, the need to transport and store perishable medical commodities to remote locations increases. As with other diagnostics, malaria rapid diagnostic tests (RDTs) must be highly reliable at point of use, but exposure to adverse environmental conditions during distribution has the potential to degrade tests and accuracy. In remote locations, poor quality diagnostics and drugs may have significant negative health impact that is not readily detectable by routine monitoring. This study assessed temperature and humidity throughout supply chains used to transport and store health commodities, such as RDTs. Methods Monitoring devices capable of recording temperature and humidity were deployed to Burkina Faso (8), Senegal (10), Ethiopia (13) and the Philippines (6) over a 13-month period. The devices travelled through government supply chains, usually alongside RDTs, to health facilities where RDTs are stored, distributed and used. The recording period spanned just over a year, in order to avoid any biases related to seasonal temperature variations. Results In the four countries, storage and transport temperatures regularly exceeded 30.0°C; maximum humidity level recorded was above 94% for the four countries. In three of the four countries, temperatures recorded at central storage facilities exceeded pharmaceutical storage standards for over 20% of the time, in another case for a majority of the time; and sometimes exceeded storage temperatures at peripheral sites. Conclusions Malaria RDTs were regularly exposed to temperatures above recommended limits for many commercially-available RDTs and other medical commodities such as drugs, but rarely exceeded the recommended storage limits for particular products in use in these countries. The results underline the need to select RDTs, and other commodities, according to expected field conditions, actively manage the environmental conditions in supply chains in tropical and sub-tropical climates. This would benefit from a re-visit of current global standards on stability of medical commodities based in tropical and sub-tropical climatic zones. PMID:23217104

  14. Malaria rapid diagnostic test transport and storage conditions in Burkina Faso, Senegal, Ethiopia and the Philippines.

    PubMed

    Albertini, Audrey; Lee, Evan; Coulibaly, Sheick Oumar; Sleshi, Markos; Faye, Babacar; Mationg, Mary Lorraine; Ouedraogo, Kadi; Tsadik, Abeba G; Feleke, Sendeaw Maksha; Diallo, Ibrahima; Gaye, Oumar; Luchavez, Jennifer; Bennett, Jessica; Bell, David

    2012-12-06

    As more point of care diagnostics become available, the need to transport and store perishable medical commodities to remote locations increases. As with other diagnostics, malaria rapid diagnostic tests (RDTs) must be highly reliable at point of use, but exposure to adverse environmental conditions during distribution has the potential to degrade tests and accuracy. In remote locations, poor quality diagnostics and drugs may have significant negative health impact that is not readily detectable by routine monitoring. This study assessed temperature and humidity throughout supply chains used to transport and store health commodities, such as RDTs. Monitoring devices capable of recording temperature and humidity were deployed to Burkina Faso (8), Senegal (10), Ethiopia (13) and the Philippines (6) over a 13-month period. The devices travelled through government supply chains, usually alongside RDTs, to health facilities where RDTs are stored, distributed and used. The recording period spanned just over a year, in order to avoid any biases related to seasonal temperature variations. In the four countries, storage and transport temperatures regularly exceeded 30.0°C; maximum humidity level recorded was above 94% for the four countries. In three of the four countries, temperatures recorded at central storage facilities exceeded pharmaceutical storage standards for over 20% of the time, in another case for a majority of the time; and sometimes exceeded storage temperatures at peripheral sites. Malaria RDTs were regularly exposed to temperatures above recommended limits for many commercially-available RDTs and other medical commodities such as drugs, but rarely exceeded the recommended storage limits for particular products in use in these countries. The results underline the need to select RDTs, and other commodities, according to expected field conditions, actively manage the environmental conditions in supply chains in tropical and sub-tropical climates. This would benefit from a re-visit of current global standards on stability of medical commodities based in tropical and sub-tropical climatic zones.

  15. Multi-trait analysis of post-harvest storage in rocket salad (Diplotaxis tenuifolia) links sensorial, volatile and nutritional data.

    PubMed

    Spadafora, Natasha D; Amaro, Ana L; Pereira, Maria J; Müller, Carsten T; Pintado, Manuela; Rogers, Hilary J

    2016-11-15

    Rocket salad (Diplotaxis tenuifolia; wild rocket) is an important component of ready to eat salads providing a distinct peppery flavour and containing nutritionally relevant compounds. Quality deteriorates during post-harvest, in relation to time and storage temperature amongst other factors. Volatile organic compounds (VOCs) are easily measurable from rocket leaves and may provide useful quality indicators for e.g. changes in isothiocyanates derived from nutritionally important glucosinolates. VOC profiles discriminated storage temperatures (0, 5 and 10°C) and times (over 14days). More specifically, concentrations of aldehydes and isothiocyanates decreased with time paralleling a fall in vitamin C and a reduction in sensorial quality at the two higher temperatures. Sulphur containing compounds rise at later time-points and at higher temperatures coincident with an increase in microbial titre, mirroring a further drop in sensorial quality thus indicating their contribution to off-odours. Copyright © 2016 The Authors. Published by Elsevier Ltd.. All rights reserved.

  16. Furan formation during storage and reheating of sterilised vegetable purées.

    PubMed

    Palmers, Stijn; Grauwet, Tara; Buvé, Carolien; Van de Vondel, Lore; Kebede, Biniam T; Hendrickx, Marc E; Van Loey, Ann

    2015-01-01

    To this day, research for furan mitigation has mostly targeted the levels of food production and handling of prepared foods by the consumer. However, part of the furan concentrations found in commercially available food products might originate from chemical deterioration reactions during storage. A range of individual vegetable purées was stored at two different temperatures to investigate the effects of storage on the furan concentrations of shelf-stable, vegetable-based foods. After 5 months of storage at 35°C (temperature-abuse conditions), a general increase in furan concentrations was observed. The furan formation during storage could be reduced by storing the vegetable purées at a refrigerated temperature of 4°C, at which the furan concentrations remained approximately constant for at least 5 months. Following storage, the vegetable purées were briefly reheated to 90°C to simulate the effect of the final preparation step before consumption. Contrary to storage, furan concentrations decreased as a result of evaporative losses. Both refrigerated storage and the reheating step prior to consumption showed the potential of mitigation measures for furan formation in vegetable-based foods (e.g. canned vegetables, ready-to-eat soups, sauces or baby foods). Next to furan, the vegetable purées were analysed for 2- and 3-methylfuran. Tomato was very susceptible to the formation of both alkylated derivatives of furan, as opposed to the other vegetables in this study. Methylfuran concentrations rapidly decreased during storage, which was contrary to the results observed for furan.

  17. Effects of storage temperature on airway exosome integrity for diagnostic and functional analyses

    PubMed Central

    Maroto, Rosario; Zhao, Yingxin; Jamaluddin, Mohammad; Popov, Vsevolod L.; Wang, Hongwang; Kalubowilage, Madumali; Zhang, Yueqing; Luisi, Jonathan; Sun, Hong; Culbertson, Christopher T.; Bossmann, Stefan H.; Motamedi, Massoud; Brasier, Allan R.

    2017-01-01

    ABSTRACT Background: Extracellular vesicles contain biological molecules specified by cell-type of origin and modified by microenvironmental changes. To conduct reproducible studies on exosome content and function, storage conditions need to have minimal impact on airway exosome integrity. Aim: We compared surface properties and protein content of airway exosomes that had been freshly isolated vs. those that had been treated with cold storage or freezing. Methods: Mouse bronchoalveolar lavage fluid (BALF) exosomes purified by differential ultracentrifugation were analysed immediately or stored at +4°C or −80°C. Exosomal structure was assessed by dynamic light scattering (DLS), transmission electron microscopy (TEM) and charge density (zeta potential, ζ). Exosomal protein content, including leaking/dissociating proteins, were identified by label-free LC-MS/MS. Results: Freshly isolated BALF exosomes exhibited a mean diameter of 95 nm and characteristic morphology. Storage had significant impact on BALF exosome size and content. Compared to fresh, exosomes stored at +4°C had a 10% increase in diameter, redistribution to polydisperse aggregates and reduced ζ. Storage at −80°C produced an even greater effect, resulting in a 25% increase in diameter, significantly reducing the ζ, resulting in multilamellar structure formation. In fresh exosomes, we identified 1140 high-confidence proteins enriched in 19 genome ontology biological processes. After storage at room temperature, 848 proteins were identified. In preparations stored at +4°C, 224 proteins appeared in the supernatant fraction compared to the wash fractions from freshly prepared exosomes; these proteins represent exosome leakage or dissociation of loosely bound “peri-exosomal” proteins. In preparations stored at −80°C, 194 proteins appeared in the supernatant fraction, suggesting that distinct protein groups leak from exosomes at different storage temperatures. Conclusions: Storage destabilizes the surface characteristics, morphological features and protein content of BALF exosomes. For preservation of the exosome protein content and representative functional analysis, airway exosomes should be analysed immediately after isolation. PMID:28819550

  18. Effects of storage temperature on airway exosome integrity for diagnostic and functional analyses.

    PubMed

    Maroto, Rosario; Zhao, Yingxin; Jamaluddin, Mohammad; Popov, Vsevolod L; Wang, Hongwang; Kalubowilage, Madumali; Zhang, Yueqing; Luisi, Jonathan; Sun, Hong; Culbertson, Christopher T; Bossmann, Stefan H; Motamedi, Massoud; Brasier, Allan R

    2017-01-01

    Background : Extracellular vesicles contain biological molecules specified by cell-type of origin and modified by microenvironmental changes. To conduct reproducible studies on exosome content and function, storage conditions need to have minimal impact on airway exosome integrity. Aim : We compared surface properties and protein content of airway exosomes that had been freshly isolated vs. those that had been treated with cold storage or freezing. Methods : Mouse bronchoalveolar lavage fluid (BALF) exosomes purified by differential ultracentrifugation were analysed immediately or stored at +4°C or -80°C. Exosomal structure was assessed by dynamic light scattering (DLS), transmission electron microscopy (TEM) and charge density (zeta potential, ζ). Exosomal protein content, including leaking/dissociating proteins, were identified by label-free LC-MS/MS. Results : Freshly isolated BALF exosomes exhibited a mean diameter of 95 nm and characteristic morphology. Storage had significant impact on BALF exosome size and content. Compared to fresh, exosomes stored at +4°C had a 10% increase in diameter, redistribution to polydisperse aggregates and reduced ζ. Storage at -80°C produced an even greater effect, resulting in a 25% increase in diameter, significantly reducing the ζ, resulting in multilamellar structure formation. In fresh exosomes, we identified 1140 high-confidence proteins enriched in 19 genome ontology biological processes. After storage at room temperature, 848 proteins were identified. In preparations stored at +4°C, 224 proteins appeared in the supernatant fraction compared to the wash fractions from freshly prepared exosomes; these proteins represent exosome leakage or dissociation of loosely bound "peri-exosomal" proteins. In preparations stored at -80°C, 194 proteins appeared in the supernatant fraction, suggesting that distinct protein groups leak from exosomes at different storage temperatures. Conclusions : Storage destabilizes the surface characteristics, morphological features and protein content of BALF exosomes. For preservation of the exosome protein content and representative functional analysis, airway exosomes should be analysed immediately after isolation.

  19. Ecosystem carbon storage does not vary with mean annual temperature in Hawaiian tropical montane wet forests.

    PubMed

    Selmants, Paul C; Litton, Creighton M; Giardina, Christian P; Asner, Gregory P

    2014-09-01

    Theory and experiment agree that climate warming will increase carbon fluxes between terrestrial ecosystems and the atmosphere. The effect of this increased exchange on terrestrial carbon storage is less predictable, with important implications for potential feedbacks to the climate system. We quantified how increased mean annual temperature (MAT) affects ecosystem carbon storage in above- and belowground live biomass and detritus across a well-constrained 5.2 °C MAT gradient in tropical montane wet forests on the Island of Hawaii. This gradient does not systematically vary in biotic or abiotic factors other than MAT (i.e. dominant vegetation, substrate type and age, soil water balance, and disturbance history), allowing us to isolate the impact of MAT on ecosystem carbon storage. Live biomass carbon did not vary predictably as a function of MAT, while detrital carbon declined by ~14 Mg of carbon ha(-1) for each 1 °C rise in temperature - a trend driven entirely by coarse woody debris and litter. The largest detrital pool, soil organic carbon, was the most stable with MAT and averaged 48% of total ecosystem carbon across the MAT gradient. Total ecosystem carbon did not vary significantly with MAT, and the distribution of ecosystem carbon between live biomass and detritus remained relatively constant across the MAT gradient at ~44% and ~56%, respectively. These findings suggest that in the absence of alterations to precipitation or disturbance regimes, the size and distribution of carbon pools in tropical montane wet forests will be less sensitive to rising MAT than predicted by ecosystem models. This article also provides needed detail on how individual carbon pools and ecosystem-level carbon storage will respond to future warming. © 2014 John Wiley & Sons Ltd.

  20. Biogenic amine formation and nitrite reactions in meat batter as affected by high-pressure processing and chilled storage.

    PubMed

    Ruiz-Capillas, C; Aller-Guiote, P; Carballo, J; Colmenero, F Jiménez

    2006-12-27

    Changes in biogenic amine formation and nitrite depletion in meat batters as affected by pressure-temperature combinations (300 MPa/30 min/7, 20, and 40 degrees C), cooking process (70 degrees C/30 min), and storage (54 days/2 degrees C) were studied. Changes in residual nitrite concentration in raw meat batters were conditioned by the temperature and not by the pressure applied. Cooking process decreased (P < 0.05) the residual nitrite concentration in all samples. High-pressure processing and cooking treatment increased (P < 0.05) the nitrate content. Whereas protein-bound nitrite concentration decreased with pressure processing, no effect was observed with the heating process of meat batters. High-pressure processing conditions had no effect on the rate of residual nitrite loss throughout the storage. The application of high pressure decreased (P < 0.05) the concentration of some biogenic amines (tyramine, agmatine, and spermine). Irrespective of the high processing conditions, generally, throughout storage biogenic amine levels did not change or increased, although quantitatively this effect was not very important.

  1. Warm water temperatures and shifts in seasonality increase trout recruitment but only moderately decrease adult size in western North American tailwaters

    USGS Publications Warehouse

    Dibble, Kimberly L.; Yackulic, Charles B.; Kennedy, Theodore A.

    2018-01-01

    Dams throughout western North America have altered thermal regimes in rivers, creating cold, clear “tailwaters” in which trout populations thrive. Ongoing drought in the region has led to highly publicized reductions in reservoir storage and raised concerns about potential reductions in downstream flows. Large changes in riverine thermal regimes may also occur as reservoir water levels drop, yet this potential impact has received far less attention. We analyzed historic water temperature and fish population data to anticipate how trout may respond to future changes in the magnitude and seasonality of river temperatures. We found that summer temperatures were inversely related to reservoir water level, with warm temperatures associated with reduced storage and with dams operated as run-of-river units. Variation in rainbow trout (Oncorhynchus mykiss) recruitment was linked to water temperature variation, with a 5-fold increase in recruitment occurring at peak summer temperatures (18 °C vs. 7 °C) and a 2.5-fold increase in recruitment when peak temperatures occurred in summer rather than fall. Conversely, adult trout size was only moderately related to temperature. Rainbow and brown trout (Salmo trutta) size decreased by ~24 mm and 20 mm, respectively, as mean annual and peak summer temperatures increased. Further, rainbow trout size decreased by ~29 mm with an earlier onset of cold winter temperatures. While increased recruitment may be the more likely outcome of a warmer and drier climate, density-dependent growth constraints could exacerbate temperature-dependent growth reductions. As such, managers may consider implementing flows to reduce recruitment or altering infrastructure to maintain coldwater reservoir releases.

  2. Simulation of streamflow temperatures in the Yakima River basin, Washington, April-October 1981

    USGS Publications Warehouse

    Vaccaro, J.J.

    1986-01-01

    The effects of storage, diversion, return flow, and meteorological variables on water temperature in the Yakima River, in Washington State, were simulated, and the changes in water temperature that could be expected under four alternative-management scenarios were examined for improvement in anadromous fish environment. A streamflow routing model and Lagrangian streamflow temperature model were used to simulate water discharge and temperature in the river. The estimated model errors were 12% for daily discharge and 1.7 C for daily temperature. Sensitivity analysis of the simulation of water temperatures showed that the effect of reservoir outflow temperatures diminishes in a downstream direction. A 4 C increase in outflow temperatures results in a 1.0 C increase in mean irrigation season water temperature at Umtanum in the upper Yakima River basin, but only a 0.01C increase at Prosser in the lower basin. The influence of air temperature on water temperature increases in a downstream direction and is the dominant influence in the lower basin. A 4 C increase in air temperature over the entire basin resulted in a 2.34 C increase in river temperatures at Prosser in the lower basin and 1.46 C at Umtanum in the upper basin. Changes in wind speed and model wind-function parameters had little effect on the model predicted water temperature. Of four alternative management scenarios suggested by the U.S. Bureau of Indian Affairs and the Yakima Indian Nation, the 1981 reservoir releases maintained without diversions or return flow in the river basin produced water temperatures nearest those considered as preferable for salmon and steelhead trout habitat. The alternative management scenario for no reservoir storage and no diversions or return flows in the river basin (estimate of natural conditions) produced conditions that were the least like those considered as preferable for salmon and steelhead trout habitat. (Author 's abstract)

  3. Frozen breast milk at -20 degrees C and -80 degrees C: a longitudinal study of glutathione peroxidase activity and malondialdehyde concentration.

    PubMed

    Silvestre, Dolores; Miranda, María; Muriach, María; Almansa, Inmaculada; Jareño, Enrique; Romero, Francisco J

    2010-02-01

    When breast milk extraction and storage is required before ingestion, it is important to establish the conditions that ensure the least losses in milk quality, like the antioxidant capacity. The present study evaluates glutathione peroxidase activity and malondialdehyde concentration of breast milk when stored frozen, comparing the effects of 2 temperatures (-20 degrees C and -80 degrees C) and different storage times (15, 30, and 60 days). The results indicate that freezing induces losses in the antioxidant properties of breast milk and that such losses increase with the duration of storage and differ in intensity according to the temperature. It is concluded that to maximally preserve the antioxidant properties of breast milk, it is advisable to store the latter at -80 degrees C for a period of less than 30 days, rather than for shorter time periods at the usual temperature of -20 degrees C.

  4. Thermal Storage Properties of Molten Nitrate Salt-Based Nanofluids with Graphene Nanoplatelets.

    PubMed

    Xie, Qiangzhi; Zhu, Qunzhi; Li, Yan

    2016-12-01

    In this study, the effect of concentration of nanoparticles on the thermal storage properties of molten nitrate salt-based nanofluids with graphene nanoplatelets (GNPs) was investigated. Solar salt consisting of sodium nitrate and potassium nitrate was utilized as the base material for the nanofluids. Homogeneous dispersion of GNPs within the solar salt was observed through scanning electron microscopy analysis. For both solar salt and resultant nanofluids, differential scanning calorimetry was employed to measure the thermal storage properties, including characteristic temperatures of phase change, startup heat, and specific heat capacity (SHC). A maximum increase of 16.7 % in SHC at the liquid phase was found at an optimal concentration of 1 wt% of GNPs. At the same concentration, the onset temperature decreased by 10.4 °C, the endset temperature decreased by 4.7 °C, and the startup heat decreased by 9 %.

  5. Metabolomic profiling of beer reveals effect of temperature on non-volatile small molecules during short-term storage.

    PubMed

    Heuberger, Adam L; Broeckling, Corey D; Lewis, Matthew R; Salazar, Lauren; Bouckaert, Peter; Prenni, Jessica E

    2012-12-01

    The effect of temperature on non-volatile compounds in beer has not been well characterised during storage. Here, a metabolomics approach was applied to characterise the effect of storage temperature on non-volatile metabolite variation after 16weeks of storage, using fresh beer as a control. The metabolite profile of room temperature stored (RT) and cold temperature stored (CT) beer differed significantly from fresh, with the most substantial variation observed between RT and fresh beer. Metabolites that changed during storage included prenylated flavonoids, purines, and peptides, and all showed reduced quantitative variation under the CT storage conditions. Corresponding sensory panel observations indicated significant beer oxidation after 12 and 16weeks of storage, with higher values reported for RT samples. These data support that temperature affected beer oxidation during short-term storage, and reveal 5-methylthioadenosine (5-MTA) as a candidate non-volatile metabolite marker for beer oxidation and staling. Copyright © 2012 Elsevier Ltd. All rights reserved.

  6. Heat of adsorption, adsorption stress, and optimal storage of methane in slit and cylindrical carbon pores predicted by classical density functional theory.

    PubMed

    Hlushak, Stepan

    2018-01-03

    Temperature, pressure and pore-size dependences of the heat of adsorption, adsorption stress, and adsorption capacity of methane in simple models of slit and cylindrical carbon pores are studied using classical density functional theory (CDFT) and grand-canonical Monte-Carlo (MC) simulation. Studied properties depend nontrivially on the bulk pressure and the size of the pores. Heat of adsorption increases with loading, but only for sufficiently narrow pores. While the increase is advantageous for gas storage applications, it is less significant for cylindrical pores than for slits. Adsorption stress and the average adsorbed fluid density show oscillatory dependence on the pore size and increase with bulk pressure. Slit pores exhibit larger amplitude of oscillations of the normal adsorption stress with pore size increase than cylindrical pores. However, the increase of the magnitude of the adsorption stress with bulk pressure increase is more significant for cylindrical than for slit pores. Adsorption stress appears to be negative for a wide range of pore sizes and external conditions. The pore size dependence of the average delivered density of the gas is analyzed and the optimal pore sizes for storage applications are estimated. The optimal width of slit pore appears to be almost independent of storage pressure at room temperature and pressures above 10 bar. Similarly to the case of slit pores, the optimal radius of cylindrical pores does not exhibit much dependence on the storage pressure above 15 bar. Both optimal width and optimal radii of slit and cylindrical pores increase as the temperature decreases. A comparison of the results of CDFT theory and MC simulations reveals subtle but important differences in the underlying fluid models employed by the approaches. The differences in the high-pressure behaviour between the hard-sphere 2-Yukawa and Lennard-Jones models of methane, employed by the CDFT and MC approaches, respectively, result in an overestimation of the heat of adsorption by the CDFT theory at higher loadings. However, both adsorption stress and adsorption capacity appear to be much less sensitive to the differences between the models and demonstrate excellent agreement between the theory and the computer experiment.

  7. [Physical and chemical evaluation during refrigeration storage of salted catfish (Pseudoplatystoma sp.) in brine solution, and packed under vacuum].

    PubMed

    Rodríguez, Diana; Barrero, Marinela; Kodaira, Makie

    2009-06-01

    Salting fish in the south Venezuelan towns are still the main method of preserving fish including cutt, and salting fish process, storage and commercialization. As the result, salted-dried fish is particularly susceptible to spoilage by a number of factors, including lipid oxidation, browning meat. Packing salted fish product is an alternative increasing storage life time reducing lost of quality and enhancing the storage time. The present study evaluated the physic, chemist, and sensory quality of fish fillet from cat fish (Pseudoplatystoma sp.) from Apure state, Venezuela. Fillet fish were placed in brine solution at 36% of sodium chloride 1:2 fillet: brine solution; after, they were packed under followed conditions: vacuum, vacuum and storage under refrigeration condition, and room temperature. The results showed significant differences (p < 0.01) for moisture, salt content, and Aw. The fillets packed at vacuum and storage at 4 degrees C were significant different from the resting treatments; not significant differences were presented at room and refrigeration temperature after three moths. The best conditions treatment was vacuum packing and refrigeration at 4 degrees C.

  8. Physicochemical and microbiological changes during the refrigerated storage of lamb loins sous-vide cooked at different combinations of time and temperature.

    PubMed

    Roldán, Mar; Antequera, Teresa; Hernández, Alejandro; Ruiz, Jorge

    2015-10-01

    This study aimed to determine the influence of cooking temperature (either 60 ℃ or 80 ℃) and time (6 h or 24 h) on the physicochemical (weight loss, moisture content, instrumental color, instrumental texture, lipid and protein oxidation) and microbiological changes underwent by sous-vide cooked lamb loins during refrigerated storage for 30 days. There was a slight trend to decreasing weight losses in some of the cooking treatments, but only in samples cooked at 60 ℃ for 6 h it was paralleled with an increase in moisture content. The only noteworthy oxidative change was a marked decrease in conjugated dienes after two weeks of storage in samples cooked at 80 ℃ for 24 h. Neither instrumental texture nor color showed noticeable variations during the storage. Microbial population remained quite low during the whole refrigerated storage. Overall, most of the studied parameters showed only scarce changes throughout 30 days of refrigerated storage that most likely would not influence the quality of sous-vide cooked loin lambs. © The Author(s) 2014.

  9. Experimental investigation on the thermal performance of heat storage walls coupled with active solar systems

    NASA Astrophysics Data System (ADS)

    Zhao, Chunyu; You, Shijun; Zhu, Chunying; Yu, Wei

    2016-12-01

    This paper presents an experimental investigation of the performance of a system combining a low-temperature water wall radiant heating system and phase change energy storage technology with an active solar system. This system uses a thermal storage wall that is designed with multilayer thermal storage plates. The heat storage material is expanded graphite that absorbs a mixture of capric acid and lauric acid. An experiment is performed to study the actual effect. The following are studied under winter conditions: (1) the temperature of the radiation wall surface, (2) the melting status of the thermal storage material in the internal plate, (3) the density of the heat flux, and (4) the temperature distribution of the indoor space. The results reveal that the room temperature is controlled between 16 and 20 °C, and the thermal storage wall meets the heating and temperature requirements. The following are also studied under summer conditions: (1) the internal relationship between the indoor temperature distribution and the heat transfer within the regenerative plates during the day and (2) the relationship between the outlet air temperature and inlet air temperature in the thermal storage wall in cooling mode at night. The results indicate that the indoor temperature is approximately 27 °C, which satisfies the summer air-conditioning requirements.

  10. Long-Term Viscoelastic Response of E-glass/Bismaleimide Composite in Seawater Environment

    NASA Astrophysics Data System (ADS)

    Yian, Zhao; Zhiying, Wang; Keey, Seah Leong; Boay, Chai Gin

    2015-12-01

    The effect of seawater absorption on the long-term viscoelastic response of E-glass/BMI composite is presented in this paper. The diffusion of seawater into the composite shows a two-stage behavior, dominated by Fickian diffusion initially and followed by polymeric relaxation. The Glass transition temperature (Tg) of the composite with seawater absorption is considerably lowered due to the plasticization effect. However the effect of water absorption at 50 °C is found to be reversible after drying process. The time-temperature superposition (TTS) was performed based on the results of Dynamic Mechanical Analysis to construct the master curve of storage modulus. The shift factors exhibit Arrhenius behavior when temperature is well below Tg and Vogel-Fulcher-Tammann (VFT) like behavior when temperature gets close to glass transition region. As a result, a semi-empirical formulation is proposed to account for the seawater absorption effect in predicting long-term viscoelastic response of BMI composites based on temperature dependent storage modulus and TTS. The predicted master curves show that the degradation of storage modulus accelerates with both seawater exposure and increasing temperature. The proposed formulation can be applied to predict the long-term durability of any thermorheologically simple composite materials in seawater environment.

  11. Large-Scale Wireless Temperature Monitoring System for Liquefied Petroleum Gas Storage Tanks.

    PubMed

    Fan, Guangwen; Shen, Yu; Hao, Xiaowei; Yuan, Zongming; Zhou, Zhi

    2015-09-18

    Temperature distribution is a critical indicator of the health condition for Liquefied Petroleum Gas (LPG) storage tanks. In this paper, we present a large-scale wireless temperature monitoring system to evaluate the safety of LPG storage tanks. The system includes wireless sensors networks, high temperature fiber-optic sensors, and monitoring software. Finally, a case study on real-world LPG storage tanks proves the feasibility of the system. The unique features of wireless transmission, automatic data acquisition and management, local and remote access make the developed system a good alternative for temperature monitoring of LPG storage tanks in practical applications.

  12. The stability of water- and fat-soluble vitamin in dentifrices according to pH level and storage type.

    PubMed

    Park, Jung-Eun; Kim, Ki-Eun; Choi, Yong-Jun; Park, Yong-Duk; Kwon, Ha-Jeong

    2016-02-01

    The purpose of this study is to evaluate the vitamin stabilities in dentifrices by analyzing various vitamins according to the level and storage temperature. The stabilities of water- and fat-soluble vitamins were investigated in buffer solution at different pH values (4, 7, 8, 10 and 11) for 14 days and in dentifrices at different pH (7 and 10) for 5 months at two temperature conditions (room and refrigeration temperature) by analyzing the remaining amounts using HPLC methods. In the buffer solution, the stability of vitamins B1 , B6 and C was increased as the pH values increased. Vitamins E and K showed poor stability at pH 4, and vitamin B3 showed poor stability at pH 11. In dentifrices, the storage temperature highly influenced vitamin stability, especially vitamins C and E, but the stabilities of vitamins B1 and C according to pH values did not correspond to the buffer solution tests. Vitamin B group was relatively stable in dentifrices, but vitamin C completely disappeared after 5 months. Vitamin K showed the least initial preservation rates. Vitamins were not detected in commercial dentifrices for adults and detected amounts were less than the advertised contents in dentifrices for children. Copyright © 2015 John Wiley & Sons, Ltd.

  13. Formation, antioxidant property and oxidative stability of cold pressed rice bran oil emulsion.

    PubMed

    Thanonkaew, Amonrat; Wongyai, Surapote; Decker, Eric A; McClements, David J

    2015-10-01

    Cold pressed rice bran oil (CPRBO) is used in foods, cosmetics, and pharmaceuticals due to its desirable health and functional attributes. The purpose of this work was to study the formation, antioxidant property and oxidative stability of oil-in-water emulsion of CPRBO. The influence of oil (10-40 % CPRBO) and surfactant (1-5 % glyceryl monostearate (GMS)) concentration on the properties of emulsions were studied. The lightness (L*) and yellowness (b*) of CPRBO emulsions decreased as GMS concentration increased, which was attributed to a decrease in droplet size after homogenization. The CPRBO emulsion was stable during storage at room temperature for 30 days. Increasing the oil concentration in the CPRBO emulsions increased their antioxidant activity, which can be attributed to the corresponding increase in phytochemical content. However, GMS concentration had little impact on the antioxidant activity of CPRBO emulsions. The storage of CPRBO emulsion at room temperature showed that lipid oxidation markers gradually increased after 30 days of storage, which was correlated to a decrease in gamma oryzanol content and antioxidant activity. These results have important implications for the utilization of rice bran oil (RBO) as a function ingredient in food, cosmetic, and pharmaceutical products.

  14. Optimizing blood collection, transport and storage conditions for cell free DNA increases access to prenatal testing.

    PubMed

    Wong, David; Moturi, Sharmili; Angkachatchai, Vach; Mueller, Reinhold; DeSantis, Grace; van den Boom, Dirk; Ehrich, Mathias

    2013-08-01

    Fetal mutations and fetal chromosomal abnormalities can be detected by molecular analysis of circulating cell free fetal DNA (ccffDNA) from maternal plasma. This comprehensive study was aimed to investigate and verify blood collection and blood shipping conditions that enable Noninvasive Prenatal Testing. Specifically, the impact of shipping and storage on the stability and concentration of circulating cell-free DNA (ccfDNA) in Streck® Cell-Free DNA™ Blood Collection Tubes (Streck BCTs, Streck, Omaha NE). These BCTs were designed to minimize cellular degradation, and thus effectively prevent dilution of fetal ccf DNA by maternal genomic DNA, was evaluated. Peripheral venous maternal blood was collected into Streck BCTs to investigate four aspects of handling and processing conditions: (1) time from blood draw to plasma processing; (2) storage temperature; (3) mechanical stress; and (4) lot-to-lot tube variations. Maternal blood stored in Streck BCTs for up to 7 days at ambient temperature provides stable concentrations of ccffDNA. The amount of fetal DNA did not change over a broad range of storage temperatures (4°C, 23°C, 37°C, 40°C), but the amount of total (largely maternal) DNA increased in samples stored at 23°C and above, indicating maternal cell degradation and genomic DNA release at elevated temperatures. Shipping maternal blood in Streck BCTs, did not affect sample quality. Maternal plasma DNA stabilized for 0 to 7 days in Streck BCTs can be used for non-invasive prenatal molecular applications, when temperatures are maintained within the broad parameters assessed in this study. Copyright © 2013 The Authors. Published by Elsevier Inc. All rights reserved.

  15. 40 CFR Table 21 to Subpart G of... - Average Storage Temperature (Ts) as a Function of Tank Paint Color

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 10 2012-07-01 2012-07-01 false Average Storage Temperature (Ts) as a..., and Wastewater Pt. 63, Subpt. G, Table 21 Table 21 to Subpart G of Part 63—Average Storage Temperature (Ts) as a Function of Tank Paint Color Tank Color Average Storage Temperature (Ts) White TA a = 0...

  16. 40 CFR Table 21 to Subpart G of... - Average Storage Temperature (Ts) as a Function of Tank Paint Color

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 9 2010-07-01 2010-07-01 false Average Storage Temperature (Ts) as a..., and Wastewater Pt. 63, Subpt. G, Table 21 Table 21 to Subpart G of Part 63—Average Storage Temperature (Ts) as a Function of Tank Paint Color Tank Color Average Storage Temperature (Ts) White TA a = 0...

  17. 40 CFR Table 21 to Subpart G of... - Average Storage Temperature (Ts) as a Function of Tank Paint Color

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 10 2013-07-01 2013-07-01 false Average Storage Temperature (Ts) as a..., and Wastewater Pt. 63, Subpt. G, Table 21 Table 21 to Subpart G of Part 63—Average Storage Temperature (Ts) as a Function of Tank Paint Color Tank Color Average Storage Temperature (Ts) White TA a = 0...

  18. 40 CFR Table 21 to Subpart G of... - Average Storage Temperature (Ts) as a Function of Tank Paint Color

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 9 2011-07-01 2011-07-01 false Average Storage Temperature (Ts) as a..., and Wastewater Pt. 63, Subpt. G, Table 21 Table 21 to Subpart G of Part 63—Average Storage Temperature (Ts) as a Function of Tank Paint Color Tank Color Average Storage Temperature (Ts) White TA a = 0...

  19. 40 CFR Table 21 to Subpart G of... - Average Storage Temperature (Ts) as a Function of Tank Paint Color

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 10 2014-07-01 2014-07-01 false Average Storage Temperature (Ts) as a..., and Wastewater Pt. 63, Subpt. G, Table 21 Table 21 to Subpart G of Part 63—Average Storage Temperature (Ts) as a Function of Tank Paint Color Tank Color Average Storage Temperature (Ts) White TA a = 0...

  20. Caspase inhibitors increase the rate of recovery of neural stem/progenitor cells from post-mortem rat brains stored at room temperature.

    PubMed

    Hasegawa, Atsuko; Yamada, Chikako; Tani, Miho; Hirano, Shun-ichiro; Tokumoto, Yasuhito; Miyake, Jun

    2009-06-01

    To match the demand of regenerative medicine for nerve system, collection of stem cells from the post-mortem body is one of the most practical ways. In this study, the storage condition of the post-mortem body was examined. We prepared neural stem/progenitor cells (NSPCs) from post-mortem rat brains stored at different temperatures. When brains were stored at 4 degrees C, for one week, we were able to obtain neurospheres (a spheroid body containing NSPCs) by stimulation of cells with epidermal growth factor (EGF). Incremental increases in storage temperature decreased the rate of appearance of neurospheres. Within 48 h at 15 degrees C, 24 h at 25 degrees C, in both condition, we were able to recover NSPCs from post-mortem rat brains. At 15 degrees C, 90% of neurosphere-forming activity was lost within 24 h. However, even after 24 h at 25 degrees C, 2% neurosphere-forming activity remained. After 6 h of death, there was very little difference between the rates of NSPC recovery at 4 degrees C and 25 degrees C. Addition of caspase inhibitors to both the rat brain storage solution and the NSPC culture medium increased the rate of neurosphere-forming activity. In particular, an inhibitor of caspase-8 activity increased the NSPC recovery rate approximately three-fold, with no accompanying detrimental effects on neural differentiation in vitro.

  1. Responses of fresh-cut products of four mango cultivars under two different storage conditions.

    PubMed

    Sharma, Sonu; Rao, Tadapaneni Venkata Ramana

    2017-05-01

    Due to availability of minimally processed products, the consumption of fresh produce has increased over recent years. The present study has been undertaken with the objective of screening of four mango cultivars ('Kesar', 'Rajapuri', 'Totapuri' and 'Ladvo') for evaluating the consequences of minimal processing on their quality attributes under storage at two different temperatures (5 ± 1 °C, 95% RH and 10 ± 1 °C, 87% RH) up to 12 days. The result of the study revealed significant impacts of low temperature storage on the quality parameters of fresh-cut mango cultivars. The evaluated bioactive compounds such as total phenolics, vitamin C and carotenoids were better retained in the samples stored at 5 °C as compared with that of 10 °C. Moreover, the storage of fresh-cut mango cultivars at 5 °C showed lower water loss and microbial contamination. Sensory analyses revealed that the storage of fresh-cut mango cultivars at 10 °C influenced overall acceptability due to changes in their visual perception, though taste, odor and firmness were less affected. This study revealed a significant variation in the storability of fresh-cut mango cultivars with respect to the storage temperature. Among currently studied four cultivars of mango, slices of 'Totapuri' showed comparatively the least change in color, firmness and sensory properties during storage at 5 and 10 °C and it can be a potential cultivar for fresh-cut processing.

  2. Modeling the wet bulb globe temperature using standard meteorological measurements.

    PubMed

    Liljegren, James C; Carhart, Richard A; Lawday, Philip; Tschopp, Stephen; Sharp, Robert

    2008-10-01

    The U.S. Army has a need for continuous, accurate estimates of the wet bulb globe temperature to protect soldiers and civilian workers from heat-related injuries, including those involved in the storage and destruction of aging chemical munitions at depots across the United States. At these depots, workers must don protective clothing that increases their risk of heat-related injury. Because of the difficulty in making continuous, accurate measurements of wet bulb globe temperature outdoors, the authors have developed a model of the wet bulb globe temperature that relies only on standard meteorological data available at each storage depot for input. The model is composed of separate submodels of the natural wet bulb and globe temperatures that are based on fundamental principles of heat and mass transfer, has no site-dependent parameters, and achieves an accuracy of better than 1 degree C based on comparisons with wet bulb globe temperature measurements at all depots.

  3. Fate of Vibrio parahaemolyticus on shrimp after acidic electrolyzed water treatment.

    PubMed

    Wang, Jing Jing; Sun, Wen Shuo; Jin, Meng Tong; Liu, Hai Quan; Zhang, Weijia; Sun, Xiao Hong; Pan, Ying Jie; Zhao, Yong

    2014-06-02

    The objective of this study was to investigate the fate of Vibrio parahaemolyticus on shrimp after acidic electrolyzed water (AEW) treatment during storage. Shrimp, inoculated with a cocktail of four strains of V. parahaemolyticus, were stored at different temperatures (4-30 °C) after AEW treatment. Experimental data were fitted to modified Gompertz and Log-linear models. The fate of V. parahaemolyticus was determined based on the growth and survival kinetics parameters (lag time, λ; the maximum growth rate, μmax; the maximum growth concentration, D; the inactivation value, K) depending on the respective storage conditions. Moreover, real-time PCR was employed to study the population dynamics of this pathogen during the refrigeration temperature storage (10, 7, 4 °C). The results showed that AEW treatment could markedly (p<0.05) decrease the growth rate (μmax) and extend the lag time (λ) during the post-treatment storage at 30, 25, 20 and 15 °C, while it did not present a capability to lower the maximum growth concentration (D). AEW treatment increased the sensitivity of V. parahaemolyticus to refrigeration temperatures, indicated by a higher (p<0.05) inactivation value (K) of V. parahaemolyticus, especially for 10 °C storage. The results also revealed that AEW treatment could completely suppress the proliferation of V. parahaemolyticus in combination with refrigeration temperature. Based on above analysis, the present study demonstrates the potential of AEW in growth inhibition or death acceleration of V. parahaemolyticus on seafood, hence to greatly reduce the risk of illness caused by this pathogen during post-treatment storage. Copyright © 2014 Elsevier B.V. All rights reserved.

  4. Preliminary investigation of thermal behaviour of PCM based latent heat thermal energy storage

    NASA Astrophysics Data System (ADS)

    Pop, Octavian G.; Fechete Tutunaru, Lucian; Bode, Florin; Balan, Mugur C.

    2018-02-01

    Solid-liquid phase change is used to accumulate and release cold in latent heat thermal energy storage (LHTES) in order to reduce energy consumption of air cooling system in buildings. The storing capacity of the LHTES depends greatly on the exterior air temperatures during the summer nights. One approach in intensifying heat transfer is by increasing the air's velocity. A LHTES was designed to be integrated in the air cooling system of a building located in Bucharest, during the month of July. This study presents a numerical investigation concerning the impact of air inlet temperatures and air velocity on the formation of solid PCM, on the cold storing capacity and energy consumption of the LHTES. The peak amount of accumulated cold is reached at different air velocities depending on air inlet temperature. For inlet temperatures of 14°C and 15°C, an increase of air velocity above 50% will not lead to higher amounts of cold being stored. For Bucharest during the hottest night of the year, a 100 % increase in air velocity will result in 5.02% more cold being stored, at an increase in electrical energy consumption of 25.30%, when compared to the reference values.

  5. Thermally responsive polymer electrolytes for inherently safe electrochemical energy storage

    NASA Astrophysics Data System (ADS)

    Kelly, Jesse C.

    Electrochemical double layer capacitors (EDLCs), supercapacitors and Li-ion batteries have emerged as premier candidates to meet the rising demands in energy storage; however, such systems are limited by thermal hazards, thermal runaway, fires and explosions, all of which become increasingly more dangerous in large-format devices. To prevent such scenarios, thermally-responsive polymer electrolytes (RPEs) that alter properties in electrochemical energy storage devices were designed and tested. These RPEs will be used to limit or halt device operation when temperatures increase beyond a predetermined threshold, therefore limiting further heating. The development of these responsive systems will offer an inherent safety mechanism in electrochemical energy storage devices, while preserving the performance, lifetimes, and versatility that large-format systems require. Initial work focused on the development of a model system that demonstrated the concept of RPEs in an electrochemical device. Aqueous electrolyte solutions of polymers exhibiting properties that change in response to temperature were developed for applications in EDLCs and supercapacitors. These "smart materials" provide a means to control electrochemical systems where polymer phase separation at high temperatures affects electrolyte properties and inhibits device performance. Aqueous RPEs were synthesized using N-isopropylacrylamide, which governs the thermal properties, and fractions of acrylic acid or vinyl sulfonic acids, which provide ions to the solution. The molecular properties of these aqueous RPEs, specifically the ionic composition, were shown to influence the temperature-dependent electrolyte properties and the extent to which these electrolytes control the energy storage characteristics of a supercapacitor device. Materials with high ionic content provided the highest room temperature conductivity and electrochemical activity; however, RPEs with low ionic content provided the highest "on-off" ratio in electrochemical activity at elevated temperatures. Overall, solution pH and conductivity were altered by an order of magnitude and device performance (ability to store charge) decreased by over 70%. After demonstration of a model responsive electrolyte in an aqueous system, ionic liquid (IL) based electrolytes were developed as a means of controlling the electrochemical performance in the non-aqueous environments that batteries, specifically Li-ion, require. Here, two systems were developed: (1) an electrolyte comprising poly(ethylene oxide) (PEO), the IL, [EMIM][BF4], and a lithium salt and (2) an electrolyte comprising poly(benzyl methacrylate) (PBzMA), the IL, [EMIM][TFSI], and a lithium salt. In each system, the polymer-IL phase separation inhibited device operation at elevated temperatures. For the PEO/IL electrolyte, the thermally induced liquid-liquid phase separation was shown to decrease the ionic conductivity, thereby affecting the concentration of ions at the electrode. Additionally, an increasing charge transfer resistance associated with the phase separated polymer coating the porous electrode was shown to limit electrochemical activity significantly. For the PBzMA/IL electrolyte, the solid-liquid phase separation did not show a change in conductivity, but did cause a drastic increase in charge transfer resistance, effectively shutting off Li-ion battery operation at high temperatures. Such responsive mixtures provide a transformative approach to regulating electrochemical processes, which is necessary to achieve inherently safe operation in large format energy storage with EDLCs, supercapacitors and Li-ion batteries.

  6. Effect of temperature towards lipid oxidation and non-enzymatic browning reactions in krill oil upon storage.

    PubMed

    Lu, F S H; Bruheim, I; Haugsgjerd, B O; Jacobsen, C

    2014-08-15

    The main objective of this study was to investigate the effect of temperature towards lipid oxidation and non-enzymatic browning reactions in krill oil upon storage. Krill oil was incubated at two different temperatures (20 and 40 °C) for 28 or 42 days. The oxidative stability of krill oil was assessed by peroxide value and anisidine value, measurement of lipid derived volatiles, lipid classes and antioxidants. The non-enzymatic browning reactions were assessed through the measurement of pyrroles, free amino acids content and Strecker-derived volatiles. The increase of incubation temperature firstly increased the lipid oxidation in krill oil and subsequently the non-enzymatic browning reactions. The occurrence of these reactions was most likely due to the reaction between α-dicarbonyl or carbonyl compounds with amino acids or ammonia. In addition to tocopherol and astaxanthin esters, the formation of pyrroles might help to protect the krill oil against lipid oxidation. Copyright © 2014 Elsevier Ltd. All rights reserved.

  7. Energy Dissipation in Ex-Vivo Porcine Liver during Electrosurgery

    PubMed Central

    Karaki, Wafaa; Akyildiz, Ali; De, Suvranu

    2017-01-01

    This paper explores energy dissipation in ex-vivo liver tissue during radiofrequency current excitation with application in electrosurgery. Tissue surface temperature for monopolar electrode configuration is measured using infrared thermometry. The experimental results are fitted to a finite element model for transient heat transfer taking into account energy storage and conduction in order to extract information about “apparent” specific heat, which encompasses storage and phase change. The average apparent specific heat determined for low temperatures is in agreement with published data. However, at temperatures approaching the boiling point of water, apparent specific heat increases by a factor of five, indicating that vaporization plays an important role in the energy dissipation through latent heat loss. PMID:27479955

  8. Migration kinetics of mineral oil hydrocarbons from recycled paperboard to dry food: monitoring of two real cases.

    PubMed

    Lorenzini, R; Biedermann, M; Grob, K; Garbini, D; Barbanera, M; Braschi, I

    2013-01-01

    Mineral oil hydrocarbons present in printing inks and recycled paper migrate from paper-based food packaging to foods primarily through the gas phase. Migration from two commercial products packed in recycled paperboard, i.e. muesli and egg pasta, was monitored up to the end of their shelf life (1 year) to study the influence of time, storage conditions, food packaging structure and temperature. Mineral oil saturated and aromatic hydrocarbons (MOSH and MOAH, respectively), and diisopropyl naphthalenes (DIPN) were monitored using online HPLC-GC/FID. Storage conditions were: free standing, shelved, and packed in transport boxes of corrugated board, to represent domestic, supermarket and warehouse storage, respectively. Migration to food whose packs were kept in transport boxes was the highest, especially after prolonged storage, followed by shelved and free-standing packs. Tested temperatures were representative of refrigeration, room temperature, storage in summer months and accelerated migration testing. Migration was strongly influenced by temperature: for egg pasta directly packed in paperboard, around 30 mg kg⁻¹ of MOSH migrated in 8 months at 20°C, but in only 1 week at 40°C. Muesli was contained into an internal polyethylene bag, which firstly adsorbed hydrocarbons and later released them partly towards the food. Differently, the external polypropylene bag, containing pasta and recycled paper tray, strongly limited the migration towards the atmosphere and gave rise to the highest level of food contamination. Tests at increased temperatures not only accelerated migration, but also widened the migration of hydrocarbons to higher molecular masses, highlighting thus a difficult interpretation of data from accelerated simulation.

  9. Cryo-Milling and the Hydrogen Storage Properties of NaAlH4

    NASA Astrophysics Data System (ADS)

    Feller, Kevin; Dobbins, Tabbetha

    2013-03-01

    High energy ball milling of metal hydrides is a common way to both introduce catalysts (e.g. TiCl3) and to simultaneously increase the surface area. Both catalysis and increased surface area improve hydrogen storage capacity of the material. Nanostructuring of hydrides by depositing them into mesoporous templates (such as anodized alumina, MOFs, and SBA-15) has become a common way to increase surface area. However, the mesoporous template does not add hydrogen storage capacity--and thus, tends to decreased overall storage weight percent for the nanostructured hydride material. As with most materials, hydrides become brittle at low temperatures and will tend to fracture more readily. We will process Sodium Aluminum Hydride (NaAlH4) using cryogenic high energy ball milling using an in-house modified chamber SPEX Certiprep M8000 mixer/mill in order to gain a nanostructured hydride without mesoporous template material. Details of the modified mixer mill design will be presented. Ultimately, our planned future work is to study the resultant material using x-ray diffraction (Scherrer method for crystallite size), absorption/desorption temperature programmed desorption (TPD), and ultrasmall-angle x-ray scattering (USAXS) microstructural quantification to understand the role of cryomilling on enhancing the material's ability to store (and release) hydrogen.

  10. Can storage reduce electricity consumption? A general equation for the grid-wide efficiency impact of using cooling thermal energy storage for load shifting

    NASA Astrophysics Data System (ADS)

    Deetjen, Thomas A.; Reimers, Andrew S.; Webber, Michael E.

