Science.gov

Sample records for ja selle omadustele

  1. Selling Languages

    ERIC Educational Resources Information Center

    Varela-Ibarra, Jose L.

    1975-01-01

    To reverse trends toward reductions in the number of foreign language teaching positions, it is necessary to change the negative image associated with foreign languages and to try to attract more students. A four-point selling program is suggested. (Author/RM)

  2. Selling Your Credits.

    ERIC Educational Resources Information Center

    Steiner, Dawn; Murtagh, Maria

    1982-01-01

    A Credits-For-Resale program underwritten by the California Association of College Stores is described. A brief history of the program, guidelines on the mechanics involved, and suggestions on how selling credits could work for individual stores are presented. (MLW)

  3. To Sell An Idea.

    ERIC Educational Resources Information Center

    Stone, Alan J.

    1986-01-01

    The experiences of Aurora University, a small university that not only raised money but established new links with donors, are described. The key to fund raising is selling an idea. As donors become more sophisticated, sentiment and traditional patterns play a less significant role in motivating donors. (MLW)

  4. SELL — EDRN Public Portal

    Cancer.gov

    From NCBI Gene and UniProtKB/Swiss-Prot: sL-selectin, also known as SELL, is a cell surface adhesion molecule that belongs to a family of adhesion/homing receptors. SELL mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes and promotes initial tethering and rolling of leukocytes in endothelia, facilitating their migration into secondary lymphoid organs and inflammation sites. SELL contains a C-type lectin-like domain, a calcium-binding epidermal growth factor-like domain, and two short complement-like repeats. Alternatively spliced transcript variants have been found for this gene.

  5. Show and Sell: Teaching Sales through Hands-On Selling

    ERIC Educational Resources Information Center

    Rippé, Cindy B.

    2015-01-01

    There is a shortage of qualified salespeople, which creates a challenge for educators to prepare more students for a sales career. One of the most common teaching techniques used in preparing students is role playing, which mirrors real-world selling. However, role plays are not necessarily authentic because the players and conditions are not a…

  6. The Selling of the Sheepskin.

    ERIC Educational Resources Information Center

    Mackey, Maureen

    1980-01-01

    As higher education turns from a seller's to a buyer's market, colleges are using marketing strategy as an aid for student recruitment. Unethical and ethical promotion and recruiting practices, recruiting abuses (selling of immigration papers, etc.), legal contractual responsibilities, ethical decay, and consumer rights of students are discussed.…

  7. Advertising: To Get The Reader to Buy, Buy, Buy, You Must Sell, Sell, Sell.

    ERIC Educational Resources Information Center

    Melton, Rob

    1998-01-01

    Offers an overview of advertising as it relates to student publications. Discusses what advertising is; what the consumer wants; the buyer and seller; creating a selling strategy; basic building blocks; advertising art; and text. Describes basic guidelines for designing an ad, outlines some assignments for students, and notes a few things not to…

  8. 13 CFR 120.951 - Selling agent.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Selling agent. 120.951 Section 120.951 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION BUSINESS LOANS Development Company Loan Program (504) Debenture Sales and Service Agents § 120.951 Selling agent. The CDC, with...

  9. 13 CFR 120.951 - Selling agent.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 13 Business Credit and Assistance 1 2012-01-01 2012-01-01 false Selling agent. 120.951 Section 120.951 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION BUSINESS LOANS Development Company Loan Program (504) Debenture Sales and Service Agents § 120.951 Selling agent. The CDC, with...

  10. 13 CFR 120.951 - Selling agent.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 13 Business Credit and Assistance 1 2013-01-01 2013-01-01 false Selling agent. 120.951 Section 120.951 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION BUSINESS LOANS Development Company Loan Program (504) Debenture Sales and Service Agents § 120.951 Selling agent. The CDC, with...

  11. 13 CFR 120.951 - Selling agent.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 13 Business Credit and Assistance 1 2011-01-01 2011-01-01 false Selling agent. 120.951 Section 120.951 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION BUSINESS LOANS Development Company Loan Program (504) Debenture Sales and Service Agents § 120.951 Selling agent. The CDC, with...

  12. 13 CFR 120.951 - Selling agent.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 13 Business Credit and Assistance 1 2014-01-01 2014-01-01 false Selling agent. 120.951 Section 120.951 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION BUSINESS LOANS Development Company Loan Program (504) Debenture Sales and Service Agents § 120.951 Selling agent. The CDC, with...

  13. The Advanced Course in Professional Selling

    ERIC Educational Resources Information Center

    Loe, Terry; Inks, Scott

    2014-01-01

    More universities are incorporating sales content into their curriculums, and although the introductory courses in professional sales have much common ground and guidance from numerous professional selling texts, instructors teaching the advanced selling course lack the guidance provided by common academic tools and materials. The resulting…

  14. Selling to the moneyed masses.

    PubMed

    Nunes, Paul F; Johnson, Brian A; Breene, R Timothy S

    2004-01-01

    Over the past decade, the distribution of household incomes has shifted so much that a much larger proportion of consumers now earn significantly higher-than-average incomes--while still falling short of being truly rich. As a result, what used to be a no-man's-land for new product introductions has in many categories become an extremely profitable "new middle ground." How can marketers capitalize on this new territory? The key, say the authors, is to rethink the positioning and design of offerings and the ways they can be brought to market. Take, for instance, how Procter & Gamble redefined the positioning map for tooth-whitening solutions. A decade ago, dental centers were popularizing expensive bleaching techniques that put the price of a professionally brightened smile in the 400 dollars range. At the low end, consumers also had the choice of whitening toothpastes that cost anywhere from 2 dollars to 8 dollars. P&G wisely positioned itself between the two ends, successfully targeting the new mass market with its 35 dollars Whitestrips. In product categories where it's clear the middle ground has already been populated, it's important for companies to design or redesign offerings to compete. An example is the Polo shirt. How do you sell a man yet another one after he's bought every color he wants? Add some features, and call it a golf shirt. Here, marketers have introduced designs based on the concept of "occasional use" in order to stand out. Finally, companies wishing to reach the "almost rich" can change how they go to market. Perhaps no mass retailer has made a stronger bid for the mass affluent than Target Stores, which has pioneered a focus the company itself characterizes as upscale discount. The strategy has made Target an everyday shopping phenomenon among well-heeled urbanites and prosperous professionals.

  15. 29 CFR 541.504 - Drivers who sell.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 29 Labor 3 2013-07-01 2013-07-01 false Drivers who sell. 541.504 Section 541.504 Labor Regulations... Outside Sales Employees § 541.504 Drivers who sell. (a) Drivers who deliver products and also sell such... sales. In determining the primary duty of drivers who sell, work performed incidental to and...

  16. 29 CFR 541.504 - Drivers who sell.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 29 Labor 3 2012-07-01 2012-07-01 false Drivers who sell. 541.504 Section 541.504 Labor Regulations... Outside Sales Employees § 541.504 Drivers who sell. (a) Drivers who deliver products and also sell such... sales. In determining the primary duty of drivers who sell, work performed incidental to and...

  17. 29 CFR 541.504 - Drivers who sell.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 29 Labor 3 2014-07-01 2014-07-01 false Drivers who sell. 541.504 Section 541.504 Labor Regulations... Outside Sales Employees § 541.504 Drivers who sell. (a) Drivers who deliver products and also sell such... sales. In determining the primary duty of drivers who sell, work performed incidental to and...

  18. 29 CFR 541.504 - Drivers who sell.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 29 Labor 3 2010-07-01 2010-07-01 false Drivers who sell. 541.504 Section 541.504 Labor Regulations... Outside Sales Employees § 541.504 Drivers who sell. (a) Drivers who deliver products and also sell such... sales. In determining the primary duty of drivers who sell, work performed incidental to and...

  19. 29 CFR 541.504 - Drivers who sell.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 29 Labor 3 2011-07-01 2011-07-01 false Drivers who sell. 541.504 Section 541.504 Labor Regulations... Outside Sales Employees § 541.504 Drivers who sell. (a) Drivers who deliver products and also sell such... sales. In determining the primary duty of drivers who sell, work performed incidental to and...

  20. 13 CFR 120.431 - Which Lenders may sell, sell participations in, or pledge 7(a) loans?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Which Lenders may sell, sell participations in, or pledge 7(a) loans? 120.431 Section 120.431 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION BUSINESS LOANS Lenders Other Conveyances § 120.431 Which Lenders may sell, sell participations...

  1. Reconsidering Kantian arguments against organ selling.

    PubMed

    Alpinar-Şencan, Zümrüt

    2016-03-01

    Referring to Kant's arguments addressing the moral relationship between our bodies and ourselves is quite common in contemporary debate about organ selling, although he does not provide us with any specific arguments related to this debate. It is widely argued that the most promising way to show the moral impermissibility of organ selling is to mount an argument on Kantian grounds. This paper asks whether it is possible to argue coherently against organ selling in a Kantian framework. It will be shown that by mounting the argument on Kantian grounds no compelling argument can be given against sale of organs, either because the arguments apply to donation of organs, too, or the arguments are not convincing for other independent reasons. In the first section, it will be argued that donation and selling are not distinguishable in a Kantian framework, since the concern about commodification of the body and its parts shall be raised by both actions. In the second section, some contemporary accounts inspired by Kant will be presented and discussed separately. It will be argued that the reasons for promoting organ donation while arguing against selling clash with each other in an unconvincing way.

  2. Selling to Industry for Sheltered Workshops.

    ERIC Educational Resources Information Center

    Rehabilitation Services Administration (DHEW), Washington, DC.

    Intended for staffs of sheltered workshops for handicapped individuals, the guide presents a plan for selling the workshop idea to industry, hints on meeting obstacles, and ideas for expanding and upgrading workshop contract promotion. Brief sections cover the following topics (example subtopics are in parentheses): finding work contract prospects…

  3. Petroleum Marketing. Selling Automotive Products and Services.

    ERIC Educational Resources Information Center

    Luter, Robert R.

    This textbook contains material for the individualized instruction of students training for careers in service stations; automotive, tire, battery, and accessory retail stores; oil jobbers and petroleum product wholesalers, or any wholesale or retail establishment that sells automotive products and services. Included among the topics addressed in…

  4. Selling real estate to meet capital needs.

    PubMed

    Rosenthal, Robert A; Nelson, Gregory P

    2003-05-01

    Real estate can provide a means for hospitals to raise capital. Selling a building and investing proceeds in revenue-producing operations may yield greater return than rental income. In a ground lease, a hospital can require the buyer to adhere to certain limitations that are beneficial to the hospital's strategic goals.

  5. Advanced Selling: A Comprehensive Course Sales Project

    ERIC Educational Resources Information Center

    Yarrington-Young, Susan; Castleberry, Stephen B.; Coleman, Joshua T.

    2016-01-01

    A comprehensive project for the Advanced Selling course that has been tested at three universities is introduced. After selecting an industry and a company, students engage in a complete industry analysis, a company sales analysis, a sales-specific SWOT analysis, complete a ride day with a salesperson in that firm, then present their findings in a…

  6. Teaching Written Communication Skills in Professional Selling: The Cover Letter

    ERIC Educational Resources Information Center

    West, Vicki L.

    2006-01-01

    The selling process steps have been an integral part of professional selling courses and textbooks for years. Although slight changes have been made in their wording and format, most textbooks are consistent in the recommended process for an effective sales interaction. In an effort to combine teaching the selling process with the increased demand…

  7. 2 CFR 200.467 - Selling and marketing costs.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 2 Grants and Agreements 1 2014-01-01 2014-01-01 false Selling and marketing costs. 200.467 Section 200.467 Grants and Agreements Office of Management and Budget Guidance for Grants and Agreements... Cost § 200.467 Selling and marketing costs. Costs of selling and marketing any products or services...

  8. An Empirical Look at Professional Selling from a Student Perspective

    ERIC Educational Resources Information Center

    Bristow, Dennis N.; Amyx, Douglas; Slack, Jennifer; Gulati, Rajesh

    2006-01-01

    In this article, the authors examined students' perceptions of professional selling. They found significant differences between the perceptions of students who had completed personal selling courses and students who had not. The authors observed differences in students' perceptions of personal selling as a career, the contribution of personal…

  9. Strategies for selling and consolidating physician practices.

    PubMed

    Mancino, D M

    1997-01-01

    The changing dynamics of healthcare service delivery is forcing many physicians to consider selling their practices to hospitals or health plans or consolidating them with other practices. Besides being subject to the corporate and tax requirements that apply to the sale of any business, the sale of physician practices is also subject to Federal fraud and abuse and self-referral laws. Several sale strategies are available to physicians who desire to sell or consolidate their practices, including asset sales, stock sales, forward mergers, drop down consolidations, spinoffs, and statutory mergers. Each strategy has advantages and disadvantages, but whichever strategy is chosen, both sellers and buyers must ensure that tax issues are addressed and that the transaction complies with the requirements of Federal anti-kickback and self-referral laws.

  10. Cocaine selling among urban black and white adolescent males.

    PubMed

    Dembo, R; Williams, L; Schmeidler, J

    1994-12-01

    Data from a longitudinal study of juvenile detainees are used to examine the relationships between cocaine selling, substance use, and other delinquency among the Black and White males in the study. A descriptive comparison of rates of cocaine selling among the youths is followed by a descriptive comparison of prevalence of substance use and other delinquency across four subgroups: White and Black males indicating they sold and did not sell cocaine. These comparisons are followed by analyses of variance examining the relationships between involvement in substance use and other delinquency, and ethnicity, cocaine selling, and the interaction of ethnicity and cocaine selling. Important ethnicity and cocaine-selling effects are found, but not ethnicity by cocaine-selling interactions. The implications of our findings for theory and service provision are drawn.

  11. Sell Energy-Efficient Products: A Guide to Selling to the U.S. Government

    SciTech Connect

    2012-12-01

    The Federal Government spends $500 billion on goods and services every year and $20 billion on energy. For many product types, the U.S. Government is the single largest purchaser. Manufacturers and vendors can increase their sales potential by helping Federal purchasers meet their energy-efficient product purchasing requirements. This guide explains how to sell products to the government.

  12. Nuts and bolts issues to consider when selling your practice.

    PubMed

    Schlager, D D; Miaoulis, G

    1995-01-01

    Ten issues are provided that will help physicians and administrators through the process of selling a practice. Those issues include determining the buyers reasons for buying, determine your reason for selling, finding the right people, how the buyer is valuing your practice, how you value your practice, preparing the selling package, employment agreements and compensation packages, negotiating the operating contract, tax consequences and knowing when to walk away from the deal.

  13. Selling eugenics: the case of Sweden.

    PubMed

    Bjorkman, Maria; Widmalm, Sven

    2010-12-20

    This paper traces the early (1910s to 1920s) development of Swedish eugenics through a study of the social network that promoted it. The eugenics network consisted mainly of academics from a variety of disciplines, but with medicine and biology dominating; connections with German scientists who would later shape Nazi biopolitics were strong. The paper shows how the network used political lobbying (for example, using contacts with academically accomplished MPs) and various media strategies to gain scientific and political support for their cause, where a major goal was the creation of a eugenics institute (which opened in 1922). It also outlines the eugenic vision of the institute's first director, Herman Lundborg. In effect the network, and in particular Lundborg, promoted the view that politics should be guided by eugenics and by a genetically superior elite. The selling of eugenics in Sweden is an example of the co-production of science and social order.

  14. 48 CFR 31.205-38 - Selling costs.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 1 2011-10-01 2011-10-01 false Selling costs. 31.205-38... CONTRACTING REQUIREMENTS CONTRACT COST PRINCIPLES AND PROCEDURES Contracts With Commercial Organizations 31.205-38 Selling costs. (a) “Selling” is a generic term encompassing all efforts to market...

  15. 48 CFR 31.205-38 - Selling costs.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... induce particular customers to purchase particular products or services of the contractor. Direct selling... customer with the contractor's products or services, conditions of sale, service capabilities, etc. It also... contractor's products or services for a particular customer's use. The cost of direct selling efforts...

  16. 32 CFR 811.4 - Selling visual information materials.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 32 National Defense 6 2010-07-01 2010-07-01 false Selling visual information materials. 811.4 Section 811.4 National Defense Department of Defense (Continued) DEPARTMENT OF THE AIR FORCE SALES AND SERVICES RELEASE, DISSEMINATION, AND SALE OF VISUAL INFORMATION MATERIALS § 811.4 Selling...

  17. 32 CFR 811.4 - Selling visual information materials.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... motion media to the DVIC. The center may sell other Air Force VI motion picture and television materials... Section 811.4 National Defense Department of Defense (Continued) DEPARTMENT OF THE AIR FORCE SALES AND... information materials. (a) Air Force VI activities cannot sell materials. (b) HQ AFCIC/ITSM may approve...

  18. 32 CFR 811.4 - Selling visual information materials.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... motion media to the DVIC. The center may sell other Air Force VI motion picture and television materials... Section 811.4 National Defense Department of Defense (Continued) DEPARTMENT OF THE AIR FORCE SALES AND... information materials. (a) Air Force VI activities cannot sell materials. (b) HQ AFCIC/ITSM may approve...

  19. 47 CFR 73.4005 - Advertising-refusal to sell.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 47 Telecommunication 4 2011-10-01 2011-10-01 false Advertising-refusal to sell. 73.4005 Section 73.4005 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) BROADCAST RADIO SERVICES RADIO BROADCAST SERVICES Rules Applicable to All Broadcast Stations § 73.4005 Advertising—refusal to sell. See...

  20. 47 CFR 73.4005 - Advertising-refusal to sell.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 47 Telecommunication 4 2010-10-01 2010-10-01 false Advertising-refusal to sell. 73.4005 Section 73.4005 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) BROADCAST RADIO SERVICES RADIO BROADCAST SERVICES Rules Applicable to All Broadcast Stations § 73.4005 Advertising—refusal to sell. See...

  1. 47 CFR 73.4005 - Advertising-refusal to sell.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 47 Telecommunication 4 2013-10-01 2013-10-01 false Advertising-refusal to sell. 73.4005 Section 73.4005 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) BROADCAST RADIO SERVICES RADIO BROADCAST SERVICES Rules Applicable to All Broadcast Stations § 73.4005 Advertising—refusal to sell. See...

  2. 47 CFR 73.4005 - Advertising-refusal to sell.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 47 Telecommunication 4 2014-10-01 2014-10-01 false Advertising-refusal to sell. 73.4005 Section 73.4005 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) BROADCAST RADIO SERVICES RADIO BROADCAST SERVICES Rules Applicable to All Broadcast Stations § 73.4005 Advertising—refusal to sell. See...

  3. 47 CFR 73.4005 - Advertising-refusal to sell.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 47 Telecommunication 4 2012-10-01 2012-10-01 false Advertising-refusal to sell. 73.4005 Section 73.4005 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) BROADCAST RADIO SERVICES RADIO BROADCAST SERVICES Rules Applicable to All Broadcast Stations § 73.4005 Advertising—refusal to sell. See...

  4. A Consumer-Driven Approach To Increase Suggestive Selling.

    ERIC Educational Resources Information Center

    Rohn, Don; Austin, John; Sanford, Alison

    2003-01-01

    Discussion of the effectiveness of behavioral interventions in improving suggestive selling behavior of sales staff focuses on a study that examined the efficacy of a consumer-driven approach to improve suggestive selling behavior of three employees of a fast food franchise. Reports that consumer-driven intervention increased suggestive selling…

  5. 18 CFR 292.313 - Reinstatement of obligation to sell.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... finding under § 292.312 relieving an electric utility of its obligation to sell electric energy, a... purchase electric energy under this section. Such application shall set forth the factual basis upon which... application reinstating the electric utility's obligation to sell electric energy under this section if...

  6. 18 CFR 292.313 - Reinstatement of obligation to sell.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... finding under § 292.312 relieving an electric utility of its obligation to sell electric energy, a... purchase electric energy under this section. Such application shall set forth the factual basis upon which... application reinstating the electric utility's obligation to sell electric energy under this section if...

  7. 18 CFR 292.313 - Reinstatement of obligation to sell.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... finding under § 292.312 relieving an electric utility of its obligation to sell electric energy, a... purchase electric energy under this section. Such application shall set forth the factual basis upon which... application reinstating the electric utility's obligation to sell electric energy under this section if...

  8. 18 CFR 292.313 - Reinstatement of obligation to sell.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... finding under § 292.312 relieving an electric utility of its obligation to sell electric energy, a... purchase electric energy under this section. Such application shall set forth the factual basis upon which... application reinstating the electric utility's obligation to sell electric energy under this section if...

  9. 18 CFR 292.313 - Reinstatement of obligation to sell.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... finding under § 292.312 relieving an electric utility of its obligation to sell electric energy, a... purchase electric energy under this section. Such application shall set forth the factual basis upon which... application reinstating the electric utility's obligation to sell electric energy under this section if...

  10. 36 CFR 223.1 - Authority to sell timber.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... General Provisions § 223.1 Authority to sell timber. Trees, portions of trees, and other forest products... 36 Parks, Forests, and Public Property 2 2013-07-01 2013-07-01 false Authority to sell timber. 223.1 Section 223.1 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE...

  11. 36 CFR 223.1 - Authority to sell timber.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... General Provisions § 223.1 Authority to sell timber. Trees, portions of trees, and other forest products... 36 Parks, Forests, and Public Property 2 2011-07-01 2011-07-01 false Authority to sell timber. 223.1 Section 223.1 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE...

  12. 36 CFR 223.1 - Authority to sell timber.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... General Provisions § 223.1 Authority to sell timber. Trees, portions of trees, and other forest products... 36 Parks, Forests, and Public Property 2 2014-07-01 2014-07-01 false Authority to sell timber. 223.1 Section 223.1 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE...

  13. 36 CFR 223.1 - Authority to sell timber.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... General Provisions § 223.1 Authority to sell timber. Trees, portions of trees, and other forest products... 36 Parks, Forests, and Public Property 2 2012-07-01 2012-07-01 false Authority to sell timber. 223.1 Section 223.1 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE...

  14. Adolescents' Lifetime Experience of Selling Sex: Development over Five Years

    ERIC Educational Resources Information Center

    Fredlund, Cecilia; Svensson, Frida; Svedin, Carl Goran; Priebe, Gisela; Wadsby, Marie

    2013-01-01

    Lifetime experience of selling sex among adolescents was investigated together with sociodemographic correlates, parent-child relationship, and the existence of people to confide in. Changes over time regarding the selling of sex were investigated through a comparison of data from 2004 and 2009. This study was carried out using 3,498 adolescents…

  15. Suggestions for Successfully Establishing a University Selling Center

    ERIC Educational Resources Information Center

    Shepherd, C. David; Eastman, Jacqueline K.

    2008-01-01

    The authors describe the multiple benefits a university selling center offers to students, faculty members, administrators, and the general business community. The seven essential steps in first establishing a university selling center are addressed: find a champion, obtain the support of administration, find a white knight, establish a board of…

  16. A Study On Direct Selling Through Multi Level Marketing

    NASA Astrophysics Data System (ADS)

    Merlin, F. Mary

    2012-09-01

    Direct selling is a multi-level marketing in which the sales force is compensated not only for the sales they make but also for the sales done through their recruit. This recruited sales force is referred to as the participants who can provide multiple levels of compensation.A person's job would be to recruit others to sell their product, and in return, receive a percentage of their sales. The next person's job then is to recruit people even more so below them, and receive a percentage of their sales. Other terms for Multi-level marketing include network marketing and referral marketing. Commonly, the salespeople are expected to sell products directly to consumers by means of relationship through referrals marketing. Some people use direct selling as a synonym for MLM, although MLM is only one type of direct selling

  17. Learning to Sell Door to Door: Teaching as Persuasion

    ERIC Educational Resources Information Center

    Bogdan, Robert

    1972-01-01

    Two national firms with standardized marketing schemes employ persuasion rather than instruction in teaching an employee to sell. Students leave for their first assignment convinced of success in spite of a lack of skill. (JB)

  18. Evaluation of Conceptual Selling as a job development planning process.

    PubMed

    Carlson, Linda; Smith, Galen; Rapp, Charles A

    2008-01-01

    Job development is integral to evidence-based supported employment practice. This paper reviews the limited research on job development and critically examines various definitions of job development. The authors describe Conceptual Selling, an existing method currently used in sales that can be adopted for use in job development. An exploratory evaluation of Conceptual Selling as a method for job development is described and implications discussed.

  19. Gang Membership, Drug Selling, and Violence in Neighborhood Context.

    PubMed

    Bellair, Paul E; McNulty, Thomas L

    2009-12-01

    A prominent perspective in the gang literature suggests that gang member involvement in drug selling does not necessarily increase violent behavior. In addition it is unclear from previous research whether neighborhood disadvantage strengthens that relationship. We address those issues by testing hypotheses regarding the confluence of neighborhood disadvantage, gang membership, drug selling, and violent behavior. A three-level hierarchical model is estimated from the first five waves of the 1997 National Longitudinal Survey of Youth, matched with block-group characteristics from the 2000 U.S. Census. Results indicate that (1) gang members who sell drugs are significantly more violent than gang members that don't sell drugs and drug sellers that don't belong to gangs; (2) drug sellers that don't belong to gangs and gang members who don't sell drugs engage in comparable levels of violence; and (3) an increase in neighborhood disadvantaged intensifies the effect of gang membership on violence, especially among gang members that sell drugs.

  20. Young women selling sex online – narratives on regulating feelings

    PubMed Central

    Jonsson, Linda S; Svedin, Carl Göran; Hydén, Margareta

    2015-01-01

    The current study concerns young women’s life stories of their experiences selling sex online before the age of 18. The aim was to gain an understanding of young women’s perceptions of the reasons they started, continued, and stopped selling sex. The study included interviews with 15 young women between the ages of 15 and 25 (M=18.9). Thematic analysis was used to identify similarities and differences in the narratives. Three themes and eight sub-themes were identified in relation to different stages in their lives in the sex trade. The themes were organized into three parts, each with its own storyline: “Entering – adverse life experiences”; traumatic events: feeling different and being excluded. “Immersion – using the body as a tool for regulating feelings”; being seen: being touched: being in control: affect regulation and self-harming. “Exiting – change or die”; living close to death: the process of quitting. The informants all had stable social lives in the sense that they had roofs over their heads, food to eat, and no substance-abuse issues. None had a third party who arranged the sexual contacts and none were currently trafficked. They described how their experiences of traumatic events and of feeling different and excluded had led them into the sex trade. Selling sex functioned as a way to be seen, to handle traumatic events, and to regulate feelings. Professionals working with young people who sell sex online need to understand the complex web of mixed feelings and emotional needs that can play a role in selling sex. Young people selling sex might need guidance in relationship building as well as help processing traumatic experiences and ending self-harming behavior. Further studies are needed on the functions of online sex selling and on the exit process for young people, in order to prevent entrance and facilitate exiting. PMID:25733944

  1. Young women selling sex online - narratives on regulating feelings.

    PubMed

    Jonsson, Linda S; Svedin, Carl Göran; Hydén, Margareta

    2015-01-01

    The current study concerns young women's life stories of their experiences selling sex online before the age of 18. The aim was to gain an understanding of young women's perceptions of the reasons they started, continued, and stopped selling sex. The study included interviews with 15 young women between the ages of 15 and 25 (M=18.9). Thematic analysis was used to identify similarities and differences in the narratives. Three themes and eight sub-themes were identified in relation to different stages in their lives in the sex trade. The themes were organized into three parts, each with its own storyline: "Entering - adverse life experiences"; traumatic events: feeling different and being excluded. "Immersion - using the body as a tool for regulating feelings"; being seen: being touched: being in control: affect regulation and self-harming. "Exiting - change or die"; living close to death: the process of quitting. The informants all had stable social lives in the sense that they had roofs over their heads, food to eat, and no substance-abuse issues. None had a third party who arranged the sexual contacts and none were currently trafficked. They described how their experiences of traumatic events and of feeling different and excluded had led them into the sex trade. Selling sex functioned as a way to be seen, to handle traumatic events, and to regulate feelings. Professionals working with young people who sell sex online need to understand the complex web of mixed feelings and emotional needs that can play a role in selling sex. Young people selling sex might need guidance in relationship building as well as help processing traumatic experiences and ending self-harming behavior. Further studies are needed on the functions of online sex selling and on the exit process for young people, in order to prevent entrance and facilitate exiting.

  2. Availability of websites offering to sell psilocybin spores and psilocybin.

    PubMed

    Lott, Jason P; Marlowe, Douglas B; Forman, Robert F

    2009-09-01

    This study assesses the availability of websites offering to sell psilocybin spores and psilocybin, a powerful hallucinogen contained in Psilocybe mushrooms. Over a 25-month period beginning in March 2003, eight searches were conducted in Google using the term "psilocybin spores." In each search the first 100 nonsponsored links obtained were scored by two independent raters according to standardized criteria to determine whether they offered to sell psilocybin or psilocybin spores. No attempts were made to procure the products offered for sale in order to ascertain whether the marketed psilocybin was in fact "genuine" or "counterfeit." Of the 800 links examined, 58% led to websites offering to sell psilocybin spores. Additionally, evidence that whole Psilocybe mushrooms are offered for sale online was obtained. Psilocybin and psilocybin spores were found to be widely available for sale over the Internet. Online purchase of psilocybin may facilitate illicit use of this potent psychoactive substance. Additional studies are needed to assess whether websites offering to sell psilocybin and psilocybin spores actually deliver their products as advertised.

  3. A Descriptive Analysis of Best-Selling Basic Course Texts.

    ERIC Educational Resources Information Center

    Worley, David W.; Worley, Debra A.; McMahan, David

    A study examined eight best-selling hybrid texts for public speaking or speech communication courses, looking at how each text is organized, what the focus is, which topics are included, how many pages are dedicated to each topic, which pedagogical features are incorporated into each text, and what ancillaries are available with each text. The…

  4. Why HPT Will Continue to Be a Hard Sell

    ERIC Educational Resources Information Center

    Pearlstein, Richard B.

    2012-01-01

    Most executives have not heard of human performance technology (HPT), but a recent Google search showed 25 times more Google hits for "lean six sigma" than for "human performance technology." This article describes five factors that make HPT a hard sell: (1) HPT is not part of standard business jargon, (2) organizational executives associate…

  5. Our Hard Sell Almost Blew a Successful Levy Campaign.

    ERIC Educational Resources Information Center

    Daniel, George T.

    1985-01-01

    Based on his own experience, a school executive counsels against using a hard sell in promoting a bond issue, since Washington State public disclosure law requires that school levy material printed and distributed by the school system be strictly limited to factual information. (TE)

  6. Retail Florist: Selling the Floral Product, Maintenance and Delivery.

    ERIC Educational Resources Information Center

    Southern Illinois Univ., Carbondale.

    This retail florist unit guide is provided to help teachers teach units on sales of floral products and maintenance and delivery in a floral shop. Topics covered in the selling unit are basic mathematics; taxable items; sales etiquette; types of floral products; telephone etiquette; order form information; wire service regulations; care of floral…

  7. 36 CFR 223.1 - Authority to sell timber.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    .... Trees, portions of trees, and other forest products on National Forest System lands may be sold for the... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Authority to sell timber. 223.1 Section 223.1 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE...

  8. 17 CFR 229.507 - (Item 507) Selling security holders.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... holders. 229.507 Section 229.507 Commodity and Securities Exchanges SECURITIES AND EXCHANGE COMMISSION... Provisions § 229.507 (Item 507) Selling security holders. If any of the securities to be registered are to be offered for the account of security holders, name each such security holder, indicate the nature of...

  9. 17 CFR 229.507 - (Item 507) Selling security holders.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... holders. 229.507 Section 229.507 Commodity and Securities Exchanges SECURITIES AND EXCHANGE COMMISSION... Provisions § 229.507 (Item 507) Selling security holders. If any of the securities to be registered are to be offered for the account of security holders, name each such security holder, indicate the nature of...

  10. 17 CFR 229.507 - (Item 507) Selling security holders.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... holders. 229.507 Section 229.507 Commodity and Securities Exchanges SECURITIES AND EXCHANGE COMMISSION... Provisions § 229.507 (Item 507) Selling security holders. If any of the securities to be registered are to be offered for the account of security holders, name each such security holder, indicate the nature of...

  11. 17 CFR 229.507 - (Item 507) Selling security holders.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... holders. 229.507 Section 229.507 Commodity and Securities Exchanges SECURITIES AND EXCHANGE COMMISSION... Provisions § 229.507 (Item 507) Selling security holders. If any of the securities to be registered are to be offered for the account of security holders, name each such security holder, indicate the nature of...

  12. 17 CFR 229.507 - (Item 507) Selling security holders.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... holders. 229.507 Section 229.507 Commodity and Securities Exchanges SECURITIES AND EXCHANGE COMMISSION... Provisions § 229.507 (Item 507) Selling security holders. If any of the securities to be registered are to be offered for the account of security holders, name each such security holder, indicate the nature of...

  13. Do Young Children Understand the Selling Intent of Commercials?

    ERIC Educational Resources Information Center

    Macklin, M. Carole

    1985-01-01

    In a study that included nonverbal measures, young children indicated little understanding of the selling intent of commercials. Researchers interested in advertising effects on children are urged to consider the necessity and desirability of improved nonverbal measures in dealing with a subject population with limited language facility.…

  14. Wholesale Selling, a Distributive Education Manual and Answer Book.

    ERIC Educational Resources Information Center

    Batis, Harry P.

    This document containing assignments on 16 varied topics, with objectives, content information, and a separate answer book was designed to be used by secondary or post-secondary distributive education students and by wholesale distributors for use as training guides for employees. Topics discussed include: (1) thinking about selling today, (2)…

  15. Commercialism in Schools: Supporting Students or Selling Access?

    ERIC Educational Resources Information Center

    Robelen, Erik W.

    1998-01-01

    This information brief discusses the impact of commercialism in schools. It asks the question of whether such advertising is supporting students or is simply selling access. It describes how children are a desirable market since they have most of their purchases ahead of them; they can also frequently convince parents to buy items. The brief…

  16. Service Learning Inputs and Outcomes in a Personal Selling Course

    ERIC Educational Resources Information Center

    Hagenbuch, David J.

    2006-01-01

    To improve the use of service learning in the marketing curriculum, Petkus (2000) recommended that future research focus on empirical studies of service learning in specific marketing courses. Personal selling represents a key component of marketing that is quite amenable to service learning, yet very little research has examined the use of…

  17. 29 CFR 780.710 - A country elevator may sell products and services to farmers.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 29 Labor 3 2010-07-01 2010-07-01 false A country elevator may sell products and services to... Elevator § 780.710 A country elevator may sell products and services to farmers. Section 13(b)(14... the exemption, includes “such an establishment which sells products and services used in the...

  18. 12 CFR 150.370 - May I sell assets or lend money between fiduciary accounts?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 12 Banks and Banking 1 2012-01-01 2012-01-01 false May I sell assets or lend money between... Dealing § 150.370 May I sell assets or lend money between fiduciary accounts? You may sell assets or lend money between fiduciary accounts, if the transaction is fair to both accounts and is not prohibited...

  19. 12 CFR 550.370 - May I sell assets or lend money between fiduciary accounts?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 12 Banks and Banking 5 2011-01-01 2011-01-01 false May I sell assets or lend money between... Dealing § 550.370 May I sell assets or lend money between fiduciary accounts? You may sell assets or lend money between fiduciary accounts, if the transaction is fair to both accounts and is not prohibited...

  20. 12 CFR 550.370 - May I sell assets or lend money between fiduciary accounts?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 5 2010-01-01 2010-01-01 false May I sell assets or lend money between... Dealing § 550.370 May I sell assets or lend money between fiduciary accounts? You may sell assets or lend money between fiduciary accounts, if the transaction is fair to both accounts and is not prohibited...

  1. "!Chalinas a 20 Pesos!": Economic Ideas Developed through Children's Strategies for Successful Selling in Oaxaca, Mexico

    ERIC Educational Resources Information Center

    Sitabkhan, Yasmin Abdul

    2012-01-01

    The purpose of this dissertation is to explore the economic ideas of indigenous Triqui children between the ages of 5-15 who sell artisanal goods in Oaxaca, Mexico. I report findings from two studies that investigated (1) sellers' strategies for successfully selling goods, and (2) children's economic ideas linked to their selling strategies. In…

  2. 18 CFR 292.312 - Termination of obligation to sell to qualifying facilities.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... sell electric energy to a qualifying small power production facility, an existing qualifying...) Competing retail electric suppliers are willing and able to sell and deliver electric energy to the... is not required by State law to sell electric energy in its service territory....

  3. 18 CFR 292.312 - Termination of obligation to sell to qualifying facilities.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... sell electric energy to a qualifying small power production facility, an existing qualifying...) Competing retail electric suppliers are willing and able to sell and deliver electric energy to the... is not required by State law to sell electric energy in its service territory....

  4. 18 CFR 292.312 - Termination of obligation to sell to qualifying facilities.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... sell electric energy to a qualifying small power production facility, an existing qualifying...) Competing retail electric suppliers are willing and able to sell and deliver electric energy to the... is not required by State law to sell electric energy in its service territory....

  5. 18 CFR 292.312 - Termination of obligation to sell to qualifying facilities.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... sell electric energy to a qualifying small power production facility, an existing qualifying...) Competing retail electric suppliers are willing and able to sell and deliver electric energy to the... is not required by State law to sell electric energy in its service territory....

  6. A Framework for Personalized Dynamic Cross-Selling in E-Commerce Retailing

    ERIC Educational Resources Information Center

    Timalsina, Arun Kumar

    2012-01-01

    Cross-selling and product bundling are prevalent strategies in the retail sector. Instead of static bundling offers, i.e. giving the same offer to everyone, personalized dynamic cross-selling generates targeted bundle offers and can help maximize revenues and profits. In resolving the two basic problems of dynamic cross-selling, which involves…

  7. 17 CFR 242.102 - Activities by issuers and selling security holders during a distribution.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... selling security holders during a distribution. 242.102 Section 242.102 Commodity and Securities Exchanges... REQUIREMENTS FOR SECURITY FUTURES Regulation M § 242.102 Activities by issuers and selling security holders... or on behalf of an issuer or selling security holder, it shall be unlawful for such person, or...

  8. Modified TAROT for cross-selling personal financial products

    NASA Astrophysics Data System (ADS)

    Tee, Ya-Mei; LEE, Lai-Soon; LEE, Chew-Ging; SEOW, Hsin-Vonn

    2014-09-01

    The Top Application characteristics Remainder Offer characteristics Tree (TAROT) was first introduced in 2007. This is a modified Classification and Regression Trees (CART) used to help decide which question(s) to ask potential applicants to customise an offer of a personal financial product so that it would have a high probability of take up. In this piece of work the authors are presenting, they have further modified the TAROT to cross TAROT, using its properties and modeling steps to deal with the issue of cross-selling. Since the bank already has ready customers, it would be ideal to cross-sell the financial products seeing that one can ask one (or more) further question(s) based on the initial offer to identify and customise another financial product to offer.

  9. Why do rural primary care physicians sell their practices?

    PubMed

    Stensland, Jeffrey; Brasure, Michelle; Moscovice, Ira

    2002-01-01

    This study evaluates why rural primary care physicians sell their practices. A random sample of rural primary care practices in California, Utah, Ohio, Texas, and Virginia were surveyed to investigate changes in ownership of the practices during the period 1995-1998. These five states were selected because they represent areas with different experiences with physician-hospital integration and varied rates of managed care penetration. A series of logistic regressions were conducted to examine the factors that led independent physicians to sell their practices to either nonlocal buyers, local hospitals, or local physicians. Findings suggest that sales to nonlocal buyers represent the majority of practice ownership changes. The motivations for ceding control to nonlocal buyers center on managed care concerns, recruitment concerns, and administrative burdens. Sellers were also concerned about their level of net income prior to being acquired. However, the preacquisition financial concerns of sellers were not significantly stronger than the financial concerns of practices that remained independent. The environmental conditions that motivate rural physicians to sell their practices are not expected to improve. Therefore, additional sales of rural primary care practices to nonlocal buyers are expected. Further research is necessary to determine whether this shift in control will lead to changes in the quality or accessibility of care.

  10. 30 CFR 1206.102 - How do I calculate royalty value for oil that I or my affiliate sell(s) under an arm's-length...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 30 Mineral Resources 3 2011-07-01 2011-07-01 false How do I calculate royalty value for oil that I... Revenue PRODUCT VALUATION Federal Oil § 1206.102 How do I calculate royalty value for oil that I or my affiliate sell(s) under an arm's-length contract? (a) The value of oil under this section is the...

  11. 30 CFR 1206.52 - How do I calculate royalty value for oil that I or my affiliate sell(s) or exchange(s) under an...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 30 Mineral Resources 3 2013-07-01 2013-07-01 false How do I calculate royalty value for oil that I or my affiliate sell(s) or exchange(s) under an arm's-length contract? 1206.52 Section 1206.52 Mineral Resources OFFICE OF NATURAL RESOURCES REVENUE, DEPARTMENT OF THE INTERIOR NATURAL...

  12. 30 CFR 1206.102 - How do I calculate royalty value for oil that I or my affiliate sell(s) under an arm's-length...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 30 Mineral Resources 3 2014-07-01 2014-07-01 false How do I calculate royalty value for oil that I or my affiliate sell(s) under an arm's-length contract? 1206.102 Section 1206.102 Mineral Resources OFFICE OF NATURAL RESOURCES REVENUE, DEPARTMENT OF THE INTERIOR NATURAL RESOURCES REVENUE...

  13. 30 CFR 1206.52 - How do I calculate royalty value for oil that I or my affiliate sell(s) or exchange(s) under an...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 30 Mineral Resources 3 2012-07-01 2012-07-01 false How do I calculate royalty value for oil that I or my affiliate sell(s) or exchange(s) under an arm's-length contract? 1206.52 Section 1206.52 Mineral Resources OFFICE OF NATURAL RESOURCES REVENUE, DEPARTMENT OF THE INTERIOR NATURAL...

  14. 30 CFR 1206.52 - How do I calculate royalty value for oil that I or my affiliate sell(s) or exchange(s) under an...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 30 Mineral Resources 3 2014-07-01 2014-07-01 false How do I calculate royalty value for oil that I or my affiliate sell(s) or exchange(s) under an arm's-length contract? 1206.52 Section 1206.52 Mineral Resources OFFICE OF NATURAL RESOURCES REVENUE, DEPARTMENT OF THE INTERIOR NATURAL...

  15. 30 CFR 1206.102 - How do I calculate royalty value for oil that I or my affiliate sell(s) under an arm's-length...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 30 Mineral Resources 3 2013-07-01 2013-07-01 false How do I calculate royalty value for oil that I or my affiliate sell(s) under an arm's-length contract? 1206.102 Section 1206.102 Mineral Resources OFFICE OF NATURAL RESOURCES REVENUE, DEPARTMENT OF THE INTERIOR NATURAL RESOURCES REVENUE...

  16. 30 CFR 1206.102 - How do I calculate royalty value for oil that I or my affiliate sell(s) under an arm's-length...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 30 Mineral Resources 3 2012-07-01 2012-07-01 false How do I calculate royalty value for oil that I or my affiliate sell(s) under an arm's-length contract? 1206.102 Section 1206.102 Mineral Resources OFFICE OF NATURAL RESOURCES REVENUE, DEPARTMENT OF THE INTERIOR NATURAL RESOURCES REVENUE...

  17. Social context of needle selling in Baltimore, Maryland.

    PubMed

    Latkin, Carl A; Davey, Melissa A; Hua, Wei

    2006-01-01

    Although much of the debate surrounding the distribution of sterile syringes to injection drug users (IDUs) has focused on needle exchange programs (NEPs), IDUs acquire their syringes from three major sources: NEPs, pharmacies, and secondary exchangers or needle sellers. The purpose of the present study is to examine types and frequencies of social interactions among drug injectors who sell needles, most of which come from NEPs, compared with individuals who do not sell needles. Specifically, we compared engagement in drug-related behaviors, roles in the drug economy, and social network membership. Data were collected as part of the SHIELD study, an HIV prevention intervention targeted at drug users and their social networks (n=910) from February 2001 through September 2003 in Baltimore, Maryland (USA). In this sample, 56 participants reported selling needles. Needle sellers had higher levels of engagement in drug-related social interactions, including using drugs with others, giving or receiving drugs from others, and buying drugs with other users. Participants who sold needles had a significantly higher number of roles in the drug economy. Also, they had more social network members who were injectors, with whom they talked about risky drug behaviors, gave needles to, and shared cookers and bleach with. Compared with nonselling injectors, needle sellers engage in HIV risk-related behaviors, such as injecting daily and sharing injection equipment, more frequently. The study's findings may be useful to determine whether secondary exchangers should be targeted for HIV prevention activities both to reduce their own risk and to diffuse risk reduction information throughout the drug using community.

  18. [Legal aspects of selling medical products by gynecologists].

    PubMed

    Urbaniak, Monika; Spaczyński, Robert Z

    2013-07-01

    Sales and distribution of medical products and drugs in Poland remains under strict regulations, especially legal regulation contained in the Medical and Dental Practitioners Act, that banned sales of medical products by doctors. It needs to be emphasized that currently doctors are allowed to sell drugs and medical products only in rigorously specified situations. Knowledge of current legal regulations concerning sales of medical products by gynecologists allows to conform with the law and to distribute drugs and medical products under special and predefined conditions.

  19. Time dependent optimal switching controls in online selling models

    SciTech Connect

    Bradonjic, Milan; Cohen, Albert

    2010-01-01

    We present a method to incorporate dishonesty in online selling via a stochastic optimal control problem. In our framework, the seller wishes to maximize her average wealth level W at a fixed time T of her choosing. The corresponding Hamilton-Jacobi-Bellmann (HJB) equation is analyzed for a basic case. For more general models, the admissible control set is restricted to a jump process that switches between extreme values. We propose a new approach, where the optimal control problem is reduced to a multivariable optimization problem.

  20. Cross-correlations in volume space: Differences between buy and sell volumes

    NASA Astrophysics Data System (ADS)

    Lee, Sun Young; Hwang, Dong Il; Kim, Min Jae; Koh, In Gyu; Kim, Soo Yong

    2011-03-01

    We study the cross-correlations of buy and sell volumes on the Korean stock market in high frequency. We observe that the pulling effects of volumes are as small as that of returns. The properties of the correlations of buy and sell volumes differ. They are explained by the degree of synchronization of stock volumes. Further, the pulling effects on the minimal spanning tree are studied. In minimal spanning trees with directed links, the large pulling effects are clustered at the center, not uniformly distributed. The Epps effect of buy and sell volumes are observed. The reversal of the cross-correlations of buy and sell volumes is also detected.

  1. The speakers’ bureau system: a form of peer selling

    PubMed Central

    Reid, Lynette; Herder, Matthew

    2013-01-01

    Abstract In the speakers’ bureau system, physicians are recruited and trained by pharmaceutical, biotechnology, and medical device companies to deliver information about products to other physicians, in exchange for a fee. Using publicly available disclosures, we assessed the thesis that speakers’ bureau involvement is not a feature of academic medicine in Canada, by estimating the prevalence of participation in speakers’ bureaus among Canadian faculty in one medical specialty, cardiology. We analyzed the relevant features of an actual contract made public by the physician addressee and applied the Canadian Medical Association (CMA) guidelines on physician–industry relations to participation in a speakers’ bureau. We argue that speakers’ bureau participation constitutes a form of peer selling that should be understood to contravene the prohibition on product endorsement in the CMA Code of Ethics. Academic medical institutions, in conjunction with regulatory colleges, should continue and strengthen their policies to address participation in speakers’ bureaus. PMID:24348883

  2. Selling health data: de-identification, privacy, and speech.

    PubMed

    Kaplan, Bonnie

    2015-07-01

    Two court cases that involve selling prescription data for pharmaceutical marketing affect biomedical informatics, patient and clinician privacy, and regulation. Sorrell v. IMS Health Inc. et al. in the United States and R v. Department of Health, Ex Parte Source Informatics Ltd. in the United Kingdom concern privacy and health data protection, data de-identification and reidentification, drug detailing (marketing), commercial benefit from the required disclosure of personal information, clinician privacy and the duty of confidentiality, beneficial and unsavory uses of health data, regulating health technologies, and considering data as speech. Individuals should, at the very least, be aware of how data about them are collected and used. Taking account of how those data are used is needed so societal norms and law evolve ethically as new technologies affect health data privacy and protection.

  3. Eleven essential steps to purchasing or selling a medical practice.

    PubMed

    Barrett, William

    2014-01-01

    Based on our experience in representing more than 100 doctors and medical specialists in practice sales and acquisitions, we have identified 11 key considerations important to a deal. There are several issues to consider while going through the process of buying or selling a practice including the implementation of a "letter of intent" as a first step rather than drafting a contract, securing a lease, and verifying the property is not in violation of the local zoning requirements. There are also considerations with regard to the patients, which range from how will the accounts receivable at the time of the closing be handled to who is responsible for the handling of continued treatment in an ongoing case after a deal is finalized. This article details these considerations and more.

