Sample records for ja farm plant

  1. Organic farming benefits local plant diversity in vineyard farms located in intensive agricultural landscapes.

    PubMed

    Nascimbene, Juri; Marini, Lorenzo; Paoletti, Maurizio G

    2012-05-01

    The majority of research on organic farming has considered arable and grassland farming systems in Central and Northern Europe, whilst only a few studies have been carried out in Mediterranean agro-systems, such as vineyards, despite their economic importance. The main aim of the study was to test whether organic farming enhances local plant species richness in both crop and non-crop areas of vineyard farms located in intensive conventional landscapes. Nine conventional and nine organic farms were selected in an intensively cultivated region (i.e. no gradient in landscape composition) in northern Italy. In each farm, vascular plants were sampled in one vineyard and in two non-crop linear habitats, grass strips and hedgerows, adjacent to vineyards and therefore potentially influenced by farming. We used linear mixed models to test the effect of farming, and species longevity (annual vs. perennial) separately for the three habitat types. In our intensive agricultural landscapes organic farming promoted local plant species richness in vineyard fields, and grassland strips while we found no effect for linear hedgerows. Differences in species richness were not associated to differences in species composition, indicating that similar plant communities were hosted in vineyard farms independently of the management type. This negative effect of conventional farming was probably due to the use of herbicides, while mechanical operations and mowing regime did not differ between organic and conventional farms. In grassland strips, and only marginally in vineyards, we found that the positive effect of organic farming was more pronounced for perennial than annual species.

  2. MAPK-dependent JA and SA signalling in Nicotiana attenuata affects plant growth and fitness during competition with conspecifics

    PubMed Central

    2012-01-01

    Background Induced defense responses to herbivores are generally believed to have evolved as cost-saving strategies that defer the fitness costs of defense metabolism until these defenses are needed. The fitness costs of jasmonate (JA)-mediated defenses have been well documented. Those of the early signaling units mediating induced resistance to herbivores have yet to be examined. Early signaling components that mediate herbivore-induced defense responses in Nicotiana attenuata, have been well characterized and here we examine their growth and fitness costs during competition with conspecifics. Two mitogen-activated protein kinases (MAPKs), salicylic acid (SA)-induced protein kinase (SIPK) and wound-induced protein kinase (WIPK) are rapidly activated after perception of herbivory and both kinases regulate herbivory-induced JA levels and JA-mediated defense metabolite accumulations. Since JA-induced defenses result in resource-based trade-offs that compromise plant productivity, we evaluated if silencing SIPK (irSIPK) and WIPK (irWIPK) benefits the growth and fitness of plants competiting with wild type (WT) plants, as has been shown for plants silenced in JA-signaling by the reduction of Lipoxygenase 3 (LOX3) levels. Results As expected, irWIPK and LOX3-silenced plants out-performed their competing WT plants. Surprisingly, irSIPK plants, which have the largest reductions in JA signaling, did not. Phytohormone profiling of leaves revealed that irSIPK plants accumulated higher levels of SA compared to WT. To test the hypothesis that these high levels of SA, and their presumed associated fitness costs of pathogen associated defenses in irSIPK plants had nullified the JA-deficiency-mediated growth benefits in these plants, we genetically reduced SA levels in irSIPK plants. Reducing SA levels partially recovered the biomass and fitness deficits of irSIPK plants. We also evaluated whether the increased fitness of plants with reduced SA or JA levels resulted from

  3. Use of plant roots for phytoremediation and molecular farming

    PubMed Central

    Gleba, Doloressa; Borisjuk, Nikolai V.; Borisjuk, Ludmyla G.; Kneer, Ralf; Poulev, Alexander; Skarzhinskaya, Marina; Dushenkov, Slavik; Logendra, Sithes; Gleba, Yuri Y.; Raskin, Ilya

    1999-01-01

    Alternative agriculture, which expands the uses of plants well beyond food and fiber, is beginning to change plant biology. Two plant-based biotechnologies were recently developed that take advantage of the ability of plant roots to absorb or secrete various substances. They are (i) phytoextraction, the use of plants to remove pollutants from the environment and (ii) rhizosecretion, a subset of molecular farming, designed to produce and secrete valuable natural products and recombinant proteins from roots. Here we discuss recent advances in these technologies and assess their potential in soil remediation, drug discovery, and molecular farming. PMID:10339526

  4. Orgenic plants: gene-manipulated plants compatible with organic farming.

    PubMed

    Ryffel, Gerhart U

    2012-11-01

    Based on recent advances in plant gene technology, I propose to develop a new category of GM plants, orgenic plants, that are compatible with organic farming. These orgenic plants do not contain herbicide resistance genes to avoid herbicide application in agriculture. Furthermore, they either contain genes that are naturally exchanged between species, or are sterile to avoid outcrossing if they received a transgene from a different species. These GM plants are likely to be acceptable to most skeptics of GM plants and facilitate the use of innovative new crops. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  5. Effects of MeJA on Arabidopsis metabolome under endogenous JA deficiency

    NASA Astrophysics Data System (ADS)

    Cao, Jingjing; Li, Mengya; Chen, Jian; Liu, Pei; Li, Zhen

    2016-11-01

    Jasmonates (JAs) play important roles in plant growth, development and defense. Comprehensive metabolomics profiling of plants under JA treatment provides insights into the interaction and regulation network of plant hormones. Here we applied high resolution mass spectrometry based metabolomics approach on Arabidopsis wild type and JA synthesis deficiency mutant opr3. The effects of exogenous MeJA treatment on the metabolites of opr3 were investigated. More than 10000 ion signals were detected and more than 2000 signals showed significant variation in different genotypes and treatment groups. Multivariate statistic analyses (PCA and PLS-DA) were performed and a differential compound library containing 174 metabolites with high resolution precursor ion-product ions pairs was obtained. Classification and pathway analysis of 109 identified compounds in this library showed that glucosinolates and tryptophan metabolism, amino acids and small peptides metabolism, lipid metabolism, especially fatty acyls metabolism, were impacted by endogenous JA deficiency and exogenous MeJA treatment. These results were further verified by quantitative reverse transcription PCR (RT-qPCR) analysis of 21 related genes involved in the metabolism of glucosinolates, tryptophan and α-linolenic acid pathways. The results would greatly enhance our understanding of the biological functions of JA.

  6. [Storage of plant protection products in farms: minimum safety requirements].

    PubMed

    Dutto, Moreno; Alfonzo, Santo; Rubbiani, Maristella

    2012-01-01

    Failure to comply with requirements for proper storage and use of pesticides in farms can be extremely hazardous and the risk of accidents involving farm workers, other persons and even animals is high. There are still wide differences in the interpretation of the concept of "securing or making safe", by workers in this sector. One of the critical points detected, particularly in the fruit sector, is the establishment of an adequate storage site for plant protection products. The definition of "safe storage of pesticides" is still unclear despite the recent enactment of Legislative Decree 81/2008 regulating health and work safety in Italy. In addition, there are no national guidelines setting clear minimum criteria for storage of plant protection products in farms. The authors, on the basis of their professional experience and through analysis of recent legislation, establish certain minimum safety standards for storage of pesticides in farms.

  7. Arabidopsis GOLDEN2-LIKE (GLK) transcription factors activate jasmonic acid (JA)-dependent disease susceptibility to the biotrophic pathogen Hyaloperonospora arabidopsidis, as well as JA-independent plant immunity against the necrotrophic pathogen Botrytis cinerea.

    PubMed

    Murmu, Jhadeswar; Wilton, Michael; Allard, Ghislaine; Pandeya, Radhey; Desveaux, Darrell; Singh, Jas; Subramaniam, Rajagopal

    2014-02-01

    Arabidopsis thaliana GOLDEN2-LIKE (GLK1 and 2) transcription factors regulate chloroplast development in a redundant manner. Overexpression of AtGLK1 (35S:AtGLK1) in Arabidopsis also confers resistance to the cereal pathogen Fusarium graminearum. To further elucidate the role of GLK transcription factors in plant defence, the Arabidopsis glk1 glk2 double-mutant and 35S:AtGLK1 plants were challenged with the virulent oomycete pathogen Hyaloperonospora arabidopsidis (Hpa) Noco2. Compared with Col-0, glk1 glk2 plants were highly resistant to Hpa Noco2, whereas 35S:AtGLK1 plants showed enhanced susceptibility to this pathogen. Genetic studies suggested that AtGLK-mediated plant defence to Hpa Noco2 was partially dependent on salicylic acid (SA) accumulation, but independent of the SA signalling protein NONEXPRESSOR OF PATHOGENESIS-RELATED 1 (NPR1). Pretreatment with jasmonic acid (JA) dramatically reversed Hpa Noco2 resistance in the glk1 glk2 double mutant, but only marginally affected the 35S:AtGLK1 plants. In addition, overexpression of AtGLK1 in the JA signalling mutant coi1-16 did not increase susceptibility to Hpa Noco2. Together, our GLK gain-of-function and loss-of-function experiments suggest that GLK acts upstream of JA signalling in disease susceptibility to Hpa Noco2. In contrast, glk1 glk2 plants were more susceptible to the necrotrophic fungal pathogen Botrytis cinerea, whereas 35S:AtGLK1 plants exhibited heightened resistance which could be maintained in the absence of JA signalling. Together, the data reveal that AtGLK1 is involved in JA-dependent susceptibility to the biotrophic pathogen Hpa Noco2 and in JA-independent resistance to the necrotrophic pathogen B. cinerea. © 2013 HER MAJESTY THE QUEEN IN RIGHT OF CANADA. MOLECULAR PLANT PATHOLOGY © 2013 BSPP. REPRODUCED WITH THE PERMISSION OF THE MINISTER OF AGRICULTURE AND AGRI-FOOD CANADA.

  8. Development of Farming Diversification with Implementation Plant Patterns as a Strategy of Economic Strengthening

    NASA Astrophysics Data System (ADS)

    Anwar, S.; Setyohadi, D. P. S.; Utami, M. M. D.; Damanhuri; Hariono, B.

    2018-01-01

    Bojonegoro, Tulungagung, and Ponorogo districts are an agrarian area and become one of the leading food crops producers in East Java Province. Diversification of farming in this region is done by applying season-based cropping pattern, which is cultivating various commodities in rotation. Farmers need diversification programs wetland cannot provide an optimal contribution to the income of farmers caused because farmers are not able to cultivate high value-added commodities due to limited capital. This research is to identify the characteristics of farming and to analyse the farming system to know the pattern of planting suggestion and prospect. The research used descriptive method, profit farming analysis, and SWOT. The results showed that each region has a specific planting pattern with rice as the main commodity grown in the rainy season followed by crops and horticultural crops and a suggested planting pattern that needs to be implemented by farmers to increase their income. The prospect of diversification of farming development through the implementation of the proposed planting pattern is very suitable with the character of the region and the market demand.

  9. Plant Molecular Farming: Much More than Medicines.

    PubMed

    Tschofen, Marc; Knopp, Dietmar; Hood, Elizabeth; Stöger, Eva

    2016-06-12

    Plants have emerged as commercially relevant production systems for pharmaceutical and nonpharmaceutical products. Currently, the commercially available nonpharmaceutical products outnumber the medical products of plant molecular farming, reflecting the shorter development times and lower regulatory burden of the former. Nonpharmaceutical products benefit more from the low costs and greater scalability of plant production systems without incurring the high costs associated with downstream processing and purification of pharmaceuticals. In this review, we explore the areas where plant-based manufacturing can make the greatest impact, focusing on commercialized products such as antibodies, enzymes, and growth factors that are used as research-grade or diagnostic reagents, cosmetic ingredients, and biosensors or biocatalysts. An outlook is provided on high-volume, low-margin proteins such as industrial enzymes that can be applied as crude extracts or unprocessed plant tissues in the feed, biofuel, and papermaking industries.

  10. Plant Molecular Farming: Much More than Medicines

    NASA Astrophysics Data System (ADS)

    Tschofen, Marc; Knopp, Dietmar; Hood, Elizabeth; Stöger, Eva

    2016-06-01

    Plants have emerged as commercially relevant production systems for pharmaceutical and nonpharmaceutical products. Currently, the commercially available nonpharmaceutical products outnumber the medical products of plant molecular farming, reflecting the shorter development times and lower regulatory burden of the former. Nonpharmaceutical products benefit more from the low costs and greater scalability of plant production systems without incurring the high costs associated with downstream processing and purification of pharmaceuticals. In this review, we explore the areas where plant-based manufacturing can make the greatest impact, focusing on commercialized products such as antibodies, enzymes, and growth factors that are used as research-grade or diagnostic reagents, cosmetic ingredients, and biosensors or biocatalysts. An outlook is provided on high-volume, low-margin proteins such as industrial enzymes that can be applied as crude extracts or unprocessed plant tissues in the feed, biofuel, and papermaking industries.

  11. 109. OVERALL VIEW OF NORTH PLANT, WITH DICHLORO TANK FARM ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    109. OVERALL VIEW OF NORTH PLANT, WITH DICHLORO TANK FARM IN LEFT CENT FOREGROUND AND ASSEMBLY PLANT/WAREHOUSE (BUILDING 1601/1606/1701) BEHIND. VIEW TO NORTHEAST. - Rocky Mountain Arsenal, Bounded by Ninety-sixth Avenue & Fifty-sixth Avenue, Buckley Road, Quebec Street & Colorado Highway 2, Commerce City, Adams County, CO

  12. Flow Field Analysis of Fish Farm and Planting Area in Floodplain during Flood

    NASA Astrophysics Data System (ADS)

    Wu, M.; Tan, H. N.; Lo, W. C.; Tsai, C. T.

    2017-12-01

    Fish farms constructing and crops planting is common in floodplain in Taiwan. The physiographic soil erosion-deposition (PSED) model was applied to simulate the sediment yield, the runoff, and sediment transport rate of the river watershed corresponding to one-day rainstorms of the return periods of 25, 50, and 100 year. The variation of flow field in the river sections could be simulated by utilizing the alluvial river-movable bed two dimensional (ARMB-2D) model. The results reveal that the tendency of river discharge, sediment deposition and erosion obtained from these two models is agreeable by calibration and verification. The water flow affected by fish farms and planting areas in floodplain during flood was analyzed. Lastly, based on the simulation results obtained from the PESD and ARMB-2D models for one-day rainstorms of the return periods of 25, 50, and 100 year, the illegal fish farms and planting area with severe variations of river flow and affected he capability for flood conveyance will be referred to as the demolishing-to-be areas. We could also suggest the management strategy of application for fish farms constructing and crops planting in river areas by incorporating the ability of our model to provide information of river flow to enhance the flood conveyance.

  13. Wildlife-friendly farming benefits rare birds, bees and plants.

    PubMed

    Pywell, Richard F; Heard, Matthew S; Bradbury, Richard B; Hinsley, Shelley; Nowakowski, Marek; Walker, Kevin J; Bullock, James M

    2012-10-23

    Agricultural intensification is a leading cause of global biodiversity loss, especially for threatened and near-threatened species. One widely implemented response is 'wildlife-friendly farming', involving the close integration of conservation and extensive farming practices within agricultural landscapes. However, the putative benefits from this controversial policy are currently either unknown or thought unlikely to extend to rare and declining species. Here, we show that new, evidence-based approaches to habitat creation on intensively managed farmland in England can achieve large increases in plant, bee and bird species. In particular, we found that habitat enhancement methods designed to provide the requirements of sensitive target biota consistently increased the richness and abundance of both rare and common species, with 10-fold to greater than 100-fold more rare species per sample area than generalized conventional conservation measures. Furthermore, targeting landscapes of high species richness amplified beneficial effects on the least mobile taxa: plants and bees. Our results provide the first unequivocal support for a national wildlife-friendly farming policy and suggest that this approach should be implemented much more extensively to address global biodiversity loss. However, to be effective, these conservation measures must be evidence-based, and developed using sound knowledge of the ecological requirements of key species.

  14. OsMPK3 positively regulates the JA signaling pathway and plant resistance to a chewing herbivore in rice.

    PubMed

    Wang, Qi; Li, Jiancai; Hu, Lingfei; Zhang, Tongfang; Zhang, Guren; Lou, Yonggen

    2013-07-01

    KEY MESSAGE : Silencing OsMPK3 decreased elicited JA levels, which subsequently reduced levels of herbivore-induced trypsin protease inhibitors (TrypPIs) and improved the performance of SSB larvae, but did not influence BPH. Mitogen-activated protein kinases (MPKs) are known to play an important role in plant defense by transferring biotic and abiotic signals into programmed cellular responses. However, their functions in the herbivore-induced defense response in rice remain largely unknown. Here, we identified a MPK3 gene from rice, OsMPK3, and found that its expression levels were up-regulated in response to infestation by the larvae of the striped stem borer (SSB) (Chilo suppressalis), to mechanical wounding and to treatment with jasmonic acid (JA), but not to infestation by the brown planthopper (BPH) Nilaparvata lugens or to treatment with salicylic acid. Moreover, mechanical wounding and SSB infestation induced the expression of OsMPK3 strongly and quickly, whereas JA treatment induced the gene more weakly and slowly. Silencing OsMPK3 (ir-mpk3) reduced the expression of the gene by 50-70 %, decreased elicited levels of JA and diminished the expression of a lipoxygenase gene OsHI-LOX and an allene oxide synthase gene OsAOS1. The reduced JA signaling in ir-mpk3 plants decreased the levels of herbivore-induced trypsin protease inhibitors (TrypPIs) and improved the performance of SSB larvae, but did not influence BPH. Our findings suggest that the gene OsMPK3 responds early in herbivore-induced defense and can be regulated by rice plants to activate a specific and appropriate defense response to different herbivores.

  15. Cyclic lipopeptide iturin A structure-dependently induces defense response in Arabidopsis plants by activating SA and JA signaling pathways.

    PubMed

    Kawagoe, Yumi; Shiraishi, Soma; Kondo, Hiroko; Yamamoto, Shoko; Aoki, Yoshinao; Suzuki, Shunji

    2015-05-15

    Iturin A is the most well studied antifungal cyclic lipopeptide produced by Bacillus species that are frequently utilized as biological control agents. Iturin A not only shows strong antifungal activity against phytopathogens but also induces defense response in plants, thereby reducing plant disease severity. Here we report the defense signaling pathways triggered by iturin A in Arabidopsis salicylic acid (SA) or jasmonic acid (JA)-insensitive mutants. Iturin A activated the transcription of defense genes PR1 and PDF1.2 through the SA and JA signaling pathways, respectively. The role of iturin A as an elicitor was dependent on the cyclization of the seven amino acids and/or the β-hydroxy fatty acid chain. The iturin A derivative peptide, NH2-(L-Asn)-(D-Tyr)-(D-Asn)-(L-Gln)-(L-Pro)-(D-Asn)-(L-Ser)-COOH, completely suppressed PR1 and PDF1.2 gene expression in wild Arabidopsis plants. The identification of target molecules binding to iturin A and its derivative peptide is expected to shed new light on defense response in plants through the SA and JA signaling pathways. Copyright © 2015 Elsevier Inc. All rights reserved.

  16. Changes in ABA, IAA and JA levels during calyx, fruit and leaves development in cape gooseberry plants (Physalis peruviana L.).

    PubMed

    Álvarez-Flórez, F; López-Cristoffanini, C; Jáuregui, O; Melgarejo, L M; López-Carbonell, M

    2017-06-01

    Changes in abscisic acid (ABA), indole-3-acetic acid (IAA) and jasmonic acid (JA) content in developing calyx, fruits and leaves of Physalis peruviana L. plants were analysed. Plant hormones have been widely studied for their roles in the regulation of various aspects related to plant development and, in particular, into their action during development and ripening of fleshly fruits. The obtained evidences suggest that the functions of these hormones are no restricted to a particular development stage, and more than one hormone is involved in controlling various aspects of plant development. Our results will contribute to understand the role of these hormones during growth and development of calyx, fruits and leaves in cape gooseberry plants. This work offers a good, quickly and efficiently protocol to extract and quantify simultaneously ABA, IAA and JA in different tissues of cape gooseberry plants. Copyright © 2017 Elsevier Masson SAS. All rights reserved.

  17. Consequential environmental life cycle assessment of a farm-scale biogas plant.

    PubMed

    Van Stappen, Florence; Mathot, Michaël; Decruyenaere, Virginie; Loriers, Astrid; Delcour, Alice; Planchon, Viviane; Goffart, Jean-Pierre; Stilmant, Didier

    2016-06-15

    Producing biogas via anaerobic digestion is a promising technology for meeting European and regional goals on energy production from renewable sources. It offers interesting opportunities for the agricultural sector, allowing waste and by-products to be converted into bioenergy and bio-based materials. A consequential life cycle assessment (cLCA) was conducted to examine the consequences of the installation of a farm-scale biogas plant, taking account of assumptions about processes displaced by biogas plant co-products (power, heat and digestate) and the uses of the biogas plant feedstock prior to plant installation. Inventory data were collected on an existing farm-scale biogas plant. The plant inputs are maize cultivated for energy, solid cattle manure and various by-products from surrounding agro-food industries. Based on hypotheses about displaced electricity production (oil or gas) and the initial uses of the plant feedstock (animal feed, compost or incineration), six scenarios were analyzed and compared. Digested feedstock previously used in animal feed was replaced with other feed ingredients in equivalent feed diets, designed to take account of various nutritional parameters for bovine feeding. The displaced production of mineral fertilizers and field emissions due to the use of digestate as organic fertilizer was balanced against the avoided use of manure and compost. For all of the envisaged scenarios, the installation of the biogas plant led to reduced impacts on water depletion and aquatic ecotoxicity (thanks mainly to the displaced mineral fertilizer production). However, with the additional animal feed ingredients required to replace digested feedstock in the bovine diets, extra agricultural land was needed in all scenarios. Field emissions from the digestate used as organic fertilizer also had a significant impact on acidification and eutrophication. The choice of displaced marginal technologies has a huge influence on the results, as have the

  18. Peatland carbon cycling at a Scottish wind farm: the role of plant-soil interactions

    NASA Astrophysics Data System (ADS)

    Richardson, Harriett; Whitaker, Jeanette; Waldron, Susan; Ostle, Nick

    2013-04-01

    Peatlands play a fundamental role in the terrestrial carbon cycle by storing 1/3 of the world's soil carbon (Limpens et al. 2008). In the UK, peatlands are often located in areas with potential for electricity generation by harvesting wind energy. Concerns have been raised, however, over the stability of these carbon stocks when large scale wind developments are sited upon them. This project aims to improve understanding of the impact of wind farms on carbon sequestration in peatlands. Wind turbine 'wake-effects' can alter microclimatic conditions, as a result of significant differences in air temperature, humidity, wind speed and turbulence (Baidya Roy and Traiteur 2010). These changes are likely to have a significant impact on above and below ground abiotic conditions and biotic properties, together with the processes they regulate that govern peatland carbon cycling. Specifically, the effects of interactions between typical peatland plant functional types (graminoids, bryophytes and shrubs) (Ward et al. 2009) and peat microbial community composition and function are poorly resolved. We examined a spatial gradient across an area of blanket bog at Black Law wind farm (Lanarkshire, Scotland) and executed a series of controlled mesocosm experiments to examine the impacts of potential microclimatic changes on plant-soil interactions and carbon sequestration processes. In particular we focused on the form and function of plant and microbial communities as determinants of decomposition (Ward et al. 2010) and greenhouse gas (GHG) emissions (Artz 2009). Measurements of plant-litter-soil carbon, nitrogen, microbial community composition (i.e. phospholipid fatty acid biomarkers) and litter mass loss have been made across the wind farm peatland to attribute spatial variance in biotic and biogeochemical properties. In addition, multi-factorial mesocosm experiments have been made to determine how abiotic and biotic changes caused by wind farm effects could influence peat GHG

  19. Potential of plant polyphenols to combat oxidative stress and inflammatory processes in farm animals.

    PubMed

    Gessner, D K; Ringseis, R; Eder, K

    2017-08-01

    Polyphenols are secondary plant metabolites which have been shown to exert antioxidative and antiinflamma tory effects in cell culture, rodent and human studies. Based on the fact that conditions of oxidative stress and inflammation are highly relevant in farm animals, polyphenols are considered as promising feed additives in the nutrition of farm animals. However, in contrast to many studies existing with model animals and humans, potential antioxidative and antiinflammatory effects of polyphenols have been less investigated in farm animals so far. This review aims to give an overview about potential antioxidative and antiinflammatory effects in farm animals. The first part of the review highlights the occurrence and the consequences of oxidative stress and inflammation on animal health and performance. The second part of the review deals with bioavailability and metabolism of polyphenols in farm animals. The third and main part of the review presents an overview of the findings from studies which investigated the effects of polyphenols of various plant sources in pigs, poultry and cattle, with particular consideration of effects on the antioxidant system and inflammation. Journal of Animal Physiology and Animal Nutrition © 2016 Blackwell Verlag GmbH.

  20. Ecological analysis of a typical farm-scale biogas plant in China

    NASA Astrophysics Data System (ADS)

    Duan, Na; Lin, Cong; Wang, Pingzhi; Meng, Jing; Chen, Hui; Li, Xue

    2014-09-01

    The aim of this work was to present the common anaerobic digestion technologies in a typical farm-scale biogas plant in China. The comprehensive benefits of most biogas plants in China have not been fully assessed in past decades due to the limited information of the anaerobic digestion processes in biogas plants. This paper analyzed four key aspects (i.e., operational performance, nonrenewable energy (NE) savings, CO2 emission reduction (CER) and economic benefits (EBs)) of a typical farm-scale biogas plant, where beef cattle manure was used as feedstock. Owing to the monitoring system, stable operation was achieved with a hydraulic retention time of 18-22 days and a production of 876,000 m3 of biogas and 37,960 t of digestate fertilizer annually. This could substantially substitute for the nonrenewable energy and chemical fertilizer. The total amount of NE savings and CER derived from biogas and digestate fertilizer was 2.10×107 MJ (equivalent to 749.7 tce) and 9.71×105 kg, respectively. The EBs of the biogas plant was 6.84×105 CNY·yr-1 with an outputs-to-inputs ratio of 2.37. As a result, the monitoring system was proved to contribute significantly to the sound management and quantitative assessment of the biogas plant. Biogas plants could produce biogas which could be used to substitute fossil fuels and reduce the emissions of greenhouse gases, and digestate fertilizer is also an important bio-product.

  1. Embedded mobile farm robot for identification of diseased plants

    NASA Astrophysics Data System (ADS)

    Sadistap, S. S.; Botre, B. A.; Pandit, Harshavardhan; Chandrasekhar; Rao, Adesh

    2013-07-01

    This paper presents the development of a mobile robot used in farms for identification of diseased plants. It puts forth two of the major aspects of robotics namely automated navigation and image processing. The robot navigates on the basis of the GPS (Global Positioning System) location and data obtained from IR (Infrared) sensors to avoid any obstacles in its path. It uses an image processing algorithm to differentiate between diseased and non-diseased plants. A robotic platform consisting of an ARM9 processor, motor drivers, robot mechanical assembly, camera and infrared sensors has been used. Mini2440 microcontroller has been used wherein Embedded linux OS (Operating System) is implemented.

  2. Phage Therapy and Photodynamic Therapy: Low Environmental Impact Approaches to Inactivate Microorganisms in Fish Farming Plants

    PubMed Central

    Almeida, Adelaide; Cunha, Ângela; Gomes, Newton C.M.; Alves, Eliana; Costa, Liliana; Faustino, Maria A.F.

    2009-01-01

    Owing to the increasing importance of aquaculture to compensate for the progressive worldwide reduction of natural fish and to the fact that several fish farming plants often suffer from heavy financial losses due to the development of infections caused by microbial pathogens, including multidrug resistant bacteria, more environmentally-friendly strategies to control fish infections are urgently needed to make the aquaculture industry more sustainable. The aim of this review is to briefly present the typical fish farming diseases and their threats and discuss the present state of chemotherapy to inactivate microorganisms in fish farming plants as well as to examine the new environmentally friendly approaches to control fish infection namely phage therapy and photodynamic antimicrobial therapy. PMID:19841715

  3. Wildlife-friendly farming benefits rare birds, bees and plants

    PubMed Central

    Pywell, Richard F.; Heard, Matthew S.; Bradbury, Richard B.; Hinsley, Shelley; Nowakowski, Marek; Walker, Kevin J.; Bullock, James M.

    2012-01-01

    Agricultural intensification is a leading cause of global biodiversity loss, especially for threatened and near-threatened species. One widely implemented response is ‘wildlife-friendly farming’, involving the close integration of conservation and extensive farming practices within agricultural landscapes. However, the putative benefits from this controversial policy are currently either unknown or thought unlikely to extend to rare and declining species. Here, we show that new, evidence-based approaches to habitat creation on intensively managed farmland in England can achieve large increases in plant, bee and bird species. In particular, we found that habitat enhancement methods designed to provide the requirements of sensitive target biota consistently increased the richness and abundance of both rare and common species, with 10-fold to greater than 100-fold more rare species per sample area than generalized conventional conservation measures. Furthermore, targeting landscapes of high species richness amplified beneficial effects on the least mobile taxa: plants and bees. Our results provide the first unequivocal support for a national wildlife-friendly farming policy and suggest that this approach should be implemented much more extensively to address global biodiversity loss. However, to be effective, these conservation measures must be evidence-based, and developed using sound knowledge of the ecological requirements of key species. PMID:22675140

  4. Using SPOT-5 images in rice farming for detecting BPH (Brown Plant Hopper)

    NASA Astrophysics Data System (ADS)

    Ghobadifar, F.; Wayayok, A.; Shattri, M.; Shafri, H.

    2014-06-01

    Infestation of rice plant-hopper such as Brown Plant Hopper (BPH) (Nilaparvata lugens) is one of the most notable risk in rice yield in tropical areas especially in Asia. In order to use visible and infrared images to detect stress in rice production caused by BPH infestation, several remote sensing techniques have been developed. Initial recognition of pest infestation by means of remote sensing will spreads, for precision farming practice. To address this issue, detection of sheath blight in rice farming was examined by using SPOT-5 images. Specific image indices such as Normalized decrease food production costs, limit environmental hazards, and enhance natural pest control before the problem Normalized Difference Vegetation Index (NDVI), Standard difference indices (SDI) and Ratio Vegetation Index (RVI) were used for analyses using ENVI 4.8 and SPSS software. Results showed that all the indices to recognize infected plants are significant at α = 0.01. Examination of the association between the disease indices indicated that band 3 (near infrared) and band 4 (mid infrared) have a relatively high correlation. The selected indices declared better association for detecting healthy plants from diseased ones. Consequently, these sorts of indices especially NDVI could be valued as indicators for developing techniques for detecting the sheath blight of rice by using remote sensing. This infers that they are useful for crop disease detection but the spectral resolution is probably not sufficient to distinguish plants with light infections (low severity level). Using the index as an indicator can clarify the threshold for zoning the outbreaks. Quick assessment information is very useful in precision farming to practice site specific management such as pesticide application.

  5. On-farm conditions that compromise animal welfare that can be monitored at the slaughter plant.

    PubMed

    Grandin, Temple

    2017-10-01

    Handling and stunning at slaughter plants has greatly improved through the use of numerical scoring. The purpose of this paper is to encourage the use of numerical scoring systems at the slaughter plants to assess conditions that compromise welfare that occurred either during transport or on the farm. Some of the transport problems that can be assessed are bruises, death losses, and injured animals. Welfare issues that occurred on the farm that can be assessed at the abattoir are body condition, lameness, lesions, injuries, animal cleanliness and internal pathology. There are important welfare issues that cannot be assessed at slaughter. They are on-farm euthanasia methods, use of analgesics during surgeries, and the type of animal housing systems. Welfare evaluations at slaughter have the potential to greatly improve welfare. Copyright © 2017 The Author. Published by Elsevier Ltd.. All rights reserved.

  6. Prevalence of Salmonella in broilers at retail outlets, processing plants and farms in Malaysia.

    PubMed

    Rusul, G; Khair, J; Radu, S; Cheah, C T; Yassin, R M

    1996-12-01

    A study was conducted to estimate the prevalence of Salmonella among broilers retailed at wet-markets and processing plants. Litter and feed samples obtained from both broiler and breeder farms were also examined for Salmonella. A total of 158 out of 445 (35.5%) and 52 out of 104 (50.0%) broiler carcasses obtained from wet-markets and processing plants were contaminated with Salmonella, respectively. Salmonella was isolated from 14 out of 98 (14.3%) samples of intestinal content. Litter samples from broiler and breeder farms were positive for Salmonella, 8/40 (20%) and 2/10 (20%), respectively. Salmonella isolates (230) belonging to 15 different serovars were isolated. Predominant serovars were S. enteritidis, S. muenchen, S. kentucky and S. blockley.

  7. Synthesis, structural characterization and biological activity of two diastereomeric JA-Ile macrolactones.

    PubMed

    Jimenez-Aleman, Guillermo H; Machado, Ricardo A R; Görls, Helmar; Baldwin, Ian T; Boland, Wilhelm

    2015-06-07

    Jasmonates are phytohormones involved in a wide range of plant processes, including growth, development, senescence, and defense. Jasmonoyl-L-isoleucine (JA-Ile, 2), an amino acid conjugate of jasmonic acid (JA, 1), has been identified as a bioactive endogenous jasmonate. However, JA-Ile (2) analogues trigger different responses in the plant. ω-Hydroxylation of the pentenyl side chain leads to the inactive 12-OH-JA-Ile (3) acting as a “stop” signal. On the other hand, a lactone derivative of 12-OH-JA (5) (jasmine ketolactone, JKL) occurs in nature, although with no known biological function. Inspired by the chemical structure of JKL (6) and in order to further explore the potential biological activities of 12-modified JA-Ile derivatives, we synthesized two macrolactones (JA-Ile-lactones (4a) and (4b)) derived from 12-OH-JA-Ile (3). The biological activity of (4a) and (4b) was tested for their ability to elicit nicotine production, a well-known jasmonate dependent secondary metabolite. Both macrolactones showed strong biological activity, inducing nicotine accumulation to a similar extent as methyl jasmonate does in Nicotiana attenuata leaves. Surprisingly, the highest nicotine contents were found in plants treated with the JA-Ile-lactone (4b), which has (3S,7S) configuration at the cyclopentanone not known from natural jasmonates. Macrolactone (4a) is a valuable standard to explore for its occurrence in nature.

  8. A Critical Review of the Concept of Transgenic Plants: Insights into Pharmaceutical Biotechnology and Molecular Farming.

    PubMed

    Abiri, Rambod; Valdiani, Alireza; Maziah, Mahmood; Shaharuddin, Noor Azmi; Sahebi, Mahbod; Yusof, Zetty Norhana Balia; Atabaki, Narges; Talei, Daryush

    2016-01-01

    Using transgenic plants for the production of high-value recombinant proteins for industrial and clinical applications has become a promising alternative to using conventional bioproduction systems, such as bacteria, yeast, and cultured insect and animal cells. This novel system offers several advantages over conventional systems in terms of safety, scale, cost-effectiveness, and the ease of distribution and storage. Currently, plant systems are being utilised as recombinant bio-factories for the expression of various proteins, including potential vaccines and pharmaceuticals, through employing several adaptations of recombinant processes and utilizing the most suitable tools and strategies. The level of protein expression is a critical factor in plant molecular farming, and this level fluctuates according to the plant species and the organs involved. The production of recombinant native and engineered proteins is a complicated procedure that requires an inter- and multi-disciplinary effort involving a wide variety of scientific and technological disciplines, ranging from basic biotechnology, biochemistry, and cell biology to advanced production systems. This review considers important plant resources, affecting factors, and the recombinant-protein expression techniques relevant to the plant molecular farming process.

  9. Tightly-Coupled Plant-Soil Nitrogen Cycling: Comparison of Organic Farms across an Agricultural Landscape

    PubMed Central

    Bowles, Timothy M.; Hollander, Allan D.; Steenwerth, Kerri; Jackson, Louise E.

    2015-01-01

    How farming systems supply sufficient nitrogen (N) for high yields but with reduced N losses is a central challenge for reducing the tradeoffs often associated with N cycling in agriculture. Variability in soil organic matter and management of organic farms across an agricultural landscape may yield insights for improving N cycling and for evaluating novel indicators of N availability. We assessed yields, plant-soil N cycling, and root expression of N metabolism genes across a representative set of organic fields growing Roma-type tomatoes (Solanum lycopersicum L.) in an intensively-managed agricultural landscape in California, USA. The fields spanned a three-fold range of soil carbon (C) and N but had similar soil types, texture, and pH. Organic tomato yields ranged from 22.9 to 120.1 Mg ha-1 with a mean similar to the county average (86.1 Mg ha-1), which included mostly conventionally-grown tomatoes. Substantial variability in soil inorganic N concentrations, tomato N, and root gene expression indicated a range of possible tradeoffs between yields and potential for N losses across the fields. Fields showing evidence of tightly-coupled plant-soil N cycling, a desirable scenario in which high crop yields are supported by adequate N availability but low potential for N loss, had the highest total and labile soil C and N and received organic matter inputs with a range of N availability. In these fields, elevated expression of a key gene involved in root N assimilation, cytosolic glutamine synthetase GS1, confirmed that plant N assimilation was high even when inorganic N pools were low. Thus tightly-coupled N cycling occurred on several working organic farms. Novel combinations of N cycling indicators (i.e. inorganic N along with soil microbial activity and root gene expression for N assimilation) would support adaptive management for improved N cycling on organic as well as conventional farms, especially when plant-soil N cycling is rapid. PMID:26121264

  10. Tightly-Coupled Plant-Soil Nitrogen Cycling: Comparison of Organic Farms across an Agricultural Landscape.

    PubMed

    Bowles, Timothy M; Hollander, Allan D; Steenwerth, Kerri; Jackson, Louise E

    2015-01-01

    How farming systems supply sufficient nitrogen (N) for high yields but with reduced N losses is a central challenge for reducing the tradeoffs often associated with N cycling in agriculture. Variability in soil organic matter and management of organic farms across an agricultural landscape may yield insights for improving N cycling and for evaluating novel indicators of N availability. We assessed yields, plant-soil N cycling, and root expression of N metabolism genes across a representative set of organic fields growing Roma-type tomatoes (Solanum lycopersicum L.) in an intensively-managed agricultural landscape in California, USA. The fields spanned a three-fold range of soil carbon (C) and N but had similar soil types, texture, and pH. Organic tomato yields ranged from 22.9 to 120.1 Mg ha-1 with a mean similar to the county average (86.1 Mg ha-1), which included mostly conventionally-grown tomatoes. Substantial variability in soil inorganic N concentrations, tomato N, and root gene expression indicated a range of possible tradeoffs between yields and potential for N losses across the fields. Fields showing evidence of tightly-coupled plant-soil N cycling, a desirable scenario in which high crop yields are supported by adequate N availability but low potential for N loss, had the highest total and labile soil C and N and received organic matter inputs with a range of N availability. In these fields, elevated expression of a key gene involved in root N assimilation, cytosolic glutamine synthetase GS1, confirmed that plant N assimilation was high even when inorganic N pools were low. Thus tightly-coupled N cycling occurred on several working organic farms. Novel combinations of N cycling indicators (i.e. inorganic N along with soil microbial activity and root gene expression for N assimilation) would support adaptive management for improved N cycling on organic as well as conventional farms, especially when plant-soil N cycling is rapid.

  11. Disarming the jasmonate-dependent plant defense makes nonhost Arabidopsis plants accessible to the American serpentine leafminer.

    PubMed

    Abe, Hiroshi; Tateishi, Ken; Seo, Shigemi; Kugimiya, Soichi; Hirai, Masami Yokota; Sawada, Yuji; Murata, Yoshiyuki; Yara, Kaori; Shimoda, Takeshi; Kobayashi, Masatomo

    2013-11-01

    Here, we analyzed the interaction between Arabidopsis (Arabidopsis thaliana) and the American serpentine leafminer (Liriomyza trifolii), an important and intractable herbivore of many cultivated plants. We examined the role of the immunity-related plant hormone jasmonate (JA) in the plant response and resistance to leafminer feeding to determine whether JA affects host suitability for leafminers. The expression of marker genes for the JA-dependent plant defense was induced by leafminer feeding on Arabidopsis wild-type plants. Analyses of JA-insensitive coi1-1 mutants suggested the importance of JA in the plant response to leafminer feeding. The JA content of wild-type plants significantly increased after leafminer feeding. Moreover, coi1-1 mutants showed lower feeding resistance against leafminer attack than did wild-type plants. The number of feeding scars caused by inoculated adult leafminers in JA-insensitive coi1-1 mutants was higher than that in wild-type plants. In addition, adults of the following generation appeared only from coi1-1 mutants and not from wild-type plants, suggesting that the loss of the JA-dependent plant defense converted nonhost plants to accessible host plants. Interestingly, the glucosinolate-myrosinase defense system may play at most a minor role in this conversion, indicating that this major antiherbivore defense of Brassica species plants probably does not have a major function in plant resistance to leafminer. Application of JA to wild-type plants before leafminer feeding enhanced feeding resistance in Chinese cabbage (Brassica rapa), tomato (Solanum lycopersicum), and garland chrysanthemum (Chrysanthemum coronarium). Our results indicate that JA plays an important role in the plant response and resistance to leafminers and, in so doing, affects host plant suitability for leafminers.

  12. Enumeration of Salmonella and Campylobacter spp. in environmental farm samples and processing plant carcass rinses from commercial broiler chicken flocks.

    PubMed

    Berghaus, Roy D; Thayer, Stephan G; Law, Bibiana F; Mild, Rita M; Hofacre, Charles L; Singer, Randall S

    2013-07-01

    A prospective cohort study was performed to evaluate the prevalences and loads of Salmonella and Campylobacter spp. in farm and processing plant samples collected from 55 commercial broiler chicken flocks. Environmental samples were collected from broiler houses within 48 h before slaughter, and carcass rinses were performed on birds from the same flocks at 4 different stages of processing. Salmonella was detected in farm samples of 50 (90.9%) flocks and in processing samples of 52 (94.5%) flocks. Campylobacter was detected in farm samples of 35 (63.6%) flocks and in processing samples of 48 (87.3%) flocks. There was a significant positive relationship between environmental farm samples and processing plant carcass rinses with respect to both Salmonella and Campylobacter prevalences and loads. Campylobacter loads were significantly higher than Salmonella loads, and the correlations between samples collected from the same flocks were higher for Campylobacter than they were for Salmonella. Boot socks were the most sensitive sample type for detection of Salmonella on the farm, whereas litter samples had the strongest association with Salmonella loads in pre- and postchill carcass rinses. Boot socks, drag swabs, and fecal samples all had similar sensitivities for detecting Campylobacter on the farm, and all were more strongly associated with Campylobacter loads in carcass rinses than were litter samples. Farm samples explained a greater proportion of the variability in carcass rinse prevalences and loads for Campylobacter than they did for Salmonella. Salmonella and Campylobacter prevalences and loads both decreased significantly as birds progressed through the processing plant.

  13. Enumeration of Salmonella and Campylobacter spp. in Environmental Farm Samples and Processing Plant Carcass Rinses from Commercial Broiler Chicken Flocks

    PubMed Central

    Thayer, Stephan G.; Law, Bibiana F.; Mild, Rita M.; Hofacre, Charles L.; Singer, Randall S.

    2013-01-01

    A prospective cohort study was performed to evaluate the prevalences and loads of Salmonella and Campylobacter spp. in farm and processing plant samples collected from 55 commercial broiler chicken flocks. Environmental samples were collected from broiler houses within 48 h before slaughter, and carcass rinses were performed on birds from the same flocks at 4 different stages of processing. Salmonella was detected in farm samples of 50 (90.9%) flocks and in processing samples of 52 (94.5%) flocks. Campylobacter was detected in farm samples of 35 (63.6%) flocks and in processing samples of 48 (87.3%) flocks. There was a significant positive relationship between environmental farm samples and processing plant carcass rinses with respect to both Salmonella and Campylobacter prevalences and loads. Campylobacter loads were significantly higher than Salmonella loads, and the correlations between samples collected from the same flocks were higher for Campylobacter than they were for Salmonella. Boot socks were the most sensitive sample type for detection of Salmonella on the farm, whereas litter samples had the strongest association with Salmonella loads in pre- and postchill carcass rinses. Boot socks, drag swabs, and fecal samples all had similar sensitivities for detecting Campylobacter on the farm, and all were more strongly associated with Campylobacter loads in carcass rinses than were litter samples. Farm samples explained a greater proportion of the variability in carcass rinse prevalences and loads for Campylobacter than they did for Salmonella. Salmonella and Campylobacter prevalences and loads both decreased significantly as birds progressed through the processing plant. PMID:23624481

  14. Disarming the Jasmonate-Dependent Plant Defense Makes Nonhost Arabidopsis Plants Accessible to the American Serpentine Leafminer1

    PubMed Central

    Abe, Hiroshi; Tateishi, Ken; Seo, Shigemi; Kugimiya, Soichi; Hirai, Masami Yokota; Sawada, Yuji; Murata, Yoshiyuki; Yara, Kaori; Shimoda, Takeshi; Kobayashi, Masatomo

    2013-01-01

    Here, we analyzed the interaction between Arabidopsis (Arabidopsis thaliana) and the American serpentine leafminer (Liriomyza trifolii), an important and intractable herbivore of many cultivated plants. We examined the role of the immunity-related plant hormone jasmonate (JA) in the plant response and resistance to leafminer feeding to determine whether JA affects host suitability for leafminers. The expression of marker genes for the JA-dependent plant defense was induced by leafminer feeding on Arabidopsis wild-type plants. Analyses of JA-insensitive coi1-1 mutants suggested the importance of JA in the plant response to leafminer feeding. The JA content of wild-type plants significantly increased after leafminer feeding. Moreover, coi1-1 mutants showed lower feeding resistance against leafminer attack than did wild-type plants. The number of feeding scars caused by inoculated adult leafminers in JA-insensitive coi1-1 mutants was higher than that in wild-type plants. In addition, adults of the following generation appeared only from coi1-1 mutants and not from wild-type plants, suggesting that the loss of the JA-dependent plant defense converted nonhost plants to accessible host plants. Interestingly, the glucosinolate-myrosinase defense system may play at most a minor role in this conversion, indicating that this major antiherbivore defense of Brassica species plants probably does not have a major function in plant resistance to leafminer. Application of JA to wild-type plants before leafminer feeding enhanced feeding resistance in Chinese cabbage (Brassica rapa), tomato (Solanum lycopersicum), and garland chrysanthemum (Chrysanthemum coronarium). Our results indicate that JA plays an important role in the plant response and resistance to leafminers and, in so doing, affects host plant suitability for leafminers. PMID:24022267

  15. Planning of dairy farm and dairy plant based ecotourism

    NASA Astrophysics Data System (ADS)

    Sarnyoto, A. S.; Tama, I. P.; Tantrika, C. F. M.

    2017-06-01

    One of a dairy production company producing pasteurized milk and yoghurt drink, whose brand has widely known in East Java, has a factory plant in Batu City, one of tourism destinations in Indonesia. Behind the factory plant, there is a vacant land with an estimated total area of 2.3 ha and a vacant cowshed which had not been used for cattle ranching. Because of that, the company planned to develop the vacant land as a dairy farm and plant based ecotourism. In addition, dairy farm and dairy plant based tourism attractions are still rarely found in Batu. Thus, the first aim of this study was to analyse the potencies of the company that related to future plans of ecotourism built. The second aim was to set up the strategies that can be done in order to actualize the ecotourism project. The next aim was to plan the ecotourism, especially the facilities planning and the facilities arrangement on the vacant land. Strategic management approach was used to analyse the potencies and to determine the strategies. To select the proper facilities, tourists were asked to give appraisal by using questionnaire. Appraisal result was mapped onto four quadrants spatial map to see advantages and shortcomings of each facility along with choosing the right facilities to be built. Those facilities and tourist activities were compared with ecotourism criteria to make sure that the facilities were appropriate to provide not only entertainment but also ecotourism function. To arrange the chosen facilities, the step in Systematic Layout Planning were conducted to generate a propose layout of facilities arrangement. Based on potencies analysis, in Internal-External matrix, the company current position was on quadrant 2 (grow and build), with the most appropriate strategy was intensive or integrative. The proposed strategies were to build the new infrastructure, to renovate cowshed, and to add new tourism facilities on the land. There were 11 selected facilities based on MDS. Moreover, based

  16. Urtica dioica (Stinging Nettle): A Neglected Plant With Emerging Growth Promoter/Immunostimulant Properties for Farmed Fish.

    PubMed

    De Vico, Gionata; Guida, Vincenzo; Carella, Francesca

    2018-01-01

    Urtica dioica (stinging nettle), is a perennial plant belonging to the family of Urticaceae , genus Urtica . Despite the use of nettle in folk veterinary medicine is well documented, U. dioica is today an underestimated and frequently neglected plant, considered by the contemporary agriculture as a weed to be eliminated. This mini review focus on very recent studies on dietary administration of U. dioica , both as a single herb or in combination with other herbs, to enhance growth and stimulate farmed fish immunity, thus enabling the fish to be more resistant against bacterial infections. Such an emerging feature, together with cost-effectiveness, adequate availability, and easy processing of nettle, could make this herb an excellent, inexpensive and widely used dietary supplement on intensive fish farms.

  17. Urtica dioica (Stinging Nettle): A Neglected Plant With Emerging Growth Promoter/Immunostimulant Properties for Farmed Fish

    PubMed Central

    De Vico, Gionata; Guida, Vincenzo; Carella, Francesca

    2018-01-01

    Urtica dioica (stinging nettle), is a perennial plant belonging to the family of Urticaceae, genus Urtica. Despite the use of nettle in folk veterinary medicine is well documented, U. dioica is today an underestimated and frequently neglected plant, considered by the contemporary agriculture as a weed to be eliminated. This mini review focus on very recent studies on dietary administration of U. dioica, both as a single herb or in combination with other herbs, to enhance growth and stimulate farmed fish immunity, thus enabling the fish to be more resistant against bacterial infections. Such an emerging feature, together with cost-effectiveness, adequate availability, and easy processing of nettle, could make this herb an excellent, inexpensive and widely used dietary supplement on intensive fish farms. PMID:29632497

  18. Parasitism by Cuscuta pentagona sequentially induces JA and SA defence pathways in tomato.

    PubMed

    Runyon, Justin B; Mescher, Mark C; Felton, Gary W; De Moraes, Consuelo M

    2010-02-01

    While plant responses to herbivores and pathogens are well characterized, responses to attack by other plants remain largely unexplored. We measured phytohormones and C(18) fatty acids in tomato attacked by the parasitic plant Cuscuta pentagona, and used transgenic and mutant plants to explore the roles of the defence-related phytohormones salicylic acid (SA) and jasmonic acid (JA). Parasite attachment to 10-day-old tomato plants elicited few biochemical changes, but a second attachment 10 d later elicited a 60-fold increase in JA, a 30-fold increase in SA and a hypersensitive-like response (HLR). Host age also influenced the response: neither Cuscuta seedlings nor established vines elicited a HLR in 10-day-old hosts, but both did in 20-day-old hosts. Parasites grew larger on hosts deficient in SA (NahG) or insensitive to JA [jasmonic acid-insensitive1 (jai1)], suggesting that both phytohormones mediate effective defences. Moreover, amounts of JA peaked 12 h before SA, indicating that defences may be coordinated via sequential induction of these hormones. Parasitism also induced increases in free linolenic and linoleic acids and abscisic acid. These findings provide the first documentation of plant hormonal signalling induced by a parasitic plant and show that tomato responses to C. pentagona display characteristics similar to both herbivore- and pathogen-induced responses.

  19. Jasmonate-triggered plant immunity.

    PubMed

    Campos, Marcelo L; Kang, Jin-Ho; Howe, Gregg A

    2014-07-01

    The plant hormone jasmonate (JA) exerts direct control over the production of chemical defense compounds that confer resistance to a remarkable spectrum of plant-associated organisms, ranging from microbial pathogens to vertebrate herbivores. The underlying mechanism of JA-triggered immunity (JATI) can be conceptualized as a multi-stage signal transduction cascade involving: i) pattern recognition receptors (PRRs) that couple the perception of danger signals to rapid synthesis of bioactive JA; ii) an evolutionarily conserved JA signaling module that links fluctuating JA levels to changes in the abundance of transcriptional repressor proteins; and iii) activation (de-repression) of transcription factors that orchestrate the expression of myriad chemical and morphological defense traits. Multiple negative feedback loops act in concert to restrain the duration and amplitude of defense responses, presumably to mitigate potential fitness costs of JATI. The convergence of diverse plant- and non-plant-derived signals on the core JA module indicates that JATI is a general response to perceived danger. However, the modular structure of JATI may accommodate attacker-specific defense responses through evolutionary innovation of PRRs (inputs) and defense traits (outputs). The efficacy of JATI as a defense strategy is highlighted by its capacity to shape natural populations of plant attackers, as well as the propensity of plant-associated organisms to subvert or otherwise manipulate JA signaling. As both a cellular hub for integrating informational cues from the environment and a common target of pathogen effectors, the core JA module provides a focal point for understanding immune system networks and the evolution of chemical diversity in the plant kingdom.

  20. Desert farming benefits from microbial potential in arid soils and promotes diversity and plant health.

    PubMed

    Köberl, Martina; Müller, Henry; Ramadan, Elshahat M; Berg, Gabriele

    2011-01-01

    To convert deserts into arable, green landscapes is a global vision, and desert farming is a strong growing area of agriculture world-wide. However, its effect on diversity of soil microbial communities, which are responsible for important ecosystem services like plant health, is still not known. We studied the impact of long-term agriculture on desert soil in one of the most prominent examples for organic desert farming in Sekem (Egypt). Using a polyphasic methodological approach to analyse microbial communities in soil as well as associated with cultivated plants, drastic effects caused by 30 years of agriculture were detected. Analysing bacterial fingerprints, we found statistically significant differences between agricultural and native desert soil of about 60%. A pyrosequencing-based analysis of the 16S rRNA gene regions showed higher diversity in agricultural than in desert soil (Shannon diversity indices: 11.21/7.90), and displayed structural differences. The proportion of Firmicutes in field soil was significantly higher (37%) than in the desert (11%). Bacillus and Paenibacillus play the key role: they represented 96% of the antagonists towards phytopathogens, and identical 16S rRNA sequences in the amplicon library and for isolates were detected. The proportion of antagonistic strains was doubled in field in comparison to desert soil (21.6%/12.4%); disease-suppressive bacteria were especially enriched in plant roots. On the opposite, several extremophilic bacterial groups, e.g., Acidimicrobium, Rubellimicrobium and Deinococcus-Thermus, disappeared from soil after agricultural use. The N-fixing Herbaspirillum group only occurred in desert soil. Soil bacterial communities were strongly driven by the a-biotic factors water supply and pH. After long-term farming, a drastic shift in the bacterial communities in desert soil was observed. Bacterial communities in agricultural soil showed a higher diversity and a better ecosystem function for plant health but a

  1. Desert Farming Benefits from Microbial Potential in Arid Soils and Promotes Diversity and Plant Health

    PubMed Central

    Köberl, Martina; Müller, Henry; Ramadan, Elshahat M.; Berg, Gabriele

    2011-01-01

    Background To convert deserts into arable, green landscapes is a global vision, and desert farming is a strong growing area of agriculture world-wide. However, its effect on diversity of soil microbial communities, which are responsible for important ecosystem services like plant health, is still not known. Methodology/Principal Findings We studied the impact of long-term agriculture on desert soil in one of the most prominent examples for organic desert farming in Sekem (Egypt). Using a polyphasic methodological approach to analyse microbial communities in soil as well as associated with cultivated plants, drastic effects caused by 30 years of agriculture were detected. Analysing bacterial fingerprints, we found statistically significant differences between agricultural and native desert soil of about 60%. A pyrosequencing-based analysis of the 16S rRNA gene regions showed higher diversity in agricultural than in desert soil (Shannon diversity indices: 11.21/7.90), and displayed structural differences. The proportion of Firmicutes in field soil was significantly higher (37%) than in the desert (11%). Bacillus and Paenibacillus play the key role: they represented 96% of the antagonists towards phytopathogens, and identical 16S rRNA sequences in the amplicon library and for isolates were detected. The proportion of antagonistic strains was doubled in field in comparison to desert soil (21.6%/12.4%); disease-suppressive bacteria were especially enriched in plant roots. On the opposite, several extremophilic bacterial groups, e.g., Acidimicrobium, Rubellimicrobium and Deinococcus-Thermus, disappeared from soil after agricultural use. The N-fixing Herbaspirillum group only occurred in desert soil. Soil bacterial communities were strongly driven by the a-biotic factors water supply and pH. Conclusions/Significance After long-term farming, a drastic shift in the bacterial communities in desert soil was observed. Bacterial communities in agricultural soil showed a higher

  2. Push-pull farming systems.

    PubMed

    Pickett, John A; Woodcock, Christine M; Midega, Charles A O; Khan, Zeyaur R

    2014-04-01

    Farming systems for pest control, based on the stimulo-deterrent diversionary strategy or push-pull system, have become an important target for sustainable intensification of food production. A prominent example is push-pull developed in sub-Saharan Africa using a combination of companion plants delivering semiochemicals, as plant secondary metabolites, for smallholder farming cereal production, initially against lepidopterous stem borers. Opportunities are being developed for other regions and farming ecosystems. New semiochemical tools and delivery systems, including GM, are being incorporated to exploit further opportunities for mainstream arable farming systems. By delivering the push and pull effects as secondary metabolites, for example, (E)-4,8-dimethyl-1,3,7-nonatriene repelling pests and attracting beneficial insects, problems of high volatility and instability are overcome and compounds are produced when and where required. Copyright © 2013 The Authors. Published by Elsevier Ltd.. All rights reserved.

  3. Organic Farming and Landscape Structure: Effects on Insect-Pollinated Plant Diversity in Intensively Managed Grasslands

    PubMed Central

    Power, Eileen F.; Kelly, Daniel L.; Stout, Jane C.

    2012-01-01

    Parallel declines in insect-pollinated plants and their pollinators have been reported as a result of agricultural intensification. Intensive arable plant communities have previously been shown to contain higher proportions of self-pollinated plants compared to natural or semi-natural plant communities. Though intensive grasslands are widespread, it is not known whether they show similar patterns to arable systems nor whether local and/or landscape factors are influential. We investigated plant community composition in 10 pairs of organic and conventional dairy farms across Ireland in relation to the local and landscape context. Relationships between plant groups and local factors (farming system, position in field and soil parameters) and landscape factors (e.g. landscape complexity) were investigated. The percentage cover of unimproved grassland was used as an inverse predictor of landscape complexity, as it was negatively correlated with habitat-type diversity. Intensive grasslands (organic and conventional) contained more insect-pollinated forbs than non-insect pollinated forbs. Organic field centres contained more insect-pollinated forbs than conventional field centres. Insect-pollinated forb richness in field edges (but not field centres) increased with increasing landscape complexity (% unimproved grassland) within 1, 3, 4 and 5km radii around sites, whereas non-insect pollinated forb richness was unrelated to landscape complexity. Pollination systems within intensive grassland communities may be different from those in arable systems. Our results indicate that organic management increases plant richness in field centres, but that landscape complexity exerts strong influences in both organic and conventional field edges. Insect-pollinated forb richness, unlike that for non-insect pollinated forbs, showed positive relationships to landscape complexity reflecting what has been documented for bees and other pollinators. The insect-pollinated forbs, their

  4. Pioneer farming in southeast Europe during the early sixth millennium BC: Climate-related adaptations in the exploitation of plants and animals

    PubMed Central

    De Cupere, Bea; Ethier, Jonathan; Marinova, Elena

    2018-01-01

    The Old World farming system arose in the semi-arid Mediterranean environments of southwest Asia. Pioneer farmers settling the interior of the Balkans by the early sixth millennium BC were among the first to introduce southwest Asian-style cultivation and herding into areas with increasingly continental temperate conditions. Previous research has shown that the bioarchaeological assemblages from early farming sites in southeast Europe vary in their proportions of plant and animal taxa, but the relationship between taxonomic variation and climate has remained poorly understood. To uncover associations between multiple species and environmental factors simultaneously, we explored a dataset including altitude, five bioclimatic and 30 bioarchaeological variables (plant and animal taxa) for 57 of the earliest farming sites in southeast Europe using Canonical Correspondence Analysis (CCA). An extension of correspondence analysis, CCA is widely used in applied ecology to answer similar questions of species-environment relationships, but has not been previously applied in prehistoric archaeology to explore taxonomic and climatic variables in conjunction. The analyses reveal that the changes in plant and animal exploitation which occurred with the northward dispersal of farmers, crops and livestock correlate with south-north climate gradients, and emphasize the importance of adaptations in the animal domain for the initial establishment of farming beyond the Mediterranean areas. PMID:29775469

  5. Advances in greenhouse automation and controlled environment agriculture: A transition to plant factories and urban farming

    USDA-ARS?s Scientific Manuscript database

    Greenhouse cultivation has evolved from simple covered rows of open-fields crops to highly sophisticated controlled environment agriculture (CEA) facilities that projected the image of plant factories for urban farming. The advances and improvements in CEA have promoted the scientific solutions for ...

  6. The tomato res mutant which accumulates JA in roots in non-stressed conditions restores cell structure alterations under salinity.

    PubMed

    Garcia-Abellan, José O; Fernandez-Garcia, Nieves; Lopez-Berenguer, Carmen; Egea, Isabel; Flores, Francisco B; Angosto, Trinidad; Capel, Juan; Lozano, Rafael; Pineda, Benito; Moreno, Vicente; Olmos, Enrique; Bolarin, Maria C

    2015-11-01

    Jasmonic acid (JA) regulates a wide spectrum of plant biological processes, from plant development to stress defense responses. The role of JA in plant response to salt stress is scarcely known, and even less known is the specific response in root, the main plant organ responsible for ionic uptake and transport to the shoot. Here we report the characterization of the first tomato (Solanum lycopersicum) mutant, named res (restored cell structure by salinity), that accumulates JA in roots prior to exposure to stress. The res tomato mutant presented remarkable growth inhibition and displayed important morphological alterations and cellular disorganization in roots and leaves under control conditions, while these alterations disappeared when the res mutant plants were grown under salt stress. Reciprocal grafting between res and wild type (WT) (tomato cv. Moneymaker) indicated that the main organ responsible for the development of alterations was the root. The JA-signaling pathway is activated in res roots prior to stress, with transcripts levels being even higher in control condition than in salinity. Future studies on this mutant will provide significant advances in the knowledge of JA role in root in salt-stress tolerance response, as well as in the energy trade-off between plant growth and response to stress. © 2015 Scandinavian Plant Physiology Society.

  7. Ethnoveterinary plants and practices used for ecto-parasite control in semi-arid smallholder farming areas of Zimbabwe.

    PubMed

    Nyahangare, Emmanuel Tendai; Mvumi, Brighton Marimanzi; Mutibvu, Tonderai

    2015-04-30

    The inclusion of traditional plant-based ecto-parasite control methods in primary health care of livestock is increasingly becoming an important intervention for improving livestock productivity in resource-challenged smallholder farming areas. In this study, commonly used plants used for the control of cattle ticks and other pests were identified through a survey in four semi-arid districts of Zimbabwe. A standard structured questionnaire with details of demographics, socioeconomic status of households, livestock parasites, control practices and list of ethnoveterinary plants used was used to interview 233 knowledgeable smallholder farmers in four districts. Focus group discussions with community members further provided insights on how the plants were being used and other issues surrounding ecto-parasite control and indigenous knowledge systems in the study areas. The older generation (>40 years) of the respondents were knowledgeable about ethnoveterinary plants and practices. Overall, 51 plant species were reportedly effective against cattle ticks and other livestock parasites. The most frequently mentioned plants were in descending order, Cissus quadrangularis (30.1%), Lippia javanica (19.6%), Psydrax livida (14.9%) and Aloe sp (14.9%). Most of the plant materials were prepared by crushing and soaking in water and spraying the extract on animals. Despite the knowledge of these useful pesticidal plants, the preferred animal health care for cattle and other highly ranked livestock species is still the use of commercial acaricides. Cattle dipping services were reported sporadic by 48% of the respondents. Traditional knowledge and plants are considered only as an alternative in the absence of conventional synthetic products. Livestock farming communities know of plant species used for livestock ecto-parasite control. The plant species are mostly used to complement commercial products. More work, is required to confirm the acaricidal properties claimed by the

  8. UV-C-Induced alleviation of transcriptional gene silencing through plant-plant communication: Key roles of jasmonic acid and salicylic acid pathways.

    PubMed

    Xu, Wei; Wang, Ting; Xu, Shaoxin; Li, Fanghua; Deng, Chenguang; Wu, Lijun; Wu, Yuejin; Bian, Po

    2016-08-01

    Plant stress responses at the epigenetic level are expected to allow more permanent changes of gene expression and potentially long-term adaptation. While it has been reported that plants subjected to adverse environments initiate various stress responses in their neighboring plants, little is known regarding epigenetic responses to external stresses mediated by plant-plant communication. In this study, we show that DNA repetitive elements of Arabidopsis thaliana, whose expression is inhibited epigenetically by transcriptional gene silencing (TGS) mechanism, are activated by UV-C irradiation through airborne plant-plant and plant-plant-plant communications, accompanied by DNA demethylation at CHH sites. Moreover, the TGS is alleviated by direct treatments with exogenous methyl jasmonate (MeJA) and methyl salicylate (MeSA). Further, the plant-plant and plant-plant-plant communications are blocked by mutations in the biosynthesis or signaling of jasmonic acid (JA) or salicylic acid (SA), indicating that JA and SA pathways are involved in the interplant communication for epigenetic responses. For the plant-plant-plant communication, stress cues are relayed to the last set of receiver plants by promoting the production of JA and SA signals in relaying plants, which exhibit upregulated expression of genes for JA and SA biosynthesis and enhanced emanation of MeJA and MeSA. Copyright © 2016 Elsevier B.V. All rights reserved.

  9. Two bHLH-type transcription factors, JA-ASSOCIATED MYC2-LIKE2 and JAM3, are transcriptional repressors and affect male fertility

    PubMed Central

    Nakata, Masaru; Ohme-Takagi, Masaru

    2013-01-01

    The jasmonate (JA) plant hormones regulate responses to biotic and abiotic stress and aspects of plant development, including male fertility in Arabidopsis thaliana. The bHLH-type transcription factor JA-ASSOCIATED MYC2-LIKE1 (JAM1) negatively regulates JA signaling and gain-of-function JAM1 transgenic plants have impaired JA-mediated male fertility. Here we report that JAM2 and JAM3, 2 bHLHs closely related to JAM1, also act as transcriptional repressors. Moreover, overexpression of JAM2 and JAM3 also results in reduced male fertility. These results suggest that JAM1, JAM2, and JAM3 act redundantly as negative regulators of JA-mediated male fertility. PMID:24056034

  10. Endogenous Bioactive Jasmonate Is Composed of a Set of (+)-7-iso-JA-Amino Acid Conjugates1

    PubMed Central

    Li, Suhua; Li, Yuwen; Chen, Juan; Yang, Mai; Tong, Jianhua; Xiao, Langtao; Nan, Fajun; Xie, Daoxin

    2016-01-01

    Jasmonates (JAs) regulate a wide range of plant defense and development processes. The bioactive JA is perceived by its receptor COI1 to trigger the degradation of JASMONATE ZIM-DOMAIN (JAZ) proteins and subsequently derepress the JAZ-repressed transcription factors for activation of expression of JA-responsive genes. So far, (+)-7-iso-JA-l-Ile has been the only identified endogenous bioactive JA molecule. Here, we designed coronafacic acid (CFA) conjugates with all the amino acids (CFA-AA) to mimic the JA amino acid conjugates, and revealed that (+)-7-iso-JA-Leu, (+)-7-iso-JA-Val, (+)-7-iso-JA-Met, and (+)-7-iso-JA-Ala are new endogenous bioactive JA molecules. Furthermore, our studies uncover the general characteristics for all the bioactive JA molecules, and provide a new strategy to synthetically generate novel active JA molecules. PMID:27756820

  11. Allelopathy relationship between plants and their use in organic farming

    NASA Astrophysics Data System (ADS)

    Marian, M.; Voşgan, Z.; Mare Roşca, O.; Mihalescu, L.

    2017-05-01

    Allelopathy is a process still little studied in the plant world, if we refer to the diversity of biochemical compounds, through which plants can interact with each other, with fungi or bacteria. Biochemical "dialogue" between organisms may have stimulatory or inhibitory effects, contributing to numerical setting of the populations, the assertion of some species over others, the establishment and strengthen of plant communities. Practically, the allelopathy can be exploited in organic farming in understanding and identifying compatibility between species, to finding natural substances with herbicide potential. In experiments conducted, diluted extracts of Taraxacum officinale and Cirsium vulgare strongly inhibited the germination and growth of corn and beans, while Hedera helix produced the same effect, but at higher concentrations of the extract. Humulus lupulus extract has a stimulating effect on the two species and it is possible to use as natural fertilizer. Extracts of Chenopodium album works as stimulator for Beta vulgaris, and potent inhibitor for Triticum aestivum. Agropyron repens is an aggressive competitor for Lycopersicon esculentum and Capsicum annuum, producing a strong inhibition. Juglans regia extracts and especially those of Satureja hortensis, can be used as natural herbicides for up delay germination and growth suppression for the species: Echinochloa crus-galli and Setaria glauca.

  12. Citrus leprosis virus C Infection Results in Hypersensitive-Like Response, Suppression of the JA/ET Plant Defense Pathway and Promotion of the Colonization of Its Mite Vector

    PubMed Central

    Arena, Gabriella D.; Ramos-González, Pedro L.; Nunes, Maria A.; Ribeiro-Alves, Marcelo; Camargo, Luis E. A.; Kitajima, Elliot W.; Machado, Marcos A.; Freitas-Astúa, Juliana

    2016-01-01

    Leprosis is a serious disease of citrus caused by Citrus leprosis virus C (CiLV-C, genus Cilevirus) whose transmission is mediated by false spider mites of the genus Brevipalpus. CiLV-C infection does not systemically spread in any of its known host plants, thus remaining restricted to local lesions around the feeding sites of viruliferous mites. To get insight into this unusual pathosystem, we evaluated the expression profiles of genes involved in defense mechanisms of Arabidopsis thaliana and Citrus sinensis upon infestation with non-viruliferous and viruliferous mites by using reverse-transcription qPCR. These results were analyzed together with the production of reactive oxygen species (ROS) and the appearance of dead cells as assessed by histochemical assays. After interaction with non-viruliferous mites, plants locally accumulated ROS and triggered the salicylic acid (SA) and jasmonate/ethylene (JA/ET) pathways. ERF branch of the JA/ET pathways was highly activated. In contrast, JA pathway genes were markedly suppressed upon the CiLV-C infection mediated by viruliferous mites. Viral infection also intensified the ROS burst and cell death, and enhanced the expression of genes involved in the RNA silencing mechanism and SA pathway. After 13 days of infestation of two sets of Arabidopsis plants with non-viruliferous and viruliferous mites, the number of mites in the CiLV-C infected Arabidopsis plants was significantly higher than in those infested with the non-viruliferous ones. Oviposition of the viruliferous mites occurred preferentially in the CiLV-C infected leaves. Based on these results, we postulated the first model of plant/Brevipalpus mite/cilevirus interaction in which cells surrounding the feeding sites of viruliferous mites typify the outcome of a hypersensitive-like response, whereas viral infection induces changes in the behavior of its vector. PMID:27933078

  13. Molecular farming of human cytokines and blood products from plants: challenges in biosynthesis and detection of plant-produced recombinant proteins.

