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Sample records for intrinsic optical signal

  1. Intrinsic optical signal imaging of retinal physiology: a review.

    PubMed

    Yao, Xincheng; Wang, Benquan

    2015-09-01

    Intrinsic optical signal (IOS) imaging promises to be a noninvasive method for high-resolution examination of retinal physiology, which can advance the study and diagnosis of eye diseases. While specialized optical instruments are desirable for functional IOS imaging of retinal physiology, in depth understanding of multiple IOS sources in the complex retinal neural network is essential for optimizing instrument designs. We provide a brief overview of IOS studies and relationships in rod outer segment suspensions, isolated retinas, and intact eyes. Recent developments of line-scan confocal and functional optical coherence tomography (OCT) instruments have allowed in vivo IOS mapping of photoreceptor physiology. Further improvements of the line-scan confocal and functional OCT systems may provide a feasible solution to pursue functional IOS mapping of human photoreceptors. Some interesting IOSs have already been detected in inner retinal layers, but better development of the IOS instruments and software algorithms is required to achieve optimal physiological assessment of inner retinal neurons.

  2. Intrinsic optical signal imaging of retinal physiology: a review

    NASA Astrophysics Data System (ADS)

    Yao, Xincheng; Wang, Benquan

    2015-09-01

    Intrinsic optical signal (IOS) imaging promises to be a noninvasive method for high-resolution examination of retinal physiology, which can advance the study and diagnosis of eye diseases. While specialized optical instruments are desirable for functional IOS imaging of retinal physiology, in depth understanding of multiple IOS sources in the complex retinal neural network is essential for optimizing instrument designs. We provide a brief overview of IOS studies and relationships in rod outer segment suspensions, isolated retinas, and intact eyes. Recent developments of line-scan confocal and functional optical coherence tomography (OCT) instruments have allowed in vivo IOS mapping of photoreceptor physiology. Further improvements of the line-scan confocal and functional OCT systems may provide a feasible solution to pursue functional IOS mapping of human photoreceptors. Some interesting IOSs have already been detected in inner retinal layers, but better development of the IOS instruments and software algorithms is required to achieve optimal physiological assessment of inner retinal neurons.

  3. Intrinsic optical signal imaging of retinal physiology: a review

    PubMed Central

    Yao, Xincheng; Wang, Benquan

    2015-01-01

    Abstract. Intrinsic optical signal (IOS) imaging promises to be a noninvasive method for high-resolution examination of retinal physiology, which can advance the study and diagnosis of eye diseases. While specialized optical instruments are desirable for functional IOS imaging of retinal physiology, in depth understanding of multiple IOS sources in the complex retinal neural network is essential for optimizing instrument designs. We provide a brief overview of IOS studies and relationships in rod outer segment suspensions, isolated retinas, and intact eyes. Recent developments of line-scan confocal and functional optical coherence tomography (OCT) instruments have allowed in vivo IOS mapping of photoreceptor physiology. Further improvements of the line-scan confocal and functional OCT systems may provide a feasible solution to pursue functional IOS mapping of human photoreceptors. Some interesting IOSs have already been detected in inner retinal layers, but better development of the IOS instruments and software algorithms is required to achieve optimal physiological assessment of inner retinal neurons. PMID:26405819

  4. In vivo intrinsic optical signal imaging of mouse retinas

    NASA Astrophysics Data System (ADS)

    Wang, Benquan; Yao, Xincheng

    2016-03-01

    Intrinsic optical signal (IOS) imaging is a promising noninvasive method for advanced study and diagnosis of eye diseases. Before pursuing clinical applications, more IOS studies employing animal models are necessary to establish the relationship between IOS distortions and eye diseases. Ample mouse models are available for investigating the relationship between IOS distortions and eye diseases. However, in vivo IOS imaging of mouse retinas is challenging due to the small ocular lens (compared to frog eyes) and inevitable eye movements. We report here in vivo IOS imaging of mouse retinas using a custom-designed functional OCT. The OCT system provided high resolution (3 μm) and high speed (up to 500 frames/s) imaging of mouse retinas. An animal holder equipped with a custom designed ear bar and bite bar was used to minimize eye movement due to breathing and heartbeats. Residual eye movement in OCT images was further compensated by accurate image registration. Dynamic OCT imaging revealed rapid IOSs from photoreceptor outer segments immediately (<10 ms) after the stimulation delivery, and unambiguous IOS changes were also observed from inner retinal layers with delayed time courses compared to that of photoreceptor IOSs.

  5. A comparison of signal processing techniques for Intrinsic Optical Signal imaging in mice.

    PubMed

    Turley, Jordan A; Nilsson, Michael; Walker, Frederick Rohan; Johnson, Sarah J

    2015-01-01

    Intrinsic Optical Signal imaging is a technique which allows the visualisation and mapping of activity related changes within the brain with excellent spatial and temporal resolution. We analysed a variety of signal and image processing techniques applied to real mouse imaging data. The results were compared in an attempt to overcome the unique issues faced when performing the technique on mice and improve the understanding of post processing options available.

  6. Hyperspectral optical tomography of intrinsic signals in the rat cortex.

    PubMed

    Konecky, Soren D; Wilson, Robert H; Hagen, Nathan; Mazhar, Amaan; Tkaczyk, Tomasz S; Frostig, Ron D; Tromberg, Bruce J

    2015-10-01

    We introduce a tomographic approach for three-dimensional imaging of evoked hemodynamic activity, using broadband illumination and diffuse optical tomography (DOT) image reconstruction. Changes in diffuse reflectance in the rat somatosensory cortex due to stimulation of a single whisker were imaged at a frame rate of 5 Hz using a hyperspectral image mapping spectrometer. In each frame, images in 38 wavelength bands from 484 to 652 nm were acquired simultaneously. For data analysis, we developed a hyperspectral DOT algorithm that used the Rytov approximation to quantify changes in tissue concentration of oxyhemoglobin ([Formula: see text]) and deoxyhemoglobin (ctHb) in three dimensions. Using this algorithm, the maximum changes in [Formula: see text] and ctHb were found to occur at [Formula: see text] and [Formula: see text] beneath the surface of the cortex, respectively. Rytov tomographic reconstructions revealed maximal spatially localized increases and decreases in [Formula: see text] and ctHb of [Formula: see text] and [Formula: see text], respectively, with these maximum changes occurring at [Formula: see text] poststimulus. The localized optical signals from the Rytov approximation were greater than those from modified Beer-Lambert, likely due in part to the inability of planar reflectance to account for partial volume effects. PMID:26835483

  7. Specific signal processing method for sound detected by an intrinsic optical fiber sensor

    NASA Astrophysics Data System (ADS)

    Arezki, M.; Meyrueis, P.; Javahiraly, N.

    2010-04-01

    Intrinsic optical fiber microphones with the design that we propose have a good sensitivity and dynamic allowing them to address many innovative applications. But their performances induce some defects as noise sensing that has to be separated to the wanted sound signal to obtain a clean usable sound signal. In this paper we introduce the design of an optical fiber sound sensing system and the bases of the adapted signal processing allowing to use all the advantages of this design with a low level of noise. The results are very promising.

  8. Intrinsic optical signals of brains in rats during loss of tissue viability: effect of brain temperature

    NASA Astrophysics Data System (ADS)

    Kawauchi, Satoko; Sato, Shunichi; Ooigawa, Hidetoshi; Nawashiro, Hiroshi; Kikuchi, Makoto

    2007-07-01

    Noninvasive, real-time monitoring of brain tissue viability is crucial for the patients with stroke, traumatic brain injury, etc. For this purpose, measurement of intrinsic optical signal (IOS) is attractive because it can provide direct information about the viability of brain tissue noninvasively. We performed simultaneous measurements of IOSs that are related to morphological characteristics, i.e., light scattering, and energy metabolism for rat brains during saline infusion as a model with temporal loss of brain tissue viability. The results showed that the scattering signal was steady in an initial phase but showed a drastic, triphasic change in a certain range of infusion time, during which the reduction of CuA in cytochrome c oxidase started and proceeded rapidly. The start time of triphasic scattering change was delayed for about 100 s by lowering brain temperature from 29°C to 24°C, demonstrating the optical detection of cerebroprotection effect by brain cooling. Electron microscopic observation showed morphological changes of dendrite and mitochondria in the cortical surface tissue after the triphasic scattering change, which was thought to be associated with the change in light scattering we observed. These findings suggest that the simultaneous measurement of the intrinsic optical signals related to morphological characteristics and energy metabolism is useful for monitoring tissue viability in brain.

  9. Long-term optical imaging of intrinsic signals in anesthetized and awake monkeys

    NASA Astrophysics Data System (ADS)

    Roe, Anna W.

    2007-04-01

    Some exciting new efforts to use intrinsic signal optical imaging methods for long-term studies in anesthetized and awake monkeys are reviewed. The development of such methodologies opens the door for studying behavioral states such as attention, motivation, memory, emotion, and other higher-order cognitive functions. Long-term imaging is also ideal for studying changes in the brain that accompany development, plasticity, and learning. Although intrinsic imaging lacks the temporal resolution offered by dyes, it is a high spatial resolution imaging method that does not require application of any external agents to the brain. The bulk of procedures described here have been developed in the monkey but can be applied to the study of surface structures in any in vivo preparation.

  10. Simultaneous imaging of intrinsic optical signals and cerebral vessel responses during cortical spreading depression in rats

    NASA Astrophysics Data System (ADS)

    Li, Pengcheng; Chen, Shangbin; Luo, Weihua; Luo, Qingming

    2003-12-01

    Cortical spreading depression (CSD) is an important disease model for migraine and cerebral ischemia. We investigated the spatio-temporal characteristics of the intrinsic optical signals (IOS) at 570 nm and the cerebral blood vessel responses during CSD simultaneously by optical reflectance imaging in vivo. The CSD were induced by pinprick in 10 α-chloralose/urethane anesthetized Sprague-Dawley rats. A four-phasic IOS response was observed at pial arteries and parenchymal sites in all experimental animals and an initial slight pial arteries dilation (21.5%+/-13.6%) and constriction (-4.2%+/-3.5%) precedes the dramatic dilation (69.2%+/-26.1%) of pial arterioles was recorded. Our experimental results show a high correlation (r = 0.89+/-0.025) between the IOS response and the diameter changes of the cerebral blood vessels during CSD in rats.

  11. Functional imaging of glucose-evoked rat islet activities using transient intrinsic optical signals

    NASA Astrophysics Data System (ADS)

    Yao, Xin-Cheng; Cui, Wan-Xing; Li, Yi-Chao; Zhang, Wei; Lu, Rong-Wen; Thompson, Anthony; Amthor, Franklin; Wang, Xu-Jing

    2012-05-01

    We demonstrate intrinsic optical signal (IOS) imaging of intact rat islet, which consists of many endocrine cells working together. A near-infrared digital microscope was employed for optical monitoring of islet activities evoked by glucose stimulation. Dynamic NIR images revealed transient IOS responses in the islet activated by low-dose (2.75 mM) and high-dose (5.5 mM) glucose stimuli. Comparative experiments and quantitative analysis indicated that both glucose metabolism and calcium/insulin dynamics might contribute to the observed IOS responses. Further investigation of the IOS imaging technology may provide a high resolution method for ex vivo functional examination of the islet, which is important for advanced study of diabetes associated islet dysfunctions and for improved quality control of donor islets for transplantation.

  12. Detection of low-amplitude in vivo intrinsic signals from an optical imager of retinal function

    NASA Astrophysics Data System (ADS)

    Barriga, Eduardo S.; T'so, Dan; Pattichis, Marios; Kwon, Young; Kardon, Randy; Abramoff, Michael; Soliz, Peter

    2006-02-01

    In the early stages of some retinal diseases, such as glaucoma, loss of retinal activity may be difficult to detect with today's clinical instruments. Many of today's instruments focus on detecting changes in anatomical structures, such as the nerve fiber layer. Our device, which is based on a modified fundus camera, seeks to detect changes in optical signals that reflect functional changes in the retina. The functional imager uses a patterned stimulus at wavelength of 535nm. An intrinsic functional signal is collected at a near infrared wavelength. Measured changes in reflectance in response to the visual stimulus are on the order of 0.1% to 1% of the total reflected intensity level, which makes the functional signal difficult to detect by standard methods because it is masked by other physiological signals and by imaging system noise. In this paper, we analyze the video sequences from a set of 60 experiments with different patterned stimuli from cats. Using a set of statistical techniques known as Independent Component Analysis (ICA), we estimate the signals present in the videos. Through controlled simulation experiments, we quantify the limits of signal strength in order to detect the physiological signal of interest. The results of the analysis show that, in principle, signal levels of 0.1% (-30dB) can be detected. The study found that in 86% of the animal experiments the patterned stimuli effects on the retina can be detected and extracted. The analysis of the different responses extracted from the videos can give an insight of the functional processes present during the stimulation of the retina.

  13. Potential sources of intrinsic optical signals imaged in live brain slices.

    PubMed

    Andrew, R D; Jarvis, C R; Obeidat, A S

    1999-06-01

    Changes in how light is absorbed or scattered in biological tissue are termed intrinsic optical signals (IOSs). Imaging IOSs in the submerged brain slice preparation provides insight into brain activity if it involves significant water movement between intracellular and extracellular compartments. This includes responses to osmotic imbalance, excitotoxic glutamate agonists, and oxygen/glucose deprivation, the latter leading to spreading depression. There are several misconceptions regarding these signals. (1) IOSs are not generated by glial swelling alone. Although neuronal and glia sources cannot yet be directly imaged, several lines of evidence indicate that neurons contribute significantly to the changes in light transmittance. (2) Excitotoxic swelling and osmotic swelling are physiologically different, as are their associated IOSs. Hyposmotic swelling involves no detectable neuronal depolarization of cortical pyramidal neurons, only the passive drawing in of water from a dilute medium across the cell membrane. In contrast excitotoxic swelling involves sustained membrane depolarization associated with inordinate amounts of Na+ and Cl- entry followed by water. IOSs demonstrate substantial damage in the latter case. (3) Osmotic perturbations do not induce volume regulatory mechanisms as measured by IOSs. The osmotic responses measured by IOSs in brain slices are passive, without the compensatory mechanisms that are assumed to be active on a scale suggested by studies of cultured brain cells under excessive osmotic stress. (4) Spreading depression (SD) can cause neuronal damage. Innocuous during migraine aura, SD induces acute neuronal damage in brain slices that are metabolically compromised by oxygen/glucose deprivation, as demonstrated by IOSs. Neighboring tissue where SD does not spread remains relatively healthy as judged by a minimal reduction in light transmittance. IOSs show that the metabolic stress of SD combined with the compromise of energy resources

  14. Elicitation interval dependent spatiotemporal evolution of cortical spreading depression waves revealed by optical intrinsic signal imaging

    NASA Astrophysics Data System (ADS)

    Chen, Shangbin; Gong, Hui; Zeng, Shaoqun; Luo, Qingming; Li, Pengcheng

    2007-02-01

    This study aimed to investigate the variation of propagation patterns of successive cortical spreading depression (CSD) waves induced by K + or pinprick in rat cortex. In the K + induction group, 18 Sprague-Dawley rats under Î+/--chloralose/urethane anesthesia were used to elicit CSD by 1 M KCl solution in the frontal cortex. Optical intrinsic signal imaging (OISI) at an isosbestic point of hemoglobin (550 nm) was applied to examine regional cerebral blood volume (CBV) changes in the parieto-occipital cortex. In 6 of the 18 rats, OISI was performed in conjunction with DC potential recording of the cortex. The results of this group were reported previously. In the pinprick group, 6 rats were used to induce CSD by pinprick with 8 min interval, and the other 6 rats were pricked with 4 min. CBV changes during CSD appeared as repetitive propagation of wave-like hyperemia at a speed of 3.7+/-0.4 mm/min, which was characterized by a significant negative peak (-14.3+/-3.2%) in the reflectance signal. Except for the first CSD wave, the following waves don't spread fully in the observed cortex all the time and they might abort in the medial area. Independent on the stimulation of pinprick or K+, a short interval of the current CSD to the last CSD no more than 4 min would induce the current CSD be partially propagated. For the first time, the data reveals the time-varying propagation patterns of CSD waves might be affected by the interval between CSD waves. The results suggest that the propagation patterns of a series of CSD waves are time-varying in different regions of rat cortex, and the variation is related to the interval between CSD waves.

  15. Separation of arteries and veins in the cerebral cortex using physiological oscillations by optical imaging of intrinsic signal

    NASA Astrophysics Data System (ADS)

    Hu, Dewen; Wang, Yucheng; Liu, Yadong; Li, Ming; Liu, Fayi

    2010-05-01

    An automated method is presented for artery-vein separation in cerebral cortical images recorded with optical imaging of the intrinsic signal. The vessel-type separation method is based on the fact that the spectral distribution of intrinsic physiological oscillations varies from arterial regions to venous regions. In arterial regions, the spectral power is higher in the heartbeat frequency (HF), whereas in venous regions, the spectral power is higher in the respiration frequency (RF). The separation method was begun by extracting the vascular network and its centerline. Then the spectra of the optical intrinsic signals were estimated by the multitaper method. A standard F-test was performed on each discrete frequency point to test the statistical significance at the given level. Four periodic physiological oscillations were examined: HF, RF, and two other eigenfrequencies termed F1 and F2. The separation of arteries and veins was implemented with the fuzzy c-means clustering method and the region-growing approach by utilizing the spectral amplitudes and power-ratio values of the four eigenfrequencies on the vasculature. Subsequently, independent spectral distributions in the arteries, veins, and capillary bed were estimated for comparison, which showed that the spectral distributions of the intrinsic signals were very distinct between the arterial and venous regions.

  16. Intrinsic optical signal imaging of glucose-stimulated physiological responses in the insulin secreting INS-1 β-cell line

    NASA Astrophysics Data System (ADS)

    Li, Yi-Chao; Cui, Wan-Xing; Wang, Xu-Jing; Amthor, Franklin; Yao, Xin-Cheng

    2011-03-01

    Intrinsic optical signal (IOS) imaging has been established for noninvasive monitoring of stimulus-evoked physiological responses in the retina and other neural tissues. Recently, we extended the IOS imaging technology for functional evaluation of insulin secreting INS-1 cells. INS-1 cells provide a popular model for investigating β-cell dysfunction and diabetes. Our experiments indicate that IOS imaging allows simultaneous monitoring of glucose-stimulated physiological responses in multiple cells with high spatial (sub-cellular) and temporal (sub-second) resolution. Rapid image sequences reveal transient optical responses that have time courses comparable to glucose-evoked β-cell electrical activities.

  17. Plastic Change along the Intact Crossed Pathway in Acute Phase of Cerebral Ischemia Revealed by Optical Intrinsic Signal Imaging

    PubMed Central

    Guo, Xiaoli; He, Yongzhi; Lu, Hongyang; Li, Yao; Su, Xin; Jiang, Ying; Tong, Shanbao

    2016-01-01

    The intact crossed pathway via which the contralesional hemisphere responds to the ipsilesional somatosensory input has shown to be affected by unilateral stroke. The aim of this study was to investigate the plasticity of the intact crossed pathway in response to different intensities of stimulation in a rodent photothrombotic stroke model. Using optical intrinsic signal imaging, an overall increase of the contralesional cortical response was observed in the acute phase (≤48 hours) after stroke. In particular, the contralesional hyperactivation is more prominent under weak stimulations, while a strong stimulation would even elicit a depressed response. The results suggest a distinct stimulation-response pattern along the intact crossed pathway after stroke. We speculate that the contralesional hyperactivation under weak stimulations was due to the reorganization for compensatory response to the weak ipsilateral somatosensory input. PMID:27144032

  18. In-vivo imaging of stimulus-evoked intrinsic optical signals correlated with retinal activation in anesthetized frog

    NASA Astrophysics Data System (ADS)

    Yao, Xin-Cheng; Zhang, Qiu-Xiang; Li, Yang-Guo

    2011-09-01

    Intrinsic optical signal imaging (IOS) promises a noninvasive method for high resolution examination of retinal function. Using freshly isolated animal retinas, we have conducted a series of experiments to test fast IOSs which have time courses comparable to electrophysiological kinetics. In this article, we demonstrate the feasibility of in vivo imaging of fast IOSs correlated with retinal activation in anesthetized frog (Rana Pipiens). A rapid (68,000 lines/s) line-scan confocal ophthalmoscope was constructed to achieve high-speed (200 frames/s) near infared (NIR) recording of fast IOSs. By rejecting out-of-focus background light, the line-scan confocal imager provided enough resolution to differentiate individual photoreceptors in vivo. With visible light stimulation, NIR confocal images disclosed transient IOSs with time courses comparable to retinal ERG kinetics. High-resolution IOS images revealed both positive (increasing) and negative (decreasing) light responses, with sub-cellular complexity, in the activated retina.

  19. Imaging nanomaterials in vitro and in vivo by exploring their intrinsic nonlinear optical signals

    NASA Astrophysics Data System (ADS)

    Tong, Ling

    The extension of nanotechnology to biomedical system creates a new and fast developing field, nanomedicine. A wide range of nanoparticles has been developed as imaging agents or drug carriers. However, the translation of nanomedicines to a clinical setting has been slowed down due to a limited fundamental understanding of the nano-bio interaction. My thesis work describes the efforts in imaging the behavior of nanomaterials in live cells and animals by exploring the nonlinear optical properties. The first part of my thesis focuses on study of metallic and semiconducting nanoparticles in biological environment using their nonlinear optical signals. In chapter 2, systemic circulation of PEGylated gold nanorods (GNRs) is visualized by intravital two-photon luminescence (TPL) imaging. A biphasic clearance is demonstrated with branched PEG showing longer circulation. Following clearance, cellular biodistribution of GNRs in organs is mapped by TPL imaging. GNRs accumulate in macrophages in liver and spleen (Langmuir, 2009, 25, 12454-12459). In chapter 3, a bright three-photon luminescence is discovered from Au-Ag alloyed nanostructure by excitation with a femtosecond laser at 1290 nm, which enables bio-imaging with negligible photothermal toxicity and tissue autofluorescence (Angew Chemie, 2010, 49, 3485-3488, inside cover story). In chapter 4, a new contrast is invented for label-free, real-time imaging of single-walled carbon nanotubes (SWNTs) by pump-probe microscopy. At pump/probe wavelength of 707 and 885 nm, semiconducting and metallic SWNTs (S-SWNTs and M-SWNTs) exhibit intense stimulated emission and absorption signals, which allow us to monitor the intracellular trafficking, distribution in tissues, and systemic circulation in vivo with single-nanotube sensitivity and sub-micron resolution. The second part presents label-free imaging of nanomedicines in live cells by coherent anti-Stokes Raman scattering (CARS) and stimulated Raman scattering (SRS) microscopy

  20. Changes in intrinsic optical signals during loss of tissue viability of brains in rats: effect of brain temperature

    NASA Astrophysics Data System (ADS)

    Kawauchi, Satoko; Sato, Shunichi; Ooigawa, Hidetoshi; Nawashiro, Hiroshi; Kikuchi, Makoto

    2007-02-01

    To investigate the temperature dependence of intrinsic optical signals (IOSs) relating to brain tissue viability, we performed simultaneous measurement of light absorption due to the redox states of cytochrome c oxidase and light scattering, which reflects morphological characteristics of cells in tissue, for rat brains after blood removal by saline infusion at different infusion temperatures. To determine IOSs at each temperature, we first examined an isosbestic wavelength of the redox states of cytochtome c oxidase for each rat based on multiwavelength diffuse reflectance measurement. We then measured diffuse reflectance intensity at the isosbestic wavelength as a scattering signal, while diffuse reflectance intensity at 800 nm was detected to monitor the reduction of CuA in cytochrome c oxidase. At all temperatures, the scattering signal was steady in an early phase but showed a drastic, triphasic change in a certain time range of infusion; during this scattering change, the reduction of CuA started and proceeded rapidly. The start time of triphasic scattering change as well as the start time of the reduction of CuA was extended for more than 2 min by lowering infusion temperature from 30 to 23°C and we found that there was a linear correlation between these two start times. These results suggest that tissue metabolic activity can be maintained for longer time by keeping the brain at lower temperature, and triphasic scattering change can be used as an optical signal indicating the reduction of CuA in cytochrome c oxidase, and hence loss of tissue viability for brain.

  1. Correlation between the durations of refractory period and intrinsic optical signal of retinal spreading depression during temperature variations.

    PubMed

    Weimer, Marc S; Hanke, Wolfgang

    2005-02-01

    Spreading depression (SD) is a neurophysiological phenomenon which occurs in the grey substance of the central nervous system. SD is characterised by a wave-like spread of depressed neuronal activity, by large ion shifts between intra- and extracellular space, by cellular depolarization, and by altered optical properties of the tissue giving rise to an intrinsic optical signal (IOS). In the shadow of SD further waves are difficult to trigger and such waves spread at lower velocity than usual. In this paper we examine the temperature dependence of the duration of this recovery (refractory) period and the temperature dependence of the duration of the IOS in the chicken retina. It is shown that these SD accompanying events are strongly dependent on temperature and that they are likely to depend on the metabolic rate in the tissue. The observed correlation of the duration of the IOS with the duration of the refractory period suggests that the IOS is a good indicator for the duration of the tissue recovery. Such a correlation would be of great value to the experimentalist who must know about the duration of the refractory period: while the latter is laborious to determine, recording the IOS is convenient.

  2. High-Resolution fMRI Maps of Cortical Activation in Nonhuman Primates: Correlation with Intrinsic Signal Optical Images

    PubMed Central

    Roe, Anna W.; Chen, Li Min

    2009-01-01

    One of the most widely used functional brain mapping tools is blood oxygen level–dependent (BOLD) functional magnetic resonance imaging (fMRI). This method has contributed to new understandings of the functional roles of different areas in the human brain. However, its ability to map cerebral cortex at high spatial (submillimeter) resolution is still unknown. Other methods such as single- and multiunit electrophysiology and intrinsic signal optical imaging have revealed submillimeter resolution of sensory topography and cortical columnar activations. However, they are limited either by spatial scale (electrophysiology characterizes only local groups of neurons) or by the inability to monitor deep structures in the brain (i.e., cortical regions buried in sulci or subcortical structures). A method that could monitor all regions of the brain at high spatial resolution would be ideal. This capacity would open the doors to investigating, for example, how networks of cerebral cortical columns relate to or produce behavior. In this article we demonstrate that, without benefit of contrast agents, at a magnetic field strength of 9.4 tesla, BOLD fMRI can reveal millimeter-sized topographic maps of digit representation in the somatosensory cortex of the anesthetized squirrel monkey. Furthermore, by mapping the “funneling illusion,” it is possible to detect even submillimeter shifts in activation in the cortex. Our data suggest that at high magnetic field strength, the positive BOLD signal can be used to reveal high spatial resolution maps of brain activity, a finding that weakens previous notions about the ultimate spatial specificity of the positive BOLD signal. PMID:18172338

  3. Control of anoxic depolarization in rat brain by near-infrared laser irradiation and its monitoring by intrinsic optical signal imaging

    NASA Astrophysics Data System (ADS)

    Kawauchi, Satoko; Sato, Shunichi; Uozumi, Yoichi; Nawashiro, Hiroshi; Ishihara, Miya; Ashida, Hiroshi

    2012-03-01

    In brain anoxia or ischemia, spreading depolarization is a key event that deterimines brain tissue survival. After onset of anoxia/ischemia, impairment of energy metabolism causes anoxic/ischemic depolarization (AD), which considerably consumes energy, leading to acute neuronal death in the brain. Our previous intrinsic-optical-signal imaging for the rat brains showed that about 2 min after starting hypoxia, AD-related light-scattering waves were focally generated in the bilateral outermost regions in the cortex and spread toward the midline, indicating that AD can be monitored by lightscattering signal. The behaviors of the scattering waves were found to be correlated with the survival of the rats. In the present study, we used the scattering signal-based monitoring method for AD and examined whether near-infrared laser irradiation can control AD in the rat brains. The left hemisphere was irradiated with 808-nm laser transcranially at 7.5 mW/cm2 before (30 min) and during hypoxia. The onset time of the scattering wave (AD) was significantly delayed in the irradiated hemisphere when compared with that in the non-irradiated hemisphere (3.4 s, n=8). The area of AD spreading in the irradiated hemisphere was significantly smaller than that in the non-irradiated hemisphere (27-90% reduction at 10-50 s after AD onset). These results suggest that near-infrared light can delay and reduce anoxic depolarization in the brain, which is probably due to increase in the cerebral ATP by near-infrared laser irradiation.

  4. Dynamic differential imaging of intrinsic optical responses in the retina

    NASA Astrophysics Data System (ADS)

    Yao, Xin-Cheng; Li, Yang-Guo; Liu, Lei; Amthor, Frank

    2010-02-01

    High resolution monitoring of stimulus-evoked retinal neural activities is important for understanding retinal neural mechanisms, and for diagnosis of retinal disease and evaluation of treatment. Fast intrinsic optical signals (IOSs), which have the time courses comparable to retinal electrophysiological responses, hold the promise for high resolution imaging of retinal neural activities. However, application of fast IOS imaging has been hindered by contamination of slow, high magnitude, optical responses associated with transient hemodynamic and metabolic changes. We recently demonstrated the feasibility of separating fast IOSs from slow optical responses by combined dynamic differential imaging and high frequency flicker stimulation.

  5. Optical signal processing

    NASA Technical Reports Server (NTRS)

    Casasent, D.

    1978-01-01

    The article discusses several optical configurations used for signal processing. Electronic-to-optical transducers are outlined, noting fixed window transducers and moving window acousto-optic transducers. Folded spectrum techniques are considered, with reference to wideband RF signal analysis, fetal electroencephalogram analysis, engine vibration analysis, signal buried in noise, and spatial filtering. Various methods for radar signal processing are described, such as phased-array antennas, the optical processing of phased-array data, pulsed Doppler and FM radar systems, a multichannel one-dimensional optical correlator, correlations with long coded waveforms, and Doppler signal processing. Means for noncoherent optical signal processing are noted, including an optical correlator for speech recognition and a noncoherent optical correlator.

  6. Optical signal processing: Musical score for optical signals

    NASA Astrophysics Data System (ADS)

    Testorf, Markus

    2012-07-01

    Phase-space optics is an indispensable tool for optical imaging and sensing. New optical hardware for light-field photography and pupil engineering for imaging with extended depth of field promote the use of phase-space representations as the primary object of optical signal processing.

  7. Time domain optical susceptibility of intrinsic GaAs

    NASA Astrophysics Data System (ADS)

    Thomas, M. E.; Miragliotta, J. A.; Joseph, R. I.

    2002-06-01

    Intrinsic GaAs optical constant values are well known as functions of frequency (10 000-65 000 cm-1 or 1.24-8.06 eV) and temperature (22-754 K). Room-temperature far-infrared optical constant data also exist as a function of frequency, and are representable by a classical oscillator model. In this article, the frequency-domain, temperature-dependent intrinsic dielectric function of GaAs has been Fourier transformed to obtain an analytical, closed-form representation of the time-domain susceptibility. Results from these expressions are consistent with the temporal characteristics of electronic transitions impeded by elastic scattering, which are in the femtosecond regime. The closed form nature of these expressions makes them well suited for finite difference time domain simulations of waveguides, optoelectronic devices, and microwave devices.

  8. Histone deacetylase 4 possesses intrinsic nuclear import and export signals.

    PubMed

    Wang, A H; Yang, X J

    2001-09-01

    Nucleocytoplasmic trafficking of histone deacetylase 4 (HDAC4) plays an important role in regulating its function, and binding of 14-3-3 proteins is necessary for its cytoplasmic retention. Here, we report the identification of nuclear import and export sequences of HDAC4. While its N-terminal 118 residues modulate the nuclear localization, residues 244 to 279 constitute an authentic, strong nuclear localization signal. Mutational analysis of this signal revealed that three arginine-lysine clusters are necessary for its nuclear import activity. As for nuclear export, leucine-rich sequences located in the middle part of HDAC4 do not function as nuclear export signals. By contrast, a hydrophobic motif (MXXLXVXV) located at the C-terminal end serves as a nuclear export signal that is necessary for cytoplasmic retention of HDAC4. This motif is required for CRM1-mediated nuclear export of HDAC4. Furthermore, binding of 14-3-3 proteins promotes cytoplasmic localization of HDAC4 by both inhibiting its nuclear import and stimulating its nuclear export. Unlike wild-type HDAC4, a point mutant with abrogated MEF2-binding ability remains cytoplasmic upon exogenous expression of MEF2C, supporting the notion that direct MEF2 binding targets HDAC4 to the nucleus. Therefore, HDAC4 possesses intrinsic nuclear import and export signals for its dynamic nucleocytoplasmic shuttling, and association with 14-3-3 and MEF2 proteins affects such shuttling and thus directs HDAC4 to the cytoplasm and the nucleus, respectively.

  9. Molecular signaling involving intrinsically disordered proteins in prostate cancer

    PubMed Central

    Russo, Anna; Manna, Sara La; Novellino, Ettore; Malfitano, Anna Maria; Marasco, Daniela

    2016-01-01

    Investigations on cellular protein interaction networks (PINs) reveal that proteins that constitute hubs in a PIN are notably enriched in Intrinsically Disordered Proteins (IDPs) compared to proteins that constitute edges, highlighting the role of IDPs in signaling pathways. Most IDPs rapidly undergo disorder-to-order transitions upon binding to their biological targets to perform their function. Conformational dynamics enables IDPs to be versatile and to interact with a broad range of interactors under normal physiological conditions where their expression is tightly modulated. IDPs are involved in many cellular processes such as cellular signaling, transcriptional regulation, and splicing; thus, their high-specificity/low-affinity interactions play crucial roles in many human diseases including cancer. Prostate cancer (PCa) is one of the leading causes of cancer-related mortality in men worldwide. Therefore, identifying molecular mechanisms of the oncogenic signaling pathways that are involved in prostate carcinogenesis is crucial. In this review, we focus on the aspects of cellular pathways leading to PCa in which IDPs exert a primary role. PMID:27212129

  10. Intrinsic Fabry-Perot optical fiber sensors and their multiplexing

    DOEpatents

    Wang, Anbo

    2007-12-11

    An intrinsic Fabry-Perot optical sensor includes a thin film sandwiched between two fiber ends. When light is launched into the fiber, two reflections are generated at the two fiber/thin film interfaces due to a difference in refractive indices between the fibers and the film, giving rise to the sensor output. In another embodiment, a portion of the cladding of a fiber is removed, creating two parallel surfaces. Part of the evanescent fields of light propagating in the fiber is reflected at each of the surfaces, giving rise to the sensor output. In a third embodiment, the refractive index of a small portion of a fiber is changed through exposure to a laser beam or other radiation. Interference between reflections at the ends of the small portion give rise to the sensor output. Multiple sensors along a single fiber are multiplexed using an optical time domain reflectometry method.

  11. Intrinsic optical bistability in a strongly driven Rydberg ensemble

    NASA Astrophysics Data System (ADS)

    de Melo, Natalia R.; Wade, Christopher G.; Šibalić, Nikola; Kondo, Jorge M.; Adams, Charles S.; Weatherill, Kevin J.

    2016-06-01

    We observe and characterize intrinsic optical bistability in a dilute Rydberg vapor. The bistability is characterized by sharp jumps between states of low and high Rydberg occupancy with jump-up and -down positions displaying hysteresis depending on the direction in which the control parameter is changed. We find that the shift in frequency of the jump point scales with the fourth power of the principal quantum number. Also, the width of the hysteresis window increases with increasing principal quantum number, before reaching a peak and then closing again. The experimental results are consistent with predictions from a simple theoretical model based on semiclassical Maxwell-Bloch equations including the effects of interaction-induced broadening and level shifts. These results provide insight into the dynamics of driven dissipative systems.

  12. Intrinsic Localized Modes in Optical Photonic Lattices and Arrays

    NASA Astrophysics Data System (ADS)

    Christodoulides, Demetrios

    -locking and pulse compression. A strong signature of discrete X-wave formation was also demonstrated in such structures. In the last few years, Anderson localization was unequivocally observed in array systems where the transition from ballistic transport to diffusive, and the cross-over to Anderson localization was studied as a function of disorder and nonlinearity. In recent studies synthetic lattices exhibiting parity-time (PT) symmetry were also considered. The interplay of gain and loss in this latter family of structures leads to counterintuitive characteristics and behavior such as non-reciprocal propagation and power oscillations. The realization of discrete array systems at su-bwavelenth scales is another important direction that is nowadays intensively pursued. References 1. D. N. Christodoulides, F. Lederer, and Y. Silberberg, Nature 424, 817- 823 (2003). 2. F. Lederer, G. I. Stegeman, D. N. Christodoulides, G. Assanto, M. Segev and Y. Silberberg, Phys. Reports 463, 1-126 (2008). 3. M Wimmer, A Regensburger, MA Miri, C. Bersch, D.N Christodoulides, and U. Peschel, ''Observation of optical solitons in PT-symmetric lattices'' Nature Communications 6, 7782 (2015). Intrinsic Localized Modes in Optical Photonic Lattices and Arrays.

  13. Sensory-Evoked Intrinsic Imaging Signals in the Olfactory Bulb Are Independent of Neurovascular Coupling

    PubMed Central

    Vincis, Roberto; Lagier, Samuel; Van De Ville, Dimitri; Rodriguez, Ivan; Carleton, Alan

    2016-01-01

    Summary Functional brain-imaging techniques used in humans and animals, such as functional MRI and intrinsic optical signal (IOS) imaging, are thought to largely rely on neurovascular coupling and hemodynamic responses. Here, taking advantage of the well-described micro-architecture of the mouse olfactory bulb, we dissected the nature of odor-evoked IOSs. Using in vivo pharmacology in transgenic mouse lines reporting activity in different cell types, we show that parenchymal IOSs are largely independent of neurotransmitter release and neurovascular coupling. Furthermore, our results suggest that odor-evoked parenchymal IOSs originate from changes in light scattering of olfactory sensory neuron axons, mostly due to water movement following action potential propagation. Our study sheds light on a direct correlate of neuronal activity, which may be used for large-scale functional brain imaging. PMID:26146075

  14. Intrinsic chemosensory signal recorded from the human nasal mucosa in patients with smell loss.

    PubMed

    Ishimaru, Tadashi; Krone, Franziska; Scheibe, Mandy; Gudziol, Volker; Negoias, Simona; Hummel, Thomas

    2013-03-01

    Physiological investigation of olfactory receptor function in hyposmic or anosmic patients is rare. Pioneers examined the electro-olfactogram in patients with olfactory disturbance. Although the electro-olfactogram is an established method to record olfactory responses from human olfactory epithelium, the response is only measured at specific sites of the olfactory mucosa. In contrast to that the response of the olfactory epithelium to chemosensory stimuli can be studied in a specific nasal area by means of intrinsic optical signal recording. Five functionally anosmic patients were included in the present study. In all patients, responses could be obtained following trigeminal stimulation with CO2. In some patients, responses could be obtained after olfactory stimulation with H2S and PEA. The present data show that in the studied patients trigeminal function seems to be preserved, while it appears that in some patients olfactory function is preserved to a certain degree.

  15. Optical investigations of physiology: a study of intrinsic and extrinsic biomedical contrast.

    PubMed Central

    Chance, B; Luo, Q; Nioka, S; Alsop, D C; Detre, J A

    1997-01-01

    The utility and performance of optical studies of tissue depends upon the contrast and the changes of contrast in health and disease and in functional activity. The contrast is determined both by the optical properties of extrinsic chromophores and scatterers but especially upon the changes evoked by physiological activity and pathological states. Here, we have focused upon absorption changes of the intrinsic probe, blood absorbance changes due to cortical hypoxia and to haematomas, giving, for particular conditions, absorbance changes of 0.15 and over 0.4 delta OD, respectively. Functional activity may give changes of blood volume of over 0.05 delta OD with some variability due to individual responses that is best expressed as histogram displays of the distribution of response among a significant population. Responses have been observed in prefrontal parietal and occipital functions (242 tests). Extrinsic probes afford signals dependent upon the dose tolerance of the subject and can readily equal or exceed the blood volume and oxygenation signals, and currently afford vascular volume and flow indications. However, contrast agents for the functional activity of cellular function are ultimately to be expected. Finally, light-scattering changes afford osmolyte-related responses and are here shown to indicate a larger signal attributed to cortical depolarization and K+ release in hypoxia/ischaemia. Thus, the optical method affords imaging of manifold contrasts that greatly enhance its specificity and sensitivity for diagnostic procedures. PMID:9232859

  16. SEMICONDUCTOR DEVICES Intrinsic stability of an HBT based on a small signal equivalent circuit model

    NASA Astrophysics Data System (ADS)

    Yanhu, Chen; Huajun, Shen; Xinyu, Liu; Huijun, Li; Hui, Xu; Ling, Li

    2010-12-01

    Intrinsic stability of the heterojunction bipolar transistor (HBT) was analyzed and discussed based on a small signal equivalent circuit model. The stability factor of the HBT device was derived based on a compact T-type small signal equivalent circuit model of the HBT. The effect of the mainly small signal model parameters of the HBT on the stability of the HBT was thoroughly examined. The discipline of parameter optimum to improve the intrinsic stability of the HBT was achieved. The theoretic analysis results of the stability were also used to explain the experimental results of the stability of the HBT and they were verified by the experimental results.

  17. Intrinsic noise induced resonance in presence of sub-threshold signal in Brusselator

    NASA Astrophysics Data System (ADS)

    Dey, Supravat; Das, Dibyendu; Parmananda, P.

    2011-09-01

    In a system of non-linear chemical reactions called the Brusselator, we show that intrinsic noise can be regulated to drive it to exhibit resonance in the presence of a sub-threshold signal. The phenomena of periodic stochastic resonance and aperiodic stochastic resonance, hitherto studied mostly with extrinsic noise, is demonstrated here to occur with inherent systemic noise using exact stochastic simulation algorithm due to Gillespie. The role of intrinsic noise in a couple of other phenomena is also discussed.

  18. Quantitative biomarkers of colonic dysplasia based on intrinsic second-harmonic generation signal.

    PubMed

    Zhuo, Shuangmu; Zhu, Xiaoqin; Wu, Guizhu; Chen, Jianxin; Xie, Shusen

    2011-12-01

    Most colorectal cancers arise from dysplastic lesions, such as adenomatous polyps, and these lesions are difficult to be detected by the current endoscopic screening approaches. Here, we present the use of an intrinsic second-harmonic generation (SHG) signal as a novel means to differentiate between normal and dysplastic human colonic tissues. We find that the SHG signal can quantitatively identify collagen change associated with colonic dysplasia that is indiscernible by conventional pathologic techniques. By comparing normal with dysplastic mucosa, there were significant differences in collagen density and collagen fiber direction, providing substantial potential to become quantitative intrinsic biomarkers for in vivo clinical diagnosis of colonic dysplasia.

  19. Effect of reference spectra in spectral fitting to discriminate enzyme-activatable photoacoustic probe from intrinsic optical absorbers

    NASA Astrophysics Data System (ADS)

    Hirasawa, Takeshi; Okawa, Shinpei; Iwatate, Ryu J.; Kamiya, Mako; Urano, Yasuteru; Ishihara, Miya

    2016-03-01

    Multispectral photoacoustic (MS-PA) imaging has been researched to image molecular probes in the presence of strong background signals produced from intrinsic optical absorbers. Spectral fitting method (SFM) discriminates probe signals from background signals by fitting the PA spectra that are calculated from MS-PA images to reference spectra of the probe and background, respectively. Because hemoglobin is a dominant optical absorber in visible to near-infrared wavelength range, absorption spectra of hemoglobin have been widely used as reference background spectra. However, the spectra of background signals produced from heterogeneous biological tissue differ from the reference background spectra due to presence of other intrinsic optical absorbers and effect of optical scattering. Due to the difference, the background signals partly remain in the probe images. To image the probe injected in subcutaneous tumors of mice clearly, we added the melanosome absorption spectrum to the reference background spectra because skin contains nonnegligible concentration of melanosome and the spectrum is very similar to the scattering spectrum of biological tissue. The probe injected in the subcutaneous tumor of mice was an enzyme-activatable probe which show their original colors only in the presence of γ-glutamyltranspeptidase, an enzyme associated with cancer. The probes have been successfully used for rapid fluorescence imaging of cancer. As a result of MS-PA imaging, by considering the melanosome absorption spectrum, the background signals were successfully suppressed and then clearer probe image was obtained. Our MS-PA imaging method afforded successful imaging of tumors in mice injected with activatable PA probes.

  20. Intrinsic stability of quantum cascade lasers against optical feedback.

    PubMed

    Mezzapesa, F P; Columbo, L L; Brambilla, M; Dabbicco, M; Borri, S; Vitiello, M S; Beere, H E; Ritchie, D A; Scamarcio, G

    2013-06-01

    We study the time dependence of the optical power emitted by terahertz and mid-IR quantum cascade lasers in presence of optical reinjection and demonstrate unprecedented continuous wave (CW) emission stability for strong feedback. We show that the absence of coherence collapse or other CW instabilities typical of diode lasers is inherently associated with the high value of the photon to carrier lifetime ratio and the negligible linewidth enhancement factor of quantum cascade lasers.

  1. Optically isolated signal coupler with linear response

    DOEpatents

    Kronberg, James W.

    1994-01-01

    An optocoupler for isolating electrical signals that translates an electrical input signal linearly to an electrical output signal. The optocoupler comprises a light emitter, a light receiver, and a light transmitting medium. The light emitter, preferably a blue, silicon carbide LED, is of the type that provides linear, electro-optical conversion of electrical signals within a narrow wavelength range. Correspondingly, the light receiver, which converts light signals to electrical signals and is preferably a cadmium sulfide photoconductor, is linearly responsive to light signals within substantially the same wavelength range as the blue LED.

  2. Intrinsic noise induced resonance in presence of sub-threshold signal in Brusselator.

    PubMed

    Dey, Supravat; Das, Dibyendu; Parmananda, P

    2011-09-01

    In a system of non-linear chemical reactions called the Brusselator, we show that intrinsic noise can be regulated to drive it to exhibit resonance in the presence of a sub-threshold signal. The phenomena of periodic stochastic resonance and aperiodic stochastic resonance, hitherto studied mostly with extrinsic noise, is demonstrated here to occur with inherent systemic noise using exact stochastic simulation algorithm due to Gillespie. The role of intrinsic noise in a couple of other phenomena is also discussed. PMID:21974659

  3. Advanced acousto-optic signal processors

    NASA Technical Reports Server (NTRS)

    Casasent, D.

    1983-01-01

    The basic acousto-optic signal processing architectures (spectrum analyzer, space-integrating, time-integrating, and triple product processor) systems and algorithms such as the chirp-Z transform are reviewed. New acousto-optic data processing systems and applications that utilze these basic architectures and new ones are described. These include a matched spatial filter acousto-optic processor, two new hybrid time and space-integrating systems, a triple product processor, and four new matrix-vector iterative feedback systems.

  4. Parametric Amplification For Detecting Weak Optical Signals

    NASA Technical Reports Server (NTRS)

    Hemmati, Hamid; Chen, Chien; Chakravarthi, Prakash

    1996-01-01

    Optical-communication receivers of proposed type implement high-sensitivity scheme of optical parametric amplification followed by direct detection for reception of extremely weak signals. Incorporates both optical parametric amplification and direct detection into optimized design enhancing effective signal-to-noise ratios during reception in photon-starved (photon-counting) regime. Eliminates need for complexity of heterodyne detection scheme and partly overcomes limitations imposed on older direct-detection schemes by noise generated in receivers and by limits on quantum efficiencies of photodetectors.

  5. Radio Science from an Optical Communications Signal

    NASA Technical Reports Server (NTRS)

    Moision, Bruce; Asmar, Sami; Oudrhiri, Kamal

    2013-01-01

    NASA is currently developing the capability to deploy deep space optical communications links. This creates the opportunity to utilize the optical link to obtain range, doppler, and signal intensity estimates. These may, in turn, be used to complement or extend the capabilities of current radio science. In this paper we illustrate the achievable precision in estimating range, doppler, and received signal intensity of an non-coherent optical link (the current state-of-the-art for a deep-space link). We provide a joint estimation algorithm with performance close to the bound. We draw comparisons to estimates based on a coherent radio frequency signal, illustrating that large gains in either precision or observation time are possible with an optical link.

  6. Optical Profilometers Using Adaptive Signal Processing

    NASA Technical Reports Server (NTRS)

    Hall, Gregory A.; Youngquist, Robert; Mikhael, Wasfy

    2006-01-01

    A method of adaptive signal processing has been proposed as the basis of a new generation of interferometric optical profilometers for measuring surfaces. The proposed profilometers would be portable, hand-held units. Sizes could be thus reduced because the adaptive-signal-processing method would make it possible to substitute lower-power coherent light sources (e.g., laser diodes) for white light sources and would eliminate the need for most of the optical components of current white-light profilometers. The adaptive-signal-processing method would make it possible to attain scanning ranges of the order of decimeters in the proposed profilometers.

  7. Optical coherence microscopy for deep tissue imaging of the cerebral cortex with intrinsic contrast

    NASA Astrophysics Data System (ADS)

    Srinivasan, Vivek J.; Radhakrishnan, Harsha; Jiang, James Y.; Barry, Scott; Cable, Alex E.

    2012-01-01

    We demonstrate Optical Coherence Microscopy (OCM) for in vivo imaging of the rat cerebral cortex. Imaging does not require addition of dyes or contrast agents, and is achieved through intrinsic scattering contrast and image processing alone. Furthermore, we demonstrate in vivo, quantitative measurements of optical properties and angiography in the rat cerebral cortex. Imaging depths greater than those achieved by conventional two-photon microscopy are demonstrated.

  8. Intrinsic signal imaging of brain function using a small implantable CMOS imaging device

    NASA Astrophysics Data System (ADS)

    Haruta, Makito; Sunaga, Yoshinori; Yamaguchi, Takahiro; Takehara, Hironari; Noda, Toshihiko; Sasagawa, Kiyotaka; Tokuda, Takashi; Ohta, Jun

    2015-04-01

    A brain functional imaging technique over a long period is important to understand brain functions related to animal behavior. We have developed a small implantable CMOS imaging device for measuring brain activity in freely moving animals. This device is composed of a CMOS image sensor chip and LEDs for illumination. In this study, we demonstrated intrinsic signal imaging of blood flow using the device with a green LED light source at a peak wavelength of 535 nm, which corresponds to one of the absorption spectral peaks of blood cells. Brain activity increases regional blood flow. The device light weight of about 0.02 g makes it possible to stably measure brain activity through blood flow over a long period. The device has successfully measured the intrinsic signal related to sensory stimulation on the primary somatosensory cortex.

  9. Which is the best intrinsic motivation signal for learning multiple skills?

    PubMed

    Santucci, Vieri G; Baldassarre, Gianluca; Mirolli, Marco

    2013-01-01

    Humans and other biological agents are able to autonomously learn and cache different skills in the absence of any biological pressure or any assigned task. In this respect, Intrinsic Motivations (i.e., motivations not connected to reward-related stimuli) play a cardinal role in animal learning, and can be considered as a fundamental tool for developing more autonomous and more adaptive artificial agents. In this work, we provide an exhaustive analysis of a scarcely investigated problem: which kind of IM reinforcement signal is the most suitable for driving the acquisition of multiple skills in the shortest time? To this purpose we implemented an artificial agent with a hierarchical architecture that allows to learn and cache different skills. We tested the system in a setup with continuous states and actions, in particular, with a kinematic robotic arm that has to learn different reaching tasks. We compare the results of different versions of the system driven by several different intrinsic motivation signals. The results show (a) that intrinsic reinforcements purely based on the knowledge of the system are not appropriate to guide the acquisition of multiple skills, and (b) that the stronger the link between the IM signal and the competence of the system, the better the performance.

  10. Quantitative biomarkers of human skin photoaging based on intrinsic second harmonic generation signal.

    PubMed

    Zhuo, Shuangmu; Zhu, Xiaoqin; Chen, Jianxin; Xie, Shusen

    2013-01-01

    Collagen change is a major feature in the photoaged human skin. Here, we present the use of intrinsic second harmonic generation (SHG) signal as a novel means to quantify collagen change with photoaging. We obtain the SHG images of the superficial dermis from ex vivo the cheek skin and the abdomen skin of eight patients aged 55-60 years. The results show that SHG signal can quantitatively reveal collagen change between normal and photoaged human skin in three dimensions. By comparing normal with photoaged dermis, there are significant differences in the collagen content and fine structure, providing substantial potential to be applied in vivo for the clinical diagnosis of human skin photoaging.

  11. DLP switched blaze grating: the heart of optical signal processing

    NASA Astrophysics Data System (ADS)

    Duncan, Walter M.; Lee, Benjamin L.; Rancuret, Paul; Sawyers, Bryce D.; Endsley, Lynn; Powell, Donald

    2003-01-01

    We have developed an approach for processing communication signals in the optical domain using a DLP digital mirror array driven by a Digital Signal Processor (DSP). In optical communication systems, modulation rates of 10 GB/s and above are common, hence, direct processing of Dense Wavelength Division Multiplexed (DWDM) optical signals without undergoing Optical to Electrical conversion has become a key requirement for cost effective deployment of dynamic optical networks. This work will discuss primarily applications of Optical Signal Processing (OSP) to coherent DWDM signals. Optical Signal Processing has also found applications in spectroscopy, microscopy, sensing, optical correlation, and testing.

  12. Modulation and detection of optical signals

    NASA Technical Reports Server (NTRS)

    Goodwin, F. E.

    1972-01-01

    A summary of information is presented which is related to the modulation and detection of information on optical carriers. It emphasizes the treatment of information transfer through an entire system. The most common configurations are considered: intensity modulation, amplitude modulation, frequency or phase modulation, and both direct and coherent detection. In assessing these configurations information capacity and message signal-to-noise ratio are used as a basis of comparison. The physical and geometric treatment of optical heterodyne (or coherent) detection is given.

  13. Intrinsic Disorder in Transmembrane Proteins: Roles in Signaling and Topology Prediction

    PubMed Central

    Bürgi, Jérôme; Xue, Bin; Uversky, Vladimir N.

    2016-01-01

    Intrinsically disordered regions (IDRs) are peculiar stretches of amino acids that lack stable conformations in solution. Intrinsic Disorder containing Proteins (IDP) are defined by the presence of at least one large IDR and have been linked to multiple cellular processes including cell signaling, DNA binding and cancer. Here we used computational analyses and publicly available databases to deepen insight into the prevalence and function of IDRs specifically in transmembrane proteins, which are somewhat neglected in most studies. We found that 50% of transmembrane proteins have at least one IDR of 30 amino acids or more. Interestingly, these domains preferentially localize to the cytoplasmic side especially of multi-pass transmembrane proteins, suggesting that disorder prediction could increase the confidence of topology prediction algorithms. This was supported by the successful prediction of the topology of the uncharacterized multi-pass transmembrane protein TMEM117, as confirmed experimentally. Pathway analysis indicated that IDPs are enriched in cell projection and axons and appear to play an important role in cell adhesion, signaling and ion binding. In addition, we found that IDP are enriched in phosphorylation sites, a crucial post translational modification in signal transduction, when compared to fully ordered proteins and to be implicated in more protein-protein interaction events. Accordingly, IDPs were highly enriched in short protein binding regions called Molecular Recognition Features (MoRFs). Altogether our analyses strongly support the notion that the transmembrane IDPs act as hubs in cellular signal events. PMID:27391701

  14. State-dependent firing determines intrinsic dendritic Ca2+ signaling in thalamocortical neurons.

    PubMed

    Errington, Adam C; Renger, John J; Uebele, Victor N; Crunelli, Vincenzo

    2010-11-01

    Activity-dependent dendritic Ca(2+) signals play a critical role in multiple forms of nonlinear cellular output and plasticity. In thalamocortical neurons, despite the well established spatial separation of sensory and cortical inputs onto proximal and distal dendrites, respectively, little is known about the spatiotemporal dynamics of intrinsic dendritic Ca(2+) signaling during the different state-dependent firing patterns that are characteristic of these neurons. Here we demonstrate that T-type Ca(2+) channels are expressed throughout the entire dendritic tree of rat thalamocortical neurons and that they mediate regenerative propagation of low threshold spikes, typical of, but not exclusive to, sleep states, resulting in global dendritic Ca(2+) influx. In contrast, actively backpropagating action potentials, typical of wakefulness, result in smaller Ca(2+) influxes that can temporally summate to produce dendritic Ca(2+) accumulations that are linearly related to firing frequency but spatially confined to proximal dendritic regions. Furthermore, dendritic Ca(2+) transients evoked by both action potentials and low-threshold spikes are shaped by Ca(2+) uptake by sarcoplasmic/endoplasmic reticulum Ca(2+) ATPases but do not rely on Ca(2+)-induced Ca(2+) release. Our data demonstrate that thalamocortical neurons are endowed with intrinsic dendritic Ca(2+) signaling properties that are spatially and temporally modified in a behavioral state-dependent manner and suggest that backpropagating action potentials faithfully inform proximal sensory but not distal corticothalamic synapses of neuronal output, whereas corticothalamic synapses only "detect" Ca(2+) signals associated with low-threshold spikes.

  15. Electro-optical techniques for signal conditioning

    NASA Astrophysics Data System (ADS)

    Helfrich, R. W.

    1981-01-01

    Electro-optical (EO) processing is discussed as a potential alternative to the all-digital approach to signal processing. Nonuniformity compensation can be done by normalizing all the single element detectors outputs in a staring array for both gain and level. Distortion correction can be accomplished with blackbodies, scene statistics or defocused optics. An algorithm used in digital signal conditioning that can be closely approximated by EO techniques is Local Area Brightness Control (LABC). In a digital processor, LABC is performed on a pixel-by-pixel basis, resulting in an enormous amount of calculation. A partially defocused optical system can be used in an EO analog to the digital system. For both nonuniformity compensation and LABC, the EO technique can result in great simplification.

  16. Epileptic seizure classification in EEG signals using second-order difference plot of intrinsic mode functions.

    PubMed

    Pachori, Ram Bilas; Patidar, Shivnarayan

    2014-02-01

    Epilepsy is a neurological disorder which is characterized by transient and unexpected electrical disturbance of the brain. The electroencephalogram (EEG) is a commonly used signal for detection of epileptic seizures. This paper presents a new method for classification of ictal and seizure-free EEG signals. The proposed method is based on the empirical mode decomposition (EMD) and the second-order difference plot (SODP). The EMD method decomposes an EEG signal into a set of symmetric and band-limited signals termed as intrinsic mode functions (IMFs). The SODP of IMFs provides elliptical structure. The 95% confidence ellipse area measured from the SODP of IMFs has been used as a feature in order to discriminate seizure-free EEG signals from the epileptic seizure EEG signals. The feature space obtained from the ellipse area parameters of two IMFs has been used for classification of ictal and seizure-free EEG signals using the artificial neural network (ANN) classifier. It has been shown that the feature space formed using ellipse area parameters of first and second IMFs has given good classification performance. Experimental results on EEG database available by the University of Bonn, Germany, are included to illustrate the effectiveness of the proposed method.

  17. [Determination of intrinsic alliin dissolution rates with fiber-optic sensing process analysis].

    PubMed

    Geng, Jing; Zhang, Zi-Cheng; Zhang, Hai-Bo; Li, Xin-Xia; Chen, Jian

    2014-10-01

    The apparatus for intrinsic dissolution test recorded in United States Pharmacopeia (USP) integrating with fiber-optic drug dissolution test system (FODT) were used to real-time monitor intrinsic dissolution processes of alliin in four media which were water, solution of HCl with pH 1.2, buffer solution of acetate with pH 4.5, and buffer solution of phosphate with pH 6.8. The intrinsic dissolution rate (IDR) and the similarity factor (f2) of two intrinsic dissolution curves with two apparatuses were calculated. The IDR values of alliin with rotating disk system were 28.1.3, 33.55, 28.38 and 30.95 mg x cm(-2) x min(-1) in four media, respectively. And the IDR values of alliin with stationary disk system were 44.16, 47.07, 45.11 and 51.34 mg x cm(-2) x min(-1), respectively. The similarity factors were 56.42, 50.75, 40.30 and 40.64, respectively. The results showed that the intrinsic alliin dissolution rates were much greater than 1 mg x cm(-2) x min(-1). It inferred that alliin dissolution would not be the rate limiting step to absorption. PMID:25577881

  18. Intrinsic parameterization of a computational optical system for long-distance displacement structural monitoring

    NASA Astrophysics Data System (ADS)

    Martins, Luís F. Lages; Rebordão, José Manuel N. V.; Ribeiro, Álvaro Silva

    2015-01-01

    We aim at the intrinsic parameterization of a computational optical system applied in long-distance displacement measurement of large-scale structures. In this structural-monitoring scenario, the observation distance established between the digital camera and reference targets, which is composed of the computational optical system, can range from 100 up to 1000 m, requiring the use of long-focal length lenses in order to obtain a suitable sensitivity for the three-dimensional displacement measurement of the observed structure which can be of reduced magnitude. Intrinsic parameterization of long-focal length cameras is an emergent issue since conventional approaches applied for reduced focal length cameras are not suitable mainly due to ill-conditioned matrices in least squares estimation procedures. We describe the intrinsic parameterization of a long-focal length camera (600 mm) by the diffractive optical element method and present the obtained estimates and measurement uncertainties, discussing their contribution for the system's validation by calibration field test and displacement measurement campaigns in a long-span suspension bridge.

  19. A VHDL Core for Intrinsic Evolution of Discrete Time Filters with Signal Feedback

    NASA Technical Reports Server (NTRS)

    Gwaltney, David A.; Dutton, Kenneth

    2005-01-01

    The design of an Evolvable Machine VHDL Core is presented, representing a discrete-time processing structure capable of supporting control system applications. This VHDL Core is implemented in an FPGA and is interfaced with an evolutionary algorithm implemented in firmware on a Digital Signal Processor (DSP) to create an evolvable system platform. The salient features of this architecture are presented. The capability to implement IIR filter structures is presented along with the results of the intrinsic evolution of a filter. The robustness of the evolved filter design is tested and its unique characteristics are described.

  20. Transponder for synchronization of optical signals

    SciTech Connect

    Apollonov, V.V.; Brytkov, V.V.; Zienko, S.I.; Murav'ev, S.V.; Shakir, Yu.A.

    1988-02-01

    Along with high noise immunity, electronic systems for sychronization of high-power pulsed lasers must have highly stable sync pulses. For example, delay instability of the pulses used to control a laser operating as a regenerative amplifier must not exceed approx.2-3 nsec. Even more rigid requirements are imposed on the trigger circuits of the recording and measuring apparatus. We describe a version of a delay device with optical triggering and an optical output signal. The sync-pulse delay range is 15-1500 nsec, and the accuracy and instability of the delay are less than or equal to2 nsec over the entire range.

  1. Intrinsic optical and passive electrical properties of cut frog twitch fibers

    PubMed Central

    1987-01-01

    This article describes a new apparatus for making simultaneous optical measurements on single muscle fibers at three different wavelengths and two planes of linear polarization. There are two modes of operation: mode 1 measures the individual absorbances of light linearly polarized along and perpendicular to the fiber axis, and mode 2 measures retardation (or birefringence) and the average of the two absorbance components. Although some intact frog twitch fibers were studied, most experiments used cut fibers (Hille, B., and D. T. Campbell. 1976. Journal of General Physiology. 67:265-293) mounted in a double-Vaseline- gap chamber (Kovacs, L., E. Rios, and M. F. Schneider. 1983. Journal of Physiology. 343:161-196). The end-pool segments were usually exposed for 2 min to 0.01% saponin. This procedure, used in subsequent experiments to make the external membranes in the end pools permeable to Ca indicators (Maylie, J., M. Irving, N. L. Sizto, G. Boyarsky, and W. K. Chandler. 1987. Journal of General Physiology. 89:145-176; Maylie, J., M. Irving, N. L. Sizto, and W. K. Chandler. 1987. Journal of General Physiology. 89:41-143), was routinely employed so that all our cut fiber results would be comparable. A simple method, which does not require microelectrodes, allowed continual estimation of a fiber's membrane (rm) and internal longitudinal (ri) resistances as well as the external resistance (re) under the Vaseline seals. The values of rm and ri obtained from cut fibers with this method agree reasonably well with values obtained from intact fibers using microelectrode techniques. Optical measurements were made on resting and action potential- stimulated fibers. The intrinsic fiber absorbance, defined operationally as log10 of the ratio of incident light to transmitted light intensity, was similar in intact and cut preparations, as were the changes that accompanied stimulation. On the other hand, the resting birefringence and the peak of the active change in cut fibers

  2. Optically Controlled Signal Amplification for DNA Computation.

    PubMed

    Prokup, Alexander; Hemphill, James; Liu, Qingyang; Deiters, Alexander

    2015-10-16

    The hybridization chain reaction (HCR) and fuel-catalyst cycles have been applied to address the problem of signal amplification in DNA-based computation circuits. While they function efficiently, these signal amplifiers cannot be switched ON or OFF quickly and noninvasively. To overcome these limitations, a light-activated initiator strand for the HCR, which enabled fast optical OFF → ON switching, was developed. Similarly, when a light-activated version of the catalyst strand or the inhibitor strand of a fuel-catalyst cycle was applied, the cycle could be optically switched from OFF → ON or ON → OFF, respectively. To move the capabilities of these devices beyond solution-based operations, the components were embedded in agarose gels. Irradiation with customizable light patterns and at different time points demonstrated both spatial and temporal control. The addition of a translator gate enabled a spatially activated signal to travel along a predefined path, akin to a chemical wire. Overall, the addition of small light-cleavable photocaging groups to DNA signal amplification circuits enabled conditional control as well as fast photocontrol of signal amplification. PMID:25621535

  3. Regulation of branching dynamics by axon-intrinsic asymmetries in Tyrosine Kinase Receptor signaling

    PubMed Central

    Zschätzsch, Marlen; Oliva, Carlos; Langen, Marion; De Geest, Natalie; Özel, Mehmet Neset; Williamson, W Ryan; Lemon, William C; Soldano, Alessia; Munck, Sebastian; Hiesinger, P Robin; Sanchez-Soriano, Natalia; Hassan, Bassem A

    2014-01-01

    Axonal branching allows a neuron to connect to several targets, increasing neuronal circuit complexity. While axonal branching is well described, the mechanisms that control it remain largely unknown. We find that in the Drosophila CNS branches develop through a process of excessive growth followed by pruning. In vivo high-resolution live imaging of developing brains as well as loss and gain of function experiments show that activation of Epidermal Growth Factor Receptor (EGFR) is necessary for branch dynamics and the final branching pattern. Live imaging also reveals that intrinsic asymmetry in EGFR localization regulates the balance between dynamic and static filopodia. Elimination of signaling asymmetry by either loss or gain of EGFR function results in reduced dynamics leading to excessive branch formation. In summary, we propose that the dynamic process of axon branch development is mediated by differential local distribution of signaling receptors. DOI: http://dx.doi.org/10.7554/eLife.01699.001 PMID:24755286

  4. Development and study the performance of PBA cladding modified fiber optic intrinsic biosensor for urea detection

    NASA Astrophysics Data System (ADS)

    Botewad, S. N.; Pahurkar, V. G.; Muley, G. G.

    2016-05-01

    The fabrication and study of a cladding modified fiber optic intrinsic urea biosensor based on evanescent wave absorbance has been presented. The sensor was prepared using cladding modification technique by removing a small portion of cladding of an optical fiber and modifying with an active cladding of porous polyaniline-boric acid (PBA) matrix to immobilize enzyme-urease through cross-linking via glutaraldehyde. The nature of as-synthesized and deposited PBA film on fiber optic sensing element was studied by ultraviolet-visible (UV-vis) spectroscopy and X-ray diffraction (XRD) analysis. The performance of the developed sensor was studied for different urea concentrations in solutions prepared in phosphate buffer.

  5. Fabrication of polyaniline-HCl cladding modified fiber optic intrinsic biosensor for glucose detection

    NASA Astrophysics Data System (ADS)

    Pahurkar, Vikas; Tamgadge, Yuoraj; Muley, Gajanan

    2016-05-01

    In the present study, we have fabricated and studied response of cladding modified fiber optic intrinsic glucose biosensor (FOIGB). The optical fiber was used as a light transforming waveguide and sensing element fabricated over it by applying a thin layer of polymer. The cladding of the sensor was modified with the polyaniline-hydrochloric acid (PANI-HCl) polymer matrix. The PANI-HCl matrix provides an amorphous morphology useful to immobilize glucose oxidase (GOx) biomolecules through cross-linking technique via glutaraldehyde. The present sensor was used to detect the glucose analyte in the solution. In the sensing response study of FOIGB toward glucose, novel modal power distribution (MPD) technique was used. The reaction between GOx and glucose changes the optical properties of prepared FOIGB and hence modify MPD at output as a function of glucose concentration. The nature and surface morphology of PANI-HCl matrix has been studied.

