Command and data handling of science signals on Spacelab

NASA Technical Reports Server (NTRS)

Mccain, H. G.

1975-01-01

The Orbiter Avionics and the Spacelab Command and Data Management System (CDMS) combine to provide a relatively complete command, control, and data handling service to the instrument complement during a Shuttle Sortie Mission. The Spacelab CDMS services the instruments and the Orbiter in turn services the Spacelab. The CDMS computer system includes three computers, two I/O units, a mass memory, and a variable number of remote acquisition units. Attention is given to the CDMS high rate multiplexer, CDMS tape recorders, closed circuit television for the visual monitoring of payload bay and cabin area activities, methods of science data acquisition, questions of transmission and recording, CDMS experiment computer usage, and experiment electronics.

Vernier Caliper and Micrometer Computer Models Using Easy Java Simulation and Its Pedagogical Design Features--Ideas for Augmenting Learning with Real Instruments

ERIC Educational Resources Information Center

Wee, Loo Kang; Ning, Hwee Tiang

2014-01-01

This paper presents the customization of Easy Java Simulation models, used with actual laboratory instruments, to create active experiential learning for measurements. The laboratory instruments are the vernier caliper and the micrometer. Three computer model design ideas that complement real equipment are discussed. These ideas involve (1) a…

Instrumentation for the Atmospheric Explorer photoelectron spectrometer

NASA Technical Reports Server (NTRS)

Peletier, D. P.

1973-01-01

The photoelectron spectrometer (PES) is part of the complements of scientific instruments aboard three NASA Atmosphere Explorer (AE) satellites. The PES measures the energy spectrum, angular distribution, and intensity of electrons in the earth's thermosphere. Measurements of energies between 2 and 500 eV are made at altitudes as low as 130 km. The design, characteristics, and performance of the instrument are described.

Space Telescope optics. [large aperture astronomical instrument

NASA Technical Reports Server (NTRS)

Jones, C. O.

1979-01-01

The paper reviews the optical technology that has been developed over the last decade for the Space Telescope. The optical design of the telescope, the optical performance control system, and the anticipated optical performance are all presented. Consideration is also given to the initial complement of focal plane instruments.

Spectroscopic observations with the Stratospheric Observatory for Infrared Astronomy (SOFIA)

NASA Astrophysics Data System (ADS)

Becklin, E. E.; Tielens, A. G. G. M.; Callis, H. H. S.

The joint US and German SOFIA project to develop and operate a 2.5-meter infrared airborne telescope in a Boeing 747-SP is now in its final stages of development. Flying in the stratosphere, SOFIA allows observations through the infrared and submillimeter region, with an average transmission of greater than 80%. SOFIA is characterized by a wide instrument complement ranging from broadband imagers, through moderate resolution spectrographs capable of resolving broad features due to dust and large molecules, to high-resolution spectrometers suitable for kinematic studies of molecular and atomic gas lines at km/s resolution. This broad range in instruments will enable SOFIA to make unique contributions to a broad array of science topics. First science flights will begin in 2009 and the observatory is expected to operate for over 20 years. The sensitivity, characteristics, science instrument complement, and examples of first light spectroscopic science are discussed.

Stratospheric Observatory for Infrared Astronomy (sofia)

NASA Astrophysics Data System (ADS)

Becklin, E. E.; Tielens, A. G. G. M.; Callis, H. H. S.

The joint U.S. and German SOFIA project to develop and operate a 2.5-meter infrared airborne telescope in a Boeing 747-SP is now in its final stages of development. Flying in the stratosphere, SOFIA allows observations through the infrared and submillimeter region, with an average transmission of ≳ 80%. SOFIA is characterized by a wide instrument complement ranging from broadband imagers, through moderate resolution spectrographs capable of resolving broad features due to dust and large molecules, to high resolution spectrometers suitable for kinematic studies of molecular and atomic gas lines at km/s resolution. This broad range in instruments will enable SOFIA to make unique contributions to a broad array of science topics. First science flights will begin in 2009 and the observatory is expected to operate for over 20 years. The sensitivity, characteristics, science instrument complement, and examples of first light science are discussed.

The Meteorological Experiment on the Mars Surveyor '98 Polar Lander

NASA Technical Reports Server (NTRS)

Crisp, D.

1999-01-01

When it lands on Mars on December 3, 1999, the Mars Surveyor '98 Mars Polar Lander (MPL) will provide the first opportunity to make in-situ measurements of the near-surface weather climate, and volatile inventory in the Martian south polar region. To make the most of this opportunity, the MPL's Mars Volatiles and Climate Surveyor (MVACS) payload includes the most comprehensive complement of meteorological instruments ever sent to Mars. Like the Viking and the Mars Pathfinder Lander, the MVACS Meteorological (Met) package includes sensors for measuring atmospheric pressures, temperatures, and wind velocities. This payload also includes a 2-channel tunable diode laser spectrometer for in-situ measurements of the atmospheric water vapor abundance near the ground, and improved instruments for measuring the relative abundances of oxygen isotopes (in water vapor and CO2) and a surface temperature probe for measuring the surface and sub-surface temperatures. This presentation will provide a brief overview of the environmental conditions anticipated at the surface in the Martian regions. We will then provide an over-view of the MVACS Met instrument and describe the MET sensors in detail, including their principle of operation, range, resolution, accuracy, sampling strategy, heritage, accommodation on the Lander, and their control and data handling system. Finally, we will describe the operational sequences, resource requirements, and the anticipated data volumes for each of the Met instruments.

Highly accurate FTIR observations from the scanning HIS aircraft instrument

NASA Astrophysics Data System (ADS)

Revercomb, Henry E.; Tobin, David C.; Knuteson, Robert O.; Best, Fred A.; Smith, William L., Sr.; van Delst, Paul F. W.; LaPorte, Daniel D.; Ellington, Scott D.; Werner, Mark W.; Dedecker, Ralph G.; Garcia, Raymond K.; Ciganovich, Nick N.; Howell, Hugh B.; Olson, Erik R.; Dutcher, Steven B.; Taylor, Joseph K.

2005-01-01

Development in the mid 80s of the High-resolution Interferometer Sounder (HIS) instrument for the high altitude NASA ER2 aircraft demonstrated the capability for advanced atmospheric temperature and water vapor sounding and set the stage for new satellite instruments that are now becoming a reality [AIRS(2002), CrIS(2006), IASI(2006), GIFTS(200?), HES(2013)]. Follow-on developments at the University of Wisconsin that employ Fourier Transform Infrared (FTIR) for Earth observations include the ground-based Atmospheric Emitted Radiance Interferometer (AERI) and the new Scanning HIS aircraft instrument. The Scanning HIS is a smaller version of the original HIS that uses cross-track scanning to enhance spatial coverage. Scanning HIS and its close cousin, the NPOESS Airborne Sounder Testbed (NAST), are being used for satellite instrument validation and for atmospheric research. A novel detector configuration on Scanning HIS allows the incorporation of a single focal plane and cooler with three or four spectral bands that view the same spot on the ground. The calibration accuracy of the S-HIS and results from recent field campaigns are presented, including validation comparisons with the NASA EOS infrared observations (AIRS and MODIS). Aircraft comparisons of this type provide a mechanism for periodically testing the absolute calibration of spacecraft instruments with instrumentation for which the calibration can be carefully maintained on the ground. This capability is especially valuable for assuring the long-term consistency and accuracy of climate observations, including those from the NASA EOS spacecrafts (Terra, Aqua and Aura) and the new complement of NPOESS operational instruments. It is expected that aircraft flights of the S-HIS and the NAST will be used to check the long-term stability of AIRS and the NPOESS operational follow-on sounder, the Cross-track Infrared Sounder (CrIS), over the life of the mission.

Experimental approaches to well controlled studies of thin-film nucleation and growth.

NASA Technical Reports Server (NTRS)

Poppa, H.; Moorhead, R. D.; Heinemann, K.

1972-01-01

Particular features and the performance of two experimental systems are described for quantitative studies of thin-film nucleation and growth processes including epitaxial depositions. System I consists of a modified LEED-Auger instrument combined with high-resolution electron microscopy. System II is a UHV electron microscope adapted for in-situ deposition studies. The two systems complement each other ideally, and the combined use of both can result in a comprehensive investigation of vapor deposition processes not obtainable with any other known method.

High Resolution Imager (HRI) for the Roentgen Satellite (ROSAT) definition study

NASA Technical Reports Server (NTRS)

1983-01-01

The design of the high resolution imager (HRI) on HEAO 2 was modified for use in the instrument complement of the Roentgen Satellite (ROSAT). Mechanical models of the front end assembly, central electronics assembly, and detector assembly were used to accurately represent the HRI envelope for both fit checks and focal plane configuration studies. The mechanical and electrical interfaces were defined and the requirements for electrical ground support equipment were established. A summary description of the ROSAT telescope and mission is included.

Advanced Instrumentation for Ultrafast Science at the LCLS

DOE Office of Scientific and Technical Information (OSTI.GOV)

Berrah, Nora

2015-10-13

This grant supported a Single Investigator and Small Group Research (SISGR) application to enable multi-user research in Ultrafast Science using the Linac Coherent Light Source (LCLS), the world’s first hard x-ray free electron laser (FEL) which lased for the first time at 1.5 Å on April 20, 2009. The goal of our proposal was to enable a New Era of Science by requesting funds to purchase and build Advanced Instrumentation for Ultrafast Science (AIUS), to utilize the intense, short x-ray pulses produced by the LCLS. The proposed instrumentation will allow peer review selected users to probe the ultrasmall and capture themore » ultrafast. These tools will expand on the investment already made in the construction of the light source and its instrumentation in both the LCLS and LUSI projects. The AIUS will provide researchers in the AMO, Chemical, Biological and Condensed Matter communities with greater flexibility in defining their scientific agenda at the LCLS. The proposed instrumentation will complement and significantly augment the present AMO instrument (funded through the LCLS project) through detectors and capabilities not included in the initial suite of instrumentation at the facility. We have built all of the instrumentations and they have been utilized by scientists. Please see report attached.« less

Measurement properties of quality-of-life measurement instruments for infants, children and adolescents with eczema: a systematic review.

PubMed

Heinl, D; Prinsen, C A C; Sach, T; Drucker, A M; Ofenloch, R; Flohr, C; Apfelbacher, C

2017-04-01

Quality of life (QoL) is one of the core outcome domains identified by the Harmonising Outcome Measures for Eczema (HOME) initiative to be assessed in every eczema trial. There is uncertainty about the most appropriate QoL instrument to measure this domain in infants, children and adolescents. To systematically evaluate the measurement properties of existing measurement instruments developed and/or validated for the measurement of QoL in infants, children and adolescents with eczema. A systematic literature search in PubMed and Embase, complemented by a thorough hand search of reference lists, retrieved studies on measurement properties of eczema QoL instruments for infants, children and adolescents. For all eligible studies, we judged the adequacy of the measurement properties and the methodological study quality with the COnsensus-based Standards for the selection of health Measurement INstruments (COSMIN) checklist. Results from different studies were summarized in a best-evidence synthesis and formed the basis to assign four degrees of recommendation. Seventeen articles, three of which were found by hand search, were included. These 17 articles reported on 24 instruments. No instrument can be recommended for use in all eczema trials because none fulfilled all required adequacy criteria. With adequate internal consistency, reliability and hypothesis testing, the U.S. version of the Childhood Atopic Dermatitis Impact Scale (CADIS), a proxy-reported instrument, has the potential to be recommended depending on the results of further validation studies. All other instruments, including all self-reported ones, lacked significant validation data. Currently, no QoL instrument for infants, children and adolescents with eczema can be highly recommended. Future validation research should primarily focus on the CADIS, but also attempt to broaden the evidence base for the validity of self-reported instruments. © 2016 British Association of Dermatologists.

Solar Terrestrial Relations Observatory (STEREO)

NASA Technical Reports Server (NTRS)

Davila, Joseph M.; SaintCyr, O. C.

2003-01-01

The solar magnetic field is constantly generated beneath the surface of the Sun by the solar dynamo. To balance this flux generation, there is constant dissipation of magnetic flux at and above the solar surface. The largest phenomenon associated with this dissipation is the Coronal Mass Ejection (CME). The Solar and Heliospheric Observatory (SOHO) has provided remarkable views of the corona and CMEs, and served to highlight how these large interplanetary disturbances can have terrestrial consequences. STEREO is the next logical step to study the physics of CME origin, propagation, and terrestrial effects. Two spacecraft with identical instrument complements will be launched on a single launch vehicle in November 2007. One spacecraft will drift ahead and the second behind the Earth at a separation rate of 22 degrees per year. Observation from these two vantage points will for the first time allow the observation of the three-dimensional structure of CMEs and the coronal structures where they originate. Each STEREO spacecraft carries a complement of 10 instruments, which include (for the first time) an extensive set of both remote sensing and in-situ instruments. The remote sensing suite is capable of imaging CMEs from the solar surface out to beyond Earth's orbit (1 AU), and in-situ instruments are able to measure distribution functions for electrons, protons, and ions over a broad energy range, from the normal thermal solar wind plasma to the most energetic solar particles. It is anticipated that these studies will ultimately lead to an increased understanding of the CME process and provide unique observations of the flow of energy from the corona to the near-Earth environment. An international research program, the International Heliophysical Year (IHY) will provide a framework for interpreting STEREO data in the context of global processes in the Sun-Earth system.

Development and Application of the Elementary School Science Classroom Environment Scale (ESSCES): Measuring Student Perceptions of Constructivism within the Science Classroom

ERIC Educational Resources Information Center

Peoples, Shelagh M.; O'Dwyer, Laura M.; Wang, Yang; Brown, Jessica J.; Rosca, Camelia V.

2014-01-01

This article describes the development, validation and application of a Rasch-based instrument, the Elementary School Science Classroom Environment Scale (ESSCES), for measuring students' perceptions of constructivist practices within the elementary science classroom. The instrument, designed to complement the Reformed Teaching Observation…

Validation and Verification (V and V) Testing on Midscale Flame Resistant (FR) Test Method

DTIC Science & Technology

2016-12-16

Method for Evaluation of Flame Resistant Clothing for Protection against Fire Simulations Using an Instrumented Manikin. Validation and...complement (not replace) the capabilities of the ASTM F1930 Standard Test Method for Evaluation of Flame Resistant Clothing for Protection against Fire ...Engineering Center (NSRDEC) to complement the ASTM F1930 Standard Test Method for Evaluation of Flame Resistant Clothing for Protection against Fire

Addressing continuous data measured with different instruments for participants excluded from trial analysis: a guide for systematic reviewers.

PubMed

Ebrahim, Shanil; Johnston, Bradley C; Akl, Elie A; Mustafa, Reem A; Sun, Xin; Walter, Stephen D; Heels-Ansdell, Diane; Alonso-Coello, Pablo; Guyatt, Gordon H

2014-05-01

We previously developed an approach to address the impact of missing participant data in meta-analyses of continuous variables in trials that used the same measurement instrument. We extend this approach to meta-analyses including trials that use different instruments to measure the same construct. We reviewed the available literature, conducted an iterative consultative process, and developed an approach involving a complete-case analysis complemented by sensitivity analyses that apply a series of increasingly stringent assumptions about results in patients with missing continuous outcome data. Our approach involves choosing the reference measurement instrument; converting scores from different instruments to the units of the reference instrument; developing four successively more stringent imputation strategies for addressing missing participant data; calculating a pooled mean difference for the complete-case analysis and imputation strategies; calculating the proportion of patients who experienced an important treatment effect; and judging the impact of the imputation strategies on the confidence in the estimate of effect. We applied our approach to an example systematic review of respiratory rehabilitation for chronic obstructive pulmonary disease. Our extended approach provides quantitative guidance for addressing missing participant data in systematic reviews of trials using different instruments to measure the same construct. Copyright © 2014 Elsevier Inc. All rights reserved.

Spectral Aerosol Extinction (SpEx): A New Instrument for In situ Ambient Aerosol Extinction Measurements Across the UV/Visible Wavelength Range

NASA Technical Reports Server (NTRS)

Jordan, C. E.; Anderson, B. E.; Beyersdorf, A. J.; Corr, C. A.; Dibb, J. E.; Greenslade, M. E.; Martin, R. F.; Moore, R. H.; Scheuer, E.; Shook, M. A.;

Measurement of nurses' attitudes and knowledge regarding acute care older patients: Psychometrics of the OPACS-US combined with the KOP-Q.

PubMed

Dikken, Jeroen; Hoogerduijn, Jita G; Lagerwey, Mary D; Shortridge-Baggett, Lillie; Klaassen, Sharon; Schuurmans, Marieke J

In clinical practice, identifying positive and negative attitudes toward older patients is very important to improve quality of care provided to them. The Older People in Acute Care Survey - United States (OPACS-US) is an instrument measuring hospital nurses attitudes regarding older patients. However, psychometrics have never been assessed. Furthermore, knowledge being related to attitude and behavior should also be measured complementing the OPACS-US. The purpose of this study was to assess structural validity and reliability of the OPACS-US and assess whether the OPACS-US can be complemented with the Knowledge about Older Patients-Quiz (KOP-Q). A multicenter cross sectional design was conducted. Registered nurses (n = 130, mean age 39,9 years; working experience 14,6 years) working in four general hospitals were included in the study. Nurses completed the OPACS-US section A: practice experiences, B: general opinion and the KOP-Q online. Findings demonstrated that the OPACS-US is a valid and reliable survey instrument that measures practice experiences and general opinion. Furthermore, the OPACS-US can be combined with the KOP-Q adding a knowledge construct, and is ready for use within education and/or quality improvement programs in the USA. Copyright © 2017 Elsevier Inc. All rights reserved.

Results of investigation at the Miravalles Geothermal Field, Costa Rica: Part 1, Well logging. Resultados de las investigaciones en el campo geotermico de Miravalles, Costa Rica: Parte 1, Registros de pozos (in EN;SP)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Dennis, B.R.; Lawton, R.G.; Kolar, J.D.

The well-logging operations performed in the Miravalles Geothermal Field in Costa Rica were conducted during two separate field trips. The Phase I program provided the deployment of a suite of high-temperature borehole instruments, including the temperature/rabbit, fluid sampler, and three-arm caliper in Well PGM-3. These same tools were deployed in Well PGM-10 along with an additional survey run with a combination fluid velocity/temperature/pressure instrument used to measure thermodynamic properties under flowing well conditions. The Phase II program complemented Phase I with the suite of tools deployed in Wells PGM-5, PGM-11, and PGM-12. 4 refs., 25 figs., 1 tab.

Atmospheric Laboratory for Applications and Science (ATLAS), mission 1: Introduction

NASA Technical Reports Server (NTRS)

1988-01-01

The first Atmospheric Laboratory for Applications and Science (ATLAS 1) is a NASA mission with an international payload, with the European Space Agency providing operational support for the European investigations. The ATLAS 1 represents the first of a series of shuttle-borne payloads which are intended to study the composition of the middle atmosphere and its possible variations due to solar changes over the course of an 11-year solar cycle. One of the ATLAS missions will coincide with NASA's Upper Atmospheric Research Satellite (UARS) mission and will provide crucial parameters not measured by the instrument complement on the satellite. A first in this evolutionary program, the ATLAS 1 will carry a payload of instruments originally flown on the Spacelab 1 and Spacelab 3 missions. The ATLAS mission therefore exploits the shuttle capability to return sophisticated instruments to the ground for refurbishment and updating, and the multi-mission reflight of the instruments at intervals required by the scientific goals. In addition to the investigations specific to the ATLAS objectives, the first mission payload includes others that are intended to study or use the near earth environment.

Economic rationality and health and lifestyle choices for people with diabetes.

PubMed

Baker, Rachel Mairi

2006-11-01

Economic rationality is traditionally represented by goal-oriented, maximising behaviour, or 'instrumental rationality'. Such a consequentialist, instrumental model of choice is often implicit in a biomedical approach to health promotion and education. The research reported here assesses the relevance of a broader conceptual framework of rationality, which includes 'procedural' and 'expressive' rationality as complements to an instrumental model of rationality, in a health context. Q methodology was used to derive 'factors' underlying health and lifestyle choices, based on a factor analysis of the results of a card sorting procedure undertaken by 27 adult respondents with type 2 diabetes in Newcastle upon Tyne, UK. These factors were then compared with the rationality framework and the appropriateness of an extended model of economic rationality as a means of better understanding health and lifestyle choices was assessed. Taking a wider rational choice perspective, choices which are rendered irrational within a narrow-biomedical or strictly instrumental model, can be understood in terms of a coherent rationale, grounded in the accounts of respondents. The implications of these findings are discussed in terms of rational choice theory and diabetes management and research.

Expected SOFIA sensitivity, characteristics, US science instrument complement and operations concept.

NASA Astrophysics Data System (ADS)

Becklin, E. E.; Davidson, J. A.

The joint US and German SOFIA project to develop and operate a 2.5 meter infrared airborne telescope in a Boeing 747-SP began earlier this year. Universities Space Research Association (USRA), teamed with Raytheon E-Systems and United Airlines, was selected by NASA to develop and operate SOFIA. The 2.5 meter telescope will be designed and built by a consortium of German companies lead by MAN-GHH. Work on the aircraft and the primary mirror has started. First science flights will begin in 2001 with 20% of the observing time assigned to German investigators. The observatory is expected to operate for over 20 years. The sensitivity, characteristics, US science instrument complement, and operations concept for the SOFIA observatory, with an emphasis on the science community's participation, are discussed.

[Evaluation of postoperative pain comparing manual and ultrasonic endodontic instrumentation in patients with cleft lip and palate].

PubMed

Brosco, H B; Pimentel, P A; Lacerda, A G; Nishiyama, C K; de Moraes, I G

1989-01-01

Our purpose was to compare incidence of post-surgical pain associated to the endodontic therapy where the instrumentation on the root canal was performed by the method of Marshall & Pappin and the method of Marshall & Pappin complemented by the ultrasonic. Seventy patients with only one tooth needing endodontic treatment were treated by one of the methods and, posteriorly, evaluated. The endodontic treatment was performed at one time and from the seventy teeth, thirty have been instrumented by the manual method complemented by the ultrasonic and forty by the manual instrumentation. The patients were clinically controlled after the endodontic treatment was finished during periods of 24, 48 and 72 hours to evaluate their post-surgical condition. The results suggest that were no statistically significant differences (p less than 0.05) in the incidence of pain between the employed methods or according to the pulpar semiologic condition in any of the observed periods. However, we have realized that there was a tendency for a smaller percentage of a postoperative pain in those cases of necropulpectamy treated by the endosonic ultrasonic synergistic system. In those cases of biopulpectomy this has been not observed.

Non-technical skills assessment for prelicensure nursing students: An integrative review.

PubMed

Pires, Sara; Monteiro, Sara; Pereira, Anabela; Chaló, Daniela; Melo, Elsa; Rodrigues, Alexandre

2017-11-01

In nursing, non-technical skills are recognized as playing an important role to increase patient safety and successful clinical outcomes (Pearson and McLafferty, 2011). Non-technical skills are cognitive and social resource skills that complement technical skills and contribute to safe and efficient task performance (Flin et al., 2008). In order to effectively provide non-technical skills training, it is essential to have an instrument to measure these skills. An online search was conducted. Articles were selected if they referred to and/or described instruments assessing non-technical skills for nurses and/or prelicensure nursing students in educational, clinical and/or simulated settings with validation evidence (inclusion criteria). Of the 53 articles located, 26 met the inclusion criteria. Those referred to and/or described 16 instruments with validation evidence developed to assess non-technical skills in multidisciplinary teams including nurses. Although articles have shown 16 valid and reliable instruments, to our knowledge, no instrument has been published or developed and validated for the assessment of non-technical skills of only nurses in general, relevant for use in high-fidelity simulation-based training for prelicensure nursing students. Therefore, there is a need for the development of such an instrument. Copyright © 2017 Elsevier Ltd. All rights reserved.

Self-administered health literacy instruments for people with diabetes: systematic review of measurement properties.

PubMed

Lee, Eun-Hyun; Kim, Chun-Ja; Lee, Jiyeon; Moon, Seung Hei

2017-09-01

The aims of this study were to identify all available self-administered instruments measuring health literacy in people with diabetes and to determine the current instrument that is the most appropriate for applying to this population in both practice and research. A systematic review of measurement properties. MEDLINE, EMBASE and CINAHL electronic databases from their inception up to 28 March 2016. The methodological quality of each included study was assessed using the COnsensus-based Standards for the selection of health Measurement INstruments checklist. The reported results for measurement properties in the studies were assessed according to Terwee's quality criteria. Thirteen self-administered instruments measuring health literacy in people with diabetes were identified, of which six (44%) were diabetes-specific instruments. The instruments that covered the broadest contents of health literacy were the Health Literacy Scale and Health Literacy Questionnaire. The (test-retest) reliability, measurement error and responsiveness were not evaluated for any instrument, while internal consistency and hypothesis testing validity were the most frequently assessed measurement properties. With the current evidence, the Health Literacy Scale may be the most appropriate instrument for patients with diabetes in practice and research. However, the structural validity of this scale needs to be further established, particularly in other language versions. It is also recommended to use the Diabetes Numeracy Test-15 along with the Health Literacy Scale to complement the lack of numeracy measures in the Health Literacy Scale. © 2017 John Wiley & Sons Ltd.

Joint analyses by laser-induced breakdown spectroscopy (LIBS) and Raman spectroscopy at stand-off distances.

PubMed

Wiens, Roger C; Sharma, Shiv K; Thompson, Justin; Misra, Anupam; Lucey, Paul G

2005-08-01

Raman spectroscopy and laser-induced breakdown spectroscopy (LIBS) of solid samples have both been shown to be feasible with sample-to-instrument distances of many meters. The two techniques are very useful together, as the combination of elemental compositions from LIBS and molecular vibrational information from Raman spectroscopy strongly complement each other. Remote LIBS and Raman spectroscopy spectra were taken together on a number of mineral samples including sulfates, carbonates and silicates at a distance of 8.3 m. The complementary nature of these spectra is highlighted and discussed. A factor of approximately 20 difference in intensity was observed between the brightest Raman line of calcite, at optimal laser power, and the brighter Ca I LIBS emission line measured with 55 mJ/pulse laser power. LIBS and Raman spectroscopy have several obstacles to devising a single instrument capable of both techniques. These include the differing spectral ranges and required detection sensitivity. The current state of technology in these areas is discussed.

The Combined Release and Radiation Effects Satellite (CRRES) program: A unique series of scientific experiments

NASA Technical Reports Server (NTRS)

Reasoner, David L.; Mccook, Morgan W. (Editor); Vaughan, William W. (Editor)

1990-01-01

The Defense Department and NASA have joined in a program to study the space environment which surrounds the earth and the effects of space radiation on modern satellite electronic systems. The Combined Release and Radiation Effects Satellite (CRRES) will carry an array of active experiments including chemical releases and a complement of sophisticated scientific instruments to accomplish these objectives. Other chemical release active experiments will be performed with sub-orbital rocket probes. The chemical releases will 'paint' the magnetic and electric fields of earthspace with clouds of glowing ions. Earthspace will be a laboratory, and the releases will be studied with an extensive network of ground-, aircraft-, and satellite-based diagnostic instruments. Some of the topics discussed include the following: the effects of earthspace; the need for active experiments; types of chemical releases; the CRRES program schedule; international support and coordinated studies; photographing chemical releases; information on locating chemical releases for observation by the amateur; and CRRES as a program.

The Combined Release and Radiation Effects Satellite (CRRES) program: A unique series of scientific experiments

NASA Astrophysics Data System (ADS)

Reasoner, David L.; McCook, Morgan W.; Vaughan, William W.

The Defense Department and NASA have joined in a program to study the space environment which surrounds the earth and the effects of space radiation on modern satellite electronic systems. The Combined Release and Radiation Effects Satellite (CRRES) will carry an array of active experiments including chemical releases and a complement of sophisticated scientific instruments to accomplish these objectives. Other chemical release active experiments will be performed with sub-orbital rocket probes. The chemical releases will 'paint' the magnetic and electric fields of earthspace with clouds of glowing ions. Earthspace will be a laboratory, and the releases will be studied with an extensive network of ground-, aircraft-, and satellite-based diagnostic instruments. Some of the topics discussed include the following: the effects of earthspace; the need for active experiments; types of chemical releases; the CRRES program schedule; international support and coordinated studies; photographing chemical releases; information on locating chemical releases for observation by the amateur; and CRRES as a program.

The Total In-Flight Simulator (TIFS) aerodynamics and systems: Description and analysis. [maneuver control and gust alleviators

NASA Technical Reports Server (NTRS)

Andrisani, D., II; Daughaday, H.; Dittenhauser, J.; Rynaski, E.

1978-01-01

The aerodynamics, control system, instrumentation complement and recording system of the USAF Total In/Flight Simulator (TIFS) airplane are described. A control system that would allow the ailerons to be operated collectively, as well as, differentially to entrance the ability of the vehicle to perform the dual function of maneuver load control and gust alleviation is emphasized. Mathematical prediction of the rigid body and the flexible equations of longitudinal motion using the level 2.01 FLEXSTAB program are included along with a definition of the vehicle geometry, the mass and stiffness distribution, the calculated mode frequencies and mode shapes, and the resulting aerodynamic equations of motion of the flexible vehicle. A complete description of the control and instrumentation system of the aircraft is presented, including analysis, ground test and flight data comparisons of the performance and bandwidth of the aerodynamic surface servos. Proposed modification for improved performance of the servos are also presented.

The Development of an Instrument to Measure Global Dimensions of Maternal Care in Rhesus Macaques (Macaca mulatta)

PubMed Central

McCormack, K.; Howell, B. R.; Guzman, D.; Villongco, C.; Pears, K.; Kim, H.; Gunnar, M.R.; Sanchez, M.M.

2014-01-01

One of the strongest predictors of healthy child development is the quality of maternal care. Although many measures of observation and self-report exist in humans to assess global aspects of maternal care, such qualitative measures are lacking in nonhuman primates. In this study we developed an instrument to measure global aspects of maternal care in rhesus monkeys, with the goal of complementing the individual behavioral data collected using a well-established rhesus macaque ethogram during the first months postpartum. The 22 items of the instrument were adapted from human maternal sensitivity assessments and a maternal Q-sort instrument already published for macaques. The 22 items formed four dimensions with high levels of internal reliability that represented major constructs of maternal care: 1) Sensitivity/Responsivity, 2) Protectiveness, 3) Permissiveness, and 4) Irritability. These dimensions yielded high construct validity when correlated with mother-infant frequency and duration behavior that was collected from focal observations across the first three postnatal months. In addition, comparisons of two groups of mothers (Maltreating versus Competent mothers), showed significant differences across the dimensions suggesting that this instrument has strong concurrent validity, even after controlling for focal observation variables that have been previously shown to significantly differentiate these groups. Our findings suggest that this Instrument of Macaque Maternal Care (IMMC) has the potential to capture global aspects of the mother-infant relationship that complement individual behaviors collected through focal observations. PMID:25066041

The development of an instrument to measure global dimensions of maternal care in rhesus macaques (Macaca mulatta).

PubMed

McCormack, K; Howell, B R; Guzman, D; Villongco, C; Pears, K; Kim, H; Gunnar, M R; Sanchez, M M

2015-01-01

One of the strongest predictors of healthy child development is the quality of maternal care. Although many measures of observation and self-report exist in humans to assess global aspects of maternal care, such qualitative measures are lacking in nonhuman primates. In this study, we developed an instrument to measure global aspects of maternal care in rhesus monkeys, with the goal of complementing the individual behavioral data collected using a well-established rhesus macaque ethogram during the first months postpartum. The 22 items of the instrument were adapted from human maternal sensitivity assessments and a maternal Q-sort instrument already published for macaques. The 22 items formed four dimensions with high levels of internal reliability that represented major constructs of maternal care: (1) Sensitivity/Responsivity, (2) Protectiveness, (3) Permissiveness, and (4) Irritability. These dimensions yielded high construct validity when correlated with mother-infant frequency and duration behavior that was collected from focal observations across the first 3 postnatal months. In addition, comparisons of two groups of mothers (Maltreating vs. Competent mothers) showed significant differences across the dimensions suggesting that this instrument has strong concurrent validity, even after controlling for focal observation variables that have been previously shown to significantly differentiate these groups. Our findings suggest that this Instrument of Macaque Maternal Care has the potential to capture global aspects of the mother-infant relationship that complement individual behaviors collected through focal observations. © 2014 Wiley Periodicals, Inc.

TFTR CAMAC systems and components

DOE Office of Scientific and Technical Information (OSTI.GOV)

Rauch, W.A.; Bergin, W.; Sichta, P.

1987-08-01

Princeton's tokamak fusion test reactor (TFTR) utilizes Computer Automated Measurement and Control (CAMAC) to provide instrumentation for real and quasi real time control, monitoring, and data acquisition systems. This paper describes and discusses the complement of CAMAC hardware systems and components that comprise the interface for tokamak control and measurement instrumentation, and communication with the central instrumentation control and data acquisition (CICADA) system. It also discusses CAMAC reliability and calibration, types of modules used, a summary of data acquisition and control points, and various diagnostic maintenance tools used to support and troubleshoot typical CAMAC systems on TFTR.

Solar Mesosphere Explorer optical-mechanical systems engineering

NASA Technical Reports Server (NTRS)

Gause, K. A.; Stuart, J. R.

1979-01-01

Mission overview of the Solar Mesosphere Explorer is presented along with design analysis and summaries of results. The Solar Mesosphere Explorer is a spin stabilized satellite carrying a complement of four Ebert-Fastie spectrometers and a four-channel Mersenne radiometer. Description of the spectrometer is given including a telescope and its aberrations. The radiometer is also described with consideration given to isothermal and thermal design, a Winston paraboloid, and optical tolerances. These five instruments are for measuring the earth's ozone density and distribution and providing quantitative data about those processes which govern the formation and destruction of ozone.

Mars Stratigraphy Mission

NASA Technical Reports Server (NTRS)

Budney, C. J.; Miller, S. L.; Cutts, J. A.

2000-01-01

The Mars Stratigraphy Mission lands a rover on the surface of Mars which descends down a cliff in Valles Marineris to study the stratigraphy. The rover carries a unique complement of instruments to analyze and age-date materials encountered during descent past 2 km of strata. The science objective for the Mars Stratigraphy Mission is to identify the geologic history of the layered deposits in the Valles Marineris region of Mars. This includes constraining the time interval for formation of these deposits by measuring the ages of various layers and determining the origin of the deposits (volcanic or sedimentary) by measuring their composition and imaging their morphology.

The New Cloud Absorption Radiometer (CAR) Software: One Model for NASA Remote Sensing Virtual Instruments

NASA Technical Reports Server (NTRS)

Roth, Don J.; Rapchun, David A.; Jones, Hollis H.

2001-01-01

The Cloud Absorption Radiometer (CAR) instrument has been the most frequently used airborne instrument built in-house at NASA Goddard Space Flight Center, having flown scientific research missions on-board various aircraft to many locations in the United States, Azores, Brazil, and Kuwait since 1983. The CAR instrument is capable of measuring scattered light by clouds in fourteen spectral bands in UV, visible and near-infrared region. This document describes the control, data acquisition, display, and file storage software for the new version of CAR. This software completely replaces the prior CAR Data System and Control Panel with a compact and robust virtual instrument computer interface. Additionally, the instrument is now usable for the first time for taking data in an off-aircraft mode. The new instrument is controlled via a LabVIEW v5. 1.1-developed software interface that utilizes, (1) serial port writes to write commands to the controller module of the instrument, and (2) serial port reads to acquire data from the controller module of the instrument. Step-by-step operational procedures are provided in this document. A suite of other software programs has been developed to complement the actual CAR virtual instrument. These programs include: (1) a simulator mode that allows pretesting of new features that might be added in the future, as well as demonstrations to CAR customers, and development at times when the instrument/hardware is off-location, and (2) a post-experiment data viewer that can be used to view all segments of individual data cycles and to locate positions where 'start' and stop' byte sequences were incorrectly formulated by the instrument controller. The CAR software described here is expected to be the basis for CAR operation for many missions and many years to come.

SOFIA - Stratospheric Observatory for Infrared Astronomy

NASA Astrophysics Data System (ADS)

Helton, A. L.; SOFIA Science Team

The Stratospheric Observatory for Infrared Astronomy (SOFIA) is a 2.7-m telescope mounted on board a Boeing 747-SP aircraft. Optimized for observations from infrared through sub-mm wavelengths, SOFIA observes from an altitude of 37,000 - 45,000 feet, above 99% of the atmospheric water vapor. The Observatory’s complement of instruments exhibits a broad range of capabilities that are well suited for the observation of dusty astronomical sources. During its first year of preliminary operations, SOFIA made a number of exciting observations, including the discovery of a new high-mass protostar in the Orion Nebula (IRc4), the first detection of OD outside our Solar System, the detection of interstellar mercapto radicals (SH), and some of the highest resolution mid-IR observations of the transient Galactic circumnuclear ring to date. Here we present a selection of the available instruments available on board SOFIA and discuss their potential for future studies of dust in the Universe.

Field trial of the enhanced data authentication system (EDAS)

DOE PAGES

Thomas, Maikael A.; Hymel, Ross W.; Baldwin, George; ...

2016-11-01

The Enhanced Data Authentication System (EDAS) is means to securely branch information from an existing measurement system or data stream to a secondary observer. In an international nuclear safeguards context, the EDAS connects to operator instrumentation, and provides a cryptographically secure copy of the information for a safeguards inspectorate. However, this novel capability could be a valuable complement to inspector-owned safeguards instrumentation, offering context that is valuable for anomaly resolution and contingency.

Old Pianos--Junque or Jewels.

ERIC Educational Resources Information Center

Maxwell, David

1981-01-01

Presents criteria for evaluating a used piano which, complemented by a qualified piano technician's assessment and cost estimates for needed repairs, will allow a music director to decide whether it is more cost-effective to repair or discard the instrument. (Author/SJL)

General Astrophysics Science Enabled by the HabEx Ultraviolet Spectrograph (UVS)

NASA Astrophysics Data System (ADS)

Scowen, Paul; Clarke, John; Gaudi, B. Scott; Kiessling, Alina; Martin, Stefan; Somerville, Rachel; Stern, Daniel; HabEx Science and Technology Definition Team

2018-01-01

The Habitable Exoplanet Imaging Mission (HabEx) is one of the four large mission concepts being studied by NASA as input to the upcoming 2020 Decadal Survey. The mission implements two world-class General Astrophysics instruments as part of its complement of instrumentation to enable compelling science using the 4m aperture. The Ultraviolet Spectrograph has been designed to address cutting edge far ultraviolet (FUV) science that has not been possible with the Hubble Space Telescope, and to open up a wide range of capabilities that will advance astrophysics as we look into the 2030s. Our poster discusses some of those science drivers and possible applications, which range from Solar System science, to nearby and more distant studies of star formation, to studies of the circumgalactic and intergalactic mediums where the ecology of mass and energy transfer are vital to understanding stellar and galactic evolution. We discuss the performance features of the instrument that include a large 3’x3’ field of view for multi-object spectroscopy, and some 20 grating modes for a variety of spectral resolution and coverage.

An Imaging Calorimeter for Access-Concept Study

NASA Technical Reports Server (NTRS)

Parnell, T. A.; Adams, James H.; Binns, R. W.; Christl, M. J.; Derrickson, J. H.; Fountain, W. F.; Howell, L. W.; Gregory, J. C.; Hink, P. L.; Israel, M. H.;

Cosmochemistry: Understanding the Solar System through analysis of extraterrestrial materials.

PubMed

MacPherson, Glenn J; Thiemens, Mark H

2011-11-29

Cosmochemistry is the chemical analysis of extraterrestrial materials. This term generally is taken to mean laboratory analysis, which is the cosmochemistry gold standard because of the ability for repeated analysis under highly controlled conditions using the most advanced instrumentation unhindered by limitations in power, space, or environment. Over the past 40 y, advances in technology have enabled telescopic and spacecraft instruments to provide important data that significantly complement the laboratory data. In this special edition, recent advances in the state of the art of cosmochemistry are presented, which range from instrumental analysis of meteorites to theoretical-computational and astronomical observations.

In Situ Strategy of the 2011 Mars Science Laboratory to Investigate the Habitability of Ancient Mars

NASA Technical Reports Server (NTRS)

Mahaffy, Paul R.

2011-01-01

The ten science investigations of the 2011 Mars Science Laboratory (MSL) Rover named "Curiosity" seek to provide a quantitative assessment of habitability through chemical and geological measurements from a highly capable robotic' platform. This mission seeks to understand if the conditions for life on ancient Mars are preserved in the near-surface geochemical record. These substantial payload resources enabled by MSL's new entry descent and landing (EDL) system have allowed the inclusion of instrument types nevv to the Mars surface including those that can accept delivered sample from rocks and soils and perform a wide range of chemical, isotopic, and mineralogical analyses. The Chemistry and Mineralogy (CheMin) experiment that is located in the interior of the rover is a powder x-ray Diffraction (XRD) and X-ray Fluorescence (XRF) instrument that provides elemental and mineralogical information. The Sample Analysis at Mars (SAM) suite of instruments complements this experiment by analyzing the volatile component of identically processed samples and by analyzing atmospheric composition. Other MSL payload tools such as the Mast Camera (Mastcam) and the Chemistry & Camera (ChemCam) instruments are utilized to identify targets for interrogation first by the arm tools and subsequent ingestion into SAM and CheMin using the Sample Acquisition, Processing, and Handling (SA/SPaH) subsystem. The arm tools include the Mars Hand Lens Imager (MAHLI) and the Chemistry and Alpha Particle X-ray Spectrometer (APXX). The Dynamic Albedo of Neutrons (DAN) instrument provides subsurface identification of hydrogen such as that contained in hydrated minerals

A Radionavigation Systems Course

ERIC Educational Resources Information Center

Lozano-Guerrero, Antonio José; Valenzuela-Valdés, Juan Francisco

2015-01-01

This paper presents a new course, Radionavigation Systems, whose laboratory and theoretical components complement each other to enhance student learning. Radionavigation skills and knowledge are taught by means of various instructional methods, and the laboratory successfully merges hands-on learning using specific instrumentation and software…

Contributed Review: Nuclear magnetic resonance core analysis at 0.3 T

NASA Astrophysics Data System (ADS)

Mitchell, Jonathan; Fordham, Edmund J.

2014-11-01

Nuclear magnetic resonance (NMR) provides a powerful toolbox for petrophysical characterization of reservoir core plugs and fluids in the laboratory. Previously, there has been considerable focus on low field magnet technology for well log calibration. Now there is renewed interest in the study of reservoir samples using stronger magnets to complement these standard NMR measurements. Here, the capabilities of an imaging magnet with a field strength of 0.3 T (corresponding to 12.9 MHz for proton) are reviewed in the context of reservoir core analysis. Quantitative estimates of porosity (saturation) and pore size distributions are obtained under favorable conditions (e.g., in carbonates), with the added advantage of multidimensional imaging, detection of lower gyromagnetic ratio nuclei, and short probe recovery times that make the system suitable for shale studies. Intermediate field instruments provide quantitative porosity maps of rock plugs that cannot be obtained using high field medical scanners due to the field-dependent susceptibility contrast in the porous medium. Example data are presented that highlight the potential applications of an intermediate field imaging instrument as a complement to low field instruments in core analysis and for materials science studies in general.

Teaching Confirmatory Factor Analysis to Non-Statisticians: A Case Study for Estimating Composite Reliability of Psychometric Instruments

PubMed Central

Gajewski, Byron J.; Jiang, Yu; Yeh, Hung-Wen; Engelman, Kimberly; Teel, Cynthia; Choi, Won S.; Greiner, K. Allen; Daley, Christine Makosky

2013-01-01

Texts and software that we are currently using for teaching multivariate analysis to non-statisticians lack in the delivery of confirmatory factor analysis (CFA). The purpose of this paper is to provide educators with a complement to these resources that includes CFA and its computation. We focus on how to use CFA to estimate a “composite reliability” of a psychometric instrument. This paper provides guidance for introducing, via a case-study, the non-statistician to CFA. As a complement to our instruction about the more traditional SPSS, we successfully piloted the software R for estimating CFA on nine non-statisticians. This approach can be used with healthcare graduate students taking a multivariate course, as well as modified for community stakeholders of our Center for American Indian Community Health (e.g. community advisory boards, summer interns, & research team members). The placement of CFA at the end of the class is strategic and gives us an opportunity to do some innovative teaching: (1) build ideas for understanding the case study using previous course work (such as ANOVA); (2) incorporate multi-dimensional scaling (that students already learned) into the selection of a factor structure (new concept); (3) use interactive data from the students (active learning); (4) review matrix algebra and its importance to psychometric evaluation; (5) show students how to do the calculation on their own; and (6) give students access to an actual recent research project. PMID:24772373

Elements of NASA GSFC Wallops Island's ozone measurement program

NASA Astrophysics Data System (ADS)

Schmidlin, F. J.; Schauer, A. G.; Thompson, A. M.; Northam, E. T.; Brothers, G. B.; Beebe, A.

2003-04-01

Ozone observations from GSFC Wallops Flight Facility, Natal, Brazil, and Ascension Island involve instrument preparation, analyses, comparisons, archiving, and documentation. The complement of instrumentation enables reliable, accurate world-class information be made available to the scientific community. At Wallops Island, instruments in use include the electrochemical concentration cell ECC ozonesondes, Dobson spectrophotometer, Microtops Sun Photometer, Ground-based Ultraviolet Radiometer GUV, and the NILU-UV Irradiance meter. ECC's and a Dobson are used at Natal while ECC's and a handheld Microtops Sunphotometer are used at Ascension Island. ECC ozonesondes are released from Natal as part of an agreement between NASA and INPE and from Ascension Island with US Air Force cooperation. Both of these sites provide vertical ozone profiles to SHADOZ. All of the instruments mentioned are widely used and need not be described further. Unique ECC preparation procedures developed at Wallops Island over many years also are used at the three sites. Description of the ECC calibration against known standards is given. Emphasis is given to results of comparisons between ECC's of two manufacturers, to the affect of different KI solutions and possible adjustment to older measurements that used different KI solution strengths, and to laboratory tests conducted during JOSIE2000 and their relationship to in situ tests conducted at Wallops Island. If time permits, we will give a summary of the performance of the ground-based instruments.

Review of 99 self-report measures for assessing well-being in adults: exploring dimensions of well-being and developments over time

PubMed Central

Dieppe, Paul

2016-01-01

Objective Investigators within many disciplines are using measures of well-being, but it is not always clear what they are measuring, or which instruments may best meet their objectives. The aims of this review were to: systematically identify well-being instruments, explore the variety of well-being dimensions within instruments and describe how the production of instruments has developed over time. Design Systematic searches, thematic analysis and narrative synthesis were undertaken. Data sources MEDLINE, EMBASE, EconLit, PsycINFO, Cochrane Library and CINAHL from 1993 to 2014 complemented by web searches and expert consultations through 2015. Eligibility criteria Instruments were selected for review if they were designed for adults (≥18 years old), generic (ie, non-disease or context specific) and available in an English version. Results A total of 99 measures of well-being were included, and 196 dimensions of well-being were identified within them. Dimensions clustered around 6 key thematic domains: mental well-being, social well-being, physical well-being, spiritual well-being, activities and functioning, and personal circumstances. Authors were rarely explicit about how existing theories had influenced the design of their tools; however, the 2 most referenced theories were Diener's model of subjective well-being and the WHO definition of health. The period between 1990 and 1999 produced the greatest number of newly developed well-being instruments (n=27). An illustration of the dimensions identified and the instruments that measure them is provided within a thematic framework of well-being. Conclusions This review provides researchers with an organised toolkit of instruments, dimensions and an accompanying glossary. The striking variability between instruments supports the need to pay close attention to what is being assessed under the umbrella of ‘well-being’ measurement. PMID:27388349

Validation of Atmospheric InfraRed Sounder (AIRS) spectral radiances with the Scanning High-resolution Interferometer Sounder (S-HIS) aircraft instrument

NASA Astrophysics Data System (ADS)

Tobin, David C.; Revercomb, Henry E.; Moeller, Chris C.; Knuteson, Robert O.; Best, Fred A.; Smith, William L.; van Delst, Paul; LaPorte, Daniel D.; Ellington, Scott D.; Werner, Mark D.; Dedecker, Ralph G.; Garcia, Raymond K.; Ciganovich, Nick N.; Howell, Hugh B.; Dutcher, Steven B.; Taylor, Joe K.

2004-11-01

The ability to accurately validate high spectral resolution infrared radiance measurements from space using comparisons with aircraft spectrometer observations has been successfully demonstrated. The demonstration is based on an under-flight of the Atmospheric Infrared Sounder (AIRS) on the NASA Aqua spacecraft by the Scanning High resolution Interferometer Sounder (S-HIS) on the NASA ER-2 high altitude aircraft on 21 November 2002 and resulted in brightness temperature differences approaching 0.1K for most of the spectrum. This paper presents the details of this AIRS/S-HIS validation case and also presents comparisons of Aqua AIRS and Moderate Resolution Imaging Spectroradiometer (MODIS) radiance observations. Aircraft comparisons of this type provide a mechanism for periodically testing the absolute calibration of spacecraft instruments with instrumentation for which the calibration can be carefully maintained on the ground. This capability is especially valuable for assuring the long-term consistency and accuracy of climate observations. It is expected that aircraft flights of the S-HIS and its close cousin the National Polar Orbiting Environmental Satellite System (NPOESS) Atmospheric Sounder Testbed (NAST) will be used to check the long-term stability of the NASA EOS spacecrafts (Terra, Aqua and Aura) and the follow-on complement of operational instruments, including the Cross-track Infrared Sounder (CrIS).

The Role of APEX as a Pathfinder for AtLAST

NASA Astrophysics Data System (ADS)

Wyrowski, Friedrich

2018-01-01

Now more than 12 years in operation, the Atacama Pathfinder Experiment (APEX) 12 m submillimeter telescope has significantly contributed to a wide variety of submillimeter astronomy science areas, ranging from the discoveries of new molecules to large and deep imaging of the submillimeter sky. While ALMA operation is in full swing, APEX is strengthening its role not only as pathfinder for studying large source samples and spatial scales to prepare detailed high angular resolution ALMA follow ups, but also as fast response instruments to complement new results from ALMA. Furthermore, APEX ensures southern hemisphere access for submillimeter projects complementing archival Herschel research as well as new SOFIA science. With new broadband and multipixel receivers as well as large cameras for wide-field continuum imaging, APEX will pave the way towards the science envisioned with ATLAST. In this contribution, the current status and ongoing upgrades of APEX will be discussed, with an emphasis on the importance of continuous cutting edge science and state-of-the-art instrumentation that will bridge the gap towards ATLAST.

Cryo-Vacuum Testing of the JWST Integrated Science Instrument Module

NASA Technical Reports Server (NTRS)

Kimble, Randy A.; Vila, M. Begona; Van Campen, Julie M.; Birkmann, Stephen M.; Comber, Brian J.; Fatig, Curtis C.; Glasse, Alistair C. H.; Glazer, Stuart D.; Kelly, Douglas M.; Mann, Steven D.;

Cosmochemistry: Understanding the Solar System through analysis of extraterrestrial materials

PubMed Central

MacPherson, Glenn J.; Thiemens, Mark H.

2011-01-01

Cosmochemistry is the chemical analysis of extraterrestrial materials. This term generally is taken to mean laboratory analysis, which is the cosmochemistry gold standard because of the ability for repeated analysis under highly controlled conditions using the most advanced instrumentation unhindered by limitations in power, space, or environment. Over the past 40 y, advances in technology have enabled telescopic and spacecraft instruments to provide important data that significantly complement the laboratory data. In this special edition, recent advances in the state of the art of cosmochemistry are presented, which range from instrumental analysis of meteorites to theoretical–computational and astronomical observations. PMID:22128323

Complement Evasion Strategies of Viruses: An Overview

PubMed Central

Agrawal, Palak; Nawadkar, Renuka; Ojha, Hina; Kumar, Jitendra; Sahu, Arvind

2017-01-01

Being a major first line of immune defense, the complement system keeps a constant vigil against viruses. Its ability to recognize large panoply of viruses and virus-infected cells, and trigger the effector pathways, results in neutralization of viruses and killing of the infected cells. This selection pressure exerted by complement on viruses has made them evolve a multitude of countermeasures. These include targeting the recognition molecules for the avoidance of detection, targeting key enzymes and complexes of the complement pathways like C3 convertases and C5b-9 formation – either by encoding complement regulators or by recruiting membrane-bound and soluble host complement regulators, cleaving complement proteins by encoding protease, and inhibiting the synthesis of complement proteins. Additionally, viruses also exploit the complement system for their own benefit. For example, they use complement receptors as well as membrane regulators for cellular entry as well as their spread. Here, we provide an overview on the complement subversion mechanisms adopted by the members of various viral families including Poxviridae, Herpesviridae, Adenoviridae, Flaviviridae, Retroviridae, Picornaviridae, Astroviridae, Togaviridae, Orthomyxoviridae and Paramyxoviridae. PMID:28670306

Documenting Collections: Cornerstones for More History of Science in Museums

ERIC Educational Resources Information Center

Lourenço, Marta C.; Gessner, Samuel

2014-01-01

Historians of science have recently become increasingly involved with collections and scientific instruments. This creates opportunities for a more significant role of history in museums of science, as well as more meaningful and contextualized exhibitions and educational programmes. However, complementing the mainstream focus on universal…

VIMOS Instrument Control Software Design: an Object Oriented Approach

NASA Astrophysics Data System (ADS)

Brau-Nogué, Sylvie; Lucuix, Christian

2002-12-01

The Franco-Italian VIMOS instrument is a VIsible imaging Multi-Object Spectrograph with outstanding multiplex capabilities, allowing to take spectra of more than 800 objects simultaneously, or integral field spectroscopy mode in a 54x54 arcsec area. VIMOS is being installed at the Nasmyth focus of the third Unit Telescope of the European Southern Observatory Very Large Telescope (VLT) at Mount Paranal in Chile. This paper will describe the analysis, the design and the implementation of the VIMOS Instrument Control System, using UML notation. Our Control group followed an Object Oriented software process while keeping in mind the ESO VLT standard control concepts. At ESO VLT a complete software library is available. Rather than applying waterfall lifecycle, ICS project used iterative development, a lifecycle consisting of several iterations. Each iteration consisted in : capture and evaluate the requirements, visual modeling for analysis and design, implementation, test, and deployment. Depending of the project phases, iterations focused more or less on specific activity. The result is an object model (the design model), including use-case realizations. An implementation view and a deployment view complement this product. An extract of VIMOS ICS UML model will be presented and some implementation, integration and test issues will be discussed.

Highly integrated Pluto payload system (HIPPS): a sciencecraft instrument for the Pluto mission

NASA Astrophysics Data System (ADS)

Stern, S. Alan; Slater, David C.; Gibson, William; Reitsema, Harold J.; Delamere, W. Alan; Jennings, Donald E.; Reuter, D. C.; Clarke, John T.; Porco, Carolyn C.; Shoemaker, Eugene M.; Spencer, John R.

1995-09-01

We describe the design concept for the highly integrated Pluto payload system (HIPPS): a highly integrated, low-cost, light-weight, low-power instrument payload designed to fly aboard the proposed NASA Pluto flyby spacecraft destined for the Pluto/Charon system. The HIPPS payload is designed to accomplish all of the Pluto flyby prime (IA) science objectives, except radio science, set forth by NASA's Outer Planets Science Working Group (OPSWG) and the Pluto Express Science Definition Team (SDT). HIPPS contains a complement of three instrument components within one common infrastructure; these are: (1) a visible/near UV CCD imaging camera; (2) an infrared spectrograph; and (3) an ultraviolet spectrograph. A detailed description of each instrument is presented along with how they will meet the IA science requirements.

Titan Science with the James Webb Space Telescope

NASA Technical Reports Server (NTRS)

Nixon, Conor A.; Achterberg, Richard K.; Adamkovics, Mate; Bezard, Bruno; Bjoraker, Gordon L.; Comet, Thomas; Hayes, Alaxander G.; Lellouch, Emmanuel; Lemmon, Mark T.; Lopez-Puertas, Manuel;

Titan Science with the James Webb Space Telescope

NASA Astrophysics Data System (ADS)

Nixon, Conor A.; Achterberg, Richard K.; Ádámkovics, Máté; Bézard, Bruno; Bjoraker, Gordon L.; Cornet, Thomas; Hayes, Alexander G.; Lellouch, Emmanuel; Lemmon, Mark T.; López-Puertas, Manuel; Rodriguez, Sébastien; Sotin, Christophe; Teanby, Nicholas A.; Turtle, Elizabeth P.; West, Robert A.

2016-01-01

The James Webb Space Telescope (JWST), scheduled for launch in 2018, is the successor to the Hubble Space Telescope (HST) but with a significantly larger aperture (6.5 m) and advanced instrumentation focusing on infrared science (0.6-28.0 μm). In this paper, we examine the potential for scientific investigation of Titan using JWST, primarily with three of the four instruments: NIRSpec, NIRCam, and MIRI, noting that science with NIRISS will be complementary. Five core scientific themes are identified: (1) surface (2) tropospheric clouds (3) tropospheric gases (4) stratospheric composition, and (5) stratospheric hazes. We discuss each theme in depth, including the scientific purpose, capabilities, and limitations of the instrument suite and suggested observing schemes. We pay particular attention to saturation, which is a problem for all three instruments, but may be alleviated for NIRCam through use of selecting small sub-arrays of the detectors—sufficient to encompass Titan, but with significantly faster readout times. We find that JWST has very significant potential for advancing Titan science, with a spectral resolution exceeding the Cassini instrument suite at near-infrared wavelengths and a spatial resolution exceeding HST at the same wavelengths. In particular, JWST will be valuable for time-domain monitoring of Titan, given a five- to ten-year expected lifetime for the observatory, for example, monitoring the seasonal appearance of clouds. JWST observations in the post-Cassini period will complement those of other large facilities such as HST, ALMA, SOFIA, and next-generation ground-based telescopes (TMT, GMT, EELT).

Stratospheric observatory for infrared astronomy (SOFIA)

NASA Astrophysics Data System (ADS)

Becklin, E. E.; Moon, L. J.

The joint U.S. and German SOFIA project to develop and operate a 2.5-meter infrared airborne telescope in a Boeing 747-SP is now well into development. First science flights will begin in 2004 with 20% of the observing time assigned to German investigators. The observatory is expected to operate for over 20 years. The sensitivity, characteristics and science instrument complement are discussed. SOFIA will have instrumentation that will allow astronomical surveys that were not possible on the KAO. A future SOFIA survey project related to astrochemistry is discussed.

Stratospheric Observatory for Infrared Astronomy (SOFIA)

NASA Astrophysics Data System (ADS)

Becklin, E. E.

1999-03-01

The joint US and German SOFIA project to develop and operate a 2.5 meter infrared airborne telescope in a Boeing 747-SP is now in full development. Work on the aircraft and the primary mirror has started. First science flights will begin in 2001 with 20 per cent of the observing time assigned to German investigators. The observatory is expected to operate for over 20 years. The sensitivity, characteristics and science instrument complement are discussed. SOFIA will have facility instrumentation that will allow much more use by scientists than was possible on the KAO.

The Large Ultraviolet/Optical/Infrared Surveyor (LUVOIR)

NASA Astrophysics Data System (ADS)

Peterson, Bradley M.; Fischer, Debra; LUVOIR Science and Technology Definition Team

2017-01-01

LUVOIR is one of four potential large mission concepts for which the NASA Astrophysics Division has commissioned studies by Science and Technology Definition Teams (STDTs) drawn from the astronomical community. LUVOIR will have an 8 to16-m segmented primary mirror and operate at the Sun-Earth L2 point. It will be designed to support a broad range of astrophysics and exoplanet studies. The notional initial complement of instruments will include 1) a high-performance optical/NIR coronagraph with imaging and spectroscopic capability, 2) a UV imager and spectrograph with high spectral resolution and multi-object capability, 3) a high-definition wide-field optical/NIR camera, and 4) a multi-resolution optical/NIR spectrograph. LUVOIR will be designed for extreme stability to support unprecedented spatial resolution and coronagraphy. It is intended to be a long-lifetime facility that is both serviceable and upgradable. This is the first report by the LUVOIR STDT to the community on the top-level architectures we are studying, including preliminary capabilities of a mission with those parameters. The STDT seeks feedback from the astronomical community for key science investigations that can be undertaken with the notional instrument suite and to identify desirable capabilities that will enable additional key science.

Final Report: High Spectral Resolution Atmospheric Emitted Radiance Studies with the ARM UAV

DOE Office of Scientific and Technical Information (OSTI.GOV)

Revercomb, Henry E.

1999-12-31

The active participation in the Atmospheric Radiation Measurement (ARM) Unmanned Airborne Vehicle (UAV) science team that was anticipated in the grant proposal was indefinitely delayed after the first year due to a programmatic decision to exclude the high spectral resolution observations from the existing ARM UAV program. However, this report shows that substantial progress toward the science objectives of this grant have made with the help of separate funding from NASA and other agencies. In the four year grant period (including time extensions), a new high spectral resolution instrument has been flown and has successfully demonstrated the ability to obtainmore » measurements of the type needed in the conduct of this grant. In the near term, the third water vapor intensive observing period (WVIOP-3) in October 2000 will provide an opportunity to bring the high spectral resolution observations of upwelling radiance into the ARM program to complement the downwelling radiance observations from the existing ARM AERI instruments. We look forward to a time when the ARM-UAV program is able to extend its scope to include the capability for making these high spectral resolution measurements from a UAV platform.« less

Earth remote sensing with NPOESS: instruments and environmental data products

NASA Astrophysics Data System (ADS)

Glackin, David L.; Cunningham, John D.; Nelson, Craig S.

2004-02-01

The NPOESS (National Polar-orbiting Operational Environmental Satellite System) program represents the merger of the NOAA POES (Polar-orbiting Environmental Satellite) program and the DoD DMSP (Defense Meteorological Satellite Program) satellites. Established by presidential directive in 1994, a tri-agency Integrated Program Office (IPO) in Silver Spring, Maryland, has been managing NPOESS development, and is staffed by representatives of NOAA, DoD, and NASA. NPOESS is being designed to provide 55 atmospheric, oceanographic, terrestrial, and solar-geophysical data products, and will disseminate them to civilian and military users worldwide. The first NPOESS satellite is scheduled to be launched late in this decade, with the other two satellites of the three-satellite constellation due to be launched over the ensuing four years. NPOESS will remain operational for at least ten years. The 55 Environmental Data Records (EDRs) will be provided by a number of instruments, many of which will be briefly described in this paper. The instruments will be hosted in various combinations on three NPOESS platforms in three distinct polar sun-synchronous orbits. The instrument complement represents the combined requirements of the weather, climate, and environmental remote sensing communities. The three critical instruments are VIIRS (Visible/Infrared Imager-Radiometer Suite), CMIS (Conical Microwave Imager/Sounder), and CrIS (Cross-track Infrared Sounder). The other IPO-developed instruments are OMPS (Ozone Mapper/Profiler Suite), GPSOS (Global Positioning System Occultation Sensor), the APS (Aerosol Polarimeter Sensor), and the SESS (Space Environment Sensor Suite). NPOESS will also carry various "leveraged" instruments, i.e., ones that do not require development by the IPO. These include the ATMS (Advanced Technology Microwave Sounder), the TSIS (Total Solar Irradiance Sensor), the ERBS (Earth Radiation Budget Sensor), and the ALT (Radar Altimeter).

MEDA, The New Instrument for Mars Environment Analysis for the Mars 2020 Mission

NASA Astrophysics Data System (ADS)

Moreno-Alvarez, Jose F.; Pena-Godino, Antonio; Rodriguez-Manfredi, Jose Antonio; Cordoba, Elizabeth; MEDA Team

2016-08-01

The Mars 2020 rover mission is part of NASA's Mars Exploration Program, a long-term effort of robotic exploration of the red planet. Designed to advance high-priority science goals for Mars exploration, the mission will address key questions about the potential for life on Mars. The mission will also provide opportunities to gather knowledge and demonstrate technologies that address the challenges of future human expeditions to Mars.The Mars Environmental Dynamics Analyzer (MEDA) is an integrated full suite of sensors designed to address the Mars 2020 mission objectives of characterization of dust size and morphology and surface weather measurements.MEDA system consists of one control unit and 10 separated sensor enclosures distributed in different positions along the Mars 2020 rover. MEDA is composed of an ARM-based control computer with its flight software application, two wind sensors including mixed ASICs inside, five air temperature sensors, one sky pointing camera complemented with 16 photo- detectors looking up and around, one thermal infrared sensor using five measurement bands, one relative humidity sensor, one pressure sensor and the harness that interconnects all of them. It is a complex system intended to operate in one of the harshest environments possible, the Mars surface, for many years to come.This will become a short term reality thanks to the combination of a strong international science team driving the science and system requirements working together with a powerful industrial organization to design and build the instrument. The instrument is being built right now, with its Critical Design Review at the end of 2016, and the flight model to be provided in 2018.This paper summarizes the main scientific objective of the MEDA instrument, the links between the Mission and the MEDA science objectives, and the challenging environmental Mars requirements. It will then focus on the engineered definition of the instrument, showing the overall architecture of the instrument and its sensors, including a discussion of the heritage from REMS.

Educators' Perceptions of School Climate and Health in Selected Primary Schools

ERIC Educational Resources Information Center

Pretorius, Stephanus; de Villiers, Elsabe

2009-01-01

The aims in this research were to determine the perceptions of school climate held by educators of primary schools in the southern Cape. Six primary schools with a staff complement of 178 educators participated in the investigation. Two instruments were used: the Organisational Climate Description Questionnaire Rutgers Elementary (OCDQ-RE) and…

A study of system requirements for Phobos/Diemos missions. Volume 3: Phase 2 results, satellite sample return missions and satellite mobility concepts

NASA Technical Reports Server (NTRS)

1972-01-01

The scientific objectives were considered for a Phobos/Deimos mission. The payloads for a minimum useful instrument complement were developed. The rationale for a sample return mission is discussed, along with the scientific constraints and requirements for the acquisition of samples.

Evaluation of Flexibility Under "No Child Left Behind": Volume I--Executive Summary of Transferability, REAP Flex, and Local-Flex Evaluations

ERIC Educational Resources Information Center

Christensen, Gayle S.

2007-01-01

The reauthorization of the "Elementary and Secondary Education Act" ("ESEA") as amended by the "No Child Left Behind Act of 2001" ("NCLB") relied on two notable policy instruments to improve education: accountability and flexibility. "NCLB" complements accountability with several new flexibility…

Questioning LibQUAL+[TM]: Expanding Its Assessment of Academic Library Effectiveness

ERIC Educational Resources Information Center

Edgar, William B.

2006-01-01

This article examines LibQUAL+[TM]'s instrument, fundamental assumption, and research approach and proposes a functional/technical model of academic library effectiveness. This expanded view of library effectiveness complements LibQUAL+[TM], emphasizing it to be dependent upon users' experience of service delivery, as LibQUAL+[TM] recognizes.…

JPSS Products, Applications and Training

NASA Astrophysics Data System (ADS)

Torres, J. R.; Connell, B. H.; Miller, S. D.

2017-12-01

The Joint Polar Satellite System (JPSS) is a new generation polar-orbiting operational environmental satellite system that will monitor the weather and environment around the globe. JPSS will provide technological and scientific improvements in environmental monitoring via high resolution satellite imagery and derived products that stand to improve weather forecasting capabilities for National Weather Service (NWS) forecasters and complement operational Geostationary satellites. JPSS will consist of four satellites, JPSS-1 through JPSS-4, where JPSS-1 is due to launch in Fall 2017. A predecessor, prototype and operational risk-reduction for JPSS is the Suomi-National Polar-orbiting Partnership (S-NPP) satellite, launched on 28 October 2011. The following instruments on-board S-NPP will also be hosted on JPSS-1: Visible Infrared Imaging Radiometer Suite (VIIRS), Cross-track Infrared Sounder (CrIS), Advanced Technology Microwave Sounder (ATMS), Ozone Mapping and Profiler Suite (OMPS) and the Clouds and Earth's Radiant Energy System (CERES). JPSS-1 instruments will provide satellite imagery, products and applications to users. The applications include detecting water and ice clouds, snow, sea surface temperatures, fog, fire, severe weather, vegetation health, aerosols, and sensing reflected lunar and emitted visible-wavelength light during the nighttime via the Day/Night Band (DNB) sensor included on VIIRS. Currently, there are only a few polar products that are operational for forecasters, however, more products will become available in the near future via Advanced Weather Interactive Processing System-II (AWIPS-II)-a forecasting analysis software package that forecasters can use to analyze meteorological data. To complement the polar products an wealth of training materials are currently in development. Denoted as the Satellite Foundational Course for JPSS (SatFC-J), this training will benefit NWS forecasters to utilize satellite data in their forecasts and daily operations as they discover their operational value in the NWS forecast process. As JPSS-1 launch nears, training materials will be produced in the form of modules, videos, quick guides, fact sheets, and hands-on exercises.

NEWTON - NEW portable multi-sensor scienTific instrument for non-invasive ON-site characterization of rock from planetary surface and sub-surfaces

NASA Astrophysics Data System (ADS)

Díaz-Michelena, M.; de Frutos, J.; Ordóñez, A. A.; Rivero, M. A.; Mesa, J. L.; González, L.; Lavín, C.; Aroca, C.; Sanz, M.; Maicas, M.; Prieto, J. L.; Cobos, P.; Pérez, M.; Kilian, R.; Baeza, O.; Langlais, B.; Thébault, E.; Grösser, J.; Pappusch, M.

2017-09-01

In space instrumentation, there is currently no instrument dedicated to susceptibly or complete magnetization measurements of rocks. Magnetic field instrument suites are generally vector (or scalar) magnetometers, which locally measure the magnetic field. When mounted on board rovers, the electromagnetic perturbations associated with motors and other elements make it difficult to reap the benefits from the inclusion of such instruments. However, magnetic characterization is essential to understand key aspects of the present and past history of planetary objects. The work presented here overcomes the limitations currently existing in space instrumentation by developing a new portable and compact multi-sensor instrument for ground breaking high-resolution magnetic characterization of planetary surfaces and sub-surfaces. This new technology introduces for the first time magnetic susceptometry (real and imaginary parts) as a complement to existing compact vector magnetometers for planetary exploration. This work aims to solve the limitations currently existing in space instrumentation by means of providing a new portable and compact multi-sensor instrument for use in space, science and planetary exploration to solve some of the open questions on the crustal and more generally planetary evolution within the Solar System.

AstroMadrid: Astrophysics and technological developments in Comunidad de Madrid

NASA Astrophysics Data System (ADS)

Mas-Hesse, J. M.

2011-11-01

AstroMadrid is a network constituted by different research groups in the Comunidad de Madrid area, with the objective of coordinating the activities related to the development of astronomical instrumentation in the various centres. AstroMadrid is a multidisciplinar team which benefits from the synergies provided by the different participating groups, optimizing our capabilities to develop instrumentation, and minimizing the problems related to the geographical dispersion within our region. AstroMadrid is also participated by several aerospace industries, which complement the capabilities and facilities available in the research centres. In addition to optimizing the development of instrumentation, AstroMadrid plays an essential role in the formation of new engineers and scientists, by actively contributing to some Master degree courses organized by different Universities in Madrid.

THE PATHOPHYSIOLOGY OF GEOGRAPHIC ATROPHY SECONDARY TO AGE-RELATED MACULAR DEGENERATION AND THE COMPLEMENT PATHWAY AS A THERAPEUTIC TARGET

PubMed Central

Schmidt-Erfurth, Ursula; van Lookeren Campagne, Menno; Henry, Erin C.; Brittain, Christopher

2017-01-01

Purpose: Geographic atrophy (GA) is an advanced, vision-threatening form of age-related macular degeneration (AMD) affecting approximately five million individuals worldwide. To date, there are no approved therapeutics for GA treatment; however, several are in clinical trials. This review focuses on the pathophysiology of GA, particularly the role of complement cascade dysregulation and emerging therapies targeting the complement cascade. Methods: Primary literature search on PubMed for GA, complement cascade in age-related macular degeneration. ClinicalTrials.gov was searched for natural history studies in GA and clinical trials of drugs targeting the complement cascade for GA. Results: Cumulative damage to the retina by aging, environmental stress, and other factors triggers inflammation via multiple pathways, including the complement cascade. When regulatory components in these pathways are compromised, as with several GA-linked genetic risk factors in the complement cascade, chronic inflammation can ultimately lead to the retinal cell death characteristic of GA. Complement inhibition has been identified as a key candidate for therapeutic intervention, and drugs targeting the complement pathway are currently in clinical trials. Conclusion: The complement cascade is a strategic target for GA therapy. Further research, including on natural history and genetics, is crucial to expand the understanding of GA pathophysiology and identify effective therapeutic targets. PMID:27902638

GOSAT validation out standing in the field: A case study of satellite validation using the SSEC Portable Atmospheric Research Center (SPARC)

NASA Astrophysics Data System (ADS)

Wagner, T. J.; Borg, L. A.; Feltz, M.; Gero, P. J.; Knuteson, R. O.; Olson, E.

2016-12-01

The Space Science and Engineering Center (SSEC) at the University of Wisconsin-Madison has developed the SSEC Portable Atmospheric Research Center (SPARC), a mobile 11 m trailer that houses numerous in situ and ground-based remote sensing instruments. Available instrumentation includes the Atmospheric Emitted Radiance Interferometer (AERI), a hyperspectral infrared radiometer from which trace gas concentrations and profiles of temperature and water vapor can be retrieved; the High Spectral Resolution Lidar (HSRL), a multichannel lidar capable of directly retrieving profiles of optical depth and backscatter depolarization; and a Doppler lidar wind profiler. The remote instrumentation suite is complemented by surface meteorology observations and a radiosonde ground station. Collectively, these instruments enable SPARC to participate in a wide variety of field studies, including meteorological field experiments and ground-based satellite calibration and validation studies. In August 2016, SPARC traveled to the Chequamegon National Forest in northern Wisconsin for a two week long deployment alongside the WLEF-TV tower. This 447 m tower houses long-term observations of thermodynamic and atmospheric composition at multiple heights, enabling studies of phenomena like atmospheric/land surface interactions and carbon uptake. During this deployment, SPARC launched radiosondes coincident with clear-sky overpasses of the Greenhouse gases Observing SATellite (GOSAT). Thermodynamic profiles from the radiosondes and AERI combined with the trace gas observations from the tower were used to validate the GOSAT observations of carbon dioxide and methane. The on-site presence of SPARC allowed for better characterization of the environment and greater observational certainty than was possible with the tower alone. Examples from this particular validation study as well as a discussion of how SPARC can contribute to other satellite calibration and validation investigations will be presented.

Use of satellite data in volcano monitoring

NASA Technical Reports Server (NTRS)

Mcclelland, Lindsay

1987-01-01

It is argued that Total Ozone Mapping Spectrometer (TOMS) data, especially data on sulfur dioxide detection in volcanic clouds, and weather satellite data complement each other. TOMS data is most useful for discovering previously unknown eruptions and indicating a minimum volume of SO sub 2 produced by a given eruption. Once an eruption has been reported, weather satellite data can be used to accurately monitor its progress. To be used effectively, these data need to be analyzed jointly and in real time. Toward this end, it is hoped that full and timely utilization can be made of existing TOMS data, a polar orbiting TOMS can be launched in the near future, and that TOMS type instruments can be included on future geostationary satellites.

SOFIA: A Promising Resource for Future Nova Studies

NASA Astrophysics Data System (ADS)

Helton, L. A.; Sofia Science Team

2014-12-01

The Stratospheric Observatory for Infrared Astronomy (SOFIA) is a 2.5-m telescope carried on board a Boeing 747-SP aircraft. Optimized for observations from infrared through sub-mm wavelengths, SOFIA observes from an altitude of 37,000 - 45,000 feet, above 99% of the atmospheric water vapor. The Observatory's complement of instruments possesses a broad range of capabilities, many of which are especially well suited for observations of classical novae, recurrent novae, and other cataclysmic variables. Here we present a selection of the instruments available on board SOFIA that may prove to be very useful for future novae studies.

Stratospheric Observatory for Infrared Astronomy (SOFIA)

NASA Astrophysics Data System (ADS)

Becklin, Eric E.; Horn, Jochen M. M.

The joint US and German SOFIA project to develop and operate a 2.5 - meter infrared airborne telescope in a Boeing 747-SP is now well into development. Work on the aircraft and the telescope has started. First science flights will begin in 2003 with 20% of the observing time assigned to German investigators. The observatory is expected to operate for over 20 years. The sensitivity, characteristics and science instrument complement are discussed. SOFIA will have instrumentation that will allow astronomical surveys that were not possible on the KAO. A future SOFIA project related to astrochemistry is discussed.

Stratospheric Observatory For Infrared Astronomy (SOFIA)

NASA Astrophysics Data System (ADS)

Becklin, E. E.; Davidson, J. A.; Horn, J. M. M.

1999-08-01

The joint US and German SOFIA project to develop and operate a 2.5 - meter infrared airborne telescope in a Boeing 747-SP is now in its second year of development. Work on the aircraft and the primary mirror has started. First science flights will begin in 2002 with 20% of the observing time assigned to German investigators. The observatory is expected to operate for over 20 years. The sensitivity, characteristics and science instrument complement are discussed. SOFIA will have facility instrumentation that will allow astronomical surveys that were not possible on the KAO. Two future SOFIA projects related to cosmology and astrochemistry are discussed.

The Influence of the Use of Technology on Student Outcomes in a Blended Learning Context

ERIC Educational Resources Information Center

López-Pérez, María V.; Pérez-López, María C.; Rodríguez-Ariza, Lázaro; Argente-Linares, Eva

2013-01-01

In blended learning, the internet acts as an instrument to complement traditional forms of instruction, in the belief that the incorporation of new information and communication technologies may lead to more efficient and effective education. This paper presents a study carried out in the University of Granada, during the first year of…

Dynamical computation of constrained flexible systems using a modal Udwadia-Kalaba formulation: Application to musical instruments.

PubMed

Antunes, J; Debut, V

2017-02-01

Most musical instruments consist of dynamical subsystems connected at a number of constraining points through which energy flows. For physical sound synthesis, one important difficulty deals with enforcing these coupling constraints. While standard techniques include the use of Lagrange multipliers or penalty methods, in this paper, a different approach is explored, the Udwadia-Kalaba (U-K) formulation, which is rooted on analytical dynamics but avoids the use of Lagrange multipliers. This general and elegant formulation has been nearly exclusively used for conceptual systems of discrete masses or articulated rigid bodies, namely, in robotics. However its natural extension to deal with continuous flexible systems is surprisingly absent from the literature. Here, such a modeling strategy is developed and the potential of combining the U-K equation for constrained systems with the modal description is shown, in particular, to simulate musical instruments. Objectives are twofold: (1) Develop the U-K equation for constrained flexible systems with subsystems modelled through unconstrained modes; and (2) apply this framework to compute string/body coupled dynamics. This example complements previous work [Debut, Antunes, Marques, and Carvalho, Appl. Acoust. 108, 3-18 (2016)] on guitar modeling using penalty methods. Simulations show that the proposed technique provides similar results with a significant improvement in computational efficiency.