    PubMed

    da Cunha, Nicolau B; Vianna, Giovanni R; da Almeida Lima, Thaina; Rech, Elíbio

    2014-01-01

    Plants have emerged as an attractive alternative to the traditional mammalian cell cultures or microbial cell-based systems system for the production of valuable recombinant proteins. Through recombinant DNA technology, plants can be engineered to produce large quantities of pharmaceuticals and industrial proteins of high quality at low costs. The recombinant production, by transgenic plants, of therapeutic proteins normally present in human plasma, such as cytokines, coagulation factors, anticoagulants, and immunoglobulins, represents a response to the ongoing challenges in meeting the demand for therapeutic proteins to treat serious inherited or acquired bleeding and immunological diseases. As the clinical utilization of fractionated plasma molecules is limited by high production costs, using recombinant biopharmaceuticals derived from plants represents a feasible alternative to provide efficient treatment. Plant-derived pharmaceuticals also reduce the potential risks to patients of infection with pathogens or unwanted immune responses due to immunogenic antigens. In this review, we summarize the recent advances in molecular farming of cytokines. We also examine the technological basis, upcoming challenges, and perspectives for the biosynthesis and detection of these molecules in different plant production platforms. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  14. Parasitism by Cuscuta pentagona attenuates host plant defenses against insect herbivores.

    PubMed

    Runyon, Justin B; Mescher, Mark C; De Moraes, Consuelo M

    2008-03-01

    Considerable research has examined plant responses to concurrent attack by herbivores and pathogens, but the effects of attack by parasitic plants, another important class of plant-feeding organisms, on plant defenses against other enemies has not been explored. We investigated how attack by the parasitic plant Cuscuta pentagona impacted tomato (Solanum lycopersicum) defenses against the chewing insect beet armyworm (Spodoptera exigua; BAW). In response to insect feeding, C. pentagona-infested (parasitized) tomato plants produced only one-third of the antiherbivore phytohormone jasmonic acid (JA) produced by unparasitized plants. Similarly, parasitized tomato, in contrast to unparasitized plants, failed to emit herbivore-induced volatiles after 3 d of BAW feeding. Although parasitism impaired antiherbivore defenses, BAW growth was slower on parasitized tomato leaves. Vines of C. pentagona did not translocate JA from BAW-infested plants: amounts of JA in parasite vines grown on caterpillar-fed and control plants were similar. Parasitized plants generally contained more salicylic acid (SA), which can inhibit JA in some systems. Parasitized mutant (NahG) tomato plants deficient in SA produced more JA in response to insect feeding than parasitized wild-type plants, further suggesting cross talk between the SA and JA defense signaling pathways. However, JA induction by BAW was still reduced in parasitized compared to unparasitized NahG, implying that other factors must be involved. We found that parasitized plants were capable of producing induced volatiles when experimentally treated with JA, indicating that resource depletion by the parasite does not fully explain the observed attenuation of volatile response to herbivore feeding. Collectively, these findings show that parasitic plants can have important consequences for host plant defense against herbivores.

  15. Mass loading of typical artificial sweeteners in a pig farm and their dissipation and uptake by plants in neighboring farmland.

    PubMed

    Ma, Ling; Liu, Yarui; Xu, Jiayao; Sun, Hongwen; Chen, Hao; Yao, Yiming; Zhang, Peng; Shen, Fengju; Alder, Aldredo C

    2017-12-15

    Pig farm is an important potential source for artificial sweeteners (ASs) in the environment due to their wide use as additives in pig feed. The objective of this study was to evaluate the fate of typical ASs in pig farm and neighboring farmland. For this purpose, the levels of four typical artificial ASs, i.e. saccharin (SAC), cyclamate (CYC), acesulfame (ACE) and sucralose (SUC), in pig feed and manure from a pig farm and water samples from an on-farm wastewater treatment plant (WWTP) in Tianjin, China were measured and the mass loadings and removal efficiencies were assessed. Moreover, the levels of ASs in different layers of soil and vegetables in neighboring farmland that received manure fertilizers and wastewater from the farm were consecutively monitored for 60-80days. The SAC, CYC and ACE were widely determined in all kinds of the samples, while SUC was only found in few soil samples. The mass loadings of the ASs in pig feed were estimated up to 311kg/year for SAC, 59.1kg/year for CYC, and 17.1kg/year for ACE, respectively. The fractions of the total mass of ASs excreted via manure were estimated to be 36.0% for SAC, 59.4% for CYC, and 36.7% for ACE as compared to those in pig feed. High removal efficiencies (>90%) of ASs in the on-farm WWTP was achieved. In greenhouse soils, CYC, SAC, ACE, and SUC were degraded quickly, with half-lives of 4.3-5.9 d, 2.7-4.2 d, 8.4-12.3 d, and 7.3-10.8 d, respectively. Lower levels of ASs were found in deeper soil layer (20-30cm). The ASs were considerably absorbed by plants when the ASs' concentrations were high in soil. This study presents the first comprehensive overview of ASs fate from a pig farm to the neighboring agricultural ecosystem. Copyright © 2017. Published by Elsevier B.V.

  16. Parasitism by Cuscuta pentagona Attenuates Host Plant Defenses against Insect Herbivores1

    PubMed Central

    Runyon, Justin B.; Mescher, Mark C.; De Moraes, Consuelo M.

    2008-01-01

    Considerable research has examined plant responses to concurrent attack by herbivores and pathogens, but the effects of attack by parasitic plants, another important class of plant-feeding organisms, on plant defenses against other enemies has not been explored. We investigated how attack by the parasitic plant Cuscuta pentagona impacted tomato (Solanum lycopersicum) defenses against the chewing insect beet armyworm (Spodoptera exigua; BAW). In response to insect feeding, C. pentagona-infested (parasitized) tomato plants produced only one-third of the antiherbivore phytohormone jasmonic acid (JA) produced by unparasitized plants. Similarly, parasitized tomato, in contrast to unparasitized plants, failed to emit herbivore-induced volatiles after 3 d of BAW feeding. Although parasitism impaired antiherbivore defenses, BAW growth was slower on parasitized tomato leaves. Vines of C. pentagona did not translocate JA from BAW-infested plants: amounts of JA in parasite vines grown on caterpillar-fed and control plants were similar. Parasitized plants generally contained more salicylic acid (SA), which can inhibit JA in some systems. Parasitized mutant (NahG) tomato plants deficient in SA produced more JA in response to insect feeding than parasitized wild-type plants, further suggesting cross talk between the SA and JA defense signaling pathways. However, JA induction by BAW was still reduced in parasitized compared to unparasitized NahG, implying that other factors must be involved. We found that parasitized plants were capable of producing induced volatiles when experimentally treated with JA, indicating that resource depletion by the parasite does not fully explain the observed attenuation of volatile response to herbivore feeding. Collectively, these findings show that parasitic plants can have important consequences for host plant defense against herbivores. PMID:18165323

  17. Host plant species determines symbiotic bacterial community mediating suppression of plant defenses.

    PubMed

    Chung, Seung Ho; Scully, Erin D; Peiffer, Michelle; Geib, Scott M; Rosa, Cristina; Hoover, Kelli; Felton, Gary W

    2017-01-03

    Herbivore associated bacteria are vital mediators of plant and insect interactions. Host plants play an important role in shaping the gut bacterial community of insects. Colorado potato beetles (CPB; Leptinotarsa decemlineata) use several Solanum plants as hosts in their natural environment. We previously showed that symbiotic gut bacteria from CPB larvae suppressed jasmonate (JA)-induced defenses in tomato. However, little is known about how changes in the bacterial community may be involved in the manipulation of induced defenses in wild and cultivated Solanum plants of CPB. Here, we examined suppression of JA-mediated defense in wild and cultivated hosts of CPB by chemical elicitors and their symbiotic bacteria. Furthermore, we investigated associations between the gut bacterial community and suppression of plant defenses using 16 S rRNA amplicon sequencing. Symbiotic bacteria decreased plant defenses in all Solanum hosts and there were different gut bacterial communities in CPB fed on different host plants. When larvae were reared on different hosts, defense suppression differed among host plants. These results demonstrate that host plants influence herbivore gut bacterial communities and consequently affect the herbivore's ability to manipulate JA-mediated plant defenses. Thus, the presence of symbiotic bacteria that suppress plant defenses might help CPB adapt to host plants.

  18. Host plant species determines symbiotic bacterial community mediating suppression of plant defenses

    PubMed Central

    Chung, Seung Ho; Scully, Erin D.; Peiffer, Michelle; Geib, Scott M.; Rosa, Cristina; Hoover, Kelli; Felton, Gary W.

    2017-01-01

    Herbivore associated bacteria are vital mediators of plant and insect interactions. Host plants play an important role in shaping the gut bacterial community of insects. Colorado potato beetles (CPB; Leptinotarsa decemlineata) use several Solanum plants as hosts in their natural environment. We previously showed that symbiotic gut bacteria from CPB larvae suppressed jasmonate (JA)-induced defenses in tomato. However, little is known about how changes in the bacterial community may be involved in the manipulation of induced defenses in wild and cultivated Solanum plants of CPB. Here, we examined suppression of JA-mediated defense in wild and cultivated hosts of CPB by chemical elicitors and their symbiotic bacteria. Furthermore, we investigated associations between the gut bacterial community and suppression of plant defenses using 16 S rRNA amplicon sequencing. Symbiotic bacteria decreased plant defenses in all Solanum hosts and there were different gut bacterial communities in CPB fed on different host plants. When larvae were reared on different hosts, defense suppression differed among host plants. These results demonstrate that host plants influence herbivore gut bacterial communities and consequently affect the herbivore’s ability to manipulate JA-mediated plant defenses. Thus, the presence of symbiotic bacteria that suppress plant defenses might help CPB adapt to host plants. PMID:28045052

  19. Teaching Science Down on the Farm

    ERIC Educational Resources Information Center

    Hicks, Debbie

    2016-01-01

    Throughout the United Kingdom's (UK's) primary science curriculum, there are numerous opportunities for teachers to use the farming industry as a rich and engaging real-world context for science learning. Teachers can focus on the animals and plants on the farm as subjects for children to learn about life processes. They can turn attention…

  20. 50 CFR 14.23 - Live farm-raised fish and farm-raised fish eggs.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... OF WILDLIFE AND PLANTS IMPORTATION, EXPORTATION, AND TRANSPORTATION OF WILDLIFE Importation and... and farm-raised fish eggs meet the definition of “bred in captivity” as stated in 50 CFR 17.3. Except...

  1. Organic Farming Improves Pollination Success in Strawberries

    PubMed Central

    Andersson, Georg K. S.; Rundlöf, Maj; Smith, Henrik G.

    2012-01-01

    Pollination of insect pollinated crops has been found to be correlated to pollinator abundance and diversity. Since organic farming has the potential to mitigate negative effects of agricultural intensification on biodiversity, it may also benefit crop pollination, but direct evidence of this is scant. We evaluated the effect of organic farming on pollination of strawberry plants focusing on (1) if pollination success was higher on organic farms compared to conventional farms, and (2) if there was a time lag from conversion to organic farming until an effect was manifested. We found that pollination success and the proportion of fully pollinated berries were higher on organic compared to conventional farms and this difference was already evident 2–4 years after conversion to organic farming. Our results suggest that conversion to organic farming may rapidly increase pollination success and hence benefit the ecosystem service of crop pollination regarding both yield quantity and quality. PMID:22355380

  2. Integrating gene flow, crop biology, and farm management in on-farm conservation of avocado (Persea americana, Lauraceae).

    PubMed

    Birnbaum, Kenneth; Desalle, Rob; Peters, Charles M; Benfey, Philip N

    2003-11-01

    Maintaining crop diversity on farms where cultivars can evolve is a conservation goal, but few tools are available to assess the long-term maintenance of genetic diversity on farms. One important issue for on-farm conservation is gene flow from crops with a narrow genetic base into related populations that are genetically diverse. In a case study of avocado (Persea americana var. americana) in one of its centers of diversity (San Jerónimo, Costa Rica), we used 10 DNA microsatellite markers in a parentage analysis to estimate gene flow from commercialized varieties into a traditional crop population. Five commercialized genotypes comprised nearly 40% of orchard trees, but they contributed only about 14.5% of the gametes to the youngest cohort of trees. Although commercialized varieties and the diverse population were often planted on the same farm, planting patterns appeared to keep the two types of trees separated on small scales, possibly explaining the limited gene flow. In a simulation that combined gene flow estimates, crop biology, and graft tree management, loss of allelic diversity was less than 10% over 150 yr, and selection was effective in retaining desirable alleles in the diverse subpopulation. Simulations also showed that, in addition to gene flow, managing the genetic makeup and life history traits of the invasive commercialized varieties could have a significant impact on genetic diversity in the target population. The results support the feasibility of on-farm crop conservation, but simulations also showed that higher levels of gene flow could lead to severe losses of genetic diversity even if farmers continue to plant diverse varieties.

  3. The role of renewable energy on animal farms

    NASA Astrophysics Data System (ADS)

    Csatári, Nándor; Vántus, András

    2015-04-01

    The recent measures in the European Union promote the usage of renewable energies and enhancing the energy efficiency. These measures also effect agriculture, on the one hand by using biofuels mixed into fuel for machinery. Besides biofuels animal farms have opportunities in using renewable energy in several other ways. There are sectors in animal farming, where the energy demand is continuously high in electricity (e.g. forage grinders, mixers, milk coolers, air ventilation systems) or in heating (e.g. stables for poultry or piglets). Beside the energy demand in agricultural sector there are several products and side products suitable for energy production. For example different kinds of organic manures and corn silage could be raw materials for biogas production; plant residues like cereal straw and corn stalk bales could be combusted in boilers. Furthermore solar cells or solar collectors can be mounted on the big roof surfaces of animal farm buildings. Among animal farming sectors, dairy farming in the most energy intensive, and uses the widest variety of energy forms. It is often mentioned as the "heavy industry" of animal farming. In this research 14 dairy farms were examined in Hajdú-Bihar County in the topic of energy demand, renewable energy usage. The questioned farms covers 35% of the dairy cow population in Hajdú-Bihar County. The questions covered the general attributes of the farms and the details of the (existing or planned) renewable energy application. In terms of economic analysis saving, the investment return time and the employment effect was examined. The results show wide variety of applied renewable energy application. Fifty percent of farms uses at least one kind of renewable energy. Two biogas plants, 6 boilers for solid biomass, 2 solar cells. Regarding employment effect biogas plants created some full time workplaces, biomass boilers also needs some work hours to maintain, but none of the farms applied more labour. Besides renewable

  4. Induced plant-defenses suppress herbivore reproduction but also constrain predation of their offspring.

    PubMed

    Ataide, Livia M S; Pappas, Maria L; Schimmel, Bernardus C J; Lopez-Orenes, Antonio; Alba, Juan M; Duarte, Marcus V A; Pallini, Angelo; Schuurink, Robert C; Kant, Merijn R

    2016-11-01

    Inducible anti-herbivore defenses in plants are predominantly regulated by jasmonic acid (JA). On tomato plants, most genotypes of the herbivorous generalist spider mite Tetranychus urticae induce JA defenses and perform poorly on it, whereas the Solanaceae specialist Tetranychus evansi, who suppresses JA defenses, performs well on it. We asked to which extent these spider mites and the predatory mite Phytoseiulus longipes preying on these spider mites eggs are affected by induced JA-defenses. By artificially inducing the JA-response of the tomato JA-biosynthesis mutant def-1 using exogenous JA and isoleucine (Ile), we first established the relationship between endogenous JA-Ile-levels and the reproductive performance of spider mites. For both mite species we observed that they produced more eggs when levels of JA-Ile were low. Subsequently, we allowed predatory mites to prey on spider mite-eggs derived from wild-type tomato plants, def-1 and JA-Ile-treated def-1 and observed that they preferred, and consumed more, eggs produced on tomato plants with weak JA defenses. However, predatory mite oviposition was similar across treatments. Our results show that induced JA-responses negatively affect spider mite performance, but positively affect the survival of their offspring by constraining egg-predation. Copyright © 2016 The Authors. Published by Elsevier Ireland Ltd.. All rights reserved.

  5. Extracellular ATP Acts on Jasmonate Signaling to Reinforce Plant Defense.

    PubMed

    Tripathi, Diwaker; Zhang, Tong; Koo, Abraham J; Stacey, Gary; Tanaka, Kiwamu

    2018-01-01

    Damaged cells send various signals to stimulate defense responses. Recent identification and genetic studies of the plant purinoceptor, P2K1 (also known as DORN1), have demonstrated that extracellular ATP is a signal involved in plant stress responses, including wounding, perhaps to evoke plant defense. However, it remains largely unknown how extracellular ATP induces plant defense responses. Here, we demonstrate that extracellular ATP induces plant defense mediated through activation of the intracellular signaling of jasmonate (JA), a well-characterized defense hormone. In Arabidopsis ( Arabidopsis thaliana ) leaves, ATP pretreatment induced resistance against the necrotrophic fungus, Botrytis cinerea The induced resistance was enhanced in the P2K1 receptor overexpression line, but reduced in the receptor mutant, dorn1 - 3 Mining the transcriptome data revealed that ATP induces a set of JA-induced genes. In addition, the P2K1-associated coexpression network contains defense-related genes, including those encoding jasmonate ZIM-domain (JAZ) proteins, which play key roles as repressors of JA signaling. We examined whether extracellular ATP impacts the stability of JAZ1 in Arabidopsis. The results showed that the JAZ1 stability decreased in response to ATP addition in a proteasome-dependent manner. This reduction required intracellular signaling via second messengers-cytosolic calcium, reactive oxygen species, and nitric oxide. Interestingly, the ATP-induced JAZ1 degradation was attenuated in the JA receptor mutant, coi1 , but not in the JA biosynthesis mutant, aos , or upon addition of JA biosynthesis inhibitors. Immunoprecipitation analysis demonstrated that ATP increases the interaction between COI1 and JAZ1, suggesting direct cross talk between extracellular ATP and JA in intracellular signaling events. Taken together, these results suggest that extracellular ATP signaling directly impacts the JA signaling pathway to maximize plant defense responses. © 2018

  6. Comparative Effectiveness of Potential Elicitors of Plant Resistance against Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) in Four Crop Plants.

    PubMed

    Gordy, John W; Leonard, B Rogers; Blouin, David; Davis, Jeffrey A; Stout, Michael J

    2015-01-01

    Feeding by insect herbivores activates plant signaling pathways, resulting in the enhanced production of secondary metabolites and other resistance-related traits by injured plants. These traits can reduce insect fitness, deter feeding, and attract beneficial insects. Organic and inorganic chemicals applied as a foliar spray, seed treatment, or soil drench can activate these plant responses. Azelaic acid (AA), benzothiadiazole (BTH), gibberellic acid (GA), harpin, and jasmonic acid (JA) are thought to directly mediate plant responses to pathogens and herbivores or to mimic compounds that do. The effects of these potential elicitors on the induction of plant defenses were determined by measuring the weight gains of fall armyworm, Spodoptera frugiperda (J. E. Smith) (FAW) (Lepidoptera: Noctuidae) larvae on four crop plants, cotton, corn, rice, and soybean, treated with the compounds under greenhouse conditions. Treatment with JA consistently reduced growth of FAW reared on treated cotton and soybean. In contrast, FAW fed BTH- and harpin-treated cotton and soybean tissue gained more weight than those fed control leaf tissue, consistent with negative crosstalk between the salicylic acid and JA signaling pathways. No induction or inconsistent induction of resistance was observed in corn and rice. Follow-up experiments showed that the co-application of adjuvants with JA failed to increase the effectiveness of induction by JA and that soybean looper [Chrysodeixis includens (Walker)], a relative specialist on legumes, was less affected by JA-induced responses in soybean than was the polyphagous FAW. Overall, the results of these experiments demonstrate that the effectiveness of elicitors as a management tactic will depend strongly on the identities of the crop, the pest, and the elicitor involved.

  7. Understanding sources of sea lice for salmon farms in Chile.

    PubMed

    Kristoffersen, A B; Rees, E E; Stryhn, H; Ibarra, R; Campisto, J-L; Revie, C W; St-Hilaire, S

    2013-08-01

    The decline of fisheries over recent decades and a growing human population has coincided with an increase in aquaculture production. As farmed fish densities increase, so have their rates of infectious diseases, as predicted by the theory of density-dependent disease transmission. One of the pathogen that has increased with the growth of salmon farming is sea lice. Effective management of this pathogen requires an understanding of the spatial scale of transmission. We used a two-part multi-scale model to account for the zero-inflated data observed in weekly sea lice abundance levels on rainbow trout and Atlantic salmon farms in Chile, and to assess internal (farm) and external (regional) sources of sea lice infection. We observed that the level of juvenile sea lice was higher on farms that were closer to processing plants with fish holding facilities. Further, evidence for sea lice exposure from the surrounding area was supported by a strong positive correlation between the level of juvenile sea lice on a farm and the number of gravid females on neighboring farms within 30 km two weeks prior. The relationship between external sources of sea lice from neighboring farms and juvenile sea lice on a farm was one of the strongest detected in our multivariable model. Our findings suggest that the management of sea lice should be coordinated between farms and should include all farms and processing plants with holding facilities within a relatively large geographic area. Understanding the contribution of pathogens on a farm from different sources is an important step in developing effective control strategies. Crown Copyright © 2013. Published by Elsevier B.V. All rights reserved.

  8. Lunar Farming: Achieving Maximum Yield for the Exploration of Space

    NASA Technical Reports Server (NTRS)

    Salisbury, Frank B.

    1991-01-01

    A look at what it might be like on a lunar farm in the year 2020 is provided from the point of view of the farmer. Of necessity, the farm would be a Controlled Ecological (or Environment) Life-Support System (CELSS) or a bioregenerative life-support system. Topics covered in the imaginary trip through the farm are the light, water, gasses, crops, the medium used for plantings, and the required engineering. The CELSS is designed with four functioning parts: (1) A plant-production facility with higher plants and algae; (2) food technology kitchens; (3) waste processing and recycling facilities; and (4) control systems. In many cases there is not yet enough information to be sure about matters discussed, but the exercise in imagination pinpoints a number of areas that still need considerable research to resolve the problems perceived.

  9. Plant hormone jasmonate prioritizes defense over growth by interfering with gibberellin signaling cascade.

    PubMed

    Yang, Dong-Lei; Yao, Jian; Mei, Chuan-Sheng; Tong, Xiao-Hong; Zeng, Long-Jun; Li, Qun; Xiao, Lang-Tao; Sun, Tai-ping; Li, Jigang; Deng, Xing-Wang; Lee, Chin Mei; Thomashow, Michael F; Yang, Yinong; He, Zuhua; He, Sheng Yang

    2012-05-08

    Plants must effectively defend against biotic and abiotic stresses to survive in nature. However, this defense is costly and is often accompanied by significant growth inhibition. How plants coordinate the fluctuating growth-defense dynamics is not well understood and remains a fundamental question. Jasmonate (JA) and gibberellic acid (GA) are important plant hormones that mediate defense and growth, respectively. Binding of bioactive JA or GA ligands to cognate receptors leads to proteasome-dependent degradation of specific transcriptional repressors (the JAZ or DELLA family of proteins), which, at the resting state, represses cognate transcription factors involved in defense (e.g., MYCs) or growth [e.g. phytochrome interacting factors (PIFs)]. In this study, we found that the coi1 JA receptor mutants of rice (a domesticated monocot crop) and Arabidopsis (a model dicot plant) both exhibit hallmark phenotypes of GA-hypersensitive mutants. JA delays GA-mediated DELLA protein degradation, and the della mutant is less sensitive to JA for growth inhibition. Overexpression of a selected group of JAZ repressors in Arabidopsis plants partially phenocopies GA-associated phenotypes of the coi1 mutant, and JAZ9 inhibits RGA (a DELLA protein) interaction with transcription factor PIF3. Importantly, the pif quadruple (pifq) mutant no longer responds to JA-induced growth inhibition, and overexpression of PIF3 could partially overcome JA-induced growth inhibition. Thus, a molecular cascade involving the COI1-JAZ-DELLA-PIF signaling module, by which angiosperm plants prioritize JA-mediated defense over growth, has been elucidated.

  10. Terraces and contour farming

    USDA-ARS?s Scientific Manuscript database

    Terraces are earthen embankments constructed across the prevailing field land slope. They have been used in differing forms for thousands of years in an attempt to protect steep land slopes from runoff induced erosion. Contour farming, where tillage and planting create ridges and furrows at nearly...

  11. Use of meteorological information in the risk analysis of a mixed wind farm and solar

    NASA Astrophysics Data System (ADS)

    Mengelkamp, H.-T.; Bendel, D.

    2010-09-01

    Use of meteorological information in the risk analysis of a mixed wind farm and solar power plant portfolio H.-T. Mengelkamp*,** , D. Bendel** *GKSS Research Center Geesthacht GmbH **anemos Gesellschaft für Umweltmeteorologie mbH The renewable energy industry has rapidly developed during the last two decades and so have the needs for high quality comprehensive meteorological services. It is, however, only recently that international financial institutions bundle wind farms and solar power plants and offer shares in these aggregate portfolios. The monetary value of a mixed wind farm and solar power plant portfolio is determined by legal and technical aspects, the expected annual energy production of each wind farm and solar power plant and the associated uncertainty of the energy yield estimation or the investment risk. Building an aggregate portfolio will reduce the overall uncertainty through diversification in contrast to the single wind farm/solar power plant energy yield uncertainty. This is similar to equity funds based on a variety of companies or products. Meteorological aspects contribute to the diversification in various ways. There is the uncertainty in the estimation of the expected long-term mean energy production of the wind and solar power plants. Different components of uncertainty have to be considered depending on whether the power plant is already in operation or in the planning phase. The uncertainty related to a wind farm in the planning phase comprises the methodology of the wind potential estimation and the uncertainty of the site specific wind turbine power curve as well as the uncertainty of the wind farm effect calculation. The uncertainty related to a solar power plant in the pre-operational phase comprises the uncertainty of the radiation data base and that of the performance curve. The long-term mean annual energy yield of operational wind farms and solar power plants is estimated on the basis of the actual energy production and it

  12. Plant defenses against parasitic plants show similarities to those induced by herbivores and pathogens

    PubMed Central

    Runyon, Justin B; Mescher, Mark C

    2010-01-01

    Herbivores and pathogens come quickly to mind when one thinks of the biotic challenges faced by plants. Important but less appreciated enemies are parasitic plants, which can have important consequences for the fitness and survival of their hosts. Our knowledge of plant perception, signaling and response to herbivores and pathogens has expanded rapidly in recent years, but information is generally lacking for parasitic species. In a recent paper we reported that some of the same defense responses induced by herbivores and pathogens—notably increases in jasmonic acid (JA), salicylic acid (SA), and a hypersensitive-like response (HLR)—also occur in tomato plants upon attack by the parasitic plant Cuscuta pentagona (field dodder). Parasitism induced a distinct pattern of JA and SA accumulation, and growth trials using genetically-altered tomato hosts suggested that both JA and SA govern effective defenses against the parasite, though the extent of the response varied with host plant age. Here we discuss similarities between the induced responses we observed in response to Cuscuta parasitism to those previously described for herbivores and pathogens and present new data showing that trichomes should be added to the list of plant defenses that act against multiple enemies and across kingdoms. PMID:20495380

  13. Plant defenses against parasitic plants show similarities to those induced by herbivores and pathogens.

    PubMed

    Runyon, Justin B; Mescher, Mark C; De Moraes, Consuelo M

    2010-08-01

    Herbivores and pathogens come quickly to mind when one thinks of the biotic challenges faced by plants. Important but less appreciated enemies are parasitic plants, which can have important consequences for the fitness and survival of their hosts. Our knowledge of plant perception, signaling, and response to herbivores and pathogens has expanded rapidly in recent years, but information is generally lacking for parasitic species. In a recent paper we reported that some of the same defense responses induced by herbivores and pathogens--notably increases in jasmonic acid (JA), salicylic acid (SA), and a hypersensitive-like response (HLR)--also occur in tomato plants upon attack by the parasitic plant Cuscuta pentagona (field dodder). Parasitism induced a distinct pattern of JA and SA accumulation, and growth trials using genetically-altered tomato hosts suggested that both JA and SA govern effective defenses against the parasite, though the extent of the response varied with host plant age. Here we discuss similarities between the induced responses we observed in response to Cuscuta parasitism to those previously described for herbivores and pathogens and present new data showing that trichomes should be added to the list of plant defenses that act against multiple enemies and across Kingdoms.

  14. Herbivore-induced blueberry volatiles and intra-plant signaling.

    PubMed

    Rodriguez-Saona, Cesar R

    2011-12-18

    Herbivore-induced plant volatiles (HIPVs) are commonly emitted from plants after herbivore attack. These HIPVs are mainly regulated by the defensive plant hormone jasmonic acid (JA) and its volatile derivative methyl jasmonate (MeJA). Over the past 3 decades researchers have documented that HIPVs can repel or attract herbivores, attract the natural enemies of herbivores, and in some cases they can induce or prime plant defenses prior to herbivore attack. In a recent paper, I reported that feeding by gypsy moth caterpillars, exogenous MeJA application, and mechanical damage induce the emissions of volatiles from blueberry plants, albeit differently. In addition, blueberry branches respond to HIPVs emitted from neighboring branches of the same plant by increasing the levels of JA and resistance to herbivores (i.e., direct plant defenses), and by priming volatile emissions (i.e., indirect plant defenses). Similar findings have been reported recently for sagebrush, poplar, and lima beans. Here, I describe a push-pull method for collecting blueberry volatiles induced by herbivore (gypsy moth) feeding, exogenous MeJA application, and mechanical damage. The volatile collection unit consists of a 4 L volatile collection chamber, a 2-piece guillotine, an air delivery system that purifies incoming air, and a vacuum system connected to a trap filled with Super-Q adsorbent to collect volatiles. Volatiles collected in Super-Q traps are eluted with dichloromethane and then separated and quantified using Gas Chromatography (GC). This volatile collection method was used in my study to investigate the volatile response of undamaged branches to exposure to volatiles from herbivore-damaged branches within blueberry plants. These methods are described here. Briefly, undamaged blueberry branches are exposed to HIPVs from neighboring branches within the same plant. Using the same techniques described above, volatiles emitted from branches after exposure to HIPVs are collected and

  15. Prokaryotic Diversity in the Rhizosphere of Organic, Intensive, and Transitional Coffee Farms in Brazil.

    PubMed

    Caldwell, Adam Collins; Silva, Lívia Carneiro Fidéles; da Silva, Cynthia Canêdo; Ouverney, Cleber Costa

    2015-01-01

    Despite a continuous rise in consumption of coffee over the past 60 years and recent studies showing positive benefits linked to human health, intensive coffee farming practices have been associated with environmental damage, risks to human health, and reductions in biodiversity. In contrast, organic farming has become an increasingly popular alternative, with both environmental and health benefits. This study aimed to characterize and determine the differences in the prokaryotic soil microbiology of three Brazilian coffee farms: one practicing intensive farming, one practicing organic farming, and one undergoing a transition from intensive to organic practices. Soil samples were collected from 20 coffee plant rhizospheres (soil directly influenced by the plant root exudates) and 10 control sites (soil 5 m away from the coffee plantation) at each of the three farms for a total of 90 samples. Profiling of 16S rRNA gene V4 regions revealed high levels of prokaryotic diversity in all three farms, with thousands of species level operational taxonomic units identified in each farm. Additionally, a statistically significant difference was found between each farm's coffee rhizosphere microbiome, as well as between coffee rhizosphere soils and control soils. Two groups of prokaryotes associated with the nitrogen cycle, the archaeal genus Candidatus Nitrososphaera and the bacterial order Rhizobiales were found to be abundant and statistically different in composition between the three farms and in inverse relationship to each other. Many of the nitrogen-fixing genera known to enhance plant growth were found in low numbers (e.g. Rhizobium, Agrobacter, Acetobacter, Rhodospirillum, Azospirillum), but the families in which they belong had some of the highest relative abundance in the dataset, suggesting many new groups may exist in these samples that can be further studied as potential plant growth-promoting bacteria to improve coffee production while diminishing negative

  16. Design Improvements in Underground Watering System for Small Local Farming Industries

    NASA Astrophysics Data System (ADS)

    Ariff, T. F.; Tukiman, N. H. A.; Bahar, R.

    2016-02-01

    The implementation of underground watering system is basically to supply crops with enough quantities of water. In Malaysia, most farming industries use sprinkler irrigation system. The water is only distributed over the surface whilst the roots actually need water the most. Thus, this research is conducted to design the improvements of watering system for small local farming industries by using underground watering system. Design improvements of the watering system had been done using CATIA software. The design had been fabricated using rapid prototyping/3D printer, tested and evaluated by conducting experiments. Four different plants were prepared and labelled as Plant A, Plant B, Plant C, and Plant D. Plant A and Plant C were not be equipped with the underground watering device while Plant B and Plant D were equipped with the device. The growth of every plant is measured in terms of height, number of newly grown leaves, number of flowers and number of fruits for the duration of 60 days. The plant equipped with the device has the quickest growth measurement (59.68%), continued to produce new leaves rapidly (89.20%), and produced the most number of flowers (19 flowers) and fruits (15 fruits) when compared with the plants without the underground watering device. The difference in growth development is very significant. Therefore, the underground watering system does have a positive impact in nourishing the plant from the root efficiently and can be used productively in small local farming industries.

  17. A global synthesis of the effects of diversified farming systems on arthropod diversity within fields and across agricultural landscapes.

    PubMed

    Lichtenberg, Elinor M; Kennedy, Christina M; Kremen, Claire; Batáry, Péter; Berendse, Frank; Bommarco, Riccardo; Bosque-Pérez, Nilsa A; Carvalheiro, Luísa G; Snyder, William E; Williams, Neal M; Winfree, Rachael; Klatt, Björn K; Åström, Sandra; Benjamin, Faye; Brittain, Claire; Chaplin-Kramer, Rebecca; Clough, Yann; Danforth, Bryan; Diekötter, Tim; Eigenbrode, Sanford D; Ekroos, Johan; Elle, Elizabeth; Freitas, Breno M; Fukuda, Yuki; Gaines-Day, Hannah R; Grab, Heather; Gratton, Claudio; Holzschuh, Andrea; Isaacs, Rufus; Isaia, Marco; Jha, Shalene; Jonason, Dennis; Jones, Vincent P; Klein, Alexandra-Maria; Krauss, Jochen; Letourneau, Deborah K; Macfadyen, Sarina; Mallinger, Rachel E; Martin, Emily A; Martinez, Eliana; Memmott, Jane; Morandin, Lora; Neame, Lisa; Otieno, Mark; Park, Mia G; Pfiffner, Lukas; Pocock, Michael J O; Ponce, Carlos; Potts, Simon G; Poveda, Katja; Ramos, Mariangie; Rosenheim, Jay A; Rundlöf, Maj; Sardiñas, Hillary; Saunders, Manu E; Schon, Nicole L; Sciligo, Amber R; Sidhu, C Sheena; Steffan-Dewenter, Ingolf; Tscharntke, Teja; Veselý, Milan; Weisser, Wolfgang W; Wilson, Julianna K; Crowder, David W

    2017-11-01

    Agricultural intensification is a leading cause of global biodiversity loss, which can reduce the provisioning of ecosystem services in managed ecosystems. Organic farming and plant diversification are farm management schemes that may mitigate potential ecological harm by increasing species richness and boosting related ecosystem services to agroecosystems. What remains unclear is the extent to which farm management schemes affect biodiversity components other than species richness, and whether impacts differ across spatial scales and landscape contexts. Using a global metadataset, we quantified the effects of organic farming and plant diversification on abundance, local diversity (communities within fields), and regional diversity (communities across fields) of arthropod pollinators, predators, herbivores, and detritivores. Both organic farming and higher in-field plant diversity enhanced arthropod abundance, particularly for rare taxa. This resulted in increased richness but decreased evenness. While these responses were stronger at local relative to regional scales, richness and abundance increased at both scales, and richness on farms embedded in complex relative to simple landscapes. Overall, both organic farming and in-field plant diversification exerted the strongest effects on pollinators and predators, suggesting these management schemes can facilitate ecosystem service providers without augmenting herbivore (pest) populations. Our results suggest that organic farming and plant diversification promote diverse arthropod metacommunities that may provide temporal and spatial stability of ecosystem service provisioning. Conserving diverse plant and arthropod communities in farming systems therefore requires sustainable practices that operate both within fields and across landscapes. © 2017 John Wiley & Sons Ltd.

  18. Seed-based expression systems for plant molecular farming.

    PubMed

    Boothe, Joseph; Nykiforuk, Cory; Shen, Yin; Zaplachinski, Steven; Szarka, Steven; Kuhlman, Philip; Murray, Elizabeth; Morck, Douglas; Moloney, Maurice M

    2010-06-01

    The evolution of the seed system provides enormous adaptability to the gymnosperms and angiosperms, because of the properties of dormancy, nutrient storage and seedling vigour. Many of the unique properties of seeds can be exploited in molecular farming applications, particularly where it is desirable to produce large quantities of a recombinant protein. Seeds of transgenic plants have been widely used to generate a raw material for the extraction and isolation of proteins and polypeptides, which can be processed into valuable biopharmaceuticals. The factors that control high-level accumulation of recombinant proteins in seed are reviewed in the following paragraphs. These include promoters and enhancers, which regulate transcript abundance. However, it is shown that subcellular trafficking and targeting of the desired polypeptides or proteins play a crucial role in their accumulation at economically useful levels. Seeds have proven to be versatile hosts for recombinant proteins of all types, including peptides or short and long polypeptides as well as complex, noncontiguous proteins like antibodies and other immunoglobulins. The extraction and recovery of recombinant proteins from seeds is greatly assisted by their dormancy properties, because this allows for long-term stability of stored products including recombinant proteins and a decoupling of processing from the growth and harvest cycles. Furthermore, the low water content and relatively low bioload of seeds help greatly in designing cost-effective manufacturing processes for the desired active pharmaceutical ingredient. The development of cGMP processes based on seed-derived materials has only been attempted by a few groups to date, but we provide a review of the key issues and criteria based on interactions with Food and Drug Administration and European Medicines Agency. This article uses 'case studies' to highlight the utility of seeds as vehicles for pharmaceutical production including: insulin, human

  19. The Farm--Its Function and Future. Environmental Ecological Education Project. Revised.

    ERIC Educational Resources Information Center

    Parkway School District, Chesterfield, MO.

    This unit, designed for use in the primary grades (K-3), focuses on the farm and its functions. The various aspects of farming are discussed from an ecological and environmental point of view through such topics as soil, plants, animals, machinery, production of food, job opportunities, and the future of the farm. There is also a comparison of the…

  20. MYC2 Orchestrates a Hierarchical Transcriptional Cascade That Regulates Jasmonate-Mediated Plant Immunity in Tomato.

    PubMed

    Du, Minmin; Zhao, Jiuhai; Tzeng, David T W; Liu, Yuanyuan; Deng, Lei; Yang, Tianxia; Zhai, Qingzhe; Wu, Fangming; Huang, Zhuo; Zhou, Ming; Wang, Qiaomei; Chen, Qian; Zhong, Silin; Li, Chang-Bao; Li, Chuanyou

    2017-08-01

    The hormone jasmonate (JA), which functions in plant immunity, regulates resistance to pathogen infection and insect attack through triggering genome-wide transcriptional reprogramming in plants. We show that the basic helix-loop-helix transcription factor (TF) MYC2 in tomato ( Solanum lycopersicum ) acts downstream of the JA receptor to orchestrate JA-mediated activation of both the wounding and pathogen responses. Using chromatin immunoprecipitation sequencing (ChIP-seq) coupled with RNA sequencing (RNA-seq) assays, we identified 655 MYC2-targeted JA-responsive genes. These genes are highly enriched in Gene Ontology categories related to TFs and the early response to JA, indicating that MYC2 functions at a high hierarchical level to regulate JA-mediated gene transcription. We also identified a group of MYC2-targeted TFs (MTFs) that may directly regulate the JA-induced transcription of late defense genes. Our findings suggest that MYC2 and its downstream MTFs form a hierarchical transcriptional cascade during JA-mediated plant immunity that initiates and amplifies transcriptional output. As proof of concept, we showed that during plant resistance to the necrotrophic pathogen Botrytis cinerea , MYC2 and the MTF JA2-Like form a transcription module that preferentially regulates wounding-responsive genes, whereas MYC2 and the MTF ETHYLENE RESPONSE FACTOR.C3 form a transcription module that preferentially regulates pathogen-responsive genes. © 2017 American Society of Plant Biologists. All rights reserved.

  1. Jasmonate response decay and defense metabolite accumulation contributes to age-regulated dynamics of plant insect resistance

    PubMed Central

    Mao, Ying-Bo; Liu, Yao-Qian; Chen, Dian-Yang; Chen, Fang-Yan; Fang, Xin; Hong, Gao-Jie; Wang, Ling-Jian; Wang, Jia-Wei; Chen, Xiao-Ya

    2017-01-01

    Immunity deteriorates with age in animals but comparatively little is known about the temporal regulation of plant resistance to herbivores. The phytohormone jasmonate (JA) is a key regulator of plant insect defense. Here, we show that the JA response decays progressively in Arabidopsis. We show that this decay is regulated by the miR156-targeted SQUAMOSA PROMOTER BINDING PROTEIN-LIKE9 (SPL9) group of proteins, which can interact with JA ZIM-domain (JAZ) proteins, including JAZ3. As SPL9 levels gradually increase, JAZ3 accumulates and the JA response is attenuated. We provide evidence that this pathway contributes to insect resistance in young plants. Interestingly however, despite the decay in JA response, older plants are still comparatively more resistant to both the lepidopteran generalist Helicoverpa armigera and the specialist Plutella xylostella, along with increased accumulation of glucosinolates. We propose a model whereby constitutive accumulation of defense compounds plays a role in compensating for age-related JA-response attenuation during plant maturation. PMID:28067238

  2. Xylella fastidiosa, a new plant pathogen that threatens global farming: Ecology, molecular biology, search for remedies.

    PubMed

    Bucci, Enrico M

    2018-07-12

    Recently, the emergence of an important alien plant pathogen in Europe was evident when the Olive Quick Decline Syndrome (OQDS), a previously unknown disease causing rapid scorching and death of the trees, invested with particular virulence a substantial portion of the vast olive wood of Southern Italy (Salento, part of the Apulia region). Early evidence indicated a connection between the OQDS and the gram-negative bacterium Xylella fastidiosa. This bacterium can target several important crops, so that researchers from all over the world have investigated its association with host plants and vectors, the molecular biology of the infection mechanism, and the molecular reaction of the infected plants. Potentially resistant or tolerant cultivars and molecular targets which might be useful to control the infection have been identified. In vitro tests of compounds active against Xylella have also been performed. In this contribution, the literature and the available data will be reviewed to provide an up-to-date picture of the currently available knowledge on the role of Xylella in OQDS, and in diseases of other plants, with focus on the emerging threats to European farming. Copyright © 2018 Elsevier Inc. All rights reserved.

  3. Three year field test of a plant growth promoting rhizobacteria enhanced phytoremediation system at a land farm for treatment of hydrocarbon waste.

    PubMed

    Gurska, Jolanta; Wang, Wenxi; Gerhardt, Karen E; Khalid, Aaron M; Isherwood, David M; Huang, Xiao-Dong; Glick, Bernard R; Greenberg, Bruce M

    2009-06-15

    Phytoremediation of total petroleum hydrocarbons (TPH) has the potential to be a sustainable waste management technology if it can be proven to be effective in the field. Over the past decade, our laboratory has developed a system which utilizes plant growth promoting rhizobacteria (PGPR) enhanced phytoremediation (PEP) that, following extensive greenhouse testing, was shown to be effective at remediating TPH from soils. This system consists of physical soil manipulation and plant growth following seed inoculation with PGPR. PGPR elicit biomass increases, particularly in roots, by minimizing plant stress in highly contaminated soils. Extensive development of the root system enhances degradation of contaminants by the plants and supports an active rhizosphere that effectively promotes TPH degradation by a broad microbial consortium. Following promising greenhouse trials, field tests of PEP were performed over a period of three years at a Southern Ontario site (approximately 130 g kg(-1) TPH) used for land farming of refinery hydrocarbon waste for many years. The low molecular weight fractions (the Canadian Council of Ministers of the Environment (CCME) fractions 1 and 2) were removed through land farming and bioremediation; the high molecular weight, recalcitrant fractions (CCME fractions 3 and 4) remained at high levels in the soil. Using PEP, we substantially remediated fractions 3 and 4, and lowered TPH from 130 g kg(-1) to approximately 50 g kg(-1) over a three year period. The amount of plant growth and extent of oil remediation were consistently enhanced by PGPR.

  4. Jasmonate Hormone: Regulating Synthesis of Reduced Carbon Compounds in Plants

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Browse, John

    Our original interest in understanding the role of jasmonate (JA) in regulating the final stages of stamen and pollen development led to our discovery of the JAZ repressors, and the molecular mechanism of JA action is now a second important focus of our research. The specific goals for this grant period are to: 1. Investigate the generation and clearance of the hormone with emphasis on the regulation of the OPR3 enzyme and the hydrolysis of JA-Ile. 2. Use dominant-negative and overexpression constructs to explore the role of the MYC5 transcription factor in initiating and regulating JA responses. 3. Investigate specificmore » JAZ protein interactions that will help us to recognize and understand the extended network of processes, such as sulfur nutrition, that interface with JA signaling. The COI1 F-Box protein is a JA-Ile coreceptor and coi1 mutant plants lack JA responses. We have tested the possibility that sites of JA action can be probed by using tissue-specific promoters to drive expression of a COI1-YFP fusion protein in coi1 mutant plants deficient in stamen and pollen function. When we expressed COI1 behind a filament-specific promoter (from the DAD1 gene), filament elongation was restored but not anther dehiscence or pollen function. Three tapetum specific promoters, all failed to restore any of these three functions but, unexpectedly, a promoter active in the stomium and epidermal cells, restored both pollen function and anther dehiscence. Most importantly, our results demonstrate the power of promoter::COI1-YFP constructs in revealing the primary sites of JA-regulated gene expression that control developmental and other responses in neighboring tissues. We now plan to use this new tool to test current hypotheses about JA action in other organs of the plant. The MYC2, MYC3, and MYC4 proteins are the primary transcription factors initiating defense and root growth responses to JA signaling. However, transgenic plants overexpressing these proteins do not

  5. Jasmonic acid distribution and action in plants: regulation during development and response to biotic and abiotic stress.

    PubMed Central

    Creelman, R A; Mullet, J E

    1995-01-01

    Jasmonic acid (JA) is a naturally occurring growth regulator found in higher plants. Several physiological roles have been described for this compound (or a related compound, methyl jasmonate) during plant development and in response to biotic and abiotic stress. To accurately determine JA levels in plant tissue, we have synthesized JA containing 13C for use as an internal standard with an isotopic composition of [225]:[224] 0.98:0.02 compared with [225]:[224] 0.15:0.85 for natural material. GC analysis (flame ionization detection and MS) indicate that the internal standard is composed of 92% 2-(+/-)-[13C]JA and 8% 2-(+/-)-7-iso-[13C]JA. In soybean plants, JA levels were highest in young leaves, flowers, and fruit (highest in the pericarp). In soybean seeds and seedlings, JA levels were highest in the youngest organs including the hypocotyl hook, plumule, and 12-h axis. In soybean leaves that had been dehydrated to cause a 15% decrease in fresh weight, JA levels increased approximately 5-fold within 2 h and declined to approximately control levels by 4 h. In contrast, a lag time of 1-2 h occurred before abscisic acid accumulation reached a maximum. These results will be discussed in the context of multiple pathways for JA biosynthesis and the role of JA in plant development and responses to environmental signals. PMID:11607536

  6. Jasmonic acid distribution and action in plants: regulation during development and response to biotic and abiotic stress.

    PubMed

    Creelman, R A; Mullet, J E

    1995-05-09

    Jasmonic acid (JA) is a naturally occurring growth regulator found in higher plants. Several physiological roles have been described for this compound (or a related compound, methyl jasmonate) during plant development and in response to biotic and abiotic stress. To accurately determine JA levels in plant tissue, we have synthesized JA containing 13C for use as an internal standard with an isotopic composition of [225]:[224] 0.98:0.02 compared with [225]:[224] 0.15:0.85 for natural material. GC analysis (flame ionization detection and MS) indicate that the internal standard is composed of 92% 2-(+/-)-[13C]JA and 8% 2-(+/-)-7-iso-[13C]JA. In soybean plants, JA levels were highest in young leaves, flowers, and fruit (highest in the pericarp). In soybean seeds and seedlings, JA levels were highest in the youngest organs including the hypocotyl hook, plumule, and 12-h axis. In soybean leaves that had been dehydrated to cause a 15% decrease in fresh weight, JA levels increased approximately 5-fold within 2 h and declined to approximately control levels by 4 h. In contrast, a lag time of 1-2 h occurred before abscisic acid accumulation reached a maximum. These results will be discussed in the context of multiple pathways for JA biosynthesis and the role of JA in plant development and responses to environmental signals.

  7. Research on large-scale wind farm modeling

    NASA Astrophysics Data System (ADS)

    Ma, Longfei; Zhang, Baoqun; Gong, Cheng; Jiao, Ran; Shi, Rui; Chi, Zhongjun; Ding, Yifeng

    2017-01-01

    Due to intermittent and adulatory properties of wind energy, when large-scale wind farm connected to the grid, it will have much impact on the power system, which is different from traditional power plants. Therefore it is necessary to establish an effective wind farm model to simulate and analyze the influence wind farms have on the grid as well as the transient characteristics of the wind turbines when the grid is at fault. However we must first establish an effective WTGs model. As the doubly-fed VSCF wind turbine has become the mainstream wind turbine model currently, this article first investigates the research progress of doubly-fed VSCF wind turbine, and then describes the detailed building process of the model. After that investigating the common wind farm modeling methods and pointing out the problems encountered. As WAMS is widely used in the power system, which makes online parameter identification of the wind farm model based on off-output characteristics of wind farm be possible, with a focus on interpretation of the new idea of identification-based modeling of large wind farms, which can be realized by two concrete methods.

  8. Spread of multidrug-resistant Escherichia coli harboring integron via swine farm waste water treatment plant.

    PubMed

    Park, Jin-Hyeong; Kim, Young-Ji; Binn-Kim; Seo, Kun-Ho

    2018-03-01

    Wastewater treatment plants (WWTPs) that release treated wastewater into the environment have emerged as a major threat to public health. In this study, we investigated Escherichia coli load and antibiotic-resistance profiles across different treatment processes at a swine farm WWTP. The frequency of the detection of class 1 and 2 integrons, and their association with antibiotic resistance, were also analyzed. Samples were obtained at each of five sampling sites that represented each processing step within the WWTP. The largest decrease in E. coli load was observed during the anaerobic digestion step (from 4.86 to 2.89log CFU/mL). Isolates resistant to β-lactam antibiotics were efficiently removed after a series of treatment steps, whereas the proportions of isolates resistant to non-β-lactam antibiotics and multidrug-resistant strains were maintained across treatments. The occurrence of integron-positive strains was not significantly different at the various sampling sites (43.4-70%; p>0.05). Of the class 1 integron-positive isolates, 17.9% harbored the integron-associated gene cassettes aadA2, aadA12, aadA22, and dfrA15. To the best of our knowledge, this is the first description of a class 1 integron containing the aadA12 gene cassette from a swine farm and the presence of a class 1 integron containing dfrA15 in E. coli. This suggests that novel antibiotic-resistance gene cassette arrays could be generated in swine farm WWTPs. Moreover, 75% of integron-positive strains were categorized as multidrug resistant, whereas only 15.4% of integron-negative strains were multidrug resistant (p<0.05), indicating that integrons may be responsible for mediating resistance in WWTPs. With regard to the occurrence of multidrug-resistant, integron-positive E. coli recovered from the final effluent, our results highlighted the potential risks associated with wastewater discharge from swine farm WWTPs in terms of the spread of antibiotic-resistant bacteria to the aquatic

  9. Prokaryotic Diversity in the Rhizosphere of Organic, Intensive, and Transitional Coffee Farms in Brazil

    PubMed Central

    Caldwell, Adam Collins; Silva, Lívia Carneiro Fidéles; da Silva, Cynthia Canêdo; Ouverney, Cleber Costa

    2015-01-01

    Despite a continuous rise in consumption of coffee over the past 60 years and recent studies showing positive benefits linked to human health, intensive coffee farming practices have been associated with environmental damage, risks to human health, and reductions in biodiversity. In contrast, organic farming has become an increasingly popular alternative, with both environmental and health benefits. This study aimed to characterize and determine the differences in the prokaryotic soil microbiology of three Brazilian coffee farms: one practicing intensive farming, one practicing organic farming, and one undergoing a transition from intensive to organic practices. Soil samples were collected from 20 coffee plant rhizospheres (soil directly influenced by the plant root exudates) and 10 control sites (soil 5 m away from the coffee plantation) at each of the three farms for a total of 90 samples. Profiling of 16S rRNA gene V4 regions revealed high levels of prokaryotic diversity in all three farms, with thousands of species level operational taxonomic units identified in each farm. Additionally, a statistically significant difference was found between each farm’s coffee rhizosphere microbiome, as well as between coffee rhizosphere soils and control soils. Two groups of prokaryotes associated with the nitrogen cycle, the archaeal genus Candidatus Nitrososphaera and the bacterial order Rhizobiales were found to be abundant and statistically different in composition between the three farms and in inverse relationship to each other. Many of the nitrogen-fixing genera known to enhance plant growth were found in low numbers (e.g. Rhizobium, Agrobacter, Acetobacter, Rhodospirillum, Azospirillum), but the families in which they belong had some of the highest relative abundance in the dataset, suggesting many new groups may exist in these samples that can be further studied as potential plant growth-promoting bacteria to improve coffee production while diminishing negative

  10. Jasmonate signaling in plant stress responses and development - active and inactive compounds.

    PubMed

    Wasternack, Claus; Strnad, Miroslav

    2016-09-25

    Jasmonates (JAs) are lipid-derived signals mediating plant responses to biotic and abiotic stresses and in plant development. Following the elucidation of each step in their biosynthesis and the important components of perception and signaling, several activators, repressors and co-repressors have been identified which contribute to fine-tuning the regulation of JA-induced gene expression. Many of the metabolic reactions in which JA participates, such as conjugation with amino acids, glucosylation, hydroxylation, carboxylation, sulfation and methylation, lead to numerous compounds with different biological activities. These metabolites may be highly active, partially active in specific processes or inactive. Hydroxylation, carboxylation and sulfation inactivate JA signaling. The precursor of JA biosynthesis, 12-oxo-phytodienoic acid (OPDA), has been identified as a JA-independent signaling compound. An increasing number of OPDA-specific processes is being identified. To conclude, the numerous JA compounds and their different modes of action allow plants to respond specifically and flexibly to alterations in the environment. Copyright © 2015 Elsevier B.V. All rights reserved.

  11. Opportunities of energy supply of farm holdings on the basis of small-scale renewable energy sources

    NASA Astrophysics Data System (ADS)

    Efendiev, A. M.; Nikolaev, Yu. E.; Evstaf'ev, D. P.

    2016-02-01

    One of the major national economic problems of Russia is raising of agricultural production, which will provide strategic security and sustainable supply of the population with provisions. Creation of subsidiary small holdings, farm holdings, and peasant farm holdings will require addressing issues of energy supply. At considerable distance of small farms from centralized energy systems (by fuel, electricity and thermal energy) it is proposed to create a system of local energy networks on the basis of low-powered power plants using renewable energy sources (RES). There is economic unreasonableness of use of imported components of small power plants. Creation of new combined small power plants on renewable energy sources produced by domestic manufacturers is recommended. Schemes of arrangements of small power plants based on renewable energy sources are proposed, variants and characteristics of a basic source are provided—biogas plants developed by the authors. Calculations revealed that heat and power supply of self-contained farms distant from small power plants based on renewable energy sources is 2.5-2.6 times cheaper than from centralized networks. Production of biogas through anaerobic fermentation of organic waste of cattle complexes is considered as the basis. The analysis of biowaste output in various cattle farms is carried out, and the volume of biogas is determined to meet the requirements of these farms in electrical and thermal energy. The objective of the present article is to study the possibility of creating small combined power plants in Russia based on renewable sources of energy for independent consumers.

  12. Intraspecific variation in a generalist herbivore accounts for differential induction and impact of host plant defences

    PubMed Central

    Kant, Merijn R; Sabelis, Maurice W; Haring, Michel A; Schuurink, Robert C

    2007-01-01

    Plants and herbivores are thought to be engaged in a coevolutionary arms race: rising frequencies of plants with anti-herbivore defences exert pressure on herbivores to resist or circumvent these defences and vice versa. Owing to its frequency-dependent character, the arms race hypothesis predicts that herbivores exhibit genetic variation for traits that determine how they deal with the defences of a given host plant phenotype. Here, we show the existence of distinct variation within a single herbivore species, the spider mite Tetranychus urticae, in traits that lead to resistance or susceptibility to jasmonate (JA)-dependent defences of a host plant but also in traits responsible for induction or repression of JA defences. We characterized three distinct lines of T. urticae that differentially induced JA-related defence genes and metabolites while feeding on tomato plants (Solanum lycopersicum). These lines were also differently affected by induced JA defences. The first line, which induced JA-dependent tomato defences, was susceptible to those defences; the second line also induced JA defences but was resistant to them; and the third, although susceptible to JA defences, repressed induction. We hypothesize that such intraspecific variation is common among herbivores living in environments with a diversity of plants that impose diverse selection pressure. PMID:18055390

  13. Urban farming activity towards sustainable wellbeing of urban dwellers

    NASA Astrophysics Data System (ADS)

    Othman, N.; Mohamad, M.; Latip, R. A.; Ariffin, M. H.

    2018-02-01

    In Malaysia, urban farming is viewed as a catalyst towards achieving the well-being of urban dwellers and natural environment. Urban farming is a strategy for Malaysia’s food and economic security, and as one of the foci in the agriculture transformation whereby urban dwellers are encouraged to participate in this activity. Previous study proved that urban farming can help to address social problems of food security, urban poverty and high living cost, also provides leisure and recreation among urban dwellers. Thus, this study investigates the best urban farming practices suitable for urban setting, environment and culture of urban dwellers. Data collection was done via questionnaire survey to urban farmers of a selected community garden in Subang Jaya, Selangor. Meanwhile, on-site observations were carried out on gardening activities and the gardens’ physical attributes. The study sample encompasses of 131 urban farmers of 22 community gardens in Subang Jaya. It was found that most of the community gardens practiced crops planting on the ground or soil base planting and dwellers in the lower income group with monthly low household income constitutes the majority (83.2%) of the respondents. Social and health benefits are the highest motivating factors for urban farmers. This study provides unprecedented insights on urban farming practices and motivations in a Malaysian setting.

  14. The Development of a Virtual Farm for Applications in Elementary Science Education

    ERIC Educational Resources Information Center

    Tarng, Wernhuar; Chang, Mei-Yu; Ou, Kuo-Liang; Yu, Kein-Fu; Hsieh, Kuen-Rong

    2012-01-01

    The goal of this study is to develop a virtual farm for educational applications. Users can play the role of a farmer by planting vegetables and raising poultry on the virtual farm to observe their growth through online learning activities. The virtual farm possesses educational and entertaining functions, and it is designed by simulating the real…

  15. Energy Economics of Farm Biogas in Cold Climates

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Pillay, Pragasen; Grimberg, Stefan; Powers, Susan E

    Anaerobic digestion of farm and dairy waste has been shown to be capital intensive. One way to improve digester economics is to co-digest high-energy substrates together with the dairy manure. Cheese whey for example represents a high-energy substrate that is generated during cheese manufacture. There are currently no quantitative tools available that predict performance of co-digestion farm systems. The goal of this project was to develop a mathematical tool that would (1) predict the impact of co-digestion and (2) determine the best use of the generated biogas for a cheese manufacturing plant. Two models were developed that separately could bemore » used to meet both goals of the project. Given current pricing structures of the most economical use of the generated biogas at the cheese manufacturing plant was as a replacement of fuel oil to generate heat. The developed digester model accurately predicted the performance of 26 farm digesters operating in the North Eastern U.S.« less

  16. Involvement of nitric oxide in the jasmonate-dependent basal defense against root-knot nematode in tomato plants.

    PubMed

    Zhou, Jie; Jia, Feifei; Shao, Shujun; Zhang, Huan; Li, Guiping; Xia, Xiaojian; Zhou, Yanhong; Yu, Jingquan; Shi, Kai

    2015-01-01

    Jasmonic acid (JA) and nitric oxide (NO) are well-characterized signaling molecules in plant defense responses. However, their roles in plant defense against root-knot nematode (RKN, Meloidogyne incognita) infection are largely unknown. In this study, we found that the transcript levels of the JA- and NO-related biosynthetic and signaling component genes were induced after RKN infection. Application of exogenous JA and sodium nitroprusside (SNP; a NO donor) significantly decreased the number of egg masses in tomato roots after RKN infection and partially alleviated RKN-induced decreases in plant fresh weight and net photosynthetic rate. These molecules also alleviated RKN-induced increases in root electrolyte leakage and membrane peroxidation. Importantly, NO scavenger partially inhibited JA-induced RKN defense. The pharmacological inhibition of JA biosynthesis significantly increased the plants' susceptibility to RKNs, which was effectively alleviated by SNP application, showing that NO may be involved in the JA-dependent RKN defense pathway. Furthermore, both JA and SNP induced increases in protease inhibitor 2 (PI2) gene expression after RKN infestation. Silencing of PI2 compromised both JA- and SNP-induced RKN defense responses, suggesting that the PI2 gene mediates JA- and NO-induced defense against RKNs. This work will be important for deepening the understanding of the mechanisms involved in basal defense against RKN attack in plants.

  17. Wound-induced endogenous jasmonates stunt plant growth by inhibiting mitosis.

    PubMed

    Zhang, Yi; Turner, John G

    2008-01-01

    When plants are repeatedly injured their growth is stunted and the size of organs such as leaves is greatly reduced. The basis of this effect is not well-understood however, even though it reduces yield of crops injured by herbivory, and produces dramatic effects exemplified in ornamental bonsai plants. We have investigated the genetic and physiological basis of this "bonsai effect" by repeatedly wounding leaves of the model plant Arabidopsis. This treatment stunted growth by 50% and increased the endogenous content of jasmonate (JA), a growth inhibitor, by seven-fold. Significantly, repeated wounding did not stunt the growth of the leaves of mutants unable to synthesise JA, or unable to respond to JA including coi1, jai3, myc2, but not jar1. The stunted growth did not result from reduced cell size, but resulted instead from reduced cell number, and was associated with reduced expression of CycB1;2. Wounding caused systemic disappearance of constitutively expressed JAZ1::GUS. Wounding also activates plant immunity. We show that a gene, 12-oxo-phytodienoate reductase, which catalyses a step in JA biosynthesis, and which we confirm is not required for defence, is however required for wound-induced stunting. Our data suggest that intermediates in the JA biosynthetic pathway activate defence, but a primary function of wound-induced JA is to stunt growth through the suppression of mitosis.

  18. Foraging and farming as niche construction: stable and unstable adaptations

    PubMed Central

    Rowley-Conwy, Peter; Layton, Robert

    2011-01-01

    All forager (or hunter–gatherer) societies construct niches, many of them actively by the concentration of wild plants into useful stands, small-scale cultivation, burning of natural vegetation to encourage useful species, and various forms of hunting, collectively termed ‘low-level food production’. Many such niches are stable and can continue indefinitely, because forager populations are usually stable. Some are unstable, but these usually transform into other foraging niches, not geographically expansive farming niches. The Epipalaeolithic (final hunter–gatherer) niche in the Near East was complex but stable, with a relatively high population density, until destabilized by an abrupt climatic change. The niche was unintentionally transformed into an agricultural one, due to chance genetic and behavioural attributes of some wild plant and animal species. The agricultural niche could be exported with modifications over much of the Old World. This was driven by massive population increase and had huge impacts on local people, animals and plants wherever the farming niche was carried. Farming niches in some areas may temporarily come close to stability, but the history of the last 11 000 years does not suggest that agriculture is an effective strategy for achieving demographic and political stability in the world's farming populations. PMID:21320899

  19. Herbivore-induced Blueberry Volatiles and Intra-plant Signaling

    PubMed Central

    Rodriguez-Saona, Cesar R.

    2011-01-01

    Herbivore-induced plant volatiles (HIPVs) are commonly emitted from plants after herbivore attack1,2. These HIPVs are mainly regulated by the defensive plant hormone jasmonic acid (JA) and its volatile derivative methyl jasmonate (MeJA)3,4,5. Over the past 3 decades researchers have documented that HIPVs can repel or attract herbivores, attract the natural enemies of herbivores, and in some cases they can induce or prime plant defenses prior to herbivore attack. In a recent paper6, I reported that feeding by gypsy moth caterpillars, exogenous MeJA application, and mechanical damage induce the emissions of volatiles from blueberry plants, albeit differently. In addition, blueberry branches respond to HIPVs emitted from neighboring branches of the same plant by increasing the levels of JA and resistance to herbivores (i.e., direct plant defenses), and by priming volatile emissions (i.e., indirect plant defenses). Similar findings have been reported recently for sagebrush7, poplar8, and lima beans9.. Here, I describe a push-pull method for collecting blueberry volatiles induced by herbivore (gypsy moth) feeding, exogenous MeJA application, and mechanical damage. The volatile collection unit consists of a 4 L volatile collection chamber, a 2-piece guillotine, an air delivery system that purifies incoming air, and a vacuum system connected to a trap filled with Super-Q adsorbent to collect volatiles5,6,10. Volatiles collected in Super-Q traps are eluted with dichloromethane and then separated and quantified using Gas Chromatography (GC). This volatile collection method was used n my study6 to investigate the volatile response of undamaged branches to exposure to volatiles from herbivore-damaged branches within blueberry plants. These methods are described here. Briefly, undamaged blueberry branches are exposed to HIPVs from neighboring branches within the same plant. Using the same techniques described above, volatiles emitted from branches after exposure to HIPVs are

  20. Contribution of urban farms to urban ecology of a developing city

    NASA Astrophysics Data System (ADS)

    Iswoyo, H.; Dariati, T.; Vale, B.; Bryant, M.

    2018-05-01

    Urban ecology has become a more popular concern as the awareness for mutual sharing between humans and other ecosystem members is increasing. This study aimed at assessing the value of urban farms in the city of Makassar, a fast-developing Indonesian city which according to its city council classification covered significant area of the city. The research employed Rapid Biodiversity Assessment (RBA) to assess quality of urban farms. The method assessed two important aspects of spaces in terms of ecology i.e. vegetation structures and vascular plants. Results showed the biodiversity of urban farms in Makassar compared to other typologies was high. Urban farms in Makassar in general have potential for ecological spots because despite they are more cultural than natural, their high plant biodiversity score, dominance of trees and less built areas make them always available for improvement to become more ecological spots.

  1. A study of biohazard protection for farming modules of lunar base CELSS.

    PubMed

    Fujii, T; Midorikawa, Y; Shiba, M; Terai, M; Omasa, K; Nitta, K

    1992-01-01

    For the Closed Ecological Life Support System (CELSS) of a manned lunar base which is planned to be built on the moon early in the 21st century, several proposed programs exist to grow vegetables inside a farming module. At the 40th IAF (Malaga, 1989) the author et al presented a proposal for supplying food and nutrients to a crew of eight members, a basic concept which is based on growing four kinds of vegetables. This paper describes measures for biohazard protection in farming modules. In this study, biohazard protection means prevention of the dispersion of plant diseases to other plant species or other portions of farming beds.

  2. Ecological and economic services provided by birds on Jamaican Blue Mountain coffee farms.

    PubMed

    Kellermann, Jherime L; Johnson, Matthew D; Stercho, Amy M; Hackett, Steven C

    2008-10-01

    Coffee farms can support significant biodiversity, yet intensification of farming practices is degrading agricultural habitats and compromising ecosystem services such as biological pest control. The coffee berry borer (Hypothenemus hampei) is the world's primary coffee pest. Researchers have demonstrated that birds reduce insect abundance on coffee farms but have not documented avian control of the berry borer or quantified avian benefits to crop yield or farm income. We conducted a bird-exclosure experiment on coffee farms in the Blue Mountains, Jamaica, to measure avian pest control of berry borers, identify potential predator species, associate predator abundance and borer reductions with vegetation complexity, and quantify resulting increases in coffee yield. Coffee plants excluded from foraging birds had significantly higher borer infestation, more borer broods, and greater berry damage than control plants. We identified 17 potential predator species (73% were wintering Neotropical migrants), and 3 primary species composed 67% of migrant detections. Average relative bird abundance and diversity and relative resident predator abundance increased with greater shade-tree cover. Although migrant predators overall did not respond to vegetation complexity variables, the 3 primary species increased with proximity to noncoffee habitat patches. Lower infestation on control plants was correlated with higher total bird abundance, but not with predator abundance or vegetation complexity. Infestation of fruit was 1-14% lower on control plants, resulting in a greater quantity of saleable fruits that had a market value of US$44-$105/ha in 2005/2006. Landscape heterogeneity in this region may allow mobile predators to provide pest control broadly, despite localized farming intensities. These results provide the first evidence that birds control coffee berry borers and thus increase coffee yield and farm income, a potentially important conservation incentive for producers.

  3. The Navajo Agricultural Projects Industry: Subsistence Farming to Corporate Agribusiness.

    ERIC Educational Resources Information Center

    Barry, Tom

    1979-01-01

    Originally designed to create small farms for individual Navajos, the irrigation project has grown into a single 110,000-acre corporate agribusiness, the land's management has fallen out of the grasp of individual Navajos, and the idea of subsistence farming has been plowed under for the planting of major money-making crops. (NQ)

  4. Energy and emergy analysis of mixed crop-livestock farming

    NASA Astrophysics Data System (ADS)

    Kuczuk, Anna; Pospolita, Janusz; Wacław, Stefan

    2017-10-01

    This paper contains substance and energy balances of mixed crop-livestock farming. The analysis involves the period between 2012 and 2015. The structure of the presentation in the paper includes: crops and their structure, details of the use of plants with a beneficial effect on soil and stocking density per 1ha of agricultural land. Cumulative energy intensity of agricultural animal and plant production was determined, which is coupled the discussion of the energy input in the production of a grain unit obtained from plant and animal production. This data was compared with the data from the literature containing examples derived from intensive and organic production systems. The environmental impact of a farm was performed on the basis of emergy analysis. Emergy fluxes were determined on the basis of renewable and non-renewable sources. As a consequence, several performance indicators were established: Emergy Yield Ratio EYR, Environmental Loading Ratio ELR and ratio of emergy from renewable sources R! . Their values were compared with the parameters characterizing other production patterns followed in agricultural production. As a consequence, conclusions were derived, in particular the ones concerning environmental sustainability of production systems in the analyzed farm.

  5. MYC2 Orchestrates a Hierarchical Transcriptional Cascade That Regulates Jasmonate-Mediated Plant Immunity in Tomato[OPEN

    PubMed Central

    Liu, Yuanyuan; Deng, Lei; Wu, Fangming; Huang, Zhuo; Zhou, Ming; Chen, Qian; Zhong, Silin

    2017-01-01

    The hormone jasmonate (JA), which functions in plant immunity, regulates resistance to pathogen infection and insect attack through triggering genome-wide transcriptional reprogramming in plants. We show that the basic helix-loop-helix transcription factor (TF) MYC2 in tomato (Solanum lycopersicum) acts downstream of the JA receptor to orchestrate JA-mediated activation of both the wounding and pathogen responses. Using chromatin immunoprecipitation sequencing (ChIP-seq) coupled with RNA sequencing (RNA-seq) assays, we identified 655 MYC2-targeted JA-responsive genes. These genes are highly enriched in Gene Ontology categories related to TFs and the early response to JA, indicating that MYC2 functions at a high hierarchical level to regulate JA-mediated gene transcription. We also identified a group of MYC2-targeted TFs (MTFs) that may directly regulate the JA-induced transcription of late defense genes. Our findings suggest that MYC2 and its downstream MTFs form a hierarchical transcriptional cascade during JA-mediated plant immunity that initiates and amplifies transcriptional output. As proof of concept, we showed that during plant resistance to the necrotrophic pathogen Botrytis cinerea, MYC2 and the MTF JA2-Like form a transcription module that preferentially regulates wounding-responsive genes, whereas MYC2 and the MTF ETHYLENE RESPONSE FACTOR.C3 form a transcription module that preferentially regulates pathogen-responsive genes. PMID:28733419

  6. Role of phytohormones in insect-specific plant reactions

    PubMed Central

    Erb, Matthias; Meldau, Stefan; Howe, Gregg A.

