[Atmospheric non-methane hydrocarbons near plants of crude oil first treatment].

PubMed

Bustaffa, Elisa; De Marinis Loiotile, Annamaria; Farella, Genoveffa; Petraccone, Stefania; De Gennaro, Gianluigi; Bianchi, Fabrizio

2016-01-01

La continua espansione delle attività di perforazione ai fini dell'estrazione petrolifera in prossimità di aree abitate ha fatto sì che negli ultimi anni l'attenzione si focalizzasse sull'impatto di questo processo fortemente industrializzato sulla salute pubblica. Le comunità che vivono nei dintorni di impianti di questo tipo devono, infatti, fronteggiare diversi problemi, quali l'inquinamento atmosferico e acustico, la contaminazione del suolo e delle acque sotterranee, il traffico dei camion da e verso il sito, incidenti e malfunzionamenti all'interno dell'impianto. In questo contesto, la valutazione del rischio per la salute è ostacolata dal fatto che l'esposizione alle sostanze chimiche presenti non può essere valutata in via definitiva, poiché non sempre si è a conoscenza di tutti i composti immessi nell'ambiente né delle loro concentrazioni, per non parlare del problema delle coesposizioni ad altri inquinanti. Nonostante l'oramai conclamato e vasto interesse generato da questo argomento, ad oggi esistono pochi studi basati su popolazioni riguardanti gli effetti sulla salute delle comunità che vivono in prossimità dei siti di perforazione ed estrazione; ciò genera la necessità di condurre campagne di monitoraggio mirate e studi epidemiologici che verifichino l'eventuale esistenza e natura di pattern di malattie associati a tali attività. La presente rassegna bibliografica individua, quindi, i principali inquinanti atmosferici presenti in prossimità di un impianto di primo trattamento del greggio e cerca di fornire un quadro generale delle loro potenziali sorgenti e caratteristiche.

Nonevaporable getter coating chambers for extreme high vacuum

DOE PAGES

Stutzman, Marcy L.; Adderley, Philip A.; Mamun, Md Abdullah Al; ...

2018-03-01

Techniques for NEG coating a large diameter chamber are presented along with vacuum measurements in the chamber using several pumping configurations, with base pressure as low as 1.56x10^-12 Torr (N2 equivalent) with only a NEG coating and small ion pump. We then describe modifications to the NEG coating process to coat complex geometry chambers for ultra-cold atom trap experiments. Surface analysis of NEG coated samples are used to measure composition and morphology of the thin films. Finally, pressure measurements are compared for two NEG coated polarized electron source chambers: the 130 kV polarized electron source at Jefferson Lab and themore » upgraded 350 kV polarized 2 electron source, both of which are approaching or within the extreme high vacuum (XHV) range, defined as P<7.5x10^-13 Torr.« less

Nonevaporable getter coating chambers for extreme high vacuum

DOE Office of Scientific and Technical Information (OSTI.GOV)

Stutzman, Marcy L.; Adderley, Philip A.; Mamun, Md Abdullah Al

Techniques for NEG coating a large diameter chamber are presented along with vacuum measurements in the chamber using several pumping configurations, with base pressure as low as 1.56x10^-12 Torr (N2 equivalent) with only a NEG coating and small ion pump. We then describe modifications to the NEG coating process to coat complex geometry chambers for ultra-cold atom trap experiments. Surface analysis of NEG coated samples are used to measure composition and morphology of the thin films. Finally, pressure measurements are compared for two NEG coated polarized electron source chambers: the 130 kV polarized electron source at Jefferson Lab and themore » upgraded 350 kV polarized 2 electron source, both of which are approaching or within the extreme high vacuum (XHV) range, defined as P<7.5x10^-13 Torr.« less

The early expansion of anergic NKG2Apos/CD56dim/CD16neg natural killer cells represents a therapeutic target in haploidentical haematopoietic stem cell transplantation.

PubMed

Roberto, Alessandra; Di Vito, Clara; Zaghi, Elisa; Mazza, Emilia Maria Cristina; Capucetti, Arianna; Calvi, Michela; Tentorio, Paolo; Zanon, Veronica; Sarina, Barbara; Mariotti, Jacopo; Bramanti, Stefania; Tenedini, Elena; Tagliafico, Enrico; Bicciato, Silvio; Santoro, Armando; Roederer, Mario; Marcenaro, Emanuela; Castagna, Luca; Lugli, Enrico; Mavilio, Domenico

2018-04-26

Natural Killer cells are the first lymphocyte population to reconstitute early after non myelo-ablative and T cell-replete haploidentical hematopoietic stem cell transplantation with post-transplant infusion of cyclophosphamide. The present study characterizes the transient and predominant expansion starting from the 2nd week after haploidentical hematopoietic stem cell transplantation of a donor-derived unconventional subset of NKp46neg-low/CD56dim/CD16neg natural killer cells expressing remarkable high levels of CD94/NKG2A. Both transcription and phenotypic profiles indicated that unconventional NKp46neg-low/CD56dim/CD16neg natural killer cells are a distinct natural killer cell subpopulation with features of late stage differentiation, yet retaining proliferative capability and functional plasticity to generate conventional NKp46pos/CD56bright/CD16pos natural killer cells in response to interleukin-15 plus interleukin-18. While present at low frequency in healthy donors, unconventional NKp46neg-low/CD56dim/CD16neg natural killer cells are greatly expanded in the following 7 weeks after haploidentical hematopoietic stem cell transplantation and express high levels of the activating receptors NKGD and NKp30 as well as of the lytic granules Granzyme-B and Perforin. Nonetheless, NKp46neg-low/CD56dim/CD16neg natural killer cells displayed a markedly defective cytotoxicity that could be reversed by blocking the inhibitory receptor CD94/NKG2A. These data open new important perspectives to better understand the ontogenesis/homeostasis of human natural killer cells and to develop a novel immune-therapeutic approach that targets the inhibitory NKG2A check point, thus unleashing natural killer cell alloreactivity early after haploidentical hematopoietic stem cell transplantation. Copyright © 2018, Ferrata Storti Foundation.

Phenotypic and functional characteristics of CD4+ CD39+ FOXP3+ and CD4+ CD39+ FOXP3neg T-cell subsets in cancer patients.

PubMed

Schuler, Patrick J; Schilling, Bastian; Harasymczuk, Malgorzata; Hoffmann, Thomas K; Johnson, Jonas; Lang, Stephan; Whiteside, Theresa L

2012-07-01

Human CD4(+) CD39(+) regulatory T (Treg) cells hydrolyze exogenous adenosine triphosphate (ATP) and participate in immunosuppressive adenosine production. They contain two T-cell subsets whose role in mediating suppression is not understood. Frequencies of both CD4(+) CD39(+) subsets were evaluated in peripheral blood lymphocytes of 57 cancer patients and in tumor infiltrating lymphocytes (TILs) of 6 patients. CD4(+) CD39(+) and CD4(+) CD39(neg) T cells isolated using immunobeads and cell sorting were cultured under various conditions. Their conversion into CD39(+) FOXP3(+) CD25(+) or CD39(+) FOX(neg) CD25(neg) cells was monitored by multiparameter flow cytometry. Hydrolysis of exogenous ATP was measured in luminescence assays. Two CD4(+) CD39(+) cell subsets differing in expression of CD25, FOXP3, CTLA-4, CD121a, PD-1, latency associated peptide (LAP), glycoprotein A repetitions predominant (GARP), and the cytokine profile accumulated with equal frequencies in the blood and tumor tissues of cancer patients. The frequency of both subsets was significantly increased in cancer. CD39 expression levels correlated with the subsets' ability to hydrolyze ATP. Conventional CD4(+) CD39(neg) T cells incubated with IL-2 + TGF-β expanded to generate CD4(+) CD39(+) FOXP3(+) Treg cells, while CD4(+) CD39(+) FOXP3(neg) CD25(neg) subset cells stimulated via the TCR and IL-2 converted to FOXP3(+) CTLA4(+) CD25(+) TGF-β-expressing Treg cells. Among CD4(+) CD39(+) Treg cells, the CD4(+) CD39(+) FOXP3(neg) CD25(neg) subset serves as a reservoir of cells able to convert to Treg cells upon activation by environmental signals. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Alyce Annie: A New CPR Home Practice Manikin.

ERIC Educational Resources Information Center

Schultz, Alyce; And Others

1981-01-01

Alyce Annie, a lightweight, portable manikin, was designed to economize classroom time and to provide a method for learning cardiopulmonary resuscitation (CPR) independently. A study with high school students determined that the students trained in this method could attain the necessary psychomotor skills and knowledge level required for CPR…

Extended diagnostic criteria for plasmacytoid dendritic cell leukaemia.

PubMed

Garnache-Ottou, Francine; Feuillard, Jean; Ferrand, Christophe; Biichle, Sabeha; Trimoreau, Franck; Seilles, Estelle; Salaun, Véronique; Garand, Richard; Lepelley, Pascale; Maynadié, Marc; Kuhlein, Emilienne; Deconinck, Eric; Daliphard, Sylvie; Chaperot, Laurence; Beseggio, Lucille; Foisseaud, Vincent; Macintyre, Elizabeth; Bene, Marie-Christine; Saas, Philippe; Jacob, Marie-Christine

2009-06-01

The diagnosis of plasmacytoid dendritic cell leukaemia (pDCL) is based on the immunophenotypic profile: CD4(+) CD56(+) lineage(neg) CD45RA(+)/RO(neg) CD11c(neg) CD116(low) CD123(+) CD34(neg) CD36(+) HLA-DR(+). Several studies have reported pDCL cases that do not express this exact profile or expressing some lineage antigens that could thus be misdiagnosed. This study aimed to validate pDCL-specific markers for diagnosis by flow-cytometry or quantitative reverse transcription polymerase chain reaction on bone marrow samples. Expression of markers previously found in normal pDC was analysed in 16 pDCL, four pDCL presenting an atypical phenotype (apDCL) and 113 non-pDC - lymphoid or myeloid - acute leukaemia. CD123 was expressed at significantly higher levels in pDCL and apDCL. BDCA-2 was expressed on 12/16 pDCL and on 2/4 apDCL, but was never detected in the 113 non-pDC acute leukaemia cases. BDCA-4 expression was found on 13/16 pDCL, but also in 12% of non-pDC acute leukaemia. High levels of LILRA4 and TCL1A transcripts distinguished pDCL and apDCL from all other acute leukaemia (except B-cell acute lymphoblastic leukaemia for TCL1A). We thus propose a diagnosis strategy, scoring first the CD4(+) CD56(+/-) MPO(neg) cCD3(neg) cCD79a(neg) CD11c(neg) profile and then the CD123(high), BDCA-2 and BDCA-4 expression. Atypical pDCL can be also identified this way and non-pDC acute leukaemia excluded: this scoring strategy is useful for diagnosing pDCL and apDCL.

A perspective on reagent diversity and non-covalent binding of reactive carbonyl species (RCS) and effector reagents in nonenzymatic glycation (NEG): Mechanistic considerations and implications for future research

NASA Astrophysics Data System (ADS)

Rodnick, Kenneth J.; Holman, R. W.; Ropski, Pamela S.; Huang, Mingdong; Swislocki, Arthur L. M.

2017-06-01

This perspective focuses on illustrating the underappreciated connections between reactive carbonyl species (RCS), initial binding in the nonenzymatic glycation (NEG) process, and nonenzymatic covalent protein modification (here termed NECPM). While glucose is the central species involved in NEG, recent studies indicate that the initially-bound glucose species in the NEG of human hemoglobin (HbA) and human serum albumin (HSA) are non-RCS ring-closed isomers. The ring-opened glucose, an RCS structure that reacts in the NEG process, is most likely generated from previously-bound ring-closed isomers undergoing concerted acid/base reactions while bound to protein. The generation of the glucose RCS can involve concomitantly-bound physiological species (e.g., inorganic phosphate, water, etc.); here termed effector reagents. Extant NEG schemes do not account for these recent findings. In addition, effector reagent reactions with glucose in the serum and erythrocyte cytosol can generate RCS (e.g., glyoxal, glyceraldehyde, etc.). Recent research has shown that these RCS covalently modify proteins in vivo via NECPM mechanisms. A general scheme that reflects both the reagent and mechanistic diversity that can lead to NEG and NECPM is presented here. A perspective that accounts for the relationships between RCS, NEG, and NECPM can facilitate the understanding of site selectivity, may help explain overall glycation rates, and may have implications for the clinical assessment/control of diabetes mellitus. In view of this perspective, concentrations of ribose, fructose, Pi, bicarbonate, counter ions, and the resulting RCS generated within intracellular and extracellular compartments may be of importance and of clinical relevance. Future research is also proposed.

Electron-stimulated desorption from polished and vacuum fired 316LN stainless steel coated with Ti-Zr-Hf-V

DOE Office of Scientific and Technical Information (OSTI.GOV)

Malyshev, Oleg B., E-mail: oleg.malyshev@stfc.ac.uk; Valizadeh, Reza; Hogan, Benjamin T.

2014-11-01

In this study, two identical 316LN stainless steel tubular samples, which had previously been polished and vacuum-fired and then used for the electron-stimulated desorption (ESD) experiments, were coated with Ti-Zr-Hf-V with different morphologies: columnar and dense. ESD measurement results after nonevaporable getter (NEG) activation to 150, 180, 250, and 350 °C indicated that the values for the ESD yields are significantly (2–20 times) lower than the data from our previous study with similar coatings on nonvacuum-fired samples. Based on these results, the lowest pressure and best long-term performance in particle accelerators will be achieved with a vacuum-fired vacuum chamber coated withmore » dense Ti-Zr-Hf-V coating activated at 180 °C. This is likely due to the following facts: after NEG activation, the hydrogen concentration inside the NEG was lower than in the bulk stainless steel substrate; the NEG coating created a barrier for gas diffusion from the sample bulk to vacuum; the dense NEG coating performed better as a barrier than the columnar NEG coating.« less

Pollution Prevention Case Studies: Implications for Army Institutional Processes

DTIC Science & Technology

1994-06-01

waste (FAMC), Optical Fabrication by switching from glass to Laboratory (OFL) plastiC lens production Fort Lewis FORSCOM Develop installation...Role of Compliance Pressures ...................................................................................... 51 6.8 Waste and Recyclables ...Anny Depot AMC Reduce chromium waste (CCAD) through change in Aluminum coating process Fitzsimmons Anny Medical Center HSC Decrease heavy metal

The Annie E. Casey Foundation's New Futures Initiative: Strategic Planning Guide. (Revised).

ERIC Educational Resources Information Center

Center for the Study of Social Policy, Washington, DC.

Information in this guide is based on published research concerning effective strategies in dealing with at-risk youth. It was written for those communities invited to develop proposals under the Annie E. Casey Foundation's New Futures Initiative in an attempt to alleviate problems that compromise American children's futures. Each of three…

Feminizing the Professional: The Government Reports of Flora Annie Steel.

ERIC Educational Resources Information Center

Sutcliffe, Rebecca J.

1998-01-01

Studies Flora Annie Steel, an uneducated woman who nonetheless became an Inspector of Female Schools in Punjab, India, in 1884. Focuses on her reports within the context of British imperialism and late 19th-century report conventions. Concludes that cultural expectations for women in imperialism influenced Steel's response to the genre; and the…

Heterogeneous glycation of cancellous bone and its association with bone quality and fragility.

PubMed

Karim, Lamya; Vashishth, Deepak

2012-01-01

Non-enzymatic glycation (NEG) and enzymatic biochemical processes create crosslinks that modify the extracellular matrix (ECM) and affect the turnover of bone tissue. Because NEG affects turnover and turnover at the local level affects microarchitecture and formation and removal of microdamage, we hypothesized that NEG in cancellous bone is heterogeneous and accounts partly for the contribution of microarchitecture and microdamage on bone fragility. Human trabecular bone cores from 23 donors were subjected to compression tests. Mechanically tested cores as well as an additional 19 cores were stained with lead-uranyl acetate and imaged to determine microarchitecture and measure microdamage. Post-yield mechanical properties were measured and damaged trabeculae were extracted from a subset of specimens and characterized for the morphology of induced microdamage. Tested specimens and extracted trabeculae were quantified for enzymatic and non-enzymatic crosslink content using a colorimetric assay and Ultra-high Performance Liquid Chromatography (UPLC). Results show that an increase in enzymatic crosslinks was beneficial for bone where they were associated with increased toughness and decreased microdamage. Conversely, bone with increased NEG required less strain to reach failure and were less tough. NEG heterogeneously modified trabecular microarchitecture where high amounts of NEG crosslinks were found in trabecular rods and with the mechanically deleterious form of microdamage (linear microcracks). The extent of NEG in tibial cancellous bone was the dominant predictor of bone fragility and was associated with changes in microarchitecture and microdamage.

Heterogeneous Glycation of Cancellous Bone and Its Association with Bone Quality and Fragility

PubMed Central

Karim, Lamya; Vashishth, Deepak

2012-01-01

Non-enzymatic glycation (NEG) and enzymatic biochemical processes create crosslinks that modify the extracellular matrix (ECM) and affect the turnover of bone tissue. Because NEG affects turnover and turnover at the local level affects microarchitecture and formation and removal of microdamage, we hypothesized that NEG in cancellous bone is heterogeneous and accounts partly for the contribution of microarchitecture and microdamage on bone fragility. Human trabecular bone cores from 23 donors were subjected to compression tests. Mechanically tested cores as well as an additional 19 cores were stained with lead-uranyl acetate and imaged to determine microarchitecture and measure microdamage. Post-yield mechanical properties were measured and damaged trabeculae were extracted from a subset of specimens and characterized for the morphology of induced microdamage. Tested specimens and extracted trabeculae were quantified for enzymatic and non-enzymatic crosslink content using a colorimetric assay and Ultra-high Performance Liquid Chromatography (UPLC). Results show that an increase in enzymatic crosslinks was beneficial for bone where they were associated with increased toughness and decreased microdamage. Conversely, bone with increased NEG required less strain to reach failure and were less tough. NEG heterogeneously modified trabecular microarchitecture where high amounts of NEG crosslinks were found in trabecular rods and with the mechanically deleterious form of microdamage (linear microcracks). The extent of NEG in tibial cancellous bone was the dominant predictor of bone fragility and was associated with changes in microarchitecture and microdamage. PMID:22514706

Retaining Low-Income Residents in the Workforce: Lessons from the Annie E. Casey Jobs Initiative.

ERIC Educational Resources Information Center

Giloth, Bob; Gewirtz, Susan

Annie E. Casey Jobs Initiative sites recognize the challenge that long-term retention poses in today's labor market for low-income residents. They have developed key elements of an operational definition of retention, including the following: no limitation to one job, but only very limited gaps between jobs; and jobs in construction or other…

A Chrysanthemum in the Garden: A Christian Kindergarten in the Empire of Japan

ERIC Educational Resources Information Center

Nishida, Yukiyo

2015-01-01

This is a study of the contribution of Christian missionaries to kindergarten education in the Empire of Japan. The study concerns an American Missionary woman, Annie L. Howe (1852-1943) and her kindergarten in Kobe, Japan. Annie L. Howe had a great impact on the history of early childhood education and is still remembered as the "Mother of…

Uterine-derived progenitor cells are immunoprivileged and effectively improve cardiac regeneration when used for cell therapy.

PubMed

Ludke, Ana; Wu, Jun; Nazari, Mansoreh; Hatta, Kota; Shao, Zhengbo; Li, Shu-Hong; Song, Huifang; Ni, Nathan C; Weisel, Richard D; Li, Ren-Ke

2015-07-01

Cell therapy to prevent cardiac dysfunction after myocardial infarction (MI) is less effective in aged patients because aged cells have decreased regenerative capacity. Allogeneic transplanted stem cells (SCs) from young donors are usually rejected. Maintaining transplanted SC immunoprivilege may dramatically improve regenerative outcomes. The uterus has distinct immune characteristics, and we showed that reparative uterine SCs home to the myocardium post-MI. Here, we identify immunoprivileged uterine SCs and assess their effects on cardiac regeneration after allogeneic transplantation. We found more than 20% of cells in the mouse uterus have undetectable MHC I expression by flow cytometry. Uterine MHC I((neg)) and MHC I((pos)) cells were separated by magnetic cell sorting. The MHC I((neg)) population expressed the SC markers CD34, Sca-1 and CD90, but did not express MHC II or c-kit. In vitro, MHC I((neg)) and ((pos)) SCs show colony formation and endothelial differentiation capacity. In mixed leukocyte co-culture, MHC I((neg)) cells showed reduced cell death and leukocyte proliferation compared to MHC I((pos)) cells. MHC I((neg)) and ((pos)) cells had significantly greater angiogenic capacity than mesenchymal stem cells. The benefits of intramyocardial injection of allogeneic MHC I((neg)) cells after MI were comparable to syngeneic bone marrow cell transplantation, with engraftment in cardiac tissue and limited recruitment of CD4 and CD8 cells up to 21 days post-MI. MHC I((neg)) cells preserved cardiac function, decreased infarct size and improved regeneration post-MI. This new source of immunoprivileged cells can induce neovascularization and could be used as allogeneic cell therapy for regenerative medicine. Copyright © 2015 Elsevier Ltd. All rights reserved.

NegGOA: negative GO annotations selection using ontology structure.

PubMed

Fu, Guangyuan; Wang, Jun; Yang, Bo; Yu, Guoxian

2016-10-01

Predicting the biological functions of proteins is one of the key challenges in the post-genomic era. Computational models have demonstrated the utility of applying machine learning methods to predict protein function. Most prediction methods explicitly require a set of negative examples-proteins that are known not carrying out a particular function. However, Gene Ontology (GO) almost always only provides the knowledge that proteins carry out a particular function, and functional annotations of proteins are incomplete. GO structurally organizes more than tens of thousands GO terms and a protein is annotated with several (or dozens) of these terms. For these reasons, the negative examples of a protein can greatly help distinguishing true positive examples of the protein from such a large candidate GO space. In this paper, we present a novel approach (called NegGOA) to select negative examples. Specifically, NegGOA takes advantage of the ontology structure, available annotations and potentiality of additional annotations of a protein to choose negative examples of the protein. We compare NegGOA with other negative examples selection algorithms and find that NegGOA produces much fewer false negatives than them. We incorporate the selected negative examples into an efficient function prediction model to predict the functions of proteins in Yeast, Human, Mouse and Fly. NegGOA also demonstrates improved accuracy than these comparing algorithms across various evaluation metrics. In addition, NegGOA is less suffered from incomplete annotations of proteins than these comparing methods. The Matlab and R codes are available at https://sites.google.com/site/guoxian85/neggoa gxyu@swu.edu.cn Supplementary data are available at Bioinformatics online. © The Author 2016. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

A Strategic Planning Approach: Defining Alternative Counterterrorism Strategies as an Illustration

DTIC Science & Technology

2009-01-01

JUSTICE EDUCATION ENERGY AND ENVIRONMENT HEALTH AND HEALTH CARE INTERNATIONAL AFFAIRS NATIONAL SECURITY POPULATION AND AGING PUBLIC SAFETY SCIENCE AND...Corporation, MR-1738-AF, 2003; Jennifer D. P. Moroney, Nancy E. Blacker, Renee Buhr, James McFadden, Cathryn Quantic Thurston, and Anny Wong...McFadden, Cathryn Quantic Thurston, and Anny Wong, Building Partner Capabilities for Coalition Operations, Santa Monica, Calif.: RAND Corporation, MG-635

Ties That Bind: The Practice of Social Networks. Number Two in a Series of Reports on Social Networks from The Annie E. Casey Foundation

ERIC Educational Resources Information Center

Bailey, Terri J.

2006-01-01

This report documents the Annie E. Casey Foundation's effort to learn from families, communities and organizations around the country about their experiences with social network strategies and approaches. Using the voices and experiences of the families and organizations visited, the report summarizes findings from these visits and helps lay the…

Comparison of Annie Sullivan's Teaching Strategies for Literacy and Communication to the Current Outcome Performance Indicators in Deaf-Blindness: An Exploratory Mixed-Methods Study

ERIC Educational Resources Information Center

Pevsner, Diane

2010-01-01

This study explored teaching strategies for communication and literacy development in deaf-blind students by determining if there was a significant relationship between the instructional strategies practiced by Annie Sullivan in the early 1900s and the contemporary instructional strategies recommended by The National Consortium on Deaf-Blindness…

Benchmarking the Performance of Employment and Training Programs: A Pilot Effort of the Annie E. Casey Foundation's Jobs Initiative.

ERIC Educational Resources Information Center

Welch, Doug

As part of its Jobs Initiative (JI) program in six metropolitan areas Denver, Milwaukee, New Orleans, Philadelphia, St. Louis, and Seattle the Annie E. Casey Foundation sought to develop and test a method for establishing benchmarks for workforce development agencies. Data collected from 10 projects in the JI from April through March, 2000,…

The Annie E. Casey Foundation 2006 Kids Count Pocket Guide. State Profiles of Child Well-Being Series

ERIC Educational Resources Information Center

Annie E. Casey Foundation, 2006

2006-01-01

Kids Count, a project of the Annie E. Casey Foundation, is a national and state by- state effort to track the status of children in the United States. By providing policymakers and citizens with benchmarks of child well-being, Kids Count seeks to enrich local, state, and national discussions concerning ways to secure better futures for all…

Modifying and Supplementing Annie's Project to Increase Impact in New Jersey and Beyond

ERIC Educational Resources Information Center

Brumfield, Robin G.; Carleo, Jenny S.; Kenny, Laura B.; Melendez, Meredith; O'Neill, Barbara; Polanin, Nicholas; Reynolds-Allie, Kenesha

2017-01-01

Annie's Project is a widely known risk management program emphasizing five areas of risk and creating support networks for women in agriculture. Designed as an 18-hr course delivered through a series of face-to-face classes, it can be adapted to meet the learning needs and time constraints of the target audience and instructors. This article…

Maximizing Immune Response to Carbohydrate Antigens on Breast Tumors

DTIC Science & Technology

2004-08-01

selectin binding. In vitro phenotyping of the tumor cells suggests that both KM93-Neg and Pos cells grow at the same rate; however, KM-93Neg cells are...and Figure 2. ABL ( Agaricus bisporus, mushroom, lectin) and ACA (Amaranthus caudatus, lectin) do not react with either the KM93-Pos or KM93-Neg...is different (Figure 6B). The KM93-Pos variant, which is similar to the original 4T1, tends to grow in clusters with high densities. While clusters are

Preparing Young People to Succeed in College and Beyond. The Connection Strategy: Stories and Results from the Annie E. Casey Foundation's Education Investments

ERIC Educational Resources Information Center

Jehl, Jeanne

2007-01-01

For many young people, the "pipeline" to educational and economic success is truly broken. This report describes how some of the Annie E. Casey Foundation's education grantees in Atlanta are working to mend the pipeline for young people in their communities by connecting standards and expectations for learning from early childhood to college entry…

It Is My Desire to Be Free: Annie Davis's Letter to Abraham Lincoln and Winslow Homer's Painting "A Visit from the Old Mistress"

ERIC Educational Resources Information Center

Hussey, Michael; Eder, Elizabeth K.

2010-01-01

"Mr. President, It is my Desire to be free," wrote Annie Davis to Abraham Lincoln, 20 months after he issued the Emancipation Proclamation. The Emancipation Proclamation affected only those parts of the country that were in rebellion against the United States on the date it was issued, January 1, 1863. The slaveholding border states of…

Emergency and Continuous Exposure Limits for Selected Airborne Contaminants. Volume 1

DTIC Science & Technology

1984-04-01

fuels, corn remover, drawing inks, fuel-system deicer, glue, nail-polish remover, paint-brush cleaners, paint and varnish removers, and china ,• ~ ~film...and fire hazards of butanone and acetone. Ind. Bull. (N.Y. State Dept. Labor) 23:173-176. Societa Italiana di Medicina del Lavoro. 1975. Associazione...Italiana di Medicina del Lavoro 1975. Associazione Italiana - -degli Igienisti Industriali. Valori Limite Ponderati degli Inquinanti Chimici e

81. Neg. No. F63, Apr 13, 1930, INTERIORASSEMBLY BUILDING, HOOD ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

81. Neg. No. F-63, Apr 13, 1930, INTERIOR-ASSEMBLY BUILDING, HOOD DEPARTMENT - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

Determinants and prognostic implications of the negative diastolic pulmonary pressure gradient in patients with pulmonary hypertension due to left heart disease.

PubMed

Nagy, Anikó Ilona; Venkateshvaran, Ashwin; Merkely, Béla; Lund, Lars H; Manouras, Aristomenis

2017-01-01

The diastolic pulmonary pressure gradient (DPG) has recently been introduced as a specific marker of combined pre-capillary pulmonary hypertension (Cpc-PH) in left heart disease (LHD). However, its diagnostic and prognostic superiority compared with traditional haemodynamic indices has been challenged lately. Current recommendations explicitly denote that in the normal heart, DPG values are greater than zero, with DPG ≥7 mmHg indicating Cpc-PH. However, clinicians are perplexed by the frequent observation of DPG <0 mmHg (DPG NEG ), as its physiological explanation and clinical impact are unclear to date. We hypothesized that large V-waves in the pulmonary artery wedge pressure (PAWP) curve yielding asymmetric pressure transmission might account for DPG NEG and undertook this study to clarify the physiological and prognostic implications of DPG NEG . Right heart catheterization and echocardiography were performed in 316 patients with LHD due to primary myocardial dysfunction or valvular disease. A total of 256 patients had PH-LHD, of whom 48% demonstrated DPG NEG . The V-wave amplitude inversely correlated with DPG (r = -0.45, P < 0.001) in patients with low pulmonary vascular resistance (PVR), but not in those with elevated PVR (P > 0.05). Patients with large V-waves had negative and lower DPG than those without augmented V-waves (P < 0.001) despite similar PVR (P >0.05). Positive, but normal DPG (0-6 mmHg) carried a worse 2-year prognosis for death and/or heart transplantation than DPG NEG (hazard ratio 2.97; P < 0.05). Our results advocate against DPG NEG constituting a measurement error. We propose that DPG NEG can partially be ascribed to large V-waves and carries a better prognosis than DPG within the normal positive range. © 2016 The Authors. European Journal of Heart Failure © 2016 European Society of Cardiology.

83. Neg. No. F53, Apr 13, 1930, INTERIORASSEMBLY BUILDING, BACK ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

83. Neg. No. F-53, Apr 13, 1930, INTERIOR-ASSEMBLY BUILDING, BACK TRIM LINE - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

85. Neg. No. F51, Apr 13, 1930, INTERIORASSEMBLY BUILDING, BODY ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

85. Neg. No. F-51, Apr 13, 1930, INTERIOR-ASSEMBLY BUILDING, BODY AND CUSHION LINE - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

79. Neg. No. F61A, Apr 13, 1930, INTERIORASSEMBLY BUILDING, BODY ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

79. Neg. No. F-61A, Apr 13, 1930, INTERIOR-ASSEMBLY BUILDING, BODY CONSTRUCTION - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

89. Neg. No. F126, Jul 21, 1931, INTERIORASSEMBLY BUILDING, SHOWING ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

89. Neg. No. F-126, Jul 21, 1931, INTERIOR-ASSEMBLY BUILDING, SHOWING HEADLIGHT TESTING BOOTH - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

92. Neg. No. F142, Mar 29, 1932, INTERIORASSEMBLY BUILDING, STAGE ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

92. Neg. No. F-142, Mar 29, 1932, INTERIOR-ASSEMBLY BUILDING, STAGE AND EXHIBITION ROOM - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

86. Neg. No. F64, Apr 13, 1930, INTERIORASSEMBLY BUILDING, BODY ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

86. Neg. No. F-64, Apr 13, 1930, INTERIOR-ASSEMBLY BUILDING, BODY STORAGE CONVEYOR - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

76. Neg. No. F58, Apr 13, 1930, INTERIORASSEMBLY BUILDING, BURNOFF, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

76. Neg. No. F-58, Apr 13, 1930, INTERIOR-ASSEMBLY BUILDING, BURNOFF, LOAD END OF ENAMEL OVEN - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

77. Neg. No. F65A, Apr 13, 1930, INTERIORASSEMBLY BUILDING, UNLOADING ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

77. Neg. No. F-65A, Apr 13, 1930, INTERIOR-ASSEMBLY BUILDING, UNLOADING THE ENAMEL OVEN - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

90. Neg. No. F59A, Apr 13, 1930, INTERIORASSEMBLY BUILDING, CHASSIS ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

90. Neg. No. F-59A, Apr 13, 1930, INTERIOR-ASSEMBLY BUILDING, CHASSIS LINE, LOOKING SOUTH - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

87. Neg. No. F74A, Jun 14, 1930, INTERIORASSEMBLY BUILDING, BODY ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

87. Neg. No. F-74A, Jun 14, 1930, INTERIOR-ASSEMBLY BUILDING, BODY STORAGE CONVEYORS - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

84. Neg. No. F62, Apr 13, 1930, INTERIORASSEMBLY BUILDING, FRAME ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

84. Neg. No. F-62, Apr 13, 1930, INTERIOR-ASSEMBLY BUILDING, FRAME AND MOTOR STORAGE CONVEYOR - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

74. Neg. No. F116A, May 22, 1931, INTERIORPRESSED STEEL BUILDING, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

74. Neg. No. F-116A, May 22, 1931, INTERIOR-PRESSED STEEL BUILDING, SHOWING FENDER CRATE - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

Adult cardiac stem cells are multipotent and robustly myogenic: c-kit expression is necessary but not sufficient for their identification.

PubMed

Vicinanza, Carla; Aquila, Iolanda; Scalise, Mariangela; Cristiano, Francesca; Marino, Fabiola; Cianflone, Eleonora; Mancuso, Teresa; Marotta, Pina; Sacco, Walter; Lewis, Fiona C; Couch, Liam; Shone, Victoria; Gritti, Giulia; Torella, Annalaura; Smith, Andrew J; Terracciano, Cesare Mn; Britti, Domenico; Veltri, Pierangelo; Indolfi, Ciro; Nadal-Ginard, Bernardo; Ellison-Hughes, Georgina M; Torella, Daniele

2017-12-01

Multipotent adult resident cardiac stem cells (CSCs) were first identified by the expression of c-kit, the stem cell factor receptor. However, in the adult myocardium c-kit alone cannot distinguish CSCs from other c-kit-expressing (c-kit pos ) cells. The adult heart indeed contains a heterogeneous mixture of c-kit pos cells, mainly composed of mast and endothelial/progenitor cells. This heterogeneity of cardiac c-kit pos cells has generated confusion and controversy about the existence and role of CSCs in the adult heart. Here, to unravel CSC identity within the heterogeneous c-kit-expressing cardiac cell population, c-kit pos cardiac cells were separated through CD45-positive or -negative sorting followed by c-kit pos sorting. The blood/endothelial lineage-committed (Lineage pos ) CD45 pos c-kit pos cardiac cells were compared to CD45 neg (Lineage neg /Lin neg ) c-kit pos cardiac cells for stemness and myogenic properties in vitro and in vivo. The majority (~90%) of the resident c-kit pos cardiac cells are blood/endothelial lineage-committed CD45 pos CD31 pos c-kit pos cells. In contrast, the Lin neg CD45 neg c-kit pos cardiac cell cohort, which represents ⩽10% of the total c-kit pos cells, contain all the cardiac cells with the properties of adult multipotent CSCs. These characteristics are absent from the c-kit neg and the blood/endothelial lineage-committed c-kit pos cardiac cells. Single Lin neg c-kit pos cell-derived clones, which represent only 1-2% of total c-kit pos myocardial cells, when stimulated with TGF-β/Wnt molecules, acquire full transcriptome and protein expression, sarcomere organisation, spontaneous contraction and electrophysiological properties of differentiated cardiomyocytes (CMs). Genetically tagged cloned progeny of one Lin neg c-kit pos cell when injected into the infarcted myocardium, results in significant regeneration of new CMs, arterioles and capillaries, derived from the injected cells. The CSC's myogenic regenerative capacity is dependent on commitment to the CM lineage through activation of the SMAD2 pathway. Such regeneration was not apparent when blood/endothelial lineage-committed c-kit pos cardiac cells were injected. Thus, among the cardiac c-kit pos cell cohort only a very small fraction has the phenotype and the differentiation/regenerative potential characteristics of true multipotent CSCs.

82. Neg. No. F66A, Apr 13, 1930, INTERIORASSEMBLY BUILDING, TRIM ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

82. Neg. No. F-66A, Apr 13, 1930, INTERIOR-ASSEMBLY BUILDING, TRIM LINE AND GLASS DEPARTMENTS - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

88. Neg. No. F124, Jul 9, 1931, INTERIORASSEMBLY BUILDING, SHOWING ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

88. Neg. No. F-124, Jul 9, 1931, INTERIOR-ASSEMBLY BUILDING, SHOWING PARTIALLY ASSEMBLED CARS AND FENDER CONVEYORS - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

58. Neg. No. F160a, Jan 11, 1933, INTERIOROFFICE BUILDING/ ASSEMBLY ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

58. Neg. No. F-160a, Jan 11, 1933, INTERIOR-OFFICE BUILDING/ ASSEMBLY BUILDING, FIRST AID ROOM - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

78. Neg. No. F143A, Apr 1, 1932, INTERIORASSEMBLY BUILDING, CONVEYOR ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

78. Neg. No. F-143A, Apr 1, 1932, INTERIOR-ASSEMBLY BUILDING, CONVEYOR LINE WITH CAR CHASSIS - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

56. Neg. No. F161, Mar 18, 1933, INTERIOROFFICE BUILDING, LOBBY ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

56. Neg. No. F-161, Mar 18, 1933, INTERIOR-OFFICE BUILDING, LOBBY AND STAIRWELL, SHOWING DAMAGE AFTER AN EARTHQUAKE - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

DEEPEN: A negation detection system for clinical text incorporating dependency relation into NegEx

PubMed Central

Mehrabi, Saeed; Krishnan, Anand; Sohn, Sunghwan; Roch, Alexandra M; Schmidt, Heidi; Kesterson, Joe; Beesley, Chris; Dexter, Paul; Schmidt, C. Max; Liu, Hongfang; Palakal, Mathew

2018-01-01

In Electronic Health Records (EHRs), much of valuable information regarding patients’ conditions is embedded in free text format. Natural language processing (NLP) techniques have been developed to extract clinical information from free text. One challenge faced in clinical NLP is that the meaning of clinical entities is heavily affected by modifiers such as negation. A negation detection algorithm, NegEx, applies a simplistic approach that has been shown to be powerful in clinical NLP. However, due to the failure to consider the contextual relationship between words within a sentence, NegEx fails to correctly capture the negation status of concepts in complex sentences. Incorrect negation assignment could cause inaccurate diagnosis of patients’ condition or contaminated study cohorts. We developed a negation algorithm called DEEPEN to decrease NegEx’s false positives by taking into account the dependency relationship between negation words and concepts within a sentence using Stanford dependency parser. The system was developed and tested using EHR data from Indiana University (IU) and it was further evaluated on Mayo Clinic dataset to assess its generalizability. The evaluation results demonstrate DEEPEN, which incorporates dependency parsing into NegEx, can reduce the number of incorrect negation assignment for patients with positive findings, and therefore improve the identification of patients with the target clinical findings in EHRs. PMID:25791500

62. Neg. No. F75A, Jun 18, 1930, INTERIORWAREHOUSE, 1ST FLOOR, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

62. Neg. No. F-75A, Jun 18, 1930, INTERIOR-WAREHOUSE, 1ST FLOOR, STORAGE OF AUTOMOBILE COMPONENTS - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

PeoplePersonality: Chris Clarke - a physicist who studies ice cream Teaching Anecdotes: Annie Jump Cannon Obituary: György Marx 1927-2002 Starting Out: What Katie did next: part 3 Opinions: What is really important?

NASA Astrophysics Data System (ADS)

2003-03-01

Featuring relationships, personalities, interactions, environments and reputations involved in physics and education PERSONALITY (156) Chris Clarke - a physicist who studies ice cream TEACHING ANECDOTES (157) Annie Jump Cannon OBITUARY (158) György Marx 1927-2002 Steven Chapman STARTING OUT (159) What Katie did next: part 3 Katie Pennicott OPINIONS (160) What is really important? Kerry Parker

Influence of short-term energy supplementation on estrus, ovarian activity, and blood biochemistry in Ossimi ewes synchronized with fluorogestone acetate in the subtropics.

PubMed

Senosy, W; Mahmoud, G B; Abdel-Raheem, Sh M

2017-01-15

The objective of this study was to evaluate if short-term high-energy diet treatments have any overstimulatory effects on ovarian function and metabolic status in Ossimi ewes synchronized with progesterone sponge. Thirteen ewes were divided into high-energy (HEG; n = 7) and normal-energy or control (NEG; n = 6) groups. Progesterone sponges were placed intravaginally for 14 days during the winter breeding season (December-February). Four days before the removal of the sponges, a high-energy diet (130% of maintenance) was fed to HEG, whereas NEG was offered maintenance diet throughout the experiment. Ovarian performance and progesterone, estradiol, and blood metabolites were assessed daily starting from the day of removal of the sponges. Estrus period was longer in HEG (P < 0.05) when compared with NEG. Ovulation took place considerably longer with larger ovulatory follicles in HEG (P < 0.05). A marked increase in the level of total protein, albumin, glucose, and blood urea during the first 2 days following the removal of progesterone sponge was noticed in HEG when compared with NEG ewes. Eighty-five percentage (85.7%; 6/7) and 66.6% (4/6) had ovulation for the HEG and NEG, respectively. Dietary energy had a nonsignificant effect on the number of the recruited follicles, whereas a significant effect was observed for the diameter of the ovulatory follicle and ovulation rate (HEG, 2.3 ± 0.1 vs. NEG, 1.2 ± 0.3). It is concluded that short-term energy supplementation improves estrus expression and ovarian activity in fluorgestone acetate (FGA)-synchronized Ossimi ewes. Copyright © 2016 Elsevier Inc. All rights reserved.

Protective Cytomegalovirus (CMV)-Specific T-Cell Immunity Is Frequent in Kidney Transplant Patients without Serum Anti-CMV Antibodies.

PubMed

Litjens, Nicolle H R; Huang, Ling; Dedeoglu, Burç; Meijers, Ruud W J; Kwekkeboom, Jaap; Betjes, Michiel G H

2017-01-01

The absence of anti-cytomegalovirus (CMV) immunoglobulin G (IgG) is used to classify pretransplant patients as naïve for CMV infection (CMV neg patients). This study assessed whether pretransplant CMV-specific T-cell immunity exists in CMV neg patients and whether it protects against CMV infection after kidney transplantation. The results show that CMV-specific CD137 + IFNγ + CD4 + and CD137 + IFNγ + CD8 + memory T cells were present in 46 and 39% of CMV neg patients ( n  = 28) although at much lower frequencies compared to CMV pos patients (median 0.01 versus 0.58% for CD4 + and 0.05 versus 0.64% for CD8 + T cells) with a less differentiated CD28-expressing phenotype. In line with these data, CMV-specific proliferative CD4 + and CD8 + T cells were observed in CMV neg patients, which significantly correlated with the frequency of CMV-specific T cells. CMV-specific IgG antibody-secreting cells (ASC) could be detected at low frequency in 36% of CMV neg patients (1 versus 45 ASC/10 5 cells in CMV pos patients). CMV neg patients with pretransplant CMV-specific CD137 + IFNγ + CD4 + T cells had a lower risk to develop CMV viremia after transplantation with a CMV pos donor kidney (relative risk: 0.43, P  = 0.03). In conclusion, a solitary CMV-specific T-cell response without detectable anti-CMV antibodies is frequent and clinically relevant as it is associated with protection to CMV infection following transplantation with a kidney from a CMV pos donor.

From the shadows into the light: How pretrial publicity and deliberation affect mock jurors' decisions, impressions, and memory.

PubMed

Ruva, Christine L; Guenther, Christina C

2015-06-01

This 2-part study explored how exposure to negative pretrial publicity (Neg-PTP) influences the jury process, as well as possible mechanisms responsible for its biasing effects on decisions. Study Part A explored how PTP and jury deliberations affect juror/jury verdicts, memory, and impressions of the defendant and attorneys. One week before viewing a criminal trial mock-jurors (N = 320 university students) were exposed to Neg-PTP or unrelated crime stories (No-PTP). Two days later deliberating jurors came to a group decision, whereas nondeliberating jurors completed an unrelated task before making an individual decision. Neg-PTP jurors were more likely to vote guilty, make memory errors, and rate the defendant lower in credibility. Deliberation reduced Neg-PTP jurors' memory accuracy and No-PTP jurors' guilty verdicts (leniency bias). Jurors' memory and ratings of the defendant and prosecuting attorney significantly mediated the effect of PTP on guilt ratings. Study Part B content analyzed 30 mock-jury deliberations and explored how PTP influenced deliberations and ultimately jury decisions. Neg-PTP juries were more likely than No-PTP juries to discuss ambiguous trial evidence in a proprosecution manner and less likely to discuss judicial instructions and lack of evidence. All Neg-PTP juries mentioned PTP, after instructed otherwise, and rarely corrected jury members who mentioned PTP. Discussion of ambiguous trial evidence in a proprosecution manner and lack of evidence significantly mediated the effect of PTP on jury-level guilt ratings. Together the findings suggest that judicial admonishments and deliberations may not be sufficient to reduce PTP bias, because of memory errors, biased impressions, and predecisional distortion. (c) 2015 APA, all rights reserved).

Influence of a metaphylactic treatment with Baycox(®) Bovis on the reproductive performances of Fresian heifers: a preliminary study.

PubMed

Veronesi, Fabrizia; Nisoli, Lucio; Diaferia, Manuela; Falcini, Roberto; Ficola, Emanuele; Fioretti, Daniela Piergili

2013-06-01

A blind, randomised, controlled, multicentric field trial was conducted to assess the influence of a metaphylactic treatment with an oral solution of toltrazuril on some reproductive parameters of Italian Fresian heifers during the first 18-20 months of life. For this goal 40 calves were selected from two dairy farms and randomly divided into two homogeneous groups: MTol, treated with toltrazuril and NegC, left untreated. The calves were clinically and coprologically examined over the entire study period. The body condition scores, the body weights and the age at the first service were recorded and compared between the two groups, in addition to some other reproductive parameters including number of pregnancies, average service per pregnancy, conception rate, conception rate at first service and post first service conception rate. The analysis of the results showed that the metaphylactic treatment with toltrazuril influenced positively the average age of the first service (MTol 461.4 days versus NegC 485.45 days), the overall conception rate (MTol 95 % versus NegC 85 %), the success at first (MTol 60 % versus NegC 45 %) and second (MTol 75 % versus NegC 45 %) services and, consequently, the mean number of services to be carried out for each animal (MTol 1.4 ± 0.6 versus NegC 1.6 ± 0.79). Furthermore, the results confirmed that toltrazuril treatment, applied in accordance with the epidemiological aspects of each farm, is highly efficacious in persistent reduction of oocyst excretions with particular reference to Eimeria zuernii, Eimeria bovis, considered to be mainly responsible for clinical coccidiosis.

CD26 expression and adenosine deaminase activity in regulatory T cells (Treg) and CD4+ T effector cells in patients with head and neck squamous cell carcinoma

PubMed Central

Mandapathil, Magis; Szczepanski, Miroslaw; Harasymczuk, Malgorzata; Ren, Jin; Cheng, Dongmei; Jackson, Edwin K.; Gorelik, Elieser; Johnson, Jonas; Lang, Stephan; Whiteside, Theresa L

2012-01-01

Adenosine deaminase (ADA) is responsible for the deamination of immunosuppressive adenosine to inosine. In human T lymphocytes, ADA is associated with dipeptidyl peptidase IV (CD26). ADA expression and activity were evaluated in regulatory T cells (Treg) and CD4+ T effector cells (Teff) of patients with head and neck squamous cell cancer (HNSCC). CD4+CD39+ and CD4+CD39neg T cells were isolated by single-cell sorting from the peripheral blood of 15 HNSCC patients and 15 healthy donors (NC). CD26/ADA expression in these cells was studied by multicolor flow cytometry, confocal microscopy, RT-PCR and immunohistochemistry in tumor tissues. ADA activity was evaluated by mass spectrometry, suppression of Teff proliferation in CFSE assays and cytokine production by Luminex. CD4+CD39+ Treg had low and CD4+CD39neg Teff high CD26/ADA expression and ADA activity in NC or HNSCC. The frequency and suppressor activity of CD39+CD26neg Treg were elevated in patients relative to NC (p < 0.01). However, ADA activity in patients’ CD4+CD39neg Teff was decreased (p < 0.05), resulting in extracellular adenosine accumulation. Also, patients’ Teff were more sensitive to inhibitory signals delivered via adenosine receptors. IL-2, IL12 and INFγ upregulated ADA expression and activity in CD4+CD39neg Teff, whereas IL-10, PGE2 and CADO downregulated it. The differentially expressed CD26/ADA can serve as surface markers for functionally-active CD39+CD26neg Treg. PMID:22934258

93. Neg. No. F78K, Aug 11, 1930, EXTERIORASSEMBLY BUILDING, NORTH ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

93. Neg. No. F-78K, Aug 11, 1930, EXTERIOR-ASSEMBLY BUILDING, NORTH SIDE, WITH TAYLOR-TRUCK-A-WAY TRUCKS AND TRAILORS - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

91. Neg. No. F174A, Apr 24, 1936, INTERIORASSEMBLY BUILDING, FINISHED ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

91. Neg. No. F-174A, Apr 24, 1936, INTERIOR-ASSEMBLY BUILDING, FINISHED CARS AT THE END OF THE ASSEMBLY LINE - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

Soft but Strong. Neg-Raising, Soft Triggers, and Exhaustification

ERIC Educational Resources Information Center

Romoli, Jacopo

2012-01-01

In this thesis, I focus on scalar implicatures, presuppositions and their connections. In chapter 2, I propose a scalar implicature-based account of neg-raising inferences, standardly analyzed as a presuppositional phenomenon (Gajewski 2005, 2007). I show that an approach based on scalar implicatures can straightforwardly account for the…

52. Neg. No.none, ca. 1950's, PhotographerUnknown, AERIAL VIEWS OF THE ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

52. Neg. No.-none, ca. 1950's, Photographer-Unknown, AERIAL VIEWS OF THE FORD MOTOR COMPANY ASSEMBLY PLANT, SOMETIME AFTER THE ADDITION OF THE NORTHERN WING - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

51. Neg. No. F183E, Mar 16, 1937, EXTERIORFORD ASSEMBLY PLANT ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

51. Neg. No. F-183E, Mar 16, 1937, EXTERIOR-FORD ASSEMBLY PLANT EAST SIDE, SHOWING OIL WELL #6 IN THE FOREGROUND, NORTHEAST OF THE ASSEMBLY PLANT - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

57. Neg. No. F161D, Mar 18, 1933, INTERIORPRESSED STEEL AND ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

57. Neg. No. F-161D, Mar 18, 1933, INTERIOR-PRESSED STEEL AND WAREHOUSE BUILDINGS, 1ST FLOOR, SHOWING DAMAGE TO FREIGHT ELEVATOR SHAFT FROM EARTHQUAKE - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

49. Neg. No. 7268C, ca. 1930, PhotographerUnknown, OVERALL VIEW OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

49. Neg. No. 7268C, ca. 1930, Photographer-Unknown, OVERALL VIEW OF THE FORD MOTOR COMPANY ASSEMBLY PLANT FROM THE HENRY FORD BRIDGE, PRIOR TO CONSTRUCTION OF THE PRESSED STEEL BUILDING - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

65. Neg. No. F99, Dec 27, 1931, INTERIORPRESSED STEEL BUILDING ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

65. Neg. No. F-99, Dec 27, 1931, INTERIOR-PRESSED STEEL BUILDING FACING SOUTH TOWARD WAREHOUSE AND ASSEMBLY BUILDING 1ST FLOOR, EAST SIDE FACING SOUTH, SHOWING PRESSED STEEL MACHINERY AND RAILROAD CARS - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

94. Neg. No. F130, Sep 24, 1931, EXTERIOROFFICE BUILDING AND ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

94. Neg. No. F-130, Sep 24, 1931, EXTERIOR-OFFICE BUILDING AND ASSEMBLY BUILDING, WEST SIDE, SHOWING TRUCKS AND TRAILORS LOADED WITH NEW TRUCKS DISPLAYING SIGNS 'MORE FORDS FOR HOOVER DAM' - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

50. Neg. No. F176B, Jun 5, 1936, EXTERIORFORD ASSEMBLY PLANT ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

50. Neg. No. F-176B, Jun 5, 1936, EXTERIOR-FORD ASSEMBLY PLANT AT LONG BEACH, OVERALL VIEW OF PLANT FROM THE HENRY FORD BRIDGE, CERRITOS CHANNEL IN THE FOREGROUND - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

CD4+ T Cells Coexpressing CD134 (OX40) Harbor Significantly Increased Levels of Human Herpesvirus 6B DNA Following Umbilical Cord Blood Transplantation.

PubMed

Pritchett, Joshua C; Green, Jaime S; Thomm, Angela M; Knox, Konstance K; Verneris, Michael R; Lund, Troy C

2016-12-15

Human herpesvirus 6B (HHV-6B) commonly reactivates after umbilical cord blood transplantation (UCBT) and is associated with delayed engraftment, fever, rash, and central nervous system dysfunction. Recently, CD134 (OX40) has been implicated as a potential viral entry receptor. We evaluated CD4 + CD134 + / neg-lo and CD8 + CD134 + / neg-lo cells at day 28 after UCBT in 20 subjects with previously documented HHV-6 reactivation and persistent viremia. Analysis of CD4 + CD134 + cells as compared to CD4 + CD134 neg-lo cells showed 0.308 versus 0.129 copies of HHV-6B/cell (P = .0002). CD8 + CD134 +/neg-lo cells contained little to no HHV-6B copies. Following UCBT, CD4 + CD134 + cells harbor significantly increased levels of HHV-6B, suggesting that CD134 (OX40) may facilitate viral entry. © The Author 2016. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail journals.permissions@oup.com.

Characterization of the CEBAF 100 kV DC GaAs Photoelectron Gun Vacuum System

DOE Office of Scientific and Technical Information (OSTI.GOV)

Stutzman, M L; Adderley, P; Brittian, J

A vacuum system with pressure in the low ultra-high vacuum (UHV) range is essential for long photocathode lifetimes in DC high voltage GaAs photoguns. A discrepancy between predicted and measured base pressure in the CEBAF photoguns motivated this study of outgassing rates of three 304 stainless steel chambers with different pretreatments and pump speed measurements of non-evaporable getter (NEG) pumps. Outgassing rates were measured using two independent techniques. Lower outgassing rates were achieved by electropolishing and vacuum firing the chamber. The second part of the paper describes NEG pump speed measurements as a function of pressure through the lower partmore » of the UHV range. Measured NEG pump speed is high at pressures above 5×10 -11 Torr, but may decrease at lower pressures depending on the interpretation of the data. The final section investigates the pump speed of a locally produced NEG coating applied to the vacuum chamber walls. These studies represent the first detailed vacuum measurements of CEBAF photogun vacuum chambers.« less

Evaluation of Ti-Zr-V (NEG) Thin Films for their pumping speed and pumping Capacity

NASA Astrophysics Data System (ADS)

Bansod, Tripti; Sindal, B. K.; Kumar, K. V. A. N. P. S.; Shukla, S. K.

2012-11-01

Deposition of NEG thin films onto the interior walls of the vacuum chambers is an advanced technique to convert a vacuum chamber from a gas source to an effective pump. These films offer considerably large pumping speed for reactive gases like CO, H2 etc. A UHV compatible pumping speed measurement system was developed in-house to measure the pumping speed of NEG coated chambers. To inject the fixed quantity of CO and H2 gas in pumping speed measurement set-up a calibrated leak was also developed. Stainless steel chambers were sputter coated with thin film of Ti-Zr-V getter material using varied parameters for different compositions and thickness. Pumping capacity which is a function of sorbed gas quantities was also studied at various activation temperatures. In order to optimize the activation temperature for maximum pumping speed for CO and H2, pumping speeds were measured at room temperature after activation at different temperatures. The experimental system detail, pumping performance of the NEG film at various activation temperatures and RGA analysis are presented.

Regulation of activation and processing of the cystic fibrosis transmembrane conductance regulator (CFTR) by a complex electrostatic interaction between the regulatory domain and cytoplasmic loop 3.

PubMed

Wang, Guangyu; Duan, Dayue Darrel

2012-11-23

NEG2 regulates CFTR gating but the mechanism is unknown. A putative NEG2-CL3 electrostatic attraction, possibly weakened by Arg-764/Arg-766 of the R domain, prohibited CFTR activation. A charge exchange between NEG2 and CL3 caused misprocessing. Electrostatic regulation of CFTR activation and processing may be asymmetric at the CL3-R interface. The CL3-R interface is optimally designed for multiple regulations of CFTR functions. NEG2, a short C-terminal segment (817-838) of the unique regulatory (R) domain of the cystic fibrosis transmembrane conductance regulator (CFTR) chloride channel, has been reported to regulate CFTR gating in response to cAMP-dependent R domain phosphorylation. The underlying mechanism, however, is unclear. Here, Lys-946 of cytoplasmic loop 3 (CL3) is proposed as counter-ion of Asp-835, Asp-836, or Glu-838 of NEG2 to prevent the channel activation by PKA. Arg-764 or Arg-766 of the Ser-768 phosphorylation site of the R domain is proposed to promote the channel activation possibly by weakening the putative CL3-NEG2 electrostatic attraction. First, not only D835A, D836A, and E838A but also K946A reduced the PKA-dependent CFTR activation. Second, both K946D and D835R/D836R/E838R mutants were activated by ATP and curcumin to a different extent. Third, R764A and R766A mutants enhanced the PKA-dependent activation. However, it is very exciting that D835R/D836R/E838R and K946D/H950D and H950R exhibited normal channel processing and activity whereas D835R/D836R/E838R/K946D/H950D was fractionally misprocessed and silent in response to forskolin. Further, D836R and E838R played a critical role in the asymmetric electrostatic regulation of CFTR processing, and Ser-768 phosphorylation may not be involved. Thus, a complex interfacial interaction among CL3, NEG2, and the Ser-768 phosphorylation site may be responsible for the asymmetric electrostatic regulation of CFTR activation and processing.

Inverse simulation system for manual-controlled rendezvous and docking based on artificial neural network

NASA Astrophysics Data System (ADS)

Zhou, Wanmeng; Wang, Hua; Tang, Guojin; Guo, Shuai

2016-09-01

The time-consuming experimental method for handling qualities assessment cannot meet the increasing fast design requirements for the manned space flight. As a tool for the aircraft handling qualities research, the model-predictive-control structured inverse simulation (MPC-IS) has potential applications in the aerospace field to guide the astronauts' operations and evaluate the handling qualities more effectively. Therefore, this paper establishes MPC-IS for the manual-controlled rendezvous and docking (RVD) and proposes a novel artificial neural network inverse simulation system (ANN-IS) to further decrease the computational cost. The novel system was obtained by replacing the inverse model of MPC-IS with the artificial neural network. The optimal neural network was trained by the genetic Levenberg-Marquardt algorithm, and finally determined by the Levenberg-Marquardt algorithm. In order to validate MPC-IS and ANN-IS, the manual-controlled RVD experiments on the simulator were carried out. The comparisons between simulation results and experimental data demonstrated the validity of two systems and the high computational efficiency of ANN-IS.

Effects of short-term streptozotocin-induced diabetes and vitamin C on platelet non-enzymatic glycation.

PubMed

Batırel, Saime; Yarat, Ayşen; Emekli, Nesrin

2010-01-01

Diabetes mellitus is one of the most prevalent metabolic syndromes worldwide. Glycation, a chemical modification of proteins with reducing sugars, indicates a possible explanation for the association between hyperglycemia and the wide variety of tissue pathologies. Non-enzymatic glycation (NEG) of platelet proteins is one of the key mechanisms in the pathogenesis of diabetic complications and may be significant in diabetic atherothrombosis. The aim of this study was to investigate the effects of streptozotocin (STZ)-induced short-term experimental diabetes on the glycation of platelets and to find out if vitamin C affected this glycation. A total of 40 male Wistar albino rats, 200-250 g, were randomly divided into 4 groups (2 diabetic and 2 control groups). The diabetic groups were made diabetic by intraperitoneal injection of STZ (65 mg/kg, citrate buffer pH 4.5). By daily intraperitoneal injection, 80 mg/kg vitamin C (Roche, Turkey) was administered until the end of the experiment. Blood glucose levels of the diabetic groups were significantly higher than those at day 0 and also higher than those of the non-diabetic control groups. The changes in total protein, NEG and vitamin C levels were not statistically significant. Although the differences among the groups were not statistically significant, vitamin C administration increased NEG levels in the diabetic group. The results of this study demonstrate that 8 days of STZ-induced short-term diabetes did not cause a significant increase in NEG of platelets. However, the effect of vitamin C on platelet NEG needs to be further investigated. Copyright © 2011 S. Karger AG, Basel.

Incorporation of paramagnetic, fluorescent and PET/SPECT contrast agents into liposomes for multimodal imaging

PubMed Central

Mitchell, Nick; Kalber, Tammy L.; Cooper, Margaret S.; Sunassee, Kavitha; Chalker, Samantha L.; Shaw, Karen P.; Ordidge, Katherine L.; Badar, Adam; Janes, Samuel M.; Blower, Philip J.; Lythgoe, Mark F.; Hailes, Helen C.; Tabor, Alethea B.

2013-01-01

A series of metal-chelating lipid conjugates has been designed and synthesized. Each member of the series bears a 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA) macrocycle attached to the lipid head group, using short n-ethylene glycol (n-EG) spacers of varying length. Liposomes incorporating these lipids, chelated to Gd3+, 64Cu2+, or 111In3+, and also incorporating fluorescent lipids, have been prepared, and their application in optical, magnetic resonance (MR) and single-photon emission tomography (SPECT) imaging of cellular uptake and distribution investigated in vitro and in vivo. We have shown that these multimodal liposomes can be used as functional MR contrast agents as well as radionuclide tracers for SPECT, and that they can be optimized for each application. When shielded liposomes were formulated incorporating 50% of a lipid with a short n-EG spacer, to give nanoparticles with a shallow but even coverage of n-EG, they showed good cellular internalization in a range of tumour cells, compared to the limited cellular uptake of conventional shielded liposomes formulated with 7% 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[carboxy(polyethyleneglycol)2000] (DSPE-PEG2000). Moreover, by matching the depth of n-EG coverage to the length of the n-EG spacers of the DOTA lipids, we have shown that similar distributions and blood half lives to DSPE-PEG2000-stabilized liposomes can be achieved. The ability to tune the imaging properties and distribution of these liposomes allows for the future development of a flexible tri-modal imaging agent. PMID:23131536

Identification of an IL-17–producing NK1.1neg iNKT cell population involved in airway neutrophilia

PubMed Central

Michel, Marie-Laure; Keller, Alexandre Castro; Paget, Christophe; Fujio, Masakazu; Trottein, François; Savage, Paul B.; Wong, Chi-Huey; Schneider, Elke; Dy, Michel; Leite-de-Moraes, Maria C.

2007-01-01

Invariant natural killer T (iNKT) cells are an important source of both T helper type 1 (Th1) and Th2 cytokines, through which they can exert beneficial, as well as deleterious, effects in a variety of inflammatory diseases. This functional heterogeneity raises the question of how far phenotypically distinct subpopulations are responsible for such contrasting activities. In this study, we identify a particular set of iNKT cells that lack the NK1.1 marker (NK1.1neg) and secrete high amounts of interleukin (IL)-17 and low levels of interferon (IFN)-γ and IL-4. NK1.1neg iNKT cells produce IL-17 upon synthetic (α-galactosylceramide [α-GalCer] or PBS-57), as well as natural (lipopolysaccharides or glycolipids derived from Sphingomonas wittichii and Borrelia burgdorferi), ligand stimulation. NK1.1neg iNKT cells are more frequent in the lung, which is consistent with a role in the natural immunity to inhaled antigens. Indeed, airway neutrophilia induced by α-GalCer or lipopolysaccharide instillation was significantly reduced in iNKT-cell–deficient Jα18−/− mice, which produced significantly less IL-17 in their bronchoalveolar lavage fluid than wild-type controls. Furthermore, airway neutrophilia was abolished by a single treatment with neutralizing monoclonal antibody against IL-17 before α-GalCer administration. Collectively, our findings reveal that NK1.1neg iNKT lymphocytes represent a new population of IL-17–producing cells that can contribute to neutrophil recruitment through preferential IL-17 secretion. PMID:17470641

Human liver infiltrating γδ T cells are composed of clonally expanded circulating and tissue-resident populations.

