Sample records for injection project trail

  1. Farfield structure of an aircraft trailing vortex, including effects of mass injection

    NASA Technical Reports Server (NTRS)

    Mason, W. H.; Marchman, J. F., III

    1972-01-01

    Wind tunnel tests to predict the aircraft wake turbulence due to the tip trailing vortex are discussed. A yawhead pressure probe was used in a subsonic wind tunnel to obtain detailed mean flow measurements at stations up to 30 chordlengths downstream in an aircraft trailing vortex. Mass injection at the wingtip was shown to hasten the decay of the trailing vortex. A theoretical method is presented to show the effect which the circulation distribution on the wing has on the structure of the outer portion of the vortex.

  2. Effect of Trailing Edge Flow Injection on Fan Noise and Aerodynamic Performance

    NASA Technical Reports Server (NTRS)

    Fite, E. Brian; Woodward, Richard P.; Podboy, Gary G.

    2006-01-01

    An experimental investigation using trailing edge blowing for reducing fan rotor/guide vane wake interaction noise was completed in the NASA Glenn 9- by 15-foot Low Speed Wind Tunnel. Data were acquired to measure noise, aerodynamic performance, and flow features for a 22" tip diameter fan representative of modern turbofan technology. The fan was designed to use trailing edge blowing to reduce the fan blade wake momentum deficit. The test objective was to quantify noise reductions, measure impacts on fan aerodynamic performance, and document the flow field using hot-film anemometry. Measurements concentrated on approach, cutback, and takeoff rotational speeds as those are the primary conditions of acoustic interest. Data are presented for a 2% (relative to overall fan flow) trailing edge injection rate and show a 2 dB reduction in Overall Sound Power Level (OAPWL) at all fan test speeds. The reduction in broadband noise is nearly constant and is approximately 1.5 dB up to 20 kHz at all fan speeds. Measurements of tone noise show significant variation, as evidenced by reductions of up to 6 dB in the 2 BPF tone at 6700 rpm.: and increases of nearly 2 dB for the 4 BPF tone at approach speed. Aerodynamic performance measurements show the fan with 2 % injection has an overall efficiency that is comparable to the baseline fan and operates, as intended, with nearly the same pressure ratio and mass flow parameters. Hot-film measurements obtained at the approach operating condition indicate that mean blade wake filling in the tip region was not as significant as expected. This suggests that additional acoustic benefits could be realized if the trailing edge blowing could be modified to provide better filling of the wake momentum deficit. Nevertheless, the hot-film measurements indicate that the trailing edge blowing provided significant reductions in blade wake turbulence. Overall, these results indicate that further work may be required to fully understand the proper

  3. 75 FR 29359 - Draft Environmental Impact Statement for the Tamiami Trail Modifications: Next Steps Project...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-25

    ... Tamiami Trail Modifications: Next Steps Project, Everglades National Park Tamiami Trail Modifications... Interior. ACTION: Notice of availability of the Draft Environmental Impact Statement for the Tamiami Trail... Statement (DEIS) for the Tamiami Trail (U.S. Highway 41) Modifications: Next Steps Project for Everglades...

  4. Experimental evaluation of the certification-trail method

    NASA Technical Reports Server (NTRS)

    Sullivan, Gregory F.; Wilson, Dwight S.; Masson, Gerald M.; Itoh, Mamoru; Smith, Warren W.; Kay, Jonathan S.

    1993-01-01

    Certification trails are a recently introduced and promising approach to fault-detection and fault-tolerance. A comprehensive attempt to assess experimentally the performance and overall value of the method is reported. The method is applied to algorithms for the following problems: huffman tree, shortest path, minimum spanning tree, sorting, and convex hull. Our results reveal many cases in which an approach using certification-trails allows for significantly faster overall program execution time than a basic time redundancy-approach. Algorithms for the answer-validation problem for abstract data types were also examined. This kind of problem provides a basis for applying the certification-trail method to wide classes of algorithms. Answer-validation solutions for two types of priority queues were implemented and analyzed. In both cases, the algorithm which performs answer-validation is substantially faster than the original algorithm for computing the answer. Next, a probabilistic model and analysis which enables comparison between the certification-trail method and the time-redundancy approach were presented. The analysis reveals some substantial and sometimes surprising advantages for ther certification-trail method. Finally, the work our group performed on the design and implementation of fault injection testbeds for experimental analysis of the certification trail technique is discussed. This work employs two distinct methodologies, software fault injection (modification of instruction, data, and stack segments of programs on a Sun Sparcstation ELC and on an IBM 386 PC) and hardware fault injection (control, address, and data lines of a Motorola MC68000-based target system pulsed at logical zero/one values). Our results indicate the viability of the certification trail technique. It is also believed that the tools developed provide a solid base for additional exploration.

  5. CSX rail trestle conversion to ATV trail.

    DOT National Transportation Integrated Search

    2014-10-01

    The Hatfield and McCoy Trail Authority (HMRRA) operates the Rockhouse ATV trail : between Man and Gilbert WV. Prior to this project, trail riders wishing to cross the Guyandotte : River had to ride the streets of Man, WV. The project goal was to prom...

  6. Linking Learning Style Theory with Retention Research: The TRAILS Project. AIR Professional File.

    ERIC Educational Resources Information Center

    Kalsbeek, David H.

    The Trucking Retention and Academic Integration by Learning Style (TRAILS) research project at St. Louis University, which is designed to incorporate information on learning style in ongoing enrollment research and improve campus retention, is described. Learning style and the Tinto Model of student attrition are discussed, along with the…

  7. Systemic immunological tolerance to ocular antigens is mediated by TRAIL-expressing CD8+ T cells.

    PubMed

    Griffith, Thomas S; Brincks, Erik L; Gurung, Prajwal; Kucaba, Tamara A; Ferguson, Thomas A

    2011-01-15

    Systemic immunological tolerance to Ag encountered in the eye restricts the formation of potentially damaging immune responses that would otherwise be initiated at other anatomical locations. We previously demonstrated that tolerance to Ag administered via the anterior chamber (AC) of the eye required Fas ligand-mediated apoptotic death of inflammatory cells that enter the eye in response to the antigenic challenge. Moreover, the systemic tolerance induced after AC injection of Ag was mediated by CD8(+) regulatory T cells. This study examined the mechanism by which these CD8(+) regulatory T cells mediate tolerance after AC injection of Ag. AC injection of Ag did not prime CD4(+) T cells and led to increased TRAIL expression by splenic CD8(+) T cells. Unlike wild-type mice, Trail(-/-) or Dr5(-/-) mice did not develop tolerance to Ag injected into the eye, even though responding lymphocytes underwent apoptosis in the AC of the eyes of these mice. CD8(+) T cells from Trail(-/-) mice that were first injected via the AC with Ag were unable to transfer tolerance to naive recipient wild-type mice, but CD8(+) T cells from AC-injected wild-type or Dr5(-/-) mice could transfer tolerance. Importantly, the transferred wild-type (Trail(+/+)) CD8(+) T cells were also able to decrease the number of infiltrating inflammatory cells into the eye; however, Trail(-/-) CD8(+) T cells were unable to limit the inflammatory cell ingress. Together, our data suggest that "helpless" CD8(+) regulatory T cells generated after AC injection of Ag enforce systemic tolerance in a TRAIL-dependent manner to inhibit inflammation in the eye.

  8. 77 FR 29624 - Cheniere Creole Trail Pipeline, L.P.; Notice of Application

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-18

    ... Creole Trail Pipeline, L.P.; Notice of Application Take notice that on April 30, 2012, Cheniere Creole Trail Pipeline, L.P. (Cheniere), 700 Milam, Suite 800, Houston, TX 77002, filed an application in Docket... Creole Trail Expansion Project (Project). Cheniere's Project would consist of construction of a new 53...

  9. Snails and their trails: the multiple functions of trail-following in gastropods.

    PubMed

    Ng, Terence P T; Saltin, Sara H; Davies, Mark S; Johannesson, Kerstin; Stafford, Richard; Williams, Gray A

    2013-08-01

    discrimination, including the mechanisms by which many snails determine the polarity of the trail, are yet to be experimentally determined. Given the multiple functions of trail-following we propose that future studies should adopt an integrated approach, taking into account the possibility of the simultaneous occurrence of many selectively advantageous roles of trail-following behaviour in gastropods. We also believe that future opportunities to link phenotypic and genotypic traits will make possible a new generation of research projects in which gastropod trail-following, its multitude of functions and evolutionary trade-offs can be further elucidated. © 2013 The Authors. Biological Reviews © 2013 Cambridge Philosophical Society.

  10. Linking Learning Style Theory with Retention Research: The TRAILS Project. AIR 1986 Annual Forum Paper.

    ERIC Educational Resources Information Center

    Kalsbeek, David H.

    The Myers-Briggs Type Indicator (MBTI), a measure of personality type and learning style, was used at Saint Louis University in the TRAILS (Tracking Retention and Academic Integration by Learning Style) Project. In addition to considering links between learning styles and student academic achievement and aptitude, MBTI was used to identify…

  11. Na Ala Hele (Trails for Walking).

    ERIC Educational Resources Information Center

    Hawaii State Dept. of Planning and Economic Development, Honolulu.

    This proposal for the development of a system of administering hiking trails in the state of Hawaii when such trails would involve various public and private jurisdictions emphasizes three elements: (a) proposing means of administration involving multiple jurisdictions; (b) demonstrating by means of a proposed project on the west coast of the Big…

  12. Evaluation of a Terminal Area In-Trail Approach Spacing, Project and Study

    NASA Technical Reports Server (NTRS)

    Shelden, Stephen; Johnson, Walter (Editor)

    2001-01-01

    Reported here are the results of work completed as a precursor to Distributed Air Ground (DAG), Concept Element 11 (CE11) research. CE11 is a NASA, Advanced Air Transportation Technologies (AATT) concept initiated to promote research on in-trail merging and spacing during approach in the terminal area environment, such that improvements to the National Air Space (NAS) may be realized. A description of the project concept, results of a preliminary study, and a literature review are presented. In terms of conclusions, study results, and reference material respectively: 1) the concept is supported as being significant to NAS capacity improvement, 2) the preliminary study indicated that having more than one downstream, in-trail aircraft present on a CDTI during approach may be advantageous, and that flying traditional Standard Terminal Arrival Routes (STAR) utilizing advanced decision support tools (DST) may be complicated by wake turbulence considerations, a lack of vertical awareness on the part of the flight crew, and the 'step-down' nature of many terminal area approaches, and 3) the results of a literature review are presented for future reference.

  13. A Tale of Two Trails: Exploring Different Paths to Success

    PubMed Central

    Walker, Jennifer G.; Evenson, Kelly R.; Davis, William J.; Bors, Philip; Rodríguez, Daniel A.

    2016-01-01

    Background This comparative case study investigates 2 successful community trail initiatives, using the Active Living By Design (ALBD) Community Action Model as an analytical framework. The model includes 5 strategies: preparation, promotion, programs, policy, and physical projects. Methods Key stakeholders at 2 sites participated in in-depth interviews (N = 14). Data were analyzed for content using Atlas Ti and grouped according to the 5 strategies. Results Preparation Securing trail resources was challenging, but shared responsibilities facilitated trail development. Promotions The initiatives demonstrated minimal physical activity encouragement strategies. Programs Community stakeholders did not coordinate programmatic opportunities for routine physical activity. Policy Trails’ inclusion in regional greenway master plans contributed to trail funding and development. Policies that were formally institutionalized and enforced led to more consistent trail construction and safer conditions for users. Physical Projects Consistent standards for way finding signage and design safety features enhanced trail usability and safety. Conclusions Communities with different levels of government support contributed unique lessons to inform best practices of trail initiatives. This study revealed a disparity between trail development and use-encouragement strategies, which may limit trails’ impact on physical activity. The ALBD Community Action Model provided a viable framework to structure cross-disciplinary community trail initiatives. PMID:21597125

  14. Trail Transect: A Method for Documenting Trail Changes

    Treesearch

    R.E. Leonard; A.M. Whitney

    1977-01-01

    A trail transect that documents trail conditions and changes in trails has been used successfully over the past 3 years to measure soil loss, and changes in trail width and vegetation. The system also can be used to study the relationship between trail degradation and the physical characteristics of the site. Data from trail transects can be useful in determining the...

  15. Argentine ant trail pheromone disruption is mediated by trail concentration.

    PubMed

    Suckling, David Maxwell; Stringer, Lloyd D; Corn, Joshua E

    2011-10-01

    Argentine ant trail pheromone disruption, using continuous release of the trail pheromone compound (Z)-9-hexadecanal, reduces the incidence of trails and foraging rates of field populations. However, little is known about the concentrations of pheromone required for successful disruption. We hypothesized that higher pheromone quantities would be necessary to disrupt larger ant populations. To test this, we laid a 30-cm long base trail of (Z)-9-hexadecanal on a glass surface at low and high rates (1 and 100 pg/cm) (Trail 1), and laid a second, shorter trail (Trail 2, 10 cm long, located 1.5 cm upwind) near the middle of Trail 1 at six rates (1, 10, 100, 1,000, 10,000, and 100,000 pg/cm). We then recorded and digitized movements of individual ants following Trail 1, and derived a regression statistic, r (2), as an index of trail integrity, and also recorded arrival success at the other end of the trail (30 cm) near a food supply. Disruption of trails required 100 fold more pheromone upwind, independent of base-trail concentration. This implies that in the field, trail disruption is likely to be less successful against high ant-trail densities (greater concentration of trail pheromone), and more successful against newly formed or weak trails, as could be expected along invasion fronts.

  16. Nanoparticle engineered TRAIL-overexpressing adipose-derived stem cells target and eradicate glioblastoma via intracranial delivery

    PubMed Central

    Jiang, Xinyi; Fitch, Sergio; Wang, Christine; Wilson, Christy; Li, Jianfeng; Grant, Gerald A.; Yang, Fan

    2016-01-01

    Glioblastoma multiforme (GBM) is one of the most intractable of human cancers, principally because of the highly infiltrative nature of these neoplasms. Tracking and eradicating infiltrating GBM cells and tumor microsatellites is of utmost importance for the treatment of this devastating disease, yet effective strategies remain elusive. Here we report polymeric nanoparticle-engineered human adipose-derived stem cells (hADSCs) overexpressing tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) as drug-delivery vehicles for targeting and eradicating GBM cells in vivo. Our results showed that polymeric nanoparticle-mediated transfection led to robust up-regulation of TRAIL in hADSCs, and that TRAIL-expressing hADSCs induced tumor-specific apoptosis. When transplanted in a mouse intracranial xenograft model of patient-derived glioblastoma cells, hADSCs exhibited long-range directional migration and infiltration toward GBM tumor. Importantly, TRAIL-overexpressing hADSCs inhibited GBM growth, extended survival, and reduced the occurrence of microsatellites. Repetitive injection of TRAIL-overexpressing hADSCs significantly prolonged animal survival compared with single injection of these cells. Taken together, our data suggest that nanoparticle-engineered TRAIL-expressing hADSCs exhibit the therapeutically relevant behavior of “seek-and-destroy” tumortropic migration and could be a promising therapeutic approach to improve the treatment outcomes of patients with malignant brain tumors. PMID:27849590

  17. Exploring the TRAILs less travelled: TRAIL in cancer biology and therapy.

    PubMed

    von Karstedt, Silvia; Montinaro, Antonella; Walczak, Henning

    2017-05-24

    The discovery that the tumour necrosis factor-related apoptosis-inducing ligand (TRAIL) can induce apoptosis of cancer cells without causing toxicity in mice has led to the in-depth study of pro-apoptotic TRAIL receptor (TRAIL-R) signalling and the development of biotherapeutic drug candidates that activate TRAIL-Rs. The outcome of clinical trials with these TRAIL-R agonists has, however, been disappointing so far. Recent evidence indicates that many cancers, in addition to being TRAIL resistant, use the endogenous TRAIL-TRAIL-R system to their own advantage. However, novel insight on two fronts - how resistance of cancer cells to TRAIL-based pro-apoptotic therapies might be overcome, and how the pro-tumorigenic effects of endogenous TRAIL might be countered - gives reasonable hope that the TRAIL system can be harnessed to treat cancer. In this Review we assess the status quo of our understanding of the biology of the TRAIL-TRAIL-R system - as well as the gaps therein - and discuss the opportunities and challenges in effectively targeting this pathway.

  18. Efforts of a Kansas foundation to increase physical activity and improve health by funding community trails, 2012.

    PubMed

    Heinrich, Katie M; Lightner, Joseph; Oestman, Katherine B; Hughey, S Morgan; Kaczynski, Andrew T

    2014-11-26

    Trails are associated with increased physical activity; however, little is known about the process of building trails by various types of organizations. From 2005 through 2012 the Sunflower Foundation: Health Care for Kansans (Sunflower) funded multiple organizations to construct 70 trails of varying lengths and surfaces in municipalities, schools, and communities across Kansas. The purpose of this study was to assess the process of developing and implementing community trail projects across Kansas with funding from a public foundation. In 2012, we stratified funded organizations by type and conducted proportional random sampling to select 20 key informants from those organizations to participate in structured telephone interviews. Interviews were recorded and transcribed verbatim. Two researchers coded interview transcripts according to issues identified by participants. Issues associated with trail-building identified as important were collaboration among groups, unexpected construction costs, champions for the project, and level of difficulty of construction. Participants indicated that trails facilitated physical activity. Trails were integrated into communities through events such as walking events and other promotional efforts; these efforts were thought to increase trail use. The perceived outcomes of building the trails included providing the community with a physical activity resource, inspiring the community to start additional trail projects, and increasing the physical activity of local residents. Sunflower's funding was instrumental in developing trail projects to provide new physical activity resources across Kansas. Public health practitioners seeking to increase physical activity should seek funding from foundations that focus on health.

  19. 77 FR 58826 - Cheniere Creole Trail Pipeline, L.P.; Notice of Availability of the Environmental Assessment for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-24

    ... Creole Trail Pipeline, L.P.; Notice of Availability of the Environmental Assessment for the Proposed Creole Trail Expansion Project The staff of the Federal Energy Regulatory Commission (FERC or Commission) has prepared an environmental assessment (EA) for the Creole Trail Expansion Project, proposed by...

  20. 77 FR 36525 - Cheniere Creole Trail Pipeline Company, L.P.; Notice of Intent To Prepare an Environmental...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-19

    ... Creole Trail Pipeline Company, L.P.; Notice of Intent To Prepare an Environmental Assessment for the Proposed Creole Trail Expansion Project and Request for Comments on Environmental Issues The staff of the...) that will discuss the environmental impacts of the Creole Trail Expansion Project involving...

  1. Correlates of Trail Use for Recreation and Transportation on 5 Massachusetts Trails.

    PubMed

    Orstad, Stephanie L; McDonough, Meghan H; Klenosky, David B; Mattson, Marifran; Troped, Philip J

    2016-08-01

    Promoting use of community trails is a recommended strategy for increasing population levels of physical activity. Correlates of walking and cycling for recreation or transportation differ, though few studies have compared correlates of trail-based physical activity for recreation and transportation purposes. This study examined associations of demographic, social, and perceived built environmental factors with trail use for recreation and transportation and whether associations were moderated by age, gender, and prior trail use. Adults (N = 1195) using 1 of 5 trails in Massachusetts responded to an intercept survey. We used multiple linear and logistic regression models to examine associations with trail use. Respondents' mean age was 44.9 years (standard deviation = 12.5), 55.3% were female, and 82.0% were white. Age (longer-term users only), trail use with others, travel time to the trail, and trail design were significantly associated with use for recreation (P < .05). Age, gender, trail safety (longer-term users only), travel time to the trail, trail design (younger users only), and trail beauty were associated with use for transportation (P < .05). Some common correlates were found for recreational and transportation trail use, whereas some variables were uniquely associated with use for 1 purpose. Tailored strategies are suggested to promote trail use for recreation and transportation.

  2. Efforts of a Kansas Foundation to Increase Physical Activity and Improve Health by Funding Community Trails, 2012

    PubMed Central

    Heinrich, Katie M.; Lightner, Joseph; Oestman, Katherine B.; Kaczynski, Andrew T.

    2014-01-01

    Introduction Trails are associated with increased physical activity; however, little is known about the process of building trails by various types of organizations. From 2005 through 2012 the Sunflower Foundation: Health Care for Kansans (Sunflower) funded multiple organizations to construct 70 trails of varying lengths and surfaces in municipalities, schools, and communities across Kansas. The purpose of this study was to assess the process of developing and implementing community trail projects across Kansas with funding from a public foundation. Methods In 2012, we stratified funded organizations by type and conducted proportional random sampling to select 20 key informants from those organizations to participate in structured telephone interviews. Interviews were recorded and transcribed verbatim. Two researchers coded interview transcripts according to issues identified by participants. Results Issues associated with trail-building identified as important were collaboration among groups, unexpected construction costs, champions for the project, and level of difficulty of construction. Participants indicated that trails facilitated physical activity. Trails were integrated into communities through events such as walking events and other promotional efforts; these efforts were thought to increase trail use. The perceived outcomes of building the trails included providing the community with a physical activity resource, inspiring the community to start additional trail projects, and increasing the physical activity of local residents. Conclusion Sunflower’s funding was instrumental in developing trail projects to provide new physical activity resources across Kansas. Public health practitioners seeking to increase physical activity should seek funding from foundations that focus on health. PMID:25427316

  3. Restoration of multiple-rut trails in the Tuolumne meadows of Yosemite National Park

    Treesearch

    Sean Eagan; Peter Newman; Susan Fritzke; Louise Johnson

    2000-01-01

    This study presents the techniques used in a restoration project in Tuolumne Meadows on the old Glen Aulin trail in Yosemite National Park from 1990 to 1994 and the results of follow-up monitoring in the summer of 1998. The project restored the natural hydrology and soils to a 4,200-foot section of abandoned trail which had two to six one-foot deep ruts. The project...

  4. 76 FR 12212 - Notice of Availability of the Finding of No Significant Impact for the Klingle Valley Trail

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-04

    ... Significant Impact for the Klingle Valley Trail AGENCIES: Federal Highway Administration, District of Columbia...: Notice of availability of the Finding of No Significant Impact for the Klingle Valley Trail Project... availability of the Finding of No Significant Impact (FONSI) for the Klingle Valley Trail Project, pursuant to...

  5. Pre-test data and lessons learned from a group research project examining changes in physical activity behavior following construction of a rails-to-trails facility.

    PubMed

    Chatfield, Sheryl L; Mumaw, Elizabeth; Davis, T; Hallam, Jeffrey S

    2014-04-01

    Built environments in rural settings may provide greater challenges than those in urban settings due to physical characteristics inherent to low-density population areas. Multiuse recreational trails, such as those that repurpose abandoned railroad lines, may provide a physical activity resource that is well suited to rural areas. However, the direct impact of trail availability on physical activity behavior is not generally known because it is unclear whether activity reported in most trail research represents increases in physical activity or displacement of activity in individuals who previously exercised in other locations. This research, initiated by a group of students in a graduate seminar, represents to our knowledge, the first instance in which PA was assessed prior to the availability of an entirely new rails-to-trails facility. The research was implemented using a nonequivalent dependent variable design to counter the lack of a control group; the nonequivalent dependent variable chosen was weekly servings of fruit and vegetables. Participants responding to intercept interviews classified days of activity during the prior week as mild, moderate or vigorous. Baseline results for 244 participants suggested generally low levels of activity prior to trail availability; number of reported days of activity decreased with described intensity. We also discuss several issues encountered in planning and implementing this group project including those related to data collection, variable levels of commitment among student members, and inconsistent project management, and offer potential solutions to these concerns.

  6. 76 FR 26336 - Notice of Final Federal Agency Actions on the Proposed Klingle Valley Trail in Washington, DC

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-06

    ... on the Proposed Klingle Valley Trail in Washington, DC AGENCY: Federal Highway Administration (FHWA... final within the meaning of 23 U.S.C. 139(l)(1). The actions relate to the proposed Klingle Valley Trail... approvals for the following project in Northwest Washington, DC: The Klingle Valley Trail project. The...

  7. Perceptual Geography through Urban Trails.

    ERIC Educational Resources Information Center

    Dove, Jane

    1997-01-01

    Describes a project whereby geography students were charged with designing an urban trail (city walk with informational markers) that would accommodate specific groups. Chosen groups included people with physical disabilities, 10-year olds, and those interested in local street art. Discusses the cognitive, psychomotor, and affective objectives of…

  8. GAS INJECTION/WELL STIMULATION PROJECT

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    John K. Godwin

    2005-12-01

    Driver Production proposes to conduct a gas repressurization/well stimulation project on a six well, 80-acre portion of the Dutcher Sand of the East Edna Field, Okmulgee County, Oklahoma. The site has been location of previous successful flue gas injection demonstration but due to changing economic and sales conditions, finds new opportunities to use associated natural gas that is currently being vented to the atmosphere to repressurize the reservoir to produce additional oil. The established infrastructure and known geological conditions should allow quick startup and much lower operating costs than flue gas. Lessons learned from the previous project, the lessons learnedmore » form cyclical oil prices and from other operators in the area will be applied. Technology transfer of the lessons learned from both projects could be applied by other small independent operators.« less

  9. 76 FR 41323 - Notice of Availability of the Final Environmental Assessment for the Metropolitan Branch Trail

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-13

    ... Environmental Assessment for the Metropolitan Branch Trail AGENCY: Federal Highway Administration, District of.... ACTION: Notice of availability of the Final Environmental Assessment for the Metropolitan Branch Trail... availability of the Final Environmental Assessment (Ea) for the Metropolitan Branch Trail Project, pursuant to...

  10. Hiking trails and tourism impact assessment in protected area: Jiuzhaigou Biosphere Reserve, China.

    PubMed

    Li, Wenjun; Ge, Xiaodong; Liu, Chunyan

    2005-09-01

    More and more visitors are attracted to protected areas nowadays, which not only bring about economic increase but also seriously adverse impacts on the ecological environment. In protected areas, trails are linkage between visitors and natural ecosystem, so they concentrate most of the adverse impacts caused by visitors. The trampling problems on the trails have been received attentions in the tremendous researches. However, few of them have correlated the environmental impacts to trail spatial patterns. In this project, the trails were selected as assessment objective, the trampling problems trail widening, multiple trail, and root exposure were taken as assessment indicators to assess ecological impacts in the case study area Jiuzhaigou Biosphere Reserve, and two spatial index, connectivity and circularity, were taken to indicate the trail network spatial patterns. The research results showed that the appearing frequency of the trampling problems had inverse correlation with the circularity and connectivity of the trail network, while the problem extent had no correlation with the spatial pattern. Comparing with the pristine trails, the artificial maintenance for the trails such as wooden trails and flagstone trails could prohibit vegetation root from exposure effectively. The research finds will be useful for the future trail design and tourism management.

  11. DIRBE Comet Trails

    NASA Technical Reports Server (NTRS)

    Arendt, Richard G.

    2015-01-01

    Re-examination of the COBE DIRBE data reveals the thermal emission of several comet dust trails.The dust trails of 1P/Halley, 169P/NEAT, and 3200 Phaethon have not been previously reported.The known trails of 2P/Encke, and 73P/Schwassmann-Wachmann 3 are also seen. The dust trails have 12 and 25 microns surface brightnesses of <0.1 and <0.15 MJy/sr, respectively, which is <1% of the zodiacal light intensity. The trails are very difficult to see in any single daily image of the sky, but are evident as rapidly moving linear features in movies of the DIRBE data. Some trails are clearest when crossing through the orbital plane of the parent comet, but others are best seen at high ecliptic latitudes as the Earth passes over or under the dust trail. All these comets have known associations with meteor showers. This re-examination also reveals one additional comet and 13 additional asteroids that had not previously been recognized in the DIRBE data.

  12. Investigation of trailing mass in Z-pinch implosions and comparison to experiment

    NASA Astrophysics Data System (ADS)

    Yu, Edmund

    2007-11-01

    Wire-array Z pinches represent efficient, high-power x-ray sources with application to inertial confinement fusion, high energy density plasmas, and laboratory astrophysics. The first stage of a wire-array Z pinch is described by a mass ablation phase, during which stationary wires cook off material, which is then accelerated radially inwards by the JxB force. The mass injection rate varies axially and azimuthally, so that once the ablation phase concludes, the subsequent implosion is highly 3D in nature. In particular, a network of trailing mass and current is left behind the imploding plasma sheath, which can significantly affect pinch performance. In this work we focus on the implosion phase, electing to model the mass ablation via a mass injection scheme. Such a scheme has a number of injection parameters, but this freedom also allows us to gain understanding into the nature of the trailing mass network. For instance, a new result illustrates the role of azimuthal correlation. For an implosion which is 100% azimuthally correlated (corresponding to an azimuthally symmetric 2D r-z problem), current is forced to flow on the imploding plasma sheath, resulting in strong Rayleigh-Taylor (RT) growth. If, however, the implosion is not azimuthally symmetric, the additional azimuthal degree of freedom opens up new conducting paths of lower magnetic energy through the trailing mass network, effectively reducing RT growth. Consequently the 3D implosion experiences lower RT growth than the 2D r-z equivalent, and actually results in a more shell-like implosion. A second major goal of this work is to constrain the injection parameters by comparison to a well-diagnosed experimental data set, in which array mass was varied. In collaboration with R. Lemke, M. Desjarlais, M. Cuneo, C. Jennings, D. Sinars, E. Waisman

  13. The effect of a trail use intervention on urban trail use in Southern Nevada.

    PubMed

    Clark, Sheila; Bungum, Tim; Shan, Guogen; Meacham, Mindy; Coker, Lisa

    2014-10-01

    Communities are building or improving trail networks for biking and walking to encourage physical activity, but the relationship between trail environments and physical activity is not well understood. We examined the effect of a trail use intervention in Southern Nevada. We monitored the usage of urban trails (n=10) in Southern Nevada before, during, and after an intervention which included a marketing campaign promoting trail use and the addition of way-finding and incremental distance signage to selected trails (October 2011-October 2012). Data were collected with infrared monitors placed on the trails for three periods of 7days. We compared pre-, mid-, and post-intervention usage rates on the 6 trails where signage was added to usage rates on the 4 control trails. The groups of trails experienced different patterns of increases and decreases over the 1-year study period. Mean users per hour increased 31% for the study trails and 35% for the control trails (p<0.001), but the total increase did not vary between the groups. Trail use increased about 33% during the 1-year study period for the intervention. Adding wayfinding and incremental distance signage appeared to support the increase in usage which followed the marketing campaign. Copyright © 2014 Elsevier Inc. All rights reserved.

  14. DRBE comet trails

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Arendt, Richard G., E-mail: Richard.G.Arendt@nasa.gov

    2014-12-01

    Re-examination of the Cosmic Background Explorer Diffuse Infrared Background Experiment (DIRBE) data reveals the thermal emission of several comet dust trails. The dust trails of 1P/Halley, 169P/NEAT, and 3200 Phaethon have not been previously reported. The known trails of 2P/Encke and 73P/Schwassmann–Wachmann 3 are also seen. The dust trails have 12 and 25 μm surface brightnesses of <0.1 and <0.15 MJy sr{sup −1}, respectively, which is <1% of the zodiacal light intensity. The trails are very difficult to see in any single daily image of the sky, but are evident as rapidly moving linear features in movies of the DIRBEmore » data. Some trails are clearest when crossing through the orbital plane of the parent comet, but others are best seen at high ecliptic latitudes as the Earth passes over or under the dust trail. All these comets have known associations with meteor showers. This re-examination also reveals 1 additional comet and 13 additional asteroids that had not previously been recognized in the DIRBE data.« less

  15. SURVIAC Bulletin: AFRL Research Audit Trail Viewer (ATV). Volume 19, Issue 1, 2003

    DTIC Science & Technology

    2003-01-01

    Trail Viewer, the analyst obtained a close up view of the detailed aircraft model using the Orbit View, enabled the SkyBox , enabled fictional ter...trails and element projections, several simulated terrain types and Skybox environments to help the user maintain perspective, file based

  16. Promoting and developing a trail network across suburban, rural, and urban communities.

    PubMed

    Schasberger, Michele G; Hussa, Carol S; Polgar, Michael F; McMonagle, Julie A; Burke, Sharon J; Gegaris, Andrew J

    2009-12-01

    The Wyoming Valley Wellness Trails Partnership received an Active Living by Design grant late in 2003 for a project centered on a growing trail network linking urban, suburban, and rural communities in northeast Pennsylvania, a former coal region, in order to increase physical activity among residents. The partnership conducted research, collected information, created promotional documents, worked with partners on events and programs, and participated in trail planning. Local trail organizations continued planning and construction toward developing a trail network. Other partners spearheaded policy change in schools and worksites and worked toward downtown revitalization. The partnership assisted these efforts by providing a forum in which organizations could meet. The partnership became a central resource for information about local parks, trails, and outdoor recreational activities. The partnership increased awareness and use of recreational facilities. Trail partners constructed 22 miles of walking and biking trails. The partnership took advantage of an allied effort that created organizational capacity for wellness in schools and worksites. Messages promoting social and entertainment benefits of physical activity were more successful than those promoting health benefits. The existence of multiple small, independent trail organizations can help advance trail development through concurrent development efforts. Urban, suburban, and rural residents' conceptions of walkability may differ. Trails provide options for recreational and transportation-related physical activity across urban, suburban, and rural landscapes that are supported by all constituents. Trail builders can be strong allies in bringing active living to suburban and rural places.

  17. On the TRAIL to successful cancer therapy? Predicting and counteracting resistance against TRAIL-based therapeutics

    PubMed Central

    Dimberg, Lina Y.; Anderson, Charles K.; Camidge, Ross; Behbakht, Kian; Thorburn, Andrew; Ford, Heide L.

    2015-01-01

    TRAIL and agonistic antibodies against TRAIL death receptors kill tumor cells while causing virtually no damage to normal cells. Several novel drugs targeting TRAIL receptors are currently in clinical trials. However, TRAIL resistance is a common obstacle in TRAIL based therapy and limits the efficiency of these drugs. In this review article we discuss different mechanisms of TRAIL resistance and how they can be predicted and therapeutically circumvented. In addition, we provide a brief overview of all TRAIL based clinical trials conducted so far. It is apparent that although the effects of TRAIL therapy are disappointingly modest overall, a small subset of patients responds very well to TRAIL. We argue that the true potential of targeting TRAIL death receptors in cancer can only be reached when we find efficient ways to select for those patients that are most likely to benefit from the treatment. To achieve this, it is crucial to identify biomarkers that can help us predict TRAIL sensitivity. PMID:22580613

  18. Overview and Status of the Los Alamos PSR Injection Upgrade Project

    NASA Astrophysics Data System (ADS)

    Fitzgerald, D. H.; Ahn, H.; Blind, B.; Borden, M. J.; Macek, R. J.; Neri, F.; Rose, C. R.; Thiessen, H. A.; Wilkinson, C. A.; Zumbro, M. V.

    1997-05-01

    An upgrade is in progress to the Los Alamos Proton Storage Ring (PSR) to allow direct injection of the H^- beam into the ring and provide a beam bump system to move the circulating beam off the stripper foil. The primary benefits of this upgrade are matching the transverse phase space of the injected beam to the PSR acceptance and reduction of foil hits by the circulating beam by a factor of ten. Foil thickness is optimized to minimize the combination of circulating-beam losses plus losses due to excited H^0 states produced at injection. An overall factor of four reduction in losses is expected. The project comprises extensive modifications of the injection line, the injection section of the ring, and the waste-beam transport line. We will discuss the goals of the project, present an overview of the technical design, and describe the status of the implementation plan.

  19. Ohio trail users

    Treesearch

    Roger E. McCay

    1976-01-01

    Hikers, horseback riders, bicycle riders, and motorcycle riders were interviewed on randomly selected trails in Ohio to better understand who they are and why they use trails. Bicycle riders were found to be the most active trail users; bicycle and motorcycle riders were younger than hikers and horseback riders. The majority of hikers and horseback riders preferred...

  20. The science of trail surveys: Recreation ecology provides new tools for managing wilderness trails

    USGS Publications Warehouse

    Marion, Jeffrey L.; Wimpey, Jeremy F.; Park, Logan O.

    2011-01-01

    Recreation ecology examines the effects of recreation on protected area ecosystems. One core focus of recreation ecology research is trail science, including the development of efficient protocols to assess and monitor the type and severity of resource impacts, analyses to improve knowledge of factors that influence trail conditions, and studies to assist land managers in improving trail design, maintenance, and visitor management. This article reviews alternative trail survey methodologies most useful for the management of wilderness and backcountry trail networks. Illustrations and implications from survey data for trail planning, design, and management are included.

  1. Thigmotaxis Mediates Trail Odour Disruption.

    PubMed

    Stringer, Lloyd D; Corn, Joshua E; Sik Roh, Hyun; Jiménez-Pérez, Alfredo; Manning, Lee-Anne M; Harper, Aimee R; Suckling, David M

    2017-05-10

    Disruption of foraging using oversupply of ant trail pheromones is a novel pest management application under investigation. It presents an opportunity to investigate the interaction of sensory modalities by removal of one of the modes. Superficially similar to sex pheromone-based mating disruption in moths, ant trail pheromone disruption lacks an equivalent mechanistic understanding of how the ants respond to an oversupply of their trail pheromone. Since significant compromise of one sensory modality essential for trail following (chemotaxis) has been demonstrated, we hypothesised that other sensory modalities such as thigmotaxis could act to reduce the impact on olfactory disruption of foraging behaviour. To test this, we provided a physical stimulus of thread to aid trailing by Argentine ants otherwise under disruptive pheromone concentrations. Trail following success was higher using a physical cue. While trail integrity reduced under continuous over-supply of trail pheromone delivered directly on the thread, provision of a physical cue in the form of thread slightly improved trail following and mediated trail disruption from high concentrations upwind. Our results indicate that ants are able to use physical structures to reduce but not eliminate the effects of trail pheromone disruption.

  2. Targeting Death Receptor TRAIL-R2 by Chalcones for TRAIL-Induced Apoptosis in Cancer Cells

    PubMed Central

    Szliszka, Ewelina; Jaworska, Dagmara; Kłósek, Małgorzata; Czuba, Zenon P.; Król, Wojciech

    2012-01-01

    Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) induces apoptosis in cancer cells without toxicity to normal cells. TRAIL binds to death receptors, TRAIL-R1 (DR4) and TRAIL-R2 (DR5) expressed on cancer cell surface and activates apoptotic pathways. Endogenous TRAIL plays an important role in immune surveillance and defense against cancer cells. However, as more tumor cells are reported to be resistant to TRAIL mediated death, it is important to search for and develop new strategies to overcome this resistance. Chalcones can sensitize cancer cells to TRAIL-induced apoptosis. We examined the cytotoxic and apoptotic effects of TRAIL in combination with four chalcones: chalcone, isobavachalcone, licochalcone A and xanthohumol on HeLa cancer cells. The cytotoxicity was measured by MTT and LDH assays. The apoptosis was detected using annexin V-FITC staining by flow cytometry and fluorescence microscopy. Death receptor expression was analyzed using flow cytometry. The decreased expression of death receptors in cancer cells may be the cause of TRAIL-resistance. Chalcones enhance TRAIL-induced apoptosis in HeLa cells through increased expression of TRAIL-R2. Our study has indicated that chalcones augment the antitumor activity of TRAIL and confirm their cancer chemopreventive properties. PMID:23203129

  3. 76 FR 60115 - Notice of Availability of the Finding of No Significant Impact for the Metropolitan Branch Trail

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-28

    ... Significant Impact for the Metropolitan Branch Trail AGENCIES: Federal Highway Administration, District of.... ACTION: Notice of availability of the Finding of No Significant Impact for the Metropolitan Branch Trail... availability of the Finding of No Significant Impact (FONSI) for the Metropolitan Branch Trail Project...

  4. Certification trails for data structures

    NASA Technical Reports Server (NTRS)

    Sullivan, Gregory F.; Masson, Gerald M.

    1993-01-01

    Certification trails are a recently introduced and promising approach to fault detection and fault tolerance. The applicability of the certification trail technique is significantly generalized. Previously, certification trails had to be customized to each algorithm application; trails appropriate to wide classes of algorithms were developed. These certification trails are based on common data-structure operations such as those carried out using these sets of operations such as those carried out using balanced binary trees and heaps. Any algorithms using these sets of operations can therefore employ the certification trail method to achieve software fault tolerance. To exemplify the scope of the generalization of the certification trail technique provided, constructions of trails for abstract data types such as priority queues and union-find structures are given. These trails are applicable to any data-structure implementation of the abstract data type. It is also shown that these ideals lead naturally to monitors for data-structure operations.

  5. The Mormon Trail : historic byway inventory and evaluation

    DOT National Transportation Integrated Search

    1998-06-01

    The purpose of the Mormon Trail portion of the project was to research, field inventory and evaluate those roads that represent the Mormon's path across Iowa and identify the apparent and intrinsic qualities of these routes. Methods used to accomplis...

  6. Trail pheromone disruption of Argentine ant trail formation and foraging.

    PubMed

    Suckling, David Maxwell; Peck, Robert W; Stringer, Lloyd D; Snook, Kirsten; Banko, Paul C

    2010-01-01

    Trail pheromone disruption of invasive ants is a novel tactic that builds on the development of pheromone-based pest management in other insects. Argentine ant trail pheromone, (Z)-9-hexadecenal, was formulated as a micro-encapsulated sprayable particle and applied against Argentine ant populations in 400 m2 field plots in Hawai'i Volcanoes National Park. A widely dispersed point source strategy for trail pheromone disruption was used. Traffic rates of ants in bioassays of treated filter paper, protected from rainfall and sunlight, indicated the presence of behaviorally significant quantities of pheromone being released from the formulation for up to 59 days. The proportion of plots, under trade wind conditions (2–3 m s−1), with visible trails was reduced for up to 14 days following treatment, and the number of foraging ants at randomly placed tuna-bait cards was similarly reduced. The success of these trail pheromone disruption trials in a natural ecosystem highlights the potential of this method for control of invasive ant species in this and other environments.

  7. Trail Pheromone Disruption of Argentine Ant Trail Formation and Foraging

    USGS Publications Warehouse

    Suckling, D.M.; Peck, R.W.; Stringer, L.D.; Snook, K.; Banko, P.C.

    2010-01-01

    Trail pheromone disruption of invasive ants is a novel tactic that builds on the development of pheromone-based pest management in other insects. Argentine ant trail pheromone, (Z)-9-hexadecenal, was formulated as a micro-encapsulated sprayable particle and applied against Argentine ant populations in 400 m2 field plots in Hawai'i Volcanoes National Park. A widely dispersed point source strategy for trail pheromone disruption was used. Traffic rates of ants in bioassays of treated filter paper, protected from rainfall and sunlight, indicated the presence of behaviorally significant quantities of pheromone being released from the formulation for up to 59 days. The proportion of plots, under trade wind conditions (2-3 m s-1), with visible trails was reduced for up to 14 days following treatment, and the number of foraging ants at randomly placed tuna-bait cards was similarly reduced. The success of these trail pheromone disruption trials in a natural ecosystem highlights the potential of this method for control of invasive ant species in this and other environments. ?? Springer Science+Business Media, LLC 2010.

  8. Systemic immunological tolerance to ocular antigens is mediated by TNF-related apoptosis-inducing ligand (TRAIL)-expressing CD8+ T cells*

    PubMed Central

    Griffith, Thomas S.; Brincks, Erik L.; Gurung, Prajwal; Kucaba, Tamara A.; Ferguson, Thomas A.

    2010-01-01

    Systemic immunological tolerance to Ag encountered in the eye restricts the formation of potentially damaging immune responses that would otherwise be initiated at other anatomical locations. We previously demonstrated that tolerance to Ag administered via the anterior chamber (AC) of the eye required FasL-mediated apoptotic death of inflammatory cells that enter the eye in response to the antigenic challenge. Moreover, the systemic tolerance induced after AC injection of Ag was mediated by CD8+ regulatory T cells. The present study examined the mechanism by which these CD8+ regulatory T cells mediate tolerance after AC injection of Ag. AC injection of Ag did not prime CD4+ T cells, and led to increased TRAIL expression by splenic CD8+ T cells. Unlike wildtype mice, Trail−/− or Dr5−/− mice did not develop tolerance to Ag injected into the eye, even though responding lymphocytes underwent apoptosis in the AC of the eyes of these mice. CD8+ T cells from Trail−/− mice that were first injected AC with Ag were unable to transfer tolerance to naïve recipient wildtype mice, but CD8+ T cells from AC-injected wildtype or Dr5−/− mice could transfer tolerance. Importantly, the transferred wildtype (Trail+/+) CD8+ T cells were also able to decrease the number of infiltrating inflammatory cells into the eye; however, Trail−/− CD8+ T cells were unable to limit the inflammatory cell ingress. Together, our data suggest that “helpless” CD8+ regulatory T cells generated after AC injection of Ag enforce systemic tolerance in a TRAIL-dependent manner to inhibit inflammation in the eye. PMID:21169546

  9. Happy trails: the effect of a media campaign on urban trail use in southern Nevada.

    PubMed

    Clark, Sheila; Bungum, Tim J; Meacham, Mindy; Coker, Lisa

    2015-01-01

    Many Americans do not meet recommendations for physical activity (PA). Communities are building trail networks to encourage PA, but the relationship between trails and PA is not well understood. We monitored usage of urban trails (N = 10) in Las Vegas, NV, before and after a promotional marketing campaign (October 2011 and April 2012). The media campaign featured print, online, and radio ads, as well as billboards and signage on gas pumps. Data were collected with infrared monitors that were placed on the trails for periods of 7 days. We compared preintervention and postintervention usage rates. Mean usage increased (P < .001) from 3.91 to 5.95 users per hour (52.17%) after the promotional campaign. We observed significant increases at 7 individual trails, significant declines at 2 trails, and no change at 1 trail. Promotional campaigns may be an effective way to increase trail usage and encourage PA.

  10. Association between thyroid hormones and TRAIL.

    PubMed

    Bernardi, Stella; Bossi, Fleur; Toffoli, Barbara; Giudici, Fabiola; Bramante, Alessandra; Furlanis, Giulia; Stenner, Elisabetta; Secchiero, Paola; Zauli, Giorgio; Carretta, Renzo; Fabris, Bruno

    2017-11-01

    Recent studies suggest that a circulating protein called TRAIL (TNF-related apoptosis-inducing ligand) might have a role in the regulation of body weight and metabolism. Interestingly, thyroid hormones seem to increase TRAIL tissue expression. This study aimed at evaluating whether overt thyroid disorders affected circulating TRAIL levels. TRAIL circulating levels were measured in euthyroid, hyperthyroid, and hypothyroid patients before and after thyroid function normalization. Univariate and multivariate analyses were performed to evaluate the correlation between thyroid hormones and TRAIL. Then, the stimulatory effect of both triiodothyronine (T3) and thyroxine (T4) on TRAIL was evaluated in vitro on peripheral blood mononuclear cells. Circulating levels of TRAIL significantly increased in hyperthyroid and decreased in hypothyroid patients as compared to controls. Once thyroid function was restored, TRAIL levels normalized. There was an independent association between TRAIL and both fT3 and fT4. Consistent with these findings, T3 and T4 stimulated TRAIL release in vitro. Here we show that thyroid hormones are associated with TRAIL expression in vivo and stimulate TRAIL expression in vitro. Given the overlap between the metabolic effects of thyroid hormones and TRAIL, this work sheds light on the possibility that TRAIL might be one of the molecules mediating thyroid hormones peripheral effects. Copyright © 2017 The Authors. Published by Elsevier Inc. All rights reserved.

  11. Brownian trail rectified

    NASA Astrophysics Data System (ADS)

    Hurd, Alan J.; Ho, Pauline

    The experiments described here indicate when one of Nature's best fractals -- the Brownian trail -- becomes nonfractal. In most ambient fluids, the trail of a Brownian particle is self-similar over many decades of length. For example, the trail of a submicron particle suspended in an ordinary liquid, recorded at equal time intervals, exhibits apparently discontinuous changes in velocity from macroscopic lengths down to molecular lengths: the trail is a random walk with no velocity memory from one step to the next. In ideal Brownian motion, the kinks in the trail persist to infinitesimal time intervals, i.e., it is a curve without tangents. Even in real Brownian motion in a liquid, the time interval must be shortened to approximately 10(-8) s before the velocity appears continuous. In sufficiently rarefied environments, this time resolution at which a Brownian trail is rectified from a curve without tangents to a smoothly varying trajectory is greatly lengthened, making it possible to study the kinetic regime by dynamic light scattering. Our recent experiments with particles in a plasma have demonstrated this capability. In this regime, the particle velocity persists over a finite step length allowing an analogy to an ideal gas with Maxwell-Boltzmann velocities; the particle mass could be obtained from equipartition. The crossover from ballistic flight to hydrodynamic diffusion was also seen.

  12. 75 FR 12254 - Official Trail Marker for the Ala Kahakai National Historic Trail

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-15

    ... DEPARTMENT OF THE INTERIOR National Park Service Official Trail Marker for the Ala Kahakai National Historic Trail AGENCY: National Park Service, Interior. ACTION: Official Insignia, Designation. Authority: National Trails System Act, 16 U.S.C. 1241(a) and l246(c) and Protection of Official Badges...

  13. The Pimlico Chemistry Trail.

    ERIC Educational Resources Information Center

    Borrows, Peter

    1984-01-01

    Describes a chemistry "trail" (similar to a nature trail) which focuses on chemical phenomena in the environment. The trail includes 20 stops in and around a local school. Types of phenomena examined include building materials, air pollution, corrosion of metals, swimming pools, and others. Additional activities are also suggested. (DH)

  14. Prognostic value of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and TRAIL receptors in renal cell cancer.

    PubMed

    Macher-Goeppinger, Stephan; Aulmann, Sebastian; Tagscherer, Katrin E; Wagener, Nina; Haferkamp, Axel; Penzel, Roland; Brauckhoff, Antje; Hohenfellner, Markus; Sykora, Jaromir; Walczak, Henning; Teh, Bin T; Autschbach, Frank; Herpel, Esther; Schirmacher, Peter; Roth, Wilfried

    2009-01-15

    The death ligand tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and its receptors (TRAIL-R) are involved in immune surveillance and tumor development. Here, we studied a possible association between the expression of TRAIL/TRAIL-Rs and the prognosis in patients with renal cell carcinomas (RCC). A tissue microarray containing RCC tumor tissue samples and corresponding normal tissue samples from 838 patients was generated. Expression of TRAIL and TRAIL-Rs was examined by immunohistochemistry and the effect of TRAIL and TRAIL-R expression on disease-specific survival was assessed. High TRAIL-R2 expression levels were associated with high-grade RCCs (P < 0.001) and correlated negatively with disease-specific survival (P = 0.01). Similarly, high TRAIL expression was associated with a shorter disease-specific survival (P = 0.01). In contrast, low TRAIL-R4 expression was associated with high-stage RCCs (P < 0.001) as well as with the incidence of distant metastasis (P = 0.03) and correlated negatively with disease-specific survival (P = 0.02). In patients without distant metastasis, multivariate Cox regression analyses revealed that TRAIL-R2 and TRAIL are independent prognostic factors for cancer-specific survival (in addition to tumor extent, regional lymph node metastasis, grade of malignancy, and type of surgery). High TRAIL-R2, high TRAIL, and low TRAIL-R4 expression levels are associated with a worse disease-specific survival in patients with RCCs. Therefore, the assessment of TRAIL/TRAIL-R expression offers valuable prognostic information that could be used to select patients for adjuvant therapy studies. Moreover, our findings are of relevance for a potential experimental therapeutic administration of TRAIL-R agonists in patients with RCCs.

  15. Hikers and other trail users

    Treesearch

    Robert C. Lucas

    1971-01-01

    Trail users seem neglected. Trail systems are limited, largely relics of fire control rather than designed for recreation; and total trail miles are probably declining. On the other hand, participation in various kinds of trail-oriented recreation is substantial and growing. Most activity is for short periods of time close to participants' homes. A varied and...

  16. Compatibility of Ohio trail users

    Treesearch

    Roger E. McCay; George H. Moeller

    1976-01-01

    Compatibility indexes show how Ohio trail users feel about meeting each other on the trail. All four of the major types of trail users-hikers, horseback riders, bicycle riders, and motorcycle riders-enjoy meeting their own kind. But they also feel antagonism toward the faster, more mechanized trail users; e.g., everyone likes hikers, but few like motorcycle riders....

  17. National recreation trails: an overview

    Treesearch

    Joanne F. Tynon; James A. Harding; Deborah J. Chavez

    1998-01-01

    Since the establishment of the National Trails System in 1968, 822 trails have been designated as National Recreation Trails (NRT). These trails support a wide range of activities, including but not limited to hiking, mountain biking, cross country skiing and horseback riding. In short, NRTs can be found across a diversity of geographic settings and are frequent...

  18. Trails and physical activity: a review.

    PubMed

    Starnes, Heather A; Troped, Philip J; Klenosky, David B; Doehring, Angela M

    2011-11-01

    To provide a synthesis of research on trails and physical activity from the public health, leisure sciences, urban planning, and transportation literatures. A search of databases was conducted to identify studies published between 1980 and 2008. 52 studies were identified. The majority were cross-sectional (92%) and published after 1999 (77%). The evidence for the effects of trails on physical activity was mixed among 3 intervention and 5 correlational studies. Correlates of trail use were examined in 13 studies. Several demographic (eg, race, education, income) and environmental factors (eg, land-use mix and distance to trail) were related to trail use. Evidence from 31 descriptive studies identified several facilitators and barriers to trail use. Economic studies (n = 5) examining trails in terms of health or recreational outcomes found trails are cost-effective and produce significant economic benefits. There is a growing body of evidence demonstrating important factors that should be considered in promoting trail use, yet the evidence for positive effects of trails on physical activity is limited. Further research is needed to evaluate the effects of trails on physical activity. In addition, trail studies that include children and youth, older adults, and racial and ethnic minorities are a research priority.

  19. TRAIL-coated lipid-nanoparticles overcome resistance to soluble recombinant TRAIL in non-small cell lung cancer cells

    NASA Astrophysics Data System (ADS)

    De Miguel, Diego; Gallego-Lleyda, Ana; María Ayuso, José; Erviti-Ardanaz, Sandra; Pazo-Cid, Roberto; del Agua, Celia; José Fernández, Luis; Ochoa, Ignacio; Anel, Alberto; Martinez-Lostao, Luis

    2016-05-01

    Purpose. Non-small cell lung cancer (NSCLC) is one the types of cancer with higher prevalence and mortality. Apo2-Ligand/TRAIL is a TNF family member able to induce apoptosis in tumor cells but not in normal cells. It has been tested in clinical trials against different types of human cancer including NSCLC. However, results of clinical trials have shown a limited efficacy of TRAIL-based therapies. Recently we have demonstrated that artificial lipid nanoparticles coated with bioactive Apo2L/TRAIL (LUV-TRAIL) greatly improved TRAIL cytotoxic ability being capable of killing chemoresistant hematological cancer cells. In the present work we have extended the study to NSCLC. Methods/patients. LUV-TRAIL-induced cytotoxicity was assessed on different NSCLC cell lines with different sensitivity to soluble TRAIL and on primary human tumor cells from three patients suffering from NSCLC cancer. We also tested LUV-TRAIL-cytotoxic ability in combination with several anti-tumor agents. Results. LUV-TRAIL exhibited a greater cytotoxic effect compared to soluble TRAIL both in A549 cells and primary human NSCLC cells. LUV-TRAIL-induced cell death was dependent on caspase-8 and caspase-3 activation. Moreover, combination of LUV-TRAIL with other anti-tumor agents such as flavopiridol, and SNS-032 clearly enhanced LUV-TRAIL-induced cytotoxicity against NSCLC cancer cells. Conclusion. The novel formulation of TRAIL based on displaying it on the surface of lipid nanoparticles greatly increases its anti-tumor activity and has clinical potential in cancer treatment.

  20. TRAIL-coated lipid-nanoparticles overcome resistance to soluble recombinant TRAIL in non-small cell lung cancer cells.

    PubMed

    De Miguel, Diego; Gallego-Lleyda, Ana; Ayuso, José María; Erviti-Ardanaz, Sandra; Pazo-Cid, Roberto; del Agua, Celia; Fernández, Luis José; Ochoa, Ignacio; Anel, Alberto; Martinez-Lostao, Luis

    2016-05-06

    Non-small cell lung cancer (NSCLC) is one the types of cancer with higher prevalence and mortality. Apo2-Ligand/TRAIL is a TNF family member able to induce apoptosis in tumor cells but not in normal cells. It has been tested in clinical trials against different types of human cancer including NSCLC. However, results of clinical trials have shown a limited efficacy of TRAIL-based therapies. Recently we have demonstrated that artificial lipid nanoparticles coated with bioactive Apo2L/TRAIL (LUV-TRAIL) greatly improved TRAIL cytotoxic ability being capable of killing chemoresistant hematological cancer cells. In the present work we have extended the study to NSCLC. LUV-TRAIL-induced cytotoxicity was assessed on different NSCLC cell lines with different sensitivity to soluble TRAIL and on primary human tumor cells from three patients suffering from NSCLC cancer. We also tested LUV-TRAIL-cytotoxic ability in combination with several anti-tumor agents. LUV-TRAIL exhibited a greater cytotoxic effect compared to soluble TRAIL both in A549 cells and primary human NSCLC cells. LUV-TRAIL-induced cell death was dependent on caspase-8 and caspase-3 activation. Moreover, combination of LUV-TRAIL with other anti-tumor agents such as flavopiridol, and SNS-032 clearly enhanced LUV-TRAIL-induced cytotoxicity against NSCLC cancer cells. The novel formulation of TRAIL based on displaying it on the surface of lipid nanoparticles greatly increases its anti-tumor activity and has clinical potential in cancer treatment.