  6. Emission spectra and intrinsic optical bistability in a two-level medium

    NASA Astrophysics Data System (ADS)

    Gladush, M. G.; Kuznetsov, D. V.; Roerich, Vl. K.

    2011-10-01

    Scattering of resonant radiation in a dense two-level medium is studied theoretically accounting for local field effects and renormalisation of the resonance frequency. Intrinsic optical bistability is viewed as switching between different spectral patterns of fluorescent light controlled by the incident field strength. Response spectra are calculated analytically for the entire hysteresis loop of atomic excitation. The equations to describe the non-linear interaction of an atomic ensemble with light are derived from the Bogolubov-Born-Green-Kirkwood-Yvon hierarchy for reduced single particle density matrices of atoms and quantised field modes and their correlation operators. The spectral power of scattered light with separated coherent and incoherent constituents is obtained straightforwardly within the hierarchy. The formula obtained for emission spectra can be used to distinguish between possible mechanisms suggested to produce intrinsic bistability in experiments.

  7. Intraoperative optical biopsy for brain tumors using spectro-lifetime properties of intrinsic fluorophores

    NASA Astrophysics Data System (ADS)

    Vasefi, Fartash; Kittle, David S.; Nie, Zhaojun; Falcone, Christina; Patil, Chirag G.; Chu, Ray M.; Mamelak, Adam N.; Black, Keith L.; Butte, Pramod V.

    2016-04-01

    We have developed and tested a system for real-time intra-operative optical identification and classification of brain tissues using time-resolved fluorescence spectroscopy (TRFS). A supervised learning algorithm using linear discriminant analysis (LDA) employing selected intrinsic fluorescence decay temporal points in 6 spectral bands was employed to maximize statistical significance difference between training groups. The linear discriminant analysis on in vivo human tissues obtained by TRFS measurements (N = 35) were validated by histopathologic analysis and neuronavigation correlation to pre-operative MRI images. These results demonstrate that TRFS can differentiate between normal cortex, white matter and glioma.

  8. Polymer optical fiber Bragg grating acting as an intrinsic biochemical concentration sensor.

    PubMed

    Zhang, Wei; Webb, David; Peng, Gangding

    2012-04-15

    We demonstrate an intrinsic biochemical concentration sensor based on a polymer optical fiber Bragg grating. The water content absorbed by the polymer fiber from a surrounding solution depends on the concentration of the solution because of the osmotic effect. The variation of water content in the fiber causes a change in the fiber dimensions and a variation in refractive index and, therefore, a shift in the Bragg wavelength. Saline solutions with concentration from 0% to 22% were used to demonstrate the sensing principle, resulting in a total wavelength shift of 0.9 nm, allowing high-resolution concentration measurements to be realized.

  9. Extrinsic and intrinsic signals converge on the Runx1/CBFβ transcription factor for nonpeptidergic nociceptor maturation

    PubMed Central

    Huang, Siyi; O'Donovan, Kevin J; Turner, Eric E; Zhong, Jian; Ginty, David D

    2015-01-01

    The generation of diverse neuronal subtypes involves specification of neural progenitors and, subsequently, postmitotic neuronal differentiation, a relatively poorly understood process. Here, we describe a mechanism whereby the neurotrophic factor NGF and the transcription factor Runx1 coordinate postmitotic differentiation of nonpeptidergic nociceptors, a major nociceptor subtype. We show that the integrity of a Runx1/CBFβ holocomplex is crucial for NGF-dependent nonpeptidergic nociceptor maturation. NGF signals through the ERK/MAPK pathway to promote expression of Cbfb but not Runx1 prior to maturation of nonpeptidergic nociceptors. In contrast, transcriptional initiation of Runx1 in nonpeptidergic nociceptor precursors is dependent on the homeodomain transcription factor Islet1, which is largely dispensable for Cbfb expression. Thus, an NGF/TrkA-MAPK-CBFβ pathway converges with Islet1-Runx1 signaling to promote Runx1/CBFβ holocomplex formation and nonpeptidergic nociceptor maturation. Convergence of extrinsic and intrinsic signals to control heterodimeric transcription factor complex formation provides a robust mechanism for postmitotic neuronal subtype specification. DOI: http://dx.doi.org/10.7554/eLife.10874.001 PMID:26418744

  10. Intrinsic excitability state of local neuronal population modulates signal propagation in feed-forward neural networks.

    PubMed

    Han, Ruixue; Wang, Jiang; Yu, Haitao; Deng, Bin; Wei, Xilei; Qin, Yingmei; Wang, Haixu

    2015-04-01

    Reliable signal propagation across distributed brain areas is an essential requirement for cognitive function, and it has been investigated extensively in computational studies where feed-forward network (FFN) is taken as a generic model. But it is still unclear how distinct local network states, which are intrinsically generated by synaptic interactions within each layer, would affect the ability of FFN to transmit information. Here we investigate the impact of such network states on propagating transient synchrony (synfire) and firing rate by a combination of numerical simulations and analytical approach. Specifically, local network dynamics is attributed to the competition between excitatory and inhibitory neurons within each layer. Our results show that concomitant with different local network states, the performance of signal propagation differs dramatically. For both synfire propagation and firing rate propagation, there exists an optimal local excitability state, respectively, that optimizes the performance of signal propagation. Furthermore, we find that long-range connections strongly change the dependence of spiking activity propagation on local network state and propose that these two factors work jointly to determine information transmission across distributed networks. Finally, a simple mean field approach that bridges response properties of long-range connectivity and local subnetworks is utilized to reveal the underlying mechanism.

  11. Intrinsic excitability state of local neuronal population modulates signal propagation in feed-forward neural networks

    NASA Astrophysics Data System (ADS)

    Han, Ruixue; Wang, Jiang; Yu, Haitao; Deng, Bin; Wei, Xilei; Qin, Yingmei; Wang, Haixu

    2015-04-01

    Reliable signal propagation across distributed brain areas is an essential requirement for cognitive function, and it has been investigated extensively in computational studies where feed-forward network (FFN) is taken as a generic model. But it is still unclear how distinct local network states, which are intrinsically generated by synaptic interactions within each layer, would affect the ability of FFN to transmit information. Here we investigate the impact of such network states on propagating transient synchrony (synfire) and firing rate by a combination of numerical simulations and analytical approach. Specifically, local network dynamics is attributed to the competition between excitatory and inhibitory neurons within each layer. Our results show that concomitant with different local network states, the performance of signal propagation differs dramatically. For both synfire propagation and firing rate propagation, there exists an optimal local excitability state, respectively, that optimizes the performance of signal propagation. Furthermore, we find that long-range connections strongly change the dependence of spiking activity propagation on local network state and propose that these two factors work jointly to determine information transmission across distributed networks. Finally, a simple mean field approach that bridges response properties of long-range connectivity and local subnetworks is utilized to reveal the underlying mechanism.

  12. B cell-intrinsic TLR7 signaling is essential for the development of spontaneous germinal centers.

    PubMed

    Soni, Chetna; Wong, Eric B; Domeier, Phillip P; Khan, Tahsin N; Satoh, Takashi; Akira, Shizuo; Rahman, Ziaur S M

    2014-11-01

    Spontaneous germinal center (Spt-GC) B cells and follicular helper T cells generate high-affinity autoantibodies that are involved in the development of systemic lupus erythematosus. TLRs play a pivotal role in systemic lupus erythematosus pathogenesis. Although previous studies focused on the B cell-intrinsic role of TLR-MyD88 signaling on immune activation, autoantibody repertoire, and systemic inflammation, the mechanisms by which TLRs control the formation of Spt-GCs remain unclear. Using nonautoimmune C57BL/6 (B6) mice deficient in MyD88, TLR2, TLR3, TLR4, TLR7, or TLR9, we identified B cell-intrinsic TLR7 signaling as a prerequisite to Spt-GC formation without the confounding effects of autoimmune susceptibility genes and the overexpression of TLRs. TLR7 deficiency also rendered autoimmune B6.Sle1b mice unable to form Spt-GCs, leading to markedly decreased autoantibodies. Conversely, B6.yaa and B6.Sle1b.yaa mice expressing an extra copy of TLR7 and B6.Sle1b mice treated with a TLR7 agonist had increased Spt-GCs and follicular helper T cells. Further, TLR7/MyD88 deficiency led to compromised B cell proliferation and survival after B cell stimulation both in vitro and in vivo. In contrast, TLR9 inhibited Spt-GC development. Our findings demonstrate an absolute requirement for TLR7 and a negative regulatory function for TLR9 in Spt-GC formation under nonautoimmune and autoimmune conditions. Our data suggest that, under nonautoimmune conditions, Spt-GCs initiated by TLR7 produce protective Abs. However, in the presence of autoimmune susceptibility genes, TLR7-dependent Spt-GCs produce pathogenic autoantibodies. Thus, a single copy of TLR7 in B cells is the minimal requirement for breaking the GC-tolerance checkpoint.

  13. Acousto-optic filtering of lidar signals

    NASA Technical Reports Server (NTRS)

    Kolarov, G.; Deleva, A.; Mitsev, TS.

    1992-01-01

    The predominant part of the noise in lidar receivers is created by the background radiation; therefore, one of the most important elements of the receiving optics is a spectrally selecting filter placed in front of the photodetector. Interference filters are usually used to transmit a given wavelength. Specific properties of the interference filters, such as simple design, reliability, small size, and large aperture, combined with high transmission coefficient and narrow spectral band, make them the preferred spectral device in many cases. However, problems arise in applications such as the Differential Absorption Lidar (DIAL) technique, where fast tuning within a wide spectral region is necessary. Tunable acousto-optical filters (TAOF), used recently in astrophysical observations to suppress the background radiation, can be employed with success in lidar sounding. They are attractive due to the possibility for fast spectral scanning with a narrow transmission band. The TAOF's advantages are fully evident in DIAL lidars where one must simultaneously receive signals at two laser frequencies.

  14. Intrinsic fiber optic absorption sensor for the detection of volatile organic compounds

    NASA Astrophysics Data System (ADS)

    Klunder, Gregory L.; Silva, Robert J.; Russo, Richard E.

    1994-03-01

    A core-based intrinsic fiber optic absorption sensor has been developed for the detection of volatile organic compounds. The sensor can detect organics in aqueous solutions or in the vapor phase without a chemical reaction. The distal ends of transmission and receiving fibers are connected by a small section of an optically clear silicone rubber. The silicone rubber section acts both as a lightpipe and as a selective membrane into which the analyte molecules can diffuse. Absorption spectra obtained in the nearinfrared (NIR) provide qualitative and quantitative information about the analyte. Water, which has strong broadband absorption in aqueous solutions of the NIR, is excluded from the spectra due to the hydrophobic properties of the silicone rubber. In a stirred solution, the sensor reaches equilibrium in approximately 10 minutes. The current limit of detection is 1.0 ppm for TCE in an aqueous solution.

  15. Inverted optical intrinsic response accompanied by decreased cerebral blood flow are related to both neuronal inhibition and excitation

    PubMed Central

    Ma, Zengguang; Cao, Pengjia; Sun, Pengcheng; Zhao, Linna; Li, Liming; Tong, Shanbao; Lu, Yiliang; Yan, Yan; Chen, Yao; Chai, Xinyu

    2016-01-01

    Negative hemodynamic response has been widely reported in blood oxygenation level-dependent (BOLD) functional magnetic resonance imaging studies, however its origin is still controversial. Optical intrinsic signal (OIS) imaging can be used to study brain activity by simultaneously recording hemodynamic signals at different wavelengths with high spatial resolution. In this study, we found transcorneal electrical stimulation (TcES) could elicit both positive OIS response (POR) and negative OIS response (NOR) in cats’ visual cortex. We then investigated the property of this negative response to TcES and its relationship with cerebral blood flow (CBF) and neuronal activity. Results from laser speckle contrast imaging showed decreased CBF in the NOR region while increased CBF in the POR region. Both planar and laminar electrophysiological recordings in the middle (500–700 μm) cortical layers demonstrated that decreased and increased neuronal activities were coexisted in the NOR region. Furthermore, decreased neuronal activity was also detected in the deep cortical layers in the NOR region. This work provides evidence that the negative OIS together with the decreased CBF should be explained by mechanisms of both neuronal inhibition and excitation within middle cortical layers. Our results would be important for interpreting neurophysiological mechanisms underlying the negative BOLD signals. PMID:26860040

  16. High-definition optical coherence tomography intrinsic skin ageing assessment in women: a pilot study.

    PubMed

    Boone, M A L M; Suppa, M; Marneffe, A; Miyamoto, M; Jemec, G B E; Del Marmol, V

    2015-10-01

    Several non-invasive two-dimensional techniques with different lateral resolution and measurable depth range have proved to be useful in assessing and quantifying morphological changes in skin ageing. Among these, only in vivo microscopy techniques permit histometric measurements in vivo. Qualitative and quantitative assessment of chronological (intrinsic) age-related (IAR) morphological changes of epidermis, dermo-epidermal junction (DEJ), papillary dermis (PD), papillary-reticular dermis junction and reticular dermis (RD) have been performed by high-definition optical coherence tomography in real time 3-D. HD-OCT images were taken at the internal site of the right upper arm. Qualitative HD-OCT IAR descriptors were reported at skin surface, at epidermal layer, DEJ, PD and upper RD. Quantitative evaluation of age-related compaction and backscattered intensity or brightness of different skin layers was performed by using the plugin plot z-axis profile of ImageJ(®) software permitting intensity assessment of HD-OCT (DICOM) images (3-D images). Analysis was in blind from all clinical information. Sixty, fair-skinned (Fitzpatrick types I-III) healthy females were analysed retrospectively in this study. The subjects belonged to three age groups: twenty in group I aged 20-39, twenty in group II aged 40-59 and twenty in group III aged 60-79. Only intrinsic ageing in women has been studied. Significant age-related qualitative and quantitative differences could be noticed. IAR changes in dermal matrix fibers morphology/organisation and in microvasculature were observed. The brightness and compaction of the different skin layers increased significantly with intrinsic skin ageing. The depth of visibility of fibers in RD increased significantly in the older age group. In conclusion, HD-OCT allows 3-D in vivo and real time qualitative and quantitative assessment of chronological (intrinsic) age-related morphological skin changes at high resolution from skin surface to a depth

  17. Effect of intrinsic organic carbon on the optical properties of fresh diesel soot

    PubMed Central

    Adler, Gabriella; Riziq, Ali Abo; Erlick, Carynelisa; Rudich, Yinon

    2010-01-01

    This study focuses on the retrieval of the normalized mass absorption cross section (MAC) of soot using theoretical calculations that incorporate new measurements of the optical properties of organic carbon (OC) intrinsic to fresh diesel soot. Intrinsic OC was extracted by water and an organic solvent, and the complex refractive index of the extracted OC was derived at 532 and 355-nm wavelengths using cavity ring-down aerosol spectrometry. The extracted OC was found to absorb weakly in the visible wavelengths and moderately at blue wavelengths. The mass ratio of OC and elemental carbon (EC) in the collected particles was evaluated using a thermo-optical method. The measured EC/OC ratio in the soot exhibited substantial variability from measurement to measurement, ranging between 2 and 5. To test the sensitivity of the MAC to this variability, three different EC/OC ratios (2∶1, 1∶1, and 1∶2) were chosen as representative. Particle size and spherule morphology were estimated using scanning electron microscopy, and the soot was found to be primarily in the form of aggregates with a dominant aggregate diameter mode in the range 200–250 nm. The measured refractive index of the extracted OC was used with a variety of theoretical models to calculate the MAC of internally mixed diesel soot at 532 and 355 nm. We conclude that Rayleigh–Debye–Gans theory on clusters of coated spherules and T-matrix of a solid EC spheroid coated by intrinsic OC are both consistent with previous measurements; however, Rayleigh–Debye–Gans theory provides a more realistic physical model for the calculation PMID:20018649

  18. Specialized optical fiber sensor for nondestructive intrinsic quality measurement of Averrhoa Carambola

    NASA Astrophysics Data System (ADS)

    Omar, Ahmad Fairuz; Matjafri, Mohd Zubir

    2013-09-01

    This paper presents an innovative and low-cost approach for nondestructive fruit quality analysis. The specialized optical fiber sensor developed and presented in this paper used a monochromatic wavelength, rather than a broad spectrum, to measure the intact carambola (star fruit) intrinsic quality, namely pH and firmness. The main objective of this research was to investigate the two optical fiber sensors used in this work, namely, the optical fiber red system (OF-RS) that operated with the peak sensitivity at 635 nm and the optical fiber near the infrared spectroscopy system (OF-NIRS) that operated with the peak sensitivity at 880 nm. Both systems showed good accuracy in the pH and firmness measurement of the intact carambola with the correlation coefficient R over 0.75, and the measurement results were comparable with those of the commercial spectrometer. The best measurement results were obtained using OF-RS (pH: R = 0.876; the root mean square error ( RMSE) = 0.211 pH; firmness: R = 0.872; RMSE = 0.909 kgf).

  19. Intrinsic Fiber Optic Chemical Sensors for Subsurface Detection of CO2

    SciTech Connect

    Alonso, Jesus

    2016-01-01

    Intelligent Optical Systems, Inc. has developed distributed intrinsic fiber optic sensors to directly quantify the concentration of dissolved or gas-phase CO2 for leak detection or plume migration in carbon capture and sequestration (CCS). The capability of the sensor for highly sensitive detection of CO2 in the pressure and temperature range of 15 to 2,000 psi and 25°C to 175°C was demonstrated, as was the capability of operating in highly corrosive and contaminated environments such as those often found in CO2 injection sites. The novel sensor system was for the first time demonstrated deployed in a deep well, detecting multiple CO2 releases, in real time, at varying depths. Early CO2 release detection, by means of a sensor cable integrating multiple sensor segments, was demonstrated, as was the capability of quantifying the leak. The novel fiber optic sensor system exhibits capabilities not achieved by any other monitoring technology. This project represents a breakthrough in monitoring capabilities for CCS applications.

  20. System and methods for determining masking signals for applying empirical mode decomposition (EMD) and for demodulating intrinsic mode functions obtained from application of EMD

    DOEpatents

    Senroy, Nilanjan; Suryanarayanan, Siddharth

    2011-03-15

    A computer-implemented method of signal processing is provided. The method includes generating one or more masking signals based upon a computed Fourier transform of a received signal. The method further includes determining one or more intrinsic mode functions (IMFs) of the received signal by performing a masking-signal-based empirical mode decomposition (EMD) using the at least one masking signal.

  1. B cell IFN-γ receptor signaling promotes autoimmune germinal centers via cell-intrinsic induction of BCL-6

    PubMed Central

    Jackson, Shaun W.; Jacobs, Holly M.; Arkatkar, Tanvi; Dam, Elizabeth M.; Scharping, Nicole E.; Kolhatkar, Nikita S.; Hou, Baidong; Buckner, Jane H.

    2016-01-01

    Dysregulated germinal center (GC) responses are implicated in the pathogenesis of human autoimmune diseases, including systemic lupus erythematosus (SLE). Although both type 1 and type 2 interferons (IFNs) are involved in lupus pathogenesis, their respective impacts on the establishment of autoimmune GCs has not been addressed. In this study, using a chimeric model of B cell-driven autoimmunity, we demonstrate that B cell type 1 IFN receptor signals accelerate, but are not required for, lupus development. In contrast, B cells functioning as antigen-presenting cells initiate CD4+ T cell activation and IFN-γ production, and strikingly, B cell–intrinsic deletion of the IFN-γ receptor (IFN-γR) abrogates autoimmune GCs, class-switched autoantibodies (auto-Abs), and systemic autoimmunity. Mechanistically, although IFN-γR signals increase B cell T-bet expression, B cell–intrinsic deletion of T-bet exerts an isolated impact on class-switch recombination to pathogenic auto-Ab subclasses without impacting GC development. Rather, in both mouse and human B cells, IFN-γ synergized with B cell receptor, toll-like receptor, and/or CD40 activation signals to promote cell-intrinsic expression of the GC master transcription factor, B cell lymphoma 6 protein. Our combined findings identify a novel B cell–intrinsic mechanism whereby IFN signals promote lupus pathogenesis, implicating this pathway as a potential therapeutic target in SLE. PMID:27069113

  2. Direct inscription of intrinsic Fabry-Perot interferometers in optical fiber tapers with a femtosecond laser

    NASA Astrophysics Data System (ADS)

    Li, Jinlong; Zhang, Xiaobei; Wang, Wenyuan; Pang, Fufei; Liu, Yunqi; Wang, Tingyun

    2011-12-01

    In this work, we report a sensing configuration of the fiber taper intrinsic Fabry-Perot interferometer directly inscribed in single-mode optical fiber tapers with different waist diameters from 14 to 80 μm using a femtosecond laser micromachining system. By controlling the inscribing depth and intensity of the fs laser pulse, the fringe visibility can exceed 9.0 dB when the fiber taper waist diameter is around 15 μm, which is sufficient for most sensing applications. The sensor sensitivity depends on the fiber taper waist diameter, while a smaller diameter corresponds to a large sensitivity. Different free spectral ranges can be achieved for various cavity lengths. Such a structure can combine the high sensitivity properties of fiber taper sensors with the high resolution features of Fabry-Perot interferometer sensors. Meanwhile, this structure can have a number of outstanding advantages, such as its small size, unique geometry, easy fabrication, low cost and capability for mass production. These fiber taper intrinsic Fabry-Perot interferometer sensors have high potential in fast detection and high precision measurement while maintaining superior reliability for chemical and biological sensing.

  3. Intrinsic protein flexibility in regulation of cell proliferation: advantages for signaling and opportunities for novel therapeutics.

    PubMed

    Follis, Ariele Viacava; Galea, Charles A; Kriwacki, Richard W

    2012-01-01

    It is now widely recognized that intrinsically disordered (or unstructured) proteins (IDPs, or IUPs) are found in organisms from all kingdoms of life. In eukaryotes, IDPs are highly abundant and perform a wide range of biological functions, including regulation and signaling. Despite increased interest in understanding the structural biology of IDPs, questions remain regarding the mechanisms through which disordered proteins perform their biological function(s). In other words, what are the relationships between disorder and function for IDPs? Several excellent reviews have recently been published that discuss the structural properties of IDPs.1-3 Here, we discuss two IDP systems which illustrate features of dynamic complexes. In the first section, we discuss two IDPs, p21 and p27, which regulate the mammalian cell division cycle by inhibiting cyclin-dependent kinases (Cdks). In the second section, we discuss recent results from Follis, Hammoudeh, Metallo and coworkers demonstrating that the IDP Myc can be bound and inhibited by small molecules through formation of dynamic complexes. Previous studies have shown that polypeptide segments of p21 and p27 are partially folded in isolation and fold further upon binding their biological targets. Interestingly, some portions of p27 which bind to and inhibit Cdk2/cyclin A remain flexible in the bound complex. This residual flexibility allows otherwise buried tyrosine residues within p27 to be phosphorylated by nonreceptor tyrosine kinases (NRTKs). Tyrosine phosphorylation relieves kinase inhibition, triggering Cdk2-mediated phosphorylation of a threonine residue within the flexible C-terminus of p27. This, in turn, marks p27 for ubiquitination and proteasomal degradation, unleashing full Cdk2 activity which drives cell cycle progression. p27, thus, constitutes a conduit for transmission of proliferative signals via posttranslational modifications. Importantly, activation of the p27 signaling conduit by oncogenic NRTKs

  4. Intrinsic heating in optically trapped Au nanoparticles measured by dark-field spectroscopy

    PubMed Central

    Andres-Arroyo, Ana; Wang, Fan; Toe, Wen Jun; Reece, Peter

    2015-01-01

    Assessing the degree of heating present when a metal nanoparticle is trapped in an optical tweezers is critical for its appropriate use in biological applications as a nanoscale force sensor. Heating is necessarily present for trapped plasmonic particles because of the non-negligible extinction which contributes to an enhanced polarisability. We present a robust method for characterising the degree of heating of trapped metallic nanoparticles, using the intrinsic temperature dependence of the localised surface plasmon resonance (LSPR) to infer the temperature of the surrounding fluid at different incident laser powers. These particle specific measurements can be used to infer the rate of heating and local temperature of trapped nanoparticles. Our measurements suggest a considerable amount of a variability in the degree of heating, on the range of 414–673 K/W, for different 100 nm diameter Au nanoparticles, and we associated this with variations in the axial trapping position. PMID:26417530

  5. Intrinsic TGF-β signaling promotes age-dependent CD8+ T cell polyfunctionality attrition

    PubMed Central

    Bhadra, Rajarshi; Moretto, Magali M.; Castillo, Julio C.; Petrovas, Constantinos; Ferrando-Martinez, Sara; Shokal, Upasana; Leal, Manuel; Koup, Richard A.; Eleftherianos, Ioannis; Khan, Imtiaz A.

    2014-01-01

    Advanced age is associated with immune system deficits that result in an increased susceptibility to infectious diseases; however, specific mediators of age-dependent immune dysfunction have not been fully elucidated. Here we demonstrated that aged mice exhibit poor effector CD8+ T cell polyfunctionality, primarily due to CD8+ T cell–extrinsic deficits, and that reduced CD8+ T cell polyfunctionality correlates with increased susceptibility to pathogenic diseases. In aged animals challenged with the parasite Encephalitozoon cuniculi, effector CD8+ T cell survival and polyfunctionality were suppressed by highly elevated TGF-β1. Furthermore, TGF-β depletion reduced effector CD8+ T cell apoptosis in both young and aged mice and enhanced effector CD8+ T cell polyfunctionality in aged mice. Surprisingly, intrinsic blockade of TGF-β signaling in CD8+ T cells was sufficient to rescue polyfunctionality in aged animals. Together, these data demonstrate that low levels of TGF-β1 promote apoptosis of CD8+ effector T cells and high TGF-β1 levels associated with age result in both CD8+ T cell apoptosis and an altered transcriptional profile, which correlates with loss of polyfunctionality. Furthermore, elevated TGF-β levels are observed in the elderly human population and in aged Drosophila, suggesting that TGF-β represents an evolutionarily conserved negative regulator of the immune response in aging organisms. PMID:24762437

  6. Optimizing the Intrinsic Signal-to-Noise Ratio of MRI Strip Detectors

    PubMed Central

    Kumar, Ananda; Bottomley, Paul A.

    2007-01-01

    An MRI detector is formed from a conducting strip separated by a dielectric substrate from a ground plane, and tuned to a quarter-wavelength. By distributing discrete tuning elements along the strip, the geometric design may be adjusted to optimize the signal-to-noise ratio (SNR) for a given application. Here a numerical electromagnetic (EM) method of moments (MoM) is applied to determine the length, width, substrate thickness, dielectric constant, and number of tuning elements that yield the best intrinsic SNR (ISNR) of the strip detector at 1.5 Tesla. The central question of how strip performance compares with that of a conventional optimized loop coil is also addressed. The numerical method is validated against the known ISNR performance of loop coils, and its ability to predict the tuning capacitances and performance of seven experimental strip detectors of varying length, width, substrate thickness, and dielectric constant. We find that strip detectors with low-dielectric constant, moderately thin-substrate, and length about 1.3 (±0.2) times the depth of interest perform best. The ISNR of strips is comparable to that of loops (i.e., higher close to the detector but lower at depth). The SNR improves with two inherently-decoupled strips, whose sensitivity profile is well-suited to parallel MRI. The findings are summarized as design “rules of thumb.” PMID:16724302

  7. Evaluation of intrinsic respiratory signal determination methods for 4D CBCT adapted for mice

    SciTech Connect

    Martin, Rachael; Pan, Tinsu; Rubinstein, Ashley; Court, Laurence; Ahmad, Moiz

    2015-01-15

    Purpose: 4D CT imaging in mice is important in a variety of areas including studies of lung function and tumor motion. A necessary step in 4D imaging is obtaining a respiratory signal, which can be done through an external system or intrinsically through the projection images. A number of methods have been developed that can successfully determine the respiratory signal from cone-beam projection images of humans, however only a few have been utilized in a preclinical setting and most of these rely on step-and-shoot style imaging. The purpose of this work is to assess and make adaptions of several successful methods developed for humans for an image-guided preclinical radiation therapy system. Methods: Respiratory signals were determined from the projection images of free-breathing mice scanned on the X-RAD system using four methods: the so-called Amsterdam shroud method, a method based on the phase of the Fourier transform, a pixel intensity method, and a center of mass method. The Amsterdam shroud method was modified so the sharp inspiration peaks associated with anesthetized mouse breathing could be detected. Respiratory signals were used to sort projections into phase bins and 4D images were reconstructed. Error and standard deviation in the assignment of phase bins for the four methods compared to a manual method considered to be ground truth were calculated for a range of region of interest (ROI) sizes. Qualitative comparisons were additionally made between the 4D images obtained using each of the methods and the manual method. Results: 4D images were successfully created for all mice with each of the respiratory signal extraction methods. Only minimal qualitative differences were noted between each of the methods and the manual method. The average error (and standard deviation) in phase bin assignment was 0.24 ± 0.08 (0.49 ± 0.11) phase bins for the Fourier transform method, 0.09 ± 0.03 (0.31 ± 0.08) phase bins for the modified Amsterdam shroud method, 0

  8. Optical Signal Processing: Poisson Image Restoration and Shearing Interferometry

    NASA Technical Reports Server (NTRS)

    Hong, Yie-Ming

    1973-01-01

    Optical signal processing can be performed in either digital or analog systems. Digital computers and coherent optical systems are discussed as they are used in optical signal processing. Topics include: image restoration; phase-object visualization; image contrast reversal; optical computation; image multiplexing; and fabrication of spatial filters. Digital optical data processing deals with restoration of images degraded by signal-dependent noise. When the input data of an image restoration system are the numbers of photoelectrons received from various areas of a photosensitive surface, the data are Poisson distributed with mean values proportional to the illuminance of the incoherently radiating object and background light. Optical signal processing using coherent optical systems is also discussed. Following a brief review of the pertinent details of Ronchi's diffraction grating interferometer, moire effect, carrier-frequency photography, and achromatic holography, two new shearing interferometers based on them are presented. Both interferometers can produce variable shear.

  9. Reconfigurable Optical Signal Processing Based on a Distributed Feedback Semiconductor Optical Amplifier

    PubMed Central

    Li, Ming; Deng, Ye; Tang, Jian; Sun, Shuqian; Yao, Jianping; Azaña, José; Zhu, Ninghua

    2016-01-01

    All-optical signal processing has been considered a solution to overcome the bandwidth and speed limitations imposed by conventional electronic-based systems. Over the last few years, an impressive range of all-optical signal processors have been proposed, but few of them come with reconfigurability, a feature highly needed for practical signal processing applications. Here we propose and experimentally demonstrate an analog optical signal processor based on a phase-shifted distributed feedback semiconductor optical amplifier (DFB-SOA) and an optical filter. The proposed analog optical signal processor can be reconfigured to perform signal processing functions including ordinary differential equation solving and temporal intensity differentiation. The reconfigurability is achieved by controlling the injection currents. Our demonstration provitdes a simple and effective solution for all-optical signal processing and computing. PMID:26813252

  10. Reconfigurable Optical Signal Processing Based on a Distributed Feedback Semiconductor Optical Amplifier.

    PubMed

    Li, Ming; Deng, Ye; Tang, Jian; Sun, Shuqian; Yao, Jianping; Azaña, José; Zhu, Ninghua

    2016-01-27

    All-optical signal processing has been considered a solution to overcome the bandwidth and speed limitations imposed by conventional electronic-based systems. Over the last few years, an impressive range of all-optical signal processors have been proposed, but few of them come with reconfigurability, a feature highly needed for practical signal processing applications. Here we propose and experimentally demonstrate an analog optical signal processor based on a phase-shifted distributed feedback semiconductor optical amplifier (DFB-SOA) and an optical filter. The proposed analog optical signal processor can be reconfigured to perform signal processing functions including ordinary differential equation solving and temporal intensity differentiation. The reconfigurability is achieved by controlling the injection currents. Our demonstration provitdes a simple and effective solution for all-optical signal processing and computing.

  11. Neuromorphic opto-electronic integrated circuits for optical signal processing

    NASA Astrophysics Data System (ADS)

    Romeira, B.; Javaloyes, J.; Balle, S.; Piro, O.; Avó, R.; Figueiredo, J. M. L.

    2014-08-01

    The ability to produce narrow optical pulses has been extensively investigated in laser systems with promising applications in photonics such as clock recovery, pulse reshaping, and recently in photonics artificial neural networks using spiking signal processing. Here, we investigate a neuromorphic opto-electronic integrated circuit (NOEIC) comprising a semiconductor laser driven by a resonant tunneling diode (RTD) photo-detector operating at telecommunication (1550 nm) wavelengths capable of excitable spiking signal generation in response to optical and electrical control signals. The RTD-NOEIC mimics biologically inspired neuronal phenomena and possesses high-speed response and potential for monolithic integration for optical signal processing applications.

  12. All-optical signal processing using dynamic Brillouin gratings

    PubMed Central

    Santagiustina, Marco; Chin, Sanghoon; Primerov, Nicolay; Ursini, Leonora; Thévenaz, Luc

    2013-01-01

    The manipulation of dynamic Brillouin gratings in optical fibers is demonstrated to be an extremely flexible technique to achieve, with a single experimental setup, several all-optical signal processing functions. In particular, all-optical time differentiation, time integration and true time reversal are theoretically predicted, and then numerically and experimentally demonstrated. The technique can be exploited to process both photonic and ultra-wide band microwave signals, so enabling many applications in photonics and in radio science. PMID:23549159

  13. An Intrinsic Fiber-Optic Sensor for Structure Lightning Current Measurement

    NASA Technical Reports Server (NTRS)

    Nguyen, Truong X.; Ely, Jay J.; Szatkowski, George N.; Mata, Carlos T.; Mata, Angel. G.; Snyder, Gary P.

    2014-01-01

    An intrinsic optical-fiber sensor based on Faraday Effect is developed that is highly suitable for measuring lightning current on aircraft, towers and complex structures. Originally developed specifically for aircraft installations, it is light-weight, non-conducting, structure conforming, and is immune to electromagnetic interference, hysteresis and saturation. It can measure total current down to DC. When used on lightning towers, the sensor can help validate other sensors and lightning detection network measurements. Faraday Effect causes light polarization to rotate when the fiber is exposed to a magnetic field in the direction of light propagation. Thus, the magnetic field strength can be determined from the light polarization change. By forming closed fiber loops and applying Ampere's law, measuring the total light rotation yields the total current enclosed. A broadband, dual-detector, reflective polarimetric scheme allows measurement of both DC component and AC waveforms with a 60 dB dynamic range. Two systems were built that are similar in design but with slightly different sensitivities. The 1310nm laser system can measure 300 A - 300 kA, and has a 15m long sensing fiber. It was used in laboratory testing, including measuring current on an aluminum structure simulating an aircraft fuselage or a lightning tower. High current capabilities were demonstrated up to 200 kA at a lightning test facility. The 1550nm laser system can measure 400 A - 400 kA and has a 25m fiber length. Used in field measurements, excellent results were achieved in the summer of 2012 measuring rocket-triggered lightning at the International Center for Lightning Research and Testing (ICLRT), Camp Blanding, Florida. In both systems increased sensitivity can be achieved with multiple fiber loops. The fiber optic sensor provides many unique capabilities not currently possible with traditional sensors. It represents an important new tool for lightning current measurement where low weight

  14. Intrinsic Optical Activity and Environmental Perturbations: Solvation Effects in Chiral Building Blocks

    NASA Astrophysics Data System (ADS)

    Lemler, Paul M.; Vaccaro, Patrick

    2016-06-01

    The non-resonant interaction of electromagnetic radiation with an isotropic ensemble of chiral molecules, which causes the incident state of linear polarization to undergo a signed rotation, long has served as a metric for gauging the enantiomeric purity of asymmetric syntheses. While the underlying phenomenon of circular birefringence (CB) typically is probed in the condensed phase, recent advances in ultrasensitive circular-differential detection schemes, as exemplified by the techniques of Cavity Ring-Down Polarimetry (CRDP), have permitted the first quantitative analyses of such processes to be performed in rarefied media. Efforts to extend vapor-phase investigations of CB to new families of chiral substrates will be discussed, with particular emphasis directed towards the elucidation of intrinsic (e.g., solvent-free) properties and their mediation by environmental perturbations (e.g., solvation). Specific species targeted by this work will include the stereoselective building blocks phenylpropylene oxide and α-methylbenzyl amine, both of which exhibit pronounced solvent-dependent changes in measured optical activity. The nature of chiroptical response in different environments will be highlighted, with quantum-chemical calculations serving to unravel the structural and electronic provenance of observed behavior.

  15. Optical bistability: A novel approach to optical signal processing and communications

    NASA Technical Reports Server (NTRS)

    Peyghambarian, N.

    1984-01-01

    Optical bistability and its potential for optical signal processing and communications are reviewed. Recent experimental results in semiconductor optical bistability are presented. In particular, optical bistability in GaAs-AlGaAs superlattices and CuCl, ZnS, and ZnSe is discussed.

  16. Pressure-Measuring Diaphragm Transmits Optical Signals

    NASA Technical Reports Server (NTRS)

    Hill, Arthur J.

    1990-01-01

    Proposed sapphire diaphragm performs two functions in instrumented research engine or pressure vessel. Serves both as pressure sensor and as window for observation, optical probing, or optical transmission of other measurements. Deflections of sapphire diaphragm alters resistances of strain gauges mounted around its periphery. At same time, transparent material enables optical measurements of conditions in chamber. Additional information obtained about conditions in chamber without need for additional sensor mounts.

  17. Transmission of RF Signals Over Optical Fiber for Avionics Applications

    NASA Technical Reports Server (NTRS)

    Slaveski, Filip; Sluss, James, Jr.; Atiquzzaman, Mohammed; Hung, Nguyen; Ngo, Duc

    2002-01-01

    During flight, aircraft avionics transmit and receive RF signals to/from antennas over coaxial cables. As the density and complexity of onboard avionics increases, the electromagnetic interference (EM) environment degrades proportionately, leading to decreasing signal-to-noise ratios (SNRs) and potential safety concerns. The coaxial cables are inherently lossy, limiting the RF signal bandwidth while adding considerable weight. To overcome these limitations, we have investigated a fiber optic communications link for aircraft that utilizes wavelength division multiplexing (WDM) to support the simultaneous transmission of multiple signals (including RF) over a single optical fiber. Optical fiber has many advantages over coaxial cable, particularly lower loss, greater bandwidth, and immunity to EM. In this paper, we demonstrate that WDM can be successfully used to transmit multiple RF signals over a single optical fiber with no appreciable signal degradation. We investigate the transmission of FM and AM analog modulated signals, as well as FSK digital modulated signals, over a fiber optic link (FOL) employing WDM. We present measurements of power loss, delay, SNR, carrier-to-noise ratio (CNR), total harmonic distortion (THD), and bit error rate (BER). Our experimental results indicate that WDM is a fiber optic technology suitable for avionics applications.

  18. Adaptive adjustment of reference constellation for demodulating 16QAM signal with intrinsic distortion due to imperfect modulation.

    PubMed

    Inoue, Takashi; Namiki, Shu

    2013-12-01

    We find that an adaptive equalizer and a phase-locked loop operating with decision-directed mode exhibit degraded performances when they are used in a digital coherent receiver to demodulate a 16QAM signal with intrinsically distorted constellation, and that the degradation is more significant for the dual-polarization case. We then propose a scheme to correctly demodulate such a distorted 16QAM signal, where the reference constellation and the threshold for the decision are adaptively adjusted such that they fit to the distorted ones. We experimentally confirm the improved performance of the proposed scheme over the conventional one for single-and dual-polarization 16QAM signals with distortion. We also investigate the applicable range of the proposed scheme for the degree of distortion of the signal.

  19. Optical frequency upconversion technique for transmission of wireless MIMO-type signals over optical fiber.

    PubMed

    Shaddad, R Q; Mohammad, A B; Al-Gailani, S A; Al-Hetar, A M

    2014-01-01

    The optical fiber is well adapted to pass multiple wireless signals having different carrier frequencies by using radio-over-fiber (ROF) technique. However, multiple wireless signals which have the same carrier frequency cannot propagate over a single optical fiber, such as wireless multi-input multi-output (MIMO) signals feeding multiple antennas in the fiber wireless (FiWi) system. A novel optical frequency upconversion (OFU) technique is proposed to solve this problem. In this paper, the novel OFU approach is used to transmit three wireless MIMO signals over a 20 km standard single mode fiber (SMF). The OFU technique exploits one optical source to produce multiple wavelengths by delivering it to a LiNbO3 external optical modulator. The wireless MIMO signals are then modulated by LiNbO3 optical intensity modulators separately using the generated optical carriers from the OFU process. These modulators use the optical single-sideband with carrier (OSSB+C) modulation scheme to optimize the system performance against the fiber dispersion effect. Each wireless MIMO signal is with a 2.4 GHz or 5 GHz carrier frequency, 1 Gb/s data rate, and 16-quadrature amplitude modulation (QAM). The crosstalk between the wireless MIMO signals is highly suppressed, since each wireless MIMO signal is carried on a specific optical wavelength.

  20. Optical Frequency Upconversion Technique for Transmission of Wireless MIMO-Type Signals over Optical Fiber

    PubMed Central

    Shaddad, R. Q.; Mohammad, A. B.; Al-Gailani, S. A.; Al-Hetar, A. M.

    2014-01-01

    The optical fiber is well adapted to pass multiple wireless signals having different carrier frequencies by using radio-over-fiber (ROF) technique. However, multiple wireless signals which have the same carrier frequency cannot propagate over a single optical fiber, such as wireless multi-input multi-output (MIMO) signals feeding multiple antennas in the fiber wireless (FiWi) system. A novel optical frequency upconversion (OFU) technique is proposed to solve this problem. In this paper, the novel OFU approach is used to transmit three wireless MIMO signals over a 20 km standard single mode fiber (SMF). The OFU technique exploits one optical source to produce multiple wavelengths by delivering it to a LiNbO3 external optical modulator. The wireless MIMO signals are then modulated by LiNbO3 optical intensity modulators separately using the generated optical carriers from the OFU process. These modulators use the optical single-sideband with carrier (OSSB+C) modulation scheme to optimize the system performance against the fiber dispersion effect. Each wireless MIMO signal is with a 2.4 GHz or 5 GHz carrier frequency, 1 Gb/s data rate, and 16-quadrature amplitude modulation (QAM). The crosstalk between the wireless MIMO signals is highly suppressed, since each wireless MIMO signal is carried on a specific optical wavelength. PMID:24772009

  1. PEP-19, an Intrinsically Disordered Regulator of Calmodulin Signaling*S⃞

    PubMed Central

    Kleerekoper, Quinn K.; Putkey, John A.

    2009-01-01

    PEP-19 is a small calmodulin (CaM)-binding protein that greatly increases the rates of association and dissociation of Ca2+ from the C-domain of CaM, an effect that is mediated by an acidic/IQ sequence in PEP-19. We show here using NMR that PEP-19 is an intrinsically disordered protein, but with residual structure localized to its acidic/IQ motif. We also show that the kon and koff rates for binding PEP-19 to apo-CaM are at least 50-fold slower than for binding to Ca2+-CaM. These data indicate that intrinsic disorder confers plasticity that allows PEP-19 to bind to either apo- or Ca2+-CaM via different structural modes, and that complex formation may be facilitated by conformational selection of residual structure in the acidic/IQ sequence. PMID:19106096

  2. System and method for linearly amplifying optical analog signals by backward Raman scattering

    DOEpatents

    Lin, Cheng-Heui

    1988-07-05

    A system for linearly amplifying an optical analog signal by backward stimulated Raman scattering comprises a laser source for generating a pump pulse; and an optic fiber having two opposed apertures, a first aperture for receiving the pump pulse and a second aperture for receiving the optical analog signal, wherein the optical analog signal is linearly amplified to an amplified optical analog signal.

  3. Concentric core optical fiber with multiple-mode signal transmission

    DOEpatents

    Muhs, J.D.

    1997-05-06

    A concentric core optical fiber provides for the simultaneous but independent transmission of signals over a single optical fiber. The concentric optical fiber is constructed of a single-mode or multimode inner optical fiber defined by a core and a cladding of a lower index of refraction than the core and an outer optical fiber defined by additional cladding concentrically disposed around the cladding and of an index of refraction lower than the first mentioned cladding whereby the latter functions as the core of the outer optical fiber. By employing such an optical fiber construction with a single-mode inner core or optical fiber, highly sensitive interferometric and stable less sensitive amplitude based sensors can be placed along the same length of a concentric core optical fiber. Also, by employing the concentric core optical fiber secure telecommunications can be achieved via the inner optical fiber since an intrusion of the concentric optical fiber will first cause a variation in the light being transmitted through the outer optical fiber and this variation of light being used to trigger a suitable alarm indicative of the intrusion. 3 figs.

  4. Concentric core optical fiber with multiple-mode signal transmission

    DOEpatents

    Muhs, Jeffrey D.

    1997-01-01

    A concentric core optical fiber provides for the simultaneous but independent transmission of signals over a single optical fiber. The concentric optical fiber is constructed of a single-mode or multimode inner optical fiber defined by a core and a cladding of a lower index of refraction than the core and an outer optical fiber defined by additional cladding concentrically disposed around the cladding and of an index of refraction lower than the first mentioned cladding whereby the latter functions as the core of the outer optical fiber. By employing such an optical fiber construction with a single-mode inner core or optical fiber, highly sensitive interferometric and stable less sensitive amplitude based sensors can be placed along the same length of a concentric core optical fiber. Also, by employing the concentric core optical fiber secure telecommunications can be achieved via the inner optical fiber since an intrusion of the concentric optical fiber will first cause a variation in the light being transmitted through the outer optical fiber and this variation of light being used to trigger a suitable alarm indicative of the intrusion.

  5. Optical signal acquisition and processing in future accelerator diagnostics

    SciTech Connect

    Jackson, G.P. ); Elliott, A. )

    1992-01-01

    Beam detectors such as striplines and wall current monitors rely on matched electrical networks to transmit and process beam information. Frequency bandwidth, noise immunity, reflections, and signal to noise ratio are considerations that require compromises limiting the quality of the measurement. Recent advances in fiber optics related technologies have made it possible to acquire and process beam signals in the optical domain. This paper describes recent developments in the application of these technologies to accelerator beam diagnostics. The design and construction of an optical notch filter used for a stochastic cooling system is used as an example. Conceptual ideas for future beam detectors are also presented.

  6. Optical signal acquisition and processing in future accelerator diagnostics

    SciTech Connect

    Jackson, G.P.; Elliott, A.

    1992-12-31

    Beam detectors such as striplines and wall current monitors rely on matched electrical networks to transmit and process beam information. Frequency bandwidth, noise immunity, reflections, and signal to noise ratio are considerations that require compromises limiting the quality of the measurement. Recent advances in fiber optics related technologies have made it possible to acquire and process beam signals in the optical domain. This paper describes recent developments in the application of these technologies to accelerator beam diagnostics. The design and construction of an optical notch filter used for a stochastic cooling system is used as an example. Conceptual ideas for future beam detectors are also presented.

  7. Generation of two-mode optical signals with broadband frequency tunability and low spurious signal level.

    PubMed

    Song, Ho-Jin; Shimizu, Naofumi; Nagatsuma, Tadao

    2007-10-29

    For continuous millimeter and terahertz-wave applications, a two-mode optical signal generation technique that uses two arrayed waveguide gratings and two optical switch units is presented. In addition to easy and fast operation, this scheme offers broadband frequency tunability and high signal purity with a low spurious mode level. Mode spacing, which corresponds to the frequency of the generated MM/THz-wave signal after photomixing, was successfully swept in the range of 200 ~ 550 GHz and the optical spurious mode suppression ratio higher than 25 dBc was achieved. In addition, spurious modes characteristics were investigated by using second harmonic generation (SHG) autocorrelation methods for several frequencies.

  8. Nonlinear optical polymers for electro-optic signal processing

    NASA Technical Reports Server (NTRS)

    Lindsay, Geoffrey A.

    1991-01-01

    Photonics is an emerging technology, slated for rapid growth in communications systems, sensors, imagers, and computers. Its growth is driven by the need for speed, reliability, and low cost. New nonlinear polymeric materials will be a key technology in the new wave of photonics devices. Electron-conjubated polymeric materials offer large electro-optic figures of merit, ease of processing into films and fibers, ruggedness, low cost, and a plethora of design options. Several new broad classes of second-order nonlinear optical polymers were developed at the Navy's Michelson Laboratory at China Lake, California. Polar alignment in thin film waveguides was achieved by electric-field poling and Langmuir-Blodgett processing. Our polymers have high softening temperatures and good aging properties. While most of the films can be photobleached with ultraviolet (UV) light, some have excellent stability in the 500-1600 nm range, and UV stability in the 290-310 nm range. The optical nonlinear response of these polymers is subpicosecond. Electro-optic switches, frequency doublers, light modulators, and optical data storage media are some of the device applications anticipated for these polymers.

  9. Striatal intrinsic reinforcement signals during recognition memory: relationship to response bias and dysregulation in schizophrenia.

    PubMed

    Wolf, Daniel H; Gerraty, Raphaelt; Satterthwaite, Theodore D; Loughead, James; Campellone, Timothy; Elliott, Mark A; Turetsky, Bruce I; Gur, Ruben C; Gur, Raquel E

    2011-01-01

    Ventral striatum (VS) is a critical brain region for reinforcement learning and motivation, and VS hypofunction is implicated in psychiatric disorders including schizophrenia. Providing rewards or performance feedback has been shown to activate VS. Intrinsically motivated subjects performing challenging cognitive tasks are likely to engage reinforcement circuitry even in the absence of external feedback or incentives. However, such intrinsic reinforcement responses have received little attention, have not been examined in relation to behavioral performance, and have not been evaluated for impairment in neuropsychiatric disorders such as schizophrenia. Here we used fMRI to examine a challenging "old" vs. "new" visual recognition task in healthy subjects and patients with schizophrenia. Targets were unique fractal stimuli previously presented as salient distractors in a visual oddball task, producing incidental memory encoding. Based on the prediction error theory of reinforcement learning, we hypothesized that correct target recognition would activate VS in controls, and that this activation would be greater in subjects with lower expectation of responding correctly as indexed by a more conservative response bias. We also predicted these effects would be reduced in patients with schizophrenia. Consistent with these predictions, controls activated VS and other reinforcement processing regions during correct recognition, with greater VS activation in those with a more conservative response bias. Patients did not show either effect, with significant group differences suggesting hyporesponsivity in patients to internally generated feedback. These findings highlight the importance of accounting for intrinsic motivation and reward when studying cognitive tasks, and add to growing evidence of reward circuit dysfunction in schizophrenia that may impact cognition and function.

  10. Method for enhancing signals transmitted over optical fibers

    DOEpatents

    Ogle, J.W.; Lyons, P.B.

    1981-02-11

    A method for spectral equalization of high frequency spectrally broadband signals transmitted through an optical fiber is disclosed. The broadband signal input is first dispersed by a grating. Narrow spectral components are collected into an array of equalizing fibers. The fibers serve as optical delay lines compensating for material dispersion of each spectral component during transmission. The relative lengths of the individual equalizing fibers are selected to compensate for such prior dispersion. The output of the equalizing fibers couple the spectrally equalized light onto a suitable detector for subsequent electronic processing of the enhanced broadband signal.

  11. Variable-time-delay optical coherent transient signal processing.

    PubMed

    Merkel, K D; Babbitt, W R; Anderson, K E; Wagner, K H

    1999-10-15

    A technique is proposed and experimentally demonstrated that achieves simultaneous optical pattern waveform storage and programmable time delay for continuous real-time signal processing by use of optical coherent transient technology. We achieve variable-time-delay and broadband signal processing by frequency shifting of two chirped programming pulses, the chirp rate of one being twice that of the other, without using brief reference pulses and without changing the timing of the programming sequence. We demonstrate the technique experimentally in Tm(3+): YAG at 5 K for 40-MHz chirps by performing temporal signal convolution with true-time delays that vary over a 250-ns range.

  12. Method for enhancing signals transmitted over optical fibers

    DOEpatents

    Ogle, James W.; Lyons, Peter B.

    1983-01-01

    A method for spectral equalization of high frequency spectrally broadband signals transmitted through an optical fiber. The broadband signal input is first dispersed by a grating. Narrow spectral components are collected into an array of equalizing fibers. The fibers serve as optical delay lines compensating for material dispersion of each spectral component during transmission. The relative lengths of the individual equalizing fibers are selected to compensate for such prior dispersion. The output of the equalizing fibers couple the spectrally equalized light onto a suitable detector for subsequent electronic processing of the enhanced broadband signal.

  13. Nonlinear fiber applications for ultrafast all-optical signal processing

    NASA Astrophysics Data System (ADS)

    Kravtsov, Konstantin

    In the present dissertation different aspects of all-optical signal processing, enabled by the use of nonlinear fibers, are studied. In particular, we focus on applications of a novel heavily GeO2-doped (HD) nonlinear fiber, that appears to be superior to many other types of nonlinear fibers because of its high nonlinearity and suitability for the use in nonlinear optical loop mirrors (NOLMs). Different functions, such as all-optical switching, thresholding, and wavelength conversion, are demonstrated with the HD fibers in the NOLM configuration. These basic functions are later used for realization of ultrafast time-domain demultiplexers, clock recovery, detectors of short pulses in stealth communications, and primitive elements for analog computations. Another important technology that benefits from the use of nonlinear fiber-based signal processing is optical code-division multiple access (CDMA). It is shown in both theory and experiment that all-optical thresholding is a unique way of improving existing detection methods for optical CDMA. Also, it is the way of implementation of true asynchronous optical spread-spectrum networks, which allows full realization of optical CDMA potential. Some aspects of quantum signal processing and manipulation of quantum states are also studied in this work. It is shown that propagation and collisions of Thirring solitons lead to a substantial squeezing of quantum states, which may find applications for generation of squeezed light.

  14. B cell-intrinsic MyD88 signaling prevents the lethal dissemination of commensal bacteria during colonic damage

    PubMed Central

    Kirkland, Donna; Benson, Alicia; Mirpuri, Julie; Pifer, Reed; Hou, Baidong; DeFranco, Anthony L.; Yarovinsky, Felix

    2012-01-01

    Summary The Toll-like receptor adaptor protein MyD88 is essential for the regulation of intestinal homeostasis in mammals. In this study, we determined that Myd88-deficient mice are susceptible to colonic damage that is induced by dextran sulfate sodium (DSS) administration due to uncontrolled dissemination of intestinal commensal bacteria. The DSS-induced mortality of Myd88-deficient mice was completely prevented by antibiotic treatment to deplete commensal bacteria. By using cell type-specific Myd88-deficient mice, we established that B cell-intrinsic MyD88 signaling plays a central role in the resistance to DSS-induced colonic damage via the production of IgM and complement-mediated control of intestinal bacteria. Our results indicate that the lack of intact MyD88 signaling in B cells, coupled with impaired epithelial integrity, enables commensal bacteria to function as highly pathogenic organisms, causing rapid host death. PMID:22306056

  15. Down-Regulation of AKT Signalling by Ursolic Acid Induces Intrinsic Apoptosis and Sensitization to Doxorubicin in Soft Tissue Sarcoma

    PubMed Central

    Villar, Victor Hugo; Vögler, Oliver; Barceló, Francisca; Martín-Broto, Javier; Martínez-Serra, Jordi; Ruiz-Gutiérrez, Valentina; Alemany, Regina

    2016-01-01

    Several important biological activities have been attributed to the pentacyclic triterpene ursolic acid (UA), being its antitumoral effect extensively studied in human adenocarcinomas. In this work, we focused on the efficacy and molecular mechanisms involved in the antitumoral effects of UA, as single agent or combined with doxorubicin (DXR), in human soft tissue sarcoma cells. UA (5–50 μM) strongly inhibited (up to 80%) the viability of STS cells at 24 h and its proliferation in soft agar, with higher concentrations increasing apoptotic death up to 30%. UA treatment (6–9 h) strongly blocked the survival AKT/GSK3β/β-catenin signalling pathway, which led to a concomitant reduction of the anti-apoptotic proteins c-Myc and p21, altogether resulting in the activation of intrinsic apoptosis. Interestingly, UA at low concentrations (10–15 μM) enhanced the antitumoral effects of DXR by up to 2-fold, while in parallel inhibiting DXR-induced AKT activation and p21 expression, two proteins implicated in antitumoral drug resistance and cell survival. In conclusion, UA is able to induce intrinsic apoptosis in human STS cells and also to sensitize these cells to DXR by blocking the AKT signalling pathway. Therefore, UA may have beneficial effects, if used as nutraceutical adjuvant during standard chemotherapy treatment of STS. PMID:27219337

  16. Hybrid integrated optic modules for real-time signal processing

    NASA Technical Reports Server (NTRS)

    Tsai, C. S.

    1984-01-01

    The most recent progress on four relatively new hybrid integrated optic device modules in LiNbO3 waveguides and one in YIG/GGG waveguide that are currently being studied are discussed. The five hybrid modules include a time-integrating acoustooptic correlator, a channel waveguide acoustooptic frequency shifter/modulator, an electrooptic channel waveguide total internal reflection moculator/switch, an electrooptic analog-to-digital converter using a Fabry-Perot modulator array, and a noncollinear magnetooptic modulator using magnetostatic surface waves. All of these devices possess the desirable characteristics of very large bandwidth (GHz or higher), very small substrate size along the optical path (typically 1.5 cm or less), single-mode optical propagation, and low drive power requirement. The devices utilize either acoustooptic, electrooptic or magnetooptic effects in planar or channel waveguides and, therefore, act as efficient interface devices between a light wave and temporal signals. Major areas of application lie in wideband multichannel optical real-time signal processing and communications. Some of the specific applications include spectral analysis and correlation of radio frequency (RF) signals, fiber-optic sensing, optical computing and multiport switching/routing, and analog-to-digital conversion of wide RF signals.

  17. Clock recovery from 40 Gbps optical signal with optical phase-locked loop based on a terahertz optical asymmetric demultiplexer

    NASA Astrophysics Data System (ADS)

    Jhon, Young Min; Ki, Ho Jin; Kim, Sun Ho

    2003-05-01

    10 GHz clock recovery from 40 Gbps optical time-division-multiplexed (OTDM) signal pulses is experimentally demonstrated using optical phase lock loop based on a terahertz optical asymmetric demultiplexer (TOAD) with a local-reference-oscillator-free electronic feedback circuit. The clock pulse that was used as the control pulse had energy of 800 fJ and the SNR of the time-extracted 10 GHz RF signal to the side components was larger than 40 dB.

  18. The Potential of Brittle Star Extracted Polysaccharide in Promoting Apoptosis via Intrinsic Signaling Pathway

    PubMed Central

    Baharara, Javad; Amini, Elaheh

    2015-01-01

    Background: Anti-cancer potential of marine natural products such as polysaccharides represented therapeutic potential in oncological researches. In this study, total polysaccharide from brittle star [Ophiocoma erinaceus (O. erinaceus)] was extracted and chemopreventive efficacy of Persian Gulf brittle star polysaccharide was investigated in HeLa human cervical cancer cells. Methods: To extract polysaccharide, dried brittle stars were ground and extracted mechanically. Then, detection of polysaccharide was performed by phenol sulfuric acid, Ultra Violet (UV)-sulfuric acid method and FTIR. The anti proliferative activity of isolated polysaccharide was examined by MTT assay and evaluation of cell death was done through morphological cell changes; Propodium Iodide staining, fluorescence microscopy and caspase-3, -9 enzymatic measurements. To assess its underlying mechanism, expression of Bax, Bcl-2 was evaluated. Results: The polysaccharide detection methods demonstrated isolation of crude polysaccharide from Persian Gulf brittle star. The results revealed that O. erinaceus polysaccharide suppressed the proliferation of HeLa cells in a dose and time dependent manner. Morphological observation of DAPI and Acridine Orange/Propodium Iodide staining was documented by typical characteristics of apoptotic cell death. Flow cytometry analyses exhibited the accumulation of treated cells in sub-G1 region. Additionally, polysaccharide extracted induced intrinsic apoptosis via up-regulation of caspase-3, caspase-9 and Bax along with down-regulation of Bcl-2 in HeLa cells. Conclusion: Taken together, the apoptosis inducing effect of brittle star polysaccharide via intrinsic pathway confirmed the anti tumor potential of marine polysaccharide. Therefore, these findings proposed new insight into anti cancer properties of brittle star polysaccharide as a promising agent in cervical cancer treatment. PMID:26605009

  19. Demonstration of optical steganography transmission using temporal phase coded optical signals with spectral notch filtering.

    PubMed

    Hong, Xuezhi; Wang, Dawei; Xu, Lei; He, Sailing

    2010-06-01

    A novel approach is proposed and experimentally demonstrated for optical steganography transmission in WDM networks using temporal phase coded optical signals with spectral notch filtering. A temporal phase coded stealth channel is temporally and spectrally overlaid onto a public WDM channel. Direct detection of the public channel is achieved in the presence of the stealth channel. The interference from the public channel is suppressed by spectral notching before the detection of the optical stealth signal. The approach is shown to have good compatibility and robustness to the existing WDM network for optical steganography transmission.

  20. Nonlinear optical signal processing for high-speed, spectrally efficient fiber optic systems and networks

    NASA Astrophysics Data System (ADS)

    Zhang, Bo

    The past decade has witnessed astounding boom in telecommunication network traffic. With the emergence of multimedia over Internet, the high-capacity optical transport systems have started to shift focus from the core network towards the end users. This trend leads to diverse optical networks with transparency and reconfigurability requirement. As single channel data rate continues to increase and channel spacing continues to shrink for high capacity, high spectral efficiency, the workload on conventional electronic signal processing elements in the router nodes continues to build up. Performing signal processing functions in the optical domain can potentially alleviate the speed bottleneck if the unique optical properties are efficiently leveraged to assist electronic processing methodologies. Ultra-high bandwidth capability along with the promise for multi-channel and format-transparent operation make optical signal processing an attractive technology which is expected to have great impact on future optical networks. For optical signal processing applications in fiber-optic network and systems, a laudable goal would be to explore the unique nonlinear optical processes in novel photonic devices. This dissertation investigates novel optical signal processing techniques through simulations and experimental demonstrations, analyzes limitations of these nonlinear processing elements and proposes techniques to enhance the system performance or designs for functional photonic modules. Two key signal-processing building blocks for future optical networks, namely slow-light-based tunable optical delay lines and SOA-based high-speed wavelength converters, are presented in the first part of the dissertation. Phase preserving and spectrally efficient slow light are experimentally demonstrated using advanced modulation formats. Functional and novel photonic modules, such as multi-channel synchronizer and variable-bit-rate optical time division multiplexer are designed and

  1. Interventional nerve visualization via the intrinsic anisotropic optical properties of the nerves

    NASA Astrophysics Data System (ADS)

    Chin, Kenneth W.; Meijerink, Andries; Chin, Patrick T. K.

    2015-07-01

    We present an optical concept to visualize nerves during surgical interventions. The concept relies on the anisotropic optical properties of the nerves which allows for specific switching of the optical reflection by the nervous tissue. Using a low magnification polarized imaging system we are able to visualize the on and off switching of the optical reflection of the nervous tissue, enabling a non-invasive nerve specific real-time nerve visualization during surgery.

  2. Signal restoration in intensity-modulated optical OFDM access systems.

    PubMed

    Vanin, Evgeny

    2011-11-15

    It is well known that deliberate signal clipping in an intensity-modulated (IM) laser transmitter helps to overcome the optical orthogonal frequency division multiplexing (OFDM) system performance limitation that is related to the signal high peak-to-average power ratio. The amplitude of a clipped OFDM signal has to be optimized in order to minimize the optical power that is required to achieve a specified system performance. However, the signal clipping introduces nonlinear distortion (so-called clipping noise) and leads to a system performance penalty. In this Letter, the performance of the IM optical OFDM system with digital baseband clipping distortion in the transmitter and clipping noise compensation by means of signal restoration in the digital signal processing unit of the system receiver is analytically evaluated. It is demonstrated that the system bit-error ratio can be reduced by more than an order of magnitude, from 10(-3) to 3.5×10(-5), by applying only the first iteration of the signal restoration algorithm proposed in this Letter. The results of the analytical analysis are verified with brute-force numerical simulations based on direct error counting. PMID:22089556

  3. Silicon technology compatible photonic molecules for compact optical signal processing

    SciTech Connect

    Barea, Luis A. M. Vallini, Felipe; Jarschel, Paulo F.; Frateschi, Newton C.

    2013-11-11

    Photonic molecules (PMs) based on multiple inner coupled microring resonators allow to surpass the fundamental constraint between the total quality factor (Q{sub T}), free spectral range (FSR), and resonator size. In this work, we use a PM that presents doublets and triplets resonance splitting, all with high Q{sub T}. We demonstrate the use of the doublet splitting for 34.2 GHz signal extraction by filtering the sidebands of a modulated optical signal. We also demonstrate that very compact optical modulators operating 2.75 times beyond its resonator linewidth limit may be obtained using the PM triplet splitting, with separation of ∼55 GHz.

  4. Activation of intrinsic apoptotic signaling pathway in cancer cells by Cymbopogon citratus polysaccharide fractions.

    PubMed

    Thangam, Ramar; Sathuvan, Malairaj; Poongodi, Arasu; Suresh, Veeraperumal; Pazhanichamy, Kalailingam; Sivasubramanian, Srinivasan; Kanipandian, Nagarajan; Ganesan, Nalini; Rengasamy, Ramasamy; Thirumurugan, Ramasamy; Kannan, Soundarapandian

    2014-07-17

    Essential oils of Cymbopogon citratus were already reported to have wide ranging medical and industrial applications. However, information on polysaccharides from the plant and their anticancer activities are limited. In the present study, polysaccharides from C. citratus were extracted and fractionated by anion exchange and gel filtration chromatography. Two different polysaccharide fractions such as F1 and F2 were obtained, and these fractions were found to have distinct acidic polysaccharides as characterized by their molecular weight and sugar content. NMR spectral analysis revealed the presence of (1→4) linked b-d-Xylofuranose moiety in these polysaccharides. Using these polysaccharide fractions F1 and F2, anti-inflammatory and anticancer activities were evaluated against cancer cells in vitro and the mechanism of action of the polysaccharides in inducing apoptosis in cancer cells via intrinsic pathway was also proposed. Two different reproductive cancer cells such as Siha and LNCap were employed for in vitro studies on cytotoxicity, induction of apoptosis and apoptotic DNA fragmentation, changes in mitochondrial membrane potential, and profiles of gene and protein expression in response to treatment of cells by the polysaccharide fractions. These polysaccharide fractions exhibited potential cytotoxic and apoptotic effects on carcinoma cells, and they induced apoptosis in these cells through the events of up-regulation of caspase 3, down-regulation of bcl-2 family genes followed by cytochrome c release.

  5. Activation of cell signaling via optical manipulation of gold-coated liposomes encapsulating signaling molecules

    NASA Astrophysics Data System (ADS)

    Orsinger, Gabriel V.; Leung, Sarah J.; Romanowski, Marek

    2013-02-01

    Many diseases involve changes in cell signaling cascades, as seen commonly in drug resistant cancers. To better understand these intricate signaling events in diseased cells and tissues, experimental methods of probing cellular communication at a single to multi-cell level are required. We recently introduced a general platform for activation of selected signaling pathways by optically controlled delivery and release of water soluble factors using gold-coated liposomes. In the example presented here, we encapsulated inositol trisphosphate (IP3), a ubiquitous intracellular secondary messenger involved in GPCR and Akt signaling cascades, within 100 nm gold-coated liposomes. The high polarizability of the liposome's unique gold pseudo-shell allows stable optical trapping for subcellular manipulation in the presence of cells. We take this optical manipulation further by optically injecting IP3-containing liposomes into the cytosol of a single cell to initiate localized cell signaling. Upon optical injection of liposomal IP3 into a single ovarian carcinoma cell, we observed localized activation as reported by changes in Indo-1 fluorescence intensity. With established gap junctions between the injected cell and neighboring cells, we monitored propagation of this signaling to and through nearby cells.

  6. Optical generation of millimeter-wave signals via frequency 16-tupling without an optical filter

    NASA Astrophysics Data System (ADS)

    Zhu, Zihang; Zhao, Shanghong; Chu, Xingchun; Dong, Yi

    2015-11-01

    Microwave frequency multiplication using two cascaded dual-parallel Mach-Zehnder modulators (DP-MZMs) to generate an optical millimeter-wave (mm-wave) with 16-tupling of the radio frequency (RF) driving signal frequency is proposed. A theoretical analysis leading to the optimum operating conditions including the direct current (dc) bias points, the modulation index, and the phase difference between RF driving signals is developed and demonstrated. A 160 GHz mm-wave with an optical sideband suppression ratio (OSSR) of 21.5 dB and a radio frequency spurious suppression ratio (RFSSR) of 38 dB is generated from a 10 GHz RF driving signal, which largely reduce the frequency of electrical driving signal and electrooptic devices. Since no optical filter is employed to suppress the undesired optical sidebands, the system exhibits the advantage of large frequency tunable range.