    2018-02-01

    This study estimates changes in grid-wide, energy consumption caused by load shifting via cooling thermal energy storage (CTES) in the building sector. It develops a general equation for relating generator fleet fuel consumption to building cooling demand as a function of ambient temperature, relative humidity, transmission and distribution current, and baseline power plant efficiency. The results present a graphical sensitivity analysis that can be used to estimate how shifting load from cooling demand to cooling storage could affect overall, grid-wide, energy consumption. In particular, because power plants, air conditioners and transmission systems all have higher efficiencies at cooler ambient temperatures, it is possible to identify operating conditions such that CTES increases system efficiency rather than decreasing it as is typical for conventional storage approaches. A case study of the Dallas-Fort Worth metro area in Texas, USA shows that using CTES to shift daytime cooling load to nighttime cooling storage can reduce annual, system-wide, primary fuel consumption by 17.6 MWh for each MWh of installed CTES capacity. The study concludes that, under the right circumstances, cooling thermal energy storage can reduce grid-wide energy consumption, challenging the perception of energy storage as a net energy consumer.

  11. Effect of semen extender and storage temperature on ram sperm motility over time

    USDA-ARS?s Scientific Manuscript database

    Storage of ram semen for long period of time depends on a number of factors, including type of extender and storage temperature. A study compared the effect of semen extender and storage temperature on motility of ram semen stored for 72 h. Semen collected via electroejaculator from 5 mature Katahd...

  12. Chilling Stress Upregulates α-Linolenic Acid-Oxidation Pathway and Induces Volatiles of C6 and C9 Aldehydes in Mango Fruit.

    PubMed

    Sivankalyani, Velu; Maoz, Itay; Feygenberg, Oleg; Maurer, Dalia; Alkan, Noam

    2017-01-25

    Mango-fruit storage period and shelf life are prolonged by cold storage. However, chilling temperature induces physiological and molecular changes, compromising fruit quality. In our previous transcriptomic study of mango fruit, cold storage at suboptimal temperature (5 °C) activated the α-linolenic acid metabolic pathway. To evaluate changes in fruit quality during chilling, we analyzed mango "Keitt" fruit peel volatiles. GC-MS analysis revealed significant modulations in fruit volatiles during storage at suboptimal temperature. Fewer changes were seen in response to the time of storage. The mango volatiles related to aroma, such as δ-3-carene, (Z)-β-ocimene, and terpinolene, were downregulated during the storage at suboptimal temperature. In contrast, C 6 and C 9 aldehydes and alcohols-α-linolenic acid derivatives 1-hexanal, (Z)-3-hexenal, (Z)-3-hexenol, (E)-2-hexenal, and nonanal-were elevated during suboptimal-temperature storage, before chilling-injury symptoms appeared. Detection of those molecules before chilling symptoms could lead to a new agro-technology to avoid chilling injuries and maintain fruit quality during cold storage at the lowest possible temperature.

  13. Large-Scale Wireless Temperature Monitoring System for Liquefied Petroleum Gas Storage Tanks

    PubMed Central

    Fan, Guangwen; Shen, Yu; Hao, Xiaowei; Yuan, Zongming; Zhou, Zhi

    2015-01-01

    Temperature distribution is a critical indicator of the health condition for Liquefied Petroleum Gas (LPG) storage tanks. In this paper, we present a large-scale wireless temperature monitoring system to evaluate the safety of LPG storage tanks. The system includes wireless sensors networks, high temperature fiber-optic sensors, and monitoring software. Finally, a case study on real-world LPG storage tanks proves the feasibility of the system. The unique features of wireless transmission, automatic data acquisition and management, local and remote access make the developed system a good alternative for temperature monitoring of LPG storage tanks in practical applications. PMID:26393596

  14. Enhanced annealing stability and perpendicular magnetic anisotropy in perpendicular magnetic tunnel junctions using W layer

    NASA Astrophysics Data System (ADS)

    Chatterjee, Jyotirmoy; Sousa, Ricardo C.; Perrissin, Nicolas; Auffret, Stéphane; Ducruet, Clarisse; Dieny, Bernard

    2017-05-01

    The magnetic properties of the perpendicular storage electrode (buffer/MgO/FeCoB/Cap) were studied as a function of annealing temperature by replacing Ta with W and W/Ta cap layers with variable thicknesses. W in the cap boosts up the annealing stability and increases the effective perpendicular anisotropy by 30% compared to the Ta cap. Correspondingly, an increase in the FeCoB critical thickness characterizing the transition from perpendicular to in-plane anisotropy was observed. Thicker W layer in the W(t)/Ta 1 nm cap layer makes the storage electrode highly robust against annealing up to 570 °C. The stiffening of the overall stack resulting from the W insertion due to its very high melting temperature seems to be the key mechanism behind the extremely high thermal robustness. The Gilbert damping constant of FeCoB with the W/Ta cap was found to be lower when compared with the Ta cap and stable with annealing. The evolution of the magnetic properties of bottom pinned perpendicular magnetic tunnel junctions (p-MTJ) stack with the W2/Ta1 nm cap layer shows back-end-of-line compatibility with increasing tunnel magnetoresistance up to the annealing temperature of 425 °C. The pMTJ thermal budget is limited by the synthetic antiferromagnetic hard layer which is stable up to 425 °C annealing temperature while the storage layer is stable up to 455 °C.

  15. [Response of alfalfa seed to stress storage conditions].

    PubMed

    Li, Chunjie; Wang, Yanrong; Zhu, Tingheng; Yu, Ling

    2002-08-01

    The seed germination rate, seed mortality, seedling length, and infection rate of alfalfa (Medicago sativa L. cv. 'Longdong') were measured at constant temperature 20 degrees C every 60 days during one year storage period after inoculated or no inoculated by Fusarium avenaceum under room temperature (RT), 35 degrees C, and 35 degrees C and +10% seed moisture content (SMC) conditions. Field emergence rates of seeds under above treatments were also observed, and seed-borne fungi were detected under the conditions mentioned above and controlled deterioration (CD) as well. The results showed that the percentage of isolated alfalfa seed-borne fungi was increased from 10% under room temperature and 35 degrees C to 29% under 35 degrees C + 10% SMC. Disease resistance was declined, and seed mortality and seedling infection rate under 35 degrees C + 10% SMC were significantly higher than those under room temperature and 35 degrees C respectively (P < 0.05). The seed germination rate and field emergence rate were also decreased significantly (P < 0.05). Seedling shoot and root length under 35 degrees C + 10% SMC were significantly less than those under RT and 35 degrees C respectively (P < 0.05). The percentages of both seed-borne fungi isolated and field emergence were decreased, and that of seedling infection was increased with storage period extending from 60 to 360 days. Compared to no inoculated control, the percentage of seed germination, seedling shoot and root length were decreased, and seed mortality and seedling infection rate were increased after inoculated by F. avenaceum.

  16. Heat Sponge: A Concept for Mass-Efficient Heat Storage

    NASA Technical Reports Server (NTRS)

    Splinter, Scott C.; Blosser, Max L.; Gifford, Andrew R.

    2008-01-01

    The heat sponge is a device for mass-efficient storage of heat. It was developed to be incorporated in the substructure of a re-entry vehicle to reduce thermal- protection-system requirements. The heat sponge consists of a liquid/vapor mixture contained within a number of miniature pressure vessels that can be embedded within a variety of different types of structures. As temperature is increased, pressure in the miniature pressure vessels also increases so that heat absorbed through vaporization of the liquid is spread over a relatively large temperature range. Using water as a working fluid, the heat-storage capacity of the liquid/vapor mixture is many times higher than that of typical structural materials and is well above that of common phase change materials over a temperature range of 200 F to 700 F. The use of pure ammonia as the working fluid provides a range of application between 432 deg R and 730 deg R, or the use of the more practical water-ammonia solution provides a range of application between 432 deg R and 1160 deg R or in between that of water and pure ammonia. Prototype heat sponges were fabricated and characterized. These heat sponges consisted of 1.0-inch-diameter, hollow, stainless-steel spheres with a wall thickness of 0.020 inches which had varying percentages of their interior volumes filled with water and a water-ammonia solution. An apparatus to measure the heat stored in these prototype heat sponges was designed, fabricated, and verified. The heat-storage capacity calculated from measured temperature histories is compared to numerical predictions.

  17. Analysis of Temperature Variability in Medication Storage Compartments in Emergency Transport Helicopters.

    PubMed

    O'Donnell, Margaret A; Whitfield, Justin

    The purpose of this study was to determine whether the temperature in medication storage compartments in air medical helicopters was within United States Pharmacopeia (USP)-defined limits for controlled room temperature. This was a prospective study using data obtained from a continuous temperature monitoring device. A total of 4 monitors were placed within 2 medication storage locations in 2 identical helicopters. The data collection period lasted 2 weeks during the summer and winter seasons. Data retrieved from monitors were compared against USP parameters for proper medication storage. Results documented temperatures outside the acceptable range a majority of the time with temperatures above the high limit during summer and below the low limit during winter. The study determined that compartments used for medication storage frequently fell outside of the range for USP-defined limits for medication storage. Flight programs should monitor storage areas, carefully taking actions to keep medication within defined ranges. Copyright © 2016 Air Medical Journal Associates. Published by Elsevier Inc. All rights reserved.

  18. Correlation between different markers for the assessment of red chilli pepper powders stability during shelf-life.

    PubMed

    Bignardi, Chiara; Cavazza, Antonella; Rinaldi, Massimiliano; Corradini, Claudio

    2016-06-01

    Pungency and red colour of Capsicum powders deteriorate during processing and storage, resulting in a decrease in market value. Two varieties of pepper with different pungencies were monitored for capsaicinoids, colour and furosine. Aliquots were stored at room and at low temperature during one year. At low temperature all indicators were stable in both varieties, while at room temperature, redness and capsacinoids decreased significantly, while furosine increased. High correlation was found between those markers. The more pungent variety exhibited higher stability in terms of all parameters. Differences observed suggest a potential protective effect exerted by capsaicinoids on powder stability. The decrease in capsaicinoids and redness accompanied by furosine increase showed a linkage between those markers never reported before. Considering that capsaicinoids and furosine occurrence have strong impact on the nutritional profile, the findings of this work show relevant changes in the nutritional value of chilli pepper powder after storage.

  19. Reverse Stability Kinetics of Meat Pigment Oxidation in Aqueous Extract from Fresh Beef.

    PubMed

    Frelka, John C; Phinney, David M; Wick, Macdonald P; Heldman, Dennis R

    2017-12-01

    The use of kinetic models is an evolving approach to describing quality changes in foods during processes, including storage. Previous studies indicate that the oxidation rate of myoglobin is accelerated under frozen storage conditions, a phenomenon termed reverse stability. The goal of this study was to develop a model for meat pigment oxidation to incorporate the phenomenon of reverse stability. In this investigation, the model system was an aqueous extract from beef which was stored under a range of temperatures, both unfrozen and frozen. The kinetic analysis showed that in unfrozen solutions, the temperature dependence of oxidation rate followed Arrhenius kinetics. However, under in frozen solutions the rate of oxidation increased with decreasing temperature until reaching a local maximum around -20 °C. The addition of NaCl to the model system increased oxidation rates at all temperatures, even above the initial freezing temperature. This observation suggests that this reaction is dependent on the ionic strength of the solution as well as temperature. The mechanism of this deviant kinetic behavior is not fully understood, but this study shows that the interplay of temperature and composition on the rate of oxidation of meat pigments is complicated and may involve multiple mechanisms. A better understanding of the kinetics of quality loss in a meat system allows for a re-examination of the current recommendations for frozen storage. The deviant kinetic behavior observed in this study indicates that the relationship between quality loss and temperature in a frozen food is not as simple as once thought. Product-specific recommendations could be implemented in the future that would allow for a decrease in energy consumption without a significant loss of quality. © 2017 Institute of Food Technologists®.

  20. Minimizing temperature instability of heat recovery hot water system utilizing optimized thermal energy storage

    NASA Astrophysics Data System (ADS)

    Suamir, I. N.; Sukadana, I. B. P.; Arsana, M. E.

    2018-01-01

    One energy-saving technology that starts gaining attractive for hotel industry application in Indonesia is the utilization of waste heat of a central air conditioning system to heat water for domestic hot water supply system. Implementing the technology for such application at a hotel was found that hot water capacity generated from the heat recovery system could satisfy domestic hot water demand of the hotel. The gas boilers installed in order to back up the system have never been used. The hot water supply, however, was found to be instable with hot water supply temperature fluctuated ranging from 45 °C to 62 °C. The temperature fluctuations reaches 17 °C, which is considered instable and can reduce hot water usage comfort level. This research is aimed to optimize the thermal energy storage in order to minimize the temperature instability of heat recovery hot water supply system. The research is a case study approach based on cooling and hot water demands of a hotel in Jakarta-Indonesia that has applied water cooled chillers with heat recovery systems. The hotel operation with 329 guest rooms and 8 function rooms showed that hot water production in the heat recovery system completed with 5 m3 thermal energy storage (TES) could not hold the hot water supply temperature constantly. The variations of the cooling demand and hot water demands day by day were identified. It was found that there was significant mismatched of available time (hours) between cooling demand which is directly correlated to the hot water production from the heat recovery system and hot water usage. The available TES system could not store heat rejected from the condenser of the chiller during cooling demand peak time between 14.00 and 18.00 hours. The extra heat from the heat recovery system consequently increases the temperature of hot water up to 62 °C. It is about 12 K above 50 °C the requirement hot water temperature of the hotel. In contrast, the TES could not deliver proper temperature of hot water during peak hot water demand and on that time between 06.00 and 10.00 hours, the hotel also experiences a low cooling demand. Subsequently, the temperature of hot water supplied drops down as low as 45 °C. The study was found that optimization on the TES can significantly minimize temperature variation of the hot water supplied to the hotel appliances. A TES of 30 m3 storage capacity is considered the optimum capacity which can reduce the temperature fluctuation from 17 K down to 3 K. The study also found that maintaining the storage temperature relatively lower than the condenser temperature could increase hot water production of the heat recovery system.

  1. Method of encapsulating solid radioactive waste material for storage

    DOEpatents

    Bunnell, Lee Roy; Bates, J. Lambert

    1976-01-01

    High-level radioactive wastes are encapsulated in vitreous carbon for long-term storage by mixing the wastes as finely divided solids with a suitable resin, formed into an appropriate shape and cured. The cured resin is carbonized by heating under a vacuum to form vitreous carbon. The vitreous carbon shapes may be further protected for storage by encasement in a canister containing a low melting temperature matrix material such as aluminum to increase impact resistance and improve heat dissipation.

  2. Study on corrosion of metal materials in nitrate molten salts

    NASA Astrophysics Data System (ADS)

    Zhai, Wei; Yang, Bo; Li, Maodong; Li, Shiping; Xin, Mingliang; Zhang, Shuanghong; Huang, Guojia

    2017-01-01

    High temperature molten salts as a heat transfer heat storage medium has been more widely used in the field of concentrated solar thermal power generation. In the thermal heat storage system, metal material stability and performance at high temperatures are of one major limitation in increasing this operating temperature. In this paper, study on corrosion of 321H, 304, 316L, P91 metal materials in modified solar two molten salts. The corrosion kinetics of 304, 316L, 321H, P91 metal material in the modified solar two molten salts at 450°C, 500°C is also investigated. Under the same condition it was found that 304, 321H corroded at a rate of 40% less than P91. Spallation of corrosion products was observed on P91 steel, while no obvious observed on other kinds of stainless steel. Corrosion rates of 304, 321H, and 316L slowly increased with temperature. Oxidation mechanisms little varied with temperature. Corrosion products of metal materials observed at 450°C, 500°C were primarily Fe oxide and Fe, Cr oxide.

  3. Influence of acidification, pasteurization, centrifugation and storage time and temperature on watermelon juice quality.

    PubMed

    Tarazona-Díaz, Martha Patricia; Aguayo, Encarna

    2013-12-01

    Watermelon juice has gained increasing popularity among consumers as a rich natural source of functional compounds such as lycopene and citrulline. However, the final quality of the juice depends significantly on its acidification, pasteurization, centrifugation and storage time and temperature. In this study, these characteristics were assessed in watermelon juice pasteurized at 87.7 °C for 20 s and stored for up to 30 days at 4 or 8 °C. The acidifier citric acid provided an adequate sensory quality, similar to natural watermelon juice. Centrifugation and pasteurization significantly reduced the red color, bioactive compounds (lycopene, antioxidant capacity and total polyphenols) and sensory quality of the juice, particularly when the storage time was extended and a temperature of 8 °C was used (P ≤ 0.05). All treated juices were microbiologically safe for up to 30 days when stored at 4 or 8 °C. In terms of sensory acceptability, only non-centrifuged juices stored for up to 20 days at 4 °C remained above the commercial limit. The present results suggest that using a non-centrifugation process and a storage temperature of 4 °C yields a watermelon juice that better retains its sensory and functional qualities. © 2013 Society of Chemical Industry.

  4. High-Temperature Phase Change Materials (PCM) Candidates for Thermal Energy Storage (TES) Applications

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Gomez, J. C.

    2011-09-01

    It is clearly understood that lower overall costs are a key factor to make renewable energy technologies competitive with traditional energy sources. Energy storage technology is one path to increase the value and reduce the cost of all renewable energy supplies. Concentrating solar power (CSP) technologies have the ability to dispatch electrical output to match peak demand periods by employing thermal energy storage (TES). Energy storage technologies require efficient materials with high energy density. Latent heat TES systems using phase change material (PCM) are useful because of their ability to charge and discharge a large amount of heat from amore » small mass at constant temperature during a phase transformation like melting-solidification. PCM technology relies on the energy absorption/liberation of the latent heat during a physical transformation. The main objective of this report is to provide an assessment of molten salts and metallic alloys proposed as candidate PCMs for TES applications, particularly in solar parabolic trough electrical power plants at a temperature range from 300..deg..C to 500..deg.. C. The physical properties most relevant for PCMs service were reviewed from the candidate selection list. Some of the PCM candidates were characterized for: chemical stability with some container materials; phase change transformation temperatures; and latent heats.« less

  5. High Energy Storage Density and Impedance Response of PLZT2/95/5 Antiferroelectric Ceramics.

    PubMed

    Li, Bi; Liu, Qiuxiang; Tang, Xingui; Zhang, Tianfu; Jiang, Yanping; Li, Wenhua; Luo, Jie

    2017-02-08

    (Pb 0.97 La 0.02 )(Zr 0.95 Ti 0.05 )O₃ (PLZT2/95/5) ceramics were successfully prepared via a solid-state reaction route. The dielectric properties were investigated in the temperature region of 26-650 °C. The dielectric diffuse anomaly in the dielectric relaxation was found in the high temperature region of 600-650 °C with increasing the measuring frequency, which was related to the dynamic thermal process of ionized oxygen vacancies generated in the high temperature. Two phase transition points were detected during heating, which were found to coexist from 150 to 200 °C. Electric field induced ferroelectric to antiferroelectric phase transition behavior of the (Pb 0.97 La 0.02 )(Zr 0.95 Ti 0.05 )O₃ ceramics was investigated in this work with an emphasis on energy storage properties. A recoverable energy-storage density of 0.83 J/cm³ and efficiency of 70% was obtained in (Pb 0.97 La 0.02 )(Zr 0.95 Ti 0.05 )O₃ ceramics at 55 kV/cm. Based on these results, (Pb 0.97 La 0.02 )(Zr 0.95 Ti 0.05 )O₃ ceramics with a large recoverable energy-storage density could be a potential candidate for the applications in high energy-storage density ceramic capacitors.

  6. Characteristics of phase-change materials containing oxide nano-additives for thermal storage.

    PubMed

    Teng, Tun-Ping; Yu, Chao-Chieh

    2012-11-06

    In this study, the authors report the production of nanocomposite-enhanced phase-change materials (NEPCMs) using the direct-synthesis method by mixing paraffin with alumina (Al2O3), titania (TiO2), silica (SiO2), and zinc oxide (ZnO) as the experimental samples. Al2O3, TiO2, SiO2, and ZnO were dispersed into three concentrations of 1.0, 2.0, and 3.0 wt.%. Through heat conduction and differential scanning calorimeter experiments to evaluate the effects of varying concentrations of the nano-additives on the heat conduction performance and thermal storage characteristics of NEPCMs, their feasibility for use in thermal storage was determined. The experimental results demonstrate that TiO2 is more effective than the other additives in enhancing both the heat conduction and thermal storage performance of paraffin for most of the experimental parameters. Furthermore, TiO2 reduces the melting onset temperature and increases the solidification onset temperature of paraffin. This allows the phase-change heat to be applicable to a wider temperature range, and the highest decreased ratio of phase-change heat is only 0.46%, compared to that of paraffin. Therefore, this study demonstrates that TiO2, added to paraffin to form NEPCMs, has significant potential for enhancing the thermal storage characteristics of paraffin.

  7. Phase-change wallboard and mechanical night ventilation in commercial buildings: Potential for HVAC system downsizing

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Stetiu, C.; Feustel, H.E.

    1998-07-01

    As thermal storage media, phase-change materials (PCMs) such as paraffin, eutectic salts, etc. offer an order-of-magnitude increase in thermal storage capacity, and their discharge is almost isothermal. By embedding PCMs in dypsum board, plaster, or other wall-covering materials, the building structure acquires latent storage properties. Structural elements containing PCMs can store large amounts of energy while maintaining the indoor temperature within a relatively narrow range. As heat storage takes place inside the building where the loads occur, rather than at a central exterior location, the internal loads are removed without the need for additional transport energy. Distributed latent storage canmore » thus be used to reduce the peak power demand of a building, downsize the cooling system, and/or switch to low-energy cooling sources. The authors used RADCOOL, a thermal building simulation program based on the finite difference approach, to numerically evaluate the thermal performance of PCM wallboard coupled with mechanical night ventilation in office buildings offers the opportunity for system downsizing in climates where the outside air temperature drops below 18 C at night. In climates where the outside air temperature remains above 19 C at night, the use of PCM wallboard should be coupled with discharge mechanisms other than mechanical night ventilation with outside air.« less

  8. [The dynamics of chromosomal instability of welsh onion (Allium fistulosum L.): the influence of seed storage temperature].

    PubMed

    Lazarenko, L M; Bezrukov, V F

    2008-01-01

    The age-related dynamics of chromosomal instability and germination capacity of welsh onion (Allium fistulosum L.) seeds have been studied under two different storage temperatures during six years after harvesting. Seeds that were kept at the room temperature (14-28 degrees C) during 6 years of storage have lost their germination capacity. The frequencies of aberrant anaphases grew from 2% on the first month of storage up to 80% on the 75th month of storage. The germination capacity of seeds kept at the lower temperature (4-9 degrees C) was 73-77% on the 6th year of storage and the frequency of aberrant anaphases remained within the limits of 2-4%. Thus, storage of welsh onion seeds during 6 years at the lower temperature allows to retain germination capacity and restrains the augmentation of chromosomal instability in root meristem cells of seedlings during this period.

  9. 16 CFR 305.3 - Description of covered products.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... refrigerator means a cabinet designed for the refrigerated storage of food at temperatures above 32 °F and... a separate low temperature compartment designed for the freezing and storage of food at temperatures... with at least one of the compartments designed for the refrigerated storage of food at temperatures...

  10. 16 CFR 305.3 - Description of covered products.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... refrigerator means a cabinet designed for the refrigerated storage of food at temperatures above 32 °F and... a separate low temperature compartment designed for the freezing and storage of food at temperatures... with at least one of the compartments designed for the refrigerated storage of food at temperatures...

  11. Purification and autolysis of the ficin isoforms from fig (Ficus carica cv. Sabz) latex

    PubMed Central

    Zare, Hamid; Moosavi-Movahedi, Ali Akbar; Salami, Maryam; Mirzaei, Morteza; Saboury, Ali Akbar; Sheibani, Nader

    2013-01-01

    Ficin (EC 3.4.22.3), a cysteine endoproteolytic protease in fig trees’ latex, has multiple isoforms. Until now, no data on autolysis of individual ficins (ficin isoforms) are available. Following purification, ficins’ autolysis was determined by HPLC chromatogram changes and ultrafiltrations at different temperatures and storage times. These results showed that the number of HPLC peaks in latex proteins purification of Ficus carica cv. Sabz varied from previous fig varieties or cultivars. Proteolytic activity of ficins was inhibited by specific cysteine protease inhibitors, confirming the participation of the cysteine residue in the active site. The zeta potential of the first two eluted peaks (I and II) was negative, while that of other peaks were positive. All ficins were susceptible to autolysis when stored at high temperatures. In contrast, only the last two ficins (B, C) were prone to autolysis at cold temperature after long storage period. The rate of degradation of the ficins was significantly increased with the increased storage time. The ficin (A) related to peak (III) had the highest and the lowest surface hydrophobic patches and ratio of autolytic to proteolytic activity, respectively. PMID:23312458

  12. Time-Temperature Profiling of United Kingdom Consumers' Domestic Refrigerators.

    PubMed

    Evans, Ellen W; Redmond, Elizabeth C

    2016-12-01

    Increased consumer demand for convenience and ready-to-eat food, along with changes to consumer food purchase and storage practices, have resulted in an increased reliance on refrigeration to maximize food safety. Previous research suggests that many domestic refrigerators operate at temperatures exceeding recommendations; however, the results of several studies were determined by means of one temperature data point, which, given temperature fluctuation, may not be a true indicator of actual continual operating temperatures. Data detailing actual operating temperatures and the effects of consumer practices on temperatures are limited. This study has collated the time-temperature profiles of domestic refrigerators in consumer kitchens (n = 43) over 6.5 days with concurrent self-reported refrigerator usage. Overall, the findings established a significant difference (P < 0.05) between one-off temperature (the recording of one temperature data point) and mean operating temperature. No refrigerator operated at ≤5.0°C for the entire duration of the study. Mean temperatures exceeding 5.0°C were recorded in the majority (91%) of refrigerators. No significant associations or differences were determined for temperature profiles and demographics, including household size, or refrigerator characteristics (age, type, loading, and location). A positive correlation (P < 0.05) between room temperature and refrigerator temperature was determined. Reported door opening frequency correlated with temperature fluctuation (P < 0.05). Thermometer usage was determined to be infrequent. Cumulatively, research findings have established that the majority of domestic refrigerators in consumer homes operate at potentially unsafe temperatures and that this is influenced by consumer usage. The findings from this study may be utilized to inform the development of shelf-life testing based on realistic domestic storage conditions. Furthermore, the data can inform the development of future educational interventions to increase safe domestic refrigeration practices.

  13. Quality and storage stability of extruded puffed corn-fish snacks during 6-month storage at ambient temperature.

    PubMed

    Shaviklo, Gholam Reza; Thorkelsson, Gudjon; Rafipour, Fereidon; Sigurgisladottir, Sjofn

    2011-03-30

    Cereal-based snacks are usually low in protein and other nutrients. Increased health awareness of consumers has led the food industry to develop fortified snacks with functional ingredients. Three types of extruded corn-fish snacks, containing 150 g kg(-1) carp mince and 150 g kg(-1) trout mince, 30 g kg(-1) freeze-dried saithe protein and a regular corn snack (control). were produced to study quality changes and storage stability of the products during 6-month storage at 27±2 °C. All products had the same level of water activity and proximate composition except for protein. Fortified snacks had a protein content of 93-98 g kg(-1) , compared with 65 g kg(-1) in the control. A significant increase was observed for peroxide value during storage (0.0 to 2.8 meq kg(-1)). Scores for attributes describing oxidation and off odors and flavors increased after 5-6 months' storage but attributes describing puffed corn snack odor and flavor did not change during storage of any of the products. Extrusion of corn grits with fish flesh/fish protein can be used to produce high-protein products that would be an option to provide nutrient snacks for consumers and to increase fish consumption. Copyright © 2011 Society of Chemical Industry.

  14. High temperature metal hydrides as heat storage materials for solar and related applications.

    PubMed

    Felderhoff, Michael; Bogdanović, Borislav

    2009-01-01

    For the continuous production of electricity with solar heat power plants the storage of heat at a temperature level around 400 degrees C is essential. High temperature metal hydrides offer high heat storage capacities around this temperature. Based on Mg-compounds, these hydrides are in principle low-cost materials with excellent cycling stability. Relevant properties of these hydrides and their possible applications as heat storage materials are described.

  15. High Temperature Metal Hydrides as Heat Storage Materials for Solar and Related Applications

    PubMed Central

    Felderhoff, Michael; Bogdanović, Borislav

    2009-01-01

    For the continuous production of electricity with solar heat power plants the storage of heat at a temperature level around 400 °C is essential. High temperature metal hydrides offer high heat storage capacities around this temperature. Based on Mg-compounds, these hydrides are in principle low-cost materials with excellent cycling stability. Relevant properties of these hydrides and their possible applications as heat storage materials are described. PMID:19333448

  16. Production of salted and pressed anchovies (stolephorus sp.) and it's quality evaluation during storage.

    PubMed

    Siriskar, Dipty A; Khedkar, Gulab Dattarao; Lior, David

    2013-12-01

    Present study deals with the production of low cost salted and dried product from anchovy fish (Stolephorus spp.) using traditional technology application of different inhibitory factors to achieve a reasonable shelf life at ambient tropical temperature. Microbial safety, nutrient retention and product quality in terms of flavor and texture after rehydration have been tested. This product has been subjected to storage studies, with respect to product quality and microbial status. It can be kept well for a period of 5 weeks. The quality changes during storage are; decrease in sensory scores for the overall acceptance and also for the individual attributes. Increase in nitrogenous components such as Total Volatile Bases Nitrogen (TVB-N), Tri Methyl Amine Nitrogen (TMA-N) and Alpha Amino Nitrogen (AAN) was obtained with the advancement of the storage period. The lipid quality of the fish showed gradual increase in Preoxide Value (PV) and Free Fatty Acid (FFA) accompanied by decrease in flavor and odor scores. Activity of microorganisms showed a marginal increase with increase in storage period.

  17. Changes in inulin and soluble sugar concentration in artichokes (Cynara scolymus L.) during storage.

    PubMed

    Leroy, Gaëlle; Grongnet, Jean François; Mabeau, Serge; Corre, Daniel Le; Baty-Julien, Céline

    2010-05-01

    The artichoke (Cynara scolymus L.) accumulates about 50-70 g kg(-1) of its fresh weight as inulin-type fructan. Inulin fermentation increases gas production and thereby provokes intestinal discomfort in some people. The present research focuses on the changes in carbohydrate composition occurring in artichoke heads during storage under different conditions (18 degrees C, 4 degrees C and 4 degrees C under polypropylene film packing). Carbohydrate content and composition were determined by anion-exchange high-performance liquid chromatography with pulsed amperometric detection. Storage time caused a decrease in inulin content and an average degree of polymerization, accompanied by an increase of free fructose and sucrose due to depolymerization of inulin. Higher-temperature storage and storage without packing induce strong carbohydrate changes. Thereby, eating stored artichoke leads to consumption of an inulin quantity that does not provoke unwanted symptoms related to gas production but sufficient to have a prebiotic effect.

  18. Thermophysical and Mechanical Properties of Hardened Cement Paste with Microencapsulated Phase Change Materials for Energy Storage.

    PubMed

    Cui, Hongzhi; Liao, Wenyu; Memon, Shazim Ali; Dong, Biqin; Tang, Waiching

    2014-12-16

    In this research, structural-functional integrated cement-based materials were prepared by employing cement paste and a microencapsulated phase change material (MPCM) manufactured using urea-formaldehyde resin as the shell and paraffin as the core material. The encapsulation ratio of the MPCM could reach up to 91.21 wt%. Thermal energy storage cement pastes (TESCPs) incorporated with different MPCM contents (5%, 10%, 15%, 20% and 25% by weight of cement) were developed, and their thermal and mechanical properties were studied. The results showed that the total energy storage capacity of the hardened cement specimens with MPCM increased by up to 3.9-times compared with that of the control cement paste. The thermal conductivity at different temperature levels (35-36 °C, 55-56 °C and 72-74 °C) decreased with the increase of MPCM content, and the decrease was the highest when the temperature level was 55-56 °C. Moreover, the compressive strength, flexural strength and density of hardened cement paste decreased with the increase in MPCM content linearly. Among the evaluated properties, the compressive strength of TESCPs had a larger and faster degradation with the increase of MPCM content.

  19. The effects of temperature on the crystalline properties and resistant starch during storage of white bread.

    PubMed

    Sullivan, William R; Hughes, Jeff G; Cockman, Russell W; Small, Darryl M

    2017-08-01

    Resistant starch (RS) can form during storage of foods, thereby bestowing a variety of potential health benefits. The purpose of the current study has been to determine the influence of storage temperature and time on the crystallinity and RS content of bread. Loaves of white bread were baked and stored at refrigeration, frozen and room temperatures with analysis over a period of zero to seven days. RS determination and X-ray diffraction (XRD) were used to evaluate the influence of storage temperature and time on total crystallinity and RS content. The rate of starch recrystallisation was affected by storage temperature and time, where refrigeration temperatures accelerated RS formation and total crystallinity more than storage time at both frozen and room temperature. A strong statistical model has been established between RS formation in bread and XRD patterns, having a 96.7% fit indicating the potential of XRD to measure RS concentrations. Copyright © 2017 Elsevier Ltd. All rights reserved.

  20. Long-Term Stability of Mold Compounds and the Influence on Semiconductor Device Reliability

    NASA Astrophysics Data System (ADS)

    Mahler, Joachim; Mengel, Manfred

    2012-07-01

    Lifetimes of semiconductor devices are specified according to the products and their applications to ensure safe operation, for instance as part of an automobile product. The long-term stability of the device is strongly dependent on the chip encapsulation and its adhesion to the chip and substrate. Molded silicon strips that act as a model system for molded chips inside semiconductor devices were investigated. Four commercially available mold compounds were applied on silicon strips and stored over 5 years at room temperature (RT), and changes in the thermomechanical behavior were analyzed. After storage, all molded strips exhibited warpage reduction in the range of 11% to 14% at RT with respect to the initial warpage. The temperatures for the stress-free state also changed during storage and were located between 228°C and 235°C for each mold. Additional stress applied to the stored modules, by temperature cycling as well as high-temperature storage, increased the warpage of the molded silicon samples. For further interpretation of measured results, finite-element method calculations were performed.

  1. Folate content in tomato ( Lycopersicon esculentum ). influence of cultivar, ripeness, year of harvest, and pasteurization and storage temperatures.

    PubMed

    Iniesta, M Dolores; Pérez-Conesa, Darío; García-Alonso, Javier; Ros, Gaspar; Periago, M Jesús

    2009-06-10

    The effects of cultivar, on-vine ripening, and year of harvest on the folate content of raw tomatoes were studied. Folate content in hot-break tomato puree (HTP) subjected to pasteurization at different temperatures and its evolution during the shelf life of tomato juice were also investigated. 5-Methyltetrahydrofolate (5-CH(3)-H(4)-folate) was the only folate compound identified in raw tomatoes and HTP, but tetrahydrofolate (H(4)-folate) was 10% of the folate detected in tomato juice. The content of folates in raw tomatoes ranged from 4.1 to 35.3 microg/100 g of fresh weight and was highly influenced by all of the factors studied. No clear trend of folate content with ripening stage was observed. The extractability of 5-CH(3)-H(4)-folate from HTP increased significantly after pasteurization at 98 degrees C for 40 s, but higher temperatures decreased its content. Tomato juice showed folate losses during storage independent of the storage temperature. Folate losses were higher when tomato juice was packed in glass bottles than in Tetra Pak.

  2. Storage of Fruits and Vegetables in Refrigerator Increases their Phenolic Acids but Decreases the Total Phenolics, Anthocyanins and Vitamin C with Subsequent Loss of their Antioxidant Capacity

    PubMed Central

    Patel, Nilesh J.; Talati, Jayant G.

    2017-01-01

    It is of paramount importance for consumers, scientists and industrialists to understand how low-temperature storage of food items affects their bioactive compounds and properties. This study evaluated the effects of cold storage on total phenolics (TP), phenolic acids profile (PA), total anthocyanins (TA), total ascorbic acid (Vit. C) and antioxidant activity (AA) of 19 fruits and vegetables, collected from local Indian markets and stored in refrigerator (4 °C) during 15 days. Content of TP was highest in dill and amaranth and decreased (up to 29.67%) with storage. Leafy vegetables (amaranth, dill, onion, fenugreek and spinach) contained higher amounts of the 12 PA revealed by UPLC-UV; ellagic, gallic, sinapic and vanillic acids levels were the highest; chlorogenic acid (ρ = 0.423), syringic acid (ρ = 0.403) and sinapic acid (ρ = 0.452) mostly correlated with TP; and the PA increased during storage. Highest contents of Vit C estimated by AOAC, DCPIP and DNP methods were found in amaranth, dill and pomegranate, and decreased with storage. Pomegranate showed highest TA levels and low-temperature storage did not significantly increase TA, which was the largest contributor of TP in fruits and vegetables (ρ = 0.661). Storage induced a drastic decrease of AA, which mostly correlated with TP (ρ = 0.808, 0.690 and 0.458 for DPPH, ABTS and FRAP assays, respectively). Spearman’s correlation confirmed by principal component analysis demonstrated that dill, pomegranate and amaranth had the highest overall antioxidant capacity, whereas orange juice and carrot showed the lowest. The results provide support for a key-role of TP, followed by Vit. C and TA in antioxidant capacity of fruits and vegetables, which could be interesting dietary sources of natural antioxidants for prevention of diseases caused by oxidative stress. PMID:28737734

  3. Storage of Fruits and Vegetables in Refrigerator Increases their Phenolic Acids but Decreases the Total Phenolics, Anthocyanins and Vitamin C with Subsequent Loss of their Antioxidant Capacity.

    PubMed

    Galani, Joseph H Y; Patel, Jalpesh S; Patel, Nilesh J; Talati, Jayant G

    2017-07-24

    It is of paramount importance for consumers, scientists and industrialists to understand how low-temperature storage of food items affects their bioactive compounds and properties. This study evaluated the effects of cold storage on total phenolics (TP), phenolic acids profile (PA), total anthocyanins (TA), total ascorbic acid (Vit. C) and antioxidant activity (AA) of 19 fruits and vegetables, collected from local Indian markets and stored in refrigerator (4 °C) during 15 days. Content of TP was highest in dill and amaranth and decreased (up to 29.67%) with storage. Leafy vegetables (amaranth, dill, onion, fenugreek and spinach) contained higher amounts of the 12 PA revealed by UPLC-UV; ellagic, gallic, sinapic and vanillic acids levels were the highest; chlorogenic acid (ρ = 0.423), syringic acid (ρ = 0.403) and sinapic acid (ρ = 0.452) mostly correlated with TP; and the PA increased during storage. Highest contents of Vit C estimated by AOAC, DCPIP and DNP methods were found in amaranth, dill and pomegranate, and decreased with storage. Pomegranate showed highest TA levels and low-temperature storage did not significantly increase TA, which was the largest contributor of TP in fruits and vegetables (ρ = 0.661). Storage induced a drastic decrease of AA, which mostly correlated with TP (ρ = 0.808, 0.690 and 0.458 for DPPH, ABTS and FRAP assays, respectively). Spearman's correlation confirmed by principal component analysis demonstrated that dill, pomegranate and amaranth had the highest overall antioxidant capacity, whereas orange juice and carrot showed the lowest. The results provide support for a key-role of TP, followed by Vit. C and TA in antioxidant capacity of fruits and vegetables, which could be interesting dietary sources of natural antioxidants for prevention of diseases caused by oxidative stress.

  4. Polyaniline-polypyrrole composites with enhanced hydrogen storage capacities.

    PubMed

    Attia, Nour F; Geckeler, Kurt E

    2013-06-13

    A facile method for the synthesis of polyaniline-polypyrrole composite materials with network morphology is developed based on polyaniline nanofibers covered by a thin layer of polypyrrole via vapor phase polymerization. The hydrogen storage capacity of the composites is evaluated at room temperature exhibits a twofold increase in hydrogen storage capacity. The HCl-doped polyaniline nanofibers exhibit a storage capacity of 0.46 wt%, whereas the polyaniline-polypyrrole composites could store 0.91 wt% of hydrogen gas. In addition, the effect of the dopant type, counteranion size, and the doping with palladium nanoparticles on the storage properties are also investigated. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  5. Development of shelf stable pork sausages using hurdle technology and their quality at ambient temperature (37±1°C) storage.

    PubMed

    Thomas, R; Anjaneyulu, A S R; Kondaiah, N

    2008-05-01

    Shelf stable pork sausages were developed using hurdle technology and their quality was evaluated during ambient temperature (37±1°C) storage. Hurdles incorporated were low pH, low water activity, vacuum packaging and post package reheating. Dipping in potassium sorbate solution prior to vacuum packaging was also studied. Reheating increased the pH of the sausages by 0.17units as against 0.11units in controls. Incorporation of hurdles significantly decreased emulsion stability, cooking yield, moisture and fat percent, yellowness and hardness, while increasing the protein percent and redness. Hurdle treatment reduced quality deterioration during storage as indicated by pH, TBARS and tyrosine values. About 1 log reduction in total plate count was observed with the different hurdles as were reductions in the coliform, anaerobic, lactobacilli and Staphylococcus aureus counts. pH, a(w) and reheating hurdles inhibited yeast and mold growth up to day 3, while additional dipping in 1% potassium sorbate solution inhibited their growth throughout the 9 days storage. Despite low initial sensory appeal, the hurdle treated sausages had an overall acceptability in the range 'very good' to 'good' up to day 6.

  6. Comparability of the effect of storage time and temperature on serum anti-Müllerian hormone measurement between original and modified enzyme-linked immunosorbent assay.

    PubMed

    Yue, Chao-Yan; Ying, Chun-Mei

    2017-01-01

    To explore the effect of modified enzyme-linked immunosorbent assay on the AMH results is increased or decreased, and to investigate the effect of storage time and temperature on AMH measurements with and without sample premixing assay buffer using the Kangrun ELISA method. Serum AMH concentration were measured by ELISA, consistency between two kits, and comparability between original and the modified assay under different stored conditions were analyzed by Passing-Bablok regression analysis and Bland-Altman bias evaluation. There was a strong consistency between AMH concentrations measured in Kangrun ELISA and Ansh Labs ultra-sensitive AMH ELISA. Pre-mixing serum specimens with assay buffer gave consistent results compared with original assay. Modified protocol can reduce the amplitude of increase affected by sample aged and give the most consistent results regardless of storage conditions. Pre-mixing protocol did not influence the results of fresh serum or frozen serum incubation <3days at 4°C and -80°C, but when specimens detected after collection and stored in other storage conditions, should be pre-mixed with assay buffer to insure its accuracy. Copyright © 2016 Elsevier B.V. All rights reserved.

  7. Permeation of Military Fuels Through Nitrile-Coated Fabrics Used for Collapsible Fuel Storage Containers

    DTIC Science & Technology

    2014-03-01

    resistance; while decreasing the amount of acrylonitrile content improves low-temperature flexibility, but increases transport rates of military fuels through...tanks do suffer from an increase in total weight and reduced flexibility, which may influence storage, transportation , and setup of the containers...exterior surfaces. The transport of the fuel can be described by Fick’s first law (11): c J=-P x   (1) Where J is the fuel vapor flux, P is

  8. Results for the Aboveground Configuration of the Boiling Water Reactor Dry Cask Simulator

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Durbin, Samuel G.; Lindgren, Eric Richard

    The thermal performance of commercial nuclear spent fuel dry storage casks are evaluated through detailed numerical analysis. These modeling efforts are completed by the vendor to demonstrate performance and regulatory compliance. The calculations are then independently verified by the Nuclear Regulatory Commission (NRC). Carefully measured data sets generated from testing of full sized casks or smaller cask analogs are widely recognized as vital for validating these models. Recent advances in dry storage cask designs have significantly increased the maximum thermal load allowed in a cask in part by increasing the efficiency of internal conduction pathways and also by increasing themore » internal convection through greater canister helium pressure. These same canistered cask systems rely on ventilation between the canister and the overpack to convect heat away from the canister to the environment for both above and belowground configurations. While several testing programs have been previously conducted, these earlier validation attempts did not capture the effects of elevated helium pressures or accurately portray the external convection of aboveground and belowground canistered dry cask systems. The purpose of the current investigation was to produce data sets that can be used to test the validity of the assumptions associated with the calculations used to determine steady-state cladding temperatures in modern dry casks that utilize elevated helium pressure in the sealed canister in an aboveground configuration. An existing electrically heated but otherwise prototypic BWR Incoloy-clad test assembly was deployed inside of a representative storage basket and cylindrical pressure vessel that represents a vertical canister system. The symmetric single assembly geometry with well-controlled boundary conditions simplifies interpretation of results. The arrangement of ducting was used to mimic conditions for an aboveground storage configuration in a vertical, dry cask systems with canisters. Transverse and axial temperature profiles were measured for a wide range of decay power and helium cask pressures. Of particular interest was the evaluation of the effect of increased helium pressure on peak cladding temperatures (PCTs) for identical thermal loads. All steady state peak temperatures and induced flow rates increased with increasing assembly power. Peak cladding temperatures decreased with increasing internal helium pressure for a given assembly power, indicating increased internal convection. In addition, the location of the PCT moved from near the top of the assembly to ~1/3 the height of the assembly for the highest (8 bar absolute) to the lowest (0 bar absolute) pressure studied, respectively. This shift in PCT location is consistent with the varying contribution of convective heat transfer proportional with of internal helium pressure.« less

  9. Small scale changes of geochemistry and flow field due to transient heat storage in aquifers

    NASA Astrophysics Data System (ADS)

    Bauer, S.; Boockmeyer, A.; Li, D.; Beyer, C.

    2013-12-01

    Heat exchangers in the subsurface are increasingly installed for transient heat storage due to the need of heating or cooling of buildings as well as the interim storage of heat to compensate for the temporally fluctuating energy production by wind or solar energy. For heat storage to be efficient, high temperatures must be achieved in the subsurface. Significant temporal changes of the soil and groundwater temperatures however effect both the local flow field by temperature dependent fluid parameters as well as reactive mass transport through temperature dependent diffusion coefficients, geochemical reaction rates and mineral equilibria. As the use of heat storage will be concentrated in urban areas, the use of the subsurface for (drinking) water supply and heat storage will typically coincide and a reliable prognosis of the processes occurring is needed. In the present work, the effects of a temporal variation of the groundwater temperature, as induced by a local heat exchanger introduced into a groundwater aquifer, are studied. For this purpose, the coupled non-isothermal groundwater flow, heat transport and reactive mass transport is simulated in the near filed of such a heat exchanger. By explicitly discretizing and incorporating the borehole, the borehole cementation and the heat exchanger tubes, a realistic geometrical and process representation is obtained. The numerical simulation code OpenGeoSys is used in this work, which incorporates the required processes of coupled groundwater flow, heat and mass transport as well as temperature dependent geochemistry. Due to the use of a Finite Element Method, a close representation of the geometric effects can be achieved. Synthetic scenario simulations for typical settings of salt water formations in northern Germany are used to investigate the geochemical effects arising from a high temperature heat storage by quantifying changes in groundwater chemistry and overall reaction rates. This work presents the simulation approach used and results obtained for the synthetic scenarios. The model simulations show that locally in the direct vicinity of the borehole heat exchanger the flow field is changed, causing a ground water convergence and thus a mixing of water in the case of high temperatures. Also, geochemical reactions are induced due to shifting of temperature dependent mineral equilibria. Due to the moving groundwater, the changes are not reversible, and small impacts remain downstream of the borehole heat exchanger. However, the changes depend strongly on the mineral composition of the formation and the formation water present.

  10. 14 CFR 23.1353 - Storage battery design and installation.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... take appropriate load shedding action. [Doc. No. 4080, 29 FR 17955, Dec. 18, 1964; 30 FR 258, Jan. 9... any probable charging and discharging condition. No uncontrolled increase in cell temperature may... temperatures and pressures presents no problem. (d) No explosive or toxic gases emitted by any battery in...

  11. Heat storage in forest biomass improves energy balance closure

    NASA Astrophysics Data System (ADS)

    Lindroth, A.; Mölder, M.; Lagergren, F.

    2010-01-01

    Temperature measurements in trunks and branches in a mature ca. 100 years-old mixed pine and spruce forest in central Sweden were used to estimate the heat storage in the tree biomass. The estimated heat flux in the sample trees and data on biomass distributions were used to scale up to stand level biomass heat fluxes. The rate of change of sensible and latent heat storage in the air layer below the level of the flux measurements was estimated from air temperature and humidity profile measurements and soil heat flux was estimated from heat flux plates and soil temperature measurements. The fluxes of sensible and latent heat from the forest were measured with an eddy covariance system in a tower. The analysis was made for a two-month period in summer of 1995. The tree biomass heat flux was the largest of the estimated storage components and varied between 40 and -35 W m-2 on summer days with nice weather. Averaged over two months the diurnal maximum of total heat storage was 45 W m-2 and the minimum was -35 W m-2. The soil heat flux and the sensible heat storage in air were out of phase with the biomass flux and they reached maximum values that were about 75% of the maximum of the tree biomass heat storage. The energy balance closure improved significantly when the total heat storage was added to the turbulent fluxes. The slope of a regression line with sum of fluxes and storage as independent and net radiation as dependent variable, increased from 0.86 to 0.95 for half-hourly data and the scatter was also reduced. The most significant finding was, however, that during nights with strongly stable conditions when the sensible heat flux dropped to nearly zero, the total storage matched the net radiation very well. Another interesting result was that the mean energy imbalance started to increase when the Richardson number became more negative than ca. -0.1. In fact, the largest energy deficit occurred at maximum instability. Our conclusion is that eddy covariance measurements can function well during stable conditions but that the functioning under strong instabilities might be a so far unforeseen problem.

  12. Heat storage in forest biomass significantly improves energy balance closure particularly during stable conditions

    NASA Astrophysics Data System (ADS)

    Lindroth, A.; Mölder, M.; Lagergren, F.