Updates on the Performance and Calibration of HST/STIS

NASA Astrophysics Data System (ADS)

Lockwood, Sean A.; Monroe, TalaWanda R.; Ogaz, Sara; Branton, Doug; Carlberg, Joleen K.; Debes, John H.; Jedrzejewski, Robert I.; Proffitt, Charles R.; Riley, Allyssa; Sohn, Sangmo Tony; Sonnentrucker, Paule; Walborn, Nolan R.; Welty, Daniel

2018-06-01

The Space Telescope Imaging Spectrograph (STIS) on the Hubble Space Telescope (HST) has been in orbit for 21 years and continues to produce high quality scientific results using a diverse complement of operating modes. These include spatially resolved spectroscopy in the UV and optical, high spatial resolution echelle spectroscopy in the UV, and solar-blind imaging in the UV. In addition, STIS possesses unique visible-light coronagraphic modes that keep the instrument at the forefront of exoplanet and debris-disk research. As the instrument's characteristics evolve over its lifetime, the instrument team at the Space Telescope Science Institute monitors its performance and works towards improving the quality of its data products. Here we present updates on the status of the STIS CCD and FUV & NUV MAMA detectors, as well as changes to the CalSTIS reduction pipeline. We also discuss progress toward the recalibration of the E140M/1425 echelle mode. The E140M grating blaze function shapes have changed since flux calibration was carried out following SM4, which limits the relative photometric flux accuracy of some spectral orders up to 5-10% at the edges. In Cycle 25 a special calibration program was executed to obtain updated sensitivity curves for the E140M/1425 setting.

Results from Multiwavelength Workshop for Next Generation Gamma Ray Experiments

NASA Astrophysics Data System (ADS)

Fortson, L.

2002-12-01

The next few years will see the build up of several new gamma-ray detectors both on the ground and in space. By 2006 VERITAS, HESS and MAGIC expect to be operational and GLAST will be in orbit. At the same time, a number of X-ray satellites will be in operation, complementing these new gamma-ray instruments. A better understanding of many high-energy sources can be obtained by making contemporaneous observations with multiple x-ray and gamma-ray instruments. A workshop was recently held at the Adler Planetarium and Astronomy Museum in Chicago to discuss the future of multiwavelength campaigns. The workshop was intended as an opportunity for information exchange within the community to get the best possible science returns from the wealth of data that is expected to come in from the next generation of experiments. By the end of the workshop participants gained a general understanding of the capabilities of the various instruments and their observational strategies. We also came up with a good start on some concrete mechanisms for coordinating gamma-ray observations with ground based and space based observatories at other wavelengths - including X-ray and optical groups. I will report on the results from this workshop in my presentation at the AAS. The workshop was sponsored by the Adler Planetarium and Astronomy Museum.

Overview of Space Station attached payloads in the areas of solar physics, solar terrestrial physics, and plasma processes

NASA Technical Reports Server (NTRS)

Roberts, W. T.; Kropp, J.; Taylor, W. W. L.

1986-01-01

This paper outlines the currently planned utilization of the Space Station to perform investigations in solar physics, solar terrestrial physics, and plasma physics. The investigations and instrumentation planned for the Solar Terrestrial Observatory (STO) and its associated Space Station accommodation requirements are discussed as well as the planned placement of the STO instruments and typical operational scenarios. In the area of plasma physics, some preliminary plans for scientific investigations and for the accommodation of a plasma physics facility attached to the Space Station are outlined. These preliminary experiment concepts use the space environment around the Space Station as an unconfined plasma laboratory. In solar physics, the initial instrument complement and associated accommodation requirements of the Advanced Solar Observatory are described. The planned evolutionary development of this observatory is outlined, making use of the Space Station capabilities for servicing and instrument reconfiguration.

Cryo-vacuum testing of the JWST Integrated Science Instrument Module (SPIE)

NASA Technical Reports Server (NTRS)

Kimble, Randy A.; Vila, M. Begona; Van Campen, Julie; Birkmann, Stephan M.; Comber, Brian J.; Fatig, Curtis C.; Glasse, Alistair C. H.; Glazer, Stuart D.; Kelly, Douglas M.; Mann, Steven D.;

Innovative market-based policy instruments for waste management: A case study on shredder residues in Belgium.

PubMed

Dubois, Maarten; Hoogmartens, Rob; Van Passel, Steven; Van Acker, Karel; Vanderreydt, Ive

2015-10-01

In an increasingly complex waste market, market-based policy instruments, such as disposal taxes, can give incentives for sustainable progress while leaving flexibility for innovation. However, implementation of disposal taxes is often criticised by domestic waste handlers that fear to be outcompeted by competitors in other countries. The article discusses three innovative market-based instruments that limit the impact on international competitiveness: Tradable recycling credits, refunded disposal taxes and differentiated disposal taxes. All three instruments have already been implemented for distinct environmental policies in Europe. In order to illustrate how these instruments can be used for waste policy, the literature review is complemented with a case study on shredder residues from metal-containing waste streams in Belgium. The analysis shows that a conventional disposal tax remains the most efficient, simple and transparent instrument. However, if international competition is a significant issue or if political support is weak, refunded and differentiated disposal taxes can have an added value as second-best instruments. Tradable recycling credits are not an appropriate instrument for use in small waste markets with market power. In addition, refunded taxes create similar incentives, but induce lower transactions costs. © The Author(s) 2015.

HARMONI instrument control electronics

NASA Astrophysics Data System (ADS)

Gigante, José V.; Rodríguez Ramos, Luis F.; Zins, Gerard; Schnetler, Hermine; Pecontal, Arlette; Herreros, José Miguel; Clarke, Fraser; Bryson, Ian; Thatte, Niranjan

2014-07-01

HARMONI is an integral field spectrograph working at visible and near-infrared wavelengths over a range of spatial scales from ground layer corrected to fully diffraction-limited. The instrument has been chosen to be part of the first-light complement at the European Extremely Large Telescope (E-ELT). This paper describes the instrument control electronics to be developed at IAC. The large size of the HARMONI instrument, its cryogenic operation, and the fact that it must operate with enhanced reliability is a challenge from the point of view of the control electronics design. The present paper describes a design proposal based on the current instrument requirements and intended to be fully compliant with the ESO E-ELT standards, as well as with the European EMC and safety standards. The modularity of the design and the use of COTS standard hardware will benefit the project in several aspects, as reduced costs, shorter schedule by the use of commercially available components, and improved quality by the use of well proven solutions.

The UV Survey Mission Concept, CETUS

NASA Astrophysics Data System (ADS)

Heap, Sara; and the CETUS Team

2018-01-01

In March 2017, NASA selected CETUS for study of a Probe-class mission concept. W. Danchi is the CETUS PI, and S. Heap is the Science PI. CETUS is primarily a UV survey telescope to complement survey telescopes of the 2020’s including E-ROSITA, Subaru Hyper Suprime Cam and Prime-Focus Spectrograph, WFIRST, and the Square Kilometer Array. CETUS comprises a 1.5-m wide-field telescope and three science instruments: a wide-field (1045” on a side) far-UV and near-UV camera; a similarly wide-field near-UV multi-object spectrograph utilizing a next-generation micro-shutter array; and a single-object spectrograph with options of spectral region (far-UV or near-UV) and spectral resolving power (2,000 or 40,000). The survey instruments will operate simultaneously thereby producing wide-field images in the near-UV and far-UV and a spectrogram containing near-UV spectra of up to 100 sources free of spectral overlap and astronomical background. ln concert with other survey telescopes, CETUS will focus on understanding galaxy evolution at cosmic noon (z~1-2).

Observations of Exoplanets with the Stratospheric Observatory for Infrared Astronomy (SOFIA)

NASA Astrophysics Data System (ADS)

Gehrz, R.; Becklin, E.

2010-10-01

The joint U.S. and German Stratospheric Observatory for Infrared Astronomy (SOFIA) is a 2.5-meter infrared airborne telescope in a Boeing 747-SP that will begin science flights in 2010. Flying in the stratosphere at altitudes as high as 45,000 feet, SOFIA will be used to conduct photometric, spectroscopic, and imaging observations at wavelengths from 0.3 microns to 1.9 millimeters with an average transmission of greater than 80 percent. SOFIA's first-generation instrument complement includes high speed photometers, broadband imagers, moderate resolution spectrographs capable of resolving broad features due to dust and large molecules, and high resolution spectrometers suitable for kinematic studies of molecular and atomic gas lines at km/s resolution. These and future instruments will give SOFIA the potential to make unique contributions to the characterization of the atmospheres of exoplanets that transit their parent stars. First-light images obtained on May 26, 2010 with the FORCAST imager will be shown. We will discuss several types of experiments that are being contemplated with respect to observations of exoplanets .

Assertiveness expectancies: how hard people push depends on the consequences they predict.

PubMed

Ames, Daniel R

2008-12-01

The present article seeks to explain varying levels of assertiveness in interpersonal conflict and negotiations with assertiveness expectancies, idiosyncratic predictions people make about the social and instrumental consequences of assertive behavior. This account complements motivation-based models of assertiveness and competitiveness, suggesting that individuals may possess the same social values (e.g., concern for relationships) but show dramatically different assertiveness due to different assumptions about behavioral consequences. Results clarify the form of assertiveness expectancies, namely that most people assume increasing assertiveness can yield positive social and instrumental benefits up to a point, beyond which benefits decline. However, people vary in how assertive this perceived optimal point is. These individual differences in expectancies are linked in 4 studies to assertiveness, including self-reported assertiveness, rated behavioral preferences in assorted interpersonal conflict scenarios, partner ratings of participants' behavior in a face-to-face dyadic negotiation, and work colleague ratings of participants' assertiveness in the workplace. In each case, the link between expectancies and behavior remained after controlling for values. The results suggest a place for expectancies alongside values in psychological models of interpersonal assertiveness.

Towards Automating Clinical Assessments: A Survey of the Timed Up and Go (TUG)

PubMed Central

Sprint, Gina; Cook, Diane; Weeks, Douglas

2016-01-01

Older adults often suffer from functional impairments that affect their ability to perform everyday tasks. To detect the onset and changes in abilities, healthcare professionals administer standardized assessments. Recently, technology has been utilized to complement these clinical assessments to gain a more objective and detailed view of functionality. In the clinic and at home, technology is able to provide more information about patient performance and reduce subjectivity in outcome measures. The timed up and go (TUG) test is one such assessment recently instrumented with technology in several studies, yielding promising results towards the future of automating clinical assessments. Potential benefits of technological TUG implementations include additional performance parameters, generated reports, and the ability to be self-administered in the home. In this paper, we provide an overview of the TUG test and technologies utilized for TUG instrumentation. We then critically review the technological advancements and follow up with an evaluation of the benefits and limitations of each approach. Finally, we analyze the gaps in the implementations and discuss challenges for future research towards automated, self-administered assessment in the home. PMID:25594979

The Heavy Nuclei eXplorer (HNX) Small Explorer Mission

NASA Astrophysics Data System (ADS)

Mitchell, John; Binns, W. Robert; Hams, Thomas; Israel, Martin; Krizmanic, John; Link, Jason; Rauch, Brian; Sakai, Kenichi; Sasaki, Makoto; Westphal, Andrew; Wiedenbeck, Mark; Heavy Nuclei eXplorer Collaboration

2015-04-01

The Heavy Nuclei eXplorer (HNX) will investigate the nature of the reservoirs of nuclei at the cosmic-ray sources, the mechanisms by which nuclei are removed from the reservoirs and injected into the cosmic accelerators, and the acceleration mechanism. HNX will use two large high-precision instruments, the Extremely-heavy Cosmic-ray Composition Observer (ECCO) and the Cosmic-ray Trans-Iron Galactic Element Recorder (CosmicTIGER), flying in the SpaceX DragonLab, to measure, for the first time, the abundance of every individual element in the periodic table from carbon through the actinides, providing the first measurement of many of these elements. HNX will measure several thousand ultra-heavy galactic cosmic ray (UHGCR) nuclei Z >= 30, including about 50 actinides, and will: determine whether GCRs are accelerated from new or old material, and find their age; measure the mix of nucleosynthesis processes responsible for the UHGCRs; determine how UHGCR elements are selected for acceleration, and measure the mean integrated pathlength traversed by UHGCRs before observation. The scientific motivation and instrument complement of HNX will be discussed.

Environmental monitors in the Midcourse Space Experiments (MSX)

NASA Technical Reports Server (NTRS)

Uy, O. M.

1993-01-01

The Midcourse Space Experiment (MSX) is an SDIO sponsored space based sensor experiment with a full complement of optical sensors. Because of the possible deleterious effect of both molecular and particulate contamination on these sensors, a suite of environmental monitoring instruments are also being flown with the spacecraft. These instruments are the Total Pressure Sensor based on the cold-cathode gauge, a quadrupole mass spectrometer, a Bennett-type ion mass spectrometer, a cryogenic quartz crystal microbalance (QCM), four temperature-controlled QCM's, and a Xenon and Krypton Flash Lamp Experiment. These instruments have been fully space-qualified, are compact and low cost, and are possible candidate sensors for near-term planetary and atmospheric monitoring. The philosophy adopted during design and fabrication, calibration and ground testing, and modeling will be discussed .

Republic P-47G Thunderbolt and the NACA Flight Operations Crew

NASA Image and Video Library

1944-03-21

The Flight Operations crew stands before a Republic P-47G Thunderbolt at the National Advisory Committee for Aeronautics (NACA) Aircraft Engine Research Laboratory in Cleveland, Ohio. The laboratory’s Flight Research Section was responsible for conducting a variety of research flights. During World War II most of the test flights complemented the efforts in ground-based facilities to improve engine cooling systems or study advanced fuel mixtures. The Republic P–47G was loaned to the laboratory to test NACA modifications to the Wright R–2800 engine’s cooling system at higher altitudes. The laboratory has always maintained a fleet of aircraft so different research projects were often conducted concurrently. The flight research program requires an entire section of personnel to accomplish its work. This staff generally consists of a flight operations group, which includes the section chief, pilots and administrative staff; a flight maintenance group with technicians and mechanics responsible for inspecting aircraft, performing checkouts and installing and removing flight instruments; and a flight research group that integrates the researchers’ experiments into the aircraft. The staff at the time of this March 1944 photograph included 3 pilots, 16 planning and analysis engineers, 36 mechanics and technicians, 10 instrumentation specialists, 6 secretaries and 5 computers.

GOES-R L1b Readiness Implementation and Management Plan

NASA Technical Reports Server (NTRS)

Kunkee, David; Farley, Robert; Kwan, Betty; Walterscheid, Richard; Hecht, James; Claudepierre, Seth.; De Luccia, Frank

2017-01-01

A complement of Readiness, Implementation and Management Plans (RIMPs) to facilitate management of post-launch product test activities for the official Geostationary Operational Environmental Satellite (GOES-R) Level 1b (L1b) products have been developed and documented. Separate plans have been created for each of the GOES-R sensors including: the Advanced Baseline Imager (ABI), the Extreme ultraviolet and X-ray Irradiance Sensors (EXIS), Geostationary Lightning Mapper (GLM), GOES-R Magnetometer (MAG), the Space Environment In-Situ Suite (SEISS), and the Solar Ultraviolet Imager (SUVI). The GOES-R program has implemented these RIMPs in order to address the full scope of CalVal activities required for a successful demonstration of GOES-R L1b data product quality throughout the three validation stages: Beta, Provisional and Full Validation. For each product maturity level, the RIMPs include specific performance criteria and required artifacts that provide evidence a given validation stage has been reached, the timing when each stage will be complete, a description of every applicable Post-Launch Product Test (PLPT), roles and responsibilities of personnel, upstream dependencies, and analysis methods and tools to be employed during validation. Instrument level Post-Launch Tests (PLTs) are also referenced and apply primarily to functional check-out of the instruments.

Development of Level 1b Calibration and Validation Readiness, Implementation and Management Plans for GOES-R

NASA Technical Reports Server (NTRS)

Kunkee, David B.; Farley, Robert W.; Kwan, Betty P.; Hecht, James H.; Walterscheid, Richard L.; Claudepierre, Seth G.; Bishop, Rebecca L.; Gelinas, Lynette J.; Deluccia, Frank J.

2017-01-01

A complement of Readiness, Implementation and Management Plans (RIMPs) to facilitate management of post-launch product test activities for the official Geostationary Operational Environmental Satellite (GOES-R) Level 1b (L1b) products have been developed and documented. Separate plans have been created for each of the GOES-R sensors including: the Advanced Baseline Imager (ABI), the Extreme ultraviolet and X-ray Irradiance Sensors (EXIS), Geostationary Lightning Mapper (GLM), GOES-R Magnetometer (MAG), the Space Environment In-Situ Suite (SEISS), and the Solar Ultraviolet Imager (SUVI). The GOES-R program has implemented these RIMPs in order to address the full scope of CalVal activities required for a successful demonstration of GOES-R L1b data product quality throughout the three validation stages: Beta, Provisional and Full Validation. For each product maturity level, the RIMPs include specific performance criteria and required artifacts that provide evidence a given validation stage has been reached, the timing when each stage will be complete, a description of every applicable Post-Launch Product Test (PLPT), roles and responsibilities of personnel, upstream dependencies, and analysis methods and tools to be employed during validation. Instrument level Post-Launch Tests (PLTs) are also referenced and apply primarily to functional check-out of the instruments.

Propulsive Maneuver Design for the 2007 Mars Phoenix Lander Mission

NASA Technical Reports Server (NTRS)

Raofi, Behzad; Bhat, Ramachandra S.; Helfrich, Cliff

2008-01-01

On May 25, 2008, the Mars Phoenix Lander (PHX) successfully landed in the northern planes of Mars in order to continue and complement NASA's "follow the water" theme as its predecessor Mars missions, such as Mars Odyssey (ODY) and Mars Exploration Rovers, have done in recent years. Instruments on the lander, through a robotic arm able to deliver soil samples to the deck, will perform in-situ and remote-sensing investigations to characterize the chemistry of materials at the local surface, subsurface, and atmosphere. Lander instruments will also identify the potential history of key indicator elements of significance to the biological potential of Mars, including potential organics within any accessible water ice. Precise trajectory control and targeting were necessary in order to achieve the accurate atmospheric entry conditions required for arriving at the desired landing site. The challenge for the trajectory control maneuver design was to meet or exceed these requirements in the presence of spacecraft limitations as well as other mission constraints. This paper describes the strategies used, including the specialized targeting specifically developed for PHX, in order to design and successfully execute the propulsive maneuvers that delivered the spacecraft to its targeted landing site while satisfying the planetary protection requirements in the presence of flight system constraints.

Stratospheric Observatory for Infrared Astronomy (SOFIA)

NASA Astrophysics Data System (ADS)

Becklin, E. E.

The joint US and German SOFIA project to develop and operate a 2.5 m infrared airborne telescope in a Boeing 747-SP is now in the final stages of development. First science flights will begin in 2007. The observatory is expected to operate for over 20 years. The sensitivity, characteristics, science instrument complement, and examples of first light science are discussed.

The TOPEX satellite option study

NASA Technical Reports Server (NTRS)

1982-01-01

The applicability of an existing spacecraft bus and subsystems to the requirements of ocean circulation measurements are assessed. The operational meteorological satellite family TIROS and DMSP are recommended. These programs utilize a common bus to satisfy their Earth observation missions. Note that although the instrument complements were different, the pointing accuracies were different, and, initially, the boosters were different, a high degree of commonality was achieved.

Complement Activation in Inflammatory Skin Diseases

PubMed Central

Giang, Jenny; Seelen, Marc A. J.; van Doorn, Martijn B. A.; Rissmann, Robert; Prens, Errol P.; Damman, Jeffrey

2018-01-01

The complement system is a fundamental part of the innate immune system, playing a crucial role in host defense against various pathogens, such as bacteria, viruses, and fungi. Activation of complement results in production of several molecules mediating chemotaxis, opsonization, and mast cell degranulation, which can contribute to the elimination of pathogenic organisms and inflammation. Furthermore, the complement system also has regulating properties in inflammatory and immune responses. Complement activity in diseases is rather complex and may involve both aberrant expression of complement and genetic deficiencies of complement components or regulators. The skin represents an active immune organ with complex interactions between cellular components and various mediators. Complement involvement has been associated with several skin diseases, such as psoriasis, lupus erythematosus, cutaneous vasculitis, urticaria, and bullous dermatoses. Several triggers including auto-antibodies and micro-organisms can activate complement, while on the other hand complement deficiencies can contribute to impaired immune complex clearance, leading to disease. This review provides an overview of the role of complement in inflammatory skin diseases and discusses complement factors as potential new targets for therapeutic intervention. PMID:29713318

The Pioneer XI high field fluxgate magnetometer

NASA Technical Reports Server (NTRS)

Acuna, M. A.; Ness, N. F.

1975-01-01

The high field fluxgate magnetometer experiment flown aboard the Pioneer XI spacecraft is described. This extremely simple instrument was used to extend the spacecraft's upper-limit measurement capability by approximately an order of magnitude (from 0.14 mT to 1.00 mT) with minimum power and volume requirements. This magnetometer was designed to complement the low-field measurements provided by a helium vector magnetometer and utilizes magnetic ring core sensors with biaxial orthogonal sense coils. The instrument is a single-range, triaxial-fluxgate magnetometer capable of measuring fields of up to 1 mT along each orthogonal axis, with a maximum resolution of 1 microT.

The Complement System in Dialysis: A Forgotten Story?

PubMed Central

Poppelaars, Felix; Faria, Bernardo; Gaya da Costa, Mariana; Franssen, Casper F. M.; van Son, Willem J.; Berger, Stefan P.; Daha, Mohamed R.; Seelen, Marc A.

2018-01-01

Significant advances have lead to a greater understanding of the role of the complement system within nephrology. The success of the first clinically approved complement inhibitor has created renewed appreciation of complement-targeting therapeutics. Several clinical trials are currently underway to evaluate the therapeutic potential of complement inhibition in renal diseases and kidney transplantation. Although, complement has been known to be activated during dialysis for over four decades, this area of research has been neglected in recent years. Despite significant progress in biocompatibility of hemodialysis (HD) membranes and peritoneal dialysis (PD) fluids, complement activation remains an undesired effect and relevant issue. Short-term effects of complement activation include promoting inflammation and coagulation. In addition, long-term complications of dialysis, such as infection, fibrosis and cardiovascular events, are linked to the complement system. These results suggest that interventions targeting the complement system in dialysis could improve biocompatibility, dialysis efficacy, and long-term outcome. Combined with the clinical availability to safely target complement in patients, the question is not if we should inhibit complement in dialysis, but when and how. The purpose of this review is to summarize previous findings and provide a comprehensive overview of the role of the complement system in both HD and PD. PMID:29422906

Initial Results from CALIPSO

NASA Technical Reports Server (NTRS)

Winker, David M.; Pelon, Jacques; McCormick, M. Patrick

2006-01-01

CALIPSO will carry the first polarization lidar in orbit, along with infrared and visible passive imagers, and will fly in formation as part of the Afternoon Constellation (A-train). The acquisition of observations which are simultaneous and coincident with observations from other instruments of the A-train will allow numerous synergies to be realized from combining CALIPSO observations with observations from other platforms. In particular, cloud observations from the CALIPSO lidar and the CloudSat radar will complement each other, together encompassing the variety of clouds found in the atmosphere, from thin cirrus to deep convective clouds. CALIPSO has been developed within the framework of a collaboration between NASA and CNES and is currently scheduled to launch, along with the CloudSat satellite, in spring 2006. This paper will present an overview of the CALIPSO mission, including initial results.

Functional, Structural, and Neurotoxicity Biomarkers in Integrative Assessment of Concussions

PubMed Central

Dambinova, Svetlana A.; Maroon, Joseph C.; Sufrinko, Alicia M.; Mullins, John David; Alexandrova, Eugenia V.; Potapov, Alexander A.

2016-01-01

Concussion is a complex, heterogeneous process affecting the brain. Accurate assessment and diagnosis and appropriate management of concussion are essential to ensure that athletes do not prematurely return to play or others to work or active military duty, risking re-injury. To date, clinical diagnosis relies primarily on evaluating subjects for functional impairment using instruments that include neurocognitive testing, subjective symptom report, and neurobehavioral assessments, such as balance and vestibular-ocular reflex testing. Structural biomarkers, defined as advanced neuroimaging techniques and biomarkers assessing neurotoxicity and immunoexcitotoxicity, may complement the use of functional biomarkers. We hypothesize that neurotoxicity AMPA, NMDA, and kainite receptor biomarkers might be utilized as a part of comprehensive approach to concussion evaluations, with the goal of increasing diagnostic accuracy and facilitating treatment planning and prognostic assessment. PMID:27761129

The Potassium-Argon Laser Experiment (KARLE): In Situ Geochronology for Planetary Robotic Missions

NASA Technical Reports Server (NTRS)

Cohen, B. A.; Devismes, D.; Miller, J. S.; Swindle, T. D.

2014-01-01

Isotopic dating is an essential tool to establish an absolute chronology for geological events, including crystallization history, magmatic evolution, and alteration events. The capability for in situ geochronology will open up the ability for geochronology to be accomplished as part of lander or rover complement, on multiple samples rather than just those returned. An in situ geochronology package can also complement sample return missions by identifying the most interesting rocks to cache or return to Earth. The K-Ar Laser Experiment (KArLE) brings together a novel combination of several flight-proven components to provide precise measurements of potassium (K) and argon (Ar) that will enable accurate isochron dating of planetary rocks. KArLE will ablate a rock sample, measure the K in the plasma state using laser-induced breakdown spectroscopy (LIBS), measure the liberated Ar using mass spectrometry (MS), and relate the two by measuring the volume of the ablated pit by optical imaging. Our work indicates that the KArLE instrument is capable of determining the age of planetary samples with sufficient accuracy to address a wide range of geochronology problems in planetary science. Additional benefits derive from the fact that each KArLE component achieves analyses useful for most planetary surface missions.

Generation of Viable Mice from Induced Pluripotent Stem Cells (iPSCs) Through Tetraploid Complementation.

PubMed

Kang, Lan; Gao, Shaorong

2015-01-01

Tetraploid complementation assay is the most rigorous criteria for pluripotency characterization of pluripotent stem cells including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). Pluripotent stem cells could complement the developmental deficiency of tetraploid embryos and thus support the full-term mice development. Here we describe the protocol for tetraploid complementation using iPSCs to produce viable all-iPSC mice.

Breaking down the complement system: a review and update on novel therapies.

PubMed

Reddy, Yuvaram N V; Siedlecki, Andrew M; Francis, Jean M

2017-03-01

The complement system represents one of the more primitive forms of innate immunity. It has increasingly been found to contribute to pathologies in the native and transplanted kidney. We provide a concise review of the physiology of the complement cascade, and discuss current and upcoming complement-based therapies. Current agents in clinical use either bind to complement components directly or prevent complement from binding to antibodies affixed to the endothelial surface. These include C1 esterase inhibitors, anti-C5 mAbs, anti-CD20 mAbs, and proteasome inhibitors. Treatment continues to show efficacy in the atypical hemolytic uremic syndrome and antibody-mediated rejection. Promising agents not currently available include CCX168, TP10, AMY-101, factor D inhibitors, coversin, and compstatin. Several new trials are targeting complement inhibition to treat antineutrophilic cystoplasmic antibody (ANCA)-associated vasculitis, C3 glomerulopathy, thrombotic microangiopathy, and IgA nephropathy. New agents for the treatment of the atypical hemolytic uremic syndrome are also in development. Complement-based therapies are being considered for targeted therapy in the atypical hemolytic uremic syndrome and antibody-mediated rejection, C3 glomerulopathy, and ANCA-associated vasculitis. A few agents are currently in use as orphan drugs. A number of other drugs are in clinical trials and, overall, are showing promising preliminary results.

COMET: a planned airborne mission to simultaneously measure CO2 and CH4 columns using airborne remote sensing and in-situ techniques

NASA Astrophysics Data System (ADS)

Fix, A.; Amediek, A.; Büdenbender, C.; Ehret, G.; Wirth, M.; Quatrevalet, M.; Rapp, M.; Gerilowski, K.; Bovensmann, H.; Gerbig, C.; Pfeilsticker, K.; Zöger, M.; Giez, A.

2013-12-01

To better predict future trends in the cycles of the most important anthropogenic greenhouse gases, CO2 and CH4, there is a need to measure and understand their distribution and variation on various scales. To address these requirements it is envisaged to deploy a suite of state-of-the-art airborne instruments that will be capable to simultaneously measure the column averaged dry-air mixing ratios (XGHG) of both greenhouse gases along the flight path. As the measurement platform serves the research aircraft HALO, a modified Gulfstream G550, operated by DLR. This activity is dubbed CoMet (CO2 and Methane Mission). The instrument package of CoMet will consist of active and passive remote sensors as well as in-situ instruments to complement the column measurements by highly-resolved profile information. As an active remote sensing instrument CHARM-F, the integrated-path differential absorption lidar currently under development at DLR, will provide both, XCO2 and XCH4, below flight altitude. The lidar instrument will be complemented by MAMAP which is a NIR/SWIR absorption spectrometer developed by University of Bremen and which is also capable to derive XCH4 and XCO2. As an additional passive instrument, mini-DOAS operated by University of Heidelberg will contribute with additional context information about the investigated air masses. In order to compare the remote sensing instruments with integrated profile information, in-situ instrumentation is indispensable. The in-situ package will therefore comprise wavelength-scanned Cavity-Ring-Down Spectroscopy (CRDS) for the detection of CO2, CH4, CO and H2O and a flask sampler for collection of atmospheric samples and subsequent laboratory analysis. Furthermore, the BAsic HALO Measurement And Sensor System (BAHAMAS) will provide an accurate set of meteorological and aircraft state parameters for each scientific flight. Within the frame of the first CoMet mission scheduled for the 2015 timeframe it is planned to concentrate on small to sub-continental scale variations of the greenhouse gases. This does not only allow to identify local emission sources of GHGs, but also opens up the opportunity to use important remote sensing and in-situ data information for the inverse modeling approach for regional budgeting. CoMet is also targeting at providing a validation platform of future spaceborne GHG missions in particular the upcoming French-German methane mission MERLIN. CHARM-F was devised as an airborne demonstrator for MERLIN, and, as such will be a key instrument for MERLIN validation.

An instrument for broadened risk assessment in antenatal health care including non-medical issues

PubMed Central

Vos, Amber A.; van Veen, Mieke J.; Birnie, Erwin; Denktaş, Semiha; Steegers, Eric A.P.; Bonsel, Gouke J.

2015-01-01

Introduction Growing evidence on the risk contributing role of non-medical factors on pregnancy outcomes urged for a new approach in early antenatal risk selection. The evidence invites to more integration, in particular between the clinical working area and the public health domain. We developed a non-invasive, standardized instrument for comprehensive antenatal risk assessment. The current study presents the application-oriented development of a risk screening instrument for early antenatal detection of risk factors and tailored prevention in an integrated care setting. Methods A review of published instruments complemented with evidence from cohort studies. Selection and standardization of risk factors associated with small for gestational age, preterm birth, congenital anomalies and perinatal mortality. Risk factors were weighted to obtain a cumulative risk score. Responses were then connected to corresponding care pathways. A cumulative risk threshold was defined, which can be adapted to the population and the availability of preventive facilities. A score above the threshold implies multidisciplinary consultation between caregivers. Results The resulting digital score card consisted of 70 items, subdivided into four non-medical and two medical domains. Weighing of risk factors was based on existing evidence. Pilot-evidence from a cohort of 218 pregnancies in a multi-practice urban setting showed a cut-off of 16 points would imply 20% of all pregnant women to be assessed in a multidisciplinary setting. A total of 28 care pathways were defined. Conclusion The resulting score card is a universal risk screening instrument which incorporates recent evidence on non-medical risk factors for adverse pregnancy outcomes and enables systematic risk management in an integrated antenatal health care setting. PMID:25780351

Development of the Potassium-Argon Laser Experiment (KArLE) Instrument for In Situ Geochronology

NASA Technical Reports Server (NTRS)

Cohen, Barbara A.; Li, Z.-H.; Miller, J. S.; Brinckerhoff, W. B.; Clegg, S. M.; Mahaffy, P. R.; Swindle, T. D.; Wiens, R. C.

2012-01-01

Absolute dating of planetary samples is an essential tool to establish the chronology of geological events, including crystallization history, magmatic evolution, and alteration. Traditionally, geochronology has only been accomplishable on samples from dedicated sample return missions or meteorites. The capability for in situ geochronology is highly desired, because it will allow one-way planetary missions to perform dating of large numbers of samples. The success of an in situ geochronology package will not only yield data on absolute ages, but can also complement sample return missions by identifying the most interesting rocks to cache and/or return to Earth. In situ dating instruments have been proposed, but none have yet reached TRL 6 because the required high-resolution isotopic measurements are very challenging. Our team is now addressing this challenge by developing the Potassium (K) - Argon Laser Experiment (KArLE) under the NASA Planetary Instrument Definition and Development Program (PIDDP), building on previous work to develop a K-Ar in situ instrument [1]. KArLE uses a combination of several flight-proven components that enable accurate K-Ar isochron dating of planetary rocks. KArLE will ablate a rock sample, determine the K in the plasma state using laser-induced breakdown spectroscopy (LIBS), measure the liberated Ar using quadrupole mass spectrometry (QMS), and relate the two by the volume of the ablated pit using an optical method such as a vertical scanning interferometer (VSI). Our preliminary work indicates that the KArLE instrument will be capable of determining the age of several kinds of planetary samples to +/-100 Myr, sufficient to address a wide range of geochronology problems in planetary science.

An instrument for direct observations of seismic and normal-mode rotational oscillations of the Earth

PubMed Central

Cowsik, R.

2007-01-01

The rotations around the vertical axis associated with the normal mode oscillations of the Earth and those induced by the seismic and other disturbances have been very difficult to observe directly. Such observations will provide additional information for 3D modeling of the Earth and for understanding earthquakes and other underground explosions. In this paper, we describe the design of an instrument capable of measuring the rotational motions associated with the seismic oscillations of the Earth, including the lowest frequency normal mode at ν ≈ 3.7 × 10−4 Hz. The instrument consists of a torsion balance with a natural frequency of ν0 ≈ 1.6 × 10−4 Hz, which is observed by an autocollimating optical lever of high angular resolution and dynamic range. Thermal noise limits the sensitivity of the apparatus to amplitudes of ≈ 1.5 × 10−9 rad at the lowest frequency normal mode and the sensitivity improves as ν−3/2 with increasing frequency. Further improvements in sensitivity by about two orders of magnitude may be achieved by operating the balance at cryogenic temperatures. Alternatively, the instrument can be made more robust with a reduced sensitivity by increasing ν0 to ≈10−2 Hz. This instrument thus complements the ongoing effort by Igel and others to study rotational motions using ring laser gyroscopes and constitutes a positive response to the clarion call for developments in rotation seismology by Igel, Lee, and Todorovska [H. Igel, W.H.K. Lee and M.I. Todorovska, AGU Fall Meeting 2006, Rotational Seismology Sessions: S22A,S23B, Inauguration of the International Working Group on Rotational Seismology (IWGoRS)]. PMID:17438268

To score or not to score: a qualitative study on GPs views on the use of instruments for depression.

PubMed

Pettersson, Agneta; Björkelund, Cecilia; Petersson, Eva-Lisa

2014-04-01

To improve the detection, diagnosis and follow-up of depression in primary care patients, it has been proposed that GPs should employ assessment instruments as a complement to the consultation. However, most GPs do not use such instruments routinely. To explore perceptions of Swedish GPs on the use of instruments in the medical consultation. Twenty-seven GPs discussed in five focus groups that were digitally recorded, transcribed verbatim and analysed by systematic text condensation. Six code groups emerged from the focus group discussions: (i) a perceived pressure from authorities and psychiatry to report depression scores; (ii) the scores were considered to be of limited value for the GP but could help the patient by facilitating sick leave compensation and hospitalization; (iii) instruments hampered the dialogue with the patient and non-verbal information was lost; (iv) the reliability of questionnaires was questioned; (v) instruments were seen as not fitting into primary care and GPs were uncertain how to use them and (vi) the main advantage of instruments was to promote communication with specific categories of patients. Using instruments to obtain a quantitative score of depression was of no benefit to the GPs. Given the weak evidence for the clinical relevance of many instruments, there is little reason to introduce them into practice. However, the instruments can facilitate communication with external actors and specific groups of patients.

Chapter 6 - Lichen species to bioindicate air quality in Eastern United States from elemental composition: lessons from the Midwest

Treesearch

Susan Will-Wolf; Sarah Jovan; Michael C. Amacher

2018-01-01

Elemental concentration in lichens is a popular and cost-effective tool to bioindicate pollution load at plots (Donovan and others2016, Paoli and others 2014, Root and others 2015) and to complement costly instrumented monitoring to help assess environmental health. From recent development of lichen elemental bioindicators for air pollution in the U.S....

Stratospheric Observatory for Infrared Astronomy (SOFIA)

NASA Astrophysics Data System (ADS)

Becklin, E. E.

The joint U.S. and German SOFIA project to develop and operate a 2.5-meter infrared airborne telescope in a Boeing 747-SP is now in the final stages of development. First science flights will begin in 2008. The observatory is expected to operate for over 20 years. The sensitivity, characteristics, science instrument complement, and examples of 1-st light spectroscopic astrochemistry science are discussed.

A Qualitative Approach to the Intersection of Sexual, Ethnic, and Gender Identities.

PubMed

Narvaez, Rafael F; Meyer, Ilan H; Kertzner, Robert M; Ouellette, Suzanne C; Gordon, Allegra R

In this paper we report on a new qualitative instrument designed to study the intersection of identities related to sexuality and race/ethnicity, and how people who hold those identities interact with social contexts. Researchers often resort to using separate measures to assess race/ethnicity, sexual orientation, and other target identities. But this approach can miss elements of a self-system that stem from the intersection of identities, the interactions between identities and social contexts, related shifts in identity over time, and related changes in the prominence and valence of identities. Using a small sub-sample, we demonstrate how our instrument can help researchers overcome these limitations. Our instrument was also designed for economy in administration and analysis, so that it could be used as a qualitative complement in large survey research.

A Qualitative Approach to the Intersection of Sexual, Ethnic, and Gender Identities

PubMed Central

Narvaez, Rafael F.; Meyer, Ilan H.; Kertzner, Robert M.; Ouellette, Suzanne C.; Gordon, Allegra R.

2016-01-01

In this paper we report on a new qualitative instrument designed to study the intersection of identities related to sexuality and race/ethnicity, and how people who hold those identities interact with social contexts. Researchers often resort to using separate measures to assess race/ethnicity, sexual orientation, and other target identities. But this approach can miss elements of a self-system that stem from the intersection of identities, the interactions between identities and social contexts, related shifts in identity over time, and related changes in the prominence and valence of identities. Using a small sub-sample, we demonstrate how our instrument can help researchers overcome these limitations. Our instrument was also designed for economy in administration and analysis, so that it could be used as a qualitative complement in large survey research PMID:27683200

Ocean-atmosphere science from the NASA Plankton, Aerosol, Cloud, ocean Ecosystem (PACE) mission

NASA Astrophysics Data System (ADS)

Werdell, J.

2016-12-01

The new NASA Plankton, Aerosol, Cloud, ocean Ecosystem (PACE) mission is a strategic climate continuity activity that will not only extend key heritage ocean color, cloud, and aerosol data records, but also enable new insight into oceanographic and atmospheric responses to Earth's changing climate. The primary PACE instrument will be a spectroradiometer that spans the ultraviolet to shortwave infrared region at 5 nm resolution with a ground sample distance of 1 km at nadir. This payload will likely be complemented by a multi-angle polarimeter with a similar spectral range. Scheduled for launch in 2022, this PACE instrument pair will revolutionize studies of global biogeochemistry and carbon cycles in the ocean-atmosphere system. Here, I present a PACE mission overview, with focus on instrument characteristics, core and advanced data products, and overarching science objectives.

46 CFR 252.31 - Wages of officers and crews.

Code of Federal Regulations, 2010 CFR

2010-10-01

... Subsidy Rates § 252.31 Wages of officers and crews. (a) Definitions. When used in this part: (1) Base... the employment of the approved manning complement of the subsidized vessel, including payments... governmental assessments on crew payrolls. (4) Approved manning complement means the complement approved by the...

Potential commercial uses of EOS remote sensing products

NASA Technical Reports Server (NTRS)

Thompson, Leslie L.

1991-01-01

The instrument complement of the Earth Observing System (EOS) satellite system will generate data sets with potential interest to a variety of users who are now just beginning to develop geographic information systems tailored to their special applications and/or jurisdictions. Other users may be looking for a unique product that enhances competitive position. The generally distributed products from EOS will require additional value added processing to derive the unique products desired by specific users. Entrepreneurs have an opportunity to create these proprietary level 4 products from the EOS data sets. Specific instruments or collections of instruments could provide information for crop futures trading, mineral exploration, television and printed medium news products, regional and local government land management and planning, digital map directories, products for third world users, ocean fishing fleet probability of harvest forecasts, and other areas not even imagined at this time. The projected level 3 product are examined that will be available at launch from EOS instruments and commercial uses of the data after value added processing is estimated.

A new on-axis micro-spectrophotometer for combining Raman, fluorescence and UV/Vis absorption spectroscopy with macromolecular crystallography at the Swiss Light Source

PubMed Central

Pompidor, Guillaume; Dworkowski, Florian S. N.; Thominet, Vincent; Schulze-Briese, Clemens; Fuchs, Martin R.

2013-01-01

The combination of X-ray diffraction experiments with optical methods such as Raman, UV/Vis absorption and fluorescence spectroscopy greatly enhances and complements the specificity of the obtained information. The upgraded version of the in situ on-axis micro-spectrophotometer, MS2, at the macromolecular crystallography beamline X10SA of the Swiss Light Source is presented. The instrument newly supports Raman and resonance Raman spectroscopy, in addition to the previously available UV/Vis absorption and fluorescence modes. With the recent upgrades of the spectral bandwidth, instrument stability, detection efficiency and control software, the application range of the instrument and its ease of operation were greatly improved. Its on-axis geometry with collinear X-ray and optical axes to ensure optimal control of the overlap of sample volumes probed by each technique is still unique amongst comparable facilities worldwide and the instrument has now been in general user operation for over two years. PMID:23955041

A new on-axis micro-spectrophotometer for combining Raman, fluorescence and UV/Vis absorption spectroscopy with macromolecular crystallography at the Swiss Light Source.

PubMed

Pompidor, Guillaume; Dworkowski, Florian S N; Thominet, Vincent; Schulze-Briese, Clemens; Fuchs, Martin R

2013-09-01

The combination of X-ray diffraction experiments with optical methods such as Raman, UV/Vis absorption and fluorescence spectroscopy greatly enhances and complements the specificity of the obtained information. The upgraded version of the in situ on-axis micro-spectrophotometer, MS2, at the macromolecular crystallography beamline X10SA of the Swiss Light Source is presented. The instrument newly supports Raman and resonance Raman spectroscopy, in addition to the previously available UV/Vis absorption and fluorescence modes. With the recent upgrades of the spectral bandwidth, instrument stability, detection efficiency and control software, the application range of the instrument and its ease of operation were greatly improved. Its on-axis geometry with collinear X-ray and optical axes to ensure optimal control of the overlap of sample volumes probed by each technique is still unique amongst comparable facilities worldwide and the instrument has now been in general user operation for over two years.

Stratospheric Observatory for Infrared Astronomy (SOFIA): Infrared Sensor Development and Science Capabilities

NASA Astrophysics Data System (ADS)

Nelson, J.; Ruzek, M.

The Stratospheric Observatory for Infrared Astronomy (SOFIA) is a unique airborne observatory designed to operate in the lower stratosphere to altitudes as high as 45,000 feet and above 99.8 percent of Earths obscuring atmospheric water vapor. SOFIA's capabilities enable science and observations that will complement and extend past, present and future infrared (IR) telescopes in wavelength range, angular and spectral resolution, and observing flexibility. The joint U.S. and German SOFIA project to develop and operate a 2.5-meter infrared airborne telescope in a Boeing 747-SP is nearing readiness for for open door flights and demonstration of early science results. Flying in the stratosphere, SOFIA allows observations throughout the infrared and submillimeter region. The SOFIA instrument complement includes broadband imagers, moderate resolution spectrographs capable of resolving broad features due to dust and large molecules, and high resolution spectrometers suitable for kinematic studies of molecular and atomic gas lines at high resolution. First science flights will begin in early 2010. A great strength of SOFIA is the enormous breadth of its capabilities and the flexibility with which those capabilities can be modified and improved to take advantage of advances in infrared technology. This paper and presentation will highlight the following points: A 2.5-meter effective-diameter optical-quality telescope for diffraction-limited imaging beyond 25 micrometers, giving the sharpest view of the sky provided by any current or developmental IR telescope operating in the 30-60 micrometers region; Wavelength coverage from 0.3 micrometers to 1.6 mm and high resolution spectroscopy (R to 105) at wavelengths between 5 and 150 micrometers; An 8 arcmin FOV allowing use of very large detector arrays; Ready observer access to science instruments which can be serviced in flight and changed between flights; A low-risk ability to incorporate new science-enabling instrument technologies and to create a whole "new" observatory several times during the lifetime of the facility; Opportunity for continuous training of instrumentalists to develop and test the next generation of instrumentation for both suborbital and space applications; Mobility, which allows access to the entire sky and a vastly increased number of stellar occultation events; Unique opportunities for educators and journalists to participate first-hand in exciting astronomical observations. The mid- and far-IR wavelength regions are key to studying the dusty universe. SOFIA science emphasizes four major themes: Star and planet formation; the interstellar medium of the Milky Way; Galaxies and the galactic center; and Planetary science. These capabilities will enable a wide range of science investigations over SOFIA's 20-year operational lifetime. This paper will address SOFIA's nine first-light science instruments, capabilities, and development.

Eclipse Science Results from the Airborne Infrared Spectrometer (AIR-Spec)

NASA Astrophysics Data System (ADS)

Samra, J.; Cheimets, P.; DeLuca, E.; Golub, L.; Judge, P. G.; Lussier, L.; Madsen, C. A.; Marquez, V.; Tomczyk, S.; Vira, A.

2017-12-01

We present the first science results from the commissioning flight of the Airborne Infrared Spectrometer (AIR-Spec), an innovative solar spectrometer that will observe the 2017 solar eclipse from the NSF/NCAR High-Performance Instrumented Airborne Platform for Environmental Research (HIAPER). During the eclipse, AIR-Spec will image five magnetically sensitive coronal emission lines between 1.4 and 4 microns to determine whether they may be useful probes of coronal magnetism. The instrument will measure emission line intensity, FWHM, and Doppler shift from an altitude of over 14 km, above local weather and most of the absorbing water vapor. Instrumentation includes an image stabilization system, feed telescope, grating spectrometer, infrared camera, and visible slit-jaw imager. Results from the 2017 eclipse are presented in the context of the mission's science goals. AIR-Spec will identify line strengths as a function of position in the solar corona and search for the high frequency waves that are candidates for heating and acceleration of the solar wind. The instrument will also identify large scale flows in the corona, particularly in polar coronal holes. Three of the five lines are expected to be strong in coronal hole plasmas because they are excited in part by scattered photospheric light. Line profile analysis will probe the origins of the fast and slow solar wind. Finally, the AIR-Spec measurements will complement ground based eclipse observations to provide detailed plasma diagnostics throughout the corona. AIR-Spec will measure infrared emission of ions observed in the visible from the ground, giving insight into plasma heating and acceleration at radial distances inaccessible to existing or planned spectrometers.

MAX '91: An advanced payload for the exploration of high energy processes on the active sun

NASA Technical Reports Server (NTRS)

1986-01-01

The results of a NASA science working group established to study a follow-on to the Solar Maximum Mission are given. A complement of instruments is suggested, with the primary objective of studying the physics of energetic processes in cosmic plasmas by observing high-energy phenomena in solar flares. High-quality flare observations will be possible with these instruments during the next peak in solar activity expected to last from 1990 through at least 1995. The primary objective of MAX '91 is to study energetic processes in cosmic plasmas by observing high-energy phenomena in solar flares. These processes, which are of general astrophysical importance, include energy release, particle acceleration, and energy transport. Results from comprehensive observing programs conducted during the last solar cycle have demonstrated the great scientific potential of high-energy emissions for addressing these central physical processes. Consequently, a payload optimized for observations of high-energy solar flare phenomena is suggested for MAX '91. It consists of the following four specific instruments: (1) a Fourier-transform X-ray and gamma-ray imager covering the energy range from a few keV to 1 MeV with arcsecond spatial resolution; (2) a cooled germanium X-ray and gamma-ray spectrometer with keV spectral resolution covering the energy range from 10 keV to 50 MeV; (3) Bragg spectrometers with high spectral resolution at wavelengths between 1 and 9 angstrons; and (4) a soft X-ray, EUV, or UV imaging instrument with arcsecond spatial resolution.

46 CFR 282.21 - Wages of officers and crew.

Code of Federal Regulations, 2010 CFR

2010-10-01

...) Definitions. When used in this part. (1) Base period. The first base period under the wage index system, as... employment of the approved manning complement of the subsidized vessel, including payments required by law to... assessments on crew payrolls. (4) Approved manning complement means the complement approved by the Board for...

Global Tsunami Database: Adding Geologic Deposits, Proxies, and Tools

NASA Astrophysics Data System (ADS)

Brocko, V. R.; Varner, J.

2007-12-01

A result of collaboration between NOAA's National Geophysical Data Center (NGDC) and the Cooperative Institute for Research in the Environmental Sciences (CIRES), the Global Tsunami Database includes instrumental records, human observations, and now, information inferred from the geologic record. Deep Ocean Assessment and Reporting of Tsunamis (DART) data, historical reports, and information gleaned from published tsunami deposit research build a multi-faceted view of tsunami hazards and their history around the world. Tsunami history provides clues to what might happen in the future, including frequency of occurrence and maximum wave heights. However, instrumental and written records commonly span too little time to reveal the full range of a region's tsunami hazard. The sedimentary deposits of tsunamis, identified with the aid of modern analogs, increasingly complement instrumental and human observations. By adding the component of tsunamis inferred from the geologic record, the Global Tsunami Database extends the record of tsunamis backward in time. Deposit locations, their estimated age and descriptions of the deposits themselves fill in the tsunami record. Tsunamis inferred from proxies, such as evidence for coseismic subsidence, are included to estimate recurrence intervals, but are flagged to highlight the absence of a physical deposit. Authors may submit their own descriptions and upload digital versions of publications. Users may sort by any populated field, including event, location, region, age of deposit, author, publication type (extract information from peer reviewed publications only, if you wish), grain size, composition, presence/absence of plant material. Users may find tsunami deposit references for a given location, event or author; search for particular properties of tsunami deposits; and even identify potential collaborators. Users may also download public-domain documents. Data and information may be viewed using tools designed to extract and display data from the Oracle database (selection forms, Web Map Services, and Web Feature Services). In addition, the historic tsunami archive (along with related earthquakes and volcanic eruptions) is available in KML (Keyhole Markup Language) format for use with Google Earth and similar geo-viewers.

Complement, a target for therapy in inflammatory and degenerative diseases.

PubMed

Morgan, B Paul; Harris, Claire L

2015-12-01

The complement system is a key innate immune defence against infection and an important driver of inflammation; however, these very properties can also cause harm. Inappropriate or uncontrolled activation of complement can cause local and/or systemic inflammation, tissue damage and disease. Complement provides numerous options for drug development as it is a proteolytic cascade that involves nine specific proteases, unique multimolecular activation and lytic complexes, an arsenal of natural inhibitors, and numerous receptors that bind to activation fragments. Drug design is facilitated by the increasingly detailed structural understanding of the molecules involved in the complement system. Only two anti-complement drugs are currently on the market, but many more are being developed for diseases that include infectious, inflammatory, degenerative, traumatic and neoplastic disorders. In this Review, we describe the history, current landscape and future directions for anti-complement therapies.

Identification of C3b-binding Small Molecule Complement Inhibitors Using Cheminformatics

PubMed Central

Garcia, Brandon L.; Skaff, D. Andrew; Chatterjee, Arindam; Hanning, Anders; Walker, John K.; Wyckoff, Gerald J.; Geisbrecht, Brian V.

2017-01-01

The complement system is an elegantly regulated biochemical cascade formed by the collective molecular recognition properties and proteolytic activities of over two dozen membrane-bound or serum proteins. Complement plays diverse roles in human physiology which include acting as a sentry against invading microorganisms, priming of the adaptive immune response, and removal of immune complexes. However, dysregulation of complement can serve as a trigger for a wide range of human diseases which include autoimmune, inflammatory, and degenerative conditions. Despite several potential advantages of modulating complement with small molecule inhibitors, small molecule drugs are highly underrepresented in the current complement-directed therapeutics pipeline. In this study we have employed a cheminformatics drug discovery approach based on the extensive structural and functional knowledge available for the central proteolytic fragment of the cascade, C3b. Using parallel in silico screening methodologies we identified 45 small molecules which putatively bind C3b near ligand-guided functional hot-spots. Surface plasmon resonance experiments resulted in the validation of seven dose-dependent C3b-binding compounds. Competition-based biochemical assays demonstrated the ability of several C3b-binding compounds to interfere with binding of the original C3b ligand which guided their discovery. In vitro assays of complement function identified a single complement inhibitory compound, termed cmp-5, and mechanistic studies of the cmp-5 inhibitory mode revealed it acts at the level of C5 activation. This study has led to the identification of a promising new class of C3b-binding small molecule complement inhibitors, and to our knowledge, provides the first demonstration of cheminformatics-based complement-directed drug discovery. PMID:28298523

[Renal risks of dietary complements: a forgotten cause].

PubMed

Dori, Olympia; Humbert, Antoine; Burnier, Michel; Teta, Daniel

2014-02-26

The use of dietary complements like vitamins, minerals, trace elements, proteins, aminoacids and plant-derived agents is prevalent in the general population, in order to promote health and treat diseases. Dietary complements are considered as safe natural products and are easily available without prescription. However, these can lead to severe renal toxicity, especially in cases of unknown pre-existing chronic kidney disease (CKD). In particular, Chinese herbs including aristolochic acid, high doses of vitamine C, creatine and protein complements may lead to acute and chronic renal failure, sometimes irreversible. Dietary complement toxicity should be suspected in any case of unexplained renal impairement. In the case of pre-existing CKD, the use of potentially nephrotoxic dietary complements should be screened for.

SODART telescopes on the Spectrum X-Gamma (SRG) and their complement of instruments

NASA Astrophysics Data System (ADS)

Schnopper, Herbert W.

1994-11-01

Two SODART thin foil X-ray telescopes will fly on SRG. In the focal plane of one telescope there are: HEPC/LEPC (high- and low energy imaging proportional counters), SIXA (solid state spectrometer array), and FRD (focal plane X-ray detector). In the other, there are: another HEPC/LEPC pair, SXRP (stellar X-ray polarimeter), and another FRD. Mounted alongside and co-aligned with the SODART telescopes is TAUVEX (UV telescope). An objective Bragg spectrometer is mounted in front of one of the telescopes. These instruments and their scientific goals will be described briefly. More detailed discussions will be given by the relevant PIs in the poster session.

Technologies for Achieving Field Ubiquitous Computing

NASA Astrophysics Data System (ADS)

Nagashima, Akira

Although the term “ubiquitous” may sound like jargon used in information appliances, ubiquitous computing is an emerging concept in industrial automation. This paper presents the author's visions of field ubiquitous computing, which is based on the novel Internet Protocol IPv6. IPv6-based instrumentation will realize the next generation manufacturing excellence. This paper focuses on the following five key issues: 1. IPv6 standardization; 2. IPv6 interfaces embedded in field devices; 3. Compatibility with FOUNDATION fieldbus; 4. Network securities for field applications; and 5. Wireless technologies to complement IP instrumentation. Furthermore, the principles of digital plant operations and ubiquitous production to support the above key technologies to achieve field ubiquitous systems are discussed.

Development Tests of a Cryogenic Filter Wheel Assembly for the NIRCam Instrument

NASA Technical Reports Server (NTRS)

McCully, Sean; Clark, Charles; Schermerhorn, Michael; Trojanek, Filip; O'Hara, Mark; Williams, Jeff; Thatcher, John

2006-01-01

The James Webb Space Telescope is an infrared-optimized space telescope scheduled for launch in 201 3. Its 6.5-m diameter primary mirror will collect light from some of the first galaxies formed after the big bang. The Near Infrared camera (NIRCam) will detect the first light from these galaxies, provide the necessary tools for studying the formation of stars, aid in discovering planets around other stars, and adjust the wave front error on the primary mirror (Fig. 1). The instrument and its complement of mechanisms and optics will operate at a cryogenic temperature of 35 K. This paper describes tests and test results of the NIRCam Filter Wheel assembly prototype.

Instrumental neutron activation analysis data for cloud-water particulate samples, Mount Bamboo, Taiwan

USGS Publications Warehouse

Lin, Neng-Huei; Sheu, Guey-Rong; Wetherbee, Gregory A.; Debey, Timothy M.

2013-01-01

Cloud water was sampled on Mount Bamboo in northern Taiwan during March 22-24, 2002. Cloud-water samples were filtered using 0.45-micron filters to remove particulate material from the water samples. Filtered particulates were analyzed by instrumental neutron activation analysis (INAA) at the U.S. Geological Survey National Reactor Facility in Denver, Colorado, in February 2012. INAA elemental composition data for the particulate materials are presented. These data complement analyses of the aqueous portion of the cloud-water samples, which were performed earlier by the Department of Atmospheric Sciences, National Central University, Taiwan. The data are intended for evaluation of atmospheric transport processes and air-pollution sources in Southeast Asia.

An Advanced Commanding and Telemetry System

NASA Astrophysics Data System (ADS)

Hill, Maxwell G. G.

The Loral Instrumentation System 500 configured as an Advanced Commanding and Telemetry System (ACTS) supports the acquisition of multiple telemetry downlink streams, and simultaneously supports multiple uplink command streams for today's satellite vehicles. By using industry and federal standards, the system is able to support, without relying on a host computer, a true distributed dataflow architecture that is complemented by state-of-the-art RISC-based workstations and file servers.

Stratospheric Observatory For Infrared Astronomy (SOFIA)

NASA Astrophysics Data System (ADS)

Becklin, E. E.; Moon, L. J.

2003-06-01

The joint U.S. and German SOFIA project to develop and operate a 2.5-meter infrared airborne telescope in a Boeing 747-SP is now well into development. First science flights will begin in 2004. The observatory is expected to operate for over 20 years. The sensitivity, characteristics and science instrument complement are discussed. SOFIA will have a number of experiments related to Brown Dwarf research; some of these are discussed.

Determination of a Unique Epitope Binding Site for a Complement-Lysis- Enhancing Monoclonal Antibody, 3D12, on the Galactose Adherence Lectin of Entamoeba histolytica, Using BIAcore.

DTIC Science & Technology

1992-05-01

COMPLEMENT-LYSIS-ENHANCING MONOCLONAL ANTIBODY, 3D12, ON THE GALACTOSE ADHERENCE LECTIN OF ENTAMOEBA HISTOLYTICA, USING BIAcore Sheila J. Wood...Binding 5. FUNDING NUMBERS Site for a Complement-Lysis-Enhancing Monoclonal Antibody, 3D12, on the Galactose Adherence Lectin of Entamoeba Hiiutolitica...Mechani sms of pathogenicity used by Entamoeba histolytica to invade the bloodstream and cause liver abscess, include complement mediated lysis

Characterization of the dynamics of the atmosphere of Venus with Doppler velocimetry

NASA Astrophysics Data System (ADS)

Machado, Pedro Miguel Borges do Canto Mota

Currently the study of the Venus' atmosphere grows as a theme of major interest among the astrophysics scientific community. The most significant aspect of the general circulation of the atmosphere of Venus is its retrograde super-rotation. A complete characterization of this dynamical phenomenon is crucial for understanding its driving mechanisms. This work participates in the international effort to characterize the atmospheric dynamics of this planet in coordination with orbiter missions, in particular with Venus Express. The objectives of this study are to investigate the nature of the processes governing the super-rotation of the atmosphere of Venus using ground-based observations, thereby complementing measurements by orbiter instruments. This thesis analyzes observations of Venus made with two different instruments and Doppler velocimetry techniques. The data analysis technique allowed an unambiguous characterization of the zonal wind latitudinal profile and its temporal variability, as well as an investigation of large-scale planetary waves signature and their role in the maintenance of the zonal super-rotation, and suggest that detection and investigation of large-scale planetary waves can be carried out with this technique.These studies complement the independent observations of the european space mission Venus Express, in particular as regards the study of atmospheric super-rotation, meridional flow and its variability. (Abstract shortened by ProQuest.).

A Balloon-Borne Cloud Condensation Nuclei Counter

NASA Technical Reports Server (NTRS)

Delene, David J.; Deshler, Terry; Wechsler, Perry; Vali, Gabor A.

1997-01-01

A balloon-borne instrument was constructed for observations of vertical profiles of cloud condensation nucleus (CCN) concentrations, active at 1% supersaturation. Droplet concentration in the static thermal-gradient diffusion chamber is deduced from the amount of scattered laser light detected by a photodetector. The photodetector is calibrated using a video camera and computer system to count the number of droplets produced from NaCl aerosol. Preliminary data are available from nine early morning profiles obtained at Laramie, Wyoming, between June 1995 and January 1997. To complement the CCN measurements, instruments that measure condensation nuclei (CN) and aerosols with diameter greater than 0.30 micrometers (D(sub 0.3) were also included on the balloon package. CCN concentrations exhibited a general decrease from the surface to the top of the boundary layers, were generally uniform through well-mixed layers, and show variability above well-mixed layers. In general, the structure of the CCN profile appears to be closely related to the structure in the CN and D(sub 0.3) profiles. Summer profiles generally have CCN concentration greater than 200/cu cm up to 500 mbar, whereas winter profiles are less than 200/cu cm at all levels.

Didactical Design Material Units of Distance and Speed to Developed Mathematical Connection in Elementary School

NASA Astrophysics Data System (ADS)

Jannah, R. R.; Apriliya, S.; Karlimah

2017-03-01

This study aims to develop alternative instructional design based of barriers learning which identified by developing mathematical connection capabilities to the material unit of distance and speed. The research was conducted in the fifth grade elementary school Instructional design is complemented with a hypothetical learning trajectory in the form of a pedagogical didactic anticipation. The method used is descriptive method with qualitative approach. Techniques data collection used were observation, interviews, and documentation. The instrument used the researchers themselves are equipped with an instrument written test. The data were analyzed qualitatively to determine the student learning obstacles, then arrange hypothetical learning trajectory and pedagogical didactic anticipation. Learning obstacle are identified, it is learning obstacle related the connections between mathematical topics, learning obstacle related with other disciplines, and learning obstacle related with everyday life. The results of this research are improvement and development of didactic design in mathematics which has activities mathematical connection to the material unit of distance and speed in elementary school. The learning activities are carried out is using varied methods include method lectures, demonstrations, practice and exercise, as well as using the modified instructional media.

Dawn: An Ion-Propelled Journey to the Beginning of the Solar System

NASA Technical Reports Server (NTRS)

Brophy, John R.; Rayman, Marc D.; Pavri, Betina

2008-01-01

The Dawn mission is designed to perform a scientific investigation of the two heaviest mainbelt asteroids Vesta and Ceres. These bodies are believed to preserve records of the physical and chemical conditions present during the formation of the solar system. The mission uses an ion propulsion system to enable the single Dawn spacecraft and its complement of scientific instruments to orbit both of these asteroids. Dawn's three science instruments - the gamma ray and neutron detector, the visible and infrared mapping spectrometer, and the primary framing camera - were successfully tested after launch and are functioning normally. The ion propulsion system includes three ion thrusters of the type flown previously on NASA's Deep Space 1 mission. A minimum of two ion thrusters is necessary to accomplish the Dawn mission. Checkout of two of the ion thrusters was completed as planned within 30 days after launch. This activity confirmed that the spacecraft has two healthy ion thrusters. While further checkout activities are still in progress, the activities completed as of the end of October indicate that the spacecraft is well on its way toward being ready for the start of the thrusting-cruise phase of the mission beginning December 15th.

The Neurolab mission and biomedical engineering: a partnership for the future.

PubMed

Liskowsky, D R; Frey, M A; Sulzman, F M; White, R J; Likowsky, D R

1996-01-01

Over the last five years, with the advent of flights of U.S. Shuttle/Spacelab missions dedicated entirely to life sciences research, the opportunities for conducting serious studies that use a fully outfitted space laboratory to better understand basic biological processes have increased. The last of this series of Shuttle/Spacelab missions, currently scheduled for 1998, is dedicated entirely to neuroscience and behavioral research. The mission, named Neurolab, includes a broad range of experiments that build on previous research efforts, as well as studies related to less mature areas of space neuroscience. The Neurolab mission provides the global scientific community with the opportunity to use the space environment for investigations that exploit microgravity to increase our understanding of basic processes in neuroscience. The results from this premier mission should lead to a significant advancement in the field as a whole and to the opening of new lines of investigation for future research. Experiments under development for this mission will utilize human subjects as well as a variety of other species. The capacity to carry out detailed experiments on both human and animal subjects in space allows a diverse complement of studies that investigate functional changes and their underlying molecular, cellular, and physiological mechanisms. In order to conduct these experiments, a wide array of biomedical instrumentation will be used, including some instruments and devices being developed especially for the mission.

The Neurolab mission and biomedical engineering: a partnership for the future

NASA Technical Reports Server (NTRS)

Liskowsky, D. R.; Frey, M. A.; Sulzman, F. M.; White, R. J.; Likowsky, D. R.

1996-01-01

Over the last five years, with the advent of flights of U.S. Shuttle/Spacelab missions dedicated entirely to life sciences research, the opportunities for conducting serious studies that use a fully outfitted space laboratory to better understand basic biological processes have increased. The last of this series of Shuttle/Spacelab missions, currently scheduled for 1998, is dedicated entirely to neuroscience and behavioral research. The mission, named Neurolab, includes a broad range of experiments that build on previous research efforts, as well as studies related to less mature areas of space neuroscience. The Neurolab mission provides the global scientific community with the opportunity to use the space environment for investigations that exploit microgravity to increase our understanding of basic processes in neuroscience. The results from this premier mission should lead to a significant advancement in the field as a whole and to the opening of new lines of investigation for future research. Experiments under development for this mission will utilize human subjects as well as a variety of other species. The capacity to carry out detailed experiments on both human and animal subjects in space allows a diverse complement of studies that investigate functional changes and their underlying molecular, cellular, and physiological mechanisms. In order to conduct these experiments, a wide array of biomedical instrumentation will be used, including some instruments and devices being developed especially for the mission.

The Complement System and Adverse Pregnancy Outcomes

PubMed Central

Regal, Jean F.; Gilbert, Jeffrey S.; Burwick, Richard M.

2015-01-01

Adverse pregnancy outcomes significantly contribute to morbidity and mortality for mother and child, with lifelong health consequences for both. The innate and adaptive immune system must be regulated to insure survival of the feta allograft, and the complement system is no exception. An intact complement system optimizes placental development and function and is essential to maintain host defense and fetal survival. Complement regulation is apparent at the placental interface from early pregnancy with some degree of complement activation occurring normally throughout gestation. However, a number of pregnancy complications including early pregnancy loss, fetal growth restriction, hypertensive disorders of pregnancy and preterm birth are associated with excessive or misdirected complement activation, and are more frequent in women with inherited or acquired complement system disorders or complement gene mutations. Clinical studies employing complement biomarkers in plasma and urine implicate dysregulated complement activation in components of each of the adverse pregnancy outcomes. In addition, mechanistic studies in rat and mouse models of adverse pregnancy outcomes address the complement pathways or activation products of importance and allow critical analysis of the pathophysiology. Targeted complement therapeutics are already in use to control adverse pregnancy outcomes in select situations. A clearer understanding of the role of the complement system in both normal pregnancy and complicated or failed pregnancy will allow a rational approach to future therapeutic strategies for manipulating complement with the goal of mitigating adverse pregnancy outcomes, preserving host defense, and improving long term outcomes for both mother and child. PMID:25802092

Syntactic and Semantic Coordination in Finite Complement-Clause Constructions: A Diary-Based Case Study

ERIC Educational Resources Information Center

Köymen, Bahar; Lieven, Elena; Brandt, Silke

2016-01-01

This study investigates the coordination of matrix and subordinate clauses within finite complement-clause constructions. The data come from diary and audio recordings which include the utterances produced by an American English-speaking child, L, between the ages 1;08 and 3;05. We extracted all the finite complement-clause constructions that L…

Solar energy plant as a complement to a conventional heating system: Measurement of the storage and consumption of solar energy

NASA Astrophysics Data System (ADS)

Doering, E.; Lippe, W.

1982-08-01

The technical and economic performances of a complementary solar heating installation for a new swimming pool added to a two-floor dwelling were examined after measurements were taken over a period of 12 months and analyzed. In particular, the heat absorption and utilization were measured and modifications were carried out to improve pipe insulation and regulation of mixer valve motor running and volume flow. The collector system efficiency was evaluated at 15.4%, the proportion of solar energy of the total consumption being 6.1%. The solar plant and the measuring instruments are described and recommendations are made for improved design and performance, including enlargement of the collector surface area, further modification of the regulation system, utilization of temperature stratification in the storage tanks and avoiding mutual overshadowing of the collectors.

Elderly Care and Intrafamily Resource Allocation when Children Migrate.

PubMed

Antman, Francisca M

2012-01-01

This paper considers the intrafamily allocation of elderly care in the context of international migration where migrant children may be able to provide financial assistance to their parents, but are unable to offer physical care. To investigate the interaction between siblings, I take a non-cooperative view of family decision-making and estimate best response functions for individual physical and financial contributions as a function of siblings' contributions. I address the endogeneity of siblings' contributions and individual migration decisions by using siblings' characteristics as instrumental variables as well as models including family fixed effects. For both migrants and non-migrants, I find evidence that financial contributions function as strategic complements while siblings' time contributions operate as strategic substitutes. This suggests that children's contributions toward elderly care may be based on both strategic bequest and public good motivations.

Elderly Care and Intrafamily Resource Allocation when Children Migrate *

PubMed Central

Antman, Francisca M.

2012-01-01

This paper considers the intrafamily allocation of elderly care in the context of international migration where migrant children may be able to provide financial assistance to their parents, but are unable to offer physical care. To investigate the interaction between siblings, I take a non-cooperative view of family decision-making and estimate best response functions for individual physical and financial contributions as a function of siblings’ contributions. I address the endogeneity of siblings’ contributions and individual migration decisions by using siblings’ characteristics as instrumental variables as well as models including family fixed effects. For both migrants and non-migrants, I find evidence that financial contributions function as strategic complements while siblings’ time contributions operate as strategic substitutes. This suggests that children’s contributions toward elderly care may be based on both strategic bequest and public good motivations. PMID:22518064

The Gemini 8-Meter Telescopes Project

NASA Astrophysics Data System (ADS)

Boroson, Todd A.

1995-05-01

The Gemini 8-Meter Telescopes Project is an international partnership to build and operate two 8-meter telescopes, one on Mauna Kea, Hawaii, and one on Cerro Pachon, Chile. The telescopes will be international facilities, open to the scientific communities of the six member countries, the United States (50%), the United Kingdom (25%), Canada (15%), Chile (5%), Argentina (2.5%), and Brazil (2.5%). The telescopes are designed to exploit the best atmospheric conditions at these excellent sites. Near diffraction limited performance will be delivered at 2.2 microns and longward, with minimal degradation of the best seeing conditions at shorter wavelengths. The telescopes and facilities are designed to achieve emissivity <4% (requirement) or <2% (goal) if silver coatings are used. The instrument complement is diverse, including near- and mid-IR imagers, and near-IR and optical spectrographs. Both telescopes are equipped with f/16 articulated secondaries, and a future upgrade path to a wide-field f/6 configuration is provided. The northern telescope also includes a natural-guide-star adaptive optics system. Up to five instruments can be mounted simultaneously on the Cassegrain instrument interface. Approximately 50% of the telescope time will be flexibly scheduled, allowing most efficient utilization of the times of best conditions and facilitating programs which are difficult to schedule, such as synoptic and target-of-opportunity. First light for the Mauna Kea telescope is expected in late 1998, and for the Cerro Pachon telescope in mid-2000. This talk will report on construction progress, the instrumental capabilities, and operations strategies being considered. The Gemini 8-meter Telescopes Project is managed by the Association of Universities for Research in Astronomy (AURA), Inc. under a cooperative agreement with the National Science Foundation which serves as executive agency for the Gemini partner countries. U.S. participation in the project is through the U.S. Gemini Program, a division of the National Optical Astronomy Observatories. NOAO is operated by AURA, Inc. under cooperative agreement with the National Science Foundation.

In Situ Instrumentation for Sub-Surface Planetary Geochemistry

NASA Technical Reports Server (NTRS)

Bodnarik, J.; Evans, L.; Floyd, S.; Lim, L.; McClanahan, T.; Namkung, M.; Parsons, A.; Schweitzer, J.; Starr, R.; Trombka, J.

2010-01-01

Novel instrumentation is under development at NASA's Goddard Space Flight Center, building upon earth-based techniques for hostile environments, to infer geochemical processes important to formation and evolution of solid bodies in our Solar System. A prototype instrument, the Pulsed Neutron Generator Gamma Ray and Neutron Detectors (PNG-GRAND), has a 14 MeV pulsed neutron generator coupled with gamma ray and neutron detectors to measure quantitative elemental concentrations and bulk densities of a number of major, minor and trace elements at or below the surfaces with approximately a meter-sized spatial resolution down to depths of about 50 cm without the need to drill. PNG-GRAND's in situ a meter-scale measurements and adaptability to a variety of extreme space environments will complement orbital kilometer-scale and in-situ millimeter scale elemental and mineralogical measurements to provide a more complete picture of the geochemistry of planets, moons, asteroids and comets.

[Investigation of the allocation of equipment among the major research agencies for occupational health and medicine].

PubMed

Zhang, Lin; Wu, Zhi-Jun; Zhang, Shuang; Qin, Jian; Zhang, Xing

2011-08-01

To understand the allocation of instruments and equipment in major research institutions for occupational health and medicine in China. Questionnaire was designed for collecting the information of the equipment used in occupational health and medicine research. Questionnaires were distributed to 78 research agencies to investigate the situation of allocation of instrument and equipment. There was a great diversity of allocation in investigated agencies. The features in three kinds of agencies are different. The occupational health agencies in universities fit out the biological equipment in laboratories. The occupational health laboratories in CDCs were equipped with the chemical analytical devices. The institutes of occupational medicine were equipped with the clinical inspection instruments. The protocol of sharing resource and predominance complementation should be established among research institutions for occupational health and medicine in the same region or neighboring provinces.

Vernier caliper and micrometer computer models using Easy Java Simulation and its pedagogical design features—ideas for augmenting learning with real instruments

NASA Astrophysics Data System (ADS)

Wee, Loo Kang; Tiang Ning, Hwee

2014-09-01

This paper presents the customization of Easy Java Simulation models, used with actual laboratory instruments, to create active experiential learning for measurements. The laboratory instruments are the vernier caliper and the micrometer. Three computer model design ideas that complement real equipment are discussed. These ideas involve (1) a simple two-dimensional view for learning from pen and paper questions and the real world; (2) hints, answers, different scale options and the inclusion of zero error; (3) assessment for learning feedback. The initial positive feedback from Singaporean students and educators indicates that these tools could be successfully shared and implemented in learning communities. Educators are encouraged to change the source code for these computer models to suit their own purposes; they have creative commons attribution licenses for the benefit of all.

The ART-XC Instrument on Board the SRG Mission

NASA Technical Reports Server (NTRS)

Pavlinksy, M.; Akimov, V.; Levin, V.; Lapshov, I.; Tkachenko, A.; Semena, N.; Buntov, M.; Glushenko, A.; Arefiev, V.; Yaskovish, A.;

Precise Chemical Analyses of Planetary Surfaces

NASA Technical Reports Server (NTRS)

Kring, David; Schweitzer, Jeffrey; Meyer, Charles; Trombka, Jacob; Freund, Friedemann; Economou, Thanasis; Yen, Albert; Kim, Soon Sam; Treiman, Allan H.; Blake, David;

Research at the Institute of Astronomy and Astrophysics of the Université Libre de Bruxelles

NASA Astrophysics Data System (ADS)

Karinkuzhi, Drisya; Chamel, Nicolas; Goriely, Stéphane; Jorissen, Alain; Pourbaix, Dimitri; Siess, Lionel; Van Eck, Sophie

2018-04-01

Over the years, a coherent research strategy has developed in the field of stellar physics at the Institute of Astronomy and Astrophysics (IAA). It involves observational studies (chemical composition of giant stars, binary properties, tomography of stellar atmospheres) that make use of the large ESO telescopes as well as of other major instruments. The presence of a high-resolution spectrograph on the 3.6-m Devasthal Optical Telescope (DOT) would therefore be highly beneficial to IAA research. These observations are complemented and supported by theoretical studies of mass transfer in binary systems, of standard and non-standard stellar evolution (including the modelling of stellar hydrodynamical nuclear burning for application to certain thermonuclear supernovae) and of nuclear astrophysics (a field in which IAA has been recognized for a long time as an international centre of excellence), including the theory of nucleosynthesis. IAA also addresses the end-points of stellar evolution as it is carrying out research on the compact remnants of stellar evolution of massive stars: neutron stars.

Non-specific adsorption of complement proteins affects complement activation pathways of gold nanomaterials.

PubMed

Quach, Quang Huy; Kah, James Chen Yong

2017-04-01

The complement system is a key humoral component of innate immunity, serving as the first line of defense against intruders, including foreign synthetic nanomaterials. Although gold nanomaterials (AuNMs) are widely used in nanomedicine, their immunological response is not well understood. Using AuNMs of three shapes commonly used in biomedical applications: spherical gold nanoparticles, gold nanostars and gold nanorods, we demonstrated that AuNMs activated whole complement system, leading to the formation of SC5b-9 complex. All three complement pathways were simultaneously activated by all the AuNMs. Recognition molecules of the complement system interacted with all AuNMs in vitro, except for l-ficolin, but the correlation between these interactions and corresponding complement pathway activation was only observed in the classical and alternative pathways. We also observed the mediating role of complement activation in cellular uptake of all AuNMs by human U937 promonocytic cells, which expresses complement receptors. Taken together, our results highlighted the potential immunological challenges for clinical applications of AuNMs that were often overlooked.