  4. 30 CFR 1206.52 - How do I calculate royalty value for oil that I or my affiliate sell(s) or exchange(s) under an...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 30 Mineral Resources 3 2011-07-01 2011-07-01 false How do I calculate royalty value for oil that I... Resources Revenue PRODUCT VALUATION Indian Oil § 1206.52 How do I calculate royalty value for oil that I or my affiliate sell(s) or exchange(s) under an arm's-length contract? (a) The value of oil under...

  5. Close It, Sell It, or Lease It: What to Do with That Old School.

    ERIC Educational Resources Information Center

    Smith, Walter

    Recommendations on leasing or selling a surplus school building that has been closed due to declining enrollment are offered. Questions which need to be addressed in deciding which option to pursue are presented including whether it would be better to use the building for community purposes than to lease or sell it. The advantages and…

  6. 43 CFR 3602.41 - When will BLM sell mineral materials on a competitive basis?

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 43 Public Lands: Interior 2 2012-10-01 2012-10-01 false When will BLM sell mineral materials on a... (Continued) BUREAU OF LAND MANAGEMENT, DEPARTMENT OF THE INTERIOR MINERALS MANAGEMENT (3000) MINERAL MATERIALS DISPOSAL Mineral Materials Sales Competitive Sales § 3602.41 When will BLM sell mineral...

  7. 26 CFR 1.1234-1 - Options to buy or sell.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... option determines whether the capital gain or loss is short-term or long-term. (2) Section 1231... Options to buy or sell. (a) Sale or exchange—(1) Capital assets. Gain or loss from the sale or exchange of an option (or privilege) to buy or sell property which is (or if acquired would be) a capital...

  8. 27 CFR 31.66 - Retail dealer selling entire stock in liquidation.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... entire stock in liquidation. 31.66 Section 31.66 Alcohol, Tobacco Products and Firearms ALCOHOL AND... Exceptions Persons Who Are Not Dealers in Liquors Or Beer § 31.66 Retail dealer selling entire stock in... liquors or a wholesale dealer in beer by virtue of selling in liquidation that dealer's entire stock...

  9. 27 CFR 31.64 - Apothecaries or druggists selling medicines and tinctures.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... selling medicines and tinctures. 31.64 Section 31.64 Alcohol, Tobacco Products and Firearms ALCOHOL AND... Exceptions Persons Who Are Not Dealers in Liquors Or Beer § 31.64 Apothecaries or druggists selling medicines... medicines and in making tinctures that are unfit for use for beverage purposes are not considered to...

  10. 27 CFR 31.64 - Apothecaries or druggists selling medicines and tinctures.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... selling medicines and tinctures. 31.64 Section 31.64 Alcohol, Tobacco Products and Firearms ALCOHOL AND... Exceptions Persons Who Are Not Dealers in Liquors Or Beer § 31.64 Apothecaries or druggists selling medicines... medicines and in making tinctures that are unfit for use for beverage purposes are not considered to...

  11. 27 CFR 31.64 - Apothecaries or druggists selling medicines and tinctures.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... selling medicines and tinctures. 31.64 Section 31.64 Alcohol, Tobacco Products and Firearms ALCOHOL AND... Exceptions Persons Who Are Not Dealers in Liquors Or Beer § 31.64 Apothecaries or druggists selling medicines... medicines and in making tinctures that are unfit for use for beverage purposes are not considered to...

  12. A New Approach to Organizing and Selling Advertising for the School Paper

    ERIC Educational Resources Information Center

    Broad, Shirley C.

    1977-01-01

    Describes ways in which a high school newspaper reorganized its approach to designing advertising layouts and selling advertising space. The new approach includes the use of "unitized" page samples to show to advertisers and the preparation of advertising packets for use by staff members in selling ads. (GW)

  13. 43 CFR 3602.41 - When will BLM sell mineral materials on a competitive basis?

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 43 Public Lands: Interior 2 2013-10-01 2013-10-01 false When will BLM sell mineral materials on a... (Continued) BUREAU OF LAND MANAGEMENT, DEPARTMENT OF THE INTERIOR MINERALS MANAGEMENT (3000) MINERAL MATERIALS DISPOSAL Mineral Materials Sales Competitive Sales § 3602.41 When will BLM sell mineral...

  14. 43 CFR 3602.41 - When will BLM sell mineral materials on a competitive basis?

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 43 Public Lands: Interior 2 2014-10-01 2014-10-01 false When will BLM sell mineral materials on a... (Continued) BUREAU OF LAND MANAGEMENT, DEPARTMENT OF THE INTERIOR MINERALS MANAGEMENT (3000) MINERAL MATERIALS DISPOSAL Mineral Materials Sales Competitive Sales § 3602.41 When will BLM sell mineral...

  15. 43 CFR 3602.41 - When will BLM sell mineral materials on a competitive basis?

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 43 Public Lands: Interior 2 2011-10-01 2011-10-01 false When will BLM sell mineral materials on a... (Continued) BUREAU OF LAND MANAGEMENT, DEPARTMENT OF THE INTERIOR MINERALS MANAGEMENT (3000) MINERAL MATERIALS DISPOSAL Mineral Materials Sales Competitive Sales § 3602.41 When will BLM sell mineral...

  16. 9 CFR 201.61 - Market agencies selling or purchasing livestock on commission; relationships with dealers.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... livestock on commission; relationships with dealers. 201.61 Section 201.61 Animals and Animal Products GRAIN... or purchasing livestock on commission; relationships with dealers. (a) Market agencies selling on commission. No market agency selling consigned livestock shall enter into any agreement, relationship...

  17. 25 CFR 161.711 - How will BIA sell impounded livestock or other property?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 25 Indians 1 2010-04-01 2010-04-01 false How will BIA sell impounded livestock or other property... NAVAJO PARTITIONED LANDS GRAZING PERMITS Trespass Actions § 161.711 How will BIA sell impounded livestock or other property? (a) Unless the owner or known lien holder of the impounded livestock or...

  18. 27 CFR 31.64 - Apothecaries or druggists selling medicines and tinctures.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... selling medicines and tinctures. 31.64 Section 31.64 Alcohol, Tobacco Products and Firearms ALCOHOL AND... Exceptions Persons Who Are Not Dealers in Liquors Or Beer § 31.64 Apothecaries or druggists selling medicines... medicines and in making tinctures that are unfit for use for beverage purposes are not considered to...

  19. 27 CFR 31.64 - Apothecaries or druggists selling medicines and tinctures.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... selling medicines and tinctures. 31.64 Section 31.64 Alcohol, Tobacco Products and Firearms ALCOHOL AND... Exceptions Persons Who Are Not Dealers in Liquors Or Beer § 31.64 Apothecaries or druggists selling medicines... medicines and in making tinctures that are unfit for use for beverage purposes are not considered to...

  20. 17 CFR 250.87 - Subsidiaries authorized to perform services or construction or to sell goods.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... investment trust; or (2) Such services, construction, or goods are reasonably required by such associate to..., or sell goods to, associate companies thereof: (1) An approved mutual service company. (2) A... businesses other than that of selling goods to associate companies, that of performing services...

  1. 27 CFR 31.66 - Retail dealer selling entire stock in liquidation.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Retail dealer selling... Exceptions Persons Who Are Not Dealers in Liquors Or Beer § 31.66 Retail dealer selling entire stock in liquidation. No retail dealer in liquors or retail dealer in beer shall be deemed to be a wholesale dealer...

  2. 17 CFR 242.105 - Short selling in connection with a public offering.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 17 Commodity and Securities Exchanges 3 2010-04-01 2010-04-01 false Short selling in connection with a public offering. 242.105 Section 242.105 Commodity and Securities Exchanges SECURITIES AND... SECURITY FUTURES Regulation M § 242.105 Short selling in connection with a public offering. (a)...

  3. 9 CFR 201.67 - Packers not to own or finance selling agencies.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 9 Animals and Animal Products 2 2014-01-01 2014-01-01 false Packers not to own or finance selling... STOCKYARDS ACT Trade Practices § 201.67 Packers not to own or finance selling agencies. No packer subject to the Act shall have an ownership interest in, finance, or participate in the management or operation...

  4. 9 CFR 201.67 - Packers not to own or finance selling agencies.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 9 Animals and Animal Products 2 2011-01-01 2011-01-01 false Packers not to own or finance selling... STOCKYARDS ACT Trade Practices § 201.67 Packers not to own or finance selling agencies. No packer subject to the Act shall have an ownership interest in, finance, or participate in the management or operation...

  5. 9 CFR 201.67 - Packers not to own or finance selling agencies.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 9 Animals and Animal Products 2 2013-01-01 2013-01-01 false Packers not to own or finance selling... STOCKYARDS ACT Trade Practices § 201.67 Packers not to own or finance selling agencies. No packer subject to the Act shall have an ownership interest in, finance, or participate in the management or operation...

  6. 9 CFR 201.67 - Packers not to own or finance selling agencies.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 9 Animals and Animal Products 2 2012-01-01 2012-01-01 false Packers not to own or finance selling... STOCKYARDS ACT Trade Practices § 201.67 Packers not to own or finance selling agencies. No packer subject to the Act shall have an ownership interest in, finance, or participate in the management or operation...

  7. 9 CFR 201.67 - Packers not to own or finance selling agencies.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Packers not to own or finance selling... STOCKYARDS ACT Trade Practices § 201.67 Packers not to own or finance selling agencies. No packer subject to the Act shall have an ownership interest in, finance, or participate in the management or operation...

  8. Factors Associated With American Indian and White Adolescent Drug Selling in Rural Communities

    PubMed Central

    Eitle, David; Eitle, Tamela McNulty

    2014-01-01

    Relatively few studies have examined the correlates of adolescent drug selling in America, with most of these studies focusing on urban settings. The present study examines the risk and protective factors associated with drug selling among American Indian and white adolescents residing in a rural Northwestern state in the United States. Using survey data collected in 2010-2012, we conduct logistic regression analyses exploring the correlates of drug selling (n=568). Generally, we found support for prior explanations of drug selling, but identified some important race-specific differences. Specifically, we found that stress exposure was a risk factor for American Indians, but not whites. Conversely, academic achievement served as a protective factor for white adolescents but not American Indians. Our findings suggest that the race gap in rural drug selling can be explained by considering differences in social bonds, stress exposure, and exposure to substance using family and friends. PMID:26120365

  9. 12 CFR 617.7620 - What should the System institution do when it decides to sell acquired agricultural real estate...

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... decides to sell acquired agricultural real estate at a public auction? 617.7620 Section 617.7620 Banks and... What should the System institution do when it decides to sell acquired agricultural real estate at a public auction? System institutions electing to sell or lease acquired agricultural real estate or...

  10. 12 CFR 617.7610 - What should the System institution do when it decides to sell acquired agricultural real estate?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... decides to sell acquired agricultural real estate? 617.7610 Section 617.7610 Banks and Banking FARM CREDIT... institution do when it decides to sell acquired agricultural real estate? (a) Notify the previous owner, (1) Within 15 days of the System institution's decision to sell acquired agricultural real estate, it...

  11. 12 CFR 617.7610 - What should the System institution do when it decides to sell acquired agricultural real estate?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... decides to sell acquired agricultural real estate? 617.7610 Section 617.7610 Banks and Banking FARM CREDIT... institution do when it decides to sell acquired agricultural real estate? (a) Notify the previous owner, (1) Within 15 days of the System institution's decision to sell acquired agricultural real estate, it...

  12. 12 CFR 617.7610 - What should the System institution do when it decides to sell acquired agricultural real estate?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... decides to sell acquired agricultural real estate? 617.7610 Section 617.7610 Banks and Banking FARM CREDIT... institution do when it decides to sell acquired agricultural real estate? (a) Notify the previous owner, (1) Within 15 days of the System institution's decision to sell acquired agricultural real estate, it...

  13. 12 CFR 617.7610 - What should the System institution do when it decides to sell acquired agricultural real estate?

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... decides to sell acquired agricultural real estate? 617.7610 Section 617.7610 Banks and Banking FARM CREDIT... institution do when it decides to sell acquired agricultural real estate? (a) Notify the previous owner, (1) Within 15 days of the System institution's decision to sell acquired agricultural real estate, it...

  14. 17 CFR 1.39 - Simultaneous buying and selling orders of different principals; execution of, for and between...

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... selling orders of different principals; execution of, for and between principals. 1.39 Section 1.39... COMMODITY EXCHANGE ACT Recordkeeping § 1.39 Simultaneous buying and selling orders of different principals... shall have in hand at the same time both buying and selling orders of different principals for the...

  15. 17 CFR 1.39 - Simultaneous buying and selling orders of different principals; execution of, for and between...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... selling orders of different principals; execution of, for and between principals. 1.39 Section 1.39... COMMODITY EXCHANGE ACT Recordkeeping § 1.39 Simultaneous buying and selling orders of different principals... shall have in hand at the same time both buying and selling orders of different principals for the...

  16. 17 CFR 1.39 - Simultaneous buying and selling orders of different principals; execution of, for and between...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... selling orders of different principals; execution of, for and between principals. 1.39 Section 1.39... COMMODITY EXCHANGE ACT Recordkeeping § 1.39 Simultaneous buying and selling orders of different principals... shall have in hand at the same time both buying and selling orders of different principals for the...

  17. Preference for art: similarity, statistics, and selling price

    NASA Astrophysics Data System (ADS)

    Graham, Daniel J.; Friedenberg, Jay D.; McCandless, Cyrus H.; Rockmore, Daniel N.

    2010-02-01

    preference, one might expect "free markets" to value art in proportion to its aesthetic appeal, at least to some extent. To assess the role of value, a further test of preference was performed on a separate set of paintings recently sold at auction. Results showed that the selling price of these works showed no correlation with preference, while basic statistics were significantly correlated with preference. We conclude that selling price, which could be seen as a proxy for a painting's "value," is not predictive of preference, while shared preferences may to some extent be predictable based on image statistics. We also suggest that contextual and semantic factors play an important role in preference given that image content appears to lead to greater divergence between similarity and preference ratings for representational works, and especially for artwork that prominently depicts faces. The present paper paves the way for a more complete understanding of the relationship between shared human preferences and image statistical regularities, and it outlines the basic geometry of perceptual spaces for artwork.

  18. It`s time to sell the TVA

    SciTech Connect

    Malec, W.F.

    1996-03-01

    The electric power functions of the Tennessee Valley Authority should be sold now, in total, says a former top TVA executive - not because the agency can`t survive as is, but because doing so would produce a better result for TVA`s customers and employees, as well as U.S. taxpayers. It seems ironic to the author that while the U.S. generally encourages the spread of free enterprise, and as we are witnessing an unprecedented privatization of many formerly government-owned utility systems around the world, the U.S. government retains ownership of one of the largest electric companies in the U.S. and the world in the Tennessee Valley Authority. In many areas of the globe, privatization is moving with strong investment from U.S. firms and quasi-governmental institutions like the World Bank, yet our government won`t seriously consider selling the TVA. The author believes such a sale would be beneficial in many ways. It would generate big savings for the U.S. taxpayer. It would reduce the federal debt, and build on massive operating and financial progress made at TVA in the last seven years. It might also provide a model of how and why to privatize other parts of the federal government. At the same time it would move one of the largest electric companies in America out from under the burden of federal bureaucracy into the private sector, where the author believes it could compete effectively, without excuses or alibis.

  19. Selling the Drama: Army Marketing Strategies and the Future of Word-of-Mouth Marketing

    DTIC Science & Technology

    2017-02-16

    the Army’s marketing campaign from the perspective of a recruiter and a Noncommissioned Officer. SSG Darling hopes to inspire the readers of this...essay to get out, tell the Army’s story, and to contribute to the recruitment of quality Soldiers. “Selling the Drama”: Army Marketing ...Strategies and the Future of Word-of-Mouth Marketing Word Count: 2,533 “Selling the Drama”: Army Marketing Strategies and the Future of Word-of-Mouth

  20. 26 CFR 1.1234-1 - Options to buy or sell.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 26 Internal Revenue 11 2010-04-01 2010-04-01 true Options to buy or sell. 1.1234-1 Section 1.1234... (CONTINUED) INCOME TAXES Special Rules for Determining Capital Gains and Losses § 1.1234-1 Options to buy or... privilege) to buy or sell property which is (or if acquired would be) a capital asset in the hands of...

  1. The search for organs: halachic perspectives on altruistic giving and the selling of organs

    PubMed Central

    Kunin, J

    2005-01-01

    Altruistic donation of organs from living donors is widely accepted as a virtue and even encouraged as a duty. Selling organs, on the other hand, is highly controversial and banned in most countries. What is the Jewish legal (halachic) position on these issues? In this review it is explained that altruistic donation is praiseworthy but in no way obligatory. Selling organs is a subject of rabbinic dispute among contemporary authorities. PMID:15863684

  2. Factors affecting the selling price of feeder cattle sold at Arkansas livestock auctions in 2005.

    PubMed

    Barham, B L; Troxel, T R

    2007-12-01

    Data were collected from 15 Arkansas livestock auctions to determine factors affecting selling price. Data included how calves were sold (single or groups), sex, breed or breed type, color, muscle thickness, horn status, frame score, fill, body condition, age, health, BW, and price. Data were randomly collected on 52,401 lots consisting of 105,542 calves. Selling prices for steers ($124.20 +/- 0.07), bulls ($117.93 +/- 0.12), and heifers ($112.81 +/- 0.07) were different from each other (P <0.001). Hereford x Charolais feeder calves sold for the highest price ($122.66 +/- 0.14) and Longhorns sold for the lowest price ($74.52 +/- 0.46). Yellow feeder cattle received the highest selling price ($96.47 +/- 0.12), and spotted or striped feeder cattle received the lowest price ($83.84 +/- 0.23). The selling price of singles was lower than the price for calves sold in groups of 6 or more ($117.26 +/- 0.06 vs. $122.61 +/- 0.21; P <0.001). For cattle classified as having muscle scores of 1, 2, 3, and 4, selling prices were $120.45 +/- 0.05, $111.31 +/- 0.09, $96.28 +/- 0.44, and $82.21 +/- 1.87, respectively. Polled feeder cattle sold for $118.57 +/- 0.05, and horned feeder cattle sold for $114.87 +/- 0.14 (P <0.001). Interactions (P <0.001) were detected between frame score and BW groups, and muscle score and BW groups on the selling price of cattle. A number of management and genetic factors affected the selling price of feeder cattle.

  3. 17 CFR 230.135b - Materials not deemed an offer to sell or offer to buy nor a prospectus.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... to sell or offer to buy nor a prospectus. 230.135b Section 230.135b Commodity and Securities... § 230.135b Materials not deemed an offer to sell or offer to buy nor a prospectus. Materials meeting the requirements of § 240.9b-1 of this chapter shall not be deemed an offer to sell or offer to buy a security...

  4. Serious Delinquency and Gang Participation: Combining and Specializing in Drug Selling, Theft and Violence

    PubMed Central

    Gordon, Rachel A.; Rowe, Hillary L.; Pardini, Dustin; Loeber, Rolf; White, Helene Raskin; Farrington, David P.

    2014-01-01

    Using Pittsburgh Youth Study data, we examined the extent to which over 600 gang members and non-gang involved young men specialized in drug selling, serious theft, or serious violence or engaged simultaneously in these serious delinquent behaviors, throughout the 1990s. We found that the increase in delinquency associated with gang membership was concentrated in two combinations: serious violence and drug selling; serious violence, drug selling, and serious theft. Several covariates were similarly associated with multi-type serious delinquency and gang membership (age, historical time, Black race, and residential mobility), suggesting that these behaviors may share common developmental, familial, and contextual risks. We encourage future research to further examine the association of gang membership with engagement in particular configurations of serious delinquency. PMID:24954999

  5. Serious Delinquency and Gang Participation: Combining and Specializing in Drug Selling, Theft and Violence.

    PubMed

    Gordon, Rachel A; Rowe, Hillary L; Pardini, Dustin; Loeber, Rolf; White, Helene Raskin; Farrington, David P

    2014-06-01

    Using Pittsburgh Youth Study data, we examined the extent to which over 600 gang members and non-gang involved young men specialized in drug selling, serious theft, or serious violence or engaged simultaneously in these serious delinquent behaviors, throughout the 1990s. We found that the increase in delinquency associated with gang membership was concentrated in two combinations: serious violence and drug selling; serious violence, drug selling, and serious theft. Several covariates were similarly associated with multi-type serious delinquency and gang membership (age, historical time, Black race, and residential mobility), suggesting that these behaviors may share common developmental, familial, and contextual risks. We encourage future research to further examine the association of gang membership with engagement in particular configurations of serious delinquency.

  6. The Prevalence of Phosphorus Containing Food Additives in Top Selling Foods in Grocery Stores

    PubMed Central

    León, Janeen B.; Sullivan, Catherine M.; Sehgal, Ashwini R.

    2013-01-01

    Objective To determine the prevalence of phosphorus-containing food additives in best selling processed grocery products and to compare the phosphorus content of a subset of top selling foods with and without phosphorus additives. Design The labels of 2394 best selling branded grocery products in northeast Ohio were reviewed for phosphorus additives. The top 5 best selling products containing phosphorus additives from each food category were matched with similar products without phosphorus additives and analyzed for phosphorus content. Four days of sample meals consisting of foods with and without phosphorus additives were created and daily phosphorus and pricing differentials were computed. Setting Northeast Ohio Main outcome measures Presence of phosphorus-containing food additives, phosphorus content Results 44% of the best selling grocery items contained phosphorus additives. The additives were particularly common in prepared frozen foods (72%), dry food mixes (70%), packaged meat (65%), bread & baked goods (57%), soup (54%), and yogurt (51%) categories. Phosphorus additive containing foods averaged 67 mg phosphorus/100 gm more than matched non-additive containing foods (p=.03). Sample meals comprised mostly of phosphorus additive-containing foods had 736 mg more phosphorus per day compared to meals consisting of only additive-free foods. Phosphorus additive-free meals cost an average of $2.00 more per day. Conclusion Phosphorus additives are common in best selling processed groceries and contribute significantly to their phosphorus content. Moreover, phosphorus additive foods are less costly than phosphorus additive-free foods. As a result, persons with chronic kidney disease may purchase these popular low-cost groceries and unknowingly increase their intake of highly bioavailable phosphorus. PMID:23402914

  7. Buying and Selling Stocks of Multi Brands Using Genetic Network Programming with Control Nodes

    NASA Astrophysics Data System (ADS)

    Ohkawa, Etsushi; Chen, Yan; Bao, Zhiguo; Mabu, Shingo; Shimada, Kaoru; Hirasawa, Kotaro

    A new evolutionary method named “Genetic Network Programming with control nodes, GNPcn” has been applied to determine the timing of buying or selling stocks. GNPcn represents its solutions as directed graph structures which has some useful features inherently. For example, GNPcn has an implicit memory function which memorizes the past action sequences of agents and GNPcn can re-use nodes repeatedly in the network flow, so very compact graph structures can be made. GNPcn can determine the strategy of buying and selling stocks of multi issues. The effectiveness of the proposed method is confirmed by simulations.

  8. 12 CFR 617.7610 - What should the System institution do when it decides to sell acquired agricultural real estate?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 12 Banks and Banking 7 2012-01-01 2012-01-01 false What should the System institution do when it... institution do when it decides to sell acquired agricultural real estate? (a) Notify the previous owner, (1) Within 15 days of the System institution's decision to sell acquired agricultural real estate, it...

  9. Selling and Implementing Leadership Development: Chapter, Verse, and Lessons Learned from Carlson's Story

    ERIC Educational Resources Information Center

    Clevette, Rick D.; Cohen, Stephen L.

    2007-01-01

    This article provides a case study template for creating and "selling" an enterprise-wide leadership development initiative in a highly decentralized organization. Using a story-telling approach, the authors delineate the five separate but highly interdependent stages used to achieve this. Sandwiched between a prologue and epilogue are five…

  10. Using Sales Management Students to Manage Professional Selling Students in an Innovative Active Learning Project

    ERIC Educational Resources Information Center

    Young, Joyce A.; Hawes, Jon M.

    2013-01-01

    This paper describes an application of active learning within two different courses: professional selling and sales management. Students assumed the roles of sales representatives and sales managers for an actual fund-raiser--a golf outing--sponsored by a student chapter of the American Marketing Association. The sales project encompassed an…

  11. Publishers See Online Mega-Courses as Opportunity to Sell Textbooks

    ERIC Educational Resources Information Center

    Howard, Jennifer

    2012-01-01

    Colleges are not the only enterprises interested in the possibilities of free, online courses. Publishers have begun to investigate whether so-called MOOC's, or massive open online courses, can help them reach new readers and sell more books. For the moment, providers of the classes encourage professors not to require students to buy texts, in…

  12. 9 CFR 201.56 - Market agencies selling on commission; purchases from consignment.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 9 Animals and Animal Products 2 2011-01-01 2011-01-01 false Market agencies selling on commission; purchases from consignment. 201.56 Section 201.56 Animals and Animal Products GRAIN INSPECTION, PACKERS AND... other available buyers, and then only at a price higher than the highest available bid on such...

  13. 9 CFR 201.56 - Market agencies selling on commission; purchases from consignment.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 9 Animals and Animal Products 2 2014-01-01 2014-01-01 false Market agencies selling on commission; purchases from consignment. 201.56 Section 201.56 Animals and Animal Products GRAIN INSPECTION, PACKERS AND... other available buyers, and then only at a price higher than the highest available bid on such...

  14. 9 CFR 201.56 - Market agencies selling on commission; purchases from consignment.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 9 Animals and Animal Products 2 2012-01-01 2012-01-01 false Market agencies selling on commission; purchases from consignment. 201.56 Section 201.56 Animals and Animal Products GRAIN INSPECTION, PACKERS AND... other available buyers, and then only at a price higher than the highest available bid on such...

  15. The Local Food Grower's Behavior during Planning, Growing, Harvesting, and Selling

    ERIC Educational Resources Information Center

    Gumirakiza, J. Dominique

    2016-01-01

    This article presents a perspective on the behavior of the local food grower during the market-related decision-making process. The relevant behavior is manifested during the planning, growing, and harvesting and selling phases of the agricultural business cycle. The local food grower faces a set of market alternatives from which, if applying a…

  16. The Personal Selling Ethics Scale: Revisions and Expansions for Teaching Sales Ethics

    ERIC Educational Resources Information Center

    Donoho, Casey; Heinze, Timothy

    2011-01-01

    The field of sales draws a large number of marketing graduates. Sales curricula used within today's marketing programs should include rigorous discussions of sales ethics. The Personal Selling Ethics Scale (PSE) provides an analytical tool for assessing and discussing students' ethical sales sensitivities. However, since the scale fails to address…

  17. Application of Contemporary Literature to Enhance Interpersonal Skills and Ethical Decision Making in Professional Selling Coursework

    ERIC Educational Resources Information Center

    Kimball, Bob

    2007-01-01

    Educators and marketing professionals agree that course-work must address interpersonal communication skills and ethical decision making in addition to traditional business functions and skills. This article describes an innovative approach to teaching the professional selling course in which students enhance their competency in these areas…

  18. 17 CFR 242.105 - Short selling in connection with a public offering.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 17 Commodity and Securities Exchanges 3 2011-04-01 2011-04-01 false Short selling in connection with a public offering. 242.105 Section 242.105 Commodity and Securities Exchanges SECURITIES AND EXCHANGE COMMISSION (CONTINUED) REGULATIONS M, SHO, ATS, AC, AND NMS AND CUSTOMER MARGIN REQUIREMENTS...

  19. Integrating the Concept of Customer Needs into an Undergraduate Professional Selling Course in a Marketing Curricula.

    ERIC Educational Resources Information Center

    Judd, Vaughan C.

    This paper argues that professional selling within the context of a marketing curricula in a business school should be described and practiced in a manner compatible with the marketing concept, which emphasizes satisfaction of consumers' needs. The paper looks at textbook approaches to sales presentations to determine their congruency with the…

  20. Best-Selling Books Advising Parents about Gender: A Feminist Analysis

    ERIC Educational Resources Information Center

    Krafchick, Jennifer L.; Schindler Zimmerman, Toni; Haddock, Shelley A.; Banning, James H.

    2005-01-01

    To determine how gender in parenting was addressed, a content analysis was conducted on the six best-selling self-help books (1997-2002) that provide general parenting advice. A feminist perspective was used to code gendered meaning units in the six books. Findings were that 82% of the implicit gender messages across all books were stereotypical,…

  1. 7 CFR 29.9406 - Failure of warehouse to comply with opening and selling schedule.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 2 2010-01-01 2010-01-01 false Failure of warehouse to comply with opening and... Markets § 29.9406 Failure of warehouse to comply with opening and selling schedule. (a) Each warehouse... warehouse may exceed its scheduled sales opportunity for designated or undesignated tobacco, but the...

  2. 9 CFR 201.56 - Market agencies selling on commission; purchases from consignment.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    .... (c) Key employees not to purchase livestock out of consignments. No market agency engaged in selling... consignment for any purpose for their own account, either directly or indirectly. (d) Purchase from... the account of sale the name of the buyer and the nature of the relationship existing between...

  3. Marketing and Selling CD-ROM Products on the World-Wide Web.

    ERIC Educational Resources Information Center

    Walker, Becki

    1995-01-01

    Describes three companies' approaches to marketing and selling CD-ROM products on the World Wide Web. Benefits include low overhead for Internet-based sales, allowance for creativity, and ability to let customers preview products online. Discusses advertising, information delivery, content, information services, and security. (AEF)

  4. 9 CFR 205.104 - Registration of buyer, commission merchant, or selling agent-minimum information.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Registration of buyer, commission... AGRICULTURE CLEAR TITLE-PROTECTION FOR PURCHASERS OF FARM PRODUCTS Regulations § 205.104 Registration of buyer... registration of a buyer, commission merchant, or selling agent is as follows: (1) Buyer, commission...

  5. 9 CFR 205.104 - Registration of buyer, commission merchant, or selling agent-minimum information.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 9 Animals and Animal Products 2 2013-01-01 2013-01-01 false Registration of buyer, commission... AGRICULTURE CLEAR TITLE-PROTECTION FOR PURCHASERS OF FARM PRODUCTS Regulations § 205.104 Registration of buyer... registration of a buyer, commission merchant, or selling agent is as follows: (1) Buyer, commission...

  6. 9 CFR 205.104 - Registration of buyer, commission merchant, or selling agent-minimum information.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 9 Animals and Animal Products 2 2012-01-01 2012-01-01 false Registration of buyer, commission... AGRICULTURE CLEAR TITLE-PROTECTION FOR PURCHASERS OF FARM PRODUCTS Regulations § 205.104 Registration of buyer... registration of a buyer, commission merchant, or selling agent is as follows: (1) Buyer, commission...

  7. 9 CFR 205.104 - Registration of buyer, commission merchant, or selling agent-minimum information.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 9 Animals and Animal Products 2 2014-01-01 2014-01-01 false Registration of buyer, commission... AGRICULTURE CLEAR TITLE-PROTECTION FOR PURCHASERS OF FARM PRODUCTS Regulations § 205.104 Registration of buyer... registration of a buyer, commission merchant, or selling agent is as follows: (1) Buyer, commission...

  8. 9 CFR 205.104 - Registration of buyer, commission merchant, or selling agent-minimum information.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 9 Animals and Animal Products 2 2011-01-01 2011-01-01 false Registration of buyer, commission... AGRICULTURE CLEAR TITLE-PROTECTION FOR PURCHASERS OF FARM PRODUCTS Regulations § 205.104 Registration of buyer... registration of a buyer, commission merchant, or selling agent is as follows: (1) Buyer, commission...

  9. Selling Addiction: A Workshop Kit on Tobacco and Alcohol Advertising. A Media Literacy Workshop Kit.

    ERIC Educational Resources Information Center

    Barnes, Mary Ellen; And Others

    This kit consists of: (1) a leader's guide; (2) an 18-minute videotape containing three 6-minute discussion starter segments analyzing typical commercials and advertising techniques; (3) a special issue of "Media Values" magazine on the theme "Fatal Attraction: The Selling of Addiction"; (4) an 8-page booklet "Awareness to Action: Media Literacy…

  10. Writing Juvenile Stories and Novels: How to Write and Sell Fiction for Young People.

    ERIC Educational Resources Information Center

    Whitney, Phyllis A.

    Phyllis Whitney, author of more than 30 juvenile novels and 20 adult novels, offers specific advice to aspiring writers on how to write, and sell, fiction for young people. Telling a good story, entertaining the reader, is the most important factor in each of the four divisions of children's books. These divisions can be structured roughly…

  11. 18 CFR 292.312 - Termination of obligation to sell to qualifying facilities.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 18 Conservation of Power and Water Resources 1 2010-04-01 2010-04-01 false Termination of... Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY REGULATIONS UNDER THE PUBLIC UTILITY...) Competing retail electric suppliers are willing and able to sell and deliver electric energy to...

  12. Health-seeking behaviour of women selling sex in Lahore, Pakistan.

    PubMed

    Khan, M S; Unemo, M; Zaman, S; Lundborg, C Stålsby

    2011-07-01

    The aim of this study was to describe the knowledge and health-seeking behaviour related to sexually transmitted infections (STIs) and abortion among women selling sex in Lahore, Pakistan. This was a cross-sectional, community-based, quantitative study. A total of 730 women selling sex were recruited by respondent-driven sampling. A pretested structured questionnaire was administered through face-to-face interviews. The median age of the participants was 30 years. Thirteen percent of the participants said it was common for them to have an abnormal vaginal discharge. Seventy-five percent of the participants recognized STIs as either leucorrhoea or AIDS. Sixty-five percent of the participants complained of having suffered from STI(s) in the six months preceding the survey, of whom 28% sought treatment. Women selling sex who reported consistent condom use were 1.5 times (95% confidence interval [CI]: 1.1-2.2) more likely to seek treatment than women who did not report consistent condom use. The level of knowledge about STIs remains low among women selling sex in Lahore, Pakistan, and health-seeking behaviour for the management of STIs and abortions is influenced by ability to pay and ease of access in the private sector.

  13. Selling the "Electrical Dream" in the 1920s: A Case Study in the Manipulation of Consciousness.

    ERIC Educational Resources Information Center

    Feldman, Andrew

    To illustrate the relevance of critical communication history and theory to media ethics, this paper examines a crucial episode in the history of public relations: the American electrical industry's cooperative advertising and public relations efforts in the 1920s to sell the "electrical idea" to consumers. The paper first enumerates…

  14. 9 CFR 201.56 - Market agencies selling on commission; purchases from consignment.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 9 Animals and Animal Products 2 2013-01-01 2013-01-01 false Market agencies selling on commission; purchases from consignment. 201.56 Section 201.56 Animals and Animal Products GRAIN INSPECTION, PACKERS AND.... (c) Key employees not to purchase livestock out of consignments. No market agency engaged in...

  15. Standard and Poor's How to Invest. A Handbook for Buying and Selling Stocks and Bonds.

    ERIC Educational Resources Information Center

    Standard & Poor's Corp., New York, NY.

    Designed for the general public and possibly suitable also for high school and college economic students, the handbook gives general information about investing in the stock market. Arranged into seven sections, the handbook discusses the typical shareholder, common and preferred stocks, buying and selling bonds, how to develop a personal…

  16. Effects of MeJA on Arabidopsis metabolome under endogenous JA deficiency

    PubMed Central

    Cao, Jingjing; Li, Mengya; Chen, Jian; Liu, Pei; Li, Zhen

    2016-01-01

    Jasmonates (JAs) play important roles in plant growth, development and defense. Comprehensive metabolomics profiling of plants under JA treatment provides insights into the interaction and regulation network of plant hormones. Here we applied high resolution mass spectrometry based metabolomics approach on Arabidopsis wild type and JA synthesis deficiency mutant opr3. The effects of exogenous MeJA treatment on the metabolites of opr3 were investigated. More than 10000 ion signals were detected and more than 2000 signals showed significant variation in different genotypes and treatment groups. Multivariate statistic analyses (PCA and PLS-DA) were performed and a differential compound library containing 174 metabolites with high resolution precursor ion-product ions pairs was obtained. Classification and pathway analysis of 109 identified compounds in this library showed that glucosinolates and tryptophan metabolism, amino acids and small peptides metabolism, lipid metabolism, especially fatty acyls metabolism, were impacted by endogenous JA deficiency and exogenous MeJA treatment. These results were further verified by quantitative reverse transcription PCR (RT-qPCR) analysis of 21 related genes involved in the metabolism of glucosinolates, tryptophan and α-linolenic acid pathways. The results would greatly enhance our understanding of the biological functions of JA. PMID:27883040

  17. Effects of MeJA on Arabidopsis metabolome under endogenous JA deficiency

    NASA Astrophysics Data System (ADS)

    Cao, Jingjing; Li, Mengya; Chen, Jian; Liu, Pei; Li, Zhen

    2016-11-01

    Jasmonates (JAs) play important roles in plant growth, development and defense. Comprehensive metabolomics profiling of plants under JA treatment provides insights into the interaction and regulation network of plant hormones. Here we applied high resolution mass spectrometry based metabolomics approach on Arabidopsis wild type and JA synthesis deficiency mutant opr3. The effects of exogenous MeJA treatment on the metabolites of opr3 were investigated. More than 10000 ion signals were detected and more than 2000 signals showed significant variation in different genotypes and treatment groups. Multivariate statistic analyses (PCA and PLS-DA) were performed and a differential compound library containing 174 metabolites with high resolution precursor ion-product ions pairs was obtained. Classification and pathway analysis of 109 identified compounds in this library showed that glucosinolates and tryptophan metabolism, amino acids and small peptides metabolism, lipid metabolism, especially fatty acyls metabolism, were impacted by endogenous JA deficiency and exogenous MeJA treatment. These results were further verified by quantitative reverse transcription PCR (RT-qPCR) analysis of 21 related genes involved in the metabolism of glucosinolates, tryptophan and α-linolenic acid pathways. The results would greatly enhance our understanding of the biological functions of JA.

  18. Nanoporous Silicon Ignition of JA2 Propellant

    DTIC Science & Technology

    2014-06-01

    2 Figure 2. Photograph of the activated nanoporous silicon chip in the PVC container showing attached firing leads; JA2 propellant disk rests...chips, and each chip was placed singly in a transparent, flexible polyvinyl chloride ( PVC ) container. The PVC container (see figure 2) contained...room. The electrical leads on the outside of the PVC container were connected to the firing circuitry (an impressed voltage of 3 V across the chip

  19. Organ markets and human dignity: on selling your body and soul.

    PubMed

    Stempsey, W E

    2000-08-01

    This article addresses the ethics of selling transplantable organs. I examine and refute the claim that Catholic teaching would permit and even encourage an organ market. The acceptance of organ transplantation by the Church and even its praise of organ donors should not distract us from the quite explicit Church teaching that condemns an organ market. I offer some reasons why the Church should continue to disapprove of an organ market. The recent commercial turn in medicine can blind us to the problem of an organ market. In addition, the reliance on the gift image in organ transplantation raises difficulties of its own. What is needed is a fuller appreciation of the fact that the human person is essentially embodied with all its parts, and not merely an autonomous being that possesses organs as properties to sell. I support this vision of the embodied human person by appealing to the writings of Immanuel Kant.

  20. Memorandum on ground-water investigations in the Sells area, Papago Indian Reservation, Pima County, Arizona

    USGS Publications Warehouse

    Coates, D.R.

    1954-01-01

    From 1950 to the present date the Ground Water Branch, U.S. Geological Survey, has been collecting data about the ground-water supply in the Sells area, at the request of the Bureau of Indian Affairs, Papago Indian Agency.  The purpose of these studies has been to aid in locating and developing additional ground-water supplies for the community of Sells, the agency headquarters.  The work has been financed by and has been in cooperation with the Papago Indian Agency.  In addition to the author of this memorandum, the following personnel aided in collecting data: D. G. Metzger, H. E. Skibitzke, S.F. Turner, H. N. Wolcott, and C. B. Yost, Jr.

  1. Subscriptions to share: new way to sell content or help fundraising online.

    PubMed

    James, John S

    2003-11-28

    While exploring business models for the newsletter we found a radically different way to sell information or help raise funds online. A system designed to encourage sharing allows subscribers or donors to use parts of their subscription to create new subscriptions for others, without the publisher's involvement--eliminating registration, reducing transaction costs, encouraging larger subscriptions or donations, and creating opportunities for bringing people together around an idea, purpose, or cause.

  2. An overview of the key routes to the best selling 5-membered ring heterocyclic pharmaceuticals

    PubMed Central

    2011-01-01

    Summary This review presents a comprehensive overview on selected synthetic routes towards commercial drug compounds as published in both journal and patent literature. Owing to the vast number of potential structures, we have concentrated only on those drugs containing five-membered heterocycles and focused principally on the assembly of the heterocyclic core. In order to target the most representative chemical entities the examples discussed have been selected from the top 200 best selling drugs of recent years. PMID:21647262

  3. Children's understanding of the selling versus persuasive intent of junk food advertising: implications for regulation.

    PubMed

    Carter, Owen B J; Patterson, Lisa J; Donovan, Robert J; Ewing, Michael T; Roberts, Clare M

    2011-03-01

    Evidence suggests that until 8 years of age most children are cognitively incapable of appreciating the commercial purpose of television advertising and are particularly vulnerable to its persuasive techniques. After this age most children begin to describe the 'selling' intent of advertising and it is widely assumed this equips them with sufficient cognitive defences to protect against advertisers' persuasion attempts. However, much of the previous literature has been criticised for failing to differentiate between children's awareness of 'selling' versus 'persuasive' intent, the latter representing a more sophisticated understanding and superior cognitive defence. Unfortunately there is little literature to suggest at what age awareness of 'persuasive intent' emerges; our aim was to address this important issue. Children (n = 594) were recruited from each grade from Pre-primary (4-5 years) to Grade 7 (11-12 years) from ten primary schools in Perth, Western Australia and exposed to a McDonald's television advertisement. Understanding the purpose of television advertising was assessed both nonverbally (picture indication) and verbally (small discussion groups of 3-4), with particular distinction made between selling versus persuasive intent. Consistent with previous literature, a majority of children described the 'selling' intent of television advertising by 7-8 years both nonverbally and verbally, increasing to 90% by 11-12 years. Awareness of 'persuasive' intent emerged slowly as a function of age but even by our oldest age-group was only 40%. Vulnerability to television advertising may persist until children are far older than previously thought. These findings have important implications regarding the debate surrounding regulation of junk food (and other) advertising aimed at children.

  4. The Stock Price Prediction and Sell-buy Strategy Model by Genetic Network Programming

    NASA Astrophysics Data System (ADS)

    Mori, Shigeo; Hirasawa, Kotaro; Hu, Jinglu

    Various stock prices predicting and sell-buy strategy models have been so far proposed. They are classified as the fundamental analysis using the achievements of the companies and the trend of business, etc., and the technical analysis which carries out the numerical analysis of the movement of stock prices. On the other hand, as one of the methods for data mining which finds out the regularity from a vast quantity of stock price data, Genetic Algorithm (GA) has been so far applied widely. As a concrete example, the optimal values of parameters of stock indices like various moving averages and rates of deviation, etc. is computed by GA, and there have been developed various methods for predicting stock prices and determinig sell-buy strategy based on it. However, it is hard to determine which is the most effective index by the conventional GA. Moreover, the most effective one depends on the brands. So in this paper, a stock price prediction and sell-buy strategy model which searches for the optimal combination of various indices in the technical analysis has been proposed using Genetic Network programming and its effectiveness is confirmed by simulations.

  5. Gun Carrying and Drug Selling Among Young Incarcerated Men and Women

    PubMed Central

    Hemenway, David

    2006-01-01

    This paper examines the relationship between illegal drug economy involvement, gun-related victimization, and recent gun carrying among young men and women incarcerated in a state prison in the United States. Interviews were conducted with 18- to 25-year old incarcerated men (n = 135) and women (n = 69) between July 1999 and October 2000. Forty-five percent of men and 16% of women reported carrying a gun in the year prior to incarceration. Respondents who sold crack cocaine or other drugs were more likely to have carried guns than those not selling drugs. However, hard drug use was not associated with gun carrying among men. All ten women who carried guns had used hard drugs. Sixty-seven percent of men and 28% of women had been shot at. Respondents who sold crack cocaine were at elevated risk of being shot at. Among men, selling crack (OR = 10.2, 95% CI = 2.5, 42.1) and ever being shot at (OR = 4.6, 95% CI = 1.7, 12.2), were associated with carrying guns. These findings provide further evidence of a link between crack selling (but not necessarily drug using) and gun carrying. PMID:16736375

  6. 40 CFR 1051.330 - May I sell vehicles from an engine family with a suspended certificate of conformity?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR POLLUTION CONTROLS CONTROL OF EMISSIONS FROM RECREATIONAL ENGINES AND VEHICLES Testing Production-Line Vehicles and Engines § 1051.330 May I sell vehicles from...

  7. 40 CFR 1048.330 - May I sell engines from an engine family with a suspended certificate of conformity?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR POLLUTION CONTROLS CONTROL OF EMISSIONS FROM NEW, LARGE NONROAD SPARK-IGNITION ENGINES Testing Production-line Engines § 1048.330 May I sell engines from an...

  8. 40 CFR 1054.330 - May I sell engines from an engine family with a suspended certificate of conformity?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR POLLUTION CONTROLS CONTROL OF EMISSIONS FROM NEW, SMALL NONROAD SPARK-IGNITION ENGINES AND EQUIPMENT Production-line Testing § 1054.330 May I sell engines from...

  9. Selling Shakespeare.

    ERIC Educational Resources Information Center

    Milburn, Michael

    2002-01-01

    Considers how the successful teaching of Shakespeare represents the ultimate challenge for many English teachers, one that measures their ability to enlighten and inspire. Discusses how her colleague approaches teaching Shakespeare to eight graders. Describes how she approaches teaching Henry V to her ninth grade students. (SG)

  10. Selling hospice.

    PubMed

    Halabi, Sam

    2014-01-01

    Americans are increasingly turning to hospice services to provide them with medical care, pain management, and emotional support at the end of life. The increase in the rates of hospice utilization is explained by a number of factors including a "hospice movement" dating to the 1970s which emphasized hospice as a tool to promote dignity for the terminally ill; coverage of hospice services by Medicare beginning in 1983; and, the market for hospice services provision, sustained almost entirely by governmental reimbursement. On the one hand, the growing acceptance of hospice may be seen as a sign of trends giving substance to the death-with-dignity movement and the growing strength of end-of-life decision-makers and planners who integrate medical, community, family and spiritual networks. On the other hand, the precise relationship between the death-with-dignity and commercial processes driving hospice utilization rates are not well understood. On May 2, 2013, the U.S. Government intervened in a lawsuit brought by former hospice employees alleging that behind Vitas Innovative Hospice Care, the largest for-profit hospice service provider in the United States, lie an intricate web of incentives for patient intake nurses, physicians and marketers which not only drove hospice patients to use more expensive (and medically unnecessary) crisis care services, but influenced patient and family decisions as to whether or not to discontinue curative treatment. The corporate, investment, and regulatory history behind Vitas provides an important insight into the market realities behind Americans' embrace of hospice care and the risks to patient autonomy and health that accompany the commercialization of this ethically and morally complex health care service.

  11. STEM Sell

    ERIC Educational Resources Information Center

    Pantic, Zorica

    2007-01-01

    Between 1994 and 2003, employment in science, technology, engineering and math (STEM) fields grew by a remarkable 23 percent, compared with 17 percent in non-STEM fields, according to federal data. The Bureau of Labor Statistics predicts continued strong growth in STEM job openings through 2014, with emphasis on life sciences, environmental…

  12. STEM Sell

    ERIC Educational Resources Information Center

    Ullman, Ellen

    2012-01-01

    In his State of the Union Address in January, President Obama said he visited "many business leaders who want to hire in the United States but can't find workers with the right skills." Not hard to imagine, considering the U.S. Department of Commerce reports that only one in every 18 workers in America currently works in a science, technology,…

  13. Effect of corn stover compositional variability on minimum ethanol selling price (MESP).

    PubMed

    Tao, Ling; Templeton, David W; Humbird, David; Aden, Andy

    2013-07-01

    A techno-economic sensitivity analysis was performed using a National Renewable Energy Laboratory (NREL) 2011 biochemical conversion design model varying feedstock compositions. A total of 496 feedstock near infrared (NIR) compositions from 47 locations in eight US Corn Belt states were used as the inputs to calculate minimum ethanol selling price (MESP), ethanol yield (gallons per dry ton biomass feedstock), ethanol annual production, as well as total installed project cost for each composition. From this study, the calculated MESP is $2.20 ± 0.21 (average ± 3 SD) per gallon ethanol.

