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Sample records for ja ld mai

  1. LD in AD 2000.

    ERIC Educational Resources Information Center

    Smith, Bert Kruger

    The author discusses potential problems and benefits for learning disabled (LD) students in the year 2000. Considered are developments in three areas: human engineering (such as the role of amniocentesis in prevention of disabilities), education (including new audiovisual technology and a restructuring of secondary education), and human…

  2. Engineering Synthetic Antibody Inhibitors Specific for LD2 or LD4 Motifs of Paxillin.

    PubMed

    Nocula-Lugowska, Malgorzata; Lugowski, Mateusz; Salgia, Ravi; Kossiakoff, Anthony A

    2015-07-31

    Focal adhesion protein paxillin links integrin and growth factor signaling to actin cytoskeleton. Most of paxillin signaling activity is regulated via leucine-rich LD motifs (LD1-LD5) located at the N-terminus. Here, we demonstrate a method to engineer highly selective synthetic antibodies (sABs) against LD2 and LD4 that are binding sites for focal adhesion kinase (FAK) and other proteins. Phage display selections against peptides were used to generate sABs recognizing each LD motif. In the obtained X-ray crystal structures of the LD-sAB complexes, the LD motifs are helical and bind sABs through a hydrophobic side, similarly as in the structures with natural paxillin partners. The sABs are capable of pulling down endogenous paxillin in complex with FAK and can visualize paxillin in focal adhesions in cells. They were also used as selective inhibitors to effectively compete with focal adhesion targeting domain of FAK for the binding to LD2 and LD4. The sABs are tools for investigation of paxillin LD binding "platforms" and are capable of inhibiting paxillin interactions, thereby useful as potential therapeutics in the future.

  3. Secondary and College LD Bypass Strategies.

    ERIC Educational Resources Information Center

    Mosby, Robert J.

    1981-01-01

    The author describes the Developmental By-Pass (DBP) Instructional technology for teaching secondary and college learning disabled (LD) students by allowing students to bypass ordering and organizational deficits. (SB)

  4. OTV aeroassist with low L/D

    NASA Astrophysics Data System (ADS)

    Willcockson, W. H.

    1986-10-01

    In an attempt to make the OTV efficient and cost-effective, consideration is given to the aerobraking portion of the mission. It is proposed that the best method for controlling the trajectory in this phase is through the use of a lifting brake. Entry error analysis is used to derive an L/D requirement of 0.12; in addition, a predictor-corrector guidance scheme is developed to control exit apogee and orbital plane geometry in the aeroassist. The guidance includes density feedback functions to compensate for the large atmospheric fluctuations observed in Shuttle entries.

  5. Effect of CAI on Achievement of LD Students in English

    ERIC Educational Resources Information Center

    Sivaram, R. T.; Ramar, R.

    2014-01-01

    The present experimental study was undertaken with three objectives in view, (i) to identify students with language learning disabilities (ii) to develop CAI software to teach LD students through computer-assisted instruction and (iii) to measure the effectiveness of CAI with special reference to LD students. Two matched groups of LD students were…

  6. 7 CFR 1737.32 - Loan Design (LD).

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... Studies-Area Coverage Survey and Loan Design § 1737.32 Loan Design (LD). (a) A loan application requires... studies necessary for the LD, it should be prepared by a competent experienced telecommunications engineer. While the LD is subject to RUS approval, the borrower's selection of an engineer to perform preloan...

  7. Social Information Processing and Emotional Understanding in Children with LD

    ERIC Educational Resources Information Center

    Bauminger, Nirit; Edelsztein, Hany Schorr; Morash, Janice

    2005-01-01

    The present study aimed to comprehensively examine social cognition processes in children with and without learning disabilities (LD), focusing on social information processing (SIP) and complex emotional understanding capabilities such as understanding complex, mixed, and hidden emotions. Participants were 50 children with LD (age range 9.4-12.7;…

  8. Tutoring Strategies for LD College Students' Common Writing Errors.

    ERIC Educational Resources Information Center

    deBeer, Liz

    Despite the repeated adage that "no two LD (learning disabled) students are alike, it is not only possible but important to focus on the most common errors of LD college writers in order to learn how best to serve these students. There are in fact two main categories of these students: severely learning disabled and classically learning…

  9. L(d,2,1)-labeling of sun graphs

    NASA Astrophysics Data System (ADS)

    Indriati, Diari; Martini, Titin S.; Herlinawati, Novita

    2014-03-01

    For positive integer d, L(d,2,1)-labeling of a graph G is a function f from V(G) to the positive integers, f:V(G)→{1,2,...} such that |f(u)-f(v)|≥d if the distance between any 2 vertices u and v is 1 (D(u,v) = 1), |f(u)-f(v)|≥2 if D(u,v) = 2, and |f(u)-f(v)|≥1 if D(u,v) = 3. The L(d,2,1)-labeling number kd(G) of a graph G is the smallest positive integer kd such that G has an L(d,2,1)-labeling with kd as the maximum label. This paper presents a general kd-value of sun graphs Sn for any d ≥ 3 and n ≥ 3.

  10. LD's Expressions of Anxiety in Terms of Minor Somatic Complaints.

    ERIC Educational Resources Information Center

    Margalit, M.; Raviv, A.

    1984-01-01

    Prevalence of minor somatic complaints for learning-disabled children (N=30) was compared to that of two control groups: normal (N=559) and educable mentally retarded children (N=69). LD Ss demonstrated a significantly higher prevalence of complaints, but the extent of resulting absenteeism was less than that of nonhandicapped children. (Author/CL)

  11. Visualization of Learning Scenarios with UML4LD

    ERIC Educational Resources Information Center

    Laforcade, Pierre

    2007-01-01

    Present Educational Modelling Languages are used to formally specify abstract learning scenarios in a machine-interpretable format. Current tooling does not provide teachers/designers with some graphical facilities to help them in reusing existent scenarios. They need human-readable representations. This paper discusses the UML4LD experimental…

  12. Mai-Mai militia and sexual violence in Democratic Republic of the Congo.

    PubMed

    Guy, Kitwe Mulunda

    2014-01-01

    This article sets the reasons for the brutal violence against women. It focuses on three field sites providing insight into Mai-Mai motivations and their attitudes toward sexual violence. According to most sources, 5.5 million people have died since the beginning of the war in the Democratic Republic of the Congo (DRC) in 1994, and rape is used as a weapon of destruction. More than 15,000 rapes were reported in the DRC in the last year--accounts of these rapes include descriptions of horrific acts, such as mutilation and the killing of unborn children. The sexual violence is so severe in the DRC that some have described rape in the country as the worst in the world. Sexual violence has long lasting consequences and far-reaching impacts on individual survivors, their families, and their communities in the Democratic Republic of the Congo.

  13. Isolation of Cryptococcus gattii from a Castanopsis argyrophylla tree hollow (Mai-Kaw), Chiang Mai, Thailand.

    PubMed

    Khayhan, Kantarawee; Hagen, Ferry; Norkaew, Treepradab; Puengchan, Tanpalang; Boekhout, Teun; Sriburee, Pojana

    2017-04-01

    The pathogenic yeast Cryptococcus gattii was isolated from a tree hollow of a Castanopsis argyrophylla King ex Hook.f. (Fagaceae) in Chiang Mai, Thailand. Molecular characterization with amplified fragment length polymorphism analysis and multi-locus sequence typing showed that this isolate belonged to genotype AFLP4/VGI representing C. gattii sensu stricto. Subsequent comparison of the environmental isolate with those from clinical samples from Thailand showed that they grouped closely together in a single cluster.

  14. AOTV Low L/D Preliminary Aeroheating Design Environment

    NASA Technical Reports Server (NTRS)

    Engel, C. D.

    1983-01-01

    The aerothermal environment to a configuration with a brake face which exhibits a low lift to drag ratio (L/D) of below 0.75 is emphasized. The five times geosynchronous (5 x Geo) orbit entry was selected as the design trajectory. The available data base and math model is discussed. The resulting preliminary design environment is documented. Recommendations as to how the design environment may be improved through technological advances are given.

  15. High-efficiency perovskite solar cells prepared by using a sandwich structure MAI-PbI2-MAI precursor film.

    PubMed

    Zhang, Xuhui; Ye, Jiajiu; Zhu, Liangzheng; Zheng, Haiying; Liu, Guozhen; Liu, Xuepeng; Duan, Bin; Pan, Xu; Dai, Songyuan

    2017-04-06

    Two-step deposition has been widely used in the perovskite layer preparation for perovskite solar cells due to its attractive morphology controllability. However, the limited diffusivity of CH3NH3I (MAI) might cause some PbI2 to remain in the perovskite film. The residual PbI2 in the perovskite film would lead to inferior performance of devices, such as, low power conversion efficiency (PCE), poor reproducibility and weak air stability. In this work, we developed a sandwich structure MAI-PbI2-MAI precursor film to prepare a PbI2-free CH3NH3PbI3 perovskite film. In comparison to the two-step approach, the MAI-PbI2-MAI precursor film with a typical sandwich structure formed a uniform and pinhole-free perovskite film without any PbI2 residue, which could significantly improve the performance of the devices. Moreover, the bottom MAI layer of the MAI-PbI2-MAI precursor film could improve the interfacial contact of the porous TiO2 layer, leading to the promotion of the charge transfer and reduction of the recombination rate. Therefore, the devices fabricated from the sandwich structure MAI-PbI2-MAI precursor films showed dramatic improvements of open-circuit voltage (Voc), short-circuit current density (Jsc), fill factor (FF) and PCE. As a result, a promising PCE of 17.8% with good long-term air stability was achieved for the MAI-PbI2-MAI precursor film based PSC, which is better than that prepared by a two-step approach.

  16. The caspase-independent algorithm of programmed cell death in Leishmania induced by baicalein: the role of LdEndoG, LdFEN-1 and LdTatD as a DNA 'degradesome'.

    PubMed

    BoseDasgupta, S; Das, B B; Sengupta, S; Ganguly, A; Roy, A; Dey, S; Tripathi, G; Dinda, B; Majumder, H K

    2008-10-01

    In the post-genomic perspective, the quest of programmed cell death (PCD) mechanisms in kinetoplastid parasites lies in the identification and characterization of cell death executer proteins. Here, we show that baicalein (BLN), a potent topoisomerase IB inhibitor, generates an oxidative stress in the parasites leading to altered physiological and morphological parameters, which are characteristic of PCD. For the first time we elucidate that, caspase-independent activation of a novel effector molecule, endonuclease G (LdEndoG), mediates BLN-induced cell death. Functional characterization of LdEndoG identifies Flap endonuclease-1 (LdFEN-1) and LdTatD-like nuclease as other effector molecules. BLN treatment translocates LdEndoG from mitochondria to nucleus, where it forms separate complexes with LdFEN-1 and LdTatD to constitute a DNA 'degradesome' unique to these parasites. Conditional antisense knockdown of LdEndoG provides protection against PCD. This knowledge paves the path toward a better understanding of the PCD pathway in simpler systems, which could be exploited in anti-leishmanial chemotherapy.

  17. College Writing Labs: Are They Meeting the Needs of Students with LD?

    ERIC Educational Resources Information Center

    Scott, Sally S.

    1991-01-01

    Guidelines are offered for serving learning-disabled (LD) students in college writing labs. LD student characteristics identified by clinical observation and data-based research are reported for spelling, cohesion/coherence, and syntactic complexity/maturity. LD writers are distinguished from basic writers. Current practice in writing centers is…

  18. Understanding School Dropout for Urban, Ethnic Minority Teenage Mothers with Learning Disabilities (LD). Disability Research Brief

    ERIC Educational Resources Information Center

    Taylor-Ritzler, Tina; Balcazar, Fabricio E.; McDonald, Katherine

    2007-01-01

    Youth who experience either LD or teenage motherhood often drop out of school. Further, about 50% of young girls with LD become mothers by their early 20s compared to 28% of young women in the general population. There is therefore a high likelihood that teenage mothers with LD will drop out of school. Dropping out of school is of concern because…

  19. LD dual-end-pumped CW Tm:YLF laser

    NASA Astrophysics Data System (ADS)

    Chen, Xin-yu; Zhang, Yu; Yu, Yong-ji; Wang, Chao; Jin, Guang-yong

    2013-09-01

    We report on a LD dual-end-pumped 792nm continuous wave operation Tm:YLF laser. Firstly, the rate equation of LD end-pumped CW operation Tm:YLF laser were established, in which the energy transfer upconversion and without energy transfer upconversion under continuous-wave considerate were considered, as well the pump threshold and the slope efficiency of the laser system were analyzed. Simultaneously, the cavity stability condition and the pattern matching of the plano- concave resonator were analyzed according to ABCD Matrix theory. Comparing respectively the laser threshold and the slope efficiency and optical-optical conversion efficiency under circumstances which the output mirror transmittance of 15% and 23%. In addition, the M2 of the output laser were contrasted and analyzed in adjusting the resonator cavity length by using different radius of curvature of the output mirror in 150mm, 200mm and 300mm all in the above case. As the process of thermal lens focal length changing greater than 90mm, it exhibited that the two fundamental modes in the cavity resonator matched well in numerical simulation when the radius of curvature of the output mirror was 300mm, as well the two fundamental modes matched well when it more than 100mm in a certain pump power. We designed a single LD dual-end-pumped continuous wave operation Tm:YLF laser. Using Tm:YLF (3 at.%) crystal for gain medium, which the size was 3×3×14mm3. In experiments, the Tm:YLF laser crystal keeps 291K and the temperature control method is water cooling. The length of the resonator was 135mm when L shape plano-concave resonator was applied, and the radius of curvature output mirror was 300mm, as well as the temperature of the Tm:YLF laser crystal was 291K. The output laser we observed by this system and the central laser wavelength was 1944nm. The threshold power was 8.11W and the highest output power reaches to 4.01W when the totally input pump power was 17W, and the optical conversion efficiency was 23

  20. Vanadium removal from LD converter slag using bacteria and fungi.

    PubMed

    Mirazimi, S M J; Abbasalipour, Z; Rashchi, F

    2015-04-15

    Removal of vanadium from Linz-Donawits (LD) converter slag was investigated by means of three different species of microbial systems: Acidithiobacillus thiooxidans (autotrophic bacteria), Pseudomonas putida (heterotrophic bacteria) and Aspergillus niger (fungi). The bioleaching process was carried out in both one-step and two-step process and the leaching efficiencies in both cases were compared. Formation of inorganic and organic acids during the leaching process caused mobilization of vanadium. In order to reduce toxic effects of the metal species on the above mentioned microorganisms, a prolonged adaptation process was performed. Both bacteria, A. thiooxidans and P. putida were able to remove more than 90% of vanadium at slag concentrations of 1-5 g L(-1) after 15 days. Also, the maximum achievable vanadium removal in the fungal system was approximately 92% at a slag concentration of 1 g L(-1) after 22 days.

  1. Characteristic behaviors of students with LD who have teacher-identified math weaknesses.

    PubMed

    Bryant, D P; Bryant, B R; Hammill, D D

    2000-01-01

    Mathematics learning disabilities (LD) have gained increased attention over the last decade from both researchers and practitioners. A large percentage of students receiving learning disability services experience difficulties with mathematics, but little research has examined the specific mathematics behaviors of students with LD who have teacher-identified math weaknesses. This study examines the literature on mathematics LD and identifies specific behaviors from that body of research for the purpose of determining the extent to which those behaviors are observed in students with LD. Data are presented from observations of 391 special education professionals on 1724 students with LD, 870 of whom had identified math weaknesses and 854 of whom did not. Our results validate the existing literature and provide implications for teachers, researchers, and others interested in studying mathematics LD.

  2. The diagnosis of LD in English learners: is it nondiscriminatory?

    PubMed

    Figueroa, Richard A; Newsome, Patricia

    2006-01-01

    This study examined the operationalization of one of the key reforms initiated by the Education for All Handicapped Children Act of 1975 (U.S. Congress, 1975) and continued through the 2004 reauthorization of the Individuals with Disabilities Education Act (U.S. Congress, 2004)--namely, nondiscriminatory assessment. The original and current specifications in federal law require that tests be selected and administered so as not to be racially, culturally, or sexually discriminatory. The specific dimensions studied here pertain to the nondiscriminatory diagnosis of learning disabilities (LD) in English learners. A checklist of legal and professional guidelines for making assessments of English learners was used to evaluate 19 psychological reports made on English learners as part of the assessment process for special education eligibility in a small, urban elementary school district in California. The results of this study present a fairly compelling profile of how the writers of psychological reports--school psychologists--do not use extant legal or professional guidelines for making nondiscriminatory assessments of bilingual children.

  3. MAIS: An Empirically-Based Intelligent CBI System.

    ERIC Educational Resources Information Center

    Christensen, Dean L.; Tennyson, Robert D.

    The goal of the programmatic research program for the Minnesota Adaptive Instructional System (MAIS), an intelligent computer-assisted instruction system, is to empirically investigate generalizable instructional variables and conditions that improve learning through the use of adaptive instructional strategies. Research has been initiated in the…

  4. Psychometric Approaches to the Identification of LD: IQ and Achievement Scores Are Not Sufficient

    ERIC Educational Resources Information Center

    Francis,David J; Fletcher,Jack M.; Stuebing,Karla K.; Lyon,G. Reid; Shaywitz,Bennett A; Shaywitz,Sally E.

    2005-01-01

    Simulated data were used to demonstrate that groups formed by imposing cut-points based on either discrepancy or low-achievement definitions of learning disabilities (LD) are unstable over time. Similar problems were demonstrated in longitudinal data from the Connecticut Longitudinal Study, where 39% of the children designated as having LD in…

  5. Assistive Technology Use by Students with LD in Postsecondary Education: A Case of Application before Investigation?

    ERIC Educational Resources Information Center

    Holmes, Alana; Silvestri, Robert

    2012-01-01

    An increasing number of students with Learning Disabilities (LD) are enrolling in postsecondary education (PSE). Assistive technology (AT) is often provided to these students to circumvent academic deficits. This article will focus on research at the PSE level and students with LD to (a) identify AT service delivery practices, (b) describe the…

  6. Recognizing Non-Verbal Social Cues Promotes Social Performance in LD Adolescents

    ERIC Educational Resources Information Center

    Greenbank, Alicia; Sharon, Assia

    2013-01-01

    The research examined whether an educational intervention could enhance the ability of learning disabled (LD) adolescents to recognize non-verbal emotional messages and thus their social functioning. Most LD children have problems recognizing non-verbal cues, particularly emotional ones, and have social difficulties. The study examined the…

  7. Discriminative Effectiveness of the Luria-Nebraska Neuropsychological Battery for LD Adolescents.

    ERIC Educational Resources Information Center

    Lewis, Richard D.; Lorion, Raymond P.

    1988-01-01

    Evaluation of the Luria-Nebraska Neuropsychological Battery's utility in distinguishing between 30 male adolescents diagnosed as learning disabled (LD) and 30 matched non-LD students indicated that the battery correctly classified 90 percent of the sample with 6.7 percent false positives and 13.3 percent false negatives. (Author/DB)

  8. Refining Diagnoses: Applying the DC-LD to an Irish Population with Intellectual Disability

    ERIC Educational Resources Information Center

    Felstrom, A.; Mulryan, N.; Reidy, J.; Staines, M.; Hillery, J.

    2005-01-01

    Background: The diagnostic criteria for psychiatric disorders for use with adults with learning disabilities/mental retardation (DC-LD) is a diagnostic tool developed in 2001 to improve upon existing classification systems for adults with learning disability. The aim of this study was to apply the classification system described by the DC-LD to a…

  9. The Relation of LD and Gender with Emotional Intelligence in College Students.

    ERIC Educational Resources Information Center

    Reiff, Henry B.; Hatzes, Nanette M.; Bramel, Michael H.; Gibbon, Thomas

    2001-01-01

    This study examined the relation of learning disabilities (LD) and gender with emotional intelligence (as measured by the Emotional Quotient Inventory) in 128 college students. Analyses indicated significant differences between students with and without LD on stress management and adaptability, between men and women students on interpersonal…

  10. Effects of Low-Dose Mindfulness-Based Stress Reduction (MBSR-ld) on Working Adults

    ERIC Educational Resources Information Center

    Klatt, Maryanna D.; Buckworth, Janet; Malarkey, William B.

    2009-01-01

    Mindfulness-based stress reduction (MBSR) has produced behavioral, psychological, and physiological benefits, but these programs typically require a substantial time commitment from the participants. This study assessed the effects of a shortened (low-dose [ld]) work-site MBSR intervention (MBSR-ld) on indicators of stress in healthy working…

  11. Introducing Algebra through the Graphical Representation of Functions: A Study among LD Students

    ERIC Educational Resources Information Center

    Sauriol, Jennifer

    2013-01-01

    This longitudinal study evaluates the impact of a new Algebra 1 course at a High School for language-based learning-disabled (LD) students. The new course prioritized the teaching of relationship graphs and functions as an introduction to algebra. Across three studies, the dissertation documents and evaluates the progress made by LD high school…

  12. A Nationwide Epidemiologic Modeling Study of LD: Risk, Protection, and Unintended Impact

    ERIC Educational Resources Information Center

    McDermott, Paul A.; Goldberg, Michelle M.; Watkins, Marley W.; Stanley, Jeanne L.; Glutting, Joseph J.

    2006-01-01

    Through multiple logistic regression modeling, this article explores the relative importance of risk and protective factors associated with learning disabilities (LD). A representative national sample of 6- to 17-year-old students (N = 1,268) was drawn by random stratification and classified by the presence versus absence of LD in reading,…

  13. The Social Acceptance of Secondary School Students with Learning Disabilities (LD)

    ERIC Educational Resources Information Center

    Lorger, Teja; Schmidt, Majda; Vukman, Karin Bakracevic

    2015-01-01

    This paper aims to shed light on the level of social acceptance among students with learning disabilities (LD) in various secondary school vocational programs in comparison with their peers without disabilities. Our findings are based on an empirical study that comprised 417 students, of whom 85 were students with LD. Based on sociometric analyses…

  14. Effects of low-dose mindfulness-based stress reduction (MBSR-ld) on working adults.

    PubMed

    Klatt, Maryanna D; Buckworth, Janet; Malarkey, William B

    2009-06-01

    Mindfulness-based stress reduction (MBSR) has produced behavioral, psychological, and physiological benefits, but these programs typically require a substantial time commitment from the participants. This study assessed the effects of a shortened (low-dose [ld]) work-site MBSR intervention (MBSR-ld) on indicators of stress in healthy working adults to determine if results similar to those obtained in traditional MBSR could be demonstrated. Participants were randomized into MBSR-ld and wait-list control groups. Self-reported perceived stress, sleep quality, and mindfulness were measured at the beginning and end of the 6-week intervention. Salivary cortisol was assessed weekly. Significant reductions in perceived stress (p = .0025) and increases in mindfulness (p = .0149) were obtained for only the MBSR-ld group (n = 22). Scores on the global measure of sleep improved for the MBSR-ld group (p = .0018) as well as for the control group (p = .0072; n = 20). Implications and future research are discussed.

  15. Risk and resilience for substance abuse among adolescents and adults with LD.

    PubMed

    Cosden, M

    2001-01-01

    Substance abuse is a major problem affecting the health and well-being of many people in our society. Although anecdotal evidence concerning linkages between learning disabilities (LD) and substance abuse has been common, this association is not well documented or understood. This article reviews the current research on substance abuse for adolescents and adults with LD, interpreting findings within a risk and resilience framework. Integrating these studies with the broader literatures on substance abuse and the needs of individuals with LD, the author develops hypotheses regarding specific risk and protective factors that could affect the occurrence of substance abuse for adolescents and adults with LD. Potential risk factors include a poor understanding of one's disability, a lack of skills for developing peer relationships, and the need for prolonged family support. Future directions for research in relation to prevention of and intervention regarding substance abuse among individuals with LD are discussed.

  16. Increasing incidence of hip fracture in Chiang Mai, Thailand.

    PubMed

    Wongtriratanachai, Prasit; Luevitoonvechkij, Sirichai; Songpatanasilp, Thawee; Sribunditkul, Siripoj; Leerapun, Taninnit; Phadungkiat, Sompant; Rojanasthien, Sattaya

    2013-01-01

    Hip fracture is a major health problem in Thailand. This study attempted to examine the incidence, related factors, and trends of hip fracture in Chiang Mai, Thailand. All hip fracture data among patients aged 50 yr or older were collected from hospitals in Chiang Mai, Thailand from August 1, 2006 to July 3, 2007. Data from the 1997 Chiang Mai hip fracture study were used for comparison. In the study period, 690 hip fractures were reported: 203 males and 487 females (male to female ratio was 1 to 2.4), with a mean age of 76.7 yr. The estimated cumulative incidence was 181.0 per 100,000, and the adjusted incidence was 253.3 (males: 135.9; females: 367.9). A simple fall was the most common mechanism (79%) of fracture, and 80% of the hip fractures occurred in patients aged 70 yr or older. The highest incidence of hip fracture was observed in patients older than 85 yr (1239). At 6 mo postfracture, most patients (61%) used a walking aid. Compared with the 1997 data, hip fracture incidence had increased by an average of 2% per yr, and the incidence of hip fracture had increased significantly from August 1, 2006 to July 31, 2007, especially in patients older than 75 yr. In patients older than 84 yr, the incidence increased by a factor of 2. Urgent strategies for the prevention and treatment of osteoporosis, and hence hip fracture, are needed.

  17. Tunable single frequency fiber laser based on FP-LD injection locking.

    PubMed

    Zhang, Aiqin; Feng, Xinhuan; Wan, Minggui; Li, Zhaohui; Guan, Bai-ou

    2013-05-20

    We propose and demonstrate a tunable single frequency fiber laser based on Fabry Pérot laser diode (FP-LD) injection locking. The single frequency operation principle is based on the fact that the output from a FP-LD injection locked by a multi-longitudinal-mode (MLM) light can have fewer longitudinal-modes number and narrower linewidth. By inserting a FP-LD in a fiber ring laser cavity, single frequency operation can be possibly achieved when stable laser oscillation established after many roundtrips through the FP-LD. Wavelength switchable single frequency lasing can be achieved by adjusting the tunable optical filter (TOF) in the cavity to coincide with different mode of the FP-LD. By adjustment of the drive current of the FP-LD, the lasing modes would shift and wavelength tunable operation can be obtained. In experiment, a wavelength tunable range of 32.4 nm has been obtained by adjustment of the drive current of the FP-LD and a tunable filter in the ring cavity. Each wavelength has a side-mode suppression ratio (SMSR) of at least 41 dB and a linewidth of about 13 kHz.

  18. Individual and combined effects of LD and ADHD on computerized neurocognitive concussion test performance: evidence for separate norms.

    PubMed

    Elbin, R J; Kontos, Anthony P; Kegel, Nate; Johnson, Eric; Burkhart, Scott; Schatz, Philip

    2013-08-01

    Decreased neurocognitive performance in individuals with self-reported attention deficit hyperactivity disorder (ADHD) and learning disability (LD) is well documented in the neuropsychological research literature. Previous studies employing paper-and-pencil neurocognitive assessments report lower performance in individuals with ADHD and LD. The purpose of the current study was to examine the influence of a self-reported diagnosis of LD, ADHD, and combined LD/ADHD on baseline computerized neurocognitive testing (CNT) used for the concussion assessment. Results revealed athletes with a self-reported diagnosis of LD, ADHD, and/or combined LD/ADHD demonstrated lower performance on baseline CNT and reported larger numbers of symptoms than did control athletes without these diagnoses. These findings provide evidence for the development of separate normative data for athletes with LD, ADHD, and LD/ADHD diagnoses on CNT batteries commonly used for concussion management.

  19. LdFlabarin, a New BAR Domain Membrane Protein of Leishmania Flagellum

    PubMed Central

    Thonnus, Magali; Salin, Bénédicte; Boissier, Fanny; Blancard, Corinne; Sauvanet, Cécile; Metzler, Christelle; Espiau, Benoît; Sahin, Annelise; Merlin, Gilles

    2013-01-01

    During the Leishmania life cycle, the flagellum undergoes successive assembly and disassembly of hundreds of proteins. Understanding these processes necessitates the study of individual components. Here, we investigated LdFlabarin, an uncharacterized L. donovani flagellar protein. The gene is conserved within the Leishmania genus and orthologous genes only exist in the Trypanosoma genus. LdFlabarin associates with the flagellar plasma membrane, extending from the base to the tip of the flagellum as a helicoidal structure. Site-directed mutagenesis, deletions and chimera constructs showed that LdFlabarin flagellar addressing necessitates three determinants: an N-terminal potential acylation site and a central BAR domain for membrane targeting and the C-terminal domain for flagellar specificity. In vitro, the protein spontaneously associates with liposomes, triggering tubule formation, which suggests a structural/morphogenetic function. LdFlabarin is the first characterized Leishmania BAR domain protein, and the first flagellum-specific BAR domain protein. PMID:24086735

  20. PLC Software Program for Leak Detector Station A1 SALW-LD-ST-A1

    SciTech Connect

    KOCH, M.R.

    2001-01-25

    This document describes the software program for the programmable logic controller for the leak detector station ''SALW-LD-ST-A1''. The appendices contains a copy of the printout of the software program.

  1. Effectiveness of Steroid/Antibiotic Treatment in Primates Administered LD100 Escherichia coli.

    DTIC Science & Technology

    1981-07-01

    probability for survival of baboons given LD100 E . coli . The present study was designed to determine if baboons would recover when initiation of treatment...was delayed until they had sustained E . coli -induced systemic hypotension for a period of approximately three hours. Sixteen adult baboons were each...administered a two-hour infusion of LD100 E . coli . All eight untreated animals died within 42 hours. Five of the eight baboons treated after

  2. Structure and expression of a mouse major histocompatibility antigen gene, H-2Ld.

    PubMed Central

    Evans, G A; Margulies, D H; Camerini-Otero, R D; Ozato, K; Seidman, J G

    1982-01-01

    A genomic clone encoding H-2Ld, a mouse major transplantation antigen, has been identified and the structure of the H-2Ld gene has been partially determined. We isolated 35 genomic clones from a BALB/c (H-2d) genomic library by hybridization to mouse or human probes. One of these clones encodes H-2Ld as determined by two criteria. First, the gene encodes a protein that is identical at the 76 known amino acid positions for H-2Ld. Second, when introduced into L cells by DNA-mediated gene transfer, a new H-2 antigen is expressed that is recognized by anti-H-2Ld monoclonal antibodies. The sequence of the H-2Ld protein predicted by the DNA sequences shows more than 80% homology to known H-2 antigens. H-2L-like sequences are found in mutant H-2Kb molecules, suggesting that gene conversion or reciprocal recombination may play a role in the development of H-2 polymorphism. PMID:6952248

  3. Engineering and characterization of a stabilized alpha1/alpha2 module of the class I major histocompatibility complex product Ld.

    PubMed

    Jones, Lindsay L; Brophy, Susan E; Bankovich, Alexander J; Colf, Leremy A; Hanick, Nicole A; Garcia, K Christopher; Kranz, David M

    2006-09-01

    The major histocompatibility complex (MHC) is the most polymorphic locus known, with thousands of allelic variants. There is considerable interest in understanding the diversity of structures and peptide-binding features represented by this class of proteins. Although many MHC proteins have been crystallized, others have not been amenable to structural or biochemical studies due to problems with expression or stability. In the present study, yeast display was used to engineer stabilizing mutations into the class I MHC molecule, Ld. The approach was based on previous studies that showed surface levels of yeast-displayed fusion proteins are directly correlated with protein stability. To engineer a more stable Ld, we selected Ld mutants with increased surface expression from randomly mutated yeast display libraries using anti-Ld antibodies or high affinity, soluble T-cell receptors (TCRs). The most stable Ld mutant, Ld-m31, consisted of a single-chain MHC module containing only the alpha1 and alpha2 domains. The enhanced stability was in part due to a single mutation (Trp-97 --> Arg), shown previously to be present in the allele Lq. Mutant Ld-m31 could bind to Ld peptides, and the specific peptide.Ld-m31 complex (QL9.Ld-m31) was recognized by alloreactive TCR 2C. A soluble form of the Ld-m31 protein was expressed in Escherichia coli and refolded from inclusion bodies at high yields. Surface plasmon resonance showed that TCRs bound to peptide.Ld-m31 complexes with affinities similar to those of native full-length Ld. The TCR and QL9.Ld-m31 formed complexes that could be resolved by native gel electrophoresis, suggesting that stabilized alpha1/alpha2 class I platforms may enable various structural studies.

  4. Chemical and mineralogical characterizations of LD converter steel slags: A multi-analytical techniques approach

    SciTech Connect

    Waligora, J.; Bulteel, D.; Degrugilliers, P.; Damidot, D.; Potdevin, J.L.; Measson, M.

    2010-01-15

    The use of LD converter steel slags (coming from Linz-Donawitz steelmaking process) as aggregates in road construction can in certain cases lead to dimensional damage due to a macroscopic swelling that is the consequence of chemical reactions. The aim of this study was to couple several analytical techniques in order to carefully undertake chemical and mineralogical characterizations of LD steel slags and identify the phases that are expected to be responsible for their instability. Optical microscopy, scanning electron microscopy and electron probe microanalyses revealed that LD steel slags mainly contain calcium silicates, dicalcium ferrites, iron oxides and lime. However, as a calcium silicate phase is heterogeneous, Raman microspectrometry and transmitted electron microscopy had to be used to characterize it more precisely. Results showed that lime is present under two forms in slag grains: some nodules observed in the matrix whose size ranges from 20 to 100 {mu}m and some micro-inclusions, enclosed in the heterogeneous calcium silicate phase whose size ranges from 1 to 3 {mu}m. It was also established that without the presence of magnesia, lime is expected to be the only phase responsible for LD steel slags instability. Nevertheless, the distribution of lime between nodules and micro-inclusions may play a major role and could explain that similar amounts of lime can induce different instabilities. Thus, it appears that lime content of LD steel slags is not the only parameter to explain their instability.

  5. Can social stories enhance the interpersonal conflict resolution skills of children with LD?

    PubMed

    Kalyva, Efrosini; Agaliotis, Ioannis

    2009-01-01

    Since many children with learning disabilities (LD) face interpersonal conflict resolution problems, this study examines the efficacy of social stories in helping them choose more appropriate interpersonal conflict resolution strategies. A social story was recorded and played to the 31 children with LD in the experimental group twice a week for a period of 1 month, while the 32 children with LD in the control group did not receive any intervention. The effects of the intervention were systematically examined by means of an interview with the participants, while teachers completed the T-MESSY (Matson, J. L. (1990). Matson Evaluation of Social Skills With Youngsters: Manual. Worthington, OR: International Diagnostic Systems). All children chose mainly avoidance and hostile strategies before the intervention, but children in the experimental group chose predominantly positive strategies both after the intervention and at follow-up in comparison to control children. Furthermore, children with LD who received the intervention were rated by their teachers as engaging in significantly less inappropriate social behaviors after the intervention and at follow-up in comparison to control children. The recorded changes in the choice of interpersonal conflict resolution strategies and the more positive teacher ratings for the experimental group indicate that social stories constitute a powerful intervention for the enhancement of the social competence of children with LD.

  6. Cancer in Chiang Mai, North Thailand. A Relative Frequency Study

    PubMed Central

    Menakanit, W.; Muir, C. S.; Jain, D. K.

    1971-01-01

    The relative frequency of histologically diagnosed cancer at the Chiang Mai Medical School in North Thailand in 1964-67 inclusive is examined. Most of the 1877 cancers seen are in Thais (males, 927; females, 908), the remainder arising in Chinese (males, 21; females, 12), Hill People (males, 6; females, 1) and others (females, 2). The cancers in Thais are presented by site, sex and 10-year age-group together with the relative frequency (crude and corrected for age). In Thais, the outstanding finding is the extraordinarily high frequency of cancer of the hypopharyngeal-laryngeal region in both sexes (males, 18.4 per cent; females, 3.4 per cent). This may be associated with the smoking of a local variety of the cigar called “keeyo” This cigar, smoked in the usual manner, contains approximately equal quantities of home-grown, sun-dried, Thai tobacco and the chopped bark of the “koi” tree (Streblus asper). In women, who also smoke “keeyo”, the frequency of hypopharyngeal-laryngeal cancer is unusually high by occidental standards. There is no sex difference in the frequency of bronchial cancer (4 per cent). In males cancer of the penis, in second rank (6.6 per cent) is much more frequent than cancer of the prostate and testis combined. Stomach and skin cancers (ICD 191) are in third place (each 5.7 per cent). In females the most frequent cancers are cervix uteri (19.8 per cent), breast (8.8 per cent) and skin (ICD 191) (6.1 per cent). Cancers of the lip and skin of the head and neck are more frequent in females than in males. Choriocarcinoma is common (1.9 per cent) and there is a large number of vulvar cancers in young women (2.6 per cent). The geography, economy and medical facilities of Chiang Mai Province are described. It is considered that, although there is likely to be considerable under-reporting of internal cancers, the high frequency of hypopharyngeallaryngeal cancer is not due to selective bias. ImagesFig. 2 PMID:5165581

  7. Theoretical analysis of the semi-ring and trapezoid LD side-pumped alkali vapor lasers

    NASA Astrophysics Data System (ADS)

    Shen, Binglin; Xu, Xingqi; Xia, Chunsheng; Pan, Bailiang

    2016-12-01

    Analysis of two new pump-couplings: semi-ring and trapezoid LD side-pumped configurations in alkali vapor lasers is reported, which mainly includes the numerical approaches for evaluation of the pump intensity and temperature distribution in the cell of these two configurations. Comparison between the simulated results of the semi-ring and trapezoid LD side-pumped Cs vapor lasers and the experimental results of the single-side pumped Cs vapor lasers with a cylindrical white diffuse reflector and a stable or unstable resonator is made. Dependencies of laser power on pump power and flowed velocity for semi-ring, trapezoid, single and double side-pumped configurations are calculated, demonstrating the advantages of the semi-ring and trapezoid LD side-pumped configurations. Thus the model is very helpful for designing high-power side-pumped alkali vapor lasers.

  8. English language learners who struggle with reading: language acquisition or LD?

    PubMed

    Klingner, Janette K; Artiles, Alfredo J; Barletta, Laura Méndez

    2006-01-01

    We review empirical research on English language learners (ELLs) who struggle with reading and who may have learning disabilities (LD). We sought to determine research indicators that can help us better differentiate between ELLs who struggle to acquire literacy because of their limited proficiency in English and ELLs who have actual LD. We conclude that more research is warranted to further elucidate the strengths and learning needs of subgroups of underachieving ELLs, to help us determine who should qualify for special education, and to clarify why some ELLs who do not have LD still struggle with language and literacy acquisition. Future research should account for the complexities involved in becoming literate in another language and focus more on cultural and contextual factors that affect student achievement.

  9. Comorbidity of LD and ADHD: implications of DSM-5 for assessment and treatment.

    PubMed

    DuPaul, George J; Gormley, Matthew J; Laracy, Seth D

    2013-01-01

    Attention-deficit/hyperactivity disorder (ADHD) and learning disability (LD) can co-occur for a significant minority of children with each disorder. A total of 17 studies (2001-2011) examining ADHD-LD comorbidity were reviewed, revealing a higher mean comorbidity rate (45.1%) than has been obtained previously. Higher comorbidity may be the result of including students with writing disorders, not just reading and/or math disabilities. Proposed DSM-5 criteria for both disorders will likely affect comorbidity rates; however, it is unclear whether such rates will increase or decrease. Regardless of the specific impact of DSM revisions, academic skill and/or performance deficits should be assessed for students with ADHD as part of screening, comprehensive evaluation, and treatment monitoring. Comprehensive intervention services for students with comorbid ADHD and LD will require empirically supported treatment strategies that address both disorders and that are implemented across school and home settings.

  10. Self-efficacy from the perspective of adolescents with LD and their specialist teachers.

    PubMed

    Klassen, Robert M; Lynch, Shane L

    2007-01-01

    This study used qualitative methodology to investigate the self-efficacy beliefs of early adolescents with learning disabilities (LD). We conducted a series of focus group interviews with 28 Grade 8 and 9 students with LD and individual interviews with 7 specialist LD teachers. Content analyses of the student and teacher data resulted in 2 a priori and 3 inductive themes: self-efficacy, calibration and levels of self-efficacy, students' self-awareness, attributions for failure, and problems and solutions. The students viewed themselves as low in self-efficacy and generally accurate in the calibration of their efficacy and performance, whereas the teachers viewed the students as overconfident about academic tasks. In contrast to the teachers, the students viewed verbal persuasion as a valued source of self-efficacy. Students attributed their failures to lack of effort, whereas their teachers attributed student failure to uncontrollable deficits. Problems and solutions related to student motivation were discussed from student and teacher perspectives.

  11. A Dynamic Simulation Model of the Management Accounting Information Systems (MAIS)

    NASA Astrophysics Data System (ADS)

    Konstantopoulos, Nikolaos; Bekiaris, Michail G.; Zounta, Stella

    2007-12-01

    The aim of this paper is to examine the factors which determine the problems and the advantages on the design of management accounting information systems (MAIS). A simulation is carried out with a dynamic model of the MAIS design.

  12. Knockdown of a nutrient amino acid transporter gene LdNAT1 reduces free neutral amino acid contents and impairs Leptinotarsa decemlineata pupation

    PubMed Central

    Fu, Kai-Yun; Guo, Wen-Chao; Ahmat, Tursun; Li, Guo-Qing

    2015-01-01

    A Leptinotarsa decemlineata SLC6 NAT gene (LdNAT1) was cloned. LdNAT1 was highly expressed in the larval alimentary canal especially midgut. LdNAT1 mRNA levels were high right after the molt and low just before the molt. JH and a JH analog pyriproxyfen activated LdNAT1 expression. RNAi of an allatostatin gene LdAS-C increased JH and upregulated LdNAT1 transcription. Conversely, silencing of a JH biosynthesis gene LdJHAMT decreased JH and reduced LdNAT1 expression. Moreover, 20E and an ecdysteroid agonist halofenozide repressed LdNAT1 expression, whereas a decrease in 20E by RNAi of an ecdysteroidogenesis gene LdSHD and disruption of 20E signaling by knockdown of LdE75 and LdFTZ-F1 activated LdNAT1 expression. Thus, LdNAT1 responded to both 20E and JH. Moreover, knockdown of LdNAT1 reduced the contents of cysteine, histidine, isoleucine, leucine, methionine, phenylalanine and serine in the larval bodies and increased the contents of these amino acids in the larval feces. Furthermore, RNAi of LdNAT1 inhibited insulin/target of rapamycin pathway, lowered 20E and JH titers, reduced 20E and JH signaling, retarded larval growth and impaired pupation. These data showed that LdNAT1 was involved in the absorption of several neutral amino acids critical for larval growth and metamorphosis. PMID:26657797

  13. 1059 and 1328nm LD pumped Nd:S-FAP solid state laser

    SciTech Connect

    Sun Lianke; Zhang Shaojun; Zhao Shengzhi; Wang Qingpu

    1996-12-31

    In this paper the authors introduce a new laser crystal--Nd{sup 3+}:Sr{sub 5}(PO{sub 4}){sub 3}F, Nd:S-FAP, and present its optical and physical characteristics. Based on the experiment lasing performance of CW LD pumped Nd:S-FAP crystal is reported here: the threshold and slope efficiency of 1059 nm Nd:S-FAP laser pumped by CW LD at 805nm are 7mW and 41%, and that of 1328nm Nd:S-FAP laser are 19mW and 35%. The comparison between experimental result and theoretical calculation is also discussed in this paper.

  14. Pluralizing Methodologies in the Field of LD: From "What Works" to What Matters

    ERIC Educational Resources Information Center

    Ferri, Beth A.; Gallagher, Deborah; Connor, David J.

    2011-01-01

    The field of learning disabilities (LD) has a complex and complicated history. Tensions over definitions, eligibility criteria, service delivery models, and best practices, as well as epistemological debates, have been a part of that history from its inception. Given our collective struggles, as well as the current realities facing the field,…

  15. The End of Innocence: Historiography and Representation in the Discursive Practice of LD

    ERIC Educational Resources Information Center

    Artiles, Alfredo J.

    2004-01-01

    In this article, I discuss two interrelated sets of challenges that the discursive practice of learning disability (LD) will need to address, namely, issues associated with the development of a historiography of special education and a more complex understanding of representation issues. I use social theory to address these challenges and raise…

  16. Individual and Longitudinal Differences among High and Low-Achieving, LD, and ADHD L2 Learners

    ERIC Educational Resources Information Center

    Sparks, Richard L.; Humbach, Nancy; Javorsky, James

    2008-01-01

    High-achieving (HA) and low-achieving (LA), learning disabled (LD), and attention deficit hyperactivity disorder (ADHD) high school students were followed over two years of L2 study and compared on measures of L1 literacy (reading and writing) in elementary school, L1 cognitive ability, L2 aptitude, oral and written L2 proficiency, and L2 word…

  17. The College-Bound L.D. Student--Let's Bury the Myths Forever.

    ERIC Educational Resources Information Center

    DeDecker, Sally

    This paper addresses myths about college students with learning disabilities (LD) which may give false hope to students very unlikely to succeed in college but more often place limitations on students who could succeed with some support services, based on the experience of Project Achieve at Southern Illinois University. The "myths"…

  18. Perception of Nonverbal Social Cues by Regular Education, ADHD, and ADHD/LD Students.

    ERIC Educational Resources Information Center

    Hall, Cathy W.; Peterson, Andrea D.; Webster, Raymond E.; Bolen, Larry M.; Brown, Michael B.

    1999-01-01

    Study examined ability of attention deficit hyperactive disorder (ADHD) children with and without learning disability to perceive nonverbal social cues. ADHD/LD children demonstrated significant difficulty in comparison to their peers in effectively perceiving paralanguage cues. This group also showed significant improvement on the Postures and…

  19. The acute lethal dose 50 (LD50) of caffeine in albino rats.

    PubMed

    Adamson, Richard H

    2016-10-01

    An acute LD50 is a statistically derived amount of a substance that can be expected to cause death in 50% of the animals when given by a specified route as a single dose and the animals observed for a specified time period. Although conducting routine acute toxicity testing in rodents has been criticized, it can serve useful functions and also have practical implications. Material safety data sheets (MSDS) will reflect the acute toxicity of a substance and may require workers to wear protective gear, if appropriate, based on the LD50. There is no information in the scientific published literature which calculates a mean LD50 and standard deviation for caffeine administered orally to rats, using studies performed under good laboratory practice (GLP) or equivalent. This report does that and should be useful to manufacturers, packagers, transporters and regulators of this material. Using data from studies that are reproducible and reliable, the most accurate estimate of the acute LD50 of caffeine administered orally in male albino rats is hereby reported to be 367/mg/kg.

  20. The Impact of the Flynn Effect on LD Diagnoses in Special Education

    ERIC Educational Resources Information Center

    Kanaya, Tomoe; Ceci, Stephen

    2012-01-01

    Because of the Flynn effect, IQ scores rise as a test norm ages but drop on the introduction of a newly revised test norm. The purpose of the current study was to determine the impact of the Flynn effect on learning disability (LD) diagnoses, the most prevalent special education diagnosis in the United States. Using a longitudinal sample of 875…

  1. Perspectives on Inclusion: Students with LD, Their Parents, and Their Teachers

    ERIC Educational Resources Information Center

    Chmiliar, Linda

    2009-01-01

    One of the most significant changes in education over the last few decades is the movement toward inclusive education. Many schools have adopted an inclusive approach of educating students with learning disabilities (LD) in the regular classroom. The present study utilized a multiple case study approach to examine the perspectives of stakeholders…

  2. Mathematics Interventions for Students with Learning Disabilities (LD) in Secondary School: A Review of the Literature

    ERIC Educational Resources Information Center

    Myers, Jonté A.; Wang, Jun; Brownell, Mary T.; Gagnon, Joseph Calvin

    2015-01-01

    The purpose of our literature review was to extend and update Maccini, Mulcahy, and Wilson's (2007) review of the literature on mathematics interventions for secondary students with learning disabilities (LD). An extensive search of the research literature netted 15 research studies that focused on mathematics interventions for secondary students…

  3. Practices and Attitudes of Postsecondary LD Service Providers in North America.

    ERIC Educational Resources Information Center

    Yost, Deborah S.; And Others

    1994-01-01

    A nationwide survey of 510 postsecondary learning disability (LD) service providers revealed that an educationally diverse group of professionals is developing programs and providing direct instruction to students with learning disabilities. Service providers are encouraged to utilize interventions that lead to self-determination and independence…

  4. A Review of Story Mapping Instruction for Secondary Students with LD

    ERIC Educational Resources Information Center

    Boon, Richard T.; Paal, Michael; Hintz, Anna-Maria; Cornelius-Freyre, Melissa

    2015-01-01

    The purpose of this article is to provide a review on the effectiveness of story mapping to improve the reading comprehension skills of middle and high school (Grades 6-12) students with learning disabilities (LD). An extensive review of the special education research-base revealed twelve (N = 12) story mapping intervention studies that met our…

  5. Use of the adaptive classifier for determination of LD50 in the acute radiation disease.

    PubMed

    Vodicka, I; Hanus, J; Hradil, J

    1989-01-01

    In experiments on female Wistar rats a new method for the determination of LD50 is demonstrated and compared with the classical probit method using the same experimental animals. The method is applicable for the computation of LD50 and analogical quantities in man, too. The method is based on the application of an adaptive logical circuit (ADALINE) trained for the dichotomous prognostic classification of irradiated individuals quod vitam according to a set of clinical and laboratory indicators registered on the third day after irradiation. After the training procedure has been finished, the classifier makes possible an individual prognosis of survival or death. The analogue output signal according to which the classification is performed changes continually from negative to positive values and exhibits S-shaped relation to the radiation dose. Its zero value corresponds to the position of LD50 on the abscissa. For the construction of the searched function, i.e. for the optimum approximation of experimentally obtained values of the output signal, the method of the changeable polyhedron was applied belonging to the optimalization numerical methods used in the regulation technics. The computed value of LD50 was 7.80 Gy in rats very closely corresponding with the value 7.61 Gy determined by means of the classical probit method.

  6. Differential Diagnosis of Dysgraphia, Dyslexia, and OWL LD: Behavioral and Neuroimaging Evidence.

    PubMed

    Berninger, Virginia W; Richards, Todd; Abbott, Robert D

    2015-10-01

    In Study 1, children in grades 4 to 9 (N= 88, 29 females and 59 males) with persisting reading and/or writing disabilities, despite considerable prior specialized instruction in and out of school, were given an evidence-based comprehensive assessment battery at the university while parents completed questionnaires regarding past and current history of language learning and other difficulties. Profiles (patterns) of normed measures for different levels of oral and written language used to categorize participants into diagnostic groups for dysgraphia (impaired subword handwriting) (n=26), dyslexia (impaired word spelling and reading) (n=38), or oral and written language learning disability OWL LD (impaired oral and written syntax comprehension and expression) (n=13) or control oral and written language learners (OWLs) without SLDs (n=11) were consistent withreported history. Impairments in working memory components supporting language learning were also examined. In Study 2, right handed children from Study 1 who did not wear braces (controls, n=9, dysgraphia, n= 14; dyslexia, n=17, OWL LD, n=5) completed an fMRI functional connectivity brain imaging study in which they performed a word-specific spelling judgment task, which is related to both word reading and spelling, and may be impaired in dysgraphia, dyslexia, and OWL LD for different reasons. fMRI functional connectivity from 4 seed points in brain locations involved in written word processing to other brain regions also differentiated dysgraphia, dyslexia, and OWL LD; both specific regions to which connected and overall number of functional connections differed. Thus, results provide converging neurological and behavioral evidence, for dysgraphia, dyslexia, and OWL LD being different, diagnosable specific learning disabilities (SLDs) for persisting written language problems during middle childhood and early adolescence. Translation of the research findings into practice at policy and administrative levels and

  7. Oxidative stress and apoptosis in a pig model of brain death (BD) and living donation (LD)

    PubMed Central

    2013-01-01

    Background As organ shortage is increasing, the acceptance of marginal donors increases, which might result in poor organ function and patient survival. Mostly, organ damage is caused during brain death (BD), cold ischemic time (CIT) or after reperfusion due to oxidative stress or the induction of apoptosis. The aim of this study was to study a panel of genes involved in oxidative stress and apoptosis and compare these findings with immunohistochemistry from a BD and living donation (LD) pig model and after cold ischemia time (CIT). Methods BD was induced in pigs; after 12 h organ retrieval was performed; heart, liver and kidney tissue specimens were collected in the BD (n = 6) and in a LD model (n = 6). PCR analysis for NFKB1, GSS, SOD2, PPAR-alpha, OXSR1, BAX, BCL2L1, and HSP 70.2 was performed and immunohistochemistry used to show apoptosis and nitrosative stress induced cell damage. Results In heart tissue of BD BAX, BCL2L1 and HSP 70.2 increased significantly after CIT. Only SOD2 was over-expressed after CIT in BD liver tissue. In kidney tissue, BCL2L1, NFKB, OXSR1, SOD2 and HSP 70.2 expression was significantly elevated in LD. Immunohistochemistry showed a significant increase in activated Caspase 3 and nitrotyrosine positive cells after CIT in BD in liver and in kidney tissue but not in heart tissue. Conclusion The up-regulation of protective and apoptotic genes seems to be divergent in the different organs in the BD and LD setting; however, immunohistochemistry revealed more apoptotic and nitrotyrosine positive cells in the BD setting in liver and kidney tissue whereas in heart tissue both BD and LD showed an increase. PMID:24088575

  8. The Leishmania donovani histidine acid ecto-phosphatase LdMAcP: insight into its structure and function

    PubMed Central

    Papadaki, Amalia; Politou, Anastasia S.; Smirlis, Despina; Kotini, Maria P.; Kourou, Konstadina; Papamarcaki, Thomais; Boleti, Haralabia

    2015-01-01

    Acid ecto-phosphatase activity has been implicated in Leishmania donovani promastigote virulence. In the present study, we report data contributing to the molecular/structural and functional characterization of the L. donovani LdMAcP (L. donovani membrane acid phosphatase), member of the histidine acid phosphatase (HAcP) family. LdMAcP is membrane-anchored and shares high sequence identity with the major secreted L. donovani acid phosphatases (LdSAcPs). Sequence comparison of the LdMAcP orthologues in Leishmania sp. revealed strain polymorphism and species specificity for the L. donovani complex, responsible for visceral leishmaniasis (Khala azar), proposing thus a potential value of LdMAcP as an epidemiological or diagnostic tool. The extracellular orientation of the LdMAcP catalytic domain was confirmed in L. donovani promastigotes, wild-type (wt) and transgenic overexpressing a recombinant LdMAcP–mRFP1 (monomeric RFP1) chimera, as well as in transiently transfected mammalian cells expressing rLdMAcP–His. For the first time it is demonstrated in the present study that LdMAcP confers tartrate resistant acid ecto-phosphatase activity in live L. donovani promastigotes. The latter confirmed the long sought molecular identity of at least one enzyme contributing to this activity. Interestingly, the L. donovani rLdMAcP–mRFP1 promastigotes generated in this study, showed significantly higher infectivity and virulence indexes than control parasites in the infection of J774 mouse macrophages highlighting thereby a role for LdMAcP in the parasite's virulence. PMID:25695743

  9. Profiles of Elementary-Age English Language Learners with Reading-Related Learning Disabilities (LD) Identified as Speech and Language Impaired Prior to, at, or after Identification as LD

    ERIC Educational Resources Information Center

    McGhee, Belinda Maria Despujols

    2011-01-01

    This study examined the characteristics of 14 English Language Learners classified as having learning disabilities (LD) who were also identified as having speech and language impairments (SI) prior to, at, or after initial identification as LD. Data were collected under the auspices of a longitudinal study, Bilingual Exceptional Students:…

  10. Effect of solvents on the spectroscopic properties of LD-489 & LD-473: Estimation of ground and excited state dipole moments by solvatochromic shift method

    NASA Astrophysics Data System (ADS)

    Deepa, H. R.; Thipperudrappa, J.; Suresh Kumar, H. M.

    2013-05-01

    The absorption and fluorescence spectra of 6,7,8,9-tetrahydro-6,8,9-trimethyl-4-(trifluoro methyl)-2H-pyrano[2,3-b][1,8]naphthyridin-2-one (LD-489) and 1,2,3,8-tetrahydro-1,2,3,3,8-pentamethyl-5-(trifluoromethyl)-7H-pyrrolo[3,2-g]quinolin-7-one (LD-473) have been recorded at room temperature in different solvents and 1,4-dioxane-acetonitrile solvent mixtures. The UV-Visible absorption spectra are less sensitive to solvent polarity than the corresponding fluorescence spectra in both the dyes which show pronounced solvatochromic effect. The effects of solvents upon the spectral properties are analyzed using Lippert-Mataga polarity function, Richardts microscopic solvent polarity parameter and Catalán's multiple linear regression approach. Both general solute-solvent interactions and specific interactions are operative in these systems. The solvatochromic correlations are used to estimate excited state dipole moments using experimentally determined ground state dipole moments. The excited state dipole moment for both the dyes are found to be larger than their corresponding ground state dipole moment and is interpreted based on their resonance structures.

  11. Impact of the Social Construction of LD on Culturally Diverse Families: A Response to Reid and Valle

    ERIC Educational Resources Information Center

    Kalyanpur, Maya; Harry, Beth

    2004-01-01

    This response focuses on the effects of the current construction of learning disabilities (LD) on families of children from devalued racial/ethnic groups. Agreeing with the arguments of Reid and Valle, we add that parents from such groups are further disenfranchised because they are not participants in the critical discourse surrounding LD and…

  12. A Synthesis on the Research of the Comorbidity of ADHD and LD in Korea: Perspective and Trend

    ERIC Educational Resources Information Center

    Kim, Dongil; Koh, Eunyoung; Jeong, Sora; Lee, Kijyung; Kim, Boongnyun; Kim, Ienai

    2011-01-01

    Attention-deficit/hyperactivity disorder (ADHD) and learning disabilities (LD) are the most commonly diagnosed childhood disorders, and they frequently co-occur with each other. It has been found that students with comorbidity of ADHD and LD experience more difficulties in school. Even though the research interests in the comorbidity of ADHD and…

  13. Using Prompt Fading to Teach Self-Questioning to Fifth Graders with LD: Effects on Reading Comprehension

    ERIC Educational Resources Information Center

    Rouse, Christina A.; Alber-Morgan, Sheila R.; Cullen, Jennifer M.; Sawyer, Mary

    2014-01-01

    Students with LD who struggle with reading comprehension can benefit from instruction on how to read strategically. One strategy that has been demonstrated to increase reading comprehension is self-questioning. In this study, two fifth graders with LD were taught to self-generate questions using a prompt fading procedure. The participants were…

  14. 75 FR 5071 - Mississippi L&D 21, LLC, Mississippi River No. 21 Hydropower Company Lock + TM

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-01

    ... Energy Regulatory Commission Mississippi L&D 21, LLC, Mississippi River No. 21 Hydropower Company Lock..., Mississippi L&D 21, LLC (Mississippi LLC) filed an application for a preliminary permit for the proposed Mississippi River Lock and Dam No. 21 Hydroelectric Project No. 13636. On December 2, 2009, Mississippi...

  15. Impact of the Personal Strengths Program on Self-Determination Levels of College Students with LD and/or ADHD

    ERIC Educational Resources Information Center

    Farmer, Jennie L.; Allsopp, David H.; Ferron, John M.

    2015-01-01

    This study investigates the impact of The Personal Strengths Program (PSP) on seven college students with learning disabilities and/or attention deficit hyperactivity disorder (LD/ADHD) using a multiple baseline design. Students with LD/ADHD experience increased challenges in school settings and decreased post-secondary outcomes when compared with…

  16. Helping Students with LD to Succeed: The Role of Teachers' Hope, Sense of Coherence and Specific Self-Efficacy

    ERIC Educational Resources Information Center

    Levi, Uzi; Einav, Michal; Raskind, Ilana; Ziv, Orit; Margalit, Malka

    2013-01-01

    Teachers play a critical role in facilitating the academic achievements of students with learning disabilities (LD). The personal resources of teachers, such as sense of coherence (SOC) and hopeful thinking, may predict self-perception of the competency and efficacy they possess to help students with LD acquire needed learning skills. Several…

  17. Personnel launch system (PLS) lifting body and low lift-to-drag (L/D)

    NASA Technical Reports Server (NTRS)

    Erwin, Harry O.

    1990-01-01

    The Personnel Launch System (PLS) is a small transportation system designed to transport people, but no cargo, to and from low-earth orbit. The PLS is being considered as an addition to the manned launch capability of the United States for three main reasons: (1) to assure manned access to space, (2) to achieve a first-stage abort ability, and (3) to reduce operations costs. To those ends, two designs are being considered for the PLS that differ in their lift-to-drag (L/D) ratio. The Lyndon B. Johnson Space Center was assigned the task of examining low L/D capsules with no wings and a parachute landing capability. The Langley Research Center is studying a higher L/D PLS with wings and runway landings. Whichever design is selected, the PLS will act as a complement to the Space Shuttle fleet and will enhance the ability of our Nation to achieve reliable, safe, and cost-effective access to space flight, thus furthering the goals of the U.S. space program and increasing the safety of the human crews manning a future space station.

  18. LD50 and repellent effects of essential oils from Argentinian wild plant species on Varroa destructor.

    PubMed

    Ruffinengo, Sergio; Eguaras, Martin; Floris, Ignazio; Faverin, Claudia; Bailac, Pedro; Ponzi, Marta

    2005-06-01

    The repellent and acaricidal effects of some essential oils from the most typical wild plant species of northern Patagonia, Argentina, on Varroa destructor Anderson & Trueman were evaluated using a complete exposure test. Honey bees, Apis mellifera L., and mites (five specimens of each per dish) were introduced in petri dishes having different oil concentrations (from 0.1 to 25 micro per cage). Survival of bees and mites was registered after 24, 48, and 72 h. An attraction/repellence test was performed using a wax tube impregnated with essential oil and another tube containing wax only. The lowest LD50 values for mites were registered for Acantholippia seriphioides (A. Gray) Mold. (1.27 microl per cage) and Schinus molle L. (2.65 microl per cage) after 24 h, and for Wedelia glauca (Ortega) O. Hoffm. ex Hicken (0.59 microl per cage) and A. seriphioides (1.09 microl per cage) after 72 h of treatment. The oil with the highest selectivity ratio (A. mellifera LD50/V. destructor LD50) was the one extracted from S. molle (>16). Oils of Lippia junelliana (Mold.) Troncoso, Minthostachys mollis (HBK) Grieseb., and Lippia turbinata Grieseb. mixed with wax had repellent properties. None of the oils tested had attractive effects on Varroa mites.

  19. Epoxy matrix composites filled with micro-sized LD sludge: wear characterization and analysis

    NASA Astrophysics Data System (ADS)

    Purohit, Abhilash; Satapathy, Alok

    2016-02-01

    Owing to the very high cost of conventional filler materials in polymer composites, exploring the possibility of using low cost minerals and industrial wastes for this purpose has become the need of the hour. In view of this, the present work includes the development and the wear performance evaluation of a new class of composites consisting of epoxy and microsized LD sludge. LD sludge or the Linz-Donawitz Sludge (LDS) are the fine solid particles recovered after wet cleaning of the gas emerging from LD convertors during steel making. Epoxy composites filled with different proportions (0, 5, 10, 15 and 20 wt %) of LDS are fabricated by conventional hand lay-up technique. Dry sliding wear trials are performed on the composite specimens under different test conditions as per ASTM G 99 following a design of experiment approach based on Taguchi's orthogonal arrays. The Taguchi approach leads to the recognition of most powerful variables that predominantly control the wear rate. This parametric analysis reveals that LDS content and sliding velocity affects the specific wear rate more significantly than normal load and sliding distance. Furthermore with increase in LDS content specific wear rate of the composite decreases for a constant sliding velocity. The sliding wear behavior of these composites under an extended range of test conditions is predicted by a model based on the artificial neural network (ANN).

  20. Thrombolytic effects of Douchi Fibrinolytic enzyme from Bacillus subtilis LD-8547 in vitro and in vivo

    PubMed Central

    2012-01-01

    Background Today, thrombosis is one of the most widely occurring diseases in modern life. Drugs with thrombolytic functions are the most effective methods in the treatment of thrombosis. Among them, Douchi fibrinolytic enzyme (DFE) is a promising agent. DFE was isolated from Douchi, a typical and popular soybean-fermented food in China, and it can dissolve fibrin directly and efficiently. A strain, Bacillus subtilis LD-8547 produced DFE with high fibrinolytic activity has been isolated in our lab previously. Results In the study, thrombolytic effect of DFE from Bacillus subtilis LD-8547 was studied in vitro and in vivo systematically. The results showed that DFE played a significant role in thrombolysis and anticoagulation in vitro. And the thrombolytic effects correlated with DFE in a dose-dependent manner. In vivo, the acute toxicity assay showed that DFE had no obvious acute toxicity to mice. Test of carrageenan-induced thrombosis in mice indicated that the DFE significantly prevented tail thrombosis, and arterial thrombosis model test indicated that Douchi fibrinolytic enzyme DFE had thrombolytic effect on carotid thrombosis of rabbits in vivo. Other results in vivo indicated that DFE could increase bleeding and clotting time obviously. Conclusions The DFE isolated from Bacillus subtilis LD-8547 has obvious thrombolytic effects in vitro and in vivo. This function demonstrates that this enzyme can be a useful tool for preventing and treating clinical thrombus. PMID:22748219

  1. An electrodynamics-Langevin dynamics (ED-LD) approach to simulate metal nanoparticle interactions and motion.

    PubMed

    Sule, N; Rice, S A; Gray, S K; Scherer, N F

    2015-11-16

    Understanding the formation of electrodynamically interacting assemblies of metal nanoparticles requires accurate computational methods for determining the forces and propagating trajectories. However, since computation of electromagnetic forces occurs on attosecond to femtosecond timescales, simulating the motion of colloidal nanoparticles on milliseconds to seconds timescales is a challenging multi-scale computational problem. Here, we present a computational technique for performing accurate simulations of laser-illuminated metal nanoparticles. In the simulation, we self-consistently combine the finite-difference time-domain method for electrodynamics (ED) with Langevin dynamics (LD) for the particle motions. We demonstrate the ED-LD method by calculating the 3D trajectories of a single 100-nm-diameter Ag nanoparticle and optical trapping and optical binding of two and three 150-nm-diameter Ag nanoparticles in simulated optical tweezers. We show that surface charge on the colloidal metal nanoparticles plays an important role in their optically driven self-organization. In fact, these simulations provide a more complete understanding of the assembly of different structures of two and three Ag nanoparticles that have been observed experimentally, demonstrating that the ED-LD method will be a very useful tool for understanding the self-organization of optical matter.

  2. How to find a leucine in a haystack? Structure, ligand recognition and regulation of leucine-aspartic acid (LD) motifs.

    PubMed

    Alam, Tanvir; Alazmi, Meshari; Gao, Xin; Arold, Stefan T

    2014-06-15

    LD motifs (leucine-aspartic acid motifs) are short helical protein-protein interaction motifs that have emerged as key players in connecting cell adhesion with cell motility and survival. LD motifs are required for embryogenesis, wound healing and the evolution of multicellularity. LD motifs also play roles in disease, such as in cancer metastasis or viral infection. First described in the paxillin family of scaffolding proteins, LD motifs and similar acidic LXXLL interaction motifs have been discovered in several other proteins, whereas 16 proteins have been reported to contain LDBDs (LD motif-binding domains). Collectively, structural and functional analyses have revealed a surprising multivalency in LD motif interactions and a wide diversity in LDBD architectures. In the present review, we summarize the molecular basis for function, regulation and selectivity of LD motif interactions that has emerged from more than a decade of research. This overview highlights the intricate multi-level regulation and the inherently noisy and heterogeneous nature of signalling through short protein-protein interaction motifs.

  3. 3D-MRI rendering of the anatomical structures related to acupuncture points of the Dai mai, Yin qiao mai and Yang qiao mai meridians within the context of the WOMED concept of lateral tension: implications for musculoskeletal disease

    PubMed Central

    Moncayo, Roy; Rudisch, Ansgar; Kremser, Christian; Moncayo, Helga

    2007-01-01

    Background A conceptual model of lateral muscular tension in patients presenting thyroid associated ophthalmopathy (TAO) has been recently described. Clinical improvement has been achieved by using acupuncture on points belonging to the so-called extraordinary meridians. The aim of this study was to characterize the anatomical structures related to these acupuncture points by means of 3D MRI image rendering relying on external markers. Methods The investigation was carried out the index case patient of the lateral tension model. A licensed medical acupuncture practitioner located the following acupuncture points: 1) Yin qiao mai meridian (medial ankle): Kidney 3, Kidney 6, the plantar Kidney 6 (Nan jing description); 2) Yang qiao mai meridian (lateral ankle): Bladder 62, Bladder 59, Bladder 61, and the plantar Bladder 62 (Nan jing description); 3) Dai mai meridian (wait): Liver 13, Gall bladder 26, Gall bladder 27, Gall bladder 28, and Gall bladder 29. The points were marked by taping a nitro-glycerin capsule on the skin. Imaging was done on a Siemens Magnetom Avanto MR scanner using an array head and body coil. Mainly T1-weighted imaging sequences, as routinely used for patient exams, were used to obtain multi-slice images. The image data were rendered in 3D modus using dedicated software (Leonardo, Siemens). Results Points of the Dai mai meridian – at the level of the waist – corresponded to the obliquus externus abdominis and the obliquus internus abdominis. Points of the Yin qiao mai meridian – at the medial side of the ankle – corresponded to tendinous structures of the flexor digitorum longus as well as to muscular structures of the abductor hallucis on the foot sole. Points of the Yang qiao mai meridian – at the lateral side of the ankle – corresponded to tendinous structures of the peroneus brevis, the peroneous longus, and the lateral surface of the calcaneus and close to the foot sole to the abductor digiti minimi. Conclusion This non

  4. High Density LD-Based Structural Variations Analysis in Cattle Genome

    PubMed Central

    Salomon-Torres, Ricardo; Matukumalli, Lakshmi K.; Van Tassell, Curtis P.; Villa-Angulo, Carlos; Gonzalez-Vizcarra, Víctor M.; Villa-Angulo, Rafael

    2014-01-01

    Genomic structural variations represent an important source of genetic variation in mammal genomes, thus, they are commonly related to phenotypic expressions. In this work, ∼770,000 single nucleotide polymorphism genotypes from 506 animals from 19 cattle breeds were analyzed. A simple LD-based structural variation was defined, and a genome-wide analysis was performed. After applying some quality control filters, for each breed and each chromosome we calculated the linkage disequilibrium (r2) of short range (≤100 Kb). We sorted SNP pairs by distance and obtained a set of LD means (called the expected means) using bins of 5 Kb. We identified 15,246 segments of at least 1 Kb, among the 19 breeds, consisting of sets of at least 3 adjacent SNPs so that, for each SNP, r2 within its neighbors in a 100 Kb range, to the right side of that SNP, were all bigger than, or all smaller than, the corresponding expected mean, and their P-value were significant after a Benjamini-Hochberg multiple testing correction. In addition, to account just for homogeneously distributed regions we considered only SNPs having at least 15 SNP neighbors within 100 Kb. We defined such segments as structural variations. By grouping all variations across all animals in the sample we defined 9,146 regions, involving a total of 53,137 SNPs; representing the 6.40% (160.98 Mb) from the bovine genome. The identified structural variations covered 3,109 genes. Clustering analysis showed the relatedness of breeds given the geographic region in which they are evolving. In summary, we present an analysis of structural variations based on the deviation of the expected short range LD between SNPs in the bovine genome. With an intuitive and simple definition based only on SNPs data it was possible to discern closeness of breeds due to grouping by geographic region in which they are evolving. PMID:25050984

  5. The GEOTAIL HEP-LD: The Work Of Berend Wilken Remembered

    NASA Astrophysics Data System (ADS)

    Zong, Q.

    Berend Wilken had published 32 refereed papers on Geotail project, we have co- operated 26 papers among them. In September 1992 the Japanese spacecraft GEOTAIL was launched to explore the nature of the magnetosphere's tail region (often referred to as magnetotail) over a large range in radial distance. Initially, the spacecraft was operated in a sequence of non-periodic orbits with apogees between 80 and 220 R_E in the magnetotail and perigees around 10 to 20 R_E at the day-side of the magnetosphere. Complex manoeuver in the gravitational fields of the Earth and the Moon made these kind of 'slingshot' orbits possible and kept apogee consistently in the tail region. In November 1994, GEOTAIL was directed to a standard elliptical 8 by 30 R_E orbit in which the satellite circulates Earth in the ecliptic plane. The scientific payload of GEOTAIL is composed of advanced instruments for the complete determination of electric and magnetic fields, waves, and plasma composition. The low energy particle spectrometer LD is part of the comprehensive HEP particle detector package. Energy range, mass resolution, and angular coverage makes the LD spectrometer ideal for studies of the energetic plasma component in geospace. The combined time-of-flight / energy tech- nique allows identification of ions between hydrogen and the Si-Fe group. The useful energy range extends up to 1500 keV for all species, however, the lower threshold is mass dependent (e.g. 50 keV for protons). The LD spectrometer returned a wealth of measurements both from the deep tail phase and from the near-Earth orbit. The main emphasis of this review is (a) on so-called plasmoids and their formation in the tail region and associated oxygen beams and (b) on particle beams of similar structure ob- served in the dayside magnetosheath during periods of magnetospheric disturbance. (c) Energetic particles behaviour in the tail during CIR/CME encounter the Earth.

  6. The Same Major Histocompatibility Complex Polymorphism Involved in Control of HIV Influences Peptide Binding in the Mouse H-2Ld System*

    PubMed Central

    Narayanan, Samanthi; Kranz, David M.