Complement-Mediated Regulation of Metabolism and Basic Cellular Processes.

PubMed

Hess, Christoph; Kemper, Claudia

2016-08-16

Complement is well appreciated as a critical arm of innate immunity. It is required for the removal of invading pathogens and works by directly destroying them through the activation of innate and adaptive immune cells. However, complement activation and function is not confined to the extracellular space but also occurs within cells. Recent work indicates that complement activation regulates key metabolic pathways and thus can impact fundamental cellular processes, such as survival, proliferation, and autophagy. Newly identified functions of complement include a key role in shaping metabolic reprogramming, which underlies T cell effector differentiation, and a role as a nexus for interactions with other effector systems, in particular the inflammasome and Notch transcription-factor networks. This review focuses on the contributions of complement to basic processes of the cell, in particular the integration of complement with cellular metabolism and the potential implications in infection and other disease settings. Copyright © 2016 Elsevier Inc. All rights reserved.

Using animal models to determine the significance of complement activation in Alzheimer's disease

PubMed Central

Loeffler, David A

2004-01-01

Complement inflammation is a major inflammatory mechanism whose function is to promote the removal of microorganisms and the processing of immune complexes. Numerous studies have provided evidence for an increase in this process in areas of pathology in the Alzheimer's disease (AD) brain. Because complement activation proteins have been demonstrated in vitro to exert both neuroprotective and neurotoxic effects, the significance of this process in the development and progression of AD is unclear. Studies in animal models of AD, in which brain complement activation can be experimentally altered, should be of value for clarifying this issue. However, surprisingly little is known about complement activation in the transgenic animal models that are popular for studying this disorder. An optimal animal model for studying the significance of complement activation on Alzheimer's – related neuropathology should have complete complement activation associated with senile plaques, neurofibrillary tangles (if present), and dystrophic neurites. Other desirable features include both classical and alternative pathway activation, increased neuronal synthesis of native complement proteins, and evidence for an increase in complement activation prior to the development of extensive pathology. In order to determine the suitability of different animal models for studying the role of complement activation in AD, the extent of complement activation and its association with neuropathology in these models must be understood. PMID:15479474

Determination of Root Canal Cleanliness by Different Irrigation Methods and Morphometric Analysis of Apical Third.

PubMed

Seixas, Fábio Heredia; Estrela, Carlos; Bueno, Mike Reis; Sousa-Neto, Manoel Damião; Pécora, Jesus Djalma

2015-06-01

The aim of this study was to determine the root canal area before and after the instrumentation 1 mm short of the apical foramen by clinical and cone beam computed tomography (CBCT) methods, and to evaluate the cleanliness of the apical region in mesiodistal flattened teeth by using optical microscopy. Forty-two human single-canal mandibular incisors were instrumented using the Free Tip Preparation technique up to three, four or five instruments from the initial. Cone beam computed tomography scans were acquired of the samples before and after root canal preparation (RCP). Irrigation was performed by conventional or hydrodynamic means, using 2.5% sodium hypochlorite. The samples were prepared for observation under an optical microscope. Images were digitally obtained, analyzed and the results were submitted to statistical analysis (two-way ANOVA complemented by Bonferroni's post-test). There was no significant difference between the studied anatomical areas with both CBCT and clinical methods. There were no differences between irrigation methods. It was verified differences between instrumentation techniques. Instrumentation with four instruments from the initial instrument determined a significant increase in the contact area when compared to preparation with three instruments, but RCP with 5 instruments did not result in a better cleanliness. The analysis with CBCT was not capable to determine the precise shape of surgical apical area comparing to the clinical method. Both the conventional and hydrodynamic irrigation techniques were not able to promote root canals debris-free. The instruments action in root canal walls was proportional to the number of instruments used from the initial apical instrument.

Evaluating Secondary Students' Scientific Reasoning in Genetics Using a Two-Tier Diagnostic Instrument

NASA Astrophysics Data System (ADS)

Tsui, Chi-Yan; Treagust, David

2010-05-01

While genetics has remained as one key topic in school science, it continues to be conceptually and linguistically difficult for students with the concomitant debates as to what should be taught in the age of biotechnology. This article documents the development and implementation of a two-tier multiple-choice instrument for diagnosing grades 10 and 12 students' understanding of genetics in terms of reasoning. The pretest and posttest forms of the diagnostic instrument were used alongside other methods in evaluating students' understanding of genetics in a case-based qualitative study on teaching and learning with multiple representations in three Western Australian secondary schools. Previous studies have shown that a two-tier diagnostic instrument is useful in probing students' understanding or misunderstanding of scientific concepts and ideas. The diagnostic instrument in this study was designed and then progressively refined, improved, and implemented to evaluate student understanding of genetics in three case schools. The final version of the instrument had Cronbach's alpha reliability of 0.75 and 0.64, respectively, for its pretest and the posttest forms when it was administered to a group of grade 12 students (n = 17). This two-tier diagnostic instrument complemented other qualitative data collection methods in this research in generating a more holistic picture of student conceptual learning of genetics in terms of scientific reasoning. Implications of the findings of this study using the diagnostic instrument are discussed.

The Role of Magnetic Fields in Star Formation

NASA Astrophysics Data System (ADS)

Pipher, Judith

2018-06-01

The SOFIA instrument complement makes available the capability to characterize the physical properties (turbulence, dynamics, magnetic field structure and strength, gas density) of the molecular cloud filaments in which stars form.HAWC+, the newest SOFIA instrument, provides a unique opportunity to probe the complex roles that magnetic fields play in the star formation process on spatial scales intermediate to those explored by Planck (5’ scale), to those of ALMA at the smallest spatial scales (<0.6”scale and 2” fields of view). HAWC+ measures the thermal emission from dust grains in molecular cloud star forming regions at wavelengths 53 to 216 mm, as well as the far infrared polarization on diffraction-limited spatial scales of 5” – 20” over fields of view ~2’ – 8’. Complementary near- to mid-IR polarimetry on 8-10m telescopes is available, as is submm polarimetry from both ground-based and balloon-borne telescopes. Currently there is no other far-IR polarimetry facility, and the HAWC+ wavelength ranges allow discrimination among different polarization mechanisms. HAWC+’ angular resolution is particularly well suited to study the magnetic field of entire cloud cores, thus connecting the Planck large scale molecular cloud structure with ALMA’s detailed small-scale structure of the core.SOFIA also offers the advantages of molecular line emission follow-up on regions for which HAWC+ determines magnetic field strength and direction. GREAT and/or FIFI-LS molecular line observations of the region of interest will complement the magnetic field observations: cloud and filament dynamics, the magnitude of the turbulence, and of course the core gas density can be determined through observations of appropriate molecular lines.These observations, as well as synergistic observations with other telescopes, will provide powerful tools to further our understanding of the fundamental physics of both low mass and high mass star formation, including the role that magnetic fields play in each.

Hijacking Complement Regulatory Proteins for Bacterial Immune Evasion.

PubMed

Hovingh, Elise S; van den Broek, Bryan; Jongerius, Ilse

2016-01-01

The human complement system plays an important role in the defense against invading pathogens, inflammation and homeostasis. Invading microbes, such as bacteria, directly activate the complement system resulting in the formation of chemoattractants and in effective labeling of the bacteria for phagocytosis. In addition, formation of the membrane attack complex is responsible for direct killing of Gram-negative bacteria. In turn, bacteria have evolved several ways to evade complement activation on their surface in order to be able to colonize and invade the human host. One important mechanism of bacterial escape is attraction of complement regulatory proteins to the microbial surface. These molecules are present in the human body for tight regulation of the complement system to prevent damage to host self-surfaces. Therefore, recruitment of complement regulatory proteins to the bacterial surface results in decreased complement activation on the microbial surface which favors bacterial survival. This review will discuss recent advances in understanding the binding of complement regulatory proteins to the bacterial surface at the molecular level. This includes, new insights that have become available concerning specific conserved motives on complement regulatory proteins that are favorable for microbial binding. Finally, complement evasion molecules are of high importance for vaccine development due to their dominant role in bacterial survival, high immunogenicity and homology as well as their presence on the bacterial surface. Here, the use of complement evasion molecules for vaccine development will be discussed.

Hijacking Complement Regulatory Proteins for Bacterial Immune Evasion

PubMed Central

Hovingh, Elise S.; van den Broek, Bryan; Jongerius, Ilse

2016-01-01

The human complement system plays an important role in the defense against invading pathogens, inflammation and homeostasis. Invading microbes, such as bacteria, directly activate the complement system resulting in the formation of chemoattractants and in effective labeling of the bacteria for phagocytosis. In addition, formation of the membrane attack complex is responsible for direct killing of Gram-negative bacteria. In turn, bacteria have evolved several ways to evade complement activation on their surface in order to be able to colonize and invade the human host. One important mechanism of bacterial escape is attraction of complement regulatory proteins to the microbial surface. These molecules are present in the human body for tight regulation of the complement system to prevent damage to host self-surfaces. Therefore, recruitment of complement regulatory proteins to the bacterial surface results in decreased complement activation on the microbial surface which favors bacterial survival. This review will discuss recent advances in understanding the binding of complement regulatory proteins to the bacterial surface at the molecular level. This includes, new insights that have become available concerning specific conserved motives on complement regulatory proteins that are favorable for microbial binding. Finally, complement evasion molecules are of high importance for vaccine development due to their dominant role in bacterial survival, high immunogenicity and homology as well as their presence on the bacterial surface. Here, the use of complement evasion molecules for vaccine development will be discussed. PMID:28066340

Radiometric and spectral validation of Atmospheric Infrared Sounder observations with the aircraft-based Scanning High-Resolution Interferometer Sounder

NASA Astrophysics Data System (ADS)

Tobin, David C.; Revercomb, Henry E.; Knuteson, Robert O.; Best, Fred A.; Smith, William L.; Ciganovich, Nick N.; Dedecker, Ralph G.; Dutcher, Steven; Ellington, Scott D.; Garcia, Raymond K.; Howell, H. Benjamin; Laporte, Daniel D.; Mango, Stephen A.; Pagano, Thomas S.; Taylor, Joe K.; van Delst, Paul; Vinson, Kenneth H.; Werner, Mark W.

2006-05-01

The ability to accurately validate high-spectral resolution infrared radiance measurements from space using comparisons with a high-altitude aircraft spectrometer has been successfully demonstrated. The demonstration is based on a 21 November 2002 underflight of the AIRS on the NASA Aqua spacecraft by the Scanning-HIS on the NASA ER-2 high-altitude aircraft. A comparison technique which accounts for the different viewing geometries and spectral characteristics of the two sensors is introduced, and accurate comparisons are made for AIRS channels throughout the infrared spectrum. Resulting brightness temperature differences are found to be 0.2 K or less for most channels. Both the AIRS and the Scanning-HIS calibrations are expected to be very accurate (formal 3-sigma estimates are better than 1 K absolute brightness temperature for a wide range of scene temperatures), because high spectral resolution offers inherent advantages for absolute calibration and because they make use of high-emissivity cavity blackbodies as onboard radiometric references. AIRS also has the added advantage of a cold space view, and the Scanning-HIS calibration has recently benefited from the availability of a zenith view from high-altitude flights. Aircraft comparisons of this type provide a mechanism for periodically testing the absolute calibration of spacecraft instruments with instrumentation for which the calibration can be carefully maintained on the ground. This capability is especially valuable for assuring the long-term consistency and accuracy of climate observations, including those from the NASA EOS spacecraft (Terra, Aqua and Aura) and the new complement of NPOESS operational instruments. The validation role for accurately calibrated aircraft spectrometers also includes application to broadband instruments and linking the calibrations of similar instruments on different spacecraft. It is expected that aircraft flights of the Scanning-HIS and its close cousin the NPOESS Airborne Sounder Test Bed (NAST) will be used to check the long-term stability of AIRS and the NPOESS operational follow-on sounder, the Cross-track Infrared Sounder (CrIS), over the life of the missions.

Complement in the Initiation and Evolution of Rheumatoid Arthritis

PubMed Central

Holers, V. Michael; Banda, Nirmal K.

2018-01-01

The complement system is a major component of the immune system and plays a central role in many protective immune processes, including circulating immune complex processing and clearance, recognition of foreign antigens, modulation of humoral and cellular immunity, removal of apoptotic and dead cells, and engagement of injury resolving and tissue regeneration processes. In stark contrast to these beneficial roles, however, inadequately controlled complement activation underlies the pathogenesis of human inflammatory and autoimmune diseases, including rheumatoid arthritis (RA) where the cartilage, bone, and synovium are targeted. Recent studies of this disease have demonstrated that the autoimmune response evolves over time in an asymptomatic preclinical phase that is associated with mucosal inflammation. Notably, experimental models of this disease have demonstrated that each of the three major complement activation pathways plays an important role in recognition of injured joint tissue, although the lectin and amplification pathways exhibit particularly impactful roles in the initiation and amplification of damage. Herein, we review the complement system and focus on its multi-factorial role in human patients with RA and experimental murine models. This understanding will be important to the successful integration of the emerging complement therapeutics pipeline into clinical care for patients with RA. PMID:29892280

Genetic control of the alternative pathway of complement in humans and age-related macular degeneration

PubMed Central

Hecker, Laura A.; Edwards, Albert O.; Ryu, Euijung; Tosakulwong, Nirubol; Baratz, Keith H.; Brown, William L.; Issa, Peter Charbel; Scholl, Hendrik P.; Pollok-Kopp, Beatrix; Schmid-Kubista, Katharina E.; Bailey, Kent R.; Oppermann, Martin

2010-01-01

Activation of the alternative pathway of complement is implicated in common neurodegenerative diseases including age-related macular degeneration (AMD). We explored the impact of common variation in genes encoding proteins of the alternative pathway on complement activation in human blood and in AMD. Genetic variation across the genes encoding complement factor H (CFH), factor B (CFB) and component 3 (C3) was determined. The influence of common haplotypes defining transcriptional and translational units on complement activation in blood was determined in a quantitative genomic association study. Individual haplotypes in CFH and CFB were associated with distinct and novel effects on plasma levels of precursors, regulators and activation products of the alternative pathway of complement in human blood. Further, genetic variation in CFH thought to influence cell surface regulation of complement did not alter plasma complement levels in human blood. Plasma markers of chronic activation (split-products Ba and C3d) and an activating enzyme (factor D) were elevated in AMD subjects. Most of the elevation in AMD was accounted for by the genetic variation controlling complement activation in human blood. Activation of the alternative pathway of complement in blood is under genetic control and increases with age. The genetic variation associated with increased activation of complement in human blood also increased the risk of AMD. Our data are consistent with a disease model in which genetic variation in the complement system increases the risk of AMD by a combination of systemic complement activation and abnormal regulation of complement activation in local tissues. PMID:19825847

The Role of Cloud and Precipitation Radars in Convoys and Constellations

NASA Technical Reports Server (NTRS)

Tanelli, Simone; Durden, Stephen L.; Im, Eastwood; Sadowy, Gregory A.

2013-01-01

We provide an overview of which benefits a radar, and only a radar, can provide to any constellation of satellites monitoring Earth's atmosphere; which aspects instead are most useful to complement a radar instrument to provide accurate and complete description of the state of the troposphere; and finally which goals can be given a lower priority assuming that other types of sensors will be flying in formation with a radar.

Synergistic observations of convective cloud life-cycle during the Mid-latitude Continental Convective Clouds Experiment (MC3E)

NASA Astrophysics Data System (ADS)

Jensen, M. P.; Petersen, W. A.; Giangrande, S.; Heymsfield, G. M.; Kollias, P.; Rutledge, S. A.; Schwaller, M.; Zipser, E. J.

2011-12-01

The Midlatitude Continental Convective Clouds Experiment (MC3E) took place from 22 April through 6 June 2011 centered at the U.S. Department of Energy's Atmospheric Radiation Measurement (ARM) Southern Great Plains Central Facility in north-central Oklahoma. This campaign was a joint effort between the ARM and the National Aeronautics and Space Administration's (NASA) Global Precipitation Measurement mission Ground Validation program. It was the first major field campaign to take advantage of numerous new radars and other remote sensing instrumentation purchased through the American Recovery and Reinvestment Act of 2009. The measurement strategy for this field campaign was to provide a well-defined forcing dataset for modeling efforts coupled with detailed observations of cloud/precipitation dynamics and microphysics within the domain highlighted by advanced multi-scale, multi-frequency radar remote sensing. These observations are aimed at providing important insights into eight different components of convective simulation and microphysical parameterization: (1) pre-convective environment, (2) convective initiation, (3) updraft/downdraft dynamics, (4) condensate transport/detrainment/entrainment, (5) precipitation and cloud microphysics, (6) influence on the environment, (7) influence on radiation, and (8) large-scale forcing. In order to obtain the necessary dataset, the MC3E surface-based observational network included six radiosonde launch sites each launching 4-8 sondes per day, three X-band scanning ARM precipitation radars, a C-band scanning ARM precipitation radar, the NASA N-Pol (S-band) scanning radar, the NASA D3R Ka/Ku-band radar, the Ka/W-band scanning ARM cloud radar, vertically pointing radar systems at Ka-, S- and UHF band, a network of over 20 disdrometers and rain gauges and the full complement of radiation, cloud and atmospheric state observations available at the ARM facility. This surface-based network was complemented by aircraft measurements by the NASA ER-2 high altitude aircraft which included a radar system (Ka/Ku band) and multiple passive microwave radiometers (10-183 GHz) and the University of North Dakota Citation which included a full suite of in situ microphysics instruments. The campaign was successful in providing observations over a wide variety of convective cloud types, from shallow non-precipitating cloud fields to shallow-to-deep transitions to mature deep convective systems some of which included severe weather. We will present an overview of the convective cloud conditions that were observed, the status MC3E datastreams and a summary of some of the preliminary results.

Assessing hospitals' clinical risk management: Development of a monitoring instrument

PubMed Central

2010-01-01

Background Clinical risk management (CRM) plays a crucial role in enabling hospitals to identify, contain, and manage risks related to patient safety. So far, no instruments are available to measure and monitor the level of implementation of CRM. Therefore, our objective was to develop an instrument for assessing CRM in hospitals. Methods The instrument was developed based on a literature review, which identified key elements of CRM. These elements were then discussed with a panel of patient safety experts. A theoretical model was used to describe the level to which CRM elements have been implemented within the organization. Interviews with CRM practitioners and a pilot evaluation were conducted to revise the instrument. The first nationwide application of the instrument (138 participating Swiss hospitals) was complemented by in-depth interviews with 25 CRM practitioners in selected hospitals, for validation purposes. Results The monitoring instrument consists of 28 main questions organized in three sections: 1) Implementation and organizational integration of CRM, 2) Strategic objectives and operational implementation of CRM at hospital level, and 3) Overview of CRM in different services. The instrument is available in four languages (English, German, French, and Italian). It allows hospitals to gather comprehensive and systematic data on their CRM practice and to identify areas for further improvement. Conclusions We have developed an instrument for assessing development stages of CRM in hospitals that should be feasible for a continuous monitoring of developments in this important area of patient safety. PMID:21144039

Detection of pigments of halophilic endoliths from gypsum: Raman portable instrument and European Space Agency's prototype analysis

PubMed Central

Culka, Adam; Osterrothová, Kateřina; Hutchinson, Ian; Ingley, Richard; McHugh, Melissa; Oren, Aharon; Edwards, Howell G. M.; Jehlička, Jan

2014-01-01

A prototype instrument, under development at the University of Leicester, for the future European Space Agency (ESA) ExoMars mission, was used for the analysis of microbial pigments within a stratified gypsum crust from a hypersaline saltern evaporation pond at Eilat (Israel). Additionally, the same samples were analysed using a miniaturized Raman spectrometer, featuring the same 532 nm excitation. The differences in the position of the specific bands, attributed to carotenoid pigments from different coloured layers, were minor when analysed by the ESA prototype instrument; therefore, making it difficult to distinguish among the different pigments. The portable Delta Nu Advantage instrument allowed for the discrimination of microbial carotenoids from the orange/green and purple layers. The purpose of this study was to complement previous laboratory results with new data and experience with portable or handheld Raman systems, even with a dedicated prototype Raman system for the exploration of Mars. The latter is equipped with an excitation wavelength falling within the carotenoid polyene resonance region. The ESA prototype Raman instrument detected the carotenoid pigments (biomarkers) with ease, although further detailed distinctions among them were not achieved. PMID:25368354

The renaissance of complement therapeutics

PubMed Central

Ricklin, Daniel; Mastellos, Dimitrios C.; Reis, Edimara S.; Lambris, John D.

2018-01-01

The increasing number of clinical conditions that involve a pathological contribution from the complement system — many of which affect the kidneys — has spurred a regained interest in therapeutic options to modulate this host defence pathway. Molecular insight, technological advances, and the first decade of clinical experience with the complement-specific drug eculizumab, have contributed to a growing confidence in therapeutic complement inhibition. More than 20 candidate drugs that target various stages of the complement cascade are currently being evaluated in clinical trials, and additional agents are in preclinical development. Such diversity is clearly needed in view of the complex and distinct involvement of complement in a wide range of clinical conditions, including rare kidney disorders, transplant rejection and haemodialysis-induced inflammation. The existing drugs cannot be applied to all complement-driven diseases, and each indication has to be assessed individually. Alongside considerations concerning optimal points of intervention and economic factors, patient stratification will become essential to identify the best complement-specific therapy for each individual patient. This Review provides an overview of the therapeutic concepts, targets and candidate drugs, summarizes insights from clinical trials, and reflects on existing challenges for the development of complement therapeutics for kidney diseases and beyond. PMID:29199277

Complement in Non-Antibody-Mediated Kidney Diseases

PubMed Central

Angeletti, Andrea; Reyes-Bahamonde, Joselyn; Cravedi, Paolo; Campbell, Kirk N.

2017-01-01

The complement system is part of the innate immune response that plays important roles in protecting the host from foreign pathogens. The complement components and relative fragment deposition have long been recognized to be strongly involved also in the pathogenesis of autoantibody-related kidney glomerulopathies, leading to direct glomerular injury and recruitment of infiltrating inflammation pathways. More recently, unregulated complement activation has been shown to be associated with progression of non-antibody-mediated kidney diseases, including focal segmental glomerulosclerosis, C3 glomerular disease, thrombotic microangiopathies, or general fibrosis generation in progressive chronic kidney diseases. Some of the specific mechanisms associated with complement activation in these diseases were recently clarified, showing a dominant role of alternative activation pathway. Over the last decade, a growing number of anticomplement agents have been developed, and some of them are being approved for clinical use or already in use. Therefore, anticomplement therapies represent a realistic choice of therapeutic approaches for complement-related diseases. Herein, we review the complement system activation, regulatory mechanisms, their involvement in non-antibody-mediated glomerular diseases, and the recent advances in complement-targeting agents as potential therapeutic strategies. PMID:28748184

Accurate, Sensitive, and Precise Multiplexed Proteomics Using the Complement Reporter Ion Cluster

DOE PAGES

Sonnett, Matthew; Yeung, Eyan; Wuhr, Martin

2018-03-09

We present that quantitative analysis of proteomes across multiple time points, organelles, and perturbations is essential for understanding both fundamental biology and disease states. The development of isobaric tags (e.g. TMT) have enabled the simultaneous measurement of peptide abundances across several different conditions. These multiplexed approaches are promising in principle because of advantages in throughput and measurement quality. However, in practice existing multiplexing approaches suffer from key limitations. In its simple implementation (TMT-MS2), measurements are distorted by chemical noise leading to poor measurement accuracy. The current state-of-the-art (TMT-MS3) addresses this, but requires specialized quadrupole-iontrap-Orbitrap instrumentation. The complement reporter ion approachmore » (TMTc) produces high accuracy measurements and is compatible with many more instruments, like quadrupole-Orbitraps. However, the required deconvolution of the TMTc cluster leads to poor measurement precision. Here, we introduce TMTc+, which adds the modeling of the MS2-isolation step into the deconvolution algorithm. The resulting measurements are comparable in precision to TMT-MS3/MS2. The improved duty cycle, and lower filtering requirements make TMTc+ more sensitive than TMT-MS3 and comparable with TMT-MS2. At the same time, unlike TMT-MS2, TMTc+ is exquisitely able to distinguish signal from chemical noise even outperforming TMT-MS3. Lastly, we compare TMTc+ to quantitative label-free proteomics of total HeLa lysate and find that TMTc+ quantifies 7.8k versus 3.9k proteins in a 5-plex sample. At the same time the median coefficient of variation improves from 13% to 4%. Furthermore, TMTc+ advances quantitative proteomics by enabling accurate, sensitive, and precise multiplexed experiments on more commonly used instruments.« less

Accurate, Sensitive, and Precise Multiplexed Proteomics Using the Complement Reporter Ion Cluster

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sonnett, Matthew; Yeung, Eyan; Wuhr, Martin

We present that quantitative analysis of proteomes across multiple time points, organelles, and perturbations is essential for understanding both fundamental biology and disease states. The development of isobaric tags (e.g. TMT) have enabled the simultaneous measurement of peptide abundances across several different conditions. These multiplexed approaches are promising in principle because of advantages in throughput and measurement quality. However, in practice existing multiplexing approaches suffer from key limitations. In its simple implementation (TMT-MS2), measurements are distorted by chemical noise leading to poor measurement accuracy. The current state-of-the-art (TMT-MS3) addresses this, but requires specialized quadrupole-iontrap-Orbitrap instrumentation. The complement reporter ion approachmore » (TMTc) produces high accuracy measurements and is compatible with many more instruments, like quadrupole-Orbitraps. However, the required deconvolution of the TMTc cluster leads to poor measurement precision. Here, we introduce TMTc+, which adds the modeling of the MS2-isolation step into the deconvolution algorithm. The resulting measurements are comparable in precision to TMT-MS3/MS2. The improved duty cycle, and lower filtering requirements make TMTc+ more sensitive than TMT-MS3 and comparable with TMT-MS2. At the same time, unlike TMT-MS2, TMTc+ is exquisitely able to distinguish signal from chemical noise even outperforming TMT-MS3. Lastly, we compare TMTc+ to quantitative label-free proteomics of total HeLa lysate and find that TMTc+ quantifies 7.8k versus 3.9k proteins in a 5-plex sample. At the same time the median coefficient of variation improves from 13% to 4%. Furthermore, TMTc+ advances quantitative proteomics by enabling accurate, sensitive, and precise multiplexed experiments on more commonly used instruments.« less

Measuring Gases Using Drones at Turrialba Volcano, Costa Rica

NASA Astrophysics Data System (ADS)

Stix, J.; Alan, A., Jr.; Corrales, E.; D'Arcy, F.; de Moor, M. J.; Diaz, J. A.

2016-12-01

We are currently developing a series of drones and associated instrumentation to study Turrialba volcano in Costa Rica. This volcano has shown increasing activity during the last 20 years, and the volcano is currently in a state of heightened unrest as exemplified by recent explosive activity in May-August 2016. The eruptive activity has made the summit area inaccessible to normal gas monitoring activities, prompting development of new techniques to measure gas compositions. We have been using two drones, a DJI Spreading Wings S1000 octocopter and a Turbo Ace Matrix-i quadcopter, to airlift a series of instruments to measure volcanic gases in the plume of the volcano. These instruments comprise optical and electrochemical sensors to measure CO2, SO2, and H2S concentrations which are considered the most significant species to help forecast explosive eruptions and determine the relative proportions of magmatic and hydrothermal components in the volcanic gas. Additionally, cameras and sensors to measure air temperature, relative humidity, atmospheric pressure, and GPS location are included in the package to provide meteorological and geo-referenced information to complement the concentration data and provide a better picture of the volcano from a remote location. The integrated payloads weigh 1-2 kg, which can typically be flown by the drones in 10-20 minutes at altitudes of 2000-4000 meters. Preliminary tests at Turrialba in May 2016 have been very encouraging, and we are in the process of refining both the drones and the instrumentation packages for future flights. Our broader goals are to map gases in detail with the drones in order to make flux measurements of each species, and to apply this approach at other volcanoes.

HMMR (High-Resolution Multifrequency Microwave Radiometer) Earth observing system, volume 2e. Instrument panel report

NASA Technical Reports Server (NTRS)

1987-01-01

Recommendations and background are provided for a passive microwave remote sensing system of the future designed to meet the observational needs of Earth scientist in the next decade. This system, called the High Resolution Multifrequency Microwave Radiometer (HMMR), is to be part of a complement of instruments in polar orbit. Working together, these instruments will form an Earth Observing System (EOS) to provide the information needed to better understand the fundamental, global scale processes which govern the Earth's environment. Measurements are identified in detail which passive observations in the microwave portion of the spectrum could contribute to an Earth Observing System in polar orbit. Requirements are established, e.g., spatial and temporal resolution, for these measurements so that, when combined with the other instruments in the Earth Observing System, they would yield a data set suitable for understanding the fundamental processes governing the Earth's environment. Existing and/or planned sensor systems are assessed in the light of these requirements, and additional sensor hardware needed to meet these observational requirements are defined.

Low-cost thermal-IR imager for an Earth observation microsatellite

NASA Astrophysics Data System (ADS)

Oelrich, Brian D.; Underwood, Craig I.

2017-11-01

A new class of thermal infrared (TIR) Earth Observation (EO) data will become available with the flight of miniature TIR EO instruments in a multiple micro-satellite constellation. This data set will provide a unique service for those wishing to analyse trends or rapidly detect anomalous changes in the TIR characteristics of the Earth's surface or atmosphere (e.g. fire detection). Following a preliminary study of potential mission applications, uncooled commercial-off-the-shelf (COTS) technology was selected to form the basis of a low-cost, compact instrument capable of complementing existing visible and near IR EO capabilities on a sub-100kg Surrey micro-satellite. The preliminary 2-3 kg instrument concept has been designed to yield a 325 m ground sample distance over a 200 km swath width from a constellation altitude of 700 km. The radiometric performance, enhanced with time-delayed integration (TDI), is expected to yield a NETD less than 0.5 K for a 300 K ground scene. Fabrication and characterization of a space-ready instrument is planned for late 2004.

Spallation Neutron Source Second Target Station Integrated Systems Update

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ankner, John Francis; An, Ke; Blokland, Willem

The Spallation Neutron Source (SNS) was designed from the beginning to accommodate both an accelerator upgrade to increase the proton power and a second target station (STS). Four workshops were organized in 2013 and 2014 to identify key science areas and challenges where neutrons will play a vital role [1-4]. Participants concluded that the addition of STS to the existing ORNL neutron sources was needed to complement the strengths of High Flux Isotope Reactor (HFIR) and the SNS first target station (FTS). To address the capability gaps identified in the workshops, a study was undertaken to identify instrument concepts thatmore » could provide the required new science capabilities. The study outlined 22 instrument concepts and presented an initial science case for STS [5]. These instrument concepts formed the basis of a planning suite of instruments whose requirements determined an initial site layout and moderator selection. An STS Technical Design Report (TDR) documented the STS concept based on those choices [6]. Since issue of the TDR, the STS concept has significantly matured as described in this document.« less

Evasion of Complement-Mediated Lysis and Complement C3 Deposition Are Regulated by Francisella tularensis Lipopolysaccharide O Antigen1

PubMed Central

Clay, Corey D.; Soni, Shilpa; Gunn, John S.; Schlesinger, Larry S.

2009-01-01

The bacterium Francisella tularensis (Ft) is a potential weapon of bioterrorism when aerosolized. Macrophage infection is necessary for disease progression and efficient phagocytosis by human macrophages requires serum opsonization by complement. Microbial complement activation leads to surface deposition of a highly regulated protein complex resulting in opsonization or membrane lysis. The nature of complement component C3 deposition, i.e., C3b (opsonization and lysis) or C3bi (opsonization only) fragment deposition, is central to the outcome of activation. In this study, we examine the mechanisms of Ft resistance to complement-mediated lysis, C3 component deposition on the Ft surface, and complement activation. Upon incubation in fresh nonimmune human serum, Schu S4 (Ft subsp. tularensis), Fn (Ft subsp. novicida), and LVS (Ft subsp. holarctica live vaccine strain) were resistant to complement-mediated lysis, but LVSG and LVSR (LVS strains altered in surface carbohydrate structures) were susceptible. C3 deposition, however, occurred on all strains. Complement-susceptible strains had markedly increased C3 fragment deposition, including the persistent presence of C3b compared with C3bi, which indicates that C3b inactivation results in survival of complement-resistant strains. C1q, an essential component of the classical activation pathway, was necessary for lysis of complement-susceptible strains and optimal C3 deposition on all strains. Finally, use of Francisella LPS mutants confirmed O Ag as a major regulator of complement resistance. These data provide evidence that pathogenic Francisella activate complement, but are resistant to complement-mediated lysis in part due to limited C3 deposition, rapid conversion of surface-bound C3b to C3bi, and the presence of LPS O Ag. PMID:18832715

Blood SC5b-9 complement levels increase at parturition during term and preterm labor.

PubMed

Segura-Cervantes, Enrique; Mancilla-Ramirez, Javier; Zurita, Luis; Paredes, Yuriria; Arredondo, José Luis; Galindo-Sevilla, Norma

2015-06-01

We explored the hypothesis that complement, an innate and adaptive immune effector, is active in the plasma of parturient women and is deposited on fetal membranes collected after delivery. A cross-sectional study was designed to evaluate complement activity at parturition. Pregnant women (n = 97) between 15 and 41 years of age were enrolled in a hospital protocol during the perinatal period to assess both SC5b-9 complement activity in blood and complement deposition on fetal membranes during parturition. Soluble SC5b-9 complement activity in plasma fractions was measured using a standard enzyme-linked immunosorbent assay (ELISA) that included specific anti-complement antibodies. Complement deposition on membranes was analyzed using immuno-dot blots and immunohistochemistry. Soluble SC5b-9 complement complex levels were increased in the plasma of women during term labor (TL; median 3361; range 1726-5670 ng/mL), preterm labor (PL; median 2958; range 1552-7092 ng/mL), and preterm premature rupture of membranes (PPROM; median 2272; range 167-6540 ng/mL) compared with pregnant women who were not in labor (P; median 1384; range 174-4570 ng/mL; P < 0.001, Kruskal-Wallis test). Active complement, as assessed by the C9 neo-antigen in C5b-9 complexes, was deposited on fetal membranes, with no difference between term and preterm delivery. The deposition of active complement on fetal membranes was confirmed by immunohistochemistry. Women who underwent non-labor-indicated Cesarean sections did not exhibit complement deposition. Soluble SC5b-9 complement complex levels increased in the plasma of women during parturition, and complement C5b-9 complexes were deposited on fetal membranes. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

Complement in Lupus Nephritis: New Perspectives.

PubMed

Bao, Lihua; Cunningham, Patrick N; Quigg, Richard J

2015-09-01

Systemic lupus erythematosus (SLE) is an autoimmune disorder caused by loss of tolerance to self-antigens, the production of autoantibodies and deposition of complement-fixing immune complexes (ICs) in injured tissues. SLE is characterized by a wide range of clinical manifestations and targeted organs, with lupus nephritis being one of the most serious complications. The complement system consists of three pathways and is tightly controlled by a set of regulatory proteins to prevent injudicious complement activation on host tissue. The involvement of the complement system in the pathogenesis of SLE is well accepted; yet, its exact role is still not clear. Complement plays dual roles in the pathogenesis of SLE. On the one hand, the complement system appears to have protective features in that hereditary homozygous deficiencies of classical pathway components, such as C1q and C4, are associated with an increased risk for SLE. On the other hand, IC-mediated activation of complement in affected tissues is clearly evident in both experimental and human SLE along with pathological features that are logical consequences of complement activation. Studies in genetically altered mice have shown that lack of complement inhibitors, such as complement factor H (CFH) or decay-accelerating factor (DAF) accelerates the development of experimental lupus nephritis, while treatment with recombinant protein inhibitors, such as Crry-Ig, CR2-Crry, CR2-DAF and CR2-CFH, ameliorates the disease development. Complement-targeted drugs, including soluble complement receptor 1 (TP10), C1 esterase inhibitor and a monoclonal anti-C5 antibody (eculizumab), have been shown to inhibit complement safely, and are now being investigated in a variety of clinical conditions. SLE is an autoimmune disorder which targets multiple systems. Complement is centrally involved and plays dual roles in the pathogenesis of SLE. Studies from experimental lupus models and clinical trials support the use of complement-targeted therapy in the treatment of SLE.

Cosmic Evolution Through UV Spectroscopy (CETUS): A NASA Probe-Class Mission Concept

NASA Astrophysics Data System (ADS)

Heap, Sara R.; CETUS Team

2017-01-01

CETUS is a probe-class mission concept proposed for study to NASA in November 2016. Its overarching objective is to provide access to the ultraviolet (~100-400 nm) after Hubble has died. CETUS will be a major player in the emerging global network of powerful, new telescopes such as E-ROSITA, DESI, Subaru/PFS, GMT, LSST, WFIRST, JWST, and SKA. The CETUS mission concept provisionally features a 1.5-m telescope with a suite of instruments including a near-UV multi-object spectrograph (200-400 nm) complementing Subaru/PFS observations, wide-field far-UV and near-UV cameras, and far-UV and near-UV spectrographs that can be operated in either high-resolution or low-resolution mode. We have derived the scope and specific science requirements for CETUS for understanding the evolutionary history of galaxies, stars, and dust, but other applications are possible.

Prediction of inflammatory responses induced by biomaterials in contact with human blood using protein fingerprint from plasma.

PubMed

Engberg, Anna E; Nilsson, Per H; Huang, Shan; Fromell, Karin; Hamad, Osama A; Mollnes, Tom Eirik; Rosengren-Holmberg, Jenny P; Sandholm, Kerstin; Teramura, Yuji; Nicholls, Ian A; Nilsson, Bo; Ekdahl, Kristina N

2015-01-01

Inappropriate complement activation is often responsible for incompatibility reactions that occur when biomaterials are used. Complement activation is therefore a criterion included in legislation regarding biomaterials testing. However, no consensus is yet available regarding appropriate complement-activation-related test parameters. We examined protein adsorption in plasma and complement activation/cytokine release in whole blood incubated with well-characterized polymers. Strong correlations were found between the ratio of C4 to its inhibitor C4BP and generation of 10 (mainly pro-inflammatory) cytokines, including IL-17, IFN-γ, and IL-6. The levels of complement activation products correlated weakly (C3a) or not at all (C5a, sC5b-9), confirming their poor predictive values. We have demonstrated a direct correlation between downstream biological effects and the proteins initially adhering to an artificial surface after contact with blood. Consequently, we propose the C4/C4BP ratio as a robust, predictor of biocompatibility with superior specificity and sensitivity over the current gold standard. Copyright © 2014 Elsevier Ltd. All rights reserved.

EOS-Aura's Ozone Monitoring Instrument (OMI): Validation Requirements

NASA Technical Reports Server (NTRS)

Brinksma, E. J.; McPeters, R.; deHaan, J. F.; Levelt, P. F.; Hilsenrath, E.; Bhartia, P. K.

2003-01-01

OMI is an advanced hyperspectral instrument that measures backscattered radiation in the UV and visible. It will be flown as part of the EOS Aura mission and provide data on atmospheric chemistry that is highly synergistic with other Aura instruments HIRDLS, MLS, and TES. OMI is designed to measure total ozone, aerosols, cloud information, and UV irradiances, continuing the TOMS series of global mapped products but with higher spatial resolution. In addition its hyperspectral capability enables measurements of trace gases such as SO2, NO2, HCHO, BrO, and OClO. A plan for validation of the various OM1 products is now being formulated. Validation of the total column and UVB products will rely heavily on existing networks of instruments, like NDSC. NASA and its European partners are planning aircraft missions for the validation of Aura instruments. New instruments and techniques (DOAS systems for example) will need to be developed, both ground and aircraft based. Lidar systems are needed for validation of the vertical distributions of ozone, aerosols, NO2 and possibly SO2. The validation emphasis will be on the retrieval of these products under polluted conditions. This is challenging because they often depend on the tropospheric profiles of the product in question, and because of large spatial variations in the troposphere. Most existing ground stations are located in, and equipped for, pristine environments. This is also true for almost all NDSC stations. OMI validation will need ground based sites in polluted environments and specially developed instruments, complementing the existing instrumentation.

Measuring Fracture Times Of Ceramics

NASA Technical Reports Server (NTRS)

Shlichta, Paul J.; Bister, Leo; Bickler, Donald G.

1989-01-01

Electrical measurements complement or replace fast cinematography. Electronic system measures microsecond time intervals between impacts of projectiles on ceramic tiles and fracture tiles. Used in research on ceramics and ceramic-based composite materials such as armor. Hardness and low density of ceramics enable them to disintegrate projectiles more efficiently than metals. Projectile approaches ceramic tile specimen. Penetrating foil squares of triggering device activate display and recording instruments. As ceramic and resistive film break oscilloscope plots increase in electrical resistance of film.

Complement System Part II: Role in Immunity

PubMed Central

Merle, Nicolas S.; Noe, Remi; Halbwachs-Mecarelli, Lise; Fremeaux-Bacchi, Veronique; Roumenina, Lubka T.

2015-01-01

The complement system has been considered for a long time as a simple lytic cascade, aimed to kill bacteria infecting the host organism. Nowadays, this vision has changed and it is well accepted that complement is a complex innate immune surveillance system, playing a key role in host homeostasis, inflammation, and in the defense against pathogens. This review discusses recent advances in the understanding of the role of complement in physiology and pathology. It starts with a description of complement contribution to the normal physiology (homeostasis) of a healthy organism, including the silent clearance of apoptotic cells and maintenance of cell survival. In pathology, complement can be a friend or a foe. It acts as a friend in the defense against pathogens, by inducing opsonization and a direct killing by C5b–9 membrane attack complex and by triggering inflammatory responses with the anaphylatoxins C3a and C5a. Opsonization plays also a major role in the mounting of an adaptive immune response, involving antigen presenting cells, T-, and B-lymphocytes. Nevertheless, it can be also an enemy, when pathogens hijack complement regulators to protect themselves from the immune system. Inadequate complement activation becomes a disease cause, as in atypical hemolytic uremic syndrome, C3 glomerulopathies, and systemic lupus erythematosus. Age-related macular degeneration and cancer will be described as examples showing that complement contributes to a large variety of conditions, far exceeding the classical examples of diseases associated with complement deficiencies. Finally, we discuss complement as a therapeutic target. PMID:26074922

Human L-ficolin, a recognition molecule of the lectin activation pathway of complement, activates complement by binding to pneumolysin, the major toxin of Streptococcus pneumoniae.

PubMed

Ali, Youssif M; Kenawy, Hany I; Muhammad, Adnan; Sim, Robert B; Andrew, Peter W; Schwaeble, Wilhelm J

2013-01-01

The complement system is an essential component of the immune response, providing a critical line of defense against different pathogens including S. pneumoniae. Complement is activated via three distinct pathways: the classical (CP), the alternative (AP) and the lectin pathway (LP). The role of Pneumolysin (PLY), a bacterial toxin released by S. pneumoniae, in triggering complement activation has been studied in vitro. Our results demonstrate that in both human and mouse sera complement was activated via the CP, initiated by direct binding of even non-specific IgM and IgG3 to PLY. Absence of CP activity in C1q(-/-) mouse serum completely abolished any C3 deposition. However, C1q depleted human serum strongly opsonized PLY through abundant deposition of C3 activation products, indicating that the LP may have a vital role in activating the human complement system on PLY. We identified that human L-ficolin is the critical LP recognition molecule that drives LP activation on PLY, while all of the murine LP recognition components fail to bind and activate complement on PLY. This work elucidates the detailed interactions between PLY and complement and shows for the first time a specific role of the LP in PLY-mediated complement activation in human serum.

Human L-ficolin, a Recognition Molecule of the Lectin Activation Pathway of Complement, Activates Complement by Binding to Pneumolysin, the Major Toxin of Streptococcus pneumoniae

PubMed Central

Ali, Youssif M.; Kenawy, Hany I.; Muhammad, Adnan; Sim, Robert B.

2013-01-01

The complement system is an essential component of the immune response, providing a critical line of defense against different pathogens including S. pneumoniae. Complement is activated via three distinct pathways: the classical (CP), the alternative (AP) and the lectin pathway (LP). The role of Pneumolysin (PLY), a bacterial toxin released by S. pneumoniae, in triggering complement activation has been studied in vitro. Our results demonstrate that in both human and mouse sera complement was activated via the CP, initiated by direct binding of even non-specific IgM and IgG3 to PLY. Absence of CP activity in C1q−/− mouse serum completely abolished any C3 deposition. However, C1q depleted human serum strongly opsonized PLY through abundant deposition of C3 activation products, indicating that the LP may have a vital role in activating the human complement system on PLY. We identified that human L-ficolin is the critical LP recognition molecule that drives LP activation on PLY, while all of the murine LP recognition components fail to bind and activate complement on PLY. This work elucidates the detailed interactions between PLY and complement and shows for the first time a specific role of the LP in PLY-mediated complement activation in human serum. PMID:24349316

Progress and trends in complement therapeutics.

PubMed

Ricklin, Daniel; Lambris, John D

2013-01-01

The past few years have proven to be a highly successful and exciting period for the field of complement-directed drug discovery and development. Driven by promising experiences with the first marketed complement drugs, increased knowledge about the involvement of complement in health and disease, and improvements in structural and analytical techniques as well as animal models of disease, the field has seen a surge in creative approaches to therapeutically intervene at various stages of the cascade. An impressive panel of compounds that show promise in clinical trials is meanwhile being lined up in the pipelines of both small biotechnology and big pharmaceutical companies. Yet with this new focus on complement-targeted therapeutics, important questions concerning target selection, point and length of intervention, safety, and drug delivery emerge. In view of the diversity of the clinical disorders involving abnormal complement activity or regulation, which include both acute and chronic diseases and affect a wide range of organs, diverse yet specifically tailored therapeutic approaches may be needed to shift complement back into balance. This chapter highlights the key changes in the field that shape our current perception of complement-targeted drugs and provides a brief overview of recent strategies and emerging trends. Selected examples of complement-related diseases and inhibitor classes are highlighted to illustrate the diversity and creativity in field.

Progress and Trends in Complement Therapeutics.

PubMed

Ricklin, Daniel; Lambris, John D

2013-01-01

The past few years have proven to be a highly successful and exciting period for the field of complement-directed drug discovery and development. Driven by promising experiences with the first marketed complement drugs, increased knowledge about the involvement of complement in health and disease, and improvements in structural and analytical techniques as well as animal models of disease, the field has seen a surge in creative approaches to therapeutically intervene at various stages of the cascade. An impressive panel of compounds that show promise in clinical trials is meanwhile being lined up in the pipelines of both small biotechnology and big pharmaceutical companies. Yet with this new focus on complement-targeted therapeutics, important questions concerning target selection, point and length of intervention, safety, and drug delivery emerge. In view of the diversity of the clinical disorders involving abnormal complement activity or regulation, which include both acute and chronic diseases and affect a wide range of organs, diverse yet specifically tailored therapeutic approaches may be needed to shift complement back into balance. This chapter highlights the key changes in the field that shape our current perception of complement-targeted drugs and provides a brief overview of recent strategies and emerging trends. Selected examples of complement-related diseases and inhibitor classes are highlighted to illustrate the diversity and creativity in field.

SALO, a novel classical pathway complement inhibitor from saliva of the sand fly Lutzomyia longipalpis

PubMed Central

Ferreira, Viviana P.; Fazito Vale, Vladimir; Pangburn, Michael K.; Abdeladhim, Maha; Ferreira Mendes-Sousa, Antonio; Coutinho-Abreu, Iliano V.; Rasouli, Manoochehr; Brandt, Elizabeth A.; Meneses, Claudio; Lima, Kolyvan Ferreira; Nascimento Araújo, Ricardo; Horácio Pereira, Marcos; Kotsyfakis, Michalis; Oliveira, Fabiano; Kamhawi, Shaden; Ribeiro, Jose M. C.; Gontijo, Nelder F.; Collin, Nicolas; Valenzuela, Jesus G.

2016-01-01

Blood-feeding insects inject potent salivary components including complement inhibitors into their host’s skin to acquire a blood meal. Sand fly saliva was shown to inhibit the classical pathway of complement; however, the molecular identity of the inhibitor remains unknown. Here, we identified SALO as the classical pathway complement inhibitor. SALO, an 11 kDa protein, has no homology to proteins of any other organism apart from New World sand flies. rSALO anti-complement activity has the same chromatographic properties as the Lu. longipalpis salivary gland homogenate (SGH)counterparts and anti-rSALO antibodies blocked the classical pathway complement activity of rSALO and SGH. Both rSALO and SGH inhibited C4b deposition and cleavage of C4. rSALO, however, did not inhibit the protease activity of C1s nor the enzymatic activity of factor Xa, uPA, thrombin, kallikrein, trypsin and plasmin. Importantly, rSALO did not inhibit the alternative or the lectin pathway of complement. In conclusion our data shows that SALO is a specific classical pathway complement inhibitor present in the saliva of Lu. longipalpis. Importantly, due to its small size and specificity, SALO may offer a therapeutic alternative for complement classical pathway-mediated pathogenic effects in human diseases. PMID:26758086

Demons registration for in vivo and deformable laser scanning confocal endomicroscopy.

PubMed

Chiew, Wei-Ming; Lin, Feng; Seah, Hock Soon

2017-09-01

A critical effect found in noninvasive in vivo endomicroscopic imaging modalities is image distortions due to sporadic movement exhibited by living organisms. In three-dimensional confocal imaging, this effect results in a dataset that is tilted across deeper slices. Apart from that, the sequential flow of the imaging-processing pipeline restricts real-time adjustments due to the unavailability of information obtainable only from subsequent stages. To solve these problems, we propose an approach to render Demons-registered datasets as they are being captured, focusing on the coupling between registration and visualization. To improve the acquisition process, we also propose a real-time visual analytics tool, which complements the imaging pipeline and the Demons registration pipeline with useful visual indicators to provide real-time feedback for immediate adjustments. We highlight the problem of deformation within the visualization pipeline for object-ordered and image-ordered rendering. Visualizations of critical information including registration forces and partial renderings of the captured data are also presented in the analytics system. We demonstrate the advantages of the algorithmic design through experimental results with both synthetically deformed datasets and actual in vivo, time-lapse tissue datasets expressing natural deformations. Remarkably, this algorithm design is for embedded implementation in intelligent biomedical imaging instrumentation with customizable circuitry. (2017) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE).

Metabolomics by Gas Chromatography-Mass Spectrometry: the combination of targeted and untargeted profiling

PubMed Central

Fiehn, Oliver

2016-01-01

Gas chromatography-mass spectrometry (GC-MS)-based metabolomics is ideal for identifying and quantitating small molecular metabolites (<650 daltons), including small acids, alcohols, hydroxyl acids, amino acids, sugars, fatty acids, sterols, catecholamines, drugs, and toxins, often using chemical derivatization to make these compounds volatile enough for gas chromatography. This unit shows that on GC-MS- based metabolomics easily allows integrating targeted assays for absolute quantification of specific metabolites with untargeted metabolomics to discover novel compounds. Complemented by database annotations using large spectral libraries and validated, standardized standard operating procedures, GC-MS can identify and semi-quantify over 200 compounds per study in human body fluids (e.g., plasma, urine or stool) samples. Deconvolution software enables detection of more than 300 additional unidentified signals that can be annotated through accurate mass instruments with appropriate data processing workflows, similar to liquid chromatography-MS untargeted profiling (LC-MS). Hence, GC-MS is a mature technology that not only uses classic detectors (‘quadrupole’) but also target mass spectrometers (‘triple quadrupole’) and accurate mass instruments (‘quadrupole-time of flight’). This unit covers the following aspects of GC-MS-based metabolomics: (i) sample preparation from mammalian samples, (ii) acquisition of data, (iii) quality control, and (iv) data processing. PMID:27038389

Limiting the consumption of sugar sweetened beverages in Mexico's obesogenic environment: a qualitative policy review and stakeholder analysis.

PubMed

Moise, Nathalie; Cifuentes, Enrique; Orozco, Emanuel; Willett, Walter

2011-11-01

Mexico is building a legal framework to address its childhood obesity epidemic. Sugar sweetened beverages (SSB) in the school environment represent a major policy challenge. We addressed the following questions: What barriers inhibit political attention to SSB and childhood obesity? What political instruments, international and national, exist to guide agenda setting in Mexico? What opportunities exist for policy adoption? We conducted a systematic review of international and national legal instruments concerned with SSB consumption. We traced process, conducting interviews with key informants. Thematic analysis helped us identify barriers and opportunities for public health interventions. We found 11 national policy instruments, but detected implementation gaps and weak fiscal policies on SSB consumption in schools: limited drinking water infrastructure, SSB industry interests, and regulatory ambiguities addressing reduction of sugar in beverages. Public policy should target marketing practices and taxation. The school environment remains a promising target for policy. Access to safe drinking water must complement comprehensive and multi-sector policy approaches to reduce access to SSB.

The Magnetosphere Imager Mission Concept Definition Study

NASA Technical Reports Server (NTRS)

Johnson, L.; Herrmann, M.; Alexander, Reggie; Beabout, Brent; Blevins, Harold; Bridge, Scott; Burruss, Glenda; Buzbee, Tom; Carrington, Connie; Chandler, Holly;

Optical 1's and 2's complement devices using lithium-niobate-based waveguide

NASA Astrophysics Data System (ADS)

Pal, Amrindra; Kumar, Santosh; Sharma, Sandeep

2016-12-01

Optical 1's and 2's complement devices are proposed with the help of lithium-niobate-based Mach-Zehnder interferometers. It has a powerful capability of switching an optical signal from one port to the other port with the help of an electrical control signal. The paper includes the optical conversion scheme using sets of optical switches. 2's complement is common in computer systems and is used in binary subtraction and logical manipulation. The operation of the circuits is studied theoretically and analyzed through numerical simulations. The truth table of these complement methods is verified with the beam propagation method and MATLAB® simulation results.

Active/passive scanning. [airborne multispectral laser scanners for agricultural and water resources applications

NASA Technical Reports Server (NTRS)

Woodfill, J. R.; Thomson, F. J.

1979-01-01

The paper deals with the design, construction, and applications of an active/passive multispectral scanner combining lasers with conventional passive remote sensors. An application investigation was first undertaken to identify remote sensing applications where active/passive scanners (APS) would provide improvement over current means. Calibration techniques and instrument sensitivity are evaluated to provide predictions of the APS's capability to meet user needs. A preliminary instrument design was developed from the initial conceptual scheme. A design review settled the issues of worthwhile applications, calibration approach, hardware design, and laser complement. Next, a detailed mechanical design was drafted and construction of the APS commenced. The completed APS was tested and calibrated in the laboratory, then installed in a C-47 aircraft and ground tested. Several flight tests completed the test program.

Ultraviolet spectrophotometry of comet Giacobini-Zinner during the ICE encounter. [International Cometary Explorer (ICE)

NASA Technical Reports Server (NTRS)

Ahearn, Michael F.; Mcfadden, Lucy A.; Feldman, Paul D.; Boehnhardt, Hermann; Rahe, Juergen; Festou, Michael; Brandt, John C.; Maran, Stephen P.; Niedner, Malcom B.; Smith, Andrew M.

1986-01-01

The IUE spectrophotometry of Comet P/Giacobini-Zinner was acquired in support of the International Cometary Explorer (ICE) mission. The abundances (or upper limits) of UV-active species were calculated. During the ICE encounter the H2O production rate was 3 times 10 to the 28th power/sec, + or - 50%, consistent with values derived from the ICE experiments. Comparison of the abundance of CO2(+) ions with the total electron density measured by the plasma electron experiment on ICE indicates a deficiency of ions relative to electrons indicating a population of ions not detected by remote sensing. The absence of detectable Mg(+) rules out this species as a possible ion of M/Q = 24 detected by the Ion Composition Instrument, part of the ICE complement of instruments.

A tangentially viewing fast ion D-alpha diagnostic for NSTX.

PubMed

Bortolon, A; Heidbrink, W W; Podestà, M

2010-10-01

A second fast ion D-alpha (FIDA) installation is planned at NSTX to complement the present perpendicular viewing FIDA diagnostics. Following the present diagnostic scheme, the new diagnostic will consist of two instruments: a spectroscopic diagnostic that measures fast ion spectra and profiles at 16 radial points with 5-10 ms resolution and a system that uses a band pass filter and photomultiplier to measure changes in FIDA light with 50 kHz sampling rate. The new pair of FIDA instruments will view the heating beams tangentially. The viewing geometry minimizes spectral contamination by beam emission or edge sources of background emission. The improved velocity-space resolution will provide detailed information about neutral-beam current drive and about fast ion acceleration and transport by injected radio frequency waves and plasma instabilities.

Potential influences of complement factor H in autoimmune inflammatory and thrombotic disorders.

PubMed

Ferluga, Janez; Kouser, Lubna; Murugaiah, Valarmathy; Sim, Robert B; Kishore, Uday

2017-04-01

Complement system homeostasis is important for host self-protection and anti-microbial immune surveillance, and recent research indicates roles in tissue development and remodelling. Complement also appears to have several points of interaction with the blood coagulation system. Deficiency and altered function due to gene mutations and polymorphisms in complement effectors and regulators, including Factor H, have been associated with familial and sporadic autoimmune inflammatory - thrombotic disorders, in which autoantibodies play a part. These include systemic lupus erythematosus, rheumatoid arthritis, atypical haemolytic uremic syndrome, anti-phospholipid syndrome and age-related macular degeneration. Such diseases are generally complex - multigenic and heterogeneous in their symptoms and predisposition/susceptibility. They usually need to be triggered by vascular trauma, drugs or infection and non-complement genetic factors also play a part. Underlying events seem to include decline in peripheral regulatory T cells, dendritic cell, and B cell tolerance, associated with alterations in lymphoid organ microenvironment. Factor H is an abundant protein, synthesised in many cell types, and its reported binding to many different ligands, even if not of high affinity, may influence a large number of molecular interactions, together with the accepted role of Factor H within the complement system. Factor H is involved in mesenchymal stem cell mediated tolerance and also contributes to self-tolerance by augmenting iC3b production and opsonisation of apoptotic cells for their silent dendritic cell engulfment via complement receptor CR3, which mediates anti-inflammatory-tolerogenic effects in the apoptotic cell context. There may be co-operation with other phagocytic receptors, such as complement C1q receptors, and the Tim glycoprotein family, which specifically bind phosphatidylserine expressed on the apoptotic cell surface. Factor H is able to discriminate between self and nonself surfaces for self-protection and anti-microbe defence. Factor H, particularly as an abundant platelet protein, may also modulate blood coagulation, having an anti-thrombotic role. Here, we review a number of interaction pathways in coagulation and in immunity, together with associated diseases, and indicate where Factor H may be expected to exert an influence, based on reports of the diversity of ligands for Factor H. Copyright © 2017 Elsevier Ltd. All rights reserved.

University of Wisconsin Cirrus Remote Sensing Pilot Experiment

NASA Technical Reports Server (NTRS)

Ackerman, Steven A.; Eloranta, Ed W.; Grund, Chris J.; Knuteson, Robert O.; Revercomb, Henry E.; Smith, William L.; Wylie, Donald P.

1993-01-01

During the period of 26 October 1989 through 6 December 1989 a unique complement of measurements was made at the University of Wisconsin-Madison to study the radiative properties of cirrus clouds. Simultaneous observations were obtained from a scanning lidar, two interferometers, a high spectral resolution lidar, geostationary and polar orbiting satellites, radiosonde launches, and a whole-sky imager. This paper describes the experiment, the instruments deployed, and, as an example, the data collected during one day of the experiment.

The polarimeter and the multispectral radiometer as remote probes of aerosols

NASA Technical Reports Server (NTRS)

Kuriyan, J. G.

1975-01-01

Attempts made to establish the viability of the polarimeter as a ground based remote probe of atmospheric aerosols under varying meteorological conditions are reported. Extension radiometer data are also given and a comparison was made with polarimeter data to determine if one could complement/supplement the other. The analyses show that the two instruments are complementary devices and any information obtained from one will greatly facilitate the analysis of the other. Detailed results are given in graphical form.

Stratospheric Observatory For Infrared Astronomy (SOFIA)

NASA Astrophysics Data System (ADS)

Becklin, E. E.; Moon, L. J.

2004-12-01

The joint U.S. and German SOFIA project to develop and operate a 2.5-meter infrared airborne telescope in a Boeing 747-SP is now well into development. First science flights will begin in 2004 with 20% of the observing time assigned to German investigators. The observatory is expected to operate for over 20 years. The sensitivity, characteristics and science instrument complement are discussed. SOFIA will have a number of experiments related to Dust Debris Disks; some of these are discussed.

Development of exercise devices to minimize musculoskeletal and cardiovascular deconditioning in microgravity

NASA Technical Reports Server (NTRS)

Schwandt, Douglas F.; Whalen, Robert T.; Watenpaugh, Donald E.; Parazynski, Scott E.; Hargens, Alan R.

1991-01-01

The paper describes three exercise devices, developed at the NASA-Ames Research Center, for maintaining musculoskeletal and cardiovascular fitness in astronauts during extended space flights. These devices represent the following exercise concepts: (1) exercise against LBNP, (2) instrumented dynamic interlimb resistance, and (3) multiple resistive exercise. The three devices complement each other to provide the aerobic and strength training exercises for different situations. All three devices permit eccentric, concentric, and isometric contractions for a variety of exercises.

STS-37 Gamma Ray Observatory (GRO) grappled by RMS

NASA Image and Video Library

1991-04-07

Backdropped against the Earth's surface, the Gamma Ray Observatory (GRO) with its solar array (SA) panels deployed is grappled by the remote manipulator system (RMS) during STS-37 systems checkout. GRO's four complement instruments are visible: the Energetic Gamma Ray Experiment Telescope (EGRET) (at the bottom); the Imaging Compton Telescope (COMPTEL) (center); the Oriented Scintillation Spectrometer Experiment (OSSE) (top); and Burst and Transient Source Experiment (BATSE) (on four corners). The view was taken by STS-37 crew through an aft flight deck overhead window.

Kupffer cell complement receptor clearance function and host defense.

PubMed

Loegering, D J

1986-01-01

Kupffer cells are well known to be important for normal host defense function. The development of methods to evaluate the in vivo function of specific receptors on Kupffer cells has made it possible to assess the role of these receptors in host defense. The rationale for studying complement receptors is based on the proposed important role of these receptors in host defense and on the observation that the hereditary deficiency of a complement receptor is associated with recurrent severe bacterial infections. The studies reviewed here demonstrate that forms of injury that are associated with depressed host defense including thermal injury, hemorrhagic shock, trauma, and surgery also cause a decrease in complement receptor clearance function. This decrease in Kupffer cell receptor clearance function was shown not to be the result of depressed hepatic blood flow or depletion of complement components. Complement receptor function was also depressed following the phagocytosis of particulates that are known to depress Kupffer cell host defense function. Endotoxemia and bacteremia also were associated with a depression of complement receptor function. Complement receptor function was experimentally depressed in uninjured animals by the phagocytosis of IgG-coated erythrocytes. There was a close association between the depression of complement receptor clearance function and increased susceptibility to the lethal effects of endotoxin and bacterial infection. These studies support the hypotheses that complement receptors on Kupffer cells are important for normal host defense and that depression of the function of these receptors impairs host defense.

Detection of pigments of halophilic endoliths from gypsum: Raman portable instrument and European Space Agency's prototype analysis.

PubMed

Culka, Adam; Osterrothová, Kateřina; Hutchinson, Ian; Ingley, Richard; McHugh, Melissa; Oren, Aharon; Edwards, Howell G M; Jehlička, Jan

2014-12-13

A prototype instrument, under development at the University of Leicester, for the future European Space Agency (ESA) ExoMars mission, was used for the analysis of microbial pigments within a stratified gypsum crust from a hypersaline saltern evaporation pond at Eilat (Israel). Additionally, the same samples were analysed using a miniaturized Raman spectrometer, featuring the same 532 nm excitation. The differences in the position of the specific bands, attributed to carotenoid pigments from different coloured layers, were minor when analysed by the ESA prototype instrument; therefore, making it difficult to distinguish among the different pigments. The portable Delta Nu Advantage instrument allowed for the discrimination of microbial carotenoids from the orange/green and purple layers. The purpose of this study was to complement previous laboratory results with new data and experience with portable or handheld Raman systems, even with a dedicated prototype Raman system for the exploration of Mars. The latter is equipped with an excitation wavelength falling within the carotenoid polyene resonance region. The ESA prototype Raman instrument detected the carotenoid pigments (biomarkers) with ease, although further detailed distinctions among them were not achieved. © 2014 The Author(s) Published by the Royal Society. All rights reserved.

Core Facility of the Juelich Observatory for Cloud Evolution (JOYCE - CF)

NASA Astrophysics Data System (ADS)

Beer, J.; Troemel, S.

2017-12-01

A multiple and holistic multi-sensor monitoring of clouds and precipitation processes is a challenging but promising task in the meteorological community. Instrument synergies offer detailed views in microphysical and dynamical developments of clouds. Since 2017 The the Juelich Observatory for Cloud Evolution (JOYCE) is transformed into a Core Facility (JOYCE - CF). JOYCE - CF offers multiple long-term remote sensing observations of the atmosphere, develops an easy access to all observations and invites scientists word wide to exploit the existing data base for their research but also to complement JOYCE-CF with additional long-term or campaign instrumentation. The major instrumentation contains a twin set of two polarimetric X-band radars, a microwave profiler, two cloud radars, an infrared spectrometer, a Doppler lidar and two ceilometers. JOYCE - CF offers easy and open access to database and high quality calibrated observations of all instruments. E.g. the two polarimetric X-band radars which are located in 50 km distance are calibrated using the self-consistency method, frequently repeated vertical pointing measurements as well as instrument synergy with co-located micro-rain radar and distrometer measurements. The presentation gives insights into calibration procedures, the standardized operation procedures and recent synergistic research exploiting our radars operating at three different frequencies.

The Evolution of Spacelab Ultraviolet Astronomy Missions from OSS-3 through -7 to Astro-1

NASA Astrophysics Data System (ADS)

Gull, Theodore

2018-01-01

In the 1960s and 1970s, NASA was building towards a robust program in space astronomy. An evolutionary step from ground-based astronomy to space astronomy was human operation of space telescopes as astronomy in general evolved from astronomers directly at the telescope to application of computers and long distance communications to control to operate remote telescopes. Today ground-based telescopes and space observatories from cubesats to the Hubble Space Telescope and soon the James Webb Space Telescope are routinely operated remotely.In response to the Spacelab Announcement of Opportunity in the early 1980s, three ultraviolet experiments – the Hopkins Ultraviolet Telescope, the Ultraviolet Imaging Telescope and the Wisconsin Ultraviolet PhotoPolarimetry Experiment -- all instruments derived from multiple sounding rocket flights--were selected to fly as an integrated payload attached to a space shuttle. The justification for professional astronomers, both as Mission Specialists from the astronaut cadre and Payload Specialists from the instrument teams, was built to ensure key technical skills both of the science and the instruments. Bundled together as OSS-3 through -7 flights focused on Comet Halley, the experiments went through many changes and delays as a pathfinder for an anticipated series of attached astronomy payloads.By 1986, the five-flight mission had evolved into two missions, Astro-1 dedicated primarily to observe Halley’s Comet in early March 1986 and Astro-2 to fly about one year later. Due to the Challenger disaster 35 days before scheduled launch of Astro-1, the mission went through an initial cancellation and then re-scheduling once the instrument complement of Astro-1 was expanded to include Broad Band X-ray Telescope with emphasis on studying SN1987A. Ultimately Astro-1 flew in December 1990 partnered with an X-ray experiment focused on SN1987A.The nine-day mission was mostly successful despite multiple technical issues overcome by the NASA and instrument teams. Dozens of refereed papers results and five years later, Astro-2, with the three ultraviolet instruments accomplished a seventeen-day mission.

Measurement of change in health status with Rasch models.

PubMed

Anselmi, Pasquale; Vidotto, Giulio; Bettinardi, Ornella; Bertolotti, Giorgio

2015-02-07

The traditional approach to the measurement of change presents important drawbacks (no information at individual level, ordinal scores, variance of the measurement instrument across time points), which Rasch models overcome. The article aims to illustrate the features of the measurement of change with Rasch models. To illustrate the measurement of change using Rasch models, the quantitative data of a longitudinal study of heart-surgery patients (N = 98) were used. The scale "Perception of Positive Change" was used as an example of measurement instrument. All patients underwent cardiac rehabilitation, individual psychological intervention, and educational intervention. Nineteen patients also attended progressive muscle relaxation group trainings. The scale was administered before and after the interventions. Three Rasch approaches were used. Two separate analyses were run on the data from the two time points to test the invariance of the instrument. An analysis was run on the stacked data from both time points to measure change in a common frame of reference. Results of the latter analysis were compared with those of an analysis that removed the influence of local dependency on patient measures. Statistics t, χ(2) and F were used for comparing the patient and item measures estimated in the Rasch analyses (a-priori α = .05). Infit, Outfit, R and item Strata were used for investigating Rasch model fit, reliability, and validity of the instrument. Data of all 98 patients were included in the analyses. The instrument was reliable, valid, and substantively unidimensional (Infit, Outfit < 2 for all items, R = .84, item Strata range = 3.93-6.07). Changes in the functioning of the instrument occurred across the two time, which prevented the use of the two separate analyses to unambiguously measure change. Local dependency had a negligible effect on patient measures (p ≥ .8674). Thirteen patients improved, whereas 3 worsened. The patients who attended the relaxation group trainings did not report greater improvement than those who did not (p = .1007). Rasch models represent a valid framework for the measurement of change and a useful complement to traditional approaches.

Method and apparatus for ion mobility spectrometry with alignment of dipole direction (IMS-ADD)

DOEpatents

Shvartsburg, Alexandre A [Richland, WA; Tang, Keqi [Richland, WA; Smith, Richard D [Richland, WA

2007-01-30

Techniques and instrumentation are described for analyses of substances, including complex samples/mixtures that require separation prior to characterization of individual components. A method is disclosed for separation of ion mixtures and identification of ions, including protein and other macromolecular ions and their different structural isomers. Analyte ions are not free to rotate during the separation, but are substantially oriented with respect to the drift direction. Alignment is achieved by applying, at a particular angle to the drift field, a much stronger alternating electric field that "locks" the ion dipoles with moments exceeding a certain value. That value depends on the buffer gas composition, pressure, and temperature, but may be as low as .about.3 Debye under certain conditions. The presently disclosed method measures the direction-specific cross-sections that provide the structural information complementing that obtained from known methods, and, when coupled to those methods, increases the total peak capacity and specificity of gas-phase separations. Simultaneous 2-D separations by direction-specific cross sections along and orthogonally to the ion dipole direction are also possible.

Optical Diagnostic System for Solar Sails: Phase 1 Final Report

NASA Technical Reports Server (NTRS)

Pappa, Richard S.; Blandino, Joseph R.; Caldwell, Douglas W.; Carroll, Joseph A.; Jenkins, Christopher H. M.; Pollock, Thomas C.

2004-01-01

NASA's In-Space Propulsion program recently selected AEC-ABLE Engineering and L'Garde, Inc. to develop scale-model solar sail hardware and demonstrate its functionality on the ground. Both are square sail designs with lightweight diagonal booms (<100 g/m) and ultra-thin membranes (<10 g/sq m). To support this technology, the authors are developing an integrated diagnostics instrumentation package for monitoring solar sail structures such as these in a near-term flight experiment. We refer to this activity as the "Optical Diagnostic System (ODS) for Solar Sails" project. The approach uses lightweight optics and photogrammetric techniques to measure solar sail membrane and boom shape and dynamics, thermography to map temperature, and non-optical sensors including MEMS accelerometers and load cells. The diagnostics package must measure key structural characteristics including deployment dynamics, sail support tension, boom and sail deflection, boom and sail natural frequencies, sail temperature, and sail integrity. This report summarizes work in the initial 6-month Phase I period (conceptual design phase) and complements the final presentation given in Huntsville, AL on January 14, 2004.

Quiescent complement in nonhuman primates during E coli Shiga toxin-induced hemolytic uremic syndrome and thrombotic microangiopathy.

PubMed

Lee, Benjamin C; Mayer, Chad L; Leibowitz, Caitlin S; Stearns-Kurosawa, D J; Kurosawa, Shinichiro

2013-08-01

Enterohemorrhagic Escherichia coli (EHEC) produce ribosome-inactivating Shiga toxins (Stx1, Stx2) responsible for development of hemolytic uremic syndrome (HUS) and acute kidney injury (AKI). Some patients show complement activation during EHEC infection, raising the possibility of therapeutic targeting of complement for relief. Our juvenile nonhuman primate (Papio baboons) models of endotoxin-free Stx challenge exhibit full spectrum HUS, including thrombocytopenia, hemolytic anemia, and AKI with glomerular thrombotic microangiopathy. There were no significant increases in soluble terminal complement complex (C5b-9) levels after challenge with lethal Stx1 (n = 6) or Stx2 (n = 5) in plasma samples from T0 to euthanasia at 49.5 to 128 hours post-challenge. d-dimer and cell injury markers (HMGB1, histones) confirmed coagulopathy and cell injury. Thus, complement activation is not required for the development of thrombotic microangiopathy and HUS induced by EHEC Shiga toxins in these preclinical models, and benefits or risks of complement inhibition should be studied further for this infection.

MODIS Snow-Cover Products

NASA Technical Reports Server (NTRS)

Hall, Dorothy K.; Riggs, George A.; Salomonson, Vinvent V.; DiGirolamo, Nicolo; Bayr, Klaus J.; Houser, Paul (Technical Monitor)

2001-01-01

On December 18, 1999, the Terra satellite was launched with a complement of five instruments including the Moderate Resolution Imaging Spectroradiometer (MODIS). Many geophysical products are derived from MODIS data including global snow-cover products. These products have been available through the National Snow and Ice Data Center (NSIDC) Distributed Active Archive Center (DAAC) since September 13, 2000. MODIS snow-cover products represent potential improvement to the currently available operation products mainly because the MODIS products are global and 500-m resolution, and have the capability to separate most snow and clouds. Also the snow-mapping algorithms are automated which means that a consistent data set is generated for long-term climates studies that require snow-cover information. Extensive quality assurance (QA) information is stored with the product. The snow product suite starts with a 500-m resolution swath snow-cover map which is gridded to the Integerized Sinusoidal Grid to produce daily and eight-day composite tile products. The sequence then proceeds to a climate-modeling grid product at 5-km spatial resolution, with both daily and eight-day composite products. A case study from March 6, 2000, involving MODIS data and field and aircraft measurements, is presented. Near-term enhancements include daily snow albedo and fractional snow cover.

IR Spectrometer Using 90-Degree Off-Axis Parabolic Mirrors

DOE Office of Scientific and Technical Information (OSTI.GOV)

Robert M. Malone, Ian J. McKenna

2008-03-01

A gated spectrometer has been designed for real-time, pulsed infrared (IR) studies at the National Synchrotron Light Source at the Brookhaven National Laboratory. A pair of 90-degree, off-axis parabolic mirrors are used to relay the light from an entrance slit to an output recording camera. With an initial wavelength range of 1500–4500 nm required, gratings could not be used in the spectrometer because grating orders would overlap. A magnesium oxide prism, placed between these parabolic mirrors, serves as the dispersion element. The spectrometer is doubly telecentric. With proper choice of the air spacing between the prism and the second parabolicmore » mirror, any spectral region of interest within the InSb camera array’s sensitivity region can be recorded. The wavelengths leaving the second parabolic mirror are collimated, thereby relaxing the camera positioning tolerance. To set up the instrument, two different wavelength (visible) lasers are introduced at the entrance slit and made collinear with the optical axis via flip mirrors. After dispersion by the prism, these two laser beams are directed to tick marks located on the outside housing of the gated IR camera. This provides first-order wavelength calibration for the instrument. Light that is reflected off the front prism face is coupled into a high-speed detector to verify steady radiance during the gated spectral imaging. Alignment features include tick marks on the prism and parabolic mirrors. This instrument was designed to complement single-point pyrometry, which provides continuous time histories of a small collection of spots from shock-heated targets.« less

IR Spectrometer Using 90-degree Off-axis Parabolic Mirrors

DOE Office of Scientific and Technical Information (OSTI.GOV)

Robert M. Malone, Richard, G. Hacking, Ian J. McKenna, and Daniel H. Dolan

2008-09-02

A gated spectrometer has been designed for real-time, pulsed infrared (IR) studies at the National Synchrotron Light ource at the Brookhaven National Laboratory. A pair of 90-degree, off-axis parabolic mirrors are used to relay the light from an entrance slit to an output IR recording camera. With an initial wavelength range of 1500–4500 nm required, gratings could not be used in the spectrometer because grating orders would overlap. A magnesium oxide prism, placed between these parabolic mirrors, serves as the dispersion element. The spectrometer is doubly telecentric. With proper choice of the air spacing between the prism and the secondmore » parabolic mirror, any spectral region of interest within the InSb camera array’s sensitivity region can be recorded. The wavelengths leaving the second parabolic mirror are collimated, thereby relaxing the camera positioning tolerance. To set up the instrument, two different wavelength (visible) lasers are introduced at the entrance slit and made collinear with the optical axis via flip mirrors. After dispersion by the prism, these two laser beams are directed to tick marks located on the outside housing of the gated IR camera. This provides first-order wavelength calibration for the instrument. Light that is reflected off the front prism face is coupled into a high-speed detector to verify steady radiance during the gated spectral imaging. Alignment features include tick marks on the prism and parabolic mirrors. This instrument was designed to complement singlepoint pyrometry, which provides continuous time histories of a small collection of spots from shock-heated targets.« less

Targeting the complement system for the management of retinal inflammatory and degenerative diseases.

PubMed

Xu, Heping; Chen, Mei

2016-09-15

The retina, an immune privileged tissue, has specialized immune defense mechanisms against noxious insults that may exist in diseases such as age-related macular degeneration (AMD), diabetic retinopathy (DR), uveoretinitis and glaucoma. The defense system consists of retinal innate immune cells (including microglia, perivascular macrophages, and a small population of dendritic cells) and the complement system. Under normal aging conditions, retinal innate immune cells and the complement system undergo a low-grade activation (parainflammation) which is important for retinal homeostasis. In disease states such as AMD and DR, the parainflammatory response is dysregulated and develops into detrimental chronic inflammation. Complement activation in the retina is an important part of chronic inflammation and may contribute to retinal pathology in these disease states. Here, we review the evidence that supports the role of uncontrolled or dysregulated complement activation in various retinal degenerative and angiogenic conditions. We also discuss current strategies that are used to develop complement-based therapies for retinal diseases such as AMD. The potential benefits of complement inhibition in DR, uveoretinitis and glaucoma are also discussed, as well as the need for further research to better understand the mechanisms of complement-mediated retinal damage in these disease states. Copyright © 2016 The Authors. Published by Elsevier B.V. All rights reserved.