    2009-08-01

    Temperature measurements in trunks and branches in a mature ca. 100 years-old mixed pine and spruce forest in central Sweden were used to estimate the heat storage in the tree biomass. The estimated heat flux in the sample trees and data on biomass distributions were used to scale up to stand level biomass heat fluxes. The rate of change of sensible and latent heat storage in the air layer below the level of the flux measurements was estimated from air temperature and humidity profile measurements and soil heat flux was estimated from heat flux plates and soil temperature measurements. The fluxes of sensible and latent heat from the forest were measured with an eddy covariance system in a tower. The analysis was made for a two-month period in summer of 1995. The tree biomass heat flux was the largest of the estimated storage components and varied between 40 and -35 W m-2 on summer days with nice weather. Averaged over two months the diurnal maximum of total heat storage was 45 W m-2 and the minimum was -35 W m-2. The soil heat flux and the sensible heat storage in air were out of phase with the biomass flux and they reached maximum values that were about 75% of the maximum of the tree biomass heat storage. The energy balance closure improved significantly when the total heat storage was added to the turbulent fluxes. The slope of a regression line with sum of fluxes and storage as independent and net radiation as dependent variable, increased from 0.86 to 0.95 for half-hourly data and the scatter was also reduced. The most significant finding was, however, that during nights with strongly stable conditions when the sensible heat flux dropped to nearly zero, the total storage matched the net radiation nearly perfectly. Another interesting result was that the mean energy imbalance started to increase when the Richardson number became more negative than ca. -0.1. In fact, the largest energy deficit occurred at maximum instability. Our conclusion is that eddy covariance measurements can function well during stable conditions but that the functioning under strong instabilities might be a so far unforeseen problem.

  13. Some rheological properties of sodium caseinate-starch gels.

    PubMed

    Bertolini, Andrea C; Creamer, Lawrence K; Eppink, Mieke; Boland, Mike

    2005-03-23

    The influence of sodium caseinate on the thermal and rheological properties of starch gels at different concentrations and from different botanical sources was evaluated. In sodium caseinate-starch gels, for all starches with the exception of potato starch, the sodium caseinate promoted an increase in the storage modulus and in the viscosity of the composite gel when compared with starch gels. The addition of sodium caseinate resulted in an increase in the onset temperature, the gelatinization temperature, and the end temperature, and there was a significant interaction between starch and sodium caseinate for the onset temperature, the peak temperature, and the end temperature. Microscopy results suggested that sodium caseinate promoted an increase in the homogeneity in the matrix of cereal starch gels.

  14. Influence of Beaver Dams on Channel Complexity, Hydrology, and Temperature Regime in a Mountainous Stream

    NASA Astrophysics Data System (ADS)

    Majerova, M.; Neilson, B. T.; Schmadel, N. M.; Wheaton, J. M.; Snow, C. J.

    2013-12-01

    Beaver dams and beaver activity affect hydrologic processes, sediment transport, channel complexity and water quality of streams. Beaver ponds, which form behind beaver dams, increase in-channel water storage affecting the timing and volume of flow and resulting in the attenuation and flattening of the hydrograph. Channel complexity also increases the potential for transient storage (both surface and subsurface) and influences stream temperature. Impacts of beaver dams and beaver activity on stream responses are difficult to quantify because responses are dynamic and spatially variable. Few studies have focused on the reach scale temporal influences on stream responses and further research is needed particularly in quantifying the influence of beaver dams and their role in shaping the stream habitat. This study explores the changing hydrology and temperature regime of Curtis Creek, a mountainous stream located in Northern Utah, in a 560 m long reach where groundwater exchanges and temperature differences were observed over a three-year period. We have collected continuous stream discharge, stream temperature data and performed tracer experiments. During the first year, we were able to capture the pre-beaver activity. In the second year, we captured the impacts of some beaver activity with only a few dams built in the reach, while the third year included the effects of an entire active beaver colony. By the end of the study period, a single thread channel had been transformed into a channel with side channels and backwaters at multiple locations therefore increasing channel complexity. The cumulative influence of beaver dams on reach scale discharge resulted in a slightly losing reach that developed into a gaining reach. At the smaller sub-reach scale, both losing to gaining and gaining to losing transformations were observed. Temperature differences showed a warming effect of beaver dams at the reach scale. The reach stream temperature difference increased on average 0.3°C when comparing the first to the third year of our study period. This warming trend was more pronounced in summer stream temperatures where differences were about 0.7°C. During winter months cooling was observed and temperatures decreased about -0.2°C over the reach. Annual tracer studies also captured an 81min (238%) increase in residence times due primarily to the increased channel complexity and storage over the three-year period. Our study provides reach scale understanding regarding the temporal influence of beavers to not only change physical template of the channel, but also influence the hydrology and temperature regime of streams.

  15. Thermal energy storage with geothermal triplet for space heating and cooling

    NASA Astrophysics Data System (ADS)

    Bloemendal, Martin; Hartog, Niels

    2017-04-01

    Many governmental organizations and private companies have set high targets in avoiding CO2 emissions and reducing energy (Kamp, 2015; Ministry-of-Economic-affairs, 2016). ATES systems use groundwater wells to overcome the discrepancy in time between the availability of heat (during summer) and the demand for heat (during winter). Aquifer Thermal Energy Storage is an increasingly popular technique; currently over 2000 ATES systems are operational in the Netherlands (Graaf et al., 2016). High temperature ATES may help to improve performance of these conventional ATES systems. ATES systems use heat pumps to get the stored heat to the required temperature for heating of around 40-50°C and to produce the cold water for cooling in summer. These heat pumps need quite a lot of power to run; on average an ATES system produces 3-4 times less CO2 emission compared to conventional. Over 60% of those emission are accounted for by the heat pump (Dekker, 2016). This heat pump power consumption can be reduced by utilizing other sources of sustainable heat and cooling capacity for storage in the subsurface. At such operating temperatures the required storage temperatures do no longer match the return temperatures in the building systems. Therefore additional components and an additional well are required to increase the groundwater temperature in summer (e.g. solar collectors) and decrease it in winter (e.g. dry coolers). To prevent "pollution" of the warm and cold well return water from the building can be stored in a third well until weather conditions are suitable for producing the required storage temperature. Simulations and an economical evaluation show great potential for this type of aquifer thermal energy storage; economic performance is better than normal ATES while the emissions are reduce by a factor ten. At larger temperature differences, also the volume of groundwater required to pump around is much less, which causes an additional energy saving. Research now focusses on energy balance and energy loss in the subsurface, well design requirements, working/operational conditions of each well, as well as building system components like the influence of weather conditions on performance of system components. At EGU we like to present and discuss the results of this research. references • Dekker, L.d., 2016. Bepalende factoren voor goed functionerende WKO, kennisplatform bodemenergie. • Graaf, A.d., Heijer, R., Postma, S., 2016. Evaluatie Wijzigingsbesluit bodemenergiesystemen. Buro 38 in commision of ministry of Intrastructure and environment, Cothen. • Kamp, H., 2015. Warmtevisie, ministry of economic affairs, Den Haag. • Ministry-of-Economic-affairs, 2016. Energieagenda, Naar een CO₂-arme energievoorziening. Ministry of Economic affairs, Den Haag.

  16. Effects of storage time and temperature on lipid oxidation of egg powders enriched with natural antioxidants.

    PubMed

    Matumoto-Pintro, Paula Toshimi; Murakami, Alice Eiko; Vital, Ana Carolina Pelaes; Croge, Camila; da Silva, Denise Felix; Ospina-Roja, Ivan Camilo; Guerra, Ana Flávia Quiles Garcia

    2017-08-01

    The lipid fraction of egg powder may be affected by storage conditions due to the development of oxidative rancidity caused by polyunsaturated fatty acids. This study evaluated egg powders enriched with antioxidants [tocopherol, catechin, lycopene, and butylated hydroxyanisole (BHA)] for conjugated dienes (during a 90-day period) and for malonaldehydes (during a 210-day period) at 25±2 and 4±1°C. The presence of lycopene and BHA increases the total phenolic compounds in the enriched egg powders, and BHA exhibits the most antioxidant activity, as quantified by an ABTS assay. Egg powders enriched with antioxidants do not show any reduction in conjugate diene production compared to controls, and no effect of storage temperature is observed; however, in the production of malonaldehyde, greater stability is observed at 4°C, and catechin is more effective in reducing oxidation during storage. The results show that natural antioxidants can be used in egg powder instead of synthetic compounds to reduce malonaldehyde production during storage. Copyright © 2017 Elsevier Ltd. All rights reserved.

  17. Comparative assessment of software for non-targeted data analysis in the study of volatile fingerprint changes during storage of a strawberry beverage.

    PubMed

    Morales, M L; Callejón, R M; Ordóñez, J L; Troncoso, A M; García-Parrilla, M C

    2017-11-03

    Five free software packages were compared to assess their utility for the non-targeted study of changes in the volatile profile during the storage of a novel strawberry beverage. AMDIS coupled to Gavin software turned out to be easy to use, required the minimum handling for subsequent data treatment and its results were the most similar to those obtained by manual integration. However, AMDIS coupled to SpectConnect software provided more information for the study of volatile profile changes during the storage of strawberry beverage. During storage, volatile profile changed producing the differentiation among the strawberry beverage stored at different temperatures, and this difference increases as time passes; these results were also supported by PCA. As expected, it seems that cold temperature is the best way of preservation for this product during long time storage. Variable Importance in the Projection (VIP) and correlation scores pointed out four volatile compounds as potential markers for shelf-life of our strawberry beverage: 2-phenylethyl acetate, decanoic acid, γ-decalactone and furfural. Copyright © 2017 Elsevier B.V. All rights reserved.

  18. Effects of postmortem storage temperature on sea bass (Dicentrarchus labrax) muscle protein degradation: analysis by 2-D DIGE and MS.

    PubMed

    Terova, Genciana; Addis, Maria Filippa; Preziosa, Elena; Pisanu, Salvatore; Pagnozzi, Daniela; Biosa, Grazia; Gornati, Rosalba; Bernardini, Giovanni; Roggio, Tonina; Saroglia, Marco

    2011-07-01

    Storage conditions are known to be important for postmortem deterioration of fish muscle, and temperature is one of the factors with the strongest impact on this process. In order to shed light on the influence of temperature on the status of sea bass (Dicentrarchus labrax) muscle proteins during postmortem storage, a 2-D DIGE and mass spectrometry study was performed on fish kept at either 1 or 18°C for 5 days. As expected, the greatest alterations in sea bass filet protein composition were observed upon postmortem storage at 18°C, with distinct changes appearing in the 2-D protein profile after 5 days of storage at this temperature. In particular, degradation of the myofibrillar protein myosin heavy chain and of the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase, among the most abundant muscle proteins, could be clearly observed upon storage at higher temperatures. Although to a lesser extent, however, several proteins were observed to vary in abundance also upon storage for 5 days at 1°C. In particular, one of the most interesting observations was the rapid and significant decrease in the abundance of nucleoside diphosphate kinase B and phosphoglycerate mutase 2, which was observed also at low storage temperatures and appeared to be temperature-independent. The results of this study offer new knowledge on changes occurring in sea bass muscle proteins during postmortem storage at different temperatures and provide indications on protein degradation trends that might be useful for monitoring freshness of fish and quality of storage conditions. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  19. Effect of storage temperature on cultured epidermal cell sheets stored in xenobiotic-free medium.

    PubMed

    Jackson, Catherine; Aabel, Peder; Eidet, Jon R; Messelt, Edward B; Lyberg, Torstein; von Unge, Magnus; Utheim, Tor P

    2014-01-01

    Cultured epidermal cell sheets (CECS) are used in regenerative medicine in patients with burns, and have potential to treat limbal stem cell deficiency (LSCD), as demonstrated in animal models. Despite widespread use, short-term storage options for CECS are limited. Advantages of storage include: flexibility in scheduling surgery, reserve sheets for repeat operations, more opportunity for quality control, and improved transportation to allow wider distribution. Studies on storage of CECS have thus far focused on cryopreservation, whereas refrigeration is a convenient method commonly used for whole skin graft storage in burns clinics. It has been shown that preservation of viable cells using these methods is variable. This study evaluated the effect of different temperatures spanning 4°C to 37°C, on the cell viability, morphology, proliferation and metabolic status of CECS stored over a two week period in a xenobiotic-free system. Compared to non-stored control, best cell viability was obtained at 24°C (95.2±9.9%); reduced cell viability, at approximately 60%, was demonstrated at several of the temperatures (12°C, 28°C, 32°C and 37°C). Metabolic activity was significantly higher between 24°C and 37°C, where glucose, lactate, lactate/glucose ratios, and oxygen tension indicated increased activation of the glycolytic pathway under aerobic conditions. Preservation of morphology as shown by phase contrast and scanning electron micrographs was best at 12°C and 16°C. PCNA immunocytochemistry indicated that only 12°C and 20°C allowed maintenance of proliferative function at a similar level to non-stored control. In conclusion, results indicate that 12°C and 24°C merit further investigation as the prospective optimum temperature for short-term storage of cultured epidermal cell sheets.

  20. The Effect of Acclimation to Sublethal Temperature on Subsequent Susceptibility of Sitophilus zeamais Mostchulsky (Coleoptera: Curculionidae) to High Temperatures

    PubMed Central

    Lü, Jianhua; Zhang, Huina

    2016-01-01

    Heat treatment is a popular alternative to synthetic pesticides in disinfesting food-processing facilities and empty grain storages. Sitophilus zeamais Mostchulsky is one of the most cosmopolitan and destructive insects found in empty grain storage facilities and processing facilities. The effect of acclimation in S. zeamais adults to sublethal high temperature on their subsequent susceptibility to high temperatures was investigated. S. zeamais adults were acclimated to 36°C for 0 (as a control), 1, 3, and 5 h, and then were exposed at 43, 47, 51, and 55°C for different time intervals respectively. Acclimation to sublethal high temperature significantly reduced subsequent susceptibility of S. zeamais adults to lethal high temperatures of 43, 47, 51, and 55°C, although the mortality of S. zeamais adults significantly increased with increasing exposure time at lethal high temperatures. The mortality of S. zeamais adults with 1, 3, and 5 h of acclimation to 36°C was significantly lower than that of S. zeamais adults without acclimation when exposed to the same lethal high temperatures. The present results suggest that the whole facility should be heated to target lethal high temperature as soon as possible, avoiding decreasing the control effectiveness of heat treatment due to the acclimation in stored product insects to sublethal temperature. PMID:27462906

  1. Effect of high night temperature on storage lipids and transcriptome changes in developing seeds of oilseed rape.

    PubMed

    Zhou, Longhua; Yan, Tao; Chen, Xin; Li, Zhilan; Wu, Dezhi; Hua, Shuijin; Jiang, Lixi

    2018-03-24

    Global warming causes a faster increase of night temperature than of day temperature in tropical and subtropical zones. Little is known about the effect of high night temperature on storage lipids and transcriptome changes in oilseed rape. This study compared the total fatty acids and fatty acid compositions in seeds of two oilseed rape cultivars between high and low night temperatures. Their transcriptome profiles were also analyzed. High night temperature significantly affected the total fatty acids and fatty acid compositions in seeds of both low and high oil content cultivars, namely Jiuer-13 and Zheyou-50, thereby resulting in 18.9% and 13.7% total fatty acid reductions, respectively. In particular, high night temperature decreased the relative proportions of C18:0 and C18:1 but increased the proportions of C18:2 and C18:3 in both cultivars. In-depth analysis of transcriptome profiles revealed that high night temperature up-regulated gibberellin signaling during the night-time. This up-regulation was associated with the active expression of genes involved in fatty acid catabolism, such as those in β-oxidation and glyoxylate metabolism pathways. Although the effect of temperature on plant lipids has been previously examined, the present study is the first to focus on night temperature and its effect on the fatty acid composition in seeds.

  2. Relationship between environmental factors, dry matter loss and mycotoxin levels in stored wheat and maize infected with Fusarium species.

    PubMed

    Mylona, Kalliopi; Sulyok, Michael; Magan, Naresh

    2012-01-01

    This study examined the relationship between storage environmental factors (water activity (a(w)) (0.89-0.97) and temperature (15°C-30°C)), colonisation of wheat and maize by Fusarium graminearum and F. verticillioides respectively and the dry matter losses (DMLs) caused and quantified by contamination with deoxynivalenol (DON), zearalenone (ZEA) and fumonisins (FUMs) during storage. Fungal growth was assessed by the amount of CO(2) produced under different interacting conditions of a(w) and temperature. DMLs were quantified using the cumulative CO(2) data, and these were shown to increase as temperature and a(w) increased. The amount of DON, ZEA (wheat for human consumption) and FUMs (feed maize) produced was significantly affected by the storage conditions. The three toxins however showed different patterns of production. Optimum for DON was at the wettest conditions (0.97a(w)) and the highest temperature assessed (30°C), whereas for ZEA this shifted to 25°C. FUMs were produced in higher amounts in maize at 30°C and 0.97a(w); however, at intermediate a(w) levels (0.955a(w)), the highest production occurred at 25°C followed by 20°C. Polynomial models were developed for the effect of the storage factors on DMLs and toxin production. DMLs under different environmental conditions were significantly correlated with DON and FUMs. DON contamination was above the EU limits in at least 80% of the wheat samples with DMLs >1%, whereas at least 70% of the same samples contained ZEA above the respective EU legislative limits. Similarly, at least 75% of the maize samples with DMLs ≥ 0.9% exceeded the EU limits for the sum of FUMs in feed. These results show that it may be possible to use temporal CO(2) production during storage of grains as an indicator of the level of contamination of the grain with mycotoxins.

  3. Aquifer Thermal Energy Storage : A Well Doublet Experiment at Increased Temperatures

    NASA Astrophysics Data System (ADS)

    Molz, F. J.; Melville, J. G.; Parr, A. D.; King, D. A.; Hopf, M. T.

    1983-02-01

    The two main objectives of this communication are to present a study of potential advantages and disadvantages of the doublet supply-injection well configuration in an aquifer thermal energy storage (ATES) system and to report on aquifer storage problems with injection temperatures in the 80°C range. A 3-month injection-storage-recovery cycle followed by a 7.3-month cycle constituted the main experiment. The injection volumes were 25,402 m3 and 58,063 m3 at average temperatures of 58.5°C and 81°C respectively. Unlikely previous experiments at the Mobile site, no clogging of the injection well due to clay particle swelling, dispersion, and migration was observed. This is attributed to the fact that the supply water used for injection contained a cation concentration equal to or slightly greater than that in the native groundwater. For cycles I and II, the fraction of injected energy recovered in a volume of water equal to the injection volume was 0.56 and 0.45 respectively. Both groundwater temperature and tracer data support the conclusion that this relatively low recovery was due to the detrimental effects of free thermal convection, possibly augmented by longitudinal zones of high permeability. Construction of a partially penetrating recovery well improved recovery efficiency but is not thought to be an adequate solution to thermal stratification. A maximum increase of 1.24 cm in relative land surface elevation was recorded near the end of second cycle injection. The engineering implications of such an elevation change would have to be considered, especially if an ATES system were being designed in an urban environment. A third cycle was started at the Mobile site on April 7, 1982. This final experiment contains a partially penetrating, dual-recovery well system which is expected to maximize energy recovery from a thermally stratified storage aquifer.

  4. Recording and reading temperature tolerance in holographic data storage, in relation to the anisotropic thermal expansion of a photopolymer medium.

    PubMed

    Tanaka, Tomiji

    2009-08-03

    In holographic data storage, it is difficult to retrieve data if the temperature difference between recording and reading exceeds 2 K. To widen this tolerance, a compensation method--adjusting the wavelengths and incident directions of the recording and reading beams--has been proposed. In this paper, for the first time, a method for calculating the recording and reading temperature tolerance using this compensation is introduced. To widen the narrow tolerance, typically +/- 10 K, it is effective to increase the coefficient of thermal expansion (CTE) of the substrate or decrease the CTE of the photopolymer. Although reducing the Numerical aperture of the objective lens is also effective, it degrades the recording density.

  5. Biogas generation from in-storage psychrophilic anaerobic digestion.

    PubMed

    Giard, David; Choiniere, Denis; Cordeau, Sébastien; Barrington, Suzelle

    2013-01-01

    In-storage psychrophilic anaerobic digestion (ISPAD) is a technology allowing livestock producers to operate an anaerobic digester with minimum technological know-how and for the cost of a conventional storage cover. Nevertheless, the system is exposed to ambient temperatures and biogas production is expected to vary with climatic conditions. The objective of the project was therefore to measure ISPAD biogas production during the winter and fall seasons for a region east of Montreal, Canada. A calibrated biogas monitoring system was used to monitor biogas methane and carbon dioxide concentrations inside a two-year-old field installation with a 1000 m3 storage capacity. Despite a leaking pumping hatch, winter 2010 (January to March) methane concentrations varied directly with solar radiation and maximum exterior temperature, rather than with manure temperature at 2.4 and 1.2 m depths which remained relatively constant between 1 and 5 degrees C. During a six-month-period from November 2009 to April 2010, inclusively, the field ISPAD degraded 34% of the manure volatile solids corresponding to an average methane production of 40 m3/d. The ISPAD biogas production could be further increased by improving its air tightness and intrusion and by regularly pumping out the biogas.

  6. Enhanced Stability of Inactivated Influenza Vaccine Encapsulated in Dissolving Microneedle Patches

    PubMed Central

    Chu, Leonard Y.; Ye, Ling; Dong, Ke; Compans, Richard W.; Yang, Chinglai; Prausnitz, Mark R.

    2015-01-01

    Purpose This study tested the hypothesis that encapsulation of influenza vaccine in microneedle patches increases vaccine stability during storage at elevated temperature. Methods Whole inactivated influenza virus vaccine (A/Puerto Rico/8/34) was formulated into dissolving microneedle patches and vaccine stability was evaluated by in vitro and in vivo assays of antigenicity and immunogenicity after storage for up to 3 months at 4, 25, 37 and 45°C. Results While liquid vaccine completely lost potency as determined by hemagglutination (HA) activity within 1–2 weeks outside of refrigeration, vaccine in microneedle patches lost 40–50% HA activity during or shortly after fabrication, but then had no significant additional loss of activity over 3 months of storage, independent of temperature. This level of stability required reduced humidity by packaging with desiccant, but was not affected by presence of oxygen. This finding was consistent with additional stability assays, including antigenicity of the vaccine measured by ELISA, virus particle morphological structure captured by transmission electron microscopy and protective immune responses by immunization of mice in vivo. Conclusions These data show that inactivated influenza vaccine encapsulated in dissolving microneedle patches has enhanced stability during extended storage at elevated temperatures. PMID:26620313

  7. Effects of hexanal, trans-2-hexenal, and storage temperature on shelf life of fresh sliced apples.

    PubMed

    Corbo, M R; Lanciotti, R; Gardini, F; Sinigaglia, M; Guerzoni, M E

    2000-06-01

    In this paper, the effects of hexanal and trans-2-hexenal, which are both natural molecules characterizing apple aroma, on the microbial population and on color retention of fresh sliced apples were evaluated. In particular, a central composite design (CCD) was developed to assess the individual and interactive effects of the chosen volatile molecules and storage temperatures on (i) the growth of the naturally occurring microflora, (ii) the evolution over time of an inoculated spoilage yeast (Pichia subpelliculosa), and (iii) the enzymatic browning reaction in minimally processed apples. The inclusion of hexanal and trans-2-hexenal in the storage atmosphere of apple slices determined a significant extension of shelf life also when P. subpelliculosa was inoculated at levels of 10(3) colony-forming units/g and abusive storage temperatures were used. In fact, the presence of these molecules in the packaging atmospheres considerably prolonged the lag phases of the inoculated yeast and reduced the growth potential of naturally occurring bacteria. Moreover, the addition to the modified atmosphere of low levels of the hexanal increased the color stability of the products up to 16 days.

  8. Energy-storage properties and electrocaloric effect of Pb(1-3x/2)LaxZr0.85Ti0.15O3 antiferroelectric thick films.

    PubMed

    Zhao, Ye; Hao, Xihong; Zhang, Qi

    2014-07-23

    Antiferroelectric (AFE) thick (1 μm) films of Pb(1-3x/2)LaxZr0.85Ti0.15O3 (PLZT) with x = 0.08, 0.10, 0.12, and 0.14 were deposited on LaNiO3/Si (100) substrates by a sol-gel method. The dielectric properties, energy-storage performance, electrocaloric effect, and leakage current behavior were investigated in detail. With increasing La content, dielectric constant and saturated polarizations of the thick films were gradually decreased. A maximum recoverable energy-storage density of 38 J/cm(3) and efficiency of 71% were achieved in the thick films with x = 0.12 at room temperature. A large reversible adiabatic temperature change of ΔT = 25.0 °C was presented in the thick films with x = 0.08 at 127 °C at 990 kV/cm. Moreover, all the samples had a lower leakage current density below 10(-6) A/cm(2) at room temperature. These results indicated that the PLZT AFE thick films could be a potential candidate for applications in high energy-storage density capacitors and cooling devices.

  9. Variability and Change in Seasonal Water Storage in the Major Arctic Draining Eurasian River Systems

    NASA Astrophysics Data System (ADS)

    Serreze, M. C.; Barrett, A. P.

    2015-12-01

    Variability and change in seasonal water storage in the major Arctic-draining watersheds of Eurasia (Ob. Yenisei and Lena) are assessed in several ways using a combination of storage estimates from the NASA GRACE satellite system, gauged runoff and output from the NASA MERRA atmospheric reanalysis. The study is motivated by the pronounced environmental changes observed in the northern high latitudes and recognition of the climatic importance of changes in hydrology both within and beyond the region. Monthly storage changes based on GRACE gravimetric measurements (2002-2015) and from a water balance approach for the same period calculating storage changes as a residual using gauged runoff along with aerologically-determined net precipitation (atmospheric vapor flux convergence minus the time change in atmospheric precipitable water) from MERRA are generally in good agreement. Agreement is also good for calculations in which aerologically-determined net precipitation is replaced with the MERRA forecasts of precipitation and evapotranspiration. On average, the storage in each of the three watersheds examined (the Ob, Yenisei and Lena) peaks in March and is at a minimum in September. However, this seasonal cycle, primarily driven by snowpack storage through autumn and winter, and snowmelt through spring and summer, varies considerably from year to year in amplitude, phase and between the three watersheds in response to variability in precipitation, evapotranspiration, and near surface air temperature. As assessed over the longer period 1979-2015 covered by MERRA, there is evidence that in response to rising air temperatures influencing precipitation phase and snow storage, peak storage has shifted to earlier in the winter. While recent work provides evidence for a link between increased autumn snowfall over Eurasia and reduced autumn sea ice extent that provides for a moisture source, the effect of increased snowfall is not clearly apparent in water storage.

  10. High temperature superconducting magnetic energy storage for future NASA missions

    NASA Technical Reports Server (NTRS)

    Faymon, Karl A.; Rudnick, Stanley J.

    1988-01-01

    Several NASA sponsored studies based on 'conventional' liquid helium temperature level superconductivity technology have concluded that superconducting magnetic energy storage has considerable potential for space applications. The advent of high temperature superconductivity (HTSC) may provide additional benefits over conventional superconductivity technology, making magnetic energy storage even more attractive. The proposed NASA space station is a possible candidate for the application of HTSC energy storage. Alternative energy storage technologies for this and other low Earth orbit missions are compared.

  11. Energy Storage of Polyarylene Ether Nitriles at High Temperature

    NASA Astrophysics Data System (ADS)

    Tang, Xiaohe; You, Yong; Mao, Hua; Li, Kui; Wei, Renbo; Liu, Xiaobo

    2018-03-01

    Polyarylene ether nitrile (PEN) was synthesized and used as film capacitors for energy storage at high temperature. Scanning electron microscopy observation indicated that the films of PEN have pinholes at nanoscales which restricted the energy storage properties of the material. The pinhole shadowing effect through which the energy storage properties of PEN were effectively improved to be 2.3 J/cm3 was observed by using the overlapped film of PEN. The high glass transition temperature (T g) of PEN was as high as 216 °C and PEN film showed stable dielectric constant, breakdown strength and energy storage density before the T g. The PEN films will be a potential candidate as high performance electronic storage materials used at high temperature.

  12. Natural Carbonation of Peridotite and Applications for Carbon Storage

    NASA Astrophysics Data System (ADS)

    Streit, E.; Kelemen, P.; Matter, J.

    2009-05-01

    Natural carbonation of peridotite in the Samail Ophiolite of Oman is surprisingly rapid and could be further enhanced to provide a safe, permanent method of CO2 storage through in situ formation of carbonate minerals. Carbonate veins form by low-temperature reaction between peridotite and groundwater in a shallow weathering horizon. Reaction with peridotite drives up the pH of the water, and extensive travertine terraces form where this groundwater emerges at the surface in alkaline springs. The potential sink for CO2 in peridotite is enormous: adding 1wt% CO2 to the peridotite in Oman could consume 1/4 of all atmospheric carbon, and several peridotite bodies of comparable size exist throughout the world. Thus carbonation rate and cost, not reservoir size, are the limiting factors on the usefulness of in situ mineral carbonation of peridotite for carbon storage. The carbonate veins in Oman are much younger than previously believed, yielding average 14C ages of 28,000 years. Age data plus estimated volumes of carbonate veins and terraces suggest 10,000 to 100,000 tons per year of CO2 are consumed by these peridotite weathering reactions in Oman. This rate can be enhanced by drilling, hydraulic fracture, injecting CO2-rich fluid, and increasing reaction temperature. Drilling and hydraulic fracture can increase volume of peridotite available for reaction. Additional fracture may occur due to the solid volume increase of the carbonation reaction, and field observations suggest that such reaction-assisted fracture may be responsible for hierarchical carbonate vein networks in peridotite. Natural carbonation of peridotite in Oman occurs at low pCO2, resulting in partial carbonation of peridotite, forming magnesite and serpentine. Raising pCO2 increases carbonation efficiency, forming of magnesite + talc, or at complete carbonation, magnesite + quartz, allowing ˜30wt% CO2 to be added to the peridotite. Increasing the temperature to 185°C can improve the reaction rate by a factor of more than 100,000. Thermal modeling suggests that after an initial heating stage, CO2-rich fluids injected at relatively low temperature can be heated by exothermic carbonation reactions, offsetting diffusive heat loss to maintain optimal temperatures for rapid carbonation without additional energy input. With these enhancements, in situ carbonation could consume more than 1 billion tons of CO2 per cubic kilometer of peridotite per year. Costs associated with this method include drilling, hydraulic fracture, initial heating, CO2 capture and transport, fluid injection and monitoring. The techniques for drilling, fracture and injection are routinely used by oil companies. Compared with other carbon storage methods, in situ mineral carbonation has several advantages. It offers permanent storage that is safer and easier to monitor than storage of CO2-rich fluids in porous underground reservoirs or in the ocean. It may also be less costly than ex situ mineral carbonation, which requires quarrying and transportation of peridotite, grinding and heat treatment, reactions in pressure vessels at elevated temperature, production of catalysts, and disposal of carbonated material. An alternative method, carbonation by reaction of offshore peridotite with shallow seawater rather than CO2-rich fluids, would consume less CO2, but would avoid the costs of CO2 capture and transport inherent in other CCS methods. Drilling to depths where rocks are already close to the optimal carbonation temperature would avoid pre-heating costs and circulate water by thermal convection rather than pumping fluids.

  13. Production system and storage temperature influence grapefruit vitamin C, limonoids, and carotenoids.

    PubMed

    Chebrolu, Kranthi K; Jayaprakasha, G K; Jifon, J; Patil, Bhimanagouda S

    2012-07-25

    Concentrations of grapefruit (cv. 'Rio Red'; Citrus paradisi Macf.) bioactives grown under organic and conventional production systems were evaluated after storage at various temperatures. The first experiment was conducted in November 2008 and the second experiment was conducted in February 2011 using commercial production, processing, and packing procedures. The harvested grapefruits were stored at 23 °C (room temperature) or 9 °C for 4 weeks and analyzed for vitamin C, limonoids, and carotenoids at the end of each week using HPLC. Vitamin C levels were higher in organically grown grapefruits (41.8 mg/100 g) compared to conventionally grown grapefruits (39.2 mg/100 g) at 0 days after harvest in the first experiment. However, production system did not significantly affect vitamin C levels in the second experiment. During storage at room temperature, vitamin C degradation losses ranged from 0.5 to 7% for organically produced grapefruits and from 3 to 18% for conventional grapefruits in both experiments. In the first experiment at harvest, organically produced grapefruits had 77% higher (p ≤ 0.05) nomilin than conventionally produced grapefruits, whereas grapefruits grown under the conventional production system had 2-fold higher lycopene levels compared to organic grapefruits. In the second experiment, both β-carotene and lycopene levels were significantly (p ≤ 0.05) higher in conventionally produced grapefruits than in organic grapefruits. Overall, conventional production significantly increased grapefruit carotenoid levels in both experiments. In general, storage temperature (room temperature and 9 °C) had minimal effects on vitamin C degradation but significant effects on the degradation of carotenoids in the first experiment.

  14. Cultured Human Retinal Pigment Epithelial (hRPE) Sheets: A Search for Suitable Storage Conditions.

    PubMed

    Khan, Ayyad Z; Utheim, Tor P; Reppe, Sjur; Sandvik, Leiv; Lyberg, Torstein; Roald, Borghild B-H; Ibrahim, Ibrahim B; Eidet, Jon R

    2018-04-01

    The advancement of human retinal pigment epithelial cell (hRPE) replacement therapy is partly dependent on optimization of cell culture, cell preservation, and storage medium. This study was undertaken to search for a suitable storage temperature and storage medium for hRPE. hRPE monolayer sheets were cultured under standard conditions at 37°C and then randomized for storage at six temperatures (4, 16, 20, 24, 28, and 37°C) for 7 days. After revealing a suitable storage temperature, hRPE sheets were subsequently stored with and without the silk protein sericin added to the storage medium. Live/dead assay, light microscopy, pH, and phenotypic expression of various proteins were used to assess cell cultures stored at different temperatures. After 7 days of storage, hRPE morphology was best preserved at 4°C. Addition of sericin to the storage medium maintained the characteristic morphology of the preserved cells, and improved pigmentation and levels of pigmentation-related proteins in the cultured hRPE sheets following a 7-day storage period at 4°C.

  15. A Preliminary Study on Rock Bed Heat Storage from Biomass Combustion for Rice Drying

    NASA Astrophysics Data System (ADS)

    Nelwan, L. O.; Wulandani, D.; Subrata, I. D. M.

    2018-05-01

    One of the main constraints of biomass fuel utilization in a small scale rice drying system is the operating difficulties related to the adjustment of combustion/feeding rate. Use of thermal storage may reduce the problem since combustion operation can be accomplished in a much shorter time and then the use of heat can be regulated by simply adjusting the air flow. An integrated biomass furnace-rock bed thermal storage with a storage volume of 540 L was designed and tested. There were four experiments conducted in this study. Charging was performed within 1-2 hours with a combustion rate of 11.5-15.5 kg/h. In discharging process, the mixing of air passing through the rock bed and ambient air were regulated by valves. Without adjusting the valve during the discharging process, air temperature increased up to 80°C, which is not suitable for rice batch drying process. Charging with sufficiently high combustion rate (14 kg/h) within 1 hour continued by adjusting the valve during discharging process below 60°C increased the discharge-charge time ratio (DCTR) up to 5.33 at average air temperature of 49°C and ambient temperature of 33°C.The efficiency of heat discharging was ranged from 34.5 to 45.8%. From the simulation, as much as 156.8-268.8 kg of rice was able to be dried by the discharging conditions.

  16. Effect of storage time on gene expression data acquired from unfrozen archived newborn blood spots.

    PubMed

    Ho, Nhan T; Busik, Julia V; Resau, James H; Paneth, Nigel; Khoo, Sok Kean

    2016-11-01

    Unfrozen archived newborn blood spots (NBS) have been shown to retain sufficient messenger RNA (mRNA) for gene expression profiling. However, the effect of storage time at ambient temperature for NBS samples in relation to the quality of gene expression data is relatively unknown. Here, we evaluated mRNA expression from quantitative real-time PCR (qRT-PCR) and microarray data obtained from NBS samples stored at ambient temperature to determine the effect of storage time on the quality of gene expression. These data were generated in a previous case-control study examining NBS in 53 children with cerebral palsy (CP) and 53 matched controls. NBS sample storage period ranged from 3 to 16years at ambient temperature. We found persistently low RNA integrity numbers (RIN=2.3±0.71) and 28S/18S rRNA ratios (~0) across NBS samples for all storage periods. In both qRT-PCR and microarray data, the expression of three common housekeeping genes-beta cytoskeletal actin (ACTB), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), and peptidylprolyl isomerase A (PPIA)-decreased with increased storage time. Median values of each microarray probe intensity at log 2 scale also decreased over time. After eight years of storage, probe intensity values were largely reduced to background intensity levels. Of 21,500 genes tested, 89% significantly decreased in signal intensity, with 13,551, 10,730, and 9925 genes detected within 5years, > 5 to <10years, and >10years of storage, respectively. We also examined the expression of two gender-specific genes (X inactivation-specific transcript, XIST and lysine-specific demethylase 5D, KDM5D) and seven gene sets representing the inflammatory, hypoxic, coagulative, and thyroidal pathways hypothesized to be related to CP risk to determine the effect of storage time on the detection of these biologically relevant genes. We found the gender-specific genes and CP-related gene sets detectable in all storage periods, but exhibited differential expression (between male vs. female or CP vs. control) only within the first six years of storage. We concluded that gene expression data quality deteriorates in unfrozen archived NBS over time and that differential gene expression profiling and analysis is recommended for those NBS samples collected and stored within six years at ambient temperature. Copyright © 2016 Elsevier Inc. All rights reserved.

  17. Antimicrobial activity of allyl isothiocyanate used to coat biodegradable composite films as affected by storage and handling conditions.

    PubMed

    Li, Weili; Liu, Linshu; Jin, Tony Z

    2012-12-01

    We evaluated the effects of storage and handling conditions on the antimicrobial activity of biodegradable composite films (polylactic acid and sugar beet pulp) coated with allyl isothiocyanate (AIT). Polylactic acid and chitosan were incorporated with AIT and used to coat one side of the film. The films were subjected to different storage conditions (storage time, storage temperature, and packed or unpacked) and handling conditions (washing, abrasion, and air blowing), and the antimicrobial activity of the films against Salmonella Stanley in tryptic soy broth was determined. The films (8.16 μl of AIT per cm(2) of surface area) significantly (P < 0.05) inhibited the growth of Salmonella during 24 h of incubation at 22°C, while the populations of Salmonella in controls increased from ca. 4 to over 8 log CFU/ml, indicating a minimum inactivation of 4 log CFU/ml on films in comparison to the growth on controls. Statistical analyses indicated that storage time, storage temperature, and surface abrasion affected the antimicrobial activity of the films significantly (P < 0.05). However, the differences in microbial reduction between those conditions were less than 0.5 log cycle. The results suggest that the films' antimicrobial properties are stable under practical storage and handling conditions and that these antimicrobial films have potential applications in food packaging.

  18. Analysis of older adults' domestic kitchen storage practices in the United Kingdom: identification of risk factors associated with listeriosis.

    PubMed

    Evans, Ellen W; Redmond, Elizabeth C

    2015-04-01

    Increased listeriosis incidence among older adults (≥ 60 years) has been reported internationally, with many cases reported to be sporadic and associated with ready-to-eat (RTE) food products with extended refrigerated shelf life. Given that the home kitchen is recognized as a significant location where foodborne illnesses are acquired, it is important that consumers implement safe food practices to minimize risks. This is crucial for vulnerable consumers, such as older adults. Consumer food safety recommendations in the United Kingdom to reduce the risk of listeriosis at home include (i) following "use-by" dates on unopened prepacked RTE food products, (ii) consuming RTE food products within 2 days of opening, and (iii) ensuring the safe operating temperatures of domestic refrigerators (≤ 5 °C). This study utilized observation, self-reporting, and microbiological analysis to determine actual food storage practices to identify behavioral risk factors. A domestic kitchen survey was conducted in older adult (≥ 60 years) consumers' domestic kitchens (n = 100) in South Wales, United Kingdom. Forty-one percent of foods in home refrigerators were beyond the use-by date, of which 11% were unopened RTE food products commonly associated with listeriosis. Sixty-six percent of opened RTE foods had been or were intended to be stored beyond the recommended 2 days after opening. Older adults failed to ensure safe refrigeration temperatures, with 50% of central storage and 85% of door storage areas operating at temperatures >5 °C. Older refrigerators operated at significantly (P < 0.05) higher temperatures. Given that Listeria monocytogenes was isolated in 2% of kitchens, these findings suggest that storage malpractices may have a greater effect on the potential risk of listeriosis than its presence alone. The study has determined that many older adults fail to adhere to recommendations and subject RTE foods associated with L. monocytogenes to prolonged storage at unsafe temperatures which may render food unsafe for consumption.

  19. Effect of starter culture, spice mix and storage time and temperature on biogenic amine content of dry fermented sausages.

    PubMed

    Komprda, T; Smělá, D; Pechová, P; Kalhotka, L; Stencl, J; Klejdus, B

    2004-08-01

    Two types of dry fermented sausage differing in spicing mixture and the diameter (low content of red pepper+diameter 80 mm, H-sausage; high content of red pepper+diameter 55 mm, P-sausage, respectively) were produced in parallel with two different starter cultures (Pediococcus pentosaceus+Staphylococcus carnosus, B-samples and S. carnosus+Staphylococcus xylosus+Lactobacillus farciminis, F-samples, respectively). The sausages were ripened 21 days and subsequently stored 91 days at the room temperature. Concentration of both most abundant amines, putrescine and tyramine (y; mg/kg DM) increased significantly (P<0.01) in HB-sausage during ripening (x; days): y=2.5+18.13x-0.3144x(2) (R(2)=0.99) and y=0.7+8.17x-0.1130x(2) (R(2)=0.99), and also during storage: y=127.3+5.123x (R(2)=0.79) and y=26.0+3.211x (R(2)=0.74), respectively. At the end of ripening, putrescine (247 mg/kg DM) and tyramine (123 mg/kg DM) content in the HB-sausage was higher (P<0.05) than in the PB-sausage (12 and 9 mg/kg DM, respectively), concentration of either of these amines was negligible (1 mg/kg DM) in either type of F-inoculated sausage. Both starter culture and sausage type influenced significantly (P<0.001) both putrescine and tyramine content in the sausage; starter accounted for 57% and 55% of total variability in putrescine and tyramine content, respectively. Due to the significant (P<0.05) increase of total aerobic counts in the HB-sausage between the end of ripening and the 7th day of storage, followed by the significant (P<0.01) increase of the sum of total biogenic amines between the end of ripening (425 mg/kg DM) and the end of storage (1029 mg/kg DM), the storage of the dry fermented sausages at the room temperature should not be recommended.

  20. Influence of storage temperature and time on the physicochemical and bioactive properties of roselle-fruit juice blends in plastic bottle

    PubMed Central

    Mgaya-Kilima, Beatrice; Remberg, Siv Fagertun; Chove, Bernard Elias; Wicklund, Trude

    2014-01-01

    Roselle-fruit juice blends were made from roselle extract and mango, papaya, and guava juices at the ratio of 80:20, 60:40, 40:60, and 20:80, % roselle: fruit juice, respectively. The blends were pasteurized at 82.5°C for 20 min and stored in 100 mL plastic bottles at 28 and 4°C for 6 months. The effects of storage time and temperature on physicochemical and bioactive properties were evaluated. Total soluble solids, pH, and reducing sugars increased significantly (P < 0.05) in some blends while titratable acidity decrease with increasing storage time. Vitamin C, total monomeric anthocyanins (TMA), total phenols (TPC), and antioxidant activity (ferric reducing ability of plasma, FRAP) in all roselle-fruit blends (40% roselle) decreased significantly (P < 0.05) at 28 and 4°C as storage progressed. Vitamin C in all roselle-fruit blends (40% roselle) decreased from 58–55% to 43–42% when stored at 28 and 4°C, respectively. TMA losses were 86–65% at 28°C and 75–53% at 4°C while TPC losses were 66–58% at 28°C and 51–22% at 4°C. Loss of antioxidant capacity (FRAP) was 18–46% at 28°C and 17–35% at 4°C. A principal component analysis (PCA) differentiated roselle-juice fruit blends into two clusters with two principle components PC1 and PC2, which explained 97 and 3% (blends stored at ambient temperature) and 96 and 4% (blends stored at refrigerated temperature) of the variation, respectively. PC1 differentiated roselle-guava juice blends which were characterized by vitamin C, TPC, FRAP, and pH, while PC2 from another cluster of roselle-mango and roselle-papaya juice blends and was characterized by TSS, RS, and color parameters (L* a* b*). However, TMA was the main variable with the highest effect on all roselle-fruit juice blends regardless of the storage time and temperature. PMID:24804077

  1. Responses of two nonlinear microbial models to warming and increased carbon input

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Wang, Y. P.; Jiang, J.; Chen-Charpentier, Benito

    A number of nonlinear microbial models of soil carbon decomposition have been developed. Some of them have been applied globally but have yet to be shown to realistically represent soil carbon dynamics in the field. A thorough analysis of their key differences is needed to inform future model developments. In this paper, we compare two nonlinear microbial models of soil carbon decomposition: one based on reverse Michaelis–Menten kinetics (model A) and the other on regular Michaelis–Menten kinetics (model B). Using analytic approximations and numerical solutions, we find that the oscillatory responses of carbon pools to a small perturbation in theirmore » initial pool sizes dampen faster in model A than in model B. Soil warming always decreases carbon storage in model A, but in model B it predominantly decreases carbon storage in cool regions and increases carbon storage in warm regions. For both models, the CO 2 efflux from soil carbon decomposition reaches a maximum value some time after increased carbon input (as in priming experiments). This maximum CO 2 efflux (F max) decreases with an increase in soil temperature in both models. However, the sensitivity of F max to the increased amount of carbon input increases with soil temperature in model A but decreases monotonically with an increase in soil temperature in model B. These differences in the responses to soil warming and carbon input between the two nonlinear models can be used to discern which model is more realistic when compared to results from field or laboratory experiments. Lastly, these insights will contribute to an improved understanding of the significance of soil microbial processes in soil carbon responses to future climate change.« less

  2. Responses of two nonlinear microbial models to warming and increased carbon input

    DOE PAGES

    Wang, Y. P.; Jiang, J.; Chen-Charpentier, Benito; ...

    2016-02-18

    A number of nonlinear microbial models of soil carbon decomposition have been developed. Some of them have been applied globally but have yet to be shown to realistically represent soil carbon dynamics in the field. A thorough analysis of their key differences is needed to inform future model developments. In this paper, we compare two nonlinear microbial models of soil carbon decomposition: one based on reverse Michaelis–Menten kinetics (model A) and the other on regular Michaelis–Menten kinetics (model B). Using analytic approximations and numerical solutions, we find that the oscillatory responses of carbon pools to a small perturbation in theirmore » initial pool sizes dampen faster in model A than in model B. Soil warming always decreases carbon storage in model A, but in model B it predominantly decreases carbon storage in cool regions and increases carbon storage in warm regions. For both models, the CO 2 efflux from soil carbon decomposition reaches a maximum value some time after increased carbon input (as in priming experiments). This maximum CO 2 efflux (F max) decreases with an increase in soil temperature in both models. However, the sensitivity of F max to the increased amount of carbon input increases with soil temperature in model A but decreases monotonically with an increase in soil temperature in model B. These differences in the responses to soil warming and carbon input between the two nonlinear models can be used to discern which model is more realistic when compared to results from field or laboratory experiments. Lastly, these insights will contribute to an improved understanding of the significance of soil microbial processes in soil carbon responses to future climate change.« less

  3. Rising Mean Annual Temperature Increases Carbon Flux and Alters Partitioning, but Does Not Change Ecosystem Carbon Storage in Hawaiian Tropical Montane Wet Forest

    NASA Astrophysics Data System (ADS)

    Litton, C. M.; Giardina, C. P.; Selmants, P.

    2014-12-01

    Terrestrial ecosystem carbon (C) storage exceeds that in the atmosphere by a factor of four, and represents a dynamic balance among C input, allocation, and loss. This balance is likely being altered by climate change, but the response of terrestrial C cycling to warming remains poorly quantified, particularly in tropical forests which play a disproportionately large role in the global C cycle. Over the past five years, we have quantified above- and belowground C pools and fluxes in nine permanent plots spanning a 5.2°C mean annual temperature (MAT) gradient (13-18.2°C) in Hawaiian tropical montane wet forest. This elevation gradient is unique in that substrate type and age, soil type, soil water balance, canopy vegetation, and disturbance history are constant, allowing us to isolate the impact of long-term, whole ecosystem warming on C input, allocation, loss and storage. Across the gradient, soil respiration, litterfall, litter decomposition, total belowground C flux, aboveground net primary productivity, and estimates of gross primary production (GPP) all increase linearly and positively with MAT. Carbon partitioning is dynamic, shifting from below- to aboveground with warming, likely in response to a warming-induced increase in the cycling and availability of soil nutrients. In contrast to observed patterns in C flux, live biomass C, soil C, and total ecosystem C pools remained remarkably constant with MAT. There was also no difference in soil bacterial taxon richness, phylogenetic diversity, or community composition with MAT. Taken together these results indicate that in tropical montane wet forests, increased temperatures in the absence of water limitation or disturbance will accelerate C cycling, will not alter ecosystem C storage, and will shift the products of photosynthesis from below- to aboveground. These results agree with an increasing number of studies, and collectively provide a unique insight into anticipated warming-induced changes in tropical forest C cycling.

  4. Effects of sodium caseinate concentration and storage conditions on the oxidative stability of oil-in-water emulsions.