PubMed

Hunter, Stuart; Willcox, Carrie R; Davey, Martin S; Kasatskaya, Sofya A; Jeffery, Hannah C; Chudakov, Dmitriy M; Oo, Ye H; Willcox, Benjamin E

2018-05-18

γδ T-cells comprise a substantial proportion of tissue-associated lymphocytes. However, our current understanding of human γδ T-cells is primarily based on peripheral blood subsets, while the immunobiology of tissue-associated subsets remains largely unclear. To address this, we characterised the TCR diversity, immunophenotype and function of human liver infiltrating γδ T-cells, focussing on the predominant tissue-associated Vδ2 neg γδ subset, which is implicated in liver immunopathology. Intrahepatic Vδ2 neg γδ T-cells were highly clonally focussed, with single expanded clonotypes featuring complex, private TCR rearrangements frequently dominating the compartment. Such T-cells were predominantly CD27 lo/neg effector lymphocytes, whereas naïve CD27 hi , TCR diverse populations present in matched blood were generally absent in the liver. Furthermore, while a CD45RA hi Vδ2 neg γδ effector subset present in both liver and peripheral blood contained overlapping TCR clonotypes, the liver Vδ2 neg γδ T-cell pool also included a phenotypically distinct CD45RA lo effector compartment that was enriched for expression of the tissue tropism marker CD69, the hepatic homing chemokine receptors CXCR3 and CXCR6, and liver-restricted TCR clonotypes, suggestive of intrahepatic tissue residency. Liver infiltrating Vδ2 neg γδ cells were capable of polyfunctional cytokine secretion, and unlike peripheral blood subsets, were responsive to both TCR and innate stimuli. These findings suggest the ability of Vδ2 neg γδ T-cells to undergo clonotypic expansion and differentiation is crucial in permitting access to solid tissues such as the liver, and can result in functionally distinct peripheral and liver-resident memory γδ T-cell subsets. They highlight the inherent functional plasticity within the Vδ2 neg γδ T-cell compartment, and may inform design of cellular therapies involving intrahepatic trafficking of γδ T-cells to suppress liver inflammation or combat liver cancer. γδ T cells are frequently enriched in many solid tissues, however the immunobiology of such tissue-associated subsets in humans has remained unclear. We show that intrahepatic γδ T cells are enriched for clonally expanded effector T cells, whereas naïve γδ T cells are largely excluded; moreover, whereas a distinct proportion of circulating T cell clonotypes was present in both the liver tissue and peripheral blood, a functionally and clonotypically distinct population of liver-resident γδ T cells was also evident. Our findings suggest that factors triggering γδ T cell clonal selection and differentiation, such as infection, can drive enrichment of γδ T cells into liver tissue, allowing the development of functionally distinct tissue-restricted memory populations specialised in local hepatic immunosurveillance. Copyright © 2018 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved.

Anti-Candida activity assessment of Pelargonium graveolens oil free and nanoemulsion in biofilm formation in hospital medical supplies.

PubMed

Giongo, Janice Luehring; de Almeida Vaucher, Rodrigo; Fausto, Viviane Pedroso; Quatrin, Priscilla Maciel; Lopes, Leonardo Quintana Soares; Santos, Roberto Christ Vianna; Gündel, André; Gomes, Patrícia; Steppe, Martin

2016-11-01

Infections due to microbial biofilm formation on the surface of catheters and other medical devices are constantly reported as a major cause of morbidity and mortality in patients admitted to hospitals. Furthermore, sessile cells are more resistant to phagocytosis and most antimicrobial, which complicates the treatment of such infections. Researches aimed at new antimicrobial originating mainly from plants have increased in recent years and the development of new strategies for their release is critical in combating the formation of biofilms. Geranium oil (GO) has proven antimicrobial activity. Because of this, the aim of this study was to develop nanoemulsions containing this oil (NEG) and evaluate its activity after the biofilm formation of Candida albicans, Candida tropicalis, Candida glabrata, and Candida krusei in hospital medical supplies. For quantification of the biofilm, crystal violet, total protein, and ATP-bioluminescence assays were used. The results revealed that GO and NEG showed lower MIC for C. albicans and C. tropicalis. The biofilms formed by different species of Candida on the surfaces of polyethylene and polyurethane were quantified. GO and NEG significantly inhibited the formation of biofilms in all species tested on the surfaces of polyethylene. However, NEG antibiofilm has had better activity than GO for C. albicans, C. tropicalis and C. glabrata, according to the surface potential analysis by atomic force microscopy (AFM). The analysis of the biofilm formation on the polyethylene surface by ATP-bioluminescence and CFU showed similar results. In both methods the formation of biofilm in the catheter occurred in greater quantity for C. albicans and C. tropicalis. GO did not significantly inhibit the formation of biofilms only in C. krusei, although NEG significantly increased this activity GO in all species tested when compared to the control training biofilm. The following study shows that the development of NEG may become an effective alternative to reduce the adhesion of microorganisms and prevent infections resulting from the use of some hospital medical materials. Copyright © 2016 Elsevier Ltd. All rights reserved.

The neural correlates of biomechanical constraints in hand laterality judgment task performed from other person's perspective: A near-infrared spectroscopy study.

PubMed

Meng, Shuang; Oi, Misato; Saito, Godai; Saito, Hirofumi

2017-01-01

Previous studies, mainly using a first-person perspective (1PP), have shown that the judgments of the hand laterality judgment (HLJ) task are dependent on biomechanical constraints (BC). Specifically, differing reaction times (RT) for hand pictures rotated medially or laterally around the mid sagittal plane are attributed to the BC effect on motor imagery. In contrast, we investigated whether the HLJ task is also subject to BC when performed from a third-person perspective (3PP) as well as 1PP using near-infrared spectroscopy (NIRS) to measure the brain activity of prefrontal cortex (PFC) in right-handed participants assigned to 1PP or 3PP groups. The 1PP group judged whether a presented hand was their own left or right hand, and the 3PP group whether it was the other's left or right hand. Using their HLJ task error rates, the 1PP and 3PP groups were subdivided into an Error Group (EG) and No Error Group (NEG). For the 1PP group, both EG and NEG showed a significant Hand Laterality × Orientation interaction for RT, indicating the BC effect on motor imagery. For the 3PP group, however, neither EG nor NEG showed the interaction, even though EG showed a significantly longer RT than NEG. These results suggest that the 3PP EG appropriately followed the 3PP task instruction, while the NEG might have taken 1PP. However, the 3PP EG NIRS profile of left PFC showed a significant Hand Laterality × Orientation interaction, while the 1PP EG did not. More noteworthy is that the left PFC activation of EG showed an interaction between the 1PP and 3PP groups when the left hand was presented. Furthermore, in the NEG, the PFC activation was not influenced by the BC in either the 1PP or 3PP condition. These results indicate that BC interferes with the HLJ task performed from the 1PP and 3PP.

Language Policy and Planning: The Case of Italian Sign Language

ERIC Educational Resources Information Center

Geraci, Carlo

2012-01-01

Italian Sign Language (LIS) is the name of the language used by the Italian Deaf community. The acronym LIS derives from Lingua italiana dei segni ("Italian language of signs"), although nowadays Italians refers to LIS as Lingua dei segni italiana, reflecting the more appropriate phrasing "Italian sign language." Historically,…

Computer Simulation Modeling: A Method for Predicting the Utilities of Alternative Computer-Aided Treat Evaluation Algorithms

DTIC Science & Technology

1990-09-01

1988). Current versions of the ADATS have CATE systems insLzlled, but the software is still under development by the radar manufacturer, Contraves ...Italiana, a subcontractor to Martin Marietta (USA). Contraves Italiana will deliver the final version of the software to Martin Marietta in 1991. Until then

Role of antigenemia assay in the early diagnosis and treatment of CMV infection in renal transplant patients.

PubMed

Chiaramonte, S; Pellizzer, G; Rassu, M; Dissegna, D; Bragantiini, L; Zuccarotto, D; La Greca, G

2000-04-01

CMV antigenemia by direct pp65 antigen detection and quantification was monitored on a weekly basis during the first 3 months after kidney transplantation. Preemptive therapy with ganciclovir was started according to the following criteria: any positive antigemia in CMV-NEG subjects, a single determination > or = 30 cell or a two fold increase of positive cells in two consecutive specimens in CMV-POS and continued until pp65 was cleared. Overall, 109 patients were monitored. Among the 24 CMV-NEG patients, 13 (54%) developed a pp65 positive assay without symptoms and were treated. Ten patients remained CMV-infection free and one patient developed late onset (7 months) CMV disease (hepatitis). Among the 85 POS patients 15 (17%) developed a pp65 positive assay and were treated. Two of them developed CMV disease within 7 days of the onset of positive antigenemia and 13 were asymptomatic. The other 70 patients remained CMV-infection free. The interval between transplant and the onset of CMV infection was 39 +/- 13 days in the CMV-NEG group and 64 +/- 20 days in the CMV-POS group (p < 0.001). The peak antigenemia level was 193 +/- 175 cells in the CMV-NEG group and 55+/- 78 cells in the CMV-POS group (p < 0.001). The duration of treatment did not differ in the two groups (22 +/- 7days). A second course of therapy, due to a relapse of asymptomatic infection was performed in 11/13 (85%) treated CMV-NEG patients and in 2/15 (13%) treated CMV-POS patients. Among the total 28 treated patients, we observed only 6 episodes of mild creatinine increase and 9 episodes of mild neutropenia. In the overall population, we observed 8 systemic infections not related to CMV.

Inducible knockdown of pregnancy-associated plasma protein-A gene expression in adult female mice extends life span.

PubMed

Bale, Laurie K; West, Sally A; Conover, Cheryl A

2017-08-01

Pregnancy-associated plasma protein-A (PAPP-A) knockout (KO) mice, generated through homologous recombination in embryonic stem cells, have a significantly increased lifespan compared to wild-type littermates. However, it is unknown whether this longevity advantage would pertain to PAPP-A gene deletion in adult animals. In the present study, we used tamoxifen (Tam)-inducible Cre recombinase-mediated excision of the floxed PAPP-A (fPAPP-A) gene in mice at 5 months of age. fPAPP-A mice, which were either positive (pos) or negative (neg) for Tam-Cre, received Tam treatment with quarterly boosters. Only female mice could be used with this experimental design. fPAPP-A/neg and fPAPP-A/pos mice had similar weights at the start of the experiment and showed equivalent weight gain. We found that fPAPP-A/pos mice had a significant extension of life span (P = 0.005). The median life span was increased by 21% for fPAPP-A/pos compared to fPAPP-A/neg mice. Analysis of mortality in life span quartiles indicated that the proportion of deaths of fPAPP-A/pos mice were lower than fPAPP-A/neg mice at young adult ages (P = 0.002 for 601-800 days) and higher than fPAPP-A/neg mice at older ages (P = 0.004 for >1000 days). Thus, survival curves and age-specific mortality indicate that female mice with knockdown of PAPP-A gene expression as adults have an extended healthy life span. © 2017 The Authors. Aging Cell published by the Anatomical Society and John Wiley & Sons Ltd.

Social anxiety and discomfort with friendly giving.

PubMed

Fernandez, Katya C; Rodebaugh, Thomas L

2011-04-01

Individuals higher in social anxiety report more impaired friendship quality, which past research suggests may stem from constrained warmth. We examined three motivations for constrained warmth in friendships and determined how these motivations related to social anxiety and friendship impairment. To do so, we assessed the psychometric properties of the Favor Scale (FS), which measures an individual's response to friendly giving. Results indicated that the FS has three subscales: negative reactions to favors (NEG), positive reactions to favors (POS), and expectation of tit-for-tat behavior (E-TFT). Structural equation modeling demonstrated that social anxiety related directly to NEG, and indirectly to POS and E-TFT through NEG. POS related directly to friendship quality, indicating that friendships may be impaired in social anxiety disorder due to the cumulative effects of responding negatively to friendly behavior. Copyright © 2010 Elsevier Ltd. All rights reserved.

48. Neg. No. P3120, ca. 1930, Photographer A. C. Gates, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

48. Neg. No. P-3120, ca. 1930, Photographer- A. C. Gates, Los Angeles, AERIAL VIEW OF THE FORD MOTOR COMPANY ASSEMBLY PLANT, PRIOR TO CONSTRUCTION OF THE PRESSED STEEL BUILDING, NOTE THE CLIPPER SHIPS IN THE BACKGROUND, AND THE OIL WELLS IN THE UPPER LEFT CORNER - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

Negative Urgency Mediates the Relationship between Amygdala and Orbitofrontal Cortex Activation to Negative Emotional Stimuli and General Risk-Taking

PubMed Central

Cyders, Melissa A.; Dzemidzic, Mario; Eiler, William J.; Coskunpinar, Ayca; Karyadi, Kenny A.; Kareken, David A.

2015-01-01

The tendency toward impulsive behavior under emotional duress (negative and positive urgency) predicts a wide range of maladaptive risk-taking and behavioral disorders. However, it remains unclear how urgency relates to limbic system activity as induced from emotional provocation. This study used functional magnetic resonance imaging to examine the relationship between brain responses to visual emotional stimuli and urgency traits. Twenty-seven social drinkers (mean age = 25.2, 14 males) viewed negative (Neg), neutral (Neu), and positive (Pos) images during 6 fMRI scans. Brain activation was extracted from a priori limbic regions previously identified in studies of emotional provocation. The right posterior orbitofrontal cortex (OFC) and left amygdala were activated in the [Neg>Neu] contrast, whereas the left posterior OFC was activated in the [Pos>Neu] contrast. Negative urgency was related to the right lateral OFC (r = 0.43, P = 0.03) and the left amygdala (r = 0.39, P = 0.04) [Neg>Neu] activation. Negative urgency also mediated the relationship between [Neg>Neu] activation and general risk-taking (regression weights = 3.42 for right OFC and 2.75 for the left amygdala). Emotional cue-induced activation in right lateral OFC and left amygdala might relate to emotion-based risk-taking through negative urgency. PMID:24904065

Diazepam dose-dependently increases or decreases implicit priming of alcohol associations in problem drinkers.

PubMed

Zack, Martin; Poulos, Constantine X; Woodford, Tracy M

2006-01-01

Words denoting negative affect (NEG) have been found to prime alcohol-related words (ALC) on semantic priming tasks, and this effect is tied to severity of addiction. Previous research suggested that high doses of benzodiazepines may dampen NEG-ALC priming. The present study tested this possibility and the role of motivation for alcohol in this process. A placebo-controlled, double blind, between-within, counterbalanced design was employed. Two groups of male problem drinkers (n = 6/group) received a high (15-mg) or low (5-mg) dose of diazepam versus placebo on two identical test sessions. A lexical decision task assessed priming. Under placebo, significant NEG-->ALC priming emerged in each group. High-dose diazepam selectively reversed this effect, while low-dose selectively enhanced it. Correlations between NEG-->ALC priming and desire for alcohol provided further support that semantic priming of ALC concepts reflects a motivational process. The bi-directional effects found here parallel the effects of high- versus low-dose benzodiazepines on alcohol self-administration in animals. High-dose diazepam reduces prime-induced activation of ALC concepts in problem drinkers. Low-dose diazepam facilitates this process, and cross-priming of motivation for alcohol appears to explain this effect. Neurochemical modulation of the alcohol memory network may contribute to the motivational effects of benzodiazepines in problem drinkers.

School gardens: an experiential learning approach for a nutrition education program to increase fruit and vegetable knowledge, preference, and consumption among second-grade students.

PubMed

Parmer, Sondra M; Salisbury-Glennon, Jill; Shannon, David; Struempler, Barbara

2009-01-01

To examine the effects of a school garden on children's fruit and vegetable knowledge, preference, and consumption. Self-report questionnaires, interview-style taste and rate items, lunchroom observations. An elementary school. Second-grade students (n = 115). Participants were assigned to one of 3 groups: (1) nutrition education and gardening (NE+G) treatment group, (2) nutrition education only (NE) treatment group, or (3) control group (CG). Both treatment groups received classroom instruction, and the NE+G group also received a school gardening experience. Fruit and vegetable knowledge, preference, and consumption. Analyses of variance (alpha = .05). Participants in the NE+G and NE treatment groups exhibited significantly greater improvements in nutrition knowledge and taste ratings than did participants in the CG. Moreover, the NE+G group was more likely to choose and consume vegetables in a lunchroom setting at post-assessment than either the NE or CG groups. School gardens as a component of nutrition education can increase fruit and vegetable knowledge and cause behavior change among children. These findings suggest that school administrators, classroom teachers, and nutrition educators should implement school gardens as a way to positively influence dietary habits at an early age.

[Levels of trace elements in the fruits and vegetables from the so-called Land of fires in Campania Region (Southern Italy)].

PubMed

Esposito, Mauro; Cavallo, Stefania; Rosato, Guido; Chiaravalle, Eugenio; Miedico, Oto; Pellicanò, Roberta; Soprano, Vittorio; Baldi, Loredana

2016-01-01

INTRODUZIONE: una vasta area compresa tra le province di Napoli e Caserta nella regione Campania ha negli ultimi anni attirato l'attenzione dei mass media a causa degli interramenti illeciti di rifiuti di varia origine nei campi o del loro abbandono lungo le strade. A tali pratiche illecite si aggiunge il fenomeno dei roghi incontrollati di questo materiale, motivo per il quale la zona è stata soprannominata "Terra dei fuochi". OBIETTIVI: ricerca di elementi in tracce presenti nei prodotti alimentari di origine vegetale prodotti nella Terra dei fuochi. DISEGNO E SETTING: piano di monitoraggio per il rilevamento dei livelli di alcuni metalli tossici ed essenziali nei vegetali coltivati in Campania nella Terra dei fuochi. La determinazione dei microelementi è stata effettuata dopo mineralizzazione della sostanza organica mediante microonde, utilizzando la spettrometria di massa a plasma induttivamente accoppiato (ICP-MS). RISULTATI: durante l'attività di monitoraggio sono stati raccolti 65 campioni di ortaggi e 49 campioni di frutta in 43 comuni rientranti nella Terra dei fuochi. Il contenuto medio di metalli tossici, cadmio e piombo nei prodotti ortofrutticoli è risultato ben lontano dai limiti massimi fissati dalla normativa europea. I valori delle concentrazioni sono significativamente inferiori rispetto a quanto riscontrato in zone ad alto impatto ambientale a causa della presenza di impianti industriali. CONCLUSIONI: la presenza di elementi tossici o potenzialmente tali nei campioni vegetali prodotti nella Terra dei fuochi è attribuibile a una varietà di fattori ambientali rilevanti (caratteristiche geologiche dell'area, inquinamento del suolo, dell'atmosfera e delle acque di irrigazione, pratiche illecite di smaltimento dei rifiuti industriali); non sembra, tuttavia, sussistere un rischio per la salute dei consumatori. Resta comunque l'allerta per la loro presenza nell'ambiente e la necessità che le attività di monitoraggio continuino.

Neuropsychological Testing in Interventional Cardiology Staff after Long-Term Exposure to Ionizing Radiation.

PubMed

Marazziti, Donatella; Tomaiuolo, Francesco; Dell'Osso, Liliana; Demi, Virginia; Campana, Serena; Piccaluga, Emanuela; Guagliumi, Giulio; Conversano, Ciro; Baroni, Stefano; Andreassi, Maria Grazia; Picano, Eugenio

2015-10-01

This study aimed at comparing neuropsychological test scores in 83 cardiologists and nurses (exposed group, EG) working in the cardiac catheterization laboratory, and 83 control participants (non exposed group, nEG), to explore possible cognitive impairments. The neuropsychological assessment was carried out by means of a battery called "Esame Neuropsicologico Breve." EG participants showed significantly lower scores on the delayed recall, visual short-term memory, and semantic lexical access ability than the nEG ones. No dose response could be detected. EG participants showed lower memory and verbal fluency performances, as compared with nEG. These reduced skills suggest alterations of some left hemisphere structures that are more exposed to IR in interventional cardiology staff. On the basis of these findings, therefore, head protection would be a mandatory good practice to reduce effects of head exposure to ionizing radiation among invasive cardiology personnel (and among other exposed professionals).

Helicobacter pylori Strains from Duodenal Ulcer Patients Exhibit Mixed babA/B Genotypes with Low Levels of BabA Adhesin and Lewis b Binding.

PubMed

Saberi, Samaneh; Schmidt, Alexej; Eybpoosh, Sana; Esmaili, Maryam; Talebkhan, Yeganeh; Mohajerani, Nazanin; Oghalaie, Akbar; Eshagh Hosseini, Mahmoud; Mohagheghi, Mohammad Ali; Bugaytova, Jeanna; Borén, Thomas; Mohammadi, Marjan

2016-10-01

BabA is a Helicobacter pylori cell surface adhesin, which binds to the ABO/Le(b) histo-blood group antigens (Le(b)) and serves as a virulence factor. H. pylori single colonies were isolated from 156 [non-ulcer dyspepsia (NUD) = 97, duodenal ulcer (DU) = 34, gastric cancer (GC) = 25)] patients. babA and babB genes were evaluated by gene/locus-specific PCR. BabA protein expression and Le(b) binding activity were determined by immunoblotting and ELISA, respectively. The combined categorization of H. pylori strains based on high, low or no levels of BabA expression and Le(b) binding, produced 4 groups: (I) BabA-high/Le(b)-high (36 %), (II) BabA-low/Le(b)-low (26 %), (III) BabA-neg/Le(b)-low (30 %) and (IV) BabA-neg/Le(b)-neg (8 %) strains. The majority (63 %) of the BabA-low/Le(b)-low strains exhibited mixed babA/B genotypes as compared to merely 18 % of the BabA-high/Le(b)-high, 15 % of the BabA-neg/Le(b)-neg and 11 % of the BabA-neg/Le(b)-low (P = 0.0001) strains. In contrast to NUD strains, the great majority (70 %) of DU strains were BabA-low/Le(b)-low (11 %, P = 0.0001), which compared to NUD strains, enhanced the risk of DU by 18.8-fold. In parallel, infection with babA/B mixed genotype strains amplified the risk of DU by 3.6-fold (vs. babA-positive: P = 0.01) to 6.9-fold (vs. babA-negative: P = 0.007). Here, we show higher prevalence of mixed babA/B genotypes among BabA-low/Le(b)-low clinical strains. Recombination of babA and babB genes across their loci may yield lower BabA expression and lower Le(b) binding activity. We conclude that H. pylori strains with lower Le(b) binding activity are better adapted for colonization of the gastric metaplastic patches in the duodenum and enhance the risk of duodenal ulcers.

Dynamic Trust Management (DTM)

DTIC Science & Technology

2009-11-04

as the DoD/IC GIG, Navy FORCEnet and Clouds – The ability to define policies for scalable decentralized defense against emergent cyber-threats by... the service you wanted 6. (B) uses (S) Reputation DB 2. How has (B) behaved in the past? (S->B) - Pos: 9 Neg: 1 (S->A) - Pos: 5 Neg: 0 (A->B) - Pos...to create a novel method for trust evaluation The QuanTM Architecture Trust Dependency Graph (TDG), encoding PTM relationships useful for RTM

The formulation of a nasal nanoemulsion zaleplon in situ gel for the treatment of insomnia.

PubMed

Hosny, Khaled Mohamed; Banjar, Zainy Mohammed

2013-08-01

Zaleplon is a drug used for the treatment of insomnia and is available in tablet form; however, it has two major problems. First, the drug undergoes extensive first pass metabolism, resulting in only 30% bioavailability, and second, the drug has a poor aqueous solubility, which delays the onset of action. The objective of this study is to utilise nanotechnology to formulate zaleplon into a nasal in situ nanoemulsion gel (NEG) to provide a solution for the previously mentioned problems. The solubility of zaleplon in various oils, surfactants and co-surfactants was estimated. Pseudo-ternary phase diagrams were developed and various nanoemulsion (NE) formulations were prepared; these formulations were subjected to visual characterisation, thermodynamic stability study and droplet size and conductivity measurements. Carbopol 934 was used as an in situ gelling agent. The gel strength, pH, gelation time, in vitro release and ex vivo nasal permeation were determined. The pharmacokinetic study of the NEG was carried out in rabbits. Stable NEs were successfully developed with a droplet size range of 35 to 73 nm. A NEG composed of 15% Miglyol, 30% Labrasol and 10% PEG 200 successfully provided the maximum in vitro and ex vivo permeation and enhanced the bioavailability in the rabbits by eightfold, when compared with the marketed tablets. The nasal NEG is a promising novel formula for zaleplon that has higher nasal tissue permeability and enhanced systemic bioavailability.

Modified M20 Beam Position Monitor Testing

NASA Astrophysics Data System (ADS)

Koros, Jessica; Musson, John

2017-09-01

Beam position monitors (BPMs) are used to measure lateral beam position. Two pairs of modified wire BPMs are being evaluated for installation into the injector at Jefferson Lab (JLab). The BPMs were coated with a Non-Evaporable Getter (NEG) to aid in pumping at the electron gun, as an ultra-high vacuum is required to protect the gun and to avoid scattering the beam. Beam in the injector has a large diameter, allowing extraction of second moments to give information about beam profile and emittance. The purpose of this project is to determine the effects of NEG coating on the BPMs and to calculate second moments from beam models on the Goubau Line (G-Line). Using the G-Line, scans of the BPMs were taken before and after NEG coating. Each scan produced an electrical field map, which characterizes properties of the BPM, including scale factors and coupling. Second moments were calculated using superposition of previous scan data, and verification of this method was attempted using several beam models. Results show the BPMs responded well to NEG and that measurement of second moments is possible. Once the BPMs are installed, they will enhance gun vacuum and enable monitoring of shape and trajectory of the beam as it exits the electron gun to ensure quality beam for experiments. This work is made possible through support from NSF award 1659177 to Old Dominion University.

Separation of human CD4+CD39+ T cells by magnetic beads reveals two phenotypically and functionally different subsets

PubMed Central

Schuler, Patrick J.; Harasymczuk, Malgorzata; Schilling, Bastian; Lang, Stephan; Whiteside, Theresa L.

2011-01-01

Objective The ectonucleotidase CD39 is an enzyme involved in adenosine production. Its surface expression on human regulatory T cells (Treg) allows for their flow-cytometry-based isolation from peripheral blood. To further develop and improve this method on a scale supporting translational studies, we introduced capture of CD39+ Treg on magnetic immunobeads. Methods Peripheral blood mononuclear cells (PBMC) obtained from healthy donors were used for negative selection of CD4+ T cells on AutoMACS using antibodies (Abs) specific for all lineage+ cells. CD4+CD39+ Treg were captured by biotin-conjugated anti-CD39 Abs and anti-biotin Ab-coated magnetic beads. Isolated CD4+CD39+ T cells were phenotyped by flow cytometry for Treg-associated markers: CD39, CD73, FOXP3, CD25, CTLA-4, CCR4, CD45RO and CD121a or for the absence of CD127 and CD49d. CFSE-based proliferation assays and ATP hydrolysis were used to measure Treg functions. Results The purity, recovery and viability of the separated CD4+CD39+ T cells were satisfactory. The isolated CD4+CD39+ T cell population consisted of FOXP3+CD25+ T cells which hydrolyzed exogenous ATP and suppressed autologous CD4+ T cell proliferation and of FOXP3negCD25neg T cells without suppressor function. The same two subsets were detectable by flow cytometry in normal PBMC, gating on CD4+CD39+, CD4+CD127neg, CD4+CD49dneg or CD4+CD25high Treg. Conclusion CD4+CD39+ Treg capture on immunobeads led to a discovery of two CD39+ subsets. Similar to CD39+ Treg in the peripheral blood, half of these cells are CD25+FOXP3+ active suppressor cells, while the other half are CD25negFOXP3neg and do not mediate suppression. PMID:21513715

Finding Cervical Cancer Symptoms in Swedish Clinical Text using a Machine Learning Approach and NegEx

PubMed Central

Weegar, Rebecka; Kvist, Maria; Sundström, Karin; Brunak, Søren; Dalianis, Hercules

2015-01-01

Detection of early symptoms in cervical cancer is crucial for early treatment and survival. To find symptoms of cervical cancer in clinical text, Named Entity Recognition is needed. In this paper the Clinical Entity Finder, a machine-learning tool trained on annotated clinical text from a Swedish internal medicine emergency unit, is evaluated on cervical cancer records. The Clinical Entity Finder identifies entities of the types body part, finding and disorder and is extended with negation detection using the rule-based tool NegEx, to distinguish between negated and non-negated entities. To measure the performance of the tools on this new domain, two physicians annotated a set of clinical notes from the health records of cervical cancer patients. The inter-annotator agreement for finding, disorder and body part obtained an average F-score of 0.677 and the Clinical Entity Finder extended with NegEx had an average F-score of 0.667. PMID:26958270

Finding Cervical Cancer Symptoms in Swedish Clinical Text using a Machine Learning Approach and NegEx.

PubMed

Weegar, Rebecka; Kvist, Maria; Sundström, Karin; Brunak, Søren; Dalianis, Hercules

2015-01-01

Detection of early symptoms in cervical cancer is crucial for early treatment and survival. To find symptoms of cervical cancer in clinical text, Named Entity Recognition is needed. In this paper the Clinical Entity Finder, a machine-learning tool trained on annotated clinical text from a Swedish internal medicine emergency unit, is evaluated on cervical cancer records. The Clinical Entity Finder identifies entities of the types body part, finding and disorder and is extended with negation detection using the rule-based tool NegEx, to distinguish between negated and non-negated entities. To measure the performance of the tools on this new domain, two physicians annotated a set of clinical notes from the health records of cervical cancer patients. The inter-annotator agreement for finding, disorder and body part obtained an average F-score of 0.677 and the Clinical Entity Finder extended with NegEx had an average F-score of 0.667.

Busulfan is effective second-line therapy for older patients with Philadelphia-negative myeloproliferative neoplasms intolerant of or unresponsive to hydroxyurea.

PubMed

Douglas, Genevieve; Harrison, Claire; Forsyth, Cecily; Bennett, Michael; Stevenson, William; Hounsell, John; Ratnasingam, Sumita; Ritchie, David; Ross, David M; Grigg, Andrew

2017-01-01

Hydroxyurea (Hu) is widely used as first-line cytoreductive therapy for patients with high-risk Philadelphia-negative myeloproliferative neoplasms (Ph-neg MPN), but a small proportion of patients have refractory disease or experience adverse effects. Studies have demonstrated busulfan (Bu) to be an active first-line agent, but data on its role as second-line or later therapy are minimal. To evaluate its efficacy and safety in this context, we undertook a multicenter audit of Ph-neg MPN patients who had received Bu as therapy for Hu intolerance or failure. Of 51 patients identified, 38 (75%) achieved either complete or partial hematological response following at least one Bu cycle. Bu was generally well tolerated, with only 21/135 (15%) cycles complicated by adverse effects, predominantly cytopenia; only 6% of cycles were ceased due to treatment complications. Bu is an effective and well-tolerated agent in patients with Ph-neg MPN in the setting of Hu intolerance or unresponsiveness.

Plasma thymidine kinase-1 activity predicts outcome in patients with hormone receptor positive and HER2 negative metastatic breast cancer treated with endocrine therapy

PubMed Central

Bonechi, Martina; Galardi, Francesca; Biagioni, Chiara; De Luca, Francesca; Bergqvist, Mattias; Neumüller, Magnus; Guarducci, Cristina; Boccalini, Giulia; Gabellini, Stefano; Migliaccio, Ilenia; Di Leo, Angelo; Pestrin, Marta; Malorni, Luca

2018-01-01

The aim of this study was to investigate if thymidine kinase-1 (TK1), a well-known proliferation marker, could represent a valid circulating biomarker to identify hormone receptor positive (HR+)/HER2 negative (HER2neg) metastatic breast cancer (MBC) patients most likely to benefit from endocrine therapy (ET). We used the DiviTum™ assay to analyze TK1 activity in cell lysates of three HR+/HER2neg BC cell lines and in plasma of 31 HR+/HER2neg MBC patients receiving ET. Blood samples were collected at treatment initiation, after one month and at disease progression. CTCs count and ESR1/PIK3CA mutations in circulating tumor DNA were performed and correlated with TK1 activity. TK1 activity was reduced in the two endocrine-sensitive cell lines after 2 days of treatment. In patients, high baseline TK1 activity correlated with CTCs positivity (p-value=0.014). Patients with low baseline levels of TK1 activity had a significantly better PFS compared to those with high baseline TK1 activity (p-value=0.012). Patients with an early drop of TK1 activity after one month of treatment had a significantly better PFS compared to those who experienced an increase (p-value=0.0026). Our study suggests that TK1 could be a potential prognostic, predictive and monitoring marker of early ET response in HR+/HER2neg MBC patients. PMID:29662653

Plasma thymidine kinase-1 activity predicts outcome in patients with hormone receptor positive and HER2 negative metastatic breast cancer treated with endocrine therapy.

PubMed

Bonechi, Martina; Galardi, Francesca; Biagioni, Chiara; De Luca, Francesca; Bergqvist, Mattias; Neumüller, Magnus; Guarducci, Cristina; Boccalini, Giulia; Gabellini, Stefano; Migliaccio, Ilenia; Di Leo, Angelo; Pestrin, Marta; Malorni, Luca

2018-03-27

The aim of this study was to investigate if thymidine kinase-1 (TK1), a well-known proliferation marker, could represent a valid circulating biomarker to identify hormone receptor positive (HR+)/HER2 negative (HER2neg) metastatic breast cancer (MBC) patients most likely to benefit from endocrine therapy (ET). We used the DiviTum™ assay to analyze TK1 activity in cell lysates of three HR+/HER2neg BC cell lines and in plasma of 31 HR+/HER2neg MBC patients receiving ET. Blood samples were collected at treatment initiation, after one month and at disease progression. CTCs count and ESR1 / PIK3CA mutations in circulating tumor DNA were performed and correlated with TK1 activity. TK1 activity was reduced in the two endocrine-sensitive cell lines after 2 days of treatment. In patients, high baseline TK1 activity correlated with CTCs positivity (p-value=0.014). Patients with low baseline levels of TK1 activity had a significantly better PFS compared to those with high baseline TK1 activity (p-value=0.012). Patients with an early drop of TK1 activity after one month of treatment had a significantly better PFS compared to those who experienced an increase (p-value=0.0026). Our study suggests that TK1 could be a potential prognostic, predictive and monitoring marker of early ET response in HR+/HER2neg MBC patients.

Triple negative breast cancer initiating cell subsets differ in functional and molecular characteristics and in γ-secretase inhibitor drug responses

PubMed Central

Azzam, Diana J; Zhao, Dekuang; Sun, Jun; Minn, Andy J; Ranganathan, Prathibha; Drews-Elger, Katherine; Han, Xiaoqing; Picon-Ruiz, Manuel; Gilbert, Candace A; Wander, Seth A; Capobianco, Anthony J; El-Ashry, Dorraya; Slingerland, Joyce M

2013-01-01

Increasing evidence suggests that stem-like cells mediate cancer therapy resistance and metastasis. Breast tumour-initiating stem cells (T-ISC) are known to be enriched in CD44+CD24neg/low cells. Here, we identify two T-ISC subsets within this population in triple negative breast cancer (TNBC) lines and dissociated primary breast cancer cultures: CD44+CD24low+ subpopulation generates CD44+CD24neg progeny with reduced sphere formation and tumourigenicity. CD44+CD24low+ populations contain subsets of ALDH1+ and ESA+ cells, yield more frequent spheres and/or T-ISC in limiting dilution assays, preferentially express metastatic gene signatures and show greater motility, invasion and, in the MDA-MB-231 model, metastatic potential. CD44+CD24low+ but not CD44+CD24neg express activated Notch1 intracellular domain (N1-ICD) and Notch target genes. We show N1-ICD transactivates SOX2 to increase sphere formation, ALDH1+ and CD44+CD24low+cells. Gamma secretase inhibitors (GSI) reduced sphere formation and xenograft growth from CD44+CD24low+ cells, but CD44+CD24neg were resistant. While GSI hold promise for targeting T-ISC, stem cell heterogeneity as observed herein, could limit GSI efficacy. These data suggest a breast T-ISC hierarchy in which distinct pathways drive developmentally related subpopulations with different anti-cancer drug responsiveness. PMID:23982961

Retrieval of radiology reports citing critical findings with disease-specific customization.

PubMed

Lacson, Ronilda; Sugarbaker, Nathanael; Prevedello, Luciano M; Ivan, Ip; Mar, Wendy; Andriole, Katherine P; Khorasani, Ramin

2012-01-01

Communication of critical results from diagnostic procedures between caregivers is a Joint Commission national patient safety goal. Evaluating critical result communication often requires manual analysis of voluminous data, especially when reviewing unstructured textual results of radiologic findings. Information retrieval (IR) tools can facilitate this process by enabling automated retrieval of radiology reports that cite critical imaging findings. However, IR tools that have been developed for one disease or imaging modality often need substantial reconfiguration before they can be utilized for another disease entity. THIS PAPER: 1) describes the process of customizing two Natural Language Processing (NLP) and Information Retrieval/Extraction applications - an open-source toolkit, A Nearly New Information Extraction system (ANNIE); and an application developed in-house, Information for Searching Content with an Ontology-Utilizing Toolkit (iSCOUT) - to illustrate the varying levels of customization required for different disease entities and; 2) evaluates each application's performance in identifying and retrieving radiology reports citing critical imaging findings for three distinct diseases, pulmonary nodule, pneumothorax, and pulmonary embolus. Both applications can be utilized for retrieval. iSCOUT and ANNIE had precision values between 0.90-0.98 and recall values between 0.79 and 0.94. ANNIE had consistently higher precision but required more customization. Understanding the customizations involved in utilizing NLP applications for various diseases will enable users to select the most suitable tool for specific tasks.

Retrieval of Radiology Reports Citing Critical Findings with Disease-Specific Customization

PubMed Central

Lacson, Ronilda; Sugarbaker, Nathanael; Prevedello, Luciano M; Ivan, IP; Mar, Wendy; Andriole, Katherine P; Khorasani, Ramin

2012-01-01

Background: Communication of critical results from diagnostic procedures between caregivers is a Joint Commission national patient safety goal. Evaluating critical result communication often requires manual analysis of voluminous data, especially when reviewing unstructured textual results of radiologic findings. Information retrieval (IR) tools can facilitate this process by enabling automated retrieval of radiology reports that cite critical imaging findings. However, IR tools that have been developed for one disease or imaging modality often need substantial reconfiguration before they can be utilized for another disease entity. Purpose: This paper: 1) describes the process of customizing two Natural Language Processing (NLP) and Information Retrieval/Extraction applications – an open-source toolkit, A Nearly New Information Extraction system (ANNIE); and an application developed in-house, Information for Searching Content with an Ontology-Utilizing Toolkit (iSCOUT) – to illustrate the varying levels of customization required for different disease entities and; 2) evaluates each application’s performance in identifying and retrieving radiology reports citing critical imaging findings for three distinct diseases, pulmonary nodule, pneumothorax, and pulmonary embolus. Results: Both applications can be utilized for retrieval. iSCOUT and ANNIE had precision values between 0.90-0.98 and recall values between 0.79 and 0.94. ANNIE had consistently higher precision but required more customization. Conclusion: Understanding the customizations involved in utilizing NLP applications for various diseases will enable users to select the most suitable tool for specific tasks. PMID:22934127

Effects of parathyroid hormone on cortical porosity, non-enzymatic glycation and bone tissue mechanics in rats with type 2 diabetes mellitus.

PubMed

Campbell, G M; Tiwari, S; Hofbauer, C; Picke, A-K; Rauner, M; Huber, G; Peña, J A; Damm, T; Barkmann, R; Morlock, M M; Hofbauer, L C; Glüer, C-C

2016-01-01

Type 2 diabetes mellitus increases skeletal fragility; however, the contributing mechanisms and the efficacy of bone-forming agents are unclear. We studied diabetes and parathyroid hormone (PTH) treatment effects on cortical porosity (Ct.Po), non-enzymatic glycation (NEG) and bone mechanics in Zucker diabetic fatty (ZDF) rats. Eleven-week old ZDF diabetic (DB) and non-diabetic (ND) rats were given 75μg/kg PTH (1-84) or vehicle 5days per week over 12weeks. The right femora and L4 vertebrae were excised, micro-CT scanned, and tested in 3-point bending and uniaxial compression, respectively. NEG of the samples was determined using fluorescence. Diabetes increased Ct.Po (vertebra (vert): +40.6%, femur (fem): +15.5% vs. ND group, p<0.05) but had no effect on NEG. PTH therapy reduced vertebral NEG in the ND animals only (-73% vs untreated group, p<0.05), and increased femoral NEG in the DB vs. ND groups (+63%, p<0.05). PTH therapy had no effect on Ct.Po. Diabetes negatively affected bone tissue mechanics where reductions in vertebral maximum strain (-22%) and toughness (-42%) were observed in the DB vs. ND group (p<0.05). PTH improved maximum strain in the vertebra of the ND animals (+21%, p<0.05) but did not have an effect in the DB group. PTH increased femoral maximum strain (+21%) and toughness (+28%) in ND and decreased femoral maximum stress (-13%) and toughness (-27%) in the DB animals (treated vs. untreated, p<0.05). Ct.Po correlated negatively with maximum stress (fem: R=-0.35, p<0.05, vert: R=-0.57, p<0.01), maximum strain (fem: R=-0.35, p<0.05, vert: R=-0.43, p<0.05) and toughness (fem: R=-0.34, p<0.05, vert: R=-0.55, p<0.01), and NEG correlated negatively with toughness at the femur (R=-0.34, p<0.05) and maximum strain at the vertebra (R=-0.49, p<0.05). Diabetes increased cortical porosity and reduced bone mechanics, which were not improved with PTH treatment. PTH therapy alone may worsen diabetic bone mechanics through formation of new bone with high AGEs cross-linking. Optimal treatment regimens must address both improvements of bone mass and glycemic control in order to successfully reduce diabetic bone fragility. This article is part of a Special Issue entitled "Bone and diabetes". Copyright © 2015 Elsevier Inc. All rights reserved.

The use of ERTS/LANDSAT imagery in relation to airborne remote sensing for terrain analysis in western Queensland, Australia

NASA Technical Reports Server (NTRS)

Cole, M. M. (Principal Investigator); Owen-Jones, S.

1976-01-01

The author has identified the following significant results. Distinctive spectral signatures were found associated with areas of near surface bedrock with covered ground east of Dugald River and along the Thorntonia River valley west of Lady Annie. Linears identified in the Dugald River area on LANDSAT 2 imagery taken in March and July 1975 over the Cloncurry-Dobbyn area, displayed preferred orientation. A linear group with NE-SW orientation was identified in the Lady Annie area. In this area, the copper mineralization in the Mt. Kelly area occurs along a well marked linear with NNW/SSE direction apparent on images for March, September, and November 1975. Geobotanical anomalies provided surface expression of the copper deposits in Mt. Kelley.

Single Qubit Manipulation in a Microfabricated Surface Electrode Ion Trap (Open Access, Publisher’s Version)

DTIC Science & Technology

2013-09-13

electric fields due to charge build up on the vacuum viewport. For some experiments a non-evaporable getter (NEG) pump is placed 3.3mm away from the...trap, between the trap and the solid aluminum ground shield, to reduce the vacuum pressure close to the ion. The vacuum chamber is constantly pumped by...an ion pump , a titanium sublimation pump and the NEG pump . The pressure of the vacuum system was below what is measurable by the ion gage used (ə.9

Progress Report on Explosives Machining Study

DTIC Science & Technology

1980-08-01

430 ... -7 Pos 0.02 102 74 ... 0.1 0.. ... -1 PBXN -3 Neg 0.02 146 62 0.05 ... ...... -2 Pos 0.01 88 106 ...... ... ,.. -2 HBX-l Neg 0.01 63 63...phys- ical properties. To date, machining experiments have been completed for five explosives TNT, HBX-1, PBXN -3, PBXN -104, and PBXN -105. Equations...This is shown by the range of thermocouple 4t’s, from a low of 10C for TNT, to three times that for PBXN -104. Estimated chip temperatures would be

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Mentoring in Nursing: A Historical Approach.

ERIC Educational Resources Information Center

Fields, Willa L.

1991-01-01

Nursing leaders such as Florence Nightingale, Linda Richards, Mary Adelaide Nutting, and Annie Goodrich were all encouraged by mentors to develop professionally. Most successful professionals have had at least one mentor. (SK)

The Annie Jump Cannon Video Project at the Harvard-Smithsonian Center for Astrophysics.

NASA Astrophysics Data System (ADS)

Lupfer, C.; Welther, B. L.; Griswold, A.

1993-05-01

The heart of this poster paper is the screening of the new 25-minute educational video, ``Annie and the Stars of Many Colors.'' It explores the life and work of Annie Jump Cannon through the eyes of sixth-grade students. A production of the Science Media Group at the CfA, the video was created to interest and inspire girls and minorities, in particular, to continue their study of history and physical science in high school. Recent studies show that science teachers are successfully using videotapes in the classroom to supplement traditional methods of teaching. Other reports show that capable girls and minority students tend to drop science in high school. Our goal, then, was to create a video to stimulate the curiosity and natural interest in science of these younger students. With the help of the Public Affairs Office at the CfA, we arranged to visit local schools to talk to sixth-grade science teachers and their students about the video project. Boys and girls were both eager to participate in it. By lottery, we chose a dozen youngsters of multi-cultural backgrounds to attend a three-day workshop, during which we videotaped them discovering facts about Cannon's childhood and career. Barbara Welther, historian and principal investigator, took the group to the Harvard University Archives to look at some Cannon memorabilia. To learn about spectra, each student assembled a spectroscope from a kit and observed solar lines. CfA astronomers then led the group in various activities to explore the types of stellar spectra that Cannon classified and published in The Henry Draper Catalogue 75 years ago.% and that astronomers still study today. ``Annie and the Stars of Many Colors'' shows young people actively engaged in the process of discovery and offers teachers a novel tool to stimulate discussion of topics in science, history, women's studies, and careers. It is intended for use in schools, libraries, museums, planetariums, as well as for personal interest. For more information and/or a copy of the videotape, contact the authors at the CfA, Cambridge, MA.

A PET reconstruction formulation that enforces non-negativity in projection space for bias reduction in Y-90 imaging

NASA Astrophysics Data System (ADS)

Lim, Hongki; Dewaraja, Yuni K.; Fessler, Jeffrey A.

2018-02-01

Most existing PET image reconstruction methods impose a nonnegativity constraint in the image domain that is natural physically, but can lead to biased reconstructions. This bias is particularly problematic for Y-90 PET because of the low probability positron production and high random coincidence fraction. This paper investigates a new PET reconstruction formulation that enforces nonnegativity of the projections instead of the voxel values. This formulation allows some negative voxel values, thereby potentially reducing bias. Unlike the previously reported NEG-ML approach that modifies the Poisson log-likelihood to allow negative values, the new formulation retains the classical Poisson statistical model. To relax the non-negativity constraint embedded in the standard methods for PET reconstruction, we used an alternating direction method of multipliers (ADMM). Because choice of ADMM parameters can greatly influence convergence rate, we applied an automatic parameter selection method to improve the convergence speed. We investigated the methods using lung to liver slices of XCAT phantom. We simulated low true coincidence count-rates with high random fractions corresponding to the typical values from patient imaging in Y-90 microsphere radioembolization. We compared our new methods with standard reconstruction algorithms and NEG-ML and a regularized version thereof. Both our new method and NEG-ML allow more accurate quantification in all volumes of interest while yielding lower noise than the standard method. The performance of NEG-ML can degrade when its user-defined parameter is tuned poorly, while the proposed algorithm is robust to any count level without requiring parameter tuning.

Diversity of trends of viremia and T-cell markers in experimental acute feline immunodeficiency virus infection.

PubMed

Roche, Sylvain; El Garch, Hanane; Brunet, Sylvie; Poulet, Hervé; Iwaz, Jean; Ecochard, René; Vanhems, Philippe

2013-01-01

The early events of human immunodeficiency virus infection seem critical for progression toward disease and antiretroviral therapy initiation. We wanted to clarify some still unknown prognostic relationships between inoculum size and changes in various immunological and virological markers. Feline immunodeficiency virus infection could be a helpful model. Viremia and T-cell markers (number of CD4, CD8, CD8β(low)CD62L(neg) T-cells, CD4/CD8 ratio, and percentage of CD8β(low)CD62L(neg) cells among CD8 T-cells) were measured over 12 weeks in 102 cats infected with different feline immunodeficiency virus strains and doses. Viremia and T-cell markers trajectory groups were determined and the dose-response relationships between inoculum titres and trajectory groups investigated. Cats given the same inoculum showed different patterns of changes in viremia and T-cell markers. A statistically significant positive dose-response relationship was observed between inoculum titre and i) viremia trajectory-groups (r = 0.80, p<0.01), ii) CD8β(low)CD62L(neg) cell-fraction trajectory-groups (r = 0.56, p<0.01). Significant correlations were also found between viremia and the CD4/CD8 ratio and between seven out of ten T-cell markers. In cats, the infectious dose determines early kinetics of viremia and initial CD8+ T-cell activation. An expansion of the CD8β(low)CD62L(neg) T-cells might be an early predictor of progression toward disease. The same might be expected in humans but needs confirmation.

The relative contributions of non-enzymatic glycation and cortical porosity on the fracture toughness of aging bone

PubMed Central

Tang, S.Y.; Vashishth, D.

2010-01-01

The risk of fracture increases with age due to the decline of bone mass and bone quality. One of the age-related changes in bone quality occurs through the formation and accumulation of advanced glycation end-products (AGEs) due to non-enzymatic glycation (NEG). However as a number of other changes including increased porosity occur with age and affect bone fragility, the relative contribution of AGEs on the fracture resistance of aging bone is unknown. Using a high-resolution nonlinear finite element model that incorporate cohesive elements and micro-computed tomography-based 3d meshes, we investigated the contribution of AGEs and cortical porosity on the fracture toughness of human bone. The results show that NEG caused a 52% reduction in propagation fracture toughness (R-curve slope). The combined effects of porosity and AGEs resulted in an 88% reduction in propagation toughness. These findings are consistent with previous experimental results. The model captured the age-related changes in the R-curve toughening by incorporating bone quantity and bone quality changes, and these simulations demonstrate the ability of the cohesive models to account for the irreversible dynamic crack growth processes affected by the changes in post-yield material behavior. By decoupling the matrix-level effects due to NEG and intracortical porosity, we are able to directly determine the effects of NEG on fracture toughness. The outcome of this study suggests that it may be important to include the age-related changes in the material level properties by using finite element analysis towards the prediction of fracture risk. PMID:21056419

The use of LANDSAT imagery in relation to air survey imagery for terrain analysis in northwest Queensland, Australia, volume 1

NASA Technical Reports Server (NTRS)

Cole, M. M.; Owen-Jones, E. S. (Principal Investigator)

1977-01-01

The author has identified the following significant results. Distinctive spectral signatures discriminated areas underlain by distinctive lithological/stratigraphical units where bedrock either outcrops or is relatively near to surface in the Lady Annie-Mt. Gordon fault zone, the Mary Kathleen, and Dugald River-Naraku areas. Spectral signatures associated with discrete plant communities distinguished different types of superficial deposits over the Cloncurry Plains. Distinctive spectral signatures also revealed the presence and nature of concealed bedrock beneath cover of residuum and superficial deposits where this is relatively thin in the Cloncurry Plains. Major faults were clearly displayed in areas of outcropping and near surface bedrock. Sets of lineaments with preferred orientations were identified in the Lady Annie and Dugald River areas. Known base metal deposits occur along these features.

Advanced Glycation End-products and Bone Fractures.

PubMed

Vashishth, Deepak

2009-08-01

Bone does not turn over uniformly, and becomes susceptible to post-translational modification by non-enzymatic glycation (NEG). NEG of bone causes the formation of advanced glycation end-products (AGEs) and this process is accelerated with aging, diabetes and antiresorptive postmenopausal osteoporosis therapy. Due to the elevated incidence of fracture associated with aging and diabetes, several studies have attempted to measure and evaluate AGEs as biomarkers for fracture risk. Here current methods of estimating AGEs in bone by liquid chromatography and fluorometric assay are summarized and the relationships between AGEs and fracture properties at whole bone, apparent tissue and matrix levels are discussed.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Drakopoulou, Evangelia

The Accelerator Neutrino Neutron Interaction Experiment (ANNIE) is a 26-ton Gd-doped water Cherenkov detector installed in the Booster Neutrino Beam at Fermilab. The experiment has two complementary goals: (1) perform the first measurement of the neutron yield frommore » $$\

Diverging biological roles among human monocyte subsets in the context of tuberculosis infection.

PubMed

Balboa, Luciana; Barrios-Payan, Jorge; González-Domínguez, Erika; Lastrucci, Claire; Lugo-Villarino, Geanncarlo; Mata-Espinoza, Dulce; Schierloh, Pablo; Kviatcovsky, Denise; Neyrolles, Olivier; Maridonneau-Parini, Isabelle; Sánchez-Torres, Carmen; Sasiain, María del Carmen; Hernández-Pando, Rogelio

2015-08-01

Circulating monocytes (Mo) play an essential role in the host immune response to chronic infections. We previously demonstrated that CD16(pos) Mo were expanded in TB (tuberculosis) patients, correlated with disease severity and were refractory to dendritic cell differentiation. In the present study, we investigated whether human Mo subsets (CD16(neg) and CD16(pos)) differed in their ability to influence the early inflammatory response against Mycobacterium tuberculosis. We first evaluated the capacity of the Mo subsets to migrate and engage a microbicidal response in vitro. Accordingly, CD16(neg) Mo were more prone to migrate in response to different mycobacteria-derived gradients, were more resistant to M. tuberculosis intracellular growth and produced higher reactive oxygen species than their CD16(pos) counterpart. To assess further the functional dichotomy among the human Mo subsets, we carried out an in vivo analysis by adapting a hybrid mouse model (SCID/Beige, where SCID is severe combined immunodeficient) to transfer each Mo subset, track their migratory fate during M. tuberculosis infection, and determine their impact on the host immune response. In M. tuberculosis-infected mice, the adoptively transferred CD16(neg) Mo displayed a higher lung migration index, induced a stronger pulmonary infiltration of murine leucocytes expressing pro- and anti-inflammatory cytokines, and significantly decreased the bacterial burden, in comparison with CD16(pos) Mo. Collectively, our results indicate that human Mo subsets display divergent biological roles in the context of M. tuberculosis infection, a scenario in which CD16(neg) Mo may contribute to the anti-mycobacterial immune response, whereas CD16(pos) Mo might promote microbial resilience, shedding light on a key aspect of the physiopathology of TB disease.

The Association of Fat and Lean Tissue With Whole Body and Spine Bone Mineral Density Is Modified by HIV Status and Sex in Children and Youth.

PubMed

Jacobson, Denise L; Lindsey, Jane C; Coull, Brent A; Mulligan, Kathleen; Bhagwat, Priya; Aldrovandi, Grace M

2018-01-01

HIV-infected (HIV-pos) male children/youth showed lower bone mineral density at sexual maturity than HIV-uninfected (HIV-neg) females. It is not known whether complications of HIV disease, including abnormal body fat distribution, contribute to lower bone accrual in male HIV-pos adolescents. In a cross-sectional study, we evaluated the relationship between body composition (fat and lean mass) and bone mass in HIV-pos and HIV-neg children/youth and determined if it is modified by HIV status and sex. We used generalized estimating equations to simultaneously model the effect of fat/lean mass on multiple bone outcomes, including total body bone mineral density and bone mineral content and spine bone mineral density. We evaluated effect modification by HIV and sex. The analysis cohort consisted of 143 HIV-neg and 236 HIV-pos, of whom 55% were black non-Hispanic and 53% were male. Ages ranged from 7 to < 25 years. Half of the children/youth were at Tanner stage 1 and 20% at Tanner 5. Fat mass was more strongly positively correlated with bone mass in HIV-neg than HIV-pos children/youth and these relationships were more evident for total body bone than spine outcomes. Within HIV strata, fat mass and bone were more correlated in female than male children/youth. The relationship between lean mass and bone varied by sex, but not by HIV status. HIV disease diminishes the positive relationship of greater fat mass on bone mass in children/youth. Disruptions in body fat distribution, which are common in HIV disease, may have an impact on bone accretion during pubertal development.

Differentiation "in vitro" of primary and immortalized porcine mesenchymal stem cells into cardiomyocytes for cell transplantation.

PubMed

Moscoso, I; Centeno, A; López, E; Rodriguez-Barbosa, J I; Santamarina, I; Filgueira, P; Sánchez, M J; Domínguez-Perles, R; Peñuelas-Rivas, G; Domenech, N

2005-01-01

Cell transplantation to regenerate injured tissues is a promising new treatment for patients suffering several diseases. Bone marrow contains a population of progenitor cells known as mesenchymal stem cells (MSCs), which have the capability to colonize different tissues, replicate, and differentiate into multilineage cells. Our goal was the isolation, characterization, and immortalization of porcine MSCs (pMSCs) to study their potential differentiation "in vitro" into cardiomyocytes. pMSCs were obtained from the aspirated bone marrow of Large-White pigs. After 4 weeks in culture, adherent cells were phenotypically characterized by flow cytometry and immunochemistry by using monoclonal antibodies. Primary pMSCs were transfected with the plasmid pRNS-1 to obtain continuous growing cloned cell lines. Fresh pMSCs and immortalized cells were treated with 5-azacytidine to differentiate them into cardiomyocytes. Flow cytometry analysis of isolated pMSCs demonstrated the following phenotype, CD90(pos), CD29(pos), CD44(pos), SLA-I(pos), CD106(pos), CD46(pos) and CD45(neg), CD14(neg), CD31(neg), and CD11b(neg), similar to that described for human MSC. We derived several stable immortalized MSC cell lines. One of these, called pBMC-2, was chosen for further characterization. After "in vitro" stimulation of both primary or immortalized cells with 5-azacytidine, we obtained different percentages (30%-50%) of cells with cardiomyocyte characteristics, namely, positive for alpha-Actin and T-Troponin. Thus, primary or immortalized pMSCs derived from bone marrow and cultured were able to differentiate "ex vivo" into cardiac-like muscle cells. These elements may be potentials tools to improve cardiac function in a swine myocardial infarct model.

Genome-wide DNA methylation analysis of pseudohypoparathyroidism patients with GNAS imprinting defects.

PubMed

Rochtus, Anne; Martin-Trujillo, Alejandro; Izzi, Benedetta; Elli, Francesca; Garin, Intza; Linglart, Agnes; Mantovani, Giovanna; Perez de Nanclares, Guiomar; Thiele, Suzanne; Decallonne, Brigitte; Van Geet, Chris; Monk, David; Freson, Kathleen

2016-01-01

Pseudohypoparathyroidism (PHP) is caused by (epi)genetic defects in the imprinted GNAS cluster. Current classification of PHP patients is hampered by clinical and molecular diagnostic overlaps. The European Consortium for the study of PHP designed a genome-wide methylation study to improve molecular diagnosis. The HumanMethylation 450K BeadChip was used to analyze genome-wide methylation in 24 PHP patients with parathyroid hormone resistance and 20 age- and gender-matched controls. Patients were previously diagnosed with GNAS-specific differentially methylated regions (DMRs) and include 6 patients with known STX16 deletion (PHP(Δstx16)) and 18 without deletion (PHP(neg)). The array demonstrated that PHP patients do not show DNA methylation differences at the whole-genome level. Unsupervised clustering of GNAS-specific DMRs divides PHP(Δstx16) versus PHP(neg) patients. Interestingly, in contrast to the notion that all PHP patients share methylation defects in the A/B DMR while only PHP(Δstx16) patients have normal NESP, GNAS-AS1 and XL methylation, we found a novel DMR (named GNAS-AS2) in the GNAS-AS1 region that is significantly different in both PHP(Δstx16) and PHP(neg), as validated by Sequenom EpiTYPER in a larger PHP cohort. The analysis of 58 DMRs revealed that 8/18 PHP(neg) and 1/6 PHP(Δstx16) patients have multi-locus methylation defects. Validation was performed for FANCC and SVOPL DMRs. This is the first genome-wide methylation study for PHP patients that confirmed that GNAS is the most significant DMR, and the presence of STX16 deletion divides PHP patients in two groups. Moreover, a novel GNAS-AS2 DMR affects all PHP patients, and PHP patients seem sensitive to multi-locus methylation defects.

Carboxyhemoglobin levels as a predictor of risk for significant hyperbilirubinemia in African-American DAT(+) infants.

PubMed

Schutzman, D L; Gatien, E; Ajayi, S; Wong, R J

2016-05-01

To compare the degree of hemolysis in a group of direct antiglobulin test (DAT) positive (pos) African-American (AA) infants as measured by carboxyhemoglobin corrected (COHbc) for carbon monoxide in ambient air to a similar group of DAT negative (neg) ABO incompatible infants and a group without blood group incompatibility. To determine if COHbc is a better predictor of significant hyperbilirubinemia than DAT status. A prospective study of 180 AA infants from the Well-Baby Nursery of an inner city community hospital, all of whose mothers were type O pos. Infants (60) were ABO incompatible DAT pos, 60 were ABO incompatible DAT neg and 60 were type O(+). Blood for COHbc was drawn at the time of the infants' initial bilirubin and the infants' precise percentile on the Bhutani nomogram was calculated. Mean COHbc of type O(+) infants was 0.76±0.21 and 0.78±0.24% for ABO incompatible DAT neg infants (P=0.63). Mean CoHbc for the ABO incompatible DAT pos infants was 1.03±0.41% (P<0.0001 compared with both type O and DAT neg infants). Optimal cutoff on the receiver operating characteristic curve for COHbc to determine the risk for being in the Bhutani curve high risk zone was COHbc >0.90% (area under the curve(AUC) 0.8113). This was similar to the AUC of the receiver operating characteristic curve using any titer strength of DAT pos as a cutoff (0.7960). Although not greatly superior to the titer strength of DAT pos, COHbc is useful in determining if the etiology of severe hyperbilirubinemia is a hemolytic process.

Operation of a high-gradient superconducting radio-frequency cavity with a non-evaporable getter pump

DOE PAGES

Ciovati, G.; Geng, R.; Lushtak, Y.; ...

2016-10-28

The use of non-evaporable getter (NEG) pumps in particle accelerators has increased significantly over the past few years because of their large pumping speed, particularly for hydrogen, compared to the size of the pump. A concern about using such pumps in superconducting radio-frequency (SRF) accelerators is the possibility of shedding particulates which could then migrate into the SRF cavities and produce field emission, therefore degrading the cavity performance. One option to mitigate such issue is to use sintered getter materials which intrinsically offer superior mechanical and particle retention properties. In this article we present the results from cryogenic RF testsmore » of a high-gradient SRF cavity after being evacuated several times with an NEG pump equipped with sintered getter disks and placed in close proximity to the cavity. Here, the results showed that the cavity performance was not affected by the pump up to the quench gradient of 34 MV/m. As a result of this study, two such NEG pumps have been installed next to a cryomodule in the CEBAF accelerator to maintain ultra-high vacuum in the SRF cryomodule and two adjacent warm girder sections.« less

Emotional context enhances auditory novelty processing in superior temporal gyrus.

PubMed

Domínguez-Borràs, Judith; Trautmann, Sina-Alexa; Erhard, Peter; Fehr, Thorsten; Herrmann, Manfred; Escera, Carles

2009-07-01

Visualizing emotionally loaded pictures intensifies peripheral reflexes toward sudden auditory stimuli, suggesting that the emotional context may potentiate responses elicited by novel events in the acoustic environment. However, psychophysiological results have reported that attentional resources available to sounds become depleted, as attention allocation to emotional pictures increases. These findings have raised the challenging question of whether an emotional context actually enhances or attenuates auditory novelty processing at a central level in the brain. To solve this issue, we used functional magnetic resonance imaging to first identify brain activations induced by novel sounds (NOV) when participants made a color decision on visual stimuli containing both negative (NEG) and neutral (NEU) facial expressions. We then measured modulation of these auditory responses by the emotional load of the task. Contrary to what was assumed, activation induced by NOV in superior temporal gyrus (STG) was enhanced when subjects responded to faces with a NEG emotional expression compared with NEU ones. Accordingly, NOV yielded stronger behavioral disruption on subjects' performance in the NEG context. These results demonstrate that the emotional context modulates the excitability of auditory and possibly multimodal novelty cerebral regions, enhancing acoustic novelty processing in a potentially harming environment.