    2012-01-01

    The capacity to perceive and respond is integral to biological immune systems, but to what extent can plants specifically recognize and respond to insects? Recent findings suggest that plants possess surveillance systems that are able to detect general patterns of cellular damage as well as highly specific herbivore-associated cues. The jasmonate (JA) pathway has emerged as the major signaling cassette that integrates information perceived at the plant–insect interface into broad-spectrum defense responses. Specificity can be achieved via JA-independent processes and spatio-temporal changes of JA-modulating hormones, including ethylene, salicylic acid, abscisic acid, auxin, cytokinins, brassinosteroids and gibberellins. The identification of receptors and ligands and an integrative view of hormone-mediated response systems are crucial to understand specificity in plant immunity to herbivores. PMID:22305233

  7. Performance of Azolla caroliniana Willd. and Salvinia auriculata Aubl. on fish farming effluent.

    PubMed

    Toledo, J J; Penha, J

    2011-02-01

    The increasing release of untreated fish farming effluents into water courses that flow to the Pantanal wetlands in Mato Grosso (Brazil) may drive this ecosystem to eutrophication. Therefore, the growth of Azolla caroliniana Willd. and Salvinia auriculata Aubl. in fish farming effluent and their effect on its quality were evaluated for 48 days in a greenhouse. The results were compared to those obtained in a nutrient rich solution (Hoagland ½ medium). Azolla caroliniana showed lower relative growth rate in fish farming effluent (0.020 d-1) than in Hoagland ½ medium (0.029 d-1). However, S. auriculata grew slightly better in fish farming effluent (0.030 d-1) than in Hoagland ½ medium (0.025 d-1). The species apparently contributed to reduce nitrate and phosphate concentration in Hoagland ½ medium. However, in fish farming effluent, only electrical conductivity and pH were reduced by plants compared to the control without plants. Thus, A. caroliniana and S. auriculata show low potential for improving effluent quality.

  8. Organic Wheat Farming Improves Grain Zinc Concentration

    PubMed Central

    Helfenstein, Julian; Müller, Isabel; Grüter, Roman; Bhullar, Gurbir; Mandloi, Lokendra; Papritz, Andreas; Siegrist, Michael; Schulin, Rainer; Frossard, Emmanuel

    2016-01-01

    Zinc (Zn) nutrition is of key relevance in India, as a large fraction of the population suffers from Zn malnutrition and many soils contain little plant available Zn. In this study we compared organic and conventional wheat cropping systems with respect to DTPA (diethylene triamine pentaacetic acid)-extractable Zn as a proxy for plant available Zn, yield, and grain Zn concentration. We analyzed soil and wheat grain samples from 30 organic and 30 conventional farms in Madhya Pradesh (central India), and conducted farmer interviews to elucidate sociological and management variables. Total and DTPA-extractable soil Zn concentrations and grain yield (3400 kg ha-1) did not differ between the two farming systems, but with 32 and 28 mg kg-1 respectively, grain Zn concentrations were higher on organic than conventional farms (t = -2.2, p = 0.03). Furthermore, multiple linear regression analyses revealed that (a) total soil zinc and sulfur concentrations were the best predictors of DTPA-extractable soil Zn, (b) Olsen phosphate taken as a proxy for available soil phosphorus, exchangeable soil potassium, harvest date, training of farmers in nutrient management, and soil silt content were the best predictors of yield, and (c) yield, Olsen phosphate, grain nitrogen, farmyard manure availability, and the type of cropping system were the best predictors of grain Zn concentration. Results suggested that organic wheat contained more Zn despite same yield level due to higher nutrient efficiency. Higher nutrient efficiency was also seen in organic wheat for P, N and S. The study thus suggests that appropriate farm management can lead to competitive yield and improved Zn concentration in wheat grains on organic farms. PMID:27537548

  9. Organic Wheat Farming Improves Grain Zinc Concentration.

    PubMed

    Helfenstein, Julian; Müller, Isabel; Grüter, Roman; Bhullar, Gurbir; Mandloi, Lokendra; Papritz, Andreas; Siegrist, Michael; Schulin, Rainer; Frossard, Emmanuel

    2016-01-01

    Zinc (Zn) nutrition is of key relevance in India, as a large fraction of the population suffers from Zn malnutrition and many soils contain little plant available Zn. In this study we compared organic and conventional wheat cropping systems with respect to DTPA (diethylene triamine pentaacetic acid)-extractable Zn as a proxy for plant available Zn, yield, and grain Zn concentration. We analyzed soil and wheat grain samples from 30 organic and 30 conventional farms in Madhya Pradesh (central India), and conducted farmer interviews to elucidate sociological and management variables. Total and DTPA-extractable soil Zn concentrations and grain yield (3400 kg ha-1) did not differ between the two farming systems, but with 32 and 28 mg kg-1 respectively, grain Zn concentrations were higher on organic than conventional farms (t = -2.2, p = 0.03). Furthermore, multiple linear regression analyses revealed that (a) total soil zinc and sulfur concentrations were the best predictors of DTPA-extractable soil Zn, (b) Olsen phosphate taken as a proxy for available soil phosphorus, exchangeable soil potassium, harvest date, training of farmers in nutrient management, and soil silt content were the best predictors of yield, and (c) yield, Olsen phosphate, grain nitrogen, farmyard manure availability, and the type of cropping system were the best predictors of grain Zn concentration. Results suggested that organic wheat contained more Zn despite same yield level due to higher nutrient efficiency. Higher nutrient efficiency was also seen in organic wheat for P, N and S. The study thus suggests that appropriate farm management can lead to competitive yield and improved Zn concentration in wheat grains on organic farms.

  10. Soil Microbiome Is More Heterogeneous in Organic Than in Conventional Farming System

    PubMed Central

    Lupatini, Manoeli; Korthals, Gerard W.; de Hollander, Mattias; Janssens, Thierry K. S.; Kuramae, Eiko E.

    2017-01-01

    Organic farming system and sustainable management of soil pathogens aim at reducing the use of agricultural chemicals in order to improve ecosystem health. Despite the essential role of microbial communities in agro-ecosystems, we still have limited understanding of the complex response of microbial diversity and composition to organic and conventional farming systems and to alternative methods for controlling plant pathogens. In this study we assessed the microbial community structure, diversity and richness using 16S rRNA gene next generation sequences and report that conventional and organic farming systems had major influence on soil microbial diversity and community composition while the effects of the soil health treatments (sustainable alternatives for chemical control) in both farming systems were of smaller magnitude. Organically managed system increased taxonomic and phylogenetic richness, diversity and heterogeneity of the soil microbiota when compared with conventional farming system. The composition of microbial communities, but not the diversity nor heterogeneity, were altered by soil health treatments. Soil health treatments exhibited an overrepresentation of specific microbial taxa which are known to be involved in soil suppressiveness to pathogens (plant-parasitic nematodes and soil-borne fungi). Our results provide a comprehensive survey on the response of microbial communities to different agricultural systems and to soil treatments for controlling plant pathogens and give novel insights to improve the sustainability of agro-ecosystems by means of beneficial microorganisms. PMID:28101080

  11. Methyl jasmonate as a vital substance in plants.

    PubMed

    Cheong, Jong-Joo; Choi, Yang Do

    2003-07-01

    The plant floral scent methyl jasmonate (MeJA) has been identified as a vital cellular regulator that mediates diverse developmental processes and defense responses against biotic and abiotic stresses. The pleiotropic effects of MeJA have raised numerous questions about its regulation for biogenesis and mode of action. Characterization of the gene encoding jasmonic acid carboxyl methyltransferase has provided basic information on the role(s) of this phytohormone in gene-activation control and systemic long-distance signaling. Recent approaches using functional genomics and bioinformatics have identified a whole set of MeJA-responsive genes, and provide insights into how plants use volatile signals to withstand diverse and variable environments.

  12. Impact of hormonal crosstalk on plant resistance and fitness under multi-attacker conditions

    PubMed Central

    Vos, Irene A.; Moritz, Liselotte; Pieterse, Corné M. J.; Van Wees, Saskia C. M.

    2015-01-01

    The hormone salicylic acid (SA) generally induces plant defenses against biotrophic pathogens. Jasmonic acid (JA) and its oxylipin derivatives together with ethylene (ET) are generally important hormonal regulators of induced plant defenses against necrotrophic pathogens, whereas JAs together with abscisic acid (ABA) are implicated in induced plant defenses against herbivorous insects. Hormonal crosstalk between the different plant defense pathways has often been hypothesized to be a cost-saving strategy that has evolved as a means of the plant to reduce allocation costs by repression of unnecessary defenses, thereby minimizing trade-offs between plant defense and growth. However, proof for this hypothesis has not been demonstrated yet. In this study the impact of hormonal crosstalk on disease resistance and fitness of Arabidopsis thaliana when under multi-species attack was investigated. Induction of SA- or JA/ABA-dependent defense responses by the biotrophic pathogen Hyaloperonospora arabidopsidis or the herbivorous insect Pieris rapae, respectively, was shown to reduce the level of induced JA/ET-dependent defense against subsequent infection with the necrotrophic pathogen Botrytis cinerea. However, despite the enhanced susceptibility to this second attacker, no additional long-term negative effects were observed on plant fitness when plants had been challenged by multiple attackers. Similarly, when plants were grown in dense competition stands to enlarge fitness effects of induced defenses, treatment with a combination of SA and MeJA did not cause additional negative effects on plant fitness in comparison to the single MeJA treatment. Together, these data support the notion that hormonal crosstalk in plants during multi-attacker interactions allows plants to prioritize their defenses, while limiting the fitness costs associated with induction of defenses. PMID:26347758

  13. Involvement of nitric oxide in the jasmonate-dependent basal defense against root-knot nematode in tomato plants

    PubMed Central

    Zhou, Jie; Jia, Feifei; Shao, Shujun; Zhang, Huan; Li, Guiping; Xia, Xiaojian; Zhou, Yanhong; Yu, Jingquan; Shi, Kai

    2015-01-01

    Jasmonic acid (JA) and nitric oxide (NO) are well-characterized signaling molecules in plant defense responses. However, their roles in plant defense against root-knot nematode (RKN, Meloidogyne incognita) infection are largely unknown. In this study, we found that the transcript levels of the JA- and NO-related biosynthetic and signaling component genes were induced after RKN infection. Application of exogenous JA and sodium nitroprusside (SNP; a NO donor) significantly decreased the number of egg masses in tomato roots after RKN infection and partially alleviated RKN-induced decreases in plant fresh weight and net photosynthetic rate. These molecules also alleviated RKN-induced increases in root electrolyte leakage and membrane peroxidation. Importantly, NO scavenger partially inhibited JA-induced RKN defense. The pharmacological inhibition of JA biosynthesis significantly increased the plants’ susceptibility to RKNs, which was effectively alleviated by SNP application, showing that NO may be involved in the JA-dependent RKN defense pathway. Furthermore, both JA and SNP induced increases in protease inhibitor 2 (PI2) gene expression after RKN infestation. Silencing of PI2 compromised both JA- and SNP-induced RKN defense responses, suggesting that the PI2 gene mediates JA- and NO-induced defense against RKNs. This work will be important for deepening the understanding of the mechanisms involved in basal defense against RKN attack in plants. PMID:25914698

  14. Development of web-based GIS services for sustainable soil resource management at farm level

    NASA Astrophysics Data System (ADS)

    Papadopoulos, Antonis; Kolovos, Chronis; Troyanos, Yerasimos; Doula, Maria

    2017-09-01

    Modern farms situated in urban or suburban areas, include various and in most cases diverse land covers. Land uses in such farms may serve residential, structured, aesthetic and agricultural purposes, usually delimited inside the boundaries of a single property. The environmental conditions across a farm, especially if it is situated on an irregular terrain, can be highly differentiated. Managing soil resources in a small scale diverse farm environment in a holistic and sustainable way should have spatial and temporal reference and take advantage of cut-edge geospatial technologies. In present study, an 8 hectare farm with various land uses in the southern suburbs of Attica Prefecture, Greece was systematically monitored regarding its soil, water and plant resources. Almost 80% of the farm's area is covered with trees, shrubs and low vegetation planted in a mosaic of parterres. Farm data collected concerned soil and water physicochemical characteristics, plant species, topographical features, irrigation network, valves and infrastructure. All data were imported and developed in a GIS geodatabase. Furthermore, web GIS services and a mobile GIS app were developed in order to monitor, update and synchronize present status and future changes performed in the farm. Through the web services and using the mobile GIS app, the user has access to all data stored in the geodatabase and according to access rights he can view or edit the spatial entities. The user can easily make query to specific features, combine their properties with other overlaying spatial data and reach accurate decisions. The app can be downloaded and implemented in mobile devices like smartphones and tablets for extending its functionality. As proven in this study, web GIS services and mobile GIS apps constitute an attractive suite of methodologies for effective and user friendly management of natural resources at farm level.

  15. Ligand-receptor co-evolution shaped the jasmonate pathway in land plants.

    PubMed

    Monte, Isabel; Ishida, Sakiko; Zamarreño, Angel M; Hamberg, Mats; Franco-Zorrilla, José M; García-Casado, Gloria; Gouhier-Darimont, Caroline; Reymond, Philippe; Takahashi, Kosaku; García-Mina, José M; Nishihama, Ryuichi; Kohchi, Takayuki; Solano, Roberto

    2018-05-01

    The phytohormone jasmonoyl-isoleucine (JA-Ile) regulates defense, growth and developmental responses in vascular plants. Bryophytes have conserved sequences for all JA-Ile signaling pathway components but lack JA-Ile. We show that, in spite of 450 million years of independent evolution, the JA-Ile receptor COI1 is functionally conserved between the bryophyte Marchantia polymorpha and the eudicot Arabidopsis thaliana but COI1 responds to different ligands in each species. We identified the ligand of Marchantia MpCOI1 as two isomeric forms of the JA-Ile precursor dinor-OPDA (dinor-cis-OPDA and dinor-iso-OPDA). We demonstrate that AtCOI1 functionally complements Mpcoi1 mutation and confers JA-Ile responsiveness and that a single-residue substitution in MpCOI1 is responsible for the evolutionary switch in ligand specificity. Our results identify the ancestral bioactive jasmonate and clarify its biosynthetic pathway, demonstrate the functional conservation of its signaling pathway, and show that JA-Ile and COI1 emergence in vascular plants required co-evolution of hormone biosynthetic complexity and receptor specificity.

  16. Hijacking common mycorrhizal networks for herbivore-induced defence signal transfer between tomato plants

    PubMed Central

    Song, Yuan Yuan; Ye, Mao; Li, Chuanyou; He, Xinhua; Zhu-Salzman, Keyan; Wang, Rui Long; Su, Yi Juan; Luo, Shi Ming; Zeng, Ren Sen

    2014-01-01

    Common mycorrhizal networks (CMNs) link multiple plants together. We hypothesized that CMNs can serve as an underground conduit for transferring herbivore-induced defence signals. We established CMN between two tomato plants in pots with mycorrhizal fungus Funneliformis mosseae, challenged a ‘donor' plant with caterpillar Spodoptera litura, and investigated defence responses and insect resistance in neighbouring CMN-connected ‘receiver' plants. After CMN establishment caterpillar infestation on ‘donor' plant led to increased insect resistance and activities of putative defensive enzymes, induction of defence-related genes and activation of jasmonate (JA) pathway in the ‘receiver' plant. However, use of a JA biosynthesis defective mutant spr2 as ‘donor' plants resulted in no induction of defence responses and no change in insect resistance in ‘receiver' plants, suggesting that JA signalling is required for CMN-mediated interplant communication. These results indicate that plants are able to hijack CMNs for herbivore-induced defence signal transfer and interplant defence communication. PMID:24468912

  17. Application of a JA-Ile Biosynthesis Inhibitor to Methyl Jasmonate-Treated Strawberry Fruit Induces Upregulation of Specific MBW Complex-Related Genes and Accumulation of Proanthocyanidins.

    PubMed

    Delgado, Laura D; Zúñiga, Paz E; Figueroa, Nicolás E; Pastene, Edgar; Escobar-Sepúlveda, Hugo F; Figueroa, Pablo M; Garrido-Bigotes, Adrián; Figueroa, Carlos R

    2018-06-13

    Fleshy fruits are an important source of anthocyanins and proanthocyanidins (PAs), which protect plants against stress, and their consumption provides beneficial effects for human health. In strawberry fruit, the application of exogenous methyl jasmonate (MeJA) upregulates anthocyanin accumulation, although the relationship between the jasmonate pathway and anthocyanin and PA biosynthesis in fruits remains to be understood. Anthocyanin and PA accumulation is mainly regulated at the transcriptional level through R2R3-MYB and bHLH transcription factors in different plant species and organs. Here, the effect of jarin-1, a specific inhibitor of bioactive JA (jasmonoyl-isoleucine, JA-Ile) biosynthesis, on anthocyanin and PA accumulation was evaluated during strawberry ( Fragaria × ananassa ) fruit development using an in vitro ripening system for 48 h. Also, we observed the effects of MeJA and the application of jarin-1 to MeJA-treated fruits (MeJA + jarin-1 treatment). We assessed changes of expression levels for the JA-Ile and MeJA biosynthetic ( FaJAR1.2 and FaJMT ), JA signaling-related ( FaMYC2 and FaJAZ1 ), MYB-bHLH-WD40 (MBW) complex-related ( FabHLH3/33 , FaMYB9/10/11 , and repressor FaMYB1 ), and anthocyanin and PA biosynthetic (FaANS , FaUFGT , FaANR , and FaLAR ) genes. In addition, the promoter region of MBW complex-related MYB genes was isolated and sequenced. We found a higher redness of strawberry fruit skin and anthocyanin content in MeJA-treated fruits with respect to jarin-1-treated ones concomitant with an upregulation of FaANS and FaUFGT genes. Inversely, the PA content was higher in jarin-1- and MeJA + jarin-1-treated than in MeJA-treated fruits. MeJA + jarin-1 treatment resulted in an upregulation of FaANR and associated transcription factors such as FabHLH33 and FaMYB9/11 along with FaJMT and FaJAR1.2 . Finally, we found JA-responsive elements in the promoter regions of FaMYB1/9/10/11 genes. It is proposed that PA biosynthesis-related genes

  18. High School Beef Farm is Career Education Center

    ERIC Educational Resources Information Center

    Herren, Ray

    1976-01-01

    The beef farm at Gaylesville High School, Gaylesville, Alabama, has provided an opportunity for students to gain work experience in a variety of areas including construction, animal care, and packing plant procedures. (LH)

  19. Molecular farming on the rise--GMO regulators still walking a tightrope.

    PubMed

    Spök, Armin

    2007-02-01

    Recent increases in EU commercial and academic activities in molecular farming, and the proximity to market-stage of the first plant-made pharmaceuticals, represent a call to action for EU regulators. Drawing on the North American debate on molecular farming, it will be argued that both the rationale and the risks of molecular farming will differ significantly from those of first generation GM crops. Based on these differences, the suitability of the existing regulatory frameworks, which were developed in response to the arrival of earlier products, is discussed, and specific options for adapting the already complex EU regulatory system to cater for molecular farming are examined.

  20. Capital Strategy in Diversification Farming Efforts Using SWOT Analysis

    NASA Astrophysics Data System (ADS)

    Damanhuri; Setyohadi, D. P. S.; Utami, M. M. D.; Kurnianto, M. F.; Hariono, B.

    2018-01-01

    Wetland farm diversification program in the district of Bojonegoro, Tulungagung, and Ponorogo can not provide an optimal contribution to the income of farmers caused because farmers are not able to cultivate high value-added commodities due to limited capital. This study aims to identify the characteristics of farming, capital pattern, stakeholder role, to analyze farming to know the pattern of planting suggestions and prospects, and to formulate capital facilitation strategy. Farming capital is obtained through loans in financial institutions with different patterns. Small farmers tend to utilize savings and credit cooperatives, microcredit, and loan sharks, while farmers with large wetland holdings tend to utilize commercial banks. P enelitian using descriptive method of farming profit analysis, and SWOT. The government through the banking institutions have provided much facilitation in the form of low-interest loans with flexible payment method. The generic strategy of selected capital facilitation is to empower farmers through farmer groups who have the capability in managing the capital needs of their members.

  1. Evaluation of ecofriendly management practices of french beanrust (Uromyces appendiculatus) in organic farming system

    NASA Astrophysics Data System (ADS)

    Chhetry, G. K. N.; Mangang, H. C.

    2012-09-01

    Organic farming system emphasises on sustainable development of agriculture. The traditional agriculture system was much akin to the organic system but modernization of agriculture made a shift to this trend. The north east region of India is potential organic farming sites. Most of the farming systems are traditional and are organic by default; however crops in organic farming are prone to many fungal diseases. Hence for validation of the impact of organic practices on the disease development of plants, a study has been conducted for three years under natural environmental conditions on bean rust (Uromyces appendiculatus). Study includes ecofriendly practices like: plant extract treatment, intercropping of beans with maize, organic manure application, influence of cropping season and Trichoderma treatment. Rust is a major prevalent disease in the cultivation of beans as in other parts of the world. Detailed study of the disease in the organic environment and the impact of various treatments and agricultural agronomic practices would help in validation of the practices for the management of the disease in the organic farming system. In our study for three consecutive years it has been revealed that the practices of the traditional farmers likeplant extract application, intercropping, and manure application were found to have significant positive effects in reducing rust development in the bean fields. The treatment of farm yard manure resulted in development of lesser area under disease progress curve. The plant extract of Artemisia vulgaris has marked positive impact on reducing rust disease parameters. Foliar application of Trichoderma reduces the disease parameters of rust. This study would enhance information in understanding the impact of organic farming system on bean rust and would help in validitation of sustainable agricultural practices for use in organic farming system.

  2. Roles of jasmonate signalling in plant inflorescence and flower development.

    PubMed

    Yuan, Zheng; Zhang, Dabing

    2015-10-01

    Development of inflorescences and flowers in plants is controlled by the combined action of environmental and genetic signals. Investigations reveal that the phytohormone jasmonate (JA) plays a critical function in plant reproduction such as male fertility, sex determination and seed maturation. Here, we review recent progress on JA synthesis, signalling, the interplay between JAs and other hormones, and regulatory network of JA in controlling the development of inflorescence, flower and the male organ. The conserved and diversified roles of JAs in meristem transition and specification of flower organ identity and number, and multiple regulatory networks of JAs in stamen development are highlighted. Further, this review provides perspectives on future research endeavors to elucidate mechanisms underlying JAs homeostasis and transport during plant reproductive development. Copyright © 2015 Elsevier Ltd. All rights reserved.

  3. Plant-mediated effects on an insect-pathogen interaction vary with intraspecific genetic variation in plant defences.

    PubMed

    Shikano, Ikkei; Shumaker, Ketia L; Peiffer, Michelle; Felton, Gary W; Hoover, Kelli

    2017-04-01

    Baculoviruses are food-borne microbial pathogens that are ingested by insects on contaminated foliage. Oxidation of plant-derived phenolics, activated by insect feeding, can directly interfere with infections in the gut. Since phenolic oxidation is an important component of plant resistance against insects, baculoviruses are suggested to be incompatible with plant defences. However, plants among and within species invest differently in a myriad of chemical and physical defences. Therefore, we hypothesized that among eight soybean genotypes, some genotypes would be able to maintain both high resistance against an insect pest and high efficacy of a baculovirus. Soybean constitutive (non-induced) and jasmonic acid (JA)-induced (anti-herbivore response) resistance was measured against the fall armyworm Spodoptera frugiperda (weight gain, leaf consumption and utilization). Indicators of phenolic oxidation were measured as foliar phenolic content and peroxidase activity. Levels of armyworm mortality inflicted by baculovirus (SfMNPV) did not vary among soybean genotypes when the virus was ingested with non-induced foliage. Ingestion of the virus on JA-induced foliage reduced armyworm mortality, relative to non-induced foliage, on some soybean genotypes. Baculovirus efficacy was lower when ingested with foliage that contained higher phenolic content and defensive properties that reduced armyworm weight gain and leaf utilization. However, soybean genotypes that defended the plant by reducing consumption rate and strongly deterred feeding upon JA-induction did not reduce baculovirus efficacy, indicating that these defences may be more compatible with baculoviruses to maximize plant protection. Differential compatibility of defence traits with the third trophic level highlights an important cost/trade-off associated with plant defence strategies.

  4. Induction of direct and indirect plant responses by jasmonic acid, low spider mite densities, or a combination of jasmonic acid treatment and spider mite infestation.

    PubMed

    Gols, Rieta; Roosjen, Mara; Dijkman, Herman; Dicke, Marcel

    2003-12-01

    Jasmonic acid (JA) and the octadecanoid pathway are involved in both induced direct and induced indirect plant responses. In this study, the herbivorous mite, Tetranychus urticae, and its predator, Phytoseiulus persimilis, were given a choice between Lima bean plants induced by JA or spider mites and uninduced control plants. Infestation densities resulting in the induction of predator attractants were much lower than thus far assumed, i.e., predatory mites were significantly attracted to plants that were infested for 2 days with only one or four spider mites per plant. Phytoseiulus persimilis showed a density-dependent response to volatiles from plants that were infested with different numbers of spider mites. Similarly, treating plants with increasing concentrations of JA also led to increased attraction of P. persimilis. Moreover, the duration of spider mite infestation was positively correlated with the proportion of predators that were attracted to mite-infested plants. A pretreatment of the plants with JA followed by a spider mite infestation enhanced the attraction of P. persimilis to plant volatiles compared to attraction to volatiles from plants that were only infested with spider mites and did not receive a pretreatment with JA. The herbivore, T. urticae preferred leaf tissue that previously had been infested with conspecifics to uninfested leaf tissue. In the case of choice tests with JA-induced and control leaf tissue, spider mites slightly preferred control leaf tissue. When spider mites were given a choice between leaf discs induced by JA and leaf discs damaged by spider mite feeding, they preferred the latter. The presence of herbivore induced chemicals and/or spider mite products enhanced settlement of the mites, whereas treatment with JA seemed to impede settlement.

  5. Transcriptome Analysis in Haematococcus pluvialis: Astaxanthin Induction by Salicylic Acid (SA) and Jasmonic Acid (JA).

    PubMed

    Gao, Zhengquan; Li, Yan; Wu, Guanxun; Li, Guoqiang; Sun, Haifeng; Deng, Suzhen; Shen, Yicheng; Chen, Guoqiang; Zhang, Ruihao; Meng, Chunxiao; Zhang, Xiaowen

    2015-01-01

    Haematococcus pluvialis is an astaxanthin-rich microalga that can increase its astaxanthin production by salicylic acid (SA) or jasmonic acid (JA) induction. The genetic transcriptome details of astaxanthin biosynthesis were analyzed by exposing the algal cells to 25 mg/L of SA and JA for 1, 6 and 24 hours, plus to the control (no stress). Based on the RNA-seq analysis, 56,077 unigenes (51.7%) were identified with functions in response to the hormone stress. The top five identified subcategories were cell, cellular process, intracellular, catalytic activity and cytoplasm, which possessed 5600 (~9.99%), 5302 (~9.45%), 5242 (~9.35%), 4407 (~7.86%) and 4195 (~7.48%) unigenes, respectively. Furthermore, 59 unigenes were identified and assigned to 26 putative transcription factors (TFs), including 12 plant-specific TFs. They were likely associated with astaxanthin biosynthesis in Haematococcus upon SA and JA stress. In comparison, the up-regulation of differential expressed genes occurred much earlier, with higher transcript levels in the JA treatment (about 6 h later) than in the SA treatment (beyond 24 h). These results provide valuable information for directing metabolic engineering efforts to improve astaxanthin biosynthesis in H. pluvialis.

  6. Role of plant hormones in plant defence responses.

    PubMed

    Bari, Rajendra; Jones, Jonathan D G

    2009-03-01

    Plant hormones play important roles in regulating developmental processes and signaling networks involved in plant responses to a wide range of biotic and abiotic stresses. Significant progress has been made in identifying the key components and understanding the role of salicylic acid (SA), jasmonates (JA) and ethylene (ET) in plant responses to biotic stresses. Recent studies indicate that other hormones such as abscisic acid (ABA), auxin, gibberellic acid (GA), cytokinin (CK), brassinosteroids (BR) and peptide hormones are also implicated in plant defence signaling pathways but their role in plant defence is less well studied. Here, we review recent advances made in understanding the role of these hormones in modulating plant defence responses against various diseases and pests.

  7. A case study of precision farming for nutrient management of corn

    NASA Astrophysics Data System (ADS)

    Blanco, Alfonso; Hunt, Ray; Gomez, Richard B.; Roper, William E.

    2003-08-01

    Precision farming relies on the cost effectiveness of collecting and interpreting data, which describes the variations of agricultural conditions such as crop stresses, nutrient deficiencies, water stresses, or pest infestation. Hyperspectral remote sensing from satellites and airborne sensors can be a way to obtain data needed to develop site-specific farming management strategies. The primary objective of the hyperspectral applications in precision farming is to provide farmers with a technology, which can detect specific crop conditions that can be used to program variable-rate applications. Applications of water, pesticides, and fertilizer can be tailored to the needs of the agricultural crops, based on the conditions reflected on the imagery. This paper presents an experimental study performed in Beltsville, Maryland for assessing the plant density and nutrient uptake of corn using a simple photographic method from a model airplane versus obtaining hyperspectral imagery from an airborne sensor. The hyperspectral sensor utilized in this study was the AISA sensor. These remote sensors can measure the temperature of plants; or to be more specific, they can measure how much energy plants emit at the visible and near-infrared wavelengths of the spectrum, such as water and vegetation.

  8. Ecological trade-offs between jasmonic acid-dependent direct and indirect plant defences in tritrophic interactions

    PubMed Central

    Wei, Jianing; Wang, Lizhong; Zhao, Jiuhai; Li, Chuanyou; Ge, Feng; Kang, Le

    2011-01-01

    Recent studies on plants genetically modified in jasmonic acid (JA) signalling support the hypothesis that the jasmonate family of oxylipins plays an important role in mediating direct and indirect plant defences. However, the interaction of two modes of defence in tritrophic systems is largely unknown. In this study, we examined the preference and performance of a herbivorous leafminer (Liriomyza huidobrensis) and its parasitic wasp (Opius dissitus) on three tomato genotypes: a wild-type (WT) plant, a JA biosynthesis (spr2) mutant, and a JA-overexpression 35S::prosys plant. Their proteinase inhibitor production and volatile emission were used as direct and indirect defence factors to evaluate the responses of leafminers and parasitoids. Here, we show that although spr2 mutant plants are compromised in direct defence against the larval leafminers and in attracting parasitoids, they are less attractive to adult flies compared with WT plants. Moreover, in comparison to other genotypes, the 35S::prosys plant displays greater direct and constitutive indirect defences, but reduced success of parasitism by parasitoids. Taken together, these results suggest that there are distinguished ecological trade-offs between JA-dependent direct and indirect defences in genetically modified plants whose fitness should be assessed in tritrophic systems and under natural conditions. PMID:21039561

  9. A fungal endophyte helps plants to tolerate root herbivory through changes in gibberellin and jasmonate signaling.

    PubMed

    Cosme, Marco; Lu, Jing; Erb, Matthias; Stout, Michael Joseph; Franken, Philipp; Wurst, Susanne

    2016-08-01

    Plant-microbe mutualisms can improve plant defense, but the impact of root endophytes on below-ground herbivore interactions remains unknown. We investigated the effects of the root endophyte Piriformospora indica on interactions between rice (Oryza sativa) plants and its root herbivore rice water weevil (RWW; Lissorhoptrus oryzophilus), and how plant jasmonic acid (JA) and GA regulate this tripartite interaction. Glasshouse experiments with wild-type rice and coi1-18 and Eui1-OX mutants combined with nutrient, jasmonate and gene expression analyses were used to test: whether RWW adult herbivory above ground influences subsequent damage caused by larval herbivory below ground; whether P. indica protects plants against RWW; and whether GA and JA signaling mediate these interactions. The endophyte induced plant tolerance to root herbivory. RWW adults and larvae acted synergistically via JA signaling to reduce root growth, while endophyte-elicited GA biosynthesis suppressed the herbivore-induced JA in roots and recovered plant growth. Our study shows for the first time the impact of a root endophyte on plant defense against below-ground herbivores, adds to growing evidence that induced tolerance may be an important root defense, and implicates GA as a signal component of inducible plant tolerance against biotic stress. © 2016 The Authors. New Phytologist © 2016 New Phytologist Trust.

  10. Student Outcomes from Experiential Learning on a Student-Run Certified Organic Farm

    ERIC Educational Resources Information Center

    Reeve, Jennifer R.; Hall, Kelsey; Kalkman, Carol

    2014-01-01

    The USU Student Organic Farm was initiated in April 2008 on 1 acre (0.4 ha) of land at Utah State University (USU). Students plant, weed, harvest, and market the produce on campus. Proceeds support the student farm in terms of operating expenses, equipment purchases, and modest student stipends. Since 2009, the students have operated a community…

  11. Human Norovirus and Its Surrogates Induce Plant Immune Response in Arabidopsis thaliana and Lactuca sativa.

    PubMed

    Markland, Sarah M; Bais, Harsh; Kniel, Kalmia E

    2017-08-01

    Human norovirus is the leading cause of foodborne illness worldwide with the majority of outbreaks linked to fresh produce and leafy greens. It is essential that we thoroughly understand the type of relationship and interactions that take place between plants and human norovirus to better utilize control strategies to reduce transmission of norovirus in the field onto plants harvested for human consumption. In this study the expression of gene markers for the salicylic acid (SA) and jasmonic acid (JA) plant defense pathways was measured and compared in romaine lettuce (Lactuca sativa) and Arabidopsis thaliana Col-0 plants that were inoculated with Murine Norovirus-1, Tulane Virus, human norovirus GII.4, or Hank's Balanced Salt Solution (control). Genes involving both the SA and JA pathways were expressed in both romaine lettuce and A. thaliana for all three viruses, as well as controls. Studies, including gene expression of SA- and JA-deficient A. thaliana mutant lines, suggest that the JA pathway is more likely involved in the plant immune response to human norovirus. This research provides the first pieces of information regarding how foodborne viruses interact with plants in the preharvest environment.

  12. Are large farms more efficient? Tenure security, farm size and farm efficiency: evidence from northeast China

    NASA Astrophysics Data System (ADS)

    Zhou, Yuepeng; Ma, Xianlei; Shi, Xiaoping

    2017-04-01

    How to increase production efficiency, guarantee grain security, and increase farmers' income using the limited farmland is a great challenge that China is facing. Although theory predicts that secure property rights and moderate scale management of farmland can increase land productivity, reduce farm-related costs, and raise farmer's income, empirical studies on the size and magnitude of these effects are scarce. A number of studies have examined the impacts of land tenure or farm size on productivity or efficiency, respectively. There are also a few studies linking farm size, land tenure and efficiency together. However, to our best knowledge, there are no studies considering tenure security and farm efficiency together for different farm scales in China. In addition, there is little study analyzing the profit frontier. In this study, we particularly focus on the impacts of land tenure security and farm size on farm profit efficiency, using farm level data collected from 23 villages, 811 households in Liaoning in 2015. 7 different farm scales have been identified to further represent small farms, median farms, moderate-scale farms, and large farms. Technical efficiency is analyzed with stochastic frontier production function. The profit efficiency is regressed on a set of explanatory variables which includes farm size dummies, land tenure security indexes, and household characteristics. We found that: 1) The technical efficiency scores for production efficiency (average score = 0.998) indicate that it is already very close to the production frontier, and thus there is little room to improve production efficiency. However, there is larger space to raise profit efficiency (average score = 0.768) by investing more on farm size expansion, seed, hired labor, pesticide, and irrigation. 2) Farms between 50-80 mu are most efficient from the viewpoint of profit efficiency. The so-called moderate-scale farms (100-150 mu) according to the governmental guideline show no

  13. Soil management practices under organic farming

    NASA Astrophysics Data System (ADS)

    Aly, Adel; Chami Ziad, Al; Hamdy, Atef

    2015-04-01

    Organic farming methods combine scientific knowledge of ecology and modern technology with traditional farming practices based on naturally occurring biological processes. Soil building practices such as crop rotations, intercropping, symbiotic associations, cover crops, organic fertilizers and minimum tillage are central to organic practices. Those practices encourage soil formation and structure and creating more stable systems. In farm nutrient and energy cycling is increased and the retentive abilities of the soil for nutrients and water are enhanced. Such management techniques also play an important role in soil erosion control. The length of time that the soil is exposed to erosive forces is decreased, soil biodiversity is increased, and nutrient losses are reduced, helping to maintain and enhance soil productivity. Organic farming as systematized and certifiable approach for agriculture, there is no surprise that it faces some challenges among both farmers and public sector. This can be clearly demonstrated particularly in the absence of the essential conditions needed to implement successfully the soil management practices like green manure and composting to improve soil fertility including crop rotation, cover cropping and reduced tillage. Those issues beside others will be fully discussed highlighting their beneficial impact on the environmental soil characteristics. Keywords: soil fertility, organic matter, plant nutrition

  14. The effects of surface-applied jasmonic and salicylic acids on caterpillar growth and damage to tomato plants

    Treesearch

    Aaron L. Iverson; Louis R. Iverson; Steve Eshita

    2001-01-01

    We tested the role of salicylic acid (SA) and jasmonic acid (JA) in altering the tomato plant's defense against herbivory by tobacco hornworm. Treatments of SA or JA were topically applied to tomato plants, hornworm consumption was allowed to proceed for 12 days, and harvest analyses were performed Measurements taken included a subjective plant rating (1-10 score...

  15. Jasmonate Regulates Plant Responses to Postsubmergence Reoxygenation through Transcriptional Activation of Antioxidant Synthesis1

    PubMed Central

    Dai, Yang-Shuo; Xie, Li-Juan; Yu, Lu-Jun; Zhou, Ying; Lai, Yong-Xia; Yang, Yi-Cong; Xu, Le; Chen, Qin-Fang

    2017-01-01

    Submergence induces hypoxia in plants; exposure to oxygen following submergence, termed reoxygenation, produces a burst of reactive oxygen species. The mechanisms of hypoxia sensing and signaling in plants have been well studied, but how plants respond to reoxygenation remains unclear. Here, we show that reoxygenation in Arabidopsis (Arabidopsis thaliana) involves rapid accumulation of jasmonates (JAs) and increased transcript levels of JA biosynthesis genes. Application of exogenous methyl jasmonate improved tolerance to reoxygenation in wild-type Arabidopsis; also, mutants deficient in JA biosynthesis and signaling were very sensitive to reoxygenation. Moreover, overexpression of the transcription factor gene MYC2 enhanced tolerance to posthypoxic stress, and myc2 knockout mutants showed increased sensitivity to reoxygenation, indicating that MYC2 functions as a key regulator in the JA-mediated reoxygenation response. MYC2 transcriptionally activates members of the VITAMIN C DEFECTIVE (VTC) and GLUTATHIONE SYNTHETASE (GSH) gene families, which encode rate-limiting enzymes in the ascorbate and glutathione synthesis pathways. Overexpression of VTC1 and GSH1 in the myc2-2 mutant suppressed the posthypoxic hypersensitive phenotype. The JA-inducible accumulation of antioxidants may alleviate oxidative damage caused by reoxygenation, improving plant survival after submergence. Taken together, our findings demonstrate that JA signaling interacts with the antioxidant pathway to regulate reoxygenation responses in Arabidopsis. PMID:28082717

  16. Economic Indicators of the Farm Sector. State Financial Summary, 1984.

    ERIC Educational Resources Information Center

    Economic Research Service (USDA), Washington, DC.

    Crop production in 1984, particularly for spring-planted crops, returned to near-normal levels from the much reduced output of 1983 when government programs curbed planted acreage and drought depressed yields. The top five states in net farm income were California, at $3.5 billion; Iowa, $2.2 billion; Florida, $1.8 billion; and Illinois, $1.7…

  17. SIMULATION OF CARBON DIOXIDE EMISSIONS FROM DAIRY FARMS TO ASSESS GREENHOUSE GAS REDUCTION STRATEGIES

    USDA-ARS?s Scientific Manuscript database

    Farming practices can have a large impact on the soil carbon cycle and the resulting net emission of greenhouse gases including carbon dioxide (CO**2), methane and nitrous oxide. Primary sources of CO**2 emission on dairy farms are soil, plant, and animal respiration with smaller contributions from ...

  18. Chicken Farming in Grassland Increases Environmental Sustainability and Economic Efficiency

    PubMed Central

    Liu, Meizhen; Wang, Bingxue; Osborne, Colin P.; Jiang, Gaoming

    2013-01-01

    Background Grassland degradation caused by overgrazing poses a threat to both animal husbandry and environmental sustainability in most semi-arid areas especially north China. Although the Chinese Government has made huge efforts to restore degraded grasslands, a considerable attempt has unfortunately failed due to an inadequate consideration of economic benefits to local communities. Methodology/Principal Findings A controlled field experiment was conducted to test our hypothesis that utilizing natural grasslands as both habitat and feed resources for chickens and replacing the traditional husbandry system with chicken farming would increase environmental sustainability and raise income. Aboveground plant biomass elevated from 25 g m−2 for grazing sheep to 84 g m−2 for chicken farming. In contrast to the fenced (unstocked) grassland, chicken farming did not significantly decrease aboveground plant biomass, but did increase the root biomass by 60% (p<0.01). Compared with traditional sheep grazing, chicken farming significantly improved soil surface water content (0–10 cm), from 5% to 15%. Chicken farming did not affect the soil bulk density, while the traditional sheep grazing increased the soil bulk density in the 0–10 cm soil layer by 35% of the control (p<0.05). Most importantly, the economic income of local herdsmen has been raised about six times compared with the traditional practice of raising sheep. Ecologically, such an innovative solution allowed large degraded grasslands to naturally regenerate. Grasslands also provided a high quality organic poultry product which could be marketed in big cities. Conclusion/Significance Chicken farming is an innovative alternative strategy for increasing environmental sustainability and economic income, rather than a challenge to the traditional nomadic pastoral system. Our approach might be technically applicable to other large degraded grasslands of the world, especially in China. PMID:23372678

  19. Chicken farming in grassland increases environmental sustainability and economic efficiency.

    PubMed

    Liu, Meizhen; Wang, Bingxue; Osborne, Colin P; Jiang, Gaoming

    2013-01-01

    Grassland degradation caused by overgrazing poses a threat to both animal husbandry and environmental sustainability in most semi-arid areas especially north China. Although the Chinese Government has made huge efforts to restore degraded grasslands, a considerable attempt has unfortunately failed due to an inadequate consideration of economic benefits to local communities. A controlled field experiment was conducted to test our hypothesis that utilizing natural grasslands as both habitat and feed resources for chickens and replacing the traditional husbandry system with chicken farming would increase environmental sustainability and raise income. Aboveground plant biomass elevated from 25 g m(-2) for grazing sheep to 84 g m(-2) for chicken farming. In contrast to the fenced (unstocked) grassland, chicken farming did not significantly decrease aboveground plant biomass, but did increase the root biomass by 60% (p<0.01). Compared with traditional sheep grazing, chicken farming significantly improved soil surface water content (0-10 cm), from 5% to 15%. Chicken farming did not affect the soil bulk density, while the traditional sheep grazing increased the soil bulk density in the 0-10 cm soil layer by 35% of the control (p<0.05). Most importantly, the economic income of local herdsmen has been raised about six times compared with the traditional practice of raising sheep. Ecologically, such an innovative solution allowed large degraded grasslands to naturally regenerate. Grasslands also provided a high quality organic poultry product which could be marketed in big cities. Chicken farming is an innovative alternative strategy for increasing environmental sustainability and economic income, rather than a challenge to the traditional nomadic pastoral system. Our approach might be technically applicable to other large degraded grasslands of the world, especially in China.

  20. White meat-Green farm: case study of Brinson Farms

    USDA-ARS?s Scientific Manuscript database

    Comprehensive on-farm resource utilization and renewable energy generation at the farm scale are not new concepts. However, truly encompassing implementation of these ideals is lacking. Brinson Farms operates 10 commercial broiler houses. The farm generates heat for its houses using biomass boile...

  1. The effect of organic farming systems on species diversity

    NASA Astrophysics Data System (ADS)

    Leksono, Amin Setyo

    2017-11-01

    Organic farming systems have been well known to support the diversity of a wide range of taxa, including microorganisms, arable flora, invertebrates, birds, and mammals, which benefit from organic management leading to increases in abundance and/or species richness. The objective of this paper is to review the effect of organic farming on species diversity reported in several articles and compare this with the current study in Gondanglegi, Malang. A review of several studies showed that organic farming systems have been reported to increase species diversity, including that of mammals, birds, arthropods, vascular plants and arbuscular mycorrhizal fungi. The researchers about arthropod groups consisted of carabid beetles, butterflies, wasps, predators, and bees. Agricultural landscape, habitat type, farming system, landscape composition and connectivity all contribute to explaining species biodiversity and richness. Moreover, based on current and relevant studies, the results showed that the application of refugia blocks has increased arthropod diversity and composition.

  2. Certified safe farm: identifying and removing hazards on the farm.

    PubMed

    Rautiainen, R H; Grafft, L J; Kline, A K; Madsen, M D; Lange, J L; Donham, K J

    2010-04-01

    This article describes the development of the Certified Safe Farm (CSF) on-farm safety review tools, characterizes the safety improvements among participating farms during the study period, and evaluates differences in background variables between low and high scoring farms. Average farm review scores on 185 study farms improved from 82 to 96 during the five-year study (0-100 scale, 85 required for CSF certification). A total of 1292 safety improvements were reported at an estimated cost of $650 per farm. A wide range of improvements were made, including adding 9 rollover protective structures (ROPS), 59 power take-off (PTO) master shields, and 207 slow-moving vehicle (SMV) emblems; improving lighting on 72 machines: placing 171 warning decals on machinery; shielding 77 moving parts; locking up 17 chemical storage areas, adding 83 lockout/tagout improvements; and making general housekeeping upgrades in 62 farm buildings. The local, trained farm reviewers and the CSF review process overall were well received by participating farmers. In addition to our earlier findings where higher farm review scores were associated with lower self-reported health outcome costs, we found that those with higher farm work hours, younger age, pork production in confinement, beef production, poultry production, and reported exposure to agrichemicals had higher farm review scores than those who did not have these characteristics. Overall, the farm review process functioned as expected. encouraging physical improvements in the farm environment, and contributing to the multi-faceted CSF intervention program.

  3. Farming systems and sanitary problems in mountain cattle farms.

    PubMed

    Bernúes, A; Manrique, E; Maza, M T

    1994-01-01

    On the basis of concepts established by ecopathology and the systems theory, certain aspects of the 'Ecosanitary System', which forms part of the 'Farming System', were studied. Multivariant statistical methods were used to analyze and classify 69 mountain cattle farms into different types and to establish relationships between variables relating to pathological problems and others relating to aspects of production and farm structure. Stable mastitis characterized farms with a higher milk production, more intensive farming and greater hygiene measures. The pattern of diarrhoea in the calves was similar. Problems relating to reproduction and calving were more characteristic of traditional, small farms.

  4. Antagonism between phytohormone signalling underlies the variation in disease susceptibility of tomato plants under elevated CO2

    PubMed Central

    Zhang, Shuai; Li, Xin; Sun, Zenghui; Shao, Shujun; Hu, Lingfei; Ye, Meng; Zhou, Yanhong; Xia, Xiaojian; Yu, Jingquan; Shi, Kai

    2015-01-01

    Increasing CO2 concentrations ([CO2]) have the potential to disrupt plant–pathogen interactions in natural and agricultural ecosystems, but the research in this area has often produced conflicting results. Variations in phytohormone salicylic acid (SA) and jasmonic acid (JA) signalling could be associated with variations in the responses of pathogens to plants grown under elevated [CO2]. In this study, interactions between tomato plants and three pathogens with different infection strategies were compared. Elevated [CO2] generally favoured SA biosynthesis and signalling but repressed the JA pathway. The exposure of plants to elevated [CO2] revealed a lower incidence and severity of disease caused by tobacco mosaic virus (TMV) and by Pseudomonas syringae, whereas plant susceptibility to necrotrophic Botrytis cinerea increased. The elevated [CO2]-induced and basal resistance to TMV and P. syringae were completely abolished in plants in which the SA signalling pathway nonexpressor of pathogenesis-related genes 1 (NPR1) had been silenced or in transgenic plants defective in SA biosynthesis. In contrast, under both ambient and elevated [CO2], the susceptibility to B. cinerea highly increased in plants in which the JA signalling pathway proteinase inhibitors (PI) gene had been silenced or in a mutant affected in JA biosynthesis. However, plants affected in SA signalling remained less susceptible to this disease. These findings highlight the modulated antagonistic relationship between SA and JA that contributes to the variation in disease susceptibility under elevated [CO2]. This information will be critical for investigating how elevated CO2 may affect plant defence and the dynamics between plants and pathogens in both agricultural and natural ecosystems. PMID:25657213

  5. North Carolina farm women: opportunities for support and farm-related education.

    PubMed

    Tutor-Marcom, Robin; Bruce, Jacklyn; Greer, Annette

    2014-01-01

    The stress that farming visits upon male farmers has been acknowledged for decades. Stress- and work-related injuries among nonmigrant farm women is well documented from 1980 through the mid-1990s. A void of literature concerning nonmigrant farm women exists since that time. One possible explanation for this deficit is that United States Department of Agriculture Census of Agriculture data only consider the contributions of women on the farm if they are reported as farm operators. From 2002 to 2007, the number of women farm operators in North Carolina (NC) increased by 3%, and currently 13% of the state's farms are operated by women. These numbers emphasize the importance of understanding the self-perceived needs of women farmers. A qualitative research project was conducted to investigate the social-emotional needs of NC farm women. Semistructured interviews were conducted with 15 women with whom the NC Agromedicine Institute had previously worked in collaboration on farm health and safety. Key themes from interviews were (1) chameleonic, (2) inseparable connectedness, (3) farm sword, (4) women of a feather, and (5) one size doesn't fit all. Participants reported multiple roles, difficulty separating from the farm, preferring the farm over any other place, and viewing themselves as misperceived farm professionals. Participants need opportunities to interact with other farm women for support and sharing farm-management techniques. Future study recommendations include (1) inventory existing programs for farm women; (2) further investigate the support and educational needs of farm women; and (3) examine how farm women are perceived by nonfarm individuals.

  6. Jasmonate action in plant growth and development.

    PubMed

    Huang, Huang; Liu, Bei; Liu, Liangyu; Song, Susheng

    2017-03-01

    Phytohormones, including jasmonates (JAs), gibberellin, ethylene, abscisic acid, and auxin, integrate endogenous developmental cues with environmental signals to regulate plant growth, development, and defense. JAs are well- recognized lipid-derived stress hormones that regulate plant adaptations to biotic stresses, including herbivore attack and pathogen infection, as well as abiotic stresses, including wounding, ozone, and ultraviolet radiation. An increasing number of studies have shown that JAs also have functions in a remarkable number of plant developmental events, including primary root growth, reproductive development, and leaf senescence. Since the 1980s, details of the JA biosynthesis pathway, signaling pathway, and crosstalk during plant growth and development have been elucidated. Here, we summarize recent advances and give an updated overview of JA action and crosstalk in plant growth and development. © The Author 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com.

  7. Response of Plutella xylostella and its parasitoid Cotesia plutellae to volatile compounds.

    PubMed

    Ibrahim, Mohamed A; Nissinen, Anne; Holopainen, Jarmo K

    2005-09-01

    The effects of limonene, a mixture of limonene + carvone (1:1, v/v), and methyl jasmonate (MeJA) on diamondback moth (DBM) (Plutella xylostella L.) oviposition, larval feeding, and the behavior of its larval parasitoid Cotesia plutellae (Kurdjumov) with cabbage (Brassica oleracea L. ssp. capitata, cvs. Rinda and Lennox) and broccoli (B. oleracea subsp. Italica cv Lucky) were tested. Limonene showed no deterrent effect on DBM when plants were sprayed with or exposed to limonene, although there was a cultivar difference. A mixture of limonene and carvone released from vermiculite showed a significant repellent effect, reducing the number of eggs laid on the cabbages. MeJA treatment reduced the relative growth rate (RGR) of larvae on cv Lennox leaves. In Y-tube olfactometer tests, C. plutellae preferred the odors of limonene and MeJA to filtered air. In cv Lennox, the parasitoid preferred DBM-damaged plants with limonene to such plants without limonene. C. plutellae females were repelled by the mixture of limonene + carvone. In both cultivars, exogenous MeJA induced the emission of the sesquiterpene (E,E)-alpha-farnesene, the homoterpene (E)-4,8-dimethyl-1,3,7-nonatriene (DMNT), and green leaf volatile (Z)-3-hexenyl acetate + octanal. The attractive effect of limonene and MeJA predicts that these two compounds can be used in sustainable plant protection strategies in organic farming.

  8. Biogenerative life-support system: Farming on the Moon

    NASA Astrophysics Data System (ADS)

    Salisbury, Frank B.

    Plants can be used to recycle food, oxygen, and water in a closed habitat (e.g., on the moon, Mars, or in a space craft. A variety of crops might be grown, probably in underground growth units to avoid harmful radiation and micrometeorites. Artificial light will be necessary although some sunlight might be brought in via fiber optics. Transpired water will be condensed in coils exposed to space and shaded from sunlight. Oxygen and CO 2 levels will be maintained by controlling photosynthesis and waste oxidation. Plants will be grown hydroponically. Wheat has been produced at the rate of 60 g m -2 d -1, which could feed a human continuously from a farm only 13 m 2, but nearly continuous light equivalent to sunlight is required along with ideal temperatures, enriched CO 2, suitable cultivars, etc. Lower light results in more efficient photosynthesis but requires a larger farm, as do safety considerations and many crops.

  9. Antagonism between phytohormone signalling underlies the variation in disease susceptibility of tomato plants under elevated CO2.

    PubMed

    Zhang, Shuai; Li, Xin; Sun, Zenghui; Shao, Shujun; Hu, Lingfei; Ye, Meng; Zhou, Yanhong; Xia, Xiaojian; Yu, Jingquan; Shi, Kai

    2015-04-01

    Increasing CO2 concentrations ([CO2]) have the potential to disrupt plant-pathogen interactions in natural and agricultural ecosystems, but the research in this area has often produced conflicting results. Variations in phytohormone salicylic acid (SA) and jasmonic acid (JA) signalling could be associated with variations in the responses of pathogens to plants grown under elevated [CO2]. In this study, interactions between tomato plants and three pathogens with different infection strategies were compared. Elevated [CO2] generally favoured SA biosynthesis and signalling but repressed the JA pathway. The exposure of plants to elevated [CO2] revealed a lower incidence and severity of disease caused by tobacco mosaic virus (TMV) and by Pseudomonas syringae, whereas plant susceptibility to necrotrophic Botrytis cinerea increased. The elevated [CO2]-induced and basal resistance to TMV and P. syringae were completely abolished in plants in which the SA signalling pathway nonexpressor of pathogenesis-related genes 1 (NPR1) had been silenced or in transgenic plants defective in SA biosynthesis. In contrast, under both ambient and elevated [CO2], the susceptibility to B. cinerea highly increased in plants in which the JA signalling pathway proteinase inhibitors (PI) gene had been silenced or in a mutant affected in JA biosynthesis. However, plants affected in SA signalling remained less susceptible to this disease. These findings highlight the modulated antagonistic relationship between SA and JA that contributes to the variation in disease susceptibility under elevated [CO2]. This information will be critical for investigating how elevated CO2 may affect plant defence and the dynamics between plants and pathogens in both agricultural and natural ecosystems. © The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology.

  10. Insect stings to change gear for healthy plant: Improving maize drought tolerance by whitefly infestation.

    PubMed

    Park, Yong-Soon; Ryu, Choong-Min

    2016-05-03

    Since plants first appeared about 1.1 billion years ago, they have been faced with biotic and abiotic stresses in their environment. To overcome these stresses, plants developed defense strategies. Accumulating evidence suggests that the whitefly [Bemisia tabaci (Genn.)] affects the regulation of plant defenses and physiology. A recent study demonstrates that aboveground whitefly infestation positively modulates root biomass and anthocyanin pigmentation on brace roots of maize plants (Zea mays L.). In agreement with these observations, indole-3-acetic acid (IAA) and jasmonic acid (JA) contents and the expression of IAA- and JA-related genes are higher in whitefly-infested maize plants than in non-infected control plants. Interestingly, the fresh weight of whitefly-infested maize plants is approximately 20% higher than in non-infected control plants under water stress conditions. Further investigation has revealed that hydrogen peroxide (H2O2) accumulates in whitefly-infested maize plants after water stoppage. Taken together, these results suggest that activation of phytohormones- (i.e., IAA and JA) and H2O2-mediated maize signaling pathways triggered by aboveground whitefly infestation promotes drought resistance. They also provide an insight into how inter-kingdom interactions can improve drought tolerance in plants.

  11. Differences in soil quality between organic and conventional farming over a maize crop season

    NASA Astrophysics Data System (ADS)

    Ferreira, Carla; Veiga, Adelcia; Puga, João; Kikuchi, Ryunosuke; Ferreira, António

    2017-04-01

    Land degradation in agricultural areas is a major concern. The large number of mechanical interventions and the amount of inputs used to assure high crop productivity, such as fertilizers and pesticides, have negative impacts on soil quality and threaten crop productivity and environmental sustainability. Organic farming is an alternative agriculture system, based on organic fertilizers, biological pest control and crop rotation, in order to mitigate soil degradation. Maize is the third most important cereal worldwide, with 2008 million tons produced in 2013 (IGN, 2016). In Portugal, 120000 ha of arable land is devoted to maize production, leading to annual yields of about 930000 ton (INE, 2015). This study investigates soil quality differences in maize farms under organic and conventional systems. The study was carried out in Coimbra Agrarian Technical School (ESAC), in central region of Portugal. ESAC campus comprises maize fields managed under conventional farming - Vagem Grande (32 ha), and organic fields - Caldeirão (12 ha), distancing 2.8 km. Vagem Grande has been intensively used for grain maize production for more than 20 years, whereas Caldeirão was converted to organic farming in 2008, and is being used to select regional maize varieties. The region has a Mediterranean climate. The maize fields have Eutric Fluvisols, with gentle slopes (<3%). In order to assess soil quality, three plots per farm were installed in May 2006, immediately after sowing, and monitored until October 2016, before harvesting, in order to cover all the crop season. Each plot comprises 5 plant lines (˜4 m width) with 20 m length. In order to assure the comparison between both farms, the same maize variety was used (Pigarro) in both fields, with the same compass. Soil samples were collected immediately after sowing. In Vagem Grande distinct soil samples were taken: (i) within plant lines, and (ii) between plant lines, since mineral fertilizers were spread over the field before

  12. Causes and effects of poor drainage in cranberry farms

    USDA-ARS?s Scientific Manuscript database

    Soils that support the growth of cranberries often cap geologic deposits of organic sediment, such as peat. However, low-permeability organic sediments may result in inadequate drainage that diminishes plant productivity and enhances fruit rot. Consequently, new constructions of cranberry farms are ...

  13. Ten Years of Plant Pathology Research at the Cook Agronomy Farm: What Have We Learned?

    USDA-ARS?s Scientific Manuscript database

    The Cook Agronomy Farm has provided important information for understanding root diseases under directseeded conditions in the higher rainfall annual cropping zones of the Palouse, at a landscape scale. This farm has served as an important outdoor laboratory to test disease management techniques suc...

  14. Farm Safety Practices and Farm Size in New South Wales.

    PubMed

    Bailey, Jannine; Dutton, Tegan; Payne, Kristy; Wilson, Ross; Brew, Bronwyn K

    2017-01-01

    There is some evidence to suggest that safety on small-area farms may not be high priority due to economic constraints and lack of knowledge. This has important ramifications for injury and economic burden. The objective of this research was to conduct a pilot study to investigate whether small- to medium-area farms implement fewer safety practices than large-area farms. Farmers were recruited from farm safety training days, field days, and produce stores in rural New South Wales (NSW), Australia. Small- and medium-area farms less than 500 ha (1235 acres) in size were aggregated for analysis and compared with large-area farms (≥500 ha) for survey items, including safety equipment owned and used, safety practices protecting children, barriers to improving safety, and causes of injury. Overall, small/medium-area farms were found to own less safety equipment and to employ less safety practices than large-area farms. In particular, fewer tractors were fitted with rollover protection structures, there was less signage, less hearing protection, and fewer machinery guides. Injury rates were slightly less for small/medium-area farms, particularly involving vehicles. Small- and medium-area farmers were more likely to report lack of skills as barriers to making safety improvements. This pilot study found some evidence that small/medium-area farms implement fewer safety practices than large-area farms. A larger study is warranted to investigate this further, with particular focus on barriers and ways to overcome them. This could have important ramifications for government policies supporting struggling farmers on small/medium-area farms.

  15. Study on the extraction, purification and quantification of jasmonic acid, abscisic acid and indole-3-acetic acid in plants.

    PubMed

    Zhang, Feng Juan; Jin, You Ju; Xu, Xing You; Lu, Rong Chun; Chen, Hua Jun

    2008-01-01

    Jasmonic acid (JA), abscisic acid (ABA) and indole-3-acetic acid (IAA) are important plant hormones. Plant hormones are difficult to analyse because they occur in small concentrations and other substances in the plant interfere with their detection. To develop a new, inexpensive procedure for the rapid extraction and purification of IAA, ABA and JA from various plant species. Samples were prepared by extraction of plant tissues with methanol and ethyl acetate. Then the extracts were further purified and enriched with C(18) cartridges. The final extracts were derivatised with diazomethane and then measured by GC-MS. The results of the new methodology were compared with those of the Creelman and Mullet procedure. Sequential elution of the assimilates from the C(18 )cartridges revealed that IAA and ABA eluted in 40% methanol, while JA subsequently eluted in 60% methanol. The new plant hormone extraction and purification procedure produced results that were comparable to those obtained with the Creelman and Mullet's procedure. This new procedure requires only 0.5 g leaf samples to quantify these compounds with high reliability and can simultaneously determine the concentrations of the three plant hormones. A simple, inexpensive method was developed for determining endogenous IAA, ABA and JA concentrations in plant tissue.

  16. Plant photomorphogenesis and canopy growth

    NASA Technical Reports Server (NTRS)

    Ballare, Carlos L.; Scopel, Ana L.

    1994-01-01

    An important motivation for studying photomorphogenesis is to understand the relationships among plant photophysiology in canopies, canopy productivity, and agronomic yield. This understanding is essential to optimize lighting systems used for plant farming in controlled environments (CE) and for the design of genetically engineered crop strains with altered photoresponses. This article provides an overview of some basic principles of plant photomorphogenesis in canopies and discusses their implications for (1) scaling up information on plant photophysiology from individual plants in CE to whole canopies in the field, and (2) designing lighting conditions to increase plant productivity in CE used for agronomic purposes (e.g. space farming in CE Life Support Systems). We concentrate on the visible (lambda between 400 and 700 nm) and far-infrared (FR; lambda greater than 700 nm) spectral regions, since the ultraviolet (UV; 280 to 400 nm) is covered by other authors in this volume.

  17. Plant photomorphogenesis and canopy growth

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Ballare, C.L.; Scopel, A.L.

    1994-12-31

    An important motivation for studying photomorphogenesis is to understand the relationships among plant photophysiology in canopies, canopy productivity, and agronomic yield. This understanding is essential to optimize lighting systems used for plant farming in controlled environments (CE) and for the design of genetically engineered crop strains with altered photoresponses. This article provides an overview of some basic principles of plant photomorphogenesis in canopies and discusses their implications for (1) scaling up information on plant photophysiology from individual plants in CE to whole canopies in the field, and (2), designing lighting conditions to increase plant productivity in CE used for agronomicmore » purposes [e.g. space farming in CE Life-Support-Systems]. We concentrate on the visible ({lambda} between 400 and 700 nm) and far red (FR; {lambda} > 700 nm) spectral regions, since the ultraviolet (UV; 280 to 400 nm) is covered by other authors in this volume.« less

  18. The assembly of ant-farmed gardens: mutualism specialization following host broadening

    PubMed Central

    Janda, Milan

    2017-01-01

    Ant-gardens (AGs) are ant/plant mutualisms in which ants farm epiphytes in return for nest space and food rewards. They occur in the Neotropics and Australasia, but not in Africa, and their evolutionary assembly remains unclear. We here use phylogenetic frameworks for important AG lineages in Australasia, namely the ant genus Philidris and domatium-bearing ferns (Lecanopteris) and flowering plants in the Apocynaceae (Hoya and Dischidia) and Rubiaceae (Myrmecodia, Hydnophytum, Anthorrhiza, Myrmephytum and Squamellaria). Our analyses revealed that in these clades, diaspore dispersal by ants evolved at least 13 times, five times in the Late Miocene and Pliocene in Australasia and seven times during the Pliocene in Southeast Asia, after Philidris ants had arrived there, with subsequent dispersal between these two areas. A uniquely specialized AG system evolved in Fiji at the onset of the Quaternary. The farming in the same AG of epiphytes that do not offer nest spaces suggests that a broadening of the ants' plant host spectrum drove the evolution of additional domatium-bearing AG-epiphytes by selecting on pre-adapted morphological traits. Consistent with this, we found a statistical correlation between the evolution of diaspore dispersal by ants and domatia in all three lineages. Our study highlights how host broadening by a symbiont has led to new farming mutualisms. PMID:28298344

  19. Did farming arise from a misapplication of social intelligence?

    PubMed

    Mithen, Steven

    2007-04-29

    The origins of farming is the defining event of human history--the one turning point that has resulted in modern humans having a quite different type of lifestyle and cognition to all other animals and past types of humans. With the economic basis provided by farming, human individuals and societies have developed types of material culture that greatly augment powers of memory and computation, extending the human mental capacity far beyond that which the brain alone can provide. Archaeologists have long debated and discussed why people began living in settled communities and became dependent on cultivated plants and animals, which soon evolved into domesticated forms. One of the most intriguing explanations was proposed more than 20 years ago not by an archaeologist but by a psychologist: Nicholas Humphrey suggested that farming arose from the 'misapplication of social intelligence'. I explore this idea in relation to recent discoveries and archaeological interpretations in the Near East, arguing that social intelligence has indeed played a key role in the origin of farming and hence the emergence of the modern world.

  20. Generic Escherichia coli Contamination of Spinach at the Preharvest Stage: Effects of Farm Management and Environmental Factors

    PubMed Central

    Navratil, Sarah; Gregory, Ashley; Bauer, Arin; Srinath, Indumathi; Jun, Mikyoung; Szonyi, Barbara; Nightingale, Kendra; Anciso, Juan; Ivanek, Renata

    2013-01-01

    The objective of this study was to determine the effects of farm management and environmental factors on preharvest spinach contamination with generic Escherichia coli as an indicator of fecal contamination. A repeated cross-sectional study was conducted by visiting spinach farms up to four times per growing season over a period of 2 years (2010 to 2011). Spinach samples (n = 955) were collected from 12 spinach farms in Colorado and Texas as representative states of the Western and Southwestern United States, respectively. During each farm visit, farmers were surveyed about farm-related management and environmental factors using a questionnaire. Associations between the prevalence of generic E. coli in spinach and farm-related factors were assessed by using a multivariable logistic regression model including random effects for farm and farm visit. Overall, 6.6% of spinach samples were positive for generic E. coli. Significant risk factors for spinach contamination with generic E. coli were the proximity (within 10 miles) of a poultry farm, the use of pond water for irrigation, a >66-day period since the planting of spinach, farming on fields previously used for grazing, the production of hay before spinach planting, and the farm location in the Southwestern United States. Contamination with generic E. coli was significantly reduced with an irrigation lapse time of >5 days as well as by several factors related to field workers, including the use of portable toilets, training to use portable toilets, and the use of hand-washing stations. To our knowledge, this is the first report of an association between field workers' personal hygiene and produce contamination with generic E. coli at the preharvest level. Collectively, our findings support that practice of good personal hygiene and other good farm management practices may reduce produce contamination with generic E. coli at the preharvest level. PMID:23666336

  1. Farm-level economics of innovative tillage technologies: the case of no-till in the Altai Krai in Russian Siberia.

    PubMed

    Bavorova, Miroslava; Imamverdiyev, Nizami; Ponkina, Elena

    2018-01-01

    In the agricultural Altai Krai in Russian Siberia, soil degradation problems are prevalent. Agronomists recommend "reduced tillage systems," especially no-till, as a sustainable way to cultivate land that is threatened by soil degradation. In the Altai Krai, less is known about the technologies in practice. In this paper, we provide information on plant cultivation technologies used in the Altai Krai and on selected factors preventing farm managers in this region from adopting no-till technology based on our own quantitative survey conducted across 107 farms in 2015 and 2016. The results of the quantitative survey show that farm managers have high uncertainty regarding the use of no-till technology including its economics. To close this gap, we provide systematic analysis of factors influencing the economy of the plant production systems by using a farm optimization model (linear programming) for a real farm, together with expert estimations. The farm-specific results of the optimization model show that under optimal management and climatic conditions, the expert Modern Canadian no-till technology outperforms the farm min-till technology, but this is not the case for suboptimal conditions with lower yields.

  2. Farm accidents and injuries among farm families and workers. A pilot study.

    PubMed

    Cummings, P H

    1991-09-01

    Farm accident facts traditionally have been difficult to collect because of the wide array of farm family and non-family involvement in farming practices. Areas commonly involved in farm related accidents include farm machinery, tractor overturns, farm animals, farm trucks, hand and power tools, household items, chemicals, and garden equipment. Two purposes of this descriptive study were to examine, over a 1 year period, the demographic features and types, severity, and mechanisms of injury among farm families and their workers in a representative county in South Carolina, and to develop a two part mail-out questionnaire for data collection relative to farm work related accidents. The researcher concluded that farm accidents are sparsely researched; that traditional data collection methods are difficult, expensive, and time consuming; and that mail-out questionnaires are not a very effective method of collecting data relative to farm accidents, since farmers proved very reluctant to report accidents.

  3. How to induce defense responses in wild plant populations? Using bilberry (Vaccinium myrtillus) as example.

    PubMed

    Seldal, Tarald; Hegland, Stein Joar; Rydgren, Knut; Rodriguez-Saona, Cesar; Töpper, Joachim Paul

    2017-03-01

    Inducible plant defense is a beneficial strategy for plants, which imply that plants should allocate resources from growth and reproduction to defense when herbivores attack. Plant ecologist has often studied defense responses in wild populations by biomass clipping experiments, whereas laboratory and greenhouse experiments in addition apply chemical elicitors to induce defense responses. To investigate whether field ecologists could benefit from methods used in laboratory and greenhouse studies, we established a randomized block-design in a pine-bilberry forest in Western Norway. We tested whether we could activate defense responses in bilberry ( Vaccinium myrtillus ) by nine different treatments using clipping (leaf tissue or branch removal) with or without chemical treatment by methyljasmonate (MeJA). We subsequently measured consequences of induced defenses through vegetative growth and insect herbivory during one growing season. Our results showed that only MeJA-treated plants showed consistent defense responses through suppressed vegetative growth and reduced herbivory by leaf-chewing insects, suggesting an allocation of resources from growth to defense. Leaf tissue removal reduced insect herbivory equal to the effect of the MeJa treatments, but had no negative impact on growth. Branch removal did not reduce insect herbivory or vegetative growth. MeJa treatment and clipping combined did not give an additional defense response. In this study, we investigated how to induce defense responses in wild plant populations under natural field conditions. Our results show that using the chemical elicitor MeJA, with or without biomass clipping, may be a better method to induce defense response in field experiments than clipping of leaves or branches that often has been used in ecological field studies.