  1. Novel TRAIL sensitizer Taraxacum officinale F.H. Wigg enhances TRAIL-induced apoptosis in Huh7 cells.

    PubMed

    Yoon, Ji-Yong; Cho, Hyun-Soo; Lee, Jeong-Ju; Lee, Hyo-Jung; Jun, Soo Young; Lee, Jae-Hye; Song, Hyuk-Hwan; Choi, SangHo; Saloura, Vassiliki; Park, Choon Gil; Kim, Cheol-Hee; Kim, Nam-Soon

    2016-04-01

    TRAIL (TNF-related apoptosis inducing ligand) is a promising anti-cancer drug target that selectively induces apoptosis in cancer cells. However, many cancer cells are resistant to TRAIL-induced apoptosis. Therefore, reversing TRAIL resistance is an important step for the development of effective TRAIL-based anti-cancer therapies. We previously reported that knockdown of the TOR signaling pathway regulator-like (TIPRL) protein caused TRAIL-induced apoptosis by activation of the MKK7-c-Jun N-terminal Kinase (JNK) pathway through disruption of the MKK7-TIPRL interaction. Here, we identified Taraxacum officinale F.H. Wigg (TO) as a novel TRAIL sensitizer from a set of 500 natural products using an ELISA system and validated its activity by GST pull-down analysis. Furthermore, combination treatment of Huh7 cells with TRAIL and TO resulted in TRAIL-induced apoptosis mediated through inhibition of the MKK7-TIPRL interaction and subsequent activation of MKK7-JNK phosphorylation. Interestingly, HPLC analysis identified chicoric acid as a major component of the TO extract, and combination treatment with chicoric acid and TRAIL induced TRAIL-induced cell apoptosis via JNK activation due to inhibition of the MKK7-TIPRL interaction. Our results suggest that TO plays an important role in TRAIL-induced apoptosis, and further functional studies are warranted to confirm the importance of TO as a novel TRAIL sensitizer for cancer therapy. © 2015 Wiley Periodicals, Inc. © 2015 Wiley Periodicals, Inc.

  2. 75 FR 53735 - Notice of Final Federal Agency Actions on East Lake Sammamish Master Plan Trail in King County, WA

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-01

    ... on East Lake Sammamish Master Plan Trail in King County, WA AGENCY: Federal Highway Administration... enhancement project, the East Lake Sammamish Trail, starting at Gilman Boulevard in Issaquah, Washington and ending at Bear Creek Trail in Redmond, Washington. Those actions grant licenses, permits, and approvals...

  3. Trail impacts and trail impact management related to ecotourism visitation at Torres del Paine National Park, Chile

    USGS Publications Warehouse

    Farrell, T.A.; Marion, J.L.

    2002-01-01

    Ecotourism and protected area visitation in Central and South America are largely dependent upon a relatively undisturbed quality of natural resources. However, visitation may impact vegetation, soil, water and wildlife resources, and degrade visitor facilities such as recreation sites and trails. Findings are reported from trail impact research conducted at Torres del Paine National Park in Patagonia, Chile. The frequency and magnitude of selected trail impacts and the relative effect of the amount of use, vegetation type, trail position and trail grade are investigated. Findings differed from previous studies in that amount of use was significantly related to both trail width increases and trail erosion. Management actions to minimize trail impacts are offered.

  4. Low-Speed Fan Noise Reduction With Trailing Edge Blowing

    NASA Technical Reports Server (NTRS)

    Sutliff, Daniel L.; Tweedt, Daniel L.; Fite, E. Brian; Envia, Edmane

    2002-01-01

    An experimental proof-of-concept test was conducted to demonstrate reduction of rotor-stator interaction noise through rotor-trailing edge blowing. The velocity deficit from the viscous wake of the rotor blades was reduced by injecting air into the wake from a trailing edge slot. Composite hollow rotor blades with internal flow passages were designed based on analytical codes modeling the internal flow. The hollow blade with interior guide vanes creates flow channels through which externally supplied air flows from the root of the blade to the trailing edge. The impact of the rotor wake-stator interaction on the acoustics was also predicted analytically. The Active Noise Control Fan, located at the NASA Glenn Research Center, was used as the proof- of-concept test bed. In-duct mode and farfield directivity acoustic data were acquired at blowing rates (defined as mass supplied to trailing edge blowing system divided by fan mass flow) ranging from 0.5 to 2.0 percent. The first three blade passing frequency harmonics at fan rotational speeds of 1700 to 1900 rpm were analyzed. The acoustic tone power levels (PWL) in the inlet and exhaust were reduced 11.5 and -0.1, 7.2 and 11.4, 11.8 and 19.4 PWL dB, respectively. The farfield tone power levels at the first three harmonics were reduced 5.4, 10.6, and 12.4 dB PWL. At selected conditions, two-component hotwire and stator vane unsteady surface pressures were acquired. These measurements illustrate the physics behind the noise reduction.

  5. Modeling sediment transport from an off-road vehicle trail stream crossing using WEPP model

    Treesearch

    Renee' D. Ayala; Puneet Srivastava; Christian J. Brodbeck; Emily A. Carter; Timothy P. McDonald

    2005-01-01

    There is a limited information available pertaining to the adverse effects of Off-Road-Vehicle (ORV) use and trail impacts. As a result, this study was initiated in 2003 to (a) quantify water quality impacts of an ORV trail stream crossing through monitoring of total suspended solids, and (b) conduct WEPP (Water Erosion Prediction Project) simulations to determine long...

  6. 75 FR 37463 - Official Trail Marker for the Star-Spangled Banner National Historic Trail

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-29

    ... DEPARTMENT OF THE INTERIOR National Park Service Official Trail Marker for the Star-Spangled Banner National Historic Trail AGENCY: National Parks Service, Interior. ACTION: Official Insignia, Designation. Authority: National Trails System Act, 16 U.S.C. 124(a) and 1246(c) and Protection of Official...

  7. The Biology of TRAIL and the Role of TRAIL-Based Therapeutics in Infectious Diseases

    PubMed Central

    Shepard, Brett D.; Badley, Andrew D.

    2011-01-01

    TNF-related apoptosis inducing ligand (TRAIL) is a key mediator of the innate immune response to infection. While TRAIL-mediated apoptosis plays an essential role in the clearance of virus-infected cells, its physiologic role also includes immunosurveilance for cancer cells. Therapeutics that induce TRAIL-mediated apoptosis in cancer cells remain a focus of ongoing investigation in clinical trials, and much has been learned from these studies regarding the efficacy and toxicity of these interventions. These data, combined with data from numerous preclinical studies that detail the important and multifaceted role of TRAIL during infection with human immunodeficiency virus and other viruses, suggest that therapeutic exploitation of TRAIL signaling offers a novel and efficacious strategy for the management of infectious diseases. PMID:21857885

  8. Lipid Nanoparticles Decorated with TNF-Related Aptosis-Inducing Ligand (TRAIL) Are More Cytotoxic than Soluble Recombinant TRAIL in Sarcoma.

    PubMed

    Gallego-Lleyda, Ana; De Miguel, Diego; Anel, Alberto; Martinez-Lostao, Luis

    2018-05-13

    Sarcomas are rare and heterogeneous cancers classically associated with a poor outcome. Sarcomas are 1% of the cancer but recent estimations indicate that sarcomas account for 2% of the estimated cancer-related deaths. Traditional treatment with surgery, radiotherapy, and chemotherapy has improved the outcome for some types of sarcomas. However, novel therapeutic strategies to treat sarcomas are necessary. TNF-related apoptosis-inducing ligand (TRAIL) is a death ligand initially described as capable of inducing apoptosis on tumor cell while sparing normal cells. Only few clinical trials have used TRAIL-based treatments in sarcoma, but they show only low or moderate efficacy of TRAIL. Consequently, novel TRAIL formulations with an improved TRAIL bioactivity are necessary. Our group has developed a novel TRAIL formulation based on tethering this death ligand on a lipid nanoparticle surface (LUV-TRAIL) resembling the physiological secretion of TRAIL as a trasmembrane protein inserted into the membrane of exosomes. We have already demonstrated that LUV-TRAIL shows an improved cytotoxic activity when compared to soluble recombinant TRAIL both in hematological malignancies and epithelial-derived cancers. In the present study, we have tested LUV-TRAIL in several human sarcoma tumor cell lines with different sensitivity to soluble recombinant TRAIL, finding that LUV-TRAIL was more efficient than soluble recombinant TRAIL. Moreover, combined treatment of LUV-TRAIL with distinct drugs proved to be especially effective, sensitizing even more resistant cell lines to TRAIL.

  9. Global Variation of Meteor Trail Plasma Turbulence

    NASA Technical Reports Server (NTRS)

    Dyrud, L. P.; Hinrichs, J.; Urbina, J.

    2011-01-01

    We present the first global simulations on the occurrence of meteor trail plasma irregularities. These results seek to answer the following questions: when a meteoroid disintegrates in the atmosphere will the resulting trail become plasma turbulent, what are the factors influencing the development of turbulence, and how do they vary on a global scale. Understanding meteor trail plasma turbulence is important because turbulent meteor trails are visible as non-specular trails to coherent radars, and turbulence influences the evolution of specular radar meteor trails, particularly regarding the inference of mesospheric temperatures from trail diffusion rates, and their usage for meteor burst communication. We provide evidence of the significant effect that neutral atmospheric winds and density, and ionospheric plasma density have on the variability of meteor trail evolution and the observation of nonspecular meteor trails, and demonstrate that trails are far less likely to become and remain turbulent in daylight, explaining several observational trends using non-specular and specular meteor trails.

  10. Small Molecular TRAIL Inducer ONC201 Induces Death in Lung Cancer Cells: A Preclinical Study

    PubMed Central

    Feng, Yuan; Zhou, Jihong; Li, Zhanhua; Jiang, Ying; Zhou, Ying

    2016-01-01

    Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) selectively targets cancer cells. The present preclinical study investigated the anti-cancer efficiency of ONC201, a first-in-class small molecule TRAIL inducer, in lung cancer cells. We showed that ONC201 was cytotoxic and anti-proliferative in both established (A549 and H460 lines) and primary human lung cancer cells. It was yet non-cytotoxic to normal lung epithelial cells. Further, ONC201 induced exogenous apoptosis activation in lung cancer cells, which was evidenced by TRAIL/death receptor-5 (DR5) induction and caspase-8 activation. The caspase-8 inhibitor or TRAIL/DR5 siRNA knockdown alleviated ONC201’s cytotoxicity against lung cancer cells. Molecularly, ONC201 in-activated Akt-S6K1 and Erk signalings in lung cancer cells, causing Foxo3a nuclear translocation. For the in vivo studies, intraperitoneal injection of ONC201 at well-tolerated doses significantly inhibited xenografted A549 tumor growth in severe combined immunodeficient (SCID) mice. Further, ONC201 administration induced TRAIL/DR5 expression, yet inactivated Akt-S6K1 and Erk in tumor tissues. These results of the study demonstrates the potent anti-lung cancer activity by ONC201. PMID:27626799

  11. Small Molecular TRAIL Inducer ONC201 Induces Death in Lung Cancer Cells: A Preclinical Study.

    PubMed

    Feng, Yuan; Zhou, Jihong; Li, Zhanhua; Jiang, Ying; Zhou, Ying

    2016-01-01

    Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) selectively targets cancer cells. The present preclinical study investigated the anti-cancer efficiency of ONC201, a first-in-class small molecule TRAIL inducer, in lung cancer cells. We showed that ONC201 was cytotoxic and anti-proliferative in both established (A549 and H460 lines) and primary human lung cancer cells. It was yet non-cytotoxic to normal lung epithelial cells. Further, ONC201 induced exogenous apoptosis activation in lung cancer cells, which was evidenced by TRAIL/death receptor-5 (DR5) induction and caspase-8 activation. The caspase-8 inhibitor or TRAIL/DR5 siRNA knockdown alleviated ONC201's cytotoxicity against lung cancer cells. Molecularly, ONC201 in-activated Akt-S6K1 and Erk signalings in lung cancer cells, causing Foxo3a nuclear translocation. For the in vivo studies, intraperitoneal injection of ONC201 at well-tolerated doses significantly inhibited xenografted A549 tumor growth in severe combined immunodeficient (SCID) mice. Further, ONC201 administration induced TRAIL/DR5 expression, yet inactivated Akt-S6K1 and Erk in tumor tissues. These results of the study demonstrates the potent anti-lung cancer activity by ONC201.

  12. Systemic PEGylated TRAIL treatment ameliorates liver cirrhosis in rats by eliminating activated hepatic stellate cells.

    PubMed

    Oh, Yumin; Park, Ogyi; Swierczewska, Magdalena; Hamilton, James P; Park, Jong-Sung; Kim, Tae Hyung; Lim, Sung-Mook; Eom, Hana; Jo, Dong Gyu; Lee, Choong-Eun; Kechrid, Raouf; Mastorakos, Panagiotis; Zhang, Clark; Hahn, Sei Kwang; Jeon, Ok-Cheol; Byun, Youngro; Kim, Kwangmeyung; Hanes, Justin; Lee, Kang Choon; Pomper, Martin G; Gao, Bin; Lee, Seulki

    2016-07-01

    Liver fibrosis is a common outcome of chronic liver disease that leads to liver cirrhosis and hepatocellular carcinoma. No US Food and Drug Administration-approved targeted antifibrotic therapy exists. Activated hepatic stellate cells (aHSCs) are the major cell types responsible for liver fibrosis; therefore, eradication of aHSCs, while preserving quiescent HSCs and other normal cells, is a logical strategy to stop and/or reverse liver fibrogenesis/fibrosis. However, there are no effective approaches to specifically deplete aHSCs during fibrosis without systemic toxicity. aHSCs are associated with elevated expression of death receptors and become sensitive to tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced cell death. Treatment with recombinant TRAIL could be a potential strategy to ameliorate liver fibrosis; however, the therapeutic application of recombinant TRAIL is halted due to its very short half-life. To overcome this problem, we previously generated PEGylated TRAIL (TRAILPEG ) that has a much longer half-life in rodents than native-type TRAIL. In this study, we demonstrate that intravenous TRAILPEG has a markedly extended half-life over native-type TRAIL in nonhuman primates and has no toxicity in primary human hepatocytes. Intravenous injection of TRAILPEG directly induces apoptosis of aHSCs in vivo and ameliorates carbon tetrachloride-induced fibrosis/cirrhosis in rats by simultaneously down-regulating multiple key fibrotic markers that are associated with aHSCs. TRAIL-based therapies could serve as new therapeutics for liver fibrosis/cirrhosis and possibly other fibrotic diseases. (Hepatology 2016;64:209-223). © 2015 by the American Association for the Study of Liver Diseases.

  13. Inhibition of TRAIL-induced apoptosis and forced internalization of TRAIL receptor 1 by adenovirus proteins.

    PubMed

    Tollefson, A E; Toth, K; Doronin, K; Kuppuswamy, M; Doronina, O A; Lichtenstein, D L; Hermiston, T W; Smith, C A; Wold, W S

    2001-10-01

    Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) induces apoptosis through two receptors, TRAIL-R1 (also known as death receptor 4) and TRAIL-R2 (also known as death receptor 5), that are members of the TNF receptor superfamily of death domain-containing receptors. We show that human adenovirus type 5 encodes three proteins, named RID (previously named E3-10.4K/14.5K), E3-14.7K, and E1B-19K, that independently inhibit TRAIL-induced apoptosis of infected human cells. This conclusion was derived from studies using wild-type adenovirus, adenovirus replication-competent mutants that lack one or more of the RID, E3-14.7K, and E1B-19K genes, and adenovirus E1-minus replication-defective vectors that express all E3 genes, RID plus E3-14.7K only, RID only, or E3-14.7K only. RID inhibits TRAIL-induced apoptosis when cells are sensitized to TRAIL either by adenovirus infection or treatment with cycloheximide. RID induces the internalization of TRAIL-R1 from the cell surface, as shown by flow cytometry and indirect immunofluorescence for TRAIL-R1. TRAIL-R1 was internalized in distinct vesicles which are very likely to be endosomes and lysosomes. TRAIL-R1 is degraded, as indicated by the disappearance of the TRAIL-R1 immunofluorescence signal. Degradation was inhibited by bafilomycin A1, a drug that prevents acidification of vesicles and the sorting of receptors from late endosomes to lysosomes, implying that degradation occurs in lysosomes. RID was also shown previously to internalize and degrade another death domain receptor, Fas, and to prevent apoptosis through Fas and the TNF receptor. RID was shown previously to force the internalization and degradation of the epidermal growth factor receptor. E1B-19K was shown previously to block apoptosis through Fas, and both E1B-19K and E3-14.7K were found to prevent apoptosis through the TNF receptor. These findings suggest that the receptors for TRAIL, Fas ligand, and TNF play a role in limiting virus

  14. Inhibition of TRAIL-Induced Apoptosis and Forced Internalization of TRAIL Receptor 1 by Adenovirus Proteins

    PubMed Central

    Tollefson, Ann E.; Toth, Karoly; Doronin, Konstantin; Kuppuswamy, Mohan; Doronina, Oksana A.; Lichtenstein, Drew L.; Hermiston, Terry W.; Smith, Craig A.; Wold, William S. M.

    2001-01-01

    Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) induces apoptosis through two receptors, TRAIL-R1 (also known as death receptor 4) and TRAIL-R2 (also known as death receptor 5), that are members of the TNF receptor superfamily of death domain-containing receptors. We show that human adenovirus type 5 encodes three proteins, named RID (previously named E3-10.4K/14.5K), E3-14.7K, and E1B-19K, that independently inhibit TRAIL-induced apoptosis of infected human cells. This conclusion was derived from studies using wild-type adenovirus, adenovirus replication-competent mutants that lack one or more of the RID, E3-14.7K, and E1B-19K genes, and adenovirus E1-minus replication-defective vectors that express all E3 genes, RID plus E3-14.7K only, RID only, or E3-14.7K only. RID inhibits TRAIL-induced apoptosis when cells are sensitized to TRAIL either by adenovirus infection or treatment with cycloheximide. RID induces the internalization of TRAIL-R1 from the cell surface, as shown by flow cytometry and indirect immunofluorescence for TRAIL-R1. TRAIL-R1 was internalized in distinct vesicles which are very likely to be endosomes and lysosomes. TRAIL-R1 is degraded, as indicated by the disappearance of the TRAIL-R1 immunofluorescence signal. Degradation was inhibited by bafilomycin A1, a drug that prevents acidification of vesicles and the sorting of receptors from late endosomes to lysosomes, implying that degradation occurs in lysosomes. RID was also shown previously to internalize and degrade another death domain receptor, Fas, and to prevent apoptosis through Fas and the TNF receptor. RID was shown previously to force the internalization and degradation of the epidermal growth factor receptor. E1B-19K was shown previously to block apoptosis through Fas, and both E1B-19K and E3-14.7K were found to prevent apoptosis through the TNF receptor. These findings suggest that the receptors for TRAIL, Fas ligand, and TNF play a role in limiting virus

  15. 75 FR 37462 - Official Trail Marker for the Captain John Smith Chesapeake National Historic Trail

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-29

    ... DEPARTMENT OF THE INTERIOR National Park Service Official Trail Marker for the Captain John Smith Chesapeake National Historic Trail AGENCY: National Parks Service, Interior. ACTION: Official Insignia, Designation. Authority: National Trails System Act, 16 U.S.C. 124(a) and 1246(c) and Protection of Official...

  16. Trail deterioration as an indicator of trail use in an urban forest recreation area

    Treesearch

    Thomas A. More

    1980-01-01

    The average width of a trail was used to predict trail use in an urban forest recreation area. Results show that width indicates use only very generally at best. Consequently, simply inspecting the physical condition of a trail may lead to erroneous conclusions about its use. Managers requiring more than a simple light use/heavy use classification should adopt more...

  17. Awareness and use of community walking trails.

    PubMed

    Reed, Julian A; Ainsworth, Barbara E; Wilson, Dawn K; Mixon, Gary; Cook, Annette

    2004-11-01

    Community trail development is an emerging strategy to increase physical activity (PA) among community residents. The purpose of this study was to assess awareness and use of trails and compare perceptions to objective data. A telephone survey was administered to a stratified sample of adults (N = 1,112) in a southeastern county in the United States. Respondents' home addresses and the locations of trails were entered into a GIS database. A kappa statistic was used to measure agreement between awareness and presence of trails. Differences in reported trail use patterns by sex, race, education, and PA levels were evaluated. There was no agreement between the awareness and presence of trails (kappa = 0.07). Fifty-six percent of the respondents reported having trails; however, only 33% reported using the trails. Of the trail users, 42% reported being regularly active in moderate-to-vigorous PA (30+ min/day for 5+ days/week), and 51% reported being less active (P < 0.003). Among walkers (> or =30 min/day for > or =5 days/week), 49% of regular walkers and 35% of irregular walkers (< walkers) reported using the trails (P < 0.04). Awareness of existing trails in this community and levels of use were low. Marketing programs should promote awareness and use of trails among older adults and irregularly active adults.

  18. High-order TRAIL oligomer formation in TRAIL-coated lipid nanoparticles enhances DR5 cross-linking and increases antitumour effect against colon cancer.

    PubMed

    De Miguel, Diego; Gallego-Lleyda, Ana; Ayuso, José María; Pejenaute-Ochoa, Dolores; Jarauta, Vidal; Marzo, Isabel; Fernández, Luis J; Ochoa, Ignacio; Conde, Blanca; Anel, Alberto; Martinez-Lostao, Luis

    2016-12-28

    During the last years, a great effort has been invested into developing new TRAIL formulations with increased bioactivity, trying to overcome the resistance to conventional soluble TRAIL (sTRAIL) exhibited by many primary tumours. In our group, we have generated artificial lipid nanoparticles decorated with sTRAIL (LUV-TRAIL), emulating the physiological TRAIL-containing exosomes by which T-cells release TRAIL upon activation. We already demonstrated that LUV-TRAIL has greater cytotoxicity against both chemoresistant haematologic tumour cells and epithelial carcinoma cells compared to a form of sTRAIL similar to that used in clinical trials. In this study we have tested LUV-TRAIL in several human colon cancer cell lines with different sensitivity to sTRAIL. LUV-TRAIL significantly improved sTRAIL cytotoxicity in all colon cancer cell lines tested. Trying to ascertain the molecular mechanism by which LUV-TRAIL exhibited improved cytotoxicity, we demonstrated that TRAIL-coated lipid nanoparticles were able to activate DR5 more efficiently than sTRAIL, and this relied on LUV-TRAIL ability to promote DR5 clustering on the cell surface. Moreover, we show that TRAIL molecules are arranged in higher order oligomers only in LUV-TRAIL, which may explain their enhanced DR5 clustering ability. Finally, LUV-TRAIL showed significantly better antitumour activity than sTRAIL in an in vivo model using HCT-116 xenograft tumours in nude mice, validating its potential clinical application. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

  19. Active and Public Transportation Connectivity between North Temple TOD and Jordan Park River Trail

    DOT National Transportation Integrated Search

    2017-10-01

    The project seeks to capitalize on existing community assets-several TOD stations and a regional bike and pedestrian trail system-by studying how these can be linked. The overarching goal of this project is to increase scholarship on networking safe ...

  20. Comparing impacts between formal and informal recreational trails.

    PubMed

    Pickering, Catherine Marina; Norman, Patrick

    2017-05-15

    Globally there are hundreds of thousands of kilometres of recreational trails traversing natural areas of high conservation value: but what are their impacts and do impacts differ among trails? We compared the effects of four common types of recreational trails [(1) narrow and (2) medium width informal bare earth trails and (3) gravel and (4) tarmac/concrete formal trails] on vegetation adjacent to trails in a high conservation value plant community that is popular for mountain biking and hiking in Australia. Plant species composition was recorded in quadrats along the edge of the four types of trails and in control sites away from trails. Vegetation cover, the cover of individual growth forms, and species richness along the edges of all four types of trails were similar to the controls, although the wider trails affected plant composition, with the tarmac and gravel trails favouring different species. With very few comparative studies, more research is required to allow managers and researchers to directly compare differences in the severity and types of impacts on vegetation among trails. In the meantime, limiting damage to vegetation on the edge of hardened trails during construction, use and maintenance is important, and hardening trails may not always be appropriate. Copyright © 2016. Published by Elsevier Ltd.

  1. Fasting Enhances TRAIL-Mediated Liver Natural Killer Cell Activity via HSP70 Upregulation

    PubMed Central

    Dang, Vu T. A.; Tanabe, Kazuaki; Tanaka, Yuka; Tokumoto, Noriaki; Misumi, Toshihiro; Saeki, Yoshihiro; Fujikuni, Nobuaki; Ohdan, Hideki

    2014-01-01

    Acute starvation, which is frequently observed in clinical practice, sometimes augments the cytolytic activity of natural killer cells against neoplastic cells. In this study, we investigated the molecular mechanisms underlying the enhancement of natural killer cell function by fasting in mice. The total number of liver resident natural killer cells in a unit weight of liver tissue obtained from C57BL/6J mice did not change after a 3-day fast, while the proportions of tumor necrosis factor–related apoptosis-inducing ligand (TRAIL)+ and CD69+ natural killer cells were significantly elevated (n = 7, p <0.01), as determined by flow cytometric analysis. Furthermore, we found that TRAIL− natural killer cells that were adoptively transferred into Rag-2−/− γ chain−/− mice could convert into TRAIL+ natural killer cells in fasted mice at a higher proportion than in fed mice. Liver natural killer cells also showed high TRAIL-mediated antitumor function in response to 3-day fasting. Since these fasted mice highly expressed heat shock protein 70 (n = 7, p <0.05) in liver tissues, as determined by western blot, the role of this protein in natural killer cell activation was investigated. Treatment of liver lymphocytes with 50 µg/mL of recombinant heat shock protein 70 led to the upregulation of both TRAIL and CD69 in liver natural killer cells (n = 6, p <0.05). In addition, HSP70 neutralization by intraperitoneally injecting an anti- heat shock protein 70 monoclonal antibody into mice prior to fasting led to the downregulation of TRAIL expression (n = 6, p <0.05). These findings indicate that acute fasting enhances TRAIL-mediated liver natural killer cell activity against neoplastic cells through upregulation of heat shock protein 70. PMID:25356750

  2. SAHM:VisTrails (Software for Assisted Habitat Modeling for VisTrails): training course

    USGS Publications Warehouse

    Holcombe, Tracy

    2014-01-01

    VisTrails is an open-source management and scientific workflow system designed to integrate the best of both scientific workflow and scientific visualization systems. Developers can extend the functionality of the VisTrails system by creating custom modules for bundled VisTrails packages. The Invasive Species Science Branch of the U.S. Geological Survey (USGS) Fort Collins Science Center (FORT) and the U.S. Department of the Interior’s North Central Climate Science Center have teamed up to develop and implement such a module—the Software for Assisted Habitat Modeling (SAHM). SAHM expedites habitat modeling and helps maintain a record of the various input data, the steps before and after processing, and the modeling options incorporated in the construction of an ecological response model. There are four main advantages to using the SAHM:VisTrails combined package for species distribution modeling: (1) formalization and tractable recording of the entire modeling process; (2) easier collaboration through a common modeling framework; (3) a user-friendly graphical interface to manage file input, model runs, and output; and (4) extensibility to incorporate future and additional modeling routines and tools. In order to meet increased interest in the SAHM:VisTrails package, the FORT offers a training course twice a year. The course includes a combination of lecture, hands-on work, and discussion. Please join us and other ecological modelers to learn the capabilities of the SAHM:VisTrails package.

  3. 3-Bromopyruvate enhances TRAIL-induced apoptosis in human nasopharyngeal carcinoma cells through CHOP-dependent upregulation of TRAIL-R2.

    PubMed

    Can, Zhou; Lele, Song; Zhirui, Zhang; Qiong, Pan; Yuzhong, Chen; Lingling, Liu; Surong, Zhao; Yiming, Sun; Pei, Zhang; Chenchen, Jiang; Liu, Hao

    2017-08-01

    Past reports have shown that the sensitivity of cancer cells to TRAIL-induced apoptosis is related to their expression of TRAIL-death receptors on the cell surface. However, the level of TRAIL-death receptors expression on cancer cells is always low. Our previous research showed that nasopharyngeal carcinoma (NPC) cells have a poor sensitivity to low doses of TRAIL. Here, we evaluated combined treatment with the energy inhibitor 3-bromopyruvate (3BP) and TRAIL as a method to produce an increased apoptotic response in NPC cells. The results showed that 3BP and TRAIL together produced higher cytotoxicity and increased TRAIL-R2 expression in NPC cells compared with the effects of either 3BP or TRAIL alone. These findings led us to hypothesize that 3BP may sensitize NPC cells to TRAIL. 3BP is a metabolic blocker that inhibits hexokinase II activity, suppresses ATP production, and induces endoplasmic reticulum (ER) stress. Our results showed that 3BP also activated AMP-activated protein kinase, which we found to play an important role in the induction of ER stress by 3BP. Furthermore, the induction of TRAIL-R2 expression and the sensitization of the NPC cells to TRAIL by 3BP were reduced when we inhibited the expression of CHOP. Taken together, our results showed that a low dose of 3BP sensitized NPC cells to TRAIL-induced apoptosis by the upregulation of CHOP, which was mediated by the activation of AMP-activated protein kinase and ER stress. The results showed that 3BP is a promising candidate agent for enhancing the therapeutic response to TRAIL in NPC.

  4. Environmental perceptions and objective walking trail audits inform a community-based participatory research walking intervention

    PubMed Central

    2012-01-01

    Background Given the documented physical activity disparities that exist among low-income minority communities and the increased focused on socio-ecological approaches to address physical inactivity, efforts aimed at understanding the built environment to support physical activity are needed. This community-based participatory research (CBPR) project investigates walking trails perceptions in a high minority southern community and objectively examines walking trails. The primary aim is to explore if perceived and objective audit variables predict meeting recommendations for walking and physical activity, MET/minutes/week of physical activity, and frequency of trail use. Methods A proportional sampling plan was used to survey community residents in this cross-sectional study. Previously validated instruments were pilot tested and appropriately adapted and included the short version of the validated International Physical Activity Questionnaire, trail use, and perceptions of walking trails. Walking trails were assessed using the valid and reliable Path Environmental Audit Tool which assesses four content areas including: design features, amenities, maintenance, and pedestrian safety from traffic. Analyses included Chi-square, one-way ANOVA's, multiple linear regression, and multiple logistic models. Results Numerous (n = 21) high quality walking trails were available. Across trails, there were very few indicators of incivilities and safety features rated relatively high. Among the 372 respondents, trail use significantly predicted meeting recommendations for walking and physical activity, and MET/minutes/week. While controlling for other variables, significant predictors of trail use included proximity to trails, as well as perceptions of walking trail safety, trail amenities, and neighborhood pedestrian safety. Furthermore, while controlling for education, gender, and income; for every one time per week increase in using walking trails, the odds for meeting walking

  5. Trail-following responses of the Argentine ant,Iridomyrmex humilis (Mayr), to a synthetic trail pheromone component and analogs.

    PubMed

    Van Vorhis Key, S E; Baker, T C

    1982-01-01

    Behavioral evidence indicates that (Z)-9-hexadecenal (Z9-16∶ALD) is a trail pheromone component ofIridomyrmex humilis, and that the true trail pheromone may be multicomponent. Trail-following responses ofI. humilis workers to several concentrations of syntheticZ9-16∶ALD, a constituent of the Pavan's gland, were found to be comparable to responses to gaster extract trails containing ca. 100 times lessZ9-16∶ALD. Of the five aldehyde analogs tested, only (Z)-7-hexadecenal (Z7-16∶ALD) elicited significant trail-following. However, following responses to severalZ9-16∶ALD-Z7-16∶ALD combinations were lower than responses toZ9-16∶ALD alone. Trails on filter paper of biologically relevant concentrations ofZ9-16∶ALD lose activity within 2 hr in the laboratory. The release rate ofZ9-16∶ALD measured from filter paper trails was 0.25 ± 0.10 pg/cm-sec. This was used to estimate the trail-following threshold for this compound of Argentine ant workers.

  6. Broadband Noise Reduction of a Low-Speed Fan Noise Using Trailing Edge Blowing

    NASA Technical Reports Server (NTRS)

    Sutliff, Daniel L.

    2005-01-01

    An experimental proof-of-concept test was conducted to demonstrate reduction of rotor-stator interaction noise through the use of rotor-trailing edge blowing. The velocity deficit from the viscous wake of the rotor blades was reduced by injecting air into the wake from a continuous trailing edge slot. Hollow blades with interior guide vanes create flow channels through which externally supplied air flows from the blade root to the trailing edge. A previous paper documented the substantial tonal reductions of this Trailing Edge Rotor Blowing (TERB) fan. This report documents the broadband characteristics of TERB. The Active Noise Control Fan (ANCF), located at the NASA Glenn Research Center, was used as the proof-of-concept test bed. Two-component hotwire data behind the rotor, unsteady surface pressures on the stator vane, and farfield directivity acoustic data were acquired at blowing rates of 1.1, 1.5, and 1.8 percent of the total fan mass flow. The results indicate a substantial reduction in the rotor wake turbulent velocity and in the stator vane unsteady surface pressures. Based on the physics of the noise generation, these indirect measurements indicate the prospect of broadband noise reduction. However, since the broadband noise generated by the ANCF is rotor-dominated, any change in the rotor-stator interaction broadband noise levels is barely distinguishable in the farfield measurements.

  7. Estimating the economic value and impacts of recreational trails: a case study of the Virginia creeper rail trail

    Treesearch

    J. Michael Bowker; John C. Bergstrom; Joshua Gill

    2007-01-01

    Many communities are interested in developing and maintaining recreational trails to benefit trail users and as tourist attractions to stimulate economic growth. In this paper, a study is described which estimates the net economic value to trail users and the local economic impacts of the Virginia Creeper Rail Trail in south-western Virginia, USA. The monetary...

  8. 30 CFR 75.602 - Trailing cable junctions.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Trailing cable junctions. 75.602 Section 75.602... MANDATORY SAFETY STANDARDS-UNDERGROUND COAL MINES Trailing Cables § 75.602 Trailing cable junctions. [Statutory Provision] When two or more trailing cables junction to the same distribution center, means shall...

  9. 30 CFR 75.828 - Trailing cable pulling.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Trailing cable pulling. 75.828 Section 75.828... Longwalls § 75.828 Trailing cable pulling. The trailing cable must be de-energized prior to being pulled by... procedures must be followed when pulling the trailing cable with equipment other than the continuous mining...

  10. A novel gene from the takeout family involved in termite trail-following behavior.

    PubMed

    Schwinghammer, Margaret A; Zhou, Xuguo; Kambhampati, Srinivas; Bennett, Gary W; Scharf, Michael E

    2011-03-15

    This study investigated physiological and behavioral functions of a novel gene identified from the termite Reticulitermes flavipes. The gene, named deviate, encodes an apparent ligand binding protein from the takeout-homologous family. Initial studies were conducted to investigate deviate mRNA expression among termite castes and body regions, and changes in response to light-dark conditions, starvation, temperature, and juvenile hormone (JH). Deviate has ubiquitous caste and tissue expression, including antennal expression. Consistent with characteristics of other takeout family members, deviate expression is responsive to photophase conditions (p<0.1), and feeding, temperature, and JH (p<0.05). Using RNA-interference (RNAi) techniques, short-interfering RNAs (siRNAs) homologous to the deviate gene were synthesized and injected into worker termites, which were then subjected to bioassays designed to (1) induce caste differentiation or (2) measure various behavioral aspects of foraging and trail following. No impacts on JH-dependent caste differentiation were observable. However, trail following accuracy was significantly reduced in termites that received deviate siRNA injections, and this pattern generally mirrored deviate mRNA attenuation and recovery after RNAi. In a subsequent distance foraging bioassay, deviate-silenced termites exhibited equal feeding levels to controls, suggesting the deviate gene is not linked to general vigor or the ability/motivation of termites to move and forage. These findings are among the first linking the expression of a termite gene with eusocial behavior; they illustrate the connection between deviate expression and trailing behavior, which is a key evolutionary adaptation vital to subterranean social insects such as termites and ants. Copyright © 2010 Elsevier B.V. All rights reserved.

  11. 36 CFR 13.1308 - Harding Icefield Trail.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 36 Parks, Forests, and Public Property 1 2010-07-01 2010-07-01 false Harding Icefield Trail. 13... Provisions § 13.1308 Harding Icefield Trail. The Harding Icefield Trail from the junction with the main paved trail near Exit Glacier to the emergency hut near the terminus is closed to— (a) Camping within 1/8 mile...

  12. Trails research: where do we go from here?

    Treesearch

    Michael A. Schuett; Patricia Seiser

    2002-01-01

    This paper describes a recent study focusing on trails research needs. This study was supported by American Trails. Using a Delphi technique, 86 trails experts representing a variety of federal, state and local agencies, nonprofits, and trail uses were queried by email on trails research needs. A Delphi technique is a prognostic tool for dealing with complex problems...

  13. Miles In Trail (MIT) Restrictions: A Perspective

    NASA Technical Reports Server (NTRS)

    Kopardekar, Parimal; Green, Steven; Roherty, Tom; Aston, John

    2003-01-01

    Miles-in-trail restrictions are issued to meet the airport and/or airspace capacity. The purpose of this paper is to review the currently practiced miles-in-trail operations for traffic flow management at a typical en route Air Traffic Control Center. The paper describes roles and considerations of both traffic management coordinators and the controllers in planning, coordination, execution, and monitoring of miles-in-trail restrictions. The paper addresses the type of decisions that traffic management coordinators must make and the different information required to plan and monitor miles-in-trail restrictions. The implications of miles-in-trail restrictions on controller workload are also addressed. Using the Cleveland center as an example, the paper also identified some challenging traffic situations that required miles-in-trail restrictions on a regular basis. The paper is expected to benefit the research and development community as it provides the current challenges in traffic flow management and strengths and weakness of miles-in-trail operations.

  14. Aggregation-fragmentation-diffusion model for trail dynamics

    DOE PAGES

    Kawagoe, Kyle; Huber, Greg; Pradas, Marc; ...

    2017-07-21

    We investigate statistical properties of trails formed by a random process incorporating aggregation, fragmentation, and diffusion. In this stochastic process, which takes place in one spatial dimension, two neighboring trails may combine to form a larger one, and also one trail may split into two. In addition, trails move diffusively. The model is defined by two parameters which quantify the fragmentation rate and the fragment size. In the long-time limit, the system reaches a steady state, and our focus is the limiting distribution of trail weights. We find that the density of trail weight has power-law tail P(w)~w –γ formore » small weight w. We obtain the exponent γ analytically and find that it varies continuously with the two model parameters. In conclusion, the exponent γ can be positive or negative, so that in one range of parameters small-weight trails are abundant and in the complementary range they are rare.« less

  15. Rediscovering a Stream in Danville, Kentucky: The Clark Run Corridor and Trails Project.

    ERIC Educational Resources Information Center

    Bright, Debra

    1998-01-01

    In Danville, Kentucky, the community cooperated in converting a neglected stream into a walking trail and park network, resulting in increased community pride, environmental awareness, community revitalization, and public-private cooperation. The local middle school regularly tests the water, and the local college's annual clean-up party earned an…

  16. Recreation trails in Maine and New Hampshire: A comparison of notorized, non-motorized, and non-mechanized trails

    Treesearch

    Ethel Wilkerson; Andrew. Whitman

    2010-01-01

    We sampled 112 trail segments in Maine and New Hampshire to assess the impact of motorized and non-motorized recreation on trail conditions and stream sedimentation. On each segment, we assessed physical trail conditions (width, cross-sectional area, occurrence of excessively muddy and rutted/eroded sections), presence of trash, and sedimentation at stream crossings....

  17. Hiking shared-use single-track trails: a look at hikers and hunters along the Falls Lake Trail

    Treesearch

    Christopher M. Snow; Roger L. Moore

    2007-01-01

    The Falls Lake Trail, a 26.8-mile, single-track pedestrian trail located near the Research Triangle Region of North Carolina, traverses lands managed by the United States Army Corps of Engineers; North Carolina Division of Parks and Recreation; North Carolina Division of Wildlife Resources; and Wake County Parks, Recreation and Open Space. The non-profit trail advocacy...

  18. An analysis of state legislation on community trails.

    PubMed

    Eyler, Amy; Lankford, Tina; Chriqui, Jamie; Evenson, Kelly R; Kruger, Judy; Tompkins, Nancy; Voorhees, Carolyn; Zieff, Susan; Aytur, Semra; Brownson, Ross

    2010-03-01

    Trails provide opportunities for recreation, transportation and activity. The purpose of this article is to describe state legislation related to community trails, to analyze legislation content, and to evaluate legislation on inclusion of evidence-informed elements. State trail legislation from 2001 to 2008 was identified using online legislative databases. An analysis of evidence-informed elements included in the legislation was conducted. These elements included: funding, liability, accessibility, connectivity, and maintenance. Of the total 991 trail bills, 516 (52.0%) were appropriations bills, of which 167 (32.2%) were enacted. We analyzed 475 (48%) nonappropriation trail bills of which 139 (29.3%) were enacted. The percentage of enactment of appropriations bills decreased over time while enactment of nonappropriations trail bills increased. Over half of the nonappropriations trail bills included at least 1 evidence-informed element, most commonly funding. Few bills contained liability, connectivity, accessibility, or maintenance. There is opportunity for providing evidence-informed information to policy-makers to potentially influence bill content. The number of bills with a funding element demonstrates that fiscal support for trails is an important policy lever that state legislatures may use to support trails. Lastly, trails should be considered in over-all state-level physical activity legislation to provide opportunities for communities to be active.

  19. AUTOMATIC DIRT TRAIL ANALYSIS IN DERMOSCOPY IMAGES

    PubMed Central

    Cheng, Beibei; Stanley, R. Joe; Stoecker, William V.; Osterwise, Christopher T.P.; Stricklin, Sherea M.; Hinton, Kristen A.; Moss, Randy H.; Oliviero, Margaret; Rabinovitz, Harold S.

    2011-01-01

    Basal cell carcinoma (BCC) is the most common cancer in the U.S. Dermatoscopes are devices used by physicians to facilitate the early detection of these cancers based on the identification of skin lesion structures often specific to BCCs. One new lesion structure, referred to as dirt trails, has the appearance of dark gray, brown or black dots and clods of varying sizes distributed in elongated clusters with indistinct borders, often appearing as curvilinear trails. In this research, we explore a dirt trail detection and analysis algorithm for extracting, measuring, and characterizing dirt trails based on size, distribution, and color in dermoscopic skin lesion images. These dirt trails are then used to automatically discriminate BCC from benign skin lesions. For an experimental data set of 35 BCC images with dirt trails and 79 benign lesion images, a neural network-based classifier achieved a 0.902 area under a receiver operating characteristic curve using a leave-one-out approach, demonstrating the potential of dirt trails for BCC lesion discrimination. PMID:22233099

  20. 30 CFR 75.600 - Trailing cables; flame resistance.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Trailing cables; flame resistance. 75.600... SAFETY AND HEALTH MANDATORY SAFETY STANDARDS-UNDERGROUND COAL MINES Trailing Cables § 75.600 Trailing cables; flame resistance. [Statutory Provisions] Trailing cables used in coal mines shall meet the...

  1. 30 CFR 75.606 - Protection of trailing cables.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Protection of trailing cables. 75.606 Section... AND HEALTH MANDATORY SAFETY STANDARDS-UNDERGROUND COAL MINES Trailing Cables § 75.606 Protection of trailing cables. [Statutory Provisions] Trailing cables shall be adequately protected to prevent damage by...

  2. Hiker preferences for trail features and maps

    Treesearch

    Roger E. McCay

    1978-01-01

    Hikers at a Pennsylvania state park were asked what items were essential to their trail experience. From a list of 18 items, an overwhelming majority of hikers wanted to see trail names and directional signs along a natural surfaced trail.

  3. Wicket gate trailing-edge blowing: A method for improving off-design hydroturbine performance by adjusting the runner inlet swirl angle

    NASA Astrophysics Data System (ADS)

    Lewis, B. J.; Cimbala, J. M.; Wouden, A. M.

    2014-03-01

    At their best efficiency point (BEP), hydroturbines operate at very high efficiency. However, with the ever-increasing penetration of alternative electricity generation, it has become common to operate hydroturbines at off-design conditions in order to maintain stability in the electric power grid. This paper demonstrates a method for improving hydroturbine performance during off-design operation by injecting water through slots at the trailing edges of the wicket gates. The injected water causes a change in bulk flow direction at the inlet of the runner. This change in flow angle from the wicket gate trailing-edge jets provides the capability of independently varying the flow rate and swirl angle through the runner, which in current designs are both determined by the wicket gate opening angle. When properly tuned, altering the flow angle results in a significant improvement in turbine efficiency during off-design operation.

  4. 30 CFR 75.826 - High-voltage trailing cables.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false High-voltage trailing cables. 75.826 Section 75...-Voltage Longwalls § 75.826 High-voltage trailing cables. High-voltage trailing cables must: (a) Meet existing trailing cable requirements and the approval requirements of the high-voltage continuous mining...

  5. 30 CFR 77.604 - Protection of trailing cables.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Protection of trailing cables. 77.604 Section 77.604 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR COAL MINE SAFETY... MINES Trailing Cables § 77.604 Protection of trailing cables. Trailing cables shall be adequately...

  6. 36 CFR 212.21 - Pacific Crest National Scenic Trail.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Trail. 212.21 Section 212.21 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF... National Scenic Trail. The Pacific Crest National Scenic Trail as defined by the National Trails Systems Act, 82 Stat. 919, shall be administered primarily as a footpath and horseback riding trail by the...

  7. Active flow control for a blunt trailing edge profiled body

    NASA Astrophysics Data System (ADS)

    Naghib Lahouti, Arash

    Flow in the wake of nominally two-dimensional bluff bodies is dominated by vortex shedding, beyond a very small threshold Reynolds number. Vortex shedding poses challenges in the design of structures, due to its adverse effects such as cyclic aerodynamic loads and fatigue. The wake vortices are often accompanied by large- and small-scale secondary instabilities, which manifest as dislocations in the primary wake vortices, and/or pairs of counter-rotating streamwise vortices, depending on the dominant instability mode(s), which in turn depends on the profile geometry and Reynolds number. The secondary instabilities interact with the wake vortices through several mechanisms. Therefore, manipulation of the secondary instabilities can be used as a means to alter the wake vortices, in order to reduce their adverse effects. In the present study, flow in the wake of a blunt trailing edge profiled body, composed of an elliptical leading edge and a rectangular trailing edge, has been studied at Reynolds numbers ranging from Re(d) = 500 to 2150 where d is thickness of the body, to identify the secondary instabilities. Various tools, including numerical simulations, Laser Induced Fluorescence (LIF), and Particle Image Velocimetry (PIV) have been used for this study. Proper Orthogonal Decomposition (POD) has been applied to analyze the velocity field data. The results indicate the existence of small-scale instabilities with a spanwise wavelength of 2.0d to 2.5d in the near wake. The mechanism of the instability is similar to the Mode-A instability of a circular cylinder; however, it displays features that are specific to the blunt trailing edge profiled body. An active three-dimensional flow control mechanism based on the small-scale instabilities has been designed and evaluated. The mechanism comprises a series of trailing edge injection ports, with a spanwise spacing equal to the wavelength of the small-scale instabilities. Following preliminary evaluation of the control

  8. 30 CFR 77.606 - Energized trailing cables; handling.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Energized trailing cables; handling. 77.606... COAL MINES Trailing Cables § 77.606 Energized trailing cables; handling. Energized medium- and high-voltage trailing cables shall be handled only by persons wearing protective rubber gloves (see § 77.606-1...

  9. TRAIL-CM4 fusion protein shows in vitro antibacterial activity and a stronger antitumor activity than solo TRAIL protein.

    PubMed

    Sang, Ming; Zhang, Jiaxin; Li, Bin; Chen, Yuqing

    2016-06-01

    A TRAIL-CM4 fusion protein in soluble form with tumor selective apoptosis and antibacterial functions was expressed in the Escherichia coli expression system and isolated through dialysis refolding and histidine-tag Nickel-affinity purification. Fresh Jurkat cells were treated with the TRAIL-CM4 fusion protein. Trypan blue staining and MTT analyses showed that, similar to a TRAIL positive control, Jurkat cell proliferation was significantly inhibited. Flow cytometry analyses using Annexin V-fluorescein revealed that Jurkat cells treated with the TRAIL-CM4 fusion protein exhibited increased apoptosis. Laser confocal microscopy showed that APB-CM4 and the fusion protein TRAIL-CM4 can bind to Jurkat cell membranes and initiate their destruction. ABP-CM4 enhances the antitumor activity of TRAIL by targeting and damaging the tumor cell membrane. In antibacterial experiments, agar well diffusion and bacterial growth inhibition curve assays revealed concentration-dependent TRAIL-CM4 antibacterial activity against Escherichia coli K12D31. The expressed TRAIL-CM4 fusion protein exhibited enhanced antitumor and antibacterial activities. Fusion protein expression allowed the two different proteins to function in combination. Copyright © 2016 Elsevier Inc. All rights reserved.

  10. Use of chemical soil additives to stabilize off-road vehicle trails

    Treesearch

    J.N. Davis; J.E. Baier; J.P. Fulton; D.A. Brown; T.P. McDonald

    2007-01-01

    Off‐road vehicle (ORV) use is an increasingly popular form of outdoor recreation throughout the United States. This form of motorized recreation, however, can sometimes lead to serious erosion of trail running surfaces, with resulting export of sediment into forested ecosystems causing environmental degradation. This project was conducted to determine the...

  11. 36 CFR 261.55 - National Forest System trails.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false National Forest System trails... PROHIBITIONS Prohibitions in Areas Designated by Order § 261.55 National Forest System trails. When provided by... National Forest System trail: (a) Being on a trail. (b) Using any type of vehicle prohibited by the order...

  12. 30 CFR 57.4057 - Underground trailing cables.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... and Control § 57.4057 Underground trailing cables. Underground trailing cables shall be accepted or... 30 Mineral Resources 1 2013-07-01 2013-07-01 false Underground trailing cables. 57.4057 Section 57.4057 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR METAL AND NONMETAL...

  13. 30 CFR 57.4057 - Underground trailing cables.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... and Control § 57.4057 Underground trailing cables. Underground trailing cables shall be accepted or... 30 Mineral Resources 1 2012-07-01 2012-07-01 false Underground trailing cables. 57.4057 Section 57.4057 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR METAL AND NONMETAL...

  14. 30 CFR 57.4057 - Underground trailing cables.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... and Control § 57.4057 Underground trailing cables. Underground trailing cables shall be accepted or... 30 Mineral Resources 1 2014-07-01 2014-07-01 false Underground trailing cables. 57.4057 Section 57.4057 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR METAL AND NONMETAL...

  15. 30 CFR 57.4057 - Underground trailing cables.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... and Control § 57.4057 Underground trailing cables. Underground trailing cables shall be accepted or... 30 Mineral Resources 1 2011-07-01 2011-07-01 false Underground trailing cables. 57.4057 Section 57.4057 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR METAL AND NONMETAL...

  16. HUBBLE: ON THE ASTEROID TRAIL

    NASA Technical Reports Server (NTRS)

    2002-01-01

    Astronomers Karl Stapelfeldt and Robin Evans have tracked down about 100 small asteroids by hunting through more than 28,000 archival images taken by the Hubble Space Telescope's Wide Field and Planetary Camera 2. Here is a sample of what they have found: four archival images that show the curved trails left by asteroids. [Top left]: Hubble captured a bright asteroid, with a visual magnitude of 18.7, roaming in the constellation Centaurus. Background stars are shown in white, while the asteroid trail is depicted in blue at top center. The trail has a length of 19 arc seconds. This asteroid has a diameter of one and one-quarter miles (2 kilometers), and was located 87 million miles from Earth and 156 million miles from the sun. Numerous orange and blue specks in this image and the following two images were created by cosmic rays, energetic subatomic particles that struck the camera's detector. [Top right]: Here is an asteroid with a visual magnitude of 21.8 passing a galaxy in the constellation Leo. The trail is seen in two consecutive exposures, the first shown in blue and the second in red. This asteroid has a diameter of half a mile (0.8 kilometers), and was located 188 million miles from Earth and 233 million miles from the sun. [Lower left]: This asteroid in the constellation Taurus has a visual magnitude of 23, and is one of the faintest seen so far in the Hubble archive. It moves from upper right to lower left in two consecutive exposures; the first trail is shown in blue and the second in red. Because of the asteroid's relatively straight trail, astronomers could not accurately determine its distance. The estimated diameter is half a mile (0.8 kilometers) at an Earth distance of 205 million miles and a sun distance of 298 million miles. [Lower right]: This is a broken asteroid trail crossing the outer regions of galaxy NGC 4548 in Coma Berenices. Five trail segments (shown in white) were extracted from individual exposures and added to a cleaned color image

  17. Pheromone disruption of Argentine ant trail integrity.

    PubMed

    Suckling, D M; Peck, R W; Manning, L M; Stringer, L D; Cappadonna, J; El-Sayed, A M

    2008-12-01

    Disruption of Argentine ant trail following and reduced ability to forage (measured by bait location success) was achieved after presentation of an oversupply of trail pheromone, (Z)-9-hexadecenal. Experiments tested single pheromone point sources and dispersion of a formulation in small field plots. Ant walking behavior was recorded and digitized by using video tracking, before and after presentation of trail pheromone. Ants showed changes in three parameters within seconds of treatment: (1) Ants on trails normally showed a unimodal frequency distribution of walking track angles, but this pattern disappeared after presentation of the trail pheromone; (2) ants showed initial high trail integrity on a range of untreated substrates from painted walls to wooden or concrete floors, but this was significantly reduced following presentation of a point source of pheromone; (3) the number of ants in the pheromone-treated area increased over time, as recruitment apparently exceeded departures. To test trail disruption in small outdoor plots, the trail pheromone was formulated with carnuba wax-coated quartz laboratory sand (1 g quartz sand/0.2 g wax/1 mg pheromone). The pheromone formulation, with a half-life of 30 h, was applied by rotary spreader at four rates (0, 2.5, 7.5, and 25 mg pheromone/m(2)) to 1- and 4-m(2) plots in Volcanoes National Park, Hawaii. Ant counts at bait cards in treated plots were significantly reduced compared to controls on the day of treatment, and there was a significant reduction in ant foraging for 2 days. These results show that trail pheromone disruption of Argentine ants is possible, but a much more durable formulation is needed before nest-level impacts can be expected.

  18. Trail-following in termites: Evidence for a multicomponent system.

    PubMed

    Kaib, M; Bruinsma, O; Leuthold, R H

    1982-09-01

    Several African termite species from different subfamilies and different habitats are sensitive to trail-active extracts or to naturally laid trails from other species. Using single-extract bioassays, it is shown that the response threshold for trail-following is nearly identical for all tested species (except forHodotermes mossambicus). However, when termite workers have a choice between trails from their own species and from other species, conspecific trail-following is exclusively observed. This phenomenon can be counteracted by dilution (1∶10) of the conspecific trail-pheromone extract. Tests of the trail activity of various synthetic alcohols show that among these, the highest sensitivity of termite workers is to (Z)-3-dodecen-1-ol. Based on our experimental data, we postulate that, in addition to a generally active trail-pheromone constituent (an unsaturated primary C12 alcohol) or a pool of chemically closely related alcohols, other species-specific components are present in termite trails.

  19. Trail-following behavior ofReticulitermes hesperus Banks (Isoptera: Rhinotermitidae).

    PubMed

    Grace, J K; Wood, D L; Frankie, G W

    1988-02-01

    The behavior ofReticulitermes hesperus Banks pseudergates (workers) was assessed on artificial trails containing different concentrations of sternal gland extract. On nongiadient trails, more pseudergates were recruited to trails of greater pheromone concentration, they traveled a greater distance without pausing, and their rate of locomotion increased over that observed on trails of lesser concentration (positive orthokinesis). Of the individuals pausing before completing trails of high concentration, fewer left the trails or reversed direction (negative klinokinesis) than on trails of lower concentration. Termites walking down concentration gradients failed to complete these trails to the low-concentration termini. At a point representing an average decrease of slightly more than 10-fold in the original concentration of pheromone, individuals reversed their direction of travel and returned to the high-concentration terminus. Termites walking up pheromone gradients proceeded to the high-concentration termini without reversing direction.R. hesperus pseudergates are thus able to orient along a gradient of trail pheromone by longitudinal klinotaxis.

  20. NASA aircraft trailing vortex research

    NASA Technical Reports Server (NTRS)

    Mcgowan, W. A.

    1971-01-01

    A brief description is given of NASA's comprehensive program to study the aircraft trailing vortex problem. Wind tunnel experiments are used to develop the detailed processes of wing tip vortex formation and explore different means to either prevent trailing vortices from forming or induce early break-up. Flight tests provide information on trailing vortex system behavior behind large transport aircraft, both near the ground, as in the vicinity of the airport, and at cruise/holding pattern altitudes. Results from some flight tests are used to show how pilots might avoid the dangerous areas when flying in the vicinity of large transport aircraft. Other flight tests will be made to verify and evaluate trailing vortex elimination schemes developed in the model tests. Laser Doppler velocimeters being developed for use in the research program and to locate and measure vortex winds in the airport area are discussed. Field tests have shown that the laser Doppler velocimeter measurements compare well with those from cup anemometers.

  1. 21 CFR 1311.215 - Internal audit trail.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 9 2010-04-01 2010-04-01 false Internal audit trail. 1311.215 Section 1311.215... ORDERS AND PRESCRIPTIONS (Eff. 6-1-10) Electronic Prescriptions § 1311.215 Internal audit trail. (a) The... with audit trail functions. (6) For application service providers, attempted or successful annotation...

  2. Travel to, and use of, twenty-one Michigan trails.

    PubMed

    Price, Anna E; Reed, Julian A; Grost, Lisa; Harvey, Christina; Mantinan, Karah

    2013-03-01

    This study examined trail use among 857 trail users on 21 trails in Michigan from 2008 to 2011 using a valid and reliable intercept survey. Most of the 857 participants traveled to the trail from their home (92.6%), lived within 15 min of the trails (74.8%), and used active transport to travel to the trails 69.7%. The odds of active transport to the trails were greater among those who had not graduated high school (OR=3.49; 95% CI=1.02, 11.99) and high school graduates (OR=7.432; 95% CI=2.02, 27.30) compared to college graduates. Whites and adults also had greater odds of active transport than non-Whites (OR=3.160, 95% CI: 1.65, 6.05), and older adults (OR=1.75; 95% CI: 1.20, 2.54). The majority of respondents (89.7%) reported using trails for recreational purposes. A significantly greater proportion of females (73.3%) compared to males (64.7%) reported using the trail with others. The findings from this study might enable health and parks and recreation professionals to better promote physical activity on trails. Copyright © 2013 Elsevier Inc. All rights reserved.