[Medical care unit -- a suitable instrument for ambulatory patient-adequate care and performance-related remuneration].

PubMed

Rudolph, P; Isensee, D; Gerlach, E; Gross, H

2013-02-01

The question of whether a medical care unit is an appropriate tool for outpatient care has been discussed for a long time. Our aim is to investigate whether the MCU is an effective instrument for outpatient care and adequate performance-related remuneration. This retro- and prospective overview of the work included statements on legal foundations for medical care units, for reimbursement of services in medical care units, the development of medical care centres in Germany and a listing of the specific advantages and disadvantages of an MCU. This article focuses on the generally applicable facts and complements them with examples from general, visceral and vascular surgery. The main quantitative data on medical centre statistics come from different publications of the National Association of Statutory Health Insurance for Physicians. From a legal point of view the instrument MCU allows the participating of ambulatory and stationary care in the framework of medical care contracts. This has been especially extended for stationary applications, including the spectrum of possibilities that can contribute under certain circumstances for the provision of medical care in underdeveloped regions. Freelancers can benefit primarily from financial risk and minimising bureaucratic routine. The remuneration for services performed in the MCU is analogous to that of other ambulatory care providers. Basically, there are no disadvantages, but a greater design freedom and opportunities for the generation of aggregates are visible. The number of MCU in Germany has quadrupled in the last five years, indicating an establishment of an outpatient care landscape. MCU offers from the patient's perspective, providers and policy specific advantages and disadvantages. Indeed the benefits outweigh the disadvantages, but this is not yet verified by qualitative studies. The question of the appropriateness of medical care units as outpatient care instrumentation must be considered differentially. Under current conditions it appears suitable for ensuring the MCU and the supplement of care supply. Whether a value can be generated in the quality of care of patients, however, has to be examined separately, as there are no valid data so far. The same applies to economic assessments of costs and benefits from an economic perspective. © Georg Thieme Verlag KG Stuttgart · New York.

Innovative Use of the Law to Address Complex Global Health Problems Comment on "The Legal Strength of International Health Instruments - What It Brings toGlobal Health Governance?"

PubMed

Walls, Helen L; Ooms, Gorik

2017-05-20

Addressing the increasingly globalised determinants of many important problems affecting human health is a complex task requiring collective action. We suggest that part of the solution to addressing intractable global health issues indeed lies with the role of new legal instruments in the form of globally binding treaties, as described in the recent article of Nikogosian and Kickbusch. However, in addition to the use of international law to develop new treaties, another part of the solution may lie in innovative use of existing legal instruments. A 2015 court ruling in The Hague, which ordered the Dutch government to cut greenhouse gas emissions by at least 25% within five years, complements this perspective, suggesting a way forward for addressing global health problems that critically involves civil society and innovative use of existing domestic legal instruments. © 2017 The Author(s); Published by Kerman University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

The Dark Energy Spectroscopic Instrument (DESI)

NASA Astrophysics Data System (ADS)

Flaugher, Brenna; Bebek, Chris

2014-07-01

The Dark Energy Spectroscopic Instrument (DESI) is a Stage IV ground-based dark energy experiment that will study baryon acoustic oscillations (BAO) and the growth of structure through redshift-space distortions with a wide-area galaxy and quasar spectroscopic redshift survey. The DESI instrument consists of a new wide-field (3.2 deg. linear field of view) corrector plus a multi-object spectrometer with up to 5000 robotically positioned optical fibers and will be installed at prime focus on the Mayall 4m telescope at Kitt Peak, Arizona. The fibers feed 10 three-arm spectrographs producing spectra that cover a wavelength range from 360-980 nm and have resolution of 2000-5500 depending on the wavelength. The DESI instrument is designed for a 14,000 sq. deg. multi-year survey of targets that trace the evolution of dark energy out to redshift 3.5 using the redshifts of luminous red galaxies (LRGs), emission line galaxies (ELGs) and quasars. DESI is the successor to the successful Stage-III BOSS spectroscopic redshift survey and complements imaging surveys such as the Stage-III Dark Energy Survey (DES, currently operating) and the Stage-IV Large Synoptic Survey Telescope (LSST, planned start early in the next decade).

Smoke Exposure Causes Endoplasmic Reticulum Stress and Lipid Accumulation in Retinal Pigment Epithelium through Oxidative Stress and Complement Activation*

PubMed Central

Kunchithapautham, Kannan; Atkinson, Carl; Rohrer, Bärbel

2014-01-01

Age-related macular degeneration (AMD) is a complex disease caused by genetic and environmental factors, including genetic variants in complement components and smoking. Smoke exposure leads to oxidative stress, complement activation, endoplasmic reticulum (ER) stress, and lipid dysregulation, which have all been proposed to be associated with AMD pathogenesis. Here we examine the effects of smoke exposure on the retinal pigment epithelium (RPE). Mice were exposed to cigarette smoke or filtered air for 6 months. RPE cells grown as stable monolayers were exposed to 5% cigarette smoke extract (CSE). Effects of smoke were determined by biochemical, molecular, and histological measures. Effects of the alternative pathway (AP) of complement and complement C3a anaphylatoxin receptor signaling were analyzed using knock-out mice or specific inhibitors. ER stress markers were elevated after smoke exposure in RPE of intact mice, which was eliminated in AP-deficient mice. To examine this relationship further, RPE monolayers were exposed to CSE. Short term smoke exposure resulted in production and release of complement C3, the generation of C3a, oxidative stress, complement activation on the cell membrane, and ER stress. Long term exposure to CSE resulted in lipid accumulation, and secretion. All measures were reversed by blocking C3a complement receptor (C3aR), alternative complement pathway signaling, and antioxidant therapy. Taken together, our results provide clear evidence that smoke exposure results in oxidative stress and complement activation via the AP, resulting in ER stress-mediated lipid accumulation, and further suggesting that oxidative stress and complement act synergistically in the pathogenesis of AMD. PMID:24711457

Protective immune responses against West Nile virus are primed by distinct complement activation pathways.

PubMed

Mehlhop, Erin; Diamond, Michael S

2006-05-15

West Nile virus (WNV) causes a severe infection of the central nervous system in several vertebrate animals including humans. Prior studies have shown that complement plays a critical role in controlling WNV infection in complement (C) 3(-/-) and complement receptor 1/2(-/-) mice. Here, we dissect the contributions of the individual complement activation pathways to the protection from WNV disease. Genetic deficiencies in C1q, C4, factor B, or factor D all resulted in increased mortality in mice, suggesting that all activation pathways function together to limit WNV spread. In the absence of alternative pathway complement activation, WNV disseminated into the central nervous system at earlier times and was associated with reduced CD8+ T cell responses yet near normal anti-WNV antibody profiles. Animals lacking the classical and lectin pathways had deficits in both B and T cell responses to WNV. Finally, and somewhat surprisingly, C1q was required for productive infection in the spleen but not for development of adaptive immune responses after WNV infection. Our results suggest that individual pathways of complement activation control WNV infection by priming adaptive immune responses through distinct mechanisms.

What is going on up there? - The Chukchi Sea Ecosystem Mooring

NASA Astrophysics Data System (ADS)

Janzen, C.; McCammon, M.; Danielson, S. L.; Winsor, P.; Hopcroft, R. R.; Lalande, C.; Stafford, K.; Hauri, C.; McDonnell, A. M. P.

2016-02-01

As Arctic regions are projected to strongly reflect the impacts of a changing climate, an effort is underway to make sustained, year-round measurements of concurrent physical and biogeochemical parameters in the Arctic. Deploying highly instrumented year-round moorings in the water is no simple feat, given harsh Arctic conditions that include the presence of sea ice and deep ice keels during much of the year. Enter the late-breaking ecosystem mooring located in the northeast Chukchi Sea. This mooring complements established biophysical moorings elsewhere in the northern Bering and Chukchi seas, including those maintained by NOAA-PMEL (M8), UW-APL (Bering Strait) and JAMSTEC moorings. (southern Chukchi and Barrow Canyon). The mooring described here is located on the southern flank of Hanna Shoal and provides a multi-disciplinary approach to year-round observations within a biological hotspot. The Chukchi Ecosystem Mooring is equipped with a sensor suite aimed to monitor and document the state of ocean acidification, nutrient and carbon cycles, particles, waves, currents and physical properties, and even passive and active acoustic monitoring for zooplankton, fish, and marine mammals. Having the simultaneous interdisciplinary measurements provides data valuable to an ecosystem-based approach to research and resource management. The fully outfitted observatory is providing an unprecedented view into the mechanistic workings of the Chukchi Shelf Ecosystem. The first mooring was deployed in September 2014 and recovered in August 2015. The August 2015 deployment consisted of three moorings, each with incremental sensor packages to complete the ecosystem sensor suite. The mooring construction and instrumentation are described in detail, including introduction to the advances in sensor technologies that enable such deployments. Year one data recovery summaries and plots are provided to demonstrate the capabilities.

Update on hemolytic uremic syndrome: Diagnostic and therapeutic recommendations

PubMed Central

Salvadori, Maurizio; Bertoni, Elisabetta

2013-01-01

Hemolytic uremic syndrome (HUS) is a rare disease. In this work the authors review the recent findings on HUS, considering the different etiologic and pathogenetic classifications. New findings in genetics and, in particular, mutations of genes that encode the complement-regulatory proteins have improved our understanding of atypical HUS. Similarly, the complement proteins are clearly involved in all types of thrombotic microangiopathy: typical HUS, atypical HUS and thrombotic thrombocytopenic purpura (TTP). Furthermore, several secondary HUS appear to be related to abnormalities in complement genes in predisposed patients. The authors highlight the therapeutic aspects of this rare disease, examining both “traditional therapy” (including plasma therapy, kidney and kidney-liver transplantation) and “new therapies”. The latter include anti-Shiga-toxin antibodies and anti-C5 monoclonal antibody “eculizumab”. Eculizumab has been recently launched for the treatment of the atypical HUS, but it appears to be effective in the treatment of typical HUS and in TTP. Future therapies are in phases I and II. They include anti-C5 antibodies, which are more purified, less immunogenic and absorbed orally and, anti-C3 antibodies, which are more powerful, but potentially less safe. Additionally, infusions of recombinant complement-regulatory proteins are a potential future therapy. PMID:24255888

Individualization and quality improvement: two new scales to complement measurement of program fidelity.

PubMed

Bond, Gary R; Drake, Robert E; Rapp, Charles A; McHugo, Gregory J; Xie, Haiyi

2009-09-01

Fidelity scales have been widely used to assess program adherence to the principles of an evidence-based practice, but they do not measure important aspects of quality of care. Pragmatic scales measuring clinical quality of services are needed to complement fidelity scales measuring structural aspects of program implementation. As part of the instrumentation developed for the National Implementing Evidence-Based Practices Project, we piloted a new instrument with two 5-item quality scales, Individualization (a client-level quality scale) and Quality Improvement (an organizational-level quality scale). Pairs of independent fidelity assessors conducted fidelity reviews in 49 sites in 8 states at baseline and at four subsequent 6-month intervals over a 2-year follow-up period. The assessors followed a standardized protocol to administer these quality scales during daylong site visits; during these same visits they assessed programs on fidelity to the evidence-based practice that the site was seeking to implement. Assessors achieved acceptable interrater reliability for both Individualization and Quality Improvement. Principal components factor analysis confirmed the 2-scale structure. The two scales were modestly correlated with each other and with the evidence-based practice fidelity scales. Over the first year, Individualization and Quality Improvement improved, but showed little or no improvement during the last year of follow-up. The two newly developed scales showed adequate psychometric properties in this preliminary study, but further research is needed to assess their validity and utility in routine clinical practice.

A novel factor H-Fc chimeric immunotherapeutic molecule against Neisseria gonorrhoeae

PubMed Central

Shaughnessy, Jutamas; Gulati, Sunita; Agarwal, Sarika; Unemo, Magnus; Ohnishi, Makoto; Su, Xia-Hong; Monks, Brian G.; Visintin, Alberto; Madico, Guillermo; Lewis, Lisa A.; Golenbock, Douglas T.; Reed, George W.; Rice, Peter A.; Ram, Sanjay

2015-01-01

Neisseria gonorrhoeae (Ng), the causative agent of the sexually transmitted infection gonorrhea, has developed resistance to almost every conventional antibiotic. There is an urgent need to develop novel therapies against gonorrhea. Many pathogens, including Ng, bind the complement inhibitor factor H (FH) to evade complement-dependent killing. Sialylation of gonococcal lipooligosaccharide, as occurs in vivo, augments binding of human FH through its domains 18-20 (FH18-20). We explored the utility of fusing FH18-20 with IgG Fc (FH18-20/Fc) to create a novel anti-infective immunotherapeutic. FH18-20 also binds to select host glycosaminoglycans to limit unwanted complement activation on host cells. To identify mutation(s) in FH18-20 that eliminated complement activation on host cells, yet maintained binding to Ng, we created four mutations in domains 19 or 20 described in atypical hemolytic uremic syndrome that prevented binding of mutated fH to human erythrocytes. One of the mutant proteins (D to G at position 1119 in domain 19; FHD1119G/Fc) facilitated complement-dependent killing of gonococci similar to unmodified FH18-20/Fc, but unlike FH18-20/Fc, did not lyse human erythrocytes. FHD1119G/Fc bound to all (100%) of 15 sialylated clinical Ng isolates tested (including three contemporary ceftriaxone-resistant strains), mediated complement-dependent killing of 10/15 (67%) strains and enhanced C3 deposition (≥10-fold above baseline levels) on each of the five isolates not directly killed by complement. FHD1119G/Fc facilitated opsonophagocytic killing of a serum-resistant strain by human polymorphonuclear neutrophils. FHD1119G/Fc administered intravaginally significantly reduced the duration and burden of gonococcal infection in the mouse vaginal colonization model. FHD1119G/Fc represents a novel immunotherapeutic against multidrug-resistant Ng. PMID:26773149

Variants in Complement Factor H and Complement Factor H-Related Protein Genes, CFHR3 and CFHR1, Affect Complement Activation in IgA Nephropathy.

PubMed

Zhu, Li; Zhai, Ya-Ling; Wang, Feng-Mei; Hou, Ping; Lv, Ji-Cheng; Xu, Da-Min; Shi, Su-Fang; Liu, Li-Jun; Yu, Feng; Zhao, Ming-Hui; Novak, Jan; Gharavi, Ali G; Zhang, Hong

2015-05-01

Complement activation is common in patients with IgA nephropathy (IgAN) and associated with disease severity. Our recent genome-wide association study of IgAN identified susceptibility loci on 1q32 containing the complement regulatory protein-encoding genes CFH and CFHR1-5, with rs6677604 in CFH as the top single-nucleotide polymorphism and CFHR3-1 deletion (CFHR3-1∆) as the top signal for copy number variation. In this study, to explore the clinical effects of variation in CFH, CFHR3, and CFHR1 on IgAN susceptibility and progression, we enrolled two populations. Group 1 included 1178 subjects with IgAN and available genome-wide association study data. Group 2 included 365 subjects with IgAN and available clinical follow-up data. In group 1, rs6677604 was associated with mesangial C3 deposition by genotype-phenotype correlation analysis. In group 2, we detected a linkage between the rs6677604-A allele and CFHR3-1∆ and found that the rs6677604-A allele was associated with higher serum levels of CFH and lower levels of the complement activation split product C3a. Furthermore, CFH levels were positively associated with circulating C3 levels and negatively associated with mesangial C3 deposition. Moreover, serum levels of the pathogenic galactose-deficient glycoform of IgA1 were also associated with the degree of mesangial C3 deposition in patients with IgAN. Our findings suggest that genetic variants in CFH, CFHR3, and CFHR1 affect complement activation and thereby, predispose patients to develop IgAN. Copyright © 2015 by the American Society of Nephrology.

Strategy for earth explorers in global earth sciences

NASA Technical Reports Server (NTRS)

1988-01-01

The goal of the current NASA Earth System Science initiative is to obtain a comprehensive scientific understanding of the Earth as an integrated, dynamic system. The centerpiece of the Earth System Science initiative will be a set of instruments carried on polar orbiting platforms under the Earth Observing System program. An Earth Explorer program can open new vistas in the earth sciences, encourage innovation, and solve critical scientific problems. Specific missions must be rigorously shaped by the demands and opportunities of high quality science and must complement the Earth Observing System and the Mission to Planet Earth. The committee believes that the proposed Earth Explorer program provides a substantial opportunity for progress in the earth sciences, both through independent missions and through missions designed to complement the large scale platforms and international research programs that represent important national commitments. The strategy presented is intended to help ensure the success of the Earth Explorer program as a vital stimulant to the study of the planet.

Spacelab dedicated discipline laboratory (DDL) utilization concept

NASA Technical Reports Server (NTRS)

Wunsch, P.; De Sanctis, C.

1984-01-01

The dedicated discipline laboratory (DDL) concept is a new approach for implementing Spacelab missions that involves the grouping of science instruments into mission complements of single or compatible disciplines. These complements are evolved in such a way that the DDL payloads can be left intact between flights. This requires the dedication of flight hardware to specific payloads on a long-term basis and raises the concern that the purchase of additional flight hardware will be required to implement the DDL program. However, the payoff is expected to result in significant savings in mission engineering and assembly effort. A study has been conducted recently to quantify both the requirements for new hardware and the projected mission cost savings. It was found that some incremental additions to the current inventory will be needed to fly the mission model assumed. Cost savings of $2M to 6.5M per mission were projected in areas analyzed in depth, and additional savings may occur in areas for which detailed cost data were not available.

Studying the inner regions of young stars and their disks with aperture masking interferometry

NASA Astrophysics Data System (ADS)

Greenbaum, Alexandra; Sivaramakrishnan, Anand; GPI Instrument Team; NIRISS Instrument Team

2017-01-01

High resolution aperture masking interferometry complements coronagraphic imagers to provide a unique perspective on star and planet formation at more moderate contrast. By targeting young stars, especially those with disks, we aim to understand complex protoplanetary environments. Ground-based non-redundant masking (NRM) paired with spectrographs and polarimeters probes both thermally emitting young companions, possibly embedded in the disk or gap and scattered light in protoplanetary disks. And soon the community will have access to the most stable NRM conditions yet, with the Near Infrared Imager and Slitless Spectrograph (NIRISS) Aperture Masking Interferometry (AMI) mode on the James Webb Space Telescope. I will present my thesis work commissioning the Gemini Planet Imager’s NRM, highlighting results through both its spectroscopy and polarimetry modes, which set the stage for future space-based imaging. I will also give an overview of NIRISS-AMI capabilities and performance predictions for imaging young low-mass companions and disks, and how it will complement other instruments on JWST.

Complement in Action: An Analysis of Patent Trends from 1976 Through 2011.

PubMed

Yang, Kun; Deangelis, Robert A; Reed, Janet E; Ricklin, Daniel; Lambris, John D

2013-01-01

Complement is an essential part of the innate immune response. It interacts with diverse endogenous pathways and contributes to the maintenance of homeostasis, the modulation of adaptive immune responses, and the development of various pathologies. The potential usefulness, in both research and clinical settings, of compounds that detect or modulate complement activity has resulted in thousands of publications on complement-related innovations in fields such as drug discovery, disease diagnosis and treatment, and immunoassays, among others. This study highlights the distribution and publication trends of patents related to the complement system that were granted by the United States Patent and Trademark Office from 1976 to the present day. A comparison to complement-related documents published by the World Intellectual Property Organization is also included. Statistical analyses revealed increasing diversity in complement-related research interests over time. More than half of the patents were found to focus on the discovery of inhibitors; interest in various inhibitor classes exhibited a remarkable transformation from chemical compounds early on to proteins and antibodies in more recent years. Among clinical applications, complement proteins and their modulators have been extensively patented for the diagnosis and treatment of eye diseases (especially age-related macular degeneration), graft rejection, cancer, sepsis, and a variety of other inflammatory and immune diseases. All of the patents discussed in this chapter, as well as those from other databases, are available from our newly constructed complement patent database: www.innateimmunity.us/patent .

Complement in action: an analysis of patent trends from 1976 through 2011.

PubMed

Yang, Kun; DeAngelis, Robert A; Reed, Janet E; Ricklin, Daniel; Lambris, John D

2013-01-01

Complement is an essential part of the innate immune response. It interacts with diverse endogenous pathways and contributes to the maintenance of homeostasis, the modulation of adaptive immune responses, and the development of various pathologies. The potential usefulness, in both research and clinical settings, of compounds that detect or modulate complement activity has resulted in thousands of publications on complement-related innovations in fields such as drug discovery, disease diagnosis and treatment, and immunoassays, among others. This study highlights the distribution and publication trends of patents related to the complement system that were granted by the United States Patent and Trademark Office from 1976 to the present day. A comparison to complement-related documents published by the World Intellectual Property Organization is also included. Statistical analyses revealed increasing diversity in complement-related research interests over time. More than half of the patents were found to focus on the discovery of inhibitors; interest in various inhibitor classes exhibited a remarkable transformation from chemical compounds early on to proteins and antibodies in more recent years. Among clinical applications, complement proteins and their modulators have been extensively patented for the diagnosis and treatment of eye diseases (especially age-related macular degeneration), graft rejection, cancer, sepsis, and a variety of other inflammatory and immune diseases. All of the patents discussed in this chapter, as well as those from other databases, are available from our newly constructed complement patent database: www.innateimmunity.us/patent.

Molecular mechanisms of inflammation and tissue injury after major trauma-is complement the "bad guy"?

PubMed Central

2011-01-01

Trauma represents the leading cause of death among young people in industrialized countries. Recent clinical and experimental studies have brought increasing evidence for activation of the innate immune system in contributing to the pathogenesis of trauma-induced sequelae and adverse outcome. As the "first line of defense", the complement system represents a potent effector arm of innate immunity, and has been implicated in mediating the early posttraumatic inflammatory response. Despite its generic beneficial functions, including pathogen elimination and immediate response to danger signals, complement activation may exert detrimental effects after trauma, in terms of mounting an "innocent bystander" attack on host tissue. Posttraumatic ischemia/reperfusion injuries represent the classic entity of complement-mediated tissue damage, adding to the "antigenic load" by exacerbation of local and systemic inflammation and release of toxic mediators. These pathophysiological sequelae have been shown to sustain the systemic inflammatory response syndrome after major trauma, and can ultimately contribute to remote organ injury and death. Numerous experimental models have been designed in recent years with the aim of mimicking the inflammatory reaction after trauma and to allow the testing of new pharmacological approaches, including the emergent concept of site-targeted complement inhibition. The present review provides an overview on the current understanding of the cellular and molecular mechanisms of complement activation after major trauma, with an emphasis of emerging therapeutic concepts which may provide the rationale for a "bench-to-bedside" approach in the design of future pharmacological strategies. PMID:22129197

HIRIS (High-Resolution Imaging Spectrometer: Science opportunities for the 1990s. Earth observing system. Volume 2C: Instrument panel report

NASA Technical Reports Server (NTRS)

1987-01-01

The high-resolution imaging spectrometer (HIRIS) is an Earth Observing System (EOS) sensor developed for high spatial and spectral resolution. It can acquire more information in the 0.4 to 2.5 micrometer spectral region than any other sensor yet envisioned. Its capability for critical sampling at high spatial resolution makes it an ideal complement to the MODIS (moderate-resolution imaging spectrometer) and HMMR (high-resolution multifrequency microwave radiometer), lower resolution sensors designed for repetitive coverage. With HIRIS it is possible to observe transient processes in a multistage remote sensing strategy for Earth observations on a global scale. The objectives, science requirements, and current sensor design of the HIRIS are discussed along with the synergism of the sensor with other EOS instruments and data handling and processing requirements.

A Holographic Microscope for Detection of Microorganisms on Icy Worlds

NASA Astrophysics Data System (ADS)

Lindensmith, C. A.; Nadeau, J. L.; Deming, J. W.; Showalter, G. M.; Rider, S.; Bedrossian, M.

2015-12-01

Holography is a well-established imaging technique that uses the interference of light to record and reproduce three-dimensional images of objects. Its use began in the 1960s with the invention of the laser. Digital holographic microscopy (DHM) has several advantages over ordinary imaging microscopy which make it ideal for field and astrobiology use, including no need for focus or scanning so that instruments are readily made autonomous. DHM can produce simultaneous bright-field and quantitative phase-contrast images of the same field, providing additional information about transparent objects, e.g., refractive index and/or thickness; thus it inherently supports effective label-free imaging. We have built a fieldable DHM for detection of microorganisms in bodies of water and in brines collected from sea ice. Ice that appears solid to the eye contains interconnected brine-filled microscopic pores and veins which are occupied by populations of prokaryotes and eukaryotes. The presence of life in "solid" ice has important implications for exploration of icy worlds, where it is unlikely that the first missions will be able to access the subsurface oceans. Using this new instrument, we examined several dozen samples from three different sites around Nuuk, Greenland. In all samples, mixed populations of both prokaryotic and eukaryotic microorganisms were observed. Many of the organisms were motile immediately upon extraction from sea ice, and others became motile after warming or addition of sugars and/or amino acids. Meaningful motility was readily distinguished from turbulence or fluid flow. The spatial resolution of the instrument was better than 1 μm, leading to unambiguous recognition of subcellular structures in eukaryotes, including nuclei and chloroplasts. We present mission scenrios for both orbiters and landers in which DHM may be used as a valuable complement to chemical-based life detection techniques for discovery of cellular life on icy worlds.

A Graphical Teaching Tool for Understanding Two's Complement.

ERIC Educational Resources Information Center

Luck, Carlos L.

As part of the Electrical Engineering program at the Univesity of Southern Maine, students are typically introduced to Two's Complement algebra and representation, a method to include negative numbers in the binary representation of integers that is widely used in microprocessors and related digital systems. The traditional, procedural method to…

21 CFR 866.4100 - Complement reagent.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 21 Food and Drugs 8 2012-04-01 2012-04-01 false Complement reagent. 866.4100 Section 866.4100 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL... naturally occurring serum protein from any warm-blooded animal such as guinea pigs, that may be included as...

21 CFR 866.4100 - Complement reagent.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 21 Food and Drugs 8 2014-04-01 2014-04-01 false Complement reagent. 866.4100 Section 866.4100 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL... naturally occurring serum protein from any warm-blooded animal such as guinea pigs, that may be included as...

21 CFR 866.4100 - Complement reagent.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 21 Food and Drugs 8 2013-04-01 2013-04-01 false Complement reagent. 866.4100 Section 866.4100 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL... naturally occurring serum protein from any warm-blooded animal such as guinea pigs, that may be included as...

21 CFR 866.4100 - Complement reagent.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 21 Food and Drugs 8 2011-04-01 2011-04-01 false Complement reagent. 866.4100 Section 866.4100 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL... naturally occurring serum protein from any warm-blooded animal such as guinea pigs, that may be included as...

Innate Immune Mechanisms in Transplant Allograft Vasculopathy

PubMed Central

Jane-wit, D; Fang, C; Goldstein, DR

2016-01-01

Purpose of Review Allograft vasculopathy (AV) is the leading cause of late allograft loss following solid organ transplantation. Ischemia reperfusion injury (IRI) and donor specific antibody (DSA)-induced complement activation confer heightened risk for AV via numerous innate immune mechanisms including MyD88, HMGB1, and complement induced non-canonical NF-kB signaling. Recent Findings The role of MyD88, a signal adaptor downstream of the toll-like receptors (TLR), has been defined in an experimental heart transplant model, which demonstrated that recipient MyD88 enhanced AV. Importantly, triggering receptor on myeloid receptor 1(Trem1), a MyD88 amplifying signal, was present in rejecting human cardiac transplant biopsies and enhanced the development of AV in mice. HMGB1, a nuclear protein that activates TLRs, also enhanced the development of AV. Complement activation elicits assembly of membrane attack complexes (MAC) on endothelial cells which activate non-canonical NF-kB signaling, a novel complement effector pathway that induces pro-inflammatory genes and potentiates endothelial cell mediated alloimmune T cell activation, processes which enhance AV. Summary Innate immune mediators including HMGB1, MyD88, and non-canonical NFκB signaling via complement activation contribute to AV. These pathways represent potential therapeutic targets to reduce AV after solid organ transplantation. PMID:27077602

Antimicrobial Peptides and Complement in Neonatal Hypoxia-Ischemia Induced Brain Damage

PubMed Central

Rocha-Ferreira, Eridan; Hristova, Mariya

2015-01-01

Hypoxic-ischemic encephalopathy (HIE) is a clinical condition in the neonate, resulting from oxygen deprivation around the time of birth. HIE affects 1–5/1000 live births worldwide and is associated with the development of neurological deficits, including cerebral palsy, epilepsy, and cognitive disabilities. Even though the brain is considered as an immune-privileged site, it has innate and adaptive immune response and can produce complement (C) components and antimicrobial peptides (AMPs). Dysregulation of cerebral expression of AMPs and C can exacerbate or ameliorate the inflammatory response within the brain. Brain ischemia triggers a prolonged inflammatory response affecting the progression of injury and secondary energy failure and involves both innate and adaptive immune systems, including immune-competent and non-competent cells. Following injury to the central nervous system (CNS), including neonatal hypoxia-ischemia (HI), resident microglia, and astroglia are the main cells providing immune defense to the brain in a stimulus-dependent manner. They can express and secrete pro-inflammatory cytokines and therefore trigger prolonged inflammation, resulting in neurodegeneration. Microglial cells express and release a wide range of inflammation-associated molecules including several components of the complement system. Complement activation following neonatal HI injury has been reported to contribute to neurodegeneration. Astrocytes can significantly affect the immune response of the CNS under pathological conditions through production and release of pro-inflammatory cytokines and immunomodulatory AMPs. Astrocytes express β-defensins, which can chemoattract and promote maturation of dendritic cells (DC), and can also limit inflammation by controlling the viability of these same DC. This review will focus on the balance of complement components and AMPs within the CNS following neonatal HI injury and the effect of that balance on the subsequent brain damage. PMID:25729383

Cyclosporine Induces Endothelial Cell Release of Complement-Activating Microparticles

PubMed Central

Renner, Brandon; Klawitter, Jelena; Goldberg, Ryan; McCullough, James W.; Ferreira, Viviana P.; Cooper, James E.; Christians, Uwe

2013-01-01

Defective control of the alternative pathway of complement is an important risk factor for several renal diseases, including atypical hemolytic uremic syndrome. Infections, drugs, pregnancy, and hemodynamic insults can trigger episodes of atypical hemolytic uremic syndrome in susceptible patients. Although the mechanisms linking these clinical events with disease flares are unknown, recent work has revealed that each of these clinical conditions causes cells to release microparticles. We hypothesized that microparticles released from injured endothelial cells promote intrarenal complement activation. Calcineurin inhibitors cause vascular and renal injury and can trigger hemolytic uremic syndrome. Here, we show that endothelial cells exposed to cyclosporine in vitro and in vivo release microparticles that activate the alternative pathway of complement. Cyclosporine-induced microparticles caused injury to bystander endothelial cells and are associated with complement-mediated injury of the kidneys and vasculature in cyclosporine-treated mice. Cyclosporine-induced microparticles did not bind factor H, an alternative pathway regulatory protein present in plasma, explaining their complement-activating phenotype. Finally, we found that in renal transplant patients, the number of endothelial microparticles in plasma increases 2 weeks after starting tacrolimus, and treatment with tacrolimus associated with increased C3 deposition on endothelial microparticles in the plasma of some patients. These results suggest that injury-associated release of endothelial microparticles is an important mechanism by which systemic insults trigger intravascular complement activation and complement-dependent renal diseases. PMID:24092930

Complement activation by carbon nanotubes and its influence on the phagocytosis and cytokine response by macrophages.

PubMed

Pondman, Kirsten M; Sobik, Martin; Nayak, Annapurna; Tsolaki, Anthony G; Jäkel, Anne; Flahaut, Emmanuel; Hampel, Silke; Ten Haken, Bennie; Sim, Robert B; Kishore, Uday

2014-08-01

Carbon nanotubes (CNTs) have promised a range of applications in biomedicine. Although influenced by the dispersants used, CNTs are recognized by the innate immune system, predominantly by the classical pathway of the complement system. Here, we confirm that complement activation by the CNT used continues up to C3 and C5, indicating that the entire complement system is activated including the formation of membrane-attack complexes. Using recombinant forms of the globular regions of human C1q (gC1q) as inhibitors of CNT-mediated classical pathway activation, we show that C1q, the first recognition subcomponent of the classical pathway, binds CNTs via the gC1q domain. Complement opsonisation of CNTs significantly enhances their uptake by U937 cells, with concomitant downregulation of pro-inflammatory cytokines and up-regulation of anti-inflammatory cytokines in both U937 cells and human monocytes. We propose that CNT-mediated complement activation may cause recruitment of cellular infiltration, followed by phagocytosis without inducing a pro-inflammatory immune response. This study highlights the importance of the complement system in response to carbon nanontube administration, suggesting that the ensuing complement activation may cause recruitment of cellular infiltration, followed by phagocytosis without inducing a pro-inflammatory immune response. Copyright © 2014 Elsevier Inc. All rights reserved.

Complement factor H in host defense and immune evasion.

PubMed

Parente, Raffaella; Clark, Simon J; Inforzato, Antonio; Day, Anthony J

2017-05-01

Complement is the major humoral component of the innate immune system. It recognizes pathogen- and damage-associated molecular patterns, and initiates the immune response in coordination with innate and adaptive immunity. When activated, the complement system unleashes powerful cytotoxic and inflammatory mechanisms, and thus its tight control is crucial to prevent damage to host tissues and allow restoration of immune homeostasis. Factor H is the major soluble inhibitor of complement, where its binding to self markers (i.e., particular glycan structures) prevents complement activation and amplification on host surfaces. Not surprisingly, mutations and polymorphisms that affect recognition of self by factor H are associated with diseases of complement dysregulation, such as age-related macular degeneration and atypical haemolytic uremic syndrome. In addition, pathogens (i.e., non-self) and cancer cells (i.e., altered-self) can hijack factor H to evade the immune response. Here we review recent (and not so recent) literature on the structure and function of factor H, including the emerging roles of this protein in the pathophysiology of infectious diseases and cancer.

TerraHertz Free Electron Laser Applications for Satellite Remote Sensing

NASA Technical Reports Server (NTRS)

Heaps, William S.

2003-01-01

The development of a Free Electron Laser (EL) operating in the terahertz frequency regime by the group at the University of Hawaii (Elias et al.) represents a significant new opportunity in the area of atmospheric remote sensing. The FEL has 2 salient features that create a unique opportunity. First of all it represents the only source in this frequency range with sufficient power to enable lidar instrumentation. Secondly its very high electrical efficiency (several times more efficient than any currently employed spaceborne laser) renders it a strong candidate for use in satellite remote sensing. On the negative side the atmosphere is rather strongly absorbing throughout this frequency range due primarily to the water vapor continuum absorption. This means that the instruments using this laser will not be able to access the lower troposphere because of its very high water concentration.. However the instrument will be very capable of measurements in the upper troposphere and stratosphere. A passive instrument, the Microwave Limb Sounder on the UARS satellite operated by Jet Propulsion Laboratory, has already demonstrated that this wavelength region can be used for chemical species with strong emission lines. A lidar would complement the capabilities of this instrument by providing the capability to measure absorbing species in the upper atmosphere. I will discuss the design of such an instrument in greater detail and estimate its performance in measuring a number of chemical species of interest to the Earth Science community.

The Lunar Crater Observation and Sensing Satellite (LCROSS) Payload Development and Performance in Flight

NASA Astrophysics Data System (ADS)

Ennico, Kimberly; Shirley, Mark; Colaprete, Anthony; Osetinsky, Leonid

2012-05-01

The primary objective of the Lunar Crater Observation and Sensing Satellite (LCROSS) was to confirm the presence or absence of water ice in a permanently shadowed region (PSR) at a lunar pole. LCROSS was classified as a NASA Class D mission. Its payload, the subject of this article, was designed, built, tested and operated to support a condensed schedule, risk tolerant mission approach, a new paradigm for NASA science missions. All nine science instruments, most of them ruggedized commercial-off-the-shelf (COTS), successfully collected data during all in-flight calibration campaigns, and most importantly, during the final descent to the lunar surface on October 9, 2009, after 112 days in space. LCROSS demonstrated that COTS instruments and designs with simple interfaces, can provide high-quality science at low-cost and in short development time frames. Building upfront into the payload design, flexibility, redundancy where possible even with the science measurement approach, and large margins, played important roles for this new type of payload. The environmental and calibration approach adopted by the LCROSS team, compared to existing standard programs, is discussed. The description, capabilities, calibration and in-flight performance of each instrument are summarized. Finally, this paper goes into depth about specific areas where the instruments worked differently than expected and how the flexibility of the payload team, the knowledge of instrument priority and science trades, and proactive margin maintenance, led to a successful science measurement by the LCROSS payload's instrument complement.

Anti-complementary constituents of Houttuynia cordata and their targets in complement activation cascade.

PubMed

Jiang, Yun; Lu, Yan; Zhang, Yun-Yi; Chen, Dao-Feng

2014-01-01

Activity-guided fractionation for complement inhibitors led to the isolation of 23 known compounds from Houttuynia cordata Thunb. Seven flavonoids, two alkaloids, one coumarin and two phenols showed anti-complementary activity. Preliminary inhibitory mechanism of four flavonoids, including quercitrin, afzelin, isoquercitrin and quercetin in the complement activation cascade were examined for the first time. The results indicated that the target components of flavonols are different from those of flavonosides, and the glycoside moieties may be necessary to block C3 and C4 components.

Jupiter Trojan's Shallow Subsurface: Direct Observation By Radar Sounding

NASA Astrophysics Data System (ADS)

Herique, A.; Plettemeier, D.; Beck, P.; Michel, P.; Kumamoto, A.; Kofman, W. W.

2017-12-01

Most of the Jupiter's Trojan are classified as spectral type P or D from visible and near-IR observations. Still, major question remain regarding theire origin and geological evolution: What ices are present in their interior, and in what amount? What is the abundance and the nature of the organic fraction? Did they experience some level of differentiation powered by 26Al? Answering theses question is the goal of the Solar-Power Sail JAXA mission [1, 2]. This mission plans to study the surface by remote sensing in the optical in IR domain. This probe will carry a large-sized lander with a drill to sample the constitutive material at meter depth in order to complement physical and chemical properties measured by on-board instruments. The sample return is an option under study.Radar sounding of the shallow subsurface would be envisaged in complement to this payload. Sounding radar could provide the structure of the first tens of meters of the Trojan surface. It will allow identifying layering, ice lens, and embedded block. It also will enable to reconnect the surface with the deep interior in order to identify exogenous / pristine material. For the surface package, the drilling and the sample return, radar sounding is a unique opportunity to support the selection of the landing site and to provide the greater geological context of the samples that will be returned to Earth.In this paper, we will detail the objective of this instrument and then we will outline the proposed instrument, which is inheriting from the radar developed for the AIDA/AIM mission.[1] Mori, O. et al., Science experiments on a Jupiter Trojan Asteroid in the solar powerd sail mission. LPSC 2016 - 1822.[2] Okada, T. et al., Science and Exploration of a Jupiter Trojan Asteroid in the solar-power sail mission. LPSC 2017 - 1828.

Identification of C3b-Binding Small-Molecule Complement Inhibitors Using Cheminformatics.

PubMed

Garcia, Brandon L; Skaff, D Andrew; Chatterjee, Arindam; Hanning, Anders; Walker, John K; Wyckoff, Gerald J; Geisbrecht, Brian V

2017-05-01

The complement system is an elegantly regulated biochemical cascade formed by the collective molecular recognition properties and proteolytic activities of more than two dozen membrane-bound or serum proteins. Complement plays diverse roles in human physiology, such as acting as a sentry against invading microorganisms, priming of the adaptive immune response, and removal of immune complexes. However, dysregulation of complement can serve as a trigger for a wide range of human diseases, which include autoimmune, inflammatory, and degenerative conditions. Despite several potential advantages of modulating complement with small-molecule inhibitors, small-molecule drugs are highly underrepresented in the current complement-directed therapeutics pipeline. In this study, we have employed a cheminformatics drug discovery approach based on the extensive structural and functional knowledge available for the central proteolytic fragment of the cascade, C3b. Using parallel in silico screening methodologies, we identified 45 small molecules that putatively bind C3b near ligand-guided functional hot spots. Surface plasmon resonance experiments resulted in the validation of seven dose-dependent C3b-binding compounds. Competition-based biochemical assays demonstrated the ability of several C3b-binding compounds to interfere with binding of the original C3b ligand that guided their discovery. In vitro assays of complement function identified a single complement inhibitory compound, termed cmp-5, and mechanistic studies of the cmp-5 inhibitory mode revealed it acts at the level of C5 activation. This study has led to the identification of a promising new class of C3b-binding small-molecule complement inhibitors and, to our knowledge, provides the first demonstration of cheminformatics-based, complement-directed drug discovery. Copyright © 2017 by The American Association of Immunologists, Inc.

Complement Evasion by Pathogenic Leptospira.

PubMed

Fraga, Tatiana Rodrigues; Isaac, Lourdes; Barbosa, Angela Silva

2016-01-01

Leptospirosis is a neglected infectious disease caused by spirochetes from the genus Leptospira . Pathogenic microorganisms, notably those which reach the blood circulation such as Leptospira , have evolved multiple strategies to escape the host complement system, which is important for innate and acquired immunity. Leptospira avoid complement-mediated killing through: (i) recruitment of host complement regulators; (ii) acquisition of host proteases that cleave complement proteins on the bacterial surface; and, (iii) secretion of proteases that inactivate complement proteins in the Leptospira surroundings. The recruitment of host soluble complement regulatory proteins includes the acquisition of Factor H (FH) and FH-like-1 (alternative pathway), C4b-binding protein (C4BP) (classical and lectin pathways), and vitronectin (Vn) (terminal pathway). Once bound to the leptospiral surface, FH and C4BP retain cofactor activity of Factor I in the cleavage of C3b and C4b, respectively. Vn acquisition by leptospires may result in terminal pathway inhibition by blocking C9 polymerization. The second evasion mechanism lies in plasminogen (PLG) binding to the leptospiral surface. In the presence of host activators, PLG is converted to enzymatically active plasmin, which is able to degrade C3b, C4b, and C5 at the surface of the pathogen. A third strategy used by leptospires to escape from complement system is the active secretion of proteases. Pathogenic, but not saprophytic leptospires, are able to secrete metalloproteases that cleave C3 (central complement molecule), Factor B (alternative pathway), and C4 and C2 (classical and lectin pathways). The purpose of this review is to fully explore these complement evasion mechanisms, which act together to favor Leptospira survival and multiplication in the host.

Complement Evasion by Pathogenic Leptospira

PubMed Central

Fraga, Tatiana Rodrigues; Isaac, Lourdes; Barbosa, Angela Silva

2016-01-01

Leptospirosis is a neglected infectious disease caused by spirochetes from the genus Leptospira. Pathogenic microorganisms, notably those which reach the blood circulation such as Leptospira, have evolved multiple strategies to escape the host complement system, which is important for innate and acquired immunity. Leptospira avoid complement-mediated killing through: (i) recruitment of host complement regulators; (ii) acquisition of host proteases that cleave complement proteins on the bacterial surface; and, (iii) secretion of proteases that inactivate complement proteins in the Leptospira surroundings. The recruitment of host soluble complement regulatory proteins includes the acquisition of Factor H (FH) and FH-like-1 (alternative pathway), C4b-binding protein (C4BP) (classical and lectin pathways), and vitronectin (Vn) (terminal pathway). Once bound to the leptospiral surface, FH and C4BP retain cofactor activity of Factor I in the cleavage of C3b and C4b, respectively. Vn acquisition by leptospires may result in terminal pathway inhibition by blocking C9 polymerization. The second evasion mechanism lies in plasminogen (PLG) binding to the leptospiral surface. In the presence of host activators, PLG is converted to enzymatically active plasmin, which is able to degrade C3b, C4b, and C5 at the surface of the pathogen. A third strategy used by leptospires to escape from complement system is the active secretion of proteases. Pathogenic, but not saprophytic leptospires, are able to secrete metalloproteases that cleave C3 (central complement molecule), Factor B (alternative pathway), and C4 and C2 (classical and lectin pathways). The purpose of this review is to fully explore these complement evasion mechanisms, which act together to favor Leptospira survival and multiplication in the host. PMID:28066433

Temperature-sensitive Mutants of Sindbis Virus: Biochemical Correlates of Complementation

PubMed Central

Burge, Boyce W.; Pfefferkorn, E. R.

1967-01-01

Temperature-sensitive mutants of Sindbis virus fail to grow at a temperature that permits growth of the wild type, but when certain pairs of these mutants, mixed together, infect cells at that temperature, viral growth (i.e., complementation) occurs. The yield from this complementation, however, is of the same order of magnitude as the infectivity in the inoculum. Since in animal virus infections the protein components of the virion probably enter the cell with the viral nucleic acid, it was necessary to demonstrate that the observed complementation required synthesis of new viral protein and nucleic acid rather than some sort of rearrangement of the structural components of the inoculum. To demonstrate that complementation does require new biosynthesis, three biochemical events of normal virus growth have been observed during complementation and correlated with the efficiency of viral growth seen in complementation. These events include: (i) entrance of parental viral ribonucleic acid (RNA) into a double-stranded form; (ii) subsequent synthesis of viral RNA; and (iii) synthesis and subsequent incorporation of viral protein(s) into cell membranes where they were detected by hemadsorption. Although the infecting single-stranded RNA genome of the wild type was converted to a ribonuclease-resistant form, the genome of a mutant (ts-11) incapable of RNA synthesis at a nonpermissive temperature was not so converted. However, during complementation with another mutant also defective in viral RNA synthesis, some of the RNA of mutant ts-11 was converted to a ribonuclease-resistant form, and total synthesis of virus-specific RNA was markedly enhanced. The virus-specific alteration of the cell surface, detected by hemadsorption, was also extensively increased during complementation. These observations support the view that complementation between temperature-sensitive mutants and replication of wild-type virus are similar processes. PMID:5630228

A cosmic and solar X-ray and gamma-ray instrument for a scout launch

NASA Technical Reports Server (NTRS)

Forrest, D. J.; Vestrand, W. T.; Chupp, E. L.

1988-01-01

An overview is presented for a set of simple and robust X-ray and gamma ray instruments which have both cosmic and solar objectives. The primary solar scientific objective is the study of the beaming of energetic electrons and ions in solar flares. The instrument will measure spectra and polarization of flare emissions up to 10 MeV. At X-ray energies both the directly emitted flux and the reflected albedo flux will be measured with a complement of six X-ray sensors. Each of these detectors will have a different high Z filter selected to optimize both the energy resolution and high rate capabilities in the energy band 10 to 300 keV. At energies greater than 100 keV seven 7.6 x 7.6 cm NaI and a set of 30 concentric plastic scattering detectors will record the spectra and polarization of electron bremsstrahlung and nuclear gamma rays. All of the components of the instrument are in existence and have passed flight tests for earlier space missions. The instrument will use a spinning solar oriented Scout spacecraft. The NaI detectors will act as a self-modulating gamma ray detector for cosmic sources in a broad angular band which lies at 90 degrees to the Sun-Earth vector and hence will scan the entire sky in 6 months.

Instruments for Reading Direct-Marked Data-Matrix Symbols

NASA Technical Reports Server (NTRS)

Schramm, Harry F.; Corder, Eric L.

2006-01-01

Improved optoelectronic instruments (specially configured digital cameras) for reading direct-marked data-matrix symbols on the surfaces of optically reflective objects (including specularly reflective ones) are undergoing development. Data-matrix symbols are two-dimensional binary patterns that are used, like common bar codes, for automated identification of objects. The first data-matrix symbols were checkerboard-like patterns of black-and-white rectangles, typically existing in the forms of paint, ink, or detachable labels. The major advantage of direct marking (the marks are more durable than are painted or printed symbols or detachable labels) is offset by a major disadvantage (the marks generated by some marking methods do not provide sufficient contrast to be readable by optoelectronic instruments designed to read black-and-white data-matrix symbols). Heretofore, elaborate lighting, lensing, and software schemes have been tried in efforts to solve the contrast problem in direct-mark matrix- symbol readers. In comparison with prior readers based on those schemes, the readers now undergoing development are expected to be more effective while costing less. All of the prior direct-mark matrix-symbol readers are designed to be aimed perpendicularly to marked target surfaces, and they tolerate very little angular offset. However, the reader now undergoing development not only tolerates angular offset but depends on angular offset as a means of obtaining the needed contrast, as described below. The prototype reader (see Figure 1) includes an electronic camera in the form of a charge-coupled-device (CCD) image detector equipped with a telecentric lens. It also includes a source of collimated visible light and a source of collimated infrared light for illuminating a target. The visible and infrared illumination complement each other: the visible illumination is more useful for aiming the reader toward a target, while the infrared illumination is more useful for reading symbols on highly reflective surfaces. By use of beam splitters, the visible and infrared collimated lights are introduced along the optical path of the telecentric lens, so that the target is illuminated and viewed from the same direction.

Instrumentation for localized superconducting cavity diagnostics

DOE Office of Scientific and Technical Information (OSTI.GOV)

Conway, Z. A.; Ge, M.; Iwashita, Y.

2017-01-12

Superconducting accelerator cavities are now routinely operated at levels approaching the theoretical limit of niobium. To achieve these operating levels more information than is available from the RF excitation signal is required to characterize and determine fixes for the sources of performance limitations. This information is obtained using diagnostic techniques which complement the analysis of the RF signal. In this paper we describe the operation and select results from three of these diagnostic techniques: the use of large scale thermometer arrays, second sound wave defect location and high precision cavity imaging with the Kyoto camera.

Use of Behaviorally Anchored Rating Scales (BARS) to Complement the Management by Objectives (MBO) and Fitness Report Components of the Marine Corps Performance Evaluation System.

DTIC Science & Technology

1980-06-06

performance coaching/ counseling instrument. This conclusion is congruent with current theory on performance appraisal.1 Fred Luthans, in presenting Porter...first characteristic and all of the second and fourth charac- teristics as well. 2 Fred Luthans, Organizational Behavior (2d ed.; New York: McGraw...3d ed. Englewood Clfs, N.J.: Prenfi-ce-Ha- TI -7. Kerlinger, Fred N. Foundations of Behavioral Research. 2d ed. New York: Holt, Rine-art and Winston

Field observations using an AOTF polarimetric imaging spectrometer

NASA Technical Reports Server (NTRS)

Cheng, Li-Jen; Hamilton, Mike; Mahoney, Colin; Reyes, George

1993-01-01

This paper reports preliminary results of recent field observations using a prototype acousto-optic tunable filter (AOTF) polarimetric imaging spectrometer. The data illustrate application potentials for geoscience. The operation principle of this instrument is different from that of current airborne multispectral imaging instruments, such as AVIRIS. The AOTF instrument takes two orthogonally polarized images at a desired wavelength at one time, whereas AVIRIS takes a spectrum over a predetermined wavelength range at one pixel at a time and the image is constructed later. AVIRIS does not have any polarization measuring capability. The AOTF instrument could be a complement tool to AVIRIS. Polarization measurement is a desired capability for many applications in remote sensing. It is well know that natural light is often polarized due to various scattering phenomena in the atmosphere. Also, scattered light from canopies is reported to have a polarized component. To characterize objects of interest correctly requires a remote sensing imaging spectrometer capable of measuring object signal and background radiation in both intensity and polarization so that the characteristics of the object can be determined. The AORF instrument has the capability to do so. The AOTF instrument has other unique properties. For example, it can provide spectral images immediately after the observation. The instrument can also allow observations to be tailored in real time to perform the desired experiments and to collect only required data. Consequently, the performance in each mission can be increased with minimal resources. The prototype instrument was completed in the beginning of this year. A number of outdoor field experiments were performed with the objective to evaluate the capability of this new technology for remote sensing applications and to determine issues for further improvements.

The geostationary Earth radiation budget (GERB) instrument on EUMETSAT's MSG satellite

NASA Astrophysics Data System (ADS)

Sandford, M. C. W.; Allan, P. M.; Caldwell, M. E.; Delderfield, J.; Oliver, M. B.; Sawyer, E.; Harries, J. E.; Ashmall, J.; Brindley, H.; Kellock, S.; Mossavati, R.; Wrigley, R.; Llewellyn-Jones, D.; Blake, O.; Butcher, G.; Cole, R.; Nelms, N.; DeWitte, S.; Gloesener, P.; Fabbrizzi, F.

2003-12-01

Geostationary Earth radiation budget (GERB) is an Announcement of Opportunity Instrument for EUMETSAT's Meteosat Second Generation (MSG) satellite. GERB will make accurate measurements of the Earth Radiation Budget from geostationary orbit, provide an absolute reference calibration for LEO Earth radiation budget instruments and allow studies of the energetics of atmospheric processes. By operating from geostationary orbit, measurements may be made many times a day, thereby providing essentially perfect diurnal sampling of the radiation balance between reflected and emitted radiance for that area of the globe within the field of view. GERB will thus complement other instruments which operate in low orbit and give complete global coverage, but with poor and biased time resolution. GERB measures infrared radiation in two wavelength bands: 0.32-4.0 and 0.32- 30 μm, with a pixel element size of 44 km at sub-satellite point. This paper gives an overview of the project and concentrates on the design and development of the instrument and ground testing and calibration, and lessons learnt from a short time scale low-budget project. The instrument was delivered for integration on the MSG platform in April 1999 ready for the proposed launch in October 2000, which has now been delayed probably to early 2002. The ground segment is being undertaken by RAL and RMIB and produces near real-time data for meteorological applications in conjunction with the main MSG imager—SEVERI. Climate research and other applications which are being developed under a EU Framework IV pilot project will be served by fully processed data. Because of the relevance of the observations to climate change, it is planned to maintain an operating instrument in orbit for at least 3.5 years. Two further GERB instruments are being built for subsequent launches of MSG.

Complement Activation: An Emerging Player in the Pathogenesis of Cardiovascular Disease

PubMed Central

Carter, Angela M.

2012-01-01

A wealth of evidence indicates a fundamental role for inflammation in the pathogenesis of cardiovascular disease (CVD), contributing to the development and progression of atherosclerotic lesion formation, plaque rupture, and thrombosis. An increasing body of evidence supports a functional role for complement activation in the pathogenesis of CVD through pleiotropic effects on endothelial and haematopoietic cell function and haemostasis. Prospective and case control studies have reported strong relationships between several complement components and cardiovascular outcomes, and in vitro studies and animal models support a functional effect. Complement activation, in particular, generation of C5a and C5b-9, influences many processes involved in the development and progression of atherosclerosis, including promotion of endothelial cell activation, leukocyte infiltration into the extracellular matrix, stimulation of cytokine release from vascular smooth muscle cells, and promotion of plaque rupture. Complement activation also influences thrombosis, involving components of the mannose-binding lectin pathway, and C5b-9 in particular, through activation of platelets, promotion of fibrin formation, and impairment of fibrinolysis. The participation of the complement system in inflammation and thrombosis is consistent with the physiological role of the complement system as a rapid effector system conferring protection following vessel injury. However, in the context of CVD, these same processes contribute to development of atherosclerosis, plaque rupture, and thrombosis. PMID:24278688

The quantitative measurement of consciousness during epileptic seizures.

PubMed

Nani, Andrea; Cavanna, Andrea E

2014-01-01

The assessment of consciousness is a fundamental element in the classification of epileptic seizures. It is, therefore, of great importance for clinical practice to develop instruments that enable an accurate and reliable measurement of the alteration of consciousness during seizures. Over the last few years, three psychometric scales have been specifically proposed to measure ictal consciousness: the Ictal Consciousness Inventory (ICI), the Consciousness Seizure Scale (CSS), and the Responsiveness in Epilepsy Scale--versions I and II (RES-I and RES-II). The ICI is a self-report psychometric instrument which retrospectively assesses ictal consciousness along the dimensions of the level/arousal and contents/awareness. The CSS has been used by clinicians to quantify the impairment of consciousness in order to establish correlations with the brain mechanisms underlying alterations of consciousness during temporal lobe seizures. The most recently developed observer-rated instrument is the RES-I, which has been used to assess responsiveness during epileptic seizures in patients undergoing video-EEG. The implementation of standardized psychometric tools for the assessment of ictal consciousness can complement clinical observations and contribute to improve accuracy in seizure classification. © 2013.

Reflectron Time-of-Flight Mass Spectrometer (REMAS) Instrumentation

NASA Technical Reports Server (NTRS)

Brinckerhoff, W. B.; McEntire, R. W.; Cheng, A. F.

2000-01-01

The restricted mass and power budgets of landed science missions present a challenge to obtaining detailed analyses of planetary bodies. In situ studies, whether alone or as reconnaissance for sample return, must rely on highly miniaturized and autonomous instrumentation. Such devices must still produce useful data sets from a minimum of measurements. The great desire to understand the surfaces and interiors of planets, moons, and small bodies had driven the development of small, robotic techniques with ever-increasing capabilities. One of the most important goals on a surface mission is to study composition in many geological contexts. The mineralogical, molecular, elemental, and isotopic content of near-surface materials (regolith, rocks, soils, dust, etc.) at a variety of sites can complement broader imaging to describe the makeup and formative history of the body in question. Instruments that perform this site-to-site analysis must be highly transportable and work as a suite. For instance, a camera, microscope, spectrophotometer, and mass spectrometer can share several components and operate under a parallel command structure. Efficient use of multiple systems on a small rover has been demonstrated on the Mars Pathfinder mission.

Financing long-term care: ex ante, ex post or both?

PubMed

Costa-Font, Joan; Courbage, Christophe; Swartz, Katherine

2015-03-01

This paper attempts to examine the heterogeneity in the public financing of long-term care (LTC) and the wide-ranging instruments in place to finance LTC services. We distinguish and classify the institutional responses to the need for LTC financing as ex ante (occurring prior to when the need arises, such as insurance) and ex post (occurring after the need arises, such as public sector and family financing). Then, we examine country-specific data to ascertain whether the two types of financing are complements or substitutes. Finally, we examine exploratory cross-national data on public expenditure determinants, specifically economic, demographic and social determinants. We show that although both ex ante and ex post mechanisms exist in all countries with advanced industrial economies and despite the fact that instruments are different across countries, ex ante and ex post instruments are largely substitutes for each other. Expenditure estimates to date indicate that the public financing of LTC is highly sensitive to a country's income, ageing of the population and the availability of informal caregiving. Copyright © 2015 John Wiley & Sons, Ltd.

Neurosurgical confocal endomicroscopy: A review of contrast agents, confocal systems, and future imaging modalities

PubMed Central

Zehri, Aqib H.; Ramey, Wyatt; Georges, Joseph F.; Mooney, Michael A.; Martirosyan, Nikolay L.; Preul, Mark C.; Nakaji, Peter

2014-01-01

Background: The clinical application of fluorescent contrast agents (fluorescein, indocyanine green, and aminolevulinic acid) with intraoperative microscopy has led to advances in intraoperative brain tumor imaging. Their properties, mechanism of action, history of use, and safety are analyzed in this report along with a review of current laser scanning confocal endomicroscopy systems. Additional imaging modalities with potential neurosurgical utility are also analyzed. Methods: A comprehensive literature search was performed utilizing PubMed and key words: In vivo confocal microscopy, confocal endomicroscopy, fluorescence imaging, in vivo diagnostics/neoplasm, in vivo molecular imaging, and optical imaging. Articles were reviewed that discussed clinically available fluorophores in neurosurgery, confocal endomicroscopy instrumentation, confocal microscopy systems, and intraoperative cancer diagnostics. Results: Current clinically available fluorescent contrast agents have specific properties that provide microscopic delineation of tumors when imaged with laser scanning confocal endomicroscopes. Other imaging modalities such as coherent anti-Stokes Raman scattering (CARS) microscopy, confocal reflectance microscopy, fluorescent lifetime imaging (FLIM), two-photon microscopy, and second harmonic generation may also have potential in neurosurgical applications. Conclusion: In addition to guiding tumor resection, intraoperative fluorescence and microscopy have the potential to facilitate tumor identification and complement frozen section analysis during surgery by providing real-time histological assessment. Further research, including clinical trials, is necessary to test the efficacy of fluorescent contrast agents and optical imaging instrumentation in order to establish their role in neurosurgery. PMID:24872922

A Detection of the Same Hot Plasma in the Corona: During a CME and Later at Ulysses

NASA Technical Reports Server (NTRS)

Suess, S. T.; Poletto, G.

2004-01-01

We show direct evidence for the same very hot plasma being detected remotely from SOHO in the corona and subsequently, at Ulysses in the solar wind. This is, to our knowledge, the first time that such an unambiguous identification has been made in the case of hot plasma. This detection complements studies correlating other plasma and field properties observed to the properties measured at the source in the corona. This observation takes advantage of a SOHO-Sun-Ulysses quadrature, during which the Sun-Ulysses included angle is $90^\\circ$ and it is possible to observe with Ulysses instruments the same plasma that has previously been remotely observed with SOHO instruments in the corona on the limb of the Sun. The identification builds on an existing base of separate SOHO and interplanetary detections of hot plasma. SOHO/UVCS has found evidence for very hot coronal plasma in current sheets in the aftermath of CMEs in the [Fe XVIII] $\\lambda$ \\AA\\ line, implying a temperature on the order of $6\\times 10(exp 6)$ K. This temperature is unusually high even for active regions, but is compatible with the high temperature predicted in current sheets. In the solar wind, ACE data from early 1998 to middle 2000 revealed high frozen-in Fe charge state in many cases to be present in interplanetary plasma.

Mission Peculiar Equipment (MPE) For Spacelab Mission 1 Payload

NASA Astrophysics Data System (ADS)

Sims, John H.; Dodeck, Hauke

1982-02-01

Spacelab interfaces and services for payloads are advertised in the Spacelab Payload Accommodations Handbook (SPAH). These accommodations are available to the total payload and must be managed and apportioned by a payload integrator. A major part of the integration task is satisfying all instruments/facilities servicing requirements which vary with each item of payload equipment and, when totalled, sometimes exceed the capabilities as defined in SPAH. Such a determination is an output of the integrated payload design and integration effort which consists of analytical assessments based on individual payload equipment requirements inputs, STS and Spacelab available accommodations and constraints, and programmatic considerations. This systems engineering activity spans all engineering disciplines, assesses the module and pallet layouts and simultaneous operation of instrument/facility combinations, and requires a detailed knowledge of the Spacelab design. Introduction of a broad range of payload integrator-provided Mission Peculiar Equipment (MPE) into the Spacelab Mission 1 payload complement was necessary to be added to the Spacelab provisions in order to satisfy the interface and service requirements for each payload developer. This paper provides insight into various aspects of this MPE; including why it is needed, driving design considerations, design and development problems, and conclusions and recommendations for the future. MPE identified for Spacelab Mission 1 begins an inventory that will continue to expand as other mission requirements are identified and the Spacelab flight frequency increases.

The quality of life of older people aging in place: a literature review.

PubMed

Vanleerberghe, Patricia; De Witte, Nico; Claes, Claudia; Schalock, Robert L; Verté, Dominique

2017-11-01

In order to cope with the challenges that are the result of an aging population, policies and services promote keeping elders in the community and letting them age in place rather than sending them to specialized institutions. Aging in place refers to the option where people can stay in their homes as they age. This policy option, however, poses various challenges and may also threaten the quality of life of the aging. A literature review was performed on the quality of life of older people aging in place to determine whether the actual assessment of quality of life can be used within aging in place. Web of Science, PubMed, CINAHL, Sociological Abstracts and Social Science Research Network were searched for publications on "Ag(e)ing in place" AND "Quality of life." Although assessment is crucial to a policy pursuing a good quality of life, literature reveals that it is seldom performed. Only a small part of the studies report on the assessment of quality of life, including the instruments used and the results. The findings also indicate that there is no consensus on the definition of quality of life or its domains structures. As no existing instrument assessing the quality of life of older people aging in place could be identified, such a tool should be developed, because any policy towards this growing group of people should be complemented by an evaluation.

Venus Atmospheric Maneuverable Platform Science Mission

NASA Astrophysics Data System (ADS)

Polidan, Ronald S.; Lee, Gregory; Ross, Floyd; Sokol, Daniel; Bolisay, Linden

2015-11-01

Over the past several years, we have explored a possible new approach to Venus upper atmosphere exploration by applying recent Northrop (non-NASA) development programs and have come up with a new class of exploration vehicle: an atmospheric rover. We will discuss a possible suite of instruments and measurements to study the current climate through detailed characterization of cloud level atmosphere and to understand the processes that control climate on Earth-like planets.Our Venus atmospheric rover concept, the Venus Atmospheric Maneuverable Platform (VAMP), is a hypersonic entry vehicle with an ultra-low ballistic coefficient that transitions to a semi-buoyant air vehicle (AV) after entering the Venus atmosphere. Prior to entry, the AV fully deploys to enable lifting entry and eliminates the need for an aeroshell. The mass savings realized by eliminating the aeroshell allows VAMP to accommodate significantly more instruments compared to previous Venus in situ exploration missions. VAMP targets the global Venus atmosphere between 50-65 km altitudes and would be an ideal, stable platform for atmospheric and surface interaction measurements. We will present a straw man concept of VAMP, including its science instrument accommodation capability and platform’s physical characteristics (mass, power, wingspan, etc). We will discuss the various instrument options.VAMP’s subsonic flight regime starts at ~94 km and after <1 hour, the AV will reach its cruise altitude of ~65 km. During this phase of flight, the VAMP sensor suite will acquire a pre-defined set of upper atmosphere measurements. The nominal VAMP lifetime at cruise altitude is several months to a year, providing numerous circumnavigation cycles of Venus at mid-latitude. The stability of the AV and its extended residence time provide the very long integration times required for isotopic mass analysis. VAMP communicates with the orbiter, which provides data relay and possibly additional science measurements complementing the in situ measurements from the AV. We will specifically focus upon key factors impacting the design and performance of VAMP science.

Installation of EarthScope Borehole Strainmeters in Turkey to complement GONAF.

NASA Astrophysics Data System (ADS)

Johnson, W.; Gottlieb, M. H.; Mencin, D.; Van Boskirk, E.; Ozener, H.; Bohnhoff, M.; Bulut, F.; Bal, O.; Acarel, D.; Aydin, H.; Mattioli, G. S.

2015-12-01

Twice in the past 1000 years a sequence of damaging earthquakes has propagated over a period of a few decades along the North Anatolian Fault (NAF) in Turkey towards Istanbul, with the final earthquake in the sequence catastrophically damaging the city. This occurred most recently in 1509, causing 10,000 casualties in a population of about 200,000. The population is now 20 million, the building stock more fragile, and the last earthquake of the current sequence is considered imminent. Since July 2014, UNAVCO has installed 2 EarthScope borehole geophysical instrument strings, which include Gladwin Tensor strainmeters and passive, short-period 3-component seismometers, into boreholes provided by internationally supported Geophysical Observatory at the North Anatolian Fault (GONAF) and Bogazici University Kandilli Observatory. Funding for instruments and staff participation was provided by NSF. If the project remains on schedule, we anticipate that 4 additional BSM strings will be installed by the fall 2015. Our joint international project gives an opportunity to enhance the detection capability of a suite of deep seismometers (GONAF) installed near Istanbul and will permit us to image dynamic rupture along the NAF and to monitor and better understand the tectonic processes leading to failure. The tectonic and geodynamic environment of the NAF near Istanbul in many ways resembles the San Andreas Fault setting of San Francisco; these instruments will enhance the ability to monitor ultra-slow process near the probable source zone of the Mw>7 earthquake beneath the Marmara Sea on the NAF This project has provided UNAVCO an opportunity to gain experience in strainmeters installations outside of North America. The techniques developed to adapt to the challenges of installing borehole strainmeters on islands and other remote locations with limited resources will greatly enhance our ability to install these BSM instruments in similar locations in the future.

Relations between Mental Verb and False Belief Understanding in Cantonese-Speaking Children

ERIC Educational Resources Information Center

Cheung, Him; Chen, Hsuan-Chih; Yeung, William

2009-01-01

Previous research has shown that linguistic forms that codify mental contents bear a specific relation with children's false belief understanding. These forms include mental verbs and their following complements, yet the two have not been considered separately. The current study examined the roles of mental verb semantics and the complement syntax…

Summary Report of Mission Acceleration Measurements for STS-65, Launched 8 July 1994

NASA Technical Reports Server (NTRS)

Rogers, Melissa J. B.; Delombard, Richard

1995-01-01

The second flight of the International Microgravity Laboratory (IML-2) payload on board the STS-65 mission was supported by three accelerometer instruments: The Orbital Acceleration Research Experiment (OARE) located close to the orbiter center of mass; the Quasi-Steady Acceleration Measurement experiment, and the Space Acceleration Measurement System (SAMS), both in the Spacelab module. A fourth accelerometer, the Microgravity Measuring Device recorded data in the middeck in support of exercise isolation tests.Data collected by OARE and SAMS during IML-2 are displayed in this report. The OARE data represent the microgravity environment below 1 Hz. The SAMS data represent the environment in the 0.01 Hz to 100 Hz range. Variations in the environment caused by unique activities are presented. Specific events addressed are: crew activity, crew exercise, experiment component mixing activities, experiment centrifuge operations, refrigerator/freezer operations and circulation pump operations. The analyses included in this report complement analyses presented in other mission summary reports.

ART: Surveying the Local Universe at 2-11 keV

NASA Technical Reports Server (NTRS)

O'Dell, S. L.; Ramsey, B. D.; Adams, M. L.; Brandt, W. N.; Bubarev, M. V.; Hassinger, G.; Pravlinski, M.; Predehl, P.; Romaine, S. E.; Swartz, D. A.;

Service profiling and outcomes benchmarking using the CORE-OM: toward practice-based evidence in the psychological therapies. Clinical Outcomes in Routine Evaluation-Outcome Measures.

PubMed

Barkham, M; Margison, F; Leach, C; Lucock, M; Mellor-Clark, J; Evans, C; Benson, L; Connell, J; Audin, K; McGrath, G

2001-04-01

To complement the evidence-based practice paradigm, the authors argued for a core outcome measure to provide practice-based evidence for the psychological therapies. Utility requires instruments that are acceptable scientifically, as well as to service users, and a coordinated implementation of the measure at a national level. The development of the Clinical Outcomes in Routine Evaluation-Outcome Measure (CORE-OM) is summarized. Data are presented across 39 secondary-care services (n = 2,710) and within an intensively evaluated single service (n = 1,455). Results suggest that the CORE-OM is a valid and reliable measure for multiple settings and is acceptable to users and clinicians as well as policy makers. Baseline data levels of patient presenting problem severity, including risk, are reported in addition to outcome benchmarks that use the concept of reliable and clinically significant change. Basic quality improvement in outcomes for a single service is considered.

Overview and first results of the Wind and Storms Experiment (WASTEX): a field campaign to observe the formation of gusts using a Doppler lidar

NASA Astrophysics Data System (ADS)

Pantillon, Florian; Wieser, Andreas; Adler, Bianca; Corsmeier, Ulrich; Knippertz, Peter

2018-05-01

Wind gusts are responsible for most damages in winter storms over central Europe, but capturing their small scale and short duration is a challenge for both models and observations. This motivated the Wind and Storms Experiment (WASTEX) dedicated to investigate the formation of gusts during the passage of extratropical cyclones. The field campaign took place during the winter 2016-2017 on a former waste deposit located close to Karlsruhe in the Upper Rhine Valley in southwest Germany. Twelve extratropical cyclones were sampled during WASTEX with a Doppler lidar system performing vertical scans in the mean wind direction and complemented with a Doppler C-band radar and a 200 m instrumented tower. First results are provided here for the three most intense storms and include a potential sting jet, a unique direct observation of a convective gust and coherent boundary-layer structures of strong winds.

Earth Science Observations from the International Space Station: An Overview (Invited)

NASA Astrophysics Data System (ADS)

Kaye, J. A.

2013-12-01

The International Space Station (ISS) provides a unique and valuable platform for observing the Earth. With its mid-inclination (~51 degree) orbit, it provides the opportunity to view most of the Earth, with data acquisition possible over a full range of local times, in an orbit that nicely complements the polar sun-synchronous orbits used for much of space-based Earth observation, and can draw on a heritage of mid-inclination observations from both free flying satellites and the Space Shuttle program. The ISS, including its component observing modules supplied by NASA's international partners, can provide needed resources and viewing opportunities by a broad range of Earth-viewing scientific instruments. In this talk, the overall picture of Earth viewing from ISS will be presented, with examples from a range of past, current, and projected sensors being shared; talks on the ISS implementation for a subset of current and projected payload will be presented in individual talks presented by their their respective teams.

A SPECT system simulator built on the SolidWorks TM 3D-Design package.

PubMed

Li, Xin; Furenlid, Lars R

2014-08-17

We have developed a GPU-accelerated SPECT system simulator that integrates into instrument-design workflow [1]. This simulator includes a gamma-ray tracing module that can rapidly propagate gamma-ray photons through arbitrary apertures modeled by SolidWorks TM -created stereolithography (.STL) representations with a full complement of physics cross sections [2, 3]. This software also contains a scintillation detector simulation module that can model a scintillation detector with arbitrary scintillation crystal shape and light-sensor arrangement. The gamma-ray tracing module enables us to efficiently model aperture and detector crystals in SolidWorks TM and save them as STL file format, then load the STL-format model into this module to generate list-mode results of interacted gamma-ray photon information (interaction positions and energies) inside the detector crystals. The Monte-Carlo scintillation detector simulation module enables us to simulate how scintillation photons get reflected, refracted and absorbed inside a scintillation detector, which contributes to more accurate simulation of a SPECT system.

Cardiac Rehabilitation Online Pilot: Extending Reach of Cardiac Rehabilitation.

PubMed

Higgins, Rosemary O; Rogerson, Michelle; Murphy, Barbara M; Navaratnam, Hema; Butler, Michael V; Barker, Lauren; Turner, Alyna; Lefkovits, Jeffrey; Jackson, Alun C

While cardiac rehabilitation (CR) is recommended for all patients after an acute cardiac event, limitations exist in reach. The purpose of the current study was to develop and pilot a flexible online CR program based on self-management principles "Help Yourself Online." The program was designed as an alternative to group-based CR as well as to complement traditional CR. The program was based on existing self-management resources developed previously by the Heart Research Centre. Twenty-one patients admitted to Cabrini Health for an acute cardiac event were recruited to test the program. The program was evaluated using qualitative and quantitative methods. Quantitative results demonstrated that patients believed the program would assist them in their self-management. Qualitative evaluation, using focus group and interview methods with 15 patients, showed that patients perceived the online CR approach to be a useful instrument for self-management. Broader implications of the data include the acceptability of the intervention, timing of intervention delivery, and patients' desire for additional online community support.

Analyses of cosmic ray induced-neutron based on spectrometers operated simultaneously at mid-latitude and Antarctica high-altitude stations during quiet solar activity

NASA Astrophysics Data System (ADS)

Hubert, G.

2016-10-01

In this paper are described a new neutron spectrometer which operate in the Concordia station (Antarctica, Dome C) since December 2015. This instrument complements a network including neutron spectrometers operating in the Pic-du-Midi and the Pico dos Dias. Thus, this work present an analysis of cosmic ray induced-neutron based on spectrometers operated simultaneously in the Pic-du-Midi and the Concordia stations during a quiet solar activity. The both high station platforms allow for investigating the long period dynamics to analyze the spectral variation and effects of local and seasonal changes, but also the short term dynamics during solar flare events. A first part is devoted to analyze the count rates, the spectrum and the neutron fluxes, implying cross-comparisons between data obtained in the both stations. In a second part, measurements analyses were reinforced by modeling based on simulations of atmospheric cascades according to primary spectra which only depend on the solar modulation potential.

Characterization of the cloud conditions at Ny-Ålesund using sensor synergy and representativeness of the observed clouds across Arctic sites

NASA Astrophysics Data System (ADS)

Nomokonova, Tatiana; Ebell, Kerstin; Löhnert, Ulrich; Maturilli, Marion

2017-04-01

Clouds are one of the crucial components of the hydrological and energy cycles and thus affecting the global climate. Their special importance in Arctic regions is defined by cloud's influence on the radiation budget. Arctic clouds usually occur at low altitudes and often contain highly concentrated tiny liquid drops. During winter, spring, and autumn periods such clouds tend to conserve the long-wave radiation in the atmosphere and, thus, produce warming of the Arctic climate. In summer though clouds efficiently scatter the solar radiation back to space and, therefore, induce a cooling effect. An accurate characterization of the net effect of clouds on the Arctic climate requires long-term and precise observations. However, only a few measurement sites exist which perform continuous, vertically resolved observations of clouds in the Arctic, e.g. in Alaska, Canada, and Greenland. These sites typically make use of a combination of different ground-based remote sensing instruments, e.g. cloud radar, ceilometer and microwave radiometer in order to characterize clouds. Within the Transregional Collaborative Research Center (TR 172) "Arctic Amplification: Climate Relevant Atmospheric and Surface Processes, and Feedback Mechanisms (AC)3" comprehensive observations of the atmospheric column are performed at the German-French Research Station AWIPEV at Ny-Ålesund, Svalbard. Ny-Ålesund is located in the warmest part of the Arctic where climate is significantly influenced by adiabatic heating from the warm ocean. Thus, measurements at Ny-Ålesund will complement our understanding of cloud formation and development in the Arctic. This particular study is devoted to the characterization of the cloud macro- and microphysical properties at Ny-Ålesund and of the atmospheric conditions, under which these clouds form and develop. To this end, the information of the various instrumentation at the AWIPEV observatory is synergistically analysed: information about the thermodynamic structure of the atmosphere is obtained from long-term radiosonde launches. In addition, continuous vertical profiles of temperature and humidity are provided by the microwave radiometer HATPRO. A set of active remote sensing instruments performs cloud observations at Ny-Ålesund: a ceilometer and a Doppler lidar operating since 2011 and 2013, respectively, are now complemented with a novel 94 GHz FMCW cloud radar. As a first step, the CLOUDNET algorithms, including a target categorization and classification, are applied to the observations. In this study, we will present a first analysis of cloud properties at Ny-Ålesund including for example cloud occurrence, cloud geometry (cloud base, cloud top, and thickness) and cloud type (liquid, ice, mixed-phase). The different types of clouds are set into context to the environmental conditions such as temperature, amount of water vapour, and liquid water. We also expect that the cloud properties strongly depend on the wind direction. The first results of this analysis will be also shown.