  14. University of Washington - AFA symposium: shall we sell the nation's forest lands

    SciTech Connect

    Lewis, M.B.

    1983-07-01

    Debate over whether federal forest land should be sold to private owners has been emotional and confused. Congress has received a variety of interpretations of the administration's intent that range from slight modifications in existing programs to virtual privatization. Advocates of the asset management program point out that its intent is to sell only excess property with a higher private use, to make land management more efficient, and to help reduce the national debt. Opponents argue that it violates the Endangered Species and the National Environmental Protection Acts. They disagree on how the public interest is best served. The article presents the arguments on both sides of the issue. (DCK)

  15. Contractor Sales Training: Providing the Skills Necessary to Sell Comprehensive Home Energy Upgrades

    SciTech Connect

    Billingsley, Megan; Stuart, Elizabeth

    2011-08-17

    Many comprehensive home energy efficiency programs rely on contractors as the customer-facing ‘front line’ to sell energy improvements. Adding sales skills to contractors’ existing technical expertise is key to converting more assessments into comprehensive home energy upgrades. Leading programs recognize the need to support contractors with sales and business training to help them succeed and to support the growth of the home performance industry for the long term. A number of contractor sales training efforts are emerging, including some programs that are seeing encouraging early results.

  16. Selling the Space Telescope - The interpenetration of science, technology, and politics

    NASA Technical Reports Server (NTRS)

    Smith, Robert W.

    1991-01-01

    Attention is given to the politics of initiating the Space Telescope program and to the manner in which the coalition, or working consensus, for the Telescope was assembled, in particular, the role played by astronomers. It is contended that what ensued was a case study in the influence of government patronage on a large-scale scientific and technological program. It is concluded that while a politically feasible Space Telescope did result, in the selling process the Telescope had been both oversold and underfunded.

  17. Endogenous Bioactive Jasmonate Is Composed of a Set of (+)-7-iso-JA-Amino Acid Conjugates.

    PubMed

    Yan, Jianbin; Li, Suhua; Gu, Min; Yao, Ruifeng; Li, Yuwen; Chen, Juan; Yang, Mai; Tong, Jianhua; Xiao, Langtao; Nan, Fajun; Xie, Daoxin

    2016-12-01

    Jasmonates (JAs) regulate a wide range of plant defense and development processes. The bioactive JA is perceived by its receptor COI1 to trigger the degradation of JASMONATE ZIM-DOMAIN (JAZ) proteins and subsequently derepress the JAZ-repressed transcription factors for activation of expression of JA-responsive genes. So far, (+)-7-iso-JA-l-Ile has been the only identified endogenous bioactive JA molecule. Here, we designed coronafacic acid (CFA) conjugates with all the amino acids (CFA-AA) to mimic the JA amino acid conjugates, and revealed that (+)-7-iso-JA-Leu, (+)-7-iso-JA-Val, (+)-7-iso-JA-Met, and (+)-7-iso-JA-Ala are new endogenous bioactive JA molecules. Furthermore, our studies uncover the general characteristics for all the bioactive JA molecules, and provide a new strategy to synthetically generate novel active JA molecules.

  18. Selling space colonization and immortality: A psychosocial, anthropological critique of the rush to colonize Mars

    NASA Astrophysics Data System (ADS)

    Slobodian, Rayna Elizabeth

    2015-08-01

    Extensive media coverage regarding the proposal to send four people to Mars by 2025 has exploded recently. Private enterprise has taken the reins to venture into space, which has typically only been reserved for government agencies. I argue, that with this new direction comes less regulation, raising questions regarding the ethics of sending people into outer space to colonize Mars within a decade. Marketers selling colonization to the public include perspectives such as biological drives, species survival, inclusiveness and utopian ideals. I challenge these narratives by suggesting that much of our desire to colonize space within the next decade is motivated by ego, money and romanticism. More specifically, I will examine the roles that fear and stories of immortality play within selling space and how those stories are marketed. I am passionate about space and hope that one day humanity will colonize other worlds, but the rush to settle is dangerous and careless. I assert that humanity should first gain more experience and knowledge before colonizing outer space, using this research to mitigate the risk to astronauts and proceed with careful consideration for the lives of potential astronauts.

  19. “GETTING HIGH AND GETTING BY”: DIMENSIONS OF DRUG SELLING BEHAVIORS AMONG AMERICAN MEXICAN GANG MEMBERS IN SOUTH TEXAS

    PubMed Central

    Valdez, Avelardo; Sifaneck, Stephen J.

    2010-01-01

    This article discerns the role that Mexican American gang members play in drug markets, and the relationship between gang members’drug use and drug selling in South Texas. A four-part typology based on the two dimensions of gang type and gang member emerged from this qualitative analysis of 160 male gang members: Homeboys, Hustlers, Slangers, and Ballers. Major findings include the following: (1) many gang members are user/sellers and are not profit-oriented dealers, (2) gangs commonly do extend “protection” to drug-selling members, and (3) proximity to Mexican drug markets, adult prison gangs, and criminal family members may play important roles in whether these gang members have access and the profit potential to actually deal drugs. This research contributes to our complex intersections between gangs, drug using, and drug selling. PMID:21218191

  20. Structural Factors Influencing Patterns of Drug Selling and Use and HIV Risk in the San Salvador Metropolitan Area

    PubMed Central

    Dickson-Gomez, Julia

    2013-01-01

    This article explores differences in the social context in which crack sales and use and HIV risk take place in seven low-income communities in San Salvador, and structural factors that may influence these differences. The organization of drug selling varied among the communities on a number of dimensions including: whether drug sales were open or closed systems; the type of drug-selling site; and the participation of drug users in drug-distribution roles. Drug-use sites also varied according to whether crack was used in private, semiprivate, or public spaces, and whether individuals used drugs alone or with other drug users. Three patterns of drug use and selling were identified based on the dimensions outlined above. Structural factors that influenced these patterns included the geographic location of the communities, their physical layout, gang involvement in drug sales, and police surveillance. Implications for HIV risk and prevention are explored for each pattern. PMID:20550091

  1. To give or sell human gametes--the interplay between pragmatics, policy and ethics.

    PubMed

    Daniels, K R

    2000-06-01

    The ever-growing acceptance and use of assisted human reproduction techniques has caused demand for "donated" sperm and eggs to outstrip supply. Medical professionals and others argue that monetary reward is the only way to recruit sufficient numbers of "donors". Is this a clash between pragmatics and policy/ethics? Where monetary payments are the norm, alternative recruitment strategies used successfully elsewhere may not have been considered, nor the negative consequences of commercialism on all participants thought through. Considerations leading some countries to ban the buying and selling of sperm, eggs and embryos are outlined and a case made that the collective welfare of all involved parties be the primary consideration in this, at times heated, debate.

  2. 'No willpower required'. The slimming industry and the right to sell dreams.

    PubMed

    de Beaufort, Inez; Vandamme, Sofie

    2008-03-01

    Many products to lose weight are presented on the market. The products are advertised with two 'arguments': the health-argument: obesity and overweight are dangerous for your health, and the beauty argument: obesity and overweight make you ugly. The authors raise ethical questions with regard to the promises made and discuss the following arguments: the exploitation of vulnerable persons who are told they are unhealthy and ugly, the stigmatization of obese people as lazy and without willpower, and the harm some treatments may cause. They argue that raising the health argument should be analyzed very critically as obese people are vulnerable and susceptible to all kind of promises. With regard to the beauty argument, they argue that 'selling dreams' is perhaps less problematic as people are not as susceptible and vulnerable with regard to promises on beauty and therefore less likely to be seduced by such promises. People should be empowered to be very critical of (advertisements for) slimming products.

  3. Process and dynamics of traditional selling wild edible mushrooms in tropical Mexico

    PubMed Central

    Ruán-Soto, Felipe; Garibay-Orijel, Roberto; Cifuentes, Joaquín

    2006-01-01

    Background More than twelve temperate-inhabitant Mexican ethnic groups are considered to be mycophilic and to have extensive traditional mycological knowledge. In contrast, inhabitants of tropical lands have been studied only superficially and their mycological knowledge is less well known. In this paper, we report the results of an ethnomycological research in markets of a wide area of the Mexican tropics. Our aims were to describe the dynamics related to the traditional selling process of wild mushrooms and to determine the tendencies of informants toward mushrooms (mycophily vs. mycophoby). Methods We visited 25 markets of 12 different settlements in the states of Oaxaca, Tabasco and Veracruz and collected information by participant observation as well as by 291 non-structured and semi-structured interviews. Results Mushroom selling was observed in four towns in Oaxaca and in two in Tabasco. Women represented 81.82% of sellers, while indigenous people (Chinantecos, Chontales, Ch'oles and Zoques) comprised 68.18%. Mushroom commercialization took place in secondary mobile markets and only in peasant stands. Mushroom collectors gather the resource in places with secondary vegetation, farmed areas and cattle fields. Because of land tenure restrictions mushroom sellers did not normally collect mushrooms themselves. In Oaxaca, we observed economic dynamics not based on capitalism, such as exchange, reciprocity and barter. Conclusion The sale of some wild edible mushrooms, the large amounts of commercialization of Schizophyllum commune, the complicated intermediary process, as well as the insertion of mushrooms into different informal economic practices are all evidence of an existent mycophily in a sector of the population of this region of the Mexican tropics. Among our informants, urban mestizo people were mycophobic, rural mestizo people were non-mycophilic and indigenous people were true mycophilic. PMID:16393345

  4. Selling androgenic anabolic steroids by the pound: identification and analysis of popular websites on the Internet.

    PubMed

    Cordaro, F G; Lombardo, S; Cosentino, M

    2011-12-01

    Internet websites offering androgenic anabolic steroids (AAS) were identified and available products were examined. Keywords for the website search were: "anabolic steroids," "anabolic steroids buy," "anabolic steroid purchase." The first 10 websites offering AAS in the first 10 pages of results were considered. At least two AAS-containing products per website were selected. Thirty AAS-selling websites were identified, mainly located in the United States (46.7%) and Europe (30%). Most websites sold other anabolic/ergogenic products (clenbuterol, 76.7%; GH/IGF, 60.0%; thyroid hormones, 46.7%; erythropoietin, 30.0%; insulin, 20.0%) or products for AAS-related adverse effects (mainly: estrogen antagonists, 63.3%; products for erectile dysfunction, 56.7%; 5α-reductase inhibitors, 33.3%; anti-acne products, 33.3%). AAS were sold as medicines (69.6%) or as dietary supplements (30.4%). AAS in medicines were mainly: nandronole (20.4%), methandrostenolone (18.4%), and testosterone (12.2%). Dietary supplements contained mainly DHEA and included several fake compounds. Manufacturers were declared for 97.9% of medicines and 66.7% of dietary supplements; however, several manufacturers were not found on the Internet. Described benefits were usually few adverse effects and no estrogenicity. Toxicity was seldom reported and presented as mild. Recommended doses were two-fourfold higher than current medical recommendations. In conclusion, misleading information and deceiving practices were common findings on AAS-selling websites, indicating their deleterious potential for public health.

  5. Heterosexual behaviours among men who sell sex to men in coastal Kenya

    PubMed Central

    Smith, Adrian D.; Muhaari, Allan D.; Agwanda, Carole; Kowuor, Dickens; van der Elst, Elise; Davies, Alun; Graham, Susan M.; Jaffe, Harold W.; Sanders, Eduard J.

    2016-01-01

    Objective African men who have sex with men often sell sex to men, and MSM who sell sex (MSM-SW) often also have female partners. We compared sexual risk behaviour of MSM-SW who were sexually active with female partners (bisexual MSW) to MSM-SW with only male partners (exclusive MSW). Design Descriptive behavioural study Methods A novel, validated daily event and partner diary self-completed by 82 MSM who sold sex over a follow-up period of 42 days with weekly review. Cumulative individual counts of sex and condomless sex were compiled by partner characteristics. The incidence of specific partnerships and sex acts were compared within and between bisexual and exclusive MSW. Results Most (59%) MSM-SW reported female partners during follow-up. The majority of both male and female partners were cash-paying clients originating locally. Bisexual MSW reported a similar rate of condomless sex with male and female partners, but significantly fewer male partners than exclusive MSW. Bisexual MSW had lower HIV prevalence, were more likely to only report insertive anal sex roles, and reported lower frequencies of condomless receptive anal sex than exclusive MSW. Conclusion Bisexually active male sex workers in coastal Kenya create HIV and other sexually transmitted infection transmission pathways to partners and clients in both MSM and heterosexual networks, but differed from exclusive MSW in having lower HIV acquisition and transmission risks. Epidemiological projection methods are liable to overestimate bridging potential of MSM-SW and MSM populations without account for systematic differences in risk within these populations. PMID:26565965

  6. 41 CFR 102-74.75 - May Federal agencies sell tobacco products in vending machines in Government-owned and leased space?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... sell tobacco products in vending machines in Government-owned and leased space? 102-74.75 Section 102... Services § 102-74.75 May Federal agencies sell tobacco products in vending machines in Government-owned and... machines in Government-owned and leased space. The Administrator of GSA or the head of an Agency...

  7. 41 CFR 102-74.75 - May Federal agencies sell tobacco products in vending machines in Government-owned and leased space?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... sell tobacco products in vending machines in Government-owned and leased space? 102-74.75 Section 102... Services § 102-74.75 May Federal agencies sell tobacco products in vending machines in Government-owned and... machines in Government-owned and leased space. The Administrator of GSA or the head of an Agency...

  8. 41 CFR 102-74.75 - May Federal agencies sell tobacco products in vending machines in Government-owned and leased space?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... sell tobacco products in vending machines in Government-owned and leased space? 102-74.75 Section 102... Services § 102-74.75 May Federal agencies sell tobacco products in vending machines in Government-owned and... machines in Government-owned and leased space. The Administrator of GSA or the head of an Agency...

  9. 41 CFR 102-74.75 - May Federal agencies sell tobacco products in vending machines in Government-owned and leased space?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... sell tobacco products in vending machines in Government-owned and leased space? 102-74.75 Section 102... Services § 102-74.75 May Federal agencies sell tobacco products in vending machines in Government-owned and... machines in Government-owned and leased space. The Administrator of GSA or the head of an Agency...

  10. 41 CFR 102-74.75 - May Federal agencies sell tobacco products in vending machines in Government-owned and leased space?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... sell tobacco products in vending machines in Government-owned and leased space? 102-74.75 Section 102... Services § 102-74.75 May Federal agencies sell tobacco products in vending machines in Government-owned and... machines in Government-owned and leased space. The Administrator of GSA or the head of an Agency...

  11. 30 CFR 206.355 - How do I calculate royalty due on geothermal resources I sell at arm's length to a purchaser for...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 30 Mineral Resources 2 2010-07-01 2010-07-01 false How do I calculate royalty due on geothermal... Geothermal Resources § 206.355 How do I calculate royalty due on geothermal resources I sell at arm's length to a purchaser for direct use? If you sell geothermal resources produced from Class I, II, or...

  12. 30 CFR 1206.355 - How do I calculate royalty due on geothermal resources I sell at arm's length to a purchaser for...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 30 Mineral Resources 3 2011-07-01 2011-07-01 false How do I calculate royalty due on geothermal... Revenue PRODUCT VALUATION Geothermal Resources § 1206.355 How do I calculate royalty due on geothermal resources I sell at arm's length to a purchaser for direct use? If you sell geothermal resources...

  13. 41 CFR 302-11.308 - How much will I receive for reimbursement when I purchase or sell land in excess of what...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... site? 302-11.308 Section 302-11.308 Public Contracts and Property Management Federal Travel Regulation... reimbursement when I purchase or sell land in excess of what reasonably relates to the residence site? When you purchase or sell land in excess of what reasonably relates to the residence site, your reimbursement...

  14. 41 CFR 302-11.308 - How much will I receive for reimbursement when I purchase or sell land in excess of what...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... site? 302-11.308 Section 302-11.308 Public Contracts and Property Management Federal Travel Regulation... reimbursement when I purchase or sell land in excess of what reasonably relates to the residence site? When you purchase or sell land in excess of what reasonably relates to the residence site, your reimbursement...

  15. 17 CFR 301.300a - Form 300-A, for summary of buy-ins or sell-outs of all open contractual commitments.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 17 Commodity and Securities Exchanges 3 2010-04-01 2010-04-01 false Form 300-A, for summary of buy... Form 300-A, for summary of buy-ins or sell-outs of all open contractual commitments. This form shall be... summarize the buy-ins and sell-outs of those open contractual commitments and shall be accompanied by...

  16. 25 CFR 309.9 - When can non-Indians make and sell products in the style of Indian arts and crafts?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... of Indian arts and crafts? 309.9 Section 309.9 Indians INDIAN ARTS AND CRAFTS BOARD, DEPARTMENT OF THE INTERIOR PROTECTION OF INDIAN ARTS AND CRAFTS PRODUCTS § 309.9 When can non-Indians make and sell products in the style of Indian arts and crafts? A non-Indian can make and sell products in the style...

  17. 25 CFR 309.9 - When can non-Indians make and sell products in the style of Indian arts and crafts?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... of Indian arts and crafts? 309.9 Section 309.9 Indians INDIAN ARTS AND CRAFTS BOARD, DEPARTMENT OF THE INTERIOR PROTECTION OF INDIAN ARTS AND CRAFTS PRODUCTS § 309.9 When can non-Indians make and sell products in the style of Indian arts and crafts? A non-Indian can make and sell products in the style...

  18. 25 CFR 309.9 - When can non-Indians make and sell products in the style of Indian arts and crafts?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... of Indian arts and crafts? 309.9 Section 309.9 Indians INDIAN ARTS AND CRAFTS BOARD, DEPARTMENT OF THE INTERIOR PROTECTION OF INDIAN ARTS AND CRAFTS PRODUCTS § 309.9 When can non-Indians make and sell products in the style of Indian arts and crafts? A non-Indian can make and sell products in the style...

  19. 25 CFR 309.9 - When can non-Indians make and sell products in the style of Indian arts and crafts?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... of Indian arts and crafts? 309.9 Section 309.9 Indians INDIAN ARTS AND CRAFTS BOARD, DEPARTMENT OF THE INTERIOR PROTECTION OF INDIAN ARTS AND CRAFTS PRODUCTS § 309.9 When can non-Indians make and sell products in the style of Indian arts and crafts? A non-Indian can make and sell products in the style...

  20. 25 CFR 309.9 - When can non-Indians make and sell products in the style of Indian arts and crafts?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... of Indian arts and crafts? 309.9 Section 309.9 Indians INDIAN ARTS AND CRAFTS BOARD, DEPARTMENT OF THE INTERIOR PROTECTION OF INDIAN ARTS AND CRAFTS PRODUCTS § 309.9 When can non-Indians make and sell products in the style of Indian arts and crafts? A non-Indian can make and sell products in the style...

  1. 41 CFR 302-11.308 - How much will I receive for reimbursement when I purchase or sell land in excess of what...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... reimbursement when I purchase or sell land in excess of what reasonably relates to the residence site? When you purchase or sell land in excess of what reasonably relates to the residence site, your reimbursement will be limited to a pro rata reimbursement of the land reasonably related to the residence site....

  2. 41 CFR 302-11.308 - How much will I receive for reimbursement when I purchase or sell land in excess of what...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... reimbursement when I purchase or sell land in excess of what reasonably relates to the residence site? When you purchase or sell land in excess of what reasonably relates to the residence site, your reimbursement will be limited to a pro rata reimbursement of the land reasonably related to the residence site....

  3. RuBPCase activase mediates growth-defense tradeoffs: Silencing RCA redirects JA flux from JA-Ile to MeJA to attenuate induced defense responses in Nicotiana attenuata

    PubMed Central

    Mitra, Sirsha; Baldwin, Ian T.

    2014-01-01

    Summary RuBPCase activase (RCA), an abundant photosynthetic protein is strongly down-regulated in response to Manduca sexta’s oral secretion (OS) in Nicotiana attenuata. RCA-silenced plants are impaired not only in photosynthetic capacity and growth, but also in jasmonic acid (JA)-isoleucine (Ile) signaling, and herbivore resistance mediated by JA-Ile dependent defense traits. These responses are consistent with a resource-based growth-defense trade-off. Since JA+Ile-supplementation of OS restored WT levels of JA-Ile, defenses and resistance to M. sexta, but OS supplemented individually with JA- or Ile did not, the JA-Ile deficiency of RCA-silenced plants could not be attributed to lower JA or Ile pools or JAR4/6 conjugating activity. Similar levels of JA-Ile derivatives after OS elicitation indicated unaltered JA-Ile turnover and lower levels of other JA-conjugates ruled out competition from other conjugation reactions. RCA-silenced plants accumulated more methyl jasmonate (MeJA) after OS elicitation, which corresponded with increased jasmonate methyltransferase (JMT) activity. RCA-silencing phenocopies JMT over-expression, wherein elevated JMT activity redirects OS-elicited JA flux towards inactive MeJA, creating a JA sink which depletes JA-Ile and its associated defense responses. Hence RCA plays an additional non-photosynthetic role in attenuating JA-mediated defenses and their associated costs potentially allowing plants to anticipate resource-based constraints on growth before they actually occur. PMID:24491116

  4. Writing Treatments That Sell: How To Create and Market Your Story Ideas to the Motion Picture and TV Industry.

    ERIC Educational Resources Information Center

    Atchity, Kenneth; Wong, Chi-Li

    Pointing out that what sells a story idea for film or television is not writing the script but preparing the "treatment"--the brief pitch of a story to a producer or agent--this book furnishes dozens of examples of treatments from actual productions. The book makes the distinction between scripts designed for feature films, episodic…

  5. Gender Differences in Personal Selling Ethics Evaluations: Do They Exist and What Does Their Existence Mean for Teaching Sales Ethics?

    ERIC Educational Resources Information Center

    Donoho, Casey; Heinze, Timothy; Kondo, Christopher

    2012-01-01

    Sales career opportunities are growing, and the number of women in sales is increasing. Educators must adequately prepare both men and women for today's ethical sales dilemmas. Using the Personal Selling Ethics Scale, the current study analyzes the impact of idealism and relativism on the sales ethics evaluations of men and women. Results indicate…

  6. 40 CFR 1045.330 - May I sell engines from an engine family with a suspended certificate of conformity?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... family with a suspended certificate of conformity? 1045.330 Section 1045.330 Protection of Environment... engine family with a suspended certificate of conformity? You may sell engines that you produce after we suspend the engine family's certificate of conformity under § 1045.315 only if one of the following...

  7. 40 CFR 1042.330 - Selling engines from an engine family with a suspended certificate of conformity.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... with a suspended certificate of conformity. 1042.330 Section 1042.330 Protection of Environment... engines from an engine family with a suspended certificate of conformity. You may sell engines that you produce after we suspend the engine family's certificate of conformity under § 1042.315 only if one of...

  8. 40 CFR 1045.330 - May I sell engines from an engine family with a suspended certificate of conformity?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... family with a suspended certificate of conformity? 1045.330 Section 1045.330 Protection of Environment... engine family with a suspended certificate of conformity? You may sell engines that you produce after we suspend the engine family's certificate of conformity under § 1045.315 only if one of the following...

  9. 40 CFR 1042.330 - Selling engines from an engine family with a suspended certificate of conformity.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... with a suspended certificate of conformity. 1042.330 Section 1042.330 Protection of Environment... engines from an engine family with a suspended certificate of conformity. You may sell engines that you produce after we suspend the engine family's certificate of conformity under § 1042.315 only if one of...

  10. 40 CFR 1048.330 - May I sell engines from an engine family with a suspended certificate of conformity?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... family with a suspended certificate of conformity? 1048.330 Section 1048.330 Protection of Environment... family with a suspended certificate of conformity? You may sell engines that you produce after we suspend the engine family's certificate of conformity under § 1048.315 only if one of the following occurs:...

  11. Manifest Meanings: The Selling (Not Telling) of American Indian History and the Case of "The Black Horse Ledger"

    ERIC Educational Resources Information Center

    Gercken, Becca

    2010-01-01

    What is the value or perceived necessity--for an Indian or for a white man--of changing Northern Cheyenne history? How are a reader's conclusions affected by her perception of the race of the person altering that history? Why is it acceptable to sell but not tell American Indian history? An examination of the visual and discursive rhetoric of "The…

  12. California Court Upholds an Injunction Barring Community Colleges from Selling Books that Aren't Specifically Required for Courses.

    ERIC Educational Resources Information Center

    Fuchsberg, Gilbert

    1989-01-01

    Local bookstore owners charge that the selling of best-sellers and trade books unrelated to coursework by community college bookstores violates California's education code. The decision was not based on free-speech grounds but on the language of the state code. (MSE)

  13. Selling sex in a population-based study of high school seniors in sweden: demographic and psychosocial correlates.

    PubMed

    Svedin, Carl Göran; Priebe, Gisela

    2007-02-01

    Adolescents reporting selling sex for payment were studied with respect to socioeconomic background, perceived mental health and health behavior, antisocial behavior, sexual experiences, including sexual abuse and abusive behaviors, and the use of pornography. The sample consisted of a representative sample of 4,339 students (response rate, 77.2%) from the third year of upper secondary schools in five Swedish cities. Of the participating adolescents, 1.8% of the boys and 1.0% of the girls indicated that they had sold sex for money or other reimbursements. Selling sex was associated with having an immigrant background, higher level of unemployment in the family, and studying a practical/vocational program. Adolescents with the experience of selling sex had an increased risk for different psychosocial problems, such as poorer mental health, weekly use of alcohol, and antisocial behaviors. The adolescents selling sex were also characterized by having had a greater number of sexual experiences, a greater preoccupation with sex, relatively early sexual debut, and experience with sexual abuse as victim and/or perpetrator. The index group students watched pornography more frequently than the majority and they also watched more deviant forms of pornography. The significance of the higher frequency among boys and the gray zone between normal sexual exploration and prostitution are discussed.

  14. 41 CFR 102-34.105 - Before we sell a motor vehicle, what motor vehicle identification must we remove?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 41 Public Contracts and Property Management 3 2011-01-01 2011-01-01 false Before we sell a motor vehicle, what motor vehicle identification must we remove? 102-34.105 Section 102-34.105 Public Contracts... REGULATION PERSONAL PROPERTY 34-MOTOR VEHICLE MANAGEMENT Identifying and Registering Motor Vehicles...

  15. Teaching "Yes, And" … Improv in Sales Classes: Enhancing Student Adaptive Selling Skills, Sales Performance, and Teaching Evaluations

    ERIC Educational Resources Information Center

    Rocco, Richard A.; Whalen, D. Joel

    2014-01-01

    In an application of experiential learning, assessment, and career development, this article reports a field experiment of teaching sales students adaptive selling skills via an "Improvisational (Improv) Comedy" technique: "Yes, And." Students learn this well-established theatrical improv method via classroom lecture,…

  16. "Getting High and Getting By": Dimensions of Drug Selling Behaviors among American Mexican Gang Members in South Texas

    ERIC Educational Resources Information Center

    Valdez, Avelardo; Sifaneck, Stephen J.

    2004-01-01

    This article discerns the role that Mexican American gang members play in drug markets, and the relationship between gang members' drug use and drug selling in South Texas. A four-part typology based on the two dimensions of gang type and gang member emerged from this qualitative analysis of 160 male gang members: Homeboys, Hustlers, Slangers, and…

  17. Women who sell sex in a Ugandan trading town: life histories, survival strategies and risk.

    PubMed

    Gysels, Marjolein; Pool, Robert; Nnalusiba, Betty

    2002-01-01

    Little is known about the background of commercial sex workers in Africa. This study investigated how women in a trading town on the trans-Africa highway in southwest Uganda become involved in commercial sex work, which factors contribute to their economic success or lack of success, and what effect life trajectories and economic success have on negotiating power and risk behaviour. Over the course of two years detailed life histories of 34 women were collected through recording open, in-depth interviews, the collection of sexual and income and expenditure diaries, visits to the women's native villages, and participant observation. The women share similar disadvantaged backgrounds and this has played a role in their move into commercial sex. They have divergent experiences, however, in their utilisation of opportunities and in the level of success they achieve. They have developed different life styles and a variety of ways of dealing with sexual relationships. Three groups of women were identified: (1) women who work in the back-street bars, have no capital of their own and are almost entirely dependent on selling sex for their livelihood; (2) waitresses in the bars along the main road who engage in a more institutionalised kind of commercial sex, often mediated by middlemen and (3) the more successful entrepreneurs who earn money from their own bars as well as from commercial sex. The three groups had different risk profiles. Due partly to their financial independence from men, women in the latter group have taken control of sexual relationships and can negotiate good sexual deals for themselves, both financially and in terms of safe sex. The poorer women were more vulnerable and less able to negotiate safer sex. A disadvantaged background and restricted access to economic resources are the major reasons for women gravitating to commercial sex work. Various aspects of personality play a role in utilising income from commercial sex to set up an economic basis that

  18. Comparing the 2000 and 2005 factors affecting the selling price of feeder cattle sold at Arkansas livestock auctions.

    PubMed

    Troxel, T R; Barham, B L

    2007-12-01

    The objectives of the study were to determine how factors affecting the selling price of feeder calves changed from 2000 to 2005 and to examine the perception that discounts narrow or even disappear as calf supplies decrease and selling prices increase. Data from weekly Arkansas livestock auctions were collected from January 1 to December 31 in 2000 and 2005. Data included calf sex, breed type, color, muscle score, horn status, frame score, fill, condition, health, and BW. Mean selling prices for 2000 and 2005 were $92.91 +/- 15.05 and $118.32 +/- 15.13 (mean +/- SD; $/45.45 kg), respectively. Individual price observations were subtracted from the respective annual means and became the dependent variable. The selling prices for feeder calves sold in groups of 2 to 5 calves and in groups of >/= 6 calves were greater in 2005 than 2000 (P < 0.001). Steers received a greater premium ($6.48 +/- 0.09 vs. $6.02 +/- 0.08; mean +/- SE) and bull calves received greater discounts ($0.30 +/- 0.14 vs. $1.68 +/- 0.09) in 2005 than in 2000. Breeds types that increased in value from 2000 to 2005 were Angus x Hereford, Angus, Angus x Charolais, and Brahman (P < 0.001). Breed types that received a reduced selling price in 2005 compared with 2000 (P < 0.001) were one-fourth Brahman Cross, Charolais, Charolais x Limousin, Hereford x Limousin, Limousin, Limousin x one-fourth Brahman, Longhorn, Saler and Simmental. Yellow-white face, black-white face, black, and gray feeder calves received an increase in selling price from 2000 to 2005 (P < 0.001). Although fewer horned feeder calves were sold in 2005 (P < 0.01), they received greater discounts in 2005 than 2000 (-$2.86 +/- 0.16 and -$0.51 +/- 0.09; P < 0.001). In 2005, large-framed feeder calves did not receive the premium detected in 2000, but medium-framed feeder calves in 2005 received a greater selling price compared with 2000. Feeder calves with a muscle score of 1 received a greater premium in 2005 compared with 2000 ($2.58 +/- 0

  19. Sharing and selling of prescription medications in a college student sample

    PubMed Central

    Garnier, Laura M.; Arria, Amelia M.; Caldeira, Kimberly M.; Vincent, Kathryn B.; O’Grady, Kevin E.; Wish, Eric D.

    2009-01-01

    Objectives To estimate the prevalence of prescription medication diversion among college students; to compare classes of medications with respect to the likelihood of diversion; to document the most common methods of diversion; and to examine the characteristics of students who diverted medications. Method A cross-sectional analysis of personal interview data collected between August 2006 and August 2007 as part of an ongoing longitudinal study. The cohort of students, who were between the ages 17 and 19 at study onset, attended a large public university in the mid-Atlantic region. Information was gathered regarding a wide variety of variables, including demographics, diversion of medically prescribed drugs, illicit drug use, and childhood conduct problems. Results Among 483 students prescribed a medication, 35.8% diverted a medication at least once in their lifetime. The most commonly diverted medication classes were prescription ADHD medication, with a 61.7% diversion rate, and prescription analgesics (35.1% diversion rate). Sharing was the most common method of diversion, with 33.6% of students sharing their medication(s) and 9.3% selling in their lifetime. Comparative analyses revealed that prescription medication diverters had used more illicit drugs in the past year and had more childhood conduct problems than non-diverters. Conclusions If confirmed, these findings have important clinical implications for improved physician-patient communication and vigilance regarding prescribing analgesic and stimulant medications for young adults. PMID:20331930

  20. Genetic testing and private insurance--a case of "selling one's body"?

    PubMed

    Hübner, D

    2006-01-01

    Arguments against the possible use of genetic test results in private health and life insurance predominantly refer to the problem of certain gene carriers failing to obtain affordable insurance cover. However, some moral intuitions speaking against this practice seem to be more fundamental than mere concerns about adverse distributional effects. In their perspective, the central ethical problem is not that some people might fail to get insurance cover because of their 'bad genes', but rather that some people would manage to get insurance cover because of their 'good genes'. This paper tries to highlight the ethical background of these intuitions. Their guiding idea appears to be that, by pointing to his favourable test results, a customer might make an attempt to 'sell his body'. The rationale of this concept is developed and its applicability to the case at issue is critically investigated. The aim is to clarify an essential objection against the use of genetic information in private insurance which has not yet been openly addressed in the academic debate of the topic.

  1. Selling medical travel to US patient-consumers: the cultural appeal of website marketing messages.

    PubMed

    Sobo, Elisa J; Herlihy, Elizabeth; Bicker, Mary

    2011-04-01

    More US-based patients than ever are travelling abroad for medical or dental services. Beyond financial incentives, what cultural factors have supported this trend? Because of their interest in selling medical travel, medical travel agencies (MTAs) have vested interests in this question. To find out how they are answering it, an ethnographic content analysis of MTA websites was undertaken. Beyond themes promoting a 'worry-free experience' of 'legitimate services', themes linking healthcare consumerism to culturally specific identity ideals and self-creation/representation processes predominated. Themes relating to the demonstration of social position, savvy expression of good consumer judgment, and achievement of libertarian ideals figured highly. However, various inconsistencies (including an appeal to tourism in some but not other situations) suggested that medical travel involves, for the US-based consumer, a complex act of juggling context-specific self-identity desires and expectations in relation to healthcare. The potential impact of prevailing discourses on 'self-construction-in-practice' was explored. Findings enhance understanding of the care seeking process as experienced within the context of globalized, mass-mediated healthcare consumerism. They also point to the need for finer-grained distinctions than the global gloss 'medical travel' offers.

  2. When sex doesn't sell: using sexualized images of women reduces support for ethical campaigns.

    PubMed

    Bongiorno, Renata; Bain, Paul G; Haslam, Nick

    2013-01-01

    Images of scantily clad women are used by advertisers to make products more attractive to men. This "sex sells" approach is increasingly employed to promote ethical causes, most prominently by the animal-rights organization PETA. Yet sexualized images can dehumanize women, leaving an unresolved paradox--is it effective to advertise an ethical cause using unethical means? In Study 1, a sample of Australian male undergraduates (N = 82) viewed PETA advertisements containing either sexualized or non-sexualized images of women. Intentions to support the ethical organization were reduced for those exposed to the sexualized advertising, and this was explained by their dehumanization of the sexualized women, and not by increased arousal. Study 2 used a mixed-gender community sample from the United States (N = 280), replicating this finding and extending it by showing that behaviors helpful to the ethical cause diminished after viewing the sexualized advertisements, which was again mediated by the dehumanization of the women depicted. Alternative explanations relating to the reduced credibility of the sexualized women and their objectification were not supported. When promoting ethical causes, organizations may benefit from using advertising strategies that do not dehumanize women.

  3. Selling conscience short: a response to Schuklenk and Smalling on conscientious objections by medical professionals.

    PubMed

    Maclure, Jocelyn; Dumont, Isabelle

    2017-04-01

    In a thought-provoking paper, Schuklenk and Smalling argue that no right to conscientious objection should be granted to medical professionals. First, they hold that it is impossible to assess either the truth of conscience-based claims or the sincerity of the objectors. Second, even a fettered right to conscientious refusal inevitably has adverse effects on the rights of patients. We argue that the main problem with their position is that it is not derived from a broader reflection on the meaning and implications of freedom of conscience and reasonable accommodation. We point out that they collapse two related but distinct questions, that is, the subjective conception of freedom of conscience and the sincerity test. We note that they do not successfully show that the standard norm according to which exemption claims should not impose undue hardship on others is unworkable. We suggest that the main reason why arguments such as no one is forced to be a medical professional are flawed is that public norms should not constrain citizens to choose between two of their basic rights unless it is necessary. In fine, Schuklenk and Smalling, who see conscience claims as arbitrary dislikes, sell freedom of conscience short and forego any attempts at balancing the competing rights involved. We maintain the authors neglect that most of legal reasoning is contextual and that the blanket restriction of healthcare professionals' freedom of conscience is disproportionate.

  4. Buying on margin, selling short in an agent-based market model

    NASA Astrophysics Data System (ADS)

    Zhang, Ting; Li, Honggang

    2013-09-01

    Credit trading, or leverage trading, which includes buying on margin and selling short, plays an important role in financial markets, where agents tend to increase their leverages for increased profits. This paper presents an agent-based asset market model to study the effect of the permissive leverage level on traders’ wealth and overall market indicators. In this model, heterogeneous agents can assume fundamental value-converging expectations or trend-persistence expectations, and their effective demands of assets depend both on demand willingness and wealth constraints, where leverage can relieve the wealth constraints to some extent. The asset market price is determined by a market maker, who watches the market excess demand, and is influenced by noise factors. By simulations, we examine market results for different leverage ratios. At the individual level, we focus on how the leverage ratio influences agents’ wealth accumulation. At the market level, we focus on how the leverage ratio influences changes in the asset price, volatility, and trading volume. Qualitatively, our model provides some meaningful results supported by empirical facts. More importantly, we find a continuous phase transition as we increase the leverage threshold, which may provide a further prospective of credit trading.

  5. Sexuality sells: a content analysis of lesbian and heterosexual women's bodies in magazine advertisements.

    PubMed

    Milillo, Diana

    2008-01-01

    Controversies in the literature suggest varied views as to whether lesbian and heterosexual women accept different cultural norms about body shape and size. This article explores whether messages about the body from lesbian media deviate from mainstream, heterosexually focused media. In particular, I differentiate the messages within both media by the physical appearance of the model in the photo, and how the body is positioned in the type and context of the ad itself. Three hundred randomly selected photo advertisements from lesbian and mainstream women's magazines were examined. Lesbian models varied more in age and weight than mainstream models, and were more likely to be androgynous in gender appearance. In turn, mainstream models were more likely to be wearing revealing clothing that hindered their mobility, such as a tight skirt or stiletto heels. Advertisements in lesbian magazines placed their models in more defined contexts, such as in travel ads, and with more of a purpose than mainstream advertisements, where models were more likely to be placed in front of a non-descript backdrop. Lesbian advertisements were more likely to sell products that engendered community (e.g., travel, book club), whereas mainstream advertisements more often sold products that emphasized the self (e.g., clothing, beauty products). I consider how lesbian and heterosexual bodies are reproduced and impart messages about "doing" gender.

  6. Medico-legal aspects of congenital heart diseases in buying and selling of pets

    PubMed Central

    Passantino, Annamaria; Pugliese, Michela; Quartarone, Valeria; Russo, Natalia; Bussadori, Roberto; Guercio, Bartolomeo

    2017-01-01

    Aim: The veterinarian should be able to assess congenital and inherited malformations such as heart defects because they may be object of legal disputes. In this study, the authors report some cases of congenital heart defects in pets (dogs and cats) to clarify whether or not they may be considered a redhibitory defect. Materials and Methods: A total of 28 medical records of pets referred with suspected congenital heart disease were examined. All patients aged between 3 and 24 months underwent clinical examination, chest X-ray examination, electrocardiogram, and echocardiography and angiocardiography when necessary. Results: Congenital heart diseases or associated cardiac malformations were confirmed. Considering the above congenital diseases as redhibitory defect and the rights of the owners from a strictly legal viewpoint, 9 owners demanded an estimatory action and 11 a redhibitory action; 1 owner decided to demand the reimbursement of veterinary expenses because the animal died; 7 owners took no legal action but requested surgical intervention. Conclusions: Until more appropriate and detailed legislation on the buying and selling of pet animals is put in place; the authors propose to include in the contract a temporal extension of the guarantee relating to congenital heart disease, which can often become evident later. PMID:28246457

  7. The impact of the principal in the implementation of promoting science among English language learners (P-SELL)

    NASA Astrophysics Data System (ADS)

    Kittrell, Resma

    School organizations are in a constant state of change. One of the major changes that all schools encounter is adopting new curriculum. It is important to look at the role of the principal during the implementation of a new curriculum so that we can identify specific strategies that might be useful in other areas of school change. This study focuses on the role of the principal during the adoption of a new science curriculum, Promoting Science Among English Language Learners (P-SELL), within six elementary schools. This multiple case study included teacher focus group interviews, principal interviews, and teacher and principal written surveys to identify specific roles and strategies that principals illustrated during implementation of P-SELL. The overarching themes uncovered included (a) distributive leadership, (b) clear communication, and (c) supportive conditions. These findings can be used to understand the process of change within an organization.

  8. The University of Jaén Astronomical Observatory

    NASA Astrophysics Data System (ADS)

    Martí, Josep; Luque-Escamilla, Pedro L.; García-Hernández, María T.

    2017-01-01

    We present a description and instrumental characterization of the photometric equipment of the Astronomical Observatory of the University of Jaén. The observatory hosts a 41 cm automated telescope inside a 4 m dome located at the university main campus, in the outskirts of the city of Jaén (Spain). This facility is used for educational, outreach and occasional scientific research on bright stellar objects. Despite the observatory location in a light polluted urban area, its performance for differential photometry studies has proven to be very acceptable. The discovery of the Be star LS I +5979 as a peculiar eclipsing binary system is so far the most relevant achievement.

  9. Male Sex Workers Who Sell Sex to Men Also Engage in Anal Intercourse with Women: Evidence from Mombasa, Kenya

    PubMed Central

    Mannava, Priya; Geibel, Scott; King’ola, Nzioki; Temmerman, Marleen; Luchters, Stanley

    2013-01-01

    Objective To investigate self-report of heterosexual anal intercourse among male sex workers who sell sex to men, and to identify the socio-demographic characteristics associated with practice of the behavior. Design Two cross-sectional surveys of male sex workers who sell sex to men in Mombasa, Kenya. Methods Male sex workers selling sex to men were invited to participate in surveys undertaken in 2006 and 2008. A structured questionnaire administered by trained interviewers was used to collect information on socio-demographic characteristics, sexual behaviors, HIV and STI knowledge, and health service usage. Data were analyzed through descriptive and inferential statistics. Bivariate logistic regression, after controlling for year of survey, was used to identify socio-demographic characteristics associated with heterosexual anal intercourse. Results From a sample of 867 male sex workers, 297 men had sex with a woman during the previous 30 days – of whom 45% did so with a female client and 86% with a non-paying female partner. Within these groups, 66% and 43% of male sex workers had anal intercourse with a female client and non-paying partner respectively. Factors associated with reporting recent heterosexual anal intercourse in bivariate logistic regression after controlling for year of survey participation were being Muslim, ever or currently married, living with wife only, living with a female partner only, living with more than one sexual partner, self-identifying as basha/king/bisexual, having one’s own children, and lower education. Conclusions We found unexpectedly high levels of self-reported anal sex with women by male sex workers, including selling sex to female clients as well as with their own partners. Further investigation among women in Mombasa is needed to understand heterosexual anal sex practices, and how HIV programming may respond. PMID:23300978

  10. Underage and underserved: reaching young women who sell sex in Zimbabwe

    PubMed Central

    Busza, Joanna; Mtetwa, Sibongile; Mapfumo, Rumbidzo; Hanisch, Dagmar; Wong-Gruenwald, Ramona; Cowan, Frances

    2016-01-01

    ABSTRACT Young women who sell sex (YWSS) in Southern Africa are highly vulnerable to HIV, as the risks of being young and female in a high prevalence setting coalesce with those of commercial sex. YWSS are less able to negotiate safe sex, more likely to have higher risk partners, and less likely to use available health services compared to older sex workers. In Zimbabwe’s national HIV programme for sex workers, fewer than 1% of clients were 15–29. We developed monthly interactive workshops for YWSS based on an Activity Pack consisting of 21 sessions organised into six modules. The aim was to encourage YWSS’ interaction with each other, build their trust, confidence and skills, and encourage uptake of clinical services. We conducted a process evaluation to assess programme strengths, identify challenges, and recommend changes. This paper presents findings synthesising programme records with qualitative data and discusses feasibility, acceptability, and outputs during the pilot phase. In total, 143 YWSS attended meetings and most were from the target 15–19-year-old age group. Participants enjoyed the sessions and reported improved cooperation, willingness to negotiate with clients, and self-reflection about their futures. Staff found facilitating sessions easy and activities clear and appropriate. Challenges included identifying appropriate referrals, initial recruitment of women in some sites, and managing participants’ requests for financial compensation. The number of clients aged 15–19 increased at sex worker clinics in all sites. This programme is the first to target YWSS in Zimbabwe to address their disproportionately low service use. It proved feasible to staff and acceptable to participants over a one-year period. Given enhanced vulnerability of YWSS, this programme provides one workable model for reaching this underserved group. PMID:27391994

  11. Quality of Online Pharmacies and Websites Selling Prescription Drugs: A Systematic Review

    PubMed Central

    Merla, Anna; Schulz, Peter J; Gelatti, Umberto

    2011-01-01

    Background Online pharmacies are companies that sell pharmaceutical preparations, including prescription-only drugs, on the Internet. Very little is known about this phenomenon because many online pharmacies operate from remote countries, where legal bases and business practices are largely inaccessible to international research. Objective The aim of the study was to perform an up-to-date and comprehensive review of the scientific literature focusing on the broader picture of online pharmacies by scanning several scientific and institutional databases, with no publication time limits. Methods We searched 4 electronic databases up to January 2011 and the gray literature on the Internet using the Google search engine and its tool Google Scholar. We also investigated the official websites of institutional agencies (World Health Organization, and US and European centers for disease control and drug regulation authorities). We focused specifically on online pharmacies offering prescription-only drugs. We decided to analyze and report only articles with original data, in order to review all the available data regarding online pharmacies and their usage. Results We selected 193 relevant articles: 76 articles with original data, and 117 articles without original data (editorials, regulation articles, or the like) including 5 reviews. The articles with original data cover samples of online pharmacies in 47 cases, online drug purchases in 13, consumer characteristics in 15, and case reports on adverse effects of online drugs in 12. The studies show that random samples with no specific limits to prescription requirements found that at least some websites sold drugs without a prescription and that an online questionnaire was a frequent tool to replace prescription. Data about geographical characteristics show that this information can be concealed in many websites. The analysis of drug offer showed that online a consumer can get virtually everything. Regarding quality of drugs

  12. Increased SA in NPR1-silenced plants antagonizes JA and JA-dependent direct and indirect defenses in herbivore-attacked Nicotiana attenuata in nature.

    PubMed

    Rayapuram, Cbgowda; Baldwin, Ian T

    2007-11-01

    The phytohormone jasmonic acid (JA) is known to mediate herbivore resistance, while salicylic acid (SA) and non-expressor of PR-1 (NPR1) mediate pathogen resistance in many plants. Herbivore attack on Nicotiana attenuata elicits increases in JA and JA-mediated defenses, but also increases SA levels and Na-NPR1 transcripts from the plant's single genomic copy. SA treatment of wild-type plants increases Na-NPR1 and Na-PR1 transcripts. Plants silenced in NPR1 accumulation by RNAi (ir-npr1) are highly susceptible to herbivore and pathogen attack when planted in their native habitat in Utah. They are also impaired in their ability to attract Geocorus pallens predators, due to their decreased ability to release cis-alpha-bergamotene, a JA-elicited volatile 'alarm call'. In the glasshouse, Spodoptera exigua larvae grew better on ir-npr1 plants, which had low levels of JA, JA-isoleucine/leucine, lipoxygenase-3 (LOX3) transcripts and JA-elicited direct defense metabolites (nicotine, caffeoyl putrescine and rutin), but high levels of SA and isochorismate synthase (ICS) transcripts, suggesting de novo biosynthesis of SA. A microarray analysis revealed downregulation of many JA-elicited genes and upregulation of SA biosynthetic genes. JA treatment restored nicotine levels and resistance to S. exigua in ir-npr1 plants. We conclude that, during herbivore attack, NPR1 negatively regulates SA production, allowing the unfettered elicitation of JA-mediated defenses; when NPR1 is silenced, the elicited increases in SA production antagonize JA and JA-related defenses, making the plants susceptible to herbivores.