    PubMed

    O' Dwyer, Sandra P; O' Beirne, David; Eidhin, Deirdre Ní; O' Kennedy, Brendan T

    2013-06-01

    The oxidative stability of various oils (sunflower, camelina and fish) and 20% oil-in-water (O/W) emulsions, were examined. The mean particle size decreased from 1179 to 325 nm as sodium caseinate (emulsifier) concentration was increased from 0.25% to 3% in O/W emulsions (P<0.05). Increasing the microfluidisation pressure from 21 to 138 MPa, resulted in a particle size decrease from 289 to 194 nm (P<0.05). Emulsified oils had lower detectable lipid hydroperoxide and p-Anisidine values than their corresponding bulk oils (P<0.05). The lipid hydroperoxide and p-Anisidine values of emulsions generally decreased as sodium caseinate concentration increased, and similarly decreased as microfluidisation pressure increased (P<0.05). Increasing storage temperature of the emulsions from 5 to 60°C, resulted in lower detectable lipid oxidation products during storage (P<0.05). Copyright © 2012 Elsevier Ltd. All rights reserved.

  5. Effect of storage temperature on physico-chemical and sensory attributes of purple passion fruit (Passiflora edulis Sims).

    PubMed

    Kishore, Kundan; Pathak, K A; Shukla, Rohit; Bharali, Rinku

    2011-08-01

    Physico-chemical and sensory quality of juice from purple passion fruit under different storage temperature and time were assessed. The maximum loss in fruit weight was recorded under room temperature (25 ± 1°C) followed by at 11 ± 1°C. There was an increase in juice percentage up to 9 and 13 days under room temperature and storage at 11 ± 1°C respectively. The optimum flavour in juice was up to 5 days at 25 ± 1°C and up to 21 days at 8 ± 1°C. A significant reduction in sourness was recorded on 5th day under all treatments and the scores for sourness became almost constant after 17 days. The maximum increase in the mean scores of sweetness on 5th day was observed at 25 ± 1°C followed by at 11 ± 1°C. The optimum level of juice sweetness was maintained up to 21 days at 8 ± 1°C. Total soluble solids content increased in initial stage followed by reduction. There was a reduction in the titrable acidity up to 21 days at 8 ± 1°C. A decreasing trend in the reducing and non-reducing sugar of passion fruit was observed under all the treatments. Fruits stored at 25 ± 1°C, developed off-flavour in juice after 5 days, while storage at 8 ± 1°C produced no off-flavor even up to 21 days. Fruits can be stored for 5 days only at 25 ± 1°C as the overall sensory quality of juice reduced significantly afterwards, while juice maintained the optimum overall quality up to 21 days at 8 ± 1°C.

  6. Improved thermal storage material for portable life support systems

    NASA Technical Reports Server (NTRS)

    Kellner, J. D.

    1975-01-01

    The availability of thermal storage materials that have heat absorption capabilities substantially greater than water-ice in the same temperature range would permit significant improvements in performance of projected portable thermal storage cooling systems. A method for providing increased heat absorption by the combined use of the heat of solution of certain salts and the heat of fusion of water-ice was investigated. This work has indicated that a 30 percent solution of potassium bifluoride (KHF2) in water can absorb approximately 52 percent more heat than an equal weight of water-ice, and approximately 79 percent more heat than an equal volume of water-ice. The thermal storage material can be regenerated easily by freezing, however, a lower temperature must be used, 261 K as compared to 273 K for water-ice. This work was conducted by the United Aircraft Research Laboratories as part of a program at Hamilton Standard Division of United Aircraft Corporation under contract to NASA Ames Research Center.

  7. Characterization of four Paenibacillus species isolated from pasteurized, chilled ready-to-eat meals.

    PubMed

    Helmond, Mariette; Nierop Groot, Masja N; van Bokhorst-van de Veen, Hermien

    2017-07-03

    Food spoilage is often caused by microorganisms. The predominant spoilage microorganisms of pasteurized, chilled ready-to-eat (RTE) mixed rice-vegetable meals stored at 7°C were isolated and determined as Paenibacillus species. These sporeforming psychrotrophic bacteria are well adapted to grow in the starch-rich environment of pasteurized and chilled meals. Growth of the Paenibacillus isolates appeared to be delayed by decreased (<7°C) temperature or chilled temperature (7°C) combined with decreased pH (<5), increased sodium chloride (>5.5%, corresponding with an a w <0.934), or decreased a w (<0.931; using sucrose). To gain insight in the effect of the pasteurization processing of the meal on spore inactivation, heat-inactivation kinetics were determined and D-values were calculated. According to these kinetics, pasteurization up to 90°C, necessary for inactivation of vegetative spoilage microorganisms and pathogens, does not significantly contribute to the inactivation of Paenibacillus spores in the meals. Furthermore, outgrowth of pasteurized spores was determined in the mixed rice-vegetable meal at several temperatures; P. terrae FBR-61 and P. pabuli FBR-75 isolates did not substantially increase in numbers during storage at 2°C, but had a significant increase within a month of storage at 4°C or within several days at 22°C. Overall, this work shows the importance of Paenibacillus species as spoilage microorganisms of pasteurized, chilled RTE meals and that the meals' matrix, processing conditions, and storage temperature are important hurdles to control microbial meal spoilage. Copyright © 2017 Elsevier B.V. All rights reserved.

  8. Thermal energy storage devices, systems, and thermal energy storage device monitoring methods

    DOEpatents

    Tugurlan, Maria; Tuffner, Francis K; Chassin, David P.

    2016-09-13

    Thermal energy storage devices, systems, and thermal energy storage device monitoring methods are described. According to one aspect, a thermal energy storage device includes a reservoir configured to hold a thermal energy storage medium, a temperature control system configured to adjust a temperature of the thermal energy storage medium, and a state observation system configured to provide information regarding an energy state of the thermal energy storage device at a plurality of different moments in time.

  9. Rheology of polyaniline-dinonylnaphthalene disulfonic acid (DNNDSA) montmorillonite clay nanocomposites in the sol state: shear thinning versus pseudo-solid behavior.

    PubMed

    Garai, Ashesh; Nandi, Arun K

    2008-04-01

    The melt rheology of polyaniline (PANI)-dinonylnaphthalenedisulfonic acid (DNNDSA) gel nanocomposites (GNCs) with organically modified (modified with cetyl trimethylammonium bromide)-montmorillonite (om-MMT) clay has been studied for three different clay concentrations at the temperature range 120-160 degrees C. Field emission scanning electron microscopy (FE-SEM), wide angle X-ray scattering (WAXS), differential scanning calorimetry (DSC) and dc-conductivity data (approximately 10(-3) S/cm) indicate that the PANI-DNNDSA melt is in sol state and it is not de-doped at that condition. The WAXS data indicate that in GNC-1 sol clay tactoids are in exfoliated state but in the other sols they are in intercalated state. The zero shear viscosity (eta0), storage modulus (G') and loss modulus (G") increase than that of pure gel in the GNCs. The pure sol and the sols of gel nanocomposites (GNCs) exhibit Newtonian behavior for low shear rate (< 6 x 10(-3) s(-1)) and power law variation for the higher shear rate region. The characteristic time (A) increase with increasing clay concentration and the power law index (n) decreases with increase in clay concentration in the GNCs indicating increased shear thinning for the clay addition. Thus the sols of om-clay nanocomposites of PANI-DNNDSA system are easily processible. The storage modulus (G') of GNC sols are higher than that of pure PANI-DNNDSA sol, GNC1 sol shows a maximum of 733% increase in storage modulus and the percent increase decreases with increase in temperature. Exfoliated nature of clay tactoids has been attributed for the above dramatic increase of G'. The PANI-DNNDSA sol nanocomposites behave as a pseudo-solid at higher frequency where G' and loss modulus (G") show a crossover point in the frequency sweep experiment at a fixed temperature. The crossover frequency decreases with increase in clay concentration and it increases with increase in temperature for GNC sols. The pseudo-solid behavior has been explained from jamming or network formation of clay tactoids under shear. A probable explanation of the two apparently contradictory phenomena of shear thinning versus pseudo-solid behavior of the nanocomposite sols is discussed.

  10. Investigation of the charge boost technology for the efficiency increase of closed sorption thermal energy storage systems

    NASA Astrophysics Data System (ADS)

    Rohringer, C.; Engel, G.; Köll, R.; Wagner, W.; van Helden, W.

    2017-10-01

    The inclusion of solar thermal energy into energy systems requires storage possibilities to overcome the gap between supply and demand. Storage of thermal energy with closed sorption thermal energy systems has the advantage of low thermal losses and high energy density. However, the efficiency of these systems needs yet to be increased to become competitive on the market. In this paper, the so-called “charge boost technology” is developed and tested via experiments as a new concept for the efficiency increase of compact thermal energy storages. The main benefit of the charge boost technology is that it can reach a defined state of charge for sorption thermal energy storages at lower temperature levels than classic pure desorption processes. Experiments are conducted to provide a proof of principle for this concept. The results show that the charge boost technology does function as predicted and is a viable option for further improvement of sorption thermal energy storages. Subsequently, a new process application is developed by the author with strong focus on the utilization of the advantages of the charge boost technology over conventional desorption processes. After completion of the conceptual design, the theoretical calculations are validated via experiments.

  11. Invasion of a semi-arid shrubland by annual grasses increases autotrophic and heterotrophic soil respiration rates due to altered soil moisture and temperature patterns

    NASA Astrophysics Data System (ADS)

    Mauritz, M.; Hale, I.; Lipson, D.

    2010-12-01

    Shrub <-> grassland conversions are a globally occurring phenomenon altering habitat structure, quality and nutrient cycling. Grasses and shrubs differ in their above and belowground biomass allocation, root architecture, phenology, litter quality and quantity. Conversion affects soil microbial communities, soil moisture and temperature and carbon (C) allocation patterns. However, the effect of conversion on C storage is regionally variable and there is no consistent direction of change. In Southern California invasion by annual grasses is a major threat to native shrub communities and it has been proposed that grass invasion increases NPP and ecosystem C storage (Wolkovich et al, 2009). In order to better understand how this shrub <-> grassland conversion changes ecosystem C storage it is important to understand the partitioning of soil respiration into autotrophic and heterotrophic components. Respiration was measured in plots under shrubs and grasses from February when it was cold and wet to July when it was hot and dry, capturing seasonal transitions in temperature and water availability. Roots were excluded under shrubs and grasses with root exclusion cores to quantify heterotrophic respiration. Using total soil respiration (Rt) = autotrophic respiration (root) (Ra)+ heterotrophic respiration (microbial) (Rh) the components contributing to total soil respiration can be evaluated. Respiration, soil moisture and temperature were measured daily at four hour intervals using Licor 8100 automated chamber measurements. Throughout the measurement period, Rt under grasses exceeded Rt under shrubs. Higher Rt levels under grasses were mainly due to higher Ra in grasses rather than changes in Rh. On average grass Ra was almost double shrub Ra. Higher grass respiration levels are partially explained by differences in soil moisture and temperature between shrubs and grasses. Respiration rates responded similarly to seasonal transitions regardless of treatment although Ra had a much stronger seasonal response. Across all months changes in respiration rates are explained by changes in soil moisture. However, within wet periods respiration rates increase with temperature. From February to April the soil was wet and respiration levels gradually increased as day time soil temperatures increased. From April onwards absence of precipitation events and rising soil temperatures caused the soils to rapidly dry out. As a result Rt rates declined and gradually converged with Rh levels. As soils dried, grass Ra declined more gradually than shrub Ra. This was contrary to our expectation that shrub roots would respire longer into the dry season because they have deeper roots and can access water. The high late-season levels of respiration observed in the grass community are possibly due to the presence of invasive forbs which have deep tap roots and continue to grow after the grasses have senesced. Conversion from native shrubs to annual invasive grasses increased both Rt and Rh which indicates changes in plant C allocation and decomposition rates of soil C. The continued encroachment of grasses on shrubland has important implications for the future of C storage in this system.

  12. High Energy Storage Density and Impedance Response of PLZT2/95/5 Antiferroelectric Ceramics

    PubMed Central

    Li, Bi; Liu, Qiuxiang; Tang, Xingui; Zhang, Tianfu; Jiang, Yanping; Li, Wenhua; Luo, Jie

    2017-01-01

    (Pb0.97La0.02)(Zr0.95Ti0.05)O3 (PLZT2/95/5) ceramics were successfully prepared via a solid-state reaction route. The dielectric properties were investigated in the temperature region of 26–650 °C. The dielectric diffuse anomaly in the dielectric relaxation was found in the high temperature region of 600–650 °C with increasing the measuring frequency, which was related to the dynamic thermal process of ionized oxygen vacancies generated in the high temperature. Two phase transition points were detected during heating, which were found to coexist from 150 to 200 °C. Electric field induced ferroelectric to antiferroelectric phase transition behavior of the (Pb0.97La0.02)(Zr0.95Ti0.05)O3 ceramics was investigated in this work with an emphasis on energy storage properties. A recoverable energy-storage density of 0.83 J/cm3 and efficiency of 70% was obtained in (Pb0.97La0.02)(Zr0.95Ti0.05)O3 ceramics at 55 kV/cm. Based on these results, (Pb0.97La0.02)(Zr0.95Ti0.05)O3 ceramics with a large recoverable energy-storage density could be a potential candidate for the applications in high energy-storage density ceramic capacitors. PMID:28772503

  13. Development of a concentrating solar power system using fluidized-bed technology for thermal energy conversion and solid particles for thermal energy storage

    DOE PAGES

    Ma, Z.; Mehos, M.; Glatzmaier, G.; ...

    2015-05-01

    Concentrating solar power (CSP) is an effective way to convert solar energy into electricity with an economic energy-storage capability for grid-scale, dispatchable renewable power generation. However, CSP plants need to reduce costs to be competitive with other power generation methods. Two ways to reduce CSP cost are to increase solar-to-electric efficiency by supporting a high-efficiency power conversion system, and to use low-cost materials in the system. The current nitrate-based molten-salt systems have limited potential for cost reduction and improved power-conversion efficiency with high operating temperatures. Even with significant improvements in operating performance, these systems face challenges in satisfying the costmore » and performance targets. This paper introduces a novel CSP system with high-temperature capability that can be integrated into a high-efficiency CSP plant and that meets the low-cost, high-performance CSP targets. Unlike a conventional salt-based CSP plant, this design uses gas/solid, two-phase flow as the heat-transfer fluid (HTF); separated solid particles as storage media; and stable, inexpensive materials for the high-temperature receiver and energy storage containment. We highlight the economic and performance benefits of this innovative CSP system design, which has thermal energy storage capability for base-load power generation.« less

  14. Characteristics of phase-change materials containing oxide nano-additives for thermal storage

    PubMed Central

    2012-01-01

    In this study, the authors report the production of nanocomposite-enhanced phase-change materials (NEPCMs) using the direct-synthesis method by mixing paraffin with alumina (Al2O3), titania (TiO2), silica (SiO2), and zinc oxide (ZnO) as the experimental samples. Al2O3, TiO2, SiO2, and ZnO were dispersed into three concentrations of 1.0, 2.0, and 3.0 wt.%. Through heat conduction and differential scanning calorimeter experiments to evaluate the effects of varying concentrations of the nano-additives on the heat conduction performance and thermal storage characteristics of NEPCMs, their feasibility for use in thermal storage was determined. The experimental results demonstrate that TiO2 is more effective than the other additives in enhancing both the heat conduction and thermal storage performance of paraffin for most of the experimental parameters. Furthermore, TiO2 reduces the melting onset temperature and increases the solidification onset temperature of paraffin. This allows the phase-change heat to be applicable to a wider temperature range, and the highest decreased ratio of phase-change heat is only 0.46%, compared to that of paraffin. Therefore, this study demonstrates that TiO2, added to paraffin to form NEPCMs, has significant potential for enhancing the thermal storage characteristics of paraffin. PMID:23127224

  15. Physicochemical, rheological and structural characteristics of starch in maize tortillas.

    PubMed

    Hernández-Uribe, Juan P; Ramos-López, Gonzalo; Yee-Madeira, Hernani; Bello-Pérez, Luis A

    2010-06-01

    Fresh and stored maize (white and blue) tortillas were evaluated for physicochemical, rheological and structural characteristics assessed by calorimetry, x-ray diffraction, Fourier transform infrared (FTIR) spectroscopy, dynamic viscoelastic tests, and high-performance size-exclusion chromatography. Two endotherms were found in studies of fresh and stored tortillas. The low temperature endotherm (50-56 degrees C) was due to reorganized (retrograded) amylopectin, while the high temperature endotherm (105-123 degrees C) was attributed to retrograded amylose. The enthalpy value for the lower temperature transition was minor than that of the high temperature transition. Fresh tortillas showed an amorphous starch arrangement by x-ray diffraction study. Stored samples showed the presence of peaks at 2theta = 17 masculine and 23 masculine, indicating re-crystallization of starch components. FTIR results confirmed the development of higher levels of starch crystals during storage. Differences in the viscoelastic parameters were also observed between fresh and stored samples. At the longest storage times, white tortillas were more rigid than blue tortillas. Molar mass values for starch increased for both white and blue tortillas as storage time progressed, though relatively higher values were obtained for white tortillas. More starch reorganization occurred in white tortillas, in accordance to calorimetric, x-ray diffraction, FTIR and rheological results. These results corroborate that changes occurring in tortillas during storage are related to reorganization of starch components, and the maize variety more than the color plays an important role.

  16. Quality assessment of filtered smoked yellowfin tuna (Thunnus albacares) steaks.

    PubMed

    Pivarnik, Lori F; Faustman, Cameron; Rossi, Santiago; Suman, Surendranath P; Palmer, Catherine; Richard, Nicole L; Ellis, P Christopher; DiLiberti, Michael

    2011-08-01

    Filtered smoke (FS) has been used to preserve taste, texture, and/or color in tuna and other fish species. This treatment is particularly important in color preservation during frozen storage. The objective of this study was to compare changes in the quality profiles of FS-treated and untreated (UT) yellowfin tuna (Thunnus albacares) steaks stored in 3 ways: room temperature (21 to 22 °C), refrigerated (4 to 5 °C), and iced (0 °C). FS and UT steaks were processed from the same lot of fish and analyzed for chemical, microbiological, lipid oxidation, color, and sensory profiles. Similar trends were seen for microbial proliferation and accumulation of apparent ammonia and total volatile base nitrogen (TVB-N) during the storage temperatures evaluated. Notable exception in quality profile was found in lipid oxidation which was, as expected, lower for treated samples at all storage temperatures for TBARS (P < 0.05) and lower or significantly (P < 0.05) lower for POV values. FS increased the initial redness value significantly (P < 0.05). Unlike UT product, there was no loss of color value concomitant with quality changes for FS-treated tuna for all storage temperatures evaluated. The overall goal of this project was to evaluate filtered smoked tuna steaks as to the impact on the overall quality profile. As a color-stabilizing technology, it could mask deteriorating quality. © 2011 Institute of Food Technologists®

  17. Investigation of solid nitrogen for cryogenic thermal storage in superconducting cable terminations for enhanced resiliency

    NASA Astrophysics Data System (ADS)

    Suttell, N.; Zhang, Z.; Kweon, J.; Nes, T.; Kim, C. H.; Pamidi, S.; Ordonez, J. C.

    2017-12-01

    Low heat capacity of helium makes the helium gas cooled high temperature superconducting (HTS) power devices susceptible to large temperature rises during unexpected heat loads such as electrical faults or cryogenic system failures. Cryogenic thermal storage in the form of solid nitrogen designed in the terminations is explored as a means to increase the thermal stability and operational time of HTS power cables in the event of unexpected heat loads. An external tank containing activated charcoal is used as an adsorption buffer tank for nitrogen gas. The use of activated charcoal minimizes the volume of the buffer tank and prevents pressure rises during melting and boiling of the solid nitrogen. Calculations of the cryogenic thermal storage needed and a description of the experimental setup used to understand the design constraints are discussed.

  18. 40 CFR Table 4 to Subpart Ffff of... - Emission Limits for Storage Tanks

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... applies to your storage tanks: For each . . . For which . . . Then you must . . . 1. Group 1 storage tank a. The maximum true vapor pressure of total HAP at the storage temperature is ≥76.6 kilopascals i... maximum true vapor pressure of total HAP at the storage temperature is <76.6 kilopascals i. Comply with...

  19. 40 CFR Table 4 to Subpart Ffff of... - Emission Limits for Storage Tanks

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... applies to your storage tanks: For each . . . For which . . . Then you must . . . 1. Group 1 storage tank a. The maximum true vapor pressure of total HAP at the storage temperature is ≥76.6 kilopascals i... maximum true vapor pressure of total HAP at the storage temperature is <76.6 kilopascals i. Comply with...

  20. Effect of osmotic dehydration pretreatment and glassy state storage on the quality attributes of frozen mangoes under long-term storage.

    PubMed

    Zhao, Jin-Hong; Xiao, Hong-Wei; Ding, Yang; Nie, Ying; Zhang, Yu; Zhu, Zhen; Tang, Xuan-Ming

    2017-05-01

    Changes in the quality of frozen mango cuboids were investigated during long-term glassy state storage with and without osmotic dehydration pretreatment. The mango cuboids were dehydrated in mixed solutions (sucrose: glucose: fructose in a ratio of 3.6:1:3) of different concentrations (30, 40, and 50% (wt/wt)) prior to freezing and then stored at -55 °C (in the glassy state) for 6 months. The results revealed that compared with the untreated samples, osmotic pretreatment decreased total color difference (reduced by 15.6-62.3%), drip loss (reduced by 8.2-29.5%) and titration acidity (reduced by 1.3-9.4%), while increasing hardness (increased by 48.8-82.3%), vitamin C content (increased by 72.5-120.6%) and total soluble solids (increased by 21.8-53.7%) of frozen mangoes after 6 months. Dehydration with a sugar concentration of 40% was considered as the optimal pretreatment condition. In addition, a storage temperature of -55 °C provided better retention of quality than rubbery state storage at -18 °C. With prolonged storage time, the quality of frozen mangoes continued to change, even in the glassy state. However, the changes in quality of the osmotic-dehydrated samples were less than those of the untreated samples. The current work indicates that osmotic pretreatment and glassy state storage significantly improved the quality of frozen mangoes during long-term storage.

  1. Effect of sweetener combination and storage temperature on physicochemical properties of sucrose free white chocolate.

    PubMed

    Rodriguez Furlán, Laura T; Baracco, Yanina; Lecot, Javier; Zaritzky, Noemi; Campderrós, Mercedes E

    2017-08-15

    The influence of a combination of sweeteners (Stevia (St) and sucralose (Su)) and storage temperature on thermal properties, microstructure, water content, texture and Bloom of sucrose free white chocolate was investigated. A strong relationship between the microstructure and the highest percentage of Bloom was observed. The samples with 100%Su and 50%S+50%Su presented microstructures with channels through which solids and fat could more easily spread to the surface, increasing the fat and sugar Bloom formation. However, 50%St+50%Su and 75%St+25%Su samples showed a minimum Bloom formation, probably due to its dense microstructure with no void spaces. The differential scanning calorimetry studies demonstrated that the samples containing 100%St and 75%St+25%Su showed the smallest decrease of melting enthalpy with increasing temperature. Besides, non-isothermal crystallization kinetics was studied by applying Avrami model. The sample 75%St+25%Su presented the highest values of activation energy showing the greatest stability in the temperature range studied (7°C-30°C). Copyright © 2017 Elsevier Ltd. All rights reserved.

  2. Optimum Temperature for Storage of Fruit and Vegetables with Reference to Chilling Injury

    NASA Astrophysics Data System (ADS)

    Murata, Takao

    Cold storage is an important technique for preserving fresh fruit and vegetables. Deterioration due to ripening, senescence and microbiological disease can be retarded by storage at optimum temperature being slightly above the freezing point of tissues of fruit and vegetables. However, some fruit and vegetables having their origins in tropical or subtropical regions of the world are subject to chilling injury during transportation, storage and wholesale distribution at low temperature above freezing point, because they are usually sensitive to low temperature in the range of 15&digC to 0°C. This review will focus on the recent informations regarding chilling injury of fruit and vegetables, and summarize the optimum temperature for transportation and storage of fruit and vegetables in relation to chilling injury.

  3. 40 CFR 60.116b - Monitoring of operations.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... range. (e) Available data on the storage temperature may be used to determine the maximum true vapor...: (i) Available data on the Reid vapor pressure and the maximum expected storage temperature based on... Liquid Storage Vessels (Including Petroleum Liquid Storage Vessels) for Which Construction...

  4. Control of liquid cooling garments: technical control of body heat storage.

    PubMed

    Hexamer, M; Werner, J

    1996-07-01

    This paper describes a concept of how liquid cooling garments (LCG) can be automatically controlled by the objective physiological state. The technically controlled parameter was mean body temperature which was calculated from the measured rectal and mean skin temperature. This was motivated by the fact that mean body temperature is the basis for estimating body heat storage, a commonly used measure of thermal strain. Here the setpoint of mean body temperature was the individual value taken in a preceeding resting period and it was the task of the technical controller to keep the actual value of mean body temperature as close as possible to the setpoint. The most important tuning parameters of the controller were the weighting coefficients for rectal and mean skin temperature in the calculation for mean body temperature. The ratio of these two coefficients determined the degree of compensation for any rectal temperature shift by changing mean skin temperature. Test experiments were carried out (n = 5) in which the controller was able to clamp mean body temperature to the setpoint thereby preventing heat storage. Although exercise rate (75 W) was the same, sweating and warm discomfort occurred in some cases due to the individual rectal temperature rise. Another source of discomfort were delays or paradoxical time courses of rectal temperature at the start or end of exercise which were responsible for a delayed onset of cooling or heating. To avoid these effects, the oxygen consumption signal, which is very fast and directly correlated to the exercise rate, was added to the control loop. Each increase of this parameter above its resting level lowered suit temperature. As heat storage should not be completely rejected by this new signal pathway, the controller for mean body temperature still remained active. The repetition of the experiments showed that the load error in the control loop was smaller and the comfort level in transient phases higher. For a further improvement of this concept it is recommended that the weighting coefficients be tuned to the individual requirements.

  5. Moisture Sorption Behaviour and Mould Ecology of Trade Garri Sold in South Eastern Nigeria

    PubMed Central

    Samuel, Tochukwu; Ugwuanyi, J. Obeta

    2014-01-01

    Garri is a creamy white or yellow starchy grit produced by roasting to gelatinization and dryness of peeled, washed, mashed, and fermented dewatered cassava roots. It is the most important product of cassava in West and Central Africa. Mean moisture content of yellow and white garri was 11.11% and 10.81% within 24 hrs of sampling from the market, increasing to 17.27% and 16.14%, respectively, following 3 months of storage at room temperature. The water activity of samples varied from initial 0.587 to 0.934 following storage. Moisture sorption isotherms, determined by static gravimetric techniques at 20° and 30°C, showed temperature dependent BET Sigmoidal type II behaviour typical of carbohydrate rich foods but modulated very slightly by the content of palm oil. Equilibrium moisture content decreased with increase in temperature at constant water activity. A total of 10 fungal species belonging to the genera Mucor, Penicillium, Cephalosporium, Aspergillus, Scopulariopsis, Rhizopus, and Paecilomyces were identified, with range increasing with water activity of samples. PMID:26904621

  6. Experimental results from a laboratory-scale molten salt thermocline storage

    NASA Astrophysics Data System (ADS)

    Seubert, Bernhard; Müller, Ralf; Willert, Daniel; Fluri, Thomas

    2017-06-01

    Single-tank storage presents a valid option for cost reduction in thermal energy storage systems. For low-temperature systems with water as storage medium this concept is widely implemented and tested. For high-temperature systems very limited experimental data are publicly available. To improve this situation a molten salt loop for experimental testing of a single-tank storage prototype was designed and built at Fraunhofer ISE. The storage tank has a volume of 0.4 m3 or a maximum capacity of 72 kWhth. The maximum charging and discharging power is 60 kW, however, a bypass flow control system enables to operate the system also at a very low power. The prototype was designed to withstand temperatures up to 550 °C. A cascaded insulation with embedded heating cables can be used to reduce the effect of heat loss on the storage which is susceptible to edge effects due to its small size. During the first tests the operating temperatures were adapted to the conditions in systems with thermal oil as heat transfer fluid and a smaller temperature difference. A good separation between cold and hot fluid was achieved with temperature gradients of 95 K within 16 cm.

  7. Biogenic amine survey and organoleptic changes in fresh, stored, and temperature-abused bluefish (Pomatomus saltatrix).

    PubMed

    Gingerich, T M; Lorca, T; Flick, G J; Pierson, M D; McNair, H M

    1999-09-01

    Changes in histamine, putrescine, and cadaverine concentrations in bluefish filets (Pomatomus saltatrix) stored at 5, 10, and 15 degrees C were determined using high-performance liquid chromatography. An organoleptic assessment was conducted simultaneously with the biogenic amine analyses. The histamine levels found in fresh bluefish obtained from wholesale seafood distributors ranged between <1 ppm and 99 with an average of 39 ppm. Putrescine and cadaverine were not found in fresh bluefish. Fish fillets stored at each of the three temperatures developed histamine. The greatest accumulation of histamine was observed in fish stored at 15 degrees C, which developed histamine levels as high as 2,200 ppm. Putrescine levels increased at each temperature during storage. Cadaverine was present only in uninoculated bluefish stored at 15 degrees C. Histamine achieved higher levels in bluefish pieces inoculated with Morganella morganii, which demonstrates that bluefish support bacterial histamine formation. Histamine levels at each temperature exceeded the 50-ppm advisory level established by the Food and Drug Administration before 100% sensory rejection. Standard plate counts increased during storage of fish at all temperatures, but the correlation between histamine levels and standard plate count was not significant.

  8. Influence of body heat content on hand function during prolonged cold exposures.

    PubMed

    Flouris, A D; Cheung, S S; Fowles, J R; Kruisselbrink, L D; Westwood, D A; Carrillo, A E; Murphy, R J L

    2006-09-01

    We examined the influence of 1) prior increase [preheating (PHT)], 2) increase throughout [heating (HT)], and 3) no increase [control (Con)] of body heat content (H(b)) on neuromuscular function and manual dexterity of the hands during a 130-min exposure to -20 degrees C (coldEx). Ten volunteers randomly underwent three passive coldEx, incorporating a 10-min moderate-exercise period at the 65th min while wearing a liquid conditioning garment (LCG) and military arctic clothing. In PHT, 50 degrees C water was circulated in the LCG before coldEx until core temperature was increased by 0.5 degrees C. In HT, participants regulated the inlet LCG water temperature throughout coldEx to subjective comfort, while the LCG was not operating in Con. Thermal comfort, rectal temperature, mean skin temperature, mean finger temperature (T(fing)), change in H(b) (DeltaH(b)), rate of body heat storage, Purdue pegboard test, finger tapping, handgrip, maximum voluntary contraction, and evoked twitch force of the first dorsal interosseus muscle were recorded. Results demonstrated that, unlike in HT and PHT, thermal comfort, rectal temperature, mean skin temperature, twitch force, maximum voluntary contraction, and finger tapping declined significantly in Con. In contrast, T(fing) and Purdue pegboard test remained constant only in HT. Generalized estimating equations demonstrated that DeltaH(b) and T(fing) were associated over time with hand function, whereas no significant association was detected for rate of body heat storage. It is concluded that increasing H(b) not only throughout but also before a coldEx is effective in maintaining hand function. In addition, we found that the best indicator of hand function is DeltaH(b) followed by T(fing).

  9. Carbohydrate-Based Ice Recrystallization Inhibitors Increase Infectivity and Thermostability of Viral Vectors

    NASA Astrophysics Data System (ADS)

    Ghobadloo, Shahrokh M.; Balcerzak, Anna K.; Gargaun, Ana; Muharemagic, Darija; Mironov, Gleb G.; Capicciotti, Chantelle J.; Briard, Jennie G.; Ben, Robert N.; Berezovski, Maxim V.

    2014-07-01

    The inability of vaccines to retain sufficient thermostability has been an obstacle to global vaccination programs. To address this major limitation, we utilized carbohydrate-based ice recrystallization inhibitors (IRIs) to eliminate the cold chain and stabilize the potency of Vaccinia virus (VV), Vesicular Stomatitis virus (VSV) and Herpes virus-1 (HSV-1). The impact of these IRIs was tested on the potency of the viral vectors using a plaque forming unit assay following room temperature storage, cryopreservation with successive freeze-thaw cycles and lyophilization. Viral potency after storage with all three conditions demonstrated that N-octyl-gluconamide (NOGlc) recovered the infectivity of shelf stored VV, 5.6 Log10 PFU mL-1 during 40 days, and HSV-1, 2.7 Log10 PFU mL-1 during 9 days. Carbon-linked antifreeze glycoprotein analogue ornithine-glycine-glycine-galactose (OGG-Gal) increases the recovery of VV and VSV more than 1 Log10 PFU mL-1 after 10 freeze-thaw cycles. In VSV, cryostorage with OGG-Gal maintains high infectivity and reduces temperature-induced aggregation of viral particles by 2 times that of the control. In total, OGG-Gal and NOGlc preserve virus potency during cryostorage. Remarkably, NOGlc has potential to eliminate the cold chain and permit room temperature storage of viral vectors.

  10. Effect of reductive treatments on Pt behavior and NOx storage in lean NOx trap catalysts

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Wang, Xianqin; Kim, Do Heui; Kwak, Ja Hun

    2011-10-01

    Lean NOx trap (LNT) catalysts represent a promising approach to meet increasingly stringent NOx emission regulations on diesel and other lean-burn engines. Pt material properties, including dispersion and particle size, are known to be important factors in determining NOx uptake performance, since Pt provides active sites for NO oxidation to NO2 necessary for storing NOx as nitrates, and for the reduction of nitrates to N2. In this work, the physicochemical properties of Pt in Pt-BaO/Al2O3 LNT catalysts, such as the Pt accessible surface area and particle size, were investigated by using various tools, such as irreversible volumetric H2 chemisorption, highmore » resolution transmission electron microscopy (HRTEM), and X-ray diffraction (XRD), following successive reductive treatments at elevated temperatures. NOx uptake activities were also measured to establish a relationship between the properties of Pt and NOx storage following identical high-temperature reductive treatments. We find that the reductive treatments of Pt-BaO/Al2O3 lean NOx trap catalysts at temperatures up to 500 ºC promote a significant increase in NOx uptake explained, in part, by an induced close interaction between Pt and BaO phases in the catalyst, thus enabling facilitation of the NOx storage process.« less

  11. Carbohydrate-Based Ice Recrystallization Inhibitors Increase Infectivity and Thermostability of Viral Vectors

    PubMed Central

    Ghobadloo, Shahrokh M.; Balcerzak, Anna K.; Gargaun, Ana; Muharemagic, Darija; Mironov, Gleb G.; Capicciotti, Chantelle J.; Briard, Jennie G.; Ben, Robert N.; Berezovski, Maxim V.

    2014-01-01

    The inability of vaccines to retain sufficient thermostability has been an obstacle to global vaccination programs. To address this major limitation, we utilized carbohydrate-based ice recrystallization inhibitors (IRIs) to eliminate the cold chain and stabilize the potency of Vaccinia virus (VV), Vesicular Stomatitis virus (VSV) and Herpes virus-1 (HSV-1). The impact of these IRIs was tested on the potency of the viral vectors using a plaque forming unit assay following room temperature storage, cryopreservation with successive freeze-thaw cycles and lyophilization. Viral potency after storage with all three conditions demonstrated that N-octyl-gluconamide (NOGlc) recovered the infectivity of shelf stored VV, 5.6 Log10 PFU mL−1 during 40 days, and HSV-1, 2.7 Log10 PFU mL−1 during 9 days. Carbon-linked antifreeze glycoprotein analogue ornithine-glycine-glycine-galactose (OGG-Gal) increases the recovery of VV and VSV more than 1 Log10 PFU mL−1 after 10 freeze-thaw cycles. In VSV, cryostorage with OGG-Gal maintains high infectivity and reduces temperature-induced aggregation of viral particles by 2 times that of the control. In total, OGG-Gal and NOGlc preserve virus potency during cryostorage. Remarkably, NOGlc has potential to eliminate the cold chain and permit room temperature storage of viral vectors. PMID:25078058

  12. Thermophysical Characterization of MgCl₂·6H₂O, Xylitol and Erythritol as Phase Change Materials (PCM) for Latent Heat Thermal Energy Storage (LHTES).

    PubMed

    Höhlein, Stephan; König-Haagen, Andreas; Brüggemann, Dieter

    2017-04-24

    The application range of existing real scale mobile thermal storage units with phase change materials (PCM) is restricted by the low phase change temperature of 58 ∘ C for sodium acetate trihydrate, which is a commonly used storage material. Therefore, only low temperature heat sinks like swimming pools or greenhouses can be supplied. With increasing phase change temperatures, more applications like domestic heating or industrial process heat could be operated. The aim of this study is to find alternative PCM with phase change temperatures between 90 and 150 ∘ C . Temperature dependent thermophysical properties like phase change temperatures and enthalpies, densities and thermal diffusivities are measured for the technical grade purity materials xylitol (C 5 H 12 O 5 ), erythritol (C 4 H 10 O 4 ) and magnesiumchloride hexahydrate (MCHH, MgCl 2 · 6H 2 O). The sugar alcohols xylitol and erythritol indicate a large supercooling and different melting regimes. The salt hydrate MgCl 2 · 6H 2 O seems to be a suitable candidate for practical applications. It has a melting temperature of 115.1 ± 0.1 ∘ C and a phase change enthalpy of 166.9 ± 1.2 J / g with only 2.8 K supercooling at sample sizes of 100 g . The PCM is stable over 500 repeated melting and solidification cycles at differential scanning calorimeter (DSC) scale with only small changes of the melting enthalpy and temperature.

  13. Debranching and temperature-cycled crystallization of waxy rice starch and their digestibility.

    PubMed

    Zeng, Feng; Ma, Fei; Gao, Qunyu; Yu, Shujuan; Kong, Fansheng; Zhu, Siming

    2014-11-26

    Slowly digestible starch (SDS) was obtained through debranched waxy rice starch and subsequent crystallization under isothermal and temperature-cycled conditions. Temperature-cycled crystallization of dual 4/-20 °C produced a higher yield of SDS product than isotherm crystallization. Crystal structure of SDS products changed from A-type to a mixture of B and V-type X-ray diffraction patterns. The relative crystallinity was higher in the temperature-cycled samples than that of isotherm. Attenuated total reflectance Fourier transform infrared spectroscopy suggested that the peripheral regions of isothermal storage starch were better organized than temperature-cycles. Temperature cycling induced higher onset temperature for melting of crystals than isothermal storage under a differential scanning calorimeter. The cycled temperature storage induced a greater amount of SDS than the isothermal storage. Copyright © 2014 Elsevier Ltd. All rights reserved.

  14. Kinetic Behavior of Escherichia coli on Various Cheeses under Constant and Dynamic Temperature.

    PubMed

    Kim, K; Lee, H; Gwak, E; Yoon, Y

    2014-07-01

    In this study, we developed kinetic models to predict the growth of pathogenic Escherichia coli on cheeses during storage at constant and changing temperatures. A five-strain mixture of pathogenic E. coli was inoculated onto natural cheeses (Brie and Camembert) and processed cheeses (sliced Mozzarella and sliced Cheddar) at 3 to 4 log CFU/g. The inoculated cheeses were stored at 4, 10, 15, 25, and 30°C for 1 to 320 h, with a different storage time being used for each temperature. Total bacteria and E. coli cells were enumerated on tryptic soy agar and MacConkey sorbitol agar, respectively. E. coli growth data were fitted to the Baranyi model to calculate the maximum specific growth rate (μ max; log CFU/g/h), lag phase duration (LPD; h), lower asymptote (log CFU/g), and upper asymptote (log CFU/g). The kinetic parameters were then analyzed as a function of storage temperature, using the square root model, polynomial equation, and linear equation. A dynamic model was also developed for varying temperature. The model performance was evaluated against observed data, and the root mean square error (RMSE) was calculated. At 4°C, E. coli cell growth was not observed on any cheese. However, E. coli growth was observed at 10°C to 30°C with a μ max of 0.01 to 1.03 log CFU/g/h, depending on the cheese. The μ max values increased as temperature increased, while LPD values decreased, and μ max and LPD values were different among the four types of cheese. The developed models showed adequate performance (RMSE = 0.176-0.337), indicating that these models should be useful for describing the growth kinetics of E. coli on various cheeses.

  15. Thermophysical and Mechanical Properties of Hardened Cement Paste with Microencapsulated Phase Change Materials for Energy Storage

    PubMed Central

    Cui, Hongzhi; Liao, Wenyu; Memon, Shazim Ali; Dong, Biqin; Tang, Waiching

    2014-01-01

    In this research, structural-functional integrated cement-based materials were prepared by employing cement paste and a microencapsulated phase change material (MPCM) manufactured using urea-formaldehyde resin as the shell and paraffin as the core material. The encapsulation ratio of the MPCM could reach up to 91.21 wt%. Thermal energy storage cement pastes (TESCPs) incorporated with different MPCM contents (5%, 10%, 15%, 20% and 25% by weight of cement) were developed, and their thermal and mechanical properties were studied. The results showed that the total energy storage capacity of the hardened cement specimens with MPCM increased by up to 3.9-times compared with that of the control cement paste. The thermal conductivity at different temperature levels (35–36 °C, 55–56 °C and 72–74 °C) decreased with the increase of MPCM content, and the decrease was the highest when the temperature level was 55–56 °C. Moreover, the compressive strength, flexural strength and density of hardened cement paste decreased with the increase in MPCM content linearly. Among the evaluated properties, the compressive strength of TESCPs had a larger and faster degradation with the increase of MPCM content. PMID:28788291

  16. Temperature Affects the Use of Storage Fatty Acids as Energy Source in a Benthic Copepod (Platychelipus littoralis, Harpacticoida).

    PubMed

    Werbrouck, Eva; Van Gansbeke, Dirk; Vanreusel, Ann; De Troch, Marleen

    2016-01-01

    The utilization of storage lipids and their associated fatty acids (FA) is an important means for organisms to cope with periods of food shortage, however, little is known about the dynamics and FA mobilization in benthic copepods (order Harpacticoida). Furthermore, lipid depletion and FA mobilization may depend on the ambient temperature. Therefore, we subjected the temperate copepod Platychelipus littoralis to several intervals (3, 6 and 14 days) of food deprivation, under two temperatures in the range of the normal habitat temperature (4, 15 °C) and under an elevated temperature (24 °C), and studied the changes in FA composition of storage and membrane lipids. Although bulk depletion of storage FA occurred after a few days of food deprivation under 4 °C and 15 °C, copepod survival remained high during the experiment, suggesting the catabolization of other energy sources. Ambient temperature affected both the degree of FA depletion and the FA mobilization. In particular, storage FA were more exhausted and FA mobilization was more selective under 15 °C compared with 4 °C. In contrast, depletion of storage FA was limited under an elevated temperature, potentially due to a switch to partial anaerobiosis. Food deprivation induced selective DHA retention in the copepod's membrane, under all temperatures. However, prolonged exposure to heat and nutritional stress eventually depleted DHA in the membranes, and potentially induced high copepod mortality. Storage lipids clearly played an important role in the short-term response of the copepod P. littoralis to food deprivation. However, under elevated temperature, the use of storage FA as an energy source is compromised.

  17. Factors influencing survival of mammalian cells exposed to hypothermia. VI. Effects of prehypothermic hypoxia followed by aerobic or hypoxic storage at various hypothermic temperatures.

    PubMed

    Kruuv, J; Lepock, J R

    1995-04-01

    The Arrhenius plot of inactivation (killing) rates of V-79 Chinese hamster cells exposed to hypothermia in air-equilibrated (aerobic) medium contains a break at about 8 degrees C, which corresponds to the minimum inactivation rate, implying that there are distinct hypothermic damage mechanisms above (range I, 8 to 25 degrees C) and below (range II, 0 to 8 degrees C) 8 degrees C. Prehypothermic hypoxia (PHH) for 75 min at room temperature sensitizes cells to subsequent aerobic hypothermia at both 5 and 10 degrees C (range II and I). However, PHH followed by severe hypoxia (0.03 microM oxygen in the medium) protected cells during 10 degrees C (range I) storage by increasing the shoulder, but not the slope, of the cell survival curve compared to the PHH plus 10 degrees C aerobic hypothermia case. On the other hand, PHH plus severe hypoxia during 5 degrees C storage (range II) protected cells by decreasing the slope, but not the shoulder, of the cell survival curve compared to the PHH plus 5 degrees C aerobic hypothermia case. Furthermore, PHH plus severe hypoxia during 5 degrees C storage was not significantly worse than aerobic storage without PHH at 5 degrees C. With or without severe hypoxia, 10 degrees C storage is preferable to 5 degrees C storage in this cell line. Extrapolated to organ storage, the results may imply that if warm ischemia (PHH) has occurred, subsequent hypoxic hypothermic perfusion storage may be preferable to aerobic hypothermic perfusion storage.

  18. Storage mite contamination of commercial dry dog food in south-eastern Australia.

    PubMed

    Hibberson, C E; Vogelnest, L J

    2014-06-01

    To evaluate contamination of unopened and opened stored sources of commercial dry dog food by viable storage mites. Prospective laboratory and field study. Samples were collected from nine brands of previously unopened bags (new bags) of dry food and 20 field sources of stored dry food in homes in Sydney and Canberra, Australia. All samples were initially examined for the presence of mites using a stereo-binocular microscope and then placed in separate filter-paper-sealed containers. Field samples were incubated at an average temperature of 29°C and 78% relative humidity (RH) for 5 weeks and then at average 26°C/83% RH for 8 weeks. Paired new-bag samples were stored under room conditions (average 23°C/47% RH) and controlled incubator conditions (average 26°C/80% RH) for 6 weeks. All samples were thoroughly examined for mites, mite eggs and visible mould once weekly using a stereo-binocular microscope. Storage mites were not visualised in any of the field samples or in new-bag samples stored at room temperature. Storage mites, identified as Tyrophagus putrescentiae, were visualised in increasing numbers in seven of nine new-bag samples after incubation, with first mites and then eggs evident after 3 weeks of incubation. We confirmed the presence of viable storage mites in a range of previously unopened commercial dry dog foods in Australia and confirmed the possibility of heavy storage mite contamination for dry food stored under conditions of moderate temperature and high humidity. These findings have relevance to storage mite and/or dust mite sensitivity in canine atopic dermatitis. © 2014 Australian Veterinary Association.

  19. Label-free quantitative proteomics to investigate strawberry fruit proteome changes under controlled atmosphere and low temperature storage.

    PubMed

    Li, Li; Luo, Zisheng; Huang, Xinhong; Zhang, Lu; Zhao, Pengyu; Ma, Hongyuan; Li, Xihong; Ban, Zhaojun; Liu, Xia

    2015-04-29

    To elucidate the mechanisms contributing to fruit responses to senescence and stressful environmental stimuli under low temperature (LT) and controlled atmosphere (CA) storage, a label-free quantitative proteomic investigation was conducted in strawberry (Fragaria ananassa, Duch. cv. 'Akihime'). Postharvest physiological quality traits including firmness, total soluble solids, total acidity, ascorbic acid and volatile production were characterized following storage under different conditions. The observed post-storage protein expression profiles may be associated with delayed senescence features in strawberry. A total of 454 proteins were identified in differentially treated strawberry fruits. Quantitative analysis, using normalized spectral counts, revealed 73 proteins common to all treatments, which formed three clusters in a hierarchical clustering analysis. The proteins spanned a range of functions in various metabolic pathways and networks involved in carbohydrate and energy metabolism, volatile biosynthesis, phenylpropanoid activity, stress response and protein synthesis, degradation and folding. After CA and LT storage, 16 (13) and 11 (17) proteins, respectively, were significantly increased (decreased) in abundance, while expression profile of 12 proteins was significantly changed by both CA and LT. To summarize, the differential variability of abundance in strawberry proteome, working in a cooperative manner, provided an overview of the biological processes that occurred during CA and LT storage. Controlled atmosphere storage at an optimal temperature is regarded to be an effective postharvest technology to delay fruit senescence and maintain fruit quality during shelf life. Nonetheless, little information on fruit proteomic changes under controlled atmosphere and/or low temperature storage is available. The significance of this paper is that it is the first study employing a label-free approach in the investigation of strawberry fruit response to controlled atmosphere and cold storage. Changes in postharvest physiological quality traits including volatile production, firmness, ascorbic acid, soluble solids and total acidity were also characterized. Significant biological changes associated with senescence were revealed and differentially abundant proteins under various storage conditions were identified. Proteomic profiles were linked to physiological aspects of strawberry fruit senescence in order to provide new insights into possible regulation mechanisms. Findings from this study not only provide proteomic information on fruit regulation, but also pave the way for further quantitative studies at the transcriptomic and metabolomic levels. Copyright © 2015 Elsevier B.V. All rights reserved.

  20. Dynamic mechanical analysis of multi-walled carbon nanotube/HDPE composites.

    PubMed

    Kanagaraj, S; Guedes, R M; Oliveira, Mónica S A; Simões, José A O

    2008-08-01

    Since the discovery of carbon nanotubes (CNTs), their remarkable properties make them ideal candidates to reinforce in advanced composites. In this attempt, an enhancement of mechanical properties of high density polyethylene (HDPE) by adding 1 wt% of CNTs is studied using Dynamic mechanical and Thermal analyzer (DMTA). The chemically treated and functionalized CNTs were homogeneously dispersed with HDPE and the test samples were made using injection molding machine. Using DMTA, storage modulus (E'), loss modulus (E") and damping factor (tan delta) of the sample under oscillating load were studied as a function of frequency of oscillation and temperatures. The storage modulus decreases with an increase of temperature and increases by adding CNTs in the composites where the reinforcing effect of CNT is confirmed. It is concluded that the large scale polymer relaxations in the composites are effectively restrained by the presence of CNTs and thus the mechanical properties of nanocomposites increase. The transition frequency of loss modulus is observed at 1 Hz. The loss modulus decreases with an increase of temperature at below 1 Hz but opposite trend was observed at above 1 Hz. The shift factor could be predicted from Williams-Landel-Ferry (WLF) model which has good agreement with experimental results.