  4. Exogenous application of methyl jasmonate induces a defense response and resistance against Sclerotinia sclerotiorum in dry bean plants.

    PubMed

    Oliveira, Marília Barros; Junior, Murillo Lobo; Grossi-de-Sá, Maria Fátima; Petrofeza, Silvana

    2015-06-15

    Sclerotinia sclerotiorum (Lib.) de Bary is a necrotrophic fungal pathogen that causes a disease known as white mold, which is a major problem for dry bean (Phaseolus vulgaris L.) and other crops in many growing areas in Brazil. To investigate the role of methyl jasmonate (MeJA) in defending dry bean plants against S. sclerotiorum, we used suppression subtractive hybridization (SSH) of cDNA and identified genes that are differentially expressed during plant-pathogen interactions after treatment. Exogenous MeJA application enhanced resistance to the pathogen, and SSH analyses led to the identification of 94 unigenes, presumably involved in a variety of functions, which were classified into several functional categories, including metabolism, signal transduction, protein biogenesis and degradation, and cell defense and rescue. Using RT-qPCR, some unigenes were found to be differentially expressed in a time-dependent manner in dry bean plants during the interaction with S. sclerotiorum after MeJA treatment, including the pathogenesis-related protein PR3 (chitinase), PvCallose (callose synthase), PvNBS-LRR (NBS-LRR resistance-like protein), PvF-box (F-box family protein-like), and a polygalacturonase inhibitor protein (PGIP). Based on these expression data, the putative roles of differentially expressed genes were discussed in relation to the disease and MeJA resistance induction. Changes in the activity of the pathogenesis-related proteins β-1,3-glucanase, chitinase, phenylalanine ammonia-lyase, and peroxidase in plants after MeJA treatment and following inoculation of the pathogen were also investigated as molecular markers of induced resistance. Foliar application of MeJA induced partial resistance against S. sclerotiorum in plants as well as a consistent increase in pathogenesis-related protein activities. Our findings provide new insights into the physiological and molecular mechanisms of resistance induced by MeJA in the P. vulgaris-S. sclerotiorum pathosystem

  5. Clostridium botulinum in Scottish fish farms and farmed trout.

    PubMed

    Burns, G F; Williams, H

    1975-02-01

    Rainbow trout and specimens of pond mud were collected from three fish farms and examined for the presence of Clostridium botulinum. Two of the farms were constructed with concrete channels and one was mud-bottomed. Cl. botulinum was isolated only from the mud-bottomed farm (24% of muds), and the isolates were all non-proteolytic type B. The implications of the presence of Cl. botulinum spores in the mud of fish farms is discussed.

  6. Transcriptome sequencing and de novo analysis of cytoplasmic male sterility and maintenance in JA-CMS cotton.

    PubMed

    Yang, Peng; Han, Jinfeng; Huang, Jinling

    2014-01-01

    Cytoplasmic male sterility (CMS) is the failure to produce functional pollen, which is inherited maternally. And it is known that anther development is modulated through complicated interactions between nuclear and mitochondrial genes in sporophytic and gametophytic tissues. However, an unbiased transcriptome sequencing analysis of CMS in cotton is currently lacking in the literature. This study compared differentially expressed (DE) genes of floral buds at the sporogenous cells stage (SS) and microsporocyte stage (MS) (the two most important stages for pollen abortion in JA-CMS) between JA-CMS and its fertile maintainer line JB cotton plants, using the Illumina HiSeq 2000 sequencing platform. A total of 709 (1.8%) DE genes including 293 up-regulated and 416 down-regulated genes were identified in JA-CMS line comparing with its maintainer line at the SS stage, and 644 (1.6%) DE genes with 263 up-regulated and 381 down-regulated genes were detected at the MS stage. By comparing the two stages in the same material, there were 8 up-regulated and 9 down-regulated DE genes in JA-CMS line and 29 up-regulated and 9 down-regulated DE genes in JB maintainer line at the MS stage. Quantitative RT-PCR was used to validate 7 randomly selected DE genes. Bioinformatics analysis revealed that genes involved in reduction-oxidation reactions and alpha-linolenic acid metabolism were down-regulated, while genes pertaining to photosynthesis and flavonoid biosynthesis were up-regulated in JA-CMS floral buds compared with their JB counterparts at the SS and/or MS stages. All these four biological processes play important roles in reactive oxygen species (ROS) homeostasis, which may be an important factor contributing to the sterile trait of JA-CMS. Further experiments are warranted to elucidate molecular mechanisms of these genes that lead to CMS.

  7. Transcriptome Sequencing and De Novo Analysis of Cytoplasmic Male Sterility and Maintenance in JA-CMS Cotton

    PubMed Central

    Yang, Peng; Han, Jinfeng; Huang, Jinling

    2014-01-01

    Cytoplasmic male sterility (CMS) is the failure to produce functional pollen, which is inherited maternally. And it is known that anther development is modulated through complicated interactions between nuclear and mitochondrial genes in sporophytic and gametophytic tissues. However, an unbiased transcriptome sequencing analysis of CMS in cotton is currently lacking in the literature. This study compared differentially expressed (DE) genes of floral buds at the sporogenous cells stage (SS) and microsporocyte stage (MS) (the two most important stages for pollen abortion in JA-CMS) between JA-CMS and its fertile maintainer line JB cotton plants, using the Illumina HiSeq 2000 sequencing platform. A total of 709 (1.8%) DE genes including 293 up-regulated and 416 down-regulated genes were identified in JA-CMS line comparing with its maintainer line at the SS stage, and 644 (1.6%) DE genes with 263 up-regulated and 381 down-regulated genes were detected at the MS stage. By comparing the two stages in the same material, there were 8 up-regulated and 9 down-regulated DE genes in JA-CMS line and 29 up-regulated and 9 down-regulated DE genes in JB maintainer line at the MS stage. Quantitative RT-PCR was used to validate 7 randomly selected DE genes. Bioinformatics analysis revealed that genes involved in reduction-oxidation reactions and alpha-linolenic acid metabolism were down-regulated, while genes pertaining to photosynthesis and flavonoid biosynthesis were up-regulated in JA-CMS floral buds compared with their JB counterparts at the SS and/or MS stages. All these four biological processes play important roles in reactive oxygen species (ROS) homeostasis, which may be an important factor contributing to the sterile trait of JA-CMS. Further experiments are warranted to elucidate molecular mechanisms of these genes that lead to CMS. PMID:25372034

  8. Partial Activation of SA- and JA-Defensive Pathways in Strawberry upon Colletotrichum acutatum Interaction.

    PubMed

    Amil-Ruiz, Francisco; Garrido-Gala, José; Gadea, José; Blanco-Portales, Rosario; Muñoz-Mérida, Antonio; Trelles, Oswaldo; de Los Santos, Berta; Arroyo, Francisco T; Aguado-Puig, Ana; Romero, Fernando; Mercado, José-Ángel; Pliego-Alfaro, Fernando; Muñoz-Blanco, Juan; Caballero, José L

    2016-01-01

    Understanding the nature of pathogen host interaction may help improve strawberry (Fragaria × ananassa) cultivars. Plant resistance to pathogenic agents usually operates through a complex network of defense mechanisms mediated by a diverse array of signaling molecules. In strawberry, resistance to a variety of pathogens has been reported to be mostly polygenic and quantitatively inherited, making it difficult to associate molecular markers with disease resistance genes. Colletotrichum acutatum spp. is a major strawberry pathogen, and completely resistant cultivars have not been reported. Moreover, strawberry defense network components and mechanisms remain largely unknown and poorly understood. Assessment of the strawberry response to C. acutatum included a global transcript analysis, and acidic hormones SA and JA measurements were analyzed after challenge with the pathogen. Induction of transcripts corresponding to the SA and JA signaling pathways and key genes controlling major steps within these defense pathways was detected. Accordingly, SA and JA accumulated in strawberry after infection. Contrastingly, induction of several important SA, JA, and oxidative stress-responsive defense genes, including FaPR1-1, FaLOX2, FaJAR1, FaPDF1, and FaGST1, was not detected, which suggests that specific branches in these defense pathways (those leading to FaPR1-2, FaPR2-1, FaPR2-2, FaAOS, FaPR5, and FaPR10) were activated. Our results reveal that specific aspects in SA and JA dependent signaling pathways are activated in strawberry upon interaction with C. acutatum. Certain described defense-associated transcripts related to these two known signaling pathways do not increase in abundance following infection. This finding suggests new insight into a specific putative molecular strategy for defense against this pathogen.

  9. Partial Activation of SA- and JA-Defensive Pathways in Strawberry upon Colletotrichum acutatum Interaction

    PubMed Central

    Amil-Ruiz, Francisco; Garrido-Gala, José; Gadea, José; Blanco-Portales, Rosario; Muñoz-Mérida, Antonio; Trelles, Oswaldo; de los Santos, Berta; Arroyo, Francisco T.; Aguado-Puig, Ana; Romero, Fernando; Mercado, José-Ángel; Pliego-Alfaro, Fernando; Muñoz-Blanco, Juan; Caballero, José L.

    2016-01-01

    Understanding the nature of pathogen host interaction may help improve strawberry (Fragaria × ananassa) cultivars. Plant resistance to pathogenic agents usually operates through a complex network of defense mechanisms mediated by a diverse array of signaling molecules. In strawberry, resistance to a variety of pathogens has been reported to be mostly polygenic and quantitatively inherited, making it difficult to associate molecular markers with disease resistance genes. Colletotrichum acutatum spp. is a major strawberry pathogen, and completely resistant cultivars have not been reported. Moreover, strawberry defense network components and mechanisms remain largely unknown and poorly understood. Assessment of the strawberry response to C. acutatum included a global transcript analysis, and acidic hormones SA and JA measurements were analyzed after challenge with the pathogen. Induction of transcripts corresponding to the SA and JA signaling pathways and key genes controlling major steps within these defense pathways was detected. Accordingly, SA and JA accumulated in strawberry after infection. Contrastingly, induction of several important SA, JA, and oxidative stress-responsive defense genes, including FaPR1-1, FaLOX2, FaJAR1, FaPDF1, and FaGST1, was not detected, which suggests that specific branches in these defense pathways (those leading to FaPR1-2, FaPR2-1, FaPR2-2, FaAOS, FaPR5, and FaPR10) were activated. Our results reveal that specific aspects in SA and JA dependent signaling pathways are activated in strawberry upon interaction with C. acutatum. Certain described defense-associated transcripts related to these two known signaling pathways do not increase in abundance following infection. This finding suggests new insight into a specific putative molecular strategy for defense against this pathogen. PMID:27471515

  10. Agromining: farming for metals in the future?

    PubMed

    van der Ent, Antony; Baker, Alan J M; Reeves, Roger D; Chaney, Rufus L; Anderson, Christopher W N; Meech, John A; Erskine, Peter D; Simonnot, Marie-Odile; Vaughan, James; Morel, Jean Louis; Echevarria, Guillaume; Fogliani, Bruno; Rongliang, Qiu; Mulligan, David R

    2015-04-21

    Phytomining technology employs hyperaccumulator plants to take up metal in harvestable plant biomass. Harvesting, drying and incineration of the biomass generates a high-grade bio-ore. We propose that "agromining" (a variant of phytomining) could provide local communities with an alternative type of agriculture on degraded lands; farming not for food crops, but for metals such as nickel (Ni). However, two decades after its inception and numerous successful experiments, commercial phytomining has not yet become a reality. To build the case for the minerals industry, a large-scale demonstration is needed to identify operational risks and provide "real-life" evidence for profitability.

  11. Fiscalini Farms Biomass Energy Project

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    William Stringfellow; Mary Kay Camarillo; Jeremy Hanlon

    2011-09-30

    In this final report describes and documents research that was conducted by the Ecological Engineering Research Program (EERP) at the University of the Pacific (Stockton, CA) under subcontract to Fiscalini Farms LP for work under the Assistance Agreement DE-EE0001895 'Measurement and Evaluation of a Dairy Anaerobic Digestion/Power Generation System' from the United States Department of Energy, National Energy Technology Laboratory. Fiscalini Farms is operating a 710 kW biomass-energy power plant that uses bio-methane, generated from plant biomass, cheese whey, and cattle manure via mesophilic anaerobic digestion, to produce electricity using an internal combustion engine. The primary objectives of the projectmore » were to document baseline conditions for the anaerobic digester and the combined heat and power (CHP) system used for the dairy-based biomass-energy production. The baseline condition of the plant was evaluated in the context of regulatory and economic constraints. In this final report, the operation of the plant between start-up in 2009 and operation in 2010 are documented and an interpretation of the technical data is provided. An economic analysis of the biomass energy system was previously completed (Appendix A) and the results from that study are discussed briefly in this report. Results from the start-up and first year of operation indicate that mesophilic anaerobic digestion of agricultural biomass, combined with an internal combustion engine, is a reliable source of alternative electrical production. A major advantage of biomass energy facilities located on dairy farms appears to be their inherent stability and ability to produce a consistent, 24 hour supply of electricity. However, technical analysis indicated that the Fiscalini Farms system was operating below capacity and that economic sustainability would be improved by increasing loading of feedstocks to the digester. Additional operational modifications, such as increased utilization of

  12. Plant disease management in organic farming systems.

    PubMed

    van Bruggen, Ariena H C; Gamliel, Abraham; Finckh, Maria R

    2016-01-01

    Organic farming (OF) has significantly increased in importance in recent decades. Disease management in OF is largely based on the maintenance of biological diversity and soil health by balanced crop rotations, including nitrogen-fixing and cover crops, intercrops, additions of manure and compost and reductions in soil tillage. Most soil-borne diseases are naturally suppressed, while foliar diseases can sometimes be problematic. Only when a severe disease outbreak is expected are pesticides used that are approved for OF. A detailed overview is given of cultural and biological control measures. Attention is also given to regulated pesticides. We conclude that a systems approach to disease management is required, and that interdisciplinary research is needed to solve lingering disease problems, especially for OF in the tropics. Some of the organic regulations are in need of revision in close collaboration with various stakeholders. © 2015 Society of Chemical Industry.

  13. Green and profitable farming systems for the southeastern USA

    USDA-ARS?s Scientific Manuscript database

    Although most of the farms of the southeastern USA are relatively small, they often have profit centers of livestock or high value crops. Thus, agribusiness (livestock, timber, and high value crops) is critical to the region’s economy. The region has temperatures that allow plant growth for the enti...

  14. On-farm production of arbuscular mycorrhizal funus inoculum in compost and vermiculite mixtures: results of on-farm demonstrations and impact of compost microbiological quality

    USDA-ARS?s Scientific Manuscript database

    The sustainability and profitability of many agricultural systems can be enhanced through the utilization of inoculum of arbuscular mycorrhizal fungi. Inocula are commercially available, but inoculum can also be produced on-farm in mixtures of compost and vermiculite with a nurse host plant. Demon...

  15. Effects of fish farm loadings on seagrass (Posidonia oceanica) distribution, growth and photosynthesis.

    PubMed

    Ruiz, J M; Pérez, M; Romero, J

    2001-09-01

    The spatial extent and timing of the impact of fish farms on the distribution and performance of a Posidonia oceanica meadow were examined in an embayment of the south-eastern coast of Spain (Hornillo Bay, Murcia). Changes in seagrass distribution were determined using available seagrass mapping (from 1988, i.e., before the onset of aquaculture activities and 1998) and by successive sampling in 1994 and 1998. Environmental variables (light attenuation coefficient, water-column dissolved nutrients and organic content of sediments) together with plant performance (shoot biomass, leaf growth rate, photosynthetic activity, carbohydrate reserves, the number of leaves per shoot, epiphyte loads and herbivore pressure) were measured in plants affected by organic discharges, and were compared with those found in reference healthy plants over an annual growth cycle. Since the onset of fish farm activity, 11.29 ha of P. oceanica meadow has been completely lost and 9.86 ha significantly degraded, thus resulting in a total affected area which accounts for about 53% of the former meadow, or 7-fold the fish farming area. Unequal propagation of seagrass die-off or degradation reflects the relevance of local factors such as depth and hydrodynamism on the true extent of fish farm impact. Water transparency decreases and dissolved nutrient and organic content of sediments increases in the vicinity of cages compared to distant reference stations, thus supporting the notion of environmental gradients caused by the organic release from cages, which spreads outwards. Shoot size, leaf growth rate and the number of leaves per shoot in plants close to the fish farm decreased. Moreover, low leaf growth and low rhizome carbohydrate concentration (always relative to that found in an undisturbed area) indicated carbon budget imbalances. Since light reduction in the affected area was only modest (31% of light reaching the sea surface, while at the same depth this figure was 39% at the reference

  16. 29 CFR 780.143 - Practices on a farm not performed for the farmer.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... communications lines is part of a nonfarming enterprise outside the scope of agriculture. When a packer of... employees, and transports the harvested crop to his off-the-farm packing or dehydrating plant, the transporting and plant employees, who are not engaged in “primary” agriculture as are the harvesting employees...

  17. Whole-farm phosphorus loss from grazing-based dairy farms

    USDA-ARS?s Scientific Manuscript database

    Phosphorus (P) loss from agricultural farms persists as a water quality impairment issue. For dairy farms, P can be lost from cropland, pastures, and open-air lots. We used interview surveys to document land use, cattle herd characteristics, and manure management for four grazing-based dairy farms i...

  18. Employment Decisions of Farm Couples: Full-Time or Part-Time Farming?

    ERIC Educational Resources Information Center

    Wozniak, Patricia J.; Scholl, Kathleen K.

    1988-01-01

    The employment of one farm spouse is positively related to the off-farm employment of the other spouse. Wives' off-farm employment is most closely related to their personal characteristics, whereas husbands' decisions are best predicted by a combination of farm, family, and personal characteristics. (Author)

  19. Involvement of Wives in Farm Tasks as Related to Characteristics of the Farm, the Family and Work Off the Farm.

    ERIC Educational Resources Information Center

    Wilkening, Eugene A.; Ahrens, Nancy

    In an attempt to determine the basis of the farm wife's involvement in farm tasks, hypotheses regarding farm size and type, family cycle and wife's age, off-farm work of husband or wife, and family educational levels were tested in a 1978 random questionnaire survey of 532 Wisconsin farm families. As expected, wives were more involved with farm…

  20. Linking agricultural practices, mycorrhizal fungi, and traits mediating plant-insect interactions.

    PubMed

    Barber, Nicholas A; Kiers, E Toby; Theis, Nina; Hazzard, Ruth V; Adler, Lynn S

    2013-10-01

    Agricultural management has profound effects on soil communities. Activities such as fertilizer inputs can modify the composition of arbuscular mycorrhizal fungi (AMF) communities, which form important symbioses with the roots of most crop plants. Intensive conventional agricultural management may select for less mutualistic AMF with reduced benefits to host plants compared to organic management, but these differences are poorly understood. AMF are generally evaluated based on their direct growth effects on plants. However, mycorrhizal colonization also may alter plant traits such as tissue nutrients, defensive chemistry, or floral traits, which mediate important plant-insect interactions like herbivory and pollination. To determine the effect of AMF from different farming practices on plant performance and traits that putatively mediate species interactions, we performed a greenhouse study by inoculating Cucumis sativus (cucumber, Cucurbitaceae) with AMF from conventional farms, organic farms, and a commercial AMF inoculum. We measured growth and a suite of plant traits hypothesized to be important predictors of herbivore resistance and pollinator attraction. Several leaf and root traits and flower production were significantly affected by AMF inoculum. Both conventional and organic AMF reduced leaf P content but increased Na content compared to control and commercial AMF. Leaf defenses were unaffected by AMF treatments, but conventional AMF increased root cucurbitacin C, the primary defensive chemical of C. sativus, compared to organic AMF. These effects may have important consequences for herbivore preference and population dynamics. AMF from both organic and conventional farms decreased flower production relative to commercial and control treatments, which may reduce pollinator attraction and plant reproduction. AMF from both farm types also reduced seed germination, but effects on plant growth were limited. Our results suggest that studies only considering AMF

  1. Neolithic dairy farming at the extreme of agriculture in northern Europe

    PubMed Central

    Cramp, Lucy J. E.; Evershed, Richard P.; Lavento, Mika; Halinen, Petri; Mannermaa, Kristiina; Oinonen, Markku; Kettunen, Johannes; Perola, Markus; Onkamo, Päivi; Heyd, Volker

    2014-01-01

    The conventional ‘Neolithic package’ comprised animals and plants originally domesticated in the Near East. As farming spread on a generally northwest trajectory across Europe, early pastoralists would have been faced with the challenge of making farming viable in regions in which the organisms were poorly adapted to providing optimal yields or even surviving. Hence, it has long been debated whether Neolithic economies were ever established at the modern limits of agriculture. Here, we examine food residues in pottery, testing a hypothesis that Neolithic farming was practiced beyond the 60th parallel north. Our findings, based on diagnostic biomarker lipids and δ13C values of preserved fatty acids, reveal a transition at ca 2500 BC from the exploitation of aquatic organisms to processing of ruminant products, specifically milk, confirming farming was practiced at high latitudes. Combining this with genetic, environmental and archaeological information, we demonstrate the origins of dairying probably accompanied an incoming, genetically distinct, population successfully establishing this new subsistence ‘package’. PMID:25080345

  2. Interactions between the jasmonic and salicylic acid pathway modulate the plant metabolome and affect herbivores of different feeding types.

    PubMed

    Schweiger, R; Heise, A-M; Persicke, M; Müller, C

    2014-07-01

    The phytohormones jasmonic acid (JA) and salicylic acid (SA) mediate induced plant defences and the corresponding pathways interact in a complex manner as has been shown on the transcript and proteine level. Downstream, metabolic changes are important for plant-herbivore interactions. This study investigated metabolic changes in leaf tissue and phloem exudates of Plantago lanceolata after single and combined JA and SA applications as well as consequences on chewing-biting (Heliothis virescens) and piercing-sucking (Myzus persicae) herbivores. Targeted metabolite profiling and untargeted metabolic fingerprinting uncovered different categories of plant metabolites, which were influenced in a specific manner, indicating points of divergence, convergence, positive crosstalk and pronounced mutual antagonism between the signaling pathways. Phytohormone-specific decreases of primary metabolite pool sizes in the phloem exudates may indicate shifts in sink-source relations, resource allocation, nutrient uptake or photosynthesis. Survival of both herbivore species was significantly reduced by JA and SA treatments. However, the combined application of JA and SA attenuated the negative effects at least against H. virescens suggesting that mutual antagonism between the JA and SA pathway may be responsible. Pathway interactions provide a great regulatory potential for the plant that allows triggering of appropriate defences when attacked by different antagonist species. © 2013 John Wiley & Sons Ltd.

  3. A broad-spectrum, efficient and nontransgenic approach to control plant viruses by application of salicylic acid and jasmonic acid.

    PubMed

    Shang, Jing; Xi, De-Hui; Xu, Fei; Wang, Shao-Dong; Cao, Sen; Xu, Mo-Yun; Zhao, Ping-Ping; Wang, Jian-Hui; Jia, Shu-Dan; Zhang, Zhong-Wei; Yuan, Shu; Lin, Hong-Hui

    2011-02-01

    Plant viruses cause many diseases that lead to significant economic losses. However, most of the approaches to control plant viruses, including transgenic processes or drugs are plant-species-limited or virus-species-limited, and not very effective. We introduce an application of jasmonic acid (JA) and salicylic acid (SA), a broad-spectrum, efficient and nontransgenic method, to improve plant resistance to RNA viruses. Applying 0.06 mM JA and then 0.1 mM SA 24 h later, enhanced resistance to Cucumber mosaic virus (CMV), Tobacco mosaic virus (TMV) and Turnip crinkle virus (TCV) in Arabidopsis, tobacco, tomato and hot pepper. The inhibition efficiency to virus replication usually achieved up to 80-90%. The putative molecular mechanism was investigated. Some possible factors affecting the synergism of JA and SA have been defined, including WRKY53, WRKY70, PDF1.2, MPK4, MPK2, MPK3, MPK5, MPK12, MPK14, MKK1, MKK2, and MKK6. All genes involving in the synergism of JA and SA were investigated. This approach is safe to human beings and environmentally friendly and shows potential as a strong tool for crop protection against plant viruses.

  4. The Interactive Effect of Diversification and Farming Scale on Productivity of Family Farm:Taking Rice Cultivation as An Example

    NASA Astrophysics Data System (ADS)

    Wei, Zhou

    2017-05-01

    Based on the diversification and cultivation scale, the rice cropping data of rural fixed observation points in 2011 were selected and the effect of diversification degree on rice productivity was analyzed by the Tobit model. The empirical results of the model show that diversification of sample farm will lead to loss of rice production efficiency. With the increase of rice planting scale, the loss of rice production efficiency will need to be further increased by diversification. Thus, we should stick to the family farm of specialized production operation. The transfer of land, the price and quantity of leasing, respecting the law of the market; the raising of funds can be considered non-subsidized capital market financing to help, while maintaining a certain degree of diversification, to avoid idle assets, low resource efficiency loss.

  5. Arachidonic Acid: An Evolutionarily Conserved Signaling Molecule Modulates Plant Stress Signaling Networks[C][W

    PubMed Central

    Savchenko, Tatyana; Walley, Justin W.; Chehab, E. Wassim; Xiao, Yanmei; Kaspi, Roy; Pye, Matthew F.; Mohamed, Maged E.; Lazarus, Colin M.; Bostock, Richard M.; Dehesh, Katayoon

    2010-01-01

    Fatty acid structure affects cellular activities through changes in membrane lipid composition and the generation of a diversity of bioactive derivatives. Eicosapolyenoic acids are released into plants upon infection by oomycete pathogens, suggesting they may elicit plant defenses. We exploited transgenic Arabidopsis thaliana plants (designated EP) producing eicosadienoic, eicosatrienoic, and arachidonic acid (AA), aimed at mimicking pathogen release of these compounds. We also examined their effect on biotic stress resistance by challenging EP plants with fungal, oomycete, and bacterial pathogens and an insect pest. EP plants exhibited enhanced resistance to all biotic challenges, except they were more susceptible to bacteria than the wild type. Levels of jasmonic acid (JA) were elevated and levels of salicylic acid (SA) were reduced in EP plants. Altered expression of JA and SA pathway genes in EP plants shows that eicosapolyenoic acids effectively modulate stress-responsive transcriptional networks. Exogenous application of various fatty acids to wild-type and JA-deficient mutants confirmed AA as the signaling molecule. Moreover, AA treatment elicited heightened expression of general stress-responsive genes. Importantly, tomato (Solanum lycopersicum) leaves treated with AA exhibited reduced susceptibility to Botrytis cinerea infection, confirming AA signaling in other plants. These studies support the role of AA, an ancient metazoan signaling molecule, in eliciting plant stress and defense signaling networks. PMID:20935246

  6. Metrics and methods for characterizing dairy farm intensification using farm survey data.

    PubMed

    Gonzalez-Mejia, Alejandra; Styles, David; Wilson, Paul; Gibbons, James

    2018-01-01

    Evaluation of agricultural intensification requires comprehensive analysis of trends in farm performance across physical and socio-economic aspects, which may diverge across farm types. Typical reporting of economic indicators at sectorial or the "average farm" level does not represent farm diversity and provides limited insight into the sustainability of specific intensification pathways. Using farm business data from a total of 7281 farm survey observations of English and Welsh dairy farms over a 14-year period we calculate a time series of 16 key performance indicators (KPIs) pertinent to farm structure, environmental and socio-economic aspects of sustainability. We then apply principle component analysis and model-based clustering analysis to identify statistically the number of distinct dairy farm typologies for each year of study, and link these clusters through time using multidimensional scaling. Between 2001 and 2014, dairy farms have largely consolidated and specialized into two distinct clusters: more extensive farms relying predominantly on grass, with lower milk yields but higher labour intensity, and more intensive farms producing more milk per cow with more concentrate and more maize, but lower labour intensity. There is some indication that these clusters are converging as the extensive cluster is intensifying slightly faster than the intensive cluster, in terms of milk yield per cow and use of concentrate feed. In 2014, annual milk yields were 6,835 and 7,500 l/cow for extensive and intensive farm types, respectively, whilst annual concentrate feed use was 1.3 and 1.5 tonnes per cow. For several KPIs such as milk yield the mean trend across all farms differed substantially from the extensive and intensive typologies mean. The indicators and analysis methodology developed allows identification of distinct farm types and industry trends using readily available survey data. The identified groups allow the accurate evaluation of the consequences of the

  7. Effects of fish farming on phytoplankton community under the thermal stress caused by a power plant in a eutrophic, semi-enclosed bay: induce toxic dinoflagellate (Prorocentrum minimum) blooms in cold seasons.

    PubMed

    Jiang, Zhibing; Liao, Yibo; Liu, Jingjing; Shou, Lu; Chen, Quanzhen; Yan, Xiaojun; Zhu, Genhai; Zeng, Jiangning

    2013-11-15

    Six cruises were conducted in a fish farm adjacent to the Ninghai Power Plant in Xiangshan Bay, East China Sea. Fish farming significantly increased NH4(+), DIP, and TOC concentrations, while it significantly decreased the DO level. These increase/decrease trends were more pronounced in warmer seasons. Although culture practices did not significantly increase phytoplankton density, it drastically enhanced dinoflagellate abundance and domination. Significant differences in species diversity and community composition between the cages and the control area were also observed. Temperature elevation caused by thermal discharge associated with eutrophication resulted in a dominant species shift from diatoms alone to dinoflagellates and diatoms. This is the first report of stress-induced toxic dinoflagellate (Prorocentrum minimum) blooms in winter and the winter-spring transition in this bay. Therefore, the effects of aquaculture activity and power plant construction in such a eutrophic, semi-enclosed bay require further attention. Copyright © 2013 Elsevier Ltd. All rights reserved.

  8. Whiteflies interfere with indirect plant defense against spider mites in Lima bean

    PubMed Central

    Zhang, Peng-Jun; Zheng, Si-Jun; van Loon, Joop J. A.; Boland, Wilhelm; David, Anja; Mumm, Roland; Dicke, Marcel

    2009-01-01

    Plants under herbivore attack are able to initiate indirect defense by synthesizing and releasing complex blends of volatiles that attract natural enemies of the herbivore. However, little is known about how plants respond to infestation by multiple herbivores, particularly if these belong to different feeding guilds. Here, we report the interference by a phloem-feeding insect, the whitefly Bemisia tabaci, with indirect plant defenses induced by spider mites (Tetranychus urticae) in Lima bean (Phaseolus lunatus) plants. Additional whitefly infestation of spider-mite infested plants resulted in a reduced attraction of predatory mites (Phytoseiulus persimilis) compared to attraction to plants infested by spider mites only. This interference is shown to result from the reduction in (E)-β-ocimene emission from plants infested by both spider mites and whiteflies. When using exogenous salicylic acid (SA) application to mimic B. tabaci infestation, we observed similar results in behavioral and chemical analyses. Phytohormone and gene-expression analyses revealed that B. tabaci infestation, as well as SA application, inhibited spider mite-induced jasmonic acid (JA) production and reduced the expression of two JA-regulated genes, one of which encodes for the P. lunatus enzyme β-ocimene synthase that catalyzes the synthesis of (E)-β-ocimene. Remarkably, B. tabaci infestation concurrently inhibited SA production induced by spider mites. We therefore conclude that in dual-infested Lima bean plants the suppression of the JA signaling pathway by whitefly feeding is not due to enhanced SA levels. PMID:19965373

  9. Transcriptome Analysis of ABA/JA-Dual Responsive Genes in Rice Shoot and Root.

    PubMed

    Kim, Jin-Ae; Bhatnagar, Nikita; Kwon, Soon Jae; Min, Myung Ki; Moon, Seok-Jun; Yoon, In Sun; Kwon, Taek-Ryoun; Kim, Sun Tae; Kim, Beom-Gi

    2018-01-01

    The phytohormone abscisic acid (ABA) enables plants to adapt to adverse environmental conditions through the modulation of metabolic pathways and of growth and developmental programs. We used comparative microarray analysis to identify genes exhibiting ABA-dependent expression and other hormone-dependent expression among them in Oryza sativa shoot and root. We identified 854 genes as significantly up- or down-regulated in root or shoot under ABA treatment condition. Most of these genes had similar expression profiles in root and shoot under ABA treatment condition, whereas 86 genes displayed opposite expression responses in root and shoot. To examine the crosstalk between ABA and other hormones, we compared the expression profiles of the ABA-dependently regulated genes under several different hormone treatment conditions. Interestingly, around half of the ABA-dependently expressed genes were also regulated by jasmonic acid based on microarray data analysis. We searched the promoter regions of these genes for cis-elements that could be responsible for their responsiveness to both hormones, and found that ABRE and MYC2 elements, among others, were common to the promoters of genes that were regulated by both ABA and JA. These results show that ABA and JA might have common gene expression regulation system and might explain why the JA could function for both abiotic and biotic stress tolerance.

  10. Organic Farming: Biodiversity Impacts Can Depend on Dispersal Characteristics and Landscape Context

    PubMed Central

    Feber, Ruth E.; Johnson, Paul J.; Bell, James R.; Chamberlain, Dan E.; Firbank, Leslie G.; Fuller, Robert J.; Manley, Will; Mathews, Fiona; Norton, Lisa R.; Townsend, Martin; Macdonald, David W.

    2015-01-01

    Organic farming, a low intensity system, may offer benefits for a range of taxa, but what affects the extent of those benefits is imperfectly understood. We explored the effects of organic farming and landscape on the activity density and species density of spiders and carabid beetles, using a large sample of paired organic and conventional farms in the UK. Spider activity density and species density were influenced by both farming system and surrounding landscape. Hunting spiders, which tend to have lower dispersal capabilities, had higher activity density, and more species were captured, on organic compared to conventional farms. There was also evidence for an interaction, as the farming system effect was particularly marked in the cropped area before harvest and was more pronounced in complex landscapes (those with little arable land). There was no evidence for any effect of farming system or landscape on web-building spiders (which include the linyphiids, many of which have high dispersal capabilities). For carabid beetles, the farming system effects were inconsistent. Before harvest, higher activity densities were observed in the crops on organic farms compared with conventional farms. After harvest, no difference was detected in the cropped area, but more carabids were captured on conventional compared to organic boundaries. Carabids were more species-dense in complex landscapes, and farming system did not affect this. There was little evidence that non-cropped habitat differences explained the farming system effects for either spiders or carabid beetles. For spiders, the farming system effects in the cropped area were probably largely attributable to differences in crop management; reduced inputs of pesticides (herbicides and insecticides) and fertilisers are possible influences, and there was some evidence for an effect of non-crop plant species richness on hunting spider activity density. The benefits of organic farming may be greatest for taxa with lower

  11. Organic Farming: Biodiversity Impacts Can Depend on Dispersal Characteristics and Landscape Context.

    PubMed

    Feber, Ruth E; Johnson, Paul J; Bell, James R; Chamberlain, Dan E; Firbank, Leslie G; Fuller, Robert J; Manley, Will; Mathews, Fiona; Norton, Lisa R; Townsend, Martin; Macdonald, David W

    2015-01-01

    Organic farming, a low intensity system, may offer benefits for a range of taxa, but what affects the extent of those benefits is imperfectly understood. We explored the effects of organic farming and landscape on the activity density and species density of spiders and carabid beetles, using a large sample of paired organic and conventional farms in the UK. Spider activity density and species density were influenced by both farming system and surrounding landscape. Hunting spiders, which tend to have lower dispersal capabilities, had higher activity density, and more species were captured, on organic compared to conventional farms. There was also evidence for an interaction, as the farming system effect was particularly marked in the cropped area before harvest and was more pronounced in complex landscapes (those with little arable land). There was no evidence for any effect of farming system or landscape on web-building spiders (which include the linyphiids, many of which have high dispersal capabilities). For carabid beetles, the farming system effects were inconsistent. Before harvest, higher activity densities were observed in the crops on organic farms compared with conventional farms. After harvest, no difference was detected in the cropped area, but more carabids were captured on conventional compared to organic boundaries. Carabids were more species-dense in complex landscapes, and farming system did not affect this. There was little evidence that non-cropped habitat differences explained the farming system effects for either spiders or carabid beetles. For spiders, the farming system effects in the cropped area were probably largely attributable to differences in crop management; reduced inputs of pesticides (herbicides and insecticides) and fertilisers are possible influences, and there was some evidence for an effect of non-crop plant species richness on hunting spider activity density. The benefits of organic farming may be greatest for taxa with lower

  12. Farm Population Trends and Farm Characteristics. Rural Development Research Report No. 3.

    ERIC Educational Resources Information Center

    Banks, Vera J.

    While total farm population is declining, the number of people living on the farms which produce the bulk of the nation's food and fiber is increasing. The 1970-75 total farm population decline was 13 percent, but the number of people living on farms with annual sales greater than $40,000 increased 76 percent. Such farms account for about 80…

  13. Farm-Size Structure and Off-Farm Income and Employment Generation in the North Central Region.

    ERIC Educational Resources Information Center

    Heady, Earl O.; Sonka, Steven T.

    The relationship between size of farm and the welfare of farm and nonfarm society was examined in terms of total income in the farm sector, the number and size of farms, income per farm, secondary income generation, and consumer food costs using four alternative farm structures: large farm (gross farm sales of at least $40,000); medium farm (gross…

  14. What Happens to the Farm? Australian Farmers' Experiences after a Serious Farm Injury.

    PubMed

    Beattie, Jessica; McLeod, Candis; Murray, Meg; Pedler, Daryl; Brumby, Susan; Gabbe, Belinda

    2018-01-01

    To investigate the experiences of farmers on returning to farming following a serious farm-related injury. Patients who had sustained major trauma on a farm in Victoria, Australia, were identified using the Victorian State Trauma Registry (VSTR). In depth, semistructured phone interviews were conducted with 31 participants. Interview data were subjected to thematic analysis to identify important recurring themes. Interviews continued until data saturation was achieved. Interviewees included self-employed full-time farmers, part-time farmers with a supplementary income, and agricultural employees. Analysis of participant responses connected to returning to farming after a serious farm-related injury identified five major interconnected themes: (i) effect on farm work, (ii) farming future, (iii) safety advocacy, (iv) changes to farming practices, and (v) financial ramifications. Farmers who have sustained a serious farm-related injury are an important resource; their experiences and perspectives could assist in the development of educational and transitional support services from recovery back to working at a preinjury level, while ensuring farming production is sustainable during this period. Furthermore, farm safety programs can be enhanced by the engagement of farmers, such as participants in this study as advocates for improved farm safety practices.

  15. Phytoplasma protein effector SAP11 enhances insect vector reproduction by manipulating plant development and defense hormone biosynthesis.

    PubMed

    Sugio, Akiko; Kingdom, Heather N; MacLean, Allyson M; Grieve, Victoria M; Hogenhout, Saskia A

    2011-11-29

    Phytoplasmas are insect-transmitted phytopathogenic bacteria that can alter plant morphology and the longevity and reproduction rates and behavior of their insect vectors. There are various examples of animal and plant parasites that alter the host phenotype to attract insect vectors, but it is unclear how these parasites accomplish this. We hypothesized that phytoplasmas produce effectors that modulate specific targets in their hosts leading to the changes in plant development and insect performance. Previously, we sequenced and mined the genome of Aster Yellows phytoplasma strain Witches' Broom (AY-WB) and identified 56 candidate effectors. Here, we report that the secreted AY-WB protein 11 (SAP11) effector modulates plant defense responses to the advantage of the AY-WB insect vector Macrosteles quadrilineatus. SAP11 binds and destabilizes Arabidopsis CINCINNATA (CIN)-related TEOSINTE BRANCHED1, CYCLOIDEA, PROLIFERATING CELL FACTORS 1 and 2 (TCP) transcription factors, which control plant development and promote the expression of lipoxygenase (LOX) genes involved in jasmonate (JA) synthesis. Both the Arabidopsis SAP11 lines and AY-WB-infected plants produce less JA on wounding. Furthermore, the AY-WB insect vector produces more offspring on AY-WB-infected plants, SAP11 transgenic lines, and plants impaired in CIN-TCP and JA synthesis. Thus, SAP11-mediated destabilization of CIN-TCPs leads to the down-regulation of LOX2 expression and JA synthesis and an increase in M. quadrilineatus progeny. Phytoplasmas are obligate inhabitants of their plant host and insect vectors, in which the latter transmits AY-WB to a diverse range of plant species. This finding demonstrates that pathogen effectors can reach beyond the pathogen-host interface to modulate a third organism in the biological interaction.

  16. Metrics and methods for characterizing dairy farm intensification using farm survey data

    PubMed Central

    Gonzalez-Mejia, Alejandra; Styles, David; Wilson, Paul

    2018-01-01

    Evaluation of agricultural intensification requires comprehensive analysis of trends in farm performance across physical and socio-economic aspects, which may diverge across farm types. Typical reporting of economic indicators at sectorial or the “average farm” level does not represent farm diversity and provides limited insight into the sustainability of specific intensification pathways. Using farm business data from a total of 7281 farm survey observations of English and Welsh dairy farms over a 14-year period we calculate a time series of 16 key performance indicators (KPIs) pertinent to farm structure, environmental and socio-economic aspects of sustainability. We then apply principle component analysis and model-based clustering analysis to identify statistically the number of distinct dairy farm typologies for each year of study, and link these clusters through time using multidimensional scaling. Between 2001 and 2014, dairy farms have largely consolidated and specialized into two distinct clusters: more extensive farms relying predominantly on grass, with lower milk yields but higher labour intensity, and more intensive farms producing more milk per cow with more concentrate and more maize, but lower labour intensity. There is some indication that these clusters are converging as the extensive cluster is intensifying slightly faster than the intensive cluster, in terms of milk yield per cow and use of concentrate feed. In 2014, annual milk yields were 6,835 and 7,500 l/cow for extensive and intensive farm types, respectively, whilst annual concentrate feed use was 1.3 and 1.5 tonnes per cow. For several KPIs such as milk yield the mean trend across all farms differed substantially from the extensive and intensive typologies mean. The indicators and analysis methodology developed allows identification of distinct farm types and industry trends using readily available survey data. The identified groups allow the accurate evaluation of the consequences of

  17. The farm apprentice: agricultural college students recollections of learning to farm "safely".

    PubMed

    Sanderson, L L; Dukeshire, S R; Rangel, C; Garbes, R

    2010-10-01

    A consistent message in the farm safety literature is the need to develop effective interventions to manage the unacceptably high rate of injury and death among farm children. To better understand the influence of childhood farm experiences on safety beliefs, attitudes, and practices, semi-structured interviews were conducted with 24 farm youth attending the Nova Scotia Agricultural College. The interviews were designed to elicit information pertaining to participants' earliest memories of involvement in farm activities, the decision-making processes that led them to assume work-related responsibilities, and the roles that their parents played in their safety training. A common theme of experiencing childhood as a "farm apprentice" emerged across all narratives whereby farm activities were learned primarily through observational learning and modeling of parents and then mastered through repetition. As "farm apprentices," the youths' involvement in dangerous activities such as tractor driving and livestock handling began at early ages, with very little formal training and supervision. Although participants clearly described themselves as being exposed to dangerous activities, they believed that they had the capacity to control the risks and farm safely. Based on our findings, the concept of the "farm apprentice" appears to be integral to the social context of the farming community and should be considered in the design of interventions to reduce child injury and death.

  18. Expressing OsMPK4 Impairs Plant Growth but Enhances the Resistance of Rice to the Striped Stem Borer Chilo suppressalis

    PubMed Central

    Liu, Xiaoli; Li, Jiancai; Xu, Liping; Wang, Qi

    2018-01-01

    Mitogen-activated protein kinases (MPKs) play a central role not only in plant growth and development, but also in plant responses to abiotic and biotic stresses, including pathogens. Yet, their role in herbivore-induced plant defenses and their underlying mechanisms remain largely unknown. Here, we cloned a rice MPK gene, OsMPK4, whose expression was induced by mechanical wounding, infestation of the striped stem borer (SSB) Chilo suppressalis, and treatment with jasmonic acid (JA), but not by treatment with salicylic acid (SA). The overexpression of OsMPK4 (oe-MPK4) enhanced constitutive and/or SSB-induced levels of JA, jasmonoyl-l-isoleucine (JA-Ile), ethylene (ET), and SA, as well as the activity of elicited trypsin proteinase inhibitors (TrypPIs), and reduced SSB performance. On the other hand, compared to wild-type plants, oe-MPK4 lines in the greenhouse showed growth retardation. These findings suggest that OsMPK4, by regulating JA-, ET-, and SA-mediated signaling pathways, functions as a positive regulator of rice resistance to the SSB and a negative regulator of rice growth. PMID:29652796

  19. GA3 and other signal regulators (MeJA and IAA) improve xanthumin biosynthesis in different manners in Xanthium strumarium L.

    PubMed

    Li, Changfu; Chen, Fangfang; Zhang, Yansheng

    2014-08-25

    Xanthanolides from Xanthium strumarium L. exhibit various pharmacological activities and these compounds are mainly produced in the glandular trichomes of aerial plant parts. The regulation of xanthanolide biosynthesis has never been reported in the literature. In this study, the effects of phytohormonal stimulation on xanthumin (a xanthanolide compound) biosynthesis, glandular trichomes and germacrene A synthase (GAS) gene expression in X. strumarium L. young leaves were investigated. The exogenous applications of methyl jasmonate (MeJA), indole-3-acetic acid (IAA), and gibberrellin A3 (GA3) at appropriate concentrations were all found to improve xanthumin biosynthesis, but in different ways. It was suggested that a higher gland density stimulated by MeJA (400 µM) or IAA (200 µM) treatment caused at least in part an improvement in xanthumin production, whereas GA3 (10 µM) led to an improvement by up-regulating xanthumin biosynthetic genes within gland cells, not by forming more glandular trichomes. Compared to the plants before the flowering stage, plants that had initiated flowering showed enhanced xanthumin biosynthesis, but no higher gland density, an effect was similar to that caused by exogenous GA3 treatment.

  20. Whole Farm Net Greenhouse Gas Abatement from Establishing Kikuyu-Based Perennial Pastures in South-Western Australia

    PubMed Central

    Thomas, Dean T.; Sanderman, Jonathan; Eady, Sandra J.; Masters, David G.; Sanford, Paul

    2012-01-01

    Simple Summary Greenhouse gas (GHG) emissions from ruminant livestock production (sheep, cattle and goats) have contributed to a common perception that a shift in the human diet from animal to plant-based products is environmentally responsible. In this study we found that the level of net emissions from livestock production systems is strongly influenced by the type of farming system that is used, and in fact GHG emission levels from some livestock production systems may be comparable with cropping systems. By introducing into farming systems ‘perennial’ pasture plants that are able to capture more atmospheric carbon, which is then stored in the soil, emission levels from livestock production can be substantially reduced. Abstract On-farm activities that reduce GHG emissions or sequester carbon from the atmosphere to compensate for anthropogenic emissions are currently being evaluated by the Australian Government as carbon offset opportunities. The aim of this study was to examine the implications of establishing and grazing Kikuyu pastures, integrated as part of a mixed Merino sheep and cropping system, as a carbon offset mechanism. For the assessment of changes in net greenhouse gas emissions, results from a combination of whole farm economic and livestock models were used (MIDAS and GrassGro). Net GHG emissions were determined by deducting increased emissions from introducing this practice change (increased methane and nitrous oxide emissions due to higher stocking rates) from the soil carbon sequestered from growing the Kikuyu pasture. Our results indicate that livestock systems using perennial pastures may have substantially lower net GHG emissions, and reduced GHG intensity of production, compared with annual plant-based production systems. Soil carbon accumulation by converting 45% of arable land within a farm enterprise to Kikuyu-based pasture was determined to be 0.80 t CO2-e farm ha−1 yr−1 and increased GHG emissions (leakage) was 0.19 t CO2-e

  1. UV-C–Irradiated Arabidopsis and Tobacco Emit Volatiles That Trigger Genomic Instability in Neighboring Plants[W

    PubMed Central

    Yao, Youli; Danna, Cristian H.; Zemp, Franz J.; Titov, Viktor; Ciftci, Ozan Nazim; Przybylski, Roman; Ausubel, Frederick M.; Kovalchuk, Igor

    2011-01-01

    We have previously shown that local exposure of plants to stress results in a systemic increase in genome instability. Here, we show that UV-C–irradiated plants produce a volatile signal that triggers an increase in genome instability in neighboring nonirradiated Arabidopsis thaliana plants. This volatile signal is interspecific, as UV-C–irradiated Arabidopsis plants transmit genome destabilization to naive tobacco (Nicotiana tabacum) plants and vice versa. We report that plants exposed to the volatile hormones methyl salicylate (MeSA) or methyl jasmonate (MeJA) exhibit a similar level of genome destabilization as UV-C–irradiated plants. We also found that irradiated Arabidopsis plants produce MeSA and MeJA. The analysis of mutants impaired in the synthesis and/or response to salicylic acid (SA) and/or jasmonic acid showed that at least one other volatile compound besides MeSA and MeJA can communicate interplant genome instability. The NONEXPRESSOR OF PATHOGENESIS-RELATED GENES1 (npr1) mutant, defective in SA signaling, is impaired in both the production and the perception of the volatile signals, demonstrating a key role for NPR1 as a central regulator of genome stability. Finally, various forms of stress resulting in the formation of necrotic lesions also generate a volatile signal that leads to genomic instability. PMID:22028460

  2. Contrasting effects of biochar, compost and farm manure on alleviation of nickel toxicity in maize (Zea mays L.) in relation to plant growth, photosynthesis and metal uptake.

    PubMed

    Rehman, Muhammad Zia-Ur; Rizwan, Muhammad; Ali, Shafaqat; Fatima, Nida; Yousaf, Balal; Naeem, Asif; Sabir, Muhammad; Ahmad, Hamaad Raza; Ok, Yong Sik

    2016-11-01

    Nickel (Ni) toxicity in agricultural crops is a widespread problem while little is known about the role of biochar (BC) and other organic amendments like farm manure (FM) from cattle farm and compost (Cmp) on its alleviation. A greenhouse experiment was conducted to evaluate the effects of BC, Cmp and FM on physiological and biochemical characteristics of maize (Zea mays L.) under Ni stress. Maize was grown in Ni spiked soil without and with two rates of the amendments (equivalent to 1% and 2% organic carbon, OC) applied separately to the soil. After harvest, plant height, root length, dry weight, chlorophyll contents, gas exchange characteristics and trace elements in plants were determined. In addition, post-harvest soil characteristics like pHs, ECe and bioavailable Ni were also determined. Compared to the control, all of the amendments increased plant height, root length, shoot and root dry weight with the maximum increase in all parameters by FM (2% OC) treatment. Similarly, total chlorophyll contents and gas exchange characteristics significantly increased with the application of amendments being maximum with FM (2% OC) application. Amendments significantly increased copper, zinc, manganese and iron concentrations and decreased Ni concentrations in the plants. The highest reduction in shoot Ni concentration was recorded with FM (2% OC) followed by BC (2% OC) being 73.2% and 61.1% lower compared to the control, respectively. The maximum increase in soil pH and decrease in AB-DTPA extractable Ni was recorded with BC (2% OC) followed by FM (2% OC). It is concluded that FM (2% OC) was the most effective in reducing Ni toxicity to plants by reducing Ni uptake while BC (2% OC) was the most effective in decreasing bioavailable Ni in the soil through increasing soil pH. However, long-term field studies are needed to evaluate the effects of these amendments in reducing Ni toxicity in plants. Copyright © 2016 Elsevier Inc. All rights reserved.

  3. The Rapid Adjustment Farm Program's Influence on Other Farms in the Community.

    ERIC Educational Resources Information Center

    Simeral, Kenneth D.

    The study investigated the diffusion of innovative farming practices from Rapid Adjustment Farms (RAF) to other farms in southeast Ohio. The RAF program, begun in 1968, introduced new technology and management practices to its participant farmers. After reviewing literature of farming programs' information diffusion, a descriptive survey was made…

  4. 50 CFR 14.23 - Live farm-raised fish and farm-raised fish eggs.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 50 Wildlife and Fisheries 1 2013-10-01 2013-10-01 false Live farm-raised fish and farm-raised fish eggs. 14.23 Section 14.23 Wildlife and Fisheries UNITED STATES FISH AND WILDLIFE SERVICE, DEPARTMENT OF... Exportation at Designated Ports § 14.23 Live farm-raised fish and farm-raised fish eggs. Live farm-raised fish...

  5. 50 CFR 14.23 - Live farm-raised fish and farm-raised fish eggs.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 50 Wildlife and Fisheries 1 2014-10-01 2014-10-01 false Live farm-raised fish and farm-raised fish eggs. 14.23 Section 14.23 Wildlife and Fisheries UNITED STATES FISH AND WILDLIFE SERVICE, DEPARTMENT OF... Exportation at Designated Ports § 14.23 Live farm-raised fish and farm-raised fish eggs. Live farm-raised fish...

  6. 50 CFR 14.23 - Live farm-raised fish and farm-raised fish eggs.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 50 Wildlife and Fisheries 1 2012-10-01 2012-10-01 false Live farm-raised fish and farm-raised fish eggs. 14.23 Section 14.23 Wildlife and Fisheries UNITED STATES FISH AND WILDLIFE SERVICE, DEPARTMENT OF... Exportation at Designated Ports § 14.23 Live farm-raised fish and farm-raised fish eggs. Live farm-raised fish...

  7. Long-chain omega-3 from low-trophic-level fish provides value to farmed seafood.

    PubMed

    Bibus, Douglas M

    2015-03-01

    Low-trophic-level fish are a crucial source of long-chain (LC) omega-3 fatty acids for farmed fish and humans. Many farm-raised fish species have a clear need for these nutrients. Farmed fish deposit the LC omega-3s in their flesh and transfer them up the food chain. However, the content of LC omega-3s in farm-raised seafood continues to decline, while the content of shorter-chain plant-sourced omega-3s, and pro-inflammtory omega-6s continue to increase. This reduces its nutritional worth. The value of low-trophic-level fish is often viewed merely as its price at the dock. Some reports and metrics steer public attention towards the mass balance between quantities of low-trophic-level fish and farmed seafood. However, the the nutritional value of seafood is more important than its mere quantities. The role of low-trophic-level fish in human nutrition, health, and wellbeing is a fundamental component of its economic value to society.

  8. Farm Population of the United States: 1975. Current Population Reports: Farm Population.

    ERIC Educational Resources Information Center

    Banks, Vera J.; And Others

    Presented via narrative and tabular data, this report includes: Population of the U.S., Total and Farm: 1960-1975; Metropolitan-Nonmetropolitan Residence of the Farm and Nonfarm Population, by Race: 1975; Fertility Characteristics of Farm and Nonfarm Women, by Race: 1975; Persons 14 Years Old and Over Employed in Agriculture, by Farm-Nonfarm…

  9. Role of Tomato Lipoxygenase D in Wound-Induced Jasmonate Biosynthesis and Plant Immunity to Insect Herbivores

    PubMed Central

    Li, Shuyu; Wang, Bao; Huang, Tingting; Du, Minmin; Sun, Jiaqiang; Kang, Le; Li, Chang-Bao; Li, Chuanyou

    2013-01-01

    In response to insect attack and mechanical wounding, plants activate the expression of genes involved in various defense-related processes. A fascinating feature of these inducible defenses is their occurrence both locally at the wounding site and systemically in undamaged leaves throughout the plant. Wound-inducible proteinase inhibitors (PIs) in tomato (Solanum lycopersicum) provide an attractive model to understand the signal transduction events leading from localized injury to the systemic expression of defense-related genes. Among the identified intercellular molecules in regulating systemic wound response of tomato are the peptide signal systemin and the oxylipin signal jasmonic acid (JA). The systemin/JA signaling pathway provides a unique opportunity to investigate, in a single experimental system, the mechanism by which peptide and oxylipin signals interact to coordinate plant systemic immunity. Here we describe the characterization of the tomato suppressor of prosystemin-mediated responses8 (spr8) mutant, which was isolated as a suppressor of (pro)systemin-mediated signaling. spr8 plants exhibit a series of JA-dependent immune deficiencies, including the inability to express wound-responsive genes, abnormal development of glandular trichomes, and severely compromised resistance to cotton bollworm (Helicoverpa armigera) and Botrytis cinerea. Map-based cloning studies demonstrate that the spr8 mutant phenotype results from a point mutation in the catalytic domain of TomLoxD, a chloroplast-localized lipoxygenase involved in JA biosynthesis. We present evidence that overexpression of TomLoxD leads to elevated wound-induced JA biosynthesis, increased expression of wound-responsive genes and, therefore, enhanced resistance to insect herbivory attack and necrotrophic pathogen infection. These results indicate that TomLoxD is involved in wound-induced JA biosynthesis and highlight the application potential of this gene for crop protection against insects and

  10. Role of tomato lipoxygenase D in wound-induced jasmonate biosynthesis and plant immunity to insect herbivores.

    PubMed

    Yan, Liuhua; Zhai, Qingzhe; Wei, Jianing; Li, Shuyu; Wang, Bao; Huang, Tingting; Du, Minmin; Sun, Jiaqiang; Kang, Le; Li, Chang-Bao; Li, Chuanyou

    2013-01-01

    In response to insect attack and mechanical wounding, plants activate the expression of genes involved in various defense-related processes. A fascinating feature of these inducible defenses is their occurrence both locally at the wounding site and systemically in undamaged leaves throughout the plant. Wound-inducible proteinase inhibitors (PIs) in tomato (Solanum lycopersicum) provide an attractive model to understand the signal transduction events leading from localized injury to the systemic expression of defense-related genes. Among the identified intercellular molecules in regulating systemic wound response of tomato are the peptide signal systemin and the oxylipin signal jasmonic acid (JA). The systemin/JA signaling pathway provides a unique opportunity to investigate, in a single experimental system, the mechanism by which peptide and oxylipin signals interact to coordinate plant systemic immunity. Here we describe the characterization of the tomato suppressor of prosystemin-mediated responses8 (spr8) mutant, which was isolated as a suppressor of (pro)systemin-mediated signaling. spr8 plants exhibit a series of JA-dependent immune deficiencies, including the inability to express wound-responsive genes, abnormal development of glandular trichomes, and severely compromised resistance to cotton bollworm (Helicoverpa armigera) and Botrytis cinerea. Map-based cloning studies demonstrate that the spr8 mutant phenotype results from a point mutation in the catalytic domain of TomLoxD, a chloroplast-localized lipoxygenase involved in JA biosynthesis. We present evidence that overexpression of TomLoxD leads to elevated wound-induced JA biosynthesis, increased expression of wound-responsive genes and, therefore, enhanced resistance to insect herbivory attack and necrotrophic pathogen infection. These results indicate that TomLoxD is involved in wound-induced JA biosynthesis and highlight the application potential of this gene for crop protection against insects and

  11. Crosstalk among Jasmonate, Salicylate and Ethylene Signaling Pathways in Plant Disease and Immune Responses.

    PubMed

    Yang, You-Xin; Ahammed, Golam J; Wu, Caijun; Fan, Shu-ying; Zhou, Yan-Hong

    2015-01-01

    Phytohormone crosstalk is crucial for plant defenses against pathogens and insects in which salicylic acid (SA), jasmonic acid (JA), and ethylene (ET) play key roles. These low molecular mass signals critically trigger and modulate plant resistance against biotrophic as well as necrotrophic pathogens through a complex signaling network that even involves participation of other hormones. Crosstalk among SA, JA and ET is mediated by different molecular players, considered as integral part of these crosscommunicating signal transduction pathways. Recent progress has revealed that the positive versus negative interactions among those pathways ultimately enable a plant to fine-tune its defense against specific aggressors. On the other hand, pathogens have evolved strategies to manipulate the signaling network to their favour in order to intensify virulence on host plant. Here we review recent advances and current knowledge on the role of classical primary defense hormones SA, JA and ET as well as their synergistic and antagonistic interaction in plant disease and immune responses. Crosstalk with other hormones such as abscisic acid, auxin, brassinosteroids, cytokinins and melatonin is also discussed mainly in plant disease resistance. In addition to our keen focus on hormonal crosstalk, this review also highlights potential implication of positive and negative regulatory interactions for developing an efficient disease management strategy through manipulation of hormone signaling in plant.

  12. Radium and uranium levels in vegetables grown using different farming management systems.

    PubMed

    Lauria, D C; Ribeiro, F C A; Conti, C C; Loureiro, F A

    2009-02-01

    Vegetables grown with phosphate fertilizer (conventional management), with bovine manure fertilization (organic management) and in a mineral nutrient solution (hydroponic) were analyzed and the concentrations of (238)U, (226)Ra and (228)Ra in lettuce, carrots, and beans were compared. Lettuce from hydroponic farming system showed the lowest concentration of radionuclides 0.51 for (226)Ra, 0.55 for (228)Ra and 0.24 for (238)U (Bq kg(-1) dry). Vegetables from organically and conventionally grown farming systems showed no differences in the concentration of radium and uranium. Relationships between uranium content in plants and exchangeable Ca and Mg in soil were found, whereas Ra in vegetables was inversely correlated to the cation exchange capacity of soil, leading to the assumption that by supplying carbonate and cations to soil, liming may cause an increase of U and a decrease of radium uptake by plants. The soil to plant transfer varied from 10(-4) to 10(-2) for (238)U and from 10(-2) to 10(-1) for (228)Ra.

  13. Potential for seed-mediated gene flow in agroecosystems from transgenic safflower (Carthamus tinctorius L.) intended for plant molecular farming.

    PubMed

    McPherson, Marc A; Yang, Rong-Cai; Good, Allen G; Nielson, Ryan L; Hall, Linda M

    2009-04-01

    Safflower has been transformed for field scale molecular farming of high-value proteins including several pharmaceuticals. Viable safflower seed remaining in the soil seed bank after harvest could facilitate seed and pollen-mediated gene flow. Seeds may germinate in subsequent years and volunteer plants may flower and potentially outcross with commodity safflower and/or produce seed. Seeds from volunteers could become admixed with conventional crops at harvest, and/or replenish the seed bank. Seed in following crops could be transported locally and internationally and facilitate gene flow in locations where regulatory thresholds and public acceptance differ from Canada. Seed-mediated gene flow was examined in three studies. Safflower seed loss and viability following harvest of commercial fields of a non-transgenic cultivar were determined. We assessed seed longevity of transgenic and non-transgenic safflower, on the soil surface and buried at two depths. Finally, we surveyed commercial safflower fields at different sites and measured density and growth stage of safflower volunteers, in other crops the following year and documented volunteer survival and viable seed production. Total seed loss at harvest in commercial fields, ranged from 231 to 1,069 seeds m(-2) and the number of viable seeds ranged from 81 to 518 seeds m(-2). Safflower has a relatively short longevity in the seed bank and no viable seeds were found after 2 years. Based on the seed burial studies it is predicted that winter conditions would reduce safflower seed viability on the soil surface by >50%, leaving between 40 and 260 viable seeds m(-2). The density of safflower volunteers emerging in the early spring of the following year ranged from 3 to 11 seedlings m(-2). Safflower volunteers did not survive in fields under chemical fallow, but in some cereal fields small numbers of volunteers did survive and generate viable seed. Results will be used to make recommendations for best management

  14. Coordinate expression of AOS genes and JA accumulation: JA is not required for initiation of closing layer in wound healing tubers

    USDA-ARS?s Scientific Manuscript database

    Wounding induces a series of coordinated physiological responses essential for protection and healing of the damaged tissue. Wound-induced formation of jasmonic acid (JA) is important in defense responses in leaves, but comparatively little is known about the induction of JA biosynthesis and its ro...

  15. Flow adjustment inside large finite-size wind farms approaching the infinite wind farm regime

    NASA Astrophysics Data System (ADS)

    Wu, Ka Ling; Porté-Agel, Fernando

    2017-04-01

    Due to the increasing number and the growing size of wind farms, the distance among them continues to decrease. Thus, it is necessary to understand how these large finite-size wind farms and their wakes could interfere the atmospheric boundary layer (ABL) dynamics and adjacent wind farms. Fully-developed flow inside wind farms has been extensively studied through numerical simulations of infinite wind farms. The transportation of momentum and energy is only vertical and the advection of them is neglected in these infinite wind farms. However, less attention has been paid to examine the length of wind farms required to reach such asymptotic regime and the ABL dynamics in the leading and trailing edges of the large finite-size wind farms. Large eddy simulations are performed in this study to investigate the flow adjustment inside large finite-size wind farms in conventionally-neutral boundary layer with the effect of Coriolis force and free-atmosphere stratification from 1 to 5 K/km. For the large finite-size wind farms considered in the present work, when the potential temperature lapse rate is 5 K/km, the wind farms exceed the height of the ABL by two orders of magnitude for the incoming flow inside the farms to approach the fully-developed regime. An entrance fetch of approximately 40 times of the ABL height is also required for such flow adjustment. At the fully-developed flow regime of the large finite-size wind farms, the flow characteristics match those of infinite wind farms even though they have different adjustment length scales. The role of advection at the entrance and exit regions of the large finite-size wind farms is also examined. The interaction between the internal boundary layer developed above the large finite-size wind farms and the ABL under different potential temperature lapse rates are compared. It is shown that the potential temperature lapse rate plays a role in whether the flow inside the large finite-size wind farms adjusts to the fully

  16. Analysis of plant hormone profiles in response to moderate dehydration stress.

    PubMed

    Urano, Kaoru; Maruyama, Kyonoshin; Jikumaru, Yusuke; Kamiya, Yuji; Yamaguchi-Shinozaki, Kazuko; Shinozaki, Kazuo

    2017-04-01

    Plant responses to dehydration stress are mediated by highly complex molecular systems involving hormone signaling and metabolism, particularly the major stress hormone abscisic acid (ABA) and ABA-dependent gene expression. To understand the roles of plant hormones and their interactions during dehydration, we analyzed the plant hormone profiles with respect to dehydration responses in Arabidopsis thaliana wild-type (WT) plants and ABA biosynthesis mutants (nced3-2). We developed a procedure for moderate dehydration stress, and then investigated temporal changes in the profiles of ABA, jasmonic acid isoleucine (JA-Ile), salicylic acid (SA), cytokinin (trans-zeatin, tZ), auxin (indole-acetic acid, IAA), and gibberellin (GA 4 ), along with temporal changes in the expression of key genes involved in hormone biosynthesis. ABA levels increased in a bi-phasic pattern (at the early and late phases) in response to moderate dehydration stress. JA-Ile levels increased slightly in WT plants and strongly increased in nced3-2 mutant plants at 72 h after the onset of dehydration. The expression profiles of dehydration-inducible genes displayed temporal responses in an ABA-dependent manner. The early phase of ABA accumulation correlated with the expression of touch-inducible genes and was independent of factors involved in the major ABA regulatory pathway, including the ABA-responsive element-binding (AREB/ABF) transcription factor. JA-Ile, SA, and tZ were negatively regulated during the late dehydration response phase. Transcriptome analysis revealed important roles for hormone-related genes in metabolism and signaling during dehydration-induced plant responses. © 2016 The Authors The Plant Journal © 2016 John Wiley & Sons Ltd.

  17. Farming in space: environmental and biophysical concerns.

    PubMed

    Monje, O; Stutte, G W; Goins, G D; Porterfield, D M; Bingham, G E

    2003-01-01

    The colonization of space will depend on our ability to routinely provide for the metabolic needs (oxygen, water, and food) of a crew with minimal re-supply from Earth. On Earth, these functions are facilitated by the cultivation of plant crops, thus it is important to develop plant-based food production systems to sustain the presence of mankind in space. Farming practices on earth have evolved for thousands of years to meet both the demands of an ever-increasing population and the availability of scarce resources, and now these practices must adapt to accommodate the effects of global warming. Similar challenges are expected when earth-based agricultural practices are adapted for space-based agriculture. A key variable in space is gravity; planets (e.g. Mars, 1/3 g) and moons (e.g. Earth's moon, 1/6 g) differ from spacecraft orbiting the Earth (e.g. Space stations) or orbital transfer vehicles that are subject to microgravity. The movement of heat, water vapor, CO2 and O2 between plant surfaces and their environment is also affected by gravity. In microgravity, these processes may also be affected by reduced mass transport and thicker boundary layers around plant organs caused by the absence of buoyancy dependent convective transport. Future space farmers will have to adapt their practices to accommodate microgravity, high and low extremes in ambient temperatures, reduced atmospheric pressures, atmospheres containing high volatile organic carbon contents, and elevated to super-elevated CO2 concentrations. Farming in space must also be carried out within power-, volume-, and mass-limited life support systems and must share resources with manned crews. Improved lighting and sensor technologies will have to be developed and tested for use in space. These developments should also help make crop production in terrestrial controlled environments (plant growth chambers and greenhouses) more efficient and, therefore, make these alternative agricultural systems more

  18. Farming in space: Environmental and biophysical concerns

    NASA Astrophysics Data System (ADS)

    Monje, O.; Stutte, G. W.; Goins, G. D.; Porterfield, D. M.; Bingham, G. E.

    The colonization of space will depend on our ability to routinely provide for the metabolic needs (oxygen, water, and food) of a crew with minimal re-supply from Earth. On Earth, these functions are facilitated by the cultivation of plant crops, thus it is important to develop plant-based food production systems to sustain the presence of mankind in space. Farming practices on earth have evolved for thousands of years to meet both the demands of an ever-increasing population and the availability of scarce resources, and now these practices must adapt to accommodate the effects of global warming. Similar challenges are expected when earth-based agricultural practices are adapted for space-based agriculture. A key variable in space is gravity; planets (e.g. Mars, (1)/(3) g) and moons (e.g. Earth's moon, (1)/(6) g) differ from spacecraft orbiting the Earth (e.g. Space stations) or orbital transfer vehicles that are subject to microgravity. The movement of heat, water vapor, CO2 and O2 between plant surfaces and their environment is also affected by gravity. In microgravity, these processes may also be affected by reduced mass transport and thicker boundary layers around plant organs caused by the absence of buoyancy dependent convective transport. Future space farmers will have to adapt their practices to accommodate microgravity, high and low extremes in ambient temperatures, reduced atmospheric pressures, atmospheres containing high volatile organic carbon contents, and elevated to super-elevated CO2 concentrations. Farming in space must also be carried out within power-, volume-, and mass-limited life support systems and must share resources with manned crews. Improved lighting and sensor technologies will have to be developed and tested for use in space. These developments should also help make crop production in terrestrial controlled environments (plant growth chambers and greenhouses) more efficient and, therefore, make these alternative agricultural systems

  19. Farming in space: environmental and biophysical concerns

    NASA Technical Reports Server (NTRS)

    Monje, O.; Stutte, G. W.; Goins, G. D.; Porterfield, D. M.; Bingham, G. E.

    2003-01-01

    The colonization of space will depend on our ability to routinely provide for the metabolic needs (oxygen, water, and food) of a crew with minimal re-supply from Earth. On Earth, these functions are facilitated by the cultivation of plant crops, thus it is important to develop plant-based food production systems to sustain the presence of mankind in space. Farming practices on earth have evolved for thousands of years to meet both the demands of an ever-increasing population and the availability of scarce resources, and now these practices must adapt to accommodate the effects of global warming. Similar challenges are expected when earth-based agricultural practices are adapted for space-based agriculture. A key variable in space is gravity; planets (e.g. Mars, 1/3 g) and moons (e.g. Earth's moon, 1/6 g) differ from spacecraft orbiting the Earth (e.g. Space stations) or orbital transfer vehicles that are subject to microgravity. The movement of heat, water vapor, CO2 and O2 between plant surfaces and their environment is also affected by gravity. In microgravity, these processes may also be affected by reduced mass transport and thicker boundary layers around plant organs caused by the absence of buoyancy dependent convective transport. Future space farmers will have to adapt their practices to accommodate microgravity, high and low extremes in ambient temperatures, reduced atmospheric pressures, atmospheres containing high volatile organic carbon contents, and elevated to super-elevated CO2 concentrations. Farming in space must also be carried out within power-, volume-, and mass-limited life support systems and must share resources with manned crews. Improved lighting and sensor technologies will have to be developed and tested for use in space. These developments should also help make crop production in terrestrial controlled environments (plant growth chambers and greenhouses) more efficient and, therefore, make these alternative agricultural systems more

  20. From the Lab to the Farm: An Industrial Perspective of Plant Beneficial Microorganisms

    PubMed Central

    Parnell, J. Jacob; Berka, Randy; Young, Hugh A.; Sturino, Joseph M.; Kang, Yaowei; Barnhart, D. M.; DiLeo, Matthew V.