  13. Supply chain coordination with two production modes and random demand depending on advertising expenditure and selling price

    NASA Astrophysics Data System (ADS)

    Wang, Sheng-Dong; Zhou, Yong-Wu; Wang, Jun-Ping

    2010-10-01

    This article discusses production and order as well as advertising coordination issues in a single-manufacturer single-buyer supply chain, where the manufacturer sells a newsvendor-type product through the buyer who faces a random demand depending on advertising expenditure and selling price. The buyer has two ordering opportunities: the one happens before the beginning of the season, and the other takes place at the end of the season. The ordered items are produced by the manufacturer in two production modes for different requirements. The first production mode is relatively cheap but requires a long lead-time, whereas the second is expensive but offers quick response. Under such a setting, the centralised and decentralised decision models are developed, respectively, and the closed form solution to each model is provided as well. Moreover, we point out that the traditional revenue-sharing contract fails to coordinate the supply chain. We thus propose an improved revenue-sharing contract that requests the manufacturer not only shares the buyer's revenue but also bears a portion of the buyer's operating costs. Such a contract can achieve perfect coordination of the supply chain and arbitrarily allocate its profit between two parties.

  14. HIV risk among drug-using men who have sex with men, men selling sex, and transgender individuals in Vietnam.

    PubMed

    Vu, Bao Ngoc; Mulvey, Kevin P; Baldwin, Simon; Nguyen, Son Thanh

    2012-01-01

    Knowledge about drug use and its association with HIV risk among men who have sex with men is limited. Although the HIV epidemic among this population in Vietnam is increasingly acknowledged, understanding the impact of drug use on the spread of HIV is largely lacking. Using qualitative data from in-depth interviews and focus group discussions with 93 drug users, 15 non-drug users and 9 community stakeholders, this analysis explores emerging patterns of drug use and risk factors for engaging in risk behaviours among drug-using men having sex with men, men selling sex and transgender individuals in Hanoi and Ho Chi Minh City. Findings revealed that drug use is shifting from heroin to ecstasy and ice. Drug users reported unsafe sex associated with drug use and men selling sex were particularly at elevated risk because of using drugs as a tool for sex work and trading sex for drugs. These findings are guiding development of programmes addressing unmet HIV-prevention needs in Vietnam.

  15. Coordinate expression of AOS genes and JA accumulation: JA is not required for initiation of closing layer in wound healing tubers

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Wounding induces a series of coordinated physiological responses essential for protection and healing of the damaged tissue. Wound-induced formation of jasmonic acid (JA) is important in defense responses in leaves, but comparatively little is known about the induction of JA biosynthesis and its ro...

  16. Forcing the Navy to sell cigarettes on ships: how the tobacco industry and politicians torpedoed Navy tobacco control.

    PubMed

    Offen, Naphtali; Arvey, Sarah R; Smith, Elizabeth A; Malone, Ruth E

    2011-03-01

    In 1986, the US Navy announced the goal of becoming smoke-free by 2000. However, efforts to restrict tobacco sales and use aboard the USS Roosevelt prompted tobacco industry lobbyists to persuade their allies in Congress to legislate that all naval ships must sell tobacco. Congress also removed control of ships' stores from the Navy. By 1993, the Navy abandoned its smoke-free goal entirely and promised smokers a place to smoke on all ships. Congressional complicity in promoting the agenda of the tobacco industry thwarted the Navy's efforts to achieve a healthy military workforce. Because of military lobbying constraints, civilian pressure on Congress may be necessary to establish effective tobacco control policies in the armed forces.

  17. Forcing the Navy to Sell Cigarettes on Ships: How the Tobacco Industry and Politicians Torpedoed Navy Tobacco Control

    PubMed Central

    Arvey, Sarah R.; Smith, Elizabeth A.; Malone, Ruth E.

    2011-01-01

    In 1986, the US Navy announced the goal of becoming smoke-free by 2000. However, efforts to restrict tobacco sales and use aboard the USS Roosevelt prompted tobacco industry lobbyists to persuade their allies in Congress to legislate that all naval ships must sell tobacco. Congress also removed control of ships’ stores from the Navy. By 1993, the Navy abandoned its smoke-free goal entirely and promised smokers a place to smoke on all ships. Congressional complicity in promoting the agenda of the tobacco industry thwarted the Navy's efforts to achieve a healthy military workforce. Because of military lobbying constraints, civilian pressure on Congress may be necessary to establish effective tobacco control policies in the armed forces. PMID:21233435

  18. The mealybug Phenacoccus solenopsis suppresses plant defense responses by manipulating JA-SA crosstalk

    PubMed Central

    Zhang, Peng-Jun; Huang, Fang; Zhang, Jin-Ming; Wei, Jia-Ning; Lu, Yao-Bin

    2015-01-01

    Induced plant defenses against herbivores are modulated by jasmonic acid-, salicylic acid-, and ethylene-signaling pathways. Although there is evidence that some pathogens suppress plant defenses by interfering with the crosstalk between different signaling pathways, such evidence is scarce for herbivores. Here, we demonstrate that the mealybug Phenacoccus solenopsis suppresses the induced defenses in tomato. We found that exogenous JA, but not SA, significantly decreased mealybug feeding time and reduced nymphal performance. In addition, constitutive activation of JA signaling in 35s::prosys plants reduced mealybug survival. These data indicate that the JA signaling pathway plays a key role in mediating the defense responses against P. solenopsis. We also found that mealybug feeding decreased JA production and JA-dependent defense gene expression, but increased SA accumulation and SA-dependent gene expression. In SA-deficient plants, mealybug feeding did not suppress but activated JA accumulation, indicating that the suppression of JA-regulated defenses depends on the SA signaling pathway. Mealybugs benefit from suppression of JA-regulated defenses by exhibiting enhanced nymphal performance. These findings confirm that P. solenopsis manipulates plants for its own benefits by modulating the JA-SA crosstalk and thereby suppressing induced defenses. PMID:25790868

  19. Synthesis, structural characterization and biological activity of two diastereomeric JA-Ile macrolactones.

    PubMed

    Jimenez-Aleman, Guillermo H; Machado, Ricardo A R; Görls, Helmar; Baldwin, Ian T; Boland, Wilhelm

    2015-06-07

    Jasmonates are phytohormones involved in a wide range of plant processes, including growth, development, senescence, and defense. Jasmonoyl-L-isoleucine (JA-Ile, 2), an amino acid conjugate of jasmonic acid (JA, 1), has been identified as a bioactive endogenous jasmonate. However, JA-Ile (2) analogues trigger different responses in the plant. ω-Hydroxylation of the pentenyl side chain leads to the inactive 12-OH-JA-Ile (3) acting as a “stop” signal. On the other hand, a lactone derivative of 12-OH-JA (5) (jasmine ketolactone, JKL) occurs in nature, although with no known biological function. Inspired by the chemical structure of JKL (6) and in order to further explore the potential biological activities of 12-modified JA-Ile derivatives, we synthesized two macrolactones (JA-Ile-lactones (4a) and (4b)) derived from 12-OH-JA-Ile (3). The biological activity of (4a) and (4b) was tested for their ability to elicit nicotine production, a well-known jasmonate dependent secondary metabolite. Both macrolactones showed strong biological activity, inducing nicotine accumulation to a similar extent as methyl jasmonate does in Nicotiana attenuata leaves. Surprisingly, the highest nicotine contents were found in plants treated with the JA-Ile-lactone (4b), which has (3S,7S) configuration at the cyclopentanone not known from natural jasmonates. Macrolactone (4a) is a valuable standard to explore for its occurrence in nature.

  20. Selling. Unit 16. Level 1. Instructor Guide. PACE: Program for Acquiring Competence in Entrepreneurship. Third Edition. Research & Development Series No. 301-16.

    ERIC Educational Resources Information Center

    Ohio State Univ., Columbus. Center on Education and Training for Employment.

    This instructor guide for a unit on selling in the PACE (Program for Acquiring Competence in Entrepreneurship) curriculum includes the full text of the student module and lesson plans, instructional suggestions, and other teacher resources. The competencies that are incorporated into this module are at Level 1 of learning--understanding the…

  1. 12 CFR 550.350 - May I lend, sell, or transfer assets of a fiduciary account if I have an interest in the...

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... which you have investment discretion to yourself or any of your directors, officers, or employees; to...) Exceptions—(1) Funds for which you have investment discretion. You may lend, sell or otherwise transfer assets of a fiduciary account for which you have investment discretion to yourself or any of...

  2. 30 CFR 206.53 - How do I determine value for oil that I or my affiliate do(es) not sell under an arm's-length...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 30 Mineral Resources 2 2010-07-01 2010-07-01 false How do I determine value for oil that I or my affiliate do(es) not sell under an arm's-length contract? 206.53 Section 206.53 Mineral Resources MINERALS MANAGEMENT SERVICE, DEPARTMENT OF THE INTERIOR MINERALS REVENUE MANAGEMENT PRODUCT VALUATION Indian Oil §...

  3. 41 CFR 302-12.7 - What expenses will my agency pay if I use a relocation services company to sell or purchase a...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 41 Public Contracts and Property Management 4 2011-07-01 2011-07-01 false What expenses will my agency pay if I use a relocation services company to sell or purchase a residence for which I and/or a... Property Management Federal Travel Regulation System RELOCATION ALLOWANCES RESIDENCE TRANSACTION...

  4. 41 CFR 302-12.7 - What expenses will my agency pay if I use a relocation services company to sell or purchase a...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 41 Public Contracts and Property Management 4 2010-07-01 2010-07-01 false What expenses will my agency pay if I use a relocation services company to sell or purchase a residence for which I and/or a... Property Management Federal Travel Regulation System RELOCATION ALLOWANCES RESIDENCE TRANSACTION...

  5. 12 CFR 225.136 - Utilization of foreign subsidiaries to sell long-term debt obligations in foreign markets and to...

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 12 Banks and Banking 3 2011-01-01 2011-01-01 false Utilization of foreign subsidiaries to sell long-term debt obligations in foreign markets and to transfer the proceeds to their United States... CONTROL (REGULATION Y) Regulations Financial Holding Companies Interpretations § 225.136 Utilization...

  6. 30 CFR 1206.355 - How do I calculate royalty due on geothermal resources I sell at arm's length to a purchaser for...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 30 Mineral Resources 3 2013-07-01 2013-07-01 false How do I calculate royalty due on geothermal resources I sell at arm's length to a purchaser for direct use? 1206.355 Section 1206.355 Mineral Resources OFFICE OF NATURAL RESOURCES REVENUE, DEPARTMENT OF THE INTERIOR NATURAL RESOURCES REVENUE...

  7. 30 CFR 1206.355 - How do I calculate royalty due on geothermal resources I sell at arm's length to a purchaser for...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 30 Mineral Resources 3 2012-07-01 2012-07-01 false How do I calculate royalty due on geothermal resources I sell at arm's length to a purchaser for direct use? 1206.355 Section 1206.355 Mineral Resources OFFICE OF NATURAL RESOURCES REVENUE, DEPARTMENT OF THE INTERIOR NATURAL RESOURCES REVENUE...

  8. 30 CFR 1206.53 - How do I determine value for oil that I or my affiliate do(es) not sell under an arm's-length...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 30 Mineral Resources 3 2013-07-01 2013-07-01 false How do I determine value for oil that I or my affiliate do(es) not sell under an arm's-length contract? 1206.53 Section 1206.53 Mineral Resources OFFICE OF NATURAL RESOURCES REVENUE, DEPARTMENT OF THE INTERIOR NATURAL RESOURCES REVENUE PRODUCT...

  9. 30 CFR 1206.355 - How do I calculate royalty due on geothermal resources I sell at arm's length to a purchaser for...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 30 Mineral Resources 3 2014-07-01 2014-07-01 false How do I calculate royalty due on geothermal resources I sell at arm's length to a purchaser for direct use? 1206.355 Section 1206.355 Mineral Resources OFFICE OF NATURAL RESOURCES REVENUE, DEPARTMENT OF THE INTERIOR NATURAL RESOURCES REVENUE...

  10. 17 CFR 270.10f-3 - Exemption for the acquisition of securities during the existence of an underwriting or selling...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 17 Commodity and Securities Exchanges 3 2011-04-01 2011-04-01 false Exemption for the acquisition of securities during the existence of an underwriting or selling syndicate. 270.10f-3 Section 270.10f-3 Commodity and Securities Exchanges SECURITIES AND EXCHANGE COMMISSION (CONTINUED) RULES...

  11. 17 CFR 270.10f-3 - Exemption for the acquisition of securities during the existence of an underwriting or selling...

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 17 Commodity and Securities Exchanges 3 2012-04-01 2012-04-01 false Exemption for the acquisition of securities during the existence of an underwriting or selling syndicate. 270.10f-3 Section 270.10f-3 Commodity and Securities Exchanges SECURITIES AND EXCHANGE COMMISSION (CONTINUED) RULES...

  12. 17 CFR 270.10f-3 - Exemption for the acquisition of securities during the existence of an underwriting or selling...

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... of securities during the existence of an underwriting or selling syndicate. 270.10f-3 Section 270.10f-3 Commodity and Securities Exchanges SECURITIES AND EXCHANGE COMMISSION (CONTINUED) RULES AND REGULATIONS, INVESTMENT COMPANY ACT OF 1940 § 270.10f-3 Exemption for the acquisition of securities during...

  13. 17 CFR 270.10f-3 - Exemption for the acquisition of securities during the existence of an underwriting or selling...

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 17 Commodity and Securities Exchanges 3 2013-04-01 2013-04-01 false Exemption for the acquisition of securities during the existence of an underwriting or selling syndicate. 270.10f-3 Section 270.10f-3 Commodity and Securities Exchanges SECURITIES AND EXCHANGE COMMISSION (CONTINUED) RULES...

  14. 25 CFR 309.7 - How should a seller disclose the nature and degree of Indian labor when selling, offering, or...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... Indian labor when selling, offering, or displaying art and craft work for sale? 309.7 Section 309.7 Indians INDIAN ARTS AND CRAFTS BOARD, DEPARTMENT OF THE INTERIOR PROTECTION OF INDIAN ARTS AND CRAFTS..., or displaying art and craft work for sale? The Indian Arts and Crafts Act is a truth-in-marketing...

  15. 25 CFR 309.7 - How should a seller disclose the nature and degree of Indian labor when selling, offering, or...

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... Indian labor when selling, offering, or displaying art and craft work for sale? 309.7 Section 309.7 Indians INDIAN ARTS AND CRAFTS BOARD, DEPARTMENT OF THE INTERIOR PROTECTION OF INDIAN ARTS AND CRAFTS..., or displaying art and craft work for sale? The Indian Arts and Crafts Act is a truth-in-marketing...

  16. 25 CFR 309.7 - How should a seller disclose the nature and degree of Indian labor when selling, offering, or...

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... Indian labor when selling, offering, or displaying art and craft work for sale? 309.7 Section 309.7 Indians INDIAN ARTS AND CRAFTS BOARD, DEPARTMENT OF THE INTERIOR PROTECTION OF INDIAN ARTS AND CRAFTS..., or displaying art and craft work for sale? The Indian Arts and Crafts Act is a truth-in-marketing...

  17. 25 CFR 309.7 - How should a seller disclose the nature and degree of Indian labor when selling, offering, or...

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... Indian labor when selling, offering, or displaying art and craft work for sale? 309.7 Section 309.7 Indians INDIAN ARTS AND CRAFTS BOARD, DEPARTMENT OF THE INTERIOR PROTECTION OF INDIAN ARTS AND CRAFTS..., or displaying art and craft work for sale? The Indian Arts and Crafts Act is a truth-in-marketing...

  18. 25 CFR 309.7 - How should a seller disclose the nature and degree of Indian labor when selling, offering, or...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... Indian labor when selling, offering, or displaying art and craft work for sale? 309.7 Section 309.7 Indians INDIAN ARTS AND CRAFTS BOARD, DEPARTMENT OF THE INTERIOR PROTECTION OF INDIAN ARTS AND CRAFTS..., or displaying art and craft work for sale? The Indian Arts and Crafts Act is a truth-in-marketing...

  19. 40 CFR 1068.260 - What general provisions apply for selling or shipping engines that are not yet in their certified...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... section specifies clarifications and exemptions related to these requirements for engines. Except for... parameters. (1) You do not need an exemption to ship an engine that does not include installation or assembly... selling or shipping engines that are not yet in their certified configuration? 1068.260 Section...

  20. 40 CFR 1068.260 - What general provisions apply for selling or shipping engines that are not yet in their certified...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... clarifications and exemptions related to these requirements for engines. Except for paragraph (c) of this section... need an exemption to ship an engine that does not include installation or assembly of certain emission... selling or shipping engines that are not yet in their certified configuration? 1068.260 Section...

  1. 40 CFR 1068.260 - What general provisions apply for selling or shipping engines that are not yet in their certified...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... clarifications and exemptions related to these requirements for engines. Except for paragraph (c) of this section... need an exemption to ship an engine that does not include installation or assembly of certain emission... selling or shipping engines that are not yet in their certified configuration? 1068.260 Section...

  2. Techno-Economic Analysis of the Deacetylation and Disk Refining Process. Characterizing the Effect of Refining Energy and Enzyme Usage on Minimum Sugar Selling Price and Minimum Ethanol Selling Price

    SciTech Connect

    Chen, Xiaowen; Shekiro, Joseph; Pschorn, Thomas; Sabourin, Marc; Tucker, Melvin P.; Tao, Ling

    2015-10-29

    A novel, highly efficient deacetylation and disk refining (DDR) process to liberate fermentable sugars from biomass was recently developed at the National Renewable Energy Laboratory (NREL). The DDR process consists of a mild, dilute alkaline deacetylation step followed by low-energy-consumption disk refining. The DDR corn stover substrates achieved high process sugar conversion yields, at low to modest enzyme loadings, and also produced high sugar concentration syrups at high initial insoluble solid loadings. The sugar syrups derived from corn stover are highly fermentable due to low concentrations of fermentation inhibitors. The objective of this work is to evaluate the economic feasibility of the DDR process through a techno-economic analysis (TEA). A large array of experiments designed using a response surface methodology was carried out to investigate the two major cost-driven operational parameters of the novel DDR process: refining energy and enzyme loadings. The boundary conditions for refining energy (128–468 kWh/ODMT), cellulase (Novozyme’s CTec3) loading (11.6–28.4 mg total protein/g of cellulose), and hemicellulase (Novozyme’s HTec3) loading (0–5 mg total protein/g of cellulose) were chosen to cover the most commercially practical operating conditions. The sugar and ethanol yields were modeled with good adequacy, showing a positive linear correlation between those yields and refining energy and enzyme loadings. The ethanol yields ranged from 77 to 89 gallons/ODMT of corn stover. The minimum sugar selling price (MSSP) ranged from $0.191 to $0.212 per lb of 50 % concentrated monomeric sugars, while the minimum ethanol selling price (MESP) ranged from $2.24 to $2.54 per gallon of ethanol. The DDR process concept is evaluated for economic feasibility through TEA. The MSSP and MESP of the DDR process falls within a range similar to that found with the deacetylation/dilute acid pretreatment process modeled in NREL’s 2011 design report. The DDR

  3. Techno-Economic Analysis of the Deacetylation and Disk Refining Process. Characterizing the Effect of Refining Energy and Enzyme Usage on Minimum Sugar Selling Price and Minimum Ethanol Selling Price

    DOE PAGES

    Chen, Xiaowen; Shekiro, Joseph; Pschorn, Thomas; ...

    2015-10-29

    A novel, highly efficient deacetylation and disk refining (DDR) process to liberate fermentable sugars from biomass was recently developed at the National Renewable Energy Laboratory (NREL). The DDR process consists of a mild, dilute alkaline deacetylation step followed by low-energy-consumption disk refining. The DDR corn stover substrates achieved high process sugar conversion yields, at low to modest enzyme loadings, and also produced high sugar concentration syrups at high initial insoluble solid loadings. The sugar syrups derived from corn stover are highly fermentable due to low concentrations of fermentation inhibitors. The objective of this work is to evaluate the economic feasibilitymore » of the DDR process through a techno-economic analysis (TEA). A large array of experiments designed using a response surface methodology was carried out to investigate the two major cost-driven operational parameters of the novel DDR process: refining energy and enzyme loadings. The boundary conditions for refining energy (128–468 kWh/ODMT), cellulase (Novozyme’s CTec3) loading (11.6–28.4 mg total protein/g of cellulose), and hemicellulase (Novozyme’s HTec3) loading (0–5 mg total protein/g of cellulose) were chosen to cover the most commercially practical operating conditions. The sugar and ethanol yields were modeled with good adequacy, showing a positive linear correlation between those yields and refining energy and enzyme loadings. The ethanol yields ranged from 77 to 89 gallons/ODMT of corn stover. The minimum sugar selling price (MSSP) ranged from $0.191 to $0.212 per lb of 50 % concentrated monomeric sugars, while the minimum ethanol selling price (MESP) ranged from $2.24 to $2.54 per gallon of ethanol. The DDR process concept is evaluated for economic feasibility through TEA. The MSSP and MESP of the DDR process falls within a range similar to that found with the deacetylation/dilute acid pretreatment process modeled in NREL’s 2011 design report. The

  4. Novel bioactive oxazolomycin isomers produced by Streptomyces albus JA3453.

    PubMed

    Kanzaki, H; Wada, K; Nitoda, T; Kawazu, K

    1998-03-01

    Two novel oxazolomycin isomers, oxazolomycins B (2) and C (3), were isolated from the fermentation broth of an oxazolomycin-producing strain, Streptomyces albus JA3453. Both compounds are geometrical isomers of oxazolomycin (1), the configurations of their triene moieties being (4'E, 6'E, 8'E) (2) and (4'Z, 6'E, 8'E) (3) while that of oxazolomycin (1) is (4'Z, 6'Z, 8'E). Compounds 2 and 3 exhibited potent inhibitory activity against crown gall formation with the same MIC (0.8 microgram/disk) as oxazolomycin. Compounds 2 and 3 showed no antibacterial activity against Agrobacterium tumefaciens, in contrast to oxazolomycin which has specific anti-A. tumefaciens activity.

  5. First results on applying a non-linear effect formalism to alliances between political parties and buy and sell dynamics

    NASA Astrophysics Data System (ADS)

    Bagarello, F.; Haven, E.

    2016-02-01

    We discuss a non linear extension of a model of alliances in politics, recently proposed by one of us. The model is constructed in terms of operators, describing the interest of three parties to form, or not, some political alliance with the other parties. The time evolution of what we call the decision functions is deduced by introducing a suitable Hamiltonian, which describes the main effects of the interactions of the parties amongst themselves and with their environments, which are generated by their electors and by people who still have no clear idea for which party to vote (or even if to vote). The Hamiltonian contains some non-linear effects, which takes into account the role of a party in the decision process of the other two parties. Moreover, we show how the same Hamiltonian can also be used to construct a formal structure which can describe the dynamics of buying and selling financial assets (without however implying a specific price setting mechanism).

  6. THE PROGENITOR OF SN 2011ja: CLUES FROM CIRCUMSTELLAR INTERACTION

    SciTech Connect

    Chakraborti, Sayan; Ray, Alak; Yadav, Naveen; Smith, Randall; Ryder, Stuart; Sutaria, Firoza; Dwarkadas, Vikram V.; Chandra, Poonam; Pooley, David; Roy, Rupak

    2013-09-01

    Massive stars, possibly red supergiants, which retain extended hydrogen envelopes until core collapse, produce Type II plateau (IIP) supernovae. The ejecta from these explosions shocks the circumstellar matter originating from the mass loss of the progenitor during the final phases of its life. This interaction accelerates particles to relativistic energies which then lose energy via synchrotron radiation in the shock-amplified magnetic fields and inverse Compton scattering against optical photons from the supernova. These processes produce different signatures in the radio and X-ray parts of the electromagnetic spectrum. Observed together, they allow us to break the degeneracy between shock acceleration and magnetic field amplification. In this work, we use X-rays observations from the Chandra and radio observations from the Australia Telescope Compact Array to study the relative importance of processes which accelerate particles and those which amplify magnetic fields in producing the non-thermal radiation from SN 2011ja. We use radio observations to constrain the explosion date. Multiple Chandra observations allow us to probe the history of variable mass loss from the progenitor. The ejecta expands into a low-density bubble followed by interaction with a higher density wind from a red supergiant consistent with M{sub ZAMS} {approx}> 12 M{sub Sun }. Our results suggest that a fraction of Type IIP supernovae may interact with circumstellar media set up by non-steady winds.

  7. Continuous Dust Formation in SNe 2010jl and 2011ja

    NASA Astrophysics Data System (ADS)

    Krafton, Kelsie; Clayton, Geoffrey; Andrews, Jennifer; Barlow, Michael; De Looze, Ilse

    2016-08-01

    Studies in the last 10 years of dust formation in core-collapse supernovae (CCSNe) have found only small amounts, ~0.001 solar masses. This is far less than the amount needed to account for the large masses of dust seen in some high redshift galaxies. However, the recent discovery of ~1 solar mass of cold dust in the ejecta of SN 1987A has has caused a complete re-evaluation of dust formation in CCSNe. It has been suggested that the CCSNe are continuously forming dust so that by the time they are about 25 years old they will have dust masses similar to SN 1987A. However, there is a wide time gap between the CCSNe that have been studied recently and SN 1987A. We plan to use the sensitivity of Spitzer to detect dust emission from CCSNe 5 or more years after explosion. Radiative transfer models will be used to estimate the dust masses. This proposal is to continue our study of two interesting SNe 2010jl and 2011ja. These observations are part of a long term study requiring multiple epochs of Spitzer observations to look for evidence of continuous dust formation. These observations will help shed light on the mystery of dust in SN 1987A.

  8. Financial Knudsen number: Breakdown of continuous price dynamics and asymmetric buy-and-sell structures confirmed by high-precision order-book information

    NASA Astrophysics Data System (ADS)

    Yura, Yoshihiro; Takayasu, Hideki; Sornette, Didier; Takayasu, Misako

    2015-10-01

    We generalize the description of the dynamics of the order book of financial markets in terms of a Brownian particle embedded in a fluid of incoming, exiting, and annihilating particles by presenting a model of the velocity on each side (buy and sell) independently. The improved model builds on the time-averaged number of particles in the inner layer and its change per unit time, where the inner layer is revealed by the correlations between price velocity and change in the number of particles (limit orders). This allows us to introduce the Knudsen number of the financial Brownian particle motion and its asymmetric version (on the buy and sell sides). Not being considered previously, the asymmetric Knudsen numbers are crucial in finance in order to detect asymmetric price changes. The Knudsen numbers allows us to characterize the conditions for the market dynamics to be correctly described by a continuous stochastic process. Not questioned until now for large liquid markets such as the USD-JPY and EUR-USD exchange rates, we show that there are regimes when the Knudsen numbers are so high that discrete particle effects dominate, such as during market stresses and crashes. We document the presence of imbalances of particles depletion rates on the buy and sell sides that are associated with high Knudsen numbers and violent directional price changes. This indicator can detect the direction of the price motion at the early stage while the usual volatility risk measure is blind to the price direction.

  9. Financial Knudsen number: Breakdown of continuous price dynamics and asymmetric buy-and-sell structures confirmed by high-precision order-book information.

    PubMed

    Yura, Yoshihiro; Takayasu, Hideki; Sornette, Didier; Takayasu, Misako

    2015-10-01

    We generalize the description of the dynamics of the order book of financial markets in terms of a Brownian particle embedded in a fluid of incoming, exiting, and annihilating particles by presenting a model of the velocity on each side (buy and sell) independently. The improved model builds on the time-averaged number of particles in the inner layer and its change per unit time, where the inner layer is revealed by the correlations between price velocity and change in the number of particles (limit orders). This allows us to introduce the Knudsen number of the financial Brownian particle motion and its asymmetric version (on the buy and sell sides). Not being considered previously, the asymmetric Knudsen numbers are crucial in finance in order to detect asymmetric price changes. The Knudsen numbers allows us to characterize the conditions for the market dynamics to be correctly described by a continuous stochastic process. Not questioned until now for large liquid markets such as the USD-JPY and EUR-USD exchange rates, we show that there are regimes when the Knudsen numbers are so high that discrete particle effects dominate, such as during market stresses and crashes. We document the presence of imbalances of particles depletion rates on the buy and sell sides that are associated with high Knudsen numbers and violent directional price changes. This indicator can detect the direction of the price motion at the early stage while the usual volatility risk measure is blind to the price direction.

  10. Statistical properties of short-selling and margin-trading activities and their impacts on returns in the Chinese stock markets

    NASA Astrophysics Data System (ADS)

    Gao, Yan; Gao, Yao

    2015-11-01

    We investigate the collective behaviors of short-selling and margin-trading between Chinese stocks and their impacts on the co-movements of stock returns by cross-correlation and partial correlation analyses. We find that the collective behaviors of margin-trading are largely attributed to the index cohesive force, while those of short-selling are mainly due to some direct interactions between stocks. Interestingly, the dominant role the finance industry plays in the collective behaviors of short-selling could make it more important in affecting the co-movement structure of stock returns by strengthening its relationship with the market index. By detecting the volume-return and volume-volatility relationships, we find that the investors of the two leverage activities are positively triggered by individual stock volatility first, and next, at the return level, margin-buyers show trend-following properties, while short-sellers are probably informative traders who trade on the information impulse of specific firms. However, the return predictability of the two leverage trading activities and their impacts on stock volatility are not significant. Moreover, both tails of the cumulative distributions of the two leverage trading activities are found following the stretched exponential law better than the power-law.

  11. Buying and Selling “Loosies” in Baltimore: The Informal Exchange of Cigarettes in the Community Context

    PubMed Central

    Stillman, Frances; Bone, Lee; Yancey, Norman; Price, Emmanuel; Belin, Precilla; Kromm, Elizabeth Edsall

    2007-01-01

    Since the release of the first Surgeon General’s report, the proportion of adult smokers in the U.S. has been reduced by half (U.S. Department of Health and Human Services, 2004). This success has not, however, been equally felt across all social strata. Recent survey data from Baltimore show considerably elevated smoking rates within urban, African-American communities. Of particular concern was that in some communities, over half of the young adults (18–24 years old) smoke cigarettes. As yet, there has been little focus on understanding or preventing cigarette smoking among young adults, particularly for those seeking entry into the workforce rather than being engaged in higher education. In this paper, we explore community factors contributing to high young adult smoking prevalence. Our analysis is based on data from four focus groups conducted in 2004 as part of a community-based participatory research project with two urban education and job training organizations. The focus group data reflect the experiences and opinions of 28 young adult program participants (23 smokers and 5 nonsmokers). The data highlight a normalized practice of buying and selling single cigarettes (“loosies”) within the community, with participants describing buying loose cigarettes as a preferred acquisition practice. We apply theories of informal economy and suggest that this alternative purchasing option may influence the smoking behavior of these young adults. We argue that public health efforts need to more closely consider the impact of community structures on program implementation. Overlooking key community characteristics such as the availability of single cigarettes may serve to intensify health disparities. PMID:17431795

  12. JaK/STAT Inhibition to Prevent Post-Traumatic Epileptogenesis

    DTIC Science & Technology

    2014-09-01

    associated with epileptogenesis were determined. It was determined that upregulation of the JaK/STAT pathway in the injured hippocampus occurs after CCI...inhibitor, WP1066. Blocking JaK/STAT3 activity did not prevent loss of GABA cells in the injured hippocampus . Inhibitory postsynaptic currents in the...reduce development of post-traumatic epilepsy, and did not significantly improve memory function, but did enhance the motor recovery. These findings

  13. Shared binding sites in Lepidoptera for Bacillus thuringiensis Cry1Ja and Cry1A toxins.

    PubMed

    Herrero, S; González-Cabrera, J; Tabashnik, B E; Ferré, J

    2001-12-01

    Bacillus thuringiensis toxins act by binding to specific target sites in the insect midgut epithelial membrane. The best-known mechanism of resistance to B. thuringiensis toxins is reduced binding to target sites. Because alteration of a binding site shared by several toxins may cause resistance to all of them, knowledge of which toxins share binding sites is useful for predicting cross-resistance. Conversely, cross-resistance among toxins suggests that the toxins share a binding site. At least two strains of diamondback moth (Plutella xylostella) with resistance to Cry1A toxins and reduced binding of Cry1A toxins have strong cross-resistance to Cry1Ja. Thus, we hypothesized that Cry1Ja shares binding sites with Cry1A toxins. We tested this hypothesis in six moth and butterfly species, each from a different family: Cacyreus marshalli (Lycaenidae), Lobesia botrana (Tortricidae), Manduca sexta (Sphingidae), Pectinophora gossypiella (Gelechiidae), P. xylostella (Plutellidae), and Spodoptera exigua (Noctuidae). Although the extent of competition varied among species, experiments with biotinylated Cry1Ja and radiolabeled Cry1Ac showed that Cry1Ja and Cry1Ac competed for binding sites in all six species. A recent report also indicates shared binding sites for Cry1Ja and Cry1A toxins in Heliothis virescens (Noctuidae). Thus, shared binding sites for Cry1Ja and Cry1A occur in all lepidopteran species tested so far.

  14. Update from the Japanese Center for the Validation of Alternative Methods (JaCVAM).

    PubMed

    Kojima, Hajime

    2013-12-01

    The Japanese Center for the Validation of Alternative Methods (JaCVAM) was established in 2005 to promote the use of alternatives to animal testing in regulatory studies, thereby replacing, reducing, or refining the use of animals, according to the Three Rs principles. JaCVAM assesses the utility, limitations and suitability for use in regulatory studies, of test methods needed to determine the safety of chemicals and other materials. JaCVAM also organises and performs validation studies of new test methods, when necessary. In addition, JaCVAM co-operates and collaborates with similar organisations in related fields, both in Japan and internationally, which also enables JaCVAM to provide input during the establishment of guidelines for new alternative experimental methods. These activities help facilitate application and approval processes for the manufacture and sale of pharmaceuticals, chemicals, pesticides, and other products, as well as for revisions to standards for cosmetic products. In this manner, JaCVAM plays a leadership role in the introduction of new alternative experimental methods for regulatory acceptance in Japan.

  15. Selling energy conservation.

    PubMed

    Hinrichsen, D

    1995-01-01

    This article concerns the Organization of the Petroleum Exporting Countries (OPEC) crisis and its impact on energy efficiency measures in the US. In 1985, when the OPEC collapsed, the US government had avoided the need to construct 350 gigawatts of new electric capacity. The most successful efficiency improvements, especially in household appliances and equipment, lighting and tightened energy efficiency standards in new buildings, resulted from the OPEC event. The real innovation of that time was the change in profit rules for utilities. This revolution and the way some US utilities view energy have not caught on elsewhere. Despite the initiative toward improving energy efficiency in homes, offices and industries, the change has been slow. Partly to blame are the big development banks, which pointed out that short-term conservation and efficiency measures could save at least 15% of the total energy demand without the need for major investment. The benefits of energy conservation was shown during the oil shock when per capita energy consumption fell by 5% in the member states of the Organization of Economic Cooperation and Development, while the per capita gross domestic product grew by a third. There has been a decrease in energy expenditure worldwide, and the scope for further energy savings is enormous, but governments need to recognize and seize the opportunity.

  16. Selling to NASA

    NASA Technical Reports Server (NTRS)

    1986-01-01

    Prospective contractors are acquainted with the organizational structure of NASA, and the major technical program offices and selected staff offices at the Headquarters level are briefly described. The basic procedures for Federal procurement are covered. A primer is presented on how to market to NASA. While the information is specific to NASA, many of the principles are applicable to other agencies as well. Some of the major programs are introduced which are available to small and disadvantaged businesses. The major research programs and fields of interest at individual NASA centers are summarized.

  17. Selling to NASA

    NASA Technical Reports Server (NTRS)

    1990-01-01

    This handbook is designed to promote a better understanding of NASA's interests and the process of doing business with NASA. The document is divided into the following sections: (1) this is NASA; (2) the procurement process; (3) marketing your capabilities; (4) special assistance programs; (5) NASA field installations; (6) sources of additional help; (7) listing of NASA small/minority business personnel; and (8) NASA organization chart.

  18. Selling to NASA

    NASA Technical Reports Server (NTRS)

    1981-01-01

    The prospective NASA contractor is provided with information that describes the agency and its procurement practices. Products include ideas, manufacturing capabilities, fabricated components, construction, basic materials, and specialized services. NASA assistance in marketing these and other products is emphasized. Small and minority business enterprises are discussed. The agency's scientific and technical information activities are also discussed.

  19. Hormone Replacement Therapy advertising: sense and nonsense on the web pages of the best-selling pharmaceuticals in Spain

    PubMed Central

    2010-01-01

    Background The balance of the benefits and risks of long term use of hormone replacement therapy (HRT) have been a matter of debate for decades. In Europe, HRT requires medical prescription and its advertising is only permitted when aimed at health professionals (direct to consumer advertising is allowed in some non European countries). The objective of this study is to analyse the appropriateness and quality of Internet advertising about HRT in Spain. Methods A search was carried out on the Internet (January 2009) using the eight best-selling HRT drugs in Spain. The brand name of each drug was entered into Google's search engine. The web sites appearing on the first page of results and the corresponding companies were analysed using the European Code of Good Practice as the reference point. Results Five corporate web pages: none of them included bibliographic references or measures to ensure that the advertising was only accessible by health professionals. Regarding non-corporate web pages (n = 27): 41% did not include the company name or address, 44% made no distinction between patient and health professional information, 7% contained bibliographic references, 26% provided unspecific information for the use of HRT for osteoporosis and 19% included menstrual cycle regulation or boosting feminity as an indication. Two online pharmacies sold HRT drugs which could be bought online in Spain, did not include the name or contact details of the registered company, nor did they stipulate the need for a medical prescription or differentiate between patient and health professional information. Conclusions Even though pharmaceutical companies have committed themselves to compliance with codes of good practice, deficiencies were observed regarding the identification, information and promotion of HRT medications on their web pages. Unaffected by legislation, non-corporate web pages are an ideal place for indirect HRT advertising, but they often contain misleading information

  20. Expression of a Flax Allene Oxide Synthase cDNA Leads to Increased Endogenous Jasmonic Acid (JA) Levels in Transgenic Potato Plants but Not to a Corresponding Activation of JA-Responding Genes.

    PubMed Central

    Harms, K.; Atzorn, R.; Brash, A.; Kuhn, H.; Wasternack, C.; Willmitzer, L.; Pena-Cortes, H.

    1995-01-01

    Both jasmonic acid (JA) and its methyl ester, methyl jasmonate (MeJA), are thought to be significant components of the signaling pathway regulating the expression of plant defense genes in response to various stresses. JA and MeJA are plant lipid derivatives synthesized from [alpha]-linolenic acid by a lipoxygenase-mediated oxygenation leading to 13-hydroperoxylinolenic acid, which is subsequently transformed by the action of allene oxide synthase (AOS) and additional modification steps. AOS converts lipoxygenase-derived fatty acid hydroperoxide to allene epoxide, which is the precursor for JA formation. Overexpression of flax AOS cDNA under the regulation of the cauliflower mosaic virus 35S promoter in transgenic potato plants led to an increase in the endogenous level of JA. Transgenic plants had six- to 12-fold higher levels of JA than the nontransformed plants. Increased levels of JA have been observed when potato and tomato plants are mechanically wounded. Under these conditions, the proteinase inhibitor II (pin2) genes are expressed in the leaves. Despite the fact that the transgenic plants had levels of JA similar to those found in nontransgenic wounded plants, pin2 genes were not constitutively expressed in the leaves of these plants. Transgenic plants with increased levels of JA did not show changes in water state or in the expression of water stress-responsive genes. Furthermore, the transgenic plants overexpressing the flax AOS gene, and containing elevated levels of JA, responded to wounding or water stress by a further increase in JA and by activating the expression of either wound- or water stress-inducible genes. Protein gel blot analysis demonstrated that the flax-derived AOS protein accumulated in the chloroplasts of the transgenic plants. PMID:12242357

  1. Quali-quantitative analysis of best selling drugs from pharmacy, street market and traditional herbal medicine: a pilot study of market surveillance in Senegal.

    PubMed

    Pichini, Simona; Rotolo, Maria Concetta; Bellotti, Pasquale; Minutillo, Adele; Mastrobattista, Luisa; Pacifici, Roberta

    2015-02-01

    A pilot study of market surveillance in Senegal has been performed analyzing best selling drugs from an official pharmacy and a street market in two principal cities of Senegal and some traditional preparations from herbal medicine from the same market. A simple and rapid gas chromatography method with mass spectrometry detection has been applied after a liquid-liquid extraction of pharmaceutical products and traditional preparations at acidic, neutral and basic pH with chloroform-isopropanol (9:1, v/v). The assay was validated in the range from 10mg to 250 mg/g powder preparations with good determination coefficients (r(2)≥ 0.99) for the calibration curves. At three concentrations spanning the linear dynamic ranges of the calibration curves, mean recoveries of substances under investigation were always higher than 90% and intra-assay and inter-assay precision and accuracy were always better than 15%. The four best selling drugs purchased from a Dakar local pharmacy exactly contained the amount of active principles reported in the respective labels while the best selling drugs freely purchased from Kaolack market contained an amount of active ingredients lower than that declared on the label. No pharmacological active compound, but salicylic acid was found in one of the traditional herbal preparations. This pilot study showed that whereas official drugs sold in pharmacies at prices accessible for a very few portion of the population contained the amount of active principles as reported in the labels, those from street market bought by the majority of population contained an amount of active ingredients lower than that declared on the label and finally traditional herbal preparations seldom contain pharmacological active principles.

  2. Can the government ban organ sale? Recent court challenges and the future of US law on selling human organs and other tissue.

    PubMed

    Cohen, I G

    2012-08-01

    On December 1, 2011, in Flynn v. Holder, a panel of the US Court of Appeals for the Ninth Circuit upheld the National Organ Transplant Act of 1984 (NOTA) from a constitutional challenge, but interpreted the act such that its prohibition on sale did not encompass "peripheral blood stem cells" obtained through apheresis. Rehearing of the case was denied on March 27, 2012. The Obama administration must now decide whether to pursue its challenge in the US Supreme Court. This article explains the litigation, its significance and uses it as a backdrop against which to understand the history and future trajectory of the laws governing selling organs and other human tissue.

  3. Optimal functional levels of activation-induced deaminase specifically require the Hsp40 DnaJa1

    PubMed Central

    Orthwein, Alexandre; Zahn, Astrid; Methot, Stephen P; Godin, David; Conticello, Silvestro G; Terada, Kazutoyo; Di Noia, Javier M

    2012-01-01

    The enzyme activation-induced deaminase (AID) deaminates deoxycytidine at the immunoglobulin genes, thereby initiating antibody affinity maturation and isotype class switching during immune responses. In contrast, off-target DNA damage caused by AID is oncogenic. Central to balancing immunity and cancer is AID regulation, including the mechanisms determining AID protein levels. We describe a specific functional interaction between AID and the Hsp40 DnaJa1, which provides insight into the function of both proteins. Although both major cytoplasmic type I Hsp40s, DnaJa1 and DnaJa2, are induced upon B-cell activation and interact with AID in vitro, only DnaJa1 overexpression increases AID levels and biological activity in cell lines. Conversely, DnaJa1, but not DnaJa2, depletion reduces AID levels, stability and isotype switching. In vivo, DnaJa1-deficient mice display compromised response to immunization, AID protein and isotype switching levels being reduced by half. Moreover, DnaJa1 farnesylation is required to maintain, and farnesyltransferase inhibition reduces, AID protein levels in B cells. Thus, DnaJa1 is a limiting factor that plays a non-redundant role in the functional stabilization of AID. PMID:22085931

  4. A balanced JA/ABA status may correlate with adaptation to osmotic stress in Vitis cells.

    PubMed

    Ismail, Ahmed; Seo, Mitsunori; Takebayashi, Yumiko; Kamiya, Yuji; Nick, Peter

    2015-08-01

    Water-related stress is considered a major type of plant stress. Osmotic stress, in particular, represents the common part of all water-related stresses. Therefore, plants have evolved different adaptive mechanisms to cope with osmotic-related disturbances. In the current work, two grapevine cell lines that differ in their osmotic adaptability, Vitis rupestris and Vitis riparia, were investigated under mannitol-induced osmotic stress. To dissect signals that lead to adaptability from those related to sensitivity, osmotic-triggered responses with respect to jasmonic acid (JA) and its active form JA-Ile, abscisic acid (ABA), and stilbene compounds, as well as the expression of their related genes were observed. In addition, the transcript levels of the cellular homeostasis gene NHX1 were examined. The data are discussed with a hypothesis suggesting that a balance of JA and ABA status might correlate with cellular responses, either guiding cells to sensitivity or to progress toward adaptation.

  5. Effect of 25% Sodium Reduction on Sales of a Top-Selling Bread in Remote Indigenous Australian Community Stores: A Controlled Intervention Trial

    PubMed Central

    McMahon, Emma; Webster, Jacqui; Brimblecombe, Julie

    2017-01-01

    Reducing sodium in the food supply is key to achieving population salt targets, but maintaining sales is important to ensuring commercial viability and maximising clinical impact. We investigated whether 25% sodium reduction in a top-selling bread affected sales in 26 remote Indigenous community stores. After a 23-week baseline period, 11 control stores received the regular-salt bread (400 mg Na/100 g) and 15 intervention stores received the reduced-salt version (300 mg Na/100 g) for 12-weeks. Sales data were collected to examine difference between groups in change from baseline to follow-up (effect size) in sales (primary outcome) or sodium density, analysed using a mixed model. There was no significant effect on market share (−0.31%; 95% CI −0.68, 0.07; p = 0.11) or weekly dollars ($58; −149, 266; p = 0.58). Sodium density of all purchases was not significantly reduced (−8 mg Na/MJ; −18, 2; p = 0.14), but 25% reduction across all bread could significantly reduce sodium (−12; −23, −1; p = 0.03). We found 25% salt reduction in a top-selling bread did not affect sales in remote Indigenous community stores. If achieved across all breads, estimated salt intake in remote Indigenous Australian communities would be reduced by approximately 15% of the magnitude needed to achieve population salt targets, which could lead to significant health gains at the population-level. PMID:28264485

  6. Integrated metabolomic and proteomic analysis reveals systemic responses of Rubrivivax benzoatilyticus JA2 to aniline stress.

    PubMed

    Mujahid, Md; Prasuna, M Lakshmi; Sasikala, Ch; Ramana, Ch Venkata

    2015-02-06

    Aromatic amines are widely distributed in the environment and are major environmental pollutants. Although degradation of aromatic amines is well studied in bacteria, physiological adaptations and stress response to these toxic compounds is not yet fully understood. In the present study, systemic responses of Rubrivivax benzoatilyticus JA2 to aniline stress were deciphered using metabolite and iTRAQ-labeled protein profiling. Strain JA2 tolerated high concentrations of aniline (30 mM) with trace amounts of aniline being transformed to acetanilide. GC-MS metabolite profiling revealed aniline stress phenotype wherein amino acid, carbohydrate, fatty acid, nitrogen metabolisms, and TCA (tricarboxylic acid cycle) were modulated. Strain JA2 responded to aniline by remodeling the proteome, and cellular functions, such as signaling, transcription, translation, stress tolerance, transport and carbohydrate metabolism, were highly modulated. Key adaptive responses, such as transcription/translational changes, molecular chaperones to control protein folding, and efflux pumps implicated in solvent extrusion, were induced in response to aniline stress. Proteo-metabolomics indicated extensive rewiring of metabolism to aniline. TCA cycle and amino acid catabolism were down-regulated while gluconeogenesis and pentose phosphate pathways were up-regulated, leading to the synthesis of extracellular polymeric substances. Furthermore, increased saturated fatty acid ratios in membranes due to aniline stress suggest membrane adaptation. The present study thus indicates that strain JA2 employs multilayered responses: stress response, toxic compound tolerance, energy conservation, and metabolic rearrangements to aniline.

  7. Transcriptome Analysis in Haematococcus pluvialis: Astaxanthin Induction by Salicylic Acid (SA) and Jasmonic Acid (JA).

    PubMed

    Gao, Zhengquan; Li, Yan; Wu, Guanxun; Li, Guoqiang; Sun, Haifeng; Deng, Suzhen; Shen, Yicheng; Chen, Guoqiang; Zhang, Ruihao; Meng, Chunxiao; Zhang, Xiaowen

    2015-01-01

    Haematococcus pluvialis is an astaxanthin-rich microalga that can increase its astaxanthin production by salicylic acid (SA) or jasmonic acid (JA) induction. The genetic transcriptome details of astaxanthin biosynthesis were analyzed by exposing the algal cells to 25 mg/L of SA and JA for 1, 6 and 24 hours, plus to the control (no stress). Based on the RNA-seq analysis, 56,077 unigenes (51.7%) were identified with functions in response to the hormone stress. The top five identified subcategories were cell, cellular process, intracellular, catalytic activity and cytoplasm, which possessed 5600 (~9.99%), 5302 (~9.45%), 5242 (~9.35%), 4407 (~7.86%) and 4195 (~7.48%) unigenes, respectively. Furthermore, 59 unigenes were identified and assigned to 26 putative transcription factors (TFs), including 12 plant-specific TFs. They were likely associated with astaxanthin biosynthesis in Haematococcus upon SA and JA stress. In comparison, the up-regulation of differential expressed genes occurred much earlier, with higher transcript levels in the JA treatment (about 6 h later) than in the SA treatment (beyond 24 h). These results provide valuable information for directing metabolic engineering efforts to improve astaxanthin biosynthesis in H. pluvialis.