    2013-01-01

    Single-site polymorphisms in human class I major histocompatibility complex (MHC) products (HLA-B) have recently been shown to correlate with HIV disease progression or control. An identical single-site polymorphism (at residue 97) in the mouse class I product H-2Ld influences stability of the complex. To gain insight into the human polymorphisms, here we examined peptide binding, stability, and structures of the corresponding Ld polymorphisms, Trp97 and Arg97. Expression of LdW97 and LdR97 genes in a cell line that is antigen-processing competent showed that LdR97 was expressed at higher levels than LdW97, consistent with enhanced stability of self-peptide·LdR97 complexes. To further examine peptide-binding capacities of these two allelic variants, we used a high affinity pep-Ld specific probe to quantitatively examine a collection of self- and foreign peptides that bind to Ld. LdR97 bound more effectively than LdW97 to most peptides, although LdW97 bound more effectively to two peptides. The results support the view that many self-peptides in the Ld system (or the HLA-B system) would exhibit enhanced binding to Arg97 alleles compared with Trp97 alleles. Accordingly, the self-peptide·MHC-Arg97 complexes would influence T-cell selection behavior, impacting the T-cell repertoire of these individuals, and could also impact peripheral T cell activity through effects of self-peptide·Ld interacting with TCR and/or CD8. The structures of several peptide·LdR97 and peptide·LdW97 complexes provided a framework of how this single polymorphism could impact peptide binding. PMID:24064213

  7. English language learners with reading-related LD: linking data from multiple sources to make eligibility determinations.

    PubMed

    Wilkinson, Cheryl Y; Ortiz, Alba A; Robertson, Phyllis M; Kushner, Millicent I

    2006-01-01

    Results are reported for an exploratory study of eligibility decisions made for 21 Spanish-speaking English language learners (ELLs) with learning disabilities (LD) and no secondary disabilities who received special education support in reading. Eligibility determinations by an expert panel resulted in decisions that differed significantly from those of school multidisciplinary teams. The panel agreed that some students appeared to have reading-related LD (n = 5) but also identified students that they believed had disabilities, but not necessarily reading-related LD (n = 6). Another group of students (n = 10) had learning problems that the panel believed could be attributed to factors other than LD or for whom substantive additional data would be required to validate eligibility. Issues associated with referral, assessment, and eligibility determinations for ELLs are discussed, and recommendations for improving practice are offered, with an emphasis on the importance of linking data from multiple sources when deciding whether ELLs qualify for special education.

  8. Attention deficit and hyperactivity disorder (ADHD) and learning disabilities (LD): adolescents perspective.

    PubMed

    Brook, Uzi; Boaz, Mona

    2005-08-01

    Three hundred and eight pupils in the age group 12-18 years were interviewed and examined. They had been diagnosed as having attention deficit and hyperactivity disorder (ADHD) and learning disabilities (LD), and were attending a high school devoted to special education. Their classification into subgroups was as follows: ADHD - inattentive (I) = 22.1%, ADHD - hyperactivity/impulsivity (HI) = 12.3% and combined = 42.2%. Only 25% of them were treated by methylphenidate (Ritalin). Ninety-four percent of them were diagnosed with comorbidity of 'learning disabilities'. Thirty-four percent of them reported being severely stressed when going to school and sitting in class. Their complaints were: tiredness and excessive needs to sleep, frequent quarrelling with close friends, feeling different from other classmates and having low self-esteem (SE). They complaint that their parents don't understand them. Things that irritated them the most were being lied to and coercion by others. The authors suggest to consider ADHD/LD as neurobehavioral disability. It is mandatory to prepare them for adult life with proper social skills and a suitable occupation.

  9. Structure of the LdcB LD-carboxypeptidase reveals the molecular basis of peptidoglycan recognition.

    PubMed

    Hoyland, Christopher N; Aldridge, Christine; Cleverley, Robert M; Duchêne, Marie-Clémence; Minasov, George; Onopriyenko, Olena; Sidiq, Karzan; Stogios, Peter J; Anderson, Wayne F; Daniel, Richard A; Savchenko, Alexei; Vollmer, Waldemar; Lewis, Richard J

    2014-07-08

    Peptidoglycan surrounds the bacterial cytoplasmic membrane to protect the cell against osmolysis. The biosynthesis of peptidoglycan, made of glycan strands crosslinked by short peptides, is the target of antibiotics like β-lactams and glycopeptides. Nascent peptidoglycan contains pentapeptides that are trimmed by carboxypeptidases to tetra- and tripeptides. The well-characterized DD-carboxypeptidases hydrolyze the terminal D-alanine from the stem pentapeptide to produce a tetrapeptide. However, few LD-carboxypeptidases that produce tripeptides have been identified, and nothing is known about substrate specificity in these enzymes. We report biochemical properties and crystal structures of the LD-carboxypeptidases LdcB from Streptococcus pneumoniae, Bacillus anthracis, and Bacillus subtilis. The enzymes are active against bacterial cell wall tetrapeptides and adopt a zinc-carboxypeptidase fold characteristic of the LAS superfamily. We have also solved the structure of S. pneumoniae LdcB with a product mimic, elucidating the residues essential for peptidoglycan recognition and the conformational changes that occur on ligand binding.

  10. Study on the output of LD-pumped passively Q-switched subnanosecond microlasers

    NASA Astrophysics Data System (ADS)

    Cheng, He-jiao; Chen, Hui-min; Ding, Bo-sheng; Cai, De

    2013-09-01

    Caliber mortar projectiles and bombs are now tend to use the laser fuze because of its high accuracy in distance measurement,the strong ability to resist electromagnetic interference, the high angular resolution and the good concealment. Otherwise, the microlaser can bring about some alluring benefits. For instance, it can generate high quality laser beam, which has small divergence angle and high power. So, This paper summarizes some key factors which have impacts on the output of LD-pumped passively Q-switched subnanosecond microlasers, including the initial transmission of the absorber, the reflection of output coupler, the length of the cavity and the radius of the pumping laser beam, and gives the conclusion of the simulation based on the variation of the density of the reversal particle in the resonant cavity derived from the oscillation equation of the LD-pumped passively Q-switched Cr4+:YAG/ Nd:YAG laser, which provides the basis for the design of the passive Q-switched micro lasers which have high power, high repetition frequency and narrow pulse width.

  11. A Modular Artificial Intelligence Inference Engine System (MAIS) for support of on orbit experiments

    NASA Technical Reports Server (NTRS)

    Hancock, Thomas M., III

    1994-01-01

    This paper describes a Modular Artificial Intelligence Inference Engine System (MAIS) support tool that would provide health and status monitoring, cognitive replanning, analysis and support of on-orbit Space Station, Spacelab experiments and systems.

  12. In-vivo visualisation of the anatomical structures related to the acupuncture points Dai mai and Shen mai by MRI: A single-case pilot study

    PubMed Central

    Moncayo, Roy; Rudisch, Ansgar; Diemling, Markus; Kremser, Christian

    2007-01-01

    Background The concept of acupuncture point localisation in Traditional Chinese Medicine (TCM) is based on millenary practical experience. Modern imaging methods such as PET, MRI and SPECT have been used primary for the investigation of the mechanisms of action of acupuncture. In this pilot single-case study we have evaluated the technical possibilities for in-vivo imaging of the anatomical relations of acupuncture points using state of the art MRI. Methods Preliminary experiments relating to the quality of acupuncture needles under the setting of MRI were done both with stainless steel and gold needles. In a second step, in-vivo imaging was carried out. A licensed acupuncture practitioner (RM) chose two points belonging to the so-called extraordinary vessels. In 2 sequential, separate procedures, he inserted himself gold acupuncture needles using a neutral technique (known as Ping Bu Ping Xie) into the Dai mai and Shen mai points, i.e. gall bladder 26 and bladder 62. Imaging was done on a Siemens Magnetom Avanto MR scanner using a head array and body coil. Mainly T1-weighted imaging sequences, as routinely used for patient exams, were used to obtain multi-slice images. Results In the preliminary experiments only acupuncture needles made of gold showed enough stability in order to be used for further imaging procedures. Using an onion and a banana as an object, further studies showed that the gold needles produced a void defect that corresponds to the tip of the inserted needle, while at the same time an artefactually increased diameter was observed. The in-vivo experiments showed that the Dai mai point was in relation to the abdominal internal oblique muscle. The Shen mai point artefact showed up close to the longus and brevis peroneal tendons at the fibular malleolus. Side effects related to heating or burning were not observed. Improved anatomical recognition was obtained using 3D-volume rendering techniques. Conclusion Through an adequate choice of acupuncture

  13. Theoretical Accuracy of Along-Track Displacement Measurements from Multiple-Aperture Interferometry (MAI)

    PubMed Central

    Jung, Hyung-Sup; Lee, Won-Jin; Zhang, Lei

    2014-01-01

    The measurement of precise along-track displacements has been made with the multiple-aperture interferometry (MAI). The empirical accuracies of the MAI measurements are about 6.3 and 3.57 cm for ERS and ALOS data, respectively. However, the estimated empirical accuracies cannot be generalized to any interferometric pair because they largely depend on the processing parameters and coherence of the used SAR data. A theoretical formula is given to calculate an expected MAI measurement accuracy according to the system and processing parameters and interferometric coherence. In this paper, we have investigated the expected MAI measurement accuracy on the basis of the theoretical formula for the existing X-, C- and L-band satellite SAR systems. The similarity between the expected and empirical MAI measurement accuracies has been tested as well. The expected accuracies of about 2–3 cm and 3–4 cm (γ = 0.8) are calculated for the X- and L-band SAR systems, respectively. For the C-band systems, the expected accuracy of Radarsat-2 ultra-fine is about 3–4 cm and that of Sentinel-1 IW is about 27 cm (γ = 0.8). The results indicate that the expected MAI measurement accuracy of a given interferometric pair can be easily calculated by using the theoretical formula. PMID:25251408

  14. Polymorphs of Aspirin Solid-state IR-LD spectroscopic and quantitative determination in solid mixtures

    NASA Astrophysics Data System (ADS)

    Koleva, Bojidarka B.

    2006-12-01

    Solid-state linear-dichroic infrared (IR-LD) spectroscopy, using an orientation technique as a suspension in nematic liquid crystal, has been carried out of Aspirin polymorphs (forms I and II). Reducing-difference procedure for polarized IR-spectra interpretation has been applied for structural analysis of both modifications and the data have been compared with known crystallographic ones. A vibration assignment of forms I and II has been included and on this basis, a quantitative determination by FT-IR spectra for form I in mixtures with second one has been presented, using intensity ratio of 1606 cm -1 peak (characteristic for both forms) to 599 cm -1 one (attributed to form I). The obtained reliability is 99.78%.

  15. LD-cladding-pumped 50 pm linewidth Tm 3+ -doped silica fiber laser.

    PubMed

    Yunjun, Zhang; Baoquan, Yao; Youlun, Ju; Hui, Zhou; Yuezhu, Wang

    2008-05-26

    We report on a Tm(3+)-doped fiber laser source operating at 1936.4 nm with a very narrow linewidth (50 pm) laser output. Up to 2.4 W cw laser power was obtained from an 82 cm long Tm(3+)-doped multimode-core fiber cladding pumped by a 792 nm laser diode (LD). The fiber laser cavity included a high-reflective dichroic and a low-reflective FBG output coupler. The multimode fiber Bragg grating (FBG) transmission spectrum and output laser spectrum were measured. By adjusting the distance between the dichroic and the Tm(3+)-doped fiber end, the multipeak laser spectrum changed to a single-peak laser spectrum.

  16. LD bar corner-pumped TEM00 CW composite Nd:YAG laser.

    PubMed

    Gao, Song; Liu, Huan; Wang, Dongsheng; Gong, Mali

    2009-11-23

    Ray-tracing algorithm was used to simulate the pump absorption efficiency and pump absorption distribution of LD corner-pumped laser with different parameters of Nd:YAG composite slab crystal. Resonator experiment was performed to suppress the oscillation of higher-order modes and realize TEM(00) operation. Experiment results showed that the output power was 11.94 W with an optical-optical efficiency of over 26%, and the M2 factors of beam quality at width and thickness directions were 1.18 and 1.34, respectively. It has been proven that the corner-pumped configuration could obtain laser output with good beam quality as a result of high pump efficiency and good pump uniformity.

  17. Two phase choke flow in tubes with very large L/D

    NASA Technical Reports Server (NTRS)

    Hendricks, R. C.; Simoneau, R. J.

    1977-01-01

    Data were obtained for two phase and gaseous choked flow nitrogen in a long constant area duct of 16200 L/D with a diverging diffuser attached to the exit. Flow rate data were taken along five isotherms (reduced temperature of 0.81, 0.96, 1.06, 1.12, and 2.34) for reduced pressures to 3. The flow rate data were mapped in the usual manner using stagnation conditions at the inlet mixing chamber upstream of the entrance length. The results are predictable by a two phase homogeneous equilibrium choking flow model which includes wall friction. A simplified theory which in essence decouples the long tube region from the high acceleration choking region also appears to predict the data resonably well, but about 15 percent low.

  18. How do Data Processing Choices Affect Multiple Aperture InSAR (MAI) Precision?

    NASA Astrophysics Data System (ADS)

    Bechor-Ben Dov, N.

    2015-12-01

    MAI data processing methods report different precisions, sometimes for the same data; we investigate these discrepancies here. MAI, a method to obtain phase-based along-track displacements from InSAR data, has been used for measuring volcanic, glacier, and earthquake displacements. However, different applications show vastly different precisions, ranging from a few millimeters to tens of centimeters per interferogram. While some spatial variability in precision is expected due to the InSAR phase noise dependence on terrain, some reports show different precisions for the same data. Here, I address the following questions: What is the true precision of MAI measurements? What are the MAI noise sources, and how do we mitigate them? We use data from 11 Envisat, 2 ERS, and 1 ALOS interferograms to analyze and quantify the MAI noise sources and their dependence on the method's implementation. To do that, we implement various processing steps with the JPL/Caltech InSAR data processor ROI_PAC. We find that there are several (sometimes surprising) nuances in data processing that together can reduce scatter noise by half, and other corrections that can reduce topography-related noise by up to 25 centimeters. We find that even with all the optimized data processing steps, the actual MAI errors (relative accuracy) are higher then the theoretical ones by a factor that depends on filter window size. We present an updated MAI data processor, which is capable of achieving relative accuracy and precision levels of 6 to 2 centimeters, respectively, in low to medium coherence terrains (correlation coefficient of 0.4 to 0.8), and provides error estimates that better reflect the actual relative accuracy of the measurement.

  19. Oral LD50 toxicity modeling and prediction of per- and polyfluorinated chemicals on rat and mouse.

    PubMed

    Bhhatarai, Barun; Gramatica, Paola

    2011-05-01

    Quantitative structure-activity relationship (QSAR) analyses were performed using the LD(50) oral toxicity data of per- and polyfluorinated chemicals (PFCs) on rodents: rat and mouse. PFCs are studied under the EU project CADASTER which uses the available experimental data for prediction and prioritization of toxic chemicals for risk assessment by using the in silico tools. The methodology presented here applies chemometrical analysis on the existing experimental data and predicts the toxicity of new compounds. QSAR analyses were performed on the available 58 mouse and 50 rat LD(50) oral data using multiple linear regression (MLR) based on theoretical molecular descriptors selected by genetic algorithm (GA). Training and prediction sets were prepared a priori from available experimental datasets in terms of structure and response. These sets were used to derive statistically robust and predictive (both internally and externally) models. The structural applicability domain (AD) of the models were verified on 376 per- and polyfluorinated chemicals including those in REACH preregistration list. The rat and mouse endpoints were predicted by each model for the studied compounds, and finally 30 compounds, all perfluorinated, were prioritized as most important for experimental toxicity analysis under the project. In addition, cumulative study on compounds within the AD of all four models, including two earlier published models on LC(50) rodent analysis was studied and the cumulative toxicity trend was observed using principal component analysis (PCA). The similarities and the differences observed in terms of descriptors and chemical/mechanistic meaning encoded by descriptors to prioritize the most toxic compounds are highlighted.

  20. Social interaction and sex differences influence rat temperature circadian rhythm under LD cycles and constant light.

    PubMed

    Cambras, T; Castejón, L; Díez-Noguera, A

    2011-06-01

    Circadian rhythms produce an efficient organization of animal behaviour over the 24h day. In some species, social cues have been found to have a role as synchronizers of these rhythms. Here, the influence of social interaction on rat circadian behaviour was investigated, addressing the question of whether cohabitation would produce a delay in the appearance of arrhythmicity under constant light conditions. To this end, the circadian rhythms of male and female rat body temperature were studied for 10days under light-dark conditions, followed by 33days under constant bright light. Half of the animals were maintained in individual cages, whilst the others were maintained in larger cages in groups of three rats of the same sex. Results showed that individual circadian rhythms under 24hour light-dark (LD) cycles were more stable and with higher amplitude in grouped than in isolated animals, and higher in males than in females. During the first days under constant light (LL), the stability of the rhythm was also higher in males than in females, but there were no differences according to the group. Moreover, we did not find significant differences in the time of circadian rhythm loss under LL, since high individual variability was found for this variable. On the other hand, female rats living in isolation showed a delayed acrophase in the circadian rhythm under LD conditions compared with those living in groups. These results suggest that cohabitation increases the internal coherence of circadian behaviour, and could be interpreted as indicating that living in isolation may induce a level of stress that disturbs manifestation of the circadian rhythm, especially in females, which are also more reactive than males to external signals.

  1. Validation of three adaptations of the Meaningful Auditory Integration Scale (MAIS) to German, English and Polish.

    PubMed

    Weichbold, Viktor; Anderson, Ilona; D'Haese, Patrick

    2004-03-01

    The Meaningful Auditory Integration Scale (MAIS) is a parent-report questionnaire for assessing auditory behaviour in aurally habilitated children. This study addressed the reliability and convergent validity of three different language versions of the MAIS: English, German, and Polish. In total, 114 parents (English, n = 27: Polish, n = 37; German, n = 50) completed the MAIS preoperatively and at 6 months after cochlear implantation. Internal reliability (Cronbach's alpha) ranged from 0.92 to 0.95 preoperatively, and from 0.87 to 0.93 at 6 months. Split-half reliability was at least 0.90 preoperatively, and ranged from 0.76 to 0.89 at 6 months. Corrected item-total correlation coefficients were significant (p < 0.05) for all items except for item 1, which showed poor correlations in the Polish version. Correlation of the MAIS with the Listening Progress Profile (LP), as a measure for convergent validity, yielded coefficients between 0.81 and 0.73 preoperatively, and between 0.79 and 0.61 at 6 months. These findings demonstrate high reliability and convergent validity of the three MAIS versions.

  2. Computer Supported Indexing: A History and Evaluation of NASA's MAI System

    NASA Technical Reports Server (NTRS)

    Silvester, June P.

    1997-01-01

    Computer supported or machine aided indexing (MAI) can be categorized in multiple ways. The system used by the National Aeronautics and Space Administration's (NASA's) Center for AeroSpace Information (CASI) is described as semantic and computational. It's based on the co-occurrence of domain-specific terminology in parts of a sentence, and the probability that an indexer will assign a particular index term when a given word or phrase is encountered in text. The NASA CASI system is run on demand by the indexer and responds in 3 to 9 seconds with a list of suggested, authorized terms. The system was originally based on a syntactic system used in the late 1970's by the Defense Technical Information Center (DTIC). The NASA mainframe-supported system consists of three components: two programs and a knowledge base (KB). The evolution of the system is described and flow charts illustrate the MAI procedures. Tests used to evaluate NASA's MAI system were limited to those that would not slow production. A very early test indicated that MAI saved about 3 minutes and provided several additional terms for each document indexed. It also was determined that time and other resources spent in careful construction of the KB pay off with high-quality output and indexer acceptance of MAI results.

  3. The subunit gene Ldα1 of nicotinic acetylcholine receptors plays important roles in the toxicity of imidacloprid and thiamethoxam against Leptinotarsa decemlineata.

    PubMed

    Qu, Yang; Chen, Jinhua; Li, Chenge; Wang, Qiang; Guo, Wenchao; Han, Zhaojun; Jiang, Weihua

    2016-02-01

    Nicotinic acetylcholine receptors (nAChRs) are pentameric ACh-gated ion channels. It is believed that nAChRs composed of different subunits may vary in their function and toxicological characteristics. Neonicotinoids are activators of nAChRs and important insecticides that are extensively used for crop protection and resistance has been developed by some pests. They are also major insecticides for the control of Leptinotarsa decemlineata, which is a destructive defoliator pest that invaded the Xinjiang region of China in the 1990s. However, little is known about the constitution or subunits of the target in this pest. In this study, the full-length cDNAs encoding four new nAChR subunits (named Ldα3, Ldα6, Ldα10, and Ldβ1) were cloned from L. decemlineata. These genes encode 822-, 753-, 672-, and 759-amino acid proteins, respectively, which share typical features of insect nAChRs subunits and closely resemble the corresponding subunits of the nAChRs from Tribolium castaneum. Temporal and spatial expression analyses showed that these genes, as well as the previously identified Ldα1, Ldα2, and Ldα8 genes, are widely expressed in all developmental stages, including eggs, larvae of various instars, pupae, and adults. All genes monitored were expressed at higher levels in the head than in the thorax and abdomen, except for Ldα10. Dietary ingestion of double-stranded RNA bacterially expressed for Ldα1 (dsLdα1) significantly reduced the mRNA level of Ldα1 in treated larvae and adults by 48.0% and 78.6%, respectively. Among the non-target genes, Ldα3, Ldα9, and Ldβ1 were significantly up-regulated in larvae. A toxicity bioassay showed that dsLdα1 treatment greatly decreased the sensitivity to imidacloprid and thiamethoxam in adults. The larval susceptibility to thiamethoxam but not to imidacloprid was also reduced because of the lower down-regulation of Ldα1. Thus, our results suggest that Ldα1 encodes a subunit of a functional nAChR that mediates the

  4. Screening for specific learning difficulties (SpLD): the impact upon the progression of pre-registration nursing students.

    PubMed

    Wray, Jane; Aspland, Jo; Taghzouit, Jayne; Pace, Kerry; Harrison, Paula

    2012-01-01

    The study aimed to explore the impact of screening pre-registration nursing students for an SpLD at entry to an education programme, and the provision of specialist support for those identified as 'at risk'. Progression was monitored using a multiple method approach. 242 (98%) students completed and returned the Adult Dyslexia Check List (ADCL). 69 scored 7 or above (28.5% of responses) and 36 (52%) went on for further assessment. 48% of students who scored 7+ did not go on to contact Disability Services at all. 11% of the cohort was formally identified as having a SpLD at the end of the year compared to 3.9% of students with a declared disability entering pre-registration nurse education in 2007. 54% of the students who scored 7+ successfully progressed into year two, compared with 41% of students with SpLD from a previous cohort.

  5. An optical pulse width modulation generator based on the injection-locking property of single mode FP-LD

    NASA Astrophysics Data System (ADS)

    Tran, Quoc Hoai; Nakarmi, Bikash; Won, Yong Hyub

    2013-03-01

    A novel simple optical pulse width modulation generator (OPWMG) based on injection-locking property of a single mode FP-LD (SMFP-LD) has been proposed and experimentally verified. The OPWMG consists of a SMFP-LD (which acts as comparator), an optical sinusoidal wave source (analog input), and a continuous optical beam (control signal). The power required for fully injection-locking the SMFP-LD acts as the referent power whereas the combination power of continuous optical beam and analog optical sinusoidal signals work as control signals for changing the duty cycle of the proposed OPWMG. The presence of only continuous optical beam is not sufficient to suppress the dominant mode of SMFP-LD with high ON/OFF contrast ratio; however, the application of additional sinusoidal wave of constant amplitude and frequency, the dominant mode of SMFP-LD can be suppressed for the certain time window. Since, injection-locking power is dependent with the combined power of input injected continuous beam and sinusoidal optical wave, the time window of injection-locking can be varied by changing input beam power which provides different duty cycle of 13% to 68% at the output. Current available schemes for generating PWM signals are in electrical domain, hence, they need to convert electrical signals into optical domain by using expensive O/E converters for application in optical control and signal processing. The proposed OPWMG scheme has several advantages, such as low cost, low power consumption (~0.5 mW) which can be used for various applications where the effect of EMI/EMR is considered as an important factor such as control circuit for high voltage converters in power plant and electrical vehicles.

  6. The LD4 motif of paxillin regulates cell spreading and motility through an interaction with paxillin kinase linker (PKL).

    PubMed

    West, K A; Zhang, H; Brown, M C; Nikolopoulos, S N; Riedy, M C; Horwitz, A F; Turner, C E

    2001-07-09

    The small GTPases of the Rho family are intimately involved in integrin-mediated changes in the actin cytoskeleton that accompany cell spreading and motility. The exact means by which the Rho family members elicit these changes is unclear. Here, we demonstrate that the interaction of paxillin via its LD4 motif with the putative ARF-GAP paxillin kinase linker (PKL) (Turner et al., 1999), is critically involved in the regulation of Rac-dependent changes in the actin cytoskeleton that accompany cell spreading and motility. Overexpression of a paxillin LD4 deletion mutant (paxillinDeltaLD4) in CHO.K1 fibroblasts caused the generation of multiple broad lamellipodia. These morphological changes were accompanied by an increase in cell protrusiveness and random motility, which correlated with prolonged activation of Rac. In contrast, directional motility was inhibited. These alterations in morphology and motility were dependent on a paxillin-PKL interaction. In cells overexpressing paxillinDeltaLD4 mutants, PKL localization to focal contacts was disrupted, whereas that of focal adhesion kinase (FAK) and vinculin was not. In addition, FAK activity during spreading was not compromised by deletion of the paxillin LD4 motif. Furthermore, overexpression of PKL mutants lacking the paxillin-binding site (PKLDeltaPBS2) induced phenotypic changes reminiscent of paxillinDeltaLD4 mutant cells. These data suggest that the paxillin association with PKL is essential for normal integrin-mediated cell spreading, and locomotion and that this interaction is necessary for the regulation of Rac activity during these events.

  7. Graphic organizers and their effects on the reading comprehension of students with LD: a synthesis of research.

    PubMed

    Kim, Ae-Hwa; Vaughn, Sharon; Wanzek, Jeanne; Wei, Shangjin

    2004-01-01

    Previous research studies examining the effects of graphic organizers on reading comprehension for students with learning disabilities (LD) are reviewed. An extensive search of the professional literature between 1963 and June 2001 yielded a total of 21 group design intervention studies that met the criteria for inclusion in the synthesis. Using graphic organizers (i.e., semantic organizers, framed outlines, cognitive maps with and without a mnemonic) was associated with improved reading comprehension overall for students with LD. Compared to standardized reading measures, researcher-developed comprehension measures were associated with higher effect sizes. Initial gains demonstrated when using graphic organizers were not revealed during later comprehension tasks or on new comprehension tasks.

  8. Differentiating Literacy Growth of ELL Students with LD from Other High-Risk Subgroups and General Education Peers: Evidence from Grades 3-10

    ERIC Educational Resources Information Center

    Solari, Emily J.; Petscher, Yaacov; Folsom, Jessica Sidler

    2014-01-01

    The authors used a large data set (N = 1,011,549) to examine literacy growth over a single school year comparing general education (GenEd) students to three high-risk subgroups: English language learners (ELL), those with a specific learning disability (LD), and those identified as both LD and ELL (LD-ELL) in students in Grades 3-10. The authors…

  9. Studying Disability and Disability Studies: Shifting Paradigms of LD -- A Synthesis of Responses to Reid and Valle

    ERIC Educational Resources Information Center

    Connor, David J.

    2005-01-01

    In this article, I discuss the 11 diverse responses to Reid and Valle's work on the discursive practice of learning disabilities (LD), implications for instruction, and parent-school relations. I highlight key ideas from each article and then focus on three common areas of interest shared by most respondents: the unacceptable status quo of…

  10. How Should Middle-School Students with LD Approach Online Note Taking? A Mixed-Methods Study

    ERIC Educational Resources Information Center

    Igo, L. Brent; Riccomini, Paul J.; Bruning, Roger H.; Pope, Ginger G.

    2006-01-01

    This explanatory sequential mixed-methods study explored how the encoding of text ideas is affected when students with learning disabilities (LD) take notes from Web-based text. In the quantitative phase of the study, 15 students took three kinds of notes--typed, copy and paste, and written--with each kind of notes addressing a different topic.…

  11. Helping Students with Disabilities Transition to College: 21 Tips for Students with LD and/or ADD/ADHD

    ERIC Educational Resources Information Center

    Connor, David J.

    2012-01-01

    Making the transition from high school to college poses challenges for most students. Moving from a secure, regulated world of secondary education into an unfamiliar environment requiring greater independence can be a destabilizing experience. For students with learning disabilities (LD) and/or Attention Deficit Disorder (ADD), managing this…

  12. Probiotic Potential of Lactobacillus plantarum LD1 Isolated from Batter of Dosa, a South Indian Fermented Food.

    PubMed

    Gupta, Aabha; Tiwari, Santosh Kumar

    2014-06-01

    Lactobacillus plantarum LD1 was isolated from dosa batter and identified by biochemical, physiological and genetic methods. Species level identification was done by 16S rDNA amplification and sequencing. The probiotic potential of strain LD1 was assessed by different standard parameters. Cell surface hydrophobicity was recorded to be 62% with SAT value <0.007 M. Seventy-eight percent of viable count was found after treatment with simulated gastric juice containing pepsin (pH 2.0). Bile salt tolerance and bile salt hydrolase activity were also demonstrated by strain LD1. The culture supernatant was able to inhibit food-borne as well as clinical pathogenic microorganisms such as Staphylococcus aureus, Salmonella typhimurium, Shigella flexneri, Pseudomonas aeruginosa, urogenic Escherichia coli and Vibrio sp. Strain LD1 was found to be sensitive to most of the antibiotics used in the study. Since strain has been isolated from food source that is most typical of Southern India, it would be safe for further consumption in probiotic products.

  13. A Synthesis of Spelling and Reading Interventions and Their Effects on the Spelling Outcomes of Students with LD

    ERIC Educational Resources Information Center

    Wanzek, Jeanne; Vaughn, Sharon; Wexler, Jade; Swanson, Elizabeth A.; Edmonds, Meghan; Kim, Ae-Hwa

    2006-01-01

    Previous research studies examining the effects of spelling and reading interventions on the spelling outcomes of students with learning disabilities (LD) are synthesized. An extensive search of the professional literature between 1995 and 2003 yielded a total of 19 intervention studies that provided spelling and reading interventions to students…

  14. Some aspects of a free jet phenomena to 105 L/D in a constant area duct

    NASA Technical Reports Server (NTRS)

    Hendricks, R. C.

    1979-01-01

    Under certain conditions, inlets with a Borda type geometry were shown to exhibit sufficiently strong separation effects to permit the working fluid to flow through the duct as if it were a free jet. Mass limiting flow data and associated pressure profiles for tubes of 14, 53, 64, 73, and 105 L/D with a Borda type inlet were taken to determine bounds of the free jet phenomena. For a given tube roughness, the limits appear to be one dimensional and dependent only on inlet stagnation conditions. For smooth tubes the upper L/D boundary is related by P sub R roughly equal to CT to the 17th power and sub R, C roughly equal to 00017 (L/D) to the 2.5 power where F sub R = P/P sub c is reduced pressure and T sub R = T/T sub c is reduced temperature. The lower bound appears to be saturation conditions at the inlet. Similar free jet effects were found for fluid hydrogen indicating that fluid jetting may be common to all fluids. While limited data on surface roughness show a decrease in the upper L/D limit, nevertheless fluid jetting still occurred.

  15. Recombinant Leishmania Rab6 (rLdRab6) is recognized by sera from visceral leishmaniasis patients.

    PubMed

    Chauhan, Indira Singh; Shukla, Rantidev; Krishna, Shagun; Sekhri, Savita; Kaushik, Umesh; Baby, Sabitha; Pal, Chiranjib; Siddiqi, Mohammad Imran; Sundar, Shyam; Singh, Neeloo

    2016-11-01

    Rab proteins form the largest branch of the Ras superfamily. Rab proteins are key regulators of intracellular vesicular transport and membrane trafficking. Although RabGTPases are well-recognized targets in human diseases but are under-explored therapeutically in the Leishmania parasite. Using a quantitative cytofluorimetric assay, we analyzed the composition and organization of Rab6GTPase protein which was found to be primarily localized on the parasite subpellicular membrane and flagellum due to its association with kinesin motor proteins in the cytoskeletal microtubules. Our aim was to also assess the diagnostic role of recombinant Rab6 protein from Leishmania donovani (rLdRab6) using sera/plasma of Indian visceral leishmaniasis (VL) patients. Receiver-operating characteristic (ROC) curve analysis indicated 100% sensitivity and 100% specificity for rLdRab6-based ELISA which was almost similar in comparison to recombinant K39-based ELISA (95.83% sensitivity and 100% specificity). Sera of patients from another intracellular pathogenic infection, Mycobacterium tuberculosis, did not contain any significant levels of anti-rLdRab6 antibody. Thus rLdRab6 accuracy in visceral leishmaniasis diagnosis makes it a promising antigen for clinical use.

  16. Virtual Connections, Personal Resources, Loneliness, and Academic Self-Efficacy among College Students with and without LD

    ERIC Educational Resources Information Center

    Sharabi, Adi; Sade, Sarit; Margalit, Malka

    2016-01-01

    The goals of the study were first to compare the social and academic well-being (loneliness and academic self-efficacy (ASE) among college students with and without learning disabilities (LD), as well as three personal strengths (hope, optimism and sense of coherence (SOC). The second goal was to identify the predicting factors to their loneliness…

  17. Teaching Business Law to Non-Law Students, Culturally and Linguistically Diverse ("CaLD") Students, and Large Classes

    ERIC Educational Resources Information Center

    Kariyawasam, Kanchana; Low, Hang Yen

    2014-01-01

    This paper is largely based on the experience of teaching law to students with non-legal background in business schools, with a focus on internationalisation and the large class lecture format. Business schools often consist of large classes which include a significant proportion of Culturally and Linguistically Diverse (CaLD) students. Teaching a…

  18. Before the Well Runs Dry: Taking Care of Yourself as the Parent of a LD/ADD Child.

    ERIC Educational Resources Information Center

    Gehret, Jeanne

    Addressed to parents of children with learning disabilities (LD) or attention deficit disorder, this paper explores the dangers of falling into codependent behaviors and stresses the importance of parents taking care of themselves. The codependent parent is described as always putting the child first and as trying to control everything about the…

  19. Structure and Function of a Novel ld-Carboxypeptidase A Involved in Peptidoglycan Recycling

    PubMed Central

    Das, Debanu; Hervé, Mireille; Elsliger, Marc-André; Kadam, Rameshwar U.; Grant, Joanna C.; Chiu, Hsiu-Ju; Knuth, Mark W.; Klock, Heath E.; Miller, Mitchell D.; Godzik, Adam; Lesley, Scott A.; Deacon, Ashley M.

    2013-01-01

    Approximately 50% of cell wall peptidoglycan in Gram-negative bacteria is recycled with each generation. The primary substrates used for peptidoglycan biosynthesis and recycling in the cytoplasm are GlcNAc-MurNAc(anhydro)-tetrapeptide and its degradation product, the free tetrapeptide. This complex process involves ∼15 proteins, among which the cytoplasmic enzyme ld-carboxypeptidase A (LdcA) catabolizes the bond between the last two l- and d-amino acid residues in the tetrapeptide to form the tripeptide, which is then utilized as a substrate by murein peptide ligase (Mpl). LdcA has been proposed as an antibacterial target. The crystal structure of Novosphingobium aromaticivorans DSM 12444 LdcA (NaLdcA) was determined at 1.89-Å resolution. The enzyme was biochemically characterized and its interactions with the substrate modeled, identifying residues potentially involved in substrate binding. Unaccounted electron density at the dimer interface in the crystal suggested a potential site for disrupting protein-protein interactions should a dimer be required to perform its function in bacteria. Our analysis extends the identification of functional residues to several other homologs, which include enzymes from bacteria that are involved in hydrocarbon degradation and destruction of coral reefs. The NaLdcA crystal structure provides an alternate system for investigating the structure-function relationships of LdcA and increases the structural coverage of the protagonists in bacterial cell wall recycling. PMID:24123814

  20. LD-SPatt: large deviations statistics for patterns on Markov chains.

    PubMed

    Nuel, G

    2004-01-01

    Statistics on Markov chains are widely used for the study of patterns in biological sequences. Statistics on these models can be done through several approaches. Central limit theorem (CLT) producing Gaussian approximations are one of the most popular ones. Unfortunately, in order to find a pattern of interest, these methods have to deal with tail distribution events where CLT is especially bad. In this paper, we propose a new approach based on the large deviations theory to assess pattern statistics. We first recall theoretical results for empiric mean (level 1) as well as empiric distribution (level 2) large deviations on Markov chains. Then, we present the applications of these results focusing on numerical issues. LD-SPatt is the name of GPL software implementing these algorithms. We compare this approach to several existing ones in terms of complexity and reliability and show that the large deviations are more reliable than the Gaussian approximations in absolute values as well as in terms of ranking and are at least as reliable as compound Poisson approximations. We then finally discuss some further possible improvements and applications of this new method.

  1. A low timing jitter picosecond microchip laser pumped by pulsed LD

    NASA Astrophysics Data System (ADS)

    Wang, Sha; Wang, Yan-biao; Feng, Guoying; Zhou, Shou-huan

    2016-07-01

    SESAM passively Q-switched microchip laser is a very promising instrument to replace mode locked lasers to obtain picosecond pulses. The biggest drawback of a passively Q-switched microchip laser is its un-avoided large timing jitter, especially when the pump intensity is low, i.e. at low laser repetition rate range. In order to obtain a low timing jitter passively Q-switched picosecond microchip laser in the whole laser repetition rate range, a 1000 kHz pulsed narrow bandwidth Fiber Bragg Grating (FBG) stablized laser diode was used as the pump source. By tuning the pump intensity, we could control the output laser frequency. In this way, we achieved a very low timing jitter passively Q-switched picosecond laser at 2.13 mW, 111.1 kHz. The relative timing jitter was only 0.0315%, which was around 100 times smaller compared with a cw LD pumped microchip working at hundred kilohertz repetition rate frequency range.

  2. Structure and function of a novel LD-carboxypeptidase a involved in peptidoglycan recycling.

    PubMed

    Das, Debanu; Hervé, Mireille; Elsliger, Marc-André; Kadam, Rameshwar U; Grant, Joanna C; Chiu, Hsiu-Ju; Knuth, Mark W; Klock, Heath E; Miller, Mitchell D; Godzik, Adam; Lesley, Scott A; Deacon, Ashley M; Mengin-Lecreulx, Dominique; Wilson, Ian A

    2013-12-01

    Approximately 50% of cell wall peptidoglycan in Gram-negative bacteria is recycled with each generation. The primary substrates used for peptidoglycan biosynthesis and recycling in the cytoplasm are GlcNAc-MurNAc(anhydro)-tetrapeptide and its degradation product, the free tetrapeptide. This complex process involves ∼15 proteins, among which the cytoplasmic enzyme ld-carboxypeptidase A (LdcA) catabolizes the bond between the last two l- and d-amino acid residues in the tetrapeptide to form the tripeptide, which is then utilized as a substrate by murein peptide ligase (Mpl). LdcA has been proposed as an antibacterial target. The crystal structure of Novosphingobium aromaticivorans DSM 12444 LdcA (NaLdcA) was determined at 1.89-Å resolution. The enzyme was biochemically characterized and its interactions with the substrate modeled, identifying residues potentially involved in substrate binding. Unaccounted electron density at the dimer interface in the crystal suggested a potential site for disrupting protein-protein interactions should a dimer be required to perform its function in bacteria. Our analysis extends the identification of functional residues to several other homologs, which include enzymes from bacteria that are involved in hydrocarbon degradation and destruction of coral reefs. The NaLdcA crystal structure provides an alternate system for investigating the structure-function relationships of LdcA and increases the structural coverage of the protagonists in bacterial cell wall recycling.

  3. Welding Induced Alignment Distortion in Dual-in-Line LD Packages

    SciTech Connect

    Liu, Wenning N.; Sun, Xin; Khaleel, Mohammad A.; Shi, Frank G.

    2007-11-11

    The tolerance for the movement of a single mode fiber relative to the laser is extremely tight, a submicron movement can often lead to a significant misalignment and thus the reduction in the power coupled into the fiber. Among various fiber pigtailing assembly technologies, pulsed laser welding is the method with submicron accuracy and is most conducive to automation. However, the melting-solidification process during laser welding can often distort the pre-achieved fiber-optic alignment. This Welding-Induced-Alignment-Distortion (WIAD) is a serious concern and significantly affects the yield for single mode fiber pigtailing to a semiconductor laser. In this paper, effect of laser welding sequence on WIAD in a dual-in-line packager is numerically investigated by means of Finite Element Method (FEM). Optimal welding sequence may minimize WIAD in dual-in-line package. Additionally, unsymmetrical space between fiber and U-channel induced by laser welding of U-channel–to-plate in DIP LD packages is found to have obvious effect on WIAD.

  4. Measurements in film cooling flows: Hole L/D and turbulence intensity effects

    SciTech Connect

    Burd, S.W.; Kaszeta, R.W.; Simon, T.W.

    1996-12-31

    Hot-wire anemometry of simulated film cooling was used to study the influence of freestream turbulence intensity and film cooling hole length-to-diameter ratio on mean velocity and turbulence intensity. Measurements were made in the zone where the coolant and freestream flows mix. Flow from one row of film cooling holes with a streamwise injection of 35{degree} and no lateral injection and with a coolant- to-freestream flow velocity ratio of 1.0 was investigated under freestream turbulence levels of 0.5 and 12%. Coolant-to-freestream density ratio was unity. Two length-to-diameter ratios for the film cooling holes, 2.3 and 7.0, are tested. Results show that under low freestream turbulence conditions, pronounced differences exist in the flowfield between L/D=7.0 and 2.3; the differences are less prominent at high freestream turbulence intensities. Generally, short-L/D injection results in ``jetting`` of the coolant further into the freestream flow and enhanced mixing. Other changes in the flowfield attributable to a rise in freestream turbulence intensity to engine- representative conditions are documented. 15 figs, 2 tabs, refs.

  5. An open-loop RFOG based on harmonic division technique to suppress LD's intensity modulation noise

    NASA Astrophysics Data System (ADS)

    Ying, Diqing; Wang, Zeyu; Mao, Jianmin; Jin, Zhonghe

    2016-11-01

    A harmonic division technique is proposed for an open-loop resonator fiber optic gyro (RFOG) to suppress semiconductor laser diode's (LD's) intensity modulation noise. The theoretical study indicates the RFOG with this technique is immune to the intensity noise. The simulation and experimental results show this technique would lead to a diminished linear region, which still could be acceptable for an RFOG applied to low rotation rate detection. The tests for the gyro output signal are carried out with/without noise suppressing methods, including the harmonic division technique and previously proposed signal compensation technique. With the harmonic division technique at the rotation rate of 10 deg/s, the stability of gyro output signal is improved from 1.07 deg/s to 0.0361 deg/s, whose noise suppressing ratio is more than 3 times as that of the signal compensation technique. And especially, a 3.12 deg/s signal jump is significantly removed with the harmonic division technique; in contrast, a residual 0.36 deg/s signal jump still exists with the signal compensation technique. It is concluded the harmonic division technique does work in intensity noise suppressing under dynamic condition, and it is superior to the signal compensation technique.

  6. An investigation on LD50 and subacute hepatic toxicity of Nigella sativa seed extracts in mice.

    PubMed

    Vahdati-Mashhadian, N; Rakhshandeh, H; Omidi, A

    2005-07-01

    Nigella sativa seeds (blackseed) have been used in traditional medicine for the treatment of a variety of diseases including diarrhea and asthma, and have been shown to have various useful pharmacological effects. In this study, acute and subacute toxicity of the aqueous, methanol and chloroform extracts of the seeds have been investigated. To determine their LD50, the aqueous, methanol and chloroform extracts were administered orally, in 4 different doses, 6, 9, 14 and 21 g/kg. Mortality rate and weight changes have also been measured in all groups for 3 and 7 days, respectively. No mortality has been observed in all groups and with all doses. Methanol extracts in all doses and chloroform extract in the dose of 21 g/kg significantly decreased animals weight. Hepatic toxicity of the extracts was also investigated in the dose of 6 g/kg/day orally for 14 consecutive days by measuring ALP, SGOT and SGPT activity in blood and hepatic histological study. Degenerative changes in hepatic cells have been observed only with aqueous extract of the seeds. In conclusion, Nigella sativa extracts are relatively nontoxic in the acute toxicity test, but the possibility of hepatic damage with its aqueous extract should be considered.

  7. Multifunctional coating MAI D5 intended for the protection of refractory materials

    NASA Astrophysics Data System (ADS)

    Zhestkov, B. E.; Terent'eva, V. S.

    2010-01-01

    A thin-layer heterophase MAI D5 coating intended for the protection of carbon-containing composite materials (C-C-, C-SiC-, SiC-SiC-composite material) and refractory metal-based alloys against high-temperature gas corrosion and erosion is tested. The coating is formed from a specially developed Si-Ti-Mo-B-Y microcomposition powder material. A new approach to creating self-healing heterophase coatings is used. At operating temperatures, the structure and phase composition of the MAI D5 coating provides rapid self-healing of random defects on the coating surface and in the base material owing to the presence of a silicon-containing eutectic, which favors rapid recovery of a protective amorphous silica layer consumed during operation. Under operating conditions, the coating undergoes self-organization to form a multilayer system with a number of functional oxide micro-, submicro-, and nanolayers. Samples with the MAI D5 coating are subjected to combined tests on bench plants located at MAI and TsAGI under conditions emulating the re-entry of a hypersonic aircraft, when its surface temperature reaches 2400 K. Certification tests are performed in wind tunnels located at TsAGI during a simultaneous action of a hypersonic dissociated-air flow and mechanical loads. The protection of heat-loaded structural elements in space vehicles made of ultrahigh-temperature materials against high-temperature gas corrosion and erosion by the MAI D5 coating in hypersonic dissociated-air flows is shown to be efficient.

  8. ReVeaLD: a user-driven domain-specific interactive search platform for biomedical research.

    PubMed

    Kamdar, Maulik R; Zeginis, Dimitris; Hasnain, Ali; Decker, Stefan; Deus, Helena F

    2014-02-01

    Bioinformatics research relies heavily on the ability to discover and correlate data from various sources. The specialization of life sciences over the past decade, coupled with an increasing number of biomedical datasets available through standardized interfaces, has created opportunities towards new methods in biomedical discovery. Despite the popularity of semantic web technologies in tackling the integrative bioinformatics challenge, there are many obstacles towards its usage by non-technical research audiences. In particular, the ability to fully exploit integrated information needs using improved interactive methods intuitive to the biomedical experts. In this report we present ReVeaLD (a Real-time Visual Explorer and Aggregator of Linked Data), a user-centered visual analytics platform devised to increase intuitive interaction with data from distributed sources. ReVeaLD facilitates query formulation using a domain-specific language (DSL) identified by biomedical experts and mapped to a self-updated catalogue of elements from external sources. ReVeaLD was implemented in a cancer research setting; queries included retrieving data from in silico experiments, protein modeling and gene expression. ReVeaLD was developed using Scalable Vector Graphics and JavaScript and a demo with explanatory video is available at http://www.srvgal78.deri.ie:8080/explorer. A set of user-defined graphic rules controls the display of information through media-rich user interfaces. Evaluation of ReVeaLD was carried out as a game: biomedical researchers were asked to assemble a set of 5 challenge questions and time and interactions with the platform were recorded. Preliminary results indicate that complex queries could be formulated under less than two minutes by unskilled researchers. The results also indicate that supporting the identification of the elements of a DSL significantly increased intuitiveness of the platform and usability of semantic web technologies by domain users.

  9. Effects of MeJA on Arabidopsis metabolome under endogenous JA deficiency

    PubMed Central

    Cao, Jingjing; Li, Mengya; Chen, Jian; Liu, Pei; Li, Zhen

    2016-01-01

    Jasmonates (JAs) play important roles in plant growth, development and defense. Comprehensive metabolomics profiling of plants under JA treatment provides insights into the interaction and regulation network of plant hormones. Here we applied high resolution mass spectrometry based metabolomics approach on Arabidopsis wild type and JA synthesis deficiency mutant opr3. The effects of exogenous MeJA treatment on the metabolites of opr3 were investigated. More than 10000 ion signals were detected and more than 2000 signals showed significant variation in different genotypes and treatment groups. Multivariate statistic analyses (PCA and PLS-DA) were performed and a differential compound library containing 174 metabolites with high resolution precursor ion-product ions pairs was obtained. Classification and pathway analysis of 109 identified compounds in this library showed that glucosinolates and tryptophan metabolism, amino acids and small peptides metabolism, lipid metabolism, especially fatty acyls metabolism, were impacted by endogenous JA deficiency and exogenous MeJA treatment. These results were further verified by quantitative reverse transcription PCR (RT-qPCR) analysis of 21 related genes involved in the metabolism of glucosinolates, tryptophan and α-linolenic acid pathways. The results would greatly enhance our understanding of the biological functions of JA. PMID:27883040

  10. Effects of MeJA on Arabidopsis metabolome under endogenous JA deficiency

    NASA Astrophysics Data System (ADS)

    Cao, Jingjing; Li, Mengya; Chen, Jian; Liu, Pei; Li, Zhen

    2016-11-01

    Jasmonates (JAs) play important roles in plant growth, development and defense. Comprehensive metabolomics profiling of plants under JA treatment provides insights into the interaction and regulation network of plant hormones. Here we applied high resolution mass spectrometry based metabolomics approach on Arabidopsis wild type and JA synthesis deficiency mutant opr3. The effects of exogenous MeJA treatment on the metabolites of opr3 were investigated. More than 10000 ion signals were detected and more than 2000 signals showed significant variation in different genotypes and treatment groups. Multivariate statistic analyses (PCA and PLS-DA) were performed and a differential compound library containing 174 metabolites with high resolution precursor ion-product ions pairs was obtained. Classification and pathway analysis of 109 identified compounds in this library showed that glucosinolates and tryptophan metabolism, amino acids and small peptides metabolism, lipid metabolism, especially fatty acyls metabolism, were impacted by endogenous JA deficiency and exogenous MeJA treatment. These results were further verified by quantitative reverse transcription PCR (RT-qPCR) analysis of 21 related genes involved in the metabolism of glucosinolates, tryptophan and α-linolenic acid pathways. The results would greatly enhance our understanding of the biological functions of JA.

  11. Human Health Impact of Fluoride in Groundwater in the Chiang Mai Basin

    NASA Astrophysics Data System (ADS)

    Matsui, Y.; Takizawa, S.; Wattanachira, S.; Wongrueng, A.; Ibaraki, M.

    2005-12-01

    Chiang Mai Basin, in Northern Thailand, is known as a fluorotic area. Groundwater of the Chiang Mai Basin has been gradually replaced by contaminated surface water since the 1980's. People have been exposed to fluoride contaminated groundwater since that time. As a result, harmful health effects on dental and skeletal growth were observed in the 90's. These include dental and skeletal fluorosis. Dental fluorosis is characterized by yellow or white spots on teeth and pitting or mottled enamel, consequently causing the teeth to look unsightly. Skeletal fluorosis leads to changes in bone structure, making them extremely weak and brittle. The most severe form of this is known as ``crippling skeletal fluorosis,'' a condition that can cause immobility, muscle wasting, and neurological problems related to spinal cord compression. This study focuses on the problematic issue of the Chiang Mai Basin's groundwater from the viewpoint of fluoride occurrence and current health impacts. Chiang Mai and Lamphun Provinces comprise the Chiang Mai Basin. Fluoride rich granites or fluorite deposits are scattered across the mountainside of the Lamphun Province. Tropical savanna climate conditions with seasonal monsoons bring more than 1,000 mm of annual precipitation, which can prompt weathering of minerals containing fluoride. The Ping River dominates the Basin, and the main eastern tributary of the Ping River runs through the Lamphun Province. The Basin has geological units composed of lower semi-consolidated Tertiary fluvial and upper unconsolidated Quaternary alluvium deposits. The main aquifers are in the upper unconsolidated unit. High fluoride concentrations tend to be observed in the aquifer located in lower part of this unconsolidated unit. We have been investigating two areas in the Basin. These two locations are similar with respect to geological and hydrological settings. However, one area in which groundwater is Ca-bicarbonate dominant has a low fluoride occurrence

  12. Internal Flow Analysis of Large L/D Solid Rocket Motors

    NASA Technical Reports Server (NTRS)

    Laubacher, Brian A.

    2000-01-01

    Traditionally, Solid Rocket Motor (SRM) internal ballistic performance has been analyzed and predicted with either zero-dimensional (volume filling) codes or one-dimensional ballistics codes. One dimensional simulation of SRM performance is only necessary for ignition modeling, or for motors that have large length to port diameter ratios which exhibit an axial "pressure drop" during the early burn times. This type of prediction works quite well for many types of motors, however, when motor aspect ratios get large, and port to throat ratios get closer to one, two dimensional effects can become significant. The initial propellant grain configuration for the Space Shuttle Reusable Solid Rocket Motor (RSRM) was analyzed with 2-D, steady, axi-symmetric computational fluid dynamics (CFD). The results of the CFD analysis show that the steady-state performance prediction at the initial burn geometry, in general, agrees well with 1-D transient prediction results at an early time, however, significant features of the 2-D flow are captured with the CFD results that would otherwise go unnoticed. Capturing these subtle differences gives a greater confidence to modeling accuracy, and additional insight with which to model secondary internal flow effects like erosive burning. Detailed analysis of the 2-D flowfield has led to the discovery of its hidden 1-D isentropic behavior, and provided the means for a thorough and simplified understanding of internal solid rocket motor flow. Performance parameters such as nozzle stagnation pressure, static pressure drop, characteristic velocity, thrust and specific impulse are discussed in detail and compared for different modeling and prediction methods. The predicted performance using both the 1-D codes and the CFD results are compared with measured data obtained from static tests of the RSRM. The differences and limitations of predictions using ID and 2-D flow fields are discussed and some suggestions for the design of large L/D motors and

  13. The Didactic Model "LdL" (Lernen Durch Lehren) as a Way of Preparing Students for Communication in a Knowledge Society

    ERIC Educational Resources Information Center

    Grzega, Joachim; Schoner, Marion

    2008-01-01

    Based on studies in learning psychology, biology and education, the original technique "Lernen durch Lehren" ("LdL)" (German for "learning by teaching") has been elaborated into a meta-model ("Meta") "LdL" that aims at giving students a platform to acquire the competencies considered necessary for knowledge societies. Ninety-seven former students…

  14. The Effects of a Growth Mindset Intervention on the Beliefs about Intelligence, Effort Beliefs, Achievement Goal Orientations, and Academic Self-Efficacy of LD Students with Reading Difficulties

    ERIC Educational Resources Information Center

    Baldridge, Mary Caufield

    2010-01-01

    The overall purpose of this study was to examine the effects of a "growth mindset" intervention on the beliefs about intelligence, effort beliefs, achievement goals, and academic self-efficacy of learning disabled (LD) students with reading difficulties. The treatment group consisted of 12 high school LD students with reading difficulties. This…

  15. Comparisons of Self-Efficacy, Mood, Effort, and Hope between Students with Learning Disabilities and Their Non-LD-Matched Peers

    ERIC Educational Resources Information Center

    Lackaye, Timothy; Margalit, Malka; Ziv, Orit; Ziman, Tahel

    2006-01-01

    The goals of this study were to compare self-perceptions of self-efficacy, mood, effort, and hope between 123 adolescents with learning disabilities (LD) and a group of 123 Non-LD peers, who were matched for their level of academic performance and gender, and to explore the relations between measures of self-perception and achievement. The results…

  16. Effects of a Computerized Working Memory Training Program on Working Memory, Attention, and Academics in Adolescents with Severe LD and Comorbid ADHD: A Randomized Controlled Trial

    ERIC Educational Resources Information Center

    Gray, S. A.; Chaban, P.; Martinussen, R.; Goldberg, R.; Gotlieb, H.; Kronitz, R.; Hockenberry, M.; Tannock, R.

    2012-01-01

    Background: Youths with coexisting learning disabilities (LD) and attention deficit hyperactivity disorder (ADHD) are at risk for poor academic and social outcomes. The underlying cognitive deficits, such as poor working memory (WM), are not well targeted by current treatments for either LD or ADHD. Emerging evidence suggests that WM might be…

  17. MAI-free performance of PMU-OFDM transceiver in time-variant environment

    NASA Astrophysics Data System (ADS)

    Tadjpour, Layla; Tsai, Shang-Ho; Kuo, C.-C. J.

    2005-06-01

    An approximately multi-user OFDM transceiver was introduced to reduce the multi-access interference (MAI ) due to the carrier frequency offset (CFO) to a negligible amount via precoding by Tsai, Lin and Kuo. In this work, we investigate the performance of this precoded multi-user (PMU) OFDM system in a time-variant channel environment. We analyze and compare the MAI effect caused by time-variant channels in the PMU-OFDM and the OFDMA systems. Generally speaking, the MAI effect consists of two parts. The first part is due to the loss of orthogonality among subchannels for all users while the second part is due to the CFO effect caused by the Doppler shift. Simulation results show that, although OFDMA outperforms the PMU-OFDM transceiver in a fast time-variant environment without CFO, PMU-OFDM outperforms OFDMA in a slow time-variant channel via the use of M/2 symmetric or anti-symmetric codewords of M Hadamard-Walsh codes.

  18. Nanoporous Silicon Ignition of JA2 Propellant

    DTIC Science & Technology

    2014-06-01

    2 Figure 2. Photograph of the activated nanoporous silicon chip in the PVC container showing attached firing leads; JA2 propellant disk rests...chips, and each chip was placed singly in a transparent, flexible polyvinyl chloride ( PVC ) container. The PVC container (see figure 2) contained...room. The electrical leads on the outside of the PVC container were connected to the firing circuitry (an impressed voltage of 3 V across the chip

  19. On the effect of food magnesium level on the activity of BASP, ALAT, ASAT and LD in pig serum.

    PubMed

    Nuoranne, P

    1978-02-01

    Studies were made to investigate the effect of food Mg level on ALAT, ASAT, LD and BASP values in pigs. The following conclusions were drawn:--a high food Mg level (0.31% in ordinary pig food) can cause an increase in serum BASP values.--leg weakness, at the primary stage, is hardly caused by disturbances in bone.--an ordinary diet low in Mg may cause an abnormal rise in ALAT values in pigs.--when feeding pigs on an ordinary diet, pigs may suffer from Mg deficiency despite the fact that the food Mg content is distinctly higher than that recommended by international norms for feeding.--a prompt lowering of food Mg level can cause a manifest increase in ASAT, LD and BASP values within 2 to 3 days in pigs. Heart and liver injuries caused by low Mg diets and the individual ability of pits to utilize magnesium were discussed.

  20. Albrecht Unsöld: a Pioneer in the Interpretation of the Origin of the Cosmic Radio Emission

    NASA Astrophysics Data System (ADS)

    Wielebinski, Richard

    2012-09-01

    Albrecht Unsöld was born 20th April 1905 in Bohlheim (Württenberg) and died in 1995 in Kiel. He was appointed in 1932 at the age of only 26 to the Chair of Astronomy and Theoretical Physics at the University of Kiel. This appointment initiated a very successful development of the studies of stellar atmospheres in Kiel under his life-long direction. Unsöld developed an active cooperation with astronomers in the USA. During a Rockefeller Foundation fellowship he used the Mount Wilson 100'' telescope for spectral work. A major book published by him (Unsöld, 1938) ``Physik der Sternatmosphären (mit besonderer Berücksichtigung der Sonne)'' dealt with stellar atmospheres and became a classic in this research area. Prof. Unsöld kept the good contacts to colleagues in the USA having a visiting professor appointment in Chicago in 1939. At the Yerkes Observatory, working with Otto Struve (1888--1956), he became familiar with the observations of Karl Jansky (1905--1950) and Grote Reber (1911--2002). He immediately realised the close connection between the observed radio waves and the stellar atmospheres research. The origin of the radio emission observed by Jansky and Reber at first eluded interpretation. The major conclusion in the early days was that `the black-body radiation theory failed to account for Jansky's observations by a factor of 104 in the most favourable case'. Unsöld started his investigations based on the data available to him in 1940, at first trying to bring the free-free emission to explain the radio observations. Unsöld from that time on began to use the Effective Temperature definition as obtained from the Rayleigh--Jeans approximation. This was a great step forward, going away from the intensity definitions (mVolts/m) used by radio engineers up to that time. A paper submitted in 1944 to the German journal `Die Naturwissenschaften' was published only in 1946. In this paper the conclusion is drawn that only electron temperatures of some 100,000 K

  1. Reliability study on high-power 638nm broad stripe LD with a window-mirror structure

    NASA Astrophysics Data System (ADS)

    Yagi, Tetsuya; Mitsuyama, Hiroshi; Nishida, Takehiro; Kadoiwa, Kaoru; Kuramoto, Kyosuke

    2014-03-01

    Laser based displays, such as 10 lm to 70K lm laser projectors and laser diode (LD) backlight liquid crystal display (LCD) TVs, have attracted much attention because of the large gamut, low power consumption, and so on. Laser light sources for the displays are operated mainly under CW, and requested to be highly reliable. In this work, we will present the latest reliability study on high-power 638 nm broad stripe (BS) LD with a window-mirror structure, which is formed by using Zn diffusion into a quantum well active layer. Although the LD showed no catastrophic optical mirror degradation (COMD) even above the output of 1.6 W initially, the LDs aged at output power around 1.0 to 1.5 W showed sudden degradation during 1,000 to 4,000 hours. The duration to the failure became shorter as the power increased. Electro luminescence (EL) imaging revealed that the root cause of the sudden degradation was COMD at the front facet even though the LD had a measure to COMD. The LDs aged at 0.42 W output showed no COMD up to 6500 hours with extremely stable operation. The result also revealed that the mean time to failure due to COMD was proportional to optical density to the power of -3.2. The LDs, which had 60% small power density compared to the former, showed stable one up to 4,000 hours without COMD at 1.25 W. It is clarified that maintaining a low optical output power density is essential to develop high-power and highly reliable red BS-LDs.

  2. Levodropropizine (LD) activity in allergic asthmatic patients, challenged with ultrasonically nebulized distilled water, metacholine and allergen-induced bronchospasm.

    PubMed

    Bossi, R; Banfi, P; Filipazzi, V; Castelli, C; Braga, P C

    1994-04-01

    The antitussive compound Levodropropizine (LD) is active in animal bronchoconstriction induced by histamine and capsaicin and in man protects from bronchoconstriction induced by capsaicin. The primary objective of this study was to evaluate the mechanism of action of LD given at 60 mg t.i.d. as oral drops, for 8 days by means of specific bronchial challenges (allergens) and of aspecific challenges acting via different receptors and fibers (i.e. metacholine via cholinergic receptors and ultrasonically nebulized distilled water (UNDW) via histamine and neuropeptide release). The study design is randomized, double-blind, cross-over versus placebo in 30 allergic asthmatic patients. Baseline bronchial tone and bronchoconstrictor response to metacholine (MCh) were not modified by active treatment nor by placebo. On the contrary, in airway responsiveness to UNDW, the active treatment showed an antagonist effect against induced bronchoconstriction of 59% [activity ratio (AR) as antilog = 0.41; 95% confidence interval 0.35-0.54; p < or = 0.05] in comparison to no effect for placebo. Similarly, in airway responsiveness to specific allergen, active treatment antagonized the bronchoconstrictor effect of grass pollen by 83% and of various allergens (dermatophagoides and grass pollen) by 72%, i.e. AR of 0.17 (95% confidence interval 0.045-0.65; p < 0.01) and of 0.28 (95% confidence interval 0.07-1.04; p < 0.05), respectively. No antagonist effect was evident with placebo at all times. Besides inhibiting cough, LD is also partially effective in inhibiting bronchial hyperreactive response against specific allergen and UNDW bronchoconstriction. Hence, LD might act by partly inhibiting histamine and neuropeptide release.

  3. Blue and green up-conversion in ZBLAN Tm3+,Yb3+-codoped glasses pumped with LD 970-nm laser

    NASA Astrophysics Data System (ADS)

    Cao, Wanghe; Kan, Shidong; Zang, Chuanyi

    1998-08-01

    The quality of synthesized ZBLAN:Tm3+,Yb3+ glasses is analyzed and an efficient up-concentration in the blue and green light of Tm3+ containing different concentration ratio of Tm3+:Yb3+ demonstrated under LD 970 nm pumping. The up-conversion process of Tm3+ in ZBLAN:Tm3+,Yb3+ glasses are presented. The up-conversion in green can be limited restrained by the concentration of Yb3+.

  4. Effective L/D: A Theoretical Approach to the Measurement of Aero-Structural Efficiency in Aircraft Design

    NASA Technical Reports Server (NTRS)

    Guynn, Mark D.

    2015-01-01

    There are many trade-offs in aircraft design that ultimately impact the overall performance and characteristics of the final design. One well recognized and well understood trade-off is that of wing weight and aerodynamic efficiency. Higher aerodynamic efficiency can be obtained by increasing wing span, usually at the expense of higher wing weight. The proper balance of these two competing factors depends on the objectives of the design. For example, aerodynamic efficiency is preeminent for sailplanes and long slender wings result. Although the wing weight-drag trade is universally recognized, aerodynamic efficiency and structural efficiency are not usually considered in combination. This paper discusses the concept of "aero-structural efficiency," which combines weight and drag characteristics. A metric to quantify aero-structural efficiency, termed effective L/D, is then derived and tested with various scenarios. Effective L/D is found to be a practical and robust means to simultaneously characterize aerodynamic and structural efficiency in the context of aircraft design. The primary value of the effective L/D metric is as a means to better communicate the combined system level impacts of drag and structural weight.

  5. Comparison of ASAT, CK, CK-MB, and LD for the estimation of acute myocardial infarct size in man.

    PubMed

    Grande, P; Christiansen, C; Alstrup, K

    1983-03-14

    The purpose of this study was to set up a simple and reliable procedure for estimating acute myocardial infarct (AMI) size by measuring serum enzymes in a few daily blood samples. Peak enzyme values and estimated infarct size from one, two, or three daily samples of aspartate aminotransferase (ASAT), creatine kinase (CK), CK-MB, and lactate dehydrogenase (LD) were compared with the extent of myocardial necrosis measured at autopsy in 22 patients who died from AMI. The correlation between the extent of the necrosis measured and peak serum enzymes from one daily blood sample was highest for CK-MB (r = 0.78) and LD (r = 0.73) compared to CK (r = 0.68) and ASAT (r = 0.67). To obtain a significant correlation, however, two patients had to be excluded from the ASAT and LD analyses. No significant improvement was obtained by more frequent blood sampling. Estimation of infarct size did not improve the correlation significantly for any enzyme, although the coefficient of correlation for CK-MB increased slightly (r = 0.83). Serum CK-MB determination provides a semiquantitative estimate of infarct size, but the other enzymes may give erroneous estimates owing to lesser cardiospecificity.

  6. Albrecht Unsöld: his role in the interpretation of the origin of cosmic radio emission and in the beginning of radio astronomy in Germany

    NASA Astrophysics Data System (ADS)

    Wielebinski, Richard

    2013-03-01

    Albrecht Unsöld's career spanned over 50 years at the beginning of the 20th century. In this period atomic physics made great advances and Unsöld applied this to astrophysical questions. He came in contact with the early radio astronomy observations and devoted part of his career to the interpretation of the origin of cosmic radio waves. Although hampered by the post-war situation, Unsöld's contributions to the interpretation of cosmic radio waves were important.

  7. DISCRIMINATION 28S RIBOSOMAL GENE OF TREMATODE CERCARIAE IN SNAILS FROM CHIANG MAI PROVINCE, THAILAND.

    PubMed

    Wongsawad, Chalobol; Wongsawad, Pheravut; Sukontason, Kom; Phalee, Anawat; Noikong-Phalee, Waraporn; Chai, Jong Yil

    2016-03-01

    Trematode cercariae are commonly found in many freshwater gastropods. These cercariae can serve to identify the occurrence of such trematodes as Centrocestus formosanus, Haplorchis taichui, Haplorchoides sp, and Stellantchasmus falcatus, which are important parasites in Chiang Mai Province, Thailand. As the species of these cercariae cannot be identified accurately based on morphology, this study employed sequencing of a fragment of 28S ribosomal DNA and phylogenetic analysis to identify the trematode cercariae found in freshwater gastropods in Chiang Mai Province. Eight types of trematode cercariae were identified, namely, distome cercaria (grouped with Philophthalmus spp clade), echinostome cercaria (grouped with Echinostoma spp clade), furcocercous cercaria (grouped with Posthodiplostomum sp/Alaria taxideae/Hysteromorpha triloba clade), monostome cercaria (grouped with Catatropis indicus clade), parapleurolophocercous cercaria (grouped with Haplorchoides sp clade), pleurolophocercous cercaria (grouped with Centrocestusformosanus clade), transversotrema cercaria (grouped with Transversotrema spp clade), and xiphidiocercaria (grouped with Prosthodendrium spp clade). These results provide important information that can be used for identifying these parasites in epidemiological surveys.

  8. Stigma, social support, and treatment adherence among HIV-positive patients in Chiang Mai, Thailand.

    PubMed

    Li, Michael Jonathan; Murray, Jordan Keith; Suwanteerangkul, Jiraporn; Wiwatanadate, Phongtape

    2014-10-01

    Our study assessed the influence of HIV-related stigma on treatment adherence among people living with HIV in Chiang Mai, Thailand, and whether social support had a moderating effect on this relationship. We recruited 128 patients living with HIV from Sansai Hospital, a community hospital in Chiang Mai, Thailand, and collected data through structured interviews. All forms of HIV-related stigma considered in this study (personalized experience, disclosure, negative self-image, and public attitudes) were negatively correlated with adherence to anti-retroviral regimens. Multiple linear regression indicated that total HIV-related stigma was more predictive of treatment adherence than any individual stigma type, after adjusting for socio-demographic and health characteristics. Tests of interaction showed that social support did not appear to moderate the association between HIV stigma and treatment adherence. Our findings suggest that community and government efforts to improve public perceptions about people living with HIV might promote treatment adherence behaviors among HIV-positive patients.

  9. STIGMA, SOCIAL SUPPORT, AND TREATMENT ADHERENCE AMONG HIV-POSITIVE PATIENTS IN CHIANG MAI, THAILAND

    PubMed Central

    Li, Michael Jonathan; Murray, Jordan Keith; Suwanteerangkul, Jiraporn; Wiwatanadate, Phongtape

    2016-01-01

    Our study assessed the influence of HIV-related stigma on treatment adherence among people living with HIV in Chiang Mai, Thailand, and whether social support had a moderating effect on this relationship. We recruited 128 patients living with HIV from Sansai Hospital, a community hospital in Chiang Mai, Thailand, and collected data through structured interviews. All forms of HIV-related stigma considered in this study (personalized experience, disclosure, negative self-image, and public attitudes) were negatively correlated with adherence to anti-retroviral regimens. Multiple linear regression indicated that total HIV-related stigma was more predictive of treatment adherence than any individual stigma type, after adjusting for socio-demographic and health characteristics. Tests of interaction showed that social support did not appear to moderate the association between HIV stigma and treatment adherence. Our findings suggest that community and government efforts to improve public perceptions about people living with HIV might promote treatment adherence behaviors among HIV-positive patients. PMID:25299810

  10. Detection of environmental sources of Histoplasma capsulatum in Chiang Mai, Thailand, by nested PCR.

    PubMed

    Norkaew, Treepradab; Ohno, Hideaki; Sriburee, Pojana; Tanabe, Koichi; Tharavichitkul, Prasit; Takarn, Piyawan; Puengchan, Tanpalang; Bumrungsri, Sara; Miyazaki, Yoshitsugu

    2013-12-01

    Histoplasmosis is a systemic mycosis caused by inhaling spores of Histoplasma capsulatum, a dimorphic fungus. This fungus grows in soil contaminated with bat and avian excreta. Each year, patients with disseminated histoplasmosis have been diagnosed in Chiang Mai, northern Thailand. No published information is currently available on the environmental sources of this fungus in Chiang Mai or anywhere else in Thailand. The aim of this study was to detect H. capsulatum in soil samples contaminated with bat guano and avian droppings by nested PCR. Two hundred and sixty-five samples were collected from the following three sources: soil contaminated with bat guano, 88 samples; soil contaminated with bird droppings, 86 samples; and soil contaminated with chicken droppings, 91 samples. Genomic DNA was directly extracted from each sample, and H. capsulatum was detected by nested PCR using a primer set specific to a gene encoding 100-kDa-like protein (HcI, HcII and HcIII, HcIV). Histoplasma capsulatum was detected in seven of 88 soil samples contaminated with bat guano, one of 21 soil samples contaminated with pigeon droppings and 10 of 91 soil samples contaminated with chicken droppings. The results indicate the possibility of the association of bat guano and chicken droppings with H. capsulatum in this area of Thailand.

  11. The Mais Médicos (More Doctors) Program in Mato Grosso State, Brazil: implementation analysis.

    PubMed

    Mota, Reinaldo Gaspar da; Barros, Nelson Filice

    2016-09-01

    Although the 224 health professionals engaged by the More Doctors for Brazil Project (Projeto Mais Médicos para o Brazil, PMMB) to strengthen the National Primary Health Care Policy (Política Nacional de Atenção Básica, PNab) in 104 municipalities of Mato Grosso (MT) State have encountered significant problems in the work process, important advances have resulted in the health of populations served by the More Doctors Programme (Programa Mais Médicos, PMM). This article analyses the implementation and development of the PMM in MT, from 2013 to 2015, on the basis of primary data from focus groups and interviews of social stakeholders at the institutions involved and secondary data from reports by supervisors, tutors, managers and institutions on the MT State Coordinating Committee (CCE) of the PMM. Despite political difficulties in managing implementation, the results show that the endeavour was beneficial in that it surmounted previous obstacles, afforded users greater access, prompted discussion of the problem and proposed and experimented with ways to strengthen primary health care. We conclude that there is a need for broader academic discussion of the provision and training of doctors, of the model of care and of human resource capacity-building by continuing professional development with integration among teaching, service and community.

  12. The Mais Médicos (More Doctors) Program: panorama of the scientific output.

    PubMed

    Kemper, Elisandréa Sguario; Mendonça, Ana Valeria Machado; Sousa, Maria Fátima de

    2016-09-01

    Despite the progress achieved by the Primary Health Care and Family Health Strategy in the Unified Health System (SUS) challenges still remain with regard to the universality of access and the quality of services, one of the factors being the unequal distribution of physicians. The Brazilian Government established the Mais Médicos Program (More Doctors Program), in order to move forward in the provision, placement and training of physicians in the SUS. This study consists of a review of the literature of the Mais Médicos Program, in order to map and assess the scientific production on the Program, as well as summarize the findings and present the results of the analysis. Fifty-four publications were selected, which evaluate the Program in terms of effectiveness, analysis of the implementation process, the media and the statements of the actors and assessment of the legal and constitutional precepts. The criticisms and limitations found were also systematically analyzed. With respect to the analysis, evaluations of the Program are overwhelmingly positive, showing important changes in the work processes in services and training. The studies that show the Program as being an important instrument for the effective implementation of the right to health are highlighted.