    2016-01-01

    Any successful strategy aimed at enhancing crop productivity with microbial products ultimately relies on the ability to scale at regional to global levels. Microorganisms that show promise in the lab may lack key characteristics for widespread adoption in sustainable and productive agricultural systems. This paper provides an overview of critical considerations involved with taking a strain from discovery to the farmer’s field. In addition, we review some of the most effective microbial products on the market today, explore the reasons for their success and outline some of the major challenges involved in industrial production and commercialization of beneficial strains for widespread agricultural application. General processes associated with commercializing viable microbial products are discussed in two broad categories, biofertility inoculants and biocontrol products. Specifically, we address what farmers desire in potential microbial products, how mode of action informs decisions on product applications, the influence of variation in laboratory and field study data, challenges with scaling for mass production, and the importance of consistent efficacy, product stability and quality. In order to make a significant impact on global sustainable agriculture, the implementation of plant beneficial microorganisms will require a more seamless transition between laboratory and farm application. Early attention to the challenges presented here will improve the likelihood of developing effective microbial products to improve crop yields, decrease disease severity, and help to feed an increasingly hungry planet. PMID:27540383

  1. The Combined Effects of Ethylene and MeJA on Metabolic Profiling of Phenolic Compounds in Catharanthus roseus Revealed by Metabolomics Analysis

    PubMed Central

    Liu, Jia; Liu, Yang; Wang, Yu; Zhang, Zhong-Hua; Zu, Yuan-Gang; Efferth, Thomas; Tang, Zhong-Hua

    2016-01-01

    Phenolic compounds belong to a class of secondary metabolites and are implicated in a wide range of responsive mechanisms in plants triggered by both biotic and abiotic elicitors. In this study, we approached the combinational effects of ethylene and MeJA (methyl jasmonate) on phenolic compounds profiles and gene expressions in the medicinal plant Catharanthus roseus. In virtue of a widely non-targeted metabolomics method, we identified a total of 34 kinds of phenolic compounds in the leaves, composed by 7 C6C1-, 11 C6C3-, and 16 C6C3C6 compounds. In addition, 7 kinds of intermediates critical for the biosynthesis of phenolic compounds and alkaloids were identified and discussed with phenolic metabolism. The combinational actions of ethylene and MeJA effectively promoted the total phenolic compounds, especially the C6C1 compounds (such as salicylic acid, benzoic acid) and C6C3 ones (such as cinnamic acid, sinapic acid). In contrast, the C6C3C6 compounds displayed a notably inhibitory trend in this case. Subsequently, the gene-to-metabolite networks were drawn up by searching for correlations between the expression profiles of 5 gene tags and the accumulation profiles of 41 metabolite peaks. Generally, we provide an insight into the controlling mode of ethylene-MeJA combination on phenolic metabolism in C. roseus leaves. PMID:27375495

  2. The Combined Effects of Ethylene and MeJA on Metabolic Profiling of Phenolic Compounds in Catharanthus roseus Revealed by Metabolomics Analysis.

    PubMed

    Liu, Jia; Liu, Yang; Wang, Yu; Zhang, Zhong-Hua; Zu, Yuan-Gang; Efferth, Thomas; Tang, Zhong-Hua

    2016-01-01

    Phenolic compounds belong to a class of secondary metabolites and are implicated in a wide range of responsive mechanisms in plants triggered by both biotic and abiotic elicitors. In this study, we approached the combinational effects of ethylene and MeJA (methyl jasmonate) on phenolic compounds profiles and gene expressions in the medicinal plant Catharanthus roseus. In virtue of a widely non-targeted metabolomics method, we identified a total of 34 kinds of phenolic compounds in the leaves, composed by 7 C6C1-, 11 C6C3-, and 16 C6C3C6 compounds. In addition, 7 kinds of intermediates critical for the biosynthesis of phenolic compounds and alkaloids were identified and discussed with phenolic metabolism. The combinational actions of ethylene and MeJA effectively promoted the total phenolic compounds, especially the C6C1 compounds (such as salicylic acid, benzoic acid) and C6C3 ones (such as cinnamic acid, sinapic acid). In contrast, the C6C3C6 compounds displayed a notably inhibitory trend in this case. Subsequently, the gene-to-metabolite networks were drawn up by searching for correlations between the expression profiles of 5 gene tags and the accumulation profiles of 41 metabolite peaks. Generally, we provide an insight into the controlling mode of ethylene-MeJA combination on phenolic metabolism in C. roseus leaves.

  3. Do differences in food web structure between organic and conventional farms affect the ecosystem service of pest control?

    PubMed

    Macfadyen, Sarina; Gibson, Rachel; Polaszek, Andrew; Morris, Rebecca J; Craze, Paul G; Planqué, Robert; Symondson, William O C; Memmott, Jane

    2009-03-01

    While many studies have demonstrated that organic farms support greater levels of biodiversity, it is not known whether this translates into better provision of ecosystem services. Here we use a food-web approach to analyse the community structure and function at the whole-farm scale. Quantitative food webs from 10 replicate pairs of organic and conventional farms showed that organic farms have significantly more species at three trophic levels (plant, herbivore and parasitoid) and significantly different network structure. Herbivores on organic farms were attacked by more parasitoid species on organic farms than on conventional farms. However, differences in network structure did not translate into differences in robustness to simulated species loss and we found no difference in percentage parasitism (natural pest control) across a variety of host species. Furthermore, a manipulative field experiment demonstrated that the higher species richness of parasitoids on the organic farms did not increase mortality of a novel herbivore used to bioassay ecosystem service. The explanation for these differences is likely to include inherent differences in management strategies and landscape structure between the two farming systems.

  4. Sustainable farming of the mealworm Tenebrio molitor for the production of food and feed.

    PubMed

    Grau, Thorben; Vilcinskas, Andreas; Joop, Gerrit

    2017-09-26

    The farming of edible insects is an alternative strategy for the production of protein-rich food and feed with a low ecological footprint. The industrial production of insect-derived protein is more cost-effective and energy-efficient than livestock farming or aquaculture. The mealworm Tenebrio molitor is economically among the most important species used for the large-scale conversion of plant biomass into protein. Here, we review the mass rearing of this species and its conversion into food and feed, focusing on challenges such as the contamination of food/feed products with bacteria from the insect gut and the risk of rapidly spreading pathogens and parasites. We propose solutions to prevent the outbreak of infections among farmed insects without reliance on antibiotics. Transgenerational immune priming and probiotic bacteria may provide alternative strategies for sustainable insect farming.

  5. Phytochrome regulation of plant immunity in vegetation canopies.

    PubMed

    Moreno, Javier E; Ballaré, Carlos L

    2014-07-01

    Plant immunity against pathogens and herbivores is a central determinant of plant fitness in nature and crop yield in agroecosystems. Plant immune responses are orchestrated by two key hormones: jasmonic acid (JA) and salicylic acid (SA). Recent work has demonstrated that for plants of shade-intolerant species, which include the majority of those grown as grain crops, light is a major modulator of defense responses. Light signals that indicate proximity of competitors, such as a low red to far-red (R:FR) ratio, down-regulate the expression of JA- and SA-induced immune responses against pests and pathogens. This down-regulation of defense under low R:FR ratios, which is caused by the photoconversion of the photoreceptor phytochrome B (phyB) to an inactive state, is likely to help the plant to efficiently redirect resources to rapid growth when the competition threat posed by neighboring plants is high. This review is focused on the molecular mechanisms that link phyB with defense signaling. In particular, we discuss novel signaling players that are likely to play a role in the repression of defense responses under low R:FR ratios. A better understanding of the molecular connections between photoreceptors and the hormonal regulation of plant immunity will provide a functional framework to understand the mechanisms used by plants to deal with fundamental resource allocation trade-offs under dynamic conditions of biotic stress.

  6. Forest farming practices

    Treesearch

    J.L. Chamberlain; D. Mitchell; T. Brigham; T. Hobby; L. Zabek; J. Davis

    2009-01-01

    Forest farming in North America is becoming popular as a way for landowners to diversify income opportunities, improve management of forest resources, and increase biological diversity. People have been informally "farming the forests" for generations. However, in recent years, attention has been directed at formalizing forest farming and improving it...

  7. Jasmonates induce both defense responses and communication in monocotyledonous and dicotyledonous plants.

    PubMed

    Okada, Kazunori; Abe, Hiroshi; Arimura, Gen-ichiro

    2015-01-01

    Jasmonic acid (JA) and its derivatives (jasmonates, JAs) are phytohormones with essential roles in plant defense against pathogenesis and herbivorous arthropods. Both the up- and down-regulation of defense responses are dependent on signaling pathways mediated by JAs as well as other stress hormones (e.g. salicylic acid), generally those involving the transcriptional and post-transcriptional regulation of transcription factors via protein modification and epigenetic regulation. In addition to the typical model plant Arabidopsis (a dicotyledon), advances in genetics research have made rice a model monocot in which innovative pest control traits can be introduced and whose JA signaling pathway can be studied. In this review, we introduce the dynamic functions of JAs in plant defense strategy using defensive substances (e.g. indole alkaloids and terpenoid phytoalexins) and airborne signals (e.g. green leaf volatiles and volatile terpenes) in response to biotrophic and necrotrophic pathogens as well as above-ground and below-ground herbivores. We then discuss the important issue of how the mutualism of herbivorous arthropods with viruses or bacteria can cause cross-talk between JA and other phytohormones to counter the defense systems. © The Author 2014. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oup.com.

  8. Fuel From Farms: A Guide to Small-Scale Ethanol Production.

    ERIC Educational Resources Information Center

    Solar Energy Research Inst., Golden, CO.

    Ethanol and blends of ethanol and gasoline (such as gasohol) offer a near-term fuel alternative to oil. The focus of this handbook is upon the small-scale production of ethanol using farm crops as the source of raw materials. Provided are chapters on ethanol production procedures, feedstocks, plant design, and financial planning. Also presented…

  9. Multiple Reaction Monitoring Mode Based Liquid Chromatography-Mass Spectrometry Method for Simultaneous Quantification of Brassinolide and Other Plant Hormones Involved in Abiotic Stresses.

    PubMed

    Kasote, Deepak M; Ghosh, Ritesh; Chung, Jun Young; Kim, Jonggeun; Bae, Inhwan; Bae, Hanhong

    2016-01-01

    Plant hormones are the key regulators of adaptive stress response. Abiotic stresses such as drought and salt are known to affect the growth and productivity of plants. It is well known that the levels of plant hormones such as zeatin (ZA), abscisic acid (ABA), salicylic acid (SA), jasmonic acid (JA), and brassinolide (BR) fluctuate upon abiotic stress exposure. At present, there is not any single suitable liquid chromatography-mass spectrometry (LC-MS) method for simultaneous analysis of BR and other plant hormones involved in abiotic stresses. In the present study, we developed a simple, sensitive, and rapid method for simultaneous analysis of five major plant hormones, ZA, ABA, JA, SA, and BR, which are directly or indirectly involved in drought and salt stresses. The optimized extraction procedure was simple and easy to use for simultaneous measurement of these plant hormones in Arabidopsis thaliana. The developed method is highly reproducible and can be adapted for simultaneous measurement of changes in plant hormones (ZA, ABA, JA, SA, and BR) in response to abiotic stresses in plants like A. thaliana and tomato.

  10. Chemical composition and antifungal activity of plant extracts traditionally used in organic and biodynamic farming.

    PubMed

    Andreu, Vanessa; Levert, Annabel; Amiot, Anaïs; Cousin, Anaïs; Aveline, Nicolas; Bertrand, Cédric

    2018-03-07

    Five plant extracts traditionally used in organic and biodynamic farming for pest control and antifungal (downy mildew) disease management were selected after a farmer survey and analyzed for their chemical composition in LC-PDA-MS-MS and using adapted analytical method from food chemistry for determination of class of component (e.g., protein, sugar, lipids…). Their antifungal activity against Penicillium expansum, Botrytis cinerea, Botrytis allii, brown rot causing agents (Monilinia laxa and Monilinia fructigena), and grape downy mildew (Plasmopara viticola) was examined in vitro. White willow (Salix alba) and absinthe (Artemisia absinthium) ethanolic extracts were found to be the most effective in particular against Plasmopara viticola, with a total inhibition of spores germination when applied at 1000 mg/L. These extracts also showed a relatively low toxicity during preliminary ecotoxicological assays on Daphnia pulex. Extract from the bark of white willow contained some flavonoids, especially flavanones (eriodyctiol and derivates) and flavanols (catechins and derivates), as major compounds, whereas absinthe extract was rich in O-methylated flavanols and hydroxycinnamic acids. Thujone content in this extract was also determined by external calibration in GC-MS analysis, and its value was 0.004% dry extract.

  11. 26 CFR 1.1301-1 - Averaging of farm and fishing income.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ..., mollusks, crustaceans, and all other forms of marine animal and plant life, other than marine mammals and... averaging election). (4) Deposits into Merchant Marine Capital Construction Fund—(i) Reductions to taxable income and electible farm income. Under section 7518(c)(1)(A), certain deposits to a Merchant Marine...

  12. 26 CFR 1.1301-1 - Averaging of farm and fishing income.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ..., mollusks, crustaceans, and all other forms of marine animal and plant life, other than marine mammals and... averaging election). (4) Deposits into Merchant Marine Capital Construction Fund—(i) Reductions to taxable income and electible farm income. Under section 7518(c)(1)(A), certain deposits to a Merchant Marine...

  13. 26 CFR 1.1301-1 - Averaging of farm and fishing income.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ..., mollusks, crustaceans, and all other forms of marine animal and plant life, other than marine mammals and... averaging election). (4) Deposits into Merchant Marine Capital Construction Fund—(i) Reductions to taxable income and electible farm income. Under section 7518(c)(1)(A), certain deposits to a Merchant Marine...

  14. 26 CFR 1.1301-1 - Averaging of farm and fishing income.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ..., mollusks, crustaceans, and all other forms of marine animal and plant life, other than marine mammals and... averaging election). (4) Deposits into Merchant Marine Capital Construction Fund—(i) Reductions to taxable income and electible farm income. Under section 7518(c)(1)(A), certain deposits to a Merchant Marine...

  15. 7 CFR 1412.47 - Planting flexibility.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... payments will not be reduced for the planting or harvesting of the fruit, vegetable, or wild rice; (2) The... the payment acres for the farm will be reduced on an acre-for-acre basis; or (3) The producer has a...; and (ii) The payment acres for the farm will be reduced on an acre-for-acre basis. (e) Double-cropping...

  16. Fungal Diversity in Tomato Rhizosphere Soil under Conventional and Desert Farming Systems

    PubMed Central

    Kazerooni, Elham A.; Maharachchikumbura, Sajeewa S. N.; Rethinasamy, Velazhahan; Al-Mahrouqi, Hamed; Al-Sadi, Abdullah M.

    2017-01-01

    This study examined fungal diversity and composition in conventional (CM) and desert farming (DE) systems in Oman. Fungal diversity in the rhizosphere of tomato was assessed using 454-pyrosequencing and culture-based techniques. Both techniques produced variable results in terms of fungal diversity, with 25% of the fungal classes shared between the two techniques. In addition, pyrosequencing recovered more taxa compared to direct plating. These findings could be attributed to the ability of pyrosequencing to recover taxa that cannot grow or are slow growing on culture media. Both techniques showed that fungal diversity in the conventional farm was comparable to that in the desert farm. However, the composition of fungal classes and taxa in the two farming systems were different. Pyrosequencing revealed that Microsporidetes and Dothideomycetes are the two most common fungal classes in CM and DE, respectively. However, the culture-based technique revealed that Eurotiomycetes was the most abundant class in both farming systems and some classes, such as Microsporidetes, were not detected by the culture-based technique. Although some plant pathogens (e.g., Pythium or Fusarium) were detected in the rhizosphere of tomato, the majority of fungal species in the rhizosphere of tomato were saprophytes. Our study shows that the cultivation system may have an impact on fungal diversity. The factors which affected fungal diversity in both farms are discussed. PMID:28824590

  17. Exploring the Role of Farm Animals in Providing Care at Care Farms

    PubMed Central

    Hassink, Jan; De Bruin, Simone R.; Berget, Bente; Elings, Marjolein

    2017-01-01

    Simple Summary This paper provides insight into the role of farm animals in farm-based programs and their importance to different types of participants. Farm animals provide real work, close relationships, challenging tasks and opportunities for reflection. They also contribute to a welcoming atmosphere for various types of participants. Abstract We explore the role of farm animals in providing care to different types of participants at care farms (e.g., youngsters with behavioural problems, people with severe mental problems and people with dementia). Care farms provide alternative and promising settings where people can interact with animals compared to a therapeutic healthcare setting. We performed a literature review, conducted focus group meetings and carried out secondary data-analysis of qualitative studies involving care farmers and different types of participants. We found that farm animals are important to many participants and have a large number of potential benefits. They can (i) provide meaningful day occupation; (ii) generate valued relationships; (iii) help people master tasks; (iv) provide opportunities for reciprocity; (v) can distract people from them problems; (vi) provide relaxation; (vii) facilitate customized care; (viii) facilitate relationships with other people; (ix) stimulate healthy behavior; (x) contribute to a welcoming environment; (xi) make it possible to experience basic elements of life; and (xii) provide opportunities for reflection and feedback. This shows the multi-facetted importance of interacting with animals on care farms. In this study the types of activities with animals and their value to different types of participants varied. Farm animals are an important element of the care farm environment that can address the care needs of different types of participants. PMID:28574435

  18. Isotopic discrimination as a tool for organic farming certification in sweet pepper.

    PubMed

    del Amor, Francisco M; Navarro, Joaquín; Aparicio, Pedro M

    2008-01-01

    Organic farming is a form of agriculture that excludes the use of synthetic fertilizers, pesticides, and genetically modified organisms. These fertilizers have been traditionally overused in conventional farming to avoid lost revenue, but this often not does not take into account the potential contamination of aquifers and river due to nitrate leaching. Transition to organic farming practices could provide an instrument to reduce contamination and increase potential income. It is difficult to determine to what extent those fertilizers could have been used within a complete traceability of the production process. In this experiment, we evaluated the use of (15)N/(14)N isotopic discrimination in sweet pepper plants to test the hypothesis that synthetic fertilizers significantly reduce (15)N/(14)N compared with exclusively organic practices. Therefore, three common types of organic manures (sheep, hen, or horse) were applied at a rate of 8 kg m(-2) with or without synthetic fertilizer amendments under fully controlled environmental and irrigation conditions. Results indicate that (i) use of synthetic fertilizers significantly reduced (15/14)N(2)vsN(2)atm compared with treatments that only received water; (ii) with respect to the plant organs, old leaves and fruits were more sensitive to the synthetic fertilizer additions with reductions in (15/14)N(2)vsN(2)atm of 24.1 and 27.8%, respectively; and (iii) independently of the organic manure used, no additional fertilization (synthetic or organic) is required before 106 days after transplanting at that dosage because plant fresh weight was not reduced.

  19. uFarm: a smart farm management system based on RFID

    NASA Astrophysics Data System (ADS)

    Kim, Hyoungsuk; Lee, Moonsup; Jung, Jonghyuk; Lee, Hyunwook; Kim, Taehyoun

    2007-12-01

    Recently, the livestock industry in Korea has been threatened by many challenges such as low productivity due to labor intensiveness, global competition compelled by the Free Trade Agreement (FTA), and emerging animal disease issues such as BSE or foot-and-mouth. In this paper, we propose a smart farm management system, called uFarm, which would come up with such challenges by automating farm management. First, we automate labor-intensive jobs using equipments based on sensors and actuators. The automation subsystem can be controlled by remote user through wireless network. Second, we provide real-time traceability of information on farm animals using the radio-frequency identification (RFID) method and embedded data server with network connectivity.

  20. Deep Sequencing Reveals the Effect of MeJA on Scutellarin Biosynthesis in Erigeron breviscapus

    PubMed Central

    Xiao, Ying; Zhang, Feng; Chen, Jun-feng; Ji, Qian; Tan, He-Xin; Huang, Xin; Feng, Hao; Huang, Bao-Kang; Chen, Wan-Sheng; Zhang, Lei

    2015-01-01

    Background Erigeron breviscapus, a well-known traditional Chinese medicinal herb, is broadly used in the treatment of cerebrovascular disease. Scutellarin, a kind of flavonoids, is considered as the material base of the pharmaceutical activities in E. breviscapus. The stable and high content of scutellarin is critical for the quality and efficiency of E. breviscapus in the clinical use. Therefore, understanding the molecular mechanism of scutellarin biosynthesis is crucial for metabolic engineering to increase the content of the active compound. However, there is virtually no study available yet concerning the genetic research of scutellarin biosynthesis in E. breviscapus. Results Using Illumina sequencing technology, we obtained over three billion bases of high-quality sequence data and conducted de novo assembly and annotation without prior genome information. A total of 182,527 unigenes (mean length = 738 bp) were found. 63,059 unigenes were functionally annotated with a cut-off E-value of 10−5. Next, a total of 238 (200 up-regulated and 38 down-regulated genes) and 513 (375 up-regulated and 138 down-regulated genes) differentially expressed genes were identified at different time points after methyl jasmonate (MeJA) treatment, which fell into categories of ‘metabolic process’ and ‘cellular process’ using GO database, suggesting that MeJA-induced activities of signal pathway in plant mainly led to re-programming of metabolism and cell activity. In addition, 13 predicted genes that might participate in the metabolism of flavonoids were found by two co-expression analyses in E. breviscapus. Conclusions Our study is the first to provide a transcriptome sequence resource for E. breviscapus plants after MeJA treatment and it reveals transcriptome re-programming upon elicitation. As the result, several putative unknown genes involved in the metabolism of flavonoids were predicted. These data provide a valuable resource for the genetic and genomic studies of

  1. 50 CFR 14.23 - Live farm-raised fish and farm-raised fish eggs.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... eggs. 14.23 Section 14.23 Wildlife and Fisheries UNITED STATES FISH AND WILDLIFE SERVICE, DEPARTMENT OF... Exportation at Designated Ports § 14.23 Live farm-raised fish and farm-raised fish eggs. Live farm-raised fish and farm-raised fish eggs meet the definition of “bred in captivity” as stated in 50 CFR 17.3. Except...

  2. Long-chain omega-3 from low-trophic-level fish provides value to farmed seafood

    PubMed Central

    Bibus, Douglas M

    2015-01-01

    Low-trophic-level fish are a crucial source of long-chain (LC) omega-3 fatty acids for farmed fish and humans. Many farm-raised fish species have a clear need for these nutrients. Farmed fish deposit the LC omega-3s in their flesh and transfer them up the food chain. However, the content of LC omega-3s in farm-raised seafood continues to decline, while the content of shorter-chain plant-sourced omega-3s, and pro-inflammtory omega-6s continue to increase. This reduces its nutritional worth. The value of low-trophic-level fish is often viewed merely as its price at the dock. Some reports and metrics steer public attention towards the mass balance between quantities of low-trophic-level fish and farmed seafood. However, the the nutritional value of seafood is more important than its mere quantities. The role of low-trophic-level fish in human nutrition, health, and wellbeing is a fundamental component of its economic value to society. PMID:26097289

  3. Perception of volatiles produced by UVC-irradiated plants alters the response to viral infection in naïve neighboring plants.

    PubMed

    Yao, Youli; Danna, Cristian H; Ausubel, Frederick M; Kovalchuk, Igor

    2012-07-01

    Interplant communication of stress via volatile signals is a well-known phenomenon. It has been shown that plants undergoing stress caused by pathogenic bacteria or insects generate volatile signals that elicit defense response in neighboring naïve plants. Similarly, we have recently shown that naïve plants sharing the same gaseous environment with UVC-exposed plants exhibit similar changes in genome instability as UVC-exposed plants. We found that methyl salicylate (MeSA) and methyl jasmonate (MeJA) serve as volatile signals communicating genome instability (as measured by an increase in the homologous recombination frequency). UVC-exposed plants produce high levels of MeSA and MeJA, a response that is missing in an npr1 mutant. Concomitantly, npr1 mutants are impaired in communicating the signal leading to genome instability, presumably because this mutant does not develop new necrotic lesion after UVC irradiation as observed in wt plants. To analyze the potential biological significance of such plant-plant communication, we have now determined whether bystander plants that receive volatile signals from UVC-irradiated plants, become more resistant to UVC irradiation or infection with oilseed rape mosaic virus (ORMV). Specifically, we analyzed the number of UVC-elicited necrotic lesions, the level of anthocyanin pigments, and the mRNA levels corresponding to ORMV coat protein and the NPR1-regulated pathogenesis-related protein PR1 in the irradiated or virus-infected bystander plants that have been previously exposed to volatiles produced by UVC-irradiated plants. These experiments showed that the bystander plants responded similarly to control plants following UVC irradiation. Interestingly, however, the bystander plants appeared to be more susceptible to ORMV infection, even though PR1 mRNA levels in systemic tissue were significantly higher than in the control plants, which indicates that bystander plants could be primed to strongly respond to bacterial

  4. Whole farm quantification of GHG emissions within smallholder farms in developing countries

    NASA Astrophysics Data System (ADS)

    Seebauer, Matthias

    2014-03-01

    The IPCC has compiled the best available scientific methods into published guidelines for estimating greenhouse gas emissions and emission removals from the land-use sector. In order to evaluate existing GHG quantification tools to comprehensively quantify GHG emissions and removals in smallholder conditions, farm scale quantification was tested with farm data from Western Kenya. After conducting a cluster analysis to identify different farm typologies GHG quantification was exercised using the VCS SALM methodology complemented with IPCC livestock emission factors and the cool farm tool. The emission profiles of four farm clusters representing the baseline conditions in the year 2009 are compared with 2011 where farmers adopted sustainable land management practices (SALM). The results demonstrate the variation in both the magnitude of the estimated GHG emissions per ha between different smallholder farm typologies and the emissions estimated by applying two different accounting tools. The farm scale quantification further shows that the adoption of SALM has a significant impact on emission reduction and removals and the mitigation benefits range between 4 and 6.5 tCO2 ha-1 yr-1 with significantly different mitigation benefits depending on typologies of the crop-livestock systems, their different agricultural practices, as well as adoption rates of improved practices. However, the inherent uncertainty related to the emission factors applied by accounting tools has substantial implications for reported agricultural emissions. With regard to uncertainty related to activity data, the assessment confirms the high variability within different farm types as well as between different parameters surveyed to comprehensively quantify GHG emissions within smallholder farms.

  5. Pollution characteristics and environmental risk assessment of typical veterinary antibiotics in livestock farms in Southeastern China.

    PubMed

    Wang, Na; Guo, Xinyan; Xu, Jing; Kong, Xiangji; Gao, Shixiang; Shan, Zhengjun

    2014-01-01

    Scientific interest in pollution from antibiotics in animal husbandry has increased during recent years. However, there have been few studies on the vertical exposure characteristics of typical veterinary antibiotics in different exposure matrices from different livestock farms. This study explores the distribution and migration of antibiotics from feed to manure, from manure to soil, and from soil to vegetables, by investigating the exposure level of typical antibiotics in feed, manure, soil, vegetables, water, fish, and pork in livestock farms. A screening environmental risk assessment was conducted to identify the hazardous potential of veterinary antibiotics from livestock farms in southeast China. The results show that adding antibiotics to drinking water as well as the excessive use of antibiotic feed additives may become the major source of antibiotics pollution in livestock farms. Physical and chemical properties significantly affect the distribution and migration of various antibiotics from manure to soil and from soil to plant. Simple migration models can predict the accumulation of antibiotics in soil and plants. The environmental risk assessment results show that more attention should be paid to the terrestrial eco-risk of sulfadiazine, sulfamethazine, sulfamethoxazole, tetracycline, oxytetracycline, chlorotetracycline, ciprofloxacin, and enrofloxacin, and to the aquatic eco-risk of chlorotetracycline, ciprofloxacin, and enrofloxacin. This is the first systematic analysis of the vertical pollution characteristics of typical veterinary antibiotics in livestock farms in southeast China. It also identifies the ecological and human health risk of veterinary antibiotics.

  6. Edible plants for oral delivery of biopharmaceuticals.

    PubMed

    Merlin, Matilde; Pezzotti, Mario; Avesani, Linda

    2017-01-01

    Molecular farming is the use of plants for the production of high value recombinant proteins. Over the last 25 years, molecular farming has achieved the inexpensive, scalable and safe production of pharmaceutical proteins using a range of strategies. One of the most promising approaches is the use of edible plant organs expressing biopharmaceuticals for direct oral delivery. This approach has proven to be efficacious in several clinical vaccination and tolerance induction trials as well as multiple preclinical studies for disease prevention. The production of oral biopharmaceuticals in edible plant tissues could revolutionize the pharmaceutical industry by reducing the cost of production systems based on fermentation, and also eliminating expensive downstream purification, cold storage and transportation costs. This review considers the unique features that make plants ideal as platforms for the oral delivery of protein-based therapeutics and describes recent developments in the production of plant derived biopharmaceuticals for oral administration. © 2016 The British Pharmacological Society.

  7. Off-Farm Labour Decision of Canadian Farm Operators: Urbanization Effects and Rural Labour Market Linkages

    ERIC Educational Resources Information Center

    Alasia, Alessandro; Weersink, Alfons; Bollman, Ray D.; Cranfield, John

    2009-01-01

    Understanding the factors affecting off-farm labour decisions of census-farm operators has significant implications for rural development and farm income support policy. We examine the off-farm labour decisions of Canadian farm operators using micro-level data from the 2001 Census of Agriculture combined with community level data from the 2001…

  8. Indirect interactions between arbuscular mycorrhizal fungi and Spodoptera exigua alter photosynthesis and plant endogenous hormones.

    PubMed

    He, Lei; Li, Changyou; Liu, Runjin

    2017-08-01

    Peanut (Arachis hypogaea Linn. cv: Luhua 11) and tomato (Lycopersicon esculentum Mill. cv: Zhongshu 4) were inoculated with arbuscular mycorrhizal fungi (AMF) Funneliformis mosseae BEG167 (Fm), Rhizophagus intraradices BEG141 (Ri), and Glomus versiforme Berch (Gv), and/or Spodoptera exigua (S. exigua) under greenhouse conditions. Results indicated that feeding by S. exigua had little influence on colonization of peanut plants by AMF, but improved colonization of tomato by Fm and Gv. Feeding by S. exigua had little influence on leaf net photosynthetic rate, transpiration rate, and stomatal conductance of nonmycorrhizal peanut plants but significantly improved net photosynthetic rate and transpiration rate of mycorrhizal plants of both hosts. AMF with or without S. exigua inoculation improved host plant photosynthetic characteristics, growth, and hormone status. Fm showed maximum beneficial effects, followed by Gv. The concentrations and ratios of phytohormones abscisic acid (ABA), indole-3-acetic acid (IAA), gibberellin (GA), zeatin riboside (ZR), and jasmonic acid (JA) in the leaves of the host plants were changed due to the interaction between AMF and S. exigua. Generally, AMF with or without S. exigua inoculation increased the concentrations of GA, ZR, and JA and the ratios of IAA/ABA, GA/ABA, ZR/ABA, and IAA + GA + ZR/ABA, while feeding by S. exigua on nonmycorrhizal plants showed the opposite effect. The concentration of JA in the leaves of peanut and tomato inoculated with Fm or Fm + S. exigua was 1.9 and 1.9 times and 2.5 and 2.7 times, respectively, greater than that of the controls inoculated with neither. There was a negative correlation between the JA concentration and the survival percentage of S. exigua larva. We conclude that indirect interactions between AMF and insect herbivores changed the photosynthetic and hormone characteristics, and ratios of phytohormones, thereby revealing mechanisms of belowground-aboveground interactions.

  9. Farm Management Basic Core Curriculum. Kansas Postsecondary Farm and Ranch Management Project.

    ERIC Educational Resources Information Center

    Albracht, James, Ed.

    Thirty-five units of instruction are included in this core curriculum in farm management for postsecondary farm and ranch management programs. Units of instruction are divided into 12 instructional areas: (1) Introduction to Financial Management, (2) Farm Business Arrangement, (3) Credit Management, (4) Budgeting, (5) Recordkeeping, (6) Record…

  10. The Extent of Off-Farm Employment and Its Impact on Farm Women.

    ERIC Educational Resources Information Center

    Danes, Sharon M.; Keskinen, Susan M.

    1990-01-01

    Surveys off-farm employment of farm women and its impact on their lives. Describes survey data illustrating financial hardships experienced by working farm women. Describes imbalance between work and leisure as major source of dissatisfaction. Suggests service providers recognize women's "role overload" and its effects on families. (TES)

  11. Fodder Resource Uses and Assessment of Nitrogen Flows on Livestock Farming with Crop Production

    NASA Astrophysics Data System (ADS)

    Shirahase, Kyoko; Kobayashi, Hisashi

    With understanding the livestock farming on cattle breeding practiced increasing of self-production of fodders by the farmland's operation as “Livestock Farming with crop production”, we investigated the utilizations of actual fodder resources and farmland for two selected different types of livestock farming systems: “Multiple Type” which practices cattle raising with fodder cultivation, and “Grazing Type” which practices grazing and fodder cultivation with similar feed self-sufficiency rates. We also prepared and compared material and nitrogen flow of both livestock farming systems. The amount of nitrogen flow is clearly different between the two types though feed self-sufficiency rates are at similar level. Moreover, we defined “Internal Nitrogen Rate (INR)” which indicates the rate of internal nitrogen use to total nitrogen use in cattle raising, “Internal Nitrogen Circulation Rate (NCR)” which indicates the ratio of nitrogen amount in internal circulation to the nitrogen amount introduced from outside, and Nitrogen Outflow Potential (Op), which is the balance of nitrogen amount between input to farmlands and uptake by plants, and analyzed the balance of the amounts of nitrogen flows in both livestock farming type. It is suggested that “Grazing type”, which had the values of relatively high NCR and absolutely low Op, was the livestock farming type with high rates of nitrogen procurement from the interregional farming and low risk of nitrogen outflow.

  12. Presence of Stenotrophomonas maltophilia exhibiting high genetic similarity to clinical isolates in final effluents of pig farm wastewater treatment plants.

    PubMed

    Kim, Young-Ji; Park, Jin-Hyeong; Seo, Kun-Ho

    2018-03-01

    Although the prevalence of community-acquired Stenotrophomonas maltophilia infections is sharply increasing, the sources and likely transmission routes of this bacterium are poorly understood. We studied the significance of the presence of S. maltophilia in final effluents and receiving rivers of pig farm wastewater treatment plants (WWTPs). The loads and antibiotic resistance profiles of S. maltophilia in final effluents were assessed. Antibiotic resistance determinants and biofilm formation genes were detected by PCR, and genetic similarity to clinical isolates was investigated using multilocus sequence typing (MLST). S. maltophilia was recovered from final effluents at two of three farms and one corresponding receiving river. Tests of resistance to antibiotics recommended for S. maltophilia infection revealed that for each agent, at least one isolate was classified as resistant or intermediate, with the exception of minocycline. Furthermore, multidrug resistant S. maltophilia susceptible to antibiotics of only two categories was isolated and found to carry the sul2 gene, conferring trimethoprim/sulfamethoxazole resistance. All isolates carried spgM, encoding a major factor in biofilm formation. MLST revealed that isolates of the same sequence type (ST; ST189) were present in both effluent and receiving river samples, and phylogenetic analysis showed that all of the STs identified in this study clustered with clinical isolates. Moreover, one isolate (ST192) recovered in this investigation demonstrated 99.61% sequence identity with a clinical isolate (ST98) associated with a fatal infection in South Korea. Thus, the pathogenicity of the isolates reported here is likely similar to that of those from clinical environments, and WWTPs may play a role as a source of S. maltophilia from which this bacterium spreads to human communities. To the best of our knowledge, this represents the first report of S. maltophilia in pig farm WWTPs. Our results indicate that

  13. Farm Mapping to Assist, Protect, and Prepare Emergency Responders: Farm MAPPER.

    PubMed

    Reyes, Iris; Rollins, Tami; Mahnke, Andrea; Kadolph, Christopher; Minor, Gerald; Keifer, Matthew

    2014-01-01

    Responders such as firefighters and emergency medical technicians who respond to farm emergencies often face complex and unknown environments. They may encounter hazards such as fuels, solvents, pesticides, caustics, and exploding gas storage cylinders. Responders may be unaware of dirt roads within the farm that can expedite their arrival at critical sites or snow-covered manure pits that act as hidden hazards. A response to a farm, unless guided by someone familiar with the operation, may present a risk to responders and post a challenge in locating the victim. This project explored the use of a Web-based farm-mapping application optimized for tablets and accessible via easily accessible on-site matrix barcodes, or quick response codes (QR codes), to provide emergency responders with hazard and resource information to agricultural operations. Secured portals were developed for both farmers and responders, allowing both parties to populate and customize farm maps with icons. Data were stored online and linked to QR codes attached to mailbox posts where emergency responders may read them with a mobile device. Mock responses were conducted on dairy farms to test QR code linking efficacy, Web site security, and field usability. Findings from farmer usability tests showed willingness to enter data as well as ease of Web site navigation and data entry even with farmers who had limited computer knowledge. Usability tests with emergency responders showed ease of QR code connectivity to the farm maps and ease of Web site navigation. Further research is needed to improve data security as well as assess the program's applicability to nonfarm environments and integration with existing emergency response systems. The next phases of this project will expand the program for regional and national use, develop QR code-linked, Web-based extrication guidance for farm machinery for victim entrapment rescue, and create QR code-linked online training videos and materials for limited

  14. Farm Population of the United States: 1976. Current Population Reports: Farm Population.

    ERIC Educational Resources Information Center

    Banks, Vera J.; And Others

    Prepared cooperatively by the Bureau of the Census and the Economic Research Service of the U.S. DeparLment of Agriculture, this document presents narrative and tabular data on: demographic and social characteristics of the farm population; economic characteristics of the farm population; revision of farm population processing procedures; and…

  15. Kd Values for Agricultural and Surface Soils for Use in Hanford Site Farm, Residential, and River Shoreline Scenarios

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Serne, R. Jeffrey

    This report provides best estimate Kd values and a minimum and maximum range of Kd values to be used for agricultural soils and Columbia River bank sediments that exist today or would exist in the future when portions of the Hanford Site are released for farming, residential, and recreational use after the U. S. Department of Energy (DOE) completes clean up of defense waste on the site. The Kd values should be used to determine the fate and transport rates of contaminants and their availability for plant and animal uptake in selected non-groundwater scenarios included in Hanford Site environmental impactmore » statements, risk assessments and specific facility performance assessments. This report describes scenarios such as a small farm where drilling of a well inadvertently goes through buried waste and brings waste to the surface, allowing the tailings to become available for direct human exposure or incorporation into garden crops and farm animals used for food by the farm family. The Kd values recommended in this report can also be used to calculate sediment-water partitioning factors used to predict plant and animal uptake from interaction with the contaminated soil.« less

  16. Phytoremediation Capabilities of Spirodela polyrhiza and Salvinia molesta in Fish Farm Wastewater: A Preliminary Study

    NASA Astrophysics Data System (ADS)

    Ng, Y. S.; Samsudin, N. I. S.; Chan, D. J. C.

    2017-06-01

    Fish farm wastewater needs to be treated as it contains considerably high loading of suspended solids and dissolved nutrients from accumulation of by-products e.g. fish excretions and uneaten feed. In this study, macrophytes, namely Spirodela polyrhiza and Salvinia molesta were examined for their phytoremediation efficiency in treating fish farm wastewater in a raceway pond rig. It was carried out indoor for 14 days under controlled environment. Water samples was collected once every 2 days for analysis of NO3 -- N, PO4 3-, NH3-N, COD, turbidity, MLVSS and pH. The results showed that there was decrement of phosphate in fish farm wastewater using either S. polyrhiza or S. molesta. Interestingly, S. polyrhiza was found to be more efficient in phosphate uptake as it removed 72% phosphate at day 4 and up to 95% in the end of the experiment whereas 72% phosphate removal was only achieved by S. molesta at day 10. Similar ammonia decrement was observed for both plants and most of the ammonia were not detected in the wastewater by day 10 for S. polyrhiza, while by day 8 for S. molesta. Nitrate showed increment for both plants which could be due to nitrification. Both plants achieved highest COD removal on day 12, whereby 68% for S. polyrhiza and 63% for S. molesta. They were able to reduce turbidity and total suspended solids (TSS) to very low level and significantly increase clarity of wastewater. S. polyrhiza reduced up to 96% of initial turbidity value and 86% of TSS. 82% reduction of initial turbidity and 79% TSS decrement were observed for S. molesta. pH fluctuations were minimum for both plants, with a range between 7.62 to 7.77. Both plants demonstrated biomass increment for fresh weight in which 84% for S. polyrhiza while 85% for S. molesta. This study proved that the macrophytes were able to treat fish farm wastewater by significantly removing phosphate, ammonia, turbidity and TSS. It aids in minimizing pollutants released to receiving waters and producing biomass

  17. Improving Environmental Management on Small-scale Farms: Perspectives of Extension Educators and Horse Farm Operators

    NASA Astrophysics Data System (ADS)

    Rebecca, Perry-Hill; Linda, Prokopy

    2015-01-01

    Although the number of small-scale farms is increasing in North America and Europe, few studies have been conducted to better understand environmental management in this sector. We investigate this issue by examining environmental management on horse farms from both the perspective of the "expert" extension educator and horse farm operator. We conducted a Delphi survey and follow-up interviews with extension educators in Indiana and Kentucky. We also conducted interviews and farm assessments with 15 horse farm operators in the two states. Our results suggest a disconnection between the perceptions of extension educators and horse farm operators. Extension educators believed that operators of small horse farms are unfamiliar with conservation practices and their environmental benefits and they found it difficult to target outreach to this audience. In the interviews with horse farm operators, we found that the majority were somewhat familiar with conservation practices like rotational grazing, soil testing, heavy use area protection, and manure composting. It was not common, however, for practices to be implemented to generally recognized standards. The horse farm respondents perceived these practices as interrelated parts of a system of farm management that has developed over time to best deal with the physical features of the property, needs of the horses, and available resources. Because conservation practices must be incorporated into a complex farm management system, traditional models of extension (i.e., diffusion of innovations) may be inappropriate for promoting better environmental management on horse farms.

  18. Improving environmental management on small-scale farms: perspectives of extension educators and horse farm operators.

    PubMed

    Rebecca, Perry-Hill; Linda, Prokopy

    2015-01-01

    Although the number of small-scale farms is increasing in North America and Europe, few studies have been conducted to better understand environmental management in this sector. We investigate this issue by examining environmental management on horse farms from both the perspective of the "expert" extension educator and horse farm operator. We conducted a Delphi survey and follow-up interviews with extension educators in Indiana and Kentucky. We also conducted interviews and farm assessments with 15 horse farm operators in the two states. Our results suggest a disconnection between the perceptions of extension educators and horse farm operators. Extension educators believed that operators of small horse farms are unfamiliar with conservation practices and their environmental benefits and they found it difficult to target outreach to this audience. In the interviews with horse farm operators, we found that the majority were somewhat familiar with conservation practices like rotational grazing, soil testing, heavy use area protection, and manure composting. It was not common, however, for practices to be implemented to generally recognized standards. The horse farm respondents perceived these practices as interrelated parts of a system of farm management that has developed over time to best deal with the physical features of the property, needs of the horses, and available resources. Because conservation practices must be incorporated into a complex farm management system, traditional models of extension (i.e., diffusion of innovations) may be inappropriate for promoting better environmental management on horse farms.

  19. The field efficacy of garlic extract against Dermanyssus gallinae in layer farms of Babol, Iran.

    PubMed

    Faghihzadeh Gorji, Shohreh; Faghihzadeh Gorji, Sina; Rajabloo, Mohammad

    2014-03-01

    Dermanyssus gallinae (Acari: Mesostigmata) is the most important hematophagous ectoparasite in layer farms in many countries. The reproduction rate of the parasite is rapid and can be completed in a week under favorable conditions. The parasite has direct and indirect effects on birds. It can also act as a vector for some important pathogens. Many researchers have investigated the effects of essential oils, plant extracts, oriental medicinal plant extracts, and silica against red mite. They can be used as killing agents or repellents. In the present study, the effect of garlic (Allium sativum) extract was investigated for controlling red mite infestation in a layer farm in Babol, North of Iran. Our results showed that the extract was effective and we obtained a 96% success after two successive sprays.

  20. Farm Household Survival Strategies and Diversification on Marginal Farms

    ERIC Educational Resources Information Center

    Meert, H.; Van Huylenbroeck, G.; Vernimmen, T.; Bourgeois, M.; van Hecke, E.

    2005-01-01

    On marginal farms, and in agriculture in general, sustainability is largely guaranteed by a broad range of survival strategies, closely interlinked and embedded in the household structure of typical family farms. This paper reports results of a socio-economic study carried out among Belgian farmers, focusing specifically on the opportunities…

  1. Climate change vulnerability, adaptation and risk perceptions at farm level in Punjab, Pakistan.

    PubMed

    Abid, Muhammad; Schilling, Janpeter; Scheffran, Jürgen; Zulfiqar, Farhad

    2016-03-15

    Pakistan is among the countries highly exposed and vulnerable to climate change. The country has experienced many severe floods, droughts and storms over the last decades. However, little research has focused on the investigation of vulnerability and adaptation to climate-related risks in Pakistan. Against this backdrop, this article investigates the farm level risk perceptions and different aspects of vulnerability to climate change including sensitivity and adaptive capacity at farm level in Pakistan. We interviewed a total of 450 farming households through structured questionnaires in three districts of Punjab province of Pakistan. This study identified a number of climate-related risks perceived by farm households such as extreme temperature events, insect attacks, animal diseases and crop pests. Limited water availability, high levels of poverty and a weak role of local government in providing proper infrastructure were the factors that make farmers more sensitive to climate-related risks. Uncertainty or reduction in crop and livestock yields; changed cropping calendars and water shortage were the major adverse impacts of climate-related risks reported by farmers in the study districts. Better crop production was reported as the only positive effect. Further, this study identified a number of farm level adaptation methods employed by farm households that include changes in crop variety, crop types, planting dates and input mix, depending upon the nature of the climate-related risks. Lack of resources, limited information, lack of finances and institutional support were some constraints that limit the adaptive capacity of farm households. This study also reveals a positive role of cooperation and negative role of conflict in the adaptation process. The study suggests to address the constraints to adaptation and to improve farm level cooperation through extended outreach and distribution of institutional services, particularly climate-specific farm advisory

  2. The effects of pre-slaughter pig management from the farm to the processing plant on pork quality.

    PubMed

    Edwards, L N; Grandin, T; Engle, T E; Ritter, M J; Sosnicki, A A; Carlson, B A; Anderson, D B

    2010-12-01

    Two experiments (Exp.1, n=80; Exp.2, n=144) were conducted to determine the effects of pre-slaughter pig management on pork quality by monitoring blood lactate concentration ([LAC]) during marketing. [LAC] was measured at: (1) baseline at farm, (2) post-loading on truck, (3) pre-unloading after transport, (4) post-unloading at plant, (5) post-lairage, (6) post-movement to stun, and (7) exsanguination. Pearson correlations were used to determine relationships between [LAC] and meat quality. Higher [LAC] post-loading or a greater change in [LAC] during loading resulted in increased 24h pH (P=0.002, P=0.0006, Exp.1; P=0.0001, P=0.01, Exp.2, respectively), decreased L* (P=0.03, P=0.04; P=0.001, P=0.01) and decreased drip loss (P=0.02, P=0.12; P=0.002, P=0.01). Even though improved handling during loading is important to animal well-being, it will not necessarily translate into improved pork quality. Copyright © 2010 The American Meat Science Association. Published by Elsevier Ltd. All rights reserved.

  3. Plants on constant alert: elevated levels of jasmonic acid and jasmonate-induced transcripts in caterpillar resistant maize

    USDA-ARS?s Scientific Manuscript database

    Plant defense responses against insect herbivores frequently depend on the biosynthesis and action of jasmonic acid (JA) and its conjugates. To better understand JA signaling pathways in maize (Zea mays L.), we have examined two maize genotypes, Mp708 and Tx601. Mp708 is resistant to feeding by le...

  4. Farming strategies to feed people, facilitate essential soil services, and fuel the economy

    USDA-ARS?s Scientific Manuscript database

    Perennial cellulosic biomass and food crop residues are important on-farm resources, which have become potential valuable sources of income as a harvestable commodity contributing to biofuel production demands. Inputs of carbon embedded in above-ground plant biomass are a key biological energy sourc...

  5. Neither "Family" nor "Corporate" Farming: Australian Tomato Growers as Farm Family Entrepreneurs

    ERIC Educational Resources Information Center

    Pritchard, Bill; Burch, David; Lawrence, Geoffrey

    2007-01-01

    For the past two decades there has been much debate about the future of family farming. The basic question on which this debate has turned is whether current pressures on family farm systems should be understood as symptomatic of a terminal condition, in which farmers are replaced progressively by corporate ownership; or whether family farms will…

  6. Farm Population of the United States: 1971. Current Population Reports: Farm Population.

    ERIC Educational Resources Information Center

    Bureau of the Census (DOC), Suitland, MD. Population Div.

    Based on data derived from the Current Population Survey of the Bureau of the Census, this statistical report presents demographic and labor force characteristics of the U.S. farm population and comparisons of the farm and nonfarm populations. Tabular data are presented as follows: (1) U.S. Population, Total and Farm: April 1960 and 1971; (2)…

  7. Farm Population of the United States: 1972. Current Population Reports: Farm Population.

    ERIC Educational Resources Information Center

    Bureau of the Census (DOC), Suitland, MD. Population Div.

    Based on data derived from the Current Population Survey of the Bureau of Census, this statistical report presents demographic and labor force characteristics of the U.S. farm population and comparisons of the farm and nonfarm populations. Tabular data are presented as follows: (1) U.S. Population, Total and Farm: April 1960 to 1972; (2) Persons…

  8. [Host plants of Aphis gossypii (Aphididae), vector of virus of Cucumis melo melon (Cucurbitaceae) in Costa Rica].

    PubMed

    Sánchez, M V; Agüero, R; Rivera, C

    2001-03-01

    Plant species associated with commercial melon crops and surrounding areas were examined to identity the natural host plants of Aphis gossypii Glover. The study was conducted in two farms located in different melon production areas and plant life zones of Costa Rica. Plant species diversity, percent coverage and distribution over time were recorded during one year. Differences between locations were observed. A total of 86 plant species (49 families) and 72 plant species (40 families) were identified associated to the crop in farms A and B, respectively. In both farms a total of 24 species plants (16 families) were colonized by A. gossypii and 16 (10 families) are new reports of host plant species for this aphid. The new reports are: Justicia comata, Tetramerium nervosum, Alternanthera pubiflora, Cassia massoni, C. reticulata, Cleome viscosa, C. spinosa, Croton argenteus, Caperonia palustris, Chamaesyce gyssopilopia, Phyllantus amarus, Sida decumbens, Ludwigia erecta, Passiflora foetida, Guazuma ulmifolia and Corchorus orinocensis.

  9. Verification of Egg Farming Systems from The Netherlands and New Zealand Using Stable Isotopes.

    PubMed

    Rogers, Karyne M; van Ruth, Saskia; Alewijn, Martin; Philips, Andy; Rogers, Pam

    2015-09-30

    Stable isotopes were used to develop authentication criteria of eggs laid under cage, barn, free range, and organic farming regimens from The Netherlands and New Zealand. A training set of commercial poultry feeds and egg albumen from 49 poultry farms across The Netherlands was used to determine the isotopic variability of organic and conventional feeds and to assess trophic effects of these corresponding feeds and barn, free range, and organic farming regimens on corresponding egg albumen. A further 52 brands of New Zealand eggs were sampled from supermarket shelves in 2008 (18), 2010 (30), and 2014 (4) to characterize and monitor changes in caged, barn, free range, and organic egg farming regimens. Stable carbon (δ(13)C) and nitrogen (δ(15)N) isotopes of 49 commercial poultry feeds and their corresponding egg albumens reveals that Dutch poultry are fed exclusively on a plant-based feed and that it is possible to discriminate between conventional and organic egg farming regimens in The Netherlands. Similarly, it is possible to discriminate between New Zealand organic and conventional egg farming regimens, although in the initial screening in 2008, results showed that some organic eggs had isotope values similar to those of conventional eggs, suggesting hens were not exclusively receiving an organic diet. Dutch and New Zealand egg regimens were shown to have a low isotopic correlation between both countries, because of different poultry feed compositions. In New Zealand, both conventional and organic egg whites have higher δ(15)N values than corresponding Dutch egg whites, due to the use of fishmeal or meat and bone meal (MBM), which is banned in European countries. This study suggests that stable isotopes (specifically nitrogen) show particular promise as a screening and authentication tool for organically farmed eggs. Criteria to assess truthfulness in labeling of organic eggs were developed, and we propose that Dutch organic egg whites should have a minimum

  10. Messages from the Other Side: Parasites Receive Damage Cues from their Host Plants.

    PubMed

    Tjiurutue, Muvari Connie; Stevenson, Philip C; Adler, Lynn S

    2016-08-01

    As sessile organisms, plants rely on their environment for cues indicating imminent herbivory. These cues can originate from tissues on the same plant or from different individuals. Since parasitic plants form vascular connections with their host, parasites have the potential to receive cues from hosts that allow them to adjust defenses against future herbivory. However, the role of plant communication between hosts and parasites for herbivore defense remains poorly investigated. Here, we examined the effects of damage to lupine hosts (Lupinus texensis) on responses of the attached hemiparasite (Castilleja indivisa), and indirectly, on a specialist herbivore of the parasite, buckeyes (Junonia coenia). Lupines produce alkaloids that act as defenses against herbivores that can be taken up by the parasite. We found that damage to lupine host plants by beet armyworm (Spodoptera exigua) significantly increased jasmonic acid (JA) levels in both the lupine host and parasite, suggesting uptake of phytohormones or priming of parasite defenses by using host cues. However, lupine host damage did not induce changes in alkaloid levels in the hosts or parasites. Interestingly, the parasite had substantially higher concentrations of JA and alkaloids compared to lupine host plants. Buckeye herbivores consumed more parasite tissue when attached to damaged compared to undamaged hosts. We hypothesize that increased JA due to lupine host damage induced higher iridoid glycosides in the parasite, which are feeding stimulants for this specialist herbivore. Our results demonstrate that damage to hosts may affect both parasites and associated herbivores, indicating cascading effects of host damage on multiple trophic levels.

  11. How Do Plants and Animals Prepare for Winter?

    ERIC Educational Resources Information Center

    Larm, Brooke

    2017-01-01

    This article describes how a farm-based class in the Great Lakes region investigated how plants and animals prepare for winter. Two groups of children, ranging in ages from three to five years old, had a farm, pasture, gardens, forest, and a pond available for exploration. A low teacher-to-child ratio was maintained, with one teacher to…

  12. Extrafloral nectar production of the ant-associated plant, Macaranga tanarius, is an induced, indirect, defensive response elicited by jasmonic acid

    PubMed Central

    Heil, Martin; Koch, Thomas; Hilpert, Andrea; Fiala, Brigitte; Boland, Wilhelm; Linsenmair, K. Eduard

    2001-01-01

    Plant species in at least 66 families produce extrafloral nectar (EFN) on their leaves or shoots and therewith attract predators and parasitoids, such as ants and wasps, which in turn defend them against herbivores. We investigated whether EFN secretion is induced by herbivory and/or artificial damage, and thus can be regarded as an induced defensive response. In addition, we studied the underlying signaling pathway. EFN secretion by field-grown Macaranga tanarius increased after herbivory, artificial leaf damage, and exogenous jasmonic acid (JA) application. Artificial damage strongly enhanced endogenous JA concentrations. The response in EFN production to artificial damage was much less pronounced in those leaves that were treated with phenidone to inhibit endogenous JA synthesis. Quantitative dose–response relations were found between the increase in nectar production and both the intensity of leaf damage and the amounts of exogenously applied JA. The amount of endogenously produced JA was positively correlated with the intensity of leaf damage. Increased numbers of defending insects and decreased numbers of herbivores were observed on leaves after inducing EFN production by exogenous JA treatment. Over 6 weeks, repeatedly applied JA or artificial damage resulted in a ten-fold reduction in herbivory. These results demonstrate that EFN production represents an alternative mechanism for induced, indirect defensive plant responses that are mediated via the octadecanoid signal transduction cascade. PMID:11158598

  13. Upscaling Arbuscular Mycorrhizal Symbiosis and Related Agroecosystems Services in Smallholder Farming Systems

    PubMed Central

    Oruru, Marjorie Bonareri; Njeru, Ezekiel Mugendi

    2016-01-01

    Smallholder farming systems form unique ecosystems that can protect beneficial soil biota and form an important source of useful genetic resources. They are characterized by high level of agricultural diversity mainly focused on meeting farmers' needs. Unfortunately, these systems often experience poor crop production mainly associated with poor planning and resource scarcity. Soil fertility is among the primary challenges faced by smallholder farmers, which necessitate the need to come up with affordable and innovative ways of replenishing soils. One such way is the use of microbial symbionts such as arbuscular mycorrhizal fungi (AMF), a beneficial group of soil microbiota that form symbiotic associations with majority of cultivated crops and play a vital role in biological soil fertility, plant nutrition, and protection. AMF can be incorporated in smallholder farming systems to help better exploit chemical fertilizers inputs which are often unaffordable to many smallholder farmers. The present review highlights smallholder farming practices that could be innovatively redesigned to increase AMF symbiosis and related agroecosystem services. Indeed, the future of global food security depends on the success of smallholder farming systems, whose crop productivity depends on the services provided by well-functioning ecosystems, including soil fertility. PMID:26942194

  14. Health effects of agrochemicals among farm workers in commercial farms of Kwekwe district, Zimbabwe

    PubMed Central

    Magauzi, Regis; Mabaera, Bigboy; Rusakaniko, Simbarashe; Chimusoro, Anderson; Ndlovu, Nqobile; Tshimanga, Mufuta; Shambira, Gerald; Chadambuka, Addmore; Gombe, Notion

    2011-01-01

    Introduction Farm workers are at a very high risk of occupational diseases due to exposure to pesticides resulting from inadequate education, training and safety systems. The farm worker spends a lot of time exposed to these harmful agrochemicals. Numerous acute cases with symptoms typical of agrochemical exposure were reported from the commercial farms. We assessed the health effects of agrochemicals in farm workers in commercial farms of Kwekwe District (Zimbabwe), in 2006. Methods An analytical cross sectional study was conducted amongst a sample of 246 farm workers who handled agrochemicals when discharging their duties in the commercial farms. Plasma cholinesterase activity in blood specimens obtained from farm workers was measured using spectrophotometry to establish levels of poisoning by organophosphate and/or carbamates. Information on the knowledge, attitudes and practices of farm workers on agrochemicals use was collected using a pre-tested interviewer administered questionnaire. Bivariate and multivariate analyses were conducted to determine factors that were associated with abnormal cholinesterase activity. Results The prevalence of organophosphate poisoning, indicated by cholinesterase activity of 75% or less, was 24.1%. The median period of exposure to agrochemicals was 3 years (Q1:=1 year, Q3:=7 years). Ninety eight (41.5%) farm workers knew the triangle colour code for the most dangerous agrochemicals. Not being provided with personal protective equipment (OR 2.00; 95% CI: 1.07 – 3.68) and lack of knowledge of the triangle colour code for most dangerous agrochemicals (OR 2.02; 95% CI: 1.02 – 4.03) were significantly associated with abnormal cholinesterase activity. Conclusion There was organophosphate poisoning in the commercial farms. Factors that were significantly associated with the poisoning were lack of protective clothing and lack of knowledge of the triangle colour code for most dangerous agrochemicals. We recommended intensive health

  15. Health effects of agrochemicals among farm workers in commercial farms of Kwekwe district, Zimbabwe.

    PubMed

    Magauzi, Regis; Mabaera, Bigboy; Rusakaniko, Simbarashe; Chimusoro, Anderson; Ndlovu, Nqobile; Tshimanga, Mufuta; Shambira, Gerald; Chadambuka, Addmore; Gombe, Notion

    2011-01-01

    Farm workers are at a very high risk of occupational diseases due to exposure to pesticides resulting from inadequate education, training and safety systems. The farm worker spends a lot of time exposed to these harmful agrochemicals. Numerous acute cases with symptoms typical of agrochemical exposure were reported from the commercial farms. We assessed the health effects of agrochemicals in farm workers in commercial farms of Kwekwe District (Zimbabwe), in 2006. An analytical cross sectional study was conducted amongst a sample of 246 farm workers who handled agrochemicals when discharging their duties in the commercial farms. Plasma cholinesterase activity in blood specimens obtained from farm workers was measured using spectrophotometry to establish levels of poisoning by organophosphate and/or carbamates. Information on the knowledge, attitudes and practices of farm workers on agrochemicals use was collected using a pre-tested interviewer administered questionnaire. Bivariate and multivariate analyses were conducted to determine factors that were associated with abnormal cholinesterase activity. The prevalence of organophosphate poisoning, indicated by cholinesterase activity of 75% or less, was 24.1%. The median period of exposure to agrochemicals was 3 years (Q(1):=1 year, Q(3):=7 years). Ninety eight (41.5%) farm workers knew the triangle colour code for the most dangerous agrochemicals. Not being provided with personal protective equipment (OR 2.00; 95% CI: 1.07 - 3.68) and lack of knowledge of the triangle colour code for most dangerous agrochemicals (OR 2.02; 95% CI: 1.02 - 4.03) were significantly associated with abnormal cholinesterase activity. There was organophosphate poisoning in the commercial farms. Factors that were significantly associated with the poisoning were lack of protective clothing and lack of knowledge of the triangle colour code for most dangerous agrochemicals. We recommended intensive health education and training of farm workers on

  16. 26 CFR 1.1301-1T - Averaging of farm and fishing income (temporary).

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... finfish, mollusks, crustaceans, and all other forms of marine animal and plant life, other than marine... averaging election). (4) Deposits into Merchant Marine Capital Construction Fund—(i) Reductions to taxable income and electible farm income. Under section 7518(c)(1)(A), certain deposits to a Merchant Marine...

  17. RNA sequencing on Amomum villosum Lour. induced by MeJA identifies the genes of WRKY and terpene synthases involved in terpene biosynthesis.

    PubMed

    He, Xueying; Wang, Huan; Yang, Jinfen; Deng, Ke; Wang, Teng

    2018-02-01

    Amomum villosum Lour. is an important Chinese medicinal plant that has diverse medicinal functions, and mainly contains volatile terpenes. This study aims to explore the WRKY transcription factors (TFs) and terpene synthase (TPS) unigenes that might be involved in terpene biosynthesis in A. villosum, and thus providing some new information on the regulation of terpenes in plants. RNA sequencing of A. villosum induced by methyl jasmonate (MeJA) revealed that the WRKY family was the second largest TF family in the transcriptome. Thirty-six complete WRKY domain sequences were expressed in response to MeJA. Further, six WRKY unigenes were highly correlated with eight deduced TPS unigenes. Ultimately, we combined the terpene abundance with the expression of candidate WRKY TFs and TPS unigenes to presume a possible model wherein AvWRKY61, AvWRKY28, and AvWRKY40 might coordinately trans-activate the AvNeoD promoter. We propose an approach to further investigate TF unigenes that might be involved in terpenoid biosynthesis, and identified four unigenes for further analyses.

  18. Farm and cow-level prevalence of bovine digital dermatitis on dairy farms in Taranaki, New Zealand.

    PubMed

    Yang, D A; Heuer, C; Laven, R; Vink, W D; Chesterton, R N

    2017-09-01

    The aims of this cross-sectional study were to investigate the herd and cow-level prevalence of bovine digital dermatitis (BDD) in dairy farms in the northern Taranaki region of New Zealand, and to identify whether there was any spatial clustering of herds with the disease. A survey of 224 dairy farms in the northern Taranaki region of New Zealand was undertaken from September 2014 to February 2015. Following training in robust criteria to confirm BDD visually, a technician inspected the rear feet of every milking cow on the farms during milking. The identity of cows with lesions and the feet involved were recorded. The proportion of cows affected among the inspected population (cow-level prevalence), the proportion of a herd affected (farm-level prevalence), and proportion of farms with ≥1 cow with lesions, were calculated. A bivariate K function analysis was then used to assess whether farms with ≥1 cow with lesions were clustered, after accounting for the distribution of the farms involved in the study. Bovine digital dermatitis lesions were observed on 143/224 (63.8 (95% CI=57.5-70.1)%) farms. Within-farm prevalence was 0% on 81 (36.2%) farms, between >0 and <3% on 120 (53.5%) farms, with a maximum prevalence of 12.7% on one farm. Overall, cow-level prevalence was 707/60,455 (1.2 (95% CI=0.9-3.0)%), and on affected farms was 707/41,116 (1.7 (95% CI=1.4-2.1)%). In affected cows, 268/707 (37.9%) had a lesion on left foot only, 262/707 (37.1%) on the right foot only and 177/707 (25.0%) on both feet. The K function analysis showed no evidence of clustering of farms with BDD. Bovine digital dermatitis was widespread among the survey farms, but there was no evidence that there was any clustering of herds with BDD. The cow-level prevalence on affected farms was much lower than reported elsewhere. Although the prevalence at the cow level was low, if these data are representative of other regions of New Zealand, BDD could easily become a major problem on dairy farms

  19. The History of Recent Farm Legislation: Implications for Farm Families.

    ERIC Educational Resources Information Center

    Little, Linda F.; And Others

    1987-01-01

    Presents history of modern farm legislation and looks at recent legislation and tax policies. Asserts that family scientists attempting to help farm families can benefit from understanding legislation and policies. Discusses family intervention strategies in the larger context of macroeconomic and political forces. (Author/NB)

  20. Effect of long-term farming strategies on soil microbiota and soil health

    NASA Astrophysics Data System (ADS)

    Sommermann, Loreen; Babin, Doreen; Sandmann, Martin; Smalla, Kornelia; Schellenberg, Ingo; Grosch, Rita; Geistlinger, Joerg

    2017-04-01

    Increasing food and energy demands have resulted in considerable intensification of farming practices, which brought about severe consequences for agricultural soils, e.g. loss of fertility, erosion and enrichment of soil-borne plant diseases. In order to maintain soil quality and health for the future, the development of more extensive and sustainable farming strategies is urgently needed. The soil microbiome is regarded as a key player in soil ecosystem functions, particularly the natural ability of soils to suppress plant pathogens (suppressiveness). Recent studies showed that soil microbial communities are influenced by agricultural management. To further analyze the effects of farming strategies on soil suppressiveness and plant performance, agricultural soils from three long-term field trials in Thyrow, Bernburg (both in Germany) and Therwil (Switzerland) were sampled and subjected to molecular profiling of soil bacteria and fungi using marker genes and high-throughput amplicon sequencing. Significant effects on bacterial as well as fungal community composition, including plant pathogenic and beneficial taxa, were observed among variants of tillage and crop rotation. The least effect on both communities had fertilization, with no significance between variants. Subsequently, the same soils were subjected to growth chamber pot experiments with lettuce as a model (Lactuca sativa). After a growth period of six weeks significant differences in lettuce shoot and soil microbial biomass were observed among soil samples of the different long-term trials. Furthermore, the lettuce rhizosphere exhibited diverse bacterial community compositions as observed by DGGE (denaturing gradient gel electrophoresis). Using group-specific PCR-DGGE fingerprints, bacterial responders to fertilization, soil management and crop rotation were identified among different taxonomic groups. Currently, bacterial and fungal amplicon sequencing of rhizosphere and bulk soil from these pot

  1. Respirator Use Among US Farm Operators With Asthma: Results From the 2011 Farm and Ranch Safety Survey

    PubMed Central

    Casey, Megan L.; Mazurek, Jacek M.

    2017-01-01

    Objective The purpose of this study was to estimate the national prevalence of respirator use among farm operators with farm work–related asthma and factors associated with respirator use. Methods The authors examined the 2011 Farm and Ranch Safety Survey, a national survey collected from 11,210 actively farming farm operators in the United States. Adjusted prevalence ratios (aPORs) of respirator use were calculated by demographic characteristics, farm characteristics, asthma characteristics, and selected exposures and hazards. Results Among the estimated 2.2 million farm operators in 2011, 35.7% reported using a respirator in the past 12 months. Respirator use was significantly (P <.05) associated with age, marital status, sex, smoking status, farm value of sales, farm type, farm acreage, and geographic region. Operators who work with pesticides were 3.5 times more likely to use respirator than those who did not work with pesticides (P < .0001). Among those with current asthma, 60.8% of operators with farm work–related asthma used respirators compared with 44.4% of operators with non–farm work–related asthma (P = .03). Farm operators with farm work–related asthma who had an asthma attack at work were 11.3 times more likely to report respirator use than those who did not have an asthma attack at work (P = .03). Conclusions Personal protective equipment, including respirators, is an approach to reducing respiratory exposures in agricultural settings, in particular among those with farm work–related asthma. Education for respirator use and evaluation for respirator tolerance should be considered. PMID:28095135

  2. Farm Population of the United States: 1977. Current Population Reports: Farm Population.

    ERIC Educational Resources Information Center

    Banks, Vera J.; DeAre, Diana

    The farm population has declined fairly steadily for more than half a century. By 1970 the proportion of the U.S. population residing on farms had fallen to about 5 per cent, and by 1977 had dropped to 3.6 per cent. About 1.4 per cent of the farm population was of Spanish origin (represented for the first time in this year's report), as compared…

  3. The COP9 signalosome controls jasmonic acid synthesis and plant responses to herbivory and pathogens.

    PubMed

    Hind, Sarah R; Pulliam, Sarah E; Veronese, Paola; Shantharaj, Deepak; Nazir, Azka; Jacobs, Nekaiya S; Stratmann, Johannes W

    2011-02-01

    The COP9 signalosome (CSN) is a multi-protein complex that regulates the activities of cullin-RING E3 ubiquitin ligases (CRLs). CRLs ubiquitinate proteins in order to target them for proteasomal degradation. The CSN is required for proper plant development. Here we show that the CSN also has a profound effect on plant defense responses. Silencing of genes for CSN subunits in tomato plants resulted in a mild morphological phenotype and reduced expression of wound-responsive genes in response to mechanical wounding, attack by Manduca sexta larvae, and Prosystemin over-expression. In contrast, expression of pathogenesis-related genes was increased in a stimulus-independent manner in these plants. The reduced wound response in CSN-silenced plants corresponded with reduced synthesis of jasmonic acid (JA), but levels of salicylic acid (SA) were unaltered. As a consequence, these plants exhibited reduced resistance against herbivorous M. sexta larvae and the necrotrophic fungal pathogen Botrytis cinerea. In contrast, susceptibility to tobacco mosaic virus (TMV) was not altered in CSN-silenced plants. These data demonstrate that the CSN orchestrates not only plant development but also JA-dependent plant defense responses. © 2011 The Authors. The Plant Journal © 2011 Blackwell Publishing Ltd.

