Sample records for interstate shellfish shippers

  1. 75 FR 28858 - Agency Information Collection (Purchase of Shellfish) Activities Under OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-24

    ... DEPARTMENT OF VETERANS AFFAIRS [OMB Control No. 2900-0589] Agency Information Collection (Purchase... 852.270-3, Purchase of Shellfish, requires that a firm furnishing shellfish to VA must ensure that the... of the approved producer or shipper. The information is used to ensure that shellfish purchased by VA...

  2. 78 FR 42820 - Agency Information Collection (Purchase of Shellfish) Activities Under OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-17

    ... DEPARTMENT OF VETERANS AFFAIRS [OMB Control No. 2900-0589] Agency Information Collection (Purchase...: Revision of a currently approved collection. Abstract: VAAR clause 852.270-3, Purchase of Shellfish... approved producer or shipper. The information is used to ensure that shellfish purchased by VA comes from a...

  3. 76 FR 64425 - Union Pacific Railroad Company-Abandonment Exemption-in Pottawattamie County, IA; Iowa Interstate...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-18

    ... trackage rights and UP's overhead trackage rights, UP will sell the UP lines to a shipper, Red Giant Oil Company (Red Giant), subject to a permanent access easement over the UP lines for another shipper, Midwest Walnut Company of Iowa (Midwest Walnut). Red Giant and Midwest Walnut are the only shippers on the UP...

  4. 26 CFR 1.281-3 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... Interstate Commerce Act. Thus, if any shareholder of the corporation, regardless of the class or percentage of stock owned, is not subject to the jurisdiction of the Interstate Commerce Commission under part I... of the Interstate Commerce Act and to the shippers and passengers of such railroad corporations one...

  5. 26 CFR 1.281-3 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... Interstate Commerce Act. Thus, if any shareholder of the corporation, regardless of the class or percentage of stock owned, is not subject to the jurisdiction of the Interstate Commerce Commission under part I... of the Interstate Commerce Act and to the shippers and passengers of such railroad corporations one...

  6. 26 CFR 1.281-3 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... Interstate Commerce Act. Thus, if any shareholder of the corporation, regardless of the class or percentage of stock owned, is not subject to the jurisdiction of the Interstate Commerce Commission under part I... of the Interstate Commerce Act and to the shippers and passengers of such railroad corporations one...

  7. 77 FR 25371 - Transportation of Household Goods in Interstate Commerce; Consumer Protection Regulations...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-30

    ... services when the shipper agrees to release the carrier from full liability for potential loss and damage... implemented a congressional directive to enhance consumer protection in cases of loss or damage that occur... the estimates they provide to prospective shippers: WARNING: If a moving company loses or damages your...

  8. 75 FR 18885 - Agency Information Collection Activities: Proposed Collection; Comments Requested

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-13

    ... of information collection under review: Interstate firearms shipment report of theft/loss. The...: Interstate Firearms Shipment Report of Theft/Loss. (3) Agency form number, if any, and the applicable... is part of a voluntary program in which the common carrier and/or shipper report losses or thefts of...

  9. 75 FR 6706 - Agency Information Collection Activities: Proposed Collection; Comments Requested

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-10

    ... of Information Collection Under Review: Interstate Firearms Shipment Report of Theft/Loss. The...: Interstate Firearms Shipment Report of Theft/Loss. (3) Agency form number, if any, and the applicable... a voluntary program in which the common carrier and/or shipper report losses or thefts of firearms...

  10. 75 FR 13211 - Proposed Information Collection (Purchase of Shellfish) Activity: Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-18

    ...: Submit written comments on the collection of information through Federal Docket Management System (FDMS...), Federal agencies must obtain approval from the Office of Management and Budget (OMB) for each collection... the State of origin, and the certificate number of the approved producer or shipper. The information...

  11. 21 CFR 1240.60 - Molluscan shellfish.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 8 2014-04-01 2014-04-01 false Molluscan shellfish. 1240.60 Section 1240.60 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED...) A person shall not offer for transportation, or transport, in interstate traffic any molluscan...

  12. 21 CFR 1240.60 - Molluscan shellfish.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 8 2013-04-01 2013-04-01 false Molluscan shellfish. 1240.60 Section 1240.60 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED...) A person shall not offer for transportation, or transport, in interstate traffic any molluscan...

  13. 21 CFR 1240.60 - Molluscan shellfish.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 8 2012-04-01 2012-04-01 false Molluscan shellfish. 1240.60 Section 1240.60 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED...) A person shall not offer for transportation, or transport, in interstate traffic any molluscan...

  14. 18 CFR 250.16 - Format of compliance plan for transportation services and affiliate transactions.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... distribution company, an interstate pipeline, an intrastate pipeline, an end-user, a producer, a marketer, or a... affiliate's role in the transportation transaction (i.e., shipper, marketer, supplier, seller); the duration...

  15. 78 FR 22366 - Proposed Information Collection (Purchase of Shellfish) Activity: Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-15

    ...: Submit written comments on the collection of information through Federal Docket Management System [[Page... agencies must obtain approval from the Office of Management and Budget (OMB) for each collection of... indicating the name and address of the approved producer or shipper, the name of the State of origin, and the...

  16. 49 CFR 375.513 - Must I give the individual shipper an opportunity to observe the weighing?

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... MOTOR CARRIER SAFETY REGULATIONS TRANSPORTATION OF HOUSEHOLD GOODS IN INTERSTATE COMMERCE; CONSUMER PROTECTION REGULATIONS Pick Up of Shipments of Household Goods Weighing the Shipment § 375.513 Must I give...

  17. 18 CFR 341.2 - Filing requirements.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ..., DEPARTMENT OF ENERGY REGULATIONS UNDER THE INTERSTATE COMMERCE ACT OIL PIPELINE TARIFFS: OIL PIPELINE... filings. (1) Carriers must serve tariff publications and justifications to each shipper and subscriber... for its inability to accept electronic service. (b) Notice period. All tariff publications (except for...

  18. 18 CFR 284.7 - Firm transportation service.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... include in its tariff any provision that inhibits the development of market centers. (c) Reasonable... as part of its transportation tariff. (d) Segmentation. An interstate pipeline that offers... purpose of releasing that capacity to replacement shippers to the extent such segmentation is...

  19. Recommended industry best management practices for the prevention of Phytophthora ramorum introduction in nursery operations

    Treesearch

    Karen Suslow

    2008-01-01

    The following industry recommended best management practices (BMPs), designed for growers and/or interstate shippers of host and associated host plants of Phytophthora ramorum, consists of biosecurity guidelines created by and for nursery growers in order to reduce the risks associated with P. ramorum. The control of P....

  20. 77 FR 32631 - Lion Oil Trading & Transportation, Inc., Magnolia Pipeline Company, and El Dorado Pipeline...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-01

    ..., requested that the Commission grant a temporary waiver of the Interstate Commerce Act (ICA) Section 6 and.... Aron is not a traditional transportation agreement with an unaffiliated shipper, and that even though J. Aron holds title to the throughput, the complex arrangement serves as an alternative to traditional...

  1. Deliverability on the interstate natural gas pipeline system

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    NONE

    1998-05-01

    Deliverability on the Interstate Natural Gas Pipeline System examines the capability of the national pipeline grid to transport natural gas to various US markets. The report quantifies the capacity levels and utilization rates of major interstate pipeline companies in 1996 and the changes since 1990, as well as changes in markets and end-use consumption patterns. It also discusses the effects of proposed capacity expansions on capacity levels. The report consists of five chapters, several appendices, and a glossary. Chapter 1 discusses some of the operational and regulatory features of the US interstate pipeline system and how they affect overall systemmore » design, system utilization, and capacity expansions. Chapter 2 looks at how the exploration, development, and production of natural gas within North America is linked to the national pipeline grid. Chapter 3 examines the capability of the interstate natural gas pipeline network to link production areas to market areas, on the basis of capacity and usage levels along 10 corridors. The chapter also examines capacity expansions that have occurred since 1990 along each corridor and the potential impact of proposed new capacity. Chapter 4 discusses the last step in the transportation chain, that is, deliverability to the ultimate end user. Flow patterns into and out of each market region are discussed, as well as the movement of natural gas between States in each region. Chapter 5 examines how shippers reserve interstate pipeline capacity in the current transportation marketplace and how pipeline companies are handling the secondary market for short-term unused capacity. Four appendices provide supporting data and additional detail on the methodology used to estimate capacity. 32 figs., 15 tabs.« less

  2. Detection of pathogenic Vibrio spp. in shellfish by using multiplex PCR and DNA microarrays.

    PubMed

    Panicker, Gitika; Call, Douglas R; Krug, Melissa J; Bej, Asim K

    2004-12-01

    This study describes the development of a gene-specific DNA microarray coupled with multiplex PCR for the comprehensive detection of pathogenic vibrios that are natural inhabitants of warm coastal waters and shellfish. Multiplex PCR with vvh and viuB for Vibrio vulnificus, with ompU, toxR, tcpI, and hlyA for V. cholerae, and with tlh, tdh, trh, and open reading frame 8 for V. parahaemolyticus helped to ensure that total and pathogenic strains, including subtypes of the three Vibrio spp., could be detected and discriminated. For DNA microarrays, oligonucleotide probes for these targeted genes were deposited onto epoxysilane-derivatized, 12-well, Teflon-masked slides by using a MicroGrid II arrayer. Amplified PCR products were hybridized to arrays at 50 degrees C and detected by using tyramide signal amplification with Alexa Fluor 546 fluorescent dye. Slides were imaged by using an arrayWoRx scanner. The detection sensitivity for pure cultures without enrichment was 10(2) to 10(3) CFU/ml, and the specificity was 100%. However, 5 h of sample enrichment followed by DNA extraction with Instagene matrix and multiplex PCR with microarray hybridization resulted in the detection of 1 CFU in 1 g of oyster tissue homogenate. Thus, enrichment of the bacterial pathogens permitted higher sensitivity in compliance with the Interstate Shellfish Sanitation Conference guideline. Application of the DNA microarray methodology to natural oysters revealed the presence of V. vulnificus (100%) and V. parahaemolyticus (83%). However, V. cholerae was not detected in natural oysters. An assay involving a combination of multiplex PCR and DNA microarray hybridization would help to ensure rapid and accurate detection of pathogenic vibrios in shellfish, thereby improving the microbiological safety of shellfish for consumers.

  3. Detection of Pathogenic Vibrio spp. in Shellfish by Using Multiplex PCR and DNA Microarrays

    PubMed Central

    Panicker, Gitika; Call, Douglas R.; Krug, Melissa J.; Bej, Asim K.

    2004-01-01

    This study describes the development of a gene-specific DNA microarray coupled with multiplex PCR for the comprehensive detection of pathogenic vibrios that are natural inhabitants of warm coastal waters and shellfish. Multiplex PCR with vvh and viuB for Vibrio vulnificus, with ompU, toxR, tcpI, and hlyA for V. cholerae, and with tlh, tdh, trh, and open reading frame 8 for V. parahaemolyticus helped to ensure that total and pathogenic strains, including subtypes of the three Vibrio spp., could be detected and discriminated. For DNA microarrays, oligonucleotide probes for these targeted genes were deposited onto epoxysilane-derivatized, 12-well, Teflon-masked slides by using a MicroGrid II arrayer. Amplified PCR products were hybridized to arrays at 50°C and detected by using tyramide signal amplification with Alexa Fluor 546 fluorescent dye. Slides were imaged by using an arrayWoRx scanner. The detection sensitivity for pure cultures without enrichment was 102 to 103 CFU/ml, and the specificity was 100%. However, 5 h of sample enrichment followed by DNA extraction with Instagene matrix and multiplex PCR with microarray hybridization resulted in the detection of 1 CFU in 1 g of oyster tissue homogenate. Thus, enrichment of the bacterial pathogens permitted higher sensitivity in compliance with the Interstate Shellfish Sanitation Conference guideline. Application of the DNA microarray methodology to natural oysters revealed the presence of V. vulnificus (100%) and V. parahaemolyticus (83%). However, V. cholerae was not detected in natural oysters. An assay involving a combination of multiplex PCR and DNA microarray hybridization would help to ensure rapid and accurate detection of pathogenic vibrios in shellfish, thereby improving the microbiological safety of shellfish for consumers. PMID:15574946

  4. Onboard screening dockside testing as a new means of managing paralytic shellfish poisoning risks in federally closed waters

    NASA Astrophysics Data System (ADS)

    DeGrasse, Stacey; Conrad, Stephen; DiStefano, Paul; Vanegas, Camilo; Wallace, David; Jensen, Pete; Hickey, J. Michael; Cenci, Florence; Pitt, Jaclyn; Deardorff, Dave; Rubio, Fernando; Easy, Dorothy; Donovan, Mary Anne; Laycock, Maurice; Rouse, Debbie; Mullen, John

    2014-05-01

    Paralytic shellfish poisoning (PSP) is the foodborne intoxication associated with the consumption of seafood contaminated with naturally occurring neurotoxins known as paralytic shellfish toxins. To protect public health from this potentially fatal syndrome, harvesting closures are implemented when toxins exceed the regulatory action level. Traditional monitoring programs established by state shellfish authorities allow for timely closures in state waters with minimal negative impacts on industry. However, such monitoring programs are not feasible in federal offshore waters given their distance from shore and the range of their spatial coverage. Thus innovative management strategies were investigated for these offshore resources. Georges Bank, an offshore resource with an estimated market value of more than 3 billion in Atlantic surfclams and ocean quahogs, has been closed to harvesting following a temporary ban in 1989 and a subsequent indefinite closure in 1990 due to the risk of PSP. As a means of managing this risk and allowing harvest of safe shellfish from this important resource, the Onboard Screening Dockside Testing Protocol (referred to as the Protocol) was developed by the US Food and Drug Administration (FDA), National Marine Fisheries Service (NMFS), state shellfish control authorities, and industry. The Protocol, which sets forth control measures to ensure product safety and public health protection, was endorsed by the Interstate Shellfish Sanitation Conference (ISSC) for pilot testing. Briefly, the pilot study Protocol required that (1) the fishing vessel receive a permit from NMFS to harvest in closed waters, (2) a miniμm of five shellfish samples per intended harvest lot be tested for PSP toxins onboard, and (3) harvesting only occur when the samples tested from the intended fishing area are negative using the Jellett Rapid Tests or Abraxis Shipboard ELISA kits. Finally, product landed under the Protocol was confirmed to be safe for consumption using the mouse bioassay (MBA) prior to its introduction into commerce. This paper presents data from the pilot study, with primary focus on the advantages and challenges of the field kits employed onboard compared to the dockside MBA, which has served as the longstanding regulatory method for PSP toxins. In 2010 alone, the successful pilot study resulted in the safe harvest of over 2.7 million worth of surfclams in an area that has otherwise been unavailable for decades. Due to the success of this pilot study, the Protocol was adopted into the National Shellfish Sanitation Program Model Ordinance as an approved marine biotoxin control strategy for use in federal waters at the 2011 ISSC Biennial Meeting. In January 2013 a portion of Georges Bank was reopened for the harvest of Atlantic surfclams and ocean quahogs to fishermen following the Protocol.

  5. 49 CFR 375.219 - May I extend credit to shippers?

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 5 2010-10-01 2010-10-01 false May I extend credit to shippers? 375.219 Section... Services to My Customers Collecting Transportation Charges § 375.219 May I extend credit to shippers? You may extend credit to shippers, but, if you do, it must be in accordance with § 375.807. ...

  6. 49 CFR 375.219 - May I extend credit to shippers?

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 49 Transportation 5 2014-10-01 2014-10-01 false May I extend credit to shippers? 375.219 Section... Services to My Customers Collecting Transportation Charges § 375.219 May I extend credit to shippers? You may extend credit to shippers, but, if you do, it must be in accordance with § 375.807. ...

  7. 49 CFR 375.219 - May I extend credit to shippers?

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 49 Transportation 5 2013-10-01 2013-10-01 false May I extend credit to shippers? 375.219 Section... Services to My Customers Collecting Transportation Charges § 375.219 May I extend credit to shippers? You may extend credit to shippers, but, if you do, it must be in accordance with § 375.807. ...

  8. 49 CFR 375.219 - May I extend credit to shippers?

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 49 Transportation 5 2012-10-01 2012-10-01 false May I extend credit to shippers? 375.219 Section... Services to My Customers Collecting Transportation Charges § 375.219 May I extend credit to shippers? You may extend credit to shippers, but, if you do, it must be in accordance with § 375.807. ...

  9. 49 CFR 375.219 - May I extend credit to shippers?

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 49 Transportation 5 2011-10-01 2011-10-01 false May I extend credit to shippers? 375.219 Section... Services to My Customers Collecting Transportation Charges § 375.219 May I extend credit to shippers? You may extend credit to shippers, but, if you do, it must be in accordance with § 375.807. ...

  10. 49 CFR 371.117 - Must I provide individual shippers with my policies concerning cancellation, deposits, and refunds?

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... event the shipper cancels an order for service before the date an authorized household goods motor carrier has been scheduled to pick up the shipper's property. (b) You must maintain records showing each... include: (1) Proof that the individual shipper cashed or deposited the check or money order, if the...

  11. 49 CFR 172.204 - Shipper's certification.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 2 2010-10-01 2010-10-01 false Shipper's certification. 172.204 Section 172.204 Transportation Other Regulations Relating to Transportation PIPELINE AND HAZARDOUS MATERIALS SAFETY... SECURITY PLANS Shipping Papers § 172.204 Shipper's certification. (a) General. Except as provided in...

  12. 49 CFR 173.22 - Shipper's responsibility.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 2 2010-10-01 2010-10-01 false Shipper's responsibility. 173.22 Section 173.22... SHIPMENTS AND PACKAGINGS Preparation of Hazardous Materials for Transportation § 173.22 Shipper's... converted, the manufacturer's certification, specification, approval, or exemption or special permit marking...

  13. 76 FR 16604 - Certain Preserved Mushrooms From the People's Republic of China: Final Results and Final...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-24

    ... New Shipper Reviews AGENCY: Import Administration, International Trade Administration, Department of... (the Department) published in the Federal Register the preliminary results of the new shipper reviews...: Preliminary Results of Antidumping Duty New Shipper Reviews, 75 FR 66729 (October 29, 2010) (Preliminary...

  14. 76 FR 74044 - Honey From Argentina: Final Results of Antidumping Duty New Shipper Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-30

    ... DEPARTMENT OF COMMERCE International Trade Administration [A-357-812] Honey From Argentina: Final...) published its preliminary results of the 2009-2010 new shipper review of the antidumping duty order on honey... Preliminary Results. \\1\\ See Honey From Argentina: Preliminary Results of Antidumping Duty New Shipper Review...

  15. 75 FR 61425 - Raw Flexible Magnets from the People's Republic of China: Rescission of New Shipper Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-05

    ... DEPARTMENT OF COMMERCE International Trade Administration [A-570-922] Raw Flexible Magnets from... the initiation of a new shipper review of the antidumping duty order on raw flexible magnets from the... forth in 19 CFR 351.214(b) and initiated an antidumping duty new shipper review. See Raw Flexible...

  16. 9 CFR 201.39 - Payment to be made to consignor or shipper by market agencies; exceptions.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... shipper by market agencies; exceptions. 201.39 Section 201.39 Animals and Animal Products GRAIN INSPECTION... shipper by market agencies; exceptions. (a) No market agency shall, except as provided in paragraph (b) of... from the Secretary or a court of competent jurisdiction, unless (1) such market agency has reason to...

  17. 77 FR 65669 - Glycine From the People's Republic of China: Notice of Initiation of Antidumping Duty New Shipper...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-30

    ... antidumping duty new shipper review of Hebei Donghua Jiheng Fine Chemical Co., Ltd. (Donghua Fine Chemical... timely filed request for a new shipper review from Donghua Fine Chemical and Hebei Donghua Jiheng Chemical Co., Ltd. (Donghua Chemical). In its request for a review, Donghua Fine Chemical identified itself...

  18. 75 FR 38080 - Certain Steel Nails From the People's Republic of China: Rescission of New Shipper Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-01

    ... People's Republic of China: Rescission of New Shipper Review AGENCY: Import Administration, International... initiation of a new shipper review of the antidumping duty order on certain steel nails from the People's Republic of China (``PRC'') covering the period of January 23, 2008, through July 31, 2009. On May 28, 2010...

  19. 75 FR 22742 - New Pneumatic Off-the-Road Tires From the People's Republic of China: Rescission of New Shipper...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-30

    ... DEPARTMENT OF COMMERCE International Trade Administration [A-570-912] New Pneumatic Off-the-Road... the initiation of a new shipper review of the antidumping duty order on new pneumatic off-the-road... Off-the-Road Tires from the People's Republic of China: Initiation of New Shipper Review, 74 FR 56575...

  20. 49 CFR 375.303 - If I sell liability insurance coverage, what must I do?

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ..., sell, or procure any type of insurance policy on behalf of the individual shipper covering loss or... insurance coverage for loss or damage to shipments: (1) You must issue to the individual shipper a policy or... a copy of the policy or other appropriate evidence to the individual shipper at the time you sell or...

  1. 75 FR 55307 - Honey From the People's Republic of China: Preliminary Intent To Rescind New Shipper Reviews

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-10

    ... Shipper Review, 75 FR 38980 (July 7, 2010). Expansion of the POR When the sale of the subject merchandise... review is based on a single sale, exclusion of that sale as non-bona fide necessarily must end the review... Shipper Review of Honey from the People's Republic of China: Bona Fide Analysis of the Sale Under Review...

  2. Integration of three echelon supply chain (supplier-manufacturer-distributor-drop shipper) with permissible delay in payment and penalty contract

    NASA Astrophysics Data System (ADS)

    Ibrahim, M. F.; Suparno

    2018-04-01

    Supply Chain Management (SCM) has to be considered in the company in order to improve the sustainability and competitiveness. SCM executed to integrating any companies on the supply chain in a way of coordinating the flow of goods, information, and financial. Permissible delay in payment is one of the coordination ways with allowing the costumers delay the payments to a vendor in some certain periods without any interest charges. In the supply chain system, drop-shipping player already familiar in this era. In drop-shipping internet retailing, the supplier will hold supplies and also carry out physical distribution service on behalf of drop-shipper. Drop-shipper will just focus on selling, on the other hand, their supplier will be responsible for the physical process. Generally, drop-shipper have information of the customer demands better than the distributor. But, it is also unrare when the drop-shipper send the estimation of demands which bigger than their own estimation in order to maximize their own interest, so they hope supplies of the distributor will always enough to accommodate their demands. Contributions in this research will be focused on integration of three echelons supply chain, which are the supplier, manufacturer, distributor, and drop-shipper. With considering delay in payment on first and second echelons, and also the contract penalty on third echelon. The problem on this research will be modeled in some kind of cases which can represent the problem of real supply chain system. Sensitivity analysis will be done on certain significant variables toward the changes of total supply chain cost. Coordination with delay in payment success to integrate supply chain. Contract penalty plan success to maintain the profit of distributor and drop-shipper.

  3. 7 CFR 917.37 - Assessments.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... the functions of the commodity committee prior to commencement of shipments in any season, shippers may make advance payments of assessments, which advance payments shall be credited to such shippers...

  4. Use of advanced modeling techniques to optimize thermal packaging designs.

    PubMed

    Formato, Richard M; Potami, Raffaele; Ahmed, Iftekhar

    2010-01-01

    Through a detailed case study the authors demonstrate, for the first time, the capability of using advanced modeling techniques to correctly simulate the transient temperature response of a convective flow-based thermal shipper design. The objective of this case study was to demonstrate that simulation could be utilized to design a 2-inch-wall polyurethane (PUR) shipper to hold its product box temperature between 2 and 8 °C over the prescribed 96-h summer profile (product box is the portion of the shipper that is occupied by the payload). Results obtained from numerical simulation are in excellent agreement with empirical chamber data (within ±1 °C at all times), and geometrical locations of simulation maximum and minimum temperature match well with the corresponding chamber temperature measurements. Furthermore, a control simulation test case was run (results taken from identical product box locations) to compare the coupled conduction-convection model with a conduction-only model, which to date has been the state-of-the-art method. For the conduction-only simulation, all fluid elements were replaced with "solid" elements of identical size and assigned thermal properties of air. While results from the coupled thermal/fluid model closely correlated with the empirical data (±1 °C), the conduction-only model was unable to correctly capture the payload temperature trends, showing a sizeable error compared to empirical values (ΔT > 6 °C). A modeling technique capable of correctly capturing the thermal behavior of passively refrigerated shippers can be used to quickly evaluate and optimize new packaging designs. Such a capability provides a means to reduce the cost and required design time of shippers while simultaneously improving their performance. Another advantage comes from using thermal modeling (assuming a validated model is available) to predict the temperature distribution in a shipper that is exposed to ambient temperatures which were not bracketed during its validation. Thermal packaging is routinely used by the pharmaceutical industry to provide passive and active temperature control of their thermally sensitive products from manufacture through end use (termed the cold chain). In this study, the authors focus on passive temperature control (passive control does not require any external energy source and is entirely based on specific and/or latent heat of shipper components). As temperature-sensitive pharmaceuticals are being transported over longer distances, cold chain reliability is essential. To achieve reliability, a significant amount of time and resources must be invested in design, test, and production of optimized temperature-controlled packaging solutions. To shorten the cumbersome trial and error approach (design/test/design/test …), computer simulation (virtual prototyping and testing of thermal shippers) is a promising method. Although several companies have attempted to develop such a tool, there has been limited success to date. Through a detailed case study the authors demonstrate, for the first time, the capability of using advanced modeling techniques to correctly simulate the transient temperature response of a coupled conductive/convective-based thermal shipper. A modeling technique capable of correctly capturing shipper thermal behavior can be used to develop packaging designs more quickly, reducing up-front costs while also improving shipper performance.

  5. 49 CFR 1313.6 - Contract summary for agricultural commodities.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... corporate names of all carriers that are parties to the contract, and their addresses for service of... description in the contract. (3) Shipper identity. The specific identity of the shipper party to the contract...

  6. 49 CFR 1313.6 - Contract summary for agricultural commodities.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... corporate names of all carriers that are parties to the contract, and their addresses for service of... description in the contract. (3) Shipper identity. The specific identity of the shipper party to the contract...

  7. 49 CFR 1313.6 - Contract summary for agricultural commodities.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... corporate names of all carriers that are parties to the contract, and their addresses for service of... description in the contract. (3) Shipper identity. The specific identity of the shipper party to the contract...

  8. 49 CFR 1313.6 - Contract summary for agricultural commodities.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... corporate names of all carriers that are parties to the contract, and their addresses for service of... description in the contract. (3) Shipper identity. The specific identity of the shipper party to the contract...

  9. 49 CFR 1313.6 - Contract summary for agricultural commodities.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... corporate names of all carriers that are parties to the contract, and their addresses for service of... description in the contract. (3) Shipper identity. The specific identity of the shipper party to the contract...

  10. 78 FR 40100 - Certain Frozen Fish Fillets from the Socialist Republic of Vietnam: Rescission of Antidumping...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-03

    ...In response to a request from Ngoc Ha Co. Ltd. Food Processing and Trading (``Ngoc Ha''), the Department of Commerce (``the Department'') initiated a new shipper review of the antidumping duty order on certain frozen fish fillets from the Socialist Republic of Vietnam (``Vietnam'') covering the period August 1, 2012, through January 31, 2013.\\1\\ On May 23, 2013, Ngoc Ha withdrew its request for a new shipper review. Accordingly, the Department is rescinding the new shipper review with respect to Ngoc Ha. ---------------------------------------------------------------------------

  11. The Business Case for Becoming a SmartWay Shipper

    EPA Pesticide Factsheets

    This EPA presentation provides information on the SmartWay Partnership Program; what it is, how it works, benefits of becoming a SW registered shipper, why freight sustainability matters, and achieving sustainability goals throughout the supply chain.

  12. 7 CFR 60.133 - Wild fish and shellfish.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 3 2014-01-01 2014-01-01 false Wild fish and shellfish. 60.133 Section 60.133... FOR FISH AND SHELLFISH General Provisions Definitions § 60.133 Wild fish and shellfish. Wild fish and shellfish means naturally-born or hatchery-originated fish or shellfish released in the wild, and caught...

  13. 7 CFR 60.133 - Wild fish and shellfish.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 3 2012-01-01 2012-01-01 false Wild fish and shellfish. 60.133 Section 60.133... FOR FISH AND SHELLFISH General Provisions Definitions § 60.133 Wild fish and shellfish. Wild fish and shellfish means naturally-born or hatchery-originated fish or shellfish released in the wild, and caught...

  14. 7 CFR 60.133 - Wild fish and shellfish.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 3 2011-01-01 2011-01-01 false Wild fish and shellfish. 60.133 Section 60.133... FOR FISH AND SHELLFISH General Provisions Definitions § 60.133 Wild fish and shellfish. Wild fish and shellfish means naturally-born or hatchery-originated fish or shellfish released in the wild, and caught...

  15. 7 CFR 60.133 - Wild fish and shellfish.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 3 2013-01-01 2013-01-01 false Wild fish and shellfish. 60.133 Section 60.133... FOR FISH AND SHELLFISH General Provisions Definitions § 60.133 Wild fish and shellfish. Wild fish and shellfish means naturally-born or hatchery-originated fish or shellfish released in the wild, and caught...

  16. Impacts of CERCLA Release Notification Requirements on Transportation of Products Containing Hazardous Substances

    DOT National Transportation Integrated Search

    1986-08-01

    In order to determine the regulatory burden imposed by CERCLA release-notification requirements on shippers and carriers handling products containing hazardous substances, eight shippers and seven carriers were interviewed in depth during the summer ...

  17. 76 FR 70111 - Certain Frozen Fish Fillets from the Socialist Republic of Vietnam: Extension of Deadline for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-10

    ... completion of the preliminary results of a new shipper review to 300 days if it determines that the case is... shipper review involves extraordinarily complicated methodological issues. Interested parties have...

  18. 7 CFR 925.160 - Reports.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... and Orders; Fruits, Vegetables, Nuts), DEPARTMENT OF AGRICULTURE GRAPES GROWN IN A DESIGNATED AREA OF... Grape Administrative Committee, each shipper who ships grapes, shall furnish an end-of-season grape... requested by the California Desert Grape Administrative Committee (CDGAC), each shipper who ships grapes...

  19. 7 CFR 925.160 - Reports.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... and Orders; Fruits, Vegetables, Nuts), DEPARTMENT OF AGRICULTURE GRAPES GROWN IN A DESIGNATED AREA OF... Grape Administrative Committee, each shipper who ships grapes, shall furnish an end-of-season grape... requested by the California Desert Grape Administrative Committee (CDGAC), each shipper who ships grapes...

  20. 46 CFR 515.31 - General duties.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... principal or shipper without the express written authority of such principal or shipper. (i) Accounting to... MARITIME COMMISSION REGULATIONS AFFECTING OCEAN SHIPPING IN FOREIGN COMMERCE LICENSING, FINANCIAL...) have been reported to the Commission in writing; and (2) are covered by increased financial...

  1. [Epidemiology of toxic and infectious risk related to shellfish consumption].

    PubMed

    Desenclos, J C

    1996-10-01

    For feeding purposes shellfish filter large amounts of water but also concentrate infectious agents and toxins that are present in the marine environment either naturally or because of pollution. Thus, the consumption of raw or undercooked shellfish is a substantial source of foodborne poisoning, mostly epidemic and sometimes sporadic. Most of shellfish-borne infectious diseases are linked to fecal contamination of the marine environment; they include: thyphoid fever, salmonellosis, shigellosis, campylobacteriosis, cholera, Norwalk or Norwalk-like gastroenteritis and hepatitis A. In warm climates, shellfish contains naturally occurring halopilic Vibrios and may cause severe sporadic infections (septicemias) among very susceptible consumers (immunocompromised). Shellfish also causes outbreaks of paralytic shellfish poisoning (PSP) and diarrheic shellfish poisoning (DSP) when they are contaminated by toxins produced when Dinophisis, a marine plancton, proliferates. Chemical compounds (heavy metals and organic toxins) that are dumped in the environment (soil, air, and water) also reach shellfish harvesting waters where they are cocentrated. Although acute or chronic effects of the chemical contamination of shellfish have not been clearly documented, the cadmium pollution of some shellfish harvesting waters raises a serious problem. Since it is impossible to prevent completely the contamination of coastal waters by any of the agents cited above, the prevention of shellfish-borne diseases requires monitoring of the marine environment and shellfish flesh (coliform count, Dinophysis toxins, heavy metals...). This surveillance allows the classification of growing areas as suitable or not for harvesting and distribution of shellfish. However, this surveillance is not always sensitive enough. Indicators of fecal pollution are particularly not reliable for shellfish viral contamination. A better knowledge of marine biology, the limitation of coastal waters pollution, improved surveillance, the development of more sensitive indicators, the responsabilisation of the industry and the information of the public on the health hazards associated with shellfish consumption are the key issues for the improvement of shellfish-borne disease prevention.

  2. 76 FR 43262 - Tapered Roller Bearings and Parts Thereof, Finished and Unfinished, From the People's Republic of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-20

    ... preliminary intent to rescind the new shipper review (``NSR'') of Xiang Yang Automobile Bearing Co., Ltd... Rescind the New Shipper Review of Xiang Yang Automobile Bearing Co., Ltd. (``ZXY''), dated June 17, 2011...

  3. Shipper's guide for proper stowage of intermodal containers for ocean transport

    DOT National Transportation Integrated Search

    2000-01-01

    This short guide has been prepared in an effort to further the successful intermodal carriage of cargo in answer to the request of shippers. This booklet has been compiled as a generalized approach to intermodal transport and door-to-door shipments w...

  4. 77 FR 79 - Honey From the People's Republic of China: Preliminary Rescission of the Administrative Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-03

    ... of Antidumping Duty New Shipper Review of Hebei New Donghua Amino Acid Co., Ltd., 69 FR 47405, 47406... Shipper Review, 68 FR 1439, 1440 (January 10, 2003). \\14\\ See Hebei New Donghua Amino Acid Co., Ltd. v...

  5. 49 CFR 1580.101 - Rail security coordinator.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... the corporate level. (d) Each freight railroad carrier, rail hazardous materials shipper, and rail hazardous materials receiver required to have an RSC must provide to TSA the names, title, phone number(s... any of this information changes. (e) Each freight railroad carrier, rail hazardous materials shipper...

  6. 49 CFR 1580.101 - Rail security coordinator.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... the corporate level. (d) Each freight railroad carrier, rail hazardous materials shipper, and rail hazardous materials receiver required to have an RSC must provide to TSA the names, title, phone number(s... any of this information changes. (e) Each freight railroad carrier, rail hazardous materials shipper...

  7. 49 CFR 1580.101 - Rail security coordinator.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... the corporate level. (d) Each freight railroad carrier, rail hazardous materials shipper, and rail hazardous materials receiver required to have an RSC must provide to TSA the names, title, phone number(s... any of this information changes. (e) Each freight railroad carrier, rail hazardous materials shipper...

  8. 78 FR 6297 - Certain Frozen Fish Fillets From the Socialist Republic of Vietnam: Preliminary Results of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-30

    ...The Department of Commerce (``the Department'') is conducting four new shipper reviews (``NSRs'') of the antidumping duty order on certain frozen fish fillets from the Socialist Republic of Vietnam (``Vietnam''). The period of review (``POR'') is August 1, 2011, through January 31, 2012. The reviews cover four exporters of subject merchandise: Quang Minh Seafood Co., Ltd. (``Quang Minh''); Dai Thanh Seafoods Company Limited (``Dathaco''); Fatifish Company Limited (``Fatifish''); and Hoang Long Seafood Processing Co., Ltd. (``Hoang Long'') (collectively, the ``New Shipper Respondents''). The Department has preliminarily determined that the New Shipper Respondents did not sell subject merchandise at less than normal value (``NV'').

  9. 77 FR 36260 - Proposed Information Collection; Comment Request; Puget Sound Recreational Shellfish Harvesting...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-18

    ... characteristics of recreational shellfish harvesting participants; (2) The economic value of access to Puget Sound... reasons, and how these actions affect the value of shellfish harvesting. The Puget Sound Recreational Shellfish Harvesting Project (PSRSHP) will provide critical economic data related to recreational shellfish...

  10. 46 CFR 515.32 - Freight forwarder duties.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... FEDERAL MARITIME COMMISSION REGULATIONS AFFECTING OCEAN SHIPPING IN FOREIGN COMMERCE LICENSING, FINANCIAL... forwarder is a shipper or seller of goods in international commerce or affiliated with such an entity, the... notice on such items: This company is a shipper or seller of goods in international commerce or is...

  11. 78 FR 24723 - Freshwater Crawfish Tail Meat From the People's Republic of China: Initiation of Antidumping Duty...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-26

    ... Meat From the People's Republic of China: Initiation of Antidumping Duty New Shipper Review AGENCY... shipper review (NSR) of the antidumping duty order on freshwater crawfish tail meat from the People's... crawfish tail meat from the [[Page 24724

  12. 22 CFR 123.16 - Exemptions of general applicability.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... subchapter); MTCR articles; Significant Military Equipment (SME); and may not be used by persons who are..., including those to and from Canada, require a Shipper's Export Declaration (SED) or notification letter. If... § 123.22 for Shipper's Export Declaration and letter notification requirements. (b) The following...

  13. Fish and shellfish allergy.

    PubMed

    Wild, Laurianne G; Lehrer, Samuel B

    2005-01-01

    Fish and shellfish are important in the American diet and economy. Nearly $27 billion are spent each year in the United States on seafood products. Fish and shellfish are also important causes of food hypersensitivity. In fact, shellfish constitute the number one cause of food allergy in the American adult. During the past decade, much has been learned about allergens in fish and shellfish. The major allergens responsible for cross-reactivity among distinct species of fish and amphibians are parvalbumins. The major shellfish allergen has been identified as tropomyosin. Many new and important potential cross-reacting allergens have been identified within the fish family and between shellfish, arachnids, and insects. Extensive research is currently underway for the development of safer and more effective methods for the diagnosis and management of fish and shellfish hypersensitivity.

  14. Assessing public benefits and costs of freight transportation projects : measuring shippers' value of delay on the freight system.

    DOT National Transportation Integrated Search

    2012-07-01

    Freight delay is detrimental to the national economy. In an effort to gauge the economic impact of freight delay due : to highway congestion, this project focuses on estimating shippers value of delay (VOD). We have accomplished : this through thr...

  15. 76 FR 36909 - Commission Information Collection Activities (FERC-549B); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-23

    ... grid, the Commission revised its capacity release regulations regarding scheduling, segmentation and... market as well as to improve shipper's and the Commission's ability to monitor the market for potential... in a competitive market as well as improve shippers' and the Commission's ability to monitor...

  16. 7 CFR 201.23 - Name of shipper or consignee.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 3 2012-01-01 2012-01-01 false Name of shipper or consignee. 201.23 Section 201.23 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) FEDERAL SEED ACT FEDERAL SEED ACT REGULATIONS Labeling Agricultura...

  17. 7 CFR 201.27 - Name of shipper or consignee.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Name of shipper or consignee. 201.27 Section 201.27 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) FEDERAL SEED ACT FEDERAL SEED ACT REGULATIONS Labeling Vegetable...