  13. Simulium (Nevermannia) chomthongense, a new species of black fly (Diptera: Simuliidae) from Chiang Mai, Thailand.

    PubMed

    Takaoka, H; Srisuka, W; Saeung, A; Otsuka, Y; Choochote, W

    2012-09-01

    Simulium (Nevermannia) chomthongense sp. nov. is described from female, male, pupal and larval specimens collected from Doi Inthanon National Park and Doi Phahompok National Park, Chiang Mai, Thailand. This new species, first reported as S. (Eusimulium) sp. A, and later regarded as S. (N.) caudisclerum Takaoka & Davies, described from peninsular Malaysia, is distinguished from S. (N.) caudisclerum in the male by the number of enlarged upper-eye facets and the relative size of the hind basitarsus against the hind tibia and femur, and in the pupa by the relative length of the stalks of paired filaments against the common basal stalk and the color of the dorsal surface of abdominal segments 1- 3 (or 4). Taxonomic and molecular notes are provided to separate this new species from four other known species of the vernum species-group, which share an accessory sclerite on the larval abdomen, a rare characteristic in this species-group.

  14. Simulium (Nevermannia) khunklangense, a new species of black fly (Diptera: Simuliidae) from Chiang Mai, Thailand.

    PubMed

    Takaoka, Hiroyuki; Srisuka, Wichai; Saeung, Atiporn; Otsuka, Yasushi; Choochote, Wej

    2013-01-01

    Simulium (Nevermannia) khunklangense sp. nov. is described from females, males, pupae and larvae collected in Doi Inthanon National Park, Chiang Mai, Thailand. This new species is placed in the vernum species-group of the subgenus Nevermannia and is similar to S. (N.) chomthongense Takaoka & Srisuka described from Doi Inthanon National Park, Thailand, but is distinguished in the male by the number of enlarged upper-eye facets and the relative width of the hind basitarsus against the hind tibia and femur, and in the pupa by the short common basal stalk of the gill and the cocoon with an anterodorsal bulge or a short anterodorsal projection. Taxonomic notes are provided to separate this new species from five other known species of the vernum species-group, which share an accessory sclerite on the larval abdomen, a rare characteristics in this species-group.

  15. Bunch compression efficiency of the femtosecond electron source at Chiang Mai University

    NASA Astrophysics Data System (ADS)

    Thongbai, C.; Kusoljariyakul, K.; Saisut, J.

    2011-07-01

    A femtosecond electron source has been developed at the Plasma and Beam Physics Research Facility (PBP), Chiang Mai University (CMU), Thailand. Ultra-short electron bunches can be produced with a bunch compression system consisting of a thermionic cathode RF-gun, an alpha-magnet as a magnetic bunch compressor, and a linear accelerator as a post acceleration section. To obtain effective bunch compression, it is crucial to provide a proper longitudinal phase-space distribution at the gun exit matched to the subsequent beam transport system. Via beam dynamics calculations and experiments, we investigate the bunch compression efficiency for various RF-gun fields. The particle distribution at the RF-gun exit will be tracked numerically through the alpha-magnet and beam transport. Details of the study and results leading to an optimum condition for our system will be presented.

  16. Construction and performance of the magnetic bunch compressor for the THz facility at Chiang Mai University

    NASA Astrophysics Data System (ADS)

    Saisut, J.; Kusoljariyakul, K.; Rimjaem, S.; Kangrang, N.; Wichaisirimongkol, P.; Thamboon, P.; Rhodes, M. W.; Thongbai, C.

    2011-05-01

    The Plasma and Beam Physics Research Facility at Chiang Mai University has established a THz facility to focus on the study of ultra-short electron pulses. Short electron bunches can be generated from a system that consists of a radio-frequency (RF) gun with a thermionic cathode, an alpha magnet as a magnetic bunch compressor, and a linear accelerator as a post-acceleration section. The alpha magnet is a conventional and simple instrument for low-energy electron bunch compression. With the alpha magnet constructed in-house, several hundred femtosecond electron bunches for THz radiation production can be generated from the thermionic RF gun. The construction and performance of the alpha magnet, as well as some experimental results, are presented in this paper.

  17. Implementation of integrated heterogeneous electronic electrocardiography data into Maharaj Nakorn Chiang Mai Hospital Information System.

    PubMed

    Khumrin, Piyapong; Chumpoo, Pitupoom

    2016-03-01

    Electrocardiography is one of the most important non-invasive diagnostic tools for diagnosing coronary heart disease. The electrocardiography information system in Maharaj Nakorn Chiang Mai Hospital required a massive manual labor effort. In this article, we propose an approach toward the integration of heterogeneous electrocardiography data and the implementation of an integrated electrocardiography information system into the existing Hospital Information System. The system integrates different electrocardiography formats into a consistent electrocardiography rendering by using Java software. The interface acts as middleware to seamlessly integrate different electrocardiography formats. Instead of using a common electrocardiography protocol, we applied a central format based on Java classes for mapping different electrocardiography formats which contains a specific parser for each electrocardiography format to acquire the same information. Our observations showed that the new system improved the effectiveness of data management, work flow, and data quality; increased the availability of information; and finally improved quality of care.

  18. Autologous chondrocyte implantation for cartilage injury treatment in Chiang Mai University Hospital: a case report.

    PubMed

    Wongtriratanachai, Prasit; Pruksakorn, Dumnoensun; Pothacharoen, Peraphan; Nimkingratana, Puwapong; Pattamapaspong, Nuttaya; Phornphutkul, Chanakarn; Setsitthakun, Sasiwariya; Fongsatitkul, Ladda; Phrompaet, Sureeporn

    2013-11-01

    Autologous chondrocyte implantation (ACI) has become one of the standard procedures for articular cartilage defect treatment. This technique provides a promising result. However the procedural process requires an approach of several steps from multidisciplinary teams. Although the success of this procedure has been reported from Srinakharinvirot University since 2007, the application of ACI is still limited in Thailand due to the complexity of processes and stringent quality control. This report is to present the first case of the cartilage defect treatment using the first generation-ACI under Chiang Mai University's (CMU) own facility and Ethics Committee. This paper also reviews the process of biotechnology procedures, patient selection, surgical, and rehabilitation techniques. The success of the first case is an important milestone for the further development of the CMU Human Translational Research Laboratory in near future.

  19. The significance of matrix effects on the measurement of lactate dehydrogenase (LD) activity using Kodak dry slide technology in the Ontario Laboratory Proficiency Testing Program.

    PubMed

    Hill, S A; Heathcote, J C; McQueen, M J

    1990-04-01

    A recent lactate dehydrogenase (LD) survey of the Laboratory Proficiency Testing Program (LPTP) of Ontario showed interlaboratory coefficients of variation ranging from 6.5% to 40% for five lyophilized vials on the 12 Kodak analyzers. All the LPTP survey samples had similar protein and LD isoenzyme electrophoretic patterns which remained unchanged after reconstitution and storage for 5 days at 4 degrees C, although the total LD activities fell. Four Ektachem 700 analyzers were subsequently tested using LPTP material and no difference in LD activity between instruments or between two LD slide lot numbers was shown. Generation 9 slides gave higher LD activities than generation 10 on all the reconstituted lyophilized proficiency testing samples. There was no significant difference between slide generations when 19 liquid human sera were analyzed, indicating that the variability on LPTP samples was due to a matrix effect. Definition of the matrix effect of lyophilized proficiency testing material is essential before any proficiency testing program can use such material to reflect analytical performance on patient specimens.

  20. Hydrogeological features and environmental impacts of geothermal waters in the Yıldız River Basin (Sivas, Turkey).

    PubMed

    Keskin, Tülay Ekemen; Kaçaroğlu, Fikret

    2015-02-01

    The study area, located in the Yıldız River Basin (Sivas), has eight geothermal waters, which are used for balneotherapy. The aim of this study was to investigate the natural and/or anthropogenic water pollution in terms of drinking and irrigation water in the Sıcak Çermik, Uyuz Çermiği, and Hamzaşeyh Çermiği geothermal areas. Na+, Cl-, SO4(-2), B, Mn, Fe, As, and Se concentrations of the geothermal waters generally exceed the upper limits stated in the Turkish Standards for Water Intended for Human Consumption and World Health Organization regulations. Based on the irrigation water classification by the U.S. Salinity Laboratory Diagram, it is concluded that these waters may have hazardous levels of salinity and sodium when used as irrigation water. Wastewater from these health and tourism centers is discharged into the Yıldız River and to agricultural land. This procedure causes increase in the concentrations of major and minor elements and negatively affects the river water quality.

  1. Analysis of ligand-protein exchange by Clustering of Ligand Diffusion Coefficient Pairs (CoLD-CoP).

    PubMed

    Snyder, David A; Chantova, Mihaela; Chaudhry, Saadia

    2015-06-01

    NMR spectroscopy is a powerful tool in describing protein structures and protein activity for pharmaceutical and biochemical development. This study describes a method to determine weak binding ligands in biological systems by using hierarchic diffusion coefficient clustering of multidimensional data obtained with a 400 MHz Bruker NMR. Comparison of DOSY spectrums of ligands of the chemical library in the presence and absence of target proteins show translational diffusion rates for small molecules upon interaction with macromolecules. For weak binders such as compounds found in fragment libraries, changes in diffusion rates upon macromolecular binding are on the order of the precision of DOSY diffusion measurements, and identifying such subtle shifts in diffusion requires careful statistical analysis. The "CoLD-CoP" (Clustering of Ligand Diffusion Coefficient Pairs) method presented here uses SAHN clustering to identify protein-binders in a chemical library or even a not fully characterized metabolite mixture. We will show how DOSY NMR and the "CoLD-CoP" method complement each other in identifying the most suitable candidates for lysozyme and wheat germ acid phosphatase.

  2. Analysis of ligand-protein exchange by Clustering of Ligand Diffusion Coefficient Pairs (CoLD-CoP)

    NASA Astrophysics Data System (ADS)

    Snyder, David A.; Chantova, Mihaela; Chaudhry, Saadia

    2015-06-01

    NMR spectroscopy is a powerful tool in describing protein structures and protein activity for pharmaceutical and biochemical development. This study describes a method to determine weak binding ligands in biological systems by using hierarchic diffusion coefficient clustering of multidimensional data obtained with a 400 MHz Bruker NMR. Comparison of DOSY spectrums of ligands of the chemical library in the presence and absence of target proteins show translational diffusion rates for small molecules upon interaction with macromolecules. For weak binders such as compounds found in fragment libraries, changes in diffusion rates upon macromolecular binding are on the order of the precision of DOSY diffusion measurements, and identifying such subtle shifts in diffusion requires careful statistical analysis. The "CoLD-CoP" (Clustering of Ligand Diffusion Coefficient Pairs) method presented here uses SAHN clustering to identify protein-binders in a chemical library or even a not fully characterized metabolite mixture. We will show how DOSY NMR and the "CoLD-CoP" method complement each other in identifying the most suitable candidates for lysozyme and wheat germ acid phosphatase.

  3. An analysis of the impact of cabin floor angle restrictions on L/D for a typical supersonic transport

    NASA Technical Reports Server (NTRS)

    Radkey, R. L.

    1974-01-01

    High floor angles at cruise have been identified as a significant problem facing airline and public acceptance of a supersonic transport. In order to explore the relationship between cruise performances and floor angle, four related wing-fuselage design and integration studies have been conducted. The studies were: (1) a fuselage camber study in which perturbations in the fuselage camber distribution were examined with a baseline wing, (2) a wing optimization study in which wings were optimized for minimum drag at C sub L's less than the design C sub L. These wings were optimized as wing planform camber surfaces alone and evaluated with a baseline fuselage, (3) a second wing optimization study in which wings were optimized for minimum drag at C sub L's less than the design C sub L but for this study the wings were optimized in the presence of the baseline fuselage, and (4) a third wing optimization study in which wings were optimized for minmum drag subject to C sub M constraints designed to produce more positive C sub MO's, thereby reducing trim drag. The studies indicated that it was not possible to both improve the aircraft cruise L/D and substantially reduce the cruise floor angle. The studies did indicate that the cruise floor angle could be reduced by reducing the fuselage incidence relative to the wing, but the reduction in floor angle was accompanied by a substantial reduction in L/D.

  4. Endogenous Bioactive Jasmonate Is Composed of a Set of (+)-7-iso-JA-Amino Acid Conjugates.

    PubMed

    Yan, Jianbin; Li, Suhua; Gu, Min; Yao, Ruifeng; Li, Yuwen; Chen, Juan; Yang, Mai; Tong, Jianhua; Xiao, Langtao; Nan, Fajun; Xie, Daoxin

    2016-12-01

    Jasmonates (JAs) regulate a wide range of plant defense and development processes. The bioactive JA is perceived by its receptor COI1 to trigger the degradation of JASMONATE ZIM-DOMAIN (JAZ) proteins and subsequently derepress the JAZ-repressed transcription factors for activation of expression of JA-responsive genes. So far, (+)-7-iso-JA-l-Ile has been the only identified endogenous bioactive JA molecule. Here, we designed coronafacic acid (CFA) conjugates with all the amino acids (CFA-AA) to mimic the JA amino acid conjugates, and revealed that (+)-7-iso-JA-Leu, (+)-7-iso-JA-Val, (+)-7-iso-JA-Met, and (+)-7-iso-JA-Ala are new endogenous bioactive JA molecules. Furthermore, our studies uncover the general characteristics for all the bioactive JA molecules, and provide a new strategy to synthetically generate novel active JA molecules.

  5. Questões mais freqüentes na área de astronomia

    NASA Astrophysics Data System (ADS)

    Segundo, H. A. S.; Garcia, G. C.; Caretta, C. A.; Lima, F. P.

    2003-08-01

    É amplamente reconhecido que as pessoas em geral têm grande fascínio e interesse pela Astronomia. Por outro lado, o conteúdo dessa área incluído no ensino formal está longe de ser abrangente e suficiente para suprir a demanda. Esse interesse permite otimizar a divulgação e o ensino não formal de Astronomia aliando a adequação do conteúdo à expectativa das pessoas. Nesse intuito, nosso trabalho busca mapear que assuntos da Astronomia despertam mais a atenção e o interesse das pessoas e o quanto disso é estimulado pela mídia, que freqüentemente aproveita-se desse interesse de forma sensacionalista, trazendo contribuições positivas e negativas. Para esta avaliação, utilizamos as pesquisas específicas e de caracterização do público que freqüentou o MAst nos anos de 2001 a 2003, pesquisa das matérias de Astronomia veiculadas na mídia escrita da cidade do Rio de Janeiro no mesmo período, além da base de dados do programa Pergunte a um astrônomo, realizado no Observatório Nacional nos anos de 1997 a 1999. Para a análise dos dados, dividimos as perguntas em dez categorias, cada uma com suas subcategorias, tomando como referência as divisões, comissões e grupos de trabalho da IAU. Apresentamos neste trabalho os resultados dessa pesquisa, que incluem, entre outros, a predominância de questões nas categorias Observação do Céu e Sistemas Planetários enquanto nos jornais dominam as notícias de Sistemas Planetários e Espaço & Astronáutica. Outro resultado interessante indicou que as dúvidas de crianças do ensino fundamental se concentram em algumas categorias enquanto as questões de adultos são mais distribuídas por todas as categorias. Os resultados dessa pesquisa serão aplicados diretamente no aperfeiçoamento das atividades do Programa de Observação de Céu, bem como na elaboração de novos projetos e eventos realizados no MAst.

  6. Paxillin LD4 motif binds PAK and PIX through a novel 95-kD ankyrin repeat, ARF-GAP protein: A role in cytoskeletal remodeling.

    PubMed

    Turner, C E; Brown, M C; Perrotta, J A; Riedy, M C; Nikolopoulos, S N; McDonald, A R; Bagrodia, S; Thomas, S; Leventhal, P S

    1999-05-17

    Paxillin is a focal adhesion adaptor protein involved in the integration of growth factor- and adhesion-mediated signal transduction pathways. Repeats of a leucine-rich sequence named paxillin LD motifs (Brown M.C., M.S. Curtis, and C.E. Turner. 1998. Nature Struct. Biol. 5:677-678) have been implicated in paxillin binding to focal adhesion kinase (FAK) and vinculin. Here we demonstrate that the individual paxillin LD motifs function as discrete and selective protein binding interfaces. A novel scaffolding function is described for paxillin LD4 in the binding of a complex of proteins containing active p21 GTPase-activated kinase (PAK), Nck, and the guanine nucleotide exchange factor, PIX. The association of this complex with paxillin is mediated by a new 95-kD protein, p95PKL (paxillin-kinase linker), which binds directly to paxillin LD4 and PIX. This protein complex also binds to Hic-5, suggesting a conservation of LD function across the paxillin superfamily. Cloning of p95PKL revealed a multidomain protein containing an NH2-terminal ARF-GAP domain, three ankyrin-like repeats, a potential calcium-binding EF hand, calmodulin-binding IQ motifs, a myosin homology domain, and two paxillin-binding subdomains (PBS). Green fluorescent protein- (GFP-) tagged p95PKL localized to focal adhesions/complexes in CHO.K1 cells. Overexpression in neuroblastoma cells of a paxillin LD4 deletion mutant inhibited lamellipodia formation in response to insulin-like growth fac- tor-1. Microinjection of GST-LD4 into NIH3T3 cells significantly decreased cell migration into a wound. These data implicate paxillin as a mediator of p21 GTPase-regulated actin cytoskeletal reorganization through the recruitment to nascent focal adhesion structures of an active PAK/PIX complex potentially via interactions with p95PKL.

  7. Investigating a Correlation between Chemoradiotherapy Schedule Parameters and Overall Survival in a real-life LD SCLC Patient Cohort

    PubMed Central

    Manapov, Farkhad; Eze, Chukwuka; Niyazi, Maximilian; Roengvoraphoj, Olarn; Li, Minglun; Hegemann, Nina-Sophie; Hildebrandt, Guido; Fietkau, Rainer; Belka, Claus

    2016-01-01

    Background: Chemoradiotherapy (CRT) is a treatment standard in limited disease (LD) small cell lung cancer (SCLC). Currently, the timing of thoracic radiation therapy (TRT) remains the subject of randomised trials and meta-analyses. To investigate a correlation between CRT schedule parameters and overall survival (OS) in a real-life patient cohort, a temporal analysis was performed. Methods: 182 LD SCLC patients successfully treated with definitive CRT were retrospectively reviewed. TRT was applied concurrently or sequentially. Impact of the treatment mode and interval of simultaneous treatment (IST) (an interval in days when chemotherapy and TRT were applied simultaneously, including time between chemotherapy cycles and weekends) on OS was analysed. Results: 71 (39%) patients were treated with concurrent and 111 (61%) with sequential CRT. Median overall survival (MS) for the entire cohort was 534 days (95%CI 461 - 607) without any significant difference between the concurrent and sequential groups (589: 95%CI 358 - 820 vs. 533: 95%CI 446 - 620 days, p=0.746, log-rank test). IST was 0 days in 111 (61%) patients treated sequentially whereas in the concurrent group, 20 (11%) and 51 (28%) patients showed an IST < 35 and > 35 days, respectively. Patients with IST > 0 and < 35 days demonstrated a trend to improved overall survival (MS: IST 0 vs. > 35 vs. < 35 was 533 vs. 448 vs. 1169 days, p=0.109, log-rank test). When patients treated with sequential CRT (IST 0) were excluded from the analysis, statistical difference in overall survival according to the IST subgroups (IST > 35 vs. < 35) became significant (p=0.021, log-rank test). On multivariate analysis of patients treated with concurrent CRT, IST > 0 and < 35 days remained a variable that significantly correlated with better overall survival (p=0.039, HR 0.38). Conclusion: In this real-life LD SCLC patient cohort, improved overall survival was achieved in patients treated with CRT schedule according to the IST > 0

  8. Molecular Phylogenetics of Centrocestus formosanus (Digenea: Heterophyidae) Originated from Freshwater Fish from Chiang Mai Province, Thailand.

    PubMed

    Wongsawad, Chalobol; Wongsawad, Pheravut; Sukontason, Kom; Maneepitaksanti, Worawit; Nantarat, Nattawadee

    2017-02-01

    This study aimed to investigate the morphology and reconstruct the phylogenetic relationships of Centrocestus formosanus originating from 5 species of freshwater fish, i.e., Esomus metallicus, Puntius brevis, Anabas testudineus, Parambassis siamensis, and Carassius auratus, in Chiang Mai province, Thailand. Sequence-related amplified polymorphism (SRAP) and phylogeny based on internal transcribed spacer 2 (ITS2) and mitochondrial cytochrome c oxidase subunit 1 (CO1) were performed. The results showed similar morphologies of adult C. formosanus from day 5 after infection in chicks. C. formosanus originated from 4 species of freshwater fish had the same number of circumoral spines on the oral sucker, except for those from C. auratus which revealed 34 circumoral spines. The phylogenetic tree obtained from SRAP profile and the combination of ITS2 and CO1 sequence showed similar results that were correlated with the number of circumoral spines in adult worms. Genetic variability of C. formosanus also occurred in different species of freshwater fish hosts. However, more details of adult worm morphologies and more sensitive genetic markers are needed to confirm the species validity of C. formosanus with 34 circumoral spines originating from C. auratus in the future.

  9. Molecular Phylogenetics of Centrocestus formosanus (Digenea: Heterophyidae) Originated from Freshwater Fish from Chiang Mai Province, Thailand

    PubMed Central

    Wongsawad, Chalobol; Wongsawad, Pheravut; Sukontason, Kom; Maneepitaksanti, Worawit; Nantarat, Nattawadee

    2017-01-01

    This study aimed to investigate the morphology and reconstruct the phylogenetic relationships of Centrocestus formosanus originating from 5 species of freshwater fish, i.e., Esomus metallicus, Puntius brevis, Anabas testudineus, Parambassis siamensis, and Carassius auratus, in Chiang Mai province, Thailand. Sequence-related amplified polymorphism (SRAP) and phylogeny based on internal transcribed spacer 2 (ITS2) and mitochondrial cytochrome c oxidase subunit 1 (CO1) were performed. The results showed similar morphologies of adult C. formosanus from day 5 after infection in chicks. C. formosanus originated from 4 species of freshwater fish had the same number of circumoral spines on the oral sucker, except for those from C. auratus which revealed 34 circumoral spines. The phylogenetic tree obtained from SRAP profile and the combination of ITS2 and CO1 sequence showed similar results that were correlated with the number of circumoral spines in adult worms. Genetic variability of C. formosanus also occurred in different species of freshwater fish hosts. However, more details of adult worm morphologies and more sensitive genetic markers are needed to confirm the species validity of C. formosanus with 34 circumoral spines originating from C. auratus in the future. PMID:28285504

  10. Mais Médicos (More Doctors) Program - a view from England.

    PubMed

    Harris, Matthew

    2016-09-01

    The Programa Mais Medicos (PMM) is a national strategy to increase the numbers of Brazilian trained doctors entering primary care and is possibly the most significant human resource intervention in Latin America in recent years. From an English perspective, there are clearly opportunities to learn the PMM. First, PAHO's role in the PMM provides an exemplar for an overarching human resource migration and recruitment role throughout the EU. The role of the WHO in influencing and overseeing the recruitment of doctors throughout the EU could be an opportunity for improved distribution, avoiding a reliance on market forces. Secondly, a centrally-coordinated and governed process following well-established criteria and guidance laid out in law has helped to ensure that doctors are allocated to regions of the greatest need. Finally, the deployment of primary care doctors to ensure that the needs of the whole population are met, including in hard-to-reach areas. However, Brazil should not fall into the trap of doing much, and evaluating little. Brazil is in an exciting position to conduct robust before-after studies regarding the improvement in access, outcomes and equity that the ESF has already been credited with. Evaluation must include the impact of the PMM on Cuba.

  11. Coital Experience Among Adolescents in Three Social-Educational Groups in Urban Chiang Mai, Thailand

    PubMed Central

    Tangmunkongvorakul, Arunrat; Carmichael, Gordon; Banwell, Cathy; Seubsman, Sam-ang; Sleigh, Adrian

    2012-01-01

    This article compares coital experience of Chiang Mai 17–20-year-olds who were: (1) out-of-school; (2) studying at vocational schools; and (3) studying at general schools or university. Four-fifths, two-thirds and one-third, respectively, of males in these groups had had intercourse, compared to 53, 62 and 15 per cent of females. The gender difference for general school/university students, but not vocational school students, probably reflects HIV/AIDS refocusing male sexual initiation away from commercial sex workers. Vocational school females may have been disproportionately affected. Loss of virginity was associated, for both sexes, with social-educational background and lifestyle, and was less likely in certain minority ethnic groups. Among males, it was also associated with age and parental marital dissolution, and among females, with independent living and parental disharmony. Within social-educational groups, lifestyle variables dominated, but among general school/university students, parental marital dissolution (for males) and disharmony (for females) were also important, and Chinese ethnicity deterred male sexual experimentation. PMID:22754584

  12. Associations between damage location and five main body region injuries of MAIS 3–6 injured occupants

    PubMed Central

    Tang, Youming; Cao, Libo; Kan, Steven

    2014-01-01

    Objectives To examine the damage location distribution of five main body region injuries of maximum abbreviated injury score (MAIS) 3–6 injured occupants for nearside struck vehicle in front-to-side impact crashes. Design and setting MAIS 3–6 injured occupants information was extracted from the US-National Automotive Sampling System/Crashworthiness Data System in the year 2007; it included the head/face/neck, chest, pelvis, upper extremity and lower extremity. Struck vehicle collision damage was classified in a three-dimensional system according to the J224 Collision Deformation Classification of SAE Surface Vehicle Standard. Participants Nearside occupants seated directly adjacent to the struck side of the vehicle with MAIS 3–6 injured, in light truck vehicles–passenger cars (LTV–PC) side impact crashes. Outcome measures Distribution of MAIS 3–6 injured occupants by body regions and specific location of damage (lateral direction, horizontal direction and vertical direction) were examined. Injury risk ratio was also assessed. Results The lateral crush zone contributed to MAIS 3–6 injured occupants (n=705) and 50th centile injury risks when extended into zone 3. When the crush extended to zone 4, the injury risk ratio of MAIS 3–6 injured occupants approached 81%. The horizontal crush zones contributing to the highest injury risk ratio of MAIS 3–6 occupants were zones ‘D’ and ‘Y’, and the injury risk ratios were 25.4% and 36.9%, respectively. In contrast, the lowest injury risk ratio was 5.67% caused by zone ‘B’. The vertical crush zone which contributed to the highest injury risk ratio of MAIS 3–6 occupants was zone ‘E’, whose injury risk ratio was 58%. In contrast, the lowest injury risk ratio was 0.14% caused by zone ‘G+M’. Conclusions The highest injury risk ratio of MAIS 3–6 injured occupants caused by crush intrusion between 40 and 60 cm in LTV–PC nearside impact collisions and the damage region of the struck

  13. Alum adjuvanted rabies DNA vaccine confers 80% protection against lethal 50 LD50 rabies challenge virus standard strain.

    PubMed

    Garg, Rajni; Kaur, Manpreet; Saxena, Ankur; Prasad, Rajendra; Bhatnagar, Rakesh

    2017-03-03

    Rabies is a serious concern world-wide. Despite availability of rabies vaccines for long; their efficacy, safety, availability and cost effectiveness has been a tremendous issue. This calls for improvement of rabies vaccination strategies. DNA vaccination has immense potential in this regard. The DNA vaccine pgp.LAMP-1 conferred 60% protection to BALB/c mice against 20 LD50 rabies challenge virus standard (CVS) strain challenge. Upon supplementation with Emulsigen-D, the vaccine formulation conferred complete protection against lethal challenge. To assess the feasibility of this vaccine formulation for human use, it was tested along with other FDA approved adjuvants, namely, Alum, Immuvac, Montanide ISA720 VG. Enhanced immune response correlated with high IgG antibody titer, Th2 biased response with a high level of rabies virus neutralizing antibodies (RVNAs) and IgG1/IgG2a ratio >1, observed upon alum supplementation of the rabies DNA vaccine. The total IgG antibody titer was 2IU/ml and total RVNA titer was observed to be 4IU/ml which is eight times higher than the minimum protective titer recommended by WHO. Furthermore, it conferred 80% protection against challenge with 50 LD50 of the rabies CVS strain, conducted in compliance with the potency test for rabies recommended by the National Institutes of Health (NIH), USA. Previously, we have established pre-clinical safety of this vaccine as per the guidelines of Schedule Y, FDA as well as The European Agency for evaluation of Medicinal Products. The vaccine showed no observable toxicity at the site of injection as well as at systemic level in Wistar rats when administered with 10X recommended dose. Therefore, supplementation of rabies DNA vaccine, pgp.LAMP-1 with alum would lead to development of a non-toxic, efficacious, stable and affordable vaccine that can be used to combat high numbers of fatal rabies infections tormenting developing countries.

  14. Primary Health Care in Brazil and the Mais Médicos (More Doctors) Program: an analysis of production indicators.

    PubMed

    Lima, Rodrigo Tobias de Sousa; Fernandes, Tiotrefis Gomes; Balieiro, Antônio Alcirley da Silva; Costa, Felipe Dos Santos; Schramm, Joyce Mendes de Andrade; Schweickardt, Julio Cesar; Ferla, Alcindo Antonio

    2016-09-01

    This study analyzes the number of medical appointments and referrals performed in primary health care in Brazil focusing on the Mais Médicos Program (More Doctors Program). It is a cross-sectional study on the work of physicians included, or not, to the Mais Médicos Program in 2014. Based on validation protocols, a unified database was created from two health information system databases - SIAB and ESUS. Absolute indicators were defined: the total of medical appointments per month; medical referrals and community health education activities. In addition, other indicators were considered, such as weekly rates and productivity of appointments, in line with the profile of Brazilian municipalities. The mean of all appointments was 285 per month corresponding to an average of 14.4 appointments/day. In the poorest municipalities, the figures for the Mais Médicos Program physicians were higher than national rates. The educational activities provided by primary care teams that included a Mais Médicos Program professional were higher in Brazilian capital cities. The Mais Médicos Program achieved one of its main goals, which was to increase health access for vulnerable populations and to contribute towards the consolidation of primary health care in Brazil.

  15. Measurement of slow-moving along-track displacement from an efficient multiple-aperture SAR interferometry (MAI) stacking

    USGS Publications Warehouse

    Jo, Min-Jeong; Jung, Hyung-Sup; Won, Joong-Sun; Poland, Michael; Miklius, Asta; Lu, Zhong

    2015-01-01

    Multiple-aperture SAR interferometry (MAI) has demonstrated outstanding measurement accuracy of along-track displacement when compared to pixel-offset-tracking methods; however, measuring slow-moving (cm/year) surface displacement remains a challenge. Stacking of multi-temporal observations is a potential approach to reducing noise and increasing measurement accuracy, but it is difficult to achieve a significant improvement by applying traditional stacking methods to multi-temporal MAI interferograms. This paper proposes an efficient MAI stacking method, where multi-temporal forward- and backward-looking residual interferograms are individually stacked before the MAI interferogram is generated. We tested the performance of this method using ENVISAT data from Kīlauea Volcano, Hawai‘i, where displacement on the order of several centimeters per year is common. By comparing results from the proposed stacking methods with displacements from GPS data, we documented measurement accuracies of about 1.03 and 1.07 cm/year for the descending and ascending tracks, respectively—an improvement of about a factor of two when compared with that from the conventional stacking approach. Three-dimensional surface-displacement maps can be constructed by combining stacked InSAR and MAI observations, which will contribute to a better understanding of a variety of geological phenomena.

  16. The LD loop as an important structural element required for transmission of the allosteric signal in the HtrA (DegP) protease from Escherichia coli.

    PubMed

    Figaj, Donata; Gieldon, Artur; Bartczak, Marlena; Koper, Tomasz; Zarzecka, Urszula; Lesner, Adam; Lipinska, Barbara; Skorko-Glonek, Joanna

    2016-09-01

    High-temperature requirement A (HtrA; DegP) from Escherichia coli, an important element of the extracytoplasmic protein quality-control system, is a member of the evolutionarily conserved family of serine proteases. The characteristic feature of this protein is its allosteric mode of activation. The regulatory loops, L3, L2, L1 and LD, play a crucial role in the transmission of the allosteric signal. Yet, the role of LD has not been fully elucidated. Therefore, we undertook a study to explain the role of the individual LD residues in inducing and maintaining the proteolytic activity of HtrA. We investigated the influence of amino acid substitutions located within the LD loop on the kinetics of a model substrate cleavage as well as on the dynamics of the oligomeric structure of HtrA. We found that the mutations that were expected to disturb the loop's structure and/or interactions with the remaining regulatory loops severely diminished the proteolytic activity of HtrA. The opposite effect, that is, increased activity, was observed for G174S substitution, which was predicted to strengthen the interactions mediated by LD. HtrAG174S protein had an equilibrium shifted toward the active enzyme and formed preferentially high-order oligomeric forms.

  17. Access to antiretroviral therapy among HIV/AIDS patients in Chiang Mai province, Thailand

    PubMed Central

    Himakalasa, Woraluck; Grisurapong, Siriwan; Phuangsaichai, Sasipen

    2013-01-01

    The objective of this study is to investigate the access to antiretroviral treatment among human immunodeficiency virus/acquired immunodeficiency syndrome (HIV/AIDS) patients in Chiang Mai province, Thailand. Access to antiretroviral treatment is defined in terms of availability, affordability, and acceptability. The data for the study were collected during the period of April 1, 2012–May 31, 2012 from a sample of 380 HIV/AIDS patients in eight hospitals who had received antiretroviral treatment for more than 6 months at the time of data collection. The results of the study show that for most patients, the average traveling time to access health care was acceptable, but the nearly half day waiting time caused them to be absent from their work. In particular, it took longer for patients in the rural and lower income groups to access the treatment than the other groups. Their travel times and food costs relating to the treatment were found to be relatively high and therefore these patients had a higher tendency to borrow or seek financial assistance from their relatives. However, due to improvements in the access to treatment, most patients were satisfied with the services they received. The results imply that policy should be implemented to raise the potential of subdistrict hospitals where access to antiretroviral treatment is available, with participating HIV/AIDS patients acting as volunteers in providing services and other forms of health promotion to new patients. Privacy issues could be reduced if the antiretroviral treatment was isolated from other health services. Additionally, efforts to educate HIV/AIDS patients and society at large should be made. PMID:23986652

  18. High Sensitivity Analysis of Nanoliter Volumes of Volatile and Nonvolatile Compounds using Matrix Assisted Ionization (MAI) Mass Spectrometry

    NASA Astrophysics Data System (ADS)

    Hoang, Khoa; Pophristic, Milan; Horan, Andrew J.; Johnston, Murray V.; McEwen, Charles N.

    2016-10-01

    First results are reported using a simple, fast, and reproducible matrix-assisted ionization (MAI) sample introduction method that provides substantial improvements relative to previously published MAI methods. The sensitivity of the new MAI methods, which requires no laser, high voltage, or nebulizing gas, is comparable to those reported for MALDI-TOF and n-ESI. High resolution full acquisition mass spectra having low chemical background are acquired from low nanoliters of solution using only a few femtomoles of analyte. The limit-of-detection for angiotensin II is less than 50 amol on an Orbitrap Exactive mass spectrometer. Analysis of peptides, including a bovine serum albumin digest, and drugs, including drugs in urine without a purification step, are reported using a 1 μL zero dead volume syringe in which only the analyte solution wetting the walls of the syringe needle is used in the analysis.

  19. RAB GTPASES ASSOCIATE WITH ISOLATED LIPID DROPLETS (LDS) AND SHOW ALTERED CONTENT AFTER ETHANOL ADMINISTRATION: POTENTIAL ROLE IN ALCOHOL-IMPAIRED LD METABOLISM

    PubMed Central

    Rasineni, Karuna; McVicker, Benita L.; Tuma, Dean J.; McNiven, Mark A.; Casey, Carol A.

    2013-01-01

    Background Alcoholic liver disease is manifested by the presence of fatty liver, primarily due to accumulation of hepatocellular lipid droplets (LDs). The presence of membrane-trafficking proteins (e.g. Rab GTPases) with LDs indicates that LDs may be involved in trafficking pathways known to be altered in ethanol damaged hepatocytes. Since these Rab GTPases are crucial regulators of protein trafficking, we examined the effect ethanol administration has on hepatic Rab protein content and association with LDs. Methods Male Wistar rats were pair-fed Lieber-DeCarli diets for 5 to 8 weeks. Whole liver and isolated LD fractions were analyzed. Identification of LDs and associated Rab proteins was performed in frozen liver or paraffin-embedded sections followed by immunohistochemical analysis. Results Lipid accumulation was characterized by larger LD vacuoles and increased total triglyceride content in ethanol-fed rats. Rabs 1, 2, 3d, 5, 7 and 18 were analyzed in post-nuclear supernatant (PNS) as well as LDs. All of the Rabs were found in the PNS, and Rabs 1, 2, 5 and 7 did not show alcohol-altered content, while Rab 3d content was reduced by over 80%, and Rab 18 also showed ethanol-induced reduction in content. Rab 3d was not found to associate with LDs, while all other Rabs were found in the LD fractions, and several showed an ethanol-related decrease (Rabs 2, 5, 7, 18). Immunohistochemical analysis revealed the enhanced content of a LD-associated protein, perilipin 2 (PLIN2) that was paralleled with an associated decrease of Rab 18 in ethanol-fed rat sections. Conclusion Chronic ethanol feeding was associated with increased PLIN2 and altered Rab GTPase content in enriched LD fractions. Although mechanisms driving these changes are not established, further studies on intracellular protein trafficking and LD biology after alcohol administration will likely contribute to our understanding of fatty liver disease. PMID:24117505

  20. Dental caries and gingivitis among pregnant and non-pregnant women in Chiang Mai, Thailand.

    PubMed

    Rakchanok, Noochpoung; Amporn, Dejpitak; Yoshida, Yoshitoku; Harun-Or-Rashid, Md; Sakamoto, Junichi

    2010-02-01

    The aims of this study were to identify dental caries and gingivitis among pregnant women, and to compare it with those in non-pregnant women in Chiang Mai, Thailand. Data were collected from 197 women (94 pregnant and 103 non-pregnant) from June to August, 2008. Dental caries and gingivitis was defined clinically according to the World Health Organization (WHO) diagnostic criteria. Over 74.0% of pregnant women had caries, and 86.2% had gingivitis. There were significant differences between pregnant and non-pregnant women with regard to dental caries (p < 0.001) and gingivitis (p = 0.021). The pregnant women were 2.9 times more likely to suffer from dental caries (95% confidence intervals (CI), 1.6-5.4), and 2.2 times more (95% CI, 1.1-4.7) from gingivitis compared to non-pregnant women. Farmers (Odd ratio (OR), 7.0; 95% CI, 1.8-26.3), high school graduation (OR, 3.0; 95% CI, 1.2-7.3), and universal health insurance coverage (OR, 2.1; 95% CI, 1.0-4.3) were significant predictors for gingivitis. Only high school graduates were found to be significant predictors of dental caries with an OR of 2.8 (95% CI, 1.2-6.3). Poor oral hygiene (OR, 2.2; 95% CI, 0.8-6.5), lack of knowledge (OR, 2.0; 95% CI, 0.6-6.3), and poor oral hygiene habits (OR, 3.0; 95% CI, 1.1-8.6) were important risk factors for dental caries. Similarly, inadequate oral hygiene status (OR, 24.8; 95% CI, 5.5-112.2), and poor oral health habits (OR, 5.2; 95% CI, 1.1-25.2) were found to be significant risk factors for gingivitis among pregnant women indicating, that most women should be trained in proper oral hygiene practices. Community awareness programs should be conducted to increase women's awareness of such hygienic practices.

  1. Application of an extension of the MAI method to the Acapulco City, Mexico

    NASA Astrophysics Data System (ADS)

    Contreras, M.; Aguirre, J.

    2001-12-01

    The site effects and the source parameters, are inverted from a Fourier displacement spectra of seismograms that are corrected by geometrical spreading and regional attenuation valid for south center of Mexico(Ordaz and Singh, 1992). We used Genetic Algorithms (GA) to perform the non-linear inversion, like in the MAI method (Moya et al., 2000) . The GA have proved to produce better results than other traditional methods which are frequently trapped in a local minimum. GA is a method that mimics the evolution laws in living creatures. The best individuals reproduce and develop themselves with every generation. In our case each individual correspond to one source and the genes correspond to the source parameters. As in nature, the best source remain and are improved with each iteration. We assume that the site effect at each station are the same independently of the earthquake, because of that we can search for the combination of sources that can produce the smaller standard deviation of the estimated site effects from the different Fourier displacement earthquake spectra. Then we use the obtained site effects to generate a Fourier displacement spectra of an earthquake scenario. With this, we are able to compute the response spectra by means of random vibration theory (Reinoso et al., 1990). We apply this method to four stations located in the Acapulco City, Mexico, that recorded four earthquakes with epicenter located in the Guerrero Subduction Zone. The site effect estimated for one of the stations, called ACAZ, shows a good agreement with the estimated by Chávez-García et al. (1994) using spectral ratios between the ACAZ station and a rock reference site. Also we compare the response spectra from other earthquake, obtained by the former method and the response spectra computed using the acceleration record. We find an acceptable correlation between them. Chávez-García, J. Cuenca y M. Cárdenas (1994), "Estudio complementario de efectos de sitio en Acapulco

  2. Effects of temperature on mortality in Chiang Mai city, Thailand: a time series study

    PubMed Central

    2012-01-01

    Background The association between temperature and mortality has been examined mainly in North America and Europe. However, less evidence is available in developing countries, especially in Thailand. In this study, we examined the relationship between temperature and mortality in Chiang Mai city, Thailand, during 1999–2008. Method A time series model was used to examine the effects of temperature on cause-specific mortality (non-external, cardiopulmonary, cardiovascular, and respiratory) and age-specific non-external mortality (<=64, 65–74, 75–84, and > =85 years), while controlling for relative humidity, air pollution, day of the week, season and long-term trend. We used a distributed lag non-linear model to examine the delayed effects of temperature on mortality up to 21 days. Results We found non-linear effects of temperature on all mortality types and age groups. Both hot and cold temperatures resulted in immediate increase in all mortality types and age groups. Generally, the hot effects on all mortality types and age groups were short-term, while the cold effects lasted longer. The relative risk of non-external mortality associated with cold temperature (19.35°C, 1st percentile of temperature) relative to 24.7°C (25th percentile of temperature) was 1.29 (95% confidence interval (CI): 1.16, 1.44) for lags 0–21. The relative risk of non-external mortality associated with high temperature (31.7°C, 99th percentile of temperature) relative to 28°C (75th percentile of temperature) was 1.11 (95% CI: 1.00, 1.24) for lags 0–21. Conclusion This study indicates that exposure to both hot and cold temperatures were related to increased mortality. Both cold and hot effects occurred immediately but cold effects lasted longer than hot effects. This study provides useful data for policy makers to better prepare local responses to manage the impact of hot and cold temperatures on population health. PMID:22613086

  3. RuBPCase activase mediates growth-defense tradeoffs: Silencing RCA redirects JA flux from JA-Ile to MeJA to attenuate induced defense responses in Nicotiana attenuata

    PubMed Central

    Mitra, Sirsha; Baldwin, Ian T.

    2014-01-01

    Summary RuBPCase activase (RCA), an abundant photosynthetic protein is strongly down-regulated in response to Manduca sexta’s oral secretion (OS) in Nicotiana attenuata. RCA-silenced plants are impaired not only in photosynthetic capacity and growth, but also in jasmonic acid (JA)-isoleucine (Ile) signaling, and herbivore resistance mediated by JA-Ile dependent defense traits. These responses are consistent with a resource-based growth-defense trade-off. Since JA+Ile-supplementation of OS restored WT levels of JA-Ile, defenses and resistance to M. sexta, but OS supplemented individually with JA- or Ile did not, the JA-Ile deficiency of RCA-silenced plants could not be attributed to lower JA or Ile pools or JAR4/6 conjugating activity. Similar levels of JA-Ile derivatives after OS elicitation indicated unaltered JA-Ile turnover and lower levels of other JA-conjugates ruled out competition from other conjugation reactions. RCA-silenced plants accumulated more methyl jasmonate (MeJA) after OS elicitation, which corresponded with increased jasmonate methyltransferase (JMT) activity. RCA-silencing phenocopies JMT over-expression, wherein elevated JMT activity redirects OS-elicited JA flux towards inactive MeJA, creating a JA sink which depletes JA-Ile and its associated defense responses. Hence RCA plays an additional non-photosynthetic role in attenuating JA-mediated defenses and their associated costs potentially allowing plants to anticipate resource-based constraints on growth before they actually occur. PMID:24491116

  4. Genetic Diversity of Norovirus and Sapovirus in Hospitalized Infants with Sporadic Cases of Acute Gastroenteritis in Chiang Mai, Thailand

    PubMed Central

    Hansman, Grant S.; Katayama, Kazuhiko; Maneekarn, Niwat; Peerakome, Supatra; Khamrin, Pattara; Tonusin, Supin; Okitsu, Shoko; Nishio, Osamu; Takeda, Naokazu; Ushijima, Hiroshi

    2004-01-01

    Stool specimens from hospitalized infants with sporadic gastroenteritis in Chiang Mai, Thailand, between July 2000 and July 2001 were examined for norovirus and sapovirus by reverse transcription-PCR and sequence analysis. These viruses were identified in 13 of 105 (12%) specimens. One strain was found to be a recombinant norovirus. PMID:15004104

  5. MAI (Multi-Dimensional Activity Based Integrated Approach): A Strategy for Cognitive Development of the Learners at the Elementary Stage

    ERIC Educational Resources Information Center

    Basantia, Tapan Kumar; Panda, B. N.; Sahoo, Dukhabandhu

    2012-01-01

    Cognitive development of the learners is the prime task of each and every stage of our school education and its importance especially in elementary state is quite worth mentioning. Present study investigated the effectiveness of a new and innovative strategy (i.e., MAI (multi-dimensional activity based integrated approach)) for the development of…

  6. 17 CFR 240.15Ba1-5 - Amendments to Form MA and Form MA-I.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 17 Commodity and Securities Exchanges 4 2014-04-01 2014-04-01 false Amendments to Form MA and Form MA-I. 240.15Ba1-5 Section 240.15Ba1-5 Commodity and Securities Exchanges SECURITIES AND EXCHANGE COMMISSION (CONTINUED) GENERAL RULES AND REGULATIONS, SECURITIES EXCHANGE ACT OF 1934 Rules and...

  7. 17 CFR 249.1310 - Form MA-I, for providing information regarding natural person municipal advisors, and for...

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ..., SECURITIES EXCHANGE ACT OF 1934 Forms for Registration of Municipal Advisors and for Providing Information... 17 Commodity and Securities Exchanges 4 2014-04-01 2014-04-01 false Form MA-I, for providing information regarding natural person municipal advisors, and for amendments to such information....

  8. Emerging toxicity of 5,6-methylenedioxy-2-aminoindane (MDAI): Pharmacokinetics, behaviour, thermoregulation and LD50 in rats.

    PubMed

    Páleníček, Tomáš; Lhotková, Eva; Žídková, Monika; Balíková, Marie; Kuchař, Martin; Himl, Michal; Mikšátková, Petra; Čegan, Martin; Valeš, Karel; Tylš, Filip; Horsley, Rachel R

    2016-08-01

    MDAI (5,6-Methylenedioxy-2-aminoindane) has a reputation as a non-neurotoxic ecstasy replacement amongst recreational users, however the drug has been implicated in some severe and lethal intoxications. Due to this, and the fact that the drug is almost unexplored scientifically we investigated a broad range of effects of acute MDAI administration: pharmacokinetics (in sera, brain, liver and lung); behaviour (open field; prepulse inhibition, PPI); acute effects on thermoregulation (in group-/individually-housed rats); and systemic toxicity (median lethal dose, LD50) in Wistar rats. Pharmacokinetics of MDAI was rapid, maximum median concentration in serum and brain was attained 30min and almost returned to zero 6h after subcutaneous (sc.) administration of 10mg/kg MDAI; brain/serum ratio was ~4. MDAI particularly accumulated in lung tissue. In the open field, MDAI (5, 10, 20 and 40mg/kg sc.) increased exploratory activity, induced signs of behavioural serotonin syndrome and reduced locomotor habituation, although by 60min some effects had diminished. All doses of MDAI significantly disrupted PPI and the effect was present during the onset of its action as well as 60min after treatment. Unexpectedly, 40mg/kg MDAI killed 90% of animals in the first behavioural test, hence LD50 tests were conducted which yielded 28.33mg/kg sc. and 35mg/kg intravenous but was not established up to 40mg/kg after gastric administration. Disseminated intravascular coagulopathy (DIC) with brain oedema was concluded as a direct cause of death in sc. treated animals. Finally, MDAI (10, 20mg/kg sc.) caused hyperthermia and perspiration in group-housed rats. In conclusion, the drug had fast pharmacokinetics and accumulated in lipohilic tissues. Behavioural findings were consistent with mild, transient stimulation with anxiolysis and disruption of sensorimotor processing. Together with hyperthermia, the drug had a similar profile to related entactogens, especially 3

  9. Insecticidal activity of a Moroccan strain of Streptomyces phaeochromogenes LD-37 on larvae, pupae and adults of the Mediterranean fruit fly, Ceratitis capitata (Diptera: Tephritidae).

    PubMed

    Samri, S E; Baz, M; Ghalbane, I; El Messoussi, S; Zitouni, A; El Meziane, A; Barakate, M

    2017-04-01

    The Mediterranean fruit fly (medfly), Ceratitis capitata, is considered the most important fruit pest worldwide. Its management is mainly based on the use of chemical insecticides. Although these conventional pesticides are effective at high doses, they cause considerable human health and environment problems. Thus, the aim of this study was to assess insecticidal activity of Moroccan actinobacteria against C. capitata. A total of 12 preselected actinobacteria isolated from various Moroccan habitats were screened for their insecticidal activity against larvae, pupae and adults of C. capitata. Four actinobacteria isolates were significantly active against the first-instar larvae, and nine were active against the medfly adult, while no significant mortality was obtained against the third-instar larval and pupal stages. Among the selected isolates, the biological screening revealed that strain Streptomyces LD-37, which showed 99.4% similarity with Streptomyces phaeochromogenes, exhibited the maximal corrected larval mortality of 98%. Moreover, the isolates AS1 and LD-37 showed the maximum significant corrected mortality against adults of 32.5 and 28.2%, respectively. The crude extract obtained from a fermented culture of strain S. phaeochromogenes LD-37 was separated into six fractions by thin layer chromatography. Fractions F3 and F4 caused a significant corrected larval mortality of 66.7 and 53.3%, respectively; whereas the maximum reduction in adult emergence was obtained with fraction F4. This finding could be useful for utilizing S. phaeochromogenes LD-37 as an alternative to chemical insecticides in pest management of C. capitata.

  10. Direct LD-FTMS Detection of Mineral-Associated PAHs and Their Influence on the Detection of Co-Existing Amino Acids

    SciTech Connect

    Beizhan Yan; Daphne L. Stoner; Jill R. Scott

    2007-04-01

    Polycyclic aromatic hydrocarbon (PAH) compounds and amino acids (AAs) are both ubiquitous throughout the universe and can be co-located in mineral matrices (e.g., meteorites); therefore, co-detection of PAHs and AAs associated with terrestrial and extra-terrestrial minerals is of interest. Nine PAH compounds representing four chemical classes of PAH (unsubstituted, acetyl-, amino-, and nitro-substituted) were applied onto the surface of quartz, plagioclase, olivine, and ilmenite mineral standards and analyzed using laser desorption/ionization Fourier transform mass spectrometry (LD-FTMS). Mass-to-charge peaks derived from PAH compounds were detected from the surfaces of all minerals evaluated. All PAH compounds were detected in the positive ion mode, whereas only nitro-substituted PAH compounds were detected in negative ion mode. In this and earlier studies, the ability to directly detect mineral-associated AAs by LD-FTMS was dependent on the mineral geomatrix. On iron-bearing minerals AAs appeared as highly fragmented ions in the spectra or were not detectable; however, the addition of the PAH chrysene enabled the ionization and detection of AAs threonine and histidine by LD-FTMS. Thus, for mineral systems such as meteorites, interstellar dust particles, soils, and sediments, the co-detection of AAs associated with PAHs by LD-FTMS is feasible.

  11. Mediating Factors of a School-Based Multi-Component Smoking Prevention Intervention: The LdP Cluster Randomized Controlled Trial

    ERIC Educational Resources Information Center

    Carreras, G.; Bosi, S.; Angelini, P.; Gorini, G.

    2016-01-01

    The aim of this study was to investigate factors mediating the effects of Luoghi di Prevenzione (LdP) smoking prevention intervention based on social competence and social influence approaches, and characterized by peer-led school-based interventions, out-of-school workshops, school lessons, and by enforcing the school anti-smoking policy.…

  12. A new highly adaptable design of shear-flow device for orientation of macromolecules for Linear Dichroism (LD) measurement.

    PubMed

    Lundahl, P Johan; Kitts, Catherine C; Nordén, Bengt

    2011-08-21

    This article presents a new design of flow-orientation device for the study of bio-macromolecules, including DNA and protein complexes, as well as aggregates such as amyloid fibrils and liposome membranes, using Linear Dichroism (LD) spectroscopy. The design provides a number of technical advantages that should make the device inexpensive to manufacture, easier to use and more reliable than existing techniques. The degree of orientation achieved is of the same order of magnitude as that of the commonly used concentric cylinders Couette flow cell, however, since the device exploits a set of flat strain-free quartz plates, a number of problems associated with refraction and birefringence of light are eliminated, increasing the sensitivity and accuracy of measurement. The device provides similar shear rates to those of the Couette cell but is superior in that the shear rate is constant across the gap. Other major advantages of the design is the possibility to change parts and vary sample volume and path length easily and at a low cost.

  13. Aerothermodynamic performance and thermal protection design for blunt re-entry bodies at L/D = 0.3

    NASA Technical Reports Server (NTRS)

    Caram, Jose M.; Kowal, T. J.

    1993-01-01

    Aerodynamic heating and thermal protection design analyses were performed for three blunt re-entry bodies at an L/D = 0.3 returning from low earth orbit. These configurations consisted of a scaled up Apollo command module, a Viking re-entry vehicle, and an Aeroassist Flight Experiment (AFE) aerobrake, each with a maximum diameter of 4.42 m. The aerothermodynamic analysis determined the equilibrium stagnation point heating rate and heat load for nominal and 3-sigma re-entry trajectories and the distribution of heating along the pitch and yaw planes for each of the vehicles at the time of highest heat flux. Using the predicted heating rates and heating distributions, a Thermal Protection System (TPS) design with flight certified materials was tailored for each of the configurations. Results indicated that the heating to the corner of the Viking aeroshell would exceed current limits of reusable tile material. Also, the maximum heating for the AFE would be 15 percent greater than the maximum heating for the Apollo flying the same trajectory. TPS designs showed no significant advantage in TPS weight between the different vehicles; however, heat-shield areal density comparisons showed the Apollo configuration to be the most efficient in terms of TPS weight.

  14. Monomethylarsonous acid (MMA(III)) and arsenite: LD(50) in hamsters and in vitro inhibition of pyruvate dehydrogenase.

    PubMed

    Petrick, J S; Jagadish, B; Mash, E A; Aposhian, H V

    2001-06-01

    Monomethylarsonous acid (MMA(III)), a metabolite of inorganic arsenic, has received very little attention from investigators of arsenic metabolism in humans. MMA(III), like sodium arsenite, contains arsenic in the +3 oxidation state. Although we have previously demonstrated that it is more toxic than arsenite in cultured Chang human hepatocytes, there are no data showing in vivo toxicity of MMA(III). When MMA(III) or sodium arsenite was administered intraperitoneally to hamsters, the LD(50)s were 29.3 and 112.0 micromol/kg of body wt, respectively. In addition, inhibition of hamster kidney or purified porcine heart pyruvate dehydrogenase (PDH) activity by MMA(III) or arsenite was determined. To inhibit hamster kidney PDH activity by 50%, the concentrations (mean +/- SE) of MMA(III) as methylarsine oxide, MMA(III) as diiodomethylarsine, and arsenite were 59.9 +/- 6.5, 62.0 +/- 1.8, and 115.7 +/- 2.3 microM, respectively. To inhibit activity of purified porcine heart PDH activity by 50%, the concentrations (mean +/- SE) of MMA(III) as methylarsine oxide and arsenite were 17.6 +/- 4.1 and 106.1 +/- 19.8 microM, respectively. These data demonstrate that MMA(III) is more toxic than inorganic arsenite, both in vivo and in vitro, and call into question the hypothesis that methylation of inorganic arsenic is a detoxication process.

  15. Effect of water/fuel emulsions and a cerium-based combustion improver additive on HD and LD diesel exhaust emissions.

    PubMed

    Farfaletti, Arianna; Astorga, Covadonga; Martini, Giorgio; Manfredi, Urbano; Mueller, Anne; Rey, Maria; De Santi, Giovanni; Krasenbrink, Alois; Larsen, Bo R

    2005-09-01

    One of the major technological challenges for the transport sector is to cut emissions of particulate matter (PM) and nitrogen oxides (NOx) simultaneously from diesel vehicles to meet future emission standards and to reduce their contribution to the pollution of ambient air. Installation of particle filters in all existing diesel vehicles (for new vehicles, the feasibility is proven) is an efficient but expensive and complicated solution; thus other short-term alternatives have been proposed. It is well known that water/diesel (W/ D) emulsions with up to 20% water can reduce PM and NOx emissions in heavy-duty (HD) engines. The amount of water that can be used in emulsions for the technically more susceptible light-duty (LD) vehicles is much lower, due to risks of impairing engine performance and durability. The present study investigates the potential emission reductions of an experimental 6% W/D emulsion with EURO-3 LD diesel vehicles in comparison to a commercial 12% W/D emulsion with a EURO-3 HD engine and to a Cerium-based combustion improver additive. For PM, the emulsions reduced the emissions with -32% for LD vehicles (mass/km) and -59% for the HD engine (mass/ kWh). However, NOx emissions remained unchanged, and emissions of other pollutants were actually increased forthe LD vehicles with +26% for hydrocarbons (HC), +18% for CO, and +25% for PM-associated benzo[a]pyrene toxicity equivalents (TEQ). In contrast, CO (-32%), TEQ (-14%), and NOx (-6%) were reduced by the emulsion for the HD engine, and only hydrocarbons were slightly increased (+16%). Whereas the Cerium-based additive was inefficient in the HD engine for all emissions except for TEQ (-39%), it markedly reduced all emissions for the LD vehicles (PM -13%, CO -18%, HC -26%, TEQ -25%) except for NOx, which remained unchanged. The presented data indicate a strong potential for reductions in PM emissions from current diesel engines by optimizing the fuel composition.

  16. Lead and cadmium in leaves of deciduous trees in Beijing, China: development of a metal accumulation index (MAI).

    PubMed

    Liu, Yan-Ju; Zhu, Yong-Guan; Ding, Hui

    2007-01-01

    Lead and cadmium uptake was investigated for common deciduous street trees in Beijing in this study. Species having Cd accumulation included Populus tomentosa, Sophora japonica and Catalpa speciosa. P. tomentosa had the highest ratios between leaf and soil Cd (0.848), followed by S. japonica (0.536), C. speciosa (0.493), Paulownia tomentosa (0.453) and Juglans regia (0.415). Pb levels were high in leaves of C. speciosa, J. regia and Pa. tomentosa. S. japonica had the highest ratio between leaf Pb and soil Pb (0.146), followed by Pa. tomentosa (0.143), Ginko biloba (0.103) and C. speciosa (0.095). A predictive foliar metal accumulation index (MAI) was developed and C. speciosa was calculated to have the highest MAI value (53.8). This suggests that C. speciosa would be a good choice for planting in areas of Beijing where soil contamination with Cd and Pb may be a problem.

  17. LD pumped high-repetition-rate high-power 532nm Nd:YAG/LBO solid state laser

    NASA Astrophysics Data System (ADS)

    Li, Pingxue; Liu, Dongyu; Chi, Junjie; Yang, Chun; Zhao, Ziqiang; Hu, Haowei; Zhang, Guangju; Yao, Yifei

    2013-09-01

    Diode pumped solid state 532 nm green laser is widely required for many industrial, medical and scientific applications. Among most of these applications, high power quasi-continuous-wave (QCW) green laser output is demanded. This can be efficiently achieved through a diode-side-pumped acoustic-optic Q-switched Nd:YAG laser with an intracavity second harmonic generation (SHG). In our experiment, LBO crystal is used for the second harmonic generation of high-average-power lasers of near infrared (NIR) range, though its effective NLO coefficient deff is relatively small. It is because of its high damage threshold (greater than 2.5 GW/cm2), large acceptance angle, small walk-off angle, and the nonhygroscopic characteristic. In this paper, we reported a high-repetition-rate high-power diode-side-pumped AO Q-switched Nd:YAG 532 nm laser. A plane-plane cavity with two rods, two AO Q-switches and the type II critical phase-matched LBO at room temperature were employed. Under the LD pump power of 480 W, 95.86 W at 1064 nm wavelength was achieved when the repetition rate was 15 kHz, and the 532 nm average output power of 44.77 W was obtained, with a pulse width of 111.7 ns, corresponding to an optical to optical conversion efficiency of 46.7% from 1064 nm to 532 nm. The 532 nm average output power was 40.10 W at a repetition rate of 10 kHz with a pulse width of 78.65 ns. The output characteristics of the SHG varying with the pumping current and the pulse repetition frequency (PRF) of the laser were also investigated. Further improvement of the SHG is under study.

  18. The Mais Médicos (More Doctors) Program, the infrastructure of Primary Health Units and the Municipal Human Development Index.

    PubMed

    Soares, Joaquim José; Machado, Maria Helena; Alves, Cecília Brito

    2016-09-01

    The main objective of this article was to examine the context in which professionals working within the Mais Médicos (More Doctors) Program operate. This study used the infrastructure scale of primary health units (PHUs), which was recently developed by Soares Neto and colleagues to provide more information regarding the relationship between the infrastructure of PHUs and the Municipal Human Development Index (MHDI) of municipalities that received Mais Médicos Program doctors. Using exploratory and inferential statistics, the article shows that 65.2% of the PHUs that received Mais Médicos Program doctors had medium-quality infrastructure and only 5.8% of them had low-quality infrastructure. The correlation of 0.50 between the infrastructure indicator and the MHDI points to a moderate tendency for municipalities with low MHDIs to have more precarious PHUs. Using multiple linear regression analysis it can be inferred that the main factor that contributed to the increase in the infrastructure indicator of the PHUs was the average municipal income. On the other hand, the factor that negatively affected the infrastructure of the PHUs was being located in the north or northeast regions.

  19. The University of Jaén Astronomical Observatory

    NASA Astrophysics Data System (ADS)

    Martí, Josep; Luque-Escamilla, Pedro L.; García-Hernández, María T.

    2017-01-01

    We present a description and instrumental characterization of the photometric equipment of the Astronomical Observatory of the University of Jaén. The observatory hosts a 41 cm automated telescope inside a 4 m dome located at the university main campus, in the outskirts of the city of Jaén (Spain). This facility is used for educational, outreach and occasional scientific research on bright stellar objects. Despite the observatory location in a light polluted urban area, its performance for differential photometry studies has proven to be very acceptable. The discovery of the Be star LS I +5979 as a peculiar eclipsing binary system is so far the most relevant achievement.

  20. Measuring the potency labelling of onabotulinumtoxinA (Botox(®)) and incobotulinumtoxinA (Xeomin (®)) in an LD50 assay.

    PubMed

    Dressler, Dirk; Mander, Gerd; Fink, Klaus

    2012-01-01

    The biological potency of botulinum toxin (BT) drugs is determined by a standardised LD50 assay. However, the potency labelling varies vary amongst different BT drugs. One reason for this may be differences in the LD50 assays applied. When five unexpired batches of onabotulinumtoxinA (Botox(®)) and incobotulinumtoxinA (Xeomin(®)) are compared in the Xeomin(®) batch release assay, the potency variability of both BT drugs fell within the range allowed by the European Pharmacopoiea. Statistical analyses failed to detect differences in the potency labelling of both products. Although the existence of a conversion ratio has been questioned recently, our experimental data are in line with previous clinical experience showing that Botox(®) and Xeomin(®) can be compared using a 1:1 conversion ratio. Identical potency labelling allows easy exchange of both BT drugs in a therapeutic setting, and direct comparison of efficacy, adverse effects and costs.

  1. Functional receptor molecules CD300lf and CD300ld within the CD300 family enable murine noroviruses to infect cells

    PubMed Central

    Haga, Kei; Fujimoto, Akira; Takai-Todaka, Reiko; Miki, Motohiro; Doan, Yen Hai; Murakami, Kosuke; Yokoyama, Masaru; Murata, Kazuyoshi; Nakanishi, Akira; Katayama, Kazuhiko

    2016-01-01

    Norovirus is the leading cause of acute gastroenteritis worldwide. Since the discovery of human norovirus (HuNoV), an efficient and reproducible norovirus replication system has not been established in cultured cells. Although limited amounts of virus particles can be produced when the HuNoV genome is directly transfected into cells, the HuNoV cycle of infection has not been successfully reproduced in any currently available cell-culture system. Those results imply that the identification of a functional cell-surface receptor for norovirus might be the key to establishing a norovirus culture system. Using a genome-wide CRISPR/Cas9 guide RNA library, we identified murine CD300lf and CD300ld as functional receptors for murine norovirus (MNV). The treatment of susceptible cells with polyclonal antibody against CD300lf significantly reduced the production of viral progeny. Additionally, ectopic CD300lf expression in nonsusceptible cell lines derived from other animal species enabled MNV infection and progeny production, suggesting that CD300lf has potential for dictating MNV host tropism. Furthermore, CD300ld, which has an amino acid sequence in the N-terminal region of its extracellular domain that is highly homologous to that of CD300lf, also functions as a receptor for MNV. Our results indicate that direct interaction of MNV with two cell-surface molecules, CD300lf and CD300ld, dictates permissive noroviral infection. PMID:27681626

  2. Crystal Structure of Barley Limit Dextrinase-Limit Dextrinase Inhibitor (LD-LDI) Complex Reveals Insights into Mechanism and Diversity of Cereal Type Inhibitors*

    PubMed Central

    Møller, Marie S.; Vester-Christensen, Malene B.; Jensen, Johanne M.; Hachem, Maher Abou; Henriksen, Anette; Svensson, Birte

    2015-01-01

    Molecular details underlying regulation of starch mobilization in cereal seed endosperm remain unknown despite the paramount role of this process in plant growth. The structure of the complex between the starch debranching enzyme barley limit dextrinase (LD), hydrolyzing α-1,6-glucosidic linkages, and its endogenous inhibitor (LDI) was solved at 2.7 Å. The structure reveals an entirely new and unexpected binding mode of LDI as compared with previously solved complex structures of related cereal type family inhibitors (CTIs) bound to glycoside hydrolases but is structurally analogous to binding of dual specificity CTIs to proteases. Site-directed mutagenesis establishes that a hydrophobic cluster flanked by ionic interactions in the protein-protein interface is vital for the picomolar affinity of LDI to LD as assessed by analysis of binding by using surface plasmon resonance and also supported by LDI inhibition of the enzyme activity. A phylogenetic analysis identified four LDI-like proteins in cereals among the 45 sequences from monocot databases that could be classified as unique CTI sequences. The unprecedented binding mechanism shown here for LDI has likely evolved in cereals from a need for effective inhibition of debranching enzymes having characteristic open active site architecture. The findings give a mechanistic rationale for the potency of LD activity regulation and provide a molecular understanding of the debranching events associated with optimal starch mobilization and utilization during germination. This study unveils a hitherto not recognized structural basis for the features endowing diversity to CTIs. PMID:25792743

  3. Internal Structure and Development of Keyboard Skills in Spanish-Speaking Primary-School Children With and Without LD in Writing.

    PubMed

    Jiménez, Juan E; Marco, Isaac; Suárez, Natalia; González, Desirée

    2016-04-07

    This study had two purposes: examining the internal structure of theTest Estandarizado para la Evaluación Inicial de la Escritura con Teclado(TEVET; Spanish Keyboarding Writing Test), and analyzing the development of keyboarding skills in Spanish elementary school children with and without learning disabilities (LD) in writing. A group of 1,168 elementary school children carried out the following writing tasks: writing the alphabet in order from memory, allograph selection, word copying, writing dictated words with inconsistent spelling, writing pseudowords from dictation, and independent composition of sentence. For this purpose, exploratory factor analysis for the TEVET was conducted. Principal component analysis with a varimax rotation identified three factors with eigenvalues greater than 1.0. Based on factorial analysis, we analyzed the keyboarding skills across grades in Spanish elementary school children with and without LD (i.e., poor handwriters compared with poor spellers, who in turn were compared with mixed compared with typically achieving writers). The results indicated that poor handwriters did not differ from typically achieving writers in phonological processing, visual-orthographic processing, and sentence production components by keyboarding. The educational implications of the findings are analyzed with regard to acquisition of keyboarding skills in children with and without LD in transcription.

  4. Spatial Correlation Analysis between Particulate Matter 10 (PM10) Hazard and Respiratory Diseases in Chiang Mai Province, Thailand

    NASA Astrophysics Data System (ADS)

    Trang, N. Ha; Tripathi, N. K.

    2014-11-01

    Every year, during dry season, Chiang Mai and other northern provinces of Thailand face the problem of haze which is mainly generated by the burning of agricultural waste and forest fire, contained high percentage of particulate matter. Particulate matter 10 (PM10), being very small in size, can be inhaled easily to the deepest parts of the human lung and throat respiratory functions. Due to this, it increases the risk of respiratory diseases mainly in the case of continuous exposure to this seasonal smog. MODIS aerosol images (MOD04) have been used for four weeks in March 2007 for generating the hazard map by linking to in-situ values of PM10. Simple linear regression model between PM10 and AOD got fair correlation with R2 = 0.7 and was applied to transform PM10 pattern. The hazard maps showed the dominance of PM10 in northern part of Chiang Mai, especially in second week of March when PM10 level was three to four times higher than standard. The respiratory disease records and public health station of each village were collected from Provincial Public Health Department in Chiang Mai province. There are about 300 public health stations out of 2070 villages; hence thiessen polygon was created to determine the representative area of each public health station. Within each thiessen polygon, respiratory disease incident rate (RDIR) was calculated based on the number of patients and population. Global Moran's I was computed for RDIR to explore spatial pattern of diseases through four weeks of March. Moran's I index depicted a cluster pattern of respiratory diseases in 2nd week than other weeks. That made sense for a relationship between PM10 and respiratory diseases infections. In order to examine how PM10 affect the human respiratory system, geographically weighted regression model was used to observe local correlation coefficient between RDIR and PM10 across study area. The result captured a high correlation between respiratory diseases and high level of PM10 in

  5. YiQiFuMai Powder Injection Ameliorates Cerebral Ischemia by Inhibiting Endoplasmic Reticulum Stress-Mediated Neuronal Apoptosis

    PubMed Central

    Hu, Yang

    2016-01-01

    YiQiFuMai (YQFM) powder injection as a modern preparation derived from Sheng Mai San, a traditional Chinese medicine, has been widely used in the treatment of cardiovascular and cerebrovascular diseases. However, its neuroprotective effect and underlying mechanism in cerebral ischemia remain to be explored. The present study was designed to investigate the neuroprotective effect of YQFM on endoplasmic reticulum (ER) stress-mediated neuronal apoptosis in the permanent middle cerebral artery occlusion- (MCAO-) injured mice and the oxygen-glucose deprivation- (OGD-) induced pheochromocytoma (PC12) cells. The results showed that single administration of YQFM (1.342 g/kg, i.p.) could reduce the brain infarction and improve the neurological deficits and the cerebral blood flow (CBF) after MCAO for 24 h in mice. Moreover, incubation with YQFM (100, 200, and 400 μg/mL) could increase the cell viability, decrease the caspase-3 activity, and inhibit the cell apoptosis in OGD-induced PC12 cells for 12 h. In addition, YQFM treatment could significantly modulate cleaved caspase-3 and Bcl-2 expressions and inhibit the expressions of ER stress-related marker proteins and signaling pathways in vivo and in vitro. In conclusion, our findings provide the first evidence that YQFM ameliorates cerebral ischemic injury linked with modulating ER stress-related signaling pathways, which provided some new insights for its prevention and treatment of cerebral ischemia diseases. PMID:27087890

  6. Impact of the Mais Médicos (More Doctors) Program in reducing physician shortage in Brazilian Primary Healthcare.

    PubMed

    Girardi, Sábado Nicolau; Stralen, Ana Cristina de Sousa van; Cella, Joana Natalia; Wan Der Maas, Lucas; Carvalho, Cristiana Leite; Faria, Erick de Oliveira

    2016-09-01

    The Mais Médicos (More Doctors) Program (PMM) was put in place in Brazil aiming to reduce inequalities in access to Primary Healthcare. Based on diverse evidence that pointed to a scenario of profound shortage of doctors in the country, one of its central thrusts was emergency provision of these professionals in vulnerable areas, referred to as the Mais Médicos para o Brasil (More Doctors for Brazil) Project. The article analyses the impact of the PMM in reducing shortage of physicians in Brazilian municipalities. To do this, it uses the Primary Healthcare Physicians Shortage Index, which identifies and measures the shortage in the periods of March 2003 and September 2015, before and after implementation of the program. The results show that there was a substantial increase in the supply of physicians in primary healthcare in the period, which helped reduce the number of municipalities with shortage from 1,200 to 777. This impact also helped reduce inequalities between municipalities, but the inequities in distribution persisted. It was also found that there was a reduction in the regular supply of doctors made by municipalities, suggesting that these were being simply substituted by the supply coming from the program. Thus, an overall situation of insecurity in care persists, reflecting the dependence of municipalities on the physician supply from the federal government.