  3. Automatic dirt trail analysis in dermoscopy images.

    PubMed

    Cheng, Beibei; Joe Stanley, R; Stoecker, William V; Osterwise, Christopher T P; Stricklin, Sherea M; Hinton, Kristen A; Moss, Randy H; Oliviero, Margaret; Rabinovitz, Harold S

    2013-02-01

    Basal cell carcinoma (BCC) is the most common cancer in the US. Dermatoscopes are devices used by physicians to facilitate the early detection of these cancers based on the identification of skin lesion structures often specific to BCCs. One new lesion structure, referred to as dirt trails, has the appearance of dark gray, brown or black dots and clods of varying sizes distributed in elongated clusters with indistinct borders, often appearing as curvilinear trails. In this research, we explore a dirt trail detection and analysis algorithm for extracting, measuring, and characterizing dirt trails based on size, distribution, and color in dermoscopic skin lesion images. These dirt trails are then used to automatically discriminate BCC from benign skin lesions. For an experimental data set of 35 BCC images with dirt trails and 79 benign lesion images, a neural network-based classifier achieved a 0.902 are under a receiver operating characteristic curve using a leave-one-out approach. Results obtained from this study show that automatic detection of dirt trails in dermoscopic images of BCC is feasible. This is important because of the large number of these skin cancers seen every year and the challenge of discovering these earlier with instrumentation. © 2011 John Wiley & Sons A/S.

  4. Trail pheromone disruption of red imported fire ant.

    PubMed

    Suckling, David M; Stringer, Lloyd D; Bunn, Barry; El-Sayed, Ashraf M; Vander Meer, Robert K

    2010-07-01

    The fire ant, Solenopsis invicta (Hymenoptera: Formicidae), is considered one of the most aggressive and invasive species in the world. Toxic bait systems are used widely for control, but they also affect non-target ant species and cannot be used in sensitive ecosystems such as organic farms and national parks. The fire ant uses recruitment pheromones to organize the retrieval of large food resources back to the colony, with Z,E-alpha-farnesene responsible for the orientation of workers along trails. We prepared Z,E-alpha-farnesene, (91% purity) from extracted E,E-alpha-farnesene and demonstrated disruption of worker trail orientation after presentation of an oversupply of this compound from filter paper point sources (30 microg). Trails were established between queen-right colony cells and food sources in plastic tubs. Trail-following behavior was recorded by overhead webcam, and ants were digitized before and after presentation of the treatment, using two software approaches. The linear regression statistic, r(2) was calculated. Ants initially showed high linear trail integrity (r(2) = 0.75). Within seconds of presentation of the Z,E-alpha-farnesene treatment, the trailing ants showed little or no further evidence of trail following behavior in the vicinity of the pheromone source. These results show that trailing fire ants become disorientated in the presence of large amounts of Z,E-alpha-farnesene. Disrupting fire ant recruitment to resources may have a negative effect on colony size or other effects yet to be determined. This phenomenon was demonstrated recently for the Argentine ant, where trails were disrupted for two weeks by using their formulated trail pheromone, Z-9-hexadecenal. Further research is needed to establish the long term effects and control potential for trail disruption in S. invicta.

  5. Pheromone disruption of Argentine ant trail integrity

    USGS Publications Warehouse

    Suckling, D.M.; Peck, R.W.; Manning, L.M.; Stringer, L.D.; Cappadonna, J.; El-Sayed, A. M.

    2008-01-01

    Disruption of Argentine ant trail following and reduced ability to forage (measured by bait location success) was achieved after presentation of an oversupply of trail pheromone, (Z)-9-hexadecenal. Experiments tested single pheromone point sources and dispersion of a formulation in small field plots. Ant walking behavior was recorded and digitized by using video tracking, before and after presentation of trail pheromone. Ants showed changes in three parameters within seconds of treatment: (1) Ants on trails normally showed a unimodal frequency distribution of walking track angles, but this pattern disappeared after presentation of the trail pheromone; (2) ants showed initial high trail integrity on a range of untreated substrates from painted walls to wooden or concrete floors, but this was significantly reduced following presentation of a point source of pheromone; (3) the number of ants in the pheromone-treated area increased over time, as recruitment apparently exceeded departures. To test trail disruption in small outdoor plots, the trail pheromone was formulated with carnuba wax-coated quartz laboratory sand (1 g quartz sand/0.2 g wax/1 mg pheromone). The pheromone formulation, with a half-life of 30 h, was applied by rotary spreader at four rates (0, 2.5, 7.5, and 25 mg pheromone/m2) to 1- and 4-m2 plots in Volcanoes National Park, Hawaii. Ant counts at bait cards in treated plots were significantly reduced compared to controls on the day of treatment, and there was a significant reduction in ant foraging for 2 days. These results show that trail pheromone disruption of Argentine ants is possible, but a much more durable formulation is needed before nest-level impacts can be expected. ?? 2008 Springer Science+Business Media, LLC.

  6. 30 CFR 57.12088 - Splicing trailing cables.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Splicing trailing cables. 57.12088 Section 57... Underground Only § 57.12088 Splicing trailing cables. No splice, except a vulcanized splice or its equivalent, shall be made in a trailing cable within 25 feet of the machine unless the machine is equipped with a...

  7. Radio polarisation measurements of meteor trail echoes with BRAMS

    NASA Astrophysics Data System (ADS)

    Lamy, H.; Ranvier, S.; Anciaux, M.; Calders, S.; De Keyser, J.; Gamby, E.

    2012-04-01

    BRAMS, the Belgian RAdio Meteor Stations, is a network of radio receiving stations using forward scatter techniques to detect and characterize meteors. The transmitter is a dedicated beacon located in Dourbes in the south-west of Belgium. It emits towards the zenith a purely sinusoidal wave circularly polarised, at a frequency of 49.97 MHz and with a power of 150 watts. The main goals of the project are to compute meteoroid flux rates and trajectories. Most receiving stations are using a 3 element Yagi antenna and are therefore only sensitive to one polarisation. The station located in Uccle has also a crossed 3 element Yagi antenna and therefore allows measurements of horizontal and vertical polarisations. We present the preliminary radio polarisation measurements of meteor trail echoes and compare them with the theoretical predictions of Jones & Jones (1991) for oblique scattering of radio waves from meteor trails.

  8. Study on Trailing Edge Ramp of Supercritical Airfoil

    DTIC Science & Technology

    2016-03-30

    7 th Asia-Pacific International Symposium on Aerospace Technology, 25 – 27 November 2015, Cairns Study on Trailing Edge Ramp of Supercritical...China Abstract Trailing edge flow control method could improve the performance of supercritical airfoil with a small modification on the original...airfoil. In this paper, a ramp of 2%~7% chord length is sliced near the trailing edge to improve airfoil performance. The trailing edge ramp is

  9. TRIPPy: Python-based Trailed Source Photometry

    NASA Astrophysics Data System (ADS)

    Fraser, Wesley C.; Alexandersen, Mike; Schwamb, Megan E.; Marsset, Michael E.; Pike, Rosemary E.; Kavelaars, JJ; Bannister, Michele T.; Benecchi, Susan; Delsanti, Audrey

    2016-05-01

    TRIPPy (TRailed Image Photometry in Python) uses a pill-shaped aperture, a rectangle described by three parameters (trail length, angle, and radius) to improve photometry of moving sources over that done with circular apertures. It can generate accurate model and trailed point-spread functions from stationary background sources in sidereally tracked images. Appropriate aperture correction provides accurate, unbiased flux measurement. TRIPPy requires numpy, scipy, matplotlib, Astropy (ascl:1304.002), and stsci.numdisplay; emcee (ascl:1303.002) and SExtractor (ascl:1010.064) are optional.

  10. Decoy receptors block TRAIL sensitivity at a supracellular level: the role of stromal cells in controlling tumour TRAIL sensitivity.

    PubMed

    O'Leary, L; van der Sloot, A M; Reis, C R; Deegan, S; Ryan, A E; Dhami, S P S; Murillo, L S; Cool, R H; Correa de Sampaio, P; Thompson, K; Murphy, G; Quax, W J; Serrano, L; Samali, A; Szegezdi, E

    2016-03-10

    Tumour necrosis factor-related apoptosis-inducing ligand (TRAIL) is a death ligand cytokine known for its cytotoxic activity against malignantly transformed cells. TRAIL induces cell death through binding to death receptors DR4 and DR5. The inhibitory decoy receptors (DcR1 and DcR2) co-expressed with death receptor 4 (DR4)/DR5 on the same cell can block the transmission of the apoptotic signal. Here, we show that DcRs also regulate TRAIL sensitivity at a supracellular level and thus represent a mechanism by which the microenvironment can diminish tumour TRAIL sensitivity. Mathematical modelling and layered or spheroid stroma-extracellular matrix-tumour cultures were used to model the tumour microenvironment. By engineering TRAIL to escape binding by DcRs, we found that DcRs do not only act in a cell-autonomous or cis-regulatory manner, but also exert trans-cellular regulation originating from stromal cells and affect tumour cells, highlighting the potent inhibitory effect of DcRs in the tumour tissue and the necessity of selective targeting of the two death-inducing TRAIL receptors to maximise efficacy.

  11. 30 CFR 75.604 - Permanent splicing of trailing cables.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Permanent splicing of trailing cables. 75.604... SAFETY AND HEALTH MANDATORY SAFETY STANDARDS-UNDERGROUND COAL MINES Trailing Cables § 75.604 Permanent splicing of trailing cables. [Statutory Provisions] When permanent splices in trailing cables are made...

  12. Hydrodynamic Trails Produced by Daphnia: Size and Energetics

    PubMed Central

    Wickramarathna, Lalith N.; Noss, Christian; Lorke, Andreas

    2014-01-01

    This study focuses on quantifying hydrodynamic trails produced by freely swimming zooplankton. We combined volumetric tracking of swimming trajectories with planar observations of the flow field induced by Daphnia of different size and swimming in different patterns. Spatial extension of the planar flow field along the trajectories was used to interrogate the dimensions (length and volume) and energetics (dissipation rate of kinetic energy and total dissipated power) of the trails. Our findings demonstrate that neither swimming pattern nor size of the organisms affect the trail width or the dissipation rate. However, we found that the trail volume increases with increasing organism size and swimming velocity, more precisely the trail volume is proportional to the third power of Reynolds number. This increase furthermore results in significantly enhanced total dissipated power at higher Reynolds number. The biggest trail volume observed corresponds to about 500 times the body volume of the largest daphnids. Trail-averaged viscous dissipation rate of the swimming daphnids vary in the range of to and the observed magnitudes of total dissipated power between and , respectively. Among other zooplankton species, daphnids display the highest total dissipated power in their trails. These findings are discussed in the context of fluid mixing and transport by organisms swimming at intermediate Reynolds numbers. PMID:24671019

  13. A mixed-modes approach for estimating hiking on trails through diverse forest landscapes: the case of the Appalachian Trail

    Treesearch

    Stanley J. Zarnoch; J.M. Bowker; H. Ken Cordell

    2011-01-01

    Many hiking trails traverse the forests and public lands across North America. It has therefore become important for federal management to gain an understanding of total use on these trails. However, there has never been a formal attempt to estimate hiking on these long, backcountry trails. This paper presents an approach that utilizes two survey instruments (exit-site...

  14. Chemical trail marking and following by caterpillars ofMalacosoma neustria.

    PubMed

    Peterson, S C

    1988-03-01

    Chemical trail marking and following by gregarious caterpillars,Malacosoma neustria L. (Lepidoptera: Lasiocampidae), was studied in the laboratory. As in other species ofMalacosoma, larvae deposit a trail pheromone from a sternal secretory site when searching the host for food. Larvae in the vanguard of foraging columns establish chemical trails as they explore new territory. Marking behavior diminishes as successive unfed foragers utilize the trail. These exploratory trails are subsequently overmarked by fed larvae returning to the tent. Other foragers follow the trails of fed larvae in preference to trails of unfed larvae. Thus, like the eastern tent caterpillar,M. americanum, successful foragers ofM. neustria recruit colony-mates to feeding sites. The chemical activity of both recruitment and exploratory trails degrades slowly, suggesting that the trail pheromone ofM. neustria is a nonvolatile substance. Caterpillars ofM. neustria readily follow the nonvolatile trail pheromone which has been identified fromM. americanum, 5β-cholestane-3,24-dione.

  15. 30 CFR 75.603 - Temporary splice of trailing cable.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Temporary splice of trailing cable. 75.603... SAFETY AND HEALTH MANDATORY SAFETY STANDARDS-UNDERGROUND COAL MINES Trailing Cables § 75.603 Temporary splice of trailing cable. [Statutory Provision] One temporary splice may be made in any trailing cable...

  16. 30 CFR 77.602 - Permanent splicing of trailing cables.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Permanent splicing of trailing cables. 77.602... COAL MINES Trailing Cables § 77.602 Permanent splicing of trailing cables. When permanent splices in trailing cables are made, they shall be: (a) Mechanically strong with adequate electrical conductivity; (b...

  17. Cold PSM, but not TRAIL, triggers autophagic cell death: A therapeutic advantage of PSM over TRAIL.

    PubMed

    Ito, Tomohisa; Ando, Takashi; Suzuki-Karasaki, Miki; Tokunaga, Tomohiko; Yoshida, Yukihiro; Ochiai, Toyoko; Tokuhashi, Yasuaki; Suzuki-Karasaki, Yoshihiro

    2018-05-21

    Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and cold plasma-stimulated medium (PSM) are promising novel anticancer tools due to their strong anticancer activities and high tumor-selectivity. The present study demonstrated that PSM and TRAIL may trigger autophagy in human malignant melanoma and osteosarcoma cells. Live-cell imaging revealed that even under nutritional and stress-free conditions, these cells possessed a substantial level of autophagosomes, which were localized in the cytoplasm separately from tubular mitochondria. In response to cytotoxic levels of PSM, the mitochondria became highly fragmented, and aggregated and colocalized with the autophagosomes. The cytotoxic effects of PSM were suppressed in response to various pharmacological autophagy inhibitors, including 3-methyladenine (3-MA) and bafilomycin A1, thus indicating the induction of autophagic cell death (ACD). Lethal levels of PSM also resulted in non-apoptotic, non-autophagic cell death in a reactive oxygen species-dependent manner under certain circumstances. Furthermore, TRAIL exhibited only a modest cytotoxicity toward these tumor cells, and did not induce ACD and mitochondrial aberration. The combined use of TRAIL and subtoxic concentrations of 3-MA resulted in decreased basal autophagy, increased mitochondrial aberration, colocalization with autophagosomes and apoptosis. These results indicated that PSM may induce ACD, whereas TRAIL may trigger cytoprotective autophagy that compromises apoptosis. To the best of our knowledge, the present study is the first to demonstrate that PSM can induce ACD in human cancer cells. These findings provide a rationale for the advantage of PSM over TRAIL in the destruction of apoptosis-resistant melanoma and osteosarcoma cells.

  18. Identification of TRAIL-inducing compounds highlights small molecule ONC201/TIC10 as a unique anti-cancer agent that activates the TRAIL pathway.

    PubMed

    Allen, Joshua E; Krigsfeld, Gabriel; Patel, Luv; Mayes, Patrick A; Dicker, David T; Wu, Gen Sheng; El-Deiry, Wafik S

    2015-05-01

    We previously reported the identification of ONC201/TIC10, a novel small molecule inducer of the human TRAIL gene that improves efficacy-limiting properties of recombinant TRAIL and is in clinical trials in advanced cancers based on its promising safety and antitumor efficacy in several preclinical models. We performed a high throughput luciferase reporter screen using the NCI Diversity Set II to identify TRAIL-inducing compounds. Small molecule-mediated induction of TRAIL reporter activity was relatively modest and the majority of the hit compounds induced low levels of TRAIL upregulation. Among the candidate TRAIL-inducing compounds, TIC9 and ONC201/TIC10 induced sustained TRAIL upregulation and apoptosis in tumor cells in vitro and in vivo. However, ONC201/TIC10 potentiated tumor cell death while sparing normal cells, unlike TIC9, and lacked genotoxicity in normal fibroblasts. Investigating the effects of TRAIL-inducing compounds on cell signaling pathways revealed that TIC9 and ONC201/TIC10, which are the most potent inducers of cell death, exclusively activate Foxo3a through inactivation of Akt/ERK to upregulate TRAIL and its pro-apoptotic death receptor DR5. These studies reveal the selective activity of ONC201/TIC10 that led to its selection as a lead compound for this novel class of antitumor agents and suggest that ONC201/TIC10 is a unique inducer of the TRAIL pathway through its concomitant regulation of the TRAIL ligand and its death receptor DR5.

  19. Nature Trails, Braille Trails, Foot Paths, Fragrance Gardens, Touch Museums for the Blind; Policy Statement.

    ERIC Educational Resources Information Center

    American Foundation for the Blind, New York, NY.

    The policy statement by the American Foundation for the Blind deals with nature trails, braille trails, foot paths, fragrance gardens, and touch museums for the blind. It is stated that the foundation approves of services such as provision of tape recorded guides and planting of fragrant shrubs which would benefit all users while recognizing…

  20. Looking for dark matter trails in colliding galaxy clusters

    NASA Astrophysics Data System (ADS)

    Harvey, David; Robertson, Andrew; Massey, Richard; Kneib, Jean-Paul

    2017-02-01

    If dark matter interacts, even weakly, via non-gravitational forces, simulations predict that it will be preferentially scattered towards the trailing edge of the halo during collisions between galaxy clusters. This will temporarily create a non-symmetric mass profile, with a trailing overdensity along the direction of motion. To test this hypothesis, we fit (and subtract) symmetric haloes to the weak gravitational data of 72 merging galaxy clusters observed with the Hubble Space Telescope. We convert the shear directly into excess κ and project in to a one-dimensional profile. We generate numerical simulations and find that the one-dimensional profile is well described with simple Gaussian approximations. We detect the weak lensing signal of trailing gas at a 4σ confidence, finding a mean gas fraction of Mgas/Mdm = 0.13 ± 0.035. We find no evidence for scattered dark matter particles with an estimated scattering fraction of f = 0.03 ± 0.05. Finally, we find that if we can reduce the statistical error on the positional estimate of a single dark matter halo to <2.5 arcsec, then we will be able to detect a scattering fraction of 10 per cent at the 3σ level with current surveys. This potentially interesting new method can provide an important independent test for other complimentary studies of the self-interaction cross-section of dark matter.

  1. 30 CFR 57.12003 - Trailing cable overload protection.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Trailing cable overload protection. 57.12003... Electricity Surface and Underground § 57.12003 Trailing cable overload protection. Individual overload protection or short circuit protection shall be provided for the trailing cables of mobile equipment. ...

  2. Assessing soil erosion on trails: A comparison of techniques

    Treesearch

    Mark C. Jewell; William E. Hammitt

    2000-01-01

    Reports of trail degradation have been increasing in different wildernesses. This impact has become a common concern among managers. Deteriorating tread conditions of trails are increasing, as is concern at protected areas worldwide. In order to make objective and timely trail resource decisions, managers need to have effective and efficient methods of assessing trail...

  3. Airbag Trails

    NASA Technical Reports Server (NTRS)

    2004-01-01

    This segment of the first color image from the panoramic camera on the Mars Exploration Rover Spirit shows the rover's airbag trails. These depressions in the soil were made when the airbags were deflated and retracted after landing.

  4. 30 CFR 57.12038 - Attachment of trailing cables.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Attachment of trailing cables. 57.12038 Section 57.12038 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR METAL AND... Electricity Surface and Underground § 57.12038 Attachment of trailing cables. Trailing cables shall be...

  5. Trails at LANL - Public Meeting and Forum - July 26, 2016

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Pava, Daniel Seth

    2016-07-26

    These are the slides of a meeting about trails at Los Alamos National Laboratory. The meeting goals are the folllowing: to inform and educate citizens about LANL trails management issues that include resource protection, safety, security and trails etiquette; to explain how and why LANL trails can be closed and reopened; and to understand your concerns and ideas about LANL trails use.

  6. Mechanism of trail following by the arboreal termite Nasutitermes corniger (Isoptera: Termitidae).

    PubMed

    Gazai, Vinícius; Bailez, Omar; Viana-Bailez, Ana Maria

    2014-01-01

    In this study, we investigated the mechanisms used by the arboreal termite Nasutitermes corniger (Motschulsky, 1855) to follow trails from the nest to sources of food. A plate containing one of seven trail types was used to connect an artificial nest of N. corniger with an artificial foraging arena. The trail types were: termite trail; paraffined termite trail; trail made of paraffin; rectal fluid extract trail; sternal gland extract trail; feces extract trail; and solvent trail (control). In each test, the time was recorded from the start of the test until the occurrence of trail following, at which point the number of termites that followed the trail for least 5 cm in the first 3 min of observation was recorded. The delay for termites initiating trail following along the termite trail was lower (0.55 ± 0.16 min) than in the trails of sternal gland extract (1.05 ± 0.08 min) and trails of termite feces extract (1.57 ± 0.21 min) (F(2), (48) = 22.59, P < 0.001). The number of termites that followed the termite trail was greater (207.3 ± 17.3) than the number that followed the trail of termite feces extract (102.5 ± 9.4) or sternal gland extract (36, 9 ± 1.6) (F(2), (48) = 174.34, P < 0.001). Therefore, feces on the trail may play an important role alongside sternal gland pheromones in increasing the persistence of the trail.

  7. Biliary tract instillation of a SMAC mimetic induces TRAIL-dependent acute sclerosing cholangitis-like injury in mice

    PubMed Central

    Guicciardi, Maria Eugenia; Krishnan, Anuradha; Bronk, Steven F; Hirsova, Petra; Griffith, Thomas S; Gores, Gregory J

    2017-01-01

    Primary sclerosing cholangitis (PSC) is a cholestatic liver disease of unknown etiopathogenesis characterized by fibrous cholangiopathy of large and small bile ducts. Systemic administration of a murine TNF-related apoptosis-inducing ligand (TRAIL) receptor agonist induces a sclerosing cholangitis injury in C57BL/6 mice, suggesting endogenous TRAIL may contribute to sclerosing cholangitis syndromes. Cellular inhibitor of apoptosis proteins (cIAP-1 and cIAP-2) are negative regulators of inflammation and TRAIL receptor signaling. We hypothesized that if endogenous TRAIL promotes sclerosing cholangitis, then cIAP depletion should also induce this biliary tract injury. Herein, we show that cIAP protein levels are reduced in the interlobular bile ducts of human PSC livers. Downregulation of cIAPs in normal human cholangiocytes in vitro by use of a SMAC mimetic (SM) induces moderate, ripoptosome-mediated apoptosis and RIP1-independent upregulation of proinflammatory cytokines and chemokines. Cytokine and chemokine expression was mediated by the non-canonical activation of NF-κB. To investigate whether downregulation of cIAPs is linked to generation of a PSC-like phenotype, an SM was directly instilled into the mouse biliary tree. Twelve hours after biliary instillation, TUNEL-positive cholangiocytes were identified; 5 days later, PSC-like changes were observed in the SM-treated mice, including a fibrous cholangiopathy of the interlobular bile ducts, portal inflammation, significant elevation of serum markers of cholestasis and cholangiographic evidence of intrahepatic biliary tract injury. In contrast, TRAIL and TRAIL-receptor deficient mice showed no sign of cholangiopathy following SM intrabiliary injection. We conclude that in vivo antagonism of cIAPs in mouse biliary epithelial cells is sufficient to trigger cholangiocytes apoptosis and a proinflammatory response resulting in a fibrous cholangiopathy resembling human sclerosing cholangitis. Therefore, downregulation

  8. 36 CFR 7.100 - Appalachian National Scenic Trail.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Trail. 7.100 Section 7.100 Parks, Forests, and Public Property NATIONAL PARK SERVICE, DEPARTMENT OF THE INTERIOR SPECIAL REGULATIONS, AREAS OF THE NATIONAL PARK SYSTEM § 7.100 Appalachian National Scenic Trail...? (1) You may cross the Appalachian National Scenic Trail corridor by using established, State-approved...

  9. The Effect of Nozzle Trailing Edge Thickness on Jet Noise

    NASA Technical Reports Server (NTRS)

    Henderson, Brenda; Kinzie, Kevin; Haskin, Henry

    2004-01-01

    The effect of nozzle trailing edge thickness on broadband acoustic radiation and the production of tones is investigated for coannular nozzles. Experiments were performed for a core nozzle trailing edge thickness between 0.38 mm and 3.17 mm. The on-set of discrete tones was found to be predominantly affected by the velocity ratio, the ratio of the fan velocity to the core velocity, although some dependency on trailing edge thickness was also noted. For a core nozzle trailing edge thickness greater than or equal to 0.89 mm, tones were produced for velocity ratios between 0.91 and 1.61. For a constant nozzle trailing edge thickness, the frequency varied almost linearly with the core velocity. The Strouhal number based on the core velocity changed with nozzle trailing edge thickness and varied between 0.16 and 0.2 for the core nozzles used in the experiments. Increases in broadband noise with increasing trailing edge thickness were observed for tone producing and non-tone producing conditions. A variable thickness trailing edge (crenellated) nozzle resulted in no tonal production and a reduction of the broadband trailing edge noise relative to that of the corresponding constant thickness trailing edge.

  10. 17 CFR 37.205 - Audit trail.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... trading; and (iv) Identification of each account to which fills are allocated. (3) Electronic analysis capability. A swap execution facility's audit trail program shall include electronic analysis capability with respect to all audit trail data in the transaction history database. Such electronic analysis capability...

  11. Hydrodynamic trails produced by Daphnia: size and energetics.

    PubMed

    Wickramarathna, Lalith N; Noss, Christian; Lorke, Andreas

    2014-01-01

    This study focuses on quantifying hydrodynamic trails produced by freely swimming zooplankton. We combined volumetric tracking of swimming trajectories with planar observations of the flow field induced by Daphnia of different size and swimming in different patterns. Spatial extension of the planar flow field along the trajectories was used to interrogate the dimensions (length and volume) and energetics (dissipation rate of kinetic energy and total dissipated power) of the trails. Our findings demonstrate that neither swimming pattern nor size of the organisms affect the trail width or the dissipation rate. However, we found that the trail volume increases with increasing organism size and swimming velocity, more precisely the trail volume is proportional to the third power of Reynolds number. This increase furthermore results in significantly enhanced total dissipated power at higher Reynolds number. The biggest trail volume observed corresponds to about 500 times the body volume of the largest daphnids. Trail-averaged viscous dissipation rate of the swimming daphnids vary in the range of 1.8 x 10(-6) W/kg to 3.4 x 10(-6) W/kg and the observed magnitudes of total dissipated power between 1.3 x 10(-9) W and 1 x 10(-8) W, respectively. Among other zooplankton species, daphnids display the highest total dissipated power in their trails. These findings are discussed in the context of fluid mixing and transport by organisms swimming at intermediate Reynolds numbers.

  12. Aeroelastic analysis of an adaptive trailing edge with a smart elastic skin

    NASA Astrophysics Data System (ADS)

    Arena, Maurizio; Pecora, Rosario; Amoroso, Francesco; Noviello, Maria Chiara; Rea, Francesco; Concilio, Antonio

    2017-09-01

    Nowadays, the design choices of the new generation aircraft are moving towards the research and development of innovative technologies, aimed at improving performance as well as to minimize the environmental impact. In the current "greening" context, the morphing structures represent a very attractive answer to such requirements: both aerodynamic and structural advantages are ensured in several flight conditions, safeguarding the fuel consumption at the same time. An aeronautical intelligent system is therefore the outcome of combining complex smart materials and structures, assuring the best functionality level in the flight envelope. The Adaptive Trailing Edge Device (ATED) is a sub-project inside SARISTU (Smart Intelligent Aircraft Structures), an L2 level project of the 7th EU Framework programme coordinated by Airbus, aimed at developing technologies for realizing a morphing wing extremity addressed to improve the general aircraft performance and to reduce the fuel burning up to 5%. This specific study, divided into design, manufacturing and testing phases, involved universities, research centers and leading industries of the European consortium. The paper deals with the aeroelastic impact assessment of a full-scale morphing wing trailing edge on a Large Aeroplanes category aircraft. The FE (Finite Element) model of the technology demonstrator, located in the aileron region and manufactured within the project, was referenced to for the extrapolation of the structural properties of the whole adaptive trailing edge device placed in its actual location in the outer wing. The input FE models were processed within MSC-Nastran® environment to estimate stiffness and inertial distributions suitable to construct the aeroelastic stick-beam mock-up of the reference structure. Afterwards, a flutter analysis in simulated operative condition, have been carried out by means of Sandy®, an in-house code, according to meet the safety requirements imposed by the applicable

  13. TRIPPy: Trailed Image Photometry in Python

    NASA Astrophysics Data System (ADS)

    Fraser, Wesley; Alexandersen, Mike; Schwamb, Megan E.; Marsset, Michaël; Pike, Rosemary E.; Kavelaars, J. J.; Bannister, Michele T.; Benecchi, Susan; Delsanti, Audrey

    2016-06-01

    Photometry of moving sources typically suffers from a reduced signal-to-noise ratio (S/N) or flux measurements biased to incorrect low values through the use of circular apertures. To address this issue, we present the software package, TRIPPy: TRailed Image Photometry in Python. TRIPPy introduces the pill aperture, which is the natural extension of the circular aperture appropriate for linearly trailed sources. The pill shape is a rectangle with two semicircular end-caps and is described by three parameters, the trail length and angle, and the radius. The TRIPPy software package also includes a new technique to generate accurate model point-spread functions (PSFs) and trailed PSFs (TSFs) from stationary background sources in sidereally tracked images. The TSF is merely the convolution of the model PSF, which consists of a moffat profile, and super-sampled lookup table. From the TSF, accurate pill aperture corrections can be estimated as a function of pill radius with an accuracy of 10 mmag for highly trailed sources. Analogous to the use of small circular apertures and associated aperture corrections, small radius pill apertures can be used to preserve S/Ns of low flux sources, with appropriate aperture correction applied to provide an accurate, unbiased flux measurement at all S/Ns.

  14. Trail impact monitoring in Rocky Mountain National Park, USA

    NASA Astrophysics Data System (ADS)

    Svajda, J.; Korony, S.; Brighton, I.; Esser, S.; Ciapala, S.

    2016-01-01

    This paper examines impacts of increased visitation leading to human trampling of vegetation and soil along several trails in Rocky Mountain National Park (RMNP) to understand how abiotic factors and level of use can influence trail conditions. RMNP is one of the most visited national parks in the USA, with 3.3 million visitors in 2012 across 1075 km2 and 571 km of hiking trails. 95 % of the park is designated wilderness, making the balance between preservation and visitor use challenging. This research involves the application of trail condition assessments to 56 km of trails to determine prevailing factors and what, if any, connection between them exist. The study looked at a variety of inventory and impact indicators and standards to determine their importance and to develop a baseline condition of trails. The data can be used for future comparison and evaluation of development trends. We found that trail widening (mean trail width 88.9 cm) and soil loss (cross-sectional area 172.7 cm2) are the most visible effects of trail degradation. Further statistical analyses of data identified the role and influence of various factors (e.g., use level and topography). Insights into the influence of these factors can lead to the selection of appropriate management measures to avoid or minimize negative consequences from increased visitation.

  15. Trail impact monitoring in Rocky Mountain National Park, USA

    NASA Astrophysics Data System (ADS)

    Svajda, J.; Korony, S.; Brighton, I.; Esser, S.; Ciapala, S.

    2015-11-01

    This paper examines impacts of increased visitation leading to human trampling of vegetation and soil along several trails in Rocky Mountain National Park (RMNP) to understand how abiotic factors and level of use can influence trail conditions. RMNP is one of the most visited national parks in the USA with 3.3 million visitors in 2012 across 1075 km2 and 571 km of hiking trails. 95 % of the park is designated wilderness making the balance between preservation and visitor use challenging. This research involves the application of trail condition assessments to 56 km of trails to determine prevailing factors and what, if any, connection between them exist. The study looked at a variety of inventory and impact indicators and standards to determine their importance and to develop a baseline condition of trails. The data can be used for future comparison and evaluation of development trends. We found that trail widening (mean trail width 88.9 cm) and soil loss (cross sectional area 172.7 cm2) are the most visible effects of trail degradation. Further statistical analyses of data identified the role and influence of various factors (e.g. use level and topography). Insights into the influence of these factors can lead to the selection of appropriate management measures to avoid or minimize negative consequences from increased visitation.

  16. Use and Nonuse of a Rail Trail Conversion for Physical Activity: Implications for Promoting Trail Use

    ERIC Educational Resources Information Center

    Price, Anna E.; Reed, Julian A.

    2014-01-01

    Background: There is limited research examining both use and nonuse of trails for physical activity. Purpose: Such research might enable health educators to better promote physical activity on trails.Methods:We used random digit dialing methods to survey 726 respondents in 2012. Results: The majority (75.1%) of respondents reported not using the…

  17. 49 CFR 236.776 - Movement, trailing.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false Movement, trailing. 236.776 Section 236.776 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL RAILROAD ADMINISTRATION... Movement, trailing. The movement of a train over the points of a switch which face in the direction in...

  18. Final Scientific/Technical Report for project “Geomechanical Monitoring for CO 2 Hub Storage: Production and Injection at Kevin Dome

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Daley, Thomas M.; Vasco, Don; Ajo-Franklin, Jonathan

    After learning that the TDS value in the target injection formation at the Kevin Dome site is too low to qualify for an EPA Class VI CO2 injection permit, the BSCSP project was re-scoped such that injection of CO2 is no longer planned. With no injection planned, the Geomechanics project was closed. In this final report, we describe the objective and approach of the project as proposed, and the limited results obtained before stopping work. The objective of the proposed research was the development & validation of an integrated monitoring approach for quantifying the interactions between large-scale geological carbon storagemore » (GCS) and subsurface geomechanical state, particularly perturbations relevant to reservoir integrity such as fault reactivation and induced fracturing. In the short period of work before knowing the fate of the Kevin Dome project, we (1) researched designs for both the proposed InSAR corner reflectors as well as the near-surface 3C seismic stations; (2) developed preliminary elastic geomechanical models; (3) developed a second generation deformation prediction for the BSCSP Kevin Dome injection site; and (4) completed a preliminary map of InSAR monuments and shallow MEQ wells in the vicinity of the BSCSP injection pad.« less

  19. 33 CFR 117.401 - Trail Creek.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Trail Creek. 117.401 Section 117.401 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY BRIDGES DRAWBRIDGE OPERATION REGULATIONS Specific Requirements Indiana § 117.401 Trail Creek. (a) The draw of the Franklin...

  20. 30 CFR 75.601 - Short circuit protection of trailing cables.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Short circuit protection of trailing cables. 75... MINE SAFETY AND HEALTH MANDATORY SAFETY STANDARDS-UNDERGROUND COAL MINES Trailing Cables § 75.601 Short circuit protection of trailing cables. [Statutory Provisions] Short circuit protection for trailing cables...

  1. 30 CFR 75.605 - Clamping of trailing cables to equipment.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Clamping of trailing cables to equipment. 75... MINE SAFETY AND HEALTH MANDATORY SAFETY STANDARDS-UNDERGROUND COAL MINES Trailing Cables § 75.605 Clamping of trailing cables to equipment. [Statutory Provisions] Trailing cables shall be clamped to...

  2. Multiuse trail intersection safety analysis: A crowdsourced data perspective.

    PubMed

    Jestico, Ben; Nelson, Trisalyn A; Potter, Jason; Winters, Meghan

    2017-06-01

    Real and perceived concerns about cycling safety are a barrier to increased ridership in many cities. Many people prefer to bike on facilities separated from motor vehicles, such as multiuse trails. However, due to underreporting, cities lack data on bike collisions, especially along greenways and multiuse paths. We used a crowdsourced cycling incident dataset (2005-2016) from BikeMaps.org for the Capital Regional District (CRD), BC, Canada. Our goal was to identify design characteristics associated with unsafe intersections between multiuse trails and roads. 92.8% of mapped incidents occurred between 2014 and 2016. We extracted both collision and near miss incidents at intersections from BikeMaps.org. We conducted site observations at 32 intersections where a major multiuse trail intersected with roads. We compared attributes of reported incidents at multiuse trail-road intersections to those at road-road intersections. We then used negative binomial regression to model the relationship between the number of incidents and the infrastructure characteristics at multiuse trail-road intersections. We found a higher proportion of collisions (38%, or 17/45 total reports) at multiuse trail-road intersections compared to road-road intersections (23%, or 62/268 total reports). A higher proportion of incidents resulted in an injury at multiuse trail-road intersections compared to road-road intersections (33% versus 15%). Cycling volumes, vehicle volumes, and trail sight distance were all associated with incident frequency at multiuse trail-road intersections. Supplementing traditional crash records with crowdsourced cycling incident data provides valuable evidence on cycling safety at intersections between multiuse trails and roads, and more generally, when conflicts occur between diverse transportation modes. Copyright © 2017. Published by Elsevier Ltd.

  3. Atmospheric motion investigation for vapor trails and radio meteors

    NASA Technical Reports Server (NTRS)

    Bedinger, J.

    1973-01-01

    The dynamics are investigated of the lower thermosphere through comparison of optical observations of motions of ejected vapor trails with radar observations of motions of ionized meteor trails. In particular, the winds obtained from a series of vapor trail observations which occurred at Wallops Island, Virginia during the night of 14-15 December 1970 are to be compared with wind data deduced from radar observations of meteor trails during the same period. The comparison of these data is considered important for two reasons. First, the most widely used methods of measuring winds in the lower thermosphere are the vapor trails and the radar meteors. However, the two techniques differ markedly and the resultant sets of data have been analyzed and presented in different formats. Secondly, and possibly of greater immediate concern is the fact that the radar meteor method appears to be an appropriate approach to the synoptic measurement of winds. During the night of 14-15 December 1970, five vapor trails were ejected from Nike Apache rockets over Wallops Island, Virginia from 2208 EST through 0627 EST. The wind data which were obtained from these trails are presented, and features of the wind profiles which relate to the radar meteor trails results are discussed.

  4. 30 CFR 77.603 - Clamping of trailing cables to equipment.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Clamping of trailing cables to equipment. 77.603 Section 77.603 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR COAL... UNDERGROUND COAL MINES Trailing Cables § 77.603 Clamping of trailing cables to equipment. Trailing cables...

  5. Trailing Vortex-Induced Loads During Close Encounters in Cruise

    NASA Technical Reports Server (NTRS)

    Mendenhall, Michael R.; Lesieutre, Daniel J; Kelly, Michael J.

    2015-01-01

    The trailing vortex induced aerodynamic loads on a Falcon 20G business jet flying in the wake of a DC-8 are predicted to provide a preflight estimate of safe trail distances during flight test measurements in the wake. Static and dynamic loads on the airframe flying in the near wake are shown at a matrix of locations, and the dynamic motion of the Falcon 20G during traverses of the DC-8 primary trailing vortex is simulated. Safe trailing distances for the test flights are determined, and optimum vortex traverse schemes are identified to moderate the motion of the trailing aircraft during close encounters with the vortex wake.

  6. Appalachian National Scenic Trail pilot survey

    Treesearch

    Stan Zarnoch; Michael Bowker; Ken Cordell; Matt Owens; Gary T. Green; Allison Ginn

    2011-01-01

    Visitation statistics on the Appalachian National Scenic Trail (AT) are important for management and Federal Government reporting purposes. However, no survey methodology has been developed to obtain accurate trailwide estimates over linear trails that traverse many hundreds of back-country miles. This research develops a stratified random survey design which utilizes...

  7. Making Sense of Audit Trail Data

    ERIC Educational Resources Information Center

    Kennedy, Gregor E.; Judd, Terry S.

    2004-01-01

    In this paper we argue that the use of audit trail data for research and evaluation purposes has attracted scepticism due to real and perceived difficulties associated with the data's interpretation. We suggest that educational technology researchers and evaluators need to better understand how audit trail data can be processed and analysed…

  8. Trail resource impacts and an examination of alternative assessment techniques

    USGS Publications Warehouse

    Marion, J.L.; Leung, Y.-F.

    2001-01-01

    Trails are a primary recreation resource facility on which recreation activities are performed. They provide safe access to non-roaded areas, support recreational opportunities such as hiking, biking, and wildlife observation, and protect natural resources by concentrating visitor traffic on resistant treads. However, increasing recreational use, coupled with poorly designed and/or maintained trails, has led to a variety of resource impacts. Trail managers require objective information on trails and their conditions to monitor trends, direct trail maintenance efforts, and evaluate the need for visitor management and resource protection actions. This paper reviews trail impacts and different types of trail assessments, including inventory, maintenance, and condition assessment approaches. Two assessment methods, point sampling and problem assessment, are compared empirically from separate assessments of a 15-mile segment of the Appalachian Trail in Great Smoky Mountains National Park. Results indicate that point sampling and problem assessment methods yield distinctly different types of quantitative information. The point sampling method provides more accurate and precise measures of trail characteristics that are continuous or frequent (e.g., tread width or exposed soil). The problem assessment method is a preferred approach for monitoring trail characteristics that can be easily predefined or are infrequent (e.g., excessive width or secondary treads), particularly when information on the location of specific trail impact problems is needed. The advantages and limitations of these two assessment methods are examined in relation to various management and research information needs. The choice and utility of these assessment methods are also discussed.

  9. Analyzing the impact of the Firefly Trail on economic development in northeast Georgia : final report.

    DOT National Transportation Integrated Search

    2016-10-01

    This research report contains the findings of the analysis undertaken to measure the economic impact of the proposed Firefly Trail on the local economy. An input-output model was constructed to study the economic impact of the project on the local ec...

  10. Station-Keeping Strategies for Lead-Trail Formation Flying

    NASA Astrophysics Data System (ADS)

    Martinot, V.; Rozanes, P.

    Numerous projects in the Science and Observation domains involve the use of formation flying to ensure the mission performance. The formation flying configurations proposed in some of them are quite complex with several satellites in different planes generating relative differential motions between the satellites like in case of circular projected formation-flying. However, more simple designs consisting of two satellites in a lead-trail formation appear to be sufficient for a wide range of applications (interferometry, geodesy,...). This article concentrates on the station- keeping phase of such formations in Low-Earth Orbits The station-keeping criterion for such formations can be expressed for example in terms of difference in argument of latitude between both satellites and at the altitudes considered, it evolves mainly under the differential effect of the atmospheric drag between the trailing and leading satellites. In the present paper, this differential effect is supposed to originate from the difference in the area-to-mass ratio between the satellites due to their different designs. A preliminary estimation of the navigation performance is first given assuming that on-board GPS receiver are mounted on each satellite of the formation to acquire pseudo-range measurements between the LEO satellites and the MEO GPS constellation. The distance between both satellites of the formation is derived from independent orbit restitution performed for each LEO satellite in a ground master control station processing the GPS measurements. A strategy for controlling the satellite formation disturbed by the differential effect of the drag is then proposed. Simulations are performed to assess the feasibility of the station-keeping with different types of engines. As by-products, the propellant budget and the frequency of the station-keeping manoeuvres are also given. A case study inspired from the ESA project Acechem/Metop is used for the simulations.

  11. Walking through time: heritage resources within the Appalachian Trail corridor

    Treesearch

    David M. Lacy; Karl Roenke

    1998-01-01

    Parts of the Appalachian Trail (and nearly half of Vermont's Long Trail) are located on New England National Forests, and managed in partnership with the Appalachian Trail Conference, Green Mountain Club, and other volunteers. Since the Trail corridor is a linear, if serpentine, sample of northern Appalachian highland environments it's not surprising that it...

  12. 36 CFR 261.20 - Pacific Crest National Scenic Trail.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Trail. 261.20 Section 261.20 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE PROHIBITIONS General Prohibitions § 261.20 Pacific Crest National Scenic Trail. It is prohibited to use a motorized vehicle on the Pacific Crest National Scenic Trail without a special-use...

  13. Nature Trails for the Visually Impaired.

    ERIC Educational Resources Information Center

    Schwartz, Jonathan R.

    Many interpretive nature trails have been established for the visually impaired in recent years. The objectives of the investigation were to (a) identify what has been done in the past in the way of nature trail design for the visually impaired, (b) compare this with what professional workers for the visually impaired consider important in the…

  14. Triptolide Enhances the Tumoricidal Activity of TRAIL Against Renal Cell Carcinoma

    PubMed Central

    James, Britnie R.; Murphy, Katherine A.; Schwertfeger, Kathryn L.; Sangwan, Veena; Banerjee, Sulagna; Saluja, Ashok K.; Griffith, Thomas S.

    2015-01-01

    Renal cell carcinoma (RCC) is resistant to traditional cancer therapies, and metastatic RCC (mRCC) is incurable. The shortcomings in current therapeutic options for patients with mRCC provide the rationale for the development of novel treatment protocols. TNF-related apoptosis-inducing ligand (TRAIL) has proven to be a potent inducer of tumor cell death in vitro and in vivo, and a number of TRAIL death receptor agonists (recombinant TRAIL or TRAIL death receptor-specific mAb) has been developed and tested clinically. Unfortunately the clinical efficacy of TRAIL has been underwhelming and is likely due to a number of possible mechanisms that render tumors resistant to TRAIL, prompting the search for drugs that increase tumor cell susceptibility to TRAIL. The objective of this study was to determine the effectiveness of combining the diterpene triepoxide triptolide, or its water-soluble prodrug, Minnelide, with TRAIL receptor agonists against RCC in vitro or in vivo, respectively. TRAIL-induced apoptotic death of human RCC cells was increased in the presence of triptolide. The triptolide-induced sensitization was accompanied by increased TRAIL-R2 (DR5) and decreased HSP70 expression. In vivo treatment of mice bearing orthotopic RCC (Renca) tumors showed the combination of Minnelide and agonistic anti-DR5 mAb significantly decreased tumor burden and increased animal survival compared to either therapy alone. Our data suggest triptolide/Minnelide sensitizes RCC cells to TRAIL-induced apoptosis through altered TRAIL death receptor and heat shock protein expression. PMID:26426449

  15. Electronic Escape Trails for Firefighters

    NASA Technical Reports Server (NTRS)

    Jorgensen, Charles; Schipper, John; Betts, Bradley

    2008-01-01

    A proposed wireless-communication and data-processing system would exploit recent advances in radio-frequency identification devices (RFIDs) and software to establish information lifelines between firefighters in a burning building and a fire chief at a control station near but outside the building. The system would enable identification of trails that firefighters and others could follow to escape from the building, including identification of new trails should previously established trails become blocked. The system would include a transceiver unit and a computer at the control station, portable transceiver units carried by the firefighters in the building, and RFID tags that the firefighters would place at multiple locations as they move into and through the building (see figure). Each RFID tag, having a size of the order of a few centimeters, would include at least standard RFID circuitry and possibly sensors for measuring such other relevant environmental parameters as temperature, levels of light and sound, concentration of oxygen, concentrations of hazardous chemicals in smoke, and/or levels of nuclear radiation. The RFID tags would be activated and interrogated by the firefighters and control-station transceivers. Preferably, RFID tags would be configured to communicate with each other and with the firefighters units and the control station in an ordered sequence, with built-in redundancy. In a typical scenario, as firefighters moved through a building, they would scatter many RFID tags into smoke-obscured areas by use of a compressed-air gun. Alternatively or in addition, they would mark escape trails by dropping RFID tags at such points of interest as mantraps, hot spots, and trail waypoints. The RFID tags could be of different types, operating at different frequencies to identify their functions, and possibly responding by emitting audible beeps when activated by signals transmitted by transceiver units carried by nearby firefighters.

  16. 30 CFR 57.12039 - Protection of surplus trailing cables.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Protection of surplus trailing cables. 57.12039 Section 57.12039 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR METAL AND... Electricity Surface and Underground § 57.12039 Protection of surplus trailing cables. Surplus trailing cables...

  17. 30 CFR 75.833 - Handling high-voltage trailing cables.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Handling high-voltage trailing cables. 75.833... High-Voltage Longwalls § 75.833 Handling high-voltage trailing cables. (a) Cable handling. (1) Miners must not handle energized trailing cables unless they are wearing high-voltage insulating gloves, which...

  18. Getting TRAIL back on track for cancer therapy

    PubMed Central

    Lemke, J; von Karstedt, S; Zinngrebe, J; Walczak, H

    2014-01-01

    Unlike other members of the TNF superfamily, the TNF-related apoptosis-inducing ligand (TRAIL, also known as Apo2L) possesses the unique capacity to induce apoptosis selectively in cancer cells in vitro and in vivo. This exciting discovery provided the basis for the development of TRAIL-receptor agonists (TRAs), which have demonstrated robust anticancer activity in a number of preclinical studies. Subsequently initiated clinical trials testing TRAs demonstrated, on the one hand, broad tolerability but revealed, on the other, that therapeutic benefit was rather limited. Several factors that are likely to account for TRAs' sobering clinical performance have since been identified. First, because of initial concerns over potential hepatotoxicity, TRAs with relatively weak agonistic activity were selected to enter clinical trials. Second, although TRAIL can induce apoptosis in several cancer cell lines, it has now emerged that many others, and importantly, most primary cancer cells are resistant to TRAIL monotherapy. Third, so far patients enrolled in TRA-employing clinical trials were not selected for likelihood of benefitting from a TRA-comprising therapy on the basis of a valid(ated) biomarker. This review summarizes and discusses the results achieved so far in TRA-employing clinical trials in the light of these three shortcomings. By integrating recent insight on apoptotic and non-apoptotic TRAIL signaling in cancer cells, we propose approaches to introduce novel, revised TRAIL-based therapeutic concepts into the cancer clinic. These include (i) the use of recently developed highly active TRAs, (ii) the addition of efficient, but cancer-cell-selective TRAIL-sensitizing agents to overcome TRAIL resistance and (iii) employing proteomic profiling to uncover resistance mechanisms. We envisage that this shall enable the design of effective TRA-comprising therapeutic concepts for individual cancer patients in the future. PMID:24948009

  19. Photo-excitable hybrid nanocomposites for image-guided photo/TRAIL synergistic cancer therapy.

    PubMed

    Lin, Gan; Zhang, Yang; Zhu, Congqing; Chu, Chengchao; Shi, Yesi; Pang, Xin; Ren, En; Wu, Yayun; Mi, Peng; Xia, Haiping; Chen, Xiaoyuan; Liu, Gang

    2018-05-22

    Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) can induce apoptosis in cancer cells without toxicity to normal cells. However, the efficiency is greatly limited by its short half-life and wild resistance in various cancer cells. In this study, we reported a micellar hybrid nanoparticle to carry TRAIL ligand (denoted as IPN@TRAIL) for a novel photo-excited TRAIL therapy. These IPN@TRAIL offered increased TRAIL stability, prolonged half-life and enhanced tumor accumulation, monitored by dual mode imaging. Furthermore, IPN@TRAIL nanocomposites enhanced wrapped TRAIL therapeutic efficiency greatly towards resistant cancer cells by TRAIL nanovectorization. More importantly, when upon external laser, these nanocomposites not only triggered tumor photothermal therapy (PTT), but also upregulated the expression of death receptors (DR4 and DR5), resulting in a greater apoptosis mediated by co-delivered TRAIL ligand. Such photo/TRAIL synergistic effect showed its great killing effects in a controllable manner on TRAIL-resistant A549 tumor model bearing mice. Finally, these nanocomposites exhibited rapid clearance without obvious systemic toxicity. All these features rendered our nanocomposites a promising theranostic platform in cancer therapy. Copyright © 2018 Elsevier Ltd. All rights reserved.

  20. Flow monitoring along the western Tamiami Trail between County Road 92 and State Road 29 in support of the Comprehensive Everglades Restoration Plan, 2007-2010

    USGS Publications Warehouse

    Booth, Amanda C.; Soderqvist, Lars E.; Berry, Marcia C.

    2014-01-01

    The construction of U.S. Highway 41 (Tamiami Trail), the Southern Golden Gate Estates development, and the Barron River Canal has altered the flow of freshwater to the Ten Thousand Islands estuary of Southwest Florida. Two restoration projects, the Picayune Strand Restoration Project and the Tamiami Trail Culverts Project, both associated with the Comprehensive Everglades Restoration Plan, were initiated to address this issue. Quantifying the flow of freshwater to the estuary is essential to assessing the effectiveness of these projects. The U.S. Geological Survey conducted a study between March 2006 and September 2010 to quantify the freshwater flowing under theTamiami Trail between County Road 92 and State Road 29 in southwest Florida, excluding the Faka Union Canal (which is monitored by South Florida Water Management District). The study period was after the completion of the Tamiami Trail Culverts Project and prior to most of the construction related to the Picayune Restoration Project. The section of the Tamiami Trail that was studied contains too many structures (35 bridges and 16 culverts) to cost-effectively measure each structure on a continuous basis, so the area was divided into seven subbasins. One bridge within each of the subbasins was instrumented with an acoustic Doppler velocity meter. The index velocity method was used to compute discharge at the seven instrumented bridges. Periodic discharge measurements were made at all structures, using acoustic Doppler current profilers at bridges and acoustic Doppler velocity meters at culverts. Continuous daily mean values of discharge for the uninstrumented structures were calculated on the basis of relations between the measured discharge at the uninstrumented stations and the discharge and stage at the instrumented bridge. Estimates of daily mean discharge are available beginning in 2006 or 2007 through September 2010 for all structures. Subbasin comparison is limited to water years 2008–2010. The Faka

  1. Glucose Deprivation Induces ATF4-Mediated Apoptosis through TRAIL Death Receptors

    PubMed Central

    Iurlaro, Raffaella; Püschel, Franziska; León-Annicchiarico, Clara Lucía; O'Connor, Hazel; Martin, Seamus J.; Palou-Gramón, Daniel; Lucendo, Estefanía

    2017-01-01

    ABSTRACT Metabolic stress occurs frequently in tumors and in normal tissues undergoing transient ischemia. Nutrient deprivation triggers, among many potential cell death-inducing pathways, an endoplasmic reticulum (ER) stress response with the induction of the integrated stress response transcription factor ATF4. However, how this results in cell death remains unknown. Here we show that glucose deprivation triggered ER stress and induced the unfolded protein response transcription factors ATF4 and CHOP. This was associated with the nontranscriptional accumulation of TRAIL receptor 1 (TRAIL-R1) (DR4) and with the ATF4-mediated, CHOP-independent induction of TRAIL-R2 (DR5), suggesting that cell death in this context may involve death receptor signaling. Consistent with this, the ablation of TRAIL-R1, TRAIL-R2, FADD, Bid, and caspase-8 attenuated cell death, although the downregulation of TRAIL did not, suggesting ligand-independent activation of TRAIL receptors. These data indicate that stress triggered by glucose deprivation promotes the ATF4-dependent upregulation of TRAIL-R2/DR5 and TRAIL receptor-mediated cell death. PMID:28242652

  2. Rails-to-Trails: A Valuable Resource for Outdoor Educators.

    ERIC Educational Resources Information Center

    Fischer, Jim

    1993-01-01

    Describes the rails-to-trails movement, particularly in Ontario, where abandoned railways are converted to trails for hiking, skiing, cycling, and horseback riding. Proposes the often controversial rails-to-trails issue as a resource for discussion in outdoor leadership classes, focusing on rural and urban viewpoints. Also suggests using the…

  3. 30 CFR 75.810 - High-voltage trailing cables; splices.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false High-voltage trailing cables; splices. 75.810... § 75.810 High-voltage trailing cables; splices. [Statutory Provisions] In the case of high-voltage cables used as trailing cables, temporary splices shall not be used and all permanent splices shall be...

  4. 30 CFR 18.35 - Portable (trailing) cables and cords.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Portable (trailing) cables and cords. 18.35... and Design Requirements § 18.35 Portable (trailing) cables and cords. (a) Portable cables and cords... a portable (trailing) cable shall not exceed 500 feet. Where the method of mining requires the...

  5. On the Trail of Drug-Defying Superbugs

    MedlinePlus

    ... On the Trail of Drug-Defying Superbugs Inside Life Science View All Articles | Inside Life Science Home Page On the Trail of Drug-Defying ... Other Findings About Our Resident Microbes This Inside Life Science article also appears on LiveScience . Learn about related ...

  6. Trailblazing in East Texas: A Progress Report on the Forest Trail Library Consortium's Networking Project.

    ERIC Educational Resources Information Center

    Claer, Joycelyn H.

    This report describes the development of the Forest Trail Library Consortium (FTLC), a network of academic, public, and school libraries in Texas. The growth of FTLC from 4 charter members to its current 16 members is traced, including details about goals and funding. The role of special interest groups (SIGs) is examined, and the goals and…

  7. Triptolide enhances the tumoricidal activity of TRAIL against renal cell carcinoma.

    PubMed

    Brincks, Erik L; Kucaba, Tamara A; James, Britnie R; Murphy, Katherine A; Schwertfeger, Kathryn L; Sangwan, Veena; Banerjee, Sulagna; Saluja, Ashok K; Griffith, Thomas S

    2015-12-01

    Renal cell carcinoma (RCC) is resistant to traditional cancer therapies, and metastatic RCC (mRCC) is incurable. The shortcomings in current therapeutic options for patients with mRCC provide the rationale for the development of novel treatment protocols. Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) has proven to be a potent inducer of tumor cell death in vitro and in vivo, and a number of TRAIL death receptor agonists (recombinant TRAIL or TRAIL death receptor-specific mAb) have been developed and tested clinically. Unfortunately the clinical efficacy of TRAIL has been underwhelming and is likely due to a number of possible mechanisms that render tumors resistant to TRAIL, prompting the search for drugs that increase tumor cell susceptibility to TRAIL. The objective of this study was to determine the effectiveness of combining the diterpene triepoxide triptolide, or its water-soluble prodrug, Minnelide, with TRAIL receptor agonists against RCC in vitro or in vivo, respectively. TRAIL-induced apoptotic death of human RCC cells was increased in the presence of triptolide. The triptolide-induced sensitization was accompanied by increased TRAIL-R2 (DR5) and decreased heat shock protein 70 expression. In vivo treatment of mice bearing orthotopic RCC (Renca) tumors showed the combination of Minnelide and agonistic anti-DR5 mAb significantly decreased tumor burden and increased animal survival compared to either therapy alone. Our data suggest triptolide/Minnelide sensitizes RCC cells to TRAIL-induced apoptosis through altered TRAIL death receptor and heat shock protein expression. © 2015 FEBS.