  18. 7 CFR 201.27 - Name of shipper or consignee.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 3 2011-01-01 2011-01-01 false Name of shipper or consignee. 201.27 Section 201.27 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) FEDERAL SEED ACT FEDERAL SEED ACT REGULATIONS Labeling Vegetable...

  19. 7 CFR 201.23 - Name of shipper or consignee.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 3 2014-01-01 2014-01-01 false Name of shipper or consignee. 201.23 Section 201.23 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) FEDERAL SEED ACT FEDERAL SEED ACT REGULATIONS Labeling Agricultura...

  20. 7 CFR 201.23 - Name of shipper or consignee.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 3 2013-01-01 2013-01-01 false Name of shipper or consignee. 201.23 Section 201.23 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) FEDERAL SEED ACT FEDERAL SEED ACT REGULATIONS Labeling Agricultura...

  1. 7 CFR 201.27 - Name of shipper or consignee.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 3 2012-01-01 2012-01-01 false Name of shipper or consignee. 201.27 Section 201.27 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) FEDERAL SEED ACT FEDERAL SEED ACT REGULATIONS Labeling Vegetable...

  2. 7 CFR 201.27 - Name of shipper or consignee.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 3 2013-01-01 2013-01-01 false Name of shipper or consignee. 201.27 Section 201.27 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) FEDERAL SEED ACT FEDERAL SEED ACT REGULATIONS Labeling Vegetable...

  3. 7 CFR 201.27 - Name of shipper or consignee.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 3 2014-01-01 2014-01-01 false Name of shipper or consignee. 201.27 Section 201.27 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) FEDERAL SEED ACT FEDERAL SEED ACT REGULATIONS Labeling Vegetable...

  4. 7 CFR 201.23 - Name of shipper or consignee.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Name of shipper or consignee. 201.23 Section 201.23 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) FEDERAL SEED ACT FEDERAL SEED ACT REGULATIONS Labeling Agricultura...

  5. 7 CFR 201.23 - Name of shipper or consignee.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 3 2011-01-01 2011-01-01 false Name of shipper or consignee. 201.23 Section 201.23 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) FEDERAL SEED ACT FEDERAL SEED ACT REGULATIONS Labeling Agricultura...

  6. 76 FR 30910 - Polyethylene Terephthalate Film, Sheet, and Strip From India: Final Results of Countervailing...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-27

    ... Film, Sheet, and Strip From India: Final Results of Countervailing Duty New Shipper Review AGENCY... shipper review of polyethylene terephthalate film, sheet and strip (PET Film) from India for SRF Limited...-1398. SUPPLEMENTARY INFORMATION: Background Since the issuance of Polyethylene Terephthalate Film...

  7. 76 FR 50537 - Regulatory Guidance Concerning Household Goods Carriers Requiring Shippers To Sign Blank or...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-15

    ... Concerning Household Goods Carriers Requiring Shippers To Sign Blank or Incomplete Documents AGENCY: Federal.... Basis for This Notice The Agency has received numerous consumer complaints concerning household goods.... The FMCSA is issuing this regulatory guidance to eliminate any ambiguity or confusion concerning the...

  8. 76 FR 64426 - Notice of Railroad-Shipper Transportation Advisory Council Vacancy

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-18

    ... are voting members and are appointed from senior executive officers of organizations engaged in the... environment or the conservation of energy resources. Authority: 49 U.S.C. 726. Decided: October 12, 2011. By... (Board). ACTION: Notice of vacancies on the Railroad-Shipper Transportation Advisory Council (RSTAC) and...

  9. 78 FR 13396 - Notice of Railroad-Shipper Transportation Advisory Council Vacancy

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-27

    ...-No. 4). This action will not significantly affect either the quality of the human environment or the... (Board), Transportation. ACTION: Notice of vacancies on the Railroad-Shipper Transportation Advisory Council (RSTAC) and solicitation of nominations. SUMMARY: The Board hereby gives notice of one vacancy on...

  10. 75 FR 70080 - Notice of Railroad-Shipper Transportation Advisory Council Vacancies

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-16

    ... and are appointed from senior executive officers of organizations engaged in the railroad and rail... action will not significantly affect either the quality of the human environment or the conservation of... (Board), DOT. ACTION: Notice of vacancies on the Railroad-Shipper Transportation Advisory Council (RSTAC...

  11. 78 FR 40823 - Notice of Railroad-Shipper Transportation Advisory Council Vacancy

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-08

    ... are voting members and are appointed from senior executive officers of organizations engaged in the... the quality of the human environment or the conservation of energy resources. Authority: 49 U.S.C. 726.... ACTION: Notice of vacancies on the Railroad-Shipper Transportation Advisory Council (RSTAC) and...

  12. Changes in the Pipeline Transportation Market

    EIA Publications

    1999-01-01

    This analysis assesses the amount of capacity that may be turned back to pipeline companies, based on shippers' actions over the past several years and the profile of contracts in place as of July 1, 1998. It also examines changes in the characteristics of contracts between shippers and pipeline companies.

  13. 7 CFR 905.148 - Reports of special purpose shipments under certificates of privilege.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements and Orders; Fruits, Vegetables, Nuts... Regulations Non-Regulated Fruit § 905.148 Reports of special purpose shipments under certificates of privilege... name and address of the shipper or shippers; name and address of the certified organic Florida citrus...

  14. Contribution of Shellfish Consumption to Lower Mercury Health Risk for Residents in Northern Jiaozhou Bay, China.

    PubMed

    Zhang, Lei; Zhang, Lei

    2015-01-01

    Fish and marine mammal consumption are an important pathway for human exposure to mercury. The low mercury content in shellfish poses a low mercury health risk to people who consume shellfish. The objectives of this study are to detect mercury concentrations in different species of shellfish and to calculate the mercury health risk from shellfish consumption among traditional residents near northern Jiaozhou Bay. A total of 356 shellfish samples, which comprised 7 species from 5 different places in northern Jiaozhou Bay, were collected from April to June in 2012. The average mercury content in the collected shellfish ranged from 0.024 mg·kg(-1) to 0.452 mg·kg(-1). A total of 44 shellfish samples (12.36%) had mercury levels exceeding the national pollution-free aquatic products limit (0.3 mg·kg(-1)). Generally, the viscus had the highest mercury content among all parts of the shellfish. A positive correlation between mercury content and total weight/edible part weight was found in most species of the collected shellfish. The results showed that shellfish consumption resulted in the lower risk of mercury exposure to residents based on the calculation of daily intake (DI) and target hazard quotient (THQ).

  15. National collaborative shellfish pollution-indicator study: Site selection. Phase 2. Rept. for 1988-89

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Leonard, D.L.; Slaughter, E.A.; Corning, B.C.

    1990-07-01

    Each year, about 16 million areas of estuarine waters are classified for the harvest of molluscan shellfish as open or limited to harvest according to microbiological 'indicator' standards and pollution survey guidelines established by the National Shellfish Sanitation Program. The program was developed in the 1920s in response to typhoid fever outbreaks associated with shellfish consumption. Current microbiological indicator standards in shellfish and shellfish-growing waters are extrpolated from standards set in the 1920s. Results from studies in the last decade have indicated that these microbiological indicator standards and thus classification of shellfish-growing waters may no longer be valid. The Nationalmore » Collaborative Shellfish Pollution Indicator Study is proposed as a four-year study to evaluate the current relationships between indicators of human enteric pathogens and the incidence of shellfish-borne diseases. Tasks forces were established to address specific issues, including site selection, shoreline surveys, and laboratory methodologies.« less

  16. 77 FR 266 - Fresh Garlic From the People's Republic of China: Initiation of New Shipper Reviews

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-04

    ... DEPARTMENT OF COMMERCE International Trade Administration [A-570-831] Fresh Garlic From the People... determined that two requests for a new shipper review (NSR) under the antidumping duty order on fresh garlic.... SUPPLEMENTARY INFORMATION: Background The Department published the antidumping duty order on fresh garlic from...

  17. 76 FR 20317 - Diamond Sawblades and Parts Thereof From the People's Republic of China: Final Rescission of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-12

    ... new shipper review (``NSR'') of Pujiang Talent Diamond Tools Co., Ltd. (``PTDT'').\\1\\ We gave... Countervailing Duty Operations, Preliminary Intent to Rescind the New Shipper Review of Pujiang Talent Diamond.... [FR Doc. 2011-8742 Filed 4-11-11; 8:45 am] BILLING CODE 3510-DS-P ...

  18. 75 FR 38986 - Fresh Garlic From the People's Republic of China: Initiation of New Shipper Reviews

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-07

    ...'s Republic of China: Initiation of New Shipper Reviews AGENCY: Import Administration, International... on fresh garlic from the People's Republic of China (PRC), meet the statutory and regulatory... People's Republic of China, 59 FR 59209 (November 16, 1994) (Order). On May 28, 2010, we received timely...

  19. 77 FR 5773 - Chlorinated Isocyanurates From the People's Republic of China: Initiation of New Shipper Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-06

    ... From the People's Republic of China: Initiation of New Shipper Review AGENCY: Import Administration... isocyanurates (chlorinated isos) from the People's Republic of China (PRC). The Department determines that the... Antidumping Duty Order: Chlorinated Isocyanurates from the People's Republic of China, 70 FR 36561 (June 24...

  20. 75 FR 343 - Fresh Garlic From the People's Republic of China: Initiation of New Shipper Reviews

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-05

    ...'s Republic of China: Initiation of New Shipper Reviews AGENCY: Import Administration, International... from the People's Republic of China (PRC), meet the statutory and regulatory requirements for... Republic of China, 59 FR 59209 (November 16, 1994) (Order). On November 27, 2009, the Department received...

  1. 75 FR 60730 - Certain Frozen Warmwater Shrimp From the Socialist Republic of Vietnam: Initiation of Antidumping...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-01

    ... Shrimp From the Socialist Republic of Vietnam: Initiation of Antidumping Duty New Shipper Review AGENCY... new shipper review (``NSR'') of the antidumping duty order on certain frozen warmwater shrimp (``shrimp'') from the Socialist Republic of Vietnam (``Vietnam''), received on August 26, 2010, meets the...

  2. 76 FR 41760 - Certain Frozen Warmwater Shrimp From the Socialist Republic of Vietnam: Extension of Final...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-15

    ... Shrimp From the Socialist Republic of Vietnam: Extension of Final Results of Antidumping Duty New Shipper... new shipper review of certain frozen warmwater shrimp from the Socialist Republic of Vietnam (``Vietnam''). The review covers the period February 1, 2010, through July 31, 2010. DATES: Effective Date...

  3. 76 FR 61088 - Certain Frozen Fish Fillets From the Socialist Republic of Vietnam: Initiation of New Shipper...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-03

    ... From the Socialist Republic of Vietnam: Initiation of New Shipper Reviews AGENCY: Import Administration... antidumping duty order on certain frozen fish fillets from the Socialist Republic of Vietnam, received between... on certain frozen fish fillets from the Socialist Republic of Vietnam was published in the Federal...

  4. 78 FR 15683 - Certain Preserved Mushrooms From the People's Republic of China: Preliminary Results of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-12

    ... covered under this order are the species Agaricus bisporus and Agaricus bitorquis. ``Preserved Mushrooms... New Shipper Reviews 74 FR 14772 (April 1, 2009) unchanged at Certain Preserved Mushrooms from the People's Republic of China: Final Results of Antidumping Duty New Shipper Reviews 74 FR 28882 (June 18...

  5. 78 FR 18315 - Certain Preserved Mushrooms From the People's Republic of China: Final Rescission of Antidumping...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-26

    ... From the People's Republic of China: Final Rescission of Antidumping Duty New Shipper Review; 2011-2012... preliminary rescission of this new shipper review (NSR) of Shandong Yinfeng Rare Fungus Corporation Ltd... Preserved Mushrooms from the People's Republic of China: Preliminary Rescission of Antidumping Duty New...

  6. 78 FR 4126 - Certain Preserved Mushrooms From the People's Republic of China: Preliminary Rescission of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-18

    ... From the People's Republic of China: Preliminary Rescission of Antidumping Duty New Shipper Review... conducting a new shipper review (NSR) of the antidumping duty order on certain preserved mushrooms from the... order are the species Agaricus bisporus and Agaricus bitorquis. The merchandise subject to this order is...

  7. 76 FR 46270 - Certain Preserved Mushrooms From the People's Republic of China: Preliminary Results of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-02

    ... From the People's Republic of China: Preliminary Results of Antidumping Duty New Shipper Reviews AGENCY...: August 2, 2011. SUMMARY: The Department of Commerce (the Department) is currently conducting two new... Mushrooms From the People's Republic of China: Notice of Initiation of Antidumping Duty New Shipper Reviews...

  8. 75 FR 36632 - Diamond Sawblades and Parts Thereof from the People's Republic of China: Initiation of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-28

    ... Thereof from the People's Republic of China: Initiation of Antidumping Duty New Shipper Review AGENCY... new shipper review (``NSR'') of the antidumping duty order on diamond sawblades and parts thereof... statutory and regulatory requirements for initiation. The period of review (``POR'') for the NSR is January...

  9. 76 FR 775 - Diamond Sawblades and Parts Thereof From the People's Republic of China: Initiation of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-06

    ... Thereof From the People's Republic of China: Initiation of Antidumping Duty New Shipper Review AGENCY... new shipper review (``NSR'') of the antidumping duty order on diamond sawblades and parts thereof... the statutory and regulatory requirements for initiation. The period of review (``POR'') for this NSR...

  10. 15 CFR 732.5 - Steps regarding Shipper's Export Declaration or Automated Export System record, Destination...

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 2 2010-01-01 2010-01-01 false Steps regarding Shipper's Export... Section 732.5 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) BUREAU OF INDUSTRY AND SECURITY, DEPARTMENT OF COMMERCE EXPORT ADMINISTRATION REGULATIONS STEPS FOR USING...

  11. 19 CFR 122.76 - Shipper's Export Declarations and inspection certificates.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 19 Customs Duties 1 2012-04-01 2012-04-01 false Shipper's Export Declarations and inspection certificates. 122.76 Section 122.76 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY AIR COMMERCE REGULATIONS Documents Required for Clearance and Permission To Depart; Electronic Manifest...

  12. 19 CFR 122.76 - Shipper's Export Declarations and inspection certificates.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 19 Customs Duties 1 2011-04-01 2011-04-01 false Shipper's Export Declarations and inspection certificates. 122.76 Section 122.76 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY AIR COMMERCE REGULATIONS Documents Required for Clearance and Permission To Depart; Electronic Manifest...

  13. 19 CFR 122.76 - Shipper's Export Declarations and inspection certificates.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 19 Customs Duties 1 2013-04-01 2013-04-01 false Shipper's Export Declarations and inspection certificates. 122.76 Section 122.76 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY AIR COMMERCE REGULATIONS Documents Required for Clearance and Permission To Depart; Electronic Manifest...

  14. 19 CFR 122.76 - Shipper's Export Declarations and inspection certificates.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 19 Customs Duties 1 2014-04-01 2014-04-01 false Shipper's Export Declarations and inspection certificates. 122.76 Section 122.76 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY AIR COMMERCE REGULATIONS Documents Required for Clearance and Permission To Depart; Electronic Manifest...

  15. 49 CFR 1180.1 - General policy statement for merger or control of at least two Class I railroads.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... shippers, environmental safeguards, and fair working conditions for employees. The Board must ensure that... continues to operate in a competitive environment, its new efficiencies would be shared with shippers and...) Reduction of competition. Although in specific markets railroads operate in a highly competitive environment...

  16. 77 FR 74269 - Agency Information Collection Activities; Revision of a Currently-Approved Information Collection...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-13

    ... occasion. Estimated Total Annual Burden: 5,524,500 hours [Informational documents provided to prospective shippers at 43,500 hours + Written Cost estimates for prospective shippers at 4,620,000 hours + Service orders, bills of lading at 805,300 hours + In-transit service notifications at 22,600 hours + Complaint...

  17. 78 FR 52501 - Glycine From the People's Republic of China: Preliminary Rescission of Antidumping Duty New...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-23

    ... (PRC). The new shipper review covers Hebei Donghua Jiheng Fine Chemical Company, Ltd. (Donghua Fine Chemical) for the period of review March 1, 2012, through August 31, 2012. We have preliminarily determined... Chemical) (collectively, the Hebei Companies) submitted in the context of this new shipper review in...

  18. 77 FR 65171 - Fresh Garlic From the People's Republic of China: Preliminary Rescission of Antidumping Duty New...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-25

    ... detailed in the Preliminary Decision Memorandum, the Department finds that Fuyi's sales under review are...'s Republic of China: Preliminary Rescission of Antidumping Duty New Shipper Reviews; 2010-2011... Department of Commerce (Department) is conducting new shipper reviews (NSR) of the antidumping duty order on...

  19. 77 FR 65670 - Honey From Argentina: Final Rescission of Antidumping Duty New Shipper Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-30

    ... DEPARTMENT OF COMMERCE International Trade Administration [A-357-812] Honey From Argentina: Final... order on honey from Argentina for the period of December 1, 2010, through November 30, 2011.\\1\\ We...\\ See Honey from Argentina: Preliminary Rescission of Antidumping Duty New Shipper Review, 77 FR 45334...

  20. 76 FR 5332 - Honey From Argentina: Notice of Initiation of Antidumping Duty New Shipper Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-31

    ... DEPARTMENT OF COMMERCE International Trade Administration [A-357-812] Honey From Argentina: Notice... received a request for a new shipper review of the antidumping duty order on honey from Argentina. See Notice of Antidumping Duty Order: Honey From Argentina, 66 FR 63672 (December 10, 2001) (Order). In...

  1. Enteric virus and vibrio contamination of shellfish: intervention strategies

    USDA-ARS?s Scientific Manuscript database

    INTRODUCTION. Molluscan shellfish include oysters, clams, mussels, and cockles, which can cause illnesses from a variety of human pathogens. Enteric viruses, like norovirus and hepatitis A virus, are generally transmitted to shellfish through fecal contamination of shellfish harvesting areas, alth...

  2. 77 FR 61379 - Submission for OMB Review; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-09

    ... of recreational shellfish harvesting participants; (2) the economic value of access to Puget Sound..., and how these actions affect the value of shellfish harvesting. The Puget Sound Recreational Shellfish Harvesting Project (PSRSHP) will provide critical economic data related to recreational shellfish harvesting...

  3. 76 FR 775 - Fresh Garlic From the People's Republic of China: Extension of Time Limit for the Final Results...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-06

    ... DEPARTMENT OF COMMERCE International Trade Administration [A-570-831] Fresh Garlic From the People... Department) issued the preliminary results of the new shipper review of fresh garlic from the People's... Fresh Garlic From the People's Republic of China: Preliminary Results of New Shipper Reviews and...

  4. Logistics, Multimodal, and Shipper Partner 2.0.15 Tools: Guide to Importing Carrier Data Using the Outside Data Import Function 2015 Data Year - United States Version

    EPA Pesticide Factsheets

    This document provides guidance for Logistics, Multi-modal, and Shippers on how to use outside data collection systems to populate the SmartWay tools carrier data and activity sections using an automated method. (EPA publication # EPA-420-B-16-057a)

  5. 49 CFR 375.213 - What information must I provide to a prospective individual shipper?

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... hyperlink on your Internet Web site to the FMCSA Web site containing the information in FMCSA's publication... Internet Web site to the FMCSA Web site containing the information in FMCSA's publication “Your Rights and... explanation that individual shippers may examine these tariff sections or have copies sent to them upon...

  6. 49 CFR 375.217 - How must I collect charges upon delivery?

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... service and the bill of lading. (c) Charge or credit card payments: (1) If you agree to accept payment by charge or credit card, you must arrange with the individual shipper for the delivery only at a time when you can obtain authorization for the shipper's credit card transaction. (2) Paragraph (c)(1) of this...

  7. 78 FR 40428 - Fresh Garlic from the People's Republic of China: Initiation of Antidumping Duty New Shipper...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-05

    ...'s Republic of China: Initiation of Antidumping Duty New Shipper Review; 2011-2012 AGENCY: Import... fresh garlic from the People's Republic of China (PRC) meet the statutory and regulatory requirements... Republic of China, 59 FR 59209 (November 16, 1994). Qingshui and Merry each certified that each is both the...

  8. 78 FR 88 - Fresh Garlic From the People's Republic of China: Initiation of Antidumping Duty New Shipper...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-02

    ...'s Republic of China: Initiation of Antidumping Duty New Shipper Review; 2011-2012 AGENCY: Import... fresh garlic from the People's Republic of China (PRC) meets the statutory and regulatory requirements... Order: Fresh Garlic From the People's Republic of China, 59 FR 59209 (November 16, 1994). Pursuant to...

  9. 77 FR 26508 - Certain New Pneumatic Off-the-Road Tires From the People's Republic of China: Initiation of New...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-04

    ...-the-Road Tires From the People's Republic of China: Initiation of New Shipper Review AGENCY: Import... China (``PRC''), received on March 30, 2012, meets the statutory and regulatory requirements for... for a new shipper review from Trelleborg Wheel Systems (Xingtai) China Co. Ltd. (``TWS China'').\\2\\ On...

  10. 49 CFR 375.217 - How must I collect charges upon delivery?

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... service and the bill of lading. (c) Charge or credit card payments: (1) If you agree to accept payment by charge or credit card, you must arrange with the individual shipper for the delivery only at a time when you can obtain authorization for the shipper's credit card transaction. (2) Paragraph (c)(1) of this...

  11. 49 CFR 375.217 - How must I collect charges upon delivery?

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... service and the bill of lading. (c) Charge or credit card payments: (1) If you agree to accept payment by charge or credit card, you must arrange with the individual shipper for the delivery only at a time when you can obtain authorization for the shipper's credit card transaction. (2) Paragraph (c)(1) of this...

  12. 49 CFR 375.217 - How must I collect charges upon delivery?

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... service and the bill of lading. (c) Charge or credit card payments: (1) If you agree to accept payment by charge or credit card, you must arrange with the individual shipper for the delivery only at a time when you can obtain authorization for the shipper's credit card transaction. (2) Paragraph (c)(1) of this...

  13. 49 CFR 375.217 - How must I collect charges upon delivery?

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... service and the bill of lading. (c) Charge or credit card payments: (1) If you agree to accept payment by charge or credit card, you must arrange with the individual shipper for the delivery only at a time when you can obtain authorization for the shipper's credit card transaction. (2) Paragraph (c)(1) of this...

  14. 75 FR 20563 - Certain Frozen Warmwater Shrimp from the Socialist Republic of Vietnam: Extension of Final...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-20

    ... Shrimp from the Socialist Republic of Vietnam: Extension of Final Results of Antidumping Duty New Shipper... Vietnam (``Vietnam''). This review covers the period February 1, 2008 through January 31, 2009. EFFECTIVE... preliminary intent to rescind the new shipper review in the antidumping duty order on shrimp from Vietnam for...

  15. 75 FR 27705 - Certain Frozen Warmwater Shrimp from the Socialist Republic of Vietnam: Extension of Final...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-18

    ... Shrimp from the Socialist Republic of Vietnam: Extension of Final Results of Antidumping Duty New Shipper... Vietnam (``Vietnam''). This review covers the period February 1, 2008 through January 31, 2009. EFFECTIVE... preliminary intent to rescind the new shipper review in the antidumping duty order on shrimp from Vietnam for...

  16. 75 FR 339 - Wooden Bedroom Furniture From the People's Republic of China: Rescission of Antidumping Duty New...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-05

    ..., 2010. SUMMARY: In response to a request from Rise Furniture Co., Ltd. (``Rise''), the Department of..., 2009, through July 30, 2009.\\1\\ On November 10, 2009, Rise withdrew its request for a new shipper review. Accordingly, the Department is rescinding the new shipper review with respect to Rise. \\1\\ See...

  17. 76 FR 4634 - Diamond Sawblades and Parts Thereof From the People's Republic of China (PRC): Rescission of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-26

    ... Thereof From the People's Republic of China (PRC): Rescission of Antidumping Duty New-Shipper Review... (the Department) initiated a new-shipper review of the antidumping duty order on diamond sawblades and parts thereof from the People's Republic of China (PRC) covering the period January 23, 2009, through...

  18. 78 FR 37205 - Freshwater Crawfish Tail Meat From the People's Republic of China: Rescission of Antidumping Duty...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-20

    ... Meat From the People's Republic of China: Rescission of Antidumping Duty New Shipper Review AGENCY... freshwater crawfish tail meat from the People's Republic of China (PRC) with respect to Hubei Nature... Department initiated an antidumping duty new shipper review of freshwater crawfish tail meat from the PRC...

  19. 77 FR 65667 - Freshwater Crawfish Tail Meat From the People's Republic of China: Initiation of Antidumping Duty...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-30

    ... Meat From the People's Republic of China: Initiation of Antidumping Duty New Shipper Review AGENCY... new shipper review (``NSR'') of the antidumping duty order on freshwater crawfish tail meat from the... crawfish tail meat from the PRC published in the Federal Register on September 15, 1997.\\1\\ On September 28...

  20. 22 CFR 123.9 - Country of ultimate destination and approval of reexports or retransfers.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... destination on an application for an export license, or on a Shipper's Export Declaration where an exemption... the export license, or on the Shipper's Export Declaration in cases where an exemption is claimed... the invoice whenever defense articles on the U.S. Munitions List are to be exported: These commodities...

  1. 75 FR 63192 - Intent To Request Renewal From OMB of One Current Public Collection of Information: Air Cargo...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-14

    ... programs, security threat assessments (STA), known shipper data via the Known Shipper Management System... baggage, and other articles, that will be carried aboard a passenger aircraft; and (2) to establish a system to screen, inspect, report, or otherwise ensure the security of all cargo that is to be...

  2. 15 CFR 732.5 - Steps regarding Shipper's Export Declaration or Automated Export System record, Destination...

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... than anti-terrorism (AT). The only exception to this requirement would be the return of unwanted... be entered on the invoice and on the bill of lading, air waybill, or other export control document... THE EAR § 732.5 Steps regarding Shipper's Export Declaration or Automated Export System record...

  3. 15 CFR 732.5 - Steps regarding Shipper's Export Declaration or Automated Export System record, Destination...

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... than anti-terrorism (AT). The only exception to this requirement would be the return of unwanted... be entered on the invoice and on the bill of lading, air waybill, or other export control document... THE EAR § 732.5 Steps regarding Shipper's Export Declaration or Automated Export System record...

  4. 15 CFR 732.5 - Steps regarding Shipper's Export Declaration or Automated Export System record, Destination...

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... than anti-terrorism (AT). The only exception to this requirement would be the return of unwanted... be entered on the invoice and on the bill of lading, air waybill, or other export control document... THE EAR § 732.5 Steps regarding Shipper's Export Declaration or Automated Export System record...

  5. 15 CFR 732.5 - Steps regarding Shipper's Export Declaration or Automated Export System record, Destination...

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... than anti-terrorism (AT). The only exception to this requirement would be the return of unwanted... be entered on the invoice and on the bill of lading, air waybill, or other export control document... THE EAR § 732.5 Steps regarding Shipper's Export Declaration or Automated Export System record...

  6. 75 FR 61697 - Honey From the People's Republic of China: Extension of Time Limit for the Final Results for New...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-06

    ... DEPARTMENT OF COMMERCE International Trade Administration [A-570-863] Honey From the People's... (``Department'') initiated this new shipper review (``NSR'') of the antidumping duty order on honey from the.... See Honey from the People's Republic of China: Initiation of New Shipper Antidumping Duty Reviews, 75...

  7. A competitive ELISA to detect brevetoxins from Karenia brevis (formerly Gymnodinium breve) in seawater, shellfish, and mammalian body fluid.

    PubMed Central

    Naar, Jerome; Bourdelais, Andrea; Tomas, Carmelo; Kubanek, Julia; Whitney, Philip L; Flewelling, Leanne; Steidinger, Karen; Lancaster, Johnny; Baden, Daniel G

    2002-01-01

    We developed a competitive enzyme-linked immunosorbent assay (ELISA) to analyze brevetoxins, using goat anti-brevetoxin antibodies obtained after immunization with keyhole limpet hemocyanin-brevetoxin conjugates, in combination with a three-step signal amplification process. The procedure, which used secondary biotinylated antibodies, streptavidine-horseradish peroxidase conjugate, and chromogenic enzyme substrate, was useful in reducing nonspecific background signals commonly observed with complex matrices. This competitive ELISA detected brevetoxins in seawater, shellfish extract and homogenate, and mammalian body fluid such as urine and serum without pretreatment, dilution, or purification. We investigated the application of this technique for shellfish monitoring by spiking shellfish meat with brevetoxins and by analyzing oysters from two commercial shellfish beds in Florida that were exposed to a bloom of Karenia brevis (formerly Gymnodinium breve). We performed brevetoxin analysis of shellfish extracts and homogenates by ELISA and compared it with the mouse bioassay and receptor binding assay. The detection limit for brevetoxins in spiked oysters was 2.5 microg/100 g shellfish meat. This assay appears to be a useful tool for neurotoxic shellfish poisoning monitoring in shellfish and seawater, and for mammalian exposure diagnostics, and significantly reduces the time required for analyses. PMID:11836147

  8. Formation of a Volunteer Harmful Algal Bloom Network in British Columbia, Canada, Following an Outbreak of Diarrhetic Shellfish Poisoning

    PubMed Central

    McIntyre, Lorraine; Cassis, David; Haigh, Nicola

    2013-01-01

    Evidence for shellfish toxin illness in British Columbia (BC) on the west coast of Canada can be traced back to 1793. For over two hundred years, domestically acquired bivalve shellfish toxin illnesses in BC were solely ascribed to paralytic shellfish poisonings caused by algal blooms of Alexandrium. This changed in 2011, when BC experienced its first outbreak of diarrhetic shellfish poisoning (DSP). As a result of this outbreak, Canada’s first DSP symposium was held in November, 2012, in North Vancouver, BC. Three of the objectives of the symposium were to provide a forum to educate key stakeholders on this emerging issue, to identify research and surveillance priorities and to create a DSP network. The purpose of this paper is to review what is known about shellfish poisoning in BC and to describe a novel volunteer network that arose following the symposium. The newly formed network was designed for industry shellfish growers to identify harmful algae bloom events, so that they may take actions to mitigate the effects of harmful blooms on shellfish morbidity. The network will also inform public health and regulatory stakeholders of potentially emerging issues in shellfish growing areas. PMID:24172211

  9. Seafood-Associated Shellfish Allergy: A Comprehensive Review.

    PubMed

    Khora, Samanta S

    2016-08-01

    Shellfish are diverse, serve as main constituents of seafood, and are extensively consumed globally because of their nutritional values. Consequently, increase in reports of IgE-mediated seafood allergy is particularly food associated to shellfish. Seafood-associated shellfish consists of crustaceans (decapods, stomatopods, barnacles, and euphausiids) and molluskans (gastropods, bivalves, and cephalopods) and its products can start from mild local symptoms and lead to severe systemic anaphylactic reactions through ingestion, inhalation, or contact like most other food allergens. Globally, the most commonly causative shellfish are shrimps, crabs, lobsters, clams, oysters, and mussels. The prevalence of shellfish allergy is estimated to be 0.5-2.5% of the general population but higher in coastal Asian countries where shellfish constitute a large proportion of the diet. Diversity in allergens such as tropomyosin, arginine kinase, myosin light chain, and sarcoplasmic binding protein are from crustaceans whereas tropomyosin, paramyosin, troponin, actine, amylase, and hemoyanin are reported from molluskans shellfish. Tropomyosin is the major allergen and is responsible for cross-reactivity between shellfish and other invertebrates, within crustaceans, within molluskans, between crustaceans vs. molluskans as well as between shellfish and fish. Allergenicity diagnosis requires clinical history, in vivo skin prick testing, in vitro quantification of IgE, immunoCAP, and confirmation by oral challenge testing unless the reactions borne by it are life-threatening. This comprehensive review provides the update and new findings in the area of shellfish allergy including demographic, diversity of allergens, allergenicity, their cross-reactivity, and innovative molecular genetics approaches in diagnosing and managing this life-threatening as well as life-long disease.

  10. 48 CFR 852.270-3 - Purchase of shellfish.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Purchase of shellfish. 852.270-3 Section 852.270-3 Federal Acquisition Regulations System DEPARTMENT OF VETERANS AFFAIRS CLAUSES... Purchase of shellfish. As prescribed in 870.111-3, insert the following clause: Purchase of Shellfish (APR...

  11. 75 FR 34425 - Certain Steel Nails from the People's Republic of China: Final Results of the First New Shipper...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-17

    ... COMMENT 3: CLASSIFICATION OF CERTAIN INPUTS COMMENT 4: SURROGATE FINANCIAL RATIOS COMMENT 5: ADJUSTMENT TO... People's Republic of China, 73 FR 44961 (August 1, 2008) (``Order''). Based upon our analysis of the..., Analysis of the Final Results of the First New Shipper Review of Certain Steel Nails from the People's...

  12. 49 CFR 375.609 - What must I do for shippers who store household goods in transit?

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... transit? (a) If you are holding goods for storage-in-transit (SIT) and the period of time is about to... following four items: (1) The date of conversion to permanent storage. (2) The existence of a nine-month period after the date of conversion to permanent storage when the individual shipper may file claims...

  13. 49 CFR 375.609 - What must I do for shippers who store household goods in transit?

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... transit? (a) If you are holding goods for storage-in-transit (SIT) and the period of time is about to... following four items: (1) The date of conversion to permanent storage. (2) The existence of a nine-month period after the date of conversion to permanent storage when the individual shipper may file claims...

  14. 49 CFR 375.609 - What must I do for shippers who store household goods in transit?

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... transit? (a) If you are holding goods for storage-in-transit (SIT) and the period of time is about to... following four items: (1) The date of conversion to permanent storage. (2) The existence of a nine-month period after the date of conversion to permanent storage when the individual shipper may file claims...

  15. 49 CFR 375.609 - What must I do for shippers who store household goods in transit?

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... transit? (a) If you are holding goods for storage-in-transit (SIT) and the period of time is about to... following four items: (1) The date of conversion to permanent storage. (2) The existence of a nine-month period after the date of conversion to permanent storage when the individual shipper may file claims...

  16. 49 CFR 375.609 - What must I do for shippers who store household goods in transit?

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... transit? (a) If you are holding goods for storage-in-transit (SIT) and the period of time is about to... following four items: (1) The date of conversion to permanent storage. (2) The existence of a nine-month period after the date of conversion to permanent storage when the individual shipper may file claims...

  17. 77 FR 32522 - Tapered Roller Bearings and Parts Thereof, Finished and Unfinished From the People's Republic of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-01

    ... is renumbered as 8708.99.8180; see Id. Bona Fides Sale Analysis For this review, consistent with the... POR. In evaluating whether or not a sale in a new shipper review is commercially reasonable, and... Shipper Review, 67 FR 11283 (March 13, 2002)). The Department preliminarily finds that the sales of...

  18. 19 CFR 351.214 - New shipper reviews under section 751(a)(2)(B) of the Act.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... procedures for conducting such reviews. (b) Request for new shipper review—(1) Requirement of sale or export... notice of initiation of the requested review. (2) Absence of entry and sale to an unaffiliated customer...; and (ii) An expansion of the normal period of review to include an entry and sale to an unaffiliated...

  19. 75 FR 15412 - Silicon Metal From the People's Republic of China: Notice of Amended Final Results of New Shipper...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-29

    ... reviews to apply the recalculated surrogate value for the by-product silica fume in the Department's... specifically to the by-product silica fume. See Remand Order at 14. \\1\\ Respondents referenced here are (1... Redetermination of the Silica Fume By-Product Valuation, Remand for Antidumping Duty New Shipper Review of Silicon...

  20. Environmental Transmission of Human Noroviruses in Shellfish Waters

    PubMed Central

    Lees, David N.

    2014-01-01

    Human noroviruses (NoV) are the most common cause of epidemic gastroenteritis following consumption of bivalve shellfish contaminated with fecal matter. NoV levels can be effectively reduced by some sewage treatment processes such as activated sludge and membrane bioreactors. However, tertiary sewage treatment and substantial sewage dilution are usually required to achieve low concentrations of virus in shellfish. Most outbreaks have been associated with shellfish harvested from waters affected by untreated sewage from, for example, storm overflows or overboard disposal of feces from boats. In coastal waters, NoV can remain in suspension or associate with organic and inorganic matter and be accumulated by shellfish. Shellfish take considerably longer to purge NoV than fecal indicator bacteria when transferred from sewage-polluted estuarine waters to uncontaminated waters. The abundance and distribution of NoV in shellfish waters are influenced by the levels of sewage treatment, proximity of shellfish beds to sewage sources, rainfall, river flows, salinity, and water temperature. Detailed site-specific information on these factors is required to design measures to control the viral risk. PMID:24705321

  1. Case Report: Paralytic Shellfish Poisoning in Sabah, Malaysia.

    PubMed

    Suleiman, Maria; Jelip, Jenarun; Rundi, Christina; Chua, Tock H

    2017-12-01

    During the months of January-February and May-June 2013 coinciding with the red tide occurrence in Kota Kinabalu, Sabah, Malaysia, six episodes involving 58 cases of paralytic shellfish poisoning (PSP) or saxitoxin (STX) poisoning and resulting in four deaths were reported. Many of them were intoxicated from consuming shellfish purchased from the markets, whereas others were intoxicated from eating shellfish collected from the beach. Levels of STX in shellfish collected from the affected areas were high (mean 2,920 ± 780 and 360 ± 140 µg STX equivalents/100 g shellfish meat respectively for the two periods). The count of toxic dinoflagellates ( Pyrodinium bahamense var compressum ) of the sea water sampled around the coast was also high (mean 34,200 ± 10,300 cells/L). Species of shellfish containing high levels of STX were Atrina fragilis , Perna viridis , and Crassostrea belcheri . The age of victims varied from 9 to 67 years. Symptoms presented were typical of PSP, such as dizziness, numbness, vomiting, and difficulty in breathing. Recommended steps to prevent or reduce PSP in future red tide season include better monitoring of red tide occurrence, regular sampling of shellfish for determination of STX level, wider dissemination of information on the danger of eating contaminated shellfish among the communities, fishermen, and fishmongers.

  2. Molluscan shellfish allergy.

    PubMed

    Taylor, Steve L

    2008-01-01

    Food allergies affect approximately 3.5-4.0% of the worldwide population. Immediate-type food allergies are mediated by the production of IgE antibodies to specific proteins that occur naturally in allergenic foods. Symptoms are individually variable ranging from mild rashes and hives to life-threatening anaphylactic shock. Seafood allergies are among the most common types of food allergies on a worldwide basis. Allergies to fish and crustacean shellfish are very common. Molluscan shellfish allergies are well known but do not appear to occur as frequently. Molluscan shellfish allergies have been documented to all classes of mollusks including gastropods (e.g., limpet, abalone), bivalves (e.g., clams, oysters, mussels), and cephalopods (e.g., squid, octopus). Tropomyosin, a major muscle protein, is the only well-recognized allergen in molluscan shellfish. The allergens in oyster (Cra g 1), abalone (Hal m 1), and squid (Tod p 1) have been identified as tropomyosin. Cross-reactivity to tropomyosin from other molluscan shellfish species has been observed with sera from patients allergic to oysters, suggesting that individuals with allergies to molluscan shellfish should avoid eating all species of molluscan shellfish. Cross-reactions with the related tropomyosin allergens in crustacean shellfish may also occur but this is less clearly defined. Occupational allergies have also been described in workers exposed to molluscan shellfish products by the respiratory and/or cutaneous routes. With food allergies, one man's food may truly be another man's poison. Individuals with food allergies react adversely to the ingestion of foods and food ingredients that most consumers can safely ingest (Taylor and Hefle, 2001). The allergens that provoke adverse reactions in susceptible individuals are naturally occurring proteins in the specific foods (Bush and Hefle, 1996). Molluscan shellfish, like virtually all foods that contain protein, can provoke allergic reactions in some individuals.