  7. Increased SA in NPR1-silenced plants antagonizes JA and JA-dependent direct and indirect defenses in herbivore-attacked Nicotiana attenuata in nature.

    PubMed

    Rayapuram, Cbgowda; Baldwin, Ian T

    2007-11-01

    The phytohormone jasmonic acid (JA) is known to mediate herbivore resistance, while salicylic acid (SA) and non-expressor of PR-1 (NPR1) mediate pathogen resistance in many plants. Herbivore attack on Nicotiana attenuata elicits increases in JA and JA-mediated defenses, but also increases SA levels and Na-NPR1 transcripts from the plant's single genomic copy. SA treatment of wild-type plants increases Na-NPR1 and Na-PR1 transcripts. Plants silenced in NPR1 accumulation by RNAi (ir-npr1) are highly susceptible to herbivore and pathogen attack when planted in their native habitat in Utah. They are also impaired in their ability to attract Geocorus pallens predators, due to their decreased ability to release cis-alpha-bergamotene, a JA-elicited volatile 'alarm call'. In the glasshouse, Spodoptera exigua larvae grew better on ir-npr1 plants, which had low levels of JA, JA-isoleucine/leucine, lipoxygenase-3 (LOX3) transcripts and JA-elicited direct defense metabolites (nicotine, caffeoyl putrescine and rutin), but high levels of SA and isochorismate synthase (ICS) transcripts, suggesting de novo biosynthesis of SA. A microarray analysis revealed downregulation of many JA-elicited genes and upregulation of SA biosynthetic genes. JA treatment restored nicotine levels and resistance to S. exigua in ir-npr1 plants. We conclude that, during herbivore attack, NPR1 negatively regulates SA production, allowing the unfettered elicitation of JA-mediated defenses; when NPR1 is silenced, the elicited increases in SA production antagonize JA and JA-related defenses, making the plants susceptible to herbivores.

  8. The Complete Nucleotide Sequence of the Carbapenem Resistance-Conferring Conjugative Plasmid pLD209 from a Pseudomonas putida Clinical Strain Reveals a Chimeric Design Formed by Modules Derived from Both Environmental and Clinical Bacteria

    PubMed Central

    Marchiaro, Patricia M.; Brambilla, Luciano; Morán-Barrio, Jorgelina; Revale, Santiago; Pasteran, Fernando; Vila, Alejandro J.; Viale, Alejandro M.

    2014-01-01

    The complete sequence of the carbapenem-resistance-conferring conjugative plasmid pLD209 from a Pseudomonas putida clinical strain is presented. pLD209 is formed by 3 well-defined regions: an adaptability module encompassing a Tn402-like class 1 integron of clinical origin containing blaVIM-2 and aacA4 gene cassettes, partitioning and transfer modules, and a replication module derived from plasmids of environmental bacteria. pLD209 is thus a mosaic of modules originating in both the clinical and environmental (nonclinical) microbiota. PMID:24395220

  9. A new forest-dwelling Bent-toed Gecko (Squamata: Gekkonidae: Cyrtodactylus) from Doi Inthanon, Chiang Mai Province, northern Thailand.

    PubMed

    Kunya, Kirati; Sumontha, Montri; Panitvong, Nonn; Dongkumfu, Wuttipong; Sirisamphan, Thana; Pauwels, Olivier S G

    2015-01-14

    We describe a new forest-dwelling Cyrtodactylus from Doi Inthanon, Chiang Mai Province, northern Thailand. Cyrtodactylus inthanon sp. nov. is characterized by a maximum known SVL of 87.3 mm; 18 to 20 longitudinal rows of dorsal tubercles; a continuous series of 34 to 37 enlarged femoro-precloacal scales, including four to six pitted (female) or pore-bearing (male) scales on each femur separated by a diastema from five pitted (females) or pore-bearing (male) precloacal scales; no precloacal groove or depression; transversely enlarged subcaudal scales; and three to five irregular beige dorsal bands between limb insertions. The discovery of a new reptile endemic to Doi Inthanon reinforces the high importance of this mountain in terms of biodiversity conservation.

  10. Pharmacodynamics and potential synergistic effects of Mai-Luo-Ning injection on cardiovascular protection, based on molecular docking.

    PubMed

    Wu, Liang; Shen, Han-Yuan; Wu, Yu-Zheng; Yu, Xiao-Yi; Wang, Hong; Cheng, Xue-Fang; Wang, Guang-Ji; Hao, Hai-Ping

    2015-11-01

    As a computer-assisted approach, molecular docking has been universally applied in drug research and development and plays an important role in the investigation and evaluation of herbal medicines. Herein, the method was used to estimate the pharmacodynamics of Mai-Luo-Ning injection, a traditional Chinese compound herbal prescription. Through investigating the interactions between several important proteins in cardiovascular system and characteristic components of the formula, its effect on cardiovascular protection was evaluated. Results showed the differences in the interactions between each component and the selected target proteins and revealed the possible mechanisms for synergistic effects of various characteristic components on cardiovascular protection. The study provided scientific evidence supporting the mechanistic study of the interactions among multi-components and targets, offering a general approach to investigating the pharmacodynamics of complicated materials in compound herbal prescriptions.

  11. Serotype- and virulence-associated gene profile of Streptococcus suis isolates from pig carcasses in Chiang Mai Province, Northern Thailand

    PubMed Central

    WONGSAWAN, Kanruethai; GOTTSCHALK, Marcelo; THARAVICHITKUL, Prasit

    2014-01-01

    In this present study, the serotype of 40 Streptococcus suis isolates from submaxillary glands of pig carcasses sold in wet markets in Chiang Mai Province, northern Thailand, was investigated. Eleven serotypes, including types 2, 3, 4, 5, 7, 8, 9, 17, 21, 22 and 31, were found in the isolates by a Multiplex PCR combined with serum agglutination. Of the eleven serotypes present, type 3 was the most prevalent, while types 2, 4, 5 and 21 were of primary interest due to their human isolate serotype. The mrp+/epf − /sly − genotype was found to be the most prevalent genotype. This study indicates the importance of effective control of human S. suis infection due to raw pork or pig carcass handling in northern Thailand. PMID:25367105

  12. Visceral pentastomiasis caused by Armillifer armillatus in a captive striped hyena (Hyaena hyaena) in Chiang Mai Night Safari, Thailand.

    PubMed

    Dechkajorn, Sakorn; Nomsiri, Raksiri; Boonsri, Kittikorn; Sripakdee, Duanghatai; Sukontason, Kabkaew L; Wannasan, Anchalee; Chailangkarn, Sasisophin; Tiwananthagorn, Saruda

    2016-02-01

    Visceral pentastomiasis (porocephalosis) caused by Armillifer armillatus larvae was incidentally diagnosed in a female striped hyena (Hyaena hyaena) of unknown age which died unexpectedly in 2013. The hyena had been imported from Tanzania 8years earlier and have been since then in a zoo in Chiang Mai, northern Thailand. Pathological examination revealed visceral nymph migrans of pentastomes throughout the intestine, liver, diaphragm, omentum and mesentery, spleen, kidneys, and urinary bladder. Polymerase chain reaction and sequencing that targeted the pentastomid-specific 18S rRNA gene determined 100% identity with reference sequence for A. armillatus, suggesting that its ova can infect the hyena to serve as an intermediate host for the parasite. Further studies to identify the source of infection, its risk factors, and host range for A. armillatus are important to determine its zoonotic potential and to better prevent and manage the disease to protect animal and human health.

  13. Serum ASAT, ALAT, ALP, LD, GT, and CK determined in the Cobas-Bio centrifugal analyser by the methods of the Scandinavian Committee on Enzymes.

    PubMed

    Izquierdo, J M; Sotorrío, P; Alvarez-Uría, J; Estrada, J M; Quirós, A

    1982-04-01

    The recommended methods of the Scandinavian Committee on Enzymes [4, 5, 6, 7, 8] have been applied to the Cobas-Bio centrifugal analyser. Reagents and serum volumes were scaled down and final molarities were kept equal. Serum volumes in microliters were as follows: ASAT 30, ALAT 30, ALP 3, LD 5, GT 20, and CK 10. Including the dead space of the sample cup, the volume needed to perform all six tests was 113 microliter. Within-run and between-run precision (CV%) were as follows: ASAT 1.32 and 1.95, ALAT 1.68 and 2.93, ALP 1.56 and 3.10, LD 1.63 and 4.44, GT 0.81 and 2.23, and CK 1.02 and 1.94. Mean deviations (%) from target values of two commercial sera were as follows: ASAT -0.3 and -0.4, ALAT -0.4 and -2.2, ALP -1.8 and -7.3, LD 0.4 and -6.2, GT 13.9 and -10.7, and CK -4.9 and -1.3. Results of all the methods correlated well with those obtained with their respective manual methods. Analytical time for 28 samples of each analyte was 10 min, apart from CK which was 14 min. Reagent cost per sample was 0.6, 0.9, 0.1, 0.3, 0.9, and 26 US cents respectively. All reagents were prepared in the laboratory, except those for CK which were bought from J.T. Baker (Phillipsburgh, NJ, USA). In conclusion, the methods keep the features of the manual methods but they are more precise and practicable, much faster and cheaper, and use minimal amounts of sera more convenient for paediatric work.

  14. 58W LD side-pump Nd:YAG picosecond laser system at 1KHz with double length of regenerative cavity

    NASA Astrophysics Data System (ADS)

    Long, Ming Liang; Li, G.; Chen, Meng

    2016-11-01

    An average power of 58 W, pulse width of 40 ps at 1 KHz repetition rate of Nd:YAG picosecond laser is reported. It used an etalon to directly get pulse width of 135 ps from Nd:YVO4 mode locked laser in 1064 nm, which repetition rate was 88 MHz. When the seed pulses were injected into the double length of regenerative LD side-pump Nd:YAG cavity at 1 KHz repetition rate, the single pulse energy was amplified to 3 mJ, the pulse width was compressed to 99 ps, beam quality of M^2 factor was 1.3. The single pulse energy was amplified up to 58 mJ, the pulse width was self compressed to 40 ps, beam quality of M^2 factor was approximately 3.5 after single passing three stages of double high gain LD side-pump Nd:YAG module. Beam pointing was about 40 urad. The stability for pulse to pulse RMS was less than 3%. A thin-film polarizer and a quarter-wave plate was inserted into the regenerative amplification cavity to let pulses double travel the same geometric path basis on pulse polarization. Serrated aperture were used in the amplification. That's beneficial to decrease the nonlinear effect for the high power in the crystal. High gain LD side-pump Nd:YAG module could lead the pulse energy amplify more and self compress the pulse width. Double length of regenerative cavity was used to enhance the optical cavity length, it greatly decreased the laser's volume and improved stability of picosecond laser. It's a nice way for high power picosecond laser and the laser system would be more simple and smaller.

  15. Acute Oral Toxicity of Tetrodotoxin in Mice: Determination of Lethal Dose 50 (LD50) and No Observed Adverse Effect Level (NOAEL)

    PubMed Central

    Abal, Paula; Louzao, M. Carmen; Antelo, Alvaro; Alvarez, Mercedes; Cagide, Eva; Vilariño, Natalia; Vieytes, Mercedes R.; Botana, Luis M.

    2017-01-01

    Tetrodotoxin (TTX) is starting to appear in molluscs from the European waters and is a hazard to seafood consumers. This toxin blocks sodium channels resulting in neuromuscular paralysis and even death. As a part of the risk assessment process leading to a safe seafood level for TTX, oral toxicity data are required. In this study, a 4-level Up and Down Procedure was designed in order to determine for the first time the oral lethal dose 50 (LD50) and the No Observed Adverse Effect Level (NOAEL) in mice by using an accurate well-characterized TTX standard. PMID:28245573

  16. A Holocene Lake Record from Laguna Del Maule (LdM) in the Chilean Andes: Climatic and Volcanic Controls on Lake Depositional Dynamics

    NASA Astrophysics Data System (ADS)

    Valero-Garces, B. L.; Frugone Alvarez, M.; Barreiro-Lostres, F.; Carrevedo, M. L.; Latorre Hidalgo, C.; Giralt, S.; Maldonado, A.; Bernárdez, P.; Prego, R.; Moreno-Caballud, A.

    2014-12-01

    Central Chile is a tectonically active, drought-prone region sensitive to latitudinal variations in large-scale cold fronts associated with fluctuations of the Pacific subtropical high. Holocene high-resolution records of climate and volcanic events could help inform more on the frequency of extensive droughts as well as volcanic and seismic hazards. LdM is a high altitude, volcanic lake located in the Transition Southern Volcanic Zone (~36°S, 2200 m.a.s.l). The LdM volcanic field is a very seismically and volcanically active zone in the Andes, with several caldera-forming eruptions over the last 1.5 Ma, and intense postglacial activity. In 2013, we recovered over 40 m of sediment cores at four sites of LdM and collected > 20 km of seismic lines. The cores were imaged, their physical and geochemical properties analysed with a Geotek MSCL and XRF scanner respectively, and sampled for TOC, TIC, TS, TN, BioSi, and bulk mineralogy. The chronology was constructed with a Bayesian age-depth model including 210Pb-137Cs, the Quizapú volcanic ash (1932 AD) and 17 AMS 14C dates. The 4.8 m long composite sequence spans the Late glacial and Holocene.Sediments are massive to banded, quartz and plagioclase-rich silts with variable diatom (BioSi, 15- 30 %) and organic matter content (TOC, 1-5 %). Four main units have been defined based on sedimentological and geochemical composition. The transition from Unit 4 to 3 is ascribed to the onset of the Holocene; Unit 2 spans the mid Holocene, and Unit 1 the last 4 ka. Higher (lower) TOC, Br/Ti and Fe/Mn ratios in units 1 and 3 (2 and 4) suggest higher (lower) organic productivity in the lake and dominant oxic (anoxic) conditions at the bottom of the lake. Up to 17 ash and lapilli layers mark volcanic events, mostly grouped in units 1 and 3. Periods of higher lake productivity (units 1 and 3) are synchronous to higher frequency of volcanic events. Some climate transitions (LIA, 4ka, 8ka and 11ka) are evident in the LdM sequence

  17. Acute Oral Toxicity of Tetrodotoxin in Mice: Determination of Lethal Dose 50 (LD50) and No Observed Adverse Effect Level (NOAEL).

    PubMed

    Abal, Paula; Louzao, M Carmen; Antelo, Alvaro; Alvarez, Mercedes; Cagide, Eva; Vilariño, Natalia; Vieytes, Mercedes R; Botana, Luis M

    2017-02-24

    Tetrodotoxin (TTX) is starting to appear in molluscs from the European waters and is a hazard to seafood consumers. This toxin blocks sodium channels resulting in neuromuscular paralysis and even death. As a part of the risk assessment process leading to a safe seafood level for TTX, oral toxicity data are required. In this study, a 4-level Up and Down Procedure was designed in order to determine for the first time the oral lethal dose 50 (LD50) and the No Observed Adverse Effect Level (NOAEL) in mice by using an accurate well-characterized TTX standard.

  18. Coordinate expression of AOS genes and JA accumulation: JA is not required for initiation of closing layer in wound healing tubers

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Wounding induces a series of coordinated physiological responses essential for protection and healing of the damaged tissue. Wound-induced formation of jasmonic acid (JA) is important in defense responses in leaves, but comparatively little is known about the induction of JA biosynthesis and its ro...

  19. Exergy analysis of a counter flow Ranque-Hilsch vortex tube for different cold orifice diameters, L/D ratios and exit valve angles

    NASA Astrophysics Data System (ADS)

    Devade, Kiran D.; Pise, Ashok T.

    2016-12-01

    An experimental investigation is made to find out the effects of the cold end orifice diameters, length to diameter ratio and exit valve angles on the heating and cooling performance of the counter flow Ranque-Hilsch vortex tube with air as a working fluid. The tube and cold end orifices used at these experiments are made of brass. Three cold end orifices (5, 6 and 7 mm) have been manufactured and are used five different L/D ratios (15 plain tube, 15-18 with 4° divergence angle) and exit valve angles (30°-90°). Inlet pressures were adjusted from 200 to 600 kPa with 100 kPa increments, and the exergy loss, exergy efficiency was determined. As a result of the experimental study, it is determined that the exergy loss between the hot and cold fluid is decreased with increasing of the cold end orifice diameter. Exergy efficiency decreases with increase in L/D ratio. It is also concluded that diverging vortex tube produces lower exergy loss as compared to plain tube. Valve angles have significant effect on hot end exergy loss of the vortex tube.

  20. The west side story: MEFV haplotype in Spanish FMF patients and controls, and evidence of high LD and a recombination "hot-spot" at the MEFV locus.

    PubMed

    Aldea, Anna; Calafell, Francesc; Aróstegui, Juan I; Lao, Oscar; Rius, Josefa; Plaza, Susana; Masó, Montserrat; Vives, Jordi; Buades, Joan; Yagüe, Jordi

    2004-04-01

    Mutations at the MEFV gene cause, with various degrees of penetrance, familial Mediterranean fever (FMF). This disease is more prevalent in the Middle East than elsewhere, and most studies have focused on those populations. However, FMF occurs also in the Western Mediterranean and these populations should be taken into account for a complete view of FMF. We have analyzed intragenic MEFV SNPs in Spanish and Chueta (descendants of converted Jews) FMF patients and controls, and this constitutes the first systematic survey of normal MEFV SNP haplotype structure and variability. Our findings have allowed us to systematize the nomenclature of MEFV haplotypes and show that there is strong linkage disequilibrium (LD) at the MEFV locus and an intragenic recombination hot spot. The high local LD, regardless the recombination hot spot, is responsible for the limited diversity of the MEFV control haplotypes found in the Spanish population and it suggests that it may be a common feature to all Mediterranean populations. The MEFV mutation spectrum in Spain is quite diverse, and similar to those of France and Italy. On the contrary, the Chueta spectrum was poorer and closer to that of North African Jews, suggesting a direct connection with the Jewish diaspora.

  1. 1.5-μm and 10-Gb s-1 etched mesa buried heterostructure DFB-LD for datacenter networks

    NASA Astrophysics Data System (ADS)

    Kwon, Oh Kee; Lee, Chul Wook; Leem, Young Ahn; Kim, Ki Soo; Oh, Su Hwan; Nam, Eun Soo

    2015-10-01

    We report a 1.5 μm and 10 Gb s-1 etched mesa buried heterostructure λ/4-shifted distributed feedback laser diode (DFB-LD) for the low-cost application of WDM-based datacenter networks. To reduce the threshold current and improve the modulation bandwidth in a conventional p-/n-/p-InP current blocking structure, a thin undoped-InP (u-InP) layer was inserted between the side walls of the active region and the p-InP layer (i.e., a u-/p-/n-/p-InP structure), and the region containing the active region and the current blocking structures was etched in a mesa form (i.e., an etched mesa). From this work, it was found that a 300 μm long anti-reflection (AR)-AR DFB-LD with a mesa width of 8 μm is reduced by about 25% while a side mode suppression ratio is >50 dB and a 3 dB bandwidth is >10 GHz at a current of 40 mA; in addition, it shows a clear eye-opening with a dynamic extinction ratio of >4.5 dB at 10 Gb s-1, and a power penalty of <1 dB after a 2 km transmission.

  2. Gastrointestinal syndrome and mucosal clonogenic cells: relationships between target cell sensitivities, LD/sub 50/ and cell survival, and their modification by antibiotics

    SciTech Connect

    Hendry, J.H.; Potten, C.S.; Roberts, N.P.

    1983-10-01

    The sensitivity of the target cells responsible for the gastrointestinal syndrome in mice was deduced from the steepness of the dose-survival curve for mice assessed on Day 7 after irradiation. The D/sub 0/ value was 1.25 +- 0.22 Gy, virtually identical to the value of 1.23 +- 0.08 measured for microcolony-forming cells (clonogens) over about the same range of dose in concurrent experiments. The survival of clonogens was similar when assayed in mice surviving to Days 3, 4, or 5, but clonogenic sensitivity was lower when assessed on Day 7. This was shown at one dose to be due largely to a selection of mice with high colony counts with only a small contribution from crypt budding. The LD/sub 50/ for mice corresponded to a surviving fraction of crypts of about 0.35. An injection of 5 mg streptomycin sulphate ip daily for 5 days after irradition increased the latent period by about 1 day, increased the LD/sub 50/ by about 1.4 Gy, but did not significantly change the survival of clonogens. These studies are the first to test and satisfy the interpretation of a dose-response curve for animal survival in terms of target cell survival, where measurements of both are made over a similar range of dose in concurrent experiments.

  3. Reduction of mode partition noise of FP-LD by using Mach-Zehnder interferometer for RSOA-based DWDM applications.

    PubMed

    Yoo, Sang-Hwa; Moon, Sang-Rok; Kye, Myeonggyun; Lee, Chang-Hee

    2016-06-27

    We investigate reduction of mode partition noise of a spectrally sliced Fabry-Perot laser diode (FP-LD) for application to seeded DWDM systems. The proposed scheme for the noise reduction incorporates a fiber-based Mach-Zehnder interferometer (MZI) and a reflective semiconductor optical amplifier (RSOA). The MZI enables to reduce a relative intensity noise (RIN) more than 3 dB with better noise distributions. Experimental results of 10-Gb/s signal transmission exhibit a considerable bit-error-rate (BER) reduction by three orders of magnitude at the given received power. After the noise reduction, the FP-LD is applied to a 10-Gb/s DWDM system as a seed-light-source. In a local-seeding scheme, return-to-zero (RZ) and carrier-suppressed (CS)-RZ signal formats are compared as a function of transmission distance. Furthermore, a back-reflection induced impairment is evaluated in a remote-seeding scheme. We also count the number of useable channels to show the feasibility of DWDM transmission.

  4. Effects and safety profile of betahistine in patients in the Russian contingent of OSVaLD, an open-label observational study in vestibular vertigo

    PubMed Central

    Morozova, Svetlana Vyacheslavovna; Alekseeva, Natalia Stepanovna; Lilenko, Sergey Vasilyevich; Matsnev, Eduard Ivanovich; Melnikov, Oleg Anatol’evich

    2015-01-01

    Background We report here data from the >200 patients recruited in Russia to take part in OSVaLD, a 12-week, open-label, post-marketing surveillance study of the response to betahistine 48 mg/day in vertigo of peripheral vestibular origin carried out in a total of 13 countries. Methods The primary efficacy endpoint was change in the Dizziness Handicap Inventory (DHI; 100-point scale). Changes in Hospital Anxiety and Depression Scale (HADS) and Medical Outcomes Study Short-Form 36, version 2 (SF-36v2®) scores were a priori secondary Outcomes. Results Total DHI score improved by 43 points during betahistine treatment. This aggregate improvement was equally distributed across the three domains of the DHI (physical, emotional, and functional; P<0.0001 for main and subscore changes from baseline). Statistically significant improvements versus baseline were also observed in mean HADS scores for anxiety and depression (both P<0.0001), and in the Physical Component Summary and Mental Component Summary scores of the SF-36v2 (both P<0.0001 versus baseline). Only one suspected adverse drug reaction was recorded in the Russian safety population (n=204), indicating that betahistine was well tolerated in those patients. Conclusion Betahistine 48 mg/day was associated with clear improvements in well-configured and widely validated measures of health-related quality of life and an encouraging tolerability profile in patients in Russia who took part in OSVaLD. PMID:25653552

  5. Protective Effects of Sheng-Mai-San on Right Ventricular Dysfunction during Chronic Intermittent Hypoxia in Mice

    PubMed Central

    Chai, Cheng-Zhi; Mo, Wei-Lan; Zhuang, Xian-Fei; Yan, Yong-Qing

    2016-01-01

    Right ventricular (RV) dysfunction and failure contribute to the increasing morbidity and mortality of cardiovascular diseases; however, current treatment strategies are grossly inadequate. Sheng-Mai-San (SMS) has been used to treat heart diseases for hundreds of years in China, and its protective effects on RV have not been observed. The present study was to investigate the protective effects of SMS aqueous extract on RV dysfunction in chronic intermittent hypoxia (CIH) mice model. The results showed that CIH mice model presented RV dysfunction and maladaptive compensation after 28-day-CIH and SMS treatment significantly reversed these changes. Diastolic function of RV was restored and systolic dysfunction was attenuated, including elevation of RV stroke volume and fractional shortening, as well as pulmonary circulation. Structurally, SMS treatment inhibited RV dilation, cardiomyocytes vacuolization, ultrastructure abnormalities, and the expression of cleaved caspase-3. Of importance, SMS showed remarkable antioxidant activity by decreasing the levels of malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE), increasing the levels of superoxide dismutase (SOD) and heme oxygenase-1 (HO-1), as well as inhibiting the overexpression of 3-NT in RV. Our results indicate that SMS preserve RV structure and function in CIH-exposed mice by involving regulation in both ROS and Reactive Nitrogen Species (RNS) production. PMID:27073402

  6. Traditional Chinese medicine xin-mai-jia recouples endothelial nitric oxide synthase to prevent atherosclerosis in vivo.

    PubMed

    Yin, Ya-Ling; Zhu, Mo-Li; Wan, Jia; Zhang, Chong; Pan, Guo-Pin; Lu, Jun-Xiu; Ping, Song; Chen, Yuan; Zhao, Fan-Rong; Yu, Hai-Ya; Guo, Tao; Jian, Xu; Liu, Li-Ying; Zhang, Jia-Ning; Wan, Guang-Rui; Wang, Shuang-Xi; Li, Peng

    2017-03-02

    Endothelial dysfunction, which is caused by endothelial nitric oxide synthase (eNOS) uncoupling, is an initial step in atherosclerosis. This study was designed to explore whether Chinese medicine xin-mai-jia (XMJ) recouples eNOS to exert anti-atherosclerotic effects. Pretreatment of XMJ (25, 50, 100 μg/ml) for 30 minutes concentration-dependently activated eNOS, improved cell viabilities, increased NO generations, and reduced ROS productions in human umbilical vein endothelial cells incubated with H2O2 for 2 hours, accompanied with restoration of BH4. Importantly, these protective effects produced by XMJ were abolished by eNOS inhibitor L-NAME or specific eNOS siRNA in H2O2-treated cells. In ex vivo experiments, exposure of isolated aortic rings from rats to H2O2 for 6 hours dramatically impaired acetylcholine-induced vasorelaxation, reduced NO levels and increased ROS productions, which were ablated by XMJ in concentration-dependent manner. In vivo analysis indicated that administration of XMJ (0.6, 2.0, 6.0 g/kg/d) for 12 weeks remarkably recoupled eNOS and reduced the size of carotid atherosclerotic plaque in rats feeding with high fat diet plus balloon injury. In conclusion, XMJ recouples eNOS to prevent the growth of atherosclerosis in rats. Clinically, XMJ is potentially considered as a medicine to treat patients with atherosclerosis.

  7. Traditional Chinese medicine xin-mai-jia recouples endothelial nitric oxide synthase to prevent atherosclerosis in vivo

    PubMed Central

    Yin, Ya-Ling; Zhu, Mo-Li; Wan, Jia; Zhang, Chong; Pan, Guo-Pin; Lu, Jun-Xiu; Ping, Song; Chen, Yuan; Zhao, Fan-Rong; Yu, Hai-Ya; Guo, Tao; Jian, Xu; Liu, Li-Ying; Zhang, Jia-Ning; Wan, Guang-Rui; Wang, Shuang-Xi; Li, Peng

    2017-01-01

    Endothelial dysfunction, which is caused by endothelial nitric oxide synthase (eNOS) uncoupling, is an initial step in atherosclerosis. This study was designed to explore whether Chinese medicine xin-mai-jia (XMJ) recouples eNOS to exert anti-atherosclerotic effects. Pretreatment of XMJ (25, 50, 100 μg/ml) for 30 minutes concentration-dependently activated eNOS, improved cell viabilities, increased NO generations, and reduced ROS productions in human umbilical vein endothelial cells incubated with H2O2 for 2 hours, accompanied with restoration of BH4. Importantly, these protective effects produced by XMJ were abolished by eNOS inhibitor L-NAME or specific eNOS siRNA in H2O2-treated cells. In ex vivo experiments, exposure of isolated aortic rings from rats to H2O2 for 6 hours dramatically impaired acetylcholine-induced vasorelaxation, reduced NO levels and increased ROS productions, which were ablated by XMJ in concentration-dependent manner. In vivo analysis indicated that administration of XMJ (0.6, 2.0, 6.0 g/kg/d) for 12 weeks remarkably recoupled eNOS and reduced the size of carotid atherosclerotic plaque in rats feeding with high fat diet plus balloon injury. In conclusion, XMJ recouples eNOS to prevent the growth of atherosclerosis in rats. Clinically, XMJ is potentially considered as a medicine to treat patients with atherosclerosis. PMID:28252100

  8. POTENTIALLY PATHOGENIC FREE-LIVING AMOEBAE IN SOME FLOOD-AFFECTED AREAS DURING 2011 CHIANG MAI FLOOD

    PubMed Central

    Wannasan, Anchalee; Uparanukraw, Pichart; Songsangchun, Apichart; Morakote, Nimit

    2013-01-01

    SUMMARY The survey was carried out to investigate the presence of potentially pathogenic free-living amoebae (FLA) during flood in Chiang Mai, Thailand in 2011. From different crisis flood areas, seven water samples were collected and tested for the presence of amoebae using culture and molecular methods. By monoxenic culture, FLA were detected from all samples at 37 °C incubation. The FLA growing at 37 °C were morphologically identified as Acanthamoeba spp., Naegleria spp. and some unidentified amoebae. Only three samples (42.8%), defined as thermotolerant FLA, continued to grow at 42 °C. By molecular methods, two non-thermotolerant FlA were shown to have 99% identity to Acanthamoeba sp. and 98% identity to Hartmannella vermiformis while the two thermotolerant FLA were identified as Echinamoeba exundans (100% identity) and Hartmannella sp. (99% identity). This first report of the occurrence of FLA in water during the flood disaster will provide information to the public to be aware of potentially pathogenic FLA. PMID:24213194

  9. Potentially pathogenic free-living amoebae in some flood-affected areas during 2011 Chiang Mai flood.

    PubMed

    Wannasan, Anchalee; Uparanukraw, Pichart; Songsangchun, Apichart; Morakote, Nimit

    2013-01-01

    The survey was carried out to investigate the presence of potentially pathogenic free-living amoebae (FLA) during flood in Chiang Mai, Thailand in 2011. From different crisis flood areas, seven water samples were collected and tested for the presence of amoebae using culture and molecular methods. By monoxenic culture, FLA were detected from all samples at 37 °C incubation. The FLA growing at 37 °C were morphologically identified as Acanthamoeba spp., Naegleria spp. and some unidentified amoebae. Only three samples (42.8%), defined as thermotolerant FLA, continued to grow at 42 °C. By molecular methods, two non-thermotolerant FlA were shown to have 99% identity to Acanthamoeba sp. and 98% identity to Hartmannella vermiformis while the two thermotolerant FLA were identified as Echinamoeba exundans (100% identity) and Hartmannella sp. (99% identity). This first report of the occurrence of FLA in water during the flood disaster will provide information to the public to be aware of potentially pathogenic FLA.

  10. Investigation of the Effects and Mechanisms of Mai Tong Formula on Lower Limb Macroangiopathy in a Spontaneous Diabetic Rat Model

    PubMed Central

    Yuan, Haipo; Liu, Ya; Qi, Luguang

    2016-01-01

    A new Chinese herbal formula called Mai Tong Formulae (MTF) has recently been used to treat lower limb macroangiopathy in type 2 diabetes mellitus (T2DM) patients. In this study, we investigated the effect of MTF on lower limb macroangiopathy in a spontaneous diabetic rat model (GK rats). We found that MTF treatment significantly reduced serum fasting blood glucose (FBG), triglycerides (TG), total cholesterol (TC), IL6, and VEGF and increased serum insulin in this model. Histological and ultrastructural observations showed that MTF treatment significantly reduced vascular endothelial cell shedding and improved endothelium injuries. We further detect proteome alteration following MTF treatment. 25 differential proteins (DPs) abnormally expressed in GK rats were normalized by MTF treatment. These DPs significantly are enriched in biological processes and pathways that regulate muscle contraction and cGMP-PKG signaling pathway and so on. Additional protein-protein interaction (PPI) network analyses of the DPs showed that Fasn and Prkar2a are involved in the AMPK signaling pathway, and Gnas, Myh11, and Myh6 are involved in vascular smooth muscle contraction; these 5 DPs were validated by Western blotting. These results indicate that MTF treatment effectively treats lower limb macroangiopathy by regulating key proteins involved in AMPK signaling pathway and vascular smooth muscle contraction. PMID:27995148

  11. Petrological Features of The Post-Collisional Volcanics on the Izmir-Ankara-Erzincan Suture Zone at Around Almus and Yıldızeli Regions, NE Turkey.

    NASA Astrophysics Data System (ADS)

    Göçmengil, G.; Karacik, Z.; Genç, Ş. C.

    2014-12-01

    Following the closure of the northern Neo-Tethyan ocean, post-collisional magmatism developed both on the Izmir-Ankara-Erzincan suture zone (IAESZ) and the northern (Pontides) and southern (Kırşehir block) continents. Cenozoic volcanics (particularly Middle to Upper Eocene) from the Almus and Yıldızeli regions (NE, Turkey) have poorly known and exposed along the northern and southern part of the IAESZ respectively. In this presentation; we present geological, geochemical and isotopic data of these volcanic rocks to characterize the petrological evolution and petrogenesis on the different tectonic blocks. Almus region, have a composite basement of Mesozoic metamorphic and ophiolitic rocks. Volcanic and sedimentary units of the Middle Eocene covering the basement units have a stratigraphic sequence of: a basal conglomerate; fossiliferous sandstone/shale; epiclastic rocks; auto-brecciated lavas with basalt/basaltic andesite lava flows. All these units are cut by trachyte stocks. Besides, basement of Yıldızeli region consisting of Cretaceous Kırşehir block metamorphics with IAESZ melangé and flysch units. Volcanic and sedimentary units covers the basement are trachyte-trachyandesite lavas; fossiliferous limestone; basalt/basaltic andesite lava flows; pyroclastic units with dacitic lavas. Basalt/basaltic andesites from Almus region are sub-alkaline/mildly alkaline and display middle to high-K character. REE data display relative enrichments in LILE (Sr, K, Rb, Ba, Th) together with negative anomalies of Nb, Ta and P. Trachyte stocks are shoshonitic and display LILE enrichment together with negative patters of Nb, Ta, P and Eu. Besides, trachyte-trachyandesite and basalt/basaltic andesite from Yıldızeli region are sub-alkaline/mildly alkaline with middle-K to shoshonitic character. REE patterns show enrichments in LILE and depletion of Nb, Ta, P and Ti for both rock suites. The 87Sr/86Sr and 143Nd/144Nd ratios of volcanic rocks from both regions, range from 0

  12. The mealybug Phenacoccus solenopsis suppresses plant defense responses by manipulating JA-SA crosstalk

    PubMed Central

    Zhang, Peng-Jun; Huang, Fang; Zhang, Jin-Ming; Wei, Jia-Ning; Lu, Yao-Bin

    2015-01-01

    Induced plant defenses against herbivores are modulated by jasmonic acid-, salicylic acid-, and ethylene-signaling pathways. Although there is evidence that some pathogens suppress plant defenses by interfering with the crosstalk between different signaling pathways, such evidence is scarce for herbivores. Here, we demonstrate that the mealybug Phenacoccus solenopsis suppresses the induced defenses in tomato. We found that exogenous JA, but not SA, significantly decreased mealybug feeding time and reduced nymphal performance. In addition, constitutive activation of JA signaling in 35s::prosys plants reduced mealybug survival. These data indicate that the JA signaling pathway plays a key role in mediating the defense responses against P. solenopsis. We also found that mealybug feeding decreased JA production and JA-dependent defense gene expression, but increased SA accumulation and SA-dependent gene expression. In SA-deficient plants, mealybug feeding did not suppress but activated JA accumulation, indicating that the suppression of JA-regulated defenses depends on the SA signaling pathway. Mealybugs benefit from suppression of JA-regulated defenses by exhibiting enhanced nymphal performance. These findings confirm that P. solenopsis manipulates plants for its own benefits by modulating the JA-SA crosstalk and thereby suppressing induced defenses. PMID:25790868

  13. Synthesis, structural characterization and biological activity of two diastereomeric JA-Ile macrolactones.

    PubMed

    Jimenez-Aleman, Guillermo H; Machado, Ricardo A R; Görls, Helmar; Baldwin, Ian T; Boland, Wilhelm

    2015-06-07

    Jasmonates are phytohormones involved in a wide range of plant processes, including growth, development, senescence, and defense. Jasmonoyl-L-isoleucine (JA-Ile, 2), an amino acid conjugate of jasmonic acid (JA, 1), has been identified as a bioactive endogenous jasmonate. However, JA-Ile (2) analogues trigger different responses in the plant. ω-Hydroxylation of the pentenyl side chain leads to the inactive 12-OH-JA-Ile (3) acting as a “stop” signal. On the other hand, a lactone derivative of 12-OH-JA (5) (jasmine ketolactone, JKL) occurs in nature, although with no known biological function. Inspired by the chemical structure of JKL (6) and in order to further explore the potential biological activities of 12-modified JA-Ile derivatives, we synthesized two macrolactones (JA-Ile-lactones (4a) and (4b)) derived from 12-OH-JA-Ile (3). The biological activity of (4a) and (4b) was tested for their ability to elicit nicotine production, a well-known jasmonate dependent secondary metabolite. Both macrolactones showed strong biological activity, inducing nicotine accumulation to a similar extent as methyl jasmonate does in Nicotiana attenuata leaves. Surprisingly, the highest nicotine contents were found in plants treated with the JA-Ile-lactone (4b), which has (3S,7S) configuration at the cyclopentanone not known from natural jasmonates. Macrolactone (4a) is a valuable standard to explore for its occurrence in nature.

  14. Animal mortality resulting from uniform exposures to photon radiations: Calculated LD/sub 50/s and a compilation of experimental data

    SciTech Connect

    Jones, T.D.; Morris, M.D.; Wells, S.M.; Young, R.W.

    1986-12-01

    Studies conducted during the 1950s and 1960s of radiation-induced mortality to diverse animal species under various exposure protocols were compiled into a mortality data base. Some 24 variables were extracted and recomputed from each of the published studies, which were collected from a variety of available sources, primarily journal articles. Two features of this compilation effort are (1) an attempt to give an estimate of the uniform dose received by the bone marrow in each treatment so that interspecies differences due to body size were minimized and (2) a recomputation of the LD/sub 50/ where sufficient experimental data are available. Exposure rates varied in magnitude from about 10/sup -2/ to 10/sup 3/ R/min. This report describes the data base, the sources of data, and the data-handling techniques; presents a bibliography of studies compiled; and tabulates data from each study. 103 refs., 44 tabs.

  15. Experimental basis of standardized specimen collection: the effect of short moderate exercise on serum K, Na, ASAT, ALAT, CK and LD.

    PubMed

    Leppänen, E A

    1989-05-01

    Strenuous exercise is known to produce marked changes in many serum components. Present recommendations concerning drawing of blood for laboratory tests, therefore, allow little exercise before specimen collection. However, the effects of moderate exercise are unknown. Moderate exercise administered by bicycle ergometer (120-160 W for 15 min) to healthy persons and ambulant patients (average 107 W) induced a rapid and statistically significant effect on serum potassium, sodium, ASAT, CK and LD concentrations. However, the observed changes were largely abolished by having the patient to sit for 15 min before venipuncture. It appears to be possible to allow moderate exercise before venipuncture as long as the specimen collection procedure itself is sufficiently standardized, i.e. by specifying sitting for 15 min and no use of tourniquet.

  16. Aerosol transport from Chiang Mai, Thailand to Mt. Lulin, Taiwan - Implication of aerosol aging during long-range transport

    NASA Astrophysics Data System (ADS)

    Chuang, Ming-Tung; Lee, Chung-Te; Chou, Charles C.-K.; Engling, Guenter; Chang, Shih-Yu; Chang, Shuenn-Chin; Sheu, Guey-Rong; Lin, Neng-Huei; Sopajaree, Khajornsak; Chang, You-Jia; Hong, Guo-Jun

    2016-07-01

    The transport of biomass burning (BB) aerosol from Indochina may cause a potential effect on climate change in Southeast Asia, East Asia, and the Western Pacific. Up to now, the understanding of BB aerosol composition modification during long-range transport (LRT) is still very limited due to the lack of observational data. In this study, atmospheric aerosols were collected at the Suthep/Doi Ang Khang (DAK) mountain sites in Chiang Mai, Thailand and the Lulin Atmospheric Background Station (Mt. Lulin) in central Taiwan from March to April 2010 and from February to April 2013, respectively. During the study period, an upwind and downwind relationship between the Suthep/DAK and Lulin sites (2400 km apart) was validated by backward trajectories. Comprehensive aerosol properties were resolved for PM2.5 water-soluble inorganic ions, carbonaceous content, water-soluble/insoluble organic carbon (WSOC/WIOC), dicarboxylic acids and their salts (DCAS), and anhydrosugars. A Modification Factor (MF) is proposed by employing non-sea-salt potassium ion (nss-K+) or fractionalized elemental carbon evolved at 580 °C after pyrolized OC correction (EC1-OP) as a BB aerosol tracer to evaluate the mass fraction changes of aerosol components from source to receptor regions during LRT. The MF values of nss-SO42-, NH4+, NO3-, OC1 (fractionalized organic carbon evolved from room temperature to 140 °C), OP (pyrolized OC fraction), DCAS, and WSOC were above unity, which indicated that these aerosol components were enhanced during LRT as compared with those in the near-source region. In contrast, the MF values of anhydrosugars ranged from 0.1 to 0.3, indicating anhydrosugars have degraded during LRT.

  17. Distribution patterns of polycyclic aromatic hydrocarbons (PAHs) in the sediments and fish at Mai Po Marshes Nature Reserve, Hong Kong.

    PubMed

    Liang, Y; Tse, M F; Young, L; Wong, M H

    2007-03-01

    Sediment samples were collected monthly from eight shrimp shallow ponds (local name gei wais) from July 2003 to January 2004, and from mangrove swamps and inter-tidal mudflats in July and November 2003, respectively. Fish samples (tilapia) were also collected. Polycyclic aromatic hydrocarbons (PAHs) were analyzed by gas chromatography and mass spectrometry (GC/MS). The results indicated that under wet season wet deposition and suspended particulates brought in by nearby rivers, such as the Peal River, served as an important source of PAHs entering Mai Po Marshes. Total organic matter in the sediments showed significant correlations (p<0.01) with PAHs in the sediments, mainly due to the mechanism that organic matter such as humic substances increased PAH persistence by binding and occluding PAHs. Except for naphthalene, biota-sediment accumulation factors (BSAF) of the PAHs in tilapia were below 1.7, which may be caused by biotransformation and the lower uptake in fish. In addition, aqueous route dominated accumulation of non-biodegradable PAHs in tilapia because higher levels were detected in larger fish than in smaller ones. A general trend was observed that BSAFs declined with the increase of K(ow) values, which suggested that bioavailability of low K(ow) isomers was high due to higher gill transfer efficiencies (aqueous uptake) in fish but enhanced biotransformation and decreased gut assimilation (dietary uptake) resulted in decreased accumulation of more hydrophobic PAHs (high K(ow)). Lastly, viscera appeared to be a promising tissue for biomonitoring, as it contained much higher concentrations than the muscle (3.5 magnitudes), and the levels in the muscle were significantly correlated with those in the viscera (r2=0.938, p<0.0001).

  18. Changes in urinary Cu, Zn, and Se levels in cancer patients after treatment with Sha Shen Mai Men Dong Tang

    PubMed Central

    Lai, Tung-Yuan; Kuo, Hsien-Wen

    2015-01-01

    Sha Shen Mai Men Dong Tang (SMD-2; 沙參麥冬湯 shā shēn mài dōng tāng) is a Chinese medicinal herb (CMH; 中草藥 zhōng cǎo yào) used to treat symptoms associated with cancer therapy. The objective of this study was to assess the effect of SMD-2 on the levels of urinary copper (Cu), zinc (Zn), and selenium (Se) in lung cancer patients and head and neck cancer patients receiving chemoradiotherapy. Forty-two head and neck cancer patients and 10 lung cancer patients participated in our clinical trial. Each patient received chemoradiotherapy for 4 weeks. In addition, each patient was treated with SMD-2 for 8 weeks, including 2 weeks prior to and after the chemoradiotherapy treatment. Comparison of urinary Cu, Zn, and Se levels and the ratios of Zn to Cu and Se to Cu at three time points in the two types of cancer were assessed using the generalized estimating equations (GEEs). After the patients received chemoradiotherapy for 4 weeks, SMD-2 treatment was found to be associated with a significant decrease in urinary Cu levels, whereas urinary Zn and Se levels increased significantly. In addition, the ratios of Zn to Cu and Se to Cu in the urine samples of these patients also increased significantly. Both the urinary Zn levels and the ratio of Zn to Cu in head and neck cancer patients were significantly higher than in lung cancer patients. Urinary Zn and Se levels and the ratios of Zn to Cu and Se to Cu, but not urinary Cu levels, increased significantly during and after treatment when assessed using the GEE model. The SMD-2 treatments significantly increased Zn and Se levels in the urine of head and neck cancer patients. Increased Zn and Se levels in urine strengthened immune system. PMID:27114935

  19. Incidence of short-term complications and associated factors after primary trabeculectomy in Chiang Mai University Hospital

    PubMed Central

    Leeungurasatien, Thidarat; Khunsongkiet, Preeyanuch; Pathanapitoon, Kassara; Wiwatwongwana, Damrong

    2016-01-01

    Background: To determine the incidence of early postoperative complications and associated factors after primary trabeculectomy in Thai glaucoma patients. Design: This was a retrospective observational study performed in Chiang Mai University Hospital. Participants: One hundred and eighteen glaucoma patients participated in the study. Methods: All glaucoma patients underwent primary trabeculectomy with mitomycin C (MMC) using fornix-based conjunctival flap technique between December 2011 and May 2013. Surgical complications during the first 3 months of follow-up were recorded, and associated risk factors were analyzed. Main Outcome Measures: The incidence of posttrabeculectomy complications was the main outcome measure. Results: One hundred and eighteen eyes of 118 patients were included. Early postoperative complications developed in 55 eyes (56.7%). Complications included hypotony (25 eyes, 27.2%), serous choroidal detachment (CD) (14 eyes, 15.6%), subconjunctival hemorrhage (12 eyes, 13.0%), hyphema (11 eyes, 12.4%), bleb leak (8 eyes, 8.8%), encapsulated bleb (2 eyes, 2.2%), aqueous misdirection (1 eyes, 1.1%), corneal epithelial defect (1 eyes, 1.1%), and overfiltration (1 eyes, 1.1%). There were no reported cases of endophthalmitis or blebitis. Hypotony was associated with serous CD (P = 0.006), and hyphema was associated with neovascular glaucoma (NVG) patients (P = 0.009). NVG was not associated with the increased rate of surgical failure (P = 0.083). Conclusions: The incidence of early complications after first-time trabeculectomy with MMC was high (56.7%) in this Thai clinic setting, but most were transient and self-limited conditions. The correlations between hypotony and CD as well as hyphema and NVG were compatible with the previous studies. PMID:27905335

  20. Alcohol intoxication in road traffic accidents leads to higher impact speed difference, higher ISS and MAIS, and higher preclinical mortality.

    PubMed

    Stübig, Timo; Petri, Maximilian; Zeckey, Christian; Brand, Stephan; Müller, Christian; Otte, Dietmar; Krettek, Christian; Haasper, Carl

    2012-11-01

    Alcohol is one of the most important personal risk factors for serious and fatal injuries, contributing to approximately one third of all deaths from accidents. It is also described that alcohol intoxication leads to a higher mortality in the clinical course. In this study, we hypothesized that alcohol intoxication leads to different accident kinematics, a higher ISS (Injury Severity Score), and higher preclinical mortality compared to sober patients. A technical and medical investigation of alcohol intoxicated road users was performed on the scene of the crash and at the primary admitting hospital. Alcohol testing was performed with either breath alcohol tests or measurement of blood alcohol concentration (BAC) in a standard laboratory test. Between 1999 and 2010, 37,635 road traffic accidents were evaluated by the Accident Research Unit. Overall 20,741 patients were injured, 2.3% of the patients were killed. Among the injured patients, 2.2% with negative BAC were killed, compared to 4.6% fatal injuries in patients with a positive BAC (p < 0.0001). Of the patients with a positive BAC, 8.0% were severely injured, compared to 3.6% in the BAC negative group (p < 0.0001). Regarding the relative speed at impact (Δv for motorized drivers, vehicle collision speed for pedestrians and bikers), there was a significant higher difference for BAC positive patients (30 ± 20) compared to the BAC negative patients (25 ± 19, p < 0.0001). Alcohol intoxication in trauma patients leads to higher preclinical mortality, higher impact speed difference, and higher injury severity. The subgroup analysis for different alcohol concentrations shows no difference in ISS, MAIS, and relative speed, but a correlation of increasing age of patients with higher alcohol concentrations.

  1. Prevalence of parasitism among students of the Karen hill-tribe in Mae Chame district, Chiang Mai province, Thailand.

    PubMed

    Saksirisampant, Wilai; Prownebon, Jarruratt; Kanmarnee, Penjit; Thaisom, Sunida; Yenthakam, Sutin; Nuchprayoon, Surang

    2004-09-01

    Infection caused by intestinal parasites is still a common health problem in a poor-hygiene population especially for children in developing countries. A cross-sectional study was conducted among 781 Karen students (age: 3 to 19, males: 325, females: 456) to determine the current status of intestinal parasitic infections in a mountainous area in the North of Thailand. The study was drawn from three schools in the Doi Inthanon region, in Mae Chame district of Chiang Mai province, from December 2002 to June 2003. The techniques used for the diagnosis were: stool concentration by using the 'formalin-ether' technique and perianal region examination by using the 'Scotch-tape' technique. The average rate of intestinal parasites for the group tested using the stool concentration technique was 42.06% (male: 46.87%, females: 38.82%); and 22.66% (males: 28.92%, females: 18.20%) when using the Scotch-tape technique.Among helminth-infected individuals, enterobiasis was found at the highest prevalence (15.49%). Other common infections were ascariasis (9.78%), trichuriasis (5.90%) and hookworm infection (2.20%). Strongyloildiasis was found only in 0.92%. For protozoa infection, the major cause is the non-pathogenic species "Entamoeba coli" (27.68%). The other non-pathogenic protozoa (Endolimax nana, Chilomastix meslini and Iodamoeba butschlii) had a low prevalence from ranged 0.18%-4.79%. The prevalence of pathogenic Giardia lamblia infection was 2.21%. Entamoeba histolytica infection was found in only one case. Based on the two techniques used, the results from the Scotch-tape provided a higher sensitivity for the detection of Taenia spp. and Enterobius vermicularis eggs. Drug treatment was given to all the infected students. School-based health education should be implemented in order to prevent and control the infections.

  2. Changes in urinary Cu, Zn, and Se levels in cancer patients after treatment with Sha Shen Mai Men Dong Tang.

    PubMed

    Lai, Tung-Yuan; Kuo, Hsien-Wen

    2016-04-01

    Sha Shen Mai Men Dong Tang (SMD-2; shā shēn mài dōng tāng) is a Chinese medicinal herb (CMH; zhōng cǎo yào) used to treat symptoms associated with cancer therapy. The objective of this study was to assess the effect of SMD-2 on the levels of urinary copper (Cu), zinc (Zn), and selenium (Se) in lung cancer patients and head and neck cancer patients receiving chemoradiotherapy. Forty-two head and neck cancer patients and 10 lung cancer patients participated in our clinical trial. Each patient received chemoradiotherapy for 4 weeks. In addition, each patient was treated with SMD-2 for 8 weeks, including 2 weeks prior to and after the chemoradiotherapy treatment. Comparison of urinary Cu, Zn, and Se levels and the ratios of Zn to Cu and Se to Cu at three time points in the two types of cancer were assessed using the generalized estimating equations (GEEs). After the patients received chemoradiotherapy for 4 weeks, SMD-2 treatment was found to be associated with a significant decrease in urinary Cu levels, whereas urinary Zn and Se levels increased significantly. In addition, the ratios of Zn to Cu and Se to Cu in the urine samples of these patients also increased significantly. Both the urinary Zn levels and the ratio of Zn to Cu in head and neck cancer patients were significantly higher than in lung cancer patients. Urinary Zn and Se levels and the ratios of Zn to Cu and Se to Cu, but not urinary Cu levels, increased significantly during and after treatment when assessed using the GEE model. The SMD-2 treatments significantly increased Zn and Se levels in the urine of head and neck cancer patients. Increased Zn and Se levels in urine strengthened immune system.

  3. Recent HIV Testing Among Young Men Who Have Sex with Men in Bangkok and Chiang Mai: HIV Testing and Prevention Strategies Must Be Enhanced in Thailand.

    PubMed

    Johnston, Lisa G; Steinhaus, Mara C; Sass, Justine; Sirinirund, Petchsri; Lee, Catherine; Benjarattanaporn, Patchara; Gass, Robert

    2016-09-01

    HIV infection among men who have sex with men, particularly in Thai urban settings and among younger cohorts, is escalating. HIV testing and counseling (HTC) are important for prevention and obtaining treatment and care. We examine data from a 2013 survey of males, 15-24 years, reporting past-year sex with a male and living in Bangkok or Chiang Mai. Almost three quarters of young MSM (YMSM) in Bangkok and only 27 % in Chiang Mai had an HIV test in the previous year. Associations for HIV testing varied between cities, although having employment increased the odds of HIV testing for both cities. In Bangkok, family knowledge of same sex attraction and talking to parents/guardians about HIV/AIDS had higher odds of HIV testing. Expanded HTC coverage is needed for YMSM in Chiang Mai. All health centers providing HTC, including those targeting MSM, need to address the specific needs of younger cohorts.

  4. Experimental basis of standardized specimen collection: the effect of moderate ethanol consumption on some serum components (K, Na, ASAT, ALAT, CK, LD, total protein).

    PubMed

    Leppänen, E A; Gräsbeck, R

    1987-06-01

    Venous blood was collected on two mornings from seven healthy volunteers using the standard Scandinavian procedure (fasting, sitting and no tourniquet) and serum Na, K, ASAT, ALAT, CK, LD and total protein were assayed. Then ethanol (0.75 g/kg body weight) was given on three consecutive evenings and the subsequent observed values 1, 3, 15, 38, 62 and 110 h post-ethanol were compared with the morning values. The mean component/total protein ratios dropped 14% for ASAT and 19% for ALAT 62 and 15 h post-ethanol, respectively. CK rose 17% at 3 h and dropped 17% at 62 h. However, the absolute values of ASAT, ALAT and CK did not change significantly. The only significant post-ethanol changes occurred in Na +2.14 mmol/l at 1 h (p less than 0.01), K -0.24 mmol/l at 3 h (p less than 0.05) and +0.26 mmol/l at 15 h (p less than 0.05), and LD +31 IU/l at 15 h (p less than 0.05) and +25 IU/l at 110 h (p less than 0.01). In one series, the total protein concentration dropped 4.57 g/l at 110 h post ethanol (p less than 0.001) but this drop was not reproducible in two additional series. It is concluded that moderate (social) ethanol consumption does not produce clinically significant effects on the components analysed. Also it may be misleading to express results as component/total protein ratios.

  5. Characterization of plant-growth-promoting effects and concurrent promotion of heavy metal accumulation in the tissues of the plants grown in the polluted soil by Burkholderia strain LD-11.

    PubMed

    Huang, Gui-Hai; Tian, Hui-Hui; Liu, Hai-Ying; Fan, Xian-Wei; Liang, Yu; Li, You-Zhi

    2013-01-01

    Plant-growth-promoting (PGP) bacteria especially with the resistance to multiple heavy metals are helpful to phytoremediation. Further development of PGP bacteria is very necessary because of the extreme diversity of plants, soils, and heavy metal pollution. A Burkholderia sp. strain, numbered LD-11, was isolated, which showed resistances to multiple heavy metals and antibiotics. It can produce indole-3-acetic acid, 1-aminocyclopropane-1-carboxylic acid deaminase and siderophores. Inoculation with the LD-11 improved germination of seeds of the investigated vegetable plants in the presence of Cu, promoted elongation of roots and hypocotyledonary axes, enhanced the dry weights of the plants grown in the soils polluted with Cu and/or Pb, and increased activity of the soil urease and the rhizobacteria diversity. Inoculation with the LD-11 significantly enhanced Cu and/or Pb accumulation especially in the roots of the plants grown in the polluted soils. Notably, LD-11 could produce siderophores in the presence of Cu. Conclusively, the PGP effects and concurrent heavy metal accumulation in the plant tissues results from combined effects of the above-mentioned multiple factors. Cu is an important element that represses production of the siderophore by the bacteria. Phytoremediation by synergistic use of the investigated plants and the bacterial strain LD-11 is a phytoextraction process.

  6. Patterns of Sexual Behavior in Lowland Thai Youth and Ethnic Minorities Attending High School in Rural Chiang Mai, Thailand

    PubMed Central

    Aurpibul, Linda; Tangmunkongvorakul, Arunrat; Musumari, Patou Masika; Srithanaviboonchai, Kriengkrai; Tarnkehard, Surapee

    2016-01-01

    Introduction The rural areas of Northern Thailand are home to a large cultural diversity of ethnic minority groups. Previous studies have shown that young people in rural Thailand have low levels of knowledge on HIV/AIDS and high sexual risks. We compared sexual behaviors between the lowland Thai youth and the youth from ethnic minority groups. Methods and findings This is a cross-sectional quantitative study conducted among high-school Thai and ethnic students in Chiang Mai. From a total 1215 participants, 487 (40.1%) were lowland Thai and 728 (59.9%) were from ethnic minorities. Overall, 17.9% of respondents reported “ever had sex.” Lowland Thai adolescents were more likely to have ever had sex compared with ethnic minority adolescents (AOR, 1.61; CI, 1.06–2.45; P< 0.01). A higher proportion of lowland Thai respondents reported having ≥ 2 lifetime sexual partners (51.9% vs. 33.3%, P = 0.003), or currently having a boy/girlfriend (59.9% vs. 45.3%, P< 0.001) compared to ethnic minority adolescents. Consistent condom use was low in both groups (22.6%). The common significant factors associated with "ever had sex" in both groups were "ever drunk alcohol in the past year" and "currently having a boy/girlfriend." Specifically, for lowland Thai youth, being around the age of 17 or 18 years and "ever used methamphetamine in the past year" were associated with increased odds of “ever had sex”. For ethnic minority adolescents, being female and belonging to religions other than Buddhism were associated with decreased odds of “ever had sex”. Conclusion A substantially higher proportion of lowland Thai engage in risky sexual behaviors when compared to ethnic minorities. However, both groups remained vulnerable to HIV and other sexually transmitted infections. To minimize sexual risks, education program and school-based interventions are warranted to increase awareness of young people about risky behaviors and to promote essential life skills. PMID:27906980

  7. “Evaluation of a peer network intervention trial among young methamphetamine users in Chiang Mai, Thailand”

    PubMed Central

    Sutcliffe, Catherine; Srirojn, Bangorn; Latkin, Carl A; Aramratanna, Ajpinun; Sherman, Susan G

    2009-01-01

    Since the 1990s, there has been a proliferation of methamphetamine use in Thailand, particularly among young people. Simultaneously, risky sexual behaviors among this population have increased. This study examined the effects of a peer network intervention and a life skills intervention on methamphetamine and HIV risk behaviors among 18–25 year olds in Chiang Mai, Thailand. Between April 2005 and June 2007, we conducted a randomized behavioral trial to compare the efficacy of a peer educator, network-oriented intervention with a best practice, life-skills curriculum on methamphetamine use, sexual behaviors, and incident sexually transmitted infections (STIs). Follow-up occurred at three, six, nine, and twelve months. Both conditions consisted of seven, two hour, small group sessions. Longitudinal analyses of the three outcomes were conducted by fitting repeated measures logistic regression models using generalized estimating equations. Participants (N=983) attended a median of six sessions, with no differences between arms. At each follow-up visit, retention was greater than 85%. Participants were 75% male and were a median of 19 years old. Over time, participants in both conditions showed a significant and dramatic decline in self-reported methamphetamine use (99% at baseline versus 53% at 12-months, p<0.0001) and significant increase in consistent condom use (32% baseline versus 44% at 12 months, p<0.0001). Incident STIs were common, with no differences between arms. Chlamydia had the highest incidence rate, 9.85/100 person-years and HIV had a low incidence rate of 0.71/100 person-years. Among young Thais, we found that a peer educator, network-oriented intervention was associated with reductions in methamphetamine use, increases in condom use, and reductions in incident STIs over 12 months. We also found parallel reductions with the life skills condition. To our knowledge, this is the first such trial targeting this population. Small group interventions are an

  8. Novel bioactive oxazolomycin isomers produced by Streptomyces albus JA3453.

    PubMed

    Kanzaki, H; Wada, K; Nitoda, T; Kawazu, K

    1998-03-01

    Two novel oxazolomycin isomers, oxazolomycins B (2) and C (3), were isolated from the fermentation broth of an oxazolomycin-producing strain, Streptomyces albus JA3453. Both compounds are geometrical isomers of oxazolomycin (1), the configurations of their triene moieties being (4'E, 6'E, 8'E) (2) and (4'Z, 6'E, 8'E) (3) while that of oxazolomycin (1) is (4'Z, 6'Z, 8'E). Compounds 2 and 3 exhibited potent inhibitory activity against crown gall formation with the same MIC (0.8 microgram/disk) as oxazolomycin. Compounds 2 and 3 showed no antibacterial activity against Agrobacterium tumefaciens, in contrast to oxazolomycin which has specific anti-A. tumefaciens activity.

  9. The Impact of Mediated Cognitive Strategies on the Reading Comprehension Performance and Self-Efficacy of Palestinian-Arab Middle School Students with LD: A Mixed-Methods Research

    ERIC Educational Resources Information Center

    Zayyad, Muhammad M.

    2009-01-01

    The purpose of this mixed-methods research was to examine the impact of mediated cognitive strategy intervention on the reading comprehension and self-efficacy of Palestinian-Arab middle school students with learning disabilities. Eighteen seventh-grade students with LD who were placed in two self-contained special education classrooms and their…

  10. High-power LD side-pump Nd: YAG regenerative amplifier at 1 kHz repetition rate with volume Bragg gratings (VBG) for broadening and compressor

    NASA Astrophysics Data System (ADS)

    Long, Ming-Liang; Chen, Li-Yuan; Chen, Meng; Li, Gang

    2016-05-01

    Pulse width of 8.7 ps was broadened to 102.2, 198 ps with single and double pass the VBG respectively. When the 102.2 ps pulse was injected into 1 kHz repetition rate of LD side-pump Nd: YAG regenerative amplifier (RA), pulse width of 87.5 ps at 1 kHz was obtained with the pulse energy of 9.4 mJ, the beam quality of M^2 factor was 1.2. The pulse width was compressed to 32.7 ps with a single pass VBG and the pulse energy reduced to 8.8 mJ, and the power density was up to 15.2 GW/cm2, the stability for pulse to pulse rms is about 0.6 %, beam pointing was about 35 μrad. In addition, when 198 ps pulse was injected into RA, pulse width of 156 ps was obtained which energy was 9.6 mJ, the pulse width was compressed to 38 ps by double passing the VBG, the pulse energy decreased to 8.5 mJ. Chirped VBG is a new way to obtain high-intensity picosecond pulse laser system simple and smaller.

  11. Mapping of PARK2 and PACRG overlapping regulatory region reveals LD structure and functional variants in association with leprosy in unrelated indian population groups.

    PubMed

    Chopra, Rupali; Ali, Shafat; Srivastava, Amit K; Aggarwal, Shweta; Kumar, Bhupender; Manvati, Siddharth; Kalaiarasan, Ponnusamy; Jena, Mamta; Garg, Vijay K; Bhattacharya, Sambit N; Bamezai, Rameshwar N K

    2013-01-01

    Leprosy is a chronic infectious disease caused by Mycobacterium Leprae, where the host genetic background plays an important role toward the disease pathogenesis. Various studies have identified a number of human genes in association with leprosy or its clinical forms. However, non-replication of results has hinted at the heterogeneity among associations between different population groups, which could be due to differently evolved LD structures and differential frequencies of SNPs within the studied regions of the genome. A need for systematic and saturated mapping of the associated regions with the disease is warranted to unravel the observed heterogeneity in different populations. Mapping of the PARK2 and PACRG gene regulatory region with 96 SNPs, with a resolution of 1 SNP per 1 Kb for PARK2 gene regulatory region in a North Indian population, showed an involvement of 11 SNPs in determining the susceptibility towards leprosy. The association was replicated in a geographically distinct and unrelated population from Orissa in eastern India. In vitro reporter assays revealed that the two significantly associated SNPs, located 63.8 kb upstream of PARK2 gene and represented in a single BIN of 8 SNPs, influenced the gene expression. A comparison of BINs between Indian and Vietnamese populations revealed differences in the BIN structures, explaining the heterogeneity and also the reason for non-replication of the associated genomic region in different populations.

  12. Mapping of PARK2 and PACRG Overlapping Regulatory Region Reveals LD Structure and Functional Variants in Association with Leprosy in Unrelated Indian Population Groups

    PubMed Central

    Chopra, Rupali; Aggarwal, Shweta; Kumar, Bhupender; Manvati, Siddharth; Kalaiarasan, Ponnusamy; Jena, Mamta; Garg, Vijay K.; Bhattacharya, Sambit N.; Bamezai, Rameshwar N. K.

    2013-01-01

    Leprosy is a chronic infectious disease caused by Mycobacterium Leprae, where the host genetic background plays an important role toward the disease pathogenesis. Various studies have identified a number of human genes in association with leprosy or its clinical forms. However, non-replication of results has hinted at the heterogeneity among associations between different population groups, which could be due to differently evolved LD structures and differential frequencies of SNPs within the studied regions of the genome. A need for systematic and saturated mapping of the associated regions with the disease is warranted to unravel the observed heterogeneity in different populations. Mapping of the PARK2 and PACRG gene regulatory region with 96 SNPs, with a resolution of 1 SNP per 1 Kb for PARK2 gene regulatory region in a North Indian population, showed an involvement of 11 SNPs in determining the susceptibility towards leprosy. The association was replicated in a geographically distinct and unrelated population from Orissa in eastern India. In vitro reporter assays revealed that the two significantly associated SNPs, located 63.8 kb upstream of PARK2 gene and represented in a single BIN of 8 SNPs, influenced the gene expression. A comparison of BINs between Indian and Vietnamese populations revealed differences in the BIN structures, explaining the heterogeneity and also the reason for non-replication of the associated genomic region in different populations. PMID:23861666

  13. A catalytic biofuel production strategy involving separate conversion of hemicellulose and cellulose using 2-sec-butylphenol (SBP) and lignin-derived (LD) alkylphenol solvents.

    PubMed

    Kim, Sunghoon; Han, Jeehoon

    2016-03-01

    A strategy in which the hemicellulose and cellulose fractions of lignocellulosic biomass are converted separately to jet fuel-range liquid hydrocarbon fuels (butene oligomers) through catalytic processes is developed. Dilute sulfuric acid (SA)-catalyzed pretreatment fractionates the first biomass into cellulose and hemicellulose-derived xylose, and these are then converted separately to levulinic acid (LA) using 2-sec-butylphenol (SBP) and lignin-derived (LD) alkylphenol solvents, respectively. LA is upgraded catalytically to butene oligomers via γ-valerolactone (GVL) and butene intermediates. Separation subsystems are designed to recover the alkylphenol solvents and biomass-derived intermediates (LA and GVL) for combination with the catalytic conversion subsystems of hemicellulose, cellulose, and lignin. In addition, a heat exchanger network (HEN) design is presented to satisfy the energy requirements of the integrated process from combustion of biomass residues (degradation products). Finally, a technoeconomic analysis shows that the proposed process ($3.37/gallon of gasoline) is an economically competitive alternative to current biofuel production approaches.

  14. Hybrid distributed Raman amplification combining random fiber laser based 2nd-order and low-noise LD based 1st-order pumping.

    PubMed

    Jia, Xin-Hong; Rao, Yun-Jiang; Yuan, Cheng-Xu; Li, Jin; Yan, Xiao-Dong; Wang, Zi-Nan; Zhang, Wei-Li; Wu, Han; Zhu, Ye-Yu; Peng, Fei

    2013-10-21

    A configuration of hybrid distributed Raman amplification (H-DRA), that is formed by incorporating a random fiber laser (RFL) based 2nd-order pump and a low-noise laser-diode (LD) based 1st-order pump, is proposed in this paper. In comparison to conventional bi-directional 1st-order DRA, the effective noise figure (ENF) is found to be lower by amount of 0 to 4 dB due to the RFL-based 2nd-order pump, depending on the on-off gain, while the low-noise 1st-order Raman pump is used for compensating the worsened signal-to-noise ratio (SNR) in the vicinity towards the far end of the fiber and avoiding the potential nonlinear impact induced by excess injection of pump power and suppressing the pump-signal relative intensity noise (RIN) transfer. As a result, the gain distribution can be optimized along ultra-long fiber link, due to combination of the 2nd-order RFL and low-noise 1st-order pumping, making the transmission distance be extended significantly. We utilized such a configuration to achieve ultra-long-distance distributed sensing based on Brillouin optical time-domain analysis (BOTDA). A repeater-less sensing distance record of up to 154.4 km with 5 m spatial resolution and ~ ± 1.4 °C temperature uncertainty is successfully demonstrated.

  15. Effects and tolerability of betahistine in patients with vestibular vertigo: results from the Romanian contingent of the OSVaLD study

    PubMed Central

    Băjenaru, Ovidiu; Roceanu, Adina Maria; Albu, Silviu; Zainea, Viorel; Pascu, Alexandru; Georgescu, Mădălina Gabriela; Cozma, Sebastian; Mărceanu, Luigi; Mureşanu, Dafin Fior

    2014-01-01

    Background and methods An efficacy population of 245 patients with vertigo of peripheral vestibular origin was recruited in Romania as part of a 3-month multinational, post-marketing surveillance study of open-label betahistine 48 mg/day (OSVaLD). Endpoints were changes in the Dizziness Handicap Index (primary endpoint), Medical Outcome Study Short-Form 36 (SF-36v2®), and the Hospital Anxiety and Depression Scale. Results During treatment, the total Dizziness Handicap Index score improved by 41 points (on a 100-point scale). Statistically significant improvements of 12–14 points were recorded in all three domains of the Dizziness Handicap Index scale (P<0.0001). Betahistine therapy was also accompanied by progressive improvements in mean Hospital Anxiety and Depression anxiety and depression scores (P<0.0001) and significant improvements in both the physical and mental component summary of the SF-36v2 (P<0.0001). Betahistine was well tolerated, with only one suspected adverse drug reaction recorded in the Romanian safety population (n=259). Conclusion Betahistine 48 mg/day was associated with improvements in multiple measures of health-related quality of life and had a good tolerability profile in these Romanian patients with recurrent peripheral vestibular vertigo. PMID:25506241

  16. THE PROGENITOR OF SN 2011ja: CLUES FROM CIRCUMSTELLAR INTERACTION

    SciTech Connect

    Chakraborti, Sayan; Ray, Alak; Yadav, Naveen; Smith, Randall; Ryder, Stuart; Sutaria, Firoza; Dwarkadas, Vikram V.; Chandra, Poonam; Pooley, David; Roy, Rupak

    2013-09-01

    Massive stars, possibly red supergiants, which retain extended hydrogen envelopes until core collapse, produce Type II plateau (IIP) supernovae. The ejecta from these explosions shocks the circumstellar matter originating from the mass loss of the progenitor during the final phases of its life. This interaction accelerates particles to relativistic energies which then lose energy via synchrotron radiation in the shock-amplified magnetic fields and inverse Compton scattering against optical photons from the supernova. These processes produce different signatures in the radio and X-ray parts of the electromagnetic spectrum. Observed together, they allow us to break the degeneracy between shock acceleration and magnetic field amplification. In this work, we use X-rays observations from the Chandra and radio observations from the Australia Telescope Compact Array to study the relative importance of processes which accelerate particles and those which amplify magnetic fields in producing the non-thermal radiation from SN 2011ja. We use radio observations to constrain the explosion date. Multiple Chandra observations allow us to probe the history of variable mass loss from the progenitor. The ejecta expands into a low-density bubble followed by interaction with a higher density wind from a red supergiant consistent with M{sub ZAMS} {approx}> 12 M{sub Sun }. Our results suggest that a fraction of Type IIP supernovae may interact with circumstellar media set up by non-steady winds.

  17. Continuous Dust Formation in SNe 2010jl and 2011ja

    NASA Astrophysics Data System (ADS)

    Krafton, Kelsie; Clayton, Geoffrey; Andrews, Jennifer; Barlow, Michael; De Looze, Ilse

    2016-08-01

    Studies in the last 10 years of dust formation in core-collapse supernovae (CCSNe) have found only small amounts, ~0.001 solar masses. This is far less than the amount needed to account for the large masses of dust seen in some high redshift galaxies. However, the recent discovery of ~1 solar mass of cold dust in the ejecta of SN 1987A has has caused a complete re-evaluation of dust formation in CCSNe. It has been suggested that the CCSNe are continuously forming dust so that by the time they are about 25 years old they will have dust masses similar to SN 1987A. However, there is a wide time gap between the CCSNe that have been studied recently and SN 1987A. We plan to use the sensitivity of Spitzer to detect dust emission from CCSNe 5 or more years after explosion. Radiative transfer models will be used to estimate the dust masses. This proposal is to continue our study of two interesting SNe 2010jl and 2011ja. These observations are part of a long term study requiring multiple epochs of Spitzer observations to look for evidence of continuous dust formation. These observations will help shed light on the mystery of dust in SN 1987A.