  8. Trail Pheromone of the Argentine Ant, Linepithema humile (Mayr) (Hymenoptera: Formicidae)

    PubMed Central

    Choe, Dong-Hwan; Villafuerte, David B.; Tsutsui, Neil D.

    2012-01-01

    The Argentine ant (Linepithema humile) is recognized as one of the world's most damaging invasive species. One reason for the ecological dominance of introduced Argentine ant populations is their ability to dominate food and habitat resources through the rapid mobilization and recruitment of thousands of workers. More than 30 years ago, studies showed that (Z)-9-hexadecenal strongly attracted Argentine ant workers in a multi-choice olfactometer, suggesting that (Z)-9-hexadecenal might be the trail pheromone, or a component of a trail pheromone mixture. Since then, numerous studies have considered (Z)-9-hexadecenal as the key component of the Argentine ant trails. Here, we report the first chemical analyses of the trails laid by living Argentine ants and find that (Z)-9-hexadecenal is not present in a detectible quantity. Instead, two iridoids, dolichodial and iridomyrmecin, appear to be the primary chemical constituents of the trails. Laboratory choice tests confirmed that Argentine ants were attracted to artificial trails comprised of these two chemicals significantly more often than control trails. Although (Z)-9-hexadecenal was not detected in natural trails, supplementation of artificial dolichodial+iridomyrmecin trails with an extremely low concentraion of (Z)-9-hexadecenal did increase the efficacy of the trail-following behavior. In stark contrast with previous dogma, our study suggests that dolichodial and iridomyrmecin are major components of the Argentine ant trail pheromone. (Z)-9-hexadecenal may act in an additive manner with these iridoids, but it does not occur in detectable quantities in Argentine ant recruitment trails. PMID:23028739

  9. Bortezomib sensitises TRAIL-resistant HPV-positive head and neck cancer cells to TRAIL through a caspase-dependent, E6-independent mechanism.

    PubMed

    Bullenkamp, J; Raulf, N; Ayaz, B; Walczak, H; Kulms, D; Odell, E; Thavaraj, S; Tavassoli, M

    2014-10-23

    Human papillomavirus (HPV) is causative for a new and increasing form of head and neck squamous cell carcinomas (HNSCCs). Although localised HPV-positive cancers have a favourable response to radio-chemotherapy (RT/CT), the impact of HPV in advanced or metastatic HNSCC remains to be defined and targeted therapeutics need to be tested for cancers resistant to RT/CT. To this end, we investigated the sensitivity of HPV-positive and -negative HNSCC cell lines to TRAIL (tumour necrosis factor-related apoptosis-inducing ligand), which induces tumour cell-specific apoptosis in various cancer types. A clear correlation was observed between HPV positivity and resistance to TRAIL compared with HPV-negative head and neck cancer cell lines. All TRAIL-resistant HPV-positive cell lines tested were sensitised to TRAIL-induced cell death by treatment with bortezomib, a clinically approved proteasome inhibitor. Bortezomib-mediated sensitisation to TRAIL was associated with enhanced activation of caspase-8, -9 and -3, elevated membrane expression levels of TRAIL-R2, cytochrome c release and G2/M arrest. Knockdown of caspase-8 significantly blocked cell death induced by the combination therapy, whereas the BH3-only protein Bid was not required for induction of apoptosis. XIAP depletion increased the sensitivity of both HPV-positive and -negative cells to TRAIL alone or in combination with bortezomib. In contrast, restoration of p53 following E6 knockdown in HPV-positive cells had no effect on their sensitivity to either single or combination therapy, suggesting a p53-independent pathway for the observed response. In summary, bortezomib-mediated proteasome inhibition sensitises previously resistant HPV-positive HNSCC cells to TRAIL-induced cell death through a mechanism involving both the extrinsic and intrinsic pathways of apoptosis. The cooperative effect of these two targeted anticancer agents therefore represents a promising treatment strategy for RT/CT-resistant HPV

  10. In-Trail Procedure (ITP) Algorithm Design

    NASA Technical Reports Server (NTRS)

    Munoz, Cesar A.; Siminiceanu, Radu I.

    2007-01-01

    The primary objective of this document is to provide a detailed description of the In-Trail Procedure (ITP) algorithm, which is part of the Airborne Traffic Situational Awareness In-Trail Procedure (ATSA-ITP) application. To this end, the document presents a high level description of the ITP Algorithm and a prototype implementation of this algorithm in the programming language C.

  11. 30 CFR 77.600 - Trailing cables; short-circuit protection; disconnecting devices.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... capacity in each ungrounded conductor. Disconnecting devices used to disconnect power from trailing cables... 30 Mineral Resources 1 2014-07-01 2014-07-01 false Trailing cables; short-circuit protection... AREAS OF UNDERGROUND COAL MINES Trailing Cables § 77.600 Trailing cables; short-circuit protection...

  12. 30 CFR 77.600 - Trailing cables; short-circuit protection; disconnecting devices.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... capacity in each ungrounded conductor. Disconnecting devices used to disconnect power from trailing cables... 30 Mineral Resources 1 2013-07-01 2013-07-01 false Trailing cables; short-circuit protection... AREAS OF UNDERGROUND COAL MINES Trailing Cables § 77.600 Trailing cables; short-circuit protection...

  13. 30 CFR 77.600 - Trailing cables; short-circuit protection; disconnecting devices.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... capacity in each ungrounded conductor. Disconnecting devices used to disconnect power from trailing cables... 30 Mineral Resources 1 2012-07-01 2012-07-01 false Trailing cables; short-circuit protection... AREAS OF UNDERGROUND COAL MINES Trailing Cables § 77.600 Trailing cables; short-circuit protection...

  14. 30 CFR 77.600 - Trailing cables; short-circuit protection; disconnecting devices.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... capacity in each ungrounded conductor. Disconnecting devices used to disconnect power from trailing cables... 30 Mineral Resources 1 2011-07-01 2011-07-01 false Trailing cables; short-circuit protection... AREAS OF UNDERGROUND COAL MINES Trailing Cables § 77.600 Trailing cables; short-circuit protection...

  15. Experimental evaluation of certification trails using abstract data type validation

    NASA Technical Reports Server (NTRS)

    Wilson, Dwight S.; Sullivan, Gregory F.; Masson, Gerald M.

    1993-01-01

    Certification trails are a recently introduced and promising approach to fault-detection and fault-tolerance. Recent experimental work reveals many cases in which a certification-trail approach allows for significantly faster program execution time than a basic time-redundancy approach. Algorithms for answer-validation of abstract data types allow a certification trail approach to be used for a wide variety of problems. An attempt to assess the performance of algorithms utilizing certification trails on abstract data types is reported. Specifically, this method was applied to the following problems: heapsort, Hullman tree, shortest path, and skyline. Previous results used certification trails specific to a particular problem and implementation. The approach allows certification trails to be localized to 'data structure modules,' making the use of this technique transparent to the user of such modules.

  16. Pheromone trailing behavior of the brown tree snake, Boiga irregularis.

    PubMed

    Greene, M J; Stark, S L; Mason, R T

    2001-11-01

    The ability of snakes to follow pheromone trails has significant consequences for survival and reproduction. Of particular importance is the ability of snakes to locate conspecifics during the breeding season via the detection of pheromone trails. In this study, the ability of male brown tree snakes (Boiga irregularis), a tropical, rear-fanged colubrid, to follow pheromone trails produced by reproductively active conspecifics was tested in the laboratory by using a Y maze. Males displayed a trailing response to both female and male pheromone trails over blank controls. As males of this species display ritualized combat behavior, these responses likely represent both direct and indirect mechanisms, respectively, for the location of potential mates in the wild. Males did not, however, discriminate between male and female trails when given a choice on the Y maze.

  17. Using certification trails to achieve software fault tolerance

    NASA Technical Reports Server (NTRS)

    Sullivan, Gregory F.; Masson, Gerald M.

    1993-01-01

    A conceptually novel and powerful technique to achieve fault tolerance in hardware and software systems is introduced. When used for software fault tolerance, this new technique uses time and software redundancy and can be outlined as follows. In the initial phase, a program is run to solve a problem and store the result. In addition, this program leaves behind a trail of data called a certification trail. In the second phase, another program is run which solves the original problem again. This program, however, has access to the certification trail left by the first program. Because of the availability of the certification trail, the second phase can be performed by a less complex program and can execute more quickly. In the final phase, the two results are accepted as correct; otherwise an error is indicated. An essential aspect of this approach is that the second program must always generate either an error indication or a correct output even when the certification trail it receives from the first program is incorrect. The certification trail approach to fault tolerance was formalized and it was illustrated by applying it to the fundamental problem of finding a minimum spanning tree. Cases in which the second phase can be run concorrectly with the first and act as a monitor are discussed. The certification trail approach was compared to other approaches to fault tolerance. Because of space limitations we have omitted examples of our technique applied to the Huffman tree, and convex hull problems. These can be found in the full version of this paper.

  18. Reducing HIV infection among new injecting drug users in the China-Vietnam Cross Border Project.

    PubMed

    Des Jarlais, Don C; Kling, Ryan; Hammett, Theodore M; Ngu, Doan; Liu, Wei; Chen, Yi; Binh, Kieu Thanh; Friedmann, Patricia

    2007-12-01

    To assess an HIV prevention programme for injecting drug users (IDU) in the crossborder area between China and Vietnam. Serial cross-sectional surveys (0, 6, 12, 18, 24 and 36 months) of community-recruited current IDU. The project included peer educator outreach and the large-scale distribution of sterile injection equipment. Serial cross-sectional surveys with HIV testing of community recruited IDU were conducted at baseline (before implementation) and 6, 12, 18, 24 and 36 months post-baseline. HIV prevalence and estimated HIV incidence among new injectors (individuals injecting drugs for < 3 years) in each survey wave were the primary outcome measures. The percentages of new injectors among all subjects declined across each survey waves in both Ning Ming and Lang Son. HIV prevalence and estimated incidence fell by approximately half at the 24-month survey and by approximately three quarters at the 36-month survey in both areas (all P < 0.01). The implementation of large-scale outreach and syringe access programmes was followed by substantial reductions in HIV infection among new injectors, with no evidence of any increase in individuals beginning to inject drugs. This project may serve as a model for large-scale HIV prevention programming for IDU in China, Vietnam, and other developing/transitional countries.

  19. 77 FR 25910 - National Trails System Act and Railroad Rights-of-Way

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-02

    ...] National Trails System Act and Railroad Rights-of-Way AGENCY: Surface Transportation Board, DOT. ACTION...) for rail banking and interim trail use under the National Trails System Act (Trails Act). New rules are adopted that require the parties jointly to notify the Board when an interim trail use/rail...

  20. Snail Trails

    ERIC Educational Resources Information Center

    Galus, Pamela

    2002-01-01

    The slime trails of snails lead the author's students to a better understanding of science as inquiry and the processes of science. During this five-day activity, students get up close and personal with one of her favorite creatures, the land snail. Students begin by observing the organism and recording their observations. After making initial…

  1. 49 CFR 236.823 - Switch, trailing point.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false Switch, trailing point. 236.823 Section 236.823 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL RAILROAD ADMINISTRATION... Switch, trailing point. A switch, the points of which face away from traffic approaching in the direction...

  2. 49 CFR 236.823 - Switch, trailing point.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 49 Transportation 4 2011-10-01 2011-10-01 false Switch, trailing point. 236.823 Section 236.823 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL RAILROAD ADMINISTRATION... Switch, trailing point. A switch, the points of which face away from traffic approaching in the direction...

  3. 49 CFR 236.823 - Switch, trailing point.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 49 Transportation 4 2012-10-01 2012-10-01 false Switch, trailing point. 236.823 Section 236.823 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL RAILROAD ADMINISTRATION... Switch, trailing point. A switch, the points of which face away from traffic approaching in the direction...

  4. 49 CFR 236.823 - Switch, trailing point.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 49 Transportation 4 2013-10-01 2013-10-01 false Switch, trailing point. 236.823 Section 236.823 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL RAILROAD ADMINISTRATION... Switch, trailing point. A switch, the points of which face away from traffic approaching in the direction...

  5. 49 CFR 236.823 - Switch, trailing point.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 49 Transportation 4 2014-10-01 2014-10-01 false Switch, trailing point. 236.823 Section 236.823 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL RAILROAD ADMINISTRATION... Switch, trailing point. A switch, the points of which face away from traffic approaching in the direction...

  6. Fast and flexible: argentine ants recruit from nearby trails.

    PubMed

    Flanagan, Tatiana P; Pinter-Wollman, Noa M; Moses, Melanie E; Gordon, Deborah M

    2013-01-01

    Argentine ants (Linepithema humile) live in groups of nests connected by trails to each other and to stable food sources. In a field study, we investigated whether some ants recruit directly from established, persistent trails to food sources, thus accelerating food collection. Our results indicate that Argentine ants recruit nestmates to food directly from persistent trails, and that the exponential increase in the arrival rate of ants at baits is faster than would be possible if recruited ants traveled from distant nests. Once ants find a new food source, they walk back and forth between the bait and sometimes share food by trophallaxis with nestmates on the trail. Recruiting ants from nearby persistent trails creates a dynamic circuit, like those found in other distributed systems, which facilitates a quick response to changes in available resources.

  7. 76 FR 35468 - Star-Spangled Banner National Historic Trail Advisory Council

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-17

    ... DEPARTMENT OF THE INTERIOR National Park Service Star-Spangled Banner National Historic Trail... the Advisory Committee on the Star-Spangled Banner National Historic Trail will hold a meeting. Designated through an amendment to the National Trails System Act (16 U.S.C. 1241), the trail consists of...

  8. 36 CFR 212.56 - Identification of designated roads, trails, and areas.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... roads, trails, and areas. 212.56 Section 212.56 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE TRAVEL MANAGEMENT Designation of Roads, Trails, and Areas for Motor Vehicle Use § 212.56 Identification of designated roads, trails, and areas. Designated roads, trails, and areas...

  9. 30 CFR 75.607 - Breaking trailing cable and power cable connections.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Breaking trailing cable and power cable... LABOR COAL MINE SAFETY AND HEALTH MANDATORY SAFETY STANDARDS-UNDERGROUND COAL MINES Trailing Cables § 75.607 Breaking trailing cable and power cable connections. [Statutory Provisions] Trailing cable and...

  10. 30 CFR 77.600 - Trailing cables; short-circuit protection; disconnecting devices.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Trailing cables; short-circuit protection... AREAS OF UNDERGROUND COAL MINES Trailing Cables § 77.600 Trailing cables; short-circuit protection; disconnecting devices. Short-circuit protection for trailing cables shall be provided by an automatic circuit...

  11. A spatial exploration of informal trail networks within Great Falls Park, VA

    USGS Publications Warehouse

    Wimpey, Jeremy; Marion, Jeffrey L.

    2011-01-01

    Informal (visitor-created) trails represent a threat to the natural resources of protected natural areas around the globe. These trails can remove vegetation, displace wildlife, alter hydrology, alter habitat, spread invasive species, and fragment landscapes. This study examines informal and formal trails within Great Falls Park, VA, a sub-unit of the George Washington Memorial Parkway, managed by the U.S. National Park Service. This study sought to answer three specific questions: 1) Are the physical characteristics and topographic alignments of informal trails significantly different from formal trails, 2) Can landscape fragmentation metrics be used to summarize the relative impacts of formal and informal trail networks on a protected natural area? and 3) What can we learn from examining the spatial distribution of the informal trails within protected natural areas? Statistical comparisons between formal and informal trails in this park indicate that informal trails have less sustainable topographic alignments than their formal counterparts. Spatial summaries of the lineal and areal extent and fragmentation associated with the trail networks by park management zones compare park management goals to the assessed attributes. Hot spot analyses highlight areas of high trail density within the park and findings provide insights regarding potential causes for development of dense informal trail networks.

  12. Alternative skid trail retirement options for steep terrain logging

    Treesearch

    Mathew F. Smidt; Randall K. Kolka

    2001-01-01

    In winter 1999-2000 trials of deep tillage and recontouring of skid trails were implemented on three sites in northeastern Kentucky, USA to examine their potential as skid trail retirement options. While effective, current Best Management Practices (BMPs) for trail retirement do not address two potential benefits of retirement: recovery of normal hill slope hydrology...

  13. Alternative skid trail retirement options for steep terrain logging

    Treesearch

    Mathew F. Smidt; Randall K. Kelka

    2001-01-01

    In winter 1999-2000 trials of deep tillage and recontouring of skid trails were implemented on three sites in northeastern Kentucky, USA to examine their potential as skid trail retirement options. While effective, current Best Management Practices (BMPs) for trail retirement do not address two potential benefits of retirement: recovery of normal hill slope\\r\\...

  14. 30 CFR 77.605 - Breaking trailing cable and power cable connections.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Breaking trailing cable and power cable... OF UNDERGROUND COAL MINES Trailing Cables § 77.605 Breaking trailing cable and power cable connections. Trailing cable and power cable connections between cables and to power sources shall not be made...

  15. 30 CFR 77.601 - Trailing cables or portable cables; temporary splices.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Trailing cables or portable cables; temporary... OF UNDERGROUND COAL MINES Trailing Cables § 77.601 Trailing cables or portable cables; temporary splices. Temporary splices in trailing cables or portable cables shall be made in a workmanlike manner and...

  16. 30 CFR 77.605 - Breaking trailing cable and power cable connections.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 30 Mineral Resources 1 2013-07-01 2013-07-01 false Breaking trailing cable and power cable... OF UNDERGROUND COAL MINES Trailing Cables § 77.605 Breaking trailing cable and power cable connections. Trailing cable and power cable connections between cables and to power sources shall not be made...

  17. 30 CFR 77.605 - Breaking trailing cable and power cable connections.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 30 Mineral Resources 1 2011-07-01 2011-07-01 false Breaking trailing cable and power cable... OF UNDERGROUND COAL MINES Trailing Cables § 77.605 Breaking trailing cable and power cable connections. Trailing cable and power cable connections between cables and to power sources shall not be made...

  18. 30 CFR 77.605 - Breaking trailing cable and power cable connections.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 30 Mineral Resources 1 2012-07-01 2012-07-01 false Breaking trailing cable and power cable... OF UNDERGROUND COAL MINES Trailing Cables § 77.605 Breaking trailing cable and power cable connections. Trailing cable and power cable connections between cables and to power sources shall not be made...

  19. 30 CFR 77.605 - Breaking trailing cable and power cable connections.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 30 Mineral Resources 1 2014-07-01 2014-07-01 false Breaking trailing cable and power cable... OF UNDERGROUND COAL MINES Trailing Cables § 77.605 Breaking trailing cable and power cable connections. Trailing cable and power cable connections between cables and to power sources shall not be made...

  20. Population dynamics of American dog ticks (Acari: Ixodidae) along park trails

    USGS Publications Warehouse

    Carroll, J.F.; Russek-Cohen, E.; Nichols, J.D.; Hines, J.E.

    1991-01-01

    We conclude a mark-recapture study in which drag-collected ticks were removed from some park trails weekly from April to July. Weekly survival rates (probability of surviving and remaining on the trails) were significantly lower on trials used heavily by hikers, horses, and pets than on trails used less frequently. Although usage was the only obvious difference among these trails, differences in weekly survival rate estimates may be attributable to differential success in acquiring hosts. The estimated probability of capturing a host-seeking tick located along a trail on a single drag was 0.20 on the drag alone, and 0.25 including the person dragging. When routes parallel to the trails and of equal lengths were dragged immediately after sampling the trails, only .apprxeq. 5% as many ticks (including ticks on the person dragging) were found off the trails as on them. We found no evidence of reduced tick numbers on removal trails, but this result should be considered inconclusive because the power of the discerning test was low. However, the data reported here provide insights into turnover rates of the adult Dermacentor variabilis population and effectiveness of the drag as a sampling device.

  1. The application of laser Doppler velocimetry to trailing vortex definition and alleviation

    NASA Technical Reports Server (NTRS)

    Orloff, K. L.; Grant, G. R.

    1973-01-01

    A laser Doppler velocimeter whose focal volume can be rapidly traversed through a flowfield has been used to overcome the problem introduced by excursions of the central vortex filament within a wind tunnel test section. The basic concepts of operation of the instrument are reviewed and data are presented which accurately define the trailing vortex from a square-tipped rectangular wing. Measured axial and tangential velocity distributions are given, both with and without a vortex dissipator panel installed at the wing tip. From the experimental data, circulation and vorticity distributions are obtained and the effect of turbulence injection into the vortex structure is discussed.

  2. Certification trails and software design for testability

    NASA Technical Reports Server (NTRS)

    Sullivan, Gregory F.; Wilson, Dwight S.; Masson, Gerald M.

    1993-01-01

    Design techniques which may be applied to make program testing easier were investigated. Methods for modifying a program to generate additional data which we refer to as a certification trail are presented. This additional data is designed to allow the program output to be checked more quickly and effectively. Certification trails were described primarily from a theoretical perspective. A comprehensive attempt to assess experimentally the performance and overall value of the certification trail method is reported. The method was applied to nine fundamental, well-known algorithms for the following problems: convex hull, sorting, huffman tree, shortest path, closest pair, line segment intersection, longest increasing subsequence, skyline, and voronoi diagram. Run-time performance data for each of these problems is given, and selected problems are described in more detail. Our results indicate that there are many cases in which certification trails allow for significantly faster overall program execution time than a 2-version programming approach, and also give further evidence of the breadth of applicability of this method.

  3. Miniature Trailing Edge Effector for Aerodynamic Control

    NASA Technical Reports Server (NTRS)

    Lee, Hak-Tae (Inventor); Bieniawski, Stefan R. (Inventor); Kroo, Ilan M. (Inventor)

    2008-01-01

    Improved miniature trailing edge effectors for aerodynamic control are provided. Three types of devices having aerodynamic housings integrated to the trailing edge of an aerodynamic shape are presented, which vary in details of how the control surface can move. A bucket type device has a control surface which is the back part of a C-shaped member having two arms connected by the back section. The C-shaped section is attached to a housing at the ends of the arms, and is rotatable about an axis parallel to the wing trailing edge to provide up, down and neutral states. A flip-up type device has a control surface which rotates about an axis parallel to the wing trailing edge to provide up, down, neutral and brake states. A rotating type device has a control surface which rotates about an axis parallel to the chord line to provide up, down and neutral states.

  4. Anticancer Efficacy of Apo2L/TRAIL Is Retained in the Presence of High and Biologically Active Concentrations of Osteoprotegerin In Vivo

    PubMed Central

    Zinonos, Irene; Labrinidis, Agatha; Lee, Michelle; Liapis, Vasilios; Hay, Shelley; Ponomarev, Vladimir; Diamond, Peter; Findlay, David M; Zannettino, Andrew CW; Evdokiou, Andreas

    2017-01-01

    Osteoprotegerin (OPG) is a secreted member of the tumor necrosis factor (TNF) receptor superfamily that binds to the ligand for receptor activator of nuclear factor κB (RANKL) and inhibits bone resorption. OPG can also bind and inhibit the activity of the TNF-related apoptosis-inducing ligand (Apo2L/TRAIL), raising the possibility that the anticancer efficacy of soluble Apo2L/TRAIL may be abrogated in the bone microenvironment where OPG expression is high. In this study we used a murine model of breast cancer growth in bone to evaluate the efficacy of recombinant soluble Apo2L/TRAIL against intratibial tumors that were engineered to overexpress native full-length human OPG. In vitro, OPG-overexpressing breast cancer cells were protected from Apo2L/TRAIL-induced apoptosis, an effect that was reversed with the addition of soluble RANKL or neutralizing antibodies to OPG. In vivo, mice injected intratibially with cells containing the empty vector developed large osteolytic lesions. In contrast, OPG overexpression preserved the integrity of bone and prevented breast cancer–induced bone destruction. This effect was due primarily to the complete absence of osteoclasts in the tibias of mice inoculated with OPG-transfected cells, confirming the biologic activity of the transfected OPG in vivo. Despite the secretion of supraphysiologic levels of OPG, treatment with Apo2L/TRAIL resulted in strong growth inhibition of both empty vector and OPG-overexpressing intratibial tumors. While Apo2L/TRAIL-induced apoptosis may be abrogated in vitro by OPG overexpression, the in vivo anticancer efficacy of recombinant soluble Apo2L/TRAIL is retained in the bone microenvironment even in the presence of biologically active OPG at supraphysiologic concentrations. PMID:20818644

  5. A PIV Study of Slotted Air Injection for Jet Noise Reduction

    NASA Technical Reports Server (NTRS)

    Henderson, Brenda S.; Wernet, Mark P.

    2012-01-01

    Results from acoustic and Particle Image Velocimetry (PIV) measurements are presented for single and dual-stream jets with fluidic injection on the core stream. The fluidic injection nozzles delivered air to the jet through slots on the interior of the nozzle at the nozzle trailing edge. The investigations include subsonic and supersonic jet conditions. Reductions in broadband shock noise and low frequency mixing noise were obtained with the introduction of fluidic injection on single stream jets. Fluidic injection was found to eliminate shock cells, increase jet mixing, and reduce turbulent kinetic energy levels near the end of the potential core. For dual-stream subsonic jets, the introduction of fluidic injection reduced low frequency noise in the peak jet noise direction and enhanced jet mixing. For dual-stream jets with supersonic fan streams and subsonic core streams, the introduction of fluidic injection in the core stream impacted the jet shock cell structure but had little effect on mixing between the core and fan streams.

  6. Fast and Flexible: Argentine Ants Recruit from Nearby Trails

    PubMed Central

    Flanagan, Tatiana P.; Pinter-Wollman, Noa M.; Moses, Melanie E.; Gordon, Deborah M.

    2013-01-01

    Argentine ants (Linepithema humile) live in groups of nests connected by trails to each other and to stable food sources. In a field study, we investigated whether some ants recruit directly from established, persistent trails to food sources, thus accelerating food collection. Our results indicate that Argentine ants recruit nestmates to food directly from persistent trails, and that the exponential increase in the arrival rate of ants at baits is faster than would be possible if recruited ants traveled from distant nests. Once ants find a new food source, they walk back and forth between the bait and sometimes share food by trophallaxis with nestmates on the trail. Recruiting ants from nearby persistent trails creates a dynamic circuit, like those found in other distributed systems, which facilitates a quick response to changes in available resources. PMID:23967129

  7. How Networks of Informal Trails Cause Landscape Level Damage to Vegetation.

    PubMed

    Barros, Agustina; Marina Pickering, Catherine

    2017-07-01

    When visitors are not constrained to remain on formal trails, informal trail networks can develop and damage plant communities in protected areas. These networks can form in areas with low growing vegetation, where formal trails are limited, where there is limited regulation and where vegetation is slow to recover once disturbed. To demonstrate the extent of impacts from unregulated recreational use, we assessed damage to alpine vegetation by hikers and pack animals in the highest protected area in the southern Hemisphere: Aconcagua Park, in the Andes. Within the 237 ha area surveyed in the Horcones Valley, over 19 km of trails were found, nearly all of which (94%) were informal. This network of trails resulted in the direct loss of 11.5 ha of vegetation and extensive fragmentation of alpine meadows (21 fragments) and steppe vegetation (68 fragments). When levels of disturbance off these trails were quantified using rapid visual assessments, 81% of 102 randomly located plots showed evidence of disturbance, with the severity of disturbance greatest close to trails. As a result, vegetation in 90% of the Valley has been damaged by visitor use, nearly all of it from unregulated use. These results highlight the extent to which informal trails and trampling off-trail can cause landscape damage to areas of high conservation value, and hence the importance of better regulation of visitor use. The methodology used for off-trail impact assessment can be easily applied or adapted for other popular protected areas where trampling off-trail is also an issue.

  8. Airbag Trails-2

    NASA Technical Reports Server (NTRS)

    2004-01-01

    This segment of the first color image from the panoramic camera on the Mars Exploration Rover Spirit shows the rover's airbag trails (upper left). These depressions in the soil were made when the airbags were deflated and retracted after landing.

  9. Apigenin promotes TRAIL-mediated apoptosis regardless of ROS generation.

    PubMed

    Kang, Chang-Hee; Molagoda, Ilandarage Menu Neelaka; Choi, Yung Hyun; Park, Cheol; Moon, Dong-Oh; Kim, Gi-Young

    2018-01-01

    Apigenin is a bioactive flavone in several herbs including parsley, thyme, and peppermint. Apigenin possesses anti-cancer and anti-inflammatory properties; however, whether apigenin enhances TRAIL-mediated apoptosis in cancer cells is unknown. In the current study, we found that apigenin enhanced TRAIL-induced apoptosis by promoting caspase activation and death receptor 5 (DR5) expression and a chimeric antibody against DR5 completely blocked the apoptosis. Apigenin also upregulated reactive oxygen species (ROS) generation; however, intriguingly, ROS inhibitors, glutathione (GSH) or N-acetyl-l-cysteine (NAC), moderately increased apigenin/TRAIL-induced apoptosis. Additional results showed that an autophagy inducer, rapamycin, enhanced apigenin/TRAIL-mediated apoptosis by a slight increase of ROS generation. Accordingly, NAC and GSH rather decreased apigenin-induced autophagy formation, suggesting that apigenin-induced ROS generation increased autophagy formation. However, autophagy inhibitors, bafilomycin (BAF) and 3-methyladenine (3-MA), showed different result in apigenin/TRAIL-mediated apoptosis without ROS generation. 3-MA upregulated the apoptosis but remained ROS levels; however, no changes on apoptosis and ROS generation were observed by BAF treatment. Taken together, these findings reveal that apigenin enhances TRAIL-induced apoptosis by activating apoptotic caspases by upregulating DR5 expression regardless of ROS generation, which may be a promising strategy for an adjuvant of TRAIL. Copyright © 2017 Elsevier Ltd. All rights reserved.

  10. The trail guide system as a backcountry management tool

    Treesearch

    Herbert E. Echelberger; Raymond E. Leonard; Marysewall Lindsey Hamblin

    1978-01-01

    A trail guide booklet containing a map, directional and distance data, and information about the natural and human history and management problems of a backcountry hiking trail was keyed to small, numbered, wooden markers along the trail. This system was evaluated on an 8-mile loop in the White Mountain National Forest in New Hampshire. The system may be useful for...

  11. ONC201 Demonstrates Antitumor Effects in Both Triple-Negative and Non-Triple-Negative Breast Cancers through TRAIL-Dependent and TRAIL-Independent Mechanisms.

    PubMed

    Ralff, Marie D; Kline, Christina L B; Küçükkase, Ozan C; Wagner, Jessica; Lim, Bora; Dicker, David T; Prabhu, Varun V; Oster, Wolfgang; El-Deiry, Wafik S

    2017-07-01

    Breast cancer is a major cause of cancer-related death. TNF-related apoptosis-inducing ligand (TRAIL) has been of interest as a cancer therapeutic, but only a subset of triple-negative breast cancers (TNBC) is sensitive to TRAIL. The small-molecule ONC201 induces expression of TRAIL and its receptor DR5. ONC201 has entered clinical trials in advanced cancers. Here, we show that ONC201 is efficacious against both TNBC and non-TNBC cells ( n = 13). A subset of TNBC and non-TNBC cells succumbs to ONC201-induced cell death. In 2 of 8 TNBC cell lines, ONC201 treatment induces caspase-8 cleavage and cell death that is blocked by TRAIL-neutralizing antibody RIK2. The proapoptotic effect of ONC201 translates to in vivo efficacy in the MDA-MB-468 xenograft model. In most TNBC lines tested (6/8), ONC201 has an antiproliferative effect but does not induce apoptosis. ONC201 decreases cyclin D1 expression and causes an accumulation of cells in the G 1 phase of the cell cycle. pRb expression is associated with sensitivity to the antiproliferative effects of ONC201, and the compound synergizes with taxanes in less sensitive cells. All non-TNBC cells ( n = 5) are growth inhibited following ONC201 treatment, and unlike what has been observed with TRAIL, a subset ( n = 2) shows PARP cleavage. In these cells, cell death induced by ONC201 is TRAIL independent. Our data demonstrate that ONC201 has potent antiproliferative and proapoptotic effects in a broad range of breast cancer subtypes, through TRAIL-dependent and TRAIL-independent mechanisms. These findings develop a preclinical rationale for developing ONC201 as a single agent and/or in combination with approved therapies in breast cancer. Mol Cancer Ther; 16(7); 1290-8. ©2017 AACR . ©2017 American Association for Cancer Research.

  12. Back in Time on a Mathematics Trail

    ERIC Educational Resources Information Center

    Moffett, Pamela

    2010-01-01

    The recently revised "Northern Ireland Primary Curriculum" recommends that teachers make use of the environment to extend children's understanding of mathematics. One approach to using the environment in mathematics is to take children on a mathematics trail. A mathematics trail uses the resources and features within the environment as a…

  13. Gingerol sensitizes TRAIL-induced apoptotic cell death of glioblastoma cells

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Lee, Dae-Hee, E-mail: leedneo@gmail.com; Kim, Dong-Wook; Jung, Chang-Hwa

    Glioblastoma multiforme (GBM) is the most lethal and aggressive astrocytoma of primary brain tumors in adults. Although there are many clinical trials to induce the cell death of glioblastoma cells, most glioblastoma cells have been reported to be resistant to TRAIL-induced apoptosis. Here, we showed that gingerol as a major component of ginger can induce TRAIL-mediated apoptosis of glioblastoma. Gingerol increased death receptor (DR) 5 levels in a p53-dependent manner. Furthermore, gingerol decreased the expression level of anti-apoptotic proteins (survivin, c-FLIP, Bcl-2, and XIAP) and increased pro-apoptotic protein, Bax and truncate Bid, by generating reactive oxygen species (ROS). We alsomore » found that the sensitizing effects of gingerol in TRAIL-induced cell death were blocked by scavenging ROS or overexpressing anti-apoptotic protein (Bcl-2). Therefore, we showed the functions of gingerol as a sensitizing agent to induce cell death of TRAIL-resistant glioblastoma cells. This study gives rise to the possibility of applying gingerol as an anti-tumor agent that can be used for the purpose of combination treatment with TRAIL in TRAIL-resistant glioblastoma tumor therapy. - Highlights: • Most GBM cells have been reported to be resistant to TRAIL-induced apoptosis. • Gingerol enhances the expression level of anti-apoptotic proteins by ROS. • Gingerol enhances TRAIL-induced apoptosis through actions on the ROS–Bcl2 pathway.« less

  14. Spectra of Full 3-D PIC Simulations of Finite Meteor Trails

    NASA Astrophysics Data System (ADS)

    Tarnecki, L. K.; Oppenheim, M. M.

    2016-12-01

    Radars detect plasma trails created by the billions of small meteors that impact the Earth's atmosphere daily, returning data used to infer characteristics of the meteoroid population and upper atmosphere. Researchers use models to investigate the dynamic evolution of the trails. Previously, all models assumed a trail of infinite length, due to the constraints of simulation techniques. We present the first simulations of 3D meteor trails of finite length. This change more accurately captures the physics of the trails. We characterize the turbulence that develops as the trail evolves and study the effects of varying the external electric field, altitude, and initial density. The simulations show that turbulence develops in all cases, and that trails travel with the neutral wind rather than electric field. Our results will allow us to draw more detailed and accurate information from non-specular radar observations of meteors.

  15. Human Embryonic and Induced Pluripotent Stem Cells Express TRAIL Receptors and Can Be Sensitized to TRAIL-Induced Apoptosis

    PubMed Central

    Vinarsky, Vladimir; Krivanek, Jan; Rankel, Liina; Nahacka, Zuzana; Barta, Tomas; Jaros, Josef; Andera, Ladislav

    2013-01-01

    Death ligands and their tumor necrosis factor receptor (TNFR) family receptors are the best-characterized and most efficient inducers of apoptotic signaling in somatic cells. In this study, we analyzed whether these prototypic activators of apoptosis are also expressed and able to be activated in human pluripotent stem cells. We examined human embryonic stem cells (hESC) and human-induced pluripotent stem cells (hiPSC) and found that both cell types express primarily TNF-related apoptosis-inducing ligand (TRAIL) receptors and TNFR1, but very low levels of Fas/CD95. We also found that although hESC and hiPSC contain all the proteins required for efficient induction and progression of extrinsic apoptotic signaling, they are resistant to TRAIL-induced apoptosis. However, both hESC and hiPSC can be sensitized to TRAIL-induced apoptosis by co-treatment with protein synthesis inhibitors such as the anti-leukemia drug homoharringtonine (HHT). HHT treatment led to suppression of cellular FLICE inhibitory protein (cFLIP) and Mcl-1 expression and, in combination with TRAIL, enhanced processing of caspase-8 and full activation of caspase-3. cFLIP likely represents an important regulatory node, as its shRNA-mediated down-regulation significantly sensitized hESC to TRAIL-induced apoptosis. Thus, we provide the first evidence that, irrespective of their origin, human pluripotent stem cells express canonical components of the extrinsic apoptotic system and on stress can activate death receptor-mediated apoptosis. PMID:23806100

  16. 30 CFR 57.12088 - Splicing trailing cables.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... cable reel or other power feed cable payout-retrieval system. However, a temporary splice may be made to... 30 Mineral Resources 1 2014-07-01 2014-07-01 false Splicing trailing cables. 57.12088 Section 57... Underground Only § 57.12088 Splicing trailing cables. No splice, except a vulcanized splice or its equivalent...

  17. 30 CFR 57.12088 - Splicing trailing cables.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... cable reel or other power feed cable payout-retrieval system. However, a temporary splice may be made to... 30 Mineral Resources 1 2011-07-01 2011-07-01 false Splicing trailing cables. 57.12088 Section 57... Underground Only § 57.12088 Splicing trailing cables. No splice, except a vulcanized splice or its equivalent...

  18. 30 CFR 57.12088 - Splicing trailing cables.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... cable reel or other power feed cable payout-retrieval system. However, a temporary splice may be made to... 30 Mineral Resources 1 2013-07-01 2013-07-01 false Splicing trailing cables. 57.12088 Section 57... Underground Only § 57.12088 Splicing trailing cables. No splice, except a vulcanized splice or its equivalent...

  19. 30 CFR 57.12088 - Splicing trailing cables.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... cable reel or other power feed cable payout-retrieval system. However, a temporary splice may be made to... 30 Mineral Resources 1 2012-07-01 2012-07-01 false Splicing trailing cables. 57.12088 Section 57... Underground Only § 57.12088 Splicing trailing cables. No splice, except a vulcanized splice or its equivalent...

  20. 77 FR 39733 - Proposed Information Collection; Comment Request: Appalachian Trail Management Partner Survey

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-05

    ... Collection; Comment Request: Appalachian Trail Management Partner Survey AGENCY: National Park Service (NPS... the Appalachian Trail Park Office (ATPO) to protect trail resources and provide for the public enjoyment and visitor experience of the Appalachian National Scenic Trail (Trail). To comply with the...

  1. Mountain bike trail compaction relation to selected physical parameters

    Treesearch

    Jeff Hale; Rodney R. Zwick

    2002-01-01

    The purpose of this research is to explore the rates of compaction and their relation to trail contextual aspects of: soil type, slope and crown cover on a newly established mountain bike trail in the northern reach of Vermont. A random sample of 52 sites was selected for monitoring on the 1.09-mile trail. Three penetrometer readings were taken at each of the sample...

  2. Recreational trails reduce the density of ground-dwelling birds in protected areas.

    PubMed

    Thompson, Bill

    2015-05-01

    Recreational disturbance associated with trails has been identified as one of the major factors causing a decline of native biodiversity within protected areas. However, despite the negative impacts that recreation can have on biodiversity, providing public access to nature is critical for the future of the conservation of biodiversity. As such, many protected area managers are looking for tools to help maintain a balance between public access and biodiversity conservation. The objectives of this study were to examine the impacts of recreational trails on forest-dwelling bird communities in eastern North America, identify functional guilds which are particularly sensitive to recreational trails, and derive guidelines for trail design to assist in managing the impacts of recreational trails on forest-dwelling birds. Trails within 24 publicly owned natural areas were mapped, and breeding bird communities were described with the use of point count surveys. The density of forest birds, particularly of those species which nest or forage on the ground, were significantly positively influenced by the amount of trail-free refuge habitat. Although management options to control trail use in non-staffed protected areas are limited, this study suggests that protected area managers could design and maintain a trail network that would minimize impacts on resident wildlife, while providing recreational opportunities for visitors, by designing their trail network to maximize the area of trail-free habitat.

  3. Recreational Trails Reduce the Density of Ground-Dwelling Birds in Protected Areas

    NASA Astrophysics Data System (ADS)

    Thompson, Bill

    2015-05-01

    Recreational disturbance associated with trails has been identified as one of the major factors causing a decline of native biodiversity within protected areas. However, despite the negative impacts that recreation can have on biodiversity, providing public access to nature is critical for the future of the conservation of biodiversity. As such, many protected area managers are looking for tools to help maintain a balance between public access and biodiversity conservation. The objectives of this study were to examine the impacts of recreational trails on forest-dwelling bird communities in eastern North America, identify functional guilds which are particularly sensitive to recreational trails, and derive guidelines for trail design to assist in managing the impacts of recreational trails on forest-dwelling birds. Trails within 24 publicly owned natural areas were mapped, and breeding bird communities were described with the use of point count surveys. The density of forest birds, particularly of those species which nest or forage on the ground, were significantly positively influenced by the amount of trail-free refuge habitat. Although management options to control trail use in non-staffed protected areas are limited, this study suggests that protected area managers could design and maintain a trail network that would minimize impacts on resident wildlife, while providing recreational opportunities for visitors, by designing their trail network to maximize the area of trail-free habitat.

  4. Assessing the influence of sustainable trail design and maintenance on soil loss.

    PubMed

    Marion, Jeffrey L; Wimpey, Jeremy

    2017-03-15

    Natural-surfaced trail systems are an important infrastructure component providing a means for accessing remote protected natural area destinations. The condition and usability of trails is a critical concern of land managers charged with providing recreational access while preserving natural conditions, and to visitors seeking high quality recreational opportunities and experiences. While an adequate number of trail management publications provide prescriptive guidance for designing, constructing, and maintaining natural-surfaced trails, surprisingly little research has been directed at providing a scientific basis for this guidance. Results from a review of the literature and three scientific studies are presented to model and clarify the influence of factors that substantially influence trail soil loss and that can be manipulated by trail professionals to sustain high traffic while minimizing soil loss over time. Key factors include trail grade, slope alignment angle, tread drainage features, and the amount of rock in tread substrates. A new Trail Sustainability Rating is developed and offered as a tool for evaluating or improving the sustainability of existing or new trails. Published by Elsevier Ltd.

  5. Assessing the influence of sustainable trail design and maintenance on soil loss

    USGS Publications Warehouse

    Marion, Jeff; Wimpey, Jeremy

    2017-01-01

    Natural-surfaced trail systems are an important infrastructure component providing a means for accessing remote protected natural area destinations. The condition and usability of trails is a critical concern of land managers charged with providing recreational access while preserving natural conditions, and to visitors seeking high quality recreational opportunities and experiences. While an adequate number of trail management publications provide prescriptive guidance for designing, constructing, and maintaining natural-surfaced trails, surprisingly little research has been directed at providing a scientific basis for this guidance. Results from a review of the literature and three scientific studies are presented to model and clarify the influence of factors that substantially influence trail soil loss and that can be manipulated by trail professionals to sustain high traffic while minimizing soil loss over time. Key factors include trail grade, slope alignment angle, tread drainage features, and the amount of rock in tread substrates. A new Trail Sustainability Rating is developed and offered as a tool for evaluating or improving the sustainability of existing or new trails.

  6. 30 CFR 75.601 - Short circuit protection of trailing cables.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... to disconnect power from trailing cables shall be plainly marked and identified and such devices... 30 Mineral Resources 1 2012-07-01 2012-07-01 false Short circuit protection of trailing cables. 75... MINE SAFETY AND HEALTH MANDATORY SAFETY STANDARDS-UNDERGROUND COAL MINES Trailing Cables § 75.601 Short...

  7. 30 CFR 75.601 - Short circuit protection of trailing cables.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... to disconnect power from trailing cables shall be plainly marked and identified and such devices... 30 Mineral Resources 1 2011-07-01 2011-07-01 false Short circuit protection of trailing cables. 75... MINE SAFETY AND HEALTH MANDATORY SAFETY STANDARDS-UNDERGROUND COAL MINES Trailing Cables § 75.601 Short...

  8. 30 CFR 75.601 - Short circuit protection of trailing cables.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... to disconnect power from trailing cables shall be plainly marked and identified and such devices... 30 Mineral Resources 1 2014-07-01 2014-07-01 false Short circuit protection of trailing cables. 75... MINE SAFETY AND HEALTH MANDATORY SAFETY STANDARDS-UNDERGROUND COAL MINES Trailing Cables § 75.601 Short...

  9. 30 CFR 75.601 - Short circuit protection of trailing cables.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... to disconnect power from trailing cables shall be plainly marked and identified and such devices... 30 Mineral Resources 1 2013-07-01 2013-07-01 false Short circuit protection of trailing cables. 75... MINE SAFETY AND HEALTH MANDATORY SAFETY STANDARDS-UNDERGROUND COAL MINES Trailing Cables § 75.601 Short...

  10. Effective Dynamics of Microorganisms That Interact with Their Own Trail

    NASA Astrophysics Data System (ADS)

    Kranz, W. Till; Gelimson, Anatolij; Zhao, Kun; Wong, Gerard C. L.; Golestanian, Ramin

    2016-07-01

    Like ants, some microorganisms are known to leave trails on surfaces to communicate. We explore how trail-mediated self-interaction could affect the behavior of individual microorganisms when diffusive spreading of the trail is negligible on the time scale of the microorganism using a simple phenomenological model for an actively moving particle and a finite-width trail. The effective dynamics of each microorganism takes on the form of a stochastic integral equation with the trail interaction appearing in the form of short-term memory. For a moderate coupling strength below an emergent critical value, the dynamics exhibits effective diffusion in both orientation and position after a phase of superdiffusive reorientation. We report experimental verification of a seemingly counterintuitive perpendicular alignment mechanism that emerges from the model.

  11. Helicopter rotor trailing edge noise. [noise prediction

    NASA Technical Reports Server (NTRS)

    Schlinker, R. H.; Amier, R. K.

    1981-01-01

    A two dimensional section of a helicopter main rotor blade was tested in an acoustic wind tunnel at close to full-scale Reynolds numbers to obtain boundary layer data and acoustic data for use in developing an acoustic scaling law and testing a first principles trailing edge noise theory. Results were extended to the rotating frame coordinate system to develop a helicopter rotor trailing edge noise prediction. Comparisons of the calculated noise levels with helicopter flyover spectra demonstrate that trailing edge noise contributes significantly to the total helicopter noise spectrum at high frequencies. This noise mechanism is expected to control the minimum rotor noise. In the case of noise radiation from a local blade segment, the acoustic directivity pattern is predicted by the first principles trailing edge noise theory. Acoustic spectra are predicted by a scaling law which includes Mach number, boundary layer thickness and observer position. Spectrum shape and sound pressure level are also predicted by the first principles theory but the analysis does not predict the Strouhal value identifying the spectrum peak.

  12. 49 CFR 230.98 - Driving, trailing, and engine truck axles.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false Driving, trailing, and engine truck axles. 230.98... Locomotives and Tenders Running Gear § 230.98 Driving, trailing, and engine truck axles. (a) Condemning defects. Driving, trailing, and engine truck axles with any of the following defects shall be removed from...

  13. 36 CFR 212.51 - Designation of roads, trails, and areas.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Designation of roads, trails... AGRICULTURE TRAVEL MANAGEMENT Designation of Roads, Trails, and Areas for Motor Vehicle Use § 212.51 Designation of roads, trails, and areas. (a) General. Motor vehicle use on National Forest System roads, on...

  14. 49 CFR 230.98 - Driving, trailing, and engine truck axles.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Locomotives and Tenders Running Gear § 230.98 Driving, trailing, and engine truck axles. (a) Condemning defects. Driving, trailing, and engine truck axles with any of the following defects shall be removed from... 49 Transportation 4 2011-10-01 2011-10-01 false Driving, trailing, and engine truck axles. 230.98...

  15. The association of trail use with weather-related factors on an urban greenway.

    PubMed

    Burchfield, Ryan A; Fitzhugh, Eugene C; Bassett, David R

    2012-02-01

    To study the association between weather-related measures and objectively measured trail use across 3 seasons. Weather has been reported as a barrier to outdoor physical activity (PA), but previous studies have explained only a small amount of the variance in PA using weather-related measures. The dependent variable of this study was trail use measured as mean hourly trail counts by an infrared trail counter located on a greenway. Each trail count represents 1 person breaking the infrared beam of the trail counter. Two sources of weather-related measures were obtained by a site-specific weather station and a public domain weather source. Temperature, relative humidity, and precipitation were significantly correlated with trail counts recorded during daylight hours. More precise hourly weather-related measures explained 42% of the variance in trail counts, regardless of the weather data source with temperature alone explaining 18% of the variance in trail counts. After controlling for all seasonal and weekly factors, every 1°F increase in temperature was associated with an increase of 1.1 trail counts/hr up to 76°F, at which point trail use began to slightly decrease. Weather-related factors have a moderate association with trail use along an urban greenway.

  16. ONC201 demonstrates anti-tumor effects in both triple negative and non-triple negative breast cancers through TRAIL-dependent and TRAIL-independent mechanisms

    PubMed Central

    Ralff, Marie D.; Kline, Christina L.B.; Küçükkase, Ozan C; Wagner, Jessica; Lim, Bora; Dicker, David T.; Prabhu, Varun V.; Oster, Wolfgang; El-Deiry, Wafik S.

    2017-01-01

    Breast cancer is a major cause of cancer-related death. TRAIL has been of interest as a cancer therapeutic, but only a subset of triple negative breast cancers (TNBC) is sensitive to TRAIL. The small molecule ONC201 induces expression of TRAIL and its receptor DR5. ONC201 has entered clinical trials in advanced cancers. Here we show that ONC201 is efficacious against both TNBC and non-TNBC cells (n=13). A subset of TNBC and non-TNBC cells succumb to ONC201-induced cell death. In 2/8 TNBC cell lines, ONC201 treatment induces caspase-8 cleavage and cell death that is blocked by TRAIL-neutralizing antibody RIK2. The pro-apoptotic effect of ONC201 translates to in vivo efficacy in the MDA-MB-468 xenograft model. In most TNBC lines tested (6/8) ONC201 has an anti-proliferative effect but does not induce apoptosis. ONC201 decreases cyclin D1 expression and causes an accumulation of cells in the G1 phase of the cell cycle. pRb expression is associated with sensitivity to the anti-proliferative effects of ONC201, and the compound synergizes with taxanes in less sensitive cells. All non-TNBC cells (n=5) are growth inhibited following ONC201 treatment, and unlike what has been observed with TRAIL, a subset (n=2) show PARP cleavage. In these cells, cell death induced by ONC201 is TRAIL-independent. Our data demonstrate that ONC201 has potent anti-proliferative and pro-apoptotic effects in a broad range of breast cancer subtypes, through TRAIL-dependent and TRAIL-independent mechanisms. These findings develop a pre-clinical rationale for developing ONC201 as a single agent and/or in combination with approved therapies in breast cancer. PMID:28424227

  17. 76 FR 26767 - Captain John Smith Chesapeake National Historic Trail Advisory Council

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-09

    ... Trail Advisory Council AGENCY: National Park Service, Interior. ACTION: Notice of meeting. SUMMARY: As... that the Advisory Committee on the Captain John Smith Chesapeake National Historic Trail will hold a meeting. Designated through an amendment to the National Trails System Act (16 U.S.C. 1241), the trail...

  18. 76 FR 52691 - Captain John Smith Chesapeake National Historic Trail Advisory Council

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-23

    ... Trail Advisory Council AGENCY: National Park Service, Interior. ACTION: Notice of meeting. SUMMARY: As... that the Advisory Committee on the Captain John Smith Chesapeake National Historic Trail will hold a meeting. Designated through an amendment to the National Trails System Act (16 U.S.C. 1241), the trail...

  19. Partially turbulated trailing edge cooling passages for gas turbine nozzles

    DOEpatents

    Thatcher, Jonathan Carl; Burdgick, Steven Sebastian

    2001-01-01

    A plurality of passages are spaced one from the other along the length of a trailing edge of a nozzle vane in a gas turbine. The passages lie in communication with a cavity in the vane for flowing cooling air from the cavity through the passages through the tip of the trailing edge into the hot gas path. Each passage is partially turbulated and includes ribs in an aft portion thereof to provide enhanced cooling effects adjacent the tip of the trailing edge. The major portions of the passages are smooth bore. By this arrangement, reduced temperature gradients across the trailing edge metal are provided. Additionally, the inlets to each of the passages have a restriction whereby a reduced magnitude of compressor bleed discharge air is utilized for trailing edge cooling purposes.

  20. Sesquiterpenes with TRAIL-resistance overcoming activity from Xanthium strumarium.

    PubMed

    Karmakar, Utpal K; Ishikawa, Naoki; Toume, Kazufumi; Arai, Midori A; Sadhu, Samir K; Ahmed, Firoj; Ishibashi, Masami

    2015-08-01

    The ability of TRAIL to selectively induce apoptosis in cancer cells while sparing normal cells makes it an attractive target for the development of new cancer therapy. In search of bioactive natural products for overcoming TRAIL-resistance from natural resources, we previously reported a number of active compounds. In our screening program on natural resources targeting overcoming TRAIL-resistance, activity-guided fractionations of the extract of Xanthium strumarium led to the isolation of five sesquiterpene compounds (1-5). 11α,13-dihydroxanthinin (2) and 11α,13-dihydroxanthuminol (3) were first isolated from natural resources and xanthinosin (1), desacetylxanthanol (4), and lasidiol p-methoxybenzoate (5) were known compounds. All compounds (1-5) showed potent TRAIL-resistance overcoming activity at 8, 20, 20, 16, and 16 μM, respectively, in TRAIL-resistant AGS cells. Compounds 1 and 5 enhanced the levels of apoptosis inducing proteins DR4, DR5, p53, CHOP, Bax, cleaved caspase-3, cleaved caspase-8, and cleaved caspase-9 and also decreased the levels of cell survival protein Bcl-2 in TRAIL-resistant AGS cells in a dose-dependent manner. Compound 1 also enhanced the levels of DR4 and DR5 proteins in a time-dependent manner. Thus, compounds 1 and 5 were found to induce both extrinsic and intrinsic apoptotic cell death. Compound 1 also exhibit TRAIL-resistance overcoming activity in DLD1, DU145, HeLa, and MCF7 cells but did not decrease viability in non-cancer HEK293 cells up to 8 μM. Copyright © 2015 Elsevier Ltd. All rights reserved.

  1. 30 CFR 75.830 - Splicing and repair of trailing cables.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Splicing and repair of trailing cables. 75.830... High-Voltage Longwalls § 75.830 Splicing and repair of trailing cables. (a) Splices and repairs. (1... and repairs to high-voltage trailing cables must be made: (i) Only by a qualified person trained in...

  2. 76 FR 8992 - National Trails System Act and Railroad Rights-of-Way

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-16

    ...] National Trails System Act and Railroad Rights-of-Way AGENCY: Surface Transportation Board, DOT. ACTION... procedures regarding the use of railroad rights-of-way for railbanking and interim trail use under the National Trails System Act (Trails Act). DATES: Comments are due by April 12, 2011; replies are due by May...

  3. A cost-benefit analysis of physical activity using bike/pedestrian trails.

    PubMed

    Wang, Guijing; Macera, Caroline A; Scudder-Soucie, Barbara; Schmid, Tom; Pratt, Michael; Buchner, David

    2005-04-01

    From a public health perspective, a cost-benefit analysis of using bike/pedestrian trails in Lincoln, Nebraska, to reduce health care costs associated with inactivity was conducted. Data was obtained from the city's 1998 Recreational Trails Census Report and the literature. Per capita annual cost of using the trails was 209.28 U.S. dollars (59.28 U.S. dollars construction and maintenance, 150 U.S. dollars of equipment and travel). Per capita annual direct medical benefit of using the trails was 564.41 U.S. dollars. The cost-benefit ratio was 2.94, which means that every 1 U.S. dollar investment in trails for physical activity led to 2.94 U.S. dollars in direct medical benefit. The sensitivity analyses indicated the ratios ranged from 1.65 to 13.40. Therefore, building trails is cost beneficial from a public health perspective. The most sensitive parameter affecting the cost-benefit ratios were equipment and travel costs; however, even for the highest cost, every 1 U.S. dollar investment in trails resulted in a greater return in direct medical benefit.

  4. Aeroelastic Response of the Adaptive Compliant Trailing Edge Transtition Section

    NASA Technical Reports Server (NTRS)

    Herrera, Claudia Y.; Spivey, Natalie D.; Lung, Shun-fat

    2016-01-01

    The Adaptive Compliant Trailing Edge demonstrator was a joint task under the Environmentally Responsible Aviation Project in partnership with the Air Force Research Laboratory and FlexSys, Inc. (Ann Arbor, Michigan), chartered by the National Aeronautics and Space Administration to develop advanced technologies that enable environmentally friendly aircraft, such as continuous mold-line technologies. The Adaptive Compliant Trailing Edge demonstrator encompassed replacing the Fowler flaps on the SubsoniC Aircraft Testbed, a Gulfstream III (Gulfstream Aerospace, Savannah, Georgia) aircraft, with control surfaces developed by FlexSys, Inc., a pair of uniquely-designed, unconventional flaps to be used as lifting surfaces during flight-testing to substantiate their structural effectiveness. The unconventional flaps consisted of a main flap section and two transition sections, inboard and outboard, which demonstrated the continuous mold-line technology. Unique characteristics of the transition sections provided a challenge to the airworthiness assessment for this part of the structure. A series of build-up tests and analyses were conducted to ensure the data required to support the airworthiness assessment were acquired and applied accurately. The transition sections were analyzed both as individual components and as part of the flight-test article assembly. Instrumentation was installed in the transition sections based on the analysis to best capture the in-flight aeroelastic response. Flight-testing was conducted and flight data were acquired to validate the analyses. This paper documents the details of the aeroelastic assessment and in-flight response of the transition sections of the unconventional Adaptive Compliant Trailing Edge flaps.