Self-rostering can improve work-life balance and staff retention in the NHS.

PubMed

Barrett, Renee; Holme, Annie

2018-03-08

Renee Barrett, Staff Nurse, ITU, Great Ormond Street Hospital NHS Trust, renee.barrett@gosh.nhs.uk , and Annie Holme, Lecturer, King's College London, look at how e-rostering can benefit health organisations and staff.

Il ruolo svolto dall’attività lavorativa sull’ipertensione arteriosa

PubMed Central

Barbini, N.; Gorini, G.; Ferrucci, L.; Biggeri, A.

2009-01-01

RIASSUNTO Introduzione I parametri di pressione arteriosa, sistolica (PAS) o diastolica (PAD) hanno costituito I’oggetto di numerosi studi, alcuni dei quali stabiliscono un’associazione tra I’aumnto di tali parametri e la mortalità, in particolare alle età medie. I fattori di rischio di ipertensione più frequentemente studiati sono il consumo di alcol, il fumo e I’origine etnica in riferimento sia alle differenze genetiche che nella condotta alimentare, mentre i fattori di origine professionale sono stati presi in considerazione più raramente e in maniera poco approfondita, inoltre sono state riferite notevoli differenze nelle pressioni arteriose medie di individui che svolgono professioni diverse. Sembra infatti che i “colletti blu” siano più a rischio dei “colletti bianchi”. Obiettivi Il nostro scopo è valutare se determinati rischi o vincoli connessi con l’attività professionale o determinati stili di vita rappresentano un fattore di rischio per l’ipertensione arteriosa in considerazioni dell’aumentare dell’età. Elementi specifici che rendano conto di questa relazione non sono stati ancora completamente chiariti e soprattutto i dati sulla popolazione italiana sono molto limitati. Metodi La metodologia di ricerca utilizzata è stata quella dell’indagine francese ESTEV (Derriennic, Touranchet, Volkoff) - VISAT (Marquie, Janson), studio epidemiologico longitudinale su oltre 23000 lavoratori attivata in Francia nel 1990 al fine di indagare gli effetti a lungo termine delle condizioni di lavoro, riferite all’intera vita professionale, sia sull’invecchiamento sia sulla salute dei lavoratori. Analoga indagine è stata avviata in Italia, nel 2000, in 6 Regioni, prendendo in esame i lavoratori dipendenti di diversi settori produttivi appartenenti a 5 coorti di età: da 32 a 52 anni con intervallo di 5 anni. Le rilevazioni sono state effettuate dai medici del lavoro di azienda attraverso 3 questionari: 1. sulle condizioni professionali (esposizione a determinati rischi o vincoli); 2. sugli stili di vita e la salute percepita secondo il Nottingham Health Profile; 3. sulle condizioni oggettive di salute. Per il presente lavoro saranno considerati solo alcuni parametri utili all’approfondimento epidemiologico nel settore cardiocircolatorio. Essi riguarderanno: 1) le condizioni di lavoro, 2) la valutazione soggettiva del lavoro, 3) costrizioni specifiche del lavoro vissute come più gravose con l’avanzare dell’età. Relativamente all’ipertensione, sono stati considerati ipertesi i soggetti che presentavano una PAS ≥160 mm/Hg e/o una PAD ≥90 mm/Hg nonché coloro in cura antipertensiva. Risultati I risultati si riferiscono a 1104 lavoratori, di cui 76% uomini e 24% donne. La prevalenza di ipertensione si è dimostrata più elevata nei maschi (33% Vs 22%). Le stime del rischio hanno evidenziato una associazione statisticamente significativa con alcuni fattori lavorativi come mantenere posture difficili (O.R. 1.71), lavorare in presenza di calore (O.R. 1.43), a contatto con agenti chimici (O.R, 1.31). stare a lungo seduti (O.R. 1.48), fare più cose contemporaneamente (O.R. 1.41), essere interrotti nel proprio lavoro (O.R. 1,35). non poter distogilere gli occhi dal lavoro (O.R.1.61). Conclusioni Noi abbiamo osservato che alcune caratteristiche fisiche del lavoro, ma soprattutto quelle di tipo organizzativo sono fortemente associate all’ipertensione arteriosa. Un elemento importante è sembrata la valutazione soggettiva del valore che i lavoratori danno del proprio lavoro: tale elemento è fortemente connesso con I’organizzazione del lavoro, pertanto è un possibile target di prevenzione (fattore di rischio modificabile). PMID:17886759

Transungual Delivery of Ketoconazole Nanoemulgel for the Effective Management of Onychomycosis.

PubMed

Mahtab, Asiya; Anwar, Mohammed; Mallick, Neha; Naz, Zrien; Jain, Gaurav K; Ahmad, Farhan J

2016-12-01

Ketoconazole (KCZ) nanoemulgel containing permeation enhancer was formulated as a vehicle for transungual drug delivery, and its efficacy to inhibit the growth of onychomycotic dermatophytes was investigated in vitro. Different components of oil-in-water nanoemulsions were moderately agitated by classical titration method and passed through a high-pressure homogenizer to formulate various nanoemulsions, which were further identified by constructing pseudo-ternary phase diagrams. Stress-stability testing was carried out for the nanoemulsions, and those that passed these tests were characterized for mean droplet size, zeta potential, morphology, pH, refractive index, viscosity and transmittance. Mean droplet size and zeta potential of the optimized nanoemulsion (NE3) were found to be 77.52 ± 0.92 nm (polydispersity index (PDI) = 0.128 ± 0.035) and -5.44 ± 0.67 mV, respectively. Optimized nanoemulsion was converted into nanoemulgel (NEG 1 ) with 1% (w/w) of gelling agent (Carbopol® Ultrez 21) and 1%-2% (v/v) thioglycolic acid as permeation enhancer, and evaluated for pH, viscosity, spreadability, extrudability, tensile strength and bio-adhesion measurement. In vitro cumulative drug released at the end of 24 h from NE3, NEG 1 and drug suspension were found to be 98.87 ± 1.29, 84.42 ± 2.78% and 54.86 ± 2.19%, respectively. Ex vivo transungual permeation values for KCZ through goat hooves from NE3, NEG 1 and drug suspension were found to be 62.49 ± 2.98, 77.54 ± 2.88% and 38.54 ± 2.54%, respectively, in 24 h. The antifungal effect of NEG 1 on Trichophyton rubrum and Candida albicans showed a significant (p < 0.05) zone of inhibition as compared to drug solution. Skin irritation and histopathology studies on rat skin showed the safe topical use and enhanced permeation of formulated nanoemulgel.

Health evaluation of pronghorns (Antilocapra americana) on Hart Mountain National Antelope Refuge in southeastern Oregon, 1996-1997.

USGS Publications Warehouse

Dunbar, Michael R.; Velarde, Roser

1998-01-01

One hundred four neonatal (fawns) and 40 adult female (does) pronghorn antelope (pronghorns) (Antilocapra americana) were captured on the Hart Mountain National Antelope Refuge (HMNAR) in Lake County, southeastern Oregon, between 13 May 1996 and 26 May 1997. Blood and fecal samples were taken for an investigation of low fawn survival that may be due to disease and/or poor nutrition. No abnormalities were found in hematological parameters of adult does (n = 40) or fawns (n = 44 to 67). In general, there were lower serum total proteins (TP) and Blood Urea Nitrogen (BUN) concentrations in this population than in other populations from Alberta, Canada; Idaho; and Baker City, Oregon. Mean BUN values in does were significantly lower (p < 0.001) in December than March. The duration of this apparently low protein content of the December diet may affect the weight of the newborn and consequent survivability if it should continue into late gestation (March-May). Serum copper (Cu) levels in does (range 0.39 to 0.74 ppm) were considered marginal when compared to domestic animals and some wild ungulates. Fawns had low but apparently normal Cu levels at birth and reached the does' marginal values in about three days. Whole blood and serum Selenium (Se) levels (<100 ng/ml) were considered to be marginal to low in most segments of the pronghorn population in this study. However, serum levels of vitamin E (range 1.98 - 3.27 pg/ml), as determined from the does captured in March, are apparently sufficient to offset any signs of deficiency due to low Se levels. No clinical signs of Cu or Se deficiency were observed. Does captured in December 1996 were tested for neutralizing antibodies to Brucella spp. (n = 20, neg.), Leptospira spp. (n = 20, neg.), bluetongue virus (n = 20, 35% pos.), epizootic hemorrhagic disease virus (n = 20, 30% pos.), respiratory syncytial virus (n = 18, neg.), parainfluenza virus type 3 (n = 18, 67% pos.), infectious bovine rhinotracheitis (n = 18, neg.), and bovine viral diarrhea (n = 18, neg.). Seventeen fawns (9F,8M), nine in 1996 and eight in 1997, survived until at least mid-July each year. Fifty-five of 87 dead fawns were necropsied. Predation by coyotes (Canis latrans) accounted for the majority of fawn mortality (62%), as determined by necropsy, during the two combined summer periods. Other causes of mortality for the combined years included predation by eagle (4%), dystocia (2%), septicemic pasteurellosis (5%), starvation/weak fawn syndrome

The role of immune cell subpopulations in the growth and rejection of TC-1/A9 tumors in novel mouse strains differing in the H2-D haplotype and NKC domain.

PubMed

Indrová, Marie; Rossowska, Joanna; Pajtasz-Piasecka, Elzbieta; Mikyšková, Romana; Richter, Jan; Rosina, Jozef; Sedlacek, Radislav; Fišerová, Anna

2018-03-01

The present study aimed to elucidate the role of cluster of differentiation (CD)8+, CD4+, natural killer (NK), and myeloid (CD11b+) cells in the course of the growth and rejection of experimental major histocompatibility complex (MHC) class I-deficient, HPV16 E6/E7-associated TC-1/A9 tumors in mice. Stable mouse lines (F 30 ) generated by inbreeding of Balb/c and C57BL/6 strains, which were characterized by H-2Db+d-NK1.1neg (B6-neg) and H-2Db-d+NK1.1high (Balb-high) phenotypes, were used for the present study. The novel strains spontaneously regressed tumors in 70-90% of cases. Ex vivo histological analysis of the tumor microenvironment in cryosections showed an indirect correlation between the growth of the transplanted tumor (progressor vs. regressor mice) and the proportion of immunocompetent cell infiltration in the tumors. The regressor mice exhibited a higher infiltration of tumors with CD4+ and CD8+ cells, and in Balb-high with NK cells as well, compared with the progressors. All tumor transplants also indicated a huge infiltration of CD11b+ cells, but this infiltration was not dependent on the stage of the TC-1/A9 tumor development. Depletion of individual cell subpopulations in vivo exhibited different effects on the tumor development in the two strains. Elimination of CD8-positive cells enhanced growth of TC-1/A9 tumor transplants in both hybrid stains, whereas CD4+ cell depletion affected rejection of TC-1/A9 tumors in the B6-neg mice only. Depletion of NK cells with anti-asialo GM1 antibody in the Balb-high strain led to enhancement of tumor growth, which was more pronounced after depletion of the NK1.1+ subpopulation. On the other hand, depletion of NK cells with anti-asialo GM1 in B6-neg mice did not affect the regression of TC-1/A9 tumor transplants, but increased the CD11b+ cell infiltration. In summary, these results indicate that co-operation of particular subsets of immunocompetent cells is essential for the rejection of TC-1/A9 tumor transplants. In B6-neg mice, the co-operative action of CD8+ and CD4+ cells is required, whereas in Balb-high mice, the synergy of CD8+ and NK1.1+ cells is of major importance.

EpCAM-Independent Enrichment of Circulating Tumor Cells in Metastatic Breast Cancer.

PubMed

Schneck, Helen; Gierke, Berthold; Uppenkamp, Frauke; Behrens, Bianca; Niederacher, Dieter; Stoecklein, Nikolas H; Templin, Markus F; Pawlak, Michael; Fehm, Tanja; Neubauer, Hans

2015-01-01

Circulating tumor cells (CTCs) are the potential precursors of metastatic disease. Most assays established for the enumeration of CTCs so far-including the gold standard CellSearch-rely on the expression of the cell surface marker epithelial cell adhesion molecule (EpCAM). But, these approaches may not detect CTCs that express no/low levels of EpCAM, e.g. by undergoing epithelial-to-mesenchymal transition (EMT). Here we present an enrichment strategy combining different antibodies specific for surface proteins and extracellular matrix (ECM) components to capture an EpCAMlow/neg cell line and EpCAMneg CTCs from blood samples of breast cancer patients depleted for EpCAM-positive cells. The expression of respective proteins (Trop2, CD49f, c-Met, CK8, CD44, ADAM8, CD146, TEM8, CD47) was verified by immunofluorescence on EpCAMpos (e.g. MCF7, SKBR3) and EpCAMlow/neg (MDA-MB-231) breast cancer cell lines. To test antibodies and ECM proteins (e.g. hyaluronic acid (HA), collagen I, laminin) for capturing EpCAMneg cells, the capture molecules were first spotted in a single- and multi-array format onto aldehyde-coated glass slides. Tumor cell adhesion of EpCAMpos/neg cell lines was then determined and visualized by Coomassie/MitoTracker staining. In consequence, marginal binding of EpCAMlow/neg MDA-MB-231 cells to EpCAM-antibodies could be observed. However, efficient adhesion/capturing of EpCAMlow/neg cells could be achieved via HA and immobilized antibodies against CD49f and Trop2. Optimal capture conditions were then applied to immunomagnetic beads to detect EpCAMneg CTCs from clinical samples. Captured CTCs were verified/quantified by immunofluorescence staining for anti-pan-Cytokeratin (CK)-FITC/anti-CD45 AF647/DAPI. In total, in 20 out of 29 EpCAM-depleted fractions (69%) from 25 metastatic breast cancer patients additional EpCAMneg CTCs could be identified [range of 1-24 CTCs per sample] applying Trop2, CD49f, c-Met, CK8 and/or HA magnetic enrichment. EpCAMneg dual-positive (CKpos/CD45pos) cells could be traced in 28 out of 29 samples [range 1-480]. By single-cell array-based comparative genomic hybridization we were able to demonstrate the malignant nature of one EpCAMneg subpopulation. In conclusion, we established a novel enhanced CTC enrichment strategy to capture EpCAMneg CTCs from clinical blood samples by targeting various cell surface antigens with antibody mixtures and ECM components.

Bonding Social Capital in Low-Income Neighborhoods

ERIC Educational Resources Information Center

Brisson, Daniel S.; Usher, Charles L.

2005-01-01

Social capital has recently become a guiding theoretical framework for family interventions in low-income neighborhoods. In the context of the Annie E. Casey Foundation's Making Connections initiative, this research uses hierarchical linear modeling to examine how neighborhood characteristics and resident participation affect bonding social…

From golden hills to sycamore trees: pastoral homelands and ethnic identity in Irish immigrant fiction, 1860-75.

PubMed

Corporaal, Marguérite

2010-01-01

The prose fiction that remembers the trials of starvation and eviction of the Great Famine (1845-50) often juxtaposes representations of blasted, infertile land with images of a green, idyllic Erin. Through a discussion of Mary Anne Sadlier's Bessy Conway (1861), Elizabeth Hely Walshe's Golden Hills: A Tale of the Irish Famine (1865) and John McElgun's Annie Reilly (1873), this article reveals that immigrant writers of the Famine generation often negotiate depictions of Famine-stricken wasteland with evocations of a pastoral homeland. In the case of the two Catholic novels, Bessy Conway and Annie Reilly, the pastoral becomes a point of ethnic identification through which the immigrants can recollect and reconstruct a sense of Irishness in exile. By contrast, Golden Hills, which focuses on the Anglo-Irish ascendancy, does not lament the mass exodus of afflicted Irish: the novel rather envisions emigration as a way to regenerate Ireland as locus amoenus.

Telangiectatic osteosarcoma of the rib: a rare entity and a potential diagnostic pitfall.

PubMed

Saguem, I; Ayadi, L; Kallel, R; Charfi, S; Bahri, I; Gouiaa, N; Sellami-Boudawara, T

2016-12-01

Osteosarcoma (OS) is a common primary malignant tumor of bones that produces osteoid matrix. Telangiectatic osteosarcoma (TOS) is a rare variant of OS. It affects the long bones especially the lower end of femur and the upper ends of tibia and humerus, a distribution similar to the conventional osteosarcoma. The rib involvement is very infrequent. We present a case of TOS of the rib that posed a diagnostic difficulty owing to its unusual location and to its resemblance to giant cell tumor and aneurysmal bone cyst. Correspondence. © Copyright Società Italiana di Anatomia Patologica e Citopatologia Diagnostica, Divisione Italiana della International Academy of Pathology.

Management of patients with cardiac implantable electronic devices (CIED) undergoing radiotherapy: A consensus document from Associazione Italiana Aritmologia e Cardiostimolazione (AIAC), Associazione Italiana Radioterapia Oncologica (AIRO), Associazione Italiana Fisica Medica (AIFM).

PubMed

Zecchin, Massimo; Severgnini, Mara; Fiorentino, Alba; Malavasi, Vincenzo Livio; Menegotti, Loris; Alongi, Filippo; Catanzariti, Domenico; Jereczek-Fossa, Barbara Alicja; Stasi, Michele; Russi, Elvio; Boriani, Giuseppe

2018-03-15

The management of patients with a cardiac implanted electronic device (CIED) receiving radiotherapy (RT) is challenging and requires a structured multidisciplinary approach. A consensus document is presented as a result of a multidisciplinary working group involving cardiac electrophysiologists, radiation oncologists and physicists in order to stratify the risk of patients with CIED requiring RT and approaching RT sessions appropriately. When high radiation doses and beam energy higher than 6MV are used, CIED malfunctions can occur during treatment. In our document, we reviewed the different types of RT and CIED behavior in the presence of ionizing radiations and electromagnetic interferences, from the cardiologist's, radiation oncologist's and medical physicist's point of view. We also reviewed in vitro and in vivo literature data and other national published guidelines on this issue so far. On the basis of literature data and consensus of experts, a detailed approach based on risk stratification and appropriate management of RT patients with CIEDs is suggested, with important implications for clinical practice. Copyright © 2017 Elsevier Ireland Ltd. All rights reserved.

Advanced Motor-Controller Development.

DTIC Science & Technology

1983-06-22

OUTPUTS: 16 BIT PROE:UCT IN ACCA , FiCC:B Oi:t2 -* 0011: 0172 B:7 .04E SIFY STA A MCAN:,* SF,-’E MULTIPLICAND 001t4 0175 iF CLF: A INIT RESUI_I_T AOll5...OL-:t41) 04: ELSE ACCA IS UNC:HANGED 0-142 01SF ::,A 0CI CLAMP 0’Fi A #0 TEST SIGN OF A C0It 0l91 E’E: fBM I C.LIPI EBRFNCH IF NEG 01t4 4 : : 001,15 19...ELSE S.:ET ACCA =-ACCB:1B TQJ5. 01 .’ NEG B .ESTOPE ACCE: 01155 C. l1’-9 E -9 PTI RETUR’N Al? I .9~~ _________ . .... ... | |- - - - . . rjOt :E

The Employer's Voice: Frontline Workers and Workforce Development.

ERIC Educational Resources Information Center

Jobs for the Future, Boston, MA.

Seventeen small and mid-sized employers from Annie E. Casey Foundation Jobs Initiative sites addressed the challenges of recruiting, retaining, and promoting frontline workers. Employers shared collaboration experiences with Jobs Initiatives sites to develop effective, efficient strategies to prepare and support low-income residents. Firms relied…

Mentoring in nursing: a historical approach.

PubMed

Fields, W L

1991-01-01

Most nurses today have or have had mentors. Several historical nurse leaders also had mentors. Florence Nightingale's mentor gave her the opportunity to work as a nurse during the Crimean War. Linda Richards, Mary Adelaide Nutting, and Annie Goodrich were all encouraged by their respective mentors to develop professionally.

Abstracts of ARI Research Publications, FY 1976

DTIC Science & Technology

1979-11-01

Personnel FRANKLIN A. HART JOSEPH ZEIDNER Colonel, US Anny Technical Pirector Commander NOTICES DISTRIVUIMON PI*fV d1It.t$0m. ofW this reort h" bfan...University of Washington Salt Lake City, Utah 84112 The Library Documents LibrarianMr. Karlo K. Mustonen Seattle, Washington 98105 Documents Department

Satire as Persuasion.

ERIC Educational Resources Information Center

Gruner, Charles R.

Satire is a genre long extant if not especially beloved in human history. Practitioners of the art claim the intent to persuade and educate through their works. Many quantitative studies have tested the persuasive effects of satire. In research on persuasion, A.D. Annis (1939) compared the effects of editorials and editorial cartoons and concluded…

Money Matters: No It Doesn't, Yes It Does.

ERIC Educational Resources Information Center

Bracey, Gerald W.

1997-01-01

Eric Hanushek's conclusion that money does not matter in public education is debatable. His latest meta-analysis reviews many articles from economics journals, while omitting several published in education journals. He finds no relationship between test results and spending, yet labels schools inefficient. In other research, the Annie Casey…

50th Anniversary First American to Orbit Earth

NASA Image and Video Library

2012-02-20

Sen. John Glenn and his wife Annie listen to speakers during a reception at Ohio State University honoring the 50th anniversary of John Glenn's historic flight aboard Friendship 7 Monday, Feb. 20, 2012, in Columbus, Ohio. Glenn was the first American to orbit Earth. Photo Credit: (NASA/Bill Ingalls)

50th Anniversary First American to Orbit Earth

NASA Image and Video Library

2012-02-20

Sen. John Glenn and his wife Annie talk during a celebration dinner at The Ohio State University honoring the 50th anniversary of John Glenn's historic flight aboard Friendship 7 Monday, Feb. 20, 2012, in Columbus, Ohio. Glenn was the first American to orbit Earth. Photo Credit: (NASA/Bill Ingalls)

Confessions from the Performatively Confused

ERIC Educational Resources Information Center

Williams, Rose

2007-01-01

"Confessions" begins with an auto-ethnographic account of my learning-through-movement in a relationship that was intimate, therapeutic, embodied and instructive--with a teacher called Annie. It seems sensible to start with a choreographic teacher of Feldenkrais therapies and theatre-movement to think about the meanings I import from my roles as…

Adult Communication and Teen Sex: Changing a Community.

ERIC Educational Resources Information Center

Grossman, Jean Baldwin; Walker, Karen E.; Kotloff, Lauren J.; Pepper, Sarah

The Plain Talk initiative, developed by the Annie E. Casey Foundation, was designed to increase the amount and quality of communication that parents and community adults provided youth regarding responsible sexual decision making. It offered parents and other community adults information and skills necessary to communicate more effectively with…

75 FR 42446 - Ocean Transportation Intermediary License Applicants

Federal Register 2010, 2011, 2012, 2013, 2014

2010-07-21

... Change. CACC Global Logistics, Inc. (NVO & OFF), 151 E. 220th Street, Carson, CA 90754. Officers: Annie... NVO & OFF License. E-Freight Solutions Inc. dba E-Lines Shipping and Logistics, and Ocean Champ Shipping Limited (NVO), 1000 Corporate Center Drive, Suite 320, Monterey Park, CA 91754. Officers: Joey Tam...

Holden and Company: A Separate Piece (of the Curriculum).

ERIC Educational Resources Information Center

Brown, Joanne

An instructor of an adolescent literature course wanted to give the students an opportunity to study some novels not specifically written for an adolescent audience. Examples of such novels were: Harper Lee's "To Kill a Mockingbird," J.D. Salinger's "Catcher in the Rye," and Jamaica Kincaid's "Annie John." Including…

AdvoCasey: Documenting Programs That Work for Kids and Families, 2000.

ERIC Educational Resources Information Center

Nelson, Douglas W.; Rust, Bill; Hinds, Michael DeCourcy

2000-01-01

This publication includes five articles on health care and health promotion within impoverished communities. "The Health of Families and Communities" (Douglas W. Nelson) introduces the issue, discussing the work of the Annie E. Casey Foundation in communities. "Inconspicuous Consumption: Treating Latent TB Infection in Seattle"…

Doing School Choice Right: Preliminary Findings

ERIC Educational Resources Information Center

Harvey, James; Hill, Paul T.

2006-01-01

The Center on Reinventing Public Education (CRPE) has launched a new initiative entitled "Doing School Choice Right." Funded by the Bill and Melinda Gates, Annie E. Casey, and Lynde and Harry Bradley Foundations, the initiative's goal is to help state and local leaders handle practical issues whose resolution can determine whether school…

Mental Health Practice Guidelines for Child Welfare

ERIC Educational Resources Information Center

Annie E. Casey Foundation, 2009

2009-01-01

The guidelines and supporting rationale presented in this paper were developed from the October 2007 "Best Practices for Mental Health in Child Welfare Consensus Conference" sponsored by Casey Family Programs, the Annie E. Casey Foundation and the REACH Institute (REsource for Advancing Children's Health). The purpose of the conference was to…

Stories of Change.

ERIC Educational Resources Information Center

Stolz, Paul

In Annie Proulx's novel "The Shipping News," the anti-hero undertakes a journey of change that transforms the way he sees himself and his ways of acting and relating. This novel about the complexity of life and difficulty of change mirrors the course of wilderness-enhanced narrative therapy. Narrative therapy suggests that the sum of…

Langerin negative dendritic cells promote potent CD8+ T-cell priming by skin delivery of live adenovirus vaccine microneedle arrays.

PubMed

Bachy, Veronique; Hervouet, Catherine; Becker, Pablo D; Chorro, Laurent; Carlin, Leo M; Herath, Shanthi; Papagatsias, Timos; Barbaroux, Jean-Baptiste; Oh, Sea-Jin; Benlahrech, Adel; Athanasopoulos, Takis; Dickson, George; Patterson, Steven; Kwon, Sung-Yun; Geissmann, Frederic; Klavinskis, Linda S

2013-02-19

Stabilization of virus protein structure and nucleic acid integrity is challenging yet essential to preserve the transcriptional competence of live recombinant viral vaccine vectors in the absence of a cold chain. When coupled with needle-free skin delivery, such a platform would address an unmet need in global vaccine coverage against HIV and other global pathogens. Herein, we show that a simple dissolvable microneedle array (MA) delivery system preserves the immunogenicity of vaccines encoded by live recombinant human adenovirus type 5 (rAdHu5). Specifically, dried rAdHu5 MA immunization induced CD8(+) T-cell expansion and multifunctional cytokine responses equipotent with conventional injectable routes of immunization. Intravital imaging demonstrated MA cargo distributed both in the epidermis and dermis, with acquisition by CD11c(+) dendritic cells (DCs) in the dermis. The MA immunizing properties were attributable to CD11c(+) MHCII(hi) CD8α(neg) epithelial cell adhesion molecule (EpCAM(neg)) CD11b(+) langerin (Lang; CD207)(neg) DCs, but neither Langerhans cells nor Lang(+) DCs were required for CD8(+) T-cell priming. This study demonstrates an important technical advance for viral vaccine vectors progressing to the clinic and provides insights into the mechanism of CD8(+) T-cell priming by live rAdHu5 MAs.

Nahal Ein Gev II, a Late Natufian Community at the Sea of Galilee

PubMed Central

Grosman, Leore; Munro, Natalie D.; Abadi, Itay; Boaretto, Elisabetta; Shaham, Dana; Belfer-Cohen, Anna; Bar-Yosef, Ofer

2016-01-01

The Natufian culture is of great importance as a starting point to investigate the dynamics of the transition to agriculture. Given its chronological position at the threshold of the Neolithic (ca. 12,000 years ago) and its geographic setting in the productive Jordan Valley, the site of Nahal Ein Gev II (NEG II) reveals aspects of the Late Natufian adaptations and its implications for the transition to agriculture. The size of the site, the thick archaeological deposits, invested architecture and multiple occupation sub-phases reveal a large, sedentary community at least on par with Early Natufian camps in the Mediterranean zone. Although the NEG II lithic tool kit completely lacks attributes typical of succeeding Pre Pottery Neolithic A (PPNA) assemblages, the artistic style is more closely related to the early PPNA world, despite clear roots in Early Natufian tradition. The site does not conform to current perceptions of the Late Natufians as a largely mobile population coping with reduced resource productivity caused by the Younger Dryas. Instead, the faunal and architectural data suggest that the sedentary populations of the Early Natufian did not revert back to a nomadic way of life in the Late Natufian in the Jordan Valley. NEG II encapsulates cultural characteristics typical of both Natufian and PPNA traditions and thus bridges the crossroads between Late Paleolithic foragers and Neolithic farmers. PMID:26815363

[Initial experiences with primiparous women using a new kind of Epi-no labor trainer].

PubMed

Hillebrenner, J; Wagenpfeil, S; Schuchardt, R; Schelling, M; Schneider, K T

2001-01-01

The effectiveness of a vaginal dilatator (Epi-no) in avoiding episiotomies and improving the fetal outcome was examined. Fifty pregnant women were included in our prospective study and took part in the prepartal birth training program with Epi-no. Matched-pairs were compared for the rate of episiotomy and perineal tears, neonatal APGAR score, average time of training, duration of labour and analgesia during delivery. We found a significant reduction in the rate of episiotomies in the group of women who participated in the birth training program with Epi-no (EG: 49%) compared to women who did not take part in our training program (NEG: 82%). Also the rate of perineal tears was twice as high in the latter (4% vs. 2%). Moreover, children of women of the EG showed better one-minute-APGAR-scores. In addition to this we found a significant reduction in the average duration of the second stage of labour in the EG (29 min) if compared with the NEG (54 min). Women in the EG had a lower rate of PDA (16% vs. 36%) and needed less analgesics than those in the NEG. Women of the EG who delivered without episiotomy had trained on average two days longer than women who had had an episiotomy. Birth training with Epi-no decreases the rate of episiotomies in primiparous significantly.

Mindfulness and Self-compassion as Unique and Common Predictors of Affect in the General Population.

PubMed

López, Angélica; Sanderman, Robbert; Schroevers, Maya J

2016-01-01

In contrast to the increased research interest in the benefits of mindfulness and self-compassion, relatively few studies have examined their unique and combined effects in predicting affect. This cross-sectional study examined the predictive value of mindfulness and self-compassion for depressive symptoms, negative affect, and positive affect in a large representative sample of community adults ( N  = 1736). The Five Facets of Mindfulness Questionnaire (FFMQ) was used as a measure of mindfulness and the Self-Compassion Scale (SCS) as a measure of self-compassion. Five FFMQ facets were explored: observe, describe, act with awareness, non-judgment, and non-reactivity. Two SCS facets were explored: its positive items (SCS Pos) and its negative items (SCS Neg). When simultaneously examining all seven facets of mindfulness and self-compassion, three of the five FFMQ facets and SCS Neg significantly predicted both depressive symptoms and negative affect, with SCS Neg and act with awareness being the strongest predictors. These findings suggest that a harsh attitude towards oneself and a lack of attention when acting have the greatest value in predicting the presence of psychological symptoms. With respect to positive affect, four of the five FFMQ facets (except non-judgment) were significant predictors, with no unique predictive value of the two SCS's facets, suggesting that mindfulness is a more important predictor of positive affect than self-compassion, as measured by the FFMQ and SCS.

Nahal Ein Gev II, a Late Natufian Community at the Sea of Galilee.

PubMed

Grosman, Leore; Munro, Natalie D; Abadi, Itay; Boaretto, Elisabetta; Shaham, Dana; Belfer-Cohen, Anna; Bar-Yosef, Ofer

2016-01-01

The Natufian culture is of great importance as a starting point to investigate the dynamics of the transition to agriculture. Given its chronological position at the threshold of the Neolithic (ca. 12,000 years ago) and its geographic setting in the productive Jordan Valley, the site of Nahal Ein Gev II (NEG II) reveals aspects of the Late Natufian adaptations and its implications for the transition to agriculture. The size of the site, the thick archaeological deposits, invested architecture and multiple occupation sub-phases reveal a large, sedentary community at least on par with Early Natufian camps in the Mediterranean zone. Although the NEG II lithic tool kit completely lacks attributes typical of succeeding Pre Pottery Neolithic A (PPNA) assemblages, the artistic style is more closely related to the early PPNA world, despite clear roots in Early Natufian tradition. The site does not conform to current perceptions of the Late Natufians as a largely mobile population coping with reduced resource productivity caused by the Younger Dryas. Instead, the faunal and architectural data suggest that the sedentary populations of the Early Natufian did not revert back to a nomadic way of life in the Late Natufian in the Jordan Valley. NEG II encapsulates cultural characteristics typical of both Natufian and PPNA traditions and thus bridges the crossroads between Late Paleolithic foragers and Neolithic farmers.

A continuous process for the development of Kodak Aerochrome Infrared Film 2443 as a negative

NASA Astrophysics Data System (ADS)

Klimes, D.; Ross, D. I.

1993-02-01

A process for the continuous dry-to-dry development of Kodak Aerochrome Infrared Film 2443 as a negative (CIR-neg) is described. The process is well suited for production processing of long film lengths. Chemicals from three commercial film processes are used with modifications. Sensitometric procedures are recommended for the monitoring of processing quality control. Sensitometric data and operational aerial exposures indicate that films developed in this process have approximately the same effective aerial film speed as films processed in the reversal process recommended by the manufacturer (Kodak EA-5). The CIR-neg process is useful when aerial photography is acquired for resources management applications which require print reproductions. Originals can be readily reproduced using conventional production equipment (electronic dodging) in black and white or color (color compensation).

Negative-Pressure Hydrocephalus: A Case Report on Successful Treatment Under Intracranial Pressure Monitoring with Bilateral Ventriculoperitoneal Shunts.

PubMed

Pandey, Sajan; Jin, Yi; Gao, Liang; Zhou, Cheng Cheng; Cui, Da Ming

2017-03-01

Negative-pressure hydrocephalus (NegPH), a very rare condition of unknown etiology and optimal treatment, usually presents postneurosurgery with clinical and imaging features of hydrocephalus, but with negative cerebrospinal fluid pressure. We describe a NegPH case of -3 mm Hg intracranial pressure that was successfully treated to achieve 5 mm Hg under continuous intracranial pressure monitoring with horizontal positioning, head down and legs elevated to 10°-15°, neck wrapping for controlled venous drainage, chest and abdomen bandages, infusion of 5% dextrose fluid to lower plasma osmolarity (Na + , 130-135 mmol/L), daily cerebrospinal fluid drainage >200 mL, and arterial blood gas partial pressure of carbon dioxide >40 mm Hg. Copyright © 2016 Elsevier Inc. All rights reserved.

Practice guidelines on the use of esophageal manometry - A GISMAD-SIGE-AIGO medical position statement.

PubMed

Savarino, Edoardo; de Bortoli, Nicola; Bellini, Massimo; Galeazzi, Francesca; Ribolsi, Mentore; Salvador, Renato; Savarino, Vincenzo; Penagini, Roberto

2016-10-01

Patients with esophageal symptoms potentially associated to esophageal motor disorders such as dysphagia, chest pain, heartburn and regurgitation, represent one of the most frequent reasons for referral to gastroenterological evaluation. The utility of esophageal manometry in clinical practice is: (1) to accurately define esophageal motor function, (2) to identify abnormal motor function, and (3) to establish a treatment plan based on motor abnormalities. With this in mind, in the last decade, investigations and technical advances, with the introduction of high-resolution esophageal manometry, have enhanced our understanding and management of esophageal motility disorders. The following recommendations were developed to assist physicians in the appropriate use of esophageal manometry in modern patient care. They were discussed and approved after a comprehensive review of the medical literature pertaining to manometric techniques and their recent application. This position statement created under the auspices of the Gruppo Italiano di Studio per la Motilità dell'Apparato Digerente (GISMAD), Società Italiana di Gastroenterologia ed Endoscopia Digestiva (SIGE) and Associazione Italiana Gastroenterologi ed Endoscopisti Digestivi Ospedalieri (AIGO) is intended to help clinicians in applying manometric studies in the most fruitful manner within the context of their patients with esophageal symptoms. Copyright © 2016 Editrice Gastroenterologica Italiana S.r.l. Published by Elsevier Ltd. All rights reserved.

50th Anniversary First American to Orbit Earth

NASA Image and Video Library

2012-02-20

Sen. John Glenn and his wife Annie take the stage during a celebration dinner at The Ohio State University honoring the 50th anniversary of John Glenn's historic flight aboard Friendship 7 Monday, Feb. 20, 2012, in Columbus, Ohio. Glenn was the first American to orbit Earth. Photo Credit: (NASA/Bill Ingalls)

50th Anniversary First American to Orbit Earth

NASA Image and Video Library

2012-02-20

Ohio State University student Joanna Fedeli interviews Sen. John Glenn and his wife Annie during a celebration dinner at Ohio State University honoring the 50th anniversary of John Glenn's historic flight aboard Friendship 7 Monday, Feb. 20, 2012, in Columbus, Ohio. Glenn was the first American to orbit Earth. Photo Credit: (NASA/Bill Ingalls)

50th Anniversary First American to Orbit Earth

NASA Image and Video Library

2012-02-20

Sen. John Glenn's wife Annie listens to an interviewers question during a celebration dinner at The Ohio State University honoring the 50th anniversary of John Glenn's historic flight aboard Friendship 7 Monday, Feb. 20, 2012, in Columbus, Ohio. Glenn was the first American to orbit Earth. Photo Credit: (NASA/Bill Ingalls)

Little Orphan Annie

ERIC Educational Resources Information Center

Hyatt, Sarah

2009-01-01

The benefits of therapeutic riding and hippotherapy can be numerous and long lasting for children and adults with special needs. The connection that a child or adult with disabilities can make with a horse is evident in therapeutic riding programs all over the country. In this article, the author, who is the Equestrian Center Director at Heartland…

Baltimore and the Portfolio School District Strategy. Portfolio School Districts Project

ERIC Educational Resources Information Center

Yatsko, Sarah

2012-01-01

In November 2010, Baltimore's Fund for Education Excellence and the Annie E. Casey Foundation approached the Center on Reinventing Public Education (CRPE) and requested a case study of the implementation of Baltimore City Public Schools' (City Schools) portfolio strategy. These local foundations were interested in understanding how the district…

Great River Environmental Action Team (GREAT II). Upper Mississippi River (Guttenberg, Iowa to Saverton, Missouri) Main Report.

DTIC Science & Technology

1980-12-01

followking studies: * Development studies that show the in- terrelated economic impacts (benefits and costs) that all industries located in tile The...Draitrac District. Anni Arbor Science, Ann Arbrr. Michiganr. Bowie. A. J.. C. C. Bohltot atrd J. A. Spraberr\\ . 19)75. Associated Genteral Contractors of

Paradigm, Persona and Epideictic: The Lovesongs of Eurythmics.

ERIC Educational Resources Information Center

Oglesbee, Frank W.

A study examined the lyrics of the lovesongs of the musical partnership Eurythmics (David Stewart and Annie Lennox), a popular music group. The method used was based on the James Chesbro study, which classified lyrics using a critical paradigm that combined Northrop Frye's hierarchy of communications systems (Ironic, Mimetic, Leader-centered,…

KIDS COUNT Data Book, 2016: State Trends in Child Well-Being

ERIC Educational Resources Information Center

Annie E. Casey Foundation, 2016

2016-01-01

The Annie E. Casey Foundation's "2016 KIDS COUNT Data Book" finds today's youth--Generation Z--are healthier and completing high school on time despite mounting economic inequality and increasingly unaffordable college tuition. Aided by smart policies and investments in prevention, a record number of teens are making positive choices.…

Saving the Last Dance

ERIC Educational Resources Information Center

Douban, Gigi

2007-01-01

This article features a 76-year-old dance program instructor Annie Lindsay. She has been teaching at Ramsay High School in Birmingham, Alabama, since 1970. Maintaining the dance program has become a personal mission for Lindsay. She could have retired long ago, but is afraid that if she does, Birmingham, Alabama's, last public dance school class…

DOE Office of Scientific and Technical Information (OSTI.GOV)

Eisses, A.; Kell, A.; Kent, G.

Amy Eisses, Annie M. Kell, Graham Kent, Neal W. Driscoll, Robert E. Karlin, Robert L. Baskin, John N. Louie, Kenneth D. Smith, Sathish Pullammanappallil, 2011, Marine and land active-source seismic investigation of geothermal potential, tectonic structure, and earthquake hazards in Pyramid Lake, Nevada: presented at American Geophysical Union Fall Meeting, San Francisco, Dec. 5-9, abstract NS14A-08.

Annie's Journey to Healing

ERIC Educational Resources Information Center

Kreisle, Beate

2008-01-01

Ongoing traumatising life events can often cause a person to the have physical or emotional characteristics of one who is mentally disabled or autistic, making it easy to form a wrong first impression. This article presents the story of one such person in a Positive Peer Culture program in a group treatment facility in Germany. In Positive Peer…

Connecting Schools, Families & Communities: Stories and Results from the Annie E. Casey Foundation's Education Investments

ERIC Educational Resources Information Center

Jehl, Jeanne

2007-01-01

Partnerships among schools, families, and community organizations can reconnect urban schools to their communities and improve results for young people and their families. This report provides an overview of Casey's investment philosophy and strategies, and its approach to defining and documenting grantmaking results. The report highlights the…

Mighty Math[TM] Zoo Zillions[TM]. [CD-ROM].

ERIC Educational Resources Information Center

1996

Zoo Zillions contains five activities for grades K-2: Annie's Jungle Trail, 3D Gallery, Number Line Express, Gnu Ewe Boutique, and Fish Stories. These activities enable children to review and practice basic mathematics skills; identify three-dimensional shapes, watch them in motion, and create their own three-dimensional designs; locate numbers…

Neil Armstrong Family Memorial Service

NASA Image and Video Library

2012-08-31

Sen. John Glenn and his wife Annie listen during a memorial service celebrating the life of Neil Armstrong, Friday, Aug. 31, 2012, at the Camargo Club in Cincinnati. Armstrong, the first man to walk on the moon during the 1969 Apollo 11 mission, died Saturday, Aug. 25. He was 82. Photo Credit: (NASA/Bill Ingalls)

Getting Results: Outcomes Management and the Annie E. Casey Foundations Jobs Initiative.

ERIC Educational Resources Information Center

Giloth, Robert; Phillips, William

The Anne E. Casey Foundation (AECF) funded replications of effective jobs projects to achieve better job placement and retention for low-income, young adults. The six projects funded, collectively called the Jobs Initiative (JI), in Denver, Milwaukee, New Orleans, Philadelphia, Seattle, and St. Louis, used an outcomes framework developed by The…

Lessons Learned. Community Change: Lessons from Making Connections

ERIC Educational Resources Information Center

Annie E. Casey Foundation, 2013

2013-01-01

Making Connections was the Annie E. Casey Foundation's signature place-based, community-change initiative of the 2000s. It sought to build on previous work and launch an effort focused firmly on the framework of family strengthening. The Foundation started Making Connections in 22 places, focusing eventually on first 10, then seven sites. It…

Neuroglial heterotopia of the scalp.

PubMed

Attafi, S; Lahmar-Boufaroua, A; Rekik, W; Fraoua, F; Fadhel, C B; Bouraoui, S; Mzabi-Rgaya, S

2016-03-01

Heterotopic glial nodules of the scalp are non hereditary congenital malformations composed of mature brain tissue isolated from the cranial cavity. The majority of these lesions are found in the nasal region and occur rarely on the scalp. They are frequently diagnosed in newborn infants. However, they may rarely be found in adults. The pathogenesis of these lesions remains unknown. We describe the case of a temporal scalp nodule in a 50 year-old man. At the time of the excision, the mass was not associated with intracranial connection. Histological examination revealed neural tissue staining with S100-protein and the glial fibrillary acidic protein (GFAP). © Copyright Società Italiana di Anatomia Patologica e Citopatologia Diagnostica, Divisione Italiana della International Academy of Pathology.

The expression of cholesterol metabolism genes in monocytes from HIV-infected subjects suggests intracellular cholesterol accumulation.

PubMed

Feeney, Eoin R; McAuley, Nuala; O'Halloran, Jane A; Rock, Clare; Low, Justin; Satchell, Claudette S; Lambert, John S; Sheehan, Gerald J; Mallon, Patrick W G

2013-02-15

Human immunodeficiency virus (HIV) infection is associated with increased cardiovascular risk and reduced high-density lipoprotein cholesterol (HDL-c). In vitro, HIV impairs monocyte-macrophage cholesterol efflux, a major determinant of circulating HDL-c, by increasing ABCA1 degradation, with compensatory upregulation of ABCA1 messenger RNA (mRNA). We examined expression of genes involved in cholesterol uptake, metabolism, and efflux in monocytes from 22 HIV-positive subjects on antiretroviral therapy (ART-Treated), 30 untreated HIV-positive subjects (ART-Naive), and 22 HIV-negative controls (HIV-Neg). HDL-c was lower and expression of ABCA1 mRNA was higher in ART-Naive subjects than in both ART-Treated and HIV-Neg subjects (both P < .01), with HDL-c inversely correlated with HIV RNA (ρ = -0.52; P < .01). Expression of genes involved in cholesterol uptake (LDLR, CD36), synthesis (HMGCR), and regulation (SREBP2, LXRA) was significantly lower in both ART-Treated and ART-Naive subjects than in HIV-Neg controls. In vivo, increased monocyte ABCA1 expression in untreated HIV-infected patients and normalization of ABCA1 expression with virological suppression by ART supports direct HIV-induced impairment of cholesterol efflux previously demonstrated in vitro. However, decreased expression of cholesterol sensing, uptake, and synthesis genes in both untreated and treated HIV infection suggests that both HIV and ART affect monocyte cholesterol metabolism in a pattern consistent with accumulation of intramonocyte cholesterol.

Cardiac Damage Biomarkers Following a Triathlon in Elite and Non-elite Triathletes

PubMed Central

Park, Chan-Ho; Kim, Kwi-Baek; Han, Jin; Ji, Jin-Goo

2014-01-01

The purpose of the present study was to investigate cardiac damage biomarkers after a triathlon race in elite and non-elite athlete groups. Fifteen healthy men participated in the study. Based on performance, they were divided into elite athlete group (EG: n=7) and non-elite athlete group (NEG: n=8). Participants' blood samples were obtained during four periods: before, immediately, 2 hours and 7 days after finishing the race. creatine kinase (CK), creatine kinase-myoglobin (CK-MB), myoglobin, and lactate dehydrogenase (LDH) were significantly increased in both groups immediately after, and 2 hours after finishing the race (p<.05). CK, CK-MB, and myoglobin were completely recovered after 7 days (p<.05). Hematocrit (Hct) was significantly decreased in both groups (p<.05) 7 days after the race. LDH was significantly decreased in the EG (p<.05) only 7 days after the race. Homoglobin (Hb) was significantly decreased in the NEG (p<.05) only 2 hours after the race. Although cardiac troponin T (cTnT) was significantly increased in the EG but not in the NEG 2hours after the race (p<.05), there was no group-by-time interaction. cTnT was completely recovered in both groups 7 days after the race. In conclusion, cardiac damage occurs during a triathlon race and, is greater in elite than in non-elite. However, all cardiac damage markers return to normal range within 1 week. PMID:25352762

Enrichment, Isolation and Molecular Characterization of EpCAM-Negative Circulating Tumor Cells.

PubMed

Lampignano, Rita; Schneck, Helen; Neumann, Martin; Fehm, Tanja; Neubauer, Hans

2017-01-01

The presence of EpCAM-positive circulating tumor cells (CTCs) in the peripheral blood is associated with poor clinical outcomes in breast, colorectal and prostate cancer, as well as the prognosis of other tumor types. In addition, recent studies have suggested that the presence of CTCs undergoing epithelial-to-mesenchymal transition and, as such, may exhibit reduced or no expression of epithelial proteins e.g. EpCAM, might be related to disease progression in metastatic breast cancer (MBC) patients. Analyzing the neoplastic nature of this EpCAM-low/negative (EpCAM-neg) subpopulation remains an open issue as the current standard detection methods for CTCs are not efficient at identifying this subpopulation of cells. The possible association of EpCAM-neg CTCs with EpCAM-positive (EpCAM-pos) CTCs and role in the clinicopathological features and prognosis of MBC patients has still to be demonstrated. Several technologies have been developed and are currently being tested for the identification and the downstream analyses of EpCAM-pos CTCs. These technologies can be adapted and implemented into workflows to isolate and investigate EpCAM-neg cells to understand their biology and clinical relevance. This chapter will endeavour to explain the rationale behind the identification and analyses of all CTC subgroups, as well as to review the current strategies employed to enrich, isolate and characterize EpCAM-negative CTCs. Finally, the latest findings in the field will briefly be discussed with regard to their clinical relevance.

A study of a sector spectrophotometer and auroral O+(2P-2D) emissions

NASA Technical Reports Server (NTRS)

Swenson, G. R.

1976-01-01

The metastable O+(2P-2D) auroral emission was investigated. The neighboring OH contaminants and low intensity levels of the emission itself necessitated the evolution of an instrument capable of separating the emission from the contaminants and having a high sensitivity in the wavelength region of interest. A new type of scanning photometer was developed and its properties are discussed. The theoretical aspects of auroral electron interaction with atomic oxygen and the resultant O+(2P-2D) emissions were examined in conjunction with N2(+)1NEG emissions. Ground based measurements of O+(2P-2D) auroral emission intensities were made using the spatial scanning photometer (sector spectrophotometer). Simultaneous measurements of N2(+)1NEG sub 1,0 emission intensity were made in the same field of view using a tilting photometer. Time histories of the ratio of these two emissions made in the magnetic zenith during auroral breakup periods are given. Theories of I sub 7319/I sub 4278 of previous investigators were presented. A rocket measurement of N2(+)1NEG sub 0,0 and O+(2P-2D) emission in aurora was examined in detail and was found to agree with the ground based measurements. Theoretical examination resulted in the deduction of the electron impact efficiency generating O+(2P) and also suggests a large source of O+(2P) at low altitude. A possible source is charge exchange of N+(1S) with OI(3P).

Acknowledgements

NASA Astrophysics Data System (ADS)

2016-07-01

The Meeting was sponsored by the Istituto Nazionale di Fisica Nucleare (INFN), the Società Italiana di Fisica (SIF), the European Physics Society (EPS), the University of Pisa and the University of Siena.

Small Ships in Theater Security Cooperation

DTIC Science & Technology

2008-01-01

ENVIRONMENT HEALTH AND HEALTH CARE INTERNATIONAL AFFAIRS NATIONAL SECURITY POPULATION AND AGING PUBLIC SAFETY SCIENCE AND TECHNOLOGY SUBSTANCE ABUSE...Civil Affairs, Washington, D.C.: Joint Staff, April14, 2003. Marquis, Jefferson P., Richard E. Darilek, Jasen J. Castillo, Cathryn Quantic Thurston...McFadden, Cathryn Quantic Thurston, and Anny Wong. Building Partner Capabilities for Coalition Operations, Santa Monica, Calif.: RAND Corporation, MG

County Data for Community Action: 1996 Status of Oregon's Children.

ERIC Educational Resources Information Center

Oregon State Dept. of Human Resources, Salem.

This Children First for Oregon report, funded by a Kids Count grant from the Annie Casey Foundation, investigates state and countywide trends in the well-being of Oregon's children. The statistical report is based on 14 indicators of child well-being: (1) child abuse and neglect rates; (2) crimes against persons; (3) child death rate; (4) prenatal…

Finding Funding: Supporting Making Connections Core Result that Children are Healthy and Prepared to Succeed in School

ERIC Educational Resources Information Center

Lind, Christianne; Crocker, Jarle; Stewart, Nichole; Torrico, Roxana; Bhat, Soumya; Schmid, William; Ennis, Melissa, Ed.

2009-01-01

The Annie E. Casey Foundation's "Making Connections" initiative provides support to communities across the country to help improve outcomes for children and families in disadvantaged neighborhoods. The sites, together with local partners, are engaged in a range of strategies and activities, reflecting local conditions and priorities, to ensure…

50th Anniversary First American to Orbit Earth

NASA Image and Video Library

2012-02-20

Sen. John Glenn's wife Annie, right, and NASA Deputy Administrator Lori Garver pose for a photograph during a celebration dinner at Ohio State University honoring the 50th anniversary of John Glenn's historic flight aboard Friendship 7 Monday, Feb. 20, 2012, in Columbus, Ohio. Glenn was the first American to orbit Earth. Photo Credit: (NASA/Bill Ingalls)

Who Can Be a Hero?: Helen Keller, Annie Sullivan, and Discovering Strength of Character

ERIC Educational Resources Information Center

Morin, Kathleen Dunlevy; Bernheim, Rachel Oestreicher

2005-01-01

"A Study of Heroes: Making a Difference Using Your Heart, Intellect, and Talents" is a program originally developed in diverse school communities. Students learn to distinguish between the concepts of hero and celebrity and to discover the real heroes in their own families, schools, communities, and most importantly--within themselves. This…

Taking the Initiative on Jobs & Race: Innovations in Workforce Development for Minority Job Seekers and Employers.

ERIC Educational Resources Information Center

Annie E. Casey Foundation, Baltimore, MD.

The Annie E. Casey Foundation launched the eight-year, six-city demonstration project, Jobs Initiative (JI), in 1995 to provide support and assistance to community groups, employers, foundations, and community colleges helping disadvantaged, low-skilled workers secure family-supporting jobs. JI sites found that even during a time when employers…

Never Good Enough: The Educational Journey of a Vietnamese American Woman

ERIC Educational Resources Information Center

Nguyen, Annie T.

2014-01-01

In this article, author Annie Nguyen describes her personal encounters with the "Model Minority Myth" as a young Vietnamese American in a doctoral program. This myth assumes that all Asian Americans are inherently smart and high achieving, which is problematic when in fact there are many Asian American individuals who perform poorly.…

Operation Dragoon: The Race Up the Rhone

DTIC Science & Technology

2016-05-26

should be familiar with the exploits of the US Seventh Anny and its French allies. 1S. SUBJECT TERMS Operation Dragoon, European Theater of...the Rhone Valley, and its overall effects on the European Theater of Operations, useful lessons are drawn for both contemporary commanders and planners...57 Southern France’s Effects on the European Theater of Operations

Complex Personhood as the Context for Intimate Partner Victimization: One American Indian Woman's Story

ERIC Educational Resources Information Center

Murphy, Sharon; Lemire, Lynne; Wisman, Mindi

2009-01-01

This qualitative case study explores one American Indian (AI) woman's experience of intimate partner violence and the subsequent murder of her abusive partner. The lens of complex personhood (Gordon, 1997) has been applied as a method for understanding "Annie's" multiple identities of AI woman, victim of intimate partner violence, mother, and…

Kids Count Data Book, 2012: State Trends in Child Well-Being

ERIC Educational Resources Information Center

Annie E. Casey Foundation, 2012

2012-01-01

The Annie E. Casey Foundation's 2012 KIDS COUNT[R] Data Book shows both promising progress and discouraging setbacks for the nation's children: While their academic achievement and health improved in most states, their economic well-being continued to decline. This year's Data Book uses an updated index of 16 indicators of child well-being,…

Youth Count: Exploring How KIDS COUNT Grantees Address Youth Issues

ERIC Educational Resources Information Center

Wilson-Ahlstrom, Alicia; Gaines, Elizabeth; Ferber, Thaddeus; Yohalem, Nicole

2005-01-01

Inspired by the 2004 Kids Count Databook essay, "Moving Youth From Risk to Opportunity," this new report highlights the history of data collection, challenges and innovative strategies of 12 Annie E. Casey Foundation KIDS COUNT grantees in their work to serve the needs of older youth. (Contains 3 figures, 2 tables, and 9 notes.)

Musical Literacies in the English Inter-War Secondary-School Classroom

ERIC Educational Resources Information Center

Goodman, Joyce; Jacobs, Andrea

2008-01-01

This article looks at ways in which the notion of music as a language with a literature operated in English girls' secondary education in the inter-war years. It explores musical literacies and multi-modality in the music curriculum of two inter-war music teachers working in girls' schools: Margaret Donington and Annie Warburton. Both contributed…

Race for Results: Building a Path to Opportunity for All Children

ERIC Educational Resources Information Center

Annie E. Casey Foundation, 2014

2014-01-01

In this policy report, the Annie E. Casey Foundation explores the intersection of kids, race, and opportunity. The report features the new Race for Results index, which compares how children are progressing on key milestones across racial and ethnic groups at the national and state level. The index is based on 12 indicators that measure a…

78 FR 25739 - Combined Notice of Filings

Federal Register 2010, 2011, 2012, 2013, 2014

2013-05-02

...: Transcontinental Gas Pipe Line Company. Description: Annual Adjustment to Rate Schedule SS-2 Storage Gas Balances.... Description: Removal of Non Conforming and Neg Rate TSAs and Points of Contact Update Filing to be effective 5...

Analyzing traffic layout using dynamic social network analysis.

DOT National Transportation Integrated Search

2014-07-12

it is essential to build, maintain, and use our transportation systems in a manner that meets our current : needs while addressing the social and economic needs of future generations. In todays world, : transportation congestion causes serious neg...

Assessing regulatory emotional self-efficacy in three countries.

PubMed

Caprara, Gian Vittorio; Di Giunta, Laura; Eisenberg, Nancy; Gerbino, Maria; Pastorelli, Concetta; Tramontano, Carlo

2008-09-01

The Regulatory Emotional Self-Efficacy (RESE) scale was developed to assess perceived self-efficacy in managing negative (NEG) and in expressing positive (POS) affect (G. V. Caprara & M. Gerbino, 2001). In this study of young adults, the factorial structure of the RESE scale was found to be similar in Italy, the United States, and Bolivia. In addition to a factor for POS, NEG was represented by a second-order factor of 2 different negative affects: despondency-distress (DES) and anger-irritation (ANG). Overall, there was partial invariance at both metric and scalar levels across gender and countries. Discriminant and convergent validity of the RESE scale was further examined in the Italian sample. Stronger patterns of association of POS with prosocial behavior, of ANG with low aggressive behavior problems and irritability, and of DES with low anxiety/depressive problems and shyness and high self-esteem were found. (c) 2008 APA, all rights reserved.

Ionization-Assisted Getter Pumping for Ultra-Stable Trapped Ion Frequency Standards

NASA Technical Reports Server (NTRS)

Tjoelker, Robert L.; Burt, Eric A.

2010-01-01

A method eliminates (or recovers from) residual methane buildup in getter-pumped atomic frequency standard systems by applying ionizing assistance. Ultra-high stability trapped ion frequency standards for applications requiring very high reliability, and/or low power and mass (both for ground-based and space-based platforms) benefit from using sealed vacuum systems. These systems require careful material selection and system processing (cleaning and high-temperature bake-out). Even under the most careful preparation, residual hydrogen outgassing from vacuum chamber walls typically limits the base pressure. Non-evaporable getter pumps (NEGs) provide a convenient pumping option for sealed systems because of low mass and volume, and no power once activated. An ion gauge in conjunction with a NEG can be used to provide a low mass, low-power method for avoiding the deleterious effects of methane buildup in high-performance frequency standard vacuum systems.

Depopulation of Caged Layer Hens with a Compressed Air Foam System.

PubMed

Gurung, Shailesh; Hoffman, John; Stringfellow, Kendre; Abi-Ghanem, Daad; Zhao, Dan; Caldwell, David; Lee, Jason; Styles, Darrel; Berghman, Luc; Byrd, James; Farnell, Yuhua; Archer, Gregory; Farnell, Morgan

2018-01-11

During the 2014-2015 US highly pathogenic avian influenza (HPAI) outbreak, 50.4 million commercial layers and turkeys were affected, resulting in economic losses of $3.3 billion. Rapid depopulation of infected poultry is vital to contain and eradicate reportable diseases like HPAI. The hypothesis of the experiment was that a compressed air foam (CAF) system may be used as an alternative to carbon dioxide (CO₂) inhalation for depopulating caged layer hens. The objective of this study was to evaluate corticosterone (CORT) and time to cessation of movement (COM) of hens subjected to CAF, CO₂ inhalation, and negative control (NEG) treatments. In Experiment 1, two independent trials were conducted using young and spent hens. Experiment 1 consisted of five treatments: NEG, CO₂ added to a chamber, a CO₂ pre-charged chamber, CAF in cages, and CAF in a chamber. In Experiment 2, only spent hens were randomly assigned to three treatments: CAF in cages, CO₂ added to a chamber, and aspirated foam. Serum CORT levels of young hens were not significantly different among the CAF in cages, CAF in a chamber, NEG control, and CO₂ inhalation treatments. However, spent hens subjected to the CAF in a chamber had significantly higher CORT levels than birds in the rest of the treatments. Times to COM of spent hens subjected to CAF in cages and aspirated foam were significantly greater than of birds exposed to the CO₂ in a chamber treatment. These data suggest that applying CAF in cages is a viable alternative for layer hen depopulation during a reportable disease outbreak.

Functional abdominal pain in childhood and long-term vulnerability to anxiety disorders.

PubMed

Shelby, Grace D; Shirkey, Kezia C; Sherman, Amanda L; Beck, Joy E; Haman, Kirsten; Shears, Angela R; Horst, Sara N; Smith, Craig A; Garber, Judy; Walker, Lynn S

2013-09-01

Cross-sectional studies link functional abdominal pain (FAP) to anxiety and depression in childhood, but no prospective study has evaluated psychiatric status in adulthood or its relation to pain persistence. Pediatric patients with FAP (n = 332) and control subjects (n = 147) were tracked prospectively and evaluated for psychiatric disorders and functional gastrointestinal disorders (FGIDs) at follow-up in adolescence and young adulthood (mean age = 20.01 years). Participants were classified according to presence (FGID-POS) or absence (FGID-NEG) of FGIDs at follow-up. Lifetime and current risk of anxiety disorders was higher in FAP than controls (lifetime: 51% vs 20%; current: 30% vs 12%). Controlling for gender and age, the odds ratio was 4.9 (confidence interval = 2.83-7.43) for lifetime anxiety disorder and 3.57 (confidence interval = 2.00-6.36) for current anxiety disorder at follow-up for FAP versus controls. Lifetime risk of depressive disorder was significantly higher in FAP versus controls (40% vs. 16%); current risk did not differ. In most cases, initial onset of anxiety disorders was before pediatric FAP evaluation; onset of depressive disorders was subsequent to FAP evaluation. Within the FAP group, risk of current anxiety disorders at follow-up was significantly higher for FGID-POS versus FGID-NEG (40% vs 24%), and both were higher than controls (12%); current depressive disorders did not differ across FGID-POS, FGID-NEG, and controls. Patients with FAP carry long-term vulnerability to anxiety that begins in childhood and persists into late adolescence and early adulthood, even if abdominal pain resolves.

CD8(+) T-cell Cytotoxic Capacity Associated with Human Immunodeficiency Virus-1 Control Can Be Mediated through Various Epitopes and Human Leukocyte Antigen Types.

PubMed

Migueles, Stephen A; Mendoza, Daniel; Zimmerman, Matthew G; Martins, Kelly M; Toulmin, Sushila A; Kelly, Elizabeth P; Peterson, Bennett A; Johnson, Sarah A; Galson, Eric; Poropatich, Kate O; Patamawenu, Andy; Imamichi, Hiromi; Ober, Alexander; Rehm, Catherine A; Jones, Sara; Hallahan, Claire W; Follmann, Dean A; Connors, Mark

2015-01-01

Understanding natural immunologic control over Human Immunodeficiency Virus (HIV)-1 replication, as occurs in rare long-term nonprogressors/elite controllers (LTNP/EC), should inform the design of efficacious HIV vaccines and immunotherapies. Durable control in LTNP/EC is likely mediated by highly functional virus-specific CD8(+) T-cells. Protective Human Leukocyte Antigen (HLA) class I alleles, like B*27 and B*57, are present in most, but not all LTNP/EC, providing an opportunity to investigate features shared by their HIV-specific immune responses. To better understand the contribution of epitope targeting and conservation to immune control, we compared the CD8(+) T-cell specificity and function of B*27/57(neg) LTNP/EC (n = 23), B*27/57(pos) LTNP/EC (n = 23) and B*27/57(neg) progressors (n = 13). Fine mapping revealed 11 previously unreported immunodominant responses. Although B*27/57(neg) LTNP/EC did not target more highly conserved epitopes, their CD8(+) T-cell cytotoxic capacity was significantly higher than progressors. Similar to B*27/57(pos) LTNP/EC, this superior cytotoxicity was mediated by preferential expansion of immunodominant responses and lysis through the predicted HLA. These findings suggest that increased CD8(+) T-cell cytotoxic capacity is a common mechanism of control in most LTNP/EC regardless of HLA type. They also suggest that potent cytotoxicity can be mediated through various epitopes and HLA molecules and could, in theory, be induced in most people.