  4. Initiation of farm safety programs in the Arkansas Delta: a case study of participatory methods.

    PubMed

    Richter, Jan S; Hall, Becky G; Deere, G David

    2007-01-01

    Outreach to high-risk communities is one of the goals of Area Health Education Centers. One such population is the farm community, which is known to suffer high rates of traumatic events. To describe a participatory methods initiative by the Arkansas Delta Area Health Education Center and other agencies to address farm-related health hazards in a 7-county region. Regional injury and fatality data were gathered from sources including Arkansas Farm Bureau Federation insurance claims, the Arkansas Statistical Service Phone Survey, the National Agricultural Statistics Service, and the Cooperative Extension Service Division of Agriculture at the University of Arkansas. Focus groups were held to assess farmer perceptions and recommendations. Accidents involving tractors accounted for 42% of deaths, and accidents with crop-spraying aircraft accounted for 36%. Focus group participants agreed that planting and harvesting seasons were particularly dangerous. Recommendations included educating motorists to be more cautious on agricultural area roads, using local farmers to provide farm safety training, and making safety equipment more available.

  5. California Solar Farms

    NASA Image and Video Library

    2015-04-02

    On February 15, 2015 the Desert Sunlight solar project in California’s Mojave Desert became operational. This image from NASA Terra spacecraft shows the 550-megawatt plant generates enough electricity to power 160,000 average homes. Covering an area of 16 km2, the 8.8 million cadmium telluride photovoltaic modules take advantage of the more than 300 days of sunshine. Desert Sunlight joins the similar-sized Topaz Solar Farm in San Luis Obispo County, CA, that became operational in June, 2014. The Desert Sunlight image (left) was acquired March 12, 2015 and is located at 33.8 degrees north, 115.4 degrees west; the Topaz image (right) was acquired September 11, 2014 and is located at 35.4 degrees north, 120.1 degrees west. Each image covers an area of 10.5 x 12 km. http://photojournal.jpl.nasa.gov/catalog/PIA19268

  6. 29 CFR 500.41 - Farm labor contractor is responsible for actions of his farm labor contractor employee.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ..., prior to such employee's engagement in any activity enumerated in section 3(6) of the Act. A farm labor... farm labor contractor employee. 500.41 Section 500.41 Labor Regulations Relating to Labor (Continued... PROTECTION Registration of Farm Labor Contractors and Employees of Farm Labor Contractors Engaged in Farm...

  7. 29 CFR 500.41 - Farm labor contractor is responsible for actions of his farm labor contractor employee.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ..., prior to such employee's engagement in any activity enumerated in section 3(6) of the Act. A farm labor... farm labor contractor employee. 500.41 Section 500.41 Labor Regulations Relating to Labor (Continued... PROTECTION Registration of Farm Labor Contractors and Employees of Farm Labor Contractors Engaged in Farm...

  8. Hazards of Farming

    PubMed Central

    Guilfoyle, John

    1992-01-01

    Farming is the most dangerous occupation in the industrialized world. Children, in particular, are at high risk for injury and disability. There is ample scope to improve this situation. Parents are the most important group to be educated. Emergency response services in rural areas are sometimes unable to provide optimum care for victims. Better surveillance methods need to be in place, both to gather information and to evaluate strategies aimed at prevention. Farm safety needs to be higher on the agenda for farmers, farm organizations, government, and health care professionals. PMID:21221275

  9. A Stated Preference Investigation into the Chinese Demand for Farmed vs. Wild Bear Bile

    PubMed Central

    Dutton, Adam J.; Hepburn, Cameron; Macdonald, David W.

    2011-01-01

    Farming of animals and plants has recently been considered not merely as a more efficient and plentiful supply of their products but also as a means of protecting wild populations from that trade. Amongst these nascent farming products might be listed bear bile. Bear bile has been exploited by traditional Chinese medicinalists for millennia. Since the 1980s consumers have had the options of: illegal wild gall bladders, bile extracted from caged live bears or the acid synthesised chemically. Despite these alternatives bears continue to be harvested from the wild. In this paper we use stated preference techniques using a random sample of the Chinese population to estimate demand functions for wild bear bile with and without competition from farmed bear bile. We find a willingness to pay considerably more for wild bear bile than farmed. Wild bear bile has low own price elasticity and cross price elasticity with farmed bear bile. The ability of farmed bear bile to reduce demand for wild bear bile is at best limited and, at prevailing prices, may be close to zero or have the opposite effect. The demand functions estimated suggest that the own price elasticity of wild bear bile is lower when competing with farmed bear bile than when it is the only option available. This means that the incumbent product may actually sell more items at a higher price when competing than when alone in the market. This finding may be of broader interest to behavioural economists as we argue that one explanation may be that as product choice increases price has less impact on decision making. For the wildlife farming debate this indicates that at some prices the introduction of farmed competition might increase the demand for the wild product. PMID:21799733

  10. A stated preference investigation into the Chinese demand for farmed vs. wild bear bile.

    PubMed

    Dutton, Adam J; Hepburn, Cameron; Macdonald, David W

    2011-01-01

    Farming of animals and plants has recently been considered not merely as a more efficient and plentiful supply of their products but also as a means of protecting wild populations from that trade. Amongst these nascent farming products might be listed bear bile. Bear bile has been exploited by traditional Chinese medicinalists for millennia. Since the 1980s consumers have had the options of: illegal wild gall bladders, bile extracted from caged live bears or the acid synthesised chemically. Despite these alternatives bears continue to be harvested from the wild. In this paper we use stated preference techniques using a random sample of the Chinese population to estimate demand functions for wild bear bile with and without competition from farmed bear bile. We find a willingness to pay considerably more for wild bear bile than farmed. Wild bear bile has low own price elasticity and cross price elasticity with farmed bear bile. The ability of farmed bear bile to reduce demand for wild bear bile is at best limited and, at prevailing prices, may be close to zero or have the opposite effect. The demand functions estimated suggest that the own price elasticity of wild bear bile is lower when competing with farmed bear bile than when it is the only option available. This means that the incumbent product may actually sell more items at a higher price when competing than when alone in the market. This finding may be of broader interest to behavioural economists as we argue that one explanation may be that as product choice increases price has less impact on decision making. For the wildlife farming debate this indicates that at some prices the introduction of farmed competition might increase the demand for the wild product.

  11. Green Care: A Review of the Benefits and Potential of Animal-Assisted Care Farming Globally and in Rural America.

    PubMed

    Artz, Brianna; Bitler Davis, Doris

    2017-04-13

    The term Green Care includes therapeutic, social or educational interventions involving farming; farm animals; gardening or general contact with nature. Although Green Care can occur in any setting in which there is interaction with plants or animals, this review focuses on therapeutic practices occurring on farms. The efficacy of care farming is discussed and the broad utilization of care farming and farm care communities in Europe is reviewed. Though evidence from care farms in the United States is included in this review, the empirical evidence which could determine its efficacy is lacking. For example, the empirical evidence supporting or refuting the efficacy of therapeutic horseback riding in adults is minimal, while there is little non-equine care farming literature with children. The health care systems in Europe are also much different than those in the United States. In order for insurance companies to cover Green Care techniques in the United States, extensive research is necessary. This paper proposes community-based ways that Green Care methods can be utilized without insurance in the United States. Though Green Care can certainly be provided in urban areas, this paper focuses on ways rural areas can utilize existing farms to benefit the mental and physical health of their communities.

  12. Analysis of Farm Records. Teacher Edition. Farm Business Management Series.

    ERIC Educational Resources Information Center

    Oklahoma State Dept. of Vocational and Technical Education, Stillwater. Curriculum and Instructional Materials Center.

    This curriculum guide for the Oklahoma Farm Business Management Program contains three instructional units that teach students how to interpret farm records to get optimum use of facilities and maximize profits. Each unit of instruction includes some or all of these components: performance objectives, suggested activities for the instructor,…

  13. Whole Farm Nutrient Balance Calculator for New York Dairy Farms

    ERIC Educational Resources Information Center

    Soberon, Melanie A.; Ketterings, Quirine M.; Rasmussen, Caroline N.; Czymmek, Karl J.

    2013-01-01

    Nutrient loss and accumulation as well as associated environmental degradation have been a concern for animal agriculture for many decades. Federal and New York (NY) regulations apply to Concentrated Animal Feeding Operations and a comprehensive nutrient management plan (CNMP) is required for regulated farms. The whole farm nutrient mass balance…

  14. Modulation of ethylene- and heat-controlled hyponastic leaf movement in Arabidopsis thaliana by the plant defence hormones jasmonate and salicylate.

    PubMed

    van Zanten, Martijn; Ritsema, Tita; Polko, Joanna K; Leon-Reyes, Antonio; Voesenek, Laurentius A C J; Millenaar, Frank F; Pieterse, Corné M J; Peeters, Anton J M

    2012-04-01

    Upward leaf movement (hyponastic growth) is adopted by several plant species including Arabidopsis thaliana, as a mechanism to escape adverse growth conditions. Among the signals that trigger hyponastic growth are, the gaseous hormone ethylene, low light intensities, and supra-optimal temperatures (heat). Recent studies indicated that the defence-related phytohormones jasmonic acid (JA) and salicylic acid (SA) synthesized by the plant upon biotic infestation repress low light-induced hyponastic growth. The hyponastic growth response induced by high temperature (heat) treatment and upon application of the gaseous hormone ethylene is highly similar to the response induced by low light. To test if these environmental signals induce hyponastic growth via parallel pathways or converge downstream, we studied here the roles of Methyl-JA (MeJA) and SA on ethylene- and heat-induced hyponastic growth. For this, we used a time-lapse camera setup. Our study includes pharmacological application of MeJA and SA and biological infestation using the JA-inducing caterpillar Pieris rapae as well as mutants lacking JA or SA signalling components. The data demonstrate that MeJA is a positive, and SA, a negative regulator of ethylene-induced hyponastic growth and that both hormones repress the response to heat. Taking previous studies into account, we conclude that SA is the first among many tested components which is repressing hyponastic growth under all tested inductive environmental stimuli. However, since MeJA is a positive regulator of ethylene-induced hyponastic growth and is inhibiting low light- and heat-induced leaf movement, we conclude that defence hormones control hyponastic growth by affecting stimulus-specific signalling pathways.

  15. Farm Building Contractors and Manufacturers: Their Role in Midwest Farm Construction

    Treesearch

    Lyle Solverson; David C. Baumgartner

    1974-01-01

    Describes and analyzes the activities of farm building contractors and manufacturers in Illinois, Indiana, Iowa, and Missouri. Contractors and manufacturers are setting the trend in the farm building market in terms of building type and size and materials used.

  16. Elevated O3 increases volatile organic compounds via jasmonic acid pathway that promote the preference of parasitoid Encarsia formosa for tomato plants.

    PubMed

    Cui, Hongying; Wei, Jianing; Su, Jianwei; Li, Chuanyou; Ge, Feng

    2016-12-01

    The elevated atmospheric O 3 level may change the interactions of plants and insects, which potentially affects direct and indirect plant defences. However, the underlying mechanism of the impact of elevated O 3 on indirect plant defence, namely the efficacy of natural enemies, is unclear. Here we tested a hypothesis that linked the effects of elevated O 3 and whitefly herbivory on tomato volatile releases mediated by the jasmonic acid (JA) pathway with the preferences of parasitoid Encarsia formosa for two different tomato genotypes (wild-type (Wt) and JA-deficient genotype (spr2)). The O 3 and whitefly herbivory significantly increased the production of volatile organic compounds (VOCs), including monoterpenes and green leaf volatiles (GLVs). The Wt plants released higher volatile levels, particularly monoterpenes, than did the spr2 plants. In Y-tube tests, limonene and Z-3-hexanol played key roles in the attraction of E. formosa. Moreover, regardless of plant genotype, the two plant genotypes were preferred by adult E. formosa under the O 3 and O 3 + herbivory treatments. Our results suggest that under elevated O 3 , the activation of the JA pathway significantly up-regulates the emission rates of volatiles, through which the efficacy of natural enemy might be promoted. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

  17. Farm Women, Farming Systems, and Agricultural Structure: Suggestions for Scholarship.

    ERIC Educational Resources Information Center

    Flora, Cornelia Butler

    1981-01-01

    Suggests research agenda to analyze the class struggle occurring with farm women. Views the household as the unit of analysis, both internally from a farming-systems perspective and externally as responding to shifts in policy and technology. Available from: Rural Sociological Society, 325 Morgan Hall, University of Tennessee, Knoxville, TN 37916.…

  18. Finding Farms: Comparing Indicators of Farming Dependence and Agricultural Importance in the United States

    ERIC Educational Resources Information Center

    Jackson-Smith, Douglas B.; Jensen, Eric

    2009-01-01

    Many scholars have commented on the changing significance of farming for understanding the dynamics of social and economic change in contemporary rural America. Quantitative analyses of relationships between farming, local socioeconomic conditions, demographic trends, and policy have often relied on an indicator of "farm-dependent" (FD) counties…

  19. 29 CFR 780.207 - Operations with respect to wild plants.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 29 Labor 3 2014-07-01 2014-07-01 false Operations with respect to wild plants. 780.207 Section 780... respect to wild plants. Nurseries frequently obtain plants growing wild in the woods or fields which are... these activities off the farm would not be engaged in agriculture. The transplanting of such wild plants...

  20. 29 CFR 780.207 - Operations with respect to wild plants.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 29 Labor 3 2011-07-01 2011-07-01 false Operations with respect to wild plants. 780.207 Section 780... respect to wild plants. Nurseries frequently obtain plants growing wild in the woods or fields which are... these activities off the farm would not be engaged in agriculture. The transplanting of such wild plants...

  1. 29 CFR 780.207 - Operations with respect to wild plants.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 29 Labor 3 2012-07-01 2012-07-01 false Operations with respect to wild plants. 780.207 Section 780... respect to wild plants. Nurseries frequently obtain plants growing wild in the woods or fields which are... these activities off the farm would not be engaged in agriculture. The transplanting of such wild plants...

  2. 29 CFR 780.207 - Operations with respect to wild plants.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 29 Labor 3 2013-07-01 2013-07-01 false Operations with respect to wild plants. 780.207 Section 780... respect to wild plants. Nurseries frequently obtain plants growing wild in the woods or fields which are... these activities off the farm would not be engaged in agriculture. The transplanting of such wild plants...

  3. [Characteristics of paddy field nitrogen leakage and runoff in rice-duck farming system].

    PubMed

    Yu, Xiang; Wang, Qiang-sheng; Wang, Shao-hua; Liu, Zheng-hui; Wang, Xia-wen; Ding, Yan-feng

    2009-01-01

    A field experiment was conducted to study the characteristics of paddy field nitrogen (N) leakage and runoff under rice-duck farming (MRD), conventional farming (MR), and conventional farming with flooding (CK). Comparing with that under MR, the paddy field under MRD had a notable decrease of N (especially NO3- -N) concentration in its leaked liquid; but this concentration was tended to be increased, compared with that under CK. After 7-9 days of fertilization, the NH4+ -N and NO3- -N concentrations in paddy field surface water were higher under MRD than under MR. However, owing to the no draining and the higher band, the paddy field under MRD had a notable reduction of drainage, resulting in a marked decrease of N runoff than that under MR. Comparing with MR, the paddy field under MRD had an addition of nitrogen supply from duck dung, a reduction of N leakage and runoff, a lesser application of chemical nitrogen fertilizer, and more nitrogen uptake by rice plant. Both the reduction of N input and that of N output in rice-duck farming system were nearly equal in quantity.

  4. A simple rule based model for scheduling farm management operations in SWAT

    NASA Astrophysics Data System (ADS)

    Schürz, Christoph; Mehdi, Bano; Schulz, Karsten

    2016-04-01

    For many interdisciplinary questions at the watershed scale, the Soil and Water Assessment Tool (SWAT; Arnold et al., 1998) has become an accepted and widely used tool. Despite its flexibility, the model is highly demanding when it comes to input data. At SWAT's core the water balance and the modeled nutrient cycles are plant growth driven (implemented with the EPIC crop growth model). Therefore, land use and crop data with high spatial and thematic resolution, as well as detailed information on cultivation and farm management practices are required. For many applications of the model however, these data are unavailable. In order to meet these requirements, SWAT offers the option to trigger scheduled farm management operations by applying the Potential Heat Unit (PHU) concept. The PHU concept solely takes into account the accumulation of daily mean temperature for management scheduling. Hence, it contradicts several farming strategies that take place in reality; such as: i) Planting and harvesting dates are set much too early or too late, as the PHU concept is strongly sensitivity to inter-annual temperature fluctuations; ii) The timing of fertilizer application, in SWAT this often occurs simultaneously on the same date in in each field; iii) and can also coincide with precipitation events. Particularly, the latter two can lead to strong peaks in modeled nutrient loads. To cope with these shortcomings we propose a simple rule based model (RBM) to schedule management operations according to realistic farmer management practices in SWAT. The RBM involves simple strategies requiring only data that are input into the SWAT model initially, such as temperature and precipitation data. The user provides boundaries of time periods for operation schedules to take place for all crops in the model. These data are readily available from the literature or from crop variety trials. The RBM applies the dates by complying with the following rules: i) Operations scheduled in the

  5. Arabidopsis histone methyltransferase SET DOMAIN GROUP8 mediates induction of the jasmonate/ethylene pathway genes in plant defense response to necrotrophic fungi.

    PubMed

    Berr, Alexandre; McCallum, Emily J; Alioua, Abdelmalek; Heintz, Dimitri; Heitz, Thierry; Shen, Wen-Hui

    2010-11-01

    As sessile organisms, plants have to endure a wide variety of biotic and abiotic stresses, and accordingly they have evolved intricate and rapidly inducible defense strategies associated with the activation of a battery of genes. Among other mechanisms, changes in chromatin structure are thought to provide a flexible, global, and stable means for the regulation of gene transcription. In support of this idea, we demonstrate here that the Arabidopsis (Arabidopsis thaliana) histone methyltransferase SET DOMAIN GROUP8 (SDG8) plays a crucial role in plant defense against fungal pathogens by regulating a subset of genes within the jasmonic acid (JA) and/or ethylene signaling pathway. We show that the loss-of-function mutant sdg8-1 displays reduced resistance to the necrotrophic fungal pathogens Alternaria brassicicola and Botrytis cinerea. While levels of JA, a primary phytohormone involved in plant defense, and camalexin, a major phytoalexin against fungal pathogens, remain unchanged or even above normal in sdg8-1, induction of several defense genes within the JA/ethylene signaling pathway is severely compromised in response to fungal infection or JA treatment in mutant plants. Both downstream genes and, remarkably, also upstream mitogen-activated protein kinase kinase genes MKK3 and MKK5 are misregulated in sdg8-1. Accordingly, chromatin immunoprecipitation analysis shows that sdg8-1 impairs dynamic changes of histone H3 lysine 36 methylation at defense marker genes as well as at MKK3 and MKK5, which normally occurs upon infection with fungal pathogens or methyl JA treatment in wild-type plants. Our data indicate that SDG8-mediated histone H3 lysine 36 methylation may serve as a memory of permissive transcription for a subset of defense genes, allowing rapid establishment of transcriptional induction.

  6. Economic Indicators of the Farm Sector. Farm Sector Review, 1985.

    ERIC Educational Resources Information Center

    Economic Research Service (USDA), Washington, DC.

    Farm production rose 6 percent in 1985 due to record high yields in corn, soybeans, cotton, and several other crops. While United States consumption increased slightly, exports of farm products fell 23 percent in value and 19 percent in volume. Net cash income increased 12 percent due to increased output, lower cash expenses, and unusually high…

  7. Ships as future floating farm systems?

    PubMed

    Moustafa, Khaled

    2018-04-03

    Environmental and agriculture challenges such as severe drought, desertification, sprawling cities and shrinking arable lands in large regions in the world compel us to think about alternative and sustainable farming systems. Ongoing projects to build floating cities in the sea suggest that building specific ships for farming purposes (as farming ships or farming boats) would also be attainable to introduce new farming surfaces and boost food production worldwide to cope with food insecurity issues.

  8. Monitoring and remediation of on-farm and off-farm ground current measured as step potential on a Wisconsin dairy farm: A case study.

    PubMed

    Stetzer, Dave; Leavitt, Adam M; Goeke, Charles L; Havas, Magda

    2016-01-01

    Ground current commonly referred to as "stray voltage" has been an issue on dairy farms since electricity was first brought to rural America. Equipment that generates high-frequency voltage transients on electrical wires combined with a multigrounded (electrical distribution) system and inadequate neutral returns all contribute to ground current. Despite decades of problems, we are no closer to resolving this issue, in part, due to three misconceptions that are addressed in this study. Misconception 1. The current standard of 1 V at cow contact is adequate to protect dairy cows; Misconception 2. Frequencies higher than 60 Hz do not need to be considered; and Misconception 3. All sources of ground current originate on the farm that has a ground current problem. This case study of a Wisconsin dairy farm documents, 1. how to establish permanent monitoring of ground current (step potential) on a dairy farm; 2. how to determine and remediate both on-farm and off-farm sources contributing to step potential; 3. which step-potential metrics relate to cow comfort and milk production; and 4. how these metrics relate to established standards. On-farm sources include lighting, variable speed frequency drives on motors, radio frequency identification system and off-farm sources are due to a poor primary neutral return on the utility side of the distribution system. A step-potential threshold of 1 V root mean square (RMS) at 60 Hz is inadequate to protect dairy cows as decreases of a few mV peak-peak at higher frequencies increases milk production, reduces milking time and improves cow comfort.

  9. Control voltage and power fluctuations when connecting wind farms

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Berinde, Ioan, E-mail: ioan-berinde@yahoo.com; Bălan, Horia, E-mail: hbalan@mail.utcluj.ro; Oros, Teodora Susana, E-mail: teodoraoros-87@yahoo.com

    2015-12-23

    Voltage, frequency, active power and reactive power are very important parameters in terms of power quality. These parameters are followed when connecting any power plant, the more the connection of wind farms. Connecting wind farms to the electricity system must not cause interference outside the limits set by regulations. Modern solutions for fast and automatic voltage control and power fluctuations using electronic control systems of reactive power flows. FACTS (Flexible Alternating Current Transmision System) systems, established on the basis of power electronic circuits ensure control of electrical status quantities to achieve the necessary transfer of power to the power grid.more » FACTS devices can quickly control parameters and sizes of state power lines, such as impedance line voltages and phase angles of the voltages of the two ends of the line. Their use can lead to improvement in power system operation by increasing the transmission capacity of power lines, power flow control lines, improved static and transient stability reserve.« less

  10. Control voltage and power fluctuations when connecting wind farms

    NASA Astrophysics Data System (ADS)

    Berinde, Ioan; Bǎlan, Horia; Oros Pop, Teodora Susana

    2015-12-01

    Voltage, frequency, active power and reactive power are very important parameters in terms of power quality. These parameters are followed when connecting any power plant, the more the connection of wind farms. Connecting wind farms to the electricity system must not cause interference outside the limits set by regulations. Modern solutions for fast and automatic voltage control and power fluctuations using electronic control systems of reactive power flows. FACTS (Flexible Alternating Current Transmision System) systems, established on the basis of power electronic circuits ensure control of electrical status quantities to achieve the necessary transfer of power to the power grid. FACTS devices can quickly control parameters and sizes of state power lines, such as impedance line voltages and phase angles of the voltages of the two ends of the line. Their use can lead to improvement in power system operation by increasing the transmission capacity of power lines, power flow control lines, improved static and transient stability reserve.

  11. [Pyrrolizidine alkaloids and seneciosis in farm animals. Part 1: occurrence, chemistry and toxicology].

    PubMed

    Petzinger, E

    2011-01-01

    Pyrrolizidine alkaloids belong to a class of phytotoxins which are present in more than 6000 plant species. The disease seneciosis in farm animals represents the severe poisoning by pyrrolizidine alkaloids from plants of the genus Senecio. This form of poisoning has been known since the end of the 19th century in Germany, the USA, Canada and New Zealand, and is mainly caused by Senecio jacobaea and related Senecio spp. in farm animals, including poultry. Animal poisoning by pyrrolizidine alkaloids is of worldwide importance. In Germany poisoning of horses and cattle by Senecio jacobaea, which was earlier named Schweinsberg disease, is of renewed relevance for veterinary medicine. The disease occurs almost entirely as a consequence of chronic poisoning and in general ends fatally. The ultimate cause is the formation of toxic metabolites of pyrrolizidine alkaloids in the liver, and their covalent binding to nucleic acids and proteins leading to liver cirrhosis. Because many pyrrolizidine alkaloids possess mutagenic, and a few also carcinogenic properties, European and international authorities are concerned about possible residue levels in food of animal origin. The review addresses in its first part several aspects, being the occurrence, the chemistry, and the toxicology of pyrrolizidine alkaloids as well as animal intoxications by poisonous plants. In the second part (46) clinical characteristics of animal seneciosis, the therapeutic interventions, the significant species differences and a critical assessment of so-called nontoxic amounts of Senecio plants in animal fodder with reference to cumulative lethal toxin doses are presented.

  12. 7 CFR 718.201 - Farm constitution.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 7 2010-01-01 2010-01-01 false Farm constitution. 718.201 Section 718.201 Agriculture... Reconstitution of Farms, Allotments, Quotas, and Bases § 718.201 Farm constitution. (a) In order to implement... this section. The constitution and identification of land as a farm for the first time and the...

  13. 7 CFR 718.201 - Farm constitution.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 7 2012-01-01 2012-01-01 false Farm constitution. 718.201 Section 718.201 Agriculture... Reconstitution of Farms, Allotments, Quotas, and Bases § 718.201 Farm constitution. (a) In order to implement... this section. The constitution and identification of land as a farm for the first time and the...

  14. 7 CFR 718.201 - Farm constitution.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 7 2013-01-01 2013-01-01 false Farm constitution. 718.201 Section 718.201 Agriculture... Reconstitution of Farms, Allotments, Quotas, and Bases § 718.201 Farm constitution. (a) In order to implement... this section. The constitution and identification of land as a farm for the first time and the...

  15. 7 CFR 718.201 - Farm constitution.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 7 2011-01-01 2011-01-01 false Farm constitution. 718.201 Section 718.201 Agriculture... Reconstitution of Farms, Allotments, Quotas, and Bases § 718.201 Farm constitution. (a) In order to implement... this section. The constitution and identification of land as a farm for the first time and the...

  16. 7 CFR 718.201 - Farm constitution.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 7 2014-01-01 2014-01-01 false Farm constitution. 718.201 Section 718.201 Agriculture... Reconstitution of Farms, Allotments, Quotas, and Bases § 718.201 Farm constitution. (a) In order to implement... this section. The constitution and identification of land as a farm for the first time and the...

  17. Constitutive Expression of Mammalian Nitric Oxide Synthase in Tobacco Plants Triggers Disease Resistance to Pathogens

    PubMed Central

    Chun, Hyun Jin; Park, Hyeong Cheol; Koo, Sung Cheol; Lee, Ju Huck; Park, Chan Young; Choi, Man Soo; Kang, Chang Ho; Baek, Dongwon; Cheong, Yong Hwa; Yun, Dae-Jin; Chung, Woo Sik; Cho, Moo Je; Kim, Min Chul

    2012-01-01

    Nitric oxide (NO) is known for its role in the activation of plant defense responses. To examine the involvement and mode of action of NO in plant defense responses, we introduced calmodulin-dependent mammalian neuronal nitric oxide synthase (nNOS), which controls the CaMV35S promoter, into wild-type and NahG tobacco plants. Constitutive expression of nNOS led to NO production and triggered spontaneous induction of leaf lesions. Transgenic plants accumulated high amounts of H2O2, with catalase activity lower than that in the wild type. nNOS transgenic plants contained high levels of salicylic acid (SA), and they induced an array of SA-, jasmonic acid (JA)-, and/or ethylene (ET)-related genes. Consequently, NahG co-expression blocked the induction of systemic acquired resistance (SAR)-associated genes in transgenic plants, implying SA is involved in NO-mediated induction of SAR genes. The transgenic plants exhibited enhanced resistance to a spectrum of pathogens, including bacteria, fungi, and viruses. Our results suggest a highly ranked regulatory role for NO in SA-, JA-, and/or ET-dependent pathways that lead to disease resistance. PMID:23124383

  18. Farm Education at Stony Kill.

    ERIC Educational Resources Information Center

    Parisio, Richard

    1986-01-01

    Describes typical winter farm lessons for students visiting Stony Kill Farm Environmental Education Center located 70 miles north of New York City: butter and corncake making, soil erosion experiments, dissecting and growing seeds. Emphasizes major theme of conservation of farmland from destructive farming practices and careless development. (NEC)

  19. An Integrated Method to Analyze Farm Vulnerability to Climatic and Economic Variability According to Farm Configurations and Farmers' Adaptations.

    PubMed

    Martin, Guillaume; Magne, Marie-Angélina; Cristobal, Magali San

    2017-01-01

    The need to adapt to decrease farm vulnerability to adverse contextual events has been extensively discussed on a theoretical basis. We developed an integrated and operational method to assess farm vulnerability to multiple and interacting contextual changes and explain how this vulnerability can best be reduced according to farm configurations and farmers' technical adaptations over time. Our method considers farm vulnerability as a function of the raw measurements of vulnerability variables (e.g., economic efficiency of production), the slope of the linear regression of these measurements over time, and the residuals of this linear regression. The last two are extracted from linear mixed models considering a random regression coefficient (an intercept common to all farms), a global trend (a slope common to all farms), a random deviation from the general mean for each farm, and a random deviation from the general trend for each farm. Among all possible combinations, the lowest farm vulnerability is obtained through a combination of high values of measurements, a stable or increasing trend and low variability for all vulnerability variables considered. Our method enables relating the measurements, trends and residuals of vulnerability variables to explanatory variables that illustrate farm exposure to climatic and economic variability, initial farm configurations and farmers' technical adaptations over time. We applied our method to 19 cattle (beef, dairy, and mixed) farms over the period 2008-2013. Selected vulnerability variables, i.e., farm productivity and economic efficiency, varied greatly among cattle farms and across years, with means ranging from 43.0 to 270.0 kg protein/ha and 29.4-66.0% efficiency, respectively. No farm had a high level, stable or increasing trend and low residuals for both farm productivity and economic efficiency of production. Thus, the least vulnerable farms represented a compromise among measurement value, trend, and variability of

  20. An Integrated Method to Analyze Farm Vulnerability to Climatic and Economic Variability According to Farm Configurations and Farmers’ Adaptations

    PubMed Central

    Martin, Guillaume; Magne, Marie-Angélina; Cristobal, Magali San

    2017-01-01

    The need to adapt to decrease farm vulnerability to adverse contextual events has been extensively discussed on a theoretical basis. We developed an integrated and operational method to assess farm vulnerability to multiple and interacting contextual changes and explain how this vulnerability can best be reduced according to farm configurations and farmers’ technical adaptations over time. Our method considers farm vulnerability as a function of the raw measurements of vulnerability variables (e.g., economic efficiency of production), the slope of the linear regression of these measurements over time, and the residuals of this linear regression. The last two are extracted from linear mixed models considering a random regression coefficient (an intercept common to all farms), a global trend (a slope common to all farms), a random deviation from the general mean for each farm, and a random deviation from the general trend for each farm. Among all possible combinations, the lowest farm vulnerability is obtained through a combination of high values of measurements, a stable or increasing trend and low variability for all vulnerability variables considered. Our method enables relating the measurements, trends and residuals of vulnerability variables to explanatory variables that illustrate farm exposure to climatic and economic variability, initial farm configurations and farmers’ technical adaptations over time. We applied our method to 19 cattle (beef, dairy, and mixed) farms over the period 2008–2013. Selected vulnerability variables, i.e., farm productivity and economic efficiency, varied greatly among cattle farms and across years, with means ranging from 43.0 to 270.0 kg protein/ha and 29.4–66.0% efficiency, respectively. No farm had a high level, stable or increasing trend and low residuals for both farm productivity and economic efficiency of production. Thus, the least vulnerable farms represented a compromise among measurement value, trend, and

  1. 26 CFR 20.2032A-3 - Material participation requirements for valuation of certain farm and closely-held business real...

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... successive interests in specially valued property (e.g. life estate and remainder interests) from the...-year old widow of farmer C. She lives on a farm for which special valuation has been elected and has... what fields to plant and when to plant and harvest crops are left to E, but D does occasionally make...

  2. Farming-Dependent Counties and the Financial Well-Being of Farm Operator Households. Agriculture Information Bulletin Number 544.

    ERIC Educational Resources Information Center

    Ahearn, Mary; And Others

    The financial well-being of farmers varies with the local economy's dependence on farming. Farming-dependent counties (where farming contributes at least 20 percent of the county's total earnings in 1980-84), offer limited nonfarm employment opportunities. These operators earn high farm incomes but face large debts. Declining land values have…

  3. Content Priorities for Farm Mechanics

    ERIC Educational Resources Information Center

    Knotts, C. Don; Webb, Earl S.

    1974-01-01

    Fifty successful young Texas farmers evaluated agricultural mechanics skills (in the broad areas of farm power and machinery, farm shop, farm electricity, buildings and conveniences, and soil and water management) in terms of their importance. Teachers can use the findings to plan course content relevant to their students' needs. (AJ)

  4. Food metabolomics: from farm to human.

    PubMed

    Kim, Sooah; Kim, Jungyeon; Yun, Eun Ju; Kim, Kyoung Heon

    2016-02-01

    Metabolomics, one of the latest components in the suite of systems biology, has been used to understand the metabolism and physiology of living systems, including microorganisms, plants, animals and humans. Food metabolomics can be defined as the application of metabolomics in food systems, including food resources, food processing and diet for humans. The study of food metabolomics has increased gradually in the recent years, because food systems are directly related to nutrition and human health. This review describes the recent trends and applications of metabolomics to food systems, from farm to human, including food resource production, industrial food processing and food intake by humans. Copyright © 2015 Elsevier Ltd. All rights reserved.

  5. Dairying. People on the Farm. [Revised].

    ERIC Educational Resources Information Center

    Department of Agriculture, Washington, DC. Office of Governmental and Public Affairs.

    This booklet, one in a series about life on modern farms, describes the daily lives of two dairy farm families, the Schwartzbecks and the Bealls of Maryland. Beginning with early morning milking, the booklet traces the farm families through their daily work and community activities, explaining how a modern dairy farm is run. Although this booklet…

  6. Black Families. People on the Farm.

    ERIC Educational Resources Information Center

    Department of Agriculture, Washington, DC. Office of Governmental and Public Affairs.

    This booklet, one in a series about life on modern farms, describes the daily life of two black farm families, the Minisees of Michigan and the Sims brothers of Arkansas. Beginning with early morning chores, the booklet describes some of the chores they do in running their diversified farms and how they got started in farming. Although the booklet…

  7. INSTRUCTION IN FARM MECHANICS, SUGGESTIONS FOR DEVELOPING TRAINING PROGRAMS IN FARM MECHANICS IN VOCATIONAL AGRICULTURE.

    ERIC Educational Resources Information Center

    HOLLENBERG, A.H.; JOHNSON, E.J.

    THE PURPOSE OF THE PROGRAM GUIDE IS TO ASSIST TEACHERS IN TRAINING YOUNG FARMERS AND FARM WORKERS IN THE SELECTION, OPERATION, UTILIZATION, AND MAINTENANCE OF FARM TOOLS, MACHINERY, AND MECHANICAL EQUIPMENT. DESIGNED BY NATIONAL AGRICULTURAL EDUCATION SPECIALISTS, THE DOCUMENT INCLUDES CHAPTERS ON THE CHANGING FARM, SETTING UP PROGRAMS, FARM…

  8. Seventeen-Year Growth of Cherrybark Oak and Loblolly Pine on a Previously Farmed Bottomland Site

    Treesearch

    Warren D. Devine; John C. Rennie; Allan E. Houston; Donald D. Tyler; Vernon H. Reich

    2002-01-01

    This study documents the effects of cultural treatments on 17-year growth of cherrybark oak (Quercus pagoda Raf.) and lobiolly pine (Pinus taeda L.) planted on a previously farmed bottomland site in southwestern Tennessee. Yellow-poplar (Liriodendron tulipifera L.) was part of the original study, but was...

  9. Field application of farm-food safety risk assessment (FRAMp) tool for small and medium fresh produce farms.

    PubMed

    Soon, J M; Davies, W P; Chadd, S A; Baines, R N

    2013-02-15

    The objective of this study was to develop a farm food safety-risk assessment tool (FRAMp) which serves as a self-assessment and educational tool for fresh produce farms. FRAMp was developed in Microsoft® Excel spreadsheet software using standard mathematical and logical functions and utilised a qualitative risk assessment approach for farmers to evaluate their food safety practices. The FRAMp tool has since been tested on 12 fresh produce farms throughout UK. All the farms determined that FRAMp was interesting but 17% found it too long while 25% of the farms felt the tool was too complicated. The instructions on FRAMp usage were revised and farmers were given the options to skip and select specific steps in the farm risk assessment. The end users (farmers/farm managers) determined that developing their own action plans and using it as proof of assessment for future third-party audits were most useful to them. FRAMp tool can be described as an illustrative risk ranking tool to facilitate farms to identify potential risk factors during their crop production. Copyright © 2012 Elsevier Ltd. All rights reserved.

  10. Livestock farming and atmospheric emissions.

    PubMed

    Zicari, Giuseppe; Soardo, Vincenzo; Rivetti, Daniela; Cerrato, Elena; Russo, Domenico

    2013-01-01

    Livestock farming produces atmospheric emissions that may pose a risk to workers and a disturbance to the population. Emissions into the atmosphere produced by livestock farming consist of gases such as ammonia, dust, compounds such as aliphatic hydrocarbons and bio-aerosols formed by microorganisms. Some gases, such as ammonia and hydrogen sulphide, have foul odours and are thus potentially annoying to the population. Gaseous or volatile molecules produced by livestock installations and related activities may have several adverse effects on health and environment. The most significant exposure certainly relates to workers in the confined spaces of farms, rather than to residents in the surrounding areas. In this article we examine potential hazards to farm workers and to the population living in the vicinity of livestock farms, arising from emissions into the atmosphere.

  11. Organic farming enhances soil microbial abundance and activity—A meta-analysis and meta-regression

    PubMed Central

    Symnaczik, Sarah; Mäder, Paul; De Deyn, Gerlinde; Gattinger, Andreas

    2017-01-01

    between organic and conventional farming systems vary as a function of land use (arable, orchards, and grassland), plant life cycle (annual and perennial) and climatic zone. In summary, this study shows that overall organic farming enhances total microbial abundance and activity in agricultural soils on a global scale. PMID:28700609

  12. Development of FAST.Farm: A New Multiphysics Engineering Tool for Wind Farm Design and Analysis: Preprint

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Jonkman, Jason; Annoni, Jennifer; Hayman, Greg

    2017-01-01

    This paper presents the development of FAST.Farm, a new multiphysics tool applicable to engineering problems in research and industry involving wind farm performance and cost optimization that is needed to address the current underperformance, failures, and expenses plaguing the wind industry. Achieving wind cost-of-energy targets - which requires improvements in wind farm performance and reliability, together with reduced uncertainty and expenditures - has been eluded by the complicated nature of the wind farm design problem, especially the sophisticated interaction between atmospheric phenomena and wake dynamics and array effects. FAST.Farm aims to balance the need for accurate modeling of the relevantmore » physics for predicting power performance and loads while maintaining low computational cost to support a highly iterative and probabilistic design process and system-wide optimization. FAST.Farm makes use of FAST to model the aero-hydro-servo-elastics of distinct turbines in the wind farm, and it is based on some of the principles of the Dynamic Wake Meandering (DWM) model, but avoids many of the limitations of existing DWM implementations.« less

  13. 12 CFR 619.9145 - Farm Credit Bank.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 6 2010-01-01 2010-01-01 false Farm Credit Bank. 619.9145 Section 619.9145 Banks and Banking FARM CREDIT ADMINISTRATION FARM CREDIT SYSTEM DEFINITIONS § 619.9145 Farm Credit Bank. The term Farm Credit Bank refers to a bank resulting from the mandatory merger of the Federal land...

  14. 12 CFR 619.9145 - Farm Credit Bank.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 12 Banks and Banking 6 2011-01-01 2011-01-01 false Farm Credit Bank. 619.9145 Section 619.9145 Banks and Banking FARM CREDIT ADMINISTRATION FARM CREDIT SYSTEM DEFINITIONS § 619.9145 Farm Credit Bank. The term Farm Credit Bank refers to a bank resulting from the mandatory merger of the Federal land...

  15. Comparisons of management practices and farm design on Australian commercial layer and meat chicken farms: Cage, barn and free range.

    PubMed

    Scott, Angela Bullanday; Singh, Mini; Toribio, Jenny-Ann; Hernandez-Jover, Marta; Barnes, Belinda; Glass, Kathryn; Moloney, Barbara; Lee, Amanda; Groves, Peter

    2017-01-01

    There are few published studies describing the unique management practices, farm design and housing characteristics of commercial meat chicken and layer farms in Australia. In particular, there has been a large expansion of free range poultry production in Australia in recent years, but limited information about this enterprise exists. This study aimed to describe features of Australian commercial chicken farms, with particular interest in free range farms, by conducting on-farm interviews of 25 free range layer farms, nine cage layer farms, nine barn layer farms, six free range meat chicken farms and 15 barn meat chicken farms in the Sydney basin bioregion and South East Queensland. Comparisons between the different enterprises (cage, barn and free range) were explored, including stocking densities, depopulation procedures, environmental control methods and sources of information for farmers. Additional information collected for free range farms include range size, range characteristics and range access. The median number of chickens per shed was greatest in free range meat chicken farms (31,058), followed by barn meat chicken (20,817), free range layer (10,713), barn layer (9,300) and cage layer farms (9,000). Sheds had cooling pads and tunnel ventilation in just over half of both barn and free range meat chicken farms (53%, n = 8) and was least common in free range layer farms (16%, n = 4). Range access in free range meat chicken farms was from sunrise to dark in the majority (93%, n = 14) of free range meat chicken farms. Over half of free range layer farms (56%, n = 14) granted range access at a set time each morning; most commonly between 9:00 to 10.00am (86%, n = 12), and chickens were placed back inside sheds when it was dusk.

  16. Comparisons of management practices and farm design on Australian commercial layer and meat chicken farms: Cage, barn and free range

    PubMed Central

    Singh, Mini; Toribio, Jenny-Ann; Hernandez-Jover, Marta; Barnes, Belinda; Glass, Kathryn; Moloney, Barbara; Lee, Amanda; Groves, Peter

    2017-01-01

    There are few published studies describing the unique management practices, farm design and housing characteristics of commercial meat chicken and layer farms in Australia. In particular, there has been a large expansion of free range poultry production in Australia in recent years, but limited information about this enterprise exists. This study aimed to describe features of Australian commercial chicken farms, with particular interest in free range farms, by conducting on-farm interviews of 25 free range layer farms, nine cage layer farms, nine barn layer farms, six free range meat chicken farms and 15 barn meat chicken farms in the Sydney basin bioregion and South East Queensland. Comparisons between the different enterprises (cage, barn and free range) were explored, including stocking densities, depopulation procedures, environmental control methods and sources of information for farmers. Additional information collected for free range farms include range size, range characteristics and range access. The median number of chickens per shed was greatest in free range meat chicken farms (31,058), followed by barn meat chicken (20,817), free range layer (10,713), barn layer (9,300) and cage layer farms (9,000). Sheds had cooling pads and tunnel ventilation in just over half of both barn and free range meat chicken farms (53%, n = 8) and was least common in free range layer farms (16%, n = 4). Range access in free range meat chicken farms was from sunrise to dark in the majority (93%, n = 14) of free range meat chicken farms. Over half of free range layer farms (56%, n = 14) granted range access at a set time each morning; most commonly between 9:00 to 10.00am (86%, n = 12), and chickens were placed back inside sheds when it was dusk. PMID:29166389

  17. 29 CFR 780.206 - Planting and lawn mowing.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... on that farm. (b) On the other hand, the planting of trees and bushes on residential, business, or... mowing. (a) The planting of trees and bushes is within the scope of agriculture where it constitutes a... of such trees or bushes). Thus, employees of the nurseryman who raised such nursery stock are doing...

  18. 29 CFR 780.206 - Planting and lawn mowing.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... on that farm. (b) On the other hand, the planting of trees and bushes on residential, business, or... mowing. (a) The planting of trees and bushes is within the scope of agriculture where it constitutes a... of such trees or bushes). Thus, employees of the nurseryman who raised such nursery stock are doing...

  19. 29 CFR 780.206 - Planting and lawn mowing.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... on that farm. (b) On the other hand, the planting of trees and bushes on residential, business, or... mowing. (a) The planting of trees and bushes is within the scope of agriculture where it constitutes a... of such trees or bushes). Thus, employees of the nurseryman who raised such nursery stock are doing...

  20. 29 CFR 780.206 - Planting and lawn mowing.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... on that farm. (b) On the other hand, the planting of trees and bushes on residential, business, or... mowing. (a) The planting of trees and bushes is within the scope of agriculture where it constitutes a... of such trees or bushes). Thus, employees of the nurseryman who raised such nursery stock are doing...

  1. 78 FR 7387 - Continuation of Farm Service Agency 2008 Farm Bill Programs

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-01

    ..., Conservation, and Energy Act of 2008 (the 2008 Farm Bill) through the 2013 crop year, fiscal year (FY), or... conservation programs administered by the Farm Service Agency (FSA). This notice provides information about... eligibility requirements, commodity programs, and conservation programs previously authorized or amended in...

  2. Orchid bees as bio-indicators for organic coffee farms in Costa Rica: does farm size affect their abundance?

    PubMed

    Hedström, Ingemar; Denzel, Andrew; Owens, Gareth

    2006-09-01

    The potential of Euglossini bees, especially Euglossa, as biological indicators of organic vs nonorganic coffee farms was studied in Atenas and San Isidro, Alajuela, Costa Rica using 1.8-cineole as lure. Observations were made for three days at each of four farms and complemented with data from a year of observations. Orchid bees were in greater abundance in the organic farms (t-Student test). However, lower abundances suggest that an organic farm may be negatively affected by the proximity of non-organic farms, depending on its size and distance. Orchid bees may be indicators of organic coffee farms.

  3. Effect of MeJA treatment on polyamine, energy status and anthracnose rot of loquat fruit.

    PubMed

    Cao, Shifeng; Cai, Yuting; Yang, Zhenfeng; Joyce, Daryl C; Zheng, Yonghua

    2014-02-15

    The effect of methyl jasmonate (MeJA) on changes in polyamines content and energy status and their relation to disease resistance was investigated. Freshly harvested loquat fruit were treated with 10 μmol l(-1) MeJA and wound inoculated with Colletotrichum acutatum spore suspension (1.0 × 10(5) spores ml(-1)) after 24h, and then stored at 20 °C for 6 days. MeJA treatment significantly reduced decay incidence. MeJA treated fruit manifested higher contents of polyamines (putrescine, spermidine and spermine) compared with the control fruit, during storage. MeJA treatment also maintained higher levels of adenosine triphosphate, and suppressed an increase in adenosine monophosphate content in loquat fruit. These results suggest that MeJA treatment may inhibit anthracnose rot by increasing polyamine content and maintaining the energy status. Copyright © 2013. Published by Elsevier Ltd.

  4. Farm Viability: Results of the USDA Family Farm Surveys. Rural Development Research Report No. 60.

    ERIC Educational Resources Information Center

    Salant, Priscilla; And Others

    The financial well-being of farm households in 29 Mississippi and Tennessee counties in 1980 and 8 Wisconsin counties in 1982 were analyzed. More than 90 percent of families planning to leave farming were viable under economic conditions at survey time. Almost half of Wisconsin dairy farm households were not viable. Higher debt, younger operator…

  5. Disease Interactions in a Shared Host Plant: Effects of Pre-Existing Viral Infection on Cucurbit Plant Defense Responses and Resistance to Bacterial Wilt Disease

    PubMed Central

    Mauck, Kerry E.; Pulido, Hannier; De Moraes, Consuelo M.; Stephenson, Andrew G.; Mescher, Mark C.

    2013-01-01

    Both biotic and abiotic stressors can elicit broad-spectrum plant resistance against subsequent pathogen challenges. However, we currently have little understanding of how such effects influence broader aspects of disease ecology and epidemiology in natural environments where plants interact with multiple antagonists simultaneously. In previous work, we have shown that healthy wild gourd plants (Cucurbita pepo ssp. texana) contract a fatal bacterial wilt infection (caused by Erwinia tracheiphila) at significantly higher rates than plants infected with Zucchini yellow mosaic virus (ZYMV). We recently reported evidence that this pattern is explained, at least in part, by reduced visitation of ZYMV-infected plants by the cucumber beetle vectors of E. tracheiphila. Here we examine whether ZYMV-infection may also directly elicit plant resistance to subsequent E. tracheiphila infection. In laboratory studies, we assayed the induction of key phytohormones (SA and JA) in single and mixed infections of these pathogens, as well as in response to the feeding of A. vittatum cucumber beetles on healthy and infected plants. We also tracked the incidence and progression of wilt disease symptoms in plants with prior ZYMV infections. Our results indicate that ZYMV-infection slightly delays the progression of wilt symptoms, but does not significantly reduce E. tracheiphila infection success. This observation supports the hypothesis that reduced rates of wilt disease in ZYMV-infected plants reflect reduced visitation by beetle vectors. We also documented consistently strong SA responses to ZYMV infection, but limited responses to E. tracheiphila in the absence of ZYMV, suggesting that the latter pathogen may effectively evade or suppress plant defenses, although we observed no evidence of antagonistic cross-talk between SA and JA signaling pathways. We did, however, document effects of E. tracheiphila on induced responses to herbivory that may influence host-plant quality for (and

  6. Urban farming model in South Jakarta

    NASA Astrophysics Data System (ADS)

    Indrawati, E.

    2018-01-01

    The development of infrastructure rapidly, large of population and large of urbanization. Meanwhile, agricultural land is decreasing and agricultural production continues to decline. The productive crops is needed for consumption and it is also to improve the environment from oxygen provisioning, antidote to air pollution and to improve soil conditions. The use of yard land for horticultural crops (vegetables, fruits and ornamental plants), spices, medicines, herbs etc. can benefit for the owners of the yard particularly and the general public. The purpose of this research is to identify the model of home yard utilization, mosque yard, office, school, urban park and main road and sub main road, which can improve environmental quality in Pesanggrahan district. The method of analysis used descriptive analysis method by observation. Then analyzed the percentage of the use of yard with productive crops as urban farming. The results showed that the most productive crops were planted in Kelurahan Pesanggrahan 67% which compared with in Kelurahan Ulujami 47%, and in Kelurahan Petukangan Utara 27%. The most types of productive crops were grown as fruit trees and vegetable crops.

  7. Farm accidents in children.

    PubMed Central

    Cameron, D.; Bishop, C.; Sibert, J. R.

    1992-01-01

    OBJECTIVE--To examine the problem of accidental injury to children on farms. DESIGN--Prospective county based study of children presenting to accident and emergency departments over 12 months with injuries sustained in a farm setting and nationwide review of fatal childhood farm accidents over the four years April 1986 to March 1990. SETTING--Accident and emergency departments in Aberystwyth, Carmarthen, Haverfordwest, and Llanelli and fatal accidents in England, Scotland, and Wales notified to the Health and Safety Executive register. SUBJECTS--Children aged under 16. MAIN OUTCOME MEASURE--Death or injury after farm related accidents. RESULTS--65 accidents were recorded, including 18 fractures. Nine accidents necessitated admission to hospital for a mean of two (range one to four) days. 13 incidents were related to tractors and other machinery; 24 were due to falls. None of these incidents were reported under the statutory notification scheme. 33 deaths were notified, eight related to tractors and allied machinery and 10 related to falling objects. CONCLUSIONS--Although safety is improving, the farm remains a dangerous environment for children. Enforcement of existing safety legislation with significant penalties and targeting of safety education will help reduce accident rates further. PMID:1638192

  8. Impact of a diversion of sewage effluent on a seaweed farm in the Seto Inland Sea, Japan

    NASA Astrophysics Data System (ADS)

    Zhang, X.; Uchiyama, Y.; Suzue, Y.

    2016-12-01

    The Seto Inland Sea (SIS), Japan, is a partially stratified, tidally driven estuary, comprising several semi-enclosed basins with complex coastlines and thousands of islands as well as substantial influences from the Kuroshio. Even though water pollution has been improved adequately because of related policies enacted, Osaka Bay (OB), a part of SIS, still suffers mostly due to sewage effluent resulted from the densely populated hinterland. Tarumi Sewage Treatment Plant (TSTP) is one of the largest wastewater treatment plants in OB, located near Akashi Strait (AS) where energetic and complex tidal flow occurs. The surrounding area is famous for a seaweed farming industry, while the local fishermen keep claiming possible impacts of TSTP effluent on a farm. Thus a new outfall was constructed away from the farm as a remedy, although its effect has not been extensively investigated yet. Therefore, a numerical modeling is required to assess the present situation and to further utilize it for improvement of the outfall design. In the present study, we develop a quadruple-nested high-resolution ocean model based on ROMS. The sewage effluent capability is implemented into the innermost ROMS-L4 model with horizontal grid spacing of 20 m as an additional Eulerian passive tracer model based on Uchiyama et al. (2014). Non-dimensional concentration of sewage effluent is applied at the locations of the two existing outfalls as a bottom-released freshwater plume at a constant volume rate of 180,000 m3/day. The normal sewage discharge results in eastward transport with frequent intrusions into the seaweed farm to the east of TSTP. The diversion discharge from the new outfall evidently alters salinity and tracer concentration in the farm owing to counter-clockwise residual circulation formed near AS that promotes southward (offshore) transport. The eastward effluent transport is reduced significantly by about 50 % on and around the eastern shore including the farm.

  9. Anti-inflammatory and antioxidant activity of polyphenolic extracts from Lactuca sativa (var. Maravilla de Verano) under different farming methods.

    PubMed

    Adesso, Simona; Pepe, Giacomo; Sommella, Eduardo; Manfra, Michele; Scopa, Antonio; Sofo, Adriano; Tenore, Gian Carlo; Russo, Mariateresa; Di Gaudio, Francesca; Autore, Giuseppina; Campiglia, Pietro; Marzocco, Stefania

    2016-09-01

    Besides their nutritional value, vegetables are a source of health-promoting compounds, such as polyphenols, and their content can be influenced by the particular farming method. In this study polyphenolic extracts from Lactuca sativa (var. Maravilla de verano) plants cultivated with different farming methods were chemically characterised and tested in vitro and ex vivo inflammation models. The tested extacts (250-2.5 µg mL(-1) ) were able to reduce both the inflammatory and oxidative stress in LPS-stimulated J774A.1 murine monocyte macrophage cells, by lowering the release of nitric oxide (NO) and reactive oxygen species (ROS) and promoting nuclear translocation of nuclear factor (erythroid-derived 2)-like 2; (Nrf2) and nuclear factor-κB (NF-κB). In this regard, quantitative profiles revealed different amounts of polyphenols, in particular quercetin levels were higher in plants under mineral fertilised treatment. Those extract showed an enhanced anti-inflammatory and antioxidant activity. Our data showed the anti-inflammatory and antioxidant potential of Maravilla de Verano polyphenolic extracts. The effect of farming methods on polyphenolic levels was highlighted. The higher reduction of inflammatory mediators release in extracts from plants cultivated under mineral fertilisation treatment was correlated to the higher amount of quercetin. These results can be useful for both nutraceutical or agronomic purposes. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry.

  10. Farm Family Adaptations to Severe Economic Distress: Wisconsin. Results of the 1989 Regional Farm Survey.

    ERIC Educational Resources Information Center

    Saupe, William E.; Eisenhauer, Janet

    During the farm crisis of the 1980s, many midwestern farm families suffered financial distress, but by 1989 an uneven financial recovery was under way. This report summarizes data collected from 622 Wisconsin farm operators (a 39% response rate) and 525 spouses as part of a large survey conducted in 12 North Central states. The purpose of the…

  11. [Nitrogen balance in dairy farm: research progress].

    PubMed

    Lü, Chao; Qin, Wen-Xiao; Gao, Teng-Yun; Wang, Xiao-Xiao; Han, Zhi-Guo; Li, Jia

    2013-01-01

    Large dairy farm with intensive management has high stocking density, but generally does not have enough space and normative feces disposal system, resulting in the discharged nitrogen surpassed the environmental carrying capacity of unit area land. Dairy farm is one of the major emission sources of nitrogen discharges in agriculture, where the nitrogen balance has being aroused attention by the experts abroad. The research on the nitrogen flow and nitrogen balance in dairy farm is the basis of the dairy farm nitrogen cycling and management study, as well as the basis for the construction of environmental laws, regulations and policies. The most reliable indicators to evaluate the nitrogen flow and nitrogen balance in dairy farm are nitrogen surplus and nitrogen use efficiency. This paper introduced the concept of nitrogen balance on farm-scale and the nitrogen flow within farm, compared the application scope of nitrogen surplus and nitrogen use efficiency, analyzed the factors affecting the nitrogen balance in dairy farm, and summarized the effective strategies to reduce the nitrogen discharges from dairy farm, aimed to provide references for the nitrogen management of dairy farm in China.

  12. Multiple phytohormone signalling pathways modulate susceptibility of tomato plants to Alternaria alternata f. sp. lycopersici

    PubMed Central

    Jia, Chengguo; Zhang, Liping; Wang, Qiaomei

    2013-01-01

    Three phytohormone molecules – ethylene (ET), jasmonic acid (JA) and salicylic acid (SA) – play key roles in mediating disease response to necrotrophic fungal pathogens. This study investigated the roles of the ET, JA, and SA pathways as well as their crosstalk during the interaction between tomato (Solanum lycopersicum) plants and a necrotrophic fungal pathogen Alternaria alternata f. sp. lycopersici (AAL). Both the ET and JASMONIC ACID INSENSITIVE1 (JAI1) receptor-dependent JA signalling pathways are necessary for susceptibility, while SA response promotes resistance to AAL infection. In addition, the role of JA in susceptibility to AAL is partly dependent on ET biosynthesis and perception, while the SA pathway enhances resistance to AAL and antagonizes the ET response. Based on these results, it is proposed that ET, JA, and SA each on their own can influence the susceptibility of tomato to AAL. Furthermore, the functions of JA and SA in susceptibility to the pathogen are correlated with the enhanced or decreased action of ET, respectively. This study has revealed the functional relationship among the three key hormone pathways in tomato defence against AAL. PMID:23264518

  13. Escherichia coli O157:H7 in beef cattle: on farm contamination and pre-slaughter control methods.

    PubMed

    Soon, J M; Chadd, S A; Baines, R N

    2011-12-01

    This paper addresses food safety in beef cattle production, with particular emphasis on factors that affect the prevalence of Escherichia coli O157:H7 in beef cattle and on control methods that have been investigated. Product recalls and foodborne diseases due to this organism continue to occur even though control measures have been under investigation for over 20 years. Most meatborne outbreaks are due to improper food handling practices and consumption of undercooked meat. However, the majority of pathogenic bacteria that can spread at slaughter by cross-contamination can be traced back to the farm rather than originating from the slaughter plant. This would ideally require the adoption of rigorous on-farm intervention strategies to mitigate risks at the farm level. On-farm strategies to control and reduce E. coli O157:H7 at the farm level will reduce the risk of carcass contamination at slaughter and processing facilities although they will not eliminate E. coli O157:H7. The most successful strategy for reducing the risk of contamination of beef and beef products will involve the implementation of both pre- and post-harvest measures.

  14. From axenic spore germination to molecular farming. One century of bryophyte in vitro culture.

    PubMed

    Hohe, Annette; Reski, Ralf

    2005-01-01

    The first bryophyte tissue culture techniques were established almost a century ago. All of the techniques that have been developed for tissue culture of seed plants have also been adapted for bryophytes, and these range from mere axenic culture to molecular farming. However, specific characteristics of bryophyte biology--for example, a unique regeneration capacity--have also resulted in the development of methodologies and techniques different than those used for seed plants. In this review we provide an overview of the application of in vitro techniques to bryophytes, emphasising the differences as well as the similarities between bryophytes and seed plants. These are discussed within the framework of physiological and developmental processes as well as with respect to potential applications in plant biotechnology.

  15. 12 CFR 619.9140 - Farm Credit bank(s).

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 6 2010-01-01 2010-01-01 false Farm Credit bank(s). 619.9140 Section 619.9140 Banks and Banking FARM CREDIT ADMINISTRATION FARM CREDIT SYSTEM DEFINITIONS § 619.9140 Farm Credit bank(s). Except as otherwise defined, the term Farm Credit bank(s) includes Farm Credit Banks...

  16. 12 CFR 619.9140 - Farm Credit bank(s).

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 12 Banks and Banking 6 2011-01-01 2011-01-01 false Farm Credit bank(s). 619.9140 Section 619.9140 Banks and Banking FARM CREDIT ADMINISTRATION FARM CREDIT SYSTEM DEFINITIONS § 619.9140 Farm Credit bank(s). Except as otherwise defined, the term Farm Credit bank(s) includes Farm Credit Banks...

  17. Proposal of optical farming: development of several optical sensing instruments for agricultural use

    NASA Astrophysics Data System (ADS)

    Saito, Y.; Kobayashi, K.

    2013-05-01

    We propose the use of "Optical Farming," which is the leading application of all types of optical technologies, in agriculture and agriculture-related industries. This paper focuses on the optical sensing instruments named "Agriserver," "Agrigadget" and "LIFS Monitor" developed in our laboratory. They are considered major factors in utilizing Optical Farming. Agriserver is a sensor network system that uses the Internet to collect information on agricultural products growing in fields. Agrigadget contains several optical devices, such as a smartphone-based spectroscopic device and a hand framing camera. LIFS Monitor is an advanced monitoring instrument that makes it possible to obtain physiological information of living plants. They are strongly associated with information communication technology. Their field and data usage performance in agricultural industries are reported.

  18. Farm Family Adaptations to Severe Economic Distress: Ohio. Results of the 1989 Regional Farm Survey.

    ERIC Educational Resources Information Center

    Lobao, Linda; Meyer, Katherine

    During the farm crisis of the 1980s, many midwestern farm families suffered financial distress, but by 1989 an uneven financial recovery was under way. This report summarizes data collected from 388 Ohio farm operators (a 38.8% response rate) and 353 spouses as part of a large survey conducted in 12 North Central states. The purpose of the survey…

  19. Farm level risk factors for fluoroquinolone resistance in E. coli and thermophilic Campylobacter spp. on poultry farms.

    PubMed

    Taylor, N M; Wales, A D; Ridley, A M; Davies, R H

    2016-10-01

    Data on husbandry practices, performance, disease and drug use were collected during a cross-sectional survey of 89 poultry meat farms in England and Wales to provide information on possible risk factors for the occurrence of fluoroquinolone (FQ)-resistant bacteria. Faeces samples were used to classify farms as "affected" or "not affected" by FQ-resistant (FQr) Escherichia coli or Campylobacter spp. Risk factor analysis identified the use of FQ on the farms as having by far the strongest association, among the factors considered, with the occurrence of FQr bacteria. Resistant E. coli and/or Campylobacter spp. were found on 86% of the farms with a history of FQ use. However, a substantial proportion of farms with no history of FQ use also yielded FQr organisms, suggesting that resistant bacteria may transfer between farms. Further analysis suggested that for Campylobacter spp., on-farm hygiene, cleaning and disinfection between batches of birds and wildlife control were of most significance. By contrast, for E. coli biosecurity from external contamination was of particular importance, although the modelling indicated that other factors were likely to be involved. Detailed studies on a small number of sites showed that FQr E. coli can survive routine cleaning and disinfection. It appears difficult to avoid the occurrence of resistant bacteria when FQ are used on a farm, but the present findings provide evidence to support recommendations to reduce the substantial risk of the incidental acquisition of such resistance by farms where FQ are not used.

  20. Elevated O3 enhances the attraction of whitefly-infested tomato plants to Encarsia formosa

    PubMed Central

    Cui, Hongying; Su, Jianwei; Wei, Jianing; Hu, Yongjian; Ge, Feng

    2014-01-01

    We experimentally examined the effects of elevated O3 and whitefly herbivory on tomato volatiles, feeding and oviposition preferences of whiteflies and behavioural responses of Encarsia formosa to these emissions on two tomato genotypes, a wild-type (Wt) and a jasmonic acid (JA) defence-enhanced genotype (JA-OE, 35S). The O3 level and whitefly herbivory significantly increased the total amount of volatile organic compounds (VOCs), monoterpenes, green leaf volatiles (GLVs), and aldehyde volatiles produced by tomato plants. The 35S plants released higher amount of total VOCs and monoterpene volatiles than Wt plants under O3+herbivory treatments. The feeding and oviposition bioassays showed that control plants were preferred by adult whiteflies whereas the 35S plants were not preferred by whiteflies. In the Y-tube tests, O3+herbivory treatment genotypes were preferred by adult E. Formosa. The 35S plants were preferred by adult E. formosa under O3, herbivory and O3+herbivory treatments. Our results demonstrated that elevated O3 and whitefly herbivory significantly increased tomato volatiles, which attracted E. formosa and reduced whitefly feeding. The 35S plants had a higher resistance to B. tabaci than Wt plant. Such changes suggest that the direct and indirect defences of resistant genotypes, such as 35S, could strengthen as the atmospheric O3 concentration increases. PMID:24939561

  1. Plant growth promotion properties of bacterial strains isolated from the rhizosphere of the Jerusalem artichoke (Helianthus tuberosus L.) adapted to saline-alkaline soils and their effect on wheat growth.

    PubMed

    Liu, Xiaolin; Li, Xiangyue; Li, Yan; Li, Runzhi; Xie, Zhihong

    2017-03-01

    The Jerusalem artichoke (JA; Helianthus tuberosus), known to be tolerant to saline-alkaline soil conditions, has been cultivated for many years in the Yellow River delta, Shandong Province coastal zone, in China. The aim of our study was to isolate nitrogen-fixing bacteria colonizing the rhizosphere of JA and to characterize other plant growth promotion properties. The ultimate goal was to identify isolates that could be used as inoculants benefiting an economic crop, in particular for improving wheat growth production in the Yellow River delta. Bacterial strains were isolated from the rhizosphere soil of JA on the basis of growth on nitrogen-free Ashby medium. Identification and phylogenetic analysis was performed after nucleotide sequencing of 16S rRNA gene. Plant-growth-promoting traits, such as nitrogen fixation activity, phosphate solubilization activity, indole-3-acetic acid production, were determined using conventional methods. Eleven strains were isolated and 6 of them were further examined for their level of salt tolerance and their effect on plant growth promotion. Inoculation of Enterobacter sp. strain N10 on JA and wheat led to significant increases in both root and shoot dry mass and shoot height. Enterobacter sp. strain N10 appeared to be the best plant-growth-promoting rhizobacteria to increase wheat productivity in future field applications.

  2. The phytoremediation potential of native plants on New Zealand dairy farms.

    PubMed

    Hahner, Jason L; Robinson, Brett H; Hong-Tao, Zhong; Dickinson, Nicholas M

    2014-01-01

    Ecological restoration of marginal land and riparian zones in agricultural landscapes in New Zealand enhances the provision of above-ground ecosystem services. We investigated whether native endemic plant assemblages have remediation potential, through modifying soil nutrient and trace element mobility. Analysis of native plant foliage in situ indicated that selective uptake of a range of commonly deficient trace elements including Zn, B, Cu, Mn and Co could provide a browse crop to avoid deficiencies of these elements in livestock, although some native plants may enhance the risk of Mo and Cd toxicity. Native plant rhizospheres were found to modify soil physico-chemistry and are likely to influence lateral and vertical fluxes of chemical elements in drainage waters. Native plants on marginal land in agricultural landscapes could add value to dairy production systems whilst helping to resolve topical environmental issues.

  3. METHODS FOR IMPROVEMENT OF TRICKLING FILTER PLANT PERFORMANCE. PART II. CHEMICAL ADDITION

    EPA Science Inventory

    An experimental program to explore potential methods for removing phosphorus and generally enhancing trickling filter plant performance was conducted at the Mason Farm Wastewater Treatment Plant, Chapel Hill, North Carolina. Preliminary investigations included jar testing with se...

  4. Effect of farming strategies on environmental impact of intensive dairy farms in Italy.

    PubMed

    Guerci, Matteo; Bava, Luciana; Zucali, Maddalena; Sandrucci, Anna; Penati, Chiara; Tamburini, Alberto

    2013-08-01

    Agriculture and animal husbandry are important contributors to global emissions of greenhouse (GHG) and acidifying gases. Moreover, they contribute to water pollution and to consumption of non-renewable natural resources such as land and energy. The Life Cycle Assessment (LCA) methodology allows evaluation of the environmental impact of a process from the production of inputs to the final product and to assess simultaneously several environmental impact categories among which GHG emissions, acidification, eutrophication, land use and energy use. The main purpose of this study was to evaluate, using the LCA methodology, the environmental impact of milk production in a sample of 41 intensive Italian dairy farms and to identify, among different farming strategies, those associated with the best environmental performances. The functional unit was 1 kg Fat and Protein Corrected Milk (FPCM). Farms showed characteristics of high production intensity: FPCM, expressed as tonnes per hectare, was 30·8±15·1. Total GHG emission per kg FPCM at farm gate was 1·30±0·19 kg CO2 eq. The main contributors to climate change potential were emissions from barns and manure storage (50·1%) and emissions for production and transportation of purchased feeds (21·2%). Average emission of gases causing acidification to produce 1 kg FPCM was 19·7±3·6 g of SO2 eq. Eutrophication potential was 9·01±1·78 ${\\rm PO}_{\\rm 4}^{{\\rm 3} -} {\\rm eq}.$ per kg FPCM on average. Farms from this study needed on average 5·97±1·32 MJ per kg FPCM from non-renewable energy sources. Energy consumption was mainly due to off-farm activities (58%) associated with purchased factors. Land use was 1·51±0·25 m2 per kg FPCM. The farming strategy based on high conversion efficiency at animal level was identified as the most effective to mitigate the environmental impact per kg milk at farm gate, especially in terms of GHG production and non-renewable energy use per kg FPCM.

  5. Synergistic effects of plant defense elicitors and Trichoderma harzianum on enhanced induction of antioxidant defense system in tomato against Fusarium wilt disease.

    PubMed

    Zehra, Andleeb; Meena, Mukesh; Dubey, Manish Kumar; Aamir, Mohd; Upadhyay, R S

    2017-11-02

    Plant defense against their pathogens can be induced by a complex network of different inducers. The present study investigates the synergistic effect of Trichoderma harzianum, exogenous salicylic acid (SA) and methyl jasmonate (MeJA) over the response and regulation of the antioxidant defense mechanisms and lipid peroxidation in tomato plants against Fusarium wilt disease. In the present work, tomato plants were infected by Fusarium oxysporum f. sp. lycopersici 3 days after inoculated with T. harzianum and/or sprayed daily for 3 days with chemical inducers (SA and MeJA). Plants were analysed at 0, 24, 48, 72 and 96 h after inoculation with Fusarium oxysporum f. sp. lycopersici. Infection of tomato plants by pathogen led to strong reduction in the dry weight of roots and shoots with the enhanced concentration of H 2 O 2 and varying degree of lipid peroxidation. Concurrently, exogenous SA, when applied with pathogen greatly enhanced H 2 O 2 content as well as activities of antioxidant enzymes except catalase (CAT) and ascorbate peroxidase (APx). The pathogen challenged plants pretreated with T. harzianum and MeJA together exhibited less lipid peroxidation and as well as the elevated level of ascorbic acid and enhanced activities of antioxidant enzymes. All applied treatments protected tomato seedlings against Fusarium wilt disease but the percentage of protection was found higher in plants pretreated with the combination of T. harzianum and chemical inducers.

  6. African swine fever outbreak on a medium-sized farm in Uganda: biosecurity breaches and within-farm virus contamination.