  8. Why California retailers stop selling tobacco products, and what their customers and employees think about it when they do: case studies

    PubMed Central

    2011-01-01

    Background In California, some 40, 000 retailers sell tobacco products. Tobacco's ubiquitousness in retail settings normalizes use and cues smoking urges among former smokers and those attempting cessation. Thus, limiting the number of retailers is regarded as key to ending the tobacco epidemic. In the past decade, independent pharmacies and local grocery chains in California and elsewhere have voluntarily abandoned tobacco sales. No previous studies have examined the reasons for this emerging phenomenon. We sought to learn what motivated retailers to discontinue tobacco sales and what employees and customers thought about their decision. Methods We conducted case studies of seven California retailers (three grocery stores, four pharmacies) that had voluntarily ceased tobacco sales within the past 7 years. We interviewed owners, managers, and employees, conducted consumer focus groups, unobtrusively observed businesses and the surrounding environment, and examined any media coverage of each retailer's decision. We analyzed data using qualitative content analysis. Results For independent pharmacies, the only reason given for the decision to end tobacco sales was that tobacco caused disease and death. Grocers listed health among several factors, including regulatory pressures and wanting to be seen as "making a difference." Media coverage of stores' new policies was limited, and only three retailers alerted customers. Management reported few or no customer complaints and supportive or indifferent employees. Pharmacy employees were pleased to no longer be selling a deadly product. Grocery store management saw the decision to end tobacco sales as enhancing the stores' image and consistent with their inventory of healthy foods. Focus group participants (smokers and nonsmokers) were largely unaware that retailers had stopped selling tobacco; however, almost all supported the decision, viewing it as promoting public health. Many said knowing this made them more likely to

  9. Partial Activation of SA- and JA-Defensive Pathways in Strawberry upon Colletotrichum acutatum Interaction

    PubMed Central

    Amil-Ruiz, Francisco; Garrido-Gala, José; Gadea, José; Blanco-Portales, Rosario; Muñoz-Mérida, Antonio; Trelles, Oswaldo; de los Santos, Berta; Arroyo, Francisco T.; Aguado-Puig, Ana; Romero, Fernando; Mercado, José-Ángel; Pliego-Alfaro, Fernando; Muñoz-Blanco, Juan; Caballero, José L.

    2016-01-01

    Understanding the nature of pathogen host interaction may help improve strawberry (Fragaria × ananassa) cultivars. Plant resistance to pathogenic agents usually operates through a complex network of defense mechanisms mediated by a diverse array of signaling molecules. In strawberry, resistance to a variety of pathogens has been reported to be mostly polygenic and quantitatively inherited, making it difficult to associate molecular markers with disease resistance genes. Colletotrichum acutatum spp. is a major strawberry pathogen, and completely resistant cultivars have not been reported. Moreover, strawberry defense network components and mechanisms remain largely unknown and poorly understood. Assessment of the strawberry response to C. acutatum included a global transcript analysis, and acidic hormones SA and JA measurements were analyzed after challenge with the pathogen. Induction of transcripts corresponding to the SA and JA signaling pathways and key genes controlling major steps within these defense pathways was detected. Accordingly, SA and JA accumulated in strawberry after infection. Contrastingly, induction of several important SA, JA, and oxidative stress-responsive defense genes, including FaPR1-1, FaLOX2, FaJAR1, FaPDF1, and FaGST1, was not detected, which suggests that specific branches in these defense pathways (those leading to FaPR1-2, FaPR2-1, FaPR2-2, FaAOS, FaPR5, and FaPR10) were activated. Our results reveal that specific aspects in SA and JA dependent signaling pathways are activated in strawberry upon interaction with C. acutatum. Certain described defense-associated transcripts related to these two known signaling pathways do not increase in abundance following infection. This finding suggests new insight into a specific putative molecular strategy for defense against this pathogen. PMID:27471515

  10. Partial Activation of SA- and JA-Defensive Pathways in Strawberry upon Colletotrichum acutatum Interaction.

    PubMed

    Amil-Ruiz, Francisco; Garrido-Gala, José; Gadea, José; Blanco-Portales, Rosario; Muñoz-Mérida, Antonio; Trelles, Oswaldo; de Los Santos, Berta; Arroyo, Francisco T; Aguado-Puig, Ana; Romero, Fernando; Mercado, José-Ángel; Pliego-Alfaro, Fernando; Muñoz-Blanco, Juan; Caballero, José L

    2016-01-01

    Understanding the nature of pathogen host interaction may help improve strawberry (Fragaria × ananassa) cultivars. Plant resistance to pathogenic agents usually operates through a complex network of defense mechanisms mediated by a diverse array of signaling molecules. In strawberry, resistance to a variety of pathogens has been reported to be mostly polygenic and quantitatively inherited, making it difficult to associate molecular markers with disease resistance genes. Colletotrichum acutatum spp. is a major strawberry pathogen, and completely resistant cultivars have not been reported. Moreover, strawberry defense network components and mechanisms remain largely unknown and poorly understood. Assessment of the strawberry response to C. acutatum included a global transcript analysis, and acidic hormones SA and JA measurements were analyzed after challenge with the pathogen. Induction of transcripts corresponding to the SA and JA signaling pathways and key genes controlling major steps within these defense pathways was detected. Accordingly, SA and JA accumulated in strawberry after infection. Contrastingly, induction of several important SA, JA, and oxidative stress-responsive defense genes, including FaPR1-1, FaLOX2, FaJAR1, FaPDF1, and FaGST1, was not detected, which suggests that specific branches in these defense pathways (those leading to FaPR1-2, FaPR2-1, FaPR2-2, FaAOS, FaPR5, and FaPR10) were activated. Our results reveal that specific aspects in SA and JA dependent signaling pathways are activated in strawberry upon interaction with C. acutatum. Certain described defense-associated transcripts related to these two known signaling pathways do not increase in abundance following infection. This finding suggests new insight into a specific putative molecular strategy for defense against this pathogen.

  11. Gnawing Pains, Festering Ulcers, and Nightmare Suffering: Selling Leprosy as a Humanitarian Cause in the British Empire, c. 1890-1960

    PubMed Central

    Vongsathorn, Kathleen

    2014-01-01

    When British attention was drawn to the issue of leprosy in the Empire, humanitarian organisations rose to take on responsibility for the ‘fight against leprosy’. In an effort to fundraise for a distant cause at a time when hundreds of charities competed for the financial support of British citizens, fundraisers developed propaganda to set leprosy apart from all other humanitarian causes. They drew on leprosy’s relationship with Christianity, its debilitating symptoms, and the supposed vulnerability of leprosy sufferers in order to mobilise Britain’s sense of humanitarian, Christian, and patriotic duty. This article traces the emergence of leprosy as a popular imperial humanitarian cause in modern Britain and analyses the narratives of religion, suffering, and disease that they created and employed in order to fuel their growth and sell leprosy as a British humanitarian cause. PMID:24932060

  12. How to sell a condom? The impact of demand creation tools on male and female condom sales in resource limited settings.

    PubMed

    Terris-Prestholt, Fern; Windmeijer, Frank

    2016-07-01

    Despite condoms being cheap and effective in preventing HIV, there remains an 8billion shortfall in condom use in risky sex-acts. Social marketing organisations apply private sector marketing approaches to sell public health products. This paper investigates the impact of marketing tools, including promotion and pricing, on demand for male and female condoms in 52 countries between 1997 and 2009. A static model differentiates drivers of demand between products, while a dynamic panel data estimator estimates their short- and long-run impacts. Products are not equally affected: female condoms are not affected by advertising, but highly affected by interpersonal communication and HIV prevalence. Price and promotion have significant short- and long-run effects, with female condoms far more sensitive to price than male condoms. The design of optimal distribution strategies for new and existing HIV prevention technologies must consider both product and target population characteristics.

  13. Physiological Characteristics and Production of Folic Acid of Lactobacillus plantarum JA71 Isolated from Jeotgal, a Traditional Korean Fermented Seafood

    PubMed Central

    Lim, Sang-Dong

    2014-01-01

    Folic acid, one of the B group of vitamins, is an essential substance for maintaining the functions of the nervous system, and is also known to decrease the level of homocysteine in plasma. Homocysteine influences the lowering of the cognitive function in humans, and especially in elderly people. In order to determine the strains with a strong capacity to produce folic acid, 190 bacteria were isolated from various kinds of jeotgal and chungkuk-jang. In our test experiment, JA71 was found to contain 9.03μg/mL of folic acid after 24 h of incubation in an MRS broth. This showed that JA71 has the highest folic acid production ability compared to the other lactic acid bacteria that were isolated. JA71 was identified as Lactobacillus plantarum by the result of API carbohydrate fermentation pattern and 16s rDNA sequence. JA71 was investigated for its physiological characteristics. The optimum growth temperature of JA71 was 37℃, and the cultures took 12 h to reach pH 4.4. JA71 proved more sensitive to bacitracin when compared with fifteen different antibiotics, and showed most resistance to neomycin and vancomycin. Moreover, it was comparatively tolerant of bile juice and acid, and displayed resistance to Escherichia coli, Salmonella Typhimurium, and Staphylococcus aureus with restraint rates of 60.4%, 96.7%, and 76.2%, respectively. These results demonstrate that JA71 could be an excellent strain for application to functional products. PMID:26760752

  14. New Enhanced Artificial Bee Colony (JA-ABC5) Algorithm with Application for Reactive Power Optimization

    PubMed Central

    2015-01-01

    The standard artificial bee colony (ABC) algorithm involves exploration and exploitation processes which need to be balanced for enhanced performance. This paper proposes a new modified ABC algorithm named JA-ABC5 to enhance convergence speed and improve the ability to reach the global optimum by balancing exploration and exploitation processes. New stages have been proposed at the earlier stages of the algorithm to increase the exploitation process. Besides that, modified mutation equations have also been introduced in the employed and onlooker-bees phases to balance the two processes. The performance of JA-ABC5 has been analyzed on 27 commonly used benchmark functions and tested to optimize the reactive power optimization problem. The performance results have clearly shown that the newly proposed algorithm has outperformed other compared algorithms in terms of convergence speed and global optimum achievement. PMID:25879054

  15. Aniline is an inducer, and not a precursor, for indole derivatives in Rubrivivax benzoatilyticus JA2.

    PubMed

    Mujahid, Mohammed; Sasikala, Ch; Ramana, Ch V

    2014-01-01

    Rubrivivax benzoatilyticus JA2 and other anoxygenic photosynthetic bacteria produce indole derivatives when exposed to aniline, a xenobiotic compound. Though this phenomenon has been reported previously, the role of aniline in the production of indoles is still a biochemical riddle. The present study aims at understanding the specific role of aniline (as precursor or stimulator) in the production of indoles and elucidating the biochemical pathway of indoles in aniline-exposed cells by using stable isotope approaches. Metabolic profiling revealed tryptophan accumulation only in aniline exposed cells along with indole 3-acetic acid (IAA) and indole 3-aldehyde (IAld), the two major catabolites of tryptophan. Deuterium labelled aniline feeding studies revealed that aniline is not a precursor of indoles in strain JA2. Further, production of indoles only in aniline-exposed cells suggests that aniline is an indoles stimulator. In addition, production of indoles depended on the presence of a carbon source, and production enhanced when carbon sources were added to the culture. Isotope labelled fumarate feeding identified, fumarate as the precursor of indole, indicating de novo synthesis of indoles. Glyphosate (shikimate pathway inhibitor) inhibited the indoles production, accumulation of tryptophan, IAA and IAld indicating that indoles synthesis in strain JA2 occurs via the de novo shikimate pathway. The up-regulation of anthranilate synthase gene and induction of anthranilate synthase activity correlated well with tryptophan production in strain JA2. Induction of tryptophan aminotransferase and tryptophan 2-monooxygenase activities corroborated well with IAA levels, suggesting that tryptophan catabolism occurs simultaneously in aniline exposed cells. Our study demonstrates that aniline (stress) stimulates tryptophan/indoles synthesis via the shikimate pathway by possibly modulating the metabolic pathway.

  16. Aniline Is an Inducer, and Not a Precursor, for Indole Derivatives in Rubrivivax benzoatilyticus JA2

    PubMed Central

    Mohammed, Mujahid; Ch, Sasikala; Ch, Ramana V.

    2014-01-01

    Rubrivivax benzoatilyticus JA2 and other anoxygenic photosynthetic bacteria produce indole derivatives when exposed to aniline, a xenobiotic compound. Though this phenomenon has been reported previously, the role of aniline in the production of indoles is still a biochemical riddle. The present study aims at understanding the specific role of aniline (as precursor or stimulator) in the production of indoles and elucidating the biochemical pathway of indoles in aniline-exposed cells by using stable isotope approaches. Metabolic profiling revealed tryptophan accumulation only in aniline exposed cells along with indole 3-acetic acid (IAA) and indole 3-aldehyde (IAld), the two major catabolites of tryptophan. Deuterium labelled aniline feeding studies revealed that aniline is not a precursor of indoles in strain JA2. Further, production of indoles only in aniline-exposed cells suggests that aniline is an indoles stimulator. In addition, production of indoles depended on the presence of a carbon source, and production enhanced when carbon sources were added to the culture. Isotope labelled fumarate feeding identified, fumarate as the precursor of indole, indicating de novo synthesis of indoles. Glyphosate (shikimate pathway inhibitor) inhibited the indoles production, accumulation of tryptophan, IAA and IAld indicating that indoles synthesis in strain JA2 occurs via the de novo shikimate pathway. The up-regulation of anthranilate synthase gene and induction of anthranilate synthase activity correlated well with tryptophan production in strain JA2. Induction of tryptophan aminotransferase and tryptophan 2-monooxygenase activities corroborated well with IAA levels, suggesting that tryptophan catabolism occurs simultaneously in aniline exposed cells. Our study demonstrates that aniline (stress) stimulates tryptophan/indoles synthesis via the shikimate pathway by possibly modulating the metabolic pathway. PMID:24533057

  17. Biosynthesis of silver and zinc oxide nanoparticles using Pichia fermentans JA2 and their antimicrobial property

    NASA Astrophysics Data System (ADS)

    Chauhan, Ritika; Reddy, Arpita; Abraham, Jayanthi

    2015-01-01

    The development of eco-friendly alternative to chemical synthesis of metal nanoparticles is of great challenge among researchers. The present study aimed to investigate the biological synthesis, characterization, antimicrobial study and synergistic effect of silver and zinc oxide nanoparticles against clinical pathogens using Pichia fermentans JA2. The extracellular biosynthesis of silver and zinc oxide nanoparticles was investigated using Pichia fermentans JA2 isolated from spoiled fruit pulp bought in Vellore local market. The crystalline and stable metallic nanoparticles were characterized evolving several analytical techniques including UV-visible spectrophotometer, X-ray diffraction pattern analysis and FE-scanning electron microscope with EDX-analysis. The biosynthesized metallic nanoparticles were tested for their antimicrobial property against medically important Gram positive, Gram negative and fungal pathogenic microorganisms. Furthermore, the biosynthesized nanoparticles were also evaluated for their increased antimicrobial activities with various commercially available antibiotics against clinical pathogens. The biosynthesized silver nanoparticles inhibited most of the Gram negative clinical pathogens, whereas zinc oxide nanoparticles were able to inhibit only Pseudomonas aeruginosa. The combined effect of standard antibiotic disc and biosynthesized metallic nanoparticles enhanced the inhibitory effect against clinical pathogens. The biological synthesis of silver and zinc oxide nanoparticles is a novel and cost-effective approach over harmful chemical synthesis techniques. The metallic nanoparticles synthesized using Pichia fermentans JA2 possess potent inhibitory effect that offers valuable contribution to pharmaceutical associations.

  18. The crosstalk between Target of Rapamycin (TOR) and Jasmonic Acid (JA) signaling existing in Arabidopsis and cotton

    PubMed Central

    Song, Yun; Zhao, Ge; Zhang, Xueyan; Li, Linxuan; Xiong, Fangjie; Zhuo, Fengping; Zhang, Chaojun; Yang, Zuoren; Datla, Raju; Ren, Maozhi; Li, Fuguang

    2017-01-01

    Target of rapamycin (TOR) acts as an important regulator of cell growth, development and stress responses in most examined diploid eukaryotes. However, little is known about TOR in tetraploid species such as cotton. Here, we show that TORC1-S6K-RPS6, the major signaling components, are conserved and further expanded in cotton genome. Though the cotton seedlings are insensitive to rapamycin, AZD8055, the second-generation inhibitor of TOR, can significantly suppress the growth in cotton. Global transcriptome analysis revealed that genes associated with jasmonic acid (JA) biosynthesis and transduction were significantly altered in AZD8055 treated cotton seedlings, suggesting the potential crosstalk between TOR and JA signaling. Pharmacological and genetic approaches have been employed to get further insights into the molecular mechanism of the crosstalk between TOR and JA. Combination of AZD8055 with methyl jasmonate can synergistically inhibit cotton growth, and additionally JA levels were significantly increased when cotton seedlings were subjected to AZD8055. JA biosynthetic and signaling mutants including jar1, coi1-2 and myc2-2 displayed TOR inhibitor-resistant phenotypes, whereas COI1 overexpression transgenic lines and jaz10 exhibited sensitivity to AZD8055. Consistently, cotton JAZ can partially rescue TOR-suppressed phenotypes in Arabidopsis. These evidences revealed that the crosstalk between TOR and JA pathway operates in cotton and Arabidopsis. PMID:28374843

  19. Medico-legal considerations of canine leishmaniosis in Italy: an overview of an emerging disease with reference to the buying and selling of dogs.

    PubMed

    Passantino, A

    2006-12-01

    Leishmaniosis is a common infection in the canine population. Domestic dogs are the main reservoir hosts for zoonotic human visceral leishmaniosis in both the Old and New Worlds. In fact, canine leishmaniosis is not just a veterinary problem. In the light of some recent advances in the field of diagnosis, the author evaluated the medico-legal aspects of buying and selling dogs that are potentially affected by leishmaniosis. This paper clarifies why it is impossible to attribute redhibitory vice (an essential defect in a product which renders it useless or so diminishes its usefulness or value that it must be presumed that, if he had been aware of it, the buyer would not have bought it, or would have paid a lesser price) to this disease and highlights the necessity to improve Sicilian regional law no. 15 of 3 July 2000 concerning the creation of a dog register by giving every animal a health book. With this aim in mind, the author suggests that leishmaniosis be included in the list of notifiable diseases that appears in article 1 of the Italian Veterinary Police Regulations so as to reduce the possibilities of fraudulent trade in dogs known to be infected before purchase.

  20. Priming for JA-dependent defenses using hexanoic acid is an effective mechanism to protect Arabidopsis against B. cinerea.

    PubMed

    Kravchuk, Zhana; Vicedo, Begonya; Flors, Víctor; Camañes, Gemma; González-Bosch, Carmen; García-Agustín, Pilar

    2011-03-01

    Soil drench treatments with hexanoic acid can effectively protect Arabidopsis plants against Botrytis cinerea through a mechanism based on a stronger and faster accumulation of JA-dependent defenses. Plants impaired in ethylene, salicylic acid, abscisic acid or glutathion pathways showed intact protection by hexanoic acid upon B. cinerea infection. Accordingly, no significant changes in the SA marker gene PR-1 in either the SA or ABA hormone balance were observed in the infected and treated plants. In contrast, the JA signaling pathway showed dramatic changes after hexanoic acid treatment, mainly when the pathogen was present. The impaired JA mutants, jin1-2 and jar1, were unable to display hexanoic acid priming against the necrotroph. In addition, hexanoic acid-treated plants infected with B. cinerea showed priming in the expression of the PDF1.2, PR-4 and VSP1 genes implicated in the JA pathways. Moreover, JA and OPDA levels were primed at early stages by hexanoic acid. Treatments also stimulated increased callose accumulation in response to the pathogen. Although callose accumulation has proved an effective IR mechanism against B. cinerea, it is apparently not essential to express hexanoic acid-induced resistance (HxAc-IR) because the mutant pmr4.1 (callose synthesis defective mutant) is protected by treatment. We recently described how hexanoic acid treatments can protect tomato plants against B. cinerea by stimulating ABA-dependent callose deposition and by priming OPDA and JA-Ile production. We clearly demonstrate here that Hx-IR is a dependent plant species, since this acid protects Arabidopsis plants against the same necrotroph by priming JA-dependent defenses without enhancing callose accumulation.

  1. Rice Rab11 is required for JA-mediated defense signaling

    SciTech Connect

    Hong, Min Ji; Lee, Yun mi; Son, Young Sim; Im, Chak Han; Yi, Young Byung; Rim, Yeong Gil; Bahk, Jeong Dong; Heo, Jae Bok

    2013-05-17

    Highlights: •OsRab11 interacts with OsOPR8. •OsOPR8 is localized in the cytosol and peroxisome. •OsRab11 enhances the NADPH consumption by OsOPR8. •Transgenic Arabidopsis overexpressing OsRab11 represents a pathogen-resistant phenotype. -- Abstract: Rab proteins play an essential role in regulating vesicular transport in eukaryotic cells. Previously, we characterized OsRab11, which in concert with OsGAP1 and OsGDI3 regulates vesicular trafficking from the trans-Golgi network (TGN) to the plasma membrane or vacuole. To further elucidate the physiological function of OsRab11 in plants, we performed yeast two-hybrid screens using OsRab11 as bait. OsOPR8 was isolated and shown to interact with OsRab11. A co-immunoprecipitation assay confirmed this interaction. The green fluorescent protein-OsOPR8 fusion product was targeted to the cytoplasm and peroxisomes of protoplasts from Arabidopsis thaliana. OsOPR8 exhibited NADPH-dependent reduction activity when 2-cyclohexen-1-one (CyHE) and 12-oxo-phytodienoic acid (OPDA) were supplied as possible substrates. Interestingly, NADPH oxidation by OsOPR8 was increased when wild-type OsRab11 or the constitutively active form of OsRab11 (Q78L) were included in the reaction mix, but not when the dominant negative form of OsRab11 (S28N) was included. OsRab11 was expressed broadly in plants and both OsRab11 and OsOPR8 were induced by jasmonic acid (JA) and elicitor treatments. Overexpressed OsRab11 transgenic plants showed resistance to pathogens through induced expression of JA-responsive genes. In conclusion, OsRab11 may be required for JA-mediated defense signaling by activating the reducing activity of OsOPR8.

  2. MAPK-dependent JA and SA signalling in Nicotiana attenuata affects plant growth and fitness during competition with conspecifics

    PubMed Central

    2012-01-01

    Background Induced defense responses to herbivores are generally believed to have evolved as cost-saving strategies that defer the fitness costs of defense metabolism until these defenses are needed. The fitness costs of jasmonate (JA)-mediated defenses have been well documented. Those of the early signaling units mediating induced resistance to herbivores have yet to be examined. Early signaling components that mediate herbivore-induced defense responses in Nicotiana attenuata, have been well characterized and here we examine their growth and fitness costs during competition with conspecifics. Two mitogen-activated protein kinases (MAPKs), salicylic acid (SA)-induced protein kinase (SIPK) and wound-induced protein kinase (WIPK) are rapidly activated after perception of herbivory and both kinases regulate herbivory-induced JA levels and JA-mediated defense metabolite accumulations. Since JA-induced defenses result in resource-based trade-offs that compromise plant productivity, we evaluated if silencing SIPK (irSIPK) and WIPK (irWIPK) benefits the growth and fitness of plants competiting with wild type (WT) plants, as has been shown for plants silenced in JA-signaling by the reduction of Lipoxygenase 3 (LOX3) levels. Results As expected, irWIPK and LOX3-silenced plants out-performed their competing WT plants. Surprisingly, irSIPK plants, which have the largest reductions in JA signaling, did not. Phytohormone profiling of leaves revealed that irSIPK plants accumulated higher levels of SA compared to WT. To test the hypothesis that these high levels of SA, and their presumed associated fitness costs of pathogen associated defenses in irSIPK plants had nullified the JA-deficiency-mediated growth benefits in these plants, we genetically reduced SA levels in irSIPK plants. Reducing SA levels partially recovered the biomass and fitness deficits of irSIPK plants. We also evaluated whether the increased fitness of plants with reduced SA or JA levels resulted from

  3. The tomato res mutant which accumulates JA in roots in non-stressed conditions restores cell structure alterations under salinity.

    PubMed

    Garcia-Abellan, José O; Fernandez-Garcia, Nieves; Lopez-Berenguer, Carmen; Egea, Isabel; Flores, Francisco B; Angosto, Trinidad; Capel, Juan; Lozano, Rafael; Pineda, Benito; Moreno, Vicente; Olmos, Enrique; Bolarin, Maria C

    2015-11-01

    Jasmonic acid (JA) regulates a wide spectrum of plant biological processes, from plant development to stress defense responses. The role of JA in plant response to salt stress is scarcely known, and even less known is the specific response in root, the main plant organ responsible for ionic uptake and transport to the shoot. Here we report the characterization of the first tomato (Solanum lycopersicum) mutant, named res (restored cell structure by salinity), that accumulates JA in roots prior to exposure to stress. The res tomato mutant presented remarkable growth inhibition and displayed important morphological alterations and cellular disorganization in roots and leaves under control conditions, while these alterations disappeared when the res mutant plants were grown under salt stress. Reciprocal grafting between res and wild type (WT) (tomato cv. Moneymaker) indicated that the main organ responsible for the development of alterations was the root. The JA-signaling pathway is activated in res roots prior to stress, with transcripts levels being even higher in control condition than in salinity. Future studies on this mutant will provide significant advances in the knowledge of JA role in root in salt-stress tolerance response, as well as in the energy trade-off between plant growth and response to stress.

  4. OsJAR1 is required for JA-regulated floret opening and anther dehiscence in rice.

    PubMed

    Xiao, Yuguo; Chen, Yi; Charnikhova, Tatsiana; Mulder, Patrick P J; Heijmans, Jeroen; Hoogenboom, Angela; Agalou, Adamantia; Michel, Corinne; Morel, Jean-Benoit; Dreni, Ludovico; Kater, Martin M; Bouwmeester, Harro; Wang, Mei; Zhu, Zhen; Ouwerkerk, Pieter B F

    2014-09-01

    Jasmonates are important phytohormones regulating reproductive development. We used two recessive rice Tos17 alleles of OsJAR1, osjar1-2 and osjar1-3, to study the biological function of jasmonates in rice anthesis. The florets of both osjar1 alleles stayed open during anthesis because the lodicules, which control flower opening in rice, were not withering on time. Furthermore, dehiscence of the anthers filled with viable pollen, was impaired, resulting in lower fertility. In situ hybridization and promoter GUS transgenic analysis confirmed OsJAR1 expression in these floral tissues. Flower opening induced by exogenous applied methyl jasmonate was impaired in osjar1 plants and was restored in a complementation experiment with transgenics expressing a wild type copy of OsJAR1 controlled by a rice actin promoter. Biochemical analysis showed that OsJAR1 encoded an enzyme conjugating jasmonic acid (JA) to at least Ile, Leu, Met, Phe, Trp and Val and both osjar1 alleles had substantial reduction in content of JA-Ile, JA-Leu and JA-Val in florets. We conclude that OsJAR1 is a JA-amino acid synthetase that is required for optimal flower opening and closing and anther dehiscence in rice.

  5. Diversity of lactic acid bacteria from modified atmosphere packaged sliced cooked meat products at sell-by date assessed by PCR-denaturing gradient gel electrophoresis.

    PubMed

    Audenaert, Kris; D'Haene, Klaas; Messens, Kathy; Ruyssen, Tony; Vandamme, Peter; Huys, Geert

    2010-02-01

    The predominant lactic acid bacteria (LAB) microbiota associated with three types of modified atmosphere packaged (MAP) sliced cooked meat products (i.e. ham, turkey and chicken) was analyzed at sell-by date using a combination of culturing and molecular population fingerprinting. Likewise routine analyses during industrial MAP production, meat samples were plated on the general heterotrophic Plate Count Agar (PCA) and on the LAB-specific de Man, Rogosa, Sharpe (MRS) agar under different temperature and atmosphere conditions. Subsequently, community DNA extracts were prepared from culturable bacterial fractions harvested from both media and used for PCR targeting the V3 hyper-variable region of the 16S rRNA gene followed by denaturing gradient gel electrophoresis (DGGE) of PCR amplicons (PCR-DGGE). Irrespective of aerobic or anaerobic incubation conditions, V3-16S rDNA DGGE fingerprints of culturable fractions from PCA and MRS medium displayed a high level of similarity indicating that LAB constituted the most dominant group in the culturable bacterial community. Comparison of DGGE profiles of fractions grown at 20, 28 or 37 degrees C indicated that part of the culturable community consisted of psychrotrophs. Four DGGE bands were common among cooked ham, turkey and chicken products, suggesting that these represent the microbiota circulating in the plant where all three MAP product types were sliced and packaged. Based on band sequencing and band position analysis using LAB reference strains, these four bands could be assigned to Lactobacillus sakei and/or the closely related Lactobacillus fuchuensis, Lactobacillus curvatus, Carnobacterium divergens and Leuconostoc carnosum. In conclusion, the PCR-DGGE approach described in this study allows to discriminate, identify and monitor core and occasional LAB microbiota of MAP sliced cooked meat products and provides valuable complementary information to the current plating procedures routinely used in industrial plants.

  6. Do Sex and Violence Sell? A Meta-Analytic Review of the Effects of Sexual and Violent Media and Ad Content on Memory, Attitudes, and Buying Intentions.

    PubMed

    Lull, Robert B; Bushman, Brad J

    2015-09-01

    It is commonly assumed that sex and violence sell. However, we predicted that sex and violence would have the opposite effect. We based our predictions on the evolution and emotional arousal theoretical framework, which states that people are evolutionarily predisposed to attend to emotionally arousing cues such as sex and violence. Thus, sexual and violent cues demand more cognitive resources than nonsexual and nonviolent cues. Using this framework, we meta-analyzed the effects of sexual media, violent media, sexual ads, and violent ads on the advertising outcomes of brand memory, brand attitudes, and buying intentions. The meta-analysis included 53 experiments involving 8,489 participants. Analyses found that brands advertised in violent media content were remembered less often, evaluated less favorably, and less likely to be purchased than brands advertised in nonviolent, nonsexual media. Brands advertised using sexual ads were evaluated less favorably than brands advertised using nonviolent, nonsexual ads. There were no significant effects of sexual media on memory or buying intentions. There were no significant effects of sexual or violent ads on memory or buying intentions. As intensity of sexual ad content increased, memory, attitudes, and buying intentions decreased. When media content and ad content were congruent (e.g., violent ad in a violent program), memory improved and buying intentions increased. Violence and sex never helped and often hurt ad effectiveness. These results support the evolution and emotional arousal framework. Thus, advertisers should consider the effects of media content, ad content, content intensity, and congruity to design and place more effective ads.

  7. Kinetics and regulation of lactose transport and metabolism in Kluyveromyces lactis JA6.

    PubMed

    Santos, A M; Silveira, W B; Fietto, L G; Brandão, R L; Castro, I M

    2014-07-01

    Kluyveromyces lactis strains are able to assimilate lactose. They have been used industrially to eliminate this sugar from cheese whey and in other industrial products. In this study, we investigated specific features and the kinetic parameters of the lactose transport system in K. lactis JA6. In lactose grown cells, lactose was transported by a system transport with a half-saturation constant (K s) of 1.49 ± 0.38 mM and a maximum velocity (V max) of 0.96 ± 0.12 mmol. (g dry weight)(-1) h(-1) for lactose. The transport system was constitutive and energy-dependent. Results obtained by different approaches showed that the lactose transport system was regulated by glucose at the transcriptional level and by glucose and other sugars at a post-translational level. In K. lactis JA6, galactose metabolization was under glucose control. These findings indicated that the regulation of lactose-galactose regulon in K. lactis was similar to the regulation of galactose regulon in Saccharomyces cerevisiae.

  8. Early dust formation and a massive progenitor for SN 2011ja?

    NASA Astrophysics Data System (ADS)

    Andrews, J. E.; Krafton, Kelsie M.; Clayton, Geoffrey C.; Montiel, E.; Wesson, R.; Sugerman, Ben E. K.; Barlow, M. J.; Matsuura, M.; Drass, H.

    2016-04-01

    SN 2011ja was a bright (I = -18.3) Type II supernova occurring in the nearby edge on spiral galaxy NGC 4945. Flat-topped and multipeaked H α and H β spectral emission lines appear between 64 and 84 d post-explosion, indicating interaction with a disc-like circumstellar medium inclined ˜45° from edge-on. After day 84, an increase in the H- and K-band flux along with heavy attenuation of the red wing of the emission lines are strong indications of early dust formation, likely located in the cool dense shell created between the forward shock of the SN ejecta and the reverse shock created as the ejecta plows into the existing circumstellar material. Radiative transfer modelling reveals both ≈1 × 10-5 M⊙ of pre-existing dust located ˜1016.7 cm away and up to ≈6 × 10-4 M⊙ of newly formed dust. Spectral observations after 1.5 yr reveal the possibility that the fading SN is located within a young (3-6 Myr) massive stellar cluster, which when combined with tentative 56Ni mass estimates of 0.2 M⊙ may indicate a massive (≥25 M⊙) progenitor for SN 2011ja.

  9. Draft genome sequence of Rhodomicrobium udaipurense JA643T with special reference to hopanoid biosynthesis.

    PubMed

    Tushar, L; Sasikala, Ch; Ramana, Ch V

    2014-12-01

    Hopanoids are present in vast amounts as integral components of bacteria and plants with their primary function to strengthen rigidity of the plasma membrane. To establish their roles more precisely, we conducted sequencing of the whole genome of Rhodomicrobium udaipurense JA643(T) isolated from a fresh water stream of Udaipur in Himachal Pradesh, India, by using the Illumina HiSeq pair end chemistry of 2 × 100 bp platform. Determined genome showed a high degree of similarity to the genome of R. vannielii ATCC17100(T) and the 13.7 million reads generated a sequence of 3,649,277 bp possessing 3,611 putative genes. The genomic data were subsequently investigated with respect to genes involved in various features. The machinery required for the degradation of aromatic compounds and resistance to solvents as well as all that required for photosynthesis are present in this organism. Also, through extensive functional annotation, 18 genes involved in the biosynthesis of hopanoids are predicted, namely those responsible for the synthesis of diploptene, diplopterol, adenosylhopane, ribosylhopane, aminobacteriohopanetriol, glycosyl group containing hopanoids and unsaturated hopanoids. The hopanoid biosynthetic pathway was then inferred based on the genes identified and through experimental validation of individual hopanoid molecules. The genome data of R. udaipurense JA643(T) will be useful in understanding the functional features of hopanoids in this bacterium.

  10. Acute oral toxicity of ja-2 solid propellant in sprague-dawley rats. Report for 12 November-19 December 1985

    SciTech Connect

    Brown, L.D.; Justus, J.D.; Wheeler, C.R.; Korte, D.W.

    1989-12-01

    The acute oral toxicity of JA-2 Solid Propellant was determined in male and female Sprague-Dawley rats by using an oral gavage split-dose method. The MLD was 3990.6 + or - 349.7 mg/kg for male rats and 2545.9 + or - 421.1 mg/kg for female rats. JA-2 produced clinical signs that were attributed to its nitrate ester components, diethyleneglycol dinitrate and nitroglycerin. These signs included tremors and twitching, cyanosis, and increases in respiratory rate and depth. Other clinical signs observed were associated with the general malaise of the animals following dosing and included hunched posture, rough coat, reddish stains around the eyes and nose, and perianal staining. Most animals exhibited signs by 4 hours after dosing and either had died or the signs had cleared by 96 hours after dosing. According to the classification scheme of Hodge and Sterner, these results place JA-2 in the slightly toxic class.

  11. Acute oral toxicity of JA-2 solid propellant in icr mice. Report for 17 December 1985-17 January 1986

    SciTech Connect

    Morgan, E.W.; Frost, D.F.; Wheller, C.R.; Korte, D.W.

    1989-12-01

    The acute oral toxicity of JA-2 Solid Propellant was determined in male and female ICR mice by using an oral gavage, split-dose method. The MLD was 3774.6 + or - 150.5 mg/kg for male mice and 3528.8 + or - 133.8 mg/kg for female mice. JA-2 produced component, diethyleneglycol dinitrate and nitroglycerin. These signs included tremors, inactivity, depression of reflexes, loss of equilibrium, opisthotonus, and increased respiratory activity. Other clinical signs observed were associated with the general malaise of the animals following dosing and included perianal staining, hunched posture, squinting, and rough coat. Most animals exhibited signs by 2 hours after dosing and either had died or the signs had cleared within 5 days of dosing. According to the classification scheme of Hodge and Sterner, these results place JA-2 in the slightly toxic class.

  12. Transcriptome sequencing and de novo analysis of cytoplasmic male sterility and maintenance in JA-CMS cotton.

    PubMed

    Yang, Peng; Han, Jinfeng; Huang, Jinling

    2014-01-01

    Cytoplasmic male sterility (CMS) is the failure to produce functional pollen, which is inherited maternally. And it is known that anther development is modulated through complicated interactions between nuclear and mitochondrial genes in sporophytic and gametophytic tissues. However, an unbiased transcriptome sequencing analysis of CMS in cotton is currently lacking in the literature. This study compared differentially expressed (DE) genes of floral buds at the sporogenous cells stage (SS) and microsporocyte stage (MS) (the two most important stages for pollen abortion in JA-CMS) between JA-CMS and its fertile maintainer line JB cotton plants, using the Illumina HiSeq 2000 sequencing platform. A total of 709 (1.8%) DE genes including 293 up-regulated and 416 down-regulated genes were identified in JA-CMS line comparing with its maintainer line at the SS stage, and 644 (1.6%) DE genes with 263 up-regulated and 381 down-regulated genes were detected at the MS stage. By comparing the two stages in the same material, there were 8 up-regulated and 9 down-regulated DE genes in JA-CMS line and 29 up-regulated and 9 down-regulated DE genes in JB maintainer line at the MS stage. Quantitative RT-PCR was used to validate 7 randomly selected DE genes. Bioinformatics analysis revealed that genes involved in reduction-oxidation reactions and alpha-linolenic acid metabolism were down-regulated, while genes pertaining to photosynthesis and flavonoid biosynthesis were up-regulated in JA-CMS floral buds compared with their JB counterparts at the SS and/or MS stages. All these four biological processes play important roles in reactive oxygen species (ROS) homeostasis, which may be an important factor contributing to the sterile trait of JA-CMS. Further experiments are warranted to elucidate molecular mechanisms of these genes that lead to CMS.

  13. South Carolina Guide for Selling.

    ERIC Educational Resources Information Center

    Elliott, Ronald T.

    This curriculum guide provides materials for a secondary course in salesmanship. It contains 6 units that cover 33 competencies. Each competency is presented in a standard format: performance objective, resources, teaching activities, evaluation, and supplementary materials, including an information sheet, exercises, and checklists. Units and…

  14. Selling College Graduates to Employers.

    ERIC Educational Resources Information Center

    Nagy, Daniel R.; Biehl, Kimberly A.

    1980-01-01

    Describes a University of Pittsburgh placement office program to increase placement of graduates with local businesses. To challenge the employment agencies' monopoly on this market, an advertising and direct communications program was launched with continuing good results. (JAC)

  15. Make Locally and Sell Globally

    SciTech Connect

    Majumdar, Arun

    2011-08-15

    The world is in transition and is offering possibly the biggest economic opportunity of the 21st century. The question is: Can we grab it? I believe we can, but we need to be both smart and strategic about it. Let me explain this through a macro-picture and some global trends. First, the long-term trend shows that the global economy (1) will undergo steady growth and, on average, people’s income levels and buying power will rise around the world. This prosperity is the good news. History tells us, however, that consequently in many countries, energy use per person is likely to rise as well (2), especially for developing economies. Access to affordable energy supply as well as their environmental impact poses many risks. Second, the world population is also growing from 6.5 billion people today to almost double by the end of the century, mostly in developing economies. This dual growth of population and the economy suggests that the total world energy demand will very likely increase steadily.

  16. Selling ideas, attitudes, and behaviors.

    PubMed

    Gentile, Douglas A

    2010-04-01

    Advertisers are adept at changing our attitudes and purchasing behaviors, but we rarely notice the effects. This plenary talk at the eighth annual Midwest Rural Agricultural Safety and Health Forum, November 2009, focused on the psychology of advertising and how advertising is designed to work outside of our conscious awareness. Several psychological "tricks" are used to influence us, with the goal being to get us to change our behaviors but to think that it was our idea all along. These tricks include using emotional appeals and persuasion techniques that rely on biases in human problem solving. This power can be used for social marketing, the use of these techniques to promote social well-being, rather than simply for commercial purposes. Understanding how advertising works therefore allows us to use this power to effect positive changes in society.

  17. The Selling of the Subliminal.

    ERIC Educational Resources Information Center

    Levy, Steven

    1984-01-01

    Describes Expandovision, a microcomputer-television connection which enables users to flash brief messages designed to improve their behavior on their television screens. Several incidents where subliminal persuasion has been used on television viewers in the past are recounted, and the effectiveness and dangers of subliminal persuasion are…

  18. How to sell safer sex.

    PubMed

    Overs, C

    1991-09-01

    Social and economic factors determine the extent of the sex industry in societies. Despite AIDS, the sex industry will continue to thrive. Accordingly, health promotion strategies aimed at sex workers and their clients should not stem from the belief that the industry should cease to exist. This paper offers advice in developing and implementing programs to promote safer sex among sex workers. The social context is 1 element to consider in planning successful campaigns. Interventions must be combined with well-planned prevention campaigns aimed at entire populations. The opinions and participation of those involved in the industry should also be sought, while worker discussion and action upon other community issues should not be discouraged. Care should be given to target the numerous and diverse sex worker audiences in addition to other persons related to and involved in the industry. Programs should address the main obstacles to practicing safer sex, and attention should be given to ensure the provision of an adequate and regular supply of cheap or free condoms through varied distribution channels. In the area of service provision, sex workers need easy access to social support and health care services from which they are often excluded. Activities conducted around the world include the marketing of safer sex, distributing printed information on HIV and AIDS to clients, training sex workers to pass designated constructive ideas to others involved in the sex industry, referring sex workers to sex businesses supportive of safer sex practices, and developing street theater and cabaret shows in bars.

  19. Selling Complementary Patents: Experimental Investigation

    SciTech Connect

    Bjornstad, David J; Santore, Rudy; McKee, Michael

    2010-02-01

    Production requiring licensing groups of complementary patents implements a coordination game among patent holders, who can price patents by choosing among combinations of fixed and royalty fees. Summed across patents, these fees become the total producer cost of the package of patents. Royalties, because they function as excise taxes, add to marginal costs, resulting in higher prices and reduced quantities of the downstream product and lower payoffs to the patent holders. Using fixed fees eliminates this inefficiency but yields a more complex coordination game in which there are multiple equilibria, which are very fragile in that small mistakes can lead the downstream firm to not license the technology, resulting in inefficient outcomes. We report on a laboratory market investigation of the efficiency effects of coordinated pricing of patents in a patent pool. We find that pool-like pricing agreements can yield fewer coordination failures in the pricing of complementary patents.

  20. Best-Selling Children's Books.

    ERIC Educational Resources Information Center

    Kujoth, Jean Spealman

    This book reports the results of a survey by the author to determine what children's books have been bought for, and read by, the most children. It identifies and describes 958 children's grade books now in print that, according to U.S. publishers, have sold 100,000 copies or more since their publication. These best-sellers are listed by author,…

  1. The Selling of Student Loans

    ERIC Educational Resources Information Center

    Field, Kelly

    2007-01-01

    Twenty years ago, a U.S. Senate aide walked into a bank in Maine and saw a sign that read "Take out a student loan today, get a toaster." The aide, concerned that students would be tempted to take out loans they did not need, drafted legislation barring lenders from offering "inducements" to borrow. That language was added to the Higher Education…

  2. Banana fruit VQ motif-containing protein5 represses cold-responsive transcription factor MaWRKY26 involved in the regulation of JA biosynthetic genes

    PubMed Central

    Ye, Yu-Jie; Xiao, Yun-Yi; Han, Yan-Chao; Shan, Wei; Fan, Zhong-Qi; Xu, Qun-Gang; Kuang, Jian-Fei; Lu, Wang-Jin; Lakshmanan, Prakash; Chen, Jian-Ye

    2016-01-01

    Most harvested fruits and vegetables are stored at low temperature but many of them are highly sensitive to chilling injury. Jasmonic acid (JA), a plant hormone associated with various stress responses, is known to reduce chilling injury in fruits. However, little is known about the transcriptional regulation of JA biosynthesis in relation to cold response of fruits. Here, we show the involvement of a Group I WRKY transcription factor (TF) from banana fruit, MaWRKY26, in regulating JA biosynthesis. MaWRKY26 was found to be nuclear-localized with transcriptional activation property. MaWRKY26 was induced by cold stress or by methyl jasmonate (MeJA), which enhances cold tolerance in banana fruit. More importantly, MaWRKY26 transactivated JA biosynthetic genes MaLOX2, MaAOS3 and MaOPR3 via binding to their promoters. Further, MaWRKY26 physically interacted with a VQ motif-containing protein MaVQ5, and the interaction attenuated MaWRKY26-induced transactivation of JA biosynthetic genes. These results strongly suggest that MaVQ5 might act as a repressor of MaWRKY26 in activating JA biosynthesis. Taken together, our findings provide new insights into the transcriptional regulation of JA biosynthesis in response to cold stress and a better understanding of the molecular aspects of chilling injury in banana fruit. PMID:27004441

  3. HiJaK: the high-resolution J, H and K spectrometer

    NASA Astrophysics Data System (ADS)

    Muirhead, Philip S.; Hall, Zachary J.; Veyette, Mark J.

    2014-08-01

    We present the science drivers, design requirements and a preliminary design for a high-resolution, broad- bandwidth, slit-fed cross-dispersed near-infrared spectrometer for 5-meter-class telescopes. Our concept, called the High-Resolution J, H and K Spectrometer, or HiJaK, utilizes an R6 echelle in a white-pupil design to achieve high resolution in a compact configuration with a 2048 x 2048 pixel infrared detector. We present a preliminary ray-traced optical design matched to the new 4.3-meter Discovery Channel Telescope in Happy Jack, Arizona. We also discuss mechanical and cryogenic options to house our optical design.

  4. High Temperature Induces Expression of Tobacco Transcription Factor NtMYC2a to Regulate Nicotine and JA Biosynthesis

    PubMed Central

    Yang, Liming; Li, Junying; Ji, Jianhui; Li, Ping; Yu, Liangliang; Abd_Allah, Elsayed F.; Luo, Yuming; Hu, Liwei; Hu, Xiangyang

    2016-01-01

    Environmental stress elevates the level of jasmonic acid (JA) and activates the biosynthesis of nicotine and related pyridine alkaloids in tobacco (Nicotiana tabacum L.) by up-regulating the expression of putrescine N-methyltransferase 1 (NtPMT1), which encodes a putrescine N-methyl transferase that catalyzes nicotine formation. The JA signal suppressor JASMONATE ZIM DOMAIN 1 (NtJAZ1) and its target protein, NtMYC2a, also regulate nicotine biosynthesis; however, how these proteins interact to regulate abiotic-induced nicotine biosynthesis is poorly understood. In this study, we found that high-temperature (HT) treatment activated transcription of NtMYC2a, which subsequently stimulated the transcription of genes associated with JA biosynthesis, including Lipoxygenase (LOX), Allene oxide synthase (AOS), Allene oxide cyclase (AOC), and 12-oxophytodienodate reductase (OPR). Overexpression of NtMYC2a increased nicotine biosynthesis by enhancing its binding to the promoter of NtPMT1. Overexpression of either NtJAZ1 or proteasome-resistant NtJAZ1ΔC suppressed nicotine production under normal conditions, but overexpression only of the former resulted in low levels of nicotine under HT treatment. These data suggest that HT induces NtMYC2a accumulation through increased transcription to activate nicotine synthesis; meanwhile, HT-induced NtMYC2a can activate JA synthesis to promote additional NtMYC2a activity by degrading NtJAZ1 at the post-transcriptional level. PMID:27833561

  5. Overexpression of OsMYC2 Results in the Up-Regulation of Early JA-Rresponsive Genes and Bacterial Blight Resistance in Rice.