CD8+ T-cell Cytotoxic Capacity Associated with Human Immunodeficiency Virus-1 Control Can Be Mediated through Various Epitopes and Human Leukocyte Antigen Types

PubMed Central

Migueles, Stephen A.; Mendoza, Daniel; Zimmerman, Matthew G.; Martins, Kelly M.; Toulmin, Sushila A.; Kelly, Elizabeth P.; Peterson, Bennett A.; Johnson, Sarah A.; Galson, Eric; Poropatich, Kate O.; Patamawenu, Andy; Imamichi, Hiromi; Ober, Alexander; Rehm, Catherine A.; Jones, Sara; Hallahan, Claire W.; Follmann, Dean A.; Connors, Mark

2014-01-01

Understanding natural immunologic control over Human Immunodeficiency Virus (HIV)-1 replication, as occurs in rare long-term nonprogressors/elite controllers (LTNP/EC), should inform the design of efficacious HIV vaccines and immunotherapies. Durable control in LTNP/EC is likely mediated by highly functional virus-specific CD8+ T-cells. Protective Human Leukocyte Antigen (HLA) class I alleles, like B*27 and B*57, are present in most, but not all LTNP/EC, providing an opportunity to investigate features shared by their HIV-specific immune responses. To better understand the contribution of epitope targeting and conservation to immune control, we compared the CD8+ T-cell specificity and function of B*27/57neg LTNP/EC (n = 23), B*27/57pos LTNP/EC (n = 23) and B*27/57neg progressors (n = 13). Fine mapping revealed 11 previously unreported immunodominant responses. Although B*27/57neg LTNP/EC did not target more highly conserved epitopes, their CD8+ T-cell cytotoxic capacity was significantly higher than progressors. Similar to B*27/57pos LTNP/EC, this superior cytotoxicity was mediated by preferential expansion of immunodominant responses and lysis through the predicted HLA. These findings suggest that increased CD8+ T-cell cytotoxic capacity is a common mechanism of control in most LTNP/EC regardless of HLA type. They also suggest that potent cytotoxicity can be mediated through various epitopes and HLA molecules and could, in theory, be induced in most people. PMID:26137533

Non-enzymatic glycation alters microdamage formation in human cancellous bone⋆

PubMed Central

Tang, S.Y.; Vashishth, D.

2015-01-01

Introduction The accumulation of advanced glycation end-products (AGEs) in bone has been suggested to adversely affect the fracture resistance of bone with aging, diabetes, and pharmacological treatments. The formation of AGEs increases crosslinking in the organic matrix of bone but it is unknown how elevated levels of AGEs affect the mechanisms of fracture resistance such as microdamage formation. Methods Human tibial cancellous bone cores were subjected to non-enzymatic glycation (NEG) by in vitro ribosylation and were mechanically loaded to pre- (0.6%) and post- (1.1%) yield apparent level strains. Loaded specimens were stained with lead–uranyl acetate and subjected to microCT-based 3D quantification and characterization of microdamage as either diffuse damage and linear microcracks. Damaged volume per bone volume (DV/BV) and damaged surface per damaged volume (DS/DV) ratios were used to quantify the volume and morphology of the detected microdamage, respectively. Results In vitro ribosylation increased the microdamage morphology parameter (DS/DV) under both pre-(p<0.05; +51%) and post-yield loading (p<0.001; +38%), indicating that the alteration of bone matrix by NEG caused the formation of crack-like microdamage morphologies. Under post-yield loading, the NEG-mediated increase in DS/DV was coupled with the reductions in microdamage formation (DV/BV; p<0.001) and toughness (p<0.001). Discussion Using a novel microCT technique to characterize and quantify microdamage, this study shows that the accumulation of AGEs in the bone matrix significantly alters the quantity and morphology of microdamage production and results in reduced fracture resistance. PMID:19747573

Effects of parsley (Petroselinum crispum) extract versus glibornuride on the liver of streptozotocin-induced diabetic rats.

PubMed

Ozsoy-Sacan, Ozlem; Yanardag, Refiye; Orak, Haci; Ozgey, Yasemin; Yarat, Aysen; Tunali, Tugba

2006-03-08

Parsley (Petroselinum crispum) is one of the medicinal herbs used by diabetics in Turkey. The aim of this study is to investigate the effects of parsley (2g/kg) and glibornuride (5mg/kg) on the liver tissue of streptozotocin-induced diabetic rats. Swiss albino rats were divided into six groups: control; control+parsley; control+glibornuride; diabetic; diabetic+parsley; diabetic+glibornuride. Diabetes was induced by intraperitoneal injection of 65 mg/kg streptozotocin (STZ). Parsley extract and glibornuride were given daily to both diabetic and control rats separately, until the end of the experiment, at day 42. The drugs were administered to one diabetic and one control group from days 14 to 42. On day 42, liver tissues were taken from each rat. In STZ-diabetic group, blood glucose levels, serum alkaline phosphatase activity, uric acid, sialic acid, sodium and potassium levels, liver lipid peroxidation (LPO), and non-enzymatic glycosylation (NEG) levels increased, while liver glutathione (GSH) levels and body weight decreased. In the diabetic group given parsley, blood glucose, serum alkaline phosphatase activity, sialic acid, uric acid, potassium and sodium levels, and liver LPO and NEG levels decreased, but GSH levels increased. The diabetic group, given glibornuride, blood glucose, serum alkaline phosphatase activity, serum sialic acid, uric acid, potassium, and liver NEG levels decreased, but liver LPO, GSH, serum sodium levels, and body weight increased. It was concluded that probably, due to its antioxidant property, parsley extract has a protective effect comparable to glibornuride against hepatotoxicity caused by diabetes.

New serum biomarkers for prostate cancer diagnosis

PubMed Central

Chadha, Kailash C.; Miller, Austin; Nair, Bindukumar B.; Schwartz, Stanley A.; Trump, Donald L.; Underwood, Willie

2014-01-01

Background Prostate-specific antigen (PSA) is currently used as a biomarker for diagnosis and management of prostate cancer (CaP). However, PSA typically lacks the sensitivity and specificity desired of a diagnostic marker. Objective The goal of this study was to identify an additional biomarker or a panel of biomarkers that is more sensitive and specific than PSA in differentiating benign versus malignant prostate disease and/or localized CaP versus metastatic CaP. Methods Concurrent measurements of circulating interleukin-8 (IL-8), Tumor necrosis factor-α (TNF-α) and soluble tumor necrosis factor-α receptors 1 (sTNFR1) were obtained from four groups of men: (1) Controls (2) with elevated prostate-specific antigen with a negative prostate biopsy (elPSA_negBx) (3) with clinically localized CaP and (4) with castration resistant prostate cancer. Results TNF-α Area under the receiver operating characteristic curve (AUC = 0.93) and sTNFR1 (AUC = 0.97) were strong predictors of elPSA_negBx (vs. CaP). The best predictor of elPSA_negBx vs CaP was sTNFR1 and IL-8 combined (AUC = 0.997). The strongest single predictors of localized versus metastatic CaP were TNF-α (AUC = 0.992) and PSA (AUC = 0.963) levels. Conclusions The specificity and sensitivity of a PSA-based CaP diagnosis can be significantly enhanced by concurrent serum measurements of IL-8, TNF-α and sTNFR1. In view of the concerns about the ability of PSA to distinguish clinically relevant CaP from indolent disease, assessment of these biomarkers in the larger cohort is warranted. PMID:25593898

50th Anniversary First American to Orbit Earth

NASA Image and Video Library

2012-02-20

NASA Administrator Charles Bolden, seated right, and Sen. John Glenn address questions from the press during a briefing at Ohio State University as John Glenn's wife Annie Glenn, seated in red, looks on Monday, Feb. 20, 2012, in Columbus, Ohio. Today marks the 50th anniversary of Glenn's historic flight. Glenn was the first American to orbit Earth. Photo Credit: (NASA/Bill Ingalls)

Building Partner Capabilities for Coalition Operations

DTIC Science & Technology

2007-01-01

HEALTH CARE INTERNATIONAL AFFAIRS NATIONAL SECURITY POPULATION AND AGING PUBLIC SAFETY SCIENCE AND TECHNOLOGY SUBSTANCE ABUSE TERRORISM AND HOMELAND...distribution unlimited Jennifer D. P. Moroney • Nancy E. Blacker Renee Buhr • James McFadden Cathryn Quantic Thurston • Anny Wong The RAND...4211-8 (pbk. : alk. paper) 1. Combined operations (Military science ) 2. United States. Army— Organization. 3. Multinational armed forces—Organization

Putting the "Development" in Professional Development: Understanding and Overturning Educational Leaders' Immunities to Change

ERIC Educational Resources Information Center

Helsing, Deborah; Howell, Annie; Kegan, Robert; Lahey, Lisa

2008-01-01

In this article, authors Deborah Helsing, Annie Howell, Robert Kegan, and Lisa Lahey argue that today's educational leaders face a host of complex demands as they strive to implement lasting, meaningful change in their school environments. As these demands often require a level of personal development many adults may not yet have, there is a need…

"[A]s if She Defied the World in her Joyousness": Rereading Darwin on Emotion and Emotional Development

ERIC Educational Resources Information Center

Conrad, Rachel

2004-01-01

Darwin's ideas about emotion are known from his published scientific writings [e.g., Darwin, 1872/1998, 1877], which focus primarily on the evolutionary origins of emotional expressions. The present paper offers an analysis of a personal document--Darwin's memorial of his daughter Annie, who died at age 10 [Darwin, 1989]--which reveals additional…

KIDS COUNT Data Book, 2006. State Profiles of Child Well-Being Series

ERIC Educational Resources Information Center

Annie E. Casey Foundation, 2006

2006-01-01

This national and state-by-state study reports on the well-being of America's children and promotes discussion on ways to secure better futures for all kids. The Data Book ranks states on 10 key indicators and provides information on child health, education, and family economic conditions. This year, the Annie E. Casey Foundation also explores how…

On Paying Attention: Flagpoles, Mindfulness, and Teaching Writing

ERIC Educational Resources Information Center

Kroll, Keith

2008-01-01

To pay attention--to observe, to see the richness and detail that is right before everyone--is the essence of mindfulness. It is also, the author argues, the essence of good writing--the kind of writing for which there is a long American tradition of writers such as Emerson, Thoreau, E. B. White, Barry Lopez, Annie Dillard, Joan Didion, John…

Open to Horror: The Great Plains Situation in Contemporary Thrillers by E. E. Knight and by Douglas Preston and Lincoln Child

ERIC Educational Resources Information Center

Emrys, A. B.

2009-01-01

From the agoraphobic prairie where the father of Willa Cather's Antonia kills himself, to the claustrophobic North Dakota town of Argus devastated by storm in Louise Erdrich's "Fleur," to Lightning Flat, the grim home of Jack Twist in Annie Proulx's "Brokeback Mountain," much Great Plains literature is situational, placing…

4. Historic American Buildings Survey James C. Massey, Photographer 1964 ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

4. Historic American Buildings Survey James C. Massey, Photographer 1964 MAIN (SOUTH) ENTRANCE (4x5' b&w film copy neg. from 35mm slide) - Albert F. Madlener House, 4 West Burton Place, Chicago, Cook County, IL

DOE Office of Scientific and Technical Information (OSTI.GOV)

Rudkevich, Aleksandr; Goldis, Evgeniy

This research conducted by the Newton Energy Group, LLC (NEG) is dedicated to the development of pCloud: a Cloud-based Power Market Simulation Environment. pCloud is offering power industry stakeholders the capability to model electricity markets and is organized around the Software as a Service (SaaS) concept -- a software application delivery model in which software is centrally hosted and provided to many users via the internet. During the Phase I of this project NEG developed a prototype design for pCloud as a SaaS-based commercial service offering, system architecture supporting that design, ensured feasibility of key architecture's elements, formed technological partnershipsmore » and negotiated commercial agreements with partners, conducted market research and other related activities and secured funding for continue development of pCloud between the end of Phase I and beginning of Phase II, if awarded. Based on the results of Phase I activities, NEG has established that the development of a cloud-based power market simulation environment within the Windows Azure platform is technologically feasible, can be accomplished within the budget and timeframe available through the Phase II SBIR award with additional external funding. NEG believes that pCloud has the potential to become a game-changing technology for the modeling and analysis of electricity markets. This potential is due to the following critical advantages of pCloud over its competition: - Standardized access to advanced and proven power market simulators offered by third parties. - Automated parallelization of simulations and dynamic provisioning of computing resources on the cloud. This combination of automation and scalability dramatically reduces turn-around time while offering the capability to increase the number of analyzed scenarios by a factor of 10, 100 or even 1000. - Access to ready-to-use data and to cloud-based resources leading to a reduction in software, hardware, and IT costs. - Competitive pricing structure, which will make high-volume usage of simulation services affordable. - Availability and affordability of high quality power simulators, which presently only large corporate clients can afford, will level the playing field in developing regional energy policies, determining prudent cost recovery mechanisms and assuring just and reasonable rates to consumers. - Users that presently do not have the resources to internally maintain modeling capabilities will now be able to run simulations. This will invite more players into the industry, ultimately leading to more transparent and liquid power markets.« less

[Changes and clinical significance of peripheral blood natural killer cells in neonates with bacterial pneumonia].

PubMed

Li, Qiuling; Weng, Kaizhi; Zhu, Ling; Mei, Xuqiao; Xu, Liping; Lin, Jiehua

2014-10-01

To detect the percentage of total natural killer (NK) cells and its different populations in the peripheral blood from neonates with bacterial pneumonia and discuss the clinical significance of NK cells in the pathogenesis of bacterial pneumonia. Flow cytometry was performed to detect the percentages of NK cells and its subsets in peripheral blood lymphocytes from 38 cases of neonatal bacterial pneumonias and 18 cases of normal neonates. Patients recruited were divided into two groups according to hospitalization days and numbers of peripheral leukocytes: hospitalization days within 10 days (including 10 days) as group A, and more than 10 days as group B; the number of peripheral blood leukocytes <5.0×10(9)/L or >20.0×10(9)/L as severe infection group, and 5.0×10(9)/L< number of peripheral blood leukocytes <20.0×10(9)/L as mild infection group. The percentages of peripheral blood NK cells and CD3(-)CD56(neg)CD16(bright) subset in the neonates with bacterial pneumonia were significantly lower than those of the normal newborns (P<0.01), but there were no statistically significant differences in CD3(-)CD56(bright)CD16(neg/dim) and CD3(-)CD56(dim)CD16(bright) subsets. The percentage of CD3(-)CD56(neg)CD16(bright) subset in group A was significantly lower than that of the normal newborns (P<0.01), while the percentages of the total NK cells and other subsets had no statistical significance. The neonates with bacterial pneumonia had significantly lower percentages of the total NK cells and CD3(-)CD56(neg)CD16(bright) subset in group B as compared with the normal neonates (P<0.01). And the percentages of the total NK cells and its subsets in group B were also lower than those in group A (P<0.05). The percentages of NK cells and each subset in severe infection group were significantly lower than those in mild infection group (P<0.05). To the neonates who suffer from bacterial pneumonia, the more serious and the longer hospital stay, the lower the percentages of NK cells and its subsets are.

Arsenic loads in Spearfish Creek, western South Dakota, water years 1989-91

USGS Publications Warehouse

Driscoll, Daniel G.; Hayes, Timothy S.

1995-01-01

Numerous small tributaries on the eastern flank of Spearfish Creek originate within a mineralized area with a long history of gold-mining activity. Some streams draining this area are known to have elevated concentrations of arsenic. One such tributary is Annie Creek, where arsenic concentrations regularly approach the Maximum Contaminant Level of 50 mg/L (micrograms per liter) established by the U.S. Environmental Protection Agency. A site on Annie Creek was proposed for inclusion on the National Priorities List by the Environmental Protection Agency in 1991. This report presents information about arsenic loads and concentrations in Spearfish Creek and its tributaries, including Annie Creek. Stream types were classified according to geologic characteris- tics and in-stream arsenic concentrations. The first type includes streams that lack significant arsenic sources and have low in-stream arsenic concentra- tions. The second type has abundant arsenic sources and high in-stream concentrations. The third type has abundant arsenic sources but only moderate in-stream concentrations. The fourth type is a mixture of the first three types. Annual loads of dissolved arsenic were calculated for two reaches of Spearfish Creek to quantify arsenic loads at selected gaging stations during water years 1989-91. Mass-balance calculations also were performed to estimate arsenic concentrations for ungaged inflows to Spearfish Creek. The drainage area of the upstream reach includes significant mineralized areas, whereas the drainage area of the downstream reach generally is without known arsenic sources. The average load of dissolved arsenic transported from the upstream reach of Spearfish Creek, which is representative of a type 4 stream, was 158 kilograms per year, calculated for station 06430900, Spearfish Creek above Spearfish. Gaged headwater tributaries draining unmineralized areas (type 1) contributed only 16 percent of the arsenic load in 63 percent of the discharge. Annie Creek (type 2), which has the highest measured arsenic concentra- tions in the Spearfish Creek drainage, contributed about 15 percent of the arsenic load in about 2 percent of the discharge of the upstream reach. Squaw Creek, which drains another mineralized area, but has only moderate in-stream concentrations (type 3), contributed 4 percent of the arsenic load in 5 percent of the discharge. Ungaged inflows to the reach contributed the remaining 65 percent of the arsenic load in 30 percent of the discharge. The calculated loads from ungaged inflows include all arsenic contributed by surface- and ground-water sources, as well as any additions of arsenic from dissolution of arsenic-bearing solid phases, or from desorption of arsenic from solid surfaces, within the streambed of the upstream reach. Mass-balance calculations indicate that dissolved arsenic concentrations of the ungaged inflows in the upstream reach averaged about 9 mg/L. In-stream arsenic concentrations of ungaged inflows from the unmineralized western flank of Spearfish Creek probably are generally low (type 1). Thus, in-stream arsenic concentrations for ungaged inflows draining the mineralized eastern flank of Spearfish probably average almost twice that level, or about 18 mg/L. Some ungaged, eastern-flank inflows probably are derived from type 3 drainages, with only moderate arsenic concentrations. If so, other ungaged, eastern-flank inflows could have in-stream arsenic concentrations similar to those of Annie Creek. No significant arsenic sources were apparent in the downstream reach of Spearfish Creek. Over the course of the downstream reach, arsenic concentrations decreased somewhat, probably resulting from dilution, as well as from possible chemical adsorption to sediment surfaces or arsenic-phase precipitation. A decrease in arsenic loads resulted from various diversions from the creek and from the potential chemical removal processes. Because of a large margin of error associated with calculation o

5. Historic American Buildings Survey James C. Massey, Photographer 1964 ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

5. Historic American Buildings Survey James C. Massey, Photographer 1964 MAIN (SOUTH) ENTRANCE DETAIL (4x5' b&w film copy neg. from 35mm slide) - Albert F. Madlener House, 4 West Burton Place, Chicago, Cook County, IL

Understanding Country Planning: A Guide for Air Force Component Planners

DTIC Science & Technology

2012-01-01

TRANSPORTATION INTERNATIONAL AFFAIRS LAW AND BUSINESS NATIONAL SECURITY POPULATION AND AGING PUBLIC SAFETY SCIENCE AND TECHNOLOGY TERRORISM AND...example, Jefferson P. Marquis, Richard E. Darilek, Jasen J. Castillo, Cathryn Quantic Thurston, Anny Wong, Cynthia Huger, Andrea Mejia, Jennifer D. P...2006; Jennifer D. P. Moroney, Jefferson P. Marquis, Cathryn Quantic Thurston, and Gregory F. Treverton, A Framework to Assess Programs for Building

Chasing Down Gravitational Wave Sources with the Dark Energy Camera

DOE Office of Scientific and Technical Information (OSTI.GOV)

Annis, Jim; Soares-Santos, Marcelle

On August 17, 2017, scientists using the Dark Energy Camera tracked down the first visible counterpart to a gravitational wave signal ever spotted by astronomers. Using data provided by the LIGO and Virgo collaborations, scientists embarked on a quest for the unknown, and discovered a new wonder of the universe. Includes interviews with Fermilab’s Jim Annis and Brandeis University’s Marcelle Soares-Santos.

Army Reserve Military Intelligence: Time for Change

DTIC Science & Technology

2010-04-20

Miramax Books, 2002. Goleman , Daniel . Emotional Intelligence . New York: Bantam Books, 1997. Goleman , Daniel , Richard Boyatzis, and Annie McKee...or position of the Department of the Army, Department of Defense, or the U.S. Government. ARMY RESERVE MILITARY INTELLIGENCE : TIME FOR CHANGE...Research Project 3. DATES COVERED 00-00-2009 to 00-00-2010 4. TITLE AND SUBTITLE Army Reserve Military Intelligence : Time for Change 5a

Marine and land active-source seismic imaging of mid-Miocene to Holocene-aged faulting near geothermal prospects at Pyramid Lake, Nevada

DOE Office of Scientific and Technical Information (OSTI.GOV)

Eisses, A.; Kell, A.; Kent, G.

Amy Eisses, Annie Kell, Graham Kent, Neal Driscoll, Robert Karlin, Rob Baskin, John Louie, and Satish Pullammanappallil, 2011, Marine and land active-source seismic imaging of mid-Miocene to Holocene-aged faulting near geothermal prospects at Pyramid Lake, Nevada: presented at Geothermal Resources Council Annual Meeting, San Diego, Oct. 23-26.

South Dakota 1995 Kids Count Factbook: Key Indicators of Child Well-Being.

ERIC Educational Resources Information Center

Kids Count--South Dakota, Vermillion.

The South Dakota Kids Count Project began in 1993 with a grant from the Annie E. Casey Foundation. The goal is to improve the collection of national, state, and local data on the well-being of children in a way that increases awareness of their situation and provides the means to address their needs. South Dakota Kids Count tracks children using…

The Power of Plain Talk: Exploring One Program' Influence on the Adolescent Reproductive Health Field

ERIC Educational Resources Information Center

Summerville, Geri; Canova, Karen

2006-01-01

Launched by the Annie E. Casey Foundation in the early 1990s, Plain Talk is a community-based initiative that seeks to reduce the incidence of teen pregnancy and STDs by improving adult/teen communication about sex. A key component of the program is parental involvement--which was once seen by many in the adolescent reproductive health (ARH) field…

Extending Ladders: Findings from the Annie E. Casey Foundation's Jobs Initiative.

ERIC Educational Resources Information Center

Fleischer, Wendy

In 1995, the Anne E. Casey Foundation launched the Jobs Initiative (JI) in six cities to change labor market prospects for low-income young people in order to help them get jobs that could move their families out of poverty. The JI attempts to change the way employers recruit and supervise workers and how work is structured; to prepare workers and…

The Future of Executive-Skills Coaching and Behavioral Science in Programs That Serve Teens and Young Adults: Lessons from the Annie E. Casey Pilot Project

ERIC Educational Resources Information Center

Dechausay, Nadine

2018-01-01

Executive skills are the cognitive abilities that make it possible for people to set goals, regulate impulses, and complete the steps necessary to achieve their objectives. Examples of these skills include time management, emotional control, and organization. Richard Guare and Peggy Dawson have developed a coaching strategy based on executive…

Growing Better Leaders for the Future: A Study in Optimizing Self-Awareness

DTIC Science & Technology

2004-05-03

Creative Leadership Handbook of Leadership Development (Greensboro, NC: Center for Creative Leadership, 1998), 5. 12 Ulmer, 4. 13 Daniel Goleman ...Davies-Black Publishers, 1997. Goleman , Daniel , Richard Boyatzis, and Annie McKee, Primal Leadership, Realizing the Power of Emotional Intelligence...paper recommends two methods for optimizing self-awareness. Those methods are psychological testing and 360-degree feedback. The recommendation for

Berkeley's 'Best-Kept' Secret: National Summit Seeks to Involve Youth in How Communities Are Redeveloped

ERIC Educational Resources Information Center

Burdman, Pamela

2004-01-01

Bringing together youth and adults from 15 Hope VI projects around the country, the Youth Leadership for Change is the third national summit of its kind. HUD used to fund the program, but now support comes from the Annie E. Casey Foundation, the Robert Wood Johnson Foundation and the San Francisco Foundation. At least some participants say the…

Family, Friend, and Neighbor Care: Strengthening a Critical Resource to Help Young Children Succeed. 2006 KIDS COUNT Essay

ERIC Educational Resources Information Center

Annie E. Casey Foundation, 2006

2006-01-01

KIDS COUNT, a project of the Annie E. Casey Foundation, is a national and state-by-state effort to track the status of children in the United States. By providing policymakers and citizens with benchmarks of child well-being, KIDS COUNT seeks to enrich local, state, and national discussions concerning ways to secure better futures for all…

Race for Results: Wisconsin's Need to Reduce Racial Disparities: WisKids Count Policy Brief, Fall 2014

ERIC Educational Resources Information Center

Taylor, Ken

2014-01-01

As the United States continues to become a more diverse nation, much work remains to be done to ensure that all children, regardless of their race or ethnicity, have the opportunity to thrive. A policy report recently published by the Annie E. Casey Foundation found that children of color face immense barriers to success in key categories of…

27. VIEW LOOKING AFT ON STARBOARD SIDE OF MAIN DECK ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

27. VIEW LOOKING AFT ON STARBOARD SIDE OF MAIN DECK WITH TENDER ANNIE RUTH ALONGSIDE. COVER OF FORWARD COMPANIONWAY HAS BEEN PLACED ON MAIN DECK; SUN AWNING A TYPICAL FEATURE IN TROPICAL CLIMATES. CREW MEMBERS UNKNOWN Original 4-3/4'x6-3/4' photograph taken c. 1930? - Pilot Schooner "Alabama", Moored in harbor at Vineyard Haven, Vineyard Haven, Dukes County, MA

Sustaining Neighborhood Change: The Power of Resident Leadership, Social Networks, and Community Mobilization. Making Connections--An Initiative of the Annie E. Casey Foundation

ERIC Educational Resources Information Center

Ahsan, Nilofer

2008-01-01

Poverty and unemployment aren't spread evenly across cities or regions, but rather are concentrated in disinvested urban neighborhoods and rural communities around the country. These communities are home to the nation's most vulnerable children and families. Despite some signs of improvement in economic conditions for families in the United…

Microvolt T-wave alternans and autonomic nervous system parameters can be helpful in the identification of low-arrhythmic risk patients with ischemic left ventricular systolic dysfunction.

PubMed

Daniłowicz-Szymanowicz, Ludmiła; Kaufmann, Damian; Rozwadowska, Katarzyna; Kempa, Maciej; Lewicka, Ewa; Raczak, Grzegorz

2018-01-01

The role of implantable cardioverter-defibrillator (ICD) placement in the primary prevention of sudden cardiac death (SCD) in all consecutive patients with left ventricular ejection fraction (LVEF) ≤ 35% is still a matter of hot debate due to the fact that the population of these patients is highly heterogeneous in terms of the SCD risk. Nevertheless, reduced LVEF is still the only established criterion during qualification of patients for ICD implantation in the primary prevention of SCD, therefore identification of persons with particularly high risk among patients with LVEF ≤35% is currently of lesser importance. More important seems to be the selection of individuals with relatively low risk of SCD in whom ICD implantation can be safely postponed. The aim of the study was to determine whether well-known, non-invasive parameters, such as microvolt T-wave alternans (MTWA), baroreflex sensitivity (BRS) and short-term heart rate variability (HRV), can be helpful in the identification of low-arrhythmic risk patients with ischemic left ventricular systolic dysfunction. In 141 patients with coronary artery disease and LVEF ≤ 35%, MTWA testing, as well as BRS and short-term HRV parameters, were analysed. During 34 ± 13 months of follow-up 37 patients had arrhythmic episode (EVENT): SCD, non-fatal sustained ventricular arrhythmia (ventricular tachycardia [VT] or ventricular fibrillation [VF]), or adequate high-voltage ICD intervention (shock) due to a rapid ventricular arrhythmia ≥200/min. LVEF, non-negative MTWA (MTWA_non-neg), BRS and low frequency power in normalized units (LFnu) turned out to be associated with the incidence of EVENT in univariate Cox analysis. The cut-off values for BRS and LFnu that most accurately distinguished between patients with and without EVENT were 3 ms/mmHg and 23, respectively. The only variable that provided 100% negative predictive value (NPV) for EVENT was negative MTWA result (MTWA_neg), but solely for initial 12 months of the follow-up; the NPVs for other potential predictors of the EVENT were lower. The cut-off values for BRS and LFnu that provide 100% NPV for EVENT during 12 and 24 months were higher: 6.0 ms/mmHg and 73 respectively, but the gain in the NPV occurred at an expense of the number of identified patients. However, the number of identified non-risk patients turned out to be higher when the predictive model included MTWA_neg and the lower cut-off values for ANS parameters: 100% NPV for 12 and 24 months of follow-up was obtained for combination MTWA_neg and BRS ≥ 3 ms/mmHg, for combination MTWA_neg and LFnu ≥ 23 100% NPV was obtained for 12 months. Well-known, non-invasive parameters, such as MTWA, BRS and short-term HRV indices may be helpful in the identification of individuals with a relatively low risk of malignant ventricular arrhythmias among patients with ischemic left ventricular systolic dysfunction; in such persons, implantation of ICD could be safely postponed.

Color infrared film as a negative material

USGS Publications Warehouse

Pease, Robert W.

1970-01-01

Original problems encountered in endeavors to use color infraredfilm as a negative material have been overcome by a simple modification in processing. This makes more feasible the production of infrared color prints for field use and yields an infrared counterpart to Aero-Neg.

LOOKING NORTH AT ENTRY WEST OF BEDROOM WING. Stereopair No. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

LOOKING NORTH AT ENTRY WEST OF BEDROOM WING. Stereopair No. B1983-300-11, Neg. Nos. L6 & R5 - Lake Hotel, .5 mile East of Grand Loop Road & 1.3 miles southwest of Lake Junction, Lake, Teton County, WY

LOOKING SOUTHEAST TOWARDS WEST END OF DINING ROOM. Stereopair No. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

LOOKING SOUTHEAST TOWARDS WEST END OF DINING ROOM. Stereopair No. B1983-300-2, Neg. Nos. L3 & R4 - Lake Hotel, .5 mile East of Grand Loop Road & 1.3 miles southwest of Lake Junction, Lake, Teton County, WY

LOOKING NORTH TOWARDS DINING ROOM AND PORTICO. Stereopair No. B19833004, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

LOOKING NORTH TOWARDS DINING ROOM AND PORTICO. Stereopair No. B1983-300-4, Neg. Nos. L2 & R1 - Lake Hotel, .5 mile East of Grand Loop Road & 1.3 miles southwest of Lake Junction, Lake, Teton County, WY

NEGATIVE-ION MASS SPECTROMETRY OF SULFONYLUREA HERBICIDES

EPA Science Inventory

Sulfonylurea herbicides have been studied using neg-ion desorption chem.-ionization (DCI) mass spectrometry (MS) and DCI-MS/MS techniques. Both {M-H]- and M.- ions were obsd. in the DCI mass spectra. The collisonally activated dissocn. (CAD) spectra were characteristic of the str...

Non-parenchymal liver cells support the growth advantage in the first stages of hepatocarcinogenesis.

PubMed

Drucker, Claudia; Parzefall, Wolfram; Teufelhofer, Olga; Grusch, Michael; Ellinger, Adolf; Schulte-Hermann, Rolf; Grasl-Kraupp, Bettina

2006-01-01

Hepatocellular carcinoma almost always arises in chronically inflamed livers. We developed a culture model to study the role of non-parenchymal cells (NPCs) for inflammation-driven hepatocarcinogenesis. Rats were treated with the carcinogen N-nitrosomorpholine, which induced initiated hepatocytes expressing the marker placental glutathione-S-transferase (GSTp). After 21 days two preparations of hepatocytes were made: (i) conventional ones (Hep-conv) containing NPCs and (ii) hepatocytes purified of NPCs (Hep-pur). Initiated hepatocytes, being positive for GSTp (GSTp-pos) were present in both preparations and were cultured along with normal hepatocytes, being negative for GSTp (GSTp-neg). Under any culture condition DNA synthesis was approximately 4-fold higher in GSTp-pos than in GSTp-neg hepatocytes demonstrating the inherent growth advantage of the first stages of hepatocarcinogenesis. Hepatocytes showed approximately 3-fold lower rates of DNA synthesis in Hep-pur than in Hep-conv, which was elevated above Hep-conv levels by addition of NPC or NPC-supernatant. Pretreatment of NPCs with proinflammatory lipopolysaccharide (LPS) further increased DNA synthesis. Thus, NPCs release soluble growth stimulators. Next we investigated the effect of specific cytokines produced by NPCs. Tumour necrosis factor alpha and interleukin 6 barely altered DNA synthesis, whereas hepatocyte growth factor (HGF), keratinocyte growth factor (KGF) and the heparin-binding epidermal growth factor-like growth factor (HB-EGF) were potent inducers of DNA replication in both, GSTp-neg and GSTp-pos cells. In conclusion, DNA synthesis of hepatocytes is increased by factors released from NPCs, an effect augmented by LPS-stimulation. NPC-derived cytokines, such as KGF, HGF and HB-EGF, stimulate DNA synthesis preferentially in initiated hepatocytes, presumably resulting in tumour promotion. Similar mechanisms may contribute to carcinogenesis in human inflammatory liver diseases.

Meningococco B: controllo di due focolai epidemici mediante vaccinazione

PubMed Central

2014-01-01

Riassunto La problematica di un efficace approccio vaccinale nei confronti del Meningococco B (MenB) è stata superata identificando con la metodica della "reverse vaccinology" alcuni antigeni capaci di indurre una risposta verso la maggior parte dei ceppi di MenB circolanti nel mondo. Il nuovo vaccino MenB a 4 componenti (4CMenB) è stato autorizzato in Europa, Australia e Canada, ed è entrato nei calendari di immunizzazione pediatrica internazionali: Australia, Canada, UK. In Italia, le prime regioni che hanno raccomandato la vaccinazione contro il MenB sono state Basilicata e Puglia. La gestione di epidemie/focolai epidemici richiede la messa in atto di una risposta rapida da parte delle autorità sanitarie nei confronti di una emergenza sanitaria ad elevato impatto, anche emotivo, sulla popolazione, come recentemente dimostrato in due università americane. Alla dichiarazione di focolaio epidemico in atto, in entrambi i contesti si è attivata una procedura per l'uso del vaccino 4CMenB non ancora autorizzato negli USA. È stato così possibile organizzare gli interventi di profilassi attiva nei due campus universitari, adottando il primo impiego su larga scala del nuovo vaccino 4CMenB e conseguendo, in tempi relativamente brevi, elevati tassi di copertura vaccinale. A fronte di circa 14000 studenti immunizzati con almeno una dose, non è stata segnalata alcuna problematica di eventi avversi conseguenti all'immunizzazione; ad oggi non si sono verificati casi nei soggetti che hanno ricevuto il vaccino. Come conseguenza dei due focolai descritti, è oggi in corso la valutazione da parte dell'FDA per l'estensione dell'uso del vaccino 4CMenB negli Stati Uniti negli adolescenti e giovani adulti. PMID:25916017

Predicting weight status stability and change from fifth grade to eighth grade: the significant role of adolescents' social-emotional well-being.

PubMed

Chang, Yiting; Gable, Sara

2013-04-01

The primary objective of this study was to predict weight status stability and change across the transition to adolescence using parent reports of child and household routines and teacher and child self-reports of social-emotional development. Data were from the Early Childhood Longitudinal Study-Kindergarten Cohort (ECLS-K), a nationally representative sample of children who entered kindergarten during 1998-1999 and were followed through eighth grade. At fifth grade, parents reported on child and household routines and the study child and his/her primary classroom teacher reported on the child's social-emotional functioning. At fifth and eighth grade, children were directly weighed and measured at school. Nine mutually-exclusive weight trajectory groups were created to capture stability or change in weight status from fifth to eighth grade: (1) stable obese (ObeSta); (2) obese to overweight (ObePos1); (3) obese to healthy (ObePos2); (4) stable overweight (OverSta); (5) overweight to healthy (OverPos); (6) overweight to obese (OverNeg); (7) stable healthy (HelSta); (8) healthy to overweight (HelNeg1); and (9) healthy to obese (HelNeg2). Except for breakfast consumption at home, school-provided lunches, nighttime sleep duration, household and child routines did not predict stability or change in weight status. Instead, weight status trajectory across the transition to adolescence was significantly predicted by measures of social-emotional functioning at fifth grade. Assessing children's social-emotional well-being in addition to their lifestyle routines during the transition to adolescence is a noteworthy direction for adolescent obesity prevention and intervention. Copyright © 2013 Society for Adolescent Health and Medicine. Published by Elsevier Inc. All rights reserved.

Effect of end-stage renal disease on long-term survival after a first-ever mechanical ventilation: a population-based study.

PubMed

Chen, Chin-Ming; Lai, Chih-Cheng; Cheng, Kuo-Chen; Weng, Shih-Feng; Liu, Wei-Lun; Shen, Hsiu-Nien

2015-10-01

Patients with end-stage renal disease (ESRD(Pos)) usually have multiple comorbidities and are predisposed to acute organ failure and in-hospital mortality. We assessed the effect of ESRD on the poorly understood long-term mortality risk after a first-ever mechanical ventilation (1-MV) for acute respiratory failure. The data source was Taiwan's National Health Insurance (NHI) Research Database. All patients given a 1-MV between 1999 and 2008 from one million randomly selected NHI beneficiaries were identified (n = 38,659). Patients with or without ESRD (ESRD(Neg)) after a 1-MV between 1999 and 2008 were retrospectively compared and followed from the index admission date to death or the end of 2011. ESRD(Pos) patients (n = 1185; mean age: 65.9 years; men: 51.5 %) were individually matched to ESRD(Neg) patients (ratio: 1:8) using a propensity score method. The primary outcome was death after a 1-MV. The effect of ESRD on the risk of death after MV was assessed. A Cox proportional hazard regression model was used to assess how ESRD affected the mortality risk after a 1-MV. The baseline characteristics of the two cohorts were balanced, but the incidence of mortality was higher in ESRD(Pos) patients than in ESRD(Neg) patients (342.30 versus 179.67 per 1000 person-years; P <0.001; covariate-adjusted hazard ratio: 1.43; 95 % confidence interval: 1.31-1.51). For patients who survived until discharge, ESRD was not associated with long-term (>4 years) mortality. ESRD increased the mortality risk after a 1-MV, but long-term survival seemed similar.

Cannabis Use Has Negligible Effects Following Severe Traumatic Injury.

PubMed

AbdelFattah, Kareem R; Edwards, Courtney R; Cripps, Michael W; Minshall, Christian T; Phelan, Herb A; Minei, Joseph P; Eastman, Alexander L

Nearly half of all states have legalized medical marijuana or recreational-use marijuana. As more states move toward legalization, the effects on injured patients must be evaluated. This study sought to determine effects of cannabis positivity at the time of severe injury on hospital outcomes compared with individuals negative for illicit substances and those who were users of other illicit substances. A Level I trauma center performed a retrospective chart review covering subjects over a 2-year period with toxicology performed and an Injury Severity Score (ISS) of more than 16. These individuals were divided into the negative and positive toxicology groups, further divided into the marijuana-only, other drugs-only, and mixed-use groups. Differences in presenting characteristics, hospital length of stay, intensive care unit (ICU) stays, ventilator days, and death were compared. A total of 8,441 subjects presented during the study period; 2,134 (25%) of these had toxicology performed; 843 (40%) had an ISS of more than 16, with 347 having negative tests (NEG); 70 (8.3%) substance users tested positive only for marijuana (MO), 323 (38.3%) for other drugs-only, excluding marijuana (OD), and 103 (12.2%) subjects showed positivity for mixed-use (MU). The ISS was similar for all groups. No differences were identified in Glasgow Coma Scale (GCS), ventilator days, blood administration, or ICU/hospital length of stay when comparing the MO group with the NEG group. Significant differences occurred between the OD group and the NEG/MO/MU groups for GCS, ICU length of stay, and hospital charges. Cannabis users suffering from severe injury demonstrated no detrimental outcomes in this study compared with nondrug users.

National Human Research Ethics: A Preliminary Comparative Case Study of Germany, Great Britain, Romania, and Sweden

PubMed Central

Gallagher, Bernard; Berman, Anne H.; Bieganski, Justyna; Jones, Adele D.; Foca, Liliana; Raikes, Ben; Schiratzki, Johanna; Urban, Mirjam; Ullman, Sara

2016-01-01

Although international research is increasing in volume and importance, there remains a dearth of knowledge on similarities and differences in “national human research ethics” (NHREs), that is, national ethical guidelines (NEGs), Institutional Review Boards (IRBs), and research stakeholder’ ethical attitudes and behaviors (EABs). We begin to address this situation by reporting upon our experiences in conducting a multinational study into the mental health of children who had a parent/carer in prison. The study was conducted in 4 countries: Germany, Great Britain, Romania, and Sweden. Data on NHREs were gathered via a questionnaire survey, two ethics-related seminars, and ongoing contact between members of the research consortium. There was correspondence but even more so divergence between countries in the availability of NEGs and IRBs and in researcher’ EABs. Differences in NHREs have implications particularly in terms of harmonization but also for ethical philosophy and practice and for research integrity. PMID:27746664

Paroxysmal nocturnal haemoglobinuria phenotype cells and leucocyte subset telomere length in childhood acquired aplastic anaemia.

PubMed

Tutelman, Perri R; Aubert, Geraldine; Milner, Ruth A; Dalal, Bakul I; Schultz, Kirk R; Deyell, Rebecca J

2014-03-01

The significance of paroxysmal nocturnal haemoglobinuria (PNH(pos) ) cells and leucocyte subset telomere lengths in paediatric aplastic anaemia (AA) is unknown. Among 22 children receiving immunosuppressive therapy (IST) for AA, 73% (16/22) were PNH(pos) , of whom 94% achieved at least a partial response (PR) to IST; 11/16 (69%) achieved complete response (CR). Only 2/6 (33%) PNH(neg) patients achieved PR. PNH(pos) patients were less likely to fail IST compared to PNH(neg) patients (odds ratio 0·033; 95% confidence interval 0·002-0·468; P = 0·012). Children with AA had short granulocyte (P = 7·8 × 10(-9) ), natural killer cell (P = 6·0 × 10(-4) ), naïve T lymphocyte (P = 0·002) and B lymphocyte (P = 0·005) telomeres compared to age-matched normative data. © 2013 John Wiley & Sons Ltd.

IFLA General Conference, 1989. Division of Libraries Serving the General Public. Section on Children's Libraries; Section of School Libraries; Section of Libraries Serving Disadvantaged Persons. Booklet 30.

ERIC Educational Resources Information Center

International Federation of Library Associations, The Hague (Netherlands).

Fifteen papers from the Division of Libraries Serving the General Public are included in this collection: "When the Children's Library Meets the Museum" (French and English versions; Annie Pissard); "Value of Library Service for Children Literature in France" (Aline Eisenegger); "The Latin American Literature in France" (Aline Eisenegger); "The…

Strategic Military Leaders - Leading Tomorrow

DTIC Science & Technology

2008-02-29

terms. 15 Daniel Goleman , Richard Boyatzis and Annie McKee, Primal Leadership – Learning to Lead with Emotional Intelligence (Boston, MA: Harvard...in war, their role is to dominate and win. Social Intelligence Daniel Goleman defines social intelligence as a combination of two inseparable...October 2006), 3-6. 7 FM6-22, 3-7. 8 Daniel Goleman , Social Intelligence : The New Science of Human Relationships (New York, NY: Bantam Dell, October

National Security Strategy of the United States of America

DTIC Science & Technology

2017-12-18

require fossil fuels, as well as other forms of energy, to power their economies and lift their people out of pover . e United States will...is tyr- anny. For these reasons, our Founders crafted and ratified the Constitution, establishing the repub- lican form of government we enjoy today...it continues to trans- form the future for all nations and all genera- tions. A strong, defensible cyber infrastructure fosters economic growth

Good Jobs And Careers: What Communities Need to do to Train and Move Low-Income, Low-Skilled People into Good Jobs and Careers

ERIC Educational Resources Information Center

Annie E. Casey Foundation, 2005

2005-01-01

In the early 1990s, the Annie E. Casey Foundation (AECF) recognized the importance of addressing economic opportunity as a key component of a larger set of strategies needed to create family economic success for low-income families. Joblessness and lack of family sustaining income have devastating consequences, not only for families, but also for…

Benefits Of Mission Command: Balance Of Philosophy And System

DTIC Science & Technology

2016-05-26

The Benefits of Mission Command: Balance of Philosophy and System A Monograph by MAJ Robert R. Rodock United...Sa. CONTRACT NUMBER The Benefits of Mission Command: Balance of Philosophy and System Sb. GRANT NUMBER Sc. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) Sd... philosophy and system of mission command, when exercised in balance, provides US Anny leaders the agility and adaptability to ’see the elephant’ sooner

Gender, the City and the Politics of Schooling: Towards a Collective Biography of Women "Doing Good" as Public Moralists in Victorian London

ERIC Educational Resources Information Center

Martin, Jane

2005-01-01

This article tells the stories of four middle class, white, English women whose participation in educational policy making is little known: Annie Leigh Browne (1851-1936), Margaret MacDonald (1870-1911), Hilda Miall-Smith (born 1861) and Honnor Morten (1861-1913). In doing so, it provides a perspective on the circumstances that enabled or…

Army Blast Claims Evaluation Procedures

DTIC Science & Technology

1994-03-01

ARMY RESEARCH LABORATORY ARL-MR-131 Army Blast Claims Evaluation Procedures William P. Wright APPROVED FOR PUBUC RELEASE; DISTRIBtmON IS...NUMBERS Anny Blast Claims Evaluation Procedures 4G061-304-U2 6. AUTHOR(S) William P. Wrisht 1. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) 8...of the technical review process which bas been instituted to develop an opinion as to Army responsibility. 14. SUBJECT TERMS blast effects. muzzle

Celebrating John Glenn’s Legacy

NASA Image and Video Library

2012-03-02

Wife of former astronaut and Senator John Glenn, Annie Glenn, listens intently to Cleveland State University Master of Music Major James Binion Jr. as he sings a musical tribute during an event celebrating John Glenn's legacy and 50 years of americans in orbit held at the university's Wolstein Center on Friday, March 3, 2012 in Cleveland, Ohio. Glenn became the first American to orbit Earth in 1962. Photo Credit: (NASA/Bill Ingalls)

Meeting the Challenges of Educational Change for Indian People. The 11th Annual Report to the Congress of the United States. Fiscal Year 1984.

ERIC Educational Resources Information Center

National Advisory Council on Indian Education, Washington, DC.

The 11th annual report to Congress from the National Advisory Council on Indian Education (NACIE) is dedicated to Navajo educational advocate Annie Wauneka and details activities during fiscal year 1984. Part I contains an introduction, an overview of the Council, legislative history, and lists of council members and committees. Part II details…

KIDS COUNT Data Book, 2014: State Trends in Child Well-Being. 25th Edition

ERIC Educational Resources Information Center

Annie E. Casey Foundation, 2014

2014-01-01

Each year since 1990, the Annie E. Casey Foundation has published the KIDS COUNT Data Book to track the well-being of children nationally and in every state. When the first Data Book was launched 25 years ago, the hope was that it would raise public awareness and build public commitment to invest in solutions to ensure that each and every child…

Review of Criminal Investigations of Alleged Detainee Abuse

DTIC Science & Technology

2006-08-25

reporting requirements published in Anny Regulation (AR) 190-40, "Serious Incident Report," November 30, 1993.13 A serious incident is "[a]ny actual...investigated by commanders under the authority of this regulation . In its purpose statement, the regulation states that the policy is limited to...provisions of this regulation , when made applicable, and the provisions of the specific directive authorizing the 15 AR 190-40, Serious Incident Report

Sharing Stories: Community/Science Collaboration and Learning As We Go

NASA Astrophysics Data System (ADS)

Marino, E.; Weyiouanna, A.

2017-12-01

*Annie Weyiouanna is the first author of this presentation - because of AGU funding and submission procedures, Elizabeth Marino is incorrectly identified in the program and online as first author. In Shishmaref, Alaska, climate change is rapidly affecting many aspects of life including subsistence hunting, and seasonal gathering. Also critical is that climate change is creating flooding & erosion on the island that could threaten the safety of the community in the future. Because of this, community members, along with social and natural scientists have been vocal about the fact that something needs to be done to ensure the safety of the community - whether that means protecting the island sufficiently in place, or relocating community members off of the island, or both. In this presentation, Annie Weyiouanna will discuss the seasonal patterns on the island and the effect that climate change has had on her community; Elizabeth Marino will follow up with her perspective as an outsider who has studied the historical construction of risk in Shishmaref. Finally the two will discuss, going forward, how scientists should work to open up avenues for community members to voice their own stories and experiences; and how understanding the links between policy possibilites and local experiences might be a good step going forward to instigate positive change and safety for at risk communities.

75 FR 5346 - Comment Request for Information Collection for Workforce Investment Act National Emergency Grant...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-02-02

... Emergency Grants (NEGs) which are discretionary grants intended to temporarily expand the service capacity... DEPARTMENT OF LABOR Comment Request for Information Collection for Workforce Investment Act National Emergency Grant Program: Application and Reporting Procedures (OMB Control No. 1205-0439...

North Dakota | Solar Research | NREL

Science.gov Websites

customer's load are owned by net-metered customers, while RECs associated with NEG are owned by the utility . Meter aggregation: Not addressed Interconnection There are currently no specific interconnection unclear. Community Solar There are currently no statewide community solar policies in North Dakota. State

Effects of processing variables on the sensitometric properties of Eastman Kodak film type 2445

NASA Technical Reports Server (NTRS)

Weinstein, M. S.

1972-01-01

The sensitometric characteristics of Kodak color negative film Type 2445 with processing in a 1411 versamat with Kodak aero-neg. color process chemicals are described. These characteristics were determined for various combinations of machine speed, prehardener temperature, and first developer temperature.

Investigation of Shallow Undex in Littoral Ocean Domain

DTIC Science & Technology

2014-06-01

pPos Units=Pa #Units: psi, ksi, Pa, MPa, bar or scaling factor # Var=pNeg Units=Pa #Units: psi, ksi, Pa, MPa...net) # i = Impulse intensity (time in sec) # ppos = Element pressure on "positive" side (normal points toward "eye") of coupling interface

78 FR 67351 - Combined Notice of Filings

Federal Register 2010, 2011, 2012, 2013, 2014

2013-11-12

... Company submits tariff filing per 154.204: Continental Resources Agreement to be effective 11/1/2013... Company LLC submits tariff filing per 154.204: Amendment to Neg Rate Agmt (BP 37-12) to be effective 10/31...: Docket Numbers: RP14-81-000. Applicants: Questar Pipeline Company. Description: Questar Pipeline Company...

Spatial variability of sugarcane yields in relation to soil salinity in Louisiana

USDA-ARS?s Scientific Manuscript database

High soil salinity levels have been documented to negatively impact sugarcane yields. Tests were conducted in commercial sugarcane fields in South Louisiana in 2009-2010 to determine if elevated soil salinity levels resulting from salt water intrusion from several recent hurricanes was having a neg...

The Northeast Greenland Shelf - Evidence of the existence of a pronounced salt-province

NASA Astrophysics Data System (ADS)

Schmitz, T.; Jokat, W.

2003-04-01

The Northeast Greenland shelf (NEGS) is the part of the continental margin of east Greenland located between the Jan Mayen Fracture Zone at about 72°N in the south and the Spitzbergen Fracture Zone at 81°N in the north. The eastern boundary, at the shelf edge, is the approximate position of the boundary between continental and oceanic crust and the western boundary is the coastline of Greenland. The shelf has a N-S orientation, is about 1000 km long, and between 125 km (southern part) and 380 km (at 78°N) wide. Based on present data the NEGS can be subdivided into a southern part influenced by Tertiary tectonism and volcanism (approx. 72°N to 75°N) and a northern, nonvolcanic, part (approx. 75°N to 81°N). Today the sedimentary history, stratigraphy, structure and origin of the basement below the sedimentary shelf south of 74°N are reasonable known, but only sparse information exists about the northern part of the shelf. Until 1990 there weren't any seismic lines north of 74°N, and all interpretations of stratigraphy and basin structures of the northern part of the NEGS were based on aeromagnetic data. During the last decade, the first seismic lines were shot over the northern part of the shelf to give more detailed information about sediment thickness, stratigraphy, and the structure of the sedimentary shelf. The area under investigation lies on the nonvolcanic northern part of the shelf between 78°30'N and 81°N. The sea floor topography indicates some submarine banks with water depth as shallow as 30 m, which are separated by valleys up to 500 m deep. These valleys were formed through erosion processes caused by cyclic movements of big grounded glacier tongues during the last ice-ages with a maximum expansion during the Wisconsin-Weichselian glaciation. During two scientific expeditions with the German research icebreaker Polarstern in 1997 and 1999, more than 1100 km of multichannel seismic data were collected. The cruise tracks during seismic measurement were diverted by heavy ice. The seismic equipment consisted of a 64-channel streamer (1600m long), 14 sonobuoys for data acquisition and an airgun cluster with a total volume of 24l (8 VLF airguns) as seismic source. Analysis of the results shows an upper sediment layer of 600 to 1000 m thickness with apparent velocities between 2.5 and 3.8 km/s. A second layer of up to 4600 m thickness has apparent velocities between 4.2 and 5.2 km/s. The sediment thickness increases towards the continental slope. The NEGS appears to be a glacial shaped shelf with intense eroded and compacted sediments near the sea floor which are caused by the cyclic advancement and retreat of the Greenland Ice Sheet. These compacted sediments generate the unusually high apparent velocities right under the sea floor. The seismic records from the shelf are heavily superimposed by sea floor multiples which are due to the overcompacted and very hard sea floor. The seismic reflection lines indicate sedimentary structures which we interpret as a province of salt domes, salt ridges and salt walls which reaches more than 90 km further north than mentioned by Escher and Pulvertaft in 1995. Negative anomalies in the shipborne gravity data as well as satellite measurements from over the NEGS support this interpretation.

US Army Evaluations: A Study of Inaccurate and Inflated Reporting

DTIC Science & Technology

2012-04-26

decisions such as promotions directly impacting the careers of tens of thousands of the Anny’s leaders, both officer and NCO, has few equals in the...accurate, and equitable perfonnance ratings throughout the Army.13 Many ofthe revisions were caused by the inability of selection boards to discern a...should be : assigned a numerical percentage; superior equals top ten percent, excellence equals top twenty- five percent, success equals top fifty

KIDS COUNT Data Book, 2011: State Profiles of Child Well-Being. America's Children, America's Challenge: Promoting Opportunity for the Next Generation

ERIC Educational Resources Information Center

Annie E. Casey Foundation, 2011

2011-01-01

For the past 21 years, the KIDS COUNT project of the Annie E. Casey Foundation has tracked the well-being of children at the national, state, and local levels. Over the years, the foundation's work has documented both great progress in child well-being and periodic setbacks. The 10 key indicators tracked in the KIDS COUNT Data Book over the past…

Anny Cazenave Receives 2012 William Bowie Medal: Response

NASA Astrophysics Data System (ADS)

Cazenave, Anny

2013-01-01

It is a great pleasure, a privilege, and an immense honor to receive the AGU Bowie Medal. When I look at the list of previous recipients, with so many prestigious well-known names, I can hardly realize that I have also been awarded this prestigious medal. I feel very humble, following on from these highly distinguished scientists. Thanks very much, Dick, for your kind citation and thanks to the colleagues who have supported my nomination.

Marine and land active-source seismic investigation of geothermal potential, tectonic structure, and earthquake hazards in Pyramid Lake, Nevada

DOE Office of Scientific and Technical Information (OSTI.GOV)

Eisses, A.; Kell, A.; Kent, G.

Amy Eisses, Annie M. Kell, Graham Kent, Neal W. Driscoll, Robert E. Karlin, Robert L. Baskin, John N. Louie, Kenneth D. Smith, Sathish Pullammanappallil, 2011, Marine and land active-source seismic investigation of geothermal potential, tectonic structure, and earthquake hazards in Pyramid Lake, Nevada: presented at American Geophysical Union Fall Meeting, San Francisco, Dec. 5-9, abstract NS14A-08.

Space and U.S. Security. A Net Assessment

DTIC Science & Technology

2009-01-01

spacecraft mission. This included not only a spacewalk by a Chinese astronaut, but also a companion satellite. China demonstrated the ability to release...telecommunications, the Italian Armed Forces use the SICRAL ( Sistema Italiana de Communicazione Riservente Allarmi) system. Thus far, one satellite

Validation of a Competitive Enzyme-Linked Immunosorbent Assay for Detection of Babesia bigemina Antibodies in Cattle

USDA-ARS?s Scientific Manuscript database

A competitive ELISA (cELISA) based on a broadly conserved, species-specific, B-cell epitope within the C-terminus of Babesia bigemina rhoptry-associated protein-1a was validated for international use. Receiver Operating Characteristic (ROC) analysis revealed 16% inhibition as the threshold for a neg...

ESTIMATION OF CRITICAL LOADS OF ACIDITY FOR LAKESIN NORTHEASTERN UNITED STATES AND EASTERN CANADA

EPA Science Inventory

The New England Governors and Eastern Canadian Premiers (NEG/ECP) adopted the Acid Rain Action Plan in June 1998, and issued a series of action items to support its work toward a reduction of sulfur dioxide (SO2) and nitrogen oxide (NOx ) emissions in northeastern North Americ...

Analytical Tripping Loads for Stiffened Plates

DTIC Science & Technology

1993-12-01

extensional strains at the midsurface of the plate are negli- gible. 2 (vii) The incremental buckling displacements may be approximated by the...extensional strains at the midsurface of the plate are given by 3 el = ul + ! e12 = +VI + WIW2), e22 = V2 + 2 The strains in the beam are denoted by

75 FR 53672 - Takes of Marine Mammals Incidental to Specified Activities; Taking Marine Mammals Incidental to...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-01

... published in December 2006. For a detailed description and calculation of the cetacean abundance data and... the proposed Northeast Gateway Port and Pipeline Lateral. A notice of availability was published by... Harassment Authorization (IHA) to the Northeast Gateway Energy Bridge TM LP (Northeast Gateway or NEG) and...

An examination of the psychometric properties of the Rosenberg Self-Esteem Scale (RSES) in Chinese acute coronary syndrome (ACS) patients.

PubMed

Martin, Colin R; Thompson, David R; Chan, Dominic S

2006-11-01

The psychometric properties of the Rosenberg Self-Esteem Scale (RSES) as a clinical research instrument for acute coronary syndrome (ACS) patients were investigated in a translated Chinese version of the instrument. A confirmatory factor analysis was conducted on the RSES to establish its psychometric properties in 128 ACS patients over two observation points (within 1 week and 6 months post-admission for ACS). Internal and test-retest reliability of the RSES-TOT (all-items) and RSES-POS sub-scale (positively valenced items) were found to be acceptable. The RSES-NEG sub-scale (negatively valenced items) lacked acceptable internal reliability. The underlying factor structure of the RSES comprised two distinct but related factors, though there was inconsistency in best model fit indices at the 1-week observation point. The use of the RSES as two sub-scales (RSES-POS and RSES-NEG) may be clinically useful in evaluating the influence of this important psychological construct on the health outcomes of patients with ACS. Directions for future research are indicated.

The nucleosomal acidic patch relieves auto-inhibition by the ISWI remodeler SNF2h

PubMed Central

Gamarra, Nathan; Johnson, Stephanie L; Trnka, Michael J; Burlingame, Alma L

2018-01-01

ISWI family chromatin remodeling motors use sophisticated autoinhibition mechanisms to control nucleosome sliding. Yet how the different autoinhibitory domains are regulated is not well understood. Here we show that an acidic patch formed by histones H2A and H2B of the nucleosome relieves the autoinhibition imposed by the AutoN and the NegC regions of the human ISWI remodeler SNF2h. Further, by single molecule FRET we show that the acidic patch helps control the distance travelled per translocation event. We propose a model in which the acidic patch activates SNF2h by providing a landing pad for the NegC and AutoN auto-inhibitory domains. Interestingly, the INO80 complex is also strongly dependent on the acidic patch for nucleosome sliding, indicating that this substrate feature can regulate remodeling enzymes with substantially different mechanisms. We therefore hypothesize that regulating access to the acidic patch of the nucleosome plays a key role in coordinating the activities of different remodelers in the cell. PMID:29664398

U.S. Leading from Behind in Libya? Thoughts for NATO before the Chicago Summit

DTIC Science & Technology

2012-04-25

degli anni 1990, la linea di base della tecnologia e business divenne inglese. Paesi come la Francia abbandonarono la loro resistenza e ha ceduto...bene." 20 I social media su internet e il progresso della tecnologia smart phone ha dato vita a un linguaggio comune delle informazioni 24/7. Un...appello rivolto su NATO Review del Vice Segretario generale aggiunto per gli armamenti, e coordinatore della tecnologia per l‘antiterrorismo

The Magic Background of Pearl Harbor. Volume 4. Appendix

DTIC Science & Technology

1977-01-01

Febbaio 1939, il Manchiukuo col protocollo del 24 Febbraio 1939 e la Spagna col protocollo del 27 Marzo 1939, viene prorogato per cinque anni a... de - layed. . The new cabinet differs in no way from the former one in its sincere desire to adjust Japanese- United States relations on a fair basis...34DoDcomment:Nameithh ld. Trans. 10-21-41 A-3 No.7 FROM:TO: Washington (Nomura) Tokyo October 24 , 1941 #989.eour#698

Don’t Ask, Don’t Tell: Law or DoD Policy Deciphering the Differences

DTIC Science & Technology

2009-12-11

the Gay Ban Undermines the Military and Weakens America. New York: St Martin’s Press, 2009. Goleman , Daniel , Richard E. Boyatzis, and Annie McKee...Primal Leadership: Learning to Lead with Emotional Intelligence . Harvard: Harvard Business Press, 2004. Hall, Kermit L. The Oxford Companion to...differences in the language and the policies in order to speak intelligently about the issues and propose solutions that are logical and in consonance with

Patents - An Information Resource.

DTIC Science & Technology

1980-09-01

11 vs sans dire que ces aspects de description at de divulqation interviennent en plus des trois critbrea classiques de nouveautd, de hauteur...la divulgation doit, nous 1’ avons vu, aux termes de Ia loi , permettre b l’homme de l’art de reconstituer 1’invention. Or on sait bien que lea d...je vous en donnerai confirmation au mayen de r~sultats dletudea conduites au cours de ceo dernibres annies dans diff~rents pays et secteurs

Women as Members of Groups. Second Grade Social Studies: Elizabeth Cady Stanton, Elizabeth Blackwell, M.D., Annie Wauneka, Rosa Parks, Dolores Huerta, [and] Shirley Cachola, M.D.

ERIC Educational Resources Information Center

National Women's History Project, Santa Rosa, CA.

Part of the National Women's History Project funded to promote the study of women in history, this unit will help second grade students learn about women's contributions to U.S. society. Equity cannot be achieved until equality is expected and until the contributions of all women are understood and accepted as a simple matter of fact. The unit…

Beyond Hate: Countering Violent Extremism from the White Power Movement

DTIC Science & Technology

2013-09-01

Wilson, J. C. Lester, and Walter Lynwood Fleming, Ku Klux Klan Its Origin, Growth and Disbandment, n.d., Kindle edition, loc 109 of 2871. 40...Chalmers, Hooded Americanism, 16–17. 41 Wilson, Lester, and Fleming, Ku Klux Klan Its Origin, Growth and Disbandment, Kindle edition, loc 153 of 2871...Lester, and Fleming, Ku Klux Klan Its Origin, Growth and Disbandment, loc 152 of 2871; Annie Cooper Burton, The Ku Klux Klan (Los Angeles, California

Supporting Meteorological Field Experiment Missions and Postmission Analysis with Satellite Digital Data and Products

DTIC Science & Technology

2011-08-01

of geostationary (GEO) and low-earth-orbiting (LEO) sensors were employed to help guide daily mission planning , forecasts, and outlooks, and also...to enhance postmission analysis studies. This paper chronicles the T-PARC/TCS-08 project’s satellite-observing tools, imagery, and de - rived...how satellite-based remote sensing can be optimized to provide dedicated field campaign support. ReAl-Time miSSiON PlANNiNg , NOw- CASTiNg, AND

Influence of mining-related activities on concentrations of metals in water and sediment from streams of the Black Hills, South Dakota.