Isotopes in the Arctic atmospheric water cycle

NASA Astrophysics Data System (ADS)

Bonne, Jean-Louis; Werner, Martin; Meyer, Hanno; Kipfstuhl, Sepp; Rabe, Benjamin; Behrens, Melanie; Schönicke, Lutz; Steen Larsen, Hans Christian; Masson-Delmotte, Valérie

2016-04-01

The ISO-ARC project aims at documenting the Arctic atmospheric hydrological cycle, by assessing the imprint of the marine boundary conditions (e.g. temperature variations, circulation changes, or meltwater input) to the isotopic composition of the atmospheric water cycle (H218O and HDO) with a focus on North Atlantic and Arctic oceans. For this purpose, two continuous monitoring water vapour stable isotopes cavity ring-down spectrometers have been installed in July 2015: on-board the Polarstern research vessel and in the Siberian Lena delta Samoylov research station (N 72° 22', E 126° 29'). The Polarstern measurements cover the summer 2015 Arctic campaign from July to mid-October, including six weeks in the Fram Strait region in July- August, followed by a campaign reaching the North Pole and a transect from the Norwegian Sea to the North Sea. These vapour observations are completed by water isotopic measurements in samples from the surface ocean water for Polarstern and from precipitation in Samoylov and Tiksi (120 km south-east of the station). A custom-made designed automatic calibration system has been implemented in a comparable manner for both vapour instruments, based on the injection of different liquid water standards, which are completely vaporised in dry air at high temperature. Subsequent humidity level can be adjusted from 2000 to at least 30000 ppm. For a better resilience, an independent calibration system has been added on the Samoylov instrument, allowing measurements of one standard at humidity levels ranging from 2000 to 15000 ppm: dry air is introduced in a tank containing a large amount of liquid water standard, undergoing evaporation under a controlled environment. The measurement protocol includes an automatic calibration every 25 hours. First instrument characterisation experiments depict a significant isotope-humidity effect at low humidity, dependant on the isotopic composition of the standard. For ambient air, our first isotope measurements highlighted significant synoptic variations in summer in both sites. In Samoylov, the premises of a seasonal cycle have been observed during the summer-fall transition, with a fast humidity level and isotopic decrease. The latitudinal gradient is also highly visible on the Polarstern record. Complementing simulations of the water vapour isotopic composition by the ECHAM5-wiso model reproduce the Polarstern synoptic variability and spatial patterns with a good accuracy. In the near future, our records will be combined with simultaneous water isotope measurements in Iceland (by Hans Christian Steen-Larsen, CIC), Svalbard (by Valérie-Masson Delmotte, LSCE) and paired with complementing climate simulations enhanced by water isotope diagnostics. These data sets, covering an approx. 6,000 km transect of Northern Eurasia will allow for a quantitative assessment of the isotopic variations of the Arctic water cycle. The results of these analyses will also be of relevance for the interpretation of isotope signals found in ice cores and on terrestrial Arctic sites in terms of past climate change.

The Lectin Pathway of Complement and Rheumatic Heart Disease

PubMed Central

Beltrame, Marcia Holsbach; Catarino, Sandra Jeremias; Goeldner, Isabela; Boldt, Angelica Beate Winter; de Messias-Reason, Iara José

2014-01-01

The innate immune system is the first line of host defense against infection and is comprised of humoral and cellular mechanisms that recognize potential pathogens within minutes or hours of entry. The effector components of innate immunity include epithelial barriers, phagocytes, and natural killer cells, as well as cytokines and the complement system. Complement plays an important role in the immediate response against microorganisms, including Streptococcus sp. The lectin pathway is one of three pathways by which the complement system can be activated. This pathway is initiated by the binding of mannose-binding lectin (MBL), collectin 11 (CL-K1), and ficolins (Ficolin-1, Ficolin-2, and Ficolin-3) to microbial surface oligosaccharides and acetylated residues, respectively. Upon binding to target molecules, MBL, CL-K1, and ficolins form complexes with MBL-associated serine proteases 1 and 2 (MASP-1 and MASP-2), which cleave C4 and C2 forming the C3 convertase (C4b2a). Subsequent activation of complement cascade leads to opsonization, phagocytosis, and lysis of target microorganisms through the formation of the membrane-attack complex. In addition, activation of complement may induce several inflammatory effects, such as expression of adhesion molecules, chemotaxis and activation of leukocytes, release of reactive oxygen species, and secretion of cytokines and chemokines. In this chapter, we review the general aspects of the structure, function, and genetic polymorphism of lectin-pathway components and discuss most recent understanding on the role of the lectin pathway in the predisposition and clinical progression of Rheumatic Fever. PMID:25654073

Kinematic Validation of a Multi-Kinect v2 Instrumented 10-Meter Walkway for Quantitative Gait Assessments.

PubMed

Geerse, Daphne J; Coolen, Bert H; Roerdink, Melvyn

2015-01-01

Walking ability is frequently assessed with the 10-meter walking test (10MWT), which may be instrumented with multiple Kinect v2 sensors to complement the typical stopwatch-based time to walk 10 meters with quantitative gait information derived from Kinect's 3D body point's time series. The current study aimed to evaluate a multi-Kinect v2 set-up for quantitative gait assessments during the 10MWT against a gold-standard motion-registration system by determining between-systems agreement for body point's time series, spatiotemporal gait parameters and the time to walk 10 meters. To this end, the 10MWT was conducted at comfortable and maximum walking speed, while 3D full-body kinematics was concurrently recorded with the multi-Kinect v2 set-up and the Optotrak motion-registration system (i.e., the gold standard). Between-systems agreement for body point's time series was assessed with the intraclass correlation coefficient (ICC). Between-systems agreement was similarly determined for the gait parameters' walking speed, cadence, step length, stride length, step width, step time, stride time (all obtained for the intermediate 6 meters) and the time to walk 10 meters, complemented by Bland-Altman's bias and limits of agreement. Body point's time series agreed well between the motion-registration systems, particularly so for body points in motion. For both comfortable and maximum walking speeds, the between-systems agreement for the time to walk 10 meters and all gait parameters except step width was high (ICC ≥ 0.888), with negligible biases and narrow limits of agreement. Hence, body point's time series and gait parameters obtained with a multi-Kinect v2 set-up match well with those derived with a gold standard in 3D measurement accuracy. Future studies are recommended to test the clinical utility of the multi-Kinect v2 set-up to automate 10MWT assessments, thereby complementing the time to walk 10 meters with reliable spatiotemporal gait parameters obtained objectively in a quick, unobtrusive and patient-friendly manner.

Kinematic Validation of a Multi-Kinect v2 Instrumented 10-Meter Walkway for Quantitative Gait Assessments

PubMed Central

Geerse, Daphne J.; Coolen, Bert H.; Roerdink, Melvyn

2015-01-01

Walking ability is frequently assessed with the 10-meter walking test (10MWT), which may be instrumented with multiple Kinect v2 sensors to complement the typical stopwatch-based time to walk 10 meters with quantitative gait information derived from Kinect’s 3D body point’s time series. The current study aimed to evaluate a multi-Kinect v2 set-up for quantitative gait assessments during the 10MWT against a gold-standard motion-registration system by determining between-systems agreement for body point’s time series, spatiotemporal gait parameters and the time to walk 10 meters. To this end, the 10MWT was conducted at comfortable and maximum walking speed, while 3D full-body kinematics was concurrently recorded with the multi-Kinect v2 set-up and the Optotrak motion-registration system (i.e., the gold standard). Between-systems agreement for body point’s time series was assessed with the intraclass correlation coefficient (ICC). Between-systems agreement was similarly determined for the gait parameters’ walking speed, cadence, step length, stride length, step width, step time, stride time (all obtained for the intermediate 6 meters) and the time to walk 10 meters, complemented by Bland-Altman’s bias and limits of agreement. Body point’s time series agreed well between the motion-registration systems, particularly so for body points in motion. For both comfortable and maximum walking speeds, the between-systems agreement for the time to walk 10 meters and all gait parameters except step width was high (ICC ≥ 0.888), with negligible biases and narrow limits of agreement. Hence, body point’s time series and gait parameters obtained with a multi-Kinect v2 set-up match well with those derived with a gold standard in 3D measurement accuracy. Future studies are recommended to test the clinical utility of the multi-Kinect v2 set-up to automate 10MWT assessments, thereby complementing the time to walk 10 meters with reliable spatiotemporal gait parameters obtained objectively in a quick, unobtrusive and patient-friendly manner. PMID:26461498

Devices and tasks involved in the objective assessment of standing dynamic balancing - A systematic literature review.

PubMed

Petró, Bálint; Papachatzopoulou, Alexandra; Kiss, Rita M

2017-01-01

Static balancing assessment is often complemented with dynamic balancing tasks. Numerous dynamic balancing assessment methods have been developed in recent decades with their corresponding balancing devices and tasks. The aim of this systematic literature review is to identify and categorize existing objective methods of standing dynamic balancing ability assessment with an emphasis on the balancing devices and tasks being used. Three major scientific literature databases (Science Direct, Web of Science, PLoS ONE) and additional sources were used. Studies had to use a dynamic balancing device and a task described in detail. Evaluation had to be based on objectively measureable parameters. Functional tests without instrumentation evaluated exclusively by a clinician were excluded. A total of 63 articles were included. The data extracted during full-text assessment were: author and date; the balancing device with the balancing task and the measured parameters; the health conditions, size, age and sex of participant groups; and follow-up measurements. A variety of dynamic balancing assessment devices were identified and categorized as 1) Solid ground, 2) Balance board, 3) Rotating platform, 4) Horizontal translational platform, 5) Treadmill, 6) Computerized Dynamic Posturography, and 7) Other devices. The group discrimination ability of the methods was explored and the conclusions of the studies were briefly summarized. Due to the wide scope of this search, it provides an overview of balancing devices and do not represent the state-of-the-art of any single method. The identified dynamic balancing assessment methods are offered as a catalogue of candidate methods to complement static assessments used in studies involving postural control.

Considerations for pattern placement error correction toward 5nm node

NASA Astrophysics Data System (ADS)

Yaegashi, Hidetami; Oyama, Kenichi; Hara, Arisa; Natori, Sakurako; Yamauchi, Shohei; Yamato, Masatoshi; Koike, Kyohei; Maslow, Mark John; Timoshkov, Vadim; Kiers, Ton; Di Lorenzo, Paolo; Fonseca, Carlos

2017-03-01

Multi-patterning has been adopted widely in high volume manufacturing as 193 immersion extension, and it becomes realistic solution of nano-order scaling. In fact, it must be key technology on single directional (1D) layout design [1] for logic devise and it becomes a major option for further scaling technique in SAQP. The requirement for patterning fidelity control is getting savior more and more, stochastic fluctuation as well as LER (Line edge roughness) has to be micro-scopic observation aria. In our previous work, such atomic order controllability was viable in complemented technique with etching and deposition [2]. Overlay issue form major potion in yield management, therefore, entire solution is needed keenly including alignment accuracy on scanner and detectability on overlay measurement instruments. As EPE (Edge placement error) was defined as the gap between design pattern and contouring of actual pattern edge, pattern registration in single process level must be considerable. The complementary patterning to fabricate 1D layout actually mitigates any process restrictions, however, multiple process step, symbolized as LELE with 193-i, is burden to yield management and affordability. Recent progress of EUV technology is remarkable, and it is major potential solution for such complicated technical issues. EUV has robust resolution limit and it must be definitely strong scaling driver for process simplification. On the other hand, its stochastic variation such like shot noise due to light source power must be resolved with any additional complemented technique. In this work, we examined the nano-order CD and profile control on EUV resist pattern and would introduce excellent accomplishments.

Fast Plasma Instrument for MMS: Data Compression Simulation Results

NASA Technical Reports Server (NTRS)

Barrie, A.; Adrian, Mark L.; Yeh, P.-S.; Winkert, G. E.; Lobell, J. V.; Vinas, A.F.; Simpson, D. J.; Moore, T. E.

2008-01-01

Magnetospheric Multiscale (MMS) mission will study small-scale reconnection structures and their rapid motions from closely spaced platforms using instruments capable of high angular, energy, and time resolution measurements. To meet these requirements, the Fast Plasma Instrument (FPI) consists of eight (8) identical half top-hat electron sensors and eights (8) identical ion sensors and an Instrument Data Processing Unit (IDPU). The sensors (electron or ion) are grouped into pairs whose 6 deg x 180 deg fields-of-view (FOV) are set 90 deg apart. Each sensor is equipped with electrostatic aperture steering to allow the sensor to scan a 45 deg x 180 deg fan about its nominal viewing (0 deg deflection) direction. Each pair of sensors, known as the Dual Electron Spectrometer (DES) and the Dual Ion Spectrometer (DIS), occupies a quadrant on the MMS spacecraft and the combination of the eight electron/ion sensors, employing aperture steering, image the full-sky every 30-ms (electrons) and 150-ms (ions), respectively. To probe the results in the DES complement of a given spacecraft generating 6.5-Mbs(exp -1) of electron data while the DIS generates 1.1-Mbs(exp -1) of ion data yielding an FPI total data rate of 6.6-MBs(exp -1). The FPI electron/ion data is collected by the IDPU then transmitted to the Central Data Instrument Processor (CIDP) on the spacecraft for science interest ranking. Only data sequences that contain the greatest amount of temporal/spatial structure will be intelligently down-linked by the spacecraft. Currently, the FPI data rate allocation to the CIDP is 1.5-Mbs(exp -1). Consequently, the FPI-IDPU must employ data/image compression to meet this CIDP telemetry allocation. Here, we present simulations of the CCSDS 122.0-B-1 algorithm-based compression of the FPI-DES electron data. Compression analysis is based upon a seed of re-processed Cluster/PEACE electron measurements. Topics to be discussed include: review of compression algorithm; data quality; data formatting/organization; and, implications for data/matrix pruning. To conclude a presentation of the base-lined FPI data compression approach is provided.

Advances in Satellite Remote Sensing of Particulate Air Pollution: From MISR to MAIA

NASA Astrophysics Data System (ADS)

Diner, D. J.; Burke, K.; Xu, F.; Garay, M. J.; Kalashnikova, O. V.; Liu, Y.; Meng, X.; Wang, J.; Martin, R.; Ostro, B.

2017-12-01

Airborne particulate matter (PM) is a well-known cause of cardiovascular and respiratory disease. To estimate human exposure to PM pollution, satellite instruments such as the Terra Multi-angle Imaging SpectroRadiometer (MISR) and the Moderate resolution Imaging Spectroradiometer (MODIS) have been used in conjunction with surface monitors to map near-surface PM concentrations. The relative toxicity of different size and compositional mixtures of PM is not well understood. To address this, we are developing the Multi-Angle Imager for Aerosols (MAIA) investigation. The satellite instrument extends MISR's multiangular visible and near-infrared (VNIR) spectral coverage to 14 bands in the ultraviolet, VNIR, and shortwave IR; three of the bands are polarimetric to enhance sensitivity to aerosol size and composition. To constrain the retrievals, the observations will be combined with data from surface monitors and the WRF-Chem and GEOS-Chem chemical transport models. Existing surface PM speciation monitors will be supplemented by adding new stations to the Surface PARTiculate mAtter Network (SPARTAN). Unlike MISR, MAIA is a targeting instrument. Primary areas of interest include metropolitan areas in North and South America, Europe, the Middle East, Africa, India, and East Asia. PM retrieval algorithms are being developed using data from MISR and the high-altitude Airborne Multiangle SpectroPolarimetric Imager (AirMSPI). Epidemiologists on the MAIA science team will use the derived PM data products and birth, death, and hospital records to investigate adverse health impacts of different types of airborne particulates. MAIA's earliest possible launch date is mid-2020, making it possible for the data to be complemented by global observations from Terra as well as high temporal resolution atmospheric chemistry measurements from TEMPO (Tropospheric Emissions: Monitoring Pollution), GEMS (Geostationary Environment Monitoring Spectrometer), and Sentinel-4.

Status of the Stratospheric Observatory for Infrared Astronomy (SOFIA)

NASA Astrophysics Data System (ADS)

Gehrz, R. D.; Becklin, E. E.; de Buizer, J.; Herter, T.; Keller, L. D.; Krabbe, A.; Marcum, P. M.; Roellig, T. L.; Sandell, G. H. L.; Temi, P.; Vacca, W. D.; Young, E. T.; Zinnecker, H.

2011-09-01

The Stratospheric Observatory for Infrared Astronomy (SOFIA), a joint US/German project, is a 2.5-m infrared airborne telescope carried by a Boeing 747-SP that flies in the stratosphere at altitudes as high as 45,000 ft (13.72 km). This facility is capable of observing from 0.3 μm to 1.6 mm with an average transmission greater than 80% averaged over all wavelengths. SOFIA will be staged out of the NASA Dryden Flight Research Center aircraft operations facility at Palmdale, CA. The SOFIA Science Mission Operations (SMO) will be located at NASA Ames Research Center, Moffett Field, CA. First science flights began in 2010 and a full operations schedule of up to one hundred 8 to 10 hour-long flights per year will be reached by 2014. The observatory is expected to operate until the mid-2030s. SOFIA's initial complement of seven focal plane instruments includes broadband imagers, moderate-resolution spectrographs that will resolve broad features due to dust and large molecules, and high-resolution spectrometers capable of studying the kinematics of atomic and molecular gas at sub-km/s resolution. We describe the SOFIA facility and outline the opportunities for observations by the general scientific community and for future instrumentation development. The operational characteristics of the SOFIA first-generation instruments are summarized. The status of the flight test program is discussed and we show First Light images obtained at wavelengths from 5.4 to 37 μm with the FORCAST imaging camera. Additional information about SOFIA is available at http://www.sofia.usra.edu and http://www.sofia.usra.edu/Science/docs/SofiaScienceVision051809-1.pdf.

Socio-demographic, clinical characteristics and utilization of mental health care services associated with SF-6D utility scores in patients with mental disorders: contributions of the quantile regression.

PubMed

Prigent, Amélie; Kamendje-Tchokobou, Blaise; Chevreul, Karine

2017-11-01

Health-related quality of life (HRQoL) is a widely used concept in the assessment of health care. Some generic HRQoL instruments, based on specific algorithms, can generate utility scores which reflect the preferences of the general population for the different health states described by the instrument. This study aimed to investigate the relationships between utility scores and potentially associated factors in patients with mental disorders followed in inpatient and/or outpatient care settings using two statistical methods. Patients were recruited in four psychiatric sectors in France. Patient responses to the SF-36 generic HRQoL instrument were used to calculate SF-6D utility scores. The relationships between utility scores and patient socio-demographic, clinical characteristics, and mental health care utilization, considered as potentially associated factors, were studied using OLS and quantile regressions. One hundred and seventy six patients were included. Women, severely ill patients and those hospitalized full-time tended to report lower utility scores, whereas psychotic disorders (as opposed to mood disorders) and part-time care were associated with higher scores. The quantile regression highlighted that the size of the associations between the utility scores and some patient characteristics varied along with the utility score distribution, and provided more accurate estimated values than OLS regression. The quantile regression may constitute a relevant complement for the analysis of factors associated with utility scores. For policy decision-making, the association of full-time hospitalization with lower utility scores while part-time care was associated with higher scores supports the further development of alternatives to full-time hospitalizations.

PACIC Instrument: disentangling dimensions using published validation models.

PubMed

Iglesias, K; Burnand, B; Peytremann-Bridevaux, I

2014-06-01

To better understand the structure of the Patient Assessment of Chronic Illness Care (PACIC) instrument. More specifically to test all published validation models, using one single data set and appropriate statistical tools. Validation study using data from cross-sectional survey. A population-based sample of non-institutionalized adults with diabetes residing in Switzerland (canton of Vaud). French version of the 20-items PACIC instrument (5-point response scale). We conducted validation analyses using confirmatory factor analysis (CFA). The original five-dimension model and other published models were tested with three types of CFA: based on (i) a Pearson estimator of variance-covariance matrix, (ii) a polychoric correlation matrix and (iii) a likelihood estimation with a multinomial distribution for the manifest variables. All models were assessed using loadings and goodness-of-fit measures. The analytical sample included 406 patients. Mean age was 64.4 years and 59% were men. Median of item responses varied between 1 and 4 (range 1-5), and range of missing values was between 5.7 and 12.3%. Strong floor and ceiling effects were present. Even though loadings of the tested models were relatively high, the only model showing acceptable fit was the 11-item single-dimension model. PACIC was associated with the expected variables of the field. Our results showed that the model considering 11 items in a single dimension exhibited the best fit for our data. A single score, in complement to the consideration of single-item results, might be used instead of the five dimensions usually described. © The Author 2014. Published by Oxford University Press in association with the International Society for Quality in Health Care; all rights reserved.

Elucidating unconscious processing with instrumental hypnosis

PubMed Central

Landry, Mathieu; Appourchaux, Krystèle; Raz, Amir

2014-01-01

Most researchers leverage bottom-up suppression to unlock the underlying mechanisms of unconscious processing. However, a top-down approach – for example via hypnotic suggestion – paves the road to experimental innovation and complementary data that afford new scientific insights concerning attention and the unconscious. Drawing from a reliable taxonomy that differentiates subliminal and preconscious processing, we outline how an experimental trajectory that champions top-down suppression techniques, such as those practiced in hypnosis, is uniquely poised to further contextualize and refine our scientific understanding of unconscious processing. Examining subliminal and preconscious methods, we demonstrate how instrumental hypnosis provides a reliable adjunct that supplements contemporary approaches. Specifically, we provide an integrative synthesis of the advantages and shortcomings that accompany a top-down approach to probe the unconscious mind. Our account provides a larger framework for complementing the results from core studies involving prevailing subliminal and preconscious techniques. PMID:25120504

Turning men into machines? Scientific management, industrial psychology, and the "human factor".

PubMed

Derksen, Maarten

2014-01-01

In the controversy that broke out in 1911 over Frederick W. Taylor's scientific management, many critics contended that it ignored "the human factor" and reduced workers to machines. Psychologists succeeded in positioning themselves as experts of the human factor, and their instruments and expertise as the necessary complement of Taylor's psychologically deficient system. However, the conventional view that the increasing influence of psychologists and other social scientists "humanized" management theory and practice needs to be amended. Taylor's scientific management was not less human than later approaches such as Human Relations, but it articulated the human factor differently, and aligned it to its own instruments and practices in such a way that it was at once external to them and essential to their functioning. Industrial psychologists, on the other hand, at first presented themselves as engineers of the human factor and made the human mind an integral part of management. © 2014 Wiley Periodicals, Inc.

[The Munich Attachment and Effectiveness Study: Study Design and a Case Example].

PubMed

Hörz-Sagstetter, Susanne; Minow, Anna-Rahel; Erhardt, Ingrid; Barten-Hohn, Julia; Denscherz, Claudia; Buchheim, Anna; Taubner, Svenja; Mertens, Wolfgang

2016-03-01

The "Munich Attachment and Effectiveness Study" is a prospective psychotherapy study examining process and outcome of psychoanalytic psychotherapies. The study design and results are exemplified in a single case. At 6 points in time audio-taped and transcribed therapy sessions are evaluated using process instruments (e. g. Psychotherapy Process Q-Set PQS) and interviews (e. g. Operationalized Psychodynamic Diagnostics OPD, Heidelberg Structural Change Scale HSCS, Adult Attachment Interview AAI). In the single case, findings from the psychotherapeutic process (e. g. "therapist is empathic" according to PQS) complement the achieved changes. 5 HSCS problem foci reached level of "restructuring", on the Reflective Functioning Scale a marked change of RF took place and the attachment classification changed over time. The instruments employed in this study corresponded well in assessing change processes in spite of differing theoretical background. © Georg Thieme Verlag KG Stuttgart · New York.

An Overview of the Juno Mission to Jupiter

NASA Technical Reports Server (NTRS)

Grammier, Richard S.

2006-01-01

Arriving in orbit around the planet Jupiter in 2016 after a five-year journey, the Juno spacecraft will begin a one-year investigation of the gas giant in order to understand its origin and evolution by determining its water abundance and constraining its core mass. In addition, Juno will map the planet's magnetic and gravitational fields, map its atmosphere, and explore the three-dimensional structure of Jupiter's polar magnetosphere and auroras. Juno will discriminate among different models for giant planet formation. These investigations will be conducted over the course of thirty-two 11-day elliptical polar orbits of the planet. The orbits are designed to avoid Jupiter's highest radiation regions. The spacecraft is a spinning, solar-powered system carrying a complement of eight science instruments for conducting the investigations. The spacecraft systems and instruments take advantage of significant design and operational heritage from previous space missions.

The new HMI beamline MAGS: an instrument for hard X-ray diffraction at BESSY.

PubMed

Dudzik, Esther; Feyerherm, Ralf; Diete, Wolfgang; Signorato, Riccardo; Zilkens, Christopher

2006-11-01

The Hahn-Meitner-Institute Berlin is operating the new hard X-ray diffraction beamline MAGS at the Berlin synchrotron radiation source BESSY. The beamline is intended to complement the existing neutron instrumentation at the Berlin Neutron Scattering Centre. The new beamline uses a 7 T multipole wiggler to produce photon fluxes in the 10(11)-10(12) photons s(-1) (100 mA)(-1) (0.1% bandwidth)(-1) range at energies from 4 to 30 keV at the experiment. It has active bendable optics to provide flexible horizontal and vertical focusing and to compensate the large heat load from the wiggler source. The experimental end-station consists of a six-circle Huber diffractometer which can be used with an additional (polarization) analyser and different sample environments. The beamline is intended for single-crystal diffraction and resonant magnetic scattering experiments for the study of ordering phenomena, phase transitions and materials science.

Development of the Mars Observer Laser Altimeter (MOLA)

NASA Technical Reports Server (NTRS)

Johnson, Bertrand L., Jr

1993-01-01

The Mars Observer (MO) spacecraft payload scientific mission is to gather data on Martian global topography, gravity, weather, magnetic field and its interaction with the solar flux, surface chemistry, and mineralogy over one Mars year. In mid-1988 the need for a replacement altimeter as part of the payload complement arose. The Mars Observer Laser Altimeter (MOLA) was proposed by GSFC as an in-house effort and shortly afterward was 'conditionally' accepted. Constraints on funding, schedule, power, and mass were imposed with periodic reviews during the instrument development to authorize continuation. MOLA was designed, tested, and delivered in less than 36 months and integrated with the spacecraft. During spacecraft payload testing, the laser failed due to contamination in the laser cavity. In only 6 months, the laser was removed, rebuilt from spare parts, retested, and the instrument reassembled, realigned, requalified, and again delivered for spacecraft integration. Other aspects of the development of the MOLA are presented.

The Open AUC Project.

PubMed

Cölfen, Helmut; Laue, Thomas M; Wohlleben, Wendel; Schilling, Kristian; Karabudak, Engin; Langhorst, Bradley W; Brookes, Emre; Dubbs, Bruce; Zollars, Dan; Rocco, Mattia; Demeler, Borries

2010-02-01

Progress in analytical ultracentrifugation (AUC) has been hindered by obstructions to hardware innovation and by software incompatibility. In this paper, we announce and outline the Open AUC Project. The goals of the Open AUC Project are to stimulate AUC innovation by improving instrumentation, detectors, acquisition and analysis software, and collaborative tools. These improvements are needed for the next generation of AUC-based research. The Open AUC Project combines on-going work from several different groups. A new base instrument is described, one that is designed from the ground up to be an analytical ultracentrifuge. This machine offers an open architecture, hardware standards, and application programming interfaces for detector developers. All software will use the GNU Public License to assure that intellectual property is available in open source format. The Open AUC strategy facilitates collaborations, encourages sharing, and eliminates the chronic impediments that have plagued AUC innovation for the last 20 years. This ultracentrifuge will be equipped with multiple and interchangeable optical tracks so that state-of-the-art electronics and improved detectors will be available for a variety of optical systems. The instrument will be complemented by a new rotor, enhanced data acquisition and analysis software, as well as collaboration software. Described here are the instrument, the modular software components, and a standardized database that will encourage and ease integration of data analysis and interpretation software.

Operations and Data Processing for the Planck Low-Frequency Instrument: Design Strategies and Practical Experience

NASA Astrophysics Data System (ADS)

Pasian, F.; Zacchei, A.; Frailis, M.; Galeotta, S.; Maris, M.; Tavagnacco, D.; Vuerli, C.; Tuerler, M.; Rohlfs, R.; Morisset, N.; Meharga, M.; Ensslin, T. A.; Knoche, J.; Gregorio, A.; Maino, D.; Mennella, A.; Tomasi, M.; Cuttaia, F.; Morgante, G.; Terenzi, L.; Maggio, G.; Gasparo, F.; Franceschi, E.

2012-09-01

Planck is an ESA mission launched in May 2009, which is mapping the microwave sky in nine frequencies and accurately measuring the anisotropies of the Cosmic Microwave Background (CMB) with its complement of two instruments (HFI and LFI), covering respectively the far infrared and the radio domains. The operations and data processing of the Planck instruments are carried out by Data Processing Centers, one for each instrument. The DPCs need to support both a day-by-day quasi-real-time calibration workflow and high-throughput pipelines for a high-volume data flow. The LFI DPC has been designed to be a centralized facility built by geographically distributed institutions, in a funding scenario based on multiple funding agencies and, in most cases, on a fixed budget in the presence of launch delays. A strategy for managing effectively the distributed and collaborative software development and maintenance has been developed, based on the use of open source and off-the-shelf software, and on the reuse of systems developed ad-hoc for other missions. Product and quality assurance has been supported throughout development, integration and testing. The effectiveness of the design choices has been proven by the readiness of the system at launch time and by the extremely smooth operations phase.

Adaptation Strategies to Climate Change and the Role of Planning Instruments - The Example of the Dresden Region (Saxony/Germany)

NASA Astrophysics Data System (ADS)

Albrecht, J.; Juta, K.; Nobis, A.

2009-04-01

In the past, identifying anthropogenic influences on climate change, scenario analyses and issues of climate change mitigation were predominant approaches in climate change research (IPCC 2007). Currently, for instance in Germany, climate impact research on regional level comes to the forefront of research and policy making. Climate change has become an important topic on the agenda of politicians, administration and planning. In order to counteract the (unavoidable) climate change and its impacts it is necessary to develop adaptation strategies. At present, such strategies and guidelines are formulated on international, supranational and national level. The initial point was the United Nations Framework Convention on Climate Change in 1992 where the contracting states obligated themselves to develop national (and regional) programmes for adaptation. In 2007 the European Commission published its Green Paper called Adaptation to Climate Change in Europe. The paper states that adaptation efforts have to be intensified at different (spatial) levels (local, regional, national, and so forth). Furthermore, coordinating these efforts is of high importance. With the recent agreement on the German Adaptation Strategy to Climate Change (DAS 2008) in December 2008, federal government tries to accomplish this task. The German strategy mainly focuses on two elements: decreasing vulnerability and increasing adaptability. While the above mentioned strategies have presented information and policies concerning climate change and adaptation on international, supranational and national level, such documents dońt yet exist on regional level. However, because of their close link to the local level the regions are of high importance for adaptation strategies. Therefore, the Leibniz-Institute of Ecological and Regional Development developed a transdisciplinary project to formulate and implement the so-called Integrated Regional Climate Adaptation Programme (IRCAP) for the Model Region of Dresden (project REGKLAM). The REGKLAM-project is based on regionalised scenarios of climate change and includes measures of climate change adaptation to change for instance, urban form, infrastructure assets (e.g., reservoirs) and land use. Various institutions from politics, administration, economy, and research as well as civil society are involved in the project (the city of Dresden, several ministries and authorities of Saxony, the Dresden Chamber of Industry and Commerce and the University of Dresden). The IRCAP is planned to be an informal, cross-sectoral instrument of adaptation to climate change. As a regional programme, the IRCAP is addressed to decision-makers of the region of Dresden (defined, for instance, as planning region). Its function is to complement and coordinate existing instruments and measures. These instruments also include instruments of environmental and spatial planning on the regional level. Spatial and environmental planning can rely on a wide range of formal and informal instruments on different spatial, administrative, and sectoral levels, e.g. land use and landscape plans. Our contribution to the EGU conference aims to clear the role and relevance of the existing formal and informal planning instruments in the region of Dresden for the process of developing the IRCAP. Firstly, a survey is conducted for the purpose of identifying all relevant planning instruments. The identification process is based on specific criteria, for example: reference to the region, contents relating to the topic of climate change respectively climate adaptation. Secondly, the presentation argues for a selection of those planning instruments which seem to be most relevant for the process of developing an IRCAP. This selection process is based on specific criteria which include, for instance, complexity of expected effects, reference to regional and sectoral vulnerability, opportunity for future change of the existing planning instruments (e.g., current process of updating), interests of project partners and stakeholders. Thirdly, as a result, an overview of relevant planning instruments in the region of Dresden is shown, including their current status and statements about their relevance for the topic of climate adaptation strategies. Finally it is derived that this procedure provides a basis for the following possibilities: Adapting existing planning instruments, integrate contents of existing planning instruments in the IRCAP process, or develop and define new strategies or measures on the way to an IRCAP.

Enhanced CDC of B cell chronic lymphocytic leukemia cells mediated by rituximab combined with a novel anti-complement factor H antibody.

PubMed

Winkler, Mark T; Bushey, Ryan T; Gottlin, Elizabeth B; Campa, Michael J; Guadalupe, Eross S; Volkheimer, Alicia D; Weinberg, J Brice; Patz, Edward F

2017-01-01

Rituximab therapy for B cell chronic lymphocytic leukemia (B-CLL) has met with mixed success. Among several factors to which resistance can be attributed is failure to activate complement dependent cytotoxicity (CDC) due to protective complement regulatory proteins, including the soluble regulator complement factor H (CFH). We hypothesized that rituximab killing of non-responsive B-CLL cells could be augmented by a novel human monoclonal antibody against CFH. The B cells from 11 patients with B-CLL were tested ex vivo in CDC assays with combinations of CFH monoclonal antibody, rituximab, and a negative control antibody. CDC of rituximab non-responsive malignant B cells from CLL patients could in some cases be augmented by the CFH monoclonal antibody. Antibody-mediated cytotoxicity of cells was dependent upon functional complement. In one case where B-CLL cells were refractory to CDC by the combination of rituximab plus CFH monoclonal antibody, additionally neutralizing the membrane complement regulatory protein CD59 allowed CDC to occur. Inhibiting CDC regulatory proteins such as CFH holds promise for overcoming resistance to rituximab therapy in B-CLL.

Defining the Complement Biomarker Profile of C3 Glomerulopathy

PubMed Central

Zhang, Yuzhou; Nester, Carla M.; Martin, Bertha; Skjoedt, Mikkel-Ole; Meyer, Nicole C.; Shao, Dingwu; Borsa, Nicolò; Palarasah, Yaseelan

2014-01-01

Background and objectives C3 glomerulopathy (C3G) applies to a group of renal diseases defined by a specific renal biopsy finding: a dominant pattern of C3 fragment deposition on immunofluorescence. The primary pathogenic mechanism involves abnormal control of the alternative complement pathway, although a full description of the disease spectrum remains to be determined. This study sought to validate and define the association of complement dysregulation with C3G and to determine whether specific complement pathway abnormalities could inform disease definition. Design, setting, participants, & measurements This study included 34 patients with C3G (17 with C3 glomerulonephritis [C3GN] and 17 with dense deposit disease [DDD]) diagnosed between 2008 and 2013 selected from the C3G Registry. Control samples (n=100) were recruited from regional blood drives. Nineteen complement biomarkers were assayed on all samples. Results were compared between C3G disease categories and with normal controls. Results Assessment of the alternative complement pathway showed that compared with controls, patients with C3G had lower levels of serum C3 (P<0.001 for both DDD and C3GN) and factor B (P<0.001 for both DDD and C3GN) as well as higher levels of complement breakdown products including C3d (P<0.001 for both DDD and C3GN) and Bb (P<0.001 for both DDD and C3GN). A comparison of terminal complement pathway proteins showed that although C5 levels were significantly suppressed (P<0.001 for both DDD and C3GN) its breakdown product C5a was significantly higher only in patients with C3GN (P<0.05). Of the other terminal pathway components (C6–C9), the only significant difference was in C7 levels between patients with C3GN and controls (P<0.01). Soluble C5b-9 was elevated in both diseases but only the difference between patients with C3GN and controls reached statistical significance (P<0.001). Levels of C3 nephritic factor activity were qualitatively higher in patients with DDD compared with patients with C3GN. Conclusions Complement biomarkers are significantly abnormal in patients with C3G compared with controls. These data substantiate the link between complement dysregulation and C3G and identify C3G interdisease differences. PMID:25341722

Complement Activation Alters Platelet Function

DTIC Science & Technology

2015-12-01

haemostatic and coagulation properties of platelets. 15. SUBJECT TERMS Platelets, Complement, Trauma, Tissue Damage 16. SECURITY CLASSIFICATION... coagulation , there is mounting evidence that they may also be important in the development and progression of inflammatory processes (Coppinger et al...receptor-ligand interactions and/or through exposure to cytokines including IL-6, other acute-phase reactants, and pro- coagulant factors such as thrombin

Aspects of the Acquisition of Object Control and ECM-Type Verbs in European Portuguese

ERIC Educational Resources Information Center

Santos, Ana Lúcia; Gonçalves, Anabela; Hyams, Nina

2016-01-01

We investigate the acquisition of sentential complementation under causative, perception, and object control verbs in European Portuguese, a language rich in complement types, including the typologically marked inflected infinitives. We tested 58 children between 3 and 5 years of age and 24 adults on a sentence completion task. The results support…

The Potassium-Argon Laser Experiment (karle): In Situ Geochronology for Planetary Missions

NASA Technical Reports Server (NTRS)

Cohen, B. A.

2016-01-01

Isotopic dating is an essential tool to establish an absolute chronology for geological events. It enables a planet's crystallization history, magmatic evolution, and alteration to be placed into the framework of solar system history. The capability for in situ geochronology will open up the ability for this crucial measurement to be accomplished as part of lander or rover complement. An in situ geochronology package can also complement sample return missions by identifying the most interesting rocks to cache or return to Earth. Appropriate application of in situ dating will enable geochronology on more terrains than can be reached with sample-return missions to the Moon, Mars, asteroids, outer planetary satellites, and other bodies that contain rocky components. The capability of flight instruments to conduct in situ geochronology is called out in the NASA Planetary Science Decadal Survey and the NASA Technology Roadmap as needing development to serve the community's needs. Beagle 2 is the only mission launched to date with the explicit aim to perform in situ K-Ar isotopic dating [1], but it failed to communicate and was lost. The first in situ K-Ar date on Mars, using SAM and APXS measurements on the Cumberland mudstone [2], yielded an age of 4.21 +/- 0.35 Ga and validated the idea of K-Ar dating on other planets, though the Curiosity method is not purpose-built for dating and requires many assumptions that degrade its precision. To get more precise and meaningful ages, multiple groups are developing dedicated in situ dating instruments.

Anti-complement activities of human breast-milk.

PubMed

Ogundele, M O

1999-08-01

It has long been observed that the human milk possesses significant anti-inflammatory properties, while simultaneously protecting the infant against many intestinal and respiratory pathogens. There is, however, a paucity of information on the degree and extent of this anti-inflammatory activity. In the present study, the inhibitory effects of different fractions of human milk on serum complement activity were analysed. Colostrum and milk samples from healthy voluntary lactating donors at different postpartum ages were obtained and pooled normal human serum was used as source of complement in a modified CH50 assay. Inherent complement activity in human milk was also investigated by measuring the deposition of an activated C3 fragment on a serum-sensitive bacteria, and by haemolytic assays. Most whole- and defatted-milk samples consistently showed a dose-dependent inhibition of the serum complement activity. This inhibition was greater in mature milk compared to transitional milk samples. It was enhanced by inactivation of milk complement, and diminished by centrifugation of milk samples, which partly removed fat and larger protein components including casein micelles. Inherent complement activity in human milk was also demonstrated by haemolysis of sensitised sheep erythrocytes and deposition of C3 fragments on solid-phase bacteria. These activities were highest in the colostrum and gradually decreased as lactation proceeded. Several natural components abundant in the fluid phase of the human breast-milk have been shown to be inhibitors of complement activation in vitro. Their physiological significance probably reside in their ability to prevent inflammatory-induced tissue damage of the delicate immature gastrointestinal tract of the new-born as well as the mammary gland itself, which may arise from ongoing complement activation.

The crucial role of the private sector.

PubMed

Barberis, M; Paxman, J M

1986-12-01

Private support for the development of family planning programs continues to grow and now includes industries that provide family planning services, commercial outlets that distribute contraceptives, community groups that help to build demand, private medical practitioners who include contraception as a part of health care, organizations that provide technical and financial assistance to developing country programs, pharmaceutical firms, and foundations that underwrite contraceptive research. Although the mix of private and public programs differs from country to country, these 2 family planning programs complement each other and often work in close partnership. The private sector has the advantages of being able to pioneer innovative programs the public sector is unwilling or unable to pursue, to bring foreign financial and technical assistance to developing countries without political implications, and to achieve financially self-sustaining family planning efforts that are linked to other development efforts. In many countries, the private sector has been instrumental in developing a national family planning program and in eliminating barriers to family planning in countries with restrictive laws and policies. The private sector has been especially important in pioneering grassroots programs that improve the status of women through education, health care, training, and economic opportunity.

Earth observing system. Output data products and input requirements, version 2.0. Volume 3: Algorithm summary tables and non-EOS data products

NASA Technical Reports Server (NTRS)

Lu, Yun-Chi; Chang, Hyo Duck; Krupp, Brian; Kumar, Ravindar; Swaroop, Anand

1992-01-01

Volume 3 assists Earth Observing System (EOS) investigators in locating required non-EOS data products by identifying their non-EOS input requirements and providing the information on data sets available at various Distributed Active Archive Centers (DAAC's), including those from Pathfinder Activities and Earth Probes. Volume 3 is intended to complement, not to duplicate, the the EOSDIS Science Data Plan (SDP) by providing detailed data set information which was not presented in the SDP. Section 9 of this volume discusses the algorithm summary tables containing information on retrieval algorithms, expected outputs and required input data. Section 10 describes the non-EOS input requirements of instrument teams and IDS investigators. Also described are the current and future data holdings of the original seven DAACS and data products planned from the future missions and projects including Earth Probes and Pathfinder Activities. Section 11 describes source of information used in compiling data set information presented in this volume. A list of data set attributes used to describe various data sets is presented in section 12 along with their descriptions. Finally, Section 13 presents the SPSO's future plan to improve this report .

A comprehensive strategy for the subtyping of patients with Fanconi anaemia: conclusions from the Spanish Fanconi Anemia Research Network.

PubMed

Antonio Casado, José; Callén, Elsa; Jacome, Ariana; Río, Paula; Castella, Maria; Lobitz, Stephan; Ferro, Teresa; Muñoz, Arturo; Sevilla, Julián; Cantalejo, Angeles; Cela, Elena; Cervera, José; Sánchez-Calero, Jesús; Badell, Isabel; Estella, Jesús; Dasí, Angeles; Olivé, Teresa; José Ortega, Juan; Rodriguez-Villa, Antonia; Tapia, María; Molinés, Antonio; Madero, Luis; Segovia, José C; Neveling, Kornelia; Kalb, Reinhard; Schindler, Detlev; Hanenberg, Helmut; Surrallés, Jordi; Bueren, Juan A

2007-04-01

Fanconi anaemia is a heterogeneous genetic disease, where 12 complementation groups have been already described. Identifying the complementation group in patients with Fanconi anaemia constitutes a direct procedure to confirm the diagnosis of the disease and is required for the recruitment of these patients in gene therapy trials. To determine the subtype of Fanconi anaemia patients in Spain, a Mediterranean country with a relatively high population (23%) of Fanconi anaemia patients belonging to the gypsy race. Most patients could be subtyped by retroviral complementation approaches in peripheral blood T cells, although some mosaic patients were subtyped in cultured skin fibroblasts. Other approaches, mainly based on western blot analysis and generation of nuclear RAD51 and FANCJ foci, were required for the subtyping of a minor number of patients. From a total of 125 patients included in the Registry of Fanconi Anaemia, samples from 102 patients were available for subtyping analyses. In 89 cases the subtype could be determined and in 8 cases exclusions of common complementation groups were made. Compared with other international studies, a skewed distribution of complementation groups was observed in Spain, where 80% of the families belonged to the Fanconi anaemia group A (FA-A) complementation group. The high proportion of gypsy patients, all of them FA-A, and the absence of patients with FA-C account for this characteristic distribution of complementation groups.

An Adaptation of the Original Fresno Test to Measure Evidence-Based Practice Competence in Pediatric Bedside Nurses.

PubMed

Laibhen-Parkes, Natasha; Kimble, Laura P; Melnyk, Bernadette Mazurek; Sudia, Tanya; Codone, Susan

2018-06-01

Instruments used to assess evidence-based practice (EBP) competence in nurses have been subjective, unreliable, or invalid. The Fresno test was identified as the only instrument to measure all the steps of EBP with supportive reliability and validity data. However, the items and psychometric properties of the original Fresno test are only relevant to measure EBP with medical residents. Therefore, the purpose of this paper is to describe the development of the adapted Fresno test for pediatric nurses, and provide preliminary validity and reliability data for its use with Bachelor of Science in Nursing-prepared pediatric bedside nurses. General adaptations were made to the original instrument's case studies, item content, wording, and format to meet the needs of a pediatric nursing sample. The scoring rubric was also modified to complement changes made to the instrument. Content and face validity, and intrarater reliability of the adapted Fresno test were assessed during a mixed-methods pilot study conducted from October to December 2013 with 29 Bachelor of Science in Nursing-prepared pediatric nurses. Validity data provided evidence for good content and face validity. Intrarater reliability estimates were high. The adapted Fresno test presented here appears to be a valid and reliable assessment of EBP competence in Bachelor of Science in Nursing-prepared pediatric nurses. However, further testing of this instrument is warranted using a larger sample of pediatric nurses in diverse settings. This instrument can be a starting point for evaluating the impact of EBP competence on patient outcomes. © 2018 Sigma Theta Tau International.

Students' Socioscientific Reasoning and Decision-making on Energy-related Issues—Development of a measurement instrument

NASA Astrophysics Data System (ADS)

Sakschewski, Mark; Eggert, Sabina; Schneider, Susanne; Bögeholz, Susanne

2014-09-01

The concept of energy is one key component of science education curricula worldwide. While it is still being taught in many science classrooms from a mainly conceptual knowledge perspective, the need to frame the concept of energy as a socioscientific issue and implement it in the context of citizenship education and education for sustainable development, is getting more and more explicit. As we will be faced with limited fossil fuels and the consequences of global climate change in the future, students have to be supported in becoming literate citizens who are able to reach informed energy-related decisions. In this article, we focus on students' reasoning and decision-making processes about socioscientific energy-related issues. In more detail, we developed a paper-and-pencil measurement instrument to assess secondary school students' competencies in this domain. The functioning of the measurement instrument was analysed with a sample of 850 students from grades 6, 8, 10 and 12 using item response theory. Findings show that the measurement instrument functions in terms of reliability and validity. Concerning student ability, elaborate reasoning and decision-making was characterised by the use of trade-offs and the ability to weigh arguments and to reflect on the structure of reasoning and decision-making processes. The developed measurement instrument provides a complement for existing test instruments on conceptual knowledge about the concept of energy. It aims to contribute to a change in teaching about energy, especially in physics education in the sense of education for sustainable development.

An Induced Pluripotent Stem Cell Patient Specific Model of Complement Factor H (Y402H) Polymorphism Displays Characteristic Features of Age-Related Macular Degeneration and Indicates a Beneficial Role for UV Light Exposure.

PubMed

Hallam, Dean; Collin, Joseph; Bojic, Sanja; Chichagova, Valeria; Buskin, Adriana; Xu, Yaobo; Lafage, Lucia; Otten, Elsje G; Anyfantis, George; Mellough, Carla; Przyborski, Stefan; Alharthi, Sameer; Korolchuk, Viktor; Lotery, Andrew; Saretzki, Gabriele; McKibbin, Martin; Armstrong, Lyle; Steel, David; Kavanagh, David; Lako, Majlinda

2017-11-01

Age-related macular degeneration (AMD) is the most common cause of blindness, accounting for 8.7% of all blindness globally. Vision loss is caused ultimately by apoptosis of the retinal pigment epithelium (RPE) and overlying photoreceptors. Treatments are evolving for the wet form of the disease; however, these do not exist for the dry form. Complement factor H polymorphism in exon 9 (Y402H) has shown a strong association with susceptibility to AMD resulting in complement activation, recruitment of phagocytes, RPE damage, and visual decline. We have derived and characterized induced pluripotent stem cell (iPSC) lines from two subjects without AMD and low-risk genotype and two patients with advanced AMD and high-risk genotype and generated RPE cells that show local secretion of several proteins involved in the complement pathway including factor H, factor I, and factor H-like protein 1. The iPSC RPE cells derived from high-risk patients mimic several key features of AMD including increased inflammation and cellular stress, accumulation of lipid droplets, impaired autophagy, and deposition of "drüsen"-like deposits. The low- and high-risk RPE cells respond differently to intermittent exposure to UV light, which leads to an improvement in cellular and functional phenotype only in the high-risk AMD-RPE cells. Taken together, our data indicate that the patient specific iPSC model provides a robust platform for understanding the role of complement activation in AMD, evaluating new therapies based on complement modulation and drug testing. Stem Cells 2017;35:2305-2320. © 2017 The Authors Stem Cells published by Wiley Periodicals, Inc. on behalf of AlphaMed Press.

Coupling complement regulators to immunoglobulin domains generates effective anti-complement reagents with extended half-life in vivo

PubMed Central

HARRIS, C L; WILLIAMS, A S; LINTON, S M; MORGAN, B P

2002-01-01

Complement activation and subsequent generation of inflammatory molecules and membrane attack complex contributes to the pathology of a number of inflammatory and degenerative diseases, including arthritis, glomerulonephritis and demyelination. Agents that specifically inhibit complement activation might prove beneficial in the treatment of these diseases. Soluble recombinant forms of the naturally occurring membrane complement regulatory proteins (CRP) have been exploited for this purpose. We have undertaken to design better therapeutics based on CRP. Here we describe the generation of soluble, recombinant CRP comprising rat decay accelerating factor (DAF) or rat CD59 expressed as Fc fusion proteins, antibody-like molecules comprising two CRP moieties in place of the antibody Fab arms (CRP-Ig). Reagents bearing DAF on each arm (DAF-Ig), CD59 on each arm (CD59-Ig) and a hybrid reagent containing both DAF and CD59 were generated. All three reagents inhibited C activation in vitro. Compared with soluble CRP lacking Fc domains, activity was reduced, but was fully restored by enzymatic release of the regulator from the Ig moiety, implicating steric constraints in reducing functional activity. In vivo studies showed that DAF-Ig, when compared to soluble DAF, had a much extended half-life in the circulation in rats and concomitantly caused a sustained reduction in plasma complement activity. When given intra-articularly to rats in a model of arthritis, DAF-Ig significantly reduced severity of disease. The data demonstrate the potential of CRP-Ig as reagents for sustained therapy of inflammatory disorders, including arthritis, but emphasize the need for careful design of fusion proteins to retain function. PMID:12165074

Complement inhibition decreases early fibrogenic events in the lung of septic baboons.

PubMed

Silasi-Mansat, Robert; Zhu, Hua; Georgescu, Constantin; Popescu, Narcis; Keshari, Ravi S; Peer, Glenn; Lupu, Cristina; Taylor, Fletcher B; Pereira, Heloise Anne; Kinasewitz, Gary; Lambris, John D; Lupu, Florea

2015-11-01

Acute respiratory distress syndrome (ARDS) induced by severe sepsis can trigger persistent inflammation and fibrosis. We have shown that experimental sepsis in baboons recapitulates ARDS progression in humans, including chronic inflammation and long-lasting fibrosis in the lung. Complement activation products may contribute to the fibroproliferative response, suggesting that complement inhibitors are potential therapeutic agents. We have been suggested that treatment of septic baboons with compstatin, a C3 convertase inhibitor protects against ARDS-induced fibroproliferation. Baboons challenged with 10(9) cfu/kg (LD50) live E. coli by intravenous infusion were treated or not with compstatin at the time of challenge or 5 hrs thereafter. Changes in the fibroproliferative response at 24 hrs post-challenge were analysed at both transcript and protein levels. Gene expression analysis showed that sepsis induced fibrotic responses in the lung as early as 24 hrs post-bacterial challenge. Immunochemical and biochemical analysis revealed enhanced collagen synthesis, induction of profibrotic factors and increased cell recruitment and proliferation. Specific inhibition of complement with compstatin down-regulated sepsis-induced fibrosis genes, including transforming growth factor-beta (TGF-β), connective tissue growth factor (CTGF), tissue inhibitor of metalloproteinase 1 (TIMP1), various collagens and chemokines responsible for fibrocyte recruitment (e.g. chemokine (C-C motif) ligand 2 (CCL2) and 12 (CCL12)). Compstatin decreased the accumulation of myofibroblasts and proliferating cells, reduced the production of fibrosis mediators (TGF-β, phospho-Smad-2 and CTGF) and inhibited collagen deposition. Our data demonstrate that complement inhibition effectively attenuates collagen deposition and fibrotic responses in the lung after severe sepsis. Inhibiting complement could prove an attractive strategy for preventing sepsis-induced fibrosis of the lung. © 2015 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

The OCO-3 Mission: Science Objectives and Instrument Performance

NASA Astrophysics Data System (ADS)

Eldering, A.; Basilio, R. R.; Bennett, M. W.

2017-12-01

The Orbiting Carbon Observatory 3 (OCO-3) will continue global CO2 and solar-induced chlorophyll fluorescence (SIF) using the flight spare instrument from OCO-2. The instrument is currently being tested, and will be packaged for installation on the International Space Station (ISS) (launch readiness in early 2018.) This talk will focus on the science objectives, updated simulations of the science data products, and the outcome of recent instrument performance tests. The low-inclination ISS orbit lets OCO-3 sample the tropics and sub-tropics across the full range of daylight hours with dense observations at northern and southern mid-latitudes (+/- 52º). The combination of these dense CO2 and SIF measurements provides continuity of data for global flux estimates as well as a unique opportunity to address key deficiencies in our understanding of the global carbon cycle. The instrument utilizes an agile, 2-axis pointing mechanism (PMA), providing the capability to look towards the bright reflection from the ocean and validation targets. The PMA also allows for a snapshot mapping mode to collect dense datasets over 100km by 100km areas. Measurements over urban centers could aid in making estimates of fossil fuel CO2 emissions. Similarly, the snapshot mapping mode can be used to sample regions of interest for the terrestrial carbon cycle. In addition, there is potential to utilize data from ISS instruments ECOSTRESS (ECOsystem Spaceborne Thermal Radiometer Experiment on Space Station) and GEDI (Global Ecosystem Dynamics Investigation), which measure other key variables of the control of carbon uptake by plants, to complement OCO-3 data in science analysis. In 2017, the OCO-2 instrument was transformed into the ISS-ready OCO-3 payload. The transformed instrument was thoroughly tested and characterized. Key characteristics, such as instrument ILS, spectral resolution, and radiometric performance will be described. Analysis of direct sun measurements taken during testing will also be discussed.

Aeronomy of the Venus Upper Atmosphere

NASA Astrophysics Data System (ADS)

Gérard, J.-C.; Bougher, S. W.; López-Valverde, M. A.; Pätzold, M.; Drossart, P.; Piccioni, G.

2017-11-01

We present aeronomical observations collected using remote sensing instruments on board Venus Express, complemented with ground-based observations and numerical modeling. They are mostly based on VIRTIS and SPICAV measurements of airglow obtained in the nadir mode and at the limb above 90 km. They complement our understanding of the behavior of Venus' upper atmosphere that was largely based on Pioneer Venus observations mostly performed over thirty years earlier. Following a summary of recent spectral data from the EUV to the infrared, we examine how these observations have improved our knowledge of the composition, thermal structure, dynamics and transport of the Venus upper atmosphere. We then synthesize progress in three-dimensional modeling of the upper atmosphere which is largely based on global mapping and observations of time variations of the nitric oxide and O2 nightglow emissions. Processes controlling the escape flux of atoms to space are described. Results based on the VeRA radio propagation experiment are summarized and compared to ionospheric measurements collected during earlier space missions. Finally, we point out some unsolved and open questions generated by these recent datasets and model comparisons.

Complementing the Australian Primary School Health and Physical Education (HPE) Curriculum: Exploring Children's HPE Learning Experiences within Varying School Ground Equipment Contexts

ERIC Educational Resources Information Center

Hyndman, Brendon; Mahony, Linda; Te Ava, Aue; Smith, Sue; Nutton, Georgie

2017-01-01

This paper unearths how primary school children experience and can complement the Australian HPE curriculum within three unique school ground equipment scenarios that include an "empty", "loose parts" and a "traditional" school ground context. Using direct observation, 490 scans were undertaken of the school grounds…

The Far Ultraviolet (FUV) auroral imager for the Inner Magnetospheric Imager (IMI) mission: Options

NASA Technical Reports Server (NTRS)

Wilson, Gordon R.

1993-01-01

The change from an intermediate mission (cost ceiling of $300 million) to a solar-terrestrial probe class mission (cost ceiling of $150 million) will require some major changes in the configuration of the IMI mission. One option being considered is to move to a small spin-stabilized spacecraft (with no despun platform) which could be launched with a smaller Taurus or Conestoga class booster. Such a change in spacecraft type would not present any fundamental problems (other than restrictions on mass and power) for the He plus 304 A plasmasphere imager, the high and low energy neutral atom imagers, and the geocoronal imager, but would present a challenge for the FUV auroral imager since the original plan called for this instrument to operate from a despun platform. Since the FUV instrument is part of the core payload it cannot be dropped from the instrument complement without jeopardizing the science goals of the mission. A way must be found to keep this instrument and to allow it to accomplish most, if not all, of its science objectives. One of the subjects discussed are options for building an FUV instrument for a spinning spacecraft. Since a number of spinning spacecraft have carried auroral imagers, a range of techniques exists. In addition, the option of flying the FUV imager on a separate micro-satellite launched with the main IMI spacecraft or with a separate pegasus launch, was considered and is discussed.

Instrument-mounted displays for reducing cognitive load during surgical navigation.

PubMed

Herrlich, Marc; Tavakol, Parnian; Black, David; Wenig, Dirk; Rieder, Christian; Malaka, Rainer; Kikinis, Ron

2017-09-01

Surgical navigation systems rely on a monitor placed in the operating room to relay information. Optimal monitor placement can be challenging in crowded rooms, and it is often not possible to place the monitor directly beside the situs. The operator must split attention between the navigation system and the situs. We present an approach for needle-based interventions to provide navigational feedback directly on the instrument and close to the situs by mounting a small display onto the needle. By mounting a small and lightweight smartwatch display directly onto the instrument, we are able to provide navigational guidance close to the situs and directly in the operator's field of view, thereby reducing the need to switch the focus of view between the situs and the navigation system. We devise a specific variant of the established crosshair metaphor suitable for the very limited screen space. We conduct an empirical user study comparing our approach to using a monitor and a combination of both. Results from the empirical user study show significant benefits for cognitive load, user preference, and general usability for the instrument-mounted display, while achieving the same level of performance in terms of time and accuracy compared to using a monitor. We successfully demonstrate the feasibility of our approach and potential benefits. With ongoing technological advancements, instrument-mounted displays might complement standard monitor setups for surgical navigation in order to lower cognitive demands and for improved usability of such systems.

A distributed atmosphere-sea ice-ocean observatory in the central Arctic Ocean: concept and first results

NASA Astrophysics Data System (ADS)

Hoppmann, Mario; Nicolaus, Marcel; Rabe, Benjamin; Wenzhöfer, Frank; Katlein, Christian; Scholz, Daniel; Valcic, Lovro

2017-04-01

To understand the current evolution of the Arctic Ocean towards a less extensive, thinner and younger sea ice cover is one of the biggest challenges in climate research. Especially the lack of simultaneous in-situ observations of sea ice, ocean and atmospheric properties leads to significant knowledge gaps in their complex interactions, and how the associated processes impact the polar marine ecosystem. Here we present a concept for the implementation of a long-term strategy to monitor the most essential climate- and ecosystem parameters in the central Arctic Ocean, year round and synchronously. The basis of this strategy is the development and enhancement of a number of innovative autonomous observational platforms, such as rugged weather stations, ice mass balance buoys, ice-tethered bio-optical buoys and upper ocean profilers. The deployment of those complementing platforms in a distributed network enables the simultaneous collection of physical and biogeochemical in-situ data on basin scales and year round, including the largely undersampled winter periods. A key advantage over other observatory systems is that the data is sent via satellite in near-real time, contributing to numerical weather predictions through the Global Telecommunication System (GTS) and to the International Arctic Buoy Programme (IABP). The first instruments were installed on ice floes in the Eurasian Basin in spring 2015 and 2016, yielding exceptional records of essential climate- and ecosystem-relevant parameters in one of the most inaccessible regions of this planet. Over the next 4 years, and including the observational periods of the Year of Polar Prediction (YOPP, 2017-2019) and the Multidisciplinary drifting Observatory for the Study of the Arctic Climate (MOSAiC, 2020), the distributed observatory will be maintained by deployment of additional instruments in the central Arctic each year, benefitting from international logistical efforts.

[Health promotion effectiveness: developing and testing a system for routine evaluation in health education, workplace health promotion and setting approach supplied by the German statutory health insurance agencies].

PubMed

Kliche, T; Riemann, K; Bockermann, C; Niederbühl, K; Wanek, V; Koch, U

2011-04-01

The aim of the study was to develop and test a routine evaluation system for all health promotion and education activities funded by the German statutory health insurance companies. The system aims at measuring both individual health effects and the complex organisational effects of setting projects. Measurement instruments were developed synoptically and tested in three field tests (2003-2008). In order to assess the impact of individual health training, 212 courses of the health insurance companies were evaluated. To assess the setting approach, 56 schools participating in a health-promotion project were included, and for workplace health-promotion 6 projects of different health insurance companies were included. The research design was an observational study. Instead of control groups, individual data were compared to reference values for gender- and age-matched groups from national health surveys. The studies consisted of baseline and final assessment (T1/T2), complemented by a follow-up (T3), all adapted to the time of intervention (i. e., 3-24 months for T1/T2 and 3-18 months for T2/T3). The evaluation system provides multilevel-measurement based upon validated questionnaires for health-related structures and processes in institutions, and for the participating individual's subjective health, health problems, health-related quality of life, workplace and institutional satisfaction. Controlling for central confounders is also possible (input and dosage, age, gender, educational background). Thus, short but valid measurement instruments of high usability are available to evaluate the effectiveness of prevention, health promotion and education. © Georg Thieme Verlag KG Stuttgart · New York.

[Spleen autotransplant. Natural history and description of a case].

PubMed

Ceccherini, E; Sereni, P; Ferrari, F; Fagioli Zucchi, A; Croce, F; Di Maggio, G; Vattimo, A; Mancini, S

1989-09-30

After considering the natural history of spleen auto-transplant, a clinical case followed up for seven months with instrumental (echography, scintigraphy) and humoral (Jolly bodies, Heinz bodies, reticulocytes, platelets, complement, immune globulin) examinations has been considered so as to verify "take" and function. One months after reimplantation the patient was again operated on for the onset of an intestinal occlusion due to adherences. On that occasion it was possible to control that the implant had taken. It is concluded that personally used parameters proved to be well correlated and that scintigraphy and echography are two complementary, effective techniques for monitoring auto-transplants.

About Jupiter's Reflectance Function in JunoCam Images

NASA Astrophysics Data System (ADS)

Eichstaedt, G.; Orton, G. S.; Momary, T.; Hansen, C. J.; Caplinger, M.

2017-09-01

NASA's Juno spacecraft has successfully completed several perijove passes. JunoCam is Juno's visible light and infrared camera. It was added to the instrument complement to investigate Jupiter's polar regions, and for education and public outreach purposes. Images of Jupiter taken by JunoCam have been revealing effects that can be interpreted as caused by a haze layer. This presumed haze layer appears to be structured, and it partially obscures Jupiter's cloud top. With empirical investigation of Jupiter's reflectance function we intend to separate light contributed by haze from light reflected off Jupiter's cloud tops, enabling both layers to be investigated separately.

Paper-based analytical devices for environmental analysis.

PubMed

Meredith, Nathan A; Quinn, Casey; Cate, David M; Reilly, Thomas H; Volckens, John; Henry, Charles S

2016-03-21

The field of paper-based microfluidics has experienced rapid growth over the past decade. Microfluidic paper-based analytical devices (μPADs), originally developed for point-of-care medical diagnostics in resource-limited settings, are now being applied in new areas, such as environmental analyses. Low-cost paper sensors show great promise for on-site environmental analysis; the theme of ongoing research complements existing instrumental techniques by providing high spatial and temporal resolution for environmental monitoring. This review highlights recent applications of μPADs for environmental analysis along with technical advances that may enable μPADs to be more widely implemented in field testing.

STS-35 MS Hoffman operates ASTRO-1 MPC on OV-102's aft flight deck

NASA Image and Video Library

1990-12-10

STS035-12-015 (2-11 Dec 1990) --- Astronaut Jeffrey A. Hoffman, STS 35 mission specialist, uses a manual pointing controller (MPC) for the Astro-1 mission's Instrument Pointing System (IPS). By using the MPC, Hoffman and other crewmembers on Columbia's aft flight deck, were able to command the IPS, located in the cargo bay, to record astronomical data. Hoffman is serving the "Blue" shift which complemented the currently sleeping "Red" shift of crewmembers as the mission collected scientific data on a 24-hour basis. The scene was photographed with a 35mm camera.

OV-104's RMS releases Gamma Ray Observatory (GRO) during STS-37 deployment

NASA Image and Video Library

1991-04-07

Atlantis', Orbiter Vehicle (OV) 104's, remote manipulator system (RMS) releases Gamma Ray Observatory (GRO) during STS-37 deployment. Visible on the GRO as it drifts away from the RMS end effector are the four complement instruments: the Energetic Gamma Ray Experiment (bottom); Imaging Compton Telescope (COMPTEL) (center); Oriented Scintillation Spectrometer Experiment (OSSE) (top); and Burst and Transient Source Experiment (BATSE) (at four corners). GRO's solar array (SA) panels are extended and are in orbit configuration. View was taken through aft flight deck window which reflects some of the crew compartment interior.

European Space Agency /ESA/ pointing systems - Requirements and fulfillment

NASA Astrophysics Data System (ADS)

Nellessen, W.; Wolf, P.

1982-02-01

The Instrument Pointing System (IPS), a three-axes gimballed experimentation facility, is to be first used in the second Spacelab flight, in 1984. In response to crew motions and Space Shuttle thruster disturbances, payload mass and inertia, and IPS positioning in the cargo bay, the IPS will provide a stable pointing capability for payloads of up to 2000 kg whose accuracy is in the 1-10 arcsec range. The IPS is complemented in the up-to-200 kg payload mass range by a small Position and Hold Mount, a functional model of which is now available for performance demonstration tests.

A complementation assay for in vivo protein structure/function analysis in Physcomitrella patens (Funariaceae)

DOE PAGES

Scavuzzo-Duggan, Tess R.; Chaves, Arielle M.; Roberts, Alison W.

2015-07-14

Here, a method for rapid in vivo functional analysis of engineered proteins was developed using Physcomitrella patens. A complementation assay was designed for testing structure/function relationships in cellulose synthase (CESA) proteins. The components of the assay include (1) construction of test vectors that drive expression of epitope-tagged PpCESA5 carrying engineered mutations, (2) transformation of a ppcesa5 knockout line that fails to produce gametophores with test and control vectors, (3) scoring the stable transformants for gametophore production, (4) statistical analysis comparing complementation rates for test vectors to positive and negative control vectors, and (5) analysis of transgenic protein expression by Westernmore » blotting. The assay distinguished mutations that generate fully functional, nonfunctional, and partially functional proteins. In conclusion, compared with existing methods for in vivo testing of protein function, this complementation assay provides a rapid method for investigating protein structure/function relationships in plants.« less

A comprehensive strategy for the subtyping of patients with Fanconi anaemia: conclusions from the Spanish Fanconi Anemia Research Network

PubMed Central

Casado, José Antonio; Callén, Elsa; Jacome, Ariana; Río, Paula; Castella, Maria; Lobitz, Stephan; Ferro, Teresa; Muñoz, Arturo; Sevilla, Julián; Cantalejo, Ángeles; Cela, Elena; Cervera, José; Sánchez‐Calero, Jesús; Badell, Isabel; Estella, Jesús; Dasí, Ángeles; Olivé, Teresa; Ortega, Juan José; Rodriguez‐Villa, Antonia; Tapia, María; Molinés, Antonio; Madero, Luis; Segovia, José C; Neveling, Kornelia; Kalb, Reinhard; Schindler, Detlev; Hanenberg, Helmut; Surrallés, Jordi; Bueren, Juan A

2007-01-01

Background Fanconi anaemia is a heterogeneous genetic disease, where 12 complementation groups have been already described. Identifying the complementation group in patients with Fanconi anaemia constitutes a direct procedure to confirm the diagnosis of the disease and is required for the recruitment of these patients in gene therapy trials. Objective To determine the subtype of Fanconi anaemia patients in Spain, a Mediterranean country with a relatively high population (23%) of Fanconi anaemia patients belonging to the gypsy race. Methods Most patients could be subtyped by retroviral complementation approaches in peripheral blood T cells, although some mosaic patients were subtyped in cultured skin fibroblasts. Other approaches, mainly based on western blot analysis and generation of nuclear RAD51 and FANCJ foci, were required for the subtyping of a minor number of patients. Results and conclusions From a total of 125 patients included in the Registry of Fanconi Anaemia, samples from 102 patients were available for subtyping analyses. In 89 cases the subtype could be determined and in 8 cases exclusions of common complementation groups were made. Compared with other international studies, a skewed distribution of complementation groups was observed in Spain, where 80% of the families belonged to the Fanconi anaemia group A (FA‐A) complementation group. The high proportion of gypsy patients, all of them FA‐A, and the absence of patients with FA‐C account for this characteristic distribution of complementation groups. PMID:17105750

BepiColombo: Exploring Mercury

NASA Astrophysics Data System (ADS)

Geelen, K.; Novara, M.; Fugger, S.; Benkhoff, J.

2014-04-01

BepiColombo is an interdisciplinary mission to explore Mercury, the planet closest to the sun, carried out jointly between the European Space Agency and the Japanese Aerospace Exploration Agency. The mission consists of two orbiters dedicated to the detailed study of the planet and of its magnetosphere, the Mercury Planetary Orbiter (MPO) and the Mercury Magnetospheric Orbiter (MMO). The MPO is ESA's scientific contribution to the mission and comprises 11 science instruments. It is a three-axis-stabilized, nadir-pointing spacecraft which will be placed in a polar orbit with a period of approximately 2.3 hours, a periapsis of 480 km and an apoapsis of 1500 km, providing excellent spatial resolution over the entire planet surface. The interplanetary transfer is performed by an Electric Propulsion Module, which is jettisoned when Mercury is reached. It will set off in July 2016 on a journey to the smallest and least explored terrestrial planet in our Solar System. When it arrives at Mercury in January 2024, it will endure temperatures in excess of 350 °C and gather data during its 1 year nominal mission, with a possible 1-year extension. The difficulty of reaching, surviving and operating in the harsh environment of a planet so close to the sun, makes BepiColombo one of the most challenging planetary projects undertaken by ESA so far. A range of major challenges need to be overcome to enable the mission including the electric propulsion system, development of a new Multi-Layer Insulation able to withstand the high temperatures, an original solar panel design, stringent pointing requirements to be maintained in extreme conditions varying from a solar flux of 10 solar constants to eclipse conditions etc. The scientific payload of both spacecraft will provide the detailed information necessary to understand the origin and evolution of the planet itself and its surrounding environment. The scientific objectives focus on a global characterization of Mercury through the investigation of its interior, surface,exosphere and magnetosphere. In addition, instrumentation onboard BepiColombo will be used to test Einstein's theory of general relativity. Major effort was put into optimizing the scientific return of the mission by defining a payload complement such that individual measurements can be interrelated and complement each other. This paper gives an in-depth overview of BepiColombo spacecraft composite and the mission profile. It describes the suite of scientific instruments on board of the two BepiColombo spacecraft and the science goals of the mission. This paper gives an overview of the mission, describes the science case together with the payload suite as well as the latest status of the spacecraft development.

Observations of Interplanetary Scintillation (IPS) Using the Mexican Array Radio Telescope (MEXART)

NASA Astrophysics Data System (ADS)

Mejia-Ambriz, J. C.; Villanueva-Hernandez, P.; Gonzalez-Esparza, J. A.; Aguilar-Rodriguez, E.; Jeyakumar, S.

2010-08-01

The Mexican Array Radio Telescope (MEXART) consists of a 64×64 (4096) full-wavelength dipole antenna array, operating at 140 MHz, with a bandwidth of 2 MHz, occupying about 9660 square meters (69 m × 140 m) ( http://www.mexart.unam.mx ). This is a dedicated radio array for Interplanetary Scintillation (IPS) observations located at latitude 19°48'N, longitude 101°41'W. We characterize the performance of the system. We report the first IPS observations with the instrument, employing a Butler Matrix (BM) of 16×16 ports, fed by 16 east - west lines of 64 dipoles (1/4 of the total array). The BM displays a radiation pattern of 16 beams at different declinations (from -48, to +88 degrees). We present a list of 19 strong IPS radio sources (having at least 3 σ in power gain) detected by the instrument. We report the power spectral analysis procedure of the intensity fluctuations. The operation of MEXART will allow us a better coverage of solar wind disturbances, complementing the data provided by the other, previously built, instruments.

Inner Magnetosphere Imager (IMI) solar terrestrial probe class mission preliminary design study report

NASA Technical Reports Server (NTRS)

Hermann, M.; Johnson, L.

1994-01-01

For three decades, magnetospheric field and plasma measurements have been made by diverse instruments flown on spacecraft in many different orbits, widely separated in space and time, and under various solar and magnetospheric conditions. Scientists have used this information to piece together an intricate, yet incomplete view of the magnetosphere. A simultaneous global view, using various light wavelengths and energetic neutral atoms, could reveal exciting new data and help explain complex magnetospheric processes, thus providing us with a clear picture of this region of space. The George C. Marshall Space Flight Center (MSFC) is responsible for defining the IMI mission which will study this region of space. NASA's Space Physics Division of the Office of Space Science placed the IMI third in its queue of Solar Terrestrial Probe missions for launch in the 1990's. A core instrument complement of three images (with the potential addition of one or more mission enhancing instruments) will fly in an elliptical, polar earth orbit with an apogee of 44,600 km and a perigee of 4,800 km. This paper will address the mission objectives, spacecraft design consideration, interim results of the MSFC concept definition study, and future plans.

Development of an Audio Microphone for the Mars Surveyor 98 Lander

NASA Astrophysics Data System (ADS)

Delory, G. T.; Luhmann, J. G.; Curtis, D. W.; Friedman, L. D.; Primbsch, J. H.; Mozer, F. S.

1998-01-01

In December 1999, the next Mars Surveyor Lander will bring the first microphone to the surface of Mars. The Mars Microphone represents a joint effort between the Planetary Society and the University of California at Berkeley Space Sciences Laboratory and is riding on the lander as part of the LIDAR instrument package provided by the Russian Academy of Sciences in Moscow. The inclusion of a microphone on the Mars Surveyor Lander represents a unique opportunity to sample for the first time the acoustic environment on the surface, including both natural and lander-generated sounds. Sounds produced by martian meteorology are among the signals to be recorded, including wind and impacts of sand particles on the instrument. Photographs from the Viking orbiters as well as Pathfinder images show evidence of small tornado-like vortices that may be acoustically detected, along with noise generated by static discharges possible during sandstorms. Lander-generated sounds that will be measured include the motion and digging of the lander arm as it gathers soil samples for analysis. Along with these scientific objectives, the Mars Microphone represents a powerful tool for public outreach by providing sound samples on the Internet recorded during the mission. The addition of audio capability to the lander brings us one step closer to a true virtual presence on the Mars surface by complementing the visual capabilities of the Mars Surveyor cameras. The Mars Microphone is contained in a 5 x 5 x 1 cm box, weighs less than 50 g, and uses 0.1 W of power during its most active times. The microphone used is a standard hearing-aid electret. The sound sampling and processing system relies on an RSC-164 speech processor chip, which performs 8-bit A/ D sampling and sound compression. An onboard flight program enables several modes for the instrument, including varying sample ranges of 5 kHz and 20 kHz, and a selectable gain setting with 64x dynamic range. The device automatically triggers on the loudest sound during a collection period for storage in an internal flash memory. Data returned by the lander consist of a compressed time-series acoustic waveform, between 2 and 10 s long, depending on the sample rate. In addition to the discrete waveform. capture, the instrument continuously records the mean power in each of six frequency bands in order to provide an average characterization of the martian acoustic environment. Once the data are retrieved from the telemetry, the compressed time series is expanded into a standard PC-compatible WAV file for analysis, which will include representation in spectral format using FFTs for quantitative characterization of the sound data. The WAV files will be used to share the data with the public via the Internet. The Mars Microphone will thus fulfill a dual role on the Mars Surveyor mission, one as a possible precursor to a more sophisticated acoustic instrument on future landers. and one as a mechanism to increase public awareness of efforts to explore and understand the martian climate and planetary history.

C1q-Mediated Complement Activation and C3 Opsonization Trigger Recognition of Stealth Poly(2-methyl-2-oxazoline)-Coated Silica Nanoparticles by Human Phagocytes.

PubMed

Tavano, Regina; Gabrielli, Luca; Lubian, Elisa; Fedeli, Chiara; Visentin, Silvia; Polverino De Laureto, Patrizia; Arrigoni, Giorgio; Geffner-Smith, Alessandra; Chen, Fangfang; Simberg, Dmitri; Morgese, Giulia; Benetti, Edmondo M; Wu, Linping; Moghimi, Seyed Moein; Mancin, Fabrizio; Papini, Emanuele

2018-05-23

Poly(2-methyl-2-oxazoline) (PMOXA) is an alternative promising polymer to poly(ethylene glycol) (PEG) for design and engineering of macrophage-evading nanoparticles (NPs). Although PMOXA-engineered NPs have shown comparable pharmacokinetics and in vivo performance to PEGylated stealth NPs in the murine model, its interaction with elements of the human innate immune system has not been studied. From a translational angle, we studied the interaction of fully characterized PMOXA-coated vinyltriethoxysilane-derived organically modified silica NPs (PMOXA-coated NPs) of approximately 100 nm in diameter with human complement system, blood leukocytes, and macrophages and compared their performance with PEGylated and uncoated NP counterparts. Through detailed immunological and proteomic profiling, we show that PMOXA-coated NPs extensively trigger complement activation in human sera exclusively through the classical pathway. Complement activation is initiated by the sensing molecule C1q, where C1q binds with high affinity ( K d = 11 ± 1 nM) to NP surfaces independent of immunoglobulin binding. C1q-mediated complement activation accelerates PMOXA opsonization with the third complement protein (C3) through the amplification loop of the alternative pathway. This promoted NP recognition by human blood leukocytes and monocyte-derived macrophages. The macrophage capture of PMOXA-coated NPs correlates with sera donor variability in complement activation and opsonization but not with other major corona proteins, including clusterin and a wide range of apolipoproteins. In contrast to these observations, PMOXA-coated NPs poorly activated the murine complement system and were marginally recognized by mouse macrophages. These studies provide important insights into compatibility of engineered NPs with elements of the human innate immune system for translational steps.