  3. Strategic Mobility 21: Smart and Secure E Corridor Stakeholder Evaluation - Savannah

    DTIC Science & Technology

    2009-12-07

    validated to meet Customs-Trade Partnership Against Terrorism ( CTPAT ) level 3 Customs standards and combined with multiple overlain foreign trade zones... CTPAT level 3 benefits of technology validated supply chains insulating shippers against random inspections as the core elements of smart secure...their carrier partners, Customs-Trade Partnership Against Terrorism ( CTPAT ) level 3 technologically verified supply chain security for all shippers

  4. An oyster-associated hepatitis A outbreak in France in 2007.

    PubMed

    Guillois-Bécel, Y; Couturier, E; Le Saux, J C; Roque-Afonso, A M; Le Guyader, F S; Le Goas, A; Pernès, J; Le Bechec, S; Briand, A; Robert, C; Dussaix, E; Pommepuy, M; Vaillant, V

    2009-03-12

    Following the notification of nine hepatitis A cases clustered in the Cotes d Armor district in northwestern France, epidemiological, environmental and microbiological investigations were set up in order to identify the source and vehicle of contamination and implement control measures. In total, 111 cases were identified in the outbreak, all of whom lived or had stayed as tourists in the Cotes d Armor district. Of the cases, 87% had eaten raw shellfish, and 81% specifically oysters. Traceback investigations carried out on raw shellfish consumed by the cases showed that the raw shellfish originated from a single shellfish farm. The shellfish were probably contaminated either in the submersible tanks or in a depuration land-based tank where they were stored. The source of contamination was not identified but shellfish could have been tainted by sewage overflows or by wastewater releases from a polluted storm sewer close to the shellfish farm or from on-site sanitation facilities. To prevent future hepatitis A outbreaks due to shellfish consumption from this area, hazards specific to each farm should be analysed. Timely information on sewage overflows should also be part of communities efforts regarding sewage collection and treatment.

  5. Detection of Infectious Cryptosporidium parvum Oocysts in Mussels (Mytilus galloprovincialis) and Cockles (Cerastoderma edule)

    PubMed Central

    Gomez-Bautista, M.; Ortega-Mora, L. M.; Tabares, E.; Lopez-Rodas, V.; Costas, E.

    2000-01-01

    Infective Cryptosporidium parvum oocysts were detected in mussels (Mytilus galloprovincialis) and cockles (Cerastoderma edule) from a shellfish-producing region (Gallaecia, northwest Spain, bounded by the Atlantic Ocean) that accounts for the majority of European shellfish production. Shellfish were collected from bay sites with different degrees of organic pollution. Shellfish harboring C. parvum oocysts were recovered only from areas located near the mouths of rivers with a high density of grazing ruminants on their banks. An approximation of the parasite load of shellfish collected in positive sites indicated that each shellfish transported more than 103 oocysts. Recovered oocysts were infectious for neonatal mice, and PCR-restriction fragment length polymorphism analysis demonstrated a profile similar to that described for genotype C or 2 of the parasite. These results demonstrate that mussels and cockles could act as a reservoir of C. parvum infection for humans. Moreover, estuarine shellfish could be used as an indicator of river water contamination. PMID:10788352

  6. Evaluation of effects of shellfish aquaculture and capture fishery on a semi-closed bay ecosystem

    NASA Astrophysics Data System (ADS)

    Han, Dongyan; Chen, Yong; Zhang, Chongliang; Ren, Yiping; Xu, Binduo; Xue, Ying

    2018-07-01

    Coastal waters in the world are suffering from multiple anthropogenic disturbances, including capture fisheries and aquaculture. Ignoring possible interactions between multiple disturbances may cause serious consequences in our understanding of ecosystem dynamics. This study simulates individual and combined effects of fishing activities and shellfish cultivation on a semi-closed bay ecosystem using Ecopath with Ecosim (EwE). The changes of biomass and ecosystem structure were examined for three scenarios of having fishing alone, shellfish cultivation alone and both fishing and aquaculture. We found considerable impacts of shellfish aquaculture but minor impacts from fishing, suggesting shellfish aquaculture had dominated impacts on the study ecosystem. In addition, the effects of fishing and shellfish aquaculture were not additive on species biomass, rather showing a combined effect with synergy. Given the same loss in fisheries yield, controlling fishing and shellfish aquaculture simultaneously was more efficient for increasing nekton biomass compared to the control of either activity. We highlight the necessity for integrating management of multiple human stressors for an ecosystem-based management.

  7. New molecular methods for the detection of hepatitis A and Norwalk viruses in shellfish.

    PubMed

    Romalde, J L

    1996-12-01

    Outbreaks of viral enteric diseases after consumption of shellfish are a major health risk. Methodological problems (such as toxicity for cell cultures and low viral concentrations) and the unculturability of some strains (i.e. hepatitis A virus, Norwalk virus) have made it difficult to study those viruses in the environmental samples. Currently, the analysis of the hygienic quality of marketable shellfish is determined by the use of fecal indicator bacteria, but their reliability in determining viral pollution of shellfish is very low. Recent biotechnology developments are providing available rapid, sensitive, and specific tools for detecting food-borne viruses in shellfish and in shellfish-growing waters. In this paper, a review of these new molecular methods is carried out, discussing their advantages and possible applications.

  8. Recent trends in paralytic shellfish toxins in Puget Sound, relationships to climate, and capacity for prediction of toxic events

    Treesearch

    Stephanie K. Moore; Nathan J. Mantua; Barbara M. Hickey; Vera L. Trainer

    2009-01-01

    Temporal and spatial trends in paralytic shellfish toxins (PSTs) in Puget Sound shellfish and their relationships with climate are investigated using long-term monitoring data since 1957. Data are selected for trend analyses based on the sensitivity of shellfish species to PSTs and their depuration rates, and the frequency of sample collection at individual sites....

  9. Parallel Analyses of Alexandrium catenella Cell Concentrations and Shellfish Toxicity in the Puget Sound▿

    PubMed Central

    Dyhrman, Sonya T.; Haley, Sheean T.; Borchert, Jerry A.; Lona, Bob; Kollars, Nicole; Erdner, Deana L.

    2010-01-01

    Alexandrium catenella is widespread in western North America and produces a suite of potent neurotoxins that cause paralytic shellfish poisoning (PSP) in humans and have deleterious impacts on public health and economic resources. There are seasonal PSP-related closures of recreational and commercial shellfisheries in the Puget Sound, but the factors that influence cell distribution, abundance, and relationship to paralytic shellfish toxins (PSTs) in this system are poorly described. Here, a quantitative PCR assay was used to detect A. catenella cells in parallel with state shellfish toxicity testing during the 2006 bloom season at 41 sites from April through October. Over 500,000 A. catenella cells liter−1 were detected at several stations, with two main pulses of cells driving cell distribution, one in June and the other in August. PSTs over the closure limit of 80 μg of PST 100 per g of shellfish tissue were detected at 26 of the 41 sites. Comparison of cell numbers and PST data shows that shellfish toxicity is preceded by an increase in A. catenella cells in 71% of cases. However, cells were also observed in the absence of PSTs in shellfish, highlighting the complex relationship between A. catenella and the resulting shellfish toxicity. These data provide important information on the dynamics of A. catenella cells in the Puget Sound and are a first step toward assessing the utility of plankton monitoring to augment shellfish toxicity testing in this system. PMID:20495054

  10. A generic approach for the development of short-term predictions of Escherichia coli and biotoxins in shellfish

    PubMed Central

    Schmidt, Wiebke; Evers-King, Hayley L.; Campos, Carlos J. A.; Jones, Darren B.; Miller, Peter I.; Davidson, Keith; Shutler, Jamie D.

    2018-01-01

    Microbiological contamination or elevated marine biotoxin concentrations within shellfish can result in temporary closure of shellfish aquaculture harvesting, leading to financial loss for the aquaculture business and a potential reduction in consumer confidence in shellfish products. We present a method for predicting short-term variations in shellfish concentrations of Escherichia coli and biotoxin (okadaic acid and its derivates dinophysistoxins and pectenotoxins). The approach was evaluated for 2 contrasting shellfish harvesting areas. Through a meta-data analysis and using environmental data (in situ, satellite observations and meteorological nowcasts and forecasts), key environmental drivers were identified and used to develop models to predict E. coli and biotoxin concentrations within shellfish. Models were trained and evaluated using independent datasets, and the best models were identified based on the model exhibiting the lowest root mean square error. The best biotoxin model was able to provide 1 wk forecasts with an accuracy of 86%, a 0% false positive rate and a 0% false discovery rate (n = 78 observations) when used to predict the closure of shellfish beds due to biotoxin. The best E. coli models were used to predict the European hygiene classification of the shellfish beds to an accuracy of 99% (n = 107 observations) and 98% (n = 63 observations) for a bay (St Austell Bay) and an estuary (Turnaware Bar), respectively. This generic approach enables high accuracy short-term farm-specific forecasts, based on readily accessible environmental data and observations. PMID:29805719

  11. Surveillance of hepatitis A and E viruses contamination in shellfish in Thailand.

    PubMed

    Namsai, A; Louisirirotchanakul, S; Wongchinda, N; Siripanyaphinyo, U; Virulhakul, P; Puthavathana, P; Myint, K S; Gannarong, M; Ittapong, R

    2011-12-01

    To survey for hepatitis A virus (HAV) and hepatitis E virus (HEV) contamination in edible bivalve shellfish. A total of 213 shellfish (52 oysters, 69 cockles and 92 mussels) collected from a culture farm and two retailed markets were investigated for HAV and HEV contamination by reverse transcription-polymerase chain reaction (RT-PCR) assay using HA2-HA1 (capsid region) and HE366-HE363 (ORF2/3 overlapping region) primers, respectively. It was found that 3.8% of the shellfish and 2.9 and 6.5% of the cockle and mussel, respectively, showed positive for HAV detection. Nucleotide sequencing of all the 8 HAV-positive shellfish revealed 97-100% similarity to HAV subgenotype IA. Interestingly, viruses were found more frequently in the gills than in digestive tissue (4.5%vs 0.5%, P = 0.045). All the shellfish were negative for HEV. Significant contamination of HAV in edible bivalve shellfish was observed. Beside digestive tissue, gills are one of the important samples for viral genome detection. HAV-contaminated shellfish can play a role as reservoirs and/or vehicles in faecal-oral transmission in Thailand, and further monitoring of such a contamination is required. © 2011 The Authors. Letters in Applied Microbiology © 2011 The Society for Applied Microbiology.

  12. Not all shellfish "allergy" is allergy!

    PubMed Central

    2011-01-01

    The popularity of shellfish has been increasing worldwide, with a consequent increase in adverse reactions that can be allergic or toxic. The approximate prevalence of shellfish allergy is estimated at 0.5-2.5% of the general population, depending on degree of consumption by age and geographic regions. The manifestations of shellfish allergy vary widely, but it tends to be more severe than most other food allergens. Tropomyosin is the major allergen and is responsible for cross-reactivity between members of the shellfish family, particularly among the crustacea. Newly described allergens and subtle differences in the structures of tropomyosin between different species of shellfish could account for the discrepancy between in vitro cross-antigenicity and clinical cross-allergenicity. The diagnosis requires a thorough medical history supported by skin testing or measurement of specific IgE level, and confirmed by appropriate oral challenge testing unless the reaction was life-threatening. Management of shellfish allergy is basically strict elimination, which in highly allergic subjects may include avoidance of touching or smelling and the availability of self-administered epinephrine. Specific immunotherapy is not currently available and requires the development of safe and effective protocols. PMID:22410209

  13. Fish and Shellfish Associated Disease Outbreaks.

    ERIC Educational Resources Information Center

    Levin, M.

    1978-01-01

    Presents a literature review of disease outbreaks related to fish and shellfish, covering publications of 1976-77. This review covers the chemical, bacterial, and viral diseases that are transmitted by fish and shellfish. A list of 50 references is also presented. (HM)

  14. 50 CFR Table 18 to Part 679 - Required Buying and Production Forms for use With State of Alaska Commercial Operator's Annual...

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... (E)Crab Buying (F) Crab Production Shrimp/Miscellaneous Shellfish (G)Shrimp/Misc.Shellfish Buying (G...) (H) Shrimp/Misc. Shellfish/Finfish Production Groundfish (I)(1) Groundfish Buying(I)(2) Groundfish...

  15. Detection of enteric viruses in shellfish

    USDA-ARS?s Scientific Manuscript database

    Norovirus and hepatitis A virus contamination are significant threats to the safety of shellfish and other foods. Methods for the extraction and assay of these viruses from shellfish are complex, time consuming, and technically challenging. Here, we itemize some of the salient points in extracting...

  16. An Outbreak of Norovirus Infection from Shellfish Soup Due to Unforeseen Insufficient Heating During Preparation.

    PubMed

    Lunestad, Bjørn Tore; Maage, Amund; Roiha, Irja Sunde; Myrmel, Mette; Svanevik, Cecilie Smith; Duinker, Arne

    2016-12-01

    Norovirus causes large outbreaks involving all age groups and are considered the most common cause of infectious foodborne diseases worldwide. The aim of this study was to describe a norovirus outbreak connected to insufficient heat treatment during preparation of a shellfish soup in serving portions, during a company Christmas celebration in Norway, December 2013. A questionnaire sent to the employees, showed that 67 % (n = 43) of the celebration participants, reported gastrointestinal symptoms including stomach pain, vomiting, diarrhoea and light fever in the period between 24 and 48 h post celebration. Several dishes were served, including shellfish soup made with carpet shell clams (Tapes rhomboides) in porcelain cups. Consuming this soup, was the only significant risk factor for infection. Norovirus GI and GII were detected in the remaining raw shellfish. To mimic the time and temperature obtained during bivalve soup preparation, raw chopped shellfish tissue and raw cepa onion were added in porcelain cups tempered to 20 °C. To each of these cups, boiling soup base was added. The temperature in the shellfish tissue was continuously recorded, and showed a maximum of 49 °C in the period between 3 and 7 min after adding the boiling soup base. After 1 h the temperature was 30 °C. This time and temperature combination was obviously not sufficient for inactivation of norovirus present in the shellfish tissue. In conclusion, the heat-absorbing capacity of cold ingredients, utensils and table wear porcelain should not be underestimated during food production. Consumers who want to avoid eating raw shellfish, should not assume that the shellfish tissue in preparation as described in our study is adequately heat treated.

  17. [Validation Study for Analytical Method of Diarrhetic Shellfish Poisons in 9 Kinds of Shellfish].

    PubMed

    Yamaguchi, Mizuka; Yamaguchi, Takahiro; Kakimoto, Kensaku; Nagayoshi, Haruna; Okihashi, Masahiro; Kajimura, Keiji

    2016-01-01

    A method was developed for the simultaneous determination of okadaic acid, dinophysistoxin-1 and dinophysistoxin-2 in shellfish using ultra performance liquid chromatography with tandem mass spectrometry. Shellfish poisons in spiked samples were extracted with methanol and 90% methanol, and were hydrolyzed with 2.5 mol/L sodium hydroxide solution. Purification was done on an HLB solid-phase extraction column. This method was validated in accordance with the notification of Ministry of Health, Labour and Welfare of Japan. As a result of the validation study in nine kinds of shellfish, the trueness, repeatability and within-laboratory reproducibility were 79-101%, less than 12 and 16%, respectively. The trueness and precision met the target values of notification.

  18. Harmful Algal Blooms and Public Health.

    PubMed

    Grattan, Lynn M; Holobaugh, Sailor; Morris, J Glenn

    2016-07-01

    The five most commonly recognized Harmful Algal Bloom related illnesses include Ciguatera poisoning, Paralytic Shellfish poisoning, Neurotoxin Shellfish poisoning, Diarrheic Shellfish Poisoning and Amnesic Shellfish poisoning. Although they are each the product of different toxins, toxin assemblages or HAB precursors these clinical syndromes have much in common. Exposure occurs through the consumption of fish or shellfish; routine clinical tests are not available for diagnosis; there is no known antidote for exposure; and the risk of these illnesses can negatively impact local fishing and tourism industries. Thus, illness prevention is of paramount importance to minimize human and public health risks. To accomplish this, close communication and collaboration is needed among HAB scientists, public health researchers and local, state and tribal health departments at academic, community outreach, and policy levels.

  19. New targets for expedient detection of viruses within shellfish

    USDA-ARS?s Scientific Manuscript database

    Previously our laboratory developed an expedient method for extraction of viral RNA from food-borne virus contaminated bivalve shellfish, termed the GPTT protocol. This protocol utilizes either whole shellfish or dissected digestive diverticula. This four step protocol utilizes a high pH glycine or...

  20. Paralytic shellfish toxins in phytoplankton and shellfish samples collected from the Bohai Sea, China.

    PubMed

    Liu, Yang; Yu, Ren-Cheng; Kong, Fan-Zhou; Chen, Zhen-Fan; Dai, Li; Gao, Yan; Zhang, Qing-Chun; Wang, Yun-Feng; Yan, Tian; Zhou, Ming-Jiang

    2017-02-15

    Phytoplankton and shellfish samples collected periodically from 5 representative mariculture zones around the Bohai Sea, Laishan (LS), Laizhou (LZ), Hangu (HG), Qinhuangdao (QHD) and Huludao (HLD), were analysed for paralytic shellfish toxins (PSTs) using an high-performance liquid chromatography (HPLC) method. Toxins were detected in 13 out of 20 phytoplankton samples, and N-sulfocarbamoyl toxins (C1/2) were predominant components of PSTs in phytoplankton samples with relatively low toxin content. However, two phytoplankton samples with high PST content collected from QHD and LS had unique toxin profiles characterized by high-potency carbamoyl toxins (GTX1/4) and decarbamoyl toxins (dcGTX2/3 and dcSTX), respectively. PSTs were commonly found in shellfish samples, and toxin content ranged from 0 to 27.6nmol/g. High level of PSTs were often found in scallops and clams. Shellfish from QHD in spring, and LZ and LS in autumn exhibited high risks of PST contamination. Copyright © 2016 Elsevier Ltd. All rights reserved.

  1. The importance of ATP-related compounds for the freshness and flavor of post-mortem fish and shellfish muscle: A review.

    PubMed

    Hong, Hui; Regenstein, Joe M; Luo, Yongkang

    2017-06-13

    ATP degradation is one of the most important biochemical changes in the post-mortem muscle of fish and shellfish. This process has long been recognized as an accurate way to evaluate freshness of fish and shellfish product. This review updates and condenses the overall history and recent advances in understanding the role of ATP-related compounds in post-mortem fish and shellfish muscle including a discussion of key analytical methods, their use as a freshness indicator, their roles in flavor enhancement, the factors affecting their transitions, and the possible mechanisms responsible for their impact on flavor and freshness. Moreover, some challenges and future directions for research regarding ATP-related compounds in fish and shellfish flavor and freshness are presented. With increasing consumer demands for fresh products with extended shelf life, understanding the relationships between ATP-related compounds and their involvement in the freshness and umami taste is a prerequisite for assuring the high quality of fish and shellfish.

  2. Evaluation of different conditions and culture media for the recovery of Aeromonas spp. from water and shellfish samples.

    PubMed

    Latif-Eugenín, F; Beaz-Hidalgo, R; Figueras, M J

    2016-09-01

    To perform a comparative study for determining the optimum culture method (direct plating or enrichment) and medium (ampicillin dextrin agar (ADA), starch ampicillin agar (SAA), bile salts irgasan brilliant green modified (BIBG-m)) for recovering Aeromonas species from water and shellfish samples. By direct culture, Aeromonas was detected in 65% (13/20) of the water samples and in 54·5% (6/11) of the shellfish samples. However, when a pre-enrichment step was included, the number of positive water samples increased to 75% (15/20) and the ones of shellfish to 90·1% (10/11). The enriched culture significantly favoured (P < 0·05) the isolation of Aeromonas allosaccharophila from water, Aeromonas salmonicida from shellfish and Aeromonas caviae from both types of samples. The most specific (P < 0·05) culture medium for detecting Aeromonas from water was ADA. However, no differences were observed in the case of shellfish samples (P > 0·05). Isolation of Aeromonas media from water was favoured (P < 0·05) in the ADA medium, while SAA enhanced (P < 0·05) the isolation of Aer. salmonicida from shellfish. The culture method and medium used influenced the recovery of some Aeromonas species from water and shellfish samples. This fact should be considered in future prevalence studies to avoid overestimating the above mentioned Aeromonas species. © 2016 The Society for Applied Microbiology.

  3. Fish and shellfish poisoning.

    PubMed

    Trevino, S

    1998-01-01

    To review history, biology, and medical aspects associated with fish and shellfish poisoning. Current literature, various Web sites, and Halstead's Volume II of Poisonous and Venomous Marine Animals. Determined by author. Determined by author. Fish and shellfish poisoning occur through the natural event of the food chain. Fish and shellfish consume algae that contain toxin-producing dinoflagellates. As a result they become contaminated and the toxin is concentrated as it moves up the food chain. Fish and shellfish can tolerate high levels of toxins, thereby appearing healthy while posing a significant danger to man. The toxin cannot be detected by sight, smell, or taste and is not destroyed by cooking or freezing. Thus man becomes an unsuspecting victim. There are several types of poisoning that occur through fish and shellfish consumption. They are ciguatera and scombroid fish poisoning; and paralytic, diarrheic, neurotoxic, and amnesic shellfish poisoning. A different toxin produces each of these poisonings; however, sources and symptoms may be similar among these poisonings making them difficult to diagnose. These intoxications can vary in severity from mild to fatal depending on the type and amount of toxin ingested. Age and underlying illnesses may also contribute to the outcome of these poisonings. Though people are aware of health warnings and may think they are important, it is human nature to think that "this could not possibly happen to me". Therefore, they fail to make the necessary changes required to reduce the incidence of fish and shellfish poisoning.

  4. 76 FR 37815 - Cooperative Agreement To Support Shellfish Safety Assistance Project

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-28

    ...; funding support to research the influence of water and air temperature, dissolved oxygen, and nutrients on... controls to reduce the risk of illness associated with molluscan shellfish consumption, including Vibrio... professionals concerning Vibrio illness and shellfish consumption; 9. Development and maintenance of a World...

  5. 27 CFR 5.32a - Voluntary disclosure of major food allergens.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ..., or cod), Crustacean shellfish (for example, crab, lobster, or shrimp), tree nuts (for example... (for example, almonds, pecans, or walnuts); (ii) In the case of Crustacean shellfish, it means the name of the species of Crustacean shellfish (for example, crab, lobster, or shrimp); and (iii) The names...

  6. 27 CFR 5.32a - Voluntary disclosure of major food allergens.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ..., or cod), Crustacean shellfish (for example, crab, lobster, or shrimp), tree nuts (for example... (for example, almonds, pecans, or walnuts); (ii) In the case of Crustacean shellfish, it means the name of the species of Crustacean shellfish (for example, crab, lobster, or shrimp); and (iii) The names...

  7. 27 CFR 7.22a - Voluntary disclosure of major food allergens.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ..., or cod), Crustacean shellfish (for example, crab, lobster, or shrimp), tree nuts (for example... (for example, almonds, pecans, or walnuts); and (ii) In the case of Crustacean shellfish, it means the name of the species of Crustacean shellfish (for example, crab, lobster, or shrimp); and (iii) The...

  8. 27 CFR 7.22a - Voluntary disclosure of major food allergens.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ..., or cod), Crustacean shellfish (for example, crab, lobster, or shrimp), tree nuts (for example... (for example, almonds, pecans, or walnuts); and (ii) In the case of Crustacean shellfish, it means the name of the species of Crustacean shellfish (for example, crab, lobster, or shrimp); and (iii) The...

  9. 27 CFR 7.22a - Voluntary disclosure of major food allergens.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ..., or cod), Crustacean shellfish (for example, crab, lobster, or shrimp), tree nuts (for example... (for example, almonds, pecans, or walnuts); and (ii) In the case of Crustacean shellfish, it means the name of the species of Crustacean shellfish (for example, crab, lobster, or shrimp); and (iii) The...

  10. 27 CFR 5.32a - Voluntary disclosure of major food allergens.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ..., or cod), Crustacean shellfish (for example, crab, lobster, or shrimp), tree nuts (for example... (for example, almonds, pecans, or walnuts); (ii) In the case of Crustacean shellfish, it means the name of the species of Crustacean shellfish (for example, crab, lobster, or shrimp); and (iii) The names...

  11. 21 CFR 123.28 - Source controls.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 2 2013-04-01 2013-04-01 false Source controls. 123.28 Section 123.28 Food and... CONSUMPTION FISH AND FISHERY PRODUCTS Raw Molluscan Shellfish § 123.28 Source controls. (a) In order to meet... molluscan shellfish harvested from growing waters approved for harvesting by a shellfish control authority...

  12. 21 CFR 123.28 - Source controls.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 2 2011-04-01 2011-04-01 false Source controls. 123.28 Section 123.28 Food and... CONSUMPTION FISH AND FISHERY PRODUCTS Raw Molluscan Shellfish § 123.28 Source controls. (a) In order to meet... molluscan shellfish harvested from growing waters approved for harvesting by a shellfish control authority...

  13. 21 CFR 123.28 - Source controls.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 2 2012-04-01 2012-04-01 false Source controls. 123.28 Section 123.28 Food and... CONSUMPTION FISH AND FISHERY PRODUCTS Raw Molluscan Shellfish § 123.28 Source controls. (a) In order to meet... molluscan shellfish harvested from growing waters approved for harvesting by a shellfish control authority...

  14. 21 CFR 123.28 - Source controls.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 2 2014-04-01 2014-04-01 false Source controls. 123.28 Section 123.28 Food and... CONSUMPTION FISH AND FISHERY PRODUCTS Raw Molluscan Shellfish § 123.28 Source controls. (a) In order to meet... molluscan shellfish harvested from growing waters approved for harvesting by a shellfish control authority...

  15. Harmful Algal Blooms and Public Health

    PubMed Central

    Grattan, Lynn M.; Holobaugh, Sailor; Morris, J. Glenn

    2015-01-01

    The five most commonly recognized Harmful Algal Bloom related illnesses include Ciguatera poisoning, Paralytic Shellfish poisoning, Neurotoxin Shellfish poisoning, Diarrheic Shellfish Poisoning and Amnesic Shellfish poisoning. Although they are each the product of different toxins, toxin assemblages or HAB precursors these clinical syndromes have much in common. Exposure occurs through the consumption of fish or shellfish; routine clinical tests are not available for diagnosis; there is no known antidote for exposure; and the risk of these illnesses can negatively impact local fishing and tourism industries. Thus, illness prevention is of paramount importance to minimize human and public health risks. To accomplish this, close communication and collaboration is needed among HAB scientists, public health researchers and local, state and tribal health departments at academic, community outreach, and policy levels. PMID:27616971

  16. National collaborative shellfish pollution-indicator study: Site selection. Phase 1. Rept. for 1987-88

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Leonard, D.L.; Broutman, M.A.; Caverly, K.E.

    1988-07-01

    Each year approximately 16 million acres of estuarine waters are classified for the harvest of molluscan shellfish as open or limited to harvest according to microbiological 'indicator' standards and pollution survey guidelines established by the National Shellfish Sanitation Program. The program was developed in the 1920's in response to typhoid fever outbreaks and may no longer protect the consumer from the most prevalent shellfish-borne diseases: hepatitis and gastroenteritis. Today, 1/3 of productive or potentially productive shellfish-growing waters are closed to harvest at some time during the year. In response to these problems, the industry has initiated a national cooperative effortmore » to re-evaluate the standard and establish a classification system directly related to public health implications.« less

  17. [Nutritive value of shellfish consumed in Chile].

    PubMed

    Pak, N; Vera, G; Araya, H

    1985-03-01

    The purpose of the present study was to determine the protein quality and digestibility of shellfish commonly consumed in Chile, and to estimate its contribution to the protein needs of the Chilean population. The shellfish studied were chorito (Mytilus edulis chilensis), macha (Mesodesma donacium), loco (Concholepas concholepas), cholga (Aulacomya ater), erizo (Loxechinus albus) and almeja (no specific variety). The NPU method was used to determine protein quality. The percentage of protein adequacy for adult rations was calculated according to FAO/WHO 1973. The contribution of shellfish to the protein availability according to the family income of the Santiago population, was also calculated. Most of the shellfish presented NPU values of about 70; the lowest values were found for loco (54.9) and macha (63.3). The apparent and true digestibility gave an average of 83.6 and 90.4, respectively. The percentage of protein adequacy of habitual rations ranged between 27% (erizo) and 58% (loco). The availability of shellfish protein in relation to total protein increased from 0.4 to 2.5% when income increased. It is concluded therefore, that shellfish protein is, in general, of good quality. Nevertheless, it might be considered of poor influence insofar as fulfilling the protein needs of the population studied, whatever its socioeconomic level.

  18. Density of total and pathogenic (tdh+) Vibrio parahaemolyticus in Atlantic and Gulf coast molluscan shellfish at harvest.

    PubMed

    Cook, David W; Bowers, John C; DePaola, Angelo

    2002-12-01

    The densities of total and pathogenic Vibrio parahaemolyticus in 671 samples of molluscan shellfish harvested in 1999 and 2000 from 14 sites in seven Gulf and Atlantic coast states were determined at 2-week intervals over a period of 12 to 16 months in each state. Changes in V. parahaemolyticus densities in shellfish between harvest and sample analysis were minimized with time and temperature controls. Densities were measured by direct plating techniques, and gene probes were used for identification. Total and pathogenic V. parahaemolyticus organisms were identified with probes for the thermolabile direct hemolysin (tlh) gene and the thermostable direct hemolysin (tdh) gene, respectively. An enrichment procedure involving 25 g of shellfish was also used for the recovery of pathogenic V. parahaemolyticus. The densities of V. parahaemolyticus in shellfish from all harvest sites were positively correlated with water temperature. Shellfish from the Gulf Coast typically had higher densities of V. parahaemolyticus than did shellfish harvested from the North Atlantic or mid-Atlantic coast. Vibrio parahaemolyticus counts exceeded 1,000 CFU/g for only 5% of all samples. Pathogenic (tdh+) V. parahaemolyticus was detected in approximately 6% of all samples by both procedures, and 61.5% of populations in the positive samples from the direct plating procedure were at the lower limit of detection (10 CFU/g). The frequency of detection of pathogenic V. parahaemolyticus was significantly related to water temperature and to the density of total V. parahaemolyticus. The failure to detect pathogenic V. parahaemolyticus in shellfish more frequently was attributed to the low numbers and uneven distribution of the organism.

  19. 75 FR 76351 - Magnuson-Stevens Fishery Conservation and Management Act Provisions; Fisheries of the...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-08

    ... that accumulate in water column filter- feeding shellfish. Shellfish contaminated with the toxin, if... to collect water and shellfish samples from Federal waters off of southern New England. The FDA, in... ocean quahogs for human consumption. The FDA indicated that testing of clams from the portion of the GB...

  20. 40 CFR 130.7 - Total maximum daily loads (TMDL) and individual water quality-based effluent limitations.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... of a balanced indigenous population of shellfish, fish and wildlife. (3) For the purposes of listing... propagation of a balanced, indigenous population of shellfish, fish and wildlife. Such estimates shall take... water quality criteria for protection and propagation of a balanced, indigenous population of shellfish...

  1. 40 CFR 130.7 - Total maximum daily loads (TMDL) and individual water quality-based effluent limitations.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... of a balanced indigenous population of shellfish, fish and wildlife. (3) For the purposes of listing... propagation of a balanced, indigenous population of shellfish, fish and wildlife. Such estimates shall take... water quality criteria for protection and propagation of a balanced, indigenous population of shellfish...

  2. 40 CFR 130.7 - Total maximum daily loads (TMDL) and individual water quality-based effluent limitations.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... of a balanced indigenous population of shellfish, fish and wildlife. (3) For the purposes of listing... propagation of a balanced, indigenous population of shellfish, fish and wildlife. Such estimates shall take... water quality criteria for protection and propagation of a balanced, indigenous population of shellfish...

  3. Fish and shellfish allergy.

    PubMed

    Thalayasingam, Meera; Lee, Bee-Wah

    2015-01-01

    Fish and shellfish consumption has increased worldwide, and there are increasing reports of adverse reactions to fish and shellfish, with an approximate prevalence of 0.5-5%. Fish allergy often develops early in life, whilst shellfish allergy tends to develop later, from adolescence onwards. Little is known about the natural history of these allergies, but both are thought to be persistent. The clinical manifestations of shellfish allergy, in particular, may vary from local to life-threatening 'anaphylactic' reactions within an individual and between individuals. Parvalbumin and tropomyosin are the two major allergens, but several other allergens have been cloned and described. These allergens are highly heat and biochemically stable, and this may in part explain the persistence of these allergies. Diagnosis requires a thorough history, skin prick and in-vitro-specific IgE tests, and oral challenges may be needed for diagnostic confirmation. Strict avoidance of these allergens is the current standard of clinical care for allergic patients, and when indicated, an anaphylactic plan with an adrenaline auto-injector is prescribed. There are no published clinical trials evaluating specific oral immunotherapy for fish or shellfish allergy. © 2015 S. Karger AG, Basel.

  4. Phycotoxins in Marine Shellfish: Origin, Occurrence and Effects on Humans.

    PubMed

    Farabegoli, Federica; Blanco, Lucía; Rodríguez, Laura P; Vieites, Juan Manuel; Cabado, Ana García

    2018-05-29

    Massive phytoplankton proliferation, and the consequent release of toxic metabolites, can be responsible for seafood poisoning outbreaks: filter-feeding mollusks, such as shellfish, mussels, oysters or clams, can accumulate these toxins throughout the food chain and present a threat for consumers' health. Particular environmental and climatic conditions favor this natural phenomenon, called harmful algal blooms (HABs); the phytoplankton species mostly involved in these toxic events are dinoflagellates or diatoms belonging to the genera Alexandrium , Gymnodinium , Dinophysis , and Pseudo-nitzschia . Substantial economic losses ensue after HABs occurrence: the sectors mainly affected include commercial fisheries, tourism, recreational activities, and public health monitoring and management. A wide range of symptoms, from digestive to nervous, are associated to human intoxication by biotoxins, characterizing different and specific syndromes, called paralytic shellfish poisoning, amnesic shellfish poisoning, diarrhetic shellfish poisoning, and neurotoxic shellfish poisoning. This review provides a complete and updated survey of phycotoxins usually found in marine invertebrate organisms and their relevant properties, gathering information about the origin, the species where they were found, as well as their mechanism of action and main effects on humans.

  5. A highly rapid and simple competitive enzyme-linked immunosorbent assay for monitoring paralytic shellfish poisoning toxins in shellfish.

    PubMed

    Kawatsu, Kentaro; Kanki, Masashi; Harada, Tetsuya; Kumeda, Yuko

    2014-11-01

    Using a streptavidin-coated well plate, a biotin-labelled anti-gonyautoxin 2/3 monoclonal antibody GT-13A, and a decarbamoyl saxitoxin-peroxidase conjugate, a direct competitive enzyme-linked immunosorbent assay (PSP-ELISA) was developed for monitoring paralytic shellfish poisoning (PSP) toxins in shellfish. This assay is simple to perform and can be completed in approximately 20 min. The PSP-ELISA was compared to the mouse bioassay (MBA) for the detection of PSP toxins in shellfish samples (n=83) collected from the coast of Osaka Prefecture, Japan. When positive and negative results were indicated based on the regulatory limit for PSP toxins (4 mouse unit(MU)/g of shellfish meat), the PSP-ELISA results showed a sensitivity of 100% (25 of 25) and a specificity of 89.7% (52 of 58 samples) compared to the MBA results. These results suggest that the PSP-ELISA could be used as a rapid and simple screening method prior to the MBA. Copyright © 2014 Elsevier Ltd. All rights reserved.

  6. Most-probable-number loop-mediated isothermal amplification-based procedure enhanced with K antigen-specific immunomagnetic separation for quantifying tdh(+) Vibrio parahaemolyticus in molluscan Shellfish.

    PubMed

    Tanaka, Natsuko; Iwade, Yoshito; Yamazaki, Wataru; Gondaira, Fumio; Vuddhakul, Varaporn; Nakaguchi, Yoshitsugu; Nishibuchi, Mitsuaki

    2014-07-01

    Although thermostable direct hemolysin-producing (tdh(+)) Vibrio parahaemolyticus is the leading cause of seafood-borne gastroenteritis, the enumeration of tdh(+) V. parahaemolyticus remains challenging due to its low densities in the environment. In this study, we developed a most-probable-number (MPN)-based procedure designated A-IS(1)-LAMP, in which an immunomagnetic separation (IMS) technique targeting as many as 69 established K antigens and a loop-mediated isothermal amplification (LAMP) assay targeting the thermostable direct hemolysin (tdh) gene were applied in an MPN format. Our IMS employed PickPen, an eight-channel intrasolution magnetic particle separation device, which enabled a straightforward microtiter plate-based IMS procedure (designated as PickPen-IMS). The ability of the procedure to quantify a wide range of tdh(+) V. parahaemolyticus levels was evaluated by testing shellfish samples in Japan and southern Thailand, where shellfish products are known to contain relatively low and high levels of total V. parahaemolyticus, respectively. The Japanese and Thai shellfish samples showed, respectively, relatively low (< 3 to 11 MPN/10 g) and considerably higher (930 to 110,000 MPN/10 g) levels of tdh(+) V. parahaemolyticus, raising concern about the safety of Thai shellfish products sold to domestic consumers at local morning markets. LAMP showed similar or higher performance than conventional PCR in the detection and quantification of a wide range of tdh(+) V. parahaemolyticus levels in shellfish products. Whereas a positive effect of PickPen-IMS was not observed in MPN determination, PickPen-IMS was able to concentrate tdh(+) V. parahaemolyticus 32-fold on average from the Japanese shellfish samples at an individual tube level, suggesting a possibility of using PickPen-IMS as an optional tool for specific shellfish samples. The A-IS(1)-LAMP procedure can be used by any health authority in the world to measure the tdh(+) V. parahaemolyticus levels in shellfish products.