  1. Using Encapsulated Phase Change Material in Thermal Energy Storage for Baseload Concentrating Solar Power (EPCM-TES)

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Mathur, Anoop

    2013-12-15

    Terrafore successfully demonstrated and optimized the manufacturing of capsules containing phase-changing inorganic salts. The phase change was used to store thermal energy collected from a concentrating solar-power plant as latent heat. This latent heat, in addition to sensible heat increased the energy density (energy stored per unit weight of salt) by over 50%, thus requiring 40% less salt and over 60% less capsule container. Therefore, the cost to store high-temperature thermal energy collected in a concentrating solar power plant will be reduced by almost 40% or more, as compared to conventional two-tank, sensible-only storage systems. The cost for thermal energymore » storage (TES) system is expected to achieve the Sun Shot goal of $15 per kWh(t). Costs associated with poor heat-transfer in phase change materials (PCM) were also eliminated. Although thermal energy storage that relies on the latent heat of fusion of PCM improves energy density by as much as 50%, upon energy discharge the salt freezes and builds on the heat transfer surfaces. Since these salts have low thermal conductivity, large heat-transfer areas, or larger conventional heat-exchangers are needed, which increases costs. By encapsulating PCM in small capsules we have increased the heat transfer area per unit volume of salt and brought the heat transfer fluid in direct contact with the capsules. These two improvements have increased the heat transfer coefficient and boosted heat transfer. The program was successful in overcoming the phenomenon of melt expansion in the capsules, which requires the creation of open volume in the capsules or shell to allow for expansion of the molten salt on melting and is heated above its melting point to 550°C. Under contract with the Department of Energy, Terrafore Inc. and Southwest Research Institute, developed innovative method(s) to economically create the open volume or void in the capsule. One method consists of using a sacrificial polymer coating as the middle layer between the salt prill and the shell material. The selected polymer decomposes at temperatures below the melting point of the salt and forms gases which escape through the pores in the capsule shell thus leaving a void in the capsule. We have demonstrated the process with a commonly used inorganic nitrate salt in a low-cost shell material that can withstand over 10,000 high-temperature thermal cycles, or a thirty-year or greater life in a solar plant. The shell used to encapsulate the salt was demonstrated to be compatible with molten salt heat transfer fluid typically used in CSP plants to temperatures up to 600 °C. The above findings have led to the concept of a cascaded arrangement. Salts with different melting points can be encapsulated using the same recipe and contained in a packed bed by cascading the salt melting at higher melting point at the top over the salt melting at lower melting point towards the bottom of the tank. This cascaded energy storage is required to effectively transfer the sensible heat collected in heat transfer fluids between the operating temperatures and utilize the latent heat of fusion in the salts inside the capsule. Mathematical models indicate that over 90% of the salts will undergo phase change by using three salts in equal proportion. The salts are selected such that the salt at the top of the tank melts at about 15°C below the high operating-temperature, and the salt at the bottom of the tank melts 15°C above the low operating-temperature. The salt in the middle of tank melts in-between the operating temperature of the heat transfer fluid. A cascaded arrangement leads to the capture of 90% of the latent-heat of fusion of salts and their sensible heats. Thus the energy density is increased by over 50% from a sensible-only, two-tank thermal energy storage. Furthermore, the Terrafore cascaded storage method requires only one tank as opposed to the two-tanks used in sensible heat storage. Since heat is transferred from the heat transfer fluid by direct contact with capsules, external heat-exchangers are not required for charging storage. Thus, the cost of the thermal storage system is reduced due to smaller containers and less salt. The optimum salt proportions, their melting temperature and the number of salts in the cascade are determined by raw materials costs and the mathematical model. We estimate the processing cost of the encapsulation to be low, where the major cost of the capsule will be the cost of the phase-change salt(s). Our economic analyses show that the cost of EPCM-TES is about $17.98 per kWh(t), which is about 40% lower than the $28.36 per kWh(t) for a two-tank sensible heat TES for a large scale CSP-TES design. Finally, additional improvements in the heat-transfer fluids, currently in development elsewhere will further improve the energy density to achieve the SunShot goal of $15 per kWh(t).« less

  2. SSH2S: Hydrogen storage in complex hydrides for an auxiliary power unit based on high temperature proton exchange membrane fuel cells

    NASA Astrophysics Data System (ADS)

    Baricco, Marcello; Bang, Mads; Fichtner, Maximilian; Hauback, Bjorn; Linder, Marc; Luetto, Carlo; Moretto, Pietro; Sgroi, Mauro

    2017-02-01

    The main objective of the SSH2S (Fuel Cell Coupled Solid State Hydrogen Storage Tank) project was to develop a solid state hydrogen storage tank based on complex hydrides and to fully integrate it with a High Temperature Proton Exchange Membrane (HT-PEM) fuel cell stack. A mixed lithium amide/magnesium hydride system was used as the main storage material for the tank, due to its high gravimetric storage capacity and relatively low hydrogen desorption temperature. The mixed lithium amide/magnesium hydride system was coupled with a standard intermetallic compound to take advantage of its capability to release hydrogen at ambient temperature and to ensure a fast start-up of the system. The hydrogen storage tank was designed to feed a 1 kW HT-PEM stack for 2 h to be used for an Auxiliary Power Unit (APU). A full thermal integration was possible thanks to the high operation temperature of the fuel cell and to the relative low temperature (170 °C) for hydrogen release from the mixed lithium amide/magnesium hydride system.

  3. Sensitivity of global and regional terrestrial carbon storage to the direct CO2 effect and climate change based on the CMIP5 model intercomparison.

    PubMed

    Peng, Jing; Dan, Li; Huang, Mei

    2014-01-01

    Global and regional land carbon storage has been significantly affected by increasing atmospheric CO2 concentration and climate change. Based on fully coupled climate-carbon-cycle simulations from the Coupled Model Intercomparison Project Phase 5 (CMIP5), we investigate sensitivities of land carbon storage to rising atmospheric CO2 concentration and climate change over the world and 21 regions during the 130 years. Overall, the simulations suggest that consistently spatial positive effects of the increasing CO2 concentrations on land carbon storage are expressed with a multi-model averaged value of 1.04 PgC per ppm. The stronger positive values are mainly located in the broad areas of temperate and tropical forest, especially in Amazon basin and western Africa. However, large heterogeneity distributed for sensitivities of land carbon storage to climate change. Climate change causes decrease in land carbon storage in most tropics and the Southern Hemisphere. In these regions, decrease in soil moisture (MRSO) and enhanced drought somewhat contribute to such a decrease accompanied with rising temperature. Conversely, an increase in land carbon storage has been observed in high latitude and altitude regions (e.g., northern Asia and Tibet). The model simulations also suggest that global negative impacts of climate change on land carbon storage are predominantly attributed to decrease in land carbon storage in tropics. Although current warming can lead to an increase in land storage of high latitudes of Northern Hemisphere due to elevated vegetation growth, a risk of exacerbated future climate change may be induced due to release of carbon from tropics.

  4. Sensitivity of Global and Regional Terrestrial Carbon Storage to the Direct CO2 Effect and Climate Change Based on the CMIP5 Model Intercomparison

    PubMed Central

    Peng, Jing; Dan, Li; Huang, Mei

    2014-01-01

    Global and regional land carbon storage has been significantly affected by increasing atmospheric CO2 concentration and climate change. Based on fully coupled climate-carbon-cycle simulations from the Coupled Model Intercomparison Project Phase 5 (CMIP5), we investigate sensitivities of land carbon storage to rising atmospheric CO2 concentration and climate change over the world and 21 regions during the 130 years. Overall, the simulations suggest that consistently spatial positive effects of the increasing CO2 concentrations on land carbon storage are expressed with a multi-model averaged value of 1.04PgC per ppm. The stronger positive values are mainly located in the broad areas of temperate and tropical forest, especially in Amazon basin and western Africa. However, large heterogeneity distributed for sensitivities of land carbon storage to climate change. Climate change causes decrease in land carbon storage in most tropics and the Southern Hemisphere. In these regions, decrease in soil moisture (MRSO) and enhanced drought somewhat contribute to such a decrease accompanied with rising temperature. Conversely, an increase in land carbon storage has been observed in high latitude and altitude regions (e.g., northern Asia and Tibet). The model simulations also suggest that global negative impacts of climate change on land carbon storage are predominantly attributed to decrease in land carbon storage in tropics. Although current warming can lead to an increase in land storage of high latitudes of Northern Hemisphere due to elevated vegetation growth, a risk of exacerbated future climate change may be induced due to release of carbon from tropics. PMID:24748331

  5. Enhancing electrical energy storage capability of dielectric polymer nanocomposites via the room temperature Coulomb blockade effect of ultra-small platinum nanoparticles.

    PubMed

    Wang, Liwei; Huang, Xingyi; Zhu, Yingke; Jiang, Pingkai

    2018-02-14

    Introducing a high dielectric constant (high-k) nanofiller into a dielectric polymer is the most common way to achieve flexible nanocomposites for electrostatic energy storage devices. However, the significant decrease of breakdown strength and large increase of dielectric loss has long been known as the bottleneck restricting the enhancement of practical energy storage capability of the nanocomposites. In this study, by introducing ultra-small platinum (<2 nm) nanoparticles, high-k polymer nanocomposites with high breakdown strength and low dielectric loss were prepared successfully. Core-shell structured polydopamine@BaTiO 3 (PDA@BT) and core-satellite ultra-small platinum decorated PDA@BT (Pt@PDA@BT) were used as nanofillers. Compared with PDA@BT nanocomposites, the maximum discharged energy density of the Pt@PDA@BT nanocomposites is increased by nearly 70% because of the improved energy storage efficiency. This research provides a simple, promising and unique way to enhance energy storage capability of high-k polymer nanocomposites.

  6. Development of a novel ultrasonic temperature probe for long-term monitoring of dry cask storage systems

    NASA Astrophysics Data System (ADS)

    Bakhtiari, S.; Wang, K.; Elmer, T. W.; Koehl, E.; Raptis, A. C.

    2013-01-01

    With the recent cancellation of the Yucca Mountain repository and the limited availability of wet storage utilities for spent nuclear fuel (SNF), more attention has been directed toward dry cask storage systems (DCSSs) for long-term storage of SNF. Consequently, more stringent guidelines have been issued for the aging management of dry storage facilities that necessitate monitoring of the conditions of DCSSs. Continuous health monitoring of DCSSs based on temperature variations is one viable method for assessing the integrity of the system. In the present work, a novel ultrasonic temperature probe (UTP) is being tested for long-term online temperature monitoring of DCSSs. Its performance was evaluated and compared with type N thermocouple (NTC) and resistance temperature detector (RTD) using a small-scale dry storage canister mockup. Our preliminary results demonstrate that the UTP system developed at Argonne is able to achieve better than 0.8 °C accuracy, tested at temperatures of up to 400 °C. The temperature resolution is limited only by the sampling rate of the current system. The flexibility of the probe allows conforming to complex geometries thus making the sensor particularly suited to measurement scenarios where access is limited.

  7. Effectiveness of a web-based education program to improve vaccine storage conditions in primary care (Keep Cool): study protocol for a randomized controlled trial.

    PubMed

    Thielmann, Anika; Viehmann, Anja; Weltermann, Birgitta M

    2015-07-14

    Immunization programs are among the most effective public health strategies worldwide. Adequate vaccine storage is a prerequisite to assure the vaccines' effectiveness and safety. In a questionnaire survey among a random sample of German primary care physicians, we discovered vaccine storage deficits: 16% of physicians had experience with cold chain breaches either as an error or near error, 49 % did not keep a temperature log, and 21 % did not use a separate refrigerator for vaccine storage. In a recent feasibility study of 21 practice refrigerators, we showed that these were outside the target range 10.2% of the total time with some single refrigerators being outside the target range as much as 66.3% of the time. These cooling-chain deficits are consistent with the international medical literature, yet an effective, easy to disseminate, practice-centered intervention to improve storage conditions is lacking. This randomized intervention trial will be conducted in a random sample of primary care practices. Based on continuous temperature recordings over 7 days, all practices with readings outside the target range for vaccine storage (+2 °C to +8 °C) will be randomly allocated to a web-based education program or a waiting list control group. The practice physicians and their teams constitute the target population. Participants will be educated about best practices in vaccine storage and will receive a manual including storage checklists and templates for temperature documentation. In all practices, temperatures of the vaccine refrigerators will be monitored continuously using a data logger with a glycol probe as a surrogate for vaccine vial temperature. The effectiveness of the web-based education program will be determined after 6 months in terms of the proportion of refrigerators with vaccine vial temperatures within the target range (+2 °C to +8 °C) during 7-day temperature logging. Secondary outcome parameters include temperature monitoring, no critically low temperatures (≤ -0.5 °C), compliance with storage recommendations, knowledge of good vaccine storage conditions, and assignment of personnel as vaccine storage manager and backup. Keep Cool will develop and evaluate a web-based education program to improve vaccine storage conditions in primary care and thereby ensure immunization safety and effectiveness. DRKS00006561 (date of registration: 20 February 2015).

  8. Temperature impact on the trophic transfer of fatty acids in the congeneric copepods Acartia tonsa and Acartia clausi

    NASA Astrophysics Data System (ADS)

    Werbrouck, Eva; Tiselius, Peter; Van Gansbeke, Dirk; Cervin, Gunnar; Vanreusel, Ann; De Troch, Marleen

    2016-06-01

    Copepods of the genus Acartia occur worldwide and constitute an important link to higher trophic levels in estuaries. However, biogeographical shifts in copepod assemblages and colonization of certain European estuaries by the invader A. tonsa, both driven or enhanced by increasing ocean temperature, raise the pressure on autochthonous copepod communities. Despite the profound effect of temperature on all levels of biological organization, its impact on the fatty acid (FA) dynamics of Acartia species is understudied. As certain FAs exert a bottom-up control on the trophic structure of aquatic ecosystems, temperature-induced changes in FA dynamics of Acartia species may impact higher trophic levels. Therefore, this study documents the short-term temperature responses of A. tonsa and A. clausi, characterized by their warm- versus cold-water preference respectively, by analyzing the FA profiles of their membrane and storage lipids under 5 and 15 °C. Copepods that were fed an ad libitum diet of the diatom Thalassiosira weissflogii (bloom conditions) under 15 °C increased their storage FA content substantially. Furthermore, the membrane FA composition of A. tonsa showed a more profound temperature response compared with A. clausi which might be linked with the eurythermal character of the former.

  9. Effect of gamma irradiation and storage time on microbial growth and physicochemical characteristics of pumpkin (Cucurbita Moschata Duchesne ex Poiret) puree.

    PubMed

    Gliemmo, María F; Latorre, María E; Narvaiz, Patricia; Campos, Carmen A; Gerschenson, Lía N

    2014-01-01

    The effect of gamma irradiation (0-2 kGy) and storage time (0-28 days) on microbial growth and physicochemical characteristics of a packed pumpkin puree was studied. For that purpose, a factorial design was applied. The puree contained potassium sorbate, glucose and vanillin was stored at 25°C . Gamma irradiation diminished and storage time increased microbial growth. A synergistic effect between both variables on microbial growth was observed. Storage time decreased pH and color of purees. Sorbate content decreased with storage time and gamma irradiation. Mathematical models of microbial growth generated by the factorial design allowed estimating that a puree absorbing 1.63 kGy would have a shelf-life of 4 days. In order to improve this time, some changes in the applied hurdles were assayed. These included a thermal treatment before irradiation, a reduction of irradiation dose to 0.75 kGy and a decrease in storage temperature at 20°C . As a result, the shelf-life of purees increased to 28 days.

  10. Storage changes in the quality of sound and sprouted flour.

    PubMed

    Sur, R; Nagi, H P; Sharma, S; Sekhon, K S

    1993-07-01

    Sound and sprouted flours (24 and 48 hr) from bread wheat (WL-1562), durum wheat (PBW-34) and triticale (TL-1210) were stored at room temperature (34.8 degrees C) and relative humidity (66.7%) for 0, 45, 90 and 135 days to assess the changes in physico-chemical and baking properties. Protein, gluten, sedimentation value, starch and crude fat decreased during storage in all the samples; however, the decrease was more in sprouted flours. Free amino acids, proteolytic activity, diastatic activity and damaged starch decreased with increase in storage period. Total sugars and free fatty acids increased more rapidly in the flours of sprouted wheats during 135 days of storage. Loaf volume of breads decreased during storage in both sound and sprouted flour but the mean percent decrease in loaf volume was more in stored sound flours. Aging of sprouted flour for 45 days improved the cookie and cake making properties but further storage was of no value for these baked products. Chapati making properties of stored sound and sprouted flour were inferior to that of fresh counterparts.

  11. Die another day: Fate of heat-treated Geobacillus stearothermophilus ATCC 12980 spores during storage under growth-preventing conditions.

    PubMed

    Mtimet, Narjes; Trunet, Clément; Mathot, Anne-Gabrielle; Venaille, Laurent; Leguérinel, Ivan; Coroller, Louis; Couvert, Olivier

    2016-06-01

    Geobacillus stearothermophilus spores are recognized as one of the most wet-heat resistant among aerobic spore-forming bacteria and are responsible for 35% of canned food spoilage after incubation at 55 °C. The purpose of this study was to investigate and model the fate of heat-treated survivor spores of G. stearothermophilus ATCC 12980 in growth-preventing environment. G. stearothermophilus spores were heat-treated at four different conditions to reach one or two decimal reductions. Heat-treated spores were stored in nutrient broth at different temperatures and pH under growth-preventing conditions. Spore survival during storage was evaluated by count plating over a period of months. Results reveal that G. stearothermophilus spores surviving heat treatment lose their viability during storage under growth-preventing conditions. Two different subpopulations were observed during non-thermal inactivation. They differed according to the level of their resistance to storage stress, and the proportion of each subpopulation can be modulated by heat treatment conditions. Finally, tolerance to storage stress under growth-preventing conditions increases at refrigerated temperature and neutral pH regardless of heat treatment conditions. Such results suggest that spore inactivation due to heat treatment could be completed by storage under growth-preventing conditions. Copyright © 2016 Elsevier Ltd. All rights reserved.

  12. Sorbent Material Property Requirements for On-Board Hydrogen Storage for Automotive Fuel Cell Systems.

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Ahluwalia, R. K.; Peng, J-K; Hua, T. Q.

    2015-05-25

    Material properties required for on-board hydrogen storage in cryogenic sorbents for use with automotive polymer electrolyte membrane (PEM) fuel cell systems are discussed. Models are formulated for physical, thermodynamic and transport properties, and for the dynamics of H-2 refueling and discharge from a sorbent bed. A conceptual storage configuration with in-bed heat exchanger tubes, a Type-3 containment vessel, vacuum insulation and requisite balance-of-plant components is developed to determine the peak excess sorption capacity and differential enthalpy of adsorption for 5.5 wt% system gravimetric capacity and 55% well-to-tank (WTT) efficiency. The analysis also determines the bulk density to which the materialmore » must be compacted for the storage system to reach 40 g.L-1 volumetric capacity. Thermal transport properties and heat transfer enhancement methods are analyzed to estimate the material thermal conductivity needed to achieve 1.5 kg.min(-1) H-2 refueling rate. Operating temperatures and pressures are determined for 55% WTT efficiency and 95% usable H-2. Needs for further improvements in material properties are analyzed that would allow reduction of storage pressure to 50 bar from 100 bar, elevation of storage temperature to 175-200 K from 150 K, and increase of WTT efficiency to 57.5% or higher.« less

  13. Droplet-size distribution and stability of commercial injectable lipid emulsions containing fish oil.

    PubMed

    Gallegos, Críspulo; Valencia, Concepción; Partal, Pedro; Franco, José M; Maglio, Omay; Abrahamsson, Malin; Brito-de la Fuente, Edmundo

    2012-08-01

    The droplet size of commercial fish oil-containing injectable lipid emulsions, including conformance to United States Pharmacopeia (USP) standards on fat-globule size, was investigated. A total of 18 batches of three multichamber parenteral products containing the emulsion SMOFlipid as a component were analyzed. Samples from multiple lots of the products were evaluated to determine compliance with standards on the volume-weighted percentage of fat exceeding 0.05% (PFAT(5)) specified in USP chapter 729 to ensure the physical stability of i.v. lipid emulsions. The products were also analyzed to determine the effects of various storage times (3, 6, 9, and 12 months) and storage temperatures (25, 30, and 40 °C) on product stability. Larger-size lipid particles were quantified via single-particle optical sensing (SPOS). The emulsion's droplet-size distribution was determined via laser light scattering. SPOS and light-scattering analysis demonstrated mean PFAT(5) values well below USP-specified globule-size limits for all the tested products under all study conditions. In addition, emulsion aging at any storage temperature in the range studied did not result in a significant increase of PFAT(5) values, and mean droplet-size values did not change significantly during storage of up to 12 months at temperatures of 25-40 °C. PFAT(5) values were below the USP upper limits in SMOFlipid samples from multiple lots of three multichamber products after up to 12 months of storage at 25 or 30 °C or 6 months of storage at 40 °C.

  14. Changes in the free amino acid contents of honeys during storage at ambient temperature.

    PubMed

    Iglesias, M Teresa; Martín-Alvarez, Pedro J; Polo, M Carmen; de Lorenzo, Cristina; Gonzalez, Montserrat; Pueyo, Encarnación

    2006-11-29

    This study was carried out to establish the changes in the free amino acid contents of floral honeys, honeydew honeys, and blend honeys during storage at room temperature and to test the capacity of the amino acids to distinguish the origin of the honeys after storage. For this purpose, 54 artisanal honeys (39 floral, 5 honeydew, and 10 blend) were studied. Samples were taken from recently collected honeys and at 3, 6, 9, 12, 16, 20, and 24 months after harvesting. The contents of most of the free amino acids were found to decrease with storage time, with the greatest reduction observed in the first 9 months. The contents of the amino acids aspartic acid, beta-alanine, and proline increased in the first few months after storage, reaching maximum values at 6 months, suggesting the possible existence of enzymatic activities. The application of stepwise discriminant analysis to the free amino acid content data demonstrated that the contents of the amino acids valine, beta-alanine, gamma-aminobutyric acid, serine, isoleucine, alpha-alanine, ornithine, and glutamine correctly assigned 87% of honeys to their group of origin: floral, honeydew, or blend.

  15. Fate of Listeria monocytogenes on Fresh Apples under Different Storage Temperatures.

    PubMed

    Sheng, Lina; Edwards, Katheryn; Tsai, Hsieh-Chin; Hanrahan, Ines; Zhu, Mei-Jun

    2017-01-01

    Fresh apples are typically stored for up to 1 year commercially; different apple varieties require different storage temperatures to maintain their quality characteristics. There is sparse information available about Listeria monocytogenes survival on fresh apples under various storage temperatures. The objective of this study was to comprehensively evaluate the effect of storage temperature on apple fruit decay and L. monocytogenes survival. Unwaxed apple fruits of selected varieties (Fuji and Granny Smith) were dip inoculated in a three-strain L. monocytogenes cocktail to establish ∼3.5 and 6.0 Log 10 CFU/apple. Twenty-four hours post-inoculation, apples were subjected to 1, 4, 10, or 22°C storage for up to 3 months. Apples under the different storage treatments were sampled at 1-, 4-, 7- and 14-day for short-term storage under all four tested temperatures, and 2-, 4-, 8-, and 12-week for long-term storage at 1, 4, and 10°C. A set of uninoculated and unwaxed apples were simultaneously subjected to the previously mentioned storage temperatures and sampled biweekly for their total bacterial count (TPC) and yeasts/molds (Y/M) count. During the 2-week short-term storage, L. monocytogenes population on organic Granny Smith apples stored at 1, 4, or 10°C was reduced by 0.2-0.3 Log. When apples were stored at 22°C, there was a 0.5-1.2 Log 10 CFU/apple reduction 14-day post storage dependent on the initial inoculation level. During the 12-week cold storage under 1, 4, and 10°C, L. monocytogenes count on organic Granny Smith apples decreased by 0.5-1.5 Log 10 CFU/apple for both inoculation levels. L. monocytogenes had similar survival pattern on conventional Granny Smith and Fuji apples with 0.8-2.0 Log 10 CFU/apple reduction over a 3-month cold storage period. Interestingly, both TPC and Y/M count were stable regardless of apple variety or cultivation practice during the 12-week storage at all tested temperatures. In summary, while L. monocytogenes did not proliferate on apple surfaces during 12 weeks of refrigerated storage, only a limited reduction of L. monocytogenes was observed in this study. Therefore, the apple industry cannot rely on cold storage alone to control this pathogen. Additional interventions are needed to eradicate Listeria on fresh apples during long-term cold storage.

  16. Heat storage in the Hettangian aquifer in Berlin - results from a column experiment

    NASA Astrophysics Data System (ADS)

    Milkus, Chri(Sch)augott

    2015-04-01

    Aquifer Thermal Energy Storage (ATES) is a sustainable alternative for storage and seasonal availability of thermal energy. However, its impact on the subsurface flow regime is not well known. In Berlin (Germany), the Jurassic (Hettangian) sandstone aquifer with highly mineralized groundwater (TDS 27 g/L) is currently used for heat storage. The aim of this study was to examine the hydrogeochemical changes that are caused by the induced temperature shift and its effects on the hydraulic permeability of the aquifer. Column experiments were conducted, in which stainless steel columns were filled with sediment from the aquifer and flushed with native groundwater for several weeks. The initial temperature of the experiment was 20°C, comparable to the in-situ conditions within the aquifer. After reaching equilibrium between sediment and water, the temperature was increased to simulate heating of the aquifer. During the experiment, physical and chemical parameters (pH, ORP, dissolved oxygen and dissolved carbon dioxide) were measured at the outflow of the column and the effluent water was sampled. Using a Scanning Electron Microscope, the deposition of precipitated minerals and biofilm on sediment grains was analyzed. Changes in hydraulic properties of the sediment were studied by the use of tracer tests with Uranin.

  17. Hydrogen Storage Performance in Pd/Graphene Nanocomposites.

    PubMed

    Zhou, Chunyu; Szpunar, Jerzy A

    2016-10-05

    We have developed a Pd-graphene nanocomposite for hydrogen storage. The spherically shaped Pd nanoparticles of 5-45 nm in size are homogeneously distributed over the graphene matrix. This new hydrogen storage system has favorable features like desirable hydrogen storage capacity, ambient conditions of hydrogen uptake, and low temperature of hydrogen release. At a hydrogen charging pressure of 50 bar, the material could yield a gravimetric density of 6.7 wt % in the 1% Pd/graphene nanocomposite. As we increased the applied pressure to 60 bar, the hydrogen uptake capacity reached 8.67 wt % in the 1% Pd/graphene nanocomposite and 7.16 wt % in the 5% Pd/graphene nanocomposite. This system allows storage of hydrogen in amounts that exceed the capacity of the gravimetric target announced by the U.S. Department of Energy (DOE).

  18. Thermal Analysis of ZPPR High Pu Content Stored Fuel

    DOE PAGES

    Solbrig, Charles W.; Pope, Chad L.; Andrus, Jason P.

    2014-09-17

    The Zero Power Physics Reactor (ZPPR) operated from April 18, 1969, until 1990. ZPPR operated at low power for testing nuclear reactor designs. This paper examines the temperature of Pu content ZPPR fuel while it is in storage. Heat is generated in the fuel due to Pu and Am decay and is a concern for possible cladding damage. Damage to the cladding could lead to fuel hydriding and oxidizing. A series of computer simulations were made to determine the range of temperatures potentially occuring in the ZPPR fuel. The maximum calculated fuel temperature is 292°C (558°F). Conservative assumptions in themore » model intentionally overestimate temperatures. The stored fuel temperatures are dependent on the distribution of fuel in the surrounding storage compartments, the heat generation rate of the fuel, and the orientation of fuel. Direct fuel temperatures could not be measured but storage bin doors, storage sleeve doors, and storage canister temperatures were measured. Comparison of these three temperatures to the calculations indicates that the temperatures calculated with conservative assumptions are, as expected, higher than the actual temperatures. The maximum calculated fuel temperature with the most conservative assumptions is significantly below the fuel failure criterion of 600°C (1,112°F).« less

  19. Storage temperature and 1-MCP treatment affect storage disorders and physiological attributes of ‘Royal Gala’ apples

    USDA-ARS?s Scientific Manuscript database

    ‘Royal Gala’ apples [Malus domestica (Borkh.) Mansf.] can develop postharvest disorders such as flesh browning, senescent breakdown, peeling, cracking, or shriveling during and after cold storage. The objective of this study was to examine the effects of storage temperature and a range of 1-methylc...

  20. Uncertainty quantification methodologies development for stress corrosion cracking of canister welds

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Dingreville, Remi Philippe Michel; Bryan, Charles R.

    2016-09-30

    This letter report presents a probabilistic performance assessment model to evaluate the probability of canister failure (through-wall penetration) by SCC. The model first assesses whether environmental conditions for SCC – the presence of an aqueous film – are present at canister weld locations (where tensile stresses are likely to occur) on the canister surface. Geometry-specific storage system thermal models and weather data sets representative of U.S. spent nuclear fuel (SNF) storage sites are implemented to evaluate location-specific canister surface temperature and relative humidity (RH). As the canister cools and aqueous conditions become possible, the occurrence of corrosion is evaluated. Corrosionmore » is modeled as a two-step process: first, pitting is initiated, and the extent and depth of pitting is a function of the chloride surface load and the environmental conditions (temperature and RH). Second, as corrosion penetration increases, the pit eventually transitions to a SCC crack, with crack initiation becoming more likely with increasing pit depth. Once pits convert to cracks, a crack growth model is implemented. The SCC growth model includes rate dependencies on both temperature and crack tip stress intensity factor, and crack growth only occurs in time steps when aqueous conditions are predicted. The model suggests that SCC is likely to occur over potential SNF interim storage intervals; however, this result is based on many modeling assumptions. Sensitivity analyses provide information on the model assumptions and parameter values that have the greatest impact on predicted storage canister performance, and provide guidance for further research to reduce uncertainties.« less

  1. Whole-body pre-cooling and heat storage during self-paced cycling performance in warm humid conditions.

    PubMed

    Kay, D; Taaffe, D R; Marino, F E

    1999-12-01

    The aim of this study was to establish the effect that pre-cooling the skin without a concomitant reduction in core temperature has on subsequent self-paced cycling performance under warm humid (31 degrees C and 60% relative humidity) conditions. Seven moderately trained males performed a 30 min self-paced cycling trial on two separate occasions. The conditions were counterbalanced as control or whole-body pre-cooling by water immersion so that resting skin temperature was reduced by approximately 5-6 degrees C. After pre-cooling, mean skin temperature was lower throughout exercise and rectal temperature was lower (P < 0.05) between 15 and 25 min of exercise. Consequently, heat storage increased (P < 0.003) from 84.0+/-8.8 W x m(-2) to 153+/-13.1 W x m(-2) (mean +/- s(mean)) after pre-cooling, while total body sweat fell from 1.7+/-0.1 l x h(-1) to 1.2+/-0.1 l h(-1) (P < 0.05). The distance cycled increased from 14.9+/-0.8 to 15.8+/-0.7 km (P < 0.05) after pre-cooling. The results indicate that skin pre-cooling in the absence of a reduced rectal temperature is effective in reducing thermal strain and increasing the distance cycled in 30 min under warm humid conditions.

  2. Estimation of shelf life of wikau maombo brownies cake using Accelerated Shelf Life Testing (ASLT) method with Arrhenius model

    NASA Astrophysics Data System (ADS)

    Wahyuni, S.; Holilah; Asranudin; Noviyanti

    2018-02-01

    The shelf life of brownies cake made from wikau maombo flour was predicted by ASLT method through the Arrhenius model. The aim of this study was to estimate the shelf life of brownies cake made from wikau maombo flour. The storage temperature of brownies cake was carried out at 20°C, 30°C and 45°C. The results showed that TBA (Thio Barbaturic Acid) number of brownies cake decreased as the storage temperature increase. Brownies stored at 20°C and 30°C were overgrown with mold on the storage time of six days. Brownies product (WT0 and WT1) had shelf life at 40°C approximately six and fourteen days, respectively. Brownies made from wikau maombo and wheat flour (WT1) was the best product with had the longest of shelf life about fourteen days.

  3. Improved platelet survival after cold storage by prevention of glycoprotein Ibα clustering in lipid rafts

    PubMed Central

    Gitz, Eelo; Koekman, Cornelis A; van den Heuvel, Dave J.; Deckmyn, Hans; Akkerman, Jan W.; Gerritsen, Hans C.; Urbanus, Rolf T.

    2012-01-01

    Background Storing platelets for transfusion at room temperature increases the risk of microbial infection and decreases platelet functionality, leading to out-date discard rates of up to 20%. Cold storage may be a better alternative, but this treatment leads to rapid platelet clearance after transfusion, initiated by changes in glycoprotein Ibα, the receptor for von Willebrand factor. Design and Methods: We examined the change in glycoprotein Ibα distribution using Förster resonance energy transfer by time-gated fluorescence lifetime imaging microscopy. Results Cold storage induced deglycosylation of glycoprotein Ibα ectodomain, exposing N-acetyl-Dglucosamine residues, which sequestered with GM1 gangliosides in lipid rafts. Raft-associated glycoprotein Ibα formed clusters upon binding of 14-3-3ζ adaptor proteins to its cytoplasmic tail, a process accompanied by mitochondrial injury and phosphatidyl serine exposure. Cold storage left glycoprotein Ibα surface expression unchanged and although glycoprotein V decreased, the fall did not affect glycoprotein Ibα clustering. Prevention of glycoprotein Ibα clustering by blockade of deglycosylation and 14-3-3ζ translocation increased the survival of cold-stored platelets to above the levels of platelets stored at room temperature without compromising hemostatic functions. Conclusions We conclude that glycoprotein Ibα translocates to lipid rafts upon cold-induced deglycosylation and forms clusters by associating with 14-3-3ζ. Interference with these steps provides a means to enable cold storage of platelet concentrates in the near future. PMID:22733027

  4. Storage beyond three hours at ambient temperature alters the biochemical and nutritional qualities of breast milk.

    PubMed

    Eteng, M U; Ebong, P E; Eyong, E U; Ettarh, R R

    2001-08-01

    The effect of storage on stability of human breast milk was investigated in 30 lactating mothers. Samples stored for 3, 6 and 24 hours at ambient temperature of 302K (29 degrees) were analysed for protein, lactose, pH, and microbial content. There were significant (p < 0.01) decreases in protein, lactose and pH upon storage for 6 and 24 hours, compared with storage for 3 hours as control. The mean +/- SEM values for protein for 6 and 24 hours were 15.56 +/- 0.48 and 13.27 +/- 0.50, compared with 17.26 +/- 0.41 for 3 hours. For lactose, corresponding values for 6 and 24 hours were 0.08 +/- 0.005 and 0.07 +/- 0.006, compared with 3 hours (0.09 +/- 0.005). The pH values were 6.1 +/- 0.09, 5.9 +/- 0.07 in 3, 6 and 24 hour samples rspectively. The skin floras investigated were Streptococcus viridians, Straphylococcus aureus and Staphylococcus albus. The microbial content increased with increase in storage time from 3 to 24 hours. The predominant bacterial specie was S. Albus, followed by S.viridians and S. aureus. A positive correlation (r = 0.453, p < 0.01) between lactose level and pH were obtained. These results suggest that breast milk is stable for 3 hours, beyond which significant changes occur in its biochemical composition and nutritional quality. The implications of these findings are discussed with respect to its consequences on their child's survival.

  5. Impact of Spodoptera frugiperda neonate pretreatment conditions on Vip3Aa19 insecticidal protein activity and laboratory bioassay variation.

    PubMed

    Da Silva, Karen F; Spencer, Terence A; Camargo Gil, Carolina; Siegfried, Blair D; Walters, Frederick S

    2016-04-01

    Variation in response to insecticidal proteins is common upon repetition of insect bioassays. Understanding this variation is a prerequisite to detecting biologically important differences. We tracked neonate Spodoptera frugiperda (J.E. Smith) susceptibility to Vip3Aa19 over 17 generations using standardized bioassay methods. Five larval pretreatment conditions and one bioassay condition were tested to determine whether susceptibility was affected. These included: storage time; prefeeding; storage at reduced temperature; storage at reduced humidity; colony introgression of field-collected individuals. Extremes of photoperiod during the bioassay itself were also examined. LC50 values for two strains of S. frugiperda varied 6.6-fold or 8.8-fold over 17 generations. Storage time and humidity had no impact on Vip3Aa19 susceptibility, whereas prefeeding significantly reduced subsequent mortality (by 27%). Storage at reduced temperature increased mortality for one colony (from 45.6 to 73.0%) but not for the other. Introgression of field-collected individuals affected susceptibility at the first generation but not for subsequent generations. A 24 h bioassay photophase significantly reduced susceptibility (by 26%) for both colonies. Certain pretreatment and bioassay conditions were identified that can affect S. frugiperda Vip3Aa19 susceptibility, but innate larval heterogeneity was also present. Our observations should help to increase the consistency of insecticidal protein bioassay results. © 2015 Syngenta Crop Protection, LLC. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

  6. Thermal energy storage

    NASA Technical Reports Server (NTRS)

    Grodzka, P. G.; Picklesimer, E. A.

    1978-01-01

    The general scope of study on thermal energy storage development includes: (1) survey and review possible concepts for storing thermal energy; (2) evaluate the potentials of the surveyed concepts for practical applications in the low and high temperature ranges for thermal control and storage, with particular emphasis on the low temperature range, and designate the most promising concepts; and (3) determine the nature of further studies required to expeditiously convert the most promising concept(s) to practical applications. Cryogenic temperature control by means of energy storage materials was also included.

  7. RECONSTRUCTION AND ANALYSIS OF HISTORICAL CHANGES IN CARBON STORAGE IN ARCTIC TUNDRA

    EPA Science Inventory

    Surface air temperature in arctic regions has increased since pre-industrial times, raising concerns that warmer and possibly drier conditions have increased soil decomposition rates, thereby stimulating the release to the atmosphere of the large stores of carbon (C) in arctic so...

  8. Evidence that exercise-induced heat storage is dependent on adrenomedullary secretion.

    PubMed

    Rodrigues, A G; Lima, N R V; Coimbra, C C; Marubayashi, U

    2008-06-09

    To investigate the influence of medullary adrenal secretion on thermoregulation during exercise, Phy (Eserine, 5x10(-3) M) was injected into the lateral cerebral ventricle of normal (INT) or bilaterally adrenodemedullated (ADM) untrained rats. Body temperature (Tb) and metabolic rate were measured in the rats while they were exercising on a treadmill (20 m min(-1), 5% inclination) until fatigue or while they were at rest after drug injection. In resting rats, Phy increased oxygen consumption in both INT or ADM rats without any effect on core temperature. During the dynamic phase of exercise (first 20 min), ADM attenuated the exercise-induced increase in core temperature (0.86+/-0.12 degrees C ADM Sal vs 1.48+/-0.21 degrees C INT Sal), thus reducing heat storage (HS) levels. Icv injection of Phy in ADM rats significantly reduced the increase in Tb (0.012+/-0.10 degrees C min(-1) Phy vs 0.042+/-0.006 degrees C min(-1) Sal; p<0.02) and HS (65.8+/-56.1 cal Phy vs 207.7+/-32.7 cal Sal; p<0.04) compared to ADM Sal rats. In conclusion, the exercise-induced increase in heat storage was attenuated by adrenodemedullation in rats. Furthermore, the activation of heat loss mechanisms by the central cholinergic system during exercise occurs independently of adrenal medullary secretion suppression and can be improved by previous adrenodemedullation. Our data indicate the existence of a dual mechanism of heat loss control during the dynamic phase of exercise: one involving sympathoadrenal system activation that impairs heat loss and another that counteracts the increased sympathoadrenal activity through the hypothalamic cholinergic system to promote heat loss.

  9. INFLUENCE OF INTRAMUSCULAR FAT LEVEL ON ORGANOLEPTIC, PHYSICAL, AND CHEMICAL CHARACTERISTICS OF IRRADIATED PORK. I. HIGH-TEMPERATURE SHORT-TIME PRE-IRRADIATION HEAT TREATMENT

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Bray, R.W.; Weckel, K.G.; Evans, G.W.

    1964-02-01

    The influence of intramuscular fat (degree of marbling) on characteristics of precooked and irradiated pork muscle was studied. Loins were selected and categorized into three marbling levels by visual appraisal. A relatively high temperature (325 deg F) and short time (2 hr) heat treatment was used for enzyme inactivation. Samples were packed under vacuum in rigid containers and irradiated to 4.5 Mrad with gamma radiation. Irradiated and frozen control samples were evaluated up to 2l0 days later. Degree of marbling had no apparent influence on organoleptic properties of either irradiated or frozen control longissimus dorsi muscle samples. Frozen control samplesmore » were preferred in general appearance, flavor, and over-all acceptability by panelists. Irradiated samples were preferred in texture qualities. Storage time was not a major factor in organoleptic acceptability; however, acceptability of irradiated samples declined between 150 and 210 days of storage. Hunter color attributes were not affected by marbling level. L, a/sub L/ hue, and saturation were increased by radiation treatment. Mechanical tenderness values were decreased due to higher marbling level and radiation treatment. Expressible-moisture values were lowered by radiation treatment and increased with storage time. Iodine numbers were decreased by radiation. Degree of marbling did not affect thiobarbituric acid values but they were significantly lower for irradiated samples. pH values increased with higher levels of intramuscular fat, were significantly higher in irradiated samples than controls, and tended to increase steadily with advancing storage time. (BBB)« less

  10. Effect of storage temperature and time on the nutritional quality of walnut male inflorescences.

    PubMed

    Zhang, Wen-E; Wang, Chang-Lei; Shi, Bin-Bin; Pan, Xue-Jun

    2017-04-01

    The objective of this study was to investigate the effect of storage temperature and time on nutrients, bioactive compounds, and antioxidant activities of walnut male inflorescences. The results showed that the moisture, saccharides, fat, protein, amino acids, ascorbic acid, phenolic and flavonoid compound contents, and antioxidant activities of walnut male inflorescences were markedly influenced by storage temperature, and different degrees of decrease in these parameters were observed during the entire storage period. Moreover, higher storage temperature had a more significant effect on the nutrients, bioactive compounds, and antioxidant activities of walnut male flowers, and the loss rate of these components at 25°C was higher than that determined at 4°C. However, the results also presented that the ash and mineral contents did not appear to be influenced significantly by the storage temperature, and slightly significant changes were observed in crude fiber throughout storage, which indicated that the influence of storage on the individual mineral and crude fiber content was minimal. Based on the findings in this study, in order to maximize nutrients concentration, walnut male inflorescences should be kept at 4°C for <6 days and be consumed as fresh as possible. Copyright © 2016. Published by Elsevier B.V.

  11. Technology Application of Environmental Friendly Refrigeration (Green Refrigeration) on Cold Storage for Fishery Industry

    NASA Astrophysics Data System (ADS)

    Rasta, IM; Susila, IDM; Subagia, IWA

    2018-01-01

    The application of refrigeration technology to postharvest fishery products is an very important. Moreover, Indonesia is a tropical region with relatively high temperatures. Fish storage age can be prolonged with a decrease in temperature. Frozen fish can even be stored for several months. Fish freezing means preparing fish for storage in low-temperature cold storage. The working fluid used in cold storage to cool low-temperature chambers and throw heat into high-temperature environments is refrigerant. So far refrigerant used in cold storage is Hydrochloroflourocarbons (HCFC) that is R-22. Chlor is a gas that causes ODP (Ozone Depleting Potential), while Flour is a gas that causes GWP (Global Warming Potential). Government policy began in 2015 to implement Hydrochloroflourocarbons Phase-Out Management Plan. Hydrocarbon (HC) is an alternative substitute for R-22. HC-22 (propane ≥ 99.5%) has several advantages, among others: environmentally friendly, indicated by a zero ODP value, and GWP = 3 (negligible), thermophysical property and good heat transfer characteristics, vapor phase density Which is low, and good solubility with mineral lubricants. The use of HC-22 in cold storage is less than R-22. From the analysis results obtained, cold storage system using HC-22 has better performance and energy consumption is more efficient than the R-22.

  12. Active compound diffusivity of particle size reduced S. aromaticum and C. cassia fused starch edible films and the shelf life of mutton (Capra aegagrus hircus) meat.

    PubMed

    Chandra Mohan, C; Radha Krishnan, K; Babuskin, S; Sudharsan, K; Aafrin, Vajiha; Lalitha Priya, U; Mariyajenita, P; Harini, K; Madhushalini, D; Sukumar, M

    2017-06-01

    In the present study, mathematical models were used to examine the effect of active compound diffusion from edible film (supplemented with S. aromaticum and C. cassia) on the microbial, physical and chemical quality of mutton stored at 4 and 10°C. Cinnamaldehyde and eugenol release from edible film into liquid was found to be 80% and 75% of the equilibrium concentration. Active compound release into meat was 42-51% for cinnamaldehyde and 38-48% for eugenol, in storage temperatures of 4-15°C. Developed mathematical models showed the diffusivity of cinnamaldehyde (0.45×10 -15 ±0.04×10 -15 ) and eugenol (0.63×10 -10 ±0.01×10 -10 ) into meat, which was about 40% of that in liquid medium. On comparing physical, chemical and microbial results, shelf life of mutton meat was found to be increased by 1week at storage temperature of 10°C and 3weeks at storage temperature of 4°C. Copyright © 2017 Elsevier Ltd. All rights reserved.

  13. High temperature latent heat thermal energy storage to augment solar thermal propulsion for microsatellites

    NASA Astrophysics Data System (ADS)

    Gilpin, Matthew R.

    Solar thermal propulsion (STP) offers an unique combination of thrust and efficiency, providing greater total DeltaV capability than chemical propulsion systems without the order of magnitude increase in total mission duration associated with electric propulsion. Despite an over 50 year development history, no STP spacecraft has flown to-date as both perceived and actual complexity have overshadowed the potential performance benefit in relation to conventional technologies. The trend in solar thermal research over the past two decades has been towards simplification and miniaturization to overcome this complexity barrier in an effort finally mount an in-flight test. A review of micro-propulsion technologies recently conducted by the Air Force Research Laboratory (AFRL) has identified solar thermal propulsion as a promising configuration for microsatellite missions requiring a substantial Delta V and recommended further study. A STP system provides performance which cannot be matched by conventional propulsion technologies in the context of the proposed microsatellite ''inspector" requiring rapid delivery of greater than 1500 m/s DeltaV. With this mission profile as the target, the development of an effective STP architecture goes beyond incremental improvements and enables a new class of microsatellite missions. Here, it is proposed that a bi-modal solar thermal propulsion system on a microsatellite platform can provide a greater than 50% increase in Delta V vs. chemical systems while maintaining delivery times measured in days. The realization of a microsatellite scale bi-modal STP system requires the integration of multiple new technologies, and with the exception of high performance thermal energy storage, the long history of STP development has provided "ready" solutions. For the target bi-modal STP microsatellite, sensible heat thermal energy storage is insufficient and the development of high temperature latent heat thermal energy storage is an enabling technology for the platform. The use of silicon and boron as high temperature latent heat thermal energy storage materials has been in the background of solar thermal research for decades without a substantial investigation. This is despite a broad agreement in the literature about the performance benefits obtainable from a latent heat mechanisms which provides a high energy storage density and quasi-isothermal heat release at high temperature. In this work, an experimental approach was taken to uncover the practical concerns associated specifically with applying silicon as an energy storage material. A new solar furnace was built and characterized enabling the creation of molten silicon in the laboratory. These tests have demonstrated the basic feasibility of a molten silicon based thermal energy storage system and have highlighted asymmetric heat transfer as well as silicon expansion damage to be the primary engineering concerns for the technology. For cylindrical geometries, it has been shown that reduced fill factors can prevent damage to graphite walled silicon containers at the expense of decreased energy storage density. Concurrent with experimental testing, a cooling model was written using the "enthalpy method" to calculate the phase change process and predict test section performance. Despite a simplistic phase change model, and experimentally demonstrated complexities of the freezing process, results coincided with experimental data. It is thus possible to capture essential system behaviors of a latent heat thermal energy storage system even with low fidelity freezing kinetics modeling allowing the use of standard tools to obtain reasonable results. Finally, a technological road map is provided listing extant technological concerns and potential solutions. Improvements in container design and an increased understanding of convective coupling efficiency will ultimately enable both high temperature latent heat thermal energy storage and a new class of high performance bi-modal solar thermal spacecraft.

  14. Atomistic simulations of aromatic polyurea and polyamide for capacitive energy storage

    NASA Astrophysics Data System (ADS)

    Dong, Rui; Ranjan, V.; Buongiorno Nardelli, Marco; Bernholc, J.

    2015-07-01

    Materials for capacitive energy storage with high energy density and low loss are desired in many fields. We investigate several polymers with urea and amide functional groups using density functional theory and classical molecular dynamics simulations. For aromatic polyurea (APU) and para-aramid (PA), we find several nearly energetically degenerate ordered structures, while meta-aromatic polyurea (mAPU) tends to be rotationally disordered along the polymer chains. Simulated annealing of APU and PA structures results in the formation of hydrogen-bonded sheets, highlighting the importance of dipole-dipole interactions. In contrast, hydrogen bonding does not play a significant role in mAPU, hence the propensity to disorder. We find that the disordered structures with misaligned chains have significantly larger dielectric constants, due to significant increase in the free volume, which leads to easier reorientation of dipolar groups in the presence of an electric field. Large segment motion is still not allowed below the glass transition temperature, which explains the experimentally observed very low loss at high field and elevated temperature. However, the degree of disorder needs to be controlled, because highly entangled structures diminish the free dipoles and decrease permittivity. Among the considered materials, mAPU is the most promising dielectric for capacitive energy storage, but the concept of increasing permittivity while maintaining low loss through disorder-induced free volume increase is generally applicable and provides an alternative pathway for the design of high-performance dielectrics for capacitive energy storage.