  7. Optically Modulated Fluorophores for Selective Fluorescence Signal Recovery

    PubMed Central

    Richards, Chris I.; Hsiang, Jung-Cheng; Senapati, Dulal; Patel, Sandeep; Yu, Junhua; Vosch, Tom; Dickson, Robert M.

    2009-01-01

    Fluorescence imaging in biological sciences is hindered by significant depth-dependent signal attenuation and high fluorescent backgrounds. We have developed optically modulated near-IR-emitting few-atom Ag nanodots that are selectively and dynamically photobrightened upon simultaneous excitation with a secondary laser enabling high sensitivity image extraction to reveal only the demodulated fluorophores. Image demodulation is demonstrated in high background environments to extract weak signals from completely obscuring background emission. PMID:19284790

  8. Digital signal processing for fiber-optic thermometers

    SciTech Connect

    Fernicola, V.; Crovini, L.

    1994-12-31

    A digital signal processing scheme for measurement of exponentially-decaying signals, such as those found in fluorescence, lifetime-based, fiber-optic sensors, is proposed. The instrument uses a modified digital phase-sensitive-detection technique with the phase locked to a fixed value and the modulation period tracking the measured lifetime. Typical resolution of the system is 0.05% for slow decay (>500 {mu}s) and 0.1% for fast decay.

  9. Reversible Gating of Plasmonic Coupling for Optical Signal Amplification.

    PubMed

    Khoury, Christopher G; Fales, Andrew M; Vo-Dinh, Tuan

    2016-07-20

    Amplification of optical signals is useful for a wide variety of applications, ranging from data signal transmission to chemical sensing and biomedical diagnostics. One such application in chemical sensing is surface-enhanced Raman scattering (SERS), an important technique for increasing the Raman signal using the plasmonic effect of enhanced electromagnetic fields associated with metallic nanostructures. One of the most important limitations of SERS-based amplification is the difficulty to reproducibly control the SERS signal. Here, we describe the design and implementation of a unique hybrid system capable of producing reversible gating of plasmonic coupling for Raman signal amplification. The hybrid system is composed of two subsystems: (1) colloidal magneto-plasmonic nanoparticles for SERS enhancement and (2) a micromagnet substrate with an externally applied magnetic field to modulate the colloidal nanoparticles. For this proof of concept demonstration, the nanoparticles were labeled with a Raman-active dye, and it was shown that the detected SERS signal could be reproducibly modulated by controlling the externally applied magnetic field. The developed system provides a simple, robust, inexpensive, and reusable device for SERS signal modulation. These properties will open up new possibilities for optical signal amplification and gating as well for high-throughput, reproducible SERS detection.

  10. Signal Readout Using Small Near-Field Optical Head with Horizontal Light Introduction Through Optical Fiber

    NASA Astrophysics Data System (ADS)

    Kato, Kenji; Ichihara, Susumu; Oumi, Manabu; Maeda, Hidetaka; Niwa, Takashi; Mitsuoka, Yasuyuki; Nakajima, Kunio; Ohkubo, Toshifumi; Itao, Kiyoshi

    2003-08-01

    We have developed a small near-field optical head for high-recording-density data storage applications, to overcome the disadvantage of conventional near-field optical heads, such as large light introduction, low optical throughput, and difficulty in controlling the aperture-medium distance. The optical head structure has miniaturized light introduction using an optical fiber placed horizontally. To decrease the optical loss, an integrated microlens focuses on the aperture tip that has a shortened cut-off region. The fabricated optical head (3.2× 3.6× 0.9 mm3) with a φ 200 nm aperture of the same height as the sliders shows a clear readout signal of a 200-nm-wide line and space pattern at a speed of 5.2 MHz. We show that the optical head has the ability to read 4 times the recording density by simulations of the finite difference time domain (FDTD).

  11. BPSK optical mm-wave signal generation by septupling frequency via a single optical phase modulator

    NASA Astrophysics Data System (ADS)

    Wu, Peng; Ma, Jianxin

    2016-09-01

    In this paper, we have proposed a novel and simple scheme to generate the BPSK optical millimeter wave (MMW) signal with frequency septupling by using an optical phase modulator (PM) and a wavelength selective switch (WSS). In this scheme, the PM is driven by a radio frequency (RF) BPSK signal at the optimized modulation index of 4.89 to assure the 4th and 3rd-order sidebands have equal amplitudes. An wavelength selective switch (WSS) is used to abstract the -4th and +3rd-order sidebands from the spectrum generated by RF BPSK signal modulating the lightwave to form the BPSK optical MMW signal with frequency septupling the driving RF signal. In these two tones, only the +3rd-order sideband bears the BPSK signal while the -4th-order sideband is unmodulated since the phase information is canceled by the even times multiplication of the phase of BPSK signal. The MMW signal can avoid the pulse walk-off effect and the amplitude fading effect caused by the fiber chromatic dispersion. By adjusting the modulation index to assure the two tones have equal amplitude, the generated optical MMW signal has the maximal opto-electrical conversion efficiency and good transmission performance.

  12. Visible light communications using predistortion signal to enhance the response of passive optical receiver

    NASA Astrophysics Data System (ADS)

    Liu, Yang; Chen, Hung-Yu; Liang, Kevin; Wei, Liang-Yu; Chow, Chi-Wai; Yeh, Chien-Hung

    2016-01-01

    Traditional visible light communication (VLC) uses positive-intrinsic-negative photodiode (PD) or avalanche PD as the optical receivers (Rx). We demonstrate using a solar cell as the VLC Rx. The solar cell is flexible and low cost and converts the optical signal into an electrical signal directly without the need of external power supply. In addition to acting as the VLC passive Rx, the converted electrical signal from the solar cell can charge up the battery of the Rx nodes. Hence, the proposed scheme can be a promising candidate for the future Internet of Things network. However, a solar cell acting as a VLC Rx is very challenging, since the response of the solar cell is limited. Here, we propose and demonstrate using predistortion to significantly enhance the solar cell Rx response for the first time up to the authors' knowledge. Experimental results show that the response of the solar cell Rx is significantly enhanced; and the original 2-kHz detection bandwidth of the solar cell can be enhanced by 250 times for receiving 500-kbit/s VLC signal at a transmission distance of 1 m. The operation principle, the generated voltage by the solar cell, and the maximum data rates achieved at different transmission distances are also studied.

  13. Optical signal processing using photonic reservoir computing

    NASA Astrophysics Data System (ADS)

    Salehi, Mohammad Reza; Dehyadegari, Louiza

    2014-10-01

    As a new approach to recognition and classification problems, photonic reservoir computing has such advantages as parallel information processing, power efficient and high speed. In this paper, a photonic structure has been proposed for reservoir computing which is investigated using a simple, yet, non-partial noisy time series prediction task. This study includes the application of a suitable topology with self-feedbacks in a network of SOA's - which lends the system a strong memory - and leads to adjusting adequate parameters resulting in perfect recognition accuracy (100%) for noise-free time series, which shows a 3% improvement over previous results. For the classification of noisy time series, the rate of accuracy showed a 4% increase and amounted to 96%. Furthermore, an analytical approach was suggested to solve rate equations which led to a substantial decrease in the simulation time, which is an important parameter in classification of large signals such as speech recognition, and better results came up compared with previous works.

  14. Observation of intrinsic size effects in the optical response of individual gold nanoparticles.

    PubMed

    Berciaud, Stéphane; Cognet, Laurent; Tamarat, Philippe; Lounis, Brahim

    2005-03-01

    The photothermal heterodyne imaging method is used to study for the first time the absorption spectra of individual gold nanoparticles with diameters down to 5 nm. Intrinsic size effects that result in a broadening of the surface plasmon resonance are unambiguously observed. Dispersions in the peak energies and homogeneous widths of the single-particle resonances are revealed. The experimental results are analyzed within the frame of Mie theory. PMID:15755105

  15. Intrinsic dorsoventral patterning and extrinsic EGFR signaling genes control glial cell development in the Drosophila nervous system.

    PubMed

    Kim, H J; Ahn, H J; Lee, S; Kim, J H; Park, J; Jeon, S-H; Kim, S H

    2015-10-29

    Dorsoventral patterning and epidermal growth factor receptor (EGFR) signaling genes are essential for determining neural identity and differentiation of the Drosophila nervous system. Their role in glial cell development in the Drosophila nervous system is not clearly established. Our study demonstrated that the dorsoventral patterning genes, vnd, ind, and msh, are intrinsically essential for the proper expression of a master glial cell regulator, gcm, and a differentiation gene, repo, in the lateral glia. In addition, we showed that esg is particularly required for their expression in the peripheral glia. These results indicate that the dorsoventral patterning and EGFR signaling genes are essential for identity determination and differentiation of the lateral glia by regulating proper expression of gcm and repo in the lateral glia from the early glial development. In contrast, overexpression of vnd, msh, spi, and Egfr genes repressed the expression of Repo in the ventral neuroectoderm, indicating that maintenance of correct columnar identity along the dorsoventral axis by proper expression of these genes is essential for restrictive formation of glial precursor cells in the lateral neuroectoderm. Therefore, the dorsoventral patterning and EGFR signaling genes play essential roles in correct identity determination and differentiation of lateral glia in the Drosophila nervous system.

  16. Intrinsic dorsoventral patterning and extrinsic EGFR signaling genes control glial cell development in the Drosophila nervous system.

    PubMed

    Kim, H J; Ahn, H J; Lee, S; Kim, J H; Park, J; Jeon, S-H; Kim, S H

    2015-10-29

    Dorsoventral patterning and epidermal growth factor receptor (EGFR) signaling genes are essential for determining neural identity and differentiation of the Drosophila nervous system. Their role in glial cell development in the Drosophila nervous system is not clearly established. Our study demonstrated that the dorsoventral patterning genes, vnd, ind, and msh, are intrinsically essential for the proper expression of a master glial cell regulator, gcm, and a differentiation gene, repo, in the lateral glia. In addition, we showed that esg is particularly required for their expression in the peripheral glia. These results indicate that the dorsoventral patterning and EGFR signaling genes are essential for identity determination and differentiation of the lateral glia by regulating proper expression of gcm and repo in the lateral glia from the early glial development. In contrast, overexpression of vnd, msh, spi, and Egfr genes repressed the expression of Repo in the ventral neuroectoderm, indicating that maintenance of correct columnar identity along the dorsoventral axis by proper expression of these genes is essential for restrictive formation of glial precursor cells in the lateral neuroectoderm. Therefore, the dorsoventral patterning and EGFR signaling genes play essential roles in correct identity determination and differentiation of lateral glia in the Drosophila nervous system. PMID:26318336

  17. Intrinsic proinflammatory signaling in podocytes contributes to podocyte damage and prolonged proteinuria.

    PubMed

    Brähler, Sebastian; Ising, Christina; Hagmann, Henning; Rasmus, Melanie; Hoehne, Martin; Kurschat, Christine; Kisner, Tuelay; Goebel, Heike; Shankland, Stuart; Addicks, Klaus; Thaiss, Friedrich; Schermer, Bernhard; Pasparakis, Manolis; Benzing, Thomas; Brinkkoetter, Paul Thomas

    2012-11-15

    Inflammation conveys the development of glomerular injury and is a major cause of progressive kidney disease. NF-κB signaling is among the most important regulators of proinflammatory signaling. Its role in podocytes, the epithelial cells at the kidney filtration barrier, is poorly understood. Here, we inhibited NF-κB signaling in podocytes by specific ablation of the NF-κB essential modulator (NEMO, IKKγ). Podocyte-specific NEMO-deficient mice (NEMO(pko)) were viable and did not show proteinuria or overt changes in kidney morphology. After induction of glomerulonephritis, both NEMO(pko) and control mice developed significant proteinuria. However, NEMO(pko) mice recovered much faster, showing rapid remission of proteinuria and restoration of podocyte morphology. Interestingly, quantification of infiltrating macrophages, T-lymphocytes, and granulocytes at day 7 revealed no significant difference between wild-type and NEMO(pko). To further investigate the underlying mechanisms, we created a stable NEMO knockdown mouse podocyte cell line. Again, no overt changes in morphology were observed. Translocation of NF-κB to the nucleus after stimulation with TNFα or IL-1 was sufficiently inhibited. Moreover, secretion of proinflammatory chemokines from podocytes after stimulation with TNFα or IL-1 was significantly reduced in NEMO-deficient podocytes and in glomerular samples obtained at day 7 after induction of nephrotoxic nephritis. Collectively, these results show that proinflammatory activity of NF-κB in podocytes aggravates proteinuria in experimental glomerulonephritis in mice. Based on these data, it may be speculated that immunosuppressive drugs may not only target professional immune cells but also podocytes directly to convey their beneficial effects in various types of glomerulonephritis.

  18. Direct Ubiquitin Independent Recognition and Degradation of a Folded Protein by the Eukaryotic Proteasomes-Origin of Intrinsic Degradation Signals

    PubMed Central

    Singh Gautam, Amit Kumar; Balakrishnan, Satish; Venkatraman, Prasanna

    2012-01-01

    Eukaryotic 26S proteasomes are structurally organized to recognize, unfold and degrade globular proteins. However, all existing model substrates of the 26S proteasome in addition to ubiquitin or adaptor proteins require unstructured regions in the form of fusion tags for efficient degradation. We report for the first time that purified 26S proteasome can directly recognize and degrade apomyoglobin, a globular protein, in the absence of ubiquitin, extrinsic degradation tags or adaptor proteins. Despite a high affinity interaction, absence of a ligand and presence of only helices/loops that follow the degradation signal, apomyoglobin is degraded slowly by the proteasome. A short floppy F-helix exposed upon ligand removal and in conformational equilibrium with a disordered structure is mandatory for recognition and initiation of degradation. Holomyoglobin, in which the helix is buried, is neither recognized nor degraded. Exposure of the floppy F-helix seems to sensitize the proteasome and primes the substrate for degradation. Using peptide panning and competition experiments we speculate that initial encounters through the floppy helix and additional strong interactions with N-terminal helices anchors apomyoglobin to the proteasome. Stabilizing helical structure in the floppy F-helix slows down degradation. Destabilization of adjacent helices accelerates degradation. Unfolding seems to follow the mechanism of helix unraveling rather than global unfolding. Our findings while confirming the requirement for unstructured regions in degradation offers the following new insights: a) origin and identification of an intrinsic degradation signal in the substrate, b) identification of sequences in the native substrate that are likely to be responsible for direct interactions with the proteasome, and c) identification of critical rate limiting steps like exposure of the intrinsic degron and destabilization of an unfolding intermediate that are presumably catalyzed by the ATPases

  19. Improvement of the signal integrity in diffractive optical encoders

    NASA Astrophysics Data System (ADS)

    Kress, Bernard; Mueller, Ulrich

    2014-09-01

    We are presenting several techniques to improve the quality of the signals in diffractive optics encoders, for either linear and rotational encoders. We have developed previously various hybrid incremental/absolute disk based rotary diffractive encoders architectures. While the binary signals for absolute encoding were usually of sufficiently good quality to retrieve the entire Gray code signal over the desired resolutions (10, 12 or 14 bits), the quality and integrity of the sinusoidal signals for the incremental part of the encoder needed to be improved, since these are the signals allowing the encoder to go to much higher interpolated resolutions (20 bits or over). A good precision over the interpolated signals assumes very accurate sinusoidal profiles form the raw signals. Strong interpolation can only be done on high quality sinusoidal native signals (also referred to as pulses per revolution or PPR). A typical high resolution incremental encoder might provide 12 to 16 native sinusoidal PPRs, but the interpolation over these signals can reach way over 20 bits of resolution if the signals are of good quality.

  20. Jak/Stat Signaling Stimulates Zebrafish Optic Nerve Regeneration and Overcomes the Inhibitory Actions of Socs3 and Sfpq

    PubMed Central

    Elsaeidi, Fairouz; Bemben, Michael A.; Zhao, Xiao-Feng

    2014-01-01

    The regenerative failure of mammalian optic axons is partly mediated by Socs3-dependent inhibition of Jak/Stat signaling (Smith et al., 2009, 2011). Whether Jak/Stat signaling is part of the normal regenerative response observed in animals that exhibit an intrinsic capacity for optic nerve regeneration, such as zebrafish, remains unknown. Nor is it known whether the repression of regenerative inhibitors, such as Socs3, contributes to the robust regenerative response of zebrafish to optic nerve damage. Here we report that Jak/Stat signaling stimulates optic nerve regeneration in zebrafish. We found that IL-6 family cytokines, acting via Gp130-coupled receptors, stimulate Jak/Stat3 signaling in retinal ganglion cells after optic nerve injury. Among these cytokines, we found that CNTF, IL-11, and Clcf1/Crlf1a can stimulate optic axon regrowth. Surprisingly, optic nerve injury stimulated the expression of Socs3 and Sfpq (splicing factor, proline/glutamine rich) that attenuate optic nerve regeneration. These proteins were induced in a Jak/Stat-dependent manner, stimulated each other's expression and suppressed the expression of regeneration-associated genes. In vivo, the injury-dependent induction of Socs3 and Sfpq inhibits optic nerve regeneration but does not block it. We identified a robust induction of multiple cytokine genes in zebrafish retinal ganglion cells that may contribute to their ability to overcome these inhibitory factors. These studies not only identified mechanisms underlying optic nerve regeneration in fish but also suggest new molecular targets for enhancing optic nerve regeneration in mammals. PMID:24523552

  1. Signaling for fast restoration in heterogeneous optical mesh networks

    NASA Astrophysics Data System (ADS)

    Rajagopalan, Bala; Saha, Debanjan; Bernstein, Greg; Sharma, Vishal

    2001-10-01

    With the advent of optical mesh networks, certain new protection schemes have been defined. This encompasses both local span and end-to-end path protection. But the implementations of these protection schemes have so far been based on proprietary mechanisms developed by each vendor. This has made it virtually impractical to construct a heterogeneous network with interoperable mesh protection schemes. Also, while the notion of a standard IP-centric control plane for optical networks based on Generalized Multi-Protocol Label Switching (GMPLS) has gained wide acceptance, the work in this area has so far focused exclusively on connection provisioning rather than restoration. This paper defines standard, IP-based signaling protocols for restoration in optical mesh networks. These protocols focus on a new local span protection mode and end-to-end shared protection. The main requirements on these protocols are simplicity and speed. The signaling mechanisms described in this paper are complimentary to the GMPLS provisioning mechanisms.

  2. Multiplexed interferometric fiber-optic sensors with digital signal processing.

    PubMed

    Sadkowski, R; Lee, C E; Taylor, H F

    1995-09-01

    A microcontroller-based digital signal processing system developed for use with fiber-optic sensors for measuring pressure in internal combustion engines is described. A single distributed feedback laser source provides optical power for four interferometric sensors. The laser current is repetitively modulated so that its optical frequency is nearly a linear function of time over most of a cycle. The interferometer phase shift is proportional to the elapsed time from the initiation of a sawtooth until the sensor output signal level crosses a threshold value proportional to the laser output power. This elapsed time, assumed to vary linearly with the combustion chamber pressure, is determined by the use of a digital timer-counter. The system has been used with fiber Fabry-Perot interferometer transducers for in-cylinder pressure measurement on a four-cylinder gasoline-powered engine.

  3. Probing the effect of intrinsic defects and dopants on the structural evolution and optical properties of ZnO nanocrystallites

    SciTech Connect

    Panda, N. R.; Sahu, D.; Acharya, B. S.; Nayak, P.

    2015-06-24

    Role of intrinsic defects and external impurities in modifying the structural and optical properties of ZnO nanostructures has been studied and discussed. ZnO nanocrystallites doped with B, N and S elements have been prepared by ultrasound assisted wet chemical method. Structural evolution of ZnO in presence of dopant ions has been studied by XRD and electron microscopic measurements. Elemental analysis like XPS has been carried out to ascertain the dopant configuration. A variation in crystallographic parameters and microstructure is found to be observed as impurity is incorporated into ZnO. This has been explained on the basis of the substitution of dopant at Zn{sup 2+} and O{sup −} sites rearranging the lattice. Optical absorption measurements and PL studies reflect a change in band gap of ZnO by impurity adsorption. Most of the cases, the band gap is found to be broadened which has been explained in the line of Moss-Burstein effect. The excitonic emission in ZnO is observed to blue shift supporting the above results and the defect emissions also get modified in terms of position and intensity. New PL bands observed have been assigned to the transitions related to the defect states present in the band gap of ZnO along with intrinsic defects.

  4. Interchangeability of Caenorhabditis elegans DSL proteins and intrinsic signalling activity of their extracellular domains in vivo.

    PubMed

    Fitzgerald, K; Greenwald, I

    1995-12-01

    Ligands of the Delta/Serrate/lag-2 (DSL) family and their receptors, members of the lin-12/Notch family, mediate cell-cell interactions that specify cell fate in invertebrates and vertebrates. In C. elegans, two DSL genes, lag-2 and apx-1, influence different cell fate decisions during development. Here we show that APX-1 can fully substitute for LAG-2 when expressed under the control of lag-2 regulatory sequences. In addition, we demonstrate that truncated forms lacking the transmembrane and intracellular domains of both LAG-2 and APX-1 can also substitute for endogenous lag-2 activity. Moreover, we provide evidence that these truncated forms are secreted and able to activate LIN-12 and GLP-1 ectopically. Finally, we show that expression of a secreted DSL domain alone may enhance endogenous LAG-2 signalling. Our data suggest ways that activated forms of DSL ligands in other systems may be created.

  5. Collective mesendoderm migration relies on an intrinsic directionality signal transmitted through cell contacts.

    PubMed

    Dumortier, Julien G; Martin, Sandro; Meyer, Dirk; Rosa, Frederic M; David, Nicolas B

    2012-10-16

    Collective cell migration is key to morphogenesis, wound healing, or cancer cell migration. However, its cellular bases are just starting to be unraveled. During vertebrate gastrulation, axial mesendoderm migrates in a group, the prechordal plate, from the embryonic organizer to the animal pole. How this collective migration is achieved remains unclear. Previous work has suggested that cells migrate as individuals, with collective movement resulting from the addition of similar individual cell behavior. Through extensive analyses of cell trajectories, morphologies, and polarization in zebrafish embryos, we reveal that all prechordal plate cells show the same behavior and rely on the same signaling pathway to migrate, as expected if they do so individually. However, by using cell transplants, we demonstrate that prechordal plate migration is a true collective process, as isolated cells do not migrate toward the animal pole. They are still polarized and motile but lose directionality. Directionality is restored upon contact with the endogenous prechordal plate. This contact dependent orientation relies on E-cadherin, Wnt-PCP signaling, and Rac1. Importantly, groups of cells also need contact with the endogenous plate to orient correctly, showing an instructive role of the plate in establishing directionality. Overall, our results lead to an original model of collective migration in which directional information is contained within the moving group rather than provided by extrinsic cues, and constantly maintained in cells by contacts with their neighbors. This self-organizing model could account for collective invasion of new territories, as observed in cancer strands, without requirement for any attractant in the colonized tissue.

  6. Free space optical communications through clouds: analysis of signal characteristics.

    PubMed

    Wu, Binbin; Hajjarian, Zeinab; Kavehrad, Mohsen

    2008-06-10

    Free space optical communications (FSOC) is a method by which one transmits a modulated beam of light through the atmosphere for broadband applications. Fundamental limitations of FSOC arise from the environment through which light propagates. This work addresses transmitted light beam dispersion (spatial, angular, and temporal dispersion) in FSOC operating as a ground-to-air link when clouds exist along the communications channel. Light signals (photons) transmitted through clouds will interact with the cloud particles. Photon-particle interaction causes dispersion of light signals, which has significant effects on signal attenuation and pulse spread. The correlation between spatial and angular dispersion is investigated as well, which plays an important role on the receiver design. Moreover, the paper indicates that temporal dispersion (pulse spread) and energy loss strongly depend on the aperture size of the receiver, the field-of-view (FOV), and the position of the receiver relative to the optical axis of the transmitter.

  7. Bacteriorhodopsin films for optical signal processing and data storage

    NASA Technical Reports Server (NTRS)

    Walkup, John F. (Principal Investigator); Mehrl, David J. (Principal Investigator)

    1996-01-01

    This report summarizes the research results obtained on NASA Ames Grant NAG 2-878 entitled 'Investigations of Bacteriorhodopsin Films for Optical Signal Processing and Data Storage.' Specifically we performed research, at Texas Tech University, on applications of Bacteriorhodopisin film to both (1) dynamic spatial filtering and (2) holographic data storage. In addition, measurements of the noise properties of an acousto-optical matrix-vestor multiplier built for NASA Ames by Photonic Systems Inc. were performed at NASA Ames' Photonics Laboratory. This research resulted in two papers presented at major optical data processing conferences and a journal paper which is to appear in APPLIED OPTICS. A new proposal for additional BR research has recently been submitted to NASA Ames Research Center.

  8. Intrinsic low pass filtering improves signal-to-noise ratio in critical-point flexure biosensors

    SciTech Connect

    Jain, Ankit; Alam, Muhammad Ashraful

    2014-08-25

    A flexure biosensor consists of a suspended beam and a fixed bottom electrode. The adsorption of the target biomolecules on the beam changes its stiffness and results in change of beam's deflection. It is now well established that the sensitivity of sensor is maximized close to the pull-in instability point, where effective stiffness of the beam vanishes. The question: “Do the signal-to-noise ratio (SNR) and the limit-of-detection (LOD) also improve close to the instability point?”, however remains unanswered. In this article, we systematically analyze the noise response to evaluate SNR and establish LOD of critical-point flexure sensors. We find that a flexure sensor acts like an effective low pass filter close to the instability point due to its relatively small resonance frequency, and rejects high frequency noise, leading to improved SNR and LOD. We believe that our conclusions should establish the uniqueness and the technological relevance of critical-point biosensors.

  9. Multiple-channel optical signal processing with wavelength-waveform conversions, pulsewidth tunability, and signal regeneration.

    PubMed

    Nguyen Tan, Hung; Matsuura, Motoharu; Katafuchi, Tomoya; Kishi, Naoto

    2009-12-01

    A multiple-channel multiple-function optical signal processor (MCMF-OSP) including wavelength-waveform conversions, pulsewidth tunability, and signal regeneration is realized through AND logic gate based on optical parametric processing with a pulsewidth-tunable RZ clock pump. The proposed scheme simultaneously offers four signal processing functions which are useful in wavelength-division multiplexing (WDM) transmission systems, and at network nodes with the necessity for multiple-channel data processing. After the discussions on the concept of MCMF-OSP, a proof-of concept experiment is demonstrated on four 10 Gb/s nonreturn-to-zero (NRZ) data format channels using nonlinearities in semiconductor optical amplifier (SOA) and highly nonlinear fiber (HNLF). A wavelength and waveform conversions to return-to-zero (RZ) modulation format are obtained together with pulsewidth-tunable range from 20% to 80% duty cycles for all input signals. The converted signals inherit the timing and waveform of the RZ clock pump, thus resulting in a time regeneration and large tolerance to narrow-band optical filtering (NAOF) and fiber accumulated chromatic dispersion (CD). PMID:20052222

  10. Cinobufagin inhibits tumor growth by inducing intrinsic apoptosis through AKT signaling pathway in human nonsmall cell lung cancer cells

    PubMed Central

    Zhang, Guangxin; Wang, Chao; Sun, Mei; Li, Jindong; Wang, Bin; Jin, Chengyan; Hua, Peiyan; Song, Ge; Zhang, Yifan; Nguyen, Lisa L.H.; Cui, Ranji; Liu, Runhua; Wang, Lizhong; Zhang, Xingyi

    2016-01-01

    The cinobufagin (CB) has a broad spectrum of cytotoxicity to inhibit cell proliferation of various human cancer cell lines, but the molecular mechanisms still remain elusive. Here we observed that CB inhibited the cell proliferation and tumor growth, but induced cell cycle arrest and apoptosis in a dose-dependent manner in non-small cell lung cancer (NSCLC) cells. Treatment with CB significantly increased the reactive oxygen species but decreased the mitochondrial membrane potential in NSCLC cells. These effects were markedly blocked when the cells were pretreated with N-acetylcysteine, a specific reactive oxygen species inhibitor. Furthermore, treatment with CB induced the expression of BAX but reduced that of BCL-2, BCL-XL and MCL-1, leading to an activation of caspase-3, chromatin condensation and DNA degradation in order to induce programmed cell death in NSCLC cells. In addition, treatment with CB reduced the expressions of p-AKTT308 and p-AKTS473 and inhibited the AKT/mTOR signaling pathway in NSCLC cells in a time-dependent manner. Our results suggest that CB inhibits tumor growth by inducing intrinsic apoptosis through the AKT signaling pathway in NSCLC cells. PMID:26959116

  11. Cordycepin induces apoptosis in human liver cancer HepG2 cells through extrinsic and intrinsic signaling pathways

    PubMed Central

    Shao, Le-Wen; Huang, Li-Hua; Yan, Sheng; Jin, Jian-Di; Ren, Shao-Yan

    2016-01-01

    Cordycepin, also termed 3′-deoxyadenosine, is a nucleoside analogue from Cordyceps sinensis and has been reported to demonstrate numerous biological and pharmacological properties. Our previous study illustrated that the anti-tumor effect of cordycepin may be associated with apoptosis. In the present study, the apoptotic effect of cordycepin on HepG2 cells was investigated using 4′,6-diamidino-2-phenylindole, tetraethylbenzimidazolylcarbocyanine iodide and propidium iodide staining analysis and flow cytometry. The results showed that cordycepin exhibited the ability to inhibit HepG2 cells in a time- and dose-dependent manner when cells produced typical apoptotic morphological changes, including chromatin condensation, the accumulation of sub-G1 cells and change mitochondrial permeability. A potential mechanism for cordycepin-induced apoptosis of human liver cancer HepG2 cells may occur through the extrinsic signaling pathway mediated by the transmembrane Fas-associated with death domain protein. Apoptosis was also associated with Bcl-2 family protein regulation, leading to altered mitochondrial membrane permeability and resulting in the release of cytochrome c into the cytosol. The activation of the caspase cascade is responsible for the execution of apoptosis. In conclusion, cordycepin-induced apoptosis in HepG2 cells involved the extrinsic and intrinsic signaling pathway and was primarily regulated by the Bcl-2 family proteins. PMID:27446383

  12. SERODS optical data storage with parallel signal transfer

    DOEpatents

    Vo-Dinh, Tuan

    2003-06-24

    Surface-enhanced Raman optical data storage (SERODS) systems having increased reading and writing speeds, that is, increased data transfer rates, are disclosed. In the various SERODS read and write systems, the surface-enhanced Raman scattering (SERS) data is written and read using a two-dimensional process called parallel signal transfer (PST). The various embodiments utilize laser light beam excitation of the SERODS medium, optical filtering, beam imaging, and two-dimensional light detection. Two- and three-dimensional SERODS media are utilized. The SERODS write systems employ either a different laser or a different level of laser power.

  13. SERODS optical data storage with parallel signal transfer

    DOEpatents

    Vo-Dinh, Tuan

    2003-09-02

    Surface-enhanced Raman optical data storage (SERODS) systems having increased reading and writing speeds, that is, increased data transfer rates, are disclosed. In the various SERODS read and write systems, the surface-enhanced Raman scattering (SERS) data is written and read using a two-dimensional process called parallel signal transfer (PST). The various embodiments utilize laser light beam excitation of the SERODS medium, optical filtering, beam imaging, and two-dimensional light detection. Two- and three-dimensional SERODS media are utilized. The SERODS write systems employ either a different laser or a different level of laser power.

  14. Cell-intrinsic gp130 Signaling on CD4+ T cells Shapes Long-lasting Antiviral Immunity

    PubMed Central

    Harker, James A.; Wong, Kurt A.; Dolgoter, Aleksandr; Zuniga, Elina I.

    2015-01-01

    The interleukin-6 (IL-6) cytokine family utilizes the common signal transduction molecule gp130, which can mediate a diverse range of outcomes. To clarify the role of gp130 signaling in vivo during acute viral infection we infected Cd4-cre Il6stfl/fl mice, in which gp130 is conditionally ablated in T cells, with acute lymphocytic choriomeningitis virus (LCMV). We found that by day 12, but not at day 8, post infection the number of virus specific CD4+ T cells was reduced in the absence of gp130, and this was sustained for up to 2 months post infection. Additionally gp130 deficient TFH had lower expression of Maf, IL-21 and ICOS and this was accompanied by a reduction in the proportion of germinal center B cells and plasmablasts. Remarkably, two months post-infection the proportion of IgG2a/c+ memory B cells and the systemic levels of LCMV-specific IgG2 Abs were dramatically decreased, while there was a corresponding increase in IgG1+ memory B cells and virus-specific IgG1 Abs. In the same animals Gp130 deficient virus specific CD8+ T cells showed a reduced proportion of memory cells, which expressed lower levels of Tcf7, and displayed diminished recall responses on secondary infection. Mixed bone marrow chimeras revealed that the aforementioned gp130 effects on CD4+ T cells were cell-intrinsic. Overall our data show that gp130 signaling in T cells influences the quantity and quality of long lasting CD4+ T cell responses as well as CD8+ T cell and antibody mediated immunity after acute viral infection. PMID:26085685

  15. How Intrinsic Molecular Dynamics Control Intramolecular Communication in Signal Transducers and Activators of Transcription Factor STAT5

    PubMed Central

    Langenfeld, Florent; Guarracino, Yann; Arock, Michel; Trouvé, Alain; Tchertanov, Luba

    2015-01-01

    Signal Transducer and Activator of Transcription STAT5 is a key mediator of cell proliferation, differentiation and survival. While STAT5 activity is tightly regulated in normal cells, its constitutive activation directly contributes to oncogenesis and is associated with a broad range of hematological and solid tumor cancers. Therefore the development of compounds able to modulate pathogenic activation of this protein is a very challenging endeavor. A crucial step of drug design is the understanding of the protein conformational features and the definition of putative binding site(s) for such modulators. Currently, there is no structural data available for human STAT5 and our study is the first footprint towards the description of structure and dynamics of this protein. We investigated structural and dynamical features of the two STAT5 isoforms, STAT5a and STAT5b, taken into account their phosphorylation status. The study was based on the exploration of molecular dynamics simulations by different analytical methods. Despite the overall folding similarity of STAT5 proteins, the MD conformations display specific structural and dynamical features for each protein, indicating first, sequence-encoded structural properties and second, phosphorylation-induced effects which contribute to local and long-distance structural rearrangements interpreted as allosteric event. Further examination of the dynamical coupling between distant sites provides evidence for alternative profiles of the communication pathways inside and between the STAT5 domains. These results add a new insight to the understanding of the crucial role of intrinsic molecular dynamics in mediating intramolecular signaling in STAT5. Two pockets, localized in close proximity to the phosphotyrosine-binding site and adjacent to the channel for communication pathways across STAT5, may constitute valid targets to develop inhibitors able to modulate the function-related communication properties of this signaling

  16. Enhancement of Second-Order Nonlinear-Optical Signals by Optical Stimulation

    NASA Astrophysics Data System (ADS)

    Goodman, A. J.; Tisdale, W. A.

    2015-05-01

    Second-order nonlinear optical interactions such as sum- and difference-frequency generation are widely used for bioimaging and as selective probes of interfacial environments. However, inefficient nonlinear optical conversion often leads to poor signal-to-noise ratio and long signal acquisition times. Here, we demonstrate the dramatic enhancement of weak second-order nonlinear optical signals via stimulated sum- and difference-frequency generation. We present a conceptual framework to quantitatively describe the interaction and show that the process is highly sensitive to the relative optical phase of the stimulating field. To emphasize the utility of the technique, we demonstrate stimulated enhancement of second harmonic generation (SHG) from bovine collagen-I fibrils. Using a stimulating pulse fluence of only 3 nJ /cm2 , we obtain an SHG enhancement >104 relative to the spontaneous signal. The stimulation enhancement is greatest in situations where spontaneous signals are the weakest—such as low laser power, small sample volume, and weak nonlinear susceptibility—emphasizing the potential for this technique to improve signal-to-noise ratios in biological imaging and interfacial spectroscopy.

  17. Ultrafast optical signal processing on silicon-based platforms

    NASA Astrophysics Data System (ADS)

    Tan, Dawn T. H.

    2016-03-01

    The development of silicon - based photonic components and systems has advanced tremendously over the last decade, largely for applications in optical interconnects. The role of silicon - based platforms for both linear and nonlinear optics remains highly pertinent because of their ability to be integrated with CMOS - based electronics. In this paper, we present recent research progress pertaining to ultrafast optical signal processing on silicon - based platforms. Advances in on - chip multiplexing strategies with the potential for meeting 200GHz dense wavelength division multiplexing standards across the C - and L - bands will be discussed. In addition, the development of a silicon - based nonlinear optics platform with high nonlinear figures of merit will be presented. Nonlinear optical devices fabricated from the developed platform possess nonlinear parameters 500 times larger than that in silicon nitride waveguides, while possessing negligible nonlinear losses at 1.55μm. Ultra - broadband, low power nonlinear wavelength generation using these devices, as well as their potential for realizing advanced light sources for optical interconnect - based applications will be presented.

  18. Intrinsic Differences in the Inner Jets of High and Low Optically Polarized Radio Quasars

    NASA Technical Reports Server (NTRS)

    Lister, M.; Smith, P.

    2000-01-01

    We have conducted a high-resolution polarization study with the VLBA at 22 and 43 GHz to look for differences in the parsec-scale magnetic field structures of 18 high- and low-optically polarized, compact radio-loud quasars (HPQs and LPRQs, respectively).

  19. Intrinsic artefacts in optical oxygen sensors--how reliable are our measurements?

    PubMed

    Lehner, Philipp; Staudinger, Christoph; Borisov, Sergey M; Regensburger, Johannes; Klimant, Ingo

    2015-03-01

    Optical oxygen sensing is of broad interest in many areas of research, such as medicine, food processing, and micro- and marine biology. The operation principle of optical oxygen sensors is well established and these sensors are routinely employed in lab and field experiments. Ultratrace oxygen sensors, which enable measurements in the sub-nanomolar region (dissolved oxygen), are becoming increasingly important. Such sensors prominently exhibit phenomena that complicate calibration and measurements. However, these phenomena are not constrained to ultratrace sensors; rather, these effects are inherent to the way optical oxygen sensors work and may influence any optical oxygen measurement when certain conditions are met. This scenario is especially true for applications that deal with high-excitation light intensities, such as microscopy and microfluidic applications. Herein, we present various effects that we could observe in our studies with ultratrace oxygen sensors and discuss the reasons for their appearance, the mechanism by which they influence measurements, and how to best reduce their impact. The phenomena discussed are oxygen photoconsumption in the sensor material; depletion of the dye ground state by high-excitation photon-flux values, which can compromise both intensity and ratiometric-based measurements; triplet-triplet annihilation; and singlet-oxygen accumulation, which affects measurements at very low oxygen concentrations.

  20. Toward optical signal processing using photonic reservoir computing.

    PubMed

    Vandoorne, Kristof; Dierckx, Wouter; Schrauwen, Benjamin; Verstraeten, David; Baets, Roel; Bienstman, Peter; Van Campenhout, Jan

    2008-07-21

    We propose photonic reservoir computing as a new approach to optical signal processing in the context of large scale pattern recognition problems. Photonic reservoir computing is a photonic implementation of the recently proposed reservoir computing concept, where the dynamics of a network of nonlinear elements are exploited to perform general signal processing tasks. In our proposed photonic implementation, we employ a network of coupled Semiconductor Optical Amplifiers (SOA) as the basic building blocks for the reservoir. Although they differ in many key respects from traditional software-based hyperbolic tangent reservoirs, we show using simulations that such a photonic reservoir can outperform traditional reservoirs on a benchmark classification task. Moreover, a photonic implementation offers the promise of massively parallel information processing with low power and high speed.

  1. Storage and switching of multiple optical signals among three channels

    SciTech Connect

    Song Xiaoli; Li Aijun; Wang Lei; Kang Zhihui; Kou Jun; Wang Chunliang; Jiang Yun; Gao Jinyue; Zhang Bing

    2009-05-15

    We experimentally and theoretically demonstrate that multioptical signals can be effectively stored and retrieved by fractional stimulated Raman adiabatic passage technique in a tripod-type four-level {sup 87}Rb atomic system. The optical pulses stored can be controllably released into two of the three different channels. The restored pulses have the same frequency, polarization, and propagation direction as the writing pulses. The experimental results fit very well with the numerical simulations.

  2. Tomography of the near-field optical signal.

    PubMed

    Grosges, Thomas; Barchiesi, Dominique

    2006-12-01

    In near-field optics, measurement of vertical variations of the near field is of great interest for characterizing the efficiency of resonances such as surface plasmon polaritons. The use of the signal obtained through the lock-in amplifier using a feedback on the vertical vibration of the probe is shown to enable the reconstruction of the near field without the use of the slower technique of approach curves. Therefore, a tomography of the near field is directly available.

  3. Optical communication system performance with tracking error induced signal fading.

    NASA Technical Reports Server (NTRS)

    Tycz, M.; Fitzmaurice, M. W.; Premo, D. A.

    1973-01-01

    System performance is determined for an optical communication system using noncoherent detection in the presence of tracking error induced signal fading assuming (1) binary on-off modulation (OOK) with both fixed and adaptive threshold receivers, and (2) binary polarization modulation (BPM). BPM is shown to maintain its inherent 2- to 3-dB advantage over OOK when adaptive thresholding is used, and to have a substantially greater advantage when the OOK system is restricted to a fixed decision threshold.

  4. Causal evidence that intrinsic beta-frequency is relevant for enhanced signal propagation in the motor system as shown through rhythmic TMS.

    PubMed

    Romei, Vincenzo; Bauer, Markus; Brooks, Joseph L; Economides, Marcos; Penny, Will; Thut, Gregor; Driver, Jon; Bestmann, Sven

    2016-02-01

    Correlative evidence provides support for the idea that brain oscillations underpin neural computations. Recent work using rhythmic stimulation techniques in humans provide causal evidence but the interactions of these external signals with intrinsic rhythmicity remain unclear. Here, we show that sensorimotor cortex follows externally applied rhythmic TMS (rTMS) stimulation in the beta-band but that the elicited responses are strongest at the intrinsic individual beta peak frequency. While these entrainment effects are of short duration, even subthreshold rTMS pulses propagate through the network and elicit significant cortico-spinal coupling, particularly when stimulated at the individual beta-frequency. Our results show that externally enforced rhythmicity interacts with intrinsic brain rhythms such that the individual peak frequency determines the effect of rTMS. The observed downstream spinal effect at the resonance frequency provides evidence for the causal role of brain rhythms for signal propagation. PMID:26584867

  5. Causal evidence that intrinsic beta-frequency is relevant for enhanced signal propagation in the motor system as shown through rhythmic TMS

    PubMed Central

    Romei, Vincenzo; Bauer, Markus; Brooks, Joseph L.; Economides, Marcos; Penny, Will; Thut, Gregor; Driver, Jon; Bestmann, Sven

    2016-01-01

    Correlative evidence provides support for the idea that brain oscillations underpin neural computations. Recent work using rhythmic stimulation techniques in humans provide causal evidence but the interactions of these external signals with intrinsic rhythmicity remain unclear. Here, we show that sensorimotor cortex follows externally applied rhythmic TMS (rTMS) stimulation in the beta-band but that the elicited responses are strongest at the intrinsic individual beta peak frequency. While these entrainment effects are of short duration, even subthreshold rTMS pulses propagate through the network and elicit significant cortico-spinal coupling, particularly when stimulated at the individual beta-frequency. Our results show that externally enforced rhythmicity interacts with intrinsic brain rhythms such that the individual peak frequency determines the effect of rTMS. The observed downstream spinal effect at the resonance frequency provides evidence for the causal role of brain rhythms for signal propagation. PMID:26584867

  6. Multi-functional optical signal processing using optical spectrum control circuit

    NASA Astrophysics Data System (ADS)

    Hayashi, Shuhei; Ikeda, Tatsuhiko; Mizuno, Takayuki; Takahashi, Hiroshi; Tsuda, Hiroyuki

    2015-02-01

    Processing ultra-fast optical signals without optical/electronic conversion is in demand and time-to-space conversion has been proposed as an effective solution. We have designed and fabricated an arrayed-waveguide grating (AWG) based optical spectrum control circuit (OSCC) using silica planar lightwave circuit (PLC) technology. This device is composed of an AWG, tunable phase shifters and a mirror. The principle of signal processing is to spatially decompose the signal's frequency components by using the AWG. Then, the phase of each frequency component is controlled by the tunable phase shifters. Finally, the light is reflected back to the AWG by the mirror and synthesized. Amplitude of each frequency component can be controlled by distributing the power to high diffraction order light. The spectral controlling range of the OSCC is 100 GHz and its resolution is 1.67 GHz. This paper describes equipping the OSCC with optical coded division multiplex (OCDM) encoder/decoder functionality. The encoding principle is to apply certain phase patterns to the signal's frequency components and intentionally disperse the signal. The decoding principle is also to apply certain phase patterns to the frequency components at the receiving side. If the applied phase pattern compensates the intentional dispersion, the waveform is regenerated, but if the pattern is not appropriate, the waveform remains dispersed. We also propose an arbitrary filter function by exploiting the OSCC's amplitude and phase control attributes. For example, a filtered optical signal transmitted through multiple optical nodes that use the wavelength multiplexer/demultiplexer can be equalized.

  7. Embodiment of Learning in Electro-Optical Signal Processors

    NASA Astrophysics Data System (ADS)

    Hermans, Michiel; Antonik, Piotr; Haelterman, Marc; Massar, Serge

    2016-09-01

    Delay-coupled electro-optical systems have received much attention for their dynamical properties and their potential use in signal processing. In particular it has recently been demonstrated, using the artificial intelligence algorithm known as reservoir computing, that photonic implementations of such systems solve complex tasks such as speech recognition. Here we show how the backpropagation algorithm can be physically implemented on the same electro-optical delay-coupled architecture used for computation with only minor changes to the original design. We find that, compared when the backpropagation algorithm is not used, the error rate of the resulting computing device, evaluated on three benchmark tasks, decreases considerably. This demonstrates that electro-optical analog computers can embody a large part of their own training process, allowing them to be applied to new, more difficult tasks.

  8. Method and means for detecting optically transmitted signals and establishing optical interference pattern between electrodes

    DOEpatents

    Kostenbauder, Adnah G.

    1988-01-01

    A photodetector for detecting signal pulses transmitted in an optical carrier signal relies on the generation of electron-hole pairs and the diffusion of the generated electrons and holes to the electrodes on the surface of the semiconductor detector body for generating photovoltaic pulses. The detector utilizes the interference of optical waves for generating an electron-hole grating within the semiconductor body, and, by establishing an electron-hole pair maximum at one electrode and a minimum at the other electrode, a detectable voltaic pulse is generated across the electrode.

  9. Method and means for detecting optically transmitted signals and establishing optical interference pattern between electrodes

    DOEpatents

    Kostenbauder, A.G.

    1988-06-28

    A photodetector for detecting signal pulses transmitted in an optical carrier signal relies on the generation of electron-hole pairs and the diffusion of the generated electrons and holes to the electrodes on the surface of the semiconductor detector body for generating photovoltaic pulses. The detector utilizes the interference of optical waves for generating an electron-hole grating within the semiconductor body, and, by establishing an electron-hole pair maximum at one electrode and a minimum at the other electrode, a detectable voltaic pulse is generated across the electrode. 4 figs.

  10. Intrinsic carrier multiplication efficiency in bulk Si crystals evaluated by optical-pump/terahertz-probe spectroscopy

    SciTech Connect

    Yamashita, G.; Nagai, M. E-mail: ashida@mp.es.osaka-u.ac.jp; Ashida, M. E-mail: ashida@mp.es.osaka-u.ac.jp; Matsubara, E.; Kanemitsu, Y.

    2014-12-08

    We estimated the carrier multiplication efficiency in the most common solar-cell material, Si, by using optical-pump/terahertz-probe spectroscopy. Through close analysis of time-resolved data, we extracted the exact number of photoexcited carriers from the sheet carrier density 10 ps after photoexcitation, excluding the influences of spatial diffusion and surface recombination in the time domain. For incident photon energies greater than 4.0 eV, we observed enhanced internal quantum efficiency due to carrier multiplication. The evaluated value of internal quantum efficiency agrees well with the results of photocurrent measurements. This optical method allows us to estimate the carrier multiplication and surface recombination of carriers quantitatively, which are crucial for the design of the solar cells.

  11. Optical two-tone signal generation without use of optical filter for photonics-assisted radio-frequency quadrupling.

    PubMed

    Chiba, Akito; Akamatsu, Yosuke; Takada, Kazumasa

    2015-08-01

    Optical two-tone (OTT) signal generation is demonstrated without optical wavelength filtering for wavelength-free operation in radio-frequency (RF) upconversion assisted by photonics. This principle is based on selective polarization manipulation for the optical carrier; the optical carrier's polarization is first tilted, and the carrier is then suppressed using a polarizer. Owing to optimized conditions obtained from theoretical calculation and the high polarization extinction ratio achieving a 25.7-dB carrier suppression, a 40-GHz separated OTT signal is successfully generated by an optical intensity modulator driven by a 10-GHz sinusoidal RF signal. Conversion into a frequency-quadrupled RF signal is also demonstrated experimentally.

  12. Probing the intrinsic optical Bloch-mode emission from a 3D photonic crystal

    NASA Astrophysics Data System (ADS)

    Hsieh, Mei-Li; Bur, James A.; Du, Qingguo; John, Sajeev; Lin, Shawn-Yu

    2016-10-01

    We report experimental observation of intrinsic Bloch-mode emission from a 3D tungsten photonic crystal at low thermal excitation. After the successful removal of conventional metallic emission (normal emission), it is possible to make an accurate comparison of the Bloch-mode and the normal emission. For all biases, we found that the emission intensity of the Bloch-mode is higher than that of the normal emission. The Bloch-mode emission also exhibits a slower dependence on (\\hslash ω /{k}bT) than that of the normal emission. The observed higher emission intensity and a different T-dependence is attributed to Bloch-mode assisted emission where emitters have been located into a medium having local density of states different than the isotropic case. Furthermore, our finite-difference time-domain (FDTD) simulation shows the presence of localized spots at metal-air boundaries and corners, having intense electric field. The enhanced plasmonic field and local non-equilibrium could induce a strong thermally stimulated emission and may be the cause of our unusual observation.

  13. Following intracellular cholesterol transport by linear and non-linear optical microscopy of intrinsically fluorescent sterols.

    PubMed

    Wüstner, Daniel

    2012-02-01

    Elucidation of intracellular cholesterol transport is important for understanding the molecular basis of several metabolic and neuronal diseases, like atheroclerosis or lysosomal storage disorders. Progress in this field depends crucially on the development of new technical approaches to follow the cellular movement of this essential lipid molecule. In this article, a survey of the various methods being used for analysis of sterol trafficking is given. Various classical biochemical methods are presented and their suitability for analysis of sterol trafficking is assessed. Special emphasis is on recent developments in imaging technology to follow the intracellular fate of intrinsically fluorescent sterols as faithful cholesterol markers. In particular, UV-sensitive wide field and multiphoton microscopy of the sterol dehydroergosterol, DHE, is explained and new methods of quantitative image analysis like pixel-wise bleach rate fitting and multiphoton image correlation spectroscopy are introduced. Several applications of the new technology including observation of vectorial sterol trafficking in polarized human hepatoma cells for investigation of reverse cholesterol transport are presented. PMID:21470123

  14. Probing the intrinsic optical Bloch-mode emission from a 3D photonic crystal.

    PubMed

    Hsieh, Mei-Li; Bur, James A; Du, Qingguo; John, Sajeev; Lin, Shawn-Yu

    2016-10-14

    We report experimental observation of intrinsic Bloch-mode emission from a 3D tungsten photonic crystal at low thermal excitation. After the successful removal of conventional metallic emission (normal emission), it is possible to make an accurate comparison of the Bloch-mode and the normal emission. For all biases, we found that the emission intensity of the Bloch-mode is higher than that of the normal emission. The Bloch-mode emission also exhibits a slower dependence on [Formula: see text] than that of the normal emission. The observed higher emission intensity and a different T-dependence is attributed to Bloch-mode assisted emission where emitters have been located into a medium having local density of states different than the isotropic case. Furthermore, our finite-difference time-domain (FDTD) simulation shows the presence of localized spots at metal-air boundaries and corners, having intense electric field. The enhanced plasmonic field and local non-equilibrium could induce a strong thermally stimulated emission and may be the cause of our unusual observation. PMID:27606574

  15. Probing the intrinsic optical Bloch-mode emission from a 3D photonic crystal.

    PubMed

    Hsieh, Mei-Li; Bur, James A; Du, Qingguo; John, Sajeev; Lin, Shawn-Yu

    2016-10-14

    We report experimental observation of intrinsic Bloch-mode emission from a 3D tungsten photonic crystal at low thermal excitation. After the successful removal of conventional metallic emission (normal emission), it is possible to make an accurate comparison of the Bloch-mode and the normal emission. For all biases, we found that the emission intensity of the Bloch-mode is higher than that of the normal emission. The Bloch-mode emission also exhibits a slower dependence on [Formula: see text] than that of the normal emission. The observed higher emission intensity and a different T-dependence is attributed to Bloch-mode assisted emission where emitters have been located into a medium having local density of states different than the isotropic case. Furthermore, our finite-difference time-domain (FDTD) simulation shows the presence of localized spots at metal-air boundaries and corners, having intense electric field. The enhanced plasmonic field and local non-equilibrium could induce a strong thermally stimulated emission and may be the cause of our unusual observation.

  16. Oxaliplatin-chitosan nanoparticles induced intrinsic apoptotic signaling pathway: a "smart" drug delivery system to breast cancer cell therapy.

    PubMed

    Vivek, Raju; Thangam, Ramar; Nipunbabu, Varukattu; Ponraj, Thondhi; Kannan, Soundarapandian

    2014-04-01

    This study was to investigate "smart" pH-responsive drug delivery system (DDS) based on chitosan nano-carrier for its potential intelligent controlled release and enhancing chemotherapeutic efficiency of Oxalipaltin. Oxaliplatin was loaded onto chitosan by forming complexes with degradable to construct nano-carrier as a DDS. Oxaliplatin was released from the DDS much more rapidly at pH 4.5 than at pH 7.4, which is a desirable characteristic for tumor-targeted drug delivery. Furthermore, the possible intrinsic apoptotic signaling pathway was explored by Western blot. It was found that expression of Bax, Bik, cytochrome C, caspase-9 and -3 was significantly up-regulated while the Bcl-2 and Survivin were inhibited in breast cancer MCF-7 cells. For instance, nanoparticles inducing apoptosis in caspase-dependent manner indicate that chitosan nanoparticles could act as an efficient DDS importing Oxalipaltin to target cancer cells. These approaches suggest that "smart" Oxaliplatin delivery strategy is a promising approach to cancer therapy.

  17. Bufalin reverses intrinsic and acquired drug resistance to cisplatin through the AKT signaling pathway in gastric cancer cells.

    PubMed

    Zhao, Hongyan; Zhao, Dali; Jin, Huilin; Li, Hongwei; Yang, Xiaoying; Zhuang, Liwei; Liu, Tiefu

    2016-08-01

    Cisplatin is the most common chemotherapeutic agent for gastric cancer (GC), however it activates AKT, which contributes to intrinsic and acquired resistance. Bufalin, a traditional Chinese medicine, shows significant anticancer activity by inhibiting the AKT pathway. It was therefore hypothesized that bufalin could counteract cisplatin resistance in GC cells. SGC7901, MKN‑45 and BGC823 human GC cells were cultured under normoxic and hypoxic conditions. Effects of cisplatin and bufalin on GC cells were measured by a cell counting kit, apoptosis was analyzed by flow cytometry, and immunoblotting was used to detect proteins associated with the AKT signaling pathway. It was demonstrated that bufalin synergized with cisplatin to inhibit proliferation and promote apoptosis of GC cells by diminishing the activation of cisplatin-induced AKT under normoxic and hypoxic conditions. Bufalin also inhibits cisplatin-activated molecules downstream of AKT that affect proliferation and apoptosis, including glycogen synthase kinase, mammalian target of rapamycin, ribosomal protein S6 Kinase and eukaryotic translation initiation factor-4E-binding protein-1. To investigate acquired cisplatin resistance, a cisplatin‑resistant cell line SGC7901‑CR was used. It was demonstrated that bufalin reversed acquired cisplatin resistance and significantly induced apoptosis through the AKT pathway. These results imply that bufalin could extend the therapeutic effect of cisplatin on GC cells when administered in combination. PMID:27357249

  18. Effects of Linker Length and Transient Secondary Structure Elements in the Intrinsically Disordered Notch RAM Region on Notch Signaling.

    PubMed

    Sherry, Kathryn P; Johnson, Scott E; Hatem, Christine L; Majumdar, Ananya; Barrick, Doug

    2015-11-01

    Formation of the bivalent interaction between the Notch intracellular domain (NICD) and the transcription factor CBF-1/RBP-j, Su(H), Lag-1 (CSL) is a key event in Notch signaling because it switches Notch-responsive genes from a repressed state to an activated state. Interaction of the intrinsically disordered RBP-j-associated molecule (RAM) region of NICD with CSL is thought to both disrupt binding of corepressor proteins to CSL and anchor NICD to CSL, promoting interaction of the ankyrin domain of NICD with CSL through an effective concentration mechanism. To quantify the role of disorder in the RAM linker region on the effective concentration enhancement of Notch transcriptional activation, we measured the effects of linker length variation on activation. The resulting activation profile has general features of a worm-like chain model for effective concentration. However, deviations from the model for short sequence deletions suggest that RAM contains sequence-specific structural elements that may be important for activation. Structural characterization of the RAM linker with sedimentation velocity analytical ultracentrifugation and NMR spectroscopy reveals that the linker is compact and contains three transient helices and two extended and dynamic regions. To test if these secondary structure elements are important for activation, we made sequence substitutions to change the secondary structure propensities of these elements and measured transcriptional activation of the resulting variants. Substitutions to two of these nonrandom elements (helix 2, extended region 1) have effects on activation, but these effects do not depend on the nature of the substituting residues. Thus, the primary sequences of these elements, but not their secondary structures, are influencing signaling.

  19. Digital processing of RF signals from optical frequency combs

    NASA Astrophysics Data System (ADS)

    Cizek, Martin; Smid, Radek; Buchta, Zdeněk.; Mikel, Břetislav; Lazar, Josef; Cip, Ondřej

    2013-01-01

    The presented work is focused on digital processing of beat note signals from a femtosecond optical frequency comb. The levels of mixing products of single spectral components of the comb with CW laser sources are usually very low compared to products of mixing all the comb components together. RF counters are more likely to measure the frequency of the strongest spectral component rather than a weak beat note. Proposed experimental digital signal processing system solves this problem by analyzing the whole spectrum of the output RF signal and using software defined radio (SDR) algorithms. Our efforts concentrate in two main areas: Firstly, using digital servo-loop techniques for locking free running continuous laser sources on single components of the fs comb spectrum. Secondly, we are experimenting with digital signal processing of the RF beat note spectrum produced by f-2f 1 technique used for assessing the offset and repetition frequencies of the comb, resulting in digital servo-loop stabilization of the fs comb. Software capable of computing and analyzing the beat-note RF spectrums using FFT and peak detection was developed. A SDR algorithm performing phase demodulation on the f- 2f signal is used as a regulation error signal source for a digital phase-locked loop stabilizing the offset frequency of the fs comb.

  20. Applications of all optical signal processing for advanced optical modulation formats

    NASA Astrophysics Data System (ADS)

    Nuccio, Scott R.

    Increased data traffic demands, along with a continual push to minimize cost per bit, have recently motivated a paradigm shift away from traditional on-off keying (OOK) fiber transmission links towards systems utilizing more advanced modulation formats. In particular, modulation formats that utilize the phase of the optical signal, including differential phase shift keying (DPSK) and differential quadrature phase shift keying (DQPSK) along with polarization multiplexing (Pol-MUX), have recently emerged as the most popular means for transmitting information over long-haul and ultra-long haul fiber transmission systems. DPSK is motivated by an increase in receiver sensitivity compared to traditional OOK. DQPSK is motivated by a doubling of the spectral efficiency, along with increased tolerance to dispersion and nonlinear distortions. Coherent communications has also emerged as a primary means of transmitting and receiving optical data due to its support of formats that utilize both phase and amplitude to further increase the spectral efficiency (bits/sec/Hz) of the optical channel, including quadrature amplitude modulation (QAM). Polarization multiplexing of channels is a straight forward method to allow two channels to share the same wavelength by propagating on orthogonal polarization axis and is easily supported in coherent systems where the polarization tracking can be performed in the digital domain. Furthermore, the forthcoming IEEE 100 Gbit/s Ethernet Standard, 802.3ba, provides greater bandwidth, higher data rates, and supports a mixture of modulation formats. In particular, Pol-MUX (D)QPSK has grown in interest as the high spectral efficiency allows for 100 Gbit/s transmission while still occupying the current 50 GHz/channel allocation of current 10 Gbit/s OOK fiber systems. In this manner, 100 Gbit/s transfer speeds using current fiber links, amplifiers, and filters may be possible. In addition to advanced modulation formats, it is expected that optical

  1. All-optical signal processing at 10 GHz using a photonic crystal molecule

    SciTech Connect

    Combrié, Sylvain; Lehoucq, Gaëlle; Junay, Alexandra; De Rossi, Alfredo; Malaguti, Stefania; Bellanca, Gaetano; Trillo, Stefano; Ménager, Loic; Peter Reithmaier, Johann

    2013-11-04

    We report on 10 GHz operation of an all-optical gate based on an Indium Phosphide Photonic Crystal Molecule. Wavelength conversion and all-optical mixing of microwave signals are demonstrated using the 2 mW output of a mode locked diode laser. The spectral separation of the optical pump and signal is crucial in suppressing optical cross-talk.

  2. Optical induction of muscle contraction at the tissue scale through intrinsic cellular amplifiers.

    PubMed

    Yoon, Jonghee; Choi, Myunghwan; Ku, Taeyun; Choi, Won Jong; Choi, Chulhee

    2014-08-01

    The smooth muscle cell is the principal component responsible for involuntary control of visceral organs, including vascular tonicity, secretion, and sphincter regulation. It is known that the neurotransmitters released from nerve endings increase the intracellular Ca(2+) level in smooth muscle cells followed by muscle contraction. We herein report that femtosecond laser pulses focused on the diffraction-limited volume can induce intracellular Ca(2+) increases in the irradiated smooth muscle cell without neurotransmitters, and locally increased intracellular Ca(2+) levels are amplified by calcium-induced calcium-releasing mechanisms through the ryanodine receptor, a Ca(2+) channel of the endoplasmic reticulum. The laser-induced Ca(2+) increases propagate to adjacent cells through gap junctions. Thus, ultrashort-pulsed lasers can induce smooth muscle contraction by controlling Ca(2+), even with optical stimulation of the diffraction-limited volume. This optical method, which leads to reversible and reproducible muscle contraction, can be used in research into muscle dynamics, neuromuscular disease treatment, and nanorobot control. PMID:23650149

  3. Simplified optical fiber RGB system in evaluating intrinsic quality of Sala mango

    NASA Astrophysics Data System (ADS)

    Yahaya, Ommi Kalsom Mardziah; Jafri, Mohd. Zubir Mat; Aziz, Azlan Abdul; Omar, Ahmad Fairuz

    2015-06-01

    This study presents an alternative approach for the nondestructive assessment of fruit quality parameters with the use of a simplified optical fiber red-green-blue system (OF-RGB). The optical sensor system presented in this work is designed to rapidly measure the firmness, acidity, and soluble solid content of an intact Sala mango on the basis of color properties. The system consists of three light-emitting diodes with peak emission at 635 (red), 525 (green), and 470 nm (blue), as well as a single photodetector capable of sensing visible light. The measurements were conducted using the reflectance technique. The analyses were conducted by comparing the results obtained through the proposed system with those measured using two commercial spectrometers, namely, QE65000 and FieldSpec 3. The developed RGB system showed satisfactory accuracy in the measurement of acidity (R2=0.795) and firmness (R2=0.761), but a relatively lower accuracy in the measurement of soluble solid content (R2=0.593) of intact mangoes. The results obtained through OF-RGB are comparable with those measured by QE65000 and FieldSpec 3. This system is a promising new technology with rapid response, easy operation, and low cost with potential applications in the nondestructive assessment of quality attributes.

  4. Correlating optical damage threshold with intrinsic defect populations in fused silica as a function of heat treatment temperature

    SciTech Connect

    Shen, N.; Matthews, M. J.; Elhadj, S.; Miller, P. E.; Nelson, A. J.; Hamilton, J.

    2013-04-03

    Here, chemical vapor deposition (CVD) is used for the production of fused silica optics in high-power laser applications. However, relatively little is known about the ultraviolet laser damage threshold of CVD films and how they relate to intrinsic defects produced during deposition. We present here a study relating structural and electronic defects in CVD films to 355 nm pulsed-laser damage threshold as a function of post-deposition annealing temperature (THT). Plasma-enhanced CVD based on SiH4/N2O under oxygen-rich conditions was used to deposit 1.5, 3.1 and 6.4 µm thick films on etched SiO2 substrates. Rapid annealing was performed using a scanned CO2 laser beam up to THT ~ 2100 K. The films were then characterized using x-ray photoemission spectroscopy, Fourier transform infrared spectroscopy (FTIR) and photoluminescence spectroscopy. A gradual transition in the damage threshold of annealed films was observed for THT values up to 1600 K, correlating with a decrease in non-bridging silanol and oxygen deficient centres. An additional sharp transition in damage threshold also occurs at ~1850 K indicating substrate annealing. Based on our results, a mechanism for damage-related defect annealing is proposed, and the potential of using high-THT CVD SiO2 to mitigate optical damage is also discussed.

  5. Quasiparticle Representation of Coherent Nonlinear Optical Signals of Multiexcitons

    NASA Astrophysics Data System (ADS)

    Fingerhut, Benjamin; Bennet, Kochise; Roslyak, Oleksiy; Mukamel, Shaul

    2013-03-01

    Elementary excitations of many-Fermion systems can be described within the quasiparticle approach which is widely used in the calculation of transport and optical properties of metals, semiconductors, molecular aggregates and strongly correlated quantum materials. The excitations are then viewed as independent harmonic oscillators where the many-body interactions between the oscillators are mapped into anharmonicities. We present a Green's function approach based on coboson algebra for calculating nonlinear optical signals and apply it onwards the study of two and three exciton states. The method only requires the diagonalization of the single exciton manifold and avoids equations of motion of multi-exciton manifolds. Using coboson algebra many body effects are recast in terms of tetradic exciton-exciton interactions: Coulomb scattering and Pauli exchange. The physical space of Fermions is recovered by singular-value decomposition of the over-complete coboson basis set. The approach is used to calculate third and fifth order quantum coherence optical signals that directly probe correlations in two- and three exciton states and their projections on the two and single exciton manifold.

  6. A dual-detector optical receiver for PDM signals detection

    NASA Astrophysics Data System (ADS)

    Chen, Guanyu; Yu, Yu; Zhang, Xinliang

    2016-05-01

    We propose and fabricate a silicon based dual-detector optical receiver, which consists of a two dimensional (2D) grating coupler (GC) and two separate germanium photodetectors (Ge PDs). The 2D GC performs polarization diversity, and thus demultiplexing and detection for polarization division multiplexed (PDM) signals can be achieved. Through a specific design with double-sides illumination, the space charge density can be reduced and the responsivity and saturation power can be improved significantly. The measured dark current, responsivity and bandwidth are 0.86 μA, 1.06 A/W and 36 GHz under 3 V reverse biased voltage, respectively. Both DC currents and eye diagrams are measured for the proposed device and the results validate its performance successfully. The power penalty between the single and dual polarized signals is about 1.9 dB under 10 and 20 Gb/s cases for both the two Ge PDs. The proposed direct detection (DD) for PDM signals with high speed, high responsivity and large saturation power is cost-effective and promising for short reach optical communication.

  7. A dual-detector optical receiver for PDM signals detection.

    PubMed

    Chen, Guanyu; Yu, Yu; Zhang, Xinliang

    2016-05-20

    We propose and fabricate a silicon based dual-detector optical receiver, which consists of a two dimensional (2D) grating coupler (GC) and two separate germanium photodetectors (Ge PDs). The 2D GC performs polarization diversity, and thus demultiplexing and detection for polarization division multiplexed (PDM) signals can be achieved. Through a specific design with double-sides illumination, the space charge density can be reduced and the responsivity and saturation power can be improved significantly. The measured dark current, responsivity and bandwidth are 0.86 μA, 1.06 A/W and 36 GHz under 3 V reverse biased voltage, respectively. Both DC currents and eye diagrams are measured for the proposed device and the results validate its performance successfully. The power penalty between the single and dual polarized signals is about 1.9 dB under 10 and 20 Gb/s cases for both the two Ge PDs. The proposed direct detection (DD) for PDM signals with high speed, high responsivity and large saturation power is cost-effective and promising for short reach optical communication.