  7. Assessing the presence of marine toxins in bivalve molluscs from southwest India.

    PubMed

    Turner, Andrew D; Dhanji-Rapkova, Monika; Rowland-Pilgrim, Stephanie; Turner, Lucy M; Rai, Ashwin; Venugopal, Moleyur N; Karunasagar, Indrani; Godhe, Anna

    2017-12-15

    The south west coast of India has been showing a steady increase in shellfish cultivation both for local consumption and fishery export, over recent years. Perna viridis and Crassostrea madrasensis are two species of bivalve molluscs which grow in some selected regions of southern Karnataka, close to the city of Mangalore. In the early 1980s, shellfish consumers in the region were affected by intoxication from Paralytic Shellfish Poison present in local bivalves (clams and oysters) resulting in hospitalisation of many, including one fatality. Since then, there have been no further reports of serious shellfish intoxication and there is little awareness of the risks from natural toxins and no routine monitoring programme in place to protect shellfish consumers. This study presents the findings from the first ever systematic assessment of the presence of marine toxins in mussels and oysters grown in four different shellfish harvesting areas in the region. Shellfish were collected and subjected to analysis for ASP, PSP and lipophilic toxins, as well as a suite of non-EU regulated toxins such as tetrodotoxin and selected cyclic imines. Results revealed the presence of low levels of PSP toxins in oysters throughout the study period. Overall, total toxicities reached a maximum of 10% of the EU regulatory limit of 800 μg STX eq/kg. Toxin profiles were similar to those reported from the 1980 outbreak. No evidence was found for significant levels of ASP and lipophilic toxins, although some cyclic imines were detected, including gymnodimine. The results indicated that the risk to shellfish consumers during this specific study period would have been low. However, with historical evidence for extremely high levels of PSP toxins in molluscs, there is a strong need for routine surveillance of shellfish production areas for marine toxins, in order to mitigate against human health impacts resulting from unexpected harmful algal blooms, with potentially devastating socio-economic consequences. Copyright © 2017 Elsevier Ltd. All rights reserved.

  8. Intoxications from the seas: ciguatera, scombroid, and paralytic shellfish poisoning.

    PubMed

    Sanders, W E

    1987-09-01

    Sporadic cases and outbreaks of intoxications borne by fish and shellfish have increased in frequency during recent years. Ciguatera, scombroid, and paralytic shellfish poisoning account for nearly 16 per cent of all reported foodborne outbreaks of disease in the United States. Fishborne ciguatera and paralytic shellfish poisoning are characterized by gastrointestinal and neuromuscular manifestations attributable to toxins of dinoflagellates. These toxins impair sodium transport in cell membranes. Treatment is primarily supportive. Scombroid fish intoxication resembles histamine poisoning and may be treated effectively with antihistamines or cimetidine. Prevention of these intoxications at present depends upon avoidance of potential vectors.

  9. Attitudinal Factors and Personal Characteristics Influence Support for Shellfish Aquaculture in Rhode Island (US) Coastal Waters.

    PubMed

    Dalton, Tracey M; Jin, Di

    2018-05-01

    This study explores public interests associated with shellfish aquaculture development in coastal waters of Rhode Island (US). Specifically, we examine (1) the levels of public support for (or opposition to) shellfish aquaculture development and (2) factors driving the levels of support, using survey data and ordinal logistic regressions. Results of the analysis identify several key attitudinal factors affecting individual's support for shellfish aquaculture in Rhode Island (RI). The level of support is positively associated with attitudes related to shellfish aquaculture's benefits to the local economy and its role as a nutritional food option, and negatively influenced by attitudes related to aquaculture farms' effects on aesthetic quality and their interference with other uses. Findings highlight that support for (or opposition to) aquaculture in RI is driven more by attitudes associated with social impacts than by those associated with environmental impacts. The level of support is also affected by personal characteristics related to an individual's participation in recreational activities. For instance, bicycle riders tend to be supportive of shellfish aquaculture while respondents who participate in sailing and birding are less supportive. By identifying the broader public's interests in shellfish aquaculture, findings from this study and others like it can be used to address public concerns, incorporate public perceptions and attitudes into permitting decisions, and develop outreach targeted at specific stakeholder groups.

  10. Surveillance of hepatitis E virus contamination in shellfish in China.

    PubMed

    Gao, Shenyang; Li, Dandan; Zha, Enhui; Zhou, Tiezhong; Wang, Shen; Yue, Xiqing

    2015-02-11

    Hepatitis E virus (HEV) has been confirmed to be a zoonotic virus of worldwide distribution. HEV contamination in the water environment has not been well examined in China. The objective of this study was to evaluate HEV contamination in shellfish in a coastal area of China. Such contamination would be significant for evaluating public health risks. samples of three species shellfish were collected from thirteen points of estuarine tidal flats around the Bohai Gulf and screened for HEV RNA using an in-house nested RT-PCR assay. The detected HEV-positive samples were further verified by gene cloning and sequencing analysis. the overall HEV-positive detection rate is approximately 17.5% per kilogram of shellfish.  HEV was more common among S. subcrenata (28.2%), followed by A. granosa (14.3%) and R. philippinarum (11.5%). The phylogenetic analysis of the 13 HEV strains detected revealed that gene fragments fell into two known 4 sub-genotypes (4b/4d) groups and another unknown group. 13 different sub-genotype 4 HEVs were found in contaminated shellfish in the Bohai Gulf rim. The findings suggest that a health risk may exist for users of waters in the Bonhai area and to consumers of shellfish.  Further research is needed to assess the sources and infectivity of HEV in these settings, and to evaluate additional shellfish harvesting areas.

  11. Screening tests for the rapid detection of diarrhetic shellfish toxins in Washington State.

    PubMed

    Eberhart, Bich-Thuy L; Moore, Leslie K; Harrington, Neil; Adams, Nicolaus G; Borchert, Jerry; Trainer, Vera L

    2013-09-30

    The illness of three people due to diarrhetic shellfish poisoning (DSP) following their ingestion of recreationally harvested mussels from Sequim Bay State Park in the summer of 2011, resulted in intensified monitoring for diarrhetic shellfish toxins (DSTs) in Washington State. Rapid testing at remote sites was proposed as a means to provide early warning of DST events in order to protect human health and allow growers to test "pre-harvest" shellfish samples, thereby preventing harvest of toxic product that would later be destroyed or recalled. Tissue homogenates from several shellfish species collected from two sites in Sequim Bay, WA in the summer 2012, as well as other sites throughout Puget Sound, were analyzed using three rapid screening methods: a lateral flow antibody-based test strip (Jellett Rapid Test), an enzyme-linked immunosorbent assay (ELISA) and a protein phosphatase 2A inhibition assay (PP2A). The results were compared to the standard regulatory method of liquid chromatography coupled with tandem mass spectroscopy (LC-MS/MS). The Jellett Rapid Test for DSP gave an unacceptable number of false negatives due to incomplete extraction of DSTs using the manufacturer's recommended method while the ELISA antibody had low cross-reactivity with dinophysistoxin-1, the major toxin isomer in shellfish from the region. The PP2A test showed the greatest promise as a screening tool for Washington State shellfish harvesters.

  12. Screening Tests for the Rapid Detection of Diarrhetic Shellfish Toxins in Washington State

    PubMed Central

    Eberhart, Bich-Thuy L.; Moore, Leslie K.; Harrington, Neil; Adams, Nicolaus G.; Borchert, Jerry; Trainer, Vera L.

    2013-01-01

    The illness of three people due to diarrhetic shellfish poisoning (DSP) following their ingestion of recreationally harvested mussels from Sequim Bay State Park in the summer of 2011, resulted in intensified monitoring for diarrhetic shellfish toxins (DSTs) in Washington State. Rapid testing at remote sites was proposed as a means to provide early warning of DST events in order to protect human health and allow growers to test “pre-harvest” shellfish samples, thereby preventing harvest of toxic product that would later be destroyed or recalled. Tissue homogenates from several shellfish species collected from two sites in Sequim Bay, WA in the summer 2012, as well as other sites throughout Puget Sound, were analyzed using three rapid screening methods: a lateral flow antibody-based test strip (Jellett Rapid Test), an enzyme-linked immunosorbent assay (ELISA) and a protein phosphatase 2A inhibition assay (PP2A). The results were compared to the standard regulatory method of liquid chromatography coupled with tandem mass spectroscopy (LC-MS/MS). The Jellett Rapid Test for DSP gave an unacceptable number of false negatives due to incomplete extraction of DSTs using the manufacturer’s recommended method while the ELISA antibody had low cross-reactivity with dinophysistoxin-1, the major toxin isomer in shellfish from the region. The PP2A test showed the greatest promise as a screening tool for Washington State shellfish harvesters. PMID:24084788

  13. Relevance of F-Specific RNA Bacteriophages in Assessing Human Norovirus Risk in Shellfish and Environmental Waters

    PubMed Central

    Hartard, C.; Banas, S.; Loutreul, J.; Rincé, A.; Benoit, F.; Boudaud, N.

    2016-01-01

    ABSTRACT Human noroviruses (HuNoVs) are the main cause of shellfish-borne gastroenteritis outbreaks. In the absence of routine technical approaches allowing infectious particles to be detected, this viral pathogen is currently targeted by genome research, leading to difficult interpretations. In this study, we investigated the potential of F-specific RNA bacteriophages (FRNAPH) as fecal and viral contamination indicators in shellfish and water from a local harvesting area. FRNAPH were also used as microbial source tracking tools. Constraints imposed by detection limits are illustrated here by the detection of infectious FRNAPH in several samples in the absence of FRNAPH genomes. The opposite situation was also observed, likely explained by the persistence of the genomes being greater than infectivity. Similar considerations may be applied to HuNoVs, suggesting that HuNoV genome targeting is of limited relevance in assessing infectious risks. While FRNAPH did not provide any benefits compared to Escherichia coli as fecal pollution indicators in water, novel observations were made in shellfish: contrary to E. coli, a seasonal trend of infectious FRNAPH concentrations was observed. These concentrations were higher than those found in water, confirming bioaccumulation in shellfish. This study also underlines a relationship between the presence of HuNoV genomes and those of human-specific FRNAPH subgroup II (FRNAPH-II) in shellfish collected throughout Europe. Further research should be undertaken to evaluate FRNAPH potential as an indicator of the presence of infectious HuNoVs. To this end, shellfish involved in HuNoV-caused gastroenteritis outbreaks should be analyzed for the presence of infectious FRNAPH-II. IMPORTANCE This work provides new data about the use of F-specific RNA phages (FRNAPH) as a tool for evaluating fecal or viral contamination, especially in shellfish. In our case study, FRNAPH did not provide any benefits compared to E. coli as fecal pollution indicators in water but were found to be very useful in shellfish. Their concentrations in shellfish were higher than those found in the surrounding water, confirming bioaccumulation. This study also underlines a relationship between the presence of human norovirus genomes (HuNoVs) and those of FRNAPH subgroup II (FRNAPH-II). Considering that the two virus types have similar behaviors and since FRNAPH infectivity can be investigated, the specific detection of infectious FRNAPH-II could be regarded as an indication of the presence of infectious HuNoVs. The contribution of infectious human FRNAPH targeting for assessing the viral risk associated with HuNoVs in shellfish should thus be investigated. PMID:27422833

  14. Food protein-induced enterocolitis syndrome caused by fish and/or shellfish in Italy.

    PubMed

    Miceli Sopo, Stefano; Monaco, Serena; Badina, Laura; Barni, Simona; Longo, Giorgio; Novembre, Elio; Viola, Serena; Monti, Giovanna

    2015-12-01

    The study describes the demographic features, culprit foods, clinical features and outcomes for children presenting with acute fish and/or shellfish food protein-induced enterocolitis syndrome (FPIES) in four Italian paediatric allergy centres. A retrospective/prospective study was undertaken. All children diagnosed with fish or shellfish FPIES were enrolled. The diagnosis of FPIES was based on Sicherer's or Miceli Sopo clinical criteria. Skin prick tests (SPT) were performed in all patients, at the time of diagnosis and prior to OFC. Seventy children were enrolled. Mean age at first episode was 14 months (range 6-46 months); mean age at diagnosis was 34 months (range 6-164 months). Sole and cod were the fish most commonly implicated. Fifty-seven of 70 (81%) children had FPIES exclusively to fish, 37 of 57 (65%) children had single-fish FPIES, 20 of 57 (35%) multiple-fish FPIES, nine of 70 (13%) presented adverse reactions exclusively to shellfish, and four of 70 (6%) presented adverse reactions to both fish and shellfish. Only four (5.7%) children presented episodes of acute FPIES with different foods (2 to cow's milk, 1 to egg, 1 to beef); in all cases, onset was prior to that of fish or shellfish FPIES. Fifteen of 70 (21%) children tolerated fish other than the offending fish. Twenty-four of 70 (34%) children achieved tolerance (age range 24-102 months). The chief peculiarities of acute fish and shellfish FPIES, compared to more frequent cow's milk or soy FPIES, are (i) later age of onset, (ii) longer persistence and (iii) possibility of tolerating fish other than the offending fish. Adverse reactions with shellfish are possible. © 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  15. A model for estimating pathogen variability in shellfish and predicting minimum depuration times.

    PubMed

    McMenemy, Paul; Kleczkowski, Adam; Lees, David N; Lowther, James; Taylor, Nick

    2018-01-01

    Norovirus is a major cause of viral gastroenteritis, with shellfish consumption being identified as one potential norovirus entry point into the human population. Minimising shellfish norovirus levels is therefore important for both the consumer's protection and the shellfish industry's reputation. One method used to reduce microbiological risks in shellfish is depuration; however, this process also presents additional costs to industry. Providing a mechanism to estimate norovirus levels during depuration would therefore be useful to stakeholders. This paper presents a mathematical model of the depuration process and its impact on norovirus levels found in shellfish. Two fundamental stages of norovirus depuration are considered: (i) the initial distribution of norovirus loads within a shellfish population and (ii) the way in which the initial norovirus loads evolve during depuration. Realistic assumptions are made about the dynamics of norovirus during depuration, and mathematical descriptions of both stages are derived and combined into a single model. Parameters to describe the depuration effect and norovirus load values are derived from existing norovirus data obtained from U.K. harvest sites. However, obtaining population estimates of norovirus variability is time-consuming and expensive; this model addresses the issue by assuming a 'worst case scenario' for variability of pathogens, which is independent of mean pathogen levels. The model is then used to predict minimum depuration times required to achieve norovirus levels which fall within possible risk management levels, as well as predictions of minimum depuration times for other water-borne pathogens found in shellfish. Times for Escherichia coli predicted by the model all fall within the minimum 42 hours required for class B harvest sites, whereas minimum depuration times for norovirus and FRNA+ bacteriophage are substantially longer. Thus this study provides relevant information and tools to assist norovirus risk managers with future control strategies.

  16. [Distribution of thermostable direct hemolysin (TDH)- and TDH-related hemolysin (TRH)-producing Vibrio parahaemolyticus in coastal Shimane Prefecture and TDH and TRH V parahaemolyticus contamination of retail shellfish].

    PubMed

    Fukushima, Hiroshi

    2007-03-01

    We studied distribution of thermostable direct hemolysin (TDH)- and TDH-related hemolysin (TRH)-producing Vibrio parahaemolyticus in coastal sea water, sediment, and shellfish and related retail shellfish contamination in Shimane Prefecture, Japan, between 2002 and 2004. V. parahaemolyticus was isolated from > 80% of sea water, sediment, and shellfish. The detection of TDH gene (tdh) and TRH gene (trh)-positive V parahaemolyticus in sea water was 11%, in sediment 16%, and in shellfish 26%. The number of genes and gene-related in seawater was 23 MPN/L, in sediment 29 MPN/100 g, and in shellfish 460 MPN/10 g. TDH- and TRH-producing V. parahaemolyticus detected in seawater was 5%, in sediment 11% and in shellfish 14%. The continuous distribution of TDH-producing O2:K28, O4:K88, O4:K37, and O4:KUT organisms on the western coast and TRH2-producing O5:k30, O5:K43, O10:K19, O10:KUT, O11:K40, O11:KUT, and OUT:KUT organisms on the Oki Island coast suggested the settlement of these organisms in these coastal environments. From 7 (12%) of 59 retail short-necked clam samples, we isolated TDH-producing O 1:KUT, O3:K6 (2 strains from 2 samples imported from Korea), O4:K12, OUT:K8, and TRH2-producing OUT:K40 and OUT:K51 organisms. These findings suggested that TDH- and TRH-producing V. parahaemolyticus are widely distributed along the coast of this prefecture and are transported by contaminated retail shellfish from other areas.

  17. [Accumulation and degradation of organochorine pesticides in shellfish culture environment in Xiamen sea area].

    PubMed

    Zhong, Shuo-liang; Dong, Li-ming

    2011-09-01

    By using GC-ECD, the concentrations of organochlorine pesticides hexachlorocyclohexane (HCH) and dichlorodiphenyltrichloroethane (DDT) in the shellfish culture environment (sea water, sediments, and culture-shellfishes) in Xiamen sea area were analyzed, and the accumulation and degradation patterns of the HCH and DDT were preliminarily approached. In the sea area, there existed remarkable differences in the accumulation and degradation of HCH and DDT among different shellfish culture environments, being mostly associated with the habitation environment and physiological life habits of shellfish. The accumulated HCH isomers (Rx > 1) were mainly beta-HCH, delta-HCH, and gamma-HCH, whereas the degraded HCH isomers (Rx < 1) were mainly alpha-HCH. The ratio of alpha-HCH to gamma-HCH was less than or equal to 1.0, suggesting that the HCH was come from industrial HCH and lindane, most of the HCH had remained in the culture environment for a longer time, and a small amount of lindane was imported. The DDT in the sea water was aerobically degraded, its main degradation product was DDE, and the ratios of (DDD+DDE) to DDTs (p,p-DDE+p,p-DDD+o,p-DDT+p,p-DDT) was less than 0.5, whereas the DDT in sediments and shellfishes was anaerobically degraded, its main degradation product was DDD, and the ratios of (DDD+DDE) to DDTs was greater than 0.5, suggesting that there was a small amount of DDT newly imported in the sea water, and most DDT in sediments and shellfishes were already degraded and transformed into DDD and DDE. There were definite differences in the degradation rates of HCH isomers in the culture environment, suggesting the conformational change of HCH in its transformation processes in the shellfish culture ecosystem.

  18. Public health assessment for USMC Marine Corps Recruit Depot (a/k/a Parris Island Marine Corps Recruit Depot), Parris Island, Beaufort County, South Carolina, Region 4. Cerclis No. SC6170022762

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    NONE

    1996-09-12

    Marine Corps Recruit Depot, (MCRD) Parris Island was proposed for listing on U.S. Environmental Protection Agency`s National Priorities List in August 1994. We identified two areas where chemical contaminants in soil entered the surface water wetland areas and bioaccumulated in edible fish and shellfish species: (1) contaminated fish and shellfish at the Causeway Landfill (Site 3) and (2) contaminated shellfish near the Rifle Range. These exposure situations pose no apparent public health hazard due to the low levels of contaminants detected in fish and shellfish. However, because the landfill has no impermeable cap nor leachate collection system, it is notmore » known whether contaminants in fish and shellfish will increase over time. The remaining 57 contaminated areas pose no public health hazard because people are not coming in contact with contaminants.« less

  19. Complete genome sequence for the shellfish pathogen Vibrio coralliilyticus RE98 isolated from a shellfish hatchery

    USDA-ARS?s Scientific Manuscript database

    Vibrio coralliilyticus is a pathogen of corals and larval shellfish. Publications on strain RE98 list it as a Vibrio tubiashii; however, whole genome sequencing confirms RE98 as V. coralliilyticus containing a total of 6,037,824 bp consisting of two chromosomes (3,420,228 and 1,917,482 bp), and two...

  20. Cytotoxicity and mycotoxin production of shellfish-derived Penicillium spp., a risk for shellfish consumers.

    PubMed

    Geiger, M; Guitton, Y; Vansteelandt, M; Kerzaon, I; Blanchet, E; Robiou du Pont, T; Frisvad, J C; Hess, P; Pouchus, Y F; Grovel, O

    2013-11-01

    In order to assess the putative toxigenic risk associated with the presence of fungal strains in shellfish-farming areas, Penicillium strains were isolated from bivalve molluscs and from the surrounding environment, and the influence of the sample origin on the cytotoxicity of the extracts was evaluated. Extracts obtained from shellfish-derived Penicillia exhibited higher cytotoxicity than the others. Ten of these strains were grown on various media including a medium based on mussel extract (Mytilus edulis), mussel flesh-based medium (MES), to study the influence of the mussel flesh on the production of cytotoxic compounds. The MES host-derived medium was created substituting the yeast extract of YES medium by an aqueous extract of mussel tissues, with other constituent identical to YES medium. When shellfish-derived strains of fungi were grown on MES medium, extracts were found to be more cytotoxic than on the YES medium for some of the strains. HPLC-UV/DAD-MS/MS dereplication of extracts from Penicillium marinum and P. restrictum strains grown on MES medium showed the enhancement of the production of some cytotoxic compounds. The mycotoxin patulin was detected in some P. antarcticum extracts, and its presence seemed to be related to their cytotoxicity. Thus, the enhancement of the toxicity of extracts obtained from shellfish-derived Penicillium strains grown on a host-derived medium, and the production of metabolites such as patulin suggests that a survey of mycotoxins in edible shellfish should be considered. © 2013 The Society for Applied Microbiology.

  1. Concentration of PSP (Paralytic Shellfish Poisoning) Toxin On Shellfish From Inner Ambon Bay and Kao Bay North Halmahera

    NASA Astrophysics Data System (ADS)

    Pello, F. S.; Haumahu, S.; Huliselan, N. V.; Tuapattinaja, M. A.

    2017-10-01

    The Inner Ambon Bay and Kao Bay have potential on fisheries resources which one of them is molluscs. Molluscs especially for class bivalve have economical values and are consumed by coastal community. The research had been done to analyze saxitoxin (STX) concentration on bivalves from Kao Bay and Inner Ambon Bay. The Saxitoxin Elisa Test Kit Protocol was used to determine saxitoxin concentration. The measurement showed that the highest concentration of saxitoxin (392.42 µg STXeq/100g shellfish meat) was Gafrarium tumidum from Ambon Bay, whereas concentration of saxitoxin (321.83 µg STXeq/100g shellfish meat) was Mactra mera from Kao Bay

  2. Food Safety Impacts from Post-Harvest Processing Procedures of Molluscan Shellfish.

    PubMed

    Baker, George L

    2016-04-18

    Post-harvest Processing (PHP) methods are viable food processing methods employed to reduce human pathogens in molluscan shellfish that would normally be consumed raw, such as raw oysters on the half-shell. Efficacy of human pathogen reduction associated with PHP varies with respect to time, temperature, salinity, pressure, and process exposure. Regulatory requirements and PHP molluscan shellfish quality implications are major considerations for PHP usage. Food safety impacts associated with PHP of molluscan shellfish vary in their efficacy and may have synergistic outcomes when combined. Further research for many PHP methods are necessary and emerging PHP methods that result in minimal quality loss and effective human pathogen reduction should be explored.

  3. Impact of heat processing on the detection of the major shellfish allergen tropomyosin in crustaceans and molluscs using specific monoclonal antibodies.

    PubMed

    Kamath, Sandip D; Abdel Rahman, Anas M; Komoda, Toshikazu; Lopata, Andreas L

    2013-12-15

    The major heat-stable shellfish allergen, tropomyosin, demonstrates immunological cross-reactivity, making specific differentiation of crustaceans and molluscs for food labelling very difficult. The aim of this study was to evaluate the application of allergen-specific monoclonal antibodies in differential detection of shellfish-derived tropomyosin in 11 crustacean and 7 mollusc species, and to study the impact of heating on its detection. Cross-reactive tropomyosin was detected in all crustacean species, with partial detection in molluscs: mussels, scallops and snails but none in oyster, octopus and squid. Furthermore, we have demonstrated that heating of shellfish has a profound effect on tropomyosin detection. This was evident by the enhanced recognition of multiple tropomyosin variants in the analysed shellfish species. Specific monoclonal antibodies, targetting the N-terminal region of tropomyosin, must therefore be developed to differentiate tropomyosins in crustaceans and molluscs. This can help in correct food labelling practices and thus protection of consumers. Copyright © 2013 Elsevier Ltd. All rights reserved.

  4. Harmful algal blooms and public health.

    PubMed

    Grattan, Lynn M; Holobaugh, Sailor; Morris, J Glenn

    2016-07-01

    The five most commonly recognized Harmful Algal Bloom-related illnesses are ciguatera poisoning, paralytic shellfish poisoning, neurotoxic shellfish poisoning (NSP), amnesic shellfish poisoning, and diarrhetic shellfish poisoning. Although these exposures result from exposure to different toxins or toxin congeners, these clinical syndromes have much in common. Exposure occurs through the consumption of fish, shellfish, or through exposure to aerosolized NSP toxins. Routine clinical tests are not available for the diagnosis of harmful algal bloom related illnesses, there is no known antidote for exposure, and the risk of these illnesses can negatively impact local fishing and tourism industries. The absence of exposure risk or diagnostic certainty can also precipitate a chain of events that results in considerable psychological distress for coastal populations. Thus, illness prevention is of paramount importance to minimize human and public health risks. To accomplish this, further transdisciplinary research, close communication and collaboration are needed among HAB scientists, public health researchers, and local, state and tribal health departments at academic, community outreach, and policy levels. Copyright © 2016. Published by Elsevier B.V.

  5. A fluorimetric microplate assay for detection and quantitation of toxins causing paralytic shellfish poisoning.

    PubMed

    Louzao, Maria Carmen; Rodriguez Vieytes, Mercedes; Garcia Cabado, Ana; Vieites Baptista De Sousa, Juan Manuel; Botana, Luis Miguel

    2003-04-01

    Paralytic shellfish poisoning is one of the most severe forms of food poisoning. The toxins responsible for this type of poisoning are metabolic products of dinoflagellates, which block neuronal transmission by binding to the voltage-gated Na(+) channel. Accumulation of paralytic toxins in shellfish is an unpredictable phenomenon that necessitates the implementation of a widespread and thorough monitoring program for mollusk toxicity. All of these programs require periodical collection and analysis of a wide range of shellfish. Therefore, development of accurate analytical protocols for the rapid determination of toxicity levels would streamline this process. Our laboratory has developed a fluorimetric microplate bioassay that rapidly and specifically determines the presence of paralytic shellfish toxins in many seafood samples. This method is based on the pharmacological activity of toxins and involves several steps: (i) Incubation of excitable cells in 96 well microtiter plates with the fluorescent dye, bis-oxonol, the distribution of which across the membrane is potential-dependent. (ii) Cell depolarization with veratridine, a sodium channel-activating toxin. (iii) Dose-dependent inhibition of depolarization with saxitoxin or natural samples containing paralytic shellfish toxins. Measuring toxin-induced changes in membrane potential allowed for quantification and estimation of the toxic potency of the samples. This new approach offers significant advantages over classical methods and can be easily automated.

  6. Detection and molecular characterization of norovirus from oysters implicated in outbreaks in the US.

    PubMed

    Woods, Jacquelina W; Calci, Kevin R; Marchant-Tambone, Joey G; Burkhardt, William

    2016-10-01

    Human noroviruses are the leading cause of non-bacterial shellfish associated gastroenteritis. Here we report on the detection and characterization of norovirus (NoV) in shellfish associated outbreaks. Requests were received from state and federal officials for technical assistance in the analysis of shellfish for NoV and male specific coliphage (MSC; an enteric virus surrogate) during the years 2009 thru 2014. In outbreaks where NoV was detected, genogroup II (GII) levels ranged from 2.4 to 82.0 RT-qPCR U/g of digestive diverticula (DD) while NoV genogroup I (GI) levels ranged from 1.5 to 29.8 RT-qPCR U/g of DD. Murine norovirus extraction efficiencies ranged between 50 and 85%. MSC levels ranged from <6 to 80 PFU/100 g. Phylogenetic analysis of the outbreak sequences revealed strains clustering with GI.8, GI.4, GII.3, GII.4, GII.7, and GII.21. There was 100% homology between the shellfish and clinical strains occurring in 2 of 8 outbreaks. Known shellfish consumption data demonstrated probable infectious particles ingested as low as 12. These investigations demonstrate effective detection, quantification, and characterization of NoV in shellfish associated with illness. Published by Elsevier Ltd.

  7. Detection of Tetrodotoxin Shellfish Poisoning (TSP) Toxins and Causative Factors in Bivalve Molluscs from the UK

    PubMed Central

    Turner, Andrew D.; Dhanji-Rapkova, Monika; Coates, Lewis; Bickerstaff, Lesley; Milligan, Steve; O’Neill, Alison; Faulkner, Dermot; McEneny, Hugh; Baker-Austin, Craig; Lees, David N.; Algoet, Myriam

    2017-01-01

    Tetrodotoxins (TTXs) are traditionally associated with the occurrence of tropical Pufferfish Poisoning. In recent years, however, TTXs have been identified in European bivalve mollusc shellfish, resulting in the need to assess prevalence and risk to shellfish consumers. Following the previous identification of TTXs in shellfish from southern England, this study was designed to assess the wider prevalence of TTXs in shellfish from around the coast of the UK. Samples were collected between 2014 and 2016 and subjected to analysis using HILIC-MS/MS. Results showed the continued presence of toxins in shellfish harvested along the coast of southern England, with the maximum concentration of total TTXs reaching 253 µg/kg. TTX accumulation was detected in Pacific oysters (Crassostrea gigas), native oysters (Ostrea edulis) common mussels (Mytilus edulis) and hard clams (Mercenaria mercenaria), but not found in cockles (Cerastoderma edule), razors (Ensis species) or scallops (Pecten maximus). Whilst the highest concentrations were quantified in samples harvested during the warmer summer months, TTXs were still evident during the winter. An assessment of the potential causative factors did not reveal any links with the phytoplankton species Prorocentrum cordatum, instead highlighting a greater level of risk in areas of shallow, estuarine waters with temperatures above 15 °C. PMID:28867772

  8. Forecasting the Human Pathogen Vibrio Parahaemolyticus in Shellfish Tissue within Long Island Sound

    NASA Astrophysics Data System (ADS)

    Whitney, M. M.; DeRosia-Banick, K.

    2016-02-01

    Vibrio parahaemolyticus (Vp) is a marine bacterium that occurs naturally in brackish and saltwater environments and may be found in higher concentrations in the warmest months. Vp is a growing threat to producing safe seafood. Consumption of shellfish with high Vp levels can result in gastrointestinal human illnesses. Management response to Vp-related illness outbreaks includes closure of shellfish growing areas. Water quality observations, Vp measurements, and model forecasts are key components to effective management of shellfish growing areas. There is a clear need for observations within the growing area themselves. These areas are offshore of coastal stations and typically inshore of the observing system moorings. New field observations in Long Island Sound (LIS) shellfish growing areas are described and their agreement with high-resolution satellite sea surface temperature data is discussed. A new dataset of Vp concentrations in shellfish tissue is used to determine the LIS-specific Vp vs. temperature relationship following methods in the FDA pre-harvest Vp risk model. This information is combined with output from a high-resolution hydrodynamic model of LIS to make daily forecasts of Vp levels. The influence of river inflows, the role of heat waves, and predictions for future warmer climates are discussed. The key elements of this observational-modeling approach to pathogen forecasting are extendable to other coastal systems.

  9. Warm temperature acclimation impacts metabolism of paralytic shellfish toxins from Alexandrium minutum in commercial oysters.

    PubMed

    Farrell, Hazel; Seebacher, Frank; O'Connor, Wayne; Zammit, Anthony; Harwood, D Tim; Murray, Shauna

    2015-09-01

    Species of Alexandrium produce potent neurotoxins termed paralytic shellfish toxins and are expanding their ranges worldwide, concurrent with increases in sea surface temperature. The metabolism of molluscs is temperature dependent, and increases in ocean temperature may influence both the abundance and distribution of Alexandrium and the dynamics of toxin uptake and depuration in shellfish. Here, we conducted a large-scale study of the effect of temperature on the uptake and depuration of paralytic shellfish toxins in three commercial oysters (Saccostrea glomerata and diploid and triploid Crassostrea gigas, n = 252 per species/ploidy level). Oysters were acclimated to two constant temperatures, reflecting current and predicted climate scenarios (22 and 27 °C), and fed a diet including the paralytic shellfish toxin-producing species Alexandrium minutum. While the oysters fed on A. minutum in similar quantities, concentrations of the toxin analogue GTX1,4 were significantly lower in warm-acclimated S. glomerata and diploid C. gigas after 12 days. Following exposure to A. minutum, toxicity of triploid C. gigas was not affected by temperature. Generally, detoxification rates were reduced in warm-acclimated oysters. The routine metabolism of the oysters was not affected by the toxins, but a significant effect was found at a cellular level in diploid C. gigas. The increasing incidences of Alexandrium blooms worldwide are a challenge for shellfish food safety regulation. Our findings indicate that rising ocean temperatures may reduce paralytic shellfish toxin accumulation in two of the three oyster types; however, they may persist for longer periods in oyster tissue. © 2015 John Wiley & Sons Ltd.

  10. Prevalence and Molecular Genotyping of Noroviruses in Market Oysters, Mussels, and Cockles in Bangkok, Thailand.

    PubMed

    Kittigul, Leera; Thamjaroen, Anyarat; Chiawchan, Suwat; Chavalitshewinkoon-Petmitr, Porntip; Pombubpa, Kannika; Diraphat, Pornphan

    2016-06-01

    Noroviruses are the most common cause of acute gastroenteritis associated with bivalve shellfish consumption. This study aimed to detect and characterize noroviruses in three bivalve shellfish species: oysters (Saccostrea forskali), cockles (Anadara nodifera), and mussels (Perna viridis). The virus concentration procedure (adsorption-twice elution-extraction) and a molecular method were employed to identify noroviruses in shellfish. RT-nested PCR was able to detect known norovirus GII.4 of 8.8 × 10(-2) genome copies/g of digestive tissues from oyster and cockle concentrates, whereas in mussel concentrates, the positive result was seen at 8.8 × 10(2) copies/g of digestive tissues. From August 2011 to July 2012, a total of 300 shellfish samples, including each of 100 samples from oysters, cockles, and mussels were collected and tested for noroviruses. Norovirus RNA was detected in 12.3 % of shellfish samples. Of the noroviruses, 7.7 % were of the genogroup (G) I, 2.6 % GII, and 2.0 % were mixed GI and GII. The detection rate of norovirus GI was 2.1 times higher than GII. With regards to the different shellfish species, 17 % of the oyster samples were positive, while 14.0 and 6.0 % were positive for noroviruses found in mussels and cockles, respectively. Norovirus contamination in the shellfish occurred throughout the year with the highest peak in September. Seventeen norovirus-positive PCR products were characterized upon a partial sequence analysis of the capsid gene. Based on phylogenetic analysis, five different genotypes of norovirus GI (GI.2, GI.3, GI.4, GI.5, and GI.9) and four different genotypes of GII (GII.1, GII.2, GII.3, and GII.4) were identified. These findings indicate the prevalence and distribution of noroviruses in three shellfish species. The high prevalence of noroviruses in oysters contributes to the optimization of monitoring plans to improve the preventive strategies of acute gastroenteritis.

  11. Evidence of paralytic shellfish poisoning toxin in milkfish in South Taiwan.

    PubMed

    Chou, H-N; Chung, Y-C; Cho, W-C; Chen, C-Y

    2003-06-01

    Natural phytoplankton blooms of the dinoflagellate Alexandrium minutum, milkfish (Chanos chanos) exposed to natural blooms, sediment and mangrove crab (Scylla serrata) were analysed for paralytic shellfish poisoning toxins by high-performance liquid chromatography. The toxin profiles of milkfish and mangrove crab were similar to that of A. minutum collected from blooming fishponds. In a laboratory A. minutum-blooming environment, the stomach and intestine of milkfish accumulated paralytic shellfish poisoning toxins during the exposure period. The non-visceral tissues were non-toxic. However, milkfish lost their entire body burden of toxin on the first day of transferring to a toxic algae-free environment. The result shows that milkfish concentrate paralytic shellfish poisoning toxins in digestive organs and did not retain toxins.

  12. The role of seafood in foodborne diseases in the United States of America.

    PubMed

    Lipp, E K; Rose, J B

    1997-08-01

    In the United States of America, seafood ranked third on the list of products which caused foodborne disease between 1983 and 1992. Outbreaks connected with fish vectors were caused by scombroid, ciguatoxin, bacteria and unknown agents; in shellfish, unknown agents, paralytic shellfish poisoning, Vibrio spp. and other bacteria, followed by hepatitis A virus, were responsible for the outbreaks. At least ten genera of bacterial pathogens have been implicated in seafood-borne diseases. Over the past twenty-five years, bacterial pathogens associated with faecal contamination have represented only 4% of the shellfish-associated outbreaks, while naturally-occurring bacteria accounted for 20% of shellfish-related illnesses and 99% of the deaths. Most of these indigenous bacteria fall into the family Vibrionaceae which includes the genera Vibrio, Aeromonas and Plesiomonas. In general, Vibrio spp. are not associated with faecal contamination and therefore faecal indicators do not correlate with the presence of Vibrio. Viruses are the most significant cause of shellfish-associated disease: in New York State, for example, 33% and 62% of 196 outbreaks between 1981 and 1992 were caused by Norwalk virus and gastrointestinal viruses (small round structured viruses), respectively. In addition, several illnesses are a result of toxic algal blooms, the growth of naturally occurring bacteria and diatoms causing neurotoxic shellfish poisoning, paralytic shellfish poisoning, diarrhoetic shellfish poisoning, amnesic shellfish poisoning and ciguatera. Current estimates place the annual number of ciguatera cases at 20,000 world-wide. Scombroid poisoning is the most significant cause of illness associated with seafood. Scombrotoxin is of bacterial origin and halophilic Vibrio spp. causing high histamine levels are implicated as the source. Scombroid poisoning is geographically diverse and many species have been implicated, namely: tuna, mahi-mahi, bluefish, sardines, mackerel, amberjack and abalone. Temperature abuse has been cited as a major cause of scombroid poisoning. For routine work, the use of faecal indicators to predict the relative level of faecal contamination should not be disposed of. However, the main source of seafood illness is due to species which are not predicted by these organisms. In order to protect public health, routine surveillance using new pathogen-specific techniques such as polymerase chain reaction should be used. This, in combination with risk assessment methods and hazard analysis and critical control points, will begin to address the need for improvement in the safety of seafood.

  13. 46 CFR 221.3 - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ..., catching, taking, or harvesting of fish, shellfish, marine animals, pearls, shells, or marine vegetation or..., or harvesting of fish, shellfish, marine animals, pearls, shells, or marine vegetation. (g) Fish...

  14. 46 CFR 221.3 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ..., catching, taking, or harvesting of fish, shellfish, marine animals, pearls, shells, or marine vegetation or..., or harvesting of fish, shellfish, marine animals, pearls, shells, or marine vegetation. (g) Fish...

  15. 46 CFR 221.3 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ..., catching, taking, or harvesting of fish, shellfish, marine animals, pearls, shells, or marine vegetation or..., or harvesting of fish, shellfish, marine animals, pearls, shells, or marine vegetation. (g) Fish...

  16. 46 CFR 221.3 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ..., catching, taking, or harvesting of fish, shellfish, marine animals, pearls, shells, or marine vegetation or..., or harvesting of fish, shellfish, marine animals, pearls, shells, or marine vegetation. (g) Fish...