    PubMed

    Chenais, Erika; Sternberg-Lewerin, Susanna; Boqvist, Sofia; Liu, Lihong; LeBlanc, Neil; Aliro, Tonny; Masembe, Charles; Ståhl, Karl

    2017-02-01

    In Uganda, a low-income country in east Africa, African swine fever (ASF) is endemic with yearly outbreaks. In the prevailing smallholder subsistence farming systems, farm biosecurity is largely non-existent. Outbreaks of ASF, particularly in smallholder farms, often go unreported, creating significant epidemiological knowledge gaps. The continuous circulation of ASF in smallholder settings also creates biosecurity challenges for larger farms. In this study, an on-going outbreak of ASF in an endemic area was investigated on farm level, including analyses of on-farm environmental virus contamination. The study was carried out on a medium-sized pig farm with 35 adult pigs and 103 piglets or growers at the onset of the outbreak. Within 3 months, all pigs had died or were slaughtered. The study included interviews with farm representatives as well as biological and environmental sampling. ASF was confirmed by the presence of ASF virus (ASFV) genomic material in biological (blood, serum) and environmental (soil, water, feed, manure) samples by real-time PCR. The ASFV-positive biological samples confirmed the clinical assessment and were consistent with known virus characteristics. Most environmental samples were found to be positive. Assessment of farm biosecurity, interviews, and the results from the biological and environmental samples revealed that breaches and non-compliance with biosecurity protocols most likely led to the introduction and within-farm spread of the virus. The information derived from this study provides valuable insight regarding the implementation of biosecurity measures, particularly in endemic areas.

  7. On-Farm Diversity and Market Participation Are Positively Associated with Dietary Diversity of Rural Mothers in Southern Benin, West Africa

    PubMed Central

    Bellon, Mauricio R.; Ntandou-Bouzitou, Gervais D.; Caracciolo, Francesco

    2016-01-01

    Objective The objective of this study is to test the extent to which, under different opportunities for market participation, the diversity of plant species rural households grow or collect (on-farm diversity), and the variety of foods mothers purchase (market diversity) are associated with their dietary diversity. Methods Rural households from three districts in southern Benin were interviewed during dry (n = 472) and wet (n = 482) seasons between 2011 and 2012. Villages within districts and their households were selected randomly according to market accessibility, with a mother selected from each household. Information on on-farm diversity was collected using a semi-structured questionnaire. Market diversity was obtained through a 7-day food frequency questionnaire that elicited if foods were purchased. Dietary diversity was derived from a quantitative 24-hour food recall. A system of three simultaneous equations via a Generalized Methods of Moments was estimated to address potential endogeneity between dietary diversity and on-farm diversity and market diversity. Results Results show rich on-farm diversity with more than 65 different edible plant species grown or collected by households. More than 70% of foods consumed by mothers were purchased in 55 market places. More than 50% of mothers met minimum dietary diversity with at least 5 food groups consumed. Diagnostic tests indicated the existence of endogeneity. Econometric results showed that on-farm and market diversities were positively associated with mothers’ dietary diversity (p < 0.05) once market opportunities, seasonality and other socioeconomic factors were controlled for. Conclusion Results provide evidence of a positive relationship between on-farm diversity and dietary diversity among participant mothers. They demonstrate the important contribution of market diversity to their dietary diversity. Links among these three facets of diversity suggest that production for self-consumption and food

  8. On-Farm Diversity and Market Participation Are Positively Associated with Dietary Diversity of Rural Mothers in Southern Benin, West Africa.

    PubMed

    Bellon, Mauricio R; Ntandou-Bouzitou, Gervais D; Caracciolo, Francesco

    2016-01-01

    The objective of this study is to test the extent to which, under different opportunities for market participation, the diversity of plant species rural households grow or collect (on-farm diversity), and the variety of foods mothers purchase (market diversity) are associated with their dietary diversity. Rural households from three districts in southern Benin were interviewed during dry (n = 472) and wet (n = 482) seasons between 2011 and 2012. Villages within districts and their households were selected randomly according to market accessibility, with a mother selected from each household. Information on on-farm diversity was collected using a semi-structured questionnaire. Market diversity was obtained through a 7-day food frequency questionnaire that elicited if foods were purchased. Dietary diversity was derived from a quantitative 24-hour food recall. A system of three simultaneous equations via a Generalized Methods of Moments was estimated to address potential endogeneity between dietary diversity and on-farm diversity and market diversity. Results show rich on-farm diversity with more than 65 different edible plant species grown or collected by households. More than 70% of foods consumed by mothers were purchased in 55 market places. More than 50% of mothers met minimum dietary diversity with at least 5 food groups consumed. Diagnostic tests indicated the existence of endogeneity. Econometric results showed that on-farm and market diversities were positively associated with mothers' dietary diversity (p < 0.05) once market opportunities, seasonality and other socioeconomic factors were controlled for. Results provide evidence of a positive relationship between on-farm diversity and dietary diversity among participant mothers. They demonstrate the important contribution of market diversity to their dietary diversity. Links among these three facets of diversity suggest that production for self-consumption and food purchases complement rather than replace

  9. Prioritizing plant defence over growth through WRKY regulation facilitates infestation by non-target herbivores.

    PubMed

    Li, Ran; Zhang, Jin; Li, Jiancai; Zhou, Guoxin; Wang, Qi; Bian, Wenbo; Erb, Matthias; Lou, Yonggen

    2015-06-17

    Plants generally respond to herbivore attack by increasing resistance and decreasing growth. This prioritization is achieved through the regulation of phytohormonal signaling networks. However, it remains unknown how this prioritization affects resistance against non-target herbivores. In this study, we identify WRKY70 as a specific herbivore-induced, mitogen-activated protein kinase-regulated rice transcription factor that physically interacts with W-box motives and prioritizes defence over growth by positively regulating jasmonic acid (JA) and negatively regulating gibberellin (GA) biosynthesis upon attack by the chewing herbivore Chilo suppressalis. WRKY70-dependent JA biosynthesis is required for proteinase inhibitor activation and resistance against C. suppressalis. In contrast, WRKY70 induction increases plant susceptibility against the rice brown planthopper Nilaparvata lugens. Experiments with GA-deficient rice lines identify WRKY70-dependent GA signaling as the causal factor in N. lugens susceptibility. Our study shows that prioritizing defence over growth leads to a significant resistance trade-off with important implications for the evolution and agricultural exploitation of plant immunity.

  10. Persistence of Mycobacterium avium subsp. paratuberculosis at a Farm-Scale Biogas Plant Supplied with Manure from Paratuberculosis-Affected Dairy Cattle▿

    PubMed Central

    Slana, I.; Pribylova, R.; Kralova, A.; Pavlik, I.

    2011-01-01

    In this study, products from all steps of anaerobic digestion at a farm-scale biogas plant supplied with manure from paratuberculosis-affected dairy cattle were examined and quantified for the presence of the causal agent of paratuberculosis, Mycobacterium avium subsp. paratuberculosis, using culture and quantitative real-time PCR (qPCR). Viable M. avium subsp. paratuberculosis cells were detected using culture in fermentors for up to 2 months; the presence of M. avium subsp. paratuberculosis DNA (101 cells/g) was demonstrated in all anaerobic fermentors and digestate 16 months after initiation of work at a biogas plant, using IS900 qPCR. F57 qPCR was able to detect M. avium subsp. paratuberculosis DNA (102 cells/g) at up to 12 months. According to these results, a fermentation process that extended beyond 2 months removed all viable M. avium subsp. paratuberculosis cells and therefore rendered its product M. avium subsp. paratuberculosis free. However, M. avium subsp. paratuberculosis DNA was found during all the examined periods (more than 1 year), which could be explained by either residual DNA being released from dead cells or by the presence of viable cells whose amount was under the limit of cultivability. As the latter hypothesis cannot be excluded, the safety of the final products of digestion used for fertilization or animal bedding cannot be defined, and further investigation is necessary to confirm or refute this risk. PMID:21398476

  11. 12 CFR 614.4000 - Farm Credit Banks.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... institutions. (3) Farm Credit Banks, in their capacity as certified agricultural mortgage marketing facilities... 12 Banks and Banking 6 2010-01-01 2010-01-01 false Farm Credit Banks. 614.4000 Section 614.4000 Banks and Banking FARM CREDIT ADMINISTRATION FARM CREDIT SYSTEM LOAN POLICIES AND OPERATIONS Lending...

  12. Nitric oxide and gene regulation in plants.

    PubMed

    Grün, S; Lindermayr, C; Sell, S; Durner, J

    2006-01-01

    There is increasing evidence that nitric oxide (NO), which was first identified as a unique diffusible molecular messenger in animals, plays an important role in diverse physiological processes in plants. Recent progress that has deepened our understanding of NO signalling functions in plants, with special emphasis on defence signalling, is discussed here. Several studies, based on plants with altered NO-levels, have recently provided genetic evidence for the importance of NO in gene induction. For a general overview of which gene expression levels are altered by NO, two studies, involving large-scale transcriptional analyses of Arabidopsis thaliana using custom-made or commercial DNA-microarrays, were performed. Furthermore, a comprehensive transcript profiling by cDNA-amplification fragment length polymorphism (AFLP) revealed a number of Arabidopsis thaliana genes that are involved in signal transduction, disease resistance and stress response, photosynthesis, cellular transport, and basic metabolism. In addition, NO affects the expression of numerous genes in other plant species such as tobacco or soybean. The NO-dependent intracellular signalling pathway(s) that lead to the activation or suppression of these genes have not yet been defined. Several lines of evidence point to an interrelationship between NO and salicylic acid (SA) in plant defence. Recent evidence suggests that NO also plays a role in the wounding/jasmonic acid (JA) signalling pathway. NO donors affect both wounding-induced H2O2 synthesis and wounding- or JA-induced expression of defence genes. One of the major challenges ahead is to determine how the correct specific response is evoked, despite shared use of the NO signal and, in some cases, its downstream second messengers.

  13. Soil Decomposition of Added Organic C in an Organic Farming System

    NASA Astrophysics Data System (ADS)

    Kpomblekou-A, Kokoasse; Sissoko, Alassane; McElhenney, Wendell

    2015-04-01

    In the United States, large quantities of poultry waste are added every year to soil under organic management. Decomposition of the added organic C releases plant nutrients, promotes soil structure, and plays a vital role in the soil food web. In organic agriculture the added C serves as the only source of nutrients for plant growth. Thus understanding the decomposition rates of such C in organic farming systems are critical in making recommendations of organic inputs to organic producers. We investigated and compared relative accumulation and decomposition of organic C in an organic farming system trial at the George Washington Carver Agricultural Experiment Station at Tuskegee, Alabama on a Marvyn sandy loam (fine-loamy, kaolinitic, thermic, Typic Kanhapludults) soil. The experimental design was a randomized complete block with four replicates and four treatments. The main plot (54' × 20') was split into three equal subplots to plant three sweet potato cultivars. The treatments included a weed (control with no cover crop, no fertilizer), crimson clover alone (CC), crimson clover plus broiler litter (BL), and crimson clover plus NPK mineral fertilizers (NPK). For five years, late in fall, the field was planted with crimson clover (Trifolium incarnatum L) that was cut with a mower and incorporated into soil the following spring. Moreover, broiler litter (4.65 Mg ha-1) or ammonium nitrate (150 kg N ha-1), triple super phosphate (120 kg P2O5 ha-1), and potassium chloride (160 kg K2O ha-1) were applied to the BL or the NPK plot and planted with sweet potato. Just before harvest, six soil samples were collected within the two middle rows of each sweet potato plot with an auger at incremental depths of 0-1, 1-2, 2-3, 3-5, 5-10, and 10-15 cm. Samples from each subplot and depth were composited and mixed in a plastic bag. The samples were sieved moist through a

  14. Spore Density Determines Infection Strategy by the Plant Pathogenic Fungus Plectosphaerella cucumerina1[OPEN

    PubMed Central

    2016-01-01

    Necrotrophic and biotrophic pathogens are resisted by different plant defenses. While necrotrophic pathogens are sensitive to jasmonic acid (JA)-dependent resistance, biotrophic pathogens are resisted by salicylic acid (SA)- and reactive oxygen species (ROS)-dependent resistance. Although many pathogens switch from biotrophy to necrotrophy during infection, little is known about the signals triggering this transition. This study is based on the observation that the early colonization pattern and symptom development by the ascomycete pathogen Plectosphaerella cucumerina (P. cucumerina) vary between inoculation methods. Using the Arabidopsis (Arabidopsis thaliana) defense response as a proxy for infection strategy, we examined whether P. cucumerina alternates between hemibiotrophic and necrotrophic lifestyles, depending on initial spore density and distribution on the leaf surface. Untargeted metabolome analysis revealed profound differences in metabolic defense signatures upon different inoculation methods. Quantification of JA and SA, marker gene expression, and cell death confirmed that infection from high spore densities activates JA-dependent defenses with excessive cell death, while infection from low spore densities induces SA-dependent defenses with lower levels of cell death. Phenotyping of Arabidopsis mutants in JA, SA, and ROS signaling confirmed that P. cucumerina is differentially resisted by JA- and SA/ROS-dependent defenses, depending on initial spore density and distribution on the leaf. Furthermore, in situ staining for early callose deposition at the infection sites revealed that necrotrophy by P. cucumerina is associated with elevated host defense. We conclude that P. cucumerina adapts to early-acting plant defenses by switching from a hemibiotrophic to a necrotrophic infection program, thereby gaining an advantage of immunity-related cell death in the host. PMID:26842622

  15. Public farms: hygiene and zoonotic agents.

    PubMed

    Heuvelink, A E; Valkenburgh, S M; Tilburg, J J H C; Van Heerwaarden, C; Zwartkruis-Nahuis, J T M; De Boer, E

    2007-10-01

    In three successive years, we visited petting farms (n=132), care farms (n=91), and farmyard campsites (n=84), respectively, and completed a standard questionnaire with the objective of determining the hygienic status of these farms and describing hygiene measures implemented to reduce the risk of transmission of zoonotic agents from the animals to humans. For at least 85% of the farms, the overall impression of hygiene was recorded as good. However, more attention must be paid to: informing visitors on hygiene and handwashing, provision of handwashing facilities, and a footwear cleaning facility. Examination of samples of freshly voided faeces resulted in the detection of Shiga toxin-producing Escherichia coli O157 and/or Salmonella spp. and/or Campylobacter spp. at almost two-thirds (64.9%) of the petting farms, and around half of the care farms (56.0%) and farmyard campsites (45.2%). These data reinforce the need for control measures for both public and private farms to reduce human exposure to livestock faeces and thus the risk of transmission of zoonotic diseases. Public awareness of the risk associated with handling animals or faecal material should be increased.

  16. Nut production in response to thinning and fertilization for planted walnut

    Treesearch

    Felix Ponder; Steve Rutledge; J.W. Van Sambeek

    2013-01-01

    Nut production from nursery-run black walnuts grown on 225 acres at the Hammons Products Company's Sho-Neff Black Walnut Farm in Stockton, MO, was evaluated from 1995 to 2010 to determine if nut production increased after thinning and fertilization in 2001. The farm consists of 11 upland and 10 bottomland plantings on sites ranging from unsuitable to well suited...

  17. Real time wind farm emulation using SimWindFarm toolbox

    NASA Astrophysics Data System (ADS)

    Topor, Marcel

    2016-06-01

    This paper presents a wind farm emulation solution using an open source Matlab/Simulink toolbox and the National Instruments cRIO platform. This work is based on the Aeolus SimWindFarm (SWF) toolbox models developed at Aalborg university, Denmark. Using the Matlab Simulink models developed in SWF, the modeling code can be exported to a real time model using the NI Veristand model framework and the resulting code is integrated as a hardware in the loop control on the NI 9068 platform.

  18. Green factory: plants as bioproduction platforms for recombinant proteins.

    PubMed

    Xu, Jianfeng; Dolan, Maureen C; Medrano, Giuliana; Cramer, Carole L; Weathers, Pamela J

    2012-01-01

    Molecular farming, long considered a promising strategy to produce valuable recombinant proteins not only for human and veterinary medicine, but also for agriculture and industry, now has some commercially available products. Various plant-based production platforms including whole-plants, aquatic plants, plant cell suspensions, and plant tissues (hairy roots) have been compared in terms of their advantages and limits. Effective recombinant strategies are summarized along with descriptions of scalable culture systems and examples of commercial progress and success. Copyright © 2011 Elsevier Inc. All rights reserved.

  19. Simulation of logistics to supply Corn Stover to the Ontario Power Generation (OPG) Plant in Lambton, Ontario

    DOE PAGES

    Khaleghi Hamedani, Hamid; Lau, Anthony K.; DeBruyn, Jake; ...

    2016-05-10

    The overall goal of this research is to investigate the logistics of agricultural biomass in Ontario, Canada using the Integrated Biomass Supply Analysis and Logistics Model (IBSAL). The supply of corn stover to the Ontario Power Generation (OPG) power plant in Lambton is simulated. This coal-fired power plant is currently not operating and there are no active plans by OPG to fuel it with biomass. Rather, this scenario is considered only to demonstrate the application of the IBSAL Model to this type of scenario. Here, five scenarios of delivering corn stover to the Lambton Generating Station (GS) power plant inmore » Lambton Ontario are modeled: (1) truck transport from field edge to OPG (base scenario); (2) farm to central storage located on the highway, then truck transport bales to OPG; (3) direct truck transport from farm (no-stacking) to OPG; (4) farm to a loading port on Lake Huron and from there on a barge to OPG; and (5) farm to a railhead and then to OPG by rail.« less

  20. Simulation of logistics to supply Corn Stover to the Ontario Power Generation (OPG) Plant in Lambton, Ontario

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Khaleghi Hamedani, Hamid; Lau, Anthony K.; DeBruyn, Jake

    The overall goal of this research is to investigate the logistics of agricultural biomass in Ontario, Canada using the Integrated Biomass Supply Analysis and Logistics Model (IBSAL). The supply of corn stover to the Ontario Power Generation (OPG) power plant in Lambton is simulated. This coal-fired power plant is currently not operating and there are no active plans by OPG to fuel it with biomass. Rather, this scenario is considered only to demonstrate the application of the IBSAL Model to this type of scenario. Here, five scenarios of delivering corn stover to the Lambton Generating Station (GS) power plant inmore » Lambton Ontario are modeled: (1) truck transport from field edge to OPG (base scenario); (2) farm to central storage located on the highway, then truck transport bales to OPG; (3) direct truck transport from farm (no-stacking) to OPG; (4) farm to a loading port on Lake Huron and from there on a barge to OPG; and (5) farm to a railhead and then to OPG by rail.« less

  1. 12 CFR 614.4000 - Farm Credit Banks.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 12 Banks and Banking 6 2011-01-01 2011-01-01 false Farm Credit Banks. 614.4000 Section 614.4000 Banks and Banking FARM CREDIT ADMINISTRATION FARM CREDIT SYSTEM LOAN POLICIES AND OPERATIONS Lending Authorities § 614.4000 Farm Credit Banks. (a) Long-term real estate lending. Except to the extent such...

  2. Crashes involving farm tractors and other farm vehicles/equipment in North Carolina 1995-1999

    DOT National Transportation Integrated Search

    2000-04-01

    Tables and figures are shown to depict a statistical representation of crashes involving farm tractors and other farm vehicles or equipment, in North Carolina. Information displayed includes crash frequency, crashes by county, accident severity, type...

  3. Farm Population of the United States: 1974. Current Population Reports, Farm Population.

    ERIC Educational Resources Information Center

    Banks, Vera J.; And Others

    Based on data derived primarily from the Current Population Survey of the Bureau of the Census, this statistical report presents demographic and labor force characteristics of the U.S. farm population and a comparison of selected characteristics of the farm and nonfarm population. Tabular data are presented as follows: (1) Population of the U.S.,…

  4. How to measure the agroecological performance of farming in order to assist with the transition process.

    PubMed

    Trabelsi, Meriam; Mandart, Elisabeth; Le Grusse, Philippe; Bord, Jean-Paul

    2016-01-01

    The use of plant protection products enables farmers to maximize economic performance and yields, but in return, the environment and human health can be greatly affected because of their toxicity. There are currently strong calls for farmers to reduce the use of these toxic products for the preservation of the environment and the human health, and it has become urgent to invest in more sustainable models that help reduce these risks. One possible solution is the transition toward agroecological production systems. These new systems must be beneficial economically, socially, and environmentally in terms of human health. There are many tools available, based on a range of indicators, for assessing the sustainability of agricultural systems on conventional farm holdings. These methods are little suitable to agroecological farms and do not measure the performance of agroecological transition farms. In this article, we therefore develop a model for the strategic definition, guidance, and assistance for a transition to agroecological practices, capable of assessing performance of this transition and simulating the consequences of possible changes. This model was built by coupling (i) a decision-support tool and a technico-economic simulator with (ii) a conceptual model built from the dynamics of agroecological practices. This tool is currently being tested in the framework of a Compte d'Affectation Spéciale pour le Développement Agricole et Rural (CASDAR) project (CASDAR: project launched in 2013 by the French Ministry of Agriculture, Food and Forestry, on the theme "collective mobilisation for agroecology," http://agriculture.gouv.fr/Appel-a-projets-CASDAR ) using data from farms, most of which are engaged in agroenvironmental process and reducing plant protection treatments since 2008.

  5. Stability analysis of offshore wind farm and marine current farm

    NASA Astrophysics Data System (ADS)

    Shawon, Mohammad Hasanuzzaman

    Renewable energy has been playing an important role to meet power demand and 'Green Energy' market is getting bigger platform all over the world in the last few years. Due to massive increase in the prices of fossil fuels along with global warming issues, energy harvesting from renewable energy sources has received considerable interest, nowadays, where extensive researches are going on to ensure optimum use of renewable sources. In order to meet the increasing demand of electricity and power, integration of renewable energy is getting highest priorities around the world. Wind is one of the most top growing renewable energy resources and wind power market penetration is expected to reach 3.35 percent by 2013 from its present market of about 240 GW. A wind energy system is the most environmental friendly, cost effective and safe among all renewable energy resources available. Another promising form of renewable energy is ocean energy which covers 70 % of the earth. Ocean energy can be tapped from waves, tides and thermal elements. Offshore Wind farm (OWF) has already become very popular for large scale wind power integration with the onshore grid. Recently, marine current farm (MCF) is also showing good potential to become mainstream energy sources and already successfully commissioned in United Kingdom. However, squirrel cage induction generator (SCIG) has the stability problem similar to synchronous generator especially during fault location to restore the electromagnetic torque. Series dynamic braking resistor (SDBR) has been known as a useful mean to stabilize fixed speed wind generator system. On the other hand, doubly fed induction generator (DFIG) has the capability of coupling the control of active and reactive power and to provide necessary reactive power demand during grid fault conditions. Series dynamic braking resistor (SDBR) can also be employed with DFIG to limit the rotor over current. An integration of wind and tidal energy represents a new

  6. Understanding growers' decisions to manage invasive pathogens at the farm level.

    PubMed

    Breukers, Annemarie; van Asseldonk, Marcel; Bremmer, Johan; Beekman, Volkert

    2012-06-01

    Globalization causes plant production systems to be increasingly threatened by invasive pests and pathogens. Much research is devoted to support management of these risks. Yet, the role of growers' perceptions and behavior in risk management has remained insufficiently analyzed. This article aims to fill this gap by addressing risk management of invasive pathogens from a sociopsychological perspective. An analytical framework based on the Theory of Planned Behavior was used to explain growers' decisions on voluntary risk management measures. Survey information from 303 Dutch horticultural growers was statistically analyzed, including regression and cluster analysis. It appeared that growers were generally willing to apply risk management measures, and that poor risk management was mainly due to perceived barriers, such as high costs and doubts regarding efficacy of management measures. The management measures applied varied considerably among growers, depending on production sector and farm-specific circumstances. Growers' risk perception was found to play a role in their risk management, although the causal relation remained unclear. These results underscore the need to apply a holistic perspective to farm level management of invasive pathogen risk, considering the entire package of management measures and accounting for sector- and farm-specific circumstances. Moreover, they demonstrate that invasive pathogen risk management can benefit from a multidisciplinary approach that incorporates growers' perceptions and behavior.

  7. The effect of feed demand on greenhouse gas emissions and farm profitability for organic and conventional dairy farms.

    PubMed

    Kiefer, Lukas; Menzel, Friederike; Bahrs, Enno

    2014-12-01

    The reduction of product-related greenhouse gas (GHG) emissions in milk production appears to be necessary. The reduction of emissions on an individual farm might be highly accepted by farm owners if it were accompanied by an increase in profitability. Using life cycle assessments to determine the product carbon footprints (PCF) and farm-level evaluations to record profitability, we explored opportunities for optimization based on analysis of 81 organic and conventional pasture-based dairy farms in southern Germany. The objective of the present study was to detect common determining factors for low PCF and high management incomes (MI) to achieve GHG reductions at the lowest possible operational cost. In our sample, organic farms, which performed economically better than conventional farms, produced PCF that were significantly higher than those produced by conventional farms [1.61 ± 0.29 vs. 1.45 ± 0.28 kg of CO₂ equivalents (CO₂eq) per kg of milk; means ± SD)]. A multiple linear regression analysis of the sample demonstrated that low feed demand per kilogram of milk, high grassland yield, and low forage area requirements per cow are the main factors that decrease PCF. These factors are also useful for improving a farm's profitability in principle. For organic farms, a reduction of feed demand of 100 g/kg of milk resulted in a PCF reduction of 105 g of CO₂eq/kg of milk and an increase in MI of approximately 2.1 euro cents (c)/kg of milk. For conventional farms, a decrease of feed demand of 100 g/kg of milk corresponded to a reduction in PCF of 117 g of CO₂eq/kg of milk and an increase in MI of approximately 3.1 c/kg of milk. Accordingly, farmers could achieve higher profits while reducing GHG emissions. Improved education and training of farmers and consultants regarding GHG mitigation and farm profitability appear to be the best methods of improving efficiency under traditional and organic farming practices.

  8. Kansas Farm and Ranch Management Project.

    ERIC Educational Resources Information Center

    Albracht, James, Ed.

    Thirty-four units of instruction are included in this core curriculum in farm management for postsecondary farm and ranch management programs. Units of instruction are divided into twelve instructional areas: (1) Introduction to Financial Management, (2) Farm Business Arrangement, (3) Credit Management, (4) Budgeting, (5) Record Keeping, (6)…

  9. Food for Survival: Diagnosing Crop Patterns to Secure Lower Threshold Food Security Levels in Farm Households of Burundi.

    PubMed

    Niragira, Sanctus; D'Haese, Marijke; D'Haese, Luc; Ndimubandi, Jean; Desiere, Sam; Buysse, Jeroen

    2015-06-01

    Burundi is one of the world's poorest countries, coming last in the Global Food Index (2013). Yet, a large majority of its population depends on agriculture. Most smallholder families do not produce enough to support their own families. To estimate the optimal crop mix and resources needed to provide the family with food containing sufficient energy, fat, and protein. This study uses mathematical programming to obtain the optimal crop mix that could maximize output given the constraints on production factor endowments and the need to feed the household. The model is calibrated with household-level data collected in 2010 in Ngozi Province in northern Burundi. Four models are developed, each representing a different farm type. The typology is based on 2007 data. Model predictions are compared with data collected during a revisit of the area in 2012. By producing a smaller number of crops and concentrating on those in which they have a comparative advantage, and trading produce and input with other farms, large and medium-sized farms can improve their productivity and hire extra workers to supplement family labor. Predictions of crops to be planted coincided to a high degree with those that farmers planted 2 years after our survey on newly acquired plots. Despite land scarcity, it is still possible for households that own land to find optimal crop combinations that can meet their minimal food security requirements while generating a certain level of income. Nearly landless households would benefit from the increased off-farm employment opportunities. With only 0.05 ha of land per capita, the annotation Nearly Landless is used to highlight the limited access to land observed in this farm category. © The Author(s) 2015.

  10. Wind Farm Recommendation Report

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    John Reisenauer

    On April 21, 2011, an Idaho National Laboratory (INL) Land Use Committee meeting was convened to develop a wind farm recommendation for the Executive Council and a list of proposed actions for proceeding with the recommendation. In terms of land use, the INL Land Use Committee unanimously agrees that Site 6 is the preferred location of the alternatives presented for an INL wind farm. However, further studies and resolution to questions raised (stated in this report) by the INL Land Use Committee are needed for the preferred location. Studies include, but are not limited to, wind viability (6 months), batsmore » (2 years), and the visual impact of the wind farm. In addition, cultural resource surveys and consultation (1 month) and the National Environmental Policy Act process (9 to 12 months) need to be completed. Furthermore, there is no documented evidence of developers expressing interest in constructing a small wind farm on INL, nor a specific list of expectations or concessions for which a developer might expect INL to cover the cost. To date, INL assumes the National Environmental Policy Act activities will be paid for by the Department of Energy and INL (the environmental assessment has only received partial funding). However, other concessions also may be expected by developers such as roads, fencing, power line installation, tie-ins to substations, annual maintenance, snow removal, access control, down-time, and remediation. These types of concessions have not been documented, as a request, from a developer and INL has not identified the short and long-term cost liabilities for such concessions should a developer expect INL to cover these costs. INL has not identified a go-no-go funding level or the priority this Wind Farm Project might have with respect to other nuclear-related projects, should the wind farm remain an unfunded mandate. The Land Use Committee recommends Legal be consulted to determine what, if any, liabilities exist with the Wind Farm

  11. Energy balance in olive oil farms: comparison of organic and conventional farming systems.

    NASA Astrophysics Data System (ADS)

    Moreno, Marta M.; Meco, Ramón; Moreno, Carmen

    2013-04-01

    The viability of an agricultural production system not only depends on the crop yields, but especially on the efficient use of available resources. However, the current agricultural systems depend heavily on non-renewable energy consumption in the form of fertilizers, fossil fuels, pesticides and machinery. In developed countries, the economic profitability of different productive systems is dependent on the granting of subsidies of diverse origin that affect both production factors (or inputs) and the final product (or output). Leaving such external aids, energy balance analysis reveals the real and most efficient form of management for each agroclimatic region, and is also directly related to the economic activity and the environmental state. In this work we compare the energy balance resulting from organic and conventional olive oil farms under the semi-arid conditions of Central Spain. The results indicate that the mean energy supplied to the organic farms was sensitively lower (about 30%) in comparison with the conventional management, and these differences were more pronounced for the biggest farms (> 15 ha). Mean energy outputs were about 20% lower in the organic system, although organic small farms (< 15 ha) resulted more productive than the conventional small ones. However, these lower outputs were compensated by the major market value obtained from the organic products. Chemical fertilizers and pesticides reached about 60% of the total energy inputs in conventional farming; in the organic farms, however, this ratio scarcely reached 25%. Human labor item only represented a very small amount of the total energy input in both cases (less than 1%). As conclusions, both management systems were efficient from an energy point of view. The value of the organic production should be focused on the environmental benefits it provides, which are not usually considered in the conventional management on not valuing the damage it produces to the environment. Organic

  12. Not Your Family Farm

    ERIC Educational Resources Information Center

    Tenopir, Carol; Baker, Gayle; Grogg, Jill E.

    2007-01-01

    The information industry continues to consolidate, just as agribusiness has consolidated and now dominates farming. Both the family farm and the small information company still exist but are becoming rarer in an age of mergers, acquisitions, and increased economies of scale. Small companies distinguish themselves by high quality, special themes,…

  13. Reducing Stress of Farm Men and Women.

    ERIC Educational Resources Information Center

    Keating, Norah C.

    1987-01-01

    Questioned 753 farm men and women to identify factors associated with stress in farm families. Results suggest that high mastery provides the best buffer against stress for both farm men and women. The task of family life educators is to help farm families augment their personal and social resources while managing high financial and work demands.…

  14. Organic Farming, Gender, and the Labor Process

    ERIC Educational Resources Information Center

    Hall, Alan; Mogyorody, Veronika

    2007-01-01

    This paper seeks to explain variations in gender participation in farm production and decision-making through an analysis of organic farm types, sizes, and orientations. Based on both survey and case study data, the analysis shows that female farmers on vegetable farms and mixed livestock/cash crop farms are more likely to be involved in farm…

  15. Offshore wind farm layout optimization

    NASA Astrophysics Data System (ADS)

    Elkinton, Christopher Neil

    Offshore wind energy technology is maturing in Europe and is poised to make a significant contribution to the U.S. energy production portfolio. Building on the knowledge the wind industry has gained to date, this dissertation investigates the influences of different site conditions on offshore wind farm micrositing---the layout of individual turbines within the boundaries of a wind farm. For offshore wind farms, these conditions include, among others, the wind and wave climates, water depths, and soil conditions at the site. An analysis tool has been developed that is capable of estimating the cost of energy (COE) from offshore wind farms. For this analysis, the COE has been divided into several modeled components: major costs (e.g. turbines, electrical interconnection, maintenance, etc.), energy production, and energy losses. By treating these component models as functions of site-dependent parameters, the analysis tool can investigate the influence of these parameters on the COE. Some parameters result in simultaneous increases of both energy and cost. In these cases, the analysis tool was used to determine the value of the parameter that yielded the lowest COE and, thus, the best balance of cost and energy. The models have been validated and generally compare favorably with existing offshore wind farm data. The analysis technique was then paired with optimization algorithms to form a tool with which to design offshore wind farm layouts for which the COE was minimized. Greedy heuristic and genetic optimization algorithms have been tuned and implemented. The use of these two algorithms in series has been shown to produce the best, most consistent solutions. The influences of site conditions on the COE have been studied further by applying the analysis and optimization tools to the initial design of a small offshore wind farm near the town of Hull, Massachusetts. The results of an initial full-site analysis and optimization were used to constrain the boundaries of

  16. The Farm as an Educative Tool in the Development of Place Attachments among Irish Farm Youth

    ERIC Educational Resources Information Center

    Cassidy, Anne

    2017-01-01

    This paper focuses on the educative role of the farm in the development of relationships between young people and the homeplace they grew up on. The paper is based on qualitative interviews with a cohort of 30 Irish university students (15 men and 15 women) brought up on Irish family farms who would not become full-time farmers. The farm acts as…

  17. Roles of plant hormones and anti-apoptosis genes during drought stress in rice (Oryza sativa L.).

    PubMed

    Ubaidillah, Mohammad; Safitri, Fika Ayu; Jo, Jun-Hyeon; Lee, Sang-Kyu; Hussain, Adil; Mun, Bong-Gyu; Chung, Il Kyung; Yun, Byung-Wook; Kim, Kyung-Min

    2016-12-01

    We previously identified the rice (Oryza sativa) senescence-associated gene OsSAP which encodes a highly conserved protein involved in anti-apoptotic activity. This novel Bax suppressor-related gene regulates tolerance to multiple stresses in yeast. Here, we show the effects of drought stress on leaf and root tissues of plants over-expressing OsSAP in relation to the levels of phytohormones, abscisic acid (ABA), jasmonic acid (JA), indole-3-carboxylic acid (ICA), gibberellic acid (GA 3 ), and zeatin. Results showed that rice plants over-expressing SAP were tolerant to drought stress compared to wild type and the plants over-expressing AtBI-1, which is a homolog of the human Bax inhibitor-1 in Arabidopsis. ABA and JA levels in OsSAP and AtBI-1 transgenic plants consistently increased up to at least 3 days after drought treatment, whereas lower GA 3 levels were recorded during early drought period. Comparison between control and transgenic plants overexpressing anti-apoptosis genes OsSAP and AtBI-1 resulted in different patterns of hormone levels, indicating that these genes are involved in the plant responses to drought stress and present an opportunity for further study on drought stress tolerance in rice and other plant species.

  18. Multi-time scale energy management of wind farms based on comprehensive evaluation technology

    NASA Astrophysics Data System (ADS)

    Xu, Y. P.; Huang, Y. H.; Liu, Z. J.; Wang, Y. F.; Li, Z. Y.; Guo, L.

    2017-11-01

    A novel energy management of wind farms is proposed in this paper. Firstly, a novel comprehensive evaluation system is proposed to quantify economic properties of each wind farm to make the energy management more economical and reasonable. Then, a combination of multi time-scale schedule method is proposed to develop a novel energy management. The day-ahead schedule optimizes unit commitment of thermal power generators. The intraday schedule is established to optimize power generation plan for all thermal power generating units, hydroelectric generating sets and wind power plants. At last, the power generation plan can be timely revised in the process of on-line schedule. The paper concludes with simulations conducted on a real provincial integrated energy system in northeast China. Simulation results have validated the proposed model and corresponding solving algorithms.

  19. Farm Population of the United States: 1973. Current Population Reports, Farm Population.

    ERIC Educational Resources Information Center

    Banks, Vera J.; And Others

    Selected characteristics of the United States' farm population for 1973 are presented. The farm population consists of all persons living in rural territory on places of: (1) 10 or more acres if as much as $50 worth of agricultural products were sold from the place in the reporting year and (2) under 10 acres if as much as $250 worth of…

  20. Missouri Small Farm Family Program. Revised.

    ERIC Educational Resources Information Center

    Enlow, George; And Others

    Records maintained by rural extension designees on the Missouri Small Farm Family Program, (initiated in 1972 by the cooperative extension service to help low income farm families learn to use available resources to improve their quality of life) provided data re: family characteristics, farm improvement progress, and improvement in the quality of…

  1. 38 CFR 21.126 - Farm cooperative course.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... rehabilitation services. The areas in which proficiency is to be established include: (1) Planning; (2) Producing; (3) Marketing; (4) Maintaining farm equipment; (5) Conserving farm resources; (6) Financing the farm...

  2. Household and farm transitions in environmental context

    PubMed Central

    Deane, Glenn D.; Gutmann, Myron P.

    2010-01-01

    Recent debate in the literature on population, environment, and land use questions the applicability of theory that patterns of farm extensification and intensification correspond to the life course of farmers and to the life cycle of farm families. This paper extends the debate to the agricultural development of the United States Great Plains region, using unique data from 1875 to 1930 that link families to farms over time in 25 environmentally diverse Kansas townships. Results of multilevel statistical modeling indicate that farmer’s age, household size, and household structure are simultaneously related to both the extent of farm operations and the intensity of land use, taking into account local environmental conditions and time trends as Kansas was settled and developed. These findings validate farm- and life cycle theories and offer support for intergenerational motivations for farm development that include both daughters and sons. Environmental variation in aridity was a key driver of farm structure. PMID:21643468

  3. Occupations and the Farm.

    ERIC Educational Resources Information Center

    Ewert-Krocker, Laurie

    2001-01-01

    Describes "occupation" as a Montessori term, which the Hershey Montessori Farm School, in Huntsburg, Ohio, has adopted for any task arising from the needs of the farm that then generates a scientific or historic study. Includes lists of occupations pursued during 2000-2001 and samples of record forms students used to manage their work.…

  4. Urban Farm Business Plan Handbook

    EPA Pesticide Factsheets

    The Urban Farm Business Plan Handbook (this document) provides guidance for developing a business plan for the startup and operation of an urban farm. It focuses on food and non-food related cultivated agriculture.

  5. Plant breeding and rural development in the United States.

    Treesearch

    KE Woeste; SB Blanche; KA Moldenhauer; CD Nelson

    2010-01-01

    Plant breeders contributed enormously to the agricultural and economic development of the United States. By improving the profitability of farming, plant breeders improved the economic condition of farmers and contributed to the growth and structure of rural communities. In the years since World War II, agriculture and the quality of rural life have been driven by...

  6. Comparison of heavy metal levels of farmed and escaped farmed rainbow trout and health risk assessment associated with their consumption.

    PubMed

    Varol, Memet; Sünbül, Muhammet Raşit

    2017-10-01

    In this study, levels of ten metals (arsenic, cadmium, cobalt, chromium, copper, iron, manganese, nickel, lead, and zinc) in muscles of farmed and escaped farmed rainbow trout (Oncorhynchus mykiss) in the Keban Dam Reservoir (Turkey) were determined. Also, human health risks associated with their consumption were assessed. Of ten metals, only Co and Fe levels in escaped rainbow trout were significantly higher than those in farmed rainbow trout. The metal levels in farmed and escaped rainbow trout were below the maximum permissible limits. The estimated daily intake (EDI) of each metal in both farmed and escaped farmed rainbow trout was much lower than the respective tolerable daily intake (TDI). The target hazard quotient (THQ) values for individual metal and the total THQ values for combined metals were lower than 1 in both farmed and escaped rainbow trout, indicating no health risk for humans. The cancer risk (CR) values estimated for inorganic As in both farmed and escaped rainbow trout indicated low carcinogenic risk to the consumers. According to the maximum allowable monthly consumption limits (CR mm) , adults may safely consume 24 meals of farmed rainbow trout per month or 39 meals of escaped rainbow trout per month, with minimal adverse carcinogenic and non-carcinogenic health effects. This study revealed that the risk from consuming farmed and escaped farmed rainbow trout in the Keban Dam Reservoir due to these trace elements is minimal.

  7. 75 FR 54961 - Final Supplemental Environmental Impact Statement, Single Nuclear Unit at the Bellefonte Plant...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-09

    ... facility, a wind farm, a methane- gas cofiring facility, and several small solar photovoltaic facilities... maintenance of select plant systems and other regulatory compliance activities. Major buildings and plant... the plant cooling towers and the reactor, auxiliary, control, turbine, office, and service buildings...

  8. Growing Vegetables. People on the Farm.

    ERIC Educational Resources Information Center

    Department of Agriculture, Washington, DC. Office of Governmental and Public Affairs.

    This booklet, one in a series about life on modern farms, describes farm operations and some activities in the lives of six vegetable farmers throughout the United States. The booklet visits the tomato growing of Carl Schneider and his partners and the lettuce growing farm of Norman Martella, both in California. It then includes brief accounts of…

  9. Short-term effects of fertility management under organic farming in Mediterranean region on soil properties and tomato production

    NASA Astrophysics Data System (ADS)

    Cavoski, Ivana; Chami, Ziad Al; Jarrar, Mohammad; Dumontet, Stefano; Mondelli, Donato

    2014-05-01

    In organic farming, plant production depends almost exclusively on nutrient deriving from the decomposition of exogenous organic matter in soil which is able to provide significant quantities of several important nutrients for the plant growth. However, in the soil the timing and amount of mineralization often does not coincide with crop nutritional need, making in-season fertilization necessary. The Regulation (EC) No 889/2008 on organic farming standards recognizes these needs and allows the use of a limited range fertilizers and soil conditioners (inputs) in order to meet nutritional needs of the plants and to achieve short term economically viable yield. Short-term open field experiment was conducted at the Mediterranean Agronomic Institute of Bari (MAIB) located in Apulia region (Southern Italy) in order study the effects of different fertilization scenarios based on equilibrated nutritional requirement on tomato (Lycopersicon esculentum Mill, cv. San Marzano) production efficiency and soil chemical properties. In soil dressing phase, three months before planting, biochar (BCH), organic fertilizers (OF), combined treatment (BCH+OF), cattle manure and vineyard wood compost (MVC), dairy wastes industry and vineyard wood compost (DVC) and unamended soil as control (CON) were established. In the pre-crop phase, organic and/or mineral fertilizers were incorporated into the previous treatments except CON and BCH in order to achieve balanced N, P and K application rates for tomato plants. Different fertilization scenarios significantly increased the yields over CON and BCH treatments, maintaining fruits quality. In short period of time, most of the soil parameters remained invariable, only available phosphorus significantly increased in the treatments which received organic fertilizers maybe due to the slight reduction in soil pH. However, such results are not surprising, if we consider the quantity of amendments and fertilizers applied in the experiment, as well as

  10. Prevalence of Salmonella in diverse environmental farm samples.

    PubMed

    Rodriguez, Andres; Pangloli, Philipus; Richards, Harold A; Mount, John R; Draughon, F Ann

    2006-11-01

    The development of suitable intervention strategies to control Salmonella populations at the farm level requires reliable data on the occurrence and prevalence of the pathogen. Previous studies on Salmonella prevalence have focused on acquiring data from specific farm types and/or selected regions. The purpose of this study was to evaluate the distribution of this pathogen across a variety of farm types and regions in order to generate comparative data from a diverse group of environmental samples. Farm samples (n = 2,496) were collected quarterly from 18 different farms across five states (Tennessee, North Carolina, Alabama, California, and Washington) over a 24-month period. The participating farms included beef and dairy cattle operations, swine production and farrowing facilities, and poultry farms (both broiler chicken and turkey). The samples were analyzed for the presence of Salmonella by means of the U.S. Food and Drug Administration's Bacteriological Analytical Manual methods optimized for farm samples. Salmonella isolates were characterized by automated riboprinting. Salmonella serovars were recovered from 4.7% of all samples. The majority of positive findings were isolated from swine farms (57.3%). The occurrence of Salmonella was lower on dairy farms (17.9%), poultry farms (16.2%), and beef cattle farms (8.5%). The most commonly isolated serovar was Salmonella Anatum (48.4%), which was isolated notably more frequently than the next most common Salmonella serovars, Arizonae (12.1%) and Javiana (8.8%). The results of this study suggest that significant reservoirs of Salmonella populations still exist on swine production facilities and to a lesser extent in other animal production facilities. Data showed that the surrounding farm environment could be an important source of contamination.

  11. Temporal dynamics of plant succession in abandoned field in Mediterranean mountain areas: farming terraces and sloping fields (Iberian System, Spain)

    NASA Astrophysics Data System (ADS)

    Nadal-Romero, Estela; Errea, Paz; Lasanta, Teodoro

    2017-04-01

    Cropland abandonment is an important problem in mountain areas worldwide. This process represents the change from an agricultural management to an abandoned land in which a complex plant succession process occurs, with important hydromorphological effects, and consequences in water resources availability and soil erosion. Literature indicates that plant succession depends on multiple natural factors (soil properties, topography, climate, lithology, and distance to natural covers…) and anthropogenic factors (age of abandonment, management of each field during the cultivation period and after the abandonment…). Despite the advances, much is unknown about the vegetation succession, due to the complexity of ecological and social conditions in which land abandonment occurs. Recently, it is shown that only local factors can explain the heterogeneity of the process (Burel and Baudry, 2002; Jouba and Alados, 2012). In this work, we analyze the diversity of vegetation cover in abandonment fields in Cameros Viejo (Iberian System, Spain), related to the different field patterns (terraces and sloping fields) and the age of abandonment. Agricultural lands were delimited using aerial photographs from 1956 and 1978. The current land cover was obtained from SIOSE (Information System of Land Occupation in Spain). According to our cartography, cultivated land occupied as much as 15,491 ha (39% of the area), remaining abandoned 14,505 ha by 1978. Farming terraces occupied 55.9% of the abandoned area, and 44.1% as sloping fields. On the other hand, our cartography highlights the complexity of current land cover of abandoned fields in a landscape matrix of scrubland. Our results suggest that ecological succession is faster in farming terraces than in sloping fields, mostly until scrubland phase is attained. They also suggest that current land cover is better explained by the physical conditions of each field than by the abandonment age. Acknowledgement This research was supported

  12. Corporate Reporting on Farm Animal Welfare: An Evaluation of Global Food Companies’ Discourse and Disclosures on Farm Animal Welfare

    PubMed Central

    Sullivan, Rory; Amos, Nicky; van de Weerd, Heleen A.

    2017-01-01

    Simple Summary Companies that produce or sell food products from farm animals can have a major influence on the lives and welfare of these animals. The Business Benchmark on Farm Animal Welfare (BBFAW) conducts an annual evaluation of the farm animal welfare-related disclosures of some of the world’s largest food companies. The programme looks at companies’ published policies and commitments and examines whether these might lead to actions that can improve animal welfare on farms. It also assesses whether companies show leadership in this field. The BBFAW found that, in 2012 and 2013, around 70% of companies acknowledged animal welfare as a business issue, and that, between 2012 and 2013, there was clear evidence of an increased level of disclosure on farm animal welfare awareness in the companies that were assessed. However, only 34% (2012) and 44% (2013) of companies had published comprehensive farm animal welfare policies, suggesting that many companies have yet to report on farm animal welfare as a business issue or disclose their approach to farm animal welfare to stakeholders and society. Abstract The views that food companies hold about their responsibilities for animal welfare can strongly influence the lives and welfare of farm animals. If a company’s commitment is translated into action, it can be a major driver of animal welfare. The Business Benchmark on Farm Animal Welfare (BBFAW) is an annual evaluation of farm animal welfare-related practices, reporting and performance of food companies. The framework evaluates how close, based on their disclosures, companies are to best practice in three areas: Management Commitment, Governance & Performance and Leadership & Innovation. The BBFAW analysed information published by 68 (2012) and 70 (2013) of the world’s largest food companies. Around 70% of companies acknowledged animal welfare as a business issue. Between 2012 and 2013, the mean BBFAW score increased significantly by 5% (p < 0.001, Wilcoxon

  13. Techno-economical Analysis of Rooftop Grid-connected PV Dairy Farms; Case Study of Urmia University Dairy Farm

    NASA Astrophysics Data System (ADS)

    Nikbakht, A. M.; Aste, N.; Sarnavi, H. J.; Leonforte, F.

    2017-08-01

    The global trends indicate a growing commitment to renewable energy development because of declining fossil fuels and environmental threats. Moreover, the global demographic growth coupled with rising demands for food has escalated the rate of energy consumption in food section. This study aims to investigate the techno-economic impacts of a grid-connected rooftop PV plan applied for a educational dairy farm in Urmia university, with total estimated annual electrical energy consumption of 18,283 kWh, located at the north west part of Iran. Based on the current feed-in tariff and tremendously low electricity price in agriculture section in Iran, the plants with size ranged from 14.4 to 19.7 kWp (initial investment ranged from 26,000 to 36,000 USD) would be satisfied economically.

  14. Occupational Injuries on Thoroughbred Horse Farms: A Description of Latino and Non-Latino Workers’ Experiences

    PubMed Central

    Swanberg, Jennifer E.; Clouser, Jessica M.; Westneat, Susan C.; Marsh, Mary W.; Reed, Deborah B.

    2013-01-01

    Animal production is a dangerous industry and increasingly reliant on a Latino workforce. Within animal production, little is known about the risks or the occupational hazards of working on farms involved in various aspects of thoroughbred horse breeding. Extant research suggests that horse workers are at risk of musculoskeletal and respiratory symptoms, kicks, and other injuries. However, limited known research has examined the experiences of the industry’s workers, including immigrant workers, despite their prominence and increased vulnerability. Using data collected from thoroughbred farm representatives via a phone-administered survey, a 2-hour face-to-face semi-structured interview, and farm injury logs, this article identifies and describes types of injuries experienced by workers (N = 284) and their surrounding circumstances. Results indicate that general injuries and musculoskeletal strains, sprains, and tears account for a majority of injuries among workers on thoroughbred farms. Upper limbs and extremities are most frequently injured, while direct contact with the horse accounted for over half of all injuries. No differences in the diagnoses or distribution of injury were found by ethnicity; however, Latinos were more often struck by or trampled by a horse while non-Latinos were more often injured by an insect or plant. Implications and opportunities for future research are discussed. PMID:24351785

  15. Farm-level risk factors for fluoroquinolone resistance in E. coli and thermophilic Campylobacter spp. on finisher pig farms.

    PubMed

    Taylor, N M; Clifton-Hadley, F A; Wales, A D; Ridley, A; Davies, R H

    2009-08-01

    Logistic regression, supported by other statistical analyses was used to explore the possible association of risk factors with the fluoroquinolone (FQ)-resistance status of 108 pig finisher farms in Great Britain. The farms were classified as 'affected' or 'not affected' by FQ-resistant E. coli or Campylobacter spp. on the basis of isolation of organisms from faecal samples on media containing 1 mg/l FQ. The use of FQ was the most important factor associated with finding resistant E. coli and/or Campylobacter, which were found on 79% (FQ-resistant E. coli) and 86% (FQ-resistant Campylobacter) of farms with a history of FQ use. However, resistant bacteria were also found on 19% (FQ-resistant E. coli) and 54% (FQ-resistant Campylobacter) of farms with no history of FQ use. For FQ-resistant E. coli, biosecurity measures may be protective and there was strong seasonal variation, with more farms found affected when sampled in the summer. For FQ-resistant Campylobacter, the buying-in of grower stock may increase risk and good on-farm hygiene may be protective. The findings suggest that resistant organisms, particularly Campylobacter, may spread between pig farms.

  16. Plant reproduction is altered by simulated herbicide drift to constructed plant communities.

    PubMed

    Olszyk, David; Pfleeger, Thomas; Shiroyama, Tamotsu; Blakeley-Smith, Matthew; Lee, E Henry; Plocher, Milton

    2017-10-01

    Herbicide drift may have unintended impacts on native vegetation, adversely affecting individual species and plant communities. To determine the potential ecological effects of herbicide drift, small plant community plots were constructed using 9 perennial species found in different Willamette Valley (OR, USA) grassland habitats. Studies were conducted at 2 Oregon State University (Corvallis, OR, USA) farms in 2 separate years, with single and combined treatments of 0.01 to 0.2× field application rates (f.a.r.) of 1119 g ha -1 for glyphosate (active ingredient [a.i.] of 830 g ha -1 acid glyphosate) and 560 g ha -1 a.i. for dicamba. Plant responses were percentage of cover, number of reproductive structures, mature and immature seed production, and vegetative biomass. Herbicide effects differed with species, year, and, to a lesser extent, farm. Generally, 0.1 to 0.2× f.a.r. of the herbicides were required to affect reproduction in Camassia leichtlinii, Elymus glaucus, Eriophyllum lanatum, Festuca idahoensis, Iris tenax, and Prunella vulgaris. Eriophyllum lanatum also had a significant increase in percentage of immature seed dry weight with 0.01× f.a.r. of dicamba or the combination of glyphosate plus dicamba. Other species showed similar trends, but fewer significant responses. These studies indicated potential effects of low levels of herbicides on reproduction of native plants, and demonstrated a protocol whereby species growing in a constructed plant community can be evaluated for ecological responses. Environ Toxicol Chem 2017;36:2799-2813. Published 2017 SETAC. This article is a US government work and, as such, is in the public domain in the United States of America. Published 2017 SETAC. This article is a US government work and, as such, is in the public domain in the United States of America.

  17. Pseudomonas syringae pv. tomato DC3000: a model pathogen for probing disease susceptibility and hormone signaling in plants.

    PubMed

    Xin, Xiu-Fang; He, Sheng Yang

    2013-01-01

    Since the early 1980s, various strains of the gram-negative bacterial pathogen Pseudomonas syringae have been used as models for understanding plant-bacterial interactions. In 1991, a P. syringae pathovar tomato (Pst) strain, DC3000, was reported to infect not only its natural host tomato but also Arabidopsis in the laboratory, a finding that spurred intensive efforts in the subsequent two decades to characterize the molecular mechanisms by which this strain causes disease in plants. Genomic analysis shows that Pst DC3000 carries a large repertoire of potential virulence factors, including proteinaceous effectors that are secreted through the type III secretion system and a polyketide phytotoxin called coronatine, which structurally mimics the plant hormone jasmonate (JA). Study of Pst DC3000 pathogenesis has not only provided several conceptual advances in understanding how a bacterial pathogen employs type III effectors to suppress plant immune responses and promote disease susceptibility but has also facilitated the discovery of the immune function of stomata and key components of JA signaling in plants. The concepts derived from the study of Pst DC3000 pathogenesis may prove useful in understanding pathogenesis mechanisms of other plant pathogens.

  18. But How Do You Grow Plants Without Dirt?

    ERIC Educational Resources Information Center

    Howells, Ronald F.

    1978-01-01

    Describes a class project on hydroponic farming (growing plants in water and in organic nutrients rather than dirt). Students formed a corporation to raise necessary funds and paid dividends from the proceeds earned selling the crop. (JMB)

  19. Handing down the Farm? The Increasing Uncertainty of Irrigated Farm Succession in Australia

    ERIC Educational Resources Information Center

    Wheeler, S.; Bjornlund, H.; Zuo, A.; Edwards, J.

    2012-01-01

    Farming is still primarily a family concern in Australia. Having a farm successor in place is important as it is associated with the likelihood of the current farmer adapting to external conditions and hence may have long-term implications for the structure and profitability of agriculture. We used current and historical surveys across a number of…

  20. Analysing wind farm efficiency on complex terrains

    NASA Astrophysics Data System (ADS)

    Castellani, Francesco; Astolfi, Davide; Terzi, Ludovico; Schaldemose Hansen, Kurt; Sanz Rodrigo, Javier

    2014-06-01

    Actual performances of onshore wind farms are deeply affected both by wake interactions and terrain complexity: therefore monitoring how the efficiency varies with the wind direction is a crucial task. Polar efficiency plot is therefore a useful tool for monitoring wind farm performances. The approach deserves careful discussion for onshore wind farms, where orography and layout commonly affect performance assessment. The present work deals with three modern wind farms, owned by Sorgenia Green, located on hilly terrains with slopes from gentle to rough. Further, onshore wind farm of Nprrekffir Enge has been analysed as a reference case: its layout is similar to offshore wind farms and the efficiency is mainly driven by wakes. It is shown and justified that terrain complexity imposes a novel and more consistent way for defining polar efficiency. Dependency of efficiency on wind direction, farm layout and orography is analysed and discussed. Effects of atmospheric stability have been also investigated through MERRA reanalysis data from NASA satellites. Monin-Obukhov Length has been used to discriminate climate regimes.

  1. The sesquiterpene botrydial produced by Botrytis cinerea induces the hypersensitive response on plant tissues and its action is modulated by salicylic acid and jasmonic acid signaling.

    PubMed

    Rossi, Franco Rubén; Gárriz, Andrés; Marina, María; Romero, Fernando Matías; Gonzalez, María Elisa; Collado, Isidro González; Pieckenstain, Fernando Luis

    2011-08-01

    Botrytis cinerea, as a necrotrophic fungus, kills host tissues and feeds on the remains. This fungus is able to induce the hypersensitive response (HR) on its hosts, thus taking advantage on the host's defense machinery for generating necrotic tissues. However, the identity of HR effectors produced by B. cinerea is not clear. The aim of this work was to determine whether botrydial, a phytotoxic sesquiterpene produced by B. cinerea, is able to induce the HR on plant hosts, using Arabidopsis thaliana as a model. Botrydial induced the expression of the HR marker HSR3, callose deposition, and the accumulation of reactive oxygen species and phenolic compounds. Botrydial also induced the expression of PR1 and PDF1.2, two pathogenesis-related proteins involved in defense responses regulated by salicylic acid (SA) and jasmonic acid (JA), respectively. A. thaliana and tobacco plants defective in SA signaling were more resistant to botrydial than wild-type plants, as opposed to A. thaliana plants defective in JA signaling, which were more sensitive. It can be concluded that botrydial induces the HR on its hosts and its effects are modulated by host signaling pathways mediated by SA and JA.

  2. 7 CFR 1412.47 - Planting flexibility.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Administrator, is prohibited on base acreage of a farm enrolled in a DCP or ACRE program contract. Harvesting... payments will not be reduced for the planting or harvesting of the fruit, vegetable, or wild rice; (2) The... root, lychee (litchi), mandarins, mangos, marionberries, mar bub, melongene, mesple, mizuna, mongosteen...

  3. 7 CFR 1412.47 - Planting flexibility.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... Administrator, is prohibited on base acreage of a farm enrolled in a DCP or ACRE program contract. Harvesting... payments will not be reduced for the planting or harvesting of the fruit, vegetable, or wild rice; (2) The... root, lychee (litchi), mandarins, mangos, marionberries, mar bub, melongene, mesple, mizuna, mongosteen...

  4. 7 CFR 1412.47 - Planting flexibility.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... Administrator, is prohibited on base acreage of a farm enrolled in a DCP or ACRE program contract. Harvesting... payments will not be reduced for the planting or harvesting of the fruit, vegetable, or wild rice; (2) The... root, lychee (litchi), mandarins, mangos, marionberries, mar bub, melongene, mesple, mizuna, mongosteen...

  5. 7 CFR 1412.47 - Planting flexibility.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... Administrator, is prohibited on base acreage of a farm enrolled in a DCP or ACRE program contract. Harvesting... payments will not be reduced for the planting or harvesting of the fruit, vegetable, or wild rice; (2) The... root, lychee (litchi), mandarins, mangos, marionberries, mar bub, melongene, mesple, mizuna, mongosteen...

  6. Simulations for Making On-farm Decisions in Relation to ENSO in Semi-arid Areas, South Africa

    NASA Astrophysics Data System (ADS)

    Tesfuhuney, W. A.; Crespo, O. O.; Walker, S. S.; Steyn, S. A.

    2017-12-01

    The study was employed to investigate and improve on-farm decision making on planting dates and fertilization by relating simulated yield and seasonal outlook information. The Agricultural Production Systems SIMulator model (APSIM) was used to explore ENSO/SOI effects for small-scale farmers to represent weather conditions and soil forms of semi-arid areas of Bothaville, Bethlehem and Bloemfontein regions in South Africa. The relationships of rainfall and SOI anomalies indicate a positive correlation, signifies ENSO/SOI as seasonal outlooks for study areas. Model evaluation results showed higher degree of bias (RMSEs/RMSE value of 0.88-0.98). The D-index of agreement in the range 0.61-0.71 indicate the ability of the APSIM-Maize model is an adequate tool in evaluating relative changes in maize yield in relation to various management practices and seasonal variations. During rainy, La Niño years (SOI > +5), highest simulated yields were found for Bethlehem in November with addition of 100 - 150 kg ha-1 N fertilization and up to 50 kg ha-1 for both Bothaville and Bloemfontein. With respect to various levels of fertilization, the dry El Niño years (SOI < -5) had a range of 0.90-1.31, 3.03-3.54 and 1.11-1.26 t ha-1 yields and showed to increase during La Niña years with a range of 2.50-2.66, 3.36-4.79 and 2.24-2.38 t ha-1 at Bothaville, Bethlehem and Bloemfontein for November planting. During El Niño episodes planting earlier and using 50 kg ha-1 fertilizer with improved short maturing cultivar are effective adaptation measures to counteract poor soils and erratic rainfall of semi-arid environment, Under optimal soil conditions and/or when probability of La Niño episodes, optimal yields are obtained by maximizing fertilization. Effective rainfall and tactical on-farm management decisions in associate with seasonal rainfall out looks information is a useful mechanism in reducing risk for dryland farming in semi-arid regions. Key word: Semi-arid; APSIM; SOI; El Ni

  7. A systems approach to assess farm-scale nutrient and trace element dynamics: a case study at the Ojebyn dairy farm.

    PubMed

    Oborn, Ingrid; Modin-Edman, Anna-Karin; Bengtsson, Helena; Gustafson, Gunnela M; Salomon, Eva; Nilsson, S Ingvar; Holmqvist, Johan; Jonsson, Simon; Sverdrup, Harald

    2005-06-01

    A systems analysis approach was used to assess farmscale nutrient and trace element sustainability by combining full-scale field experiments with specific studies of nutrient release from mineral weathering and trace-element cycling. At the Ojebyn dairy farm in northern Sweden, a farm-scale case study including phosphorus (P), potassium (K), and zinc (Zn) was run to compare organic and conventional agricultural management practices. By combining different element-balance approaches (at farmgate, barn, and field scales) and further adapting these to the FARMFLOW model, we were able to combine mass flows and pools within the subsystems and establish links between subsystems in order to make farm-scale predictions. It was found that internal element flows on the farm are large and that there are farm internal sources (Zn) and loss terms (K). The approaches developed and tested at the Ojebyn farm are promising and considered generally adaptable to any farm.

  8. Farm Business Management Analysis: Analyzing the Farm Business. Unit II. Volume 13, Number 7.

    ERIC Educational Resources Information Center

    Denker, Robert; And Others

    Intended for use by Missouri vocational agricultural instructors in Farm Business Management Analysis programs for young and adult farmers, this curriculum guide contains 10 lessons in analyzing records. Each lesson is a self-contained instructional package and includes materials for monthly classroom sessions and monthly on-the-farm instructional…

  9. Data Farming in Support of NATO

    DTIC Science & Technology

    2014-03-01

    What are your national/international experiences on data farming ? • Finland : Technology forecasting, indirect fire studies (19,000 different...from the military bringing their questions to the data farming community. • Which simulation systems have been used in the past? • Finland : SANDIS...sponsors. • Do you have standardized procedures to conduct a data farming experiment? • Finland : No standard procedure (ad hoc at the moment, still

  10. Is agritourism eco-friendly? A comparison between agritourisms and other farms in Italy using farm accountancy data network dataset.

    PubMed

    Mastronardi, Luigi; Giaccio, Vincenzo; Giannelli, Agostino; Scardera, Alfonso

    2015-01-01

    This paper presents the results of research regarding the environmental performances of Italian farms with agritourism compared with farms without agritourism. In Italy, agritourism is considered an agricultural activity and can only be performed by a farmer. Moreover, Italian national legislation forces the farmer to dedicate himself mainly to traditional farming, rather than to tourism activities. For this reason, environmental performances have been highlighted by analyzing only features and production systems of the farms. By utilizing the most frequent indicators used in studies regarding sustainability, the authors show how Italian agritourisms tend to develop more environmentally friendly agricultural methods, which have a positive impact on biodiversity, landscape and natural resources. The empirical analysis is based on the Italian FADN (Farm Accountancy Data Network) dataset. The European FADN was created to represent farms' technical and economic operation in the European Union and on which it drafts the agricultural and rural policies. The dichotomous structure of the dependent variable (presence or absence of agritourism at the farm) has a propensity for an assessment method based on Binary Response Model Regression.

  11. Molecular diversity of arbuscular mycorrhizal fungi in onion roots from organic and conventional farming systems in the Netherlands.

    PubMed

    Galván, Guillermo A; Parádi, István; Burger, Karin; Baar, Jacqueline; Kuyper, Thomas W; Scholten, Olga E; Kik, Chris

    2009-06-01

    Diversity and colonization levels of naturally occurring arbuscular mycorrhizal fungi (AMF) in onion roots were studied to compare organic and conventional farming systems in the Netherlands. In 2004, 20 onion fields were sampled in a balanced survey between farming systems and between two regions, namely, Zeeland and Flevoland. In 2005, nine conventional and ten organic fields were additionally surveyed in Flevoland. AMF phylotypes were identified by rDNA sequencing. All plants were colonized, with 60% for arbuscular colonization and 84% for hyphal colonization as grand means. In Zeeland, onion roots from organic fields had higher fractional colonization levels than those from conventional fields. Onion yields in conventional farming were positively correlated with colonization level. Overall, 14 AMF phylotypes were identified. The number of phylotypes per field ranged from one to six. Two phylotypes associated with the Glomus mosseae-coronatum and the G. caledonium-geosporum species complexes were the most abundant, whereas other phylotypes were infrequently found. Organic and conventional farming systems had similar number of phylotypes per field and Shannon diversity indices. A few organic and conventional fields had larger number of phylotypes, including phylotypes associated with the genera Glomus-B, Archaeospora, and Paraglomus. This suggests that farming systems as such did not influence AMF diversity, but rather specific environmental conditions or agricultural practices.

  12. Integrating nitric oxide into salicylic acid and jasmonic acid/ ethylene plant defense pathways.

    PubMed

    Mur, Luis A J; Prats, Elena; Pierre, Sandra; Hall, Michael A; Hebelstrup, Kim H

    2013-01-01

    Plant defense against pests and pathogens is known to be conferred by either salicylic acid (SA) or jasmonic acid (JA)/ethylene (ET) pathways, depending on infection or herbivore-grazing strategy. It is well attested that SA and JA/ET pathways are mutually antagonistic allowing defense responses to be tailored to particular biotic stresses. Nitric oxide (NO) has emerged as a major signal influencing resistance mediated by both signaling pathways but no attempt has been made to integrate NO into established SA/JA/ET interactions. NO has been shown to act as an inducer or suppressor of signaling along each pathway. NO will initiate SA biosynthesis and nitrosylate key cysteines on TGA-class transcription factors to aid in the initiation of SA-dependent gene expression. Against this, S-nitrosylation of NONEXPRESSOR OF PATHOGENESIS-RELATED PROTEINS1 (NPR1) will promote the NPR1 oligomerization within the cytoplasm to reduce TGA activation. In JA biosynthesis, NO will initiate the expression of JA biosynthetic enzymes, presumably to over-come any antagonistic effects of SA on JA-mediated transcription. NO will also initiate the expression of ET biosynthetic genes but a suppressive role is also observed in the S-nitrosylation and inhibition of S-adenosylmethionine transferases which provides methyl groups for ET production. Based on these data a model for NO action is proposed but we have also highlighted the need to understand when and how inductive and suppressive steps are used.

  13. Integrating nitric oxide into salicylic acid and jasmonic acid/ ethylene plant defense pathways

    PubMed Central

    Mur, Luis A. J.; Prats, Elena; Pierre, Sandra; Hall, Michael A.; Hebelstrup, Kim H.

    2013-01-01

    Plant defense against pests and pathogens is known to be conferred by either salicylic acid (SA) or jasmonic acid (JA)/ethylene (ET) pathways, depending on infection or herbivore-grazing strategy. It is well attested that SA and JA/ET pathways are mutually antagonistic allowing defense responses to be tailored to particular biotic stresses. Nitric oxide (NO) has emerged as a major signal influencing resistance mediated by both signaling pathways but no attempt has been made to integrate NO into established SA/JA/ET interactions. NO has been shown to act as an inducer or suppressor of signaling along each pathway. NO will initiate SA biosynthesis and nitrosylate key cysteines on TGA-class transcription factors to aid in the initiation of SA-dependent gene expression. Against this, S-nitrosylation of NONEXPRESSOR OF PATHOGENESIS-RELATED PROTEINS1 (NPR1) will promote the NPR1 oligomerization within the cytoplasm to reduce TGA activation. In JA biosynthesis, NO will initiate the expression of JA biosynthetic enzymes, presumably to over-come any antagonistic effects of SA on JA-mediated transcription. NO will also initiate the expression of ET biosynthetic genes but a suppressive role is also observed in the S-nitrosylation and inhibition of S-adenosylmethionine transferases which provides methyl groups for ET production. Based on these data a model for NO action is proposed but we have also highlighted the need to understand when and how inductive and suppressive steps are used. PMID:23818890

  14. Farm Operating and Financial Characteristics, 1985. Statistical Bulletin Number 762.

    ERIC Educational Resources Information Center

    Morehart, Mitchell J.