  15. Thermal energy storage. [by means of chemical reactions

    NASA Technical Reports Server (NTRS)

    Grodzka, P. G.

    1975-01-01

    The principles involved in thermal energy storage by sensible heat, chemical potential energy, and latent heat of fusion are examined for the purpose of evolving selection criteria for material candidates in the low ( 0 C) and high ( 100 C) temperature ranges. The examination identifies some unresolved theoretical considerations and permits a preliminary formulation of an energy storage theory. A number of candidates in the low and high temperature ranges are presented along with a rating of candidates or potential candidates. A few interesting candidates in the 0 to 100 C region are also included. It is concluded that storage by means of reactions whose reversibility can be controlled either by product removal or by catalytic means appear to offer appreciable advantages over storage with reactions whose reversability cannot be controlled. Among such advantages are listed higher heat storage capacities and more favorable options regarding temperatures of collection, storage, and delivery. Among the disadvantages are lower storage efficiencies.

  16. Thermal storage requirements for parabolic dish solar power plants

    NASA Technical Reports Server (NTRS)

    Wen, L.; Steele, H.

    1980-01-01

    The cost effectiveness of a high temperature thermal storage system is investigated for a representative parabolic dish solar power plant. The plant supplies electrical power in accordance with a specific, seasonally varying demand profile. The solar power received by the plant is supplemented by power from fuel combustion. The cost of electricity generated by the solar power plant is calculated, using the cost of mass-producible subsystems (specifically, parabolic dishes, receivers, and power conversion units) now being designed for this type of solar plant. The trade-off between fuel and thermal storage is derived in terms of storage effectiveness, the cost of storage devices, and the cost of fuel. Thermal storage requirements, such as storage capacity, storage effectiveness, and storage cost are established based on the cost of fuel and the overall objective of minimizing the cost of the electricity produced by the system. As the cost of fuel increases at a rate faster than general inflation, thermal storage systems in the $40 to $70/kWthr range could become cost effective in the near future.

  17. Retrofitting a Geothermal Plant with Solar and Storage to Increase Power Generation

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Zhu, Guangdong; McTigue, Joshua Dominic P; Turchi, Craig S

    Solar hybridization using concentrating solar power (CSP) can be an effective approach to augment the power generation and power cycle efficiency of a geothermal power plant with a declining resource. Thermal storage can further increase the dispatchability of a geothermal/solar hybrid system, which is particularly valued for a national grid with high renewable penetration. In this paper, a hybrid plant design with thermal storage is proposed based on the requirements of the Coso geothermal field in China Lake, California. The objective is to increase the power production by 4 MWe. In this system, a portion of the injection brine ismore » recirculated through a heat exchanger with the solar heat transfer fluid, before being mixed with the production well brine. In the solar heating loop the brine should be heated to at least 155 degrees C to increase the net power. The solar field and storage were sized based on solar data for China Lake. Thermal storage is used to store excess power at the high-solar-irradiation hours and generate additional power during the evenings. The solar field size, the type and capacity of thermal storage and the operating temperatures are critical factors in determining the most economic hybrid system. Further investigations are required to optimize the hybrid system and evaluate its economic feasibility.« less

  18. Aboveground carbon sequestration in dry temperate forests varies with climate not fire regime.

    PubMed

    Gordon, Christopher E; Bendall, Eli R; Stares, Mitchell G; Collins, Luke; Bradstock, Ross A

    2018-06-01

    The storage of carbon in plant tissues and debris has been proposed as a method to offset anthropogenic increases in atmospheric [CO 2 ]. Temperate forests represent significant above-ground carbon (AGC) "sinks" because their relatively fast growth and slow decay rates optimise carbon assimilation. Fire is a common disturbance event in temperate forests globally that should strongly influence AGC because: discrete fires consume above-ground biomass releasing carbon to the atmosphere, and the long-term application of different fire-regimes select for specific plant communities that sequester carbon at different rates. We investigated the latter process by quantifying AGC storage at 104 sites in the Sydney Basin Bioregion, Australia, relative to differences in components of the fire regime: frequency, severity and interfire interval. To predict the potential impacts of future climate change on fire/AGC interactions, we stratified our field sites across gradients of mean annual temperature and precipitation and quantified within- and between-factor interactions between the fire and climate variables. In agreement with previous studies, large trees were the primary AGC sink, accounting for ~70% of carbon at sites. Generalised additive models showed that mean annual temperature was the strongest predictor of AGC storage, with a 54% near-linear decrease predicted across the 6.1°C temperature range experienced at sites. Mean annual precipitation, fire frequency, fire severity and interfire interval were consistently poor predictors of total above-ground storage, although there were some significant relationships with component stocks. Our results show resilience of AGC to frequent and severe wildfire and suggest temperature mediated decreases in forest carbon storage under future climate change predictions. © 2018 John Wiley & Sons Ltd.

  19. Change in the color of heat-treated, vacuum-packed broccoli stems and florets during storage: effects of process conditions and modeling by an artificial neural network.

    PubMed

    Pero, Milad; Askari, Gholamreza; Skåra, Torstein; Skipnes, Dagbjørn; Kiani, Hossein

    2018-02-08

    Vacuum-packed broccoli stems and florets were subjected to heat treatment (60-99 °C) for various time intervals. The activity of peroxidase was measured after processing. Thermally processed samples were then stored at 4 °C for 35 days, and the color of the samples was measured every 7 days. Effects of parameters (heating temperature and duration, storage time) on the color of broccoli were modeled and simulated by an artificial neural network (ANN). Simulations confirmed that stems were predicted to be more prone to changes than florets. More color loss was observed with longer processing or storage combinations. The simulations also confirmed that higher temperatures during heat processing could retard color changes during storage. For stems treated at 80 °C for short durations, color loss was more predominant than both 65 and 99 °C, probably due to the incomplete inactivation of enzymes besides more tissue damage, with increased enzyme access to the substrate. The greenness of both stems and florets during storage can be better preserved at higher temperatures (99 °C) and short times. The simulation results revealed that the ANN method could be used as an effective tool for predicting and analyzing the color values of heat-treated broccoli. © 2018 Society of Chemical Industry. © 2018 Society of Chemical Industry.

  20. Alginate-Encapsulation for the Improved Hypothermic Preservation of Human Adipose-Derived Stem Cells.

    PubMed

    Swioklo, Stephen; Constantinescu, Andrei; Connon, Che J

    2016-03-01

    Despite considerable progress within the cell therapy industry, unmet bioprocessing and logistical challenges associated with the storage and distribution of cells between sites of manufacture and the clinic exist. We examined whether hypothermic (4°C-23°C) preservation of human adipose-derived stem cells could be improved through their encapsulation in 1.2% calcium alginate. Alginate encapsulation improved the recovery of viable cells after 72 hours of storage. Viable cell recovery was highly temperature-dependent, with an optimum temperature of 15°C. At this temperature, alginate encapsulation preserved the ability for recovered cells to attach to tissue culture plastic on rewarming, further increasing its effect on total cell recovery. On attachment, the cells were phenotypically normal, displayed normal growth kinetics, and maintained their capacity for trilineage differentiation. The number of cells encapsulated (up to 2 × 10(6) cells per milliliter) did not affect viable cell recovery nor did storage of encapsulated cells in a xeno-free, serum-free,current Good Manufacturing Practice-grade medium. We present a simple, low-cost system capable of enhancing the preservation of human adipose-derived stem cells stored at hypothermic temperatures, while maintaining their normal function. The storage of cells in this manner has great potential for extending the time windows for quality assurance and efficacy testing, distribution between the sites of manufacture and the clinic, and reducing the wastage associated with the limited shelf life of cells stored in their liquid state. ©AlphaMed Press.

  1. Storage Duration of Autologous Stem Cell Preparations Has No Impact on Hematopoietic Recovery after Transplantation.

    PubMed

    Lisenko, Katharina; Pavel, Petra; Kriegsmann, Mark; Bruckner, Thomas; Hillengass, Jens; Goldschmidt, Hartmut; Witzens-Harig, Mathias; Ho, Anthony D; Wuchter, Patrick

    2017-04-01

    Peripheral blood stem cells (PBSCs) are widely used for autologous blood stem cell transplantation (ABSCT). These cells must be stored for months or even years, usually at temperatures ≤-140°C, until their use. Although several in vitro studies on CD34 + viability and clonogenic assays of PBSCs after long-term storage have been reported, only a few publications have investigated the influence of long-term storage on in vivo hematopoietic reconstitution. In this study, we retrospectively analyzed hematopoietic recovery after storage of PBSCs via controlled-rate freezing (CRF) and cryostorage in 10% DMSO at ≤-140°C in 105 patients with multiple myeloma who received high-dose melphalan before ABSCT. Three groups of PBSC transplantation (n = 247) were delineated based on the storage period: short-term (≤12 months, n = 143), medium-term (>12 and ≤60 months, n = 75), and long-term storage (>60 months, n = 29). A neutrophil increase of ≥.5 × 10 9 /L in medium-term or long-term PBSC cryopreservation groups was observed at day 14 after ABSCT; this increase was comparable to patients who received briefly stored PBSCs (day 15). No negative effect of PBSC storage duration was observed on leucocyte or neutrophil reconstitution. Platelet reconstitutions of ≥20  × 10 9 /L and 50 × 10 9 /L were observed after median times of 10 to 11 and 13 to 14 days after ABSCT, respectively. No influence of PBSC storage duration on platelet recovery of ≥20  × 10 9 /L and ≥50 × 10 9 /L was observed in the 3 storage groups (P = .07, P = .32). The number of previous ABSCTs also had no significant impact upon hematopoietic reconstitution. In conclusion, these results indicate that long-term cryopreservation of PBSC products at vapor nitrogen temperature after CRF does not have a negative effect on hematopoietic recovery even after prolonged storage. Copyright © 2017 The American Society for Blood and Marrow Transplantation. Published by Elsevier Inc. All rights reserved.

  2. Thermophysical Characterization of MgCl2·6H2O, Xylitol and Erythritol as Phase Change Materials (PCM) for Latent Heat Thermal Energy Storage (LHTES)

    PubMed Central

    Höhlein, Stephan; König-Haagen, Andreas; Brüggemann, Dieter

    2017-01-01

    The application range of existing real scale mobile thermal storage units with phase change materials (PCM) is restricted by the low phase change temperature of 58 ∘C for sodium acetate trihydrate, which is a commonly used storage material. Therefore, only low temperature heat sinks like swimming pools or greenhouses can be supplied. With increasing phase change temperatures, more applications like domestic heating or industrial process heat could be operated. The aim of this study is to find alternative PCM with phase change temperatures between 90 and 150 ∘C. Temperature dependent thermophysical properties like phase change temperatures and enthalpies, densities and thermal diffusivities are measured for the technical grade purity materials xylitol (C5H12O5), erythritol (C4H10O4) and magnesiumchloride hexahydrate (MCHH, MgCl2·6H2O). The sugar alcohols xylitol and erythritol indicate a large supercooling and different melting regimes. The salt hydrate MgCl2·6H2O seems to be a suitable candidate for practical applications. It has a melting temperature of 115.1 ± 0.1 ∘C and a phase change enthalpy of 166.9 ± 1.2 J/g with only 2.8 K supercooling at sample sizes of 100 g. The PCM is stable over 500 repeated melting and solidification cycles at differential scanning calorimeter (DSC) scale with only small changes of the melting enthalpy and temperature. PMID:28772806

  3. The effect of storage temperature on the biological activity of extracellular vesicles for the complement system.

    PubMed

    Park, Sang June; Jeon, Hyungtaek; Yoo, Seung-Min; Lee, Myung-Shin

    2018-05-10

    Extracellular vesicles (EVs) are mediators of intercellular communication by transporting cargo containing proteins, lipids, mRNA, and miRNA. There is increasing evidence that EVs have various roles in regulating migration, invasion, stemness, survival, and immune functions. Previously, we have found that EVs from Kaposi's sarcoma-associated herpesvirus (KSHV)-infected human endothelial cells have the potential to activate the complement system. Although many studies have shown that the physical properties of EVs can be changed by their storage condition, there have been few studies for the stability of biological activity of EVs in various storage conditions. In this study, we investigated various conditions to identify the best conditions to store EVs with functional stability for 25 d. Furthermore, the correlation between the function and other characteristics of EVs, including the expression of EV markers, size distribution, and particle number, were also analyzed. Our results demonstrated that storage temperature is an important factor to maintain the activity of EVs and would be useful information for basic research and clinical application using EVs.

  4. Quality Parameters of Six Cultivars of Blueberry Using Computer Vision

    PubMed Central

    Celis Cofré, Daniela; Silva, Patricia; Enrione, Javier; Osorio, Fernando

    2013-01-01

    Background. Blueberries are considered an important source of health benefits. This work studied six blueberry cultivars: “Duke,” “Brigitta”, “Elliott”, “Centurion”, “Star,” and “Jewel”, measuring quality parameters such as °Brix, pH, moisture content using standard techniques and shape, color, and fungal presence obtained by computer vision. The storage conditions were time (0–21 days), temperature (4 and 15°C), and relative humidity (75 and 90%). Results. Significant differences (P < 0.05) were detected between fresh cultivars in pH, °Brix, shape, and color. However, the main parameters which changed depending on storage conditions, increasing at higher temperature, were color (from blue to red) and fungal presence (from 0 to 15%), both detected using computer vision, which is important to determine a shelf life of 14 days for all cultivars. Similar behavior during storage was obtained for all cultivars. Conclusion. Computer vision proved to be a reliable and simple method to objectively determine blueberry decay during storage that can be used as an alternative approach to currently used subjective measurements. PMID:26904598

  5. Use of Sonoelastography to Evaluate Texture Modifications of Mozzarella di Bufala Campana Protected Designation of Origin During Storage at Different Temperatures

    PubMed Central

    Santoro, Adriano Michele Luigi; Sarno, Eleonora; Di Loria, Antonio; Grembiale, Rosa Daniela; Britti, Domenico; Capuano, Federico

    2014-01-01

    Mozzarella cheese from buffalo milk is a fresh, stringy-textured dairy product, exhibiting a porcelain white colour, a smooth, bright, and humid surface, an extremely thin rind and delicate taste. The high humidity typical of this cheese reduces its shelf-life and it is cause of dramatic organoleptic changes during storage. In this study we tested sonoelastography to evaluate texture changes of mozzarella cheese from buffalo milk during storage. Cheeses form local market produced in the same condition were divided in three batches and stored in different conditions: the first (B1) was stored in preserving liquid at room temperature (20°C); the second (B2) was stored without preserving liquid at 4°C; and the third (B3) was stored at 4°C in preserving liquid. In B1 sonoelastography showed a reduction of the hardness and stiffness of rind, while in B2 inelastic tissue increased its thickness. Best results were obtained in B3, where no significant difference was evidenced during storage. PMID:27800416

  6. Reliable data storage system design and implementation for acoustic logging while drilling

    NASA Astrophysics Data System (ADS)

    Hao, Xiaolong; Ju, Xiaodong; Wu, Xiling; Lu, Junqiang; Men, Baiyong; Yao, Yongchao; Liu, Dong

    2016-12-01

    Owing to the limitations of real-time transmission, reliable downhole data storage and fast ground reading have become key technologies in developing tools for acoustic logging while drilling (LWD). In order to improve the reliability of the downhole storage system in conditions of high temperature, intensive shake and periodic power supply, improvements were made in terms of hardware and software. In hardware, we integrated the storage system and data acquisition control module into one circuit board, to reduce the complexity of the storage process, by adopting the controller combination of digital signal processor and field programmable gate array. In software, we developed a systematic management strategy for reliable storage. Multiple-backup independent storage was employed to increase the data redundancy. A traditional error checking and correction (ECC) algorithm was improved and we embedded the calculated ECC code into all management data and waveform data. A real-time storage algorithm for arbitrary length data was designed to actively preserve the storage scene and ensure the independence of the stored data. The recovery procedure of management data was optimized to realize reliable self-recovery. A new bad block management idea of static block replacement and dynamic page mark was proposed to make the period of data acquisition and storage more balanced. In addition, we developed a portable ground data reading module based on a new reliable high speed bus to Ethernet interface to achieve fast reading of the logging data. Experiments have shown that this system can work stably below 155 °C with a periodic power supply. The effective ground data reading rate reaches 1.375 Mbps with 99.7% one-time success rate at room temperature. This work has high practical application significance in improving the reliability and field efficiency of acoustic LWD tools.

  7. An allowable cladding peak temperature for spent nuclear fuels in interim dry storage

    NASA Astrophysics Data System (ADS)

    Cha, Hyun-Jin; Jang, Ki-Nam; Kim, Kyu-Tae

    2018-01-01

    Allowable cladding peak temperatures for spent fuel cladding integrity in interim dry storage were investigated, considering hydride reorientation and mechanical property degradation behaviors of unirradiated and neutron irradiated Zr-Nb cladding tubes. Cladding tube specimens were heated up to various temperatures and then cooled down under tensile hoop stresses. Cool-down specimens indicate that higher heat-up temperature and larger tensile hoop stress generated larger radial hydride precipitation and smaller tensile strength and plastic hoop strain. Unirradiated specimens generated relatively larger radial hydride precipitation and plastic strain than did neutron irradiated specimens. Assuming a minimum plastic strain requirement of 5% for cladding integrity maintenance in interim dry storage, it is proposed that a cladding peak temperature during the interim dry storage is to keep below 250 °C if cladding tubes are cooled down to room temperature.

  8. Warehouse multipoint temperature and humidity monitoring system design based on Kingview

    NASA Astrophysics Data System (ADS)

    Ou, Yanghui; Wang, Xifu; Liu, Jingyun

    2017-04-01

    Storage is the key link of modern logistics. Warehouse environment monitoring is an important part of storage safety management. To meet the storage requirements of different materials, guarantee their quality in the greatest extent, which has great significance. In the warehouse environment monitoring, the most important parameters are air temperature and relative humidity. In this paper, a design of warehouse multipoint temperature and humidity monitoring system based on King view, which realizes the multipoint temperature and humidity data real-time acquisition, monitoring and storage in warehouse by using temperature and humidity sensor. Also, this paper will take the bulk grain warehouse as an example and based on the data collected in real-time monitoring, giving the corresponding expert advice that combined with the corresponding algorithm, providing theoretical guidance to control the temperature and humidity in grain warehouse.

  9. Synchronized smoldering combustion

    NASA Astrophysics Data System (ADS)

    Mikalsen, R. F.; Hagen, B. C.; Frette, V.

    2018-03-01

    Synchronized, pulsating temperatures are observed experimentally in smoldering fires. The entire sample volume (1.8 l) participates in the pulsations (pulse period 2–4 h). The synchrony lasts up to 25 h and is followed by a spontaneous transition to either disordered combustion or self-extinguishment. The synchronization is obtained when the fuel bed is cooled to the brink of extinguishment. Calculations for adiabatic conditions, including heat generation from combustion (nonlinear in temperature) and heat storage in sample (linear in temperature), predict diverging sample temperature. Experimentally, heat losses to surroundings (linear in temperature) prevent temperatures to increase without bounds and lead to pulsations.

  10. Microbiological quality of raw milk attributable to prolonged refrigeration conditions.

    PubMed

    Vithanage, Nuwan R; Dissanayake, Muditha; Bolge, Greg; Palombo, Enzo A; Yeager, Thomas R; Datta, Nivedita

    2017-02-01

    Refrigerated storage of raw milk is a prerequisite in dairy industry. However, temperature abused conditions in the farming and processing environments can significantly affect the microbiological quality of raw milk. Thus, the present study investigated the effect of different refrigeration conditions such as 2, 4, 6, 8, 10 and 12 °C on microbiological quality of raw milk from three different dairy farms with significantly different initial microbial counts. The bacterial counts (BC), protease activity (PA), proteolysis (PL) and microbial diversity in raw milk were determined during storage. The effect of combined heating (75 ± 0·5 °C for 15 s) and refrigeration on controlling those contaminating microorganisms was also investigated. Results of the present study indicated that all of the samples showed increasing BC, PA and PL as a function of temperature, time and initial BC with a significant increase in those criteria ≥6 °C. Similar trends in BC, PA and PL were observed during the extended storage of raw milk at 4 °C. Both PA and PL showed strong correlation with the psychrotrophic proteolytic count (PPrBC: at ≥4 °C) and thermoduric psychrotrophic count (TDPC: at ≥8 °C) compared to total plate count (TPC) and psychrotrophic bacterial count (PBC), that are often used as the industry standard. Significant increases in PA and PL were observed when PPrBC and TDPC reached 5 × 104 cfu/ml and 1 × 104 cfu/ml, and were defined as storage life for quality (S LQ), and storage life for safety (S LS) aspects, respectively. The storage conditions also significantly affected the microbial diversity, where Pseudomonas fluorescens and Bacillus cereus were found to be the most predominant isolates. However, deep cooling (2 °C) and combination of heating and refrigeration (≤4 °C) significantly extended the S LQ and S Ls of raw milk.

  11. Effects of sample drying and storage, and choice of extraction solvent and analysis method on the yield of birch leaf hydrolyzable tannins.

    PubMed

    Salminen, Juha-Pekka

    2003-06-01

    In this study, I investigated the effects of different methods of sample drying and storage, and the choice of extraction solvent and analysis method on the concentrations of 14 individual hydrolyzable tannins (HTs), and insoluble ellagitannins in birch (Betula pubescens) leaves. Freeze- and vacuum-drying of birch leaves were found to provide more reliable results than air- or oven-drying. Storage of leaves at -20 degrees C for 3 months before freeze-drying did not cause major changes in tannin content, although levels of 1,2,3,4,6-penta-O-galloylglucose and isostrictinin were altered. Storage of dried leaf material at -20 degrees C is preferred because 1 year storage of freeze-dried leaves at 4 degrees C and at room temperature decreased the concentration of the pedunculagin derivative, one of the main ellagitannins of birch. Furthermore, storage at room temperature increased the levels of isostrictinin and 2,3-(S)-HHDP-glucose, indicating possible HT catabolism. Of the extraction solvents tested, aqueous acetone was superior to pure acetone, or aqueous or pure methanol. The addition of 0.1% ascorbic acid into 70% acetone significantly increased the yield of ellagitannins. presumably by preventing their oxidation. By comparing the conventional rhodanine assay and the HPLC-ESI-MS assay for quantification of leaf galloylglucoses, the former tends to underestimate total concentrations of galloylglucoses in birch leaf extract. On the basis of the outcomes of all the method and solvent comparisons, their suitability for qualitative and quantitative analysis of plant HTs is discussed, emphasizing that each plant species, with its presumably unique HT composition, is likely to have a unique combination of ideal conditions for tissue preservation and extraction.

  12. The Roles of Shallow and Deep Groundwater Storage During Drought at Panola Mountain Research Watershed, Georgia, U.S.A.

    NASA Astrophysics Data System (ADS)

    Aulenbach, B. T.; Peters, N. E.

    2016-12-01

    Southeastern U.S. experiences recurring droughts, which can reduce water availability and can result in water-limiting conditions. Monthly water budgets were estimated at Panola Mountain Research Watershed, a small 41-hectare forested watershed near Atlanta, Georgia, from 1985 through 2015, to quantify the effects of climatic variability on groundwater (GW) storage. A relation between stream base flow and watershed GW storage was developed. The relation indicated that both shallow and deep GW storage contribute to base-flow runoff, except for the bottom third (78 mm) of the range in observed shallow soil moisture. The base flow-storage relation was then used to estimate monthly evapotranspiration (ET) using a closed water budget approach. Growing season droughts were almost always preceded by low GW storage at the onset of the growing season. The low base flow and GW storage conditions were caused by low precipitation (P) during the dormant season, and to a lesser extent, carryover of low GW storage conditions from the previous growing season. Growing season P had little impact on drought, as most P ultimately resulted in ET instead of deeper GW recharge. Water-limited growing season conditions were indicated when potential ET (PET) >> ET, and occurred during months having a large "P-deficit", PET - P, and when shallow storage was already near its observed minimum—such that the P-deficits exceeded the extractable water in shallow storage. These observations can be used to hypothesize how projected future increases in temperature, and how resulting increases in PET affect water budgets in Southeastern U.S. The dormant season will become shorter and ET will increase, causing decreased GW recharge during the dormant season, and will result in more frequent and severe growing season droughts. Higher growing season PET would increase the frequency and duration of water limiting conditions due to higher P-deficits and more frequent occurrences of low shallow storage.

  13. An Investigation into the Effects of Temperature and Storage Time on Military Packaged Water in Afghanistan - The Liberation and Migration of Potential Contaminants from Expeditionary Water Packaging System Polyethylene Terephthalate Water Bottles

    DTIC Science & Technology

    2012-03-29

    PET   bottle   degradation   due   to   storage   at   elevated   temperatures.   Results   have   direct... bottle   degradation   of   chemical   and   aesthetic   drinking   water   characteristics   with   extended   storage  at...elevated  temperatures.  To  elucidate  the  impact  of   bottled  water  storage  practices

  14. Low temperature solution synthesis of zinc antimonide, manganese antimonide, and strontium ruthenate compounds

    NASA Astrophysics Data System (ADS)

    Noblitt, Jennifer Lenkner

    2011-12-01

    Increasing energy demands are fueling research in the area of renewable energy and energy storage. In particular, Li-ion batteries and superconducting wires are attractive choices for energy storage. Improving safety, simplifying manufacturing processes, and advancing technology to increase energy storage capacity is necessary to compete with current marketed energy storage devices. These advancements are accomplished through the study of new materials and new morphologies. Increasing dependence on and rising demand for portable electronic devices has continued to drive research in the area of Li-ion batteries. In order to compete with existing batteries and be applicable to future energy needs such as powering hybrid vehicles, the drawbacks of Li-ion batteries must be addressed including (i) low power density, (ii) safety, and (iii) high manufacturing costs. These drawbacks can be addressed through new materials and morphologies for the anode, cathode, and electrolyte. New intermetallic anode materials such as ZnSb, MnSb, and Mn2Sb are attractive candidates to replace graphite, the current industry standard anode material, because they are safer while maintaining comparable theoretical capacity. Electrodeposition is an inexpensive method that could be used for the synthesis of these electrode materials. Direct electrodeposition allows for excellent electrical contact to the current collector without the use of a binder. To successfully electrodeposit zinc and manganese antimonides, metal precursors with excellent solubility in water were needed. To promote solubility, particularly for the antimony precursor, coordinating ligands were added to the deposition bath solutions. This work shows that the choice of coordinating ligand and metal-ligand speciation can alter both the electrochemistry and the film composition. This work focuses on the search for appropriate coordinating ligands, solution pH, and bath temperatures so that high quality films of ZnSb, MnSb, and Mn2Sb may be electrochemically deposited on a conducting substrate. Increasing use of natural resources for energy generation has driven research in the area of energy storage using superconducting materials. To meet energy storage needs the materials must have the following features: (i) safety, (ii) superconductivity at or above liquid nitrogen temperature (77 K), (iii) low cost manufacturing processes, and (iv) robustness. The search for materials that meet all of these criteria is on-going, specifically in the area of high temperature superconductivity. The precise mechanism of superconductivity is not known. A few theories explain some of the phenomenological aspects, but not all. In order to logically select and synthesize high temperature superconductors for industrial applications, the precise mechanism must first be elucidated. Additionally, a synthetic method that yields pure, high quality crystals is required because transition temperatures have been shown to vary depending on the preparation method due to impurities. Before measuring properties of superconductors, the development of a synthesis method that yields pure, high quality crystals is required. Most superconductors are synthesized using traditional solid state methods. This synthesis route precludes formation of kinetically stable phases. Low temperature synthesis is useful for probing thermodynamic verses kinetic stability of compounds as well as producing high quality single crystals. A novel low temperature hydrothermal synthesis of Sr-Ru-O compounds has been developed. These materials are important because of their interesting properties including superconductivity and ferromagnetism. Sr2RuO4 is particularly interesting as it is superconducting and isostructural to La2CuO 4, which is only superconducting when doped. Therefore, Sr2RuO 4 is a good choice for study of the mechanism of superconductivity. Additionally, new kinetically stable phases of the Sr-Ru-O family may be formed which may also be superconducting. Sr-Ru-O compounds were previously synthesized via the float zone method. There is one report of using hydrothermal synthesis, but the temperatures used were 480--630 °C. In general, hydrothermal methods are advantageous because of the potential for moderate temperatures and pressures to be used. Additionally, the reaction temperature, precursor choice, and reaction time can all be used to tune the composition and morphology of the product. Hydrothermal methods are inexpensive and a one-step synthesis which is very convenient to scale up for industrial application. This work shows how a hydrothermal method at temperatures between 140 °C and 210 °C was developed for the synthesis of the Sr-Ru-O family of compounds.

  15. High energy storage density over a broad temperature range in sodium bismuth titanate-based lead-free ceramics.

    PubMed

    Yang, Haibo; Yan, Fei; Lin, Ying; Wang, Tong; Wang, Fen

    2017-08-18

    A series of (1-x)Bi 0.48 La 0.02 Na 0.48 Li 0.02 Ti 0.98 Zr 0.02 O 3 -xNa 0.73 Bi 0.09 NbO 3 ((1-x)LLBNTZ-xNBN) (x = 0-0.14) ceramics were designed and fabricated using the conventional solid-state sintering method. The phase structure, microstructure, dielectric, ferroelectric and energy storage properties of the ceramics were systematically investigated. The results indicate that the addition of Na 0.73 Bi 0.09 NbO 3 (NBN) could decrease the remnant polarization (P r ) and improve the temperature stability of dielectric constant obviously. The working temperature range satisfying TCC 150  °C  ≤±15% of this work spans over 400 °C with the compositions of x ≥ 0.06. The maximum energy storage density can be obtained for the sample with x = 0.10 at room temperature, with an energy storage density of 2.04 J/cm 3 at 178 kV/cm. In addition, the (1-x)LLBNTZ-xNBN ceramics exhibit excellent energy storage properties over a wide temperature range from room temperature to 90 °C. The values of energy storage density and energy storage efficiency is 0.91 J/cm 3 and 79.51%, respectively, for the 0.90LLBNTZ-0.10NBN ceramic at the condition of 100 kV/cm and 90 °C. It can be concluded that the (1-x)LLBNTZ-xNBN ceramics are promising lead-free candidate materials for energy storage devices over a broad temperature range.

  16. Effects of several factors on the heat-shock-induced thermotolerance of Listeria monocytogenes.

    PubMed Central

    Pagán, R; Condón, S; Sala, F J

    1997-01-01

    The influence of the temperature at which Listeria monocytogenes had been grown (4 or 37 degrees C) on the response to heat shocks of different durations at different temperatures was investigated. For cells grown at 4 degrees C, the effect of storage, prior to and after heat shock, on the induced thermotolerance was also studied. Death kinetics of heat-shocked cells is also discussed. For L. monocytogenes grown at 37 degrees C, the greatest response to heat shock was a fourfold increase in thermotolerance. For L. monocytogenes grown at 4 degrees C, the greatest response to heat shock was a sevenfold increase in thermotolerance. The only survival curves of cells to have shoulders were those for cells that had been heat shocked. A 3% concentration of sodium chloride added to the recovery medium made these shoulders disappear and decreased decimal reduction times. The percentage of cells for which thermotolerance increased after a heat shock was smaller the milder the heat shock and the longer the prior storage. PMID:9251209

  17. Hyperbaric storage of melon juice at and above room temperature and comparison with storage at atmospheric pressure and refrigeration.

    PubMed

    Queirós, Rui P; Santos, Mauro D; Fidalgo, Liliana G; Mota, Maria J; Lopes, Rita P; Inácio, Rita S; Delgadillo, Ivonne; Saraiva, Jorge A

    2014-03-15

    Hyperbaric storage (8h) of melon juice (a highly perishable food) at 25, 30 and 37°C, under pressure at 25-150 MPa was compared with atmospheric pressure storage (0.1 MPa) at the same temperatures and under refrigeration (4°C). Comparatively to the refrigerated condition, hyperbaric storage at 50/75 MPa resulted in similar or lower microbial counts (total aerobic mesophiles, enterobacteriaceae, and yeasts/moulds) while at 100/150 MPa, the counts were lower for all the tested temperatures, indicating in the latter case, in addition to microbial growth inhibition, a microbial inactivation effect. At 25 MPa no microbial inhibition was observed. Physicochemical parameters of all samples stored under pressure (pH, titratable acidity, total soluble solids, browning degree and cloudiness) did not show a clear variation trend with pressure, being the results globally similar to refrigeration storage. These results show the potential of hyperbaric storage, at and above room temperature and with potential energy savings, comparatively to refrigeration. Copyright © 2013 Elsevier Ltd. All rights reserved.

  18. Effects of room temperature aging on two cryogenic temperature sensor models used in aerospace applications

    NASA Astrophysics Data System (ADS)

    Courts, S. Scott; Krause, John

    2012-06-01

    Cryogenic temperature sensors used in aerospace applications are typically procured far in advance of the mission launch date. Depending upon the program, the temperature sensors may be stored at room temperature for extended periods as installation and groundbased testing can take years before the actual flight. The effects of long term storage at room temperature are sometimes approximated by the use of accelerated aging at temperatures well above room temperature, but this practice can yield invalid results as the sensing material and/or electrical contacting method can be increasingly unstable with higher temperature exposure. To date, little data are available on the effects of extended room temperature aging on sensors commonly used in aerospace applications. This research examines two such temperature sensors models - the Lake Shore Cryotronics, Inc. model CernoxTM and DT-670-SD temperature sensors. Sample groups of each model type have been maintained for ten years or longer with room temperature storage between calibrations. Over an eighteen year period, the CernoxTM temperature sensors exhibited a stability of better than ±20 mK for T<30 K and better than ±0.1% of temperature for T>30 K. Over a ten year period the model DT-670-SD sensors exhibited a stability of better than ±140 mK for T<25 K and better than ±75 mK for T>25 K.

  19. Heat transfer characteristics of coconut oil as phase change material to room cooling application

    NASA Astrophysics Data System (ADS)

    Irsyad, M.; Harmen

    2017-03-01

    Thermal comfort in a room is one of human needs in the workplace and dwellings, so that the use of air conditioning system in tropical countries is inevitable. This equipment has an impact on the increase of energy consumption. One method of minimizing the energy use is by using the phase change material (PCM) as thermal energy storage. This material utilizes the temperature difference between day and night for the storage and release of thermal energy. PCM development on application as a material for air cooling inlet, partitioning and interior needs to be supported by the study of heat transfer characteristics when PCM absorbs heat from ambient temperature. This study was conducted to determine the heat transfer characteristics on coconut oil as a phase change material. There are three models of experiments performed in this research. Firstly, an experiment was conducted to analyze the time that was needed by material to phase change by varying the temperature. The second experiment analyzed the heat transfer characteristics of air to PCM naturally convection. The third experiment analyzed the forced convection heat transfer on the surface of the PCM container by varying the air velocity. The data of experimental showed that, increasing ambient air temperature resulted in shorter time for phase change. At temperatures of 30°C, the time for phase change of PCM with the thickness of 8 cm was 1700 min, and it was stable at temperatures of 27°C. Increasing air temperature accelerated the phase change in the material. While for the forced convection heat transfer, PCM could reduce the air temperature in the range of 30 to 35°C at about 1 to 2°C, with a velocity of 1-3 m/s.

  20. Thermodynamics and performance of the Mg-H-F system for thermochemical energy storage applications.

    PubMed

    Tortoza, Mariana S; Humphries, Terry D; Sheppard, Drew A; Paskevicius, Mark; Rowles, Matthew R; Sofianos, M Veronica; Aguey-Zinsou, Kondo-Francois; Buckley, Craig E

    2018-01-24

    Magnesium hydride (MgH 2 ) is a hydrogen storage material that operates at temperatures above 300 °C. Unfortunately, magnesium sintering occurs above 420 °C, inhibiting its application as a thermal energy storage material. In this study, the substitution of fluorine for hydrogen in MgH 2 to form a range of Mg(H x F 1-x ) 2 (x = 1, 0.95, 0.85, 0.70, 0.50, 0) composites has been utilised to thermodynamically stabilise the material, so it can be used as a thermochemical energy storage material that can replace molten salts in concentrating solar thermal plants. These materials have been studied by in situ synchrotron X-ray diffraction, differential scanning calorimetry, thermogravimetric analysis, temperature-programmed-desorption mass spectrometry and Pressure-Composition-Isothermal (PCI) analysis. Thermal analysis has determined that the thermal stability of Mg-H-F solid solutions increases proportionally with fluorine content, with Mg(H 0.85 F 0.15 ) 2 having a maximum rate of H 2 desorption at 434 °C, with a practical hydrogen capacity of 4.6 ± 0.2 wt% H 2 (theoretical 5.4 wt% H 2 ). An extremely stable Mg(H 0.43 F 0.57 ) 2 phase is formed upon the decomposition of each Mg-H-F composition of which the remaining H 2 is not released until above 505 °C. PCI measurements of Mg(H 0.85 F 0.15 ) 2 have determined the enthalpy (ΔH des ) to be 73.6 ± 0.2 kJ mol -1 H 2 and entropy (ΔS des ) to be 131.2 ± 0.2 J K -1 mol -1 H 2 , which is slightly lower than MgH 2 with ΔH des of 74.06 kJ mol -1 H 2 and ΔS des = 133.4 J K -1 mol -1 H 2 . Cycling studies of Mg(H 0.85 F 0.15 ) 2 over six absorption/desorption cycles between 425 and 480 °C show an increased usable cycling temperature of ∼80 °C compared to bulk MgH 2 , increasing the thermal operating temperatures for technological applications.

  1. Effects of semen storage and separation techniques on sperm DNA fragmentation.

    PubMed

    Jackson, Robert E; Bormann, Charles L; Hassun, Pericles A; Rocha, André M; Motta, Eduardo L A; Serafini, Paulo C; Smith, Gary D

    2010-12-01

    To determine the effect of semen storage and separation techniques on sperm DNA fragmentation. Controlled clinical study. An assisted reproductive technology laboratory. Thirty normoozospermic semen samples obtained from patients undergoing infertility evaluation. One aliquot from each sample was immediately prepared (control) for the sperm chromatin dispersion assay (SCD). Aliquots used to assess storage techniques were treated in the following ways: snap frozen by liquid nitrogen immersion, slow frozen with Tris-yolk buffer and glycerol, kept on ice for 24 hours or maintained at room temperature for 4 and 24 hours. Aliquots used to assess separation techniques were processed by the following methods: washed and centrifuged in media, swim-up from washed sperm pellet, density gradient separation, density gradient followed by swim-up. DNA integrity was then measured by SCD. DNA fragmentation as measured by SCD. There was no significant difference in fragmentation among the snap frozen, slow frozen, and wet-ice groups. Compared to other storage methods short-term storage at room temperature did not impact DNA fragmentation yet 24 hours storage significantly increased fragmentation. Swim-up, density gradient and density gradient/swim-up had significantly reduced DNA fragmentation levels compared with washed semen. Postincubation, density gradient/swim-up showed the lowest fragmentation levels. The effect of sperm processing methods on DNA fragmentation should be considered when selecting storage or separation techniques for clinical use. Copyright © 2010 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.

  2. Advanced Thermal Storage for Central Receivers with Supercritical Coolants

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Kelly, Bruce D.

    2010-06-15

    The principal objective of the study is to determine if supercritical heat transport fluids in a central receiver power plant, in combination with ceramic thermocline storage systems, offer a reduction in levelized energy cost over a baseline nitrate salt concept. The baseline concept uses a nitrate salt receiver, two-tank (hot and cold) nitrate salt thermal storage, and a subcritical Rankine cycle. A total of 6 plant designs were analyzed, as follows: Plant Designation Receiver Fluid Thermal Storage Rankine Cycle Subcritical nitrate salt Nitrate salt Two tank nitrate salt Subcritical Supercritical nitrate salt Nitrate salt Two tank nitrate salt Supercritical Lowmore » temperature H2O Supercritical H2O Two tank nitrate salt Supercritical High temperature H2O Supercritical H2O Packed bed thermocline Supercritical Low temperature CO2 Supercritical CO2 Two tank nitrate salt Supercritical High temperature CO2 Supercritical CO2 Packed bed thermocline Supercritical Several conclusions have been drawn from the results of the study, as follows: 1) The use of supercritical H2O as the heat transport fluid in a packed bed thermocline is likely not a practical approach. The specific heat of the fluid is a strong function of the temperatures at values near 400 °C, and the temperature profile in the bed during a charging cycle is markedly different than the profile during a discharging cycle. 2) The use of supercritical CO2 as the heat transport fluid in a packed bed thermocline is judged to be technically feasible. Nonetheless, the high operating pressures for the supercritical fluid require the use of pressure vessels to contain the storage inventory. The unit cost of the two-tank nitrate salt system is approximately $24/kWht, while the unit cost of the high pressure thermocline system is nominally 10 times as high. 3) For the supercritical fluids, the outer crown temperatures of the receiver tubes are in the range of 700 to 800 °C. At temperatures of 700 °C and above, intermetallic compounds can precipitate between, and within, the grains of nickel alloys. The precipitation leads to an increase in tensile strength, and a decrease in ductility. Whether the proposed tube materials can provide the required low cycle fatigue life for the supercritical H2O and CO2 receivers is an open question. 4) A ranking of the plants, in descending order of technical and economic feasibility, is as follows: i) Supercritical nitrate salt and baseline nitrate salt: equal ratings ii) Low temperature supercritical H2O iii) Low temperature supercritical CO2 iv) High temperature supercritical CO2 v) High temperature supercritical H2O 5) The two-tank nitrate salt thermal storage systems are strongly preferred over the thermocline systems using supercritical heat transport fluids.« less

  3. CTC-mRNA (AR-V7) Analysis from Blood Samples—Impact of Blood Collection Tube and Storage Time

    PubMed Central

    Luk, Alison W. S.; Ma, Yafeng; Ding, Pei N.; Young, Francis P.; Chua, Wei; Balakrishnar, Bavanthi; Dransfield, Daniel T.; de Souza, Paul; Becker, Therese M.

    2017-01-01

    Circulating tumour cells (CTCs) are an emerging resource for monitoring cancer biomarkers. New technologies for CTC isolation and biomarker detection are increasingly sensitive, however, the ideal blood storage conditions to preserve CTC-specific mRNA biomarkers remains undetermined. Here we tested the preservation of tumour cells and CTC-mRNA over time in common anticoagulant ethylene-diamine-tetra-acetic acid (EDTA) and acid citrate dextrose solution B (Citrate) blood tubes compared to preservative-containing blood tubes. Blood samples spiked with prostate cancer cells were processed after 0, 24, 30, and 48 h storage at room temperature. The tumour cell isolation efficiency and the mRNA levels of the prostate cancer biomarkers androgen receptor variant 7 (AR-V7) and total AR, as well as epithelial cell adhesion molecule (EpCAM) were measured. Spiked cells were recovered across all storage tube types and times. Surprisingly, tumour mRNA biomarkers were readily detectable after 48 h storage in EDTA and Citrate tubes, but not in preservative-containing tubes. Notably, AR-V7 expression was detected in prostate cancer patient blood samples after 48 h storage in EDTA tubes at room temperature. This important finding presents opportunities for measuring AR-V7 expression from clinical trial patient samples processed within 48 h—a much more feasible timeframe compared to previous recommendations. PMID:28498319

  4. Quantifying the spoilage and shelf-life of yoghurt with fruits.

    PubMed

    Mataragas, M; Dimitriou, V; Skandamis, P N; Drosinos, E H

    2011-05-01

    The aim of the present study was to develop a predictive model to quantify the spoilage of yoghurt with fruits. Product samples were stored at various temperatures (5-20 °C). Samples were subjected to microbiological (total viable counts, lactic acid bacteria-LAB, yeasts and moulds) and physico-chemical analysis (pH, titratable acidity and sugars). LAB was the dominant micro-flora. Yeasts population increased at all temperatures but a delay was observed during the first days of storage. Titratable acidity and pH remained almost constant at low temperatures (5 and 10 °C). However, at higher temperatures (>10 °C), an increase in titratable acidity and reduction in pH was observed. Sugar concentration (fructose, lactose and glucose) decreased during storage. A mathematical model was developed for shelf-life determination of the product. It was successfully validated at a temperature (17 °C) not used during model development. The results showed that shelf-life of this product could not be established based only on microbiological data and use of other parameters such as sensory or/and physico-chemical analysis is required. Shelf-life determination by spoilage tests is time-consuming and the need for new rapid techniques has been raised. The developed model could help dairy industries to establish shelf-life predictions on yoghurt with fruits stored under constant temperature conditions. Copyright © 2010 Elsevier Ltd. All rights reserved.

  5. Self-Protection of Electrochemical Storage Devices via a Thermal Reversible Sol-Gel Transition.

    PubMed

    Yang, Hui; Liu, Zhiyuan; Chandran, Bevita K; Deng, Jiyang; Yu, Jiancan; Qi, Dianpeng; Li, Wenlong; Tang, Yuxin; Zhang, Chenguang; Chen, Xiaodong

    2015-10-07

    Thermal self-protected intelligent electrochemical storage devices are fabricated using a reversible sol-gel transition of the electrolyte, which can decrease the specific capacitance and increase and enable temperature-dependent charging and discharging rates in the device. This work represents proof of a simple and useful concept, which shows tremendous promise for the safe and controlled power delivery in electrochemical devices. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  6. Growth kinetics of Staphylococcus aureus on Brie and Camembert cheeses.

    PubMed

    Lee, Heeyoung; Kim, Kyungmi; Lee, Soomin; Han, Minkyung; Yoon, Yohan

    2014-05-01

    In this study, we developed mathematical models to describe the growth kinetics of Staphylococcus aureus on natural cheeses. A five-strain mixture of Staph. aureus was inoculated onto 15 g of Brie and Camembert cheeses at 4 log CFU/g. The samples were then stored at 4, 10, 15, 25, and 30 °C for 2-60 d, with a different storage time being used for each temperature. Total bacterial and Staph. aureus cells were enumerated on tryptic soy agar and mannitol salt agar, respectively. The Baranyi model was fitted to the growth data of Staph. aureus to calculate kinetic parameters such as the maximum growth rate in log CFU units (r max; log CFU/g/h) and the lag phase duration (λ; h). The effects of temperature on the square root of r max and on the natural logarithm of λ were modelled in the second stage (secondary model). Independent experimental data (observed data) were compared with prediction and the respective root mean square error compared with the RMSE of the fit on the original data, as a measure of model performance. The total growth of bacteria was observed at 10, 15, 25, and 30 °C on both cheeses. The r max values increased with storage temperature (P<0·05), but a significant effect of storage temperature on λ values was only observed between 4 and 15 °C (P<0·05). The square root model and linear equation were found to be appropriate for description of the effect of storage temperature on growth kinetics (R 2=0·894-0·983). Our results indicate that the models developed in this study should be useful for describing the growth kinetics of Staph. aureus on Brie and Camembert cheeses.

  7. Power-to-heat in adiabatic compressed air energy storage power plants for cost reduction and increased flexibility

    NASA Astrophysics Data System (ADS)

    Dreißigacker, Volker

    2018-04-01

    The development of new technologies for large-scale electricity storage is a key element in future flexible electricity transmission systems. Electricity storage in adiabatic compressed air energy storage (A-CAES) power plants offers the prospect of making a substantial contribution to reach this goal. This concept allows efficient, local zero-emission electricity storage on the basis of compressed air in underground caverns. The compression and expansion of air in turbomachinery help to balance power generation peaks that are not demand-driven on the one hand and consumption-induced load peaks on the other. For further improvements in cost efficiencies and flexibility, system modifications are necessary. Therefore, a novel concept regarding the integration of an electrical heating component is investigated. This modification allows increased power plant flexibilities and decreasing component sizes due to the generated high temperature heat with simultaneously decreasing total round trip efficiencies. For an exemplarily A-CAES case simulation studies regarding the electrical heating power and thermal energy storage sizes were conducted to identify the potentials in cost reduction of the central power plant components and the loss in round trip efficiency.

  8. Effects of holding temperature and storage time on respiratory rate, motility, and fertility of chicken and turkey semen.

    PubMed

    Clarke, R N; Sexton, T J; Ottinger, M A

    1982-09-01

    A series of experiments was conducted to measure the respiratory activity, motility, and fertility of chicken and turkey sperm in undiluted and diluted (1 part semen to 5 parts Beltsville Poultry Semen Extender) semen held at either 41, 25, 15, or 5 C for 3 or 6 hr. Sperm respiration was temperature dependent, increasing with increasing temperature. When incubated under identical conditions, the respiratory rate of spermatozoa in diluted semen of both species was significantly (P less than .05) higher than in undiluted semen. In undiluted and diluted chicken and turkey semen, sperm mortality was lowest at 41 C. No differences in the motility of spermatozoa from undiluted and diluted semen of either species were observed in the unstored controls. Sperm motility in samples held at 15 or 5 C was similar to that of the unstored controls. The fertility of diluted chicken semen was highest (75%) after 6 hr of storage at 5 C. Fertility levels (71 to 83%) of chicken semen was highest (75%) after 6 hr of storage at 5 C. Fertility levels (71 to 83%) of chicken semen held for 3 hr at 25, 15, or 5 C did not differ from the unstored control. In contrast, the fertilizing capacity of diluted turkey semen (0 to 45%) was significantly lower than the unstored controls (68%), regardless of holding times and temperatures. The fertility of undiluted turkey semen was significantly (P less than .05) lower than the unstored control in all cases, with the exception of semen held at 25 C for 3 hr.