PubMed

May, T W; Wiedmeyer, R H; Gober, J; Larson, S

2001-01-01

Water and sediment samples were collected from streams in Spearfish Creek, Whitewood Creek, and Bear Butte Creek watersheds in the Black Hills, SD, an area impacted by gold mining operations. Arsenic concentrations that exceeded the U.S. Environmental Protection Agency's Maximum Concentration Limit of 50 microg/L for drinking water were found in water from Annie Creek, a tributary of Spearfish Creek, and from Whitewood Creek. Gold Run, a tributary of Whitewood Creek, and Annie Creek contained Se concentrations in water that exceeded the EPA Ecotox threshold of 5 microg/L and were classified as a high hazard for Se accumulation from water into the planktonic food chain and for resultant toxicity to fish and aquatic birds. Concentrations of As, Cd, Cu, Hg, Ni, Pb, and Zn in sediment exceeded EPA Ecotox thresholds in one or more of the watersheds suggesting potential adverse ecological effects. Sediment from Rubicon Creek, a tributary of Spearfish Creek, contained Se concentrations high enough (4.0 microg/g) to be a moderate hazard for accumulation from sediments into the benthic food chain, with resultant dietary toxicity to fish and aquatic birds. These results are discussed in light of historical mining activities and recent clean-up and reclamation efforts. Based on the results and comparisons to Ecotox tresholds, further studies of ecological effects are warranted.

Influence of mining-related activities on concentrations of metals in water and sediment from streams of the Black Hills, South Dakota

USGS Publications Warehouse

May, T.W.; Wiedmeyer, Ray H.; Gober, J.; Larson, S.

2001-01-01

Water and sediment samples were collected from streams in Spearfish Creek, Whitewood Creek, and Bear Butte Creek watersheds in the Black Hills, SD, an area impacted by gold mining operations. Arsenic concentrations that exceeded the U.S. Environmental Protection Agency's Maximum Concentration Limit of 50 μg/L for drinking water were found in water from Annie Creek, a tributary of Spearfish Creek, and from Whitewood Creek. Gold Run, a tributary of Whitewood Creek, and Annie Creek contained Se concentrations in water that exceeded the EPA Ecotox threshold of 5 μg/L and were classified as a high hazard for Se accumulation from water into the planktonic food chain and for resultant toxicity to fish and aquatic birds. Concentrations of As, Cd, Cu, Hg, Ni, Pb, and Zn in sediment exceeded EPA Ecotox thresholds in one or more of the watersheds suggesting potential adverse ecological effects. Sediment from Rubicon Creek, a tributary of Spearfish Creek, contained Se concentrations high enough (4.0 μg/g) to be a moderate hazard for accumulation from sediments into the benthic food chain, with resultant dietary toxicity to fish and aquatic birds. These results are discussed in light of historical mining activities and recent clean-up and reclamation efforts. Based on the results and comparisons to Ecotox tresholds, further studies of ecological effects are warranted.

Accumulation of carboxymethyl-lysine (CML) in human cortical bone.

PubMed

Thomas, Corinne J; Cleland, Timothy P; Sroga, Grazyna E; Vashishth, Deepak

2018-05-01

Advanced glycation end-products (AGEs) are a category of post translational modification associated with the degradation of the structural properties of multiple different types of tissues. Typically, AGEs are the result of a series of post-translational modification reactions between sugars and proteins through a process known as non-enzymatic glycation (NEG). Increases in the rate of NEG of bone tissue are associated with type 2 diabetes and skeletal fragility. Current methods of assessing NEG and its impact on bone fracture risk involve measurement of pentosidine or total fluorescent AGEs (fAGEs). However, pentosidine represents only a small fraction of possible fAGEs present in bone, and neither pentosidine nor total fAGE measurement accounts for non-fluorescent AGEs, which are known to form in significant amounts in skin and other collagenous tissues. Carboxymethyl-lysine (CML) is a non-fluorescent AGE that is often measured and has been shown to accumulate in tissues such as skin, heart, arteries, and intervertebral disks, but is currently not assessed in bone. Here we show the localization of CML to collagen I using mass spectrometry for the first time in human bone. We then present a new method using demineralization followed by heating and trypsin digestion to measure CML content in human bone and demonstrate that CML in bone is 40-100 times greater than pentosidine (the current most commonly used marker of AGEs in bone). We then establish the viability of CML as a measurable AGE in bone by showing that levels of CML, obtained from bone using this technique, increase with age (p<0.05) and are correlated with previously reported measures of bone toughness. Thus, CML is a viable non-fluorescent AGE target to assess AGE accumulation and fragility in bone. The method developed here to extract and measure CML from human bone could facilitate the development of a new diagnostic assay to evaluate fracture risk and potentially lead to new therapeutic approaches to address bone fragility. Copyright © 2018 Elsevier Inc. All rights reserved.

Integrated DNA methylation and copy-number profiling identify three clinically and biologically relevant groups of anaplastic glioma.

PubMed

Wiestler, Benedikt; Capper, David; Sill, Martin; Jones, David T W; Hovestadt, Volker; Sturm, Dominik; Koelsche, Christian; Bertoni, Anna; Schweizer, Leonille; Korshunov, Andrey; Weiß, Elisa K; Schliesser, Maximilian G; Radbruch, Alexander; Herold-Mende, Christel; Roth, Patrick; Unterberg, Andreas; Hartmann, Christian; Pietsch, Torsten; Reifenberger, Guido; Lichter, Peter; Radlwimmer, Bernhard; Platten, Michael; Pfister, Stefan M; von Deimling, Andreas; Weller, Michael; Wick, Wolfgang

2014-10-01

The outcome of patients with anaplastic gliomas varies considerably. Whether a molecular classification of anaplastic gliomas based on large-scale genomic or epigenomic analyses is superior to histopathology for reflecting distinct biological groups, predicting outcomes and guiding therapy decisions has yet to be determined. Epigenome-wide DNA methylation analysis, using a platform which also allows the detection of copy-number aberrations, was performed in a cohort of 228 patients with anaplastic gliomas (astrocytomas, oligoastrocytomas, and oligodendrogliomas), including 115 patients of the NOA-04 trial. We further compared these tumors with a group of 55 glioblastomas. Unsupervised clustering of DNA methylation patterns revealed two main groups correlated with IDH status: CpG island methylator phenotype (CIMP) positive (77.5 %) or negative (22.5 %). CIMP(pos) (IDH mutant) tumors showed a further separation based on copy-number status of chromosome arms 1p and 19q. CIMP(neg) (IDH wild type) tumors showed hallmark copy-number alterations of glioblastomas, and clustered together with CIMP(neg) glioblastomas without forming separate groups based on WHO grade. Notably, there was no molecular evidence for a distinct biological entity representing anaplastic oligoastrocytoma. Tumor classification based on CIMP and 1p/19q status was significantly associated with survival, allowing a better prediction of outcome than the current histopathological classification: patients with CIMP(pos) tumors with 1p/19q codeletion (CIMP-codel) had the best prognosis, followed by patients with CIMP(pos) tumors but intact 1p/19q status (CIMP-non-codel). Patients with CIMP(neg) anaplastic gliomas (GBM-like) had the worst prognosis. Collectively, our data suggest that anaplastic gliomas can be grouped by IDH and 1p/19q status into three molecular groups that show clear links to underlying biology and a significant association with clinical outcome in a prospective trial cohort.

Human intrahepatic ILC2 are IL-13positive amphiregulinpositive and their frequency correlates with model of end stage liver disease score.

PubMed

Jeffery, Hannah C; McDowell, Patrick; Lutz, Philipp; Wawman, Rebecca E; Roberts, Sheree; Bagnall, Chris; Birtwistle, Jane; Adams, David H; Oo, Ye Htun

2017-01-01

Innate lymphoid cells (ILC) have been implicated in the initiation of inflammation and fibrosis in mice. However, ILC have not been characterized in inflamed human liver tissue. Human intrahepatic lymphocytes were isolated by mechanical digestion and phenotyped by flow cytometry. Conditioned medium from cultures of primary human biliary epithelial cells, stellate cells, fibroblasts and inflamed human liver tissue was used to model the effects of the inflammatory liver environment of ILC phenotype and function. All three ILC subsets were present in the human liver, with the ILC1 (CRTH2negCD117neg) subset constituting around 70% of intrahepatic ILCs. Both NCRpos (NKp44+) and NCRneg ILC3 (CRTH2negCD117pos) subsets were also detected. ILC2 (CRTH2pos) frequency correlated with disease severity measured by model of end stage liver disease (MELD) scoring leading us to study this subset in more detail. ILC2 displayed a tissue resident CD69+ CD161++ phenotype and expressed chemokine receptor CCR6 allowing them to respond to CCL20 secreted by cholangiocytes and stellate cells. ILC2 expressed integrins VLA-5 and VLA-6 and the IL-2 and IL-7 cytokine receptors CD25 and CD127 although IL-2 and IL-7 were barely detectable in inflamed liver tissue. Although biliary epithelial cells secrete IL-33, intrahepatic ILC2 had low expression of the ST2 receptor. Intrahepatic ILC2 secreted the immunoregulatory and repair cytokines IL-13 and amphiregulin. Intrahepatic ILC2 express receptors allowing them to be recruited to bile ducts in inflamed portal tracts. Their frequencies increased with worsening liver function. Their secretion of IL-13 and amphiregulin suggests they may be recruited to promote resolution and repair and thereby they may contribute to ongoing fibrogenesis in liver disease.

Deeply reinverted T wave at 14 days after the onset of first anterior acute myocardial infarction predicts improved left ventricular function at 6 months.

PubMed

Yokoyama, Hiroaki; Tomita, Hirofumi; Nishizaki, Fumie; Hanada, Kenji; Shibutani, Shuji; Yamada, Masahiro; Abe, Naoki; Higuma, Takumi; Osanai, Tomohiro; Okumura, Ken

2015-03-01

Changes in electrocardiogram (ECG), especially in the ST segment and T wave, have been recognized as a noninvasive diagnostic tool for coronary flow or myocardial injury. A deeply inverted T wave at 14 days after successful percutaneous coronary intervention (PCI) in patients with ST-segment elevation acute myocardial infarction (STEMI) predicts improved left ventricular (LV) function at 6 months. We enrolled 112 consecutive patients (88 men, 63 ± 11 years) with first anterior STEMI who underwent successful PCI. A 12-lead ECG was recorded everyday from admission through 14 days. After PCI, the first T-wave inversion was observed within 2 days, and the second occurred at 14 days. We measured the maximum depth of the reinverted T wave (Neg-T) and divided the patients into 2 groups based on the median value of Neg-T: the deep group (≥0.6 mV, n = 62) and the nondeep group (<0.6 mV, n = 50). LV ejection fraction (LVEF) at 14 days did not differ between the 2 groups, but it was greater in the deep than in the nondeep group at 6 months (50.0% ± 8.8% vs 42.5% ± 9.8 %, P < 0.0001). The maximum creatinine phosphokinase-myocardial band (CPK-MB) value was significantly lower in the deep than in the nondeep group. Reappearance of the R wave in precordial leads at 6 months was more frequently observed in the deep than in the nondeep group (68% vs 46%, P = 0.02). Multivariate regression analysis showed that the Neg-T and max CPK-MB were independent contributors to LVEF at 6 months. A deeply reinverted T wave at 14 days after onset of first anterior STEMI can be a useful predictive marker for improved LV function at 6 months. © 2015 Wiley Periodicals, Inc.

Vitamin E and selenium levels are within normal range in pigs diagnosed with mulberry heart disease and evidence for viral involvement in the syndrome is lacking.

PubMed

Shen, H; Thomas, P R; Ensley, S M; Kim, W-I; Loynachan, A T; Halbur, P G; Opriessnig, T

2011-12-01

Mulberry heart disease (MHD) in pigs is characterized by lesions of acute haemorrhagic myocarditis and myocardial necrosis. The objectives of this study were to determine the levels of vitamin E and selenium and 13 other trace minerals in heart and liver tissues and to determine the prevalence of certain viral infections in heart tissues from MHD-affected and MHD-unaffected pigs and the vitamin E and selenium concentration in feed samples from selected farms with MHD. Based on the pathological examination, 114 pigs were separated into MHD lesion-negative (L-NEG) (n = 57) and MHD lesion-positive (L-POS) (n = 57) groups. Seventy-three samples (40 L-NEG and 33 L-POS) were subjected to chemical analysis, and 66 (32 L-NEG and 34 L-POS) were subjected to PCR detection for viral pathogens. Lower (P < 0.05) levels of myocardial copper, lower (P < 0.05) levels of hepatic magnesium and higher (P < 0.05) levels of myocardial and hepatic sodium were detected in the L-POS cases. Although lower (P < 0.05) levels of hepatic selenium were detected in L-POS group, all were within the normal range. Analysis of feed samples (n = 22) revealed that selenium levels in all the samples were above the legal limit (0.3 ppm) for pigs. Vitamin E levels in all feed samples were above 20 IU/kg. Among the 66 pigs subjected to PCR detection, there were 19, 4, 13, 8, 2 and 1 animals positive for porcine circovirus type 2, porcine reproductive and respiratory syndrome virus, pan-herpes virus, porcine enterovirus, pan-pestivirus and porcine parvovirus, respectively. Clear evidence of viral association with L-POS was lacking. © 2011 Blackwell Verlag GmbH.

Effects of probiotics and application methods on performance and response of broiler chickens to an Eimeria challenge.

PubMed

Ritzi, Miranda M; Abdelrahman, Wael; Mohnl, Michaela; Dalloul, Rami A

2014-11-01

Coccidiosis is an inherent risk in the commercial broiler industry and inflicts devastating economic losses to poultry operations. Probiotics may provide a potential alternative to the prophylactic use of anticoccidials in commercial production. This study evaluated the effects of probiotic applications (feed and water) on bird performance and resistance to a mixed Eimeria infection in commercial broilers. On day of hatch, 1,008 commercial male broilers (Cobb 500) were assigned to 1 of 6 treatments (8 replicate floor pens; 21 birds/pen), including noninfected negative control (NEG), Eimeria-infected positive control (POS), anticoccidial control (0.01% salinomycin, SAL), intermittent high-dose water-applied probiotic (WPI), continuous low-dose water-applied probiotic (WPC), and feed-supplemented probiotic (FSP). On d 15, all birds except those in NEG were challenged with a mixed inoculum of Eimeria acervulina, Eimeria maxima, and Eimeria tenella. Measurements were taken on d 7, 15, 21, 28, 35, and 42. Fecal samples were collected from d 20 to 24 for oocyst counts, and lesion scores were evaluated on d 21. Data were analyzed using the Fit Model platform in JMP Pro 10.0 (SAS Institute Inc.). Differences in experimental treatments were tested using Tukey's honestly significant difference following ANOVA with significance reported at P ≤ 0.05. Overall, NEG birds outperformed all other groups. For performance, the probiotic groups were comparable with the SAL-treated birds, except during the 6 d immediately following the Eimeria species challenge, where the SAL birds exhibited better performance. The WPC birds had lower duodenal and jejunal lesion scores, indicating a healthier intestine and enhanced resistance to Eimeria species compared with POS. Birds in the WPI treatment shed fewer oocysts in the feces, although this was not a trend for all of the probiotic treatment groups. The results of this study suggest probiotic supplementation without anticoccidials can enhance performance and help alleviate the negative effects of a mixed Eimeria infection. ©2014 Poultry Science Association Inc.

JPRS Report, West Europe, Reference Aid, Glossary of Acronyms and Abbreviations of Italy

DTIC Science & Technology

1989-01-27

Italiana Naturisti bookseller bookshop Italian League for Divorce International League of Human Rights Italian Naturists League (i.e., nudists ...perpendiculari L.P.S. Lavori, Ponti e Strade l.r. lanciarazzi Italian Navy League National Naturists League (i.e., nudists ) Italian Origins Book

Assessing Regulatory Emotional Self-Efficacy in Three Countries

ERIC Educational Resources Information Center

Caprara, Gian Vittorio; Giunta, Laura Di; Eisenberg, Nancy; Gerbino, Maria; Pastorelli, Concetta; Tramontano, Carlo

2008-01-01

The Regulatory Emotional Self-Efficacy (RESE) scale was developed to assess perceived self-efficacy in managing negative (NEG) and in expressing positive (POS) affect (G. V. Caprara & M. Gerbino, 2001). In this study of young adults, the factorial structure of the RESE scale was found to be similar in Italy, the United States, and Bolivia: In…

ERP Responses to Violations in the Hierarchical Structure of Functional Categories in Japanese Verb Conjugation

ERIC Educational Resources Information Center

Kobayashi, Yuki; Sugioka, Yoko; Ito, Takane

2018-01-01

An event-related potential experiment was conducted in order to investigate readers' response to violations in the hierarchical structure of functional categories in Japanese, an agglutinative language where functional heads like Negation (Neg) as well as Tense (Tns) are realized as suffixes. A left-lateralized negativity followed by a P600 was…

76 FR 43639 - Takes of Marine Mammals Incidental to Specified Activities; Taking Marine Mammals Incidental to...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-07-21

... Operation of the Northeast Gateway Liquefied Natural Gas Port Facility in Massachusetts Bay AGENCY: National... application from Tetra Tech EC, Inc., on behalf of the Northeast Gateway[supreg] Energy Bridge TM L.P. (Northeast Gateway or NEG), for authorization to take marine mammals, by harassment, incidental to operating...

75 FR 42071 - Taking and Importing Marine Mammals; Operations of a Liquified Natural Gas Port Facility in...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-07-20

... was also published in the Federal Register for the proposed IHA for Northeast Gateway's LNG Port... Gateway Energy Bridge\\TM\\ L.L.C. (Northeast Gateway or NEG) and its partner, Algonquin Gas Transmission... its proposal to issue an authorization to Northeast Gateway and Algonquin to incidentally take, by...

USSR Report: Military Affairs, No. 1777, Communist of the Armed Forces

DTIC Science & Technology

1983-06-28

achieve significant successes in the socialist competition, to greet the 60th anni- versary of the founding of the USSR with new achievements in the...of the socialist economy. Rural and urban workers and fight- ingmen of the army and navy greeted the 60th anniversary of the founding of the USSR with...moment mentally to the sect. He recalled a certain incident. One of the visitors to the house of prayer had stuck his hand into the treasury. He soon

First SN Discoveries from the Dark Energy Survey

NASA Astrophysics Data System (ADS)

Abbott, T.; Abdalla, F.; Achitouv, I.; Ahn, E.; Aldering, G.; Allam, S.; Alonso, D.; Amara, A.; Annis, J.; Antonik, M.; Aragon-Salamanca, A.; Armstrong, R.; Ashall, C.; Asorey, J.; Bacon, D.; Balbinot, E.; Banerji, M.; Barbary, K.; Barkhouse, W.; Baruah, L.; Bauer, A.; Bechtol, K.; Becker, M.; Bender, R.; Benoist, C.; Benoit-Levy, A.; Bernardi, M.; Bernstein, G.; Bernstein, J. P.; Bernstein, R.; Bertin, E.; Beynon, E.; Bhattacharya, S.; Biesiadzinski, T.; Biswas, R.; Blake, C.; Bloom, J. S.; Bocquet, S.; Brandt, C.; Bridle, S.; Brooks, D.; Brown, P. J.; Brunner, R.; Buckley-Geer, E.; Burke, D.; Burkert, A.; Busha, M.; Campa, J.; Campbell, H.; Cane, R.; Capozzi, D.; Carlstrom, J.; Carnero Rosell, A.; Carollo, M.; Carrasco-Kind, M.; Carretero, J.; Carter, M.; Casas, R.; Castander, F. J.; Chen, Y.; Chiu, I.; Chue, C.; Clampitt, J.; Clerkin, L.; Cohn, J.; Colless, M.; Copeland, E.; Covarrubias, R. A.; Crittenden, R.; Crocce, M.; Cunha, C.; da Costa, L.; d'Andrea, C.; Das, S.; Das, R.; Davis, T. M.; Deb, S.; DePoy, D.; Derylo, G.; Desai, S.; de Simoni, F.; Devlin, M.; Diehl, H. T.; Dietrich, J.; Dodelson, S.; Doel, P.; Dolag, K.; Efstathiou, G.; Eifler, T.; Erickson, B.; Eriksen, M.; Estrada, J.; Etherington, J.; Evrard, A.; Farrens, S.; Fausti Neto, A.; Fernandez, E.; Ferreira, P. C.; Finley, D.; Fischer, J. A.; Flaugher, B.; Fosalba, P.; Frieman, J.; Furlanetto, C.; Garcia-Bellido, J.; Gaztanaga, E.; Gelman, M.; Gerdes, D.; Giannantonio, T.; Gilhool, S.; Gill, M.; Gladders, M.; Gladney, L.; Glazebrook, K.; Gray, M.; Gruen, D.; Gruendl, R.; Gupta, R.; Gutierrez, G.; Habib, S.; Hall, E.; Hansen, S.; Hao, J.; Heitmann, K.; Helsby, J.; Henderson, R.; Hennig, C.; High, W.; Hirsch, M.; Hoffmann, K.; Holhjem, K.; Honscheid, K.; Host, O.; Hoyle, B.; Hu, W.; Huff, E.; Huterer, D.; Jain, B.; James, D.; Jarvis, M.; Jarvis, M. J.; Jeltema, T.; Johnson, M.; Jouvel, S.; Kacprzak, T.; Karliner, I.; Katsaros, J.; Kent, S.; Kessler, R.; Kim, A.; Kim-Vy, T.; King, L.; Kirk, D.; Kochanek, C.; Kopp, M.; Koppenhoefer, J.; Kovacs, E.; Krause, E.; Kravtsov, A.; Kron, R.; Kuehn, K.; Kuemmel, M.; Kuhlmann, S.; Kunder, A.; Kuropatkin, N.; Kwan, J.; Lahav, O.; Leistedt, B.; Levi, M.; Lewis, P.; Liddle, A.; Lidman, C.; Lilly, S.; Lin, H.; Liu, J.; Lopez-Arenillas, C.; Lorenzon, W.; LoVerde, M.; Ma, Z.; Maartens, R.; Maccrann, N.; Macri, L.; Maia, M.; Makler, M.; Manera, M.; Maraston, C.; March, M.; Markovic, K.; Marriner, J.; Marshall, J.; Marshall, S.; Martini, P.; Marti Sanahuja, P.; Mayers, J.; McKay, T.; McMahon, R.; Melchior, P.; Merritt, K. W.; Merson, A.; Miller, C.; Miquel, R.; Mohr, J.; Moore, T.; Mortonson, M.; Mosher, J.; Mould, J.; Mukherjee, P.; Neilsen, E.; Ngeow, C.; Nichol, R.; Nidever, D.; Nord, B.; Nugent, P.; Ogando, R.; Old, L.; Olsen, J.; Ostrovski, F.; Paech, K.; Papadopoulos, A.; Papovich, C.; Patton, K.; Peacock, J.; Pellegrini, P. S. S.; Peoples, J.; Percival, W.; Perlmutter, S.; Petravick, D.; Plazas, A.; Ponce, R.; Poole, G.; Pope, A.; Refregier, A.; Reyes, R.; Ricker, P.; Roe, N.; Romer, K.; Roodman, A.; Rooney, P.; Ross, A.; Rowe, B.; Rozo, E.; Rykoff, E.; Sabiu, C.; Saglia, R.; Sako, M.; Sanchez, A.; Sanchez, C.; Sanchez, E.; Sanchez, J.; Santiago, B.; Saro, A.; Scarpine, V.; Schindler, R.; Schmidt, B. P.; Schmitt, R. L.; Schubnell, M.; Seitz, S.; Senger, R.; Sevilla, I.; Sharp, R.; Sheldon, E.; Sheth, R.; Smith, R. C.; Smith, M.; Snigula, J.; Soares-Santos, M.; Sobreira, F.; Song, J.; Soumagnac, M.; Spinka, H.; Stebbins, A.; Stoughton, C.; Suchyta, E.; Suhada, R.; Sullivan, M.; Sun, F.; Suntzeff, N.; Sutherland, W.; Swanson, M. E. C.; Sypniewski, A. J.; Szepietowski, R.; Talaga, R.; Tarle, G.; Tarrant, E.; Balan, S. Thaithara; Thaler, J.; Thomas, D.; Thomas, R. C.; Tucker, D.; Uddin, S. A.; Ural, S.; Vikram, V.; Voigt, L.; Walker, A. R.; Walker, T.; Wechsler, R.; Weinberg, D.; Weller, J.; Wester, W.; Wetzstein, M.; White, M.; Wilcox, H.; Wilman, D.; Yanny, B.; Young, J.; Zablocki, A.; Zenteno, A.; Zhang, Y.; Zuntz, J.

2012-12-01

The Dark Energy Survey (DES) report the discovery of the first set of supernovae (SN) from the project. Images were observed as part of the DES Science Verification phase using the newly-installed 570-Megapixel Dark Energy Camera on the CTIO Blanco 4-m telescope by observers J. Annis, E. Buckley-Geer, and H. Lin. SN observations are planned throughout the observing campaign on a regular cadence of 4-6 days in each of the ten 3-deg2 fields in the DES griz filters.

Topology, Structure and Functionality: Analysis, Modelling and Experimentation of Dense Granular Deformation in 2D and 3D

DTIC Science & Technology

2015-01-05

in a research position that will apply her skills at granular experiment and modeling to important issues related to pharmaceutical processing...MONITOR’S ACRONYM(S) (ES) ARO U.S. Anny Research Office 11 . SPONSOR/MONITOR’S REPORT P.O. Box 12211 NUMBER(S) Research Triangle Park, NC 27709-2211...decision, unless so designated by other documentation. 14. ABSTRACT This project, j oint with Antoinette Tordesillas ofUniversity of Melbomn e

Why Throw the Negs out with the Bathwater? A Study of Students' Attitudes to Digital and Film Photographic Media

ERIC Educational Resources Information Center

Macdonald, Iain

2012-01-01

As today's digital applications hold our gaze and become increasingly ubiquitous, it is easy to dismiss the previous technologies and processes that provided yesterday's creative opportunities. Photography has been revolutionised by digital capture and transmission in the past decade. It could be argued that there is a digital orthodoxy in…

Ambiguous dependence of fluorescence intensity of trees on chlorophyll concentration

NASA Astrophysics Data System (ADS)

Zavoruev, Valeriy V.; Zavorueva, Elena N.

2014-11-01

Using fluorimetry Junior PAM (Heinz Walz GmbH, Germany) fluorescence parameters of leaves Prinsepia sinensis, Crataegus chlorocarca M, Acer negúndo, Bétula péndula are studied. It was found that the dependence of maximum fluorescence (Fm) plants on the concentration of chlorophyll depends on the sampling method during of vegetation. The correctness of sampling proves during vegetation is substantiated.

Remoteness Equals Backwardness? Human Capital and Market Access in the European Regions: Insights from the Long Run

ERIC Educational Resources Information Center

Diebolt, Claude; Hippe, Ralph

2018-01-01

In a recent contribution, Redding and Schott [2003. "Distance, Skill Deepening and Development: Will Peripheral Countries Ever Get Rich?" "Journal of Development Economics": 72 (2): 515-541. doi:10.1016/S0304-3878(03)00118-4] add human capital to a two sector NEG model, highlighting that remoteness represents a penalty that…

Asymmetric B-factory note

DOE Office of Scientific and Technical Information (OSTI.GOV)

Calderon, M.

Three main issues giving purpose to our visit to CERN, ESRF and DESY were to: assess the current thinking at CERN on whether Eta, the gas desorption coefficient, would continue to decrease with continued with continued beam cleaning, determine if the time between NEG reconditioning could be expanded, and acquire a knowledge of the basic fabrication processes and techniques for producing beam vacuum chambers of copper.

Algorithm Development for a Real-Time Military Noise Monitor

DTIC Science & Technology

2006-03-24

Duration ESLM Enhanced Sound Level Meter ERDC-CERL Engineer Research and Development Center/Construction Engineering Research Laboratory FFT...Fast Fourier Transform FTIG Fort Indiantown Gap Kurt Kurtosis LD Larson Davis Leq Equivalent Sound Level L8eq 8-hr Equivalent...Sound Level Lpk Peak Sound Level m Spectral Slope MCBCL Marine Corps Base Camp Lejeune Neg Number of negative samples NI National

78 FR 12306 - Combined Notice of Filings

Federal Register 2010, 2011, 2012, 2013, 2014

2013-02-22

... effective 4/1/2017. Filed Date: 2/12/13. Accession Number: 20130212-5025. Comments Due: 5 p.m. e.t. 2/25/13... Secretary [FR Doc. 2013-04076 Filed 2-21-13; 8:45 am] BILLING CODE 6717-01-P .... Description: Duke Energy Carolinas 4-1-2013 Neg Rate to be effective 4/1/2013. Filed Date: 2/12/13. Accession...

Robust high pressure stability and negative thermal expansion in sodium-rich antiperovskites Na 3OBr and Na 4OI 2

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wang, Yonggang; Wen, Ting; Park, Changyong

2016-01-14

The structure stability under high pressure and thermal expansion behavior of Na 3OBr and Na 4OI 2, two prototypes of alkali-metal-rich antiperovskites, were investigated by in situ synchrotron X-ray diffraction techniques under high pressure and low temp. Both are soft materials with bulk modulus of 58.6 GPa and 52.0 GPa for Na 3OBr and Na 4OI 2, resp. The cubic Na 3OBr structure and tetragonal Na 4OI 2 with intergrowth K 2NiF 4 structure are stable under high pressure up to 23 GPa. Although being a characteristic layered structure, Na 4OI 2 exhibits nearly isotropic compressibility. Neg. thermal expansion wasmore » obsd. at low temp. range (20-80 K) in both transition-metal-free antiperovskites for the first time. The robust high pressure structure stability was examined. and confirmed by first-principles calculations. among various possible polymorphisms qualitatively. The results provide in-depth understanding of the neg. thermal expansion and robust crystal structure stability of these antiperovskite systems and their potential applications.« less

Microbeam radiosurgery: An industrial perspective.

PubMed

Wright, Michael D

2015-09-01

In spite of its long demonstrated potential, microbeam radiosurgery (MBRS) has yet to be developed into a clinical tool. This article examines the problems associated with MBRS, and potential solutions. It is shown that a path to a clinically useful device is emerging. Copyright © 2015 Associazione Italiana di Fisica Medica. Published by Elsevier Ltd. All rights reserved.

The Emergence of For-Profit E-learning Providers in Asia

ERIC Educational Resources Information Center

Perkins, Ross

2009-01-01

This article presents an interview with Dr. Luca Botturi, winner of the 2008 Educational Communication and Technology (ECT) Foundation Robert deKeiffer International Fellowship Award. Dr. Botturi, an Italian originally from the suburbs of Milano, works at the Universita della Svizzera italiana (USI, also called the University of Lugano) in its New…

Interdisciplinary Co-operation (Part II of "Language Learning: Individual Needs, Interdisciplinary Co-operation, Bi- and Multilingualism").

ERIC Educational Resources Information Center

1978

The following papers on interdisciplinary cooperation in second language instruction are included: (1) "Language Teaching: Possibilities for Interdisciplinary Co-operation," by James E. Alatis; (2) "L'insegnamento della letteratura italiana (The Teaching of Italian Literature)," by Ezio Raimondi; (3) "Objective Evaluation and Transparency," by…

The AISPEP (Associazione Italiana Sindromi Pelvico Prostatiche) chronic prostatitis questionnaire (AISPEP-Q). focus on the disease: anamnestic data, life activities, symptoms, sexual habits, quality of life and knowledge about prostatitis from 93 questions answered on the Internet.

PubMed

Mazzoli, Sandra; Magri, Vittorio; Guercini, Federico; Simone, Alberto; Paolicchi, Fabio; Cai, Tommaso

2007-06-01

Chronic prostatitis (CP) has been described as one of the most common illnesses men aged < or = 50, showing a significant impact on patients' quality of life comparable with other chronic diseases, such as unstable angina or Crohn's disease. CP also is a social and economic problem due to its high incidence in the young male population and to the absence of evidence for the effectiveness of treatment. Today, however, although validated outcome questionnaires are available to follow prostatitis patients, diagnostic and treatment options are based on experience, expert opinion and poor clinical trial data. More extensive and better-designed epidemiological studies are needed to evaluate and describe prostatitis patient clinical characteristics, in order to carry out correct and useful treatment. The aim of this report is to present the new Associazione Italiana Sindromi Pelvico Prostatiche questionnaire (AISPEP-Q) in order to provide a tool for increasing knowledge in prostatitis patient characteristics and design future epidemiological studies.

Injectable trace minerals (selenium, copper, zinc, and manganese) alleviates inflammation and oxidative stress during an aflatoxin challenge in lactating multiparous Holstein cows.

PubMed

Pate, R T; Cardoso, F C

2018-06-20

Trace minerals are vital in the antioxidant response during oxidative stress; however, limited research is available on the effects of trace mineral supplementation during an aflatoxin (AF) challenge. The objective of the study was to determine the effects of 2 subcutaneous injections of 15 mg/mL of Cu, 5 mg/mL of Se, 60 mg/mL of Zn, and 10 mg/mL of Mn (Multimin 90, Multimin North America, Fort Collins, CO) given at 1 mL/90.7 kg of average body weight in response to an AF challenge. Fifty-eight Holstein cows [body weight (mean ± SD) = 734 ± 6 0kg; days in milk = 191 ± 93] were assigned to 1 of 3 treatments in a randomized complete block design. The experimental period (63 d) was divided into an adaptation phase (d 1-56) and a measurement phase (d 57-63). From d 57 to 59, cows received an AF challenge that consisted of 100 μg of aflatoxin B 1 /kg of dietary dry matter intake (DMI) administered orally via balling gun. Treatments were saline injection and no AF challenge (NEG), saline injection and AF challenge (POS), and trace mineral injection and AF challenge (MM). Injections were performed subcutaneously on d 1 and 29. Milk was sampled 3 times daily from d 56 to 63, blood was sampled on d 0, 56, 60, and 63, and liver samples were taken on d 0 and 60. Two treatment orthogonal contrasts [CONT1 (NEG vs. POS) and CONT2 (POS vs. MM)] were made. Cows in NEG had lower AF excretion in milk and greater 3.5% fat-corrected milk (32.1 ± 1.37 kg/d) compared with cows in POS (28.6 ± 1.43 kg/d). Feed efficiencies (3.5% fat-corrected milk/DMI, energy-corrected milk/DMI, and milk/DMI) were greater for cows in NEG (1.42 ± 0.07, 1.46 ± 0.07, and 1.45 ± 0.07, respectively) than cows in POS (1.16 ± 0.08, 1.18 ± 0.08, and 1.22 ± 0.07, respectively). Cows in POS had greater milk urea nitrogen and blood urea nitrogen than cows in MM. Liver concentrations of Se and Fe were greater for cows in MM compared with cows in POS. Cows in MM tended to have greater plasma glutathione peroxidase activity compared with cows in POS. An upregulation of liver GPX1 was observed for cows in POS compared with cows in MM. In conclusion, subcutaneous injection of trace minerals maintained an adequate antioxidant response when an AF challenge was present. Copyright © 2018 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

8. EARLY PHOTO OF THE CABIN WITH DOG TROT SECOND ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

8. EARLY PHOTO OF THE CABIN WITH DOG TROT SECOND PEN AND CHIMNEY, PORCH, STEPS AND COMPOSITION ROOF. J. T. Young Jr., Annie Ruth Young, Bonnie Marie Young and Nadine Young, relatives of the photograph's donor, appear in the foreground. The structure in front of the house and to the right of the tree is a cage for pet squirrels. 2-1/4 x 2-1/4 copy negative, courtesy of former resident Preston Young. Photographer unknown, 1923. - Thomas Jefferson Walling Log Cabin, Henderson, Rusk County, TX

Queerer than queer: reflections of a Kike dyke.

PubMed

Goldflam, A

1999-01-01

In this article the author, a Jewish lesbian academic, reflects on the positive and negative aspects of her dual identity and explores her feelings of exclusion from "in groups" in a range of settings. Annie Goldflam carries a legacy of fear from stories of medieval "witch" burnings and drownings and the twentieth century Jewish Holocaust, which she reflects on to compare the nature of anti-semitism and lesbophobia, considering the options of hiding from racist and homophobic forms of oppression or confronting them. She has chosen to confront, be "out" and politically active.

Nonalignment To Balance Chinas Influence On Sri Lanka: Negotiating Chinas String Of Pearls Strategy For The Pearl Of The Indian Ocean

DTIC Science & Technology

2017-12-01

crisis-is-so-bad-the-government- doesnt-even-know-how-much- money -it-owes/#c6eb6a946082. 3 Ibid. 4 Ibid. 5 Iromi Dharmawardana, Sri Lanka Foreign...Annie Gowen, “Can Sri Lanka’s New Government Break Free from China,” Washington Post, August 16, 2015, http://www.washingtonpost.com/world...55 Wade Shepard, “China Tells Sri Lanka: We Want Our Money , Not Your Empty Port,” Forbes, July 31, 2016, http://www.forbes.com

Reading on Grade Level in Third Grade: How Is It Related to High School Performance and College Enrollment? A Longitudinal Analysis of Third-Grade Students in Chicago in 1996-97 and Their Educational Outcomes. A Report to the Annie E. Casey Foundation

ERIC Educational Resources Information Center

Lesnick, Joy; Goerge, Robert M.; Smithgall, Cheryl; Gwynne, Julia

2010-01-01

Learning to read is one of the most important skills in modern society. Not only does reading serve as the major foundational skill for school-based learning, but reading ability is strongly related to opportunities for academic and vocational success. For children, a critical transition takes place during elementary school: until the end of third…

Biological and Behavioral Response Studies of Marine Mammals in Southern California, 2011 (SOCAL-11)

DTIC Science & Technology

2012-09-01

Hildebrand, C. Kyburg, R. Carlson, T. Yack, and J. Barlow ) EXECUTIVE SUMMARY PROJECT OBJECTIVES METHODOLOGY AND FOCAL SPECIES SOCAL-11...HYDROPHONE ARRAY. ( Tina M. Yack, Shannon Coates, Jay Barlow , John Calambokidis, Annie Douglas, and Shannon Rankin) INTRODUCTION MATERIALS AND...E. Falcone, G. Schorr, A. Douglas,  A. Stimpert, J. Hildebrand, C. Kyburg, R. Carlson, T. Yack, J.  Barlow        SOCAL‐11 PROJECT REPORT  2

Photocopy of photograph (original photograph located in the Hawaii State ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of photograph (original photograph located in the Hawaii State Archives, Admiral Furlong Collection), HAS Neg. 199.016. U.S. Navy photograph, August 21, 1919. MRS. JOSEPHUS DANIELS, WIFE OF THE SECRETARY OF THE NAVY, PUSHED A BUTTON AND DRYDOCK NO. 1 (FACILITY S779) WAS OFFICIALLY INAUGURATED. - U.S. Naval Base, Pearl Harbor, Waterfront Facilities, Various locations throughout base, Pearl City, Honolulu County, HI

JPRS Report, East Europe.

DTIC Science & Technology

1988-10-28

Czechoslovak people is closely connected with the question whether the revisionist forces will succeed in penetrating further and deeper into...any values originating in our society precisely because they originated in it. The neg- ativism of these people is like a roundabout reflection of... people knew more about these matters, there would be fewer illusions concerning those periods. And then, less would be said about a multiparty system

Violent crime and substance abuse: a medico-legal comparison between deceased users of anabolic androgenic steroids and abusers of illicit drugs.

PubMed

Klötz, Fia; Petersson, Anna; Isacson, Dag; Thiblin, Ingemar

2007-11-15

Several case reports and survey studies have indicated that abuse of anabolic androgenic steroids (AAS) often leads to increased aggressiveness and feelings of hostility that may occasionally trigger violent behaviour. Other observations indicate that many users of AAS also abuse alcohol and/or various illegal substances. Since substance abuse is a well-known risk factor for violent behaviour, it could be that violence committed by AAS users might, at least in many cases, actually be caused by abuse of other drugs. In order to examine this possibility further here, the criminal histories (in terms of incidences of convictions) of deceased users of AAS with (AASpos-subst.pos) and without (AASpos-subst.neg) signs of abuse of other illegal substances were compared to the corresponding histories of deceased users of illicit substances testing negatively for AAS (subst.pos-AASneg) at the time of autopsy. The risk of being convicted for a crime against property was significantly higher in the subst.pos-AASneg group than in either the AASpos-subst.neg or AASpos-subst.pos groups (RR=0.048 versus 0.408). At the same time, the risk of being convicted for a crime of violence was at least as high for the two AAS-positive groups as for the AAS-negative group. Furthermore, when compared with the first 3 years after the first criminal conviction, a pronounced increase in the proportion of incidence of violent crimes and a marked reduction in the proportion of incidence of crime against property was observed during the 3-year period immediately preceding death only among the AASpos-subst.neg subjects. In conclusion, the incidence of violent crime among users of AAS without signs of other drug abuse was comparable to the corresponding incidences for drug addicts without AAS use. This observation suggests that the violent criminality observed among AAS users is not confounded in any systematic fashion by abuse of other drugs. The findings also indicate that use of AAS in certain predisposed individuals might cause a high rate of violent crimes, especially if the use of AAS is combined with the use of other illegal substances.

Liposuction Preserves the Morphological Integrity of the Microvascular Network: Flow Cytometry and Confocal Microscopy Evidence in a Controlled Study.

PubMed

Bertheuil, Nicolas; Chaput, Benoit; Berger-Müller, Sandra; Ménard, Cédric; Mourcin, Frédéric; Watier, Eric; Grolleau, Jean-Louis; Garrido, Ignacio; Tarte, Karin; Sensébé, Luc; Varin, Audrey

2016-05-01

Liposuction is a very popular technique in plastic surgery that allows for the taking adipose tissue (AT) on large surfaces with little risk of morbidity. Although liposuction was previously shown to preserve large perforator vessels, little is known about the effects of liposuction on the microvasculature network. The aim of this study was to analyze the effect of liposuction on the preservation of microvessels at tissue and cellular levels by flow cytometry and confocal microscopy following abdominoplasty procedure. Percentage of endothelial cells in AT from liposuction and en bloc AT was determined by multicolor flow cytometry. Moreover, vessel density and adipocyte content were analyzed in situ in 3 different types of AT (en bloc, from liposuction, and residual AT after liposuction) by confocal microscopy. Flow cytometric analysis showed that en bloc AT contained 30.6% ± 12.9% and AT from liposuction 21.6% ± 9.9% of endothelial cells (CD31(pos)/CD45(neg)/CD235a(neg)/CD11b(neg)) (P = .009). Moreover, analysis of paired AT from the same patients (n = 5) confirmed a lower percentage of endothelial cells in AT from liposuction compared to en bloc AT (17.7% ± 4.5% vs 21.9% ± 3.3%, P = .031). Likewise, confocal microscopy showed that en bloc AT contained 8.2% ± 6.3%, AT from liposuction only 1.6% ± 1.0% (P < .0001), and AT after liposuction 8.9% ± 4.1% (P = .111) of CD31(pos) vessels. Conversely, adipocyte content was 39.5% ± 14.5% in the en bloc AT, 45% ± 18.4% in AT from liposuction (P = .390), and 18.8 ± 14.8% in AT after liposuction (P = .011). For the first time, we demonstrate that liposuction preserves the microvascular network. Indeed, a low percentage of endothelial cells was found in AT from liposuction and we confirm the persistence of microvessels in the tissue after liposuction. © 2015 The American Society for Aesthetic Plastic Surgery, Inc. Reprints and permission: journals.permissions@oup.com.

Infusion of freshly isolated autologous bone marrow derived mononuclear cells prevents endotoxin-induced lung injury in an ex-vivo perfused swine model

PubMed Central

2013-01-01

Introduction The acute respiratory distress syndrome (ARDS), affects up to 150,000 patients per year in the United States. We and other groups have demonstrated that bone marrow derived mesenchymal stromal stem cells prevent ARDS induced by systemic and local administration of endotoxin (lipopolysaccharide (LPS)) in mice. Methods A study was undertaken to determine the effects of the diverse populations of bone marrow derived cells on the pathophysiology of ARDS, using a unique ex-vivo swine preparation, in which only the ventilated lung and the liver are perfused with autologous blood. Six experimental groups were designated as: 1) endotoxin alone, 2) endotoxin + total fresh whole bone marrow nuclear cells (BMC), 3) endotoxin + non-hematopoietic bone marrow cells (CD45 neg), 4) endotoxin + hematopoietic bone marrow cells (CD45 positive), 5) endotoxin + buffy coat and 6) endotoxin + in vitro expanded swine CD45 negative adherent allogeneic bone marrow cells (cultured CD45neg). We measured at different levels the biological consequences of the infusion of the different subsets of cells. The measured parameters were: pulmonary vascular resistance (PVR), gas exchange (PO2), lung edema (lung wet/dry weight), gene expression and serum concentrations of the pro-inflammatory cytokines IL-1β, TNF-α and IL-6. Results Infusion of freshly purified autologous total BMCs, as well as non-hematopoietic CD45(-) bone marrow cells significantly reduced endotoxin-induced pulmonary hypertension and hypoxemia and reduced the lung edema. Also, in the groups that received BMCs and cultured CD45neg we observed a decrease in the levels of IL-1β and TNF-α in plasma. Infusion of hematopoietic CD45(+) bone marrow cells or peripheral blood buffy coat cells did not protect against LPS-induced lung injury. Conclusions We conclude that infusion of freshly isolated autologous whole bone marrow cells and the subset of non-hematopoietic cells can suppress the acute humoral and physiologic responses induced by endotoxemia by modulating the inflammatory response, mechanisms that do not involve engraftment or trans-differentiation of the cells. These observations may have important implications for the design of future cell therapies for ARDS. PMID:23497755

The Community as a Source of Pragmatic Input for Learners of Italian: The Multimedia Repository LIRA

ERIC Educational Resources Information Center

Zanoni, Greta

2016-01-01

This paper focuses on community participation within the LIRA project--Lingua/Cultura Italiana in Rete per l'Apprendimento (Italian language and culture for online learning). LIRA is a multimedia repository of e-learning materials aiming at recovering, preserving and developing the linguistic, pragmatic and cultural competences of second and third…

[Digoxin poisoning: new prospects for therapy].

PubMed

Marino, Alfonsina; Giordano, Lorenzo; Ardu, Francesco

2017-04-01

The filter has been approved by the Food and Drug Administration for the removal of beta-2 microglobulin in patient undergoing hemodialysis. We used the filter (the patient agrees) off label, in the course of digitalis intoxication and we have shown that the filter is capable of removing the drug effectively. Copyright by Società Italiana di Nefrologia SIN, Rome, Italy.

Internet-Based Cervical Cytology Screening Program

DTIC Science & Technology

2005-04-01

and Second Trials of Each Pathologist. * Reference diagnostic category: NEG, ASCUS , LSIL > HSIL Pathologist 1 Interpretation. Negative or LSIL 24 (22...Principal Investigator: David C. Wdlbbur, M.D. Description of Subject Population: Women above the age of l18 years having Pap tests performe PURPOSE We...Subject Population: Women above the age of 18 years having Pay tests performed IRB Protocol Number: 2003P-001658 Sponsor Protocol Number: n/a Consent Form

Massive plasmablast response elicited in the acute phase of hantavirus pulmonary syndrome.

PubMed

García, Marina; Iglesias, Ayelén; Landoni, Verónica I; Bellomo, Carla; Bruno, Agostina; Córdoba, María Teresa; Balboa, Luciana; Fernández, Gabriela C; Sasiain, María Del Carmen; Martínez, Valeria P; Schierloh, Pablo

2017-05-01

Beside its key diagnostic value, the humoral immune response is thought to play a protective role in hantavirus pulmonary syndrome. However, little is known about the cell source of these antibodies during ongoing human infection. Herein we characterized B-cell subsets circulating in Andes-virus-infected patients. A notable potent plasmablast (PB) response that increased 100-fold over the baseline levels was observed around 1 week after the onset of symptoms. These PB present a CD3 neg CD19 low CD20 neg CD38 hi CD27 hi CD138 +/- IgA +/- surface phenotype together with the presence of cytoplasmic functional immunoglobulins. They are large lymphocytes (lymphoblasts) morphologically coincident with the 'immunoblast-like' cells that have been previously described during blood cytology examinations of hantavirus-infected patients. Immunoreactivity analysis of white blood cell lysates suggests that some circulating PB are virus-specific but we also observed a significant increase of reactivity against virus-unrelated antigens, which suggests a possible bystander effect by polyclonal B-cell activation. The presence of this large and transient PB response raises the question as to whether these cells might have a protective or pathological role during the ongoing hantavirus pulmonary syndrome and suggest their practical application as a diagnostic/prognostic biomarker. © 2017 John Wiley & Sons Ltd.

HTLV-1 Tax-mediated inhibition of FOXO3a activity is critical for the persistence of terminally differentiated CD4+ T cells.

PubMed

Olagnier, David; Sze, Alexandre; Bel Hadj, Samar; Chiang, Cindy; Steel, Courtney; Han, Xiaoying; Routy, Jean-Pierre; Lin, Rongtuan; Hiscott, John; van Grevenynghe, Julien

2014-12-01

The mechanisms involved in the persistence of activated CD4+ T lymphocytes following primary human T leukemia/lymphoma virus type 1 (HTLV-1) infection remain unclear. Here, we demonstrate that the HTLV-1 Tax oncoprotein modulates phosphorylation and transcriptional activity of the FOXO3a transcription factor, via upstream activation of the AKT pathway. De novo HTLV-1 infection of CD4+ T cells or direct lentiviral-mediated introduction of Tax led to AKT activation and AKT-dependent inactivation of FOXO3a, via phosphorylation of residues Ser253 and Thr32. Inhibition of FOXO3a signalling led to the long-term survival of a population of highly activated, terminally differentiated CD4+Tax+CD27negCCR7neg T cells that maintained the capacity to disseminate infectious HTLV-1. CD4+ T cell persistence was reversed by chemical inhibition of AKT activity, lentiviral-mediated expression of a dominant-negative form of FOXO3a or by specific small interfering RNA (siRNA)-mediated silencing of FOXO3a. Overall this study provides new mechanistic insight into the strategies used by HTLV-1 to increase long-term maintenance of Tax+CD4+ T lymphocytes during the early stages of HTLV-1 pathogenesis.

Social Pavlovian conditioning: Short- and long-term effects and the role of anxiety and depressive symptoms

PubMed Central

Wilhelm, Frank H.; Boger, Sabrina; Georgii, Claudio; Klimesch, Wolfgang; Blechert, Jens

2017-01-01

Abstract Today’s stressors largely arise from social interactions rather than from physical threat. However, the dominant laboratory model of emotional learning relies on physical stimuli (e.g. electric shock) whereas adequate models of social conditioning are missing, possibly due to more subtle and multilayered biobehavioral responses to such stimuli. To fill this gap, we acquired a broad set of measures during conditioning to negative social unconditioned stimuli, also taking into account long-term maintenance of conditioning and inter-individual differences. Fifty-nine healthy participants underwent a classical conditioning task with videos of actors expressing disapproving (US-neg) or neutral (US-neu) statements. Static images of the corresponding actors with a neutral facial expression served as CS+ and CS−, predicting US-neg and US-neu, respectively. Autonomic and facial-muscular measures confirmed differential unconditioned responding whereas experiential CS ratings, event-related potentials, and evoked theta oscillations confirmed differential conditioned responding. Conditioning was maintained at 1 month and 1 year follow-ups on experiential ratings, especially in individuals with elevated anxiety and depressive symptoms, documenting the efficiency of social conditioning and its clinical relevance. This novel, ecologically improved conditioning paradigm uncovered a remarkably efficient multi-layered social learning mechanism that may represent a risk factor for anxiety and depression. PMID:27614767

HTLV-1 Tax-Mediated Inhibition of FOXO3a Activity Is Critical for the Persistence of Terminally Differentiated CD4+ T Cells

PubMed Central

Bel Hadj, Samar; Chiang, Cindy; Steel, Courtney; Han, Xiaoying; Routy, Jean-Pierre; Lin, Rongtuan; Hiscott, John; van Grevenynghe, Julien

2014-01-01

The mechanisms involved in the persistence of activated CD4+ T lymphocytes following primary human T leukemia/lymphoma virus type 1 (HTLV-1) infection remain unclear. Here, we demonstrate that the HTLV-1 Tax oncoprotein modulates phosphorylation and transcriptional activity of the FOXO3a transcription factor, via upstream activation of the AKT pathway. De novo HTLV-1 infection of CD4+ T cells or direct lentiviral-mediated introduction of Tax led to AKT activation and AKT-dependent inactivation of FOXO3a, via phosphorylation of residues Ser253 and Thr32. Inhibition of FOXO3a signalling led to the long-term survival of a population of highly activated, terminally differentiated CD4+Tax+CD27negCCR7neg T cells that maintained the capacity to disseminate infectious HTLV-1. CD4+ T cell persistence was reversed by chemical inhibition of AKT activity, lentiviral-mediated expression of a dominant-negative form of FOXO3a or by specific small interfering RNA (siRNA)-mediated silencing of FOXO3a. Overall this study provides new mechanistic insight into the strategies used by HTLV-1 to increase long-term maintenance of Tax+CD4+ T lymphocytes during the early stages of HTLV-1 pathogenesis. PMID:25521510

Water-quality characteristics for selected streams in Lawrence County, South Dakota, 1988-92

USGS Publications Warehouse

Williamson, Joyce E.; Hayes, Timothy Scott

2000-01-01

During the 1980?s, significant economic development and population growth began to occur in Lawrence County in the northern part of the Black Hills of western South Dakota. Rising gold prices and heap-leach extraction methods allowed the economic recovery of marginal gold ore deposits, resulting in development of several large-scale, open-pit gold mines in Lawrence County. There was increasing local concern regarding potential impacts on the hydrologic system, especially relating to the quantity and quality of water in the numerous streams and springs of Lawrence County. In order to characterize the water quality of selected streams within Lawrence County, samples were collected from 1988 through 1992 at different times of the year and under variable hydrologic conditions. During the time of this study, the Black Hills area was experiencing a drought; thus, most samples were collected during low-flow conditions.Streamflow and water-quality characteristics in Lawrence County are affected by both geologic conditions and precipitation patterns. Most streams that cross outcrops of the Madison Limestone and Minnelusa Formation lose all or large part of their streamflow to aquifer recharge. Streams that are predominantly spring fed have relatively stable streamflow, varying slightly with dry and wet precipitation cycles.Most streams in Lawrence County generally have calcium magnesium bicarbonate type waters. The sites from the mineralized area of central Lawrence County vary slightly from other streams in Lawrence County by having higher concentrations of sodium, less bicarbonate, and more sulfate. False Bottom Creek near Central City has more sulfate than bicarbonate. Nitrogen, phosphorous, and cyanide concentrations were at or near the laboratory reporting limits for most sites and did not exceed any of the water-quality standards. Nitrite plus nitrate concentrations at Annie Creek near Lead, Whitetail Creek at Lead, Squaw Creek near Spearfish, and Spearfish Creek below Robison Gulch were somewhat higher than at other sites. Mining activity, agricultural activity, and domestic development are possible sources of nitrogen to the streams. Increased mining activities were identified as the probable cause of increased nitrogen concentrations in Annie Creek.In the mineralized area of the northern Black Hills, detectable concentrations of trace elements are common in stream water, occasionally exceeding beneficial-use and aquatic-life criteria. In addition, many basins have been disturbed by both historical and recent mining operations and cleanup activities. The maximum dissolved arsenic concentration at Annie Creek near Lead (48 micrograms per liter) approached the current arsenic drinking-water standard. Concentrations at or greater than 5 micrograms per liter were found in samples from Annie Creek near Lead, Spearfish Creek above Spearfish, Whitetail Creek at Lead, and False Bottom Creek near Spearfish. Bear Butte Creek near Deadwood had one sample with a dissolved copper concentration that exceeded acute and chronic aquatic-life criteria. Bear Butte Creek near Deadwood had several manganese concentrations that exceeded the secondary maximum contaminant level of 50 micrograms per liter.Bed-sediment and water-quality data from selected sites in small drainage basins were used to determine if factors such as pH, arsenic concentrations in bed sediments, and calcite saturation control dissolved arsenic concentrations. Arsenic solubility is controlled by adsorption, mainly on ferrihydrite. In addition, adsorption/desorption of arsenic is controlled by the pH of the stream, with high arsenic concentrations appearing only at higher pH conditions (above 8). There are significant arsenic sources available to almost all the small streams of the northern Black Hills mining area, but arsenic is less mobile in streams that are not influenced to the higher pH values by calcite. Streams where arsenic is more mobile have lower iron concentrations i

Archaeological Survey 31BF115 and 31BF117. Phase 1. Archaeological Survey 31BF115. Phase 2. Texasgulf, Bath Creek, North Carolina

DTIC Science & Technology

1987-03-01

wrote of Bath Town that it had "deeply rooted adultery, incest , blasphemy, and all kinds of profaneness (Broadwater et al 1979)." By 1755, the...Sarah, age 48, and children William (16), John (14), Thomas (12), Liney (10), and Annie (6). Thomas was a farmer and his three oldest sons assisted him on...to his son , R. H. Archbell, for $1000.00 (Deed Book 247:241). It included ’all the stock, team, and farm equipment on the above farm known as the

Sponsor gnomonici di 2000 anni fa nelle Dolomiti e a Pompei

NASA Astrophysics Data System (ADS)

del Favero, Enrico

2003-01-01

The author describes two memorial tablets of the Roman age, probably dating back to the first Century AD, which were discovered in Belluno province, in the towns of Valle di Cadore and Castellavazzo. They are concerned with sundials donated to the local communities by coeval sponsors. After brief recalling how people measured the time during the Roman age, the meaning of a similar tablet found in Pompeii is examined. This last tablet is included - inter alia - in the Michael T. Bragg's website on Pompeii, which has been found thanks to the so-called Roth's sundials list.

The Fifth Data Release of the Sloan Digital Sky Survey

DTIC Science & Technology

2007-10-01

Gilmore,10 Karl Glazebrook,8 Jim Gray,24 Eva K . Grebel,25 James E. Gunn,5 Ernst de Haas,5 Patrick B . Hall,26 Michael Harvanek,4 Suzanne L. Hawley,2...THE FIFTH DATA RELEASE OF THE SLOAN DIGITAL SKY SURVEY Jennifer K . Adelman-McCarthy,1 Marcel A. Agüeros,2 Sahar S. Allam,1,3 Kurt S. J. Anderson,4...Scott F. Anderson,2 James Annis,1 Neta A. Bahcall,5 Coryn A. L. Bailer-Jones,6 Ivan K . Baldry,7,8 J. C. Barentine,4 Timothy C. Beers,9 V. Belokurov,10

"Tugboat Annie:" nursing's hero of Pearl Harbor--Grace Lally (1897-1983).

PubMed

Hawkins, J W; Matthews, I

1991-01-01

In 1991 we will mark the 50th anniversary of the Japanese attack on Pearl Harbor. Few nurses know that the Chief Nurse aboard the USS Solace, the only hospital ship in port on that fateful day, played a critical role in caring for the survivors. Grace Lally's calm, professional manner, along with her sensitivity to the needs of her patients, her 12 nurses, and the crew of the Solace made her a hero. This study is an investigation of her role on December 7, 1941, and her contributions as a career military nurse.

Studies in Intelligence. Volume 51, Number 2, 2007

DTIC Science & Technology

2007-01-01

in the United States, the routine interactions of trade and transnational business and finance , and the exchange of hun- dreds of thousands of...he sought cordial relations with the United States in order to obtain financing for major devel- opment projects, notably the Aswan High Dam. He...expert on Middle East affairs and knowl- edgeable in international finance . Hoover did not share Dulles’s neg- ative view of Nasser and played a key

The LIS Corpus Project: A Discussion of Sociolinguistic Variation in the Lexicon

ERIC Educational Resources Information Center

Geraci, Carlo; Battaglia, Katia; Cardinaletti, Anna; Cecchetto, Carlo; Donati, Caterina; Giudice, Serena; Mereghetti, Emiliano

2011-01-01

Following a well-established tradition going back to the 1980s (cf. Volterra 1987/2004), the authors use the name Lingua dei Segni Italiana (Italian Sign Language [LIS]) for the language used by Italian deaf people (and by Swiss deaf people living in the Ticino canton). LIS is becoming more and more visible, and its status as a minority language…

Curriculum Guide for Italian Language Arts. Literature Levels AB = Guida alla Lettura della Lingua Italiana Letteratura Ciclo A & B. Working Draft.

ERIC Educational Resources Information Center

Chicago Board of Education, IL. Dept. of Curriculum.

A curriculum and teaching guide for introductory literature appreciation in the Chicago public schools is an orientation for the bilingual teacher of Italian students. Concepts such as rhyme and personification are developed for the teacher. For each of the two levels included, the section begins with a list of specific performance objectives,…

Attivita del Centro di Linguistica Applicata nel campo della lingua e della cultura italiana all'estero (Activities of the Italian Center for Applied Linguistics concerning Italian Language and Culture Abroad).

ERIC Educational Resources Information Center

Boriosi Katerinov, Maria Clotilde

1979-01-01

Outlines the activities of a recently created section of the Italian Center for Applied Linguistics (CILA), dealing with "Teaching Italian Abroad." Describes these activities as encompassing four areas: research, teaching methodology, consultation, teachers' training and bibliographical information. Lists statistical surveys, research papers, and…

1991-1992 Europe & Asia in Space.

DTIC Science & Technology

1991-01-01

being designed is the SICRAL ( Sistema Italiana de Communicazione Riservente Allarmi) military communications systems with satellites posi- tioned...3,053 km orbit. Using a concept very similar to Aktivnyy, the Czech Magion-3 is a companion to the mother satellite. The mother craft injects...Magion spacecraft have been successfully deployed since the first in 1978 with Interkosmos 18. The second and third in the series were companions

Acute phase response in lactating dairy cows during hyperinsulinemic hypoglycaemic and hyperinsulinemic euglycaemic clamps and after intramammary LPS challenge.

PubMed

De Matteis, L; Bertoni, G; Lombardelli, R; Wellnitz, O; Van Dorland, H A; Vernay, M C M B; Bruckmaier, R M; Trevisi, E

2017-06-01

The link between energy availability, turnover of energy substrates and the onset of inflammation in dairy cows is complex and poorly investigated. To clarify this, plasma inflammatory variables were measured in mid-lactating dairy cows allocated to three groups: hyperinsulinemic hypoglycaemic clamp, induced by insulin infusion (HypoG, n = 5); hyperinsulinemic euglycaemic clamp, induced by insulin and glucose infusion (EuG; n = 6); control, receiving a saline solution infusion (NaCl; n = 6). At 48 h after the start of i.v. infusions, two udder quarters per cow were challenged with 200 μg of E. coli lipopolysaccharide (LPS). Individual blood samples were taken before clamps, before LPS challenge (i.e. 48 h after clamps) and 6.5 h after. At 48 h, positive acute phase proteins (posAPP) did not differ among groups, whereas albumin and cholesterol (index of lipoproteins), negative APP (negAPP), were lower (p < 0.05) in EuG compared to NaCl and HypoG. The concentration of IL-6 was greater in EuG (p < 0.05) but only vs. HypoG. At 6.5 h following LPS challenge, IL-6 increased in the NaCl and EuG clamps (p < 0.05), while TNF-α increased (p < 0.05) in the EuG only. Among the posAPP, haptoglobin markedly increased in EuG (p < 0.05), but not in NaCl (p = 0.76) and in HypoG; ceruloplasmin tended to decline during LPS challenge, the reduction was significant when all animals were considered (p < 0.05). Conversely, all the negAPP showed a marked reduction 6.5 h after LPS challenge in the three groups. In conclusion, EuG caused an inflammatory status after 48-h infusion (i.e. decrease of negAPP) and induced a quicker acute phase response (e.g. marked rise of TNF-α, IL-6) after the intramammary LPS challenge. These data suggest that the simultaneous high availability of glucose and insulin at the tissue-level makes dairy cows more susceptible to inflammatory events. In contrast, HypoG seems to attenuate the inflammatory response. Journal of Animal Physiology and Animal Nutrition © 2016 Blackwell Verlag GmbH.