    PubMed

    Uji, Yuya; Taniguchi, Shiduku; Tamaoki, Daisuke; Shishido, Hodaka; Akimitsu, Kazuya; Gomi, Kenji

    2016-09-01

    JASMONATE ZIM-domain (JAZ) proteins act as transcriptional repressors of jasmonic acid (JA) responses and play a crucial role in the regulation of host immunity in plants. Here, we report that OsMYC2, a JAZ-interacting transcription factor in rice (Oryza sativa L.), plays an important role in the resistance response against rice bacterial blight, which is one of the most serious diseases in rice, caused by Xanthomonas oryzae pv. oryzae (Xoo). The results showed that OsMYC2 interacted with some OsJAZ proteins in a JAZ-interacting domain (JID)-dependent manner. The up-regulation of OsMYC2 in response to JA was regulated by OsJAZ8. Transgenic rice plants overexpressing OsMYC2 exhibited a JA-hypersensitive phenotype and were more resistant to Xoo. A large-scale microarray analysis revealed that OsMYC2 up-regulated OsJAZ10 as well as many other defense-related genes. OsMYC2 selectively bound to the G-box-like motif of the OsJAZ10 promoter in vivo and regulated the expression of early JA-responsive genes, but not of late JA-responsive genes. The nuclear localization of OsMYC2 depended on a nuclear localization signal within JID. Overall, we conclude that OsMYC2 acts as a positive regulator of early JA signals in the JA-induced resistance against Xoo in rice.

  6. bHLH003, bHLH013 and bHLH017 Are New Targets of JAZ Repressors Negatively Regulating JA Responses

    PubMed Central

    Fonseca, Sandra; Fernández-Calvo, Patricia; Fernández, Guillermo M.; Díez-Díaz, Monica; Gimenez-Ibanez, Selena; López-Vidriero, Irene; Godoy, Marta; Fernández-Barbero, Gemma; Van Leene, Jelle; De Jaeger, Geert; Franco-Zorrilla, José Manuel; Solano, Roberto

    2014-01-01

    Cell reprogramming in response to jasmonates requires a tight control of transcription that is achieved by the activity of JA-related transcription factors (TFs). Among them, MYC2, MYC3 and MYC4 have been described as activators of JA responses. Here we characterized the function of bHLH003, bHLH013 and bHLH017 that conform a phylogenetic clade closely related to MYC2, MYC3 and MYC4. We found that these bHLHs form homo- and heterodimers and also interact with JAZ repressors in vitro and in vivo. Phenotypic analysis of JA-regulated processes, including root and rosette growth, anthocyanin accumulation, chlorophyll loss and resistance to Pseudomonas syringae, on mutants and overexpression lines, suggested that these bHLHs are repressors of JA responses. bHLH003, bHLH013 and bHLH017 are mainly nuclear proteins and bind DNA with similar specificity to that of MYC2, MYC3 and MYC4, but lack a conserved activation domain, suggesting that repression is achieved by competition for the same cis-regulatory elements. Moreover, expression of bHLH017 is induced by JA and depends on MYC2, suggesting a negative feed-back regulation of the activity of positive JA-related TFs. Our results suggest that the competition between positive and negative TFs determines the output of JA-dependent transcriptional activation. PMID:24465948

  7. JA, a new type of polyunsaturated fatty acid isolated from Juglans mandshurica Maxim, limits the survival and induces apoptosis of heptocarcinoma cells.

    PubMed

    Gao, Xiu-Li; Lin, Hua; Zhao, Wei; Hou, Ya-Qin; Bao, Yong-Li; Song, Zhen-Bo; Sun, Lu-Guo; Tian, Shang-Yi; Liu, Biao; Li, Yu-Xin

    2016-03-01

    Juglans mandshurica Maxim (Juglandaceae) is a famous folk medicine for cancer treatment and some natural compounds isolated from it have been studied extensively. Previously we isolated a type of ω-9 polyunsaturated fatty acid (JA) from the bark of J. mandshurica, however little is known about its activity and the underlying mechanisms. In this study, we studied anti-tumor activity of JA on several human cancer cell lines. Results showed that JA is cytotoxic to HepG2, MDA-MB-231, SGC-7901, A549 and Huh7 cells at a concentration exerting minimal toxic effects on L02 cells. The selective toxicity of JA was better than other classical anti-cancer drugs. Further investigation indicated that JA could induce cell apoptosis, characterized by chromatin condensation, DNA fragmentation and activation of the apoptosis-associated proteins such as Caspase-3 and PARP-1. Moreover, we investigated the cellular apoptosis pathway involved in the apoptosis process in HepG2 cells. We found that proteins involved in mitochondrion (cleaved-Caspase-9, Apaf-1, HtrA2/Omi, Bax, and Mitochondrial Bax) and endocytoplasmic reticulum (XBP-1s, GRP78, cleaved-Caspase-7 and cleaved-Caspase-12) apoptotic pathways were up-regulated when cells were treated by JA. In addition, a morphological change in the mitochondrion was detected. Furthermore, we found that JA could inhibit DNA synthesis and induce G2/M cell cycle arrest. The expression of G2-to-M transition related proteins, such as CyclinB1 and phosphorylated-CDK1, were reduced. In contrast, the G2-to-M inhibitor p21 was increased in JA-treated cells. Overall, our results suggest that JA can induce mitochondrion- and endocytoplasmic reticulum-mediated apoptosis, and G2/M phase arrest in HepG2 cells, making it a promising therapeutic agent against hepatoma.

  8. Seed germination ecology of feather lovegrass [Eragrostis tenella (L.) Beauv. Ex Roemer & J.A. Schultes].

    PubMed

    Chauhan, Bhagirath S

    2013-01-01

    Feather lovegrass [Eragrostis tenella (L.) Beauv. Ex Roemer & J.A. Schultes] is a C4 grass weed that has the ability to grow in both lowland and upland conditions. Experiments were conducted in the laboratory and screenhouse to evaluate the effect of environmental factors on germination, emergence, and growth of this weed species. Germination in the light/dark regime was higher at alternating day/night temperatures of 30/20 °C (98%) than at 35/25 °C (83%) or 25/15 °C (62%). Germination was completely inhibited by darkness. The osmotic potential and sodium chloride concentrations required for 50% inhibition of maximum germination were -0.7 MPa and 76 mM, respectively. The highest seedling emergence (69%) was observed from the seeds sown on the soil surface and no seedlings emerged from seeds buried at depths of 0.5 cm or more. The use of residue as mulches significantly reduced the emergence and biomass of feather lovegrass seedlings. A residue amount of 0.5 t ha(-1) was needed to suppress 50% of the maximum seedlings. Because germination was strongly stimulated by light and seedling emergence was the highest for the seeds sown on the soil surface, feather lovegrass is likely to become a problematic weed in zero-till systems. The knowledge gained from this study could help in developing effective and sustainable weed management strategies.

  9. High-Rate Mechanical Properties of JA2 Propellant at Temperatures from -50 to 80 deg C

    DTIC Science & Technology

    2015-07-01

    Joyce E Newberry 5d. PROJECT NUMBER 622618.H8000 5e. TASK NUMBER 5f. WORK UNIT NUMBER 7. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) US...Army Research Laboratory ATTN: RDRL-WML-D Aberdeen Proving Ground, MD 21005-5069 8. PERFORMING ORGANIZATION REPORT NUMBER ARL-MR-0894 9...AVAILABILITY STATEMENT Approved for public release; distribution is unlimited. 13. SUPPLEMENTARY NOTES 14. ABSTRACT JA2, a viscoelastic polymeric

  10. 40 CFR Table 1 to Subpart Ja of... - Molar Exhaust Volumes and Molar Heat Content of Fuel Gas Constituents

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Content of Fuel Gas Constituents 1 Table 1 to Subpart Ja of Part 60 Protection of Environment... Exhaust Volumes and Molar Heat Content of Fuel Gas Constituents Constituent MEVa dscf/mol MHCb Btu/mol Methane (CH4) 7.29 842 Ethane (C2H6) 12.96 1,475 Hydrogen (H2) 1.61 269 Ethene (C2H4) 11.34 1,335...

  11. Visualization and Measurement of the Burning Surface of Wire-Embedded Energetic Materials, Part 1: JA2 and Pentolite

    DTIC Science & Technology

    2014-06-01

    figure 4). The apparatus includes a windowed chamber capable of being pressurized up to 10 MPa. It also includes a ballast tank that adds considerably... pressures were acquired, and they can be employed to validate a state-of-the-art CFD model that ARL is developing to accelerate the development of...rates at pressure near 5.18 MPa. ..............................................................12 Figure 11. JA2 burning rates at 6.90 MPa

  12. Diverting the flux of the JA pathway in Nicotiana attenuata compromises the plant's defense metabolism and fitness in nature and glasshouse.

    PubMed

    Stitz, Michael; Baldwin, Ian T; Gaquerel, Emmanuel

    2011-01-01

    A plant's inducible defenses against herbivores as well as certain developmental processes are known to be controlled by the jasmonic acid (JA) pathway. We have previously shown that ectopically expressing Arabidopsis thaliana JA O-methyltransferase in Nicotiana attenuata (35S-jmt) strongly reduces the herbivory-elicited jasmonate bursts by acting as metabolic sink that redirects free JA towards methylation; here we examine the consequences of this metabolic sink on N. attenuata's secondary metabolism and performance in nature. In the glasshouse, 35S-jmt plants produced fewer seed capsules due to shorter floral styles, which could be restored to wild type (WT) levels after hand-pollination, and were more susceptible to Manduca sexta larvae attack. When transplanted into the Great Basin Desert in Utah, 35S-jmt plants grew as well as WT empty vector, but were highly attacked by native herbivores of different feeding guilds: leaf chewers, miners, and single cell feeders. This greater susceptibility was strongly associated with reduced emissions of volatile organic compounds (hexenylesters, monoterpenes and sesquiterpenes) and profound alterations in the production of direct defenses (trypsin proteinase inhibitors [TPI], nicotine, diterpene glycosides [DTGs] and phenylpropanoid-polyamine conjugates) as revealed by a combination of targeted and metabolomics analyses of field collected samples. Complementation experiments with JA-Ile, whose formation is outcompeted in 35S-jmt plants by the methylation reaction, restored the local TPI activation to WT levels and partially complemented nicotine and DTG levels in elicited but not systemic leaves. These findings demonstrate that MeJA, the major JA metabolite in 35S-jmt plants, is not an active signal in defense activation and highlights the value of creating JA sinks to disrupt JA signaling, without interrupting the complete octadecanoid pathway, in order to investigate the regulation of plants' defense metabolism in nature.

  13. A bHLH-Type Transcription Factor, ABA-INDUCIBLE BHLH-TYPE TRANSCRIPTION FACTOR/JA-ASSOCIATED MYC2-LIKE1, Acts as a Repressor to Negatively Regulate Jasmonate Signaling in Arabidopsis[C][W

    PubMed Central

    Nakata, Masaru; Mitsuda, Nobutaka; Herde, Marco; Koo, Abraham J.K.; Moreno, Javier E.; Suzuki, Kaoru; Howe, Gregg A.; Ohme-Takagi, Masaru

    2013-01-01

    Jasmonates (JAs) are plant hormones that regulate the balance between plant growth and responses to biotic and abiotic stresses. Although recent studies have uncovered the mechanisms for JA-induced responses in Arabidopsis thaliana, the mechanisms by which plants attenuate the JA-induced responses remain elusive. Here, we report that a basic helix-loop-helix–type transcription factor, ABA-INDUCIBLE BHLH-TYPE TRANSCRIPTION FACTOR/JA-ASSOCIATED MYC2-LIKE1 (JAM1), acts as a transcriptional repressor and negatively regulates JA signaling. Gain-of-function transgenic plants expressing the chimeric repressor for JAM1 exhibited substantial reduction of JA responses, including JA-induced inhibition of root growth, accumulation of anthocyanin, and male fertility. These plants were also compromised in resistance to attack by the insect herbivore Spodoptera exigua. Conversely, jam1 loss-of-function mutants showed enhanced JA responsiveness, including increased resistance to insect attack. JAM1 and MYC2 competitively bind to the target sequence of MYC2, which likely provides the mechanism for negative regulation of JA signaling and suppression of MYC2 functions by JAM1. These results indicate that JAM1 negatively regulates JA signaling, thereby playing a pivotal role in fine-tuning of JA-mediated stress responses and plant growth. PMID:23673982

  14. OsMYC2, an essential factor for JA-inductive sakuranetin production in rice, interacts with MYC2-like proteins that enhance its transactivation ability

    PubMed Central

    Ogawa, Satoshi; Miyamoto, Koji; Nemoto, Keiichirou; Sawasaki, Tatsuya; Yamane, Hisakazu; Nojiri, Hideaki; Okada, Kazunori

    2017-01-01

    Biosynthesis of sakuranetin, a flavonoid anti-fungal phytoalexin that occurs in rice, is highly dependent on jasmonic acid (JA) signalling and induced by a variety of environmental stimuli. We previously identified OsNOMT, which encodes naringenin 7-O-methyltransferase (NOMT); NOMT is a key enzyme for sakuranetin production. Although OsNOMT expression is induced by JA treatment, the regulation mechanism that activates the biosynthetic pathway of sakuranetin has not yet been elucidated. In this study, we show that JA-inducible basic helix-loop-helix transcriptional factor OsMYC2 drastically enhances the activity of the OsNOMT promoter and is essential for JA-inducible sakuranetin production. In addition, we identified 2 collaborators of OsMYC2, OsMYC2-like protein 1 and 2 (OsMYL1 and OsMYL2) that further activated the OsNOMT promoter in synergy with OsMYC2. Physical interaction of OsMYC2 with OsMYL1 and OsMYL2 further supported the idea that these interactions lead to the enhancement of the transactivation activity of OsMYC2. Our results indicate that JA signalling via OsMYC2 is reinforced by OsMYL1 and OsMYL2, resulting in the inductive production of sakuranetin during defence responses in rice. PMID:28067270

  15. JaTS: a fully portable seismic tomography software based on Fresnel wavepaths and a probabilistic reconstruction approach

    NASA Astrophysics Data System (ADS)

    Grandjean, Gilles; Sage, Sandrine

    2004-11-01

    JaTS, a Java 2D seismic tomography software, is presented. It implements original algorithms achieving optimal accuracy with reasonable computing costs. A second-order Fast Marching Method (FMM) is used for solving the eikonal equation, therefore enabling a fast and robust computation of seismic traveltimes between sources and receivers. The wavepaths are materialized by Fresnel volumes rather than by conventional rays. This approach accounts for complex velocity models and has the advantage of considering the effects of the wave frequency in the velocity model resolution. The model is computed by a Simultaneous Iterative Reconstruction Technique (SIRT) which has been reformulated to integrate Fresnel wavepaths by using a probabilistic approach. In addition, various utilities are implemented, such as a tapering filter, used to decrease artifact effects occurring in the vicinity of the sources and receivers. The software also offers the possibility of reconstructing the velocity field on a grid larger than the one used for the wave propagation computation. This contributes to stabilize the estimated values. All of the seismic processing tools have been integrated with a user-friendly graphical interface. JaTS represents a tightly integrated tool suite that supports the entire process of importing the SG2 field records, first-break picking, forward modeling and velocity-field computing across multiple platforms.

  16. A pilot study on post-evisceration contamination of broiler carcasses and ready-to-sell livers and intestines (mala) with Campylobacter jejuni and Campylobacter coli in a high-throughput South African poultry abattoir.

    PubMed

    Bartkowiak-Higgo, A J; Veary, C M; Venter, E H; Bosman, A M

    2006-09-01

    To assess post-evisceration contamination of broiler carcasses, 300 samples were randomly selected during routine slaughter in the winter of 2004. The samples originated from 50 chicken carcasses, taken directly after evisceration, as well as 25 samples from ready-to-sell packages of fresh intestines (mala) and livers. The samples were taken in batches over a period of 4 weeks to allow randomised sampling from different farms of origin. Conventional culture-based detection methods of Campylobacter spp. usually need 4-6 days to produce a result. The polymerase chain reaction (PCR) used for this study took less than 32 hours. The average contamination rates with Campylobacter in both the skin and liver samples were 24%, and 28% for intestines. Chicken and chicken products, especially livers and intestines, form an integral part of the traditional diet of many Black South Africans, as they are cheap and readily available in bulk and un-chilled for direct distribution, mainly through street vending and other informal retail outlets. This sudy showed that Campylobacter spp. are prevalent in poultry in South Africa. The handling of poultry meat and products contaminated with this organism in households and the potential for cross-contamination of other foods presents a high risk of infection to consumers in South Africa. The study also emphasised the need for further research in this field.

  17. Integrated Performance of Next Generation High Data Rate Receiver and AR4JA LDPC Codec for Space Communications

    NASA Technical Reports Server (NTRS)

    Cheng, Michael K.; Lyubarev, Mark; Nakashima, Michael A.; Andrews, Kenneth S.; Lee, Dennis

    2008-01-01

    Low-density parity-check (LDPC) codes are the state-of-the-art in forward error correction (FEC) technology that exhibits capacity approaching performance. The Jet Propulsion Laboratory (JPL) has designed a family of LDPC codes that are similar in structure and therefore, leads to a single decoder implementation. The Accumulate-Repeat-by-4-Jagged- Accumulate (AR4JA) code design offers a family of codes with rates 1/2, 2/3, 4/5 and lengths 1024, 4096, 16384 information bits. Performance is less than one dB from capacity for all combinations.Integrating a stand-alone LDPC decoder with a commercial-off-the-shelf (COTS) receiver faces additional challenges than building a single receiver-decoder unit from scratch. In this work, we outline the issues and show that these additional challenges can be over-come by simple solutions. To demonstrate that an LDPC decoder can be made to work seamlessly with a COTS receiver, we interface an AR4JA LDPC decoder developed on a field-programmable gate array (FPGA) with a modern high data rate receiver and mea- sure the combined receiver-decoder performance. Through optimizations that include an improved frame synchronizer and different soft-symbol scaling algorithms, we show that a combined implementation loss of less than one dB is possible and therefore, most of the coding gain evidence in theory can also be obtained in practice. Our techniques can benefit any modem that utilizes an advanced FEC code.

  18. The MeJA-inducible copper amine oxidase AtAO1 is expressed in xylem tissue and guard cells.

    PubMed

    Ghuge, Sandip A; Carucci, Andrea; Rodrigues-Pousada, Renato A; Tisi, Alessandra; Franchi, Stefano; Tavladoraki, Paraskevi; Angelini, Riccardo; Cona, Alessandra

    2015-01-01

    Copper amine oxidases oxidize the polyamine putrescine to 4-aminobutanal with the production of the plant signal molecule hydrogen peroxide (H2O2) and ammonia. The Arabidopsis (Arabidopsis thaliana) gene At4g14940 (AtAO1, previously referred to as ATAO1) encodes an apoplastic copper amine oxidase expressed in lateral root cap cells and developing xylem, especially in root protoxylem and metaxylem precursors. In our recent study, we demonstrated that AtAO1 expression is strongly induced in the root vascular tissues by the wound-signal hormone methyl jasmonate (MeJA). Furthermore, we also demonstrated that the H2O2 derived by the AtAO1-driven oxidation of putrescine, mediates the MeJA-induced early protoxylem differentiation in Arabidopsis roots. H2O2 may contribute to protoxylem differentiation by signaling developmental cell death and by acting as co-substrate in peroxidase-mediated cell wall stiffening and lignin polymerization. Here, by the means of AtAO1 promoter::green fluorescent protein-β-glucuronidase (AtAO1::GFP-GUS) fusion analysis, we show that a strong AtAO1 gene expression occurs also in guard cells of leaves and flowers. The high expression levels of AtAO1 in tissues or cell types regulating water supply and water loss may suggest a role of the encoded protein in water balance homeostasis, by modulating coordinated adjustments in anatomical and functional features of xylem tissue and guard cells during acclimation to adverse environmental conditions.

  19. Structural basis of jasmonate-amido synthetase FIN219 in complex with glutathione S-transferase FIP1 during the JA signal regulation

    PubMed Central

    Chen, Chun-Yen; Ho, Sih-Syun; Kuo, Tzu-Yen; Cheng, Yi-Sheng

    2017-01-01

    Far-red (FR) light-coupled jasmonate (JA) signaling is necessary for plant defense and development. FR insensitive 219 (FIN219) is a member of the Gretchen Hagen 3 (GH3) family of proteins in Arabidopsis and belongs to the adenylate-forming family of enzymes. It directly controls biosynthesis of jasmonoyl-isoleucine in JA-mediated defense responses and interacts with FIN219-interacting protein 1 (FIP1) under FR light conditions. FIN219 and FIP1 are involved in FR light signaling and are regulators of the interplay between light and JA signaling. However, how their interactions affect plant physiological functions remains unclear. Here, we demonstrate the crystal structures of FIN219–FIP1 while binding with substrates at atomic resolution. Our results show an unexpected FIN219 conformation and demonstrate various differences between this protein and other members of the GH3 family. We show that the rotated C-terminal domain of FIN219 alters ATP binding and the core structure of the active site. We further demonstrate that this unique FIN219–FIP1 structure is crucial for increasing FIN219 activity and determines the priority of substrate binding. We suggest that the increased FIN219 activity resulting from the complex form, a conformation for domain switching, allows FIN219 to switch to its high-affinity mode and thereby enhances JA signaling under continuous FR light conditions. PMID:28223489

  20. MdMYB9 and MdMYB11 are involved in the regulation of the JA-induced biosynthesis of anthocyanin and proanthocyanidin in apples.

    PubMed

    An, Xiu-Hong; Tian, Yi; Chen, Ke-Qin; Liu, Xiao-Juan; Liu, Dan-Dan; Xie, Xing-Bin; Cheng, Cun-Gang; Cong, Pei-Hua; Hao, Yu-Jin

    2015-04-01

    Anthocyanin and proanthocyanidin (PA) are important secondary metabolites and beneficial to human health. Their biosynthesis is induced by jasmonate (JA) treatment and regulated by MYB transcription factors (TFs). However, which and how MYB TFs regulate this process is largely unknown in apple. In this study, MdMYB9 and MdMYB11 which were induced by methyl jasmonate (MeJA) were functionally characterized. Overexpression of MdMYB9 or MdMYB11 promoted not only anthocyanin but also PA accumulation in apple calluses, and the accumulation was further enhanced by MeJA. Subsequently, yeast two-hybrid, pull-down and bimolecular fluorescence complementation assays showed that both MYB proteins interact with MdbHLH3. Moreover, Jasmonate ZIM-domain (MdJAZ) proteins interact with MdbHLH3. Furthermore, chromatin immunoprecipitation-quantitative PCR and yeast one-hybrid assays demonstrated that both MdMYB9 and MdMYB11 bind to the promoters of ANS, ANR and LAR, whereas MdbHLH3 is recruited to the promoters of MdMYB9 and MdMYB11 and regulates their transcription. In addition, transient expression assays indicated that overexpression of MdJAZ2 inhibits the recruitment of MdbHLH3 to the promoters of MdMYB9 and MdMYB11. Our findings provide new insight into the mechanism of how MeJA regulates anthocyanin and PA accumulation in apple.

  1. Genome Analysis of the Biotechnologically Relevant Acidophilic Iron Oxidising Strain JA12 Indicates Phylogenetic and Metabolic Diversity within the Novel Genus “Ferrovum”

    PubMed Central

    Ullrich, Sophie R.; Poehlein, Anja; Tischler, Judith S.; González, Carolina; Ossandon, Francisco J.; Daniel, Rolf; Holmes, David S.; Schlömann, Michael; Mühling, Martin

    2016-01-01

    Background Members of the genus “Ferrovum” are ubiquitously distributed in acid mine drainage (AMD) waters which are characterised by their high metal and sulfate loads. So far isolation and microbiological characterisation have only been successful for the designated type strain “Ferrovum myxofaciens” P3G. Thus, knowledge about physiological characteristics and the phylogeny of the genus “Ferrovum” is extremely scarce. Objective In order to access the wider genetic pool of the genus “Ferrovum” we sequenced the genome of a “Ferrovum”-containing mixed culture and successfully assembled the almost complete genome sequence of the novel “Ferrovum” strain JA12. Phylogeny and Lifestyle The genome-based phylogenetic analysis indicates that strain JA12 and the type strain represent two distinct “Ferrovum” species. “Ferrovum” strain JA12 is characterised by an unusually small genome in comparison to the type strain and other iron oxidising bacteria. The prediction of nutrient assimilation pathways suggests that “Ferrovum” strain JA12 maintains a chemolithoautotrophic lifestyle utilising carbon dioxide and bicarbonate, ammonium and urea, sulfate, phosphate and ferrous iron as carbon, nitrogen, sulfur, phosphorous and energy sources, respectively. Unique Metabolic Features The potential utilisation of urea by “Ferrovum” strain JA12 is moreover remarkable since it may furthermore represent a strategy among extreme acidophiles to cope with the acidic environment. Unlike other acidophilic chemolithoautotrophs “Ferrovum” strain JA12 exhibits a complete tricarboxylic acid cycle, a metabolic feature shared with the closer related neutrophilic iron oxidisers among the Betaproteobacteria including Sideroxydans lithotrophicus and Thiobacillus denitrificans. Furthermore, the absence of characteristic redox proteins involved in iron oxidation in the well-studied acidophiles Acidithiobacillus ferrooxidans (rusticyanin) and Acidithiobacillus

  2. Selling wind: Lessons in green niche marketing

    NASA Astrophysics Data System (ADS)

    Worden, Gregory Edward

    Concern about global warming, energy independence, and threats to oil supply have increased attention on wind and other forms of renewable energy. Yet after more than twenty years, the wind industry remains dependent on government interventions. This research examined the potential of renewable energy credits (RECs) to help wind energy become profitable. Messages used to promote wind and solar energy RECs were compared with those for sustainable building materials. Findings confirm a still immature approach to marketing and sales. None of those interviewed either recognized the value of or had taken action to ensure customer retention nor recognized the role socially conscious and active consumers might play in promoting and helping develop the industry. Recommended actions include continuing research on effective marketing strategies and development of a coordinated industry message.

  3. Nanoscale optimization of quantum dot solar sells

    NASA Astrophysics Data System (ADS)

    Li, Yanshu; Sergeev, Andrei; Vagidov, Nizami; Mitin, Vladimir; Sablon, Kimberly; State Univ of NY-Buffalo Team; Army Research Laboratory Team

    2015-03-01

    Quantum dots (QDs) offer possibilities for nanoscale control of photoelectron processes via engineering the band structure and potential profile. Nanoscale potential profile (potential barriers) and nanoscale band engineering (AlGaAs atomically thin barriers) effectively suppress the photoelectron capture to QDs. QDs also increase conversion efficiency of the above-bandgap photons due to extraction of electrons from QDs via Coulomb interaction with hot electrons that excited by high-energy photons. To study the effects of the band structure engineering and nanoscale potential barriers on the photovoltaic performance we fabricated 3- μm base GaAs devices with various InAs quantum dot media and selective doping. All quantum dot devices show improvement in conversion efficiency compared with the reference cell. Quantum efficiency measurements allow us to associate the spectral characteristics of photoresponse enhancement with nanoscale structure of QD media. The dark current analysis provides valuable information about recombination in QD solar cells. The two-diode model well fit the scope of data and recovers the measured open circuit voltage.

  4. Simplicity sells: Making smoking cessation easier.

    PubMed

    Bonniot Saucedo, Catherine; Schroeder, Steven A

    2010-03-01

    Toll-free telephone quitlines are successful alternatives to direct clinician contact. In 2004, the U.S. Department of Health and Human Services created a national quitline number, 1-800-QUIT-NOW. This enabled states without quitlines to establish them, giving free access to cessation services to every smoker in the U.S. It also created a new mechanism for national quitline marketing, employing simplified and streamlined approaches.

  5. WHAT DOES IT TAKE TO SELL FEED.

    ERIC Educational Resources Information Center

    ALBRACHT, JAMES J.

    TO DETERMINE THE VOCATIONAL COMPETENCIES NECESSARY FOR THE PERFORMANCE OF NINE ESSENTIAL SALES ACTIVITIES IN THE FEED INDUSTRY, A JURY OF 24 FEED DEALERS, SALES TRAINING DIRECTORS, AGRICULTURAL EDUCATION RESEARCHERS, AND BUSINESS EDUCATION RESEARCHERS MADE "YES" AND "NO" DETERMINATIONS FOR 40 COMPETENCIES. THE NUMBER OF COMPETENCIES CONSIDERED…

  6. The Selling of Cable Television 1972.

    ERIC Educational Resources Information Center

    National Cable Television Association, Inc., Washington, DC.

    The 1972 Cable Television Marketing Workshop reviewed in depth a wide variety of marketing and public relations techniques as they pertain to cable television. The workshop was attended by representatives of commercial television systems throughout the United States; it was intended to disseminate the sales and marketing experience of those…

  7. Analysis of Army Recruiter Selling Techniques.

    DTIC Science & Technology

    1991-04-01

    that have been put forward in recent years to explain consumer choices and consumer behavior . Each of these will be explored in the following...Allyn and Bacon. Mittelstaedt, R. A. (1971). Criteria for a theory of consumer behavior . In Robert J. Holloway, Robert A. Mittelstaedt, and M...Verkatesan (Eds.), Consumer Behavior (pp. 8-13). Boston: Houghton-Mifflin. 𔃿 Mittelstaedt, R. A. and Madden, C. S. (1980). Lay psychology, marketing

  8. How to sell services more profitably.

    PubMed

    Reinartz, Werner; Ulaga, Wolfgang

    2008-05-01

    When products become commodities, manufacturing companies may seek to differentiate themselves with value-added services--a potentially profitable strategy. Unfortunately, companies often stumble in the effort. Reinartz and Ulaga conducted in-depth studies of 18 leading companies in a broad variety of product markets to learn what distinguished the successes from the rest. They discovered four steps to developing a profitable services capability. RECOGNIZE THAT YOU ALREADY HAVE A SERVICE COMPANY: You can identify and charge for simple services--as Merck did when it stopped quietly absorbing shipping costs. Switching services from free to fee clarifies their value for managers as well as for customers. INDUSTRIALIZE THE BACK OFFICE: To prevent delivery costs from eating up service-offering margins, build flexible service platforms, closely monitor process costs, and exploit new technologies that enable process innovations. The Swedish bearings manufacturer SKF provided off-site access to an online monitoring tool that could warn of potential failure in customers' machines. CREATE A SERVICE-SAVVY SALES FORCE: Services require longer sales cycles and, often, decisions from high up in a customer's hierarchy; what's more, product salespeople may be inimical to change. Schneider-Electric did a major overhaul of its sales organization and trained its people to switch from cost-plus pricing to value-based pricing. FOCUS ON CUSTOMERS' PROCESSES AND THE OPPORTUNITIES THEY AFFORD FOR NEW SERVICE OFFERINGS: You may need to acquire new capabilities to take advantage of those opportunities: The industrial coatings specialist PPG had to learn how painting robots function after it offered to take over Fiat's Torino paint shop. Services can both lock in customers and help acquire new accounts. They should be developed with care and attention.

  9. Avoiding potential problems when selling accounts receivable.

    PubMed

    Ayers, D H; Kincaid, T J

    1996-05-01

    Accounts receivable financing is a potential tool for managing a provider organization's working capital needs. But before entering into a financing agreement, organizations need to consider and take steps to avoid serious problems that can arise from participation in an accounts receivable financing program. For example, the purchaser may cease purchasing the receivables, leaving the organization without funding needed for operations. Or, the financing program may be inordinately complex and unnecessarily costly to the organization. Sometimes the organization itself may fail to comply with the terms of the agreement under which the accounts receivable were sold, thus necessitating that restitution be made to the purchaser or provoking charges of fraud. These potential problems should be addressed as early as possible--before an organization enters into an accounts receivable financing program--in order to minimize time, effort, and expanse and maximize the benefits of the financing agreement.

  10. Chinese Summer Schools Sell Quick Credits

    ERIC Educational Resources Information Center

    McMurtrie, Beth; Farrar, Lara

    2013-01-01

    American-style summer programs in China, catering to Chinese-born students, have taken American universities by surprise. They are yet one more player in the complex and often opaque Chinese education industry, an industry in which American colleges are finding themselves increasingly entwined. These programs have become a booming enterprise,…

  11. Why Did They Sell My Loan?

    ERIC Educational Resources Information Center

    Clinton, Stephen W.

    1989-01-01

    The use of a student loan secondary market by a lender under the federally guaranteed Stafford Student Loan Program can be a shock to a student unfamiliar with the role secondary markets play. The first student loan secondary market (Student Loan Marketing Association, or Sallie Mae) was created in 1973. (MLW)

  12. Gentrifying Water and Selling Jim Crow.

    ERIC Educational Resources Information Center

    Williams, Brett

    2002-01-01

    Explores the gentrification of Washington, D.C.'s Anacostia watershed, tracing the projects of federal and local government to devitalize and demolish living black relationships and institutions while reifying lost, invented, or imagined communities. Explores connections among capital, community, culture, and state power. Examines problems in the…

  13. The Humanities: Why Such a Hard Sell?

    ERIC Educational Resources Information Center

    Ferrero, David J.

    2011-01-01

    Schools have traditionally had the goal of helping students grow in three areas: personal, economic, and civic. In recent years, however, the economic realm has taken precedence, with careers and credentials becoming a focus of education policy. In this article, Ferrero bewails the lack of support for the more personal and civic aims of education…

  14. Review Citations for Best-Selling Books.

    ERIC Educational Resources Information Center

    Palmer, Joseph W.

    1979-01-01

    Compares "Book Review Digest,""Book Review Index," and "Current Book Review Citations" for their coverage of titles appearing on the best-seller lists of "Publishers Weekly." Methodology is described and results are provided for both 1976 and 1978 titles. (Author/JD)

  15. Selling Workplace ESL Instructional Programs. ERIC Digest.

    ERIC Educational Resources Information Center

    Burt, Miriam

    Although basic skills and English language instruction are often viewed as real needs at the workplace, few companies provide this for their workers. Those that do are motivated by one or more of these factors: product quality improvement, commitment of top management to training and education, or the sales effort of an educational provider. Those…

  16. Revised State Budget Sells Kids Short

    ERIC Educational Resources Information Center

    Children Now, 2012

    2012-01-01

    The Administration's May Revision of the 2012-2013 state budget addresses a $15.7 billion shortfall through funding shifts, cuts, and new revenue sources that place children squarely in harms way. California's kids are already grossly underserved relative to the rest of the nation's children. If the May Revise budget is passed by the Legislature,…

  17. 29 CFR 779.241 - Selling.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... a practical sense is an essential part of consummating the “sale” of the particular goods, he will be considered to be “selling” the goods. “Selling” goods, under section 3(s) has reference only...

  18. Gene-to-metabolite network for biosynthesis of lignans in MeJA-elicited Isatis indigotica hairy root cultures

    PubMed Central

    Chen, Ruibing; Li, Qing; Tan, Hexin; Chen, Junfeng; Xiao, Ying; Ma, Ruifang; Gao, Shouhong; Zerbe, Philipp; Chen, Wansheng; Zhang, Lei

    2015-01-01

    Root and leaf tissue of Isatis indigotica shows notable anti-viral efficacy, and are widely used as “Banlangen” and “Daqingye” in traditional Chinese medicine. The plants' pharmacological activity is attributed to phenylpropanoids, especially a group of lignan metabolites. However, the biosynthesis of lignans in I. indigotica remains opaque. This study describes the discovery and analysis of biosynthetic genes and AP2/ERF-type transcription factors involved in lignan biosynthesis in I. indigotica. MeJA treatment revealed differential expression of three genes involved in phenylpropanoid backbone biosynthesis (IiPAL, IiC4H, Ii4CL), five genes involved in lignan biosynthesis (IiCAD, IiC3H, IiCCR, IiDIR, and IiPLR), and 112 putative AP2/ERF transcription factors. In addition, four intermediates of lariciresinol biosynthesis were found to be induced. Based on these results, a canonical correlation analysis using Pearson's correlation coefficient was performed to construct gene-to-metabolite networks and identify putative key genes and rate-limiting reactions in lignan biosynthesis. Over-expression of IiC3H, identified as a key pathway gene, was used for metabolic engineering of I. indigotica hairy roots, and resulted in an increase in lariciresinol production. These findings illustrate the utility of canonical correlation analysis for the discovery and metabolic engineering of key metabolic genes in plants. PMID:26579184

  19. The JaCVAM international validation study on the in vivo comet assay: Selection of test chemicals.

    PubMed

    Morita, Takeshi; Uno, Yoshifumi; Honma, Masamitsu; Kojima, Hajime; Hayashi, Makoto; Tice, Raymond R; Corvi, Raffaella; Schechtman, Leonard

    2015-07-01

    The Japanese Center for the Validation of Alternative Methods (JaCVAM) sponsored an international prevalidation and validation study of the in vivo rat alkaline pH comet assay. The main objective of the study was to assess the sensitivity and specificity of the assay for correctly identifying genotoxic carcinogens, as compared with the traditional rat liver unscheduled DNA synthesis assay. Based on existing carcinogenicity and genotoxicity data and chemical class information, 90 chemicals were identified as primary candidates for use in the validation study. From these 90 chemicals, 46 secondary candidates and then 40 final chemicals were selected based on a sufficiency of carcinogenic and genotoxic data, differences in chemical class or genotoxic or carcinogenic mode of action (MOA), availability, price, and ease of handling. These 40 chemicals included 19 genotoxic carcinogens, 6 genotoxic non-carcinogens, 7 non-genotoxic carcinogens and 8 non-genotoxic non-carcinogens. "Genotoxicity" was defined as positive in the Ames mutagenicity test or in one of the standard in vivo genotoxicity tests (primarily the erythrocyte micronucleus assay). These chemicals covered various chemicals classes, MOAs, and genotoxicity profiles and were considered to be suitable for the purpose of the validation study. General principles of chemical selection for validation studies are discussed.

  20. Extending MAM5 Meta-Model and JaCalIV E Framework to Integrate Smart Devices from Real Environments

    PubMed Central

    2016-01-01

    This paper presents the extension of a meta-model (MAM5) and a framework based on the model (JaCalIVE) for developing intelligent virtual environments. The goal of this extension is to develop augmented mirror worlds that represent a real and virtual world coupled, so that the virtual world not only reflects the real one, but also complements it. A new component called a smart resource artifact, that enables modelling and developing devices to access the real physical world, and a human in the loop agent to place a human in the system have been included in the meta-model and framework. The proposed extension of MAM5 has been tested by simulating a light control system where agents can access both virtual and real sensor/actuators through the smart resources developed. The results show that the use of real environment interactive elements (smart resource artifacts) in agent-based simulations allows to minimize the error between simulated and real system. PMID:26926691

  1. Extending MAM5 Meta-Model and JaCalIV E Framework to Integrate Smart Devices from Real Environments.

    PubMed

    Rincon, J A; Poza-Lujan, Jose-Luis; Julian, V; Posadas-Yagüe, Juan-Luis; Carrascosa, C

    2016-01-01

    This paper presents the extension of a meta-model (MAM5) and a framework based on the model (JaCalIVE) for developing intelligent virtual environments. The goal of this extension is to develop augmented mirror worlds that represent a real and virtual world coupled, so that the virtual world not only reflects the real one, but also complements it. A new component called a smart resource artifact, that enables modelling and developing devices to access the real physical world, and a human in the loop agent to place a human in the system have been included in the meta-model and framework. The proposed extension of MAM5 has been tested by simulating a light control system where agents can access both virtual and real sensor/actuators through the smart resources developed. The results show that the use of real environment interactive elements (smart resource artifacts) in agent-based simulations allows to minimize the error between simulated and real system.

  2. The Combined Effects of Ethylene and MeJA on Metabolic Profiling of Phenolic Compounds in Catharanthus roseus Revealed by Metabolomics Analysis.

    PubMed

    Liu, Jia; Liu, Yang; Wang, Yu; Zhang, Zhong-Hua; Zu, Yuan-Gang; Efferth, Thomas; Tang, Zhong-Hua

    2016-01-01

    Phenolic compounds belong to a class of secondary metabolites and are implicated in a wide range of responsive mechanisms in plants triggered by both biotic and abiotic elicitors. In this study, we approached the combinational effects of ethylene and MeJA (methyl jasmonate) on phenolic compounds profiles and gene expressions in the medicinal plant Catharanthus roseus. In virtue of a widely non-targeted metabolomics method, we identified a total of 34 kinds of phenolic compounds in the leaves, composed by 7 C6C1-, 11 C6C3-, and 16 C6C3C6 compounds. In addition, 7 kinds of intermediates critical for the biosynthesis of phenolic compounds and alkaloids were identified and discussed with phenolic metabolism. The combinational actions of ethylene and MeJA effectively promoted the total phenolic compounds, especially the C6C1 compounds (such as salicylic acid, benzoic acid) and C6C3 ones (such as cinnamic acid, sinapic acid). In contrast, the C6C3C6 compounds displayed a notably inhibitory trend in this case. Subsequently, the gene-to-metabolite networks were drawn up by searching for correlations between the expression profiles of 5 gene tags and the accumulation profiles of 41 metabolite peaks. Generally, we provide an insight into the controlling mode of ethylene-MeJA combination on phenolic metabolism in C. roseus leaves.

  3. OsNPR1 negatively regulates herbivore-induced JA and ethylene signaling and plant resistance to a chewing herbivore in rice.

    PubMed

    Li, Ran; Afsheen, Sumera; Xin, Zhaojun; Han, Xiu; Lou, Yonggen

    2013-03-01

    NPR1 (a non-expressor of pathogenesis-related genes1) has been reported to play an important role in plant defense by regulating signaling pathways. However, little to nothing is known about its function in herbivore-induced defense in monocot plants. Here, using suppressive substrate hybridization, we identified a NPR1 gene from rice, OsNPR1, and found that its expression levels were upregulated in response to infestation by the rice striped stem borer (SSB) Chilo suppressalis and rice leaf folder (LF) Cnaphalocrocis medinalis, and to mechanical wounding and treatment with jasmonic acid (JA) and salicylic acid (SA). Moreover, mechanical wounding induced the expression of OsNPR1 quickly, whereas herbivore infestation induced the gene more slowly. The antisense expression of OsNPR1 (as-npr1), which reduced the expression of the gene by 50%, increased elicited levels of JA and ethylene (ET) as well as of expression of a lipoxygenase gene OsHI-LOX and an ACC synthase gene OsACS2. The enhanced JA and ET signaling in as-npr1 plants increased the levels of herbivore-induced trypsin proteinase inhibitors (TrypPIs) and volatiles, and reduced the performance of SSB. Our results suggest that OsNPR1 is an early responding gene in herbivore-induced defense and that plants can use it to activate a specific and appropriate defense response against invaders by modulating signaling pathways.

  4. The Combined Effects of Ethylene and MeJA on Metabolic Profiling of Phenolic Compounds in Catharanthus roseus Revealed by Metabolomics Analysis

    PubMed Central

    Liu, Jia; Liu, Yang; Wang, Yu; Zhang, Zhong-Hua; Zu, Yuan-Gang; Efferth, Thomas; Tang, Zhong-Hua

    2016-01-01

    Phenolic compounds belong to a class of secondary metabolites and are implicated in a wide range of responsive mechanisms in plants triggered by both biotic and abiotic elicitors. In this study, we approached the combinational effects of ethylene and MeJA (methyl jasmonate) on phenolic compounds profiles and gene expressions in the medicinal plant Catharanthus roseus. In virtue of a widely non-targeted metabolomics method, we identified a total of 34 kinds of phenolic compounds in the leaves, composed by 7 C6C1-, 11 C6C3-, and 16 C6C3C6 compounds. In addition, 7 kinds of intermediates critical for the biosynthesis of phenolic compounds and alkaloids were identified and discussed with phenolic metabolism. The combinational actions of ethylene and MeJA effectively promoted the total phenolic compounds, especially the C6C1 compounds (such as salicylic acid, benzoic acid) and C6C3 ones (such as cinnamic acid, sinapic acid). In contrast, the C6C3C6 compounds displayed a notably inhibitory trend in this case. Subsequently, the gene-to-metabolite networks were drawn up by searching for correlations between the expression profiles of 5 gene tags and the accumulation profiles of 41 metabolite peaks. Generally, we provide an insight into the controlling mode of ethylene-MeJA combination on phenolic metabolism in C. roseus leaves. PMID:27375495

  5. Combination comet/micronucleus assay validation performed by BioReliance under the JaCVAM initiative.

    PubMed

    Pant, Kamala; Krsmanovic, Ljubica; Bruce, Shannon Wilson; Kelley, Tawney; Arevalo, Mirna; Atta-Safoh, Samuel; Debelie, Fekadu; La Force, Michelle L Klug; Springer, Sandra; Sly, Jamie; Paranjpe, Madhav; Lawlor, Timothy; Aardema, Marilyn

    2015-07-01

    In the international validation study of the in vivo rat alkaline comet assay (comet assay), the Japanese Center for the Validation of Alternative Methods (JaCVAM) provided three coded chemicals to BioReliance, 1,3-dichloropropene, ethionamide and busulfan, to be tested in a combined in vivo comet/micronucleus assay. Induction of DNA damage (comet) in liver, stomach and jejunum (1,3-dichloropropene only) cells, and induction of MNPCEs in bone marrow, were examined in male Sprague-Dawley (Hsd:SD) rats following oral administration of the test chemical for three consecutive days. A dose range finding (DRF) test was performed with each chemical to determine the maximum tolerated dose (MTD). Based on the results of the DRF test; 1,3-dichloropropene was tested at 50, 100 and 200 mg/kg/day; ethionamide was tested at 125, 250 and 500 mg/kg/day, and busulfan was tested at 10, 20 and 40 mg/kg/day. The results indicated that 1,3-dichloropropene induced DNA damage only in liver cells at all three test article doses, while no effects were observed in the stomach and jejunum cells. Additionally, it did not increase MNPCEs in the bone marrow. 1,3-Dichloropropene was concluded to be negative in the MN assay but positive in the comet assay. Ethionamide did not induce DNA damage in liver. However, in stomach, statistically significant decreases (although still within historical range) in % tail DNA at all test article doses compared to the vehicle control were observed. There was no increase in MNPCEs in the bone marrow. Thus, ethionamide was concluded to be negative in the comet/MN combined assay. Busulfan did not induce DNA damage in any of the organs tested (liver and stomach) but it did induce a significant increase in MNPCEs in the bone marrow. Busulfan was concluded to be negative in the comet assay but positive in the MN assay.

  6. Mineralogical characterization of tailing dams: incidence of abandoned mining works on soil pollution (Linares, Jaén)

    NASA Astrophysics Data System (ADS)

    de la Torre, M. J.; Hidalgo, C.; Rey, J.; Martínez, J.

    2012-04-01

    The metallogenic district of Linares-La Carolina (Jaén, Spain) consists of dyke mineralizations mainly of galena, accompanied by blende, chalcopyrite and barite. Associated to these abandoned mines, relatively extensive areas occupied by spoil heaps and tailing impoundments exist and constitute potential sources of soil pollution by metals and semimetals. In order to analyze the pollution potential of these mining wastes, we have carried out a mineralogical and geochemical study of seven tailing dams and surrounding soils in the area. The mineralogy of the samples was studied by x-ray diffraction (XRD) and scanning electron microscope (SEM). In addition, the total metal content of samples was determined by inductively coupled plasma mass spectrometry (ICP-MS) analysis. Samples were taken from the first 30 cm of the waste piles and soil deposits and white efflorescences were also obtained from the surface of the tailings. In all analyzed heaps, high to very high total contents in Pb (1220-22890 mg/kg), Zn (150-51280 mg/kg), Mn (2658-4160 mg/kg), Ba (1026-19610 mg/kg) and Fe (19400-138000 mg/kg) were observed. The concentrations for these same elements in the studied soils range from 527-9900 mg/kg for Pb, 27-1700 mg/kg for Zn, 506-2464 mg/kg for Mn, 2832-4306 for Ba and 8642-29753 mg/kg for Fe, and these figures indicate a contamination of the soils, according to the guidelines established by the Spanish law. The XRD and SEM results indicate that the tailings are primarily constituted by gangue of the exploited mineralization: quartz, calcite, ankerite, feldspars and phyllosilicates. They are inherited, primary mineral phases. Galena, also primary, appears in low proportion, as well as lepidocrocite, melanterite and cerussite, being these three last secondary minerals and indicating a certain remobilization of metal cations, especially lead and iron. On the other hand, quartz and phyllosilicates predominate in the soils, in which, in addition, is identified a

  7. Sell or not to sell - a pastoralist's dilemma: a lesson from the slaughterhouse

    SciTech Connect

    Perevolotsky, A.