    This report contains 56 tables that provide an overview of the operating and financial characteristics of farms in the United States in 1985. The report provides information on farms and land in farms; farm acreage purchased, sold, and rented; farm labor and wages; capital investments and improvement; farm production expenses; farm and nonfarm…

  15. Interaction between Nitric Oxide and Ethylene in the Induction of Alternative Oxidase in Ozone-Treated Tobacco Plants1[W

    PubMed Central

    Ederli, Luisa; Morettini, Roberta; Borgogni, Andrea; Wasternack, Claus; Miersch, Otto; Reale, Lara; Ferranti, Francesco; Tosti, Nicola; Pasqualini, Stefania

    2006-01-01

    The higher plant mitochondrial electron transport chain contains, in addition to the cytochrome chain, an alternative pathway that terminates with a single homodimeric protein, the alternative oxidase (AOX). We recorded temporary inhibition of cytochrome capacity respiration and activation of AOX pathway capacity in tobacco plants (Nicotiana tabacum L. cv BelW3) fumigated with ozone (O3). The AOX1a gene was used as a molecular probe to investigate its regulation by signal molecules such as hydrogen peroxide, nitric oxide (NO), ethylene (ET), salicylic acid, and jasmonic acid (JA), all of them reported to be involved in the O3 response. Fumigation leads to accumulation of hydrogen peroxide in mitochondria and early accumulation of NO in leaf tissues. Although ET accumulation was high in leaf tissues 5 h after the start of O3 fumigation, it declined during the recovery period. There were no differences in the JA and 12-oxo-phytodienoic acid levels of treated and untreated plants. NO, JA, and ET induced AOX1a mRNA accumulation. Using pharmacological inhibition of ET and NO, we demonstrate that both NO- and ET-dependent pathways are required for O3-induced up-regulation of AOX1a. However, only NO is indispensable for the activation of AOX1a gene expression. PMID:16935990

  16. Prioritizing plant defence over growth through WRKY regulation facilitates infestation by non-target herbivores

    PubMed Central

    Li, Ran; Zhang, Jin; Li, Jiancai; Zhou, Guoxin; Wang, Qi; Bian, Wenbo; Erb, Matthias; Lou, Yonggen

    2015-01-01

    Plants generally respond to herbivore attack by increasing resistance and decreasing growth. This prioritization is achieved through the regulation of phytohormonal signaling networks. However, it remains unknown how this prioritization affects resistance against non-target herbivores. In this study, we identify WRKY70 as a specific herbivore-induced, mitogen-activated protein kinase-regulated rice transcription factor that physically interacts with W-box motifs and prioritizes defence over growth by positively regulating jasmonic acid (JA) and negatively regulating gibberellin (GA) biosynthesis upon attack by the chewing herbivore Chilo suppressalis. WRKY70-dependent JA biosynthesis is required for proteinase inhibitor activation and resistance against C. suppressalis. In contrast, WRKY70 induction increases plant susceptibility against the rice brown planthopper Nilaparvata lugens. Experiments with GA-deficient rice lines identify WRKY70-dependent GA signaling as the causal factor in N. lugens susceptibility. Our study shows that prioritizing defence over growth leads to a significant resistance trade-off with important implications for the evolution and agricultural exploitation of plant immunity. DOI: http://dx.doi.org/10.7554/eLife.04805.001 PMID:26083713

  17. Organic farming increases richness of fungal taxa in the wheat phyllosphere.

    PubMed

    Karlsson, Ida; Friberg, Hanna; Kolseth, Anna-Karin; Steinberg, Christian; Persson, Paula

    2017-07-01

    Organic farming is often advocated as an approach to mitigate biodiversity loss on agricultural land. The phyllosphere provides a habitat for diverse fungal communities that are important for plant health and productivity. However, it is still unknown how organic farming affects the diversity of phyllosphere fungi in major crops. We sampled wheat leaves from 22 organically and conventionally cultivated fields in Sweden, paired based on their geographical location and wheat cultivar. Fungal communities were described using amplicon sequencing and real-time PCR. Species richness was higher on wheat leaves from organically managed fields, with a mean of 54 operational taxonomic units (OTUs) compared with 40 OTUs for conventionally managed fields. The main components of the fungal community were similar throughout the 350-km-long sampling area, and seven OTUs were present in all fields: Zymoseptoria, Dioszegia fristingensis, Cladosporium, Dioszegia hungarica, Cryptococcus, Ascochyta and Dioszegia. Fungal abundance was highly variable between fields, 10 3 -10 5 internal transcribed spacer copies per ng wheat DNA, but did not differ between cropping systems. Further analyses showed that weed biomass was the strongest explanatory variable for fungal community composition and OTU richness. These findings help provide a more comprehensive understanding of the effect of organic farming on the diversity of organism groups in different habitats within the agroecosystem. © 2017 The Authors Molecular Ecology Published by John Wiley & Sons Ltd.

  18. Conducting On-Farm Animal Research: Procedures & Economic Analysis.

    ERIC Educational Resources Information Center

    Amir, Pervaiz; Knipscheer, Hendrik C.

    This book is intended to give animal scientists elementary tools to perform on-farm livestock analysis and to provide crop-oriented farming systems researchers with methods for conducting animal research. Chapter 1 describes farming systems research as a systems approach to on-farm animal research. Chapter 2 outlines some important…

  19. Agricultural Adjustment and the Diversification of Farm Households and Corporate Farms in Central Europe

    ERIC Educational Resources Information Center

    Chaplin, Hannah; Davidova, Sophia; Gorton, Matthew

    2004-01-01

    Survey evidence from three Central European Countries (Czech Republic, Hungary and Poland) is analysed to identify the degree of non-agricultural farm diversification and the factors facilitating or impeding it in individual and corporate farms. The effect of diversification on rural job creation and household incomes is investigated. The results…

  20. Hexanoic acid is a resistance inducer that protects tomato plants against Pseudomonas syringae by priming the jasmonic acid and salicylic acid pathways.

    PubMed

    Scalschi, Loredana; Vicedo, Begonya; Camañes, Gemma; Fernandez-Crespo, Emma; Lapeña, Leonor; González-Bosch, Carmen; García-Agustín, Pilar

    2013-05-01

    Hexanoic acid-induced resistance (Hx-IR) is effective against several pathogens in tomato plants. Our study of the mechanisms implicated in Hx-IR against Pseudomonas syringae pv. tomato DC3000 suggests that hexanoic acid (Hx) treatment counteracts the negative effect of coronatine (COR) and jasmonyl-isoleucine (JA-Ile) on the salicylic acid (SA) pathway. In Hx-treated plants, an increase in the expression of jasmonic acid carboxyl methyltransferase (JMT) and the SA marker genes PR1 and PR5 indicates a boost in this signalling pathway at the expense of a decrease in JA-Ile. Moreover, Hx treatment potentiates 12-oxo-phytodienoic acid accumulation, which suggests that this molecule might play a role per se in Hx-IR. These results support a positive relationship between the SA and JA pathways in Hx-primed plants. Furthermore, one of the mechanisms of virulence mediated by COR is stomatal re-opening on infection with P. syringae. In this work, we observed that Hx seems to inhibit stomatal opening in planta in the presence of COR, which suggests that, on infection in tomato, this treatment suppresses effector action to prevent bacterial entry into the mesophyll. © 2012 BSPP AND BLACKWELL PUBLISHING LTD.

  1. Farm Animal Welfare and Human Health.

    PubMed

    Goldberg, Alan M

    2016-09-01

    The paper examines the relationship between farm animal welfare, industrial farm animal production, and human health consequences. The data suggest that when the animal welfare of land-based farm animals is compromised, there are resulting significant negative human health consequences due to environmental degradation, the use of non-therapeutic levels of antibiotics for growth promotion, and the consequences of intensification. This paper accepts that even if meat and fish consumption is reduced, meat and fish will be part of the diet of the future. Industrial production modified from the current intensified systems will still be required to feed the world in 2050 and beyond. This paper identifies the concept of sustainable intensification and suggests that if farm animal welfare is improved, many of the human health consequences of intensified industrial production can be eliminated or reduced. In water-based farm animal production, many new systems are resulting in a product that actually protects the environment and can be done at industrial levels without the use of antibiotics.

  2. Crop Farm Employee. Agricultural Cooperative Training. Vocational Agriculture. Revised.

    ERIC Educational Resources Information Center

    Boyd, Chester; And Others

    Designed for students enrolled in the Vocational Agricultural Cooperative Part-Time Training Program, this course of study contains 13 units for crop farm employees. Units include (examples of unit topics in parentheses): introduction (opportunities in farming, farming as a science, and farming in the United States), farm records (keeping farm…

  3. Yield and economic performance of organic and conventional cotton-based farming systems--results from a field trial in India.

    PubMed

    Forster, Dionys; Andres, Christian; Verma, Rajeev; Zundel, Christine; Messmer, Monika M; Mäder, Paul

    2013-01-01

    The debate on the relative benefits of conventional and organic farming systems has in recent time gained significant interest. So far, global agricultural development has focused on increased productivity rather than on a holistic natural resource management for food security. Thus, developing more sustainable farming practices on a large scale is of utmost importance. However, information concerning the performance of farming systems under organic and conventional management in tropical and subtropical regions is scarce. This study presents agronomic and economic data from the conversion phase (2007-2010) of a farming systems comparison trial on a Vertisol soil in Madhya Pradesh, central India. A cotton-soybean-wheat crop rotation under biodynamic, organic and conventional (with and without Bt cotton) management was investigated. We observed a significant yield gap between organic and conventional farming systems in the 1(st) crop cycle (cycle 1: 2007-2008) for cotton (-29%) and wheat (-27%), whereas in the 2(nd) crop cycle (cycle 2: 2009-2010) cotton and wheat yields were similar in all farming systems due to lower yields in the conventional systems. In contrast, organic soybean (a nitrogen fixing leguminous plant) yields were marginally lower than conventional yields (-1% in cycle 1, -11% in cycle 2). Averaged across all crops, conventional farming systems achieved significantly higher gross margins in cycle 1 (+29%), whereas in cycle 2 gross margins in organic farming systems were significantly higher (+25%) due to lower variable production costs but similar yields. Soybean gross margin was significantly higher in the organic system (+11%) across the four harvest years compared to the conventional systems. Our results suggest that organic soybean production is a viable option for smallholder farmers under the prevailing semi-arid conditions in India. Future research needs to elucidate the long-term productivity and profitability, particularly of cotton and

  4. 38 CFR 21.126 - Farm cooperative course.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 2 2013-07-01 2013-07-01 false Farm cooperative course.... Chapter 31 Educational and Vocational Training Services § 21.126 Farm cooperative course. (a) Definition. An approvable farm cooperative course is a full-time course designated to restore employability by...

  5. 38 CFR 21.126 - Farm cooperative course.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 2 2014-07-01 2014-07-01 false Farm cooperative course.... Chapter 31 Educational and Vocational Training Services § 21.126 Farm cooperative course. (a) Definition. An approvable farm cooperative course is a full-time course designated to restore employability by...

  6. 38 CFR 21.126 - Farm cooperative course.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 2 2011-07-01 2011-07-01 false Farm cooperative course.... Chapter 31 Educational and Vocational Training Services § 21.126 Farm cooperative course. (a) Definition. An approvable farm cooperative course is a full-time course designated to restore employability by...

  7. 38 CFR 21.126 - Farm cooperative course.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 2 2012-07-01 2012-07-01 false Farm cooperative course.... Chapter 31 Educational and Vocational Training Services § 21.126 Farm cooperative course. (a) Definition. An approvable farm cooperative course is a full-time course designated to restore employability by...

  8. Capturing farm diversity with hypothesis-based typologies: An innovative methodological framework for farming system typology development

    PubMed Central

    Alvarez, Stéphanie; Timler, Carl J.; Michalscheck, Mirja; Paas, Wim; Descheemaeker, Katrien; Tittonell, Pablo; Andersson, Jens A.; Groot, Jeroen C. J.

    2018-01-01

    Creating typologies is a way to summarize the large heterogeneity of smallholder farming systems into a few farm types. Various methods exist, commonly using statistical analysis, to create these typologies. We demonstrate that the methodological decisions on data collection, variable selection, data-reduction and clustering techniques can bear a large impact on the typology results. We illustrate the effects of analysing the diversity from different angles, using different typology objectives and different hypotheses, on typology creation by using an example from Zambia’s Eastern Province. Five separate typologies were created with principal component analysis (PCA) and hierarchical clustering analysis (HCA), based on three different expert-informed hypotheses. The greatest overlap between typologies was observed for the larger, wealthier farm types but for the remainder of the farms there were no clear overlaps between typologies. Based on these results, we argue that the typology development should be guided by a hypothesis on the local agriculture features and the drivers and mechanisms of differentiation among farming systems, such as biophysical and socio-economic conditions. That hypothesis is based both on the typology objective and on prior expert knowledge and theories of the farm diversity in the study area. We present a methodological framework that aims to integrate participatory and statistical methods for hypothesis-based typology construction. This is an iterative process whereby the results of the statistical analysis are compared with the reality of the target population as hypothesized by the local experts. Using a well-defined hypothesis and the presented methodological framework, which consolidates the hypothesis through local expert knowledge for the creation of typologies, warrants development of less subjective and more contextualized quantitative farm typologies. PMID:29763422

  9. Capturing farm diversity with hypothesis-based typologies: An innovative methodological framework for farming system typology development.

    PubMed

    Alvarez, Stéphanie; Timler, Carl J; Michalscheck, Mirja; Paas, Wim; Descheemaeker, Katrien; Tittonell, Pablo; Andersson, Jens A; Groot, Jeroen C J

    2018-01-01

    Creating typologies is a way to summarize the large heterogeneity of smallholder farming systems into a few farm types. Various methods exist, commonly using statistical analysis, to create these typologies. We demonstrate that the methodological decisions on data collection, variable selection, data-reduction and clustering techniques can bear a large impact on the typology results. We illustrate the effects of analysing the diversity from different angles, using different typology objectives and different hypotheses, on typology creation by using an example from Zambia's Eastern Province. Five separate typologies were created with principal component analysis (PCA) and hierarchical clustering analysis (HCA), based on three different expert-informed hypotheses. The greatest overlap between typologies was observed for the larger, wealthier farm types but for the remainder of the farms there were no clear overlaps between typologies. Based on these results, we argue that the typology development should be guided by a hypothesis on the local agriculture features and the drivers and mechanisms of differentiation among farming systems, such as biophysical and socio-economic conditions. That hypothesis is based both on the typology objective and on prior expert knowledge and theories of the farm diversity in the study area. We present a methodological framework that aims to integrate participatory and statistical methods for hypothesis-based typology construction. This is an iterative process whereby the results of the statistical analysis are compared with the reality of the target population as hypothesized by the local experts. Using a well-defined hypothesis and the presented methodological framework, which consolidates the hypothesis through local expert knowledge for the creation of typologies, warrants development of less subjective and more contextualized quantitative farm typologies.

  10. Plant Phenotyping using Probabilistic Topic Models: Uncovering the Hyperspectral Language of Plants

    PubMed Central

    Wahabzada, Mirwaes; Mahlein, Anne-Katrin; Bauckhage, Christian; Steiner, Ulrike; Oerke, Erich-Christian; Kersting, Kristian

    2016-01-01

    Modern phenotyping and plant disease detection methods, based on optical sensors and information technology, provide promising approaches to plant research and precision farming. In particular, hyperspectral imaging have been found to reveal physiological and structural characteristics in plants and to allow for tracking physiological dynamics due to environmental effects. In this work, we present an approach to plant phenotyping that integrates non-invasive sensors, computer vision, as well as data mining techniques and allows for monitoring how plants respond to stress. To uncover latent hyperspectral characteristics of diseased plants reliably and in an easy-to-understand way, we “wordify” the hyperspectral images, i.e., we turn the images into a corpus of text documents. Then, we apply probabilistic topic models, a well-established natural language processing technique that identifies content and topics of documents. Based on recent regularized topic models, we demonstrate that one can track automatically the development of three foliar diseases of barley. We also present a visualization of the topics that provides plant scientists an intuitive tool for hyperspectral imaging. In short, our analysis and visualization of characteristic topics found during symptom development and disease progress reveal the hyperspectral language of plant diseases. PMID:26957018

  11. The profitability of automatic milking on Dutch dairy farms.

    PubMed

    Bijl, R; Kooistra, S R; Hogeveen, H

    2007-01-01

    Several studies have reported on the profitability of automatic milking based on different simulation models, but a data-based study using actual farm data has been lacking. The objective of this study was to analyze the profitability of dairy farms having an automatic milking system (AMS) compared with farms using a conventional milking system (CMS) based on real accounting data. In total, 62 farms (31 using an AMS and 31 using a CMS) were analyzed for the year 2003 in a case control study. Differences between the years 2002 and 2003 also were analyzed by comparing a subgroup of 16 farms with an AMS and 16 farms with a CMS. Matching was based on the time of investment in a milking system (same year), the total milk production per year, and intensity of land use (kg/ha). Results from 2003 showed that the farms with an AMS used, on average, 29% less labor than farms with a CMS. In contrast, farms using a CMS grew faster (37,132 kg of milk quota and 5 dairy cows) than farms with an AMS (-3,756 kg milk quota and 0.5 dairy cows) between 2002 and 2003. Dairy farmers with a CMS had larger (euro7,899) revenues than those with an AMS. However, no difference in the margin on dairy production was detected, partly because of numerically greater (euro6,822) variable costs on CMS farms. Dairy farms were compared financially based on the amount of money that was available for rent, depreciation, interest, labor, and profit (RDILP). The CMS farms had more money (euro15,566) available for RDILP than the AMS farms. This difference was caused by larger fixed costs (excluding labor) for the AMS farms, larger contractor costs (euro6,422), and larger costs for gas, water, and electricity (euro1,549). Differences in costs for contractors and for gas, water, and electricity were statistically significant. When expressed per full-time employee, AMS farms had greater revenues, margins, and gross margins per full-time employee than did CMS farms. This resulted in a substantially greater

  12. Integrated watershed- and farm-scale modeling framework for targeting critical source areas while maintaining farm economic viability.

    PubMed

    Ghebremichael, Lula T; Veith, Tamie L; Hamlett, James M

    2013-01-15

    Quantitative risk assessments of pollution and data related to the effectiveness of mitigating best management practices (BMPs) are important aspects of nonpoint source pollution control efforts, particularly those driven by specific water quality objectives and by measurable improvement goals, such as the total maximum daily load (TMDL) requirements. Targeting critical source areas (CSAs) that generate disproportionately high pollutant loads within a watershed is a crucial step in successfully controlling nonpoint source pollution. The importance of watershed simulation models in assisting with the quantitative assessments of CSAs of pollution (relative to their magnitudes and extents) and of the effectiveness of associated BMPs has been well recognized. However, due to the distinct disconnect between the hydrological scale in which these models conduct their evaluation and the farm scale at which feasible BMPs are actually selected and implemented, and due to the difficulty and uncertainty involved in transferring watershed model data to farm fields, there are limited practical applications of these tools in the current nonpoint source pollution control efforts by conservation specialists for delineating CSAs and planning targeting measures. There are also limited approaches developed that can assess impacts of CSA-targeted BMPs on farm productivity and profitability together with the assessment of water quality improvements expected from applying these measures. This study developed a modeling framework that integrates farm economics and environmental aspects (such as identification and mitigation of CSAs) through joint use of watershed- and farm-scale models in a closed feedback loop. The integration of models in a closed feedback loop provides a way for environmental changes to be evaluated with regard to the impact on the practical aspects of farm management and economics, adjusted or reformulated as necessary, and revaluated with respect to effectiveness of

  13. Farm Wives' Labor Force Participation and Earnings.

    ERIC Educational Resources Information Center

    Godwin, Deborah D.; Marlowe, Julia

    1990-01-01

    Examines relationship between employment earnings and farm wives' decisions to work off-farm. Examines effects of wives' human capital, home factors, and labor market on work decisions and earnings. Education, experience, debt, and farm size were stronger influences on wives' decisions than on their earnings variations, once employed. (TES)

  14. Farm animal genomics and informatics: an update

    PubMed Central

    Fadiel, Ahmed; Anidi, Ifeanyi; Eichenbaum, Kenneth D.

    2005-01-01

    Farm animal genomics is of interest to a wide audience of researchers because of the utility derived from understanding how genomics and proteomics function in various organisms. Applications such as xenotransplantation, increased livestock productivity, bioengineering new materials, products and even fabrics are several reasons for thriving farm animal genome activity. Currently mined in rapidly growing data warehouses, completed genomes of chicken, fish and cows are available but are largely stored in decentralized data repositories. In this paper, we provide an informatics primer on farm animal bioinformatics and genome project resources which drive attention to the most recent advances in the field. We hope to provide individuals in biotechnology and in the farming industry with information on resources and updates concerning farm animal genome projects. PMID:16275782

  15. JaSTA-2: Second version of the Java Superposition T-matrix Application

    NASA Astrophysics Data System (ADS)

    Halder, Prithish; Das, Himadri Sekhar

    2017-12-01

    In this article, we announce the development of a new version of the Java Superposition T-matrix App (JaSTA-2), to study the light scattering properties of porous aggregate particles. It has been developed using Netbeans 7.1.2, which is a java integrated development environment (IDE). The JaSTA uses double precision superposition T-matrix codes for multi-sphere clusters in random orientation, developed by Mackowski and Mischenko (1996). The new version consists of two options as part of the input parameters: (i) single wavelength and (ii) multiple wavelengths. The first option (which retains the applicability of older version of JaSTA) calculates the light scattering properties of aggregates of spheres for a single wavelength at a given instant of time whereas the second option can execute the code for a multiple numbers of wavelengths in a single run. JaSTA-2 provides convenient and quicker data analysis which can be used in diverse fields like Planetary Science, Atmospheric Physics, Nanoscience, etc. This version of the software is developed for Linux platform only, and it can be operated over all the cores of a processor using the multi-threading option.

  16. Carbon farming in hot, dry coastal areas: an option for climate change mitigation

    NASA Astrophysics Data System (ADS)

    Becker, K.; Wulfmeyer, V.; Berger, T.; Gebel, J.; Münch, W.

    2013-07-01

    We present a comprehensive, interdisciplinary project which demonstrates that large-scale plantations of Jatropha curcas - if established in hot, dry coastal areas around the world - could capture 17-25 t of carbon dioxide per hectare per year from the atmosphere (over a 20 yr period). Based on recent farming results it is confirmed that the Jatropha curcas plant is well adapted to harsh environments and is capable of growing alone or in combination with other tree and shrub species with minimal irrigation in hot deserts where rain occurs only sporadically. Our investigations indicate that there is sufficient unused and marginal land for the widespread cultivation of Jatropha curcas to have a significant impact on atmospheric CO2 levels at least for several decades. In a system in which desalinated seawater is used for irrigation and for delivery of mineral nutrients, the sequestration costs were estimated to range from 42-63 EUR per tonne CO2. This result makes carbon farming a technology that is competitive with carbon capture and storage (CCS). In addition, high-resolution simulations using an advanced land-surface-atmosphere model indicate that a 10 000 km2 plantation could produce a reduction in mean surface temperature and an onset or increase in rain and dew fall at a regional level. In such areas, plant growth and CO2 storage could continue until permanent woodland or forest had been established. In other areas, salinization of the soil may limit plant growth to 2-3 decades whereupon irrigation could be ceased and the captured carbon stored as woody biomass.

  17. High-Rate Mechanical Properties of JA2 Propellant at Temperatures from -50 to 80 deg C

    DTIC Science & Technology

    2015-07-01

    panorama of postcompression JA2 grain sample (uniaxially compressed at a rate of ~100 s–1, 80 °C, and strain greater than 40%), 50× magnification...19 Fig. 36 SEM panorama of postcompression JA2 grain sample...19 Fig. 37 SEM panorama of postcompression JA2 grain sample (uniaxially compressed at a rate of ~100 s–1, 60 °C, and strain

  18. Gene expression and plant hormone levels in two contrasting rice genotypes responding to brown planthopper infestation.

    PubMed

    Li, Changyan; Luo, Chao; Zhou, Zaihui; Wang, Rui; Ling, Fei; Xiao, Langtao; Lin, Yongjun; Chen, Hao

    2017-02-28

    The brown planthopper (BPH; Nilaparvata lugens Stål) is a destructive piercing-sucking insect pest of rice. The plant hormones salicylic acid (SA) and jasmonic acid (JA) play important roles in plant-pest interactions. Many isolated rice genes that modulate BPH resistance are involved in the metabolism or signaling pathways of SA, JA and ethylene. 'Rathu Heenati' (RH) is a rice cultivar with a high-level, broad-spectrum resistance to all BPH biotypes. Here, RH was used as the research material, while a BPH-susceptible rice cultivar 'Taichung Native 1' (TN1) was the control. A cDNA microarray analysis illuminated the resistance response at the genome level of RH under BPH infestation. The levels of SA and JA in RH and TN1 seedlings after BPH infestation were also determined. The expression pattern clustering indicated that 1467 differential probe sets may be associated with constitutive resistance and 67 with the BPH infestation-responsive resistance of RH. A Venn diagram analysis revealed 192 RH-specific and BPH-inducible probe sets. Finally, 23 BPH resistance-related gene candidates were selected based on the expression pattern clustering and Venn diagram analysis. In RH, the SA content significantly increased and the JA content significantly decreased after BPH infestation, with the former occurring prior to the latter. In RH, the differential genes in the SA pathway were synthesis-related and were up-regulated after BPH infestation. The differential genes in the JA pathway were also up-regulated. They were jasmonate ZIM-domain transcription factors, which are important negative regulators of the JA pathway. Comparatively, genes involved in the ET pathway were less affected by a BPH infestation in RH. DNA sequence analysis revealed that most BPH infestation-inducible genes may be regulated by the genetic background in a trans-acting manner, instead of by their promoters. We profiled the analysis of the global gene expression in RH and TN1 under BPH infestation

  19. Constraints for nutritional grouping in Wisconsin and Michigan dairy farms.

    PubMed

    Contreras-Govea, F E; Cabrera, V E; Armentano, L E; Shaver, R D; Crump, P M; Beede, D K; VandeHaar, M J

    2015-02-01

    A survey was conducted in Wisconsin (WI) and Michigan (MI) to quantify the proportion of farms that use a single diet for all lactating cows and to better understand the reasons for current grouping strategies and the limitations to grouping for better nutritional management. A questionnaire was mailed to all WI dairy farmers with ≥200 lactating cows (971 farms) and to a random sample of grade-A MI dairy farmers (800 farms) of varying herd sizes. The survey return rate was 20% in WI (196 farms) and 26% in MI (211 farms; 59 of them had ≥200 lactating cows). Feeding 2 or more different diets to lactating cows was predominant: 63% in WI (124 farms, all ≥200 lactating cows), 76% in MI farms with ≥200 lactating cows (45 farms), and 28% in MI farms with <200 lactating cows (43 farms). Farmers feeding more than 1 diet used 1 or more of the following criteria for grouping lactating cows: stage of lactation, milk production, or body condition score. Overall for both states, 52% of the farms (211 from 407 farms) feeding more than 1 diet grouped cows according to their nutritional needs. However, a notable population of farms fed the same diet to all lactating cows: 37% in WI (72 farms), 24% in MI (14 farms) for herds of ≥200 lactating cows, and 72% in MI for herds of <200 lactating cows (109 MI farms). "Desire to keep it simple" and "milk drops when cows are moved to a different group" were identified as main constraints to having more groups within a farm for nutritional purposes. Farm facilities and labor were also limiting factors to grouping in farms with herd sizes of <200 lactating cows. Copyright © 2015 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

  20. [Transgenic plants as medicine production systems].

    PubMed

    Okada, Y

    1997-10-01

    Transgenic plants are emerging as an important system for the expression of many recombinant proteins, especially those intended for therapeutic purpose. The production of foreign proteins in plants has several advantages. In terms of required equipment and cost, mass production in plants is far easier to achieve than techniques involving animal cells. Successful production of several proteins in plants, including human serum albumin, haemoglobin, monoclonal antibodies, viral antigens (vaccines), enkephalin, and trichosanthin, has been reported. Particularly, the demonstration that vaccine antigens can be produced in plants in their native, immunogenic forms opens exciting possibilities for the "bio-farming" of vaccines. If the antigens are orally active, food-based "edible vaccines" could allow economical production. In this review, I will discuss the progress that has been made by several groups in what is now an expanding area of medicine research that utilizes transgenic plants.

  1. Plant growth regulator-mediated anti-herbivore responses of cabbage (Brassica oleracea) against cabbage looper Trichoplusia ni Hübner (Lepidoptera: Noctuidae).

    PubMed

    Scott, Ian M; Samara, R; Renaud, J B; Sumarah, M W

    2017-09-01

    Plant elicitors can be biological or chemical-derived stimulators of jasmonic acid (JA) or salicylic acid (SA) pathways shown to prime the defenses in many crops. Examples of chemical elicitors of the JA and SA pathways include methyl-jasmonate and 1,2,3-benzothiadiazole-7-carbothioate (BTH or the commercial plant activator Actigard 50WG, respectively). The use of specific elicitors has been observed to affect the normal interaction between JA and SA pathways causing one to be upregulated and the other to be suppressed, often, but not always, at the expense of the plant's herbivore or pathogen defenses. The objective of this study was to determine whether insects feeding on Brassica crops might be negatively affected by SA inducible defenses combined with an inhibitor of detoxification and anti-oxidant enzymes that regulate the insect response to the plant's defenses. The relative growth rate of cabbage looper Trichoplusia ni Hübner (Lepidoptera: Noctuidae) fed induced cabbage Brassica oleraceae leaves with the inhibitor, quercetin, was significantly less than those fed control cabbage with and without the inhibitor. The reduced growth was related to the reduction of glutathione S-transferases (GSTs) by the combination of quercetin and increased levels of indole glucosinolates in the cabbage treated with BTH at 2.6× the recommended application rate. These findings may offer a novel combination of elicitor and synergist that can provide protection from plant disease and herbivores in cabbage and other Brassica crops. Crown Copyright © 2016. Published by Elsevier Inc. All rights reserved.

  2. Notes on Methods for Studying Farm Women.

    ERIC Educational Resources Information Center

    Colman, Gould P.

    1981-01-01

    Supports several of Frances Hill's positions on research about farm women (RC 504 387). States that regarding farm and family as separate dynamic systems whose integration varies in differing situations provides a context for evaluating impacts on farm and family. Available from: Rural Sociological Society, 325 Morgan Hall, University of…

  3. American Farm Bureau Foundation for Agriculture - Homepage

    Science.gov Websites

    Literacy? What We Do Resources Sustainable Agriculture Food and Farm Facts Free Resources & Lesson Agriculture Food and Farm Facts Free Resources & Lesson Plans Bringing Biotech to Life Learn About Beef Farm For Teachers Free Resources Outreach Team Grow your Ag Literacy Impact Career Spotlight

  4. Incorporation and the Farm Family.

    ERIC Educational Resources Information Center

    Salamon, Sonya; Markan, Kathleen K.

    1984-01-01

    Studied Illinois farm households (N=13), some already incorporated from five to ten years, regarding ethnic values and intergenerational land transfers. Concluded that farm families need to question whether the tax savings involved with incorporation are worth the potential complications such as estate settlement, marital satisfaction, migration,…

  5. Farm Business Management Analysis: Adjusting the Farm Business to Increase Profit. Unit III. Volume 15, Number 3. Instructor's Guide.

    ERIC Educational Resources Information Center

    Denker, Robert; And Others

    Designed primarily for Missouri vocational agricultural instructors participating in the Farm Business Management Analysis Program, this instructor's guide, consisting of 10 lessons, deals with adjusting a farm business to increase profits. The following topics are covered in the individual lessons: law and the farm family, planning income tax…

  6. Methods and systems for seed planting management and control

    DOEpatents

    Svoboda, John M.; Hess, J. Richard; Hoskinson, Reed L.; Harker, David J.

    2002-01-01

    A seed planting system providing optimal seed spacing in an agricultural field. The seed planting system includes a mobile seed planter having one or more planting shoes, or members being adapted for towing by a farm vehicle or being self-propelled. Sensors, disposed proximate to respective planting shoes, detect seed planting events and send corresponding signals to a computer. Contemporaneously, a geospatial locator acquires, and transmits to the computer, the geospatial location of each planted seed. The computer correlates the geospatial location data with the seed deposition data and generates a seed distribution profile indicating the location of each seed planted in a zone of interest to enable the control of speed spacing.

  7. Effects of stored feed cropping systems and farm size on the profitability of Maine organic dairy farm simulations.

    PubMed

    Hoshide, A K; Halloran, J M; Kersbergen, R J; Griffin, T S; DeFauw, S L; LaGasse, B J; Jain, S

    2011-11-01

    United States organic dairy production has increased to meet the growing demand for organic milk. Despite higher prices received for milk, organic dairy farmers have come under increasing financial stress due to increases in concentrated feed prices over the past few years, which can make up one-third of variable costs. Market demand for milk has also leveled in the last year, resulting in some downward pressure on prices paid to dairy farmers. Organic dairy farmers in the Northeast United States have experimented with growing different forage and grain crops to maximize on-farm production of protein and energy to improve profitability. Three representative organic feed systems were simulated using the integrated farm system model for farms with 30, 120, and 220 milk cows. Increasing intensity of equipment use was represented by organic dairy farms growing only perennial sod (low) to those with corn-based forage systems, which purchase supplemental grain (medium) or which produce and feed soybeans (high). The relative profitability of these 3 organic feed systems was strongly dependent on dairy farm size. From results, we suggest smaller organic dairy farms can be more profitable with perennial sod-based rather than corn-based forage systems due to lower fixed costs from using only equipment associated with perennial forage harvest and storage. The largest farm size was more profitable using a corn-based system due to greater economies of scale for growing soybeans, corn grain, winter cereals, and corn silages. At an intermediate farm size of 120 cows, corn-based forage systems were more profitable if perennial sod was not harvested at optimum quality, corn was grown on better soils, or if milk yield was 10% higher. Delayed harvest decreased the protein and energy content of perennial sod crops, requiring more purchased grain to balance the ration and resulting in lower profits. Corn-based systems were less affected by lower perennial forage quality, as corn silage

  8. Farmed Atlantic salmon: potential invader in the Pacific Northwest?

    Treesearch

    Jonathan Thompson; Pete Bisson

    2008-01-01

    Commercial farming of Atlantic salmon in marine net-pens has become a booming industry. At present, approximately 130 salmon farms exist along the Pacific coast of North America. Most of these farms are in cold marine bays within British Columbia, where farmed salmon have become the province’s most valuable agricultural export. Each year, thousands of farmed Atlantic...

  9. Fun on the farm: evaluation of a lesson to teach students about the spread of infection on school farm visits.

    PubMed

    Hawking, Meredith K D; Lecky, Donna M; Verlander, Neville Q; McNulty, Cliodna A M

    2013-01-01

    School visits to farms are a positive educational experience but pose risks due to the spread of zoonotic infections. A lesson plan to raise awareness about microbes on the farm and preventative behaviours was developed in response to the Griffin Investigation into the E. coli outbreak associated with Godstone Farm in 2009. This study evaluated the effectiveness of the delivery of the lesson plan in increasing knowledge about the spread of infection on the farm, amongst school students. Two hundred and twenty-five 9-11 year old students from seven junior schools in England participated. Two hundred and ten students filled in identical questionnaires covering microbes, hand hygiene, and farm hygiene before and after the lesson. Statistical analysis assessed knowledge change using difference in percentage correct answers. Significant knowledge improvement was observed for all sections. In the 'Farm Hygiene' section, girls and boys demonstrated 18% (p<0.001) and 11% (p<0.001) improvement, respectively (girls vs. boys p<0.004). As girls had lower baseline knowledge the greater percentage improvement resulted in similar post intervention knowledge scores between genders (girls 80%, boys 83%). The lesson plan was successful at increasing awareness of microbes on the farm and infection prevention measures and should be used by teachers in preparation for a farm visit.

  10. Iowa Farm and Rural Life Poll. Summary.

    ERIC Educational Resources Information Center

    Lasley, Paul

    The 1984 Iowa Farm and Rural Life Poll is summarized in this report. Responses from 1,585 randomly selected Iowa farm families showed that respondents opposed relaxing current state laws limiting foreign investors and non-farm corporations' ownership of farmland; had mixed feelings on absentee ownership, changing banking laws to allow banks to…

  11. Farm Animals

    MedlinePlus

    ... accidentally breathe in or swallow spores in contaminated soil, food, or water. The greatest risk is from ... spread to people and animals through contaminated food, soil, or water. Farm animals that are at risk ...

  12. Drying of medicinal plants with solar energy utilization

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Wisniewski, G.

    In the paper, a potential of solar energy for drying of medicinal plants in Polish conditions is estimated and development of solar drying technologies is presented. The results of economic assessment of flat-plate solar collectors applied for drying of medicinal plants on a farm are promising. In some specific conditions, e.g. drying of wild grown medicinal plants in remote areas, even application of photovoltaic modules for driving of a fan of a solar dryer is a profitable option and enables easy control of the drying air temperature.

  13. Empirical Analysis of Farm Credit Risk under the Structure Model

    ERIC Educational Resources Information Center

    Yan, Yan

    2009-01-01

    The study measures farm credit risk by using farm records collected by Farm Business Farm Management (FBFM) during the period 1995-2004. The study addresses the following questions: (1) whether farm's financial position is fully described by the structure model, (2) what are the determinants of farm capital structure under the structure model, (3)…

  14. The effects of plant growth regulators and L-phenylalanine on phenolic compounds of sweet basil.

    PubMed

    Koca, Nülüfer; Karaman, Şengül

    2015-01-01

    The effects of methyl jasmonate (MeJA), spermine (Spm), epibrassinolide (EBL) and l-phenylalanine on sweet basil (Ocimum basilicum L.) were studied to determine the amount of phenolic compounds and enzymatic activity of phenylalanine ammonia-lyase (PAL). Total phenolic and total flavonoid contents of sweet basils were determined by a spectrophotometer, and individual phenolic compounds and activity of PAL were analysed by HPLC/UV. The highest total phenolic (6.72 mg GAE/g) and total flavonoid contents (0.92 mg QE/g) obtained from 1.0 mM Spm+MeJA application. Rosmarinic acid (RA) and caffeic acid contents significantly enhanced after the applications but no such differences observed in chicoric acid content or PAL activity. RA was the main phenolic acid in all samples and its concentration varied from 1.04 to 2.70 mg/gFW. As a result the combinations of Spm+MeJA and EBL+MeJA can induce secondary metabolites effectively and those interactions play important role in the production of phytochemicals in plants. Copyright © 2014 Elsevier Ltd. All rights reserved.

  15. Attitudes of farm consultants in the South Island of New Zealand towards the role of veterinarians in farm businesses.

    PubMed

    Bates, A J; Wapenaar, W; Campbell, A C; Eggleton, R G

    2016-09-01

    To assess the attitude of dairy farm consultants to the role of veterinarians on dairy farms and whether they viewed veterinary advice as impartial, free from commercial bias and central to farm productivity and profitability. A telephone survey was commissioned in September 2014 of 36 full-time farm consultants in the Canterbury and Otago region of New Zealand using a pre-prepared questionnaire to explore their attitudes towards the veterinary profession. Of those approached, 25 completed the survey. The consultants surveyed agreed or strongly agreed (21/23) that they were the custodians of animal health expenditure on farms, which was seen as an expense to be controlled (19/25 agreed or strongly agreed). This view was held more strongly (9/9 agreed or strongly agreed) by consultants with >5 years' professional experience, compared to 10/16 consultants with ≤5 years' experience. Most consultants (24/25) disagreed that they did not respect veterinary advice, but agreed (24/25) that veterinarians almost always had a vested interest in the advice that they gave. The role of veterinarians was seen by respondents to be mainly treatment of sick animals (22/25) and provision of animal health products (24/25), but not in maximising farm profitability (selected by 8/25 respondents). Consultants viewed their own roles as providing advice on the influence of animal health on farm profitability (21/25), nutrition (22/25) and reproduction (20/25). Most respondents (21/25) stated that reducing disease and animal health issues was important, but they (21/25) also identified a reduction in farm animal health expenditure as a measure of success. Only 7/25 consultants felt that they should work in partnership with veterinarians. This was a small scale study from one region of New Zealand but it indicates that consultants are ambivalent about the role and value of working more closely with veterinarians, and about the benefit that veterinary intervention may bring to a farm

  16. Modeling greenhouse gas emissions from dairy farms.

    PubMed

    Rotz, C Alan

    2017-11-15

    Dairy farms have been identified as an important source of greenhouse gas emissions. Within the farm, important emissions include enteric CH 4 from the animals, CH 4 and N 2 O from manure in housing facilities during long-term storage and during field application, and N 2 O from nitrification and denitrification processes in the soil used to produce feed crops and pasture. Models using a wide range in level of detail have been developed to represent or predict these emissions. They include constant emission factors, variable process-related emission factors, empirical or statistical models, mechanistic process simulations, and life cycle assessment. To fully represent farm emissions, models representing the various emission sources must be integrated to capture the combined effects and interactions of all important components. Farm models have been developed using relationships across the full scale of detail, from constant emission factors to detailed mechanistic simulations. Simpler models, based upon emission factors and empirical relationships, tend to provide better tools for decision support, whereas more complex farm simulations provide better tools for research and education. To look beyond the farm boundaries, life cycle assessment provides an environmental accounting tool for quantifying and evaluating emissions over the full cycle, from producing the resources used on the farm through processing, distribution, consumption, and waste handling of the milk and dairy products produced. Models are useful for improving our understanding of farm processes and their interacting effects on greenhouse gas emissions. Through better understanding, they assist in the development and evaluation of mitigation strategies for reducing emissions and improving overall sustainability of dairy farms. The Authors. Published by the Federation of Animal Science Societies and Elsevier Inc. on behalf of the American Dairy Science Association®. This is an open access article

  17. Risk-based audit selection of dairy farms.

    PubMed

    van Asseldonk, M A P M; Velthuis, A G J

    2014-02-01

    Dairy farms are audited in the Netherlands on numerous process standards. Each farm is audited once every 2 years. Increasing demands for cost-effectiveness in farm audits can be met by introducing risk-based principles. This implies targeting subpopulations with a higher risk of poor process standards. To select farms for an audit that present higher risks, a statistical analysis was conducted to test the relationship between the outcome of farm audits and bulk milk laboratory results before the audit. The analysis comprised 28,358 farm audits and all conducted laboratory tests of bulk milk samples 12 mo before the audit. The overall outcome of each farm audit was classified as approved or rejected. Laboratory results included somatic cell count (SCC), total bacterial count (TBC), antimicrobial drug residues (ADR), level of butyric acid spores (BAB), freezing point depression (FPD), level of free fatty acids (FFA), and cleanliness of the milk (CLN). The bulk milk laboratory results were significantly related to audit outcomes. Rejected audits are likely to occur on dairy farms with higher mean levels of SCC, TBC, ADR, and BAB. Moreover, in a multivariable model, maxima for TBC, SCC, and FPD as well as standard deviations for TBC and FPD are risk factors for negative audit outcomes. The efficiency curve of a risk-based selection approach, on the basis of the derived regression results, dominated the current random selection approach. To capture 25, 50, or 75% of the population with poor process standards (i.e., audit outcome of rejected), respectively, only 8, 20, or 47% of the population had to be sampled based on a risk-based selection approach. Milk quality information can thus be used to preselect high-risk farms to be audited more frequently. Copyright © 2014 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

  18. Hormone crosstalk in plant disease and defense: more than just jasmonate-salicylate antagonism.

    PubMed

    Robert-Seilaniantz, Alexandre; Grant, Murray; Jones, Jonathan D G

    2011-01-01

    Until recently, most studies on the role of hormones in plant-pathogen interactions focused on salicylic acid (SA), jasmonic acid (JA), and ethylene (ET). It is now clear that pathogen-induced modulation of signaling via other hormones contributes to virulence. A picture is emerging of complex crosstalk and induced hormonal changes that modulate disease and resistance, with outcomes dependent on pathogen lifestyles and the genetic constitution of the host. Recent progress has revealed intriguing similarities between hormone signaling mechanisms, with gene induction responses often achieved by derepression. Here, we report on recent advances, updating current knowledge on classical defense hormones SA, JA, and ET, and the roles of auxin, abscisic acid (ABA), cytokinins (CKs), and brassinosteroids in molding plant-pathogen interactions. We highlight an emerging theme that positive and negative regulators of these disparate hormone signaling pathways are crucial regulatory targets of hormonal crosstalk in disease and defense. Copyright © 2011 by Annual Reviews. All rights reserved.

  19. Gaseous 3-pentanol primes plant immunity against a bacterial speck pathogen, Pseudomonas syringae pv. tomato via salicylic acid and jasmonic acid-dependent signaling pathways in Arabidopsis.

    PubMed

    Song, Geun C; Choi, Hye K; Ryu, Choong-Min

    2015-01-01

    3-Pentanol is an active organic compound produced by plants and is a component of emitted insect sex pheromones. A previous study reported that drench application of 3-pentanol elicited plant immunity against microbial pathogens and an insect pest in crop plants. Here, we evaluated whether 3-pentanol and the derivatives 1-pentanol and 2-pentanol induced plant systemic resistance using the in vitro I-plate system. Exposure of Arabidopsis seedlings to 10 μM and 100 nM 3-pentanol evaporate elicited an immune response to Pseudomonas syringae pv. tomato DC3000. We performed quantitative real-time PCR to investigate the 3-pentanol-mediated Arabidopsis immune responses by determining Pathogenesis-Related (PR) gene expression levels associated with defense signaling through salicylic acid (SA), jasmonic acid (JA), and ethylene signaling pathways. The results show that exposure to 3-pentanol and subsequent pathogen challenge upregulated PDF1.2 and PR1 expression. Selected Arabidopsis mutants confirmed that the 3-pentanol-mediated immune response involved SA and JA signaling pathways and the NPR1 gene. Taken together, this study indicates that gaseous 3-pentanol triggers induced resistance in Arabidopsis by priming SA and JA signaling pathways. To our knowledge, this is the first report that a volatile compound of an insect sex pheromone triggers plant systemic resistance against a bacterial pathogen.

  20. Green Care: A Review of the Benefits and Potential of Animal-Assisted Care Farming Globally and in Rural America

    PubMed Central

    Artz, Brianna; Bitler Davis, Doris

    2017-01-01

    Simple Summary The term Green Care encompasses a number of therapeutic strategies that can include farm-animal-assisted therapy, horticultural therapy, and general, farm-based therapy. This review article provides an overview of how Green Care has been used as part of the therapeutic plan for a variety of psychological disorders and related physical disabilities in children, adolescents and adults. While many countries have embraced Green Care, and research-based evidence supports its efficacy in a variety of therapeutic models, it has not yet gained widespread popularity in the United States. We suggest that Green Care could prove to be an effective approach to providing mental health care in the U.S., particularly in rural areas that are typically underserved by more traditional mental health facilities, but have an abundance of farms, livestock, and green spaces where care might be effectively provided. Abstract The term Green Care includes therapeutic, social or educational interventions involving farming; farm animals; gardening or general contact with nature. Although Green Care can occur in any setting in which there is interaction with plants or animals, this review focuses on therapeutic practices occurring on farms. The efficacy of care farming is discussed and the broad utilization of care farming and farm care communities in Europe is reviewed. Though evidence from care farms in the United States is included in this review, the empirical evidence which could determine its efficacy is lacking. For example, the empirical evidence supporting or refuting the efficacy of therapeutic horseback riding in adults is minimal, while there is little non-equine care farming literature with children. The health care systems in Europe are also much different than those in the United States. In order for insurance companies to cover Green Care techniques in the United States, extensive research is necessary. This paper proposes community-based ways that Green Care

  1. Precipitation-runoff relations and water-quality characteristics at edge-of-field stations, Discovery Farms and Pioneer Farm, Wisconsin, 2003-8

    USGS Publications Warehouse

    Stuntebeck, Todd D.; Komiskey, Matthew J.; Peppler, Marie C.; Owens, David W.; Frame, Dennis R.

    2011-01-01

    A cooperative study between the U.S. Geological Survey, the University of Wisconsin (UW)-Madison Discovery Farms program (Discovery Farms), and the UW-Platteville Pioneer Farm program (Pioneer Farm) was developed to identify typical ranges and magnitudes, temporal distributions, and principal factors affecting concentrations and yields of sediment, nutrients, and other selected constituents in runoff from agricultural fields. Hydrologic and water-quality data were collected year-round at 23 edge-of-field monitoring stations on 5 privately owned Discovery Farms and on Pioneer Farm during water years 2003-8. The studied farms represented landscapes, soils, and farming systems typical of livestock farms throughout southern Wisconsin. Each farm employed a variety of soil, nutrient, and water-conservation practices to help minimize sediment and nutrient losses from fields and to improve crop productivity. This report summarizes the precipitation-runoff relations and water-quality characteristics measured in edge-of-field runoff for 26 "farm years" (aggregate years of averaged station data from all 6 farms for varying monitoring periods). A relatively wide range of constituents typically found in agricultural runoff were measured: suspended sediment, phosphorus (total, particulate, dissolved reactive, and total dissolved), and nitrogen (total, nitrate plus nitrite, organic, ammonium, total Kjeldahl and total Kjeldahl-dissolved), chloride, total solids, total suspended solids, total volatile suspended solids, and total dissolved solids. Mean annual precipitation was 32.8 inches for the study period, about 3 percent less than the 30-year mean. Overall mean annual runoff was 2.55 inches per year (about 8 percent of precipitation) and the distribution was nearly equal between periods of frozen ground (54 percent) and unfrozen ground (46 percent). Mean monthly runoff was highest during two periods: February to March and May to June. Ninety percent of annual runoff occurred

  2. Social environments, risk-taking and injury in farm adolescents

    PubMed Central

    Pickett, William; Berg, Richard L; Marlenga, Barbara

    2017-01-01

    Background Farm environments are especially hazardous for young people. While much is known about acute physical causes of traumatic farm injury, little is known about social factors that may underlie their aetiology. Objectives In a nationally representative sample of young Canadians aged 11–15 years, we described and compared farm and non-farm adolescents in terms of the qualities of their social environments, engagement in overt multiple risk-taking as well as how such exposures relate aetiologically to their reported injury experiences. Methods Cross-sectional analysis of survey reports from the 2014 (Cycle 7) Canadian Health Behaviour in School-Aged Children study was conducted. Children (n=2567; 2534 weighted) who reported living or working on farms were matched within schools in a 1:1 ratio with children not living or working on farms. Scales examining quality of social environments and overt risk-taking were compared between the two groups, stratified by gender. We then related the occurrence of any serious injury to these social exposures in direct and interactive models. Results Farm and non-farm children reported social environments that were quite similar, with the exception of overt multiple risk-taking, which was demonstrably higher in farm children of both genders. Engagement in overt risk-taking, but not the other social environmental factors, was strongly and consistently associated with risks for serious injury in farm as well as non-farm children, particularly among males. Conclusions Study findings highlight the strength of associations between overt multiple risk-taking and injury among farm children. This appears to be a normative aspect of adolescent farm culture. PMID:28137978

  3. Exploring contacts facilitating transmission of influenza A(H5N1) virus between poultry farms in West Java, Indonesia: A major role for backyard farms?

    PubMed

    Wibawa, Hendra; Karo-Karo, Desniwaty; Pribadi, Eko Sugeng; Bouma, Annemarie; Bodewes, Rogier; Vernooij, Hans; Diyantoro; Sugama, Agus; Muljono, David H; Koch, Guus; Tjatur Rasa, Fadjar Sumping; Stegeman, Arjan

    2018-08-01

    Highly pathogenic avian influenza virus (HPAIV) H5N1 has been reported in Asia, including Indonesia since 2003. Although several risk factors related to the HPAIV outbreaks in poultry in Indonesia have been identified, little is known of the contact structure of farms of different poultry production types (backyard chickens, broilers, layers, and ducks). This study aims to quantify the contact rates associated with the movement of people, and movements of live birds and products and equipment that affect the risk of HPAIV H5N1 transmission between poultry farms in Indonesia. On 124 poultry farms in 6 districts in West Java, logbooks were distributed to record the movements of farmers/staff and visitors and their poultry contacts. Most movements in backyard chicken, commercial native chicken, broiler and duck farms were visits to and from other poultry farms, whilst in layer farms visits to and from poultry companies, visits to egg collection houses and visit from other poultry farms were most frequent. Over 75% of persons visiting backyard chicken and duck farms had previously visited other poultry farms on the same day. Visitors of backyard chicken farms had the highest average contact rate, either direct contact with poultry on other farms before the visits (1.35 contact/day) or contact during their visits in the farms (10.03 contact/day). These results suggest that backyard chicken farms are most at risk for transmission of HPAIV compared to farms of the other poultry production types. Since visits of farm-to-farm were high, backyard farms could also a potential source for HPAIV transmission to commercial poultry farms. Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.

  4. The U.S. Farm Problem.

    ERIC Educational Resources Information Center

    Alston, Lee

    1985-01-01

    Intended to provide teachers with an analysis of contemporary United States agriculture, this analysis begins with an overview of the farm community focusing on the income distinction between wealthy commercial farmers and other farmers. The cause of farm unrest is that earnings are too low relative to expenses. The problems of declining farm…

  5. Grieving for the Family Farm.

    ERIC Educational Resources Information Center

    Zeller, Simon H.

    1986-01-01

    Reviews impact of recent agricultural trends in South Dakota. Outlines Kubler-Ross' stages of grief/adaptation that farm families must negotiate as they cope with the trauma of the loss of their farms. Indicates service providers must overcome farmers' mistrust for human welfare services and reach out to this vulnerable population. (NEC)

  6. Using a whole farm model to determine the impacts of mating management on the profitability of pasture-based dairy farms.

    PubMed

    Beukes, P C; Burke, C R; Levy, G; Tiddy, R M

    2010-08-01

    An approach to assessing likely impacts of altering reproductive performance on productivity and profitability in pasture-based dairy farms is described. The basis is the development of a whole farm model (WFM) that simulates the entire farm system and holistically links multiple physical performance factors to profitability. The WFM consists of a framework that links a mechanistic cow model, a pasture model, a crop model, management policies and climate. It simulates individual cows and paddocks, and runs on a day time-step. The WFM was upgraded to include reproductive modeling capability using reference tables and empirical equations describing published relationships between cow factors, physiology and mating management. It predicts reproductive status at any time point for individual cows within a modeled herd. The performance of six commercial pasture-based dairy farms was simulated for the period of 12 months beginning 1 June 2005 (05/06 year) to evaluate the accuracy of the model by comparison with actual outcomes. The model predicted most key performance indicators within an acceptable range of error (residual<10% of observed). The evaluated WFM was then used for the six farms to estimate the profitability of changes in farm "set-up" (farm conditions at the start of the farming year on 1 June) and mating management from 05/06 to 06/07 year. Among the six farms simulated, the 4-week calving rate emerged as an important set-up factor influencing profitability, while reproductive performance during natural bull mating was identified as an area with the greatest opportunity for improvement. The WFM presents utility to explore alternative management strategies to predict likely outcomes to proposed changes to a pasture-based farm system. Copyright (c) 2010 Elsevier B.V. All rights reserved.

  7. The effects of farm management practices on liver fluke prevalence and the current internal parasite control measures employed on Irish dairy farms.

    PubMed

    Selemetas, Nikolaos; Phelan, Paul; O'Kiely, Padraig; de Waal, Theo

    2015-01-30

    Fasciolosis caused by Fasciola hepatica is responsible for major production losses in cattle farms. The objectives of this study were to assess the effect of farm management practices on liver fluke prevalence on Irish dairy farms and to document the current control measures against parasitic diseases. In total, 369 dairy farms throughout Ireland were sampled from October to December 2013, each providing a single bulk tank milk (BTM) sample for liver fluke antibody-detection ELISA testing and completing a questionnaire on their farm management. The analysis of samples showed that cows on 78% (n=288) of dairy farms had been exposed to liver fluke. There was a difference (P<0.05) between farms where cows were positive or negative for liver fluke antibodies in (a) the total number of adult dairy cows in herds, (b) the number of adult dairy cows contributing to BTM samples, and (c) the size of the total area of grassland, with positive farms having larger numbers in each case. There was no difference (P>0.05) between positive and negative farms in (a) the grazing of dry cows together with replacement cows, (b) whether or not grazed grassland was mowed for conservation, (c) the type of drinking water provision system, (d) spreading of cattle manure on grassland or (e) for grazing season length (GSL; mean=262.5 days). Also, there were differences (P<0.001) between drainage statuses for GSL with farms on good drainage having longer GSL than moderately drained farms. The GSL for dairy cows on farms with good drainage was 11 days longer than for those with moderate drainage (P<0.001). The percentage of farmers that used an active ingredient during the non-lactating period against liver fluke, gastrointestinal nematodes, lungworm, and rumen fluke was 96%, 85%, 77% and 90%, respectively. Albendazole was the most frequently used active ingredient for treatment against gastrointestinal nematodes (57%), liver fluke (40%) and lungworm (47%), respectively. There was a difference

  8. 78 FR 63158 - Information Collection; Guaranteed Farm Loan Program

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-23

    ... DEPARTMENT OF AGRICULTURE Farm Service Agency Information Collection; Guaranteed Farm Loan Program... collection associated with the Guaranteed Farm Loan Program. The amended estimate adds the merger of the information collection for the Land Contract Guarantee Program (0560-0279) into the Guaranteed Farm Loan...

  9. 78 FR 63380 - Farm Credit Administration Board Policy Statements

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-24

    ... FARM CREDIT ADMINISTRATION 12 CFR Chapter VI Farm Credit Administration Board Policy Statements AGENCY: Farm Credit Administration. ACTION: Notice of policy statements and index. SUMMARY: The Farm Credit Administration (FCA), as part of its annual public notification process, is publishing for notice...

  10. Will He Take Over? A Longitudinal, Five County Study of Patterns of Establishment in Farming by Wisconsin's Farm Youth.

    ERIC Educational Resources Information Center

    Bjoraker, W. T.; And Others

    The main purpose of this investigation was to determine patterns of establishment in farming followed by youth in five Wisconsin counties. Specific objectives were: (1) to determine who among the 1957 high-school graduates had ever farmed and were actually farming in 1968; (2) to determine the similarities and differences of those who stayed in…

  11. Within-farm variability in age structure of breeding-female pigs and reproductive performance on commercial swine breeding farms.

    PubMed

    Koketsu, Yuzo

    2005-03-15

    This study investigated relationships between herd age structure and herd productivity in breeding herds; it also investigated a pattern in parity proportions of females over 2 years and its relationship with herd productivity in commercial swine herds. This study was based on data from 148 commercial farms in North America stored in the swine database program at the University of Minnesota. The primary selection criterion was fluctuations in breeding-female pig (female) inventories over a 2-year interval. Productivity measurements and parity proportions of females were extracted from the database. A 24-month time-plot in proportions of Parity 0 and Parities 3-5 females (mid-parity) was charted for each farm. Using these charts, a change in proportions of Parity 0 and mid-parity for each farm was categorized into patterns: FLUCTUATE (Parity 0 and mid-parity proportion lines crossed) or STABLE (the two proportion lines never crossed). Higher proportions of mid-parity sows were correlated with greater pigs weaned per female per year (PWFY; P < 0.01). Farms with a FLUCTUATE (73% of the 148 farms) pattern had lower PWFY than those with a STABLE pattern (P < 0.01). The STABLE farms had higher proportions of mid-parity sows, higher parity at culling, higher frequency of gilt deliveries per year, and lower replacement rate than the FLUCTUATE farms (P < 0.01). In conclusion, maintaining stable subpopulations with mid-parity and Parity 0 are recommended to optimize herd productivity.

  12. Reduced soil cultivation and organic fertilization on organic farms: effects on crop yield and soil physical traits

    NASA Astrophysics Data System (ADS)

    Surböck, Andreas; Gollner, Gabriele; Klik, Andreas; Freyer, Bernhard; Friedel, Jürgen K.

    2017-04-01

    A continuous investment in soil fertility is necessary to achieve sustainable yields in organic arable farming. Crucial factors here besides the crop rotation are organic fertilization and the soil tillage system. On this topic, an operational group (Project BIOBO*) was established in the frame of an European Innovation Partnership in 2016 consisting of organic farmers, consultants and scientists in the farming region of eastern Austria. The aim of this group is the development and testing of innovative, reduced soil cultivation, green manure and organic fertilization systems under on-farm and on-station conditions to facilitate the sharing and transfer of experience and knowledge within and outside the group. Possibilities for optimization of the farm-specific reduced soil tillage system in combination with green manuring are being studied in field trials on six organic farms. The aim is to determine, how these measures contribute to an increase in soil organic matter contents, yields and income, to an improved nitrogen and nutrient supply to the crops, as well as support soil fertility in general. Within a long-term monitoring project (MUBIL), the effects of different organic fertilization systems on plant and soil traits have been investigated since 2003, when the farm was converted to organic management. The examined organic fertilization systems, i.e. four treatments representing stockless and livestock keeping systems, differ in lucerne management and the supply of organic manure (communal compost, farmyard manure, digestate from a biogas plant). Previous results of this on-station experiment have shown an improvement of some soil properties, especially soil physical properties, since 2003 in all fertilization systems and without differences between them. The infiltration rate of rainwater has increased because of higher hydraulic conductivity. The aggregate stability has shown also positive trends, which reduces the susceptibility to soil erosion by wind and

  13. The Resistome of Farmed Fish Feces Contributes to the Enrichment of Antibiotic Resistance Genes in Sediments below Baltic Sea Fish Farms

    PubMed Central

    Muziasari, Windi I.; Pitkänen, Leena K.; Sørum, Henning; Stedtfeld, Robert D.; Tiedje, James M.; Virta, Marko

    2017-01-01

    Our previous studies showed that particular antibiotic resistance genes (ARGs) were enriched locally in sediments below fish farms in the Northern Baltic Sea, Finland, even when the selection pressure from antibiotics was negligible. We assumed that a constant influx of farmed fish feces could be the plausible source of the ARGs enriched in the farm sediments. In the present study, we analyzed the composition of the antibiotic resistome from the intestinal contents of 20 fish from the Baltic Sea farms. We used a high-throughput method, WaferGen qPCR array with 364 primer sets to detect and quantify ARGs, mobile genetic elements (MGE), and the 16S rRNA gene. Despite a considerably wide selection of qPCR primer sets, only 28 genes were detected in the intestinal contents. The detected genes were ARGs encoding resistance to sulfonamide (sul1), trimethoprim (dfrA1), tetracycline [tet(32), tetM, tetO, tetW], aminoglycoside (aadA1, aadA2), chloramphenicol (catA1), and efflux-pumps resistance genes (emrB, matA, mefA, msrA). The detected genes also included class 1 integron-associated genes (intI1, qacEΔ1) and transposases (tnpA). Importantly, most of the detected genes were the same genes enriched in the farm sediments. This preliminary study suggests that feces from farmed fish contribute to the ARG enrichment in farm sediments despite the lack of contemporaneous antibiotic treatments at the farms. We observed that the intestinal contents of individual farmed fish had their own resistome compositions. Our result also showed that the total relative abundances of transposases and tet genes were significantly correlated (p = 0.001, R2 = 0.71). In addition, we analyzed the mucosal skin and gill filament resistomes of the farmed fish but only one multidrug-efflux resistance gene (emrB) was detected. To our knowledge, this is the first study reporting the resistome of farmed fish using a culture-independent method. Determining the possible sources of ARGs, especially

  14. The Resistome of Farmed Fish Feces Contributes to the Enrichment of Antibiotic Resistance Genes in Sediments below Baltic Sea Fish Farms.

    PubMed

    Muziasari, Windi I; Pitkänen, Leena K; Sørum, Henning; Stedtfeld, Robert D; Tiedje, James M; Virta, Marko

    2016-01-01

    Our previous studies showed that particular antibiotic resistance genes (ARGs) were enriched locally in sediments below fish farms in the Northern Baltic Sea, Finland, even when the selection pressure from antibiotics was negligible. We assumed that a constant influx of farmed fish feces could be the plausible source of the ARGs enriched in the farm sediments. In the present study, we analyzed the composition of the antibiotic resistome from the intestinal contents of 20 fish from the Baltic Sea farms. We used a high-throughput method, WaferGen qPCR array with 364 primer sets to detect and quantify ARGs, mobile genetic elements (MGE), and the 16S rRNA gene. Despite a considerably wide selection of qPCR primer sets, only 28 genes were detected in the intestinal contents. The detected genes were ARGs encoding resistance to sulfonamide ( sul1 ), trimethoprim ( dfrA1 ), tetracycline [ tet(32), tetM, tetO, tetW ], aminoglycoside ( aadA1, aadA2 ), chloramphenicol ( catA1 ), and efflux-pumps resistance genes ( emrB, matA, mefA, msrA ). The detected genes also included class 1 integron-associated genes ( intI1, qacE Δ 1 ) and transposases ( tnpA ). Importantly, most of the detected genes were the same genes enriched in the farm sediments. This preliminary study suggests that feces from farmed fish contribute to the ARG enrichment in farm sediments despite the lack of contemporaneous antibiotic treatments at the farms. We observed that the intestinal contents of individual farmed fish had their own resistome compositions. Our result also showed that the total relative abundances of transposases and tet genes were significantly correlated ( p = 0.001, R 2 = 0.71). In addition, we analyzed the mucosal skin and gill filament resistomes of the farmed fish but only one multidrug-efflux resistance gene ( emrB ) was detected. To our knowledge, this is the first study reporting the resistome of farmed fish using a culture-independent method. Determining the possible sources of

  15. Fun on the Farm: Evaluation of a Lesson to Teach Students about the Spread of Infection on School Farm Visits

    PubMed Central

    Hawking, Meredith K. D.; Lecky, Donna M.; Verlander, Neville Q.; McNulty, Cliodna A. M.

    2013-01-01

    Background School visits to farms are a positive educational experience but pose risks due to the spread of zoonotic infections. A lesson plan to raise awareness about microbes on the farm and preventative behaviours was developed in response to the Griffin Investigation into the E. coli outbreak associated with Godstone Farm in 2009. This study evaluated the effectiveness of the delivery of the lesson plan in increasing knowledge about the spread of infection on the farm, amongst school students. Methods Two hundred and twenty-five 9–11 year old students from seven junior schools in England participated. Two hundred and ten students filled in identical questionnaires covering microbes, hand hygiene, and farm hygiene before and after the lesson. Statistical analysis assessed knowledge change using difference in percentage correct answers. Results Significant knowledge improvement was observed for all sections. In the ‘Farm Hygiene’ section, girls and boys demonstrated 18% (p<0.001) and 11% (p<0.001) improvement, respectively (girls vs. boys p<0.004). As girls had lower baseline knowledge the greater percentage improvement resulted in similar post intervention knowledge scores between genders (girls 80%, boys 83%). Conclusions The lesson plan was successful at increasing awareness of microbes on the farm and infection prevention measures and should be used by teachers in preparation for a farm visit. PMID:24146765

  16. DairyWise, a whole-farm dairy model.

    PubMed

    Schils, R L M; de Haan, M H A; Hemmer, J G A; van den Pol-van Dasselaar, A; de Boer, J A; Evers, A G; Holshof, G; van Middelkoop, J C; Zom, R L G

    2007-11-01

    A whole-farm dairy model was developed and evaluated. The DairyWise model is an empirical model that simulated technical, environmental, and financial processes on a dairy farm. The central component is the FeedSupply model that balanced the herd requirements, as generated by the DairyHerd model, and the supply of homegrown feeds, as generated by the crop models for grassland and corn silage. The output of the FeedSupply model was used as input for several technical, environmental, and economic submodels. The submodels simulated a range of farm aspects such as nitrogen and phosphorus cycling, nitrate leaching, ammonia emissions, greenhouse gas emissions, energy use, and a financial farm budget. The final output was a farm plan describing all material and nutrient flows and the consequences on the environment and economy. Evaluation of DairyWise was performed with 2 data sets consisting of 29 dairy farms. The evaluation showed that DairyWise was able to simulate gross margin, concentrate intake, nitrogen surplus, nitrate concentration in ground water, and crop yields. The variance accounted for ranged from 37 to 84%, and the mean differences between modeled and observed values varied between -5 to +3% per set of farms. We conclude that DairyWise is a powerful tool for integrated scenario development and evaluation for scientists, policy makers, extension workers, teachers and farmers.

  17. Social environments, risk-taking and injury in farm adolescents.

    PubMed

    Pickett, William; Berg, Richard L; Marlenga, Barbara

    2017-12-01

    Farm environments are especially hazardous for young people. While much is known about acute physical causes of traumatic farm injury, little is known about social factors that may underlie their aetiology. In a nationally representative sample of young Canadians aged 11-15 years, we described and compared farm and non-farm adolescents in terms of the qualities of their social environments, engagement in overt multiple risk-taking as well as how such exposures relate aetiologically to their reported injury experiences. Cross-sectional analysis of survey reports from the 2014 (Cycle 7) Canadian Health Behaviour in School-Aged Children study was conducted. Children (n=2567; 2534 weighted) who reported living or working on farms were matched within schools in a 1:1 ratio with children not living or working on farms. Scales examining quality of social environments and overt risk-taking were compared between the two groups, stratified by gender. We then related the occurrence of any serious injury to these social exposures in direct and interactive models. Farm and non-farm children reported social environments that were quite similar, with the exception of overt multiple risk-taking, which was demonstrably higher in farm children of both genders. Engagement in overt risk-taking, but not the other social environmental factors, was strongly and consistently associated with risks for serious injury in farm as well as non-farm children, particularly among males. Study findings highlight the strength of associations between overt multiple risk-taking and injury among farm children. This appears to be a normative aspect of adolescent farm culture. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/.

  18. Silicon: Potential to Promote Direct and Indirect Effects on Plant Defense Against Arthropod Pests in Agriculture.

    PubMed

    Reynolds, Olivia L; Padula, Matthew P; Zeng, Rensen; Gurr, Geoff M

    2016-01-01

    Silicon has generally not been considered essential for plant growth, although it is well recognized that many plants, particularly Poaceae, have substantial plant tissue concentrations of this element. Recently, however, the International Plant Nutrition Institute [IPNI] (2015), Georgia, USA has listed it as a "beneficial substance". This reflects that numerous studies have now established that silicon may alleviate both biotic and abiotic stress. This paper explores the existing knowledge and recent advances in elucidating the role of silicon in plant defense against biotic stress, particularly against arthropod pests in agriculture and attraction of beneficial insects. Silicon confers resistance to herbivores via two described mechanisms: physical and biochemical/molecular. Until recently, studies have mainly centered on two trophic levels; the herbivore and plant. However, several studies now describe tri-trophic effects involving silicon that operate by attracting predators or parasitoids to plants under herbivore attack. Indeed, it has been demonstrated that silicon-treated, arthropod-attacked plants display increased attractiveness to natural enemies, an effect that was reflected in elevated biological control in the field. The reported relationships between soluble silicon and the jasmonic acid (JA) defense pathway, and JA and herbivore-induced plant volatiles (HIPVs) suggest that soluble silicon may enhance the production of HIPVs. Further, it is feasible that silicon uptake may affect protein expression (or modify proteins structurally) so that they can produce additional, or modify, the HIPV profile of plants. Ultimately, understanding silicon under plant ecological, physiological, biochemical, and molecular contexts will assist in fully elucidating the mechanisms behind silicon and plant response to biotic stress at both the bi- and tri-trophic levels.

  19. Silicon: Potential to Promote Direct and Indirect Effects on Plant Defense Against Arthropod Pests in Agriculture

    PubMed Central

    Reynolds, Olivia L.; Padula, Matthew P.; Zeng, Rensen; Gurr, Geoff M.

    2016-01-01

    Silicon has generally not been considered essential for plant growth, although it is well recognized that many plants, particularly Poaceae, have substantial plant tissue concentrations of this element. Recently, however, the International Plant Nutrition Institute [IPNI] (2015), Georgia, USA has listed it as a “beneficial substance”. This reflects that numerous studies have now established that silicon may alleviate both biotic and abiotic stress. This paper explores the existing knowledge and recent advances in elucidating the role of silicon in plant defense against biotic stress, particularly against arthropod pests in agriculture and attraction of beneficial insects. Silicon confers resistance to herbivores via two described mechanisms: physical and biochemical/molecular. Until recently, studies have mainly centered on two trophic levels; the herbivore and plant. However, several studies now describe tri-trophic effects involving silicon that operate by attracting predators or parasitoids to plants under herbivore attack. Indeed, it has been demonstrated that silicon-treated, arthropod-attacked plants display increased attractiveness to natural enemies, an effect that was reflected in elevated biological control in the field. The reported relationships between soluble silicon and the jasmonic acid (JA) defense pathway, and JA and herbivore-induced plant volatiles (HIPVs) suggest that soluble silicon may enhance the production of HIPVs. Further, it is feasible that silicon uptake may affect protein expression (or modify proteins structurally) so that they can produce additional, or modify, the HIPV profile of plants. Ultimately, understanding silicon under plant ecological, physiological, biochemical, and molecular contexts will assist in fully elucidating the mechanisms behind silicon and plant response to biotic stress at both the bi- and tri-trophic levels. PMID:27379104

  20. The School Farm--A Practical Facility

    ERIC Educational Resources Information Center

    Puckett, James D.

    1977-01-01

    This description of the development and operation of a school farm, by the William Campbell High School in Virginia, includes suggestions for planning and management of a school farm set up for teaching agricultural principles. (TA)