Temperature Behavior of Possible Cave Skylight on Mars

NASA Technical Reports Server (NTRS)

2007-01-01

[figure removed for brevity, see original site] Figure 1

Each of the three images in this set covers the same patch of Martian ground, centered on a possible cave skylight informally called 'Annie,' which has a diameter about double the length of a football field. The Thermal Emission Imaging System camera on NASA's Mars Odyssey orbiter took all three, gathering information that the hole is cooler than surrounding surface in the afternoon and warmer than the surrounding surface at night. This is thermal behavior that would be expected from an opening into an underground space.

The left image was taken in visible-wavelength light (figure 1). The other two were taken in thermal infrared wavelengths, indicating the relative temperatures of features in the image. The center image is from mid-afternoon. The hole is warmer than the shadows of nearby pits to the north and south, while cooler than sunlit surfaces. The thermal image at right was taken in the pre-dawn morning, about 4 a.m. local time. At that hour, the hole is warmer than all nearby surfaces.

Annie and six other features with similar thermal behavior are on the northern slope of a high Martian volcano named Arsia Mons, which is at 9 degrees south latitude, 239 degrees east longitude.

Mars Odyssey is managed by NASA's Jet Propulsion Laboratory, a division of the California Institute of Technology, Pasadena, for NASA's Science Mission Directorate, Washington. Lockheed Martin Space Systems, Denver, is the prime contractor for the project and built the spacecraft. The orbiter's Thermal Emission Imaging System was developed by Arizona State University, Tempe, in collaboration with Raytheon Santa Barbara Remote Sensing, Santa Barbara, Calif., and is operated by Arizona State University.

[Effectiveness of school-based interventions to prevent traffic accidents: an overview of secondary literature].

PubMed

Guerriero, Chiara; Verdina, Federico; Ferri, Chiara; Pizzaguerra, Martina; Silvestri, Silvia; Tober, Nastassja; Ristagno, Quenya; Costa, Alessandro; Cerasuolo, Michele; Faggiano, Fabrizio; Allara, Elias

2016-01-01

INTRODUZIONE: gli incidenti stradali sono l'ottava causa di morte al mondo e la prima tra i giovani di 15-29 anni. In Italia il Piano nazionale sicurezza stradale raccomanda l'educazione scolastica per la prevenzione degli incidenti stradali; ad oggi non esistono documenti che raccolgano evidenze di efficacia sugli interventi educativi stradali e le rapportino al contesto italiano. OBIETTIVI: riassumere e discutere ciò che è noto in letteratura riguardo agli interventi scolastici per la prevenzione degli incidenti stradali. METODI: sono state ricercate linee guida e revisioni sistematiche usando i seguenti criteri di inclusione: popolazione di età inferiore ai 25 anni di entrambi i sessi; interventi scolastici di educazione stradale; effetti su indicatori primari di esito come riduzione degli incidenti stradali, astinenza dalla guida sotto l'effetto di alcol e dall'accettare passaggi in macchina da guidatori che sono sotto l'effetto di alcol; effetti su indicatori secondari di esito come conoscenze e competenze sui comportamenti di guida sicura. RISULTATI: sono state identificate due revisioni sistematiche. L'educazione stradale nelle scuole non mostra evidenza di efficacia (rischio relativo 1,03; IC95% 0,98-1,08) nel ridurre gli incidenti. Programmi scolastici più specifici mostrano risultati solo in parte convincenti per l'adozione di comportamenti sicuri come l'astinenza dal guidare sotto l'effetto di alcol e dall'accettare passaggi in macchina da guidatori che sono sotto l'effetto di alcol. DISCUSSIONE: le revisioni incluse non hanno trovato programmi efficaci nella riduzione degli incidenti stradali o dei fattori che possano determinarli. Nell'attesa di studi più recenti, appare opportuno promuovere l'implementazione di interventi misti, scolastici e di comunità, che hanno mostrato maggiori prove di efficacia.

Version 2 of the IASI NH3 neural network retrieval algorithm: near-real-time and reanalysed datasets

NASA Astrophysics Data System (ADS)

Van Damme, Martin; Whitburn, Simon; Clarisse, Lieven; Clerbaux, Cathy; Hurtmans, Daniel; Coheur, Pierre-François

2017-12-01

Recently, Whitburn et al.(2016) presented a neural-network-based algorithm for retrieving atmospheric ammonia (NH3) columns from Infrared Atmospheric Sounding Interferometer (IASI) satellite observations. In the past year, several improvements have been introduced, and the resulting new baseline version, Artificial Neural Network for IASI (ANNI)-NH3-v2.1, is documented here. One of the main changes to the algorithm is that separate neural networks were trained for land and sea observations, resulting in a better training performance for both groups. By reducing and transforming the input parameter space, performance is now also better for observations associated with favourable sounding conditions (i.e. enhanced thermal contrasts). Other changes relate to the introduction of a bias correction over land and sea and the treatment of the satellite zenith angle. In addition to these algorithmic changes, new recommendations for post-filtering the data and for averaging data in time or space are formulated. We also introduce a second dataset (ANNI-NH3-v2.1R-I) which relies on ERA-Interim ECMWF meteorological input data, along with surface temperature retrieved from a dedicated network, rather than the operationally provided Eumetsat IASI Level 2 (L2) data used for the standard near-real-time version. The need for such a dataset emerged after a series of sharp discontinuities were identified in the NH3 time series, which could be traced back to incremental changes in the IASI L2 algorithms for temperature and clouds. The reanalysed dataset is coherent in time and can therefore be used to study trends. Furthermore, both datasets agree reasonably well in the mean on recent data, after the date when the IASI meteorological L2 version 6 became operational (30 September 2014).

Statistical Field Estimation and Scale Estimation for Complex Coastal Regions and Archipelagos

DTIC Science & Technology

2009-05-01

instruments applied to mode-73. Deep-Sea Research, 23:559–582. Brown , R. G. and Hwang , P. Y. C. (1997). Introduction to Random Signals and Applied Kalman ...the covariance matrix becomes neg- ative due to numerical issues ( Brown and Hwang , 1997). Some useful techniques to counter these divergence problems...equations ( Brown and Hwang , 1997). If the number of observations is large, divergence problems can arise under certain con- ditions due to truncation errors

Four-Channel Threshold Detector With Optical Isolation

DTIC Science & Technology

2009-02-01

1uF tantalum 35 V Capacitor 6 75ohm 1l4 W Carbon or Metal Film Resistor I .1 uF tantalum 35V Capacitor 37 HCC / HCF 40688 CMOS 8 input NAND / AND 1...100uF aluminum 35 V Capacitor 3 1M340T15 1 5 V, 1 A TO –220 Regulator 1 MC7915ACT neg15 V, 1 A TO –220 Regulator 1 35952503 50 Kohm, 10-turn

ELECTRON CLOUD OBSERVATIONS AND CURES IN RHIC

DOE Office of Scientific and Technical Information (OSTI.GOV)

FISCHER,W.; BLASKIEWICZ, M.; HUANG, H.

Since 2001 RHIC has experienced electron cloud effects, which have limited the beam intensity. These include dynamic pressure rises - including pressure instabilities, tune shifts, a reduction of the stability threshold for bunches crossing the transition energy, and possibly incoherent emittance growth. We summarize the main observations in operation and dedicated experiments, as well as countermeasures including baking, NEG coated warm beam pipes, solenoids, bunch patterns, anti-grazing rings, pre-pumped cold beam pipes, scrubbing, and operation with long bunches.

Energy requirements for growth of pubertal female Saanen goats.

PubMed

Figueiredo, F O M; Berchielli, T T; Resende, K T; Gomes, H F B; Almeida, A K; Sakomura, N K; Teixeira, I A M A

2016-04-01

Previous research on energy requirements of female Saanen goats, using the factorial approach, has not considered the specific requirements for maintenance and growth during the pubertal phase. Thus, the purpose of this study was to estimate energy requirements for maintenance (Trial 1) and growth (Trial 2) of non-pregnant and non-lactating female Saanen goats at the pubertal phase from 30 to 45 kg. In Trial 1, the net energy requirements for maintenance (NEm ) were estimated using 18 female Saanen goats randomly assigned to three levels of intake: ad libitum, and 70% and 40% of ad libitum intake. These animals were pair-fed in six slaughter groups, each consisting of one animal for each level of intake. In Trial 2, the net energy requirements for growth (NEg ) were estimated using 18 female Saanen goats, which were fed ad libitum and slaughtered at targeted BW of 30, 38 and 45 kg. The NEm was 52 kcal/kg(0.75) of BW. The NEg increased from 3.5 to 4.7 Mcal/kg of BW gain as BW increased from 30 to 45 kg. Our results suggest that the guidelines of the major feeding systems for the entire growth phase may not be adequate for females at pubertal phase. Journal of Animal Physiology and Animal Nutrition © 2015 Blackwell Verlag GmbH.

Langerin negative dendritic cells promote potent CD8+ T-cell priming by skin delivery of live adenovirus vaccine microneedle arrays

PubMed Central

Bachy, Veronique; Hervouet, Catherine; Becker, Pablo D.; Chorro, Laurent; Carlin, Leo M.; Herath, Shanthi; Papagatsias, Timos; Barbaroux, Jean-Baptiste; Oh, Sea-Jin; Benlahrech, Adel; Athanasopoulos, Takis; Dickson, George; Patterson, Steven; Kwon, Sung-Yun; Geissmann, Frederic; Klavinskis, Linda S.

2013-01-01

Stabilization of virus protein structure and nucleic acid integrity is challenging yet essential to preserve the transcriptional competence of live recombinant viral vaccine vectors in the absence of a cold chain. When coupled with needle-free skin delivery, such a platform would address an unmet need in global vaccine coverage against HIV and other global pathogens. Herein, we show that a simple dissolvable microneedle array (MA) delivery system preserves the immunogenicity of vaccines encoded by live recombinant human adenovirus type 5 (rAdHu5). Specifically, dried rAdHu5 MA immunization induced CD8+ T-cell expansion and multifunctional cytokine responses equipotent with conventional injectable routes of immunization. Intravital imaging demonstrated MA cargo distributed both in the epidermis and dermis, with acquisition by CD11c+ dendritic cells (DCs) in the dermis. The MA immunizing properties were attributable to CD11c+ MHCIIhi CD8αneg epithelial cell adhesion molecule (EpCAMneg) CD11b+ langerin (Lang; CD207)neg DCs, but neither Langerhans cells nor Lang+ DCs were required for CD8+ T-cell priming. This study demonstrates an important technical advance for viral vaccine vectors progressing to the clinic and provides insights into the mechanism of CD8+ T-cell priming by live rAdHu5 MAs. PMID:23386724

Conformational and mechanical changes of DNA upon transcription factor binding detected by a QCM and transmission line model.

PubMed

de-Carvalho, Jorge; Rodrigues, Rogério M M; Tomé, Brigitte; Henriques, Sílvia F; Mira, Nuno P; Sá-Correia, Isabel; Ferreira, Guilherme N M

2014-04-21

A novel quartz crystal microbalance (QCM) analytical method is developed based on the transmission line model (TLM) algorithm to analyze the binding of transcription factors (TFs) to immobilized DNA oligoduplexes. The method is used to characterize the mechanical properties of biological films through the estimation of the film dynamic shear moduli, G and G, and the film thickness. Using the Saccharomyces cerevisiae transcription factor Haa1 (Haa1DBD) as a biological model two sensors were prepared by immobilizing DNA oligoduplexes, one containing the Haa1 recognition element (HRE(wt)) and another with a random sequence (HRE(neg)) used as a negative control. The immobilization of DNA oligoduplexes was followed in real time and we show that DNA strands initially adsorb with low or non-tilting, laying flat close to the surface, which then lift-off the surface leading to final film tilting angles of 62.9° and 46.7° for HRE(wt) and HRE(neg), respectively. Furthermore we show that the binding of Haa1DBD to HRE(wt) leads to a more ordered and compact film, and forces a 31.7° bending of the immobilized HRE(wt) oligoduplex. This work demonstrates the suitability of the QCM to monitor the specific binding of TFs to immobilized DNA sequences and provides an analytical methodology to study protein-DNA biophysics and kinetics.

Persistence of ZIKV-RNA in the cellular fraction of semen is accompanied by a surrogate-marker of viral replication. Diagnostic implications for sexual transmission.

PubMed

Biava, Mirella; Caglioti, Claudia; Castilletti, Concetta; Bordi, Licia; Carletti, Fabrizio; Colavita, Francesca; Quartu, Serena; Nicastri, Emanuele; Iannetta, Marco; Vairo, Francesco; Liuzzi, Giuseppina; Taglietti, Fabrizio; Ippolito, Giuseppe; Capobianchi, Maria Rosaria; Lalle, Eleonora

2018-01-01

As asymptomatic infections represent 80% of ZIKV-infected individuals, sexual transmission is a rising concern. Recent studies highlighted a preferential association of ZIKV with the cellular fraction (CF) of different specimen types. Our aim was to evaluate the presence of ZIKV-RNA in different body fluids, focusing on semen specimens to assess the ZIKV-RNA content in either the unfractionated sample, its CF or seminal plasma (SP). In addition, to establish if the presence of ZIKV genome was associated with active virus replication, we measured the levels of negative-strand ZIKV-RNA. ZIKV total-RNA was detected in blood, urine and unfractionated semen, and neg-RNA in semen CF and SP samples longitudinally collected from two ZIKV-positive men followed at the National Institute for Infectious Diseases "L. Spallanzani", Italy. In both patients, ZIKV total-RNA was detected in CF with ct values always lower than in the corresponding unfractionated samples, and was observed even in the CF from negative unfractionated semen samples. In Patient 2, neg-RNA was also detected in CF, suggesting ongoing viral replication. Our results demonstrate higher clinical sensitivity of CF as compared to whole semen testing, emphasizing the need to extend ZIKV-RNA testing to CF, to rule out virus presence and the possible risk of sexual transmission.

T cell infiltration into Ewing sarcomas is associated with local expression of immune-inhibitory HLA-G.

PubMed

Spurny, Christian; Kailayangiri, Sareetha; Altvater, Bianca; Jamitzky, Silke; Hartmann, Wolfgang; Wardelmann, Eva; Ranft, Andreas; Dirksen, Uta; Amler, Susanne; Hardes, Jendrik; Fluegge, Maike; Meltzer, Jutta; Farwick, Nicole; Greune, Lea; Rossig, Claudia

2018-01-19

Ewing sarcoma (EwS) is an aggressive mesenchymal cancer of bones or soft tissues. The mechanisms by which this cancer interacts with the host immune system to induce tolerance are not well understood. We hypothesized that the non-classical, immune-inhibitory HLA-molecule HLA-G contributes to immune escape of EwS. While HLA-G pos suppressor T cells were not increased in the peripheral blood of EwS patients, HLA-G was locally expressed on the tumor cells and/or on infiltrating lymphocytes in 16 of 47 pretherapeutic tumor biopsies and in 4 of 12 relapse tumors. HLA-G expression was not associated with risk-related patient variables or response to standard chemotherapy, but with significantly increased numbers of tumor-infiltrating CD3+ T cells compared to HLA-G neg EwS biopsies. In a mouse model, EwS xenografts after adoptive therapy with tumor antigen-specific CAR T cells strongly expressed HLA-G whereas untreated control tumors were HLA-G neg . IFN-γ stimulation of EwS cell lines in vitro induced expression of HLA-G protein. We conclude that EwS cells respond to tumor-infiltrating T cells by upregulation of HLA-G, a candidate mediator of local immune escape. Strategies that modulate HLA-G expression in the tumor microenvironment may enhance the efficacy of cellular immunotherapeutics in this cancer.

Two-dimensional turbulence cross-correlation functions in the edge of NSTX

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zweben, S. J.; Stotler, D. P.; Scotti, F.

The 2D radial vs. poloidal cross-correlation functions of edge plasma turbulence were measured near the outer midplane using a gas puff imaging (GPI) diagnostic on NSTX. These correlation functions were evaluated at radii r = 0 cm, ±3 cm, and ±6 cm from the separatrix and poloidal locations p = 0 cm and ±7.5 cm from the GPI poloidal center line for 20 different shots. The ellipticity ε and tilt angle φ of the positive cross-correlation regions and the minimum negative cross-correlation “cmin” and total negative over positive values “neg/pos” were evaluated for each of these cases. The average resultsmore » over this dataset were ε = 2.2 ± 0.9, φ = 87° ± 34° (i.e., poloidally oriented), cmin =-0.30 ± 0.15, and neg/pos = 0.25 ± 0.24. Thus, there was a significant variation in these correlation results within this database, with dependences on the location within the image, the magnetic geometry, and the plasma parameters. In conclusion, possible causes for this variation are discussed, including the misalignment of the GPI view with the local B field line, the magnetic shear of field lines at the edge, the poloidal flow shear of the turbulence, blob-hole correlations, and the neutral density 'shadowing' effect in GPI.« less

Two-dimensional turbulence cross-correlation functions in the edge of NSTX

DOE PAGES

Zweben, S. J.; Stotler, D. P.; Scotti, F.; ...

2017-09-26

The 2D radial vs. poloidal cross-correlation functions of edge plasma turbulence were measured near the outer midplane using a gas puff imaging (GPI) diagnostic on NSTX. These correlation functions were evaluated at radii r = 0 cm, ±3 cm, and ±6 cm from the separatrix and poloidal locations p = 0 cm and ±7.5 cm from the GPI poloidal center line for 20 different shots. The ellipticity ε and tilt angle φ of the positive cross-correlation regions and the minimum negative cross-correlation “cmin” and total negative over positive values “neg/pos” were evaluated for each of these cases. The average resultsmore » over this dataset were ε = 2.2 ± 0.9, φ = 87° ± 34° (i.e., poloidally oriented), cmin =-0.30 ± 0.15, and neg/pos = 0.25 ± 0.24. Thus, there was a significant variation in these correlation results within this database, with dependences on the location within the image, the magnetic geometry, and the plasma parameters. In conclusion, possible causes for this variation are discussed, including the misalignment of the GPI view with the local B field line, the magnetic shear of field lines at the edge, the poloidal flow shear of the turbulence, blob-hole correlations, and the neutral density 'shadowing' effect in GPI.« less

Characterization of Dendritic Cells Subpopulations in Skin and Afferent Lymph in the Swine Model

PubMed Central

Marquet, Florian; Bonneau, Michel; Pascale, Florentina; Urien, Celine; Kang, Chantal; Schwartz-Cornil, Isabelle; Bertho, Nicolas

2011-01-01

Transcutaneous delivery of vaccines to specific skin dendritic cells (DC) subsets is foreseen as a promising strategy to induce strong and specific types of immune responses such as tolerance, cytotoxicity or humoral immunity. Because of striking histological similarities between human and pig skin, pig is recognized as the most suitable model to study the cutaneous delivery of medicine. Therefore improving the knowledge on swine skin DC subsets would be highly valuable to the skin vaccine field. In this study, we showed that pig skin DC comprise the classical epidermal langerhans cells (LC) and dermal DC (DDC) that could be divided in 3 subsets according to their phenotypes: (1) the CD163neg/CD172aneg, (2) the CD163highCD172apos and (3) the CD163lowCD172apos DDC. These subtypes have the capacity to migrate from skin to lymph node since we detected them in pseudo-afferent lymph. Extensive phenotyping with a set of markers suggested that the CD163high DDC resemble the antibody response-inducing human skin DC/macrophages whereas the CD163negCD172low DDC share properties with the CD8+ T cell response-inducing murine skin CD103pos DC. This work, by showing similarities between human, mouse and swine skin DC, establishes pig as a model of choice for the development of transcutaneous immunisation strategies targeting DC. PMID:21298011

An Atlas of Original and Mercator-Transformed Satellite-Data Images of the Alboran Sea, August-October 1983,

DTIC Science & Technology

1985-08-01

the movements of the thermal front south of Capo de Geta (northeastern limit of the Alboran Sea) both westwards and eastwards. This demonstrates how...tactical applications guide, operational environmental satellite; polar-orbiting satellites and geostationary satellites; spacecraft, sensors , imagery...DETERMINATE L’UMIDITA’ RELATIVA E LA TEMPERATURA DI RUGIADA 0 DI BRINA MEDIANTE LO PSICROMETRO. AERONAUTICA MILITARE Italiana. Ispettorato delle

Assessing the Adaptability to Irregular Rest-Work Rhythms in Military Personnel

DTIC Science & Technology

2000-03-01

Aeronautica Militare Italiana, CSV Reparto Medicina Aerospaziale Pratica di Mare, 00040 Pomezia (Roma) Italy 2 Dipartimento di Psicologia - Universitd...characteristics to adapt to increased work errors and impaired social work at unusual hours. and family relationship. In addition, there is an increased mortality...DUTY: a brief questionnaire on critical evaluation. Journal of Personality the sleep-wake cycle and vigilance filled in and Social Psychology. 58: 844

Asteroidi

NASA Astrophysics Data System (ADS)

Foglia, Sergio; Morbidelli, Alessandro; Masi, Gianluca; Carusi, Andrea

2006-10-01

Contents: Parte I: Asteroidi. 1. Introduzione. 2. Dinamica degli asteroidi. 3. Distribuzione degli asteroidi. 4. Proprietà fisiche degli asteroidi. 5. Interrelazioni nel Sistema Solare. Parte II: Le Osservazioni. 6. La pianificazione. 7. Astrometria. 8. Fotometria. Appendici: A. Tempo delle Effemeridi e Tempo Universale. B. Calcolo Effemeridi. C. Guida all'utilizzo del Minor Planet Software rel. 8. D. Determinazione della Coordinate Geografiche di una postazione di osservazione dalle Coordinate Metriche U.T.M. nella principale cartografia italiana.

List of Standards to Accompany Manual of Documentation Practices Applicable to Defence-Aerospace Scientific and Technical Information (Liste des Normes a Placer en Annexe au Manuel Concernant les Techniques Documentaires Applicables a l’Information Scientifique et Technique de la Defense et du Secteur Aerospatial)

DTIC Science & Technology

1990-10-01

CHARACTERS ISO 0233 1984 DOCUMENTATION - TRANSLITERATION OF ARABIC CHARACTERS INTO LATIN CHARACTERS ISO 0259 1954 DOCUMENTATION - TRANSLITERATION OF HEBREW...TRANSLITERATION OF ARABIC CHARACTERS IN LATIN CHARACTERS SF I 46-DUO 1N64 TRANSLITERATION - TRANSLITERATION OF HEBREW IN LATIN CHARACTERS . 46-010...LANGUAGE CODES (ANNIE: AUT.ORITY SYMSOLS DIN 31 634 CONVERSION OF THE GREEN ALUBABET DIN 31 635 CONVERSION OF THE ARABIC ALPHABET DIN 31 635 CONVERSION OF

Disability Awareness and Action Working Group (DAAWG) Event with

NASA Image and Video Library

2017-10-12

Dr. Temple Grandin, second from left, pauses for a photo after giving the keynote presentation at Kennedy Space Center's annual National Disability Employment Awareness Month (NDEAM) event. From left are Chief Financial Officer Susan Kroskey, executive champion of Kennedy's Disability Awareness and Action Working Group, or DAAWG; Grandin; Joette Feeney, chair of the Kennedy Networking Opportunities for Women group, or KNOW; and DAAWG Co-chairs Nicole Delvesco and Annie Williams. A prominent author and speaker on animal behavior and autism, Grandin is a professor of animal science at Colorado State University in Fort Collins, Colorado. Kennedy's DAAWG and KNOW groups partnered to sponsor the presentation.

[ELIE METCHNIKOFF--THE FOUNDER OF LONGEVITY SCIENCE AND A FOUNDER OF MODERN MEDICINE: IN HONOR OF THE 170TH ANNIVERSARY].

PubMed

Stambler, I S

2015-01-01

The years 2015-2016 mark a double anniversary--the 170th anniversary of birth and the 100th anni- versary of death--of one of the greatest Russian scientists, a person that may be considered a founding figure of modern immunology, aging and longevity science--Elie Metchnikoff (May 15, 1845-July 15, 1916). At this time of the rapid aging of the world population and the rapid development of technologies that may ameliorate degenerative aging processes, Metchnikoff's pioneering contribution to the search for anti-aging and healthspan-extending means needs to be recalled and honored.

Trasforiiazioni Termoelastiche Finite di Solidi Incomprimibili

NASA Astrophysics Data System (ADS)

Signorini, A.

Queste lezlioni hanno come direttiva una sintesi di quanto si trova sistematicamente sviluppato in una mia Memoria sulle trasformazioni termoelastiche finite di solidi incomprimibili, in corso di stampa negli Annali di Matematica pura e applicata t. XXXIX ( 1955) pp. 147-201 , Verranno anche esposti, come necessaria premessa, alcuni d ei risultati di due precedenti Memorie degli stessi Annali. Invece, per motivo di brevità, non potrò dare neppure un cenno delle ulteriori ricerche svilup pate dal prof. T. Manacorda in tre recentissimi suoi lavori:

Status of NSLS-II Storage Ring Vacuum Systems

DOE Office of Scientific and Technical Information (OSTI.GOV)

Doom,L.; Hseuh,H.; Ferreira, M.

2009-05-04

National Synchrotron Light Source II (NSLS-II), being constructed at Brookhaven National Laboratory, is a 3-GeV, high-flux and high- brightness synchrotron radiation facility with a nominal current of 500 mA. The storage ring vacuum system will have extruded aluminium chambers with ante-chamber for photon fans and distributed NEG strip pumping. Discrete photon absorbers will be used to intercept the un-used bending magnet radiation. In-situ bakeout will be implemented to achieve fast conditioning during initial commissioning and after interventions.

Exploring Evidence Aggregation Methods and External Expansion Sources for Medical Record Search

DTIC Science & Technology

2012-11-01

Equation 3 using Indri in the same way as our previous work [12]. We denoted this model as MRM . A Combined Model We linearly combine MRF and MRM to get...retrieving indexing visits ranking III RbM VRM baseline/MRF/ MRM models ICD, NEG MbR Figure 1: Merging results from two different...retrieval model MRM with one expansion collection at a time to explore the expansion effectiveness of each collection as show in Table 5. As we can

Individual Differences in Vigilance and Performance During Continuous/Sustained Operations

DTIC Science & Technology

2002-11-01

Casagrande Dipartimento di Psicologia UniversitA degli Studi di Roma "La Sapienza" Via dei Marsi, 78 -00185 Roma, Italy Aeronautica Militare Italiana...8217based. approach, Journal of Personality and Social Psychologv. 56, 267-283. Casagrande M, Violani C, Curcio G., Bertini M. (1997) Individual differences...Multidimensional assessment of coping: A critical evaluation. Journal of Personality and Social Psychology. 58: 844-854. Evans FJ, Cook MR, Cohen HD, Ome EC

Ricadute metalinguistiche dell'insegnamento dell'esperanto sulla lingua materna dell'alunno: Un'esperienza nella scuola media italiana (Metalinguistic Benefits of Planned Language Instruction: The Case of Esperanto in an Italian Secondary School).

ERIC Educational Resources Information Center

Pinto, Maria Antonietta; Corsetti, Renato

2001-01-01

Two groups of secondary school students participated in an experiment on the effect of Esperanto instruction on their metalinguistic abilities. The group that studied Esperanto for a full school year achieved better results on independently developed tests of metalinguistic ability in comparison with the control group. Results suggest the teaching…

The effect of positive and negative message framing on short term continuous positive airway pressure compliance in patients with obstructive sleep apnea

PubMed Central

Czaban, Marcin; Berry, Marc P.; Nirmalan, Prajeshan; Brown, Richard; Birdseye, Adam; Woroszyl, Asia; Chapman, Julia; Kent, Brian D.; Hart, Nicholas; Rossi, Gian Paolo; Steier, Joerg

2018-01-01

Background Continuous positive airway pressure (CPAP), the best available treatment for obstructive sleep apnea (OSA), requires long-term compliance to be effective. Behavioral interventions may be used to improve adherence to CPAP. We aimed to investigate whether positive or negative message framing impacts on CPAP compliance in patients with OSA, when compared to standard care. Methods Consenting patients with confirmed OSA were randomly allocated to receive along with their CPAP either positively or negatively framed messages (Pos; Neg), or standard care (Con). Standardized motivational messages were read out to patients during an initial teaching session and through weekly telephone calls. Patients’ compliance data were reviewed 2 and 6 weeks following CPAP initiation. Results We randomized 112 patients to groups that were matched for age, BMI, and OSA severity. The positively framed group (Pos) showed greater CPAP usage after 2 weeks (total use 53.7±31.4 hours) as compared to the negatively framed and the control group (35.6±27.4 and 40.8±33.5 hours, P<0.05); however, no differences were seen at 6 weeks. There were more dropouts in the control group than in either framed groups (Pos n=5; Neg n=8; Con n=11; P<0.05). Conclusions Positively framed messages can improve CPAP adherence in patients with OSA in the short-term; however, strategies for implementing its long-term use need to be developed. PMID:29445540

The effect of positive and negative message framing on short term continuous positive airway pressure compliance in patients with obstructive sleep apnea.

PubMed

Pengo, Martino F; Czaban, Marcin; Berry, Marc P; Nirmalan, Prajeshan; Brown, Richard; Birdseye, Adam; Woroszyl, Asia; Chapman, Julia; Kent, Brian D; Hart, Nicholas; Rossi, Gian Paolo; Steier, Joerg

2018-01-01

Continuous positive airway pressure (CPAP), the best available treatment for obstructive sleep apnea (OSA), requires long-term compliance to be effective. Behavioral interventions may be used to improve adherence to CPAP. We aimed to investigate whether positive or negative message framing impacts on CPAP compliance in patients with OSA, when compared to standard care. Consenting patients with confirmed OSA were randomly allocated to receive along with their CPAP either positively or negatively framed messages (Pos; Neg), or standard care (Con). Standardized motivational messages were read out to patients during an initial teaching session and through weekly telephone calls. Patients' compliance data were reviewed 2 and 6 weeks following CPAP initiation. We randomized 112 patients to groups that were matched for age, BMI, and OSA severity. The positively framed group (Pos) showed greater CPAP usage after 2 weeks (total use 53.7±31.4 hours) as compared to the negatively framed and the control group (35.6±27.4 and 40.8±33.5 hours, P<0.05); however, no differences were seen at 6 weeks. There were more dropouts in the control group than in either framed groups (Pos n=5; Neg n=8; Con n=11; P<0.05). Positively framed messages can improve CPAP adherence in patients with OSA in the short-term; however, strategies for implementing its long-term use need to be developed.

Increased burden of deleterious variants in essential genes in autism spectrum disorder.

PubMed

Ji, Xiao; Kember, Rachel L; Brown, Christopher D; Bućan, Maja

2016-12-27

Autism spectrum disorder (ASD) is a heterogeneous, highly heritable neurodevelopmental syndrome characterized by impaired social interaction, communication, and repetitive behavior. It is estimated that hundreds of genes contribute to ASD. We asked if genes with a strong effect on survival and fitness contribute to ASD risk. Human orthologs of genes with an essential role in pre- and postnatal development in the mouse [essential genes (EGs)] are enriched for disease genes and under strong purifying selection relative to human orthologs of mouse genes with a known nonlethal phenotype [nonessential genes (NEGs)]. This intolerance to deleterious mutations, commonly observed haploinsufficiency, and the importance of EGs in development suggest a possible cumulative effect of deleterious variants in EGs on complex neurodevelopmental disorders. With a comprehensive catalog of 3,915 mammalian EGs, we provide compelling evidence for a stronger contribution of EGs to ASD risk compared with NEGs. By examining the exonic de novo and inherited variants from 1,781 ASD quartet families, we show a significantly higher burden of damaging mutations in EGs in ASD probands compared with their non-ASD siblings. The analysis of EGs in the developing brain identified clusters of coexpressed EGs implicated in ASD. Finally, we suggest a high-priority list of 29 EGs with potential ASD risk as targets for future functional and behavioral studies. Overall, we show that large-scale studies of gene function in model organisms provide a powerful approach for prioritization of genes and pathogenic variants identified by sequencing studies of human disease.

Increased burden of deleterious variants in essential genes in autism spectrum disorder

PubMed Central

Kember, Rachel L.; Brown, Christopher D.; Bućan, Maja

2016-01-01

Autism spectrum disorder (ASD) is a heterogeneous, highly heritable neurodevelopmental syndrome characterized by impaired social interaction, communication, and repetitive behavior. It is estimated that hundreds of genes contribute to ASD. We asked if genes with a strong effect on survival and fitness contribute to ASD risk. Human orthologs of genes with an essential role in pre- and postnatal development in the mouse [essential genes (EGs)] are enriched for disease genes and under strong purifying selection relative to human orthologs of mouse genes with a known nonlethal phenotype [nonessential genes (NEGs)]. This intolerance to deleterious mutations, commonly observed haploinsufficiency, and the importance of EGs in development suggest a possible cumulative effect of deleterious variants in EGs on complex neurodevelopmental disorders. With a comprehensive catalog of 3,915 mammalian EGs, we provide compelling evidence for a stronger contribution of EGs to ASD risk compared with NEGs. By examining the exonic de novo and inherited variants from 1,781 ASD quartet families, we show a significantly higher burden of damaging mutations in EGs in ASD probands compared with their non-ASD siblings. The analysis of EGs in the developing brain identified clusters of coexpressed EGs implicated in ASD. Finally, we suggest a high-priority list of 29 EGs with potential ASD risk as targets for future functional and behavioral studies. Overall, we show that large-scale studies of gene function in model organisms provide a powerful approach for prioritization of genes and pathogenic variants identified by sequencing studies of human disease. PMID:27956632

Aggregation and self assembly of non-enzymatic glycation of collagen in the presence of amino guanidine and aspirin: an in vitro study.

PubMed

Usha, R; Jaimohan, S M; Rajaram, A; Mandal, A B

2010-10-01

Non-enzymatic glycation of collagen has been used in modern biomaterials science. This paper deals with in vitro studies on the effects of amino guanidine (AG) and aspirin in the non-enzymatic glycation (NEG) of collagen using thermal, conformational, fluorescence, turbidity and powder XRD measurements. There is no significant change in the fluorescence emission spectra for different concentrations of AG treated NEG of collagen whereas the emission intensity decreases as the concentration of aspirin increases. Circular dichroism (CD) revealed the disappearance of the positive peak at 220nm for glycated collagen in the presence of amino guanidine and aspirin suggesting the collapse of triple helical configuration. Nearly 15 degrees C decrease is observed in shrinkage temperature of glycated rat tail tendon (RTT) collagen fibres in the presence of aspirin. Powder XRD of glycated collagen nano-fibrils in the presence of amino guanidine reveals high crystalline nature and the enhancement of self assembly processes when compared to aspirin. To the best of our knowledge, this is the first report of powder XRD of the self assembly of collagen nano-fibrils without mineralization. Our experimental results suggest that in the non-enzymatic glycation of collagen both AG and aspirin play a pivotal role in the aggregation and self assembly processes. From the present study, it is possible to conclude that while AG significantly influences the self assembly processes, aspirin facilitates the aggregation processes.

Prognostic roles of absolute monocyte and absolute lymphocyte counts in patients with advanced-stage follicular lymphoma in the rituximab era: an analysis from the FOLL05 trial of the Fondazione Italiana Linfomi.

PubMed

Marcheselli, Luigi; Bari, Alessia; Anastasia, Antonella; Botto, Barbara; Puccini, Benedetta; Dondi, Alessandra; Carella, Angelo M; Alvarez, Isabel; Chiarenza, Annalisa; Arcari, Annalisa; Salvi, Flavia; Federico, Massimo

2015-05-01

Recently, in an attempt to improve the discrimination power of the international prognostic index (IPI), patients with diffuse large B-cell lymphoma were evaluated to determine the prognostic roles of peripheral blood absolute monocyte count (AMC) and absolute lymphocyte count (ALC). Here, we analysed data of 428 patients with follicular lymphoma (FL) enrolled in a prospective, randomized trial (FOLL05 study) conducted by Fondazione Italiana Linfomi, to assess the impact of AMC and ALC on progression-free survival (PFS). All patients had been treated with one of three treatment combinations: (i) rituximab (R) plus cyclophosphamide, vincristine and prednisone; (ii) R plus cyclophosphamide, doxorubicin, vincristine and prednisone or (iii) R plus mitoxantrone and fludarabine. We showed that only AMC was a powerful predictor of PFS, and possibly overall survival, in patients with FL treated with combination chemotherapy regimens that contained R. The AMC can be used alone as a novel, simple factor that can predict survival outcome in patients with FL, independent of the immunochemotherapy regimen. It may therefore be widely used by clinicians, due to its simplicity and broad applicability. Additionally, it can be combined with other factors that determine the IPI or FLIPI, to increase the discriminating ability of these indices. © 2015 John Wiley & Sons Ltd.

The diagnostic value of cytohistological urine analysis and cytokeratin 20 in malignant and atypical urothelial cells.

PubMed

Negri, S; Biavati, P; Bondi, A

2016-09-01

To determine the ability of cytohistology and cytokeratin 20 (CK 20) expression in malignant and atypical cells (AUC) from urine to serve as a diagnostic tool for assessing urothelial carcinoma (UC). Diagnoses from 55 urine cytological samples from 55 patients were analyzed and correlated with subsequent biopsy findings. A total of 50 archived urine slides from patients that received a cytological diagnosis and histological follow-up were selected for immunostaining with monoclonal CK 20 antibodies and elaborated by Z-test for proportions. The majority of all positive or atypical smears (24; 89%) were confirmed through histological analysis. The majority of urinary cytological diagnoses reported as negative (15; 54%) were also confirmed through biopsies. The overall sensitivity, specificity, PPV, and NPV were 65%, 83%, 89%, and 54%, respectively. All 13 smears cytologically determined to contain malignant cells, with subsequent biopsies confirming UC, exhibited strong positive staining with the CK 20 antibody. All cases evaluated as benign both cytologically and histologically had negative CK 20 staining. Of the 15 AUC cases with lesions confirmed through biopsies, 11 (73%) had atypical cells that stained positive for CK 20. Our results demonstrate the diagnostic value of urinary cytology and confirm CK 20 as an adjunct marker for the diagnosis of UC and for the triage of AUC. © Copyright Società Italiana di Anatomia Patologica e Citopatologia Diagnostica, Divisione Italiana della International Academy of Pathology.

Identification of Biomarkers for Footpad Dermatitis Development and Wound Healing

PubMed Central

Chen, Juxing; Tellez, Guillermo; Escobar, Jeffery

2016-01-01

Footpad dermatitis (FPD) is a type of skin inflammation that causes necrotic lesions on the plantar surface of the footpads in commercial poultry, with significant animal welfare, and economic implications. To identify biomarkers for FPD development and wound healing, a battery cage trial was conducted in which a paper sheet was put on the bottom of cages to hold feces to induce FPD of broilers. Day-of-hatch Ross 308 male broiler chicks were fed a corn–soybean meal diet and assigned to 3 treatments with 8 cages per treatment and 11 birds per cage. Cages without paper sheets were used as a negative control (NEG). Cages with paper sheets during the entire growth period (d 0–30) were used as a positive control (POS) to continually induce FPD. Cages with paper sheets during d 0–13 and without paper sheets during d 14–30 were used to examine the dynamic of FPD development and lesion wound healing (LWH). Footpad lesions were scored to grade (G) 1–5 with no lesion in G1 and most severe lesion in G5. Covering with paper sheets in POS and LWH induced 99% incidence of G3 footpads on d 13. Removing paper sheets from LWH healed footpad lesions by d 30. One representative bird, with lesions most close to pen average lesion score, was chosen to collect footpad skin samples for biomarker analysis. Total collagen protein and mRNA levels of tenascin X (TNX), type I α1 collagen (COL1A1), type III α1 collagen (COL3A1), tissue inhibitor of metalloproteinase 3 (TIMP3), and integrin α1 (ITGA1) mRNA levels were decreased (P < 0.05), while mRNA levels of tenascin C (TNC), tumor necrosis factor (TNF) α, Toll-like receptor (TLR) 4 and vascular endothelial growth factor (VEGF), IL-1β, and the ratio of MMP2 to all TIMP were increased (P < 0.03) in G3 footpads in POS and LWH compared to G1 footpads in NEG on d 14. These parameters continued to worsen with development of more severe lesions in POS. After paper sheets were removed (i.e., LWH), levels of these parameters gradually or rapidly returned to levels measured in NEG. Regression analysis indicated significant quadratic changes of these parameters to footpad lesion scores. In summary, these biomarkers were interrelated with dynamic changes of footpad lesion scores, suggesting they may be used as potential biomarkers for footpad lesion development and wound healing process. PMID:26973819

Depopulation of Caged Layer Hens with a Compressed Air Foam System

PubMed Central

Gurung, Shailesh; Hoffman, John; Stringfellow, Kendre; Abi-Ghanem, Daad; Zhao, Dan; Caldwell, David; Lee, Jason; Styles, Darrel; Berghman, Luc; Byrd, James; Farnell, Yuhua; Archer, Gregory

2018-01-01

Simple Summary Reportable diseases, such as avian influenza, spread rapidly among poultry, resulting in the death of a large number of birds. Once such a disease has been diagnosed at a farm, infected and susceptible birds are rapidly killed to prevent the spread of the disease. The methods to eliminate infected caged laying hens are limited. An experiment was conducted to study the effectiveness of foam made from compressed air, water, and soap to kill laying hens in cages. The study found that stress levels of the hens killed using compressed air foam in cages to be similar to the hens killed by carbon dioxide or the negative control. Hens exposed to carbon dioxide died earlier as compared to the foam methods. The authors conclude that application of compressed air foam in cages is an alternative to methods such as gas inhalation and ventilation shutdown to rapidly and humanely kill laying hens during epidemics. Abstract During the 2014–2015 US highly pathogenic avian influenza (HPAI) outbreak, 50.4 million commercial layers and turkeys were affected, resulting in economic losses of $3.3 billion. Rapid depopulation of infected poultry is vital to contain and eradicate reportable diseases like HPAI. The hypothesis of the experiment was that a compressed air foam (CAF) system may be used as an alternative to carbon dioxide (CO2) inhalation for depopulating caged layer hens. The objective of this study was to evaluate corticosterone (CORT) and time to cessation of movement (COM) of hens subjected to CAF, CO2 inhalation, and negative control (NEG) treatments. In Experiment 1, two independent trials were conducted using young and spent hens. Experiment 1 consisted of five treatments: NEG, CO2 added to a chamber, a CO2 pre-charged chamber, CAF in cages, and CAF in a chamber. In Experiment 2, only spent hens were randomly assigned to three treatments: CAF in cages, CO2 added to a chamber, and aspirated foam. Serum CORT levels of young hens were not significantly different among the CAF in cages, CAF in a chamber, NEG control, and CO2 inhalation treatments. However, spent hens subjected to the CAF in a chamber had significantly higher CORT levels than birds in the rest of the treatments. Times to COM of spent hens subjected to CAF in cages and aspirated foam were significantly greater than of birds exposed to the CO2 in a chamber treatment. These data suggest that applying CAF in cages is a viable alternative for layer hen depopulation during a reportable disease outbreak. PMID:29324639

Exploration of Biological Markers of Feed Efficiency in Young Bulls.

PubMed

Meale, Sarah J; Morgavi, Diego P; Cassar-Malek, Isabelle; Andueza, Donato; Ortigues-Marty, Isabelle; Robins, Richard J; Schiphorst, Anne-Marie; Laverroux, Sophie; Graulet, Benoit; Boudra, Hamid; Cantalapiedra-Hijar, Gonzalo

2017-11-15

The efficiency with which ruminants convert feed to desirable products is difficult to measure under normal commercial settings. We explored the use of potential biological markers from easily obtainable samples, that is, blood, hair, and feces, to characterize potential causes of divergent efficiency when considered as residual feed intake (RFI) or feed conversion efficiency (FCE). A total of 54 Charolais bulls, 20 in period 1 and 34 in period 2, were examined for individual dry matter intake (DMI) and growth. Bulls were offered a diet of 70:30 wrapped grass silage to concentrate for 99 d. At the conclusion of the test period, blood samples were collected for the determination of vitamins B 2 and B 6 , and plasma used for the determination of metabolites, natural isotopic 15 N abundance ( 15 N NIA, expressed as δ 15 N ‰) and fractionation (Δ 15 N plasma proteins-diet and Δ 13 C plasma proteins-diet ) and near-infrared spectroscopy (NIRS). Feces were analyzed by NIRS. Bulls were slaughtered at 15-17 months of age and carcass characteristics determined. Bulls were ranked according to RFI with extremes (SD ± 0.5; n = 31) classified as either efficient (Neg-RFI) or inefficient (Pos-RFI). Extreme bulls were then classified for FCE (high vs low FCE), changing the groups. Pos-RFI bulls consumed 14% more feed than Neg-RFI bulls for the same level of weight gain. Low FCE bulls tended to eat more, but had lower weight gains than high FCE bulls. No differences were detected in carcass conformation, fat scores, hot carcass weight, or dressing percentage. Yet, heart and bladder weights were heavier in Pos-RFI, and rumen weight tended to be heavier in Pos-RFI bulls. RFI did not affect bulk 15 N or 13 C fractionation. A negative correlation was observed between FCE and Δ 15 N plasma proteins-diet . Inefficient bulls (Pos-RFI) had higher δ 15 N in glycine compared to Neg-RFI bulls. Similarly, metabolomic analysis showed a tendency for concentrations of glycine and sarcosine to be elevated in Pos-RFI bulls, whereas aspartic acid and carnosine tended to be elevated, and serine tended to be lower in High FCE. Among vitamins, only flavin adenine dinucleotide concentration was higher in the blood of bulls with High FCE. These results suggest that the two feed efficiency metrics differ in the underlying mechanisms of metabolism, where RFI is driven by differences in the energetic requirements of visceral organs and the extent of AA catabolism.

On the Disposition of Maunders' Origninal Butterfly Diagram

NASA Astrophysics Data System (ADS)

Bogdan, T. J.

2000-05-01

On 21 May 1940, Annie S. D. Maunder mailed the original drawing of the celebrated ``Maunder Butterfly Diagram" to Stephen A., and his daughter Margaret L., Ionides. Later that same year Stephen and Margaret gave the diagram ``on indefinite loan" to Walter Orr Roberts, then the Superintendent of Fremont Pass Station of the Harvard College Observatory. The framed diagram remains on display today at the scion of that organization, the High Altitude Observatory of the National Center for Atmospheric Research, in Boulder Colorado. Drawing upon the original correspondences, this contribution recounts the story behind the travels of the ``Maunder Butterfly" during the second World War. The National Center for Atmospheric Research is sponsored by the National Science Foundation.

Annual Research Progress Report, Fiscal Year 1982,

DTIC Science & Technology

1982-10-01

1 ’k. Identification of Pathogenic W’ ’o Ss Ps nyt tnn jkw IL ) NIITM S,,sto,, SubiItttjd t . q1 in m c a i...8217,)pii n ’r’eti n in thp ’(’- il " 1 ,-. . .’’’’: Vr’’,,,i tT I tI the r,i Anni a 1 Mi I i tary - , ’ ’ 1 , _ . r t ’ 1 I , 1 , ’, 1 + .t , a I’ r" a tm I...Nwhorn Irtants. Amer Acad l-tr ils , nlstr ict VIIi, 7th A"nual Conference en Perinatal- ’e .iH i d i ci ( n, , I

The use of ERTS/LANDSAT imagery in relation to airborne remote sensing for terrain analysis in western Queensland, Australia

NASA Technical Reports Server (NTRS)

Cole, M. M.; Wen-Jones, S. (Principal Investigator)

1976-01-01

The author has identified the following significant results. Series of linears were identified on the March imagery of Lady Annie-Mt. Gordon fault zone area. The series with a WSW-ENE orientation which is normal to the major structural units and also several linears with NNW-SSE orientation appears to be particularly important. Copper mineralization is known at several localities where these linears are intersected by faults. Automated outputs using supervised methods involving the selection of training sets selected by visual recognition of spectral signatures on the color composites obtained from combinations of MSS bands 4, 5 and 7 projected through appropriate filters, were generated.

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Innovative T Cell-Targeted Therapy for Ovarian Cancer

DTIC Science & Technology

2012-10-01

from co-culture with EL4 -ROR1neg and EL4 -ROR1+ tumor targets. Ovarian cancer cell lines (A2780, EFO21, EFO27, IGROV1, OC314, and UPN251) were...profiled for ROR1 expression in normoxia (20% O2) and hypoxia (1% O2). Four-hour CRA was used to evaluate cytotoxicity against the OvCa and EL4 tumor...loaded aAPC for negative controls. EL4 is a murine T cell lymphoma cell line used to test specificity of CAR+ T cells with limited allo-reactivity

Management of diabetes at summer camps.

PubMed

Ciambra, Roberta; Locatelli, Chiara; Suprani, Tosca; Pocecco, Mauro

2005-01-01

We report our experience in the organization of diabetic children summer-camps since 1973. Guidelines for organization have been recently reported by the SIEDP (Società Italiana di Endocrinologia e Diabetologia Pediatrica). Our attention is focused on diabetes management at camp, organization and planning, medical staff composition and staff training, treatment of diabetes-related emergencies, written camp management plan, diabetes education and psychological issues at camp, prevention of possible risks, assessment of effectiveness of education in summer camps and research at camp.

The Dispersion Tensor and Its Unique Minimizer in Hashin-Shtrikman Micro-structures

NASA Astrophysics Data System (ADS)

Bălilescu, Loredana; Conca, Carlos; Ghosh, Tuhin; San Martín, Jorge; Vanninathan, Muthusamy

2018-05-01

In this paper, we introduce a macroscopic quantity, namely the dispersion tensor or the Burnett coefficients in the class of generalized Hashin-Shtrikman micro-structures (Tartar in The general theory of homogenization, volume 7 of Lecture notes of the Unione Matematica Italiana, Springer, Berlin, p 281, 2009). In the case of two-phase materials associated with the periodic Hashin-Shtrikman structures, we settle the issue that the dispersion tensor has a unique minimizer, which is the so called Apollonian-Hashin-Shtrikman micro-structure.

Meat Production in a Feedlot System of Zebu—Holstein Steers and Heifers with Dairy Genetics: Productive and Biological Analyses

PubMed Central

Menezes, Gustavo Chamon de Castro; Valadares Filho, Sebastião de Campos; Ruas, José Reinaldo Mendes; Detmann, Edenio; Menezes, Arismar de Castro; Zanett, Diego; Mariz, Lays Débora Silva; Rennó, Luciana Navajas; da Silva Junior, Jarbas Miguel

2014-01-01

The aim of this study was to evaluate the productive and biological efficiency of steers and heifers from dairy genetics in a feedlot system in terms of meat production. Twenty-four steers and 24 heifers at 10 monthes of age, (3/4) Zebu × (1/4) Holstein were utilized. They were distributed over four feedlot times, 30, 60, 90, and 120 days with four replications for each sex, and were slaughtered at the end of each period. The productive and biological analyses were performed through comparative slaughter to determine the body composition. Heifers presented with greater intakes (P < 0.05) of dry matter in grams per kg of body weight. Steers presented with a greater (P < 0.05) final empty body weight, carcass gain, cold carcass weight, and meat proportion in the carcass; however, heifers presented with a greater subcutaneous fat thickness (P < 0.05) and, consequently, a greater (P < 0.05) fat proportion in the carcass. We conclude that steers are more efficient in their productive performance than heifers in a feedlot. For the finishing carcass fat cover, heifers need 90 days in the feedlot. The net energy requirements for maintenance are 67 kcal/EBW0.75/d, and the net requirements of energy (NEg) and protein (NPg) for gain can be estimated by the following equations: NEg(Mcal/d) = 0.067 × EBW0.75 × EBG1.095 and NPg = 162 × EBG − 5.62 × RE for the two sexes. PMID:25574483

Adoptive cell therapy for lymphoma with CD4 T cells depleted of CD137-expressing regulatory T cells.

PubMed

Goldstein, Matthew J; Kohrt, Holbrook E; Houot, Roch; Varghese, Bindu; Lin, Jack T; Swanson, Erica; Levy, Ronald

2012-03-01

Adoptive immunotherapy with antitumor T cells is a promising novel approach for the treatment of cancer. However, T-cell therapy may be limited by the cotransfer of regulatory T cells (T(reg)). Here, we explored this hypothesis by using 2 cell surface markers, CD44 and CD137, to isolate antitumor CD4 T cells while excluding T(regs). In a murine model of B-cell lymphoma, only CD137(neg)CD44(hi) CD4 T cells infiltrated tumor sites and provided protection. Conversely, the population of CD137(pos)CD44hi CD4 T cells consisted primarily of activated T(regs). Notably, this CD137(pos) T(reg) population persisted following adoptive transfer and maintained expression of FoxP3 as well as CD137. Moreover, in vitro these CD137(pos) cells suppressed the proliferation of effector cells in a contact-dependent manner, and in vivo adding the CD137(pos)CD44(hi) CD4 cells to CD137(neg)CD44(hi) CD4 cells suppressed the antitumor immune response. Thus, CD137 expression on CD4 T cells defined a population of activated T(regs) that greatly limited antitumor immune responses. Consistent with observations in the murine model, human lymphoma biopsies also contained a population of CD137(pos) CD4 T cells that were predominantly CD25(pos)FoxP3(pos) T(regs). In conclusion, our findings identify 2 surface markers that can be used to facilitate the enrichment of antitumor CD4 T cells while depleting an inhibitory T(reg) population.

Chronic psoriatic skin inflammation leads to increased monocyte adhesion and aggregation

PubMed Central

Golden, Jackelyn B.; Groft, Sarah G.; Squeri, Michael V.; Debanne, Sara M.; Ward, Nicole L.; McCormick, Thomas S.; Cooper, Kevin D.

2015-01-01

Psoriasis patients exhibit an increased risk of death by cardiovascular disease (CVD) and have elevated levels of circulating intermediate (CD14++CD16+) monocytes. This elevation could represent evidence of monocyte dysfunction in psoriasis patients at risk of CVD, as increases in circulating CD14++CD16+ monocytes are predictive of myocardial infarction and death. An elevation in the CD14++CD16+ cell population has been previously reported in patients with psoriatic disease, which has been confirmed in the cohort of our human psoriasis patients. CD16 expression was induced in CD14++CD16neg classical monocytes following plastic adhesion, which also elicited enhanced β2 but not β1 integrin surface expression, suggesting increased adhesive capacity. Indeed, we found that psoriasis patients have increased monocyte aggregation among circulating PBMCs which is recapitulated in the KC-Tie2 murine model of psoriasis. Visualization of human monocyte aggregates using imaging cytometry revealed that classical CD14++CD16neg monocytes are the predominant cell type participating in these aggregate pairs. Many of these pairs also included CD16+ monocytes, which could account for apparent elevations of intermediate monocytes. Additionally, intermediate monocytes and monocyte aggregates were the predominant cell type to adhere to TNF-α and IL-17A-stimulated dermal endothelium. Ingenuity Pathway Analysis (IPA) demonstrated that monocyte aggregates have a distinct transcriptional profile from singlet monocytes and monocytes following plastic adhesion, suggesting that circulating monocyte responses to aggregation are not fully accounted for by homotypic adhesion, and that further factors influence their functionality. PMID:26223654

A CD22-reactive TCR from the T-cell allorepertoire for the treatment of acute lymphoblastic leukemia by TCR gene transfer

PubMed Central

Jahn, Lorenz; Hagedoorn, Renate S.; van der Steen, Dirk M.; Hombrink, Pleun; Kester, Michel G.D.; Schoonakker, Marjolein P.; de Ridder, Daniëlle; van Veelen, Peter A.; Falkenburg, J.H. Frederik; Heemskerk, Mirjam H.M.

2016-01-01

CD22 is currently evaluated as a target-antigen for the treatment of B-cell malignancies using chimeric antigen receptor (CAR)-engineered T-cells or monoclonal antibodies (mAbs). CAR- and mAbs-based immunotherapies have been successfully applied targeting other antigens, however, occurrence of refractory disease to these interventions urges the identification of additional strategies. Here, we identified a TCR recognizing the CD22-derived peptide RPFPPHIQL (CD22RPF) presented in human leukocyte antigen (HLA)-B*07:02. To overcome tolerance to self-antigens such as CD22, we exploited the immunogenicity of allogeneic HLA. CD22RPF-specific T-cell clone 9D4 was isolated from a healthy HLA-B*07:02neg individual, efficiently produced cytokines upon stimulation with primary acute lymphoblastic leukemia and healthy B-cells, but did not react towards healthy hematopoietic and nonhematopoietic cell subsets, including dendritic cells (DCs) and macrophages expressing low levels of CD22. Gene transfer of TCR-9D4 installed potent CD22-specificity onto recipient CD8+ T-cells that recognized and lysed primary B-cell leukemia. TCR-transduced T-cells spared healthy CD22neg hematopoietic cell subsets but weakly lysed CD22low-expressing DCs and macrophages. CD22-specific TCR-engineered T-cells could form an additional immunotherapeutic strategy with a complementary role to CAR- and antibody-based interventions in the treatment of B-cell malignancies. However, CD22 expression on non-B-cells may limit the attractiveness of CD22 as target-antigen in cellular immunotherapy. PMID:27689397

A CD22-reactive TCR from the T-cell allorepertoire for the treatment of acute lymphoblastic leukemia by TCR gene transfer.

PubMed

Jahn, Lorenz; Hagedoorn, Renate S; van der Steen, Dirk M; Hombrink, Pleun; Kester, Michel G D; Schoonakker, Marjolein P; de Ridder, Daniëlle; van Veelen, Peter A; Falkenburg, J H Frederik; Heemskerk, Mirjam H M

2016-11-01

CD22 is currently evaluated as a target-antigen for the treatment of B-cell malignancies using chimeric antigen receptor (CAR)-engineered T-cells or monoclonal antibodies (mAbs). CAR- and mAbs-based immunotherapies have been successfully applied targeting other antigens, however, occurrence of refractory disease to these interventions urges the identification of additional strategies. Here, we identified a TCR recognizing the CD22-derived peptide RPFPPHIQL (CD22RPF) presented in human leukocyte antigen (HLA)-B*07:02. To overcome tolerance to self-antigens such as CD22, we exploited the immunogenicity of allogeneic HLA. CD22RPF-specific T-cell clone 9D4 was isolated from a healthy HLA-B*07:02neg individual, efficiently produced cytokines upon stimulation with primary acute lymphoblastic leukemia and healthy B-cells, but did not react towards healthy hematopoietic and nonhematopoietic cell subsets, including dendritic cells (DCs) and macrophages expressing low levels of CD22. Gene transfer of TCR-9D4 installed potent CD22-specificity onto recipient CD8+ T-cells that recognized and lysed primary B-cell leukemia. TCR-transduced T-cells spared healthy CD22neg hematopoietic cell subsets but weakly lysed CD22low-expressing DCs and macrophages. CD22-specific TCR-engineered T-cells could form an additional immunotherapeutic strategy with a complementary role to CAR- and antibody-based interventions in the treatment of B-cell malignancies. However, CD22 expression on non-B-cells may limit the attractiveness of CD22 as target-antigen in cellular immunotherapy.

Post-transcriptional regulation of inducible nitric oxide synthase in chronic lymphocytic leukemia B cells in pro- and antiapoptotic culture conditions.

PubMed

Tiscornia, A C; Cayota, A; Landoni, A I; Brito, C; Oppezzo, P; Vuillier, F; Robello, C; Dighiero, G; Gabús, R; Pritsch, O

2004-01-01

Functional inducible NOS (iNOS) may be involved in the prolonged lifespan of chronic lymphocytic leukemia cells (B-CLL), although the exact mechanisms implicated remain elusive as yet. In this work, we have examined iNOS expression in normal B lymphocytes and B-CLL cells in pro- and antiapoptotic conditions. Our results demonstrate: (1) The existence of a new splice variant characterized by a complete deletion of exon 14 (iNOS 13-16(14del)), which was preferentially detected in normal B lymphocytes and may represent an isoform that could play a role in the regulation of enzyme activity. (2) The existence of another alternatively spliced iNOS mRNA transcript involving a partial deletion of the flavodoxin region (iNOS 13-16(neg)) was correlated to a decreased B-CLL cell viability. The 9-beta-D-arabinofuranosyl-2-fluoradenine or fludarabine (F-ara) treatment induced iNOS 13-16(neg) transcript variants, whereas IL-4 enhanced both the transcription of variants, including these exons (iNOS 13-16(pos)), and the expression of a 122 kDa iNOS protein. These results suggest that in B-CLL, a regulation process involving nitric oxide (.- NO) levels could occur by a post-transcriptional mechanism mediated by soluble factors. Our results also provide an insight into a new complementary proapoptotic action of F-ara in B-CLL by the induction of particular iNOS splice variants, leading to the activation of a caspase-3-dependent apoptotic pathway.

Effects of Combined Phase III and Phase II Cardiac Exercise Therapy for Middle-aged Male Patients with Acute Myocardial Infarction

PubMed Central

Lee, Chih-Wei; Wang, Ji-Hung; Hsieh, Jen-Che; Hsieh, Tsung-Cheng; Huang, Chien-Hui

2013-01-01

[Purpose] To investigate the effects of cardiac exercise therapy (CET) on exercise capacity and coronary risk factors (CRFs) of patients with acute myocardial infarction (AMI). [Methods] Patients who participated in an 8-week supervised, hospital-based phase II and 6-month home-based phase III CET with monthly telephone and/or home visits were defined as the exercise group (EG) (n=20), while those who did not receive phase II or phase III CET were defined as the no-exercise group (NEG) (n=10). CRFs were evaluated pre- and post-phase II and eight months after discharge. One and two-way repeated measures ANOVA were used to perform intra- and inter-group comparisons. [Results] Thirty men with AMI aged 49.3 ± 8.3 years were studied. EG increased their exercise capacity (METs) (6.8 ± 1.6 vs.10.0 ± 1.9) after phase II CET and was able to maintain it at 8-month follow-up. Both groups had significantly fewer persons who kept on smoking compared to the first examination. High density lipoprotein cholesterol (HDL-C) increased from 38.1 ± 11.0 to 43.7 ± 8.7 mg/dl at follow-up in EG while no significant difference was noted in NEG. [Conclusion] After phase III CET subjects had maintained the therapeutic effects of smoking cessation, and increasing exercise capacity obtained in phase II CET. HDL-C in EG continued to improve during phase III CET. PMID:24396201

How changes in nutrition have influenced the development of allergic diseases in childhood

PubMed Central

2012-01-01

The increasing prevalence of allergic diseases in childhood in the last decades could be linked to concomitant dietary changes, especially with the modified and lower consumption of fruit, vegetables and minerals. The consumption of these foods by pregnant women and children in the first years of life seems to be associated with a reduced risk of asthma and related symptoms. Foods that can prevent the development of wheezing through their antioxidant effects contain vitamin C and selenium; blood levels of these elements correlate negatively with the risk of wheezing. Intake of vitamin E during pregnancy also appears to be correlated with a reduced risk of wheezing for the unborn child. Similarly, low intake of zinc and carotenoids by pregnant women is associated with an increased risk of wheezing and asthma in childhood. Fiber also has anti-inflammatory properties and protective effects against allergic diseases such as atopic dermatitis and asthma. The consumption of fat influences the development of the airways. Populations in Western countries have increased their consumption of n-6 PUFAs and, in parallel, reduced n-3 PUFAs. This has led to decreased production of PGE2, which is believed to have a protective effect against inflammation of the airways. Conflicting hypotheses also concern vitamin D; both an excess and a deficiency of vitamin D, in fact, have been associated with an increased risk of asthma. Further studies on the role of these substances are necessary before any conclusions can be drawn on a clinical level. Astratto La crescente prevalenza negli ultimi decenni delle malattie allergiche in età pediatrica potrebbe essere legata a concomitanti cambiamenti nella dieta, in particolare alla minore e modificata introduzione di frutta, verdura e minerali. Il consumo di questi alimenti da parte delle donne in gravidanza e dei bambini nei primi anni di vita sembra essere associato ad un ridotto rischio di asma e di sintomi correlati. Gli alimenti che possono prevenire lo sviluppo di respiro sibilante (wheezing) attraverso i loro effetti antiossidanti contengono vitamina C e selenio; i livelli ematici di questi elementi sono correlati negativamente con il rischio di wheezing. Inoltre l'assunzione di vitamina E durante la gravidanza sembra essere correlato con un rischio ridotto di respiro sibilante per il nascituro. Allo stesso modo, basso apporto di zinco e di carotenoidi in donne in gravidanza è associata ad un aumentato rischio di wheezing e asma nell'infanzia. Anche le fibre hanno proprietà anti-infiammatorie ed effetti protettivi contro le malattie allergiche come la dermatite atopica e l’asma. Il consumo di grassi influenza lo sviluppo delle vie aeree. Le popolazioni dei paesi occidentali hanno aumentato il loro consumo di n-6 PUFA e, parallelamente, ridotto n-3 PUFA. Ciò ha portato alla diminuzione della produzione di PGE2, che si ritiene abbia un effetto protettivo contro l'infiammazione delle vie aeree. Ipotesi contrastanti riguardano la vitamina D, sia un eccesso che una carenza di vitamina D, infatti, sono stati associati ad un aumentato rischio di asma. Ulteriori studi sul ruolo di queste sostanze sono necessari prima di trarre conclusioni sul piano clinico. PMID:22651129

Management of patients with end-stage renal disease undergoing chemotherapy: recommendations of the Associazione Italiana di Oncologia Medica (AIOM) and the Società Italiana di Nefrologia (SIN).