  5. Make a Nature Trail

    ERIC Educational Resources Information Center

    Johnson, Janice K.

    1973-01-01

    Discusses the planning, construction, use, and maintenance of a nature trail. Ideal for demonstrating interrelationships between plants and animals, conservation practices, wildlife management, plant succession, forestry, geologic features and other scientific phenomena. (JR)

  6. Best management practices for erosion control from bladed skid trails

    Treesearch

    Charles R. Wade; W. Michael Aust; M. Chad Bolding; William A. Lakel III

    2012-01-01

    Sediment from forest operations is primarily associated with roads and skid trails. We evaluated five skid trail closure treatments applied to bladed skid trails in the Virginia Piedmont. Closure treatments were Waterbars, Seed, Mulch, Pine slash, and Hardwood slash. Sediment traps were used to collect monthly sediment samples for one year. The Mulch, Pine slash, and...

  7. The dynamics of foraging trails in the tropical arboreal ant Cephalotes goniodontus.

    PubMed

    Gordon, Deborah M

    2012-01-01

    The foraging behavior of the arboreal turtle ant, Cephalotes goniodontus, was studied in the tropical dry forest of western Mexico. The ants collected mostly plant-derived food, including nectar and fluids collected from the edges of wounds on leaves, as well as caterpillar frass and lichen. Foraging trails are on small pieces of ephemeral vegetation, and persist in exactly the same place for 4-8 days, indicating that food sources may be used until they are depleted. The species is polydomous, occupying many nests which are abandoned cavities or ends of broken branches in dead wood. Foraging trails extend from trees with nests to trees with food sources. Observations of marked individuals show that each trail is travelled by a distinct group of foragers. This makes the entire foraging circuit more resilient if a path becomes impassable, since foraging in one trail can continue while a different group of ants forms a new trail. The colony's trails move around the forest from month to month; from one year to the next, only one colony out of five was found in the same location. There is continual searching in the vicinity of trails: ants recruited to bait within 3 bifurcations of a main foraging trail within 4 hours. When bait was offered on one trail, to which ants recruited, foraging activity increased on a different trail, with no bait, connected to the same nest. This suggests that the allocation of foragers to different trails is regulated by interactions at the nest.

  8. The Dynamics of Foraging Trails in the Tropical Arboreal Ant Cephalotes goniodontus

    PubMed Central

    Gordon, Deborah M.

    2012-01-01

    The foraging behavior of the arboreal turtle ant, Cephalotes goniodontus, was studied in the tropical dry forest of western Mexico. The ants collected mostly plant-derived food, including nectar and fluids collected from the edges of wounds on leaves, as well as caterpillar frass and lichen. Foraging trails are on small pieces of ephemeral vegetation, and persist in exactly the same place for 4–8 days, indicating that food sources may be used until they are depleted. The species is polydomous, occupying many nests which are abandoned cavities or ends of broken branches in dead wood. Foraging trails extend from trees with nests to trees with food sources. Observations of marked individuals show that each trail is travelled by a distinct group of foragers. This makes the entire foraging circuit more resilient if a path becomes impassable, since foraging in one trail can continue while a different group of ants forms a new trail. The colony’s trails move around the forest from month to month; from one year to the next, only one colony out of five was found in the same location. There is continual searching in the vicinity of trails: ants recruited to bait within 3 bifurcations of a main foraging trail within 4 hours. When bait was offered on one trail, to which ants recruited, foraging activity increased on a different trail, with no bait, connected to the same nest. This suggests that the allocation of foragers to different trails is regulated by interactions at the nest. PMID:23209749

  9. Characteristics of physical activity levels among trail users in a U.S. national sample.

    PubMed

    Librett, John J; Yore, Michelle M; Schmid, Thomas L

    2006-11-01

    The Task Force on Community Preventive Services strongly recommends environmental interventions that include enhanced access to opportunities for physical activity, such as walking and cycling trails. Although accumulating evidence indicates that trails can be effective in increasing physical activity, little is known about trail users. Cross-sectional analysis of a national sample of 3717 adults from the HealthStyles and ConsumerStyles surveys using logistic regression to determine physical activity patterns and sociodemographic correlates related to trail use, and to identify support regarding trail development policies. Almost 13% (12.7%) of the sample reported using trails at least once a month and 24.3% at least once a week. People who reported using trails at least once a week were twice as likely than people who reported rarely or never using trails to meet physical activity recommendations (odds ratio=2.3, 95% confidence interval=1.9-2.8). Nearly half (43.6%) of the non-trail users supported expanded public spaces for people to exercise, and 36.4% of the non-trail users reported that they would be willing to pay more taxes to build more parks and trails in their community. Community trails facilitate physical activity, and almost half of frequent trail users report that access to trails and other green space is important in choosing a place to live. These results support the need for prospective research on whether newly built trails promote physical activity in previously inactive people.

  10. TNF-related apoptosis-inducing ligand (TRAIL): A new path to anti-cancer therapies

    PubMed Central

    Holoch, Peter A.; Griffith, Thomas S.

    2009-01-01

    Since its discovery in 1995, tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), a member of the tumor necrosis factor super family, has been under intense focus because of its remarkable ability to induce apoptosis in malignant human cells while leaving normal cells unscathed. Consequently, activation of the apoptotic signaling pathway from the death-inducing TRAIL receptors provides an attractive, biologically-targeted approach to cancer therapy. A great deal of research has focused on deciphering the TRAIL receptor signaling cascade and intracellular regulation of this pathway, as many human tumor cells possess mechanisms of resistance to TRAIL-induced apoptosis. This review focuses on the currently state of knowledge regarding TRAIL signaling and resistance, the preclinical development of therapies targeted at TRAIL receptors and modulators of the pathway, and the results of clinical trials for cancer treatment that have emerged from this base of knowledge. TRAIL-based approaches to cancer therapy vary from systemic administration of recombinant, soluble TRAIL protein with or without the combination of traditional chemotherapy, radiation or novel anticancer agents to agonistic monoclonal antibodies directed against functional TRAIL receptors to TRAIL gene transfer therapy. A better understanding of TRAIL resistance mechanisms may allow for the development of more effective therapies that exploit this cell-mediated pathway to apoptosis. PMID:19836385

  11. Roaring Fork Motor Nature Trail, Title Sheet Great Smoky ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Roaring Fork Motor Nature Trail, Title Sheet - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  12. Dyslipidemia and Diabetes Increase the OPG/TRAIL Ratio in the Cardiovascular System.

    PubMed

    Toffoli, Barbara; Fabris, Bruno; Bartelloni, Giacomo; Bossi, Fleur; Bernardi, Stella

    2016-01-01

    Background . Dyslipidemia and diabetes are two of the most well established risk factors for the development of cardiovascular disease (CVD). Both of them usually activate a complex range of pathogenic pathways leading to organ damage. Here we hypothesized that dyslipidemia and diabetes could affect osteoprotegerin (OPG) and TNF-related apoptosis-inducing ligand (TRAIL) expression in the vessels and the heart. Materials and Methods . Gene and protein expression of OPG, TRAIL, and OPG/TRAIL ratio were quantified in the aorta and the hearts of control mice, dyslipidemic mice, and diabetic mice. Results . Diabetes significantly increased OPG and the OPG/TRAIL ratio expression in the aorta, while dyslipidemia was the major determinant of the changes observed in the heart, where it significantly increased OPG and reduced TRAIL expression, thus increasing cardiac OPG/TRAIL ratio. Conclusions . This work shows that both dyslipidemia and diabetes affect OPG/TRAIL ratio in the cardiovascular system. This could contribute to the changes in circulating OPG/TRAIL which are observed in patients with diabetes and CVD. Most importantly, these changes could mediate/contribute to atherosclerosis development and cardiac remodeling.

  13. Via GeoAlpina - an international project of IYPE

    NASA Astrophysics Data System (ADS)

    Piller, Werner E.

    2010-05-01

    Mountainous areas show earth science features in a very conspicuous, frequently even in a spectacular way. Because of the general perception of the beauty of mountains many mountainous regions are well developed in terms of trails and touristic infrastructure. Therefore, mountains are a key area to bring earth sciences closer to people. In many mountain chains all over the world (e.g., Alps, Pyrenees, Andes, Rocky Mountains) far-ranging walking and hiking trails are developed to serve a broad audience. To provide this audience with basic information in geological phenomena along such trails the project "Via Geo..." was born as an international activity within the International Year of Planet Earth. The Alps have been selected to act as a pilot project with six Alpine nations participating: Austria, France, Germany, Italy, Slovenia, and Switzerland. Many well maintained trails exist bridging the Alps from the Adriatic Sea in Trieste (Italy) to Monte Carlo (Monaco) in the Western Mediterranean. Some of these trails are included in the project "Via Alpina". The idea of Via GeoAlpina is to point at geological attractions along the trails of Via Alpina or in their vicinity and offer simple but striking information. Various earth science fields will be covered, such as geology, geophysics, paleontology, mineralogy, hydrogeology, pedology, climatology, and geomorphology. The trail descriptions can be accessed and downloaded from the Via GeoAlpina website. In 2009 every country inaugurated Via GeoAlpina on a national base with a particular opening event in the presence of local authorities and local and national media. These events were accompanied with the distribution of flyers, by offering guided tours and by installation of panels in the field. These activities, however, should be followed by even more actions in the following years. These follow-ups should attract local and regional authorities at state, county or village level and will ideally be financed by private

  14. Health Impact Assessment, Physical Activity and Federal Lands Trail Policy

    PubMed Central

    Davis, Sally M.; Cruz, Theresa H.; Kozoll, Richard L.

    2016-01-01

    Objectives The objectives of this paper are to describe the application of Health Impact Assessment (HIA) to inform trail decisions affecting a rural, under-resourced community and propose the routine integration of HIAs to enhance NEPA environmental assessments and environmental impact statements for trail decisions on federal lands. Methods Screening, scoping, assessment, recommendations, reporting, monitoring and evaluation are being used to examine the health impact of trail location and design. Results HIA recommendations are being integrated into the public lands National Environmental Protection Act process for planning access to a new segment of the Continental Divide National Scenic Trail. Potential users from a nearby rural New Mexico community and a region of almost one million may benefit from this HIA-informed planning. Conclusions HIA can be integrated into the policy and decision-making process for trails on public lands. PMID:27213163

  15. Assessing children's cognitive flexibility with the Shape Trail Test.

    PubMed

    Chan, Amy Y C; Morgan, Sarah-Jane

    2018-01-01

    In this paper we report an initial validation of the Shape Trail Test-Child Version (STT-CV) with a non-clinical sample of children aged 6 to 9 years. The STT-CV has been developed as an age-appropriate and culturally fair direct downward extension of the Trail Making Test (TMT) for the assessment of cognitive flexibility. Children completed the STT-CV and four established measures of executive functions that assessed working memory, inhibitory control and task switching. Results showed the expected age-based differences in completion times for both parts of the STT-CV (Trail A and Trail B). Children's performance on the STT-CV correlated significantly with all four measures of executive functions. After controlling for the effects of chronological age, completion times for Trail B remained correlated with most other measures of executive functions. These findings provide emerging evidence for the utility of the STT-CV, and highlight the need for designing and using appropriate variants of the TMT in the behavioural assessment of cognitive flexibility in developmentally and culturally diverse populations.

  16. 1. Roaring Fork Motor Nature Trail, entrance sign. Great ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. Roaring Fork Motor Nature Trail, entrance sign. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  17. 9. Roaring Fork Motor Nature Trail, Reagan House. Great ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. Roaring Fork Motor Nature Trail, Reagan House. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  18. 30 CFR 75.607 - Breaking trailing cable and power cable connections.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 30 Mineral Resources 1 2013-07-01 2013-07-01 false Breaking trailing cable and power cable....607 Breaking trailing cable and power cable connections. [Statutory Provisions] Trailing cable and power cable connections to junction boxes shall not be made or broken under load. ...

  19. 30 CFR 75.607 - Breaking trailing cable and power cable connections.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 30 Mineral Resources 1 2014-07-01 2014-07-01 false Breaking trailing cable and power cable....607 Breaking trailing cable and power cable connections. [Statutory Provisions] Trailing cable and power cable connections to junction boxes shall not be made or broken under load. ...

  20. 30 CFR 75.607 - Breaking trailing cable and power cable connections.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 30 Mineral Resources 1 2012-07-01 2012-07-01 false Breaking trailing cable and power cable....607 Breaking trailing cable and power cable connections. [Statutory Provisions] Trailing cable and power cable connections to junction boxes shall not be made or broken under load. ...

  1. 30 CFR 75.607 - Breaking trailing cable and power cable connections.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 30 Mineral Resources 1 2011-07-01 2011-07-01 false Breaking trailing cable and power cable....607 Breaking trailing cable and power cable connections. [Statutory Provisions] Trailing cable and power cable connections to junction boxes shall not be made or broken under load. ...

  2. Adoptively transferred TRAIL+ T cells suppress GVHD and augment antitumor activity

    PubMed Central

    Ghosh, Arnab; Dogan, Yildirim; Moroz, Maxim; Holland, Amanda M.; Yim, Nury L.; Rao, Uttam K.; Young, Lauren F.; Tannenbaum, Daniel; Masih, Durva; Velardi, Enrico; Tsai, Jennifer J.; Jenq, Robert R.; Penack, Olaf; Hanash, Alan M.; Smith, Odette M.; Piersanti, Kelly; Lezcano, Cecilia; Murphy, George F.; Liu, Chen; Palomba, M. Lia; Sauer, Martin G.; Sadelain, Michel; Ponomarev, Vladimir; van den Brink, Marcel R.M.

    2013-01-01

    Current strategies to suppress graft-versus-host disease (GVHD) also compromise graft-versus-tumor (GVT) responses. Furthermore, most experimental strategies to separate GVHD and GVT responses merely spare GVT function without actually enhancing it. We have previously shown that endogenously expressed TNF-related apoptosis-inducing ligand (TRAIL) is required for optimal GVT activity against certain malignancies in recipients of allogeneic hematopoietic stem cell transplantation (allo-HSCT). In order to model a donor-derived cellular therapy, we genetically engineered T cells to overexpress TRAIL and adoptively transferred donor-type unsorted TRAIL+ T cells into mouse models of allo-HSCT. We found that murine TRAIL+ T cells induced apoptosis of alloreactive T cells, thereby reducing GVHD in a DR5-dependent manner. Furthermore, murine TRAIL+ T cells mediated enhanced in vitro and in vivo antilymphoma GVT response. Moreover, human TRAIL+ T cells mediated enhanced in vitro cytotoxicity against both human leukemia cell lines and against freshly isolated chronic lymphocytic leukemia (CLL) cells. Finally, as a model of off-the-shelf, donor-unrestricted antitumor cellular therapy, in vitro–generated TRAIL+ precursor T cells from third-party donors also mediated enhanced GVT response in the absence of GVHD. These data indicate that TRAIL-overexpressing donor T cells could potentially enhance the curative potential of allo-HSCT by increasing GVT response and suppressing GVHD. PMID:23676461

  3. 8. Roaring Fork Motor Nature Trail, handbuilt rock pile. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    8. Roaring Fork Motor Nature Trail, hand-built rock pile. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  4. Interferon-induced TRAIL-independent cell death in DNase II-/- embryos.

    PubMed

    Kitahara, Yusuke; Kawane, Kohki; Nagata, Shigekazu

    2010-09-01

    The chromosomal DNA of apoptotic cells and the nuclear DNA expelled from erythroid precursors is cleaved by DNase II in lysosomes after the cells or nuclei are engulfed by macrophages. DNase II(-/-) embryos suffer from lethal anemia due to IFN-beta produced in the macrophages carrying undigested DNA. Here, we show that Type I IFN induced a caspase-dependent cell death in human epithelial cells that were transformed to express a high level of IFN type I receptor. During this death process, a set of genes was strongly activated, one of which encoded TRAIL, a death ligand. A high level of TRAIL mRNA was also found in the fetal liver of the lethally anemic DNase II(-/-) embryos, and a lack of IFN type I receptor in the DNase II(-/-) IFN-IR(-/-) embryos blocked the expression of TRAIL mRNA. However, a null mutation in TRAIL did not rescue the lethal anemia of the DNase II(-/-) embryos, indicating that TRAIL is dispensable for inducing the apoptosis of erythroid cells in DNase II(-/-) embryos, and therefore, that there is a TRAIL-independent mechanism for the IFN-induced apoptosis.

  5. Gingerol sensitizes TRAIL-induced apoptotic cell death of glioblastoma cells

    PubMed Central

    Lee, Dae-Hee; Kim, Dong-Wook; Jung, Chang-Hwa; Lee, Yong J.; Park, Daeho

    2014-01-01

    Glioblastoma multiforme (GBM) is the most lethal and aggressive astrocytoma of primary brain tumors in adults. Although there are many clinical trials to induce the cell death of glioblastoma cells, most glioblastoma cells have been reported to be resistant to TRAIL-induced apoptosis. Here, we showed that gingerol as a major component of ginger can induce TRAIL-mediated apoptosis of glioblastoma. Gingerol increased death receptor (DR) 5 levels in a p53-dependent manner. Furthermore, gingerol decreased the expression level of anti-apoptotic proteins (survivin, c-FLIP, Bcl-2, and XIAP) and increased pro-apoptotic protein, Bax and truncate Bid, by generating reactive oxygen species (ROS).We also found that the sensitizing effects of gingerol in TRAIL-induced cell death were blocked by scavenging ROS or overexpressing anti-apoptotic protein (Bcl-2). Therefore, we showed the functions of gingerol as a sensitizing agent to induce cell death of TRAIL-resistant glioblastoma cells. This study gives rise to the possibility of applying gingerol as an anti-tumor agent that can be used for the purpose of combination treatment with TRAIL in TRAIL-resistant glioblastoma tumor therapy. PMID:25034532

  6. Survey of techniques for reduction of wind turbine blade trailing edge noise.

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Barone, Matthew Franklin

    2011-08-01

    Aerodynamic noise from wind turbine rotors leads to constraints in both rotor design and turbine siting. The primary source of aerodynamic noise on wind turbine rotors is the interaction of turbulent boundary layers on the blades with the blade trailing edges. This report surveys concepts that have been proposed for trailing edge noise reduction, with emphasis on concepts that have been tested at either sub-scale or full-scale. These concepts include trailing edge serrations, low-noise airfoil designs, trailing edge brushes, and porous trailing edges. The demonstrated noise reductions of these concepts are cited, along with their impacts on aerodynamic performance. Anmore » assessment is made of future research opportunities in trailing edge noise reduction for wind turbine rotors.« less

  7. Trailing edge flow conditions as a factor in airfoil design

    NASA Technical Reports Server (NTRS)

    Ormsbee, A. I.; Maughmer, M. D.

    1984-01-01

    Some new developments relevant to the design of single-element airfoils using potential flow methods are presented. In particular, the role played by the non-dimensional trailing edge velocity in design is considered and the relationship between the specified value and the resulting airfoil geometry is explored. In addition, the ramifications of the unbounded trailing edge pressure gradients generally present in the potential flow solution of the flow over an airfoil are examined, and the conditions necessary to obtain a class of airfoils having finite trailing edge pressure gradients developed. The incorporation of these conditions into the inverse method of Eppler is presented and the modified scheme employed to generate a number of airfoils for consideration. The detailed viscous analysis of airfoils having finite trailing edge pressure gradients demonstrates a reduction in the strong inviscid-viscid interactions generally present near the trailing edge of an airfoil.

  8. 30 CFR 77.804 - High-voltage trailing cables; minimum design requirements.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false High-voltage trailing cables; minimum design... OF UNDERGROUND COAL MINES Surface High-Voltage Distribution § 77.804 High-voltage trailing cables; minimum design requirements. (a) High-voltage trailing cables used in resistance grounded systems shall be...

  9. 30 CFR 77.804 - High-voltage trailing cables; minimum design requirements.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 30 Mineral Resources 1 2013-07-01 2013-07-01 false High-voltage trailing cables; minimum design... OF UNDERGROUND COAL MINES Surface High-Voltage Distribution § 77.804 High-voltage trailing cables; minimum design requirements. (a) High-voltage trailing cables used in resistance grounded systems shall be...

  10. 30 CFR 77.804 - High-voltage trailing cables; minimum design requirements.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 30 Mineral Resources 1 2014-07-01 2014-07-01 false High-voltage trailing cables; minimum design... OF UNDERGROUND COAL MINES Surface High-Voltage Distribution § 77.804 High-voltage trailing cables; minimum design requirements. (a) High-voltage trailing cables used in resistance grounded systems shall be...

  11. 30 CFR 77.804 - High-voltage trailing cables; minimum design requirements.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 30 Mineral Resources 1 2012-07-01 2012-07-01 false High-voltage trailing cables; minimum design... OF UNDERGROUND COAL MINES Surface High-Voltage Distribution § 77.804 High-voltage trailing cables; minimum design requirements. (a) High-voltage trailing cables used in resistance grounded systems shall be...

  12. Effectiveness and costs of overland skid trail BMPs

    Treesearch

    Clay Sawyers; W. Michael Aust; M. Chad Bolding; William A. Lakel III

    2012-01-01

    Forestry Best Management Practices (BMPs) are designed to protect water quality; however, little data exists comparing the efficacy and costs of different BMP options for skid trail closure. Study objectives were to evaluate erosion control effectiveness and implementation costs of five overland skid trail closure techniques. Closure techniques were: waterbar only (...

  13. Irigenin sensitizes TRAIL-induced apoptosis via enhancing pro-apoptotic molecules in gastric cancer cells.

    PubMed

    Xu, Ying; Gao, Cheng-Cheng; Pan, Zhen-Guo; Zhou, Chuan-Wen

    2018-02-12

    Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) holds promising value for cancer therapy due to its capacity to induce apoptosis in cancer cells. Nevertheless, TRAIL therapy is greatly hampered by its resistance. Irigenin (Iri), isoflavonoids, can be isolated from the rhizome of Belamcanda chinensis, and has been shown anti-cancer properties. In this study, we explored if Iri could enhance TRAIL-regulated apoptosis in TRAIL resistant gastric cancer cells. Iri significantly potentiated TRAIL-triggered cytotoxicity. Iri alone and TRAIL alone showed no effective role in apoptosis induction, whereas combined treatment with Iri and TRAIL markedly induced apoptosis in cancer cells, as evidenced by the up-regulation of cleaved Caspase-8/-9/-3 and PARP. Additionally, the sensitization to TRAIL was along with the enhancement of pro-apoptotic proteins, including FAS-associated protein with death domain (FADD), death receptor 5 (DR5) and Bax. And suppressing FADD, DR5 and Bax by si RNA significantly reduced the apoptosis and enhanced the cell viability induced by the co-application of Iri and TRAIL. Moreover, the sensitization to TRAIL was accompanied by the decrease of Cellular-FLICE inhibitory protein (c-FLIP), Bcl-2 and Survivin. Additionally, Iri could sensitize TRAIL to produce reactive oxygen species (ROS). Pre-treatment of N-acetyl-cysteine (NAC), ROS scavenger, attenuated Iri plus TRAIL-induced apoptosis and improved cell viability. Finally, combination of Iri and TRAIL inhibited tumor growth in the xenograft model. Collectively, our present study gave new insights into the effects of Iri on potentiating TRAIL-sensitivity, and suggested that Iri could be a potential candidate for sensitizer of TRAIL-resistant cancer cell treatment. Copyright © 2018. Published by Elsevier Inc.

  14. Dichotomous scoring of Trails B in patients referred for a dementia evaluation.

    PubMed

    Schmitt, Andrew L; Livingston, Ronald B; Smernoff, Eric N; Waits, Bethany L; Harris, James B; Davis, Kent M

    2010-04-01

    The Trail Making Test is a popular neuropsychological test and its interpretation has traditionally used time-based scores. This study examined an alternative approach to scoring that is simply based on the examinees' ability to complete the test. If an examinee is able to complete Trails B successfully, they are coded as "completers"; if not, they are coded as "noncompleters." To assess this approach to scoring Trails B, the performance of 97 diagnostically heterogeneous individuals referred for a dementia evaluation was examined. In this sample, 55 individuals successfully completed Trails B and 42 individuals were unable to complete it. Point-biserial correlations indicated a moderate-to-strong association (r(pb)=.73) between the Trails B completion variable and the Total Scale score of the Repeatable Battery for the Assessment of Neurological Status (RBANS), which was larger than the correlation between the Trails B time-based score and the RBANS Total Scale score (r(pb)=.60). As a screen for dementia status, Trails B completion showed a sensitivity of 69% and a specificity of 100% in this sample. These results suggest that dichotomous scoring of Trails B might provide a brief and clinically useful measure of dementia status.

  15. Ambient air conditions and variation in urban trail use.

    PubMed

    Holmes, Ann M; Lindsey, Greg; Qiu, Chenchen

    2009-11-01

    This study examines the effect of air quality and administrative policies on use of urban trails in Indianapolis, IN. Attention is focused on two policy variables: (1) issuance of air pollution advisories and (2) the adoption of Daylight Savings Time. Results suggest that while trail use varies with air quality, current public advisories regarding air pollution may be of limited effectiveness in reducing trail users' exposures to hazardous pollutants. In contrast, the adoption of Daylight Savings Time was associated with a statistically significant increase in traffic levels.

  16. Trail Marking by Caterpillars of the Silverspot Butterfly Dione Juno Huascuma

    PubMed Central

    Pescador-Rubio, Alfonso; Stanford-Camargo, Sergio G.; Páez-Gerardo, Luis E; Ramírez-Reyes, Alberto J.; Ibarra-Jiménez, René A.; Fitzgerald, Terrence D.

    2011-01-01

    A pheromone is implicated in the trail marking behavior of caterpillars of the nymphalid silverspot butterfly, Dione juno huascuma (Reakirt) (Lepidoptera: Heliconiinae) that feed gregariously on Passiflora (Malpighiales: Passifloraceae) vines in Mexico. Although they mark pathways leading from one feeding site to another with silk, this study shows that the silk was neither adequate nor necessary to elicit trail following behavior. Caterpillars marked trails with a long-lived pheromone that was deposited when they brushed the ventral surfaces of the tips of their abdomens along branch pathways. The caterpillars distinguished between pathways deposited by different numbers of siblings and between trails of different ages. Caterpillars also preferentially followed the trails of conspecifics over those of another nymphalid, Nymphalis antiopa L., the mourning cloak butterfly. PMID:21861659

  17. SAHA-induced TRAIL-sensitisation of Multiple Myeloma cells is enhanced in 3D cell culture.

    PubMed

    Arhoma, A; Chantry, A D; Haywood-Small, S L; Cross, N A

    2017-11-15

    Multiple Myeloma (MM) is currently incurable despite many novel therapies. Tumour Necrosis Factor-Related Apoptosis-Inducing Ligand (TRAIL) is a potential anti-tumour agent although effects as a single agent are limited. In this study, we investigated whether the Histone Deacetylase (HDAC) inhibitor SAHA can enhance TRAIL-induced apoptosis and target TRAIL resistance in both suspension culture, and 3D cell culture as a model of disseminated MM lesions that form in bone. The effects of SAHA and/or TRAIL in 6 Multiple Myeloma cell lines were assessed in both suspension cultures and in an Alginate-based 3D cell culture model. The effect of SAHA and/or TRAIL was assessed on apoptosis by assessment of nuclear morphology using Hoechst 33342/Propidium Iodide staining. Viable cell number was assessed by CellTiter-Glo luminescence assay, Caspase-8 and -9 activities were measured by Caspase-Glo™ assay kit. TRAIL-resistant cells were generated by culture of RPMI 8226 and NCI-H929 by acute exposure to TRAIL followed by selection of TRAIL-resistant cells. TRAIL significantly induced apoptosis in a dose-dependent manner in OPM-2, RPMI 8226, NCI-H929, U266, JJN-3 MM cell lines and ADC-1 plasma cell leukaemia cells. SAHA amplified TRAIL responses in all lines except OPM-2, and enhanced TRAIL responses were both via Caspase-8 and -9. SAHA treatment induced growth inhibition that further increased in the combination treatment with TRAIL in MM cells. The co-treatment of TRAIL and SAHA reduced viable cell numbers all cell lines. TRAIL responses were further potentiated by SAHA in 3D cell culture in NCI-H929, RPMI 8226 and U266 at lower TRAIL + SAHA doses than in suspension culture. However TRAIL responses in cells that had been selected for TRAIL resistance were not further enhanced by SAHA treatment. SAHA is a potent sensitizer of TRAIL responses in both TRAIL sensitive and resistant cell lines, in both suspension and 3D culture, however SAHA did not sensitise TRAIL-sensitive cell

  18. 30 CFR 75.827 - Guarding of trailing cables.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Guarding of trailing cables. 75.827 Section 75.827 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR COAL MINE SAFETY AND...-Voltage Longwalls § 75.827 Guarding of trailing cables. (a) Guarding. (1) The high-voltage cable must be...

  19. Indicators and protocols for monitoring impacts of formal and informal trails in protected areas

    USGS Publications Warehouse

    Marion, Jeffrey L.; Leung, Yu-Fai

    2011-01-01

    Trails are a common recreation infrastructure in protected areas and their conditions affect the quality of natural resources and visitor experiences. Various trail impact indicators and assessment protocols have been developed in support of monitoring programs, which are often used for management decision-making or as part of visitor capacity management frameworks. This paper reviews common indicators and assessment protocols for three types of trails, surfaced formal trails, unsurfaced formal trails, and informal (visitor-created) trails. Monitoring methods and selected data from three U.S. National Park Service units are presented to illustrate some common trail impact indicators and assessment options.

  20. Ecological criteria, participant preferences and location models: A GIS approach toward ATV trail planning

    Treesearch

    Stephanie A. Snyder; Jay H. Whitmore; Ingrid E. Schneider; Dennis R. Becker

    2008-01-01

    This paper presents a geographic information system (GIS)-based method for recreational trail location for all-terrain vehicles (ATVs) which considers environmental factors, as well as rider preferences for trail attributes. The method utilizes the Least-Cost Path algorithm within a GIS framework to optimize trail location. The trail location algorithm considered trail...

  1. Equivalence of the Color Trails Test and Trail Making Test in nonnative English-speakers.

    PubMed

    Dugbartey, A T; Townes, B D; Mahurin, R K

    2000-07-01

    The Color Trails Test (CTT) has been described as a culture-fair test of visual attention, graphomotor sequencing, and effortful executive processing abilities relative to the Trail Making Test (TMT). In this study, the equivalence of the TMT and the CTT among a group of 64 bilingual Turkish university students was examined. No difference in performance on the CTT-1 and TMT Part A was found, suggesting functionally equivalent performance across both tasks. In contrast, the statistically significant differences in performance on CTT-2 and TMT Part B, as well as the interference indices for both tests, were interpreted as providing evidence for task nonequivalence of the CTT-2 and TMT Part B. Results have implications for both psychometric test development and clinical cultural neuropsychology.

  2. The interplay between scent trails and group-mass recruitment systems in ants.

    PubMed

    Planqué, Robert; van den Berg, Jan Bouwe; Franks, Nigel R

    2013-10-01

    Large ant colonies invariably use effective scent trails to guide copious ant numbers to food sources. The success of mass recruitment hinges on the involvement of many colony members to lay powerful trails. However, many ant colonies start off as single queens. How do these same colonies forage efficiently when small, thereby overcoming the hurdles to grow large? In this paper, we study the case of combined group and mass recruitment displayed by some ant species. Using mathematical models, we explore to what extent early group recruitment may aid deployment of scent trails, making such trails available at much smaller colony sizes. We show that a competition between group and mass recruitment may cause oscillatory behaviour mediated by scent trails. This results in a further reduction of colony size to establish trails successfully.

  3. Visitor impacts on trails in the Sagarmatha (Mt. Everest) National Park, Nepal.

    PubMed

    Nepal, Sanjay K; Nepal, Stella Amor

    2004-08-01

    This study summarizes findings of a visitor impact study conducted in the Sagarmatha (Mt. Everest) National Park, Nepal. The effect of visitor use, and the influence of environmental characteristics on trail conditions are investigated. Seven trails divided into 208 trail segments, and with a total length of 90 kilometers were included in the assessment. A four-class rating system has been used for the assessment of trail conditions. Arc/Info and Arc/View geographic information system (GIS) are used to analyze spatial patterns of impacts. The study indicates a strong correlation between visitor use and trail degradation. However, locational and environmental factors are equally important variables. The study concludes that more systematic, and experimental studies are needed that can make a clear distinction between human-induced trail damage and the effects of natural factors.

  4. Structural design of morphing trailing edge actuated by SMA

    NASA Astrophysics Data System (ADS)

    Wang, Qi; Xu, Zhiwei; Zhu, Qian

    2013-09-01

    In this paper, the morphing trailing edge is designed to achieve the up and down deflection under the aerodynamic load. After a detailed and accurate computational analysis to determine the SMA specifications and layout programs, a solid model is created in CATIA and the structures of the morphing wing trailing edge are produced by CNC machining. A set of DSP measurement and control system is designed to accomplish the controlling experiment of the morphing wing trailing edge. At last, via the force analysis, the trailing edge is fabricated with four sections of aluminum alloy, and the arrangement scheme of SMA wires is determined. Experiment of precise control integral has been performed to survey the control effect. The experiment consists of deflection angle tests of the third joint and the integral structure. Primarily, the ultimate deflection angle is tested in these two experiments. Therefore, the controlling experiment of different angles could be performed within this range. The results show that the deflection error is less than 4%and response time is less than 6.7 s, the precise controlling of the morphing trailing edge is preliminary realized.

  5. Leading and Trailing Anvil Clouds of West African Squall Lines

    NASA Technical Reports Server (NTRS)

    Centrone, Jasmine; Houze, Robert A.

    2011-01-01

    The anvil clouds of tropical squall-line systems over West Africa have been examined using cloud radar data and divided into those that appear ahead of the leading convective line and those on the trailing side of the system. The leading anvils are generally higher in altitude than the trailing anvil, likely because the hydrometeors in the leading anvil are directly connected to the convective updraft, while the trailing anvil generally extends out of the lower-topped stratiform precipitation region. When the anvils are subdivided into thick, medium, and thin portions, the thick leading anvil is seen to have systematically higher reflectivity than the thick trailing anvil, suggesting that the leading anvil contains numerous larger ice particles owing to its direct connection to the convective region. As the leading anvil ages and thins, it retains its top. The leading anvil appears to add hydrometeors at the highest altitudes, while the trailing anvil is able to moisten a deep layer of the atmosphere.

  6. [Spanish normative studies in a young adult population (NEURONORMA young adults Project): norms for the verbal span, visuospatial span, Letter-Number Sequencing, Trail Making Test and Symbol Digit Modalities Test].

    PubMed

    Tamayo, F; Casals-Coll, M; Sánchez-Benavides, G; Quintana, M; Manero, R M; Rognoni, T; Calvo, L; Palomo, R; Aranciva, F; Peña-Casanova, J

    2012-01-01

    Verbal and visuospatial span, Letter-Number Sequencing, Trail Making Test, and Symbol Digit Modalities Test are frequently used in clinical practice to assess attention, executive functions and memory. In the present study, as part of the Spanish normative studies of NEURONORMA young adults Project, normative data adjusted by age and education are provided for digits, Corsi Block-Tapping Task, Letter-Number Sequencing, Trail Making Test, and Symbol Digit Modalities Test. The sample consisted of 179 participants from 18 to 49 years old, who were cognitively normal. Tables to convert raw scores to scaled scores are provided. Age and education adjusted scores are provided by applying linear regressions. Education affected scores in most of the attention tests; age was found to be related to the visuospatial span and to speed of visuomotor tracking, and there was no relationship as regards sex. The data obtained will be useful in the clinical evaluation of young Spanish adults. Copyright © 2011 Sociedad Española de Neurología. Published by Elsevier Espana. All rights reserved.

  7. Microgravity Induction of TRAIL Expression in Preosteoclast Cells Enhances Osteoclast Differentiation

    NASA Astrophysics Data System (ADS)

    Sambandam, Yuvaraj; Baird, Kelsey L.; Stroebel, Maxwell; Kowal, Emily; Balasubramanian, Sundaravadivel; Reddy, Sakamuri V.

    2016-05-01

    Evidence indicates that astronauts experience significant bone loss in space. We previously showed that simulated microgravity (μXg) using the NASA developed rotary cell culture system (RCCS) enhanced bone resorbing osteoclast (OCL) differentiation. However, the mechanism by which μXg increases OCL formation is unclear. RANK/RANKL signaling pathway is critical for OCL differentiation. Tumor necrosis factor-related apoptosis inducing ligand (TRAIL) has been shown to increase osteoclastogenesis. We hypothesize that TRAIL may play an important role in μXg enhanced OCL differentiation. In this study, we identified by RT profiler PCR array screening that μXg induces high levels of TRAIL expression in murine preosteoclast cells in the absence of RANKL stimulation compared to ground based (Xg) cultures. We further identified that μXg elevated the adaptor protein TRAF-6 and fusion genes OC-STAMP and DC-STAMP expression in preosteoclast cells. Interestingly, neutralizing antibody against TRAIL significantly reduced μXg induced OCL formation. We further identified that over-expression of pTRAIL in RAW 264.7 cells enhanced OCL differentiation. These results indicate that TRAIL signaling plays an important role in the μXg increased OCL differentiation. Therefore, inhibition of TRAIL expression could be an effective countermeasure for μXg induced bone loss.

  8. Home | Trails of Hope: Overland Diaries and Letters, 1846-1869 | Digital

    Science.gov Websites

    Letters, 1846-1869 Home Collections Overland Trails Home Search the Collection All Diaries Trail Maps Photographs & Illustrations Trail Guides Search Browse the Collection Search Browse Images and Text Browse Mormons--Religious Life Religious Life Women Browse Search Browse all Maps Interactive Maps These maps

  9. Validation of Walking Trails for the Urban Training™ of Chronic Obstructive Pulmonary Disease Patients.

    PubMed

    Arbillaga-Etxarri, Ane; Torrent-Pallicer, Jaume; Gimeno-Santos, Elena; Barberan-Garcia, Anael; Delgado, Anna; Balcells, Eva; Rodríguez, Diego A; Vilaró, Jordi; Vall-Casas, Pere; Irurtia, Alfredo; Rodriguez-Roisin, Robert; Garcia-Aymerich, Judith

    2016-01-01

    Accessible interventions to train patients with chronic obstructive pulmonary disease (COPD) are needed. We designed urban trails of different intensities (low, moderate and high) in different types of public spaces (boulevard, beach and park). We aimed to validate the trails' design by assessing the physiological response to unsupervised walking trails of: (1) different intensities in COPD patients, and (2) same intensity from different public spaces in healthy adults. On different days and under standardized conditions, 10 COPD patients walked the three intensity trails designed in a boulevard space, and 10 healthy subjects walked the three intensity trails in three different spaces. We measured physiological response and energy expenditure using a gas analyzer. We compared outcomes across trails intensity and/or spaces using mixed-effects linear regression. In COPD patients, physiological response and energy expenditure increased significantly according to the trails intensity: mean (SD) peak V̇O2 15.9 (3.5), 17.4 (4.7), and 17.7 (4.4) mL/min/kg (p-trend = 0.02), and MET-min 60 (23), 64 (26), 72 (31) (p-trend<0.01) in low, moderate and high intensity trails, respectively. In healthy subjects there were no differences in physiological response to walking trails of the same intensity across different spaces. We validated the trails design for the training of COPD patients by showing that the physiological response to and energy expenditure on unsupervised walking these trails increased according to the predefined trails' intensity and did not change across trails of the same intensity in different public space. Walkable public spaces allow the design of trails that could be used for the training of COPD patients in the community.

  10. Trails of Kilovolt Ions Created by Subsurface Channeling

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Redinger, Alex; Standop, Sebastian; Michely, Thomas

    2010-02-19

    Using scanning tunneling microscopy, we observe the damage trails produced by keV noble-gas ions incident at glancing angles onto Pt(111). Surface vacancies and adatoms aligned along the ion trajectory constitute the ion trails. Atomistic simulations reveal that these straight trails are produced by nuclear (elastic) collisions with surface layer atoms during subsurface channeling of the projectiles. In a small energy window around 5 keV, Xe{sup +} ions create vacancy grooves that mark the ion trajectory with atomic precision. The asymmetry of the adatom production on the two sides of the projectile path is traced back to the asymmetry of themore » ion's subsurface channel.« less

  11. Trail impacts in Sagarmatha (Mt. Everest) National Park, Nepal: a logistic regression analysis.

    PubMed

    Nepal, S K

    2003-09-01

    A trail study was conducted in the Sagarmatha (Mt. Everest) National Park, Nepal, during 1997-1998. Based on that study, this paper examines the spatial variability of trail conditions and analyzes factors that influence trail conditions. Logistic regression (multinomial logit model) is applied to examine the influence of use and environmental factors on trail conditions. The assessment of trail conditions is based on a four-class rating system: (class I, very little damaged; class II, moderately damaged, class III, heavily damaged; and class IV, severely damaged). Wald statistics and a model classification table have been used for data interpretation. Results indicate that altitude, trail gradient, hazard potential, and vegetation type are positively associated with trail condition. Trails are more degraded at higher altitude, on steep gradients, in areas with natural hazard potential, and within shrub/grassland zones. Strong correlations between high levels of trail degradation and higher frequencies of visitors and lodges were found. A detailed analysis of environmental and use factors could provide valuable information to park managers in their decisions about trail design, layout and maintenance, and efficient and effective visitor management strategies. Comparable studies on high alpine environments are needed to predict precisely the effects of topographic and climatic extremes. More refined approaches and experimental methods are necessary to control the effects of environmental factors.

  12. 25 CFR 247.20 - What are the road and trail prohibitions?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 25 Indians 1 2010-04-01 2010-04-01 false What are the road and trail prohibitions? 247.20 Section... RIVER TREATY FISHING ACCESS SITES § 247.20 What are the road and trail prohibitions? (a) You cannot damage or leave in a damaged condition any road, trail, or segment thereof. (b) You cannot block...

  13. Records of Backcountry Use Can Assist Trail Managers

    Treesearch

    H.J. Plumley; H.T. Peet; R.E. Leonard

    1978-01-01

    Records of recreational use of Eastern backcountry areas have not been systematically kept by many trail management groups in the past. Decisions on backcountry site designs and facilities should not be made without information on visitor use and behavior. An analysis of sample data from records of overnight shelter use on the Long Trail, Vermont, indicates how such...

  14. A trail pheromone component of the ant Mayriella overbecki Viehmeyer (Formicidae: Myrmicinae)

    NASA Astrophysics Data System (ADS)

    Kohl, E.; Hölldobler, B.; Bestmann, H.-J.

    The myrmicine ant Mayriella overbecki lays recruitment trails during foraging and nest emigrations. The trail pheromone originates from the poison gland. From ten identified components of the poison gland secretions only methyl 6-methylsalicylate 1 elicited trail following behavior.

  15. Development of a Decision Support System for Monitoring, Reporting, Forecasting Ecological Conditions of the Appalachian Trail

    Treesearch

    Y. Wang; R. Nemani; F. Dieffenbach; K. Stolte; G. Holcomb

    2010-01-01

    This paper introduces a collaborative multi-agency effort to develop an Appalachian Trail (A.T.) MEGA-Transect Decision Support System (DSS) for monitoring, reporting and forecasting ecological conditions of the A.T. and the surrounding lands. The project is to improve decision-making on management of the A.T. by providing a coherent framework for data integration,...

  16. The Influence of Snowmobile Trails on Coyote Movements during Winter in High-Elevation Landscapes

    PubMed Central

    Gese, Eric M.; Dowd, Jennifer L. B.; Aubry, Lise M.

    2013-01-01

    Competition between sympatric carnivores has long been of interest to ecologists. Increased understanding of these interactions can be useful for conservation planning. Increased snowmobile traffic on public lands and in habitats used by Canada lynx (Lynx canadensis) remains controversial due to the concern of coyote (Canis latrans) use of snowmobile trails and potential competition with lynx. Determining the variables influencing coyote use of snowmobile trails has been a priority for managers attempting to conserve lynx and their critical habitat. During 2 winters in northwest Wyoming, we backtracked coyotes for 265 km to determine how varying snow characteristics influenced coyote movements; 278 km of random backtracking was conducted simultaneously for comparison. Despite deep snow (>1 m deep), radio-collared coyotes persisted at high elevations (>2,500 m) year-round. All coyotes used snowmobile trails for some portion of their travel. Coyotes used snowmobile trails for 35% of their travel distance (random: 13%) for a mean distance of 149 m (random: 59 m). Coyote use of snowmobile trails increased as snow depth and penetrability off trails increased. Essentially, snow characteristics were most influential on how much time coyotes spent on snowmobile trails. In the early months of winter, snow depth was low, yet the snow column remained dry and the coyotes traveled off trails. As winter progressed and snow depth increased and snow penetrability increased, coyotes spent more travel distance on snowmobile trails. As spring approached, the snow depth remained high but penetrability decreased, hence coyotes traveled less on snowmobile trails because the snow column off trail was more supportive. Additionally, coyotes traveled closer to snowmobile trails than randomly expected and selected shallower snow when traveling off trails. Coyotes also preferred using snowmobile trails to access ungulate kills. Snow compaction from winter recreation influenced coyote

  17. The influence of snowmobile trails on coyote movements during winter in high-elevation landscapes.

    PubMed

    Gese, Eric M; Dowd, Jennifer L B; Aubry, Lise M

    2013-01-01

    Competition between sympatric carnivores has long been of interest to ecologists. Increased understanding of these interactions can be useful for conservation planning. Increased snowmobile traffic on public lands and in habitats used by Canada lynx (Lynx canadensis) remains controversial due to the concern of coyote (Canis latrans) use of snowmobile trails and potential competition with lynx. Determining the variables influencing coyote use of snowmobile trails has been a priority for managers attempting to conserve lynx and their critical habitat. During 2 winters in northwest Wyoming, we backtracked coyotes for 265 km to determine how varying snow characteristics influenced coyote movements; 278 km of random backtracking was conducted simultaneously for comparison. Despite deep snow (>1 m deep), radio-collared coyotes persisted at high elevations (>2,500 m) year-round. All coyotes used snowmobile trails for some portion of their travel. Coyotes used snowmobile trails for 35% of their travel distance (random: 13%) for a mean distance of 149 m (random: 59 m). Coyote use of snowmobile trails increased as snow depth and penetrability off trails increased. Essentially, snow characteristics were most influential on how much time coyotes spent on snowmobile trails. In the early months of winter, snow depth was low, yet the snow column remained dry and the coyotes traveled off trails. As winter progressed and snow depth increased and snow penetrability increased, coyotes spent more travel distance on snowmobile trails. As spring approached, the snow depth remained high but penetrability decreased, hence coyotes traveled less on snowmobile trails because the snow column off trail was more supportive. Additionally, coyotes traveled closer to snowmobile trails than randomly expected and selected shallower snow when traveling off trails. Coyotes also preferred using snowmobile trails to access ungulate kills. Snow compaction from winter recreation influenced coyote

  18. Trail pheromones: an integrative view of their role in social insect colony organization.

    PubMed

    Czaczkes, Tomer J; Grüter, Christoph; Ratnieks, Francis L W

    2015-01-07

    Trail pheromones do more than simply guide social insect workers from point A to point B. Recent research has revealed additional ways in which they help to regulate colony foraging, often via positive and negative feedback processes that influence the exploitation of the different resources that a colony has knowledge of. Trail pheromones are often complementary or synergistic with other information sources, such as individual memory. Pheromone trails can be composed of two or more pheromones with different functions, and information may be embedded in the trail network geometry. These findings indicate remarkable sophistication in how trail pheromones are used to regulate colony-level behavior, and how trail pheromones are used and deployed at the individual level.

  19. Hexyl decanoate, the first trail pheromone compound identified in a stingless bee, Trigona recursa.

    PubMed

    Jarau, Stefan; Schulz, Claudia M; Hrncir, Michael; Francke, Wittko; Zucchi, Ronaldo; Barth, Friedrich G; Ayasse, Manfred

    2006-07-01

    Foragers of many species of stingless bees guide their nestmates to food sources by means of scent trails deposited on solid substrates between the food and the nest. The corresponding trail pheromones are generally believed to be produced in the mandibular glands, although definitive experimental proof has never been provided. We tested the trail following behavior of recruits of Trigona recursa in field experiments with artificial scent trails branching off from natural scent trails of this stingless bee. First-time recruits (newcomers) did not follow these trails when they were laid with pure solvent or mandibular gland extract. However, they did follow trails made with labial gland extract. Chemical analyses of labial gland secretions revealed that hexyl decanoate was the dominant component (72.4 +/- 1.9% of all volatiles). Newcomers were significantly attracted to artificial trails made with synthetic hexyl decanoate, demonstrating its key function in eliciting scent-following behavior. According to our experiments with T. recursa, the trail pheromone is produced in the labial glands and not in the mandibular glands. Hexyl decanoate is the first component of a trail pheromone identified and proved to be behaviorally active in stingless bees.

  20. Female Sex Pheromone in Trails of the Minute Pirate Bug, Orius minutus (L).

    PubMed

    Maeda, Taro; Fujiwara-Tsujii, Nao; Yasui, Hiroe; Matsuyama, Shigeru

    2016-05-01

    Orius minutus (L.) (Heteroptera: Anthocoridae) is a natural enemy of agricultural pests such as thrips, aphids, and various newly hatched insect juveniles. In this study, we conducted 1) behavioral assays for evidence of contact sex pheromone activity in trails of O. minutus, and 2) chemical analysis to identify the essential chemical components of the trails. Males showed arrestment to trails of mature virgin females but not to trails from either conspecific nymphs or immature females. Females also showed arrestment to trails from conspecific males, although the response was weaker than that exhibited by males. The activity of female trails lasted for at least 46 h after deposition. Males showed a response irrespective of mating experience. Following confirmation that a contact sex pheromone was present in the trails of female O. minutus, we used a bioassay-driven approach to isolate the active chemicals. After fractionation on silica gel, the n-hexane fraction was found to be biologically active to males. A major compound in the active fraction was (Z)-9-nonacosene; this compound was found only in trail extracts of mature virgin females. Synthetic (Z)-9-nonacosene arrested O. minutus males, indicating that it is the major active component of the contact sex pheromone in the trails of female O. minutus.

  1. Sediment pathways in a tropical forest: effects of logging roads and skid trails

    NASA Astrophysics Data System (ADS)

    Sidle, Roy C.; Sasaki, Shozo; Otsuki, Mieko; Noguchi, Shoji; Rahim Nik, Abdul

    2004-03-01

    Significant erosion occurred from recently constructed forest logging roads and skid trails in a small headwater catchment in Peninsular Malaysia. Soil loss was estimated by measuring dimensions of all significant rills and gullies along the road, as well as by measuring height of preserved soil pedestals in sidecast and fill material and on skid trails. Estimates of surface erosion from logging roads and skid trails were 272 +/- 20 t ha-1 year-1 and 275 +/- 20 t ha-1 year-1 respectively. However, owing to lack of connectivity of skid trails to the stream, much of the sediment mobilized on skid trails was stored either on adjacent hillslopes or the trails themselves, rather than being transported to the stream system, as was the case for the road. Steeper skid trails (>20% gradient) had slightly higher erosion rates (320 +/- 24 t ha-1 year-1) than trails with gentler gradients (245-264 t ha-1 year-1). Some 60% of the soil loss on logging roads comes from erosion of the running surface. Disturbed cut and fill material along the road supplied the remaining 40% of the soil loss from roads. Roads and skid trails had no designed drainage systems; runoff discharged onto the hillslope at 25 major discharge nodes from the logging road (690 m total length) and at 34 nodes from skid trails (2300 m). Sediment pathways were either fully or moderately connected to headwater channels at 64% of the logging road nodes, but at only 26% of the nodes emanating from skid trails. A detailed sediment budget revealed that 78% of the soil loss from the road system (including log landings) was delivered to the stream in the first 16 months after logging began. Most (90%) of the deposition from skid trails occurred below just three discharge nodes. Runoff from and onto skid trails often exacerbated the sediment connectivity to channels. Clearly, sediment discharge from logging roads was more highly connected to the stream than discharge from skid trails. Once in the channel, much of this

  2. Static Extended Trailing Edge for Lift Enhancement: Experimental and Computational Studies

    NASA Technical Reports Server (NTRS)

    Liu, Tianshu; Montefort; Liou, William W.; Pantula, Srinivasa R.; Shams, Qamar A.

    2007-01-01

    A static extended trailing edge attached to a NACA0012 airfoil section is studied for achieving lift enhancement at a small drag penalty. It is indicated that the thin extended trailing edge can enhance the lift while the zero-lift drag is not significantly increased. Experiments and calculations are conducted to compare the aerodynamic characteristics of the extended trailing edge with those of Gurney flap and conventional flap. The extended trailing edge, as a simple mechanical device added on a wing without altering the basic configuration, has a good potential to improve the cruise flight efficiency.

  3. Trail and arena marking by caterpillars ofArchips cerasivoranus (lepidoptera: Tortricidae).

    PubMed

    Fitzgerald, T D

    1993-07-01

    The activity ofArchips cerasivoranus caterpillars is largely limited to their colonial silk web and trails. Silk pulled directly from the spinnerets of caterpillars and wound onto paper strips to form artificial trails elicited locomotion from the larvae. Trails made from extracts of silk and silk glands also elicited locomotion. These and other observations reported here indicate that the caterpillars are responsive to a water-soluble pheromone that is a component of the silk strand. Marker pheromones appear not to be secreted from other regions of the body, as has been reported for some other trail-following caterpillars.

  4. Estimating soil erosion on hiking trails in the Sierra Mariola Natural Park in southern Spain

    NASA Astrophysics Data System (ADS)

    Magdalena Warter, Maria; Peeters, Mattias; Kuppen, Emiel; Blok, Kas; Dilly, Lina

    2017-04-01

    Natural parks and protected natural areas provide excellent recreational opportunities for outdoor activities through the richness of the natural environment and the abundance of walking trails. Hiking, mountain biking and running have rapidly gained popularity over recent years increasing concerns about the erosion and degradation of hiking trails caused by (over)use. This is also the case in the Sierra Mariola Natural Park in southeast Spain, which is a popular destination for tourists due to its diverse fauna and flora. The increasing number of tourists together with the negative impacts of climate change necessitates a better understanding of the key soil erosion processes impacting hiking trails. There are 4 scenic trail routes in the Natural Park amounting to 21 km plus an additional network of unofficial trails. Apart from the heavy touristic traffic on the trails there are large trail running events with up to 1000 participants becoming increasingly popular, however local park authorities have voiced concerns about the impacts of these activities on the trails. Despite the popularity of walking trails around the world, there is a paucity of research exploring soil erosion from these features. Therefore, the aims of this study are: 1) to ascertain the amount of erosion that occurs on trails in the Sierra Mariola Natural Park, and 2) determine the key factors that influence soil erosion. Some 100 km of trails were evaluated (both official and unmarked trails), with route segments ranging between 2 and 10 km. A trail classification system was developed to group trail segments based on their surface characteristics (bedrock, gravel, mixed sediment, soil or man-made) and specific erosion features (rills, ditch-shaped, tilted). For each class, the average erosion rate was calculated which ranged from 262 t/ha for soil-based trails to 2006 t/ha for heavily eroded, ditch-shaped trails. The spatial distribution of the different erosion rates and trail types were

  5. Modeling and mapping urban bicyclists' preferences for trail environments.

    Treesearch

    Dawn Wiberg-Carlson; Herbert Schroeder

    1992-01-01

    Derives a model for predicting preferences of seventh and eighth graders for settings along an urban bicycle trail. The rises and falls in preference along the length of the trail are profiled and mapped to display the areas of highest and lowest perceived quality.

  6. Is the color trails culture free?

    PubMed

    Fasfous, Ahmed F; Puente, Antonio E; Pérez-Marfil, María Nieves; Cruz-Quintana, Francisco; Peralta-Ramirez, Isabel; Pérez-García, Miguel

    2013-11-01

    Increasingly clinical neuropsychology has been addressing the effects of culture on neuropsychological functioning. However, that focus has been on comparing performance on standardized tests across two or more groups, often Hispanic. In this study, Arabic children were tested in Morocco using a "culture-free test," Children's Color Trails. Children of different ages and living in rural and urban centers were tested. The results suggest that the Color Trails Test scores from Arab children differed from U.S. norms available. Furthermore, the location of testing and the age of the child were of significance. The role of culture-specific tests was considered.

  7. Wake-Induced Aerodynamics on a Trailing Aircraft

    NASA Technical Reports Server (NTRS)

    Mendenhall, Michael R.; Lesieutre, Daniel J.; Kelly, Michael J.

    2016-01-01

    NASA conducted flight tests to measure the exhaust products from alternative fuels using a DC-8 transport aircraft and a Falcon business jet. An independent analysis of the maximum vortex-induced loads on the Falcon in the DC-8 wake was conducted for pre-flight safety analysis and to define safe trail distances for the flight tests. Static and dynamic vortex-induced aerodynamic loads on the Falcon were predicted at a matrix of locations aft of the DC-8 under flight-test conditions, and the maximum loads were compared with design limit loads to assess aircraft safety. Trajectory simulations for the Falcon during close encounters with the DC-8 wake were made to study the vortex-induced loads during traverses of the DC-8 primary trailing vortex. A parametric study of flight traverses through the trailing vortex was conducted to assess Falcon flight behavior and motion characteristics.