  8. A dual-detector optical receiver for PDM signals detection

    PubMed Central

    Chen, Guanyu; Yu, Yu; Zhang, Xinliang

    2016-01-01

    We propose and fabricate a silicon based dual-detector optical receiver, which consists of a two dimensional (2D) grating coupler (GC) and two separate germanium photodetectors (Ge PDs). The 2D GC performs polarization diversity, and thus demultiplexing and detection for polarization division multiplexed (PDM) signals can be achieved. Through a specific design with double-sides illumination, the space charge density can be reduced and the responsivity and saturation power can be improved significantly. The measured dark current, responsivity and bandwidth are 0.86 μA, 1.06 A/W and 36 GHz under 3 V reverse biased voltage, respectively. Both DC currents and eye diagrams are measured for the proposed device and the results validate its performance successfully. The power penalty between the single and dual polarized signals is about 1.9 dB under 10 and 20 Gb/s cases for both the two Ge PDs. The proposed direct detection (DD) for PDM signals with high speed, high responsivity and large saturation power is cost-effective and promising for short reach optical communication. PMID:27198501

  9. Guest Editorial: Two-Dimensional Optical Signal Processing

    NASA Astrophysics Data System (ADS)

    Kooij, Theo; Ludman, Jacques E.; Stilwell, P. D., Jr.

    1982-10-01

    When some optical processing systems firms proposed to the Defense Advanced Research Project Agency (DARPA) and the U.S. Navy some years ago that they could beat the ILLIAC-IV-that venerable supercomputer, which until recently was the world's largest by at least a factor of 100, it sounded too good to be true. But they were right, and they did not even have to try hard. The problem was a two-dimensional (2-D) processing task of generating ambiguity surfaces to test whether two received signals came from a common origin, with unknown time and Doppler shifts. The ILLIAC, going all out as an in-line processor for the Acoustic Research Center near San Francisco, California, could just make a handful of such surfaces per second; the optical processors made hundreds, literally sucking their digital inputs dry.

  10. Processing of laser Doppler flowmetry signals from healthy subjects and patients with varicose veins: Information categorisation approach based on intrinsic mode functions and entropy computation.

    PubMed

    Humeau-Heurtier, Anne; Klonizakis, Markos

    2015-06-01

    The diagnosis of pathologies from signal processing approaches has shown to be of importance. This can provide noninvasive information at the earliest stage. In this work, the problem of categorising - in a quantifiable manner - information content of microvascular blood flow signals recorded in healthy participants and patients with varicose veins is addressed. For this purpose, laser Doppler flowmetry (LDF) signals - that reflect microvascular blood flow - recorded both at rest and after acetylcholine (ACh) stimulation (an endothelial-dependent vasodilator) are analyzed. Each signal is processed with the empirical mode decomposition (EMD) to obtain its intrinsic mode functions (IMFs). An entropy measure of each IMFs is then computed. The results show that IMFs of LDF signals have different complexity for different physiologic/pathological states. This is true both at rest and after ACh stimulation. Thus, the proposed framework (EMD + entropy computation) may be used to gain a noninvasive understanding of LDF signals in patients with microvascular dysfunctions.

  11. Detection, Evaluation, and Optimization of Optical Signals Generated by Fiber Optic Bragg Gratings Under Dynamic Excitations

    NASA Technical Reports Server (NTRS)

    Adamovsky, Grigory; Lekki, John; Lock, James A.

    2002-01-01

    The dynamic response of a fiber optic Bragg grating to mechanical vibrations is examined both theoretically and experimentally. The theoretical expressions describing the consequences of changes in the grating's reflection spectrum are derived for partially coherent beams in an interferometer. The analysis is given in terms of the dominant wavelength, optical bandwidth, and optical path difference of the interfering signals. Changes in the reflection spectrum caused by a periodic stretching and compression of the grating were experimentally measured using an unbalanced Michelson interferometer, a Michelson interferometer with a non-zero optical path difference. The interferometer's sensitivity to changes in dominant wavelength of the interfering beams was measured as a function of interferometer unbalance and was compared to theoretical predictions. The theoretical analysis enables the user to determine the optimum performance for an unbalanced interferometer.

  12. Membrane binding mode of intrinsically disordered cytoplasmic domains of T cell receptor signaling subunits depends on lipid composition

    SciTech Connect

    Sigalov, Alexander B.; Hendricks, Gregory M.

    2009-11-13

    Intrinsically disordered cytoplasmic domains of T cell receptor (TCR) signaling subunits including {zeta}{sub cyt} and CD3{epsilon}{sub cyt} all contain one or more copies of an immunoreceptor tyrosine-based activation motif (ITAM), tyrosine residues of which are phosphorylated upon receptor triggering. Membrane binding-induced helical folding of {zeta}{sub cyt} and CD3{epsilon}{sub cyt} ITAMs is thought to control TCR activation. However, the question whether or not lipid binding of {zeta}{sub cyt} and CD3{epsilon}{sub cyt} is necessarily accompanied by a folding transition of ITAMs remains open. In this study, we investigate whether the membrane binding mechanisms of {zeta}{sub cyt} and CD3{epsilon}{sub cyt} depend on the membrane model used. Circular dichroic and fluorescence data indicate that binding of {zeta}{sub cyt} and CD3{epsilon}{sub cyt} to detergent micelles and unstable vesicles is accompanied by a disorder-to-order transition, whereas upon binding to stable vesicles these proteins remain unfolded. Using electron microscopy and dynamic light scattering, we show that upon protein binding, unstable vesicles fuse and rupture. In contrast, stable vesicles remain intact under these conditions. This suggests different membrane binding modes for {zeta}{sub cyt} and CD3{epsilon}{sub cyt} depending on the bilayer stability: (1) coupled binding and folding, and (2) binding without folding. These findings explain the long-standing puzzle in the literature and highlight the importance of the choice of an appropriate membrane model for protein-lipid interactions studies.

  13. Interferometer fiber optic sensor for dc signal measurement

    NASA Astrophysics Data System (ADS)

    Nakajima, Yasuyuki; Sato, Ryotaku; Yoshikawa, T.; Dobashi, Koji

    2001-05-01

    We present a novel interferometer fiber optic sensor (IFOS) system for DC signal measurement based on the PGC (phase generated carrier) demodulation scheme. In addition to the principle of the demodulation, the compensation techniques for improving the measurement precision constrained by the laser nonlinearity in frequency modulation (FM) operation and the experimental results are described. And now, we've made a prototype water level sensor and been trying the field tests, so we show the first results compared with those of the float-typed reference sensor.

  14. Symbol rate identification for auxiliary amplitude modulation optical signal

    NASA Astrophysics Data System (ADS)

    Wei, Junyu; Dong, Zhi; Huang, Zhiping; Zhang, Yimeng

    2016-09-01

    In this paper, we creatively propose and demonstrate a method for symbol rate identification (SRI) of auxiliary amplitude modulation (AAM) optical signal based on asynchronous delay-tap sampling (ADTS) and average magnitude difference function (AMDF). The method can accurately estimate symbol rate and has large transmission impairments tolerance. Furthermore, it can be realized in the digital signal processor (DSP) with low logical resources because of multiplication-free. In order to improve the accuracy of SRI, the peak to valley ratio (PTVR) of AMDF is introduced into our method for blind chromatic dispersion (CD) compensation. The results of the numerical simulations show that the overall maximum SRI error is smaller 0.079% for return-to-zero (RZ) on-off keying (OOK), RZ differential phase-shift keying (DPSK), RZ differential quadrature phase-shift keying (DQPSK) and RZ 16-ary quadrature amplitude modulation (QAM) with 50% duty cycles.

  15. Signaling of the strongest stimulus in the owl optic tectum.

    PubMed

    Mysore, Shreesh P; Asadollahi, Ali; Knudsen, Eric I

    2011-04-01

    Essential to the selection of the next target for gaze or attention is the ability to compare the strengths of multiple competing stimuli (bottom-up information) and to signal the strongest one. Although the optic tectum (OT) has been causally implicated in stimulus selection, how it computes the strongest stimulus is unknown. Here, we demonstrate that OT neurons in the barn owl systematically encode the relative strengths of simultaneously occurring stimuli independently of sensory modality. Moreover, special "switch-like" responses of a subset of neurons abruptly increase when the stimulus inside their receptive field becomes the strongest one. Such responses are not predicted by responses to single stimuli and, indeed, are eliminated in the absence of competitive interactions. We demonstrate that this sensory transformation substantially boosts the representation of the strongest stimulus by creating a binary discrimination signal, thereby setting the stage for potential winner-take-all target selection for gaze and attention. PMID:21471353

  16. Optical modulation techniques for analog signal processing and CMOS compatible electro-optic modulation

    NASA Astrophysics Data System (ADS)

    Gill, Douglas M.; Rasras, Mahmoud; Tu, Kun-Yii; Chen, Young-Kai; White, Alice E.; Patel, Sanjay S.; Carothers, Daniel; Pomerene, Andrew; Kamocsai, Robert; Beattie, James; Kopa, Anthony; Apsel, Alyssa; Beals, Mark; Mitchel, Jurgen; Liu, Jifeng; Kimerling, Lionel C.

    2008-02-01

    Integrating electronic and photonic functions onto a single silicon-based chip using techniques compatible with mass-production CMOS electronics will enable new design paradigms for existing system architectures and open new opportunities for electro-optic applications with the potential to dramatically change the management, cost, footprint, weight, and power consumption of today's communication systems. While broadband analog system applications represent a smaller volume market than that for digital data transmission, there are significant deployments of analog electro-optic systems for commercial and military applications. Broadband linear modulation is a critical building block in optical analog signal processing and also could have significant applications in digital communication systems. Recently, broadband electro-optic modulators on a silicon platform have been demonstrated based on the plasma dispersion effect. The use of the plasma dispersion effect within a CMOS compatible waveguide creates new challenges and opportunities for analog signal processing since the index and propagation loss change within the waveguide during modulation. We will review the current status of silicon-based electrooptic modulators and also linearization techniques for optical modulation.

  17. Hybrid optical/electronic signal processor for laser radar signals in fire control systems

    NASA Astrophysics Data System (ADS)

    Findley, George B., Jr.; Anderson, Christopher S.; Townley, S. K.; Pascale, Michael J.; Watson, Lee V.; Jenkinson, Howard A.

    1992-07-01

    This paper reports on the development of a hybrid optical/electronic signal processor for laser radar signals in fire control applications. The breadboard system being developed consists of three subsystems: (1) a signal generator producing target-representative signals, (2) the signal processor consisting of a radiometric channel and a Doppler channel, and (3) a data acquisition, analysis, and display subsystem. The radiometric channel provides target ladar cross section (LCS) resolved in crossrange, while the Doppler channel provides target radial velocity, also resolved in crossrange. Data from the two channels is fused and processed within the data analysis subsystem. Results are to be displayed in near real-time. The breadboard system will be used to demonstrate the capabilities of hybrid signal processor technology and to investigate processing laser radar returns for noncooperative target recognition, target orientation determination, and target trajectory estimation functions. It is anticipated that these functions will enhance the effectiveness of advanced fire control systems in future helicopters and ground vehicles.

  18. Phase resolved digital signal processing in optical coherence tomography

    NASA Astrophysics Data System (ADS)

    de Boer, Johannes F.; Tripathi, Renu; Park, Boris H.; Nassif, Nader

    2002-06-01

    We present phase resolved digital signal processing techniques for Optical Coherence Tomography to correct for the non Gaussian shape of source spectra and for Group Delay Dispersion (GDD). A broadband source centered at 820 nm was synthesized by combining the spectra of two superluminescent diodes to improve axial image resolution in an optical coherence tomography (OCT) system. Spectral shaping was used to reduce the side lobes (ringing) in the axial point spread function due to the non-Gaussian shape of the spectra. Images of onion cells taken with each individual source and the combined sources, respectively, show the improved resolution and quality enhancement in a turbid biological sample. An OCT system operating at 1310 nm was used to demonstrate that the broadening effect of group delay dispersion (GDD) on the coherence function could be eliminated completely by introducing a quadratic phase shift in the Fourier domain of the interferometric signal. The technique is demonstrated by images of human skin grafts with group delay dispersion mismatch between sample and reference arm before and after digital processing.

  19. Time-domain model of quantum-dot semiconductor optical amplifiers for wideband optical signals.

    PubMed

    Puris, D; Schmidt-Langhorst, C; Lüdge, K; Majer, N; Schöll, E; Petermann, K

    2012-11-19

    We present a novel theoretical time-domain model for a quantum dot semiconductor optical amplifier, that allows to simulate subpicosecond pulse propagation including power-based and phase-based effects. Static results including amplified spontaneous emission spectra, continuous wave amplification, and four-wave mixing experiments in addition to dynamic pump-probe simulations are presented for different injection currents. The model uses digital filters to describe the frequency dependent gain and microscopically calculated carrier-carrier scattering rates for the interband carrier dynamics. It can be used to calculate the propagation of multiple signals with different wavelengths or one wideband signal with high bitrate.

  20. Broken Optical Symmetry in DNA-SWNT Hybrids: Spectroscopic Signaling of the Helical Wrap

    NASA Astrophysics Data System (ADS)

    Rotkin, Slava V.

    2009-03-01

    Functionalizing single-stranded DNA on a single-wall carbon nanotube (SWNT) has allowed isolating individual tubes, making them soluble, and separating SWNTs according to their chirality. Such strong technological impact motivated our study of the optical properties of the DNA-SWNT hybrids, commonly used now for the solution-based fabrication and experiments. The helicity of the DNA wrap may interfere with the intrinsic Hamiltonian of the SWNT and result in bandstructure modulation. Our modeling predicts a symmetry lowering in the hybrid due to the Coulomb potential of the regular helical wrap of the ionized backbone of the ssDNA, followed by the qualitative changes in the cross- or circularly polarized SWNT absorption spectrum (with no or little change in the parallel polarization). In particular, we predict the appearance of a new peak in the cross-polarized absorption of the ssDNA-SWNT at a frequency lower than that of all allowed transitions in the bare tube. Such effect can be used for optical identification of the wrap at sufficient ssDNA coverage. Wrap signaling happens also via another optical effect, a strong circular dichroism even in the complex with an achiral SWNT, and even at the frequencies where ss-DNA has no absorption features at all. Symmetry of the wrap is central to determine such a circular dichroism of the hybrid. Having in mind that the exact geometry of a DNA wrap for an arbitrary tube is not precisely known yet, we put forward a general model capable of tracking optical effects, varying the parameters of the wrap and/or tube diameter. For various ssDNA backbone helical angles and for various tubes we predict different absorption spectra, though a general qualitative feature of the helical symmetry breaking, the appearance of new van Hove singularities and circular dichroism, must be present.

  1. Fiber-optic delay-line stabilization of heterodyne optical signal generator and method using same

    NASA Technical Reports Server (NTRS)

    Logan, Ronald T. (Inventor)

    1997-01-01

    The present invention is a laser heterodyne frequency generator system with a stabilizer for use in the microwave and millimeter-wave frequency ranges utilizing a photonic mixer as a photonic phase detector in a stable optical fiber delay-line. Phase and frequency fluctuations of the heterodyne laser signal generators are stabilized at microwave and millimeter wave frequencies by a delay line system operating as a frequency discriminator. The present invention is free from amplifier and mixer 1/.function. noise at microwave and millimeter-wave frequencies that typically limit phase noise performance in electronic cavity stabilized electronic oscillators. Thus, 1/.function. noise due to conventional mixers is eliminated and stable optical heterodyne generation of electrical signals is achieved.

  2. Wide-band analog frequency modulation of optic signals using indirect techniques

    NASA Technical Reports Server (NTRS)

    Fitzmartin, D. J.; Balboni, E. J.; Gels, R. G.

    1991-01-01

    The wideband frequency modulation (FM) of an optical carrier by a radio frequency (RF) or microwave signal can be accomplished independent of laser type when indirect modulation is employed. Indirect modulators exploit the integral relation of phase to frequency so that phase modulators can be used to impress frequency modulation on an optical carrier. The use of integrated optics phase modulators, which are highly linear, enables the generation of optical wideband FM signals with very low intermodulation distortion. This modulator can be used as part of an optical wideband FM link for RF and microwave signals. Experimental results from the test of an indirect frequency modulator for an optical carrier are discussed.

  3. AM-FM analysis of optical coherence tomography signals

    NASA Astrophysics Data System (ADS)

    Kartakoulis, Andreas; Bousi, Evgenia; Pitris, Costas

    2009-02-01

    The early stages of malignant diseases, such as cancer, are characterized by cellular and microstructural changes which define both the diagnosis and the prognosis of the disease. Unfortunately, at the current resolution of Optical Coherence Tomography (OCT), such changes associated with early cancer are not clearly discernible. However, spectral analysis of OCT images has recently shown that additional information can be extracted from those signals, resulting in improved contrast which is directly related to scatterer size changes. Amplitude Modulation - Frequency Modulation (AM-FM) analysis is a fast and accurate technique which can also be applied to the OCT images for estimation of spectral information. It is based on the analytic signal of the real data, obtained using a Hilbert Transform, and provides the instantaneous amplitude, phase, and frequency of an OCT signal. The performance of this method is superior to both FFT-based and parametric (e.g. autoregressive) spectral analysis providing better accuracy and faster convergence when estimating scatterer features. Since disease tissues exhibit variations in scatterer size and thus also exhibit marked differences in spectral and phase characteristics, such advanced analysis techniques can provide more insight into the subtle changes observed in OCT images of malignancy. Therefore, they can make available a tool which could prove extremely valuable for the investigation of disease features which now remain below the resolution of OCT and improved the technology's diagnostic capabilities.

  4. Fiber optic signal amplifier using thermoelectric power generation

    DOEpatents

    Hart, M.M.

    1995-04-18

    A remote fiber optic signal amplifier for use as a repeater/amplifier, such as in transoceanic communications, powered by a Pu{sub 238} or Sr{sub 90} thermoelectric generator. The amplifier comprises a unit with connections on the receiving and sending sides of the communications system, and an erbium-doped fiber amplifier connecting each sending fiber to each receiving fiber. The thermoelectric generator, preferably a Pu{sub 238} or Sr{sub 90} thermoelectric generator delivers power to the amplifiers through a regulator. The heat exchange surfaces of the thermoelectric generator are made of materials resistant to corrosion and biological growth and are directly exposed to the outside, such as the ocean water in transoceanic communications. 2 figs.

  5. Fiber optic signal amplifier using thermoelectric power generation

    DOEpatents

    Hart, Mark M.

    1995-01-01

    A remote fiber optic signal amplifier for use as a repeater/amplifier, such as in transoceanic communications, powered by a Pu.sub.238 or Sr.sub.90 thermoelectric generator. The amplifier comprises a unit with connections on the receiving and sending sides of the communications system, and an erbium-doped fiber amplifier connecting each sending fiber to each receiving fiber. The thermoelectric generator, preferably a Pu.sub.238 or Sr.sub.90 thermoelectric generator delivers power to the amplifiers through a regulator. The heat exchange surfaces of the thermoelectric generator are made of materials resistant to corrosion and biological growth and are directly exposed to the outside, such as the ocean water in transoceanic communications.

  6. Fiber optic signal amplifier using thermoelectric power generation

    DOEpatents

    Hart, M.M.

    1993-01-01

    A remote fiber optic signal amplifier for use as a repeater/amplifier, such as in transoceanic communication, powered by a Pu{sub 238} or Sr{sub 90} thermoelectric generator. The amplifier comprises a unit with connections on the receiving and sending sides of the communications system, and an erbium-doped fiber amplifier connecting each sending fiber to each receiving fiber. The thermoelectric generator, preferably a Pu{sub 238} or Sr{sub 90} thermoelectric generator delivers power to the amplifiers through a regulator. The heat exchange surfaces of the thermoelectric generator are made of material resistant to corrosion and biological growth and are directly exposed to the outside, such as the ocean water in transoceanic communications.

  7. Signal digitizing system and method based on amplitude-to-time optical mapping

    DOEpatents

    Chou, Jason; Bennett, Corey V; Hernandez, Vince

    2015-01-13

    A signal digitizing system and method based on analog-to-time optical mapping, optically maps amplitude information of an analog signal of interest first into wavelength information using an amplitude tunable filter (ATF) to impress spectral changes induced by the amplitude of the analog signal onto a carrier signal, i.e. a train of optical pulses, and next from wavelength information to temporal information using a dispersive element so that temporal information representing the amplitude information is encoded in the time domain in the carrier signal. Optical-to-electrical conversion of the optical pulses into voltage waveforms and subsequently digitizing the voltage waveforms into a digital image enables the temporal information to be resolved and quantized in the time domain. The digital image may them be digital signal processed to digitally reconstruct the analog signal based on the temporal information with high fidelity.

  8. Transmission of multiplexed video signals in multimode optical fiber systems

    NASA Technical Reports Server (NTRS)

    White, Preston, III

    1988-01-01

    Kennedy Space Center has the need for economical transmission of two multiplexed video signals along multimode fiberoptic systems. These systems must span unusual distances and must meet RS-250B short-haul standards after reception. Bandwidth is a major problem and studies of the installed fibers, available LEDs and PINFETs led to the choice of 100 MHz as the upper limit for the system bandwidth. Optical multiplexing and digital transmission were deemed inappropriate. Three electrical multiplexing schemes were chosen for further study. Each of the multiplexing schemes included an FM stage to help meet the stringent S/N specification. Both FM and AM frequency division multiplexing methods were investigated theoretically and these results were validated with laboratory tests. The novel application of quadrature amplitude multiplexing was also considered. Frequency division multiplexing of two wideband FM video signal appears the most promising scheme although this application requires high power highly linear LED transmitters. Futher studies are necessary to determine if LEDs of appropriate quality exist and to better quantify performance of QAM in this application.

  9. Iridescent flowers? Contribution of surface structures to optical signaling.

    PubMed

    van der Kooi, Casper J; Wilts, Bodo D; Leertouwer, Hein L; Staal, Marten; Elzenga, J Theo M; Stavenga, Doekele G

    2014-07-01

    The color of natural objects depends on how they are structured and pigmented. In flowers, both the surface structure of the petals and the pigments they contain determine coloration. The aim of the present study was to assess the contribution of structural coloration, including iridescence, to overall floral coloration. We studied the reflection characteristics of flower petals of various plant species with an imaging scatterometer, which allows direct visualization of the angle dependence of the reflected light in the hemisphere above the petal. To separate the light reflected by the flower surface from the light backscattered by the components inside (e.g. the vacuoles), we also investigated surface casts. A survey among angiosperms revealed three different types of floral surface structure, each with distinct reflections. Petals with a smooth and very flat surface had mirror-like reflections and petal surfaces with cones yielded diffuse reflections. Petals with striations yielded diffraction patterns when single cells were illuminated. The iridescent signal, however, vanished when illumination similar to that found in natural conditions was applied. Pigmentary rather than structural coloration determines the optical appearance of flowers. Therefore, the hypothesized signaling by flowers with striated surfaces to attract potential pollinators presently seems untenable.

  10. Iridescent flowers? Contribution of surface structures to optical signaling.

    PubMed

    van der Kooi, Casper J; Wilts, Bodo D; Leertouwer, Hein L; Staal, Marten; Elzenga, J Theo M; Stavenga, Doekele G

    2014-07-01

    The color of natural objects depends on how they are structured and pigmented. In flowers, both the surface structure of the petals and the pigments they contain determine coloration. The aim of the present study was to assess the contribution of structural coloration, including iridescence, to overall floral coloration. We studied the reflection characteristics of flower petals of various plant species with an imaging scatterometer, which allows direct visualization of the angle dependence of the reflected light in the hemisphere above the petal. To separate the light reflected by the flower surface from the light backscattered by the components inside (e.g. the vacuoles), we also investigated surface casts. A survey among angiosperms revealed three different types of floral surface structure, each with distinct reflections. Petals with a smooth and very flat surface had mirror-like reflections and petal surfaces with cones yielded diffuse reflections. Petals with striations yielded diffraction patterns when single cells were illuminated. The iridescent signal, however, vanished when illumination similar to that found in natural conditions was applied. Pigmentary rather than structural coloration determines the optical appearance of flowers. Therefore, the hypothesized signaling by flowers with striated surfaces to attract potential pollinators presently seems untenable. PMID:24713039

  11. Dietary chlorophyllin inhibits the canonical NF-κB signaling pathway and induces intrinsic apoptosis in a hamster model of oral oncogenesis.

    PubMed

    Thiyagarajan, P; Senthil Murugan, R; Kavitha, K; Anitha, P; Prathiba, D; Nagini, S

    2012-03-01

    Chlorophyllin, a water-soluble, semi-synthetic derivative of the ubiquitous green pigment chlorophyll is shown to exert potent anticarcinogenic effects. In the present study, we investigated the chemopreventive effects of chlorophyllin on 7,12-dimethylbenz(a)anthracene (DMBA)-induced hamster buccal pouch (HBP) carcinogenesis by analyzing the expression of NF-κB family members and markers of intrinsic apoptosis. Dietary administration of chlorophyllin (4 mg/kg bw) suppressed the development of HBP carcinomas by inhibiting the canonical NF-κB signaling pathway by downregulating IKKβ, preventing the phosphorylation of IκB-α, and reducing the expression of nuclear NF-κB. Inactivation of NF-κB signaling by chlorophyllin was associated with the induction of intrinsic apoptosis as evidenced by modulation of Bcl-2 family proteins, enforced nuclear localization of survivin, upregulation of apoptogenic molecules, activation of caspases, and cleavage of PARP. The results of the present study demonstrate that chlorophyllin inhibits the development of DMBA-induced HBP carcinogenesis by targeting NF-κB and the intrinsic apoptotic pathway. Thus, dietary agents such as chlorophyllin that simultaneously target divergent pathways of cell survival and cell death are novel candidates for cancer chemoprevention. PMID:22210229

  12. Coherent detection and digital signal processing for fiber optic communications

    NASA Astrophysics Data System (ADS)

    Ip, Ezra

    The drive towards higher spectral efficiency in optical fiber systems has generated renewed interest in coherent detection. We review different detection methods, including noncoherent, differentially coherent, and coherent detection, as well as hybrid detection methods. We compare the modulation methods that are enabled and their respective performances in a linear regime. An important system parameter is the number of degrees of freedom (DOF) utilized in transmission. Polarization-multiplexed quadrature-amplitude modulation maximizes spectral efficiency and power efficiency as it uses all four available DOF contained in the two field quadratures in the two polarizations. Dual-polarization homodyne or heterodyne downconversion are linear processes that can fully recover the received signal field in these four DOF. When downconverted signals are sampled at the Nyquist rate, compensation of transmission impairments can be performed using digital signal processing (DSP). Software based receivers benefit from the robustness of DSP, flexibility in design, and ease of adaptation to time-varying channels. Linear impairments, including chromatic dispersion (CD) and polarization-mode dispersion (PMD), can be compensated quasi-exactly using finite impulse response filters. In practical systems, sampling the received signal at 3/2 times the symbol rate is sufficient to enable an arbitrary amount of CD and PMD to be compensated for a sufficiently long equalizer whose tap length scales linearly with transmission distance. Depending on the transmitted constellation and the target bit error rate, the analog-to-digital converter (ADC) should have around 5 to 6 bits of resolution. Digital coherent receivers are naturally suited for the implementation of feedforward carrier recovery, which has superior linewidth tolerance than phase-locked loops, and does not suffer from feedback delay constraints. Differential bit encoding can be used to prevent catastrophic receiver failure due

  13. Intrinsic MyD88-Akt1-mTOR Signaling Coordinates Disparate Tc17 and Tc1 Responses during Vaccine Immunity against Fungal Pneumonia.

    PubMed

    Nanjappa, Som Gowda; Hernández-Santos, Nydiaris; Galles, Kevin; Wüthrich, Marcel; Suresh, M; Klein, Bruce S

    2015-09-01

    Fungal infections have skyrocketed in immune-compromised patients lacking CD4+ T cells, underscoring the need for vaccine prevention. An understanding of the elements that promote vaccine immunity in this setting is essential. We previously demonstrated that vaccine-induced IL-17A+ CD8+ T cells (Tc17) are required for resistance against lethal fungal pneumonia in CD4+ T cell-deficient hosts, whereas the individual type I cytokines IFN-γ, TNF-α and GM-CSF, are dispensable. Here, we report that T cell-intrinsic MyD88 signals are crucial for these Tc17 cell responses and vaccine immunity against lethal fungal pneumonia in mice. In contrast, IFN-γ+ CD8+ cell (Tc1) responses are largely normal in the absence of intrinsic MyD88 signaling in CD8+ T cells. The poor accumulation of MyD88-deficient Tc17 cells was not linked to an early onset of contraction, nor to accelerated cell death or diminished expression of anti-apoptotic molecules Bcl-2 or Bcl-xL. Instead, intrinsic MyD88 was required to sustain the proliferation of Tc17 cells through the activation of mTOR via Akt1. Moreover, intrinsic IL-1R and TLR2, but not IL-18R, were required for MyD88 dependent Tc17 responses. Our data identify unappreciated targets for augmenting adaptive immunity against fungi. Our findings have implications for designing fungal vaccines and immune-based therapies in immune-compromised patients.

  14. Optical signal processing for enabling high-speed, highly spectrally efficient and high capacity optical systems

    NASA Astrophysics Data System (ADS)

    Fazal, Muhammad Irfan

    may be possible. Recently, interest has increased in exploring the spatial dimension of light to increase capacity, both in fiber as well as free-space communication channels. The orbital angular momentum (OAM) of light, carried by Laguerre-Gaussian (LG) beams have the interesting property that, in theory, an infinite number of OAMs can be transmitted; which due to its inherent orthogonality will not affect each other. Thus, in theory, one can increase the channel capacity arbitrarily. However, in practice, the device dimensions will reduce the number of OAMs used. In addition to advanced modulation formats, it is expected that optical signal processing may play a role in the future development of more efficient optical transmission systems. The hope is that performing signal processing in the optical domain may reduce optical-to-electronic conversion inefficiencies, eliminate bottlenecks and take advantage of the ultrahigh bandwidth inherent in optics. While 40 to 50 Gbit/s electronic components are the peak of commercial technology and 100 Gbit/s capable RF components are still in their infancy, optical signal processing of these high-speed data signals may provide a potential solution. Furthermore, any optical processing system or sub-system must be capable of handling the wide array of data formats and data rates that networks may employ. The work presented in this Ph.D. dissertation attempts at addressing the issue of optical processing for advanced optical modulation formats, and particularly explores the state of the art in increasing the capacity of an optical link by a combination of wavelength/phase/polarization/OAM dimensions of light. Spatial multiplexing and demultiplexing of both coherently and directly detected signals at the 100 Gbit/s Ethernet standard is addressed. The application of a continuously tunable all-optical delay for all-optical functionality like time-slot interchange at high data-rates is presented. Moreover the interplay of chirp

  15. Spectral and spatial dependence of
diffuse optical signals in response to
peripheral nerve stimulation

    PubMed Central

    Chen, Debbie K.; Erb, M. Kelley; Tong, Yunjie; Yu, Yang; Sassaroli, Angelo; Bergethon, Peter R.; Fantini, Sergio

    2010-01-01

    Using non-invasive, near-infrared spectroscopy we have previously reported optical signals measured at or around peripheral nerves in response to their stimulation. Such optical signals featured amplitudes on the order of 0.1% and peaked about 100 ms after peripheral nerve stimulation in human subjects. Here, we report a study of the spatial and spectral dependence of the optical signals induced by stimulation of the human median and sural nerves, and observe that these optical signals are: (1) unlikely due to either dilation or constriction of blood vessels, (2) not associated with capillary bed hemoglobin, (3) likely due to blood vessel(s) displacement, and (4) unlikely due to fiber-skin optical coupling effects. We conclude that the most probable origin of the optical response to peripheral nerve stimulation is from displacement of blood vessels within the optically probed volume, as a result of muscle twitch in adjacent areas. PMID:21258519

  16. Photonic devices and systems for optical signal processing

    NASA Astrophysics Data System (ADS)

    Parker, Michael A.; Swanson, Paul D.; Libby, Stuart I.

    1993-08-01

    This report is a compilation of the preliminary designs, fabrication, and test results for multiquantum well optical amplifiers, stripe and square broad area lasers, mode switched optical memory elements, optical RS flip flops, NOR gates, photodetectors, and acousto-optic switches.

  17. Propolis cinnamic acid derivatives induce apoptosis through both extrinsic and intrinsic apoptosis signaling pathways and modulate of miRNA expression.

    PubMed

    Kumazaki, Minami; Shinohara, Haruka; Taniguchi, Kohei; Yamada, Nami; Ohta, Shozo; Ichihara, Kenji; Akao, Yukihiro

    2014-01-01

    Propolis cinnamic acid derivatives have a number of biological activities including anti-oxidant and anti-cancer ones. In this study, we aimed to elucidate the mechanism of the anti-cancer activity of 3 representative propolis cinnamic acid derivatives, i.e., Artepilin C, Baccharin and Drupanin in human colon cancer cell lines. Our study demonstrated that these compounds had a potent apoptosis-inductive effect even on drug-resistant colon cancer cells. Combination treatment of human colon cancer DLD-1 cells with 2 of these compounds, each at its IC20 concentration, induced apoptosis by stimulating both intrinsic and extrinsic apoptosis signaling pathways. Especially, Baccharin plus Drupanin exhibited a synergistic growth-inhibitory effect by strengthening both intrinsic and extrinsic apoptotic signaling transduction through TRAIL/DR4/5 and/or FasL/Fas death-signaling loops and by increasing the expression level of miR-143, resulting in decreased expression levels of the target gene MAPK/Erk5 and its downstream target c-Myc. These data suggest that the supplemental intake of these compounds found in propolis has enormous significance with respect to cancer prevention.

  18. An additional channel for FM signal transmission in standard fiber-optic AM communication lines

    NASA Astrophysics Data System (ADS)

    Lebedev, V. V.; Anufriev, K. M.; Toguzov, N. V.; Il'ichev, I. V.; Shamray, A. V.

    2015-11-01

    A paradigm has been developed according to which an additional FM signal transfer channel is formed in a standard optical fiber transmission line without violating the main AM channel operation. Using the proposed approach, an RS-232 interface signal has been experimentally transferred via a standard intraobject 100-Mbit Ethernet line based on an SMF-28 single-mode optical fiber.

  19. Coherent (photon) vs incoherent (current) detection of multidimensional optical signals from single molecules in open junctions

    SciTech Connect

    Agarwalla, Bijay Kumar; Hua, Weijie; Zhang, Yu; Mukamel, Shaul; Harbola, Upendra

    2015-06-07

    The nonlinear optical response of a current-carrying single molecule coupled to two metal leads and driven by a sequence of impulsive optical pulses with controllable phases and time delays is calculated. Coherent (stimulated, heterodyne) detection of photons and incoherent detection of the optically induced current are compared. Using a diagrammatic Liouville space superoperator formalism, the signals are recast in terms of molecular correlation functions which are then expanded in the many-body molecular states. Two dimensional signals in benzene-1,4-dithiol molecule show cross peaks involving charged states. The correlation between optical and charge current signal is also observed.

  20. Luteolin induces cell cycle arrest and apoptosis through extrinsic and intrinsic signaling pathways in MCF-7 breast cancer cells.

    PubMed

    Park, Su-Ho; Ham, Sunyoung; Kwon, Tae Ho; Kim, Man Sub; Lee, Dong Hun; Kang, Jeoung-Woo; Oh, Sei-Ryang; Yoon, Do-Young

    2014-01-01

    Luteolin is a common flavonoid that exists in medicinal herbs, fruits, and vegetables. Luteolin has biochemical functions including anti-allergy, anti-inflammation, and anti-cancer functions. However, its efficacy and precise mode of action against breast cancer are still under study. To elucidate whether luteolin exhibits an anticancer effect in breast cancer, MCF-7 breast cancer cells were incubated with luteolin, and apoptosis was assessed by observing nuclear morphological changes and by performing cell viability assay, cell cycle analysis, annexin V-FITC/PI double staining, western blotting, RT-PCR, and mitochondrial membrane potential measurements. Luteolin inhibited growth through perturbation of cell cycle progression at the sub-G1 and G1 phases in MCF-7 cells. Furthermore, luteolin enhanced the expression of death receptors, such as DR5, and activated caspase cascades. It enhanced the activities of caspase-8/-9/-3 in a dose-dependent manner, followed by inactivation of PARP. Activation of caspase-8 and caspase-9 induced caspase-3 activity, respectively, in apoptosis of extrinsic and intrinsic pathways. Luteolin also induced mitochondrial membrane potential collapse and cytochrome c release, and increased Bax expression by inhibiting expression of Bcl-2. Taken together, these results suggest that luteolin provokes cell cycle arrest and induces apoptosis by activating the extrinsic and intrinsic pathways. PMID:25272060

  1. Simultaneous multiple-depths en-face optical coherence tomography using multiple signal excitation of acousto-optic deflectors.

    PubMed

    Zurauskas, Mantas; Rogers, John; Podoleanu, Adrian Gh

    2013-01-28

    We present a novel low-coherence interferometer configuration, equipped with acousto-optic deflectors that can be used to simultaneously acquire up to eight time domain optical coherence tomography en-face images. The capabilities of the configuration are evaluated in terms of depth resolution, signal to noise ratio and crosstalk. Then the configuration is employed to demonstrate simultaneous en-face optical coherence tomography imaging at five different depths in a specimen of armadillidium vulgare. PMID:23389175

  2. Differential geometry of the ruled surfaces optically generated by mirror-scanning devices. I. Intrinsic and extrinsic properties of the scan field.

    PubMed

    Li, Yajun

    2011-04-01

    Rectilinear propagation of light rays in homogeneous isotropic media makes it possible for optical generation of ruled surfaces as the ray is deflected by a rotatable mirror. Scan patterns on a plane or curved surface are merely curves on the ruled surface. Based on this understanding, structures of the scan fields produced by mirror-scanning devices of different configurations are investigated in terms of differential geometry. Expressions of the first and second fundamental coefficients and the first and second Gauss differential forms are given for an investigation of the intrinsic properties of the optically generated ruled surfaces. The Plücker ruled conoid is then generalized for mathematical modeling of the scan fields produced by single-mirror scanning devices of different configurations. Part II will be devoted to a study of multi-mirror scanning systems for optical generation of well-known ruled surfaces such as helicoids and hyperbolic paraboloids.

  3. Photonic vector signal generation at microwave/millimeter-wave bands employing an optical frequency quadrupling scheme.

    PubMed

    Lin, Chun-Ting; Shih, Po-Tsung; Jiang, Wen-Jr; Wong, Er-Zih; Chen, Jason Jyehong; Chi, Sien

    2009-07-15

    To the best of our knowledge, a novel photonic architecture to generate vector signals at microwave/millimeter-wave bands employing an optical frequency quadrupling technique based on an external dual-parallel modulator is proposed for the first time. A 312.5 MSym/s quadruple phase-shift keying signal at 25 GHz is experimentally demonstrated using properly precoding driving signal at 6.25 GHz, and optical power penalty is negligible following 50 km single-mode fiber transmission.

  4. Optical signal impairment study of cascaded optical filters in 40 Gbps DQPSK and 100 Gbps PM-DQPSK systems

    NASA Astrophysics Data System (ADS)

    Chen, Xiaoyong; Horche, Paloma R.; Minguez, Alfredo M.

    2013-09-01

    Optical filters are crucial elements in optical communications. The influence of cascaded filters in the optical signal will affect the communications quality seriously. In this paper we will study and simulate the optical signal impairment caused by different kinds of filters which include Butterworth, Bessel, Fiber Bragg Grating (FBG) and Fabry-Perot (FP). Optical signal impairment is analyzed from an Eye Opening Penalty (EOP) and optical spectrum point of view. The simulation results show that when the center frequency of all filters aligns with the laser's frequency, the Butterworth has the smallest influence to the signal while the F-P has the biggest. With a -1dB EOP, the amount of cascaded Butterworth optical filters with a bandwidth of 50 GHz is 18 in 40 Gbps NRZ-DQPSK systems and 12 in 100 Gbps PMNRZ- DQPSK systems. The value is reduced to 9 and 6 respectively for Febry-Perot optical filters. In the situation of frequency misalignment, the impairment caused by filters is more serious. Our research shows that with a frequency deviation of 5 GHz, only 12 and 9 Butterworth optical filters can be cascaded in 40 Gbps NRZ-DQPSK and 100 Gbps PM-NRZ-DQPSK systems respectively. We also study the signal impairment caused by different orders of the Butterworth filter model. Our study shows that although the higher-order has a smaller clipping effect in the transmission spectrum, it will introduce a more serious phase ripple which seriously affects the signal. Simulation result shows that the 2nd order Butterworth filter has the best performance.

  5. Intrinsic Properties of immunoglobulin IgG1 Isotype-Switched B Cell Receptors Promote Microclustering and the Initiation of Signaling

    PubMed Central

    Liu, Wanli; Meckel, Tobias; Tolar, Pavel; Sohn, Hae Won; Pierce, Susan K.

    2010-01-01

    Summary Memory B cells express high affinity, immunoglobulin B cell receptors (IgG-BCRs) that enhance B cell responses giving rise to the rapid production of high affinity, IgG antibodies. Despite the central role of IgG-BCRs in memory responses, the mechanisms by which the IgG-BCRs function to enhance B cell responses are not fully understood. Using high-resolution live-cell imaging we showed that independent of affinity, IgG1-BCRs dramatically enhanced the earliest BCR-intrinsic events that followed within seconds of B cells’ encounter with membrane bound antigen including BCR oligomerization and BCR microcluster growth, leading to Syk kinase recruitment and calcium responses. The enhancement of these early events was dependent on a membrane proximal region of the IgG1 cytoplasmic tail not previously appreciated to play a role in IgG1-BCR signaling. Thus, intrinsic properties of the IgG1-BCR enhance early antigen-driven events that ultimately translate into heightened signaling. PMID:20620943

  6. Raldh2 expression in optic vesicle generates a retinoic acid signal needed for invagination of retina during optic cup formation.

    PubMed

    Mic, Felix A; Molotkov, Andrei; Molotkova, Natalia; Duester, Gregg

    2004-10-01

    Three retinaldehyde dehydrogenase genes (Raldh1, Raldh2, and Raldh3) expressed in unique spatiotemporal patterns may control synthesis of retinoic acid (RA) needed for retina development. However, previous studies indicate that retina formation still proceeds normally in Raldh1-/- mouse embryos lacking RA synthesis in the dorsal neural retina at the optic cup stage. Here, we demonstrate that Raldh2-/- embryos lacking RA synthesis in the optic vesicle exhibit a failure in retina invagination needed to develop an optic cup. This was also observed in Raldh1-/-:Raldh2-/- double mutants, which develop similarly. Both mutants retain RA activity in the lens placode associated with Raldh3 expression, but this RA activity is insufficient to induce optic cup formation. Maternal RA administration at the optic vesicle stage rescues optic cup formation in Raldh2-/- and Raldh1-/-:Raldh2-/- embryos, demonstrating that Raldh1 is not required during rescue of optic cup development. The optic cup of rescued Raldh1-/-:Raldh2-/- embryos exhibits normal RA activity and this is associated with Raldh3 expression in the retina and lens. Thus, RA signaling initiates in the optic vesicle in response to Raldh2 but can be maintained during optic cup formation by a gene other than Raldh1, most likely Raldh3. Loss of optic vesicle RA signaling does not effect expression of early determinants of retina at the optic vesicle stage (Pax6, Six3, Rx, Mitf). Our findings suggest that RA functions as one of the signals needed for invagination of the retina to generate an optic cup. PMID:15366004

  7. Silver-Overgrowth-Induced Changes in Intrinsic Optical Properties of Gold Nanorods: From Noninvasive Monitoring of Growth Kinetics to Tailoring Internal Mirror Charges

    PubMed Central

    2015-01-01

    We investigate the effect of surfactant-mediated, asymmetric silver overgrowth of gold nanorods on their intrinsic optical properties. From concentration-dependent experiments, we established a close correlation of the extinction in the UV/vis/NIR frequency range and the morphological transition from gold nanorods to Au@Ag cuboids. Based on this correlation, a generic methodology for in situ monitoring of the evolution of the cuboid morphology was developed and applied in time-dependent experiments. We find that growth rates are sensitive to the substitution of the surfactant headgroup by comparison of benzylhexadecyldimethylammonium chloride (BDAC) with hexadecyltrimethylammonium chloride (CTAC). The time-dependent overgrowth in BDAC proceeds about 1 order of magnitude slower than in CTAC, which allows for higher control during silver overgrowth. Furthermore, silver overgrowth results in a qualitatively novel optical feature: Upon excitation inside the overlap region of the interband transition of gold and intraband of silver, the gold core acts as a retarding element. The much higher damping of the gold core compared to the silver shell in Au@Ag cuboids induces mirror charges at the core/shell interface as shown by electromagnetic simulations. Full control over the kinetic growth process consequently allows for precise tailoring of the resonance wavelengths of both modes. Tailored and asymmetric silver-overgrown gold nanorods are of particular interest for large-scale fabrication of nanoparticles with intrinsic metamaterial properties. These building blocks could furthermore find application in optical sensor technology, light harvesting, and information technology. PMID:26113885

  8. High-Speed Characterizatin of Optical Telecommunication Signals

    SciTech Connect

    Dorrer, C.

    2007-05-17

    Optical telecommunication systems constantly evolve toward higher bit rates, requiring the modulation and detection of higher-bandwidth optical waves. Commercial systems operating at 40 Gb/s are now available and research and development efforts are targeting higher bit rates for which optical pulses with picosecond-range duration are used. Chromatic dispersion, nonlinearities and amplified spontaneous emission from optical amplifiers are sources of transmission impairments that must be characterized and mitigated. Advanced modulation formats rely on the modulation of not only the amplitude of an optical wave (e.g., on/off keying), but also its phase (e.g., phase-shift keying) in order to optimize the transmission capabilities. The importance of the characterization of the properties of optical sources and components and the specificities of the optical telecommunication environment with respect to ultrafast optics are emphasized. Various diagnostics measuring the electric field of optical sources in the telecommunication environment are described. Sampling diagnostics capable of measuring eye diagrams and constellation diagrams of high-bit-rate, data-encoded sources are presented. Various optical pulse characterization techniques that meet the sensitivity requirements imposed by the telecommunication environment are also described.

  9. Multi-band local microwave signal generation based on an optical frequency comb generator

    NASA Astrophysics Data System (ADS)

    Wang, Wen Ting; Liu, Jian Guo; Sun, Wen Hui; Chen, Wei; Zhu, Ning Hua

    2015-03-01

    We propose and experimental demonstrate a new method to generate multi-band local microwave signals based on an optical frequency comb generator (OFCG) by applying an optical sideband injection locking technique and an optical heterodyning technique. The generated microwave signal can cover multi bands from S band to Ka band. A tunable multiband microwave signal spanning from 5 GHz to 40 GHz can be generated by the beating between the optical carrier and injection locked modulation sidebands in a photodetector without an optical filter. The wavelength of the slave laser can be continuously and near-linearly adjusted by proper changing its bias current. By tuning the bias current of the slave laser, the wavelength of that is matched to one of the modulation sidebands of the OFCG. The performance of the arrangement in terms of the tunability and stability of the generated microwave signal is also studied.

  10. Real-time digital signal processing for live electro-optic imaging.

    PubMed

    Sasagawa, Kiyotaka; Kanno, Atsushi; Tsuchiya, Masahiro

    2009-08-31

    We present an imaging system that enables real-time magnitude and phase detection of modulated signals and its application to a Live Electro-optic Imaging (LEI) system, which realizes instantaneous visualization of RF electric fields. The real-time acquisition of magnitude and phase images of a modulated optical signal at 5 kHz is demonstrated by imaging with a Si-based high-speed CMOS image sensor and real-time signal processing with a digital signal processor. In the LEI system, RF electric fields are probed with light via an electro-optic crystal plate and downconverted to an intermediate frequency by parallel optical heterodyning, which can be detected with the image sensor. The artifacts caused by the optics and the image sensor characteristics are corrected by image processing. As examples, we demonstrate real-time visualization of electric fields from RF circuits.

  11. High-power optical millimeter-wave signal generation with tunable frequency multiplication factor

    NASA Astrophysics Data System (ADS)

    Han, Yi-shi; Zheng, Zhenyu; Luo, Zhixiao; Min, Zhixuan; Xu, Ou; Liu, Jie

    2015-01-01

    This work demonstrates a simple and novel scheme for millimeter-wave (MMW) signal generation using optical multi-sidebands (OMSB) modulation. In the proposed methods, several pairs of optical sidebands can be generated by employing parallel phase modulators driven by a low frequency radio frequency (RF) signal. The optical sidebands will beat at a photodetector (PD) to generate high frequency MMW signal with tunable frequency multiplication factor, such as frequency octupling, 12-tupling, 16-tupling and 18-tupling. Since no optical filters or DC bias are used, the MMW signal has the evident character of high-power output. A generalized analytic expression and simulation verification for generating the frequency multi-tupling MMW signal are developed. The influences caused by non-ideal factors are discussed in detail, and undesired power ratios versus non-ideal factors are plotted and analyzed.

  12. A Multi-Functional Planar Lightwave Circuit for Optical Signal Processing Applications

    NASA Astrophysics Data System (ADS)

    Samadi, Payman

    Ultrafast optical signal processing is now a necessary tool in several domains of science and technology such as high-speed telecommunication, biomedicine, microscopy and radar systems. Optical arbitrary waveform generation is an optical signal processing function which has applications in optical telecommunication networks, sampling, and photonically-assisted RF waveform generation. Furthermore, performing optical signal processing in photonic integrated circuits is crucial for system integration and overcoming the speed limitations in electrical to optical conversion. In this thesis, we introduce a silica-based planar lightwave circuit which performs several optical signal processing functions. We start by reviewing the material system used to fabricate the device. We justify the choice of the material for our application and explain the fabrication process and the experiments to characterize the device. Then we introduce the fundamental theory of our device which is based on pulse repetition rate multiplication (PRRM) and shaping. We review the theory of direct time-domain approach to perform the PRRM and shaping. Experiments to measure the impulse response of the device, perform PRRM and polarization dependence characterization is shown as well. Three main applications of our device is presented next. First we use the PLC device with non-linear optics to generate multiple pulse trains at different wavelengths and different repetition rates. Second, we use the fundamental of the previous application to perform demultiplexing of optical time division multiplexed signals. Our approach is flexible in a sense that it can demultiplex any tributary channel of lower rate data, also it works for both amplitude and phase modulated data. Finally, using the second generation of our PLC device, we photonically generate radio frequency waveforms. We are able to generate various pulse shapes which are generally hard to generate using electronics at frequencies up to 80 GHz

  13. Intrinsic differences in the initiation of B cell receptor signaling favor responses of human IgG(+) memory B cells over IgM(+) naive B cells.

    PubMed

    Davey, Angel M; Pierce, Susan K

    2012-04-01

    The acquisition of long-lived memory B cells (MBCs) is critical for the defense against many infectious diseases. Despite their importance, little is known about how Ags trigger human MBCs, even though our understanding of the molecular basis of Ag activation of B cells in model systems has advanced considerably. In this study, we use quantitative, high-resolution, live-cell imaging at the single-cell and single-molecule levels to describe the earliest Ag-driven events in human isotype-switched, IgG-expressing MBCs and compare them with those in IgM-expressing naive B cells. We show that human MBCs are more robust than naive B cells at each step in the initiation of BCR signaling, including interrogation of Ag-containing membranes, formation of submicroscopic BCR oligomers, and recruitment and activation of signaling-associated kinases. Despite their robust response to Ag, MBCs remain highly sensitive to FcγRIIB-mediated inhibition. We also demonstrate that in the absence of Ag, a portion of MBC receptors spontaneously oligomerized, and phosphorylated kinases accumulated at the membrane and speculate that heightened constitutive signaling may play a role in maintaining MBC longevity. Using high-resolution imaging, we have provided a description of the earliest events in the Ag activation of MBCs and evidence for acquired cell-intrinsic differences in the initiation of BCR signaling in human naive and MBCs.

  14. Quality Monitoring of Infrared Optics Using Ultrasound Signals

    NASA Astrophysics Data System (ADS)

    Neumeier, Benedikt; Schmitt-Landsiedel, Doris

    During laser processing with beam powers in the multi-kilowatt range a fraction of the emission is absorbed in optical elements, leading to thermal loading on the optics. This temperature rise of the optics results in thermal lensing, impairing the power density at the processing location. In a number of niche applications, the CO2 laser still is preferred over solid-state lasers due to its unrivaled features in processing materials like fused silica, glasses, wood, ceramics or plastics. Applying existing techniques to monitor the temperature of CO2 laser optics using pyrometers prove to be unreliable due to the transmissivity in the infrared of the optical materials used. The object of this study is experimental identification of temperature increase of ZnSe optics during use for processing in a laser cutting machine, and development of a method utilizing ultrasound to measure the temperature of the infrared optics. The method enables the testing of the absorption properties of infrared optics as well as solid-state laser optics.

  15. Influence of intrinsic signals and environmental cues on the endocrine control of feeding in fish: potential application in aquaculture.

    PubMed

    Volkoff, Hélène; Hoskins, Leah J; Tuziak, Sarah M

    2010-07-01

    Optimization of food consumption and ultimately growth are major concerns for aquaculture. In fish, food intake is regulated by several hormones produced by both brain and peripheral tissues. Changes in feeding behavior and appetite usually occur through the modulation of the gene expression and/or action of these appetite-regulating hormones and can be due not only to variations in intrinsic factors such as nutritional/metabolic or reproductive status, but also to changes in environmental factors, such as temperature and photoperiod. In addition, the gene expression and/or plasma levels of appetite-regulating hormones might also display daily as well as circannual (seasonal) rhythms. Despite recent advances, our current understanding of the regulation of feeding in fish is still limited. We give here a brief overview of our current knowledge of the endocrine regulation of feeding in fish and describe how a better understanding of appetite-related hormones in fish might lead to the development of sustainable aquaculture.

  16. Photoelectricity signal processing circuit of interferometric fiber optic pressure sensor

    NASA Astrophysics Data System (ADS)

    Guo, Zhenwu; Li, Wei-xiang; Meng, Qing-bin; Pan, Yong; Liu, Guang-wei; Ge, Fu-wei; Zhang, Rong-xin

    2009-07-01

    We have designed an intensity-demodulated sensing system based on Fabry-Perot interferometric sensor for pressure measurement. The structure of the sensing probe has been presented. The sensing system is interrogated by broadband source. For compensating drift of the source power and fluctuation in fiber attenuation, the light beam is separated into two channels by a fiber Bragg Grating, the transmitted light used as reference signal and the reflected light used as sensing signal. In order to improve the signal-to-noise ratio(SNR) of the detection system, the input light is modulated by pulse signal, and the low noise preamplifier is given. The more important factor to improve the SNR is that a synchronization integrator is employed to construct a narrow band filter to restrain noises and disturbances. It has better performance with a narrow band noise filter rather than the general RC active bandpass filter. The sensing signal and the reference signal are transformed into DC voltage signal from AC voltage signal after they passed the synchronization integrator circuit. Subsequently the division operation of the sensing signal and the reference signal is implemented. At last a linear output model is established. The system has advantages of fast response, strong ability and low cost. The dynamic range of the sensor is from 0 to 400KPa, and the resolution reaches to 200Pa.

  17. Removal Of Surface Deposits And Intrinsic Stains Of Teeth With Fiber Optics Of Nd-YAG Laser

    NASA Astrophysics Data System (ADS)

    Marioka, Toshio; Maseda, Yusaku; Oho, Takahiko

    1987-03-01

    An impact of the Q-switched Nd-YAG laser caused bleaching of stains and removal of deposits and pit & fissure contents of teeth. No chalky spots or craters were found microscopically on the enamel surface after irradiation. These results strongly suggested the clinical applicability of Q-switched Nd-YAG laser in removing dental deposits, intrinsic pigmentation of enamel, and pit and fissure contents of molar.

  18. PAPR reduction in optical OFDM systems using asymmetrically clipping and signal scrambling technique

    NASA Astrophysics Data System (ADS)

    Chen, Lin; Fang, Yong; Huang, Qinghua; Sun, Yanzan

    2015-08-01

    Optical orthogonal frequency division multiplexing (OOFDM) is a promising technology in the next generation of high-speed and long-haul optical transmission, due to its high spectral efficiency, high speed of data transmission and strong ability of anti-dispersion. But optical OFDM system has a very high peak-to-average power ratio (PAPR). High PAPR will bring instantaneous high optical power to the optical OFDM system. Asymmetrically clipping and signal scrambling based on fast Hartley transform for PAPR reduction is proposed in optical OFDM system. Firstly, IFFT/FFT module in each sub-block of traditional signal scrambling technique is replaced with inverse fast Hartley transform (IFHT) and fast Hartley transform (FHT) module, which yield to the real signal in OOFDM system. Then, asymmetrically clipping technique is applied to turn it into a positive and real signal. Finally, the signal with the minimum PAPR is selected for transmission in the fiber channel. The PAPR of the optical OFDM signal can be reduced effectively. And without the Hermitian symmetry, the space and computational complexity are reduced accordingly.

  19. Coherent Optical Receiver for PPM Signals under Atmospheric Turbulence

    NASA Technical Reports Server (NTRS)

    Munoz Fernandez, Michela; Vilnrotter, Victor A.

    2005-01-01

    Adaptive combining of experimentally obtained heterodyned pulse position modulated (PPM) signals with pulse-to-pulse coherence in the presence of simulated spatial distortions resembling atmospheric turbulence is demonstrated. The adaptively combined PPM signals are phased up via an LMS algorithm suitably optimized to operate with PPM in the presence of additive shot-noise. A convergence analysis of the algorithm is presented, and results with both, computer simulated and experimentally obtained PPM signals are analyzed.

  20. Photonic integrated circuit for all-optical millimeter-wave signal generation

    SciTech Connect

    Vawter, G.A.; Mar, A.; Zolper, J.; Hietala, V.

    1997-03-01

    Generation of millimeter-wave electronic signals and power is required for high-frequency communication links, RADAR, remote sensing and other applications. However, in the 30 to 300 GHz mm-wave regime, signal sources are bulky and inefficient. All-optical generation of mm-wave signals promises to improve efficiency to as much as 30 to 50 percent with output power as high as 100 mW. All of this may be achieved while taking advantage of the benefits of monolithic integration to reduce the overall size to that of a single semiconductor chip only a fraction of a square centimeter in size. This report summarizes the development of the first monolithically integrated all-optical mm-wave signal generator ever built. The design integrates a mode-locked semiconductor ring diode laser with an optical amplifier and high-speed photodetector into a single optical integrated circuit. Frequency generation is demonstrated at 30, 60 and 90 Ghz.

  1. Optical radio-photonic channel for transmission of a coherent narrowband analog signal

    NASA Astrophysics Data System (ADS)

    Zhuk, D. I.; Denisyuk, I. Yu.; Fokina, M. I.

    2015-10-01

    The channel of an optical transmission line of coherent narrowband analog signal consisting of a continuous-wave laser, an electro-optic modulator, and a vector phase rotator based on electrically controlled fiber-optical 1 × 2 splitter and fixed delay lines is analyzed. The scheme is constructed from commercially available components used in digital optical communication systems. The applicability of components for analog and small-signal circuits is determined. Variation of radio signal phase in the range from 0° to 170° for radio signal frequencies between 1 and 2 GHz is demonstrated experimentally. It is shown that phase variation is a linear function of frequency in this range.

  2. Microwave vector signal transmission over an optical fiber based on IQ modulation and coherent detection.

    PubMed

    Chen, Yang; Shao, Tong; Wen, Aijun; Yao, Jianping

    2014-03-15

    A novel approach to transmitting two vector signals using a single optical carrier based on IQ modulation and coherent detection is proposed and demonstrated. In the proposed system, two quadrature phase-shift keying (QPSK) signals are IQ modulated on an optical carrier with one polarization state using a dual-parallel Mach-Zehnder modulator (DP-MZM). The optical carrier with an orthogonal polarization state is not modulated but transmitted with the modulated optical wave. At the receiver, the two orthogonally polarized light waves are separated and sent to a coherent detector, where the two QPSK signals are separated and demodulated. An experiment is performed. The transmission of two QPSK signals at 2 GHz with a data rate of 1 Gbps is implemented over a 25 km single-mode fiber. The performance of the transmission in terms of error vector magnitude is evaluated.

  3. All-optical signal amplifier and distributor using cavity-atom coupling systems

    NASA Astrophysics Data System (ADS)

    Duan, Yafan; Lin, Gongwei; Niu, Yueping; Gong, Shangqing

    2016-05-01

    We report an all-optical signal amplifier and a signal distributor using cavity-atom coupling systems. In this system we couple atoms with an optical cavity and realize the great enhancement of a control laser by the cavity with the help of two high coupling lasers. By this effect, we can use one weak control field to control another strong target field and the intensity changes are linear with our experimental conditions. This can be used as an all-optical signal amplifier, also known as a ‘transphasor’. In our experiment, the gain of the weak field to strong field can be as high as 60. Furthermore, we can realize the distribution of optical signals, if we coordinate multiple cavity-atom coupling systems.

  4. From the intrinsic properties to the functional role of a neuron phenotype: an example from electric fish during signal trade-off.

    PubMed

    Nogueira, Javier; Caputi, Angel A

    2013-07-01

    This review deals with the question: what is the relationship between the properties of a neuron and the role that the neuron plays within a given neural circuit? Answering this kind of question requires collecting evidence from multiple neuron phenotypes and comparing the role of each type in circuits that perform well-defined computational tasks. The focus here is on the spherical neurons in the electrosensory lobe of the electric fish Gymnotus omarorum. They belong to the one-spike-onset phenotype expressed at the early stages of signal processing in various sensory modalities and diverse taxa. First, we refer to the one-spike neuron intrinsic properties, their foundation on a low-threshold K(+) conductance, and the potential roles of this phenotype in different circuits within a comparative framework. Second, we present a brief description of the active electric sense of weakly electric fish and the particularities of spherical one-spike-onset neurons in the electrosensory lobe of G. omarorum. Third, we introduce one of the specific tasks in which these neurons are involved: the trade-off between self- and allo-generated signals. Fourth, we discuss recent evidence indicating a still-undescribed role for the one-spike phenotype. This role deals with the blockage of the pathway after being activated by the self-generated electric organ discharge and how this blockage favors self-generated electrosensory information in the context of allo-generated interference. Based on comparative analysis we conclude that one-spike-onset neurons may play several functional roles in animal sensory behavior. There are specific adaptations of the neuron's 'response function' to the circuit and task. Conversely, the way in which a task is accomplished depends on the intrinsic properties of the neurons involved. In short, the role of a neuron within a circuit depends on the neuron and its functional context. PMID:23761463

  5. From the intrinsic properties to the functional role of a neuron phenotype: an example from electric fish during signal trade-off.

    PubMed

    Nogueira, Javier; Caputi, Angel A

    2013-07-01

    This review deals with the question: what is the relationship between the properties of a neuron and the role that the neuron plays within a given neural circuit? Answering this kind of question requires collecting evidence from multiple neuron phenotypes and comparing the role of each type in circuits that perform well-defined computational tasks. The focus here is on the spherical neurons in the electrosensory lobe of the electric fish Gymnotus omarorum. They belong to the one-spike-onset phenotype expressed at the early stages of signal processing in various sensory modalities and diverse taxa. First, we refer to the one-spike neuron intrinsic properties, their foundation on a low-threshold K(+) conductance, and the potential roles of this phenotype in different circuits within a comparative framework. Second, we present a brief description of the active electric sense of weakly electric fish and the particularities of spherical one-spike-onset neurons in the electrosensory lobe of G. omarorum. Third, we introduce one of the specific tasks in which these neurons are involved: the trade-off between self- and allo-generated signals. Fourth, we discuss recent evidence indicating a still-undescribed role for the one-spike phenotype. This role deals with the blockage of the pathway after being activated by the self-generated electric organ discharge and how this blockage favors self-generated electrosensory information in the context of allo-generated interference. Based on comparative analysis we conclude that one-spike-onset neurons may play several functional roles in animal sensory behavior. There are specific adaptations of the neuron's 'response function' to the circuit and task. Conversely, the way in which a task is accomplished depends on the intrinsic properties of the neurons involved. In short, the role of a neuron within a circuit depends on the neuron and its functional context.

  6. System and method for ultrafast optical signal detecting via a synchronously coupled anamorphic light pulse encoded laterally

    DOEpatents

    Heebner, John E.

    2010-08-03

    In one general embodiment, a method for ultrafast optical signal detecting is provided. In operation, a first optical input signal is propagated through a first wave guiding layer of a waveguide. Additionally, a second optical input signal is propagated through a second wave guiding layer of the waveguide. Furthermore, an optical control signal is applied to a top of the waveguide, the optical control signal being oriented diagonally relative to the top of the waveguide such that the application is used to influence at least a portion of the first optical input signal propagating through the first wave guiding layer of the waveguide. In addition, the first and the second optical input signals output from the waveguide are combined. Further, the combined optical signals output from the waveguide are detected. In another general embodiment, a system for ultrafast optical signal recording is provided comprising a waveguide including a plurality of wave guiding layers, an optical control source positioned to propagate an optical control signal towards the waveguide in a diagonal orientation relative to a top of the waveguide, at least one optical input source positioned to input an optical input signal into at least a first and a second wave guiding layer of the waveguide, and a detector for detecting at least one interference pattern output from the waveguide, where at least one of the interference patterns results from a combination of the optical input signals input into the first and the second wave guiding layer. Furthermore, propagation of the optical control signal is used to influence at least a portion of the optical input signal propagating through the first wave guiding layer of the waveguide.

  7. Experimental study of the use of multiband acousto-optic filters for spectral encoding / decoding the optical signals

    NASA Astrophysics Data System (ADS)

    Proklov, V. V.; Byshevski-Konopko, O. A.; Filatov, A. L.; Lugovskoi, A. V.; Pisarevsky, Yu V.

    2016-08-01

    A prototype of the acousto-optic (AO) decoder of optical signals is created on the base of the multiband AO filter. The joint work of the decoder with the developed previously AO coder has been verified experimentally. The main qualitative and quantitate characteristics of the spectral coding and decoding by Walsh sequences of the industrial LED radiation in the near infrared range are investigated. It is shown, that in the proposed data transmission system realization Signal-to-Interference Ratio (SIR) is not less than 13 dB.

  8. A topographic instructive signal guides the adjustment of the auditory space map in the optic tectum.

    PubMed

    Hyde, P S; Knudsen, E I

    2001-11-01

    Maps of auditory space in the midbrain of the barn owl (Tyto alba) are calibrated by visual experience. When owls are raised wearing prismatic spectacles that displace the visual field in azimuth, the auditory receptive fields of neurons in the optic tectum shift to compensate for the optical displacement of the visual field. This shift results primarily from a shift in the tuning of tectal neurons for interaural time difference. The visually based instructive signal that guides this plasticity could be based on a topographic, point-by-point comparison between auditory and visual space maps or on a foveation-dependent visual assessment of the accuracy of auditory orienting responses. To distinguish between these two possibilities, we subjected owls to optical conditions that differed in the center of gaze and the visual periphery. A topographic signal would cause the portions of the space map representing the central and peripheral regions of visual space to adjust differently, according to the optical conditions that exist in each region. In contrast, a foveation-based signal would cause both portions of the map to adjust similarly, according to the optical conditions that exist at the center of gaze. In six of seven experiments, adaptive changes were as predicted by a topographic instructive signal. Although the results do not rule out the possible contribution of a foveation-based signal, they demonstrate that a topographic instructive signal is, indeed, involved in the calibration of the auditory space map in the barn owl optic tectum. PMID:11606646

  9. Scanning near-field optical microscopy signal processing and resolution.

    PubMed

    Grosges, Thomas; Barchiesi, Dominique

    2007-04-20

    To increase the signal-to-noise ratio and to remove the spatially slow varying signals, a lock-in amplifier is often used in scanning probe microscopy. The signal reconstructed from the lock-in data contains the contributions of the evanescent and homogeneous waves that are mixed in the near-field zone (i.e., at a very short distance). The resolution is determined and a method is given to suppress the useless background information. Experimental images of nanoparticles are processed.

  10. Optical fiber sensors and signal processing for intelligent structure monitoring

    NASA Technical Reports Server (NTRS)

    Rogowski, Robert; Claus, R. O.; Lindner, D. K.; Thomas, Daniel; Cox, Dave

    1988-01-01

    The analytic and experimental performance of optical fiber sensors for the control of vibration of large aerospace and other structures are investigated. In particular, model domain optical fiber sensor systems, are being studied due to their apparent potential as distributed, low mass sensors of vibration over appropriate ranges of both low frequency and low amplitude displacements. Progress during the past three months is outlined. Progress since September is divided into work in the areas of experimental hardware development, analytical analysis, control design and sensor development. During the next six months, tests of a prototype closed-loop control system for a beam are planned which will demonstrate the solution of several optical fiber instrumentation device problems, the performance of the control system theory which incorporates the model of the modal domain sensor, and the potential for distributed control which this sensor approach offers.