PubMed

Pedrazzoli, Paolo; Silvestris, Nicola; Santoro, Antonio; Secondino, Simona; Brunetti, Oronzo; Longo, Vito; Mancini, Elena; Mariucci, Sara; Rampino, Teresa; Delfanti, Sara; Brugnatelli, Silvia; Cinieri, Saverio

2017-01-01

The overall risk of some cancers is increased in patients receiving regular dialysis treatment due to chronic oxidative stress, a weakened immune system and enhanced genomic damage. These patients could benefit from the same antineoplastic treatment delivered to patients with normal renal function, but a better risk/benefit ratio could be achieved by establishing specific guidelines. Key considerations are which chemotherapeutic agent to use, adjustment of dosages and timing of dialysis in relation to the administration of chemotherapy. We have reviewed available data present in the literature, including recommendations and expert opinions on cancer risk and use of chemotherapeutic agents in patients with end-stage renal disease. Experts selected by the boards of the societies provided additional information which helped greatly in clarifying some issues on which clear-cut information was missing or available data were conflicting. Data on the optimal use of chemotherapeutic agents or on credible schemes of polychemotherapy in haemodialysed patients are sparse and mainly derive from case reports or small case series. However, recommendations on dosing and timing of dialysis can be proposed for the most prescribed chemotherapeutic agents. The use of chemotherapeutic agents as single agents, or in combination, can be safely given in patients with end-stage renal disease. Appropriate dosage adjustments should be considered based on drug dialysability and pharmacokinetics. Coordinated care between oncologists, nephrologists and pharmacists is of pivotal importance to optimise drug delivery and timing of dialysis.

Calculation of streamflow statistics for Ontario and the Great Lakes states

USGS Publications Warehouse

Piggott, Andrew R.; Neff, Brian P.

2005-01-01

Basic, flow-duration, and n-day frequency statistics were calculated for 779 current and historical streamflow gages in Ontario and 3,157 streamflow gages in the Great Lakes states with length-of-record daily mean streamflow data ending on December 31, 2000 and September 30, 2001, respectively. The statistics were determined using the U.S. Geological Survey’s SWSTAT and IOWDM, ANNIE, and LIBANNE software and Linux shell and PERL programming that enabled the mass processing of the data and calculation of the statistics. Verification exercises were performed to assess the accuracy of the processing and calculations. The statistics and descriptions, longitudes and latitudes, and drainage areas for each of the streamflow gages are summarized in ASCII text files and ESRI shapefiles.

Unity nameplate examined before being attached to module for ISS and Mission STS-88

NASA Technical Reports Server (NTRS)

1998-01-01

Examining the nameplate for the Unity connecting module, in the Space Station Processing Facility, are (left to right) Joe Schweiger and Tommy Annis, of Boeing-KSC, and Nancy Tolliver, of Boeing-Huntsville. An unidentified worker behind them looks on. Part of the International Space Station, Unity was expected to be transported to Launch Pad 39A on Oct. 26 for launch aboard Space Shuttle Endeavour on Mission STS-88 in December. The Unity is a connecting passageway to the living and working areas of ISS. While on orbit, the flight crew will deploy Unity from the payload bay and attach Unity to the Russian-built Zarya control module which will be in orbit at that time.

KSC-2012-5016

NASA Image and Video Library

2012-09-06

CAPE CANAVERAL, Fla. – During NASA's Innovation Expo at the Kennedy Space Center in Florida, Annie Williams, of NASA's Medical and Environmental Management Division, proposes an innovation to recycling space hardware as art. Kennedy Kick-Start Chair Mike Conroy looks on from the left. As Kennedy continues developing programs and infrastructure to become a 21st century spaceport, many employees are devising ways to do their jobs better and more efficiently. On Sept. 6, 2012, 16 Kennedy employees pitched their innovative ideas for improving the center at the Kennedy Kick-Start event. The competition was part of a center-wide effort designed to increase exposure for innovative ideas and encourage their implementation. For more information, visit http://www.nasa.gov/centers/kennedy/news/kick-start_competition.html Photo credit: NASA/Gianni Woods

Epigenetic Testing for Breast Cancer Risk Stratification

DTIC Science & Technology

2012-10-01

The genes selected for this validation were: ER-POS: GSTP1 , HBA2, BNC1, and WDR66 ER-NEG: IRF7, PECI, ARTN, VCAN, ADM, LIPG, and PLAU Figure 1...6 maintained in only a small fraction of the tumor cells. Only BNC1, CCNA1, and GSTP1 show noticeable expansion of the methylated population in...ADM 0.820 0.609 ARTN 0.192 0.138 GSTP1 0.218 0.070 LIPG 0.090 0.011 CCNA1 0.655 0.428 VCAN 0.301 0.128 IRF7 0.483 0.496 HBA2 cND ND PLAU ND ND

Evaluation of Alternative Euthanasia Methods of Neonatal Chickens

PubMed Central

Gurung, Shailesh; White, Dima; Archer, Gregory; Zhao, Dan; Farnell, Yuhua; Byrd, J. Allen; Peebles, E. David

2018-01-01

Simple Summary Male layer chicks do not have economic value and are humanely killed after hatching. The layer industry is seeking alternative methods to humanely kill recently hatched male chicks. This study evaluated the use of gases or negative air pressure as a means of humane and viable alternatives to maceration. The treatments included carbon dioxide, nitrogen, reduced air pressure, and a negative control. The study showed that chicks exposed to treatments, gases or negative air pressure, did not differ significantly in terms of the physiological stress response. The use of carbon dioxide resulted in a faster onset of unconsciousness and ultimately death as compared to nitrogen or negative air pressure treatments. Abstract Hatched male layer chicks are currently euthanized by maceration in the United States. Public concerns on the use of maceration have led to the search for alternative methods. We hypothesized that gas inhalation and low atmospheric pressure stunning (LAPS) are viable and humane alternatives to instantaneous mechanical destruction. The objective of this study was to evaluate the physiological and behavioral responses of recently hatched male layer chicks when subjected to carbon dioxide, nitrogen inhalation, or LAPS. The study consisted of seven treatments: breathing air (NEG), 25% carbon dioxide (CO2), 50% CO2, 75% CO2, 90% CO2, 100% nitrogen (N2), or LAPS. Ten day-of-hatch, male layer chicks were randomly assigned to each treatment, and each treatment was replicated on ten different days. A custom-made vacuum system was used to reduce air pressure inside the chamber from 100.12 kPa to 15.3 kPa for the LAPS treatment. Serum corticosterone and serotonin levels were measured using commercially available competitive enzyme linked immunosorbent assay (ELISA). Latencies to loss of posture and motionlessness were determined from video recordings. The 25% and 50% CO2 treatments were discontinued after the first replication, as the majority of the chicks recovered. The chicks in the negative (NEG) group had significantly higher levels of corticosterone than the other four euthanasia treatments. On the other hand, the serotonin levels of chicks in the NEG group was significantly lower when compared to the other four euthanasia treatments. The latencies to loss of posture and motionlessness of chicks exposed to 75% and 90% CO2 were significantly shorter than those in the LAPS and N2 inhalation treatments. These data suggest that the stress responses of chicks to the CO2, N2, and LAPS treatments do not differ among each other. However, the CO2 inhalation method was faster in inducing loss of posture and motionlessness in chicks than the LAPS and N2 inhalation treatments. PMID:29522442

Italian biophysics and SIBPA speed-up the pace towards the long and winding road of the interdisciplinary science.

PubMed

Giacomazza, Daniela; Musio, Carlo

2016-01-01

This Special Issue of Biophysical Chemistry presents a selection of the contributions presented at the XXII National Congress of the Italian Society of Pure and Applied Biophysics (i.e., SIBPA, Società Italiana di Biofisica Pura ed Applicata) held on September 2014 in Palermo, Italy. Topics cover all biophysical disciplines, from molecular to cellular, to integrative biophysics giving a comprehensive view of the inter- and multi-disciplinary approach of modern biophysics. SIBPA, which turned 40 in 2013, continues to grow and attract interest.

Colorectal tumors: the histology report.

PubMed

Lanza, Giovanni; Messerini, Luca; Gafà, Roberta; Risio, Mauro

2011-03-01

Epithelial colorectal tumors are common pathologic entities. Their histology report should be comprehensive of a series of pathologic parameters essential for the correct clinical management of the patients. Diagnostic histologic criteria of adenomatous, serrated, inflammatory, and hamartomatous polyps and of polyposis syndromes are discussed. In addition, the pathologic features of early and advanced colorectal cancer are described and a checklist is given. Finally, molecular prognostic and predictive factors currently employed in the treatment of colorectal cancer are discussed. Copyright © 2011 Editrice Gastroenterologica Italiana S.r.l. Published by Elsevier Ltd.. All rights reserved.

Historical Archives in Italian Astronomical Observatories: The ``Specola 2000'' Project

NASA Astrophysics Data System (ADS)

Chinnici, I.; Mandrino, A.; Bònoli, F.

2006-12-01

Italy's well-consolidated tradition in astronomy is fully witnessed by its rich archival heritage. Astronomical records are stored in many observatories and universities, as well as in libraries and in private institutions. In 2000 a project was promoted to arrange and produce inventories of all material kept in Italian observatory archives. The project was planned by the Società Astronomica Italiana, and financial support was provided by the Italian Ministero per i Beni e le Attività Culturali. In this paper, the results obtained thus far are presented and commented on.

Selected Bibliography on Fiber-Reinforced Cement and Concrete. Supplement Number 4.

DTIC Science & Technology

1982-08-01

Building Industry," L’Industria Italiana del Cemento , Vol 50, No. 12, Dec 1980, pp 1135-1144. 19. Bartos, P., "Pullout Failure of Fibres Embedded in Cement...Vol 43, No. 11, Nov 1977, pp 561-564. 21. Bassan, M., "Model of Behavior of Fiber-Reinforced Concretes Under Impact Stresses," il Cemento , Vol 74, No...Pastes," il Cemento , Vol 75, No. 3, Jul-Sep 1978, pp 277-284. 210. Mills, R. H., "Age-Embrittlement of Glass-Reinforced Concrete Containing Blastfurance

[Atherosclerosis, chronic inflammation and oxidative stress in CKD].

PubMed

Leoni, Marco; Gorini, Antonio

2017-03-01

Chronic low-grade inflammation is emerging as the pathophysiological mechanism underlying of the several chronic degenerative diseases. Atherosclerosis, inflammation and oxidative stress are some of the issues that arise from the general context of chronic inflammation. In this manuscript we analyzed the role of the immune system, metabolism and inflammation's molecular mediators in order to show an overview about only apparently different problems. Finally, we proposed some possible solutions to improve the survival and quality of life of patient with chronic kidney disease. Copyright by Società Italiana di Nefrologia SIN, Rome, Italy.

Symposium on Signal and Image Processing English-Language Abstracts (12th) Held in Juan-Les-Pins, France on 12-16 June 1989

DTIC Science & Technology

1989-12-01

Neril1" (’)INFOCOM Dpt., via Eudossiana 18, 1-00184 Roma, Italy (2) CONTRAVES Italiana SpA. via Affile 102, 1-00139 Roma, Italy SUMMARY The paper...central processor. This makes the perception of the system less accurate and induces a loss in performance. Previous studies have considered the case...current practice. An inner code, often decoded using a weighted input, is concatenated with an outer code decoded without such a weighting . If

Divergent impact of Toll-like receptor 2 deficiency on repair mechanisms in healthy muscle versus Duchenne muscular dystrophy.

PubMed

Mojumdar, Kamalika; Giordano, Christian; Lemaire, Christian; Liang, Feng; Divangahi, Maziar; Qureshi, Salman T; Petrof, Basil J

2016-05-01

Injury to skeletal muscle, whether acute or chronic, triggers macrophage-mediated innate immunity in a manner which can be either beneficial or harmful for subsequent repair. Endogenous ligands for Toll-like receptor 2 (TLR2) are released by damaged tissues and might play an important role in activating the innate immune system following muscle injury. To test this hypothesis, we compared macrophage behaviour and muscle repair mechanisms in mice lacking TLR2 under conditions of either acute (cardiotoxin-induced) or chronic (mdx mouse genetic model of Duchenne muscular dystrophy; DMD) muscle damage. In previously healthy muscle subjected to acute damage, TLR2 deficiency reduced macrophage numbers in the muscle post-injury but did not alter the expression pattern of the prototypical macrophage polarization markers iNOS and CD206. In addition, there was abnormal persistence of necrotic fibres and impaired regeneration in TLR2-/- muscles after acute injury. In contrast, TLR2 ablation in chronically diseased muscles of mdx mice not only resulted in significantly reduced macrophage numbers but additionally modified their phenotype by shifting from inflammatory (iNOS(pos) CD206(neg) ) to more anti-inflammatory (iNOS(neg) CD206(pos) ) characteristics. This decrease in macrophage-mediated inflammation was associated with ameliorated muscle histopathology and improved force-generating capacity of the dystrophic muscle. Our results suggest that the role of TLR2 in macrophage function and skeletal muscle repair depends greatly upon the muscle injury context, and raise the possibility that inhibition of TLR2 could serve as a useful therapeutic measure in DMD. Copyright © 2016 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.

Negation handling in sentiment classification using rule-based adapted from Indonesian language syntactic for Indonesian text in Twitter

NASA Astrophysics Data System (ADS)

Amalia, Rizkiana; Arif Bijaksana, Moch; Darmantoro, Dhinta

2018-03-01

The presence of the word negation is able to change the polarity of the text if it is not handled properly it will affect the performance of the sentiment classification. Negation words in Indonesian are ‘tidak’, ‘bukan’, ‘belum’ and ‘jangan’. Also, there is a conjunction word that able to reverse the actual values, as the word ‘tetapi’, or ‘tapi’. Unigram has shortcomings in dealing with the existence of negation because it treats negation word and the negated words as separate words. A general approach for negation handling in English text gives the tag ‘NEG_’ for following words after negation until the first punctuation. But this may gives the tag to un-negated, and this approach does not handle negation and conjunction in one sentences. The rule-based method to determine what words negated by adapting the rules of Indonesian language syntactic of negation to determine the scope of negation was proposed in this study. With adapting syntactic rules and tagging “NEG_” using SVM classifier with RBF kernel has better performance results than the other experiments. Considering the average F1-score value, the performance of this proposed method can be improved against baseline equal to 1.79% (baseline without negation handling) and 5% (baseline with existing negation handling) for a dataset that all tweets contain negation words. And also for the second dataset that has the various number of negation words in document tweet. It can be improved against baseline at 2.69% (without negation handling) and 3.17% (with existing negation handling).

Ph+ ALL patients in first complete remission have similar survival after reduced intensity and myeloablative allogeneic transplantation: impact of tyrosine kinase inhibitor and minimal residual disease.

PubMed

Bachanova, V; Marks, D I; Zhang, M-J; Wang, H; de Lima, M; Aljurf, M D; Arellano, M; Artz, A S; Bacher, U; Cahn, J-Y; Chen, Y-B; Copelan, E A; Drobyski, W R; Gale, R P; Greer, J P; Gupta, V; Hale, G A; Kebriaei, P; Lazarus, H M; Lewis, I D; Lewis, V A; Liesveld, J L; Litzow, M R; Loren, A W; Miller, A M; Norkin, M; Oran, B; Pidala, J; Rowe, J M; Savani, B N; Saber, W; Vij, R; Waller, E K; Wiernik, P H; Weisdorf, D J

2014-03-01

The efficacy of reduced intensity conditioning (RIC) allogeneic hematopoietic cell transplantation (HCT) for Philadelphia chromosome positive (Ph+) acute lymphoblastic leukemia (ALL) is uncertain. We analyzed 197 adults with Ph+ ALL in first complete remission; 67 patients receiving RIC were matched with 130 receiving myeloablative conditioning (MAC) for age, donor type and HCT year. Over 75% received pre-HCT tyrosine kinase inhibitors (TKIs), mostly imatinib; 39% (RIC) and 49% (MAC) were minimal residual disease (MRD)(neg) pre-HCT. At a median 4.5 years follow-up, 1-year transplant-related mortality (TRM) was lower in RIC (13%) than MAC (36%; P=0.001) while the 3-year relapse rate was 49% in RIC and 28% in MAC (P=0.058). Overall survival (OS) was similar (RIC 39% (95% confidence interval (CI) 27-52) vs 35% (95% CI 27-44); P=0.62). Patients MRD(pos) pre-HCT had higher risk of relapse with RIC vs MAC (hazard ratio (HR) 1.97; P=0.026). However, patients receiving pre-HCT TKI in combination with MRD negativity pre-RIC HCT had superior OS (55%) compared with a similar MRD population after MAC (33%; P=0.0042). In multivariate analysis, RIC lowered TRM (HR 0.6; P=0.057), but absence of pre-HCT TKI (HR 1.88; P=0.018), RIC (HR 1.891; P=0.054) and pre-HCT MRD(pos) (HR 1.6; P=0.070) increased relapse risk. RIC is a valid alternative strategy for Ph+ ALL patients ineligible for MAC and MRD(neg) status is preferred pre-HCT.

High performance batch production of LREBa 2Cu 3O y using novel thin film Nd-123 seed

NASA Astrophysics Data System (ADS)

Muralidhar, M.; Suzuki, K.; Fukumoto, Y.; Ishihara, A.; Tomita, M.

2011-11-01

A batch production for fabrication of LREBa2Cu3Oy (LRE: Sm, Gd, NEG) "LRE-123" pellets are developed in air and Ar-1% O2 using a novel thin film Nd-123 seeds grown on MgO crystals. The SEM and XRD results conformed that the quality and orientation of the seed crystals are excellent. On the other hand, new seeds can withstand temperatures >1100 °C, as a result, the cold seeding process was applied even to grow Sm-123 material in Air. The trapped field observed in the best 45 mm single-grain puck of Gd-123 was in the range of 1.35 T and 0.35 T at 77.3 K and 87.3 K, respectively. The average trapped field at 77.3 K in the 24 mm diameter NEG-123 samples batch lies between 0.9 and 1 T. The maximum trapped field of 1.2 T was recorded at the sample surface. Further, the maximum trapped field of 0.23 T at 77 K was recorded in a sample with 16 mm diameter of Sm-123 with 3 mol% BaO2 addition. As a result we made more then 130 single grain pucks within a couple of months. Taking advantage of the single grain batch processed material, we constructed self-made chilled levitation disk, which was used on the open day of railway technical research Institute. More then 150 children stood on the levitation disk and revel the experience of levitation. The present results prove that a high-performance good-quality class of LREBa2Cu3Oy material can be made by using a novel thin film Nd-123 seeds.

Toxoplasma gondii impairs memory in infected seniors.

PubMed

Gajewski, Patrick D; Falkenstein, Michael; Hengstler, Jan G; Golka, Klaus

2014-02-01

Almost 30% of humans present a Toxoplasma gondii positive antibody status and its prevalence increases with age. The central nervous system is the main target. However, little is known about the influence of asymptomatic i.e. latent Toxoplasmosis on cognitive functions in humans. To investigate neurocognitive dysfunctions in asymptomatic older adults with T. gondii positive antibody status a double-blinded neuropsychological study was conducted. The participants were classified from a population-based sample (N=131) of healthy participants with an age of 65 years and older into two groups with 42 individuals each: Toxoplasmosis positive (T-pos; IgG>50 IU/ml) and Toxoplasmosis negative (T-neg; IgG=0 IU/ml). The outcome measures were a computer-based working-memory test (2-back) and several standardized psychometric tests of memory and executive cognitive functions. T-pos seniors showed an impairment of different aspects of memory. The rate of correctly detected target symbols in a 2-back task was decreased by nearly 9% (P=0.020), corresponding to a performance reduction of about 35% in working memory relative to the T-neg group. Moreover, T-pos seniors had a lower performance in a verbal memory test, both regarding immediate recall (10% reduction; P=0.022), delayed recognition (6%; P=0.037) and recall from long-term memory assessed by the word fluency tests (12%; P=0.029). In contrast, executive functions were not affected. The effects remained mostly unchanged after controlling for medication. The impairment of memory functions in T-pos seniors was accompanied by a decreased self-reported quality of life. Because of the high prevalence of asymptomatic Toxoplasmosis and an increasing population of older adults this finding is of high relevance for public health. Copyright © 2013 Elsevier Inc. All rights reserved.

Do Running Kinematic Characteristics Change over a Typical HIIT for Endurance Runners?

PubMed

García-Pinillos, Felipe; Soto-Hermoso, Víctor M; Latorre-Román, Pedro Á

2016-10-01

García-Pinillos, F, Soto-Hermoso, VM, and Latorre-Román, PÁ. Do running kinematic characteristics change over a typical HIIT for endurance runners?. J Strength Cond Res 30(10): 2907-2917, 2016-The purpose of this study was to describe kinematic changes that occur during a common high-intensity intermittent training (HIIT) session for endurance runners. Twenty-eight male endurance runners participated in this study. A high-speed camera was used to measure sagittal-plane kinematics at the first and the last run during a HIIT (4 × 3 × 400 m). The dependent variables were spatial-temporal variables, joint angles during support and swing, and foot strike pattern. Physiological variables, rate of perceived exertion, and athletic performance were also recorded. No significant changes (p ≥ 0.05) in kinematic variables were found during the HIIT session. Two cluster analyses were performed, according to the average running pace-faster vs. slower, and according to exhaustion level reached-exhausted group vs. nonexhausted group (NEG). At first run, no significant differences were found between groups. As for the changes induced by the running protocol, significant differences (p ≤ 0.05) were found between faster and slower athletes at toe-off in θhip and θknee, whereas some changes were found in NEG in θhip during toe-off (+4.3°) and θknee at toe-off (-5.2°) during swing. The results show that a common HIIT session for endurance runners did not consistently or substantially perturb the running kinematics of trained male runners. Additionally, although some differences between groups have been found, neither athletic performance nor exhaustion level reached seems to be determinant in the kinematic response during a HIIT, at least for this group of moderately trained endurance runners.

Repopulating hematopoietic stem cells from steady-state blood before and after ex vivo culture are enriched in CD34+CD133+CXCR4low fraction.

PubMed

Lapostolle, Véronique; Chevaleyre, Jean; Duchez, Pascale; Rodriguez, Laura; Vlaski-Lafarge, Marija; Sandvig, Ioanna; Brunet de la Grange, Philippe; Ivanovic, Zoran

2018-06-01

Feasibility of ex vivo expansion allows us to consider the steady-state peripheral blood as an alternative source of hematopoietic stem progenitor cells for transplantation when growth factor-induced cell mobilization is contraindicated or inapplicable. Ex vivo expansion dramatically enhances the in vivo reconstituting cell population from steady-state blood. In order to investigate phenotype and the expression of homing molecules, CD34, CD133, CD90, CD45RA, CD26 and CD9 expression was determined on sorted CD34+ cells according to CXCR4 (neg, low, bright) and CD133 expression before and after ex vivo expansion. Hematopoietic stem cell activity was determined in vivo on the basis of hematopoietic repopulation of primary and secondary recipients - NSG immuno-deficient mice. In vivo reconstituting cells in steady-state blood CD34+ cell fraction before expansion belong to the CD133+ population and are CXCR4low or, to a lesser extent, CXCR4neg, while after ex vivo expansion they are contained in only the CD133+CXCR4low cells. The failure of CXCR4bright population to engraft is probably due to the exclusive expression of CD26 by these cells. The limiting-dilution analysis showed that both repopulating cell number and individual proliferative capacity were enhanced by ex vivo expansion. Thus, steady-state peripheral blood cells exhibit a different phenotype compared to mobilized and cord blood ones, as well as to those issued from the bone marrow. This data represent the first phenotypic characterization of steady-state blood cells exhibiting short and long term hematopoietic reconstituting potential, which can be expanded ex vivo, a sine qua non for their subsequent use for transplantation. Copyright © 2018, Ferrata Storti Foundation.

Psychological stress is associated with altered levels of myeloid-derived suppressor cells in breast cancer patients.

PubMed

Mundy-Bosse, Bethany L; Thornton, Lisa M; Yang, Hae-Chung; Andersen, Barbara L; Carson, William E

2011-01-01

Our group has shown in a randomized clinical trial that psychological intervention to reduce stress in patients with stages II and III breast cancer led to enhanced immune function, fewer recurrences and improved overall survival. We hypothesized that patients with high levels of stress would have alterations in myeloid-derived suppressor cells (MDSC) compared to patients with lower stress. PBMC from 16 patients with high stress (n = 8) or with low stress (n = 8) after surgery as measured by the Impact of Event Scale (IES) questionnaire were evaluated for the presence of MDSC. Patients with higher IES scores had significantly elevated salivary cortisol levels (P = 0.013; 13 μg/dl vs. 9.74 μg/dl). Levels of IL-1Rα were also significantly elevated in the higher IES group (45.09 pg/ml vs. 97.16 pg/ml; P = 0.010). IP 10, G-CSF, and IL-6 were all higher in the high stress group although not to a significant degree. Flow cytometric analysis for CD33+/HLA-DR-neg/CD15+/CD11b+ MDSC revealed increased MDSC in patients with lower IES scores (P = 0.009). CD11b+/CD15+ cells constituted 9.4% of the CD33+/HLA DR-neg cell population in patients with high IES, vs. 27.3% in patients with low IES scores. Additional analyzes of the number of stressful events that affected the patients in addition to their cancer diagnosis revealed that this type of stress measure correlated with elevated levels of MDSC (P = 0.064). These data indicate the existence of a complex relationship between stress and immune function in breast cancer patients. Copyright © 2011 Elsevier Inc. All rights reserved.

Psychological Stress is Associated with Altered Levels of Myeloid-Derived Suppressor cells in Breast Cancer Patients

PubMed Central

Mundy-Bosse, Bethany L.; Thornton, Lisa M.; Yang, Hae-Chung; Andersen, Barbara L.; Carson, William E.

2011-01-01

Our group has shown in a randomized clinical trial that psychological intervention to reduce stress in patients with stage II and III breast cancer led to enhanced immune function, fewer recurrences and improved overall survival. We hypothesized that patients with high levels of stress would have alterations in myeloid-derived suppressor cells (MDSC) compared to patients with lower stress. PBMC from 16 patients with high stress (n = 8) or with low stress (n = 8) after surgery as measured by the Impact of Event Scale (IES) questionnaire were evaluated for the presence of MDSC. Patients with higher IES scores had significantly elevated salivary cortisol levels (P = 0.013; 13 µg/dl vs. 9.74 µg/dl). Levels of IL-1Rα were also significantly elevated in the higher IES group (45.09 pg/mL vs. 97.16 pg/mL; P = 0.010). IP 10, G-CSF, and IL-6 were all higher in the high stress group although not to a significant degree. Flow cytometric analysis for CD33+/HLA-DR-neg/CD15+/CD11b+ MDSC revealed increased MDSC in patients with lower IES scores (P = 0.009). CD11b+/CD15+ cells constituted 9.4% of the CD33+/HLA DR-neg cell population in patients with high IES, versus 27.3% in patients with low IES scores. Additional analyses of the number of stressful events that affected the patients in addition to their cancer diagnosis revealed that this type of stress measure correlated with elevated levels of MDSC (P = .064). These data indicate the existence of a complex relationship between stress and immune function in breast cancer patients. PMID:21600570

Anti-citrullinated protein antibodies contribute to platelet activation in rheumatoid arthritis.

PubMed

Habets, Kim L L; Trouw, Leendert A; Levarht, E W Nivine; Korporaal, Suzanne J A; Habets, Petra A M; de Groot, Philip; Huizinga, Tom W J; Toes, René E M

2015-08-24

Although the role of platelets in rheumatoid arthritis (RA) is relatively unexplored, recent studies point towards a contribution of platelets in arthritis. We set out to determine platelet phenotype in RA and studied whether this could be influenced by the presence of anti-citrullinated protein antibodies (ACPA). Platelets from healthy controls were incubated in the presence of plasma of patients with RA or age- and sex-matched healthy controls and plasma from ACPA(neg) or ACPA(pos) patients or in the presence of plate-bound ACPA. Characteristics of platelets isolated from patients with RA were correlated to disease activity. Platelets isolated from healthy controls displayed markers of platelet activation in the presence of plasma derived from RA patients, as determined by P-selectin expression, formation of aggregates and secretion of soluble CD40 ligand (sCD40L). Furthermore, levels of P-selectin expression and sCD40L release correlated with high ACPA titres. In accordance with these findings, enhanced platelet activation was observed after incubation with ACPA(pos) plasma versus ACPA(neg) plasma. Pre-incubation of platelets with blocking antibodies directed against low-affinity immunoglobulin G receptor (FcγRIIa) completely inhibited the ACPA-mediated activation. In addition, expression of P-selectin measured as number of platelets correlated with Disease Activity Score in 44 joints, C-reactive protein level, ACPA status and ACPA level. We show for the first time that ACPA can mediate an FcγRIIa-dependent activation of platelets. As ACPA can be detected several years before RA disease onset and activated platelets contribute to vascular permeability, these data implicate a possible role for ACPA-mediated activation of platelets in arthritis onset.

Prevalence, prognosis, and treatment implications of retropharyngeal nodes in unknown primary head and neck carcinoma.

PubMed

Horowitz, Gilad; Hosni, Ali; Yu, Eugene; Xu, Wei; Lu, Lin; Au, Michael; Dixon, Peter R; Brown, Dale; Chepeha, Douglas B; Gilbert, Ralph W; Goldstein, David P; Gullane, Patrick J; Irish, Jonathan C; Bayley, Andrew; Cho, John; Giuliani, Meredith; Huang, Shao Hui; Hope, Andrew; Kim, John; O'Sullivan, Brian; Ringash, Jolie; Waldron, John; Weinreb, Ilan; Perez-Ordonez, Bayardo; Bratman, Scott V; de Almeida, John R

2018-07-01

(1) To estimate the prevalence of radiographically positive Retro-Pharyngeal Lymph Nodes (RPLN) in unknown primary carcinoma of the head and neck and (2) to determine the prognostic implications of radiographically positive RPLN and other radiographic features (3) to identify patients at low risk for retropharyngeal metastasis. The medical records of all 68 eligible patients treated at the Princess Margaret Cancer Centre between 2000 and 2014 were retrospectively reviewed for demographic, clinical, pathologic, and radiologic data. Radiologic data included: RPLN, extra capsular spread (ECS), neck staging and cystic/necrotic or matted neck nodes. LRR, DR, DFS and OS were estimated using the competing risk methods and the Kaplan-Meier method. Seven patients had concerning RPLN (10.3%). Forty-four patients were p16 positive (65%). RPLN status did not have any effect on LRR, DFS, DR and OS. Radiological ECS and p16 (neg.) status were found to be significant predictors of LRR (p = 0.023; p = 0.014). Matted nodes, radiological ECS and p16 (neg.) status were found to be significant predictors of DFS (p = 0.012; p < 0.001; p = 0.014). Matted nodes and radiological ECS were found to be significant predictors of OS (p = 0.017; p = 0.0036). Only radiological ECS was found to be a significant predictor of distant recurrence (p = 0.0066). 10% of CUP patients will harbor radiological positive RPLN. A large proportion of CUP patients are positive for p16. Radiologic features such as ECS and matted nodes can predict worse outcomes. Copyright © 2018. Published by Elsevier Ltd.

Health Authorities Data Collection of THC:CBD Oromucosal Spray (L'Agenzia Italiana del Farmaco Web Registry): Figures after 1.5 Years.

PubMed

Patti, Francesco

2016-01-01

In Italy, all prescriptions for THC:CBD oromucosal spray for treatment of multiple sclerosis (MS) spasticity are linked to the official Agenzia Italiana del Farmaco (AIFA) web-based registry, which tracks the effectiveness and tolerability of medications in a prospective and observational manner. AIFA e-registry data for THC:CBD oromucosal spray collected between January 2014 and February 2015 for 1,534 patients from 30 large Italian specialized MS centres were compiled. Patients had a long disease history (17.6 ± 8.6 years) and significant impairment (mean Expanded Disability Status Scale score 6.4 ± 1.2). MS spasticity was evaluated using the 0-10 numerical rating scale (NRS). After the first month titration and trial period, 61.9% of patients achieved sufficient improvement in spasticity (≥20% NRS) to qualify for continued treatment. After 6 months, clinically meaningful ≥30% NRS improvement was recorded in 40.2% of patients continuing with treatment. Spasticity-associated symptoms such as cramps and nocturnal spasms improved in most responding patients. Mean reported doses of THC:CBD oromucosal spray (6.2-6.7 sprays/day) were lower than those reported in clinical trials. Adverse events (mainly mild to moderate) were reported by 15% of patients; no new safety concerns beyond the approved label were identified. The results of the AIFA e-registry analysis align with those of other THC:CBD observational projects and reaffirm the characteristics of this therapeutic option in the management of treatment-resistant MS spasticity, a frequently overlooked symptom. © 2016 S. Karger AG, Basel.

Management of patients with end-stage renal disease undergoing chemotherapy: recommendations of the Associazione Italiana di Oncologia Medica (AIOM) and the Società Italiana di Nefrologia (SIN)

PubMed Central

Pedrazzoli, Paolo; Silvestris, Nicola; Santoro, Antonio; Secondino, Simona; Brunetti, Oronzo; Longo, Vito; Mancini, Elena; Mariucci, Sara; Rampino, Teresa; Delfanti, Sara; Brugnatelli, Silvia; Cinieri, Saverio

2017-01-01

Background The overall risk of some cancers is increased in patients receiving regular dialysis treatment due to chronic oxidative stress, a weakened immune system and enhanced genomic damage. These patients could benefit from the same antineoplastic treatment delivered to patients with normal renal function, but a better risk/benefit ratio could be achieved by establishing specific guidelines. Key considerations are which chemotherapeutic agent to use, adjustment of dosages and timing of dialysis in relation to the administration of chemotherapy. Methods We have reviewed available data present in the literature, including recommendations and expert opinions on cancer risk and use of chemotherapeutic agents in patients with end-stage renal disease. Experts selected by the boards of the societies provided additional information which helped greatly in clarifying some issues on which clear-cut information was missing or available data were conflicting. Results Data on the optimal use of chemotherapeutic agents or on credible schemes of polychemotherapy in haemodialysed patients are sparse and mainly derive from case reports or small case series. However, recommendations on dosing and timing of dialysis can be proposed for the most prescribed chemotherapeutic agents. Discussion The use of chemotherapeutic agents as single agents, or in combination, can be safely given in patients with end-stage renal disease. Appropriate dosage adjustments should be considered based on drug dialysability and pharmacokinetics. Coordinated care between oncologists, nephrologists and pharmacists is of pivotal importance to optimise drug delivery and timing of dialysis. PMID:29209521

A reflection on the work of Gianni Bonadonna from the viewpoint of the global challenge of adolescents and young adults with cancer.

PubMed

Barr, Ronald D; Bleyer, W Archie

2017-11-23

Adolescents and young adults (AYAs - ages 15 to 39) constitute approximately 40% of the world's population and contribute an estimated one million new cases of cancer annually, the great majority in low- and middle-income countries (LMICs). In high-income countries (HICs) cancer is the commonest cause of disease-related death in AYAs, though overall 5-year survival rates now exceed 80%. A very different circumstance likely holds in LMICs, but accurate assessments are not readily available.Breast cancer accounts for 40% of tumours in female AYAs and this age group includes the peak incidence of Hodgkin lymphoma. The late Professor Gianni Bonadonna contributed importantly to improved survival in patients with these two diseases. Accordingly, he would be justifiably proud of the advances in AYA oncology that are being made in Italy, especially the impact of his colleagues at the Istituto Nazionale dei Tumori (INT). The initiatives of the Associazione Italiana Ematologia Pediatrica and the Società Italiana Adolescenti con Malattie Onco-ematologiche are particularly noteworthy, with the accomplishment of productive collaboration between paediatric and adult cancer care providers serving as a model for other countries to emulate.Exporting these advances can be successful through the vehicle of "twinning": establishing sustainable cooperation between institutions in HICs and partners in LMICs. Colleagues in Monza and at INT have been leaders in such programmes for decades. Cancer in AYAs remains a global challenge to which Gianni Bonadonna surely would have risen with enthusiasm and leadership while securing measurable achievements.

Panel established to revise position statement on climate change

NASA Astrophysics Data System (ADS)

President Robert Dickinson has appointed a panel to review the current AGU position statement on climate change and greenhouse gases, and to consider revising the statement to reflect scientific progress over the last four years. Marvin Geller of the State University of New York-Stonybrook chairs the panel.Other panel members include: Andre Berger, George Lemaître Catholic University of Louvain, Belgium; Anny Cazenave, Observatoire Midi-Pyrénées, Toulouse, France; John Christy, University of Alabama, Huntsville; Ellen Druffel, University of California, Irvine; Jack Fellows, University Consortium for Atmospheric Research, Boulder; Hiroshi Kanzawa, Nagoya University, Japan; William Schlesinger, Duke University, Durham; William (Jim) Shuttleworth, University of Arizona; Eric Sundquist, U.S. Geological Survey, Woods Hole; Richard Turco, University of California, Los Angeles; Ilana Wainer, Universidade Cidade Sao Paulo, Brazil.

[Darwin and bacteria].

PubMed

Ledermann D, Walter

2009-02-01

As in 2009 the scientific world celebrates two hundreds years from the birthday of Charles Darwin and one hundred and fifty from the publication of The Origin of Species, an analysis of his complete work is performed, looking for any mention of bacteria. But it seems that the great naturahst never took knowledge about its existence, something rather improbable in a time when the discovery of bacteria shook the medical world, or he deliberately ignored them, not finding a place for such microscopic beings into his theory of evolution. But the bacteria badly affected his familiar life, killing scarlet fever one of his children and worsening to death the evolution of tuberculosis of his favourite Annie. Darwin himself could suffer the sickness of Chagas, whose etiological agent has a similar level to bacteria in the scale of evolution.

A Summary of the NASA ISS Space Debris Collision Avoidance Program

NASA Technical Reports Server (NTRS)

Frisbee, Joseph

2002-01-01

Creating and implementing a process for the mitigation of the impact hazards due to cornets and asteroids will prove to be a complex and involved process. The closest similar program is the collision avoidance process currently used for protection of the International Space Station (ISS). This process, in operation for over three years, has many similarities to the NEG risk problem. By reviewing the ISS program, a broader perspective on the complications of and requirements for a NEO risk mitigation program might be obtained. Specifically, any lessons learned and continuing issues of concern might prove useful in the development of a NEO risk assessment and mitigation program.

Effects of air ions on the neonatal growth of laboratory rats

NASA Astrophysics Data System (ADS)

Hinsull, S. M.; Bellamy, D.; Head, E. L.

1981-12-01

The effect of continuous positive and negative ionization on the growth of rats during the pre and post natal period, up to 10 weeks of age was investigated. It was found that continuous exposure to 1.0×104 pos. ions/ml had no detrimental effect on the animals at any stage of their development. In contrast, exposure to 1.0×104 neg. ions/ml, during gestation and the early post natal period, resulted in some adverse effects on growth and development. However, when exposure to this level of negative ions began at the time of weaning, no adverse effects were observed.

Micro Computer Feedback Report for the Strategic Leader Development Inventory

DTIC Science & Technology

1993-05-01

POS or NEG variables CALL CREATE MEM DIR ;make a memory directory JC SELS ;exat I error CALL SELECT-SCREEN ;dlsplay select screen JC SEL4 ;no flles in...get keyboaI Input CMP AL,1Bh3 ;ls I an Esc key ? JNZ SEL2 ;X not goto nrod test G-95 JMP SEL4 ;Exit SEL2: CMP AL,OOh Iskapick? JZ SEL ;I YES exit loop...position CALL READ DATE ;gat DOS daoe od 4e CALL F4ND -ERO ;kxlae OW In data ue JC SEL.5 SEL4 : CALL RELEASE MEM DIR ;release meu block CLC ;cler carry fag

The pattern of name tokens in narrative clinical text and a comparison of five systems for redacting them

PubMed Central

Kayaalp, Mehmet; Browne, Allen C; Callaghan, Fiona M; Dodd, Zeyno A; Divita, Guy; Ozturk, Selcuk; McDonald, Clement J

2014-01-01

Objective To understand the factors that influence success in scrubbing personal names from narrative text. Materials and methods We developed a scrubber, the NLM Name Scrubber (NLM-NS), to redact personal names from narrative clinical reports, hand tagged words in a set of gold standard narrative reports as personal names or not, and measured the scrubbing success of NLM-NS and that of four other scrubbing/name recognition tools (MIST, MITdeid, LingPipe, and ANNIE/GATE) against the gold standard reports. We ran three comparisons which used increasingly larger name lists. Results The test reports contained more than 1 million words, of which 2388 were patient and 20 160 were provider name tokens. NLM-NS failed to scrub only 2 of the 2388 instances of patient name tokens. Its sensitivity was 0.999 on both patient and provider name tokens and missed fewer instances of patient name tokens in all comparisons with other scrubbers. MIST produced the best all token specificity and F-measure for name instances in our most relevant study (study 2), with values of 0.997 and 0.938, respectively. In that same comparison, NLM-NS was second best, with values of 0.986 and 0.748, respectively, and MITdeid was a close third, with values of 0.985 and 0.796 respectively. With the addition of the Clinical Center name list to their native name lists, Ling Pipe, MITdeid, MIST, and ANNIE/GATE all improved substantially. MITdeid and Ling Pipe gained the most—reaching patient name sensitivity of 0.995 (F-measure=0.705) and 0.989 (F-measure=0.386), respectively. Discussion The privacy risk due to two name tokens missed by NLM-NS was statistically negligible, since neither individual could be distinguished among more than 150 000 people listed in the US Social Security Registry. Conclusions The nature and size of name lists have substantial influences on scrubbing success. The use of very large name lists with frequency statistics accounts for much of NLM-NS scrubbing success. PMID:24026308

Work continues on Destiny, the U.S. Lab module, in the Space Station Processing Facility

NASA Technical Reports Server (NTRS)

1999-01-01

In the Space Station Processing Facility (SSPF), work continues on the U.S. Lab module, Destiny, which is scheduled to be launched on Space Shuttle Endeavour in early 2000. It will become the centerpiece of scientific research on the International Space Station. Destiny shares space in the SSPF with the Shuttle Radar Topography Mission (SRTM) and Leonardo, the Multipurpose Logistics Module (MPLM) built by the Agenzia Spaziale Italiana (ASI). The SRTM is targeted for launch on mission STS-99 in September 1999. Leonardo is scheduled to launch on mission STS- 102 in June 2000.

Carcinoma of the exocrine pancreas: the histology report.

PubMed

Capella, Carlo; Albarello, Luca; Capelli, Paola; Sessa, Fausta; Zamboni, Giuseppe

2011-03-01

The Italian Group of Gastrointestinal Pathologists has named a committee to develop recommendations concerning the surgical pathology report for pancreatic cancer. The committee, formed by individuals with special expertise, wrote the recommendations, which were reviewed and approved by council of the Group. The recommendations are divided into several areas including an informative gross description, gross specimen handling, histopathologic diagnosis, immunohistochemistry, molecular findings, and a checklist. The purpose of these recommendations is to provide a fully informative report for the clinician. Copyright © 2011 Editrice Gastroenterologica Italiana S.r.l. Published by Elsevier Ltd.. All rights reserved.

[Protein carbamylation: what it is and why it concerns nephrologists].

PubMed

De Simone, Emanuele; Di Micco, Lucia; La Manna, Gaetano; Di Iorio, Biagio

2018-05-01

Spontaneous urea dissociation in water solution is a prominent source of protein carbamylation in our body. Protein carbamylation is a well-known phenomenon since early seventies. Some years ago, much interest in the diagnostic power of carbamylated protein arouse. Recently the target of the researches focused on its potential cardiovascular pathogenicity. Some authors claimed that this could be a reason for higher cardiovascular mortality in uremic patients. Nutritional therapy, amino acids supplementation and intensive dialysis regimen are some of the therapeutic tools tested to lower the carbamylation burst in this population. Copyright by Società Italiana di Nefrologia SIN, Rome, Italy.

[Cardio-Pulmonary-Renal interactions].

PubMed

Samoni, Sara; Husain-Syed, Faeq; De Rosa, Silvia; Ronco, Claudio

2017-03-01

Over the past decade, understanding about feedback mechanisms involving the heart, lung and kidney is significantly improved. Each organ injury may trigger hemodynamic, neuro-hormonal and cellular pathway that may damage diverse organs. Recurrent acute on chronic injury may lead to the advanced stage of disease. On the other hand, chronic pathological conditions may decrease functional reserve leading to a high susceptibility to acute injury. Assessment of functional reserve and dosage of novel biomarkers may allow an early diagnosis and treatment. This review summarizes the current state-of-the-art understanding of cardio-pulmonary-renal interactions. Copyright by Società Italiana di Nefrologia SIN, Rome, Italy.

Marie Curie's contribution to Medical Physics.

PubMed

Jean-Claude, Rosenwald; Nüsslin, Fridtjof

2013-09-01

On occasion of its 50th anniversary, the International Organization for Medical Physics (IOMP) from now on is going to celebrate annually an International Day of Medical Physics for which the 7th November, the birthday of Marie Sklodowska Curie, a most exceptional character in science at all and a pioneer of medical physics, has been chosen. This article briefly outlines her outstanding personality, sketches her fundamental discovery of radioactivity and emphasizes the impact of her various achievements on the development of medical physics at large. © 2013 Published by Elsevier Ltd on behalf of Associazione Italiana di Fisica Medica.

Effects of energy supplementation on energy losses and nitrogen balance of steers fed green-chopped wheat pasture I: Calorimetry.

PubMed

Shreck, A L; Ebert, P J; Bailey, E A; Jennings, J S; Casey, K D; Meyer, B E; Cole, N A

2017-05-01

Cattle grazing wheat pasture in the southern Great Plains are sometimes fed an energy supplement; however, the benefits of supplementation on nutrient balance, energy metabolism, and greenhouse gas emissions have not been elucidated. Therefore, we used 10 British crossbred steers (206 ± 10.7 kg initial BW) in a respiration calorimetry study to evaluate the effects of energy supplementation on energy losses, N balance, and nutrient digestibility of steers fed green-chopped wheat forage. The study design was an incomplete replicated 4 × 4 Latin square with treatments in a 2 × 2 factorial arrangement. Steers ( = 8) were assigned to 1 of 2 BW blocks (4 steers per block) with dietary factors consisting of 1) no supplementation (CON) or supplemented with a steam-flaked corn-based energy supplement (that also contained monensin sodium) at 0.5% of BW daily (SUP) and 2) NEm intakes of 1 times (1x) or 1.5 times (1.5x) maintenance. Wheat forage was harvested daily and continuously fed as green-chop to steers during the 56-d study. There were no differences ( ≥ 0.32) between CON and SUP for OM (78.3 vs. 80.7%, respectively) or NDF (68.3 vs. 64.8%, respectively) digestibility. At the 1.5x level of intake, there was no difference ( ≥ 0.16) in energy lost in feces (4.27 vs. 3.92 Mcal/d) or urine (0.58 vs. 0.55 Mcal/d), heat production (8.69 vs. 8.44 Mcal/d), or retained energy (3.10 vs. 3.46 Mcal/d) between supplementation treatments. Oxygen consumption (1,777 vs. 1,731 L/d; = 0.67) and CO production (1,704 vs. 1,627 L/d; = 0.56) of CON and SUP steers, respectively, were not different; however, SUP steers tended to have ( = 0.06) lower CH production (115 vs 130 L/d) than CON steers. Methane, as a proportion of GE intake, was similar for CON (6.87%) and SUP (6.07%; = 0.18), as was the ME:DE ratio ( = 0.24; 86.3% for CON and 87.9% for SUP). Fractional N excretion in urine and feces, as a proportion of total N excreted ( ≥ 0.84) or N intake ( ≥ 0.63), was not different between treatments. Calculated NEm and NEg values for CON were 1.76 and 1.37 Mcal/kg DM, respectively, whereas the NEm and NEg values for the SUP treatment were 2.32 and 1.61 Mcal/kg DM, respectively. Calculated NE values for steers fed additional energy were approximately 17.5% greater than the expected difference in energy content. This was probably the result of the inconsistent response at the 1x DMI level. Under these circumstances, energy supplementation did appear to enhance NEm and NEg value of the supplemented wheat forage diet.

Poverty, philanthropy, and professionalism. The establishment of a district nursing service in Wellington, New Zealand, 1903.

PubMed

Wood, Pamela; Arcus, Kerri

2011-01-01

The establishment in 1903 of a professional district nursing service in Wellington, New Zealand's capital city, was a philanthropic response to the need for skilled care for the sickpoor in their own homes, as hospital and charitable aid boards believed chronic patients drained their resources. This paper argues that it was the timely combination of the individual philanthropy of Sarah Ann Rhodes, the organisational philanthropy of the St John Ambulance Association and the new professional standing and availability of registered nurses such as Annie Holgate that ensured its successful foundation. It also argues that district nursing services blurred spatial, social, and public-private boundaries in new ways. Finally, it considers the district nurse's role as the philanthropist 's proxy, the means for realising the philanthropist's desire to help the sick poor.

Alterations in MAST suit pressure with changes in ambient temperature.

PubMed

Sanders, A B; Meislin, H W; Daub, E

1983-01-01

A study was undertaken to test the hypothesis that change in ambient air temperature has an effect on MAST suit pressure according to the ideal gas law. Two different MAST suits were tested on Resusci-Annie dummies. The MAST suits were applied in a cold room at 4.4 degrees C and warmed to 44 degrees C. Positive linear correlations were found in nine trials, but the two suits differed in their rate of increase in pressure. Three trials using humans were conducted showing increased pressure with temperature but at a lesser rate than with dummies. A correlation of 0.5 to 1.0 mm Hg increase in MAST suit pressure for each 1.0 degrees C increase in ambient temperature was found. Implications are discussed for the use of the MAST suit in environmental conditions where the temperature changes.

Prevalence and Determinants of the Use of Lipid-Lowering Agents in a Population of Older Hospitalized Patients: the Findings from the REPOSI (REgistro POliterapie Società Italiana di Medicina Interna) Study.

PubMed

Bertolotti, Marco; Franchi, Carlotta; Rocchi, Marco B L; Miceli, Andrea; Libbra, M Vittoria; Nobili, Alessandro; Lancellotti, Giulia; Carulli, Lucia; Mussi, Chiara

2017-04-01

Older patients are prone to multimorbidity and polypharmacy, with an inherent risk of adverse events and drug interactions. To the best of our knowledge, available information on the appropriateness of lipid-lowering treatment is extremely limited. The aim of the present study was to quantify and characterize lipid-lowering drug use in a population of complex in-hospital older patients. We analyzed data from 87 units of internal medicine or geriatric medicine in the REPOSI (Registro Politerapie della Società Italiana di Medicina Interna) study, with reference to the 2010 and 2012 patient cohorts. Lipid-lowering drug use was closely correlated with the clinical profiles, including multimorbidity markers and polypharmacy. 2171 patients aged >65 years were enrolled (1057 males, 1114 females, mean age 78.6 years). The patients treated with lipid-lowering drugs amounted to 508 subjects (23.4%), with no gender difference. Atorvastatin (39.3%) and simvastatin (34.0%) were the most widely used statin drugs. Likelihood of treatment was associated with polypharmacy (≥5 drugs) and with higher Cumulative Illness Rating Scale (CIRS) score. At logistic regression analysis, the presence of coronary heart disease, peripheral vascular disease, and hypertension were significantly correlated with lipid-lowering drug use, whereas age showed an inverse correlation. Diabetes was not associated with drug treatment. In this in-hospital cohort, the use of lipid-lowering agents was mainly driven by patients' clinical history, most notably the presence of clinically overt manifestations of atherosclerosis. Increasing age seems to be associated with lower prescription rates. This might be indicative of cautious behavior towards a potentially toxic treatment regimen.

[About some medical bibliographical sources of Jacobinic and Napoleonic age].

PubMed

Porro, Alessandro; Franchini, Antonia Francesca; Falconi, Bruno

2011-01-01

The authors analyze some bibliographical sources of jacobinic and Napoleonic age: books and pamphlets published by Stamperia Italiana e Francese in Milan (Lombardy); the Bullettino del Consiglio Subalpino di SanitY, ossia Giornale Fisico-Medico del Piemonte in Turin (Piedmont); some Charles Botta (1766-1837) books (Storia naturale e medica dell 'isola di Corfù; Mémoire du [...] sur la doctrine de Brown; Vicissitudes de l'instruction publique en Piémont depuis l'an VII jusq'au mois de ventose an XI [...]). They are useful to analyse the spreading of John Brown (1735-1788) theories in Italy, during the jacobinic and napoleonic time.

Calculating and controlling the error of discrete representations of Pareto surfaces in convex multi-criteria optimization.

PubMed

Craft, David

2010-10-01

A discrete set of points and their convex combinations can serve as a sparse representation of the Pareto surface in multiple objective convex optimization. We develop a method to evaluate the quality of such a representation, and show by example that in multiple objective radiotherapy planning, the number of Pareto optimal solutions needed to represent Pareto surfaces of up to five dimensions grows at most linearly with the number of objectives. The method described is also applicable to the representation of convex sets. Copyright © 2009 Associazione Italiana di Fisica Medica. Published by Elsevier Ltd. All rights reserved.

Moderate and intense exercise lifestyles attenuate the effects of aging on telomere length and the survival and composition of T cell subpopulations.

PubMed

Silva, Léia Cristina Rodrigues; de Araújo, Adriana Ladeira; Fernandes, Juliana Ruiz; Matias, Manuella de Sousa Toledo; Silva, Paulo Roberto; Duarte, Alberto J S; Garcez Leme, Luiz Eugênio; Benard, Gil

2016-02-01

Studies indicate that exercise might delay human biological aging, but the effects of long-term exercise on T cell function are not well known. We tested the hypothesis that moderate or intense exercise lifestyle may attenuate the effects of aging on the telomere length and the survival and composition of T cell subpopulations. Elderly (65-85 years) with intense training lifestyle (IT, n = 15), moderate training lifestyle (MT, n = 16), and who never trained (NT, n = 15) were studied. Although the three groups presented the age-associated contraction of the TCD4(+)/TCD8(+) naïve compartments and expansion of the memory compartments, both training modalities were associated with lower proportion of terminally differentiated (CD45RA(+)CCR7(neg)) TCD4(+) and TCD8(+) cells, although among the latter cells, the reduction reached statistical significance only with IT. MT was associated with higher proportion of central memory TCD4(+) cells, while IT was associated with higher proportion of effector memory TCD8(+) cells. However, both training lifestyles were unable to modify the proportion of senescent (CD28(neg)) TCD8(+) cells. Telomeres were longer in T cells in both training groups; with IT, telomere length increased mainly in TCD8(+) cells, whereas with MT, a modest increase in telomere length was observed in both TCD8(+) and TCD4(+) cells. Reduced commitment to apoptosis of resting T cells, as assessed by caspase-3 and Bcl-2 expression, was seen predominantly with IT. Measurement of pro-inflammatory cytokines in serum and peripheral blood mononuclear cell (PBMC)'s supernatants did not show chronic low-grade inflammation in any of the groups. In conclusion, MT and IT lifestyles attenuated some of the effects of aging on the immune system.

Early Expansion of Circulating Granulocytic Myeloid-derived Suppressor Cells Predicts Development of Nosocomial Infections in Patients with Sepsis.

PubMed

Uhel, Fabrice; Azzaoui, Imane; Grégoire, Murielle; Pangault, Céline; Dulong, Joelle; Tadié, Jean-Marc; Gacouin, Arnaud; Camus, Christophe; Cynober, Luc; Fest, Thierry; Le Tulzo, Yves; Roussel, Mikael; Tarte, Karin

2017-08-01

Sepsis induces a sustained immune dysfunction responsible for poor outcome and nosocomial infections. Myeloid-derived suppressor cells (MDSCs) described in cancer and inflammatory processes may be involved in sepsis-induced immune suppression, but their clinical impact remains poorly defined. To clarify phenotype, suppressive activity, origin, and clinical impact of MDSCs in patients with sepsis. Peripheral blood transcriptomic analysis was performed on 29 patients with sepsis and 15 healthy donors. A second cohort of 94 consecutive patients with sepsis, 11 severity-matched intensive care patients, and 67 healthy donors was prospectively enrolled for flow cytometry and functional experiments. Genes involved in MDSC suppressive functions, including S100A12, S100A9, MMP8, and ARG1, were up-regulated in the peripheral blood of patients with sepsis. CD14 pos HLA-DR low/neg monocytic (M)-MDSCs were expanded in intensive care unit patients with and without sepsis and CD14 neg CD15 pos low-density granulocytes/granulocytic (G)-MDSCs were more specifically expanded in patients with sepsis (P < 0.001). Plasma levels of MDSC mediators S100A8/A9, S100A12, and arginase 1 were significantly increased. In vitro, CD14 pos - and CD15 pos -cell depletion increased T-cell proliferation in patients with sepsis. G-MDSCs, made of immature and mature granulocytes expressing high levels of degranulation markers, were specifically responsible for arginase 1 activity. High initial levels of G-MDSCs, arginase 1, and S100A12 but not M-MDSCs were associated with subsequent occurrence of nosocomial infections. M-MDSCs and G-MDSCs strongly contribute to T-cell dysfunction in patients with sepsis. More specifically, G-MDSCs producing arginase 1 are associated with a higher incidence of nosocomial infections and seem to be major actors of sepsis-induced immune suppression.

Anti-oxidant effect of bergamot polyphenolic fraction counteracts doxorubicin-induced cardiomyopathy: Role of autophagy and c-kitposCD45negCD31neg cardiac stem cell activation.

PubMed

Carresi, Cristina; Musolino, Vincenzo; Gliozzi, Micaela; Maiuolo, Jessica; Mollace, Rocco; Nucera, Saverio; Maretta, Alessia; Sergi, Domenico; Muscoli, Saverio; Gratteri, Santo; Palma, Ernesto; Bosco, Francesca; Giancotta, Caterina; Muscoli, Carolina; Marino, Fabiola; Aquila, Iolanda; Torella, Daniele; Romeo, Franco; Mollace, Vincenzo

2018-04-12

Doxorubicin (DOXO) is one of the most widely used antineoplastic drugs. Despite its highly beneficial effects against several malignancies, the clinical use of DOXO is often associated to cardiomyopathy that leads to congestive heart failure. Here we investigated the antioxidant and cardioprotective effects of a polyphenol-rich fraction of citrus bergamot (BPF), in DOXO-induced cardiac damage in rats. Moreover, we evaluated the effect of BPF on cardiomyocyte survival and resident endogenous cardiac stem/progenitor cell (eCSC) activation. Adult male Wistar rats were i.p. injected with saline (serving as controls, CTRL, n = 10), BPF (20 mg/kg daily for 14 consecutive days, n = 10), DOXO (6 doses of 2,5 mg/Kg from day 1 to day 14, n = 10), and DOXO + BPF (n = 10). Animals were then sacrificed 7 days later (i.e., at 21 days). DOXO administration reduced cardiac function at 21 days, an adverse effect significantly attenuated in animals receiving DOXO + BPF. No changes were detected in rats receiving just saline or BPF alone. The cardioprotective effect of BPF on DOXO acute toxicity was also associated with a significant antioxidant effect coupled with protective autophagy restoration, and attenuation of cardiomyocyte apoptosis and reactive hypertrophy. Finally, treatment of rats with BPF prevented eCSCs attrition by DOXO which was followed by a limited but significant increase of newly-formed BrdU + cardiomyocytes. In conclusion, BPF reduces DOXO-induced cardiotoxicity by counteracting reactive oxygen species (ROS) overproduction, thereby restoring protective autophagy and attenuating cardiomyocyte apoptosis and pathologic remodeling. This beneficial effects on the early toxicity of DOXO is associated with enhanced CSCs survival and regenerative potential. Overall these data point to a potential clinical role by diet supplementation with polyphenol-rich fraction of citrus bergamot in counteracting antracycline-induced cardiomyopathy. Copyright © 2018. Published by Elsevier Ltd.

In vitro non-enzymatic ribation reduces post-yield strain accommodation in cortical bone.

PubMed

Willett, Thomas L; Sutty, Sibi; Gaspar, Anne; Avery, Nick; Grynpas, Marc

2013-02-01

Non-enzymatic glycation (NEG) and advanced glycation endproducts (AGEs) may contribute to bone fragility in various diseases, ageing, and other conditions by modifying bone collagen and causing degraded mechanical properties. In this study, we sought to further understand how collagen modification in an in vitro non-enzymatic ribation model leads to loss of cortical bone toughness. Previous in vitro studies using non-enzymatic ribation reported loss of ductility in the cortical bone. Increased crosslinking is most commonly blamed for these changes; however, some studies report positive correlations between measures of total collagen crosslinking and work-to-fracture/toughness measurements whilst correlations between general NEG and measures of ductility are often negative. Fifteen bone beam triplets were cut from bovine metatarsi. Each provided one native non-incubated control, one incubated control and one ribated specimen. Incubation involved simulated body fluid±ribose for fourteen days at 37°C. Pentosidine and pyridinoline crosslinks were measured using HPLC. Three-point bending tests quantified mechanical properties. Fracture surfaces were examined using scanning electron microscopy. The effects of ribation on bone collagen molecular stability and intermolecular connectivity were investigated using differential scanning calorimetry and hydrothermal isometric tension testing. Ribation caused increased non-enzymatic collagen modification and pentosidine content (16mmol/mol collagen) and inferior post-yield mechanical behaviour, especially post-yield strain and flexural toughness. Fracture surfaces were smoother with less collagen fibril deformation or tearing than observed in controls. In the ribated group only, pentosidine content and thermomechanical measures of crosslinking were positively correlated with measures of strain accommodation and energy absorption before failure. Non-enzymatic ribation and the resulting modifications reduce cortical bone pseudo-plasticity through a reduced capacity for post-yield strain accommodation. However, the positive correlations we have found suggest that increased crosslinking may not provide a complete explanation for this embrittlement. Copyright © 2012 Elsevier Inc. All rights reserved.

N-gram support vector machines for scalable procedure and diagnosis classification, with applications to clinical free text data from the intensive care unit.