  17. 46 CFR 221.3 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ..., catching, taking, or harvesting of fish, shellfish, marine animals, pearls, shells, or marine vegetation or..., or harvesting of fish, shellfish, marine animals, pearls, shells, or marine vegetation. (g) Fish...

  18. Prevalence of fish and shellfish allergy: A systematic review.

    PubMed

    Moonesinghe, Harriet; Mackenzie, Heather; Venter, Carina; Kilburn, Sally; Turner, Paul; Weir, Kellyn; Dean, Taraneh

    2016-09-01

    Accurate information on the prevalence of food allergy facilitates a more evidence-based approach to planning of allergy services and can identify important geographic variations. To conduct a systematic review to assess the age-specific prevalence of fish and shellfish allergy worldwide. Searches were conducted using Web of Science and PubMed. Population-based cross-sectional studies and cohort studies that examined the prevalence of fish and shellfish allergy (IgE mediated and non-IgE mediated) at an identifiable point in time were eligible for inclusion in the study. Reviewers extracted general study information and study design, type of food allergy considered, food(s) assessed, method of diagnosis, sampling strategy, and sample characteristics. Raw data were extracted and percentage prevalence and 95% confidence intervals calculated. A total of 7,333 articles were identified of which 61 studies met the inclusion criteria and were included in this review. The prevalence of fish allergy ranged from 0% to 7% and the prevalence of shellfish allergy from 0% to 10.3%, depending on the method of diagnosis. Where food challenges were used, the prevalence for fish allergy was found to be 0% to 0.3% and for shellfish allergy was 0% to 0.9%. Few studies have established the prevalence of fish or shellfish allergy using the gold standard double-blind, placebo-controlled challenge criteria, with most instead relying on self-reported questionnaire-based methods. The limited data available suggest that fish allergy prevalence is similar worldwide; however, shellfish allergy prevalence may be higher in the Southeast Asia region. Copyright © 2016 American College of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved.

  19. Diarrhetic Shellfish Toxins and Other Lipophilic Toxins of Human Health Concern in Washington State

    PubMed Central

    Trainer, Vera L.; Moore, Leslie; Bill, Brian D.; Adams, Nicolaus G.; Harrington, Neil; Borchert, Jerry; da Silva, Denis A. M.; Eberhart, Bich-Thuy L.

    2013-01-01

    The illness of three people in 2011 after their ingestion of mussels collected from Sequim Bay State Park, Washington State, USA, demonstrated the need to monitor diarrhetic shellfish toxins (DSTs) in Washington State for the protection of human health. Following these cases of diarrhetic shellfish poisoning, monitoring for DSTs in Washington State became formalized in 2012, guided by routine monitoring of Dinophysis species by the SoundToxins program in Puget Sound and the Olympic Region Harmful Algal Bloom (ORHAB) partnership on the outer Washington State coast. Here we show that the DSTs at concentrations above the guidance level of 16 μg okadaic acid (OA) + dinophysistoxins (DTXs)/100 g shellfish tissue were widespread in sentinel mussels throughout Puget Sound in summer 2012 and included harvest closures of California mussel, varnish clam, manila clam and Pacific oyster. Concentrations of toxins in Pacific oyster and manila clam were often at least half those measured in blue mussels at the same site. The primary toxin isomer in shellfish and plankton samples was dinophysistoxin-1 (DTX-1) with D. acuminata as the primary Dinophysis species. Other lipophilic toxins in shellfish were pectenotoxin-2 (PTX-2) and yessotoxin (YTX) with azaspiracid-2 (AZA-2) also measured in phytoplankton samples. Okadaic acid, azaspiracid-1 (AZA-1) and azaspiracid-3 (AZA-3) were all below the levels of detection by liquid chromatography tandem mass spectrometry (LC-MS/MS). A shellfish closure at Ruby Beach, Washington, was the first ever noted on the Washington State Pacific coast due to DSTs. The greater than average Fraser River flow during the summers of 2011 and 2012 may have provided an environment conducive to dinoflagellates and played a role in the prevalence of toxigenic Dinophysis in Puget Sound. PMID:23760013

  20. Diarrhetic shellfish toxins and other lipophilic toxins of human health concern in Washington State.

    PubMed

    Trainer, Vera L; Moore, Leslie; Bill, Brian D; Adams, Nicolaus G; Harrington, Neil; Borchert, Jerry; da Silva, Denis A M; Eberhart, Bich-Thuy L

    2013-05-28

    The illness of three people in 2011 after their ingestion of mussels collected from Sequim Bay State Park, Washington State, USA, demonstrated the need to monitor diarrhetic shellfish toxins (DSTs) in Washington State for the protection of human health. Following these cases of diarrhetic shellfish poisoning, monitoring for DSTs in Washington State became formalized in 2012, guided by routine monitoring of Dinophysis species by the SoundToxins program in Puget Sound and the Olympic Region Harmful Algal Bloom (ORHAB) partnership on the outer Washington State coast. Here we show that the DSTs at concentrations above the guidance level of 16 μg okadaic acid (OA) + dinophysistoxins (DTXs)/100 g shellfish tissue were widespread in sentinel mussels throughout Puget Sound in summer 2012 and included harvest closures of California mussel, varnish clam, manila clam and Pacific oyster. Concentrations of toxins in Pacific oyster and manila clam were often at least half those measured in blue mussels at the same site. The primary toxin isomer in shellfish and plankton samples was dinophysistoxin-1 (DTX-1) with D. acuminata as the primary Dinophysis species. Other lipophilic toxins in shellfish were pectenotoxin-2 (PTX-2) and yessotoxin (YTX) with azaspiracid-2 (AZA-2) also measured in phytoplankton samples. Okadaic acid, azaspiracid-1 (AZA-1) and azaspiracid-3 (AZA-3) were all below the levels of detection by liquid chromatography tandem mass spectrometry (LC-MS/MS). A shellfish closure at Ruby Beach, Washington, was the first ever noted on the Washington State Pacific coast due to DSTs. The greater than average Fraser River flow during the summers of 2011 and 2012 may have provided an environment conducive to dinoflagellates and played a role in the prevalence of toxigenic Dinophysis in Puget Sound.

  1. Cultural Resources Investigations at Redstone Arsenal, Madison County, Alabama. Volume I.

    DTIC Science & Technology

    1980-01-01

    Parmalee and Klippel’s (1974) nutritional I study of freshwater shellfish further confirmed this point of view by demonstrating first that shellfish were...by no means a complete food from the standpoint of human nutrition , and second that they were 1 markedly lower in protein than other locally available...cultural complexity of the Late Archaic shellmound peoples as simply the by-product of the nutritional rich- ness of the shellfish. Instead, the

  2. A role for shellfish aquaculture in coastal nitrogen management.

    PubMed

    Rose, Julie M; Bricker, Suzanne B; Tedesco, Mark A; Wikfors, Gary H

    2014-01-01

    Excess nutrients in the coastal environment have been linked to a host of environmental problems, and nitrogen reduction efforts have been a top priority of resource managers for decades. The use of shellfish for coastal nitrogen remediation has been proposed, but formal incorporation into nitrogen management programs is lagging. Including shellfish aquaculture in existing nitrogen management programs makes sense from environmental, economic, and social perspectives, but challenges must be overcome for large-scale implementation to be possible.

  3. Risk Assessment of Shellfish Toxins

    PubMed Central

    Munday, Rex; Reeve, John

    2013-01-01

    Complex secondary metabolites, some of which are highly toxic to mammals, are produced by many marine organisms. Some of these organisms are important food sources for marine animals and, when ingested, the toxins that they produce may be absorbed and stored in the tissues of the predators, which then become toxic to animals higher up the food chain. This is a particular problem with shellfish, and many cases of poisoning are reported in shellfish consumers each year. At present, there is no practicable means of preventing uptake of the toxins by shellfish or of removing them after harvesting. Assessment of the risk posed by such toxins is therefore required in order to determine levels that are unlikely to cause adverse effects in humans and to permit the establishment of regulatory limits in shellfish for human consumption. In the present review, the basic principles of risk assessment are described, and the progress made toward robust risk assessment of seafood toxins is discussed. While good progress has been made, it is clear that further toxicological studies are required before this goal is fully achieved. PMID:24226039

  4. 76 FR 3695 - Environmental Impact Statement: Interstate 64 Corridor, Virginia

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-20

    ...: Interstate 64 Corridor, Virginia AGENCY: Federal Highway Administration (FHWA), DOT. ACTION: Notice of Intent... Transportation for potential transportation improvements along the Interstate 64 corridor in Virginia. FOR... improvements along the Interstate 64 corridor in Virginia. The approximate limits of the study are Interstate...

  5. Food Allergies

    MedlinePlus

    ... food allergy reactions: milk eggs peanuts soy wheat tree nuts (such as walnuts and cashews) fish shellfish ( ... peanut allergy and about 10% of kids with tree nut allergy outgrow their allergy. Fish and shellfish ...

  6. The impact of depuration on mussel hepatopancreas bacteriome composition and predicted metagenome.

    PubMed

    Rubiolo, J A; Lozano-Leon, A; Rodriguez-Souto, R; Fol Rodríguez, N; Vieytes, M R; Botana, L M

    2018-07-01

    Due to the rapid elimination of bacteria through normal behaviour of filter feeding and excretion, the decontamination of hazardous contaminating bacteria from shellfish is performed by depuration. This process, under conditions that maximize shellfish filtering activity, is a useful method to eliminate microorganisms from bivalves. The microbiota composition in bivalves reflects that of the environment of harvesting waters, so quite different bacteriomes would be expected in shellfish collected in different locations. Bacterial accumulation within molluscan shellfish occurs primarily in the hepatopancreas. In order to assess the effect of the depuration process on these different bacteriomes, in this work we used 16S RNA pyrosequencing and metagenome prediction to assess the impact of 15 h of depuration on the whole hepatopancreas bacteriome of mussels collected in three different locations.

  7. 5 CFR 5001.103 - Impartiality determinations for members of the Interstate Commerce Commission.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... of the Interstate Commerce Commission. 5001.103 Section 5001.103 Administrative Personnel INTERSTATE COMMERCE COMMISSION SUPPLEMENTAL STANDARDS OF ETHICAL CONDUCT FOR EMPLOYEES OF THE INTERSTATE COMMERCE COMMISSION § 5001.103 Impartiality determinations for members of the Interstate Commerce Commission. A member...

  8. 5 CFR 5001.103 - Impartiality determinations for members of the Interstate Commerce Commission.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... of the Interstate Commerce Commission. 5001.103 Section 5001.103 Administrative Personnel INTERSTATE COMMERCE COMMISSION SUPPLEMENTAL STANDARDS OF ETHICAL CONDUCT FOR EMPLOYEES OF THE INTERSTATE COMMERCE COMMISSION § 5001.103 Impartiality determinations for members of the Interstate Commerce Commission. A member...

  9. 5 CFR 5001.103 - Impartiality determinations for members of the Interstate Commerce Commission.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... of the Interstate Commerce Commission. 5001.103 Section 5001.103 Administrative Personnel INTERSTATE COMMERCE COMMISSION SUPPLEMENTAL STANDARDS OF ETHICAL CONDUCT FOR EMPLOYEES OF THE INTERSTATE COMMERCE COMMISSION § 5001.103 Impartiality determinations for members of the Interstate Commerce Commission. A member...

  10. 5 CFR 5001.103 - Impartiality determinations for members of the Interstate Commerce Commission.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... of the Interstate Commerce Commission. 5001.103 Section 5001.103 Administrative Personnel INTERSTATE COMMERCE COMMISSION SUPPLEMENTAL STANDARDS OF ETHICAL CONDUCT FOR EMPLOYEES OF THE INTERSTATE COMMERCE COMMISSION § 5001.103 Impartiality determinations for members of the Interstate Commerce Commission. A member...

  11. 5 CFR 5001.103 - Impartiality determinations for members of the Interstate Commerce Commission.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... of the Interstate Commerce Commission. 5001.103 Section 5001.103 Administrative Personnel INTERSTATE COMMERCE COMMISSION SUPPLEMENTAL STANDARDS OF ETHICAL CONDUCT FOR EMPLOYEES OF THE INTERSTATE COMMERCE COMMISSION § 5001.103 Impartiality determinations for members of the Interstate Commerce Commission. A member...

  12. Choose Fish and Shellfish Wisely

    EPA Pesticide Factsheets

    Information about ways to protect oneself and one's children from eating contaminated fish and shellfish, understanding EPA's advice for safe fish consumption, and learning whether their are fish advisories in one's area.

  13. Shellfish Allergy

    MedlinePlus

    ... of reactions. Learn more here. Milk Egg Peanut Tree Nuts Soy Wheat Fish Shellfish Sesame Other Food Allergens Allergy Alerts Research Programs Research Programs We are the world’s largest private source of food allergy research funding. ...

  14. 7 CFR 301.75-10 - Interstate movement of regulated articles through a quarantined area.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 5 2010-01-01 2010-01-01 false Interstate movement of regulated articles through a... Citrus Canker Notice of Quarantine and Regulations § 301.75-10 Interstate movement of regulated articles... articles being moved interstate, and the date the interstate movement began. (b) The regulated article is...

  15. 27 CFR 7.22a - Voluntary disclosure of major food allergens.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ..., or cod), Crustacean shellfish (for example, crab, lobster, or shrimp), tree nuts (for example... name of the species of Crustacean shellfish (for example, crab, lobster, or shrimp); and (iii) The...

  16. 27 CFR 5.32a - Voluntary disclosure of major food allergens.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ..., or cod), Crustacean shellfish (for example, crab, lobster, or shrimp), tree nuts (for example... of the species of Crustacean shellfish (for example, crab, lobster, or shrimp); and (iii) The names...

  17. 27 CFR 5.32a - Voluntary disclosure of major food allergens.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ..., or cod), Crustacean shellfish (for example, crab, lobster, or shrimp), tree nuts (for example... of the species of Crustacean shellfish (for example, crab, lobster, or shrimp); and (iii) The names...

  18. 27 CFR 7.22a - Voluntary disclosure of major food allergens.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ..., or cod), Crustacean shellfish (for example, crab, lobster, or shrimp), tree nuts (for example... name of the species of Crustacean shellfish (for example, crab, lobster, or shrimp); and (iii) The...

  19. Pregnancy Precautions: FAQs on Pregnancy Hazards

    MedlinePlus

    ... ice cream, and Caesar dressing raw or undercooked fish (sushi), shellfish, or meats paté and meat spreads ... be very well cooked before eating) Also, although fish and shellfish can be an extremely healthy part ...

  20. 40 CFR 125.70 - Purpose and scope.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... propagation of a balanced, indigenous population of shellfish, fish and wildlife in and on the body of water... assure the protection and propagation of a balanced indigenous population of shellfish, fish and wildlife...

  1. 40 CFR 125.70 - Purpose and scope.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... propagation of a balanced, indigenous population of shellfish, fish and wildlife in and on the body of water... assure the protection and propagation of a balanced indigenous population of shellfish, fish and wildlife...

  2. 40 CFR 125.70 - Purpose and scope.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... propagation of a balanced, indigenous population of shellfish, fish and wildlife in and on the body of water... assure the protection and propagation of a balanced indigenous population of shellfish, fish and wildlife...

  3. 40 CFR 125.70 - Purpose and scope.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... propagation of a balanced, indigenous population of shellfish, fish and wildlife in and on the body of water... assure the protection and propagation of a balanced indigenous population of shellfish, fish and wildlife...

  4. 40 CFR 125.70 - Purpose and scope.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... propagation of a balanced, indigenous population of shellfish, fish and wildlife in and on the body of water... assure the protection and propagation of a balanced indigenous population of shellfish, fish and wildlife...

  5. Bio-invasive ascidians in Ireland: A threat for the shellfish industry but also a source of high added value products.

    PubMed

    Palanisamy, Satheesh Kumar; Thomas, Olivier P; P McCormack, Grace

    2018-01-01

    In October 2016, a rapid assessment survey of ascidian species was conducted in shellfish farms at Killary Fjord, in the west of Ireland. Two non-indigenous solitary ascidians Ascidiella aspersa and Corella eumoyta were recorded for the first time in shellfish farms at this location. Both invasive ascidians have the potential to greatly reduce mussel production in Killary Fjord by competing with shellfish for food and habitat. Their high abundance also causes an increase in maintenance costs leading to economic losses for aquaculture farmers. Prompted by our finding of two invasive ascidians in Killary Fjord, we provide a brief review of the ecological role of ascidians and the potential of harnessing biomass from such invasive species for the production of high added value marine natural products.

  6. Effect of the Clean Water Act on shellfish growing waters in the Gulf of Mexico

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Broutman, M.A.

    1988-01-01

    This report examines the classification of shellfish growing waters in the Gulf of Mexico as an indicator of bacterial water quality. Information presented includes the status of classified waters, sources of pollution affecting waters that are not classified as approved, and trends in classification between 1971 and 1985. Data were collected by site visits to the five Gulf states, interviews with state personnel, and reference to written materials. Data are used to assess the effectiveness of national efforts to improve bacterial water quality in the past fifteen years since passage of the Clean Water Act. The hypothesis to be testedmore » is that these efforts have not succeeded in reducing fecal coliform concentrations to levels required for approved harvest of shellfish, as established by the National Shellfish Sanitation Program.« less

  7. Arsenic, cadmium, and manganese levels in shellfish from Map Ta Phut, an industrial area in Thailand, and the potential toxic effects on human cells.

    PubMed

    Rangkadilok, Nuchanart; Siripriwon, Pantaree; Nookabkaew, Sumontha; Suriyo, Tawit; Satayavivad, Jutamaad

    2015-01-01

    Map Ta Phut Industrial Estate is a major industrial area in Thailand for both petrochemical and heavy industries. The release of hazardous wastes and other pollutants from these industries increases the potential for contamination in foods in the surrounding area, especially farmed shellfish. This study determined the arsenic (As), cadmium (Cd), and manganese (Mn) concentrations in the edible flesh of farmed shellfish, including Perna viridis, Meretrix meretrix, and Scapharca inaequivalvis, around the Map Ta Phut area using inductively coupled plasma mass spectrometry. The results showed that shellfish samples contained high levels of total As [1.84-6.42 mg kg(-1) wet weight (ww)]. High Mn concentrations were found in P. viridis and M. meretrix, whereas S. inaequivalis contained the highest Cd. Arsenobetaine (AsB) was found to be the major As species in shellfish (>45% of total As). The in vitro cytotoxicity of these elements was evaluated using human cancer cells (T47D, A549, and Jurkat cells). An observed decrease in cell viability in T47D and Jurkat cells was mainly caused by exposure to inorganic As (iAs) or Mn but not to AsB or Cd. The combined elements (AsB+Mn+Cd) at concentrations predicted to result from the estimated daily intake of shellfish flesh by the local people showed significant cytotoxicity in T47D and Jurkat cells.

  8. Australian shellfish ecosystems: Past distribution, current status and future direction.

    PubMed

    Gillies, Chris L; McLeod, Ian M; Alleway, Heidi K; Cook, Peter; Crawford, Christine; Creighton, Colin; Diggles, Ben; Ford, John; Hamer, Paul; Heller-Wagner, Gideon; Lebrault, Emma; Le Port, Agnès; Russell, Kylie; Sheaves, Marcus; Warnock, Bryn

    2018-01-01

    We review the status of marine shellfish ecosystems formed primarily by bivalves in Australia, including: identifying ecosystem-forming species, assessing their historical and current extent, causes for decline and past and present management. Fourteen species of bivalves were identified as developing complex, three-dimensional reef or bed ecosystems in intertidal and subtidal areas across tropical, subtropical and temperate Australia. A dramatic decline in the extent and condition of Australia's two most common shellfish ecosystems, developed by Saccostrea glomerata and Ostrea angasi oysters, occurred during the mid-1800s to early 1900s in concurrence with extensive harvesting for food and lime production, ecosystem modification, disease outbreaks and a decline in water quality. Out of 118 historical locations containing O. angasi-developed ecosystems, only one location still contains the ecosystem whilst only six locations are known to still contain S. glomerata-developed ecosystems out of 60 historical locations. Ecosystems developed by the introduced oyster Crasostrea gigas are likely to be increasing in extent, whilst data on the remaining 11 ecosystem-forming species are limited, preventing a detailed assessment of their current ecosystem-forming status. Our analysis identifies that current knowledge on extent, physical characteristics, biodiversity and ecosystem services of Australian shellfish ecosystems is extremely limited. Despite the limited information on shellfish ecosystems, a number of restoration projects have recently been initiated across Australia and we propose a number of existing government policies and conservation mechanisms, if enacted, would readily serve to support the future conservation and recovery of Australia's shellfish ecosystems.

  9. Examining Climate Influences and Economic Impacts of Harmful Algal Blooms in Massachusetts: 1993 and 2005

    NASA Astrophysics Data System (ADS)

    Ngo, N.

    2005-12-01

    Although the potential causes of harmful algal blooms (HABs), or red tides, have been studied extensively, the relationships between the environmental drivers and economic impacts have not been fully explored. This paper examined the environmental-economic link by investigating similarities in the environmental conditions leading to the 1993 and 2005 HABs (caused by the dinoflagellate Alexandirum) along the Massachusetts coast, and the resulting effects on shellfish, public health, recreation, tourism, and the commercial shellfish industry in Massachusetts. Environmental influences including sea surface temperature (SST), salinity, precipitation, streamflow, and shellfish toxicity levels were examined for the years 1990 to 2005. Economic impacts on commercial fishery landings (Massachusetts mussel commercial fishery landings and Gloucester commercial fishery landings) were assessed for the years1990 to 2003. The Plume Advection hypothesis was studied and results showed that runoff from the five major rivers that contribute to the Western Maine Coastal Current, the current that carried these cells, peaked in April 1993 and 2005 relative to the mean which varied from river to river. The most intense wind stress coming from the North occurred in April 1993 and May 2005 with speeds of 15-20 m/s. A large decrease in salinity off the Massachusetts coast occurred in May 1993 and measured outside the 68% of 1993 salinity data recorded, and from the information available, in April and May 2005 waters were also less saline. Peaks in shellfish toxicity occurred in early June 1993 at approximately 400 μg toxicity/g shellfish meat and in 2005 at 700 μg toxicity/g shellfish meat. This indicated a lag time between peaks in runoff and toxicity of approximately one month and similarly with decreases in salinity. Runoff also corresponded to a large decrease in salinity during May 1993. Coincidentally, there was also a significant decrease in commercial fishery landings between 1992 and 1993, resulting in a decrease of millions of dollars of revenue for shellfish fishermen and the state of Massachusetts. The 2005 red tide affected 1700 shellfish fisherman and there was a projected loss of 527 million dollars for the 2005 Massachusetts shellfish season. Effects to tourism and public health for 1993 and 2005 were challenging to assess, however, due to scarcity of accurate information. Changing fishery policies affected commercial fishery landings during the same time and were also considered. These results argue for more accurate forecasts that will help predict future HABs, as well as improved methods to provide more reliable information on the economic impacts of HABs to minimize the negative impacts of future HABs.

  10. Occurrence of polybrominated diphenyl ethers in fish and shellfish downstream from electronic-waste recycling plants.

    PubMed

    Jiang, Jinhua; Shi, Shidi; Chen, Tao

    2010-01-01

    We measured 39 polybrominated diphenyl ethers (PBDEs) in the muscle tissue of three species of fish (Sciaenops ocellatus, Sparus macrocephalus, and Lateolabraxjaponicus) and four species of shellfish (Tegillarca granosa, Cyclina sinensis, Sinonovacula constricta, and Ostrea cucullata) that were collected downstream of electronic-waste recycling plants in Taizhou, China. A total of 24 PBDE congeners (PBDE24) in the samples were detected. The sigma PBDE24 (total PBDE) ranged from 545.4 to 1688.7 ng/kg ww (wet weight). The mean sigma PBDE24 concentration was 1382.6 ng/kg ww in fish and 858.1 ng/kg ww in shellfish. The lower brominated congeners were detected at relatively high concentrations in all species. The penta-products, produced from e-waste, were found at relatively low levels. A principal component analysis suggested a significant correlation among di-, tri-, tetra-, and hepta-BDEs for the three species of fish. Similarly, we found a significant correlation between mono- and tri-BDEs in the shellfish. Our results suggested that the processes of PBDE metabolism and elimination were similar in both fish and shellfish. In addition, the primary source of PBDEs appeared to be from the debromination of high brominated PBDEs.

  11. Enteric porcine viruses in farmed shellfish in Denmark.

    PubMed

    Krog, J S; Larsen, L E; Schultz, A C

    2014-09-01

    Bivalve shellfish are at constant risk of being exposed to pathogens as a consequence of contamination of the shellfish beds with human or animal waste originating from sewage treatment plants or slurry fertilized fields. Consumption of contaminated oysters and mussels are frequently reported as causes of disease outbreaks caused by norovirus or hepatitis A virus. Other zoonotic pathogens such as hepatitis E virus (HEV), rotavirus (RV) and Salmonella from livestock may also be transmitted to shellfish via this route. In this study, 29 pooled samples from commercial Danish blue mussels were tested for porcine pathogens and indicator bacteria Escherichia coli (E. coli). All samples tested negative for HEV, RV and Salmonella, whereas E. coli and the highly stable porcine circovirus type 2 (PCV2) were detected in eight and 12 samples, respectively. This is the first study to report the detection of PCV2 in commercial mussels. Based on the detection of PCV2 in clean areas with low prevalence of the normally applied fecal indicator E. coli, testing for PCV2 may be a more sensitive and robust specific porcine waste indicator in shellfish harvesting areas. Copyright © 2014. Published by Elsevier B.V.

  12. Neurotoxic Shellfish Poisoning

    PubMed Central

    Watkins, Sharon M.; Reich, Andrew; Fleming, Lora E.; Hammond, Roberta

    2008-01-01

    Neurotoxic shellfish poisoning (NSP) is caused by consumption of molluscan shellfish contaminated with brevetoxins primarily produced by the dinoflagellate, Karenia brevis. Blooms of K. brevis, called Florida red tide, occur frequently along the Gulf of Mexico. Many shellfish beds in the US (and other nations) are routinely monitored for presence of K. brevis and other brevetoxin-producing organisms. As a result, few NSP cases are reported annually from the US. However, infrequent larger outbreaks do occur. Cases are usually associated with recreationally-harvested shellfish collected during or post red tide blooms. Brevetoxins are neurotoxins which activate voltage-sensitive sodium channels causing sodium influx and nerve membrane depolarization. No fatalities have been reported, but hospitalizations occur. NSP involves a cluster of gastrointestinal and neurological symptoms: nausea and vomiting, paresthesias of the mouth, lips and tongue as well as distal paresthesias, ataxia, slurred speech and dizziness. Neurological symptoms can progress to partial paralysis; respiratory distress has been recorded. Recent research has implicated new species of harmful algal bloom organisms which produce brevetoxins, identified additional marine species which accumulate brevetoxins, and has provided additional information on the toxicity and analysis of brevetoxins. A review of the known epidemiology and recommendations for improved NSP prevention are presented. PMID:19005578

  13. Accumulation of sediment-associated viruses in shellfish.

    PubMed Central

    Landry, E F; Vaughn, J M; Vicale, T J; Mann, R

    1983-01-01

    The present study focused on the importance of contaminated sediments in shellfish accumulation of human viruses. Epifaunal (Crassostrea virginica) and infaunal (Mercenaria mercenaria) shellfish, placed on or in cores, were exposed to either resuspended or undisturbed sediments containing bound poliovirus type 1 (LSc 2ab). Consistent bioaccumulation by oysters (four of five trials) was only noted when sediment-bound viruses occurred in the water column. Virus accumulation was observed in a single instance where sediments remained in an undisturbed state. While the incidence of bioaccumulation was higher with resuspended rather than undisturbed contaminated sediment, the actual concentration of accumulated viruses was not significantly different. The accumulation of viruses from oysters residing on uninoculated sediments. When clams were exposed to undisturbed, virus-contaminated sediments, two of five shellfish pools yielded viral isolates. Bioaccumulation of undisturbed sediments by these bivalves was considered marginal when related to the concentration of virus in contaminated sediments; they would only represent a significant threat when suspended in the water column. Arguments were advanced for water-column sampling in the region of the water-sediment interface to provide an accurate determination of the virological quality of shellfish harvesting waters. PMID:6297392

  14. Dinophysis Toxins: Causative Organisms, Distribution and Fate in Shellfish

    PubMed Central

    Reguera, Beatriz; Riobó, Pilar; Rodríguez, Francisco; Díaz, Patricio A.; Pizarro, Gemita; Paz, Beatriz; Franco, José M.; Blanco, Juan

    2014-01-01

    Several Dinophysis species produce diarrhoetic toxins (okadaic acid and dinophysistoxins) and pectenotoxins, and cause gastointestinal illness, Diarrhetic Shellfish Poisoning (DSP), even at low cell densities (<103 cells·L−1). They are the main threat, in terms of days of harvesting bans, to aquaculture in Northern Japan, Chile, and Europe. Toxicity and toxin profiles are very variable, more between strains than species. The distribution of DSP events mirrors that of shellfish production areas that have implemented toxin regulations, otherwise misinterpreted as bacterial or viral contamination. Field observations and laboratory experiments have shown that most of the toxins produced by Dinophysis are released into the medium, raising questions about the ecological role of extracelular toxins and their potential uptake by shellfish. Shellfish contamination results from a complex balance between food selection, adsorption, species-specific enzymatic transformations, and allometric processes. Highest risk areas are those combining Dinophysis strains with high cell content of okadaates, aquaculture with predominance of mytilids (good accumulators of toxins), and consumers who frequently include mussels in their diet. Regions including pectenotoxins in their regulated phycotoxins will suffer from much longer harvesting bans and from disloyal competition with production areas where these toxins have been deregulated. PMID:24447996

  15. Facts and Statistics about Food Allergies

    MedlinePlus

    ... of reactions. Learn more here. Milk Egg Peanut Tree Nuts Soy Wheat Fish Shellfish Sesame Other Food ... reactions. Eight major food allergens – milk, egg, peanut, tree nuts, wheat, soy, fish and crustacean shellfish – are ...

  16. Red Tide and Paralytic Shellfish Poisoning

    ERIC Educational Resources Information Center

    Dale, Barrie; Yentsch, Clarice M.

    1978-01-01

    Discusses the nature and cause of paralytic shellfish poisoning (PSP). Includes toxic dinoflagellate ecology, taxonomy and life history, and chemistry of the toxins. Recent work with trace metals and directions of future research are also given. (MA)

  17. 78 FR 78783 - Magnuson-Stevens Fishery Conservation and Management Act Provisions; Fisheries of the...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-27

    ... that accumulate in filter-feeding shellfish. Shellfish contaminated with the toxin, if eaten in large... technical correction and will have no effects on fisheries because Massachusetts already exercises its...

  18. 40 CFR 125.57 - Law governing issuance of a section 301(h) modified permit.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... propagation of a balanced indigenous population of shellfish, fish, and wildlife, and allows recreational... application do not support a balanced indigenous population of shellfish, fish, and wildlife, or allow...

  19. 9 CFR 82.10 - Interstate movement of vehicles, cages, coops, containers, troughs, and other equipment from a...

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... after being moved interstate. (c) Before moving interstate any vehicles, cages, coops, containers... other containers or equipment used by or to move pet birds moved interstate in accordance with § 82.5(a... generated by or litter used by the birds or poultry, may be moved interstate from a quarantined area only in...

  20. Shellfish conflict in Baynes Sound: a strategic perspective.

    PubMed

    Hamouda, Luai; Hipel, Keith W; Kilgour, D Marc

    2004-10-01

    The shellfish aquaculture industry (SAI) has operated in Baynes Sound, British Columbia (BC) since the early 1900s. Recognizing the economic potential of the area, the industry has requested additional farming opportunities. However, Baynes Sound upland residents and many other stakeholders have expressed concerns that SAI activities are having a negative impact on the environment, quality of life, and other nonaquaculture resource uses in the area. In order to address these issues, the Action Plan was initiated by a BC government interagency project team in November 2001. To assist in assessing the strategic aspects of this conflict, the decision support system GMCR II is employed here to apply a new methodology, the graph model for conflict resolution, to systematically analyze the ongoing conflict over shellfish aquaculture development in Baynes Sound within a social, economic, and environmental framework. Valuable insights are procured to guide decision-makers toward sustainability of the shellfish industry.

  1. Crab and shellfish occurrences in the newly-grown mangrove habitats in southern Thailand

    NASA Astrophysics Data System (ADS)

    Yeesin, P.; Bautip, S.; Chesoh, S.

    2018-04-01

    Mangrove crabs and shellfish populating in Prince of Songkla University’s new grown mangrove forest were investigated from January 2011 to December 2011 and then repeated annually. A total of 12 species under 6 families of crab and 11 species under 5 families of shellfish were recorded. The most abundant family of crab was Sesarmidae (64.18 %), followed by Ocypodidae, Varunidae, Macrophthalmidae, Portunidae and Dotillidae. Episesarma mederi ( H. Milne Edwards, 1853) showed highest dominant species. In addition, the most dominant family of shellfish was Potamididae (13.79 %), followed by Melampidae, Assimineidae, Onchidiidae and Littorinidae. Sea snail (Cerithidae quadrata; Sowerby, 1866) presented the most dominant coastal mollusc species. Abundance and diversification crabs and mollusks show important component of food web of this type ecosystem. However, only trapped hold samples during low tide were collected but this preliminary finding enables reasonable specified regulation measures.

  2. Mining marine shellfish wastes for bioactive molecules: chitin and chitosan--Part A: extraction methods.

    PubMed

    Hayes, Maria; Carney, Brian; Slater, John; Brück, Wolfram

    2008-07-01

    Legal restrictions, high costs and environmental problems regarding the disposal of marine processing wastes have led to amplified interest in biotechnology research concerning the identification and extraction of additional high grade, low-volume by-products produced from shellfish waste treatments. Shellfish waste consisting of crustacean exoskeletons is currently the main source of biomass for chitin production. Chitin is a polysaccharide composed of N-acetyl-D-glucosamine units and the multidimensional utilization of chitin derivatives including chitosan, a deacetylated derivative of chitin, is due to a number of characteristics including: their polyelectrolyte and cationic nature, the presence of reactive groups, high adsorption capacities, bacteriostatic and fungistatic influences, making them very versatile biomolecules. Part A of this review aims to consolidate useful information concerning the methods used to extract and characterize chitin, chitosan and glucosamine obtained through industrial, microbial and enzymatic hydrolysis of shellfish waste.

  3. Harmful algal blooms and eutrophication: Examining linkages from selected coastal regions of the United States

    PubMed Central

    Anderson, Donald M.; Burkholder, JoAnn M.; Cochlan, William P.; Glibert, Patricia M.; Gobler, Christopher J.; Heil, Cynthia A.; Kudela, Raphael; Parsons, Michael L.; Rensel, J. E. Jack; Townsend, David W.; Trainer, Vera L.; Vargo, Gabriel A.

    2008-01-01

    Coastal waters of the United States (U.S.) are subject to many of the major harmful algal bloom (HAB) poisoning syndromes and impacts. These include paralytic shellfish poisoning (PSP), neurotoxic shellfish poisoning (NSP), amnesic shellfish poisoning (ASP), ciguatera fish poisoning (CFP) and various other HAB phenomena such as fish kills, loss of submerged vegetation, shellfish mortalities, and widespread marine mammal mortalities. Here, the occurrences of selected HABs in a selected set of regions are described in terms of their relationship to eutrophication, illustrating a range of responses. Evidence suggestive of changes in the frequency, extent or magnitude of HABs in these areas is explored in the context of the nutrient sources underlying those blooms, both natural and anthropogenic. In some regions of the U.S., the linkages between HABs and eutrophication are clear and well documented, whereas in others, information is limited, thereby highlighting important areas for further research. PMID:19956363

  4. The changing face of food hypersensitivity in an Asian community.

    PubMed

    Chiang, W C; Kidon, M I; Liew, W K; Goh, A; Tang, J P L; Chay, O M

    2007-07-01

    Food allergy seems to be increasing in Asia as well as world-wide. Our aim was to characterize food protein sensitization patterns in a population of Asian children with possible food allergy. Children presenting to our allergy clinic over 3 years with symptomatic allergic disease and at least one specific food allergen sensitization documented on skin prick testing were included in the analysis. Two hundred and twenty-seven patients fulfilled inclusion criteria. Ninety (40%) of the positive skin tests were positive to egg, 87 (39%) to shellfish, 62 (27.3%) to peanut, 30 (13.2%) to fish, 27 (11.8%) to cow's milk, 21 (9.3%) to sesame, 13 (3.7%) to wheat and eight (3.2%) to soy. Peanut sensitization was the third most common sensitizing allergen, and seen mostly in young atopic children with multiple food hypersensitivities and a family history of atopic dermatitis. The median reported age of first exposure to fish and shellfish was 6 and 12 months, respectively. The mean age at presentation of children with shellfish hypersensitivity was at 6.7 years of age. The likelihood of shellfish sensitization was increased in children with concomitant sensitization to cockroaches. In contrast to previously reported low peanut allergy rates in Asia, in our review, peanut sensitization is present in 27% (62/227) of food-allergic children, mostly in patients with multiple food protein sensitizations. Temporal patterns of first exposure of infants to fish and shellfish are unique to the Asian diet. Shellfish are a major sensitizing food source in Asian children, especially in allergic rhinitis patients sensitized to cockroaches.

  5. Environmental Controls of Shellfish-Toxigenic Vibrio Bacteria in Oregon's Coasts and Estuaries

    NASA Astrophysics Data System (ADS)

    Gradoville, M. R.; Häse, C.; White, A. E.

    2016-02-01

    Vibrio species are a known and persistent concern for economically important aquaculture efforts. In Pacific Northwest shellfish hatcheries, vibriosis, caused by toxigenic Vibrio, has been responsible for major mortality events in recent decades — events that are often irregular and unpredictable. The success of shellfish hatcheries is critically linked to the chemical and biological composition of their seawater resources; thus, it is pertinent to understand the biogeochemical drivers of toxigenic Vibrio in their planktonic state. Recent evidence suggests that vibriosis outbreaks in Netarts Bay, Oregon may be related to wind-driven coastal upwelling, either through the advection of toxigenic Vibrio residing in upwelling source water, or through the creation of favorable growth conditions within the estuary. Here, we present data tracking shellfish-toxigenic Vibrio bacteria in Oregon estuaries and coastal waters through the 2015 upwelling season. Estuarine samples were collected from Netarts Bay and Yaquina Bay in May-October 2015 over a range of upwelling intensities, tidal heights, and biogeochemical conditions. Additionally, samples were collected at multiple depths from offshore coastal Oregon stations in order to test the hypothesis that toxigenic Vibrio reside in upwelling source waters. PCR-based methods were used to quantify the known shellfish pathogen V. coralliilyticus in estuary and seawater samples. These toxigenic Vibrio abundances were correlated to local environmental conditions, including temperature, salinity, carbonate chemistry, nutrients, and chlorophyll a concentrations, as well as coastal wind stress and upwelling intensity. Our analysis aims to define the environmental controls of toxigenic Vibrio in their free-living oceanic state, information that can be used to prevent future disease outbreaks in local shellfish hatcheries.