  8. Rock Levitation by Water and Ice; an Explanation for Trails in Racetrack Playa, California

    NASA Astrophysics Data System (ADS)

    Kletetschka, G.; Ryan, A.; McKinney, E.; Fercana, G.; Schwebler, K. P.; McIntire, L.; Miller, D.; Fox, V. K.; Marbourg, J. M.; Naquin, C. A.; Krzykowski, M.; Wilde, J. R.; Kopp, E. S.; Romine, G.; Yawn, K.; Schoch, I.; McAdam, M.; Burger, D.; Rilee, K.; Jackson, B. K.; Parsons, A. M.; Cheung, C. Y.; Lunar; Planetary Science Academy

    2010-12-01

    Through a process that is nearly a century-old mystery, rock fragments race over a desiccated layer of sediment in the California desert, forming the infamous rock trails of the Racetrack playa, found in Death Valley, California. Rocks, randomly distributed over the playa, have indented grooves or trails next to them, appearing as if someone had dragged them over the playa surface when wet. Interestingly, no one has ever witnessed the movement of these rocks. Furthermore, the mechanism responsible for these trails behind the rocks has not yet been explained. Rocks have masses ranging from 0.5 kg to 300 kg, and the trails have a chaotic character, with some trails as long as 1/2 km. Each rock has a mound of raised clay on one side and a mud trail on the other; no other unusual marks are visible. A number of trails have no rocks at the end, with only a mound of solid clay where a rock once appeared to be, as if something was pushing the clay forwards to make the trail but disappeared after the trail was made. Measurements of the humidity and temperature of the sediment pointed towards a unique mechanism of how the trails could form on their own and how simple environmental changes could result in the aforementioned trails in the sediment.

  9. Accurate Natural Trail Detection Using a Combination of a Deep Neural Network and Dynamic Programming.

    PubMed

    Adhikari, Shyam Prasad; Yang, Changju; Slot, Krzysztof; Kim, Hyongsuk

    2018-01-10

    This paper presents a vision sensor-based solution to the challenging problem of detecting and following trails in highly unstructured natural environments like forests, rural areas and mountains, using a combination of a deep neural network and dynamic programming. The deep neural network (DNN) concept has recently emerged as a very effective tool for processing vision sensor signals. A patch-based DNN is trained with supervised data to classify fixed-size image patches into "trail" and "non-trail" categories, and reshaped to a fully convolutional architecture to produce trail segmentation map for arbitrary-sized input images. As trail and non-trail patches do not exhibit clearly defined shapes or forms, the patch-based classifier is prone to misclassification, and produces sub-optimal trail segmentation maps. Dynamic programming is introduced to find an optimal trail on the sub-optimal DNN output map. Experimental results showing accurate trail detection for real-world trail datasets captured with a head mounted vision system are presented.

  10. TRAIL Enhances Shikonin Induced Apoptosis through ROS/JNK Signaling in Cholangiocarcinoma Cells.

    PubMed

    Zhou, Guangyao; Yang, Zuqin; Wang, Xiaodong; Tao, Ran; Zhou, Yuanping

    2017-01-01

    Cholangiocarcinoma (CCA), arising from varying locations within the biliary tree, is the second most common primary liver malignancy worldwide. Shikonin, an active compound extracted from the Chinese herb Zicao, holds anti-bacterial, anti-inflammatory, and anti-tumor activities. However, the effect of shikonin on human cholangiocarcinoma and detailed mechanisms of TRAIL enhancement remains to be elucidated. The purpose of the study was to investigate the protective functions of TRAIL enhancement for shikonin induced apoptosis in cholangiocarcinoma cells. We use MTT assay, apoptosis assay, caspase activity assay, flow cytometry assay, real time PCR and Western blot to observe the effects of TRAIL on shikonin induced cholangiocarcinoma cells apoptosis and its mechanism. Shikonin inhibited cell viability and induced apoptosis of CCA cells, effects enhanced by TRAIL treatment via activation of caspase-3, -8, -9. Furhermore, TRAIL enhanced anti-proliferation of shikonin and shikonin induced apoptosis through induction of ROS mediated JNK activation, while AKT activation had an effect on shikonin anti-proliferation activity, but not in the TRAIL enhanced counterparts. Finally, shikonin upregulated DR5 expression, an effect essential for TRAIL-enhanced activities of shikonin in RBE cells. Our results revealed that shikonin could inhibit cells viability and induce apoptosis of CCA cells, effects enhanced by TRAIL treatment via ROS mediated JNK signalling pathways, involving up-regulation of DR5 expression. Our results provide further insight into the mechanism underlying the anti-tumor effects of shikonin by TRAIL enhanced in CCA and a new therapeutic strategy to CCA treatment. © 2017 The Author(s). Published by S. Karger AG, Basel.

  11. Scent trailing by virgin females ofPseudococcm calceolariae.

    PubMed

    Rotundo, G; Tremblay, E

    1981-01-01

    Virgin females of the citrophilous mealybugPseudococcus calceolariae (Mask.) deposit scent marks as trails on the substrate on which they rest or move. These substances elicit attraction and sexual behavior by conspecific males. The same responses were obtained when males were bioassayed on extracts from filter paper disks on which females had rested. The significance of scent trailing in mealybugs is discussed.

  12. Cohort Profile Update: The TRacking Adolescents’ Individual Lives Survey (TRAILS)

    PubMed Central

    Oldehinkel, Albertine J; Rosmalen, Judith GM; Buitelaar, Jan K; Hoek, Hans W; Ormel, Johan; Raven, Dennis; Reijneveld, Sijmen A; Veenstra, René; Verhulst, Frank C; Vollebergh, Wilma AM; Hartman, Catharina A

    2015-01-01

    TRAILS consists of a population cohort (N = 2230) and a clinical cohort (N = 543), both of which were followed from about age 11 years onwards. To date, the population cohort has been assessed five times over a period of 11 years, with retention rates ranging between 80% and 96%. The clinical cohort has been assessed four times over a period of 8 years, with retention rates ranging between 77% and 85%. Since the IJE published a cohort profile on the TRAILS in 2008, the participants have matured from adolescents into young adults. The focus shifted from parents and school to entry into the labour market and family formation, including offspring. Furthermore, psychiatric diagnostic interviews were administered, the database was linked to a Psychiatric Case Registry, and the availability of genome-wide SNP variations opened the door to genome-wide association studies regarding a wide range of (endo)phenotypes. With some delay, TRAILS data are available to researchers outside the TRAILS consortium without costs; access can be obtained by submitting a publication proposal (see www.trails.nl). PMID:25431468

  13. pIL6-TRAIL-engineered umbilical cord mesenchymal/stromal stem cells are highly cytotoxic for myeloma cells both in vitro and in vivo.

    PubMed

    Cafforio, Paola; Viggiano, Luigi; Mannavola, Francesco; Pellè, Eleonora; Caporusso, Concetta; Maiorano, Eugenio; Felici, Claudia; Silvestris, Francesco

    2017-09-29

    Mesenchymal/stromal stem cells (MSCs) are favorably regarded in anti-cancer cytotherapies for their spontaneous chemotaxis toward inflammatory and tumor environments associated with an intrinsic cytotoxicity against tumor cells. Placenta-derived or TRAIL-engineered adipose MSCs have been shown to exert anti-tumor activity in both in-vitro and in-vivo models of multiple myeloma (MM) while TRAIL-transduced umbilical cord (UC)-MSCs appear efficient inducers of apoptosis in a few solid tumors. However, apoptosis is not selective for cancer cells since specific TRAIL receptors are also expressed by a number of normal cells. To overcome this drawback, we propose to transduce UC-MSCs with a bicistronic vector including the TRAIL sequence under the control of IL-6 promoter (pIL6) whose transcriptional activation is promoted by the MM milieu. UC-MSCs were transduced with a bicistronic retroviral vector (pMIGR1) encoding for green fluorescent protein (GFP) and modified to include the pIL6 sequence upstream of the full-length human TRAIL cDNA. TRAIL expression after stimulation with U-266 cell conditioned medium, or IL-1α/IL-1β, was evaluated by flow cytometry, confocal microscopy, real-time PCR, western blot analysis, and ELISA. Apoptosis in MM cells was assayed by Annexin V staining and by caspase-8 activation. The cytotoxic effect of pIL6-TRAIL + -GFP + -UC-MSCs on MM growth was evaluated in SCID mice by bioluminescence and ex vivo by caspase-3 activation and X-ray imaging. Statistical analyses were performed by Student's t test, ANOVA, and logrank test for survival curves. pIL6-TRAIL + -GFP + -UC-MSCs significantly expressed TRAIL after stimulation by either conditioned medium or by IL-1α/IL-1β, and induced apoptosis in U-266 cells. Moreover, when systemically injected in SCID mice intratibially xenografted with U-266, those cells underwent within MM tibia lesions and significantly reduced the tumor burden by specific induction of apoptosis in MM cells as revealed by

  14. 30 CFR 75.907 - Design of trailing cables for medium-voltage circuits.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Design of trailing cables for medium-voltage... Medium-Voltage Alternating Current Circuits § 75.907 Design of trailing cables for medium-voltage circuits. [Statutory Provisions] Trailing cables for medium-voltage circuits shall include grounding...

  15. 30 CFR 75.907 - Design of trailing cables for medium-voltage circuits.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 30 Mineral Resources 1 2011-07-01 2011-07-01 false Design of trailing cables for medium-voltage... Medium-Voltage Alternating Current Circuits § 75.907 Design of trailing cables for medium-voltage circuits. [Statutory Provisions] Trailing cables for medium-voltage circuits shall include grounding...

  16. 30 CFR 75.907 - Design of trailing cables for medium-voltage circuits.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 30 Mineral Resources 1 2013-07-01 2013-07-01 false Design of trailing cables for medium-voltage... Medium-Voltage Alternating Current Circuits § 75.907 Design of trailing cables for medium-voltage circuits. [Statutory Provisions] Trailing cables for medium-voltage circuits shall include grounding...

  17. 30 CFR 75.907 - Design of trailing cables for medium-voltage circuits.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 30 Mineral Resources 1 2012-07-01 2012-07-01 false Design of trailing cables for medium-voltage... Medium-Voltage Alternating Current Circuits § 75.907 Design of trailing cables for medium-voltage circuits. [Statutory Provisions] Trailing cables for medium-voltage circuits shall include grounding...

  18. 30 CFR 75.907 - Design of trailing cables for medium-voltage circuits.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 30 Mineral Resources 1 2014-07-01 2014-07-01 false Design of trailing cables for medium-voltage... Medium-Voltage Alternating Current Circuits § 75.907 Design of trailing cables for medium-voltage circuits. [Statutory Provisions] Trailing cables for medium-voltage circuits shall include grounding...

  19. Observations of Aircraft Dissipation Trails from GOES

    NASA Technical Reports Server (NTRS)

    Duda, David P.; Minnis, Patrick

    2002-01-01

    Two cases of distrails (aircraft dissipation trails) with associated fall streak clouds were analyzed using multispectral geostationary satellite data. One distrail was observed on 23 July 2000 in a single cloud layer over southeastern Virginia and the Chesapeake Bay. Another set of trails developed on 6 January 2000 at the top of multilayer clouds off the coasts of Georgia and South Carolina. The distrails on both days formed in optically thin, midlevel stratified clouds with cloud-top heights between 7.6 and 9.1 km. The distrail features remained intact and easily visible from satellite images for 1-2 h in spite of winds near 50 km at cloud level. The width of the distrails spread as far as 20 km within 90 min or less. Differences between the optical properties of the clouds surrounding the trails and those of the fall streak particles inside the distrails allowed for easy identification of the fall streak clouds in either the 3.9-micrometer brightness temperature imagery, or the 10.7-micrometer - 12.0-micrometer brightness temperature difference. Although the three-channel infrared retrieval was better at retrieving cloud properties in the multilayer cloud case, two independent remote sensing retrievals of both distrail cases showed that the fall streaks had larger particle sizes than the clouds outside of the trails.

  20. Influence of TRAIL gene on biomechanical properties of the human leukemic cell line Jurkat.

    PubMed

    Yao, Weijuan; Chen, Kai; Wang, Xinjuan; Xie, Lide; Wen, Zongyao; Yan, Zongyi; Chien, Shu

    2002-12-01

    We cloned the cDNA fragment of human TNF-related apoptosis inducing ligand (TRAIL) into RevTet-On, a Tet-regulated and high-level gene expression system. Making use of the TRAIL gene expression system in Jurkat as a cell model, we studied the influence of TRAIL gene on the biomechanics properties of Jurkat through measuring changes of cellular biomechanics properties before and after the TRAIL gene expression, which was induced by adding tetracycline derivative doxycycline (Dox). The results indicated that the TRAIL gene expression led to significant changes in cellular biomechanics properties. The osmotic fragility increased and the cell stiffness increased after the expression of TRAIL gene. Thus, the apoptosis-inducing TRAIL gene caused significant changes in the biomechanics properties of Jurkat cells.

  1. 7. SHOWING METHOD OF SLEDDING WIND CABLE DOWN YAKI TRAIL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. SHOWING METHOD OF SLEDDING WIND CABLE DOWN YAKI TRAIL TO THE BRIDGE, WEIGHT OF CABLE AND DRUM APPROXIMATELY 2200 POUNDS - Kaibab Trail Suspension Bridge, Spanning Colorado River, Grand Canyon, Coconino County, AZ

  2. Fiscal Year 2013 Trails Management Program Mitigation Action Plan Annual Report, October 2013

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Pava, Daniel S.

    This Trails Management Program Mitigation Action Plan Annual Report (Trails MAPAR) has been prepared for the Department of Energy (DOE)/National Nuclear Security Administration (NNSA) as part of implementing the 2003 Final Environmental Assessment for the Proposed Los Alamos National Laboratory Trails Management Program (DOE 2003). The Trails Mitigation Action Plan (MAP) is now a part of the Site-Wide Environmental Impact Statement for the Continued Operation of Los Alamos National Laboratory (DOE/EIS 0380) Mitigation Action Plan (2008 SWEIS MAP) (DOE 2008). The MAP provides guidance for the continued implementation of the Trails Management Program at Los Alamos National Laboratory (LANL) andmore » integration of future mitigation actions into the 2008 SWEIS MAP to decrease impacts associated with recreational trails use at LANL. This eighth MAPAR includes a summary of Trails Management Program activities and actions during Fiscal Year (FY) 2013, from October 2012 through September 2013.« less

  3. Stingless bees (Scaptotrigona pectoralis) learn foreign trail pheromones and use them to find food.

    PubMed

    Reichle, Christian; Aguilar, Ingrid; Ayasse, Manfred; Jarau, Stefan

    2011-03-01

    Foragers of several species of stingless bees (Hymenoptera, Apidae and Meliponini) deposit pheromone marks in the vegetation to guide nestmates to new food sources. These pheromones are produced in the labial glands and are nest and species specific. Thus, an important question is how recruited foragers recognize their nestmates' pheromone in the field. We tested whether naïve workers learn a specific trail pheromone composition while being recruited by nestmates inside the hive in the species Scaptotrigona pectoralis. We installed artificial scent trails branching off from trails deposited by recruiting foragers and registered whether newly recruited bees follow these trails. The artificial trails were baited with trail pheromones of workers collected from foreign S. pectoralis colonies. When the same foreign trail pheromone was presented inside the experimental hives while recruitment took place a significant higher number of bees followed the artificial trails than in experiments without intranidal presentation. Our results demonstrate that recruits of S. pectoralis can learn the composition of specific trail pheromone bouquets inside the nest and subsequently follow this pheromone in the field. We, therefore, suggest that trail pheromone recognition in S. pectoralis is based on a flexible learning process rather than being a genetically fixed behaviour.

  4. Laboratory investigations of the trail-following responses of four species of leaf-cutting ants with notes on the specificity of a trail pheromone of Atta texana (Buckley)

    Treesearch

    S. W. Robinson; John C. Moser; M. S. Blum; E. Amante

    1974-01-01

    Interspecific trail following activity of poison sac contents from four species of leaf-cutting ants was investigated. with only one exception, all the species teste followed trails made from each others poison sac contents. however when the ants were given a choice of following one of two separate trails, clear differences were shown in the poison sac contents of the...

  5. Numerical Studies of High-Intensity Injection Painting for Project X

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Drozhdin, A.I.; Vorobiev, L.G.; Johnson, D.E.

    Injection phase space painting enables the mitigation of space charge and stability issues, and will be indispensable for the Project-X at Fermilab [1], delivering high-intensity proton beams to HEP experiments. Numerical simulations of multi-turn phase space painting have been performed for the FNAL Recycler Ring, including a self-consistent space charge model. The goal of our studies was to study the injection painting with inclusion of 3D space charge, using the ORBIT tracking code. In a current scenario the painting lasts for 110 turns, twice faster, than we considered in this paper. The optimal wave-forms for painting kickers, which ensure themore » flatter phase distributions, should be found. So far we used a simplified model for painting kicker strength (implemented as the 'ideal bump' in ORBIT). We will include a more realistic field map for the chicane magnets. Additional stripping simulations will be combined. We developed a block for longitudinal painting, which works with arbitrary notches in incoming micro-bunch buckets. The appropriate choice of the amplitude of the second harmonic of RF field will help to flatten the RF-bucket contours, as was demonstrated in 1D simulations. Non-linear lattice issue will be also addressed.« less

  6. 5. Roaring Fork Motor Nature Trail, vista at stop three. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. Roaring Fork Motor Nature Trail, vista at stop three. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  7. Metformin sensitizes triple-negative breast cancer to proapoptotic TRAIL receptor agonists by suppressing XIAP expression.

    PubMed

    Strekalova, Elena; Malin, Dmitry; Rajanala, Harisha; Cryns, Vincent L

    2017-06-01

    Despite robust antitumor activity in diverse preclinical models, TNF-related apoptosis-inducing ligand (TRAIL) receptor agonists have not demonstrated efficacy in clinical trials, underscoring the need to identify agents that enhance their activity. We postulated that the metabolic stress induced by the diabetes drug metformin would sensitize breast cancer cells to TRAIL receptor agonists. Human triple (estrogen receptor, progesterone receptor, and HER2)-negative breast cancer (TNBC) cell lines were treated with TRAIL receptor agonists (monoclonal antibodies or TRAIL peptide), metformin, or the combination. The effects on cell survival, caspase activation, and expression of TRAIL receptors and the antiapoptotic protein XIAP were determined. In addition, XIAP was silenced by RNAi in TNBC cells and the effects on sensitivity to TRAIL were determined. The antitumor effects of metformin, TRAIL, or the combination were evaluated in an orthotopic model of metastatic TNBC. Metformin sensitized diverse TNBC cells to TRAIL receptor agonists. Metformin selectively enhanced the sensitivity of transformed breast epithelial cells to TRAIL receptor agonist-induced caspase activation and apoptosis with little effect on untransformed breast epithelial cells. These effects of metformin were accompanied by robust reductions in the protein levels of XIAP, a negative regulator of TRAIL-induced apoptosis. Silencing XIAP in TNBC cells mimicked the TRAIL-sensitizing effects of metformin. Metformin also enhanced the antitumor effects of TRAIL in a metastatic murine TNBC model. Our findings indicate that metformin enhances the activity of TRAIL receptor agonists, thereby supporting the rationale for additional translational studies combining these agents.

  8. Expression of TRAIL and Fas in Primary Hyperparathyroidism.

    PubMed

    Segiet, Oliwia Anna; Deska, Mariusz; Mielańczyk, Łukasz; Brzozowa-Zasada, Marlena; Buła, Grzegorz; Gawrychowski, Jacek; Wojnicz, Romuald

    2017-08-01

    Differentiating between parathyroid lesions is still difficult and ambiguous. In cases of primary hyperparathyroidism, appropriate and prompt diagnosis is of great importance for effective treatment and follow-up. A great amount of mechanisms contribute to the pathogenesis of primary hyperparathyroidism, such as disturbance in balance between pro- and anti-apoptotic factors. Therefore, we examined whether immunohistochemical expression of apoptotic factors, TNF-related apoptosis-inducing ligand (TRAIL) and Fas, could have clinical utility as a marker of proliferative lesions of parathyroid gland. Parathyroid specimens of 58 consecutive patients who had undertaken surgery due to primary hyperparathyroidism were incubated with purified mouse monoclonal antihuman antibodies: anti-TRAIL and anti-Fas. Staining was considered positive when at least 5% of the cells showed immunoreactivity. The percentage of cells which were positively stained for TRAIL in parathyroid hyperplasia was 9.65%, in parathyroid adenoma 8.31%, and in normal controls 2.24%. Immunoreactivity for TRAIL was detected in 91.89% of parathyroid hyperplasias, 85.71% of parathyroid adenomas, and none in healthy glands. The percentage of cells with a positive reaction to Fas in parathyroid hyperplasia was 8.92%, in parathyroid adenoma 8.09%, and in normal tissue 1.9%. The expression of Fas was found in 94.59% of parathyroid hyperplasias, 90.48% of parathyroid adenomas, and none in healthy glands. In our study, hyperplasias demonstrated the highest expression of TRAIL and Fas, whereas in adenomas it was increased compared to normal tissue, but lower than in hyperplasias. These factors could be an additive tool in the differential diagnosis of parathyroid lesions.

  9. 36 CFR 212.55 - Criteria for designation of roads, trails, and areas.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... roads, trails, and areas. 212.55 Section 212.55 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE TRAVEL MANAGEMENT Designation of Roads, Trails, and Areas for Motor Vehicle Use § 212.55 Criteria for designation of roads, trails, and areas. (a) General criteria for designation of...

  10. 7. Roaring Fork Motor Nature Trail, rocks along edge of ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. Roaring Fork Motor Nature Trail, rocks along edge of road. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  11. 30 CFR 75.830 - Splicing and repair of trailing cables.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... high-voltage trailing cable within 35 feet of the continuous mining machine is prohibited. (2) Only four (4) splices will be allowed at any one time for the portion of the trailing cable that extends...

  12. 30 CFR 75.830 - Splicing and repair of trailing cables.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... high-voltage trailing cable within 35 feet of the continuous mining machine is prohibited. (2) Only four (4) splices will be allowed at any one time for the portion of the trailing cable that extends...

  13. 2. Roaring Fork Motor Nature Trail, road view before first ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. Roaring Fork Motor Nature Trail, road view before first stop. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  14. 6. Roaring Fork Motor Nature Trail, road view after stop ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. Roaring Fork Motor Nature Trail, road view after stop four. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  15. Skills in Motion: Boys' Trail Motorbiking Activities as Transitions into Working-Class Masculinity in a Post-Industrial Locale

    ERIC Educational Resources Information Center

    Ivinson, Gabrielle Mary

    2014-01-01

    During an ethnographic research project exploring young people's perceptions of living in a post-industrial semi-rural place, boys aged 13/14 years revealed their semi-clandestine motorbiking activities across mountains trails. It was found that riding motorbikes and fixing engines were potential resources for young boys' transitions into adult…

  16. Approach for Structurally Clearing an Adaptive Compliant Trailing Edge Flap for Flight

    NASA Technical Reports Server (NTRS)

    Miller, Eric J.; Lokos, William A.; Cruz, Josue; Crampton, Glen; Stephens, Craig A.; Kota, Sridhar; Ervin, Gregory; Flick, Pete

    2015-01-01

    The Adaptive Compliant Trailing Edge (ACTE) flap was flown on the NASA Gulfstream GIII test bed at the NASA Armstrong Flight Research Center. This smoothly curving flap replaced the existing Fowler flaps creating a seamless control surface. This compliant structure, developed by FlexSys Inc. in partnership with Air Force Research Laboratory, supported NASA objectives for airframe structural noise reduction, aerodynamic efficiency, and wing weight reduction through gust load alleviation. A thorough structures airworthiness approach was developed to move this project safely to flight.

  17. 3. Roaring Fork Motor Nature Trail, view between second and ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. Roaring Fork Motor Nature Trail, view between second and third stops - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  18. 11. Roaring Fork Motor Nature Trail, boulders along road after ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    11. Roaring Fork Motor Nature Trail, boulders along road after stop 13. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  19. Chalcones Enhance TRAIL-Induced Apoptosis in Prostate Cancer Cells

    PubMed Central

    Szliszka, Ewelina; Czuba, Zenon P; Mazur, Bogdan; Sedek, Lukasz; Paradysz, Andrzej; Krol, Wojciech

    2009-01-01

    Chalcones exhibit chemopreventive and antitumor effects. Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a naturally occurring anticancer agent that induces apoptosis in cancer cells and is not toxic to normal cells. We examined the cytotoxic and apoptotic effect of five chalcones in combination with TRAIL on prostate cancer cells. The cytotoxicity was evaluated by the MTT and LDH assays. The apoptosis was determined using flow cytometry with annexin V-FITC. Our study showed that all five tested chalcones: chalcone, licochalcone-A, isobavachalcone, xanthohumol, butein markedly augmented TRAIL-mediated apoptosis and cytotoxicity in prostate cancer cells and confirmed the significant role of chalcones in chemoprevention of prostate cancer. PMID:20161998

  20. 27. YCC CREW THAT REBUILT PIMA POINT TRAILS. TIM BEALE, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    27. YCC CREW THAT REBUILT PIMA POINT TRAILS. TIM BEALE, NPS TRAILS, FRONT ROW RIGHT; BERNIE PONYAH, NPS YCC SUPERVISOR, BACK ROW RIGHT. - West Rim Drive, Between Grand Canyon Village & Hermit Rest, Grand Canyon, Coconino County, AZ

  1. Center determination for trailed sources in astronomical observation images

    NASA Astrophysics Data System (ADS)

    Du, Jun Ju; Hu, Shao Ming; Chen, Xu; Guo, Di Fu

    2014-11-01

    Images with trailed sources can be obtained when observing near-Earth objects, such as small astroids, space debris, major planets and their satellites, no matter the telescopes track on sidereal speed or the speed of target. The low centering accuracy of these trailed sources is one of the most important sources of the astrometric uncertainty, but how to determine the central positions of the trailed sources accurately remains a significant challenge to image processing techniques, especially in the study of faint or fast moving objects. According to the conditions of one-meter telescope at Weihai Observatory of Shandong University, moment and point-spread-function (PSF) fitting were chosen to develop the image processing pipeline for space debris. The principles and the implementations of both two methods are introduced in this paper. And some simulated images containing trailed sources are analyzed with each technique. The results show that two methods are comparable to obtain the accurate central positions of trailed sources when the signal to noise (SNR) is high. But moment tends to fail for the objects with low SNR. Compared with moment, PSF fitting seems to be more robust and versatile. However, PSF fitting is quite time-consuming. Therefore, if there are enough bright stars in the field, or the high astronometric accuracy is not necessary, moment is competent. Otherwise, the combination of moment and PSF fitting is recommended.

  2. Site of secretion of the trail marker of the eastern tent caterpillar.

    PubMed

    Fitzgerald, T D; Edgerly, J S

    1982-01-01

    A new site of secretion of a chemical trail marker was found on the sternum at the tip of the last abdominal segment of the larva of the eastern tent caterpillarMalacosoma americanum. Larvae marked from this site by drawing their sterna along the substrate when they extended existing trails in search of food and again when they established recruitment trails to food-finds. Differences in the quantity or quality of the marker deposited by exploring and recruiting caterpillars may account for the greater activity of the recruitment trails.

  3. Hip morphology in elite golfers: asymmetry between lead and trail hips.

    PubMed

    Dickenson, Edward; O'Connor, Philip; Robinson, Philip; Campbell, Robert; Ahmed, Imran; Fernandez, Miguel; Hawkes, Roger; Charles, Hutchinson; Griffin, Damian

    2016-09-01

    During a golf swing, the lead hip (left hip in a right-handed player) rotates rapidly from external to internal rotation, while the opposite occurs in the trail hip. This study assessed the morphology and pathology of golfers' hips comparing lead and trail hips. A cohort of elite golfers were invited to undergo MRI of their hips. Hip morphology was evaluated by measuring acetabular depth (pincer shape=negative measure), femoral neck antetorsion (retrotorsion=negative measure) and α angles (cam morphology defined as α angle >55° anteriorly) around the axis of the femoral neck. Consultant musculoskeletal radiologists determined the presence of intra-articular pathology. 55 players (mean age 28 years, 52 left hip lead) underwent MRI. No player had pincer morphology, 2 (3.6%) had femoral retrotorsion and 9 (16%) had cam morphology. 7 trail hips and 2 lead hips had cam morphology (p=0.026). Lead hip femoral neck antetorsion was 16.7° compared with 13.0° in the trail hip (p<0.001). The α angles around the femoral neck were significantly lower in the lead compared with trail hips (p<0.001), with the greatest difference noted in the anterosuperior portion of the head neck junction; 53° vs 58° (p<0.001) and 43° vs 47° (p<0.001). 37% of trail and 16% of lead hips (p=0.038) had labral tears. Golfers' lead and trail hips have different morphology. This is the first time side-to-side asymmetry of cam prevalence has been reported. The trail hip exhibited a higher prevalence of labral tears. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/

  4. Compression Garments, Muscle Contractile Function, and Economy in Trail Runners.

    PubMed

    Vercruyssen, Fabrice; Gruet, Mathieu; Colson, Serge S; Ehrstrom, Sabine; Brisswalter, Jeanick

    2017-01-01

    Physiological mechanisms behind the use of compression garments (CGs) during off-road running are unknown. To investigate the influence of wearing CGs vs conventional running clothing (CON) on muscle contractile function and running economy before and after short-distance trail running. Knee-extensor neuromuscular function and running economy assessed from two 5-min treadmill runs (11 and 14 km/h) were evaluated before and after an 18.6-km short-distance trail run in 12 trained athletes wearing either CGs (stocking + short-tight) or CON. Quadriceps neuromuscular function was assessed from mechanical and EMG recording after maximal percutaneous electrical femoral-nerve stimulations (single-twitch doublets at 10 [Db 10 ] and 100 Hz [Db 100 ] delivered at rest and during maximal quadriceps voluntary contraction [MVC]). Running economy (in mL O 2 · km -1 · kg -1 ) increased after trail running independent of the clothing condition and treadmill speeds (P < .001). Similarly, MVC decreased after CON and CGs conditions (-11% and -13%, respectively, P < .001). For both clothing conditions, a significant decrease in quadriceps voluntary activation, Db 10 , Db 100 , and the low-to-high frequency doublet ratio were observed after trail running (time effect, all P < .01), without any changes in rectus femoris maximal M-wave. Wearing CGs does not reduce physiological alterations induced during short-distance trail running. Further studies should determine whether higher intensity of compression pressure during exercises of longer duration may be effective to induce any physiological benefits in experienced trail runners.

  5. 12. Roaring Fork Motor Nature Trail, place of a thousand ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    12. Roaring Fork Motor Nature Trail, place of a thousand drips, view from road. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  6. Differences in the impacts of formal and informal recreational trails on urban forest loss and tree structure.

    PubMed

    Ballantyne, Mark; Pickering, Catherine Marina

    2015-08-15

    Recreational trails are one of the most common types of infrastructure used for nature-based activities such as hiking and mountain biking worldwide. Depending on their design, location, construction, maintenance and use, these trails differ in their environmental impacts. There are few studies, however, comparing the impacts of different trail types including between formal management-created trails and informal visitor-created trails. Although both types of trails can be found in remote natural areas, dense networks of them often occur in forests close to cities where they experience intense visitor use. To assess the relative impacts of different recreational trails in urban forests, we compared the condition of the trail surface, loss of forest strata and changes in tree structure caused by seven types of trails (total network 46.1 km) traversing 17 remnants of an endangered urban forest in Australia. After mapping and classifying all trails, we assessed their impact on the forest condition at 125 sites (15 sites per trail type, plus 15 control sites within undisturbed forest). On the trail sites, the condition of the trail surface, distance from the trail edge to four forest strata (litter, understory, midstorey and tree cover) and structure of the tree-line were assessed. Informal trails generally had poorer surface conditions and were poorly-designed and located. Per site, formal and informal trails resulted in similar loss of forest strata, with wider trails resulting in greater loss of forest. Because there were more informal trails, however, they accounted for the greatest cumulative forest loss. Structural impacts varied, with the widest informal trails and all formal hardened trails resulting in similar reductions in canopy cover and tree density but an increase in saplings. These structural impacts are likely a function of the unregulated and intense use of large informal trails, and disturbance from the construction and maintenance of formal trails

  7. Cohort Profile Update: the TRacking Adolescents' Individual Lives Survey (TRAILS).

    PubMed

    Oldehinkel, Albertine J; Rosmalen, Judith Gm; Buitelaar, Jan K; Hoek, Hans W; Ormel, Johan; Raven, Dennis; Reijneveld, Sijmen A; Veenstra, René; Verhulst, Frank C; Vollebergh, Wilma Am; Hartman, Catharina A

    2015-02-01

    TRAILS consists of a population cohort (N=2230) and a clinical cohort (N=543), both of which were followed from about age 11 years onwards. To date, the population cohort has been assessed five times over a period of 11 years, with retention rates ranging between 80% and 96%. The clinical cohort has been assessed four times over a period of 8 years, with retention rates ranging between 77% and 85%. Since the IJE published a cohort profile on the TRAILS in 2008, the participants have matured from adolescents into young adults. The focus shifted from parents and school to entry into the labour market and family formation, including offspring. Furthermore, psychiatric diagnostic interviews were administered, the database was linked to a Psychiatric Case Registry, and the availability of genome-wide SNP variations opened the door to genome-wide association studies regarding a wide range of (endo)phenotypes. With some delay, TRAILS data are available to researchers outside the TRAILS consortium without costs; access can be obtained by submitting a publication proposal (see www.trails.nl). © The Author 2014; all rights reserved. Published by Oxford University Press on behalf of the International Epidemiological Association.

  8. Modeling and Evaluation of Miles-in-Trail Restrictions in the National Air Space

    NASA Technical Reports Server (NTRS)

    Grabbe, Shon; Sridhar, Banavar

    2003-01-01

    Miles-in-trail restrictions impact flights in the national air space on a daily basis and these restrictions routinely propagate between adjacent Air Route Traffic Control Centers. Since overly restrictive or ineffective miles-in-trail restrictions can reduce the overall efficiency of the national air space, decision support capabilities that model miles-in-trail restrictions should prove to be very beneficial. This paper presents both an analytical formulation and a linear programming approach for modeling the effects of miles-in-trail restrictions. A methodology for monitoring the conformance of an existing miles-in-trail restriction is also presented. These capabilities have been implemented in the Future ATM Concepts Evaluation Tool for testing purposes. To allow alternative restrictions to be evaluated in post-operations, a new mode of operation, which is referred to as the hybrid-playback mode, has been implemented in the simulation environment. To demonstrate the capabilities of these new algorithms, the miles-in-trail restrictions, which were in effect on June 27, 2002 in the New York Terminal Radar Approach Control, are examined. Results from the miles-in-trail conformance monitoring functionality are presented for the ELIOT, PARKE and WHITE departure fixes. In addition, the miles-in-trail algorithms are used to assess the impact of alternative restrictions at the PARKE departure fix.

  9. Combinatorial treatment with anacardic acid followed by TRAIL augments induction of apoptosis in TRAIL resistant cancer cells by the regulation of p53, MAPK and NFκβ pathways.

    PubMed

    Harsha Raj, M; Yashaswini, B; Rössler, Jochen; Salimath, Bharathi P

    2016-05-01

    TRAIL, an apoptosis inducing cytokine currently in phase II clinical trial, was investigated for its capability to induce apoptosis in six different human tumor cell lines out of which three cell lines showed resistance to TRAIL induced apoptosis. To investigate whether Anacardic acid (A1) an active component of Anacardium occidentale can sensitize the resistant cell lines to TRAIL induced apoptosis, we treated the resistant cells with suboptimal concentration of A1 and showed that it is a potent enhancer of TRAIL induced apoptosis which up-regulates the expression of both DR4 and DR5 receptors, which has been observed in the cellular, protein and mRNA levels. The death receptors upregulation consequent to A1 treatment was corroborated by the activation of p53 as well as phosphorylation of p38 and JNK MAP kinases and concomitant inactivation of NFκβ and ERK signaling cascades. Also, A1 modulated the expression of key apoptotic players like Bax, Bcl-2 and CAD along with the abatement of tumor angiogenesis in vivo in EAT mouse model. Thus, post A1 treatment the TRAIL resistant cells turned into TRAIL sensitive cells. Hence our results demonstrate that A1 can synergize TRAIL induced apoptosis through the upregulation of death receptors and downregulation of anti-apoptotic proteins in cancer context.

  10. The influence of use-related, environmental, and managerial factors on soil loss from recreational trails.

    PubMed

    Olive, Nathaniel D; Marion, Jeffrey L

    2009-03-01

    Recreational uses of unsurfaced trails inevitably result in their degradation, with the type and extent of resource impact influenced by factors such as soil texture, topography, climate, trail design and maintenance, and type and amount of use. Of particular concern, the loss of soil through erosion is generally considered a significant and irreversible form of trail impact. This research investigated the influence of several use-related, environmental, and managerial factors on soil loss on recreational trails and roads at Big South Fork National River and Recreation Area, a unit of the U.S. National Park Service. Regression modeling revealed that trail position, trail slope alignment angle, grade, water drainage, and type of use are significant determinants of soil loss. The introduction of individual and groups of variables into a series of regression models provides improved understanding and insights regarding the relative influence of these variables, informing the selection of more effective trail management actions. Study results suggest that trail erosion can be minimized by avoiding "fall-line" alignments, steep grades, and valley-bottom alignments near streams, installing and maintaining adequate densities of tread drainage features, applying gravel to harden treads, and reducing horse and all-terrain vehicle use or restricting them to more resistant routes. This research also sought to develop a more efficient Variable Cross-Sectional Area method for assessing soil loss on trails. This method permitted incorporation of CSA measures in a representative sampling scheme applied to a large (24%) sample of the park's 526 km trail system. The variety of soil loss measures derived from the Variable CSA method, including extrapolated trail-wide soil loss estimates, permit an objective quantification of soil erosion on recreational trails and roads. Such data support relational analyses to increase understanding of trail degradation, and long-term monitoring of

  11. The influence of use-related, environmental, and managerial factors on soil loss from recreational trails

    USGS Publications Warehouse

    Olive, Nathaniel D.; Marion, Jeffrey L.

    2009-01-01

    Recreational uses of unsurfaced trails inevitably result in their degradation, with the type and extent of resource impact influenced by factors such as soil texture, topography, climate, trail design and maintenance, and type and amount of use. Of particular concern, the loss of soil through erosion is generally considered a significant and irreversible form of trail impact. This research investigated the influence of several use-related, environmental, and managerial factors on soil loss on recreational trails and roads at Big South Fork National River and Recreation Area, a unit of the U.S. National Park Service. Regression modeling revealed that trail position, trail slope alignment angle, grade, water drainage, and type of use are significant determinants of soil loss. The introduction of individual and groups of variables into a series of regression models provides improved understanding and insights regarding the relative influence of these variables, informing the selection of more effective trail management actions. Study results suggest that trail erosion can be minimized by avoiding “fall-line” alignments, steep grades, and valley-bottom alignments near streams, installing and maintaining adequate densities of tread drainage features, applying gravel to harden treads, and reducing horse and all-terrain vehicle use or restricting them to more resistant routes.This research also sought to develop a more efficient Variable Cross-Sectional Area method for assessing soil loss on trails. This method permitted incorporation of CSA measures in a representative sampling scheme applied to a large (24%) sample of the park's 526 km trail system. The variety of soil loss measures derived from the Variable CSA method, including extrapolated trail-wide soil loss estimates, permit an objective quantification of soil erosion on recreational trails and roads. Such data support relational analyses to increase understanding of trail degradation, and long

  12. Role of trail pheromone in foraging and processionary behavior of pine processionary caterpillars Thaumetopoea pityocampa.

    PubMed

    Fitzgerald, T D

    2003-03-01

    Although caterpillars of Thaumetopoea pityocamnpa may mark their pathways with silk, this study shows that the material is essential to neither the elicitation nor maintenance of trail-following or processionary behavior. Trail following is dependent upon a pheromone the caterpillars deposit by brushing the ventral surfaces of the tips of their abdomens against the substate. Earlier instars are strongly bound to their trail system; in the laboratory, caterpillars followed circular trails continuously for as long as 12 hr before breaking away from them. The trail pheromone is long-lived and soluble in nonpolar solvents, but its volatilization or degradation allows the caterpillars to distinguish new from aged trails. In contrast to trail following, processionary behavior, the head-to-tail, single-file movement of the caterpillars is dependent on neither silk nor the trail pheromone. Stimuli associated with setae found on the tip of the abdomen of the precedent caterpillar serve to hold processions together, and such stimuli take priority over those associated with either the trail pheromone or silk. Although the caterpillars discern trail strength and choose stronger over weaker trails, the trail marking system of the processionary caterpillar appears less sophisticated than those of other, previously studied species of social caterpillars, and colonies are relatively inefficient in abandoning exhausted feeding sites in favor of new food finds. In laboratory studies, females were more likely to lead processions than males, and leaders, regardless of gender, expended more energy in locomotion than followers.

  13. Recreational trails as corridors for alien plants in the Rocky Mountains, USA

    USGS Publications Warehouse

    Wells, Floye H.; Lauenroth, William K.; Bradford, John B.

    2012-01-01

    Alien plant species often use areas of heavy human activity for habitat and dispersal. Roads and utility corridors have been shown to harbor more alien species than the surrounding vegetation and are therefore believed to contribute to alien plant persistence and spread. Recreational trails represent another corridor that could harbor alien species and aid their spread. Effective management of invasive species requires understanding how alien plants are distributed at trailheads and trails and how their dispersal may be influenced by native vegetation. Our overall goal was to investigate the distribution of alien plants at trailheads and trails in the Rocky Mountains of Colorado. At trailheads, we found that although the number of alien species was less than the number of native species, alien plant cover ( x̄=50%) did not differ from native plant cover, and we observed a large number of alien seedlings in the soil seed bank, suggesting that alien plants are a large component of trailhead communities and will continue to be so in the future. Along trails, we found higher alien species richness and cover on trail (as opposed to 4 m from the trail) in 3 out of 4 vegetation types, and we observed higher alien richness and cover in meadows than in other vegetation types. Plant communities at both trailheads and trails, as well as seed banks at trailheads, contain substantial diversity and abundance of alien plants. These results suggest that recreational trails in the Rocky Mountains of Colorado may function as corridors that facilitate the spread of alien species into wildlands. Our results suggest that control of alien plants should begin at trailheads where there are large numbers of aliens and that control efforts on trails should be prioritized by vegetation type.

  14. Comprehensive Trail Making Test

    ERIC Educational Resources Information Center

    Gray, Rebecca

    2006-01-01

    The Comprehensive Trail Making Test (CTMT) is designed to be used in neuropsychological assessment for the purposes of detecting effects of brain defects and deficits and in tracking progress in rehabilitation. More specific purposes include the detection of frontal lobe deficits, problems with psychomotor speed, visual search and sequencing,…

  15. Alaska State Trails Program

    Science.gov Websites

    Natural Resources Logo News and Events Outdoor Recreational Trails Advisory Board recruiting new members Involved Volunteers Employment Advisory Board Partners Friends of Parks Poems in Place About Us Contact Eagle Watch Issues Bid Calendar & Results Heritage Newsletter Advisory Board Meetings Store Parks

  16. Sex pheromone and trail pheromone of the sand termite Psammotermes hybostoma.

    PubMed

    Sillam-Dussès, David; Hanus, Robert; Abd El-Latif, Ashraf Oukasha; Jiroš, Pavel; Krasulová, Jana; Kalinová, Blanka; Valterová, Irena; Sobotník, Jan

    2011-02-01

    Within the complex network of chemical signals used by termites, trail pheromones and sex pheromones are among the best known. Numerous recent papers map the chemical identity and glandular origin of these pheromones in nearly all major isopteran taxa. In this study, we aimed to describe the sex pheromone and the trail pheromone of a poorly known sand termite, Psammotermes hybostoma. We identified (3Z,6Z,8E)-dodeca-3,6,8-trien-1-ol (dodecatrienol) as the sex pheromone released by tergal and sternal glands of female imagos and, at the same time, as the trail pheromone secreted from the sternal gland of workers. We conclude that chemical communication in Psammotermes does not differ from that of most other Rhinotermitidae, such as Reticulitermes, despite the presence of a diterpene as a major component of the trail pheromone of Prorhinotermes to which Psammotermes is presumed to be phylogenetically close. Our findings underline once again the conservative nature of chemical communication in termites, with dodecatrienol being a frequent component of pheromonal signals in trail following and sex attraction and, at the same time, a tight evolutionary relationship between the trail following of working castes and the sex attraction of imagos.

  17. Behavioral evidence for multicomponent trail pheromone in the termite,Reticulitermes flavipes (Kollar) (Isoptera: Rhinotermitidae).

    PubMed

    Runcie, C D

    1987-09-01

    Evidence is presented for a multicomponent trail pheromone in the eastern subterranean termiteReticulitermes flavipes (Kollar). Choice tests were used to compare strength and persistency of trails made by termites in glass tubes. Tubes connected to termite nests for 24 hr were marked by termites with an extremely long-lasting chemical that persisted for at least one year, and a highly volatile substance that decayed in 15 min. Individual termites varied the trail they deposited under different circumstances. When removed from the nest and placed in a clean tube, they deposited a highly volatile substance. They left stronger and more persistent trails when exploring clean tubes attached to the nest. When they discovered a new food source, they left trails that were more attractive and far more persistent than trails made by exploring or displaced termites. A food trail made by 15 workers was still effective after 24 hr, whereas a trail made by 50 displaced workers, walking one after another through a tube, lasted only 5 min.

  18. 14. Roaring Fork Motor Nature Trail, Place of a thousand ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    14. Roaring Fork Motor Nature Trail, Place of a thousand drips, view with three culvert pipes. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  19. On the wake flow of asymmetrically beveled trailing edges

    NASA Astrophysics Data System (ADS)

    Guan, Yaoyi; Pröbsting, Stefan; Stephens, David; Gupta, Abhineet; Morris, Scott C.

    2016-05-01

    Trailing edge and wake flows are of interest for a wide range of applications. Small changes in the design of asymmetrically beveled or semi-rounded trailing edges can result in significant difference in flow features which are relevant for the aerodynamic performance, flow-induced structural vibration and aerodynamically generated sound. The present study describes in detail the flow field characteristics around a family of asymmetrically beveled trailing edges with an enclosed trailing-edge angle of 25° and variable radius of curvature R. The flow fields over the beveled trailing edges are described using data obtained by particle image velocimetry (PIV) experiments. The flow topology for different trailing edges was found to be strongly dependent on the radius of curvature R, with flow separation occurring further downstream as R increases. This variation in the location of flow separation influences the aerodynamic force coefficients, which were evaluated from the PIV data using a control volume approach. Two-point correlations of the in-plane velocity components are considered to assess the structure in the flow field. The analysis shows large-scale coherent motions in the far wake, which are associated with vortex shedding. The wake thickness parameter yf is confirmed as an appropriate length scale to characterize this large-scale roll-up motion in the wake. The development in the very near wake was found to be critically dependent on R. In addition, high-speed PIV measurements provide insight into the spectral characteristics of the turbulent fluctuations. Based on the time-resolved flow field data, the frequency range associated with the shedding of coherent vortex pairs in the wake is identified. By means of time-correlation of the velocity components, turbulent structures are found to convect from the attached or separated shear layers without distinct separation point into the wake.

  20. Rail Trails and Property Values: Is There an Association?

    ERIC Educational Resources Information Center

    Hartenian, Ella; Horton, Nicholas J.

    2015-01-01

    The Rail Trail and Property Values dataset includes information on a set of n = 104 homes which sold in Northampton, Massachusetts in 2007. The dataset provides house information (square footage, acreage, number of bedrooms, etc.), price estimates (from Zillow.com) at four time points, location, distance from a rail trail in the community, biking…

  1. Elk responses to trail-based recreation on public forests

    Treesearch

    Michael J. Wisdom; Haiganoush K. Preisler; Leslie M. Naylor; Robert G. Anthony; Bruce K. Johnson; Mary M. Rowland

    2018-01-01

    Trail-based recreation is a popular use of public forests in the United States, and four types are common: allterrain vehicle (ATV) riding, mountain biking, hiking, and horseback riding. Effects on wildlife, however, are controversial and often a topic of land use debates. Accordingly, we studied trail-based recreation effects on elk (Cervus canadensis...

  2. 76 FR 71601 - Record of Decision, Long Walk National Historic Trail Feasibility Study/Abbreviated Final...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-18

    ... National Historic Trail Feasibility Study/Abbreviated Final Environmental Impact Statement, National Trails... Decision on the Abbreviated Final Environmental Impact Statement for the Long Walk National Historic Trail... Final Environmental Impact Statement for the Long Walk National Historic Trail Feasibility Study...

  3. Assessing and Predicting Erosion from Off Highway Vehicle Trails in Front-Range Rocky Mountain Watersheds.

    NASA Astrophysics Data System (ADS)

    Howard, M. J.; Silins, U.; Anderson, A.

    2016-12-01

    Off highway vehicle (OHV) trails have the potential to deliver sediment to sensitive headwater streams and increased OHV use is a growing watershed management concern in many Rocky Mountain regions. Predictive tools for estimating erosion and sediment inputs are needed to support assessment and management of erosion from OHV trail networks. The objective of this study was to a) assess erodibility (K factor) and total erosion from OHV trail networks in Rocky Mountain watersheds in south-west Alberta, Canada, and to b) evaluate the applicability of the Universal Soil Loss Equation (USLE) for predicting OHV trail erosion to support erosion management strategies. Measured erosion rates and erodibility (K) from rainfall simulation plots on OHV trails during the summers of 2014 and 2015 were compared to USLE predicted erosion from these same trails. Measured erodibility (K) from 23 rainfall simulation plots was highly variable (0.001-0.273 Mg*ha*hr/ha*MJ*mm) as was total seasonal erosion from 52 large trail sections (0.0595-43.3 Mg/ha) across trail segments of variable slope, stoniness, and trail use intensity. In particular, intensity of trail use had a large effect on both erodibility and total erosion that is not presently captured by erodibility indices (K) derived from soil characteristics. Results of this study suggest that while application of USLE for predicting erosion from OHV trail networks may be useful for initial coarse erosion assessment, a better understanding of the effect of factors such as road/trail use intensity on erodibility is needed to support use of USLE or associated erosion prediction tools for road/trail erosion management.

  4. Novel targets for sensitizing breast cancer cells to TRAIL-induced apoptosis with siRNA delivery.

    PubMed

    Thapa, Bindu; Bahadur Kc, Remant; Uludağ, Hasan

    2018-02-01

    Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) induces apoptosis in variety of cancer cells without affecting most normal cells, which makes it a promising agent for cancer therapy. However, TRAIL therapy is clinically not effective due to resistance induction. To identify novel regulators of TRAIL that can aid in therapy, protein targets whose silencing sensitized breast cancer cells against TRAIL were screened with an siRNA library against 446 human apoptosis-related proteins in MDA-231 cells. Using a cationic lipopolymer (PEI-αLA) for delivery of library members, 16 siRNAs were identified that sensitized the TRAIL-induced death in MDA-231 cells. The siRNAs targeting BCL2L12 and SOD1 were further evaluated based on the novelty and their ability to sensitize TRAIL induced cell death. Silencing both targets sensitized TRAIL-mediated cell death in MDA-231 cells as well as TRAIL resistant breast cancer cells, MCF-7. Combination of TRAIL and siRNA silencing BCL2L12 had no effect in normal human umbilical vein cells and human bone marrow stromal cell. The silencing of BCL2L12 and SOD1 enhanced TRAIL-mediated apoptosis in MDA-231 cells via synergistically activating capsase-3 activity. Hence, here we report siRNAs targeting BCL2L12 and SOD1 as a novel regulator of TRAIL-induced cell death in breast cancer cells, providing a new approach for enhancing TRAIL therapy for breast cancer. The combination of siRNA targeting BCL2L12 and TRAIL can be a highly effective synergistic pair in breast cancer cells with minimal effect on the non-transformed cells. © 2017 UICC.

  5. Near Field Trailing Edge Tone Noise Computation

    NASA Technical Reports Server (NTRS)

    Loh, Ching Y.

    2002-01-01

    Blunt trailing edges in a flow often generate tone noise due to wall-jet shear layer and vortex shedding. In this paper, the space-time conservation element (CE/SE) method is employed to numerically study the near-field noise of blunt trailing edges. Two typical cases, namely, flow past a circular cylinder (aeolian noise problem) and flow past a flat plate of finite thickness are considered. The computed frequencies compare well with experimental data. For the aeolian noise problem, comparisons with the results of other numerical approaches are also presented.

  6. Pharmacophore reassignment for induction of the immunosurveillance cytokine TRAIL.

    PubMed

    Jacob, Nicholas T; Lockner, Jonathan W; Kravchenko, Vladimir V; Janda, Kim D

    2014-06-23

    Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) is an immunosurveillance cytokine that kills cancer cells but demonstrates little toxicity against normal cells. While investigating the TRAIL-inducing imidazolinopyrimidinone TIC10, a misassignment of its active structure was uncovered. Syntheses of the two isomers, corresponding to the published and reassigned structures, are reported. The ability of each to induce TRAIL expression in macrophages was investigated and it was found that only the compound corresponding to the reassigned structure shows the originally reported activity; the compound corresponding to the published structure is inactive. Importantly, this structural reassignment has furnished a previously unknown antitumor pharmacophore. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  7. Rail-trails and special events: community and economic benefits

    Treesearch

    Charles Nelson; Christine Vogt; Joel Lynch; Daniel Stynes

    2002-01-01

    This paper discusses community and economic benefits associated with two recreational bicycle special events held on the Pere Marquette Rail-Trail (PMRT) in Midland County Michigan during the summer of 1999. One event was an annual ride to fundraise for the Rails to Trails Conservancy of Michigan. Approximately 1,800 participants rode in the event which included two...

  8. Bio-Inspired Control of Roughness and Trailing Edge Noise

    NASA Astrophysics Data System (ADS)

    Clark, Ian Andrew

    Noise from fluid flow over rough surfaces is an important consideration in the design and performance of certain vehicles with high surface-area-to-perimeter ratios. A new method of noise control based on the anatomy of owls is developed and consists of fabric or fibrous canopies suspended above the surface. The method is tested experimentally and is found to reduce the total far-field noise emitted by the surface. The treatment also is found to reduce the magnitude of pressure fluctuations felt by the underlying surface by up to three orders of magnitude. Experimental investigations into the effects of geometric parameters of the canopies lead to an optimized design which maximizes noise reduction. The results obtained during the canopy experiment inspired a separate new device for the reduction of trailing edge noise. This type of noise is generated by flow past the wing of an aircraft or the blades of a wind turbine, and is a source of annoyance for those in surrounding communities. The newly developed treatment consists of small fins, or "finlets," placed near the trailing edge of an airfoil. The treatment is tested experimentally at near-full-scale conditions and is found to reduce the magnitude of far-field noise by up to 10 dB. Geometric parameters of the finlets are tested to determine the optimal size and spacing of the finlets to maximize noise reduction. Follow-up computational and experimental studies reveal the fluid mechanics behind the noise reduction by showing that the finlets produce a velocity deficit in the flow near the trailing edge and limit the magnitude and spanwise correlation lengthscale of turbulence near the trailing edge, factors which determine the magnitude of far-field noise. In a final experiment, the finlets are applied to a marine propeller and are found to reduce not only trailing edge noise, but also noise caused by the bluntness of the trailing edge. The results of this experiment show the potential usefulness of finlets to

  9. 77 FR 1723 - Notice of Availability, Potomac Heritage National Scenic Trail

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-11

    ... planned Trail segments for non-motorized travel. Communities have invested in the Trail concept for a... transportation, education and/or heritage tourism. The Foundation assembles in one document decisions and...

  10. Geomorphological hazard and tourist vulnerability along Portofino Park trails (Italy)

    NASA Astrophysics Data System (ADS)

    Brandolini, P.; Faccini, F.; Piccazzo, M.

    2006-06-01

    The many trails existing in the coastal area of Portofino Promontory are used by tourists for trekking or as pathways to small villages and beaches. The aim of this paper is to define geomorphological hazard and tourist vulnerability in this area, within the framework of the management and planning of hiking activities in Portofino Natural Park. In particular, processes triggered by gravity, running waters and wave motion, affecting the slopes and the cliff, are considered. The typology of the trails and trail maintenance are also taken into account in relation to weather conditions that can make the excursion routes dangerous for tourists. In conclusion, an operative model is applied for the definition of possible risk scenarios. This model is founded on an inventory and the quantification of geomorphological hazards and tourist vulnerability, in comparison with trail rescue data. The model can be applied to other environments and tourist areas.

  11. Immunoglobulin Fc domain fusion to TRAIL significantly prolongs its plasma half-life and enhances its antitumor activity.

    PubMed

    Wang, Haizhen; Davis, Jennifer S; Wu, Xiangwei

    2014-03-01

    TRAIL (Apo2L) is a potent inducer of cell death. Interest in TRAIL has increased, following the observation that TRAIL can selectively kill a wide variety of human cancer cells without killing normal cells both in vitro and when grown as xenografts. Therefore, TRAIL has been proposed as a promising anticancer agent and currently is being tested in clinical trials. However, recombinant TRAIL has a very short plasma half-life, which limits its therapeutic potential. To overcome this limitation, we investigated the ability of the human IgG1 fragment crystallizable region (Fc) to enhance TRAIL stability. In this report, we show that Fc-TRAIL chimeric protein displays higher specific activity in vitro and a significantly longer half-life in mice than recombinant human TRAIL (rh-TRAIL). No short-term toxicity, especially liver toxicity, was observed. More importantly, Fc-TRAIL was much more effective in inhibiting tumor growth in a xenograft tumor model compared with rh-TRAIL. Our data suggest that fusion of Fc to TRAIL is able to improve the bioavailability and activity of TRAIL both in vitro and in vivo, and Fc-TRAIL may be explored for future clinical applications in cancer treatment and prevention. ©2014 AACR.