  18. Three-Dimensional Movements of Siachen Glacier Derived from ERS-1/2 Tandem Datasets with D-InSAR and MAI Techniques

    NASA Astrophysics Data System (ADS)

    Li, Daan; Jiang, Liming; Sun, Yongling; Wang, Hansheng

    2016-08-01

    Glacier surface velocity is one of the key para-meters of glacier dynamics and mass balance. Siachen glaciers are adversely affected due to global climate warming as reflected by these glaciers showing continuous retreat in the Karakoram. The InSAR method is a greatly valued technique to monitor the glacier flow velocity. Some previous study of Himalayan glacier velocity has been reported, but it's only the one dimensional (LOS) velocity and rare research has been made to derived the 3D velocity. The study fuse D-InSAR and MAI measurements to obtain three dimensional velocity of mountain glacier. It revealed the characteristic of glacier movement and provided reference for mass balance and climate changes. This preliminary results demonstrate the potential of integration of D-InSAR and MAI methods for estimating 3-D movements of mountain glaciers.

  19. Primary care in Brazil, and the Mais Médicos (More Doctors) Program in the Unified Health System: achievements and limits.

    PubMed

    Campos, Gastão Wagner de Sousa; Pereira, Nilton

    2016-09-01

    An historical analysis of Brazil's policies in Primary Healthcare, with emphasis on the Family Health Strategy (FHS), and the Mais Médicos Program (PMM). Studies were made of documents and secondary official data, and the bibliography that has been produced on this theme. It was found that primary healthcare has been established and successfully consolidated as an option in healthcare for a great part of the population of Brazil. There have, however, been structural hurdles, which have tended to compromise the effectiveness and sustainability of this policy. It was identified that these obstacles arise principally from insufficient financing and from inefficient modes of planning and management. The Mais Médicos Program has widened care coverage and made the distribution of primary healthcare doctors more equitable, although it has not resolved the structural problems of the public system.

  20. The Mais Médicos (More Doctors) Program: the placement of physicians in priority municipalities in Brazil from 2013 to 2014.

    PubMed

    Oliveira, João Paulo Alves; Sanchez, Mauro Niskier; Santos, Leonor Maria Pacheco

    2016-09-01

    The inadequate placement and distribution of health professionals are problems that occur in various countries. The scope of the "Programa Mais Médicos" (More Doctors Program) was to reduce the shortfall of physicians and diminish regional inequalities in health. A descriptive study on the placement of physicians between 2013 and 2014 using the Ministry of Health database is presented. There was an allocation of 14,168 physicians to the 3,785 municipalities that signed up to the program: 2,377 met the priority and/or vulnerability criteria and received 77.7% of the physicians; 1,408 received 22.3% of the physicians, but did not meet the established priorities. This study reveals the reduction in the lack of physicians, mainly in the North and Northeast. These regions account for 36% of the Brazilian population and 46.3% of the physicians were allocated there. However, the introduction of an eligibility profile, which allocated 3,166 physicians in 1,408 non-priority municipalities is questionable. The conclusion drawn is that this may have hindered the ability of the Mais Médicos Program to fully achieve its objectives as a public policy aimed at reducing regional inequalities of access to primary healthcare. Further studies are necessary to evaluate the impact of the Mais Médicos Program.

  1. Screening anaphylactic components of MaiLuoNing injection by using rat basophilic leukemia-2H3 cell membrane chromatography coupled with HPLC-ESI-TOF-MS.

    PubMed

    Han, Shengli; Lv, Yanni; Xue, Wenjing; Cao, Jiao; Cui, Ronghua; Zhang, Tao

    2016-02-01

    MaiLuoNing injection is a traditional Chinese medicine that used clinically since the 1950s in China. However, anaphylactic reactions, through the potentiation of mast cell degranulation, have been reported. In the present study, a rat basophilic leukemia-2H3 cell membrane chromatography coupled with high-performance liquid chromatography and electrospray ionization-ion trap-time of flight-mass spectrometry method was established for screening, analyzing, and identifying the potential anaphylactic components of MaiLuoNing injection. Harpagoside, a potential degranulator of rat basophilic leukemia-2H3 cells, was retained in rat basophilic leukemia-2H3 cell membrane chromatography. We aimed to evaluate the retained components to determine which of those were capable of inducing degranulation of basophilic leukemia cells. A β-hexosaminidase assay revealed that harpagoside can induce rat basophilic leukemia-2H3 cell degranulation in a dose-dependent manner. BLBA/c mice also exhibit passive cutaneous anaphylaxis in response to harpagoside. These results indicate that rat basophilic leukemia-2H3 cell membrane chromatography coupled with high-performance liquid chromatography and electrospray ionization ion trap time-of-flight mass spectrometry is effective in screening for the anaphylactic components of MaiLuoNing injection.

  2. JaK/STAT Inhibition to Prevent Post-Traumatic Epileptogenesis

    DTIC Science & Technology

    2014-09-01

    associated with epileptogenesis were determined. It was determined that upregulation of the JaK/STAT pathway in the injured hippocampus occurs after CCI...inhibitor, WP1066. Blocking JaK/STAT3 activity did not prevent loss of GABA cells in the injured hippocampus . Inhibitory postsynaptic currents in the...reduce development of post-traumatic epilepsy, and did not significantly improve memory function, but did enhance the motor recovery. These findings

  3. Late Miocene sedimentary record of the Danube/Kisalföld Basin: interregional correlation of depositional systems, stratigraphy and structural evolution

    NASA Astrophysics Data System (ADS)

    Sztanó, Orsolya; Kováč, Michal; Magyar, Imre; Šujan, Michal; Fodor, László; Uhrin, András; Rybár, Samuel; Csillag, Gábor; Tőkés, Lilla

    2016-12-01

    The Danube / Kisalföld Basin is the north-western sub-basin of the Pannonian Basin System. The lithostratigraphic subdivision of the several-km-thick Upper Miocene to Pliocene sedimentary succession related to Lake Pannon has been developed independently in Slovakia and Hungary. A study of the sedimentary formations across the entire basin led us to claim that these formations are identical or similar between the two basin parts to such an extent that their correlation is indeed a matter of nomenclature only. Nemčiňany corresponds to the Kálla Formation, representing locally derived coarse clastics along the basin margins (11- 9.5 Ma). The deep lacustrine sediments are collectively designated the Ivanka Formation in Slovakia, while in Hungary they are subdivided into Szák (fine-grained transgressive deposits above basement highs, 10.5 - 8.9 Ma), Endrőd (deep lacustrine marls, 11.6 -10 Ma), Szolnok (turbidites, 10.5 - 9.2 Ma) and Algyő Formations (fine-grained slope deposits, 10 - 9 Ma). The Beladice Formation represents shallow lacustrine deltaic deposits, fully corresponding to Újfalu (10.5 - 8.7 Ma). The overlying fluvial deposits are the Volkovce and Zagyva Formations (10 - 6 Ma). The synoptic description and characterization of these sediments offer a basin-wide insight into the development of the basin during the Late Miocene. The turbidite systems, the slope, the overlying deltaic and fluvial systems are all genetically related and are coeval at any time slice after the regression of Lake Pannon initiated about 10 Ma ago. All these formations get younger towards the S, SE as the progradation of the shelf-slope went on. The basin got filled up to lake level by 8.7 Ma, since then fluvial deposition dominated.

  4. Shared binding sites in Lepidoptera for Bacillus thuringiensis Cry1Ja and Cry1A toxins.

    PubMed

    Herrero, S; González-Cabrera, J; Tabashnik, B E; Ferré, J

    2001-12-01

    Bacillus thuringiensis toxins act by binding to specific target sites in the insect midgut epithelial membrane. The best-known mechanism of resistance to B. thuringiensis toxins is reduced binding to target sites. Because alteration of a binding site shared by several toxins may cause resistance to all of them, knowledge of which toxins share binding sites is useful for predicting cross-resistance. Conversely, cross-resistance among toxins suggests that the toxins share a binding site. At least two strains of diamondback moth (Plutella xylostella) with resistance to Cry1A toxins and reduced binding of Cry1A toxins have strong cross-resistance to Cry1Ja. Thus, we hypothesized that Cry1Ja shares binding sites with Cry1A toxins. We tested this hypothesis in six moth and butterfly species, each from a different family: Cacyreus marshalli (Lycaenidae), Lobesia botrana (Tortricidae), Manduca sexta (Sphingidae), Pectinophora gossypiella (Gelechiidae), P. xylostella (Plutellidae), and Spodoptera exigua (Noctuidae). Although the extent of competition varied among species, experiments with biotinylated Cry1Ja and radiolabeled Cry1Ac showed that Cry1Ja and Cry1Ac competed for binding sites in all six species. A recent report also indicates shared binding sites for Cry1Ja and Cry1A toxins in Heliothis virescens (Noctuidae). Thus, shared binding sites for Cry1Ja and Cry1A occur in all lepidopteran species tested so far.

  5. Update from the Japanese Center for the Validation of Alternative Methods (JaCVAM).

    PubMed

    Kojima, Hajime

    2013-12-01

    The Japanese Center for the Validation of Alternative Methods (JaCVAM) was established in 2005 to promote the use of alternatives to animal testing in regulatory studies, thereby replacing, reducing, or refining the use of animals, according to the Three Rs principles. JaCVAM assesses the utility, limitations and suitability for use in regulatory studies, of test methods needed to determine the safety of chemicals and other materials. JaCVAM also organises and performs validation studies of new test methods, when necessary. In addition, JaCVAM co-operates and collaborates with similar organisations in related fields, both in Japan and internationally, which also enables JaCVAM to provide input during the establishment of guidelines for new alternative experimental methods. These activities help facilitate application and approval processes for the manufacture and sale of pharmaceuticals, chemicals, pesticides, and other products, as well as for revisions to standards for cosmetic products. In this manner, JaCVAM plays a leadership role in the introduction of new alternative experimental methods for regulatory acceptance in Japan.

  6. Investigation of Ionospheric Disturbances Using Radio and Optical Observations in South-East Asia -- The Initial Results of the ASI and FPI Observations in Chiang Mai, Thailand

    NASA Astrophysics Data System (ADS)

    Kubota, M.; Nagatsuma, T.; Otsuka, Y.; Shiokawa, K.; Komonjinda, S.; Komolmis, T.; Somboon, E.; Tsugawa, T.; Maruyama, T.; Murata, K. T.

    2010-12-01

    For the purpose of monitoring and forecasting equatorial ionospheric disturbances, SEALION (SouthEast Asia Low-latitude IOnospheric Network) has been developed since 2003 as a cooperation project by National Institute of Information and Communications Technology (NICT), King Mongkut's Institute of Technology Ladkrabang (KMITL) in Thailand, Chiang Mai University (CMU) in Thailand, National Institute of Aeronautics and Space (LAPAN) in Indonesia, Hanoi Institute of Geophysics (HIG), Vietnamese Academy of Science and Technology in Vietnam, Center for Space Science and Applied Research (CSSAR), Chinese Academy of Sciences in China, Kyoto University in Japan, and Solar-Terrestrial Environment Laboratory (STEL), Nagoya University in Japan. SEALION consists of five ionosondes, four GPS receivers, two GPS scintillation monitors, and a magnetometer. As a part of this project, we newly installed an all-sky imager (ASI) and a Fabry-Perot Interferometer (FPI) at Sirindhorn observatory in Chiang Mai (18.8N, 98.9E, Dip lat. 13.1), Thailand. This site is located near conjugate to EAR site in Kototabang, Indonesia. One of main targets of the ASI observation is the large-scale wave structure (LSWS) with wavelengths of 100-1000 km. The LSWS is thought to be connected to the generation mechanism of equatorial plasma bubbles (EPB). The optical observations in Chiang Mai started in February 2010, and we have detected several ionospheric disturbance events with these instruments In this paper, we will show the initial results of the optical observations from Sirindhorn observatory, and discuss the features of ionospheric disturbances in Southeast Asia.

  7. Source indicators of biomass burning associated with inorganic salts and carboxylates in dry season ambient aerosol in Chiang Mai Basin, Thailand

    NASA Astrophysics Data System (ADS)

    Tsai, Ying I.; Sopajaree, Khajornsak; Chotruksa, Auranee; Wu, Hsin-Ching; Kuo, Su-Ching

    2013-10-01

    PM10 aerosol was collected between February and April 2010 at an urban site (CMU) and an industrial site (TOT) in Chiang Mai, Thailand, and characteristics and provenance of water-soluble inorganic species, carboxylates, anhydrosugars and sugar alcohols were investigated with particular reference to air quality, framed as episodic or non-episodic pollution. Sulfate, a product of secondary photochemical reactions, was the major inorganic salt in PM10, comprising 25.9% and 22.3% of inorganic species at CMU and TOT, respectively. Acetate was the most abundant monocarboxylate, followed by formate. Oxalate was the dominant dicarboxylate. A high acetate/formate mass ratio indicated that primary traffic-related and biomass-burning emissions contributed to Chiang Mai aerosols during episodic and non-episodic pollution. During episodic pollution carboxylate peaks indicated sourcing from photochemical reactions and/or directly from traffic-related and biomass burning processes and concentrations of specific biomarkers of biomass burning including water-soluble potassium, glutarate, oxalate and levoglucosan dramatically increased. Levoglucosan, the dominant anhydrosugar, was highly associated with water-soluble potassium (r = 0.75-0.79) and accounted for 93.4% and 93.7% of anhydrosugars at CMU and TOT, respectively, during episodic pollution. Moreover, levoglucosan during episodic pollution was 14.2-21.8 times non-episodic lows, showing clearly that emissions from biomass burning are the major cause of PM10 episodic pollution in Chiang Mai. Additionally, the average levoglucosan/mannosan mass ratio during episodic pollution was 14.1-14.9, higher than the 5.73-7.69 during non-episodic pollution, indicating that there was more hardwood burning during episodic pollution. Higher concentrations of glycerol and erythritol during episodic pollution further indicate that biomass burning activities released soil biota from forest and farmland soils.

  8. Expression of a Flax Allene Oxide Synthase cDNA Leads to Increased Endogenous Jasmonic Acid (JA) Levels in Transgenic Potato Plants but Not to a Corresponding Activation of JA-Responding Genes.

    PubMed Central

    Harms, K.; Atzorn, R.; Brash, A.; Kuhn, H.; Wasternack, C.; Willmitzer, L.; Pena-Cortes, H.

    1995-01-01

    Both jasmonic acid (JA) and its methyl ester, methyl jasmonate (MeJA), are thought to be significant components of the signaling pathway regulating the expression of plant defense genes in response to various stresses. JA and MeJA are plant lipid derivatives synthesized from [alpha]-linolenic acid by a lipoxygenase-mediated oxygenation leading to 13-hydroperoxylinolenic acid, which is subsequently transformed by the action of allene oxide synthase (AOS) and additional modification steps. AOS converts lipoxygenase-derived fatty acid hydroperoxide to allene epoxide, which is the precursor for JA formation. Overexpression of flax AOS cDNA under the regulation of the cauliflower mosaic virus 35S promoter in transgenic potato plants led to an increase in the endogenous level of JA. Transgenic plants had six- to 12-fold higher levels of JA than the nontransformed plants. Increased levels of JA have been observed when potato and tomato plants are mechanically wounded. Under these conditions, the proteinase inhibitor II (pin2) genes are expressed in the leaves. Despite the fact that the transgenic plants had levels of JA similar to those found in nontransgenic wounded plants, pin2 genes were not constitutively expressed in the leaves of these plants. Transgenic plants with increased levels of JA did not show changes in water state or in the expression of water stress-responsive genes. Furthermore, the transgenic plants overexpressing the flax AOS gene, and containing elevated levels of JA, responded to wounding or water stress by a further increase in JA and by activating the expression of either wound- or water stress-inducible genes. Protein gel blot analysis demonstrated that the flax-derived AOS protein accumulated in the chloroplasts of the transgenic plants. PMID:12242357

  9. Academic tutorship of the Mais Médicos para o Brasil (More Doctors to Brazil) Project in Santa Catarina: the ethical-political viewpoint.

    PubMed

    Lima, Rita de Cássia Gabrielli Souza; Gripa, Denis William; Prospero, Elisete Navas Sanches; Ros, Marco Aurélio da

    2016-09-01

    This study aims to identify the fundamentals that drive the academic tutorship of the Mais Médicos para o Brasil (More Doctors for Brazil) project in Santa Catarina, based on the unit of analysis entitled values achieved. It is an exploratory and comprehensive study carried out in 2015, with tutors of the State of Santa Catarina, decentralized reference professionals of the Education and Health Ministries, and key reference professionals of the Education Ministry. Focus groups were used as the instrument for collection of data, through semi-structured interview. Based on the category Emergency care as a force of innovation in the Mais Médicos Program, the analysis was conducted from an ethical-political approach. Three fundamentals emerged: 'Putting out fires', 'Qualification of the Project', and 'the procedural point of view'. It was concluded that academic tutorship of the productive activities of doctors in emergency care is not an isolated pedagogical approach, since it is a part and a consequence of the historic development of the theory and of the practice. The activity corresponds, dialectically, to movements of rupture, resistance, emancipation and also requires collective reflection about the values chosen and achieved in the acts of decision.

  10. Distribution and sources of polycyclic aromatic hydrocarbons in sediments of the Mai Po Inner Deep Bay Ramsar Site in Hong Kong.

    PubMed

    Zhao, Zhen-Ye; Chu, Yan-ling; Gu, Ji-Dong

    2012-08-01

    The concentration of total polycyclic aromatic hydrocarbons (∑PAHs) and the 16 US EPA priority individual PAH compounds were analyzed in surface sediments from the Mai Po Inner Deep Bay, Ramsar Site of Hong Kong from December 2001 to Jun 2005, to investigate the spatial variability of anthropogenic pollutants. ∑PAHs concentrations ranged from 36.5 to 256.3 ng g(-1) dry weight with an average of 148.9 ng g(-1), comparable to other urbanized areas of the world, and there was little difference among different sampling times from December 2001 to June 2005. Based on comparison to the results from earlier study, it appears that a decrease of total PAHs concentration has occurred since 1992. Meanwhile, the concentrations of ∑PAHs were positive correlated with total organic carbon contents except sites F and G, suggesting the characteristics of the sediment influences the distribution and concentration of PAHs. There was relatively a good relationship among the individual PAHs and the compounds of fluorene, phenanthrene, anthracene, fluoranthene, pyrene, benzo[a]pyrene and indeno[cd]pyrene yielded a good correlation (r(2) > 0.5) with total PAHs. Principal component analysis and specific PAHs compound ratios (Phe/Ant vs. Flt/Pyr) indicate the pyrogenic origins, especially traffic exhausts, are the dominant sources of PAHs in the Mai Po Inner Deep Bay Nature Reserve.

  11. Cardioprotection by combination of three compounds from ShengMai preparations in mice with myocardial ischemia/reperfusion injury through AMPK activation-mediated mitochondrial fission

    PubMed Central

    Li, Fang; Fan, Xiaoxue; Zhang, Yu; Pang, Lizhi; Ma, Xiaonan; Song, Meijia; Kou, Junping; Yu, Boyang

    2016-01-01

    GRS is a drug combination of three active components including ginsenoside Rb1, ruscogenin and schisandrin. It derived from the well-known TCM formula ShengMai preparations, a widely used traditional Chinese medicine for the treatment of cardiovascular diseases in clinic. The present study explores the cardioprotective effects of GRS on myocardial ischemia/reperfusion (MI/R) injury compared with ShengMai preparations and investigates the underlying mechanisms. GRS treatment significantly attenuated MI/R injury and exhibited similar efficacy as Shengmai preparations, as evidenced by decreased myocardium infarct size, ameliorated histological features, the decrease of LDH production and improved cardiac function, and also produced a significant decrease of apoptotic index. Mechanistically, GRS alleviated myocardial apoptosis by inhibiting the mitochondrial mediated apoptosis pathway as reflected by inhibition of caspase-3 activity, normalization of Bcl-2/Bax levels and improved mitochondrial function. Moreover, GRS prevented cardiomyocytes mitochondrial fission and upregulated AMPKα phosphorylation. Interestingly, AMPK activation prevented hypoxia and reoxygenation induced mitochondrial fission in cardiomyocytes and GRS actions were significantly attenuated by knockdown of AMPKα. Collectively, these data show that GRS is effective in mitigating MI/R injury by suppressing mitochondrial mediated apoptosis and modulating AMPK activation-mediated mitochondrial fission, thereby providing a rationale for future clinical applications and potential therapeutic strategy for MI/R injury. PMID:27869201

  12. YiQiFuMai powder injection ameliorates blood-brain barrier dysfunction and brain edema after focal cerebral ischemia-reperfusion injury in mice.

    PubMed

    Cao, Guosheng; Ye, Xinyi; Xu, Yingqiong; Yin, Mingzhu; Chen, Honglin; Kou, Junping; Yu, Boyang

    2016-01-01

    YiQiFuMai powder injection (YQFM) is a modern preparation derived from the traditional Chinese medicine Sheng-Mai-San. YQFM is widely used in clinical practice in the People's Republic of China, mainly for the treatment of microcirculatory disturbance-related diseases. However, little is known about its role in animals with ischemic stroke. The aim of this study was to examine the effect of YQFM on brain edema and blood-brain barrier (BBB) dysfunction induced by cerebral ischemia-reperfusion (I/R) injury. Male C57BL/6J mice underwent right middle cerebral artery occlusion for 1 hour with a subsequent 24-hour reperfusion to produce I/R injury. YQFM (three doses: 0.336, 0.671, and 1.342 g/kg) was then given intraperitoneally (IP). The results demonstrated that YQFM significantly decreased infarct size, improved neurological deficits, reduced brain water content, and increased cerebral blood flow after I/R injury. 18F-fluorodeoxyglucose micro-positron emission tomography imaging and hematoxylin and eosin staining results indicated that YQFM is able to ameliorate brain metabolism and histopathological damage after I/R. Moreover, YQFM administration reduced BBB leakage and upregulated the expression of zona occludens-1 (ZO-1) and occludin, which was confirmed by Evans Blue extravasation, Western blotting, and immunofluorescence assay. Our findings suggest that YQFM provides protection against focal cerebral I/R injury in mice, possibly by improving BBB dysfunction via upregulation of the expression of tight junction proteins.

  13. Optimal functional levels of activation-induced deaminase specifically require the Hsp40 DnaJa1

    PubMed Central

    Orthwein, Alexandre; Zahn, Astrid; Methot, Stephen P; Godin, David; Conticello, Silvestro G; Terada, Kazutoyo; Di Noia, Javier M

    2012-01-01

    The enzyme activation-induced deaminase (AID) deaminates deoxycytidine at the immunoglobulin genes, thereby initiating antibody affinity maturation and isotype class switching during immune responses. In contrast, off-target DNA damage caused by AID is oncogenic. Central to balancing immunity and cancer is AID regulation, including the mechanisms determining AID protein levels. We describe a specific functional interaction between AID and the Hsp40 DnaJa1, which provides insight into the function of both proteins. Although both major cytoplasmic type I Hsp40s, DnaJa1 and DnaJa2, are induced upon B-cell activation and interact with AID in vitro, only DnaJa1 overexpression increases AID levels and biological activity in cell lines. Conversely, DnaJa1, but not DnaJa2, depletion reduces AID levels, stability and isotype switching. In vivo, DnaJa1-deficient mice display compromised response to immunization, AID protein and isotype switching levels being reduced by half. Moreover, DnaJa1 farnesylation is required to maintain, and farnesyltransferase inhibition reduces, AID protein levels in B cells. Thus, DnaJa1 is a limiting factor that plays a non-redundant role in the functional stabilization of AID. PMID:22085931

  14. A balanced JA/ABA status may correlate with adaptation to osmotic stress in Vitis cells.

    PubMed

    Ismail, Ahmed; Seo, Mitsunori; Takebayashi, Yumiko; Kamiya, Yuji; Nick, Peter

    2015-08-01

    Water-related stress is considered a major type of plant stress. Osmotic stress, in particular, represents the common part of all water-related stresses. Therefore, plants have evolved different adaptive mechanisms to cope with osmotic-related disturbances. In the current work, two grapevine cell lines that differ in their osmotic adaptability, Vitis rupestris and Vitis riparia, were investigated under mannitol-induced osmotic stress. To dissect signals that lead to adaptability from those related to sensitivity, osmotic-triggered responses with respect to jasmonic acid (JA) and its active form JA-Ile, abscisic acid (ABA), and stilbene compounds, as well as the expression of their related genes were observed. In addition, the transcript levels of the cellular homeostasis gene NHX1 were examined. The data are discussed with a hypothesis suggesting that a balance of JA and ABA status might correlate with cellular responses, either guiding cells to sensitivity or to progress toward adaptation.

  15. Preliminary Results on the Mechanics of the Active Mai'iu Low Angle Normal Fault (Dayman Dome), Woodlark Rift, SE Papua New Guinea

    NASA Astrophysics Data System (ADS)

    Little, T. A.; Boulton, C. J.; Mizera, M.; Webber, S. M.; Oesterle, J.; Ellis, S. M.; Norton, K. P.; Wallace, L. M.; Biemiller, J.

    2015-12-01

    Rapid slip on the Mai'iu low-angle normal fault (LANF) has exhumed a smooth, corrugated fault surface contiguous for >24 km up-dip, rising from near sea level to ~2900 m. The fault emerges from the ground dipping ~21° N and flattens over the crest of the dome to dip south. Geomorphic analysis reveals a progressive back-tilting of the surface during exhumation accompanied by cross-cutting antithetic-sense high-angle faults—features that we attribute to "rolling-hinge" deformation of a once more steeply-dipping fault. Near the scarp base, the footwall exposes mafic mylonites that deformed at ~400-450°C. The younger Mai'iu fault cross-cuts this ductile mylonite zone, with most brittle slip being localized into a ~20 cm-thick, gouge-filled core. Near the range front, active faults bite across both the hangingwall and footwall of the Mai'iu fault and record overprinting across a dying, shallow (<~1 km deep) part of the fault by more optimally oriented, steeper faults. Such depth-dependent locking up of the fault suggests it weakens primarily by friction reduction rather than cohesion loss. Outcrop-scale fractures in the exhumed footwall reflect formation in an Andersonian stress regime. Previous campaign GPS data suggest the fault slips at up to ~1 cm/yr. To improve resolution and test for aseismic creep, we installed 12 GPS sites across the fault trace in 2015. Quantitative XRD indicates the gouges were derived primarily from mafic footwall, containing up to 65% corrensite and saponite. Hydrothermal friction experiments on two gouges from a relict LANF strand were done at varying normal stresses (30-120 MPa), temperatures (50-200oC), and sliding velocities (0.3-100 μm/s). Results reveal very weak frictional strength (μ=0.13-0.15 and 0.20-0.28) and velocity-strengthening behavior conducive to fault creep. At the highest temperatures (T≥150oC) and lowest sliding velocities (<3 μm/s), a transition to velocity-weakening behavior indicates the potential for

  16. My space, my body, my sexual subjectivity: social media, sexual practice and parental control among teenage girls in urban Chiang Mai.

    PubMed

    Fongkaew, Warunee; Fongkaew, Kangwan

    2016-01-01

    This ethnographic study conducted among young women aged 18-21 years in Chiang Mai, northern Thailand, explored the parental control mechanisms imposed by Thai middle-class families on the sexuality of their daughters. It addressed the ways in which young women tactically use the social media in order to negotiate the sexual controls they encountered in everyday life. Taking the teenage girls' point of view, this paper argues that, as active agents, young women achieve a certain level of sexual autonomy and construct their own sexual selves in modern northern Thai society, despite their parents' attempts to prevent this. The paper highlights the ways in which social media are used by Thai girls in order to achieve such a goal. Research findings should inform the development of future programmes on sexual health promotion, parental skills and sexual communication between Thai parents and their children.

  17. Prevalence of anemia, iron deficiency, thalassemia and glucose-6-phosphate dehydrogenase deficiency among hill-tribe school children in Omkoi District, Chiang Mai Province, Thailand.

    PubMed

    Yanola, Jintana; Kongpan, Chatpat; Pornprasert, Sakorn

    2014-07-01

    The prevalaence of anemia, iron deficiency, thalassemia and glucose-6-phosphate dehydrogenase (G-6-PD) deficiency were examined among 265 hill-tribe school children, 8-14 years of age, from Omkoi District, Chiang Mai Province, Thailand. Anemia was observed in 20 school children, of whom 3 had iron deficiency anemia. The prevalence of G-6-PD deficiency and β-thalassemia trait [codon 17 (A>T), IVSI-nt1 (G>T) and codons 71/72 (+A) mutations] was 4% and 8%, respectively. There was one Hb E trait, and no α-thalassemia-1 SEA or Thai type deletion. Furthermore, anemia was found to be associated with β-thalassemia trait in 11 children. These data can be useful for providing appropriate prevention and control of anemia in this region of Thailand.

  18. Distribution and characterization of methicillin-resistant Staphylococcus aureus (MRSA) at the small animal hospital, faculty of veterinary medicine, Chiang Mai University, Thailand.

    PubMed

    Patchanee, Prapas; Tadee, Pakpoom; Ingkaninan, Pimlada; Tankaew, Pallop; Hoet, Armando E; Chupia, Vena

    2014-03-01

    Of 416 samples taken from veterinary staff (n = 30), dogs (n = 356) and various environmental sites (n = 30) at the Small Animal Hospital, Faculty of Veterinary Medicine, Chiang Mai University, Thailand, 13 samples contained methicillin-resistant Staphylococcus aureus (MRSA), of which 1 (SCCmec type II) came from veterinarian, 9 (SCCmec types I, III, IVa, V and untypeable) from dogs, and 3 (SCCmec types I, III, and IVb) from environmental samples. The MRSA isolates were 100% susceptible to vancomycin (100%), 69% to cephazolin and 62% to gentamicin, but were up to 92% resistant to tetracycline group, 69% to trimethoprim-sulfamethoxazoles and 62% to ceftriaxone. In addition, all MRSA isolates showed multidrug resistance. As the MRSA isolates from the veterinary staff and dogs were of different SCCmec types, this suggests there were no cross-infections. However, environmental contamination appears to have come from dogs, and appropriate hygienic practices should be introduced to solve this problem.

  19. YiQiFuMai powder injection ameliorates blood–brain barrier dysfunction and brain edema after focal cerebral ischemia–reperfusion injury in mice

    PubMed Central

    Cao, Guosheng; Ye, Xinyi; Xu, Yingqiong; Yin, Mingzhu; Chen, Honglin; Kou, Junping; Yu, Boyang

    2016-01-01

    YiQiFuMai powder injection (YQFM) is a modern preparation derived from the traditional Chinese medicine Sheng-Mai-San. YQFM is widely used in clinical practice in the People’s Republic of China, mainly for the treatment of microcirculatory disturbance-related diseases. However, little is known about its role in animals with ischemic stroke. The aim of this study was to examine the effect of YQFM on brain edema and blood–brain barrier (BBB) dysfunction induced by cerebral ischemia–reperfusion (I/R) injury. Male C57BL/6J mice underwent right middle cerebral artery occlusion for 1 hour with a subsequent 24-hour reperfusion to produce I/R injury. YQFM (three doses: 0.336, 0.671, and 1.342 g/kg) was then given intraperitoneally (IP). The results demonstrated that YQFM significantly decreased infarct size, improved neurological deficits, reduced brain water content, and increased cerebral blood flow after I/R injury. 18F-fluorodeoxyglucose micro-positron emission tomography imaging and hematoxylin and eosin staining results indicated that YQFM is able to ameliorate brain metabolism and histopathological damage after I/R. Moreover, YQFM administration reduced BBB leakage and upregulated the expression of zona occludens-1 (ZO-1) and occludin, which was confirmed by Evans Blue extravasation, Western blotting, and immunofluorescence assay. Our findings suggest that YQFM provides protection against focal cerebral I/R injury in mice, possibly by improving BBB dysfunction via upregulation of the expression of tight junction proteins. PMID:26834461

  20. Assessment of contamination and misclassification biases in a randomized controlled trial of a social network peer education intervention to reduce HIV risk behaviors among drug users and risk partners in Philadelphia, PA and Chiang Mai, Thailand

    PubMed Central

    Simmons, Nicole; Donnell, Deborah; Ou, San-san; Celentano, David D.; Aramrattana, Apinun; Davis-Vogel, Annet; Metzger, David; Latkin, Carl

    2015-01-01

    Context Controlled trials of educational interventions are susceptible to contamination. Objectives To test a contamination measure based on recall of terms. Main study A randomized controlled trial of a social network peer education intervention among 1,123 injection drug users and risk partners in Philadelphia, PA and Chiang Mai, Thailand. Methods We assessed the recall of test, negative and positive control terms by intervention and control arm participants and compared the relative odds (OR) of recall of test vs. negative control terms between study arms. Results The contamination measure showed good discriminant ability only among participants from Chiang Mai. In Philadelphia there was no evidence of contamination and little evidence of diffusion. In Chiang Mai there was evidence of diffusion and contamination of 4 of 5 terms tested. Conclusions Network structure and peer education in Chiang Mai likely led to contamination. Recall of intervention materials can be a useful method to detect contamination in trials of educational interventions. PMID:25935214

  1. Assessment of contamination and misclassification biases in a randomized controlled trial of a social network peer education intervention to reduce HIV risk behaviors among drug users and risk partners in Philadelphia, PA and Chiang Mai, Thailand.

    PubMed

    Simmons, Nicole; Donnell, Deborah; Ou, San-San; Celentano, David D; Aramrattana, Apinun; Davis-Vogel, Annet; Metzger, David; Latkin, Carl

    2015-10-01

    Controlled trials of HIV prevention and care interventions are susceptible to contamination. In a randomized controlled trial of a social network peer education intervention among people who inject drugs and their risk partners in Philadelphia, PA and Chiang Mai, Thailand, we tested a contamination measure based on recall of intervention terms. We assessed the recall of test, negative and positive control terms among intervention and control arm participants and compared the relative odds of recall of test versus negative control terms between study arms. The contamination measures showed good discriminant ability among participants in Chiang Mai. In Philadelphia there was no evidence of contamination and little evidence of diffusion. In Chiang Mai there was strong evidence of diffusion and contamination. Network structure and peer education in Chiang Mai likely led to contamination. Recall of intervention materials can be a useful method to detect contamination in experimental interventions.

  2. Integrated metabolomic and proteomic analysis reveals systemic responses of Rubrivivax benzoatilyticus JA2 to aniline stress.

    PubMed

    Mujahid, Md; Prasuna, M Lakshmi; Sasikala, Ch; Ramana, Ch Venkata

    2015-02-06

    Aromatic amines are widely distributed in the environment and are major environmental pollutants. Although degradation of aromatic amines is well studied in bacteria, physiological adaptations and stress response to these toxic compounds is not yet fully understood. In the present study, systemic responses of Rubrivivax benzoatilyticus JA2 to aniline stress were deciphered using metabolite and iTRAQ-labeled protein profiling. Strain JA2 tolerated high concentrations of aniline (30 mM) with trace amounts of aniline being transformed to acetanilide. GC-MS metabolite profiling revealed aniline stress phenotype wherein amino acid, carbohydrate, fatty acid, nitrogen metabolisms, and TCA (tricarboxylic acid cycle) were modulated. Strain JA2 responded to aniline by remodeling the proteome, and cellular functions, such as signaling, transcription, translation, stress tolerance, transport and carbohydrate metabolism, were highly modulated. Key adaptive responses, such as transcription/translational changes, molecular chaperones to control protein folding, and efflux pumps implicated in solvent extrusion, were induced in response to aniline stress. Proteo-metabolomics indicated extensive rewiring of metabolism to aniline. TCA cycle and amino acid catabolism were down-regulated while gluconeogenesis and pentose phosphate pathways were up-regulated, leading to the synthesis of extracellular polymeric substances. Furthermore, increased saturated fatty acid ratios in membranes due to aniline stress suggest membrane adaptation. The present study thus indicates that strain JA2 employs multilayered responses: stress response, toxic compound tolerance, energy conservation, and metabolic rearrangements to aniline.

  3. Transcriptome Analysis in Haematococcus pluvialis: Astaxanthin Induction by Salicylic Acid (SA) and Jasmonic Acid (JA).

    PubMed

    Gao, Zhengquan; Li, Yan; Wu, Guanxun; Li, Guoqiang; Sun, Haifeng; Deng, Suzhen; Shen, Yicheng; Chen, Guoqiang; Zhang, Ruihao; Meng, Chunxiao; Zhang, Xiaowen

    2015-01-01

    Haematococcus pluvialis is an astaxanthin-rich microalga that can increase its astaxanthin production by salicylic acid (SA) or jasmonic acid (JA) induction. The genetic transcriptome details of astaxanthin biosynthesis were analyzed by exposing the algal cells to 25 mg/L of SA and JA for 1, 6 and 24 hours, plus to the control (no stress). Based on the RNA-seq analysis, 56,077 unigenes (51.7%) were identified with functions in response to the hormone stress. The top five identified subcategories were cell, cellular process, intracellular, catalytic activity and cytoplasm, which possessed 5600 (~9.99%), 5302 (~9.45%), 5242 (~9.35%), 4407 (~7.86%) and 4195 (~7.48%) unigenes, respectively. Furthermore, 59 unigenes were identified and assigned to 26 putative transcription factors (TFs), including 12 plant-specific TFs. They were likely associated with astaxanthin biosynthesis in Haematococcus upon SA and JA stress. In comparison, the up-regulation of differential expressed genes occurred much earlier, with higher transcript levels in the JA treatment (about 6 h later) than in the SA treatment (beyond 24 h). These results provide valuable information for directing metabolic engineering efforts to improve astaxanthin biosynthesis in H. pluvialis.

  4. Partial Activation of SA- and JA-Defensive Pathways in Strawberry upon Colletotrichum acutatum Interaction

    PubMed Central

    Amil-Ruiz, Francisco; Garrido-Gala, José; Gadea, José; Blanco-Portales, Rosario; Muñoz-Mérida, Antonio; Trelles, Oswaldo; de los Santos, Berta; Arroyo, Francisco T.; Aguado-Puig, Ana; Romero, Fernando; Mercado, José-Ángel; Pliego-Alfaro, Fernando; Muñoz-Blanco, Juan; Caballero, José L.

    2016-01-01

    Understanding the nature of pathogen host interaction may help improve strawberry (Fragaria × ananassa) cultivars. Plant resistance to pathogenic agents usually operates through a complex network of defense mechanisms mediated by a diverse array of signaling molecules. In strawberry, resistance to a variety of pathogens has been reported to be mostly polygenic and quantitatively inherited, making it difficult to associate molecular markers with disease resistance genes. Colletotrichum acutatum spp. is a major strawberry pathogen, and completely resistant cultivars have not been reported. Moreover, strawberry defense network components and mechanisms remain largely unknown and poorly understood. Assessment of the strawberry response to C. acutatum included a global transcript analysis, and acidic hormones SA and JA measurements were analyzed after challenge with the pathogen. Induction of transcripts corresponding to the SA and JA signaling pathways and key genes controlling major steps within these defense pathways was detected. Accordingly, SA and JA accumulated in strawberry after infection. Contrastingly, induction of several important SA, JA, and oxidative stress-responsive defense genes, including FaPR1-1, FaLOX2, FaJAR1, FaPDF1, and FaGST1, was not detected, which suggests that specific branches in these defense pathways (those leading to FaPR1-2, FaPR2-1, FaPR2-2, FaAOS, FaPR5, and FaPR10) were activated. Our results reveal that specific aspects in SA and JA dependent signaling pathways are activated in strawberry upon interaction with C. acutatum. Certain described defense-associated transcripts related to these two known signaling pathways do not increase in abundance following infection. This finding suggests new insight into a specific putative molecular strategy for defense against this pathogen. PMID:27471515

  5. Partial Activation of SA- and JA-Defensive Pathways in Strawberry upon Colletotrichum acutatum Interaction.

    PubMed

    Amil-Ruiz, Francisco; Garrido-Gala, José; Gadea, José; Blanco-Portales, Rosario; Muñoz-Mérida, Antonio; Trelles, Oswaldo; de Los Santos, Berta; Arroyo, Francisco T; Aguado-Puig, Ana; Romero, Fernando; Mercado, José-Ángel; Pliego-Alfaro, Fernando; Muñoz-Blanco, Juan; Caballero, José L

    2016-01-01

    Understanding the nature of pathogen host interaction may help improve strawberry (Fragaria × ananassa) cultivars. Plant resistance to pathogenic agents usually operates through a complex network of defense mechanisms mediated by a diverse array of signaling molecules. In strawberry, resistance to a variety of pathogens has been reported to be mostly polygenic and quantitatively inherited, making it difficult to associate molecular markers with disease resistance genes. Colletotrichum acutatum spp. is a major strawberry pathogen, and completely resistant cultivars have not been reported. Moreover, strawberry defense network components and mechanisms remain largely unknown and poorly understood. Assessment of the strawberry response to C. acutatum included a global transcript analysis, and acidic hormones SA and JA measurements were analyzed after challenge with the pathogen. Induction of transcripts corresponding to the SA and JA signaling pathways and key genes controlling major steps within these defense pathways was detected. Accordingly, SA and JA accumulated in strawberry after infection. Contrastingly, induction of several important SA, JA, and oxidative stress-responsive defense genes, including FaPR1-1, FaLOX2, FaJAR1, FaPDF1, and FaGST1, was not detected, which suggests that specific branches in these defense pathways (those leading to FaPR1-2, FaPR2-1, FaPR2-2, FaAOS, FaPR5, and FaPR10) were activated. Our results reveal that specific aspects in SA and JA dependent signaling pathways are activated in strawberry upon interaction with C. acutatum. Certain described defense-associated transcripts related to these two known signaling pathways do not increase in abundance following infection. This finding suggests new insight into a specific putative molecular strategy for defense against this pathogen.

  6. Prevalence and Correlates of HIV Testing among Young People Enrolled in Non-Formal Education Centers in Urban Chiang Mai, Thailand: A Cross-Sectional Study

    PubMed Central

    Musumari, Patou Masika; Tangmunkongvorakul, Arunrat; Srithanaviboonchai, Kriengkrai; Yungyuankul, Sawang; Techasrivichien, Teeranee; Suguimoto, S. Pilar; Ono-Kihara, Masako; Kihara, Masahiro; Chariyalertsak, Suwat

    2016-01-01

    Background HIV testing is the gateway to HIV prevention, treatment, and care. Despite the established vulnerability of young Thai people to HIV infection, studies examining the prevalence and correlates of HIV testing among the general population of Thai youth are still very limited. This study investigates socio-demographic, behavioral, and psychosocial factors associated with HIV testing among young Thai people enrolled in Non-formal Education Centers (NFEC) in urban Chiang Mai, Northern Thailand. Methods This was a cross-sectional quantitative study conducted among young unmarried Thai youth—between the ages of 15 and 24—who were enrolled in NFEC in urban Chiang Mai. Multiple logistic regressions were used to identify correlates of “ever tested for HIV” among the sexually active participants. Findings Of the 295 sexually active participants, 27.3% reported “ever tested for HIV;” 65.4% “did not consistently use condom;” and 61.7% “had at least 2 lifetime partners.” We found that “self-efficacy” (AOR, 4.92; CI, 1.22–19.73); “perception that it is easy to find a location nearby to test for HIV” (AOR, 4.67; CI, 1.21–18.06); “having at least 2 lifetime sexual partners” (AOR, 2.05; CI, 1.09–3.85); and “ever been pregnant or made someone pregnant” (AOR, 4.06; CI, 2.69–9.15); were associated with increased odds of having ever been tested. On the other hand, “fear of HIV test results” (AOR, 0.21; CI, 0.08–0.57) was associated with lower odds of ever having been tested for HIV. Conclusion The main finding is that a substantially high proportion of Thai youth is engaged in risky sexual behaviors—yet reports low rates of ever having been tested for HIV. This highlights an urgent need to develop appropriate interventions—based on the identified correlates of HIV testing. There is also an urgent need to enhance HIV testing and to promote safer sexual behaviors among young Thai people—particularly those who are out

  7. Estimation of gas-particle partitioning coefficients (Kp) of carcinogenic polycyclic aromatic hydrocarbons in carbonaceous aerosols collected at Chiang-Mai, Bangkok and Hat-Yai, Thailand.

    PubMed

    Pongpiachan, Siwatt; Ho, Kin Fai; Cao, Junji

    2013-01-01

    To assess environmental contamination with carcinogens, carbonaceous compounds, water-soluble ionic species and trace gaseous species were identified and quantified every three hours for three days at three different atmospheric layers at the heart of Chiang-Mai, Bangkok and Hat-Yai from December 2006 to February 2007. A DRI Model 2001 Thermal/Optical Carbon Analyzer with the IMPROVE thermal/optical reflectance (TOR) protocol was used to quantify the organic carbon (OC) and elemental carbon (EC) contents in PM10. Diurnal and vertical variability was also carefully investigated. In general, OC and EC mass concentration showed the highest values at the monitoring period of 21.00-00.00 as consequences of human activities at night bazaar coupled with reduction of mixing layer, decreased wind speed and termination of photolysis at nighttime. Morning peaks of carbonaceous compounds were observed during the sampling period of 06:00-09:00, emphasizing the main contribution of traffic emission in the three cities. The estimation of incremental lifetime particulate matter exposure (ILPE) raises concern of high risk of carbonaceous accumulation over workers and residents living close to the observatory sites. The average values of incremental lifetime particulate matter exposure (ILPE) of total carbon at Baiyoke Suit Hotel and Baiyoke Sky Hotel are approximately ten times higher than those air samples collected at Prince of Songkla University Hat-Yai campus corpse incinerator and fish-can manufacturing factory but only slightly higher than those of rice straw burning in Songkla province. This indicates a high risk of developing lung cancer and other respiratory diseases across workers and residents living in high buildings located in Pratunam area. Using knowledge of carbonaceous fractions in PM10, one can estimate the gas-particle partitioning of polycyclic aromatic hydrocarbons (PAHs). Dachs-Eisenreich model highlights the crucial role of adsorption in gas

  8. Physiological Characteristics and Production of Folic Acid of Lactobacillus plantarum JA71 Isolated from Jeotgal, a Traditional Korean Fermented Seafood

    PubMed Central

    Lim, Sang-Dong

    2014-01-01

    Folic acid, one of the B group of vitamins, is an essential substance for maintaining the functions of the nervous system, and is also known to decrease the level of homocysteine in plasma. Homocysteine influences the lowering of the cognitive function in humans, and especially in elderly people. In order to determine the strains with a strong capacity to produce folic acid, 190 bacteria were isolated from various kinds of jeotgal and chungkuk-jang. In our test experiment, JA71 was found to contain 9.03μg/mL of folic acid after 24 h of incubation in an MRS broth. This showed that JA71 has the highest folic acid production ability compared to the other lactic acid bacteria that were isolated. JA71 was identified as Lactobacillus plantarum by the result of API carbohydrate fermentation pattern and 16s rDNA sequence. JA71 was investigated for its physiological characteristics. The optimum growth temperature of JA71 was 37℃, and the cultures took 12 h to reach pH 4.4. JA71 proved more sensitive to bacitracin when compared with fifteen different antibiotics, and showed most resistance to neomycin and vancomycin. Moreover, it was comparatively tolerant of bile juice and acid, and displayed resistance to Escherichia coli, Salmonella Typhimurium, and Staphylococcus aureus with restraint rates of 60.4%, 96.7%, and 76.2%, respectively. These results demonstrate that JA71 could be an excellent strain for application to functional products. PMID:26760752

  9. Field evaluation of G10, a celery (Apium graveolens)-based topical repellent, against mosquitoes (Diptera: Culicidae) in Chiang Mai province, northern Thailand.

    PubMed

    Tuetun, B; Choochote, W; Pongpaibul, Y; Junkum, A; Kanjanapothi, D; Chaithong, U; Jitpakdi, A; Riyong, D; Wannasan, A; Pitasawat, B

    2009-02-01

    The potential of G10, a celery (Apium graveolens)-based topical product, as a repellent against natural mosquito populations was evaluated in comparison to commercial (Insect Block 28) and standard (25% DEET) repellents in Chiang Mai province, northern Thailand. These repellent products afforded encouragingly excellent personal protection against a broad range of mosquito species belonging to various genera, including Aedes, Anopheles, Armigeres, Culex, and Mansonia. No mosquito bite was observed on the volunteers treated with G10 and Insect Block 28 throughout the field study, whereas two species, i.e., six A. barbirostris and two A. subalbatus, came to bite or land on 25% DEET-treated volunteers. Thus, it can be concluded that while G10 and Insect Block 28 exhibited similarly powerful repellent activities with complete (100%) protection, 25% DEET was effective in minimizing bites with 99.68% protection. G10 formula was also studied for physical properties and biological stability after being kept under two conditions; a heating and cooling cycle, and varying temperature and time storage. Most samples of stored G10 not only demonstrated a similarity in appearance and physical properties, but also provided comparable repellency to that of the fresh preparation. These findings encourage commercial development of G10 formula as an alternative to conventional synthetic repellents.

  10. Protective effect of ultrafiltered XinMaiJia extract against H₂O₂-induced injury in human umbilical vein endothelial cells through NHE1 downregulation.

    PubMed

    Yin, Y L; Li, P; Yang, J; Liu, D W; Sun, R L; Pan, G P; Wan, G M; Wan, G R

    2014-10-20

    We examined the protective effects of ultrafiltered XinMaiJia (XMJ) extract in a hydrogen peroxide (H2O2)-induced injury model in human umbilical vein endothelial cells (HUVECs) and determined the corresponding changes in the Na(+)-H(+) exchanger (NHE1) protein content and NHE1 gene expression. H2O2-induced HUVECs were treated with different concentrations of XMJ extract and the corresponding changes in morphology, activity, membrane permeability, biochemical indicators, cytokine concentration, NHE1 protein content, and NHE1 gene expression were determined. H2O2 significantly promoted HUVEC injury, whereas ultrafiltered XMJ extract significantly improved the morphological changes in injured HUVECs, increased their activity, and decreased NHE1 gene and protein expression, as well as limited the decrease in membrane permeability and expression of intercellular adhesion molecule-1, vascular cell adhesion molecule-1, interleukin (IL)-1, IL-6, and nuclear factor-kB. Ultrafiltered XMJ extract inhibited H2O2-induced HUVEC injury by inhibiting NHE1 activity.

  11. Participation of small municipalities in the Mais Médicos para o Brasil (More Doctors to Brazil) Program in the macro-region of Northern Paraná.

    PubMed

    Mendonça, Fernanda de Freitas; Mattos, Luis Fernando Abucarub de; Oliveira, Emmeline Bernardes Duarte de; Domingos, Carolina Milena; Okamura, Carlos Takeo; Carvalho, Brígida Gimenez; Nunes, Elisabete de Fátima Polo de Almeida

    2016-09-01

    Doctor shortage is a constant problem in smaller cities and towns, which tend to be more vulnerable from a social and economic point of view, and located in geographically isolated areas. The goal of this study was to establish the share and characteristics of the small cities and towns in the macro-region of northern Paraná that subscribed to the Mais Médicos para o Brasil (PMMB) program. This is a quantitative study of 82 cities and towns using primary and secondary data. Results show that only a few of them (6.1%) had any adherence criteria, which was not an impediment for other cities and towns (75%) to adhere to the project. Cities and towns with over five thousand inhabitants, lower municipal HDI (Human Development Index), some adherence criteria and more geographically isolated, either from the main city in the region or the closest large or mid-sized city, tended to adhere to the PMMB. It is undeniable that the PMMB significantly reduced the uneven distribution of doctors in Brazil and the study region. However, the sustainability of this policy is linked to addressing other remaining challenges in the SUS system.

  12. Monitoring and evaluation of the PAHO/WHO cooperation project, the Mais Médicos (More Doctors) Program: a mid-term assessment.

    PubMed

    Molina, Joaquín; Tasca, Renato; Suárez, Julio

    2016-09-01

    Working relations between the Pan- American Health Organization/World Health Organization (PAHO/WHO) and Brazilian health institutions accumulated a long history of cooperation with mutual benefits, which in many cases were shared with other nations under various cooperation frameworks among countries for health development. A milestone in this relationship is the technical cooperation provided by PAHO/WHO to the More Doctors Program (Programa Mais Médicos - PMM). This cooperation has added both strategic value in reducing gaps in health equality and has capitalized on the unique nature of the Cuba-Brazil South-South cooperation experience, triangulated through PAHO/WHO. This paper discusses PAHO/WHO's role in the evaluation of its technical cooperation within PMM. A Monitoring and Evaluation (M&E) Framework has been developed in order to progressively identify the advances in coverage and quality of primary health care provided by the Unified Health System (Sistema Único de Saúde - SUS) through the PMM. Special attention was given to identify best practices in health services, to analyze results and impacts of the PMM, and to manage and share knowledge that has been produced by its implementation, through a web-based knowledge platform. Some relevant results of PMM are briefly presented and discussed.

  13. Effects of Herbal Medicine (Gan Mai Da Zao Decoction) on Several Types of Neuropsychiatric Disorders in an Animal Model: A Systematic Review

    PubMed Central

    Kim, Su Ran; Lee, Hye Won; Jun, Ji Hee; Ko, Byoung-Seob

    2017-01-01

    Objectives: Gan Mai Da Zao (GMDZ) decoction is widely used for the treatment of various diseases of the internal organ and of the central nervous system. The aim of this study is to investigate the effects of GMDZ decoction on neuropsychiatric disorders in an animal model. Methods: We searched seven databases for randomized animal studies published until April 2015: Pubmed, four Korean databases (DBpia, Oriental Medicine Advanced Searching Integrated System, Korean Studies Information Service System, and Research Information Sharing Service), and one Chinese database (China National Knowledge Infrastructure). The randomized animal studies were included if the effects of GMDZ decoction were tested on neuropsychiatric disorders. All articles were read in full and extracted predefined criteria by two independent reviewers. Results: From a total of 258 hits, six randomized controlled animal studies were included. Five studies used a Sprague Dawley rat model for acute psychological stress, post-traumatic stress disorders, and unpredictable mild stress depression whereas one study used a Kunming mouse model for prenatal depression. The results of the studies showed that GMDZ decoction improved the related outcomes. Conclusion: Regardless of the dose and concentration used, GMDZ decoction significantly improved neuropsychiatric disease-related outcomes in animal models. However, additional systematic and extensive studies should be conducted to establish a strong conclusion. PMID:28392956

  14. Assessment of the scope of practice of physicians participating in the Mais Médicos (More Doctors) Program, and associated factors.

    PubMed

    Girardi, Sábado Nicolau; Carvalho, Cristiana Leite; Pierantoni, Célia Regina; Costa, Juliana de Oliveira; Stralen, Ana Cristina de Sousa van; Lauar, Thaís Viana; David, Renata Bernardes

    2016-09-01

    The purpose of this study was to characterize the scope of practice of physicians working in primary healthcare participating in the Mais Médicos (More Doctors) Program ('PMM') and investigate the factors associated with execution of a larger number of clinical activities. It is an exploratory study carried over January to March 2016, through a self-applied questionnaire containing a list of 49 procedures, activities and actions carried out in primary healthcare. A total of 1,241 physicians took part in the study, most of them female, between age 40 and 49, and of Cuban nationality. The physicians carried out an average of 22.8 ± 8.2 procedures; they reported knowing how to carry out a larger number of procedures. Factors associated with executing a larger number of procedures were: being male, having graduated more recently, two years or less practicing in their primary healthcare unit, practicing in the North or South geographical regions, in small towns and more distant from the regional health headquarters. The main reason for not carrying out the procedures and activities that they reported knowing how to do was the lack of materials and inadequate infrastructure. The results show that the scope of practice of the physicians of the PMM is lower than their capacities, and that interventions with the intention of widening their scope are necessary.

  15. Quantification of contamination levels and particular risk of Salmonella spp. in pigs in slaughterhouses in Chiang Mai and Lamphun provinces, Thailand.

    PubMed

    Tadee, Pakpoom; Boonkhot, Phacharaporn; Patchanee, Prapas

    2014-11-01

    Salmonella spp. is one of the important foodborne pathogens, and the slaughtering process is recognized as a potential point of contamination and the spread of the pathogens. The three objectives of this study are first, to quantify the contamination levels of Salmonella spp. in pig skins and carcasses, second, to evaluate the outcomes from different pig supply sources and different practices at three critical steps (scalding, splitting, and washing) for Salmonella spp. contamination, and third, to assess risk of Salmonella spp. contamination in pork products after slaughtering level. The study was performed in three representative slaughterhouses in Chiang Mai and Lamphun provinces, Thailand. Investigation conducted from May 2013 through October 2013 found the overall prevalence and contamination levels mean to be 11.85% and 0.34 MPN/cm2, respectively. There was no statistically significant in Salmonella spp. prevalence and contamination levels detected with different patterns at the slaughterhouses which were supplied pigs from either co-operative or integrated farms. Factors found to reduce Salmonella spp. loads on carcasses included good practices, e.g., regular changing of water in the scalding tank after each batch and the use of chlorine in the washing step. Risk of Salmonella spp. contamination of pork products at the final stage of slaughtering was nearly 10%. Good practices and proper hygiene measures should be applied to minimize the risk of Salmonella spp. exposure in the slaughtering line, which can reduce the contamination pressure downstream at retail shops as well as for end consumers.

  16. Protective effect of the ultra-filtration extract from Xin Mai Jia on human aortic smooth muscle cell injury induced by hydrogen peroxide

    PubMed Central

    WAN, JIA; YIN, YALING; SUN, RUILI; PAN, GUOPIN; LI, PENG; JIA, YANLONG; WAN, GUANGRUI; LIU, ZHANG-SUO

    2014-01-01

    The aim of the present study was to explore whether an ultra-filtration extract from Xin Mai Jia (XMJ), a Chinese medicinal formulation, has a protective effect on human aortic smooth muscle cell (HASMC) injury models induced by hydrogen peroxide (H2O2), and to consider the mechanism and efficacy of the therapeutic action of XMJ on atherosclerosis. HASMCs were injured by H2O2 and then exposed to various concentrations of XMJ. The morphological changes, growth, proliferation, migration and cytokine release of HASMCs were detected using 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT), an enzyme-linked immunosorbent assay and a scratch adhesion test. H2O2 significantly promoted the proliferation of HASMCs. The ultra-filtration extract from XMJ was observed to significantly attenuate the morphological changes of injured HASMCs, reduce the expression levels of intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), interleukin (IL)-1, IL-6 and nuclear factor (NF)-κB, and increase the expression levels of matrix metalloproteinase (MMP)-2 and tissue inhibitor of metalloproteinase (TIMP). XMJ has clear anti-inflammatory and antioxidant effects, and significantly inhibits the proliferation and migration of HASMCs. PMID:24348756

  17. New Enhanced Artificial Bee Colony (JA-ABC5) Algorithm with Application for Reactive Power Optimization

    PubMed Central

    2015-01-01

    The standard artificial bee colony (ABC) algorithm involves exploration and exploitation processes which need to be balanced for enhanced performance. This paper proposes a new modified ABC algorithm named JA-ABC5 to enhance convergence speed and improve the ability to reach the global optimum by balancing exploration and exploitation processes. New stages have been proposed at the earlier stages of the algorithm to increase the exploitation process. Besides that, modified mutation equations have also been introduced in the employed and onlooker-bees phases to balance the two processes. The performance of JA-ABC5 has been analyzed on 27 commonly used benchmark functions and tested to optimize the reactive power optimization problem. The performance results have clearly shown that the newly proposed algorithm has outperformed other compared algorithms in terms of convergence speed and global optimum achievement. PMID:25879054

  18. Aniline is an inducer, and not a precursor, for indole derivatives in Rubrivivax benzoatilyticus JA2.

    PubMed

    Mujahid, Mohammed; Sasikala, Ch; Ramana, Ch V

    2014-01-01

    Rubrivivax benzoatilyticus JA2 and other anoxygenic photosynthetic bacteria produce indole derivatives when exposed to aniline, a xenobiotic compound. Though this phenomenon has been reported previously, the role of aniline in the production of indoles is still a biochemical riddle. The present study aims at understanding the specific role of aniline (as precursor or stimulator) in the production of indoles and elucidating the biochemical pathway of indoles in aniline-exposed cells by using stable isotope approaches. Metabolic profiling revealed tryptophan accumulation only in aniline exposed cells along with indole 3-acetic acid (IAA) and indole 3-aldehyde (IAld), the two major catabolites of tryptophan. Deuterium labelled aniline feeding studies revealed that aniline is not a precursor of indoles in strain JA2. Further, production of indoles only in aniline-exposed cells suggests that aniline is an indoles stimulator. In addition, production of indoles depended on the presence of a carbon source, and production enhanced when carbon sources were added to the culture. Isotope labelled fumarate feeding identified, fumarate as the precursor of indole, indicating de novo synthesis of indoles. Glyphosate (shikimate pathway inhibitor) inhibited the indoles production, accumulation of tryptophan, IAA and IAld indicating that indoles synthesis in strain JA2 occurs via the de novo shikimate pathway. The up-regulation of anthranilate synthase gene and induction of anthranilate synthase activity correlated well with tryptophan production in strain JA2. Induction of tryptophan aminotransferase and tryptophan 2-monooxygenase activities corroborated well with IAA levels, suggesting that tryptophan catabolism occurs simultaneously in aniline exposed cells. Our study demonstrates that aniline (stress) stimulates tryptophan/indoles synthesis via the shikimate pathway by possibly modulating the metabolic pathway.

  19. Aniline Is an Inducer, and Not a Precursor, for Indole Derivatives in Rubrivivax benzoatilyticus JA2

    PubMed Central

    Mohammed, Mujahid; Ch, Sasikala; Ch, Ramana V.

    2014-01-01

    Rubrivivax benzoatilyticus JA2 and other anoxygenic photosynthetic bacteria produce indole derivatives when exposed to aniline, a xenobiotic compound. Though this phenomenon has been reported previously, the role of aniline in the production of indoles is still a biochemical riddle. The present study aims at understanding the specific role of aniline (as precursor or stimulator) in the production of indoles and elucidating the biochemical pathway of indoles in aniline-exposed cells by using stable isotope approaches. Metabolic profiling revealed tryptophan accumulation only in aniline exposed cells along with indole 3-acetic acid (IAA) and indole 3-aldehyde (IAld), the two major catabolites of tryptophan. Deuterium labelled aniline feeding studies revealed that aniline is not a precursor of indoles in strain JA2. Further, production of indoles only in aniline-exposed cells suggests that aniline is an indoles stimulator. In addition, production of indoles depended on the presence of a carbon source, and production enhanced when carbon sources were added to the culture. Isotope labelled fumarate feeding identified, fumarate as the precursor of indole, indicating de novo synthesis of indoles. Glyphosate (shikimate pathway inhibitor) inhibited the indoles production, accumulation of tryptophan, IAA and IAld indicating that indoles synthesis in strain JA2 occurs via the de novo shikimate pathway. The up-regulation of anthranilate synthase gene and induction of anthranilate synthase activity correlated well with tryptophan production in strain JA2. Induction of tryptophan aminotransferase and tryptophan 2-monooxygenase activities corroborated well with IAA levels, suggesting that tryptophan catabolism occurs simultaneously in aniline exposed cells. Our study demonstrates that aniline (stress) stimulates tryptophan/indoles synthesis via the shikimate pathway by possibly modulating the metabolic pathway. PMID:24533057

  20. University Cooperation Platform (UCP) between Christian-Albrechts-University Kiel (Germany) and Chiang Mai University (Thailand): implementation of image-guided gynecological brachytherapy.

    PubMed

    Galalae, Razvan; Tharavichitkul, Ekkasit; Wanwilairat, Somsak; Chitapanarux, Imjai; Kimmig, Bernhard; Dunst, Jürgen; Lorvidhaya, Vicharn

    2015-02-01

    Starting in 1999, the University Cooperation Platform (UCP) implemented an exchange program of researchers and clinicians/physicists between the Christian-Albrechts-University Kiel in Germany and Chiang Mai University in Thailand, to initiate a sustainable base for long-term development of image-guided brachytherapy and in general for high-technology radiotherapy in Chiang Mai. A series of UCP protocols, based constructively on each other, were performed and evaluated at intermediate term follow-up. The first protocol, addressing computed tomography (CT)-optimized brachytherapy for advanced cervical cancer (n = 17), showed a significant reduction of D2cc for the bladder and sigmoid (p < 0.001) while maintaining a very high dose in D90 high-risk clinical target volume (HR-CTV) in comparison with standard point-based planning. In addition, after a follow-up of 19 months no tumor relapse was observed. The second UCP protocol, testing the impact of magnetic resonance imaging (MRI) guidance (n = 15) in patients with cervical cancer, proved significantly smaller D2cc doses for the bladder, rectum, and sigmoid (p = 0.003, p = 0.015, and p = 0.012), and secured highly curative mean doses in D90 HR-CTV of 99.2 Gy. The acute and late toxicity was excellent without any observed grade 3 or higher morbidity. In the third protocol, the combination of image-guided brachytherapy (IGBT) and whole pelvis intensity-modulated external beam radiotherapy (WP-IMRT) (n = 15) reaffirmed the significant reduction of D2cc doses for the bladder, rectum, and sigmoid (p = 0.001 or p < 0.001) along with high equivalent dose at 2 Gy (EQD2) in the HR-CTV, and demonstrated very low acute therapy-related toxicity in absence of grade 3 morbidity. The implementation of transabdominal ultrasound (TAUS) was the focus of the fourth UCP project aiming a more generous potential use of image-guidance on long-term, and enhancing the quality of soft tissue assessment complementary to conventionally planned

  1. University Cooperation Platform (UCP) between Christian-Albrechts-University Kiel (Germany) and Chiang Mai University (Thailand): implementation of image-guided gynecological brachytherapy

    PubMed Central

    Tharavichitkul, Ekkasit; Wanwilairat, Somsak; Chitapanarux, Imjai; Kimmig, Bernhard; Dunst, Jürgen; Lorvidhaya, Vicharn

    2015-01-01

    Starting in 1999, the University Cooperation Platform (UCP) implemented an exchange program of researchers and clinicians/physicists between the Christian-Albrechts-University Kiel in Germany and Chiang Mai University in Thailand, to initiate a sustainable base for long-term development of image-guided brachytherapy and in general for high-technology radiotherapy in Chiang Mai. A series of UCP protocols, based constructively on each other, were performed and evaluated at intermediate term follow-up. The first protocol, addressing computed tomography (CT)-optimized brachytherapy for advanced cervical cancer (n = 17), showed a significant reduction of D2cc for the bladder and sigmoid (p < 0.001) while maintaining a very high dose in D90 high-risk clinical target volume (HR-CTV) in comparison with standard point-based planning. In addition, after a follow-up of 19 months no tumor relapse was observed. The second UCP protocol, testing the impact of magnetic resonance imaging (MRI) guidance (n = 15) in patients with cervical cancer, proved significantly smaller D2cc doses for the bladder, rectum, and sigmoid (p = 0.003, p = 0.015, and p = 0.012), and secured highly curative mean doses in D90 HR-CTV of 99.2 Gy. The acute and late toxicity was excellent without any observed grade 3 or higher morbidity. In the third protocol, the combination of image-guided brachytherapy (IGBT) and whole pelvis intensity-modulated external beam radiotherapy (WP-IMRT) (n = 15) reaffirmed the significant reduction of D2cc doses for the bladder, rectum, and sigmoid (p = 0.001 or p < 0.001) along with high equivalent dose at 2 Gy (EQD2) in the HR-CTV, and demonstrated very low acute therapy-related toxicity in absence of grade 3 morbidity. The implementation of transabdominal ultrasound (TAUS) was the focus of the fourth UCP project aiming a more generous potential use of image-guidance on long-term, and enhancing the quality of soft tissue assessment complementary to conventionally planned

  2. Distribution and length of osteophytes in the lumbar vertebrae and risk of rupture of abdominal aortic aneurysms: a study of dry bones from Chiang Mai, Thailand.

    PubMed

    Chanapa, Patcharin; Yoshiyuki, Tohno; Mahakkanukrauh, Pasuk

    2014-09-01

    Vertebral osteophytes are a characteristic feature of intervertebral disc degeneration. In the lumbar spinal region, the two major structures in close proximity anterior to the spine are the inferior vena cava and the abdominal aorta, both of which have been reported to be affected by osteophytes. The purpose of this study was to determine the distribution, classification and lengths of osteophytes in the lumbar vertebrae. One hundred and eighty lumbar columns of 90 males and 90 females from Chiang Mai, Thailand, in the age range 15 to 96 years (mean age, 63 years) were collected. The measuring length of osteophytes was assessed on vertebral body and articular facet. Statistical analysis was performed by descriptive analysis, chi-square and Pearson Correlation. Lumbar osteophytes were presented in 175 specimens (97.2%), 88 males and 87 females. The highest frequency was at L4, most were on the superior, inferior surface of body and articular facet (39.7%, 38.4%, and 22%), respectively. The greatest mean length was 3.47±2.21 mm at L5, and the longest length of anterior superior surface of body was 28.56 mm. The osteophyte length was significantly correlated directly with age (P<0.01), and males were significantly greater than females (P<0.05). The highest prevalence of osteophytes was on the anterior side of superior surface of body (30.4%), and the classification was traction. It can be proposed that the abdominal aorta could be damaged, especially a risk of rupture of abdominal aortic aneurysm.

  3. Essential oils as potential adulticides against two populations of Aedes aegypti, the laboratory and natural field strains, in Chiang Mai province, northern Thailand.

    PubMed

    Chaiyasit, Dana; Choochote, Wej; Rattanachanpichai, Eumporn; Chaithong, Udom; Chaiwong, Prasong; Jitpakdi, Atchariya; Tippawangkosol, Pongsri; Riyong, Doungrat; Pitasawat, Benjawan

    2006-11-01

    Essential oils derived from five plant species, celery (Apium graveolens), caraway (Carum carvi), zedoary (Curcuma zedoaria), long pepper (Piper longum), and Chinese star anise (Illicium verum), were subjected to investigation of adulticidal activity against mosquito vectors. Two populations of Aedes aegypti, the laboratory and natural field strains, collected in Chiang Mai province, northern Thailand were tested in pyrethroid-susceptibility bioassays. The results revealed that the natural field strain of A. aegypti was resistant to permethrin, with mortality rates ranging from 51 to 66%. A mild susceptibility, with mortality rates ranging from 82 to 88%, was observed in the natural field strain of A. aegypti exposed to lambdacyhalothrin, which suggested that this strain was tolerant and might be resistant to this insecticide. However, laboratory-reared A. aegypti exposed to discriminating dosages of permethrin and lambdacyhalothrin induced 100% mortality in all cases, thus indicating complete susceptibility of this strain to these insecticides. The adulticidal activity determined by topical application revealed that all five essential oils exerted a promising adulticidal efficacy against both laboratory and natural field strains of A. aegypti. Although the laboratory strain was slightly more susceptible to these essential oils than the natural field strain, no statistically significant difference was observed. Moreover, comparison of the adulticidal activity indicated that the performance of these essential oils against the two strains of A. aegypti was similar. The highest potential was established from caraway, followed by zedoary, celery, long pepper, and Chinese star anise, with an LC(50) in the laboratory strain of 5.44, 5.94, 5.96, 6.21, and 8.52 microg/mg female, respectively, and 5.54, 6.02, 6.14, 6.35, and 8.83 microg/mg female, respectively, in the field strain. These promising essential oils are, therefore, an alternative in developing and producing

  4. Preliminary Study of Ground Movement in Prone Landslide Area by Means of MAI InSAR A Case Study: Ciloto, West Java, Indonesia

    NASA Astrophysics Data System (ADS)

    Hayati, Noorlaila; Riedel, Björn; Niemeier, Wolfgang

    2016-04-01

    Ciloto is one of the most prone landslide hazard areas in Indonesia. Several landslides in 2012 and 2013 had been recorded in Ciloto and damaged infrastructure around the area. Investigating the history of ground movement along slope area before the landslide happened could support the hazard mitigation in the future. Considering to an efficient surveying method, space-borne SAR processing is the one appropriate way to monitor the phenomenon in past years. The purpose of this study is detecting ground movement using multi-temporal synthetic aperture radar images. We use 13 ALOS PALSAR images from 2007 to 2009 with combination Fine Beam Single (FBS) and Fine Beam Double (FBD) polarization to investigate the slow movement on slope topography. MAI (Multiple Aperture Interferometry) InSAR method is used to analyze the ground movement from both line-of-sight and along-track direction. We split the synthetic aperture into two-looking aperture so that along-track displacement could be created by the difference of forward-backward looking interferograms. With integration of both methods, we could more precisely detect the movement in prone landslide area and achieve two measurements produced by the same interferogram. However, InSAR requires smaller baseline and good temporal baseline between master and slave images to avoid decorellation. There are only several pairs that meet the condition of proper length and temporal baseline indeed the location is also on the agriculture area where is mostly covered by vegetation. The result for two years observation shows that there is insignificant slow movement along slope surface in Ciloto with -2 - -7 cm in range looks or line of sight and 9-40 cm in along track direction. Based on geometry SAR , the most visible detecting of displacement is on the north-west area due to utilization of ascending SAR images.

  5. Dental fluorosis in populations from Chiang Mai, Thailand with different fluoride exposures – Paper 1: assessing fluorosis risk, predictors of fluorosis and the potential role of food preparation

    PubMed Central

    2012-01-01

    Background To determine the severity of dental fluorosis in selected populations in Chiang Mai, Thailand with different exposures to fluoride and to explore possible risk indicators for dental fluorosis. Methods Subjects were male and female lifetime residents aged 8–13 years. For each child the fluoride content of drinking and cooking water samples were assessed. Digital images were taken of the maxillary central incisors for later blind scoring for TF index (10% repeat scores). Interview data explored previous cooking and drinking water use, exposure to fluoride, infant feeding patterns and oral hygiene practices. Results Data from 560 subjects were available for analysis (298 M, 262 F). A weighted kappa of 0.80 was obtained for repeat photographic scores. The prevalence of fluorosis (TF 3+) for subjects consuming drinking and cooking water with a fluoride concentration of <0.9 ppm was 10.2%. For subjects consuming drinking and cooking water >0.9 ppm F the prevalence of fluorosis (TF 3+) rose to 37.3%. Drinking and cooking water at age 3, water used for infant formula and water used for preparing infant food all demonstrated an increase in fluorosis severity with increase in water fluoride level (p < 0.001). The probability estimate for the presentation of aesthetically significant fluorosis was 0.53 for exposure to high fluoride drinking (≥0.9 ppm) and cooking water (≥1.6 ppm). Conclusions The consumption of drinking water with fluoride content >0.9 ppm and use of cooking water with fluoride content >1.6 ppm were associated with an increased risk of aesthetically significant dental fluorosis. Fluoride levels in the current drinking and cooking water sources were strongly correlated with fluorosis severity. Further work is needed to explore fluorosis risk in relation to total fluoride intake from all sources including food preparation. PMID:22720834

  6. Child-education program for the reduction of health risks due to fluoride in water sources in the Chiang Mai Basin, Thailand.