Mouse genetics and proteomic analyses demonstrate a critical role for complement in a model of DHRD/ML, an inherited macular degeneration

PubMed Central

Garland, Donita L.; Fernandez-Godino, Rosario; Kaur, Inderjeet; Speicher, Kaye D.; Harnly, James M.; Lambris, John D.; Speicher, David W.; Pierce, Eric A.

2014-01-01

Macular degenerations, inherited and age related, are important causes of vision loss. Human genetic studies have suggested perturbation of the complement system is important in the pathogenesis of age-related macular degeneration. The mechanisms underlying the involvement of the complement system are not understood, although complement and inflammation have been implicated in drusen formation. Drusen are an early clinical hallmark of inherited and age-related forms of macular degeneration. We studied one of the earliest stages of macular degeneration which precedes and leads to the formation of drusen, i.e. the formation of basal deposits. The studies were done using a mouse model of the inherited macular dystrophy Doyne Honeycomb Retinal Dystrophy/Malattia Leventinese (DHRD/ML) which is caused by a p.Arg345Trp mutation in EFEMP1. The hallmark of DHRD/ML is the formation of drusen at an early age, and gene targeted Efemp1R345W/R345W mice develop extensive basal deposits. Proteomic analyses of Bruch's membrane/choroid and Bruch's membrane in the Efemp1R345W/R345W mice indicate that the basal deposits comprise normal extracellular matrix (ECM) components present in abnormal amounts. The proteomic analyses also identified significant changes in proteins with immune-related function, including complement components, in the diseased tissue samples. Genetic ablation of the complement response via generation of Efemp1R345W/R345W:C3−/− double-mutant mice inhibited the formation of basal deposits. The results demonstrate a critical role for the complement system in basal deposit formation, and suggest that complement-mediated recognition of abnormal ECM may participate in basal deposit formation in DHRD/ML and perhaps other macular degenerations. PMID:23943789

A device for high-throughput monitoring of degradation in soft tissue samples.

PubMed

Tzeranis, D S; Panagiotopoulos, I; Gkouma, S; Kanakaris, G; Georgiou, N; Vaindirlis, N; Vasileiou, G; Neidlin, M; Gkousioudi, A; Spitas, V; Macheras, G A; Alexopoulos, L G

2018-06-06

This work describes the design and validation of a novel device, the High-Throughput Degradation Monitoring Device (HDD), for monitoring the degradation of 24 soft tissue samples over incubation periods of several days inside a cell culture incubator. The device quantifies sample degradation by monitoring its deformation induced by a static gravity load. Initial instrument design and experimental protocol development focused on quantifying cartilage degeneration. Characterization of measurement errors, caused mainly by thermal transients and by translating the instrument sensor, demonstrated that HDD can quantify sample degradation with <6 μm precision and <10 μm temperature-induced errors. HDD capabilities were evaluated in a pilot study that monitored the degradation of fresh ex vivo human cartilage samples by collagenase solutions over three days. HDD could robustly resolve the effects of collagenase concentration as small as 0.5 mg/ml. Careful sample preparation resulted in measurements that did not suffer from donor-to-donor variation (coefficient of variance <70%). Due to its unique combination of sample throughput, measurement precision, temporal sampling and experimental versality, HDD provides a novel biomechanics-based experimental platform for quantifying the effects of proteins (cytokines, growth factors, enzymes, antibodies) or small molecules on the degradation of soft tissues or tissue engineering constructs. Thereby, HDD can complement established tools and in vitro models in important applications including drug screening and biomaterial development. Copyright © 2018 Elsevier Ltd. All rights reserved.

CORSAIR-Calibrated Observations of Radiance Spectra from the Atmosphere in the Far- Infrared

NASA Astrophysics Data System (ADS)

Mlynczak, M. G.; Johnson, D.; Abedin, N.; Liu, X.; Kratz, D.; Jordan, D.; Wang, J.; Bingham, G.; Latvakoski, H.; Bowman, K.; Kaplan, S.

2008-12-01

The CORSAIR project is a new NASA Instrument Incubator Project (IIP) whose primary goal is to develop and demonstrate the necessary technologies to achieve SI-traceable, on-orbit measurements of Earth's spectral radiance in the far-infrared (far-IR). The far-IR plays a vital role in the energy balance of the Earth yet its spectrum has not been comprehensively observed from space for the purposes of climate sensing. The specific technologies being developed under CORSAIR include: passively cooled, antenna-coupled terahertz detectors for the far-IR (by Raytheon Vision Systems); accurately calibrated, SI-traceable blackbody sources for the far-IR (by Space Dynamics Laboratory); and high-performance broad bandpass beamsplitters (by ITT). These technologies complement those already developed under past Langley IIP projects (FIRST; INFLAME) in the areas of Fourier Transform Spectrometers and dedicated far-IR beamsplitters. The antenna-coupled far-IR detectors will be validated in the FIRST instrument at Langley. The SI-traceable far-IR blackbodies will be developed in conjunction with the National Institute of Standards and Technology (NIST). An overview of the CORSAIR technologies will be presented as well as their larger role in the Climate Absolute Radiance and Refractivity Observatory (CLARREO) mission. Upon successful completion of CORSAIR these IIP efforts will provide the necessary technologies to achieve the first comprehensive, accurate, high-resolution measurements from a satellite of the far-IR spectrum of the Earth and its atmosphere, enabling major advances in our understanding of Earth's climate.

Fear and/or anxiety of children and parents associated with the dental environment.

PubMed

Leal, A M; Serra, K G; Queiroz, R C; Araújo, M A; Maia Filho, E M

2013-12-01

To assess levels of anxiety in children concerning different dental instruments and equipment and to relate them with parents' anxiety levels moments before the appointment. Fifty children from 4 to 12 years of age (average of 10±3.07) and their respective parents were evaluated. A facial scale was used to assess children's anxiety levels, while the Dental Anxiety Scale (DAS) was used to assess parents. Friedman test was applied to check any differences in children's anxiety levels relative to the equipment/instruments, and this was complemented by the use of the Wilcoxon test for two-by-two comparison. In order to assess correlation between parents' and their children's anxiety levels, the study used Spearman correlation coefficient. With regard to parents' anxiety levels, 4% resulted as null, 18% were low, 56% were moderate, and 22% were exacerbated; children's anxiety level results were: 52% light, 44% intermediate, and 4% intense. Anxiety levels related to instruments/equipment were, in descending order: carpule syringe > paediatric forceps > dental explorer > x-ray machine > rubber dam punch > high speed handpiece > rubber dam forceps > mouth mirror > clinical tweezers > dental chair. No correlation was found between parents' anxiety levels and those of their children (p=0.546). The instruments/equipment used in the assessment generated different anxiety levels in the children. No correlation was found between parents' anxiety levels and those of their children.

SWICS/Ulysses and MASS/wind observations of solar wind sulfur charge states

NASA Technical Reports Server (NTRS)

Cohen, C. M. S.; Galvin, A. B.; Hamilton, D. C.; Gloeckler, G.; Geiss, J.; Bochsler, P.

1995-01-01

As Ulysses journeys from the southern to the northern solar pole, the newly launched Wind spacecraft is monitoring the solar wind near 1 AU, slightly upstream of the Earth. Different solar wind structures pass over both spacecraft as coronal holes and other features rotate in and out of view. Ulysses and Wind are presently on opposing sides of the sun allowing us to monitor these streams for extended periods of time. Composition measurements made by instruments on both spacecraft provide information concerning the evolution and properties of these structures. We have combined data from the Solar Wind Ion Composition Spectrometer (SWICS) on Ulysses and the high mass resolution spectrometer (MASS) on Wind to determine the charge state distribution of sulfur in the solar wind. Both instruments employ electrostatic deflection with time-of-flight measurement. The high mass resolution of the MASS instrument (M/Delta-M approximately 100) allows sulfur to be isolated easily while the stepping energy/charge selection provides charge state information. SWICS measurements allow the unique identification of heavy ions by their mass and mass/charge with resolutions of M/Delta-M approximately 3 and M/q/Delta(M/q) approximately 20. The two instruments complement each other nicely in that MASS has the greater mass resolution while SWICS has the better mass/charge resolution and better statistics.

A Persian-version of the stuttering severity instrument-version four (SSI-4): How the new additions to SSI-4 complement its stuttering severity score?

PubMed

Tahmasebi, Neda; Shafie, Bijan; Karimi, Hamid; Mazaheri, Masood

The fourth version of the Stuttering Severity Instrument (SSI-4) has been available since 2009. It has some modifications and new features which make it more appropriate at least for clinical practice, although further documentation is needed. The objective of the current research was to translate SSI-4 into Persian language and to discuss its relative and absolute reliability as well as its criterion validity for Persian adults who stutter (PWS). We also aimed to study how the new subjective self-reports of the SSI-4 complement the stuttering severity score obtained from the SSI-3 or the SSI-4. The cross-cultural guideline recommended by the International Quality of Life Assessment project was used to translate the SSI-4 into Persian language. Thirty five PWS from ages 17 to 42 were recruited and 10 speech and language pathologists assessed their stuttering severity using either the SSI-4 or stuttering severity ratings (SR) to test validity and reliability of the Persian translated version. A very high inter-judge relative reliability along with a poor absolute inter-judge reliability was found for the SSI-4 scores. The results were more promising for the intra-judge absolute reliability. Test-retest reliability of the complementary questions to the SSI-4 was also found acceptable. However, no strong relationship was found between the SSI-4 scores and its complementary questions. The Persian version of the SSI-4 can be used reliably by trained SLPs for research and clinical purposes, but not to document small changes in stuttering severity. We argue that the response of participants to the complementary self-report questions should also be considered in calculating their stuttering severity score. Copyright © 2018 Elsevier Inc. All rights reserved.

Plasma-based proteomics reveals immune response, complement and coagulation cascades pathway shifts in heat-stressed lactating dairy cows.

PubMed

Min, Li; Cheng, Jianbo; Zhao, Shengguo; Tian, He; Zhang, Yangdong; Li, Songli; Yang, Hongjian; Zheng, Nan; Wang, Jiaqi

2016-09-02

Heat stress (HS) has an enormous economic impact on the dairy industry. In recent years, many researchers have investigated changes in the gene expression and metabolomics profiles in dairy cows caused by HS. However, the proteomics profiles of heat-stressed dairy cows have not yet been completely elucidated. We compared plasma proteomics from HS-free and heat-stressed dairy cows using an iTRAQ labeling approach. After the depletion of high abundant proteins in the plasma, 1472 proteins were identified. Of these, 85 proteins were differentially abundant in cows exposed to HS relative to HS-free. Database searches combined with GO and KEGG pathway enrichment analyses revealed that many components of the complement and coagulation cascades were altered in heat-stressed cows compared with HS-free cows. Of these, many factors in the complement system (including complement components C1, C3, C5, C6, C7, C8, and C9, complement factor B, and factor H) were down-regulated by HS, while components of the coagulation system (including coagulation factors, vitamin K-dependent proteins, and fibrinogens) were up-regulated by HS. In conclusion, our results indicate that HS decreases plasma levels of complement system proteins, suggesting that immune function is impaired in dairy cows exposed to HS. Though many aspects of heat stress (HS) have been extensively researched, relatively little is known about the proteomics profile changes that occur during heat exposure. In this work, we employed a proteomics approach to investigate differential abundance of plasma proteins in HS-free and heat-stressed dairy cows. Database searches combined with GO and KEGG pathway enrichment analyses revealed that HS resulted in a decrease in complement components, suggesting that heat-stressed dairy cows have impaired immune function. In addition, through integrative analyses of proteomics and previous metabolomics, we showed enhanced glycolysis, lipid metabolic pathway shifts, and nitrogen repartitioning in dairy cows exposed to HS. Our findings expand our current knowledge on the effects of HS on plasma proteomics in dairy cows and offer a new perspective for future research. Copyright © 2016 Elsevier B.V. All rights reserved.

USE OF THE SDO POINTING CONTROLLERS FOR INSTRUMENT CALIBRATION MANEUVERS

NASA Technical Reports Server (NTRS)

Vess, Melissa F.; Starin, Scott R.; Morgenstern, Wendy M.

2005-01-01

During the science phase of the Solar Dynamics Observatory mission, the three science instruments require periodic instrument calibration maneuvers with a frequency of up to once per month. The command sequences for these maneuvers vary in length from a handful of steps to over 200 steps, and individual steps vary in size from 5 arcsec per step to 22.5 degrees per step. Early in the calibration maneuver development, it was determined that the original attitude sensor complement could not meet the knowledge requirements for the instrument calibration maneuvers in the event of a sensor failure. Because the mission must be single fault tolerant, an attitude determination trade study was undertaken to determine the impact of adding an additional attitude sensor versus developing alternative, potentially complex, methods of performing the maneuvers in the event of a sensor failure. To limit the impact to the science data capture budget, these instrument calibration maneuvers must be performed as quickly as possible while maintaining the tight pointing and knowledge required to obtain valid data during the calibration. To this end, the decision was made to adapt a linear pointing controller by adjusting gains and adding an attitude limiter so that it would be able to slew quickly and still achieve steady pointing once on target. During the analysis of this controller, questions arose about the stability of the controller during slewing maneuvers due to the combination of the integral gain, attitude limit, and actuator saturation. Analysis was performed and a method for disabling the integral action while slewing was incorporated to ensure stability. A high fidelity simulation is used to simulate the various instrument calibration maneuvers.

Rapid characterization of seismic sources in Chile: Contribution of the GNSS component

NASA Astrophysics Data System (ADS)

Barrientos, S. E.; Riquelme, S.; Baez, J. C., Sr.

2017-12-01

The recently created National Seismological Center (CSN) of the University of Chile was tasked to upgrade the countrýs seismic network in 2013. The upgrade included new 65 collocated accelerometer and broadband instruments together with 130 GNSS devices designed to transmit their data in real time. Forty units of the GNSS devices include the RTX option, a real time 1-Hz positioning capability at 4-cm error level. The observation system is complemented with 297 additional stand-alone strong motion instruments mainly located in basins for seismic engineering purposes. Broadband data can be accessed in real time from IRIS Data Management Service under networks C and C1. Strong motion event data can be retrieved through the CSN database (evtdb.csn.uchile.cl). A server is being established to handle GNSS data requests through an NTRIP Caster. Completion of the connectivity of the GNSS remote units to the main acquisition servers is expected to take place within several months. In addition to the 40 units providing real time positioning through the RTX option, Precise Point Positioning (PPP) algorithms are being tested on the CSN main servers to enable real time estimates every second for all GNSS remote devices. Because of the high earthquake productivity rate in Chile, the RTX system capabilities have been positively tested in two cases already, demonstrating their excellent performance: i) the main aftershock (M7.6) of the April 1, 2014, northern Chile event and ii) the April 24, 2017, (M6.9) event in central Chile. The former produced coastal horizontal static displacements of the order of 30 cm while the latter of the order of 5 cm. In addition to rapid earthquake characterization through static deformation, W-phase displacement waveform inversions are included in the fast analysis providing excellent results. These new applications and methodologies have profoundly impacted the rapid evaluation of the tsunamigenic potential of large earthquakes in the near field.

Intelligent Observation Strategies for Geosynchronous Remote Sensing for Natural Hazards

NASA Astrophysics Data System (ADS)

Moe, K.; Cappelaere, P. G.; Frye, S. W.; LeMoigne, J.; Mandl, D.; Flatley, T.; Geist, A.

2015-12-01

Geosynchronous satellites offer a unique perspective for monitoring environmental factors important to understanding natural hazards and supporting the disasters management life cycle, namely forecast, detection, response, recovery and mitigation. In the NASA decadal survey for Earth science, the GEO-CAPE mission was proposed to address coastal and air pollution events in geosynchronous orbit, complementing similar initiatives in Asia by the South Koreans and by ESA in Europe, thereby covering the northern hemisphere. In addition to analyzing the challenges of identifying instrument capabilities to meet the science requirements, and the implications of hosting the instrument payloads on commercial geosynchronous satellites, the GEO-CAPE mission design team conducted a short study to explore strategies to optimize the science return for the coastal imaging instrument. The study focused on intelligent scheduling strategies that took into account cloud avoidance techniques as well as onboard processing methods to reduce the data storage and transmission loads. This paper expands the findings of that study to address the use of intelligent scheduling techniques and near-real time data product acquisition of both the coastal water and air pollution events. The topics include the use of onboard processing to refine and execute schedules, to detect cloud contamination in observations, and to reduce data handling operations. Analysis of state of the art flight computing capabilities will be presented, along with an assessment of cloud detection algorithms and their performance characteristics. Tools developed to illustrate operational concepts will be described, including their applicability to environmental monitoring domains with an eye to the future. In the geostationary configuration, the payload becomes a networked "thing" with enough connectivity to exchange data seamlessly with users. This allows the full field of view to be sensed at very high rate under the control of ground infrastructure, resulting in improved efficiencies, accuracy and science benefits. Hence a remote sensing payload and its data may become one of millions of connected objects in the emerging Internet of Things (IoT), and be as easily accessible by a user's smart phone as any other smart appliance.

Intelligent Observation Strategies for Geosynchronous Remote Sensing for Natural Hazards

NASA Technical Reports Server (NTRS)

Moe, Karen; Cappleare, Patrice; Frye, Stuart; LeMoigne, Jacqueline; Mandl, Daniel; Flatley, Thomas; Geist, Alessandro

2015-01-01

Geosynchronous satellites offer a unique perspective for monitoring environmental factors important to understanding natural hazards and supporting the disasters management life cycle, namely forecast, detection, response, recovery and mitigation. In the NASA decadal survey for Earth science, the GEO-CAPE mission was proposed to address coastal and air pollution events in geosynchronous orbit, complementing similar initiatives in Asia by the South Koreans and by ESA in Europe, thereby covering the northern hemisphere. In addition to analyzing the challenges of identifying instrument capabilities to meet the science requirements, and the implications of hosting the instrument payloads on commercial geosynchronous satellites, the GEO-CAPE mission design team conducted a short study to explore strategies to optimize the science return for the coastal imaging instrument. The study focused on intelligent scheduling strategies that took into account cloud avoidance techniques as well as onboard processing methods to reduce the data storage and transmission loads. This paper expands the findings of that study to address the use of intelligent scheduling techniques and near-real time data product acquisition of both the coastal water and air pollution events. The topics include the use of onboard processing to refine and execute schedules, to detect cloud contamination in observations, and to reduce data handling operations. Analysis of state of the art flight computing capabilities will be presented, along with an assessment of cloud detection algorithms and their performance characteristics. Tools developed to illustrate operational concepts will be described, including their applicability to environmental monitoring domains with an eye to the future. In the geostationary configuration, the payload becomes a networked thing with enough connectivity to exchange data seamlessly with users. This allows the full field of view to be sensed at very high rate under the control of ground infrastructure, resulting in improved efficiencies, accuracy and science benefits. Hence a remote sensing payload and its data may become one of millions of connected objects in the emerging Internet of Things (IoT), and be as easily accessible by a users smart phone as any other smart appliance.

Mutations participating in interallelic complementation in propionic acidemia

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gravel, R.A.; Akerman, B.R.; Lamhonwah, A.M.

1994-07-01

Deficiency of propionyl-CoA carboxylase (PCC; [alpha][sub 4][beta][sub 4]) results in the rare, autosomal recessive disease propionic acidemia. Cell fusion experiments have revealed two complementation groups, pccA and pccB, corresponding to defects of the PCCA ([alpha]-subunit) and PCCB ([beta]-subunit) genes, respectively. The pccBCC group includes subgroups, pccB and pccC, which are thought to reflect interallelic complementation between certain mutations of the PCCB gene. In this study, the authors have identified the mutations in two pccB, one pccC, and two pccBC cell lines and have deduced those alleles participating in interallelic complementation. One pccB line was a compound hetrozygote of Pro228Leu andmore » Asn536Asp. The latter mutation was also detected in a noncomplementing pccBC line. This leaves Pro228Leu responsible for complementation in the pccB cells. The second pccB line contained an insertional duplication, dupKICK140-143, and a splice mutation IVS+1 G[yields]T, located after Lys466. The authors suggest that the dupKICK mutation is the complementing allele, since the second allele is incompatible with normal splicing. The pccC line studied was homozygous for Arg410Trp, which is necessarily the complementing allele in that line. For a second pccC line, they previously had proposed that [Delta]Ile408 was the complementing allele. They now show that its second allele, [open quotes]Ins[center dot]Del[close quotes], a 14-bp deletion replaced by a 12-bp insertion beginning at codon 407, fails to complement in homozygous form. The authors conclude that the interallelic complementation results from mutations in domains that can interact between [beta]-subunits in the PCC heteromer to restore enzymatic function. On the basis of sequence homology with the Propionibacterium shermanii transcarboxylase 12S subunit, they suggest that the pccC domain, defined by Ile408 and Arg410, may involve the propionyl-CoA binding site. 37 refs., 5 figs., 2 tabs.« less

The Microbiome and Complement Activation: A Mechanistic Model for Preterm Birth

PubMed Central

Dunn, Alexis B.; Dunlop, Anne L.; Hogue, Carol J.; Miller, Andrew; Corwin, Elizabeth J.

2018-01-01

Preterm Birth (PTB, < 37 completed weeks' gestation) is one of the leading obstetrical problems in the United States affecting approximately 1 of every 9 births. Even more concerning are the persistent racial disparities in PTB with particularly high rates in African Americans. There are several recognized pathophysiologic pathways to PTB, including infection and/or exaggerated systemic or local inflammation. Intrauterine infection is a causal factor linked to PTB, thought to result most commonly from inflammatory processes triggered by microbial invasion of bacteria ascending from the vaginal microbiome. Trials to treat various infections have shown limited efficacy in reducing PTB risk, suggesting that other complex mechanisms, including those associated with inflammation, may be involved in the relationship between microbes, infection, and PTB. A key mediator of the inflammatory response, and recently shown to be associated with PTB, is the complement system, an innate defense mechanism involved in both normal physiologic processes that occur during pregnancy implantation, as well as processes that promote the elimination of pathogenic microbes. The purpose of this paper is to present a mechanistic model of inflammation-associated PTB, which hypothesizes a relationship between the microbiome and dysregulation of the complement system. Exploring the relationships between the microbial environment and complement biomarkers may elucidate a potentially modifiable biological pathway to preterm birth. PMID:28073296

Birth of the Program for Array Seismic Studies of the Continental Lithosphere (PASSCAL)

NASA Astrophysics Data System (ADS)

James, D. E.; Sacks, I. S.

2002-05-01

As recently as 1984 institutions doing portable seismology depended upon their own complement of instruments, almost all designed and built in-house, and all of limited recording capability and flexibility. No data standards existed. Around 1980 the National Research Council (NRC) of the National Academy of Sciences (NAS), with National Science Foundation (NSF) support, empanelled a committee to study a major new initiative in Seismic Studies of the Continental Lithosphere (SSCL). The SSCL report in 1983 recommended that substantial numbers (1000 or more) of new generation digital seismographs be acquired for 3-D high resolution imaging of the continental lithosphere. Recommendations of the SSCL committee dovetailed with other NRC/NAS and NSF reports that highlighted imaging of the continental lithosphere as an area of highest priority. For the first time in the history of portable seismology the question asked was "What do seismologists need to do the job right?" A grassroots effort was undertaken to define instrumentation and data standards for a powerful new set of modern seismic research tools to serve the national seismological community. In the spring and fall of 1983 NSF and IASPEI sponsored workshops were convened to develop specifications for the design of a new generation of portable instrumentation. PASSCAL was the outgrowth of these seminal studies and workshops. The first step toward the formal formation of PASSCAL began with an ad-hoc organizing committee, comprised largely of the members of the NAS lithospheric seismology panel, convened by the authors at Carnegie Institution in Washington in November 1983. From that meeting emerged plans and promises of NSF support for an open organizational meeting to be held in January 1984, in Madison, Wisconsin. By the end of the two-day Madison meeting PASSCAL and an official consortium of seismological institutions for portable seismology were realities. Shortly after, PASSCAL merged with the complementary Global Seismic Network (GSN) under the overall umbrella of the Incorporated Research Institutions for Seismology (IRIS) consortium. Pre-startup funding for PASSCAL was provided by NSF via a so-called "Phase Zero" grant to the Carnegie Institution in June, 1984, to initiate design of new digital instrumentation and to facilitate preparation of the PASSCAL Program Plan. A working group met at Princeton in July 1984 to draft the PASSCAL Program Plan for the IRIS 10-year proposal to NSF, submitted in December 1984. PASSCAL functions as a national facility for seismological research, acquiring and maintaining a large complement of state-of-the-art portable instrumentation for scientists in member institutions. Within a year of its formation, PASSCAL had retained an engineer/program manager and begun the specification process for the manufacture and acquisition of a national instrumentation facility of broadband and short period seismographs. Instrument centers staffed by hardware and software engineers were established to maintain and distribute equipment, and to assist in field installations. By the late 1980s substantial volumes of standardized digital data were flowing from portable experiments to the archives of the newly formed Data Management Center (DMC). Portable broadband sensors built to PASSCAL specifications came on the market in 1989 and transformed the nature of portable experiments by expanding the technical capabilities of portable stations almost to the level of permanent global stations. Today PASSCAL through the instrument center at New Mexico Tech supports dozens of experiments worldwide for high resolution imaging of the earth's interior on all scales.

Gravity gradiometry developments at Lockheed Martin

NASA Astrophysics Data System (ADS)

Difrancesco, D.

2003-04-01

Lockheed Martin has developed and fielded multiple configurations of the rotating accelerometer gravity gradiometer instrument. Applications for both static and moving-base measurements have been demonstrated for a variety of scenarios, including vehicle navigation, hydrocarbon exploration, mineral exploration, reservoir monitoring, underground void detection and treaty monitoring and compliance. The most recent systems built by Lockheed Martin extend the performance range of the early 4-accelerometer gradiometers by adding a second complement of four accelerometers. This achieves the benefit of lower instrument noise and improved frequency response (wider bandwidth) for stringent application scenarios. A summary of the gradiometer development history, functional concepts, instrument and system operation, and demonstrated performance will be presented. Development Background The U. S. Air Force Geophysics Laboratory (AFGL; now AFRL) instituted a program in 1982 to develop and field a moving base gradiometer system that could be used both on land and in the air. The result was the Gravity Gradiometer Survey System (GGSS) which first demonstrated the ability to make airborne gravity gradient measurements in 1987 (Jekeli, 1988). At the same time, the U.S. Navy began development of the Gravity Sensors System (GSS) for use on the Fleet Ballistic Submarine Trident II navigation subsystem. This military background paved the way for commercial uses of gravity gradiometry. Both the GSS and GGSS employed a first generation gravity gradiometer instrument (GGI), which was comprised of four accelerometers mounted on a rotating disk. The details of the GGI operation are further described in the work by Gerber and Hofmeyer (Gerber, 1978 and Hofmeyer, 1994). Recent Advancements in Gradiometer Instrumentation With the instrumentation experience gained through such programs as GSS and GGSS, Lockheed Martin embarked upon an ambitious effort in the early 1990's to further improve the performance of the rotating accelerometer gradiometer design. Under funding from the Defense Threat Reduction Agency (DTRA), a "next generation" gradiometer was developed for the specific purpose of identifying treaty-limited items in arms control inspection scenarios. The result was the Arms Control Verification Gravity Gradiometer (ACVGG), which comprised two complements of four accelerometers to provide for lower noise and improved frequency response. Following the advancements made with the ACVGG, Lockheed Martin began development of an airborne gradiometer to be used for mineral exploration. The Airborne Gravity Gradiometer (AGG) is installed into an inertially stabilized platform for use in a geophysical survey aircraft. The AGG has been successfully deployed in the BHP Billiton Falcon™ system (van Leeuwen, 2000) for detection and identification of mineral targets. The most recent gradiometer development by Lockheed Martin is the Land Gradiometer System for time-lapse measurement (4D), designed and tested in 2000. In this configuration, the gradiometer is employed in a static mode, without a stabilized platform. The system positions the gradiometer at unique heading and tilt combinations to reduce the influence of bias drift and the coupling of horizontal gradients into the measurement (Feldman, 2000). The gradiometer is used to make measurements at discrete points in time (typically months apart) to monitor the time-varying signal associated with such processes as steam flooding, water flooding or gas injection for enhanced oil recovery (Talwani, 2001). The system also has been deployed to make 3D surveys over targets of interest. Conclusions Significant advancements in gradiometer instrumentation have been realized in recent years. Instrument and system performance has improved by nearly two orders of magnitude and new applications have emerged that span a broad range of geophysical interest. References (1)Jekeli, C., 1988, "The Gravity Gradiometer Survey System (GGSS)", EOS, 69, 105 and 116-117 (2)Gerber, M.A., 1978, "Gravity gradiometry - something new in inertial navigation", Astronautics &Aeronautics, 18-26. (3)Hofmeyer, G.M. and Affleck, C.A., 1994, "Rotating Accelerometer Gradiometer", US Patent 5,357,802. (4)Van Leeuwen, E.H., 2000, "BHP develops world's first airborne gravity gradiometer for mineral exploration", Preview 86, 28-30. (5)Feldman, W.K., et al, 2000, "System and Process for Optimizing Gravity Gradiometer Measurements", US Patent 6,125,698. (6)Talwani, M., et al, 2001, "System enables time lapse gradiometry", American Oil &Gas Reporter 44, 101-108

Translational Mini-Review Series on Complement Factor H: Structural and functional correlations for factor H

PubMed Central

Schmidt, C Q; Herbert, A P; Hocking, H G; Uhrín, D; Barlow, P N

2008-01-01

The 155-kDa glycoprotein, complement factor H (CFH), is a regulator of complement activation that is abundant in human plasma. Three-dimensional structures of over half the 20 complement control protein (CCP) modules in CFH have been solved in the context of single-, double- and triple-module segments. Proven binding sites for C3b occupy the N and C termini of this elongated molecule and may be brought together by a bend in CFH mediated by its central CCP modules. The C-terminal CCP 20 is key to the ability of the molecule to adhere to polyanionic markers on self-surfaces where CFH acts to regulate amplification of the alternative pathway of complement. The surface patch on CCP 20 that binds to model glycosaminoglycans has been mapped using nuclear magnetic resonance (NMR), as has a second glycosaminoglycan-binding patch on CCP 7. These patches include many of the residue positions at which sequence variations have been linked to three complement-mediated disorders: dense deposit disease, age-related macular degeneration and atypical haemolytic uraemic syndrome. In one plausible model, CCP 20 anchors CFH to self-surfaces via a C3b/polyanion composite binding site, CCP 7 acts as a ‘proof-reader’ to help discriminate self- from non-self patterns of sulphation, and CCPs 1–4 disrupt C3/C5 convertase formation and stability. PMID:18081691

Children's understanding of the addition/subtraction complement principle.

PubMed

Torbeyns, Joke; Peters, Greet; De Smedt, Bert; Ghesquière, Pol; Verschaffel, Lieven

2016-09-01

In the last decades, children's understanding of mathematical principles has become an important research topic. Different from the commutativity and inversion principles, only few studies have focused on children's understanding of the addition/subtraction complement principle (if a - b = c, then c + b = a), mainly relying on verbal techniques. This contribution aimed at deepening our understanding of children's knowledge of the addition/subtraction complement principle, combining verbal and non-verbal techniques. Participants were 67 third and fourth graders (9- to 10-year-olds). Children solved two tasks in which verbal reports as well as accuracy and speed data were collected. These two tasks differed only in the order of the problems and the instructions. In the looking-back task, children were told that sometimes the preceding problem might help to answer the next problem. In the baseline task, no helpful preceding items were offered. The looking-back task included 10 trigger-target problem pairs on the complement relation. Children verbally reported looking back on about 40% of all target problems in the looking-back task; the target problems were also solved faster and more accurately than in the baseline task. These results suggest that children used their understanding of the complement principle. The verbal and non-verbal data were highly correlated. This study complements previous work on children's understanding of mathematical principles by highlighting interindividual differences in 9- to 10-year-olds' understanding of the complement principle and indicating the potential of combining verbal and non-verbal techniques to investigate (the acquisition of) this understanding. © 2016 The British Psychological Society.

Neutron optics concept for the materials engineering diffractometer at the ESS

NASA Astrophysics Data System (ADS)

Šaroun, J.; Fenske, J.; Rouijaa, M.; Beran, P.; Navrátil, J.; Lukáš, P.; Schreyer, A.; Strobl, M.

2016-09-01

The Beamline for European Materials Engineering Research (BEER) has been recently proposed to be built at the European Spallation Source (ESS). The presented concept of neutron delivery optics for this instrument addresses the problems of bi-spectral beam extraction from a small moderator, optimization of neutron guides profile for long-range neutron transport and focusing at the sample under various constraints. They include free space before and after the guides, a narrow guide section with gaps for choppers, closing of direct line of sight and cost reduction by optimization of the guides cross-section and coating. A system of slits and exchangeable focusing optics is proposed in order to match various wavelength resolution options provided by the pulse shaping and modulation choppers, which permits to efficiently trade resolution for intensity in a wide range. Simulated performance characteristics such as brilliance transfer ratio are complemented by the analysis of the histories of “useful” neutrons obtained by back tracing neutrons hitting the sample, which helps to optimize some of the neutron guide parameters such as supermirror coating.

Atlas of albedo and absorbed solar radiation derived from Nimbus 7 earth radiation budget data set, November 1985 to October 1987

NASA Technical Reports Server (NTRS)

Smith, G. Louis; Rutan, David; Bess, T. Dale

1992-01-01

An atlas of monthly mean global contour maps of albedo and absorbed solar radiation is presented for 21 months from Nov. 1985 to Oct. 1987. These data were retrieved from measurements made by the shortwave wide-field-of-view radiometer of the Earth Radiation Budget (ERB) instrument aboard the Nimbus 7 spacecraft. Profiles of zonal mean albedos and absorbed solar radiation were tabulated. These geographical distributions are provided as a resource for researchers studying the radiation budget of the Earth. The El Nino/Southern Oscillation event of 1986-1987 is included in this data set. This atlas of albedo and absorbed solar radiation extends to 12 years the period covered by two similar atlases: NASA RP-1230 (Jul. 1975 - Oct. 1978) and NASA RP-1231 (Nov. 1978 - Oct. 1985). These three compilations complement the atlases of outgoing longwave radiation by Bess and Smith in NASA RP-1185, RP-1186, and RP-1261, which were also based on the Nimbus 6 and 7 ERB data.

Work-related musculoskeletal complaints: some ergonomics challenges upon the start of a new century.

PubMed

Westgaard, R H

2000-12-01

Three themes likely to be important within health-related ergonomics in the coming years are discussed. The first two themes concern methods for risk analysis of low-level biomechanical and psychosocial exposures. The third theme is approaches to successful implementation of ergonomics interventions. Evidence on the assessment of low-level biomechanical and psychosocial exposures by instrumented measurements is discussed. It is concluded that, despite recent advances in our understanding of exposure-effect associations under these exposure conditions, we must at present rely on more subjective methods, employed in a collaboration between expert and worker. This approach to risk analysis identifies in most cases critical exposures in a work situation. The focus should then be on the successful implementation of measures against those exposures, as identification alone does not solve problems. The aim of improved health for the workers further requires that the full complement of risk factors be considered, including work, leisure time and person-based risk factors. Finally, the need to put ergonomics intervention initiatives in an organisational context is emphasised, and examples of approaches used by Norwegian companies are presented.

Attitude Control System Design for the Solar Dynamics Observatory

NASA Technical Reports Server (NTRS)

Starin, Scott R.; Bourkland, Kristin L.; Kuo-Chia, Liu; Mason, Paul A. C.; Vess, Melissa F.; Andrews, Stephen F.; Morgenstern, Wendy M.

2005-01-01

The Solar Dynamics Observatory mission, part of the Living With a Star program, will place a geosynchronous satellite in orbit to observe the Sun and relay data to a dedicated ground station at all times. SDO remains Sun- pointing throughout most of its mission for the instruments to take measurements of the Sun. The SDO attitude control system is a single-fault tolerant design. Its fully redundant attitude sensor complement includes 16 coarse Sun sensors, a digital Sun sensor, 3 two-axis inertial reference units, 2 star trackers, and 4 guide telescopes. Attitude actuation is performed using 4 reaction wheels and 8 thrusters, and a single main engine nominally provides velocity-change thrust. The attitude control software has five nominal control modes-3 wheel-based modes and 2 thruster-based modes. A wheel-based Safehold running in the attitude control electronics box improves the robustness of the system as a whole. All six modes are designed on the same basic proportional-integral-derivative attitude error structure, with more robust modes setting their integral gains to zero. The paper details the mode designs and their uses.

SEEDS Moving Groups and CHARIS Status Updates

NASA Technical Reports Server (NTRS)

McElwain, Michael

2012-01-01

We present the status update for the SEEDS Moving Groups category. To date, we have observed 59 targets and currently have more than 20 candidates. We also present the expected scientific capabilities of CHARIS, the Coronagraphic High Angular Resolution Imaging Spectrograph, which is being built for the Subaru 8.2 m telescope of the National Astronomical Observatory of Japan. CHARIS will be implemented behind the new extreme adaptive optics system at Subaru, SCExAO, and the existing 188-actuator system AO188. CHARIS will offer three observing modes over nearinfrared wavelengths from 0.9 to 2.4 microns (the y-, J-, H-, and K-bands), including a low-spectral-resolution mode covering this entire wavelength range and a high-resolution mode within a single band. With these capabilities, CHARIS will offer exceptional sensitivity for discovering giant exoplanets, and will enable detailed characterization of their atmospheres, CHARIS, the only planned high-contrast integral field spectrograph on an 8m-class telescope in the Northern Hemisphere, will complement the similar instruments such as Project 1640 at Palomar, and GPI and SPHERE in Chile.

The Stratospheric Observatory for Infrared Astronomy (sofia)

NASA Astrophysics Data System (ADS)

Gehrz, R. D.; Becklin, E. E.

2012-06-01

The joint U.S. and German Stratospheric Observatory for Infrared Astronomy (SOFIA) is a 2.5- meter infrared airborne telescope in a Boeing 747-SP. SOFIA can conduct photometric, spectroscopic, and imaging observations at wavelengths from 0.3 microns to 1.6 millimeters. At SOFIA's maximum service ceiling of 45,000 feet, the average transmission at these wavelengths is greater than 80 percent. SOFIA flys out of the NASA Dryden Flight Research Center aircraft operations facility at Palmdale, CA and the SOFIA Science Mission Operations (SMO) Center is located at NASA Ames Research Center, Moffett Field, CA. SOFIA's first-generation instrument complement includes broadband imagers and spectrographs that can resolve spectral features due to dust and large molecules, and high resolution spectrometers facilitating kinematic studies of molecular and atomic gas lines at km/s resolution. More than 30 science flights of 10 hours length (take-off to landing) were conducted in the past year. About 100 eight to ten hour flights per year are planned by 2014, and the observatory will operate until the mid-2030's.

Sea surface temperature and salinity from French research vessels, 2001–2013

PubMed Central

Gaillard, Fabienne; Diverres, Denis; Jacquin, Stéphane; Gouriou, Yves; Grelet, Jacques; Le Menn, Marc; Tassel, Joelle; Reverdin, Gilles

2015-01-01

French Research vessels have been collecting thermo-salinometer (TSG) data since 1999 to contribute to the Global Ocean Surface Underway Data (GOSUD) programme. The instruments are regularly calibrated and continuously monitored. Water samples are taken on a daily basis by the crew and later analysed in the laboratory. We present here the delayed mode processing of the 2001–2013 dataset and an overview of the resulting quality. Salinity measurement error was a few hundredths of a unit or less on the practical salinity scale (PSS), due to careful calibration and instrument maintenance, complemented with a rigorous adjustment on water samples. In a global comparison, these data show excellent agreement with an ARGO-based salinity gridded product. The Sea Surface Salinity and Temperature from French REsearch SHips (SSST-FRESH) dataset is very valuable for the ‘calibration and validation’ of the new satellite observations delivered by the Soil Moisture and Ocean Salinity (SMOS) and Aquarius missions. PMID:26504523

In Situ Detection of Organic Molecules on the Martian Surface With the Mars Organic Molecule Analyzer (MOMA) on Exomars 2018

NASA Technical Reports Server (NTRS)

Li, Xiang; Brinckerhoff, William B.; Pinnick, Veronica T; van Amerom, Friso H. W.; Danell, Ryan M.; Arevalo, Ricardo D., Jr.; Getty, Stephanie; Mahaffy, Paul R.

2015-01-01

The Mars Organic Molecule Analyzer (MOMA) investigation on the 2018 ExoMars rover will examine the chemical composition of samples acquired from depths of up to two meters below the martian surface, where organics may be protected from radiative and oxidative degradation. The MOMA instrument is centered around a miniaturized linear ion trap (LIT) that facilitates two modes of operation: i) pyrolysisgas chromatography mass spectrometry (pyrGC-MS); and, ii) laser desorptionionization mass spectrometry (LDI-MS) at ambient Mars pressures. The LIT also enables the structural characterization of complex molecules via complementary analytical capabilities, such as multi-frequency waveforms (i.e., SWIFT) and tandem mass spectrometry (MSMS). When combined with the complement of instruments in the rovers Pasteur Payload, MOMA has the potential to reveal the presence of a wide range of organics preserved in a variety of mineralogical environments, and to begin to understand the structural character and potential origin of those compounds.

Microgravity acceleration measurement and environment characterization science (17-IML-1)

NASA Technical Reports Server (NTRS)

1992-01-01

The Space Acceleration Measurement System (SAMS) is a general purpose instrumentation system designed to measure the accelerations onboard the Shuttle Orbiter and Shuttle/Spacelab vehicles. These measurements are used to support microgravity experiments and investigation into the microgravity environment of the vehicle. Acceleration measurements can be made at locations remote from the SAMS main instrumentation unit by the use of up to three remote triaxial sensor heads. The prime objective for SAMS on the International Microgravity Lab (IML-1) mission will be to measure the accelerations experienced by the Fluid Experiment System (FES). The SAMS acceleration measurements for FES will be complemented by low level, low frequency acceleration measurements made by the Orbital Acceleration Research Experiment (OARE) installed on the shuttle. Secondary objectives for SAMS will be to measure accelerations at several specific locations to enable the acceleration transfer function of the Spacelab module to be analyzed. This analysis effort will be in conjunction with similar measurements analyses on other Spacelab missions.

The EOS land validation core sites: background information and current status

USGS Publications Warehouse

Morisette, J.; Privette, J.L.; Justice, C.; Olson, D.; Dwyer, John L.; Davis, P.; Starr, D.; Wickland, D.

1999-01-01

The EOS Land Validation Core Sites1 will provide the user community with timely ground, aircraft, and satellite data for EOS science and validation investigations. The sites, currently 24 distributed worldwide, represent a consensus among the instrument teams and validation investigators and represent a range of global biome types (see Figure 1 and Table 1; Privette et al., 1999; Justice et al., 1998). The sites typically have a history of in situ and remote observations and can expect continued monitoring and land cover research activities. In many cases, a Core Site will have a tower equipped with above-canopy instrumentation for nearcontinuous sampling of landscape radiometric, energy and CO2 flux, meteorological variables, and atmospheric aerosol and water vapor data. These will be complemented by intensive field measurement campaigns. The data collected at these sites will provide an important resource for the broader science community. These sites can also provide a foundation for a validation network supported and used by all international space agencies.

Fast Plasma Instrument for MMS: Data Compression Simulation Results

NASA Astrophysics Data System (ADS)

Barrie, A. C.; Adrian, M. L.; Yeh, P.; Winkert, G. E.; Lobell, J. V.; Viňas, A. F.; Simpson, D. G.; Moore, T. E.

2008-12-01

Magnetospheric Multiscale (MMS) mission will study small-scale reconnection structures and their rapid motions from closely spaced platforms using instruments capable of high angular, energy, and time resolution measurements. To meet these requirements, the Fast Plasma Instrument (FPI) consists of eight (8) identical half top-hat electron sensors and eight (8) identical ion sensors and an Instrument Data Processing Unit (IDPU). The sensors (electron or ion) are grouped into pairs whose 6° × 180° fields-of-view (FOV) are set 90° apart. Each sensor is equipped with electrostatic aperture steering to allow the sensor to scan a 45° × 180° fan about the its nominal viewing (0° deflection) direction. Each pair of sensors, known as the Dual Electron Spectrometer (DES) and the Dual Ion Spectrometer (DIS), occupies a quadrant on the MMS spacecraft and the combination of the eight electron/ion sensors, employing aperture steering, image the full-sky every 30-ms (electrons) and 150-ms (ions), respectively. To probe the diffusion regions of reconnection, the highest temporal/spatial resolution mode of FPI results in the DES complement of a given spacecraft generating 6.5-Mb s-1 of electron data while the DIS generates 1.1-Mb s-1 of ion data yielding an FPI total data rate of 7.6-Mb s-1. The FPI electron/ion data is collected by the IDPU then transmitted to the Central Data Instrument Processor (CIDP) on the spacecraft for science interest ranking. Only data sequences that contain the greatest amount of temporal/spatial structure will be intelligently down-linked by the spacecraft. Currently, the FPI data rate allocation to the CIDP is 1.5-Mb s-1. Consequently, the FPI-IDPU must employ data/image compression to meet this CIDP telemetry allocation. Here, we present simulations of the CCSDS 122.0-B-1 algorithm- based compression of the FPI-DES electron data. Compression analysis is based upon a seed of re- processed Cluster/PEACE electron measurements. Topics to be discussed include: (i) Review of compression algorithm; (ii) Data quality; (iii) Data formatting/organization; (iv) Compression optimization; and (v) Implications for data/matrix pruning. We conclude with a presentation of the base-lined FPI data compression approach.

Use of a structured functional evaluation process for independent medical evaluations of claimants presenting with disabling mental illness: rationale and design for a multi-center reliability study.

PubMed

Bachmann, Monica; de Boer, Wout; Schandelmaier, Stefan; Leibold, Andrea; Marelli, Renato; Jeger, Joerg; Hoffmann-Richter, Ulrike; Mager, Ralph; Schaad, Heinz; Zumbrunn, Thomas; Vogel, Nicole; Bänziger, Oskar; Busse, Jason W; Fischer, Katrin; Kunz, Regina

2016-07-29

Work capacity evaluations by independent medical experts are widely used to inform insurers whether injured or ill workers are capable of engaging in competitive employment. In many countries, evaluation processes lack a clearly structured approach, standardized instruments, and an explicit focus on claimants' functional abilities. Evaluation of subjective complaints, such as mental illness, present additional challenges in the determination of work capacity. We have therefore developed a process for functional evaluation of claimants with mental disorders which complements usual psychiatric evaluation. Here we report the design of a study to measure the reliability of our approach in determining work capacity among patients with mental illness applying for disability benefits. We will conduct a multi-center reliability study, in which 20 psychiatrists trained in our functional evaluation process will assess 30 claimants presenting with mental illness for eligibility to receive disability benefits [Reliability of Functional Evaluation in Psychiatry, RELY-study]. The functional evaluation process entails a five-step structured interview and a reporting instrument (Instrument of Functional Assessment in Psychiatry [IFAP]) to document the severity of work-related functional limitations. We will videotape all evaluations which will be viewed by three psychiatrists who will independently rate claimants' functional limitations. Our primary outcome measure is the evaluation of claimant's work capacity as a percentage (0 to 100 %), and our secondary outcomes are the 12 mental functions and 13 functional capacities assessed by the IFAP-instrument. Inter-rater reliability of four psychiatric experts will be explored using multilevel models to estimate the intraclass correlation coefficient (ICC). Additional analyses include subgroups according to mental disorder, the typicality of claimants, and claimant perceived fairness of the assessment process. We hypothesize that a structured functional approach will show moderate reliability (ICC ≥ 0.6) of psychiatric evaluation of work capacity. Enrollment of actual claimants with mental disorders referred for evaluation by disability/accident insurers will increase the external validity of our findings. Finding moderate levels of reliability, we will continue with a randomized trial to test the reliability of a structured functional approach versus evaluation-as-usual.

THERESA FRANCO INSPECTS THE SOLAR PANELS OF THE MARS GLOBAL SURVEYOR

NASA Technical Reports Server (NTRS)

1996-01-01

Theresa Franco of SPECTROLAB Inc. carefully inspects the solar panels of the Mars Global Surveyor spacecraft, undergoing preflight assembly and checkout in the Payload Hazardous Servicing Facility in KSC's Industrial Area. The four solar array panels will play a crucial role in the Mars Global Surveyor mission by providing the electrical power required to operate the spacecraft and its complement of scientific instruments. The Surveyor is slated for launch November 6 aboard a Delta II expendable launch vehicle. After arriving at the Red Planet in September 1997, the Surveyor will carry out an extensive study of Mars, gathering data about the planet's topography, magnetism, mineral composition and atmosphere.

A second-generation blood substitute (perfluorodichlorooctane emulsion) does not activate complement during an ex vivo circulation model of bypass.

PubMed

Rosoff, J D; Soltow, L O; Vocelka, C R; Schmer, G; Chandler, W L; Cochran, R P; Kunzelman, K S; Spiess, B D

1998-08-01

To examine whether a second-generation perfluorocarbon (PFC) blood substitute added to the cardiopulmonary bypass (CPB) prime influences complement production. A prospective, randomized, single-blinded, ex vivo model. A university hospital, laboratory, and clinics. Ten healthy adult consented volunteer blood donors (five men, five women). Ex vivo closed-loop extracorporeal circuit including membrane oxygenator, tubing, and filter primed with crystalloid or crystalloid plus PFC was circulated for 1 hour with the addition of 500 mL of heparinized fresh human whole blood. Laboratory specimens were drawn from the circuit at 10-minute intervals for 1 hour and measured for complement (C3a, Bb fragment) concentrations, blood gases, fibrinogen concentration, platelet count, and hematocrit. In the PFC group, C3a and Bb fragments were equal to or less than those in the group that received crystalloid alone. The second-generation PFC added to the prime of a CPB circuit does not independently increase complement production.

A Viral Receptor Complementation Strategy to Overcome CAV-2 Tropism for Efficient Retrograde Targeting of Neurons.

PubMed

Li, Shu-Jing; Vaughan, Alexander; Sturgill, James Fitzhugh; Kepecs, Adam

2018-06-06

Retrogradely transported neurotropic viruses enable genetic access to neurons based on their long-range projections and have become indispensable tools for linking neural connectivity with function. A major limitation of viral techniques is that they rely on cell-type-specific molecules for uptake and transport. Consequently, viruses fail to infect variable subsets of neurons depending on the complement of surface receptors expressed (viral tropism). We report a receptor complementation strategy to overcome this by potentiating neurons for the infection of the virus of interest-in this case, canine adenovirus type-2 (CAV-2). We designed AAV vectors for expressing the coxsackievirus and adenovirus receptor (CAR) throughout candidate projection neurons. CAR expression greatly increased retrograde-labeling rates, which we demonstrate for several long-range projections, including some resistant to other retrograde-labeling techniques. Our results demonstrate a receptor complementation strategy to abrogate endogenous viral tropism and thereby facilitate efficient retrograde targeting for functional analysis of neural circuits. Copyright © 2018 Elsevier Inc. All rights reserved.

Correction of xeroderma pigmentosum complementation group D mutant cell phenotypes by chromosome and gene transfer: Involvement of the human ERCC2 DNA repair gene

DOE Office of Scientific and Technical Information (OSTI.GOV)

Flejter, W.L.; McDaniel, L.D.; Johns, D.

1992-01-01

Cultured cells from individuals afflicted with the genetically heterogeneous autosomal recessive disorder xeroderma pigmentosum (XP) exhibit sensitivity to UV radiation and defective nucleotide excision repair. Complementation of these mutant phenotypes after the introduction of single human chromosomes from repair-proficient cells into XP cells has provided a means of mapping the genes involved in this disease. The authors now report the phenotypic correction of XP cells from genetic complementation group D (XP-D) by a single human chromosome designated Tneo. Detailed molecular characterization of Tneo revealed a rearranged structure involving human chromosomes 16 and 19, including the excision repair cross-complementing 2 (ERCC2)more » gene from the previously described human DNA repair gene cluster at 19q13.2-q13.3. Direct transfer of a cosmid bearing the ERCC2 gene conferred UV resistance to XP-D cells.« less

Metabolic Complementation in Bacterial Communities: Necessary Conditions and Optimality

PubMed Central

Mori, Matteo; Ponce-de-León, Miguel; Peretó, Juli; Montero, Francisco

2016-01-01

Bacterial communities may display metabolic complementation, in which different members of the association partially contribute to the same biosynthetic pathway. In this way, the end product of the pathway is synthesized by the community as a whole. However, the emergence and the benefits of such complementation are poorly understood. Herein, we present a simple model to analyze the metabolic interactions among bacteria, including the host in the case of endosymbiotic bacteria. The model considers two cell populations, with both cell types encoding for the same linear biosynthetic pathway. We have found that, for metabolic complementation to emerge as an optimal strategy, both product inhibition and large permeabilities are needed. In the light of these results, we then consider the patterns found in the case of tryptophan biosynthesis in the endosymbiont consortium hosted by the aphid Cinara cedri. Using in-silico computed physicochemical properties of metabolites of this and other biosynthetic pathways, we verified that the splitting point of the pathway corresponds to the most permeable intermediate. PMID:27774085

Preliminary results for an aeromagnetic survey flown over Italy using the HALO (High Altitude and LOng range) research aircraft

NASA Astrophysics Data System (ADS)

Lesur, V.; Gebler, A.; Schachtschneider, R.

2012-12-01

In June 2012 the GEOHALO mission was flown over Italy using the high altitude and long-range German research aircraft HALO (Gulfstream jet - G550). One goal of the mission was to demonstrate the feasibility of using geodetic and geophysical instrumentation on such fast flying aircraft. Several types of data were acquired including gravity, GNSS signals (reflectometry, spectrometry and occultation), laser altimetry and magnetic data. The magnetic data were collected through two independent acquisition chains placed inside under-wing containers. Each chain included a total intensity cesium magnetometer, a three-component fluxgate magnetometer, several temperature censors and a digitizer. Magnetic and temperature data were collected at a 10 Hz sampling rate. Seven parallel profiles, each around 1000 km long, were flown over the Apennine peninsula from north-west to south-east. The flight altitude was about 3500 m and the survey line spacing around 40 km. These long profiles were complemented by four crossing profiles, and a repeated flight line at a higher altitude (approx. 10500 m). The ground speed during the flight was generally around 125 m/s (450 km/h). The output from the first steps of the magnetic data processing will be shown. The measured magnetic data appear to be consistent with the expected signal.

Development of a Microphone Phased Array Capability for the Langley 14- by 22-Foot Subsonic Tunnel

NASA Technical Reports Server (NTRS)

Humphreys, William M.; Brooks, Thomas F.; Bahr, Christopher J.; Spalt, Taylor B.; Bartram, Scott M.; Culliton, William G.; Becker, Lawrence E.

2014-01-01

A new aeroacoustic measurement capability has been developed for use in open-jet testing in the NASA Langley 14- by 22-Foot Subsonic Tunnel (14x22 tunnel). A suite of instruments has been developed to characterize noise source strengths, locations, and directivity for both semi-span and full-span test articles in the facility. The primary instrument of the suite is a fully traversable microphone phased array for identification of noise source locations and strengths on models. The array can be mounted in the ceiling or on either side of the facility test section to accommodate various test article configurations. Complementing the phased array is an ensemble of streamwise traversing microphones that can be placed around the test section at defined locations to conduct noise source directivity studies along both flyover and sideline axes. A customized data acquisition system has been developed for the instrumentation suite that allows for command and control of all aspects of the array and microphone hardware, and is coupled with a comprehensive data reduction system to generate information in near real time. This information includes such items as time histories and spectral data for individual microphones and groups of microphones, contour presentations of noise source locations and strengths, and hemispherical directivity data. The data acquisition system integrates with the 14x22 tunnel data system to allow real time capture of facility parameters during acquisition of microphone data. The design of the phased array system has been vetted via a theoretical performance analysis based on conventional monopole beamforming and DAMAS deconvolution. The performance analysis provides the ability to compute figures of merit for the array as well as characterize factors such as beamwidths, sidelobe levels, and source discrimination for the types of noise sources anticipated in the 14x22 tunnel. The full paper will summarize in detail the design of the instrumentation suite, the construction of the hardware system, and the results of the performance analysis. Although the instrumentation suite is designed to characterize noise for a variety of test articles in the 14x22 tunnel, this paper will concentrate on description of the instruments for two specific test campaigns in the facility, namely a full-span NASA Hybrid Wing Body (HWB) model entry and a semi-span Gulfstream aircraft model entry, tested in the facility in the winter of 2012 and spring of 2013, respectively.

Pass-transistor very large scale integration

NASA Technical Reports Server (NTRS)

Maki, Gary K. (Inventor); Bhatia, Prakash R. (Inventor)

2004-01-01

Logic elements are provided that permit reductions in layout size and avoidance of hazards. Such logic elements may be included in libraries of logic cells. A logical function to be implemented by the logic element is decomposed about logical variables to identify factors corresponding to combinations of the logical variables and their complements. A pass transistor network is provided for implementing the pass network function in accordance with this decomposition. The pass transistor network includes ordered arrangements of pass transistors that correspond to the combinations of variables and complements resulting from the logical decomposition. The logic elements may act as selection circuits and be integrated with memory and buffer elements.

Professionalism Mini-Evaluation Exercise in Finland: A preliminary investigation introducing the Finnish version of the P-MEX instrument.

PubMed

Karukivi, Max; Kortekangas-Savolainen, Outi; Saxén, Ulla; Haapasalo-Pesu, Kirsi-Maria

2015-10-01

Teaching medical professionalism is increasingly acknowledged as an important aspect of medical education. The Professionalism Mini-Evaluation Exercise (P-MEX) is an assessment tool for evaluating medical professionalism, but no studies using it as a self-assessment instrument have been reported. This paper reports on a preliminary investigation of the Finnish version of the P-MEX instrument as an assessment and self-assessment measure. The sample in the present cross-sectional study comprised all 23 medical students and recent graduates (15 females and 8 males) participating in a summer school of psychiatry program in 2014. The two-month program combines clinical work with multifaceted teaching and intensive tutoring. At the end of the program, the participants' medical professionalism was assessed by the tutors and other members of the work community as well as the students themselves using the Finnish version of the P-MEX instrument. The P-MEX scores were compared, using the Friedman test. The mean values and SD for the P-MEX assessments were as follows: tutor assessment 3.26±0.21, work community assessment 3.32±0.26 and self-assessment 3.01±0.07. No significant gender differences were observed. The tutor and work community assessments were significantly correlated (r=0.573, p=0.040), but the self-assessment scores did not correlate with either of the other assessments. Overall, the students evaluated their skills significantly poorer in comparison to the other assessments. Although the small sample size limits the generalization of these preliminary results, the Finnish version of the P-MEX instrument appears to be a feasible measure of medical professionalism. The instrument can also be used as a self-assessment instrument, but subjective evaluations should be complemented with external assessments or feedback in order to take individual and cultural aspects into account.

A Novel Miniature Wide-band Radiometer for Space Applications

NASA Astrophysics Data System (ADS)

Sykulska-Lawrence, H. M.

2016-12-01

Design, development and testing of a novel miniaturised infrared radiometer is described. The instrument opens up new possibilities in planetary science of deployment on smaller platforms - such as unmanned aerial vehicles and microprobes - to enable study of a planet's radiation balance, as well as terrestrial volcano plumes and trace gases in planetary atmospheres, using low-cost long-term observations. Thus a key enabling development is that of miniaturised, low-power and well-calibrated instrumentation. The talk reports advances in miniature technology to perform high accuracy visible / IR remote sensing measurements. The infrared radiometer is akin to those widely used for remote sensing for earth and space applications, which are currently either large instruments on orbiting platforms or medium-sized payloads on balloons. We use MEMS microfabrication techniques to shrink a conventional design, while combining the calibration benefits of large (>1kg) type radiometers with the flexibility and portability of a <10g device. The instrument measures broadband (0.2 to 100µm) upward and downward radiation fluxes, showing improvements in calibration stability and accuracy,with built-in calibration capability, incorporating traceability to temperature standards such as ITS-90. The miniature instrument described here was derived from a concept developed for a European Space Agency study, Dalomis (Proc. of 'i-SAIRAS 2005', Munich, 2005), which involved dropping multiple probes into the atmosphere of Venus from a balloon to sample numerous parts of the complex weather systems on the planet. Data from such an in-situ instrument would complement information from a satellite remote sensing instrument or balloon radiosonde. Moreover, the addition of an internal calibration standard facilitates comparisons between datasets. One of the main challenges for a reduced size device is calibration. We use an in-situ method whereby a blackbody source is integrated within the device and a micromirror switches the input to the detector between the measured signal and the calibration target. Achieving two well-calibrated radiometer channels within a small (<10g) payload is made possible by using modern micromachining techniques.

A Novel Miniature Wide-band Radiometer for Space Applications

NASA Astrophysics Data System (ADS)

Sykulska-Lawrence, Hanna

2016-10-01

Design, development and testing of a novel miniaturised infrared radiometer is described. The instrument opens up new possibilities in planetary science of deployment on smaller platforms - such as unmanned aerial vehicles and microprobes - to enable study of a planet's radiation balance, as well as terrestrial volcano plumes and trace gases in planetary atmospheres, using low-cost long-term observations. Thus a key enabling development is that of miniaturised, low-power and well-calibrated instrumentation.The paper reports advances in miniature technology to perform high accuracy visible / IR remote sensing measurements. The infrared radiometer is akin to those widely used for remote sensing for earth and space applications, which are currently either large instruments on orbiting platforms or medium-sized payloads on balloons. We use MEMS microfabrication techniques to shrink a conventional design, while combining the calibration benefits of large (>1kg) type radiometers with the flexibility and portability of a <10g device. The instrument measures broadband (0.2 to 100um) upward and downward radiation fluxes, with built-in calibration capability, incorporating traceability to temperature standards such as ITS-90.The miniature instrument described here was derived from a concept developed for a European Space Agency study, Dalomis (Proc. of 'i-SAIRAS 2005', Munich, 2005), which involved dropping multiple probes into the atmosphere of Venus from a balloon to sample numerous parts of the complex weather systems on the planet. Data from such an in-situ instrument would complement information from a satellite remote sensing instrument or balloon radiosonde. Moreover, the addition of an internal calibration standard facilitates comparisons between datasets.One of the main challenges for a reduced size device is calibration. We use an in-situ method whereby a blackbody source is integrated within the device and a micromirror switches the input to the detector between the measured signal and the calibration target. Achieving two well-calibrated radiometer channels within a small (<10g) payload is made possible by using micromachining techniques.

Identification of C1q as a Binding Protein for Advanced Glycation End Products.

PubMed

Chikazawa, Miho; Shibata, Takahiro; Hatasa, Yukinori; Hirose, Sayumi; Otaki, Natsuki; Nakashima, Fumie; Ito, Mika; Machida, Sachiko; Maruyama, Shoichi; Uchida, Koji

2016-01-26

Advanced glycation end products (AGEs) make up a heterogeneous group of molecules formed from the nonenzymatic reaction of reducing sugars with the free amino groups of proteins. The abundance of AGEs in a variety of age-related diseases, including diabetic complications and atherosclerosis, and their pathophysiological effects suggest the existence of innate defense mechanisms. Here we examined the presence of serum proteins that are capable of binding glycated bovine serum albumin (AGEs-BSA), prepared upon incubation of BSA with dehydroascorbate, and identified complement component C1q subcomponent subunit A as a novel AGE-binding protein in human serum. A molecular interaction analysis showed the specific binding of C1q to the AGEs-BSA. In addition, we identified DNA-binding regions of C1q, including a collagen-like domain, as the AGE-binding site and established that the amount of positive charge on the binding site was the determining factor. C1q indeed recognized several other modified proteins, including acylated proteins, suggesting that the binding specificity of C1q might be ascribed, at least in part, to the electronegative potential of the ligand proteins. We also observed that C1q was involved in the AGEs-BSA-activated deposition of complement proteins, C3b and C4b. In addition, the AGEs-BSA mediated the proteolytic cleavage of complement protein 5 to release C5a. These findings provide the first evidence of AGEs as a new ligand recognized by C1q, stimulating the C1q-dependent classical complement pathway.

Transcriptome Analysis of the Innate Immunity-Related Complement System in Spleen Tissue of Ctenopharyngodon idella Infected with Aeromonas hydrophila

PubMed Central

Dang, Yunfei; Xu, Xiaoyan; Shen, Yubang; Hu, Moyan; Zhang, Meng; Li, Lisen; Lv, Liqun; Li, Jiale

2016-01-01

The grass carp (Ctenopharyngodon idella) is an important commercial farmed herbivorous fish species in China, but is susceptible to Aeromonas hydrophila infections. In the present study, we performed de novo RNA-Seq sequencing of spleen tissue from specimens of a disease-resistant family, which were given intra-peritoneal injections containing PBS with or without a dose of A. hydrophila. The fish were sampled from the control group at 0 h, and from the experimental group at 4, 8, 12, 24, 48 and 72 h. 122.18 million clean reads were obtained from the normalized cDNA libraries; these were assembled into 425,260 contigs and then 191,795 transcripts. Of those, 52,668 transcripts were annotated with the NCBI Nr database, and 41,347 of the annotated transcripts were assigned into 90 functional groups. 20,569 unigenes were classified into six main categories, including 38 secondary KEGG pathways. 2,992 unigenes were used in the analysis of differentially expressed genes (DEGs). 89 of the putative DEGs were related to the immune system and 41 of them were involved in the complement and coagulation cascades pathway. This study provides insights into the complement and complement-related pathways involved in innate immunity, through expression profile analysis of the genomic resources in C. idella. We conclude that complement and complement-related genes play important roles during defense against A. hydrophila infection. The immune response is activated at 4 h after the bacterial injections, indicating that the complement pathways are activated at the early stage of bacterial infection. The study has improved our understanding of the immune response mechanisms in C. idella to bacterial pathogens. PMID:27383749

Systemic human CR2-targeted complement alternative pathway inhibitor ameliorates mouse laser-induced choroidal neovascularization.

PubMed

Rohrer, Bärbel; Coughlin, Beth; Bandyopadhyay, Mausumi; Holers, V Michael

2012-08-01

Genetic associations and the presence of complement components within pathological structures of age-related macular degeneration (AMD) have generated the hypothesis that AMD is caused by chronic local complement activation. Since the majority of activity in the common terminal pathway results from engagement of the amplification loop, the alternative pathway has been proposed as a logical therapeutic target. We recently generated a factor H (fH)-based complement inhibitor (CR2-fH) with the capacity to be "targeted" to sites of complement C3 activation. We asked whether the human therapeutic (TT30) is effective in a mouse model of AMD. Choroidal neovascularization (CNV) was induced by argon laser photocoagulation of Bruch's membrane. Every other day, mice received intravenous injections of TT30 or vehicles, and after 6 days, the presence or absence of CNV and CNV-related changes were evaluated. Area of CNV, photoreceptor cell function, gene expression for complement components and cytokines, vascular endothelial growth factor (VEGF) protein levels, and TT30 bioavailability were determined. CNV development, which has previously been shown to require local complement activation, could be reduced by intravenous TT30 delivery. Specific inhibition of the alternative pathway not only reduced angiogenesis in CNV, but also ameliorated changes in several associated disease-related biomarkers, including diminished retinal function and molecular events known to be involved in AMD such as VEGF production. After intravenous injection, TT30 localized to CNV lesion sites in the retinal pigmented epithelium-choroid. Systemic administration of TT30 was found to reduce CNV pathology. These data may open new avenues for novel systemic AMD treatment strategies.

Complement-mediated bactericidal activity of anti-factor H binding protein monoclonal antibodies against the meningococcus relies upon blocking factor H binding.

PubMed

Giuntini, Serena; Reason, Donald C; Granoff, Dan M

2011-09-01

Binding of the complement-downregulating protein factor H (fH) to the surface of the meningococcus is important for survival of the organism in human serum. The meningococcal vaccine candidate factor H binding protein (fHbp) is an important ligand for human fH. While some fHbp-specific monoclonal antibodies (MAbs) block binding of fH to fHbp, the stoichiometry of blocking in the presence of high serum concentrations of fH and its effect on complement-mediated bactericidal activity are unknown. To investigate this question, we constructed chimeric antibodies in which the human IgG1 constant region was paired with three murine fHbp-specific binding domains designated JAR 3, JAR 5, and MAb502. By surface plasmon resonance, the association rates for binding of all three MAbs to immobilized fHbp were >50-fold higher than that for binding of fH to fHbp, and the MAb dissociation rates were >500-fold lower than that for fH. While all three MAbs elicited similar C1q-dependent C4b deposition on live bacteria (classical complement pathway), only those antibodies that inhibited binding of fH to fHbp (JAR 3 and JAR 5) had bactericidal activity with human complement. MAb502, which did not inhibit fH binding, had complement-mediated bactericidal activity only when tested with fH-depleted human complement. When an IgG1 anti-fHbp MAb binds to sparsely exposed fHbp on the bacterial surface, there appears to be insufficient complement activation for bacteriolysis unless fH binding also is inhibited. The ability of fHbp vaccines to elicit protective antibodies, therefore, is likely to be enhanced if the antibody repertoire is of high avidity and includes fH-blocking activity.

A scabies mite serpin interferes with complement-mediated neutrophil functions and promotes staphylococcal growth.

PubMed

Swe, Pearl M; Fischer, Katja

2014-06-01

Scabies is a contagious skin disease caused by the parasitic mite Sarcoptes scabiei. The disease is highly prevalent worldwide and known to predispose to secondary bacterial infections, in particular by Streptococcus pyogenes and Staphylococcus aureus. Reports of scabies patients co-infected with methicillin resistant S. aureus (MRSA) pose a major concern for serious down-stream complications. We previously reported that a range of complement inhibitors secreted by the mites promoted the growth of S. pyogenes. Here, we show that a recently characterized mite serine protease inhibitor (SMSB4) inhibits the complement-mediated blood killing of S. aureus. Blood killing of S. aureus was measured in whole blood bactericidal assays, counting viable bacteria recovered after treatment in fresh blood containing active complement and phagocytes, treated with recombinant SMSB4. SMSB4 inhibited the blood killing of various strains of S. aureus including methicillin-resistant and methicillin-sensitive isolates. Staphylococcal growth was promoted in a dose-dependent manner. We investigated the effect of SMSB4 on the complement-mediated neutrophil functions, namely phagocytosis, opsonization and anaphylatoxin release, by flow cytometry and in enzyme linked immuno sorbent assays (ELISA). SMSB4 reduced phagocytosis of S. aureus by neutrophils. It inhibited the deposition of C3b, C4b and properdin on the bacteria surface, but did not affect the depositions of C1q and MBL. SMSB4 also inhibited C5 cleavage as indicated by a reduced C5b-9 deposition. We postulate that SMSB4 interferes with the activation of all three complement pathways by reducing the amount of C3 convertase formed. We conclude that SMSB4 interferes with the complement-dependent killing function of neutrophils, thereby reducing opsonization, phagocytosis and further recruitment of neutrophils to the site of infection. As a consequence secreted scabies mites complement inhibitors, such as SMSB4, provide favorable conditions for the onset of S. aureus co-infection in the scabies-infected microenvironment by suppressing the immediate host immune response.

Hypocomplementemia is associated with worse renal survival in ANCA-positive granulomatosis with polyangiitis and microscopic polyangiitis

PubMed Central

Aouba, Achille; Khoy, Kathy; Mariotte, Delphine; Lobbedez, Thierry; Martin Silva, Nicolas

2018-01-01

Recent data suggest the existence of a complement alternative pathway activation in the pathogenesis of antineutrophilic cytoplasmic antibody (ANCA)-associated vasculitis (AAV), a condition that remains poorly understood. This study aims to assess the clinical characteristics and outcomes of granulomatosis with polyangiitis (GPA) and microscopic polyangiitis (MPA) patients with regard to their plasma complement levels at diagnosis. A retrospective monocentric study carried out at Caen University Hospital led to the identification of proteinase-3- or myeloperoxidase-ANCA-positive GPA and MPA patients from January 2000 to June 2016 and from September 2011 to June 2016, respectively. All patients with available C3 and C4 levels at diagnosis were included. Patients were categorized in the hypocomplementemia group if their C3 and/or C4 levels at diagnosis were below the lower limit of the normal range. Among the 76 AAV patients (43 GPA, 33 MPA), 4 (5%) had hypocomplementemia, and the 72 remaining patients exhibited normal plasma complement levels. All 4 hypocomplementemia patients had renal involvement. Hypocomplementemia was followed in 1 patient whose post-treatment complement level normalized within 1 month. Among all clinical and ANCA specificity, including relapse-free survival (p = 0.093), only overall and renal survival rates were significantly lower in the hypocomplementemia group (p = 0.0011 and p<0.001, respectively). Hypocomplementemia with low C3 and/or C4 levels at GPA or MPA diagnosis may be responsible for worse survival and renal prognosis. These results argue for larger and prospective studies to better determine the epidemiology of the disease and to assess complement-targeting therapy in these patients. PMID:29621352

Efficacy of INSURE during nasal CPAP in preterm infants with respiratory distress syndrome.

PubMed

Leone, F; Trevisanuto, D; Cavallin, F; Parotto, M; Zanardo, V

2013-04-01

INSURE (INtubation, SURfactant, Extubation) is a proven complement of nasal CPAP (nCPAP) for respiratory distress syndrome (RDS) treatment of preterm infants. Early administration is characterized by greater success. We aimed to determine the efficacy and failure or other respiratory outcomes of INSURE administration during nasal continous positive airway pressure (nCPAP) treatment of RDS. Among 824 premature infants neonatal intensive care unit (NICU) admitted at Padua University Hospital during 2007-2009, 209 (25.4%) were managed by surfactant replacement (200 mg/kg, Curosurf®) if required >45% oxygen ("rescue" treatment), including 42 (20.1%) during nCPAP. Each premature infant treated with INSURE during nasal CPAP was compared to 2 consecutive control infants treated with surfactant during mechanical ventilation, matched for antenatal steroids, delivery route, gestational age, and sex. Infants with RDS, treated with nCPAP and INSURE-complement (N.=25), were comparable in Apgar score, need of PPV at birth, birth weight, pre-surfactant FiO2 and timing of surfactant replacement to controls. However, nCPAP and INSURE-complement was superior in terms both of oxygenation, evaluated as post-treatment FiO2 (Median, [IQR], 26 [21-40] vs. 21 [21-29]; P=0.03) and (a-A) pO2 (0.48 [0.45-0.60] vs. 0.58 [0.53-0.72]; P=0.03). The improved oxygenation was sustained over the following days. In addition, premature infants treated with nCPAP and INSURE-complement developed less respiratory co-morbidities, including pneumothorax, borncopulmonary disease (BPD), and BPD and death (P=0.04). INSURE-complement of nasal CPAP has a superior efficacy in terms of oxygenation improvement, maintenance of optimal oxygenation, and reduction of respiratory comorbidities respect to "rescue" surfactant administration during mechanical ventilation.

Complement activation in leprosy: a retrospective study shows elevated circulating terminal complement complex in reactional leprosy.

PubMed

Bahia El Idrissi, N; Hakobyan, S; Ramaglia, V; Geluk, A; Morgan, B Paul; Das, P Kumar; Baas, F

2016-06-01

Mycobacterium leprae infection gives rise to the immunologically and histopathologically classified spectrum of leprosy. At present, several tools for the stratification of patients are based on acquired immunity markers. However, the role of innate immunity, particularly the complement system, is largely unexplored. The present retrospective study was undertaken to explore whether the systemic levels of complement activation components and regulators can stratify leprosy patients, particularly in reference to the reactional state of the disease. Serum samples from two cohorts were analysed. The cohort from Bangladesh included multi-bacillary (MB) patients with (n = 12) or without (n = 46) reaction (R) at intake and endemic controls (n = 20). The cohort from Ethiopia included pauci-bacillary (PB) (n = 7) and MB (n = 23) patients without reaction and MB (n = 15) patients with reaction. The results showed that the activation products terminal complement complex (TCC) (P ≤ 0·01), C4d (P ≤ 0·05) and iC3b (P ≤ 0·05) were specifically elevated in Bangladeshi patients with reaction at intake compared to endemic controls. In addition, levels of the regulator clusterin (P ≤ 0·001 without R; P < 0·05 with R) were also elevated in MB patients, irrespective of a reaction. Similar analysis of the Ethiopian cohort confirmed that, irrespective of a reaction, serum TCC levels were increased significantly in patients with reactions compared to patients without reactions (P ≤ 0·05). Our findings suggests that serum TCC levels may prove to be a valuable tool in diagnosing patients at risk of developing reactions. © 2016 British Society for Immunology.