    1986-09-01

    Marketing strategies employed by herders in Piura, a coastal region in northern Peru, are discussed in conjunction with ecological and economic factors. Data from regional slaughterhouses' help in analyzing decisions concerning livestock offtakes and in understanding the rationale behind them. Piura is characterized by sharp weather changes, amplified by the El Nino phenomenon. The variations in precipitation and forage availability strongly affect production, structure, and dynamics of the herds and are therefore a significant component in determining the actual marketing decision strategy. Pure herders adopt an insurance strategy emphasizing a build-up of herds during abundant years in order to compensate for considerable losses caused by frequent droughts. The use of stubble and concentrated feed in coping with droughts is restricted by economic and social factors. Agropastoralists are affected not only by ecological factors but also by labor availability, agricultural cash requirements, and household needs. As a result, these goat keepers practice a capital-manipulation strategy in which goats and kids are used as a form of capital. The paper provides an empirical representation of these two marketing strategies based on slaughterhouses' records and a complementary field survey.

  8. PP2C-like Promoter and Its Deletion Variants Are Induced by ABA but Not by MeJA and SA in Arabidopsis thaliana.

    PubMed

    Bhalothia, Purva; Sangwan, Chetna; Alok, Anshu; Mehrotra, Sandhya; Mehrotra, Rajesh

    2016-01-01

    Gene expression is mediated through interaction between cis regulatory elements and its cognate transcription factors. Cis regulatory elements are defined as non-coding DNA sequences that provide the binding sites for transcription factors and are clustered in the upstream region of genes. ACGT cis regulatory element is one of the important cis regulatory elements found to be involved in diverse biological processes like auxin response, salicylic acid (SA) response, UV light response, ABA response and jasmonic acid (JA) response. We identified through in silico analysis that the upstream region of protein phosphatase 2C (PP2C) gene has a distinct genetic architecture of ACGT elements. In the present study, the activation of the full length promoter and its deletion constructs like 900 base pair, 500 base pair, 400 base pair and NRM (Nathji Rajesh Mehrotra) were examined by stable transformation in Arabidopsis thaliana using β-glucuronidase as the reporter gene. Evaluation of deletion constructs of PP2C-like promoter was carried out in the presence of phytohormones like abscisic acid (ABA), SA and JA. Our result indicated that the full length and 900 base pair promoter-reporter constructs of PP2C-like promoter was induced in response to ABA but not to methyl jasmonate and SA.

  9. Pro Memoria. Professor Bolesław Jałowy (1906-1943): Mortui viventes obligant - the livings are obligated to the dead.

    PubMed

    Wincewicz, Andrzej

    2016-01-01

    Professor Bolesław Jałowy (1906-1943) was a chairman of Department of Histology and Embryology at Faculty of Medicine of King John Casimir University (Polish: Universytet Jana Kazimierza: UJK) in Lvov. He succeeded Professor Władysław Szymonowicz (1869-1939) who held this position for decades. As the most skillful followers of his tutor, Bolesław Jałowy was a great investigator of physiology of human tissue, embryogenesis, histological consequences of female sex hormones on blood clotting action as well as regeneration of nerves in addition to description of silver staining technique for reticulin fibers of skin. He was a hard working person with gentle attitude to such a subtle matter as microscopic structure of human body. However, he happened to live in brutal conditions of nationalistic struggles. His example shows how much a dedicated scientist could do in a very short time as his life was tragically ended with murdering him during World War Two. His story is a great lesson for generations of academic workers how to meet high moral standards with efficient and creative scientific work in evil and destructive, nationalistic climate that occurs usually in wartime.

  10. Investigation of sodium arsenite, thioacetamide, and diethanolamine in the alkaline comet assay: Part of the JaCVAM comet validation exercise.

    PubMed

    Beevers, Carol; Henderson, Debbie; Lillford, Lucinda

    2015-07-01

    As part of the Japanese Center for the Validation of Alternative Methods (JaCVAM)-initiative international validation study of the in vivo rat alkaline comet assay (comet assay), we examined sodium arsenite, thioacetamide, and diethanolamine. Using the JaCVAM approved study protocol version 14.2, each chemical was tested in male rats up to maximum tolerated dose levels and DNA damage in the liver and stomach was assessed approximately 3h after the final administration by gavage. Histopathology assessments of liver and stomach sections from the same animals were also examined for evidence of cytotoxicity or necrosis. No evidence of DNA damage was observed in the stomach of animals treated with sodium arsenite at 7.5, 15, or 30 mg/kg/day. However, equivocal findings were found in the liver, where increases in DNA migration were observed in two independent experiments, but not in all treated animals and not at the same dose levels. Thioacetamide caused an increase in DNA migration in the stomach of rats treated at 19, 38, and 75 mg/kg/day, but not in the liver, despite evidence of marked hepatotoxicity following histopathology assessments. No evidence of DNA damage was observed in the stomach or liver of animals treated with diethanolamine at 175, 350, or 700 mg/kg/day.

  11. Hypogean pseudoscorpions (Arachnida) from Jaén province (Andalusia, Spain), with descriptions of four new species and a new synonymy.

    PubMed

    Zaragoza, Juan A; Pérez, Toni

    2013-01-01

    Four new hypogean species are described from the Jaén province (southern Spain): Chthonius (Ephippiochthonius) espa- nyoli sp. nov., C. (E.) giennensis sp. nov., C. (E.) villacarrillo sp. nov. and Neobisium (Ommatoblothrus) perezruizi sp. nov. New records are given for the species Chthonius (E.) cazorlensis, C. (E.) perezi, C. (E.) tetrachelatus, Neobisium (O.) perezi, Microcreagrella caeca caeca and Allochernes masi. Chthonius (E.) verai and C. (E.) minutus are removed from the list of the Andalusian fauna. A new synonymy is proposed: Neobisium (O.) gev Carabajal Márquez, García Carrillo & Rodríguez Fernández, 2011, is a junior subjective synonym of N. (O.) perezi Carabajal Márquez, García Carrillo & Rodríguez Fernández, 2011.

  12. Monitoring of selected priority and emerging contaminants in the Guadalquivir River and other related surface waters in the province of Jaén, South East Spain.

    PubMed

    Robles-Molina, José; Gilbert-López, Bienvenida; García-Reyes, Juan F; Molina-Díaz, Antonio

    2014-05-01

    The province of Jaén counts with four natural parks, numerous rivers, reservoirs and wetlands; moreover, it is probably the region with higher olive oil production in the world, which makes this zone a proper target to be studied based on the European Water Framework Directive 2000/60/CE. The aim of this survey is to monitor a total number of 373 compounds belonging to different families (pesticides, PAHs, nitrosamines, drugs of abuse, pharmaceuticals and life-style compounds) in surface waters located at different points of the province of Jaén. Among these compounds some priority organic substances (regulated by the EU Directive 2008/105/EC) and pollutants of emerging concern (not regulated yet) can be found. A liquid chromatography electrospray time-of-flight mass spectrometry (LC-TOFMS) method covering 340 compounds was developed and applied, together with a gas chromatography triple-quadrupole mass spectrometry (GC-MS/MS) method which enabled the analysis of 63 organic contaminants (30 of these compounds are analyzed by LC-TOFMS as well). From April 2009 to November 2010 a total of 83 surface water samples were collected (rivers, reservoirs and wetlands). In this period numerous organic contaminants were detected, most of them at the ng L(-1) level. The most frequently priority substances found were chlorpyrifos ethyl, diuron and hexachlorobenzene. Within the other groups, the most frequently detected compounds were: terbuthylazine, oxyfluorfen, desethyl terbuthylazine, diphenylamine (pesticide family); fluorene, phenanthrene, pyrene (PAHs group), codeine, paracetamol (pharmaceuticals compounds) and caffeine, nicotine (life-style compounds). As is could be expected, the total concentration of emerging contaminants is distinctly larger than that of priority pollutants, highlighting the importance of continuing with the study of their presence, fate and effects in aquatic environments. However, concentration levels (at the ng per liter level) are low in

  13. Molecular characterisation of extended-spectrum β-lactamase- and plasmid AmpC-producing Escherichia coli strains isolated from broilers in Béjaïa, Algeria.

    PubMed

    Belmahdi, Mohamed; Bakour, Sofiane; Al Bayssari, Charbel; Touati, Abdelaziz; Rolain, Jean-Marc

    2016-09-01

    This study aimed to characterise the molecular support of antibiotic resistance in expanded-spectrum cephalosporin (ESC)-resistant Escherichia coli isolates recovered from healthy broilers in Béjaïa, northeast Algeria. A total of 61 intestinal swabs from slaughtered broilers from four regions in Béjaïa locality, Algeria, were collected between February and April 2014, from which 20 ESC-resistant E. coli strains were isolated. Escherichia coli isolates were identified by classical biochemical and MALDI-TOF methods. Antibiotic susceptibility testing was performed using disk diffusion and Etest methods. Screening for β-lactamases, aminoglycoside-modifying enzyme (AME)-encoding genes and qnr determinants was performed by PCR and sequencing. Clonal relatedness was determined using molecular typing by multilocus sequence typing (MLST). Antibiotic susceptibility testing revealed that the isolates showed high rates of resistance (>90%) to amoxicillin, amoxicillin/clavulanic acid, piperacillin/tazobactam, aztreonam, ceftazidime, streptomycin, tobramycin, nalidixic acid and ciprofloxacin. Low rates of resistance were observed for kanamycin (35%), amikacin (30%), cefoxitin (20%) and cefotaxime (15%). Molecular characterisation revealed that all of the isolates expressed the blaTEM-1 gene. Fourteen of them harboured the blaSHV-12 gene, two harboured the blaCTX-M-1 gene and four isolates harboured blaCMY-2. Screening for AME-encoding genes demonstrated that all isolates contained the aadA gene. In addition, qnrA was detected as the quinolone resistance determinant in 13 isolates. MLST revealed four known sequence types (STs), including ST744, ST38, ST1011 and ST2179, as well as one new sequence type (ST5086). Here we report the first study describing the clonal diversity of extended-spectrum β-lactamase (ESBL)- and plasmid AmpC-producing E. coli isolated from healthy broilers in Algeria.

  14. Re-analysis results using medians of the data from the JaCVAM-organized international validation study of the in vivo rat alkaline comet assay.

    PubMed

    Uno, Yoshifumi; Omori, Takashi

    2015-07-01

    The data from the JaCVAM-organized international validation study of the in vivo rat alkaline comet assay were reported and analyzed statistically using the simple means of % tail DNA. However, OECD test guideline TG 489 recommends use of the median for data analysis due to the hierarchical nature of the data. Comparison between the simple mean approach and the median based approach for positive/negative/equivocal chemical calls was conducted using the % tail DNA data for the 40 chemicals tested in the JaCVAM-organized international validation study of the in vivo rat alkaline comet assay, using liver and stomach as target organs. In the liver, two genotoxic chemicals, o-anisidine and 9-aminoacridine hydrochloride monohydrate, were positive using the median based approach but negative using the simple mean approach, and two genotoxic chemicals, 2-acetylaminofluorene and busulfan were equivocal using the median based approach but negative using the simple mean approach. In contrast, cadmium chloride (genotoxic carcinogen) was equivocal in both organs using the median based approach, while positive and equivocal in liver and stomach, respectively, using the simple mean approach. Two data sets of sodium arsenite showed equivocal and negative results for liver using the median based approach, although both data sets were equivocal using the simple mean approach. Overall, there are no large differences in terms of the genotoxic call between both approaches. However, the median based approach recommended in OECD TG 489 has an advantage toward higher precision within the groups treated with a test chemical, whereas the approach might show the lower values for the effect.

  15. Variability of phenotype, anthocyanin indexes, and flavonoids in accessions from a close relative of soybean, Neontonia wightii (Wright & Arn. J.A. Lackey) in the U.S. germplasm collection for potential use as a health forage

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A close relative of soybean, Neonotonia wightii (Wright & Arn. J.A. Lackey), is used as a ruminant feed and restores soil productivity in Brazil and Zimbabwe, respectively. Neonotonia wightii accessions were grown in a greenhouse at Griffin, Georgia, characterized for various phenotypic traits, and...

  16. Simulated herbivory in chickpea causes rapid changes in defense pathways and hormonal transcription networks of JA/ethylene/GA/auxin within minutes of wounding

    PubMed Central

    Pandey, Saurabh Prakash; Srivastava, Shruti; Goel, Ridhi; Lakhwani, Deepika; Singh, Priya; Asif, Mehar Hasan; Sane, Aniruddha P.

    2017-01-01

    Chickpea (C. arietinum L.) is an important pulse crop in Asian and African countries that suffers significant yield losses due to attacks by insects like H. armigera. To obtain insights into early responses of chickpea to insect attack, a transcriptomic analysis of chickpea leaves just 20 minutes after simulated herbivory was performed, using oral secretions of H. armigera coupled with mechanical wounding. Expression profiles revealed differential regulation of 8.4% of the total leaf transcriptome with 1334 genes up-regulated and 501 down-regulated upon wounding at log2-fold change (|FC| ≤ −1 and ≥1) and FDR value ≤ 0.05. In silico analysis showed the activation of defenses through up-regulation of genes of the phenylpropanoid pathway, pathogenesis, oxidases and CYTP450 besides differential regulation of kinases, phosphatases and transcription factors of the WRKY, MYB, ERFs, bZIP families. A substantial change in the regulation of hormonal networks was observed with up-regulation of JA and ethylene pathways and suppression of growth associated hormone pathways like GA and auxin within 20 minutes of wounding. Secondary qPCR comparison of selected genes showed that oral secretions often increased differential expression relative to mechanical damage alone. The studies provide new insights into early wound responses in chickpea. PMID:28300183

  17. LOW-TEMPERATURE ION TRAP STUDIES OF N{sup +}({sup 3} P{sub ja} ) + H{sub 2}(j) {yields} NH{sup +} + H

    SciTech Connect

    Zymak, I.; Hejduk, M.; Mulin, D.; Plasil, R.; Glosik, J.; Gerlich, D.

    2013-05-01

    Using a low-temperature 22-pole ion trap apparatus, detailed measurements for the title reaction have been performed between 10 K and 100 K in order to get some state specific information about this fundamental hydrogen abstraction process. The relative population of the two lowest H{sub 2} rotational states, j = 0 and 1, has been varied systematically. NH{sup +} formation is nearly thermo-neutral; however, to date, the energetics are not known with the accuracy required for low-temperature astrochemistry. Additional complications arise from the fact that, so far, there is no reliable theoretical or experimental information on how the reactivity of the N{sup +} ion depends on its fine-structure (FS) state {sup 3} P{sub ja} . Since in the present trapping experiment, thermalization of the initially hot FS population competes with hydrogen abstraction, the evaluation of the decay of N{sup +} ions over long storage times and at various He and H{sub 2} gas densities provides information on these processes. First assuming strict adiabatic behavior, a set of state specific rate coefficients is derived from the measured thermal rate coefficients. In addition, by recording the disappearance of the N{sup +} ions over several orders of magnitude, information on nonadiabatic transitions is extracted including FS-changing collisions.

  18. Hydrogeological research on intensively exploited deep aquifers in the `Loma de Úbeda' area (Jaén, southern Spain)

    NASA Astrophysics Data System (ADS)

    González-Ramón, Antonio; Rodríguez-Arévalo, Javier; Martos-Rosillo, Sergio; Gollonet, Javier

    2013-06-01

    The intensive use of groundwater for irrigation in the area of Úbeda (`Loma de Úbeda', Jaén, southern Spain) has transformed an area of traditionally rain-fed dry farmland into fields with some of the highest olive oil productivity in the world. Early hydrogeological research studies, initiated just after the beginning of the groundwater exploitation, revealed that the water was collected from three different overlapping aquifers occupying an area of over 1,100 km2, with the lower aquifers located at depths from 300 to over 700 m in an area of 440 km2. Multidisciplinary research, based on geological characterization, and piezometric, hydrochemical and isotopic data, has led to a conceptual model of functioning in this complex hydrogeological system. The proposed model allows for the identification of the recharge areas, and the discharge, which is at present mainly associated with the groundwater pumping. Areas of mixing of waters from the different aquifers and the main hydrogeochemical processes affecting groundwater quality are described.

  19. Simulated herbivory in chickpea causes rapid changes in defense pathways and hormonal transcription networks of JA/ethylene/GA/auxin within minutes of wounding.

    PubMed

    Pandey, Saurabh Prakash; Srivastava, Shruti; Goel, Ridhi; Lakhwani, Deepika; Singh, Priya; Asif, Mehar Hasan; Sane, Aniruddha P

    2017-03-16

    Chickpea (C. arietinum L.) is an important pulse crop in Asian and African countries that suffers significant yield losses due to attacks by insects like H. armigera. To obtain insights into early responses of chickpea to insect attack, a transcriptomic analysis of chickpea leaves just 20 minutes after simulated herbivory was performed, using oral secretions of H. armigera coupled with mechanical wounding. Expression profiles revealed differential regulation of 8.4% of the total leaf transcriptome with 1334 genes up-regulated and 501 down-regulated upon wounding at log2-fold change (|FC| ≤ -1 and ≥1) and FDR value ≤ 0.05. In silico analysis showed the activation of defenses through up-regulation of genes of the phenylpropanoid pathway, pathogenesis, oxidases and CYTP450 besides differential regulation of kinases, phosphatases and transcription factors of the WRKY, MYB, ERFs, bZIP families. A substantial change in the regulation of hormonal networks was observed with up-regulation of JA and ethylene pathways and suppression of growth associated hormone pathways like GA and auxin within 20 minutes of wounding. Secondary qPCR comparison of selected genes showed that oral secretions often increased differential expression relative to mechanical damage alone. The studies provide new insights into early wound responses in chickpea.

  20. Physical and metabolic interactions of Pseudomonas sp. strain JA5-B45 and Rhodococcus sp. strain F9-D79 during growth on crude oil and effect of a chemical surfactant on them.

    PubMed

    Van Hamme, J D; Ward, O P

    2001-10-01

    Methods to enhance crude oil biodegradation by mixed bacterial cultures, for example, (bio)surfactant addition, are complicated by the diversity of microbial populations within a given culture. The physical and metabolic interactions between Rhodococcus sp. strain F9-D79 and Pseudomonas sp. strain JA5-B45 were examined during growth on Bow River crude oil. The effects of a nonionic chemical surfactant, Igepal CO-630 (nonylphenol ethoxylate), also were evaluated. Strain F9-D79 grew attached to the oil-water interface and produced a mycolic acid-containing capsule. Crude oil emulsification and surface activity were associated with the cellular fraction. Strain JA5-B45 grew in the aqueous phase and was unable to emulsify oil, but cell-free supernatants mediated kerosene-water emulsion formation. In coculture, stable emulsions were formed and strain JA5-B45 had an affinity for the capsule produced by strain F9-D79. Igepal CO-630 inhibited F9-D79 cells from adhering to the interface, and cells grew dispersed in the aqueous phase as 0.5-microm cocci rather than 2.5-microm rods. The surfactant increased total petroleum hydrocarbon removal by strain JA5-B45 from 4 to 22% and included both saturated compounds and aromatics. In coculture, TPH removal increased from 13 to 40% following surfactant addition. The culture pH normally increased from 7.0 to between 7.5 and 8.5, although addition of Igepal CO-630 to F9-D79 cultures resulted in a drop to pH 5.5. We suggest a dual role for the nonylphenol ethoxylate surfactant in the coculture: (i) to improve hydrocarbon uptake by strain JA5-B45 through emulsification and (ii) to prevent strain F9-D79 from adhering to the oil-water interface, indirectly increasing hydrocarbon availability. These varied effects on hydrocarbon biodegradation could explain some of the known diversity of surfactant effects.

  1. Physical and Metabolic Interactions of Pseudomonas sp. Strain JA5-B45 and Rhodococcus sp. Strain F9-D79 during Growth on Crude Oil and Effect of a Chemical Surfactant on Them

    PubMed Central

    Van Hamme, Jonathan D.; Ward, Owen P.

    2001-01-01

    Methods to enhance crude oil biodegradation by mixed bacterial cultures, for example, (bio)surfactant addition, are complicated by the diversity of microbial populations within a given culture. The physical and metabolic interactions between Rhodococcus sp. strain F9-D79 and Pseudomonas sp. strain JA5-B45 were examined during growth on Bow River crude oil. The effects of a nonionic chemical surfactant, Igepal CO-630 (nonylphenol ethoxylate), also were evaluated. Strain F9-D79 grew attached to the oil-water interface and produced a mycolic acid-containing capsule. Crude oil emulsification and surface activity were associated with the cellular fraction. Strain JA5-B45 grew in the aqueous phase and was unable to emulsify oil, but cell-free supernatants mediated kerosene-water emulsion formation. In coculture, stable emulsions were formed and strain JA5-B45 had an affinity for the capsule produced by strain F9-D79. Igepal CO-630 inhibited F9-D79 cells from adhering to the interface, and cells grew dispersed in the aqueous phase as 0.5-μm cocci rather than 2.5-μm rods. The surfactant increased total petroleum hydrocarbon removal by strain JA5-B45 from 4 to 22% and included both saturated compounds and aromatics. In coculture, TPH removal increased from 13 to 40% following surfactant addition. The culture pH normally increased from 7.0 to between 7.5 and 8.5, although addition of Igepal CO-630 to F9-D79 cultures resulted in a drop to pH 5.5. We suggest a dual role for the nonylphenol ethoxylate surfactant in the coculture: (i) to improve hydrocarbon uptake by strain JA5-B45 through emulsification and (ii) to prevent strain F9-D79 from adhering to the oil-water interface, indirectly increasing hydrocarbon availability. These varied effects on hydrocarbon biodegradation could explain some of the known diversity of surfactant effects. PMID:11571196

  2. JaCVAM-organized international validation study of the in vivo rodent alkaline comet assay for detection of genotoxic carcinogens: II. Summary of definitive validation study results.

    PubMed

    Uno, Yoshifumi; Kojima, Hajime; Omori, Takashi; Corvi, Raffaella; Honma, Masamistu; Schechtman, Leonard M; Tice, Raymond R; Beevers, Carol; De Boeck, Marlies; Burlinson, Brian; Hobbs, Cheryl A; Kitamoto, Sachiko; Kraynak, Andrew R; McNamee, James; Nakagawa, Yuzuki; Pant, Kamala; Plappert-Helbig, Ulla; Priestley, Catherine; Takasawa, Hironao; Wada, Kunio; Wirnitzer, Uta; Asano, Norihide; Escobar, Patricia A; Lovell, David; Morita, Takeshi; Nakajima, Madoka; Ohno, Yasuo; Hayashi, Makoto

    2015-07-01

    The in vivo rodent alkaline comet assay (comet assay) is used internationally to investigate the in vivo genotoxic potential of test chemicals. This assay, however, has not previously been formally validated. The Japanese Center for the Validation of Alternative Methods (JaCVAM), with the cooperation of the U.S. NTP Interagency Center for the Evaluation of Alternative Toxicological Methods (NICEATM)/the Interagency Coordinating Committee on the Validation of Alternative Methods (ICCVAM), the European Centre for the Validation of Alternative Methods (ECVAM), and the Japanese Environmental Mutagen Society/Mammalian Mutagenesis Study Group (JEMS/MMS), organized an international validation study to evaluate the reliability and relevance of the assay for identifying genotoxic carcinogens, using liver and stomach as target organs. The ultimate goal of this exercise was to establish an Organisation for Economic Co-operation and Development (OECD) test guideline. The study protocol was optimized in the pre-validation studies, and then the definitive (4th phase) validation study was conducted in two steps. In the 1st step, assay reproducibility was confirmed among laboratories using four coded reference chemicals and the positive control ethyl methanesulfonate. In the 2nd step, the predictive capability was investigated using 40 coded chemicals with known genotoxic and carcinogenic activity (i.e., genotoxic carcinogens, genotoxic non-carcinogens, non-genotoxic carcinogens, and non-genotoxic non-carcinogens). Based on the results obtained, the in vivo comet assay is concluded to be highly capable of identifying genotoxic chemicals and therefore can serve as a reliable predictor of rodent carcinogenicity.

  3. Comparative genomic analysis of single-molecule sequencing and hybrid approaches for finishing the Clostridium autoethanogenum JA1-1 strain DSM 10061 genome

    SciTech Connect

    Brown, Steven D; Nagaraju, Shilpa; Utturkar, Sagar M; De Tissera, Sashini; Segovia, Simón; Mitchell, Wayne; Land, Miriam L; Dassanayake, Asela; Köpke, Michael

    2014-01-01

    Background Clostridium autoethanogenum strain JA1-1 (DSM 10061) is an acetogen capable of fermenting CO, CO2 and H2 (e.g. from syngas or waste gases) into biofuel ethanol and commodity chemicals such as 2,3-butanediol. A draft genome sequence consisting of 100 contigs has been published. Results A closed, high-quality genome sequence for C. autoethanogenum DSM10061 was generated using only the latest single-molecule DNA sequencing technology and without the need for manual finishing. It is assigned to the most complex genome classification based upon genome features such as repeats, prophage, nine copies of the rRNA gene operons. It has a low G + C content of 31.1%. Illumina, 454, Illumina/454 hybrid assemblies were generated and then compared to the draft and PacBio assemblies using summary statistics, CGAL, QUAST and REAPR bioinformatics tools and comparative genomic approaches. Assemblies based upon shorter read DNA technologies were confounded by the large number repeats and their size, which in the case of the rRNA gene operons were ~5 kb. CRISPR (Clustered Regularly Interspaced Short Paloindromic Repeats) systems among biotechnologically relevant Clostridia were classified and related to plasmid content and prophages. Potential associations between plasmid content and CRISPR systems may have implications for historical industrial scale Acetone-Butanol-Ethanol (ABE) fermentation failures and future large scale bacterial fermentations. While C. autoethanogenum contains an active CRISPR system, no such system is present in the closely related Clostridium ljungdahlii DSM 13528. A common prophage inserted into the Arg-tRNA shared between the strains suggests a common ancestor. However, C. ljungdahlii contains several additional putative prophages and it has more than double the amount of prophage DNA compared to C. autoethanogenum. Other differences include important metabolic genes for central metabolism (as an additional hydrogenase and the absence of a

  4. Reporting on Literacy: Soft-Selling a Complex Political Story.

    ERIC Educational Resources Information Center

    Moss, Michael

    Much of the story of illiteracy is about the powerless, and reporting on it can focus on who lacks power and why. However, much of the untold story about illiteracy is about people with power who are choosing not to wield it--educators, economists, elected officials, presidential candidates, corporate leaders, publishers, and the literate public…

  5. Struggling Colleges Debate the Propriety of Selling Their Art

    ERIC Educational Resources Information Center

    Strout, Erin

    2007-01-01

    Donors give art collections worth millions of dollars to colleges, mostly to be displayed in their art museums. But some financially strapped colleges have started looking at the art as a way of escaping their financial woes. Fisk University expects to make $16-million from the sale of two paintings, one by Georgia O'Keeffe and another by Marsden…

  6. Social marketing of condoms: selling protection and changing behavior.

    PubMed

    Townsend, S

    1991-06-01

    Social marketing of condoms in Zaire began in 1987 and sales rose to 8 million in 1990, a notable change from the previous situation where about half a million condoms were distributed by government health clinics only. Social marketing is the use of for-profit sales and marketing techniques for public health problem.s When the Zaire National AIDS Committee initiated social marketing of condoms, with the assistance of Population Services International, they had the experience of successful Asian programs of the 1970s, and the political sanction resulting from the AIDS threat to back them up. Efforts were made to find just the right product name, "Prudence," logo, package, color and slogan by consumer research. Prudence implies responsible behavior, capturing both the AIDS and STD prevention and the family planning markets. Consumers like the package and associate it with quality, since most condoms sold before in Zaire had no special packaging. Distribution outlets include 7000 retail shops, groceries, pharmacies, hotel, social clubs, 300 bars and even Congo River barges which sex workers frequent. The price was set close to that of a pack of gum for 3, or that of a bottle of beer for a dozen. Promotion is limited by a government ban of advertising in mass media, so point of purchase materials, special offers and promotional items are being used. Prudence condoms are now being marketed in Cameroon and Burundi.

  7. Creating and selling embryos for "donation": ethical challenges.

    PubMed

    Klitzman, Robert; Sauer, Mark V

    2015-02-01

    The commercial creation and sale of embryos has begun, which poses a series of ethical questions that have received little scholarly attention. Some of the concerns that arise are similar to those posed by the sale of gametes, while other issues differ markedly. Questions emerge, first, regarding the rights of the unborn children and their ability to know their biological parents. Companies that create human embryos de novo may wish to keep gamete providers anonymous. Many of these offspring thus will never learn that their parents are not their biologic parents. Yet, such disclosures, regarding not only one but both of these biologic parents, may be important for these individuals; and a lack of this knowledge may impede their physical and psychological health. Second, questions surface regarding the fees that providers should charge for embryos and whether these amounts should vary based on the traits of 1 or both of the gamete donors. Some prospective parents may seek specific traits in a baby (eg, height or eye/hair coloring), which prompts the creation of embryos from 2 gamete donors who possess these characteristics. Third, ownership of embryos created without an advanced directive by patients poses dilemmas (eg, disposition of any remaining embryos). Fourth, guidelines do not yet exist to limit the number of embryos sold from each pair of gamete donors. Hence, unbeknownst to each other, full siblings could potentially meet, get married, and procreate. This discussion has several critical implications for future practice and professional education and policy. Patients with diseases associated with genetic tests may well ask obstetricians, gynecologists, and other physicians about these techniques and practices. Clinicians can refer such patients to assisted reproductive technology specialists; however, familiarity with the basic aspects of the issues and complexities involved could aid these providers and their patients Several of these issues can be addressed relatively easily through guidelines from professional associations (eg, limiting the number of embryos sold from each pair of gamete donors). Because creation and sales of embryos will likely spread, consideration of appropriate responses is critical to establish standards of care to help the future offspring, and ensure ongoing public trust.

  8. Critical Thinking as Miracle Tonic: Selling Snake Oil in Education.

    ERIC Educational Resources Information Center

    Hyslop-Margison, Emery J.

    This paper proposes that the current interest in critical thinking is based on important conceptual, epistemological, and procedural confusions. It suggests that the attempt to identify a successful critical thinking construct mirrors the search for miracle tonics often peddled by snake oil salesmen as a medicinal cure-all. It goes to suggest that…

  9. An Honors Koan: Selling Water by the River

    ERIC Educational Resources Information Center

    Portnoy, Jeffrey A.

    2013-01-01

    Since Jerry Herron begins his forum essay, "Notes toward an Excellent Marxist-Elitist Honors Admissions Policy," with his anecdotal True Genealogical Confessions, Jeffery Portnoy, writes here that he feels feel obligated to begin in a similar mode. One side of Portnoy's family was in the real estate business in St. Louis, and the other…

  10. The Bioethics and Utility of Selling Kidneys for Renal Transplantation

    PubMed Central

    Berman, Elisheva; Lipschutz, Jonathan M.; Bloom, Roy D.; Lipschutz, Joshua H.

    2008-01-01

    In the fifty years since kidney transplantation was first performed, this procedure has evolved from a highly speculative biomedical endeavor to a medically viable and often standard course of therapy (1). Long-term survival is markedly improved among patients who receive a kidney compared with patients who remain on the waiting list for such an organ (2). As outcomes have improved and more clinical indications have emerged, the number of people awaiting transplantation has grown significantly. In stark contrast to the robust expansion of the waiting list, the number of available deceased donors has remained relatively constant over the last several years (1). The current mechanism for procuring kidneys relies on voluntary donations by the general public, with the primary motivation being altruism. However, in light of the ever-increasing waiting list, it is the authors’ belief that the current system needs to be revised if supply is ever going to meet demand. In response to this critical organ shortage, different programs have been developed in an attempt to increase organ donation. At present, however, no solution to the problem has emerged. This paper begins by outlining the scope of the problem and current legislation governing the procurement of transplantable organs/tissues in the United States. It continues with an overview of different proposals to increase supply. It concludes by exploring some of the controversy surrounding the proposal to increase donation using financial incentives. Though the following discussion certainly has implications for other transplantable organs, this paper will focus on kidney transplantation because the waiting list for kidneys is by far the longest of all the solid organs; and, as it carries the smallest risk to living donors, is the least ethically problematic. PMID:18589084

  11. Franklin Delano Roosevelt's Successful Selling of the Destroyer Deal.

    ERIC Educational Resources Information Center

    Williams, Thomas E.; Morgan, Hugh

    One of the most evident examples of President Franklin D. Roosevelt's mastery of the press and his manipulation of public opinion was the 1940 arrangement of the transfer of 50 United States naval destroyers to Great Britain while that nation was at war with Germany and Italy and while noninterventionist sentiment was quite strong in the United…

  12. Instrumentational complexity of music genres and why simplicity sells.

    PubMed

    Percino, Gamaliel; Klimek, Peter; Thurner, Stefan

    2014-01-01

    Listening habits are strongly influenced by two opposing aspects, the desire for variety and the demand for uniformity in music. In this work we quantify these two notions in terms of instrumentation and production technologies that are typically involved in crafting popular music. We assign an 'instrumentational complexity value' to each music style. Styles of low instrumentational complexity tend to have generic instrumentations that can also be found in many other styles. Styles of high complexity, on the other hand, are characterized by a large variety of instruments that can only be found in a small number of other styles. To model these results we propose a simple stochastic model that explicitly takes the capabilities of artists into account. We find empirical evidence that individual styles show dramatic changes in their instrumentational complexity over the last fifty years. 'New wave' or 'disco' quickly climbed towards higher complexity in the 70s and fell back to low complexity levels shortly afterwards, whereas styles like 'folk rock' remained at constant high instrumentational complexity levels. We show that changes in the instrumentational complexity of a style are related to its number of sales and to the number of artists contributing to that style. As a style attracts a growing number of artists, its instrumentational variety usually increases. At the same time the instrumentational uniformity of a style decreases, i.e. a unique stylistic and increasingly complex expression pattern emerges. In contrast, album sales of a given style typically increase with decreasing instrumentational complexity. This can be interpreted as music becoming increasingly formulaic in terms of instrumentation once commercial or mainstream success sets in.

  13. Selling and Building Linked Data: Drive Value and Gain Momentum

    NASA Astrophysics Data System (ADS)

    Harris, Kristen

    Data inside enterprises is exploding. Routinely key decision makers state, "one of the most valuable assets of our organization is actionable information." Paradoxically, it is often challenging to gather the necessary metrics to build a business case to justify a Linked Data initiative for improved data quality in the face of the well-documented shortcomings of traditional enterprise approaches to data management. Internet standards have matured considerably in the last decade. The number of linked data sets is growing daily and already exceeds well in excess of ten thousand data sets, over 400 of which are published via the US Government at gov as of April 2010. Many of these linked data sets are high quality. Best practices for linking data within the enterprise are increasingly being published in articles, blogs and technical books. One of the pioneering linked enterprise data projects was undertaken by a Fortune 500 company in the early 2000 timeframe. The lessons learned about how to navigate the management and organizational dynamics are relevant today. This chapter outlines the successful strategies for a linked enterprise data initiative, including a consistent metadata management strategy across lines of business, definition and documentation of data ownership and the value of cross-functional teams in the definition, development and deployment of the project.

  14. Students Selling Sex: Marketisation, Higher Education and Consumption

    ERIC Educational Resources Information Center

    Sanders, Teela; Hardy, Kate

    2015-01-01

    Robust academic research on the topic of students involved in the sex industry is in its infancy, yet the relationship appears consistent and permanent. This paper draws on findings from the largest study into the stripping industry in the United Kingdom to explore the relationships between students, sex work and consumption. To make sense of the…

  15. Selling the Work Ethic: From Puritan Pulpit to Corporate PR.

    ERIC Educational Resources Information Center

    Beder, Sharon

    This book provides an account and critique of the work ethic, a central aspect of modern capitalist society. Chapter 1 is an introduction. Chapter 2 considers social changes in Europe in the 16th-17th centuries, maintaining that work was imbued with moral qualities and wealth seeking acquired social legitimacy through religious sanction. Chapter 3…

  16. Selling University Reform: The University of Melbourne and the Press

    ERIC Educational Resources Information Center

    Potts, Anthony

    2012-01-01

    Since the advent of the "Times Higher Education Supplement World University Rankings" and the "Academic Rankings of World Universities" by Shanghai Jiao Tong University, some Australian universities have become especially concerned with being ranked among the 100 leading universities. The University of Melbourne, Australia's…

  17. Fatal Attraction: The Selling of Addiction. Special Double Issue.

    ERIC Educational Resources Information Center

    Silver, Rosalind, Ed.; Thoman, Elizabeth, Ed.

    1991-01-01

    This issue of "Media & Values" provides essays and teaching ideas for addressing the influence of the media in society and the growing incidence of addiction. Articles in this issue include: (1) "Culture of Addiction" (Rosalind Silver); (2) "Crack and the Box" (Pete Hamill); (3) "When It Comes to Drugs, Beware the Censor's Fix" (David Musto); (4)…

  18. Cigar magazines: using tobacco to sell a lifestyle

    PubMed Central

    Wenger, L.; Malone, R.; George, A.; Bero, L.

    2001-01-01

    OBJECTIVE—To assess the content of two cigar "lifestyle" magazines, Cigar Aficionado and Smoke.
DESIGN—Content analysis of cigar focused articles.
SUBJECTS—Cigar focused articles (n = 353) from Cigar Aficionado and Smoke magazines.
MAIN OUTCOME MEASURES—Primary focus; mention of health effects, environmental tobacco smoke, or scientific research; quotation and description of individuals; characteristics such as sex, age, ethnicity, smoking status, affiliation, and stance towards cigars; and overall image of cigars.
RESULTS—Cigar business-focused articles were the largest category (40%, n = 143), followed by articles about cigar events (12%, n = 42). Notable were articles featuring cigar benefits to raise money for health charities. Celebrities were featured in 34% (n = 121) of articles and 96% (n = 271) favoured cigar use. Only four (1%) articles featured health effects of cigars as a primary focus.
CONCLUSIONS—Cigar Aficionado and Smoke broke new ground in tobacco marketing by combining promotion of product, lifestyle, and industry in the same vehicle and linking the medium directly to product related events that extended its reach. The creation and marketing of new tobacco use sites challenges the increasing "isolation" of smokers, and positions cigar use as a socially welcome relief from restrictions. Public health advocates should anticipate and challenge other new tobacco marketing vehicles as communications technologies advance and public spaces for smoking shrink.


Keywords: cigars; cigar magazines; lifestyles; tobacco marketing PMID:11544394

  19. 48 CFR 1631.205-75 - Selling costs.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... EMPLOYEES HEALTH BENEFITS ACQUISITION REGULATION GENERAL CONTRACTING REQUIREMENTS CONTRACT COST PRINCIPLES... modified to eliminate from allowable costs those costs related to sales promotion and the payment of sales... for attendance at Open Season Health fairs and other similar activities at which carriers...

  20. 48 CFR 1631.205-75 - Selling costs.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... EMPLOYEES HEALTH BENEFITS ACQUISITION REGULATION GENERAL CONTRACTING REQUIREMENTS CONTRACT COST PRINCIPLES... modified to eliminate from allowable costs those costs related to sales promotion and the payment of sales... for attendance at Open Season Health fairs and other similar activities at which carriers...

  1. 48 CFR 1631.205-75 - Selling costs.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... EMPLOYEES HEALTH BENEFITS ACQUISITION REGULATION GENERAL CONTRACTING REQUIREMENTS CONTRACT COST PRINCIPLES... modified to eliminate from allowable costs those costs related to sales promotion and the payment of sales... for attendance at Open Season Health fairs and other similar activities at which carriers...

  2. The Commission Game: An Ethics Activity for Professional Selling Courses

    ERIC Educational Resources Information Center

    Milewicz, Chad

    2012-01-01

    The Commission Game is an experiment-based experiential learning activity designed to elicit students' sincere ethical decision making in an ambiguous sales context. The activity includes multiple relevant stakeholders as well as tangible, shared risk/reward elements. The activity's design encourages students to contemplate their own personal code…

  3. Why Honors Is a Hard Sell in the Community College

    ERIC Educational Resources Information Center

    Engelen-Eigles, Deborah; Milner, Janice Levinsohn

    2014-01-01

    At the college president's request and after attending the 2013 NCHC conference in New Orleans, the authors developed a comprehensive honors program framework and spent the spring semester of 2014 sharing it with units across the college for discussion and feedback. The response was overwhelmingly positive. Despite the many benefits of this…

  4. Sell lumens, not kilowatts: The future for electric utilities

    SciTech Connect

    Piepmeier, J.M. ); Jermain, D. ); Egnor, T.L. )

    1993-04-01

    The key to the future for electric utilities will not be found in legislation or regulation. Title VII of the Energy Policy Act of 1992 will prove to be just as ineffectual in improving the industry's position as was the Public Utility Regulatory Policies Act of 1978. These legislative palliatives, which produced so much commotion and so many reams of heated commentary, are largely irrelevant to a successful future for electric utilities. The key will be found in economics, not in law, and the future will lie in completing Thomas A. Edison's century-old vision for the industry, half of which the industry has heretofore ignored. The industry must embrace the complete vision and evolve from electric utilities into [open quotes]end-use energy utilities.[close quotes

  5. Selling green power in California: Product, industry, and market trends

    SciTech Connect

    Wiser, R.H.; Pickle, S.J.

    1998-05-01

    As one of the first US stages to open its doors to retail electric competition, California offers an important opportunity to assess the effectiveness of green power marketing as a mechanism for supporting renewable energy. This report is an interim assessment of key green power product, industry, and market trends in California. The report identifies and analyzes: the potential size of the green power market in California; the companies participating in the green power market; the green power products being offered and their prices; the impact of the green market on renewable generators and the environment; and the influence of several public policies and non-governmental programs on the market for green power. Data used in this paper have been collected, in large part, from surveys and interviews with green power marketers that took place between December 1997 and April 1998. There remain legitimate concerns over the viability of green power marketing to support significant quantities of renewable energy and provide large environmental gains, and it is far too early to assess the overall strength of customer demand for renewable energy. A critical finding of this report is that, because of the high cost of acquiring and servicing residential customers and the low utility default service price, green power marketing affords new energy service providers one of the only viable entrees to California`s residential marketplace.

  6. Argentina: An emerging marketer is set to sell itself

    SciTech Connect

    1997-01-01

    The nuclear industry in Argentina is outlined in the article. A brief history of the industry`s transformation from a federal to a private enterprise is presented. Information on fuel cycle facilities for uranium production, uranium dioxide production, zircalloy turbine production, nuclear fuel fabrication, and heavy water production is provided. Other topics presented include spent fuel storage, technology marketing, industry restructuring, and bilateral weapons agreements.

  7. "I have seen the future": selling the unsustainable city.

    PubMed

    Hayden, Dolores

    2012-01-01

    Seventy years ago, General Motors’ Highways and Horizons exhibit at the World’s Fair, designed by Norman Bel Geddes and Eero Saarinen, promoted demand for cars and federal highways without any concern for environmental sustainability, the theme of our 2010 conference. The main exhibit included a sequence of four parts (entrance ramps, map lobby, Futurama ride, and “intersection of 1960”) where the viewer’s perception of spatial scale was manipulated. Setha M. Low’s theory of “embodied space” helps decode why movement through these diverse spaces influenced millions of Americans’ views of transportation and urban form, a promotional success yet to be equaled by advocates of environmental sustainability.

  8. Selling the story: Narratives and charisma in adults with TBI

    PubMed Central

    JONES, CORINNE A.; TURKSTRA, LYN S.

    2015-01-01

    Objective To examine storytelling performance behaviours in adults with traumatic brain injury (TBI) and relate these behaviours to perceived charisma and desirability as a conversation partner. Design and methods Seven adult males with traumatic brain injury (TBI) told their accident narratives to a male confederate. Ten male undergraduate students rated 1-minute video clips from the beginning of each narrative using the Charismatic Leadership Communication Scale (CLCS). Raters also indicated whether or not they would like to engage in conversation with each participant. Results Of the performative behaviours analysed, gestures alone significantly influenced CLCS ratings and reported likelihood of engaging in future conversation with the participant. Post-hoc analysis revealed that speech rate was significantly correlated with all of the preceding measures. There was a significant correlation between self- and other-ratings of charisma. Conclusions The findings suggest that aspects of non-verbal performance, namely gesture use and speech rate, influence how charismatic an individual is perceived to be and how likely someone is to engage in conversation with that person. Variability in these performance behaviours may contribute to the variation in social outcomes seen in the TBI population. PMID:21714624

  9. Effects of Advertising on Advance Selling and Online Search

    ERIC Educational Resources Information Center

    Joo, Mingyu

    2012-01-01

    Advertising has been one of the most important marketing variables for both practices and academic literature, and it has been generally known to raise the firm's market share (Ataman, van Heerde, and Mela 2010). However, under the contemporary market environment, advertising's impact may be more complicated. For example, in advance…

  10. The Selling of Clio: American History College Survey Textbooks.

    ERIC Educational Resources Information Center

    Chesson, Michael B.

    1988-01-01

    Reviews the treatment of 15 different, often unrelated topics and eras, as found in the first volumes of 19 widely-used U.S. history survey textbooks. Topics reviewed include Jamestown, American slavery, the Constitution, and Fort Sumter. The review focuses on factual errors, questionable interpretations, boring prose, and the question of…

  11. Do Sell-Side Stock Analysts Exhibit Escalation of Commitment?

    PubMed Central

    Milkman, Katherine L.

    2010-01-01

    This paper presents evidence that when an analyst makes an out-of-consensus forecast of a company’s quarterly earnings that turns out to be incorrect, she escalates her commitment to maintaining an out-of-consensus view on the company. Relative to an analyst who was close to the consensus, the out-of-consensus analyst adjusts her forecasts for the current fiscal year’s earnings less in the direction of the quarterly earnings surprise. On average, this type of updating behavior reduces forecasting accuracy, so it does not seem to reflect superior private information. Further empirical results suggest that analysts do not have financial incentives to stand by extreme stock calls in the face of contradictory evidence. Managerial and financial market implications are discussed. PMID:21516220

  12. The bioethics and utility of selling kidneys for renal transplantation.

    PubMed

    Berman, E; Lipschutz, J M; Bloom, R D; Lipschutz, J H

    2008-06-01

    In the 53 years since kidney transplantation was first performed, this procedure has evolved from a highly speculative biomedical endeavor to a medically viable and often standard course of therapy. Long-term survival is markedly improved among patients who receive a kidney compared with patients who remain on the waiting list for such an organ. As outcomes have improved and more clinical indications have emerged, the number of people awaiting transplantation has grown significantly. In stark contrast to the robust expansion of the waiting list, the number of available deceased donors has remained relatively constant over the last several years. The current mechanism for procuring kidneys relies on voluntary donations by the general public, with the primary motivation being altruism. However, in light of the ever-increasing waiting list, it is the researchers' belief that the current system needs to be revised if supply is ever going to meet demand. In response to this critical organ shortage, different programs have been developed in an attempt to increase organ donation. At present, however, no solution to the problem has emerged. This report begins by outlining the scope of the problem and current legislation governing the procurement of transplantable organs/tissues in the United States. It continues with an overview of different proposals to increase supply. It concludes by exploring some of the controversy surrounding the proposal to increase donation using financial incentives. Though the following discussion certainly has implications for other transplantable organs, this report focuses on kidney transplantation because the waiting list for kidneys is by far the longest of all waiting lists for solid organs; and, as kidney transplant carries the smallest risk to living donors, it is the least ethically problematic.

  13. 32 CFR 507.7 - Authority to sell.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... RELATIONS MANUFACTURE AND SALE OF DECORATIONS, MEDALS, BADGES, INSIGNIA, COMMERCIAL USE OF HERALDIC DESIGNS AND HERALDIC QUALITY CONTROL PROGRAM Manufacture and Sale of Decorations, Medals, Badges, and...

  14. Knowing what to sell, when, and to whom.

    PubMed

    Kumar, V; Venkatesan, Rajkumar; Reinartz, Werner

    2006-03-01

    Despite an abundance of data, most companies do a poor job of predicting the behavior of their customers. In fact, the authors' research suggests that even companies that take the greatest trouble over their predictions about whether a particular customer will buy a particular product are correct only around 55% of the time--a result that hardly justifies the costs of having a CRM system in the first place. Businesses usually conclude from studies like this that it's impossible to use the past to predict the future, so they revert to the timeworn marketing practice of inundating their customers with offers. But as the authors explain, the reason for the poor predictions is not any basic limitation of CRM systems or the predictive power of past behavior, but rather of the mathematical methods that companies use to interpret the data. The authors have developed a new way of predicting customer behavior, based on the work of the Nobel Prize-winning economist Daniel McFadden, that delivers vastly improved results. Indeed, the methodology increases the odds of successfully predicting a specific purchase by a specific customer at a specific time to about 85%, a number that will have a major impact on any company's marketing ROI. What's more, using this methodology, companies can increase revenues while reducing their frequency of customer contact-evidence that overcommunication with customers may actually damage a company's sales.