PubMed

Marafino, Ben J; Davies, Jason M; Bardach, Naomi S; Dean, Mitzi L; Dudley, R Adams

2014-01-01

Existing risk adjustment models for intensive care unit (ICU) outcomes rely on manual abstraction of patient-level predictors from medical charts. Developing an automated method for abstracting these data from free text might reduce cost and data collection times. To develop a support vector machine (SVM) classifier capable of identifying a range of procedures and diagnoses in ICU clinical notes for use in risk adjustment. We selected notes from 2001-2008 for 4191 neonatal ICU (NICU) and 2198 adult ICU patients from the MIMIC-II database from the Beth Israel Deaconess Medical Center. Using these notes, we developed an implementation of the SVM classifier to identify procedures (mechanical ventilation and phototherapy in NICU notes) and diagnoses (jaundice in NICU and intracranial hemorrhage (ICH) in adult ICU). On the jaundice classification task, we also compared classifier performance using n-gram features to unigrams with application of a negation algorithm (NegEx). Our classifier accurately identified mechanical ventilation (accuracy=0.982, F1=0.954) and phototherapy use (accuracy=0.940, F1=0.912), as well as jaundice (accuracy=0.898, F1=0.884) and ICH diagnoses (accuracy=0.938, F1=0.943). Including bigram features improved performance on the jaundice (accuracy=0.898 vs 0.865) and ICH (0.938 vs 0.927) tasks, and outperformed NegEx-derived unigram features (accuracy=0.898 vs 0.863) on the jaundice task. Overall, a classifier using n-gram support vectors displayed excellent performance characteristics. The classifier generalizes to diverse patient populations, diagnoses, and procedures. SVM-based classifiers can accurately identify procedure status and diagnoses among ICU patients, and including n-gram features improves performance, compared to existing methods. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.

Lack of Siglec-7 expression identifies a dysfunctional natural killer cell subset associated with liver inflammation and fibrosis in chronic HCV infection.

PubMed

Varchetta, Stefania; Mele, Dalila; Lombardi, Andrea; Oliviero, Barbara; Mantovani, Stefania; Tinelli, Carmine; Spreafico, Marta; Prati, Daniele; Ludovisi, Serena; Ferraioli, Giovanna; Filice, Carlo; Aghemo, Alessio; Lampertico, Pietro; Facchetti, Floriana; Bernuzzi, Francesca; Invernizzi, Pietro; Mondelli, Mario U

2016-12-01

Sialic-acid-binding immunoglobulin-like lectin-7 (Siglec-7) is a natural killer (NK) cell inhibitory receptor associated with NK phenotypic and functional abnormalities in HIV-1 infection. We investigated the significance of NK-expressed and serum soluble Siglec-7 in relation to NK functional ability and parameters of liver necroinflammation and fibrosis in chronic HCV infection. NK-expressed and serum Siglec-7 were evaluated in 130 and 166 HCV-infected individuals by flow cytometry and ELISA, respectively. NK cell degranulation and cytokine secretion were determined by flow cytometry. 65 patients with chronic HBV infection, 84 with chronic biliary disorders and 168 healthy donors served as controls. Expression of Siglec-7 was significantly decreased on NK cells from HCV-infected and HBV-infected patients and, conversely, serum Siglec-7 was significantly increased in these patients compared with controls. The frequency of Siglec-7pos NK cells was significantly higher at baseline in sustained virological responders to pegylated interferon-α/ribavirin treatment than in non-responders. Activating receptor expression was significantly higher in Siglec-7pos NK cells and was associated with increased degranulation and cytokine secretion compared with Siglec-7 neg cells. In chronic HCV infection, there was an inverse correlation between Siglec-7 expression and serum aminotransferases, γ-glutamyl transpeptidase, liver stiffness, aspartate aminotransferase to platelet ratio index and fibrosis-4 scores, and a positive correlation between serum Siglec-7 and the same clinical parameters, including histological staging. These findings identify Siglec-7 neg NK cells as a dysfunctional subpopulation associated with severe liver disease in chronic HCV infection. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/.

Effect of Age on Energy Requirement for Maintenance and Growth of Dorper and Hu Crossbred F1 Ewes Weighing 20 to 50 kg

PubMed Central

Nie, H. T.; Wan, Y. J.; You, J. H.; Wang, Z. Y.; Lan, S.; Fan, Y. X.; Wang, F.

2015-01-01

This research aimed to define the energy requirement of Dorper and Hu Hybrid F1 ewes 20 to 50 kg of body weight, furthermore to study energy requirement changes with age and evaluate the effect of age on energy requirement parameters. In comparative slaughter trial, thirty animals were divided into three dry matter intake treatments (ad libitum, n = 18; low restricted, n = 6; high restricted, n = 6), and were all slaughtered as baseline, intermediate, and final slaughter groups, to calculate body chemical components and energy retained. In digestibility trial, twelve ewes were housed in individual metabolic cages and randomly assigned to three feeding treatments in accordance with the design of a comparative slaughter trial, to evaluate dietary energetic values at different feed intake levels. The combined data indicated that, with increasing age, the net energy requirement for maintenance (NEm) decreased from 260.62±13.21 to 250.61±11.79 kJ/kg0.75 of shrunk body weight (SBW)/d, and metabolizable energy requirement for maintenance (MEm) decreased from 401.99±20.31 to 371.23±17.47 kJ/kg0.75 of SBW/d. Partial efficiency of ME utilization for maintenance (km, 0.65 vs 0.68) and growth (kg, 0.42 vs 0.41) did not differ (p>0.05) due to age; At the similar condition of average daily gain, net energy requirements for growth (NEg) and metabolizable energy requirements for growth (MEg) for ewes during late fattening period were 23% and 25% greater than corresponding values of ewes during early fattening period. In conclusion, the effect of age upon energy requirement parameters in the present study were similar in tendency with previous recommendations, values of energy requirement for growth (NEg and MEg) for Dorper and Hu crossbred female lambs ranged between the NRC (2007) recommendation for early and later maturating growing sheep. PMID:26104522

CXCL16-positive dendritic cells enhance invariant natural killer T cell-dependent IFNγ production and tumor control

PubMed Central

Veinotte, Linnea; Gebremeskel, Simon; Johnston, Brent

2016-01-01

ABSTRACT Crosstalk interactions between dendritic cells (DCs) and invariant natural killer T (iNKT) cells are important in regulating antitumor responses elicited by glycolipid antigens. iNKT cells constitutively express the chemokine receptor CXCR6, while cytokine-activated DCs upregulate the transmembrane chemokine ligand, CXCL16. This study examined the co-stimulatory role of CXCR6/CXCL16 interactions in glycolipid-dependent iNKT cell activation and tumor control. Spleen and liver DCs in wild-type mice, but not iNKT cell deficient (Jα18−/−) mice, transiently upregulated surface CXCL16 following in vivo administration of the glycolipid antigen α-galactosylceramide. Recombinant CXCL16 did not directly induce iNKT cell activation in vitro but enhanced interferon (IFN)-γ production when mouse or human iNKT cells were stimulated with plate-bound anti-CD3. Compared with glycolipid-loaded CXCL16neg DCs, CXCL16hi DCs induced higher levels of IFNγ production in iNKT cell cultures and following adoptive transfer in vivo. The number of IFNγ+ iNKT cells and expansion of T-bet+ iNKT cells were reduced in vivo when CXCL16−/− DCs were used to activate iNKT cells. Enhanced IFNγ production in vivo was not dependent on CXCR6 expression on natural killer (NK) cells. Adoptive transfer of glycolipid-loaded CXCL16hi DCs provided superior protection against tumor metastasis compared to CXCL16neg DC transfers. Similarly, wild-type DCs provided superior protection against metastasis compared with CXCL16−/− DCs. These experiments implicate an important role for CXCR6/CXCL16 interactions in regulating iNKT cell IFNγ production and tumor control. The selective use of CXCL16hi DCs in adoptive transfer immunotherapies may prove useful for enhancing T helper (Th) type 1 responses and clinical outcomes in cancer patients. PMID:27471636

Peripheral blood stem cell mobilization: the CXCR2 ligand GRObeta rapidly mobilizes hematopoietic stem cells with enhanced engraftment properties.

PubMed

Pelus, Louis M; Fukuda, Seiji

2006-08-01

Chemokines direct the movement of leukocytes, including hematopoietic stem and progenitor cells, and can mobilize hematopoietic cells from marrow to peripheral blood where they can be used for transplantation. In this review, we will discuss the stem cell mobilizing activities and mechanisms of action of GRObeta, a CXC chemokine ligand for the CXCR2 receptor. GRObeta rapidly mobilizes short- and long-term repopulating cells in mice and/or monkeys and synergistically enhances mobilization responses when combined with the widely used clinical mobilizer, granulocyte colony-stimulating factor (G-CSF). The hematopoietic graft mobilized by GRObeta contains significantly more CD34(neg), Sca-1+, c-kit+, lineage(neg) (SKL) cells than the graft mobilized by G-CSF. In mice, stem cells mobilized by GRObeta demonstrate a competitive advantage upon long-term repopulation analysis and restore neutrophil and platelet counts significantly faster than cells mobilized by G-CSF. Even greater advantage in repopulation and restoration of hematopoiesis are observed with stem cells mobilized by the combination of GRObeta and G-CSF. GRObeta-mobilized SKL cells demonstrate enhanced adherence to vascular cell adhesion molecule-1 and VCAM(pos) endothelial cells and home more efficiently to bone marrow in vivo. The marrow homing ability of GRObeta-mobilized cells is less dependent on the CXCR4/SDF-1 axis than cells mobilized by G-CSF. The mechanism of mobilization by GRObeta requires active matrix metalloproteinase-9 (MMP-9), which results from release of pro-MMP-9 from peripheral blood, and marrow neutrophils, which alters the stoichiometry between pro-MMP-9 and its inhibitor tissue inhibitor of metalloproteinase-1, resulting in MMP-9 activation. The efficacy and rapid action of GRObeta and lack of proinflammatory activity make it an attractive agent to supplement mobilization by G-CSF. In addition, GRObeta may also have clinical mobilizing efficacy on its own, reducing the overall time and costs associated with peripheral blood stem cell transplantation.

Attenuation of cardiac fibrosis by pirfenidone and amiloride in DOCA-salt hypertensive rats

PubMed Central

Mirkovic, Stevo; Seymour, Anne-Marie L; Fenning, Andrew; Strachan, Anna; Margolin, Solomon B; Taylor, Stephen M; Brown, Lindsay

2002-01-01

This study has administered pirfenidone (5-methyl-1-phenyl-2-[1H]-pyridone) or amiloride to attenuate the remodelling and associated functional changes, especially an increased cardiac stiffness, in DOCA-salt hypertensive rats. In control rats, the elimination half-life of pirfenidone following a single intravenous dose of 200 mg kg−1 was 37 min while oral bioavailability at this dose was 25.7%. Plasma pirfenidone concentrations in control rats averaged 1.9±0.1 μg ml−1 over 24 h after 14 days' administration as a 0.4% mixture in food. Pirfenidone (approximately 250 – 300 mg kg−1 day−1 as 0.4% in food) and amiloride (1 mg kg−1 day−1 sc) were administered for 2 weeks starting 2 weeks post-surgery. Pirfenidone but not amiloride attenuated ventricular hypertrophy (2.69±0.09, UNX 2.01±0.05. DOCA-salt 3.11±0.09 mg kg−1 body wt) without lowering systolic blood pressure. Collagen deposition was significantly increased in the interstitium after 2 weeks and further increased with scarring of the left ventricle after 4 weeks; pirfenidone and amiloride reversed the increases and prevented further increases. This accumulation of collagen was accompanied by an increase in diastolic stiffness constant; both amiloride and pirfenidone reversed this increase. Noradrenaline potency (positive chronotropy) was decreased in right atria (neg log EC50: control 6.92±0.06; DOCA-salt 6.64±0.08); pirfenidone but not amiloride reversed this change. Noradrenaline was a more potent vasoconstrictor in thoracic aortic rings (neg log EC50: control 6.91±0.10; DOCA-salt 7.90±0.07); pirfenidone treatment did not change noradrenaline potency. Thus, pirfenidone and amiloride reverse and prevent cardiac remodelling and the increased cardiac stiffness without reversing the increased vascular responses to noradrenaline. PMID:11861324

Autoimmune hemolytic anemia in a patient with Malaria

PubMed Central

Sonani, Rajesh; Bhatnagar, Nidhi; Maitrey, Gajjar

2013-01-01

Autoimmune Hemolytic Anemia (AIHA), a very infrequent condition which represents a group of disorders in which presence of autoantibodies directed against self-antigens leads to shortened red cell survival. Till date, a very few cases of AIHA in Malaria patients are reported worldwide but still AIHA should be considered a relatively rare cause of anemia in malaria. A 20 year male presented with intermittent fever since seven days and yellowish discoloration of urine and sclera since 5 days. He was transfused three units of blood at a private clinic before one month. On examination, pallor, icterus and spelnomegaly were present. Hemoglobin (Hb) was 3.2 gm% and peripheral smear revealed ring forms of both Plasmodium vivax and Plasmodium falciparum. Serum LDH and Serum billirubin (Indirect and Direct) were high. This patient’s blood group was B +ve with positive autocontrol. Indirect Antiglobulin Test (IAT), antibody screening and antibody identification were pan-positive with reaction strength of +4 against each cell. Direct Antiglobulin Test was +4 positive anti IgG and negative with anti C3. He was treated with Artesunate and methylprednisone. Least incompatible, saline washed O Neg and B neg red cells were transfused on the 2nd day of starting treatment. Hb was raised to 6.1 gm% on 4th day. Patient was discharged on 9th day with Hb 7.0 gm% with oral tapering dose of steroids. In the above case, patient was suffering from high grade malarial parasitemia with co-existing autoimmune RBC destruction by IgG auto-antibodies which led to sudden drop in Hb and rise in serum LDH and indirect billirubin. Least incompatible packed red cells along with antimalarials and steroids led to clinical improvement. So far, one case report each from India, Korea, Canada and Germany and one case series report of three cases from India have been reported. Under-reporting or rarity of this phenomenon may be accountable for this. PMID:24014948

Autoimmune hemolytic anemia in a patient with Malaria.

PubMed

Sonani, Rajesh; Bhatnagar, Nidhi; Maitrey, Gajjar

2013-07-01

Autoimmune Hemolytic Anemia (AIHA), a very infrequent condition which represents a group of disorders in which presence of autoantibodies directed against self-antigens leads to shortened red cell survival. Till date, a very few cases of AIHA in Malaria patients are reported worldwide but still AIHA should be considered a relatively rare cause of anemia in malaria. A 20 year male presented with intermittent fever since seven days and yellowish discoloration of urine and sclera since 5 days. He was transfused three units of blood at a private clinic before one month. On examination, pallor, icterus and spelnomegaly were present. Hemoglobin (Hb) was 3.2 gm% and peripheral smear revealed ring forms of both Plasmodium vivax and Plasmodium falciparum. Serum LDH and Serum billirubin (Indirect and Direct) were high. This patient's blood group was B +ve with positive autocontrol. Indirect Antiglobulin Test (IAT), antibody screening and antibody identification were pan-positive with reaction strength of +4 against each cell. Direct Antiglobulin Test was +4 positive anti IgG and negative with anti C3. He was treated with Artesunate and methylprednisone. Least incompatible, saline washed O Neg and B neg red cells were transfused on the 2(nd) day of starting treatment. Hb was raised to 6.1 gm% on 4(th) day. Patient was discharged on 9th day with Hb 7.0 gm% with oral tapering dose of steroids. In the above case, patient was suffering from high grade malarial parasitemia with co-existing autoimmune RBC destruction by IgG auto-antibodies which led to sudden drop in Hb and rise in serum LDH and indirect billirubin. Least incompatible packed red cells along with antimalarials and steroids led to clinical improvement. So far, one case report each from India, Korea, Canada and Germany and one case series report of three cases from India have been reported. Under-reporting or rarity of this phenomenon may be accountable for this.

HLA Class I Alleles Associated with Mortality in Thai Military Recruits with HIV-1 CRF01_AE Infection

PubMed Central

Bosch, Ronald J.; Rangsin, Ram; Chuenchitra, Thippawan; Sirisopana, Narongrid; Kim, Jerome H.; Robb, Merlin L.; Vejbaesya, Sasijit; Paris, Robert M.; Nelson, Kenrad E.

2016-01-01

Abstract In HIV-1-infected patients, variation at the HLA class I locus is associated with disease progression, but few studies have assessed the influence of HLA alleles on HIV-1 CRF01_AE infection, which is dominant in Thailand. We hypothesized that alleles predicted to confer more effective immune responses, such as HLA-B*46, would protect against disease progression. HLA typing was performed on HIV-1 incident cases surviving until 1998–1999 and HIV-1-negative matched controls from Thai army cohorts enrolled between 1991 and 1995. We assessed associations between class I alleles and disease progression subsequent to HLA typing. Ninety-nine HIV-1-incident cases were followed for a median of 3.7 years after HLA typing; during this time, 58 participants died. Two alleles were associated with mortality: HLA B*51 was protective (3-year survival B*51pos vs. B*51neg: 75% vs. 52%; p = 0.034) whereas Cw*04 was deleterious (3-year survival Cw*04pos vs. Cw*04neg: 39% vs. 60%; p = 0.027). HLA-B*46 was not associated with disease progression. Alleles present at different frequencies in HIV-1-incident compared with HIV-1-negative men included HLA-A*02:03, B*35, B*15, and C*08. 1. In conclusion in this Thai army cohort, HLA-B*51 was associated with lower mortality, confirming that this allele, which is protective in clade B HIV-1 infection, has a similar effect on HIV CRF01_AE infection. The deleterious effect of HLA-Cw*04 must be interpreted with caution because it may be in linkage disequilibrium with disease-susceptible HLA-B alleles. We did not find that HLA-B*46 was protective. These findings may inform vaccine development for areas of the world in which HIV-1 CRF01_AE infection is prevalent. PMID:26383907

Development and test-retest reliability of an extended version of the Nordic Musculoskeletal Questionnaire (NMQ-E): a screening instrument for musculoskeletal pain.

PubMed

Dawson, Anna P; Steele, Emily J; Hodges, Paul W; Stewart, Simon

2009-05-01

The Nordic Musculoskeletal Questionnaire (NMQ) quantifies musculoskeletal pain and activity prevention in 9 body regions. The purpose of this study was to develop an extended NMQ (NMQ-E) to collect greater information regarding musculoskeletal pain, examine test-retest reliability and the reproducibility of alternate administration methods. Reliability was examined using observed proportion of agreement for all (P(o)), positive (P(pos)) and negative (P(neg)) responses, kappa (kappa), proportion of maximum kappa achieved (kappa/kappa(max)), intra-class correlation coefficient (ICC) and standard error of measurement (SEM). The NMQ-E was self-administered by 59 Bachelor of Nursing students at a 24-h interval with mean P(o) = 0.88-0.98 and kappa/kappa(max) = 0.71-0.96 for 10 dichotomous questions and mean ICC((2,1)) = 0.97 and SEM = 1.05 years for the age at symptom onset question. The NMQ-E was completed via self and interview administration by 31 student nurses at a 0.97 +/- 1.14 day interval with mean P(o) = 0.92-0.98 and kappa/kappa(max) = 0.76-1.00 for binary questions and mean ICC((2,1)) = 0.90 and SEM = 1.51 years for age at symptom onset data. In both sub-studies, mean P(pos) was lower than mean P(neg) and low prevalence reduced kappa in many instances. The NMQ-E collects reliable information regarding the onset, prevalence, and consequences of musculoskeletal pain and can be administered by self-completion and personal interview. This study presents an NMQ-E that collects reliable information regarding the onset, prevalence, and consequences of musculoskeletal pain in 9 body regions. The NMQ-E can be utilized in descriptive studies or longitudinal studies of disease outcome and can be administered via self-completion and personal interview.

Medical simulation technology: educational overview, industry leaders, and what's missing.

PubMed

Spooner, Nicholas; Hurst, Stephen; Khadra, Mohamed

2012-01-01

Modern medical simulation technology (MST) debuted in 1960 with the development of Resusci Annie (Laerdal 2007), which assisted students in the acquisition of proper ventilation and compression techniques used during basic life support. Following a steady stream of subsequent technological advances and innovations, MST manufacturers are now able to offer training aids capable of facilitating innovative learning in such diverse areas as human patient simulators, simulated clinical environments, virtual procedure stations, virtual medical environments, electronic tutors, and performance recording. The authors list a number of the most popular MSTs presently available while citing evaluative efforts undertaken to date regarding the efficacy of MST to the medical profession. They conclude by proposing a variety of simulation innovations of prospective interest to both medical and technology personnel while offering healthcare administrators a series of recommended considerations when planning to integrate MST into existing medical systems.

KSC-98pc1579

NASA Image and Video Library

1998-11-08

At the Skid Strip at Cape Canaveral Air Station, STS-95 Payload Specialist John H. Glenn Jr., a senator from Ohio and one of the original seven Project Mercury astronauts, poses with his wife Annie before their return flight to the Johnson Space Center in Houston, Texas. The STS-95 mission ended with landing at Kennedy Space Center's Shuttle Landing Facility at 12:04 p.m. EST on Nov. 7. The STS-95 crew also includes Mission Commander Curtis L. Brown Jr.; Pilot Steven W. Lindsey; Mission Specialist Scott E. Parazynski; Mission Specialist Stephen K. Robinson; Mission Specialist Pedro Duque, with the European Space Agency (ESA); and Payload Specialist Chiaki Mukai, with the National Space Development Agency of Japan (NASDA). The mission included research payloads such as the Spartan-201 solar-observing deployable spacecraft, the Hubble Space Telescope Orbital Systems Test Platform, the International Extreme Ultraviolet Hitchhiker, as well as a SPACEHAB single module with experiments on space flight and the aging process

17. Copy of a post card showing the Lavelle School ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

17. Copy of a post card showing the Lavelle School and its first graduating class, c. 1890. The people are identified as: (1st row L to R) Charles Walker, Raymond Hornberger, Kenneth Keller, Dan Walker, Gilbert Lenker, and John Wetzel (from Locustdale); (2nd row L to R) Tim Snyder, Art Hornberger, John Wetzel (from Lavelle), ? Keller, Bright Enterline, Emerson Keller, Art Krah, and Leroy Henninger; (3rd row L to R) Hannah Wetzel, Vernie Hepler, Nona Snyder, Alice Dillman, Edith (Krah) Wolfgang, and Florence (Leach) Paul; (4th row L to R) Elva Paul, Vernie Wolfgang, Marie Enterline Walker, and Annie Hunter; (5th row L to R) Edna Walker, Martha Hornberger (Teacher), and Emma Dillman. (Copy of photo reproduced with permission from the Ashland Public ... - Lavelle School, Township Road 905, Main Street, Village of Lavelle, Lavelle, Schuylkill County, PA

IL TRAPIANTO ORTOTOPICO DEL FEGATO

PubMed Central

STARZL, THOMAS E.

2010-01-01

E’ormai noto che esiste la possibilità rivoluzionaria di utilizzare il fegato per il trattamento della stadio terminale delle epatopatie. Nel gennaio 1980 si celebra il decimo anna di sopravvivenza con fegato trapiantato (la più lunga della letteratura) di un paziente da noi trattato. Si tratta di uno dei 12 malati sottoposti a trapianto e seguiti per più di 5 anni. La nota positiva di questa tipo di trattamento è rappresentata dall’eccellente tenore di vita che i pazienti conducono e dalla riabilitazione sociale e professionale. La nota negativa è data, invece, dal fatto che i buoni risultati non vengono raggiunti con regolarità e non possono essere previsti con esattezza. In questa breve rassegna considereremo la esperienza da noi fatta presso l’Università di Denver nel Colorado, mettendo in risalto le cause dell’elevata mortalità precoce e le prospettive future di questa mezzo terapeutico. PMID:21572898

Performance of 12Ah aerospace nickel-cadmium cells of design variable groups

NASA Astrophysics Data System (ADS)

Vasanth, K. L.

1985-12-01

The design variable program of NASA is a systematic approach to evaluate the performance of 12Ah aerospace nickel-cadmium cells of 9 important cell designs. These cells were life cycled in a Low-Earth Orbit (LEO) regime for 3 to 4 years. Representative cells taken from the design variable groups after different cycling periods have been examined. The results show that: (1) positive swelling and carbonate content in the electrolyte increases as a function of the number of cycles, (2) electrolyte distribution follows the order NEG greater than POS greater than SEP, 3) control and no PQ groups outperformed the rest of the groups and (4) the polypropylene group shows very heavy cadmium migration and poor performance.

Performance of 12Ah aerospace nickel-cadmium cells of design variable groups

NASA Technical Reports Server (NTRS)

Vasanth, K. L.

1985-01-01

The design variable program of NASA is a systematic approach to evaluate the performance of 12Ah aerospace nickel-cadmium cells of 9 important cell designs. These cells were life cycled in a Low-Earth Orbit (LEO) regime for 3 to 4 years. Representative cells taken from the design variable groups after different cycling periods have been examined. The results show that: (1) positive swelling and carbonate content in the electrolyte increases as a function of the number of cycles, (2) electrolyte distribution follows the order NEG greater than POS greater than SEP, 3) control and no PQ groups outperformed the rest of the groups and (4) the polypropylene group shows very heavy cadmium migration and poor performance.

Cosmic Ink: Fragments from the Past on Journal Pages

NASA Astrophysics Data System (ADS)

Mandrino, A.; Gargano, M.; Gasperini, A.

2015-04-01

This contribution describes an editorial project started in 2012 to enhance the cultural heritage of the Italian observatories. It includes a regular column Cieli di inchiostro (Cosmic ink) devoted to promoting the astronomical historical archives and published in the Giornale di astronomia, a journal of the Società Astronomica Italiana. In every issue of the journal, a significant historical artifact is presented and described. This can be a letter, a diary page, a photograph, a map, a drawing, or another type of item pulled out of the archival folders to bring its history to light. The column is intended to invite historians, amateurs, and students to search and use the documents kept in the archives of the observatories.

KSC-98pc1775

NASA Image and Video Library

1998-12-03

KENNEDY SPACE CENTER, FLA. -- Participants pose for a photo at the Space Station Processing Facility ceremony transferring the "Leonardo" Multipurpose Logistics Module (MPLM) from the Italian Space Agency, Agenzia Spaziale Italiana (ASI), to NASA. From left, they are astronaut Jim Voss, ASI President Sergio De Julio, European Space Agency astronaut Umberto Guidoni of Italy, NASA Administrator Daniel S. Goldin and European Space Agency astronaut Christer Fuglesang of Sweden. The MPLM, a reusable logistics carrier, will be the primary delivery system used to resupply and return International Space Station cargo requiring a pressurized environment. Leonardo is the first of three MPLM carriers for the International Space Station. It is scheduled to be launched on Space Shuttle Mission STS-100, targeted for April 2000

KSC-98pc1774

NASA Image and Video Library

1998-12-03

KENNEDY SPACE CENTER, FLA. -- Participants pose for a photo at the Space Station Processing Facility ceremony transferring the "Leonardo" Multipurpose Logistics Module (MPLM) from the Italian Space Agency, Agenzia Spaziale Italiana (ASI), to NASA. From left, they are astronaut Jim Voss, ASI President Sergio De Julio, European Space Agency astronaut Umberto Guidoni of Italy, NASA Administrator Daniel S. Goldin and European Space Agency astronaut Christer Fuglesang of Sweden. The MPLM, a reusable logistics carrier, will be the primary delivery system used to resupply and return International Space Station cargo requiring a pressurized environment. Leonardo is the first of three MPLM carriers for the International Space Station. It is scheduled to be launched on Space Shuttle Mission STS-100, targeted for April 2000

The role of dosimetry audit in lung SBRT multi-centre clinical trials.

PubMed

Clark, Catharine H; Hurkmans, Coen W; Kry, Stephen F

2017-12-01

Stereotactic Body Radiotherapy (SBRT) in the lung is a challenging technique which requires high quality clinical trials to answer the un-resolved clinical questions. Quality assurance of these clinical trials not only ensures the safety of the treatment of the participating patients but also minimises the variation in treatment, thus allowing the lowest number of patient treatments to answer the trial question. This review addresses the role of dosimetry audits in the quality assurance process and considers what can be done to ensure the highest accuracy of dose calculation and delivery and it's assessment in multi-centre trials. Copyright © 2017 Associazione Italiana di Fisica Medica. Published by Elsevier Ltd. All rights reserved.

Work continues on Leonardo, the Multi-Purpose Logistics Module, in the Space Station Processing Faci

NASA Technical Reports Server (NTRS)

1999-01-01

Workers in the Space Station Processing Facility work on Leonardo, the Multipurpose Logistics Module (MPLM) built by the Agenzia Spaziale Italiana (ASI). The MPLM, a reusable logistics carrier, will be the primary delivery system used to resupply and return International Space Station cargo requiring a pressurized environment. Leonardo is the first of three MPLM carriers for the International Space Station. It is scheduled to be launched on Space Shuttle Mission STS-102, targeted for June 2000. Leonardo shares space in the SSPF with the Shuttle Radar Topography Mission (SRTM), targeted for launch in September 1999, and Destiny, the U.S. Lab module, targeted for mission STS-98 in late April 2000.

Hard X-ray index of refraction tomography of a whole rabbit knee joint: A feasibility study.

PubMed

Gasilov, S; Mittone, A; Horng, A; Geith, T; Bravin, A; Baumbach, T; Coan, P

2016-12-01

We report results of the computed tomography reconstruction of the index of refraction in a whole rabbit knee joint examined at the photon energy of 51keV. Refraction based images make it possible to delineate the bone, cartilage, and soft tissues without adjusting the contrast window width and level. Density variations, which are related to tissue composition and are not visible in absorption X-ray images, are detected in the obtained refraction based images. We discuss why refraction-based images provide better detectability of low contrast features than absorption images. Copyright © 2016 Associazione Italiana di Fisica Medica. Published by Elsevier Ltd. All rights reserved.

Coeliac disease: the histology report.

PubMed

Villanacci, Vincenzo; Ceppa, Paola; Tavani, Enrico; Vindigni, Carla; Volta, Umberto

2011-03-01

To this day intestinal biopsy is justly considered the "gold standard" for the diagnosis of coeliac disease (CD). The aim of the authors in setting up these guidelines was to assist pathologists in formulating a more precise morphological evaluation of a duodenal biopsy in the light of clinical and laboratory data, to prepare histological samples with correctly oriented biopsies and in the differential diagnosis with other pathological entities and complications of the disease. A further intention was to promote the conviction for the need of a close collaborative relationship between different specialists namely the concept of a "multidisciplinary team". Copyright © 2011 Editrice Gastroenterologica Italiana S.r.l. Published by Elsevier Ltd.. All rights reserved.

The European Federation of Organisations for Medical Physics Policy Statement No 14: the role of the Medical Physicist in the management of safety within the magnetic resonance imaging environment: EFOMP recommendations.

PubMed

Hand, J; Bosmans, H; Caruana, C; Keevil, S; Norris, D G; Padovani, R; Speck, O

2013-03-01

This European Federation of Organisations for Medical Physics (EFOMP) Policy Statement outlines the way in which a Safety Management System can be developed for MRI units. The Policy Statement can help eliminate or at least minimize accidents or incidents in the magnetic resonance environment and is recommended as a step towards harmonisation of safety of workers, patients, and the general public regarding the use of magnetic resonance imaging systems in diagnostic and interventional procedures. Copyright © 2012 Associazione Italiana di Fisica Medica. Published by Elsevier Ltd. All rights reserved.

Early management of acute pancreatitis: A review of the best evidence.

PubMed

Stigliano, Serena; Sternby, Hanna; de Madaria, Enrique; Capurso, Gabriele; Petrov, Maxim S

2017-06-01

In the 20th century early management of acute pancreatitis often included surgical intervention, despite overwhelming mortality. The emergence of high-quality evidence (randomized controlled trials and meta-analyses) over the past two decades has notably shifted the treatment paradigm towards predominantly non-surgical management early in the course of acute pancreatitis. The present evidence-based review focuses on contemporary aspects of early management (which include analgesia, fluid resuscitation, antibiotics, nutrition, and endoscopic retrograde cholangiopancreatography) with a view to providing clear and succinct guidelines on early management of patients with acute pancreatitis in 2017 and beyond. Copyright © 2017 Editrice Gastroenterologica Italiana S.r.l. Published by Elsevier Ltd. All rights reserved.

Kikuchi-Fujimoto disease: a clinicopathologic update.

PubMed

Pepe, F; Disma, S; Teodoro, C; Pepe, P; Magro, G

2016-09-01

Kikuchi-Fujmoto disease (KFD), also known as "histiocytic necrotizing lymphadenitis", is a rare lymphadenitis of unknown origin, but with an excellent prognosis. It is more common in Asia, but isolated cases are also reported in America, Africa and Europe. The disease can have an acute or subacute course, usually develops in 1 to 3 weeks, with spontaneous resolution in 1-4 months. The main clinical sign is cervical lymphadenopathy, especially in the posterior cervical triangle with bulky and painful lymph nodes, usually affecting only one side; rare cases of generalized lymphadenopathy can be seen. This common clinical presentation can also be accompanied by nausea, vomiting, weight loss, weakness, headache and arthralgia. An extranodal extension of the disease, including involvement of skin, eye, and bone marrow localizations, has been rarely described. Most patients have leukopenia or neutropenia with a relative leukocytosis. At an ultrasound exploration of the affected lymph nodes, a hypoechoic aspect can be seen, with an external, thick and irregular hyperechoic ring. As there are no specific tests for KFD, the final diagnosis is histologically-based from lymph node excisional biopsy. Histological examination shows paracortical foci of coagulative necrosis containing karyorrhectic debris, which are surrounded by numerous CD68+/myeloperoxidase (MPO)+ histiocytes, CD68+/CD123+ plasmacytoid dendritic cells, and a minority of small- to large-sized CD8+lymphocytes and immunoblasts. Differential diagnosis mainly includes systemic lupus erithematous (SLE)-related lymphadenopathy and large cell lymphoma. The histological absence of neutrophils, plasmacells, as well as hematoxylin bodies, is a feature which argues against the diagnosis of SLE. In addition, the absence of auto-antibodies and anti-nuclear antibodies is useful in ruling out an autoimmune disorder. Early diagnosis of KFD is crucial to prevent the patients undergo extensive investigations related to suspected malignant lymphomas or other diseases. © Copyright Società Italiana di Anatomia Patologica e Citopatologia Diagnostica, Divisione Italiana della International Academy of Pathology.

Building blocks of the GIPU, Italian Group of Ultrastructural Pathology.

PubMed

Papa, V; Costa, R; Cenacchi, G

2016-06-01

The Italian Group of Ultrastructural Pathology, GIPU, is a scientific organization committed to promote the art and science of Electron Microscopy (EM) in the pathology field in Italy, sharing its professional work with a public audience. The history of the GIPU goes back to 1990s when a founder group set up the Italian Group of Ultrastructural Diagnostic (GIDU) in Milan. The central focus of annual meetings was on EM, transmission and scanning one, about interesting cases in which it was instrumental in diagnosis. In the 1990s, ultrastructure was still the gold standard for cell/tissue morphology, biology, biochemistry, diagnostic pathology, and played an important role in tailored medicine. So, especially transmission EM, could play a critical role in the diagnosis of various diseases as in human as in animals. Best topics of the annual scientific meetings of the group were kidney, muscle, heart, and liver pathology, infertility, neuropathology, respiratory diseases, skin diseases, storage diseases, tumor pathology, infectious diseases, parasitology, veterinary pathology and more. Nowadays, EM is a method whose importance for diagnosis and pathology is well established: it is still essential in several pathologies, helpful in others, and welcome implemented in eclectic research pathology. Omission of EM likely makes the studies suboptimal and wasteful. So, from 2007 the name of the group has been changed to the Italian Group of Ultrastructural Pathology (GIPU) to favor broader applications of EM also to pathology research field. During last decades, GIDU/GIPU has interconnected with international (Society for Ultrastructural Pathology) and european (European Society of Pathology and Joint Meeting with the European Electron Microscopy Working Group) scientific society, according its statute. By 1991, GIPU has had 40 members: membership in this Group is still open and welcome to all pathologists, PhD, electron microscopy technologists, pathology trainees, and researchers interested in pathology and electron microscopy. © Copyright Società Italiana di Anatomia Patologica e Citopatologia Diagnostica, Divisione Italiana della International Academy of Pathology.

Rituximab, bendamustine, and low-dose cytarabine as induction therapy in elderly patients with mantle cell lymphoma: a multicentre, phase 2 trial from Fondazione Italiana Linfomi.

PubMed

Visco, Carlo; Chiappella, Annalisa; Nassi, Luca; Patti, Caterina; Ferrero, Simone; Barbero, Daniela; Evangelista, Andrea; Spina, Michele; Molinari, Annalia; Rigacci, Luigi; Tani, Monica; Rocco, Alice Di; Pinotti, Graziella; Fabbri, Alberto; Zambello, Renato; Finotto, Silvia; Gotti, Manuel; Carella, Angelo M; Salvi, Flavia; Pileri, Stefano A; Ladetto, Marco; Ciccone, Giovannino; Gaidano, Gianluca; Ruggeri, Marco; Martelli, Maurizio; Vitolo, Umberto

2017-01-01

The combination of rituximab, bendamustine, and cytarabine (R-BAC) was highly active in a pilot trial of mantle cell lymphoma, but its use was restricted by high haematological toxicity. We aimed to assess the efficacy and safety of an R-BAC regimen with low-dose cytarabine (RBAC500). In this multicentre, phase 2 trial, we recruited previously untreated patients with an established histological diagnosis of mantle cell lymphoma from 29 Fondazione Italiana Linfomi centres in Italy. Patients had to be older than 65 years and fit according to the comprehensive geriatric assessment, or aged 60-65 years if they were ineligible for high-dose chemotherapy plus autologous stem-cell transplantation and were fit or unfit. All patients received RBAC500 (rituximab 375 mg/m 2 on day 1, bendamustine 70 mg/m 2 on days 2 and 3, and cytarabine 500 mg/m 2 on days 2-4; all administered intravenously) every 4 weeks for up to six cycles. Primary endpoints were the proportion of patients achieving complete response at the end of treatment and toxicity, defined as the occurrence of any of the stop treatment criteria or of any episode of relevant toxicity. All patients who started at least one cycle of RBAC500 were included in the primary and safety analyses. Using efficacy and toxicity as a composite primary endpoint, we considered the final conclusion positive if more than 28 of 57 patients achieve complete response and fewer than 18 of 57 patients report toxicities. This study is registered with EudraCT, number 2011-005739-23, and ClinicalTrials.gov, number NCT01662050, and is completed. Between May 2, 2012, and Feb 25, 2014, we enrolled 57 patients (median age 71 years, IQR 67-75). 54 (95%) patients received at least four RBAC500 cycles (three discontinued because of toxicity), and 38 (67%) completed six cycles. Two (4%) had disease progression (one after the fourth cycle and one after the sixth cycle). All 52 (91%, lower limit of one-sided 95% CI 85%) remaining patients achieved complete response at the end of treatment. 23 (40%, upper limit of one-sided 95% CI 53%) of 57 patients had at least one episode of relevant toxicity. The most frequent grade 3-4 haematological toxicities were neutropenia (149 [49%] of 304 cycles) and thrombocytopenia (158 [52%]). Most treatment-related non-haematological adverse events were of grade 1-2, with the most frequent ones being fatigue (14 [25%] patients), nausea or vomiting (12 [21%]), and infusion-related reactions or tumour lysis syndrome (12 [21%]). 41 (72%) patients required a dose reduction. 12 patients died during the study, but no deaths were related to treatment. RBAC500 is an effective treatment for elderly patients with mantle cell lymphoma and, despite not meeting our prespecified safety boundary, haematological toxicity was manageable with appropriate supportive care and dose reduction. Since maintenance therapy is not required, RBAC500 could be considered an option and should be studied in phase 3 trials. Fondazione Italiana Linfomi and Mundipharma. Copyright © 2017 Elsevier Ltd. All rights reserved.

IMPEDANCE MEASUREMENT OF VACUUM CHAMBER COMPONENTS FOR THE ADVANCE PHOTON SOURCE (APS) UPGRADE

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sangroula, M.; Lindberg, R.; Lill, R.

2017-06-16

The proposed Advance Photon Source Upgrade (APS-U) employs a multi-bend achromat (MBA) lattice to increase the photon brightness by two to three orders of magnitude. One of the main design challenges of the upgrade is to minimize rf heating and collective instabilities associated with the impedance of small-aperture vacuum components. As part of this effort, my research focuses on impedance measurement and simulation of various MBA vacuum components. Here, we present the summary of the impedance contributions for the APS-U and describe our planned impedance measurement technique, including some measurement results for the non-evaporative getter (NEG)-coated copper chamber and simulationmore » results for other critical components using a novel Goubau line (G-line) set up.« less

Impedance measurement of vacuum chamber components for the Advance Photon Source(APS) Upgrade

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sangroula, M.; Lindberg, R.; Lill, R.

2017-01-01

The proposed Advance Photon Source Upgrade (APS-U) employs a multi-bend achromat (MBA) lattice to increase the photon brightness by two to three orders of magnitude. One of the main design challenges of the upgrade is to minimize rf heating and collective instabilities associated with the impedance of small-aperture vacuum components. As part of this effort, my research focuses on impedance measurement and simulation of various MBA vacuum components. Here, we present the summary of the impedance contributions for the APS-U and describe our planned impedance measurement technique, including some measurement results for the non-evaporative getter (NEG)-coated copper chamber and simulationmore » results for other critical components using a novel Goubau line (G-line) set up.« less

Urea and impairment of the Gut-Kidney axis in Chronic Kidney Disease.

PubMed

Di Iorio, Biagio Raffaele; Marzocco, Stefania; Nardone, Luca; Sirico, Marilisa; De Simone, Emanuele; Di Natale, Gabriella; Di Micco, Lucia

2017-12-05

Gut microbiota can be considered a real organ coordinating health and wellness of our body. It is made of more than 100 trillions of microorganisms, thus about 3 times higher than the number of human body cells and more than 150 times than human genes containing 1000 different microbe species. It has been described a symbiotic relationship between gut and kidney, confirmed by several observations. This is a bi-directional relation with a mutual influence, even when kidney disease occurs, and consequent alterations of intestinal microbiota and production of uremic toxins, that in turn worsens kidney disease and its progression. Our review analyzes the components of gut-kidney axis and relative clinical consequences. Copyright by Società Italiana di Nefrologia SIN, Rome, Italy.

Front-line window therapy with cisplatin in patients with primary disseminated Ewing sarcoma: A study by the Associazione Italiana di Ematologia ed Oncologia Pediatrica and Italian Sarcoma Group.

PubMed

Luksch, Roberto; Grignani, Giovanni; D'Angelo, Paolo; Prete, Arcangelo; Puma, Nadia; Podda, Marta; Casanova, Michela; Ferrari, Andrea; Morosi, Carlo; Fagioli, Franca; Aglietta, Massimo; Ferrari, Stefano; Picci, Piero; Massimino, Maura

2017-12-01

The aim was to assess the activity of cisplatin (CDDP) in Ewing sarcoma (ES). The study consisted of front-line window therapy with CDDP 120 mg/sqm every 3 weeks for two courses in children and young adults with primary disseminated ES. Response was assessed using the Response Evaluation Criteria in Solid Tumours criteria, and Simon's two-stage design was applied. Twelve consecutive patients were enrolled in stage 1. Only one objective response was observed. Since the target response rate was not achieved, accrual was stopped and CDDP as a single agent in ES was judged unworthy of further assessment. © 2017 Wiley Periodicals, Inc.

Adolescents with Cancer in Italy: Improving Access to National Cooperative Pediatric Oncology Group (AIEOP) Centers.

PubMed

Ferrari, Andrea; Rondelli, Roberto; Pession, Andrea; Mascarin, Maurizio; Buzzoni, Carlotta; Mosso, Maria Luisa; Maule, Milena; Barisone, Elena; Bertolotti, Marina; Clerici, Carlo Alfredo; Jankovic, Momcilo; Fagioli, Franca; Biondi, Andrea

2016-06-01

This analysis compared the numbers of patients treated at Italian pediatric oncology group (Associazione Italiana Ematologia Oncologia Pediatrica [AIEOP]) centers with the numbers of cases predicted according to the population-based registry. It considered 32,431 patients registered in the AIEOP database (1989-2012). The ratio of observed (O) to expected (E) cases was 0.79 for children (0-14 years old) and 0.15 for adolescents (15-19 years old). The proportion of adolescents increased significantly over the years, however, from 0.05 in the earliest period to 0.10, 0.18, and then 0.28 in the latest period of observation, suggesting a greater efficacy of local/national programs dedicated to adolescents. © 2016 Wiley Periodicals, Inc.

Attitudes of Italian general practitioners in the treatment of cancer pain. The Committee of the Associazione Italiana di Oncologia Medica (AIOM).

PubMed

Minotti, V; Betti, M

1997-01-01

The attitude of Italian general practitioners in prescribing practices for patients with cancer pain was assessed by means of a questionnaire. The results indicated that among most of the doctors who completed the questionnaire the basic principles of pain treatment in cancer patients are largely understood. Oral morphine emerged as the most commonly used opioid (60%) and controlled-release morphine as the preferred preparation. Non-steroidal anti-inflammatory drugs were the most commonly used minor analgesics. Fear of side effects and restrictive prescribing regulations emerged as the most important barrier against adequate pain management. The survey emphasised the need for continued efforts in implementing specific educational programming for improvement in cancer pain management.

Gastric lymphoma: the histology report.

PubMed

Doglioni, Claudio; Ponzoni, Maurilio; Ferreri, Andrés J M; Savio, Antonella

2011-03-01

The diagnosis of gastric MALT lymphoma is frequently difficult for the general histopathologist. During recent years there have been relevant changes in the therapeutic approach to gastric MALT lymphoma and our knowledge about its pathogenesis has greatly improved. The management of this disease actually requires a close cooperation between the histopathologist and the clinicians. The histology report of biopsies of a newly diagnosed or of an already treated case implies information of clinical and therapeutical relevance. This paper aims at giving the histopathologist a general knowledge about the state of art of this disease and its management. The diagnostic process leading to a complete and competent report is then described step by step. Copyright © 2011 Editrice Gastroenterologica Italiana S.r.l. Published by Elsevier Ltd.. All rights reserved.

The American nursing shortage during World War I: the debate over the use of nurses' aids.

PubMed

Telford, Jennifer Casavant

2010-01-01

This article explores the history of the creation of the Army and Navy Female Nurse Corps and the debate that ensued between American nursing leaders Jane Delano, director of the Red Cross Nursing Service, M. Adelaide Nutting, president of the American Federation of Nurses, and Annie Goodrich, dean of the Army School of Nursing, over the use of untrained nurses' aids to offset the nursing shortage that resulted from the United States entry into the Great War in 1917. The recruitment of minimally or untrained nurses' aids to offset the nursing shortage of the World War I era was a logical solution for American nursing leaders who had to meet the needs for nursing personnel. The exclusion of trained African American nurses, however, was a gross oversight on the part of these leaders. Whether or not this action compromised the status of nursing as a profession is still a matter of interest. Moreover, the debate about the delivery of care by unlicensed personnel continues.

Transforming individual civic engagement into community collective efficacy: the role of bonding social capital.

PubMed

Collins, Charles R; Neal, Jennifer Watling; Neal, Zachary P

2014-12-01

Collective efficacy is defined as residents' perceived collective capacity to take coordinated and interdependent action on issues that affect their lives. This study explored factors associated with neighborhood collective efficacy among residents. Utilizing a national sample of 4,120 urban households provided by Annie E. Casey Foundation's Making Connection Initiative, we investigated the mediating role of residents' perceptions of bonding social capital (i.e. reciprocity, trust, and shared norms) in the association between civic engagement and collective efficacy. Multiple regression analyses revealed that civic engagement and bonding social capital were both directly related to collective efficacy. Additionally, bonding social capital partially mediated the relationship between civic engagement and collective efficacy. Specifically, residents who reported greater levels of civic engagement also reported higher levels of bonding social capital. In turn, residents who reported higher levels of bonding social capital also reported higher levels of neighborhood collective efficacy. We discuss implications of these findings for researchers and practitioners interested in associations of neighborhood collective efficacy.

STS-95 Payload Specialist Glenn and his wife pose before their return flight to JSC

NASA Technical Reports Server (NTRS)

1998-01-01

At the Skid Strip at Cape Canaveral Air Station, STS-95 Payload Specialist John H. Glenn Jr., a senator from Ohio and one of the original seven Project Mercury astronauts, poses with his wife Annie before their return flight to the Johnson Space Center in Houston, Texas. The STS-95 mission ended with landing at Kennedy Space Center's Shuttle Landing Facility at 12:04 p.m. EST on Nov. 7. The STS-95 crew also includes Mission Commander Curtis L. Brown Jr.; Pilot Steven W. Lindsey; Mission Specialist Scott E. Parazynski; Mission Specialist Stephen K. Robinson; Mission Specialist Pedro Duque, with the European Space Agency (ESA); and Payload Specialist Chiaki Mukai, with the National Space Development Agency of Japan (NASDA). The mission included research payloads such as the Spartan-201 solar-observing deployable spacecraft, the Hubble Space Telescope Orbital Systems Test Platform, the International Extreme Ultraviolet Hitchhiker, as well as a SPACEHAB single module with experiments on space flight and the aging process.

Structure and Soot Properties of Nonbuoyant Ethylene/Air Laminar Jet Diffusion Flames. Appendix I

NASA Technical Reports Server (NTRS)

Urban, D. L.; Yuan, Z.-G.; Sunderland, P. B.; Linteris, G. T.; Voss, J. E.; Lin, K.-C.; Dai, Z.; Sun, K.; Faeth, G. M.; Ross, Howard D. (Technical Monitor)

2000-01-01

The structure and soot properties of round, soot-emitting, nonbuoyant, laminar jet diffusion flames are described, based on long-duration (175-230/s) experiments at microgravity carried out on orbit In the Space Shuttle Columbia. Experiments] conditions included ethylene-fueled flames burning in still air at nominal pressures of 50 and 100 kPa and an ambient temperature of 300 K with luminous Annie lengths of 49-64 mm. Measurements included luminous flame shapes using color video imaging, soot concentration (volume fraction) distributions using deconvoluted laser extinction imaging, soot temperature distributions using deconvoluted multiline emission imaging, gas temperature distributions at fuel-lean (plume) conditions using thermocouple probes, not structure distributions using thermophoretic sampling and analysis by transmission electron microscopy, and flame radiation using a radiometer. The present flames were larger, and emitted soot men readily, than comparable observed during ground-based microgravity experiments due to closer approach to steady conditions resulting from the longer test times and the reduced gravitational disturbances of the space-based experiments.

[Un processo che può gettare luce sulla cosiddetta 'paura di sé' nel disturbo ossessivo-compulsivo: l'Identificazione Retrospettiva delle Motivazioni e delle Inclinazioni].

PubMed

Mannino, Gherardo; Guerini, Rossella

2018-01-01

RIASSUNTO. Al fine di comprendere meglio la psicopatologia del disturbo ossessivo-compulsivo (DOC), in anni recenti è stata prestata una crescente attenzione alla cosiddetta "paura di sé" (fear of self), vale a dire la paura che le persone con DOC nutrono di ospitare dentro di sé aspetti inaccettabili. Tuttavia, l'esatta dinamica di questo fenomeno ancora non è chiara: di conseguenza, allo scopo di delucidarla meglio, sarà qui descritto uno specifico processo psicologico denominato Identificazione Retrospettiva delle Motivazioni e delle Inclinazioni (IRMI). Quando un paziente con DOC valuta in qualche modo come inaccettabile una propria esperienza interna (pensieri, emozioni, immagini mentali, ecc.) o un proprio comportamento, identificherà retrospettivamente le presunte motivazioni/inclinazioni negative che sarebbero state all'origine di quell'esperienza o comportamento. L'IRMI non solo aiuta a comprendere meglio il fenomeno della "paura di sé", ma possiede anche importanti implicazioni per la terapia.

Long-term analysis of the NOA-04 randomized phase III trial of sequential radiochemotherapy of anaplastic glioma with PCV or temozolomide.

PubMed

Wick, Wolfgang; Roth, Patrick; Hartmann, Christian; Hau, Peter; Nakamura, Makoto; Stockhammer, Florian; Sabel, Michael C; Wick, Antje; Koeppen, Susanne; Ketter, Ralf; Vajkoczy, Peter; Eyupoglu, Ilker; Kalff, Rolf; Pietsch, Torsten; Happold, Caroline; Galldiks, Norbert; Schmidt-Graf, Friederike; Bamberg, Michael; Reifenberger, Guido; Platten, Michael; von Deimling, Andreas; Meisner, Christoph; Wiestler, Benedikt; Weller, Michael

2016-11-01

Optimal treatment and precise classification for anaplastic glioma are needed. The objective for long-term follow-up of NOA-04 is to optimize the treatment sequence for patients with anaplastic gliomas. Patients were randomized 2:1:1 to receive the standard radiotherapy (RT) (arm A), procarbazine, lomustine and vincristine (PCV) (arm B1), or temozolomide (TMZ) (arm B2). Primary endpoint was time-to-treatment-failure (TTF), defined as progression after 2 lines of therapy or any time before if no further therapy was administered. Exploratory analyses examined associations of molecular marker status with TTF, progression-free survival (PFS), and overall survival (OS). At 9.5 (95% CI: 8.6-10.2) years, no difference between arms (A vs B1/B2) was observed: median TTF (4.6 [3.4-5.1] y vs 4.4 [3.3-5.3) y), PFS (2.5 [1.3-3.5] y vs 2.7 [1.9-3.2] y), and OS (8 [5.5-10.3] y vs 6.5 [5.4-8.3] y). Oligodendroglial versus astrocytic histology-but more so the subgroups according to CpG island methylator phenotype (CIMP) and 1p/19q co-deletion status-revealed a strong prognostic value of CIMP pos with (CIMP codel ) versus without 1p/19 co-deletion (CIMP non-codel ) versus CIMP neg . but no differential efficacy of RT versus chemotherapy for any of the endpoints. PFS was better for PCV- than for TMZ-treated patients with CIMP codel tumors (HR B1 vs B2 0.39 [0.17-0.92], P = .031). In CIMP neg . tumors, hypermethylation of the O6-methyl-guanyl-DNA methyltransferase promoter (MGMT) provided a risk reduction for PFS with chemotherapy. There is no differential activity of primary chemotherapy versus RT in any subgroup of anaplastic glioma. Molecular diagnosis is superior to histology. clinicaltrials.gov Identifier: NCT00717210. © The Author(s) 2016. Published by Oxford University Press on behalf of the Society for Neuro-Oncology. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

A gene expression signature of retinoblastoma loss-of-function is a predictive biomarker of resistance to palbociclib in breast cancer cell lines and is prognostic in patients with ER positive early breast cancer.

PubMed

Malorni, Luca; Piazza, Silvano; Ciani, Yari; Guarducci, Cristina; Bonechi, Martina; Biagioni, Chiara; Hart, Christopher D; Verardo, Roberto; Di Leo, Angelo; Migliaccio, Ilenia

2016-09-13

Palbociclib is a CDK4/6 inhibitor that received FDA approval for treatment of hormone receptor positive (HR+) HER2 negative (HER2neg) advanced breast cancer. To better personalize patients treatment it is critical to identify subgroups that would mostly benefit from it. We hypothesize that complex alterations of the Retinoblastoma (Rb) pathway might be implicated in resistance to CDK4/6 inhibitors and aim to investigate whether signatures of Rb loss-of-function would identify breast cancer cell lines resistant to palbociclib. We established a gene expression signature of Rb loss-of-function (RBsig) by identifying genes correlated with E2F1 and E2F2 expression in breast cancers within The Cancer Genome Atlas. We assessed the RBsig prognostic role in the METABRIC and in a comprehensive breast cancer meta-dataset. Finally, we analyzed whether RBsig would discriminate palbociclib-sensitive and -resistant breast cancer cells in a large RNA sequencing-based dataset. The RBsig was associated with RB1 genetic status in all tumors (p <7e-32) and in luminal or basal subtypes (p < 7e-11 and p < 0.002, respectively). The RBsig was prognostic in the METABRIC dataset (discovery: HR = 1.93 [1.5-2.4] p = 1.4e-08; validation: HR = 2.01 [1.6-2.5] p = 1.3e-09). Untreated and endocrine treated patients with estrogen receptor positive breast cancer expressing high RBsig had significantly worse recurrence free survival compared to those with low RBsig (HR = 2.37 [1.8 - 3.2] p = 1.87e-08 and HR = 2.62 [1.9- 3.5] p = 8.6e-11, respectively). The RBsig was able to identify palbociclib resistant and sensitive breast cancer cells (ROC AUC = 0,7778). Signatures of RB loss might be helpful in personalizing treatment of patients with HR+/HER2neg breast cancer. Further validation in patients receiving palbociclib is warranted.

Rosebud Sioux Wind Energy Project

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tony Rogers

2008-04-30

In 1998, through the vision of the late Alex “Little Soldier” Lunderman (1928-2000) and through the efforts of the Rosebud Sioux Tribal Utilities Commission, and with assistance from Intertribal Council on Utility Policy (COUP), and Distributed Generation, Inc (DISGEN). The Rosebud Sioux Tribe applied and was awarded in 1999 a DOE Cooperative Grant to build a commercial 750 Kw wind turbine, along with a 50/50 funding grant from the Department of Energy and a low interest loan from the Rural Utilities Service, United States Department of Agriculture, the Rosebud Sioux Tribe commissioned a single 750 kilowatt NEG Micon wind turbinemore » in March of 2003 near the Rosebud Casino. The Rosebud Sioux Wind Energy Project (Little Soldier “Akicita Cikala”) Turbine stands as a testament to the vision of a man and the Sicangu Oyate.« less

On the reliability of the CD123-endowed basophil activation test (BAT) and its application in food allergy.

PubMed

Chirumbolo, Salvatore; Bjørklund, Geir; Vella, Antonio

2018-06-15

the recent paper by Appel et al., evaluated the use of the basophil activaion test (BAT) in sesame allergy and concluded that BAT should be preferentially used in association with high protein sesame extract-skin prick test (HSPE-SPT) for the diagnosis of sesame food allergy, so preventing any ethical issue regarding the possble risk associated with oral food challenge (OFC) 1 . The authors used a non commercial BAT approach endowed with the CD123 pos /HLADR neg phenotyping protocol and using CD63 and/or CD203c as activation markers 2,3 . Basophils were initially captured in flow cytometry (FC) from a not clearly separated side scatter (SSC)-CD123 pos . This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.

KSC-98pc1776

NASA Image and Video Library

1998-12-03

KENNEDY SPACE CENTER, FLA. -- Participants pose for a photo at the Space Station Processing Facility ceremony transferring the "Leonardo" Multipurpose Logistics Module (MPLM) from the Italian Space Agency, Agenzia Spaziale Italiana (ASI), to NASA. From left, they are astronaut Jim Voss, European Space Agency astronauts Umberto Guidoni of Italy and Christer Fuglesang of Sweden, NASA International Space Station Program Manager Randy Brinkley, NASA Administrator Daniel S. Goldin, ASI President Sergio De Julio and Stephen Francois, director, International Space Station Launch Site Support at KSC. The MPLM, a reusable logistics carrier, will be the primary delivery system used to resupply and return International Space Station cargo requiring a pressurized environment. Leonardo is the first of three MPLM carriers for the International Space Station. It is scheduled to be launched on Space Shuttle Mission STS-100, targeted for April 2000

Boron neutron capture therapy (BNCT) in Finland: technological and physical prospects after 20 years of experiences.

PubMed

Savolainen, Sauli; Kortesniemi, Mika; Timonen, Marjut; Reijonen, Vappu; Kuusela, Linda; Uusi-Simola, Jouni; Salli, Eero; Koivunoro, Hanna; Seppälä, Tiina; Lönnroth, Nadja; Välimäki, Petteri; Hyvönen, Heini; Kotiluoto, Petri; Serén, Tom; Kuronen, Antti; Heikkinen, Sami; Kosunen, Antti; Auterinen, Iiro

2013-05-01

Boron Neutron Capture Therapy (BNCT) is a binary radiotherapy method developed to treat patients with certain malignant tumours. To date, over 300 treatments have been carried out at the Finnish BNCT facility in various on-going and past clinical trials. In this technical review, we discuss our research work in the field of medical physics to form the groundwork for the Finnish BNCT patient treatments, as well as the possibilities to further develop and optimize the method in the future. Accordingly, the following aspects are described: neutron sources, beam dosimetry, treatment planning, boron imaging and determination, and finally the possibilities to detect the efficacy and effects of BNCT on patients. Copyright © 2012 Associazione Italiana di Fisica Medica. Published by Elsevier Ltd. All rights reserved.

Chronic idiopathic inflammatory bowel diseases: the histology report.

PubMed

Cornaggia, Matteo; Leutner, Monica; Mescoli, Claudia; Sturniolo, Giacomo Carlo; Gullotta, Renzo

2011-03-01

The incidence of chronic idiopathic inflammatory bowel diseases (IBD) is growing in western countries, making their histological diagnosis an everyday task for all pathologists. Reviews from the literature strongly suggest that such diagnosis cannot be performed on the histological ground alone but requires a clinical-pathological approach. Moreover, bewildering variations can be observed in the terminology employed to report either individual lesions or diagnostic categories. The aim of the present paper is to suggest a practical diagnostic algorithm summarizing the main data from the literature. Particular emphasis has been placed on minimum clinical information required and the accurate definition of individual lesions. Diagnostic categories to employ and to avoid in daily practice have furthermore been stressed. Copyright © 2011 Editrice Gastroenterologica Italiana S.r.l. Published by Elsevier Ltd.. All rights reserved.

Pearson Syndrome: A Retrospective Cohort Study from the Marrow Failure Study Group of A.I.E.O.P. (Associazione Italiana Emato-Oncologia Pediatrica).

PubMed

Farruggia, Piero; Di Cataldo, Andrea; Pinto, Rita M; Palmisani, Elena; Macaluso, Alessandra; Valvo, Laura Lo; Cantarini, Maria E; Tornesello, Assunta; Corti, Paola; Fioredda, Francesca; Varotto, Stefania; Martire, Baldo; Moroni, Isabella; Puccio, Giuseppe; Russo, Giovanna; Dufour, Carlo; Pillon, Marta

2016-01-01

Pearson syndrome (PS) is a very rare and often fatal multisystemic mitochondrial disorder involving the liver, kidney, pancreas, and hematopoietic and central nervous system. It is characterized principally by a transfusion-dependent anemia that usually improves over time, a tendency to develop severe infections, and a high mortality rate. We describe a group of 11 PS patients diagnosed in Italy in the period 1993-2014. The analysis of this reasonably sized cohort of patients contributes to the clinical profile of the disease and highlights a rough incidence of 1 case/million newborns. Furthermore, it seems that some biochemical parameters like increased serum alanine and urinary fumaric acid can help to address an early diagnosis.

[Cardio-renal axis: pathophysiological evidences and clinical implications].

PubMed

Di Lullo, Luca; Ronco, Claudio

2017-03-01

According to the recent definition proposed by the Consensus conference on Acute Dialysis Quality Initiative Group, the term cardio-renal syndrome CRS has been used to define different clinical conditions in which heart and kidney dysfunction overlap. Type 1 CRS acute cardio - renal syndrome is characterized by acute worsening of cardiac function leading to AKI in the setting of active cardiac disease such as ADHF, while type - 2 CRS occurs in a setting of chronic heart disease. Type 3 CRS is closely link to acute kidney injury, while type 4 represent cardiovascular involvement in chronic kidney disese patients. Type 5 CRS represent cardiac and renal involvement in several diseases such as sepsis, hepato - renal syndrome and immune - mediated diseases. Copyright by Società Italiana di Nefrologia SIN, Rome, Italy.

Toward precision medicine in primary biliary cholangitis.

PubMed

Carbone, Marco; Ronca, Vincenzo; Bruno, Savino; Invernizzi, Pietro; Mells, George F

2016-08-01

Primary biliary cholangitis is a chronic, cholestatic liver disease characterized by a heterogeneous presentation, symptomatology, disease progression and response to therapy. In contrast, clinical management and treatment of PBC is homogeneous with a 'one size fits all' approach. The evolving research landscape, with the emergence of the -omics field and the availability of large patient cohorts are creating a unique opportunity of translational epidemiology. Furthermore, several novel disease and symptom-modifying agents for PBC are currently in development. The time is therefore ripe for precision medicine in PBC. In this manuscript we describe the concept of precision medicine; review current approaches to risk-stratification in PBC, and speculate how precision medicine in PBC might develop in the near future. Copyright © 2016 Editrice Gastroenterologica Italiana S.r.l. Published by Elsevier Ltd. All rights reserved.