Novel Scabies Mite Serpins Inhibit the Three Pathways of the Human Complement System

PubMed Central

Mika, Angela; Reynolds, Simone L.; Mohlin, Frida C.; Willis, Charlene; Swe, Pearl M.; Pickering, Darren A.; Halilovic, Vanja; Wijeyewickrema, Lakshmi C.; Pike, Robert N.; Blom, Anna M.; Kemp, David J.; Fischer, Katja

2012-01-01

Scabies is a parasitic infestation of the skin by the mite Sarcoptes scabiei that causes significant morbidity worldwide, in particular within socially disadvantaged populations. In order to identify mechanisms that enable the scabies mite to evade human immune defenses, we have studied molecules associated with proteolytic systems in the mite, including two novel scabies mite serine protease inhibitors (SMSs) of the serpin superfamily. Immunohistochemical studies revealed that within mite-infected human skin SMSB4 (54 kDa) and SMSB3 (47 kDa) were both localized in the mite gut and feces. Recombinant purified SMSB3 and SMSB4 did not inhibit mite serine and cysteine proteases, but did inhibit mammalian serine proteases, such as chymotrypsin, albeit inefficiently. Detailed functional analysis revealed that both serpins interfered with all three pathways of the human complement system at different stages of their activation. SMSB4 inhibited mostly the initial and progressing steps of the cascades, while SMSB3 showed the strongest effects at the C9 level in the terminal pathway. Additive effects of both serpins were shown at the C9 level in the lectin pathway. Both SMSs were able to interfere with complement factors without protease function. A range of binding assays showed direct binding between SMSB4 and seven complement proteins (C1, properdin, MBL, C4, C3, C6 and C8), while significant binding of SMSB3 occurred exclusively to complement factors without protease function (C4, C3, C8). Direct binding was observed between SMSB4 and the complement proteases C1s and C1r. However no complex formation was observed between either mite serpin and the complement serine proteases C1r, C1s, MASP-1, MASP-2 and MASP-3. No catalytic inhibition by either serpin was observed for any of these enzymes. In summary, the SMSs were acting at several levels mediating overall inhibition of the complement system and thus we propose that they may protect scabies mites from complement-mediated gut damage. PMID:22792350

Micrurus snake venoms activate human complement system and generate anaphylatoxins

PubMed Central

2012-01-01

Background The genus Micrurus, coral snakes (Serpentes, Elapidae), comprises more than 120 species and subspecies distributed from the south United States to the south of South America. Micrurus snake bites can cause death by muscle paralysis and further respiratory arrest within a few hours after envenomation. Clinical observations show mainly neurotoxic symptoms, although other biological activities have also been experimentally observed, including cardiotoxicity, hemolysis, edema and myotoxicity. Results In the present study we have investigated the action of venoms from seven species of snakes from the genus Micrurus on the complement system in in vitro studies. Several of the Micrurus species could consume the classical and/or the lectin pathways, but not the alternative pathway, and C3a, C4a and C5a were generated in sera treated with the venoms as result of this complement activation. Micrurus venoms were also able to directly cleave the α chain of the component C3, but not of the C4, which was inhibited by 1,10 Phenanthroline, suggesting the presence of a C3α chain specific metalloprotease in Micrurus spp venoms. Furthermore, complement activation was in part associated with the cleavage of C1-Inhibitor by protease(s) present in the venoms, which disrupts complement activation control. Conclusion Micrurus venoms can activate the complement system, generating a significant amount of anaphylatoxins, which may assist due to their vasodilatory effects, to enhance the spreading of other venom components during the envenomation process. PMID:22248157

Immunological response to nitroglycerin-loaded shear-responsive liposomes in vitro and in vivo.

PubMed

Buscema, Marzia; Matviykiv, Sofiya; Mészáros, Tamás; Gerganova, Gabriela; Weinberger, Andreas; Mettal, Ute; Mueller, Dennis; Neuhaus, Frederik; Stalder, Etienne; Ishikawa, Takashi; Urbanics, Rudolf; Saxer, Till; Pfohl, Thomas; Szebeni, János; Zumbuehl, Andreas; Müller, Bert

2017-10-28

Liposomes formulated from the 1,3-diamidophospholipid Pad-PC-Pad are shear-responsive and thus promising nano-containers to specifically release a vasodilator at stenotic arteries. The recommended preclinical safety tests for therapeutic liposomes of nanometer size include the in vitro assessment of complement activation and the evaluation of the associated risk of complement activation-related pseudo-allergy (CARPA) in vivo. For this reason, we measured complement activation by Pad-PC-Pad formulations in human and porcine sera, along with the nanopharmaceutical-mediated cardiopulmonary responses in pigs. The evaluated formulations comprised of Pad-PC-Pad liposomes, with and without polyethylene glycol on the surface of the liposomes, and nitroglycerin as a model vasodilator. The nitroglycerin incorporation efficiency ranged from 25% to 50%. In human sera, liposome formulations with 20mg/mL phospholipid gave rise to complement activation, mainly via the alternative pathway, as reflected by the rises in SC5b-9 and Bb protein complex concentrations. Formulations having a factor of ten lower phospholipid content did not result in measurable complement activation. The weak complement activation induced by Pad-PC-Pad liposomal formulations was confirmed by the results obtained by performing an in vivo study in a porcine model, where hemodynamic parameters were monitored continuously. Our study suggests that, compared to FDA-approved liposomal drugs, Pad-PC-Pad exhibits less or similar risks of CARPA. Copyright © 2017 The Authors. Published by Elsevier B.V. All rights reserved.

Micrurus snake venoms activate human complement system and generate anaphylatoxins.

PubMed

Tanaka, Gabriela D; Pidde-Queiroz, Giselle; de Fátima D Furtado, Maria; van den Berg, Carmen; Tambourgi, Denise V

2012-01-16

The genus Micrurus, coral snakes (Serpentes, Elapidae), comprises more than 120 species and subspecies distributed from the south United States to the south of South America. Micrurus snake bites can cause death by muscle paralysis and further respiratory arrest within a few hours after envenomation. Clinical observations show mainly neurotoxic symptoms, although other biological activities have also been experimentally observed, including cardiotoxicity, hemolysis, edema and myotoxicity. In the present study we have investigated the action of venoms from seven species of snakes from the genus Micrurus on the complement system in in vitro studies. Several of the Micrurus species could consume the classical and/or the lectin pathways, but not the alternative pathway, and C3a, C4a and C5a were generated in sera treated with the venoms as result of this complement activation. Micrurus venoms were also able to directly cleave the α chain of the component C3, but not of the C4, which was inhibited by 1,10 Phenanthroline, suggesting the presence of a C3α chain specific metalloprotease in Micrurus spp venoms. Furthermore, complement activation was in part associated with the cleavage of C1-Inhibitor by protease(s) present in the venoms, which disrupts complement activation control. Micrurus venoms can activate the complement system, generating a significant amount of anaphylatoxins, which may assist due to their vasodilatory effects, to enhance the spreading of other venom components during the envenomation process.

Plasmin cleaves fibrinogen and the human complement proteins C3b and C5 in the presence of Leptospira interrogans proteins: A new role of LigA and LigB in invasion and complement immune evasion.

PubMed

Castiblanco-Valencia, Mónica Marcela; Fraga, Tatiana Rodrigues; Pagotto, Ana Helena; Serrano, Solange Maria de Toledo; Abreu, Patricia Antonia Estima; Barbosa, Angela Silva; Isaac, Lourdes

2016-05-01

Plasminogen is a single-chain glycoprotein found in human plasma as the inactive precursor of plasmin. When converted to proteolytically active plasmin, plasmin(ogen) regulates both complement and coagulation cascades, thus representing an important target for pathogenic microorganisms. Leptospira interrogans binds plasminogen, which is converted to active plasmin. Leptospiral immunoglobulin-like (Lig) proteins are surface exposed molecules that interact with extracellular matrix components and complement regulators, including proteins of the FH family and C4BP. In this work, we demonstrate that these multifunctional molecules also bind plasminogen through both N- and C-terminal domains. These interactions are dependent on lysine residues and are affected by ionic strength. Competition assays suggest that plasminogen does not share binding sites with C4BP or FH on Lig proteins at physiological molar ratios. Plasminogen bound to Lig proteins is converted to proteolytic active plasmin in the presence of urokinase-type plasminogen activator (uPA). Lig-bound plasmin is able to cleave the physiological substrates fibrinogen and the complement proteins C3b and C5. Taken together, our data point to a new role of LigA and LigB in leptospiral invasion and complement immune evasion. Plasmin(ogen) acquisition by these versatile proteins may contribute to Leptospira infection, favoring bacterial survival and dissemination inside the host. Copyright © 2016. Published by Elsevier GmbH.

Mannose-binding lectin binds to Ebola and Marburg envelope glycoproteins, resulting in blocking of virus interaction with DC-SIGN and complement-mediated virus neutralization.

PubMed

Ji, Xin; Olinger, Gene G; Aris, Sheena; Chen, Ying; Gewurz, Henry; Spear, Gregory T

2005-09-01

Mannose-binding lectin (MBL), a serum lectin that mediates innate immune functions including activation of the lectin complement pathway, binds to carbohydrates expressed on some viral glycoproteins. In this study, the ability of MBL to bind to virus particles pseudotyped with Ebola and Marburg envelope glycoproteins was evaluated. Virus particles bearing either Ebola (Zaire strain) or Marburg (Musoke strain) envelope glycoproteins bound at significantly higher levels to immobilized MBL compared with virus particles pseudotyped with vesicular stomatitis virus glycoprotein or with no virus glycoprotein. As observed in previous studies, Ebola-pseudotyped virus bound to cells expressing the lectin DC-SIGN (dendritic cell-specific intercellular adhesion molecule 3-grabbing non-integrin). However, pre-incubation of virus with MBL blocked DC-SIGN-mediated binding to cells, suggesting that the two lectins bind at the same or overlapping sites on the Ebola glycoprotein. Neutralization experiments showed that virus pseudotyped with Ebola or Marburg (Musoke) glycoprotein was neutralized by complement, while the Marburg (Ravn strain) glycoprotein-pseudotyped virus was less sensitive to neutralization. Neutralization was partially mediated through the lectin complement pathway, since a complement source deficient in MBL was significantly less effective at neutralizing viruses pseudotyped with filovirus glycoproteins and addition of purified MBL to the MBL-deficient complement increased neutralization. These experiments demonstrated that MBL binds to filovirus envelope glycoproteins resulting in important biological effects and suggest that MBL can interact with filoviruses during infection in humans.

The Role of Complement in Cnidarian-Dinoflagellate Symbiosis and Immune Challenge in the Sea Anemone Aiptasia pallida

PubMed Central

Poole, Angela Z.; Kitchen, Sheila A.; Weis, Virginia M.

2016-01-01

The complement system is an innate immune pathway that in vertebrates, is responsible for initial recognition and ultimately phagocytosis and destruction of microbes. Several complement molecules including C3, Factor B, and mannose binding lectin associated serine proteases (MASP) have been characterized in invertebrates and while most studies have focused on their conserved role in defense against pathogens, little is known about their role in managing beneficial microbes. The purpose of this study was to (1) characterize complement pathway genes in the symbiotic sea anemone Aiptasia pallida, (2) investigate the evolution of complement genes in invertebrates, and (3) examine the potential dual role of complement genes Factor B and MASP in the onset and maintenance of cnidarian-dinoflagellate symbiosis and immune challenge using qPCR based studies. The results demonstrate that A. pallida has multiple Factor B genes (Ap_Bf-1, Ap_Bf-2a, and Ap_Bf-2b) and one MASP gene (Ap_MASP). Phylogenetic analysis indicates that the evolutionary history of complement genes is complex, and there have been many gene duplications or gene loss events, even within members of the same phylum. Gene expression analyses revealed a potential role for complement in both onset and maintenance of cnidarian-dinoflagellate symbiosis and immune challenge. Specifically, Ap_Bf-1 and Ap_MASP are significantly upregulated in the light at the onset of symbiosis and in response to challenge with the pathogen Serratia marcescens suggesting that they play a role in the initial recognition of both beneficial and harmful microbes. Ap_Bf-2b in contrast, was generally downregulated during the onset and maintenance of symbiosis and in response to challenge with S. marcescens. Therefore, the exact role of Ap_Bf-2b in response to microbes remains unclear, but the results suggest that the presence of microbes leads to repressed expression. Together, these results indicate functional divergence between Ap_Bf-1 and Ap_Bf-2b, and that Ap_Bf-1 and Ap_MASP may be functioning together in an ancestral hybrid of the lectin and alternative complement pathways. Overall, this study provides information on the role of the complement system in a basal metazoan and its role in host-microbe interactions. PMID:27148208

The Role of Complement in Cnidarian-Dinoflagellate Symbiosis and Immune Challenge in the Sea Anemone Aiptasia pallida.

PubMed

Poole, Angela Z; Kitchen, Sheila A; Weis, Virginia M

2016-01-01

The complement system is an innate immune pathway that in vertebrates, is responsible for initial recognition and ultimately phagocytosis and destruction of microbes. Several complement molecules including C3, Factor B, and mannose binding lectin associated serine proteases (MASP) have been characterized in invertebrates and while most studies have focused on their conserved role in defense against pathogens, little is known about their role in managing beneficial microbes. The purpose of this study was to (1) characterize complement pathway genes in the symbiotic sea anemone Aiptasia pallida, (2) investigate the evolution of complement genes in invertebrates, and (3) examine the potential dual role of complement genes Factor B and MASP in the onset and maintenance of cnidarian-dinoflagellate symbiosis and immune challenge using qPCR based studies. The results demonstrate that A. pallida has multiple Factor B genes (Ap_Bf-1, Ap_Bf-2a, and Ap_Bf-2b) and one MASP gene (Ap_MASP). Phylogenetic analysis indicates that the evolutionary history of complement genes is complex, and there have been many gene duplications or gene loss events, even within members of the same phylum. Gene expression analyses revealed a potential role for complement in both onset and maintenance of cnidarian-dinoflagellate symbiosis and immune challenge. Specifically, Ap_Bf-1 and Ap_MASP are significantly upregulated in the light at the onset of symbiosis and in response to challenge with the pathogen Serratia marcescens suggesting that they play a role in the initial recognition of both beneficial and harmful microbes. Ap_Bf-2b in contrast, was generally downregulated during the onset and maintenance of symbiosis and in response to challenge with S. marcescens. Therefore, the exact role of Ap_Bf-2b in response to microbes remains unclear, but the results suggest that the presence of microbes leads to repressed expression. Together, these results indicate functional divergence between Ap_Bf-1 and Ap_Bf-2b, and that Ap_Bf-1 and Ap_MASP may be functioning together in an ancestral hybrid of the lectin and alternative complement pathways. Overall, this study provides information on the role of the complement system in a basal metazoan and its role in host-microbe interactions.

Complement-Mediated Neutralization of Canine Distemper Virus In Vitro: Cross-Reaction between Vaccine Onderstepoort and Field KDK-1 Strains with Different Hemagglutinin Gene Characteristics

PubMed Central

Mochizuki, Masami; Motoyoshi, Megumi; Maeda, Ken; Kai, Kazunari

2002-01-01

The properties of neutralization of antigens of canine distemper virus Onderstepoort and a recent field isolate, KDK-1, were investigated with strain-specific dog sera. A conventional neutralization assay indicated antigenic dissimilarity between the strains; however, when guinea pig complement was included in the reaction mixture, the strains were neutralized with not only the homologous but also the heterologous antibodies. PMID:12093697

Development of an Educational Game to Set Up Surgical Instruments on the Mayo Stand or Back Table: Applied Research in Production Technology

PubMed Central

2017-01-01

Background Existing research suggests that digital games can be used effectively for educational purposes at any level of training. Perioperative nursing educators can use games to complement curricula, in guidance and staff development programs, to foster team collaboration, and to give support to critical thinking in nursing practice because it is a complex environment. Objective To describe the process of developing an educational game to set up surgical instruments on the Mayo stand or back table as a resource to assist the instructor in surgical instrumentation training for students and nursing health professionals in continued education. Methods The study was characterized by applied research in production technology. It included the phases of analysis and design, development, and evaluation. The objectives of the educational game were developed through Bloom’s taxonomy. Parallel to the physical development of the educational game, a proposed model for the use of digital elements in educational game activities was applied to develop the game content. Results The development of the game called “Playing with Tweezers” was carried out in 3 phases and was evaluated by 15 participants, comprising students and professional experts in various areas of knowledge such as nursing, information technology, and education. An environment was created with an initial screen, menu buttons containing the rules of the game, and virtual tour modes for learning and assessment. Conclusions The “digital” nursing student needs engagement, stimulation, reality, and entertainment, not just readings. “Playing with Tweezers” is an example of educational gaming as an innovative teaching strategy in nursing that encourages the strategy of involving the use of educational games to support theoretical or practical classroom teaching. Thus, the teacher does not work with only 1 type of teaching methodology, but with a combination of different methodologies. In addition, we cannot forget that skill training in an educational game does not replace curricular practice, but helps. PMID:28073736

The FIELDS Instrument Suite on MMS: Scientific Objectives, Measurements, and Data Products

NASA Astrophysics Data System (ADS)

Torbert, R. B.; Russell, C. T.; Magnes, W.; Ergun, R. E.; Lindqvist, P.-A.; Le Contel, O.; Vaith, H.; Macri, J.; Myers, S.; Rau, D.; Needell, J.; King, B.; Granoff, M.; Chutter, M.; Dors, I.; Olsson, G.; Khotyaintsev, Y. V.; Eriksson, A.; Kletzing, C. A.; Bounds, S.; Anderson, B.; Baumjohann, W.; Steller, M.; Bromund, K.; Le, Guan; Nakamura, R.; Strangeway, R. J.; Leinweber, H. K.; Tucker, S.; Westfall, J.; Fischer, D.; Plaschke, F.; Porter, J.; Lappalainen, K.

2016-03-01

The FIELDS instrumentation suite on the Magnetospheric Multiscale (MMS) mission provides comprehensive measurements of the full vector magnetic and electric fields in the reconnection regions investigated by MMS, including the dayside magnetopause and the night-side magnetotail acceleration regions out to 25 Re. Six sensors on each of the four MMS spacecraft provide overlapping measurements of these fields with sensitive cross-calibrations both before and after launch. The FIELDS magnetic sensors consist of redundant flux-gate magnetometers (AFG and DFG) over the frequency range from DC to 64 Hz, a search coil magnetometer (SCM) providing AC measurements over the full whistler mode spectrum expected to be seen on MMS, and an Electron Drift Instrument (EDI) that calibrates offsets for the magnetometers. The FIELDS three-axis electric field measurements are provided by two sets of biased double-probe sensors (SDP and ADP) operating in a highly symmetric spacecraft environment to reduce significantly electrostatic errors. These sensors are complemented with the EDI electric measurements that are free from all local spacecraft perturbations. Cross-calibrated vector electric field measurements are thus produced from DC to 100 kHz, well beyond the upper hybrid resonance whose frequency provides an accurate determination of the local electron density. Due to its very large geometric factor, EDI also provides very high time resolution (˜1 ms) ambient electron flux measurements at a few selected energies near 1 keV. This paper provides an overview of the FIELDS suite, its science objectives and measurement requirements, and its performance as verified in calibration and cross-calibration procedures that result in anticipated errors less than 0.1 nT in B and 0.5 mV/m in E. Summaries of data products that result from FIELDS are also described, as well as algorithms for cross-calibration. Details of the design and performance characteristics of AFG/DFG, SCM, ADP, SDP, and EDI are provided in five companion papers.

The IRM at 25: A Quarter Century of Community-Based Research and Education at the Institute for Rock Magnetism

NASA Astrophysics Data System (ADS)

Moskowitz, B. M.

2015-12-01

A 1986 meeting on the future of rock magnetism proposed an idea for a center where researchers in rock magnetism, other earth science disciplines, and allied fields in the physical sciences could share ideas and have access to advanced instrumentation in magnetism. The idea became reality in 1990, when the Institute for Rock Magnetism (IRM) was established as a shared resource for the GP and broader research communities, providing instruments to study the magnetism of rocks, sediment, biological materials and synthetic analogs. This is accomplished with a suite of instruments that measures magnetization from 2-1000 K, in DC fields up to 5 T and AC fields up to 10 kHz. These are complemented by Mössbauer spectrometers (4.2-300K, 0-6.5T), a high-temperature magnetic force microscope (Tmax~ 673 K), and a low-temperature probe (20-300 K) for vector remanence measurements. A unique aspect of the IRM was that it allowed for routine measurements below 300 K and provided new ways of "seeing" magnetism. This has enabled researchers to study magnetic behavior through magnetic ordering temperatures, crystal phase transitions, and blocking temperatures, providing new insights into mineral magnetism as well as developing new methods to interpret the magnetism of natural materials. The main access to the IRM is the Visiting Fellowship (VF) program, where 379 have been awarded representing 157 institutions from the US and 30 countries. Nearly 50% of VFs have gone to students. The total output of visiting and in-house researches have produced about 800 publications to date. The IRM also provides education and outreach activities including: (1) The IRM Quarterly with over 600 subscribers; (2) The Biennial Santa Fe meetings on the current state and future trends in magnetic research; and (3) The Biennial Summer Schools for Rock Magnetism offering graduate students in the geosciences with instruction in rock magnetism theory and hands-on lab training.

Automating X-ray Fluorescence Analysis for Rapid Astrobiology Surveys.

PubMed

Thompson, David R; Flannery, David T; Lanka, Ravi; Allwood, Abigail C; Bue, Brian D; Clark, Benton C; Elam, W Timothy; Estlin, Tara A; Hodyss, Robert P; Hurowitz, Joel A; Liu, Yang; Wade, Lawrence A

2015-11-01

A new generation of planetary rover instruments, such as PIXL (Planetary Instrument for X-ray Lithochemistry) and SHERLOC (Scanning Habitable Environments with Raman Luminescence for Organics and Chemicals) selected for the Mars 2020 mission rover payload, aim to map mineralogical and elemental composition in situ at microscopic scales. These instruments will produce large spectral cubes with thousands of channels acquired over thousands of spatial locations, a large potential science yield limited mainly by the time required to acquire a measurement after placement. A secondary bottleneck also faces mission planners after downlink; analysts must interpret the complex data products quickly to inform tactical planning for the next command cycle. This study demonstrates operational approaches to overcome these bottlenecks by specialized early-stage science data processing. Onboard, simple real-time systems can perform a basic compositional assessment, recognizing specific features of interest and optimizing sensor integration time to characterize anomalies. On the ground, statistically motivated visualization can make raw uncalibrated data products more interpretable for tactical decision making. Techniques such as manifold dimensionality reduction can help operators comprehend large databases at a glance, identifying trends and anomalies in data. These onboard and ground-side analyses can complement a quantitative interpretation. We evaluate system performance for the case study of PIXL, an X-ray fluorescence spectrometer. Experiments on three representative samples demonstrate improved methods for onboard and ground-side automation and illustrate new astrobiological science capabilities unavailable in previous planetary instruments. Dimensionality reduction-Planetary science-Visualization.

Predicting inpatient violence using an extended version of the Brøset-Violence-Checklist: instrument development and clinical application.

PubMed

Abderhalden, Christoph; Needham, Ian; Dassen, Theo; Halfens, Ruud; Haug, Hans-Joachim; Fischer, Joachim

2006-04-25

Patient aggression is a common problem in acute psychiatric wards and calls for preventive measures. The timely use of preventive measures presupposes a preceded risk assessment. The Norwegian Brøset-Violence-Checklist (BVC) is one of the few instruments suited for short-time prediction of violence of psychiatric inpatients in routine care. Aims of our study were to improve the accuracy of the short-term prediction of violence in acute inpatient settings by combining the Brøset-Violence-Checklist (BVC) with an overall subjective clinical risk-assessment and to test the application of the combined measure in daily practice. We conducted a prospective cohort study with two samples of newly admitted psychiatric patients for instrument development (219 patients) and clinical application (300 patients). Risk of physical attacks was assessed by combining the 6-item BVC and a 6-point score derived from a Visual Analog Scale. Incidents were registered with the Staff Observation of Aggression Scale-Revised SOAS-R. Test accuracy was described as the area under the receiver operating characteristic curve (AUCROC). The AUCROC of the new VAS-complemented BVC-version (BVC-VAS) was 0.95 in and 0.89 in the derivation and validation study respectively. The BVC-VAS is an easy to use and accurate instrument for systematic short-term prediction of violent attacks in acute psychiatric wards. The inclusion of the VAS-derived data did not change the accuracy of the original BVC.

A New Submersible Imaging-in-flow Instrument to Monitor Nano- and Microplankton: Imaging FlowCytobot

NASA Astrophysics Data System (ADS)

Olson, R. J.; Sosik, H. M.; Shalapyonok, A.

2004-12-01

Understanding of how coastal plankton communities are regulated has traditionally been limited by undersampling, but cabled observatories now provide opportunities to deploy submersible sensors that have high power and data transmission requirements. We have developed an in situ instrument to carry out high-resolution, long term monitoring of phytoplankton and microzooplankton in the size range 10 to100 micrometers, to be deployed at cabled research facilities such as the Martha's Vineyard Coastal Observatory (MVCO). The new instrument is designed to complement FlowCytobot, a submersible flow cytometer currently deployed at MVCO that uses fluorescence and light scattering signals from a laser beam to characterize the smallest phytoplankton cells (less than 10 micrometers). Imaging FlowCytobot uses a combination of flow cytometric and video technology to capture images of organisms for identification and to measure chlorophyll fluorescence associated with each image. Images will be classified using neural net software, while the measurements of chlorophyll fluorescence will allow us to discriminate heterotrophic from phototrophic cells. The new instrument, like the original FlowCytobot is autonomous but remotely programmable. It utilizes a computer controlled syringe pump and distribution valve that allows periodic anti-fouling treatment and analysis of standard beads. Samples are analyzed continuously (0.25 to 2.5 ml per min) and data is sent over a fiber optic link to a remote computer for analysis. Preliminary results indicate that we can detect cells as small as 5 micrometers and discriminate several taxa of diatoms and dinoflagellates.

Measuring quality of life in patients with stress urinary incontinence: is the ICIQ-UI-SF adequate?

PubMed

Kurzawa, Zuzanna; Sutherland, Jason M; Crump, Trafford; Liu, Guiping

2018-05-08

The International Consultation on Incontinence Questionnaire Short Form (ICIQ-UI-SF) is a widely used four-item patient-reported outcome (PRO) measure. Evaluations of this instrument are limited, restraining user's confidence in the instrument. This study conducts a comprehensive evaluation of the ICIQ-UI-SF on a sample of urological surgery patients in Canada. One hundred and seventy-seven surgical patients with stress urinary incontinence completed the ICIQ-UI-SF pre-operatively. Methods drawing from confirmatory factor analysis (CFA), measures of reliability, item response theory (IRT), and differential item functioning were applied. Ceiling effects were examined. Ceiling effects were identified. In the CFA, the factor loadings of items one and two differed significantly (p < 0.001) from item three indicating possible multidimensionality. The first two items reflect symptom severity not quality of life. Reliability was moderate as measured by Cronbach's alpha (0.63) and McDonald's coefficient (0.65). The IRT found the instrument does not discriminate between individuals with low incontinence-related quality of life. Due to low/moderate reliability, the ICIQ-UI-SF can be used as a complement to other data or used to report aggregated surgical outcomes among surgical patients. If the primary objective is to measure quality of life, other PROs should be considered.

Ground-based solar astrometric measurements during the PICARD mission

NASA Astrophysics Data System (ADS)

Irbah, A.; Meftah, M.; Corbard, T.; Ikhlef, R.; Morand, F.; Assus, P.; Fodil, M.; Lin, M.; Ducourt, E.; Lesueur, P.; Poiet, G.; Renaud, C.; Rouze, M.

2011-11-01

PICARD is a space mission developed mainly to study the geometry of the Sun. The satellite was launched in June 2010. The PICARD mission has a ground program which is based at the Calern Observatory (Observatoire de la C^ote d'Azur). It will allow recording simultaneous solar images from ground. Astrometric observations of the Sun using ground-based telescopes need however an accurate modelling of optical e®ects induced by atmospheric turbulence. Previous works have revealed a dependence of the Sun radius measurements with the observation conditions (Fried's parameter, atmospheric correlation time(s) ...). The ground instruments consist mainly in SODISM II, replica of the PICARD space instrument and MISOLFA, a generalized daytime seeing monitor. They are complemented by standard sun-photometers and a pyranometer for estimating a global sky quality index. MISOLFA is founded on the observation of Angle-of-Arrival (AA) °uctuations and allows us to analyze atmospheric turbulence optical e®ects on measurements performed by SODISM II. It gives estimations of the coherence parameters characterizing wave-fronts degraded by the atmospheric turbulence (Fried's parameter, size of the isoplanatic patch, the spatial coherence outer scale and atmospheric correlation times). This paper presents an overview of the ground based instruments of PICARD and some results obtained from observations performed at Calern observatory in 2011.

CalWater 2 - Precipitation, Aerosols, and Pacific Atmospheric Rivers Experiment

NASA Astrophysics Data System (ADS)

Spackman, Ryan; Ralph, Marty; Prather, Kim; Cayan, Dan; DeMott, Paul; Dettinger, Mike; Fairall, Chris; Leung, Ruby; Rosenfeld, Daniel; Rutledge, Steven; Waliser, Duane; White, Allen

2014-05-01

Emerging research has identified two phenomena that play key roles in the variability of the water supply and the incidence of extreme precipitation events along the West Coast of the United States. These phenomena include the role of (1) atmospheric rivers (ARs) in delivering much of the precipitation associated with major storms along the U.S. West Coast, and (2) aerosols—from local sources as well as those transported from remote continents—and their modulating effects on western U.S. precipitation. A better understanding of these processes is needed to reduce uncertainties in weather predictions and climate projections of extreme precipitation and its effects, including the provision of beneficial water supply. This presentation summarizes science gaps associated with (1) the evolution and structure of ARs including cloud and precipitation processes and air-sea interaction, and (2) aerosol interaction with ARs and the impact on precipitation, including locally-generated aerosol effects on orographic precipitation along the U.S. West Coast. Observations are proposed for multiple winter seasons as part of a 5-year broad interagency vision referred to as CalWater 2 to address these science gaps (http://esrl.noaa.gov/psd/calwater). In the near term, a science investigation is being planned including a targeted set of aircraft and ship-based measurements and associated evaluation of data in near-shore regions of California and in the eastern Pacific for an intensive observing period between January 2015 and March 2015. DOE's Atmospheric Radiation Measurement (ARM) program and NOAA are coordinating on deployment of airborne and ship-borne facilities for this period in a DOE-sponsored study called ACAPEX (ARM Cloud Aerosol and Precipitation Experiment) to complement CalWater 2. The motivation for this major study is based on findings that have emerged in the last few years from airborne and ground-based studies including CalWater and NOAA's HydroMeterology Testbed (HMT). The proposed observing strategy would build on these advances and employ airborne, ship-, and ground-based assets together with satellite observations to address the scientific objectives. The approach takes advantage of recent investments in new instrumentation, such as the new sophisticated instrumentation developed at University of California San Diego to measure the chemical composition of nucleated aerosols, and also in observing systems, including HMT, the NASA Global Hawk, and relevant satellite and airborne remote sensing observing systems.

Complement-fixing Activity of Fulvic Acid from Shilajit and Other Natural Sources

PubMed Central

Schepetkin, Igor A.; Xie, Gang; Jutila, Mark A.; Quinn, Mark T.

2008-01-01

Shilajit has been used traditionally in folk medicine for treatment of a variety of disorders, including syndromes involving excessive complement activation. Extracts of Shilajit contain significant amounts of fulvic acid (FA), and it has been suggested that FA is responsible for many therapeutic properties of Shilajit. However, little is known regarding physical and chemical properties of Shilajit extracts, and nothing is known about their effects on the complement system. To address this issue, we fractionated extracts of commercial Shilajit using anion exchange and size-exclusion chromatography. One neutral (S-I) and two acidic (S-II and S-III) fractions were isolated, characterized, and compared with standardized FA samples. The most abundant fraction (S-II) was further fractionated into three sub-fractions (S-II-1 to S-II-3). The van Krevelen diagram showed that the Shilajit fractions are products of polysaccharide degradation, and all fractions, except S-II-3, contained type II arabinogalactan. All Shilajit fractions exhibited dose-dependent complement-fixing activity in vitro with high potency. Furthermore, we found a strong correlation between complement-fixing activity and carboxylic group content in the Shilajit fractions and other FA sources. These data provide a molecular basis to explain at least part of the beneficial therapeutic properties of Shilajit and other humic extracts. PMID:19107845

Evaluation of Pasteurella multocida serotype B:2 resistance to immune serum and complement system

PubMed Central

Ataei Kachooei, Saeed; Ranjbar, Mohammad Mehdi; Ataei Kachooei, Saba

2017-01-01

Members of gram-negative bacteria family Pasteurellaceae, include a large number of important economically human and veterinary pathogens. Organisms belonging to the family can colonize in mucosal surfaces of the respiratory, alimentary, genital tracts and cause diseases in various mammals, birds, and reptiles. Hemorrhagic septicemia is an acute disease of cattle and buffaloes in tropical countries caused by Pasteurella multocida serotype B:2. In the present study, the possible bactericidal activity of immune calf sera in the presence and absence of complement system was investigated. The results showed that P. multocida B:2 is highly resistant to positive serum, containing high levels of IgG and IgM obtained from calves after vaccination, and complement activity in normal fresh calf serum. This organism also grew rapidly in the normal fresh calf serum and the mixture of positive serum as well as normal fresh calf serum. As a control test an E. coli strain was subjected to the same experiment and found completely sensitive to the bactericidal activity of complement in calf and guinea pig fresh sera. Results were indicative of the presence of inhibitory mechanism(s) in P. multocida B:2 against bactericidal activity of immune calf serum and complement system. PMID:29085604

Global Instrumental Seismic Catalog: earthquake relocations for 1900-present

NASA Astrophysics Data System (ADS)

Villasenor, A.; Engdahl, E.; Storchak, D. A.; Bondar, I.

2010-12-01

We present the current status of our efforts to produce a set of homogeneous earthquake locations and improved focal depths towards the compilation of a Global Catalog of instrumentally recorded earthquakes that will be complete down to the lowest magnitude threshold possible on a global scale and for the time period considered. This project is currently being carried out under the auspices of GEM (Global Earthquake Model). The resulting earthquake catalog will be a fundamental dataset not only for earthquake risk modeling and assessment on a global scale, but also for a large number of studies such as global and regional seismotectonics; the rupture zones and return time of large, damaging earthquakes; the spatial-temporal pattern of moment release along seismic zones and faults etc. Our current goal is to re-locate all earthquakes with available station arrival data using the following magnitude thresholds: M5.5 for 1964-present, M6.25 for 1918-1963, M7.5 (complemented with significant events in continental regions) for 1900-1917. Phase arrival time data for earthquakes after 1963 are available in digital form from the International Seismological Centre (ISC). For earthquakes in the time period 1918-1963, phase data is obtained by scanning the printed International Seismological Summary (ISS) bulletins and applying optical character recognition routines. For earlier earthquakes we will collect phase data from individual station bulletins. We will illustrate some of the most significant results of this relocation effort, including aftershock distributions for large earthquakes, systematic differences in epicenter and depth with respect to previous location, examples of grossly mislocated events, etc.

A Measure of the Parent-Team Alliance in Youth Residential Psychiatry: The Revised Short Working Alliance Inventory.

PubMed

Lamers, Audri; Delsing, Marc J M H; van Widenfelt, Brigit M; Vermeiren, Robert R J M

The therapeutic alliance between multidisciplinary teams and parents within youth (semi) residential psychiatry is essential for the treatment process and forms a promising process variable for Routine Outcome Monitoring (ROM). No short evaluative instrument, however, is currently available to assess parent-team alliance. In this study, the Working Alliance Inventory-Short Version (WAV-12), a widely used alliance questionnaire, was adjusted to assess parent-team alliance from both a parent and team perspective within a youth residential setting. Psychometric properties, including factor structure and validity of the subscales, were explored. A sample of youth with mainly complex developmental disorders admitted to 11 inpatient and day patient units of a child and adolescent psychiatric institute participated in this study. The case manager involved with the youth and the primary caregiver of 87 youth completed the revised WAV-12 (WAV-12R). The team version of the WAV-12R showed a good fit to the original conceptualized model, and distinguished Bond, Task and Goal scales. For the parents' version an adjusted model with Insight, Bond and combined Task/Goal scales had the best fit. The reliability and validity of the scales were shown to be good. This paper presents preliminary evidence that the parent and treatment team versions of the WAV-12R are psychometrically sound for assessing parent-team alliance within youth (semi) residential psychiatry in the Netherlands. The team and parents' versions of the WAV-12R are recommended instruments to complement outcome measures in ROM.

Exploration of the Habitability of Mars with the SAM Suite Investigation on the 2009 Mars Science Laboratory

NASA Technical Reports Server (NTRS)

Mahaffy, P. R.; Cabane, M.; Webster, C. R.

2008-01-01

The 2009 Mars Science Laboratory (MSL) with a substantially larger payload capability that any other Mars rover, to date, is designed to quantitatively assess a local region on Mars as a potential habitat for present or past life. Its goals are (1) to assess past or present biological potential of a target environment, (2) to characterize geology and geochemistry at the MSL landing site, and (3) to investigate planetary processes that influence habitability. The Sample Analysis at Mars (SAM) Suite, in its final stages of integration and test, enables a sensitive search for organic molecules and chemical and isotopic analysis of martian volatiles. MSL contact and remote surface and subsurface survey Instruments establish context for these measurements and facilitate sample identification and selection. The SAM instruments are a gas chromatograph (GC), a mass spectrometer (MS), and a tunable laser spectrometer (TLS). These together with supporting sample manipulation and gas processing devices are designed to analyze either the atmospheric composition or gases extracted from solid phase samples such as rocks and fines. For example, one of the core SAM experiment sequences heats a small powdered sample of a Mars rock or soil from ambient to -1300 K in a controlled manner while continuously monitoring evolved gases. This is followed by GCMS analysis of released organics. The general chemical survey is complemented by a specific search for molecular classes that may be relevant to life including atmospheric methane and its carbon isotope with the TLS and biomarkers with the GCMS.

Survivability of immunoassay reagents exposed to the space radiation environment on board the ESA BIOPAN-6 platform as a prelude to performing immunoassays on Mars.

PubMed

Derveni, Mariliza; Allen, Marjorie; Sawakuchi, Gabriel O; Yukihara, Eduardo G; Richter, Lutz; Sims, Mark R; Cullen, David C

2013-01-01

The Life Marker Chip (LMC) instrument is an immunoassay-based sensor that will attempt to detect signatures of life in the subsurface of Mars. The molecular reagents at the core of the LMC have no heritage of interplanetary mission use; therefore, the design of such an instrument must take into account a number of risk factors, including the radiation environment that will be encountered during a mission to Mars. To study the effects of space radiation on immunoassay reagents, primarily antibodies, a space study was performed on the European Space Agency's 2007 BIOPAN-6 low-Earth orbit (LEO) space exposure platform to complement a set of ground-based radiation studies. Two antibodies were used in the study, which were lyophilized and packaged in the intended LMC format and loaded into a custom-made sample holder unit that was mounted on the BIOPAN-6 platform. The BIOPAN mission went into LEO for 12 days, after which all samples were recovered and the antibody binding performance was measured via enzyme-linked immunosorbent assays (ELISA). The factors expected to affect antibody performance were the physical conditions of a space mission and the exposure to space conditions, primarily the radiation environment in LEO. Both antibodies survived inactivation by these factors, as concluded from the comparison between the flight samples and a number of shipping and storage controls. This work, in combination with the ground-based radiation tests on representative LMC antibodies, has helped to reduce the risk of using antibodies in a planetary exploration mission context.

Development and Calibration of a Field-Deployable Microphone Phased Array for Propulsion and Airframe Noise Flyover Measurements

NASA Technical Reports Server (NTRS)

Humphreys, William M., Jr.; Lockard, David P.; Khorrami, Mehdi R.; Culliton, William G.; McSwain, Robert G.; Ravetta, Patricio A.; Johns, Zachary

2016-01-01

A new aeroacoustic measurement capability has been developed consisting of a large channelcount, field-deployable microphone phased array suitable for airframe noise flyover measurements for a range of aircraft types and scales. The array incorporates up to 185 hardened, weather-resistant sensors suitable for outdoor use. A custom 4-mA current loop receiver circuit with temperature compensation was developed to power the sensors over extended cable lengths with minimal degradation of the signal to noise ratio and frequency response. Extensive laboratory calibrations and environmental testing of the sensors were conducted to verify the design's performance specifications. A compact data system combining sensor power, signal conditioning, and digitization was assembled for use with the array. Complementing the data system is a robust analysis system capable of near real-time presentation of beamformed and deconvolved contour plots and integrated spectra obtained from array data acquired during flyover passes. Additional instrumentation systems needed to process the array data were also assembled. These include a commercial weather station and a video monitoring / recording system. A detailed mock-up of the instrumentation suite (phased array, weather station, and data processor) was performed in the NASA Langley Acoustic Development Laboratory to vet the system performance. The first deployment of the system occurred at Finnegan Airfield at Fort A.P. Hill where the array was utilized to measure the vehicle noise from a number of sUAS (small Unmanned Aerial System) aircraft. A unique in-situ calibration method for the array microphones using a hovering aerial sound source was attempted for the first time during the deployment.

The STEREO Mission: A New Approach to Space Weather Research

NASA Technical Reports Server (NTRS)

Kaiser, michael L.

2006-01-01

With the launch of the twin STEREO spacecraft in July 2006, a new capability will exist for both real-time space weather predictions and for advances in space weather research. Whereas previous spacecraft monitors of the sun such as ACE and SOH0 have been essentially on the sun-Earth line, the STEREO spacecraft will be in 1 AU orbits around the sun on either side of Earth and will be viewing the solar activity from distinctly different vantage points. As seen from the sun, the two spacecraft will separate at a rate of 45 degrees per year, with Earth bisecting the angle. The instrument complement on the two spacecraft will consist of a package of optical instruments capable of imaging the sun in the visible and ultraviolet from essentially the surface to 1 AU and beyond, a radio burst receiver capable of tracking solar eruptive events from an altitude of 2-3 Rs to 1 AU, and a comprehensive set of fields and particles instruments capable of measuring in situ solar events such as interplanetary magnetic clouds. In addition to normal daily recorded data transmissions, each spacecraft is equipped with a real-time beacon that will provide 1 to 5 minute snapshots or averages of the data from the various instruments. This beacon data will be received by NOAA and NASA tracking stations and then relayed to the STEREO Science Center located at Goddard Space Flight Center in Maryland where the data will be processed and made available within a goal of 5 minutes of receipt on the ground. With STEREO's instrumentation and unique view geometry, we believe considerable improvement can be made in space weather prediction capability as well as improved understanding of the three dimensional structure of solar transient events.

THOR Field and Wave Processor - FWP

NASA Astrophysics Data System (ADS)

Soucek, Jan; Rothkaehl, Hanna; Balikhin, Michael; Zaslavsky, Arnaud; Nakamura, Rumi; Khotyaintsev, Yuri; Uhlir, Ludek; Lan, Radek; Yearby, Keith; Morawski, Marek; Winkler, Marek

2016-04-01

If selected, Turbulence Heating ObserveR (THOR) will become the first mission ever flown in space dedicated to plasma turbulence. The Fields and Waves Processor (FWP) is an integrated electronics unit for all electromagnetic field measurements performed by THOR. FWP will interface with all fields sensors: electric field antennas of the EFI instrument, the MAG fluxgate magnetometer and search-coil magnetometer (SCM) and perform data digitization and on-board processing. FWP box will house multiple data acquisition sub-units and signal analyzers all sharing a common power supply and data processing unit and thus a single data and power interface to the spacecraft. Integrating all the electromagnetic field measurements in a single unit will improve the consistency of field measurement and accuracy of time synchronization. The feasibility of making highly sensitive electric and magnetic field measurements in space has been demonstrated by Cluster (among other spacecraft) and THOR instrumentation complemented by a thorough electromagnetic cleanliness program will further improve on this heritage. Taking advantage of the capabilities of modern electronics, FWP will provide simultaneous synchronized waveform and spectral data products at high time resolution from the numerous THOR sensors, taking advantage of the large telemetry bandwidth of THOR. FWP will also implement a plasma a resonance sounder and a digital plasma quasi-thermal noise analyzer designed to provide high cadence measurements of plasma density and temperature complementary to data from particle instruments. FWP will be interfaced with the particle instrument data processing unit (PPU) via a dedicated digital link which will enable performing on board correlation between waves and particles, quantifying the transfer of energy between waves and particles. The FWP instrument shall be designed and built by an international consortium of scientific institutes from Czech Republic, Poland, France, UK, Sweden and Austria.

On the compatibility of Brewer total column ozone measurements in two adjacent valleys (Arosa and Davos) in the Swiss Alps

NASA Astrophysics Data System (ADS)

Stübi, René; Schill, Herbert; Klausen, Jörg; Vuilleumier, Laurent; Gröbner, Julian; Egli, Luca; Ruffieux, Dominique

2017-11-01

The Arosa site is well known in the ozone community for its continuous total ozone column observations that have been recorded since 1926. Originally based on Dobson sun spectrophotometers, the site has been gradually complemented by three automatic Brewer instruments, in operation since 1998. To secure the long-term ozone monitoring in this Alpine region and to benefit from synergies with the World Radiation Center, the feasibility of moving this activity to the nearby site at Davos (aerial distance of 13 km) has been explored. Concerns about a possible rupture of the 90-year-long record has motivated a careful comparison of the two sites, since great attention to the data continuity and quality has always been central to the operations of the observatory at Arosa. To this end, one element of the Arosa Brewer triad has been set up at the Davos site since November 2011 to realize a campaign of parallel measurements and to study the deviations between the three Brewer instruments. The analysis of the coincident measurement shows that the differences between Arosa and Davos remain within the range of the long-term stability of the Brewer instruments. A nonsignificant seasonal cycle is observed, which could possibly be induced by a stray-light bias and the altitude difference between the two sites. These differences are shown to be lower than the short-term variability of the time series and the overall uncertainty from individual Brewer instruments and therefore are not statistically significant. It is therefore concluded that the world's longest time series of the total ozone column obtained at Arosa site could be safely extended and continued with measurements taken from instruments located at the nearby Davos site without introducing a bias to this unique record.

A Technology Development Roadmap for a Near-Term Probe-Class X-ray Astrophysics Mission

NASA Technical Reports Server (NTRS)

Daelemans, Gerard J.; Petre, Robert; Bookbinder, Jay; Ptak, Andrew; Smith, Randall

2013-01-01

This document presents a roadmap, including proposed budget and schedule, for maturing the instrumentation needed for an X-ray astrophysics Probe-class mission. The Physics of the Cosmos (PCOS) Program Office was directed to create this roadmap following the December 2012 NASA Astrophysics Implementation Plan (AIP). Definition of this mission is called for in the AIP, with the possibility of selection in 2015 for a start in 2017. The overall mission capabilities and instrument performance requirements were defined in the 2010 Astronomy and Astrophysics Decadal Survey report, New Worlds, New Horizons in Astronomy and Astrophysics (NWNH), in connection with the highly ranked International X-ray Observatory (IXO). In NWNH, recommendations were provided regarding the size of, and instrumentation needed by, the next large X-ray observatory. Specifically, the key instrumental capability would be an X-ray calorimeter spectrometer at the focus of a large mirror with angular resolution of 10 arc seconds (arcsec) or better. If possible, a grating spectrometer should also be incorporated into the instrument complement. In response to these recommendations, four instrumentation technologies are included in this roadmap. Three of these are critical for an X-ray mission designed to address NWNH questions: segmented X-ray mirrors, transition edge sensor calorimeters, and gratings. Two approaches are described for gratings, which represent the least mature technology and thus most in need of a parallel path for risk reduction. Also, while current CCD detectors would likely meet the mission needs for grating spectrum readout, specific improvements are included as an additional approach for achieving the grating system effective area requirement. The technical steps needed for these technologies to attain technology readiness levels (TRL) of 5 and 6 are described, as well as desirable modest risk reduction steps beyond TRL-6. All of the technology development efforts are currently funded through the NASA Physics of the Cosmos (PCOS) Strategic Astrophysics Technology (SAT) program; some through the end of FY13, others though FY14. These technology needs are those identified as critical for a near-term mission and briefly described in the 2012 NASA X-ray Mission Concepts Study. This Technology Development Roadmap (TDR) provides a more complete description of each, updates the status, and describes the steps to mature them. For each technology, a roadmap is presented for attaining TRL-6 by 2020 at the latest, and 2018 for most. The funding required for each technology to attain TRL-5 and TRL-6 is presented and justified through a description of the steps needing completion. The total funding required for these technologies to reach TRL-6 is relatively modest, and is consistent with the planned PCOS SAT funding over the next several years. The approximate annual cost through 2018 is $8M. The total cost for all technologies to be matured is $62M (including funding already awarded for FY13 and FY14). This can be contrasted to the $180M recommended by NWNH for technology development for IXO, primarily for the maturation of the mirror technology. The technology described in Section 3 of this document is exclusively that needed for a near-term Probe-class mission, to start in 2017, or for a mission that can be recommended by the next Decadal survey committee for an immediate start. It is important to note that there are other critical X-ray instrumentation technologies under development that are less mature than the ones discussed here, but are essential for a major X-ray mission that might start in the late 2020s. These technologies, described briefly in Section 4, are more appropriately funded through the Astronomy and Physics Research and Analysis (APRA) program.

Detection of HLA Antibodies in Organ Transplant Recipients – Triumphs and Challenges of the Solid Phase Bead Assay

PubMed Central

Tait, Brian D.

2016-01-01

This review outlines the development of human leukocyte antigen (HLA) antibody detection assays and their use in organ transplantation in both antibody screening and crossmatching. The development of sensitive solid phase assays such as the enzyme-linked immunosorbent assay technique, and in particular the bead-based technology has revolutionized this field over the last 10–15 years. This revolution however has created a new paradigm in clinical decision making with respect to the detection of low level pretransplant HLA sensitization and its clinical relevance. The relative sensitivities of the assays used are discussed and the relevance of conflicting inter-assay results. Each assay has its advantages and disadvantages and these are discussed. Over the last decade, the bead-based assay utilizing the Luminex® fluorocytometer instrument has become established as the “gold standard” for HLA antibody testing. However, there are still unresolved issues surrounding this technique, such as the presence of denatured HLA molecules on the beads which reveal cryptic epitopes and the issue of appropriate fluorescence cut off values for positivity. The assay has been modified to detect complement binding (CB) in addition to non-complement binding (NCB) HLA antibodies although the clinical relevance of the CB and NCB IgG isotypes is not fully resolved. The increase sensitivity of the Luminex® bead assay over the complement-dependent cytotoxicity crossmatch has permitted the concept of the “virtual crossmatch” whereby the crossmatch is predicted to a high degree of accuracy based on the HLA antibody specificities detected by the solid phase assay. Dialog between clinicians and laboratory staff on an individual patient basis is essential for correct clinical decision making based on HLA antibody results obtained by the various techniques. PMID:28018342

Targeted organ generation using Mixl1-inducible mouse pluripotent stem cells in blastocyst complementation.

PubMed

Kobayashi, Toshihiro; Kato-Itoh, Megumi; Nakauchi, Hiromitsu

2015-01-15

Generation of functional organs from patients' own cells is one of the ultimate goals of regenerative medicine. As a novel approach to creation of organs from pluripotent stem cells (PSCs), we employed blastocyst complementation in organogenesis-disabled animals and successfully generated PSC-derived pancreas and kidneys. Blastocyst complementation, which exploits the capacity of PSCs to participate in forming chimeras, does not, however, exclude contribution of PSCs to the development of tissues-including neural cells or germ cells-other than those specifically targeted by disabling of organogenesis. This fact provokes ethical controversy if human PSCs are to be used. In this study, we demonstrated that forced expression of Mix-like protein 1 (encoded by Mixl1) can be used to guide contribution of mouse embryonic stem cells to endodermal organs after blastocyst injection. We then succeeded in applying this method to generate functional pancreas in pancreatogenesis-disabled Pdx1 knockout mice using a newly developed tetraploid-based organ-complementation method. These findings hold promise for targeted organ generation from patients' own PSCs in livestock animals.

The participation of complement in the parietal cell antigen–antibody reaction in pernicious anaemia and atrophic gastritis

PubMed Central

Jacob, Elizabeth; Glass, G. B. Jerzy

1969-01-01

Indirect evidence suggests that the parietal cell antibody circulating in the serum of pernicious anaemia patients is a complement fixing antibody. In this work, we have presented direct evidence using an immunofluorescent technique, that the antigen–antibody union occurring in the gastric mucosa between this antibody and the parietal cell antigen binds complement (C'). We have further adduced data to indicate that serum C' activity was decreased in more than one-third of our patients with pernicious anaemia and in one-fourth of those with advanced atrophic gastritis. Eighty-five per cent of the patients with lowered serum C' had parietal cell antibody in the serum and some of them also had intrinsic factor antibody. These findings support the concept of the autoimmune mechanism in the development of the gastric atrophic lesion in a proportion of patients with pernicious anaemia and atrophic gastritis. This mechanism includes the participation of complement in the antigen–antibody reaction at the parietal cell level. ImagesFIG. 1FIG. 2 PMID:4905403

Use of eculizumab in crescentic IgA nephropathy: proof of principle and conundrum?

PubMed Central

Ring, Troels; Pedersen, Birgitte Bang; Salkus, Giedrius; Goodship, Timothy H.J.

2015-01-01

IgA nephropathy (IgAN) is characterized by a variable clinical course and multifaceted pathophysiology. There is substantial evidence to suggest that complement activation plays a pivotal role in the pathogenesis of the disease. Therefore, complement inhibition using the humanized anti-C5 monoclonal antibody eculizumab could be a rational treatment. We report here a 16-year-old male with the vasculitic form of IgAN who failed to respond to aggressive conventional therapy including high-dose steroids, cyclophosphamide and plasma exchange and who was treated with four weekly doses of 900 mg eculizumab followed by a single dose of 1200 mg. He responded rapidly to this treatment and has had a stable creatinine around 150 µmol/L (1.67 mg/dL) for >6 months. However, proteinuria was unabated on maximal conventional anti-proteinuric treatment, and a repeat renal biopsy 11 months after presentation revealed severe chronic changes. We believe this case provides proof of principle that complement inhibition may be beneficial in IgAN but also that development of chronicity may be independent of complement. PMID:26413271

Coagulation and complement system in critically ill patients.

PubMed

Helling, H; Stephan, B; Pindur, G

2015-01-01

Activation of coagulation and inflammatory response including the complement system play a major role in the pathogenesis of critical illness. However, only limited data are available addressing the relationship of both pathways and its assessment of a predictive value for the clinical outcome in intense care medicine. Therefore, parameters of the coagulation and complement system were studied in patients with septicaemia and multiple trauma regarded as being exemplary for critical illness. 34 patients (mean age: 51.38 years (±16.57), 15 females, 19 males) were investigated at day 1 of admittance to the intensive care unit (ICU). Leukocytes, complement factors C3a and C5a were significantly (p <  0.0500) higher in sepsis than in trauma, whereas platelet count and plasma fibrinogen were significantly lower in multiple trauma. Activation markers of coagulation were elevated in both groups, however, thrombin-antithrombin-complex was significantly higher in multiple trauma. DIC scores of 5 were not exceeded in any of the two groups. Analysing the influences on mortality (11/34; 32.35% ), which was not different in both groups, non-survivors were significantly older, had significantly higher multiple organ failure (MOF) scores, lactate, abnormal prothrombin times and lower C1-inhibitor activities, even more pronounced in early deaths, than survivors. In septic non-survivors protein C was significantly lower than in trauma. We conclude from these data that activation of the complement system as part of the inflammatory response is a significant mechanism in septicaemia, whereas loss and consumption of blood components including parts of the coagulation and complement system is more characteristic for multiple trauma. Protein C in case of severe reduction might be of special concern for surviving in sepsis. Activation of haemostasis was occurring in both diseases, however, overt DIC was not confirmed in this study to be a leading mechanism in critically ill patients. MOF score, lactate, C1-inhibitor and prothrombin time have been the only statistically significant predictors for lethal outcome suggesting that organ function, microcirculation, haemostasis and inflammatory response are essential elements of the pathomechanism and clinical course of diseases among critically ill patients.

Activation of the lectin pathway of complement in experimental human keratitis with Pseudomonas aeruginosa.

PubMed

Osthoff, Michael; Brown, Karl D; Kong, David C M; Daniell, Mark; Eisen, Damon P

2014-01-01

Pseudomonas aeruginosa (P. aeruginosa) microbial keratitis (MK) is a sight-threatening disease. Previous animal studies have identified an important contribution of the complement system to the clearance of P. aeruginosa infection of the cornea. Mannose-binding lectin (MBL), a pattern recognition receptor of the lectin pathway of complement, has been implicated in the host defense against P. aeruginosa. However, studies addressing the role of the lectin pathway in P. aeruginosa MK are lacking. Hence, we sought to determine the activity of the lectin pathway in human MK caused by P. aeruginosa. Primary human corneal epithelial cells (HCECs) from cadaveric donors were exposed to two different P. aeruginosa strains. Gene expression of interleukin (IL)-6, IL-8, MBL, and other complement proteins was determined by reverse transcription-polymerase chain reaction (RT-PCR) and MBL synthesis by enzyme-linked immunosorbent assay and intracellular flow cytometry. MBL gene expression was not detected in unchallenged HCECs. Exposure of HCECs to P. aeruginosa resulted in rapid induction of the transcriptional expression of MBL, IL-6, and IL-8. In addition, expression of several complement proteins of the classical and lectin pathways, but not the alternative pathway, were upregulated after 5 h of challenge, including MBL-associated serine protease 1. However, MBL protein secretion was not detectable 18 h after challenge with P. aeruginosa. MK due to P. aeruginosa triggers activation of MBL and the lectin pathway of complement. However, the physiologic relevance of this finding is unclear, as corresponding MBL oligomer production was not observed.

On the value of therapeutic interventions targeting the complement system in acute myocardial infarction.

PubMed

Emmens, Reindert W; Wouters, Diana; Zeerleder, Sacha; van Ham, S Marieke; Niessen, Hans W M; Krijnen, Paul A J

2017-04-01

The complement system plays an important role in the inflammatory response subsequent to acute myocardial infarction (AMI). The aim of this study is to create a systematic overview of studies that have investigated therapeutic administration of complement inhibitors in both AMI animal models and human clinical trials. To enable extrapolation of observations from included animal studies toward post-AMI clinical trials, ex vivo studies on isolated hearts and proof-of-principle studies on inhibitor administration before experimental AMI induction were excluded. Positive therapeutic effects in AMI animal models have been described for cobra venom factor, soluble complement receptor 1, C1-esterase inhibitor (C1-inh), FUT-175, C1s-inhibitor, anti-C5, ADC-1004, clusterin, and glycosaminoglycans. Two types of complement inhibitors have been tested in clinical trials, being C1-inh and anti-C5. Pexelizumab (anti-C5) did not result in reproducible beneficial effects for AMI patients. Beneficial effects were reported in AMI patients for C1-inhibitor, albeit in small patient groups. In general, despite the absence of consistent positive effects in clinical trials thus far, the complement system remains a potentially interesting target for therapy in AMI patients. Based on the study designs of previous animal studies and clinical trials, we discuss several issues which require attention in the design of future studies: adjustment of clinical trial design to precise mechanism of action of administered inhibitor, optimizing the duration of therapy, and optimization of time point(s) on which therapeutic effects will be evaluated. Copyright © 2016 Elsevier Inc. All rights reserved.

Complement Protein C1q Modulates Neurite Outgrowth In Vitro and Spinal Cord Axon Regeneration In Vivo

PubMed Central

Peterson, Sheri L.; Nguyen, Hal X.; Mendez, Oscar A.

2015-01-01

Traumatic injury to CNS fiber tracts is accompanied by failure of severed axons to regenerate and results in lifelong functional deficits. The inflammatory response to CNS trauma is mediated by a diverse set of cells and proteins with varied, overlapping, and opposing effects on histological and behavioral recovery. Importantly, the contribution of individual inflammatory complement proteins to spinal cord injury (SCI) pathology is not well understood. Although the presence of complement components increases after SCI in association with axons and myelin, it is unknown whether complement proteins affect axon growth or regeneration. We report a novel role for complement C1q in neurite outgrowth in vitro and axon regrowth after SCI. In culture, C1q increased neurite length on myelin. Protein and molecular assays revealed that C1q interacts directly with myelin associated glycoprotein (MAG) in myelin, resulting in reduced activation of growth inhibitory signaling in neurons. In agreement with a C1q-outgrowth-enhancing mechanism in which C1q binding to MAG reduces MAG signaling to neurons, complement C1q blocked both the growth inhibitory and repulsive turning effects of MAG in vitro. Furthermore, C1q KO mice demonstrated increased sensory axon turning within the spinal cord lesion after SCI with peripheral conditioning injury, consistent with C1q-mediated neutralization of MAG. Finally, we present data that extend the role for C1q in axon growth and guidance to include the sprouting patterns of descending corticospinal tract axons into spinal gray matter after dorsal column transection SCI. PMID:25762679

Activation of the lectin pathway of complement in experimental human keratitis with Pseudomonas aeruginosa

PubMed Central

Osthoff, Michael; Brown, Karl D.; Kong, David C.M.; Daniell, Mark

2014-01-01

Purpose Pseudomonas aeruginosa (P. aeruginosa) microbial keratitis (MK) is a sight-threatening disease. Previous animal studies have identified an important contribution of the complement system to the clearance of P. aeruginosa infection of the cornea. Mannose-binding lectin (MBL), a pattern recognition receptor of the lectin pathway of complement, has been implicated in the host defense against P. aeruginosa. However, studies addressing the role of the lectin pathway in P. aeruginosa MK are lacking. Hence, we sought to determine the activity of the lectin pathway in human MK caused by P. aeruginosa. Methods Primary human corneal epithelial cells (HCECs) from cadaveric donors were exposed to two different P. aeruginosa strains. Gene expression of interleukin (IL)-6, IL-8, MBL, and other complement proteins was determined by reverse transcription-polymerase chain reaction (RT–PCR) and MBL synthesis by enzyme-linked immunosorbent assay and intracellular flow cytometry. Results MBL gene expression was not detected in unchallenged HCECs. Exposure of HCECs to P. aeruginosa resulted in rapid induction of the transcriptional expression of MBL, IL-6, and IL-8. In addition, expression of several complement proteins of the classical and lectin pathways, but not the alternative pathway, were upregulated after 5 h of challenge, including MBL-associated serine protease 1. However, MBL protein secretion was not detectable 18 h after challenge with P. aeruginosa. Conclusions MK due to P. aeruginosa triggers activation of MBL and the lectin pathway of complement. However, the physiologic relevance of this finding is unclear, as corresponding MBL oligomer production was not observed. PMID:24426774

Science Data Center concepts for moderate-sized NASA missions

NASA Technical Reports Server (NTRS)

Price, R.; Han, D.; Pedelty, J.

1991-01-01

The paper describes the approaches taken by the NASA Science Data Operations Center to the concepts for two future NASA moderate-sized missions, the Orbiting Solar Laboratory (OSL) and the Tropical Rainfall Measuring Mission (TRMM). The OSL space science mission will be a free-flying spacecraft with a complement of science instruments, placed in a high-inclination, sun synchronous orbit to allow continuous study of the sun for extended periods. The TRMM is planned to be a free-flying satellite for measuring tropical rainfall and its variations. Both missions will produce 'standard' data products for the benefit of their communities, and both depend upon their own scientific community to provide algorithms for generating the standard data products.

Examining the Role of Patient Experience Surveys in Measuring Health Care Quality

PubMed Central

Elliott, Marc N.; Zaslavsky, Alan M.; Hays, Ron D.; Lehrman, William G.; Rybowski, Lise; Edgman-Levitan, Susan; Cleary, Paul D.

2015-01-01

Patient care experience surveys evaluate the degree to which care is patient-centered. This article reviews the literature on the association between patient experiences and other measures of health care quality. Research indicates that better patient care experiences are associated with higher levels of adherence to recommended prevention and treatment processes, better clinical outcomes, better patient safety within hospitals, and less health care utilization. Patient experience measures that are collected using psychometrically sound instruments, employing recommended sample sizes and adjustment procedures, and implemented according to standard protocols are intrinsically meaningful and are appropriate complements for clinical process and outcome measures in public reporting and pay-for-performance programs. PMID:25027409

A New All Solid State Approach to Gaseous Pollutant Detection

NASA Technical Reports Server (NTRS)

Brown, V.; Tamstorf, K.

1971-01-01

Recent efforts in our laboratories have concentrated on the development of an all solid state gas sensor, by combining solid electrolyte (ion exchange membrane) technology with advanced thin film deposition processes. With the proper bias magnitude and polarity these miniature electro-chemical,cells show remarkable current responses for many common pollution gases. Current activity is now focused on complementing a multiple array (matrix) of these solid state sensors, with a digital electronic scanner device possessing "scan-compare-identify-alarm: capability. This innovative approach to multi-component pollutant gas analysis may indeed be the advanced prototype for the "third generation" class of pollution analysis instrumentation so urgently needed in the decade ahead.

Spatial correlation of auroral zone geomagnetic variations

NASA Astrophysics Data System (ADS)

Jackel, B. J.; Davalos, A.

2016-12-01

Magnetic field perturbations in the auroral zone are produced by a combination of distant ionospheric and local ground induced currents. Spatial and temporal structure of these currents is scientifically interesting and can also have a significant influence on critical infrastructure.Ground-based magnetometer networks are an essential tool for studying these phenomena, with the existing complement of instruments in Canada providing extended local time coverage. In this study we examine the spatial correlation between magnetic field observations over a range of scale lengths. Principal component and canonical correlation analysis are used to quantify relationships between multiple sites. Results could be used to optimize network configurations, validate computational models, and improve methods for empirical interpolation.

Stratospheric Observatory for Infrared Astronomy (SOFIA)

NASA Astrophysics Data System (ADS)

Becklin, Eric E.

1998-08-01

The joint US and German SOFIA project to develop and operate a 2.5 meter IR airborne telescope in a Boeing 747-SP is now in its second year. The Universities Space Research Association, teamed with Raytheon E-Systems and United Airlines, is developing and will operate SOFIA. The 2.5 meter telescope will be designed and built by a consortium of German companies led by MAN. Work on the aircraft and the primary mirror has started. First science flights will begin in 2001 with 20 percent of the observing time assigned to German investigators. The observatory is expected to operate for over 20 years. The sensitivity, characteristics and science instrument complement are discussed.

Guidelines for the aerosol climatic effects special study: An element of the NASA climate research program

NASA Technical Reports Server (NTRS)

1979-01-01

Research to help develop better understanding of the role of aerosols in the Earth's radiative balance is summarized. Natural volcanic injections of aerosols into the stratosphere to understand and model any resultant evidence of climate change are considered. The approach involves: (1) measurements from aircraft, balloon and ground based platforms which complement and enhance the aerosol information derived from satellite data; (2) development of instruments required for some of these measurements; (3) theoretical and laboratory work to aid in interpreting and utilizing space based and in situ data; and (4) preparation for and execution of concentrated observations of stratospheric aerosols following a future large volcanic eruption.

Constructivism and the projective assessment of meaning in Rorschach administration.

PubMed

Raskin, J D

2001-08-01

Constructivist perspectives on the Rorschach are outlined. I discuss ways in which constructivism complements and adds to existing Rorschach methodologies. It is suggested hat the constructivist emphasis on personally and socially constructed meanings is very consistent with many of the ways the Rorschach has been used and resonates with recent emphases on the Rorschach as a representational task. Furthermore, constructivist perspectives on Rorschach also provide an opportunity to supplement Exner's (1993, 1995) efforts to standardize and norm the Rorschach in a way that maintains the instrument's historic and admirable attention to relational elements of assessment and psychotherapy. I present a variety of meaning-based techniques for conducting Rorschach assessment, incorporating both the constructivist and social constructionist perspective.

Genetics Home Reference: Fanconi anemia

MedlinePlus

... D1 Genetic Testing Registry: Fanconi anemia, complementation group D2 Genetic Testing Registry: Fanconi anemia, complementation group E ... ANEMIA, COMPLEMENTATION GROUP D1 FANCONI ANEMIA, COMPLEMENTATION GROUP D2 FANCONI ANEMIA, COMPLEMENTATION GROUP E FANCONI ANEMIA, COMPLEMENTATION ...

Alternative complement pathway activation fragment Bb in early pregnancy as a predictor of preeclampsia

PubMed Central

Lynch, Anne M.; Murphy, James R.; Byers, Tim; Gibbs, Ronald S.; Neville, Margaret C.; Giclas, Patricia C.; Salmon, Jane E.; Holers, V. Michael

2008-01-01

OBJECTIVE Preeclampsia is a multisystem disease classically defined on the basis of hypertension and proteinuria. As shown in animal studies, complement activation is associated with inflammation in the placenta and adverse pregnancy outcomes. The association between complement activation in humans and adverse pregnancy outcomes is unclear. The purpose of this study was to determine whether elevated levels of the activation fragment Bb in early pregnancy are predictive of preeclampsia. STUDY DESIGN This prospective study of 701 women was conducted in Denver, CO. A single plasma sample was obtained from each woman before 20 weeks’ gestation. The cohort was followed up throughout pregnancy for the development of preeclampsia. Analysis included multivariate logistic regression to adjust for established risk factors for preeclampsia. RESULTS Preeclampsia developed in 4.6% of the cohort. Women with elevated Bb (90th or greater percentile) were substantially more likely to develop preeclampsia than women who had levels less than the 90th percentile (unadjusted relative risk [RR], 3.3, 95% confidence interval [CI] 1.6 to 7, P = .0009). Other significant risk factors for preeclampsia included nulliparity (RR, 2.1, 95% CI, 1–4), a high body mass index (P = .006 for trend), and maternal medical (preexisting maternal hypertension, type 1 diabetes, systemic lupus erythematosus) disease (RR, 4.4, 95% CI, 2–10). Significant risk factors among multiparous women included a history of hypertension in a previous pregnancy (RR, 5, 95% CI, 1.6 to 16) and a change of paternity (RR, 5.1, 95% CI, 1.6 to 15). Adjustment for risk factors did not attenuate the association between an elevated Bb and preeclampsia (adjusted odds ratio [OR], 3.8, 95% CI, 1.6 to 9, P = .002) in the cohort. After removing women with plasma obtained before 10 weeks, the adjusted OR of Bb in the top decile for preeclampsia was 6.1 (95% CI 2.2, 17, P = .0005). CONCLUSION The complement activation product Bb in early pregnancy is a biomarker for elevated risk of preeclampsia. This observation suggests that events linked to activation of complement in early pregnancy are associated with the pathogenesis of preeclampsia. PMID:18221926

Present status of aircraft instruments

NASA Technical Reports Server (NTRS)

1932-01-01

This report gives a brief description of the present state of development and of the performance characteristics of instruments included in the following group: speed instruments, altitude instruments, navigation instruments, power-plant instruments, oxygen instruments, instruments for aerial photography, fog-flying instruments, general problems, summary of instrument and research problems. The items considered under performance include sensitivity, scale errors, effects of temperature and pressure, effects of acceleration and vibration, time lag, damping, leaks, elastic defects, and friction.

Managing cognitive difficulties after traumatic brain injury: a review of online resources for families.

PubMed

Poulin, Valérie; Dawson, Deirdre R; Bottari, Carolina; Verreault, Cynthia; Turcotte, Samantha; Jean, Alexandra

2018-03-22

To identify and critically appraise the content, readability, reliability and usability of websites providing information for managing cognitive difficulties in everyday life for the families of adults with moderate to severe traumatic brain injury. Systematic searches on the Internet for relevant websites were conducted using five search engines, and through consultation of the lists of resources published on websites of traumatic brain injury organizations. Two team members assessed eligibility of the websites. To be included, they had to provide information related to management of cognitive difficulties following moderate to severe traumatic brain injury, to be in English or French and available free of charge. Two reviewers evaluated each website according to: (1) its readability using Flesch-Kincaid Grade Level; (2) the quality of its content using a checklist of eight recommendations for managing memory, attention and executive function problems; (3) its usability (e.g., clear design) and reliability (e.g., currency of information) using the Minervation Validation Instrument for Health Care Web Sites. Of the 38 websites included, 10 provide specific tips for families that cover several domains of cognitive function, including memory, attention and executive function. The most frequent recommendations focused on the use of environmental supports for memory problems (n = 33 websites). The readability of information is below the recommended grade 7 for only nine of the websites. All sites show acceptable usability, but their quality is variable in terms of reliability of the information. This review provides useful information for selecting online resources to educate families about the management of cognitive difficulties following moderate to severe traumatic brain injury, as a complement to information and training provided by the rehabilitation team. Implications for rehabilitation This review describes standardized criteria for the evaluation of the content, readability, reliability and usability of websites for family education post-TBI. Given the variability in the content, the readability and the reliability of websites providing information for families about the management of cognitive difficulties post-TBI, careful attention to the selection of appropriate resources is required. Findings from this review may facilitate clinicians' identification of relevant websites to educate families about the management of cognitive difficulties post-TBI, as a complement to other information and training from the rehabilitation team.

Overview of Lidar Contributions to the Atmospheric Carbon and Transport - America (ACT-America) Program

NASA Astrophysics Data System (ADS)

Davis, K. J.; Meadows, B.; Barrick, J. D. W.; Bell, E.; Browell, E. V.; Campbell, J. F.; DiGangi, J. P.; Chen, G.; Dobler, J. T.; Fan, T. F.; Feng, S.; Fried, A.; Kooi, S. A.; Lauvaux, T.; Lin, B.; McGill, M. J.; Miles, N.; Nehrir, A. R.; Obland, M. D.; O'Dell, C.; Pal, S.; Pauly, R.; Sweeney, C.; Yang, M. Y.

2017-12-01

The Atmospheric Carbon and Transport - America (ACT-America) is an Earth Venture Suborbital -2 (EVS-2) mission sponsored by the Earth Science Division of NASA's Science Mission Directorate. A major objective is to enhance our knowledge of the sources/sinks and transport of atmospheric CO2 through the application of remote and in situ airborne measurements of CO2 and other atmospheric properties on spatial and temporal scales not previously available to the science community. ACT-America consists of five campaigns to measure regional carbon and evaluate transport under various meteorological conditions in three regional areas of the Continental United States. Three of the five campaigns, summer 2016, winter 2017, and fall 2017, have taken place. Data have been collected during these campaigns using 2 airborne platforms (NASA Wallops' C-130 and NASA Langley's B-200) with in-situ instruments on both aircraft and three lidar systems on the C-130, along with instrumented towers and coordinated under flights with the Orbiting Carbon Observatory (OCO-2) satellite. The lidar systems include the Harris Corp. Multi-Frequency Fiber Laser Lidar (MFLL), the NASA Langley ASCENDS CarbonHawk Experiment Simulator (ACES) and the Goddard Cloud Physics Laboratory (CPL). The airborne lidars provide unique data that complement the more traditional in situ sensors. Lidar CO2 measurements provide integrated views of spatial variations of partial columns of atmospheric CO2 which can be adjusted to the column of scientific interest by changing flight altitudes. Lidar backscatter data provide detailed views of atmospheric layers, including the atmospheric boundary layer, residual layers, and cloud layers. The combination of these two lidars provide a far more comprehensive view of atmospheric structure and CO2 content than can be achieved with in situ measurements alone. This presentation provides an overview of the application of these three lidar systems toward achieving ACT-America's scientific objectives.

Engineered Fc variant antibodies with enhanced ability to recruit complement and mediate effector functions

PubMed Central

Moore, Gregory L; Chen, Hsing; Karki, Sher

2010-01-01

Engineering the antibody Fc region to enhance the cytotoxic activity of therapeutic antibodies is currently an active area of investigation. The contribution of complement to the mechanism of action of some antibodies that target cancers and pathogens makes a compelling case for its optimization. Here we describe the generation of a series of Fc variants with enhanced ability to recruit complement. Variants enhanced the cytotoxic potency of an anti-CD20 antibody up to 23-fold against tumor cells in CDC assays, and demonstrated a correlated increase in C1q binding affinity. Complementenhancing substitutions combined additively, and in one case synergistically, with substitutions previously engineered for improved binding to Fc gamma receptors. The engineered combinations provided a range of effector function activities, including simultaneously enhanced CDC, ADCC, and phagocytosis. Variants were also effective at boosting the effector function of antibodies targeting the antigens CD40 and CD19, in the former case enhancing CDC over 600-fold, and in the latter case imparting complement-mediated activity onto an IgG1 antibody that was otherwise incapable of it. This work expands the toolkit of modifications for generating monoclonal antibodies with improved therapeutic potential and enables the exploration of optimized synergy between Fc gamma receptors and complement pathways for the destruction of tumors and infectious pathogens. PMID:20150767

Complement-fixing activity of fulvic acid from Shilajit and other natural sources.

PubMed

Schepetkin, Igor A; Xie, Gang; Jutila, Mark A; Quinn, Mark T

2009-03-01

Shilajit has been used traditionally in folk medicine for the treatment of a variety of disorders, including syndromes involving excessive complement activation. Extracts of Shilajit contain significant amounts of fulvic acid (FA), and it has been suggested that FA is responsible for many therapeutic properties of Shilajit. However, little is known regarding the physical and chemical properties of Shilajit extracts, and nothing is known about their effects on the complement system. To address this issue, extracts of commercial Shilajit were fractionated using anion exchange and size-exclusion chromatography. One neutral (S-I) and two acidic (S-II and S-III) fractions were isolated, characterized and compared with standardized FA samples. The most abundant fraction (S-II) was further fractionated into three sub-fractions (S-II-1 to S-II-3). The van Krevelen diagram showed that the Shilajit fractions are the products of polysaccharide degradation, and all fractions, except S-II-3, contained type II arabinogalactan. All Shilajit fractions exhibited dose-dependent complement-fixing activity in vitro with high potency. Furthermore, a strong correlation was found between the complement-fixing activity and carboxylic group content in the Shilajit fractions and other FA sources. These data provide a molecular basis to explain at least part of the beneficial therapeutic properties of Shilajit and other humic extracts. (c) 2008 John Wiley & Sons, Ltd.

Long-distance Dating: In situ geochronology for planetary missions

NASA Astrophysics Data System (ADS)

Cho, Y.; Cohen, B. A.

2016-12-01

Isotopic dating is an essential tool to establish an absolute chronology for geological events. It enables a planet's crystallization history, magmatic evolution, and alteration to be placed into the framework of solar system history. The capability for in situ geochronology will open up the ability for this crucial measurement to be accomplished as part of lander or rover complement. An in situ geochronology package can also complement sample return missions by identifying the most interesting rocks to cache or return to Earth. The capability of flight instruments to conduct in situ geochronology is called out in the NASA Planetary Science Decadal Survey and the NASA Technology Roadmap as needing development to serve the community's needs. Beagle 2 is the only mission launched to date with the explicit aim to perform in situ K-Ar isotopic dating [1], but it failed to communicate and was lost. The first in situ K-Ar date on Mars, using SAM and APXS measurements on the Cumberland mudstone [2], yielded an age of 4.21 ± 0.35 Ga and validated the idea of K-Ar dating on other planets, though the Curiosity method is not purpose-built for dating and requires many assumptions that degrade its precision. To get more precise and meaningful ages, multiple groups are developing dedicated in situ dating instruments [3-7], including the K-Ar Laser Experiment (KArLE) [5]. KArLE ablates a rock sample, measures K using laser-induced breakdown spectroscopy (LIBS), measures liberated Ar using mass spectrometry (MS), and relates the two by measuring the volume of the ablated pit with optical imaging. The KArLE breadboard tested planetary analog samples yielding ages with 25% uncertainty on very young samples (<50Ma) and 10% uncertainties on older samples. [1] Talboys, et al. (2009) Planetary and Space Science 57, 1237-1245, doi:10.1016/j.pss.2009.02.012. [2] Farley, et al. (2014) Science 343, doi:10.1126/science.1247166. [3] Anderson, et al. (2015) Rapid Comm. Mass Spec. 29, 191-204, doi:10.1002/rcm.7095. [4] Solé (2014) Chem. Geo. doi: 10.1016/j.chemgeo.2014.08.027. [5] Cohen, et al. (2014) Geostand. Geoanaly. Res. 38, 421-439, doi:10.1111/j.1751-908X.2014.00319.x. [6] Farley, et al. (2013) Geochim. Cosmochim. Acta 110, 1-12, doi:10.1016/j.gca.2013.02.010. [7] Cho, et al. (2016) Planet. Space Sci. 128, 14-29.