  12. Monitoring of recreational trail erosion using terrestrial structure-from-motion approach

    NASA Astrophysics Data System (ADS)

    Ewertowski, Marek; Tomczyk, Aleksandra

    2017-04-01

    Protected natural areas (PNAs) such as national and landscape parks as well as suburban green areas often constitute areas very popular among the visitors. Visitor pressure in PNAs is focused mainly on recreational trails, which facilitate activities such as hiking, cycling, horse riding. Trails prepared for different groups of users are among the most common types of infrastructure in PNAs, facilitating access to these areas. However, high visitor pressure can lead to increases in trail width and an associated increase in soil erosion. In case of extensive protected areas, the performing of regular geodetic monitoring using dGPS or laser scanning is expensive and therefore park managers often face a problem in selecting sites for impact monitoring. However, recent advances in technology enables the development of low-cost alternatives for traditional surveys. Consumer-grade cameras can be used to rapid acquire of photographs. The ground-based photographs can be subsequently processed through the structure-from-motion approach to generate detailed mosaics and digital elevation models of trail surfaces. It is possible to apply such models to study, monitor and quantify processes like soil erosion and vegetation trampling. In this study, we present methodological framework for monitoring of trail impact with the use of structure-from-motion approach and demonstrate its application based on examples from recreational trail located in suburban settings of Poznań. The data were collected on 10-meter long trail segment in June, July and October 2016 capturing the initial condition at the beginning of the months, and then two session pre-, and immediately after intense rainfall event, and the last session after termination of summer season. The total number of images was between 150 and 300 for each of the survey session. Dens point clouds were from 18 to 29 million points and were downsampled to DEM with 1 mm resolution. To detect surface changes, Digital elevation

  13. Characterizing near-surface CO2 conditions before injection - Perspectives from a CCS project in the Illinois Basin, USA

    USGS Publications Warehouse

    Locke, R.A.; Krapac, I.G.; Lewicki, J.L.; Curtis-Robinson, E.

    2011-01-01

    The Midwest Geological Sequestration Consortium is conducting a large-scale carbon capture and storage (CCS) project in Decatur, Illinois, USA to demonstrate the ability of a deep saline formation to store one million tonnes of carbon dioxide (CO2) from an ethanol facility. Beginning in early 2011, CO2 will be injected at a rate of 1,000 tonnes/day for three years into the Mount Simon Sandstone at a depth of approximately 2,100 meters. An extensive Monitoring, Verification, and Accounting (MVA) program has been undertaken for the Illinois Basin Decatur Project (IBDP) and is focused on the 0.65 km2 project site. Goals include establishing baseline conditions to evaluate potential impacts from CO2 injection, demonstrating that project activities are protective of human health and the environment, and providing an accurate accounting of stored CO2. MVA efforts are being conducted pre-, during, and post- CO2 injection. Soil and net CO2 flux monitoring has been conducted for more than one year to characterize near-surface CO2 conditions. More than 2,200 soil CO2 flux measurements have been manually collected from a network of 118 soil rings since June 2009. Three ring types have been evaluated to determine which type may be the most effective in detecting potential CO 2 leakage. Bare soil, shallow-depth rings were driven 8 cm into the ground and were prepared to minimize surface vegetation in and near the rings. Bare soil, deep-depth rings were prepared similarly, but were driven 46 cm. Natural-vegetation, shallow-depth rings were driven 8 cm and are most representative of typical vegetation conditions. Bare-soil, shallow-depth rings had the smallest observed mean flux (1.78 ??mol m-2 s-1) versus natural-vegetation, shallow-depth rings (3.38 ??mol m-2 s-1). Current data suggest bare ring types would be more sensitive to small CO2 leak signatures than natural ring types because of higher signal to noise ratios. An eddy covariance (EC) system has been in use since June

  14. 49 CFR 230.108 - Steam locomotive leading and trailing trucks.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false Steam locomotive leading and trailing trucks. 230.108 Section 230.108 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL... and trailing trucks. (a) Maintenance. Trucks shall be maintained in safe and suitable condition for...

  15. View of blind curve along National Old Trails Road, about ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    View of blind curve along National Old Trails Road, about 1,000 feet from top, facing north. - La Bajada Historic Trails and Roads, Approximately 1 mile East/Northeast of intersection of State Highway 16 and Indian Service Road 841, La Bajada, Santa Fe County, NM

  16. Chemoorientation of eastern tent caterpillars to trail pheromone, 5β-Cholestane-3,24-dione.

    PubMed

    Peterson, S C; Fitzgerald, T D

    1991-10-01

    Chemoorientation behavior of the larval eastern tent caterpillar,Malacosoma americanum, was studied using the synthetic trail pheromone 5β-cholestane-3,24-dione. Divergent arms of Y mazes were treated with various concentration ratios of the pheromone. At application rates of 10(-10)-10(-9) g/mm of trail, larvae showed a significant preference for stronger trails when concentration ratios differed by as little as 4:1. At application rates of 10(-8) and greater there was no significant difference in trail choice even when trails differed in strength by a full order of magnitude. Other studies showed that the caterpillars abandon the pattern of choosing stronger over weaker trails when they repeatedly fail to find food at the end of a stronger trail. Experiments in which larvae were required to choose trails separated by a gap demonstrated orientation by chemoklinotaxis. Caterpillars that had one of the maxillary palps ablated looped in the direction of their intact chemo-receptor when placed on filter paper treated uniformly with pheromone, indicating that they may also orient by tropotaxis. The relevance of these findings to the tent caterpillar communication system is discussed.

  17. Vortex coupling in trailing vortex-wing interactions

    NASA Astrophysics Data System (ADS)

    Chen, C.; Wang, Z.; Gursul, I.

    2018-03-01

    The interaction of trailing vortices of an upstream wing with rigid and flexible downstream wings has been investigated experimentally in a wind tunnel, using particle image velocimetry, hot-wire, force, and deformation measurements. Counter-rotating upstream vortices exhibit increased meandering when they are close to the tip of the downstream wing. The upstream vortex forms a pair with the vortex shed from the downstream wing and then exhibits large displacements around the wing tip. This coupled motion of the pair has been found to cause large lift fluctuations on the downstream wing. The meandering of the vortex pair occurs at the natural meandering frequency of the isolated vortex, with a low Strouhal number, and is not affected by the frequency of the large-amplitude wing oscillations if the downstream wing is flexible. The displacement of the leading vortex is larger than that of the trailing vortex; however, it causes highly correlated variations of the core radius, core vorticity, and circulation of the trailing vortex with the coupled meandering motion. In contrast, co-rotating vortices do not exhibit any increased meandering.

  18. Assessing and Understanding Trail Degradation: Results from Big South Fork National River and Recreational Area

    USGS Publications Warehouse

    Marion, J.L.; Olive, N.

    2006-01-01

    This report describes results from a comprehensive assessment of resource conditions on a large (24%) sample of the trail system within Big South Fork National River and Recreational Area (BSF). Components include research to develop state-of-knowledge trail impact assessment and monitoring methods, application of survey methods to BSF trails, analysis and summary of results, and recommendations for trail management decision making and future monitoring. Findings reveal a trail system with some substantial degradation, particularly soil erosion, which additionally threatens water quality in areas adjacent to streams and rivers. Factors that contribute to or influence these problems are analyzed and described. Principal among these are trail design factors (trail topographic position, soil texture, grade and slope alignment angle), use-related factors (type and amount of use), and maintenance factors (water drainage). Recommendations are offered to assist managers in improving the sustainability of the trails system to accommodate visitation while enhancing natural resource protection.

  19. Wall Modeled Large Eddy Simulation of Airfoil Trailing Edge Noise

    NASA Astrophysics Data System (ADS)

    Kocheemoolayil, Joseph; Lele, Sanjiva

    2014-11-01

    Large eddy simulation (LES) of airfoil trailing edge noise has largely been restricted to low Reynolds numbers due to prohibitive computational cost. Wall modeled LES (WMLES) is a computationally cheaper alternative that makes full-scale Reynolds numbers relevant to large wind turbines accessible. A systematic investigation of trailing edge noise prediction using WMLES is conducted. Detailed comparisons are made with experimental data. The stress boundary condition from a wall model does not constrain the fluctuating velocity to vanish at the wall. This limitation has profound implications for trailing edge noise prediction. The simulation over-predicts the intensity of fluctuating wall pressure and far-field noise. An improved wall model formulation that minimizes the over-prediction of fluctuating wall pressure is proposed and carefully validated. The flow configurations chosen for the study are from the workshop on benchmark problems for airframe noise computations. The large eddy simulation database is used to examine the adequacy of scaling laws that quantify the dependence of trailing edge noise on Mach number, Reynolds number and angle of attack. Simplifying assumptions invoked in engineering approaches towards predicting trailing edge noise are critically evaluated. We gratefully acknowledge financial support from GE Global Research and thank Cascade Technologies Inc. for providing access to their massively-parallel large eddy simulation framework.

  20. Ho-Nee-Um Trail.

    ERIC Educational Resources Information Center

    Irwin, Harriet; And Others

    Appreciation and concern for the preservation of our natural resources by all citizens is the primary concern of this teacher's guide for use in the elementary grades. It employes the use of a filmstrip in conjunction with a local nature trail, to guide students in developing awareness - by looking closely, listening, touching, and smelling. Major…

  1. Tracking Online Trails

    NASA Astrophysics Data System (ADS)

    Qi, Man; Edgar-Nevill, Denis; Wang, Yongquan; Xu, Rongsheng

    Traceability is a key to the investigation of the internet criminal and a cornerstone of internet research. It is impossible to prevent all internet misuse but may be possible to identify and trace the users, and then take appropriate action. This paper presents the value of traceability within the email/-newsposting utilities, the technologies being using to hide identities, the difficulties in locating the traceable data and the challenges in tracking online trails.

  2. Dynamics of foraging trails in the Neotropical termite Velocitermes heteropterus (Isoptera: Termitidae).

    PubMed

    Haifig, Ives; Jost, Christian; Fourcassié, Vincent; Zana, Yossi; Costa-Leonardo, Ana Maria

    2015-09-01

    Foraging behavior in termites varies with the feeding habits of each species but often occurs through the formation of well-defined trails that connect the nest to food sources in species that build structured nests. We studied the formation of foraging trails and the change in caste ratio during foraging in the termite Velocitermes heteropterus. This species is widespread in Cerrado vegetation where it builds epigeal nests and forages in open-air at night. Our aim was to understand the processes involved in the formation of foraging trails, from the exploration of new unmarked areas to the recruitment of individuals to food and the stabilization of traffic on the trails, as well as the participation of the different castes during these processes. Foraging trails were videotaped in the laboratory and the videos were then analyzed both manually and automatically to assess the flow of individuals and the caste ratio on the trails as well as to examine the spatial organization of traffic over time. Foraging trails were composed of minor workers, major workers, and soldiers. The flow of individuals on the trails gradually increased from the beginning of the exploration of new areas up to the discovery of the food. The caste ratio remained constant throughout the foraging excursion: major workers, minor workers and soldiers forage in a ratio of 8:1:1, respectively. The speed of individuals was significantly different among castes, with major workers and soldiers being significantly faster than minor workers. Overall, our results show that foraging excursions in V. heteropterus may be divided in three different phases, characterized by individual speeds, differential flows and lane segregation. Copyright © 2015 Elsevier B.V. All rights reserved.

  3. On the Trail to Fitness.

    ERIC Educational Resources Information Center

    American School and University, 1979

    1979-01-01

    The University of Hartford planned fitness trail will allow students to develop their bodies by providing a jogging route to improve cardiovascular fitness and exercise stations designed to provide warm-up exercises and improve strength, flexibility, balance, and endurance. (Author/MLF)

  4. 75 FR 19646 - Cheniere Creole Trail Pipeline, L.P.; Notice of Request Under Blanket Authorization

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-15

    ... Creole Trail Pipeline, L.P.; Notice of Request Under Blanket Authorization April 7, 2010. Take notice that on April 2, 2010, Cheniere Creole Trail Pipeline, L.P. (Creole Trail), 700 Milam, Suite 800... Trail seeks authorization to construct and operate approximately 550 feet of 12-inch diameter pipe (no...

  5. Relationships between Induced Seismicity and Fluid Injection: Development of Strategies to Manage Injection

    NASA Astrophysics Data System (ADS)

    Eichhubl, Peter; Frohlich, Cliff; Gale, Julia; Olson, Jon; Fan, Zhiqiang; Gono, Valerie

    2014-05-01

    Induced seismicity during or following the subsurface injection of waste fluids such as well stimulation flow back and production fluids has recently received heightened public and industry attention. It is understood that induced seismicity occurs by reactivation of existing faults that are generally present in the injection intervals. We seek to address the question why fluid injection triggers earthquakes in some areas and not in others, with the aim toward improved injection methods that optimize injection volume and cost while avoiding induced seismicity. A GIS database has been built of natural and induced earthquakes in four hydrocarbon-producing basins: the Fort Worth Basin, South Texas, East Texas/Louisiana, and the Williston Basin. These areas are associated with disposal from the Barnett, Eagle Ford, Bakken, and Haynesville Shales respectively. In each region we analyzed data that were been collected using temporary seismographs of the National Science Foundation's USArray Transportable Array. Injection well locations, formations, histories, and volumes are also mapped using public and licensed datasets. Faults are mapped at a range of scales for selected areas that show different levels of seismic activity, and scaling relationships used to extrapolate between the seismic and wellbore scale. Reactivation potential of these faults is assessed using fault occurrence, and in-situ stress conditions, identifying areas of high and low fault reactivation potential. A correlation analysis between fault reactivation potential, induced seismicity, and fluid injection will use spatial statistics to quantify the probability of seismic fault reactivation for a given injection pressure in the studied reservoirs. The limiting conditions inducing fault reactivation will be compared to actual injection parameters (volume, rate, injection duration and frequency) where available. The objective of this project is a statistical reservoir- to basin-scale assessment of fault

  6. Impact of trailing edge shape on the wake and propulsive performance of pitching panels

    NASA Astrophysics Data System (ADS)

    Van Buren, T.; Floryan, D.; Brunner, D.; Senturk, U.; Smits, A. J.

    2017-01-01

    The effects of changing the trailing edge shape on the wake and propulsive performance of a pitching rigid panel are examined experimentally. The panel aspect ratio is AR=1 , and the trailing edges are symmetric chevron shapes with convex and concave orientations of varying degree. Concave trailing edges delay the natural vortex bending and compression of the wake, and the mean streamwise velocity field contains a single jet. Conversely, convex trailing edges promote wake compression and produce a quadfurcated wake with four jets. As the trailing edge shape changes from the most concave to the most convex, the thrust and efficiency increase significantly.

  7. 77 FR 32178 - Notification of Trails Act Agreement/Substitute Sponsorship

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-31

    ... DEPARTMENT OF TRANSPORTATION Surface Transportation Board [STB Ex Parte No. 702] Notification of Trails Act Agreement/Substitute Sponsorship AGENCY: Surface Transportation Board. ACTION: Notice of OMB... Trails System Act and Railroad Rights-of-Way, STB Ex Parte No. 702 (STB served Apr. 30, 2012) (77 FR...

  8. Rejuvenating harm reduction projects for injection drug users: Ukraine's nationwide introduction of peer-driven interventions.

    PubMed

    Smyrnov, Pavlo; Broadhead, Robert S; Datsenko, Oleksandra; Matiyash, Oksana

    2012-03-01

    A peer-driven intervention (PDI) for injecting drug users (IDUs) was implemented in five Ukrainian city-sites to test-pilot its effectiveness in rejuvenating harm reduction (HR) projects that had become moribund. A PDI relies on drug users in a unique way to educate their peers in the community and recruit them for HIV prevention services. The goal of the PDI was to recruit in six month 500 IDUs who had never been respondents before to each of the five HR projects, especially stimulant- and women-injectors, and IDUs<25 years of age. We standardized the PDI's structure and operations across all five sites. All five PDIs were started in May 2007 using a carefully selected handful of "seed" IDU-recruiters who were trained to educate three peers who had never received HR services. We also accessed the database of all five projects and analysed the new respondents they recruited six-months prior to the start-up of the PDIs with the new recruits generated by the PDIs. Whilst the HR projects in the five city-sites recruited 72 new respondents on average during the six months prior to the PDIs' start-up, the PDIs recruited 455 new respondents on average in each city during their six months of operation, indicating that the PDI was 6.3 times more powerful as a recruitment mechanism. Compared to traditional outreach the PDIs resulted in significant increases in the recruitment of women- and young-injectors, and IDUs who injected a more diverse variety of drugs. The PDI can have a rejuvenating effect when added to HR projects that had become stagnate over time, resulting in an increase in the number and diversity of new IDU-respondents. Copyright © 2012 Elsevier B.V. All rights reserved.

  9. Numerical analysis of the impact of permeability on trailing-edge noise

    NASA Astrophysics Data System (ADS)

    Koh, Seong Ryong; Meinke, Matthias; Schröder, Wolfgang

    2018-05-01

    The impact of porous surfaces on the near-wall turbulent structures and the generated trailing-edge noise is analyzed for several trailing-edge shapes of finite thickness using a high resolution large-eddy simulation (LES)/computational aeroacoustics (CAA) method. The porous surface of the trailing edge is defined by the porosity and the viscous permeability determined by the solution of a turbulent flat plate boundary layer at a Reynolds number 1280 based on the displacement thickness in the inflow cross section. The volume-averaged approach for the homogeneous porous medium shows that the porous impedance scales linearly with the porosity and exponentially with the mean structure size of a porous medium. The drag induced by the porous surface changes the friction velocity and the permeability Reynolds number ReK which determines the porous impedance Rs scaled by ReK-2/3. The trailing-edge noise is analyzed for three solid and three porous trailing edges. The effect of a finite span is investigated by the spanwise correlation model based on the measured coherence distribution. The acoustic prediction shows a good agreement with measurements of the broadband spectrum and the strong tone generated by a finite trailing-edge thickness. The pressure gradient inside the porous media is redistributed by the Darcy drag defined by the viscous permeability and the porosity. The mean pressure increases in the upstream direction inside the porous medium such that the flow acceleration involved in the acoustic generation is reduced inside the porous medium. The noise reduction by a porous medium reaches 11 dB for the trailing-edge shape which possesses a sharp corner for the solid surface. The porous surface applied to a semi-circular trailing edge achieves a 4 dB noise reduction. The directivity pattern for individual components of the acoustic spectrum shows that the massive noise reduction is determined at the tone. Enhanced wave diffraction by the thick flat plate changes

  10. Effect of Her-2/neu Signaling on Sensitivity to TRAIL in Prostate Cancer

    DTIC Science & Technology

    2005-06-01

    cytokines (18), and matrix metalloprotease inhibitors (19) are able to render TRAIL-resistant tumor cells sensitive to TRAIL apoptosis. In recent...TRAIL-induced cytotoxicity. As DU-145 cells were treated with acetyl salicylic acid (ASA: aspirin), an inhibitor of IKK , we observed that TRAIL...sulfide (IC50 = 1.02 M for COX-1 and IC50 = 10.43 M for COX-2), NS-398 (a selective COX-2 inhibitor ; IC50 = 4.81 M for COX-1 and IC50 = 0.47 M for COX-2

  11. Action recognition using multi-scale histograms of oriented gradients based depth motion trail Images

    NASA Astrophysics Data System (ADS)

    Wang, Guanxi; Tie, Yun; Qi, Lin

    2017-07-01

    In this paper, we propose a novel approach based on Depth Maps and compute Multi-Scale Histograms of Oriented Gradient (MSHOG) from sequences of depth maps to recognize actions. Each depth frame in a depth video sequence is projected onto three orthogonal Cartesian planes. Under each projection view, the absolute difference between two consecutive projected maps is accumulated through a depth video sequence to form a Depth Map, which is called Depth Motion Trail Images (DMTI). The MSHOG is then computed from the Depth Maps for the representation of an action. In addition, we apply L2-Regularized Collaborative Representation (L2-CRC) to classify actions. We evaluate the proposed approach on MSR Action3D dataset and MSRGesture3D dataset. Promising experimental result demonstrates the effectiveness of our proposed method.

  12. 75 FR 26224 - Cheniere Creole Trail Pipeline, L.P.; Notice of Request Under Blanket Authorization

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-11

    ... Creole Trail Pipeline, L.P.; Notice of Request Under Blanket Authorization May 4, 2010. Take notice that on April 29, 2010, Cheniere Creole Trail Pipeline, L.P. (Creole Trail), 700 Milam, Suite 800, Houston... 157.216(b) of the Commission's regulations under the Natural Gas Act (NGA). Creole Trail seeks...

  13. Trail communication regulated by two trail pheromone components in the fungus-growing termite Odontotermes formosanus (Shiraki).

    PubMed

    Wen, Ping; Ji, Bao-Zhong; Sillam-Dussès, David

    2014-01-01

    The eusocial termites are well accomplished in chemical communication, but how they achieve the communication using trace amount of no more than two pheromone components is mostly unknown. In this study, the foraging process and trail pheromones of the fungus-growing termite Odontotermes formosanus (Shiraki) were systematically studied and monitored in real-time using a combination of techniques, including video analysis, solid-phase microextraction, gas chromatography coupled with either mass spectrometry or an electroantennographic detector, and bioassays. The trail pheromone components in foraging workers were (3Z)-dodec-3-en-1-ol and (3Z,6Z)-dodeca-3,6-dien-1-ol secreted by their sternal glands. Interestingly, ratio of the two components changed according to the behaviors that the termites were displaying. This situation only occurs in termites whereas ratios of pheromone components are fixed and species-specific for other insect cuticular glands. Moreover, in bioassays, the active thresholds of the two components ranged from 1 fg/cm to 10 pg/cm according to the behavioral contexts or the pheromonal exposure of tested workers. The two components did not act in synergy. (3Z)-Dodec-3-en-1-ol induced orientation behavior of termites that explore their environment, whereas (3Z,6Z)-dodeca-3,6-dien-1-ol had both an orientation effect and a recruitment effect when food was discovered. The trail pheromone of O. formosanus was regulated both quantitatively by the increasing number of workers involved in the early phases of foraging process, and qualitatively by the change in ratio of the two pheromone components on sternal glandular cuticle in the food-collecting workers. In bioassays, the responses of workers to the pheromone were also affected by the variation in pheromone concentration and component ratio in the microenvironment. Thus, this termite could exchange more information with nestmates using the traces of the two trail pheromone components that can be easily

  14. Trail Communication Regulated by Two Trail Pheromone Components in the Fungus-Growing Termite Odontotermes formosanus (Shiraki)

    PubMed Central

    Wen, Ping; Ji, Bao-Zhong; Sillam-Dussès, David

    2014-01-01

    The eusocial termites are well accomplished in chemical communication, but how they achieve the communication using trace amount of no more than two pheromone components is mostly unknown. In this study, the foraging process and trail pheromones of the fungus-growing termite Odontotermes formosanus (Shiraki) were systematically studied and monitored in real-time using a combination of techniques, including video analysis, solid-phase microextraction, gas chromatography coupled with either mass spectrometry or an electroantennographic detector, and bioassays. The trail pheromone components in foraging workers were (3Z)-dodec-3-en-1-ol and (3Z,6Z)-dodeca-3,6-dien-1-ol secreted by their sternal glands. Interestingly, ratio of the two components changed according to the behaviors that the termites were displaying. This situation only occurs in termites whereas ratios of pheromone components are fixed and species-specific for other insect cuticular glands. Moreover, in bioassays, the active thresholds of the two components ranged from 1 fg/cm to 10 pg/cm according to the behavioral contexts or the pheromonal exposure of tested workers. The two components did not act in synergy. (3Z)-Dodec-3-en-1-ol induced orientation behavior of termites that explore their environment, whereas (3Z,6Z)-dodeca-3,6-dien-1-ol had both an orientation effect and a recruitment effect when food was discovered. The trail pheromone of O. formosanus was regulated both quantitatively by the increasing number of workers involved in the early phases of foraging process, and qualitatively by the change in ratio of the two pheromone components on sternal glandular cuticle in the food-collecting workers. In bioassays, the responses of workers to the pheromone were also affected by the variation in pheromone concentration and component ratio in the microenvironment. Thus, this termite could exchange more information with nestmates using the traces of the two trail pheromone components that can be easily

  15. Toward the improvement of trail classification in national parks using the recreation opportunity spectrum approach.

    PubMed

    Oishi, Yoshitaka

    2013-06-01

    Trail settings in national parks are essential management tools for improving both ecological conservation efforts and the quality of visitor experiences. This study proposes a plan for the appropriate maintenance of trails in Chubusangaku National Park, Japan, based on the recreation opportunity spectrum (ROS) approach. First, we distributed 452 questionnaires to determine park visitors' preferences for setting a trail (response rate = 68 %). Respondents' preferences were then evaluated according to the following seven parameters: access, remoteness, naturalness, facilities and site management, social encounters, visitor impact, and visitor management. Using nonmetric multidimensional scaling and cluster analysis, the visitors were classified into seven groups. Last, we classified the actual trails according to the visitor questionnaire criteria to examine the discrepancy between visitors' preferences and actual trail settings. The actual trail classification indicated that while most developed trails were located in accessible places, primitive trails were located in remote areas. However, interestingly, two visitor groups seemed to prefer a well-conserved natural environment and, simultaneously, easily accessible trails. This finding does not correspond to a premise of the ROS approach, which supposes that primitive trails should be located in remote areas without ready access. Based on this study's results, we propose that creating trails, which afford visitors the opportunity to experience a well-conserved natural environment in accessible areas is a useful means to provide visitors with diverse recreation opportunities. The process of data collection and analysis in this study can be one approach to produce ROS maps for providing visitors with recreational opportunities of greater diversity and higher quality.

  16. Analysis of the impact of recreational trail usage for prioritising management decisions: a regression tree approach

    NASA Astrophysics Data System (ADS)

    Tomczyk, Aleksandra; Ewertowski, Marek; White, Piran; Kasprzak, Leszek

    2016-04-01

    The dual role of many Protected Natural Areas in providing benefits for both conservation and recreation poses challenges for management. Although recreation-based damage to ecosystems can occur very quickly, restoration can take many years. The protection of conservation interests at the same as providing for recreation requires decisions to be made about how to prioritise and direct management actions. Trails are commonly used to divert visitors from the most important areas of a site, but high visitor pressure can lead to increases in trail width and a concomitant increase in soil erosion. Here we use detailed field data on condition of recreational trails in Gorce National Park, Poland, as the basis for a regression tree analysis to determine the factors influencing trail deterioration, and link specific trail impacts with environmental, use related and managerial factors. We distinguished 12 types of trails, characterised by four levels of degradation: (1) trails with an acceptable level of degradation; (2) threatened trails; (3) damaged trails; and (4) heavily damaged trails. Damaged trails were the most vulnerable of all trails and should be prioritised for appropriate conservation and restoration. We also proposed five types of monitoring of recreational trail conditions: (1) rapid inventory of negative impacts; (2) monitoring visitor numbers and variation in type of use; (3) change-oriented monitoring focusing on sections of trail which were subjected to changes in type or level of use or subjected to extreme weather events; (4) monitoring of dynamics of trail conditions; and (5) full assessment of trail conditions, to be carried out every 10-15 years. The application of the proposed framework can enhance the ability of Park managers to prioritise their trail management activities, enhancing trail conditions and visitor safety, while minimising adverse impacts on the conservation value of the ecosystem. A.M.T. was supported by the Polish Ministry of

  17. Validation of Walking Trails for the Urban TrainingTM of Chronic Obstructive Pulmonary Disease Patients

    PubMed Central

    Arbillaga-Etxarri, Ane; Torrent-Pallicer, Jaume; Gimeno-Santos, Elena; Barberan-Garcia, Anael; Delgado, Anna; Balcells, Eva; Rodríguez, Diego A.; Vilaró, Jordi; Vall-Casas, Pere; Irurtia, Alfredo; Rodriguez-Roisin, Robert; Garcia-Aymerich, Judith

    2016-01-01

    Purpose Accessible interventions to train patients with chronic obstructive pulmonary disease (COPD) are needed. We designed urban trails of different intensities (low, moderate and high) in different types of public spaces (boulevard, beach and park). We aimed to validate the trails’ design by assessing the physiological response to unsupervised walking trails of: (1) different intensities in COPD patients, and (2) same intensity from different public spaces in healthy adults. Methods On different days and under standardized conditions, 10 COPD patients walked the three intensity trails designed in a boulevard space, and 10 healthy subjects walked the three intensity trails in three different spaces. We measured physiological response and energy expenditure using a gas analyzer. We compared outcomes across trails intensity and/or spaces using mixed-effects linear regression. Results In COPD patients, physiological response and energy expenditure increased significantly according to the trails intensity: mean (SD) peak V˙O2 15.9 (3.5), 17.4 (4.7), and 17.7 (4.4) mL/min/kg (p-trend = 0.02), and MET-min 60 (23), 64 (26), 72 (31) (p-trend<0.01) in low, moderate and high intensity trails, respectively. In healthy subjects there were no differences in physiological response to walking trails of the same intensity across different spaces. Conclusions We validated the trails design for the training of COPD patients by showing that the physiological response to and energy expenditure on unsupervised walking these trails increased according to the predefined trails’ intensity and did not change across trails of the same intensity in different public space. Walkable public spaces allow the design of trails that could be used for the training of COPD patients in the community. PMID:26766184

  18. Effect of trailing edge shape on the wake and propulsive performance of pitching panels

    NASA Astrophysics Data System (ADS)

    van Buren, Tyler; Floryan, Daniel; Brunner, Daniel; Senturk, Utku; Smits, Alexander

    2016-11-01

    We present the effects of the trailing edge shape on the wake and propulsive performance of a pitching panel with an aspect ratio of 1. The trailing edges are symmetric chevron shapes with convex and concave orientations of varying degree. Concave trailing edges delay the natural vortex bending and compression of the wake, and the streamwise velocity field contains a single jet-like structure. Conversely, convex trailing edges promote wake compression and produce a wake split into four jets. Deviation from the square trailing edge mostly reduces the thrust and efficiency. Supported by the Office of Naval Research under MURI Grant Number N00014-14-1-0533.

  19. Design and implementation of an audit trail in compliance with US regulations.

    PubMed

    Jiang, Keyuan; Cao, Xiang

    2011-10-01

    Audit trails have been used widely to ensure quality of study data and have been implemented in computerized clinical trials data systems. Increasingly, there is a need to audit access to study participant identifiable information to provide assurance that study participant privacy is protected and confidentiality is maintained. In the United States, several federal regulations specify how the audit trail function should be implemented. To describe the development and implementation of a comprehensive audit trail system that meets the regulatory requirements of assuring data quality and integrity and protecting participant privacy and that is also easy to implement and maintain. The audit trail system was designed and developed after we examined regulatory requirements, data access methods, prevailing application architecture, and good security practices. Our comprehensive audit trail system was developed and implemented at the database level using a commercially available database management software product. It captures both data access and data changes with the correct user identifier. Documentation of access is initiated automatically in response to either data retrieval or data change at the database level. Currently, our system has been implemented only on one commercial database management system. Although our audit trail algorithm does not allow for logging aggregate operations, aggregation does not reveal sensitive private participant information. Careful consideration must be given to data items selected for monitoring because selection of all data items using our system can dramatically increase the requirements for computer disk space. Evaluating the criticality and sensitivity of individual data items selected can control the storage requirements for clinical trial audit trail records. Our audit trail system is capable of logging data access and data change operations to satisfy regulatory requirements. Our approach is applicable to virtually any data

  20. Chemical cues used by prairie rattlesnakes (Crotalus viridis) to follow trails of rodent prey.

    PubMed

    Chiszar, D; Melcer, T; Lee, R; Radcliffe, C W; Duvall, D

    1990-01-01

    Each of 10 prairie rattlesnakes (Crotalus viridis) was exposed to three types of trails after striking rodent prey (Mus musculus). One trail was made with mouse urine, another was made with tap water, and the third consisted of materials from mouse integument. The snakes exhibited trailing behavior only when integumentary trails were available. It was concluded that prairie rattlesnakes do not utilize urinary cues; instead they attend to materials associated with rodent skin and fur.

  1. 36 CFR 261.12 - National Forest System roads and trails.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... by a sign. (c) Damaging and leaving in a damaged condition any such road, trail, or segment thereof... 36 Parks, Forests, and Public Property 2 2014-07-01 2014-07-01 false National Forest System roads... AGRICULTURE PROHIBITIONS General Prohibitions § 261.12 National Forest System roads and trails. The following...

  2. 36 CFR 261.12 - National Forest System roads and trails.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... by a sign. (c) Damaging and leaving in a damaged condition any such road, trail, or segment thereof... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false National Forest System roads... AGRICULTURE PROHIBITIONS General Prohibitions § 261.12 National Forest System roads and trails. The following...

  3. 36 CFR 261.12 - National Forest System roads and trails.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... by a sign. (c) Damaging and leaving in a damaged condition any such road, trail, or segment thereof... 36 Parks, Forests, and Public Property 2 2013-07-01 2013-07-01 false National Forest System roads... AGRICULTURE PROHIBITIONS General Prohibitions § 261.12 National Forest System roads and trails. The following...

  4. 36 CFR 261.12 - National Forest System roads and trails.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... by a sign. (c) Damaging and leaving in a damaged condition any such road, trail, or segment thereof... 36 Parks, Forests, and Public Property 2 2012-07-01 2012-07-01 false National Forest System roads... AGRICULTURE PROHIBITIONS General Prohibitions § 261.12 National Forest System roads and trails. The following...

  5. 36 CFR 261.12 - National Forest System roads and trails.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... by a sign. (c) Damaging and leaving in a damaged condition any such road, trail, or segment thereof... 36 Parks, Forests, and Public Property 2 2011-07-01 2011-07-01 false National Forest System roads... AGRICULTURE PROHIBITIONS General Prohibitions § 261.12 National Forest System roads and trails. The following...

  6. Multiple effects of TRAIL in human carcinoma cells: Induction of apoptosis, senescence, proliferation, and cytokine production

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Levina, Vera; Marrangoni, Adele M.; DeMarco, Richard

    TRAIL is a death ligand that induces apoptosis in malignant but not normal cells. Recently the ability of TRAIL to induce proliferation in apoptosis-resistant normal and malignant cells was reported. In this study, we analyzed TRAIL effects in apoptosis sensitive MCF7, OVCAR3 and H460 human tumor cell lines. TRAIL at low concentrations preferentially induced cell proliferation. At 100 ng/ml, apoptotic death was readily observed, however surviving cells acquired higher proliferative capacity. TRAIL-stimulated production of several cytokines, IL-8, RANTES, MCP-1 and bFGF, and activation of caspases 1 and 8 was essential for this effect. Antibodies to IL-8, RANTES, and bFGF blockedmore » TRAIL-induced cell proliferation and further stimulated apoptosis. For the first time, we report that high TRAIL concentrations induced cell senescence as determined by the altered morphology and expression of several senescence markers: SA-{beta}-gal, p21{sup Waf1/Cip1}, p16{sup INK4a}, and HMGA. Caspase 9 inhibition protected TRAIL-treated cells from senescence, whereas inhibition of caspases 1 and 8 increased the yield of SLP cells. In conclusion, in cultured human carcinoma cells, TRAIL therapy results in three functional outcomes, apoptosis, proliferation and senescence. TRAIL-induced proapoptotic and prosurvival responses correlate with the strength of signaling. TRAIL-induced cytokine production is responsible for its proliferative and prosurvival effects.« less

  7. Evaluation of Algorithms for a Miles-in-Trail Decision Support Tool

    NASA Technical Reports Server (NTRS)

    Bloem, Michael; Hattaway, David; Bambos, Nicholas

    2012-01-01

    Four machine learning algorithms were prototyped and evaluated for use in a proposed decision support tool that would assist air traffic managers as they set Miles-in-Trail restrictions. The tool would display probabilities that each possible Miles-in-Trail value should be used in a given situation. The algorithms were evaluated with an expected Miles-in-Trail cost that assumes traffic managers set restrictions based on the tool-suggested probabilities. Basic Support Vector Machine, random forest, and decision tree algorithms were evaluated, as was a softmax regression algorithm that was modified to explicitly reduce the expected Miles-in-Trail cost. The algorithms were evaluated with data from the summer of 2011 for air traffic flows bound to the Newark Liberty International Airport (EWR) over the ARD, PENNS, and SHAFF fixes. The algorithms were provided with 18 input features that describe the weather at EWR, the runway configuration at EWR, the scheduled traffic demand at EWR and the fixes, and other traffic management initiatives in place at EWR. Features describing other traffic management initiatives at EWR and the weather at EWR achieved relatively high information gain scores, indicating that they are the most useful for estimating Miles-in-Trail. In spite of a high variance or over-fitting problem, the decision tree algorithm achieved the lowest expected Miles-in-Trail costs when the algorithms were evaluated using 10-fold cross validation with the summer 2011 data for these air traffic flows.

  8. KRISTINA: Kinematic rib-based structural system for innovative adaptive trailing edge

    NASA Astrophysics Data System (ADS)

    Pecora, R.; Amoroso, F.; Magnifico, M.; Dimino, I.; Concilio, A.

    2016-04-01

    Nature teaches that the flight of the birds succeeds perfectly since they are able to change the shape of their wings in a continuous manner. The careful observation of this phenomenon has re-introduced in the recent research topics the study of "metamorphic" wing structures; these innovative architectures allow for the controlled wing shape adaptation to different flight conditions with the ultimate goal of getting desirable improvements such as the increase of aerodynamic efficiency or load control effectiveness. In this framework, the European research project SARISTU aimed at combining morphing and smart ideas to the leading edge, the trailing edge and the winglet of a large commercial airplane (EASA CS25 category) while assessing integrated technologies validation through high-speed wind tunnel test on a true scale outer wing segment. The design process of the adaptive trailing edge (ATED) addressed by SARISTU is here outlined, from the conceptual definition of the camber-morphing architecture up to the assessment of the device executive layout. Rational design criteria were implemented in order to preliminarily define ATED structural layout and the general configuration of the embedded mechanisms enabling morphing under the action of aerodynamic loads. Advanced FE analyses were then carried out and the robustness of adopted structural arrangements was proven in compliance with applicable airworthiness requirements.

  9. The impacts of trail infrastructure on vegetation and soils: Current literature and future directions.

    PubMed

    Ballantyne, Mark; Pickering, Catherine Marina

    2015-12-01

    Reflecting the popularity of nature-based activities such as hiking and mountain biking, there are thousands of kilometres of recreational trails worldwide traversing a range of natural areas. These trails have environmental impacts on soils and vegetation, but where has there been research, what impacts have been found and how were they measured? Using a systematic quantitative literature review methodology, we assessed the impacts of trails on vegetation and soils, highlighting what is known, but also key knowledge gaps. Of the 59 original research papers identified on this topic that have been published in English language peer-reviewed academic journals, most were for research conducted in protected areas (71%), with few from developing countries (17%) or threatened ecosystems (14%). The research is concentrated in a few habitats and biodiversity hotspots, mainly temperate woodland, alpine grassland and Mediterranean habitats, often in the USA (32%) or Australia (20%). Most examined formal trails, with just 15% examining informal trails and 11% assessing both types. Nearly all papers report the results of observational surveys (90%), collecting quantitative data (66%) with 24% using geographic information systems. There was an emphasis on assessing trail impacts at a local scale, either on the trail itself and/or over short gradients away from the trail edge. Many assessed changes in composition and to some degree, structure, of vegetation and soils with the most common impacts documented including reduced vegetation cover, changes in plant species composition, trail widening, soil loss and soil compaction. There were 14 papers assessing how these local impacts can accumulate at the landscape scale. Few papers assessed differences in impacts among trails (7 papers), changes in impacts over time (4), species-specific responses (3) and only one assessed effects on plant community functioning. This review provides evidence that there are key research gaps

  10. Discussion on "The Trail" from the Perspective of Christianism Theology

    ERIC Educational Resources Information Center

    Wang, Jing

    2008-01-01

    Kafka is a writer of strong religious complex. In "The Trail," he illustrates his religious thoughts by probing into the alienation of modern human beings from the God and also shows his pursuit and befuddlement of beliefs. This paper analyzes the crimes and punishment in "The Trail" through three parts, the accusation of…

  11. 75 FR 77897 - Long Walk National Historic Trail Feasibility Study, Abbreviated Final Environmental Impact...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-14

    ... DEPARTMENT OF THE INTERIOR National Park Service [7700-1104-SZS] Long Walk National Historic Trail Feasibility Study, Abbreviated Final Environmental Impact Statement, National Trails Intermountain Region, NM... Environmental Impact Statement for the Long Walk National Historic Trail Feasibility Study. SUMMARY: Pursuant to...

  12. Comprehensive Trail Making Test Performance in Children and Adolescents with Traumatic Brain Injury

    ERIC Educational Resources Information Center

    Allen, Daniel N.; Thaler, Nicholas S.; Ringdahl, Erik N.; Barney, Sally J.; Mayfield, Joan

    2012-01-01

    The sensitivity of the Trail Making Test to brain damage has been well-established over many years, making it one of the most commonly used tests in clinical neuropsychological evaluations. The current study examined the validity of scores from a newer version of the Trail Making Test, the Comprehensive Trail Making Test (CTMT), in children and…

  13. Research for the development of best management practices for minimizing horse trail impacts on the Hoosier National Forest

    USGS Publications Warehouse

    Aust, M.W.; Marion, J.L.; Kyle, K.

    2005-01-01

    This research investigates horse trail impacts to gain an improved understanding of the relationship between various levels of horse use, horse trail management alternatives, and subsequent horse trail degradation. A survey of existing horse trails on the Hoosier National Forest was used to collect data on use-related, environmental and management factors to model horse trail impacts. Results are analyzed to identify which factors are most easily manipulated by managers to effectively avoid and minimize horse trail impacts. A specific focus includes evaluating the relative effect of trail use level, surfacing, grade, and water control on indices of erosion and trafficability such as trail cross sectional area, estimated erosion, muddiness, and incision. Overall, the Hoosier National Forest horse trails could be significantly improved by relocating or closing inherited trails that directly ascend slope or are excessively steep, reducing the distance between water control structures, and by applying gravel to harden trail surfaces and reduce soil erosion. A set of Best Management Practices for trails are included as a product of this work, with recommendations based on this research.

  14. Scope and frequency of fluconazole trailing assessed using EUCAST in invasive Candida spp. isolates.

    PubMed

    Marcos-Zambrano, Laura Judith; Escribano, Pilar; Sánchez-Carrillo, Carlos; Bouza, Emilio; Guinea, Jesús

    2016-10-01

    Trailing is a well-known phenomenon that is defined as reduced but persistent visible growth of Candida spp. at fluconazole concentrations above the MIC. Trailing is commonly detected using the CLSI M27-A3 method, although little is known about its frequency when investigated with EUCAST. We assessed the frequency and scope of fluconazole trailing after using EUCAST EDef 7.2. against a large number of Candida spp. isolates from patients with candidemia. We studied 639 fluconazole-susceptible non-krusei Candida spp. isolates from 570 patients admitted to Gregorio Marañón Hospital. Isolates were tested in vitro for fluconazole susceptibility according to the EUCAST EDef 7.2 procedure; trailing was defined as the presence of any residual growth in wells containing fluconazole concentrations above the MIC. According to the mean percentage of trailing observed, isolates were classified as residual trailers (0.1-5%), slight trailers (6%-10%), moderate trailers (11%-15%), and heavy trailers (>15%). The relationship between trailing and genotyping was assessed. The mean overall percentage of trailing was 6.8%, with C. albicans and C. tropicalis showing the highest percentages (9.75% and 9.29%, respectively; P < .001). C. albicans and C. tropicalis had the highest percentage of heavy trailers (>15%). Trailing was not genotype-specific. Fluconazole trailing was observed frequently when EUCAST was used for antifungal susceptibility testing, particularly in isolates of C. albicans and C. tropicalis The cut-off proposed enabled us to classify the isolates according to the degree of trailing and can be used as the basis for future studies to evaluate the clinical impact of this phenomenon. © The Author 2016. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  15. Reusable Reinforcement Learning via Shallow Trails.

    PubMed

    Yu, Yang; Chen, Shi-Yong; Da, Qing; Zhou, Zhi-Hua

    2018-06-01

    Reinforcement learning has shown great success in helping learning agents accomplish tasks autonomously from environment interactions. Meanwhile in many real-world applications, an agent needs to accomplish not only a fixed task but also a range of tasks. For this goal, an agent can learn a metapolicy over a set of training tasks that are drawn from an underlying distribution. By maximizing the total reward summed over all the training tasks, the metapolicy can then be reused in accomplishing test tasks from the same distribution. However, in practice, we face two major obstacles to train and reuse metapolicies well. First, how to identify tasks that are unrelated or even opposite with each other, in order to avoid their mutual interference in the training. Second, how to characterize task features, according to which a metapolicy can be reused. In this paper, we propose the MetA-Policy LEarning (MAPLE) approach that overcomes the two difficulties by introducing the shallow trail. It probes a task by running a roughly trained policy. Using the rewards of the shallow trail, MAPLE automatically groups similar tasks. Moreover, when the task parameters are unknown, the rewards of the shallow trail also serve as task features. Empirical studies on several controlling tasks verify that MAPLE can train metapolicies well and receives high reward on test tasks.

  16. A volatile trail Pheromone of the Leaf-Cutting Ant, Atta Texana

    Treesearch

    J. H. Tumlinson; John C. Moser; R. M. Silverstein; R. G. Brownlee; J. M. Ruth

    1972-01-01

    The major volatile trail-marking pheromone of the Texas leaf-cutting Ant, Atta texana, was isolated, identified as methyl 4-methylpyrrole-2-carboxylate, and synthesized. The synthesized pheromone elicited strong trail-following response from workers in the laboratory and field.

  17. Nonadecadienone, a new termite trail-following pheromone identified in Glossotermes oculatus (Serritermitidae).

    PubMed

    Hanus, Robert; Šobotník, Jan; Krasulová, Jana; Jiroš, Pavel; Žáček, Petr; Kalinová, Blanka; Dolejšová, Klára; Cvačka, Josef; Bourguignon, Thomas; Roisin, Yves; Lacey, Michael J; Sillam-Dussès, David

    2012-01-01

    Within the multitude of chemical signals used by termites, the trail marking by means of pheromones is ubiquitous. Chemistry and biology of the trail-following communication have been described in more than 60 species from all families except for the Neotropical Serritermitidae. The chemical ecology of Serritermitidae is of special interest not only as a missing piece of knowledge on the diversity and evolution of isopteran pheromones but also because it may contribute to the debate on the phylogenetic position of this family, which is still unresolved. Therefore, we aimed in this study to identify the trail-following pheromone of the serritermitid Glossotermes oculatus. Based on a combined approach of analytical chemistry, electrophysiology, and behavioral bioassays, we propose (10Z,13Z)-nonadeca-10,13-dien-2-one to be the trail-following pheromone of G. oculatus, secreted by the sternal gland of pseudergates. Thus, we report on a new termite trail-following pheromone of an unexpected chemical structure, a ketone with 19 carbons, contrasting with unsaturated alcohols containing 12 carbons as trail-following pheromones in other advanced termite families. In addition to this unique trail-following pheromone, we also describe the sternal gland in pseudergates as an organ of unusual shape, size, and structure when compared with other isopteran species. These results underline the peculiarity of the family Serritermitidae and prompt our interest in the chemistry of pheromones in the other genus of the family, Serritermes. © The Author 2011. Published by Oxford University Press. All rights reserved.

  18. Ship trail/cloud dynamic effects from Apollo-Soyuz photograph July 16, 1975

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Porch, W.M.; Kao, Chih-yue J.; Kyle, T.G.

    1988-01-01

    We describe in this paper the results of a preliminary analysis of a ship trail photograph taken by the Apollo-Soyuz crew at 22:21 GMT on 16 July 1975. The photograph was taken from an altitude of 174 km and shows three separate ship trails with two of the trails intersecting. Because these photographs were taken from a non-geosynchronous satellite with a high-resolution camera, the quality of the photograph provides more detail than is usually obtained from meteorological satellites (minimum spatial resolution 14 m compared to 57 m from Landsat). The photograph not only shows enhanced detail of the ship trailsmore » themselves, but also cloud free bands generated by the ship trails. The ship trails have maximum photographed widths of 3--6 km. These cloud free bands are an obvious indication of the importance of ship trail cloud dynamics to ship trial development. These cloud dynamical effects are driven both by the initial energy release of the ship's power plant and by latent heat release from the aerosol nucleation process. Since the aerosol nucleation process is the key to understanding ocean aerosol/cloud interactions, it is important to partition these two processes in the ship trial development. We will describe in this paper preliminary numerical modeling efforts to simulate the ship trails using only the energy release from the ship and thereby give an indication of how much more energy input may be required from the nucleation process. 12 refs., 6 figs.« less

  19. Monitoring Appalachian Trail corridors: an example of volunteer land management

    Treesearch

    Robert S. Bristow; Greg Knoettner; Rick Wagner

    1998-01-01

    The management relationship between the Appalachian Trail Conference (ATC) and various land management agencies such as the National Park Service (NPS) is a prime example of the Partnerships prescribed by the President's Commission on Americans Outdoors. The Appalachian Trail is one success story of bringing public and private resources together to help plan and...

  20. The novel thymidylate synthase inhibitor trifluorothymidine (TFT) and TRAIL synergistically eradicate non-small cell lung cancer cells.

    PubMed

    Azijli, Kaamar; van Roosmalen, Ingrid A M; Smit, Jorn; Pillai, Saravanan; Fukushima, Masakazu; de Jong, Steven; Peters, Godefridus J; Bijnsdorp, Irene V; Kruyt, Frank A E

    2014-06-01

    TRAIL, a tumor selective anticancer agent, may be used for the treatment of non-small cell lung cancer (NSCLC). However, TRAIL resistance is frequently encountered. Here, the combined use of TRAIL with trifluorothymidine (TFT), a thymidylate synthase inhibitor, was examined for sensitizing NSCLC cells to TRAIL. Interactions between TRAIL and TFT were studied in NSCLC cells using growth inhibition and apoptosis assays. Western blotting and flow cytometry were used to investigate underlying mechanisms. The combined treatment of TFT and TRAIL showed synergistic cytotoxicity in A549, H292, H322 and H460 cells. For synergistic activity, the sequence of administration was important; TFT treatment followed by TRAIL exposure did not show sensitization. Combined TFT and TRAIL treatment for 24 h followed by 48 h of TFT alone was synergistic in all cell lines, with combination index values below 0.9. The treatments affected cell cycle progression, with TRAIL inducing a G1 arrest and TFT, a G2/M arrest. TFT activated Chk2 and reduced Cdc25c levels known to cause G2/M arrest. TRAIL-induced caspase-dependent apoptosis was enhanced by TFT, whereas TFT alone mainly induced caspase-independent death. TFT increased the expression of p53 and p21/WAF1, and p53 was involved in the increase of TRAIL-R2 surface expression. TFT also caused downregulation of cFLIP and XIAP and increased Bax expression. TFT enhances TRAIL-induced apoptosis in NSCLC cells by sensitizing the apoptotic machinery at different levels in the TRAIL pathway. Our findings suggest a possible therapeutic benefit of the combined use of TFT and TRAIL in NSCLC.

  1. TRAIL-induced programmed necrosis as a novel approach to eliminate tumor cells

    PubMed Central

    2014-01-01

    Background The cytokine TRAIL represents one of the most promising candidates for the apoptotic elimination of tumor cells, either alone or in combination therapies. However, its efficacy is often limited by intrinsic or acquired resistance of tumor cells to apoptosis. Programmed necrosis is an alternative, molecularly distinct mode of programmed cell death that is elicited by TRAIL under conditions when the classical apoptosis machinery fails or is actively inhibited. The potential of TRAIL-induced programmed necrosis in tumor therapy is, however, almost completely uncharacterized. We therefore investigated its impact on a panel of tumor cell lines of wide-ranging origin. Methods Cell death/viability was measured by flow cytometry/determination of intracellular ATP levels/crystal violet staining. Cell surface expression of TRAIL receptors was detected by flow cytometry, expression of proteins by Western blot. Ceramide levels were quantified by high-performance thin layer chromatography and densitometric analysis, clonogenic survival of cells was determined by crystal violet staining or by soft agarose cloning. Results TRAIL-induced programmed necrosis killed eight out of 14 tumor cell lines. Clonogenic survival was reduced in all sensitive and even one resistant cell lines tested. TRAIL synergized with chemotherapeutics in killing tumor cell lines by programmed necrosis, enhancing their effect in eight out of 10 tested tumor cell lines and in 41 out of 80 chemotherapeutic/TRAIL combinations. Susceptibility/resistance of the investigated tumor cell lines to programmed necrosis seems to primarily depend on expression of the pro-necrotic kinase RIPK3 rather than the related kinase RIPK1 or cell surface expression of TRAIL receptors. Furthermore, interference with production of the lipid ceramide protected all tested tumor cell lines. Conclusions Our study provides evidence that TRAIL-induced programmed necrosis represents a feasible approach for the elimination of

  2. A New Serial-direction Trail Effect in CCD Images of the Lunar-based Ultraviolet Telescope

    NASA Astrophysics Data System (ADS)

    Wu, C.; Deng, J. S.; Guyonnet, A.; Antilogus, P.; Cao, L.; Cai, H. B.; Meng, X. M.; Han, X. H.; Qiu, Y. L.; Wang, J.; Wang, S.; Wei, J. Y.; Xin, L. P.; Li, G. W.

    2016-10-01

    Unexpected trails have been seen subsequent to relative bright sources in astronomical images taken with the CCD camera of the Lunar-based Ultraviolet Telescope (LUT) since its first light on the Moon’s surface. The trails can only be found in the serial-direction of CCD readout, differing themselves from image trails of radiation-damaged space-borne CCDs, which usually appear in the parallel-readout direction. After analyzing the same trail defects following warm pixels (WPs) in dark frames, we found that the relative intensity profile of the LUT CCD trails can be expressed as an exponential function of the distance i (in number of pixels) of the trailing pixel to the original source (or WP), i.e., {\\mathtt{\\exp }}(α {\\mathtt{i}}+β ). The parameters α and β seem to be independent of the CCD temperature, intensity of the source (or WP), and its position in the CCD frame. The main trail characteristics show evolution occurring at an increase rate of ˜(7.3 ± 3.6) × 10-4 in the first two operation years. The trails affect the consistency of the profiles of different brightness sources, which make smaller aperture photometry have larger extra systematic error. The astrometric uncertainty caused by the trails is too small to be acceptable based on LUT requirements for astrometry accuracy. Based on the empirical profile model, a correction method has been developed for LUT images that works well for restoring the fluxes of astronomical sources that are lost in trailing pixels.

  3. Influence of Cattle Trails on Runoff Quantity and Quality.

    PubMed

    Miller, Jim J; Curtis, Tony; Chanasyk, David S; Willms, Walter D

    2017-03-01

    Cattle trails in grazed pastures close to rivers may adversely affect surface water quality of the adjacent river by directing runoff to it. The objective of this 3-yr study (2013-2015) in southern Alberta, Canada, was to determine if cattle trails significantly increased the risk of runoff and contaminants (sediment, nutrients) compared with the adjacent grazed pasture (control). A portable rainfall simulator was used to generate artificial rainfall (140 mm h) and runoff. The runoff properties measured were time to runoff and initial abstraction (infiltration), total runoff depth and average runoff rates, as well as concentrations and mass loads of sediment, N, and P fractions. Cattle trails significantly ( ≤ 0.10) decreased time to runoff and initial abstraction (26-32%) in the 2 yr measured and increased total runoff depth, runoff coefficients, and average runoff rates (21-51%) in 2 of 3 yr. Concentrations of sediment, N, and P fractions in runoff were not significantly greater for cattle trails than for control areas. However, mass loads of total suspended solids (57-85% increase), NH-N (31-90%), and dissolved reactive P (DRP) (30-92%) were significantly greater because of increased runoff volumes. Overall, runoff quantity and loads of sediment, NH-N, and DRP were greater for cattle trails compared with the adjacent grazed pasture, and hydrologic connection with cattle-access sites on the riverbank suggests that this could adversely affect water quality in the adjacent river. Extrapolation of the study results should be tempered by the specific conditions represented by this rainfall simulation study. Copyright © by the American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America, Inc.

  4. Effects of the Built Environment on Childhood Obesity: the Case of Urban Recreational Trails and Crime

    PubMed Central

    Sandy, Robert; Tchernis, Rusty; Wilson, Jeff; Liu, Gilbert; Zhou, Xilin

    2012-01-01

    We study the effects of urban environment on childhood obesity by concentrating on the effects of walking trails and crime close to children’s homes on their BMI and obesity status. We use a unique dataset, which combines information on recreational trails in Indianapolis with data on violent crimes and anthropomorphic and diagnostic data from children’s clinic visits between 1996 and 2005. We find that having a trail near a home reduces children’s weight. However, the effect depends on the amount of nearby violent crimes. Significant reductions occur only in low crime areas and trails could have opposite effects on weight in high crime areas. These effects are primarily among boys, older children, and children who live in higher income neighborhoods. Evaluated at the mean length of trails this effect for older children in no crime areas would be a reduction of two pounds of the body weight. Falsification tests using planned trails instead of existing trails, show that trails are more likely to be located in areas with heavier children, suggesting that our results on effects of trails represent a lower bound. PMID:22459489

  5. Effects of the built environment on childhood obesity: the case of urban recreational trails and crime.

    PubMed

    Sandy, Robert; Tchernis, Rusty; Wilson, Jeffrey; Liu, Gilbert; Zhou, Xilin

    2013-01-01

    We study the effects of urban environment on childhood obesity by concentrating on the effects of walking trails and crime close to children's homes on their BMI and obesity status. We use a unique dataset, which combines information on recreational trails in Indianapolis with data on violent crimes and anthropomorphic and diagnostic data from children's clinic visits between 1996 and 2005. We find that having a trail near a home reduces children's weight. However, the effect depends on the amount of nearby violent crimes. Significant reductions occur only in low crime areas and trails could have opposite effects on weight in high crime areas. These effects are primarily among boys, older children, and children who live in higher income neighborhoods. Evaluated at the mean length of trails this effect for older children in no crime areas would be a reduction of 2 lb of the body weight. Falsification tests using planned trails instead of existing trails, show that trails are more likely to be located in areas with heavier children, suggesting that our results on effects of trails represent a lower bound. Published by Elsevier B.V.

  6. Research to guide trail management at Acadia National Park, Maine

    Treesearch

    Kelly Goonan; Robert Manning; William Valliere

    2009-01-01

    Acadia National Park, Maine, is the tenth most-visited national park in the United States. Managers face the challenge of protecting the park's trail system from damage while maintaining a high quality recreation experience. For this study, an initial phase of research was conducted to identify potential indicators of quality for trail resources and the visitor...