  11. On-Board Fiber-Optic Network Architectures for Radar and Avionics Signal Distribution

    NASA Technical Reports Server (NTRS)

    Alam, Mohammad F.; Atiquzzaman, Mohammed; Duncan, Bradley B.; Nguyen, Hung; Kunath, Richard

    2000-01-01

    Continued progress in both civil and military avionics applications is overstressing the capabilities of existing radio-frequency (RF) communication networks based on coaxial cables on board modem aircrafts. Future avionics systems will require high-bandwidth on- board communication links that are lightweight, immune to electromagnetic interference, and highly reliable. Fiber optic communication technology can meet all these challenges in a cost-effective manner. Recently, digital fiber-optic communication systems, where a fiber-optic network acts like a local area network (LAN) for digital data communications, have become a topic of extensive research and development. Although a fiber-optic system can be designed to transport radio-frequency (RF) signals, the digital fiber-optic systems under development today are not capable of transporting microwave and millimeter-wave RF signals used in radar and avionics systems on board an aircraft. Recent advances in fiber optic technology, especially wavelength division multiplexing (WDM), has opened a number of possibilities for designing on-board fiber optic networks, including all-optical networks for radar and avionics RF signal distribution. In this paper, we investigate a number of different novel approaches for fiber-optic transmission of on-board VHF and UHF RF signals using commercial off-the-shelf (COTS) components. The relative merits and demerits of each architecture are discussed, and the suitability of each architecture for particular applications is pointed out. All-optical approaches show better performance than other traditional approaches in terms of signal-to-noise ratio, power consumption, and weight requirements.

  12. Optical fiber sensors and signal processing for intelligent structure monitoring

    NASA Technical Reports Server (NTRS)

    Thomas, Daniel; Cox, Dave; Lindner, D. K.; Claus, R. O.

    1989-01-01

    Few mode optical fibers have been shown to produce predictable interference patterns when placed under strain. The use is described of a modal domain sensor in a vibration control experiment. An optical fiber is bonded along the length of a flexible beam. Output from the modal domain sensor is used to suppress vibrations induced in the beam. A distributed effect model for the modal domain sensor is developed. This model is combined with the beam and actuator dynamics to produce a system suitable for control design. Computer simulations predict open and closed loop dynamic responses. An experimental apparatus is described and experimental results are presented.

  13. Electro-optical microwave signal processor for high-frequency wideband frequency channelization

    NASA Astrophysics Data System (ADS)

    Dawber, William N.; Webster, Ken

    1998-08-01

    An electro-optic microwave signal processor for activity monitoring in an electronic warfare receiver, offering wideband operation, parallel output in real time and 100 percent probability of intercept is presented, along with results from a prototype system. Requirements on electronic warfare receiver system are demanding, because they have to defect and identify potential threats across a large frequency bandwidth and in the high pulse density expected of the battlefield environment. A technique of processing signals across a wide bandwidth is to use a channelizer in the receiver front-end, in order to produce a number of narrow band outputs that can be individually processed. In the presented signal processor, received microwave signals ar unconverted onto an optical carrier using an electro- optic modulator and then spatially separated into a series of spots. The position and intensity of the spots is determined by the received signal(s) frequency and strength. Finally a photodiode array can be used for fast parallel data readout. Thus the signal processor output is fully channelized according to frequency. A prototype signal processor has been constructed, which can process microwave frequencies from 500MHz to 8GHz. A standard telecommunications electro-optic intensity modulator with a 3dB bandwidth of approximately 2.5GHz provides frequency upconversion. Readout is achieved using either a near IR camera or a 16 element linear photodiode array.

  14. Secure chaotic transmission of electrocardiography signals with acousto-optic modulation under profiled beam propagation.

    PubMed

    Almehmadi, Fares S; Chatterjee, Monish R

    2015-01-10

    Electrocardiography (ECG) signals are used for both medical purposes and identifying individuals. It is often necessary to encrypt this highly sensitive information before it is transmitted over any channel. A closed-loop acousto-optic hybrid device acting as a chaotic modulator is applied to ECG signals to achieve this encryption. Recently improved modeling of this approach using profiled optical beams has shown it to be very sensitive to key parameters that characterize the encryption and decryption process, exhibiting its potential for secure transmission of analog and digital signals. Here the encryption and decryption is demonstrated for ECG signals, both analog and digital versions, illustrating strong encryption without significant distortion. Performance analysis pertinent to both analog and digital transmission of the ECG waveform is also carried out using output signal-to-noise, signal-to-distortion, and bit-error-rate measures relative to the key parameters and presence of channel noise in the system.

  15. Performance evaluation of peak-clipped optical BPSK-SSB modulated signal.

    PubMed

    Sampath, K I Amila; Takano, Katsumi

    2015-07-27

    This study examines the performance of peak-clipped optical BPSK-SSB signal. The effectiveness of peak clipping for PAPR reduction and the degradation caused by peak clipping are numerically analyzed. PAPR of optical BPSK-SSB signal becomes high because of the peaky Hilbert-transformed signal component. PAPR improvement of 43.7% is attained by clipping the peaks of the Hilbert-transformed signal. Assessment of spectral degradation reveals that both waveform clipping and modulator nonlinearity contribute to sideband suppression degradation. Analyses of the 100-km transmitted signal results show that PAPR reduction by peak clipping alleviates the nonlinear phase shift caused by self-phase modulation (SPM), which produces a less degraded signal at the detector. Peak clipping can improve the SPM threshold of the studied system by 2.63 dB. PMID:26367640

  16. Low-frequency analog signal distribution on digital photonic networks by optical delta-sigma modulation

    NASA Astrophysics Data System (ADS)

    Kanno, Atsushi; Kawanishi, Tetsuya

    2013-12-01

    We propose a delta-sigma modulation scheme for low- and medium-frequency signal transmission in a digital photonic network system. A 10-Gb/s-class optical transceiver with a delta-sigma modulator utilized as a high-speed analog-to-digital converter (ADC) provides a binary optical signal. On the signal reception side, a low-cost and slow-speed photonic receiver directly converts the binary signal into an analog signal at frequencies from several hundreds of kilohertz several tens of megahertz. Further, by using a clock and data recovery circuit at the receiver to reduce jitters, the single-sideband phase noise of the generated signals can be significantly reduced.

  17. Photoacoustic correlation signal-to-noise ratio enhancement by coherent averaging and optical waveform optimization.

    PubMed

    Telenkov, Sergey A; Alwi, Rudolf; Mandelis, Andreas

    2013-10-01

    Photoacoustic (PA) imaging of biological tissues using laser diodes instead of conventional Q-switched pulsed systems provides an attractive alternative for biomedical applications. However, the relatively low energy of laser diodes operating in the pulsed regime, results in generation of very weak acoustic waves, and low signal-to-noise ratio (SNR) of the detected signals. This problem can be addressed if optical excitation is modulated using custom waveforms and correlation processing is employed to increase SNR through signal compression. This work investigates the effect of the parameters of the modulation waveform on the resulting correlation signal and offers a practical means for optimizing PA signal detection. The advantage of coherent signal averaging is demonstrated using theoretical analysis and a numerical model of PA generation. It was shown that an additional 5-10 dB of SNR can be gained through waveform engineering by adjusting the parameters and profile of optical modulation waveforms.

  18. Effect of second order signal-noise interactions in nonlinearity compensated optical transmission systems.

    PubMed

    Al-Khateeb, Mohammad A Z; McCarthy, Mary; Sánchez, Christian; Ellis, Andrew

    2016-04-15

    In this Letter, we theoretically and numerically analyze the performance of coherent optical transmission systems that deploy inline or transceiver based nonlinearity compensation techniques. For systems where signal-signal nonlinear interactions are fully compensated, we find that beyond the performance peak the signal-to-noise ratio degradation has a slope of 3 dBSNR/dBPower suggesting a quartic rather than quadratic dependence on signal power. This is directly related to the fact that signals in a given span will interact not only with linear amplified spontaneous emission noise, but also with the nonlinear four-wave mixing products generated from signal-noise interaction in previous (hitherto) uncompensated spans. The performance of optical systems employing different nonlinearity compensation schemes were numerically simulated and compared against analytical predictions, showing a good agreement within a 0.4 dB margin of error. PMID:27082361

  19. Secure chaotic transmission of electrocardiography signals with acousto-optic modulation under profiled beam propagation.

    PubMed

    Almehmadi, Fares S; Chatterjee, Monish R

    2015-01-10

    Electrocardiography (ECG) signals are used for both medical purposes and identifying individuals. It is often necessary to encrypt this highly sensitive information before it is transmitted over any channel. A closed-loop acousto-optic hybrid device acting as a chaotic modulator is applied to ECG signals to achieve this encryption. Recently improved modeling of this approach using profiled optical beams has shown it to be very sensitive to key parameters that characterize the encryption and decryption process, exhibiting its potential for secure transmission of analog and digital signals. Here the encryption and decryption is demonstrated for ECG signals, both analog and digital versions, illustrating strong encryption without significant distortion. Performance analysis pertinent to both analog and digital transmission of the ECG waveform is also carried out using output signal-to-noise, signal-to-distortion, and bit-error-rate measures relative to the key parameters and presence of channel noise in the system. PMID:25967617

  20. Cellular signaling protective against noise-induced hearing loss – A role for novel intrinsic cochlear signaling involving corticotropin-releasing factor?

    PubMed

    Vetter, Douglas E

    2015-09-01

    Hearing loss afflicts approximately 15% of the world's population, and crosses all socioeconomic boundaries. While great strides have been made in understanding the genetic components of syndromic and non-syndromic hearing loss, understanding of the mechanisms underlying noise-induced hearing loss (NIHL) have come much more slowly. NIHL is not simply a mechanism by which older individuals loose their hearing. Significantly, the incidence of NIHL is increasing, and is now involving ever younger populations. This may predict future increased occurrences of hearing loss. Current research has shown that even short-term exposures to loud sounds generating what was previously considered temporary hearing loss, actually produces an almost immediate and permanent loss of specific populations of auditory nerve fibers. Additionally, recurrent exposures to intense sound may hasten age-related hearing loss. While NIHL is a significant medical concern, to date, few compounds have delivered significant protection, arguing that new targets need to be identified. In this commentary, we will explore cellular signaling processes taking place in the cochlea believed to be involved in protection against hearing loss, and highlight new data suggestive of novel signaling not previously recognized as occurring in the cochlea, that is perhaps protective of hearing. This includes a recently described local hypothalamic-pituitary-adrenal axis (HPA)-like signaling system fully contained in the cochlea. This system may represent a local cellular stress-response system based on stress hormone release similar to the systemic HPA axis. Its discovery may hold hope for new drug therapies that can be delivered directly to the cochlea, circumventing systemic side effects.

  1. Theoretical and Experimental Study of Optical Coherence Tomography (OCT) Signals Using an Analytical Transport Model

    SciTech Connect

    Vazquez Villa, A.; Delgado Atencio, J. A.; Vazquez y Montiel, S.; Cunill Rodriguez, M.; Martinez Rodriguez, A. E.; Ramos, J. Castro; Villanueva, A.

    2010-12-07

    Optical coherence tomography (OCT) is a non-invasive low coherent interferometric technique that provides cross-sectional images of turbid media. OCT is based on the classical Michelson interferometer where the mirror of the reference arm is oscillating and the signal arm contains a biological sample. In this work, we analyzed theoretically the heterodyne optical signal adopting the so called extended Huygens-Fresnel principle (EHFP). We use simulated OCT images with known optical properties to test an algorithm developed by ourselves to recover the scattering coefficient and we recovered the scattering coefficient with a relative error less than 5% for noisy signals. In addition, we applied this algorithm to OCT images from phantoms of known optical properties; in this case curves were indistinguishable. A revision of the validity of the analytical model applied to our system should be done.

  2. RZ-DQPSK Signal Amplitude Regeneration Using a Semiconductor Optical Amplifier

    NASA Astrophysics Data System (ADS)

    Wu, Wen-Han; Huang, Xi; Yu, Yu; Zhang, Xin-Liang

    2012-04-01

    An all-optical return-to-zero differential quadrature phase-shift keying (RZ-DQPSK) signal regeneration scheme is experimentally demonstrated. Thanks to the gain saturation effect, common quantum-well semiconductor optical amplifiers have the ability to regenerate the amplitude distorted RZ-DQPSK signal, so amplitude noise can be reduced while phase information will not be distorted. A significant eye-opening improvement and a negative power penalty of about 1 dB can be achieved for an 80 Gb/s RZ-DQPSK signal.

  3. Integrated optical modulator for signal up-conversion over radio-on-fiber link.

    PubMed

    Kim, Woo-Kyung; Kwon, Soon-Woo; Jeong, Woo-Jin; Son, Geun-Sik; Lee, Kwang-Hyun; Choi, Woo-Young; Yang, Woo-Seok; Lee, Hyung-Man; Lee, Han-Young

    2009-02-16

    An integrated optical modulator, which consists of a dual-sideband suppressed carrier (DSB-SC) modulator cascaded with a single-sideband (SSB) modulator, is proposed for signal up-conversion over Radio-on-Fiber. Utilizing a single-drive domain inverted structure in both modulators, balanced modulations were obtained without complicated radio frequency (RF) driving circuits and delicate RF phase adjustments. Intermediate frequency (IF) band signal was up-conversed to 60GHz band by using the fabricated device and was transmitted over optical fiber. Experiment results show that the proposed device enables millimeter wave generation and signal transmission without any power penalty caused by chromatic dispersion.

  4. Transmission of independent signals through a multimode fiber using digital optical phase conjugation.

    PubMed

    Czarske, Jürgen W; Haufe, Daniel; Koukourakis, Nektarios; Büttner, Lars

    2016-06-27

    Multimode fibers are attractive for a variety of applications such as communication engineering and biophotonics. However, a major hurdle for the optical transmission through multimode fibers is the inherent mode mixing. Although an image transmission was successfully accomplished using wavefront shaping, the image information was not transmitted individually for each of the independent pixels. We demonstrate a transmission of independent signals using individually shaped wavefronts employing a single segmented spatial light modulator for optical phase conjugation regarding each light signal. Our findings pave the way towards transferring independent signals through strongly scattering media. PMID:27410664

  5. High Speed All Optical Nyquist Signal Generation and Full-band Coherent Detection

    PubMed Central

    Zhang, Junwen; Yu, Jianjun; Fang, Yuan; Chi, Nan

    2014-01-01

    Spectrum efficient data transmission is of key interest for high capacity optical communication systems considering the limited available bandwidth. Transmission of the high speed signal with higher-order modulation formats within the Nyquist bandwidth using coherent detection brings attractive performance advantages. However, high speed Nyquist signal generation with high order modulation formats is challenging. Electrical Nyquist pulse generation is restricted by the limited sampling rate and processor capacities of digital-to-analog convertor devices, while the optical Nyquist signals can provide a much higher symbol rate using time domain multiplexing method. However, most optical Nyquist signals are based on direct detection with simple modulation formats. Here we report the first experimental demonstration of high speed all optical Nyquist signal generation based on Sinc-shaped pulse generation and time-division multiplexing with high level modulation format and full-band coherent detection. Our experiments demonstrate a highly flexible and compatible all optical high speed Nyquist signal generation and detection scheme for future fiber communication systems. PMID:25142269

  6. High speed all optical Nyquist signal generation and full-band coherent detection.

    PubMed

    Zhang, Junwen; Yu, Jianjun; Fang, Yuan; Chi, Nan

    2014-08-21

    Spectrum efficient data transmission is of key interest for high capacity optical communication systems considering the limited available bandwidth. Transmission of the high speed signal with higher-order modulation formats within the Nyquist bandwidth using coherent detection brings attractive performance advantages. However, high speed Nyquist signal generation with high order modulation formats is challenging. Electrical Nyquist pulse generation is restricted by the limited sampling rate and processor capacities of digital-to-analog convertor devices, while the optical Nyquist signals can provide a much higher symbol rate using time domain multiplexing method. However, most optical Nyquist signals are based on direct detection with simple modulation formats. Here we report the first experimental demonstration of high speed all optical Nyquist signal generation based on Sinc-shaped pulse generation and time-division multiplexing with high level modulation format and full-band coherent detection. Our experiments demonstrate a highly flexible and compatible all optical high speed Nyquist signal generation and detection scheme for future fiber communication systems.

  7. Size-dependent permittivity and intrinsic optical anisotropy of nanometric gold thin films: a density functional theory study.

    PubMed

    Laref, Slimane; Cao, Jiangrong; Asaduzzaman, Abu; Runge, Keith; Deymier, Pierre; Ziolkowski, Richard W; Miyawaki, Mamoru; Muralidharan, Krishna

    2013-05-20

    Physical properties of materials are known to be different from the bulk at the nanometer scale. In this context, the dependence of optical properties of nanometric gold thin films with respect to film thickness is studied using density functional theory (DFT). We find that the in-plane plasma frequency of the gold thin film decreases with decreasing thickness and that the optical permittivity tensor is highly anisotropic as well as thickness dependent. Quantitative knowledge of planar metal film permittivity's thickness dependence can improve the accuracy and reliability of the designs of plasmonic devices and electromagnetic metamaterials. The strong anisotropy observed may become an alternative method of realizing indefinite media.

  8. Research and implementation of the burst-mode optical signal bit-error test

    NASA Astrophysics Data System (ADS)

    Huang, Qiu-yuan; Ma, Chao; Shi, Wei; Chen, Wei

    2009-08-01

    On the basis of the characteristic of TDMA uplink optical signal of PON system, this article puts forward a method of high-speed optical burst bit-error rate testing based on FPGA. The article proposes a new method of generating the burst signal pattern include user-defined pattern and pseudo-random pattern, realizes the slip synchronization, self-synchronization of error detection using data decomposition technique and the traditional irrigation code synchronization technology, completes high-speed burst signal clock synchronization using the rapid synchronization technology of phase-locked loop delay in the external circuit and finishes the bit-error rate test of high-speed burst optical signal.

  9. About the effects of polarising optics on lidar signals and the Δ90 calibration

    NASA Astrophysics Data System (ADS)

    Freudenthaler, Volker

    2016-08-01

    This paper provides a model for assessing the effects of polarising optics on the signals of typical lidar systems, which is based on the description of the individual optical elements of the lidar and of the state of polarisation of the light by means of the Müller-Stokes formalism. General analytical equations are derived for the dependence of the lidar signals on polarisation parameters, for the linear depolarisation ratio, and for the signals of different polarisation calibration setups. The equations can also be used for the calculation of systematic errors caused by nonideal optical elements, their rotational misalignment, and by non-ideal laser polarisation. We present the description of the lidar signals including the polarisation calibration in a closed form, which can be applied for a large variety of lidar systems.

  10. Intrinsic spontaneous emission-induced fluctuations of the output optical beam power and phase in a diode amplifier

    NASA Astrophysics Data System (ADS)

    Bogatov, A. P.; Drakin, A. E.; D'yachkov, N. V.; Gushchik, T. I.

    2016-08-01

    Output optical beam intensity and phase fluctuations are analysed in a classical approach to describing the propagation and amplification of spontaneous emission in the active region of a laser diode with a gain saturated by input monochromatic light. We find their spectral densities and dispersion and the correlation coefficient of the two-dimensional probability distribution function of the fluctuations.

  11. A novel optical millimeter-wave signal generation approach to overcome chromatic dispersion

    NASA Astrophysics Data System (ADS)

    Liang, Dong; Jiang, Wei; Tan, Qinggui; Zhu, Zhongbo; Liu, Feng

    2014-06-01

    In this paper, a novel frequency octupling approach for optical millimeter-wave signal generation to overcome chromatic dispersion is proposed and demonstrated. The frequency octupling mm-wave with the baseband signal carried only by -4th order sideband is generated by properly adjusting a series of parameters, which are the modulation constant, the gain of baseband signal, the direct current bias and the different phase of the modulation arms. As the optical millimeter-wave signal is transmitted along the fiber, there is no time shift caused by chromatic dispersion. Theoretical analyses and simulated results show that when the optical mm-wave carrying 2.5 Gbps baseband signal transmits a distance of over 110 km, the eye diagram still keeps open and clear. The power penalty is about 0.4 dB after the optical signal transmits over 40 km. In additions, given the +4th order sideband carries no data, a full-duplex RoF link based on wavelength reuse is built for the uplink. The bidirectional 2.5 Gbps baseband signal could successfully transmit over 40 km with about 0.8 dB power penalty in the simulation. Both theoretical analyses and simulation results show that the full-duplex RoF link has good performance.

  12. Phase sensitive signal analysis for bi-tapered optical fibers

    NASA Astrophysics Data System (ADS)

    Ben Harush Negari, Amit; Jauregui, Daniel; Sierra Hernandez, Juan M.; Garcia Mina, Diego; King, Branden J.; Idehenre, Ighodalo; Powers, Peter E.; Hansen, Karolyn M.; Haus, Joseph W.

    2016-03-01

    Our study examines the transmission characteristics of bi-tapered optical fibers, i.e. fibers that have a tapered down and up span with a waist length separating them. The applications to aqueous and vapor phase biomolecular sensing demand high sensitivity. A bi-tapered optical fiber platform is suited for label-free biomolecular detection and can be optimized by modification of the length, diameter and surface properties of the tapered region. We have developed a phase sensitive method based on interference of two or more modes of the fiber and we demonstrate that our fiber sensitivity is of order 10-4 refractive index units. Higher sensitivity can be achieved, as needed, by enhancing the fiber design characteristics.

  13. Optical feedback signal for ultrashort laser pulse ablation of tissue

    SciTech Connect

    Kim, B.-M.; Feit, M.D.; Rubenchik, A.M.; Mammini, B.M.; Da Silva, L.B.

    1997-07-01

    An optical feedback system for controlled precise tissue ablation is discussed. Our setup includes an ultrashort pulse laser (USPL), and a diagnostic system using analysis of either tissue fluorescence or plasma emission luminescence. Current research is focused on discriminating hard and soft tissues such as bone and spinal cord during surgery using either technique. Our experimental observations exhibit considerable spectroscopic contrast between hard and soft tissue, and both techniques offer promise for a practical diagnostic system.

  14. Joint digital signal processing for superchannel coherent optical communication systems.

    PubMed

    Liu, Cheng; Pan, Jie; Detwiler, Thomas; Stark, Andrew; Hsueh, Yu-Ting; Chang, Gee-Kung; Ralph, Stephen E

    2013-04-01

    Ultra-high-speed optical communication systems which can support ≥ 1Tb/s per channel transmission will soon be required to meet the increasing capacity demand. However, 1Tb/s over a single carrier requires either or both a high-level modulation format (i.e. 1024QAM) and a high baud rate. Alternatively, grouping a number of tightly spaced "sub-carriers" to form a terabit superchannel increases channel capacity while minimizing the need for high-level modulation formats and high baud rate, which may allow existing formats, baud rate and components to be exploited. In ideal Nyquist-WDM superchannel systems, optical subcarriers with rectangular spectra are tightly packed at a channel spacing equal to the baud rate, thus achieving the Nyquist bandwidth limit. However, in practical Nyquist-WDM systems, precise electrical or optical control of channel spectra is required to avoid strong inter-channel interference (ICI). Here, we propose and demonstrate a new "super receiver" architecture for practical Nyquist-WDM systems, which jointly detects and demodulates multiple channels simultaneously and mitigates the penalties associated with the limitations of generating ideal Nyquist-WDM spectra. Our receiver-side solution relaxes the filter requirements imposed on the transmitter. Two joint DSP algorithms are developed for linear ICI cancellation and joint carrier-phase recovery. Improved system performance is observed with both experimental and simulation data. Performance analysis under different system configurations is conducted to demonstrate the feasibility and robustness of the proposed joint DSP algorithms.

  15. Evaluation the development of focal cerebral ischemia in rats by optical imaging based on the spreading depression signals

    NASA Astrophysics Data System (ADS)

    Chen, Shangbin; Feng, Zhe; Zeng, Shaoqun; Luo, Qingming; Li, Pengcheng

    2007-02-01

    Spreading depression (SD) has been found involved in focal cerebral ischemia which may result in severe or lethal neurological deficits. Electrical recording of SD has been used for acute and long term monitoring of focal cerebral ischemia but with an inherently low resolution. Here, we presented optical intrinsic signal imaging (OISI) to characterize the spontaneous SD waves following permanent middle cerebral artery occlusion (MCAO) in rats with high spatial resolution. During each SD episode, the measured optical reflectance varied regionally: decreased (-12.5+/-2.8%) in the area near the midline, remained flat (3.1+/-2.5%) in the lateral region, and increased (12.1+/-3.6%) in the intermediate cortex. The three types of changes yielded identifications for three biological relevant zones: nonischemic cortex, penumbra and infarct core. Accompanying recurrent SD waves, the suggested penumbral area reduced by about 6.4+/-2.5% of the whole imaged area per SD event, indicating a growth of the infracted area. Staining with 2% 2,3,5-triphenyltetrazolium chloride (TTC) 4 h post-occlusion proved the infarct cortex to be consistent with the lateral region where the final SD wave did not invade (r=0.86+/-0.10). The results suggest that OISI based on SD can effectively used to distinguish nonischemic cortex, penumbra and infarct core in the ischemic hemisphere and monitor the development of ischemia with high spatial resolution.

  16. Determination of critical diameters for intrinsic carrier diffusion-length of GaN nanorods with cryo-scanning near-field optical microscopy

    PubMed Central

    Chen, Y. T.; Karlsson, K. F.; Birch, J.; Holtz, P. O.

    2016-01-01

    Direct measurements of carrier diffusion in GaN nanorods with a designed InGaN/GaN layer-in-a-wire structure by scanning near-field optical microscopy (SNOM) were performed at liquid-helium temperatures of 10 K. Without an applied voltage, intrinsic diffusion lengths of photo-excited carriers were measured as the diameters of the nanorods differ from 50 to 800 nm. The critical diameter of nanorods for carrier diffusion is concluded as 170 nm with a statistical approach. Photoluminescence spectra were acquired for different positions of the SNOM tip on the nanorod, corresponding to the origins of the well-defined luminescence peaks, each being related to recombination-centers. The phenomenon originated from surface oxide by direct comparison of two nanorods with similar diameters in a single map has been observed and investigated. PMID:26876009

  17. All optical millimeter-wave electrical signal generation using an integrated mode-locked semiconductor ring laser and photodiode

    SciTech Connect

    Vawter, G.A.; Mar, A.; Hietala, V.; Zolper, J.; Hohimer, J.

    1997-12-01

    The first monolithic photonic integrated circuit for all-optical generation of millimeter (mm)-wave electrical signals is reported. The design integrates a mode-locked semiconductor ring diode laser, an optical amplifier, and a high-speed photodetector into a single optical integrated circuit. Signal generation is demonstrated at frequencies of 30, 60, and 90 GHz.

  18. Analog CMOS design for optical coherence tomography signal detection and processing.

    PubMed

    Xu, Wei; Mathine, David L; Barton, Jennifer K

    2008-02-01

    A CMOS circuit was designed and fabricated for optical coherence tomography (OCT) signal detection and processing. The circuit includes a photoreceiver, differential gain stage and lock-in amplifier based demodulator. The photoreceiver consists of a CMOS photodetector and low noise differential transimpedance amplifier which converts the optical interference signal into a voltage. The differential gain stage further amplifies the signal. The in-phase and quadrature channels of the lock-in amplifier each include an analog mixer and switched-capacitor low-pass filter with an external mixer reference signal. The interferogram envelope and phase can be extracted with this configuration, enabling Doppler OCT measurements. A sensitivity of -80 dB is achieved with faithful reproduction of the interferometric signal envelope. A sample image of finger tip is presented. PMID:18269983

  19. UWB doublet signal generation and modulation based on DFB laser under optical pulses injection

    NASA Astrophysics Data System (ADS)

    Chen, Dalei; Wang, Rong; Xiang, Peng; Pu, Tao; Fang, Tao; Zhou, Hua; Zhao, Jiyong; Huang, Long; Zhu, Huatao; Wang, Peng

    2016-05-01

    In this paper, a novel scheme to generate ultra-wideband (UWB) doublet signals based on the cross-gain modulation (XGM) effect in the DFB lasers is proposed and experimentally demonstrated, the modulation and transmission of the generated UWB doublet signals are also researched. In the proposed system, a gain-switched laser (GSL) is used as a master laser (ML) and the optical pulses from the ML are optically injected into two paralleled DFB lasers, which are used as slave lasers (SL). Then the outputs from the SLs are detected by a balanced photodiode (BPD) to generate the Bi-phased UWB signals. By properly setting the system parameters, UWB signals with various modulation formats such as on-off keying (OOK), pulse amplitude modulation (PAM) as well as the phase-shift keying (PSK) can be generated. In addition, fiber transmission of the modulated UWB signals is also experimentally investigated.

  20. Wide bandwidth optical signals for high range resolution measurements in water

    NASA Astrophysics Data System (ADS)

    Nash, Justin; Lee, Robert; Mullen, Linda

    2016-05-01

    Measurements with high range resolution are needed to identify underwater threats, especially when two-dimensional contrast information is insufficient to extract object details. The challenge is that optical measurements are limited by scattering phenomena induced by the underwater channel. Back-scatter results in transmitted photons being directed back to the receiver before reaching the target of interest which induces a clutter signal for ranging and a reduction in contrast for imaging. Multiple small-angle scattering (forward-scatter) results in transmitted photons being directed to unintended regions of the target of interest (spatial spreading), while also stretching the temporal profile of a short optical pulse (temporal spreading). Spatial and temporal spreading of the optical signal combine to cause a reduction in range resolution in conventional laser imaging systems. NAVAIR has investigated ways in which wide bandwidth, modulated optical signals can be utilized to improve ranging and imaging performance in turbid water environments. Experimental efforts have been conducted to investigate channel effects on the propagated frequency content, as well as different filtering and processing techniques on the return signals to maximize range resolution. Of particular interest for the modulated pulses are coherent detection and processing techniques employed by the radar community, including methods to reduce sidelobe clutter. This paper will summarize NAVAIR's work and show that wideband optical signals, in combination with the CLEAN algorithm, can indeed provide enhancements to range resolution and 3D imagery in turbid water environments.

  1. Application of a fluorescence intensity ratio technique for the intrinsic determination of pH using an optical fiber sensor

    NASA Astrophysics Data System (ADS)

    Thotath, Bhadra; Nguyen, T. Hien; Zhang, Weiwei; Wren, Stephen P.; Baxter, Gregory W.; Sun, Tong; Collins, Stephen F.; Grattan, Kenneth T. V.

    2015-09-01

    An intensity ratio technique has been used for characterizing fluorescence spectra from novel coumarin dyes for pH sensing, in the range of 0.5 - 6, providing results that are independent of possible fluctuations in the intensity of the excitation source, deterioration of the indicator and changes in optical coupling. The arrangement was determined to have a sensitivity of 25% per unit pH change (at a pH of 4).

  2. Predicting Intrinsic mid-IR to optical flux ratios for galaxies of different types using Spectral Synthesis Models of Composite Stellar Populations

    NASA Astrophysics Data System (ADS)

    Kim, Duho; Jansen, Rolf A.; Windhorst, Rogier A.

    2016-01-01

    We analyze the intrinsic flux ratios of simple and composite stellar populations for various visible--near-infrared filters with respect to ˜3.5μm (L-band), and their dependence on metallicity, star-formation history, and effective mean age. This study is motivated by the fact that light from galaxies is reddened and attenuated by dust via scattering and absorption, where different sightlines across the face of a galaxy suffer various amounts of extinction. Ignoring the effects of this extinction could lead one to infer lower stellar mass, and SFR, or higher metallicity. Tamura et al. (2009) developed an approximate method, dubbed the "βV" method, which corrects for dust-extinction on a pixel-by-pixel basis, by comparing the observed flux ratio and empirical estimate of the intrinsic flux ratio of optical and ˜3.5μm broadband data. Here, we aim to validate and test the limits of the βV method for various filters spanning the visible through near-infrared wavelength range. Through extensive modeling, we test their assumptions for the intrinsic flux ratios for a wide variety of simple and composite stellar populations. We build spectral energy distributions (SEDs) of simple stellar populations (SSPs), by adopting Starburst99 and BC03 models for young (<9Myr) and old (>100Myr) stellar populations, respectively, and linear combinations of these for intermediate ages. We then construct composite stellar population (CSP) SEDs by combining SSP SEDs for various realistic star-formation histories (SFHs). We convolve filter response curves of visible--near-infrared filters for HST imaging surveys and mid-infrared filters in current (WISE, Spitzer/IRAC) and near-future use (JWST/NIRCam) with each model SED, to obtain intrinsic flux ratios (βλ,0). We find that βNIR,0 is only varying slightly as a function of metallicity but is insensitive to SFH or redshift (z≤2). We also find a narrow range of βV,0 (0.7+0.05-0.08) for early Hubble type galaxies (E and S0) using

  3. A new multifunction acousto-optic signal processor

    NASA Technical Reports Server (NTRS)

    Berg, N. J.; Casseday, M. W.; Filipov, A. N.; Pellegrino, J. M.

    1984-01-01

    An acousto-optic architecture for simultaneously obtaining time integration correlation and high-speed power spectrum analysis was constructed using commercially available TeO2 modulators and photodiode detector-arrays. The correlator section of the processor uses coherent interferometry to attain maximum bandwidth and dynamic range while achieving a time-bandwidth product of 1 million. Two correllator outputs are achieved in this system configuration. One is optically filtered and magnified 2 : 1 to decrease the spatial frequency to a level where a 25-MHz bandwidth may be sampled by a 62-mm array with elements on 25-micro centers. The other output is magnified by a factor of 10 such that the center 4 microseconds of information is available for estimation of time-difference-of-arrival to within 10 ns. The Bragg cell spectrum-analyzer section, which also has two outputs, resolves a 25-MHz instantaneous bandwidth to 25 kHz and can determine discrete-frequency reception time to within 15 microseconds. A microprocessor combines spectrum analysis information with that obtained from the correlator.

  4. No Evidence of Intrinsic Optical/Near-infrared Linear Polarization for V404 Cygni during Its Bright Outburst in 2015: Broadband Modeling and Constraint on Jet Parameters

    NASA Astrophysics Data System (ADS)

    Tanaka, Y. T.; Itoh, R.; Uemura, M.; Inoue, Y.; Cheung, C. C.; Watanabe, M.; Kawabata, K. S.; Fukazawa, Y.; Yatsu, Y.; Yoshii, T.; Tachibana, Y.; Fujiwara, T.; Saito, Y.; Kawai, N.; Kimura, M.; Isogai, K.; Kato, T.; Akitaya, H.; Kawabata, M.; Nakaoka, T.; Shiki, K.; Takaki, K.; Yoshida, M.; Imai, M.; Gouda, S.; Gouda, Y.; Akimoto, H.; Honda, S.; Hosoya, K.; Ikebe, A.; Morihana, K.; Ohshima, T.; Takagi, Y.; Takahashi, J.; Watanabe, K.; Kuroda, D.; Morokuma, T.; Murata, K.; Nagayama, T.; Nogami, D.; Oasa, Y.; Sekiguchi, K.

    2016-05-01

    We present simultaneous optical and near-infrared (NIR) polarimetric results for the black hole binary V404 Cyg spanning the duration of its seven-day-long optically brightest phase of its 2015 June outburst. The simultaneous R- and K s -band light curves showed almost the same temporal variation except for the isolated (˜30-minute duration) orphan K s -band flare observed at MJD 57193.54. We did not find any significant temporal variation of polarization degree (PD) and position angle (PA) in both R and K s bands throughout our observations, including the duration of the orphan NIR flare. We show that the observed PD and PA are predominantly interstellar in origin by comparing the V404 Cyg polarimetric results with those of the surrounding sources within the 7‧ × 7‧ field of view. The low intrinsic PD (less than a few percent) implies that the optical and NIR emissions are dominated by either disk or optically thick synchrotron emission, or both. We also present the broadband spectra of V404 Cyg during the orphan NIR flare and a relatively faint and steady state by including quasi-simultaneous Swift/XRT and INTEGRAL fluxes. By adopting a single-zone synchrotron plus inverse-Compton model as widely used in modeling of blazars, we constrained the parameters of a putative jet. Because the jet synchrotron component cannot exceed the Swift/XRT disk/corona flux, the cutoff Lorentz factor in the electron energy distribution is constrained to be <102, suggesting that particle acceleration is less efficient in this microquasar jet outburst compared to active galactic nucleus jets. We also suggest that the loading of the baryon component inside the jet is inevitable based on energetic arguments.

  5. Analog signal acquisition from computer optical disk drives for quantitative chemical sensing.

    PubMed

    Potyrailo, Radislav A; Morris, William G; Leach, Andrew M; Sivavec, Timothy M; Wisnudel, Marc B; Boyette, Scott

    2006-08-15

    Optoelectronic consumer products that are widely employed in the office and home attract attention for optical sensor applications due to (1) their cost advantage over analytical instruments produced only in small quantities, (2) robustness in operation due to the detailed manufacturability improvements, and (3) ease of operation. We demonstrate here a new approach for quantitative chemical/biochemical sensing when analog signals are acquired from conventional optical disk drives, and these signals are used for quantitative detection of optical changes of sensor films deposited on conventional CD and DVD optical disks. Because we do not alter manufacturing process of optical disks, any disk can be employed for deposition and readout of sensor films. The optical disk drives also perform their original function of reading and writing digital content to optical media because no optical modifications are introduced to obtain the analog signal. Such a sensor platform is quite universal and can be applied for chemical and biological quantitative detection, as well as for monitoring of changes of physical properties of regions deposited onto a CD or DVD (e.g., during combinatorial screening of materials). As a model example, we demonstrate the concept using chemical detection of ionic species such as Ca2+ in liquids (e.g., blood, urine, or water). Colorimetric calcium-sensitive sensor films were deposited onto a DVD, exposed to water with different concentrations of Ca2+, and quantified in the optical disk drive. The developed lab-on-DVD system demonstrated a 5 ppm detection limit of Ca2+ determinations, similar or slightly better than that achieved using a conventional fiber-optic portable spectrometer. This detection limit corresponded to a 0.023 absorbance unit resolution, as determined by the measurement of the same colorimetric films with a portable spectrometer. Determinations of Ca2+ unknowns using the lab-on-DVD system demonstrated +/-5 ppm accuracy and 2

  6. Signal contrast in coherent Raman scattering: Optical phonons versus biomolecules

    NASA Astrophysics Data System (ADS)

    Voronin, A. A.; Zheltikov, A. M.

    2012-09-01

    We show that the limiting contrast of the coherent anti-Stokes Raman scattering (CARS) signal with respect to the coherent background due to nonresonant four-wave mixing is controlled by the Q factor of the Raman mode and is independent of the parameters of laser pulses. High-Q phonon modes of semiconductor nanoparticles, such as diamond nanoprobes, can therefore substantially enhance the contrast of CARS images, as well as the sensitivity of CARS spectroscopy and microscopy compared to typical Raman-active vibrations of organic molecules in biotissues.

  7. Damage detection in composite materials using optical fibers: recent advances in signal processing

    NASA Astrophysics Data System (ADS)

    Staszewski, Wieslaw J.; Read, Ian J.; Foote, Peter D.

    2000-06-01

    This paper is concerned with the passive impact detection system based on fiber Bragg grating sensors which can be either embedded or surface mounted on a composite structure. The focus of the paper is the methodology of the intelligent signal processing for the optical fiber sensor data. This methodology is briefly discussed and illustrated using simple examples which utilize the experimental data. The experimental study involves a series of simple impact tests. The composite panel is installed in a loading fame. An instrumented impactor is used to damage the panel at different positions with different energy levels. For each impact the data from optical fiber sensors is digitized, logged and used for signal processing. The paper shows the importance of the intelligent signal processing for impact damage detection based on optical fiber sensors.

  8. Range-resolved interferometric signal processing using sinusoidal optical frequency modulation.

    PubMed

    Kissinger, Thomas; Charrett, Thomas O H; Tatam, Ralph P

    2015-04-01

    A novel signal processing technique using sinusoidal optical frequency modulation of an inexpensive continuous-wave laser diode source is proposed that allows highly linear interferometric phase measurements in a simple, self-referencing setup. Here, the use of a smooth window function is key to suppress unwanted signal components in the demodulation process. Signals from several interferometers with unequal optical path differences can be multiplexed, and, in contrast to prior work, the optical path differences are continuously variable, greatly increasing the practicality of the scheme. In this paper, the theory of the technique is presented, an experimental implementation using three multiplexed interferometers is demonstrated, and detailed investigations quantifying issues such as linearity and robustness against instrument drift are performed.

  9. Extracellular signal-regulated kinase pathway play distinct role in acetochlor-mediated toxicity and intrinsic apoptosis in A549 cells.

    PubMed

    Zerin, Tamanna; Song, Ho-Yeon; Kim, Yong-Sik

    2015-02-01

    Acetochlor (ACETO), a member of the chloroacetanilide family of herbicides, is widely used globally and is very frequently detected in watersheds of agricultural lands and fresh water streams. The human health consequences of environmental exposure to ACETO are unknown. This study was designed to elucidate the effect and molecular mechanisms of ACETO on human alveolar A549 cells. Established assays of cell viability and cytotoxicity were performed to detect the potential effects of ACETO on A549 cells. ACETO generated reactive oxygen species, which may have been crucial to apoptosis-mediated cytotoxicity. ACETO-treatment showed a concentration dependent up-regulation of pro-apoptotic proteins including Bax, Bak, BID and Bad, but a differential level of expression of anti-apoptotic proteins were observed, leading to the release of cytochrome c from mitochondria to the cytoplasm as well as activation of caspase-3, and cleavage of caspase-9 and PARP. ACETO also induced activation of extracellular signal-regulated kinase (ERK). Inhibition of the expression of ERK by PD98059 partially reversed ACETO-induced cytotoxicity, apoptosis and the expression of caspase-3, -9 and PARP in A549 cells. Comparative evaluation of the results indicates that the principal mechanism underlying ACETO-mediated cytotoxicity is likely to be through ERK-mediated intrinsic pathway of apoptosis. PMID:25291404

  10. Monitoring Ras Interactions with the Nucleotide Exchange Factor Son of Sevenless (Sos) Using Site-specific NMR Reporter Signals and Intrinsic Fluorescence*

    PubMed Central

    Vo, Uybach; Vajpai, Navratna; Flavell, Liz; Bobby, Romel; Breeze, Alexander L.; Embrey, Kevin J.; Golovanov, Alexander P.

    2016-01-01

    The activity of Ras is controlled by the interconversion between GTP- and GDP-bound forms partly regulated by the binding of the guanine nucleotide exchange factor Son of Sevenless (Sos). The details of Sos binding, leading to nucleotide exchange and subsequent dissociation of the complex, are not completely understood. Here, we used uniformly 15N-labeled Ras as well as [13C]methyl-Met,Ile-labeled Sos for observing site-specific details of Ras-Sos interactions in solution. Binding of various forms of Ras (loaded with GDP and mimics of GTP or nucleotide-free) at the allosteric and catalytic sites of Sos was comprehensively characterized by monitoring signal perturbations in the NMR spectra. The overall affinity of binding between these protein variants as well as their selected functional mutants was also investigated using intrinsic fluorescence. The data support a positive feedback activation of Sos by Ras·GTP with Ras·GTP binding as a substrate for the catalytic site of activated Sos more weakly than Ras·GDP, suggesting that Sos should actively promote unidirectional GDP → GTP exchange on Ras in preference of passive homonucleotide exchange. Ras·GDP weakly binds to the catalytic but not to the allosteric site of Sos. This confirms that Ras·GDP cannot properly activate Sos at the allosteric site. The novel site-specific assay described may be useful for design of drugs aimed at perturbing Ras-Sos interactions. PMID:26565026

  11. Monitoring Ras Interactions with the Nucleotide Exchange Factor Son of Sevenless (Sos) Using Site-specific NMR Reporter Signals and Intrinsic Fluorescence.

    PubMed

    Vo, Uybach; Vajpai, Navratna; Flavell, Liz; Bobby, Romel; Breeze, Alexander L; Embrey, Kevin J; Golovanov, Alexander P

    2016-01-22

    The activity of Ras is controlled by the interconversion between GTP- and GDP-bound forms partly regulated by the binding of the guanine nucleotide exchange factor Son of Sevenless (Sos). The details of Sos binding, leading to nucleotide exchange and subsequent dissociation of the complex, are not completely understood. Here, we used uniformly (15)N-labeled Ras as well as [(13)C]methyl-Met,Ile-labeled Sos for observing site-specific details of Ras-Sos interactions in solution. Binding of various forms of Ras (loaded with GDP and mimics of GTP or nucleotide-free) at the allosteric and catalytic sites of Sos was comprehensively characterized by monitoring signal perturbations in the NMR spectra. The overall affinity of binding between these protein variants as well as their selected functional mutants was also investigated using intrinsic fluorescence. The data support a positive feedback activation of Sos by Ras·GTP with Ras·GTP binding as a substrate for the catalytic site of activated Sos more weakly than Ras·GDP, suggesting that Sos should actively promote unidirectional GDP → GTP exchange on Ras in preference of passive homonucleotide exchange. Ras·GDP weakly binds to the catalytic but not to the allosteric site of Sos. This confirms that Ras·GDP cannot properly activate Sos at the allosteric site. The novel site-specific assay described may be useful for design of drugs aimed at perturbing Ras-Sos interactions.

  12. Asynchronous detection of optical code division multiple access signals using a bandwidth-efficient and wavelength-aware receiver.

    PubMed

    Fok, Mable P; Deng, Yanhua; Prucnal, Paul R

    2010-04-01

    We experimentally demonstrate what we believe to be a novel detection scheme for interfacing asynchronous optical code division multiple access (CDMA) signals with an electronic clock and data recovery system that operates only at the baseband bandwidth. This allows using a large optical bandwidth expansion factor in which the optical chip rate is much larger than the bandwidth of the optoelectronic receiver. The received optical CDMA signal is launched into a four-wave-mixing-based wavelength-aware all-optical front end that rejects multiaccess interference, followed by an amplitude-noise suppression stage comprised of a semiconductor optical amplifier. The clean signal is then converted into a non-return-to-zero-like signal by a baseband receiver. Using the proposed detection scheme, asynchronous transmission and detection of optical CDMA signals is implemented. With the novel detection scheme, the classic CDMA near-far problem is mitigated, and error-free detection is easily obtained.

  13. Kalman filtering to suppress spurious signals in Adaptive Optics control

    SciTech Connect

    Poyneer, L; Veran, J P

    2010-03-29

    In many scenarios, an Adaptive Optics (AO) control system operates in the presence of temporally non-white noise. We use a Kalman filter with a state space formulation that allows suppression of this colored noise, hence improving residual error over the case where the noise is assumed to be white. We demonstrate the effectiveness of this new filter in the case of the estimated Gemini Planet Imager tip-tilt environment, where there are both common-path and non-common path vibrations. We discuss how this same framework can also be used to suppress spatial aliasing during predictive wavefront control assuming frozen flow in a low-order AO system without a spatially filtered wavefront sensor, and present experimental measurements from Altair that clearly reveal these aliased components.

  14. Nonlinear Optical Microscopy Signal Processing Strategies in Cancer

    PubMed Central

    Adur, Javier; Carvalho, Hernandes F; Cesar, Carlos L; Casco, Víctor H

    2014-01-01

    This work reviews the most relevant present-day processing methods used to improve the accuracy of multimodal nonlinear images in the detection of epithelial cancer and the supporting stroma. Special emphasis has been placed on methods of non linear optical (NLO) microscopy image processing such as: second harmonic to autofluorescence ageing index of dermis (SAAID), tumor-associated collagen signatures (TACS), fast Fourier transform (FFT) analysis, and gray level co-occurrence matrix (GLCM)-based methods. These strategies are presented as a set of potential valuable diagnostic tools for early cancer detection. It may be proposed that the combination of NLO microscopy and informatics based image analysis approaches described in this review (all carried out on free software) may represent a powerful tool to investigate collagen organization and remodeling of extracellular matrix in carcinogenesis processes. PMID:24737930

  15. Advanced Silicon Microring Resonator Devices for Optical Signal Processing

    NASA Astrophysics Data System (ADS)

    Masilamani, Ashok Prabhu

    Chip level optical interconnects has gained momentum with recent demonstrations of silicon-on-insulator (SOI) based photonic modules such as lasers, modulators, wavelength division multiplexing (WDM) filters, etc. A fundamental building block that has enabled many of these silicon photonic modules is the compact, high Q factor microring resonator cavity. However, most of these demonstrations have WDM processing components based on simple add-drop filters that cannot realize the dense WDM systems required for the chip level interconnects. Dense WDM filters have stringent spectral shape requirements such as flat-top filter passband, steep band transition etc. Optical filters that can meet these specifications involve precise placement of the poles and zeros of the filter transfer function. Realization of such filters requires the use of multiple coupled microring resonators arranged in complex coupling topologies. In this thesis we have proposed and demonstrated new multiple coupled resonator topologies based on compact microring resonators in SOI material system. First we explored novel microring architectures which resulted in the proposal of two new coupled microring architectures, namely, the general 2D microring array topology and the general cascaded microring network topology. We also developed the synthesis procedures for these two microring architectures. The second part of this thesis focussed on the demonstration of the proposed architectures in the SOI material system. To accomplish this, a fabrication process for SOI was developed at the UofA Nanofab facility. Using this process, ultra-compact single microring filters with microring radii as small as 1mum were demonstrated. Higher order filter demonstration with multiple microrings necessitated post-fabrication microring resonance tuning. We developed additional fabrication steps to install micro heaters on top of the microrings to thermally tune its resonance. Subsequently, a thermally tuned fourth

  16. Compensation of chromatic and polarization mode dispersion in fiber-optic communication lines in microwave signals transmittion.

    NASA Astrophysics Data System (ADS)

    Ermolaev, A. N.; Krishpents, G. P.; Davydov, V. V.; Vysoczkiy, M. G.

    2016-08-01

    Methods of dispersion compensation in fiber-optic communication lines. A new proposed method of electronic dispersion compensation in the transmission of microwave signals through fiber-optic lines. Represents is proposed the results of experimental studies of this method.

  17. Confocal absorption spectral imaging of MoS2: optical transitions depending on the atomic thickness of intrinsic and chemically doped MoS2.

    PubMed

    Dhakal, Krishna P; Duong, Dinh Loc; Lee, Jubok; Nam, Honggi; Kim, Minsu; Kan, Min; Lee, Young Hee; Kim, Jeongyong

    2014-11-01

    We performed a nanoscale confocal absorption spectral imaging to obtain the full absorption spectra (over the range 1.5-3.2 eV) within regions having different numbers of layers and studied the variation of optical transition depending on the atomic thickness of the MoS2 film. Three distinct absorption bands corresponding to A and B excitons and a high-energy background (BG) peak at 2.84 eV displayed a gradual redshift as the MoS2 film thickness increased from the monolayer, to the bilayer, to the bulk MoS2 and this shift was attributed to the reduction of the gap energy in the Brillouin zone at the K-point as the atomic thickness increased. We also performed n-type chemical doping of MoS2 films using reduced benzyl viologen (BV) and the confocal absorption spectra modified by the doping showed a strong dependence on the atomic thickness: A and B exciton peaks were greatly quenched in the monolayer MoS2 while much less effect was shown in larger thickness and the BG peak either showed very small quenching for 1 L MoS2 or remained constant for larger thicknesses. Our results indicate that confocal absorption spectral imaging can provide comprehensive information on optical transitions of microscopic size intrinsic and doped two-dimensional layered materials.

  18. Quantum analysis of the nondegenerate optical parametric oscillator with injected signal

    SciTech Connect

    Coutinho dos Santos, B.; Dechoum, K.; Khoury, A.Z.; Silva, L.F. da; Olsen, M.K.

    2005-09-15

    In this paper we study the nondegenerate optical parametric oscillator with injected signal, both analytically and numerically. We develop a perturbation approach which allows us to find approximate analytical solutions, starting from the full equations of motion in the positive-P representation. We demonstrate the regimes of validity of our approximations via comparison with the full stochastic results. We find that, with reasonably low levels of injected signal, the system allows for demonstrations of quantum entanglement and the Einstein-Podolsky-Rosen paradox. In contrast to the normal optical parametric oscillator operating below threshold, these features are demonstrated with relatively intense fields.

  19. Genetically encoded optical probes for imaging cellular signaling pathways.

    PubMed

    Umezawa, Yoshio

    2005-06-15

    The intracellular signaling can be monitored in vivo in living cells by genetically encoded intracellular fluorescent and bioluminescent probes or indicators, which include second messengers, protein phosphorylation, protein conformational changes, protein-protein interactions, and protein localizations. These probes are of general use not only for fundamental biological studies, but also for assay and screening of possible pharmaceutical or toxic chemicals that inhibit or facilitate cellular signaling pathways. In this review, two examples of such indicators were briefly introduced. First, a genetically encoded fluorescent indicator was described for the detection and characterization of estrogen agonists and antagonists. The indicator was named SCCoR (single cell-coactivator recruitment). The high sensitivity of the present indicator made it possible to distinguish between estrogen strong and weak agonists in a dose-dependent fashion, immediately after adding a ligand to live cells. Discrimination of agonists from antagonists was efficiently achieved using the indicator. The approach described here can be applied to develop biosensors for other hormone receptors as well. Another example herein is a genetically encoded bioluminescent indicator for monitoring the nuclear trafficking of target proteins in vitro and in vivo. We demonstrated quantitative cell-based in vitro sensing of ligand-induced translocation of androgen receptor, which allowed high-throughput screening of exo- and endogenous agonists and antagonists. Furthermore, the indicator enabled noninvasive in vivo imaging of the androgen receptor translocation in the brains of living mice with a charge-coupled device imaging system. These rapid and quantitative analyses in vitro and in vivo provide a wide variety of applications for screening pharmacological or toxicological compounds and testing them in living animals.

  20. Stabilization of Phase of a Sinusoidal Signal Transmitted Over Optical Fiber

    NASA Technical Reports Server (NTRS)

    DAddario, Larry R.; Trink, Joseph T.

    2010-01-01

    In the process of connecting widely distributed antennas into a coherent array, it is necessary to synchronize the timing of signals at the various locations. This can be accomplished by distributing a common reference signal from a central source, usually over optical fiber. A high-frequency (RF or microwave) tone is a good choice for the reference. One difficulty is that the effective length of the optical fiber changes with temperature and mechanical stress, leading to phase instability in the received tone. This innovation provides a new way to stabilize the phase of the received tone, in spite of variations in the electrical length of the fiber. Stabilization is accomplished by two-way transmission in which part of the received signal is returned to the transmitting end over an identical fiber. The returned signal is detected and used to close an electrical servo loop whose effect is to keep constant the phase of the tone at the receiving end.

  1. Influence of large signal modulation on photonic UWB generation based on electro-optic modulator.

    PubMed

    Gu, Rong; Pan, Shilong; Chen, Xiangfei; Pan, Minghai; Ben, De

    2011-07-01

    Various schemes based on electro-optic modulators have been reported to generate ultra-wideband (UWB) signals in the optical domain, but the availability of these methods always relies on small signal modulation. In this paper, the influence of large signal modulation on two typical schemes, representing two major categories of external-modulator-based photonic UWB generation schemes, is analytically and numerically studied. While the quasi single-sideband UWB (QSSB-UWB) pulse can maintain its shape, the Gaussian UWB (GUWB) generation scheme suffers serious modulation distortion when the phase modulation index is greater than π/6. The modulation distortion would have negative impact on the receiver sensitivity when the signal is sent to a correlation receiver.

  2. Radio over fiber system carrying OFDM signal based on optical octuple frequency technique

    NASA Astrophysics Data System (ADS)

    Zhou, Hui; Zheng, Zhiwei; Wan, Qiuzhen

    2015-08-01

    We have proposed a scheme of radio over fiber (ROF) system with octuple optical millimeter-wave (mm-wave) generation transmit orthogonal frequency division multiplexing (OFDM) signal based on odd order sidebands suppression technique and filtering. The transmission performance of the optical mm-wave OFDM signal is theoretically investigated and implemented by numerical simulation. The results show that the time shift induced by chromatic dispersion only causes a slight phase rotation for constellation diagram, and a 40 GHz ROF system with a 2.5 Gbit/s signal remain has a good performance after the signal is transmitted over 50 km standard single-mode fiber (SSMF). Then the effects of the Mach-Zehnder modulator (MZM) DC-bias and modulation depth (MD) on the system performance are discussed by simulation, and the optimal performance of the system is obtained.

  3. High-frequency signal generation using 1550 nm VCSEL subject to two-frequency optical injection

    NASA Astrophysics Data System (ADS)

    Consoli, Antonio; Quirce, Ana; Valle, Angel; Esquivias, Ignacio; Pesquera, Luis; García Tijero, Jose Manuel

    2013-03-01

    We experimentally investigate high-frequency microwave signal generation using a 1550 nm single-mode VCSEL subject to two-frequency optical injection. We first consider a situation in which the injected signals come from two similar VCSELs. The polarization of the injected light is parallel to that of the injected VCSEL. We obtain that the VCSEL can be locked to one of the injected signals, but the observed microwave signal is originated by beating at the photodetector. In a second situation we consider injected signals that come from two external cavity tunable lasers with a significant increase of the injected power with respect to the VCSEL-by-VCSEL injection case. The polarization of the injected light is orthogonal to that of the free-running slave VCSEL. We show that in this case it is possible to generate a microwave signal inside the VCSEL cavity.

  4. Detection of radio-frequency modulated optical signals by two and three terminal microwave devices

    NASA Technical Reports Server (NTRS)

    Bhasin, K. B.; Simons, R. N.; Wojtczuk, S.

    1987-01-01

    An interdigitated photoconductor (two terminal device) on GaAlAs/GaAs heterostructure was fabricated and tested by an electro-optical sampling technique. Further, the photoresponse of GaAlAs/GaAs HEMT (three terminal device) was obtained by illuminating the device with an optical signal modulated up to 8 GHz. Gain-bandwidth product, response time, and noise properties of photoconductor and HEMT devices were obtained. Monolithic integration of these photodetectors with GaAs microwave devices for optically controlled phased array antenna applications is discussed.

  5. Wide bandwidth chaotic signal generation in a monolithically integrated semiconductor laser via optical injection

    NASA Astrophysics Data System (ADS)

    Yin, Xue-Mei; Zhong, Zhu-Qiang; Zhao, Ling-Juan; Lu, Dan; Qiu, Hai-Ying; Xia, Guang-Qiong; Wu, Zheng-Mao

    2015-11-01

    Wide bandwidth chaotic signal generation in a three-section monolithically integrated semiconductor laser (MISL) under external optical injection is investigated experimentally. Through evaluating the effective bandwidth of chaotic signals, the influences of the optical injection on the bandwidth of chaotic signal from the MISL are analyzed. The experimental results indicate that, for the currents of the DFB section (IDFB) and the phase section (IP) are fixed at 70.00 mA and 34.00 mA, respectively, the effective bandwidth of chaos signal generated by the solitary MISL reaches its maximum value of 14.36 GHz when the current of the amplification section (IA) takes 23.22 mA. After an external optical injection is introduced into the MISL, the effective bandwidth of the generated chaotic signal can be beyond 2.5 times of the maximum value. Furthermore, the effects of the injection strength and the frequency detuning on the effective bandwidth of the generated chaotic signal are also discussed.

  6. To-and-fro optical voltage signal propagation between the insular gustatory and parietal oral somatosensory areas in rat cortex slices.

    PubMed

    Yoshimura, Hiroshi; Kato, Nobuo; Sugai, Tokio; Honjo, Makoto; Sato, Jun; Segami, Natsuki; Onoda, Norihiko

    2004-07-23

    Taste perception depends not only on special taste information processed in the insular cortex, but also on oral somesthetic processing in the parietal cortex. Many insular cortex neurons show multimodal responsiveness. Such multimodality may be enabled by signal exchange between these two cortices. By using the protocol that we have developed, a synchronized population oscillation of synaptic potentials was induced in the parietal cortex by stimulation to the insular cortex in rat neocortex slices. The spatiotemporal pattern of propagation of this oscillation was studied by recording voltage-sensitive optical signals and field potentials. The first wavelet of the oscillation was propagated from the insular stimulation site to the parietal cortex. However, the second and later wavelets propagated back from the parietal cortex to the insular cortex. The oscillation was detected in the insular cortex as well, but was actually generated in the parietal cortex. Thus, the initial peak of optical signal, sent from the insular to parietal cortex, served to generate oscillatory responses in the parietal cortex, which propagated back to the insular cortex wave-by-wave. We propose that this to-and-fro propagation may be an artificially exaggerated demonstration of an intrinsic mechanism relevant to signal exchange between the parietal and insular cortices.

  7. The Biases of Optical Line-Ratio Selection for Active Galactic Nuclei and the Intrinsic Relationship between Black Hole Accretion and Galaxy Star Formation

    NASA Astrophysics Data System (ADS)

    Trump, Jonathan R.; Sun, Mouyuan; Zeimann, Gregory R.; Luck, Cuyler; Bridge, Joanna S.; Grier, Catherine J.; Hagen, Alex; Juneau, Stephanie; Montero-Dorta, Antonio; Rosario, David J.; Brandt, W. Niel; Ciardullo, Robin; Schneider, Donald P.

    2015-09-01

    We use 317,000 emission-line galaxies from the Sloan Digital Sky Survey to investigate line-ratio selection of active galactic nuclei (AGNs). In particular, we demonstrate that “star formation (SF) dilution” by H ii regions causes a significant bias against AGN selection in low-mass, blue, star-forming, disk-dominated galaxies. This bias is responsible for the observed preference of AGNs among high-mass, green, moderately star-forming, bulge-dominated hosts. We account for the bias and simulate the intrinsic population of emission-line AGNs using a physically motivated Eddington ratio distribution, intrinsic AGN narrow line region line ratios, a luminosity-dependent {L}{bol}/L[{{O}} {{III}}] bolometric correction, and the observed {M}{BH}-σ relation. These simulations indicate that, in massive ({log}({M}*/{M}⊙ )≳ 10) galaxies, AGN accretion is correlated with specific star formation rate (SFR) but is otherwise uniform with stellar mass. There is some hint of lower black hole occupation in low-mass ({log}({M}*/{M}⊙ )≲ 10) hosts, although our modeling is limited by uncertainties in measuring and interpreting the velocity dispersions of low-mass galaxies. The presence of SF dilution means that AGNs contribute little to the observed strong optical emission lines (e.g., [{{O}} {{III}}] and {{H}}α ) in low-mass and star-forming hosts. However the AGN population recovered by our modeling indicates that feedback by typical (low- to moderate-accretion) low-redshift AGNs has nearly uniform efficiency at all stellar masses, SFRs, and morphologies. Taken together, our characterization of the observational bias and resultant AGN occupation function suggest that AGNs are unlikely to be the dominant source of SF quenching in galaxies, but instead are fueled by the same gas which drives SF activity.

  8. Optical coherence tomography for blood glucose monitoring through signal attenuation

    NASA Astrophysics Data System (ADS)

    De Pretto, Lucas R.; Yoshimura, Tania M.; Ribeiro, Martha S.; de Freitas, Anderson Z.

    2016-03-01

    Development of non-invasive techniques for glucose monitoring is crucial to improve glucose control and treatment adherence in patients with diabetes. Hereafter, Optical Coherence Tomography (OCT) may offer a good alternative for portable glucometers, since it uses light to probe samples. Changes in the object of interest can alter the intensity of light returning from the sample and, through it, one can estimate the sample's attenuation coefficient (μt) of light. In this work, we aimed to explore the behavior of μt of mouse's blood under increasing glucose concentrations. Different samples were prepared in four glucose concentrations using a mixture of heparinized blood, phosphate buffer saline and glucose. Blood glucose concentrations were measured with a blood glucometer, for reference. We have also prepared other samples diluting the blood in isotonic saline solution to check the effect of a higher multiple-scattering component on the ability of the technique to differentiate glucose levels based on μt. The OCT system used was a commercial Spectral Radar OCT with 930 nm central wavelength and spectral bandwidth (FWHM) of 100 nm. The system proved to be sensitive for all blood glucose concentrations tested, with good correlations with the obtained attenuation coefficients. A linear tendency was observed, with an increase in attenuation with higher values of glucose. Statistical difference was observed between all groups (p<0.001). This work opens the possibility towards a non-invasive diagnostic modality using OCT for glycemic control, which eliminates the use of analytes and/or test strips, as in the case with commercially available glucometers.

  9. Influence of optical gaps on signal and noise properties of luminescent screen x-ray detectors

    NASA Astrophysics Data System (ADS)

    Koch, Andreas

    2004-05-01

    X-ray detection with luminescent screens requires optical signal transfer as an intermediate step between x-ray detection and conversion to an electronic signal. Luminescent screens may be granular (phosphor screens), structured (e.g. CsI) or transparent (scintillators). The optical signal is imaged with lenses, fibre optics, electron optics or by proximity focussing to an electronic detector. Poor focussing or poor optical contact may degrade the signal and noise transfer characteristics, i.e. modulation transfer function (MTF) and detective quantum efficiency (DQE). The case when x-rays are detected with granular luminescent screens, imaged onto flat panel electronic detectors is considered here. The detector assembly often requires layers of glue or protective thin films creating optical gaps, in which light is spread, hence spatial resolution is degraded. The noise spectrum is not necessarily changed the same way. Its exact shape depends on the dominant noise sources in a given detector configuration under the specific operating conditions: The noise of the primary x-ray quanta, noise aliasing and direct x-ray detection by the electronic detection layer are the main contributions in this investigation. Especially at high spatial frequencies small optical gaps in conjunction with white quantum noise from direct x-ray absorption of the electronic imager degrade DQE: A gap of 40 μm between luminescent screen and detector reduces the DQE by 33% at the Nyquist frequency. This was demonstrated with an a-Si imager of 143-μm pixel size and a Lanex Fine luminescent screen operated at 100 kV.

  10. Functional organization of visual cortex in the prosimian bush baby revealed by optical imaging of intrinsic signals.

    PubMed

    Xu, Xiangmin; Bosking, William H; White, Leonard E; Fitzpatrick, David; Casagrande, Vivien A

    2005-10-01

    Cells in primary visual cortex (V1) of primates and carnivores respond most strongly to a visual stimulus presented to one eye, in a particular visual field location, and at a particular orientation. Each of these stimulus attributes is mapped across the cortical surface, and, in macaque monkeys and cats, strong geometrical relationships exist between these feature maps. In macaque V1 and V2, correlations between feature maps and cytochrome oxidase (CO)-rich modules have also been observed. To see if such relationships reflect a conserved principle of V1 functional architecture among primate species, we examined these maps in the prosimian bush baby, a species that has been proposed to represent the ancestral primate organization. We found that the layout of individual feature maps in bush baby V1 is similar to that of other primates, but we found an entirely different organization of orientation preference in bush baby V2 compared with that reported in simian primates. Another striking distinction between bush baby and simian species is that we observed no strong relationships among maps of orientation, ocular dominance, and CO blobs in V1. Thus our findings suggest that precise relationships between feature maps are not a common element of the functional organization in all primates and that such relationships are not necessary for achieving basic coverage of stimulus feature combinations. In addition, our results suggest that specific relationships between feature maps in V1, and the subdivision of V2 into functional compartments, may have arisen comparatively late in the evolution of primates. PMID:16000523

  11. Combined optical-electrical finite-element simulations of thin-film solar cells with homogeneous and nonhomogeneous intrinsic layers

    NASA Astrophysics Data System (ADS)

    Anderson, Tom H.; Faryad, Muhammad; Mackay, Tom G.; Lakhtakia, Akhlesh; Singh, Rajendra

    2016-04-01

    A two-dimensional finite-element model was developed to simulate the optoelectronic performance of thin-film, p-i-n junction solar cells. One or three p-i-n junctions filled the region between the front window and back reflector; semiconductor layers were made from mixtures of two different alloys of hydrogenated amorphous silicon; empirical relationships between the complex-valued relative optical permittivity and the bandgap were used; a transparent-conducting-oxide layer was attached to the front surface of the solar cell; and a metallic reflector, either flat or periodically corrugated, was attached to the back surface. First, frequency-domain Maxwell postulates were solved to determine the spatial absorption of photons and thus the generation of electron-hole pairs. The AM1.5G solar spectrum was taken to represent the incident solar flux. Second, drift-diffusion equations were solved for the steady-state electron and hole densities. Numerical results indicate that increasing the number of p-i-n junctions from one to three may increase the solar-cell efficiency by up to 14%. In the case of single p-i-n junction solar cells, our simulations indicate that efficiency may be increased by up to 17% by incorporating a periodically corrugated back reflector (as opposed to a flat back reflector) and by tailoring the bandgap profile in the i layer.

  12. Ultralow-phase-noise millimetre-wave signal generator assisted with an electro-optics-modulator-based optical frequency comb

    NASA Astrophysics Data System (ADS)

    Ishizawa, A.; Nishikawa, T.; Goto, T.; Hitachi, K.; Sogawa, T.; Gotoh, H.