  6. Australian shellfish ecosystems: Past distribution, current status and future direction

    PubMed Central

    Gillies, Chris L.; McLeod, Ian M.; Alleway, Heidi K.; Cook, Peter; Crawford, Christine; Creighton, Colin; Diggles, Ben; Ford, John; Hamer, Paul; Heller-Wagner, Gideon; Lebrault, Emma; Le Port, Agnès; Russell, Kylie; Sheaves, Marcus; Warnock, Bryn

    2018-01-01

    We review the status of marine shellfish ecosystems formed primarily by bivalves in Australia, including: identifying ecosystem-forming species, assessing their historical and current extent, causes for decline and past and present management. Fourteen species of bivalves were identified as developing complex, three-dimensional reef or bed ecosystems in intertidal and subtidal areas across tropical, subtropical and temperate Australia. A dramatic decline in the extent and condition of Australia’s two most common shellfish ecosystems, developed by Saccostrea glomerata and Ostrea angasi oysters, occurred during the mid-1800s to early 1900s in concurrence with extensive harvesting for food and lime production, ecosystem modification, disease outbreaks and a decline in water quality. Out of 118 historical locations containing O. angasi-developed ecosystems, only one location still contains the ecosystem whilst only six locations are known to still contain S. glomerata-developed ecosystems out of 60 historical locations. Ecosystems developed by the introduced oyster Crasostrea gigas are likely to be increasing in extent, whilst data on the remaining 11 ecosystem-forming species are limited, preventing a detailed assessment of their current ecosystem-forming status. Our analysis identifies that current knowledge on extent, physical characteristics, biodiversity and ecosystem services of Australian shellfish ecosystems is extremely limited. Despite the limited information on shellfish ecosystems, a number of restoration projects have recently been initiated across Australia and we propose a number of existing government policies and conservation mechanisms, if enacted, would readily serve to support the future conservation and recovery of Australia’s shellfish ecosystems. PMID:29444143

  7. Food Allergies: Symptoms, Diagnosis, Prevention and Treatment | NIH MedlinePlus the Magazine

    MedlinePlus

    ... the top eight allergenic foods—eggs, milk, peanuts, tree nuts, soy, wheat, shellfish, and fish. Treating a ... most common trigger foods for anaphylaxis are peanuts, tree nuts, milk, eggs, fish, and crustaceans (shellfish). To ...

  8. 27 CFR 4.32a - Voluntary disclosure of major food allergens.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... shellfish (for example, crab, lobster, or shrimp), tree nuts (for example, almonds, pecans, or walnuts... shellfish (for example, crab, lobster, or shrimp); and (iii) The names “egg” and “peanuts”, as well as the...

  9. 27 CFR 4.32a - Voluntary disclosure of major food allergens.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... shellfish (for example, crab, lobster, or shrimp), tree nuts (for example, almonds, pecans, or walnuts... shellfish (for example, crab, lobster, or shrimp); and (iii) The names “egg” and “peanuts”, as well as the...

  10. 76 FR 65200 - Risk Assessment on Norovirus in Bivalve Molluscan Shellfish: Request for Comments and for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-20

    ..., eaten raw and cooked, including method of cooking (e.g., steaming, frying, or baking); 2. Distribution...., steaming, frying, or baking) for bivalve molluscan shellfish, by species; 4. Experimental data and models...

  11. Mechanisms of viral persistence within bivalves

    USDA-ARS?s Scientific Manuscript database

    Fecal bacteria and waterborne enteric viruses bioconcentrate within bivalve shellfish. However while bacteria are readily purged, viruses tend to be retained within shellfish when bivalves are depurated. The US. Department of Agriculture Seafood Safety Laboratory has recently demonstrated that pha...

  12. Occurrence and Antibiotic Resistance of Vibrio parahaemolyticus from Shellfish in Selangor, Malaysia

    PubMed Central

    Letchumanan, Vengadesh; Pusparajah, Priyia; Tan, Loh Teng-Hern; Yin, Wai-Fong; Lee, Learn-Han; Chan, Kok-Gan

    2015-01-01

    High consumer demand for shellfish has led to the need for large-scale, reliable shellfish supply through aquaculture or shellfish farming. However, bacterial infections which can spread rapidly among shellfish poses a major threat to this industry. Shellfish farmers therefore often resort to extensive use of antibiotics, both prophylactically and therapeutically, in order to protect their stocks. The extensive use of antibiotics in aquaculture has been postulated to represent a major contributing factor in the rising incidence of antimicrobial resistant pathogenic bacteria in shellfish. This study aimed to investigate the incidence of pathogenic Vibrio parahaemolyticus and determine the antibiotic resistance profile as well as to perform plasmid curing in order to determine the antibiotic resistance mediation. Based on colony morphology, all 450 samples tested were positive for Vibrio sp; however, tox-R assay showed that only 44.4% (200/450) of these were V. parahaemolyticus. Out of these 200 samples, 6.5% (13/200) were trh-positive while none were tdh-positive. Antibiotic resistance was determined for all V. parahaemolyticus identified against 14 commonly used antibiotics and the multiple antibiotic resistance index (MAR) was calculated. The isolates demonstrated high resistance to several antibiotics tested- including second and third-line antibiotics- with 88% resistant to ampicillin, 81% to amikacin,70.5% to kanamycin, 73% to cefotaxime, and 51.5% to ceftazidime. The MAR index ranged from 0.00 to 0.79 with the majority of samples having an index of 0.36 (resistant to five antibiotics). Among the 13 trh-positive strains, almost 70% (9/13) demonstrated resistance to 4 or more antibiotics. Plasmid profiling for all V. parahaemolyticus isolates revealed that 86.5% (173/200) contained plasmids - ranging from 1 to 7 plasmids with DNA band sizes ranging from 1.2 kb to greater than 10 kb. 6/13 of the pathogenic V. pathogenic strains contained plasmid. After plasmid curing, the plasmid containing pathogenic strains isolated in our study have chromosomally mediated ampicillin resistance while the remaining resistance phenotypes are plasmid mediated. Overall, our results indicate that while the incidence of pathogenic V. parahaemolyticus in shellfish in Selangor still appears to be at relatively reassuring levels, antibiotic resistance is a real concern and warrants ongoing surveillance. PMID:26697003

  13. PCDD and PCDF exposures among fishing community through intake of fish and shellfish from the Straits of Malacca.

    PubMed

    Azlan, Azrina; Nasir, Nurul Nadiah Mohamad; Shamsudin, Norashikin; Rahman, Hejar Abdul; Khoo, Hock Eng; Razman, Muhammad Rizal

    2015-07-21

    Exposure to PCDD/PCDF (dioxin and furan) through consumption of fish and shellfish is closely related to the occurrence of skin diseases, such as chloracne and hyperpigmentation. This study aimed to determine the exposure of PCDD/PCDF and its congeners in fish and shellfish obtained from different regions of the Straits of Malacca among the fishing community. The risk of fish and shellfish consumption and exposure to PCDD/PCDF among fishermen living in coastal areas of the Straits were evaluated based on a cross-sectional study involving face to face interviews, blood pressure and anthropometric measurements, and administration of food frequency questionnaires (FFQ). Skin examination was done by a dermatologist after the interview session. Determination of 17 congeners of PCDD/PCDF in 48 composite samples of fish and shellfish was performed based on HRGC/HRMS analysis. The total PCDD/PCDF in the seafood samples ranged from 0.12 to 1.24 pg WHO-TEQ/g fresh weight (4.6-21.8 pg WHO-TEQ/g fat). No significant difference found for the concentrations of PCDD/PCDF between the same types of seafood samples obtained from the three different regions. The concentrations of the most potent congener, 2,3,7,8-TCDD in the seafood samples ranged from 0.01 to 0.11 pg WHO-TEQ/g FW (1.9 pg WHO-TEQ/g fat). A positive moderate correlation was found between the fat contents and concentrations of PCDD/PCDF determined in the seafood samples. The total PCDD/PCDF in all seafood samples were below the 1 pg WHO-TEQ/g fresh weight, with the exception of grey eel-catfish. The respondents had consumed fish and shellfish with the amounts ranging between 2.02 g and 44.06 g per person per day. The total PCDD/PCDF exposures through consumption of fish and shellfish among the respondents were between 0.01 and 0.16 pg WHO-TEQ/kg BW/day. With regard to the two PCDD/PCDF-related skin diseases, no chloracne case was found among the respondents, but 2.2% of the respondents were diagnosed to have hyperpigmentation. Intake of a moderate amount of fish and shellfish from the area is safe and does not pose a risk for skin diseases. An over-consumption of seafood from the potentially polluted area of the Straits should be monitored in future.

  14. 77 FR 77004 - Data Standards for Electronic Interstate Certificates of Veterinary Inspection

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-31

    ...] Data Standards for Electronic Interstate Certificates of Veterinary Inspection AGENCY: Animal and Plant... data standards required to generate an official interstate certificate of veterinary inspection (ICVI... interstate certificate of veterinary inspection (ICVI). The standards were developed with the National...

  15. Novel methods for detection of foodborne viruses

    USDA-ARS?s Scientific Manuscript database

    Enteric viruses such as norovirus are the number one cause of foodborne illness. Bivalve shellfish such as oysters efficiently bioconcentrate and retain theses pathogens, making raw shellfish consumption a significant risk factor for acquisition of these viruses. Recent ARS research indicates...

  16. Health risk/benefit information for consumers of fish and shellfish: FishChoice, a new online tool.

    PubMed

    Vilavert, Lolita; Borrell, Ferran; Nadal, Martí; Jacobs, Silke; Minnens, Fien; Verbeke, Wim; Marques, António; Domingo, José Luis

    2017-06-01

    It is well known that due to the content in omega-3 fatty acids, consumption of fish and shellfish is beneficial for human cardiovascular health. However, a number of recent studies have shown that fish consumption may be also a potential dietary source of exposure to various environmental pollutants with well-known potential adverse effects on human health. Moreover, there is still a lack of information regarding levels of emerging contaminants in fish and shellfish, in particular among consumers and stakeholders. Within the ECsafeSEAFOOD FP7 project, a wide variety of emerging contaminants including brominated flame retardants, pharmaceuticals, perfluoroalkyl substances, musk fragrances, polycyclic aromatic hydrocarbons, UV-filters and endocrine disruptors, as well as inorganic arsenic and methylmercury, were analyzed in fish and shellfish samples collected all over the European Union. These data, together with those regarding nutrient concentrations from different European food composition databases, were integrated into a new online tool, called FishChoice. In this paper, we report how FishChoice was designed and present its main improvements compared to previous tools or software programs, in terms of selected pollutants, number of species, and specific recommendations for an optimal consumption of fish and shellfish. Copyright © 2017 Elsevier Ltd. All rights reserved.

  17. Algae-Based Carbon Sequestration

    NASA Astrophysics Data System (ADS)

    Haoyang, Cai

    2018-03-01

    Our civilization is facing a series of environmental problems, including global warming and climate change, which are caused by the accumulation of green house gases in the atmosphere. This article will briefly analyze the current global warming problem and propose a method that we apply algae cultivation to absorb carbon and use shellfish to sequestrate it. Despite the importance of decreasing CO2 emissions or developing carbon-free energy sources, carbon sequestration should be a key issue, since the amount of carbon dioxide that already exists in the atmosphere is great enough to cause global warming. Algae cultivation would be a good choice because they have high metabolism rates and provides shellfish with abundant food that contains carbon. Shellfish’s shells, which are difficult to be decomposed, are reliable storage of carbon, compared to dead organisms like trees and algae. The amount of carbon that can be sequestrated by shellfish is considerable. However, the sequestrating rate of algae and shellfish is not high enough to affect the global climate. Research on algae and shellfish cultivation, including gene technology that aims to create “super plants” and “super shellfish”, is decisive to the solution. Perhaps the baton of history will shift to gene technology, from nuclear physics that has lost appropriate international environment after the end of the Cold War. Gene technology is vital to human survival.

  18. Recombinase polymerase amplification combined with lateral flow dipstick for equipment-free detection of Salmonella in shellfish.

    PubMed

    Gao, Weifang; Huang, Hailong; Zhu, Peng; Yan, Xiaojun; Fan, Jianzhong; Jiang, Jinpo; Xu, Jilin

    2018-05-01

    Salmonella is a major pathogen that causes acute foodborne outbreaks worldwide. Seafood, particularly shellfish, is a proven source of Salmonella spp. infection because many people prefer to eat it raw or lightly cooked. However, traditional identification methods are too time-consuming and complex to detect contamination of bacteria in the food chain in a timely manner, and few studies have aimed to identify Salmonella in shellfish early in the supply chain. We herein developed a method for rapid detection of Salmonella in shellfish based on the method of recombinase polymerase amplification (RPA) combined with lateral flow dipstick (LFD), which targets the invasion gene A (invA). The RPA-LFD was able to function at 30-45 °C, and at the temperature of 40 °C, it only took 8 min of amplification to reach the test threshold of amplicons. The established method had both a good specificity and a sensitivity of 100 fg DNA per reaction (20 µL). Regarding practical performance, RPA-LFD performed better than real-time PCR. Another advantage of RPA-LFD is that it was capable of being performed without expensive equipments. Thus, RPA-LFD has potential for further development as a detection kit for Salmonella in shellfish and other foods under field conditions.

  19. Single-laboratory validation of the microplate receptor binding assay for paralytic shellfish toxins in shellfish.

    PubMed

    Van Dolah, Frances M; Leighfield, Tod A; Doucette, Gregory J; Bean, Laurie; Niedzwiadek, Barbara; Rawn, Dorothea F K

    2009-01-01

    A single-laboratory validation (SLV) study was conducted for the microplate receptor binding assay (RBA) for paralytic shellfish poisoning (PSP) toxins in shellfish. The basis of the assay is the competition between [3H]saxitoxin (STX) and STX in a standard or sample for binding to the voltage dependent sodium channel. A calibration curve is generated by the addition of 0.01-1000 nM STX, which results in the concentration dependent decrease in [3H]STX-receptor complexes formed and serves to quantify STX in unknown samples. This study established the LOQ, linearity, recovery, accuracy, and precision of the assay for determining PSP toxicity in shellfish extracts, as performed by a single analyst on multiple days. The standard curve obtained on 5 independent days resulted in a half-maximal inhibition (IC50) of 2.3 nM STX +/- 0.3 (RSD = 10.8%) with a slope of 0.96 +/- 0.06 (RSD = 6.3%) and a dynamic range of 1.2-10.0 nM. The LOQ was 5.3 microg STX equivalents/100 g shellfish. Linearity, established by quantification of three levels of purified STX (1.5, 3, and 6 nM), yielded an r2 of 0.97. Recovery from mussels spiked with three levels (40, 80, and 120 microg STX/100 g) averaged 121%. Repeatability (RSD(r)), determined on six naturally contaminated shellfish samples on 5 independent days, was 17.7%. A method comparison with the AOAC mouse bioassay yielded r2 = 0.98 (slope = 1.29) in the SLV study. The effects of the extraction method on RBA-based toxicity values were assessed on shellfish extracted for PSP toxins using the AOAC mouse bioassay method (0.1 M HCI) compared to that for the precolumn oxidation HPLC method (0.1% acetic acid). The two extraction methods showed linear correlation (r2 = 0.99), with the HCl extraction method yielding slightly higher toxicity values (slope = 1.23). A similar relationship was observed between HPLC quantification of the HCI- and acetic acid-extracted samples (r2 = 0.98, slope 1.19). The RBA also had excellent linear correlation with HPLC analyses (r2 = 0.98 for HCl, r2 = 0.99 for acetic acid), but gave somewhat higher values than HPLC using either extraction method (slope = 1.39 for HCl extracts, slope = 1.32 for acetic acid). Overall, the excellent linear correlations with the both mouse bioassay and HPLC method and sufficient interassay repeatability suggest that the RBA can be effective as a high throughput screen for estimating PSP toxicity in shellfish.

  20. 40 CFR 52.352 - Interstate transport.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 3 2011-07-01 2011-07-01 false Interstate transport. 52.352 Section 52...) APPROVAL AND PROMULGATION OF IMPLEMENTATION PLANS Colorado § 52.352 Interstate transport. Addition to the Colorado State Implementation Plan of the Colorado Interstate Transport SIP regarding the 1997 8-Hour Ozone...

  1. 40 CFR 52.352 - Interstate transport.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 3 2010-07-01 2010-07-01 false Interstate transport. 52.352 Section 52...) APPROVAL AND PROMULGATION OF IMPLEMENTATION PLANS Colorado § 52.352 Interstate transport. Addition to the Colorado State Implementation Plan of the Colorado Interstate Transport SIP regarding the 1997 8-Hour Ozone...

  2. 40 CFR 52.283 - Interstate Transport.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 3 2011-07-01 2011-07-01 false Interstate Transport. 52.283 Section 52...) APPROVAL AND PROMULGATION OF IMPLEMENTATION PLANS California § 52.283 Interstate Transport. Link to an... Resources Board submitted the “Interstate Transport State Implementation Plan (SIP) for the 1997 8-hour...

  3. 40 CFR 52.2354 - Interstate transport.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 4 2011-07-01 2011-07-01 false Interstate transport. 52.2354 Section...) APPROVAL AND PROMULGATION OF IMPLEMENTATION PLANS (CONTINUED) Utah § 52.2354 Interstate transport. CAA..., Interstate Transport, of the Utah SIP submitted by the Utah Governor on March 22, 2007, satisfies the...

  4. 40 CFR 52.2354 - Interstate transport.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 4 2010-07-01 2010-07-01 false Interstate transport. 52.2354 Section...) APPROVAL AND PROMULGATION OF IMPLEMENTATION PLANS (CONTINUED) Utah § 52.2354 Interstate transport. CAA..., Interstate Transport, of the Utah SIP submitted by the Utah Governor on March 22, 2007, satisfies the...

  5. 40 CFR 81.51 - Portland Interstate Air Quality Control Region.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 18 2013-07-01 2013-07-01 false Portland Interstate Air Quality...) AIR PROGRAMS (CONTINUED) DESIGNATION OF AREAS FOR AIR QUALITY PLANNING PURPOSES Designation of Air Quality Control Regions § 81.51 Portland Interstate Air Quality Control Region. The Portland Interstate...

  6. 49 CFR 374.201 - Prohibition against smoking on interstate passenger-carrying motor vehicles.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 49 Transportation 5 2012-10-01 2012-10-01 false Prohibition against smoking on interstate... MOTOR CARRIER SAFETY REGULATIONS PASSENGER CARRIER REGULATIONS Limitation of Smoking on Interstate Passenger Carrier Vehicles § 374.201 Prohibition against smoking on interstate passenger-carrying motor...

  7. 49 CFR 374.201 - Prohibition against smoking on interstate passenger-carrying motor vehicles.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 49 Transportation 5 2011-10-01 2011-10-01 false Prohibition against smoking on interstate... MOTOR CARRIER SAFETY REGULATIONS PASSENGER CARRIER REGULATIONS Limitation of Smoking on Interstate Passenger Carrier Vehicles § 374.201 Prohibition against smoking on interstate passenger-carrying motor...

  8. 49 CFR 374.201 - Prohibition against smoking on interstate passenger-carrying motor vehicles.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 49 Transportation 5 2014-10-01 2014-10-01 false Prohibition against smoking on interstate... MOTOR CARRIER SAFETY REGULATIONS PASSENGER CARRIER REGULATIONS Limitation of Smoking on Interstate Passenger Carrier Vehicles § 374.201 Prohibition against smoking on interstate passenger-carrying motor...

  9. 49 CFR 374.201 - Prohibition against smoking on interstate passenger-carrying motor vehicles.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 5 2010-10-01 2010-10-01 false Prohibition against smoking on interstate... MOTOR CARRIER SAFETY REGULATIONS PASSENGER CARRIER REGULATIONS Limitation of Smoking on Interstate Passenger Carrier Vehicles § 374.201 Prohibition against smoking on interstate passenger-carrying motor...

  10. 49 CFR 374.201 - Prohibition against smoking on interstate passenger-carrying motor vehicles.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 49 Transportation 5 2013-10-01 2013-10-01 false Prohibition against smoking on interstate... MOTOR CARRIER SAFETY REGULATIONS PASSENGER CARRIER REGULATIONS Limitation of Smoking on Interstate Passenger Carrier Vehicles § 374.201 Prohibition against smoking on interstate passenger-carrying motor...

  11. 40 CFR 81.51 - Portland Interstate Air Quality Control Region.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 17 2010-07-01 2010-07-01 false Portland Interstate Air Quality...) AIR PROGRAMS (CONTINUED) DESIGNATION OF AREAS FOR AIR QUALITY PLANNING PURPOSES Designation of Air Quality Control Regions § 81.51 Portland Interstate Air Quality Control Region. The Portland Interstate...

  12. 21 CFR 161.130 - Oysters.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... water. Before packing into the containers for shipment or other delivery for consumption the oysters are... FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) FOOD FOR HUMAN CONSUMPTION FISH AND SHELLFISH Requirements for Specific Standardized Fish and Shellfish § 161.130 Oysters. (a...

  13. Rapid extraction of virus-contaminated hemocytes from oysters

    USDA-ARS?s Scientific Manuscript database

    Rapid viral detection methods are necessary to employ diagnostic testing for viral contamination in shellfish to prevent and control foodborne illness. Current shellfish viral RNA extraction methods, which are time-consuming and not applicable for routine monitoring, require the testing of whole or ...

  14. Hemocytes are sites of persistence for virus-contaminated oysters

    USDA-ARS?s Scientific Manuscript database

    Like fecal bacteria, waterborne enteric viruses are readily bioconcentrated by bivalve shellfish. However while many bacteria decline rapidly when bivalves are placed in uncontaminated water, viruses tend to be retained within shellfish. In this study, we offer evidence that phagocytic blood cells...

  15. Shellfish-associated enteric virus illness: virus localization, disease outbreaks and prevention

    USDA-ARS?s Scientific Manuscript database

    Numerous outbreaks of shellfish-borne enteric virus illness have been reported worldwide. Most notable among the outbreaks are those involving norovirus illness and hepatitis A. Lessons learned from outbreak investigations indicate that most outbreaks are preventable. Anthropogenic sources of con...

  16. 17 CFR 250.8 - Exemption of subsidiaries subject to jurisdiction of Interstate Commerce Commission.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... subject to jurisdiction of Interstate Commerce Commission. 250.8 Section 250.8 Commodity and Securities... to jurisdiction of Interstate Commerce Commission. Any subsidiary company of a registered holding company, which subsidiary is subject to the jurisdiction of the Interstate Commerce Commission but is not...

  17. 17 CFR 250.8 - Exemption of subsidiaries subject to jurisdiction of Interstate Commerce Commission.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... subject to jurisdiction of Interstate Commerce Commission. 250.8 Section 250.8 Commodity and Securities... to jurisdiction of Interstate Commerce Commission. Any subsidiary company of a registered holding company, which subsidiary is subject to the jurisdiction of the Interstate Commerce Commission but is not...

  18. 7 CFR 301.74 - Restrictions on interstate movement of regulated articles.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 5 2010-01-01 2010-01-01 false Restrictions on interstate movement of regulated articles. 301.74 Section 301.74 Agriculture Regulations of the Department of Agriculture (Continued) ANIMAL... § 301.74 Restrictions on interstate movement of regulated articles. No person may move interstate from...

  19. 9 CFR 85.9 - Other interstate movements.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Other interstate movements. 85.9... Other interstate movements. The Administrator may, upon request in specific cases, permit the interstate movement of livestock not otherwise provided for in this part under such conditions as he may prescribe to...

  20. 7 CFR 301.86 - Restrictions on interstate movement of regulated articles.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 5 2010-01-01 2010-01-01 false Restrictions on interstate movement of regulated articles. 301.86 Section 301.86 Agriculture Regulations of the Department of Agriculture (Continued) ANIMAL... Nematode § 301.86 Restrictions on interstate movement of regulated articles. No person may move interstate...

  1. 40 CFR 81.85 - Metropolitan Sioux Falls Interstate Air Quality Control Region.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 18 2014-07-01 2014-07-01 false Metropolitan Sioux Falls Interstate... Designation of Air Quality Control Regions § 81.85 Metropolitan Sioux Falls Interstate Air Quality Control Region. The Metropolitan Sioux Falls Interstate Air Quality Control Region (Iowa-South Dakota) has been...

  2. 40 CFR 81.85 - Metropolitan Sioux Falls Interstate Air Quality Control Region.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 17 2010-07-01 2010-07-01 false Metropolitan Sioux Falls Interstate... Designation of Air Quality Control Regions § 81.85 Metropolitan Sioux Falls Interstate Air Quality Control Region. The Metropolitan Sioux Falls Interstate Air Quality Control Region (Iowa-South Dakota) has been...

  3. 40 CFR 81.85 - Metropolitan Sioux Falls Interstate Air Quality Control Region.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 18 2012-07-01 2012-07-01 false Metropolitan Sioux Falls Interstate... Designation of Air Quality Control Regions § 81.85 Metropolitan Sioux Falls Interstate Air Quality Control Region. The Metropolitan Sioux Falls Interstate Air Quality Control Region (Iowa-South Dakota) has been...

  4. 40 CFR 81.85 - Metropolitan Sioux Falls Interstate Air Quality Control Region.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 17 2011-07-01 2011-07-01 false Metropolitan Sioux Falls Interstate... Designation of Air Quality Control Regions § 81.85 Metropolitan Sioux Falls Interstate Air Quality Control Region. The Metropolitan Sioux Falls Interstate Air Quality Control Region (Iowa-South Dakota) has been...

  5. 40 CFR 81.85 - Metropolitan Sioux Falls Interstate Air Quality Control Region.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 18 2013-07-01 2013-07-01 false Metropolitan Sioux Falls Interstate... Designation of Air Quality Control Regions § 81.85 Metropolitan Sioux Falls Interstate Air Quality Control Region. The Metropolitan Sioux Falls Interstate Air Quality Control Region (Iowa-South Dakota) has been...

  6. 40 CFR 81.94 - Shreveport-Texarkana-Tyler Interstate Air Quality Control Region.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 17 2011-07-01 2011-07-01 false Shreveport-Texarkana-Tyler Interstate... Designation of Air Quality Control Regions § 81.94 Shreveport-Texarkana-Tyler Interstate Air Quality Control Region. The Shreveport-Texarkana-Tyler Interstate Air Quality Control Region (Arkansas-Louisiana-Oklahoma...

  7. 40 CFR 81.94 - Shreveport-Texarkana-Tyler Interstate Air Quality Control Region.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 17 2010-07-01 2010-07-01 false Shreveport-Texarkana-Tyler Interstate... Designation of Air Quality Control Regions § 81.94 Shreveport-Texarkana-Tyler Interstate Air Quality Control Region. The Shreveport-Texarkana-Tyler Interstate Air Quality Control Region (Arkansas-Louisiana-Oklahoma...

  8. 40 CFR 81.94 - Shreveport-Texarkana-Tyler Interstate Air Quality Control Region.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 18 2014-07-01 2014-07-01 false Shreveport-Texarkana-Tyler Interstate... Designation of Air Quality Control Regions § 81.94 Shreveport-Texarkana-Tyler Interstate Air Quality Control Region. The Shreveport-Texarkana-Tyler Interstate Air Quality Control Region (Arkansas-Louisiana-Oklahoma...

  9. 40 CFR 81.94 - Shreveport-Texarkana-Tyler Interstate Air Quality Control Region.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 18 2012-07-01 2012-07-01 false Shreveport-Texarkana-Tyler Interstate... Designation of Air Quality Control Regions § 81.94 Shreveport-Texarkana-Tyler Interstate Air Quality Control Region. The Shreveport-Texarkana-Tyler Interstate Air Quality Control Region (Arkansas-Louisiana-Oklahoma...

  10. 40 CFR 81.94 - Shreveport-Texarkana-Tyler Interstate Air Quality Control Region.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 18 2013-07-01 2013-07-01 false Shreveport-Texarkana-Tyler Interstate... Designation of Air Quality Control Regions § 81.94 Shreveport-Texarkana-Tyler Interstate Air Quality Control Region. The Shreveport-Texarkana-Tyler Interstate Air Quality Control Region (Arkansas-Louisiana-Oklahoma...

  11. 40 CFR 81.102 - Metropolitan Quad Cities Interstate Air Quality Control Region.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 17 2011-07-01 2011-07-01 false Metropolitan Quad Cities Interstate... Designation of Air Quality Control Regions § 81.102 Metropolitan Quad Cities Interstate Air Quality Control Region. The Metropolitan Quad Cities Interstate Air Quality Control Region (Illinois-Iowa) consists of...

  12. 40 CFR 81.102 - Metropolitan Quad Cities Interstate Air Quality Control Region.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 17 2010-07-01 2010-07-01 false Metropolitan Quad Cities Interstate... Designation of Air Quality Control Regions § 81.102 Metropolitan Quad Cities Interstate Air Quality Control Region. The Metropolitan Quad Cities Interstate Air Quality Control Region (Illinois-Iowa) consists of...

  13. 40 CFR 81.25 - Metropolitan Kansas City Interstate Air Quality Control Region.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 17 2011-07-01 2011-07-01 false Metropolitan Kansas City Interstate... Designation of Air Quality Control Regions § 81.25 Metropolitan Kansas City Interstate Air Quality Control Region. The Metropolitan Kansas City Interstate Air Quality Control Region (Missouri-Kansas) consists of...

  14. 40 CFR 81.25 - Metropolitan Kansas City Interstate Air Quality Control Region.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 17 2010-07-01 2010-07-01 false Metropolitan Kansas City Interstate... Designation of Air Quality Control Regions § 81.25 Metropolitan Kansas City Interstate Air Quality Control Region. The Metropolitan Kansas City Interstate Air Quality Control Region (Missouri-Kansas) consists of...

  15. 75 FR 52935 - Colorado Interstate Gas Company; Notice of Application

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-30

    ... Interstate Gas Company (CIG), P.O. Box 1087, Colorado Springs, Colorado 80944, pursuant to section 7(c) of.... Susan C. Stires, Director, Regulatory Affairs, Colorado Interstate Gas Company, P.O. Box 1087, Colorado... President and General Counsel, Colorado Interstate Gas Company; P.O. Box 1087, Colorado Springs, Colorado...

  16. 76 FR 29290 - Environmental Impact Statement: Interstate 64 Hampton Roads Bridge Tunnel Corridor, Virginia

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-20

    ...: Interstate 64 Hampton Roads Bridge Tunnel Corridor, Virginia AGENCY: Federal Highway Administration (FHWA... Interstate 64 Hampton Roads Bridge Tunnel (HRBT) corridor in Virginia. FOR FURTHER INFORMATION CONTACT... Interstate 64 Hampton Roads Bridge Tunnel (HRBT) corridor in Virginia. The approximate limits of the study...

  17. The ability of algal organic matter and surface runoff to promote the abundance of pathogenic and non-pathogenic strains of Vibrio parahaemolyticus in Long Island Sound, USA

    PubMed Central

    Thickman, Jake D.

    2017-01-01

    Food safety is a major concern in the shellfish industry, as severe illness can result from consuming shellfish that have accumulated waterborne pathogens. Shellfish harvesting areas are typically monitored for indicator bacteria such as fecal coliforms that serve as proxies for enteric pathogens although these indicators have shown little relation to some naturally occurring pathogenic bacteria such as Vibrio parahaemolyticus. To examine the dynamics and ecology of pathogenic and non-pathogenic strains of V. parahaemolyticus and address the relevance of indicator bacteria in predicting V. parahaemolyticus concentrations, field surveys and experiments were carried out in western Long Island Sound, NY, USA, a region that has experienced recent outbreaks of shellfish contaminated with V. parahaemolyticus. Pathogenic and non-pathogenic strains were quantified via PCR detection of marker genes and most probable number techniques. Field survey data showed little correspondence between fecal coliforms and V. parahaemolyticus, but significant correlations between V. parahaemolyticus and an alternative indicator, enterococci, and between V. parahaemolyticus and short-term (48 h) rainfall were observed. Experiments demonstrated that enrichment of seawater with phytoplankton-derived dissolved organic matter significantly increased the concentration of total V. parahaemolyticus and the presence pathogenic V. parahaemolyticus, but higher temperatures did not. Collectively, these study results suggest that fecal coliforms may fail to account for the full suite of important shellfish pathogens but that enterococci could provide a potential alternative or supplement to shellfish sanitation monitoring. Given the ability of algal-derived dissolved organic matter to promote the growth of pathogenic V. parahaemolyticus, restricting nutrient inputs into coastal water bodies that promote algal blooms may indirectly decrease the proliferation of V. parahaemolyticus and protect public health. PMID:29020074

  18. The ability of algal organic matter and surface runoff to promote the abundance of pathogenic and non-pathogenic strains of Vibrio parahaemolyticus in Long Island Sound, USA.

    PubMed

    Thickman, Jake D; Gobler, Christopher J

    2017-01-01

    Food safety is a major concern in the shellfish industry, as severe illness can result from consuming shellfish that have accumulated waterborne pathogens. Shellfish harvesting areas are typically monitored for indicator bacteria such as fecal coliforms that serve as proxies for enteric pathogens although these indicators have shown little relation to some naturally occurring pathogenic bacteria such as Vibrio parahaemolyticus. To examine the dynamics and ecology of pathogenic and non-pathogenic strains of V. parahaemolyticus and address the relevance of indicator bacteria in predicting V. parahaemolyticus concentrations, field surveys and experiments were carried out in western Long Island Sound, NY, USA, a region that has experienced recent outbreaks of shellfish contaminated with V. parahaemolyticus. Pathogenic and non-pathogenic strains were quantified via PCR detection of marker genes and most probable number techniques. Field survey data showed little correspondence between fecal coliforms and V. parahaemolyticus, but significant correlations between V. parahaemolyticus and an alternative indicator, enterococci, and between V. parahaemolyticus and short-term (48 h) rainfall were observed. Experiments demonstrated that enrichment of seawater with phytoplankton-derived dissolved organic matter significantly increased the concentration of total V. parahaemolyticus and the presence pathogenic V. parahaemolyticus, but higher temperatures did not. Collectively, these study results suggest that fecal coliforms may fail to account for the full suite of important shellfish pathogens but that enterococci could provide a potential alternative or supplement to shellfish sanitation monitoring. Given the ability of algal-derived dissolved organic matter to promote the growth of pathogenic V. parahaemolyticus, restricting nutrient inputs into coastal water bodies that promote algal blooms may indirectly decrease the proliferation of V. parahaemolyticus and protect public health.

  19. MODELING FISH AND SHELLFISH DISTRIBUTIONS IN THE MOBILE BAY ESTUARY, USA

    EPA Science Inventory

    Estuaries in the Gulf of Mexico provide rich habitat for many fish and shellfish, including those that have been identified as economically and ecologically important. For the Mobile Bay estuary, we developed statistical models to relate distributions of individual species and sp...

  20. High pressure processing of bivalve shellfish and HPP's potential use as a virus intervention

    USDA-ARS?s Scientific Manuscript database

    Bivalve shellfish readily bioconcentrate pathogenic microbes and substance, such as algal and dinoflagulate toxins, fecal viruses and bacteria, and naturally present vibrio bacteria. High pressure processing (HPP) is currently used as an intervention for Vibrio vulnificus bacteria within molluscan ...

  1. Processing Strategies to Inactivate Enteric Viruses in Shellfish: Limitations of Surrogate Viruses and Molecular Methods

    USDA-ARS?s Scientific Manuscript database

    Noroviruses, hepatitis A and E viruses, sapovirus, astrovirus, rotavirus, Aichi virus, enteric adenoviruses, poliovirus, and other enteroviruses enter shellfish through contaminated seawater or by contamination during handling and processing, resulting in outbreaks ranging from isolated to epidemic....

  2. Discover the Atlantic Ocean: An Exciting Coloring Book of Fish and Shellfish.

    ERIC Educational Resources Information Center

    Flick, George J.

    This coloring book contains pictures of more than 79 fish and shellfish found on the Atlantic Coast. Captions give information on habitats, behavior, or commercial uses of the species pictured. Indexes of both common and scientific names are given. (BB)

  3. Spoilage and shelf-life extension of fresh fish and shellfish.

    PubMed

    Ashie, I N; Smith, J P; Simpson, B K

    1996-01-01

    Fresh fish and shellfish are highly perishable products due to their biological composition. Under normal refrigerated storage conditions, the shelf life of these products is limited by enzymatic and microbiological spoilage. However, with increasing consumer demands for fresh products with extended shelf life and increasing energy costs associated with freezing and frozen storage, the fish-processing industry is actively seeking alternative methods of shelf life preservation and marketability of fresh, refrigerated fish and at the same time economizing on energy costs. Additional methods that could fulfill these objectives include chemical decontamination, low-dose irradiation, ultra-high pressure, and modified atmosphere packaging (MAP). This review focuses on the biochemical and microbiological composition of fresh fish/shellfish, the spoilage patterns in these products, factors influencing spoilage, and the combination treatments that can be used in conjunction with refrigeration to extend the shelf life and keeping quality of fresh fish/shellfish. The safety concerns of minimally processed/MAP fish, specifically with respect to the growth of Clostridium botulinum type E, is also addressed.

  4. Determination of buildup and dilution of wastewater effluent in shellfish growing waters through a modified application of super-position.

    PubMed

    Goblick, Gregory N; Ao, Yaping; Anbarchian, Julie M; Calci, Kevin R

    2017-02-15

    Since 1925, dilution analysis has been used to minimize pathogenic impacts to bivalve molluscan shellfish growing areas from treated wastewater effluent in the National Shellfish Sanitation Program (NSSP). For over twenty five years, the U.S. Food and Drug Administration (FDA) has recommended a minimum of 1000:1 dilution of effluent within prohibited closure zones established around wastewater treatment plant (WWTP) discharges. During May 2010, using recent technologies, a hydrographic dye study was conducted in conjunction with a pathogen bioaccumulation study in shellfish adjacent to a WWTP discharge in Yarmouth, ME. For the first time an improved method of the super-position principle was used to determine the buildup of dye tagged sewage effluent and steady state dilution in tidal waters. Results of the improved method of dilution analysis illustrate an economical, reliable and more accurate and manageable approach for estimating the buildup and steady state pollutant conditions in coastal and estuarine waters. Published by Elsevier Ltd.

  5. Recruitment from an egg bank into the plankton in Baisha Bay, a mariculture base in Southern China

    NASA Astrophysics Data System (ADS)

    Wang, Qing; Luan, Lei-Lei; Chen, Liang-Dong; Yuan, Dan-Ni; Liu, Sheng; Hwang, Jiang-Shiou; Yang, Yu-Feng

    2016-11-01

    The potential recruitment of resting eggs of calanoid copepods and rotifers to planktonic populations was investigated in the surface and sub-surface sediments of three mariculture zones: an integrated seaweed Gracilaria lemaneiformis and shellfish cultivation area (G), a fish cultivation area (F), and a shellfish cultivation area (S), as well as the sediments of a nearby control sea area (C) in a mariculture base in Southern China. The potential recruitment of copepod and rotifer eggs in the sediments of C and G was significantly higher than in F and S. Potential recruitment in the sub-surface sediments of F and S was not observed, suggesting that fish and shellfish mariculture may be responsible for this decrease. The hatching success of resting eggs of copepods and rotifers was affected by mariculture type, and that large-scale seaweed cultivation may offset the adverse effect of fish and shellfish cultivation on the resting eggs if integrated cultivation is adopted.