  7. Skipping on uneven ground: trailing leg adjustments simplify control and enhance robustness.

    PubMed

    Müller, Roy; Andrada, Emanuel

    2018-01-01

    It is known that humans intentionally choose skipping in special situations, e.g. when descending stairs or when moving in environments with lower gravity than on Earth. Although those situations involve uneven locomotion, the dynamics of human skipping on uneven ground have not yet been addressed. To find the reasons that may motivate this gait, we combined experimental data on humans with numerical simulations on a bipedal spring-loaded inverted pendulum model (BSLIP). To drive the model, the following parameters were estimated from nine subjects skipping across a single drop in ground level: leg lengths at touchdown, leg stiffness of both legs, aperture angle between legs, trailing leg angle at touchdown (leg landing first after flight phase), and trailing leg retraction speed. We found that leg adjustments in humans occur mostly in the trailing leg (low to moderate leg retraction during swing phase, reduced trailing leg stiffness, and flatter trailing leg angle at lowered touchdown). When transferring these leg adjustments to the BSLIP model, the capacity of the model to cope with sudden-drop perturbations increased.

  8. A locally-blazed ant trail achieves efficient collective navigation despite limited information

    PubMed Central

    Fonio, Ehud; Heyman, Yael; Boczkowski, Lucas; Gelblum, Aviram; Kosowski, Adrian; Korman, Amos; Feinerman, Ofer

    2016-01-01

    Any organism faces sensory and cognitive limitations which may result in maladaptive decisions. Such limitations are prominent in the context of groups where the relevant information at the individual level may not coincide with collective requirements. Here, we study the navigational decisions exhibited by Paratrechina longicornis ants as they cooperatively transport a large food item. These decisions hinge on the perception of individuals which often restricts them from providing the group with reliable directional information. We find that, to achieve efficient navigation despite partial and even misleading information, these ants employ a locally-blazed trail. This trail significantly deviates from the classical notion of an ant trail: First, instead of systematically marking the full path, ants mark short segments originating at the load. Second, the carrying team constantly loses the guiding trail. We experimentally and theoretically show that the locally-blazed trail optimally and robustly exploits useful knowledge while avoiding the pitfalls of misleading information. DOI: http://dx.doi.org/10.7554/eLife.20185.001 PMID:27815944

  9. Use Deflected Trailing Edge to Improve the Aerodynamic Performance and Develop Low Solidity LPT Cascade

    NASA Astrophysics Data System (ADS)

    Chao, Li; Peigang, Yan; Xiangfeng, Wang; Wanjin, Han; Qingchao, Wang

    2017-08-01

    This paper investigates the feasibility of improving the aerodynamic performance of low pressure turbine (LPT) blade cascades and developing low solidity LPT blade cascades through deflected trailing edge. A deflected trailing edge improved aerodynamic performance of both LPT blade cascades and low solidity LPT blade cascades. For standard solidity LPT cascades, deflecting the trailing edge can decrease the energy loss coefficient by 20.61 % for a Reynolds number (Re) of 25,000 and freestream turbulence intensities (FSTI) of 1 %. For a low solidity LPT cascade, aerodynamic performance was also improved by deflecting the trailing edge. Solidity of the LPT cascade can be reduced by 12.5 % for blades with a deflected trailing edge without a drop in efficiency. Here, the flow control mechanism surrounding a deflected trailing edge was also revealed.

  10. Life on the Oregon Trail.

    ERIC Educational Resources Information Center

    Middle Level Learning: Teaching and Learning Social Studies in the Middle Grades, 1998

    1998-01-01

    This supplement to "Social Education" and "Social Studies & the Young Learner" seeks to support creative and rigorous social studies teaching in middle schools. The articles show how students can revisit the Oregon Trail through the diaries of children, learn about the five themes of geography (location, place,…

  11. Expression of osteoprotegerin and its ligands, RANKL and TRAIL, in rheumatoid arthritis

    PubMed Central

    Remuzgo-Martínez, Sara; Genre, Fernanda; López-Mejías, Raquel; Ubilla, Begoña; Mijares, Verónica; Pina, Trinitario; Corrales, Alfonso; Blanco, Ricardo; Martín, Javier; Llorca, Javier; González-Gay, Miguel A.

    2016-01-01

    Osteoprotegerin (OPG), receptor activator of nuclear factor-ΚB ligand (RANKL) and tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) have been involved in rheumatoid arthritis (RA) pathophysiology. In this study, we assessed messenger RNA (mRNA) expression of these molecules by qPCR in peripheral blood from 26 patients with RA (12 of them with ischemic heart disease –IHD) and 10 healthy controls. Correlation coefficients between OPG, RANKL and TRAIL expression levels in RA patients and their clinical and demographic characteristics were also evaluated. Whereas OPG and OPG/TRAIL ratio expression were significantly increased in RA patients compared to controls (fold change = 1.79, p = 0.013 and 2.07, p = 0.030, respectively), RANKL/OPG ratio was significantly decreased (fold change = 0.50, p = 0.020). No significant differences were found between patients and controls in RANKL and TRAIL expression. Interestingly, TRAIL expression was significantly higher in RA patients with IHD compared to those without IHD (fold change = 1.46, p = 0.033). Moreover, biologic disease-modifying antirheumatic drugs (DMARDs) significantly decreased RANKL expression in RA patients (p = 0.016). Our study supports an important role of OPG and TRAIL in RA. Furthermore, it highlights an effect of biologic DMARDs in the modulation of RANKL. PMID:27403809

  12. Trails of the leafcutters

    Treesearch

    John C. Moser

    1967-01-01

    The trails of leaf-cutting ants are among the most conspicuous and long-lived of all ant roadways. In tropical America, where such ants are abundant, paths leading from underground nests are often a foot wide and extended for 100 yards or more to trees or other plants whose leaves the ants gather. The ants commonly carry their forage above their heads, and when the...

  13. Absence of death receptor translocation into lipid rafts in acquired TRAIL-resistant NSCLC cells.

    PubMed

    Ouyang, Wen; Yang, Chunxu; Zhang, Simin; Liu, Yu; Yang, Bo; Zhang, Junhong; Zhou, Fuxiang; Zhou, Yunfeng; Xie, Conghua

    2013-02-01

    Resistance to tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a major limitation for its clinical use. The mechanisms of TRAIL resistance have been mostly studied in the context of cell lines that are intrinsically resistant to TRAIL. However, little is known about the molecular alterations that contribute to the development of acquired resistance during treatment with TRAIL. In this study, we established H460R, an isogenic cell line with acquired TRAIL resistance, from the TRAIL‑sensitive human lung cancer cell line H460 to investigate the mechanisms of acquired resistance. The acquired TRAIL‑resistant H460R cells remained sensitive to cisplatin. The mRNA and protein expression levels of death receptor 4 (DR4) and death receptor 5 (DR5) were not altered in either of the TRAIL-treated cell lines. Nevertheless, tests in which the DR4 or DR5 gene was overexpressed or silenced suggest that death receptor expression is necessary but not sufficient for TRAIL‑induced apoptosis. Compared with parental TRAIL-sensitive H460 cells, H460R cells showed a decreased TRAIL-induced translocation of DR4/DR5 into lipid rafts. Further studies showed that nystatin partially prevented lipid raft aggregation and DR4 and DR5 clustering and reduced apoptosis in H460 cells again. Analysis of apoptotic molecules showed that more pro-caspase-8, FADD, caspase-3 and Bid, but less cFLIP in H460 cells than in H460R cells. Our findings suggest that the lack of death receptor redistribution negatively impacts DISC assembly in lipid rafts, which at least partially leads to the development of acquired resistance to TRAIL in H460R cells.

  14. Breaking the cipher: ant eavesdropping on the variational trail pheromone of its termite prey

    PubMed Central

    Wen, Xiao-Lan

    2017-01-01

    Predators may eavesdrop on their prey using innate signals of varying nature. In regards to social prey, most of the prey signals are derived from social communication and may therefore be highly complex. The most efficient predators select signals that provide the highest benefits. Here, we showed the use of eusocial prey signals by the termite-raiding ant Odontoponera transversa. O. transversa selected the trail pheromone of termites as kairomone in several species of fungus-growing termites (Termitidae: Macrotermitinae: Odontotermes yunnanensis, Macrotermes yunnanensis, Ancistrotermes dimorphus). The most commonly predated termite, O. yunnanensis, was able to regulate the trail pheromone component ratios during its foraging activity. The ratio of the two trail pheromone compounds was correlated with the number of termites in the foraging party. (3Z)-Dodec-3-en-1-ol (DOE) was the dominant trail pheromone component in the initial foraging stages when fewer termites were present. Once a trail was established, (3Z,6Z)-dodeca-3,6-dien-1-ol (DDE) became the major recruitment component in the trail pheromone and enabled mass recruitment of nest-mates to the food source. Although the ants could perceive both components, they revealed stronger behavioural responses to the recruitment component, DDE, than to the common major component, DOE. In other words, the ants use the trail pheromone information as an indication of suitable prey abundance, and regulate their behavioural responses based on the changing trail pheromone component. The eavesdropping behaviour in ants therefore leads to an arms race between predator and prey where the species specific production of trail pheromones in termites is targeted by predatory ant species. PMID:28446695

  15. Breaking the cipher: ant eavesdropping on the variational trail pheromone of its termite prey.

    PubMed

    Wen, Xiao-Lan; Wen, Ping; Dahlsjö, Cecilia A L; Sillam-Dussès, David; Šobotník, Jan

    2017-04-26

    Predators may eavesdrop on their prey using innate signals of varying nature. In regards to social prey, most of the prey signals are derived from social communication and may therefore be highly complex. The most efficient predators select signals that provide the highest benefits. Here, we showed the use of eusocial prey signals by the termite-raiding ant Odontoponera transversa O. transversa selected the trail pheromone of termites as kairomone in several species of fungus-growing termites (Termitidae: Macrotermitinae: Odontotermes yunnanensis , Macrotermes yunnanensis , Ancistrotermes dimorphus ). The most commonly predated termite, O. yunnanensis, was able to regulate the trail pheromone component ratios during its foraging activity. The ratio of the two trail pheromone compounds was correlated with the number of termites in the foraging party. (3 Z )-Dodec-3-en-1-ol (DOE) was the dominant trail pheromone component in the initial foraging stages when fewer termites were present. Once a trail was established, (3 Z,6Z )-dodeca-3,6-dien-1-ol (DDE) became the major recruitment component in the trail pheromone and enabled mass recruitment of nest-mates to the food source. Although the ants could perceive both components, they revealed stronger behavioural responses to the recruitment component, DDE, than to the common major component, DOE. In other words, the ants use the trail pheromone information as an indication of suitable prey abundance, and regulate their behavioural responses based on the changing trail pheromone component. The eavesdropping behaviour in ants therefore leads to an arms race between predator and prey where the species specific production of trail pheromones in termites is targeted by predatory ant species. © 2017 The Author(s).

  16. Trail-following pheromones in basal termites, with special reference to Mastotermes darwiniensis.

    PubMed

    Sillam-Dussès, David; Sémon, Etienne; Lacey, Michael J; Robert, Alain; Lenz, Michael; Bordereau, Christian

    2007-10-01

    In the framework of an evolutionary study, trail pheromones have been studied in the most basal extant termite, Mastotermes darwiniensis (Mastotermitidae), and two other basal termites, the Termopsidae Porotermes adamsoni (Porotermitinae) and Stolotermes victoriensis (Stolotermitinae). Although workers of M. darwiniensis do not walk in single file while exploring a new environment under experimental conditions and are unable to follow artificial trails in 'open field' experiments, they do secrete a trail-following pheromone from their sternal glands. This unique behavior might reflect a primitive function of communication of the sternal gland. The major component of the pheromone appears to be the same in the three basal species: the norsesquiterpene alcohol (E)-2,6,10-trimethyl-5,9-undecadien-1-ol. This represents a new chemical category of trail-following pheromones for termites. The quantity of pheromone was estimated as 20 pg/individual in M. darwiniensis, 700 pg/individual in P. adamsoni, and 4 pg/individual in S. victoriensis. The activity threshold was 1 ng/cm in M. darwiniensis and 10 pg/cm in P. adamsoni. In M. darwiniensis, the trail pheromone was secreted by sternal gland 4 and to a lesser degree by sternal gland 3, sternal gland 5 being almost inactive. This study highlighted phylogenetic relationships between the Mastotermitidae and two subfamilies of the Termopsidae, the Porotermitinae and the Stolotermitinae. Furthermore, it indicated a heterogeneity within the Termopsidae, with Porotermitinae and Stolotermitinae on one hand, and Termopsinae on the other. Finally, Mastotermitidae and Termopsidae, with C14 trail pheromones, are clearly separated from the Kalotermitidae, Rhinotermitidae, and Termitidae that secrete C12 or C20 trail pheromones.

  17. The influence of sampling interval on the accuracy of trail impact assessment

    USGS Publications Warehouse

    Leung, Y.-F.; Marion, J.L.

    1999-01-01

    Trail impact assessment and monitoring (IA&M) programs have been growing in importance and application in recreation resource management at protected areas. Census-based and sampling-based approaches have been developed in such programs, with systematic point sampling being the most common survey design. This paper examines the influence of sampling interval on the accuracy of estimates for selected trail impact problems. A complete census of four impact types on 70 trails in Great Smoky Mountains National Park was utilized as the base data set for the analyses. The census data were resampled at increasing intervals to create a series of simulated point data sets. Estimates of frequency of occurrence and lineal extent for the four impact types were compared with the census data set. The responses of accuracy loss on lineal extent estimates to increasing sampling intervals varied across different impact types, while the responses on frequency of occurrence estimates were consistent, approximating an inverse asymptotic curve. These findings suggest that systematic point sampling may be an appropriate method for estimating the lineal extent but not the frequency of trail impacts. Sample intervals of less than 100 m appear to yield an excellent level of accuracy for the four impact types evaluated. Multiple regression analysis results suggest that appropriate sampling intervals are more likely to be determined by the type of impact in question rather than the length of trail. The census-based trail survey and the resampling-simulation method developed in this study can be a valuable first step in establishing long-term trail IA&M programs, in which an optimal sampling interval range with acceptable accuracy is determined before investing efforts in data collection.

  18. Elastically Deformable Side-Edge Link for Trailing-Edge Flap Aeroacoustic Noise Reduction

    NASA Technical Reports Server (NTRS)

    Khorrami, Mehdi R. (Inventor); Lockard, David P. (Inventor); Moore, James B. (Inventor); Su, Ji (Inventor); Turner, Travis L. (Inventor); Lin, John C. (Inventor); Taminger, Karen M. (Inventor); Kahng, Seun K. (Inventor); Verden, Scott A. (Inventor)

    2014-01-01

    A system is provided for reducing aeroacoustic noise generated by an aircraft having wings equipped with trailing-edge flaps. The system includes a plurality of elastically deformable structures. Each structure is coupled to and along one of the side edges of one of the trailing-edge flaps, and is coupled to a portion of one of the wings that is adjacent to the one of the side edges. The structures elastically deform when the trailing-edge flaps are deployed away from the wings.

  19. Facile one-pot formulation of TRAIL-embedded paclitaxel-bound albumin nanoparticles for the treatment of pancreatic cancer.

    PubMed

    Min, Sun Young; Byeon, Hyeong Jun; Lee, Changkyu; Seo, Jisoo; Lee, Eun Seong; Shin, Beom Soo; Choi, Han-Gon; Lee, Kang Choon; Youn, Yu Seok

    2015-10-15

    Nanoparticle albumin-bound (nab™) technology is an effective way of delivering hydrophobic chemotherapeutics. We developed a one-pot/one-step formulation of paclitaxel (PTX)-bound albumin nanoparticles with embedded tumor necrosis factor-related apoptosis-inducing ligand (TRAIL/PTX HSA-NP) for the treatment of pancreatic cancer. TRAIL/PTX HSA-NPs were fabricated using a high-pressure homogenizer at a TRAIL feeding ratio of 0.2%, 1.0%, and 2.0%. TRAIL/PTX HSA-NPs were spherical and became larger in size (170-230 nm) with increasing TRAIL amount (0.2-2.0%). The loading efficiencies of PTX were in the range of ∼86.4% and significantly low at 2.0% TRAIL (60.4%). Specifically, the inhibitory concentrations (IC50) of TRAIL (1.0 or 2.0%)/PTX HSA-NPs were >20-fold lower than that of plain PTX-HSA NP (0.032±0.06, 0.022±0.005, and 0.96±0.15 ng/ml, respectively) in pancreatic Mia Paca-2 cells. Considering TRAIL loading, bioactivity, and particle size, TRAIL(1.0%)/PTX HSA-NPs were determined as the optimal candidate for further studies. TRAIL(1.0%)/PTX HSA-NPs displayed substantially greater apoptotic activity than plain PTX HSA-NP in both FACS and TUNEL analysis. The loaded PTX and TRAIL were gradually released from the TRAIL(1.0%)/PTX HSA-NPs until ∼24 h, which is considered to be a sufficient time for delivery to the tumor tissue. TRAIL(1.0%)/PTX HSA-NP displayed markedly more antitumor efficacy than plain PTX HSA-NP in Mia Paca-2 cell-xenografted mice in terms of tumor volume (size) and weight (213.9 mm(3) and 0.18 g vs. 1126.8 mm(3) and 0.80 g, respectively). These improved in vitro and in vivo performances were due to the combined synergistic effects of PTX and TRAIL. We believe that this TRAIL/PTX HSA-NP would have potential as a novel apoptosis-based anticancer agent. Copyright © 2015 Elsevier B.V. All rights reserved.

  20. 75 FR 27771 - Overland Trail Transmission, LLC; Notice of Baseline Filing

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-18

    ... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. PR10-23-000] Overland Trail Transmission, LLC; Notice of Baseline Filing May 11, 2010. Take notice that on May 7, 2010, Overland Trail Transmission, LLC submitted its baseline filing of its Statement of Operating Conditions for transportation...

  1. Fascaplysin sensitizes cells to TRAIL-induced apoptosis through upregulating DR5 expression

    NASA Astrophysics Data System (ADS)

    Wang, Feng; Chen, Haimin; Yan, Xiaojun; Zheng, Yanling

    2013-05-01

    This study investigated the molecular mechanism of anti-tumor effect of fascaplysin, a nitrogenous red pigment firstly isolated from a marine sponge. Microarray analysis show that the TNF and TNF receptor superfamily in human umbilical vein endothelial cells (HUVEC) and human hepatocarcinoma cells (BEL-7402) were significantly regulated by fascaplysin. Western Blot results reveal that fascaplysin increased the expression of cleaved caspase-9, active caspase-3, and decreased the level of procaspase-8 and Bid. Flow cytometry and cytotoxicity tests indicate that fascaplysin sensitized cells to tumor necrosis-related apoptosisinducing ligand-(TRAIL) induced apoptosis, which was markedly blocked by TRAIL R2/Fc chimera, a dominant negative form of TRAIL receptor DR5. Therefore, our results demonstrate that fascaplysin promotes apoptosis through the activation of TRAIL signaling pathway by upregulating DR5 expression.

  2. Identification of trail pheromone of larva of eastern tent caterpillarMalacosoma americanum (Lepidoptera: Lasiocampidae).

    PubMed

    Crump, D; Silverstein, R M; Williams, H J; Fitzgerald, T D

    1987-03-01

    Previous studies have shown that larvae of the eastern tent caterpillar (Malacosoma americanum F.) mark trails, leading from their tent to feeding sites on host trees, with a pheromone secreted from the posterior tip of the abdominal sternum. 5β-Cholestane-3,24-dione (1) has been identified as an active component of the trail. The larvae have a threshold sensitivity to the pheromone of 10(-11) g/mm of trail. Several related compounds elicit the trail-following response. Two other species of tent caterpillars also responded positively to the pheromone in preliminary laboratory tests.

  3. Use and users of the Appalachian Trail: a geographic study

    Treesearch

    Robert E. Manning; William Valliere; Jim Bacon; Alan Graefe; Gerard Kyle; Rita Hennessy

    2001-01-01

    The Appalachian National Scenic Trail (AT) is a public footpath that spans 2,160 miles of Appalachian Mountain ridgelines from Maine to Georgia. This paper describes the first comprehensive study of recreational use and users of the AT. The primary study method was a survey of visitors to the AT. The Trail was divided into 22 relatively homogeneous sections within four...

  4. Audit Trail Management System in Community Health Care Information Network.

    PubMed

    Nakamura, Naoki; Nakayama, Masaharu; Nakaya, Jun; Tominaga, Teiji; Suganuma, Takuo; Shiratori, Norio

    2015-01-01

    After the Great East Japan Earthquake we constructed a community health care information network system. Focusing on the authentication server and portal server capable of SAML&ID-WSF, we proposed an audit trail management system to look over audit events in a comprehensive manner. Through implementation and experimentation, we verified the effectiveness of our proposed audit trail management system.

  5. State Secret: North Carolina and the Cherokee Trail of Tears

    ERIC Educational Resources Information Center

    Bryant, James

    2008-01-01

    This paper is an analytic essay that examines the treatment of the Cherokee Trail of Tears in a North Carolina fourth grade textbook. I begin by offering a satiric look at an imaginary textbook's treatment of the Holocaust that is based closely on the actual narrative of the Trail of Tears written in the fourth grade text. Following this, close…

  6. Spatially characterizing visitor use and its association with informal trails in Yosemite Valley meadows.

    PubMed

    Walden-Schreiner, Chelsey; Leung, Yu-Fai

    2013-07-01

    Ecological impacts associated with nature-based recreation and tourism can compromise park and protected area goals if left unrestricted. Protected area agencies are increasingly incorporating indicator-based management frameworks into their management plans to address visitor impacts. Development of indicators requires empirical evaluation of indicator measures and examining their ecological and social relevance. This study addresses the development of the informal trail indicator in Yosemite National Park by spatially characterizing visitor use in open landscapes and integrating use patterns with informal trail condition data to examine their spatial association. Informal trail and visitor use data were collected concurrently during July and August of 2011 in three, high-use meadows of Yosemite Valley. Visitor use was clustered at statistically significant levels in all three study meadows. Spatial data integration found no statistically significant differences between use patterns and trail condition class. However, statistically significant differences were found between the distance visitors were observed from informal trails and visitor activity type with active activities occurring closer to trail corridors. Gender was also found to be significant with male visitors observed further from trail corridors. Results highlight the utility of integrated spatial analysis in supporting indicator-based monitoring and informing management of open landscapes. Additional variables for future analysis and methodological improvements are discussed.

  7. Spatially Characterizing Visitor Use and Its Association with Informal Trails in Yosemite Valley Meadows

    NASA Astrophysics Data System (ADS)

    Walden-Schreiner, Chelsey; Leung, Yu-Fai

    2013-07-01

    Ecological impacts associated with nature-based recreation and tourism can compromise park and protected area goals if left unrestricted. Protected area agencies are increasingly incorporating indicator-based management frameworks into their management plans to address visitor impacts. Development of indicators requires empirical evaluation of indicator measures and examining their ecological and social relevance. This study addresses the development of the informal trail indicator in Yosemite National Park by spatially characterizing visitor use in open landscapes and integrating use patterns with informal trail condition data to examine their spatial association. Informal trail and visitor use data were collected concurrently during July and August of 2011 in three, high-use meadows of Yosemite Valley. Visitor use was clustered at statistically significant levels in all three study meadows. Spatial data integration found no statistically significant differences between use patterns and trail condition class. However, statistically significant differences were found between the distance visitors were observed from informal trails and visitor activity type with active activities occurring closer to trail corridors. Gender was also found to be significant with male visitors observed further from trail corridors. Results highlight the utility of integrated spatial analysis in supporting indicator-based monitoring and informing management of open landscapes. Additional variables for future analysis and methodological improvements are discussed.

  8. The effect of TRAIL molecule on cell viability in in vitro beta cell culture.

    PubMed

    Tekmen, I; Ozyurt, D; Pekçetin, C; Buldan, Z

    2007-06-01

    Insulin-dependent diabetes mellitus (IDDM) is an organ-specific autoimmune disorder triggered by autoreactive T cells directed to pancreas beta-cell antigens. In this disorder, more than 90% of beta cells are destroyed. Cell death may be mediated via soluble or membrane-bound cell death ligands. One of these ligands may be tumour necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL), a member of the TNF-alpha superfamily. In the present study, we examined whether TRAIL had cytotoxic effects on adult rat pancreas beta cell cultures and INS1-E rat insulinoma cell line cultures or not. In this study, cell destruction models were built with TRAIL concentrations of 10, 100 and 1000 ng. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test was used for evaluating cell viability. It was detected that cell cultures with TRAIL added showed no differences statistically when compared with control cultures containing no toxic additions. These results showed that TRAIL did not have significant cytotoxic effects on pancreas beta cell culture and INS-1E rat insulinoma cell line cultures. Detection of the expression of TRAIL receptors and natural apoptosis inhibitor proteins will be favourable to investigate the resistance mechanisms to TRAIL-induced cell death in this cell culture system.

  9. Water in the trail of the Chelyabinsk bolide

    NASA Astrophysics Data System (ADS)

    Gladysheva, O. G.

    2017-09-01

    At 03:20 UTC on February 15, 2013 a very bright bolide entered Earth's atmosphere. Fragments of the meteorite fell to the earth's surface. Examination of these fragments revealed that several of them were located directly on the surface of the celestial body [1], while the majority lay at a depth of less than 2.5 m from the surface [2, 3]. The stone meteorite's durability, >15 MPa, corresponded to <1% of the initial mass, while the rest of the object possessed a low durability of 1 MPa [4]. Moreover, Fe3+ hydroxyls were discovered in meteorite samples, the formation of which required water [5]. The glow at the head of the bolide trail, lasting 8 seconds after the flight of the object, and the development of the cloud trail indicate that the celestial body carried water. The Chinese weather satellite Feng-Yun 2D discovered ice debris (water) in the bolide trail [6]. Here, we will demonstrate that the Chelyabinsk chondrite was delivered to the Earth by an ice-bearing celestial body.

  10. 75 FR 66124 - Notice of Public Meeting, National Historic Oregon Trail Interpretive Center Advisory Board

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-27

    ...] Notice of Public Meeting, National Historic Oregon Trail Interpretive Center Advisory Board AGENCY: Bureau of Land Management, Interior. ACTION: Meeting Notice for the National Historic Oregon Trail...) National Historic Oregon Trail Interpretive Center (NHOTIC) Advisory Board will meet as indicated below...

  11. Evaluation of the Cross Alert system on the Virginia Capital Trail in James City County.

    DOT National Transportation Integrated Search

    2008-01-01

    The Virginia Capital Trail is a bicycle and pedestrian trail that connects Virginia's past and present capitals of Jamestown, Williamsburg, and Richmond along the scenic Route 5 highway corridor. The trail crosses Route 5, a two-lane roadway with a 5...

  12. The impact of glide phases on the trackability of hydrodynamic trails in harbour seals (Phoca vitulina).

    PubMed

    Wieskotten, S; Dehnhardt, G; Mauck, B; Miersch, L; Hanke, W

    2010-11-01

    The mystacial vibrissae of harbour seals (Phoca vitulina) constitute a highly sensitive hydrodynamic receptor system enabling the seals to detect and follow hydrodynamic trails. In the wild, hydrodynamic trails, as generated by swimming fish, consist of cyclic burst-and-glide phases, associated with various differences in the physical parameters of the trail. Here, we investigated the impact of glide phases on the trackability of differently aged hydrodynamic trails in a harbour seal. As fish are not easily trained to swim certain paths with predetermined burst-and-glide phases, the respective hydrodynamic trails were generated using a remote-controlled miniature submarine. Gliding phases in hydrodynamic trails had a negative impact on the trackability when trails were 15 s old. The seal lost the generated trails more often within the transition zones, when the submarine switched from a burst to a glide moving pattern. Hydrodynamic parameter analysis (particle image velocimetry) revealed that the smaller dimensions and faster decay of hydrodynamic trails generated by the gliding submarine are responsible for the impaired success of the seal tracking the gliding phase. Furthermore, the change of gross water flow generated by the submarine from a rearwards-directed stream in the burst phase to a water flow passively dragged behind the submarine during gliding might influence the ability of the seal to follow the trail as this might cause a weaker deflection of the vibrissae. The possible ecological implications of intermittent swimming behaviour in fish for piscivorous predators are discussed.

  13. Comparative efficacy of multimodal digital methods in assessing trail/resource degradation

    Treesearch

    Logan O. Park

    2014-01-01

    Outdoor recreation can cause both positive and negative impacts on associated forest ecosystems. Forest recreation trails localize negative impacts to a controlled spatial extent while providing recreation access beyond developed areas and transportation networks. Current methods for assessing extent and severity of trail and proximal resource degradation require...

  14. Sex and Age Differences in Trail Half Marathon Running.

    PubMed

    Navalta, James W; Montes, Jeffrey; Tanner, Elizabeth A; Bodell, Nathaniel G; Young, John C

    2018-01-01

    Female participation is growing in trail running races. The purpose was to evaluate sex and age differences in top finishers of a trail running half marathon. Velocity differences between males (M) and females (F) were determined for the top 10 finishers of the Moab Trail Half Marathon from 2012 - 2015 across age, and by finishing place. Differences between age category and between sexes were determined through ANOVA with significance accepted at P < 0.05. A significant difference for running velocity was present between sexes at each age category (20-29 yr F = 2.9±0.3, M = 3.4±0.4 m·sec -1 ; 30-39 yr F = 2.8±0.3, M = 3.3±0.3; 40-49 yr F = 2.7±0.3, M = 3.0±0.5; 50-59 yr F = 2.3±0.2, M = 2.8±0.3; 60-69 yr F = 1.6±0.3, M = 2.2±0.4; P < 0.0001). Sex difference in trail running velocity was consistent (~13%) among all age categories with exception of the oldest group (33%, P = 0.0001). There were significantly greater female finishers in every age category (20 - 29 yr F = 107±18, M = 56±1;, 30 - 39 yr F = 150±34, M = 84±21; 40 - 49 yr F = 112±17, M = 64±16; P < 0.01) until 50 - 59 yr (F = 48±13, M = 41±14; P = 0.50). These data indicate that the widening gap in sex differences observed in road races are ameliorated in a trail running environment that has a larger number of female participants.

  15. On Entropy Trail

    NASA Astrophysics Data System (ADS)

    Farokhi, Saeed; Taghavi, Ray; Keshmiri, Shawn

    2015-11-01

    Stealth technology is developed for military aircraft to minimize their signatures. The primary attention was focused on radar signature, followed by the thermal and noise signatures of the vehicle. For radar evasion, advanced configuration designs, extensive use of carbon composites and radar-absorbing material, are developed. On thermal signature, mainly in the infra-red (IR) bandwidth, the solution was found in blended rectangular nozzles of high aspect ratio that are shielded from ground detectors. For noise, quiet and calm jets are integrated into vehicles with low-turbulence configuration design. However, these technologies are totally incapable of detecting new generation of revolutionary aircraft. These shall use all electric, distributed, propulsion system that are thermally transparent. In addition, composite skin and non-emitting sensors onboard the aircraft will lead to low signature. However, based on the second-law of thermodynamics, there is no air vehicle that can escape from leaving an entropy trail. Entropy is thus the only inevitable signature of any system, that once measured, can detect the source. By characterizing the entropy field based on its statistical properties, the source may be recognized, akin to face recognition technology. Direct measurement of entropy is cumbersome, however as a derived property, it can be easily measured. The measurement accuracy depends on the probe design and the sensors onboard. One novel air data sensor suite is introduced with promising potential to capture the entropy trail.

  16. Wallula Basalt Pilot Demonstration Project: Post-injection Results and Conclusions

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    McGrail, Bernard Pete; Schaef, Herbert T.; Spane, Frank A.

    Deep underground geologic formations are emerging as a reasonable option for long-term storage of CO 2, including large continental flood basalt formations. At the GHGT-11 and GHGT-12 conferences, progress was reported on the initial phases for Wallula Basalt Pilot demonstration test (located in Eastern Washington state), where nearly 1,000 metric tons of CO 2 were injected over a 3-week period during July/August 2013. The target CO 2 injection intervals were two permeable basalt interflow reservoir zones with a combined thickness of ~20 m that occur within a layered basalt sequence between a depth of 830-890 m below ground surface. Duringmore » the two-year post-injection period, downhole fluid samples were periodically collected during this post-injection monitoring phase, coupled with limited wireline borehole logging surveys that provided indirect evidence of on-going chemical geochemical reactions/alterations and CO 2 disposition. A final detailed post-closure field characterization program that included downhole fluid sampling, and performance of hydrologic tests and wireline geophysical surveys. Included as part of the final wireline characterization activities was the retrieval of side-wall cores from within the targeted injection zones. These cores were examined for evidence of in-situ mineral carbonization. Visual observations of the core material identified small globular nodules, translucent to yellow in color, residing within vugs and small cavities of the recovered basalt side-wall cores, which were not evident in pre-injection side-wall cores obtained from the native basalt formation. Characterization by x-ray diffraction identified these nodular precipitates as ankerite, a commonly occurring iron and calcium rich carbonate. Isotopic characterization (δ 13C, δ 18O) conducted on the ankerite nodules indicate a distinct isotopic signature that is closely aligned with that of the injected CO 2. Both the secondary mineral nodules and injected CO 2

  17. Wallula Basalt Pilot Demonstration Project: Post-injection Results and Conclusions

    DOE PAGES

    McGrail, Bernard Pete; Schaef, Herbert T.; Spane, Frank A.; ...

    2017-08-18

    Deep underground geologic formations are emerging as a reasonable option for long-term storage of CO 2, including large continental flood basalt formations. At the GHGT-11 and GHGT-12 conferences, progress was reported on the initial phases for Wallula Basalt Pilot demonstration test (located in Eastern Washington state), where nearly 1,000 metric tons of CO 2 were injected over a 3-week period during July/August 2013. The target CO 2 injection intervals were two permeable basalt interflow reservoir zones with a combined thickness of ~20 m that occur within a layered basalt sequence between a depth of 830-890 m below ground surface. Duringmore » the two-year post-injection period, downhole fluid samples were periodically collected during this post-injection monitoring phase, coupled with limited wireline borehole logging surveys that provided indirect evidence of on-going chemical geochemical reactions/alterations and CO 2 disposition. A final detailed post-closure field characterization program that included downhole fluid sampling, and performance of hydrologic tests and wireline geophysical surveys. Included as part of the final wireline characterization activities was the retrieval of side-wall cores from within the targeted injection zones. These cores were examined for evidence of in-situ mineral carbonization. Visual observations of the core material identified small globular nodules, translucent to yellow in color, residing within vugs and small cavities of the recovered basalt side-wall cores, which were not evident in pre-injection side-wall cores obtained from the native basalt formation. Characterization by x-ray diffraction identified these nodular precipitates as ankerite, a commonly occurring iron and calcium rich carbonate. Isotopic characterization (δ 13C, δ 18O) conducted on the ankerite nodules indicate a distinct isotopic signature that is closely aligned with that of the injected CO 2. Both the secondary mineral nodules and injected CO 2

  18. Large eddy simulation of trailing edge noise

    NASA Astrophysics Data System (ADS)

    Keller, Jacob; Nitzkorski, Zane; Mahesh, Krishnan

    2015-11-01

    Noise generation is an important engineering constraint to many marine vehicles. A significant portion of the noise comes from propellers and rotors, specifically due to flow interactions at the trailing edge. Large eddy simulation is used to investigate the noise produced by a turbulent 45 degree beveled trailing edge and a NACA 0012 airfoil. A porous surface Ffowcs-Williams and Hawkings acoustic analogy is combined with a dynamic endcapping method to compute the sound. This methodology allows for the impact of incident flow noise versus the total noise to be assessed. LES results for the 45 degree beveled trailing edge are compared to experiment at M = 0 . 1 and Rec = 1 . 9 e 6 . The effect of boundary layer thickness on sound production is investigated by computing using both the experimental boundary layer thickness and a thinner boundary layer. Direct numerical simulation results of the NACA 0012 are compared to available data at M = 0 . 4 and Rec = 5 . 0 e 4 for both the hydrodynamic field and the acoustic field. Sound intensities and directivities are investigated and compared. Finally, some of the physical mechanisms of far-field noise generation, common to the two configurations, are discussed. Supported by Office of Naval research.

  19. Westward Expansion: The Oregon Trail.

    ERIC Educational Resources Information Center

    Salisbury, James F.

    This 8-week interdisciplinary unit for fourth- and fifth-grade students helps children address the U.S. westward expansion in the 1840's using the interactive software program, The Oregon Trail. The unit provides connections to literature, geography, computer/mathematics skills, language arts, and research skills. The work is done in cooperative…

  20. Describing Trails: Distance or Time?

    ERIC Educational Resources Information Center

    Morris, Beverly R.; Bixler, Robert D.

    1998-01-01

    To determine whether trails on visitor maps should indicate distance only or include "time to complete," Cleveland Metroparks interviewed 287 adult visitors to a large regional zoo. The mean perceived "average time to walk a mile" was 17 minutes, but responses ranged from 1.5 to 60 minutes. Half of respondents underestimated…

  1. Carving a New Assessment Trail

    ERIC Educational Resources Information Center

    Morriston, Terry

    2007-01-01

    TRAILS (Tool for Real-Time Assessment of Information Literacy Skills), is a free online test of student information-handling skills. It was formulated by the Institute for Library and Information Literacy Education and Kent State University Libraries. Based on the Ohio Academic Content Standards and the philosophy of Information Power, it assesses…

  2. Impacts of informal trails on vegetation and soils in the highest protected area in the Southern Hemisphere.

    PubMed

    Barros, Agustina; Gonnet, Jorge; Pickering, Catherine

    2013-09-30

    There is limited recreation ecology research in South America, especially studies looking at informal trails. Impacts of informal trails formed by hikers and pack animals on vegetation and soils were assessed for the highest protected area in the Southern Hemisphere, Aconcagua Provincial Park. The number of braided trails, their width and depth were surveyed at 30 sites along the main access route to Mt Aconcagua (6962 m a.s.l.). Species composition, richness and cover were also measured on control and trail transects. A total of 3.3 ha of alpine meadows and 13.4 ha of alpine steppe were disturbed by trails. Trails through meadows resulted in greater soil loss, more exposed soil and rock and less vegetation than trails through steppe vegetation. Trampling also affected the composition of meadow and steppe vegetation with declines in sedges, herbs, grasses and shrubs on trails. These results highlight how visitor use can result in substantial cumulative damage to areas of high conservation value in the Andes. With unregulated use of trails and increasing visitation, park agencies need to limit the further spread of informal trails and improve the conservation of plant communities in Aconcagua Provincial Park and other popular parks in the region. Copyright © 2013 Elsevier Ltd. All rights reserved.

  3. Developing a monitoring protocol for visitor-created informal trails in Yosemite National Park, USA.

    PubMed

    Leung, Yu-Fai; Newburger, Todd; Jones, Marci; Kuhn, Bill; Woiderski, Brittany

    2011-01-01

    Informal trails created or perpetuated by visitors is a management challenge in many protected natural areas such as Yosemite National Park. This is a significant issue as informal trail networks penetrate and proliferate into protected landscapes and habitats, threatening ecological integrity, aesthetics, and visitor experiences. In order to develop effective strategies for addressing this problem under an adaptive management framework, indicators must be developed and monitoring protocol must be established to gather timely and relevant data about the condition, extent, and distribution of these undesired trail segments. This article illustrates a process of developing and evaluating informal trail indicators for meadows in Yosemite Valley. Indicator measures developed in past research were reviewed to identify their appropriateness for the current application. Information gaps in existing indicator measures were addressed by creating two new indices to quantify the degree of informal trailing based on its land fragmentation effects. The selected indicator measures were applied to monitoring data collected between 2006 and 2008. The selected measures and indices were evaluated for their ability to characterize informal trail impacts at site and landscape scales. Results demonstrate the utility of indicator measures in capturing different characteristics of the informal trail problem, though several metrics are strongly related to each other. The two fragmentation indices were able to depict fragmentation without being too sensitive to changes in one constituent parameter. This study points to the need for a multiparameter approach to informal trail monitoring and integration with other monitoring data. Implications for monitoring programs and research are discussed.

  4. Developing a Monitoring Protocol for Visitor-Created Informal Trails in Yosemite National Park, USA

    NASA Astrophysics Data System (ADS)

    Leung, Yu-Fai; Newburger, Todd; Jones, Marci; Kuhn, Bill; Woiderski, Brittany

    2011-01-01

    Informal trails created or perpetuated by visitors is a management challenge in many protected natural areas such as Yosemite National Park. This is a significant issue as informal trail networks penetrate and proliferate into protected landscapes and habitats, threatening ecological integrity, aesthetics, and visitor experiences. In order to develop effective strategies for addressing this problem under an adaptive management framework, indicators must be developed and monitoring protocol must be established to gather timely and relevant data about the condition, extent, and distribution of these undesired trail segments. This article illustrates a process of developing and evaluating informal trail indicators for meadows in Yosemite Valley. Indicator measures developed in past research were reviewed to identify their appropriateness for the current application. Information gaps in existing indicator measures were addressed by creating two new indices to quantify the degree of informal trailing based on its land fragmentation effects. The selected indicator measures were applied to monitoring data collected between 2006 and 2008. The selected measures and indices were evaluated for their ability to characterize informal trail impacts at site and landscape scales. Results demonstrate the utility of indicator measures in capturing different characteristics of the informal trail problem, though several metrics are strongly related to each other. The two fragmentation indices were able to depict fragmentation without being too sensitive to changes in one constituent parameter. This study points to the need for a multiparameter approach to informal trail monitoring and integration with other monitoring data. Implications for monitoring programs and research are discussed.

  5. Managing outdoor recreation conflict on the Squamish, British Columbia Trail Network

    Treesearch

    Ana Elia Ramón Hidalgo; Howard Harshaw

    2012-01-01

    Recreationists with high expectations of satisfaction from outdoor recreation activities are increasingly using trails networks near urban areas. But differences in expectations, behaviors and values of trail users may create conflicts resulting in unsatisfactory experiences. The objective of this study was to test the efficacy of management practices that may reduce...

  6. Silencing the epigenetic silencer KDM4A for TRAIL and DR5 simultaneous induction and antitumor therapy.

    PubMed

    Wang, Junjian; Wang, Haibin; Wang, Ling-Yu; Cai, Demin; Duan, Zhijian; Zhang, Yanhong; Chen, Peng; Zou, June X; Xu, Jianzhen; Chen, Xinbin; Kung, Hsing-Jien; Chen, Hong-Wu

    2016-11-01

    Recombinant TRAIL and agonistic antibodies to death receptors (DRs) have been in clinical trial but displayed limited anti-cancer efficacy. Lack of functional DR expression in tumors is a major limiting factor. We report here that chromatin regulator KDM4A/JMJD2A, not KDM4B, has a pivotal role in silencing tumor cell expression of both TRAIL and its receptor DR5. In TRAIL-sensitive and -resistant cancer cells of lung, breast and prostate, KDM4A small-molecule inhibitor compound-4 (C-4) or gene silencing strongly induces TRAIL and DR5 expression, and causes TRAIL-dependent apoptotic cell death. KDM4A inhibition also strongly sensitizes cells to TRAIL. C-4 alone potently inhibits tumor growth with marked induction of TRAIL and DR5 expression in the treated tumors and effectively sensitizes them to the newly developed TRAIL-inducer ONC201. Mechanistically, C-4 does not appear to act through the Akt-ERK-FOXO3a pathway. Instead, it switches histone modifying enzyme complexes at promoters of TRAIL and DR5 transcriptional activator CHOP gene by dissociating KDM4A and nuclear receptor corepressor (NCoR)-HDAC complex and inducing the recruitment of histone acetylase CBP. Thus, our results reveal KDM4A as a key epigenetic silencer of TRAIL and DR5 in tumors and establish inhibitors of KDM4A as a novel strategy for effectively sensitizing tumors to TRAIL pathway-based therapeutics.

  7. Silencing the epigenetic silencer KDM4A for TRAIL and DR5 simultaneous induction and antitumor therapy

    PubMed Central

    Wang, Junjian; Wang, Haibin; Wang, Ling-Yu; Cai, Demin; Duan, Zhijian; Zhang, Yanhong; Chen, Peng; Zou, June X; Xu, Jianzhen; Chen, Xinbin; Kung, Hsing-Jien; Chen, Hong-Wu

    2016-01-01

    Recombinant TRAIL and agonistic antibodies to death receptors (DRs) have been in clinical trial but displayed limited anti-cancer efficacy. Lack of functional DR expression in tumors is a major limiting factor. We report here that chromatin regulator KDM4A/JMJD2A, not KDM4B, has a pivotal role in silencing tumor cell expression of both TRAIL and its receptor DR5. In TRAIL-sensitive and -resistant cancer cells of lung, breast and prostate, KDM4A small-molecule inhibitor compound-4 (C-4) or gene silencing strongly induces TRAIL and DR5 expression, and causes TRAIL-dependent apoptotic cell death. KDM4A inhibition also strongly sensitizes cells to TRAIL. C-4 alone potently inhibits tumor growth with marked induction of TRAIL and DR5 expression in the treated tumors and effectively sensitizes them to the newly developed TRAIL-inducer ONC201. Mechanistically, C-4 does not appear to act through the Akt-ERK-FOXO3a pathway. Instead, it switches histone modifying enzyme complexes at promoters of TRAIL and DR5 transcriptional activator CHOP gene by dissociating KDM4A and nuclear receptor corepressor (NCoR)-HDAC complex and inducing the recruitment of histone acetylase CBP. Thus, our results reveal KDM4A as a key epigenetic silencer of TRAIL and DR5 in tumors and establish inhibitors of KDM4A as a novel strategy for effectively sensitizing tumors to TRAIL pathway-based therapeutics. PMID:27612013

  8. TRAIL Death Receptor-4 Expression Positively Correlates With the Tumor Grade in Breast Cancer Patients With Invasive Ductal Carcinoma

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Sanlioglu, Ahter D.; Department of Medical Biology and Genetics, Akdeniz University Faculty of Medicine, Antalya; Korcum, Aylin F.

    2007-11-01

    Purpose: Tumor necrosis factor-related apoptosis inducing ligand (TRAIL) selectively induces apoptosis in cancer cells but not in normal cells, and a number of clinical trials have recently been initiated to test the safety and antitumoral potential of TRAIL in cancer patients. Four different receptors have been identified to interact with TRAIL: two are death-inducing receptors (TRAIL-R1 [DR4] and TRAIL-R2 [DR5]), whereas the other two (TRAIL-R3 [DcR1] and TRAIL-R4 [DcR2]) do not induce death upon ligation and are believed to counteract TRAIL-induced cytotoxicity. Because high levels of DcR2 expression have recently been correlated with carcinogenesis in the prostate and lung, thismore » study investigated the importance of TRAIL and TRAIL receptor expression in breast cancer patients with invasive ductal carcinoma, taking various prognostic markers into consideration. Methods and Materials: Immunohistochemical analyses were performed on 90 breast cancer patients with invasive ductal carcinoma using TRAIL and TRAIL receptor-specific antibodies. Age, menopausal status, tumor size, lymph node status, tumor grade, lymphovascular invasion, perineural invasion, extracapsular tumor extension, presence of an extensive intraductal component, multicentricity, estrogen and progesterone receptor status, and CerbB2 expression levels were analyzed with respect to TRAIL/TRAIL receptor expression patterns. Results: The highest TRAIL receptor expressed in patients with invasive ductal carcinoma was DR4. Although progesterone receptor-positive patients exhibited lower DR5 expression, CerbB2-positive tissues displayed higher levels of both DR5 and TRAIL expressions. Conclusions: DR4 expression positively correlates with the tumor grade in breast cancer patients with invasive ductal carcinoma.« less

  9. Numerical study of the trailing vortex of a wing with wing-tip blowing

    NASA Technical Reports Server (NTRS)

    Lim, Hock-Bin

    1994-01-01

    Trailing vortices generated by lifting surfaces such as helicopter rotor blades, ship propellers, fixed wings, and canard control surfaces are known to be the source of noise, vibration, cavitation, degradation of performance, and other hazardous problems. Controlling these vortices is, therefore, of practical interest. The formation and behavior of the trailing vortices are studied in the present research. In addition, wing-tip blowing concepts employing axial blowing and spanwise blowing are studied to determine their effectiveness in controlling these vortices and their effects on the performance of the wing. The 3D, unsteady, thin-layer compressible Navier-Stokes equations are solved using a time-accurate, implicit, finite difference scheme that employs LU-ADI factorization. The wing-tip blowing is simulated using the actuator plane concept, thereby, not requiring resolution of the jet slot geometry. Furthermore, the solution blanking feature of the chimera scheme is used to simplify the parametric study procedure for the wing-tip blowing. Computed results are shown to compare favorably with experimental measurements. It is found that axial wing-tip blowing, although delaying the rolling-up of the trailing vortices and the near-field behavior of the flowfield, does not dissipate the circulation strength of the trailing vortex farther downstream. Spanwise wing-tip blowing has the effect of displacing the trailing vortices outboard and upward. The increased 'wing-span' due to the spanwise wing-tip blowing has the effect of lift augmentation on the wing and the strengthening of the trailing vortices. Secondary trailing vortices are created at high spanwise wing-tip blowing intensities.

  10. Achieving success in trail related partnerships: the Michigan State Forest Experience

    Treesearch

    Joel A. Lynch; Charles M. Nelson

    1998-01-01

    Management of trails has become challenging as funding for their operation and administration has decreased. To contend with shortfalls, resources managers are forming partnerships with stakeholders to meet these challenges. An investigation of two trail programs in Michigan is used to illustrate the benefits of partnerships, key elements related to success, and...

  11. Resveratrol Enhances Antitumor Activity of TRAIL in Prostate Cancer Xenografts through Activation of FOXO Transcription Factor

    PubMed Central

    Ganapathy, Suthakar; Chen, Qinghe; Singh, Karan P.; Shankar, Sharmila; Srivastava, Rakesh K.

    2010-01-01

    Background Resveratrol (3, 4′, 5 tri-hydroxystilbene), a naturally occurring polyphenol, exhibits anti-inflammatory, antioxidant, cardioprotective and antitumor activities. We have recently shown that resveratrol can enhance the apoptosis-inducing potential of TRAIL in prostate cancer cells through multiple mechanisms in vitro. Therefore, the present study was designed to validate whether resveratrol can enhance the apoptosis-inducing potential of TRAIL in a xenograft model of prostate cancer. Methodology/Principal Findings Resveratrol and TRAIL alone inhibited growth of PC-3 xenografts in nude mice by inhibiting tumor cell proliferation (PCNA and Ki67 staining) and inducing apoptosis (TUNEL staining). The combination of resveratrol and TRAIL was more effective in inhibiting tumor growth than single agent alone. In xenografted tumors, resveratrol upregulated the expressions of TRAIL-R1/DR4, TRAIL-R2/DR5, Bax and p27/K IP1, and inhibited the expression of Bcl-2 and cyclin D1. Treatment of mice with resveratrol and TRAIL alone inhibited angiogenesis (as demonstrated by reduced number of blood vessels, and VEGF and VEGFR2 positive cells) and markers of metastasis (MMP-2 and MMP-9). The combination of resveratrol with TRAIL further inhibited number of blood vessels in tumors, and circulating endothelial growth factor receptor 2-positive endothelial cells than single agent alone. Furthermore, resveratrol inhibited the cytoplasmic phosphorylation of FKHRL1 resulting in its enhanced activation as demonstrated by increased DNA binding activity. Conclusions/Significance These data suggest that resveratrol can enhance the apoptosis-inducing potential of TRAIL by activating FKHRL1 and its target genes. The ability of resveratrol to inhibit tumor growth, metastasis and angiogenesis, and enhance the therapeutic potential of TRAIL suggests that resveratrol alone or in combination with TRAIL can be used for the management of prostate cancer. PMID:21209944

  12. Comparison of trailside degradation across a gradient of trail use in the Sonoran Desert.

    PubMed

    Rowe, Helen Ivy; Tluczek, Melanie; Broatch, Jennifer; Gruber, Dan; Jones, Steve; Langenfeld, Debbie; McNamara, Peggy; Weinstein, Leona

    2018-02-01

    As recreational visitation to the Sonoran Desert increases, the concern of scientists, managers and advocates who manage its natural resources deepens. Although many studies have been conducted on trampling of undisturbed vegetation and the effects of trails on adjacent plant and soil communities, little such research has been conducted in the arid southwest. We sampled nine 450-m trail segments with different visitation levels in Scottsdale's McDowell Sonoran Preserve over three years to understand the effects of visitation on soil erosion, trailside soil crusts and plant communities. Soil crust was reduced by 27-34% near medium and high use trails (an estimated peak rate of 13-70 visitors per hour) compared with control plots, but there was less than 1% reduction near low use trails (peak rate of two to four visitors per hour). We did not detect soil erosion in the center 80% of the trampled area of any of the trails. The number of perennial plant species dropped by less than one plant species on average, but perennial plant cover decreased by 7.5% in trailside plots compared with control plots 6 m off-trail. At the current levels of visitation, the primary management focus should be keeping people on the originally constructed trail tread surface to reduce impact to adjacent soil crusts. Copyright © 2017 Elsevier Ltd. All rights reserved.

  13. 30 CFR 75.906 - Trailing cables for mobile equipment, ground wires, and ground check wires.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 30 Mineral Resources 1 2013-07-01 2013-07-01 false Trailing cables for mobile equipment, ground... Underground Low- and Medium-Voltage Alternating Current Circuits § 75.906 Trailing cables for mobile equipment, ground wires, and ground check wires. [Statutory Provisions] Trailing cables for mobile equipment shall...

  14. 30 CFR 75.906 - Trailing cables for mobile equipment, ground wires, and ground check wires.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 30 Mineral Resources 1 2012-07-01 2012-07-01 false Trailing cables for mobile equipment, ground... Underground Low- and Medium-Voltage Alternating Current Circuits § 75.906 Trailing cables for mobile equipment, ground wires, and ground check wires. [Statutory Provisions] Trailing cables for mobile equipment shall...

  15. 30 CFR 75.906 - Trailing cables for mobile equipment, ground wires, and ground check wires.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 30 Mineral Resources 1 2014-07-01 2014-07-01 false Trailing cables for mobile equipment, ground... Underground Low- and Medium-Voltage Alternating Current Circuits § 75.906 Trailing cables for mobile equipment, ground wires, and ground check wires. [Statutory Provisions] Trailing cables for mobile equipment shall...

  16. Specificity of laboratory trail following by the argentine ant,Iridomyrmex humilis (Mayr), to (Z)-9-hexadecenal, analogs, and gaster extract.

    PubMed

    Van Vorhis Key, S E; Baker, T C

    1982-07-01

    In laboratory trail-following bioassays of Argentine ant workers,Iridomyrmex humilis (Mayr), the geometric isomer, (E)-9-hexadecenal, of the trail pheromone component (Z)-9-hexadecenal elicited insignificant trail following as did the potentially more stable formate analogs, (Z)-7-tetradecenyl formate, (E)-7-tetradecenyl formate, and tetradecyl formate. Further, in direct choice tests, workers showed no preference for gaster extract trails (0.002 ant equiv/cm) over trails of (Z)-9-hexadecenal (0.2 ng/cm). Moreover, a 10-fold increase in synthetic trail concentration to 2.0 ng/cm caused (Z)-9-hexadecenal trails to be significantly preferred over gaster extract trails by trail-following ants.

  17. ‘Columbia Sunrise’ thornless trailing blackberry

    USDA-ARS?s Scientific Manuscript database

    ‘Columbia Sunrise’ is a new, very early ripening, thornless trailing blackberry (Rubus subg. Rubus Watson) cultivar from the U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS) breeding program in Corvallis, OR, released in cooperation with Oregon State University’s Agricultural ...

  18. A Mathematics and Science Trail

    ERIC Educational Resources Information Center

    Smith, Kathy Horak; Fuentes, Sarah Quebec

    2012-01-01

    In an attempt to engage primary-school students in a hands-on, real-world problem-solving context, a large urban district, a mathematics and science institute housed in a college of education, and a corporate sponsor in the southwest United States, joined forces to create a mathematics and science trail for fourth- and fifth-grade students. A…

  19. Enhancing apoptosis in TRAIL-resistant cancer cells using fundamental response rules

    PubMed Central

    Piras, Vincent; Hayashi, Kentaro; Tomita, Masaru; Selvarajoo, Kumar

    2011-01-01

    The tumor necrosis factor related apoptosis-inducing ligand (TRAIL) induces apoptosis in malignant cells, while leaving other cells mostly unharmed. However, several carcinomas remain resistant to TRAIL. To investigate the resistance mechanisms in TRAIL-stimulated human fibrosarcoma (HT1080) cells, we developed a computational model to analyze the temporal activation profiles of cell survival (IκB, JNK, p38) and apoptotic (caspase-8 and -3) molecules in wildtype and several (FADD, RIP1, TRAF2 and caspase-8) knock-down conditions. Based on perturbation-response approach utilizing the law of information (signaling flux) conservation, we derived response rules for population-level average cell response. From this approach, i) a FADD-independent pathway to activate p38 and JNK, ii) a crosstalk between RIP1 and p38, and iii) a crosstalk between p62 and JNK are predicted. Notably, subsequent simulations suggest that targeting a novel molecule at p62/sequestosome-1 junction will optimize apoptosis through signaling flux redistribution. This study offers a valuable prospective to sensitive TRAIL-based therapy. PMID:22355661

  20. Negative feedback in ants: crowding results in less trail pheromone deposition

    PubMed Central

    Czaczkes, Tomer J.; Grüter, Christoph; Ratnieks, Francis L. W.

    2013-01-01

    Crowding in human transport networks reduces efficiency. Efficiency can be increased by appropriate control mechanisms, which are often imposed externally. Ant colonies also have distribution networks to feeding sites outside the nest and can experience crowding. However, ants do not have external controllers or leaders. Here, we report a self-organized negative feedback mechanism, based on local information, which downregulates the production of recruitment signals in crowded parts of a network by Lasius niger ants. We controlled crowding by manipulating trail width and the number of ants on a trail, and observed a 5.6-fold reduction in the number of ants depositing trail pheromone from least to most crowded conditions. We also simulated crowding by placing glass beads covered in nest-mate cuticular hydrocarbons on the trail. After 10 bead encounters over 20 cm, forager ants were 45 per cent less likely to deposit pheromone. The mechanism of negative feedback reported here is unusual in that it acts by downregulating the production of a positive feedback signal, rather than by direct inhibition or the production of an inhibitory signal. PMID:23365196