    2016-05-01

    Low-noise millimetre-wave signals are valuable for digital sampling systems, arbitrary waveform generation for ultra-wideband communications, and coherent radar systems. However, the phase noise of widely used conventional signal generators (SGs) will increase as the millimetre-wave frequency increases. Our goal has been to improve commercially available SGs so that they provide a low-phase-noise millimetre-wave signal with assistance from an electro-optics-modulator-based optical frequency comb (EOM-OFC). Here, we show that the phase noise can be greatly reduced by bridging the vast frequency difference between the gigahertz and terahertz ranges with an EOM-OFC. The EOM-OFC serves as a liaison that magnifies the phase noise of the SG. With the EOM-OFC used as a phase noise “booster” for a millimetre-wave signal, the phase noise of widely used SGs can be reduced at an arbitrary frequency f (6 ≦ f ≦ 72 GHz).

  13. Ultralow-phase-noise millimetre-wave signal generator assisted with an electro-optics-modulator-based optical frequency comb

    PubMed Central

    Ishizawa, A.; Nishikawa, T.; Goto, T.; Hitachi, K.; Sogawa, T.; Gotoh, H.

    2016-01-01

    Low-noise millimetre-wave signals are valuable for digital sampling systems, arbitrary waveform generation for ultra-wideband communications, and coherent radar systems. However, the phase noise of widely used conventional signal generators (SGs) will increase as the millimetre-wave frequency increases. Our goal has been to improve commercially available SGs so that they provide a low-phase-noise millimetre-wave signal with assistance from an electro-optics-modulator-based optical frequency comb (EOM-OFC). Here, we show that the phase noise can be greatly reduced by bridging the vast frequency difference between the gigahertz and terahertz ranges with an EOM-OFC. The EOM-OFC serves as a liaison that magnifies the phase noise of the SG. With the EOM-OFC used as a phase noise “booster” for a millimetre-wave signal, the phase noise of widely used SGs can be reduced at an arbitrary frequency f (6 ≦ f ≦ 72 GHz). PMID:27185040

  14. Ultralow-phase-noise millimetre-wave signal generator assisted with an electro-optics-modulator-based optical frequency comb.

    PubMed

    Ishizawa, A; Nishikawa, T; Goto, T; Hitachi, K; Sogawa, T; Gotoh, H

    2016-01-01

    Low-noise millimetre-wave signals are valuable for digital sampling systems, arbitrary waveform generation for ultra-wideband communications, and coherent radar systems. However, the phase noise of widely used conventional signal generators (SGs) will increase as the millimetre-wave frequency increases. Our goal has been to improve commercially available SGs so that they provide a low-phase-noise millimetre-wave signal with assistance from an electro-optics-modulator-based optical frequency comb (EOM-OFC). Here, we show that the phase noise can be greatly reduced by bridging the vast frequency difference between the gigahertz and terahertz ranges with an EOM-OFC. The EOM-OFC serves as a liaison that magnifies the phase noise of the SG. With the EOM-OFC used as a phase noise "booster" for a millimetre-wave signal, the phase noise of widely used SGs can be reduced at an arbitrary frequency f (6 ≦ f ≦ 72 GHz). PMID:27185040

  15. Code extraction from encoded signal in time-spreading optical code division multiple access.

    PubMed

    Si, Zhijian; Yin, Feifei; Xin, Ming; Chen, Hongwei; Chen, Minghua; Xie, Shizhong

    2010-01-15

    A vulnerability that allows eavesdroppers to extract the code from the waveform of the noiselike encoded signal of an isolated user in a standard time-spreading optical code division multiple access communication system using bipolar phase code is experimentally demonstrated. The principle is based on fine structure in the encoded signal. Each dip in the waveform corresponds to a transition of the bipolar code. Eavesdroppers can get the code by analyzing the chip numbers between any two transitions; then a decoder identical to the legal user's can be fabricated, and they can get the properly decoded signal.

  16. Millimeter-wave and microwave signal generation by low-bandwidth electro-optic phase modulation.

    PubMed

    Torres-Company, Víctor; Fernández-Alonso, Mercedes; Lancis, Jesús; Barreiro, Juan C; Andrés, Pedro

    2006-10-16

    We propose, analyze and numerically illustrate a photonic-based technique for waveform generation of electrical signals approaching the 50 GHz bandwidth with time apertures as large as a few nanoseconds, by low-frequency, up to 2 GHz, electro-optic phase modulation of time-stretched optical pulses. Synthesis of the electrical waveform relies on phase-to-amplitude conversion of the modulated signal by a group delay dispersion circuit designed to behave as a transversal filter with N taps. Although arbitrary waveform generation capabilities are limited, a wide variety of user-defined signals are numerically demonstrated by appropriately designing the low-frequency signal driving the electro-optical modulator. Frequency upshifting is controlled by the chirp of the stretched pulse which provides an additional degree of freedom. Finally, optical-to-electrical conversion allows for the user-defined electrical waveform. Simulations are given for square waveform generation demonstrating the high resolution and wide-band capabilities of the technique.

  17. Multiplexing free-space optical signals using superimposed collinear orbital angular momentum states

    NASA Astrophysics Data System (ADS)

    Lin, J.; Yuan, X.-C.; Tao, S. H.; Burge, R. E.

    2007-07-01

    As a proof of concept, we experimentally demonstrate multiplexing of free-space optical signals in multiple channels labeled with different states of orbital angular momentum. The multiplexing process is carried out by a dynamic liquid-crystal spatial light modulator, while the phase function is calculated by an iterative algorithm. A binary amplitude computer-generated hologram serves as a demultiplexer.

  18. Analysis of the measured signals in apertureless near-field optical microscopy.

    PubMed

    Formanek, F; De Wilde, Y; Aigouy, L

    2005-05-01

    We present an analytical model able to explain the optical signal recorded during our experimental approach curves in the infrared at a wavelength lambda=10.6 microm, with a home-made apertureless near-field scanning optical microscope ANSOM. This model uses classical electrodynamics to calculate the scattering cross section of the oscillating tip, considered as a dipole, and its dielectric image in the sample as a function of the tip-sample separation from the near-field to the far-field regime. The dipoles are placed in a non-uniform electric field because of the standing wave arising from the interference between the incident and the specular laser beams. We also added a background field coming from a scatterer on the surface in order to account for zeroing of the optical signal for particular tip-sample separation and interference patterns.

  19. Adaptive coded spreading OFDM signal for dynamic-λ optical access network

    NASA Astrophysics Data System (ADS)

    Liu, Bo; Zhang, Lijia; Xin, Xiangjun

    2015-12-01

    This paper proposes and experimentally demonstrates a novel adaptive coded spreading (ACS) orthogonal frequency division multiplexing (OFDM) signal for dynamic distributed optical ring-based access network. The wavelength can be assigned to different remote nodes (RNs) according to the traffic demand of optical network unit (ONU). The ACS can provide dynamic spreading gain to different signals according to the split ratio or transmission length, which offers flexible power budget for the network. A 10×13.12 Gb/s OFDM access with ACS is successfully demonstrated over two RNs and 120 km transmission in the experiment. The demonstrated method may be viewed as one promising for future optical metro access network.

  20. Stimulated Brillouin scattering gain bandwidth reduction and applications in microwave photonics and optical signal processing

    NASA Astrophysics Data System (ADS)

    Preussler, Stefan; Schneider, Thomas

    2016-03-01

    Stimulated Brillouin scattering (SBS) is one of the most dominant nonlinear effects in standard single-mode fibers and its unique spectral characteristics, especially the narrow bandwidth, enable many different applications. Most of the applications would benefit from a narrower bandwidth. Different methods for the bandwidth reduction of SBS in optical fibers are presented and discussed. A bandwidth reduction down to 17% of the natural gain can be achieved by the superposition of the gain with two losses or the utilization of a multistage system. Furthermore, applications in the field of microwave photonics and optical signal processing like high-resolution spectroscopy of communication signals, the storage of optical data packets as well as the processing of frequency combs including generation of millimeter waves and ideal sinc-shaped Nyquist pulses are presented.

  1. Gold nanoparticle-assisted all optical localized stimulation and monitoring of Ca2+ signaling in neurons

    PubMed Central

    Lavoie-Cardinal, Flavie; Salesse, Charleen; Bergeron, Éric; Meunier, Michel; De Koninck, Paul

    2016-01-01

    Light-assisted manipulation of cells to control membrane activity or intracellular signaling has become a major avenue in life sciences. However, the ability to perform subcellular light stimulation to investigate localized signaling has been limited. Here, we introduce an all optical method for the stimulation and the monitoring of localized Ca2+ signaling in neurons that takes advantage of plasmonic excitation of gold nanoparticles (AuNPs). We show with confocal microscopy that 800 nm laser pulse application onto a neuron decorated with a few AuNPs triggers a transient increase in free Ca2+, measured optically with GCaMP6s. We show that action potentials, measured electrophysiologically, can be induced with this approach. We demonstrate activation of local Ca2+ transients and Ca2+ signaling via CaMKII in dendritic domains, by illuminating a single or few functionalized AuNPs specifically targeting genetically-modified neurons. This NP-Assisted Localized Optical Stimulation (NALOS) provides a new complement to light-dependent methods for controlling neuronal activity and cell signaling. PMID:26857748

  2. Measurement of splanchnic photoplethysmographic signals using a new reflectance fiber optic sensor

    NASA Astrophysics Data System (ADS)

    Hickey, Michelle; Samuels, Neal; Randive, Nilesh; Langford, Richard M.; Kyriacou, Panayiotis A.

    2010-03-01

    Splanchnic organs are particularly vulnerable to hypoperfusion. Currently, there is no technique that allows for the continuous estimation of splanchnic blood oxygen saturation (SpO2). As a preliminary to developing a suitable splanchnic SpO2 sensor, a new reflectance fiber optic photoplethysmographic (PPG) sensor and processing system are developed. An experimental procedure to examine the effect of fiber source detector separation distance on acquired PPG signals is carried out before finalizing the sensor design. PPG signals are acquired from four volunteers for separation distances of 1 to 8 mm. The separation range of 3 to 6 mm provides the best quality PPG signals with large amplitudes and the highest signal-to-noise ratios (SNRs). Preliminary calculation of SpO2 shows that distances of 3 and 4 mm provide the most realistic values. Therefore, it is suggested that the separation distance in the design of a fiber optic reflectance pulse oximeter be in the range of 3 to 4 mm. Preliminary PPG signals from various splanchnic organs and the periphery are obtained from six anaesthetized patients. The normalized amplitudes of the splanchnic PPGs are, on average, approximately the same as those obtained simultaneously from the periphery. These observations suggest that fiber optic pulse oximetry may be a valid monitoring technique for splanchnic organs.

  3. ``Seeing'' electroencephalogram through the skull: imaging prefrontal cortex with fast optical signal

    NASA Astrophysics Data System (ADS)

    Medvedev, Andrei V.; Kainerstorfer, Jana M.; Borisov, Sergey V.; Gandjbakhche, Amir H.; Vanmeter, John

    2010-11-01

    Near-infrared spectroscopy is a novel imaging technique potentially sensitive to both brain hemodynamics (slow signal) and neuronal activity (fast optical signal, FOS). The big challenge of measuring FOS noninvasively lies in the presumably low signal-to-noise ratio. Thus, detectability of the FOS has been controversially discussed. We present reliable detection of FOS from 11 individuals concurrently with electroencephalogram (EEG) during a Go-NoGo task. Probes were placed bilaterally over prefrontal cortex. Independent component analysis (ICA) was used for artifact removal. Correlation coefficient in the best correlated FOS-EEG ICA pairs was highly significant (p < 10-8), and event-related optical signal (EROS) was found in all subjects. Several EROS components were similar to the event-related potential (ERP) components. The most robust ``optical N200'' at t = 225 ms coincided with the N200 ERP; both signals showed significant difference between targets and nontargets, and their timing correlated with subject's reaction time. Correlation between FOS and EEG even in single trials provides further evidence that at least some FOS components ``reflect'' electrical brain processes directly. The data provide evidence for the early involvement of prefrontal cortex in rapid object recognition. EROS is highly localized and can provide cost-effective imaging tools for cortical mapping of cognitive processes.

  4. Gold nanoparticle-assisted all optical localized stimulation and monitoring of Ca2+ signaling in neurons

    NASA Astrophysics Data System (ADS)

    Lavoie-Cardinal, Flavie; Salesse, Charleen; Bergeron, Éric; Meunier, Michel; de Koninck, Paul

    2016-02-01

    Light-assisted manipulation of cells to control membrane activity or intracellular signaling has become a major avenue in life sciences. However, the ability to perform subcellular light stimulation to investigate localized signaling has been limited. Here, we introduce an all optical method for the stimulation and the monitoring of localized Ca2+ signaling in neurons that takes advantage of plasmonic excitation of gold nanoparticles (AuNPs). We show with confocal microscopy that 800 nm laser pulse application onto a neuron decorated with a few AuNPs triggers a transient increase in free Ca2+, measured optically with GCaMP6s. We show that action potentials, measured electrophysiologically, can be induced with this approach. We demonstrate activation of local Ca2+ transients and Ca2+ signaling via CaMKII in dendritic domains, by illuminating a single or few functionalized AuNPs specifically targeting genetically-modified neurons. This NP-Assisted Localized Optical Stimulation (NALOS) provides a new complement to light-dependent methods for controlling neuronal activity and cell signaling.

  5. An all-fiber partial discharge monitoring system based on both intrinsic fiber optic interferometry sensor and fluorescent fiber

    NASA Astrophysics Data System (ADS)

    Yin, Zelin; Zhang, Ruirui; Tong, Jie; Chen, Xi

    2013-12-01

    Partial discharges (PDs) are an electrical phenomenon that occurs within a transformer whenever the voltage stress is sufficient to produce ionization in voids or inclusions within a solid dielectric, at conductor/dielectric interfaces, or in bubbles within liquid dielectrics such as oil; high-frequency transient current discharges will then appear repeatedly and will progressively deteriorate the insulation, ultimately leading to breakdown. Fiber sensor has great potential on the partial discharge detection in high-voltage equipment for its immunity to electromagnetic interference and it can take direct measurement in the high voltage equipment. The energy released in PDs produces a number of effects, resulting in flash, chemical and structural changes and electromagnetic emissions and so on. Acoustic PD detection is based on the mechanical pressure wave emitted from the discharge and fluorescent fiber PD detection is based on the emitted light produced by ionization, excitation and recombination processes during the discharge. Both of the two methods have the shortage of weak anti-interference capacity in the physical environment, like thunder or other sound source. In order to avoid the false report, an all-fiber combined PD detection system of the two methods is developed in this paper. In the system the fluorescent fiber PD sensor is considered as a reference signal, three F-P based PD detection sensors are used to both monitor the PD intensity and calculate the exact position of the discharge source. Considering the wave band of the F-P cavity and the fluorescent probe are quite different, the reflection spectrum of the F-P cavity is in the infrared region, however the fluorescent probe is about 600nm to 700nm, thus the F-P sensor and fluorescent fiber probe can be connected in one fiber and the reflection light can be detected by two different detectors without mutual interference. The all-fiber partial discharge monitoring system not only can detect the PDs

  6. Tailoring Vacancies Far Beyond Intrinsic Levels Changes the Carrier Type and Optical Response in Monolayer MoSe2-x Crystals.

    PubMed

    Mahjouri-Samani, Masoud; Liang, Liangbo; Oyedele, Akinola; Kim, Yong-Sung; Tian, Mengkun; Cross, Nicholas; Wang, Kai; Lin, Ming-Wei; Boulesbaa, Abdelaziz; Rouleau, Christopher M; Puretzky, Alexander A; Xiao, Kai; Yoon, Mina; Eres, Gyula; Duscher, Gerd; Sumpter, Bobby G; Geohegan, David B

    2016-08-10

    Defect engineering has been a critical step in controlling the transport characteristics of electronic devices, and the ability to create, tune, and annihilate defects is essential to enable the range of next-generation devices. Whereas defect formation has been well-demonstrated in three-dimensional semiconductors, similar exploration of the heterogeneity in atomically thin two-dimensional semiconductors and the link between their atomic structures, defects, and properties has not yet been extensively studied. Here, we demonstrate the growth of MoSe2-x single crystals with selenium (Se) vacancies far beyond intrinsic levels, up to ∼20%, that exhibit a remarkable transition in electrical transport properties from n- to p-type character with increasing Se vacancy concentration. A new defect-activated phonon band at ∼250 cm(-1) appears, and the A1g Raman characteristic mode at 240 cm(-1) softens toward ∼230 cm(-1) which serves as a fingerprint of vacancy concentration in the crystals. We show that post-selenization using pulsed laser evaporated Se atoms can repair Se-vacant sites to nearly recover the properties of the pristine crystals. First-principles calculations reveal the underlying mechanisms for the corresponding vacancy-induced electrical and optical transitions.

  7. Tailoring Vacancies Far Beyond Intrinsic Levels Changes the Carrier Type and Optical Response in Monolayer MoSe 2-x Crystals

    DOE PAGES

    Mahjouri-Samani, Masoud; Liang, Liangbo; Oyedele, Akinola; Kim, Yong-Sung; Tian, Mengkun; Cross, Nicholas; Wang, Kai; Lin, Ming-Wei; Boulesbaa, Abdelaziz; Rouleau, Christopher M.; et al

    2016-07-14

    Defect engineering has been a critical step in controlling the transport characteristics of electronic devices, and the ability to create, tune, and annihilate defects is essential to enable the range of next-generation devices. Whereas defect formation has been well-demonstrated in three-dimensional semiconductors, similar exploration of the heterogeneity in atomically thin two-dimensional semiconductors and the link between their atomic structures, defects, and properties has not yet been extensively studied. In this paper, we demonstrate the growth of MoSe2–x single crystals with selenium (Se) vacancies far beyond intrinsic levels, up to ~20%, that exhibit a remarkable transition in electrical transport properties frommore » n- to p-type character with increasing Se vacancy concentration. A new defect-activated phonon band at ~250 cm-1 appears, and the A1g Raman characteristic mode at 240 cm-1 softens toward ~230 cm-1 which serves as a fingerprint of vacancy concentration in the crystals. We show that post-selenization using pulsed laser evaporated Se atoms can repair Se-vacant sites to nearly recover the properties of the pristine crystals. Finally, first-principles calculations reveal the underlying mechanisms for the corresponding vacancy-induced electrical and optical transitions.« less

  8. Cooperative transients in inter-atomic correlation in the presence of an externally applied coherent field - Relation to intrinsic mirrorless optical bistability

    NASA Astrophysics Data System (ADS)

    Bowden, C. M.; Sung, C. C.

    1982-08-01

    The model presented earlier (Bowden and Sung, 1979), which predicts the circumstances under which intrinsic mirrorless optical bistability (OB) can occur due to atomic pair correlation in a small volume, is outlined and the results presented. These results, which predict a first-order phase transition in steady state for an externally driven collection of a large number of atoms far removed from thermodynamic equilibrium, form the motivation for a detailed microscopic examination of the dynamical behavior of atomic pair correlation in the presence of externally applied coherent radiation. A model is presented and results are discussed for the transient dynamic evolution of two two-level atoms separated from each other by a distance r in the presence of an externally applied coherent radiation field. The results predict collective radiation reaction, frequency shifts, relaxation in terms of the atomic separation r (assumed much larger than single atom dimensions), the externally applied field intensity and spacial uniformity of the field with respect to the inter-atomic volume.

  9. Optical Correlation of Images With Signal-Dependent Noise Using Constrained-Modulation Filter Devices

    NASA Technical Reports Server (NTRS)

    Downie, John D.

    1995-01-01

    Images with signal-dependent noise present challenges beyond those of images with additive white or colored signal-independent noise in terms of designing the optimal 4-f correlation filter that maximizes correlation-peak signal-to-noise ratio, or combinations of correlation-peak metrics. Determining the proper design becomes more difficult when the filter is to be implemented on a constrained-modulation spatial light modulator device. The design issues involved for updatable optical filters for images with signal-dependent film-grain noise and speckle noise are examined. It is shown that although design of the optimal linear filter in the Fourier domain is impossible for images with signal-dependent noise, proper nonlinear preprocessing of the images allows the application of previously developed design rules for optimal filters to be implemented on constrained-modulation devices. Thus the nonlinear preprocessing becomes necessary for correlation in optical systems with current spatial light modulator technology. These results are illustrated with computer simulations of images with signal-dependent noise correlated with binary-phase-only filters and ternary-phase-amplitude filters.

  10. Zinc ferrite nanoparticles activate IL-1b, NFKB1, CCL21 and NOS2 signaling to induce mitochondrial dependent intrinsic apoptotic pathway in WISH cells

    SciTech Connect

    Saquib, Quaiser; Al-Khedhairy, Abdulaziz A.; Ahmad, Javed; Siddiqui, Maqsood A.; Dwivedi, Sourabh; Khan, Shams T.; Musarrat, Javed

    2013-12-01

    localization of NPs. • ZnFe{sub 2}O{sub 4}-NPs induce DNA damage and mitochondrial dysfunction in WISH cells. • ZnFe{sub 2}O{sub 4}-NPs activate inflammatory and oxidative stress signaling in WISH cells. • Elevation of p53, CASP 3, bax and bcl 2 genes affirms intrinsic apoptotic pathway.

  11. Microwave photonic filter with reconfigurable and tunable bandpass response using integrated optical signal processor based on microring resonator

    NASA Astrophysics Data System (ADS)

    Zhang, Zan; Huang, Beiju; Zhang, Zanyun; Cheng, Chuantong; Chen, Hongda

    2013-12-01

    A bandpass microwave photonic filter based on an integrated optical signal processor is proposed and demonstrated by numerical simulation. The optical signal processor consisting of double-bus-coupled and series-cascaded silicon microrings (MRs) is used to produce two bandpass responses to process optical carrier signal and sideband signal separately. Because of the tunability of MRs, variable -3 dB bandwidth and tunable operating frequency are achieved. The -3 dB bandwidth and operating frequency can be tuned from 1.5 to 12 GHz and from 15 to 34 GHz, respectively. The loss impact, tuning method, and fabrication error tolerance are also discussed.

  12. Asynchronous, all-optical signal processing based on the self-frequency shift of a gigahertz Raman soliton.

    PubMed

    Kato, Masao; Fujiura, Kazuo; Kurihara, Takashi

    2005-03-10

    Ultrafast asynchronous all-optical signal processing is experimentally demonstrated. It is based on the intensity-dependent, self-frequency shift of a gigahertz Raman soliton. We demonstrate error-free, asynchronous, all-optical, bit-by-bit, self-signal recognition and demultiplexing from contended optical packets without use of an optical buffer, control pulse, or bit-phase synchronization. Fourfold, contended, 9.95-Gbit/s optical packets are transmitted through a conventional repeater span of 80 km and simultaneously demultiplexed to multiwavelength 9.95-Gbit/s optical packets with 0.5-dB processing sensitivity. Furthermore, we successfully accomplish demultiplexing from overlapping signals in contended optical packets with better than 3-dB recognition sensitivity. We confirm the capability of realizing a 3x cascade operation from bit-error-rate measurements.

  13. Research on the enhancement of signal-to-noise ratio of light-addressable potentiometric sensor by optical focusing

    NASA Astrophysics Data System (ADS)

    Chen, Dong; Liu, Shi-bin; Yin, Shi-min; Liang, Jin-tao

    2016-01-01

    For enhancing the response of light-addressable potentiometric sensor (LAPS) and further improving its signal- to-noise ratio ( SNR), an optical focusing method is adopted. Experimental research and theoretical analysis reveal that the magnitude of responsive signal is increased by optical focusing, and the SNR is improved remarkably. These research results indicate that the optical focusing is an effective approach for improving SNR of LAPS.

  14. Characterizing relationship between optical microangiography signals and capillary flow using microfluidic channels.

    PubMed

    Choi, Woo June; Qin, Wan; Chen, Chieh-Li; Wang, Jingang; Zhang, Qinqin; Yang, Xiaoqi; Gao, Bruce Z; Wang, Ruikang K

    2016-07-01

    Optical microangiography (OMAG) is a powerful optical angio-graphic tool to visualize micro-vascular flow in vivo. Despite numerous demonstrations for the past several years of the qualitative relationship between OMAG and flow, no convincing quantitative relationship has been proven. In this paper, we attempt to quantitatively correlate the OMAG signal with flow. Specifically, we develop a simplified analytical model of the complex OMAG, suggesting that the OMAG signal is a product of the number of particles in an imaging voxel and the decorrelation of OCT (optical coherence tomography) signal, determined by flow velocity, inter-frame time interval, and wavelength of the light source. Numerical simulation with the proposed model reveals that if the OCT amplitudes are correlated, the OMAG signal is related to a total number of particles across the imaging voxel cross-section per unit time (flux); otherwise it would be saturated but its strength is proportional to the number of particles in the imaging voxel (concentration). The relationship is validated using microfluidic flow phantoms with various preset flow metrics. This work suggests OMAG is a promising quantitative tool for the assessment of vascular flow. PMID:27446700

  15. Research on sensor technology of Lamb-wave signal acquisition using optical low-coherence

    NASA Astrophysics Data System (ADS)

    Zhu, Y. K.; Yang, C.; Li, X. W.; Chong, B.

    2012-10-01

    Non-destructive testing of composite materials is a key technology issue in equipment testing. Among the emerging new testing methods, Lamb-wave technology is getting more and more attention. This paper proposed a sensing method to acquire the Lamb-wave signal in thin plate based on optical low-coherence principles. Methods to acquire Lamb-wave in thin plate using optical low-coherence technology were analyzed, and the technical path of non-contact, high-precision method was chosen. Complete in-line experimental system and methods were designed and built up for testing. A sensor system based on Michelson low-coherence interferometer was set up. The distributed optical fiber sensors were arranged on the top of sample materials for signal detection. Mirrors to enhance reflection intensity were attached on the sample. The phase of sensing arm was modulated by PZT vibration. Then signals were detected and processed by Daubechies10 wavelet and Gabor wavelet. In-line testing of thin plate with features of high-precision and high signal-noise-ratio was realized, which is meaningful to dynamic testing of large-scale structure.

  16. Characterizing relationship between optical microangiography signals and capillary flow using microfluidic channels

    PubMed Central

    Choi, Woo June; Qin, Wan; Chen, Chieh-Li; Wang, Jingang; Zhang, Qinqin; Yang, Xiaoqi; Gao, Bruce Z.; Wang, Ruikang K.

    2016-01-01

    Optical microangiography (OMAG) is a powerful optical angio-graphic tool to visualize micro-vascular flow in vivo. Despite numerous demonstrations for the past several years of the qualitative relationship between OMAG and flow, no convincing quantitative relationship has been proven. In this paper, we attempt to quantitatively correlate the OMAG signal with flow. Specifically, we develop a simplified analytical model of the complex OMAG, suggesting that the OMAG signal is a product of the number of particles in an imaging voxel and the decorrelation of OCT (optical coherence tomography) signal, determined by flow velocity, inter-frame time interval, and wavelength of the light source. Numerical simulation with the proposed model reveals that if the OCT amplitudes are correlated, the OMAG signal is related to a total number of particles across the imaging voxel cross-section per unit time (flux); otherwise it would be saturated but its strength is proportional to the number of particles in the imaging voxel (concentration). The relationship is validated using microfluidic flow phantoms with various preset flow metrics. This work suggests OMAG is a promising quantitative tool for the assessment of vascular flow. PMID:27446700

  17. Optical phase-locked loop signal sources for phased-array communications antennas

    NASA Astrophysics Data System (ADS)

    Langley, Lloyd N.; Edge, Colin; Wale, Michael J.; Gliese, Ulrik B.; Seeds, Alwyn J.; Walton, Channing; Wright, James G.; Coryell, Louis A.

    1997-10-01

    A coherent, optical heterodyne approach to signal generation and beamforming is particularly advantageous in multi-beam mobile phased arrays. Use of optical technology allows an optimum distribution of weight and power to be achieved between the antenna face and central electronics, together with an efficient implementation of the beamforming function and a modular design approach in which the basic building blocks are frequency-independent. Systems of this type employ a pair of optical carriers with a difference frequency equal to the required microwave signal. Phased- locking is necessary in order to achieve sufficiently low phase noise in the radio communication link. Optical phase locked loops (OPLLs) have been shown to be potential candidates for this application, yet work still needs to be done to bring them from the laboratory to field demonstrations. This paper describes the construction of a laser-diode OPLL subsystem for evaluation in a proof-of- concept beamforming system. This involves optimization of the loop design, development of single-frequency laser diodes with the correct linewidth, modulation and tuning characteristics and integration into a micro-optic assembly with custom wideband electronics.

  18. Network coding based joint signaling and dynamic bandwidth allocation scheme for inter optical network unit communication in passive optical networks

    NASA Astrophysics Data System (ADS)

    Wei, Pei; Gu, Rentao; Ji, Yuefeng

    2014-06-01

    As an innovative and promising technology, network coding has been introduced to passive optical networks (PON) in recent years to support inter optical network unit (ONU) communication, yet the signaling process and dynamic bandwidth allocation (DBA) in PON with network coding (NC-PON) still need further study. Thus, we propose a joint signaling and DBA scheme for efficiently supporting differentiated services of inter ONU communication in NC-PON. In the proposed joint scheme, the signaling process lays the foundation to fulfill network coding in PON, and it can not only avoid the potential threat to downstream security in previous schemes but also be suitable for the proposed hybrid dynamic bandwidth allocation (HDBA) scheme. In HDBA, a DBA cycle is divided into two sub-cycles for applying different coding, scheduling and bandwidth allocation strategies to differentiated classes of services. Besides, as network traffic load varies, the entire upstream transmission window for all REPORT messages slides accordingly, leaving the transmission time of one or two sub-cycles to overlap with the bandwidth allocation calculation time at the optical line terminal (the OLT), so that the upstream idle time can be efficiently eliminated. Performance evaluation results validate that compared with the existing two DBA algorithms deployed in NC-PON, HDBA demonstrates the best quality of service (QoS) support in terms of delay for all classes of services, especially guarantees the end-to-end delay bound of high class services. Specifically, HDBA can eliminate queuing delay and scheduling delay of high class services, reduce those of lower class services by at least 20%, and reduce the average end-to-end delay of all services over 50%. Moreover, HDBA also achieves the maximum delay fairness between coded and uncoded lower class services, and medium delay fairness for high class services.

  19. Semiconductor optical amplifier-based heterodyning detection for resolving optical terahertz beat-tone signals from passively mode-locked semiconductor lasers

    SciTech Connect

    Latkowski, Sylwester; Maldonado-Basilio, Ramon; Carney, Kevin; Parra-Cetina, Josue; Philippe, Severine; Landais, Pascal

    2010-08-23

    An all-optical heterodyne approach based on a room-temperature controlled semiconductor optical amplifier (SOA) for measuring the frequency and linewidth of the terahertz beat-tone signal from a passively mode-locked laser is proposed. Under the injection of two external cavity lasers, the SOA acts as a local oscillator at their detuning frequency and also as an optical frequency mixer whose inputs are the self-modulated spectrum of the device under test and the two laser beams. Frequency and linewidth of the intermediate frequency signal (and therefore, the beat-tone signal) are resolved by using a photodiode and an electrical spectrum analyzer.

  20. Generation and distribution of a wide-band continuously tunable millimeter-wave signal with an optical external modulation technique

    NASA Astrophysics Data System (ADS)

    Qi, Guohua; Yao, Jianping; Seregelyi, J.; Paquet, S.; Belisle, C.

    2005-10-01

    A new technique to generate and distribute a wide-band continuously tunable millimeter-wave signal using an optical external modulator and a wavelength-fixed optical notch filter is proposed. The optical intensity modulator is biased to suppress the odd-order optical sidebands. The wavelength-fixed optical notch filter is then used to filter out the optical carrier. Two second-order optical sidebands are obtained at the output of the notch filter. A millimeter-wave signal that has four times the frequency of the microwave drive signal is generated by beating the two second-order optical sidebands at a photodetector. Since no tunable optical filter is used, the system is easy to implement. A system using an LiNbO3 intensity modulator and a fiber Bragg grating filter is built. A stable and high spectral purity millimeter-wave signal tunable from 32 to 50 GHz is obtained by tuning the microwave drive signal from 8 to 12.5 GHz. The integrity of the generated millimeter-wave signal is maintained after transmission over a 25-km standard single-mode fiber. Theoretical analysis on the harmonic suppression with different modulation depths and filter attenuations is also discussed.

  1. All-optical demultiplexing of 16-QAM signals into QPSK tributaries using four-level optical phase quantizers.

    PubMed

    Bogris, Adonis

    2014-04-01

    The potential of four-level optical phase quantizers toward coherent processing of advanced modulation formats, such as 16-QAM, is proposed and numerically demonstrated. The work shows that phase quantization achieved in fiber-based phase-sensitive amplifiers can demultiplex 16-QAM into two quadrature phase shift keying (QPSK) signals, enabling subchannel switching. The numerical study highlights the impact of the quantizer transfer function on the performance of the demultiplexing process and numerically calculates the bit error rate for each QPSK tributary after the demultiplexing procedure.

  2. Combining signals of widely differing bit rates on fiber optic subscriber lines

    NASA Astrophysics Data System (ADS)

    Hall, Michael

    The publication is based on research on optical broad-band transmission in the subscriber loop. The first part of the work is devoted to obtaining a general review on the different methods of combining digital signals of widely differing bit rates. The review covers various time division multiplexing formats, wavelength and frequency division multiplexing, and methods where the high bit rate signal is modulated by the low bit rate signal. Two multiplexing methods, frequency division multiplexing and code division multiplexing, are analyzed in greater detail. It is shown that code division multiplexing in conjunction with a convenient sampling method for the low bit rate signal results in a simple, flexible and all digital transmission system.

  3. Long-range vibration sensor based on correlation analysis of optical frequency-domain reflectometry signals.

    PubMed

    Ding, Zhenyang; Yao, X Steve; Liu, Tiegen; Du, Yang; Liu, Kun; Han, Qun; Meng, Zhuo; Chen, Hongxin

    2012-12-17

    We present a novel method to achieve a space-resolved long- range vibration detection system based on the correlation analysis of the optical frequency-domain reflectometry (OFDR) signals. By performing two separate measurements of the vibrated and non-vibrated states on a test fiber, the vibration frequency and position of a vibration event can be obtained by analyzing the cross-correlation between beat signals of the vibrated and non-vibrated states in a spatial domain, where the beat signals are generated from interferences between local Rayleigh backscattering signals of the test fiber and local light oscillator. Using the proposed technique, we constructed a standard single-mode fiber based vibration sensor that can have a dynamic range of 12 km and a measurable vibration frequency up to 2 kHz with a spatial resolution of 5 m. Moreover, preliminarily investigation results of two vibration events located at different positions along the test fiber are also reported.

  4. Polarization insensitive all-optical wavelength conversion of polarization multiplexed signals using co-polarized pumps.

    PubMed

    Anthur, Aravind P; Zhou, Rui; O'Duill, Sean; Walsh, Anthony J; Martin, Eamonn; Venkitesh, Deepa; Barry, Liam P

    2016-05-30

    We study and experimentally validate the vector theory of four-wave mixing (FWM) in semiconductor optical amplifiers (SOA). We use the vector theory of FWM to design a polarization insensitive all-optical wavelength converter, suitable for advanced modulation formats, using non-degenerate FWM in SOAs and parallelly polarized pumps. We demonstrate the wavelength conversion of polarization-multiplexed (PM)-QPSK, PM-16QAM and a Nyquist WDM super-channel modulated with PM-QPSK signals at a baud rate of 12.5 GBaud, with total data rates of 50 Gbps, 100 Gbps and 200 Gbps respectively. PMID:27410100

  5. Determination of the axial stiffness of an optical trap with information entropy signals

    NASA Astrophysics Data System (ADS)

    Zhong, Mincheng; Zhou, Jinhua; Wu, Jianguang; Li, Yinmei

    2009-11-01

    Optical tweezers has been used to manipulate micro-sized particles for many years, and has been widely used in various applications. The axial trapping stiffness is one of the most important parameters to evaluate the trapping ability of an optical tweezers. In this paper, we calibrated the axial optical stiffnesses for micro-sized polystyrene spheres. When an external force was applied to particle held by an optical trap, the particle was displaced from the trap center by an amount proportional to the applied force. We displaced the particle from the trap center by applying triangular waves of varying velocity, and the varying velocity was obtained by altering the frequency of the triangular waves. In this case the particle has two balance position distributed at two-side of the trap center. The calibration of the axial position was critical to the measurement of axial optical stiffness. In this paper, the axial displacement between the balance position and the trap center was calibrated with image information entropy signals. According to Stokes Law, when the axial displacement of the particle relative to the external force was known, the axial optical stiffness can be measured, and this method was known as viscous drag method. The stiffnesses for a 2μm-diameter at different trapped depth were measured. Typical values for axial optical stiffness of our optical tweezers were between 4.0 and 7.5 pN/μm when the laser power was 35mW. Dependence of axial optical trapping stiffness on the diameter of the particles was measured with viscous drag method. At last, the origin of the measurement error was discussed.

  6. Quantitative evaluation of optical coherence tomography signal enhancement with gold nanoshells.

    PubMed

    Agrawal, Anant; Huang, Stanley; Wei Haw Lin, Alex; Lee, Min-Ho; Barton, Jennifer K; Drezek, Rebekah A; Pfefer, T Joshua

    2006-01-01

    Nanoshell-enhanced optical coherence tomography (OCT) is a novel technique with the potential for molecular imaging and improved disease detection. However, optimization of this approach will require a quantitative understanding of the influence of nanoshell parameters on detected OCT signals. In this study, OCT was performed at 1310 nm in water and turbid tissue-simulating phantoms to which nanoshells were added. The effect of nanoshell concentration, core diameter, and shell thickness on signal enhancement was characterized. Experimental results indicated trends that were consistent with predicted optical properties-a monotonic increase in signal intensity and attenuation with increasing shell and core size. Threshold concentrations for a 2-dB OCT signal intensity gain were determined for several nanoshell geometries. For the most highly backscattering nanoshells tested-291-nm core diameter, 25-nm shell thickness-a concentration of 10(9) nanoshells/mL was needed to produce this signal increase. Based on these results, we discuss various practical considerations for optimizing nanoshell-enhanced OCT. Quantitative experimental data presented here will facilitate optimization of OCT-based diagnostics and may also be relevant to other reflectance-based approaches as well. PMID:16965149

  7. The nanosecond optical parametric amplifier of a weak signal based on BBO crystals

    NASA Astrophysics Data System (ADS)

    Bagdasarov, V. Kh; Bel'kov, S. A.; Garanin, S. G.; Garnov, S. V.; Nikolaev, D. A.; Orlov, S. N.; Polivanov, Y. N.; Shcherbakov, I. A.; Tsvetkov, V. B.

    2016-09-01

    Parameters of the optical parametric amplifier (OPA), based on two BBO crystals were studied. The OPA was made with the schematic of the extraordinary wave walk off compensation. Efficient amplification of the weak signal (λ  =  1053 nm) in the field of the strong pumping wave (λ  =  532 nm) was obtained. The measured value of the amplification was equal to ~106. The noise level of the parametric amplifier was less than 10-3 from the signal level.

  8. Flexible generation of optical nonuniform bit-mapping signal based on InP transmitter module.

    PubMed

    Liu, Bo; Zhang, Lijia; Xin, Xiangjun; Liu, Lei

    2014-01-15

    Flexible generation of an optical nonuniform bit-mapping signal based on an InP transmitter module is demonstrated. It can realize flexible bit mapping through the photonic modulation method, which can break the limitations of an electrical digital-to-analog convertor and field-programmable gate array. This module has potential to easily increase the signal rate or refine the granularity without electronics, which indicates it may be a future application to replace the traditional transmitter. The feasibility and performance of the proposed scheme are demonstrated in the experiment.

  9. Suppression of nonlinear optical signals in finite interaction volumes of bulk materials.

    PubMed

    Cattaneo, Stefano; Siltanen, Mikael; Xiang Wang, Fu; Kauranen, Martti

    2005-11-28

    We show that nonlinear optical signals generated by non-phase-matched interactions are strongly suppressed when the interaction volume is finite and localized deep inside the bulk of a homogeneous material, as opposed to the case where the interaction volume extends across a boundary of the material. The suppression in the bulk originates from destructive interference between the signals generated in the two regions where the interaction is gradually turned on and off and depends on the ratio of the coherence length to the characteristic length of the interaction volume.

  10. The nanosecond optical parametric amplifier of a weak signal based on BBO crystals

    NASA Astrophysics Data System (ADS)

    Bagdasarov, V. Kh; Bel’kov, S. A.; Garanin, S. G.; Garnov, S. V.; Nikolaev, D. A.; Orlov, S. N.; Polivanov, Y. N.; Shcherbakov, I. A.; Tsvetkov, V. B.

    2016-09-01

    Parameters of the optical parametric amplifier (OPA), based on two BBO crystals were studied. The OPA was made with the schematic of the extraordinary wave walk off compensation. Efficient amplification of the weak signal (λ  =  1053 nm) in the field of the strong pumping wave (λ  =  532 nm) was obtained. The measured value of the amplification was equal to ~106. The noise level of the parametric amplifier was less than 10‑3 from the signal level.

  11. Tunable microwave signal generator with an optically-injected 1310 nm QD-DFB laser.

    PubMed

    Hurtado, Antonio; Mee, Jesse; Nami, Mohsen; Henning, Ian D; Adams, Michael J; Lester, Luke F

    2013-05-01

    Tunable microwave signal generation with frequencies ranging from below 1 GHz to values over 40 GHz is demonstrated experimentally with a 1310 nm Quantum Dot (QD) Distributed-Feedback (DFB) laser. Microwave signal generation is achieved using the period 1 dynamics induced in the QD DFB under optical injection. Continuous tuning in the positive detuning frequency range of the quantum dot's unique stability map is demonstrated. The simplicity of the experimental configuration offers promise for novel uses of these nanostructure lasers in Radio-over-Fiber (RoF) applications and future mobile networks.

  12. Amplification of optical signals in Bi{sub 12}TiO{sub 20} crystal by photorefractive surface waves

    SciTech Connect

    Khomenko, A.V.; Garcia-Weidner, A.; Kamshilin, A.A.

    1996-07-01

    We have demonstrated experimentally beam amplification by coupling between the signal beam and the photorefractive surfaces wave in Bi{sub 12}TiO{sub 20} crystal. A gain of 16,000 has been measured, with an output signal-to-noise ratio of {approx_gt}20 for weak input signals. {copyright} {ital 1996 Optical Society of America.}

  13. Bioinspired Polarization Imaging Sensors: From Circuits and Optics to Signal Processing Algorithms and Biomedical Applications

    PubMed Central

    York, Timothy; Powell, Samuel B.; Gao, Shengkui; Kahan, Lindsey; Charanya, Tauseef; Saha, Debajit; Roberts, Nicholas W.; Cronin, Thomas W.; Marshall, Justin; Achilefu, Samuel; Lake, Spencer P.; Raman, Baranidharan; Gruev, Viktor

    2015-01-01

    In this paper, we present recent work on bioinspired polarization imaging sensors and their applications in biomedicine. In particular, we focus on three different aspects of these sensors. First, we describe the electro–optical challenges in realizing a bioinspired polarization imager, and in particular, we provide a detailed description of a recent low-power complementary metal–oxide–semiconductor (CMOS) polarization imager. Second, we focus on signal processing algorithms tailored for this new class of bioinspired polarization imaging sensors, such as calibration and interpolation. Third, the emergence of these sensors has enabled rapid progress in characterizing polarization signals and environmental parameters in nature, as well as several biomedical areas, such as label-free optical neural recording, dynamic tissue strength analysis, and early diagnosis of flat cancerous lesions in a murine colorectal tumor model. We highlight results obtained from these three areas and discuss future applications for these sensors. PMID:26538682

  14. [Improving the signal to noise ratio of spectroscopy for tongue inspection by optical modulation].

    PubMed

    Li, Gang; Zhao, Jing; Lin, Ling; Wu, Xiao-rong

    2012-01-01

    In order to solve the problem that the collected spectrum was influenced seriously by the environment light, optical modulation was used to improve the signal to noise ratio of spectroscopy-tongue inspection for the first time. First, reference light was modulated by reticle in this system; second, the reflect spectrum of the same measured object was collected under the condition that it was influenced by green, purple, red laser and environment light respectively; thirdly, the DFT was used to demodulate the collected spectrum data; the maximum harmonic components were extracted and normalized, then the spectral curve was drawn; by comparison, the spectral curves of the same measured object under different condition were almost the same. The experiment result showed that the optical modulation can remove the influence of the environment light; and this method can provide a new way to improve the signal to noise ratio of spectroscopy for tongue inspection. PMID:22497157

  15. Optical wavefront shaping for the enhancement of Raman signal in scattering media

    NASA Astrophysics Data System (ADS)

    Thompson, Jonathan V.; Throckmorton, Graham A.; Hokr, Brett H.; Yakovlev, Vladislav V.

    2016-03-01

    The ability to non-invasively focus light through scattering media has significant applications in many fields ranging from nanotechnology to deep tissue sensing. Until recently, the multiple light scattering events that occur in complex media such as biological tissue have inhibited the focusing ability and penetration depth of optical tools. Through the use of optical wavefront shaping, the spatial distortions due to these scattering events can be corrected, and the incident light can be focused through the scattering medium. Here, we demonstrate that wavefront shaping can be used to non-invasively enhance the Raman signal of a material through a scattering medium. Raman signal enhancement was achieved using backscattered light and a continuous sequential algorithm. Our results show the potential of wavefront shaping as an important addition to non-invasive detection techniques.

  16. Signal of microstrip scanning near-field optical microscope in far- and near-field zones.

    PubMed

    Morozov, Yevhenii M; Lapchuk, Anatoliy S

    2016-05-01

    An analytical model of interference between an electromagnetic field of fundamental quasi-TM(EH)00-mode and an electromagnetic field of background radiation at the apex of a near-field probe based on an optical plasmon microstrip line (microstrip probe) has been proposed. The condition of the occurrence of electromagnetic energy reverse flux at the apex of the microstrip probe was obtained. It has been shown that the nature of the interference depends on the length of the probe. Numerical simulation of the sample scanning process was conducted in illumination-reflection and illumination-collection modes. Results of numerical simulation have shown that interference affects the scanning signal in both modes. However, in illumination-collection mode (pure near-field mode), the signal shape and its polarity are practically insensible to probe length change; only signal amplitude (contrast) is slightly changed. However, changing the probe length strongly affects the signal amplitude and shape in the illumination-reflection mode (the signal formed in the far-field zone). Thus, we can conclude that even small background radiation can significantly influence the signal in the far-field zone and has practically no influence on a pure near-field signal. PMID:27140358

  17. Full-duplex radio over fiber link with colorless source-free base station based on single sideband optical mm-wave signal with polarization rotated optical carrier

    NASA Astrophysics Data System (ADS)

    Ma, Jianxin

    2016-07-01

    A full-duplex radio-over fiber (RoF) link scheme based on single sideband (SSB) optical millimeter (mm)-wave signal with polarization-rotated optical carrier is proposed to realize the source-free colorless base station (BS), in which a polarization beam splitter (PBS) is used to abstract part of the optical carrier for conveying the uplink data. Since the optical carrier for the uplink does not bear the downlink signal, no cross-talk from the downlink contaminates the uplink signal. The simulation results demonstrate that both down- and up-links maintain good performance. The mm-wave signal distribution network based on the proposed full duplex fiber link scheme can use the uniform source-free colorless BSs, which makes the access system very simpler.

  18. Plastic fiber optics for micro-imaging of fluorescence signals in living cells

    NASA Astrophysics Data System (ADS)

    Sakurai, Takashi; Natsume, Mitsuo; Koida, Kowa

    2015-03-01

    The fiber-coupled microscope (FCM) enables in vivo imaging at deep sites in the tissues or organs that other optical techniques are unable to reach. To develop FCM-based intravital imaging, we employed a plastic optical fiber (POF) bundle that included more than 10,000-units of polystyrene core and polymethyl methacrylate cladding. Each POF had a diameter of less than 5 μm the tip of the bundle was less than 0.5 mm wide, and the flexible wire had a length of 1,000 mm. The optical performance of the plastic FCM was sufficient for detection of significant signal changes in an acinus of rat pancreas labeled with a calcium ion-sensitive fluorescent dye. In the future, the potential power of plastic FCM is expected to increase, enabling analysis of structure and organization of specific functions in live cells within vulnerable organs.

  19. Realization of rapid debugging for detection circuit of optical fiber gas sensor: Using an analog signal source

    NASA Astrophysics Data System (ADS)

    Tian, Changbin; Chang, Jun; Wang, Qiang; Wei, Wei; Zhu, Cunguang

    2015-03-01

    An optical fiber gas sensor mainly consists of two parts: optical part and detection circuit. In the debugging for the detection circuit, the optical part usually serves as a signal source. However, in the debugging condition, the optical part can be easily influenced by many factors, such as the fluctuation of ambient temperature or driving current resulting in instability of the wavelength and intensity for the laser; for dual-beam sensor, the different bends and stresses of the optical fiber will lead to the fluctuation of the intensity and phase; the intensity noise from the collimator, coupler, and other optical devices in the system will also result in the impurity of the optical part based signal source. In order to dramatically improve the debugging efficiency of the detection circuit and shorten the period of research and development, this paper describes an analog signal source, consisting of a single chip microcomputer (SCM), an amplifier circuit, and a voltage-to-current conversion circuit. It can be used to realize the rapid debugging detection circuit of the optical fiber gas sensor instead of optical part based signal source. This analog signal source performs well with many other advantages, such as the simple operation, small size, and light weight.

  20. Optical multi-coset sampling of GHz-band chirped signals

    NASA Astrophysics Data System (ADS)

    Valley, George C.; Sefler, George A.; Shaw, T. J.; Smith, Stephen L.

    2015-03-01

    Direct digitization of long, wideband chirped RF signals in the GHz band requires power hungry ADCs and produces large data sets. Here we present an optical scheme to perform multi-coset sampling of such signals with reduced power consumption and smaller data sets. In our scheme a repetitively pulsed femtosecond laser is dispersed to the interpulse time, the RF is modulated on the optical field, and the field is directed to a pair of wavelength-division demultiplexers (WDM). The channels of the WDM are attenuated with a pseudo-random sequence to form a coset pattern that repeats at the laser repetition rate. After a photodiode, the photocurrent is integrated for the duration of the dispersed optical pulse so that the coset pattern non-uniformly samples the RF signal. Since the laser repetition rate is uncorrelated with the RF, each coset provides an independent measurement of the RF. Experimental and numerical results show that 4 properties of the RF chirp pulse can be determined from the multiple coset samples: carrier frequency, chirp rate, start time, and pulse duration. Results are presented for a 20MHz chirp on a 13 microsecond pulse at a carrier of 2.473 GHz.

  1. Delay Tracking of Spread-Spectrum Signals for Indoor Optical Ranging

    PubMed Central

    Salido-Monzú, David; Martín-Gorostiza, Ernesto; Lázaro-Galilea, José Luis; Martos-Naya, Eduardo; Wieser, Andreas

    2014-01-01

    Delay tracking of spread-spectrum signals is widely used for ranging in radio frequency based navigation. Its use in non-coherent optical ranging, however, has not been extensively studied since optical channels are less subject to narrowband interference situations where these techniques become more useful. In this work, an early-late delay-locked loop adapted to indoor optical ranging is presented and analyzed. The specific constraints of free-space infrared channels in this context substantially differ from those typically considered in radio frequency applications. The tracking stage is part of an infrared differential range measuring system with application to mobile target indoor localization. Spread-spectrum signals are used in this context to provide accurate ranging while reducing the effect of multipath interferences. The performance of the stage regarding noise and dynamic errors is analyzed and validated, providing expressions that allow an adequate selection of the design parameters depending on the expected input signal characteristics. The behavior of the stage in a general multipath scenario is also addressed to estimate the multipath error bounds. The results, evaluated under realistic conditions corresponding to an 870 nm link with 25 MHz chip-rate, built with low-cost up-to-date devices, show that an overall error below 6% of a chip time can be achieved. PMID:25490585

  2. Delay tracking of spread-spectrum signals for indoor optical ranging.

    PubMed

    Salido-Monzú, David; Martín-Gorostiza, Ernesto; Lázaro-Galilea, José Luis; Martos-Naya, Eduardo; Wieser, Andreas

    2014-01-01

    Delay tracking of spread-spectrum signals is widely used for ranging in radio frequency based navigation. Its use in non-coherent optical ranging, however, has not been extensively studied since optical channels are less subject to narrowband interference situations where these techniques become more useful. In this work, an early-late delay-locked loop adapted to indoor optical ranging is presented and analyzed. The specific constraints of free-space infrared channels in this context substantially differ from those typically considered in radio frequency applications. The tracking stage is part of an infrared differential range measuring system with application to mobile target indoor localization. Spread-spectrum signals are used in this context to provide accurate ranging while reducing the effect of multipath interferences. The performance of the stage regarding noise and dynamic errors is analyzed and validated, providing expressions that allow an adequate selection of the design parameters depending on the expected input signal characteristics. The behavior of the stage in a general multipath scenario is also addressed to estimate the multipath error bounds. The results, evaluated under realistic conditions corresponding to an 870 nm link with 25 MHz chip-rate, built with low-cost up-to-date devices, show that an overall error below 6% of a chip time can be achieved.

  3. Wideband Analog Fiber Optic Signal Link For Use In The Space/Radiation Simulator Environment

    NASA Astrophysics Data System (ADS)

    Blackburn, J.; Vanderwall, J.; Gilbert, R.; Holliday, V.

    1982-01-01

    The report describes a wideband linear response fiber optic transmission link designed to telemeter signals from a system being irradiated in a space/radiation simulator. The environment is severe: the transmitter must withstand an x-ray pulse delivering 109 rad(Si)/sec (surface dose rate), beams of 300 - 1000 KeV electrons, hard vacuum, and liquid nitrogen temperatures without degradation or even momentary upset. Radiation hardness is achieved by a combination of circuit design and high-Z shielding, including an optical fiber shield made of non-conducting lead-loaded. polyethylene. Special heat. conducting and insulating measures are employed to maintain temperature. The transmitter measures 15 x 8.5 x 9.5 am and operates on self-contained batteries. Signal bandwidth is greater than 10 KHz to 400 MHz with approximately 35 dB, of dynamic range. Signal inputs ranging from one millivolt to several volts are accommodated; four single-or double-ended inputs are provided, with remote selection. The single-fiber coupled remote control also allows adjusting input. attenuation, power and calibrator control, and verification of transmitter function. The single-mode laser used as the high frequency optical source is microwave dither-blased1 to reduce modal noise.

  4. Is Anybody Out There? The Search for Radio and Optical Signals from Extraterrestrial Civilizations

    SciTech Connect

    Werthimer, Daniel

    2003-04-23

    Werthimer will discuss the search for radio and laser signals from other civilizations and review the SETI{at}home, SERENDIP and Astropulse radio SETI programs as well as the SEVENDIP and SPOCK optical SETI Programs. The SERENDIP SETI sky survey searches for narrow band radio signals at the 305 meter Arecibo Observatory in Puerto Rico. SETI@home uses four million volunteers to analyse 50 Terabytes of data from Arecibo. SETI{at}home is the largest supercomputer on the planet, currently averaging 40 Teraflops. SETI{at}home participants have donated one million CPU years of computer time. The upcoming Astropulse search will use distributed computing to search for uS time scale radio pulses from evaporating black holes, pulsars or ET. The SEVENDIP optical SETI program searches for nS timescale pulses at visible wavelengths. The Spock optical SETI program searches for narrow band coherent signals in high resolution stellar spectra taken at Keck observatory. More info at http://seti.berkeley.edu

  5. Method of optical coherence tomography with parallel depth-resolved signal reception and fibre-optic phase modulators

    SciTech Connect

    Morozov, A N; Turchin, I V

    2013-12-31

    The method of optical coherence tomography with the scheme of parallel reception of the interference signal (P-OCT) is developed on the basis of spatial paralleling of the reference wave by means of a phase diffraction grating producing the appropriate time delay in the Mach–Zehnder interferometer. The absence of mechanical variation of the optical path difference in the interferometer essentially reduces the time required for 2D imaging of the object internal structure, as compared to the classical OCT that uses the time-domain method of the image construction, the sensitivity and the dynamic range being comparable in both approaches. For the resulting field of the interfering object and reference waves an analytical expression is derived that allows the calculation of the autocorrelation function in the plane of photodetectors. For the first time a method of linear phase modulation by 2π is proposed for P-OCT systems, which allows the use of compact high-frequency (a few hundred kHz) piezoelectric cell-based modulators. For the demonstration of the P-OCT method an experimental setup was created, using which the images of the inner structure of biological objects at the depth up to 1 mm with the axial spatial resolution of 12 μm were obtained. (optical coherence tomography)

  6. Optical observations of meteors generating infrasound-I: Acoustic signal identification and phenomenology

    NASA Astrophysics Data System (ADS)

    Silber, Elizabeth A.; Brown, Peter G.

    2014-11-01

    We analyse infrasound signals from 71 bright meteors/fireballs simultaneously detected by video to investigate the phenomenology and characteristics of meteor-generated near-field infrasound (<300 km) and shock production. A taxonomy for meteor generated infrasound signal classification has been developed using the time-pressure signal of the infrasound arrivals. Based on the location along the meteor trail where the infrasound signal originates, we find most signals are associated with cylindrical shocks, with about a quarter of events evidencing spherical shocks associated with fragmentation episodes and optical flares. The video data indicate that all events with ray launch angles >117° from the trajectory heading are most likely generated by a spherical shock, while infrasound produced by the meteors with ray launch angles ≤117° can be attributed to both a cylindrical line source and a spherical shock. We find that meteors preferentially produce infrasound toward the end of their trails with a smaller number showing a preference for mid-trail production. Meteors producing multiple infrasound arrivals show a strong infrasound source height skewness to the end of trails and are much more likely to be associated with optical flares. We find that about 1% of all our optically-recorded meteors have associated detected infrasound and estimate that regional meteor infrasound events should occur on the order of once per week and dominate in numbers over infrasound associated with more energetic (but rarer) bolides. While a significant fraction of our meteors generating infrasound (~1/4 of single arrivals) are produced by fragmentation events, we find no instances where acoustic radiation is detectable more than about 60° beyond the ballistic regime at our meteoroid sizes (grams to tens of kilograms) emphasizing the strong anisotropy in acoustic radiation for meteors which are dominated by cylindrical line source geometry, even in the presence of fragmentation.

  7. Reversible and irreversible emergence of chiroptical signals in J-aggregates of achiral 4-sulfonatophenyl substituted porphyrins: intrinsic chirality vs. chiral ordering in the solution.

    PubMed

    Arteaga, Oriol; El-Hachemi, Zoubir; Canillas, Adolf; Crusats, Joaquim; Rovira, Meritxell; Ribó, Josep M

    2016-09-18

    Mueller matrix polarimetry distinguishes the different origins of the reversible and irreversible chiroptical effects emerging in stirred solutions of J-aggregate nanoparticles: the reversible effect is due to an anisotropic ordering in the solution and the irreversible one is due to a bias from the racemic composition of intrinsically chiral structures.

  8. Adaptive Integrated Optical Bragg Grating in Semiconductor Waveguide Suitable for Optical Signal Processing

    NASA Astrophysics Data System (ADS)

    Moniem, T. A.

    2016-05-01

    This article presents a methodology for an integrated Bragg grating using an alloy of GaAs, AlGaAs, and InGaAs with a controllable refractive index to obtain an adaptive Bragg grating suitable for many applications on optical processing and adaptive control systems, such as limitation and filtering. The refractive index of a Bragg grating is controlled by using an external electric field for controlling periodic modulation of the refractive index of the active waveguide region. The designed Bragg grating has refractive indices programmed by using that external electric field. This article presents two approaches for designing the controllable refractive indices active region of a Bragg grating. The first approach is based on the modification of a planar micro-strip structure of the iGaAs traveling wave as the active region, and the second is based on the modification of self-assembled InAs/GaAs quantum dots of an alloy from GaAs and InGaAs with a GaP traveling wave. The overall design and results are discussed through numerical simulation by using the finite-difference time-domain, plane wave expansion, and opto-wave simulation methods to confirm its operation and feasibility.

  9. The sequence of events in a lightning stroke: terrestrial gamma ray flash, radio signal and optical lightning.

    NASA Astrophysics Data System (ADS)

    Ostgaard, N.; Gjesteland, T.; Christian, H. J., Jr.; Albrechtsen, K.; Carlson, B. E.; Collier, A.; Cummer, S.; Lu, G.

    2015-12-01

    Two observations of simultaneous terrestrial gamma ray flashes (RHESSI) and optical lightning (LIS) from space and radio signals on ground (WWLLN and Duke) will be presented. Both events indicate that radio waves come from the TGF itself, while the optical signal is slightly delayed. These observations are consistent with the TGF being produced in one of the last leader steps in an IC+ lightning stroke, and that the optical signal is produced when the leader reaches the positive charge layer.We will also present results showing that there are even more weak TGFs, than currently reported, in the RHESSI data set.

  10. Alternative theory of diffraction grating spectral device and its application for calculation of convolution and correlation of optical pulse signals

    NASA Astrophysics Data System (ADS)

    Kazakov, Vasily I.; Moskaletz, Dmitry O.; Moskaletz, Oleg D.

    2016-04-01

    A new, alternative theory of diffraction grating spectral device which is based on the mathematical analysis of the optical signal transformation from the input aperture of spectral device to result of photo detection is proposed. Exhaustive characteristics of the diffraction grating spectral device - its complex and power spread functions as the kernels of the corresponding integral operator, describing the optical signal transformation by spectral device is obtained. On the basis of the proposed alternative theory the possibility of using the diffraction grating spectral device for calculation of convolution and correlation of optical pulse signals is showed.

  11. Coupling 3D Monte Carlo light transport in optically heterogeneous tissues to photoacoustic signal generation

    PubMed Central

    Jacques, Steven L.

    2014-01-01

    The generation of photoacoustic signals for imaging objects embedded within tissues is dependent on how well light can penetrate to and deposit energy within an optically absorbing object, such as a blood vessel. This report couples a 3D Monte Carlo simulation of light transport to stress wave generation to predict the acoustic signals received by a detector at the tissue surface. The Monte Carlo simulation allows modeling of optically heterogeneous tissues, and a simple MATLAB™ acoustic algorithm predicts signals reaching a surface detector. An example simulation considers a skin with a pigmented epidermis, a dermis with a background blood perfusion, and a 500-μm-dia. blood vessel centered at a 1-mm depth in the skin. The simulation yields acoustic signals received by a surface detector, which are generated by a pulsed 532-nm laser exposure before and after inserting the blood vessel. A MATLAB™ version of the acoustic algorithm and a link to the 3D Monte Carlo website are provided. PMID:25426426

  12. Coupling 3D Monte Carlo light transport in optically heterogeneous tissues to photoacoustic signal generation.

    PubMed

    Jacques, Steven L

    2014-12-01

    The generation of photoacoustic signals for imaging objects embedded within tissues is dependent on how well light can penetrate to and deposit energy within an optically absorbing object, such as a blood vessel. This report couples a 3D Monte Carlo simulation of light transport to stress wave generation to predict the acoustic signals received by a detector at the tissue surface. The Monte Carlo simulation allows modeling of optically heterogeneous tissues, and a simple MATLAB™ acoustic algorithm predicts signals reaching a surface detector. An example simulation considers a skin with a pigmented epidermis, a dermis with a background blood perfusion, and a 500-μm-dia. blood vessel centered at a 1-mm depth in the skin. The simulation yields acoustic signals received by a surface detector, which are generated by a pulsed 532-nm laser exposure before and after inserting the blood vessel. A MATLAB™ version of the acoustic algorithm and a link to the 3D Monte Carlo website are provided.

  13. Analysis of the modulation impairments in optical sideband injection locking for millimeter-wave signal generation

    NASA Astrophysics Data System (ADS)

    Aldaya, Ivan; Campuzano, Gabriel; Castañón, Gerardo

    2014-03-01

    In optical sideband injection locking (OSBIL) transmitters, the millimeter-wave (mm-wave) signal is generated heterodyning the phase-correlated outputs of two lasers, resulting in mm-wave signals with high frequency purity. Additionally, OSBIL is a cost-effective mm-wave generation technique since it does not require any external broad-bandwidth optical modulator. However, the non-linear distortion and the noise of the directly modulated laser limit its performance. This paper studies for the first time, the modulation impairments in terms of the injection conditions considering the sideband asymmetry that persists in heterodyne generation techniques. Signal-to-noise ratio and nonlinear distortion are analyzed through simulations showing different performance for upper and lower modulation sidebands and its dependence on the intermediate frequency. We show that the noise spectral density is reduced setting up the injected laser at (i) low power injection ratios and using a low intermediate frequency or (ii) higher power injection ratios, a negative frequency detuning, and a higher intermediate frequency. Regarding nonlinearities, we show that its effect on the lower modulation sideband is less significant than in the upper sideband. This impairment analysis is used to optimize the generation of OFDM signals in the 60-GHz band, achieving 2.5 Gbps generation with a spectral efficiency of 3.4 bps/Hz.

  14. Fibre segment interferometry using code-division multiplexed optical signal processing for strain sensing applications

    NASA Astrophysics Data System (ADS)

    Kissinger, Thomas; Charrett, Thomas O. H.; Tatam, Ralph P.

    2013-09-01

    A novel optical signal processing scheme for multiplexing fibre segment interferometers is proposed. The continuous-wave, homodyne technique combines code-division multiplexing with single-sideband modulation. It uses only one electro-optic phase modulator to achieve both range separation and quadrature interferometric phase measurement. This scheme is applied to fibre segment interferometry, where a number of long-gauge length interferometric fibre sensors are formed by subtracting pairs of signals from equidistantly placed, weak back reflectors. In this work we give a detailed account of the signal processing involved and, in particular, explore aspects such as electronic bandwidth requirements, noise, crosstalk and linearity, which are important design considerations. A signal bandwidth of ±20 kHz permits the resolution of phase change rates of 2.5 × 104 rad s-1 for each of the four 16.5 m long segments in our setup. We show that dynamic strain resolutions below 0.2 nanostrain Hz-0.5 at 2 m sensor gauge length are achievable, even with an inexpensive diode laser. When used in applications that require only relative strain change measurements, this scheme compares well to more established techniques and can provide high-fidelity yet cost-effective measurements.

  15. Optical recording of signal-mediated protein transport through single nuclear pore complexes.

    PubMed

    Keminer, O; Siebrasse, J P; Zerf, K; Peters, R

    1999-10-12

    Optical single-transporter recording, a recently established fluorescence microscopic method, was used to study the selective transport of proteins through single nuclear pore complexes (NPCs) of Xenopus oocytes. Recombinant proteins containing either a nuclear localization signal (import protein) or a nuclear export signal (export protein) were generated as transport substrates. To approximate in vivo conditions as closely as possible, a Xenopus egg extract was applied to the cytosolic side and a Xenopus oocyte nuclear extract to the nuclear side of the NPCs. It was found that protein transport through functionally isolated, "patched" NPCs depended on signal sequences, extracts, and metabolic energy, as in vivo. All NPCs were competent for both import and export. The transport direction was strictly determined by the transport signal, and at none of the conditions explored was the import protein exported or the export protein imported, even when the application sides of the extracts were reversed. The mean transport rates of the single NPC were approximately 2 dimers/s for the import protein and approximately 4 dimers/s for the export protein ( approximately 15 microM substrate concentration, 22-24 degrees C), in good agreement with in vivo rates estimated for mammalian cells by microinjection experiments. The study shows that optical single-transporter recording permits the analysis of membrane transport processes not previously accessible to single-transporter recording and thus provides additional possibilities for the elucidation of nucleocytoplasmic transport mechanisms. PMID:10518538

  16. Optical recording of signal-mediated protein transport through single nuclear pore complexes

    PubMed Central

    Keminer, Oliver; Siebrasse, Jan-Peter; Zerf, Katja; Peters, Reiner

    1999-01-01

    Optical single-transporter recording, a recently established fluorescence microscopic method, was used to study the selective transport of proteins through single nuclear pore complexes (NPCs) of Xenopus oocytes. Recombinant proteins containing either a nuclear localization signal (import protein) or a nuclear export signal (export protein) were generated as transport substrates. To approximate in vivo conditions as closely as possible, a Xenopus egg extract was applied to the cytosolic side and a Xenopus oocyte nuclear extract to the nuclear side of the NPCs. It was found that protein transport through functionally isolated, “patched” NPCs depended on signal sequences, extracts, and metabolic energy, as in vivo. All NPCs were competent for both import and export. The transport direction was strictly determined by the transport signal, and at none of the conditions explored was the import protein exported or the export protein imported, even when the application sides of the extracts were reversed. The mean transport rates of the single NPC were ≈2 dimers/s for the import protein and ≈4 dimers/s for the export protein (≈15 μM substrate concentration, 22–24°C), in good agreement with in vivo rates estimated for mammalian cells by microinjection experiments. The study shows that optical single-transporter recording permits the analysis of membrane transport processes not previously accessible to single-transporter recording and thus provides additional possibilities for the elucidation of nucleocytoplasmic transport mechanisms. PMID:10518538

  17. An investigation of the influence of residual amplitude modulation in phase electro-optic modulator on the signal of fiber-optic gyroscope

    NASA Astrophysics Data System (ADS)

    Pogorelaya, D. A.; Smolovik, M. A.; Strigalev, V. E.; Aleynik, A. S.; Deyneka, I. G.