Oil-in-water monitoring using membrane inlet mass spectrometry.

PubMed

Brkić, Boris; France, Neil; Taylor, Stephen

2011-08-15

A membrane inlet mass spectrometry (MIMS) system has been used for detection and analysis of two types of North Sea crude oil. The system was installed on-field on the Flotta Oil Terminal (Orkney, UK). It consisted of a quadrupole mass spectrometer (QMS) connected to the capillary probe with a silicone-based membrane. The produced mass spectra and calibration plots from the MIMS instrument showed the capability to measure levels of individual hydrocarbons within crude oil in seawater. The generated mass spectra from the field tests also showed the ability to distinguish between different types of oil and to determine concentrations of toxic hydrocarbons in oil (e.g., benzene, toluene, and xylene (BTX)). The performance of the instrument at different temperatures of seawater and oil droplet sizes was also investigated. The results showed that the QMS-based MIMS system has a potential to complement existing oil-in-water (OiW) monitors by being able to detect different oil types and specific hydrocarbon concentrations with high accuracy, which are currently not supported in commercially available OiW monitors.

Direct and Sensitive Detection of CWA Simulants by Active Capillary Plasma Ionization Coupled to a Handheld Ion Trap Mass Spectrometer

NASA Astrophysics Data System (ADS)

Wolf, Jan-Christoph; Etter, Raphael; Schaer, Martin; Siegenthaler, Peter; Zenobi, Renato

2016-07-01

An active capillary plasma ionization (ACI) source was coupled to a handheld mass spectrometer (Mini 10.5; Aston Labs, West Lafayette, IN, USA) and applied to the direct gas-phase detection and quantification of chemical warfare agent (CWA) related chemicals. Complementing the discontinuous atmospheric pressure interface (DAPI) of the Mini 10.5 mass spectrometer with an additional membrane pump, a quasi-continuous sample introduction through the ACI source was achieved. Nerve agent simulants (three dialkyl alkylphosphonates, a dialkyl phosporamidate, and the pesticide dichlorvos) were detected at low gas-phase concentrations with limits of detection ranging from 1.0 μg/m3 to 6.3 μg/m3. Our results demonstrate a sensitivity enhancement for portable MS-instrumentation by using an ACI source, enabling direct, quantitative measurements of volatile organic compounds. Due to its high sensitivity, selectivity, low power consumption (<80 W) and weight (<13 kg), this instrumentation has the potential for direct on-site CWA detection as required by military or civil protection.

Are vision-specific quality of life questionnaires important in assessing rehabilitation for patients with hemianopia post stroke?

PubMed

George, Stacey; Hayes, Allison; Chen, Celia; Crotty, Maria

2011-01-01

To explore the relationship between disability and functional measures with vision-specific quality of life (QoL) measures for people with hemianopia and stroke. The Behavioral Inattention Test (BIT) and the Mayo-Portland Adaptability Inventory (MPAI) were compared with scores on 2 vision-specific QoL measures, the National Eye Institute Visual Function Questionnaire (NEI VFQ-25) and Veteran Low Vision Visual Function Questionnaire (VA LV VFQ-48). Rehabilitation hospitals in Adelaide, South Australia. Stroke patients (n = 24) with homonymous hemianopia. Most of the BIT and MPAI scores were significantly associated with the NEI VFQ-25 and VA LV VFQ-48 scores. Behavioral test scores of the BIT and the MPAI total score correlated with more aspects of the QoL measures than the other components of the BIT and the MPAI. BIT and MPAI measure constructs associated with QoL for people with hemianopia following stroke. Vision-specific QoL questionnaires can complement the functional instruments by identifying the domains of difficulty, based on the instrument's subscale, that can guide rehabilitation therapists to address the person's deficit.

ExoMars Entry, Descent, and Landing Science

NASA Astrophysics Data System (ADS)

Karatekin, Özgür; Forget, Francois; Withers, Paul; Colombatti, Giacomo; Aboudan, Alessio; Lewis, Stephen; Ferri, Francesca; Van Hove, Bart; Gerbal, Nicolas

2016-07-01

Schiaparelli, the Entry Demonstrator Module (EDM) of the ESA ExoMars Program will to land on Mars on 19th October 2016. The ExoMars Atmospheric Mars Entry and Landing Investigations and Analysis (AMELIA) team seeks to exploit the Entry Descent and Landing (EDL) engineering measurements of Schiaparelli for scientific investigations of Mars' atmosphere and surface. ExoMars offers a rare opportunity to perform an in situ investigation of the martian environment over a wide altitude range. There has been only 7 successfully landing on the surface of Mars, from the Viking probes in the 1970's to the Mars Science Laboratory (MSL) in 2012. ExoMars EDM is equipped with an instrumented heat shield like MSL. These novel flight sensors complement conventional accelerometer and gyroscope instrumentation, and provide additional information to reconstruct atmospheric conditions with. This abstract outlines general atmospheric reconstruction methodology using complementary set of sensors and in particular the use of surface pressure and radio data. In addition, we discuss the lessons learned from previous EDL and the plans for ExoMars AMELIA data analysis.

ERS-1 - Our new window on the oceans for the 1990s

NASA Astrophysics Data System (ADS)

Llewellyn-Jones, D. T.

1986-05-01

ESA's First Remote Sensing Satellite (ERS-1) due for launch in 1989 will monitor a carefully selected set of geophysical parameters in an effort to describe the state of the sea-surface. The payload instruments of ERS-1, how they make their measurements, and how the data will be dealt with, are described and discussed. The payload consists of three microwave radars: (1) a wind and wave scatterometer, (2) a synthetic aperture radar, and (3) a radar altimeter. These instruments are complemented by an Along Track Scanning Radiometer and a Precise Range and Range-Rate Experiment. The concept of fast delivery data products is an essential element of the ERS-1 system, meaning that the processed ERS-1 data will be distributed to designated points of contact within three hours of being obtained by the spacecraft. It is concluded that these measurements will lead to a better scientific understanding of ocean-atmosphere interactions; moreover, they will pave the way to the application of such data products to a large range of commercial activities.

Terminal Complement Blockade after Hematopoietic Stem Cell Transplantation Is Safe without Meningococcal Vaccination.

PubMed

Jodele, Sonata; Dandoy, Christopher E; Danziger-Isakov, Lara; Myers, Kasiani C; El-Bietar, Javier; Nelson, Adam; Wallace, Gregory; Teusink-Cross, Ashley; Davies, Stella M

2016-07-01

Eculizumab inhibits terminal complement-mediated intravascular hemolysis in patients with paroxysmal nocturnal hemoglobinuria and complement-mediated thrombotic microangiopathy (TMA) in patients with atypical hemolytic uremic syndrome and is now used as a first-line therapy in these diseases. Eculizumab is available only through a restricted program under a Risk Evaluation and Mitigation Strategy (REMS) because of an increased risk of meningococcal infections in persons without adequate functional complement. Administration of meningococcal vaccine is required at least 2 weeks before administering the first dose of eculizumab, and this advice is included in the product label. Eculizumab use for treatment of TMA in hematopoietic stem cell transplantation (HSCT) recipients brings a significant dilemma regarding REMS required meningococcal vaccination. TMA after HSCT usually occurs within the first 100 days after transplantation when patients are severely immunocompromised and are not able to mount a response to vaccines. We evaluated 30 HSCT recipients treated with eculizumab for high-risk TMA without meningococcal vaccine. All patients received antimicrobial prophylaxis adequate for Neisseria meningitides during eculizumab therapy and for 8 weeks after discontinuation of the drug. Median time to TMA diagnosis was 28 days after transplant (range, 13.8 to 48.5). Study subjects received a median of 14 eculizumab doses (range, 2 to 38 doses) for HSCT-associated TMA therapy. There were no incidences of meningococcal infections. The incidences of bacterial and fungal bloodstream infections were similar in patients treated with eculizumab (n = 30) as compared with those with HSCT-associated TMA who did not receive any complement blocking therapy (n = 39). Our data indicate that terminal complement blockade in the early post-transplant period can be performed without meningococcal vaccination while using appropriate antimicrobial prophylaxis until complement function is restored after therapy completion. Copyright © 2016 American Society for Blood and Marrow Transplantation. Published by Elsevier Inc. All rights reserved.

Guinea pig complement potently measures vibriocidal activity of human antibodies in response to cholera vaccines.

PubMed

Kim, Kyoung Whun; Jeong, Soyoung; Ahn, Ki Bum; Yang, Jae Seung; Yun, Cheol-Heui; Han, Seung Hyun

2017-12-01

The vibriocidal assay using guinea pig complement is widely used for the evaluation of immune responses to cholera vaccines in human clinical trials. However, it is unclear why guinea pig complement has been used over human complement in the measurement of vibriocidal activity of human sera and there have not been comparison studies for the use of guinea pig complement over those from other species. Therefore, we comparatively investigated the effects of complements derived from human, guinea pig, rabbit, and sheep on vibriocidal activity. Complements from guinea pig, rabbit, and human showed concentration-dependent vibriocidal activity in the presence of quality control serum antibodies. Of these complements, guinea pig complement was the most sensitive and effective over a wide concentration range. When the vibriocidal activity of complements was measured in the absence of serum antibodies, human, sheep, and guinea pig complements showed vibriocidal activity up to 40-fold, 20-fold, and 1-fold dilution, respectively. For human pre- and post-vaccination sera, the most potent vibriocidal activity was observed when guinea pig complement was used. In addition, the highest fold-increases between pre- and post- vaccinated sera were obtained with guinea pig complement. Furthermore, human complement contained a higher amount of V. cholerae- and its lipopolysaccharide-specific antibodies than guinea pig complement. Collectively, these results suggest that guinea pig complements are suitable for vibriocidal assays due to their high sensitivity and effectiveness to human sera.

Concurrent Pilot Instrument Monitoring in the Automated Multi-Crew Airline Cockpit.

PubMed

Jarvis, Stephen R

2017-12-01

Pilot instrument monitoring has been described as "inadequate," "ineffective," and "insufficient" after multicrew aircraft accidents. Regulators have called for improved instrument monitoring by flight crews, but scientific knowledge in the area is scarce. Research has tended to investigate the monitoring of individual pilots when in the pilot-flying role; very little research has looked at crew monitoring, or that of the "monitoring-pilot" role despite it being half of the apparent problem. Eye-tracking data were collected from 17 properly constituted and current Boeing 737 crews operating in a full motion simulator. Each crew flew four realistic flight segments, with pilots swapping between the pilot-flying and pilot-monitoring roles, with and without the autopilot engaged. Analysis was performed on the 375 maneuvering-segments prior to localizer intercept. Autopilot engagement led to significantly less visual dwell time on the attitude director indicator (mean 212.8-47.8 s for the flying pilot and 58.5-39.8 s for the monitoring-pilot) and an associated increase on the horizontal situation indicator (18-52.5 s and 36.4-50.5 s). The flying-pilots' withdrawal of attention from the primary flight reference and increased attention to the primary navigational reference was paralleled rather than complemented by the monitoring-pilot, suggesting that monitoring vulnerabilities can be duplicated in the flight deck. Therefore it is possible that accident causes identified as "inadequate" or "insufficient" monitoring, are in fact a result of parallel monitoring.Jarvis SR. Concurrent pilot instrument monitoring in the automated multi-crew airline cockpit. Aerosp Med Hum Perform. 2017; 88(12):1100-1106.

Predicting inpatient violence using an extended version of the Brøset-Violence-Checklist: instrument development and clinical application

PubMed Central

Abderhalden, Christoph; Needham, Ian; Dassen, Theo; Halfens, Ruud; Haug, Hans-Joachim; Fischer, Joachim

2006-01-01

Background Patient aggression is a common problem in acute psychiatric wards and calls for preventive measures. The timely use of preventive measures presupposes a preceded risk assessment. The Norwegian Brøset-Violence-Checklist (BVC) is one of the few instruments suited for short-time prediction of violence of psychiatric inpatients in routine care. Aims of our study were to improve the accuracy of the short-term prediction of violence in acute inpatient settings by combining the Brøset-Violence-Checklist (BVC) with an overall subjective clinical risk-assessment and to test the application of the combined measure in daily practice. Method We conducted a prospective cohort study with two samples of newly admitted psychiatric patients for instrument development (219 patients) and clinical application (300 patients). Risk of physical attacks was assessed by combining the 6-item BVC and a 6-point score derived from a Visual Analog Scale. Incidents were registered with the Staff Observation of Aggression Scale-Revised SOAS-R. Test accuracy was described as the area under the receiver operating characteristic curve (AUCROC). Results The AUCROC of the new VAS-complemented BVC-version (BVC-VAS) was 0.95 in and 0.89 in the derivation and validation study respectively. Conclusion The BVC-VAS is an easy to use and accurate instrument for systematic short-term prediction of violent attacks in acute psychiatric wards. The inclusion of the VAS-derived data did not change the accuracy of the original BVC. PMID:16638122

ROLE OF TYROSINE-SULFATED PROTEINS IN RETINAL STRUCTURE AND FUNCTION

PubMed Central

Kanan, Y.; Al-Ubaidi, M.R.

2014-01-01

The extracellular matrix (ECM) plays a significant role in cellular and retinal health. The study of retinal tyrosine-sulfated proteins is an important first step toward understanding the role of ECM in retinal health and diseases. These secreted proteins are members of the retinal ECM. Tyrosine sulfation was shown to be necessary for the development of proper retinal structure and function. The importance of tyrosine sulfation is further demonstrated by the evolutionary presence of tyrosylprotein sulfotransferases, enzymes that catalyze proteins’ tyrosine sulfation, and the compensatory abilities of these enzymes. Research has identified four tyrosine-sulfated retinal proteins: fibulin 2, vitronectin, complement factor H (CFH), and opticin. Vitronectin and CFH regulate the activation of the complement system and are involved in the etiology of some cases of age-related macular degeneration. Analysis of the role of tyrosine sulfation in fibulin function showed that sulfation influences the protein's ability to regulate growth and migration. Although opticin was recently shown to exhibit anti-angiogenic properties, it is not yet determined what role sulfation plays in that function. Future studies focusing on identifying all of the tyrosine-sulfated retinal proteins would be instrumental in determining the impact of sulfation on retinal protein function in retinal homeostasis and diseases. PMID:25819460

Exoplanet Science with TMT

NASA Astrophysics Data System (ADS)

Crossfield, Ian

2014-07-01

TMT will have unparalleled capabilities for characterizing the composition of extrasolar planets and their atmospheres, and for probing the complex interplay between planet formation, evolution, and migration. In this plenary talk I will summarize these science cases and discuss their synergy with other observing facilities. High-resolution imaging with IRIS and PFI/SEIT will study young, hot planets in nearby star-forming regions, complementing JWST and WFIRST/AFTA coronagraphic efforts at larger semimajor axes. The same instruments will flesh out planets detected by radial velocity (RV) by measuring the albedos and bolometric radii of old, cold Jovian planets and a few ~300 K super-Earths. Complementing JWST and HST studies of short-period transiting planets, NIRES and IRMS spectroscopy will reveal atmospheric composition, dynamics, and thermal structure for dozens of hot Jupiters and Neptunes; NIRES will also produce 2D global maps and movies of a few exoplanets and dozens of brown dwarfs. HROS high-dispersion spectroscopy will precisely measure the composition of extrasolar planetesimals in polluted white dwarfs, and RV followup will continue to exploit the legacies of Kepler, K2, TESS, and PLATO to measure the masses, orbits, and bulk compositions of Earth analogues. Most exciting of all, TMT may facilitate the next major step in the study of exobiology by allowing the detection of biosignature gases around the closest habitable transiting planets.

VLF long-range lightning location using the arrival time difference technique (ATD)

NASA Technical Reports Server (NTRS)

Ierkic, H. Mario

1996-01-01

A new network of VLF receiving systems is currently being developed in the USA to support NASA's Tropical Rain Measuring Mission (TRMM). The new network will be deployed in the east coast of the US, including Puerto Rico, and will be operational in late 1995. The system should give affordable, near real-time, accurate lightning locating capabilities at long ranges and with extended coverage. It is based on the Arrival Time Difference (ATD) method of Lee (1986; 1990). The ATD technique is based on the estimation of the time of arrival of sferics detected over an 18 kHz bandwith. The ground system results will be compared and complemented with satellite optical measurements gathered with the already operational Optical Transient Detector (OTD) instrument and in due course with its successor the Lightning Imaging Sensor (LIS). Lightning observations are important to understand atmospheric electrification phenomena, discharge processes, associated phenomena on earth (e.g. whistlers, explosive Spread-F) and other planets. In addition, lightning is a conspicuous indicator of atmospheric activity whose potential is just beginning to be recognized and utilized. On more prosaic grounds, lightning observations are important for protection of life, property and services.

Atmospheric Balloon Swarms for Persistent In-Situ Measurements in Hurricanes

NASA Astrophysics Data System (ADS)

Meneghello, G.; Bewley, T.

2015-12-01

Real-time measurements within hurricanes are essential to improve forecasts, protect property and save lives. Current methods for obtaining in-situ data, including radar and satellite imagery as well as drop-sondes deployed from repeated aircraft flights above or even within the hurricane itself, are costly, dangerous and limited in duration or resolution. We demonstrate how a swarm of inexpensive, buoyancy-controlled, sensor-laden balloons can be deployed from altitude or from sea-level within a hurricane flow field, and coordinated autonomously in an energetically-efficient fashion to persistently and continuously monitor relevant properties (pressure, humidity, temperature, windspeed) of a hurricane for days at a time. Rather than fighting the gale-force winds in the storm, the strong, predictable stratification of these winds is leveraged to disperse the balloons into a favorable, time-evolving distribution and to follow the hurricane track as it moves. Certain target orbits of interest in the hurricane can be continuously sampled by some balloons, while other balloons make continuous sweeps between the eye and the spiral rain bands. We expect the acquired data to complement current measurement methods and to be instrumental in improving the numerical models' forecast skills.

Advances for Studying Clonal Evolution in Cancer

PubMed Central

Raphael, Benjamin J.; Chen, Feng; Wendl, Michael C.

2013-01-01

The “clonal evolution” model of cancer emerged and “evolved” amid ongoing advances in technology, especially in recent years during which next generation sequencing instruments have provided ever higher resolution pictures of the genetic changes in cancer cells and heterogeneity in tumors. It has become increasingly clear that clonal evolution is not a single sequential process, but instead frequently involves simultaneous evolution of multiple subclones that co-exist because they are of similar fitness or are spatially separated. Co-evolution of subclones also occurs when they complement each other’s survival advantages. Recent studies have also shown that clonal evolution is highly heterogeneous: different individual tumors of the same type may undergo very different paths of clonal evolution. New methodological advancements, including deep digital sequencing of a mixed tumor population, single cell sequencing, and the development of more sophisticated computational tools, will continue to shape and reshape the models of clonal evolution. In turn, these will provide both an improved framework for the understanding of cancer progression and a guide for treatment strategies aimed at the elimination of all, rather than just some, of the cancer cells within a patient. PMID:23353056

Advances for studying clonal evolution in cancer.

PubMed

Ding, Li; Raphael, Benjamin J; Chen, Feng; Wendl, Michael C

2013-11-01

The "clonal evolution" model of cancer emerged and "evolved" amid ongoing advances in technology, especially in recent years during which next generation sequencing instruments have provided ever higher resolution pictures of the genetic changes in cancer cells and heterogeneity in tumors. It has become increasingly clear that clonal evolution is not a single sequential process, but instead frequently involves simultaneous evolution of multiple subclones that co-exist because they are of similar fitness or are spatially separated. Co-evolution of subclones also occurs when they complement each other's survival advantages. Recent studies have also shown that clonal evolution is highly heterogeneous: different individual tumors of the same type may undergo very different paths of clonal evolution. New methodological advancements, including deep digital sequencing of a mixed tumor population, single cell sequencing, and the development of more sophisticated computational tools, will continue to shape and reshape the models of clonal evolution. In turn, these will provide both an improved framework for the understanding of cancer progression and a guide for treatment strategies aimed at the elimination of all, rather than just some, of the cancer cells within a patient. Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

Autonomous Modelling of X-ray Spectra Using Robust Global Optimization Methods

NASA Astrophysics Data System (ADS)

Rogers, Adam; Safi-Harb, Samar; Fiege, Jason

2015-08-01

The standard approach to model fitting in X-ray astronomy is by means of local optimization methods. However, these local optimizers suffer from a number of problems, such as a tendency for the fit parameters to become trapped in local minima, and can require an involved process of detailed user intervention to guide them through the optimization process. In this work we introduce a general GUI-driven global optimization method for fitting models to X-ray data, written in MATLAB, which searches for optimal models with minimal user interaction. We directly interface with the commonly used XSPEC libraries to access the full complement of pre-existing spectral models that describe a wide range of physics appropriate for modelling astrophysical sources, including supernova remnants and compact objects. Our algorithm is powered by the Ferret genetic algorithm and Locust particle swarm optimizer from the Qubist Global Optimization Toolbox, which are robust at finding families of solutions and identifying degeneracies. This technique will be particularly instrumental for multi-parameter models and high-fidelity data. In this presentation, we provide details of the code and use our techniques to analyze X-ray data obtained from a variety of astrophysical sources.

Earth Observations from the International Space Station: Benefits for Humanity

NASA Technical Reports Server (NTRS)

Stefanov, William L.

2015-01-01

The International Space Station (ISS) is a unique terrestrial remote sensing platform for observation of the Earth's land surface, oceans, and atmosphere. Unlike automated remote-sensing platforms it has a human crew; is equipped with both internal and externally-mounted active and passive remote sensing instruments; and has an inclined, low-Earth orbit that provides variable views and lighting (day and night) over 95 percent of the inhabited surface of the Earth. As such, it provides a useful complement to autonomous, sun-synchronous sensor systems in higher altitude polar orbits. Beginning in May 2012, NASA ISS sensor systems have been available to respond to requests for data through the International Charter, Space and Major Disasters, also known as the "International Disaster Charter" or IDC. Data from digital handheld cameras, multispectral, and hyperspectral imaging systems has been acquired in response to IDC activations and delivered to requesting agencies through the United States Geological Survey. The characteristics of the ISS for Earth observation will be presented, including past, current, and planned NASA, International Partner, and commercial remote sensing systems. The role and capabilities of the ISS for humanitarian benefit, specifically collection of remotely sensed disaster response data, will be discussed.

Use of multiple sensor technologies for quality control of in situ biogeochemical measurements: A SeaCycler case study

NASA Astrophysics Data System (ADS)

Atamanchuk, Dariia; Koelling, Jannes; Lai, Jeremy; Send, Uwe; Wallace, Douglas

2017-04-01

Over the last two decades observing capacity for the global ocean has increased dramatically. Emerging sensor technologies for dissolved gases, nutrients and bio-optical properties in seawater are allowing extension of in situ observations beyond the traditionally measured salinity, temperature and pressure (CTD). However the effort to extend observations using autonomous instruments and platforms carries the risk of losing the level of data quality achievable through conventional water sampling techniques. We will present results from a case study with the SeaCycler profiling winch focusing on quality control of the in-situ measurements. A total of 13 sensors were deployed from May 2016 to early 2017 on SeaCycler's profiling sensor float, including CTD, dissolved oxygen (O2, 3 sensors), carbon dioxide (pCO2, 2 sensors), nutrients, velocity sensors, fluorometer, transmissometer, single channel PAR sensor, and others. We will highlight how multiple measurement technologies (e.g. for O2 and CO2) complement each other and result in a high quality data product. We will also present an initial assessment of the bio-optical data, their implications for seasonal phytoplankton dynamics and comparisons to climatologies and ocean-color data products obtained from the MODIS satellite.

Utilization of Airborne and in Situ Data Obtained in SGP99, SMEX02, CLASIC and SMAPVEX08 Field Campaigns for SMAP Soil Moisture Algorithm Development and Validation

NASA Technical Reports Server (NTRS)

Colliander, Andreas; Chan, Steven; Yueh, Simon; Cosh, Michael; Bindlish, Rajat; Jackson, Tom; Njoku, Eni

2010-01-01

Field experiment data sets that include coincident remote sensing measurements and in situ sampling will be valuable in the development and validation of the soil moisture algorithms of the NASA's future SMAP (Soil Moisture Active and Passive) mission. This paper presents an overview of the field experiment data collected from SGP99, SMEX02, CLASIC and SMAPVEX08 campaigns. Common in these campaigns were observations of the airborne PALS (Passive and Active L- and S-band) instrument, which was developed to acquire radar and radiometer measurements at low frequencies. The combined set of the PALS measurements and ground truth obtained from all these campaigns was under study. The investigation shows that the data set contains a range of soil moisture values collected under a limited number of conditions. The quality of both PALS and ground truth data meets the needs of the SMAP algorithm development and validation. The data set has already made significant impact on the science behind SMAP mission. The areas where complementing of the data would be most beneficial are also discussed.

Integrating Existing Simulation Components into a Cohesive Simulation System

NASA Technical Reports Server (NTRS)

McLaughlin, Brian J.; Barrett, Larry K.

2012-01-01

A tradition of leveraging the re-use of components to help manage costs has evolved in the development of complex system. This tradition continues on in the Joint Polar Satellite System (JPSS) Program with the cloning of the Suomi National Polar-orbiting Partnership (NPP) satellite for the JPSS-1 mission, including the instrument complement. One benefit of re-use on a mission is the availability of existing simulation assets from the systems that were previously built. An issue arises in the continual shift of technology over a long mission, or multi-mission, lifecycle. As the missions mature, the requirements for the observatory simulations evolve. The challenge in this environment becomes re-using the existing components in that ever-changing landscape. To meet this challenge, the system must: establish an operational architecture that minimizes impacts on the implementation of individual components, consolidate the satisfaction of new high-impact requirements into system-level infrastructure, and build in a long-term view of system adaptation that spans the full lifecycle of the simulation system. The Flight Vehicle Test Suite (FVTS) within the JPSS Program is defining and executing this approach to ensure a robust simulation capability for the JPSS multi-mission environment

Spatial and Seasonal Variability of Temperature in CO2 Emission from Mars' Mesosphere

NASA Astrophysics Data System (ADS)

Livengood, Timothy A.; Kostiuk, Theodor; Hewagama, Tilak; Kolasinski, John R.; Henning, Wade; Fast, Kelly Elizabeth; Sonnabend, Guido; Sornig, Manuela

2017-10-01

We have observed non-local thermodynamic equilibrium (non-LTE) emission of carbon dioxide that probes Mars’ mesosphere in 2001, 2003, 2007, 2012, 2014, and 2016. These measurements were conducted at 10.6 μm wavelength using the Goddard Space Flight Center Heterodyne Instrument for Planetary Winds and Composition (HIPWAC) from the NASA Infrared Telescope Facility (IRTF) at resolving power (1-33)×106. The Maxwellian broadening of the emission line can be measured at this resolution, providing a direct determination of temperature in the mesosphere. The nonLTE line appears as a narrow emission core within a broad absorption formed by tropospheric CO2, which provides temperature information reaching down to the martian surface, while the mesospheric line probes temperature at about 60-80 km altitude. We will report on the spatial distribution of temperature and emission line strength with local solar time on Mars, with latitude, as well as long-term variability including seasonal effects that modify the overall thermal structure of the atmosphere. These remote measurements complement results from orbital spacecraft through access to a broad range of local solar time on each occasion.This work has been supported by the NASA Planetary Astronomy and Solar Systems Observations Programs

Polymeric Materials

DTIC Science & Technology

2009-06-01

funding have been established, including membership at the BNLS X27C Beam Line, two visiting ASEE Summer faculty, two EOARD programs, two CINT...complement these efforts, collaborative programs with external funding have been established, including membership at the BNLS X27C Beam Line, one visiting

Complement inhibitors for age-related macular degeneration.

PubMed

Williams, Michael A; McKay, Gareth J; Chakravarthy, Usha

2014-01-15

Given the relatively high prevalence of age-related macular degeneration (AMD) and the increased incidence of AMD as populations age, the results of trials of novel treatments are awaited with much anticipation. The complement cascade describes a series of proteolytic reactions occurring throughout the body that generate proteins with a variety of roles including the initiation and promotion of immune reactions against foreign materials or micro-organisms. The complement cascade is normally tightly regulated, but much evidence implicates complement overactivity in AMD and so it is a logical therapeutic target in the treatment of AMD. To assess the effects and safety of complement inhibitors in the prevention or treatment of advanced AMD. We searched CENTRAL (which contains the Cochrane Eyes and Vision Group Trials Register) (The Cochrane Library 2013, Issue 11), Ovid MEDLINE, Ovid MEDLINE In-Process and Other Non-Indexed Citations, Ovid MEDLINE Daily, Ovid OLDMEDLINE (January 1946 to November 2013), EMBASE (January 1980 to November 2013), Allied and Complementary Medicine Database (AMED) (January 1985 to November 2013), Latin American and Caribbean Literature on Health Sciences (LILACS) (January 1982 to November 2013), OpenGrey (System for Information on Grey Literature in Europe) (www.opengrey.eu/), Web of Science Conference Proceedings Citation Index - Science (CPCI-S) (January 1990 to November 2013), the metaRegister of Controlled Trials (mRCT) (www.controlled-trials.com), ClinicalTrials.gov (www.clinicaltrials.gov) and the WHO International Clinical Trials Registry Platform (ICTRP) (www.who.int/ictrp/search/en). We did not use any date or language restrictions in the electronic searches for trials. We last searched the electronic databases on 21 November 2013. We also performed handsearching of proceedings, from 2012 onwards, of meetings and conferences of specific professional organisations. We planned to include randomised controlled trials (RCTs) with parallel treatment groups which investigated either the prevention or treatment of advanced AMD by inhibition of the complement cascade. Two authors (MW and GMcK) independently evaluated all the titles and abstracts resulting from the searches. We contacted companies running clinical trials which had not yet reported results to request information. Since no trials met our inclusion criteria, we undertook no assessment of quality or meta-analysis. We identified and screened 317 references but there were no published RCTs that met the inclusion criteria. We identified two ongoing studies: one phase I study and one phase II study. There is insufficient information at present to generate evidence-based recommendations on the potential safety and efficacy of complement inhibitors for prevention or treatment of AMD. However we anticipate the results of ongoing trials.

Transcultural validation of the ALS-CBS Cognitive Section for the Brazilian population.

PubMed

Branco, Lucas M T; Zanao, Tamires; De Rezende, Thiago J; Casseb, Raphael F; Balthazar, Marcio F; Woolley, Susan C; França, Marcondes C

2017-02-01

Cognitive decline (CD) is common but often under-recognized in ALS due to the scarcity of adequate cognitive screening methods. In this scenario, the Amyotrophic Lateral Sclerosis Cognitive Behavioural Screen (ALS-CBS) is the most investigated instrument and presents high sensitivity to identify CD. Currently, there are no validated cognitive screening tools for ALS patients in the Brazilian population and little is known about the frequency of ALS related CD in the country. We assessed the accuracy of the Brazilian Portuguese version of ALS-CBS Cognitive Section (ALS-CBS-Br) for classifying the cognitive status of Brazilian patients compared to a standard neuropsychological battery, and estimated the prevalence of CD in the Brazilian ALS population. Among 73 initially recruited ALS patients, 49 were included. Twenty-four patients were excluded due to severe motor disability, FTD diagnosis or non-acceptance. Ten healthy controls were also included. Ten ALS patients (20%) were diagnosed with executive dysfunction (ALSci) based on the battery results. ALS-CBS-Br scores were significantly lower in the ALSci group (p < 0.001). The scale accuracy in detecting executive dysfunction was 0.906. Optimal cut-off score was 10/20 (specificity 0.872 and sensitivity 0.900). In conclusion, the ALS-CBS-Br may facilitate the recognition of CD in routine clinical care and complement future studies in our population.

Goodard Space Flight Center/Wallops Flight Facility airborne geoscience support capability

NASA Technical Reports Server (NTRS)

Navarro, Roger L.

1991-01-01

Goddard Space Flight Center's Wallops Facility (GSFC/WFF), operates six aircraft which are used as airborne geoscience platforms. The aircraft complement consists of two UH-1B helicopters, one twin engine Skyvan, one twin jet T-39, and two four engine turboprop aircraft (P-3 and Electra) offering the research community a wide range of payload, altitude, speed, and range capabilities. WFF's support to a principal investigator include mission planning of all supporting elements, installation of equipment on the aircraft, fabrication of brackets, and adapters as required to adapt payloads to the aircraft, and planning of mission profiles to meet science objectives. The flight regime includes local, regional, and global missions. The WFF aircraft serve scientists at GSFC, other NASA centers, other government agencies, and universities. The WFF mode of operation features the walk on method of conducting research projects. The principal investigator requests aircraft support by letter to WFF and after approval is granted, works with the assigned mission manager to plan all phases of project support. The instrumentation is installed in WFF electronics racks, mounted on the aircraft, the missions are flown, and the equipment is removed when the scientific objectives are met. The principal investigator reimburses WFF for each flight hours, any overtime and travel expenses generated by the project, and for other mission-related expenses such as aircraft support services required at deployment bases.

Schur Complement Inequalities for Covariance Matrices and Monogamy of Quantum Correlations

NASA Astrophysics Data System (ADS)

Lami, Ludovico; Hirche, Christoph; Adesso, Gerardo; Winter, Andreas

2016-11-01

We derive fundamental constraints for the Schur complement of positive matrices, which provide an operator strengthening to recently established information inequalities for quantum covariance matrices, including strong subadditivity. This allows us to prove general results on the monogamy of entanglement and steering quantifiers in continuous variable systems with an arbitrary number of modes per party. A powerful hierarchical relation for correlation measures based on the log-determinant of covariance matrices is further established for all Gaussian states, which has no counterpart among quantities based on the conventional von Neumann entropy.

Schur Complement Inequalities for Covariance Matrices and Monogamy of Quantum Correlations.

PubMed

Lami, Ludovico; Hirche, Christoph; Adesso, Gerardo; Winter, Andreas

2016-11-25

We derive fundamental constraints for the Schur complement of positive matrices, which provide an operator strengthening to recently established information inequalities for quantum covariance matrices, including strong subadditivity. This allows us to prove general results on the monogamy of entanglement and steering quantifiers in continuous variable systems with an arbitrary number of modes per party. A powerful hierarchical relation for correlation measures based on the log-determinant of covariance matrices is further established for all Gaussian states, which has no counterpart among quantities based on the conventional von Neumann entropy.

Fast Plasma Instrument for MMS: Data Compression Simulation Results

NASA Astrophysics Data System (ADS)

Barrie, A.; Adrian, M. L.; Yeh, P.; Winkert, G.; Lobell, J.; Vinas, A. F.; Simpson, D. G.

2009-12-01

Magnetospheric Multiscale (MMS) mission will study small-scale reconnection structures and their rapid motions from closely spaced platforms using instruments capable of high angular, energy, and time resolution measurements. To meet these requirements, the Fast Plasma Instrument (FPI) consists of eight (8) identical half top-hat electron sensors and eight (8) identical ion sensors and an Instrument Data Processing Unit (IDPU). The sensors (electron or ion) are grouped into pairs whose 6° x 180° fields-of-view (FOV) are set 90° apart. Each sensor is equipped with electrostatic aperture steering to allow the sensor to scan a 45° x 180° fan about the its nominal viewing (0° deflection) direction. Each pair of sensors, known as the Dual Electron Spectrometer (DES) and the Dual Ion Spectrometer (DIS), occupies a quadrant on the MMS spacecraft and the combination of the eight electron/ion sensors, employing aperture steering, image the full-sky every 30-ms (electrons) and 150-ms (ions), respectively. To probe the diffusion regions of reconnection, the highest temporal/spatial resolution mode of FPI results in the DES complement of a given spacecraft generating 6.5-Mb s-1 of electron data while the DIS generates 1.1-Mb s-1 of ion data yielding an FPI total data rate of 6.6-Mb s-1. The FPI electron/ion data is collected by the IDPU then transmitted to the Central Data Instrument Processor (CIDP) on the spacecraft for science interest ranking. Only data sequences that contain the greatest amount of temporal/spatial structure will be intelligently down-linked by the spacecraft. Currently, the FPI data rate allocation to the CIDP is 1.5-Mb s-1. Consequently, the FPI-IDPU must employ data/image compression to meet this CIDP telemetry allocation. Here, we present updated simulations of the CCSDS 122.0-B-1 algorithm-based compression of the FPI-DES electron data as well as the FPI-DIS ion data. Compression analysis is based upon a seed of re-processed Cluster/PEACE electron measurements and Cluster/CIS ion measurements. Topics to be discussed include: (i) Review of compression algorithm; (ii) Data quality; (iii) Data formatting/organization; (iv) Compression optimization; (v) Investigation of pseudo-log precompression; and (vi) Analysis of compression effectiveness for burst mode as well as fast survey mode data packets for both electron and ion data We conclude with a presentation of the current base-lined FPI data compression approach.

Mpo - the Bepicolombo Mercury Planetary Orbiter.

NASA Astrophysics Data System (ADS)

Benkhoff, J.

2008-09-01

Introduction: BepiColombo is an interdisciplinary mission to explore the planet Mercury through a partnership between ESA and Japan's Aerospace Exploration Agency (JAXA). From their dedicated orbits two spacecrafts, the Mercury Planetary Orbiter (MPO) and the Mercury Magnetospheric Orbiter (MMO), will be studying the planet and its environment Both orbiter will be launched together on an ARIANE 5. The launch is foreseen for Summer 2014 with arrival in Summer 2020. Solar electric propulsion will be used for the journey to Mercury. In November 2004, the BepiColombo scientific payload has been officially approved. Payload of BepiColombo: The MPO scientific payload comprises eleven instruments/instrument packages; the MMO scientific payload consists of five instruments/instrument packages. Together, the scientific payload of both spacecraft will provide the detailed information necessary to understand Mercury and its magnetospheric environment and to find clues to the origin and evolution of a planet close to its parent star. The MPO will focus on a global characterization of Mercury through the investigation of its interior, surface, exosphere and magnetosphere. In addition, it will be testing Einstein's theory of general relativity. Major effort was put into optimizing the scientific return by defining the payload complement such that individual measurements can be interrelated and complement each other. A detailed overview of the status of BepiColombo will be given with special emphasis on the MPO and its payload complement. BepiColombo factsheet BepiColombo is Europe's first mission to Mercury, the innermost planet of the Solar System, and ESA's first science mission in collaboration with Japan. A satellite 'duo' - consisting of an orbiter for planetary investigation and one for magnetospheric studies - Bepi- Colombo will reach Mercury after a six-year journey towards the inner Solar System, to make the most extensive and detailed study of the planet ever performed so far. BepiColombo will also contribute to the understanding of the history and formation of the inner planets of the Solar System in general, including the Earth. The 'Mercury Planetary Orbiter' (MPO), under ESA's responsibility, will study the surface and the internal composition of the planet at different wavelengths and with different techniques. The Mercury Magnetospheric Orbiter (MMO), under the responsibility of the Japan Aerospace Exploration Agency (ISAS/JAXA), will study the magnetosphere, that is the region of space around the planet that is dominated by its magnetic field. Objectives BepiColombo will study and understand the composition, geophysics, atmosphere, magnetosphere and history of Mercury, the least explored planet in the inner Solar System. In particular, the mission objectives are: • markedly higher than that of all other terrestrial planets, Moon included • to understand if the core of Mercury is liquid or solid, and if the planet is still tectonically active today • to understand why such a small planet possesses an intrinsic magnetic field, while Venus, Mars and the Moon do not have any, and investigate if Mercury's magnetised environment is characterised by features reminiscent of the aurorae, radiation belts and magnetospheric substorms observed at Earth • to understand why spectroscopic observations not reveal the presence of any iron, while this element is supposedly the major constituent of the planet • to investigate if the permanently shadowed craters of the polar regions contain sulphur or water ice • to observe the yet unseen hemisphere of Mercury • to study the production mechanisms of the exosphere and to understand the interaction between planetary magnetic field and the solar wind in the absence of a ionosphere • to obtain new clues about the composition of the primordial solar nebula and about the formation of the solar system • to test general relativity with improved accuracy, taking advantage of the proximity of the Sun Since and considering that the advance Mercury's perihelion was explained in terms of relativistic spacetime curvature. MPO Scientific Instruments BepiColombo Mercury Planetary Orbiter's and Mercury Magnetospheric Orbiter's instruments were selected in November 2004, by ESA and JAXA respectively. The MPO will carry a highly sophisticated suit of eleven scientific instruments, ten of which will be provided by Principal Investigators through national funding by ESA Member States and one from Russia: BepiColombo Laser Altimeter (BELA) will characterise the topography and surface morphology of Mercury. It will also provide a digital terrain model that, compared with the data from the MORE instrument, will allow to obtain information about the internal structure, the geology, the tectonics, and the age of the planet's surface. The objectives of the Italian Spring Accelerometer (ISA) are strongly connected with those of the MORE experiment. Together the experiments can give information on Mercury's interior structure as well as test Einstein's theory of the General Relativity. Mercury Magnetometer (MPO-MAG) will provide measurements that will lead to the detailed description of Mercury's planetary magnetic field and its source, to better understand the origin, evolution and current state of the planetary interior , as well as the interaction between Mercury's magnetosphere with the planet's itself and with the solar wind. Mercury Thermal Infrared Spectrometer (MERTIS) will provide detailed information about the mineralogical composition of Mercury's surface layer with a high spectral resolution, crucial for selecting the valid model for origin and evolution of the planet. Mercury Gamma ray and Neutron Spectrometer (MGNS) will determine the elemental compositions of the surface and subsurface of Mercury, and will determine the regional distribution of volatile depositions on the polar areas which are permanently shadowed from the Sun. Mercury Imaging X-ray Spectrometer (MIXS) will use the `X-ray fluorescence' analysis method to produce a global map of the surface atomic composition at high spatial resolution. This technique has been also used by the D-CIXS instrument on ESA's SMART-1 mission to the Moon. Mercury Orbiter Radio science Experiment (MORE) will help to determine the gravity field of Mercury as well as the size and physical state of its core. It will provide crucial experimental constraints to models of the planet's internal structure and test theories of gravity with unprecedented accuracy. The Probing of Hermean Exosphere by Ultraviolet Spectroscopy (PHEBUS) spectrometer is devoted to the characterisation of Mercury's exosphere composition and dynamics. It will also search for surface ice layers in permanently shadowed regions of high-latitude craters. Search for Exosphere Refilling and Emitted Neutral Abundances (Neutral and ionised particle analyser) ( SERENA) will study the gaseaous interaction between surface, exosphere, magnetosphere and solar wind. Spectrometers and Imagers for MPO Bepi- Colombo Integrated Observatory System (SYMBIO-SYS) will examine (also in stereo and colour) the surface geology, volcanism, global tectonics, surface age and composition, and geophysics. Solar Intensity X-ray Spectrometer (SIXS will perform measurements of X-rays and particles of solar origin at high time resolution and a very wide field of view.

Complement component C3 plays a critical role in protecting the aging retina in a murine model of age-related macular degeneration.

PubMed

Hoh Kam, Jaimie; Lenassi, Eva; Malik, Talat H; Pickering, Matthew C; Jeffery, Glen

2013-08-01

Complement component C3 is the central complement component and a key inflammatory protein activated in age-related macular degeneration (AMD). AMD is associated with genetic variation in complement proteins that results in enhanced activation of C3 through the complement alternative pathway. These include complement factor H (CFH), a negative regulator of C3 activation. Both C3 inhibition and/or CFH augmentation are potential therapeutic strategies in AMD. Herein, we examined retinal integrity in aged (12 months) mice deficient in both factors H and C3 (CFH(-/-).C3(-/-)), CFH alone (CFH(-/-)), or C3 alone (C3(-/-)), and wild-type mice (C57BL/6). Retinal function was assessed by electroretinography, and retinal morphological features were analyzed at light and electron microscope levels. Retinas were also stained for amyloid β (Aβ) deposition, inflammation, and macrophage accumulation. Contrary to expectation, electroretinograms of CFH(-/-).C3(-/-) mice displayed more severely reduced responses than those of other mice. All mutant strains showed significant photoreceptor loss and thickening of Bruch's membrane compared with wild-type C57BL/6, but these changes were greater in CFH(-/-).C3(-/-) mice. CFH(-/-).C3(-/-) mice had significantly more Aβ on Bruch's membrane, fewer macrophages, and high levels of retinal inflammation than the other groups. Our data show that both uncontrolled C3 activation (CFH(-/-)) and complete absence of C3 (CFH(-/-).C3(-/-) and C3(-/-)) negatively affect aged retinas. These findings suggest that strategies that inhibit C3 in AMD may be deleterious. Copyright © 2013 American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved.

Small-molecule factor D inhibitors selectively block the alternative pathway of complement in paroxysmal nocturnal hemoglobinuria and atypical hemolytic uremic syndrome.

PubMed

Yuan, Xuan; Gavriilaki, Eleni; Thanassi, Jane A; Yang, Guangwei; Baines, Andrea C; Podos, Steven D; Huang, Yongqing; Huang, Mingjun; Brodsky, Robert A

2017-03-01

Paroxysmal nocturnal hemoglobinuria and atypical hemolytic uremic syndrome are diseases of excess activation of the alternative pathway of complement that are treated with eculizumab, a humanized monoclonal antibody against the terminal complement component C5. Eculizumab must be administered intravenously, and moreover some patients with paroxysmal nocturnal hemoglobinuria on eculizumab have symptomatic extravascular hemolysis, indicating an unmet need for additional therapeutic approaches. We report the activity of two novel small-molecule inhibitors of the alternative pathway component Factor D using in vitro correlates of both paroxysmal nocturnal hemoglobinuria and atypical hemolytic uremic syndrome. Both compounds bind human Factor D with high affinity and effectively inhibit its proteolytic activity against purified Factor B in complex with C3b. When tested using the traditional Ham test with cells from paroxysmal nocturnal hemoglobinuria patients, the Factor D inhibitors significantly reduced complement-mediated hemolysis at concentrations as low as 0.01 μM. Additionally the compound ACH-4471 significantly decreased C3 fragment deposition on paroxysmal nocturnal hemoglobinuria erythrocytes, indicating a reduced potential relative to eculizumab for extravascular hemolysis. Using the recently described modified Ham test with serum from patients with atypical hemolytic uremic syndrome, the compounds reduced the alternative pathway-mediated killing of PIGA -null reagent cells, thus establishing their potential utility for this disease of alternative pathway of complement dysregulation and validating the modified Ham test as a system for pre-clinical drug development for atypical hemolytic uremic syndrome. Finally, ACH-4471 blocked alternative pathway activity when administered orally to cynomolgus monkeys. In conclusion, the small-molecule Factor D inhibitors show potential as oral therapeutics for human diseases driven by the alternative pathway of complement, including paroxysmal nocturnal hemoglobinuria and atypical hemolytic uremic syndrome. Copyright© Ferrata Storti Foundation.

Complement deposition in autoimmune hemolytic anemia is a footprint for difficult-to-detect IgM autoantibodies

PubMed Central

Meulenbroek, Elisabeth M.; de Haas, Masja; Brouwer, Conny; Folman, Claudia; Zeerleder, Sacha S.; Wouters, Diana

2015-01-01

In autoimmune hemolytic anemia autoantibodies against erythrocytes lead to increased clearance of the erythrocytes, which in turn results in a potentially fatal hemolytic anemia. Depending on whether IgG or IgM antibodies are involved, response to therapy is different. Proper identification of the isotype of the anti-erythrocyte autoantibodies is, therefore, crucial. However, detection of IgM autoantibodies can be challenging. We, therefore, set out to improve the detection of anti-erythrocyte IgM. Direct detection using a flow cytometry-based approach did not yield satisfactory improvements. Next, we analyzed whether the presence of complement C3 on a patient’s erythrocytes could be used for indirect detection of anti-erythrocyte IgM. To this end, we fractionated patients’ sera by size exclusion chromatography and tested which fractions yielded complement deposition on erythrocytes. Strikingly, we found that all patients with C3 on their erythrocytes according to standard diagnostic tests had an IgM anti-erythrocyte component that could activate complement, even if no such autoantibody had been detected with any other test. This also included all tested patients with only IgG and C3 on their erythrocytes, who would previously have been classified as having an IgG-only mediated autoimmune hemolytic anemia. Depleting patients’ sera of either IgG or IgM and testing the remaining complement activation confirmed this result. In conclusion, complement activation in autoimmune hemolytic anemia is mostly IgM-mediated and the presence of covalent C3 on patients’ erythrocytes can be taken as a footprint of the presence of anti-erythrocyte IgM. Based on this finding, we propose a diagnostic workflow that will aid in choosing the optimal treatment strategy. PMID:26354757

Unmasking of complements using proteinase-K in formalin fixed paraffin embedded renal biopsies.

PubMed

Nada, R; Kumar, A; Kumar, V G; Gupta, K L; Joshi, K

2016-01-01

Renal biopsy interpretation requires histopathology, direct immunofluorescence (DIF) and electron microscopy. Formalin-fixed, paraffin-embedded tissue (FFPE) sent for light microscopy can be used for DIF after antigen retrieval. However, complement staining has not been satisfactory. We standardized DIF using proteinase-K for antigen retrieval in FFPE renal biopsies. A pilot study was conducted on known cases of membranous glomerulonephritis (MGN), membranoproliferative type-1 (MPGN-1), immunoglobulin A nephropathy (IgAN), and anti-glomerular basement disease (anti-GBM). Immunofluorescence panel included fluorescein isothiocyanate (FITC) conjugated IgG, IgA, IgM, complements (C3 and C1q), light chains (kappa, lambda) and fibrinogen antibodies. After standardization of the technique, 75 renal biopsies and 43 autopsies cases were stained. Out of 43 autopsy cases, immune-complex mediated glomerulonephritis (GN) was confirmed in 18 cases (Lupus nephritis-11, IgAN-6, MGN-1), complement-mediated dense deposit disease (DDD-1) and monoclonal diseases in 4 cases (amyloidosis-3, cast nephropathy-1). Immune-mediated injury was excluded in 17 cases (focal segmental glomerulosclerosis -3, crescentic GN-6 [pauci-immune-3, anti-GBM-3], thrombotic microangiopathy-5, atherosclerosis-3). Renal biopsies (n-75) where inadequate or no frozen sample was available; this technique classified 52 mesangiocapillary pattern as MPGN type-1-46, DDD-2 and (C3GN-4). Others were diagnosed as IgAN-3, lupus nephritis-2, MGN-4, diffuse proliferative glomerulonephritis (DPGN)-1, Non-IC crescentic GN-1, monoclonal diseases-3. In nine cases, DIF on FFPE tissue could not help in making diagnosis. Proteinase-K enzymatic digestion of FFPE renal biopsies can unmask complements (both C3 and C1q) in immune-complexes mediated and complement-mediated diseases. This method showed good results on autopsy tissues archived for as long as 15 years.

Clinical roundtable monograph: Paroxysmal nocturnal hemoglobinuria: a case-based discussion.

PubMed

Szer, Jeff; Hill, Anita; Weitz, Ilene Ceil

2012-11-01

Paroxysmal nocturnal hemoglobinuria (PNH) is a rare, acquired disorder characterized by chronic intravascular hemolysis as the primary clinical manifestation and morbidities that include anemia, thrombosis, renal impairment, pulmonary hypertension, and bone marrow failure. The prevalence of the PNH clone (from <1-100% PNH granulocytes) is approximately 16 per million, and careful monitoring is required. The average age of onset of the clinical disease is the early 30s, although it can present at all ages. PNH is caused by the acquisition of a somatic mutation of the gene phosphatidylinositol glycan anchor (PIG-A) in a multipotent hematopoietic stem cell (HSC), with clonal expansion of the mutated HSC. The mutation causes a deficiency in the synthesis of glycosylphosphatidylinositol (GPI). In cells derived from normal HSCs, the complement regulatory proteins CD55 and CD59 are anchored to the hematopoietic cell membrane surface via GPI, protecting the cells from complement-mediated lysis. However, in patients with PNH, these 2 proteins, along with numerous other GPI-linked proteins, are absent from the cell surface of red cells, granulocytes, monocytes, and platelets, resulting in complement-mediated intravascular hemolysis and other complications. Lysis of red blood cells is the most obvious manifestation, but as other cell lineages are also affected, this complement-mediated attack contributes to additional complications, such as thrombosis. Eculizumab, a humanized monoclonal antibody against the C5 complement protein, is the only effective drug therapy for PNH patients. The antibody prevents cleavage of the C5 protein by C5 convertase, in turn preventing generation of C5b-9 and release of C5a, thereby protecting from hemolysis of cells lacking the CD59 surface protein and other complications associated with complement activation. Drs. Ilene C. Weitz, Anita Hill, and Jeff Szer discuss 3 recent cases of patients with PNH.

Rituximab fails where eculizumab restores renal function in C3nef-related DDD.

PubMed

Rousset-Rouvière, Caroline; Cailliez, Mathilde; Garaix, Florentine; Bruno, Daniele; Laurent, Daniel; Tsimaratos, Michel

2014-06-01

Dense deposit disease (DDD), a C3 glomerulopathy (C3G), is a rare disease with unfavorable progression towards end-stage kidney disease. The pathogenesis of DDD is due to cytotoxic effects related to acquired or genetic dysregulation of the complement alternative pathway, which is at times accompanied by the production of C3 nephritic factor (C3NeF), an auto-antibody directed against the alternative C3 convertase. Available treatments include plasma exchange, CD20-targeted antibodies, and a terminal complement blockade via the anti-C5 monoclonal antibody eculizumab. We report here the case of an 8-year-old child with C3NeF and refractory DDD who presented with a nephritic syndrome. She tested positive for C3NeF activity; C3 was undetectable. Genetic analyses of the alternative complement pathway were normal. Methylprednisolone pulses and mycophenolate mofetil treatment resulted in complete recovery of renal function and a reduction in proteinuria. Corticosteroids were tapered and then withdrawn. Four months after corticosteroid discontinuation, hematuria and proteinuria recurred, and a renal biopsy confirmed an active DDD with a majority of extracapillary crescents. Despite an increase in immunosuppressive drugs, including methylprednisolone pulses and rituximab therapy, the patient suffered acute renal failure within 3 weeks, requiring dialysis. Eculizumab treatment resulted in a quick and impressive response. Hematuria very quickly resolved, kidney function improved, and no further dialysis was required. The patient received bimonthly eculizumab injections of 600 mg, allowing for normalization of renal function and reduction of proteinuria to <0.5 g per day. Since then, she continues to receive eculizumab. Complement regulation pathway-targeted therapy may be a specific and useful treatment for rapidly progressing DDD prior to the development of glomerulosclerosis. Our data provide evidence supporting the pivotal role of complement alternative pathway abnormalities in C3G with DDD.

Far Infrared All-Sky Survey

NASA Technical Reports Server (NTRS)

Richards, Paul L.

1998-01-01

Precise measurements of the angular power spectrum of the Cosmic Microwave Background (CMB) anisotropy will revolutionize cosmology. These measurements will discriminate between competing cosmological models and, if the standard inflationary scenario is correct, will determine each of the fundamental cosmological parameters with high precision. The astrophysics community has recognized this potential: the orbital experiments MAP and PLANCK, have been approved to measure CMB anisotropy. Balloon-borne experiments can realize much of this potential before these missions are launched. Additionally, properly designed balloon-borne experiments can complement MAP in frequency and angular resolution and can give the first realistic test of the instrumentation proposed for the high frequency instrument on PLANCK. The MAXIMA experiment is part of the MAXIMA/BOOMERANG collaboration which is doing balloon observations of the angular power spectrum of the Cosmic Microwave Background from l = 10 to l = 800. These experiments are designed to use the benefits of both North American and Antarctic long-duration ballooning to full advantage. We have developed several new technologies that together allow the power spectrum to be measured with unprecedented combination of angular resolution, beam throw, sensitivity, sky coverage and control of systematic effects. These technologies are the basis for the high frequency instrument for the PLANCK mission. Our measurements will strongly discriminate between models of the origin and evolution of structure in the universe and, for many models, will determine the value of the basic cosmological parameters to high precision.

Long-range Transport of Aerosol at a Mountain Site in the Western Mediterranean Basin

NASA Astrophysics Data System (ADS)

Roberts, Greg; Corrigan, Craig; Ritchie, John; Pont, Véronique; Claeys, Marine; Sciare, Jean; Dulac, François

2016-04-01

The Mediterranean Region has been identified as sensitive to changes in the hydrological cycle, which could affect the water resources for millions of people by the turn of the century. However, prior to recent observations, most climate models have not accounted for the impacts of aerosol in this region. Past airborne studies have shown that aerosol sources from Europe and Africa are often transported throughout the lower troposphere; yet, because of their complex vertical distribution, it is a challenge to capture the variability and quantify the contribution of these sources to the radiative budget and precipitation processes. The PAEROS ChArMEx Mountain Experiment (PACMEx) complemented the regional activities by collecting aerosol data from atop a mountain on the island of Corsica, France in order to assess boundary layer / free troposphere atmospheric processes. In June/July 2013, PACMEx instruments were deployed at 2000 m.asl near the center of Corsica, France to complement ground-based aerosol observations at 550 m.asl on the northern peninsula, as well as airborne measurements. Comparisons between the peninsula site and the mountain site show similar general trends in aerosol properties; yet, differences in aerosol properties reveal the myriad transport mechanisms over the Mediterranean Basin. Using aerosol physicochemical data coupled with back trajectory analysis, different sources have been identified including Saharan dust transport, residual dust mixed with sea salt, anthropogenic emissions from Western Europe, and a period of biomass burning from Eastern Europe. Each period exhibits distinct signatures in the aerosol related to transport processes above and below the boundary layer. In addition, the total aerosol concentrations at the mountain site revealed a strong diurnal cycling the between the atmospheric boundary layer and the free troposphere, which is typical of mountain-top observations. PACMEx was funded by the National Science Foundation (NSF) as a cooperative collaboration with French research agencies (INSU-MISTRALS, ANR-ADRIMED, and Météo France).

Sources and Transport of Aerosol above the Boundary Layer over the Mediterranean Basin

NASA Astrophysics Data System (ADS)

Roberts, Greg; Corrigan, Craig; Ritchie, John; Pont, Veronique; Claeys, Marine; Sciare, Jean; Mallet, Marc; Dulac, François; Mihalopoulos, Nikos

2015-04-01

The Mediterranean Region has been identified as sensitive to changes in the hydrological cycle, which could affect the water resources for millions of people by the turn of the century. However, prior to recent observations, most climate models have not accounted for the impacts of aerosol in this region. Past airborne studies have shown that aerosol sources from Europe and Africa are often transported throughout the lower troposphere; yet, because of their complex vertical distribution, it is a challenge to capture the variability and quantify the contribution of these sources to the radiative budget and precipitation processes. The PAEROS ChArMEx Mountain Experiment (PACMEx) complemented the regional activities by collecting aerosol data from atop a mountain on the island of Corsica, France in order to assess boundary layer / free troposphere atmospheric processes. In June/July 2013, PACMEx instruments were deployed at 2000 m.asl near the center of Corsica, France to complement ground-based aerosol observations at 550 m.asl on the northern peninsula, as well as airborne measurements. Comparisons between the peninsula site and the mountain site show similar general trends in aerosol properties; yet, differences in aerosol properties reveal the myriad transport mechanisms over the Mediterranean Basin. Using aerosol physicochemical data coupled with back trajectory analysis, different sources have been identified including Saharan dust transport, residual dust mixed with sea salt, anthropogenic emissions from Western Europe, and a period of biomass burning from Eastern Europe. Each period exhibits distinct signatures in the aerosol related to transport processes above and below the boundary layer. In addition, the total aerosol concentrations at the mountain site revealed a strong diurnal cycling the between the atmospheric boundary layer and the free troposphere, which is typical of mountain-top observations. PACMEx was funded by the National Science Foundation (NSF) as a cooperative collaboration with French research agencies (INSU-MISTRALS, ANR-ADRIMED, and Météo France).

Using the Spatial Persistence of Soil Moisture Patterns to Estimate Catchment Soil Moisture in Semi-arid Areas

NASA Astrophysics Data System (ADS)

Willgoose, G. R.

2006-12-01

In humid catchments the spatial distribution of soil water is dominated by subsurface lateral fluxes, which leads to a persistent spatial pattern of soil moisture principally described by the topographic index. In contrast, semi-arid, and dryer, catchments are dominated by vertical fluxes (infiltration and evapotranspiration) and persistent spatial patterns, if they exist, are subtler. In the first part of this presentation the results of a reanalysis of a number of catchment-scale long-term spatially-distributed soil moisture data sets are presented. We concentrate on Tarrawarra and SASMAS, both catchments in Australia that are water-limited for at least part of the year and which have been monitored using a variety of technologies. Using the data from permanently installed instruments (neutron probe and reflectometry) both catchments show persistent patterns at the 1-3 year timescale. This persistent pattern is not evident in the field campaign data where field portable instruments (reflectometry) instruments were used. We argue, based on high-resolution soil moisture semivariograms, that high short-distance variability (100mm scale) means that field portable instrument cannot be replaced at the same location with sufficient accuracy to ensure deterministic repeatability of soil moisture measurements from campaign to campaign. The observed temporal persistence of the spatial pattern can be caused by; (1) permanent features of the landscape (e.g. vegetation, soils), or (2) long term memory in the soil moisture store. We argue that it is permanent in which case it is possible to monitor the soil moisture status of a catchment using a single location measurement (continuous in time) of soil moisture using a permanently installed reflectometry instrument. This instrument will need to be calibrated to the catchment averaged soil moisture but the temporal persistence of the spatial pattern of soil moisture will mean that this calibration will be deterministically stable with time. In the second part of this presentation we will explore aspects of the calibration using data from the SASMAS site using the multiscale spatial resolution data (100m to 10km) provided by permanently installed reflectometry instruments, and how this single site measurement technique may complement satellite data.

An Overview of the Mars Reconnaissance Orbiter (MRO) Science Mission

NASA Technical Reports Server (NTRS)

Zurek, Richard W.; Smrekar, Suzanne E.

2007-01-01

The Mars Reconnaissance Orbiter (MRO) is the latest addition to the suite of missions on or orbiting Mars as part of the NASA Mars Exploration Program. Launched on 12 August 2005, the orbiter successfully entered Mars orbit on 10 March 2006 and finished aerobraking on 30 August 2006. Now in its near-polar, near-circular, low-altitude (approximately 300 km), 3 p.m. orbit, the spacecraft is operating its payload of six scientific instruments throughout a one-Mars-year Primary Science Phase (PSP) of global mapping, regional survey, and targeted observations. Eight scientific investigations were chosen for MRO, two of which use either the spacecraft accelerometers or tracking of the spacecraft telecom signal to acquire data needed for analysis. Six instruments, including three imaging systems, a visible-near infrared spectrometer, a shallow-probing subsurface radar, and a thermal-infrared profiler, were selected to complement and extend the capabilities of current working spacecraft at Mars. Whether observing the atmosphere, surface, or subsurface, the MRO instruments are designed to achieve significantly higher resolution while maintaining coverage comparable to the current best observations. The requirements to return higher-resolution data, to target routinely from a low-altitude orbit, and to operate a complex suite of instruments were major challenges successfully met in the design and build of the spacecraft, as well as by the mission design. Calibration activities during the seven-month cruise to Mars and limited payload operations during a three-day checkout prior to the start of aerobraking demonstrated, where possible, that the spacecraft and payload still had the functions critical to the science mission. Two critical events, the deployment of the SHARAD radar antenna and the opening of the CRISM telescope cover, were successfully accomplished in September 2006. Normal data collection began 7 November 2006 after solar conjunction. As part of its science mission, MRO will also aid identification and characterization of the most promising sites for future landed missions, both in terms of safety and in terms of the scientific potential for future discovery. Ultimately, MRO data will advance our understanding of how Mars has evolved and by which processes that change occurs, all within a framework of identifying the presence, extent, and role of water in shaping the planet s climate over time.

Anticomplementary activity of horse IgG and F(ab')2 antivenoms.

PubMed

Squaiella-Baptistão, Carla Cristina; Marcelino, José Roberto; Ribeiro da Cunha, Luiz Eduardo; Gutiérrez, José María; Tambourgi, Denise V

2014-03-01

Envenomation by poisonous animals is a neglected condition according to the World Health Organization (WHO). Antivenoms are included in the WHO Essential Medicines List. It has been assumed that immunoglobulin G (IgG) antivenoms could activate the complement system through Fc and induce early adverse reactions (EARs). However, data in the literature indicate that F(ab')2 fragments can also activate the complement system. Herein, we show that several batches of IgG and F(ab')2 antivenoms from the Butantan, Vital Brazil, and Clodomiro Picado Institutes activated the complement classical pathway and induced the production of C3a; however, only those antivenoms from Clodomiro Picado generated C5a. Different protein profiles (IgG heavy chain, protein contaminants, and aggregates) were observed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analyses. Our results show that various antivenoms from different producers are able to activate the classical pathway of the complement system and generate anaphylatoxins, and these findings suggest that factors, such as composition, contaminant proteins, and aggregates, may influence the anticomplementary activity of antivenoms in vitro. Therefore, there is a need to further improve antivenom production methods to reduce their anticomplementary activity and potential to cause EARs.

The Semantics of Complementation in English: A Cognitive Semantic Account of Two English Complement Constructions

ERIC Educational Resources Information Center

Smith, Michael B.

2009-01-01

Studies on complementation in English and other languages have traditionally focused on syntactic issues, most notably on the constituent structures of different complement types. As a result, they have neglected the role of meaning in the choice of different complements. This paper investigates the semantics of complementation within the…

KSC-97PC1544

NASA Image and Video Library

1997-10-15

A seven-year journey to the ringed planet Saturn begins with the liftoff of a Titan IVB/Centaur carrying the Cassini orbiter and its attached Huygens probe. Launch occurred at 4:43 a.m. EDT, Oct. 15, from Launch Complex 40 on Cape Canaveral Air Station. After a 2.2-billion mile journey that will include two swingbys of Venus and one of Earth to gain additional velocity, the two-story tall spacecraft will arrive at Saturn in July 2004. The orbiter will circle the planet for four years, its complement of 12 scientific instruments gathering data about Saturn's atmosphere, rings and magnetosphere and conducting closeup observations of the Saturnian moons. Huygens, with a separate suite of six science instruments, will separate from Cassini to fly on a ballistic trajectory toward Titan, the only celestial body besides Earth to have an atmosphere rich in nitrogen. Scientists are eager to study further this chemical similarity in hopes of learning more about the origins of our own planet Earth. Huygens will provide the first direct sampling of Titan's atmospheric chemistry and the first detailed photographs of its surface. The Cassini mission is an international effort involving NASA, the European Space Agency (ESA) and the Italian Space Agency, Agenzia Spaziale Italiana (ASI). The Jet Propulsion Laboratory manages the U.S. contribution to the mission for NASA's Office of Space Science. The major U.S. contractor is Lockheed Martin, which provided the launch vehicle and upper stage, spacecraft propulsion module and radioisotope thermoelectric generators that will provide power for the spacecraft. The Titan IV/Centaur is a U.S. Air Force launch vehicle, and launch operations were managed by the 45th Space Wing

KSC-97PC1546

NASA Image and Video Library

1997-10-15

KENNEDY SPACE CENTER, FLA. -- A seven-year journey to the ringed planet Saturn begins with the liftoff of a Titan IVB/Centaur carrying the Cassini orbiter and its attached Huygens probe. Launch occurred at 4:43 a.m. EDT, Oct. 15, from Launch Complex 40 on Cape Canaveral Air Station. After a 2.2-billion mile journey that will include two swingbys of Venus and one of Earth to gain additional velocity, the two-story tall spacecraft will arrive at Saturn in July 2004. The orbiter will circle the planet for four years, its complement of 12 scientific instruments gathering data about Saturn's atmosphere, rings and magnetosphere and conducting closeup observations of the Saturnian moons. Huygens, with a separate suite of six science instruments, will separate from Cassini to fly on a ballistic trajectory toward Titan, the only celestial body besides Earth to have an atmosphere rich in nitrogen. Scientists are eager to study further this chemical similarity in hopes of learning more about the origins of our own planet Earth. Huygens will provide the first direct sampling of Titan's atmospheric chemistry and the first detailed photographs of its surface. The Cassini mission is an international effort involving NASA, the European Space Agency (ESA) and the Italian Space Agency, Agenzia Spaziale Italiana (ASI). The Jet Propulsion Laboratory manages the U.S. contribution to the mission for NASA's Office of Space Science. The major U.S. contractor is Lockheed Martin, which provided the launch vehicle and upper stage, spacecraft propulsion module and radioisotope thermoelectric generators that will provide power for the spacecraft. The Titan IV/Centaur is a U.S. Air Force launch vehicle, and launch operations were managed by the 45th Space Wing

KSC-cassini

NASA Image and Video Library

1997-10-15

A seven-year journey to the ringed planet Saturn begins with the liftoff of a Titan IVB/Centaur carrying the Cassini orbiter and its attached Huygens probe. Launch occurred at 4:43 a.m. EDT, Oct. 15, from Launch Complex 40 on Cape Canaveral Air Station. After a 2.2-billion mile journey that will include two swingbys of Venus and one of Earth to gain additional velocity, the two-story tall spacecraft will arrive at Saturn in July 2004. The orbiter will circle the planet for four years, its complement of 12 scientific instruments gathering data about Saturn's atmosphere, rings and magnetosphere and conducting closeup observations of the Saturnian moons. Huygens, with a separate suite of six science instruments, will separate from Cassini to fly on a ballistic trajectory toward Titan, the only celestial body besides Earth to have an atmosphere rich in nitrogen. Scientists are eager to study further this chemical similarity in hopes of learning more about the origins of our own planet Earth. Huygens will provide the first direct sampling of Titan's atmospheric chemistry and the first detailed photographs of its surface. The Cassini mission is an international effort involving NASA, the European Space Agency (ESA) and the Italian Space Agency, Agenzia Spaziale Italiana (ASI). The Jet Propulsion Laboratory manages the U.S. contribution to the mission for NASA's Office of Space Science. The major U.S. contractor is Lockheed Martin, which provided the launch vehicle and upper stage, spacecraft propulsion module and radioisotope thermoelectric generators that will provide power for the spacecraft. The Titan IV/Centaur is a U.S. Air Force launch vehicle, and launch operations were managed by the 45th Space Wing

KSC-97PC1547

NASA Image and Video Library

1997-10-15

A seven-year journey to the ringed planet Saturn begins with the liftoff of a Titan IVB/Centaur carrying the Cassini orbiter and its attached Huygens probe. Launch occurred at 4:43 a.m. EDT, Oct. 15, from Launch Complex 40 on Cape Canaveral Air Station. After a 2.2-billion mile journey that will include two swingbys of Venus and one of Earth to gain additional velocity, the two-story tall spacecraft will arrive at Saturn in July 2004. The orbiter will circle the planet for four years, its complement of 12 scientific instruments gathering data about Saturn's atmosphere, rings and magnetosphere and conducting closeup observations of the Saturnian moons. Huygens, with a separate suite of six science instruments, will separate from Cassini to fly on a ballistic trajectory toward Titan, the only celestial body besides Earth to have an atmosphere rich in nitrogen. Scientists are eager to study further this chemical similarity in hopes of learning more about the origins of our own planet Earth. Huygens will provide the first direct sampling of Titan's atmospheric chemistry and the first detailed photographs of its surface. The Cassini mission is an international effort involving NASA, the European Space Agency (ESA) and the Italian Space Agency, Agenzia Spaziale Italiana (ASI). The Jet Propulsion Laboratory manages the U.S. contribution to the mission for NASA's Office of Space Science. The major U.S. contractor is Lockheed Martin, which provided the launch vehicle and upper stage, spacecraft propulsion module and radioisotope thermoelectric generators that will provide power for the spacecraft. The Titan IV/Centaur is a U.S. Air Force launch vehicle, and launch operations were managed by the 45th Space Wing

KSC-97PC1545

NASA Image and Video Library

1997-10-15

A seven-year journey to the ringed planet Saturn begins with the liftoff of a Titan IVB/Centaur carrying the Cassini orbiter and its attached Huygens probe. Launch occurred at 4:43 a.m. EDT, Oct. 15, from Launch Complex 40 on Cape Canaveral Air Station. After a 2.2-billion mile journey that will include two swingbys of Venus and one of Earth to gain additional velocity, the two-story tall spacecraft will arrive at Saturn in July 2004. The orbiter will circle the planet for four years, its complement of 12 scientific instruments gathering data about Saturn's atmosphere, rings and magnetosphere and conducting closeup observations of the Saturnian moons. Huygens, with a separate suite of six science instruments, will separate from Cassini to fly on a ballistic trajectory toward Titan, the only celestial body besides Earth to have an atmosphere rich in nitrogen. Scientists are eager to study further this chemical similarity in hopes of learning more about the origins of our own planet Earth. Huygens will provide the first direct sampling of Titan's atmospheric chemistry and the first detailed photographs of its surface. The Cassini mission is an international effort involving NASA, the European Space Agency (ESA) and the Italian Space Agency, Agenzia Spaziale Italiana (ASI). The Jet Propulsion Laboratory manages the U.S. contribution to the mission for NASA's Office of Space Science. The major U.S. contractor is Lockheed Martin, which provided the launch vehicle and upper stage, spacecraft propulsion module and radioisotope thermoelectric generators that will provide power for the spacecraft. The Titan IV/Centaur is a U.S. Air Force launch vehicle, and launch operations were managed by the 45th Space Wing

Hourly mass and snow energy balance measurements from Mammoth Mountain, CA USA, 2011-2017

NASA Astrophysics Data System (ADS)

Bair, Edward H.; Davis, Robert E.; Dozier, Jeff

2018-03-01

The mass and energy balance of the snowpack govern its evolution. Direct measurement of these fluxes is essential for modeling the snowpack, yet there are few sites where all the relevant measurements are taken. Mammoth Mountain, CA USA, is home to the Cold Regions Research and Engineering Laboratory and University of California - Santa Barbara Energy Site (CUES), one of five energy balance monitoring sites in the western US. There is a ski patrol study site on Mammoth Mountain, called the Sesame Street Snow Study Plot, with automated snow and meteorological instruments where new snow is hand-weighed to measure its water content. There is also a site at Mammoth Pass with automated precipitation instruments. For this dataset, we present a clean and continuous hourly record of selected measurements from the three sites covering the 2011-2017 water years. Then, we model the snow mass balance at CUES and compare model runs to snow pillow measurements. The 2011-2017 period was marked by exceptional variability in precipitation, even for an area that has high year-to-year variability. The driest year on record, and one of the wettest years, occurred during this time period, making it ideal for studying climatic extremes. This dataset complements a previously published dataset from CUES containing a smaller subset of daily measurements. In addition to the hand-weighed SWE, novel measurements include hourly broadband snow albedo corrected for terrain and other measurement biases. This dataset is available with a digital object identifier: https://doi.org/10.21424/R4159Q.

Exploring Mercury's Surface in UltraViolet from Orbit

NASA Astrophysics Data System (ADS)

Izenberg, N.

2017-12-01

The MESSENGER Mission's Ultraviolet and Visible Spectrometer (UVVS) component of its Mercury Atmosphere and Surface Composition Spectrometer (MASCS) instrument obtained approximately 4600 point observations of Mercury's surface in middle ultraviolet (MUV; 210 nm - 300 nm) and far ultraviolet (FUV; 119.1 - 122.5 nm and 129.2 - 131.5 nm) wavelengths over the course of its orbital mission, mostly in Mercury's southern hemisphere. Given the very low (<1 to 2 wt %) average abundance of iron in the silicates of Mercury observed by multiple MESSENGER instruments, the near- to middle-ultraviolet wavelengths encompassing the oxygen metal charge transfer band (<400 nm), which is more sensitive to the presence of iron than the classic 1 micron absorption band, provides potentially useful additional compositional insight into the top layer of Mercury's regolith. The presence of nano- and microphase carbon also has potentially significant expression in the ultraviolet, and the interplay and variation between carbon and iron in mercury surface materials is an active area of investigation. Analysis of middle-UV surface reflectance and parameters appear to support the presence of varying amounts of carbon in different spectral or geologic units on Mercury. Far-UV reflectance data is currently under-utilized, but analysis of lunar surface by the Lunar Reconnaissance Orbiter (LRO) Lyman Alpha Mapping Project (LAMP) indicate that the data are sensitive to both composition and space weathering. The far-UV reflectance from MASCS may provide similar information for the Mercury surface, complementing results from longer wavelengths. MESSENGER data products for surface reflectance include middle-UV reflectance spectra, ultraviolet far-UV reflectance values, combined middle-UV through near-infrared spectra (210 nm - 1450 nm), a global `spectral cube' of near-UV to near-IR, and an upcoming UV spectral cube.

Seismic component of the STEEP project, Alaska: Results of the first field season

NASA Astrophysics Data System (ADS)

Hansen, R. A.; Estes, S.; Stachnik, J.; Lafevers, M.; Roush, J.; Sanches, R.; Fuerst, E.; Sandru, J.; Ruppert, N.; Pavlis, G.; Bauer, M.

2005-12-01

STEEP (SainT Elias Erosion/tectonics Project) is a five year, multi-disciplinary study that addresses evolution of the highest coastal mountain range on Earth - the St. Elias Mountains of southern Alaska and northwestern Canada. The overall goal of the project is to develop a comprehensive model for the St. Elias orogen that accounts for the interaction of regional plate tectonic processes, structural development, and rapid erosion. The seismic component of this project includes passive seismic experiment utilizing the IRIS PASSCAL Program instruments. The total project consists of 22 new, telemetered, digital broad band seismic stations, most accessible by helicopter only. There are 12 existing short period stations in the area. Eight new stations were installed in the coastal region in June 2005. Freewave IP radios provide the telemetry to the newly installed VSAT at the Bering Glacier camp site. The challenge was to find ice-free locations, on bedrock, large enough to install equipment and still have a helicopter landing zone nearby. The stations consist of Quanterra Q330 digitizers with baler, a STS-2 seismometer installed in a vault, a Freewave IP radio, a Scala 900 Mhz antenna, twenty 100 AH rechargeable batteries with a 2400AH backup Celair primary battery, and three solar panels mounted on hut. The acquired data is recorded in real time at the Alaska Earthquake Information Center located in Fairbanks and is incorporated into the standard data processing procedures. High quality data allows for more reliable automatic earthquake detections in the region with lower magnitude threshold. In addition to tectonic earthquakes, glacial events that occur within the vast ice fields of the region are also regularly detected. Broadband instruments complement regional broadband network for more reliable calculations of the regional moment tensors.