    PubMed

    Takizawa, S; Takeda, T; Wongrueng, A; Wattanachira, S

    2010-01-01

    Groundwater is the major source of drinking water in Lamphun Province in the Chiang Mai Basin, Thailand. However, groundwater contains high fluoride up to 16 mg F/L, which has caused dental and skeletal fluorosis. Although Thai Government installed RO membrane plants for the removal of fluoride from groundwater; and delivers RO-filtered bottled water that contains less fluoride than the Thai Standard of 0.7 mg F/L, it was found that the urinary fluoride levels are still high among the residents. To find the major sources of fluoride intake, fluoride contents in various water sources, such as village water supply, shallow and deep groundwaters, rain water and bottled water, were measured, and the local people's behavior on water uses was recorded by interview and questionnaire study. As a result, it was found that the highest risk of fluoride ingestion comes from cooking rice with fluoride-containing water because of a lack of knowledge on fluoride sources and fluoride chemistry. To reduce the health risks arising from fluoride intake, a hands-on educational program on the sources and risks of fluoride in water was developed and implemented in the local schools. The participatory educational program promoted active involvement of schoolchildren, but it was found that the effectiveness of education varied depending on the questions we asked. Therefore, it needs to be improved by an iterative and interactive educational program. In conclusion, it was found that the benefits of providing safe drinking water using such advanced technology as RO membrane can be maximized only when it comes along with a participatory educational program on fluoride sources and health risks.

  7. Salmonella in pork retail outlets and dissemination of its pulsotypes through pig production chain in Chiang Mai and surrounding areas, Thailand.

    PubMed

    Patchanee, Prapas; Tansiricharoenkul, Kankanok; Buawiratlert, Tunyamai; Wiratsudakul, Anuwat; Angchokchatchawal, Kittipat; Yamsakul, Panuwat; Yano, Terdsak; Boonkhot, Phacharaporn; Rojanasatien, Suvichai; Tadee, Pakpoom

    2016-08-01

    Salmonella spp. is acknowledged as a significant zoonotic foodborne pathogen throughout the world. Contaminated pork consumption is considered as a main cause of human salmonellosis. In the later stage of the pig production chain, poor hygiene and unsuitable storage conditions in retail outlets are considered to be key factors linked to the risk of Salmonella infection. The purpose of current study, which was conducted throughout April 2014 to September 2014, was to determine the prevalence and characteristics of Salmonella spp. in pork sold at the retail stage in wet markets and supermarkets in the Chiang Mai urban area of Thailand. Additionally, clonal relations between Salmonella strains described in this study and those identified in earlier study from the same geographical area were considered. It is provided as a means of contributing to current knowledge regarding Salmonella epidemiology with an ultimate aim of improved food security and consumer protection in this region. From a total of 82 pork samples analyzed in this study, 41% were positive for Salmonella, with prevalence of 73.2% from wet markets (n=30/41) and 9.8% from supermarkets (n=4/41). Twelve Salmonella serovars were identified, S. Rissen being the most commonly encountered. Antibiotic resistance of the isolates was highest for ampicillin and tetracycline (53%), followed by streptomycin (44%). Pulsed-field gel electrophoresis (PFGE) and subsequent geographical distribution analysis indicated that the clonal Salmonella strains originated from multiple sources had been spread over a wide area. The existence of a common pig supply chain "farm-slaughterhouse-retail" transmission route is inferred. Continuous monitoring of Salmonella along the entire production chain is needed to reduce contamination loads and to ensure the safety of pork products for end consumers.

  8. Biosynthesis of silver and zinc oxide nanoparticles using Pichia fermentans JA2 and their antimicrobial property

    NASA Astrophysics Data System (ADS)

    Chauhan, Ritika; Reddy, Arpita; Abraham, Jayanthi

    2015-01-01

    The development of eco-friendly alternative to chemical synthesis of metal nanoparticles is of great challenge among researchers. The present study aimed to investigate the biological synthesis, characterization, antimicrobial study and synergistic effect of silver and zinc oxide nanoparticles against clinical pathogens using Pichia fermentans JA2. The extracellular biosynthesis of silver and zinc oxide nanoparticles was investigated using Pichia fermentans JA2 isolated from spoiled fruit pulp bought in Vellore local market. The crystalline and stable metallic nanoparticles were characterized evolving several analytical techniques including UV-visible spectrophotometer, X-ray diffraction pattern analysis and FE-scanning electron microscope with EDX-analysis. The biosynthesized metallic nanoparticles were tested for their antimicrobial property against medically important Gram positive, Gram negative and fungal pathogenic microorganisms. Furthermore, the biosynthesized nanoparticles were also evaluated for their increased antimicrobial activities with various commercially available antibiotics against clinical pathogens. The biosynthesized silver nanoparticles inhibited most of the Gram negative clinical pathogens, whereas zinc oxide nanoparticles were able to inhibit only Pseudomonas aeruginosa. The combined effect of standard antibiotic disc and biosynthesized metallic nanoparticles enhanced the inhibitory effect against clinical pathogens. The biological synthesis of silver and zinc oxide nanoparticles is a novel and cost-effective approach over harmful chemical synthesis techniques. The metallic nanoparticles synthesized using Pichia fermentans JA2 possess potent inhibitory effect that offers valuable contribution to pharmaceutical associations.

  9. The prognostic value of mitotic activity index (MAI), phosphohistone H3 (PPH3), cyclin B1, cyclin A, and Ki67, alone and in combinations, in node-negative premenopausal breast cancer.

    PubMed

    Klintman, Marie; Strand, Carina; Ahlin, Cecilia; Beglerbegovic, Sanda; Fjällskog, Marie-Louise; Grabau, Dorthe; Gudlaugsson, Einar; Janssen, Emiel A M; Lövgren, Kristina; Skaland, Ivar; Bendahl, Pär-Ola; Malmström, Per; Baak, Jan P A; Fernö, Mårten

    2013-01-01

    Proliferation, either as the main common denominator in genetic profiles, or in the form of single factors such as Ki67, is recommended for clinical use especially in estrogen receptor-positive (ER) patients. However, due to high costs of genetic profiles and lack of reproducibility for Ki67, studies on other proliferation factors are warranted. The aim of the present study was to evaluate the prognostic value of the proliferation factors mitotic activity index (MAI), phosphohistone H3 (PPH3), cyclin B1, cyclin A and Ki67, alone and in combinations. In 222 consecutive premenopausal node-negative breast cancer patients (87% without adjuvant medical treatment), MAI was assessed on whole tissue sections (predefined cut-off ≥10 mitoses), and PPH3, cyclin B1, cyclin A, and Ki67 on tissue microarray (predefined cut-offs 7th decile). In univariable analysis (high versus low) the strongest prognostic proliferation factor for 10-year distant disease-free survival was MAI (Hazard Ratio (HR)=3.3, 95% Confidence Interval (CI): 1.8-6.1), followed by PPH3, cyclin A, Ki67, and cyclin B1. A combination variable, with patients with MAI and/or cyclin A high defined as high-risk, had even stronger prognostic value (HR=4.2, 95%CI: 2.2-7). When stratifying for ER-status, MAI was a significant prognostic factor in ER-positive patients only (HR=7.0, 95%CI: 3.1-16). Stratified for histological grade, MAI added prognostic value in grade 2 (HR=7.2, 95%CI: 3.1-38) and grade 1 patients. In multivariable analysis including HER2, age, adjuvant medical treatment, ER, and one proliferation factor at a time, only MAI (HR=2.7, 95%CI: 1.1-6.7), and cyclin A (HR=2.7, 95%CI: 1.2-6.0) remained independently prognostic. In conclusion this study confirms the strong prognostic value of all proliferation factors, especially MAI and cyclin A, in all patients, and more specifically in ER-positive patients, and patients with histological grade 2 and 1. Additionally, by combining two proliferation factors

  10. The crosstalk between Target of Rapamycin (TOR) and Jasmonic Acid (JA) signaling existing in Arabidopsis and cotton

    PubMed Central

    Song, Yun; Zhao, Ge; Zhang, Xueyan; Li, Linxuan; Xiong, Fangjie; Zhuo, Fengping; Zhang, Chaojun; Yang, Zuoren; Datla, Raju; Ren, Maozhi; Li, Fuguang

    2017-01-01

    Target of rapamycin (TOR) acts as an important regulator of cell growth, development and stress responses in most examined diploid eukaryotes. However, little is known about TOR in tetraploid species such as cotton. Here, we show that TORC1-S6K-RPS6, the major signaling components, are conserved and further expanded in cotton genome. Though the cotton seedlings are insensitive to rapamycin, AZD8055, the second-generation inhibitor of TOR, can significantly suppress the growth in cotton. Global transcriptome analysis revealed that genes associated with jasmonic acid (JA) biosynthesis and transduction were significantly altered in AZD8055 treated cotton seedlings, suggesting the potential crosstalk between TOR and JA signaling. Pharmacological and genetic approaches have been employed to get further insights into the molecular mechanism of the crosstalk between TOR and JA. Combination of AZD8055 with methyl jasmonate can synergistically inhibit cotton growth, and additionally JA levels were significantly increased when cotton seedlings were subjected to AZD8055. JA biosynthetic and signaling mutants including jar1, coi1-2 and myc2-2 displayed TOR inhibitor-resistant phenotypes, whereas COI1 overexpression transgenic lines and jaz10 exhibited sensitivity to AZD8055. Consistently, cotton JAZ can partially rescue TOR-suppressed phenotypes in Arabidopsis. These evidences revealed that the crosstalk between TOR and JA pathway operates in cotton and Arabidopsis. PMID:28374843

  11. Flavone-resistant Leishmania donovani overexpresses LdMRP2 transporter in the parasite and activates host MRP2 on macrophages to circumvent the flavone-mediated cell death.

    PubMed

    Chowdhury, Sayan; Mukhopadhyay, Rupkatha; Saha, Sourav; Mishra, Amartya; Sengupta, Souvik; Roy, Syamal; Majumder, Hemanta K

    2014-06-06

    In parasites, ATP-binding cassette (ABC) transporters represent an important family of proteins related to drug resistance and other biological activities. Resistance of leishmanial parasites to therapeutic drugs continues to escalate in developing countries, and in many instances, it is due to overexpressed ABC efflux pumps. Progressively adapted baicalein (BLN)-resistant parasites (pB(25)R) show overexpression of a novel ABC transporter, which was classified as ABCC2 or Leishmania donovani multidrug resistance protein 2 (LdMRP2). The protein is primarily localized in the flagellar pocket region and in internal vesicles. Overexpressed LdABCC2 confers substantial BLN resistance to the parasites by rapid drug efflux. The BLN-resistant promastigotes when transformed into amastigotes in macrophage cells cannot be cured by treatment of macrophages with BLN. Amastigote resistance is concomitant with the overexpression of macrophage MRP2 transporter. Reporter analysis and site-directed mutagenesis assays demonstrated that antioxidant response element 1 is activated upon infection. The expression of this phase II detoxifying gene is regulated by NFE2-related factor 2 (Nrf2)-mediated antioxidant response element activation. In view of the fact that the signaling pathway of phosphoinositol 3-kinase controls microfilament rearrangement and translocation of actin-associated proteins, the current study correlates with the intricate pathway of phosphoinositol 3-kinase-mediated nuclear translocation of Nrf2, which activates MRP2 expression in macrophages upon infection by the parasites. In contrast, phalloidin, an agent that prevents depolymerization of actin filaments, inhibits Nrf2 translocation and Mrp2 gene activation by pB(25)R infection. Taken together, these results provide insight into the mechanisms by which resistant clinical isolates of L. donovani induce intracellular events relevant to drug resistance.

  12. Flavone-resistant Leishmania donovani Overexpresses LdMRP2 Transporter in the Parasite and Activates Host MRP2 on Macrophages to Circumvent the Flavone-mediated Cell Death*

    PubMed Central

    Chowdhury, Sayan; Mukhopadhyay, Rupkatha; Saha, Sourav; Mishra, Amartya; Sengupta, Souvik; Roy, Syamal; Majumder, Hemanta K.

    2014-01-01

    In parasites, ATP-binding cassette (ABC) transporters represent an important family of proteins related to drug resistance and other biological activities. Resistance of leishmanial parasites to therapeutic drugs continues to escalate in developing countries, and in many instances, it is due to overexpressed ABC efflux pumps. Progressively adapted baicalein (BLN)-resistant parasites (pB25R) show overexpression of a novel ABC transporter, which was classified as ABCC2 or Leishmania donovani multidrug resistance protein 2 (LdMRP2). The protein is primarily localized in the flagellar pocket region and in internal vesicles. Overexpressed LdABCC2 confers substantial BLN resistance to the parasites by rapid drug efflux. The BLN-resistant promastigotes when transformed into amastigotes in macrophage cells cannot be cured by treatment of macrophages with BLN. Amastigote resistance is concomitant with the overexpression of macrophage MRP2 transporter. Reporter analysis and site-directed mutagenesis assays demonstrated that antioxidant response element 1 is activated upon infection. The expression of this phase II detoxifying gene is regulated by NFE2-related factor 2 (Nrf2)-mediated antioxidant response element activation. In view of the fact that the signaling pathway of phosphoinositol 3-kinase controls microfilament rearrangement and translocation of actin-associated proteins, the current study correlates with the intricate pathway of phosphoinositol 3-kinase-mediated nuclear translocation of Nrf2, which activates MRP2 expression in macrophages upon infection by the parasites. In contrast, phalloidin, an agent that prevents depolymerization of actin filaments, inhibits Nrf2 translocation and Mrp2 gene activation by pB25R infection. Taken together, these results provide insight into the mechanisms by which resistant clinical isolates of L. donovani induce intracellular events relevant to drug resistance. PMID:24706751

  13. RoXaN, a Novel Cellular Protein Containing TPR, LD, and Zinc Finger Motifs, Forms a Ternary Complex with Eukaryotic Initiation Factor 4G and Rotavirus NSP3

    PubMed Central

    Vitour, Damien; Lindenbaum, Pierre; Vende, Patrice; Becker, Michelle M.; Poncet, Didier

    2004-01-01

    Rotavirus mRNAs are capped but not polyadenylated, and viral proteins are translated by the cellular translation machinery. This is accomplished through the action of the viral nonstructural protein NSP3, which specifically binds the 3′ consensus sequence of viral mRNAs and interacts with the eukaryotic translation initiation factor eIF4G I. To further our understanding of the role of NSP3 in rotavirus replication, we looked for other cellular proteins capable of interacting with this viral protein. Using the yeast two-hybrid assay, we identified a novel cellular protein-binding partner for rotavirus NSP3. This 110-kDa cellular protein, named RoXaN (rotavirus X protein associated with NSP3), contains a minimum of three regions predicted to be involved in protein-protein or nucleic acid-protein interactions. A tetratricopeptide repeat region, a protein-protein interaction domain most often found in multiprotein complexes, is present in the amino-terminal region. In the carboxy terminus, at least five zinc finger motifs are observed, further suggesting the capacity of RoXaN to bind other proteins or nucleic acids. Between these two regions exists a paxillin leucine-aspartate repeat (LD) motif which is involved in protein-protein interactions. RoXaN is capable of interacting with NSP3 in vivo and during rotavirus infection. Domains of interaction were mapped and correspond to the dimerization domain of NSP3 (amino acids 163 to 237) and the LD domain of RoXaN (amino acids 244 to 341). The interaction between NSP3 and RoXaN does not impair the interaction between NSP3 and eIF4G I, and a ternary complex made of NSP3, RoXaN, and eIF4G I can be detected in rotavirus-infected cells, implicating RoXaN in translation regulation. PMID:15047801

  14. Chiang Mai University Health Worker Study aiming toward a better understanding of noncommunicable disease development in Thailand: methods and description of study population

    PubMed Central

    Angkurawaranon, Chaisiri; Wisetborisut, Anawat; Jiraporncharoen, Wichuda; Likhitsathian, Surinporn; Uaphanthasath, Ronnaphob; Gomutbutra, Patama; Jiraniramai, Surin; Lerssrimonkol, Chawin; Aramrattanna, Apinun; Doyle, Pat; Nitsch, Dorothea

    2014-01-01

    Background Urbanization is considered to be one of the key drivers of noncommunicable diseases (NCDs) in Thailand and other developing countries. These influences, in turn, may affect an individual’s behavior and risk of developing NCDs. The Chiang Mai University (CMU) Health Worker Study aims to provide evidence for a better understanding of the development of NCDs and ultimately to apply the evidence toward better prevention, risk modification, and improvement of clinical care for patients with NCDs and NCD-related conditions. Methods A cross-sectional survey of health care workers from CMU Hospital was conducted between January 2013 and June 2013. Questionnaires, interviews, and physical and laboratory examinations were used to assess urban exposure, occupational shift work, risk factors for NCDs, self-reported NCDs, and other NCD-related health conditions. Results From 5,364 eligible workers, 3,204 participated (59.7%). About 11.1% of the participants had high blood pressure (systolic blood pressure ≥140 mmHg or diastolic blood pressure ≥90 mmHg) and almost 30% were considered to be obese (body mass index ≥25 kg/m2). A total of 2.3% had a high fasting blood glucose level (≥126 mg/dL), and the most common abnormal lipid profile was high low-density lipoprotein (≥160 mg/dL), which was found in 19.2% of participants. Discussion The study of health workers offers three potential advantages. The first is that the study of migrants was possible. Socioenvironmental influence on NCD risk factors can be explored, as changes in environmental exposures can be documented. Second, it allows the investigators to control for access to care. Access to care is potentially a key confounder toward understanding the development of NCDs. Lastly, a study of health personnel allows easy access to laboratory investigations and potential for long-term follow-up. This enables ascertainment of a number of clinical outcomes and provides potential for future studies focusing on

  15. Priming for JA-dependent defenses using hexanoic acid is an effective mechanism to protect Arabidopsis against B. cinerea.

    PubMed

    Kravchuk, Zhana; Vicedo, Begonya; Flors, Víctor; Camañes, Gemma; González-Bosch, Carmen; García-Agustín, Pilar

    2011-03-01

    Soil drench treatments with hexanoic acid can effectively protect Arabidopsis plants against Botrytis cinerea through a mechanism based on a stronger and faster accumulation of JA-dependent defenses. Plants impaired in ethylene, salicylic acid, abscisic acid or glutathion pathways showed intact protection by hexanoic acid upon B. cinerea infection. Accordingly, no significant changes in the SA marker gene PR-1 in either the SA or ABA hormone balance were observed in the infected and treated plants. In contrast, the JA signaling pathway showed dramatic changes after hexanoic acid treatment, mainly when the pathogen was present. The impaired JA mutants, jin1-2 and jar1, were unable to display hexanoic acid priming against the necrotroph. In addition, hexanoic acid-treated plants infected with B. cinerea showed priming in the expression of the PDF1.2, PR-4 and VSP1 genes implicated in the JA pathways. Moreover, JA and OPDA levels were primed at early stages by hexanoic acid. Treatments also stimulated increased callose accumulation in response to the pathogen. Although callose accumulation has proved an effective IR mechanism against B. cinerea, it is apparently not essential to express hexanoic acid-induced resistance (HxAc-IR) because the mutant pmr4.1 (callose synthesis defective mutant) is protected by treatment. We recently described how hexanoic acid treatments can protect tomato plants against B. cinerea by stimulating ABA-dependent callose deposition and by priming OPDA and JA-Ile production. We clearly demonstrate here that Hx-IR is a dependent plant species, since this acid protects Arabidopsis plants against the same necrotroph by priming JA-dependent defenses without enhancing callose accumulation.

  16. Rice Rab11 is required for JA-mediated defense signaling

    SciTech Connect

    Hong, Min Ji; Lee, Yun mi; Son, Young Sim; Im, Chak Han; Yi, Young Byung; Rim, Yeong Gil; Bahk, Jeong Dong; Heo, Jae Bok

    2013-05-17

    Highlights: •OsRab11 interacts with OsOPR8. •OsOPR8 is localized in the cytosol and peroxisome. •OsRab11 enhances the NADPH consumption by OsOPR8. •Transgenic Arabidopsis overexpressing OsRab11 represents a pathogen-resistant phenotype. -- Abstract: Rab proteins play an essential role in regulating vesicular transport in eukaryotic cells. Previously, we characterized OsRab11, which in concert with OsGAP1 and OsGDI3 regulates vesicular trafficking from the trans-Golgi network (TGN) to the plasma membrane or vacuole. To further elucidate the physiological function of OsRab11 in plants, we performed yeast two-hybrid screens using OsRab11 as bait. OsOPR8 was isolated and shown to interact with OsRab11. A co-immunoprecipitation assay confirmed this interaction. The green fluorescent protein-OsOPR8 fusion product was targeted to the cytoplasm and peroxisomes of protoplasts from Arabidopsis thaliana. OsOPR8 exhibited NADPH-dependent reduction activity when 2-cyclohexen-1-one (CyHE) and 12-oxo-phytodienoic acid (OPDA) were supplied as possible substrates. Interestingly, NADPH oxidation by OsOPR8 was increased when wild-type OsRab11 or the constitutively active form of OsRab11 (Q78L) were included in the reaction mix, but not when the dominant negative form of OsRab11 (S28N) was included. OsRab11 was expressed broadly in plants and both OsRab11 and OsOPR8 were induced by jasmonic acid (JA) and elicitor treatments. Overexpressed OsRab11 transgenic plants showed resistance to pathogens through induced expression of JA-responsive genes. In conclusion, OsRab11 may be required for JA-mediated defense signaling by activating the reducing activity of OsOPR8.

  17. PM10-bound polycyclic aromatic hydrocarbons in Chiang Mai (Thailand): Seasonal variations, source identification, health risk assessment and their relationship to air-mass movement

    NASA Astrophysics Data System (ADS)

    Wiriya, Wan; Prapamontol, Tippawan; Chantara, Somporn

    2013-04-01

    This study aims to analyze the seasonal variations of PM10-bound polycyclic aromatic hydrocarbons (PAHs) for an estimation of the human health risk and identification of their possible sources. Ninety four PM10 samples were collected during the dry and wet seasons of 2010 and the dry season of 2011 in Chiang Mai, Thailand, and analyzed for 16 PAHs by gas chromatography-mass spectrometry. The average PM10 concentrations were 104.91 ± 32.70, 13.28 ± 11.34 and 36.24 ± 19.16 μg/m3 in dry season of 2010, wet season of 2010 and dry season of 2011, respectively, while the average 16-PAHs concentrations were 25.87 ± 10.13, 3.12 ± 2.18 and 4.58 ± 2.18 ng/m3, respectively. Correlations of PM10 and total PAHs concentrations were relatively high during all seasons (r > 0.796). In addition, PM10 concentrations were highly correlated with carcinogenic PAHs (r = 0.927) during the dry season of 2010, indicating that carcinogenic compounds were dominant in the particulate PAHs and could be generated from open burning, usually conducted in the dry season. The average PM10 concentration in the dry season of 2011 was much lower than that in 2010 and lower than the annual average of the past 12 years (48.17 μg/m3) because of the unusually high amount of rain precipitation and low open burning activity in this year. According to the accumulated number of hot spots occurring in northern part of Thailand, approximately 19,000 spots were found in the dry season of 2010, while only 6,600 spots were found in the dry season of 2011. It can be seen that larger scale open burning activities were performed in the dry season of 2010 than in the dry season of 2011. The value of toxicity equivalent concentration from PAHs in the dry season of 2010 was higher than that of the wet season of 2010 and the dry season of 2011. This is obviously related to concentrations of PM10 and PAHs. Diagnostic ratio and principal component analysis were used to find out the sources of PM10-bound PAHs. It was

  18. MAPK-dependent JA and SA signalling in Nicotiana attenuata affects plant growth and fitness during competition with conspecifics

    PubMed Central

    2012-01-01

    Background Induced defense responses to herbivores are generally believed to have evolved as cost-saving strategies that defer the fitness costs of defense metabolism until these defenses are needed. The fitness costs of jasmonate (JA)-mediated defenses have been well documented. Those of the early signaling units mediating induced resistance to herbivores have yet to be examined. Early signaling components that mediate herbivore-induced defense responses in Nicotiana attenuata, have been well characterized and here we examine their growth and fitness costs during competition with conspecifics. Two mitogen-activated protein kinases (MAPKs), salicylic acid (SA)-induced protein kinase (SIPK) and wound-induced protein kinase (WIPK) are rapidly activated after perception of herbivory and both kinases regulate herbivory-induced JA levels and JA-mediated defense metabolite accumulations. Since JA-induced defenses result in resource-based trade-offs that compromise plant productivity, we evaluated if silencing SIPK (irSIPK) and WIPK (irWIPK) benefits the growth and fitness of plants competiting with wild type (WT) plants, as has been shown for plants silenced in JA-signaling by the reduction of Lipoxygenase 3 (LOX3) levels. Results As expected, irWIPK and LOX3-silenced plants out-performed their competing WT plants. Surprisingly, irSIPK plants, which have the largest reductions in JA signaling, did not. Phytohormone profiling of leaves revealed that irSIPK plants accumulated higher levels of SA compared to WT. To test the hypothesis that these high levels of SA, and their presumed associated fitness costs of pathogen associated defenses in irSIPK plants had nullified the JA-deficiency-mediated growth benefits in these plants, we genetically reduced SA levels in irSIPK plants. Reducing SA levels partially recovered the biomass and fitness deficits of irSIPK plants. We also evaluated whether the increased fitness of plants with reduced SA or JA levels resulted from

  19. The tomato res mutant which accumulates JA in roots in non-stressed conditions restores cell structure alterations under salinity.

    PubMed

    Garcia-Abellan, José O; Fernandez-Garcia, Nieves; Lopez-Berenguer, Carmen; Egea, Isabel; Flores, Francisco B; Angosto, Trinidad; Capel, Juan; Lozano, Rafael; Pineda, Benito; Moreno, Vicente; Olmos, Enrique; Bolarin, Maria C

    2015-11-01

    Jasmonic acid (JA) regulates a wide spectrum of plant biological processes, from plant development to stress defense responses. The role of JA in plant response to salt stress is scarcely known, and even less known is the specific response in root, the main plant organ responsible for ionic uptake and transport to the shoot. Here we report the characterization of the first tomato (Solanum lycopersicum) mutant, named res (restored cell structure by salinity), that accumulates JA in roots prior to exposure to stress. The res tomato mutant presented remarkable growth inhibition and displayed important morphological alterations and cellular disorganization in roots and leaves under control conditions, while these alterations disappeared when the res mutant plants were grown under salt stress. Reciprocal grafting between res and wild type (WT) (tomato cv. Moneymaker) indicated that the main organ responsible for the development of alterations was the root. The JA-signaling pathway is activated in res roots prior to stress, with transcripts levels being even higher in control condition than in salinity. Future studies on this mutant will provide significant advances in the knowledge of JA role in root in salt-stress tolerance response, as well as in the energy trade-off between plant growth and response to stress.

  20. OsJAR1 is required for JA-regulated floret opening and anther dehiscence in rice.

    PubMed

    Xiao, Yuguo; Chen, Yi; Charnikhova, Tatsiana; Mulder, Patrick P J; Heijmans, Jeroen; Hoogenboom, Angela; Agalou, Adamantia; Michel, Corinne; Morel, Jean-Benoit; Dreni, Ludovico; Kater, Martin M; Bouwmeester, Harro; Wang, Mei; Zhu, Zhen; Ouwerkerk, Pieter B F

    2014-09-01

    Jasmonates are important phytohormones regulating reproductive development. We used two recessive rice Tos17 alleles of OsJAR1, osjar1-2 and osjar1-3, to study the biological function of jasmonates in rice anthesis. The florets of both osjar1 alleles stayed open during anthesis because the lodicules, which control flower opening in rice, were not withering on time. Furthermore, dehiscence of the anthers filled with viable pollen, was impaired, resulting in lower fertility. In situ hybridization and promoter GUS transgenic analysis confirmed OsJAR1 expression in these floral tissues. Flower opening induced by exogenous applied methyl jasmonate was impaired in osjar1 plants and was restored in a complementation experiment with transgenics expressing a wild type copy of OsJAR1 controlled by a rice actin promoter. Biochemical analysis showed that OsJAR1 encoded an enzyme conjugating jasmonic acid (JA) to at least Ile, Leu, Met, Phe, Trp and Val and both osjar1 alleles had substantial reduction in content of JA-Ile, JA-Leu and JA-Val in florets. We conclude that OsJAR1 is a JA-amino acid synthetase that is required for optimal flower opening and closing and anther dehiscence in rice.

  1. Kinetics and regulation of lactose transport and metabolism in Kluyveromyces lactis JA6.

    PubMed

    Santos, A M; Silveira, W B; Fietto, L G; Brandão, R L; Castro, I M

    2014-07-01

    Kluyveromyces lactis strains are able to assimilate lactose. They have been used industrially to eliminate this sugar from cheese whey and in other industrial products. In this study, we investigated specific features and the kinetic parameters of the lactose transport system in K. lactis JA6. In lactose grown cells, lactose was transported by a system transport with a half-saturation constant (K s) of 1.49 ± 0.38 mM and a maximum velocity (V max) of 0.96 ± 0.12 mmol. (g dry weight)(-1) h(-1) for lactose. The transport system was constitutive and energy-dependent. Results obtained by different approaches showed that the lactose transport system was regulated by glucose at the transcriptional level and by glucose and other sugars at a post-translational level. In K. lactis JA6, galactose metabolization was under glucose control. These findings indicated that the regulation of lactose-galactose regulon in K. lactis was similar to the regulation of galactose regulon in Saccharomyces cerevisiae.

  2. Early dust formation and a massive progenitor for SN 2011ja?

    NASA Astrophysics Data System (ADS)

    Andrews, J. E.; Krafton, Kelsie M.; Clayton, Geoffrey C.; Montiel, E.; Wesson, R.; Sugerman, Ben E. K.; Barlow, M. J.; Matsuura, M.; Drass, H.

    2016-04-01

    SN 2011ja was a bright (I = -18.3) Type II supernova occurring in the nearby edge on spiral galaxy NGC 4945. Flat-topped and multipeaked H α and H β spectral emission lines appear between 64 and 84 d post-explosion, indicating interaction with a disc-like circumstellar medium inclined ˜45° from edge-on. After day 84, an increase in the H- and K-band flux along with heavy attenuation of the red wing of the emission lines are strong indications of early dust formation, likely located in the cool dense shell created between the forward shock of the SN ejecta and the reverse shock created as the ejecta plows into the existing circumstellar material. Radiative transfer modelling reveals both ≈1 × 10-5 M⊙ of pre-existing dust located ˜1016.7 cm away and up to ≈6 × 10-4 M⊙ of newly formed dust. Spectral observations after 1.5 yr reveal the possibility that the fading SN is located within a young (3-6 Myr) massive stellar cluster, which when combined with tentative 56Ni mass estimates of 0.2 M⊙ may indicate a massive (≥25 M⊙) progenitor for SN 2011ja.

  3. Draft genome sequence of Rhodomicrobium udaipurense JA643T with special reference to hopanoid biosynthesis.

    PubMed

    Tushar, L; Sasikala, Ch; Ramana, Ch V

    2014-12-01

    Hopanoids are present in vast amounts as integral components of bacteria and plants with their primary function to strengthen rigidity of the plasma membrane. To establish their roles more precisely, we conducted sequencing of the whole genome of Rhodomicrobium udaipurense JA643(T) isolated from a fresh water stream of Udaipur in Himachal Pradesh, India, by using the Illumina HiSeq pair end chemistry of 2 × 100 bp platform. Determined genome showed a high degree of similarity to the genome of R. vannielii ATCC17100(T) and the 13.7 million reads generated a sequence of 3,649,277 bp possessing 3,611 putative genes. The genomic data were subsequently investigated with respect to genes involved in various features. The machinery required for the degradation of aromatic compounds and resistance to solvents as well as all that required for photosynthesis are present in this organism. Also, through extensive functional annotation, 18 genes involved in the biosynthesis of hopanoids are predicted, namely those responsible for the synthesis of diploptene, diplopterol, adenosylhopane, ribosylhopane, aminobacteriohopanetriol, glycosyl group containing hopanoids and unsaturated hopanoids. The hopanoid biosynthetic pathway was then inferred based on the genes identified and through experimental validation of individual hopanoid molecules. The genome data of R. udaipurense JA643(T) will be useful in understanding the functional features of hopanoids in this bacterium.

  4. Acute oral toxicity of ja-2 solid propellant in sprague-dawley rats. Report for 12 November-19 December 1985

    SciTech Connect

    Brown, L.D.; Justus, J.D.; Wheeler, C.R.; Korte, D.W.

    1989-12-01

    The acute oral toxicity of JA-2 Solid Propellant was determined in male and female Sprague-Dawley rats by using an oral gavage split-dose method. The MLD was 3990.6 + or - 349.7 mg/kg for male rats and 2545.9 + or - 421.1 mg/kg for female rats. JA-2 produced clinical signs that were attributed to its nitrate ester components, diethyleneglycol dinitrate and nitroglycerin. These signs included tremors and twitching, cyanosis, and increases in respiratory rate and depth. Other clinical signs observed were associated with the general malaise of the animals following dosing and included hunched posture, rough coat, reddish stains around the eyes and nose, and perianal staining. Most animals exhibited signs by 4 hours after dosing and either had died or the signs had cleared by 96 hours after dosing. According to the classification scheme of Hodge and Sterner, these results place JA-2 in the slightly toxic class.

  5. Acute oral toxicity of JA-2 solid propellant in icr mice. Report for 17 December 1985-17 January 1986

    SciTech Connect

    Morgan, E.W.; Frost, D.F.; Wheller, C.R.; Korte, D.W.

    1989-12-01

    The acute oral toxicity of JA-2 Solid Propellant was determined in male and female ICR mice by using an oral gavage, split-dose method. The MLD was 3774.6 + or - 150.5 mg/kg for male mice and 3528.8 + or - 133.8 mg/kg for female mice. JA-2 produced component, diethyleneglycol dinitrate and nitroglycerin. These signs included tremors, inactivity, depression of reflexes, loss of equilibrium, opisthotonus, and increased respiratory activity. Other clinical signs observed were associated with the general malaise of the animals following dosing and included perianal staining, hunched posture, squinting, and rough coat. Most animals exhibited signs by 2 hours after dosing and either had died or the signs had cleared within 5 days of dosing. According to the classification scheme of Hodge and Sterner, these results place JA-2 in the slightly toxic class.

  6. Transcriptome sequencing and de novo analysis of cytoplasmic male sterility and maintenance in JA-CMS cotton.

    PubMed

    Yang, Peng; Han, Jinfeng; Huang, Jinling

    2014-01-01

    Cytoplasmic male sterility (CMS) is the failure to produce functional pollen, which is inherited maternally. And it is known that anther development is modulated through complicated interactions between nuclear and mitochondrial genes in sporophytic and gametophytic tissues. However, an unbiased transcriptome sequencing analysis of CMS in cotton is currently lacking in the literature. This study compared differentially expressed (DE) genes of floral buds at the sporogenous cells stage (SS) and microsporocyte stage (MS) (the two most important stages for pollen abortion in JA-CMS) between JA-CMS and its fertile maintainer line JB cotton plants, using the Illumina HiSeq 2000 sequencing platform. A total of 709 (1.8%) DE genes including 293 up-regulated and 416 down-regulated genes were identified in JA-CMS line comparing with its maintainer line at the SS stage, and 644 (1.6%) DE genes with 263 up-regulated and 381 down-regulated genes were detected at the MS stage. By comparing the two stages in the same material, there were 8 up-regulated and 9 down-regulated DE genes in JA-CMS line and 29 up-regulated and 9 down-regulated DE genes in JB maintainer line at the MS stage. Quantitative RT-PCR was used to validate 7 randomly selected DE genes. Bioinformatics analysis revealed that genes involved in reduction-oxidation reactions and alpha-linolenic acid metabolism were down-regulated, while genes pertaining to photosynthesis and flavonoid biosynthesis were up-regulated in JA-CMS floral buds compared with their JB counterparts at the SS and/or MS stages. All these four biological processes play important roles in reactive oxygen species (ROS) homeostasis, which may be an important factor contributing to the sterile trait of JA-CMS. Further experiments are warranted to elucidate molecular mechanisms of these genes that lead to CMS.

  7. Comparative study of the assay of Artemia salina L. and the estimate of the medium lethal dose (LD50 value) in mice, to determine oral acute toxicity of plant extracts.

    PubMed

    Logarto Parra, A; Silva Yhebra, R; Guerra Sardiñas, I; Iglesias Buela, L

    2001-09-01

    Artemia salina L. (Artemiidae), the brine shrimp larva, is an invertebrate used in the alternative test to determine toxicity of chemical and natural products. In this study the Medium Lethal Concentrations (LC50 value) of 20 plant extracts, Aloe vera (L.) Burm. F. (Aloeaceae), Artemisia absinthium L. (Asteraceae); Citrus aurantium L. (Rutaceae); Cymbopogon citratus (DC. Ex Nees) Stapf (Poaceae); Datura stramonium L. (Solanaceae); Justicia pectoralis Jacq. (Acanthaceae); Musa x paradisiaca L. (Musaceae); Ocimum basilicum L.; O. gratissimum L.; O. tenuiflorum L. (Lamiaceae); Pimenta dioica (L.) Merr. (Myrtaceae); Piper auritum Kunth (Piperaceae); Plantago major L. (Plantaginaceae); Plectranthus amboinicus (Lour.) Spreng. (Lamiaceae); Ruta graveolens L. (Rutaceae); Senna alata (L.) Roxb. (Fabaceae); Stachytarpheta jamaicensis (L.) Vahl (Verbenaceae); and Thuja occidentalis L. (Cupressaceae), were determined using Artemia salina L. (Artemiidae), with the objective of relating the results to the LD50 values reported in mice (tested at three concentrations: 10, 100, and 1000 microg/mL, for each extract). We found good correlation between the in vivo and the in vitro tests (r = 0.85 p < 0.05), and this method is a useful tool for predicting oral acute toxicity in plant extracts.

  8. Assessment of the predictive capacity of the 3T3 Neutral Red Uptake cytotoxicity test method to identify substances not classified for acute oral toxicity (LD50>2000 mg/kg): results of an ECVAM validation study.

    PubMed

    Prieto, Pilar; Cole, Thomas; Curren, Rodger; Gibson, Rosemary M; Liebsch, Manfred; Raabe, Hans; Tuomainen, Anita M; Whelan, Maurice; Kinsner-Ovaskainen, Agnieszka

    2013-04-01

    Assessing chemicals for acute oral toxicity is a standard information requirement of regulatory testing. However, animal testing is now prohibited in the cosmetics sector in Europe, and strongly discouraged for industrial chemicals. Building on the results of a previous international validation study, a follow up study was organised to assess if the 3T3 Neutral Red Uptake cytotoxicity assay could identify substances not requiring classification as acute oral toxicants under the EU regulations. Fifty-six coded industrial chemicals were tested in three laboratories, each using one of the following protocols: the previously validated protocol, an abbreviated version of the protocol and the protocol adapted for an automation platform. Predictions were very similar among the three laboratories. The assay exhibited high sensitivity (92-96%) but relatively low specificity (40-44%). Three chemicals were under predicted. Assuming that most industrial chemicals are not likely to be acutely toxic, this test method could prove a valuable component of an integrated testing strategy, a read-across argument, or weight-of-evidence approach to identify non toxic chemicals (LD50>2000 mg/kg). However, it is likely to under predict chemicals acting via specific mechanisms of action not captured by the 3T3 test system, or which first require biotransformation in vivo.

  9. Positive Suggestion: It Helps LD Students Learn.

    ERIC Educational Resources Information Center

    Oldridge, O. A. "Buff"

    1982-01-01

    The author reviews his experiences in remediating reading problems of learning disabled students through hypnotic and nonhypnotic suggestion. Research on the use of hypnosis is briefly summarized and recommendations on the use of nonhypnotic suggestion in the classroom are given. (CL)

  10. Organizing Communication for the LD Teacher.

    ERIC Educational Resources Information Center

    Safran, Joan; Safran, Stephen P.

    1985-01-01

    A model depicting teacher-to-teacher communication can serve as a guide for special class teachers to systematize and streamline communication with regular teachers of mainstreamed learning disabled students. (CL)

  11. Biofeedback and Academic Attainment of LD Children.

    ERIC Educational Resources Information Center

    Carter, John L.; Russell, Harold

    1980-01-01

    The study focused on effects of electromyographic (EMG) muscle relaxation training on academic abilities of four learning disabled boys (8 to 13 years old). Ss learned to voluntarily control and decrease forearm muscular tension; and this apparently resulted in an increase in cognitive efficiency, at least as it relates to basic academic areas of…

  12. Transitioning College Bound Students with LD.

    ERIC Educational Resources Information Center

    Brandt, Mary D.; Berry, Judy O.

    1991-01-01

    This article presents a rationale and strategies for providing individualized transition planning for learning-disabled students preparing for postsecondary programs. The article stresses planning and goal setting, academic preparation, personal/social skill development, and student followup. Two case studies illustrate the strategies. (DB)

  13. Complex community of nitrite-dependent anaerobic methane oxidation bacteria in coastal sediments of the Mai Po wetland by PCR amplification of both 16S rRNA and pmoA genes.

    PubMed

    Chen, Jing; Zhou, Zhichao; Gu, Ji-Dong

    2015-02-01

    In the present work, both 16S rRNA and pmoA gene-based PCR primers were employed successfully to study the diversity and distribution of n-damo bacteria in the surface and lower layer sediments at the coastal Mai Po wetland. The occurrence of n-damo bacteria in both the surface and subsurface sediments with high diversity was confirmed in this study. Unlike the two other known n-damo communities from coastal areas, the pmoA gene-amplified sequences in the present work clustered not only with some freshwater subclusters but also within three newly erected marine subclusters mostly, indicating the unique niche specificity of n-damo bacteria in this wetland. Results suggested vegetation affected the distribution and community structures of n-damo bacteria in the sediments and n-damo could coexist with sulfate-reducing methanotrophs in the coastal ecosystem. Community structures of the Mai Po n-damo bacteria based on 16S rRNA gene were different from those of either the freshwater or the marine. In contrast, structures of the Mai Po n-damo communities based on pmoA gene grouped with the marine ones and were clearly distinguished from the freshwater ones. The abundance of n-damo bacteria at this wetland was quantified using 16S rRNA gene PCR primers to be 2.65-6.71 × 10(5) copies/g dry sediment. Ammonium and nitrite strongly affected the community structures and distribution of n-damo bacteria in the coastal Mai Po wetland sediments.

  14. Banana fruit VQ motif-containing protein5 represses cold-responsive transcription factor MaWRKY26 involved in the regulation of JA biosynthetic genes

    PubMed Central

    Ye, Yu-Jie; Xiao, Yun-Yi; Han, Yan-Chao; Shan, Wei; Fan, Zhong-Qi; Xu, Qun-Gang; Kuang, Jian-Fei; Lu, Wang-Jin; Lakshmanan, Prakash; Chen, Jian-Ye

    2016-01-01

    Most harvested fruits and vegetables are stored at low temperature but many of them are highly sensitive to chilling injury. Jasmonic acid (JA), a plant hormone associated with various stress responses, is known to reduce chilling injury in fruits. However, little is known about the transcriptional regulation of JA biosynthesis in relation to cold response of fruits. Here, we show the involvement of a Group I WRKY transcription factor (TF) from banana fruit, MaWRKY26, in regulating JA biosynthesis. MaWRKY26 was found to be nuclear-localized with transcriptional activation property. MaWRKY26 was induced by cold stress or by methyl jasmonate (MeJA), which enhances cold tolerance in banana fruit. More importantly, MaWRKY26 transactivated JA biosynthetic genes MaLOX2, MaAOS3 and MaOPR3 via binding to their promoters. Further, MaWRKY26 physically interacted with a VQ motif-containing protein MaVQ5, and the interaction attenuated MaWRKY26-induced transactivation of JA biosynthetic genes. These results strongly suggest that MaVQ5 might act as a repressor of MaWRKY26 in activating JA biosynthesis. Taken together, our findings provide new insights into the transcriptional regulation of JA biosynthesis in response to cold stress and a better understanding of the molecular aspects of chilling injury in banana fruit. PMID:27004441

  15. HiJaK: the high-resolution J, H and K spectrometer

    NASA Astrophysics Data System (ADS)

    Muirhead, Philip S.; Hall, Zachary J.; Veyette, Mark J.

    2014-08-01

    We present the science drivers, design requirements and a preliminary design for a high-resolution, broad- bandwidth, slit-fed cross-dispersed near-infrared spectrometer for 5-meter-class telescopes. Our concept, called the High-Resolution J, H and K Spectrometer, or HiJaK, utilizes an R6 echelle in a white-pupil design to achieve high resolution in a compact configuration with a 2048 x 2048 pixel infrared detector. We present a preliminary ray-traced optical design matched to the new 4.3-meter Discovery Channel Telescope in Happy Jack, Arizona. We also discuss mechanical and cryogenic options to house our optical design.

  16. High Temperature Induces Expression of Tobacco Transcription Factor NtMYC2a to Regulate Nicotine and JA Biosynthesis

    PubMed Central

    Yang, Liming; Li, Junying; Ji, Jianhui; Li, Ping; Yu, Liangliang; Abd_Allah, Elsayed F.; Luo, Yuming; Hu, Liwei; Hu, Xiangyang

    2016-01-01

    Environmental stress elevates the level of jasmonic acid (JA) and activates the biosynthesis of nicotine and related pyridine alkaloids in tobacco (Nicotiana tabacum L.) by up-regulating the expression of putrescine N-methyltransferase 1 (NtPMT1), which encodes a putrescine N-methyl transferase that catalyzes nicotine formation. The JA signal suppressor JASMONATE ZIM DOMAIN 1 (NtJAZ1) and its target protein, NtMYC2a, also regulate nicotine biosynthesis; however, how these proteins interact to regulate abiotic-induced nicotine biosynthesis is poorly understood. In this study, we found that high-temperature (HT) treatment activated transcription of NtMYC2a, which subsequently stimulated the transcription of genes associated with JA biosynthesis, including Lipoxygenase (LOX), Allene oxide synthase (AOS), Allene oxide cyclase (AOC), and 12-oxophytodienodate reductase (OPR). Overexpression of NtMYC2a increased nicotine biosynthesis by enhancing its binding to the promoter of NtPMT1. Overexpression of either NtJAZ1 or proteasome-resistant NtJAZ1ΔC suppressed nicotine production under normal conditions, but overexpression only of the former resulted in low levels of nicotine under HT treatment. These data suggest that HT induces NtMYC2a accumulation through increased transcription to activate nicotine synthesis; meanwhile, HT-induced NtMYC2a can activate JA synthesis to promote additional NtMYC2a activity by degrading NtJAZ1 at the post-transcriptional level. PMID:27833561

  17. Analysis of Sheng-Mai-San, a Ginseng-Containing Multiple Components Traditional Chinese Herbal Medicine Using Liquid Chromatography Tandem Mass Spectrometry and Physical Examination by Electron and Light Microscopies.

    PubMed

    Cheng, Yung-Yi; Tsai, Tung-Hu

    2016-09-01

    Sheng-Mai-San is a multi-component traditional Chinese herbal preparation. Due to the fact granulated additives, such as starch, carboxymethyl cellulose, lactose and raw herbal powder may alter the content of the bioactive markers in the herbal products, a developed ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method was used to measure the herbal biomarkers of ginsenoside Rb₁, Rb₂, Rc, Rd, Re, Rg₁, Rh₁, compound K, ophiopogonin D and schizandrin from the Sheng-Mai-San herbal formulation. Besides, scanning electron microscopy (SEM) was used to observe the morphology of the herbal granular powders. Light microscopy with Congo red and iodine-KI reagent staining was used to identify the cellulose fiber and cornstarch added to pharmaceutical herbal products. The swelling power (SP), water solubility index (WSI), and crude fiber analysis were used to determine the contents of cellulose fiber and cornstarch in pharmaceutical herbal products. In this study, we developed a novel skill to assess the quantification of appended cornstarch in pharmaceutical herbal products using Aperio ImageScope software. Compared with the traditional cornstarch analysis, our analysis method is a rapid, simple and conversion process which could be applied to detect the percentage of added cornstarch in unknown powder products. The various range of the herbal content for the five pharmaceutical manufacturers varied by up to several hundreds-fold. The physical examination reveals that the morphology of the herbal pharmaceutical products is rough and irregular with sharp layers. This study provides a reference standard operating procedure guide for the quality control of the Chinese herbal pharmaceutical products of Sheng-Mai-San.

  18. Overexpression of OsMYC2 Results in the Up-Regulation of Early JA-Rresponsive Genes and Bacterial Blight Resistance in Rice.

    PubMed

    Uji, Yuya; Taniguchi, Shiduku; Tamaoki, Daisuke; Shishido, Hodaka; Akimitsu, Kazuya; Gomi, Kenji

    2016-09-01

    JASMONATE ZIM-domain (JAZ) proteins act as transcriptional repressors of jasmonic acid (JA) responses and play a crucial role in the regulation of host immunity in plants. Here, we report that OsMYC2, a JAZ-interacting transcription factor in rice (Oryza sativa L.), plays an important role in the resistance response against rice bacterial blight, which is one of the most serious diseases in rice, caused by Xanthomonas oryzae pv. oryzae (Xoo). The results showed that OsMYC2 interacted with some OsJAZ proteins in a JAZ-interacting domain (JID)-dependent manner. The up-regulation of OsMYC2 in response to JA was regulated by OsJAZ8. Transgenic rice plants overexpressing OsMYC2 exhibited a JA-hypersensitive phenotype and were more resistant to Xoo. A large-scale microarray analysis revealed that OsMYC2 up-regulated OsJAZ10 as well as many other defense-related genes. OsMYC2 selectively bound to the G-box-like motif of the OsJAZ10 promoter in vivo and regulated the expression of early JA-responsive genes, but not of late JA-responsive genes. The nuclear localization of OsMYC2 depended on a nuclear localization signal within JID. Overall, we conclude that OsMYC2 acts as a positive regulator of early JA signals in the JA-induced resistance against Xoo in rice.

  19. bHLH003, bHLH013 and bHLH017 Are New Targets of JAZ Repressors Negatively Regulating JA Responses

    PubMed Central

    Fonseca, Sandra; Fernández-Calvo, Patricia; Fernández, Guillermo M.; Díez-Díaz, Monica; Gimenez-Ibanez, Selena; López-Vidriero, Irene; Godoy, Marta; Fernández-Barbero, Gemma; Van Leene, Jelle; De Jaeger, Geert; Franco-Zorrilla, José Manuel; Solano, Roberto

    2014-01-01

    Cell reprogramming in response to jasmonates requires a tight control of transcription that is achieved by the activity of JA-related transcription factors (TFs). Among them, MYC2, MYC3 and MYC4 have been described as activators of JA responses. Here we characterized the function of bHLH003, bHLH013 and bHLH017 that conform a phylogenetic clade closely related to MYC2, MYC3 and MYC4. We found that these bHLHs form homo- and heterodimers and also interact with JAZ repressors in vitro and in vivo. Phenotypic analysis of JA-regulated processes, including root and rosette growth, anthocyanin accumulation, chlorophyll loss and resistance to Pseudomonas syringae, on mutants and overexpression lines, suggested that these bHLHs are repressors of JA responses. bHLH003, bHLH013 and bHLH017 are mainly nuclear proteins and bind DNA with similar specificity to that of MYC2, MYC3 and MYC4, but lack a conserved activation domain, suggesting that repression is achieved by competition for the same cis-regulatory elements. Moreover, expression of bHLH017 is induced by JA and depends on MYC2, suggesting a negative feed-back regulation of the activity of positive JA-related TFs. Our results suggest that the competition between positive and negative TFs determines the output of JA-dependent transcriptional activation. PMID:24465948

  20. JA, a new type of polyunsaturated fatty acid isolated from Juglans mandshurica Maxim, limits the survival and induces apoptosis of heptocarcinoma cells.

    PubMed

    Gao, Xiu-Li; Lin, Hua; Zhao, Wei; Hou, Ya-Qin; Bao, Yong-Li; Song, Zhen-Bo; Sun, Lu-Guo; Tian, Shang-Yi; Liu, Biao; Li, Yu-Xin

    2016-03-01

    Juglans mandshurica Maxim (Juglandaceae) is a famous folk medicine for cancer treatment and some natural compounds isolated from it have been studied extensively. Previously we isolated a type of ω-9 polyunsaturated fatty acid (JA) from the bark of J. mandshurica, however little is known about its activity and the underlying mechanisms. In this study, we studied anti-tumor activity of JA on several human cancer cell lines. Results showed that JA is cytotoxic to HepG2, MDA-MB-231, SGC-7901, A549 and Huh7 cells at a concentration exerting minimal toxic effects on L02 cells. The selective toxicity of JA was better than other classical anti-cancer drugs. Further investigation indicated that JA could induce cell apoptosis, characterized by chromatin condensation, DNA fragmentation and activation of the apoptosis-associated proteins such as Caspase-3 and PARP-1. Moreover, we investigated the cellular apoptosis pathway involved in the apoptosis process in HepG2 cells. We found that proteins involved in mitochondrion (cleaved-Caspase-9, Apaf-1, HtrA2/Omi, Bax, and Mitochondrial Bax) and endocytoplasmic reticulum (XBP-1s, GRP78, cleaved-Caspase-7 and cleaved-Caspase-12) apoptotic pathways were up-regulated when cells were treated by JA. In addition, a morphological change in the mitochondrion was detected. Furthermore, we found that JA could inhibit DNA synthesis and induce G2/M cell cycle arrest. The expression of G2-to-M transition related proteins, such as CyclinB1 and phosphorylated-CDK1, were reduced. In contrast, the G2-to-M inhibitor p21 was increased in JA-treated cells. Overall, our results suggest that JA can induce mitochondrion- and endocytoplasmic reticulum-mediated apoptosis, and G2/M phase arrest in HepG2 cells, making it a promising therapeutic agent against hepatoma.

  1. Simultaneous Qualitative and Quantitative Analysis of Multiple Chemical Constituents in YiQiFuMai Injection by Ultra-Fast Liquid Chromatography Coupled with Ion Trap Time-of-Flight Mass Spectrometry.

    PubMed

    Liu, Chunhua; Ju, Aichun; Zhou, Dazheng; Li, Dekun; Kou, Junping; Yu, Boyang; Qi, Jin

    2016-05-18

    YiQiFuMai injection (YQFM) is a modern lyophilized powder preparation derived from the traditional Chinese medicine Sheng-mai san (SMS) used for treating cardiovascular diseases, such as chronic heart failure. However, its chemical composition has not been fully elucidated, particularly for the preparation derived from Ophiopogon japonicus. This study aimed to establish a systematic and reliable method to quickly and simultaneously analyze the chemical constituents in YQFM by ultra-fast liquid chromatography coupled with ion trap time-of-flight mass spectrometry (UFLC-IT-TOF/MS). Sixty-five compounds in YQFM were tentatively identified by comparison with reference substances or literature data. Furthermore, twenty-one compounds, including three ophiopogonins, fifteen ginsenosides and three lignans were quantified by UFLC-IT-TOF/MS. Notably, this is the first determination of steroidal saponins from O. japonicus in YQFM. The relative standard deviations (RSDs) of intra- and inter-day precision, reproducibility and stability were <4.9% and all analytes showed good linearity (R² ≥ 0.9952) and acceptable recovery of 91.8%-104.2% (RSD ≤ 5.4%), indicating that the methods were reliable. These methods were successfully applied to quantitative analysis of ten batches of YQFM. The developed approach can provide useful and comprehensive information for quality control, further mechanistic studies in vivo and clinical application of YQFM.

  2. Seed germination ecology of feather lovegrass [Eragrostis tenella (L.) Beauv. Ex Roemer & J.A. Schultes].

    PubMed

    Chauhan, Bhagirath S

    2013-01-01

    Feather lovegrass [Eragrostis tenella (L.) Beauv. Ex Roemer & J.A. Schultes] is a C4 grass weed that has the ability to grow in both lowland and upland conditions. Experiments were conducted in the laboratory and screenhouse to evaluate the effect of environmental factors on germination, emergence, and growth of this weed species. Germination in the light/dark regime was higher at alternating day/night temperatures of 30/20 °C (98%) than at 35/25 °C (83%) or 25/15 °C (62%). Germination was completely inhibited by darkness. The osmotic potential and sodium chloride concentrations required for 50% inhibition of maximum germination were -0.7 MPa and 76 mM, respectively. The highest seedling emergence (69%) was observed from the seeds sown on the soil surface and no seedlings emerged from seeds buried at depths of 0.5 cm or more. The use of residue as mulches significantly reduced the emergence and biomass of feather lovegrass seedlings. A residue amount of 0.5 t ha(-1) was needed to suppress 50% of the maximum seedlings. Because germination was strongly stimulated by light and seedling emergence was the highest for the seeds sown on the soil surface, feather lovegrass is likely to become a problematic weed in zero-till systems. The knowledge gained from this study could help in developing effective and sustainable weed management strategies.

  3. High-Rate Mechanical Properties of JA2 Propellant at Temperatures from -50 to 80 deg C

    DTIC Science & Technology

    2015-07-01

    Joyce E Newberry 5d. PROJECT NUMBER 622618.H8000 5e. TASK NUMBER 5f. WORK UNIT NUMBER 7. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) US...Army Research Laboratory ATTN: RDRL-WML-D Aberdeen Proving Ground, MD 21005-5069 8. PERFORMING ORGANIZATION REPORT NUMBER ARL-MR-0894 9...AVAILABILITY STATEMENT Approved for public release; distribution is unlimited. 13. SUPPLEMENTARY NOTES 14. ABSTRACT JA2, a viscoelastic polymeric

  4. 40 CFR Table 1 to Subpart Ja of... - Molar Exhaust Volumes and Molar Heat Content of Fuel Gas Constituents

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Content of Fuel Gas Constituents 1 Table 1 to Subpart Ja of Part 60 Protection of Environment... Exhaust Volumes and Molar Heat Content of Fuel Gas Constituents Constituent MEVa dscf/mol MHCb Btu/mol Methane (CH4) 7.29 842 Ethane (C2H6) 12.96 1,475 Hydrogen (H2) 1.61 269 Ethene (C2H4) 11.34 1,335...

  5. Visualization and Measurement of the Burning Surface of Wire-Embedded Energetic Materials, Part 1: JA2 and Pentolite

    DTIC Science & Technology

    2014-06-01

    figure 4). The apparatus includes a windowed chamber capable of being pressurized up to 10 MPa. It also includes a ballast tank that adds considerably... pressures were acquired, and they can be employed to validate a state-of-the-art CFD model that ARL is developing to accelerate the development of...rates at pressure near 5.18 MPa. ..............................................................12 Figure 11. JA2 burning rates at 6.90 MPa

  6. A Cursive Handwriting Skills Program for LD Students To Be Used by Regular and LD Teachers.

    ERIC Educational Resources Information Center

    McMillan, Ida L.

    Many learning disabled students attending Avocado Elementary School in Homestead, Florida, were unable to write legibly when taught with available cursive handwriting programs. To redress the problem, a complete, sequential cursive handwriting program was devised for use with learning disabled and other students. The program combined tracing and…

  7. Diverting the flux of the JA pathway in Nicotiana attenuata compromises the plant's defense metabolism and fitness in nature and glasshouse.

    PubMed

    Stitz, Michael; Baldwin, Ian T; Gaquerel, Emmanuel

    2011-01-01

    A plant's inducible defenses against herbivores as well as certain developmental processes are known to be controlled by the jasmonic acid (JA) pathway. We have previously shown that ectopically expressing Arabidopsis thaliana JA O-methyltransferase in Nicotiana attenuata (35S-jmt) strongly reduces the herbivory-elicited jasmonate bursts by acting as metabolic sink that redirects free JA towards methylation; here we examine the consequences of this metabolic sink on N. attenuata's secondary metabolism and performance in nature. In the glasshouse, 35S-jmt plants produced fewer seed capsules due to shorter floral styles, which could be restored to wild type (WT) levels after hand-pollination, and were more susceptible to Manduca sexta larvae attack. When transplanted into the Great Basin Desert in Utah, 35S-jmt plants grew as well as WT empty vector, but were highly attacked by native herbivores of different feeding guilds: leaf chewers, miners, and single cell feeders. This greater susceptibility was strongly associated with reduced emissions of volatile organic compounds (hexenylesters, monoterpenes and sesquiterpenes) and profound alterations in the production of direct defenses (trypsin proteinase inhibitors [TPI], nicotine, diterpene glycosides [DTGs] and phenylpropanoid-polyamine conjugates) as revealed by a combination of targeted and metabolomics analyses of field collected samples. Complementation experiments with JA-Ile, whose formation is outcompeted in 35S-jmt plants by the methylation reaction, restored the local TPI activation to WT levels and partially complemented nicotine and DTG levels in elicited but not systemic leaves. These findings demonstrate that MeJA, the major JA metabolite in 35S-jmt plants, is not an active signal in defense activation and highlights the value of creating JA sinks to disrupt JA signaling, without interrupting the complete octadecanoid pathway, in order to investigate the regulation of plants' defense metabolism in nature.

  8. Determination of Two-Liquid Mixture Composition by Assessing Dielectric Parameters 1. Precise Measuring System / Divu Šķidrumu Maisījuma Sastāva Noteikšana, Izvērtējot to Dielektriskos Parametrus 1. Precīza Mērīšanas Sistēma

    NASA Astrophysics Data System (ADS)

    Vilitis, O.; Shipkovs, P.; Merkulovs, D.

    2013-08-01

    Concentration measurements are important in bioethanol industries, in the R&D areas, for chemical, medical and microbiological analyses and processing as well as for diagnostics, manufacturing, etc. The overview shows development of the structural design of a system for measuring the concentration of solutions and mixtures consisting of two dielectric liquids. The basic principles of the system's design are given along with relevant equations. The concentration of dielectric liquids is measured using devices with capacitive sensors (1-300 pF). The operational frequency of the developed measuring system is 100.000 kHz. Configuration of the system excludes some errors usually arising at measurements, and broadens its applicability. For testing, the system was calibrated for measuring the concentration of anhydrous ethanol + de-ionized water mixture. Experimental results have shown a stable resolution of ±0.005 pF at measuring the sensor capacitance and a reproducible resolution better than ±0.01% at measuring the ethanol volume concentration Rakstā esam parādījuši iespējas izveidot augstas precizitātes, kompaktu, lētu un ērtu lietošanai dielektrisku šķidrumu mērīšanas sistēmu koncentrācijas noteikšanai. Šī sistēma ir piemērojama kapacitīviem sensoriem, kuru kapacitāte ir atkarīga no sensora izveidojuma kā arī mērāmā šķidruma dielektriskās konstantes vērtības, un kapacitāte var tikt noteikta pie frekvences 100,000 kHz robežās no 1 F līdz 300 pF. Mērīšanas sistēmas pārbaudei, sistēma tika kalibrēta etanola koncentrācijas mērīšanai tilpuma procentos sertificēta bezūdens etanola un dejonizēta ūdens maisījumiem. Pārbaužu rezultāti pierādīja, ka sensora kapacitātes vērtības ir stabili nosakāmas ar izšķirtspēju ne mazāku par ±0,005 pF. Sensora kapacitāšu vērtībām atbilstošā etanola tilpuma koncentrācijas atkārtojamu mērījumu izšķirtspēja visā mērīšanas diapazonā nebija mazāka par ±0

  9. A bHLH-Type Transcription Factor, ABA-INDUCIBLE BHLH-TYPE TRANSCRIPTION FACTOR/JA-ASSOCIATED MYC2-LIKE1, Acts as a Repressor to Negatively Regulate Jasmonate Signaling in Arabidopsis[C][W

    PubMed Central

    Nakata, Masaru; Mitsuda, Nobutaka; Herde, Marco; Koo, Abraham J.K.; Moreno, Javier E.; Suzuki, Kaoru; Howe, Gregg A.; Ohme-Takagi, Masaru

    2013-01-01

    Jasmonates (JAs) are plant hormones that regulate the balance between plant growth and responses to biotic and abiotic stresses. Although recent studies have uncovered the mechanisms for JA-induced responses in Arabidopsis thaliana, the mechanisms by which plants attenuate the JA-induced responses remain elusive. Here, we report that a basic helix-loop-helix–type transcription factor, ABA-INDUCIBLE BHLH-TYPE TRANSCRIPTION FACTOR/JA-ASSOCIATED MYC2-LIKE1 (JAM1), acts as a transcriptional repressor and negatively regulates JA signaling. Gain-of-function transgenic plants expressing the chimeric repressor for JAM1 exhibited substantial reduction of JA responses, including JA-induced inhibition of root growth, accumulation of anthocyanin, and male fertility. These plants were also compromised in resistance to attack by the insect herbivore Spodoptera exigua. Conversely, jam1 loss-of-function mutants showed enhanced JA responsiveness, including increased resistance to insect attack. JAM1 and MYC2 competitively bind to the target sequence of MYC2, which likely provides the mechanism for negative regulation of JA signaling and suppression of MYC2 functions by JAM1. These results indicate that JAM1 negatively regulates JA signaling, thereby playing a pivotal role in fine-tuning of JA-mediated stress responses and plant growth. PMID:23673982

  10. Etat nutritionnel des enfants âgés de 6 à 59 mois infectés par le VIH mais non traités aux ARV à Lubumbashi

    PubMed Central

    Mwadianvita, Costa Kazadi; Kanyenze, Faustin Ngoy; Wembonyama, Cecile Watu; Mutomb, Florence Mujing A; Mupoya, Kalombo; Nkoy, Albert Mwembo–Tambwe A; Mwenze, Prosper Kalenga

    2014-01-01

    Introduction L'infection par le VIH provoque et/ou aggrave les déficits nutritionnels de l'enfant. Ce travail avait pour objectif d'analyser l’état nutritionnel des enfants infectés par le VIH à Lubumbashi. Méthodes Une étude transversale portant sur 83 enfants âgés de 6 à 60 mois s'est déroulée de mai 2010 à mai 2011 dans trois(3) centres de prise en charge des Personnes Vivant avec le VIH(PVV), notamment le Centre d'Excellence(CE) de l'hôpital Sendwe, le Centre Amo-Congo de la Kenya et le Centre de Référence de la Kenya. Les statistiques descriptives usuelles ont été utilisées. Résultats La prévalence de la malnutrition globale était de 60,2% (n = 50) dont 8,4% de malnutrition sévère. Le poids moyen était de 11,6±4,1 kg avec un minimum de 5 kg et un maximum de 22 kg. Le taux d'hémoglobine moyen était d'environ 9,8± 2,0 g/dl avec une prévalence globale de l'anémie (hémoglobine < 11g/dl) à 69,9%. L’émaciation concernait 20,5% des enfants et 8,4% avaient un retard de croissance. Le retard de croissance (p = 0,007), l'insuffisance pondérale (p = 0,002) et l’émaciation (p = 0,046) étaient associés de façon significative à l’état avancé de l'infection à VIH. La survenue de l'anémie n’était pas associée au déficit nutritionnel (p = 0,6). Conclusion Ces résultats révèlent que l'infection à VIH modifie l’état nutritionnel des enfants à Lubumbashi avec 60,2% de malnutrition globale et 8,4% de retard de croissance. Les enfants au stade avancé de l'infection à VIH en sont plus affectés. PMID:25574336

  11. Analysis of methane-producing and metabolizing archaeal and bacterial communities in sediments of the northern South China Sea and coastal Mai Po Nature Reserve revealed by PCR amplification of mcrA and pmoA genes

    PubMed Central

    Zhou, Zhichao; Chen, Jing; Cao, Huiluo; Han, Ping; Gu, Ji-Dong

    2015-01-01

    Communities of methanogens, anaerobic methanotrophic archaea and aerobic methanotrophic bacteria (MOB) were compared by profiling polymerase chain reaction (PCR)-amplified products of mcrA and pmoA genes encoded by methyl-coenzyme M reductase alpha subunit and particulate methane monooxygenase alpha subunit, respectively, in sediments of northern South China Sea (nSCS) and Mai Po mangrove wetland. Community structures representing by mcrA gene based on 12 clone libraries from nSCS showed separate clusters indicating niche specificity, while, Methanomicrobiales, Methanosarcinales clades 1,2, and Methanomassiliicoccus-like groups of methanogens were the most abundant groups in nSCS sediment samples. Novel clusters specific to the SCS were identified and the phylogeny of mcrA gene-harboring archaea was updated. Quantitative polymerase chain reaction was used to detect mcrA gene abundance in all samples: similar abundance of mcrA gene in the surface layers of mangrove (3.4∼3.9 × 106 copies per gram dry weight) and of intertidal mudflat (5.5∼5.8 × 106 copies per gram dry weight) was observed, but higher abundance (6.9 × 106 to 1.02 × 108 copies per gram dry weight) was found in subsurface samples of both sediment types. Aerobic MOB were more abundant in surface layers (6.7∼11.1 × 105 copies per gram dry weight) than the subsurface layers (1.2∼5.9 × 105 copies per gram dry weight) based on pmoA gene. Mangrove surface layers harbored more abundant pmoA gene than intertidal mudflat, but less pmoA genes in the subsurface layers. Meanwhile, it is also noted that in surface layers of all samples, more pmoA gene copies were detected than the subsurface layers. Reedbed rhizosphere exhibited the highest gene abundance of mcrA gene (8.51 × 108 copies per gram dry weight) and pmoA gene (1.56 × 107 copies per gram dry weight). This study investigated the prokaryotic communities responsible for methane cycling in both marine and coastal wetland ecosystems, showing

  12. Temporal frequency of knockdown resistance mutations, F1534C and V1016G, in Aedes aegypti in Chiang Mai city, Thailand and the impact of the mutations on the efficiency of thermal fogging spray with pyrethroids.

    PubMed

    Plernsub, Suriya; Saingamsook, Jassada; Yanola, Jintana; Lumjuan, Nongkran; Tippawangkosol, Pongsri; Walton, Catherine; Somboon, Pradya

    2016-10-01

    In Thailand, control of dengue outbreaks is currently attained by the use of space sprays, particularly thermal fogging using pyrethroids, with the aim of killing infected Aedes mosquito vectors in epidemic areas. However, the principal dengue vector, Aedes aegypti, is resistant to pyrethroids conferred mainly by mutations in the voltage-gated sodium channel gene, F1534C and V1016G, termed knockdown resistance (kdr). The objectives of this study were to determine the temporal frequencies of F1534C and V1016G in Ae. aegypti populations in relation to pyrethroid resistance in Chiang Mai city, and to evaluate the impact of the mutations on the efficacy of thermal fogging with the pyrethroid deltamethrin. Larvae and pupae were collected from several areas around Chiang Mai city during 2011-2015 and reared to adulthood for bioassays for deltamethrin susceptibility. These revealed no trend of increasing deltamethrin resistance during the study period (mortality 58.0-69.5%, average 62.8%). This corresponded to no overall change in the frequencies of the C1534 allele (0.55-0.66, average 0.62) and G1016 allele (0.34-0.45, average 0.38), determined using allele specific amplification. Only three genotypes of kdr mutations were detected: C1534 homozygous (VV/CC); G1016/C1534 double heterozygous (VG/FC); and G1016 homozygous (GG/FF) indicating that the F1534C and V1016G mutations occurred on separate haplotypic backgrounds and a lack of recombination between them to date. The F1 progeny females were used to evaluate the efficacy of thermal fogging spray with Damthrin-SP(®) (deltamethrin+S-bioallethrin+piperonyl butoxide) using a caged mosquito bioassay. The thermal fogging spray killed 100% and 61.3% of caged mosquito bioassay placed indoors and outdoors, respectively. The outdoor spray had greater killing effect on C1534 homozygous and had partially effect on double heterozygous mosquitoes, but did not kill any G1016 homozygous mutants living outdoors. As this selection

  13. OsMYC2, an essential factor for JA-inductive sakuranetin production in rice, interacts with MYC2-like proteins that enhance its transactivation ability

    PubMed Central

    Ogawa, Satoshi; Miyamoto, Koji; Nemoto, Keiichirou; Sawasaki, Tatsuya; Yamane, Hisakazu; Nojiri, Hideaki; Okada, Kazunori

    2017-01-01

    Biosynthesis of sakuranetin, a flavonoid anti-fungal phytoalexin that occurs in rice, is highly dependent on jasmonic acid (JA) signalling and induced by a variety of environmental stimuli. We previously identified OsNOMT, which encodes naringenin 7-O-methyltransferase (NOMT); NOMT is a key enzyme for sakuranetin production. Although OsNOMT expression is induced by JA treatment, the regulation mechanism that activates the biosynthetic pathway of sakuranetin has not yet been elucidated. In this study, we show that JA-inducible basic helix-loop-helix transcriptional factor OsMYC2 drastically enhances the activity of the OsNOMT promoter and is essential for JA-inducible sakuranetin production. In addition, we identified 2 collaborators of OsMYC2, OsMYC2-like protein 1 and 2 (OsMYL1 and OsMYL2) that further activated the OsNOMT promoter in synergy with OsMYC2. Physical interaction of OsMYC2 with OsMYL1 and OsMYL2 further supported the idea that these interactions lead to the enhancement of the transactivation activity of OsMYC2. Our results indicate that JA signalling via OsMYC2 is reinforced by OsMYL1 and OsMYL2, resulting in the inductive production of sakuranetin during defence responses in rice. PMID:28067270

  14. Dental fluorosis in populations from Chiang Mai, Thailand with different fluoride exposures - Paper 2: The ability of fluorescence imaging to detect differences in fluorosis prevalence and severity for different fluoride intakes from water

    PubMed Central

    2012-01-01

    Background To assess the ability of fluorescence imaging to detect a dose response relationship between fluorosis severity and different levels of fluoride in water supplies compared to remote photographic scoring in selected populations participating in an observational, epidemiological survey in Chiang Mai, Thailand. Methods Subjects were male and female lifetime residents aged 8-13 years. For each child the fluoride content of cooking water samples (CWS) was assessed to create categorical intervals of water fluoride concentration. Fluorescence images were taken of the maxillary central incisors and analyzed for dental fluorosis using two different software techniques. Output metrics for the fluorescence imaging techniques were compared to TF scores from blinded photographic scores obtained from the survey. Results Data from 553 subjects were available. Both software analysis techniques demonstrated significant correlations with the photographic scores. The metrics for area effected by fluorosis and the overall fluorescence loss had the strongest association with the photographic TF score (Spearman’s rho 0.664 and 0.652 respectively). Both software techniques performed well for comparison of repeat fluorescence images with ICC values of 0.95 and 0.85 respectively. Conclusions This study supports the potential use of fluorescence imaging for the objective quantification of dental fluorosis. Fluorescence imaging was able to discriminate between populations with different fluoride exposures on a comparable level to remote photographic scoring with acceptable levels of repeatability. PMID:22908997

  15. Qualitative and quantitative analysis of multiple components for quality control of Deng-Zhan-Sheng-Mai capsules by ultra high performance liquid chromatography tandem mass spectrometry method coupled with chemometrics.