    2016-08-01

    The investigation is devoted to residual amplitude modulation (RAM) of phase electro-optic modulator, which guides are made in LiNbO3 crystal by Ti diffusion technology. An analysis is presented that shows influence of RAM on the signal of fiber-optic gyroscope. The RAM compensation method is offered.

  18. Optical signal processing of video surveillance for recognizing and measurement location railway infrastructure elements

    NASA Astrophysics Data System (ADS)

    Diyazitdinov, Rinat R.; Vasin, Nikolay N.

    2016-03-01

    Processing of optical signals, which are received from CCD sensors of video cameras, allows to extend the functionality of video surveillance systems. Traditional video surveillance systems are used for saving, transmitting and preprocessing of the video content from the controlled objects. Video signal processing by analytics systems allows to get more information about object's location and movement, the flow of technological processes and to measure other parameters. For example, the signal processing of video surveillance systems, installed on carriage-laboratories, are used for getting information about certain parameters of the railways. Two algorithms for video processing, allowing recognition of pedestrian crossings of the railways, as well as location measurement of the so-called "Anchor Marks" used to control the mechanical stresses of continuous welded rail track are described in this article. The algorithms are based on the principle of determining the region of interest (ROI), and then the analysis of the fragments inside this ROI.

  19. Jitter model and signal processing techniques for pulse width modulation optical recording

    NASA Technical Reports Server (NTRS)

    Liu, Max M.-K.

    1991-01-01

    A jitter model and signal processing techniques are discussed for data recovery in Pulse Width Modulation (PWM) optical recording. In PWM, information is stored through modulating sizes of sequential marks alternating in magnetic polarization or in material structure. Jitter, defined as the deviation from the original mark size in the time domain, will result in error detection if it is excessively large. A new approach is taken in data recovery by first using a high speed counter clock to convert time marks to amplitude marks, and signal processing techniques are used to minimize jitter according to the jitter model. The signal processing techniques include motor speed and intersymbol interference equalization, differential and additive detection, and differential and additive modulation.

  20. All-optical non-conjugated wavelength multicasting of QPSK signal with capability of phase regeneration.

    PubMed

    Lian, Junzi; Fu, Songnian; Meng, Yan; Tang, Ming; Shum, Perry; Liu, Deming

    2014-09-22

    We propose all-optical one-to-three non-conjugated wavelength multicasting of QPSK signal with capability of phase regeneration, using dual-conjugated-pump phase sensitive amplification (PSA). Based on the seven-wave model, we can obtain phase transfer functions of individual multicasting channel. Different from two multicasting copies, the phase regeneration performance of input signal is determined by the nonlinear phase shift. Moreover, the optimal squeezing points of three multicasting channels have a deviation. Thus, there exists a regeneration performance trade-off among three multicasting channels. Our numerical simulation shows that the error vector magnitude (EVM) of 50 Gb/s QPSK signal can be successfully improved when both nonlinear phase shift and four-state position in its constellation are optimized. The calculated BER curves verify that the OSNR penalties of three multicasting channels are improved by around 1dB at BER = 10(-3).

  1. Prototype positron emission tomography insert with electro-optical signal transmission for simultaneous operation with MRI.

    PubMed

    Olcott, Peter; Kim, Ealgoo; Hong, Keyjo; Lee, Brian J; Grant, Alexander M; Chang, Chen-Ming; Glover, Gary; Levin, Craig S

    2015-05-01

    The simultaneous acquisition of PET and MRI data shows promise to provide powerful capabilities to study disease processes in human subjects, guide the development of novel treatments, and monitor therapy response and disease progression. A brain-size PET detector ring insert for an MRI system is being developed that, if successful, can be inserted into any existing MRI system to enable simultaneous PET and MRI images of the brain to be acquired without mutual interference. The PET insert uses electro-optical coupling to relay all the signals from the PET detectors out of the MRI system using analog modulated lasers coupled to fiber optics. Because the fibers use light instead of electrical signals, the PET detector can be electrically decoupled from the MRI making it partially transmissive to the RF field of the MRI. The SiPM devices and low power lasers were powered using non-magnetic MRI compatible batteries. Also, the number of laser-fiber channels in the system was reduced using techniques adapted from the field of compressed sensing. Using the fact that incoming PET data is sparse in time and space, electronic circuits implementing constant weight codes uniquely encode the detector signals in order to reduce the number of electro-optical readout channels by 8-fold. Two out of a total of sixteen electro-optical detector modules have been built and tested with the entire RF-shielded detector gantry for the PET ring insert. The two detectors have been tested outside and inside of a 3T MRI system to study mutual interference effects and simultaneous performance with MRI. Preliminary results show that the PET insert is feasible for high resolution simultaneous PET/MRI imaging for applications in the brain. PMID:25856511

  2. Prototype positron emission tomography insert with electro-optical signal transmission for simultaneous operation with MRI

    NASA Astrophysics Data System (ADS)

    Olcott, Peter; Kim, Ealgoo; Hong, Keyjo; Lee, Brian J.; Grant, Alexander M.; Chang, Chen-Ming; Glover, Gary; Levin, Craig S.

    2015-05-01

    The simultaneous acquisition of PET and MRI data shows promise to provide powerful capabilities to study disease processes in human subjects, guide the development of novel treatments, and monitor therapy response and disease progression. A brain-size PET detector ring insert for an MRI system is being developed that, if successful, can be inserted into any existing MRI system to enable simultaneous PET and MRI images of the brain to be acquired without mutual interference. The PET insert uses electro-optical coupling to relay all the signals from the PET detectors out of the MRI system using analog modulated lasers coupled to fiber optics. Because the fibers use light instead of electrical signals, the PET detector can be electrically decoupled from the MRI making it partially transmissive to the RF field of the MRI. The SiPM devices and low power lasers were powered using non-magnetic MRI compatible batteries. Also, the number of laser-fiber channels in the system was reduced using techniques adapted from the field of compressed sensing. Using the fact that incoming PET data is sparse in time and space, electronic circuits implementing constant weight codes uniquely encode the detector signals in order to reduce the number of electro-optical readout channels by 8-fold. Two out of a total of sixteen electro-optical detector modules have been built and tested with the entire RF-shielded detector gantry for the PET ring insert. The two detectors have been tested outside and inside of a 3T MRI system to study mutual interference effects and simultaneous performance with MRI. Preliminary results show that the PET insert is feasible for high resolution simultaneous PET/MRI imaging for applications in the brain.

  3. Multiplexed optical operation of nanoelectromechanical systems (NEMS) arrays for sensing and signal-processing applications

    NASA Astrophysics Data System (ADS)

    Sampathkumar, Ashwin

    2014-06-01

    NEMS are rapidly being developed for a variety of sensing applications as well as for exploring interesting regimes in fundamental physics. In most of these endeavors, operation of a NEMS device involves actuating the device harmonically around its fundamental resonance and detecting subsequent motion while the device interacts with its environment. Even though a single NEMS resonator is exceptionally sensitive, a typical application, such as sensing or signal processing, requires the detection of signals from many resonators distributed over the surface of a chip. Therefore, one of the key technological challenges in the field of NEMS is development of multiplexed measurement techniques to detect the motion of a large number of NEMS resonators simultaneously. In this work, we address the important and difficult problem of interfacing with a large number of NEMS devices and facilitating the use of such arrays in, for example, sensing and signal processing applications. We report a versatile, all-optical technique to excite and read-out a distributed NEMS array. The NEMS array is driven by a distributed, intensity-modulated, optical pump through the photothermal effect. The ensuing vibrational response of the array is multiplexed onto a single, probe beam as a high-frequency phase modulation. The phase modulation is optically down converted to a low-frequency, intensity modulation using an adaptive full -field interferometer, and subsequently is detected using a charge-coupled device (CCD) array. Rapid and single-step mechanical characterization of approximately 60 nominally identical, high-frequency resonators is demonstrated. The technique may enable sensitivity improvements over single NEMS resonators by averaging signals coming from a multitude of devices in the array. In addition, the diffraction-limited spatial resolution may allow for position-dependent read-out of NEMS sensor chips for sensing multiple analytes or spatially inhomogeneous forces.

  4. Ground Optical Signal Processing Architecture for Contributing SSA Space Based Sensor Data

    NASA Astrophysics Data System (ADS)

    Koblick, D.; Klug, M.; Goldsmith, A.; Flewelling, B.; Jah, M.; Shanks, J.; Piña, R.

    2014-09-01

    The main objective of the DARPA program Orbit Outlook (O^2) is to improve the metric tracking and detection performance of the Space Situational Network (SSN) by adding a diverse low-cost network of contributing sensors to the Space Situational Awareness (SSA) mission. In order to accomplish this objective, not only must a sensor be in constant communication with a planning and scheduling system to process tasking requests, there must be an underlying framework to provide useful data products, such as angles only measurements. Existing optical signal processing implementations such as the Optical Processing Architecture at Lincoln (OPAL) are capable of converting mission data collections to angles only observations, but may be difficult for many users to obtain, support, and customize for low-cost missions and demonstration programs. The Ground Optical Signal Processing Architecture (GOSPA) will ingest raw imagery and telemetry data from a space based electro optical sensor and perform a background removal process to remove anomalous pixels, interpolate over bad pixels, and dominant temporal noise. After background removal, the streak end points and target centroids are located using a corner detection algorithm developed by Air Force Research Laboratory. These identified streak locations are then fused with the corresponding spacecraft telemetry data to determine the Right Ascension and Declination measurements with respect to time. To demonstrate the performance of GOSPA, non-rate tracking collections against a satellite in Geosynchronous Orbit are simulated from a visible optical imaging sensor in a polar Low Earth Orbit. Stars, noise and bad pixels are added to the simulated images based on look angles and sensor parameters. These collections are run through the GOSPA framework to provide angles- only measurements to the Air Force Research Laboratory Constrained Admissible Region Multiple Hypothesis Filter (CAR-MHF) in which an Initial Orbit Determination is

  5. The distribution of highly stable millimeter-wave signals over different optical fiber links with accurate phase-correction

    NASA Astrophysics Data System (ADS)

    Liu, Zhangweiyi; Wang, Xiaocheng; Sun, Dongning; Dong, Yi; Hu, Weisheng

    2015-08-01

    We have demonstrated an optical generation of highly stable millimeter-wave signal distribution system, which transfers a 300GHz signal to two remote ends over different optical fiber links for signal stability comparison. The transmission delay variations of each fiber link caused by temperature and mechanical perturbations are compensated by high-precise phase-correction system. The residual phase noise between two remote end signals is detected by dual-heterodyne phase error transfer and reaches -46dBc/Hz at 1 Hz frequency offset from the carrier. The relative instability is 8×10-17 at 1000s averaging time.

  6. Simulation of optical configurations and signal processing methods in Anger-type neutron-position scintillation detector

    SciTech Connect

    Roche, C.T.; Strauss, M.G.; Brenner, R.

    1984-01-01

    The spatial linearity and resolution of Anger-type neutron-position scintillation detectors are studied using a semi-empirical model. Detector optics with either an air gap or optical grease between the scintillator and the dispersive light guide are considered. Three signal processing methods which truncate signals from PMT's distant from the scintillation are compared with the linear resistive weighting method. Air gap optics yields a 15% improvement in spatial resolution and 50% reduction in differential and integral nonlinearity relative to grease coupled optics, using linear processing. Using signal truncation instead of linear processing improves the resolution 15-20% for the air gap and 20-30% for the grease coupling case. Thus, the initial discrepancy in the resolution between the two optics nearly vanished, however the linearity of the grease coupled system is still significantly poorer.

  7. Resolution and linearity of Anger-type neutron-position detectors as simulated with different signal processing and optics

    SciTech Connect

    Roche, C.T.; Brenner, R.; Strauss, M.G.

    1985-02-01

    The apatial linearity and resolution of Anger-type neutron-position scintillation detectors are studied using a semi-empirical model. Detector optics with either an air gap or optical grease between the scintillator and the dispersive light guide are considered. An air gap focuses the scintillation light on the photomultiplier tubes nearest the scintillation point. Four signal processing methods which truncate signals from photomultipler tubes distant from the scintillation are compared with the linear resistive weighting method. Using linear processing, air-gap optics yield a 25% improvement in resolution distance and an 80% reduction in integral nonlinearity relative to grease-coupled optics. With either optics, using signal truncation instead of linear processing improves the resolution distance 5-15%.

  8. Simulation of optical configurations and signal processing methods in Anger-type neutron-position scintillation detector

    NASA Astrophysics Data System (ADS)

    Roche, C. T.; Strauss, M. G.; Brenner, R.

    The spatial linearity and resolution of Anger type neutron position scintillation detectors were studied as a semi-empirical model. Detector optics with either an air gap or optical grease between the scintillator and the dispersive light guide are considered. Three signal processing methods which truncate signals from PMT's distant from the scintillation are compared with the linear resistive weighting method. Air gap optics yields a 15% improvement in spatial resolution and 50% reduction in differential and integral nonlinearity relative to grease coupled optics, using linear processing. Using signal truncation instead of linear processing improves the resolution 15% to 20% for the air gap and 20% to 30% for the grease coupling case. The initial discrepancy in the resolution between the two optics nearly vanished, however, the linearity of the grease coupled system is still significantly poorer.

  9. Coherent demodulation of microwave signals by using optical heterodyne technique with applications to point to point indoor wireless communications systems

    NASA Astrophysics Data System (ADS)

    García-Juárez, A.; Zaldívar-Huerta, I. E.; Aguayo-Rodríguez, G.; Rodríguez-Asomoza, J.; Gómez-Colín, M. R.; Rojas-Hernández, A. G.

    2011-01-01

    An optical communications system using a couple microstrip antennas for distributing point to point analog TV with coherent demodulation based on optical heterodyne in close vicinity is reported in this paper. In the proposed experimental setup, two optical waves at different wavelengths are mixed and applied to a photodetector. Then a beat signal with a frequency equivalent to the spacing of the two wavelengths is obtained at the output of the photodetector. This signal corresponds to a microwave signal located at 1.25 GHz, which it is used as a microwave carrier in the transmitter and as a local oscillator in the receiver of our optical communication system. The feasibility of this technique is demonstrated transmitting a TV signal of 66-72MHz.

  10. AMA- and RWE- Based Adaptive Kalman Filter for Denoising Fiber Optic Gyroscope Drift Signal

    PubMed Central

    Yang, Gongliu; Liu, Yuanyuan; Li, Ming; Song, Shunguang

    2015-01-01

    An improved double-factor adaptive Kalman filter called AMA-RWE-DFAKF is proposed to denoise fiber optic gyroscope (FOG) drift signal in both static and dynamic conditions. The first factor is Kalman gain updated by random weighting estimation (RWE) of the covariance matrix of innovation sequence at any time to ensure the lowest noise level of output, but the inertia of KF response increases in dynamic condition. To decrease the inertia, the second factor is the covariance matrix of predicted state vector adjusted by RWE only when discontinuities are detected by adaptive moving average (AMA).The AMA-RWE-DFAKF is applied for denoising FOG static and dynamic signals, its performance is compared with conventional KF (CKF), RWE-based adaptive KF with gain correction (RWE-AKFG), AMA- and RWE- based dual mode adaptive KF (AMA-RWE-DMAKF). Results of Allan variance on static signal and root mean square error (RMSE) on dynamic signal show that this proposed algorithm outperforms all the considered methods in denoising FOG signal. PMID:26512665

  11. AMA- and RWE- Based Adaptive Kalman Filter for Denoising Fiber Optic Gyroscope Drift Signal.

    PubMed

    Yang, Gongliu; Liu, Yuanyuan; Li, Ming; Song, Shunguang

    2015-10-23

    An improved double-factor adaptive Kalman filter called AMA-RWE-DFAKF is proposed to denoise fiber optic gyroscope (FOG) drift signal in both static and dynamic conditions. The first factor is Kalman gain updated by random weighting estimation (RWE) of the covariance matrix of innovation sequence at any time to ensure the lowest noise level of output, but the inertia of KF response increases in dynamic condition. To decrease the inertia, the second factor is the covariance matrix of predicted state vector adjusted by RWE only when discontinuities are detected by adaptive moving average (AMA).The AMA-RWE-DFAKF is applied for denoising FOG static and dynamic signals, its performance is compared with conventional KF (CKF), RWE-based adaptive KF with gain correction (RWE-AKFG), AMA- and RWE- based dual mode adaptive KF (AMA-RWE-DMAKF). Results of Allan variance on static signal and root mean square error (RMSE) on dynamic signal show that this proposed algorithm outperforms all the considered methods in denoising FOG signal.

  12. Brain mapping: new wave optical imaging.

    PubMed

    Mrsic-Flogel, Thomas; Hübener, Mark; Bonhoeffer, Tobias

    2003-09-30

    Optical imaging of intrinsic signals is widely used for high-resolution brain mapping in various animal species. A new approach using continuous data acquisition and Fourier decomposition of the signal allows for much faster mapping, opening up the possibility of applying this method to new experimental questions. PMID:14521859

  13. Electronic polarization-division demultiplexing based on digital signal processing in intensity-modulation direct-detection optical communication systems.

    PubMed

    Kikuchi, Kazuro

    2014-01-27

    We propose a novel configuration of optical receivers for intensity-modulation direct-detection (IM · DD) systems, which can cope with dual-polarization (DP) optical signals electrically. Using a Stokes analyzer and a newly-developed digital signal-processing (DSP) algorithm, we can achieve polarization tracking and demultiplexing in the digital domain after direct detection. Simulation results show that the power penalty stemming from digital polarization manipulations is negligibly small.

  14. Effect of resin type on the signal integrity of an embedded perfluorinated polymer optical fiber

    NASA Astrophysics Data System (ADS)

    Hamouda, Tamer; Peters, Kara; Seyam, Abdel-Fattah M.

    2012-05-01

    Polymer optical fibers (POF) hold many advantages for embedded sensing, such as their low cost, flexibility, high tensile strain limits and high fracture toughness. POF sensors may therefore be integrated into fiber reinforced composite structures for monitoring structural behavior. Since POFs do not require a protective coating, it is critical to verify that the resin system does not have a negative impact on the noise level or performance of POF sensors during composite manufacture. This study measured the effect of vinylester and epoxy resin systems on the signal loss of embedded perfluorinated, graded index POFs. Photon-counting optical time domain reflectometry (OTDR) was used to monitor the signal attenuation and backscattering level of the POFs throughout the resin curing cycle. Fourier transform infrared spectrometry (FTIR) and cross section analyses using scanning electronic microscope (SEM) images were also conducted to investigate whether the resin system caused chemical and physical changes of the POF. This study showed that vinylester resin caused a significant increase in the backscattering level of POF sensors and therefore induced high fiber signal losses. On the other hand, the POF treated with epoxy showed no change in backscattering level, indicating that no chemical or physical change had occurred to the POF.

  15. cAMP signaling microdomains and their observation by optical methods

    PubMed Central

    Calebiro, Davide; Maiellaro, Isabella

    2014-01-01

    The second messenger cyclic AMP (cAMP) is a major intracellular mediator of many hormones and neurotransmitters and regulates a myriad of cell functions, including synaptic plasticity in neurons. Whereas cAMP can freely diffuse in the cytosol, a growing body of evidence suggests the formation of cAMP gradients and microdomains near the sites of cAMP production, where cAMP signals remain apparently confined. The mechanisms responsible for the formation of such microdomains are subject of intensive investigation. The development of optical methods based on fluorescence resonance energy transfer (FRET), which allow a direct observation of cAMP signaling with high temporal and spatial resolution, is playing a fundamental role in elucidating the nature of such microdomains. Here, we will review the optical methods used for monitoring cAMP and protein kinase A (PKA) signaling in living cells, providing some examples of their application in neurons, and will discuss the major hypotheses on the formation of cAMP/PKA microdomains. PMID:25389388

  16. Low-noise and high-gain Brillouin optical amplifier for narrowband active optical filtering based on a pump-to-signal optoelectronic tracking.

    PubMed

    Souidi, Yahia; Taleb, Fethallah; Zheng, Junbo; Lee, Min Won; Du Burck, Frédéric; Roncin, Vincent

    2016-01-10

    We implement and characterize an optical narrowband amplifier based on stimulated Brillouin scattering with pump-to-signal relative frequency fluctuations overcome thanks to an active pump tracking. We achieve a precise characterization of this amplifier in terms of gain and noise degradation (noise figure). The performances of this stable selective amplification are compared to those of a conventional erbium-doped fiber amplifier in order to highlight the interest of the Brillouin amplification solution for active narrow optical filtering with a bandpass of 10 MHz. Thanks to the simple optoelectronic pump-to-signal tracking, the Brillouin active filter appears as a stable and reliable solution for narrowband optical processing in the coherent optical communication context and optical sensor applications. PMID:26835759

  17. Coherent optical three-dimensional spectrum-correlation processing of wave signals based on space-time integration.

    PubMed

    Ezhov, Vasily

    2012-11-20

    The architectures of classical analog coherent optical (ACO) spectrum analyzers and correlators are not designed to process the wave signal as a whole, i.e., simultaneously in three dimensions. In this paper, the theory of ACO three-dimensional direct spectrum-correlation processing of spatial-temporal optical replicas (copies) of wave signals is discussed. In the single-stage and two-stage ACO systems, the spatial power spectrum and spatial correlation function of the wave signal (envelope) are obtained on the basis of space-time integration. The geometry of the final compressed signal in the output plane of either optical system allows one to evaluate the angle of wave arrival. The wave signal to be processed can theoretically have any form (due to autocorrelation properties of the systems) and an unlimited duration (due to time integration of wave energy and possibility of electronic subtraction of the intermediate bias terms of the time integration).

  18. Optical hybrid analog-digital signal processing based on spike processing in neurons

    NASA Astrophysics Data System (ADS)

    Fok, Mable P.; Tian, Yue; Rosenbluth, David; Deng, Yanhua; Prucnal, Paul R.

    2011-09-01

    Spike processing is one kind of hybrid analog-digital signal processing, which has the efficiency of analog processing and the robustness to noise of digital processing. When instantiated with optics, a hybrid analog-digital processing primitive has the potential to be scalable, computationally powerful, and have high operation bandwidth. These devices open up a range of processing applications for which electronic processing is too slow. Our approach is based on a hybrid analog/digital computational primitive that elegantly implements the functionality of an integrate-and-fire neuron using a Ge-doped non-linear optical fiber and off-the-shelf semiconductor devices. In this paper, we introduce our photonic neuron architecture and demonstrate the feasibility of implementing simple photonic neuromorphic circuits, including the auditory localization algorithm of the barn owl, which is useful for LIDAR localization, and the crayfish tail-flip escape response.

  19. Novel optical methodologies in studying mechanical signal transduction in mammalian cells

    NASA Technical Reports Server (NTRS)

    Stamatas, G. N.; McIntire, L. V.

    1999-01-01

    For the last 3 decades evidence has been accumulating that some types of mammalian cells respond to their mechanically active environment by altering their morphology, growth rate, and metabolism. The study of such responses is very important in understanding, physiological and pathological conditions ranging from bone formation to atherosclerosis. Obtaining this knowledge has been the goal for an active research area in bioengineering termed cell mechanotransduction. The advancement of optical methodologies used in cell biology research has given the tools to elucidate cellular mechanisms that would otherwise be impossible to visualize. Combined with molecular biology techniques, they give engineers invaluable tools in understanding the chemical pathways involved in mechanotransduction. Herein we briefly review the current knowledge on mechanical signal transduction in mammalian cells, focusing on the application of novel optical techniques in the ongoing research.

  20. Nonlinear silicon-on-insulator waveguides for all-optical signal processing

    NASA Astrophysics Data System (ADS)

    Koos, C.; Jacome, L.; Poulton, C.; Leuthold, J.; Freude, W.

    2007-05-01

    Values up to γ=7×106/(Wkm) for the nonlinear parameter are feasible if silicon-on-insulator based strip and slot waveguides are properly designed. This is more than three orders of magnitude larger than for state-of-the-art highly nonlinear fibers, and it enables ultrafast all-optical signal processing with nonresonant compact devices. At λ=1.55μm we provide universal design curves for strip and slot waveguides which are covered with different linear and nonlinear materials, and we calculate the resulting maximum γ.

  1. A digital-signal-processor-based optical tomographic system for dynamic imaging of joint diseases

    NASA Astrophysics Data System (ADS)

    Lasker, Joseph M.

    Over the last decade, optical tomography (OT) has emerged as viable biomedical imaging modality. Various imaging systems have been developed that are employed in preclinical as well as clinical studies, mostly targeting breast imaging, brain imaging, and cancer related studies. Of particular interest are so-called dynamic imaging studies where one attempts to image changes in optical properties and/or physiological parameters as they occur during a system perturbation. To successfully perform dynamic imaging studies, great effort is put towards system development that offers increasingly enhanced signal-to-noise performance at ever shorter data acquisition times, thus capturing high fidelity tomographic data within narrower time periods. Towards this goal, I have developed in this thesis a dynamic optical tomography system that is, unlike currently available analog instrumentation, based on digital data acquisition and filtering techniques. At the core of this instrument is a digital signal processor (DSP) that collects, collates, and processes the digitized data set. Complementary protocols between the DSP and a complex programmable logic device synchronizes the sampling process and organizes data flow. Instrument control is implemented through a comprehensive graphical user interface which integrates automated calibration, data acquisition, and signal post-processing. Real-time data is generated at frame rates as high as 140 Hz. An extensive dynamic range (˜190 dB) accommodates a wide scope of measurement geometries and tissue types. Performance analysis demonstrates very low system noise (˜1 pW rms noise equivalent power), excellent signal precision (˜0.04%--0.2%) and long term system stability (˜1% over 40 min). Experiments on tissue phantoms validate spatial and temporal accuracy of the system. As a potential new application of dynamic optical imaging I present the first application of this method to use vascular hemodynamics as a means of characterizing

  2. A Sagnac-Michelson fibre optic interferometer: Signal processing for disturbance localization

    NASA Astrophysics Data System (ADS)

    Kondrat, M.; Szustakowski, M.; Pałka, N.; Ciurapiński, W.; Życzkowski, M.

    2007-09-01

    We present numerical and experimental results on a new generation fibre optic perimeter sensor based on a Sagnac and Michelson interferometers configuration. In particular, an original signal processing scheme is presented. The sensor can detect a potential intruder and determine its position along a protected zone. We propose a localization method that offers the inherent properties of both interferometers. After demodulation of the signals from both interferometers, the obtained amplitude characteristic of the Sagnac interferometer depends on a position of a disturbance along the interferometer, while amplitude characteristic of the Michelson interferometer does not depend on this position. So, quotient of both demodulated characteristics makes it possible to localize the disturbance. During investigations of a laboratory model of the sensor, it was possible to detect the position of the disturbance with a resolution of about 40 m along the 6-km-long sensor.

  3. Gigabit Ethernet signal transmission using asynchronous optical code division multiple access.

    PubMed

    Ma, Philip Y; Fok, Mable P; Shastri, Bhavin J; Wu, Ben; Prucnal, Paul R

    2015-12-15

    We propose and experimentally demonstrate a novel architecture for interfacing and transmitting a Gigabit Ethernet (GbE) signal using asynchronous incoherent optical code division multiple access (OCDMA). This is the first such asynchronous incoherent OCDMA system carrying GbE data being demonstrated to be working among multi-users where each user is operating with an independent clock/data rate and is granted random access to the network. Three major components, the GbE interface, the OCDMA transmitter, and the OCDMA receiver are discussed in detail. The performance of the system is studied and characterized through measuring eye diagrams, bit-error rate and packet loss rate in real-time file transfer. Our Letter also addresses the near-far problem and realizes asynchronous transmission and detection of signal.

  4. 50 Mbps free space direct detection laser diode optical communication system with Q = 4 PPM signaling

    NASA Technical Reports Server (NTRS)

    Sun, Xiaoli; Davidson, Frederic; Field, Christopher

    1990-01-01

    A 50 Mbps direct detection optical communication system for use in an intersatellite link was constructed with an AlGaAs laser diode transmitter and a silicon avalanche photodiode photodetector. The system used a Q = 4 PPM format. The receiver consisted of a maximum likelihood PPM detector and a timing recovery subsystem. The PPM slot clock was recovered at the receiver by using a transition detector followed by a PLL. The PPM word clock was recovered by using a second PLL whose input was derived from the presence of back-to-back PPM pulses contained in the received random PPM pulse sequences. The system achieved a bit error rate of 0.000001 at less than 50 detected signal photons/information bit. The receiver was capable of acquiring and maintaining slot and word synchronization for received signal levels greater than 20 photons/information bit, at which the receiver bit error rate was about 0.01.

  5. Matching visual and nonvisual signals: evidence for a mechanism to discount optic flow during locomotion

    NASA Astrophysics Data System (ADS)

    Thurrell, Adrian; Pelah, Adar

    2005-03-01

    We report on recent experiments to investigate the Arthrovisual Locomotor Effect (ALE), a mechanism based on non-visual signals postulated to discount or remove the self-generated visual motion signals during locomotion. It is shown that perceptual matches made by standing subjects to a constant motion optic flow stimulus that is viewed while walking on a treadmill are linearly reduced by walking speed, a measure of the reported ALE. The degree of reduction in perceived speed depends on the similarity of the motor activity to natural locomotion, thus for the four activities tested, ALE strength is ranked as follows: Walking > Cycling > Hand Pedalling > Finger Tapping = 0. Other variations and important controls for the ALE are described.

  6. Studying the distribution of deep Raman spectroscopy signals using liquid tissue phantoms with varying optical properties.

    PubMed

    Vardaki, Martha Z; Gardner, Benjamin; Stone, Nicholas; Matousek, Pavel

    2015-08-01

    In this study we employed large volume liquid tissue phantoms, consisting of a scattering agent (Intralipid), an absorption agent (Indian ink) and a synthesized calcification powder (calcium hydroxyapatite (HAP)) similar to that found in cancerous tissues (e.g. breast and prostate), to simulate human tissues. We studied experimentally the magnitude and origin of Raman signals in a transmission Raman geometry as a function of optical properties of the medium and the location of calcifications within the phantom. The goal was to inform the development of future noninvasive cancer screening applications in vivo. The results provide insight into light propagation and Raman scattering distribution in deep Raman measurements, exploring also the effect of the variation of relative absorbance of laser and Raman photons within the phantoms. Most notably when modeling breast and prostate tissues it follows that maximum signals is obtained from the front and back faces of the tissue with the central region contributing less to the measured spectrum.

  7. Coherent Optical Focal Plane Array Receiver for PPM Signals: Investigation and Applications

    NASA Technical Reports Server (NTRS)

    Fernandez, Michela Munoz

    2006-01-01

    The performance of a coherent optical focal plane array receiver for PPM signals under atmospheric turbulence is investigated and applications of this system are addressed. The experimental demonstration of this project has already been explained in previous publications [1]. This article shows a more exhaustive analysis of the expressions needed to obtain the Bit Error Rate (BER) for the real system under study in the laboratory. Selected experimental results of this system are described and compared with theoretical BER expressions, and array combining gains are presented. Receiver sensitivity in terms of photons per bit (PPB) is examined; BER results are shown as a function of signal to noise ratios, (SNR), as well as a function of photons per symbol, and photons per bit.

  8. Electro-optical techniques for the investigation of photoplethysmographic signals in human abdominal organs

    NASA Astrophysics Data System (ADS)

    Kyriacou, P. A.; Crerar-Gilber, A.; Langford, R. M.; Jones, D. P.

    2006-07-01

    There is a need for reliable continuous monitoring of abdominal organ oxygen saturation (SpO2). Splanchnic ischaemia may ultimately lead to cellular hypoxia and necrosis and may well contribute to the development of multiple organ failures and increased mortality. A new reflectance electro-optical photoplethysmographic (PPG) probe and signal processing system were developed. PPG signals from abdominal organs (bowel, liver, and kidney) and the finger were obtained from 12 anaesthetised patients. The amplitudes of the abdominal organ PPGs were, on average, approximately the same as those obtained simultaneously from the finger. These observations suggest that pulse oximetry may be a valid monitoring technique for abdominal organs such as the bowel liver and kidney.

  9. Implementation theory of distortion-invariant pattern recognition for optical and digital signal processing systems

    NASA Astrophysics Data System (ADS)

    Lhamon, Michael Earl

    A pattern recognition system which uses complex correlation filter banks requires proportionally more computational effort than single-real valued filters. This introduces increased computation burden but also introduces a higher level of parallelism, that common computing platforms fail to identify. As a result, we consider algorithm mapping to both optical and digital processors. For digital implementation, we develop computationally efficient pattern recognition algorithms, referred to as, vector inner product operators that require less computational effort than traditional fast Fourier methods. These algorithms do not need correlation and they map readily onto parallel digital architectures, which imply new architectures for optical processors. These filters exploit circulant-symmetric matrix structures of the training set data representing a variety of distortions. By using the same mathematical basis as with the vector inner product operations, we are able to extend the capabilities of more traditional correlation filtering to what we refer to as "Super Images". These "Super Images" are used to morphologically transform a complicated input scene into a predetermined dot pattern. The orientation of the dot pattern is related to the rotational distortion of the object of interest. The optical implementation of "Super Images" yields feature reduction necessary for using other techniques, such as artificial neural networks. We propose a parallel digital signal processor architecture based on specific pattern recognition algorithms but general enough to be applicable to other similar problems. Such an architecture is classified as a data flow architecture. Instead of mapping an algorithm to an architecture, we propose mapping the DSP architecture to a class of pattern recognition algorithms. Today's optical processing systems have difficulties implementing full complex filter structures. Typically, optical systems (like the 4f correlators) are limited to phase

  10. State-of-the-art RF signal generation from optical frequency division.

    PubMed

    Hati, Archita; Nelson, Craig W; Barnes, Corey; Lirette, Danielle; Fortier, Tara; Quinlan, Franklyn; DeSalvo, Jason A; Ludlow, Andrew; Diddams, Scott A; Howe, David A

    2013-09-01

    We present the design of a novel, ultralow-phase-noise frequency synthesizer implemented with extremely-low-noise regenerative frequency dividers. This synthesizer generates eight outputs, viz. 1.6 GHz, 320 MHz, 160 MHz, 80 MHz, 40 MHz, 20 MHz, 10 MHz and 5 MHz for an 8 GHz input frequency. The residual single-sideband (SSB) phase noises of the synthesizer at 5 and 10 MHz outputs at 1 Hz offset from the carrier are -150 and -145 dBc/Hz, respectively, which are unprecedented phase noise levels. We also report the lowest values of phase noise to date for 5 and 10 MHz RF signals achieved with our synthesizer by dividing an 8 GHz signal generated from an ultra-stable optical-comb-based frequency division. The absolute SSB phase noises achieved for 5 and 10 MHz signals at 1 Hz offset are -150 and -143 dBc/Hz, respectively; at 100 kHz offset, they are -177 and -174 dBc/Hz, respectively. The phase noise of the 5 MHz signal corresponds to a frequency stability of approximately 7.6 × 10(-15) at 1 s averaging time for a measurement bandwidth (BW) of 500 Hz, and the integrated timing jitter over 100 kHz BW is 20 fs.

  11. Extraction of vibration parameters from optical feedback interferometry signals using wavelets.

    PubMed

    Jha, Ajit; Azcona, Francisco J; Yañez, Carlos; Royo, Santiago

    2015-12-01

    This paper proposes the use of the wavelet transform as a technique that is suited for fringe detection and analysis of optical feedback interferometry (OFI) signals, thus allowing the retrieval of extremely small physical motion phenomena. A novel algorithm based on wavelet transform is used to process the OFI signal simultaneously in the time and frequency domains, enabling precise detection of signal fringes and, thus, the extraction of amplitude features of the vibrating target with error in the order of 0.1λ. Furthermore, using a complex Morlet wavelet as an analyzing wavelet enables us to extract important information from the time envelope of the OFI signal. Such an envelope can be useful in detecting fringes even in the presence of speckle reducing the error in a displacement reconstruction. Examples of OFI applications, including nanometric displacement sensing without direction ambiguity as well as the measurement of the frequency and velocity of vibrating targets and the detection in time of no periodic events, are also presented using this wavelet approach. PMID:26836667

  12. On the optimum signal constellation design for high-speed optical transport networks.

    PubMed

    Liu, Tao; Djordjevic, Ivan B

    2012-08-27

    In this paper, we first describe an optimum signal constellation design algorithm, which is optimum in MMSE-sense, called MMSE-OSCD, for channel capacity achieving source distribution. Secondly, we introduce a feedback channel capacity inspired optimum signal constellation design (FCC-OSCD) to further improve the performance of MMSE-OSCD, inspired by the fact that feedback channel capacity is higher than that of systems without feedback. The constellations obtained by FCC-OSCD are, however, OSNR dependent. The optimization is jointly performed together with regular quasi-cyclic low-density parity-check (LDPC) code design. Such obtained coded-modulation scheme, in combination with polarization-multiplexing, is suitable as both 400 Gb/s and multi-Tb/s optical transport enabling technology. Using large girth LDPC code, we demonstrate by Monte Carlo simulations that a 32-ary signal constellation, obtained by FCC-OSCD, outperforms previously proposed optimized 32-ary CIPQ signal constellation by 0.8 dB at BER of 10(-7). On the other hand, the LDPC-coded 16-ary FCC-OSCD outperforms 16-QAM by 1.15 dB at the same BER.

  13. Influence of Multi-Cascaded Semiconductor Optical Amplifiers on the Signal in an Energy-Efficient System

    NASA Astrophysics Data System (ADS)

    Chen, Xiao-Yong; Sheng, Xin-Zhi; Wu, Chong-Qing

    2012-01-01

    Signal impairment is experimentally studied by using the extinction ratio (ER), error bit rate (BER) and optical spectrum in a three-cascaded semiconductor optical amplifier (SOA) setup. The signal with the ER of 13 dB and BER of < 10-9 is achieved after the signal passing through the cascaded SOAs. With the results obtained from the experiment, we confirm that the three-cascaded SOAs used to compensate for power in the optical transmission can be accepted. This experimental result also offers the possibility of achieving a higher throughput of multiplane architecture by exploiting three switching domains instead of two switching domains in the energy-efficient design of a scalable optical multi-plane interconnection architecture. The space switches in output ports of multi-plane interconnection architecture can be improved to N = 32 × 32 × 32 = 32768.

  14. A broadband RF continuously variable time delay device. [using Bragg cell and optical heterodyne technology for signal processing

    NASA Technical Reports Server (NTRS)

    Freyre, F. W.

    1981-01-01

    A method for implementation of continuously variable time delay of broadband RF signals is described. The method uses Bragg Cell and optical heterodyne technology. The signal to be delayed is applied to the Bragg Cell acoustic transducer, and the delay time is the acoustic transit time from this transducer to the incident light beam. By translating the light beam, the delay is varied. Expressions describing the Bragg Cell diffraction, lens Fourier transformation, and the optical heterodyne processes are developed. Specifications for the variable delay including bandwidth, range of delay, and insertion loss are provided. Applications include radar signal processing, spread spectrum intercept, radar ECM, and adaptive array antenna processing.

  15. The fractal-like complexity of heart rate variability beyond neurotransmitters and autonomic receptors: signaling intrinsic to sinoatrial node pacemaker cells

    PubMed Central

    Yaniv, Yael; Lyashkov, Alexey E.; Lakatta, Edward G.

    2015-01-01

    The heart rate and rhythm are controlled by complex chaotic neural, chemical and hormonal networks which are not strictly regular, but exhibit fluctuations across multiple time scales. A careful assessment of the heart rate variability (HRV) offers clues to this complexity. A reduction in HRV, specifically in advanced age, is associated with increase in morbidity and mortality. Mechanisms that induce this decrease, however, have not been fully elucidated. The classical literature characterizes changes in HRV as a result of changes in the balance of competing influences of the sympathetic and parasympathetic autonomic impulses delivered to the heart. It has now become clear, however, that the heart rate and HRV are also determined by intrinsic properties of the pacemaker cells that comprise sinoatrial node, and that these properties respond to autonomic receptor stimulation in a non-linear mode. That HRV is determined by both the intrinsic properties of pacemaker cells in the sinoatrial node and the competing influences of the two branches of the autonomic neural input to the cells requires an expansion of our perspective about mechanisms that govern HRV in the normal heart, and how HRV changes with aging in health and in heart diseases. PMID:26709383

  16. Comparison of cost- and energy-efficient signal modulations for next generation passive optical networks.

    PubMed

    Wei, J L; Grobe, K; Sanchez, C; Giacoumidis, E; Griesser, H

    2015-11-01

    Extensive numerical investigations are undertaken to analyze and compare, for the first time, the performance, techno-economy, and power consumption of three-level electrical Duobinary, optical Duobinary, and PAM-4 modulation formats as candidates for high-speed next-generation PONs supporting downstream 40 Gb/s per wavelength signal transmission over standard SMFs in C-band. Optimization of transceiver bandwidths are undertaken to show the feasibility of utilizing low-cost and band-limited components to support next-generation PON transmissions. The effect of electro-absorption modulator chirp is examined for electrical Duobinary and PAM-4. Electrical Duobinary and optical Duobinary are power-efficient schemes for smaller transmission distances of 10 km SMFs and optical Duobinary offers the best receiver sensitivity albeit with a relatively high transceiver cost. PAM-4 shows the best power budget and cost-efficiency for larger distances of around 20 km, although it consumes more power. Electrical Duobinary shows the best trade-off between performance, cost and power dissipation.

  17. In vivo optical modulation of neural signals using monolithically integrated two-dimensional neural probe arrays

    NASA Astrophysics Data System (ADS)

    Son, Yoojin; Jenny Lee, Hyunjoo; Kim, Jeongyeon; Shin, Hyogeun; Choi, Nakwon; Justin Lee, C.; Yoon, Eui-Sung; Yoon, Euisik; Wise, Kensall D.; Geun Kim, Tae; Cho, Il-Joo

    2015-10-01

    Integration of stimulation modalities (e.g. electrical, optical, and chemical) on a large array of neural probes can enable an investigation of important underlying mechanisms of brain disorders that is not possible through neural recordings alone. Furthermore, it is important to achieve this integration of multiple functionalities in a compact structure to utilize a large number of the mouse models. Here we present a successful optical modulation of in vivo neural signals of a transgenic mouse through our compact 2D MEMS neural array (optrodes). Using a novel fabrication method that embeds a lower cladding layer in a silicon substrate, we achieved a thin silicon 2D optrode array that is capable of delivering light to multiple sites using SU-8 as a waveguide core. Without additional modification to the microelectrodes, the measured impedance of the multiple microelectrodes was below 1 MΩ at 1 kHz. In addition, with a low background noise level (±25 μV), neural spikes from different individual neurons were recorded on each microelectrode. Lastly, we successfully used our optrodes to modulate the neural activity of a transgenic mouse through optical stimulation. These results demonstrate the functionality of the 2D optrode array and its potential as a next-generation tool for optogenetic applications.

  18. An FPGA-based High Speed Parallel Signal Processing System for Adaptive Optics Testbed

    NASA Astrophysics Data System (ADS)

    Kim, H.; Choi, Y.; Yang, Y.

    In this paper a state-of-the-art FPGA (Field Programmable Gate Array) based high speed parallel signal processing system (SPS) for adaptive optics (AO) testbed with 1 kHz wavefront error (WFE) correction frequency is reported. The AO system consists of Shack-Hartmann sensor (SHS) and deformable mirror (DM), tip-tilt sensor (TTS), tip-tilt mirror (TTM) and an FPGA-based high performance SPS to correct wavefront aberrations. The SHS is composed of 400 subapertures and the DM 277 actuators with Fried geometry, requiring high speed parallel computing capability SPS. In this study, the target WFE correction speed is 1 kHz; therefore, it requires massive parallel computing capabilities as well as strict hard real time constraints on measurements from sensors, matrix computation latency for correction algorithms, and output of control signals for actuators. In order to meet them, an FPGA based real-time SPS with parallel computing capabilities is proposed. In particular, the SPS is made up of a National Instrument's (NI's) real time computer and five FPGA boards based on state-of-the-art Xilinx Kintex 7 FPGA. Programming is done with NI's LabView environment, providing flexibility when applying different algorithms for WFE correction. It also facilitates faster programming and debugging environment as compared to conventional ones. One of the five FPGA's is assigned to measure TTS and calculate control signals for TTM, while the rest four are used to receive SHS signal, calculate slops for each subaperture and correction signal for DM. With this parallel processing capabilities of the SPS the overall closed-loop WFE correction speed of 1 kHz has been achieved. System requirements, architecture and implementation issues are described; furthermore, experimental results are also given.

  19. A molecular mechanism of optic nerve regeneration in fish: the retinoid signaling pathway.

    PubMed

    Kato, Satoru; Matsukawa, Toru; Koriyama, Yoshiki; Sugitani, Kayo; Ogai, Kazuhiro

    2013-11-01

    The fish optic nerve regeneration process takes more than 100 days after axotomy and comprises four stages: neurite sprouting (1-4 days), axonal elongation (5-30 days), synaptic refinement (35-80 days) and functional recovery (100-120 days). We screened genes specifically upregulated in each stage from axotomized fish retina. The mRNAs for heat shock protein 70 and insulin-like growth factor-1 rapidly increased in the retinal ganglion cells soon after axotomy and function as cell-survival factors. Purpurin mRNA rapidly and transiently increased in the photoreceptors and purpurin protein diffusely increased in all nuclear layers at 1-4 days after injury. The purpurin gene has an active retinol-binding site and a signal peptide. Purpurin with retinol functions as a sprouting factor for thin neurites. This neurite-sprouting effect was closely mimicked by retinoic acid and blocked by its inhibitor. We propose that purpurin works as a retinol transporter to supply retinoic acid to damaged RGCs which in turn activates target genes. We also searched for genes involved in the second stage of regeneration. The mRNA of retinoid-signaling molecules increased in retinal ganglion cells at 7-14 days after injury and tissue transglutaminase and neuronal nitric oxide synthase mRNAs, RA-target genes, increased in retinal ganglion cells at 10-30 days after injury. They function as factors for the outgrowth of thick, long neurites. Here we present a retinoid-signaling hypothesis to explain molecular events during the early stages of optic nerve regeneration in fish. PMID:23994437

  20. Experimental observation of optical differentiation and optical Hilbert transformation using a single SOI microdisk chip

    PubMed Central

    Yang, Ting; Dong, Jianji; Liu, Li; Liao, Shasha; Tan, Sisi; Shi, Lei; Gao, Dingshan; Zhang, Xinliang

    2014-01-01

    Optical differentiation and optical Hilbert transformation play important roles in communications, computing, information processing and signal analysis in optical domain offering huge bandwidth. Meanwhile, silicon-based photonic integrated circuit is one of the most promising candidates for all-optical signal processing due to its intrinsic advantages of low power consumption, compact footprint, ultra-high speed and compatibility with electronic integrated circuits. In this study, we analyze the interrelation between first-order optical differentiation and optical Hilbert transformation and then experimentally demonstrate a feasible integrated scheme which can simultaneously function as first-order optical differentiation and optical Hilbert transformation based on a single microdisk resonator. This finding may motivate the development of integrated optical signal processors. PMID:24492519

  1. Optical microsystem for analysis of diffuse reflectance and fluorescence signals applied to early gastrointestinal cancer detection.

    PubMed

    Pimenta, Sara; Castanheira, Elisabete M S; Minas, Graça

    2015-01-30

    The detection of cancer at its earliest stage is crucial in order to increase the probability of a successful treatment. Optical techniques, specifically diffuse reflectance and fluorescence, may considerably improve the ability to detect pre-cancerous lesions. These techniques have high sensitivity to some biomarkers present on the tissues, providing morphological and biochemical information of normal and diseased tissue. The development of a chip sized spectroscopy microsystem, based on these techniques, will greatly improve the early diagnosis of gastrointestinal cancers. The main innovation is the detection of the spectroscopic signals using only few, but representative, spectral bands allowing for miniaturization. This paper presents the mathematical models, its validation and analysis for retrieving data of the measured spectroscopic signals. These models were applied to a set of phantoms clearly representative of gastrointestinal tissues, leading to a more accurate diagnostic by a pathologist. Moreover, it was demonstrated that the models can use the reconstructed spectroscopic signals based only on its extraction on those specific spectral bands. As a result, the viability of the spectroscopy microsystem implementation was proved.

  2. Efficient Nanosecond Dual-Signal Optical Parametric Generator with a Periodically Phase Reversed PPMgLN

    NASA Astrophysics Data System (ADS)

    Ji, Feng; Li, Xi-Fu; Zhang, Bai-Gang; Zhang, Tie-Li; Wang, Peng; Xu, De-Gang; Yao, Jian-Quan

    2007-11-01

    We report an efficient nanosecond optical parametric generator (OPG) with a periodically-phase-reversed periodically poled MgO:LiNbO3(ppr-PPMgLN), which produces two pairs of signal and idler waves. The OPG is pumped by a 1.064 μm Q-switched Nd:YVO4 laser. When the repetition rate is set at 10 kHz, the maximum average total output power of 570 mW is achieved, including 410 mW of dual-signal radiations and 160 mW of dual-idler radiations. The total conversion efficiency is 32.5%. The tunable dual-signal wavelengths in the range of 1.474-1.518 μm and 1.490-1.539 μm and the dual-idler of 3.826-3.558 μm and 3.726-3.451 μm are obtained by changing the crystal temperature from 30°C to 200°C.

  3. Digital-signal-processor-based dynamic imaging system for optical tomography.

    PubMed

    Lasker, Joseph M; Masciotti, James M; Schoenecker, Matthew; Schmitz, Christoph H; Hielscher, Andreas H

    2007-08-01

    In this article, we introduce a dynamic optical tomography system that is, unlike currently available analog instrumentation, based on digital data acquisition and filtering techniques. At the core of this continuous wave instrument is a digital signal processor (DSP) that collects, collates, processes, and filters the digitized data set. The processor is also responsible for managing system timing and the imaging routines which can acquire real-time data at rates as high as 150 Hz. Many of the synchronously timed processes are controlled by a complex programmable logic device that is also used in conjunction with the DSP to orchestrate data flow. The operation of the system is implemented through a comprehensive graphical user interface designed with LABVIEW software which integrates automated calibration, data acquisition, data organization, and signal postprocessing. Performance analysis demonstrates very low system noise (approximately 1 pW rms noise equivalent power), excellent signal precision (<0.04%-0.2%) and long term system stability (<1% over 40 min). A large dynamic range (approximately 190 dB) accommodates a wide scope of measurement geometries and tissue types. First experiments on tissue phantoms show that dynamic behavior is accurately captured and spatial location can be correctly tracked using this system.

  4. Frequency domain photothermoacoustic signal amplitude dependence on the optical properties of water: turbid polyvinyl chloride-plastisol system

    SciTech Connect

    Spirou, Gloria M.; Mandelis, Andreas; Vitkin, I. Alex; Whelan, William M

    2008-05-10

    Photoacoustic (more precisely, photothermoacoustic) signals generated by the absorption of photons can be related to the incident laser fluence rate. The dependence of frequency domain photoacoustic (FD-PA) signals on the optical absorption coefficient ({mu}a) and the effective attenuation coefficient ({mu}eff) of a turbid medium [polyvinyl chloride-plastisol (PVCP)] with tissuelike optical properties was measured, and empirical relationships between these optical properties and the photoacoustic (PA) signal amplitude and the laser fluence rate were derived for the water (PVCP system with and without optical scatterers). The measured relationships between these sample optical properties and the PA signal amplitude were found to be linear, consistent with FD-PA theory: {mu}a=a(A/{phi})-b and {mu}eff=c(A/{phi})+d, where {phi} is the laser fluence, A is the FD-PA amplitude, and a,...,d are empirical coefficients determined from the experiment using linear frequency-swept modulation and a lock-in heterodyne detection technique. This quantitative technique can easily be used to measure the optical properties of general turbid media using FD-PAs.

  5. Sapphire-fiber-based intrinsic Fabry-Perot interferometer

    NASA Technical Reports Server (NTRS)

    Wang, Anbo; Gollapudi, Sridhar; Murphy, Kent A.; May, Russell G.; Claus, Richard O.

    1992-01-01

    A sapphire optical fiber intrinsic Fabry-Perot interferometric sensor is demonstrated. A length of multimode sapphire fiber that functions as a Fabry-Perot cavity is spliced to a silica single-mode fiber. The interferometric signals of this sensor are produced by the interference between the reflection from the silica-sapphire fiber splice and the reflection from the free end face of the sapphire fiber. This sensor has been demonstrated for temperature measurement. A resolution of 0.2 C has been obtained over a measurement range of 310 C to 976 C.

  6. Role of JAK/STAT signaling in neuroepithelial stem cell maintenance and proliferation in the Drosophila optic lobe

    SciTech Connect

    Wang, Wei; Li, Yonggang; Zhou, Liya; Yue, Haitao; Luo, Hong

    2011-07-15

    Highlights: {yields} JAK/STAT activity is graded in the Drosophila optic lobe neuroepithelium. {yields} Inactivation of JAK signaling causes disintegration of the optic lobe neuroepithelium and depletion of the neuroepithelial stem cells. {yields} JAK pathway overactivation promotes neuroepithelial overgrowth. {yields} Notch signaling acts downstream of JAK/STAT to promote neuroepithelial growth and expansion. -- Abstract: During Drosophila optic lobe development, proliferation and differentiation must be tightly modulated to reach its normal size for proper functioning. The JAK/STAT pathway plays pleiotropic roles in Drosophila development and in the larval brain, has been shown to inhibit medulla neuroblast formation. In this study, we find that JAK/STAT activity is required for the maintenance and proliferation of the neuroepithelial stem cells in the optic lobe. In loss-of-function JAK/STAT mutant brains, the neuroepithelial cells lose epithelial cell characters and differentiate prematurely while ectopic activation of this pathway is sufficient to induce neuroepithelial overgrowth in the optic lobe. We further show that Notch signaling acts downstream of JAK/STAT to control the maintenance and growth of the optic lobe neuroepithelium. Thus, in addition to its role in suppression of neuroblast formation, the JAK/STAT pathway is necessary and sufficient for optic lobe neuroepithelial growth.

  7. Full-Duplex Link Providing Alternative Wired and Wireless Broadband Access for the Wavelength-Division Multiplexing Passive Optical Network with a Uniform Converged Signal Format

    NASA Astrophysics Data System (ADS)

    Ma, Jianxin

    2014-05-01

    A full-duplex link implementing alternative wired and wireless access for wavelength-division multiplexing passive optical network is proposed with the uniformed three-tone converged optical signal, which provides a wired or wireless downlink access signal alternatively and an uplink optical carrier. The uplink optical carrier reversed by the converged optical signal makes the hybrid optical node unit free from the optical source. The simulation results show that the full-duplex link with a 10-Gb/s 16-quadrature amplitude modulation (16-QAM) downstream and 5 Gb/s binary upstream can provide both wired access with a bit-error rate below 10-9 and radio-over-fiber-based wireless access with a bit-error rate below 10-7 over 40 km of fiber without an optical source and optical amplifier in the hybrid optical node unit.

  8. All optical up-converted signal generation with high dispersion tolerance using frequency quadrupling technique for radio over fiber system

    NASA Astrophysics Data System (ADS)

    Gu, Yiying; Zhao, Jiayi; Hu, Jingjing; Kang, Zijian; Zhu, Wenwu; Fan, Feng; Han, Xiuyou; Zhao, Mingshan

    2016-05-01

    A novel all optical up-converted signal generation scheme with optical single-sideband (OSSB) technique for radio over fiber (RoF) application is presented and experimentally demonstrated using low-bandwidth devices. The OSSB signal is generated by one low-bandwidth intensity LiNbO3 Mach-Zehnder modulator (LN-MZM) under frequency quadrupling modulation scheme and one low-bandwidth LN-MZM under double sideband carrier suppressed modulation (DSB-CS) scheme. The proposed all OSSB generation scheme is capable of high tolerance of fiber chromatic dispersion induced power fading (DIPF) effect. Benefiting from this novel OSSB generation scheme, a 26 GHz radio frequency (RF) signal up-conversion is realized successfully when one sideband of the optical LO signal is reused as the optical carrier for intermediate frequency (IF) signal modulation. The received vector signal transmission over long distance single-mode fiber (SMF) shows negligible DIPF effect with the error vector magnitude (EVM) of 15.7% rms. In addition, a spurious free dynamic range (SFDR) of the OSSB up-converting system is measured up to 81 dB Hz2/3. The experiment results indicate that the proposed system may find potential applications in future wireless communication networks, especially in microcellular personal communication system (MPCS).

  9. All-optical AND gate with improved extinction ratio using signal induced nonlinearities in a bulk semiconductor optical amplifier.

    PubMed

    Guo, L Q; Connelly, M J

    2006-04-01

    An all-optical AND gate based on optically induced nonlinear polarization rotation of a probe light in a bulk semiconductor optical amplifier is realized at a bit rate of 2.5Gbit/s. By operating the AND gate in an up and inverted wavelength conversion scheme, the extinction ratio is improved by 8dB compared with previously published work.

  10. Influence of Color Noise on Processing of Optical Signals by Swarm Intellect Algorithm

    NASA Astrophysics Data System (ADS)

    Galimullin, D. Z.; Sibgatullin, M. E.; Kamalova, D. I.; Salakhov, M. Kh.

    2015-09-01

    An approach based on stochastic particle swarm optimization was applied for the mathematical processing of spectral profiles with noise. To test the algorithm's stability with respect to noise, we used the noises with different values of the Hurst index which characterizes noise component from the point of view of prevalence of low-frequency or high-frequency component in it. The Hurst index varied from 0.1 to 0.9. An influence of color noise with level from 1 to 10% on processing of optical signals by particle swarm optimization algorithm was analyzed. This method is shown to be stable with respect to noise with the level of 10% if it's the Hurst index does not exceed the value of 0.5.

  11. Fiber-optic transmission of microwave 8 phase-PSK signal

    NASA Astrophysics Data System (ADS)

    Way, W. I.; Gardina, M. F.

    1987-01-01

    Transmission of a 6 GHz, 8-phase phase-shift-keyed signal over a 12.5-km single-mode fiber in the 1.3 micron wavelength region, with a 5-dB power margin, was demonstrated for the first time. A digital modem operating at 26 Msymbol/s (78 Mbit/s), a high speed multimode InGaAsP laser diode, and a high speed PIN diode were used in this fiber-optic transmission system. Floor characteristics and a power penalty observed in the system bit error rate performance were found to be caused by the intensity noise of the laser diode, particularly the reflection-induced intensity noise.

  12. The influrence of an optical receiving system on statistical characteristics of a lidar signal

    NASA Technical Reports Server (NTRS)

    Zuev, A. E.; Banakh, V. A.; Mironov, V. L.

    1986-01-01

    The effects connected with correlation of direct and backward waves propagating through the same randomly inhomogeneous media can be observed along the path with refection in a turbulent atmosphere. In particular, the mean intensity of the reflected wave can increase in comparison with the wave propagating in the forward direction at a doubled distance; the intensity fluctuations can become stronger. These effects depend on the strength of optical turbulence, as well as on the diffraction sizes of the exit apertures of the source and the reflector. However, the focusing of radiation reflected with a receiving telescope leads, in some cases, to the fact that the dependence of amplification effects on the parameters becomes essentially different. This should be taken into account when alayzing the lidar signals. The effect of backscattering amplification and amplification of the intensity fluctuations is discussed.

  13. Motion compensation of optical mapping signals from isolated beating rat hearts

    NASA Astrophysics Data System (ADS)

    Stender, B.; Ernst, F.; Wang, B.; Zhang, Z. X.; Schlaefer, A.

    2013-09-01

    Optical mapping is a well established technique for recording monophasic action potential traces on the epicardial surface of isolated hearts. This measuring technique offers a high spatial resolution but it is sensitive towards myocardial motion. Motion artifacts occur because the mapping between a certain tissue portion sending out fluorescent light and a pixel of the photo detector changes over time. So far this problem has been addressed by suppressing the motion or ratiometric imaging. We developed a different approach to compensate the motion artifacts based on image registration. We could demonstrate how an image deformation field temporally changing with the heart motion could be determined. Using these deformation field time series for image transformation motion signals could be generated for each image pixel which were then successfully applied to remove baseline shift and compensate motion artifacts potentially leading to errors within maps of the first arrival time. The investigation was based on five different rat hearts stained with Di-4-ANEPPS.

  14. Optimal Signal Filtration in Optical Sensors with Natural Squeezing of Vacuum Noises

    NASA Technical Reports Server (NTRS)

    Gusev, A. V.; Kulagin, V. V.

    1996-01-01

    The structure of optimal receiver is discussed for optical sensor measuring a small displacement of probe mass. Due to nonlinear interaction of the field and the mirror, a reflected wave is in squeezed state (natural squeezing), two quadratures of which are correlated and therefore one can increase signal-to-noise ratio and overcome the SQL. A measurement procedure realizing such correlation processing of two quadratures is clarified. The required combination of quadratures can be produced via mixing of pump field reflected from the mirror with local oscillator phase modulated field in duel-detector homodyne scheme. Such measurement procedure could be useful not only for resonant bar gravitational detector but for laser longbase interferometric detectors as well.

  15. Metallic Nanoislands on Graphene as Highly Sensitive Transducers of Mechanical, Biological, and Optical Signals

    PubMed Central

    2016-01-01

    This article describes an effect based on the wetting transparency of graphene; the morphology of a metallic film (≤20 nm) when deposited on graphene by evaporation depends strongly on the identity of the substrate supporting the graphene. This control permits the formation of a range of geometries, such as tightly packed nanospheres, nanocrystals, and island-like formations with controllable gaps down to 3 nm. These graphene-supported structures can be transferred to any surface and function as ultrasensitive mechanical signal transducers with high sensitivity and range (at least 4 orders of magnitude of strain) for applications in structural health monitoring, electronic skin, measurement of the contractions of cardiomyocytes, and substrates for surface-enhanced Raman scattering (SERS, including on the tips of optical fibers). These composite films can thus be treated as a platform technology for multimodal sensing. Moreover, they are low profile, mechanically robust, semitransparent and have the potential for reproducible manufacturing over large areas. PMID:26765039

  16. Multi-level signaling in the Stokes space and its application to large-capacity optical communications.

    PubMed

    Kikuchi, Kazuro; Kawakami, Shojiro

    2014-04-01

    The Stokes vector of an optical signal does not depend on its absolute phase; therefore, we can construct the phase-insensitive optical communication system, using the Stokes vector as a modulation parameter. In such a system, multi-level optical signals can effectively be designed in the three-dimensional Stokes space and demodulated either by direct detection or by coherent detection, where low-complexity digital-signal processing (DSP) is employed. Although this system has the disadvantage that adaptive equalizers can hardly be implemented in the digital domain, it is still an attractive solution to large-capacity (≥ 100 Gbit/s) and medium-or short-reach (≤ 100 km) transmission. In this paper, we discuss the receiver configuration for the multi-level signal in the Stokes space and the efficient DSP algorithm for demodulating such a signal. Simulation results demonstrate that 2-, 4-, 8-, 16-, and 32-ary signals in the Stokes space have good bit-error rate (BER) characteristics. Especially, the 16-ary signal at the moderate symbol rate of 25 Gsymbol/s can reach the bit rate of 100 Gbit/s even by using direct detection.

  17. Hepatitis B virus inhibits intrinsic RIG-I and RIG-G immune signaling via inducing miR146a

    PubMed Central

    Hou, Zhaohua; Zhang, Jian; Han, Qiuju; Su, Chenhe; Qu, Jing; Xu, Dongqing; Zhang, Cai; Tian, Zhigang

    2016-01-01

    Previous studies showed that hepatitis B virus (HBV), as a latency invader, attenuated host anti-viral immune responses. miRNAs were shown to be involved in HBV infection and HBV-related diseases, however, the precise role of miRNAs in HBV-mediated immunosuppression remains unclear. Here, we observed that down-regulated RIG-I like receptors might be one critical mechanism of HBV-induced suppression of type I IFN transcription in both HBV+ hepatoma cell lines and liver cancer tissues. Then, miR146a was demonstrated to negatively regulate the expression of RIG-I-like receptors by directly targeting both RIG-I and RIG-G. Further investigation showed that antagonizing miR146a by anti-sense inhibitors or sponge approach accelerated HBV clearance and reduced HBV load both in vitro and in a HBV-carrying mouse model. Therefore, our findings indicated that HBV-induced miR146a attenuates cell-intrinsic anti-viral innate immunity through targeting RIG-I and RIG-G, and silencing miR146a might be an effective target to reverse HBV-induced immune suppression. PMID:27210312

  18. Optical design and illumination simulation of Fresnel lenses for marine signal lanterns

    NASA Astrophysics Data System (ADS)

    Park, Seung Nam; Park, Chul Woung; Kim, Yong Wan; Cho, Hyun Seok; Jo, Jae Heung; Kim, Jong Tae

    2005-02-01

    Providing marine signal lanterns, Fresnel lens has been adopted to transfer the beam from the lanterns up to 10 nautical miles (18.53 km). The Fresnel lens with diameters of 250 mm was designed by a ray tracing program and optimized by adjusting the groove parameters of the lens. Each optical sag element which is a part of a lens was independently designed by using the analytical method. The angular luminous intensity distributions (ALID) of this lens were calculated by the illumination analysis program considering the ALID of a light bulb. The ALID of a C-8 type bulb (24 W) was measured with a goniophotometer and its luminous flux was measured by an integrating sphere to be 397 lm. At the best alignment of the bulb, the maximum luminous intensity of the lantern was more than 1000 cd for the 250 mm lens. The ALID was investigated as a function of distance from the lens focus to determine the tolerance margin of the alignment. Horizontal deviations of the light bulb from the focus along the optical axis widened the angular FWHM of the vertical ALID. However, vertical deviations caused shifts of the vertical ALID without spreading the angular FWHM. The designed 250 mm aspherical lens of marine signal lantern was made by the injection molding with single peace acryl. We measured the luminous intensity distribution of acryl lens and found that the MLI of the lens was 827 cd. And the full width at half maximum of the diverging angle of the diverging beam was 3.5 deg. Although the measured MLI was 83% of the calculated result, it would be increased with surface polishing of prototype molding pattern.

  19. Geometric intrinsic symmetries

    SciTech Connect

    Gozdz, A. Szulerecka, A.; Pedrak, A.

    2013-08-15

    The problem of geometric symmetries in the intrinsic frame of a many-body system (nucleus) is considered. An importance of symmetrization group notion is discussed. Ageneral structure of the intrinsic symmetry group structure is determined.

  20. Eyecup scope—optical recordings of light stimulus-evoked fluorescence signals in the retina

    PubMed Central

    Hausselt, Susanne E.; Breuninger, Tobias; Castell, Xavier; Denk, Winfried; Margolis, David J.; Detwiler, Peter B.

    2009-01-01

    Dendritic signals play an essential role in processing visual information in the retina. To study them in neurites too small for electrical recording, we developed an instrument that combines a multi-photon (MP) microscope with a through-the-objective high-resolution visual stimulator. An upright microscope was designed that uses the objective lens for both MP imaging and delivery of visual stimuli to functionally intact retinal explants or eyecup preparations. The stimulator consists of a miniature liquid-crystal-on-silicon display coupled into the optical path of an infrared-excitation laser-scanning microscope. A pair of custom-made dichroic filters allows light from the excitation laser and three spectral bands (‘colors’) from the stimulator to reach the retina, leaving two intermediate bands for fluorescence imaging. Special optics allow displacement of the stimulator focus relative to the imaging focus. Spatially resolved changes in calcium-indicator fluorescence in response to visual stimuli were recorded in dendrites of different types of mammalian retinal neurons. PMID:19023590

  1. Modeling and signal processing of magneto-optic images for aviation applications

    NASA Astrophysics Data System (ADS)

    Ramuhalli, P.; Slade, James; Park, U.; Xuan, Liang; Udpa, L.

    2003-10-01

    Magneto-optic imaging (MOI) is a relatively new technology that produces analog images of magnetic flux leakage from surface and subsurface defects. An alternating current carrying foil serves as the excitation source and induces eddy currents in a conducting test specimen. Under normal conditions, the associated magnetic flux is tangential to specimen surface. Anomalies in the specimen result in generating a normal component of the magnetic flux density. The magneto-optic sensor produces a binary valued image of this anomalous magnetic field. The current system has two shortcomings. First, the presence of a textured background due to the domain structures in the sensor makes detection of third layer cracks and corrosion difficult. Second, the qualitative nature of the MO images does not provide a basis for making quantitative improvements to the MOI system. The availability of a theoretical model that can simulate the MOI system performance is extremely important for the optimization of the MOI sensor and hardware system. This paper presents a finite element model and its use in understanding the capabilities of the MOI system. In addition the paper also presents signal-processing methods for eliminating the background noise.