  9. The use of salinity contrast for density difference compensation to improve the thermal recovery efficiency in high-temperature aquifer thermal energy storage systems

    NASA Astrophysics Data System (ADS)

    van Lopik, Jan H.; Hartog, Niels; Zaadnoordijk, Willem Jan

    2016-08-01

    The efficiency of heat recovery in high-temperature (>60 °C) aquifer thermal energy storage (HT-ATES) systems is limited due to the buoyancy of the injected hot water. This study investigates the potential to improve the efficiency through compensation of the density difference by increased salinity of the injected hot water for a single injection-recovery well scheme. The proposed method was tested through numerical modeling with SEAWATv4, considering seasonal HT-ATES with four consecutive injection-storage-recovery cycles. Recovery efficiencies for the consecutive cycles were investigated for six cases with three simulated scenarios: (a) regular HT-ATES, (b) HT-ATES with density difference compensation using saline water, and (c) theoretical regular HT-ATES without free thermal convection. For the reference case, in which 80 °C water was injected into a high-permeability aquifer, regular HT-ATES had an efficiency of 0.40 after four consecutive recovery cycles. The density difference compensation method resulted in an efficiency of 0.69, approximating the theoretical case (0.76). Sensitivity analysis showed that the net efficiency increase by using the density difference compensation method instead of regular HT-ATES is greater for higher aquifer hydraulic conductivity, larger temperature difference between injection water and ambient groundwater, smaller injection volume, and larger aquifer thickness. This means that density difference compensation allows the application of HT-ATES in thicker, more permeable aquifers and with larger temperatures than would be considered for regular HT-ATES systems.

  10. Separators - Technology review: Ceramic based separators for secondary batteries

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Nestler, Tina; Schmid, Robert; Münchgesang, Wolfram

    Besides a continuous increase of the worldwide use of electricity, the electric energy storage technology market is a growing sector. At the latest since the German energy transition ('Energiewende') was announced, technological solutions for the storage of renewable energy have been intensively studied. Storage technologies in various forms are commercially available. A widespread technology is the electrochemical cell. Here the cost per kWh, e. g. determined by energy density, production process and cycle life, is of main interest. Commonly, an electrochemical cell consists of an anode and a cathode that are separated by an ion permeable or ion conductive membranemore » - the separator - as one of the main components. Many applications use polymeric separators whose pores are filled with liquid electrolyte, providing high power densities. However, problems arise from different failure mechanisms during cell operation, which can affect the integrity and functionality of these separators. In the case of excessive heating or mechanical damage, the polymeric separators become an incalculable security risk. Furthermore, the growth of metallic dendrites between the electrodes leads to unwanted short circuits. In order to minimize these risks, temperature stable and non-flammable ceramic particles can be added, forming so-called composite separators. Full ceramic separators, in turn, are currently commercially used only for high-temperature operation systems, due to their comparably low ion conductivity at room temperature. However, as security and lifetime demands increase, these materials turn into focus also for future room temperature applications. Hence, growing research effort is being spent on the improvement of the ion conductivity of these ceramic solid electrolyte materials, acting as separator and electrolyte at the same time. Starting with a short overview of available separator technologies and the separator market, this review focuses on ceramic-based separators. Two prominent examples, the lithium-ion and sodium-sulfur battery, are described to show the current stage of development. New routes are presented as promising technologies for safe and long-life electrochemical storage cells.« less

  11. Separators - Technology review: Ceramic based separators for secondary batteries

    NASA Astrophysics Data System (ADS)

    Nestler, Tina; Schmid, Robert; Münchgesang, Wolfram; Bazhenov, Vasilii; Schilm, Jochen; Leisegang, Tilmann; Meyer, Dirk C.

    2014-06-01

    Besides a continuous increase of the worldwide use of electricity, the electric energy storage technology market is a growing sector. At the latest since the German energy transition ("Energiewende") was announced, technological solutions for the storage of renewable energy have been intensively studied. Storage technologies in various forms are commercially available. A widespread technology is the electrochemical cell. Here the cost per kWh, e. g. determined by energy density, production process and cycle life, is of main interest. Commonly, an electrochemical cell consists of an anode and a cathode that are separated by an ion permeable or ion conductive membrane - the separator - as one of the main components. Many applications use polymeric separators whose pores are filled with liquid electrolyte, providing high power densities. However, problems arise from different failure mechanisms during cell operation, which can affect the integrity and functionality of these separators. In the case of excessive heating or mechanical damage, the polymeric separators become an incalculable security risk. Furthermore, the growth of metallic dendrites between the electrodes leads to unwanted short circuits. In order to minimize these risks, temperature stable and non-flammable ceramic particles can be added, forming so-called composite separators. Full ceramic separators, in turn, are currently commercially used only for high-temperature operation systems, due to their comparably low ion conductivity at room temperature. However, as security and lifetime demands increase, these materials turn into focus also for future room temperature applications. Hence, growing research effort is being spent on the improvement of the ion conductivity of these ceramic solid electrolyte materials, acting as separator and electrolyte at the same time. Starting with a short overview of available separator technologies and the separator market, this review focuses on ceramic-based separators. Two prominent examples, the lithium-ion and sodium-sulfur battery, are described to show the current stage of development. New routes are presented as promising technologies for safe and long-life electrochemical storage cells.

  12. Local structure of Ge2Sb2Te5 during crystallization under pressure

    NASA Astrophysics Data System (ADS)

    Roscioni, O. M.; Branicio, P. S.; Kalikka, J.; Zhou, X.; Simpson, R. E.

    2018-04-01

    The role of stress on the crystallization process of the phase change data storage material, Ge2Sb2Te5, is studied. When thin Ge2Sb2Te5 films are capped with Si3N4, stress is generated in the Ge2Sb2Te5 layer which causes the crystallization temperature to increase. Si3N4 films of 25 nm thickness increase the crystallization temperature from 446 K to 464 K. We show that stress predominantly destabilizes voids and increases the number of Ge-Sb and homopolar bonds in the vicinity of Ge atoms, and this makes the crystallization less probable, thus resulting in the increase in the measured temperature.

  13. Coupled thermal-hydrological-mechanical behavior of rock mass surrounding a high-temperature thermal energy storage cavern at shallow depth

    DOE PAGES

    Park, Jung-Wook; Rutqvist, Jonny; Ryu, Dongwoo; ...

    2016-01-15

    The present study is aimed at numerically examining the thermal-hydrological-mechanical (THM) processes within the rock mass surrounding a cavern used for thermal energy storage (TES). We considered a cylindrical rock cavern with a height of 50 m and a radius of 10 m storing thermal energy of 350ºC as a conceptual TES model and simulated its operation for 30 years using THM coupled numerical modeling. At first, the insulator performance was not considered for the purpose of investigating the possible coupled THM behavior of the surrounding rock mass; then, the effects of an insulator were examined for different insulator thicknesses.more » The key concerns were focused on the hydro-thermal multiphase flow and heat transport in the rock mass around the thermal storage cavern, the effect of evaporation of rock mass, thermal impact on near the ground surface and the mechanical behavior of the surrounding rock mass. It is shown that the rock temperature around the cavern rapidly increased in the early stage and, consequently, evaporation of groundwater occurred, raising the fluid pressure. However, evaporation and multiphase flow did not have a significant effect on the heat transfer and mechanical behavior in spite of the high-temperature (350ºC) heat source. The simulations showed that large-scale heat flow around a cavern was expected to be conductiondominated for a reasonable value of rock mass permeability. Thermal expansion as a result of the heating of the rock mass from the storage cavern led to a ground surface uplift on the order of a few centimeters and to the development of tensile stress above the storage cavern, increasing the potentials for shear and tensile failures after a few years of the operation. Finally, the analysis showed that high tangential stress in proximity of the storage cavern can some shear failure and local damage, although large rock wall failure could likely be controlled with appropriate insulators and reinforcement.« less

  14. Storage at low temperature differentially affects the colour and carotenoid composition of two cultivars of banana.

    PubMed

    Facundo, Heliofabia Virginia De Vasconcelos; Gurak, Poliana Deyse; Mercadante, Adriana Zerlotti; Lajolo, Franco Maria; Cordenunsi, Beatriz Rosana

    2015-03-01

    Different storage conditions can induce changes in the colour and carotenoid profiles and levels in some fruits. The goal of this work was to evaluate the influence of low temperature storage on the colour and carotenoid synthesis in two banana cultivars: Prata and Nanicão. For this purpose, the carotenoids from the banana pulp were determined by HPLC-DAD-MS/MS, and the colour of the banana skin was determined by a colorimeter method. Ten carotenoids were identified, of which the major carotenoids were all-trans-lutein, all-trans-α-carotene and all-trans-β-carotene in both cultivars. The effect of the low temperatures was subjected to linear regression analysis. In cv. Prata, all-trans-α-carotene and all-trans-β-carotene were significantly affected by low temperature (p<0.01), with negative estimated values (β coefficients) indicating that during cold storage conditions, the concentrations of these carotenoids tended to decrease. In cv. Nanicão, no carotenoid was significantly affected by cold storage (p>0.05). The accumulation of carotenoids in this group may be because the metabolic pathways using these carotenoids were affected by storage at low temperatures. The colour of the fruits was not negatively affected by the low temperatures (p>0.05). Published by Elsevier Ltd.

  15. Room-temperature storage of medications labeled for refrigeration.

    PubMed

    Cohen, Victor; Jellinek, Samantha P; Teperikidis, Leftherios; Berkovits, Elliot; Goldman, William M

    2007-08-15

    Data regarding the recommended maximum duration that refrigerated medications available in hospital pharmacies may be stored safely at room temperature were collected and compiled in a tabular format. During May and June of 2006, the prescribing information for medications labeled for refrigeration as obtained from the supplier were reviewed for data addressing room-temperature storage. Telephone surveys of the products' manufacturers were conducted when this information was not available in the prescribing information. Medications were included in the review if they were labeled to be stored at 2-8 degrees C and purchased by the pharmacy department for uses indicated on the hospital formulary. Frozen antibiotics thawed in the refrigerator and extemporaneously compounded medications were excluded. Information was compiled and arranged in tabular format. The U.S. Pharmacopeia's definition of room temperature (20-25 degrees C [68-77 degrees F]) was used for this review. Of the 189 medications listed in AHFS Drug Information 2006 for storage in a refrigerator, 89 were present in the pharmacy department's refrigerator. Since six manufacturers were unable to provide information for 10 medications, only 79 medications were included in the review. This table may help to avoid unnecessary drug loss and expenditures due to improper storage temperatures. Information regarding the room-temperature storage of 79 medications labeled for refrigerated storage was compiled.

  16. Moisture plasticization for enteric Eudragit® L30D-55-coated pellets prior to compression into tablets.

    PubMed

    Rujivipat, Soravoot; Bodmeier, Roland

    2012-05-01

    Enteric polymers such as cellulose esters (cellulose acetate phthalate, hydroxypropylmethylcellulose acetate succinate) and methacrylic acid-acrylate copolymers (Eudragit® L100-55 and S100) are quite brittle in the dry state and thus not suitable as pellet coatings for compression into tablets. The objective of this study was to investigate the role of humidity treatment for moisture plasticization in order to successfully compress the enterically coated pellets. The mechanical properties of Eudragit® L100-55 improved dramatically, while the properties of the other enteric polymers showed only minor changes after storage at higher humidity. The significant increase in flexibility of the Eudragit® L film was caused by hydration/plasticization; its elongation value changed from approx. 3% in the dry state to approx. 140% at the higher storage humidity. Storage at 84% relative humidity resulted in comparable release profiles of compressed and uncompressed pellets. The glass transition temperature of Eudragit® L films decreased below the compression temperature (room temperature) at storage humidities between 75% and 84%. The glass transition relative humidity leading to a change from the glassy to the rubbery state was determined by dynamic vapor sorption (DVS) to be 76.8%. Moisture resulted in superior plasticization for Eudragit® L than the conventional plasticizer triethyl citrate. The improved compressibility of high humidity treated Eudragit® L-coated pellets was also shown with single pellet compression data as indicated by an increased crushing force and deformation. In conclusion, moisture plasticization was a highly effective tool to enable the successful compression of pellets coated with the brittle enteric polymer Eudragit® L. Copyright © 2012 Elsevier B.V. All rights reserved.

  17. Water quality problems associated with intermittent water supply.

    PubMed

    Tokajian, S; Hashwa, F

    2003-01-01

    A controlled study was conducted in Lebanon over a period of 12 months to determine bacterial regrowth in a small network supplying the Beirut suburb of Naccache that had a population of about 3,000. The residential area, which is fed by gravity, is supplied twice a week with chlorinated water from two artesian wells of a confined aquifer. A significant correlation was detected between the turbidity and the levels of heterotrophic plate count bacteria (HPC) in the samples from the distribution network as well as from the artesian wells. However, a negative significant correlation was found between the temperature and the HPC count in the samples collected from the source. A statistically significant increase in counts, possibly due to regrowth, was repeatedly established between two sampling points lying on a straight distribution line but 1 km apart. Faecal coliforms were detected in the source water but none in the network except during a pipe breakage incident with confirmed Escherichia coli reaching 40 CFU/100 mL. However, coliforms such as Citrobacter freundii, Enterobacter agglomerans, E. cloacae and E. skazakii were repeatedly isolated from the network, mainly due to inadequate chlorination. A second controlled study was conducted to determine the effect of storage on the microbial quality of household storage tanks (500 L), which were of two main types - galvanized cast iron and black polyethylene. The mean bacterial count increased significantly after 7 d storage in both tank types. A significant difference was found in the mean HPC/mL between the winter and the summer. Highest counts were found April-June although the maximum temperature was reported later in the summer. A positive correlation was established between the HPC/mL and pH, temperature and storage time.

  18. Effect of sand and method of mixing on molten salt properties for an open direct absorption solar receiver/storage system

    NASA Astrophysics Data System (ADS)

    AlQaydi, Muna; Delclos, Thomas; AlMheiri, Saif; Calvet, Nicolas

    2017-06-01

    The concept of CSPonD Demo project is based on a single and open molten salt tank as a thermal solar receiver and storage unit. Therefore, the effect of external environment such as sand and air on the thermophysical properties of nitrate salt (60 wt. % sodium nitrate, 40 wt. % potassium nitrate) has been investigated in this work. Differential Scanning Calorimeter (DSC) was used to determine the melting, solidification temperatures while the thermal stability and mass loss measurements were carried on Thermal Gravimetric Analysis (TGA). Measurements under nitrogen indicate that the adding 2% (w/w) sand has negative impact by increasing the solidification temperature, mass loss percentage and decreasing the stability limit. While the melting temperature was not affected by the sand and by the preparation method. On the other hand, measurement under air showed an increase of the stability limit and decrease of the mass loss percentage. Furthermore, the measurements for the mass loss under air did not reach a stable value, which required further investigation.

  19. A comparative analysis of the cryo-compression and cryo-adsorption hydrogen storage methods

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Petitpas, G; Benard, P; Klebanoff, L E

    2014-07-01

    While conventional low-pressure LH₂ dewars have existed for decades, advanced methods of cryogenic hydrogen storage have recently been developed. These advanced methods are cryo-compression and cryo-adsorption hydrogen storage, which operate best in the temperature range 30–100 K. We present a comparative analysis of both approaches for cryogenic hydrogen storage, examining how pressure and/or sorbent materials are used to effectively increase onboard H₂ density and dormancy. We start by reviewing some basic aspects of LH₂ properties and conventional means of storing it. From there we describe the cryo-compression and cryo-adsorption hydrogen storage methods, and then explore the relationship between them, clarifyingmore » the materials science and physics of the two approaches in trying to solve the same hydrogen storage task (~5–8 kg H₂, typical of light duty vehicles). Assuming that the balance of plant and the available volume for the storage system in the vehicle are identical for both approaches, the comparison focuses on how the respective storage capacities, vessel weight and dormancy vary as a function of temperature, pressure and type of cryo-adsorption material (especially, powder MOF-5 and MIL-101). By performing a comparative analysis, we clarify the science of each approach individually, identify the regimes where the attributes of each can be maximized, elucidate the properties of these systems during refueling, and probe the possible benefits of a combined “hybrid” system with both cryo-adsorption and cryo-compression phenomena operating at the same time. In addition the relationships found between onboard H₂ capacity, pressure vessel and/or sorbent mass and dormancy as a function of rated pressure, type of sorbent material and fueling conditions are useful as general designing guidelines in future engineering efforts using these two hydrogen storage approaches.« less

  20. Preliminary investigations on a new method of retaining the colour of shucked cockles ( Anadara Granosa), and the extension of shelflife by gamma irradiation and vacuum packing

    NASA Astrophysics Data System (ADS)

    Ng, Cher Siang; Low, Lai Kim; Chia, Lawrence H. L.

    Live cockles were incubated in atmospheres containing different concentrations of carbon monoxide. Since CO combines more readily with myoglobin and haemoglobin than oxygen, the formation of in vivo deoxygenated haemoglobins and post mortem formation of methaemoglobin were retarded by the more stable carboxyhaemoglobin (HbCO). The bright red colour of the stable HbCO is retained during storage, giving the desired colour to the cockles. The colour of normal, chilled cockle meat deteriorated after 3 days ice storage while those treated with 50 and 100% CO retained the bright deep orange colour up to 10 days storage. Irradiation caused faster colour deterioration in both CO and non-CO treated samples. Vacuum packing influenced the colour of the cockles with irradiation and with CO treatments. In non-CO treated, irradiated samples, the effect of vacuum packing was not obvious. In CO treated, irradiated samples, vacuum packing retarded the deterioration of colour. Odour developments were influenced by irradiation, vacuum packing and storage temperature, and were not influenced by CO treatments. Irradiation suppressed the development of odour for the first 11 days storage (0°C) while vacuum packing depressed the odour by lowering its intensity instead. Odour development was slowed down by lowering the storage temperature. The odour of shucked cockles was rejected within one day at room temperature (26-28°C) while at 0°C the odour of the shucked cockles was still acceptable after 10 days. Suitable chemical indices for quality are K value and TVBN. Treatment with CO did not influence the K value development. Vacuum packing produced the highest K values after 19 days storage (0°C), while irradiated samples had higher K values than non-irradiated samples. The TVBN increased with storage and is an indicator of the odour development. The use of CO treatment extended the shelflife of the cockles based on appearance. A combination of CO treatment, vacuum packing, ice storage and irradiation extended the shelflife to beyond 18 days, based on odour, colour, and overall appearance.

  1. Shelf-life of bioprosthetic heart valves: a structural and mechanical study.

    PubMed

    Julien, M; Létouneau, D R; Marois, Y; Cardou, A; King, M W; Guidoin, R; Chachra, D; Lee, J M

    1997-04-01

    This study was undertaken to evaluate the influence of storage conditions on the shelf-life of porcine bioprosthetic valves. Fifty-five unimplanted porcine bioprostheses have been evaluated. The valves were stored in 0.5% buffered glutaraldehyde solution for different periods of time (7, 23 and 32 months). Twenty-eight valves were refrigerated while the remaining valves were stored at room temperature. The pH of the glutaraldehyde solution at room temperature decreased with time of storage, while that kept in the refrigerator remained stable over the course of the study. Macroscopic observations showed that the valve tissues kept at room temperature, especially for the periods of 23 and 32 months, became darker and more yellow in colour, whereas the refrigerated specimens exhibited no such changes in appearance. Scanning electron microscopy analysis revealed no noticeable differences on the surfaces of the leaflets stored under different conditions. Mechanical tests, including stress-strain response, stress relaxation and fracture behaviour, were carried out. Analysis of variance showed that the storage temperature, but not the length of storage, had a significant effect on some mechanical properties. The stress relaxation at 1000 s (P = 0.05), the ultimate tensile strength (P = 0.01) and the strain at fracture (P = 0.04) were all higher after storage at room temperature compared to the results after refrigeration. No statistically significant changes in the denaturation temperature of the collagen were observed between the different storage conditions. In conclusion, the storage temperature appears to have some influence on the bioprosthetic tissue. The bioprostheses stored under ambient conditions experience changes which may influence their longterm in vivo performance.

  2. Climatic, biological, and land cover controls on the exchange of gas-phase semivolatile chemical pollutants between forest canopies and the atmosphere.

    PubMed

    Nizzetto, Luca; Perlinger, Judith A

    2012-03-06

    An ecophysiological model of a structured broadleaved forest canopy was coupled to a chemical fate model of the air-canopy exchange of gaseous semivolatile chemicals to dynamically assess the short-term (hours) and medium term (days to season) air-canopy exchange and the influence of biological, climatic, and land cover drivers on the dynamics of the air-canopy exchange and on the canopy storage for airborne semivolatile pollutants. The chemical fate model accounts for effects of short-term variations in air temperature, wind speed, stomatal opening, and leaf energy balance, all as a function of layer in the canopy. Simulations showed the potential occurrence of intense short/medium term re-emission of pollutants having log K(OA) up to 10.7 from the canopy as a result of environmental forcing. In addition, relatively small interannual variations in seasonally averaged air temperature, canopy biomass, and precipitation can produce relevant changes in the canopy storage capacity for the chemicals. It was estimated that possible climate change related variability in environmental parameters (e.g., an increase of 2 °C in seasonally averaged air temperature in combination with a 10% reduction in canopy biomass due to, e.g., disturbance or acclimatization) may cause a reduction in canopy storage capacity of up to 15-25%, favoring re-emission and potential for long-range atmospheric transport. On the other hand, an increase of 300% in yearly precipitation can increase canopy sequestration by 2-7% for the less hydrophobic compounds.

  3. Effects of storage conditions on hatchability, embryonic survival and cytoarchitectural properties in broiler from young and old flocks.

    PubMed

    Pokhrel, N; Cohen, E Ben-Tal; Genin, O; Ruzal, M; Sela-Donenfeld, D; Cinnamon, Y

    2018-04-01

    Storing eggs at low temperature prior to incubation is common practice in the broiler hatchery industry; however, prolonged storage (beyond 7 d) is known to increase early embryonic mortality and reduce chick quality and performance. To better understand the basis of this mortality, we previously published milestone criteria to evaluate morphological and cellular properties of the freshly laid embryo. Using these criteria, in the present study we checked the effects of storage at 18°C and 12°C for up to 28 d on hatchability and chick quality. Furthermore, using a 3D high-resolution episcopic microscopy (HREM) imaging system combined with standard and confocal microscopy and cell viability markers, we analyzed the effects of the different storage conditions on embryonic developmental stage, cytoarchitectural properties, mitotic index and cell survival. A total of 1,483 eggs from a young flock were divided in 2 groups, 18°C and 12°C, and stored for 7, 14, 21, and 28 d. Following storage, randomly selected 1,222 eggs were incubated, and the hatched chicks were evaluated for chick quality parameters. Nonhatched eggs were also analyzed to determine the stage of embryonic mortality. The remaining 261 eggs were isolated and analyzed for developmental stage, cytoarchitecture, mitotic index, and cell death following storage. Hatchability rates beyond 7 d of storage at 12°C were significantly improved compared to 18°C, and chick quality remained high. Similar results were obtained for an old flock's eggs (n = 1,350). Analyzing the embryos, at each time point, we found that at 12°C, the developmental progression during storage slows significantly, mitotic index-which at this temperature may indicate mitotic arrest-increases and the rate of early apoptosis is half than at 18°C. Moreover, the HREM system and histological sections showed that embryos stored at 18°C for prolonged times undergo dramatic cytoarchitectural changes that may be maladaptive to resuming normal development after diapause. We thus demonstrate the usefulness of the milestone criteria for predicting and studying the storage conditions that will allow for better performance in hatchery practice.

  4. Calendar aging of a graphite/LiFePO4 cell

    NASA Astrophysics Data System (ADS)

    Kassem, M.; Bernard, J.; Revel, R.; Pélissier, S.; Duclaud, F.; Delacourt, C.

    2012-06-01

    Graphite/LFP commercial cells are stored under 3 different conditions of temperature (30 °C, 45 °C, and 60 °C) and SOC (30%, 65%, and 100%) during up to 8 months. Several non-destructive electrochemical tests are performed at different storage times in order to understand calendar aging phenomena. After storage, all the cells except those stored at 30 °C exhibited capacity fade. The extent of capacity fade strongly increases with storage temperature and to a lesser extent with the state of charge. From in-depth data analysis, cyclable lithium loss was identified as the main source of capacity fade. This loss arises from side reactions taking place at the anode, e.g. solvent decomposition leading to the growth of the solid electrolyte interphase. However, the existence of reversible capacity loss also suggests the presence of side reactions occurring at the cathode, which are less prominent than those at the anode. The analyses do not show any evidence about active-material loss in the electrodes. The cells do not suffer substantial change in internal resistance. According to EIS analysis, the overall impedance increase is 70% or less.

  5. Evaluation of the performance characteristics of bilayer tablets: Part II. Impact of environmental conditions on the strength of bilayer tablets.

    PubMed

    Kottala, Niranjan; Abebe, Admassu; Sprockel, Omar; Bergum, James; Nikfar, Faranak; Cuitiño, Alberto M

    2012-12-01

    Ambient air humidity and temperature are known to influence the mechanical strength of tablets. The objective of this work is to understand the influence of processing parameters and environmental conditions (humidity and temperature) on the strength of bilayer tablets. As part of this study, bilayer tablets were compressed with different layer ratios, dwell times, layer sequences, material properties (plastic and brittle), first and second layer forces, and lubricant concentrations. Compressed tablets were stored in stability chambers controlled at predetermined conditions (40C/45%RH, 40C/75%RH) for 1, 3, and 5 days. The axial strength of the stored tablets was measured and a statistical model was developed to determine the effects of the aforementioned factors on the strength of bilayer tablets. As part of this endeavor, a full 3 × 2(4) factorial design was executed. Responses of the experiments were analyzed using PROC GLM of SAS (SAS Institute Inc, Cary, North Carolina, USA). A model was fit using all the responses to determine the significant interactions (p < 0.05). Results of this study indicated that storage conditions and storage time have significant impact on the strength of bilayer tablets. For Avicel-lactose and lactose-Avicel tablets, tablet strength decreased with the increasing humidity and storage time. But for lactose-lactose tablets, due to the formation of solid bridges upon storage, an increase in tablet strength was observed. Significant interactions were observed between processing parameters and storage conditions on the strength of bilayer tablets.

  6. The Effect of Storage and Extraction Methods on Amplification of Plasmodium falciparum DNA from Dried Blood Spots.

    PubMed

    Schwartz, Alanna; Baidjoe, Amrish; Rosenthal, Philip J; Dorsey, Grant; Bousema, Teun; Greenhouse, Bryan

    2015-05-01

    Extraction and amplification of DNA from dried blood spots (DBS) collected in field studies is commonly used for detection of Plasmodium falciparum. However, there have been few systematic efforts to determine the effects of storage and extraction methods on the sensitivity of DNA amplification. We investigated the effects of storage conditions, length of storage, and DNA extraction methods on amplification via three PCR-based assays using field samples and laboratory controls. Samples stored as DBS for 2 or more years at ambient temperature showed a significant loss of sensitivity that increased with time; after 10 years only 10% samples with parasite densities > 1,000 parasites/μL were detectable by nested polymerase chain reaction (PCR). Conversely, DBS and extracted DNA stored at -20°C showed no loss of sensitivity with time. Samples with low parasite densities amplified more successfully with saponin/Chelex compared with spin-column-based extraction, though the latter method performed better on samples with higher parasite densities stored for 2 years at ambient temperature. DNA extracted via both methods was stable after 20 freeze-thaw cycles. Our results suggest that DBS should be stored at -20°C or extracted immediately, especially if anticipating 2 or more years of storage. © The American Society of Tropical Medicine and Hygiene.

  7. The Effect of Storage and Extraction Methods on Amplification of Plasmodium falciparum DNA from Dried Blood Spots

    PubMed Central

    Schwartz, Alanna; Baidjoe, Amrish; Rosenthal, Philip J.; Dorsey, Grant; Bousema, Teun; Greenhouse, Bryan

    2015-01-01

    Extraction and amplification of DNA from dried blood spots (DBS) collected in field studies is commonly used for detection of Plasmodium falciparum. However, there have been few systematic efforts to determine the effects of storage and extraction methods on the sensitivity of DNA amplification. We investigated the effects of storage conditions, length of storage, and DNA extraction methods on amplification via three PCR-based assays using field samples and laboratory controls. Samples stored as DBS for 2 or more years at ambient temperature showed a significant loss of sensitivity that increased with time; after 10 years only 10% samples with parasite densities > 1,000 parasites/μL were detectable by nested polymerase chain reaction (PCR). Conversely, DBS and extracted DNA stored at −20°C showed no loss of sensitivity with time. Samples with low parasite densities amplified more successfully with saponin/Chelex compared with spin-column-based extraction, though the latter method performed better on samples with higher parasite densities stored for 2 years at ambient temperature. DNA extracted via both methods was stable after 20 freeze-thaw cycles. Our results suggest that DBS should be stored at −20°C or extracted immediately, especially if anticipating 2 or more years of storage. PMID:25758652

  8. Temperature-Dependent Survival of Hepatitis A Virus during Storage of Contaminated Onions

    PubMed Central

    Sun, Y.; Laird, D. T.

    2012-01-01

    Pre- or postharvest contamination of green onions by hepatitis A virus (HAV) has been linked to large numbers of food-borne illnesses. Understanding HAV survival in onions would assist in projecting the risk of the disease associated with their consumption. This study defined HAV inactivation rates in contaminated green onions contained in air-permeable, moisture-retaining high-density polyethylene packages that were stored at 3, 10, 14, 20, 21, 22, and 23°C. A protocol was established to recover HAV from whole green onions, with 31% as the average recovery by infectivity assay. Viruses in eluates were primarily analyzed by a 6-well plaque assay on FRhK-4 cells. Eight storage trials, including two trials at 3°C, were conducted, with 3 to 7 onion samples per sampling and 4 to 7 samplings per trial. Linear regression correlation (r2 = 0.80 to 0.98) was observed between HAV survival and storage time for each of the 8 trials, held at specific temperatures. Increases in the storage temperature resulted in greater HAV inactivation rates, e.g., a reduction of 0.033 log PFU/day at 3.4 ± 0.3°C versus 0.185 log PFU/day at 23.4 ± 0.7°C. Thus, decimal reduction time (D) values of 30, 14, 11, and 5 days, respectively, were obtained for HAV in onions stored at 3, 10, 14, and 23°C. Further regression analysis determined that 1 degree Celsius increase would increase inactivation of HAV by 0.007 log PFU/day in onions (r2 = 0.97). The data suggest that natural degradation of HAV in contaminated fresh produce is minimal and that a preventive strategy is critical to produce safety. The results are useful in predicting the risks associated with HAV contamination in fresh produce. PMID:22544253

  9. Evaluation of Oxygen Concentrators and Chemical Oxygen Generators at Altitude and Temperature Extremes.

    PubMed

    Blakeman, Thomas C; Rodriquez, Dario; Britton, Tyler J; Johannigman, Jay A; Petro, Michael C; Branson, Richard D

    2016-05-01

    Oxygen cylinders are heavy and present a number of hazards, and liquid oxygen is too heavy and cumbersome to be used in far forward environments. Portable oxygen concentrators (POCs) and chemical oxygen generators (COGs) have been proposed as a solution. We evaluated 3 commercially available POCs and 3 COGs in a laboratory setting. Altitude testing was done at sea level and 8,000, 16,000, and 22,000 ft. Temperature extreme testing was performed after storing devices at 60°C and -35°C for 24 hours. Mean FIO2 decreased after storage at -35°C with Eclipse and iGo POCs and also at the higher volumes after storage at 60°C with the Eclipse. The iGo ceased to operate at 16,000 ft, but the Eclipse and Saros were unaffected by altitude. Oxygen flow, duration of operation, and total oxygen volume varied between COGs and within the same device type. Output decreased after storage at -35°C, but increased at each altitude as compared to sea level. This study showed significant differences in the performance of POCs and COGs after storage at temperature extremes and with the COGs at altitude. Clinicians must understand the performance characteristics of devices in all potential environments. Reprint & Copyright © 2016 Association of Military Surgeons of the U.S.

  10. Impact of Different Elastomer Formulations on Moisture Permeation through Stoppers Used for Lyophilized Products Stored under Humid Conditions.

    PubMed

    Sasaki, Hitoshi; Kikuchi, Jun; Maeda, Terutoshi; Kuboniwa, Hitoshi

    2010-01-01

    The purpose of this study was to evaluate the effect of moisture permeability of different elastomer formulation stoppers, which had different moisture absorption abilities, on the increase of moisture content inside lyophilized vials during long-term storage under humid conditions. Two different elastomer formulation stoppers (high-moisture and low-moisture uptake stoppers) were compared. The increased amount of moisture content inside lyophilized vials fitted with high-moisture stoppers was higher than those fitted with low-moisture stoppers during the early stage of storage. However, this trend was reversed during the later stage of storage. Our data show that the moisture increase inside the lyophilized vials at the early stage was caused by moisture transfer from the stoppers, whereas the later moisture increase was caused by external moisture permeation through the stoppers. Results indicate that the difference in the moisture uptake profile inside the lyophilized vials at each period of storage was caused by the moisture absorption ability and moisture permeation ability of the two elastomer formulation stoppers. In terms of long-term storage stability under humid conditions, our data indicate that external moisture permeating through the stopper into the lyophilized vial during the late stage was the more important factor. In addition, the increase in moisture content at the early stage was controlled by stopper drying time. Furthermore, stopper drying time did not have an effect on moisture permeation at the late stage. Moisture permeation during the storage period appears to be dependent on the different elastomer formulations of the stoppers. The moisture permeation of different elastomer stoppers was an important factor in terms of the increased moisture content inside the lyophilized vials during the late stage of long-term storage under humid conditions. For lyophilized products stored at room temperature, the moisture permeation ability of the stopper is one of the most important factors for long-term storage stability.

  11. On the Fracture Toughness and Crack Growth Resistance of Bio-Inspired Thermal Spray Hybrid Composites

    NASA Astrophysics Data System (ADS)

    Resnick, Michael Murray

    Surface exploration of the Moon and Asteroids can provide important information to scientists regarding the origins of the solar-system and life . Small robots and sensor modules can enable low-cost surface exploration. In the near future, they are the main machines providing these answers. Advanced in electronics, sensors and actuators enable ever smaller platforms, with compromising functionality. However similar advances haven't taken place for power supplies and thermal control system. The lunar south pole has temperatures in the range of -100 to -150 °C. Similarly, asteroid surfaces can encounter temperatures of -150 °C. Most electronics and batteries do not work below -40 °C. An effective thermal control system is critical towards making small robots and sensors module for extreme environments feasible. In this work, the feasibility of using thermochemical storage materials as a possible thermal control solution is analyzed for small robots and sensor modules for lunar and asteroid surface environments. The presented technology will focus on using resources that is readily generated as waste product aboard a spacecraft or is available off-world through In-Situ Resource Utilization (ISRU). In this work, a sensor module for extreme environment has been designed and prototyped. Our intention is to have a network of tens or hundreds of sensor modules that can communicate and interact with each other while also gathering science data. The design contains environmental sensors like temperature sensors and IMU (containing accelerometer, gyro and magnetometer) to gather data. The sensor module would nominally contain an electrical heater and insulation. The thermal heating effect provided by this active heater is compared with the proposed technology that utilizes thermochemical storage chemicals. Our results show that a thermochemical storage-based thermal control system is feasible for use in extreme temperatures. A performance increase of 80% is predicted for the sensor modules on the asteroid Eros using thermochemical based storage system. At laboratory level, a performance increase of 8 to 9 % is observed at ambient temperatures of -32°C and -40 °C.

  12. Enhanced electrocaloric analysis and energy-storage performance of lanthanum modified lead titanate ceramics for potential solid-state refrigeration applications.

    PubMed

    Zhang, Tian-Fu; Huang, Xian-Xiong; Tang, Xin-Gui; Jiang, Yan-Ping; Liu, Qiu-Xiang; Lu, Biao; Lu, Sheng-Guo

    2018-01-10

    The unique properties and great variety of relaxer ferroelectrics make them highly attractive in energy-storage and solid-state refrigeration technologies. In this work, lanthanum modified lead titanate ceramics are prepared and studied. The giant electrocaloric effect in lanthanum modified lead titanate ceramics is revealed for the first time. Large refrigeration efficiency (27.4) and high adiabatic temperature change (1.67 K) are achieved by indirect analysis. Direct measurements of electrocaloric effect show that reversible adiabatic temperature change is also about 1.67 K, which exceeds many electrocaloric effect values in current direct measured electrocaloric studies. Both theoretical calculated and direct measured electrocaloric effects are in good agreements in high temperatures. Temperature and electric field related energy storage properties are also analyzed, maximum energy-storage density and energy-storage efficiency are about 0.31 J/cm 3 and 91.2%, respectively.

  13. Development of media for dynamic latent heat storage for the low-temperature range. Part 1: Thermal analyses of selected salt hydrate systems

    NASA Technical Reports Server (NTRS)

    Kanwischer, H.; Tamme, R.

    1985-01-01

    Phase change temperatures and phase change enthalpies of seventeen salt hydrates, three double salts, and four eutectics were measured thermodynamically and the results reported herein. Good results were obtained, especially for congruently melting salt hydrates. Incongruently melting salt hydrates appear less suitable for heat storage applications. The influence of the second phase - water, acid and hydroxide - to the latent heat is described. From these results, basic values of the working temperatures and storage capabilities of various storage media compositions may be derived.

  14. On-farm storage of livestock vaccines may be a risk to vaccine efficacy: a study of the performance of on-farm refrigerators to maintain the correct storage temperature.

    PubMed

    Williams, Paul D; Paixão, Gustavo

    2018-04-19

    Livestock vaccines (LV) are often stored on-farm, in a refrigerator (fridge), prior to use and little is documented about the storage conditions during this period. As the quality of a vaccine can be impaired by storage at an incorrect temperature, the present study aimed to evaluate the on-farm performance of farm fridges to maintain the correct storage temperature. From January to August 2014, temperature data loggers were placed on selected farms fridges used to store LV (n = 20) in South-West England. Temperature recording data was available from 17 of the 20 farms. Fifty-nine percent of farm fridges had at least one temperature recording above 8 °C, 53% had at least one recording below 2 °C and 41% at or below 0 °C. Internal fridge temperatures attained 24 °C and dropped to - 12 °C as an absolute maximum and minimum respectively. Fridges tested spent an average of 16% of the total time recorded above 8 °C. Time of the year significantly influenced the percentage of time above 8 °C. External and internal temperatures were found to be positively correlated (p < 0.001). Statistical significant differences in internal and external temperatures were found between March and August. The majority of fridges in this study would have failed to keep any stored LV within the recommended storage temperature range. If LV are going to be stored on-farm prior to use, then urgent improvements in this part of the cold-chain are required in order to insure vaccine efficacy is not compromised.

  15. Graphic-output temperature data loggers for monitoring vaccine refrigeration: implications for pertussis.

    PubMed

    McColloster, Patrick; Vallbona, Carlos

    2011-01-01

    We conducted the first US study using graphic-output temperature data loggers in quantifying cold chain failure. Fifty-four vaccine refrigerators of a county outpatient health system were studied. Forty-eight percent maintained temperatures of 2°C to 8°C and 24% had protracted periods of temperatures less than 0°C. The correlation between the percentage of refrigerators with freezing temperatures and the pertussis rate for each health region was r = 0.76. The findings suggest that improper vaccine storage may have contributed to recent increases in pertussis rates.

  16. Fuel Temperature Fluctuations During Storage

    NASA Astrophysics Data System (ADS)

    Levitin, R. E.; Zemenkov, Yu D.

    2016-10-01

    When oil and petroleum products are stored, their temperature significantly impacts how their properties change. The paper covers the problem of determining temperature fluctuations of hydrocarbons during storage. It provides results of the authors’ investigations of the stored product temperature variations relative to the ambient temperature. Closeness and correlation coefficients between these values are given. Temperature variations equations for oil and petroleum products stored in tanks are deduced.

  17. Ex vivo spontaneous generation of 19-norandrostenedione and nandrolone detected in equine plasma and urine.

    PubMed

    Guan, Fuyu; Uboh, Cornelius E; Soma, Lawrence R; You, Youwen; Li, Xiaoqing; McDonnell, Sue

    2012-01-01

    19-Norandrostenedione (NAED) and nandrolone are anabolic-androgenic steroids (AASs). Nandrolone was regarded solely as a synthetic AAS until the 1980s when trace concentrations of apparently endogenous nandrolone were detected in urine samples obtained from intact male horses (stallions). Since then, its endogenous origin has been reported in boars and bulls; endogenous NAED and nandrolone have been identified in plasma and urine samples collected from stallions. More recently, however, it was suggested that NAED and nandrolone detected in urine samples from stallions are primarily artifacts due to the analytical procedure. The present study was undertaken to determine whether NAED and nandrolone detected in plasma and urine samples collected from stallions are truly endogenous or artifacts from sample processing. To answer this question, fresh plasma and urine samples from ≥8 stallions were analyzed for the two AASs, soon after collection, by liquid chromatography hyphenated to tandem mass spectrometry (LC-MS/MS). NAED and nandrolone were not detected in fresh plasma samples but detected in the same samples post storage. Concentrations of both AASs increased with storage time, and the increases were greater at a higher storage temperature (37°C versus 4°C, and ambient temperature versus 4°C). Although NAED was detected in some fresh stallion urine samples, its concentration (<407 pg/mL) was far lower (<0.4%) than that in the same samples post storage (at ambient temperature for 15 days). Nandrolone was not detected in most of fresh urine samples but detected in the same samples post storage. Based on these results, it is concluded that all NAED and nandrolone detected in stored plasma samples of stallions and most of them in the stored urine samples are not from endogenous origins but spontaneously generated during sample storage, most likely from spontaneous decarboxylation of androstenedione-19-oic acid and testosterone-19-oic acid. To our knowledge, it is the first time that all NAED and nandrolone detected in plasma of stallions and most of them detected in the urine have been shown to be spontaneously generated in vitro during sample storage. This finding would have significant implications with regard to the regulation of the two steroids in horse racing. Copyright © 2011 Elsevier Ltd. All rights reserved.

  18. Effects of Lower Drying-Storage Temperature on the Ductility of High-Burnup PWR Cladding

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Billone, M. C.; Burtseva, T. A.

    2016-08-30

    The purpose of this research effort is to determine the effects of canister and/or cask drying and storage on radial hydride precipitation in, and potential embrittlement of, high-burnup (HBU) pressurized water reactor (PWR) cladding alloys during cooling for a range of peak drying-storage temperatures (PCT) and hoop stresses. Extensive precipitation of radial hydrides could lower the failure hoop stresses and strains, relative to limits established for as-irradiated cladding from discharged fuel rods stored in pools, at temperatures below the ductile-to-brittle transition temperature (DBTT).

  19. Large electrostrictive effect in (Ba1-xGd2x/3)Zr0.3Ti0.7O3 relaxor towards moderate field actuator and energy storage applications

    NASA Astrophysics Data System (ADS)

    Ghosh, S. K.; Saha, Sujoy; Sinha, T. P.; Rout, S. K.

    2016-11-01

    The need of lead-free high performance ceramics with large electrostrictive effect, minimum hysteresis loss and energy storage ability at room temperature has become indispensable. At room temperature one of the key challenges in ceramic materials is to enhance the electrostrictive and energy storage properties simultaneously. In this regards, lead-free gadolinium modified barium zirconate titanate (Ba1-xGd2x/3)(Zr0.3Ti0.7)O3 (x = 0.02, 0.04, 0.06, 0.08, 0.10) ceramic was experimentally investigated to gain the competent electromechanical parameters near room temperature. Dielectric measurements exhibit a diffuse type of phase transition of relaxor phenomena and slim hysteresis loop with low remnant polarization and low hysteresis loss were observed. A moderate electric field of 30 kV/cm, recoverable energy and storage efficiency increases with Gd content. Strain-electric field hysteresis curves such as S-E, S-E2, and S-P2 profiles indicate improved electrostrictive characteristic of the ceramics. Results show that a maximum strain S ˜ 0.083% with large electrostrictive coefficient Q11 ˜ 0.054 m4/C2 and M11 ˜ 0.142 × 10-16 m2/V2 were obtained for x = 0.02 based BGdZT composition near relaxor-paraelectric phase boundary. The behavior of electrostrictive effect and energy storage efficiency suggested new possibilities of high precision lead-free ceramic actuator in a moderate field.

  20. Preservation of mithun (Bos frontalis) semen at refrigeration temperature.

    PubMed

    Karunakaran, M; Dhali, A; Mech, A; Khate, K; Rajkhowa, C; Mishra, D P

    2007-10-01

    The objective of the present study was to investigate the possibility of preserving mithun (Bos frontalis) spermatozoa at refrigeration temperature using tris-egg yolk diluent. Semen samples were collected from four adult mithun bulls through rectal massage method. Good quality semen samples (n=30) were preserved at 4 degrees C using tris-egg yolk diluent for 72 h. Progressive motility, live spermatozoa count and morphological abnormalities were evaluated every 12 h until 72 h of preservation. The colour, consistency and mass activity of fresh semen samples were found to be creamy white, medium and 3+ to 4+ (5+ scale), respectively. The average (mean+/-S.E.) volume (ml), pH and spermatozoa concentration (10(6) ml(-1)) of fresh semen samples were found to be 0.6+/-0.01, 6.8+/-0.03 and 425+/-48, respectively. Progressive motility and live spermatozoa count were found to be less than 30% (P<0.01) after 48 h of storage. Head (P<0.05), midpiece (P<0.05), tail (P<0.01) and total (P<0.01) abnormalities were found to be increased significantly over the time of storage. It was observed that progressive motility and live spermatozoa count remained above 30% and 40%, respectively, until 36 h of storage. Simultaneously the percentage of morphologically abnormal spermatozoa was found to be significantly low until 36 h of storage. The results indicate that it is possible to preserve mithun spermatozoa at refrigeration temperature in tris-egg yolk diluent, which can be further used for artificial insemination within 36 h of storage.

  1. Mechanical properties of three layer glass fibre reinforced unsaturated polyester filled with P84 Polyimide

    NASA Astrophysics Data System (ADS)

    Ibrahim, Nik Noor Idayu Nik; Mamauod, Siti Nur Liyana; Romli, Ahmad Zafir

    2017-12-01

    The glass fibre reinforced orthophthalic unsaturated polyester composite was widely used in the pipeline industry as a replacement to the corroded steel pipes. A filler which possesses high mechanical performance at high temperature; P84 Polyimide used as the particulate reinforcement in the unsaturated polyester matrix system to increase the mechanical performance of the glass fibre reinforced unsaturated polyester. The glass fibre composite laminates were prepared through a hand lay-up technique and fabricated into three layer laminate. Prior to be used as the matrix system in the lamination process, the unsaturated polyester resin was mixed with masterbatch P84 Polyimide at three loadings amount of 1, 3, and 5 wt%. The addition of P84 Polyimide at 1, 3, and 5 wt% increased the tensile properties and flexural properties especially at 1 wt% filler loading. As the filler loading increased, the tensile properties and flexural properties showed decreasing pattern. In the dynamic mechanical analysis, the values of storage modulus were taken at two points; 50 °C and 150 °C which were the storage modulus before and after the glass transition temperature. All storage modulus showed fluctuation trend for both before and after Tg. However, the storage modulus of the filled composite laminates after Tg showed higher values than unfilled composite laminates at all filler loading. Since the P84 Polyimide possesses high thermal stability, the presence of P84 Polyimide inside the composite system had assisted in delaying the Tg. In terms of the filler dispersion, the Cole-Cole plot showed an imperfect semi-circular shape which indicated good filler dispersion.

  2. Salmonella survival during thermal dehydration of fresh garlic and storage of dehydrated garlic products.

    PubMed

    Zhang, Hongmei; Qi, Yan; Wang, Lei; Zhang, Shaokang; Deng, Xiangyu

    2017-12-18

    Salmonella survival was characterized and modeled during thermal dehydration of fresh garlic and storage of dehydrated garlic products. In our experiments that simulated commercial dehydration processing at 80±5°C, moderate level of Salmonella contamination (4-5logCFU/g) on fresh garlic was reduced below the enumeration limit (1.7logCFU/g) after 4.5h of dehydration and not detectable by culture enrichment after 7h. With high level of contamination (7-8logCFU/g), the Salmonella population persisted at 3.6logCFU/g after 8h of processing. By increasing the dehydration temperature to 90±5°C, the moderate and high levels of initial Salmonella load on fresh garlic dropped below the enumeration limit after 1.5 and 3.75h of processing and became undetectable by culture enrichment after 2.5 and 6h, respectively. During the storage of dried garlic products, Salmonella was not able to grow under all tested combinations of temperature (25 and 35°C) and water activity (0.56-0.98) levels, suggesting active inhibition. Storage temperature played a primary role in determining Salmonella survival on dehydrated garlic flakes. Under a typical storage condition at 25°C and ambient relative humidity, Salmonella could persist over months with the population gradually declining (4.3 log reduction over 88days). Granular size of dehydrated garlic had an impact on Salmonella survival, with better survival of the pathogen observed in bigger granules. At the early stage of dehydrated garlic storage (until 7days), rising water activity appeared to initially promote but then inhibited Salmonella survival, resulting in a water activity threshold at 0.73 where Salmonella displayed strongest persistence. However, this phenomenon was less apparent during extended storage (after 14days). Copyright © 2017 Elsevier B.V. All rights reserved.

  3. Influence of storage and heating on protein glycation levels of processed lactose-free and regular bovine milk products.