    PubMed

    Jiang, Pin; Lu, Yan; Chen, Daofeng

    2017-02-01

    Deng-Zhan-Sheng-Mai capsules are a well-known traditional Chinese patent medicine that was developed in China for the treatment of ischemic stroke. Its quality control focuses on Erigerontis Herba but ignores the contributions of Ginseng Radix et Rhizoma, Schisandrae Chinensis Fructus, and Ophiopogonis Radix. To improve the quality standards for this medicine, this work reports the application of a systematic ultra high performance liquid chromatography tandem mass spectrometric method coupled with chemometrics. Three qualitative and quantitative parameters are established for the evaluation of quality: chemical profiling, the relationship between the contents of 18 compounds and the antioxidant activity, and chemometric analysis. A total of 55 compounds, including 20 phenolic acids, 10 flavonoids, 15 saponins, and 10 lignans, were identified. The method for the quantitative determination of the aforementioned 18 compounds was validated. The limit of quantification ranged from 0.13 to 9.60 ng/mL. The overall recoveries ranged from 95.31 to 103.54%. Hierarchical cluster analysis and principal component analysis were applied to the data of 18 components in ten batches of samples. Nine compounds, including scutellarin, 3,5-O-dicaffeoylquinic acid, 4,5-O-dicaffeoylquinic acid, ginsenoside Rb1, ginsenoside Re, ginsenoside Rg1, ophiopogonin D, schisandrin, and schisandrol B, are suggested as chemical markers for evaluating the quality.

  16. The Combination of Three Components Derived from Sheng MaiSan Protects Myocardial Ischemic Diseases and Inhibits Oxidative Stress via Modulating MAPKs and JAK2-STAT3 Signaling Pathways Based on Bioinformatics Approach

    PubMed Central

    Li, Fang; Zhang, Yu; Zeng, Donglin; Xia, Yu; Fan, Xiaoxue; Tan, Yisha; Kou, Junping; Yu, Boyang

    2017-01-01

    GRS is a drug combination of three components including ginsenoside Rb1, ruscogenin and schisandrin. It derived from the well-known TCM formula Sheng MaiSan, a widely used traditional Chinese medicine for the treatment of cardiovascular diseases in clinic. The present study illuminates its underlying mechanisms against myocardial ischemic diseases based on the combined methods of bioinformatic prediction and experimental verification. A protein database was established through constructing the drug-protein network. And the target-pathway interaction network clustered the potential signaling pathways and targets of GRS in treatment of myocardial ischemic diseases. Several target proteins, such as NFKB1, STAT3 and MAPK14, were identified as the candidate key proteins, and MAPKs and JAK-STAT signaling pathway were suggested as the most related pathways, which were in accordance with the gene ontology analysis. Then, the predictive results were further validated and we found that GRS treatment alleviated hypoxia/reoxygenation (H/R)-induced cardiomyocytes injury via suppression of MDA levels and ROS generation, and potential mechanisms might related to the suppression of activation of MAPKs and JAK2-STAT3 signaling pathways. Conclusively, our results offer the evidence that GRS attenuates myocardial ischemia injury via regulating oxidative stress and MAPKs and JAK2-STAT3 signaling pathways, which supplied some new insights for its prevention and treatment of myocardial ischemia diseases. PMID:28197101

  17. Expanding health access in the more vulnerable region in the state of São Paulo, Brazil: is this a reflection of the Mais Médicos (More Doctors) Program?

    PubMed

    Silva, Bruna Pontes da; Stockmann, Denise; Lúcio, Donavan de Souza; Henna, Elaine; Rocha, Maria Carolina Pereira da; Junqueira, Fábio Miranda

    2016-09-01

    The Mais Médicos (More Doctors) Program seeks to broaden access to health by providing medical professionals, investments in health units and multi-professional integration geared to the Family Health Strategy. Vale do Ribeira includes 25 cities and is among the most vulnerable regions in São Paulo. It has been allocated 41 physicians from the Program. This study is to evaluate access to health, comparing health indicators before and after the Program. We collected data from DATASUS, SIAB, and the Ministry of Health. There was a marked increase in the number of appointments for infants under one year of age, adults, the elderly, STD/HIV patients and group patient care. There was a decrease in appointments outside the catchment area, as well as hospital admissions for other causes, mothers exclusively breastfeeding their infants up to four months. We concluded that after deployment of the Program, there was an increase in health access and health promotion focused on an area that presents an enormous challenge for Primary Health Care (PHC). It would seem that, since this is a high vulnerability area with a large area for care, hospital admissions for PHC care-sensitive conditions, as well as referrals for secondary services, did not decrease.

  18. JaTS: a fully portable seismic tomography software based on Fresnel wavepaths and a probabilistic reconstruction approach

    NASA Astrophysics Data System (ADS)

    Grandjean, Gilles; Sage, Sandrine

    2004-11-01

    JaTS, a Java 2D seismic tomography software, is presented. It implements original algorithms achieving optimal accuracy with reasonable computing costs. A second-order Fast Marching Method (FMM) is used for solving the eikonal equation, therefore enabling a fast and robust computation of seismic traveltimes between sources and receivers. The wavepaths are materialized by Fresnel volumes rather than by conventional rays. This approach accounts for complex velocity models and has the advantage of considering the effects of the wave frequency in the velocity model resolution. The model is computed by a Simultaneous Iterative Reconstruction Technique (SIRT) which has been reformulated to integrate Fresnel wavepaths by using a probabilistic approach. In addition, various utilities are implemented, such as a tapering filter, used to decrease artifact effects occurring in the vicinity of the sources and receivers. The software also offers the possibility of reconstructing the velocity field on a grid larger than the one used for the wave propagation computation. This contributes to stabilize the estimated values. All of the seismic processing tools have been integrated with a user-friendly graphical interface. JaTS represents a tightly integrated tool suite that supports the entire process of importing the SG2 field records, first-break picking, forward modeling and velocity-field computing across multiple platforms.

  19. A combination of four effective components derived from Sheng-mai san attenuates hydrogen peroxide-induced injury in PC12 cells through inhibiting Akt and MAPK signaling pathways.

    PubMed

    Cao, Guo-Sheng; Li, Shao-Xia; Wang, Yan; Xu, Ying-Qiong; Lv, Yan-Ni; Kou, Jun-Ping; Yu, Bo-Yang

    2016-07-01

    The present study was designed to investigate whether a combination of four effective components derived from Sheng-mai san (SMXZF; ginsenoside Rb1: ginsenoside Rg1: DT-13: Schizandrol A as 6 : 9 : 4 : 5) could attenuate hydrogen peroxide (H2O2)-induced injury in PC12 cells, focusing on the Akt and MAPK pathways . The PC12 cells were exposed to H2O2 (400 μmol·L(-1)) for 1 h in the presence or absence of SMXZF pre-treatment for 24 h. Cell viability was measured by MTT assay. The efflux of lactate dehydrogenase (LDH), the intracellular content of malondialdehyde (MDA), the activities of superoxide dismutase (SOD), and caspase-3 were also determined. Cell apoptosis was measured by Hoechst 33342 staining and Annexin V-FITC/PI staining method. The expression of Bcl-2, Bax, cleaved caspase-3, Akt, and MAPKs were detected by Western blotting analyses. SMXZF pretreatment significantly increased the cell viability and SOD activity and improved the cell morphological changes, while reduced the levels of LDH and MDA at the concentrations of 0.1, 1 and 10 μg·mL(-1). SMXZF also inhibited H2O2-induced apoptosis in PC12 cells. Moreover, SMXZF reduced the activity of caspase-3, up-regulated the protein ratio of Bcl-2 and Bax and inhibited the expression of cleaved caspase-3, p-Akt, p-p38, p-JNK and p-ERK1/2 in H2O2-induced PC12 cells. Co-incubation of Akt inhibitor or p38 inhibitor partly attenuated the protection of SMXZF against H2O2-injured PC12 cells. In conclusion, our findings suggested that SMXZF attenuated H2O2-induced injury in PC12 cells by inhibiting Akt and MAPKs signaling pathways, which might shed insights on its neuroprotective mechanism.

  20. Effects of Sheng-Mai injection on the PRPP synthetase activity in BFU-es and CFU-es from bone marrows of mice with benzene-induced aplastic anemia.

    PubMed

    Liu, L P; Liu, J F; Lu, Y Q

    2001-08-10

    160 Kunming mice were divided at random into 3 groups. Group 1: normal control (40 mice). Group 2: aplastic anemia (AA) control (60 mice); benzene inhalation was carried out for 2.5 months and sterilized normal saline was injected i.p. for another 6 weeks. Group 3: treated AA (60 mice); benzene was administered by inhalation in a similar manner, Sheng-Mai Injection (SMI) was administered i.p. for 6 weeks after the AA models were established. SMI is a famous Chinese traditional prescription of Panax ginseng C.A. Meyer (0.1 g/ml), Ophiopogon japonicus (Thunb.) Ker-Gawl (0.312 g/ml) and Fructus Schisandrae (0.158 g/ml). Activities of phosphoribosylpyrophosphate (PRPP) synthetase in BFU-Es and CFU-Es were estimated by ion pair reversed phase HPLC (IPrHPLC). Accompanying the sharp drop in counts of erythroid progenitor cells, the PRPP synthetase activity in CFU-Es of AA mice was reduced significantly (P<0.01), whereas there were no remarkable changes of this enzyme activity in their BFU-Es compared with the control group. Both the counts of erythroid progenitor cells and PRPP synthetase activity in CFU-Es returned nearly to normal levels following treatment with SMI of mice in Group 3 (P<0.01). Our results suggest that the attenuation of PRPP synthetase activity in peripheral erythrocytes of AA patients may originate from the weakening of activity of this enzyme in CFU-Es from their bone marrow. The impairment of PRPP formation would explain ATP depletion and disorders of energy metabolism in AA erythrocytes. SMI can distinctly increase the reduced quantity of erythroid progenitor cells and promote rapid restoration of PRPP synthetase activity in CFU-Es of AA mice.

  1. Integrated Performance of Next Generation High Data Rate Receiver and AR4JA LDPC Codec for Space Communications

    NASA Technical Reports Server (NTRS)

    Cheng, Michael K.; Lyubarev, Mark; Nakashima, Michael A.; Andrews, Kenneth S.; Lee, Dennis

    2008-01-01

    Low-density parity-check (LDPC) codes are the state-of-the-art in forward error correction (FEC) technology that exhibits capacity approaching performance. The Jet Propulsion Laboratory (JPL) has designed a family of LDPC codes that are similar in structure and therefore, leads to a single decoder implementation. The Accumulate-Repeat-by-4-Jagged- Accumulate (AR4JA) code design offers a family of codes with rates 1/2, 2/3, 4/5 and lengths 1024, 4096, 16384 information bits. Performance is less than one dB from capacity for all combinations.Integrating a stand-alone LDPC decoder with a commercial-off-the-shelf (COTS) receiver faces additional challenges than building a single receiver-decoder unit from scratch. In this work, we outline the issues and show that these additional challenges can be over-come by simple solutions. To demonstrate that an LDPC decoder can be made to work seamlessly with a COTS receiver, we interface an AR4JA LDPC decoder developed on a field-programmable gate array (FPGA) with a modern high data rate receiver and mea- sure the combined receiver-decoder performance. Through optimizations that include an improved frame synchronizer and different soft-symbol scaling algorithms, we show that a combined implementation loss of less than one dB is possible and therefore, most of the coding gain evidence in theory can also be obtained in practice. Our techniques can benefit any modem that utilizes an advanced FEC code.

  2. The MeJA-inducible copper amine oxidase AtAO1 is expressed in xylem tissue and guard cells.

    PubMed

    Ghuge, Sandip A; Carucci, Andrea; Rodrigues-Pousada, Renato A; Tisi, Alessandra; Franchi, Stefano; Tavladoraki, Paraskevi; Angelini, Riccardo; Cona, Alessandra

    2015-01-01

    Copper amine oxidases oxidize the polyamine putrescine to 4-aminobutanal with the production of the plant signal molecule hydrogen peroxide (H2O2) and ammonia. The Arabidopsis (Arabidopsis thaliana) gene At4g14940 (AtAO1, previously referred to as ATAO1) encodes an apoplastic copper amine oxidase expressed in lateral root cap cells and developing xylem, especially in root protoxylem and metaxylem precursors. In our recent study, we demonstrated that AtAO1 expression is strongly induced in the root vascular tissues by the wound-signal hormone methyl jasmonate (MeJA). Furthermore, we also demonstrated that the H2O2 derived by the AtAO1-driven oxidation of putrescine, mediates the MeJA-induced early protoxylem differentiation in Arabidopsis roots. H2O2 may contribute to protoxylem differentiation by signaling developmental cell death and by acting as co-substrate in peroxidase-mediated cell wall stiffening and lignin polymerization. Here, by the means of AtAO1 promoter::green fluorescent protein-β-glucuronidase (AtAO1::GFP-GUS) fusion analysis, we show that a strong AtAO1 gene expression occurs also in guard cells of leaves and flowers. The high expression levels of AtAO1 in tissues or cell types regulating water supply and water loss may suggest a role of the encoded protein in water balance homeostasis, by modulating coordinated adjustments in anatomical and functional features of xylem tissue and guard cells during acclimation to adverse environmental conditions.

  3. Structural basis of jasmonate-amido synthetase FIN219 in complex with glutathione S-transferase FIP1 during the JA signal regulation

    PubMed Central

    Chen, Chun-Yen; Ho, Sih-Syun; Kuo, Tzu-Yen; Cheng, Yi-Sheng

    2017-01-01

    Far-red (FR) light-coupled jasmonate (JA) signaling is necessary for plant defense and development. FR insensitive 219 (FIN219) is a member of the Gretchen Hagen 3 (GH3) family of proteins in Arabidopsis and belongs to the adenylate-forming family of enzymes. It directly controls biosynthesis of jasmonoyl-isoleucine in JA-mediated defense responses and interacts with FIN219-interacting protein 1 (FIP1) under FR light conditions. FIN219 and FIP1 are involved in FR light signaling and are regulators of the interplay between light and JA signaling. However, how their interactions affect plant physiological functions remains unclear. Here, we demonstrate the crystal structures of FIN219–FIP1 while binding with substrates at atomic resolution. Our results show an unexpected FIN219 conformation and demonstrate various differences between this protein and other members of the GH3 family. We show that the rotated C-terminal domain of FIN219 alters ATP binding and the core structure of the active site. We further demonstrate that this unique FIN219–FIP1 structure is crucial for increasing FIN219 activity and determines the priority of substrate binding. We suggest that the increased FIN219 activity resulting from the complex form, a conformation for domain switching, allows FIN219 to switch to its high-affinity mode and thereby enhances JA signaling under continuous FR light conditions. PMID:28223489

  4. MdMYB9 and MdMYB11 are involved in the regulation of the JA-induced biosynthesis of anthocyanin and proanthocyanidin in apples.

    PubMed

    An, Xiu-Hong; Tian, Yi; Chen, Ke-Qin; Liu, Xiao-Juan; Liu, Dan-Dan; Xie, Xing-Bin; Cheng, Cun-Gang; Cong, Pei-Hua; Hao, Yu-Jin

    2015-04-01

    Anthocyanin and proanthocyanidin (PA) are important secondary metabolites and beneficial to human health. Their biosynthesis is induced by jasmonate (JA) treatment and regulated by MYB transcription factors (TFs). However, which and how MYB TFs regulate this process is largely unknown in apple. In this study, MdMYB9 and MdMYB11 which were induced by methyl jasmonate (MeJA) were functionally characterized. Overexpression of MdMYB9 or MdMYB11 promoted not only anthocyanin but also PA accumulation in apple calluses, and the accumulation was further enhanced by MeJA. Subsequently, yeast two-hybrid, pull-down and bimolecular fluorescence complementation assays showed that both MYB proteins interact with MdbHLH3. Moreover, Jasmonate ZIM-domain (MdJAZ) proteins interact with MdbHLH3. Furthermore, chromatin immunoprecipitation-quantitative PCR and yeast one-hybrid assays demonstrated that both MdMYB9 and MdMYB11 bind to the promoters of ANS, ANR and LAR, whereas MdbHLH3 is recruited to the promoters of MdMYB9 and MdMYB11 and regulates their transcription. In addition, transient expression assays indicated that overexpression of MdJAZ2 inhibits the recruitment of MdbHLH3 to the promoters of MdMYB9 and MdMYB11. Our findings provide new insight into the mechanism of how MeJA regulates anthocyanin and PA accumulation in apple.

  5. The pharmacokinetic screening of multiple components of the Nao Mai Tong formula in rat plasma by liquid chromatography tandem mass spectrometry combined with pattern recognition method and its application to comparative pharmacokinetics.

    PubMed

    Wu, Chunwei; Zhao, Lu; Rong, Yueying; Zhu, Guoxue; Liang, Shengwang; Wang, Shumei

    2016-11-30

    The Nao Mai Tong formula (NMT) is composed of Rhubarb, Ginseng, Ligusticum wallichii and Pueraria in a ratio of 3:3:2:2 (w/w) and is a well-known traditional Chinese prescription that has been clinically employed for treating ischemia cerebrovascular disease. The goal of this study was to investigate the pharmacokinetics of multiple components (chryohol-8-O-β-D-glucoyroide, physcion-8-O-β-D-glucopyranoside, aloe-emodin, rhein, emodin, chrysophanol, ginsenoside Rg1, ginsenoside Rb1, ginsenoside Rb3, ginsenoside Rc, senkyunolide I, ligustilide puerarin, daidzein, 3'-methoxy puerarin) after the oral administration of the NMT formula in rats. A rapid and sensitive UHPLC-Quadrupole-Orbitrap-MS with a sequential positive and negative ionization mode was developed to determine the 15 absorbed ingredients. After extraction from blood, the analytes and internal standards were subjected to ultra-high performance liquid chromatography with Agela Venusil MPC18 (2.1mm×100mm, 3μm, Agela, USA). The mobile phase consisted of methanol and ammonium acetate (3mmolL(-1)) under gradient elution conditions. This validated method was successfully applied to a comparative pharmacokinetic study of fifteen components in rat plasma after oral administration of the NMT formula or single herb extracts to normal and stroke-afflicted rats. A principal component analysis (PCA) was utilized to evaluate the differences in the pharmacokinetic behavior (time-course) of the absorbed components of NMT, and the absorbed components were assigned to 3 separate clusters. A comparison of the body dynamics of each group indicated that cluster B (ginsenoside Rg1, ginsenoside Rb1, ginsenoside Rb3, ginsenoside Rc) might be the most important constituents controlling the pharmacological effects of NMT. The comparative pharmacokinetic study showed that the different groups had different pharmacokinetic characteristics. The pharmacokinetics-based UHPLC Quadrupole-Orbitrap-MS using a full-scan mode combined

  6. Genome Analysis of the Biotechnologically Relevant Acidophilic Iron Oxidising Strain JA12 Indicates Phylogenetic and Metabolic Diversity within the Novel Genus “Ferrovum”

    PubMed Central

    Ullrich, Sophie R.; Poehlein, Anja; Tischler, Judith S.; González, Carolina; Ossandon, Francisco J.; Daniel, Rolf; Holmes, David S.; Schlömann, Michael; Mühling, Martin

    2016-01-01

    Background Members of the genus “Ferrovum” are ubiquitously distributed in acid mine drainage (AMD) waters which are characterised by their high metal and sulfate loads. So far isolation and microbiological characterisation have only been successful for the designated type strain “Ferrovum myxofaciens” P3G. Thus, knowledge about physiological characteristics and the phylogeny of the genus “Ferrovum” is extremely scarce. Objective In order to access the wider genetic pool of the genus “Ferrovum” we sequenced the genome of a “Ferrovum”-containing mixed culture and successfully assembled the almost complete genome sequence of the novel “Ferrovum” strain JA12. Phylogeny and Lifestyle The genome-based phylogenetic analysis indicates that strain JA12 and the type strain represent two distinct “Ferrovum” species. “Ferrovum” strain JA12 is characterised by an unusually small genome in comparison to the type strain and other iron oxidising bacteria. The prediction of nutrient assimilation pathways suggests that “Ferrovum” strain JA12 maintains a chemolithoautotrophic lifestyle utilising carbon dioxide and bicarbonate, ammonium and urea, sulfate, phosphate and ferrous iron as carbon, nitrogen, sulfur, phosphorous and energy sources, respectively. Unique Metabolic Features The potential utilisation of urea by “Ferrovum” strain JA12 is moreover remarkable since it may furthermore represent a strategy among extreme acidophiles to cope with the acidic environment. Unlike other acidophilic chemolithoautotrophs “Ferrovum” strain JA12 exhibits a complete tricarboxylic acid cycle, a metabolic feature shared with the closer related neutrophilic iron oxidisers among the Betaproteobacteria including Sideroxydans lithotrophicus and Thiobacillus denitrificans. Furthermore, the absence of characteristic redox proteins involved in iron oxidation in the well-studied acidophiles Acidithiobacillus ferrooxidans (rusticyanin) and Acidithiobacillus

  7. Gene-to-metabolite network for biosynthesis of lignans in MeJA-elicited Isatis indigotica hairy root cultures

    PubMed Central

    Chen, Ruibing; Li, Qing; Tan, Hexin; Chen, Junfeng; Xiao, Ying; Ma, Ruifang; Gao, Shouhong; Zerbe, Philipp; Chen, Wansheng; Zhang, Lei

    2015-01-01

    Root and leaf tissue of Isatis indigotica shows notable anti-viral efficacy, and are widely used as “Banlangen” and “Daqingye” in traditional Chinese medicine. The plants' pharmacological activity is attributed to phenylpropanoids, especially a group of lignan metabolites. However, the biosynthesis of lignans in I. indigotica remains opaque. This study describes the discovery and analysis of biosynthetic genes and AP2/ERF-type transcription factors involved in lignan biosynthesis in I. indigotica. MeJA treatment revealed differential expression of three genes involved in phenylpropanoid backbone biosynthesis (IiPAL, IiC4H, Ii4CL), five genes involved in lignan biosynthesis (IiCAD, IiC3H, IiCCR, IiDIR, and IiPLR), and 112 putative AP2/ERF transcription factors. In addition, four intermediates of lariciresinol biosynthesis were found to be induced. Based on these results, a canonical correlation analysis using Pearson's correlation coefficient was performed to construct gene-to-metabolite networks and identify putative key genes and rate-limiting reactions in lignan biosynthesis. Over-expression of IiC3H, identified as a key pathway gene, was used for metabolic engineering of I. indigotica hairy roots, and resulted in an increase in lariciresinol production. These findings illustrate the utility of canonical correlation analysis for the discovery and metabolic engineering of key metabolic genes in plants. PMID:26579184

  8. The JaCVAM international validation study on the in vivo comet assay: Selection of test chemicals.

    PubMed

    Morita, Takeshi; Uno, Yoshifumi; Honma, Masamitsu; Kojima, Hajime; Hayashi, Makoto; Tice, Raymond R; Corvi, Raffaella; Schechtman, Leonard

    2015-07-01

    The Japanese Center for the Validation of Alternative Methods (JaCVAM) sponsored an international prevalidation and validation study of the in vivo rat alkaline pH comet assay. The main objective of the study was to assess the sensitivity and specificity of the assay for correctly identifying genotoxic carcinogens, as compared with the traditional rat liver unscheduled DNA synthesis assay. Based on existing carcinogenicity and genotoxicity data and chemical class information, 90 chemicals were identified as primary candidates for use in the validation study. From these 90 chemicals, 46 secondary candidates and then 40 final chemicals were selected based on a sufficiency of carcinogenic and genotoxic data, differences in chemical class or genotoxic or carcinogenic mode of action (MOA), availability, price, and ease of handling. These 40 chemicals included 19 genotoxic carcinogens, 6 genotoxic non-carcinogens, 7 non-genotoxic carcinogens and 8 non-genotoxic non-carcinogens. "Genotoxicity" was defined as positive in the Ames mutagenicity test or in one of the standard in vivo genotoxicity tests (primarily the erythrocyte micronucleus assay). These chemicals covered various chemicals classes, MOAs, and genotoxicity profiles and were considered to be suitable for the purpose of the validation study. General principles of chemical selection for validation studies are discussed.

  9. Extending MAM5 Meta-Model and JaCalIV E Framework to Integrate Smart Devices from Real Environments

    PubMed Central

    2016-01-01

    This paper presents the extension of a meta-model (MAM5) and a framework based on the model (JaCalIVE) for developing intelligent virtual environments. The goal of this extension is to develop augmented mirror worlds that represent a real and virtual world coupled, so that the virtual world not only reflects the real one, but also complements it. A new component called a smart resource artifact, that enables modelling and developing devices to access the real physical world, and a human in the loop agent to place a human in the system have been included in the meta-model and framework. The proposed extension of MAM5 has been tested by simulating a light control system where agents can access both virtual and real sensor/actuators through the smart resources developed. The results show that the use of real environment interactive elements (smart resource artifacts) in agent-based simulations allows to minimize the error between simulated and real system. PMID:26926691

  10. Extending MAM5 Meta-Model and JaCalIV E Framework to Integrate Smart Devices from Real Environments.

    PubMed

    Rincon, J A; Poza-Lujan, Jose-Luis; Julian, V; Posadas-Yagüe, Juan-Luis; Carrascosa, C

    2016-01-01

    This paper presents the extension of a meta-model (MAM5) and a framework based on the model (JaCalIVE) for developing intelligent virtual environments. The goal of this extension is to develop augmented mirror worlds that represent a real and virtual world coupled, so that the virtual world not only reflects the real one, but also complements it. A new component called a smart resource artifact, that enables modelling and developing devices to access the real physical world, and a human in the loop agent to place a human in the system have been included in the meta-model and framework. The proposed extension of MAM5 has been tested by simulating a light control system where agents can access both virtual and real sensor/actuators through the smart resources developed. The results show that the use of real environment interactive elements (smart resource artifacts) in agent-based simulations allows to minimize the error between simulated and real system.

  11. The Combined Effects of Ethylene and MeJA on Metabolic Profiling of Phenolic Compounds in Catharanthus roseus Revealed by Metabolomics Analysis.

    PubMed

    Liu, Jia; Liu, Yang; Wang, Yu; Zhang, Zhong-Hua; Zu, Yuan-Gang; Efferth, Thomas; Tang, Zhong-Hua

    2016-01-01

    Phenolic compounds belong to a class of secondary metabolites and are implicated in a wide range of responsive mechanisms in plants triggered by both biotic and abiotic elicitors. In this study, we approached the combinational effects of ethylene and MeJA (methyl jasmonate) on phenolic compounds profiles and gene expressions in the medicinal plant Catharanthus roseus. In virtue of a widely non-targeted metabolomics method, we identified a total of 34 kinds of phenolic compounds in the leaves, composed by 7 C6C1-, 11 C6C3-, and 16 C6C3C6 compounds. In addition, 7 kinds of intermediates critical for the biosynthesis of phenolic compounds and alkaloids were identified and discussed with phenolic metabolism. The combinational actions of ethylene and MeJA effectively promoted the total phenolic compounds, especially the C6C1 compounds (such as salicylic acid, benzoic acid) and C6C3 ones (such as cinnamic acid, sinapic acid). In contrast, the C6C3C6 compounds displayed a notably inhibitory trend in this case. Subsequently, the gene-to-metabolite networks were drawn up by searching for correlations between the expression profiles of 5 gene tags and the accumulation profiles of 41 metabolite peaks. Generally, we provide an insight into the controlling mode of ethylene-MeJA combination on phenolic metabolism in C. roseus leaves.

  12. OsNPR1 negatively regulates herbivore-induced JA and ethylene signaling and plant resistance to a chewing herbivore in rice.

    PubMed

    Li, Ran; Afsheen, Sumera; Xin, Zhaojun; Han, Xiu; Lou, Yonggen

    2013-03-01

    NPR1 (a non-expressor of pathogenesis-related genes1) has been reported to play an important role in plant defense by regulating signaling pathways. However, little to nothing is known about its function in herbivore-induced defense in monocot plants. Here, using suppressive substrate hybridization, we identified a NPR1 gene from rice, OsNPR1, and found that its expression levels were upregulated in response to infestation by the rice striped stem borer (SSB) Chilo suppressalis and rice leaf folder (LF) Cnaphalocrocis medinalis, and to mechanical wounding and treatment with jasmonic acid (JA) and salicylic acid (SA). Moreover, mechanical wounding induced the expression of OsNPR1 quickly, whereas herbivore infestation induced the gene more slowly. The antisense expression of OsNPR1 (as-npr1), which reduced the expression of the gene by 50%, increased elicited levels of JA and ethylene (ET) as well as of expression of a lipoxygenase gene OsHI-LOX and an ACC synthase gene OsACS2. The enhanced JA and ET signaling in as-npr1 plants increased the levels of herbivore-induced trypsin proteinase inhibitors (TrypPIs) and volatiles, and reduced the performance of SSB. Our results suggest that OsNPR1 is an early responding gene in herbivore-induced defense and that plants can use it to activate a specific and appropriate defense response against invaders by modulating signaling pathways.

  13. The Combined Effects of Ethylene and MeJA on Metabolic Profiling of Phenolic Compounds in Catharanthus roseus Revealed by Metabolomics Analysis

    PubMed Central

    Liu, Jia; Liu, Yang; Wang, Yu; Zhang, Zhong-Hua; Zu, Yuan-Gang; Efferth, Thomas; Tang, Zhong-Hua

    2016-01-01

    Phenolic compounds belong to a class of secondary metabolites and are implicated in a wide range of responsive mechanisms in plants triggered by both biotic and abiotic elicitors. In this study, we approached the combinational effects of ethylene and MeJA (methyl jasmonate) on phenolic compounds profiles and gene expressions in the medicinal plant Catharanthus roseus. In virtue of a widely non-targeted metabolomics method, we identified a total of 34 kinds of phenolic compounds in the leaves, composed by 7 C6C1-, 11 C6C3-, and 16 C6C3C6 compounds. In addition, 7 kinds of intermediates critical for the biosynthesis of phenolic compounds and alkaloids were identified and discussed with phenolic metabolism. The combinational actions of ethylene and MeJA effectively promoted the total phenolic compounds, especially the C6C1 compounds (such as salicylic acid, benzoic acid) and C6C3 ones (such as cinnamic acid, sinapic acid). In contrast, the C6C3C6 compounds displayed a notably inhibitory trend in this case. Subsequently, the gene-to-metabolite networks were drawn up by searching for correlations between the expression profiles of 5 gene tags and the accumulation profiles of 41 metabolite peaks. Generally, we provide an insight into the controlling mode of ethylene-MeJA combination on phenolic metabolism in C. roseus leaves. PMID:27375495

  14. Combination comet/micronucleus assay validation performed by BioReliance under the JaCVAM initiative.

    PubMed

    Pant, Kamala; Krsmanovic, Ljubica; Bruce, Shannon Wilson; Kelley, Tawney; Arevalo, Mirna; Atta-Safoh, Samuel; Debelie, Fekadu; La Force, Michelle L Klug; Springer, Sandra; Sly, Jamie; Paranjpe, Madhav; Lawlor, Timothy; Aardema, Marilyn

    2015-07-01

    In the international validation study of the in vivo rat alkaline comet assay (comet assay), the Japanese Center for the Validation of Alternative Methods (JaCVAM) provided three coded chemicals to BioReliance, 1,3-dichloropropene, ethionamide and busulfan, to be tested in a combined in vivo comet/micronucleus assay. Induction of DNA damage (comet) in liver, stomach and jejunum (1,3-dichloropropene only) cells, and induction of MNPCEs in bone marrow, were examined in male Sprague-Dawley (Hsd:SD) rats following oral administration of the test chemical for three consecutive days. A dose range finding (DRF) test was performed with each chemical to determine the maximum tolerated dose (MTD). Based on the results of the DRF test; 1,3-dichloropropene was tested at 50, 100 and 200 mg/kg/day; ethionamide was tested at 125, 250 and 500 mg/kg/day, and busulfan was tested at 10, 20 and 40 mg/kg/day. The results indicated that 1,3-dichloropropene induced DNA damage only in liver cells at all three test article doses, while no effects were observed in the stomach and jejunum cells. Additionally, it did not increase MNPCEs in the bone marrow. 1,3-Dichloropropene was concluded to be negative in the MN assay but positive in the comet assay. Ethionamide did not induce DNA damage in liver. However, in stomach, statistically significant decreases (although still within historical range) in % tail DNA at all test article doses compared to the vehicle control were observed. There was no increase in MNPCEs in the bone marrow. Thus, ethionamide was concluded to be negative in the comet/MN combined assay. Busulfan did not induce DNA damage in any of the organs tested (liver and stomach) but it did induce a significant increase in MNPCEs in the bone marrow. Busulfan was concluded to be negative in the comet assay but positive in the MN assay.

  15. LD Consultant Information in Mainstreaming: Help or Hindrance?

    ERIC Educational Resources Information Center

    Safran, Stephen; Barcikowski, Robert S.

    1984-01-01

    After receiving varying combinations of positive and negative communications on reading achievement, classroom behavior, and learning style from a "resource teacher" on a 12-year-old boy identified as learning disabled, 129 classroom teachers completed the "Regular Educator Expectancy Scale-Revised." Results confirmed…

  16. Enhancing ADHD and LD Diagnostic Accuracy Using Career Instruments

    ERIC Educational Resources Information Center

    Dipeolu, Abiola; Hargrave, Stephanie; Storlie, Cassandra A.

    2015-01-01

    Individuals diagnosed with mental health disorders may have work-related difficulties that impact functioning in all life domains. With limited research on the integration of career and mental health counseling, authors used a discriminant function analysis to assess the predictability of accurately identifying diagnostic categories among 258…

  17. 7 CFR 1737.32 - Loan Design (LD).

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... growth, must be discussed. Reasons for growth projections which vary from historic trends must be... exchange serving a community over 5,000 population. The name of the community, the number of existing and... population which had a population of more than 5,000 at the time the facilities to serve the community...

  18. 7 CFR 1737.32 - Loan Design (LD).

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... growth, must be discussed. Reasons for growth projections which vary from historic trends must be... exchange serving a community over 5,000 population. The name of the community, the number of existing and... population which had a population of more than 5,000 at the time the facilities to serve the community...

  19. Intervention Considerations in Mathematics for the LD Adolescent.

    ERIC Educational Resources Information Center

    Pieper, Edward; Deshler, Donald D.

    1985-01-01

    Some of the major curriculum and instructional problems that precipitate failure among learning disabled adolescents are discussed. A variety of intervention strategies to increase success are then presented. (MNS)

  20. Albert Einstein and LD: an evaluation of the evidence.

    PubMed

    Thomas, M

    2000-01-01

    Historical figures suspected of having learning disabilities are often subjected to retrospective diagnoses. One such figure is Albert Einstein. Several organizations that promote the interests of individuals with learning disabilities claim that Einstein had a learning disability. A review of biographical sources, however, provides little or no evidence to support this claim. The claim derives its force not from evidence but from a powerful belief--that the greatest among us suffer from some impairment--and from an equally powerful desire to enhance the status of a marginalized group by including within it exceptional individuals.

  1. ERDC-CERL LD-870 Download Program: Programming Manual

    DTIC Science & Technology

    2007-05-01

    CheckBox2.Enabled = False 1768 modem2.modemMode = Modem.Mode.ListenCancel 1769 BW2.CancelAsync() 1770...End If 1771 1772 End If 1773 1774 End Sub 1775 1776 Private Sub CheckBox3_CheckedChanged(ByVal sender As System.Object...Write("S1,1" & vbCr) 1767 result = ReadLine(CChar(vbLf)) 1768 result = result.Trim() 1769 If result Like

  2. Albert Einstein and LD: An Evaluation of the Evidence.

    ERIC Educational Resources Information Center

    Thomas, Marlin

    2000-01-01

    This article refutes claims that Albert Einstein had a learning disability and argues the claim derives its force not from evidence but from belief that the greatest among us suffer from some impairment and from desire to enhance the status of a marginalized group by including exceptional individuals. (Contains references.) (Author/CR)

  3. 7 CFR 1737.32 - Loan Design (LD).

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    .... The source and amount of any nonloan funds to be used for this construction must be discussed. (ii... growth, must be discussed. Reasons for growth projections which vary from historic trends must be... special note shall be submitted. Usually a separate short-term note is prepared for loans to...

  4. About L.D. Landau — the Great Physicist

    NASA Astrophysics Data System (ADS)

    Ginzburg, V. L.

    If not all, then very much can be known from comparison. Every time, when we call somebody: "great person", "a man of genius" —we make a comparison with other people. Although each person is unrepeatable it is clear, that some people differ from the average very much. At the same time all words undergo depreciation to some extent; we can observe the inflation of the epithets. Besides making an appearance we tend to speak well of everybody. This was understood even by ancients - they used to say that about people, who had passed away one should speak "either well or nothing". Surely this is the reason, why reading numerous memoirs we have the impression, that there are so many distinguished people. Indeed there are not so many…

  5. Blue laser diode (LD) and light emitting diode (LED) applications

    NASA Astrophysics Data System (ADS)

    Bergh, Arpad A.

    2004-09-01

    The family of blue LEDs, edge emitting and surface emitting lasers, enable a number of applications. Blue lasers are used in digital applications such as optical storage in high density DVDs. The resolution of the spot size and hence the storage density is diffraction limited and is inversely proportional to the square of the wavelength of the laser. Other applications include printing, optical scanners, and high-resolution photo-lithography.As light emitters, blue LEDs are used for signaling and in direct view large area emissive displays. They are also making inroads into signage and LCD back-lighting, mobile platforms, and decorative accent lighting in curtains, furniture, etc.Blue LEDs produce white light either with phosphor wavelength converters or in combination with red and green LEDs. The full potential of LED light sources will require three devices to enable complete control over color and intensity.Sensing and medical/bio applications have a major impact on home security, on monitoring the environment, and on health care. New emerging diagnostic and therapeutic applications will improve the quality and reduce the cost of health care.

  6. Acute Oral Toxicity (LD(50)) of CHF1 in Rats.

    DTIC Science & Technology

    1982-04-01

    dimethylsiloxane polymer) Chemical Abstract Service Registry No.: None Molecular structure: linear polydimethylsiloxa,,. Molecular weight: about 25,000 g/moles pH...discomfort may result if rubbed into the eye). 3. Chemical Name: isopropanol Chemical Abstract Service Registry No.: 67-63-0 Molecular structure: CH 3CHOHCH 3...isopropyl alcohol. The formulation is a suspension that must be agitated to maintain continuity. 1. Chemical Name: N,N-diethyl-n-toluamide Chemical

  7. 7 CFR 1737.32 - Loan Design (LD).

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... growth, must be discussed. Reasons for growth projections which vary from historic trends must be... exchange serving a community over 5,000 population. The name of the community, the number of existing and... population which had a population of more than 5,000 at the time the facilities to serve the community...

  8. Mineralogical characterization of tailing dams: incidence of abandoned mining works on soil pollution (Linares, Jaén)

    NASA Astrophysics Data System (ADS)

    de la Torre, M. J.; Hidalgo, C.; Rey, J.; Martínez, J.

    2012-04-01

    The metallogenic district of Linares-La Carolina (Jaén, Spain) consists of dyke mineralizations mainly of galena, accompanied by blende, chalcopyrite and barite. Associated to these abandoned mines, relatively extensive areas occupied by spoil heaps and tailing impoundments exist and constitute potential sources of soil pollution by metals and semimetals. In order to analyze the pollution potential of these mining wastes, we have carried out a mineralogical and geochemical study of seven tailing dams and surrounding soils in the area. The mineralogy of the samples was studied by x-ray diffraction (XRD) and scanning electron microscope (SEM). In addition, the total metal content of samples was determined by inductively coupled plasma mass spectrometry (ICP-MS) analysis. Samples were taken from the first 30 cm of the waste piles and soil deposits and white efflorescences were also obtained from the surface of the tailings. In all analyzed heaps, high to very high total contents in Pb (1220-22890 mg/kg), Zn (150-51280 mg/kg), Mn (2658-4160 mg/kg), Ba (1026-19610 mg/kg) and Fe (19400-138000 mg/kg) were observed. The concentrations for these same elements in the studied soils range from 527-9900 mg/kg for Pb, 27-1700 mg/kg for Zn, 506-2464 mg/kg for Mn, 2832-4306 for Ba and 8642-29753 mg/kg for Fe, and these figures indicate a contamination of the soils, according to the guidelines established by the Spanish law. The XRD and SEM results indicate that the tailings are primarily constituted by gangue of the exploited mineralization: quartz, calcite, ankerite, feldspars and phyllosilicates. They are inherited, primary mineral phases. Galena, also primary, appears in low proportion, as well as lepidocrocite, melanterite and cerussite, being these three last secondary minerals and indicating a certain remobilization of metal cations, especially lead and iron. On the other hand, quartz and phyllosilicates predominate in the soils, in which, in addition, is identified a

  9. PP2C-like Promoter and Its Deletion Variants Are Induced by ABA but Not by MeJA and SA in Arabidopsis thaliana.

    PubMed

    Bhalothia, Purva; Sangwan, Chetna; Alok, Anshu; Mehrotra, Sandhya; Mehrotra, Rajesh

    2016-01-01

    Gene expression is mediated through interaction between cis regulatory elements and its cognate transcription factors. Cis regulatory elements are defined as non-coding DNA sequences that provide the binding sites for transcription factors and are clustered in the upstream region of genes. ACGT cis regulatory element is one of the important cis regulatory elements found to be involved in diverse biological processes like auxin response, salicylic acid (SA) response, UV light response, ABA response and jasmonic acid (JA) response. We identified through in silico analysis that the upstream region of protein phosphatase 2C (PP2C) gene has a distinct genetic architecture of ACGT elements. In the present study, the activation of the full length promoter and its deletion constructs like 900 base pair, 500 base pair, 400 base pair and NRM (Nathji Rajesh Mehrotra) were examined by stable transformation in Arabidopsis thaliana using β-glucuronidase as the reporter gene. Evaluation of deletion constructs of PP2C-like promoter was carried out in the presence of phytohormones like abscisic acid (ABA), SA and JA. Our result indicated that the full length and 900 base pair promoter-reporter constructs of PP2C-like promoter was induced in response to ABA but not to methyl jasmonate and SA.

  10. Pro Memoria. Professor Bolesław Jałowy (1906-1943): Mortui viventes obligant - the livings are obligated to the dead.

    PubMed

    Wincewicz, Andrzej

    2016-01-01

    Professor Bolesław Jałowy (1906-1943) was a chairman of Department of Histology and Embryology at Faculty of Medicine of King John Casimir University (Polish: Universytet Jana Kazimierza: UJK) in Lvov. He succeeded Professor Władysław Szymonowicz (1869-1939) who held this position for decades. As the most skillful followers of his tutor, Bolesław Jałowy was a great investigator of physiology of human tissue, embryogenesis, histological consequences of female sex hormones on blood clotting action as well as regeneration of nerves in addition to description of silver staining technique for reticulin fibers of skin. He was a hard working person with gentle attitude to such a subtle matter as microscopic structure of human body. However, he happened to live in brutal conditions of nationalistic struggles. His example shows how much a dedicated scientist could do in a very short time as his life was tragically ended with murdering him during World War Two. His story is a great lesson for generations of academic workers how to meet high moral standards with efficient and creative scientific work in evil and destructive, nationalistic climate that occurs usually in wartime.

  11. Investigation of sodium arsenite, thioacetamide, and diethanolamine in the alkaline comet assay: Part of the JaCVAM comet validation exercise.

    PubMed

    Beevers, Carol; Henderson, Debbie; Lillford, Lucinda

    2015-07-01

    As part of the Japanese Center for the Validation of Alternative Methods (JaCVAM)-initiative international validation study of the in vivo rat alkaline comet assay (comet assay), we examined sodium arsenite, thioacetamide, and diethanolamine. Using the JaCVAM approved study protocol version 14.2, each chemical was tested in male rats up to maximum tolerated dose levels and DNA damage in the liver and stomach was assessed approximately 3h after the final administration by gavage. Histopathology assessments of liver and stomach sections from the same animals were also examined for evidence of cytotoxicity or necrosis. No evidence of DNA damage was observed in the stomach of animals treated with sodium arsenite at 7.5, 15, or 30 mg/kg/day. However, equivocal findings were found in the liver, where increases in DNA migration were observed in two independent experiments, but not in all treated animals and not at the same dose levels. Thioacetamide caused an increase in DNA migration in the stomach of rats treated at 19, 38, and 75 mg/kg/day, but not in the liver, despite evidence of marked hepatotoxicity following histopathology assessments. No evidence of DNA damage was observed in the stomach or liver of animals treated with diethanolamine at 175, 350, or 700 mg/kg/day.

  12. Ka Whanau mai te Reo: Honouring Whanau, Upholding Reo Maori

    ERIC Educational Resources Information Center

    Bright, Nicola; Barnes, Alex; Hutchings, Jessica

    2013-01-01

    This is the first report from a 3-year (2012-2015) kaupapa Maori research project that investigates how best to support the continuity of reo Maori development of whanau as they transition between kohanga reo, kura kaupapa primary, wharekura, secondary and beyond. It aims to provide useful information about the reo Maori education options that are…

  13. SAR For REDD+ in the Mai Ndombe District (DRC)

    NASA Astrophysics Data System (ADS)

    Haarpaintner, Jorg

    2016-08-01

    The overall goal of the project "SAR for REDD" is to provide cloud-penetrating satellite synthetic aperture radar (SAR) pre-processing and analysing capabilities and tools to support operational tropical forest monitoring in REDD countries and primarily in Africa. The project's end-user is the Observatoir Satellitale des Forêts d'Afrique Centrale (OSFAC).This paper presents an overall summary of the project and shows first results of the satellite products, that will be delivered to the user in addition to software tools to enhance the user's own technical capacity.The products shown here are SAR mosaics and derived forest-land cover maps based on C-band Sentinel-1A data for 2015, ALOS-PALSAR data for the period 2007-2010 and ALOS-2 PALSAR-2 for 2015. In addition, a forest cover change map from 2007 to 2010 based on ALOS PALSAR has been produced and is compared to results from the Global Forest Cover project [1].

  14. Hypogean pseudoscorpions (Arachnida) from Jaén province (Andalusia, Spain), with descriptions of four new species and a new synonymy.

    PubMed

    Zaragoza, Juan A; Pérez, Toni

    2013-01-01

    Four new hypogean species are described from the Jaén province (southern Spain): Chthonius (Ephippiochthonius) espa- nyoli sp. nov., C. (E.) giennensis sp. nov., C. (E.) villacarrillo sp. nov. and Neobisium (Ommatoblothrus) perezruizi sp. nov. New records are given for the species Chthonius (E.) cazorlensis, C. (E.) perezi, C. (E.) tetrachelatus, Neobisium (O.) perezi, Microcreagrella caeca caeca and Allochernes masi. Chthonius (E.) verai and C. (E.) minutus are removed from the list of the Andalusian fauna. A new synonymy is proposed: Neobisium (O.) gev Carabajal Márquez, García Carrillo & Rodríguez Fernández, 2011, is a junior subjective synonym of N. (O.) perezi Carabajal Márquez, García Carrillo & Rodríguez Fernández, 2011.

  15. Monitoring of selected priority and emerging contaminants in the Guadalquivir River and other related surface waters in the province of Jaén, South East Spain.

    PubMed

    Robles-Molina, José; Gilbert-López, Bienvenida; García-Reyes, Juan F; Molina-Díaz, Antonio

    2014-05-01

    The province of Jaén counts with four natural parks, numerous rivers, reservoirs and wetlands; moreover, it is probably the region with higher olive oil production in the world, which makes this zone a proper target to be studied based on the European Water Framework Directive 2000/60/CE. The aim of this survey is to monitor a total number of 373 compounds belonging to different families (pesticides, PAHs, nitrosamines, drugs of abuse, pharmaceuticals and life-style compounds) in surface waters located at different points of the province of Jaén. Among these compounds some priority organic substances (regulated by the EU Directive 2008/105/EC) and pollutants of emerging concern (not regulated yet) can be found. A liquid chromatography electrospray time-of-flight mass spectrometry (LC-TOFMS) method covering 340 compounds was developed and applied, together with a gas chromatography triple-quadrupole mass spectrometry (GC-MS/MS) method which enabled the analysis of 63 organic contaminants (30 of these compounds are analyzed by LC-TOFMS as well). From April 2009 to November 2010 a total of 83 surface water samples were collected (rivers, reservoirs and wetlands). In this period numerous organic contaminants were detected, most of them at the ng L(-1) level. The most frequently priority substances found were chlorpyrifos ethyl, diuron and hexachlorobenzene. Within the other groups, the most frequently detected compounds were: terbuthylazine, oxyfluorfen, desethyl terbuthylazine, diphenylamine (pesticide family); fluorene, phenanthrene, pyrene (PAHs group), codeine, paracetamol (pharmaceuticals compounds) and caffeine, nicotine (life-style compounds). As is could be expected, the total concentration of emerging contaminants is distinctly larger than that of priority pollutants, highlighting the importance of continuing with the study of their presence, fate and effects in aquatic environments. However, concentration levels (at the ng per liter level) are low in

  16. Molecular characterisation of extended-spectrum β-lactamase- and plasmid AmpC-producing Escherichia coli strains isolated from broilers in Béjaïa, Algeria.

    PubMed

    Belmahdi, Mohamed; Bakour, Sofiane; Al Bayssari, Charbel; Touati, Abdelaziz; Rolain, Jean-Marc

    2016-09-01

    This study aimed to characterise the molecular support of antibiotic resistance in expanded-spectrum cephalosporin (ESC)-resistant Escherichia coli isolates recovered from healthy broilers in Béjaïa, northeast Algeria. A total of 61 intestinal swabs from slaughtered broilers from four regions in Béjaïa locality, Algeria, were collected between February and April 2014, from which 20 ESC-resistant E. coli strains were isolated. Escherichia coli isolates were identified by classical biochemical and MALDI-TOF methods. Antibiotic susceptibility testing was performed using disk diffusion and Etest methods. Screening for β-lactamases, aminoglycoside-modifying enzyme (AME)-encoding genes and qnr determinants was performed by PCR and sequencing. Clonal relatedness was determined using molecular typing by multilocus sequence typing (MLST). Antibiotic susceptibility testing revealed that the isolates showed high rates of resistance (>90%) to amoxicillin, amoxicillin/clavulanic acid, piperacillin/tazobactam, aztreonam, ceftazidime, streptomycin, tobramycin, nalidixic acid and ciprofloxacin. Low rates of resistance were observed for kanamycin (35%), amikacin (30%), cefoxitin (20%) and cefotaxime (15%). Molecular characterisation revealed that all of the isolates expressed the blaTEM-1 gene. Fourteen of them harboured the blaSHV-12 gene, two harboured the blaCTX-M-1 gene and four isolates harboured blaCMY-2. Screening for AME-encoding genes demonstrated that all isolates contained the aadA gene. In addition, qnrA was detected as the quinolone resistance determinant in 13 isolates. MLST revealed four known sequence types (STs), including ST744, ST38, ST1011 and ST2179, as well as one new sequence type (ST5086). Here we report the first study describing the clonal diversity of extended-spectrum β-lactamase (ESBL)- and plasmid AmpC-producing E. coli isolated from healthy broilers in Algeria.

  17. Re-analysis results using medians of the data from the JaCVAM-organized international validation study of the in vivo rat alkaline comet assay.

    PubMed

    Uno, Yoshifumi; Omori, Takashi

    2015-07-01

    The data from the JaCVAM-organized international validation study of the in vivo rat alkaline comet assay were reported and analyzed statistically using the simple means of % tail DNA. However, OECD test guideline TG 489 recommends use of the median for data analysis due to the hierarchical nature of the data. Comparison between the simple mean approach and the median based approach for positive/negative/equivocal chemical calls was conducted using the % tail DNA data for the 40 chemicals tested in the JaCVAM-organized international validation study of the in vivo rat alkaline comet assay, using liver and stomach as target organs. In the liver, two genotoxic chemicals, o-anisidine and 9-aminoacridine hydrochloride monohydrate, were positive using the median based approach but negative using the simple mean approach, and two genotoxic chemicals, 2-acetylaminofluorene and busulfan were equivocal using the median based approach but negative using the simple mean approach. In contrast, cadmium chloride (genotoxic carcinogen) was equivocal in both organs using the median based approach, while positive and equivocal in liver and stomach, respectively, using the simple mean approach. Two data sets of sodium arsenite showed equivocal and negative results for liver using the median based approach, although both data sets were equivocal using the simple mean approach. Overall, there are no large differences in terms of the genotoxic call between both approaches. However, the median based approach recommended in OECD TG 489 has an advantage toward higher precision within the groups treated with a test chemical, whereas the approach might show the lower values for the effect.

  18. Variability of phenotype, anthocyanin indexes, and flavonoids in accessions from a close relative of soybean, Neontonia wightii (Wright & Arn. J.A. Lackey) in the U.S. germplasm collection for potential use as a health forage

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A close relative of soybean, Neonotonia wightii (Wright & Arn. J.A. Lackey), is used as a ruminant feed and restores soil productivity in Brazil and Zimbabwe, respectively. Neonotonia wightii accessions were grown in a greenhouse at Griffin, Georgia, characterized for various phenotypic traits, and...

  19. Simulated herbivory in chickpea causes rapid changes in defense pathways and hormonal transcription networks of JA/ethylene/GA/auxin within minutes of wounding

    PubMed Central

    Pandey, Saurabh Prakash; Srivastava, Shruti; Goel, Ridhi; Lakhwani, Deepika; Singh, Priya; Asif, Mehar Hasan; Sane, Aniruddha P.

    2017-01-01

    Chickpea (C. arietinum L.) is an important pulse crop in Asian and African countries that suffers significant yield losses due to attacks by insects like H. armigera. To obtain insights into early responses of chickpea to insect attack, a transcriptomic analysis of chickpea leaves just 20 minutes after simulated herbivory was performed, using oral secretions of H. armigera coupled with mechanical wounding. Expression profiles revealed differential regulation of 8.4% of the total leaf transcriptome with 1334 genes up-regulated and 501 down-regulated upon wounding at log2-fold change (|FC| ≤ −1 and ≥1) and FDR value ≤ 0.05. In silico analysis showed the activation of defenses through up-regulation of genes of the phenylpropanoid pathway, pathogenesis, oxidases and CYTP450 besides differential regulation of kinases, phosphatases and transcription factors of the WRKY, MYB, ERFs, bZIP families. A substantial change in the regulation of hormonal networks was observed with up-regulation of JA and ethylene pathways and suppression of growth associated hormone pathways like GA and auxin within 20 minutes of wounding. Secondary qPCR comparison of selected genes showed that oral secretions often increased differential expression relative to mechanical damage alone. The studies provide new insights into early wound responses in chickpea. PMID:28300183

  20. LOW-TEMPERATURE ION TRAP STUDIES OF N{sup +}({sup 3} P{sub ja} ) + H{sub 2}(j) {yields} NH{sup +} + H

    SciTech Connect

    Zymak, I.; Hejduk, M.; Mulin, D.; Plasil, R.; Glosik, J.; Gerlich, D.

    2013-05-01

    Using a low-temperature 22-pole ion trap apparatus, detailed measurements for the title reaction have been performed between 10 K and 100 K in order to get some state specific information about this fundamental hydrogen abstraction process. The relative population of the two lowest H{sub 2} rotational states, j = 0 and 1, has been varied systematically. NH{sup +} formation is nearly thermo-neutral; however, to date, the energetics are not known with the accuracy required for low-temperature astrochemistry. Additional complications arise from the fact that, so far, there is no reliable theoretical or experimental information on how the reactivity of the N{sup +} ion depends on its fine-structure (FS) state {sup 3} P{sub ja} . Since in the present trapping experiment, thermalization of the initially hot FS population competes with hydrogen abstraction, the evaluation of the decay of N{sup +} ions over long storage times and at various He and H{sub 2} gas densities provides information on these processes. First assuming strict adiabatic behavior, a set of state specific rate coefficients is derived from the measured thermal rate coefficients. In addition, by recording the disappearance of the N{sup +} ions over several orders of magnitude, information on nonadiabatic transitions is extracted including FS-changing collisions.

  1. Hydrogeological research on intensively exploited deep aquifers in the `Loma de Úbeda' area (Jaén, southern Spain)

    NASA Astrophysics Data System (ADS)

    González-Ramón, Antonio; Rodríguez-Arévalo, Javier; Martos-Rosillo, Sergio; Gollonet, Javier

    2013-06-01

    The intensive use of groundwater for irrigation in the area of Úbeda (`Loma de Úbeda', Jaén, southern Spain) has transformed an area of traditionally rain-fed dry farmland into fields with some of the highest olive oil productivity in the world. Early hydrogeological research studies, initiated just after the beginning of the groundwater exploitation, revealed that the water was collected from three different overlapping aquifers occupying an area of over 1,100 km2, with the lower aquifers located at depths from 300 to over 700 m in an area of 440 km2. Multidisciplinary research, based on geological characterization, and piezometric, hydrochemical and isotopic data, has led to a conceptual model of functioning in this complex hydrogeological system. The proposed model allows for the identification of the recharge areas, and the discharge, which is at present mainly associated with the groundwater pumping. Areas of mixing of waters from the different aquifers and the main hydrogeochemical processes affecting groundwater quality are described.

  2. Simulated herbivory in chickpea causes rapid changes in defense pathways and hormonal transcription networks of JA/ethylene/GA/auxin within minutes of wounding.

    PubMed

    Pandey, Saurabh Prakash; Srivastava, Shruti; Goel, Ridhi; Lakhwani, Deepika; Singh, Priya; Asif, Mehar Hasan; Sane, Aniruddha P

    2017-03-16

    Chickpea (C. arietinum L.) is an important pulse crop in Asian and African countries that suffers significant yield losses due to attacks by insects like H. armigera. To obtain insights into early responses of chickpea to insect attack, a transcriptomic analysis of chickpea leaves just 20 minutes after simulated herbivory was performed, using oral secretions of H. armigera coupled with mechanical wounding. Expression profiles revealed differential regulation of 8.4% of the total leaf transcriptome with 1334 genes up-regulated and 501 down-regulated upon wounding at log2-fold change (|FC| ≤ -1 and ≥1) and FDR value ≤ 0.05. In silico analysis showed the activation of defenses through up-regulation of genes of the phenylpropanoid pathway, pathogenesis, oxidases and CYTP450 besides differential regulation of kinases, phosphatases and transcription factors of the WRKY, MYB, ERFs, bZIP families. A substantial change in the regulation of hormonal networks was observed with up-regulation of JA and ethylene pathways and suppression of growth associated hormone pathways like GA and auxin within 20 minutes of wounding. Secondary qPCR comparison of selected genes showed that oral secretions often increased differential expression relative to mechanical damage alone. The studies provide new insights into early wound responses in chickpea.

  3. Physical and metabolic interactions of Pseudomonas sp. strain JA5-B45 and Rhodococcus sp. strain F9-D79 during growth on crude oil and effect of a chemical surfactant on them.

    PubMed

    Van Hamme, J D; Ward, O P

    2001-10-01

    Methods to enhance crude oil biodegradation by mixed bacterial cultures, for example, (bio)surfactant addition, are complicated by the diversity of microbial populations within a given culture. The physical and metabolic interactions between Rhodococcus sp. strain F9-D79 and Pseudomonas sp. strain JA5-B45 were examined during growth on Bow River crude oil. The effects of a nonionic chemical surfactant, Igepal CO-630 (nonylphenol ethoxylate), also were evaluated. Strain F9-D79 grew attached to the oil-water interface and produced a mycolic acid-containing capsule. Crude oil emulsification and surface activity were associated with the cellular fraction. Strain JA5-B45 grew in the aqueous phase and was unable to emulsify oil, but cell-free supernatants mediated kerosene-water emulsion formation. In coculture, stable emulsions were formed and strain JA5-B45 had an affinity for the capsule produced by strain F9-D79. Igepal CO-630 inhibited F9-D79 cells from adhering to the interface, and cells grew dispersed in the aqueous phase as 0.5-microm cocci rather than 2.5-microm rods. The surfactant increased total petroleum hydrocarbon removal by strain JA5-B45 from 4 to 22% and included both saturated compounds and aromatics. In coculture, TPH removal increased from 13 to 40% following surfactant addition. The culture pH normally increased from 7.0 to between 7.5 and 8.5, although addition of Igepal CO-630 to F9-D79 cultures resulted in a drop to pH 5.5. We suggest a dual role for the nonylphenol ethoxylate surfactant in the coculture: (i) to improve hydrocarbon uptake by strain JA5-B45 through emulsification and (ii) to prevent strain F9-D79 from adhering to the oil-water interface, indirectly increasing hydrocarbon availability. These varied effects on hydrocarbon biodegradation could explain some of the known diversity of surfactant effects.

  4. Physical and Metabolic Interactions of Pseudomonas sp. Strain JA5-B45 and Rhodococcus sp. Strain F9-D79 during Growth on Crude Oil and Effect of a Chemical Surfactant on Them

    PubMed Central

    Van Hamme, Jonathan D.; Ward, Owen P.

    2001-01-01

    Methods to enhance crude oil biodegradation by mixed bacterial cultures, for example, (bio)surfactant addition, are complicated by the diversity of microbial populations within a given culture. The physical and metabolic interactions between Rhodococcus sp. strain F9-D79 and Pseudomonas sp. strain JA5-B45 were examined during growth on Bow River crude oil. The effects of a nonionic chemical surfactant, Igepal CO-630 (nonylphenol ethoxylate), also were evaluated. Strain F9-D79 grew attached to the oil-water interface and produced a mycolic acid-containing capsule. Crude oil emulsification and surface activity were associated with the cellular fraction. Strain JA5-B45 grew in the aqueous phase and was unable to emulsify oil, but cell-free supernatants mediated kerosene-water emulsion formation. In coculture, stable emulsions were formed and strain JA5-B45 had an affinity for the capsule produced by strain F9-D79. Igepal CO-630 inhibited F9-D79 cells from adhering to the interface, and cells grew dispersed in the aqueous phase as 0.5-μm cocci rather than 2.5-μm rods. The surfactant increased total petroleum hydrocarbon removal by strain JA5-B45 from 4 to 22% and included both saturated compounds and aromatics. In coculture, TPH removal increased from 13 to 40% following surfactant addition. The culture pH normally increased from 7.0 to between 7.5 and 8.5, although addition of Igepal CO-630 to F9-D79 cultures resulted in a drop to pH 5.5. We suggest a dual role for the nonylphenol ethoxylate surfactant in the coculture: (i) to improve hydrocarbon uptake by strain JA5-B45 through emulsification and (ii) to prevent strain F9-D79 from adhering to the oil-water interface, indirectly increasing hydrocarbon availability. These varied effects on hydrocarbon biodegradation could explain some of the known diversity of surfactant effects. PMID:11571196

  5. JaCVAM-organized international validation study of the in vivo rodent alkaline comet assay for detection of genotoxic carcinogens: II. Summary of definitive validation study results.

    PubMed

    Uno, Yoshifumi; Kojima, Hajime; Omori, Takashi; Corvi, Raffaella; Honma, Masamistu; Schechtman, Leonard M; Tice, Raymond R; Beevers, Carol; De Boeck, Marlies; Burlinson, Brian; Hobbs, Cheryl A; Kitamoto, Sachiko; Kraynak, Andrew R; McNamee, James; Nakagawa, Yuzuki; Pant, Kamala; Plappert-Helbig, Ulla; Priestley, Catherine; Takasawa, Hironao; Wada, Kunio; Wirnitzer, Uta; Asano, Norihide; Escobar, Patricia A; Lovell, David; Morita, Takeshi; Nakajima, Madoka; Ohno, Yasuo; Hayashi, Makoto

    2015-07-01

    The in vivo rodent alkaline comet assay (comet assay) is used internationally to investigate the in vivo genotoxic potential of test chemicals. This assay, however, has not previously been formally validated. The Japanese Center for the Validation of Alternative Methods (JaCVAM), with the cooperation of the U.S. NTP Interagency Center for the Evaluation of Alternative Toxicological Methods (NICEATM)/the Interagency Coordinating Committee on the Validation of Alternative Methods (ICCVAM), the European Centre for the Validation of Alternative Methods (ECVAM), and the Japanese Environmental Mutagen Society/Mammalian Mutagenesis Study Group (JEMS/MMS), organized an international validation study to evaluate the reliability and relevance of the assay for identifying genotoxic carcinogens, using liver and stomach as target organs. The ultimate goal of this exercise was to establish an Organisation for Economic Co-operation and Development (OECD) test guideline. The study protocol was optimized in the pre-validation studies, and then the definitive (4th phase) validation study was conducted in two steps. In the 1st step, assay reproducibility was confirmed among laboratories using four coded reference chemicals and the positive control ethyl methanesulfonate. In the 2nd step, the predictive capability was investigated using 40 coded chemicals with known genotoxic and carcinogenic activity (i.e., genotoxic carcinogens, genotoxic non-carcinogens, non-genotoxic carcinogens, and non-genotoxic non-carcinogens). Based on the results obtained, the in vivo comet assay is concluded to be highly capable of identifying genotoxic chemicals and therefore can serve as a reliable predictor of rodent carcinogenicity.

  6. Comparative genomic analysis of single-molecule sequencing and hybrid approaches for finishing the Clostridium autoethanogenum JA1-1 strain DSM 10061 genome

    SciTech Connect

    Brown, Steven D; Nagaraju, Shilpa; Utturkar, Sagar M; De Tissera, Sashini; Segovia, Simón; Mitchell, Wayne; Land, Miriam L; Dassanayake, Asela; Köpke, Michael

    2014-01-01

    Background Clostridium autoethanogenum strain JA1-1 (DSM 10061) is an acetogen capable of fermenting CO, CO2 and H2 (e.g. from syngas or waste gases) into biofuel ethanol and commodity chemicals such as 2,3-butanediol. A draft genome sequence consisting of 100 contigs has been published. Results A closed, high-quality genome sequence for C. autoethanogenum DSM10061 was generated using only the latest single-molecule DNA sequencing technology and without the need for manual finishing. It is assigned to the most complex genome classification based upon genome features such as repeats, prophage, nine copies of the rRNA gene operons. It has a low G + C content of 31.1%. Illumina, 454, Illumina/454 hybrid assemblies were generated and then compared to the draft and PacBio assemblies using summary statistics, CGAL, QUAST and REAPR bioinformatics tools and comparative genomic approaches. Assemblies based upon shorter read DNA technologies were confounded by the large number repeats and their size, which in the case of the rRNA gene operons were ~5 kb. CRISPR (Clustered Regularly Interspaced Short Paloindromic Repeats) systems among biotechnologically relevant Clostridia were classified and related to plasmid content and prophages. Potential associations between plasmid content and CRISPR systems may have implications for historical industrial scale Acetone-Butanol-Ethanol (ABE) fermentation failures and future large scale bacterial fermentations. While C. autoethanogenum contains an active CRISPR system, no such system is present in the closely related Clostridium ljungdahlii DSM 13528. A common prophage inserted into the Arg-tRNA shared between the strains suggests a common ancestor. However, C. ljungdahlii contains several additional putative prophages and it has more than double the amount of prophage DNA compared to C. autoethanogenum. Other differences include important metabolic genes for central metabolism (as an additional hydrogenase and the absence of a

  7. Citrus leprosis virus C Infection Results in Hypersensitive-Like Response, Suppression of the JA/ET Plant Defense Pathway and Promotion of the Colonization of Its Mite Vector

    PubMed Central

    Arena, Gabriella D.; Ramos-González, Pedro L.; Nunes, Maria A.; Ribeiro-Alves, Marcelo; Camargo, Luis E. A.; Kitajima, Elliot W.; Machado, Marcos A.; Freitas-Astúa, Juliana

    2016-01-01

    Leprosis is a serious disease of citrus caused by Citrus leprosis virus C (CiLV-C, genus Cilevirus) whose transmission is mediated by false spider mites of the genus Brevipalpus. CiLV-C infection does not systemically spread in any of its known host plants, thus remaining restricted to local lesions around the feeding sites of viruliferous mites. To get insight into this unusual pathosystem, we evaluated the expression profiles of genes involved in defense mechanisms of Arabidopsis thaliana and Citrus sinensis upon infestation with non-viruliferous and viruliferous mites by using reverse-transcription qPCR. These results were analyzed together with the production of reactive oxygen species (ROS) and the appearance of dead cells as assessed by histochemical assays. After interaction with non-viruliferous mites, plants locally accumulated ROS and triggered the salicylic acid (SA) and jasmonate/ethylene (JA/ET) pathways. ERF branch of the JA/ET pathways was highly activated. In contrast, JA pathway genes were markedly suppressed upon the CiLV-C infection mediated by viruliferous mites. Viral infection also intensified the ROS burst and cell death, and enhanced the expression of genes involved in the RNA silencing mechanism and SA pathway. After 13 days of infestation of two sets of Arabidopsis plants with non-viruliferous and viruliferous mites, the number of mites in the CiLV-C infected Arabidopsis plants was significantly higher than in those infested with the non-viruliferous ones. Oviposition of the viruliferous mites occurred preferentially in the CiLV-C infected leaves. Based on these results, we postulated the first model of plant/Brevipalpus mite/cilevirus interaction in which cells surrounding the feeding sites of viruliferous mites typify the outcome of a hypersensitive-like response, whereas viral infection induces changes in the behavior of its vector. PMID:27933078

  8. Perceived Close Relationships with Parents, Teachers, and Peers: Predictors of Social, Emotional, and Behavioral Features in Adolescents With LD or Comorbid LD and ADHD

    ERIC Educational Resources Information Center

    Al-Yagon, Michal

    2016-01-01

    This study examined the role of adolescents' perceived close relationships with significant others (attachment relationships with mothers/fathers, appraisal of homeroom teacher as secure base, and quality of peer friendship) in explaining differences in their socioemotional and behavioral functioning (peer-network/peer-dyadic loneliness,…

  9. Buckwheat (Fagopyrum esculentum M.) sprout treated with methyl jasmonate (MeJA) improved anti-adipogenic activity associated with the oxidative stress system in 3T3-L1 adipocytes.

    PubMed

    Lee, Young-Jun; Kim, Kui-Jin; Park, Kee-Jai; Yoon, Bo-Ra; Lim, Jeong-Ho; Lee, Ok-Hwan

    2013-01-11

    Buckwheat sprouts contain various bioactive compounds including rutin which have a number of biological activities. We have previously shown that buckwheat sprouts (TBWE) treated with methyl jasmonate (MeJA) significantly increased the amount of phenolics and the antioxidant activity. The aim of this study was to demonstrate the effect of TBWE on anti-adipogenesis and pro-oxidant enzyme in 3T3-L1 adipocytes. We also evaluated the anti-oxidative activity of TBWE in adipocytes by using the nitroblue tetrazolium assay. Our data showed that TBWE markedly inhibited adipocyte differentiation and ROS production in 3T3-L1 cells compared with control groups. Moreover, TBWE has strongly shown the inhibition of adipogenic transcription factor as well as pro-oxidant enzymes. Together, we demonstrate that the MeJA treatment significantly increased the amount of phenolic compound, resulting in the suppression of adipogenesis and ROS production in the 3T3-L1 cells. These findings indicate that TBWE has the potential for anti-adipogenesis activity with anti-oxidative properties.

  10. Induced Systemic Resistance against Botrytis cinerea by Bacillus cereus AR156 through a JA/ET- and NPR1-Dependent Signaling Pathway and Activates PAMP-Triggered Immunity in Arabidopsis

    PubMed Central

    Nie, Pingping; Li, Xia; Wang, Shune; Guo, Jianhua; Zhao, Hongwei; Niu, Dongdong

    2017-01-01

    Induced resistance response is a potent and cost effective plant defense against pathogen attack. The effectiveness and underlying mechanisms of the suppressive ability by Bacillus cereus AR156 to Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) in Arabidopsis has been investigated previously; however, the strength of induced systemic resistance (ISR) activity against Botrytis cinerea remains unknown. Here, we show that root-drench application of AR156 significantly reduces disease incidence through activation of ISR. This protection is accompanied with multilayered ISR defense response activated via enhanced accumulation of PR1 protein expression in a timely manner, hydrogen peroxide accumulation and callose deposition, which is significantly more intense in plants with both AR156 pretreatment and B. cinerea inoculation than that in plants with pathogen inoculation only. Moreover, AR156 can trigger ISR in sid2-2 and NahG mutants, but not in jar1, ein2 and npr1 mutant plants. Our results indicate that AR156-induced ISR depends on JA/ET-signaling pathway and NPR1, but not SA. Also, AR156-treated plants are able to rapidly activate MAPK signaling and FRK1/WRKY53 gene expression, both of which are involved in pathogen associated molecular pattern (PAMP)-triggered immunity (PTI). The results indicate that AR156 can induce ISR by the JA/ET-signaling pathways in an NPR1-dependent manner and involves multiple PTI components. PMID:28293243

  11. Induced Systemic Resistance against Botrytis cinerea by Bacillus cereus AR156 through a JA/ET- and NPR1-Dependent Signaling Pathway and Activates PAMP-Triggered Immunity in Arabidopsis.