  6. Risk assessment of the amnesic shellfish poison, domoic acid, on animals and humans.

    PubMed

    Kumar, K Prem; Kumar, Sreeletha Prem; Nair, G Achuthan

    2009-05-01

    Risk assessment of the amnesic shellfish poison, domoic acid, a potent neurotoxin, is evaluated based on its current knowledge and its harmful effects, and is presented under four headings, viz., (1) hazard identification, (2) dose response assessment, (3) exposure assessment and (4) risk characterization. Domoic acid binds the glutamate receptor site of the central nervous system (CNS) of humans and causes depolarization of neurons and an increase in cellularcalcium. In nature, domoic acid is produced by the algae, Pseudonitzschia spp. and they enter into the body of shellfish through their consumption. This toxin is reported to cause gastroenteritis, renal insufficiency confusion and memory loss in humans, since it affects the hippocampus of the brain. In rats, intraperitonial and oral administration of domoic acid result in scratching, tremor and convulsions, and in monkeys, the toxic symptoms like mastication, salivation, projectile vomiting, weakness, teeth grinding and lethargy are apparent. The no-observed-adverse-effect-level (NOAEL) in animals reveals that pure toxin is more effective than those isolated from shellfish. Based on LD50 values, it is found that intraperitonial administration of this toxin in animals is 31 fold more effective than oral administration. Low levels of domoic acid (0.20-0.75 ppm) show no toxic symptoms in non-human primates, but clinical effects are apparent in them and in humans, at a concentration of 1.0 ppm. The tolerable daily intake (TDI) of domoic acid for humans is calculated as 0.075 ppm, whereas for razor clams and crabs, the TDI are 19.4 and 31.5 ppm respectively. The hazard quotient (HQ) is found to be 2. Being an irreversible neurotoxin, domoic acid has severe public health implications. Death occurs in those above 68 years old. In order to ensure adequate protection to public health, the concentration of domoic acid in shellfish and shellfish parts at point of sale shall not exceed the current permissible limit of 20 microg g(-1) tissue. While processing shellfish, it maybe advisable to pay attention to factors such as environmental conditions, inter-organ variability in concentrations of domoic acid and cross contaminations.

  7. 7 CFR 301.38-4 - Interstate movement of regulated articles.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 5 2012-01-01 2012-01-01 false Interstate movement of regulated articles. 301.38-4...-4 Interstate movement of regulated articles. (a) Non-protected areas. Interstate movement of regulated articles into or through any State or area that is not designated as a protected area under § 301...

  8. 7 CFR 301.38-4 - Interstate movement of regulated articles.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 5 2011-01-01 2011-01-01 false Interstate movement of regulated articles. 301.38-4...-4 Interstate movement of regulated articles. (a) Non-protected areas. Interstate movement of regulated articles into or through any State or area that is not designated as a protected area under § 301...

  9. 75 FR 31747 - Notice of Request for Extension of Approval of an Information Collection; Interstate Movement of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-04

    ...] Notice of Request for Extension of Approval of an Information Collection; Interstate Movement of Certain... interstate movement of certain land tortoises. DATES: We will consider all comments that we receive on or... FURTHER INFORMATION CONTACT: For information on regulations for the interstate movement of certain land...

  10. 9 CFR 82.24 - Other interstate movements and special permits.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Other interstate movements and special... NEWCASTLE DIS- EASE (END) AND CHLAMYDIOSIS Chlamydiosis in Poultry § 82.24 Other interstate movements and special permits. (a) A special permit is required for the interstate movement of items whose movement...

  11. 78 FR 34635 - Notice of Request for Extension of Approval of an Information Collection; Interstate Movement of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-10

    ... Collection; Interstate Movement of Certain Land Tortoises AGENCY: Animal and Plant Health Inspection Service... with regulations for the interstate movement of certain land tortoises. DATES: We will consider all... regulations for the interstate movement of certain land tortoises, contact Dr. Christa Speekmann, Import...

  12. 40 CFR 81.18 - Metropolitan St. Louis Interstate Air Quality Control Region.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 17 2010-07-01 2010-07-01 false Metropolitan St. Louis Interstate Air... Air Quality Control Regions § 81.18 Metropolitan St. Louis Interstate Air Quality Control Region. The Metropolitan St. Louis Interstate Air Quality Control Region (Missouri-Illinois) is revised to consist of the...

  13. 75 FR 33174 - Approval and Promulgation of Implementation Plans; New Mexico; Interstate Transport of Pollution

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-11

    ... of interstate transport. In this rulemaking EPA is addressing only the requirement that pertains to... of, and extent of, interstate transport. In that action, EPA developed an approach that allowed the... technical analyses that EPA deemed useful to evaluate the issue of interstate transport on that geographic...

  14. 40 CFR 81.63 - Metropolitan Fort Smith Interstate Air Quality Control Region.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 17 2010-07-01 2010-07-01 false Metropolitan Fort Smith Interstate Air... Air Quality Control Regions § 81.63 Metropolitan Fort Smith Interstate Air Quality Control Region. The Metropolitan Fort Smith Interstate Air Quality Control Region (Arkansas-Oklahoma) has been revised to consist...

  15. 76 FR 81360 - Cooperative Inspection Programs: Interstate Shipment of Meat and Poultry Products; Correction

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-28

    .... FSIS-2008-0039] RIN 0583-AD37 Cooperative Inspection Programs: Interstate Shipment of Meat and Poultry... to ship meat and poultry products in interstate commerce. The final rule was published in the Federal... the Federal Register, the final rule, ``Cooperative Inspection Programs; Interstate Shipment of Meat...

  16. 40 CFR 81.86 - Metropolitan Sioux City Interstate Air Quality Control Region.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 17 2011-07-01 2011-07-01 false Metropolitan Sioux City Interstate Air... Air Quality Control Regions § 81.86 Metropolitan Sioux City Interstate Air Quality Control Region. The Metropolitan Sioux City Interstate Air Quality Control Region (Iowa-Nebraska-South Dakota) consists of the...

  17. 40 CFR 81.86 - Metropolitan Sioux City Interstate Air Quality Control Region.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 17 2010-07-01 2010-07-01 false Metropolitan Sioux City Interstate Air... Air Quality Control Regions § 81.86 Metropolitan Sioux City Interstate Air Quality Control Region. The Metropolitan Sioux City Interstate Air Quality Control Region (Iowa-Nebraska-South Dakota) consists of the...

  18. 16 CFR 240.6 - Interstate commerce.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ...(d) and 2(e) of the Act. (The commerce standard for sections 2 (d) and (e) is at least as inclusive... 16 Commercial Practices 1 2013-01-01 2013-01-01 false Interstate commerce. 240.6 Section 240.6... ALLOWANCES AND OTHER MERCHANDISING PAYMENTS AND SERVICES § 240.6 Interstate commerce. The term interstate...

  19. 16 CFR 240.6 - Interstate commerce.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ...(d) and 2(e) of the Act. (The commerce standard for sections 2 (d) and (e) is at least as inclusive... 16 Commercial Practices 1 2012-01-01 2012-01-01 false Interstate commerce. 240.6 Section 240.6... ALLOWANCES AND OTHER MERCHANDISING PAYMENTS AND SERVICES § 240.6 Interstate commerce. The term interstate...

  20. 16 CFR 240.6 - Interstate commerce.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ...(d) and 2(e) of the Act. (The commerce standard for sections 2 (d) and (e) is at least as inclusive... 16 Commercial Practices 1 2011-01-01 2011-01-01 false Interstate commerce. 240.6 Section 240.6... ALLOWANCES AND OTHER MERCHANDISING PAYMENTS AND SERVICES § 240.6 Interstate commerce. The term interstate...

  1. 16 CFR 240.6 - Interstate commerce.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ...(d) and 2(e) of the Act. (The commerce standard for sections 2 (d) and (e) is at least as inclusive... 16 Commercial Practices 1 2010-01-01 2010-01-01 false Interstate commerce. 240.6 Section 240.6... ALLOWANCES AND OTHER MERCHANDISING PAYMENTS AND SERVICES § 240.6 Interstate commerce. The term interstate...

  2. 29 CFR 782.7 - Interstate commerce requirements of exemption.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... interstate commerce). (Walling v. Craig, 53 F. Supp. 479 (D. Minn). See also Engbretson v. E. J. Albrecht Co... 29 Labor 3 2012-07-01 2012-07-01 false Interstate commerce requirements of exemption. 782.7... HOURS PROVISIONS FOR CERTAIN EMPLOYEES OF MOTOR CARRIERS § 782.7 Interstate commerce requirements of...

  3. 29 CFR 782.7 - Interstate commerce requirements of exemption.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... interstate commerce). (Walling v. Craig, 53 F. Supp. 479 (D. Minn). See also Engbretson v. E. J. Albrecht Co... 29 Labor 3 2010-07-01 2010-07-01 false Interstate commerce requirements of exemption. 782.7... HOURS PROVISIONS FOR CERTAIN EMPLOYEES OF MOTOR CARRIERS § 782.7 Interstate commerce requirements of...

  4. 16 CFR 240.6 - Interstate commerce.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ...(d) and 2(e) of the Act. (The commerce standard for sections 2 (d) and (e) is at least as inclusive... 16 Commercial Practices 1 2014-01-01 2014-01-01 false Interstate commerce. 240.6 Section 240.6... ALLOWANCES AND OTHER MERCHANDISING PAYMENTS AND SERVICES § 240.6 Interstate commerce. The term interstate...

  5. TRPV1 as a key determinant in ciguatera and neurotoxic shellfish poisoning

    PubMed Central

    Cuypers, Eva; Yanagihara, Angel; Rainier, Jon D.; Tytgat, Jan

    2007-01-01

    Ciguatera fish poisoning and neurotoxic shellfish poisoning are distinct clinical entities characterized by gastrointestinal and neurological disturbances, following the consumption of certain reef fish and shellfish containing toxic polyether compounds sporadically present in certain toxic marine dinoflagellates. The biotransformation and bioaccumulation of gambierol and brevetoxin, and their congeners, are believed to be involved in the pathogenesis of these “food-chain diseases”, for which no effective treatments are available. Here, we describe for the first time the potent effect of gambierol and brevetoxin on TRPV1 channels, a key player in thermal and pain sensation. Our findings may lead to promising new therapeutic interventions. PMID:17659256

  6. A simple, field-friendly technique for cryopreserving semen from Asian elephants (Elephas maximus).

    PubMed

    Arnold, Danielle M; Gray, Charlie; Roth, Terri L; Mitchell, Sebastian; Graham, Laura H

    2017-07-01

    The specific objectives of the present study were to investigate the effects of manual seeding, differing freeze and thaw rates as well as storage for 24h at 4°C prior to cryopreservation on post-thaw sperm quality in Asian elephants. Extended semen was cooled in an equitainer to 4°C, frozen in liquid nitrogen vapour at various rates with and without manual seeding or in a dry shipper and thawed at 37, 50 and 75°C. There was a significant effect of freeze rate on post-thaw motility (P<0.0001) and acrosomal integrity (P<0.005). The faster freeze rates in the dry shipper and at 1cm or 2cm above liquid nitrogen consistently provided better cryopreservation than slower freezing rates. Thaw temperature had no effect on post-thaw semen quality but there was an interaction between freeze and thaw rates with higher thaw rates resulting in superior post-thaw semen quality in straws frozen at fast rates. Storage of samples prior to freezing had a detrimental effect on post-thaw semen quality. In summary, our results indicate cooling extended semen in an equitainer and cryopreserving it by placing straws directly in a dry shipper is a simple technique for effectively cryopreserving Asian elephant semen in the field or zoo. Copyright © 2017 Elsevier B.V. All rights reserved.

  7. 21 CFR 1240.60 - Molluscan shellfish.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 8 2011-04-01 2011-04-01 false Molluscan shellfish. 1240.60 Section 1240.60 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) REGULATIONS UNDER CERTAIN OTHER ACTS ADMINISTERED BY THE FOOD AND DRUG ADMINISTRATION CONTROL OF COMMUNICABLE...

  8. TOXICITY ASSESSMENT OF PARALYTIC SHELLFISH POISONS (PSPS) USING QUANTITATIVE STRUCTURE-ACTIVITY RELATIONSHIPS

    EPA Science Inventory

    The most significant harmful algal bloom (HAB) toxin in terms of public health is commonly known as paralytic shellfish poisons (PSPs, "red tides" toxins). PSPs are neurotoxins produced by marine dinoflagellates and some cyanobacteria. PSPs comprise of over 21 natural toxins wi...

  9. PECONIC ESTUARY: AN ASSESSMENT OF SHELLFISH RESOURCES IN THE TRIBUTARIES AND EMBAYMENTS OF THE PECONIC ESTUARY

    EPA Science Inventory

    Executive Summary Historically, the Peconic Estuary's shellfish resources have supported significant fisheries for a number of species including hard clams, oysters and bay scallops. However, distribution and abundance data for the tributaries and embayments within the Peconic Es...

  10. 21 CFR 1240.60 - Molluscan shellfish.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Molluscan shellfish. 1240.60 Section 1240.60 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) REGULATIONS UNDER CERTAIN OTHER ACTS ADMINISTERED BY THE FOOD AND DRUG ADMINISTRATION CONTROL OF COMMUNICABLE...

  11. Potential virus detection and intervention methods for molluscan shellfish

    USDA-ARS?s Scientific Manuscript database

    Norovirus is the number one cause of foodborne illness in the Unites States, causing an estimated 9 million cases/yr. Hepatitis A is uncommon in the US but can result in serious illness. Bivalve shellfish are efficient bioconcentrators of these viruses from contaminated growing waters. Consequentl...

  12. Pulsed ultraviolet light reduces immunoglobulin E binding to atlantic white shrimp (litopenaeus setiferus).

    USDA-ARS?s Scientific Manuscript database

    To date, the only effective method to prevent allergic reactions to shellfish is complete avoidance; however, if processing methods could be employed to minimize shellfish allergens before products reach consumers, illness could be substantially lessened. Pulsed ultraviolet light (PUV), a novel food...

  13. Development and Validation of a Liquid Chromatography-Tandem Mass Spectrometry Method Coupled with Dispersive Solid-Phase Extraction for Simultaneous Quantification of Eight Paralytic Shellfish Poisoning Toxins in Shellfish.

    PubMed

    Yang, Xianli; Zhou, Lei; Tan, Yanglan; Shi, Xizhi; Zhao, Zhiyong; Nie, Dongxia; Zhou, Changyan; Liu, Hong

    2017-06-29

    In this study, a high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was developed for simultaneous determination of eight paralytic shellfish poisoning (PSP) toxins, including saxitoxin (STX), neosaxitoxin (NEO), gonyautoxins (GTX1-4) and the N -sulfo carbamoyl toxins C1 and C2, in sea shellfish. The samples were extracted by acetonitrile/water (80:20, v / v ) with 0.1% formic and purified by dispersive solid-phase extraction (dSPE) with C18 silica and acidic alumina. Qualitative and quantitative detection for the target toxins were conducted under the multiple reaction monitoring (MRM) mode by using the positive electrospray ionization (ESI) mode after chromatographic separation on a TSK-gel Amide-80 HILIC column with water and acetonitrile. Matrix-matched calibration was used to compensate for matrix effects. The established method was further validated by determining the linearity ( R ² ≥ 0.9900), average recovery (81.52-116.50%), sensitivity (limits of detection (LODs): 0.33-5.52 μg·kg -1 ; limits of quantitation (LOQs): 1.32-11.29 μg·kg -1 ) and precision (relative standard deviation (RSD) ≤ 19.10%). The application of this proposed approach to thirty shellfish samples proved its desirable performance and sufficient capability for simultaneous determination of multiclass PSP toxins in sea foods.

  14. Adenovirus and Norovirus Contaminants in Commercially Distributed Shellfish.

    PubMed

    Rodriguez-Manzano, Jesus; Hundesa, Ayalkibet; Calgua, Byron; Carratala, Anna; Maluquer de Motes, Carlos; Rusiñol, Marta; Moresco, Vanessa; Ramos, Ana Paula; Martínez-Marca, Fernando; Calvo, Miquel; Monte Barardi, Celia Regina; Girones, Rosina; Bofill-Mas, Sílvia

    2014-03-01

    Shellfish complying with European Regulations based on quantification of fecal bacterial indicators (FIB) are introduced into markets; however, information on viruses, more stable than FIB, is not available in the literature. To assess the presence of noroviruses (NoVs) GI and GII and human adenoviruses (HAdV) in domestic and imported mussels and clams (n = 151) their presence was analyzed during winter seasons (2004-2008) in north-west Spanish markets through a routine surveillance system. All samples tested negative for NoV GI and 13 % were positive for NoV GII. The role of HAdV as viral indicator was evaluated in 20 negative and 10 positive NoV GII samples showing an estimated sensitivity and specificity of HAdV to predict the presence of NoV GII of 100 and 74 % (cut-off 0.5). The levels of HAdV and NoVs and the efficiency of decontamination in shellfish depuration plants (SDP) were evaluated analyzing pre- and post-depurated mussels collected in May-June 2010 from three different SDP. There were no statistically significant differences in the prevalence and quantification of HAdV between pre- and post-depurated shellfish and between seawater entering and leaving the depuration systems. Moreover, infectious HAdV were detected in depurated mussels. These results confirm previous studies showing that current controls and depuration treatments limiting the number of FIB do not guarantee the absence of viruses in shellfish.

  15. Dose-Response Modelling of Paralytic Shellfish Poisoning (PSP) in Humans

    PubMed Central

    Arnich, Nathalie; Thébault, Anne

    2018-01-01

    Paralytic shellfish poisoning (PSP) is caused by a group of marine toxins with saxitoxin (STX) as the reference compound. Symptoms in humans after consumption of contaminated shellfish vary from slight neurological and gastrointestinal effects to fatal respiratory paralysis. A systematic review was conducted to identify reported cases of human poisoning associated with the ingestion of shellfish contaminated with paralytic shellfish toxins (PSTs). Raw data were collected from 143 exposed individuals (113 with symptoms, 30 without symptoms) from 13 studies. Exposure estimates were based on mouse bioassays except in one study. A significant relationship between exposure to PSTs and severity of symptoms was established by ordinal modelling. The critical minimal dose with a probability higher than 10% of showing symptoms is 0.37 µg STX eq./kg b.w. This means that 10% of the individuals exposed to this dose would have symptoms (without considering the severity of the symptoms). This dose is four-fold lower than the lowest-observed-adverse-effect-level (LOAEL) established by the European Food Safety Authority (EFSA, 2009) in the region of 1.5 μg STX eq./kg b.w. This work provides critical doses that could be used as point of departure to update the acute reference dose for STX. This is the first time a dose-symptoms model could be built for marine toxins using epidemiological data. PMID:29597338

  16. 7 CFR 301.75-7 - Interstate movement of regulated fruit from a quarantined area.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 5 2010-01-01 2010-01-01 false Interstate movement of regulated fruit from a... Citrus Canker Notice of Quarantine and Regulations § 301.75-7 Interstate movement of regulated fruit from... certificate for the interstate movement of the fruit in accordance with § 301.75-12. (b) Regulated fruit that...

  17. 7 CFR 301.75-8 - Interstate movement of regulated seed from a quarantined area.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 5 2011-01-01 2011-01-01 false Interstate movement of regulated seed from a... Citrus Canker Notice of Quarantine and Regulations § 301.75-8 Interstate movement of regulated seed from a quarantined area. Regulated seed may be moved interstate from a quarantined area into any area of...

  18. 7 CFR 301.75-8 - Interstate movement of regulated seed from a quarantined area.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 5 2010-01-01 2010-01-01 false Interstate movement of regulated seed from a... Citrus Canker Notice of Quarantine and Regulations § 301.75-8 Interstate movement of regulated seed from a quarantined area. Regulated seed may be moved interstate from a quarantined area into any area of...

  19. 76 FR 21835 - Approval and Promulgation of State Implementation Plans; State of Colorado; Interstate Transport...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-19

    ... another state's air quality through interstate transport. In this action, EPA is proposing to approve the Colorado Interstate Transport SIP provisions that address the requirement of section 110(a)(2)(D)(i) that... Interstate Transport SIP revision for the 1997 8-hour ozone and 1997 PM 2.5 NAAQS. This revision was...

  20. 9 CFR 82.5 - Interstate movement of live birds and live poultry from a quarantined area.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... (END) § 82.5 Interstate movement of live birds and live poultry from a quarantined area. (a) Pet birds. An individual may move his or her pet birds interstate from a quarantined area only if the birds are...) Epidemiological and testing requirements. For all pet birds moved interstate, epidemiological evidence must...

  1. 9 CFR 82.5 - Interstate movement of live birds and live poultry from a quarantined area.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... (END) § 82.5 Interstate movement of live birds and live poultry from a quarantined area. (a) Pet birds. An individual may move his or her pet birds interstate from a quarantined area only if the birds are...) Epidemiological and testing requirements. For all pet birds moved interstate, epidemiological evidence must...

  2. 78 FR 53269 - Approval and Promulgation of Implementation Plans; Arkansas; Interstate Transport of Fine...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-29

    ...] Approval and Promulgation of Implementation Plans; Arkansas; Interstate Transport of Fine Particulate... technical supplement submitted on March 20, 2013, to address interstate transport for the 1997 and 2006 PM 2... SIP'' is amended by adding an entry at the end for ``Interstate transport for the 1997 and 2006 PM 2.5...

  3. 75 FR 61354 - Safety Zone; Interstate 5 Bridge Repairs, Columbia River, Portland, OR

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-05

    ...-AA00 Safety Zone; Interstate 5 Bridge Repairs, Columbia River, Portland, OR AGENCY: Coast Guard, DHS... waters of the Columbia River due to repairs being made to the Interstate 5 Bridge. The safety zone is... would be contrary to public interest since the repairs to the Interstate 5 Bridge would be completed by...

  4. RELATIONS OF FISH AND SHELLFISH DISTRIBUTIONS TO HABITAT AND WATER QUALITY IN THE MOBILE BAY ESTUARY, USA

    EPA Science Inventory

    The Mobile Bay estuary provides rich habitat for many fish and shellfish, including those identified as economically and ecologically important. The National Estuary Program has focused on restoration of degraded estuarine habitat on which these species depend. To support this ...

  5. Prevalence and antimicrobial susceptibility of Vibrio parahaemolyticus isolated from retail shellfish in Shanghai

    USDA-ARS?s Scientific Manuscript database

    Vibrio parahaemolyticus is a marine and estuarine bacterium that poses a large threat to human health worldwide. It has been the leading bacterial cause of seafood-borne illness. This study investigated the prevalence and drug resistance of V. parahaemolyticus isolated from retail shellfish in Shang...

  6. 34 CFR 200.81 - Program definitions.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... processing of fish or shellfish or the raising or harvesting of fish or shellfish at fish farms. It consists... means a person who, in the preceding 36 months, has moved, as defined in paragraph (g), from one school... single school district, in order to obtain temporary employment or seasonal employment in agricultural...

  7. 34 CFR 200.81 - Program definitions.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... processing of fish or shellfish or the raising or harvesting of fish or shellfish at fish farms. It consists... means a person who, in the preceding 36 months, has moved, as defined in paragraph (g), from one school... single school district, in order to obtain temporary employment or seasonal employment in agricultural...

  8. 34 CFR 200.81 - Program definitions.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... processing of fish or shellfish or the raising or harvesting of fish or shellfish at fish farms. It consists... means a person who, in the preceding 36 months, has moved, as defined in paragraph (g), from one school... single school district, in order to obtain temporary employment or seasonal employment in agricultural...

  9. 34 CFR 200.81 - Program definitions.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... processing of fish or shellfish or the raising or harvesting of fish or shellfish at fish farms. It consists... means a person who, in the preceding 36 months, has moved, as defined in paragraph (g), from one school... single school district, in order to obtain temporary employment or seasonal employment in agricultural...

  10. 34 CFR 200.81 - Program definitions.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... processing of fish or shellfish or the raising or harvesting of fish or shellfish at fish farms. It consists... means a person who, in the preceding 36 months, has moved, as defined in paragraph (g), from one school... single school district, in order to obtain temporary employment or seasonal employment in agricultural...

  11. New perspectives on virus detection in shellfish: hemocytes as a source of concentrated virus

    USDA-ARS?s Scientific Manuscript database

    USDA ARS research indicates that circulating phagocytic cells (hemocytes) within oysters retain virus particles. We find that persistence of hepatitis A virus (HAV) within oyster hemocytes correlates with the presence of virus within whole oysters. Since bivalve shellfish have no self-nonself immun...

  12. High pressure processing as an intervention for raw virus-contaminated shellfish

    USDA-ARS?s Scientific Manuscript database

    Over the past 7 years, the USDA ARS Seafood Safety Laboratory has evaluated the potential use of high pressure processing (HPP) as a processing strategy for virus-contaminated shellfish. HPP can inactivate hepatitis A virus, (HAV), the human norovirus surrogates feline calicivirus and murine norovi...

  13. Determination of paralytic shellfish toxins in shellfish by receptor binding assay: collaborative study.

    PubMed

    Van Dolah, Frances M; Fire, Spencer E; Leighfield, Tod A; Mikulski, Christina M; Doucette, Gregory J

    2012-01-01

    A collaborative study was conducted on a microplate format receptor binding assay (RBA) for paralytic e shellfish toxins (PST). The assay quantifies the composite PST toxicity in shellfish samples based on the ability of sample extracts to compete with (3)H saxitoxin (STX) diHCl for binding to voltage-gated sodium channels in a rat brain membrane preparation. Quantification of binding can be carried out using either a microplate or traditional scintillation counter; both end points were included in this study. Nine laboratories from six countries completed the study. One laboratory analyzed the samples using the precolumn oxidation HPLC method (AOAC Method 2005.06) to determine the STX congener composition. Three laboratories performed the mouse bioassay (AOAC Method 959.08). The study focused on the ability of the assay to measure the PST toxicity of samples below, near, or slightly above the regulatory limit of 800 (microg STX diHCl equiv./kg). A total of 21 shellfish homogenates were extracted in 0.1 M HCl, and the extracts were analyzed by RBA in three assays on separate days. Samples included naturally contaminated shellfish samples of different species collected from several geographic regions, which contained varying STX congener profiles due to their exposure to different PST-producing dinoflagellate species or differences in toxin metabolism: blue mussel (Mytilus edulis) from the U.S. east and west coasts, California mussel (Mytilus californianus) from the U.S. west coast, chorito mussel (Mytilus chiliensis) from Chile, green mussel (Perna canaliculus) from New Zealand, Atlantic surf clam (Spisula solidissima) from the U.S. east coast, butter clam (Saxidomus gigantea) from the west coast of the United States, almeja clam (Venus antiqua) from Chile, and Atlantic sea scallop (Plactopecten magellanicus) from the U.S. east coast. All samples were provided as whole animal homogenates, except Atlantic sea scallop and green mussel, from which only the hepatopancreas was homogenized. Among the naturally contaminated samples, five were blind duplicates used for calculation of RSDr. The interlaboratory RSDR of the assay for 21 samples tested in nine laboratories was 33.1%, yielding a HorRat value of 2.0. Removal of results for one laboratory that reported systematically low values resulted in an average RSDR of 28.7% and average HorRat value of 1.8. Intralaboratory RSDr based on five blind duplicate samples tested in separate assays, was 25.1%. RSDr obtained by individual laboratories ranged from 11.8 to 34.9%. Laboratories that are routine users of the assay performed better than nonroutine users, with an average RSDr of 17.1%. Recovery of STX from spiked shellfish homogenates was 88.1-93.3%. Correlation with the mouse bioassay yielded a slope of 1.64 and correlation coefficient (r(2)) of 0.84, while correlation with the precolumn oxidation HPLC method yielded a slope of 1.20 and an r(2) of 0.92. When samples were sorted according to increasing toxin concentration (microg STX diHCl equiv./kg) as assessed by the mouse bioassay, the RBA returned no false negatives relative to the 800 microg STX diHCl equiv./kg regulatory limit for shellfish. Currently, no validated methods other than the mouse bioassay directly measure a composite toxic potency for PST in shellfish. The results of this interlaboratory study demonstrate that the RBA is suitable for the routine determination of PST in shellfish in appropriately equipped laboratories.

  14. 9 CFR 78.2 - Handling of certificates, permits, and “S” brand permits for interstate movement of animals.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... âSâ brand permits for interstate movement of animals. 78.2 Section 78.2 Animals and Animal Products... certificates, permits, and “S” brand permits for interstate movement of animals. (a) Any certificate, permit, or “S” brand permit required by this part for the interstate movement of animals shall be delivered...

  15. 75 FR 56935 - Approval and Promulgation of State Implementation Plans; State of Colorado; Interstate Transport...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-17

    ... Implementation Plan to Meet the Requirements of Clean Air Act section 110(a)(2)(D)(i)(I)--Interstate Transport... section 110(a)(2)(D)(i)(I). On June 18, 2009 the State of Colorado submitted an interstate transport State Implementation Plan (SIP) addressing the interstate transport requirements under section 110(a)(2)(D)(i) of the...

  16. 23 CFR 661.49 - Can IRRBP funds be spent on Interstate, State Highway, and Toll Road IRR bridges?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 23 Highways 1 2012-04-01 2012-04-01 false Can IRRBP funds be spent on Interstate, State Highway, and Toll Road IRR bridges? 661.49 Section 661.49 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT... Can IRRBP funds be spent on Interstate, State Highway, and Toll Road IRR bridges? Yes. Interstate...

  17. 23 CFR 661.49 - Can IRRBP funds be spent on Interstate, State Highway, and Toll Road IRR bridges?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 23 Highways 1 2014-04-01 2014-04-01 false Can IRRBP funds be spent on Interstate, State Highway, and Toll Road IRR bridges? 661.49 Section 661.49 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT... Can IRRBP funds be spent on Interstate, State Highway, and Toll Road IRR bridges? Yes. Interstate...

  18. 23 CFR 661.49 - Can IRRBP funds be spent on Interstate, State Highway, and Toll Road IRR bridges?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 23 Highways 1 2013-04-01 2013-04-01 false Can IRRBP funds be spent on Interstate, State Highway, and Toll Road IRR bridges? 661.49 Section 661.49 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT... Can IRRBP funds be spent on Interstate, State Highway, and Toll Road IRR bridges? Yes. Interstate...

  19. 19 CFR 122.76 - Shipper's Export Declarations and inspection certificates.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... SECURITY; DEPARTMENT OF THE TREASURY AIR COMMERCE REGULATIONS Documents Required for Clearance and Permission To Depart; Electronic Manifest Requirements for Passengers, Crew Members, and Non-Crew Members...

  20. 49 CFR 212.109 - Joint planning of inspections.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION STATE SAFETY PARTICIPATION REGULATIONS State/Federal Roles § 212.109... designed to monitor the compliance of the railroads, shippers, and manufacturers operating within the State...

  1. Floodflow characteristics of Filbin Creek at proposed interstate highway 526, North Charleston, South Carolina

    USGS Publications Warehouse

    Bohman, L.R.

    1984-01-01

    A study to determine the impact of two alternative construction plans for proposed interchange between the existing Interstate Highway 26 and Interstate Highway 526 in the Filbin Creek drainage basin near North Charleston, South Carolina was performed by the U.S. Geological Survey, in cooperation with the South Carolina Department of Highways and Public Transportation. A computerized reservoir routing technique was used to route synthetic flood hydrographs through the basin system. Simulation results indicate that the new roadway will cause little or no change in water-surface elevations downstream of Interstate Highway 26. Upstream of Interstate Highway 26, approximately 0.5 foot of backwater will be created by either alternative during a 100-year flood as a result of the Interstate Highway 526 embankments and structures. (USGS)

  2. 2017 SmartWay Shipper Tool Demonstration

    EPA Pesticide Factsheets

    This EPA presentation provides information on the SmartWay Logistics Carrier Tool: its background and development, participation in the program, application process, emission metrics, tool demonstration, data collection, and schedule for 2017.

  3. 9 CFR 82.5 - Interstate movement of live birds and live poultry from a quarantined area.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 9 Animals and Animal Products 1 2011-01-01 2011-01-01 false Interstate movement of live birds and... (END) § 82.5 Interstate movement of live birds and live poultry from a quarantined area. (a) Pet birds. An individual may move his or her pet birds interstate from a quarantined area only if the birds are...

  4. 9 CFR 82.5 - Interstate movement of live birds and live poultry from a quarantined area.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Interstate movement of live birds and... (END) § 82.5 Interstate movement of live birds and live poultry from a quarantined area. (a) Pet birds. An individual may move his or her pet birds interstate from a quarantined area only if the birds are...

  5. 49 CFR 350.337 - How may State laws and regulations governing motor carriers, CMV drivers, and CMVs in interstate...

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... carriers, CMV drivers, and CMVs in interstate commerce differ from the FMCSRs and still be considered... regulations governing motor carriers, CMV drivers, and CMVs in interstate commerce differ from the FMCSRs and... through E and H of part 107, and §§ 171.15 and 171.16, as applicable to either interstate or intrastate...

  6. 49 CFR 350.337 - How may State laws and regulations governing motor carriers, CMV drivers, and CMVs in interstate...

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... carriers, CMV drivers, and CMVs in interstate commerce differ from the FMCSRs and still be considered... regulations governing motor carriers, CMV drivers, and CMVs in interstate commerce differ from the FMCSRs and... through E and H of part 107, and §§ 171.15 and 171.16, as applicable to either interstate or intrastate...

  7. 49 CFR 350.337 - How may State laws and regulations governing motor carriers, CMV drivers, and CMVs in interstate...

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... carriers, CMV drivers, and CMVs in interstate commerce differ from the FMCSRs and still be considered... regulations governing motor carriers, CMV drivers, and CMVs in interstate commerce differ from the FMCSRs and... through E and H of part 107, and §§ 171.15 and 171.16, as applicable to either interstate or intrastate...

  8. 49 CFR 350.337 - How may State laws and regulations governing motor carriers, CMV drivers, and CMVs in interstate...

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... carriers, CMV drivers, and CMVs in interstate commerce differ from the FMCSRs and still be considered... regulations governing motor carriers, CMV drivers, and CMVs in interstate commerce differ from the FMCSRs and... through E and H of part 107, and §§ 171.15 and 171.16, as applicable to either interstate or intrastate...

  9. 49 CFR 350.337 - How may State laws and regulations governing motor carriers, CMV drivers, and CMVs in interstate...

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... carriers, CMV drivers, and CMVs in interstate commerce differ from the FMCSRs and still be considered... regulations governing motor carriers, CMV drivers, and CMVs in interstate commerce differ from the FMCSRs and... through E and H of part 107, and §§ 171.15 and 171.16, as applicable to either interstate or intrastate...

  10. 7 CFR 60.128 - United States country of origin.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 3 2011-01-01 2011-01-01 false United States country of origin. 60.128 Section 60.128... FOR FISH AND SHELLFISH General Provisions Definitions § 60.128 United States country of origin. United...: From fish or shellfish hatched, raised, harvested, and processed in the United States, and that has not...

  11. 7 CFR 60.128 - United States country of origin.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 3 2012-01-01 2012-01-01 false United States country of origin. 60.128 Section 60.128... FOR FISH AND SHELLFISH General Provisions Definitions § 60.128 United States country of origin. United...: From fish or shellfish hatched, raised, harvested, and processed in the United States, and that has not...

  12. UTILIZING SHELLFISH RESPONSES TO SET TARGET WATER QUALITY CONDITIONS FOR THE RESTORATION OF OYSTER REEFS IN THE CALOOSAHATCHEE ESTUARY, FLORIDA.

    EPA Science Inventory

    Volety, Aswani K., S.G. Tolley and James T. Winstead. 2002. Utilizing Shellfish Responses to Set Target Water Quality Conditions for the Restoration of Oyster Reefs in the Caloosahatchee Estuary, Florida. Presented at the International Workshop on Restoration of Benthic Invertebr...

  13. Fluorogenic membrane overlays to enumerate total coliforms, Escherichia coli, and total Vibrionaceae in shellfish and seawater

    USDA-ARS?s Scientific Manuscript database

    Three assays were developed to enumerate total coliforms, Escherichia coli, and total Vibrionaceae in shellfish and other foods and in seawater and other environmental samples. Assays involve membrane overlays of overnight colonies on non-selective agar plates to detect ß-glucuronidase and lysyl am...

  14. 40 CFR 125.73 - Criteria and standards for the determination of alternative effluent limitations under section...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... than necessary to assure the protection and propagation of a balanced, indigenous community of... affected, will assure the protection and propagation of a balanced indigenous community of shellfish, fish... thermal sources to a balanced, indigenous community of shellfish, fish and wildlife in and on the body of...

  15. 40 CFR 125.73 - Criteria and standards for the determination of alternative effluent limitations under section...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... than necessary to assure the protection and propagation of a balanced, indigenous community of... affected, will assure the protection and propagation of a balanced indigenous community of shellfish, fish... thermal sources to a balanced, indigenous community of shellfish, fish and wildlife in and on the body of...

  16. 40 CFR 125.73 - Criteria and standards for the determination of alternative effluent limitations under section...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... than necessary to assure the protection and propagation of a balanced, indigenous community of... affected, will assure the protection and propagation of a balanced indigenous community of shellfish, fish... thermal sources to a balanced, indigenous community of shellfish, fish and wildlife in and on the body of...

  17. 40 CFR 125.73 - Criteria and standards for the determination of alternative effluent limitations under section...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... than necessary to assure the protection and propagation of a balanced, indigenous community of... affected, will assure the protection and propagation of a balanced indigenous community of shellfish, fish... thermal sources to a balanced, indigenous community of shellfish, fish and wildlife in and on the body of...

  18. 40 CFR 125.73 - Criteria and standards for the determination of alternative effluent limitations under section...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... than necessary to assure the protection and propagation of a balanced, indigenous community of... affected, will assure the protection and propagation of a balanced indigenous community of shellfish, fish... thermal sources to a balanced, indigenous community of shellfish, fish and wildlife in and on the body of...

  19. DOE Office of Scientific and Technical Information (OSTI.GOV)

    NONE

    Trawl surveys were conducted in Prince William Sound and adjacent waters in 1989 and 1990 to assess impacts of the Exxon Valdex oil spill on commercial species of bottomfish and shellfish. The surveys (1) determined abundance, distribution, and year-class strength of important bottomfish and shellfish species and (2) assessed the incidence and distribution of oil contamination in fish bile.

  20. Human Health Baseline Risk Assessment for the Estuary, Operable Unit 1, Marsh Trespasser, Fish and Shellfish Consumer, Clapper Rail Consumer, LCP Chemicals Superfund Site

    EPA Pesticide Factsheets

    report on marsh trespasser and consumption of fish, shellfish, and clapper rail at LCP Superfund site in Brunswick, Georgia, prepared by EPS, Inc. in Aug. 2011. Region ID: 04 DocID: 10841008, DocDate: 08-01-2011

  1. Vitamin D levels in fish and shellfish determined by liquid chromatography with ultraviolet detection and mass spectrometry

    USDA-ARS?s Scientific Manuscript database

    Vitamin D3 (cholecalciferol) levels were determined in finfish and shellfish using UV detection at 265nm (combined with auxiliary full scan UV detection) and selected ion monitoring (SIM) mass spectrometry (MS), using vitamin D2 (ergocalciferol) as an internal standard. Analysis of standard referen...