    PubMed

    Milkovska-Stamenova, Sanja; Hoffmann, Ralf

    2017-04-15

    Thermal treatment preserves the microbiological safety of milk, but also induces Maillard reactions modifying for example proteins. The purpose of this study was evaluating the influence of consumer behaviors (storage and heating) on protein glycation degrees in bovine milk products. Lactosylation and hexosylation sites were identified in ultra-high temperature (UHT), lactose-free pasteurized, and lactose-free UHT milk (ULF) and infant formula (IF) using tandem mass spectrometry (electron transfer dissociation). Overall, 303 lactosylated and 199 hexosylated peptides were identified corresponding to 170 lactosylation (31 proteins) and 117 hexosylation sites (25 proteins). In quantitative terms, storage increased lactosylation up to fourfold in UHT and IF and hexosylation up to elevenfold in ULF and threefold in IF. These levels increased additionally twofold when the stored samples were heated (40°C). In conclusion, storage and heating appear to influence protein glycation levels in milk at similar or even higher degrees than industrial processing. Copyright © 2016 Elsevier Ltd. All rights reserved.

  4. Experimental investigation of a packed bed thermal energy storage system

    NASA Astrophysics Data System (ADS)

    Cascetta, Mario; Cau, Giorgio; Puddu, Pierpaolo; Serra, Fabio

    2015-11-01

    In this work experimental investigations on a thermal energy storage system with a solid material as storage media and air as heat transfer fluid will be presented. The experimental test rig, installed at the DIMCM of the University of Cagliari, consists of a carbon steel tank filled with freely poured alumina beads that allows investigations of heat transfer phenomena in packed beds. The aim of this work is to show the influence of the operating conditions and physical parameters on thermocline formation and, in particular, the thermal behaviour of the thermal energy storage for repeated charging and discharging cycles. Better charging efficiency is obtained for lower values of mass flow rate and maximum air temperature and for increasing aspect ratio. A decreasing influence of the metal wall with continuous operation is also highlighted. In conclusion, the analysis focuses on the thermal hysteresis phenomenon, which causes degradation of the thermocline and the reduction of the energy that can be stored by the accumulator as the repeated number of cycles increases.

  5. Impact of canning and storage on apricot carotenoids and polyphenols.

    PubMed

    Le Bourvellec, Carine; Gouble, Barbara; Bureau, Sylvie; Reling, Patrice; Bott, Romain; Ribas-Agusti, Albert; Audergon, Jean-Marc; Renard, Catherine M G C

    2018-02-01

    Apricot polyphenols and carotenoids were monitored after industrial and domestic cooking, and after 2months of storage for industrial processing. The main apricot polyphenols were flavan-3-ols, flavan-3-ol monomers and oligomers, with an average degree of polymerization between 4.7 and 10.7 and caffeoylquinic acids. Flavonols and anthocyanins were minor phenolic compounds. Upon processing procyanidins were retained in apricot tissue. Hydroxycinnamic acids, flavan-3-ol monomers, flavonols and anthocyanins leached in the syrup. Flavonol concentrations on per-can basis were significantly increased after processing. Industrial processing effects were higher than domestic cooking probably due to higher temperature and longer duration. After 2months of storage, among polyphenols only hydroxycinnamic acids, flavan-3-ol monomers and anthocyanins were reduced. Whichever the processing method, no significant reductions of total carotenoids were observed after processing. The cis-β-carotene isomer was significantly increased after processing but with a lower extent in domestic cooking. Significant decreased in total carotenoid compounds occurred during storage. Copyright © 2017 Elsevier Ltd. All rights reserved.

  6. High Temperature Latent Heat Thermal Energy Storage to Augment Solar Thermal Propulsion for Microsatellites

    DTIC Science & Technology

    2014-03-01

    Charts 3. DATES COVERED (From - To) Mar 2014- May 2014 4. TITLE AND SUBTITLE 5a. CONTRACT NUMBER In-House High Temperature Latent Heat Thermal ...Energy Storage to Augment Solar Thermal Propulsion for Microsats 5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) 5d. PROJECT NUMBER...High Temperature Latent Heat Thermal Energy Storage to Augment Solar Thermal Propulsion for Microsatellites Presentation Subtitle Matthew R. Gilpin

  7. Improved running performance in hot humid conditions following whole body precooling.

    PubMed

    Booth, J; Marino, F; Ward, J J

    1997-07-01

    On two separate occasions, eight subjects controlled speed to run the greatest distance possible in 30 min in a hot, humid environment (ambient temperature 32 degrees C, relative humidity 60%). For the experimental test (precooling), exercise was preceeded by cold-water immersion. Precooling increased the distance run by 304 +/- 166 m (P < 0.05). Precooling decreased the pre-exercise rectal and mean skin temperature by 0.7 degrees C and 5.9 degrees C, respectively (P < 0.05). Rectal and mean skin temperature were decreased up to 20 and 25 min during exercise, respectively (P < 0.05). Mean body temperature decreased from 36.5 +/- 0.1 degrees C to 33.8 +/- 0.2 degrees C following precooling (P < 0.05) and remained lower throughout exercise (P < 0.01) and at the end of exercise (by 0.8 degrees C; P < 0.05). The rate of heat storage at the end of exercise increased from 113 +/- 45 to 249 +/- 55 W.m-2 (P < 0.005). Precooling lowered the heart rate at rest (13%), 5 (9%), and 10 min (10%) exercise (P < 0.05) and increased the end of exercise blood lactate from 4.9 +/- 0.5 to 7.4 +/- 0.9 mmol.L-1 (P < 0.01). The VO2 at 10 and 20 min of exercise and total body sweating are not different between tests. In conclusion, water immersion precooling increased exercise endurance in hot, humid conditions with an enhanced rate of heat storage and decreased thermoregulatory strain.

  8. Effect of temperature-driven phase transition on energy-storage and -release properties of Pb0.97La0.02[Zr0.55Sn0.30Ti0.15]O3 ceramics

    NASA Astrophysics Data System (ADS)

    Xu, Ran; Tian, Jingjing; Zhu, Qingshan; Feng, Yujun; Wei, Xiaoyong; Xu, Zhuo

    2017-07-01

    Temperature-driven phase transition of Pb0.97La0.02[Zr0.55Sn0.30Ti0.15]O3 ceramics was studied, and the consecutive ferroelectric-antiferroelectric-paraelectric (FE-AFE-PE) switching was confirmed. The materials have better dielectric tunability (-82% to 50%) in the AFE state than in the FE state. Also, the phase transition influences the energy-storage and -release performance significantly. A sharp increase in releasable energy density and efficiency was observed due to the temperature-driven FE-AFE transition. Highest releasable energy density, current density, and peak power density were achieved at 130 °C, which was attributed to the highest backward transition field. The stored charge was released completely in AFE and PE states in the microseconds scale, while only a small part of it was released in the FE state. The above results indicate the huge impact of temperature-driven phase transition on dielectrics' performance, which is significant when developing AFE materials working in a wide temperature range.

  9. Effect of total solids content and temperature on the rheological behaviour of reconstituted whole milk concentrates.

    PubMed

    Trinh, Binh; Trinh, Khanh Tuoc; Haisman, Derek

    2007-02-01

    This work investigated the combined effect of solids content, heating and storage temperatures on the rheological behaviour of reconstituted whole milk concentrates. The powder was reconstituted at 35 degrees C in a custom built recombination rig to various total solids content (TS) from 10-48% TS. The concentrates were then heated to 45-85 degrees C and stored at the heating temperature. The rheological behaviour shifter from Newtonian behaviour (below 30% TS) to power law (below 40% TS), with the yield stress observed from 40% TS upwards and time-dependent behaviour was noticed above 44% TS. Higher heating temperatures tend to promote non-Newtonian behaviour at lower solids content. The viscosity-solid content curve showed an exponential relationship, while the viscosity-temperature curve exhibited a minimum at 65-75 degrees C above 46% TS. During age thickening, the yield stress and the consistency coefficient increased, while the flow behaviour index decreased with storage time. This indicated that the milk concentrates deviated away from Newtonian behaviour during age thickening.

  10. Preservation of Biospecimens at Ambient Temperature: Special Focus on Nucleic Acids and Opportunities for the Biobanking Community.

    PubMed

    Muller, Rolf; Betsou, Fay; Barnes, Michael G; Harding, Keith; Bonnet, Jacques; Kofanova, Olga; Crowe, John H

    2016-04-01

    Several approaches to the preservation of biological materials at ambient temperature and the relative impact on sample stability and degradation are reviewed, with a focus on nucleic acids. This appraisal is undertaken within the framework of biobank risk, quality management systems, and accreditation, with a view to assessing how best to apply ambient temperature sample storage to ensure stability, reduce costs, improve handling logistics, and increase the efficiency of biobank procedures.

  11. Cesium Sorption/Desorption Experiments with IONSIV(R) IE-911 in Radioactive Waste

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Walker, D.D.

    2001-02-13

    This report describes cesium desorption from IONSIV IE-911 during ambient temperature storage and following temperature increases to 35 and 55 degrees C. This report also describes cesium sorption following return to ambient temperature. The IONSIV IE-911 used in these tests was loaded with cesium from Tank 44F radioactive waste in an ion exchange column test in 1999. Cesium desorbed and resorbed in the presence of Tank 44F waste and simulated waste solutions.

  12. Thermal gelation profile changes in reconstituted actomyosin due to storage under a high salt concentration and low temperature.

    PubMed

    Tanji, H; Ikeuchi, Y; Yoshizawa, M; Suzuki, A

    1997-05-01

    Changes in the heat-induced gelation properties of reconstituted rabbit skeletal actomyosin stored under a high salt concentration at pH 6.0 and 0 degree C were investigated at different weight ratios of actin to myosin by using dynamic rheological and biochemical measurements. The addition of actin resulted in a pronounced peak maximum at about 50 degrees C and an accompanying temporary reduction in the range at about 50 degrees C to 60 degrees C. The more the initial actin concentration was increased, the greater was the area of the peak/shoulder. However, this area was markedly diminished with increasing storage time. As a result, the dynamic rheological pattern was transformed from an actomyosin type into a myosin type. The relationship between the G' value at 80 degrees C and the actin/myosin weight ratio was curvilinear, with a peak at the ratio of 0.05, immediately after storage was started. This profile changed during storage, depending on the extent to denaturation of actin and myosin in the reconstituted actomyosin (RAM). The G' value of actomyosin in 0.5 M KCl with a small actin/myosin ratio of 0.05 decreased to one-half of its initial value after 7 days of storage, whereas the G' value with a large actin/myosin ratio of 0.225 increased by about 1.6 times. In 1.5 M KCl, all the G' values declined to the level with myosin alone after 7 days of storage. The time-course plots of the remaining actin concentration in RAM at different weight ratios of actin to myosin after being treated with 0.5 M or 1.5 M KCl showed a decrease in the actin content with increasing storage time, and an increase in the KCl concentration to 1.5 M KCl promoted the denaturation of actin in RAM faster than with 0.5 M KCl. The surface hydrophobicity of each RAM sample progressively increased with increasing storage time, while little significant increase in the sulfhydryl (SH) content during storage was observed. It is concluded that changes in the heat-induced gelation properties of actomyosin during storage are largely attributable to the denaturation of actin rather than to the denaturation of myosin or to quantitative changes in the SH content and hydrophobicity.

  13. Analysis Total Plate Counte (TPC) On Fresh Steak Tuna Applications Edible Coating Caulerpa sp During Stored at Chilling Temperature

    NASA Astrophysics Data System (ADS)

    Nelce Mailoa, Meigy; Marthina Tapotubun, Alfonsina; Matrutty, Theodora E. A. A.

    2017-10-01

    A study has been conducted to determine the use of Caulerpa sp. Edible coatings on fresh steaks tuna against the presence of microbes during storage at chilling temperatures. In this research, two applied method of edible coating is used, that is dipping and immersion method, with chilling temperature (5°C), storage time (0, 3, 6, 9 days). Each treatment is compared to control (without an edible coating). The results showed that the application of edible coating Caulerpa sp in fresh steaks tuna with soaking and dipping method showed total bacteria increase during storage, but still fulfill the microbiological quality of fresh fish that is maximum total plate 5.0 × 105 cfu/g. Total microbes in fresh steaks tuna were soaked with immersion methods and stored up to 9 days: 2.3 × 105 cfu/g while total microbial on fresh steaks tuna were dipped by dipping method and stored up to 9 days ie 3.4 × 105 cfu/g. So it can be concluded that application method of edible coating Caulerpa sp on fresh steaks tuna soaked better than the method of dipping.

  14. Doped calcium manganites for advanced high-temperature thermochemical energy storage

    DOE PAGES

    Babiniec, Sean M.; Coker, Eric N.; Miller, James E.; ...

    2015-12-16

    Developing efficient thermal storage for concentrating solar power plants is essential to reducing the cost of generated electricity, extending or shifting the hours of operation, and facilitating renewable penetration into the grid. Perovskite materials of the CaB xMn 1-xO 3-δ family, where B = Al or Ti, promise improvements in cost and energy storage density over other perovskites currently under investigation. Thermogravimetric analysis of the thermal reduction and reoxidation of these materials was used to extract equilibrium thermodynamic parameters. Lastly, the results demonstrate that these novel thermochemical energy storage media display the highest reaction enthalpy capacity for perovskites reported tomore » date, with a reaction enthalpy of 390 kJ/kg, a 56% increase over previously reported compositions.« less

  15. Effects of Preheating and Storage Temperatures on Aroma Profile and Physical Properties of Citrus-Oil Emulsions.

    PubMed

    Yang, Ying; Zhao, Chengying; Tian, Guifang; Lu, Chang; Zhao, Shaojie; Bao, Yuming; McClements, David Julian; Xiao, Hang; Zheng, Jinkai

    2017-09-06

    Citrus oils are used as good carrier oil for emulsion fabrication due to their special flavor and various health-promoting functions. In this study, the effects of preheating temperature (30, 40, 50, 60, and 70 °C) and storage temperature (4, 25, and 37 °C) on aroma profiles and physical properties of three citrus-oil (i.e., mandarin, sweet orange, and bergamot oils) emulsions were systematically investigated for the first time. The results demonstrated the significant impact of temperature on aroma profile and physical properties. The abundance of d-limonene was found to be the main factor determining the aroma of the three citrus-oil emulsions at different preheating and storage temperatures, while β-linalool and linalyl acetate were important for the aroma of bergamot oil emulsion. Preheating temperature showed a profound impact on the aroma of citrus-oil emulsions, and the aroma of different citrus oil emulsions showed different sensitivity to preheating temperature. Storage temperature was also able to alter the properties of citrus oil emulsions. The higher was the storage temperature, the more alteration of aroma and more instability of the emulsions there was, which could be attributed to the alteration of the oil components and the properties of emulsions. Among all three emulsions, bergamot-oil emulsion was the most stable and exhibited the most potent ability to preserve the aroma against high temperature. Our results would facilitate the application of citrus-oil emulsions in functional foods and beverages.

  16. Nonenzymatic Browning and Protein Aggregation in Royal Jelly during Room-Temperature Storage.

    PubMed

    Qiao, Jiangtao; Wang, Xueyu; Liu, Liqiang; Zhang, Hongcheng

    2018-02-28

    Royal jelly possesses numerous functional properties. Improper storage usually causes bioactivity loss, especially queen differentiation activity. To determine changes in royal jelly, we investigated nonenzymatic browning and protein changes in royal jelly during room-temperature storage from 1 to 6 months. Our results indicate that royal jelly experiences nonenzymatic browning and protein aggregation. The products of nonenzymatic browning dramatically increased, especially N ε -carboxymethyl lysine (CML) with growth of approximately 7-fold. We speculate that CML may be recognized as a freshness marker for royal jelly. Our results also demonstrate that the major royal jelly protein 1 (MRJP1) monomer gradually aggregated with MRJP1 oligomers into new oligomers of about 440 and 700 kDa. This suggests that the reduction of MRJP1 monomer may be attributable to aggregation. We provide the novel explanation that the differentiation loss of royal jelly may be due to the aggregation of MRJP1 limiting the honeybees' ability to digest and absorb royal jelly.

  17. A Possible Freshness Marker for Royal Jelly: Formation of 5-Hydroxymethyl-2-furaldehyde as a Function of Storage Temperature and Time.

    PubMed

    Ciulu, Marco; Floris, Ignazio; Nurchi, Valeria M; Panzanelli, Angelo; Pilo, Maria I; Spano, Nadia; Sanna, Gavino

    2015-04-29

    In this article we present a study of the variability of the concentration of 5-hydroxymethyl-2-furaldehyde (HMF) in natural royal jelly (RJ) as a function of its storage temperature (-18, 4, and 25 °C) and time (up to 9 months after harvesting). For this work HMF is evaluated using an RP-HPLC method we previously assessed. While all RJ samples stored at 4 and -18 °C always showed levels of HMF under the limit of detection (0.13 mg kg(-1)), samples kept at 25 °C showed an exponential increase in the concentration of HMF as a function of the storage time. This behavior and a number of desirable features of the analytical method used (ease of use in routine laboratories, availability of a complete validation protocol specifically developed for RJ, based on consolidated chemical knowledge) allow us to hypothesize the use of HMF as a possible, reliable freshness marker for RJ.

  18. DOE Office of Scientific and Technical Information (OSTI.GOV)

    Zhou, Hongcai J

    In the past decades, there has been an escalation of interest in the study of MOFs due to their fascinating structures and intriguing application potentials. Their exceptionally high surface areas, uniform yet tunable pore sizes, and well-defined adsorbate-MOF interaction sites make them suitable for hydrogen storage. Various strategies to increase the hydrogen capacity of MOFs, such as constructing pore sizes comparable to hydrogen molecules, increasing surface area and pore volume, utilizing catenation, and introducing coordinatively unsaturated metal centers (UMCs) have been widely explored to increase the hydrogen uptake of the MOFs. MOFs with hydrogen uptake approaching the DOE gravimetric storagemore » goal under reasonable pressure but cryo- temperature (typically 77 K) were achieved. However, the weak interaction between hydrogen molecules and MOFs has been the major hurdle limiting the hydrogen uptake of MOFs at ambient temperature. Along the road, we have realized both high surface area and strong interaction between framework and hydrogen are equally essential for porous materials to be practically applicable in Hydrogen storage. Increasing the isosteric heats of adsorption for hydrogen through the introduction of active centers into the framework could have great potential on rendering the framework with strong interaction toward hydrogen. Approaches on increasing the surface areas and improving hydrogen affinity by optimizing size and structure of the pores and the alignment of active centers around the pores in frameworks have been pursued, for example: (a) the introduction of coordinatively UMC (represents a metal center missing multiple ligands) with potential capability of multiple dihydrogen-binding (Kubas type, non-dissociative) per UMC, (b) the design and synthesis of proton-rich MOFs in which a + H3 binds dihydrogen just like a metal ion does, and (c) the preparation of MOFs and PPNs with well aligned internal electric fields. We believe the accomplishments of this DOE supported research will greatly benefit the future pursuit of hydrogen storage materials. The ultimate goal to increase the gravimetric and volumetric hydrogen storage capacity to meet DOE targets for Light-Duty Vehicles is achievable.« less

  19. Effect of various refrigeration temperatures on quality of shell eggs.

    PubMed

    Shin, Daekeun; Narciso-Gaytán, Carlos; Regenstein, Joe M; Sánchez-Plata, Marcos X

    2012-05-01

    The objective of this study was to evaluate the effects of low storage temperatures on shell egg quality. Approximately 2100 shell eggs were collected and stored at - 1.1, 0.6, 2.2, 3.9, 5.6 and 7.2 °C for up to 4 weeks. Eighteen eggs at each storage temperature were evaluated after 0, 2, 7, 14, 21 and 28 days of storage. Haugh units (HU), yolk index (YI), albumen pH (pHA), yolk pH (pHY) and angel food cake density (CD) were measured. Shell egg quality tended to be preserved better at below 2.2 °C, as high HU and YI values relative to eggs stored at 7.2 °C were determined on day 28. However, storage at - 1.1 °C tended to cause the opposite effect, especially highly declined HU values over time. Significantly different HU values of shell eggs were measured after 14 days of storage, with eggs stored at 0.6 and 2.2 °C having the highest HU values, 80.42 and 77.97 respectively. A lower temperature limit for shell egg storage could be established between 0.6 and 2.2 °C, as both temperatures showed the highest HU values, 77.88 and 77.60 respectively, after 28 days of storage. Copyright © 2011 Society of Chemical Industry.

  20. Changes in the enzymatic activity of soil samples upon their storage

    NASA Astrophysics Data System (ADS)

    Dadenko, E. V.; Kazeev, K. Sh.; Kolesnikov, S. I.; Val'Kov, V. F.

    2009-12-01

    The influence of the duration and conditions of storage of soil samples on the activity of soil enzymes (catalase, β-fructofuranosidase, and dehydrogenase) was studied for the main soils of southern Russia (different subtypes of chernozems, chestnut soils, brown forest soils, gray forest soils, solonetzes, and solonchaks). The following soil storage conditions were tested: (1) the air-dry state at room temperature, (2) the airdry state at a low positive (in a refrigerator, +4°C) temperature, (3) naturally moist samples at a low positive temperature, and (4) naturally moist samples at a negative (in a freezer, -5°C) temperature. It was found that the sample storing caused significant changes in the enzymatic activities, which depended on the soil type, the land use, the type of enzyme, and the duration and conditions of the sample storage. In the course of the storage, the changes in the enzymatic activity had a nonlinear character. The maximum changes were observed in the initial period (up to 12 weeks). Then, a very gradual decrease in the activity of the studied enzymes was observed. Upon the long-term (>12 weeks) storage under the different conditions, the difference in the activities of the soil enzymes became less pronounced. The storage of soil samples in the air-dried state at room temperature can be recommended for mass investigations.

  1. Impact of temperature and time storage on the microbial detection of oral samples by Checkerboard DNA-DNA hybridization method.

    PubMed

    do Nascimento, Cássio; dos Santos, Janine Navarro; Pedrazzi, Vinícius; Pita, Murillo Sucena; Monesi, Nadia; Ribeiro, Ricardo Faria; de Albuquerque, Rubens Ferreira

    2014-01-01

    Molecular diagnosis methods have been largely used in epidemiological or clinical studies to detect and quantify microbial species that may colonize the oral cavity in healthy or disease. The preservation of genetic material from samples remains the major challenge to ensure the feasibility of these methodologies. Long-term storage may compromise the final result. The aim of this study was to evaluate the effect of temperature and time storage on the microbial detection of oral samples by Checkerboard DNA-DNA hybridization. Saliva and supragingival biofilm were taken from 10 healthy subjects, aliquoted (n=364) and processed according to proposed protocols: immediate processing and processed after 2 or 4 weeks, and 6 or 12 months of storage at 4°C, -20°C and -80°C. Either total or individual microbial counts were recorded in lower values for samples processed after 12 months of storage, irrespective of temperatures tested. Samples stored up to 6 months at cold temperatures showed similar counts to those immediately processed. The microbial incidence was also significantly reduced in samples stored during 12 months in all temperatures. Temperature and time of oral samples storage have relevant impact in the detection and quantification of bacterial and fungal species by Checkerboard DNA-DNA hybridization method. Samples should be processed immediately after collection or up to 6 months if conserved at cold temperatures to avoid false-negative results. Copyright © 2013 Elsevier Ltd. All rights reserved.

  2. Concentrating Solar Power Projects - Crescent Dunes Solar Energy Project |

    Science.gov Websites

    : None Thermal Storage Storage Type: 2-tank direct Storage Capacity: 10 hours Thermal Storage Description : Thermal energy storage achieved by raising salt temperature from 550 to 1050 F. Thermal storage efficiency

  3. Effect of sample storage temperature and buffer formulation on faecal immunochemical test haemoglobin measurements.

    PubMed

    Symonds, Erin L; Cole, Stephen R; Bastin, Dawn; Fraser, Robert Jl; Young, Graeme P

    2017-12-01

    Objectives Faecal immunochemical test accuracy may be adversely affected when samples are exposed to high temperatures. This study evaluated the effect of two sample collection buffer formulations (OC-Sensor, Eiken) and storage temperatures on faecal haemoglobin readings. Methods Faecal immunochemical test samples returned in a screening programme and with ≥10 µg Hb/g faeces in either the original or new formulation haemoglobin stabilizing buffer were stored in the freezer, refrigerator, or at room temperature (22℃-24℃), and reanalysed after 1-14 days. Samples in the new buffer were also reanalysed after storage at 35℃ and 50℃. Results were expressed as percentage of the initial concentration, and the number of days that levels were maintained to at least 80% was calculated. Results Haemoglobin concentrations were maintained above 80% of their initial concentration with both freezer and refrigerator storage, regardless of buffer formulation or storage duration. Stability at room temperature was significantly better in the new buffer, with haemoglobin remaining above 80% for 20 days compared with six days in the original buffer. Storage at 35℃ or 50℃ in the new buffer maintained haemoglobin above 80% for eight and two days, respectively. Conclusion The new formulation buffer has enhanced haemoglobin stabilizing properties when samples are exposed to temperatures greater than 22℃.

  4. Bacterial cellulose composites loaded with SiO2 nanoparticles: Dynamic-mechanical and thermal properties.

    PubMed

    Sheykhnazari, Somayeh; Tabarsa, Taghi; Ashori, Alireza; Ghanbari, Abbas

    2016-12-01

    The aim of this paper was to prepare composites of bacterial cellulose (BC) filled with silica (SiO 2 ) nanoparticles to evaluate the influence of the SiO 2 contents (3, 5 and 7wt%) on the thermo-mechanical properties of the composites. BC hydro-gel was immersed in an aqueous solution of silanol derived from tetraethoxysilane (TEOS), the silanol was then converted into SiO 2 in the BC matrix by pressing at 120°C and 2MPa. The BC/SiO 2 translucent sheets were examined by dynamic-mechanical analysis (DMA), thermo gravimetric analysis (TGA), and scanning electron microscopy (SEM). The temperature dependence of the storage modulus, loss modulus and tan delta was determined by DMA. In general, the results revealed that the increment of storage modulus and thermal stability increased concomitantly with the augmentation of SiO 2 content. Therefore, it could be concluded that the mechanical properties of the composites were improved by using high amounts of nano silica. This would be a high aspect ratio of BC capable of connecting the BC matrix and SiO 2 , thereby enhancing a large contact surface and resulting in excellent coherence. A decrease of the storage modulus was consistent with increasing temperature, resulting from softening of the composites. The storage modulus of the composites increased in the order: BC/S7>BC/S5>BC/S3, while the loss modulus and tan delta decreased. On the other hand, the thermal stabilities of all BC/SiO 2 composites were remarkably enhanced as compared to the pristine BC. TGA curves showed that the temperature of decomposition of the pure BC gradually shifted from about 260°C to about 370°C as silica content increased. SEM observations illustrated that the nano-scale SiO 2 was embedded between the voids and nano-fibrils of the BC matrix. Overall, the results indicated that the successful synthesis and superior properties of BC/SiO 2 advocate its effectiveness for various applications. Copyright © 2016 Elsevier B.V. All rights reserved.

  5. Calibrated photostimulated luminescence is an effective approach to identify irradiated orange during storage

    NASA Astrophysics Data System (ADS)

    Jo, Yunhee; Sanyal, Bhaskar; Chung, Namhyeok; Lee, Hyun-Gyu; Park, Yunji; Park, Hae-Jun; Kwon, Joong-Ho

    2015-06-01

    Photostimulated luminescence (PSL) has been employed as a fast screening method for various irradiated foods. In this study the potential use of PSL was evaluated to identify oranges irradiated with gamma ray, electron beam and X-ray (0-2 kGy) and stored under different conditions for 6 weeks. The effects of light conditions (natural light, artificial light, and dark) and storage temperatures (4 and 20 °C) on PSL photon counts (PCs) during post-irradiation periods were studied. Non-irradiated samples always showed negative values of PCs, while irradiated oranges exhibited intermediate results after first PSL measurements. However, the irradiated samples had much higher PCs. The PCs of all the samples declined as the storage time increased. Calibrated second PSL measurements showed PSL ratio <10 for the irradiated samples after 3 weeks of irradiation confirming their irradiation status in all the storage conditions. Calibrated PSL and sample storage in dark at 4 °C were found out to be most suitable approaches to identify irradiated oranges during storage.

  6. Influence of putrescine and carnauba wax on functional and sensory quality of pomegranate (Punica granatum L.) fruits during storage.

    PubMed

    Barman, Kalyan; Asrey, Ram; Pal, R K; Kaur, Charanjit; Jha, S K

    2014-01-01

    Functional properties (anthocyanins, antioxidant, ascorbic acid and tannin) and sensory score were determined in pomegranate fruits at two storage temperatures (3 and 5 °C) after treatment with 2 mM putrescine and 1 : 10 carnauba wax (carnauba wax : water). The treatments (putrescine and carnauba wax) were given by immersion method followed by storage up to 60 days. Both treatments retained significantly higher anthocyanins, antioxidant, ascorbic acid, tannin and sensory qualities as compared with control fruits under both the storage conditions. Combined application of putrescine + carnauba wax showed better response in retaining functional properties than putrescine treated or nontreated fruits. The impacts of putrescine and carnauba wax treatments were found more pronounced after 30 days at 3-5 °C storage temperature in retaining functional and sensory qualities. After 60 days of storage, putrescine + carnauba wax retained about 25% higher antioxidant activity both at 3 and 5 °C storage temperatures.

  7. Unsteady Heat Transfer Behavior of Reinforced Concrete Wall of Cold Storage

    NASA Astrophysics Data System (ADS)

    Nomura, Tomohiro; Murakami, Yuji; Uchikawa, Motoyuki

    The authors had already clarified that the heat transfer behaviors between internal and external insulated reinforced concrete wall of cold storage are different each others when inside and outside temperature of wall is flactuating. From that conclusion, we must consider the application method of wall insulation of cold storages in actual design. The theme of the paper is to get the analyzing method and unsteady heat transfer characteristics of concrete walls of cold storage during daily variation of outside temperature of walls, and to give the basis for efficient design and cost optimization of insulate wall of cold storage. The difference of unsteady heat transfer characteristics between internal and external insulate wall, when outside temperature of the wall follewed daily varation, was clarified in experiment and in situ measurement of practical cold storage. The analyzing method with two dimentional unsteady FEM was introduced. Using this method, it is possible to obtain the time variation of heat flux, which is important basic factor for practical design of cold storage, through the wall.

  8. INFLUENCE OF INTRAMUSCULAR FAT LEVEL ON ORGANOLEPTIC, PHYSICAL, AND CHEMICAL CHARACTERISTICS OF IRRADIATED PORK MUSCLE

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Whitehair, L.A.

    1962-01-01

    A study was conducted to evaluate the influence of marbling on certain organoleptic, physical, and chemical characteristics of precooked and irradiated pork loin muscle (longissimus dorsi). The study consisted of two separate phases. Loins utilized in Phase I were selected by visual appraisal and categorized into three distinct marbling level scores. A high temperature, short time blanching treatment was used for proteolytic enzyme inactivation. Phase II loins were selected and classified into three marbling levels by both visual appraisal amd ether extraction analysis of total fat. A low temperature, long time heat treatment was used for enzyme inactivation. Samples weremore » packed under vacuum in rigid containers and irradiated at a dosage level of 4.5 megarads gamma radiation. Non-irradiated samples were stored at -65 deg F. Irradiated and control samples were evaluated at periodic intervals by a panel. Physical and chemical analyses were made initially on samples representing each treatment and subsequently at the termination of each storage period. Organoleptic results indicated that degree of marbling did not influence preference ratings of plain radiosterilized longissimus dorsi muscle (pork). However, irradiated longissimus dorsi (pork) sandwich items with lower marbling scores were consistently preferred over highly marbled, irradiated sandwich items. Non-irradiated longissimus dorsi samples were preferred to irradiated longissimus dorsi samples in all tests. The short term-high temperature method of blanching used in Phase I resulted in products of slightly superior quality to those of Phase II, which possessed softer, slightly drier texture characteristics. The practical storage life of irradiated samples under the conditions was approximately 150 days. Hunter color values were increased by radiation treatment. Irradiated longissimus dorsi samples developed a characteristic pink-red color. Mechanical tenderness values in both irradiated and non-irradiated samples were lowered significantly by higher levels of marbling. Expressible moisture values of irradiated samples were lower than those of control samples. The values increased with advancing storage time. Iodine numbers of lower marbling scores (both irradiated and non-irradiated samplcs) exceeded those of highly marbled samples. T.B.A. number values were lower in irradiated samples of Phase I. The values were increased with respect to increased levels of marbling in Phase II. Values increased steadily with advancing storage time in both phases. pH values were elevated by irradiation treatment, marbling level, and storage time in Phase I. The differences were not observed for Phase II samples. Bacteriological assays indicated that irradiated samples were commercially sterile. Extremely low numbers of Micrococci were found. (Dissertation Abstr., 23: No. 4)« less

  9. Improving vaccination cold chain in the general practice setting.

    PubMed

    Page, Sue L; Earnest, Arul; Birden, Hudson; Deaker, Rachelle; Clark, Chris

    2008-10-01

    This study compared temperature control in different types of vaccine storing refrigerators in general practice and tested knowledge of general practice staff in vaccine storage requirements. Temperature data loggers were set to serially record the temperature within vaccine refrigerators in 28 general practices, recording at 12 minute intervals over a period of 10 days on each occasion. A survey of vaccine storage knowledge and records of divisions of general practice immunisation contacts were also obtained. There was a significant relationship between type of refrigerator and optimal temperature, with the odds ratio for bar style refrigerator being 0.005 (95% CI: 0.001-0.044) compared to the purpose built vaccine refrigerators. Score on a survey of vaccine storage was also positively associated with optimal storage temperature. General practices that invest in purpose built vaccine refrigerators will achieve standards of vaccine cold chain maintenance significantly more reliably than can be achieved through regular cold chain monitoring and practice supports.

  10. Artificial Permafrost and the Application to the Low Temperature Storage for Foodstuffs

    NASA Astrophysics Data System (ADS)

    Ryokai, Kimitoshi; Fukuda, Masami

    In the cold regions like Hokkaido and Tohoku Districts, they have been advocating snow-overcoming, advantages of snow and effective utilization of cold climate. In fact, they have been positively trying to make use of snow and coldness as water resources, energy sources, structural materials and so on. One of energy utilization is for low temperature storage of foods. Since the potatoes have properties of adapting themselves to cold temperature when they are stored under cold environment, they have the tendency of growing in their sugar contents. As the results, all those foods which are stored under these cold environments will be the products of higher additional value. Here we will introduce the present situation of low temperature storage of foods by artificial permafrost, not only as the construction materials for cold storage house itself but also utilizing its own cold temperature.

  11. Influence of Synaptic Depression on Memory Storage Capacity

    NASA Astrophysics Data System (ADS)

    Otsubo, Yosuke; Nagata, Kenji; Oizumi, Masafumi; Okada, Masato

    2011-08-01

    Synaptic efficacy between neurons is known to change within a short time scale dynamically. Neurophysiological experiments show that high-frequency presynaptic inputs decrease synaptic efficacy between neurons. This phenomenon is called synaptic depression, a short term synaptic plasticity. Many researchers have investigated how the synaptic depression affects the memory storage capacity. However, the noise has not been taken into consideration in their analysis. By introducing ``temperature'', which controls the level of the noise, into an update rule of neurons, we investigate the effects of synaptic depression on the memory storage capacity in the presence of the noise. We analytically compute the storage capacity by using a statistical mechanics technique called Self Consistent Signal to Noise Analysis (SCSNA). We find that the synaptic depression decreases the storage capacity in the case of finite temperature in contrast to the case of the low temperature limit, where the storage capacity does not change.

  12. Research on crude oil storage and transportation based on optimization algorithm

    NASA Astrophysics Data System (ADS)

    Yuan, Xuhua

    2018-04-01

    At present, the optimization theory and method have been widely used in the optimization scheduling and optimal operation scheme of complex production systems. Based on C++Builder 6 program development platform, the theoretical research results are implemented by computer. The simulation and intelligent decision system of crude oil storage and transportation inventory scheduling are designed. The system includes modules of project management, data management, graphics processing, simulation of oil depot operation scheme. It can realize the optimization of the scheduling scheme of crude oil storage and transportation system. A multi-point temperature measuring system for monitoring the temperature field of floating roof oil storage tank is developed. The results show that by optimizing operating parameters such as tank operating mode and temperature, the total transportation scheduling costs of the storage and transportation system can be reduced by 9.1%. Therefore, this method can realize safe and stable operation of crude oil storage and transportation system.

  13. Solid Aluminum Borohydrides for Prospective Hydrogen Storage.

    PubMed

    Dovgaliuk, Iurii; Safin, Damir A; Tumanov, Nikolay A; Morelle, Fabrice; Moulai, Adel; Černý, Radovan; Łodziana, Zbigniew; Devillers, Michel; Filinchuk, Yaroslav

    2017-12-08

    Metal borohydrides are intensively researched as high-capacity hydrogen storage materials. Aluminum is a cheap, light, and abundant element and Al 3+ can serve as a template for reversible dehydrogenation. However, Al(BH 4 ) 3 , containing 16.9 wt % of hydrogen, has a low boiling point, is explosive on air and has poor storage stability. A new family of mixed-cation borohydrides M[Al(BH 4 ) 4 ], which are all solid under ambient conditions, show diverse thermal decomposition behaviors: Al(BH 4 ) 3 is released for M=Li + or Na + , whereas heavier derivatives evolve hydrogen and diborane. NH 4 [Al(BH 4 ) 4 ], containing both protic and hydridic hydrogen, has the lowest decomposition temperature of 35 °C and yields Al(BH 4 ) 3 ⋅NHBH and hydrogen. The decomposition temperatures, correlated with the cations' ionic potential, show that M[Al(BH 4 ) 4 ] species are in the most practical stability window. This family of solids, with convenient and versatile properties, puts aluminum borohydride chemistry in the mainstream of hydrogen storage research, for example, for the development of reactive hydride composites with increased hydrogen content. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

  14. An experimental investigation of the effect of temperature and space velocity on the performance of a cu-zeolite flow-through SCR and a SCR catalyst on a DPF with and without PM loading

    NASA Astrophysics Data System (ADS)

    Kadam, Vaibhav

    The heavy-duty diesel (HDD) engines use the diesel oxidation catalyst (DOC), catalyzed particulate filter (CPF) and urea injection based selective catalytic reduction (SCR) systems in sequential combination, to meet the US EPA 2010 PM and NOx emission standards. The SCR along with a NH 3 slip control catalyst (AMOX) offer NOx reduction >90 % with NH3 slip <20 ppm. However, there is a strong desire to further improve the NOx reduction performance of such systems, to meet the California Optional Low NOx Standard implemented since 2015. Integrating SCR functionality into a diesel particulate filter (DPF), by coating the SCR catalyst on the DPF, offers potential to reduce the system cost and packaging weight/ volume. It also provides opportunity to increases the SCR volume without affecting the overall packaging, to achieve NO x reduction efficiencies >95 %. In this research, the NOx reduction and NH3 storage performance of a Cu-zeolite SCR and Cu-zeolite SCR catalyst on DPF (SCRFRTM) were experimentally investigated based on the engine experimental data at steady state conditions. The experimental data for the production-2013-SCR and the SCRFRTM were collected (with and without PM loading in the SCRFRTM) on a Cummins ISB 2013 engine, at varying inlet temperatures, space velocities, inlet NOx concentrations and NO2/NOx ratios, to evaluate the NOx reduction, NH3 storage and NH 3 slip characteristics of the SCR catalyst. The SCRFRTM was loaded with 2 and 4 g/L of PM prior to the NOx reduction tests to study the effect of PM loading on the NOx reduction and NH3 storage performance of the SCRFRTM. The experimental setup and test procedures for evaluation of NOx reduction performance of the SCRFRTM, with and without PM loading in the SCRFRTM are described. The 1-D SCR model developed at MTU was calibrated to the engine experimental data obtained from the seven NOx reduction tests conducted with the production-2013-SCR. The performance of the 1-D SCR model was validated by comparing the simulation and experimental data for NO, NO2 and NH3 concentrations at the outlet of the SCR. The NO and NO 2 concentrations were calibrated to +/-20 ppm and NH3 was calibrated to +/-20 ppm. The experimental results for the production-2013-SCR indicate that the NOx reduction of 80 - 85% can be achieved for the inlet temperatures below 250°C and above 450°C and NO x reduction of 90 - 95% can be achieved for the inlet temperatures between 300 - 350°C, at ammonia to NO2 ratio (ANR) 1.0, while the NH3 slip out of the SCR was <75 ppm. Conversely, the SCRFRTM showed 90 - 95 % NOx reduction at ANR of 1.0, while the NH3 slip out of the SCRFRTM was >50 ppm, with and without PM loading in the SCRFRTM, for the inlet temperature range of 200 - 450 °C, space velocity in the range of 13 to 48 k/hr and inlet NO 2/NOx in the range of 0.2 to 0.5. The NOx reduction in the SCRFRTM increases to >98 % at ANR 1.2. However, the NH3 slip out of the SCRFRTM increases significantly at ANR 1.2. The effect of PM loading at 2 and 4 g/L on the NOx reduction performance of the SCRFRTM was negligible below 300 °C. However, with PM loading in the SCRFRTM, the NO2 reduction decreased by 3 - 5% when compared to the clean SCRFRTM, for inlet temperature >350 °C. Experimental data were also collected by reference [1] to investigate the NO2 assisted PM oxidation in the SCRFRTM for the inlet temperature range of 260 - 370 °C, with and without urea injection and thermal oxidation of PM in the SCRFRTM for the inlet temperature range of 500 - 600 °C, without urea injection by reference [1]. The experimental data obtained from this study and [1] will be used to develop and calibrate the SCR-F model at Michigan Tech. The NH3 storage for the production-2013-SCR and the SCRFRTM (with and without PM loading) were determined from the steady state engine experimental data. The NH3 storage for the production-2013-SCR and the SCRFRTM (without PM loading) were within +/-5 gmol/m 3 of the substrate, with maximum NH3 storage of 75 - 80 gmol/m3 of the substrate, at the SCR/SCRFRTM inlet temperature of 200°C. The NH3 storage in the SCRFRTM, with 2 g/L PM loading, decreased by 30%, when compared to the NH3 storage in the SCRFRTM, without PM loading. The further increase in the PM loading in the SCRFRTM, from 2 to 4 g/L, had negligible effect on NH 3 storage.

  15. Study of Reactive Melt Processing Behavior of Externally Plasticized Cellulose Acetate in Presence of Isocyanate

    PubMed Central

    Erdmann, Rafael; Kabasci, Stephan; Kurek, Joanna; Zepnik, Stefan

    2014-01-01

    Two types of externally plasticized cellulose acetate (CA) were chemically modified using 4,4'-methylene diphenyl diisocyanate (MDI) as crosslinking agent. Crosslinking was performed in the molten state by means of melt mixing in an internal mixer. The viscoelastic properties of the non-crosslinked, externally plasticized CA show typical temperature dependence, similar to conventional thermoplastics. A strong increase in storage modulus is observed with increasing crosslink density indicating that the crosslinked compounds exhibit predominately elastic response. The complex viscosity also increases considerably with increasing crosslink density and does not reach the typical Newtonian plateau at low radial frequencies any more. The viscoelastic properties correlate well with the data recorded online during reactive melt processing in the internal mixer. In comparison to the non-crosslinked CA, the crosslinked compounds show higher glass transition temperature, higher VICAT softening temperatures, improved thermal stability and lower plasticizer evaporation at evaluated temperatures. PMID:28788273

  16. Study of Reactive Melt Processing Behavior of Externally Plasticized Cellulose Acetate in Presence of Isocyanate.

    PubMed

    Erdmann, Rafael; Kabasci, Stephan; Kurek, Joanna; Zepnik, Stefan

    2014-12-04

    Two types of externally plasticized cellulose acetate (CA) were chemically modified using 4,4'-methylene diphenyl diisocyanate (MDI) as crosslinking agent. Crosslinking was performed in the molten state by means of melt mixing in an internal mixer. The viscoelastic properties of the non-crosslinked, externally plasticized CA show typical temperature dependence, similar to conventional thermoplastics. A strong increase in storage modulus is observed with increasing crosslink density indicating that the crosslinked compounds exhibit predominately elastic response. The complex viscosity also increases considerably with increasing crosslink density and does not reach the typical Newtonian plateau at low radial frequencies any more. The viscoelastic properties correlate well with the data recorded online during reactive melt processing in the internal mixer. In comparison to the non-crosslinked CA, the crosslinked compounds show higher glass transition temperature, higher VICAT softening temperatures, improved thermal stability and lower plasticizer evaporation at evaluated temperatures.

  17. New insights into designing metallacarborane based room temperature hydrogen storage media.

    PubMed

    Bora, Pankaj Lochan; Singh, Abhishek K

    2013-10-28

    Metallacarboranes are promising towards realizing room temperature hydrogen storage media because of the presence of both transition metal and carbon atoms. In metallacarborane clusters, the transition metal adsorbs hydrogen molecules and carbon can link these clusters to form metal organic framework, which can serve as a complete storage medium. Using first principles density functional calculations, we chalk out the underlying principles of designing an efficient metallacarborane based hydrogen storage media. The storage capacity of hydrogen depends upon the number of available transition metal d-orbitals, number of carbons, and dopant atoms in the cluster. These factors control the amount of charge transfer from metal to the cluster, thereby affecting the number of adsorbed hydrogen molecules. This correlation between the charge transfer and storage capacity is general in nature, and can be applied to designing efficient hydrogen storage systems. Following this strategy, a search for the best metallacarborane was carried out in which Sc based monocarborane was found to be the most promising H2 sorbent material with a 9 wt.% of reversible storage at ambient pressure and temperature.

  18. New insights into designing metallacarborane based room temperature hydrogen storage media

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Bora, Pankaj Lochan; Singh, Abhishek K.

    Metallacarboranes are promising towards realizing room temperature hydrogen storage media because of the presence of both transition metal and carbon atoms. In metallacarborane clusters, the transition metal adsorbs hydrogen molecules and carbon can link these clusters to form metal organic framework, which can serve as a complete storage medium. Using first principles density functional calculations, we chalk out the underlying principles of designing an efficient metallacarborane based hydrogen storage media. The storage capacity of hydrogen depends upon the number of available transition metal d-orbitals, number of carbons, and dopant atoms in the cluster. These factors control the amount of chargemore » transfer from metal to the cluster, thereby affecting the number of adsorbed hydrogen molecules. This correlation between the charge transfer and storage capacity is general in nature, and can be applied to designing efficient hydrogen storage systems. Following this strategy, a search for the best metallacarborane was carried out in which Sc based monocarborane was found to be the most promising H{sub 2} sorbent material with a 9 wt.% of reversible storage at ambient pressure and temperature.« less

  19. How fragility makes phase-change data storage robust: insights from ab initio simulations

    PubMed Central

    Zhang, Wei; Ronneberger, Ider; Zalden, Peter; Xu, Ming; Salinga, Martin; Wuttig, Matthias; Mazzarello, Riccardo

    2014-01-01

    Phase-change materials are technologically important due to their manifold applications in data storage. Here we report on ab initio molecular dynamics simulations of crystallization of the phase change material Ag4In3Sb67Te26 (AIST). We show that, at high temperature, the observed crystal growth mechanisms and crystallization speed are in good agreement with experimental data. We provide an in-depth understanding of the crystallization mechanisms at the atomic level. At temperatures below 550 K, the computed growth velocities are much higher than those obtained from time-resolved reflectivity measurements, due to large deviations in the diffusion coefficients. As a consequence of the high fragility of AIST, experimental diffusivities display a dramatic increase in activation energies and prefactors at temperatures below 550 K. This property is essential to ensure fast crystallization at high temperature and a stable amorphous state at low temperature. On the other hand, no such change in the temperature dependence of the diffusivity is observed in our simulations, down to 450 K. We also attribute this different behavior to the fragility of the system, in combination with the very fast quenching times employed in the simulations. PMID:25284316

  20. Effect of storage temperature and duration on the behavior of Escherichia coli O157:H7 on packaged fresh-cut salad containing romaine and iceberg lettuce.

    PubMed

    Luo, Yaguang; He, Qiang; McEvoy, James L

    2010-09-01

    This study investigated the impact of storage temperature and duration on the fate of Escherichia coli O157:H7 on commercially packaged lettuce salads, and on product quality. Fresh-cut Romaine and Iceberg lettuce salads of different commercial brands were obtained from both retail and wholesale stores. The packages were cut open at one end, the lettuce salad inoculated with E. coli O157:H7 via a fine mist spray, and resealed with or without an initial N(2) flush to match the original package atmospheric levels. The products were stored at 5 and 12 °C until their labeled "Best If Used By" dates, and the microbial counts and product quality were monitored periodically. The results indicate that storage at 5 °C allowed E. coli O157:H7 to survive, but limited its growth, whereas storage at 12 °C facilitated the proliferation of E. coli O157:H7. There was more than 2.0 log CFU/g increase in E. coli O157:H7 populations on lettuce when held at 12 °C for 3 d, followed by additional growth during the remainder of the storage period. Although there was eventually a significant decline in visual quality of lettuce held at 12 °C, the quality of this lettuce was still fully acceptable when E. coli O157:H7 growth reached a statistically significant level. Therefore, maintaining fresh-cut products at 5 °C or below is critical for reducing the food safety risks as E. coli O157:H7 grows at a rapid, temperature-dependent rate prior to significant quality deterioration. Specific information regarding the effect of temperature on pathogen growth on leafy greens is needed to develop science-based food safety guidelines and practices by the regulatory agencies and produce industry. Temperature control is commonly thought to promote quality of leafy greens, not safety, based at least partially on a theory that product quality deterioration precedes pathogen growth at elevated temperatures. This prevalent attitude results in temperature abuse incidents being frequently overlooked in the supply chain. This study demonstrates that human pathogens, such as E. coli O157:H7, can grow significantly on commercially packaged lettuce salads while the product's visual quality is fully acceptable. Packaged fresh-cut salads are marketed as "ready-to-eat" while lacking an effective pathogen kill step during their preparation. Thus, maintaining storage temperature at 5 °C or below is critical to prevent pathogen proliferation and mitigate food safety risks should pathogen contamination inadvertently occur during crop growth or postharvest fresh-cut processing.

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