    PubMed

    Nie, Pingping; Li, Xia; Wang, Shune; Guo, Jianhua; Zhao, Hongwei; Niu, Dongdong

    2017-01-01

    Induced resistance response is a potent and cost effective plant defense against pathogen attack. The effectiveness and underlying mechanisms of the suppressive ability by Bacillus cereus AR156 to Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) in Arabidopsis has been investigated previously; however, the strength of induced systemic resistance (ISR) activity against Botrytis cinerea remains unknown. Here, we show that root-drench application of AR156 significantly reduces disease incidence through activation of ISR. This protection is accompanied with multilayered ISR defense response activated via enhanced accumulation of PR1 protein expression in a timely manner, hydrogen peroxide accumulation and callose deposition, which is significantly more intense in plants with both AR156 pretreatment and B. cinerea inoculation than that in plants with pathogen inoculation only. Moreover, AR156 can trigger ISR in sid2-2 and NahG mutants, but not in jar1, ein2 and npr1 mutant plants. Our results indicate that AR156-induced ISR depends on JA/ET-signaling pathway and NPR1, but not SA. Also, AR156-treated plants are able to rapidly activate MAPK signaling and FRK1/WRKY53 gene expression, both of which are involved in pathogen associated molecular pattern (PAMP)-triggered immunity (PTI). The results indicate that AR156 can induce ISR by the JA/ET-signaling pathways in an NPR1-dependent manner and involves multiple PTI components.

  12. Four new species of Pomphopsilla Jałoszyński and a new record of Cephennodes glabella Castellini in the Democratic Republic of the Congo
    (Coleoptera: Staphylinidae: Scydmaeninae).

    PubMed

    Jałoszyński, Paweł

    2016-04-07

    Four new species of the genus Pomphopsilla Jałoszyński are described, all based on specimens collected in the north-eastern part of DR Congo: P. pygmaea sp. n., P. pseudosoror sp. n., P. similis sp. n., and P. gumovskyi sp. n. Previously Pomphopsilla, represented by two species, was known to occur in Kenya; the only genera of Cephenniini recorded from DR Congo were Cephennodes Reitter and Cephennomicrus Reitter. Cephennodes (s. str.) glabella Castellini, so far known only from the type series, is recorded from the same region of Congo, based on numerous specimens collected by yellow pan traps. Because figures in the original description of this species were relatively schematic, the aedeagus and modifications of the head in the male are illustrated.

  13. Evaluation of methyl methanesulfonate, 2,6-diaminotoluene and 5-fluorouracil: Part of the Japanese center for the validation of alternative methods (JaCVAM) international validation study of the in vivo rat alkaline comet assay.

    PubMed

    Plappert-Helbig, Ulla; Junker-Walker, Ursula; Martus, Hans-Joerg

    2015-07-01

    As a part of the Japanese Center for the Validation of Alternative Methods (JaCVAM)-initiative international validation study of the in vivo rat alkaline comet assay (comet assay), we examined methyl methanesulfonate, 2,6-diaminotoluene, and 5-fluorouracil under coded test conditions. Rats were treated orally with the maximum tolerated dose (MTD) and two additional descending doses of the respective compounds. In the MMS treated groups liver and stomach showed significantly elevated DNA damage at each dose level and a significant dose-response relationship. 2,6-diaminotoluene induced significantly elevated DNA damage in the liver at each dose and a statistically significant dose-response relationship whereas no DNA damage was obtained in the stomach. 5-fluorouracil did not induce DNA damage in either liver or stomach.

  14. Use of a standardized JaCVAM in vivo rat comet assay protocol to assess the genotoxicity of three coded test compounds; ampicillin trihydrate, 1,2-dimethylhydrazine dihydrochloride, and N-nitrosodimethylamine.

    PubMed

    McNamee, J P; Bellier, P V

    2015-07-01

    As part of the Japanese Center for the Validation of Alternative Methods (JaCVAM)-initiative international validation study of the in vivo rat alkaline comet assay (comet assay), our laboratory examined ampicillin trihydrate (AMP), 1,2-dimethylhydrazine dihydrochloride (DMH), and N-nitrosodimethylamine (NDA) using a standard comet assay validation protocol (v14.2) developed by the JaCVAM validation management team (VMT). Coded samples were received by our laboratory along with basic MSDS information. Solubility analysis and range-finding experiments of the coded test compounds were conducted for dose selection. Animal dosing schedules, the comet assay processing and analysis, and statistical analysis were conducted in accordance with the standard protocol. Based upon our blinded evaluation, AMP was not found to exhibit evidence of genotoxicity in either the rat liver or stomach. However, both NDA and DMH were observed to cause a significant increase in % tail DNA in the rat liver at all dose levels tested. While acute hepatoxicity was observed for these compounds in the high dose group, in the investigators opinion there were a sufficient number of consistently damaged/measurable cells at the medium and low dose groups to judge these compounds as genotoxic. There was no evidence of genotoxicity from either NDA or DMH in the rat stomach. In conclusion, our laboratory observed increased DNA damage from two blinded test compounds in rat liver (later identified as genotoxic carcinogens), while no evidence of genotoxicity was observed for the third blinded test compound (later identified as a non-genotoxic, non-carcinogen). This data supports the use of a standardized protocol of the in vivo comet assay as a cost-effective alternative genotoxicity assay for regulatory testing purposes.

  15. Case Series of HIV Infection-Associated Arteriopathy: Diagnosis, Management, and Outcome Over a 5-Year period at Maharaj Nakorn Chiang Mai Hospital, Chiang Mai University.

    PubMed

    Orrapin, Saritphat; Reanpang, Termpong; Orrapin, Saranat; Arwon, Supapong; Kattipathanapong, Thanate; Lekwanavijit, Suree; Rerkasem, Kittipan

    2015-09-01

    Patients infected with human immunodeficiency virus (HIV) can present with 4 pathology types: drug-induced vasospasm (ergotism), arterial limb ischemia, critical limb ischemia, and aneurysm. Although these problems are common vascular problems, they result in increased morbidity and mortality in HIV-infected patients, especially aneurysm. Patients with these problems tend to be diagnosed with difficulty because of atypical symptoms and signs. Because of lack of data in treatment outcome literature, our report explores and provides information on HIV infection-related arteriopathy. There were 17 patients in our 5-year review. There was no death in patients except the aneurysm type. The survival of aneurysm patients was significantly lower than from other pathologies (P = .003). Our case series showed good short-term outcome, and patients were not at risk for less beneficial surgical procedures.

  16. Setting Goals and Objectives for LD Children-Process and Problems

    ERIC Educational Resources Information Center

    Gallistel, Elizabeth R.

    1978-01-01

    Discussed are problems and procedures in setting goals and objectives for learning disabled children in the implementation of Individualized Education Programs required by the Education for All Handicapped Children Act. (Author/ DLS)

  17. Students Teaching Texts to Students: Integrating LdL and Digital Archives

    ERIC Educational Resources Information Center

    Stymeist, David

    2015-01-01

    The arrival of the digital age has not only reshaped and refocused critical research in the humanities, but has provided real opportunities to innovate with pedagogy and classroom structure. This article describes the development of a new pedagogical model that integrates learning by teaching with student access to electronic archival resources.…

  18. LD-pumped acousto-optical Q-switched burst-mode Nd:YAG laser

    NASA Astrophysics Data System (ADS)

    Pan, Hu; Yan, Renpeng; Li, Xudong; Ma, Yufei; Yu, Xin; Chen, Deying

    2016-04-01

    A high-repetition-rate, high-peak-power burst-mode laser for laser-based measurement applications is presented by using a master oscillator power amplifier structure. An laser diode arrays (LDA) side-pumped Nd:YAG acousto-optical (A-O) Q-switched laser serves as the master oscillator. Under pulsed pumping, pulse trains with 2-25 pulses are obtained when the repetition rate changes from 10 kHz to 100 kHz. The maximum pulse burst energy of 31.2 mJ is achieved in the A-O Q-switched pulse burst laser oscillator at 10 kHz. Two LDA side pumped Nd:YAG modules are employed in the amplification stage. After the amplification, the pulse burst energy at 10 kHz reaches ~170 mJ with a single pulse energy of 85.2 mJ and a pulse width of 14.5 ns, generating a peak power of 6.1 MW. At 100 kHz, the total burst energy reaches 220 mJ with a single pulse energy of 8.8 mJ in the pulse burst laser system.

  19. LD/ESL Assessments. NetNews. Volume 6, Number 2

    ERIC Educational Resources Information Center

    LDA of Minnesota, 2006

    2006-01-01

    The population of most Minnesota communities has dramatically changed over the past ten years. The Minnesota State Demographic Center reports the Twin Cities area has the largest Hmong, Somali, and Liberian communities in the United States. In some communities where both jobs and affordable housing are available, the percentage of English as a…

  20. Multi-marker-LD based genetic algorithm for tag SNP selection.

    PubMed

    Mouawad, Amer E; Mansour, Nashat

    2014-12-01

    Despite the advances in genotyping technologies which have led to large reduction in genotyping cost, the Tag SNP Selection problem remains an important problem for computational biologists and geneticists. Selecting the smallest subset of tag SNPs that can predict the other SNPs would considerably minimize the complexity of genome-wide or block-based SNP-disease association studies. These studies would lead to better diagnosis and treatment of diseases. In this work, we propose three variations of a genetic algorithm based on two-marker linkage disequilibrium, multi-marker linkage disequilibrium, and a third measure that we denote by prediction power. The performance of the three algorithms are compared with those of a recognized tag SNP selection algorithm using three different real data sets from the HapMap project. The results indicate that the multi-marker linkage disequilibrium based genetic algorithm yields better prediction accuracy.

  1. Life Span Transitions for a Student with LD: Mother-Daughter Perspectives

    ERIC Educational Resources Information Center

    Cumming, Therese M.; Smedley, Emily N.

    2017-01-01

    Transition planning and processes can greatly influence the quality of schooling transitions for students with learning disabilities. Student-focused planning and family involvement are two integral components of transition planning that do not always receive the emphasis that they deserve. The educational transitions of one student are examined…

  2. Computer-Based Graphic Organizers for Students with LD: A Systematic Review of Literature

    ERIC Educational Resources Information Center

    Ciullo, Stephen; Reutebuch, Colleen

    2013-01-01

    This article presents a systematic review of the literature for studies that utilized computer-based graphic organizers for students with learning disabilities. A comprehensive search yielded 12 studies that were coded and analyzed. The authors investigated the effectiveness of the treatments on academic outcomes, and selected integral…

  3. Working Memory in Dutch Children with Reading- and Arithmetic-Related LD

    ERIC Educational Resources Information Center

    van der Sluis, Sophie; van der Leij, Aryan; de Jong, Peter F.

    2005-01-01

    The aim of the two studies presented in this article was to examine working memory performance in Dutch children with various subtypes of learning disabilities. The performance of children with reading disabilities (RD) was compared to that of children with arithmetic disabilities (AD), children with both reading and arithmetic disabilities (RAD),…

  4. Applying LD Documentation Guidelines at the Postsecondary Level: Decision Making with Sparse or Missing Data

    ERIC Educational Resources Information Center

    Banerjee, Manju; Madaus, Joseph W.; Gelbar, Nicholas

    2015-01-01

    A key issue in fostering transition to postsecondary education for students with disabilities is documentation verifying the nature of the disability and supporting the need for services and reasonable accommodations. Documentation guidelines assist postsecondary disability service providers in making decisions about eligibility and reasonable…

  5. A Comparison of Eligibility Criteria and Their Impact on Minority Representation in LD Programs.

    ERIC Educational Resources Information Center

    Colarusso, Ronald P.; Keel, Marie C.; Dangel, Harry L.

    2001-01-01

    A review of 6,036 learning disabilities eligibility reports found the application of a regression equation to a standard score formula and the use of two different low-achievement cut-off criteria significantly increased the number of eligible white and African-American students, but neither alternative equalized the proportion of white to…

  6. Strategies for Enhancing the Performance of Students with LD in Inclusive Science Classes.

    ERIC Educational Resources Information Center

    Munk, Dennis D.; Bruckert, Jana; Call, Deborah T.; Stoehrmann, Traci; Radandt, Erin

    1998-01-01

    Provides an overview of curricular and instructional adaptations, modifications, and methods that have been used to provide science instruction to students with learning disabilities. Strategies are described within the context of a textbook-based unit on the solar system. (Author/DB)

  7. Learning Disabilities in New Zealand: Where Kiwis and Kids with LD Can't Fly.

    ERIC Educational Resources Information Center

    Chapman, James W.

    1992-01-01

    This article discusses reasons for rejection of the learning disability category in New Zealand and describes the general education and special education systems in New Zealand. It is suggested that the needs of such students may potentially be met by new policies for "high incidence" special needs. Teacher training needs are also…

  8. Welding-induced alignment distortion in DIP LD packages: effect of laser welding sequence

    NASA Astrophysics Data System (ADS)

    Liu, Wenning; Lin, Yaomin; Shi, Frank G.

    2002-06-01

    In pigtailing of a single mode fiber to a semiconductor laser for optical communication applications, the tolerance for displacement of the fiber relative to the laser is extremely tight, a submicron movement can often lead to a significant misalignment and thus the reduction in the power coupled into the fiber. Among various fiber pigtailing assembly technologies, pulsed laser welding is the method with submicron accuracy and is most conducive to automation. However, the melting-solidification process during laser welding can often distort the pre-achieved fiber-optic alignment. This Welding-Induced-Alignment-Distortion (WIAD) is a serious concern and significantly affects the yield for single mode fiber pigtailing to a semiconductor laser. This work presents a method for predicting WIAD as a function of various processing, laser, tooling and materials parameters. More specifically, the degree of WIAD produced by the laser welding in a dual-in-line laser diode package is predicted for the first time. An optimal welding sequence is obtained for minimizing WIAD.

  9. LdShake: Learning Design Solutions Sharing and Co-Edition

    ERIC Educational Resources Information Center

    Hernandez-Leo, Davinia; Romeo, Lauren; Carralero, Miguel A.; Chacon, Jonathan; Carrio, Mar; Moreno, Pau; Blat, Josep

    2011-01-01

    Two important challenges that teachers are currently facing are the sharing and the collaborative authoring of their learning design solutions, such as didactical units and learning materials. On the one hand, there are tools that can be used for the creation of design solutions and only some of them facilitate the co-edition. However, they do not…

  10. Adolescents with and without LD make the transition to middle school.

    PubMed

    Forgan, J W; Vaughn, S

    2000-01-01

    The purpose of this 2-year study was to describe how Hispanic students with and without learning disabilities fared academically and socially during the transition from elementary school to middle school. Participants were 14 Hispanic students who were instructed in a sixth-grade consultation/collaboration, inclusive elementary classroom before making the transition to middle school. Examination of social, academic, and student perception data revealed that, on the whole, the students with learning disabilities and those without experienced the transition similarly and fared well during the elementary-to-middle-school transition.

  11. ERDC-CERL LD-870 Download Program Developed for Aberdeen Test Center: User’s Manual

    DTIC Science & Technology

    2007-05-01

    noise monitors. It provides a trouble- shooting guide for resolving known issues with the monitoring system and contains information regarding modem...On-hook – A telephone is on-hook when its receiver is placed in the cra - dle. The telephone is not using the transmission line in this state and is

  12. Effects of Computer-Presented Text on LD Adolescents' Reading Behaviors.

    ERIC Educational Resources Information Center

    Keene, Sylvia; Davey, Beth

    1987-01-01

    Fifty-one learning disabled high school students read two lengthy expository passages from a computer monitor or comparably formatted printed page. Although the computer-displayed format did not affect comprehension processing, certain strategic behaviors, or time on task, it facilitated the lookback text strategy and improved attitudes toward the…

  13. Interviewing: A Technique for Discovering LD Adolescents' Strategies for Solving Multiplication Facts.

    ERIC Educational Resources Information Center

    Pieper, Edward

    1983-01-01

    The article describes the oral interview, a mathematics assessment technique in which the learning disabled adolescent's thinking stragegies are examined. Both efficient and inefficient thinking strategies related to multiplication are discussed, and implications for instruction considered. (Author/CL)

  14. Differential Diagnosis of Dysgraphia, Dyslexia, and OWL LD: Behavioral and Neuroimaging Evidence

    ERIC Educational Resources Information Center

    Berninger, Virginia W.; Richards, Todd L.; Abbott, Robert D.

    2015-01-01

    In Study 1, children in grades 4-9 (N = 88, 29 females and 59 males) with persisting reading and/or writing disabilities, despite considerable prior specialized instruction in and out of school, were given an evidence-based comprehensive assessment battery at the university while parents completed questionnaires regarding past and current history…

  15. The Underlying Message in LD Intervention Research: Findings from Research Syntheses.

    ERIC Educational Resources Information Center

    Vaughn, Sharon; Gersten, Russell; Chard, David J.

    2000-01-01

    This article summarizes the critical findings of recent research syntheses concerning intervention with students who have learning disabilities. The syntheses examined research on higher-order processing and problem- solving, reading comprehension, written expression, and grouping practices associated with improved outcomes in reading. Principles…

  16. QTL detection for a medium density SNP panel: comparison of different LD and LA methods

    PubMed Central

    Filangi, Olivier; Le Roy, Pascale

    2010-01-01

    Background New molecular technologies allow high throughput genotyping for QTL mapping with dense genetic maps. Therefore, the interest of linkage analysis models against linkage disequilibrium could be questioned. As these two strategies are very sensitive to marker density, experimental design structures, linkage disequilibrium extent and QTL effect, we propose to investigate these parameters effects on QTL detection. Methods The XIIIth QTLMAS workshop simulated dataset was analysed using three linkage disequilibrium models and a linkage analysis model. Interval mapping, multivariate and interaction between QTL analyses were performed using QTLMAP. Results The linkage analysis models identified 13 QTL, from which 10 mapped close of the 18 which were simulated and three other positions being falsely mapped as containing a QTL. Most of the QTLs identified by interval mapping analysis are not clearly detected by any linkage disequilibrium model. In addition, QTL effects are evolving during the time which was not observed using the linkage disequilibrium models. Conclusions Our results show that for such a marker density the interval mapping strategy is still better than using the linkage disequilibrium only. While the experimental design structure gives a lot of power to both approaches, the marker density and informativity clearly affect linkage disequilibrium efficiency for QTL detection. PMID:20380753

  17. Process and Product Explanations for What Works for LD and Why

    ERIC Educational Resources Information Center

    Womack, Sid T.; Hanna, Shellie L.; Woodall, Peggy; Callaway, Rebecca

    2011-01-01

    In the history of learning disabilities education, practitioners and researchers alike have been mired in controversies about product and process approaches. This review of literature attempts to identify the most useful research about product and process approaches and to suggest situations in which each may be most appropriate. Some literature…

  18. History as Narrative: The Nature and Quality of Historical Understanding for Students with LD

    ERIC Educational Resources Information Center

    Espin, Christine A.; Cevasco, Jazmin; van den Broek, Paul; Baker, Scott; Gersten, Russell

    2007-01-01

    In this study, we examine the nature and quality of students' comprehension of history. Specifically, we explore whether cognitive-psychological theories developed to capture the comprehension of narrative text can be used to capture the comprehension of history. Participants were 36 students with learning disabilities who had taken part in an…

  19. Rehabilitation Counselors and Postsecondary Academic Institutions: Partners in Meeting the Needs of LD College Students.

    ERIC Educational Resources Information Center

    Satcher, Jamie F.; Dooley-Dickey, Katherine

    This paper focuses on the joint partnership between the rehabilitation professional and postsecondary academic institutions when serving clients with learning disabilities. Definitions of learning disability are explored as well as the types of evaluations used to determine if a learning disability exists. Assessment techniques are discussed. The…

  20. LD-pumped double-clad fiber single-frequency power amplifier

    NASA Astrophysics Data System (ADS)

    Sun, Wen-Feng; Yang, Su-Hui; Zhao, Chang-Ming

    2005-12-01

    Single frequency, single mode laser output from a monolithic resonator was amplified by a double-clad D-shape fiber of 4.4 meters long. When the signal laser is 200mw, up to 6.65 W single frequency laser output was obtained, slope efficiency is 30.6%. The amplifier is Yb 3+ doped glass fiber pumped by a laser diode array at 976nm with signal at 1064nm. Single frequency amplification has been proved by a Fabri-Parrot interferometer. It is shown from the experiments that the signal input has not been saturated. By increasing the input signal, amplification can be increased further under the same pumping power. Experimental results meet well with theoretical calculation.

  1. Training, L&D, OD, HRD--What's in a Name?

    ERIC Educational Resources Information Center

    O'Toole, Sean

    2010-01-01

    This article describes the various aspects of human resources development and highlights the intersections and the differences between what are often mistakenly viewed as interchangeable concepts. It argues that, while it is generally accepted that developing staff is fundamental to good organisational health and business outcomes, a clear…

  2. InstanceCollage: A Tool for the Particularization of Collaborative IMS-LD Scripts

    ERIC Educational Resources Information Center

    Villasclaras-Fernandez, Eloy D.; Hernandez-Gonzalo, Julio A.; Hernandez-Leo, Davinia; Asensio-Perez, Juan I.; Dimitriadis, Yannis; Martinez-Mones, Alejandra

    2009-01-01

    Current research work in e-learning and more specifically in the field of CSCL (Computer Supported Collaborative Learning) deals with design of collaborative activities, according to computer-interpretable specifications, such as IMS Learning Design, and their posterior enactment using LMSs (Learning Management Systems). A script that describes…

  3. Source Interpretation: Teaching Students with and without LD to Read and Write Historically

    ERIC Educational Resources Information Center

    De La Paz, Susan; Morales, Petra; Winston, Philip M.

    2007-01-01

    We review domain-specific teaching approaches for students with learning disabilities in social studies, then present De La Paz's 2005 historical reasoning strategy so that readers understand the rationale for and have information on each stage of instruction. Next, we highlight the role of self-regulation for the reasoning process. We then turn…

  4. Source interpretation: teaching students with and without LD to read and write historically.

    PubMed

    De La Paz, Susan; Morales, Petra; Winston, Philip M

    2007-01-01

    We review domain-specific teaching approaches for students with learning disabilities in social studies, then present De La Paz's 2005 historical reasoning strategy so that readers understand the rationale for and have information on each stage of instruction. Next, we highlight the role of self-regulation for the reasoning process. We then turn to describing the role of each teacher (Morales as the social studies educator and Winston as the special educator), first as they collaborate with De La Paz and each other, then as Morales works alone, 1 year later. Implications are given for changes in teaching. The topic of women's suffrage is used throughout the article to highlight the teaching approach and to present student work.

  5. Free fat interpositional graft in acute physeal injuries: the anticipatory Langenskiöld procedure.

    PubMed

    Foster, B K; John, B; Hasler, C

    2000-01-01

    Free fat graft interposition has been used extensively in management of physeal injuries with established growth disturbances. The use of this technique as part of the management of acute physeal injuries has not been reported. Here we report on its application in acute physeal injuries, where it has prevented the formation of an anticipated physeal arrest.

  6. LD-pumped erbium and neodymium lasers with high energy and output beam quality

    NASA Astrophysics Data System (ADS)

    Kabanov, Vladimir V.; Bezyazychnaya, Tatiana V.; Bogdanovich, Maxim V.; Grigor'ev, Alexandr V.; Lebiadok, Yahor V.; Lepchenkov, Kirill V.; Ryabtsev, Andrew G.; Ryabtsev, Gennadii I.; Shchemelev, Maxim A.

    2013-05-01

    Physical and fabrication peculiarities which provide the high output energy and beam quality for the diode pumped erbium glass and Nd:YAG lasers are considered. Developed design approach allow to make passively Q-switched erbium glass eye-safe portable laser sources with output energy 8 - 12 mJ (output pulse duration is less than 25 ns, pulse repetition rate up to 5 Hz) and beam quality M2 less than 1.3. To reach these values the erbium laser pump unit parameters were optimized also. Namely, for the powerful laser diode arrays the optimal near-field fill-factor, output mirror reflectivity and heterostructure properties were determined. Construction of advanced diode and solid-state lasers as well as the optical properties of the active element and the pump unit make possible the lasing within a rather wide temperature interval (e.g. from minus forty till plus sixty Celsius degree) without application of water-based chillers. The transversally pumped Nd:YAG laser output beam uniformity was investigated depending on the active element (AE) pump conditions. In particular, to enhance the pump uniformity within AE volume, a special layer which practically doesn't absorb the pump radiation but effectively scatters the pump and lasing beams, was used. Application of such layer results in amplified spontaneous emission suppression and improvement of the laser output beam uniformity. The carried out investigations allow us to fabricate the solid-state Nd:YAG lasers (1064 nm) with the output energy up to 420 mJ at the pulse repetition rate up to 30 Hz and the output energy up to 100 mJ at the pulse repetition rate of of 100 Hz. Also the laser sources with following characteristics: 35 mJ, 30 Hz (266 nm); 60 mJ, 30 Hz (355 nm); 100 mJ, 30 Hz (532 nm) were manufactured on the base of the developed Nd:YAG quantrons.

  7. Self-Determination Perceptions and Behaviors of Diverse Students with LD During the Transition Planning Process

    ERIC Educational Resources Information Center

    Trainor, Audrey A.

    2005-01-01

    Transition models include components of student self-determination during transition planning meetings. Researchers acknowledge that cultural identity may influence both transition decisions and self-determination strategies. Yet the appropriateness of these approaches for culturally and linguistically diverse students with learning disabilities…

  8. Using Evidence-Based Multimedia to Improve Vocabulary Performance of Adolescents With LD: A UDL Approach

    ERIC Educational Resources Information Center

    Kennedy, Michael J.; Thomas, Cathy Newman; Meyer, J. Patrick; Alves, Kat D.; Lloyd, John Wills

    2014-01-01

    Universal Design for Learning (UDL) is a framework that is commonly used for guiding the construction and delivery of instruction intended to support all students. In this study, we used a related model to guide creation of a multimedia-based instructional tool called content acquisition podcasts (CAPs). CAPs delivered vocabulary instruction…

  9. An Update on NCAA College Freshman Academic Requirements: The Impact on Students with LD.

    ERIC Educational Resources Information Center

    Hishinuma, Earl S.

    1999-01-01

    Analyzes the consent decree settling the dispute between the National Collegiate Athletic Association and the Department of Justice relating to the eligibility of students with learning disabilities for participation in college freshman athletics. Potential problems in the settlement are identified, as are such issues as NCAA's denial that it is…

  10. Acute Oral Toxicity (LD50) of 4-Nitrophenyl Monochloromethyl (Phenyl) Phosphinate (TA009) in Male Rats

    DTIC Science & Technology

    1984-10-01

    phosphinates hydrolyze readily in aqueous solutions, a vehicle which would minimize the rate of hydrolysis was required. A mixture of Tween 80 (Fisher...monochloroiethyl (phenyl) phosphinat_. formulated with Tween 80 , EtOH, and citrate buffer (LAIR SOP-OP-STX-45, Preparation of Compounds Unstable in Water...phosphinate. 16.0 ml Tween 80 8.0 ml (100 %) ethanol, 56.0 ml citrate buffer (50 mM) at a pH of 3.2. The vehicle was the same as above without phosphinate. pH

  11. Acute Oral Toxicity (LD50) of 4-Nitrophenyl Monochloromethyl (Phenyl) Phosphinate (TA009) in Female Rats

    DTIC Science & Technology

    1984-10-01

    chosen was a mixture of Tween 80 (Fisher Scientific Company, Fairlawn, NJ) ethanol and citrate buffer (pH 2.9). Additional information on the vehicle...phosphinate formulated with Tween 80 , EtOH, and citrate buffer (LAIR SOP-OP-STX-45, Preparation of Compounds Unstable in Water for SLRL Assay). A 2.0...percent phosphinate solution was prepared with 1.5 g 4- nitrophenyl monochloromethyl (phenyl) phosphinate. 15.0 ml Tween 80 7.5 ml (100 %) ethanol

  12. PIAA Coronagraph Development at NASA Ames: High Contrast Laboratory Demonstration at 2 l/D

    NASA Astrophysics Data System (ADS)

    Belikov, Ruslan; Pluzhnik, E.; Witteborn, F. C.; Lynch, D. H.; Greene, T. P.; Zell, P. T.; Balasubramanian, K.; Guyon, O.

    2011-01-01

    Coronagraph technology is advancing and promises to directly image and spectrally characterize extrasolar Earth-like planets in the foreseeable future (such as the 2020 decade) with a telescope as small as 1.5m. A small Explorer-sized telescope can also be launched in the 2010 decade capable of seeing debris disks as small as 10s of zodis and potentially a few large planets. The Phase Induced Amplitude Apodization (PIAA) coronagraph makes such aggressive performance possible. We report on the latest results from a testbed at NASA Ames that is focused on developing and testing the PIAA coronagraph. This laboratory facility was built in 2008 and is designed to be flexible, operated in an actively thermally stabilized air environment, and to complement collaborative efforts at NASA JPL's High Contrast Imaging Testbed. For our wavefront control we are using small Micro-Electro-Mechanical-System deformable mirrors (MEMS DMs), which promise to reduce the size of the beam and overall instrument, a consideration that becomes very important for small telescopes. We describe our lab efforts and results, which include: the operation of our new active thermal control system; the demonstration of 5.4x10-8 (at time of this writing) average raw contrast in a dark zone from 2.0 - 5.2 λ/D in monochromatic light with a refractive PIAA system; preliminary results with an innovative low-cost set of reflective PIAA from JPL; preliminary results with a set of next-generation reflective PIAA built by Tinsley and designed to have the best theoretical broadband performance so far; and finally, an innovative design for a chromatically compensated focal plane occulter that promises to enhance broadband performance by matching the wavelength-dependent inner working angle of coronagraphs such as PIAA.

  13. Knowledge Base for Automatic Generation of Online IMS LD Compliant Course Structures

    ERIC Educational Resources Information Center

    Pacurar, Ecaterina Giacomini; Trigano, Philippe; Alupoaie, Sorin

    2006-01-01

    Our article presents a pedagogical scenarios-based web application that allows the automatic generation and development of pedagogical websites. These pedagogical scenarios are represented in the IMS Learning Design standard. Our application is a web portal helping teachers to dynamically generate web course structures, to edit pedagogical content…

  14. Socially ADDept: Teaching Social Skills to Children with ADHD, LD, and Asperger's, Revised Edition

    ERIC Educational Resources Information Center

    Giler, Janet Z.

    2011-01-01

    "Socially ADDept" helps educators and parents teach the hidden rules of social behavior to children with limited social skills, notably those with special needs like ADHD, learning disabilities, Asperger's and high-functioning autism, Tourette Syndrome, and nonverbal learning disabilities. The author provides all the information parents and…

  15. Evaluation of 4,4'-diaminodiphenyl ether in the rat comet assay: Part of the Japanese Center for the Validation of Alternative Methods (JaCVAM)-initiative international validation study of in vivo rat alkaline comet assay.

    PubMed

    Priestley, Catherine C; Walker, Joanne S; O'Donovan, Michael R; Doherty, Ann T

    2015-07-01

    As a part of the Japanese Center for the Validation of Alternative Methods (JaCVAM)-initiative international validation study of the in vivo rat alkaline comet assay, 4,4'-diaminodiphenyl ether (DPE), a known rodent genotoxic carcinogen, was tested in this laboratory. Sprague Dawley rats (7-9 weeks of age) were given three oral doses of DPE, 24 and 21 h apart and liver or stomach sampled 3h after the final dose. Under the conditions of the test, no increases in DNA damage in liver and stomach were observed with DPE (up to 200 mg/kg/day). A dose-dependent decrease in DNA migration, compared to vehicle controls, was noted for DPE in rat stomach. Further analysis is required to elucidate fully whether this decrease is a consequence of the mode of action or due to the toxicity of DPE. What is perhaps surprising is the inability of the comet assay to detect a known rat genotoxic carcinogen in liver. Further investigation is needed to clarify whether this apparent lack of response results from limited tissue exposure or metabolic differences between species. This finding highlights a need for careful consideration of study design when evaluating assay performance as a measure of in vivo genotoxicity.

  16. JaCVAM-organized international validation study of the in vivo rodent alkaline comet assay for the detection of genotoxic carcinogens: I. Summary of pre-validation study results.

    PubMed

    Uno, Yoshifumi; Kojima, Hajime; Omori, Takashi; Corvi, Raffaella; Honma, Masamistu; Schechtman, Leonard M; Tice, Raymond R; Burlinson, Brian; Escobar, Patricia A; Kraynak, Andrew R; Nakagawa, Yuzuki; Nakajima, Madoka; Pant, Kamala; Asano, Norihide; Lovell, David; Morita, Takeshi; Ohno, Yasuo; Hayashi, Makoto

    2015-07-01

    The in vivo rodent alkaline comet assay (comet assay) is used internationally to investigate the in vivo genotoxic potential of test chemicals. This assay, however, has not previously been formally validated. The Japanese Center for the Validation of Alternative Methods (JaCVAM), with the cooperation of the U.S. NTP Interagency Center for the Evaluation of Alternative Toxicological Methods (NICEATM)/the Interagency Coordinating Committee on the Validation of Alternative Methods (ICCVAM), the European Centre for the Validation of Alternative Methods (ECVAM), and the Japanese Environmental Mutagen Society/Mammalian Mutagenesis Study Group (JEMS/MMS), organized an international validation study to evaluate the reliability and relevance of the assay for identifying genotoxic carcinogens, using liver and stomach as target organs. The ultimate goal of this validation effort was to establish an Organisation for Economic Co-operation and Development (OECD) test guideline. The purpose of the pre-validation studies (i.e., Phase 1 through 3), conducted in four or five laboratories with extensive comet assay experience, was to optimize the protocol to be used during the definitive validation study.

  17. The analysis of MAI in large scale MIMO-CDMA system

    NASA Astrophysics Data System (ADS)

    Berceanu, Madalina-Georgiana; Voicu, Carmen; Halunga, Simona

    2016-12-01

    Recently, technological development imposed a rapid growth in the use of data carried by cellular services, which also implies the necessity of higher data rates and lower latency. To meet the users' demands, it was brought into discussion a series of new data processing techniques. In this paper, we approached the MIMO technology that uses multiple antennas at the receiver and transmitter ends. To study the performances obtained by this technology, we proposed a MIMO-CDMA system, where image transmission has been used instead of random data transmission to take benefit of a larger range of quality indicators. In the simulations we increased the number of antennas, we observed how the performances of the system are modified and, based on that, we were able to make a comparison between a conventional MIMO and a Large Scale MIMO system, in terms of BER and MSSIM index, which is a metric that compares the quality of the image before transmission with the received one.

  18. Mandatory Procedures for Major Defense Acquisition Programs (MDAPS) and Major Automated Information System (MAIS) Acquisition Programs

    DTIC Science & Technology

    2002-04-05

    ACQUISITION STRATEGY 32 C2.8. SUPPORT STRATEGY 34 DoD 5000.2-R, April 5, 2002 TABLE OF CONTENTS 5 C2.9. BUSINESS STRATEGY 40 C3. CHAPTER 3 TEST AND...AL1.1.110. PA&E Program Analysis and Evaluation AL1.1.111. PAUC Program Acquisition Unit Cost AL1.1.112. PBBE Performance-Based Business Environment...Simulation-Based Acquisition AL1.1.132. SBIR Small Business Innovation Research AL1.1.133. SEI Software Engineering Institute AL1.1.134. SSAA System

  19. E Lauhoe Mai Na Wa'a: Toward a Hawaiian Indigenous Education Teaching Framework

    ERIC Educational Resources Information Center

    Kana'iaupuni, Shawn Malia; Kawai'ae'a, Keiki K. C.

    2008-01-01

    The desire to improve educational delivery and outcomes has prompted significant advancements in culture-based education as a foundation for community-driven, place-based, relevant educational approaches that more effectively engage children and their families in lifelong learning and leadership. This article shares the early process and tools of…

  20. Campylobacter jejuni in hospitalized children with diarrhoea in Chiang Mai, Thailand.

    PubMed

    Poocharoen, L; Bruin, C W

    1986-03-01

    In this hospital based study, Campylobacter jejuni, although endemic in Northern Thailand was not found in a significantly higher percentage of diarrhoea than in control children. It was isolated from the stools of 14 of 208 diarrhoea (6.7%) and 6 of 108 (5.5%) control patients. Ten of the 14 positive diarrhoea cases were mixed infections, other pathogens isolated simultaneously were enteropathogenic E. coli 4x, Shigella spp. 3x, Salmonella spp. 4x, rotavirus 3x, Plesiomonas shigelloides 1x and parasites 3x. Infection with Campylobacter jejuni occurred mainly in the 1-2 year age group and was not found in patients over 5. It was distributed over the year without seasonal peaks. In four of the 14 positive C. jejuni cases no other pathogens were found. One of these suffered 2nd degree malnutrition with measles and one was a chronic diarrhoea case with a history of antibiotic use and past Salmonella and Shigella infections. The remaining two cases had no underlying or associated illnesses. It was concluded that Campylobacter jejuni may be important as an associated pathogen in complicated diarrhoea infections but is rare as a sole causative agent.

  1. Huakina Mai: A Kaupapa Maori Approach to Relationship and Behaviour Support

    ERIC Educational Resources Information Center

    Savage, Catherine; Macfarlane, Sonja; Macfarlane, Angus; Fickel, Letitia; Hemi, Hemi Te

    2014-01-01

    This article presents the developmental stages of a nationwide whole-school strengths-based behavioural intervention by Maori and centring on Maori interests; an initiative that has the potential to transform educational success and opportunities. The initial phase involved a cycle of data collection. This was conducted via a series of focus…

  2. NGA Tukitanga Mai Koka Ki Tona Ira: Maori mothers and child to mother violence.

    PubMed

    Ryan, Ripekapaia Gloria; Wilson, Denise

    2010-11-01

    In common with other indigenous women Māori mothers risk illness, harm, and possible death when abused and intimidated by their children. Yet women suffering child to mother violence are silenced by their fear and shame, and endeavour to minimise the effects of this form of abuse. A qualitative descriptive research design using kaupapa Māori methodology was adopted to explore the experiences of Māori mothers who had been abused by a son or daughter. During semi-structured interviews with five Māori women experiences of abuse by a child, and its impact on the whānau/family were recorded. Interview transcripts were analysed thematically, and three key themes emerged: behind closed doors, my child and a new journey. These Māri mothers carried the secret of the violence alone; keeping it behind closed doors while paradoxically protecting their abusive child. Nonetheless, these mothers reached a point where they chose to undertake a new journey, one that involved telling their story, reconnecting with their indigenous roots, and engaging in healing activities. The mothers' experiences highlight a lack of support and responsiveness by support and health agencies. Regardless of these negative experiences with support agencies, we highlight the important role nurses have in facilitating whānau ora (family wellbeing) for these women. This research contributes an indigenous perspective to the growing literature on child to mother violence, and provides direction for future research.

  3. Spondylodiscite granulomateuse: surtout la tuberculose mais ne pas omettre le lymphome

    PubMed Central

    Zinebi, Ali; Rkiouak, Adil; Akhouad, Yousef; Reggad, Ahmed; Kasmy, Zohour; Boudlal, Mostafa; Lho, Abdelhamid Nait; Rabhi, Moncef; Sinaa, Mohamed; Ennibi, Khalid; Chaari, Jilali

    2016-01-01

    Les douleurs lombaires relèvent d'étiologies multiples dont le diagnostic peut être source de grandes difficultés. Le lymphome rachidien primitif est rare et son diagnostic nécessite une biopsie souvent scanoguidée. Un homme de 30 ans, était hospitalisé pour lombalgies inflammatoires évoluant dans un contexte d'altération de l'état général avec à l'examen des douleurs à la palpation des apophyses épineux L2L3, sans syndrome tumoral périphérique. Le bilan biologique montrait un syndrome inflammatoire. Le bilan morphologique était en faveur d'une spondylodiscite. La première biopsie montrait une ostéite granulomateuse. L'aggravation clinique et radiologique sous anti bacillaire a mené à reconsidérer le diagnostic et la deuxième biopsie confirme le diagnostic du lymphome. Le diagnostic de tuberculose osseuse en particulier vertébrale nécessite une confirmation bactériologique et ou histologique pour ne pas méconnaître un lymphome osseux primitif. PMID:28292061

  4. Mais comment s'écoule donc un glacier ? Aperçu historique

    NASA Astrophysics Data System (ADS)

    Rémy, Frédérique; Testut, Laurent

    2006-05-01

    Ice and snow have often helped physicists understand the world. On the contrary it has taken them a very long time to understand the flow of the glaciers. Naturalists only began to take an interest in glaciers at the beginning of the 19th century during the last phase of glacier advances. When the glacier flow from the upslope direction became obvious, it was then necessary to understand how it flowed. It was only in 1840, the year of the Antarctica ice sheet discovery by Dumont d'Urville, that two books laid the basis for the future field of glaciology: one by Agassiz on the ice age and glaciers, the other one by canon Rendu on glacier theory. During the 19th century, ice flow theories, adopted by most of the leading scientists, were based on melting/refreezing processes. Even though the word 'fluid' was first used in 1773 to describe ice, more the 130 years would have to go by before the laws of fluid mechanics were applied to ice. Even now, the parameter of Glen's law, which is used by glaciologists to model ice deformation, can take a very wide range of values, so that no unique ice flow law has yet been defined. To cite this article: F. Rémy, L. Testut, C. R. Geoscience 338 (2006).

  5. Sirenomelia type VI (sympus apus) in one of dizygotic twins at Chiang Mai University Hospital.

    PubMed

    Nokeaingtong, Kwannapas; Kaewchai, Sirirat; Visrutaratna, Pannee; Khuwuthyakorn, Varangthip

    2015-05-14

    Those born with sirenomelia, a rare congenital anomaly, have features resembling a mermaid. Characteristics of sirenomelia are a single lower limb, sacral and pelvic bone defects, and anorectal and urogenital malformations. There is an increased incidence of sirenomelia in males and twins. This case was a preterm male, dizygotic twin and product of in vitro fertilisation. The baby was born by caesarean section due to breech presentation. He was found to have a fused lower extremity and absent external genitalia and anus. The baby passed away shortly after birth due to severe respiratory failure. Radiographic findings showed small lung volume and pneumothoraces. There were multiple segmental fusions of the vertebrae. Single femur and single tibia were presented in a fused lower limb. Autopsy demonstrated large intestinal atresia, intra-abdominal testes, absence of kidney, ureter and bladder, single umbilical artery, agenesis of blood vessels at lower extremity and agenesis of sacrum and coccyx.

  6. Computer Supported Indexing: A History and Evaluation of NASA's MAI System. Supplement 24

    NASA Technical Reports Server (NTRS)

    Silvester, June P.

    1997-01-01

    Computer supported indexing systems may be categorized in several ways. One classification scheme refers to them as statistical, syntactic, semantic or knowledge-based. While a system may emphasize one of these aspects, most systems actually combine two or more of these mechanisms to maximize system efficiency. Statistical systems can be based on counts of words or word stems, statistical association, and correlation techniques that assign weights to word locations or provide lexical disambiguation, calculations regarding the likelihood of word co-occurrences, clustering of word stems and transformations, or any other computational method used to identify pertinent terms. If words are counted, the ones of median frequency become candidate index terms. Syntactical systems stress grammar and identify parts of speech. Concepts found in designated grammatical combinations, such as noun phrases, generate the suggested terms. Semantic systems are concerned with the context sensitivity of words in text. The primary goal of this type of indexing is to identify without regard to syntax the subject matter and the context-bearing words in the text being indexed. Knowledge-based systems provide a conceptual network that goes past thesaurus or equivalent relationships to knowing (e.g., in the National Library of Medicine (NLM) system) that because the tibia is part of the leg, a document relating to injuries to the tibia should he indexed to LEG INJURIES, not the broader MeSH term INJURIES, or knowing that the term FEMALE should automatically be added when the term PREGNANCY is assigned, and also that the indexer should be prompted to add either HUMAN or ANIMAL. Another way of categorizing indexing systems is to identify them as producing either assigned- or derived-term indexes.

  7. A 3D-DNA Molecule Made of PlayMais

    ERIC Educational Resources Information Center

    Caine, Massimo; Horié, Ninon; Zuchuat, Sandrine; Weber, Aurélia; Ducret, Verena; Linder, Patrick; Perron, Karl

    2015-01-01

    More than 60 years have passed since the work of Rosalind Franklin, James Watson, and Francis Crick led to the discovery of the 3D-DNA double-helix structure. Nowadays, due to the simple and elegant architecture of its double helix, the structure of DNA is widely known. The biological role of the DNA molecule (e.g., genetic information), however,…

  8. Mandatory Procedures for Major Defense Acquisition Programs (MDAPs) and Major Automated Information System (MAIS) Acquisition Programs.

    DTIC Science & Technology

    2007-11-02

    at the subcontract levels will be developed. 3.3.1.1 Commercial and Non-Developmental Items Market research and analysis shall be conducted to...a military and a civil application. Market research and analysis should be conducted to identify and evaluate possible dual use technologies and

  9. Nutritional status and feeding practices in gastrointestinal surgery patients at Bach Mai Hospital, Hanoi, Vietnam.

    PubMed

    Young, Lorraine S; Huong, Pham Thi Thu; Lam, Nguyen Thi; Thu, Nghiem Nguyet; Van, Ha Thi; Hanh, Nguyen Lien; Tuyen, Le Danh; Lien, Dinh Thi Kim; Hoc, Tran Hieu; Tuyet, Chu Thi; Anh, Nguyen Quoc; Henry, Elizabeth G; Lenders, Carine M; Gura, Kathleen M; Bigornia, Sherman J; Apovian, Caroline M; Ziegler, Thomas R

    2016-01-01

    背景与目的:越南手术患者的营养状况和院内喂养方式没有被详细记录。根 据巴赫麦医院的一个横断面研究,在外科病房应用体质指数(BMI<18.5 kg/m2)所判定的营养不良患病率为33%。我们在越南河内巴赫麦医院的对胃 肠(GI)外科病房的喂养方法进行了观察研究。方法与研究设计:来自美国 和越南国家营养研究所的调查员从巴赫麦医院招募72 名入院进行择期胃肠手 术的患者参与了这项观察性研究。记录基线人体测量指标及其随时间的变化, 体质指数,主观整体评估(SGA),从入院到出院经口摄食、管饲喂养和肠外 营养的每日能量和蛋白质的摄入量。结果:共有50%的研究对象SGA 得分为 B 或C,48%的研究对象BMI<18.5,而平均的上臂围为正常水平偏低(24±4 cm)。几乎所有的患者(98%)在术后给予肠外营养,平均术后4 天给予经 口喂养,只有一个病人是管饲喂养。住院期间,平均每天总能量摄入为15 kcal/kg/day,蛋白质摄入为0.61 g/kg/day,接受肠外营养者极少补充微量元 素。结论:越南手术患者住院期间营养不良是一个重要问题,围手术期患者 出现喂养不理想和术后早期应用肠内营养是常规。.

  10. Ecology for the shrinking city (JA)

    EPA Science Inventory

    This article brings together the concepts of shrinking cities—the hundreds of cities worldwide experiencing long-term population loss—and ecology for the city. Ecology for the city is the application of a social–ecological understanding to shaping urban form and function along su...

  11. Personnel Management: A J/A Perspective

    ERIC Educational Resources Information Center

    Tasca, A. J.

    1974-01-01

    Recently, personnel executives and their staffs are being asked to help management solve an increasing number of human resource and business problems. Personnel management must undergo some changes if it is to achieve its full potential. (Author/AJ)

  12. Math Learning Disability and Math LD Subtypes: Evidence from Studies of Turner Syndrome, Fragile X Syndrome, and Neurofibromatosis Type 1.

    ERIC Educational Resources Information Center

    Mazzocco, Michele M. M.

    2001-01-01

    This study examined whether indicators of math learning disability were observed in 35 5- and 6-year-olds with either neurofibromatosis, Turner Syndrome, or fragile X syndrome and compared to controls. Findings indicate that girls with fragile X or Turner syndrome but not neurofibromatosis are significantly more likely to have specific math…

  13. Simulation and experiment of pump distribution in LD end-pumped Nd:YAG single crystal fiber

    NASA Astrophysics Data System (ADS)

    Cai, XiaoTian; Li, Xiao; Zhao, GuoMin

    2017-01-01

    The spread of the pump model, established based on MATLAB, simulates the distribution of the pump in End-Pumped single crystal fiber. Simulation results show that the pump in the rod single crystal fiber will converge again. By changing the crystal absorption coefficient, it can be found that smaller the absorption coefficient is, more uniform the pump distribution is; when it is greater, the pump will concentrate to the pump end more seriously. Establish End- Pumped Experimental platform in the experiment, the crystal is 1 mm in diameter and length of 30 mm, Nd3+ doping concentration is 1%. Change the position of the pump light's focus in the crystal, we can see different distribution of the pump light by different focus location in the crystal and find that the pump light has the most homogeneous distribution when the focus is on the crystal axis and has 1mm distance to the pump end face. At this time, the second convergence of the pump is clearly visible. By changing the pump wavelength, crystal absorption coefficient changes. It is found that under the same pump power, absorption coefficient is greater, the pump will concentrate to the pump end more seriously. And the temperature of crystal pump end rises, which is identical with the simulation results. The results indicate that for the single crystal fiber, the higher absorption coefficient is not better, low absorption coefficient leads to the uniform distribution of the pump, there will be a better absorption in a relatively long length of single crystal fiber. And due to the lower end face temperature, end pump power upper limit will also increase.

  14. Identification of putative candidate genes for red rot resistance in sugarcane (Saccharum species hybrid) using LD-based association mapping.

    PubMed

    Singh, Ram K; Banerjee, Nandita; Khan, M S; Yadav, Sonia; Kumar, Sanjeev; Duttamajumder, S K; Lal, Ram Ji; Patel, Jinesh D; Guo, H; Zhang, Dong; Paterson, Andrew H

    2016-06-01

    Red rot is a serious disease of sugarcane caused by the fungus Colletotrichum falcatum that has a colossal damage potential. The fungus, prevalent mainly in the Indian sub-continent, keeps on producing new pathogenic strains leading to breakdown of resistance in newly released varieties and hence the deployment of linked markers for marker-assisted selection for resistance to this disease can fine tune the breeding programme. This study based on a panel of 119 sugarcane genotypes fingerprinted for 944 SSR alleles was undertaken with an aim to identify marker-trait associations (MTAs) for resistance to red rot. Mixed linear model containing population structure and kinship as co-factor detected four MTAs that were able to explain 10-16 % of the trait variation, individually. Among the four MTAs, EST sequences diagnostic of three could be BLAST searched to the sorghum genome with significant sequence homology. Several genes encoding important plant defence related proteins, viz., cytochrome P450, Glycerol-3-phosphate transporter-1, MAP Kinase-4, Serine/threonine-protein kinase, Ring finger domain protein and others were localized to the vicinity of these MTAs. These positional candidate genes are worth of further investigation and possibly these could contribute directly to red rot resistance, and may find a potential application in marker-assisted sugarcane breeding.

  15. High-peak-power, high-repetition-rate LD end-pumped Nd:YVO4 burst mode laser

    NASA Astrophysics Data System (ADS)

    Pan, Hu; Yan, Renpeng; Fa, Xin; Yu, Xin; Ma, Yufei; Fan, Rongwei; Li, Xudong; Chen, Deying; Zhou, Zhongxiang

    2016-06-01

    A compact high-peak-power, high-repetition-rate burst mode laser is achieved by an acousto-optical Q-switched Nd:YVO4 1064 nm laser directly pumped at 878.6 nm. Pulse trains with 10-100 pulses are obtained using acousto-optical Q-switch at repetition rates of 10-100 kHz under a pulsed pumping with a 1 ms duration. At the maximum pump energy of 108.5 mJ, the pulse energy of 10 kHz burst mode laser reaches 44 mJ corresponding to a single pulse energy of 4.4 mJ and an optical-to-optical efficiency of 40.5 %.The maximum peak power of ~468.1 kW at 10 kHz is obtained with a pulse width of 9.4 ns. The beam quality factor is measured to be M 2 ~1.5 and the pulse jitter is estimated to be less than 1 % in both amplitude and time region.

  16. Modern Languages and Specific Learning Difficulties (SpLD): Implications of Teaching Adult Learners with Dyslexia in Distance Learning

    ERIC Educational Resources Information Center

    Gallardo, Matilde; Heiser, Sarah; Arias McLaughlin, Ximena

    2015-01-01

    In modern language (ML) distance learning programmes, teachers and students use online tools to facilitate, reinforce and support independent learning. This makes it essential for teachers to develop pedagogical expertise in using online communication tools to perform their role. Teachers frequently raise questions of how best to support the needs…

  17. Dermal, Eye and Oral Toxicological Evaluations. Phase II. Acute Oral LD Determinations of Benzothiazole, Dithiane, and Oxathiane.

    DTIC Science & Technology

    1986-09-03

    Dose - Response Curve for Males-Benzothiazole .............. 14 2. Dose - Response Curve for...Females-Benzothiazole .......... 15 3. Dose - Response Curve for Combined Sexes-Benzothiazole... 16 4. Dose - Response Curve for Males-Dithiane...29 5. Dose - Response Curve for Females-Dithiane ................. 30 6. Dose - Response Curve for Combined Sexes-Dithiane ........ 31 7. Dose

  18. High overlap of CNVs and selection signatures revealed by varLD analyses of taurine and zebu cattle

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Selection Signatures (SS) assessed through analysis of genomic data are being widely studied to discover population specific regions selected via artificial or natural selection. Different methodologies have been proposed for these analyses, each having specific limitations as to the age of the sele...

  19. The neonicotinoid pesticide, imidacloprid, affects Bombus impatiens (bumblebee) sonication behavior when consumed at doses below the LD50.

    PubMed

    Switzer, Callin M; Combes, Stacey A

    2016-08-01

    We investigated changes in sonication (or buzz-pollination) behavior of Bombus impatiens bumblebees, after consumption of the neonicotinoid pesticide, imidacloprid. We measured sonication frequency, sonication length, and flight (wing beat) frequency of marked bees collecting pollen from Solanum lycopsersicum (tomato), and then randomly assigned bees to consume 0, 0.0515, 0.515, or 5.15 ng of imidacloprid. We recorded the number of bees in each treatment group that resumed sonication behavior after consuming imidacloprid, and re-measured sonication and flight behavior for these bees. We did not find evidence that consuming 0.0515 ng imidacloprid affected the sonication length, sonication frequency, or flight frequency for bees that sonicated after consuming imidacloprid; we were unable to test changes in these variables for bees that consumed 0.515 or 5.15 ng because we did not observe enough of these bees sonicating after treatment. We performed Cox proportional hazard regression to determine whether consuming imidacloprid affected the probability of engaging in further sonication behavior on S. lycopersicum and found that bumblebees who consumed 0.515 or 5.15 ng of imidacloprid were significantly less likely to sonicate after treatment than bees who consumed no imidacloprid. At the end of the experiment, we classified bees as dead or alive; our data suggest a trend of increasing mortality with higher doses of imidacloprid. Our results show that even modest doses of imidacloprid can significantly affect the likelihood of bumblebees engaging in sonication, a behavior critical for the pollination of a variety of crops and other plants.

  20. A cognitive strategy instruction to improve math calculation for children with ADHD and LD: a randomized controlled study.

    PubMed

    Iseman, Jackie S; Naglieri, Jack A

    2011-01-01

    The authors examined the effectiveness of cognitive strategy instruction based on PASS (Planning, Attention, Simultaneous, Successive) given by special education teachers to students with ADHD randomly assigned by classroom. Students in the experimental group were exposed to a brief cognitive strategy instruction for 10 days, which was designed to encourage development and application of effective planning for mathematical computation, whereas the comparison group received-standard math instruction. Standardized tests of cognitive processes and math achievement were given at pretest. All students completed math worksheets throughout the experimental phase. Standardized achievement tests (Woodcock-Johnson Tests of Achievement, Third Edition, Math Fluency and Wechsler Individualized Achievement Test, Second Edition, Numerical Operations) were administered pre- and postintervention, and Math Fluency was also administered at 1 year follow-up. Large pre-post effect sizes were found for students in the experimental group but not the comparison group on math worksheets (0.85 and 0.26), Math Fluency (1.17 and 0.09), and Numerical Operations (0.40 and -0.14, respectively). At 1 year follow-up, the experimental group continued to outperform the comparison group. These findings suggest that students with ADHD evidenced greater improvement in math worksheets, far transfer to standardized tests of math (which measured the skill of generalizing learned strategies to other similar tasks), and continued advantage 1 year later when provided the PASS-based cognitive strategy instruction.

  1. Epidemic Clones, Oceanic Gene Pools, and Eco-LD in the Free Living Marine Pathogen Vibrio parahaemolyticus.

    PubMed

    Cui, Yujun; Yang, Xianwei; Didelot, Xavier; Guo, Chenyi; Li, Dongfang; Yan, Yanfeng; Zhang, Yiquan; Yuan, Yanting; Yang, Huanming; Wang, Jian; Wang, Jun; Song, Yajun; Zhou, Dongsheng; Falush, Daniel; Yang, Ruifu

    2015-06-01

    We investigated global patterns of variation in 157 whole-genome sequences of Vibrio parahaemolyticus, a free-living and seafood associated marine bacterium. Pandemic clones, responsible for recent outbreaks of gastroenteritis in humans, have spread globally. However, there are oceanic gene pools, one located in the oceans surrounding Asia and another in the Mexican Gulf. Frequent recombination means that most isolates have acquired the genetic profile of their current location. We investigated the genetic structure in the Asian gene pool by calculating the effective population size in two different ways. Under standard neutral models, the two estimates should give similar answers but we found a 27-fold difference. We propose that this discrepancy is caused by the subdivision of the species into a hundred or more ecotypes which are maintained stably in the population. To investigate the genetic factors involved, we used 51 unrelated isolates to conduct a genome-wide scan for epistatically interacting loci. We found a single example of strong epistasis between distant genome regions. A majority of strains had a type VI secretion system associated with bacterial killing. The remaining strains had genes associated with biofilm formation and regulated by cyclic dimeric GMP signaling. All strains had one or other of the two systems and none of isolate had complete complements of both systems, although several strains had remnants. Further "top down" analysis of patterns of linkage disequilibrium within frequently recombining species will allow a detailed understanding of how selection acts to structure the pattern of variation within natural bacterial populations.

  2. Mis-Diagnosis and Dual Diagnosis of Gifted Children: Gifted and LD, ADHD, OCD, Oppositional Defiant Disorder.

    ERIC Educational Resources Information Center

    Webb, James T.

    Many gifted and talented children are often misdiagnosed by psychologists and other health professionals as having Attention Deficit Hyperactivity Disorder (ADHD), Oppositional Disorder (OD), Obsessive Compulsive Disorder (OCD), or another of the mood disorders. The misdiagnosis can stem from mistaken assumptions being made about the social and…

  3. Exploring the Diagnosis of "Gifted/LD": Characterizing Postsecondary Students with Learning Disability Diagnoses at Different IQ Levels

    ERIC Educational Resources Information Center

    Lovett, Benjamin J.; Sparks, Richard L.

    2010-01-01

    Increasing numbers of students are being diagnosed as simultaneously gifted and having a learning disability, although the identification procedures and characteristics of these students are matters of continuing debate. In the present study, postsecondary students with learning disability diagnoses (N = 357) were grouped according to their IQ…

  4. Reconstruction and anticipatory Langenskiöld procedure in traumatic defect of tibial medial malleolus with type 6 physeal fracture.

    PubMed

    Abbo, Olivier; Accadbled, Frank; Laffosse, Jean-Michel; De Gauzy, Jérome Sales

    2012-09-01

    Traumatic osteoarticular or ligament defect of the tibial medial malleolus is a rare entity in children. Associated lesions may include soft tissue and joint defect, subsequent instability of the ankle, and growth arrest. We report here, the case of an 11-year-old boy, a victim of a severe trauma to the ankle, managed by an original technique. It combined a reconstruction by a composite iliac crest and gluteal fascia graft, an anticipatory Langenskiold procedure, and a serratus anterior muscle flap. This original technique proved to be a suitable alternative in this type of trauma.

  5. Comparison of brain mitochondrial cytochrome c oxidase activity with cyanide LD(50) yields insight into the efficacy of prophylactics.

    PubMed

    Marziaz, Mandy L; Frazier, Kathryn; Guidry, Paul B; Ruiz, Robyn A; Petrikovics, Ilona; Haines, Donovan C

    2013-01-01

    Cyanide inhibits cytochrome c oxidase, the terminal oxidase of the mitochondrial respiratory pathway, therefore inhibiting the cell oxygen utilization and resulting in the condition of histotoxic anoxia. The enzyme rhodanese detoxifies cyanide by utilizing sulfur donors to convert cyanide to thiocyanate, and new and improved sulfur donors are actively sought as researchers seek to improve cyanide prophylactics. We have determined brain cytochrome c oxidase activity as a marker for cyanide exposure for mice pre-treated with various cyanide poisoning prophylactics, including sulfur donors thiosulfate (TS) and thiotaurine (TT3). Brain mitochondria were isolated by differential centrifugation, the outer mitochondrial membrane was disrupted by a maltoside detergent, and the decrease in absorbance at 550 nm as horse heart ferrocytochrome c (generated by the dithiothreitol reduction of ferricytochrome c) was oxidized was monitored. Overall, the TS control prophylactic treatment provided significant protection of the cytochrome c oxidase activity. The TT3-treated mice showed reduced cytochrome c oxidase activity even in the absence of cyanide. In both treatment series, addition of exogenous Rh did not significantly enhance the prevention of cytochrome c oxidase inhibition, but the addition of sodium nitrite did. These findings can lead to a better understanding of the protection mechanism by various cyanide antidotal systems.

  6. Season of Birth Is Related to Child Retention Rates, Achievement, and Rate of Diagnosis of Specific LD

    ERIC Educational Resources Information Center

    Martin, Roy P.; Foels, Patricia; Clanton, Greg; Moon, Kathryn

    2004-01-01

    A sizable literature has demonstrated that the achievement of children in early elementary school is related to their season of birth: Those born in summer typically perform less well than those born in the fall. A small literature indicates that more children diagnosed with specific learning disabilities (SLD) are born in the summer. We have…

  7. The Perils of Multi-Lingual Students: "I'm Not LD, I'm L2 or L3."

    ERIC Educational Resources Information Center

    Kim, Kioh; Helphenstine, Derrick T.

    2017-01-01

    As more companies and families expand to the global market an increasing number of students are entering international schools outside of their home countries. Each international school is governed and run according to their own policies, but one overarching element remains: the language of instruction is usually English. When English Language…

  8. Evaluation of p-phenylenediamine, o-phenylphenol sodium salt, and 2,4-diaminotoluene in the rat comet assay as part of the Japanese Center for the Validation of Alternative Methods (JaCVAM)-initiated international validation study of in vivo rat alkaline comet assay.

    PubMed

    De Boeck, Marlies; van der Leede, Bas-jan; De Vlieger, Kathleen; Geys, Helena; Vynckier, An; Van Gompel, Jacky

    2015-07-01

    As part of the Japanese Center for the Validation of Alternative Methods (JaCVAM)-initiated international validation study of in vivo rat alkaline comet assay (comet assay), p-phenylenediamine dihydrochloride (PPD), o-phenylphenol sodium salt (OPP), and 2,4-diaminotoluene (2,4-DAT), were analyzed in this laboratory as coded test chemicals. Male Sprague-Dawley rats (7-9 weeks of age) were given three oral doses of the test compounds, 24 and 21 h apart and liver and stomach were sampled 3h after the final dose administration. Under the conditions of the test, no increases in DNA damage were observed in liver and stomach with PPD and OPP up to 100 and 1000 mg/kg/day, respectively. 2,4-DAT, a known genotoxic carcinogen, induced a weak but reproducible, dose-related and statistically significant increase in DNA damage in liver cells while no increases were observed in stomach cells.

  9. Identification of a New Type of Babesia Species in Wild Rats (Bandicota indica) in Chiang Mai Province, Thailand

    PubMed Central

    Dantrakool, Anchalee; Somboon, Pradya; Hashimoto, Tetsuo; Saito-Ito, Atsuko

    2004-01-01

    A new type of rodent babesia, which resembled Babesia microti but was phylogenetically placed closest, with the highest level of statistical support, to Babesia canis, a canine babesia, was identified in Thai Bandicota indica in Thai provinces to which malaria is endemic. Close watch should be kept on human babesiosis in Thailand. PMID:14766871

  10. Holocene malacofauna in calcareous deposits of Dūkšta site near Maišiagala in Lithuania

    NASA Astrophysics Data System (ADS)

    Sanko, Aleksander; Gaigalas, Algirdas; Rudnickaitė, Eugenija; Melešytė, Monika

    2008-01-01

    The paper contains results of new investigations in Lithuania of the first occurrences of terrestrial molluscs in calcareous tufa (travertine), carbonate content and radiocarbon dating. Terrestrial fossil molluscs (35 taxa) prevail in the mollusc fauna, while freshwater species are much more rare (6 taxa). The Dūkšta terrestrial mollusc fauna embraces a complex of thermophiles including such forest species as Acicula polita (Hartmann), Acanthinula aculeata (Müller), Aegopinella cf. pura (Alder), Bulgaria cana (Held) and Discus cf. rotundatus (Müller), as well as mainly South European Carychium tridentatum (Risso) and mainly West European Vertigo moulinsiana (Dupuy). The radiocarbon age of the sediments was found to correspond to the end of the Atlantic climatic period and the beginning of the Subboreal.

  11. Laboratory Study of Quaternary Sediment Resistivity Related to Groundwater Contamination at Mae-Hia Landfill, Mueang District, Chiang Mai Province

    NASA Astrophysics Data System (ADS)

    Sichan, N.

    2007-12-01

    This study was aimed to understand the nature of the resistivity value of the sediment when it is contaminated, in order to use the information solving the obscure interpretation in the field. The pilot laboratory experiments were designed to simulate various degree of contamination and degree of saturation then observe the resulting changes in resistivity. The study was expected to get a better understanding of how various physical parameters effect the resistivity values in term of mathematic function. And also expected to apply those obtained function to a practical quantitatively interpretation. The sediment underlying the Mae-Hia Landfill consists of clay-rich material, with interfingerings of colluvium and sandy alluvium. A systematic study identified four kinds of sediment, sand, clayey sand, sandy clay, and clay. Representative sediment and leachate samples were taken from the field and returned to the laboratory. Both the physical and chemical properties of the sediments and leachate were analyzed to delineate the necessary parameters that could be used in Archie's equation. Sediment samples were mixed with various concentration of leachate solutions. Then the resistivity values were measured at various controlled steps in the saturation degree in a well- calibrated six-electrode model resistivity box. The measured resistivity values for sand, clayey sand, sandy clay when fully and partly saturated were collected, then plotted and fitted to Archie's equation, to obtain a mathematical relationship between bulk resistivity, porosity, saturation degree and resistivity of pore fluid. The results fit well to Archie's equation, and it was possible to determine all the unknown parameters representative of the sediment samples. For sand, clayey sand, sandy clay, and clay, the formation resistivity factors (F) are 2.90, 5.77, 7.85, and 7.85 with the products of cementation factor (m) and the pore geometry factors (a) (in term of -am) are 1.49, -1.63, -1.92, -2.24 and the saturation exponents (n) are 2.06, 2.58, 3.52, and 2.46, respectively. These results were used to reinterpret the existing resistivity data of this area. The result of this reinterpretation is a map showing the quantitative distribution of the contaminant plume in the vicinity of the Mae-Hia Landfill.

  12. Results of the International Validation of the in vivo rodent alkaline comet assay for the detection of genotoxic carcinogens: Individual data for 1,2-dibromoethane, p-anisidine, and o-anthranilic acid in the 2nd step of the 4th phase Validation Study under the JaCVAM initiative.

    PubMed

    Takasawa, Hironao; Takashima, Rie; Narumi, Kazunori; Kawasako, Kazufumi; Hattori, Akiko; Kawabata, Masayoshi; Hamada, Shuichi

    2015-07-01

    As part of the Japanese Center for the Validation of Alternative Methods (JaCVAM)-initiative International Validation Study of an in vivo rat alkaline comet assay, we examined 1,2-dibromoethane (DBE), p-anisidine (ASD), and o-anthranilic acid (ANT) to investigate the effectiveness of the comet assay in detecting genotoxic carcinogens. Each of the three test chemicals was administered to 5 male Sprague-Dawley rats per group by oral gavage at 48, 24, and 3h before specimen preparation. Single cells were collected from the liver and glandular stomach at 3h after the final dosing, and the specimens prepared from these two organs were subjected to electrophoresis under alkaline conditions (pH>13). The percentage of DNA intensity in the comet tail was then assessed using an image analysis system. A micronucleus (MN) assay was also conducted using these three test chemicals with the bone marrow (BM) cells collected from the same animals simultaneously used in the comet assay, i.e., combination study of the comet assay and BM MN assay. A genotoxic (Ames positive) rodent carcinogen, DBE gave a positive result in the comet assay in the present study, while a genotoxic (Ames positive) non-carcinogen, ASD and a non-genotoxic (Ames negative) non-carcinogen, ANT showed negative results in the comet assay. All three chemicals produced negative results in the BM MN assay. While the comet assay findings in the present study were consistent with those obtained from the rodent carcinogenicity studies for the three test chemicals, we consider the positive result in the comet assay for DBE to be particularly meaningful, given that this chemical produced a negative result in the BM MN assay. Therefore, the combination study of the comet assay and BM MN assay is a useful method to detect genotoxic carcinogens that are undetectable with the BM MN assay alone.

  13. ANALYSIS OF BENZO(A)PYRENE-INDUCED DNA ADDUCTS IN MCF-7 BREAST CANCER CELLS WITH DIFFERENT LEVELS OF HSP70 EXPRESSION

    EPA Science Inventory

    Analysis of benzo[a]pyrene-induced DNA adducts in MCF-7 breast cancer cells with
    different levels of HSP7O expression.
    L.C. King1, L.D. Adams1, E.Winkfield1, J.A. Barnes2, S.D. Hester1 and J.W. Allen1. 1US
    Environmental Protection Agency, Research Triangle Park, NC 2771...

  14. Map showing bottom topography of the Pacific Continental Margin, Cape Mendocino to Point Conception

    USGS Publications Warehouse

    Chase, T.E.; Wilde, Pat; Normark, W.R.; Evenden, G.I.; Miller, C.P.; Seekins, B.A.; Young, J. D.; Grim, M.S.; Lief, C.J.

    1992-01-01

    Wilde, Pat, Chase, T.E., Holmes, M.L., Normark, W.R., Thomas, J.A., McCulloch, D.S., and Kulm, L.D., 1978, Oceanographic data off northern California-southern Oregon 40° to 43° North including the Gorda Deep Sea Fan: Berkeley, University of California, Lawrence Berkeley Laboratory Publication 251, scale 1:815,482 at 42° latitude.

  15. Map showing bottom topography of the Pacific Continental Margin, Strait of Juan de Fuca to Cape Mendocino

    USGS Publications Warehouse

    Grim, M.S.; Chase, T.E.; Evenden, G.I.; Holmes, M.L.; Normark, W.R.; Wilde, Pat; Fox, C.J.; Lief, C.J.; Seekins, B.A.

    1992-01-01

    Wilde, Pat, Chase, T.E., Holmes, M.L., Normark, W.R., Thomas, J.A., McCulloch, D.S., and Kulm, L.D., 1978, Oceanographic data off northern California-southern Oregon 40° to 43° North including the Gorda Deep Sea Fan: Berkeley, University of California, Lawrence Berkeley Laboratory Publication 251, scale 1:815,482 at 42° latitude.

  16. Adaptive management for ecosystem services (j/a) | Science ...

    EPA Pesticide Factsheets

    Management of natural resources for the production of ecosystem services, which are vital for human well-being, is necessary even when there is uncertainty regarding system response to management action. This uncertainty is the result of incomplete controllability, complex internal feedbacks, and non-linearity that often interferes with desired management outcomes, and insufficient understanding of nature and people. Adaptive management was developed to reduce such uncertainty. We present a framework for the application of adaptive management for ecosystem services that explicitly accounts for cross-scale tradeoffs in the production of ecosystem services. Our framework focuses on identifying key spatiotemporal scales (plot, patch, ecosystem, landscape, and region) that encompass dominant structures and processes in the system, and includes within- and cross-scale dynamics, ecosystem service tradeoffs, and management controllability within and across scales. Resilience theory recognizes that a limited set of ecological processes in a given system regulate ecosystem services, yet our understanding of these processes is poorly understood. If management actions erode or remove these processes, the system may shift into an alternative state unlikely to support the production of desired services. Adaptive management provides a process to assess the underlying within and cross-scale tradeoffs associated with production of ecosystem services while proceeding with manage

  17. Ecology for the shrinking city (JA) | Science Inventory | US ...

    EPA Pesticide Factsheets

    This article brings together the concepts of shrinking cities—the hundreds of cities worldwide experiencing long-term population loss—and ecology for the city. Ecology for the city is the application of a social–ecological understanding to shaping urban form and function along sustainable trajectories. Ecology for the shrinking city therefore acknowledges that urban transformations to sustainable trajectories may be quite different in shrinking cities as compared with growing cities. Shrinking cities are well poised for transformations, because shrinking is perceived as a crisis and can mobilize the social capacity to change. Ecology is particularly well suited to contribute solutions because of the extent of vacant land in shrinking cities that can be leveraged for ecosystem-services provisioning. A crucial role of an ecology for the shrinking city is identifying innovative pathways that create locally desired amenities that provide ecosystem services and contribute to urban sustainability at multiple scales. This paper brings together the concepts of ecology for the city and shrinking cities – the hundreds of cities worldwide experiencing long-term population loss. Ecology for the city is the application of social-ecological understanding to shaping urban form and function along sustainable trajectories. Ecology for the shrinking city acknowledges that urban transformations to sustainable trajectories may be quite different in shrinking cities as compa

  18. Adaptive management for ecosystem services (j/a)

    EPA Science Inventory

    Management of natural resources for the production of ecosystem services, which are vital for human well-being, is necessary even when there is uncertainty regarding system response to management action. This uncertainty is the result of incomplete controllability, complex intern...

  19. Treatment Plan Adherence for Your Child With JA

    MedlinePlus

    ... Story into a Message of Hope Margo Deihl: Music is Medicine Liz Morasso: Flying High Brett Ishihara: ... Story into a Message of Hope Margo Deihl: Music is Medicine Liz Morasso: Flying High Treatment Plan ...

  20. Learning Disabilities Spectrum: ADD, ADHD, and LD. Proceedings of the Annual Spectrum of Developmental Disabilities Course (15th, Baltimore, Maryland, March 16-18, 1992).

    ERIC Educational Resources Information Center

    Capute, Arnold J., Ed.; And Others

    This volume, the proceedings of a 1992 conference, presents 14 papers which take a neuropsychological approach to attention deficit disorder, attention deficit disorder with hyperactivity, and learning disabilities. Papers have the following titles and authors: "A New Conceptual Model for Dyslexia" (Sally E. Shaywitz et al.);…