  2. Signal and response properties indicate an optoacoustic effect underlying the intra-cochlear laser-optical stimulation

    NASA Astrophysics Data System (ADS)

    Kallweit, Nicole; Baumhoff, Peter; Krueger, Alexander; Tinne, Nadine; Heisterkamp, Alexander; Kral, Andrej; Maier, Hannes; Ripken, Tammo

    2016-02-01

    Optical cochlea stimulation is under investigation as a potential alternative to conventional electric cochlea implants in treatment of sensorineural hearing loss. If direct optical stimulation of spiral ganglion neurons (SGNs) would be feasible, a smaller stimulation volume and, therefore, an improved frequency resolution could be achieved. However, it is unclear whether the mechanism of optical stimulation is based on direct neuronal stimulation or on optoacoustics. Animal studies on hearing vs. deafened guinea pigs already identified the optoacoustic effect as potential mechanism for intra-cochlear optical stimulation. In order to characterize the optoacoustic stimulus more thoroughly the acoustic signal along the beam path of a pulsed laser in water was quantified and compared to the neuronal response properties of hearing guinea pigs stimulated with the same laser parameters. Two pulsed laser systems were used for analyzing the influence of variable pulse duration, pulse energy, pulse peak power and absorption coefficient. Preliminary results of the experiments in water and in vivo suggesta similar dependency of response signals on the applied laser parameters: Both datasets show an onset and offset signal at the beginning and the end of the laser pulse. Further, the resulting signal amplitude depends on the pulse peak power as well as the temporal development of the applied laser pulse. The data indicates the maximum of the first derivative of power as the decisive factor. In conclusion our findings strengthen the hypothesis of optoacoustics as the underlying mechanism for optical stimulation of the cochlea.

  3. All-optical multi-channel wavelength conversion of Nyquist 16 QAM signal using a silicon waveguide.

    PubMed

    Long, Yun; Liu, Jun; Hu, Xiao; Wang, Andong; Zhou, Linjie; Zou, Kaiheng; Zhu, Yixiao; Zhang, Fan; Wang, Jian

    2015-12-01

    We experimentally demonstrate on-chip all-optical multi-channel wavelength conversion of Nyquist 16 ary quadrature amplitude modulation (16 QAM) signal in a silicon waveguide. The measured optical signal-to-noise ratio (OSNR) penalties of wavelength conversion are ∼2  dB. The observed constellations of converted idlers indicate favorable performance of silicon-waveguide-based multi-channel wavelength conversion. We also experimentally study and compare the phase-conjugated wavelength conversion by degenerate four-wave mixing (FWM) and transparent wavelength conversion by non-degenerate FWM in the silicon waveguide.

  4. High-repetition-rate dual-signal intracavity optical parametric generator based on periodically-phase-reversal PPMgLN

    NASA Astrophysics Data System (ADS)

    Ji, Feng; Yao, Jianquan; Zheng, Fanghua; Li, Enbang; Zhang, Tieli; Zhao, Pu; Wang, Peng; Zhang, Baigang

    2007-10-01

    A high-repetition-rate dual-signal intracavity optical parametric generator (IOPG) inside a diode-end-pumped acousto-optically (AO) Q-switched Nd:YVO4 laser is presented. The nonlinear material is a periodically-phase-reversal periodically poled MgO-doped lithium niobate (ppr-PPMgLN). At an incident diode pump power of 6.1 W and a Q-switch repetition rate of 20 kHz, an average dual-signal output power of 0.44 W is achieved. The dual-signal wavelengths of 1477 and 1491 nm are obtained at a crystal temperature of 40 °C. The measurements of the beam quality factor of 1.4 and the pulse duration of 77 ns show good spatial and temporal overlaps for the dual-signal radiation.

  5. Reactivation of IgG-switched memory B cells by BCR-intrinsic signal amplification promotes IgG antibody production

    PubMed Central

    Lutz, Johannes; Dittmann, Kai; Bösl, Michael R; Winkler, Thomas H; Wienands, Jürgen; Engels, Niklas

    2015-01-01

    Secondary antibody responses are marked by faster kinetics, improved antibody affinity and a switch from IgM to other immunoglobulin isotypes, most notably IgG, compared with primary responses. These changes protect from reinfection and represent the principle of most vaccination strategies. Yet, the molecular mechanisms that underlie B-cell memory responses are unclear. Here we show, by inactivating the immunoglobulin tail tyrosine (ITT) signalling motif of membrane-bound IgG1 in the mouse, that the ITT facilitates maintenance and reactivation of IgG-switched memory B cells in vivo. The ITT motif equips IgG-switched cells with enhanced BCR signalling capacity, which supports their competitiveness in secondary immune reactions and drives the formation of IgG-secreting plasma cells even in the absence of T-cell help. Our results demonstrate that ITT signalling promotes the vigorous production of IgG antibodies and thus provide a molecular basis for humoral immunological memory. PMID:26815242

  6. Characterization and mitigation of coherent-optical-transition-radiation signals from a compressed electron beam

    NASA Astrophysics Data System (ADS)

    Lumpkin, A. H.; Sereno, N. S.; Berg, W. J.; Borland, M.; Li, Y.; Pasky, S. J.

    2009-08-01

    The Advanced Photon Source (APS) injector complex includes an option for rf photocathode (PC) gun beam injection into the 450-MeV S-band linac. At the 150-MeV point, a four-dipole chicane was used to compress the micropulse bunch length from a few ps to sub-0.5 ps (FWHM). Noticeable enhancements of the optical transition radiation (OTR) signal sampled after the APS chicane were then observed as has been reported in the Linac Coherent Light Source (LCLS) injector commissioning. A far-infrared (FIR) coherent transition radiation detector and interferometer were used to monitor the bunch compression process and correlate the appearance of localized spikes of OTR signal (5 to 10 times brighter than adjacent areas) within the beam-image footprint. We have performed spectral-dependency measurements at 375 MeV with a series of bandpass filters centered in 50-nm increments from 400 to 700 nm and with an imaging spectrometer and observed a broadband enhancement in these spikes. Mitigation concepts of the observed coherent OTR, which exhibits an intensity enhancement in the red part of the visible spectrum as compared to incoherent OTR, are described.

  7. VEGF Signaling through Neuropilin 1 Guides Commissural Axon Crossing at the Optic Chiasm

    PubMed Central

    Erskine, Lynda; Reijntjes, Susan; Pratt, Thomas; Denti, Laura; Schwarz, Quenten; Vieira, Joaquim M.; Alakakone, Bennett; Shewan, Derryck; Ruhrberg, Christiana

    2011-01-01

    Summary During development, the axons of retinal ganglion cell (RGC) neurons must decide whether to cross or avoid the midline at the optic chiasm to project to targets on both sides of the brain. By combining genetic analyses with in vitro assays, we show that neuropilin 1 (NRP1) promotes contralateral RGC projection in mammals. Unexpectedly, the NRP1 ligand involved is not an axon guidance cue of the class 3 semaphorin family, but VEGF164, the neuropilin-binding isoform of the classical vascular growth factor VEGF-A. VEGF164 is expressed at the chiasm midline and is required for normal contralateral growth in vivo. In outgrowth and growth cone turning assays, VEGF164 acts directly on NRP1-expressing contralateral RGCs to provide growth-promoting and chemoattractive signals. These findings have identified a permissive midline signal for axons at the chiasm midline and provide in vivo evidence that VEGF-A is an essential axon guidance cue. PMID:21658587

  8. Characterization and mitigation of coherent optical transition radiation signal from a compressed electron beam.

    SciTech Connect

    Lumpkin, A. H.; Sereno, N. S.; Berg, W.; Borland, M.; Li, Y.; Pasky, S. )

    2009-01-01

    The Advanced Photon Source (APS) injector complex includes an option for rf photocathode (PC) gun beam injection into the 450-MeV S-band linac. At the 150-MeV point, a four-dipole chicane was used to compress the micropulse bunch length from a few ps to sub-0.5 ps (FWHM). Noticeable enhancements of the optical transition radiation (OTR) signal sampled after the APS chicane were then observed as has been reported in the Linac Coherent Light Source (LCLS) injector commissioning. A far-infrared (FIR) coherent transition radiation detector and interferometer were used to monitor the bunch compression process and correlate the appearance of localized spikes of OTR signal (5 to 10 times brighter than adjacent areas) within the beam-image footprint. We have performed spectral-dependency measurements at 375 MeV with a series of bandpass filters centered in 50-nm increments from 400 to 700 nm and with an imaging spectrometer and observed a broadband enhancement in these spikes. Mitigation concepts of the observed coherent OTR, which exhibits an intensity enhancement in the red part of the visible spectrum as compared to incoherent OTR, are described.

  9. Photonic vector signal generation employing a novel optical direct-detection in-phase/quadrature-phase upconversion.

    PubMed

    Jiang, Wen-Jr; Lin, Chun-Ting; Ho, Chung-hung; Wei, Chia-Chien; Shih, Po-Tsung; Chen, Jason Jyehong; Chi, Sien

    2010-12-01

    This work demonstrates the feasibility of the generation of an RF direct-detection vector signal using optical in-phase/quadrature-phase (I/Q) upconversion. The advantage of the proposed transmitter is that no electrical mixer is needed to generate the RF signal. Therefore, I/Q data of RF signals are processed at baseband at the transmitter, which is independent of the carrier frequency of the generated RF signal. A 10 Gb/s 16 quadrature amplitude modulation signal is experimentally demonstrated. Following transmission over a 50 km single-mode fiber, the power penalty is negligible. Moreover, I/Q imbalance of the proposed transmitter is studied and compensated by digital signal processing, which is both numerically and experimentally verified.

  10. Wavelength conversion for polarization multiplexing signal using four-wave mixing in semiconductor optical amplifier with reduced polarization crosstalk

    NASA Astrophysics Data System (ADS)

    Zhou, Hui; Chen, Ming; Wan, Qiuzhen; Zheng, Zhiwei

    2016-06-01

    We investigated wavelength conversion for polarization multiplexing signal based on four-wave mixing in a semiconductor optical amplifier. We found that the converted signals endured crosstalk among the pol-muxed channels. We also proposed and demonstrated a wavelength conversion scheme with polarization diversity technique. By utilizing the technique, the converted polarization multiplexing signal can be received without crosstalk. In addition, the performance of the proposed system is numerically analyzed with respect to the bit error rate of the converted signal, different frequency spacing between signal and pump and modulated data rate. The simulation results show that the proposed scheme may be a promising method to realize transparent wavelength conversion for polarization multiplexing signals.

  11. Analysis of detected signal performance in multi-tracks of optical disk memory using convex-shaped recording mark

    NASA Astrophysics Data System (ADS)

    Otani, Yousuke; Nitta, Kouichi; Matoba, Osamu

    2014-09-01

    The possibility of multi-valued optical disk memory by using a convex-shaped recording mark fabricated by two-photon absorption is investigated. We fabricate a numerical model for convex-shaped recording marks and evaluate the signal characteristics in terms of the signal-to-noise ratio (SNR). The numerical results show that 6 bit data can be realized when the acceptable average SNR is 2 and a length of the mark is 4T.

  12. Ca2+ signaling in arterioles and small arteries of conscious, restrained, optical biosensor mice

    PubMed Central

    Fairfax, Seth T.; Mauban, Joseph R. H.; Hao, Scarlett; Rizzo, Mark A.; Zhang, Jin; Wier, W. Gil

    2014-01-01

    Two-photon fluorescence microscopy and conscious, restrained optical biosensor mice were used to study smooth muscle Ca2+ signaling in ear arterioles. Conscious mice were used in order to preserve normal mean arterial blood pressure (MAP) and sympathetic nerve activity (SNA). ExMLCK mice, which express a genetically-encoded smooth muscle-specific FRET-based Ca2+ indicator, were equipped with blood pressure telemetry and immobilized for imaging. MAP was 101 ± 4 mmHg in conscious restrained mice, similar to the freely mobile state (107 ± 3 mmHg). Oscillatory vasomotion or irregular contractions were observed in most arterioles (71%), with the greatest oscillatory frequency observed at 0.25 s−1. In a typical arteriole with an average diameter of ~35 μm, oscillatory vasomotion of a 5–6 μm magnitude was accompanied by nearly uniform [Ca2+] oscillations from ~0.1 to 0.5 μM, with maximum [Ca2+] occurring immediately before the rapid decrease in diameter. Very rapid, spatially uniform “Ca2+ flashes” were also observed but not asynchronous propagating Ca2+ waves. In contrast, vasomotion and dynamic Ca2+ signals were rarely observed in ear arterioles of anesthetized exMLCK biosensor mice. Hexamethonium (30 μg/g BW, i.p.) caused a fall in MAP to 74 ± 4 mmHg, arteriolar vasodilation, and abolition of vasomotion and synchronous Ca2+ transients. Summary: MAP and heart rate (HR) were normal during high-resolution Ca2+ imaging of conscious, restrained mice. SNA induced continuous vasomotion and irregular vasoconstrictions via spatially uniform Ca2+ signaling within the arterial wall. FRET-based biosensor mice and two-photon imaging provided the first measurements of [Ca2+] in vascular smooth muscle cells in arterioles of conscious animals. PMID:25339912

  13. Low-loss, silicon integrated, aluminum nitride photonic circuits and their use for electro-optic signal processing.

    PubMed

    Xiong, Chi; Pernice, Wolfram H P; Tang, Hong X

    2012-07-11

    Photonic miniaturization requires seamless integration of linear and nonlinear optical components to achieve passive and active functions simultaneously. Among the available material systems, silicon photonics holds immense promise for optical signal processing and on-chip optical networks. However, silicon is limited to wavelengths above 1.1 μm and does not provide the desired lowest order optical nonlinearity for active signal processing. Here we report the integration of aluminum nitride (AlN) films on silicon substrates to bring active functionalities to chip-scale photonics. Using CMOS-compatible sputtered thin films we fabricate AlN-on-insulator waveguides that exhibit low propagation loss (0.6 dB/cm). Exploiting AlN's inherent Pockels effect we demonstrate electro-optic modulation up to 4.5 Gb/s with very low energy consumption (down to 10 fJ/bit). The ultrawide transparency window of AlN devices also enables high speed modulation at visible wavelengths. Our low cost, wideband, carrier-free photonic circuits hold promise for ultralow power and high-speed signal processing at the microprocessor chip level.

  14. Photonic DPASK/QAM signal generation at microwave/millimeter-wave band based on an electro-optic phase modulator.

    PubMed

    Zhang, Ye; Xu, Kun; Zhu, Ran; Li, Jianqiang; Wu, Jian; Hong, Xiaobin; Lin, Jintong

    2008-10-15

    We have proposed and experimentally demonstrated two novel photonic architectures to generate differential-phase amplitude-shift keying and circular quadrature amplitude modulation signals at microwave/millimeter-wave band based on an electro-optic phase modulator. In our proposed schemes, the electronic driven circuits were greatly simplified by employing the photonic vector modulation technique.

  15. Genome-Wide Prediction of Intrinsic Disorder; Sequence Alignment of Intrinsically Disordered Proteins

    ERIC Educational Resources Information Center

    Midic, Uros

    2012-01-01

    Intrinsic disorder (ID) is defined as a lack of stable tertiary and/or secondary structure under physiological conditions in vitro. Intrinsically disordered proteins (IDPs) are highly abundant in nature. IDPs possess a number of crucial biological functions, being involved in regulation, recognition, signaling and control, e.g. their functional…

  16. A modular, low-cost, digital signal processor-based lock-in card for measuring optical attenuation

    NASA Astrophysics Data System (ADS)

    Barragán, L. A.; Artigas, J. I.; Alonso, R.; Villuendas, F.

    2001-01-01

    A modular, low-cost, digital signal processor (DSP)-based lock-in card is described for measuring optical attenuation. By transferring the lock-in operation from the analog domain to the digital domain, the nonlinearities gain, and offset errors and drifts are virtually eliminated. The dual phase lock-in operation has been implemented on the low-cost DSP56002 evaluation module (DSP56002EVM) of Motorola that is widely used in audio signal processing. This evaluation board contains a 24 bit DSP56002 DSP and a stereo CD-quality audio codec that makes the board ideal for implementing signal processing algorithms. Due to the maximum sampling rate of the codec embedded on the DSP56002EVM, the frequencies of the processed signals must be below 20 kHz. This specification is enough for the most common applications in the field of optics, where low or very low frequency (<1 kHz) references are frequent. The software algorithm implementing the lock-in amplifier can be particularized by the user on the basis of the needed performances. The effects of finite word length in the digital filter implementation are analyzed. This analysis reveals that a 24 bit word length is not enough to ensure the filter stability and the required frequency response. To overcome this problem, the double precision multiply mode must be used. When the DSP56002 enters this mode, double precision 48 bit by 48 bit multiplication can be performed. The lock-in performance has been tested. The measured amplitude variations of the reference sine signal are about 0.003%, which do not affect the signal measurement. The lock-in behaves like a band-pass filter centered on the reference frequency whose bandwidth is related to the low-pass filter cutoff frequency. The measured frequency response shows that the lock-in performs as theoretically predicted. The DSP56002EVM can be used as a lock-in for electrical signals in stand-alone operation. Besides, we have designed a card that interconnects to the DSP56002EVM

  17. Nematicons and Their Electro-Optic Control: Light Localization and Signal Readdressing via Reorientation in Liquid Crystals

    PubMed Central

    Piccardi, Armando; Alberucci, Alessandro; Assanto, Gaetano

    2013-01-01

    Liquid crystals in the nematic phase exhibit substantial reorientation when the molecules are driven by electric fields of any frequencies. Exploiting such a response at optical frequencies, self-focusing supports transverse localization of light and the propagation of self-confined beams and waveguides, namely “nematicons”. Nematicons can guide other light signals and interact with inhomogeneities and other beams. Moreover, they can be effectively deviated by using the electro-optic response of the medium, leading to several strategies for voltage-controlled reconfiguration of light-induced guided-wave circuits and signal readdressing. Hereby, we outline the main features of nematicons and review the outstanding progress achieved in the last twelve years on beam self-trapping and electro-optic readdressing. PMID:24108367

  18. Graphene-assisted nonlinear optical device for four-wave mixing based tunable wavelength conversion of QPSK signal.

    PubMed

    Hu, Xiao; Zeng, Mengqi; Wang, Andong; Zhu, Long; Fu, Lei; Wang, Jian

    2015-10-01

    We fabricate a nonlinear optical device based on a fiber pigtail cross-section coated with a single-layer graphene grown by chemical vapor deposition (CVD) method. Using such graphene-assisted nonlinear optical device, we experimentally demonstrate tunable wavelength conversion of a 10 Gbaud quadrature phase-shift keying (QPSK) signal by exploiting degenerate four-wave mixing (FWM) progress in graphene. We study the conversion efficiency as functions of the pump power and pump wavelength and evaluate the bit-error rate (BER) performance. The observed optical signal-to-noise ratio (OSNR) penalties for tunable QPSK wavelength conversion are less than 2.2 dB at a BER of 1 × 10(-3).

  19. Signal enhancement in optical projection tomography via virtual high dynamic range imaging of single exposure

    NASA Astrophysics Data System (ADS)

    Yang, Yujie; Dong, Di; Shi, Liangliang; Wang, Jun; Yang, Xin; Tian, Jie

    2015-03-01

    Optical projection tomography (OPT) is a mesoscopic scale optical imaging technique for specimens between 1mm and 10mm. OPT has been proven to be immensely useful in a wide variety of biological applications, such as developmental biology and pathology, but its shortcomings in imaging specimens containing widely differing contrast elements are obvious. The longer exposure for high intensity tissues may lead to over saturation of other areas, whereas a relatively short exposure may cause similarity with surrounding background. In this paper, we propose an approach to make a trade-off between capturing weak signals and revealing more details for OPT imaging. This approach consists of three steps. Firstly, the specimens are merely scanned in 360 degrees above a normal exposure but non-overexposure to acquire the projection data. This reduces the photo bleaching and pre-registration computation compared with multiple different exposures in conventional high dynamic range (HDR) imaging method. Secondly, three virtual channels are produced for each projection image based on the histogram distribution to simulate the low, normal and high exposure images used in the traditional HDR technology in photography. Finally, each virtual channel is normalized to the full gray scale range and three channels are recombined into one image using weighting coefficients optimized by a standard eigen-decomposition method. After applying our approach on the projection data, filtered back projection (FBP) algorithm is carried out for 3-dimentional reconstruction. The neonatal wild-type mouse paw has been scanned to verify this approach. Results demonstrated the effectiveness of the proposed approach.

  20. Signal processing method based on group delay calculation for distributed Bragg wavelength shift in optical frequency domain reflectometry.

    PubMed

    Wada, Daichi; Igawa, Hirotaka; Murayama, Hideaki; Kasai, Tokio

    2014-03-24

    A signal processing method based on group delay calculations is introduced for distributed measurements of long-length fiber Bragg gratings (FBGs) based on optical frequency domain reflectometry (OFDR). Bragg wavelength shifts in interfered signals of OFDR are regarded as group delay. By calculating group delay, the distribution of Bragg wavelength shifts is obtained with high computational efficiency. We introduce weighted averaging process for noise reduction. This method required only 3.5% of signal processing time which was necessary for conventional equivalent signal processing based on short-time Fourier transform. The method also showed high sensitivity to experimental signals where non-uniform strain distributions existed in a long-length FBG.

  1. Moderate plasma treatment enhances the quality of optically detected magnetic resonance signals of nitrogen-vacancy centres in nanodiamonds

    NASA Astrophysics Data System (ADS)

    Sotoma, Shingo; Igarashi, Ryuji; Shirakawa, Masahiro

    2016-05-01

    We demonstrate that a moderate plasma treatment increases the quality of optically detected magnetic resonance (ODMR) signals from negatively charged nitrogen-vacancy centres in nanodiamonds (NDs). We measured the statistics of the ODMR spectra of 50-nm-size NDs before and after plasma treatment. We then evaluated each ODMR spectrum in terms of fluorescence and ODMR intensities, line width and signal-to-noise (SN) ratio. Our results showed that plasma treatment for more than 10 min contributes to higher-quality ODMR signals, i.e. signals that are brighter, stronger, sharper and have a higher SN ratio. We showed that such signal improvement is due to alteration of the surface chemical states of the NDs by the plasma treatment. Our study contributes to the advancement of biosensing applications using ODMR of NDs.

  2. Colorless WDM-PON based on a Fabry-Pérot laser diode and reflective semiconductor optical amplifiers for simultaneous transmission of bidirectional gigabit baseband signals and broadcasting signal.

    PubMed

    Pham, Thang Tien; Kim, Hyun-Seung; Won, Yong-Yuk; Han, Sang-Kook

    2009-09-14

    A novel WDM-PON system delivering bidirectional baseband data and broadcasting data is proposed and demonstrated. A subcarrier multiplexing signal is broadcasted to all users by modulating a broadband optical source based on a Fabry-Pérot laser diode. Reflective semiconductor optical amplifiers are used as colorless modulators for the baseband data at both optical line terminal and remote optical network units. Transmission performance including bit error rate of bidirectional gigabit data and error vector magnitude of broadcasting data of many optical channels is investigated. Additionally, the data rate for the broadcasting signal was improved by using an external modulator.

  3. Optical injection locking of monolithically integrated photonic source for generation of high purity signals above 100 GHz.

    PubMed

    Balakier, Katarzyna; Fice, Martyn J; van Dijk, Frederic; Kervella, Gael; Carpintero, Guillermo; Seeds, Alwyn J; Renaud, Cyril C

    2014-12-01

    A monolithically integrated photonic source for tuneable mm-wave signal generation has been fabricated. The source consists of 14 active components, i.e. semiconductor lasers, amplifiers and photodetectors, all integrated on a 3 mm(2) InP chip. Heterodyne signals in the range between 85 GHz and 120 GHz with up to -10 dBm output power have been successfully generated. By optically injection locking the integrated lasers to an external optical comb source, high-spectral-purity signals at frequencies >100 GHz have been generated, with phase noise spectral density below -90 dBc/Hz being achieved at offsets from the carrier greater than 10 kHz.

  4. Optical injection locking of monolithically integrated photonic source for generation of high purity signals above 100 GHz.

    PubMed

    Balakier, Katarzyna; Fice, Martyn J; van Dijk, Frederic; Kervella, Gael; Carpintero, Guillermo; Seeds, Alwyn J; Renaud, Cyril C

    2014-12-01

    A monolithically integrated photonic source for tuneable mm-wave signal generation has been fabricated. The source consists of 14 active components, i.e. semiconductor lasers, amplifiers and photodetectors, all integrated on a 3 mm(2) InP chip. Heterodyne signals in the range between 85 GHz and 120 GHz with up to -10 dBm output power have been successfully generated. By optically injection locking the integrated lasers to an external optical comb source, high-spectral-purity signals at frequencies >100 GHz have been generated, with phase noise spectral density below -90 dBc/Hz being achieved at offsets from the carrier greater than 10 kHz. PMID:25606875

  5. Amplification of the signal intensity of fluorescence-based fiber-optic biosensors using a Fabry-Perot resonator structure.

    PubMed

    Hsieh, Meng-Chang; Chiu, Yi-Hsin; Lin, Sheng-Fu; Chang, Jenq-Yang; Chang, Chia-Ou; Chiang, Huihua Kenny

    2015-01-01

    Fluorescent biosensors have been widely used in biomedical applications. To amplify the intensity of fluorescence signals, this study developed a novel structure for an evanescent wave fiber-optic biosensor by using a Fabry-Perot resonator structure. An excitation light was coupled into the optical fiber through a laser-drilled hole on the proximal end of the resonator. After entering the resonator, the excitation light was reflected back and forth inside the resonator, thereby amplifying the intensity of the light in the fiber. Subsequently, the light was used to excite the fluorescent molecules in the reactive region of the sensor. The experimental results showed that the biosensor signal was amplified eight-fold when the resonator reflector was formed using a 92% reflective coating. Furthermore, in a simulation, the biosensor signal could be amplified 20-fold by using a 99% reflector. PMID:25690548

  6. Retrievals of Thick Cloud Optical Depth from the Geoscience Laser Altimeter System (GLAS) by Calibration of Solar Background Signal

    NASA Technical Reports Server (NTRS)

    Yang, Yuekui; Marshak, Alexander; Chiu, J. Christine; Wiscombe, Warren J.; Palm, Stephen P.; Davis, Anthony B.; Spangenberg, Douglas A.; Nguyen, Louis; Spinhirne, James D.; Minnis, Patrick

    2008-01-01

    Laser beams emitted from the Geoscience Laser Altimeter System (GLAS), as well as other space-borne laser instruments, can only penetrate clouds to a limit of a few optical depths. As a result, only optical depths of thinner clouds (< about 3 for GLAS) are retrieved from the reflected lidar signal. This paper presents a comprehensive study of possible retrievals of optical depth of thick clouds using solar background light and treating GLAS as a solar radiometer. To do so we first calibrate the reflected solar radiation received by the photon-counting detectors of GLAS' 532 nm channel, which is the primary channel for atmospheric products. The solar background radiation is regarded as a noise to be subtracted in the retrieval process of the lidar products. However, once calibrated, it becomes a signal that can be used in studying the properties of optically thick clouds. In this paper, three calibration methods are presented: (I) calibration with coincident airborne and GLAS observations; (2) calibration with coincident Geostationary Operational Environmental Satellite (GOES) and GLAS observations of deep convective clouds; (3) calibration from the first principles using optical depth of thin water clouds over ocean retrieved by GLAS active remote sensing. Results from the three methods agree well with each other. Cloud optical depth (COD) is retrieved from the calibrated solar background signal using a one-channel retrieval. Comparison with COD retrieved from GOES during GLAS overpasses shows that the average difference between the two retrievals is 24%. As an example, the COD values retrieved from GLAS solar background are illustrated for a marine stratocumulus cloud field that is too thick to be penetrated by the GLAS laser. Based on this study, optical depths for thick clouds will be provided as a supplementary product to the existing operational GLAS cloud products in future GLAS data releases.

  7. Cell-intrinsic role for IFN-α–STAT1 signals in regulating murine Peyer patch plasmacytoid dendritic cells and conditioning an inflammatory response

    PubMed Central

    Li, Haiyan S.; Gelbard, Alexander; Martinez, Gustavo J.; Esashi, Eiji; Zhang, Huiyuan; Nguyen-Jackson, Hoainam; Liu, Yong-Jun; Overwijk, Willem W.

    2011-01-01

    Plasmacytoid dendritic cells (pDCs) reside in bone marrrow and lymphoid organs in homeostatic conditions and typically secrete abundant quantities of type I interferons (IFNs) on Toll-like receptor triggering. Recently, a pDC population was identified within Peyer patches (PPs) of the gut that is distinguished by its lack of IFN production; however, the relationship of PP pDCs to pDCs in other organs has been unclear. We report that PP pDCs are derived from common DC progenitors and accumulate in response to Fms-like tyrosine kinase 3 ligand, yet appear divergent in transcription factor profile and surface marker phenotype, including reduced E2-2 and CCR9 expression. Type I IFN signaling via STAT1 has a cell-autonomous role in accrual of PP pDCs in vivo. Moreover, IFN-α enhances pDC generation from DC progenitors by a STAT1-dependent mechanism. pDCs that have been developed in the presence of IFN-α resemble PP pDCs, produce inflammatory cytokines, stimulate Th17 cell generation, and fail to secrete IFN-α on Toll-like receptor engagement. These results indicate that IFN-α influences the development and function of pDCs by inducing emergence of an inflammatory (Th17-inducing) antigen-presenting subset, and simultaneously regulating accumulation of pDCs in the intestinal microenvironment. PMID:21828128

  8. The impact of relative intensity noise on the signal in multiple reference optical coherence tomography

    NASA Astrophysics Data System (ADS)

    Neuhaus, Kai; Subhash, Hrebesh; Alexandrov, Sergey; Dsouza, Roshan; Hogan, Josh; Wilson, Carol; Leahy, Martin; Slepneva, Svetlana; Huyet, Guillaume

    2016-03-01

    Multiple reference optical coherence tomography (MR-OCT) applies a unique low-cost solution to enhance the scanning depth of standard time domain OCT by inserting an partial mirror into the reference arm of the interferometric system. This novel approach achieves multiple reflections for different layers and depths of an sample with minimal effort of engineering and provides an excellent platform for low-cost OCT systems based on well understood production methods for micro-mechanical systems such as CD/DVD pick-up systems. The direct integration of a superluminescent light-emitting diode (SLED) is a preferable solution to reduce the form- factor of an MR-OCT system. Such direct integration exposes the light source to environmental conditions that can increase fluctuations in heat dissipation and vibrations and affect the noise characteristics of the output spectrum. This work describes the impact of relative intensity noise (RIN) on the quality of the interference signal of MR-OCT related to a variety of environmental conditions, such as temperature.

  9. Cortical dynamics of semantic processing during sentence comprehension: evidence from event-related optical signals.

    PubMed

    Huang, Jian; Wang, Suiping; Jia, Shiwei; Mo, Deyuan; Chen, Hsuan-Chih

    2013-01-01

    Using the event-related optical signal (EROS) technique, this study investigated the dynamics of semantic brain activation during sentence comprehension. Participants read sentences constituent-by-constituent and made a semantic judgment at the end of each sentence. The EROSs were recorded simultaneously with ERPs and time-locked to expected or unexpected sentence-final target words. The unexpected words evoked a larger N400 and a late positivity than the expected ones. Critically, the EROS results revealed activations first in the left posterior middle temporal gyrus (LpMTG) between 128 and 192 ms, then in the left anterior inferior frontal gyrus (LaIFG), the left middle frontal gyrus (LMFG), and the LpMTG in the N400 time window, and finally in the left posterior inferior frontal gyrus (LpIFG) between 832 and 864 ms. Also, expected words elicited greater activation than unexpected words in the left anterior temporal lobe (LATL) between 192 and 256 ms. These results suggest that the early lexical-semantic retrieval reflected by the LpMTG activation is followed by two different semantic integration processes: a relatively rapid and transient integration in the LATL and a relatively slow but enduring integration in the LaIFG/LMFG and the LpMTG. The late activation in the LpIFG, however, may reflect cognitive control.

  10. Extreme temperature robust optical sensor designs and fault-tolerant signal processing

    DOEpatents

    Riza, Nabeel Agha; Perez, Frank

    2012-01-17

    Silicon Carbide (SiC) probe designs for extreme temperature and pressure sensing uses a single crystal SiC optical chip encased in a sintered SiC material probe. The SiC chip may be protected for high temperature only use or exposed for both temperature and pressure sensing. Hybrid signal processing techniques allow fault-tolerant extreme temperature sensing. Wavelength peak-to-peak (or null-to-null) collective spectrum spread measurement to detect wavelength peak/null shift measurement forms a coarse-fine temperature measurement using broadband spectrum monitoring. The SiC probe frontend acts as a stable emissivity Black-body radiator and monitoring the shift in radiation spectrum enables a pyrometer. This application combines all-SiC pyrometry with thick SiC etalon laser interferometry within a free-spectral range to form a coarse-fine temperature measurement sensor. RF notch filtering techniques improve the sensitivity of the temperature measurement where fine spectral shift or spectrum measurements are needed to deduce temperature.

  11. Congenic mice provide in vivo evidence for a genetic locus that modulates intrinsic transforming growth factor β1-mediated signaling and bone acquisition.

    PubMed

    Mukherjee, Aditi; Larson, Emily A; Carlos, Amy S; Belknap, John K; Rotwein, Peter; Klein, Robert F

    2012-06-01

    Osteoporosis, the most common skeletal disorder, is characterized by low bone mineral density (BMD) and an increased risk of fragility fractures. BMD is the best clinical predictor of future osteoporotic fracture risk, but is a complex trait controlled by multiple environmental and genetic determinants with individually modest effects. Quantitative trait locus (QTL) mapping is a powerful method for identifying chromosomal regions encompassing genes involved in shaping complex phenotypes, such as BMD. Here we have applied QTL analysis to male and female genetically-heterogeneous F(2) mice derived from a cross between C57BL/6 and DBA/2 strains, and have identified 11 loci contributing to femoral BMD. Further analysis of a QTL on mouse chromosome 7 following the generation of reciprocal congenic strains has allowed us to determine that the high BMD trait, which tracks with the DBA/2 chromosome and exerts equivalent effects on male and female mice, is manifested by enhanced osteogenic differentiation of mesenchymal stem cells (MSCs) in vitro and by increased growth of metatarsal bones in short-term primary culture. An insertion/deletion DNA polymorphism in Ltbp4 exon 12 that causes the in-frame removal of 12 codons in the DBA/2-derived gene maps within 0.6 Mb of the marker most tightly linked to the QTL. LTBP4, one of four paralogous mouse proteins that modify the bioavailability of the transforming growth factor β (TGF-β) family of growth factors, is expressed in differentiating MSC-derived osteoblasts and in long bones, and reduced responsiveness to TGF-β1 is observed in MSCs of mice homozygous for the DBA/2 chromosome 7. Taken together, our results identify a potential genetic and biochemical relationship between decreased TGF-β1-mediated signaling and enhanced femoral BMD that may be regulated by a variant LTBP4 molecule.

  12. Transmission and full-band coherent detection of polarization-multiplexed all-optical Nyquist signals generated by Sinc-shaped Nyquist pulses.

    PubMed

    Zhang, Junwen; Yu, Jianjun; Chi, Nan

    2015-09-01

    All optical method is considered as a promising technique for high symbol rate Nyquist signal generation, which has attracted a lot of research interests for high spectral-efficiency and high-capacity optical communication system. In this paper, we extend our previous work and report the fully experimental demonstration of polarization-division multiplexed (PDM) all-optical Nyquist signal generation based on Sinc-shaped Nyquist pulse with advanced modulation formats, fiber-transmission and single-receiver full-band coherent detection. Using this scheme, we have successfully demonstrated the generation, fiber transmission and single-receiver full-band coherent detection of all-optical Nyquist PDM-QPSK and PDM-16QAM signals up to 125-GBaud. 1-Tb/s single-carrier PDM-16QAM signal generation and full-band coherent detection is realized, which shows the advantage and feasibility of the single-carrier all-optical Nyquist signals.

  13. Transmission and full-band coherent detection of polarization-multiplexed all-optical Nyquist signals generated by Sinc-shaped Nyquist pulses

    PubMed Central

    Zhang, Junwen; Yu, Jianjun; Chi, Nan

    2015-01-01

    All optical method is considered as a promising technique for high symbol rate Nyquist signal generation, which has attracted a lot of research interests for high spectral-efficiency and high-capacity optical communication system. In this paper, we extend our previous work and report the fully experimental demonstration of polarization-division multiplexed (PDM) all-optical Nyquist signal generation based on Sinc-shaped Nyquist pulse with advanced modulation formats, fiber-transmission and single-receiver full-band coherent detection. Using this scheme, we have successfully demonstrated the generation, fiber transmission and single-receiver full-band coherent detection of all-optical Nyquist PDM-QPSK and PDM-16QAM signals up to 125-GBaud. 1-Tb/s single-carrier PDM-16QAM signal generation and full-band coherent detection is realized, which shows the advantage and feasibility of the single-carrier all-optical Nyquist signals. PMID:26323238

  14. Analysis of behavior of focusing error signals generated by astigmatic method when a focused spot moves beyond the radius of a land-groove-type optical disk

    NASA Astrophysics Data System (ADS)

    Shinoda, Masahisa; Nakatani, Hidehiko; Nakai, Kenya; Ohmaki, Masayuki

    2015-09-01

    We theoretically calculate behaviors of focusing error signals generated by an astigmatic method in a land-groove-type optical disk. The focusing error signal from the land does not coincide with that from the groove. This behavior is enhanced when a focused spot of an optical pickup moves beyond the radius of the optical disk. A gain difference between the slope sensitivities of focusing error signals from the land and the groove is an important factor with respect to stable focusing servo control. In our calculation, the format of digital versatile disc-random access memory (DVD-RAM) is adopted as the land-groove-type optical disk model, and the dependences of the gain difference on various factors are investigated. The gain difference strongly depends on the optical intensity distribution of the laser beam in the optical pickup. The calculation method and results in this paper will be reflected in newly developed land-groove-type optical disks.

  15. Spatial routing of optical beams through time-domain spatial-spectral filtering

    NASA Astrophysics Data System (ADS)

    Babbitt, W. R.; Mossberg, T. W.

    1995-04-01

    We propose a novel new method of temporal-waveform-controlled high-speed passive spatial routing of optical beams. The method provides for the redirection of optical signals contained within a single input beam into output directions that are specified entirely by temporal information encoded on the waveform of each incident signal. The routing is effected by means of deflection from spectrally structured spatial gratings that may be optically programmed into materials with or without intrinsic frequency selectivity.

  16. Confirmation of intrinsic electron gap states at nonpolar GaN(1-100) surfaces combining photoelectron and surface optical spectroscopy

    SciTech Connect

    Himmerlich, M. Eisenhardt, A.; Shokhovets, S.; Krischok, S.; Räthel, J.; Speiser, E.; Neumann, M. D.; Navarro-Quezada, A.; Esser, N.

    2014-04-28

    The electronic structure of GaN(1–100) surfaces is investigated in-situ by photoelectron spectroscopy (PES) and reflection anisotropy spectroscopy (RAS). Occupied surface states 3.1 eV below the Fermi energy are observed by PES, accompanied by surface optical transitions found in RAS around 3.3 eV, i.e., below the bulk band gap. These results indicate that the GaN(1–100) surface band gap is smaller than the bulk one due to the existence of intra-gap states, in agreement with density functional theory calculations. Furthermore, the experiments demonstrate that RAS can be applied for optical surface studies of anisotropic crystals.

  17. Confirmation of intrinsic electron gap states at nonpolar GaN(1-100) surfaces combining photoelectron and surface optical spectroscopy

    NASA Astrophysics Data System (ADS)

    Himmerlich, M.; Eisenhardt, A.; Shokhovets, S.; Krischok, S.; Räthel, J.; Speiser, E.; Neumann, M. D.; Navarro-Quezada, A.; Esser, N.

    2014-04-01

    The electronic structure of GaN(1-100) surfaces is investigated in-situ by photoelectron spectroscopy (PES) and reflection anisotropy spectroscopy (RAS). Occupied surface states 3.1 eV below the Fermi energy are observed by PES, accompanied by surface optical transitions found in RAS around 3.3 eV, i.e., below the bulk band gap. These results indicate that the GaN(1-100) surface band gap is smaller than the bulk one due to the existence of intra-gap states, in agreement with density functional theory calculations. Furthermore, the experiments demonstrate that RAS can be applied for optical surface studies of anisotropic crystals.

  18. Maintenance of Glia in the Optic Lamina Is Mediated by EGFR Signaling by Photoreceptors in Adult Drosophila

    PubMed Central

    Lee, Yuan-Ming; Sun, Y. Henry

    2015-01-01

    The late onset of neurodegeneration in humans indicates that the survival and function of cells in the nervous system must be maintained throughout adulthood. In the optic lamina of the adult Drosophila, the photoreceptor axons are surrounded by multiple types of glia. We demonstrated that the adult photoreceptors actively contribute to glia maintenance in their target field within the optic lamina. This effect is dependent on the epidermal growth factor receptor (EGFR) ligands produced by the R1-6 photoreceptors and transported to the optic lamina to act on EGFR in the lamina glia. EGFR signaling is necessary and sufficient to act in a cell-autonomous manner in the lamina glia. Our results suggest that EGFR signaling is required for the trafficking of the autophagosome/endosome to the lysosome. The loss of EGFR signaling results in cell degeneration most likely because of the accumulation of autophagosomes. Our findings provide in vivo evidence for the role of adult neurons in the maintenance of glia and a novel role for EGFR signaling in the autophagic flux. PMID:25909451

  19. Full duplex fiber link for alternative wired and wireless access based on SSB optical millimeter-wave with 4-PAM signal

    NASA Astrophysics Data System (ADS)

    Ma, Jianxin; Zhang, Junjie

    2015-03-01

    A novel full-duplex fiber-wireless link based on single sideband (SSB) optical millimeter (mm)-wave with 10 Gbit/s 4-pulse amplitude modulation (PAM) signal is proposed to provide alternative wired and 40 GHz wireless accesses for the user terminals. The SSB optical mm-wave with 4-PAM signal consists of two tones: one bears the 4-PAM signal and the other is unmodulated with high power. After transmission over the fiber to the hybrid optical network unit (HONU), the SSB optical mm-wave signal can be decomposed by fiber Bragg gratings (FBGs) as the SSB optical mm-wave signal with reduced carrier-to-sideband ratio (the baseband 4-PAM optical signal) and the uplink optical carrier for the wireless (wired) access. This makes the HONU free from the laser source. For the uplink, since the wireless access signal is converted to the baseband by power detection, both the transmitter in the HONU and the receiver in optical line terminal (OLT) are co-shared for both wireless and wired accesses, which makes the full duplex link much simpler. In our scheme, the optical electrical field of the square-root increment level 4-PAM signal assures an equal level spacing receiving for both the downlink wired and wireless accesses. Since the downlink wireless signal is down-converted to the baseband by power detection, RF local oscillator is unnecessary. To confirm the feasibility of our proposed scheme, a simulation full duplex link with 40 GHz SSB optical mm-wave with 10 Gbit/s 4-PAM signal is built. The simulation results show that both down- and up-links for either wired or wireless access can keep good performance even if the link length of the SSMF is extended to 40 km.

  20. Optical absorption and intrinsic recombination in relaxed and strained InAs{sub 1–x}Sb{sub x} alloys for mid-wavelength infrared application

    SciTech Connect

    Wen, Hanqing; Bellotti, Enrico

    2015-11-30

    The intrinsic carrier recombination lifetime in relaxed and strained InAs{sub 1−x}Sb{sub x} alloys is investigated using the full-band Green's function theory. By computing the phonon-perturbed electron self-energy of the system, both direct and phonon-assisted indirect Auger and radiative processes are studied as functions of antimony molar fractions, lattice temperatures and applied in-plane biaxial strains. To improve the overall accuracy of the calculation, an empirical pseudopotential band structure for the alloy is also fitted to the measured band extrema and effective masses under different biaxial strains. A set of effective screened potentials valid for all the needed antimony fractions x and biaxial strains ϵ, therefore, is obtained and applied to the calculation. The results showed reduced total Auger recombination rates and enhanced radiative recombination rates in InAsSb alloys at room temperature when a compressive strain is applied. Furthermore, the study on the widely employed mid-wavelength infrared detector material, InAs{sub 0.91}Sb{sub 0.09}, strained by an InAs substrate, demonstrated that much longer minority carrier lifetime can be achieved compared to that in the lattice-matched situation when the lattice temperature is above 200 K.

  1. Intrinsically Radioactive [64Cu]CuInS/ZnS Quantum Dots for PET and Optical Imaging: Improved Radiochemical Stability and Controllable Cerenkov Luminescence

    PubMed Central

    2015-01-01

    Functionalized quantum dots (QDs) have been widely explored for multimodality bioimaging and proven to be versatile agents. Attaching positron-emitting radioisotopes onto QDs not only endows their positron emission tomography (PET) functionality, but also results in self-illuminating QDs, with no need for an external light source, by Cerenkov resonance energy transfer (CRET). Traditional chelation methods have been used to incorporate the radionuclide, but these methods are compromised by the potential for loss of radionuclide due to cleavage of the linker between particle and chelator, decomplexation of the metal, and possible altered pharmacokinetics of nanomaterials. Herein, we described a straightforward synthesis of intrinsically radioactive [64Cu]CuInS/ZnS QDs by directly incorporating 64Cu into CuInS/ZnS nanostructure with 64CuCl2 as synthesis precursor. The [64Cu]CuInS/ZnS QDs demonstrated excellent radiochemical stability with less than 3% free 64Cu detected even after exposure to serum containing EDTA (5 mM) for 24 h. PEGylation can be achieved in situ during synthesis, and the PEGylated radioactive QDs showed high tumor uptake (10.8% ID/g) in a U87MG mouse xenograft model. CRET efficiency was studied as a function of concentration and 64Cu radioactivity concentration. These [64Cu]CuInS/ZnS QDs were successfully applied as an efficient PET/self-illuminating luminescence in vivo imaging agents. PMID:25549258

  2. Nearly diffraction-limited signal generated by a lower beam-quality pump in an optical parametric oscillator.

    PubMed

    Pearl, Shaul; Ehrlich, Yosi; Fastig, Shlomo; Rosenwaks, Salman

    2003-02-20

    The beam quality of an optical parametric oscillator (OPO) within a singly resonant, confocal-positive branch unstable resonator is investigated. Resonator configurations have been found in which the beam quality of the outgoing signal exceeds the beam quality of the pump. Cavity magnification and pump-pulse duration are found to determine the signal beam quality. It is shown that signal M2 decreases with increasing pump-pulse duration for a given cavity magnification. In an experimental demonstration of a LiNbO3 OPO within an unstable resonator, pumped by a multitransversal mode beam, a signal beam with an almost-single transversal mode has been generated, whereas the multitransversal mode behavior of the pump beam is projected on the idler beam. PMID:12617222

  3. Intrinsic Analysis Training Manual.

    ERIC Educational Resources Information Center

    Gow, Doris T.

    This manual is for the training of linking agents between Education R&D and schools and for training teachers in the process of intrinsic analysis of curriculum materials. Intrinsic analysis means analysis of the instruction or process through examination of the materials, or artifacts, including teacher and student materials, developer's…

  4. Rod-like plasmonic nanoparticles as optical building blocks: how differences in particle shape and structural geometry influence optical signal

    SciTech Connect

    Stender, Anthony

    2013-01-01

    Gold nanoparticles, particularly those with an anisotropic shape, have become a popular optical probe for experiments involving work on the nanoscale. However, to carry out such delicate and intricate experiments, it is first necessary to understand the detailed behavior of individual nanoparticles. In this series of experiments, optical and electron microscopy were utilized for the characterization of individual nanoparticles and small assemblies of nanoparticles. In the first experiment, gold nanorods were investigated. Single, isolated nanorods exhibit two maxima of localized surface plasmon resonance (LSPR), which are associated with the two nanorod axes. Upon the physical rotation of a nanorod at one of its LSPR wavelengths under polarized illumination, the optical behavior varies in a sinusoidal fashion. A dimer of nanorods exhibits optical behavior quite similar to a nanorod, except the LSPR maxima are shifted and broader. Under differential interference contrast (DIC) microscopy, a pair of nanorods separated by a distance below the diffraction limit can be distinguished from a single nanorod due to its optical behavior upon rotation. Dark field microscopy is unable to distinguish the two geometries. For the second set of experiments, the optical behavior of single gold nanorods at non-plasmonic wavelengths was investigated. The same nanorod was rotated with respect to a polarized light source under DIC, dark field, and polarized light microscopy. DIC microscopy was found to produce diffraction pattern peaks at non-plasmonic wavelengths, which could be altered by adjusting the setting of the polarizer. In the third set of experiments, the optical behavior of a single gold dumbbell and several simple dumbbell geometries were investigated with microscopy and simulations. The single dumbbell displayed behavior quite similar to that of a nanorod, but dumbbells exhibit a shift in both LSPR wavebands. Moreover, the shape of dumbbell particles allows them to

  5. Unbalanced Michelson's interferometer as a fiber optic distributed sensor of external signals

    NASA Astrophysics Data System (ADS)

    Chojnacki, M.; Szustakowski, Mieczyslaw; Zyczkowski, Marek

    2001-08-01

    The subject of this work is a novel fiber optic distributed sensor system. The system uses a technique called multiplexed reflectometric interferometry to measure dynamic strain in a network of single mode optical fiber sensors. The sensor is constructed on unbalanced fiber optic Michelson's interferometer is activated by series of double pulse. The time interval between those pulses depends on the length of the section of sensor. Acousto-optical modulator acts as an optical frequency shifter. A change in a frequency of electrical pulses exciting the modulator result in a frequency shift in each generated wave packet.

  6. Radiance profile enhancement for solar occultation measurements. [correction for signal distortions from spaceborne electro-optical sensor

    NASA Technical Reports Server (NTRS)

    Gomberg, R. I.

    1981-01-01

    It is pointed out that distortions in received signals in solar occultation measurements made from space derive from the systematic motion of the electrooptical sensor. In a wide range of cases, the motion-related signal distortions brought about by the instrument's full modulation transfer function are as important as those from the optics. It is shown through simulation that existing techniques in the field of digital-image processing can be applied directly to solar occultation experiments to correct for these distortions. The resulting enhanced radiation profile is found to be much closer to the true radiance profile than is the sampled output of the instrument.

  7. Real-time monitoring of intracellular signal transduction in PC12 cells by non-adiabatic tapered optical fiber biosensor

    NASA Astrophysics Data System (ADS)

    Zibaii, M. I.; Latifi, H.; Asadollahi, A.; Noraeipoor, Z.; Dargahi, L.

    2014-05-01

    Real-time observation of intracellular process of signal transduction is very useful for biomedical and pharmaceutical applications as well as for basic research work of cell biology. For feasible and reagentless observation of intracellular alterations in real time, we examined the use of a nonadiabatic tapered optical fiber (NATOF) biosensor for monitoring of intracellular signal transduction that was mainly translocation of protein kinase C via refractive index change in PC12 cells adhered on tapered fiber sensor without any indicator reagent. PC12 cells were stimulated with KCl . Our results suggest that complex intracellular reactions could be real-time monitored and characterized by NATOF biosensor.

  8. Signal and depth enhancement for in vivo flow cytometer measurement of ear skin by optical clearing agents

    PubMed Central

    Ding, Yimin; Wang, Jing; Fan, Zhichao; Wei, Dan; Shi, Rui; Luo, Qingming; Zhu, Dan; Wei, Xunbin

    2013-01-01

    The in vivo flow cytometry (IVFC) has shown a great potential for detecting circulating tumor cells quantitatively in the bloodstream. However, the detection depth suffers from the strong light scattering of tissue. In this study, an innovative ear skin optical clearing agent (ESOCA) is employed to improve the signal quality of the IVFC. Our results show that compared with commonly used glycerol, topical application of ESOCA can enhance the transmittance of rat ear significantly in vivo. The labeled red blood cells can be detected by the IVFC with higher signal quality and greater detection depth. This study is very helpful for potential tumor metastasis studies by the IVFC in deep tissues. PMID:24298412

  9. Free induction decay MR signal measurements toward ultra-low field MRI with an optically pumped atomic magnetometer.

    PubMed

    Oida, Takenori; Kobayashi, Tetsuo

    2013-01-01

    Ultra-low field magnetic resonance imaging (ULF-MRI) has attracted attention because of its low running costs and minimum patient exposure. An optically pumped atomic magnetometer (OPAM) is a magnetic sensor with high sensitivity in the low frequency range, which does not require a cryogenic cooling system. In an effort to develop a ULF-MRI, we attempted to measure the free induction decay MR signals with an OPAM. We successfully detected the MR signals by combining an OPAM and a flux transformer, demonstrating the feasibility of the proposed system.

  10. Dynamic full field optical coherence tomography: subcellular metabolic contrast revealed in tissues by interferometric signals temporal analysis

    PubMed Central

    Apelian, Clement; Harms, Fabrice; Thouvenin, Olivier; Boccara, A. Claude

    2016-01-01

    We developed a new endogenous approach to reveal subcellular metabolic contrast in fresh ex vivo tissues taking advantage of the time dependence of the full field optical coherence tomography interferometric signals. This method reveals signals linked with local activity of the endogenous scattering elements which can reveal cells where other OCT-based techniques fail or need exogenous contrast agents. We benefit from the micrometric transverse resolution of full field OCT to image intracellular features. We used this time dependence to identify different dynamics at the millisecond scale on a wide range of organs in normal or pathological conditions. PMID:27446672

  11. Fiber-optic push-pull sensor systems

    NASA Technical Reports Server (NTRS)

    Gardner, David L.; Brown, David A.; Garrett, Steven L.

    1991-01-01

    Fiber-optic push-pull sensors are those which exploit the intrinsically differential nature of an interferometer with concommitant benefits in common-mode rejection of undesired effects. Several fiber-optic accelerometer and hydrophone designs are described. Additionally, the recent development at the Naval Postgraduate School of a passive low-cost interferometric signal demodulator permits the development of economical fiber-optic sensor systems.

  12. Monitoring of enamel lesion remineralization by optical coherence tomography: an alternative approach towards signal analysis

    NASA Astrophysics Data System (ADS)

    Sadr, Alireza; Mandurah, Mona; Nakashima, Syozi; Shimada, Yasushi; Kitasako, Yuichi; Tagami, Junji; Sumi, Yasunori

    Early detection, monitoring and remineralization repair of enamel lesions are top research priorities in the modern dentistry focusing on minimal intervention concept for caries management. We investigate the use of swept-source optical coherence tomography system (SS-OCT) without polarization-sensing at 1319 nm wavelength developed for clinical dentistry (Dental OCT System Prototype 2, Panasonic Healthcare Co., Ltd., Japan) in quantitative assessment of artificial enamel lesions and their remineralization. Bovine enamel blocks were subjected to demineralization to create subsurface lesions approximately 130 μm in depth over 2 weeks, and subjected to remineralization in solution containing bioavailable calcium and 1ppm fluoride at pH 6.5 for 2 weeks. Cross-sectional images of sound, demineralized and remineralized specimens were captured under hydrated conditions by the OCT. Finally, the specimens were cut into sections for nanoindentation to measure hardness through the lesion under 2mN load. Reflectivity had increased with demineralization. OCT images of lesions showed a boundary closely suggesting the lesion depth that gradually progressed with demineralization time. After remineralization, the boundary depth gradually decreased and nanoindentation showed over 60% average hardness recovery rate. A significant negative correlation was found between the slope power-law regression as a measure of attenuation and overall nanohardness for a range of data covering sound, demineralized and remineralized areas. In conclusion, OCT could provide clear images of early enamel lesion extent and signal attenuation could indicate its severity and recovery. Clinical data of natural lesions obtained using Dental OCT and analyzed by this approach will also be presented. Study supported by GCOE IRCMSTBD and NCGG.

  13. Transmissive grating-reflective mirror-based fiber optic accelerometer for stable signal acquisition in industrial applications

    NASA Astrophysics Data System (ADS)

    Lee, Yeon-Gwan; Kim, Dae-Hyun; Kim, Chun-Gon

    2012-05-01

    This paper discusses an applicable fiber-optic accelerometer composed of a transmissive grating panel, a reflection mirror, and two optical fibers with a separation of quarter grating pitch as transceivers that monitor the low-frequency accelerations of civil engineering structures. This sensor structure brings together the advantages of both a simple sensor structure, which leads to simplified cable design by 50% in comparison with the conventional transmission-type fiber optic accelerometer, and a stable reflected signals acquisition with repeatability in comparison to the researched grating-reflection type fiber optic accelerometer. The vibrating displacement and sinusoidal acceleration measured from the proposed fiber optic sensor demonstrated good agreement with those of a commercial laser displacement sensor and a MEMS accelerometer without electromagnetic interference. The developed fiber optic accelerometer can be used in frequency ranges below 4.0 Hz with a margin of error that is less than 5% and a high sensitivity of 5.06 rad/(m/s)2.

  14. 25 Gbit/s differential phase-shift-keying signal generation using directly modulated quantum-dot semiconductor optical amplifiers

    SciTech Connect

    Zeghuzi, A. Schmeckebier, H.; Stubenrauch, M.; Bimberg, D.; Meuer, C.; Schubert, C.; Bunge, C.-A.

    2015-05-25

    Error-free generation of 25-Gbit/s differential phase-shift keying (DPSK) signals via direct modulation of InAs quantum-dot (QD) based semiconductor optical amplifiers (SOAs) is experimentally demonstrated with an input power level of −5 dBm. The QD SOAs emit in the 1.3-μm wavelength range and provide a small-signal fiber-to-fiber gain of 8 dB. Furthermore, error-free DPSK modulation is achieved for constant optical input power levels from 3 dBm down to only −11 dBm for a bit rate of 20 Gbit/s. Direct phase modulation of QD SOAs via current changes is thus demonstrated to be much faster than direct gain modulation.

  15. Neovibsanin B increases extracellular matrix proteins in optic nerve head cells via activation of Smad signalling pathway.

    PubMed

    Wang, Zhen; Xu, Wei; Rong, Ao; Lin, Yan; Qiu, Xu-Ling; Qu, Shen; Lan, Xian-Hai

    2015-01-01

    The present study demonstrates the effect of neovibsanin B on the synthesis and deposition of ECM proteins and the signalling pathways used in optic nerve head (ONH) astrocytes and lamina cribrosa (LC) cells. For investigation of the signalling pathway used by neovibsanin B, ONH cells were treated with neovibsanin B. Western blot and immunostaining analyses were used to examine the phosphorylation of proteins involved in Smad and non-Smad signalling pathway. The results revealed that ONH cells on treatment with neovibsanin B showed enhanced synthesis of extracellular matrix (ECM) proteins. Neovibsanin B induced phosphorylation of canonical signalling proteins, Smad2/3. However phosphorylation of non-canonical signalling proteins, extracellular signal-regulated kinases, p38, and c-Jun N-terminal kinases (JNK) 1/2 remained unaffected. There was also increase in co-localization of pSmad2/3 with Co-Smad4 in the nucleus of ONH astrocytes and LC cells indicating activation of the canonical Smad signalling pathway. Treatment of ONH cells with SIS3, inhibitor of Smad3 phosphorylation reversed the neovibsanin B stimulated ECM expression as well as activation of canonical pathway signalling molecules. In addition, inhibition of Smad2 or Smad3 using small interfering RNA (siRNA) also suppressed neovibsanin B stimulated ECM protein synthesis in ONH astrocytes and LC cells. Thus neovibsanin B utilizes the canonical Smad signalling pathway to stimulate ECM synthesis in human ONH cells. The neovibsanin B induced ECM synthesis and activation of the canonical Smad signalling pathway may be due to its effect on transforming growth factor-β2 (TGF-β2). However, further studies are under process to understand the mechanism.

  16. Monobenzyltin Complex C1 Induces Apoptosis in MCF-7 Breast Cancer Cells through the Intrinsic Signaling Pathway and through the Targeting of MCF-7-Derived Breast Cancer Stem Cells via the Wnt/β-Catenin Signaling Pathway.

    PubMed

    Fani, Somayeh; Dehghan, Firouzeh; Karimian, Hamed; Mun Lo, Kong; Ebrahimi Nigjeh, Siyamak; Swee Keong, Yeap; Soori, Rahman; May Chow, Kit; Kamalidehghan, Behnam; Mohd Ali, Hapipah; Mohd Hashim, Najihah

    2016-01-01

    Monobenzyltin Schiff base complex, [N-(3,5-dichloro-2-oxidobenzylidene)-4-chlorobenzyhydrazidato](o-methylbenzyl)aquatin(IV) chloride, C1, is an organotin non-platinum metal-based agent. The present study was conducted to investigate its effects on MCF-7 cells with respect to the induction of apoptosis and its inhibitory effect against MCF-7 breast cancer stem cells. As determined in a previous study, compound C1 revealed strong antiproliferative activity on MCF-7 cells with an IC50 value of 2.5 μg/mL. Annexin V/propidium iodide staining coupled with flow cytometry indicated the induction of apoptosis in treated cells. Compound C1 induced apoptosis in MCF-7 cells and was mediated through the intrinsic pathway with a reduction in mitochondrial membrane potential and mitochondrial cytochrome c release to cytosol. Complex C1 activated caspase 9 as a result of cytochrome c release. Subsequently, western blot and real time PCR revealed a significant increase in Bax and Bad expression and a significant decrease in the expression levels of Bcl2 and HSP70. Furthermore, a flow cytometric analysis showed that treatment with compound C1 caused a significant arrest of MCF-7 cells in G0/G1 phase. The inhibitory analysis of compound C1 against derived MCF-7 stem cells showed a significant reduction in the aldehyde dehydrogenase-positive cell population and a significant reduction in the population of MCF-7 cancer stem cells in primary, secondary, and tertiary mammospheres. Moreover, treatment with C1 down-regulated the Wnt/β-catenin self-renewal pathway. These findings indicate that complex C1 is a suppressive agent of MCF-7 cells that functions through the induction of apoptosis, cell cycle arrest, and the targeting of MCF-7-derived cancer stem cells. This work may lead to a better treatment strategy for the reduction of breast cancer recurrence. PMID:27529753

  17. Monobenzyltin Complex C1 Induces Apoptosis in MCF-7 Breast Cancer Cells through the Intrinsic Signaling Pathway and through the Targeting of MCF-7-Derived Breast Cancer Stem Cells via the Wnt/β-Catenin Signaling Pathway

    PubMed Central

    Fani, Somayeh; Dehghan, Firouzeh; Karimian, Hamed; Mun Lo, Kong; Ebrahimi Nigjeh, Siyamak; Swee Keong, Yeap; Soori, Rahman; May Chow, Kit; Kamalidehghan, Behnam; Mohd Ali, Hapipah; Mohd Hashim, Najihah

    2016-01-01

    Monobenzyltin Schiff base complex, [N-(3,5-dichloro-2-oxidobenzylidene)-4-chlorobenzyhydrazidato](o-methylbenzyl)aquatin(IV) chloride, C1, is an organotin non-platinum metal-based agent. The present study was conducted to investigate its effects on MCF-7 cells with respect to the induction of apoptosis and its inhibitory effect against MCF-7 breast cancer stem cells. As determined in a previous study, compound C1 revealed strong antiproliferative activity on MCF-7 cells with an IC50 value of 2.5 μg/mL. Annexin V/propidium iodide staining coupled with flow cytometry indicated the induction of apoptosis in treated cells. Compound C1 induced apoptosis in MCF-7 cells and was mediated through the intrinsic pathway with a reduction in mitochondrial membrane potential and mitochondrial cytochrome c release to cytosol. Complex C1 activated caspase 9 as a result of cytochrome c release. Subsequently, western blot and real time PCR revealed a significant increase in Bax and Bad expression and a significant decrease in the expression levels of Bcl2 and HSP70. Furthermore, a flow cytometric analysis showed that treatment with compound C1 caused a significant arrest of MCF-7 cells in G0/G1 phase. The inhibitory analysis of compound C1 against derived MCF-7 stem cells showed a significant reduction in the aldehyde dehydrogenase-positive cell population and a significant reduction in the population of MCF-7 cancer stem cells in primary, secondary, and tertiary mammospheres. Moreover, treatment with C1 down-regulated the Wnt/β-catenin self-renewal pathway. These findings indicate that complex C1 is a suppressive agent of MCF-7 cells that functions through the induction of apoptosis, cell cycle arrest, and the targeting of MCF-7-derived cancer stem cells. This work may lead to a better treatment strategy for the reduction of breast cancer recurrence. PMID:27529753

  18. Compact optical displacement sensing by detection of microwave signals generated from a monolithic passively mode-locked laser under feedback

    NASA Astrophysics Data System (ADS)

    Simos, Christos; Simos, Hercules; Nikas, Thomas; Syvridis, Dimitris

    2015-05-01

    A monolithic passively mode-locked laser is proposed as a compact optical sensor for displacements and vibrations of a reflecting object. The sensing principle relies on the change of the laser repetition frequency that is induced by optical feedback from the object under measurement. It has been previously observed that, when a semiconductor passively mode locked laser receives a sufficient level of optical feedback from an external reflecting surface it exhibits a repetition frequency that is no more determined by the mode-locking rule of the free-running operation but is imposed by the length of the external cavity. Therefore measurement of the resulting laser repetition frequency under self-injection permits the accurate and straightforward determination of the relative position of the reflecting object. The system has an inherent wireless capability since the repetition rate of the laser can be wirelessly detected by means of a simple antenna which captures the microwave signal generated by the saturable absorber and is emitted through the wiring of the laser. The sensor setup is very simple as it requires few optical components besides the laser itself. Furthermore, the deduction of the relative position of the reflecting object is straightforward and does not require any processing of the detected signal. The proposed sensor has a theoretical sub-wavelength resolution and its performance depends on the RF linewidth of the laser and the resolution of the repetition frequency measurement. Other physical parameters that induce phase changes of the external cavity could also be quantified.

  19. A new beat interference cancellation receiver with 3×3 optical coupler for the SSB-OOFDM signal with reduced guard band

    NASA Astrophysics Data System (ADS)

    Zhang, Ying; Ma, Jianxin

    2016-05-01

    In this paper, we present a new method to implement beat interference cancellation receiver (BICR) for single-sideband optical orthogonal frequency division multiplexing (SSB-OOFDM) signal, which improves the spectral efficiency (SE) by reducing the guard band (GB) between the optical carrier and the OOFDM signal while mitigating the impact of signal-signal beat interference (SSBI). This BICR structure is relatively simple using only an optical interleaver (IL), a 3×3 optical coupler (OC) and three photodiodes (PDs). A system simulation for the 40 Gbit/s 16-QAM SSB-OOFDM signal with the reduced GB is carried out to prove the feasibility of this new scheme. The simulation results show that our proposed BICR has a better performance to suppress SSBI and a higher SE than the conventional direct-detection receiver (CDDR) according to the EVMs and the constellations.

  20. Rare-earth-doped materials with application to optical signal processing, quantum information science, and medical imaging technology

    NASA Astrophysics Data System (ADS)

    Cone, R. L.; Thiel, C. W.; Sun, Y.; Böttger, Thomas; Macfarlane, R. M.

    2012-02-01

    Unique spectroscopic properties of isolated rare earth ions in solids offer optical linewidths rivaling those of trapped single atoms and enable a variety of recent applications. We design rare-earth-doped crystals, ceramics, and fibers with persistent or transient "spectral hole" recording properties for applications including high-bandwidth optical signal processing where light and our solids replace the high-bandwidth portion of the electronics; quantum cryptography and information science including the goal of storage and recall of single photons; and medical imaging technology for the 700-900 nm therapeutic window. Ease of optically manipulating rare-earth ions in solids enables capturing complex spectral information in 105 to 108 frequency bins. Combining spatial holography and spectral hole burning provides a capability for processing high-bandwidth RF and optical signals with sub-MHz spectral resolution and bandwidths of tens to hundreds of GHz for applications including range-Doppler radar and high bandwidth RF spectral analysis. Simply stated, one can think of these crystals as holographic recording media capable of distinguishing up to 108 different colors. Ultra-narrow spectral holes also serve as a vibration-insensitive sub-kHz frequency reference for laser frequency stabilization to a part in 1013 over tens of milliseconds. The unusual properties and applications of spectral hole burning of rare earth ions in optical materials are reviewed. Experimental results on the promising Tm3+:LiNbO3 material system are presented and discussed for medical imaging applications. Finally, a new application of these materials as dynamic optical filters for laser noise suppression is discussed along with experimental demonstrations and theoretical modeling of the process.