  2. Effect of copper on the scope for growth of clams (Tapes philippinarum) from a farming area in the Northern Adriatic Sea.

    PubMed

    Munari, Cristina; Mistri, Michele

    2007-09-01

    Copper is currently the most common biocide in antifouling paints. Levels of this metal were measured in the water, particulate matter and sediments from a shellfish farming area in the Sacca di Goro (Northern Adriatic Sea) over one year. With respect to the 1980s, copper environmental level increased twofold. The release of copper from shellfish farmers' boats was also estimated to be > 250 kg Cu y(-1). Clams Tapes philippinarum were collected in the same area and seasonally exposed to a sublethal (10 microg Cu l(-1)) concentration of copper. Physiological traits were significantly affected by copper exposure (scope for growth declined as a result of reduced clearance rate, increased oxygen consumption and a generally lower absorption efficiency). The results of this study are cause for concern for shellfish farming activities at least in Northern Adriatic, where shellfish farming is a monoculture of T. philippinarum. A strict interpretation of the precautionary principle might suggest that more rigorous regulatory action to control copper inputs in the field would be justified.

  3. Evaluating shellfish gathering ( Lucina pectinata) in a tropical mangrove system

    NASA Astrophysics Data System (ADS)

    Rondinelli, S. F.; Barros, F.

    2010-10-01

    Fish resources are important sources of income and protein to traditional inhabitants of coastal zones. In Garapuá village, the shellfish Lucina pectinata is the main resource exploited in mangroves. This study tests whether if in less explored areas (far from the village) L. pectinata individuals have higher densities and greater lengths, and if there was a decrease in cpue's over the last years. Samples were taken monthly in two habitats (mangrove channels and mangrove roots) in six mangrove areas by random squares. The results indicated that closer areas showed significantly lower densities than areas far from the village. Densities were significantly higher in mangrove roots (quizangas) than at channels. There was a significant increase in monthly L. pectinata cpue, from 18.2 dz./shellfish gatherers/day in 2001 to 19.3 in 2007, showing that this stock does not seem to be overexploited. However, (i) a long-term monitoring of Garapuá shellfish gatherers to evaluate if the stock will support an increasing pressure and (ii) several manipulative experiments to better understand ecological processes are suggested.

  4. Closer view looking to the Interstate Commerce Commission building and ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Closer view looking to the Interstate Commerce Commission building and Ariel Rios Federal building - Interstate Commerce Commission, Constitution Avenue between Twelfth and Fourteenth streets, Washington, District of Columbia, DC

  5. Development and Validation of a Liquid Chromatography-Tandem Mass Spectrometry Method Coupled with Dispersive Solid-Phase Extraction for Simultaneous Quantification of Eight Paralytic Shellfish Poisoning Toxins in Shellfish

    PubMed Central

    Yang, Xianli; Zhou, Lei; Tan, Yanglan; Shi, Xizhi; Zhao, Zhiyong; Nie, Dongxia; Zhou, Changyan; Liu, Hong

    2017-01-01

    In this study, a high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was developed for simultaneous determination of eight paralytic shellfish poisoning (PSP) toxins, including saxitoxin (STX), neosaxitoxin (NEO), gonyautoxins (GTX1–4) and the N-sulfo carbamoyl toxins C1 and C2, in sea shellfish. The samples were extracted by acetonitrile/water (80:20, v/v) with 0.1% formic and purified by dispersive solid-phase extraction (dSPE) with C18 silica and acidic alumina. Qualitative and quantitative detection for the target toxins were conducted under the multiple reaction monitoring (MRM) mode by using the positive electrospray ionization (ESI) mode after chromatographic separation on a TSK-gel Amide-80 HILIC column with water and acetonitrile. Matrix-matched calibration was used to compensate for matrix effects. The established method was further validated by determining the linearity (R2 ≥ 0.9900), average recovery (81.52–116.50%), sensitivity (limits of detection (LODs): 0.33–5.52 μg·kg−1; limits of quantitation (LOQs): 1.32–11.29 μg·kg−1) and precision (relative standard deviation (RSD) ≤ 19.10%). The application of this proposed approach to thirty shellfish samples proved its desirable performance and sufficient capability for simultaneous determination of multiclass PSP toxins in sea foods. PMID:28661471

  6. Bivalve molluscs as vectors of marine biotoxins involved in seafood poisoning.

    PubMed

    Ciminiello, P; Fattorusso, E

    2006-01-01

    Molluscs of many sorts, which are high in protein and trace minerals, have always been a substantial portion of the human diet. A great variety of mollusc species are therefore of commercial importance throughout the world. Episodes of poisoning occasionally happen to the consumers of molluscs, the main hazard being represented by bivalve molluscs. These organisms are filter-feeders, feeding mainly on a wide range of phytoplankton species. Among the thousands of species of microscopic algae at the base of the marine food chain, there are a few dozen which produce potent toxins. One major category of impact occurs when toxic phytoplankton are filtered from the water as food by shellfish, which then accumulate the algal toxins to levels which can be lethal to humans. Incidences of poisoning related to marine algal toxins come under the main categories of paralytic shellfish poisoning (PSP), neurotoxic shellfish poisoning (NSP), diarrhetic shellfish poisoning (DSP), and amnesic shellfish poisoning (ASP), depending upon the toxins and the symptoms that they cause. Since the beginning of the 1990s, a research program has been initiated to examine the toxin profiles in mussels from the Adriatic Sea. Since then, a number of polyether toxins have been isolated and characterized, some of which represent new additions to the DSP class of biotoxins. During this investigation, new types of toxins have also been isolated. The recent application of LC-MS methods for the detection of Adriatic marine biotoxins made it possible to speed up the analysis of toxic samples.

  7. 40 CFR 81.319 - Louisiana.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Parish West Carroll Parish AQCR 022 Shreveport-Texarkana-Tyler Interstate Unclassifiable/Attainment... Parish AQCR 022 Shreveport-Texarkana-Tyler Interstate Unclassifiable/Attainment Avoyelles Parish... Parish Union Parish West Carroll Parish AQCR 022 Shreveport-Texarkana-Tyler Interstate Unclassifiable...

  8. How the SmartWay Partnership Works

    EPA Pesticide Factsheets

    This page describes how the SmartWay program and the SmartWay Transport Partnership work for carriers, shippers, and logistics companies to track air quality, reduce fuel consumption, improve freight supply chain sustainability.

  9. 76 FR 22449 - Release of Waybill Data

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-21

    ... Office of Economics. The waybill sample contains confidential railroad and shipper data; [[Page 22450... the Board's Office of Economics within 14 calendar days of the date of this notice. The rules for...

  10. 50 CFR 14.109 - Care in transit.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... veterinary care if no veterinary attendant is traveling with the shipment; if the shipper cannot be reached in the case of an emergency, qualified veterinary care should be provided. A veterinarian or...

  11. 50 CFR 14.109 - Care in transit.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... veterinary care if no veterinary attendant is traveling with the shipment; if the shipper cannot be reached in the case of an emergency, qualified veterinary care should be provided. A veterinarian or...

  12. Columbia Terminal Railroad (COLT) feasibility analysis

    DOT National Transportation Integrated Search

    2009-06-01

    The Missouri Department of Transportation partnered with public agencies and a private company to determine expansion feasibility of intermodal freight movement through the Columbia Terminal Railroad (COLT) in Central Missouri. Businesses and shipper...

  13. Maternal fish and shellfish consumption and wheeze, eczema and food allergy at age two: a prospective cohort study in Brittany, France.

    PubMed

    Pelé, Fabienne; Bajeux, Emma; Gendron, Hélène; Monfort, Christine; Rouget, Florence; Multigner, Luc; Viel, Jean-François; Cordier, Sylvaine

    2013-12-02

    Environmental exposures, including dietary contaminants, may influence the developing immune system. This study assesses the association between maternal pre-parturition consumption of seafood and wheeze, eczema, and food allergy in preschool children. Fish and shellfish were studied separately as they differ according to their levels of omega-3 polyunsaturated fatty acids (which have anti-allergic properties) and their levels of contaminants. The PELAGIE cohort included 3421 women recruited at the beginning of pregnancy. Maternal fish and shellfish intake was measured at inclusion by a food frequency questionnaire. Wheeze, eczema, and food allergy were evaluated by a questionnaire completed by the mother when the child was 2 years old (n = 1500). Examination of the associations between seafood intake and outcomes took major confounders into account. Complementary sensitivity analyses with multiple imputation enabled us to handle missing data, due mostly to attrition. Moderate maternal pre-parturition fish intake (1 to 4 times a month) was, at borderline significance, associated with a lower risk of wheeze (adjusted OR = 0.69 (0.45-1.05)) before age 2, compared with low intake (< once/month). This result was not, however, consistent: after multiple imputation, the adjusted OR was 0.86 (0.63-1.17). Shellfish intake at least once a month was associated with a higher risk of food allergy before age 2 (adjusted OR = 1.62 (1.11-2.37)) compared to low or no intake (< once/month). Multiple imputation confirmed this association (adjusted OR = 1.52 (1.05-2.21)). This study suggests that maternal pre-parturition shellfish consumption may increase the risk of food allergy. Further large-scale epidemiologic studies are needed to corroborate these results, identify the contaminants or components of shellfish responsible for the effects observed, determine the persistence of the associations seen at age 2, and investigate potential associations with health effects observable at later ages, such as allergic asthma.

  14. Shellfish/crustacean oral allergy syndrome among national service pre-enlistees in Singapore

    PubMed Central

    Arulanandam, Shalini; Tan, Sze-Chin; Tan, Teck-Choon; Chan, Grace Yin-Lai; Tan, Justina Wei-Lyn; Yeow, Mark Chong-Wei; Tang, Chwee-Ying; Hou, Jinfeng; Leong, Khai-Pang

    2018-01-01

    Background All Singaporean males undergo medical screening prior to compulsory military service. A history of possible food allergy may require referral to a specialist Allergy clinic to ensure that special dietary needs can be taken into account during field training and deployment. Objective To study the pattern of food allergy among pre-enlistees who were referred to a specialist allergy clinic to work up suspected food allergy. Methods Retrospective study of all pre-enlistees registered in the Clinical Immunology/Allergy New Case Registry referred to the Allergy Clinic from 1 August 2015 to 31 May 2016 for suspected food allergy. Results One hundred twenty pre-enlistees reporting food allergy symptoms other than rash alone were referred to the Allergy Clinic during the study period. Of these, 77 (64.2%) had food allergy. Among those with food allergy, mean age was 19.1 ± 1.5 years. They comprised predominantly Chinese (66.2%) and Malays (20.8%). The most commonly reported foods were shellfish/crustaceans (78%), peanut (15.6%), and egg (6.5%). Self-limiting oral allergy syndrome, OAS (itchy lips and throat with/without lip angioedema) was the most common manifestation (n = 33, 42.9%) followed by anaphylaxis (n = 23, 29.9%). Majority of OAS was from shellfish/crustacean (90.6%); of which shrimp (30.3%), crab (15.2%), and lobster (3.0%) were the most common. Mild childhood asthma (69.7%), allergic rhinitis (6.3%), and eczema (6.1%) were the most common atopic conditions among individuals with shellfish/crustacean OAS. This pattern was similar for shellfish/crustacean anaphylaxis. Skin prick tests were most commonly positive for shrimp (OAS 87.1% vs. anaphylaxis 100%), crab (OAS 95.8% vs. 90.9%), and lobster (OAS 91.7% vs. 63.6%). Conclusion OAS to shellfish/crustaceans was more common than anaphylaxis among this study population of young males referred for food allergy symptoms other than rash alone. PMID:29732294

  15. Toxic marine microalgae and shellfish poisoning in the British isles: history, review of epidemiology, and future implications

    PubMed Central

    2011-01-01

    The relationship between toxic marine microalgae species and climate change has become a high profile and well discussed topic in recent years, with research focusing on the possible future impacts of changing hydrological conditions on Harmful Algal Bloom (HAB) species around the world. However, there is very little literature concerning the epidemiology of these species on marine organisms and human health. Here, we examine the current state of toxic microalgae species around the UK, in two ways: first we describe the key toxic syndromes and gather together the disparate reported data on their epidemiology from UK records and monitoring procedures. Secondly, using NHS hospital admissions and GP records from Wales, we attempt to quantify the incidence of shellfish poisoning from an independent source. We show that within the UK, outbreaks of shellfish poisoning are rare but occurring on a yearly basis in different regions and affecting a diverse range of molluscan shellfish and other marine organisms. We also show that the abundance of a species does not necessarily correlate to the rate of toxic events. Based on routine hospital records, the numbers of shellfish poisonings in the UK are very low, but the identification of the toxin involved, or even a confirmation of a poisoning event is extremely difficult to diagnose. An effective shellfish monitoring system, which shuts down aquaculture sites when toxins exceed regularity limits, has clearly prevented serious impact to human health, and remains the only viable means of monitoring the potential threat to human health. However, the closure of these sites has an adverse economic impact, and the monitoring system does not include all toxic plankton. The possible geographic spreading of toxic microalgae species is therefore a concern, as warmer waters in the Atlantic could suit several species with southern biogeographical affinities enabling them to occupy the coastal regions of the UK, but which are not yet monitored or considered to be detrimental. PMID:21645342

  16. Shellfish/crustacean oral allergy syndrome among national service pre-enlistees in Singapore.

    PubMed

    Thong, Bernard Yu-Hor; Arulanandam, Shalini; Tan, Sze-Chin; Tan, Teck-Choon; Chan, Grace Yin-Lai; Tan, Justina Wei-Lyn; Yeow, Mark Chong-Wei; Tang, Chwee-Ying; Hou, Jinfeng; Leong, Khai-Pang

    2018-04-01

    All Singaporean males undergo medical screening prior to compulsory military service. A history of possible food allergy may require referral to a specialist Allergy clinic to ensure that special dietary needs can be taken into account during field training and deployment. To study the pattern of food allergy among pre-enlistees who were referred to a specialist allergy clinic to work up suspected food allergy. Retrospective study of all pre-enlistees registered in the Clinical Immunology/Allergy New Case Registry referred to the Allergy Clinic from 1 August 2015 to 31 May 2016 for suspected food allergy. One hundred twenty pre-enlistees reporting food allergy symptoms other than rash alone were referred to the Allergy Clinic during the study period. Of these, 77 (64.2%) had food allergy. Among those with food allergy, mean age was 19.1 ± 1.5 years. They comprised predominantly Chinese (66.2%) and Malays (20.8%). The most commonly reported foods were shellfish/crustaceans (78%), peanut (15.6%), and egg (6.5%). Self-limiting oral allergy syndrome, OAS (itchy lips and throat with/without lip angioedema) was the most common manifestation (n = 33, 42.9%) followed by anaphylaxis (n = 23, 29.9%). Majority of OAS was from shellfish/crustacean (90.6%); of which shrimp (30.3%), crab (15.2%), and lobster (3.0%) were the most common. Mild childhood asthma (69.7%), allergic rhinitis (6.3%), and eczema (6.1%) were the most common atopic conditions among individuals with shellfish/crustacean OAS. This pattern was similar for shellfish/crustacean anaphylaxis. Skin prick tests were most commonly positive for shrimp (OAS 87.1% vs. anaphylaxis 100%), crab (OAS 95.8% vs. 90.9%), and lobster (OAS 91.7% vs. 63.6%). OAS to shellfish/crustaceans was more common than anaphylaxis among this study population of young males referred for food allergy symptoms other than rash alone.

  17. Environmental links to interannual variability in shellfish toxicity in Cobscook Bay and eastern Maine, a strongly tidally mixed coastal region

    NASA Astrophysics Data System (ADS)

    Horecka, Hannah M.; Thomas, Andrew C.; Weatherbee, Ryan A.

    2014-05-01

    The Gulf of Maine experiences annual closures of shellfish harvesting due to the accumulation of toxins produced by dinoflagellates of the genus Alexandrium. Factors controlling the timing, location, and magnitude of these events in eastern Maine remain poorly understood. Previous work identified possible linkages between interannual variability of oceanographic variables and shellfish toxicity along the western Maine coastline but no such linkages were evident along the eastern Maine coast in the vicinity of Cobscook Bay, where strong tidal mixing tends to reduce seasonal variability in oceanographic properties. Using 21 years (1985-2005) of shellfish toxicity data, interannual variability in two metrics of annual toxicity, maximum magnitude and total annual toxicity, from stations in the Cobscook Bay region are examined for relationships to a suite of available environmental variables. Consistent with earlier work, no (or only weak) correlations were found between toxicity and oceanographic variables, even those very proximate to the stations such as local sea surface temperature. Similarly no correlations were evident between toxicity and air temperature, precipitation or relative humidity. The data suggest possible connections to local river discharge, but plausible mechanisms are not obvious. Correlations between toxicity and two variables indicative of local meteorological conditions, dew point and atmospheric pressure, both suggest a link between increased toxicity in these eastern Maine stations and weather conditions characterized by clearer skies/drier air (or less stormy/humid conditions). As no correlation of opposite sign was evident between toxicity and local precipitation, one plausible link is through light availability and its positive impact on phytoplankton production in this persistently foggy section of coast. These preliminary findings point to both the value of maintaining long-term shellfish toxicity sampling and a need for inclusion of weather variability in future modeling studies aimed at development of a more mechanistic understanding of factors controlling interannual differences in eastern Gulf of Maine shellfish toxicity.

  18. 9 CFR 71.18 - Individual identification of certain cattle 2 years of age or over for movement in interstate...

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... cattle 2 years of age or over for movement in interstate commerce. 71.18 Section 71.18 Animals and Animal... certain cattle 2 years of age or over for movement in interstate commerce. (a) No cattle 2 years of age or over, except steers and spayed heifers and cattle of any age which are being moved interstate during...

  19. 9 CFR 71.18 - Individual identification of certain cattle 2 years of age or over for movement in interstate...

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... cattle 2 years of age or over for movement in interstate commerce. 71.18 Section 71.18 Animals and Animal... certain cattle 2 years of age or over for movement in interstate commerce. (a) No cattle 2 years of age or over, except steers and spayed heifers and cattle of any age which are being moved interstate during...

  20. 9 CFR 71.18 - Individual identification of certain cattle 2 years of age or over for movement in interstate...

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... cattle 2 years of age or over for movement in interstate commerce. 71.18 Section 71.18 Animals and Animal... certain cattle 2 years of age or over for movement in interstate commerce. (a) No cattle 2 years of age or over, except steers and spayed heifers and cattle of any age which are being moved interstate during...

  1. 23 CFR Appendix C to Subpart A of... - Policy for the Signing and Numbering of Future Interstate Corridors Designated by Section 332 of...

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... Interstate Corridors Designated by Section 332 of the NHS Designation Act of 1995 or Designated Under 23 U.S... Interstate Corridors Designated by Section 332 of the NHS Designation Act of 1995 or Designated Under 23 U.S... signs along a federally designated future Interstate corridor only after the specific route location has...

  2. 23 CFR Appendix C to Subpart A of... - Policy for the Signing and Numbering of Future Interstate Corridors Designated by Section 332 of...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... Interstate Corridors Designated by Section 332 of the NHS Designation Act of 1995 or Designated Under 23 U.S... Interstate Corridors Designated by Section 332 of the NHS Designation Act of 1995 or Designated Under 23 U.S... signs along a federally designated future Interstate corridor only after the specific route location has...

  3. 23 CFR Appendix C to Subpart A of... - Policy for the Signing and Numbering of Future Interstate Corridors Designated by Section 332 of...

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... Interstate Corridors Designated by Section 332 of the NHS Designation Act of 1995 or Designated Under 23 U.S... Interstate Corridors Designated by Section 332 of the NHS Designation Act of 1995 or Designated Under 23 U.S... signs along a federally designated future Interstate corridor only after the specific route location has...

  4. An assessment of interstate safety investment properties in Washington state.

    DOT National Transportation Integrated Search

    2014-12-01

    The Washington State Department of Transportation (WSDOT) commissioned the current study, targeting the entire interstate : mainline network in Washington State, to provide strategic direction to multi-biennial investment interstate locations that of...

  5. 33 CFR 329.6 - Interstate or foreign commerce.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... United States. Note, however, that the mere presence of floating logs will not of itself make the river... the future, or at a past point in time. (b) Nature of commerce: interstate and intrastate. Interstate...

  6. 40 CFR 81.319 - Louisiana.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Parish Union Parish West Carroll Parish AQCR 022 Shreveport-Texarkana-Tyler Interstate Unclassifiable... Parish Union Parish West Carroll Parish AQCR 022 Shreveport-Texarkana-Tyler Interstate Unclassifiable...-Texarkana-Tyler Interstate Unclassifiable/Attainment Bienville Parish Bossier Parish Caddo Parish Claiborne...

  7. 40 CFR 81.319 - Louisiana.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Parish Union Parish West Carroll Parish AQCR 022 Shreveport-Texarkana-Tyler Interstate Unclassifiable... Parish West Carroll Parish AQCR 022 Shreveport-Texarkana-Tyler Interstate Unclassifiable/Attainment...-Texarkana-Tyler Interstate Unclassifiable/Attainment Bienville Parish Bossier Parish Caddo Parish Claiborne...

  8. 40 CFR 81.344 - Texas.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Attainment AQCR 022 Shreveport-Texarkana-Tyler Interstate Unclassifiable/Attainment Anderson County Bowie... Victoria Area: Victoria County Attainment AQCR 022 Shreveport-Texarkana-Tyler Interstate Unclassifiable... 022Shreveport-Texarkana-Tyler Interstate Unclassifiable/Attainment Anderson County Bowie County Camp County Cass...

  9. 40 CFR 81.319 - Louisiana.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Parish Union Parish West Carroll Parish AQCR 022 Shreveport-Texarkana-Tyler Interstate Unclassifiable... Parish West Carroll Parish AQCR 022 Shreveport-Texarkana-Tyler Interstate Unclassifiable/Attainment...-Texarkana-Tyler Interstate Unclassifiable/Attainment Bienville Parish Bossier Parish Caddo Parish Claiborne...

  10. 40 CFR 81.344 - Texas.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ...-Texarkana-Tyler Interstate Unclassifiable/Attainment Anderson County Bowie County Camp County Cass County...: Victoria County Attainment AQCR 022 Shreveport-Texarkana-Tyler Interstate Unclassifiable/Attainment...-Texarkana-Tyler Interstate Unclassifiable/Attainment Anderson County Bowie County Camp County Cass County...

  11. 40 CFR 81.344 - Texas.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ...-Texarkana-Tyler Interstate Unclassifiable/Attainment Anderson County Bowie County Camp County Cass County...: Victoria County Attainment AQCR 022 Shreveport-Texarkana-Tyler Interstate Unclassifiable/Attainment... 022Shreveport-Texarkana-Tyler Interstate Unclassifiable/Attainment Anderson County Bowie County Camp County Cass...

  12. 40 CFR 81.319 - Louisiana.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Parish Union Parish West Carroll Parish AQCR 022 Shreveport-Texarkana-Tyler Interstate Unclassifiable... Parish Union Parish West Carroll Parish AQCR 022 Shreveport-Texarkana-Tyler Interstate Unclassifiable...-Texarkana-Tyler Interstate Unclassifiable/Attainment Bienville Parish Bossier Parish Caddo Parish Claiborne...

  13. 40 CFR 81.344 - Texas.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ...-Texarkana-Tyler Interstate Unclassifiable/Attainment Anderson County Bowie County Camp County Cass County...: Victoria County Attainment AQCR 022 Shreveport-Texarkana-Tyler Interstate Unclassifiable/Attainment... 022Shreveport-Texarkana-Tyler Interstate Unclassifiable/Attainment Anderson County Bowie County Camp County Cass...

  14. Road Construction Safety Audit for Interstate Reconstruction. Volume 2.

    DOT National Transportation Integrated Search

    1998-10-01

    Traffic control alternatives associated with reconstruction projects on a rural interstate have been investigated in this research. Slab replacement projects, milling/resurfacing projects, and traffic controls in the vicinity of interstate ramps were...

  15. Road Construction Safety Audit for Interstate Reconstruction. Volume 1.

    DOT National Transportation Integrated Search

    1998-10-01

    Traffic control alternatives associated with reconstruction projects on a rural interstate have been investigated in this research. Slab replacement projects, milling/resurfacing projects, and traffic controls in the vicinity of interstate ramps were...

  16. The Contagion of Interstate Violence: Reminders of Historical Interstate (but Not Intrastate) Violence Increase Support for Future Violence Against Unrelated Third-Party States.

    PubMed

    Li, Mengyao; Leidner, Bernhard; Euh, Hyun; Choi, Hoon-Seok

    2016-08-01

    Five experiments investigated the war contagion phenomenon in the context of international relations, hypothesizing that reminders of past inter- (but not intra-) state war will increase support for future, unrelated interstate violence. After being reminded of the Korean War as an interstate rather than intrastate conflict, South Koreans showed stronger support for violent responses to new, unrelated interstate tensions (Study 1). Replicating this war contagion effect among Americans, we demonstrated that it was mediated by heightened perceived threat from, and negative images of, a fictitious country unrelated to the past war (Study 2), and moderated by national glorification (Study 3). Study 4, using another international conflict in the U.S. history, provided further conceptual replication. Finally, Study 5 included a baseline in addition to the inter- versus intrastate manipulation, yielding further support for the generalized effect of past interstate war reminders on preferences for aggressive approaches to new interstate tensions. © 2016 by the Society for Personality and Social Psychology, Inc.

  17. 49 CFR 1544.239 - Known shipper program.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... a TSA security program approved for transfer of cargo to an aircraft operator with a full program or...) Corrections and updates of this information upon learning of a change to the information specified in...

  18. 49 CFR 1544.239 - Known shipper program.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... a TSA security program approved for transfer of cargo to an aircraft operator with a full program or...) Corrections and updates of this information upon learning of a change to the information specified in...

  19. 49 CFR 1544.239 - Known shipper program.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... a TSA security program approved for transfer of cargo to an aircraft operator with a full program or...) Corrections and updates of this information upon learning of a change to the information specified in...

  20. 49 CFR 1544.239 - Known shipper program.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... a TSA security program approved for transfer of cargo to an aircraft operator with a full program or...) Corrections and updates of this information upon learning of a change to the information specified in...

  1. 49 CFR 1544.239 - Known shipper program.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... a TSA security program approved for transfer of cargo to an aircraft operator with a full program or...) Corrections and updates of this information upon learning of a change to the information specified in...

  2. DOE Office of Scientific and Technical Information (OSTI.GOV)

    Ferris, Y.M.

    The paper by A.S. Goldman, R.R. Picard, and J.P. Shipley of Los Alamos is critiqued, particularly with respect to the following: definition of safeguards, limits of accuracy, shipper/receiver differences, statistical terminology, material balance, and errors. (DLC)

  3. Recommendations and guidelines on shoreline development and hazards to navigation.

    DOT National Transportation Integrated Search

    2010-12-01

    This guidebook addresses recommendations regarding encroachment into the Gulf Coast : Intracoastal Waterway (GIWW). Encroachment of hazards to navigation creates operational : inefficiencies that impede commerce. The shippers who rely on the waterway...

  4. 9 CFR 93.704 - Import permit.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ...: (1) The name and address of the shipper in the region of origin of the hedgehog or tenrec intended... United States. (5) The mode of transportation. (6) The number, breed, species, and descriptions of the...

  5. 9 CFR 93.704 - Import permit.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ...: (1) The name and address of the shipper in the region of origin of the hedgehog or tenrec intended... United States. (5) The mode of transportation. (6) The number, breed, species, and descriptions of the...

  6. 9 CFR 93.704 - Import permit.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ...: (1) The name and address of the shipper in the region of origin of the hedgehog or tenrec intended... United States. (5) The mode of transportation. (6) The number, breed, species, and descriptions of the...

  7. 9 CFR 93.704 - Import permit.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ...: (1) The name and address of the shipper in the region of origin of the hedgehog or tenrec intended... United States. (5) The mode of transportation. (6) The number, breed, species, and descriptions of the...

  8. 9 CFR 93.704 - Import permit.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ...: (1) The name and address of the shipper in the region of origin of the hedgehog or tenrec intended... United States. (5) The mode of transportation. (6) The number, breed, species, and descriptions of the...

  9. 78 FR 18423 - Release of Waybill Data

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-26

    ... the Office of Economics. The waybill sample contains confidential railroad and shipper data; therefore... Board's Office of Economics within 14 calendar days of the date of this notice. The rules for release of...

  10. 77 FR 21835 - Release of Waybill Data

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-11

    ... the Office of Economics. The waybill sample contains confidential railroad and shipper data; therefore... Board's Office of Economics within 14 calendar days of the date of this notice. The rules for release of...

  11. 76 FR 81557 - Release of Waybill Data

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-28

    ... obtained from the Office of Economics. The waybill sample contains confidential railroad and shipper data... the Board's Office of Economics within 14 calendar days of the date of this notice. The rules for...

  12. 76 FR 1507 - Release of Waybill Data

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-10

    ... obtained from the Office of Economics. The waybill sample contains confidential railroad and shipper data... the Board's Office of Economics within 14 calendar days of the date of this notice. The rules for...

  13. 78 FR 10262 - Release of Waybill Data

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-13

    ... obtained from the Office of Economics. The waybill sample contains confidential railroad and shipper data... the Board's Office of Economics within 14 calendar days of the date of this notice. The rules for...

  14. 75 FR 64392 - Norfolk Southern Railway Company-Abandonment Exemption-in Polk County, IA; Iowa Interstate...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-19

    ..., IA; Iowa Interstate Railroad--Discontinuance of Service Exemption--in Polk County, IA Norfolk... Interstate Railroad, Ltd., 5900 6th Street, SW., Cedar Rapids, IA 52404. If the verified notice contains...

  15. 9 CFR 77.16 - Interstate movement from nonaccredited States and zones.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... PRODUCTS TUBERCULOSIS Cattle and Bison § 77.16 Interstate movement from nonaccredited States and zones... with or exposed to tuberculosis, may be moved interstate only if the cattle or bison are accompanied by...

  16. 9 CFR 77.31 - Interstate movement from nonaccredited States and zones.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... PRODUCTS TUBERCULOSIS Captive Cervids § 77.31 Interstate movement from nonaccredited States and zones... or exposed to tuberculosis may not be moved interstate only if they are accompanied by VS Form 1-27...

  17. 9 CFR 77.25 - Interstate movement from modified accredited advanced States and zones.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... ANIMAL PRODUCTS TUBERCULOSIS Captive Cervids § 77.25 Interstate movement from modified accredited..., and that are not known to be infected with or exposed to tuberculosis, may be moved interstate only...

  18. 9 CFR 77.8 - Interstate movement from accredited-free States and zones.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... PRODUCTS TUBERCULOSIS Cattle and Bison § 77.8 Interstate movement from accredited-free States and zones. Cattle or bison that originate in an accredited-free State or zone may be moved interstate without...

  19. 40 CFR 81.344 - Texas.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ...-Texarkana-Tyler Interstate Unclassifiable/Attainment Anderson County Bowie County Camp County Cass County... Victoria Area: Victoria County Attainment AQCR 022 Shreveport-Texarkana-Tyler Interstate Unclassifiable... 022Shreveport-Texarkana-Tyler Interstate Unclassifiable/Attainment Anderson County Bowie County Camp County Cass...

  20. 9 CFR 77.25 - Interstate movement from modified accredited advanced States and zones.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... ANIMAL PRODUCTS TUBERCULOSIS Captive Cervids § 77.25 Interstate movement from modified accredited..., and that are not known to be infected with or exposed to tuberculosis, may be moved interstate only...

  1. 9 CFR 77.16 - Interstate movement from nonaccredited States and zones.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... PRODUCTS TUBERCULOSIS Cattle and Bison § 77.16 Interstate movement from nonaccredited States and zones... with or exposed to tuberculosis, may be moved interstate only if the cattle or bison are accompanied by...

  2. 9 CFR 77.16 - Interstate movement from nonaccredited States and zones.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... PRODUCTS TUBERCULOSIS Cattle and Bison § 77.16 Interstate movement from nonaccredited States and zones... with or exposed to tuberculosis, may be moved interstate only if the cattle or bison are accompanied by...

  3. 9 CFR 77.31 - Interstate movement from nonaccredited States and zones.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... PRODUCTS TUBERCULOSIS Captive Cervids § 77.31 Interstate movement from nonaccredited States and zones... or exposed to tuberculosis may not be moved interstate only if they are accompanied by VS Form 1-27...

  4. 9 CFR 77.25 - Interstate movement from modified accredited advanced States and zones.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... ANIMAL PRODUCTS TUBERCULOSIS Captive Cervids § 77.25 Interstate movement from modified accredited..., and that are not known to be infected with or exposed to tuberculosis, may be moved interstate only in...

  5. 9 CFR 77.31 - Interstate movement from nonaccredited States and zones.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... PRODUCTS TUBERCULOSIS Captive Cervids § 77.31 Interstate movement from nonaccredited States and zones... or exposed to tuberculosis may not be moved interstate only if they are accompanied by VS Form 1-27...

  6. 9 CFR 77.31 - Interstate movement from nonaccredited States and zones.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... PRODUCTS TUBERCULOSIS Captive Cervids § 77.31 Interstate movement from nonaccredited States and zones... or exposed to tuberculosis may not be moved interstate only if they are accompanied by VS Form 1-27...

  7. 9 CFR 77.16 - Interstate movement from nonaccredited States and zones.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... PRODUCTS TUBERCULOSIS Cattle and Bison § 77.16 Interstate movement from nonaccredited States and zones... with or exposed to tuberculosis, may be moved interstate only if the cattle or bison are accompanied by...

  8. 9 CFR 77.16 - Interstate movement from nonaccredited States and zones.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... PRODUCTS TUBERCULOSIS Cattle and Bison § 77.16 Interstate movement from nonaccredited States and zones... with or exposed to tuberculosis, may be moved interstate only if the cattle or bison are accompanied by...

  9. 9 CFR 77.25 - Interstate movement from modified accredited advanced States and zones.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... ANIMAL PRODUCTS TUBERCULOSIS Captive Cervids § 77.25 Interstate movement from modified accredited..., and that are not known to be infected with or exposed to tuberculosis, may be moved interstate only...

  10. 9 CFR 77.31 - Interstate movement from nonaccredited States and zones.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... PRODUCTS TUBERCULOSIS Captive Cervids § 77.31 Interstate movement from nonaccredited States and zones... or exposed to tuberculosis may not be moved interstate only if they are accompanied by VS Form 1-27...

  11. 9 CFR 77.25 - Interstate movement from modified accredited advanced States and zones.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... ANIMAL PRODUCTS TUBERCULOSIS Captive Cervids § 77.25 Interstate movement from modified accredited..., and that are not known to be infected with or exposed to tuberculosis, may be moved interstate only...

  12. Complete genome sequence of the larval shellfish pathogen Vibrio Tubiashii type strain ATCC 19109

    USDA-ARS?s Scientific Manuscript database

    Vibrio tubiashii is a larval shellfish pathogen. Here we report the first closed genome sequence for this species (American Type Culture Collection type strain 19109), which has two chromosomes (3,294,490 and 1,766,582 bp), two megaplasmids (251,408 and 122,808 bp) and two plasmids (57,076 and 47,9...

  13. 36 CFR 242.25 - Subsistence taking of fish, wildlife, and shellfish: general regulations.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ..., mountain goat, moose, musk ox, Dall sheep, wolf, and wolverine. Bow means a longbow, recurve bow, or... (land) otter, red squirrel, flying squirrel, ground squirrel, marmot, wolf, or wolverine. Fyke net means...) Utilization of fish, wildlife, or shellfish. (1) You may not use wildlife as food for a dog or furbearer, or...

  14. 36 CFR 242.25 - Subsistence taking of fish, wildlife, and shellfish: general regulations.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ..., mountain goat, moose, musk ox, Dall sheep, wolf, and wolverine. Bow means a longbow, recurve bow, or... (land) otter, red squirrel, flying squirrel, ground squirrel, marmot, wolf, or wolverine. Fyke net means...) Utilization of fish, wildlife, or shellfish. (1) You may not use wildlife as food for a dog or furbearer, or...

  15. 36 CFR 242.25 - Subsistence taking of fish, wildlife, and shellfish: general regulations.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ..., mountain goat, moose, musk ox, Dall sheep, wolf, and wolverine. Bow means a longbow, recurve bow, or... (land) otter, red squirrel, flying squirrel, ground squirrel, marmot, wolf, or wolverine. Fyke net means...) Utilization of fish, wildlife, or shellfish. (1) You may not use wildlife as food for a dog or furbearer, or...

  16. 76 FR 15986 - Alpha Omega Technology, Inc.; Denial Without Prejudice of Food Additive Petition

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-22

    ... regulations be amended to provide for the safe use of a source of irradiation to treat shellfish and finfish....26) to provide for the safe use of a source of irradiation to treat finfish and shellfish. For any... pattern of typical spoilage organisms could be changed by irradiation, thus reducing perception of...

  17. Isolation of pandemic Vibrio parahaemolyticus from UK water and shellfish produce.

    PubMed

    Powell, Andy; Baker-Austin, Craig; Wagley, Sariqa; Bayley, Amanda; Hartnell, Rachel

    2013-05-01

    Vibrio parahaemolyticus is a Gram-negative, halophilic bacterium found commonly in temperate and warm estuarine waters worldwide. V. parahaemolyticus is considered an emerging bacterial pathogen in Europe and has been responsible for several recent seafood-associated outbreaks. During ad hoc testing of raw shellfish produce in May 2012, pandemic group (O3:K6) V. parahaemolyticus was isolated from Pacific oysters (Crassostrea gigas), harvested in Southern England. Follow-on testing of water and shellfish, encompassing a small number geographically diverse sites, also retrieved pandemic group isolates. These strains are amongst the most northerly pandemic strains described to date and represent the first instance of pandemic V. parahaemolyticus isolated in the UK, highlighting the expanding geographical distribution of these foodborne pathogens in the environment.

  18. 7 CFR 301.38 - Notice of quarantine; restrictions on interstate movement of regulated articles.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... QUARANTINE NOTICES Black Stem Rust § 301.38 Notice of quarantine; restrictions on interstate movement of... prevent the spread of black stem rust. No person shall move interstate any regulated article except in...

  19. 7 CFR 301.38 - Notice of quarantine; restrictions on interstate movement of regulated articles.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... QUARANTINE NOTICES Black Stem Rust § 301.38 Notice of quarantine; restrictions on interstate movement of... prevent the spread of black stem rust. No person shall move interstate any regulated article except in...

  20. 7 CFR 301.38 - Notice of quarantine; restrictions on interstate movement of regulated articles.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... QUARANTINE NOTICES Black Stem Rust § 301.38 Notice of quarantine; restrictions on interstate movement of... prevent the spread of black stem rust. No person shall move interstate any regulated article except in...

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