  15. "Creative solutions": selling cigarettes in a smoke-free world

    PubMed Central

    Smith, E; Malone, R

    2004-01-01

    Objective: To analyse the development and execution of the "Creative Solutions" Benson & Hedges advertising campaign to understand its social, political, and commercial implications. Methods: Searches of the Philip Morris documents and Legacy Tobacco Documents websites for relevant materials; Lexis/Nexis searches of major news and business publications; and denotative and connotative analyses of the advertising imagery. Results: Philip Morris developed the Creative Solutions campaign in an effort to directly confront the successes of the tobacco control movement in establishing new laws and norms that promoted clean indoor air. The campaign's imagery attempted to help smokers and potential smokers overcome the physical and social downsides of smoking cigarettes by managing risk and resolving internal conflict. The slogans suggested a variety of ways for smokers to respond to restrictions on their habit. The campaign also featured information about the Accommodation Program, Philip Morris's attempt to organise opposition to clean indoor air laws. Conclusion: The campaign was a commercial failure, with little impact on sales of the brand. Philip Morris got some exposure for the Accommodation Program and its anti-regulatory position. The lack of commercial response to the ads suggests that they were unable to successfully resolve the contradictions that smokers were increasingly experiencing and confirms the power of changing social norms to counter tobacco industry tactics. PMID:14985598

  16. Does Mother Nature Really Sell Margarine?: The Uncertain Rural Future.

    ERIC Educational Resources Information Center

    Schwartz, Peter

    There exists a question as to whether United States society and its agricultural system will continue to follow the long-term trend of Western civilization or move in a new direction. The "modernization trend" of Western civilization has 4 components: scientification of knowledge, secularization of human values, industrialization of…

  17. Advertising the "New Fun-Tier": Selling Casinos to Consumers

    ERIC Educational Resources Information Center

    McMullan, John L.; Miller, Delthia

    2010-01-01

    There is an absence of research on the commercial advertising of gambling as it relates to casinos. This study examines print, radio, television and point of sale casino ads that aired in Canada. Using quantitative and qualitative methods we analyze a convenience sample of 367 ads that aired or were printed in 2005 and 2006. Our findings indicate…

  18. Urania in the Marketplace: The Selling of Mt. Palomar

    NASA Astrophysics Data System (ADS)

    Rumstay, Kenneth S.

    2016-01-01

    The 200-inch Hale telescope atop Mt. Palomar is one of the most iconic scientific facilities ever constructed. The world's largest optical telescope for over a quarter-century, it served as a symbol of hope for America during the Great Depression and in the post-war years. In 2016 we celebrate the eightieth anniversaries of the completion of the mirror blank, the start of construction of the dome and mounting, and the beginning of astronomical research at Palomar Observatory by Fritz Zwicky (with the 18-inch Schmidt camera).During its construction, and for many years after "first light" in 1949, the Hale telescope was prominently featured in numerous magazine advertisements. Most of these represented companies directly involved in its construction, notably Corning Glass Works, which was justly proud of its magnificent accomplishment. But companies only vaguely linked to the project, or not at all, also co-opted the mystique of "the World's Largest Eye" to promote their goods or services. Surprisingly, in light of the fact that it bore responsibility for fabricating the complex and innovative mounting, the Westinghouse Electric & Manufacturing Company appears to have run only a single advertisement (in The National Geographic Magazine) touting its contribution to the project.Examples of magazine advertisements spanning the period 1936 to 1959 are presented.This work was supported by a faculty scholarship grant from Valdosta State University.

  19. Selling the Art Program: Tickling a Sacred Cow.

    ERIC Educational Resources Information Center

    Macklin, Anderson D.

    1978-01-01

    For art to survive in the schools, a new image must be presented to the public. Art should be distinguished from crafts, which have a purely recreational connotation, and promoted as a utilitarian subject, teaching fundamental production skills leading toward mastery, as the Suzuki method does in music. (SJL)

  20. Selling Your Design: Oral Communication Pedagogy in Design Education

    ERIC Educational Resources Information Center

    Morton, Janne; O'Brien, David

    2005-01-01

    Good design skills are the main focus of assessment practices in design education and are evaluated primarily by drawings and models. In some settings, design studio pedagogy tends to reflect only these content-oriented assessment priorities, with minimal attention paid to the development of oral communication skills. Yet, in many professional…

  1. Protecting Your District's Ability to Sell Bonds or Other Debt

    ERIC Educational Resources Information Center

    Warden, Dan; Palsma, Abigail Stokes

    2012-01-01

    If a school district has more than $10 million in tax-exempt debt, inclusive of all outstanding bonds, certificates of participation, and other public debt issues with durations of more than nine months, it must comply with the contractual requirements entered into under Securities and Exchange Commission (SEC) rules. District administrators and…

  2. Vo-Ed's Role in Selling Solar Power

    ERIC Educational Resources Information Center

    Larson, Milton E.; Valentine, Ivan E.

    1976-01-01

    Speaks to the role of vocational education in providing massive training programs to prepare technicians and other service personnel to perform installation and service functions necessary to support architects and engineers designing and developing solar energy systems. (HD)

  3. Selling the OR: strategies to compete in the marketplace.

    PubMed

    Lee, M G

    1989-07-01

    1. OR directors are expected to be successful business managers in addition to their usual management responsibilities. 2. To complete with other health-care facilities, it is essential to incorporate marketing as part of the management strategy. 3. Marketing includes developing the skill level of all staff members, including middle managers, staff nurses, and ancillary personnel. 4. OR directors must create a work environment where the physician and staff are partners in providing quality patient care.

  4. Creating and Selling Embryos for “Donation”: Ethical Challenges

    PubMed Central

    Klitzman, Robert; Sauer, Mark V.

    2015-01-01

    The commercial creation and sale of embryos has begun, posing a series of ethical questions that have received little scholarly attention. Some of the concerns that arise are similar to those posed by the sale of gametes, while other issues differ markedly. Questions emerge, firstly, regarding the rights of the unborn children – their ability to know their biological parents. Companies that create human embryos de novo may wish to keep gamete providers anonymous. Many of these offspring will thus never learn that their parents are not their biological parents. Yet, such disclosures – regarding not only one, but both of these biological parents – may be important for these individuals; and lack of this knowledge may impede their physical and psychological health. Secondly, questions surface regarding the fees that providers should charge for embryos, and whether these amounts should vary based on the traits of one or both of the gamete donors. Some prospective parents may seek specific traits in a baby (e.g., height or eye/hair coloring), prompting creation of embryos from two gamete donors who possess these characteristics. Thirdly, ownership of embryos created without an advanced directive by patients poses dilemmas – e.g., disposition of any remaining embryos. Fourthly, guidelines do not yet exist to limit the number of embryos sold from each pair of gamete donors. Hence, unbeknownst to each other, full siblings could potentially meet, get married and procreate. This discussion has several critical implications for future practice, and professional education and policy. Patients with diseases associated with genetic tests may well ask obstetricians, gynecologists and other physicians about these techniques and practices. Clinicians can refer such patients to Assisted Reproductive Technology specialists, but familiarity with the basic aspects of the issues and complexities involved could aid themselves and their patients Several of these issues can be relatively easily addressed through guidelines from professional associations (e.g., limiting the number of embryos sold from each pair of gamete donors). As creation and sales of embryos will likely spread, consideration of appropriate responses is critical in order to establish standards of care to help the future offspring, and ensure ongoing public trust. PMID:25448512

  5. You Weld It--We'll Sell It

    ERIC Educational Resources Information Center

    Branchick, Michael; Shettler, Monte

    1972-01-01

    The marketing and vocational welding classes of our high school were brought together in an effort to prepare the students for a realistic look at industry and marketing through the formation of a corporation. (Author/JS)

  6. How to Become an Effective Advocate without Selling Your Soul

    ERIC Educational Resources Information Center

    Grob, George F.

    2014-01-01

    The primary focus of this article is ''advocating for the results of a specific evaluation.'' To some extent, all evaluators are advocates, however the author notes, "there is no great mystery in how to use evaluations for advocacy, especially with respect to influencing policy making within federal and state…

  7. Selling a Story: How to Write a Successful Press Release

    NASA Astrophysics Data System (ADS)

    Pearson, E.

    2016-12-01

    Press releases are an important part of communicating new discoveries with the general public. However, if they are confusing or difficult to read then they are unlikely to be picked up by media outlets. This article details how to create a successful press release by addressing a series of points: learning how to identify the audience; writing text that is both eye catching and clear; including multimedia and contact details; getting your press release to the media; and timing.

  8. Selling School Budgets in Hard Times. Fastback 215.

    ERIC Educational Resources Information Center

    Goldstein, William

    Financial planning for education is a difficult task as schools struggle for a share of diminishing tax resources. The presentation of school budgets for voter approval must be done as effectively as possible. When presenting budgets, administrators should take into account that the public has a low tolerance for detail, budgets are boring, a well…

  9. Instrumentational Complexity of Music Genres and Why Simplicity Sells

    PubMed Central

    Percino, Gamaliel; Klimek, Peter; Thurner, Stefan

    2014-01-01

    Listening habits are strongly influenced by two opposing aspects, the desire for variety and the demand for uniformity in music. In this work we quantify these two notions in terms of instrumentation and production technologies that are typically involved in crafting popular music. We assign an ‘instrumentational complexity value’ to each music style. Styles of low instrumentational complexity tend to have generic instrumentations that can also be found in many other styles. Styles of high complexity, on the other hand, are characterized by a large variety of instruments that can only be found in a small number of other styles. To model these results we propose a simple stochastic model that explicitly takes the capabilities of artists into account. We find empirical evidence that individual styles show dramatic changes in their instrumentational complexity over the last fifty years. ‘New wave’ or ‘disco’ quickly climbed towards higher complexity in the 70s and fell back to low complexity levels shortly afterwards, whereas styles like ‘folk rock’ remained at constant high instrumentational complexity levels. We show that changes in the instrumentational complexity of a style are related to its number of sales and to the number of artists contributing to that style. As a style attracts a growing number of artists, its instrumentational variety usually increases. At the same time the instrumentational uniformity of a style decreases, i.e. a unique stylistic and increasingly complex expression pattern emerges. In contrast, album sales of a given style typically increase with decreasing instrumentational complexity. This can be interpreted as music becoming increasingly formulaic in terms of instrumentation once commercial or mainstream success sets in. PMID:25551631

  10. Selling yourself: Titmuss's argument against a market in blood.

    PubMed

    Archard, David

    2002-01-01

    This article defends Richard Titmuss's argument, and Peter Singer's sympathetic support for it, against orthodox philosophical criticism. The article specifies the sense in which a market in blood is "dehumanising" as having to do with a loss of "imagined community" or social "integration," and not with a loss of valued or "deeper" liberty. It separates two "domino arguments"--the "contamination of meaning" argument and the "erosion of motivation" argument--which support, in different but interrelated ways, the claim that a market in blood is "imperialistic." Concentrating on the first domino argument the article considers the view that monetary and non-monetary meanings of the same good can co-exist given the robustness of certain kinds of relationship and joint undertakings within which gifts can figure. It argues that societal relationships are vulnerable or permeable to the effects of the market in a way that those constitutive of the personal sphere are not. General, more broadly political questions remain unanswered but the core of Titmuss's original and challenging argument remains and can be presented in a defensible form.

  11. Selling Futures: A New Image for Australian Universities?

    ERIC Educational Resources Information Center

    Symes, Colin

    1996-01-01

    This article argues that as a result of the changes in Australian higher education, which emphasize economic accountability, a more instrumental university has emerged. This policy shift is reflected in university promotional material and marketing strategies that indicate the extent to which this instrumental ethos pervades higher education and…

  12. Selling yourself in a tough health care market.

    PubMed

    Kennedy, M M

    1999-01-01

    Physician executives are vying for top positions in health care organizations. With so many qualified candidates in the marketplace, how can you differentiate yourself as a strong contender? Recruiters search through hundreds of résumés, not the ten or 20 that many hospital and HMO CEOs have seen. Recruiters--the people who actually find specific candidates--were interviewed to find out what they are looking for. Here are the top items on their checklists: Articulated focus, documented results, competitive intelligence, professional growth, good references, appearance and social skills, responsive and timely, and technical skills.

  13. Polymer therapeutics: Top 10 selling pharmaceuticals - what next?

    PubMed

    Duncan, Ruth

    2014-09-28

    At the time of the first issue of the Journal of Controlled Release (JCR), polymeric drugs, polymer-drug and protein conjugates and block copolymer micelles carrying bound drugs, i.e. polymer therapeutics, were still regarded as scientific curiosities with little or no prospect of generating practical to use medicines. How this perception has changed. Many major Pharma now have R&D programmes in this area and in 2013 two polymer therapeutics, Copaxone and Neulasta, are featured in the Top 10 US pharmaceutical sales list. Although there are a growing number of marketed products (e.g. PEGylated proteins, a PEG-aptamer and oral polymeric sequestrants), and the first follow-on (generic products) are emerging, the first polymer-drug conjugates and block copolymer micelle products (as covalent conjugates) have yet to enter routine clinical use. Industrial familiarity and recent advances in the underpinning scientific disciplines will no doubt accelerate the transfer of polymer therapeutics into clinically useful medicines and imaging agents. This short personal perspective reflects on the current status of polymer therapeutics and the future opportunities to improve their successful translation. It adds to recent and historical reviews that comprehensively document the evolution of the field since JCR was born.

  14. Use of Native American imagery to sell tobacco.

    PubMed

    BlueEye, LaDonna

    2004-05-01

    The tobacco plant has been used in Native American communities in sacred, traditional and cultural ways prior to the arrival of Christopher Columbus. Early immigrants to North America were introduced to tobacco use by Native Americans and the plant was quickly turned into a commercial success in Europe. Native American images have been used for centuries to commercialize a product that once held only spiritual significance to American Indian tribes. This article provides a history of tobacco, from the sacred use by Native Americans, to the commercialism of tobacco in North America. Included is a review of early to current advertisements using Native American images.

  15. Citrus leprosis virus C Infection Results in Hypersensitive-Like Response, Suppression of the JA/ET Plant Defense Pathway and Promotion of the Colonization of Its Mite Vector

    PubMed Central

    Arena, Gabriella D.; Ramos-González, Pedro L.; Nunes, Maria A.; Ribeiro-Alves, Marcelo; Camargo, Luis E. A.; Kitajima, Elliot W.; Machado, Marcos A.; Freitas-Astúa, Juliana

    2016-01-01

    Leprosis is a serious disease of citrus caused by Citrus leprosis virus C (CiLV-C, genus Cilevirus) whose transmission is mediated by false spider mites of the genus Brevipalpus. CiLV-C infection does not systemically spread in any of its known host plants, thus remaining restricted to local lesions around the feeding sites of viruliferous mites. To get insight into this unusual pathosystem, we evaluated the expression profiles of genes involved in defense mechanisms of Arabidopsis thaliana and Citrus sinensis upon infestation with non-viruliferous and viruliferous mites by using reverse-transcription qPCR. These results were analyzed together with the production of reactive oxygen species (ROS) and the appearance of dead cells as assessed by histochemical assays. After interaction with non-viruliferous mites, plants locally accumulated ROS and triggered the salicylic acid (SA) and jasmonate/ethylene (JA/ET) pathways. ERF branch of the JA/ET pathways was highly activated. In contrast, JA pathway genes were markedly suppressed upon the CiLV-C infection mediated by viruliferous mites. Viral infection also intensified the ROS burst and cell death, and enhanced the expression of genes involved in the RNA silencing mechanism and SA pathway. After 13 days of infestation of two sets of Arabidopsis plants with non-viruliferous and viruliferous mites, the number of mites in the CiLV-C infected Arabidopsis plants was significantly higher than in those infested with the non-viruliferous ones. Oviposition of the viruliferous mites occurred preferentially in the CiLV-C infected leaves. Based on these results, we postulated the first model of plant/Brevipalpus mite/cilevirus interaction in which cells surrounding the feeding sites of viruliferous mites typify the outcome of a hypersensitive-like response, whereas viral infection induces changes in the behavior of its vector. PMID:27933078

  16. Buckwheat (Fagopyrum esculentum M.) sprout treated with methyl jasmonate (MeJA) improved anti-adipogenic activity associated with the oxidative stress system in 3T3-L1 adipocytes.

    PubMed

    Lee, Young-Jun; Kim, Kui-Jin; Park, Kee-Jai; Yoon, Bo-Ra; Lim, Jeong-Ho; Lee, Ok-Hwan

    2013-01-11

    Buckwheat sprouts contain various bioactive compounds including rutin which have a number of biological activities. We have previously shown that buckwheat sprouts (TBWE) treated with methyl jasmonate (MeJA) significantly increased the amount of phenolics and the antioxidant activity. The aim of this study was to demonstrate the effect of TBWE on anti-adipogenesis and pro-oxidant enzyme in 3T3-L1 adipocytes. We also evaluated the anti-oxidative activity of TBWE in adipocytes by using the nitroblue tetrazolium assay. Our data showed that TBWE markedly inhibited adipocyte differentiation and ROS production in 3T3-L1 cells compared with control groups. Moreover, TBWE has strongly shown the inhibition of adipogenic transcription factor as well as pro-oxidant enzymes. Together, we demonstrate that the MeJA treatment significantly increased the amount of phenolic compound, resulting in the suppression of adipogenesis and ROS production in the 3T3-L1 cells. These findings indicate that TBWE has the potential for anti-adipogenesis activity with anti-oxidative properties.

  17. Induced Systemic Resistance against Botrytis cinerea by Bacillus cereus AR156 through a JA/ET- and NPR1-Dependent Signaling Pathway and Activates PAMP-Triggered Immunity in Arabidopsis

    PubMed Central

    Nie, Pingping; Li, Xia; Wang, Shune; Guo, Jianhua; Zhao, Hongwei; Niu, Dongdong

    2017-01-01

    Induced resistance response is a potent and cost effective plant defense against pathogen attack. The effectiveness and underlying mechanisms of the suppressive ability by Bacillus cereus AR156 to Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) in Arabidopsis has been investigated previously; however, the strength of induced systemic resistance (ISR) activity against Botrytis cinerea remains unknown. Here, we show that root-drench application of AR156 significantly reduces disease incidence through activation of ISR. This protection is accompanied with multilayered ISR defense response activated via enhanced accumulation of PR1 protein expression in a timely manner, hydrogen peroxide accumulation and callose deposition, which is significantly more intense in plants with both AR156 pretreatment and B. cinerea inoculation than that in plants with pathogen inoculation only. Moreover, AR156 can trigger ISR in sid2-2 and NahG mutants, but not in jar1, ein2 and npr1 mutant plants. Our results indicate that AR156-induced ISR depends on JA/ET-signaling pathway and NPR1, but not SA. Also, AR156-treated plants are able to rapidly activate MAPK signaling and FRK1/WRKY53 gene expression, both of which are involved in pathogen associated molecular pattern (PAMP)-triggered immunity (PTI). The results indicate that AR156 can induce ISR by the JA/ET-signaling pathways in an NPR1-dependent manner and involves multiple PTI components. PMID:28293243

  18. Induced Systemic Resistance against Botrytis cinerea by Bacillus cereus AR156 through a JA/ET- and NPR1-Dependent Signaling Pathway and Activates PAMP-Triggered Immunity in Arabidopsis.

    PubMed

    Nie, Pingping; Li, Xia; Wang, Shune; Guo, Jianhua; Zhao, Hongwei; Niu, Dongdong

    2017-01-01

    Induced resistance response is a potent and cost effective plant defense against pathogen attack. The effectiveness and underlying mechanisms of the suppressive ability by Bacillus cereus AR156 to Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) in Arabidopsis has been investigated previously; however, the strength of induced systemic resistance (ISR) activity against Botrytis cinerea remains unknown. Here, we show that root-drench application of AR156 significantly reduces disease incidence through activation of ISR. This protection is accompanied with multilayered ISR defense response activated via enhanced accumulation of PR1 protein expression in a timely manner, hydrogen peroxide accumulation and callose deposition, which is significantly more intense in plants with both AR156 pretreatment and B. cinerea inoculation than that in plants with pathogen inoculation only. Moreover, AR156 can trigger ISR in sid2-2 and NahG mutants, but not in jar1, ein2 and npr1 mutant plants. Our results indicate that AR156-induced ISR depends on JA/ET-signaling pathway and NPR1, but not SA. Also, AR156-treated plants are able to rapidly activate MAPK signaling and FRK1/WRKY53 gene expression, both of which are involved in pathogen associated molecular pattern (PAMP)-triggered immunity (PTI). The results indicate that AR156 can induce ISR by the JA/ET-signaling pathways in an NPR1-dependent manner and involves multiple PTI components.

  19. Four new species of Pomphopsilla Jałoszyński and a new record of Cephennodes glabella Castellini in the Democratic Republic of the Congo
    (Coleoptera: Staphylinidae: Scydmaeninae).

    PubMed

    Jałoszyński, Paweł

    2016-04-07

    Four new species of the genus Pomphopsilla Jałoszyński are described, all based on specimens collected in the north-eastern part of DR Congo: P. pygmaea sp. n., P. pseudosoror sp. n., P. similis sp. n., and P. gumovskyi sp. n. Previously Pomphopsilla, represented by two species, was known to occur in Kenya; the only genera of Cephenniini recorded from DR Congo were Cephennodes Reitter and Cephennomicrus Reitter. Cephennodes (s. str.) glabella Castellini, so far known only from the type series, is recorded from the same region of Congo, based on numerous specimens collected by yellow pan traps. Because figures in the original description of this species were relatively schematic, the aedeagus and modifications of the head in the male are illustrated.

  20. Evaluation of methyl methanesulfonate, 2,6-diaminotoluene and 5-fluorouracil: Part of the Japanese center for the validation of alternative methods (JaCVAM) international validation study of the in vivo rat alkaline comet assay.

    PubMed

    Plappert-Helbig, Ulla; Junker-Walker, Ursula; Martus, Hans-Joerg

    2015-07-01

    As a part of the Japanese Center for the Validation of Alternative Methods (JaCVAM)-initiative international validation study of the in vivo rat alkaline comet assay (comet assay), we examined methyl methanesulfonate, 2,6-diaminotoluene, and 5-fluorouracil under coded test conditions. Rats were treated orally with the maximum tolerated dose (MTD) and two additional descending doses of the respective compounds. In the MMS treated groups liver and stomach showed significantly elevated DNA damage at each dose level and a significant dose-response relationship. 2,6-diaminotoluene induced significantly elevated DNA damage in the liver at each dose and a statistically significant dose-response relationship whereas no DNA damage was obtained in the stomach. 5-fluorouracil did not induce DNA damage in either liver or stomach.

  1. Use of a standardized JaCVAM in vivo rat comet assay protocol to assess the genotoxicity of three coded test compounds; ampicillin trihydrate, 1,2-dimethylhydrazine dihydrochloride, and N-nitrosodimethylamine.

    PubMed

    McNamee, J P; Bellier, P V

    2015-07-01

    As part of the Japanese Center for the Validation of Alternative Methods (JaCVAM)-initiative international validation study of the in vivo rat alkaline comet assay (comet assay), our laboratory examined ampicillin trihydrate (AMP), 1,2-dimethylhydrazine dihydrochloride (DMH), and N-nitrosodimethylamine (NDA) using a standard comet assay validation protocol (v14.2) developed by the JaCVAM validation management team (VMT). Coded samples were received by our laboratory along with basic MSDS information. Solubility analysis and range-finding experiments of the coded test compounds were conducted for dose selection. Animal dosing schedules, the comet assay processing and analysis, and statistical analysis were conducted in accordance with the standard protocol. Based upon our blinded evaluation, AMP was not found to exhibit evidence of genotoxicity in either the rat liver or stomach. However, both NDA and DMH were observed to cause a significant increase in % tail DNA in the rat liver at all dose levels tested. While acute hepatoxicity was observed for these compounds in the high dose group, in the investigators opinion there were a sufficient number of consistently damaged/measurable cells at the medium and low dose groups to judge these compounds as genotoxic. There was no evidence of genotoxicity from either NDA or DMH in the rat stomach. In conclusion, our laboratory observed increased DNA damage from two blinded test compounds in rat liver (later identified as genotoxic carcinogens), while no evidence of genotoxicity was observed for the third blinded test compound (later identified as a non-genotoxic, non-carcinogen). This data supports the use of a standardized protocol of the in vivo comet assay as a cost-effective alternative genotoxicity assay for regulatory testing purposes.

  2. Evaluation of 4,4'-diaminodiphenyl ether in the rat comet assay: Part of the Japanese Center for the Validation of Alternative Methods (JaCVAM)-initiative international validation study of in vivo rat alkaline comet assay.

    PubMed

    Priestley, Catherine C; Walker, Joanne S; O'Donovan, Michael R; Doherty, Ann T

    2015-07-01

    As a part of the Japanese Center for the Validation of Alternative Methods (JaCVAM)-initiative international validation study of the in vivo rat alkaline comet assay, 4,4'-diaminodiphenyl ether (DPE), a known rodent genotoxic carcinogen, was tested in this laboratory. Sprague Dawley rats (7-9 weeks of age) were given three oral doses of DPE, 24 and 21 h apart and liver or stomach sampled 3h after the final dose. Under the conditions of the test, no increases in DNA damage in liver and stomach were observed with DPE (up to 200 mg/kg/day). A dose-dependent decrease in DNA migration, compared to vehicle controls, was noted for DPE in rat stomach. Further analysis is required to elucidate fully whether this decrease is a consequence of the mode of action or due to the toxicity of DPE. What is perhaps surprising is the inability of the comet assay to detect a known rat genotoxic carcinogen in liver. Further investigation is needed to clarify whether this apparent lack of response results from limited tissue exposure or metabolic differences between species. This finding highlights a need for careful consideration of study design when evaluating assay performance as a measure of in vivo genotoxicity.

  3. JaCVAM-organized international validation study of the in vivo rodent alkaline comet assay for the detection of genotoxic carcinogens: I. Summary of pre-validation study results.

    PubMed

    Uno, Yoshifumi; Kojima, Hajime; Omori, Takashi; Corvi, Raffaella; Honma, Masamistu; Schechtman, Leonard M; Tice, Raymond R; Burlinson, Brian; Escobar, Patricia A; Kraynak, Andrew R; Nakagawa, Yuzuki; Nakajima, Madoka; Pant, Kamala; Asano, Norihide; Lovell, David; Morita, Takeshi; Ohno, Yasuo; Hayashi, Makoto

    2015-07-01

    The in vivo rodent alkaline comet assay (comet assay) is used internationally to investigate the in vivo genotoxic potential of test chemicals. This assay, however, has not previously been formally validated. The Japanese Center for the Validation of Alternative Methods (JaCVAM), with the cooperation of the U.S. NTP Interagency Center for the Evaluation of Alternative Toxicological Methods (NICEATM)/the Interagency Coordinating Committee on the Validation of Alternative Methods (ICCVAM), the European Centre for the Validation of Alternative Methods (ECVAM), and the Japanese Environmental Mutagen Society/Mammalian Mutagenesis Study Group (JEMS/MMS), organized an international validation study to evaluate the reliability and relevance of the assay for identifying genotoxic carcinogens, using liver and stomach as target organs. The ultimate goal of this validation effort was to establish an Organisation for Economic Co-operation and Development (OECD) test guideline. The purpose of the pre-validation studies (i.e., Phase 1 through 3), conducted in four or five laboratories with extensive comet assay experience, was to optimize the protocol to be used during the definitive validation study.

  4. Ecology for the shrinking city (JA)

    EPA Science Inventory

    This article brings together the concepts of shrinking cities—the hundreds of cities worldwide experiencing long-term population loss—and ecology for the city. Ecology for the city is the application of a social–ecological understanding to shaping urban form and function along su...

  5. Personnel Management: A J/A Perspective

    ERIC Educational Resources Information Center

    Tasca, A. J.

    1974-01-01

    Recently, personnel executives and their staffs are being asked to help management solve an increasing number of human resource and business problems. Personnel management must undergo some changes if it is to achieve its full potential. (Author/AJ)

  6. 30 CFR 206.102 - How do I calculate royalty value for oil that I or my affiliate sell(s) under an arm's-length...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... must base value on the highest price the seller can receive through legally enforceable claims under... is entitled to, you must pay royalty at a value based upon that obtainable price or benefit. But you... value the oil under § 206.103. You may not otherwise use the price or differential specified in an...

  7. 30 CFR 206.52 - How do I calculate royalty value for oil that I or my affiliate sell(s) or exchange(s) under an...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ...) You must base value on the highest price that the seller can receive through legally enforceable... prices or benefits to which it is entitled, you must base value on that obtainable price or benefit. (1... value the oil using the NYMEX price, adjusted for applicable location and quality differentials...

  8. Evaluation of p-phenylenediamine, o-phenylphenol sodium salt, and 2,4-diaminotoluene in the rat comet assay as part of the Japanese Center for the Validation of Alternative Methods (JaCVAM)-initiated international validation study of in vivo rat alkaline comet assay.

    PubMed

    De Boeck, Marlies; van der Leede, Bas-jan; De Vlieger, Kathleen; Geys, Helena; Vynckier, An; Van Gompel, Jacky

    2015-07-01

    As part of the Japanese Center for the Validation of Alternative Methods (JaCVAM)-initiated international validation study of in vivo rat alkaline comet assay (comet assay), p-phenylenediamine dihydrochloride (PPD), o-phenylphenol sodium salt (OPP), and 2,4-diaminotoluene (2,4-DAT), were analyzed in this laboratory as coded test chemicals. Male Sprague-Dawley rats (7-9 weeks of age) were given three oral doses of the test compounds, 24 and 21 h apart and liver and stomach were sampled 3h after the final dose administration. Under the conditions of the test, no increases in DNA damage were observed in liver and stomach with PPD and OPP up to 100 and 1000 mg/kg/day, respectively. 2,4-DAT, a known genotoxic carcinogen, induced a weak but reproducible, dose-related and statistically significant increase in DNA damage in liver cells while no increases were observed in stomach cells.

  9. Selling Physics to Unwilling Buyers: Using Hollywood to Recruit for Physics

    NASA Astrophysics Data System (ADS)

    Krauss, Lawrence

    2003-03-01

    If confronting misconceptions is one way to learn, as many researchers have suggested, then Hollywood primes many young people to learn physics. At the same time, it is important to demonstrate to the public that the real world is actually more interesting than the world of science fiction. I will use examples from TV and movies, in particular Star Trek and the X-Files, to have some fun and to explore some interesting physics at the same time.

  10. 40 CFR 165.65 - Registrants who distribute or sell pesticide products in refillable containers.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... formula that is required under FIFRA section 3. (c) What information must I develop? For each pesticide... determined meets the standards in subpart C of this part and is compatible with the pesticide formulation... requirements in subpart C of this part. (d) What requirements must my individual establishments...

  11. Why and How the Tobacco Industry Sells Cigarettes to Young Adults: Evidence From Industry Documents

    PubMed Central

    Ling, Pamela M.; Glantz, Stanton A.

    2002-01-01

    Objectives. To improve tobacco control campaigns, we analyzed tobacco industry strategies that encourage young adults (aged 18 to 24) to smoke. Methods. Initial searches of tobacco industry documents with keywords (e.g., “young adult”) were extended by using names, locations, and dates. Results. Approximately 200 relevant documents were found. Transitions from experimentation to addiction, with adult levels of cigarette consumption, may take years. Tobacco marketing solidifies addiction among young adults. Cigarette advertisements encourage regular smoking and increased consumption by integrating smoking into activities and places where young adults' lives change (e.g., leaving home, college, jobs, the military, bars). Conclusions. Tobacco control efforts should include both adults and youths. Life changes are also opportunities to stop occasional smokers' progress to addiction. Clean air policies in workplaces, the military, bars, colleges, and homes can combat tobacco marketing. (Am J Public Health. 2002;92:908–916) PMID:12036776

  12. An overview of the synthetic routes to the best selling drugs containing 6-membered heterocycles

    PubMed Central

    2013-01-01

    Summary This review which is the second in this series summarises the most common synthetic routes as applied to the preparation of many modern pharmaceutical compounds categorised as containing a six-membered heterocyclic ring. The reported examples are based on the top retailing drug molecules combining synthetic information from both scientific journals and the wider patent literature. It is hoped that this compilation, in combination with the previously published review on five-membered rings, will form a comprehensive foundation and reference source for individuals interested in medicinal, synthetic and preparative chemistry. PMID:24204439

  13. Selling Environmental Products to the Government: Your Map to the Federal Marketplace

    EPA Pesticide Factsheets

    The purpose of this booklet is to provide you, the vendor, with information to help you enter the federal marketplace and thereby increase the availability of environmental productsto federal customers.

  14. Innovations: Alcohol & drug abuse: Narcotics on the net: the availability of Web sites selling controlled substances.

    PubMed

    Forman, Robert F

    2006-01-01

    The Internet is not only a vital medium for communication, entertainment, and commerce, but it is also an outlet for illicit drug sales. Although the U.S. Controlled Substances Act regulates access to certain drugs by requiring prescriptions, unique characteristics of the Internet create significant challenges for the enforcement of U.S. drug policies. In the late 1990s "no prescription Web sites" (NPWs) began to emerge, which allow persons to purchase drugs, such as opiates, without a prescription. Given the likely role of NPWs in increasing prescription drug abuse, health care professionals must develop and disseminate strategies for helping patients who are affected by these Web sites.

  15. Aggression and sexual behavior in best-selling pornography videos: a content analysis update.

    PubMed

    Bridges, Ana J; Wosnitzer, Robert; Scharrer, Erica; Sun, Chyng; Liberman, Rachael

    2010-10-01

    This current study analyzes the content of popular pornographic videos, with the objectives of updating depictions of aggression, degradation, and sexual practices and comparing the study's results to previous content analysis studies. Findings indicate high levels of aggression in pornography in both verbal and physical forms. Of the 304 scenes analyzed, 88.2% contained physical aggression, principally spanking, gagging, and slapping, while 48.7% of scenes contained verbal aggression, primarily name-calling. Perpetrators of aggression were usually male, whereas targets of aggression were overwhelmingly female. Targets most often showed pleasure or responded neutrally to the aggression.

  16. Frogs Sell Beer: The Effects of Beer Advertisements on Adolescent Drinking Knowledge, Attitudes, and Behavior.

    ERIC Educational Resources Information Center

    Gentile, Douglas A.; Walsh, David A.; Bloomgren, Barry W., Jr.; Atti, Jule A.; Norman, Jessica A.

    This present research reveals how beer advertising affects adolescents' knowledge of beer brands, drinking attitudes, and drinking behaviors. In addition to traditional psychological approaches for measuring media effects on alcohol-related behaviors and attitudes, market research advertising tracking methods were included to permit a clearer and…

  17. Sell a book and cook a dog: misery, memory and space from Siberian camps to Apartheid.

    PubMed

    van Dongen, Els

    2004-09-01

    In this article, the life story of a Polish woman in South Africa is used to illustrate that stories of exile are 'matter out of place' in host countries. Space is a central category in both the story of exiles and in the collective history of receiving countries, but it has different emotional, social, cultural and political meanings. Sometimes stories of exile are denied a place in the social memory of a society. Therefore, recollection and making sense of experienced misery in foreign contexts is often a difficult and lonely undertaking. The article links the story to macro-political processes and attempts to answer questions regarding the consequences of the lack of meaningful space for exiles to remember. Collective and national memories and ceremonies are often exclusive and offer no place for 'alien' memories. Therapeutic repertoires often fail to address the emotional value of communal sharing a socio-cultural and (meta) physical space. It is argued that because the state does not take responsibility for providing a (symbolic) space for the mourning and reconciliation of people whose memories are out of place, transcultural psychiatry - its subsystem - will have to reflect on how to provide such a space.

  18. Short-selling constraints as cause for price distortions: An experimental study

    PubMed Central

    Hauser, Florian; Huber, Jürgen

    2012-01-01

    In this paper we explore the influence of the possibility to short stocks and/or borrow money in laboratory markets. A key innovation of our study is that subjects can simultaneously trade two risky assets on two double-auction markets, allowing us to differentiate between assets with relatively high versus low capitalization. Divergence of opinions is created by providing each trader with noisy information on the intrinsic values of both assets. We find that when borrowing money or shorting stocks is restricted prices are systematically distorted. Specifically, stocks with high (low) capitalization are traded at lower (higher) prices than their fundamental value. Lifting the restrictions leads to more efficient prices and more liquidity, thereby also lowering volatility and bid-ask spreads. PMID:26523081

  19. Why do Institutions of Higher Education Reward Research While Selling Education? NBER Working Paper No. 14974

    ERIC Educational Resources Information Center

    Remler, Dahlia K.; Pema, Elda

    2009-01-01

    Higher education institutions and disciplines that traditionally did little research now reward faculty largely based on research, both funded and unfunded. Some worry that faculty devoting more time to research harms teaching and thus harms students' human capital accumulation. The economics literature has largely ignored the reasons for and…

  20. Characterisation of shops selling veterinary medicines in a tsetse-infested area of Kenya.

    PubMed

    Bett, B; Machila, N; Gathura, P B; McDermott, J J; Eisler, M C

    2004-04-30

    In a tsetse-infested area of Kenya, we characterised veterinary-drug outlets based on personnel and business characteristics to assess their capacities to provide clinical veterinary services. Structured questionnaires were administered to the retailers and sought information on the characteristics of the owners, salespersons and businesses. A total of 41 retail outlets (20 agro-veterinary, 11 pharmacy, and 10 general shops) were identified. There was poor response to questions on owner characteristics. Proprietors, who had no more than secondary education owned 15 out of 28 shops. Few shops (4/29) were owned by proprietors, who had professional qualifications (in animal health). Most salespersons had only secondary education but no qualifications. Animal-health assistants (AHAs), veterinarians and manufacturer's package inserts (drug leaflets) were the preferred information sources for the retailers. We concluded that drug retailers were poorly equipped with the technical knowledge necessary for drug dispensation and advice.

  1. 40 CFR 165.67 - Registrants who distribute or sell pesticide products to refillers for repackaging.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... pesticide products to refillers for repackaging. 165.67 Section 165.67 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) PESTICIDE PROGRAMS PESTICIDE MANAGEMENT AND DISPOSAL Standards for Repackaging Pesticide Products into Refillable Containers § 165.67 Registrants who distribute or...

  2. 40 CFR 165.67 - Registrants who distribute or sell pesticide products to refillers for repackaging.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... pesticide products to refillers for repackaging. 165.67 Section 165.67 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) PESTICIDE PROGRAMS PESTICIDE MANAGEMENT AND DISPOSAL Standards for Repackaging Pesticide Products into Refillable Containers § 165.67 Registrants who distribute or...

  3. 40 CFR 165.67 - Registrants who distribute or sell pesticide products to refillers for repackaging.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... pesticide products to refillers for repackaging. 165.67 Section 165.67 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) PESTICIDE PROGRAMS PESTICIDE MANAGEMENT AND DISPOSAL Standards for Repackaging Pesticide Products into Refillable Containers § 165.67 Registrants who distribute or...

  4. 40 CFR 165.67 - Registrants who distribute or sell pesticide products to refillers for repackaging.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... pesticide products to refillers for repackaging. 165.67 Section 165.67 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) PESTICIDE PROGRAMS PESTICIDE MANAGEMENT AND DISPOSAL Standards for Repackaging Pesticide Products into Refillable Containers § 165.67 Registrants who distribute or...

  5. 40 CFR 165.67 - Registrants who distribute or sell pesticide products to refillers for repackaging.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... pesticide products to refillers for repackaging. 165.67 Section 165.67 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) PESTICIDE PROGRAMS PESTICIDE MANAGEMENT AND DISPOSAL Standards for Repackaging Pesticide Products into Refillable Containers § 165.67 Registrants who distribute or...

  6. Mixed-mode sorption of hydroxylated atrazine degradation products to sell: A mechanism for bound residue

    USGS Publications Warehouse

    Lerch, R.N.; Thurman, E.M.; Kruger, E.L.

    1997-01-01

    This study tested the hypothesis that sorption of hydroxylated atrazine degradation products (HADPs: hydroxyatrazine, HA; deethylhydroxyatrazine, DEHA; and deisopropylhydroxyatrazine, DIHA) to soils occurs by mixed-mode binding resulting from two simultaneous mechanisms: (1) cation exchange and (2) hydrophobic interaction. The objective was to use liquid chromatography and soil extraction experiments to show that mixed-mode binding is the mechanism controlling HADP sorption to soils and is also a mechanism for bound residue. Overall, HADP binding to solid-phase extraction (SPE) sorbents occurred in the order: cation exchange >> octadecyl (C18) >> cyanopropyl. Binding to cation exchange SPE and to a high-performance liquid chromatograph octyl (C8) column showed evidence for mixed-mode binding. Comparison of soil extracted by 0.5 M KH2P04, pH 7.5, or 25% aqueous CH3CN showed that, for HA and DIHA, cation exchange was a more important binding mechanism to soils than hydrophobic interaction. Based on differences between several extractants, the extent of HADP mixed-mode binding to soil occurred in the following order: HA > DIHA > DEHA. Mixed-mode extraction recovered 42.8% of bound atrazine residues from aged soil, and 88% of this fraction was identified as HADPs. Thus, a significant portion of bound atrazine residues in soils is sorbed by the mixed-mode binding mechanisms.

  7. Magic truffles or Philosopher's stones: a legal way to sell psilocybin?

    PubMed

    Pellegrini, Manuela; Rotolo, Maria Concetta; Marchei, Emilia; Pacifici, Roberta; Saggio, Francesco; Pichini, Simona

    2013-03-01

    "Magic mushrooms" is the most common name given to hallucinogenic fungi containing the psychoactive alkaloids psilocybin and psilocin. In recent years, fungis' sclerotia, commonly called "magic truffles" have become a form of supply of psychoactive Psilocybe alkaloids since Psilocybe sclerotia are not specifically included in the laws banning the sale, the purchase and the use of such substances and mushrooms containing them. A liquid chromatography -tandem mass spectrometry (LC-MS/MS) method was developed for the rapid determination of psilocybin and psilocin in Psilocybe sclerotia. Following a simple step extraction with methanol, the alkaloids were separated on a reversed-phase column using a gradient of 0.1% formic acid - acetonitrile s a mobile phase at a flow rate of 0.2 mL/min.. Separated analytes were detected by electrospray ionization tandem mass spectrometry in the positive ion mode using multiple reaction monitoring. The developed method was linear over the calibration range for all two substances under investigation, with a r(2)  > 0.99. The detection and quantification limits were 0.3 µg and 1 µg per 100 mg truffles, for both psilocin and psilocybin and the intra- and inter-day coefficients of variation were always better than 15%. Using this method, the presence of only psilocybin was demonstrated in examined Psilocybe sclerotia. The content of psilocybin was found to vary over a concentration range of 59.3 to 167.8 µg per 100 mg of fresh sclerotia.

  8. "Re-Culturing" Students and Selling Futures: School-to-Work Policy in Ontario

    ERIC Educational Resources Information Center

    Taylor, Alison

    2005-01-01

    This paper situates recent school-to-work transition policy in Ontario, Canada, within the historical context of secondary school reform in the past 50 years. This understanding informs our analysis of interviews with representatives from government, business, organised labour, education and partnership brokers. Data suggest tensions between the…

  9. Packaging radium, selling science: boxes, bottles and other mundane things in the world of science.

    PubMed

    Rentetzi, Maria

    2011-07-01

    This article discusses the intersection of science and culture in the marketplace and explores the ways in which radium quack and medicinal products were packaged and labelled in the early twentieth century US. Although there is an interesting growing body of literature by art historians on package design, historians of science and medicine have paid little to no attention to the ways scientific and medical objects that were turned into commodities were packaged and commercialized. Thinking about packages not as mere containers but as multifunctional tools adds to historical accounts of science as a sociocultural enterprise and reminds us that science has always been part of consumer culture. This paper suggests that far from being receptacles that preserve their content and facilitate their transportation, bottles and boxes that contained radium products functioned as commercial and epistemic devices. It was the 1906 Pure Food and Drug Act that enforced such functions. Packages worked as commercial devices in the sense that they were used to boost sales. In addition, 'epistemic' points to the fact that the package is an artefact that ascribes meaning to and shapes its content while at the same time working as a device for distinguishing between patent and orthodox medicines.

  10. 18 CFR 367.9120 - Account 912, Demonstrating and selling expenses.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... expenses of merchandising, jobbing and contract work (§ 367.4160), or 930.1, General advertising expenses... appliances and equipment, prior to general public acceptance. (5) Solicitation of new customers or...

  11. The stem cell debate continues: the buying and selling of eggs for research.

    PubMed

    Baylis, F; McLeod, C

    2007-12-01

    Now that stem cell scientists are clamouring for human eggs for cloning-based stem cell research, there is vigorous debate about the ethics of paying women for their eggs. Generally speaking, some claim that women should be paid a fair wage for their reproductive labour or tissues, while others argue against the further commodification of reproductive labour or tissues and worry about voluntariness among potential egg providers. Siding mainly with those who believe that women should be financially compensated for providing eggs for research, the new stem cell guidelines of the International Society for Stem Cell Research (ISSCR) legitimise both reimbursement of direct expenses and financial compensation for many women who supply eggs for research. In this paper, the authors do not attempt to resolve the thorny issue of whether payment for eggs used in human embryonic stem cell research is ethically legitimate. Rather, they want to show specifically that the ISSCR recommended payment practices are deeply flawed and, more generally, that all payment schemes that aim to avoid undue inducement of women risk the global exploitation of economically disadvantaged women.

  12. ‘Acceptable rebellion’: marketing hipster aesthetics to sell Camel cigarettes in the US

    PubMed Central

    Hendlin, Yogi; Anderson, Stacey J; Glantz, Stanton A

    2011-01-01

    Objective The aim of the present work was to understand why and how RJ Reynolds and other tobacco companies have marketed tobacco products to young adult social trendsetting consumers (termed ‘hipsters’) to recruit trendsetters and average consumers to smoke. Methods Analysis of tobacco industry documents and industry marketing materials. Results Since 1995, RJ Reynolds developed its marketing campaigns to better suit the lifestyle, image identity and attitudes of hip trendsetters (so-called ‘hipsters’), and Camel’s brand identity actively shifted to more closely convey the hipster persona. Camel emphasised in-venue events such as promotional music tours to link the brand and smoking to activities and symbols appealing to hipsters and their emulating masses. Conclusions To reach this targeted and socially valuable trend-setting population, public health advocates must tap into hipster psychology and expose to the targeted community the tobacco company’s efforts to infiltrate the hipster community to turn hipsters into tobacco-using role models. PMID:20501494

  13. Selling science 2.0: What scientific projects receive crowdfunding online?

    PubMed

    Schäfer, Mike S; Metag, Julia; Feustle, Jessica; Herzog, Livia

    2016-09-19

    Crowdfunding has emerged as an additional source for financing research in recent years. The study at hand identifies and tests explanatory factors influencing the success of scientific crowdfunding projects by drawing on news value theory, the "reputation signaling" approach, and economic theories of online payment. A standardized content analysis of 371 projects on English- and German-language platforms reveals that each theory provides factors influencing crowdfunding success. It shows that projects presented on science-only crowdfunding platforms have a higher success rate. At the same time, projects are more likely to be successful if their presentation includes visualizations and humor, the lower their targeted funding is, the less personal data potential donors have to relinquish and the more interaction between researchers and donors is possible. This suggests that after donors decide to visit a scientific crowdfunding platform, factors unrelated to science matter more for subsequent funding decisions, raising questions about the potential and implications of crowdfunding science.

  14. Selling Indian Education: Fundraising and American Indian Identities at Bacone College, 1880-1941

    ERIC Educational Resources Information Center

    Neuman, Lisa K.

    2007-01-01

    Historically, American Indian education in the United States was inextricably linked to Euro-American colonialism. By the late nineteenth century, many Euro-Americans thought Native Americans were a "vanishing race," and schools for Indians incorporated this belief into their design. In the United States, the large number and variety of…

  15. When Sex Doesn't Sell: Using Sexualized Images of Women Reduces Support for Ethical Campaigns

    PubMed Central

    Bongiorno, Renata; Bain, Paul G.; Haslam, Nick

    2013-01-01

    Images of scantily clad women are used by advertisers to make products more attractive to men. This “sex sells” approach is increasingly employed to promote ethical causes, most prominently by the animal-rights organization PETA. Yet sexualized images can dehumanize women, leaving an unresolved paradox – is it effective to advertise an ethical cause using unethical means? In Study 1, a sample of Australian male undergraduates (N = 82) viewed PETA advertisements containing either sexualized or non-sexualized images of women. Intentions to support the ethical organization were reduced for those exposed to the sexualized advertising, and this was explained by their dehumanization of the sexualized women, and not by increased arousal. Study 2 used a mixed-gender community sample from the United States (N = 280), replicating this finding and extending it by showing that behaviors helpful to the ethical cause diminished after viewing the sexualized advertisements, which was again mediated by the dehumanization of the women depicted. Alternative explanations relating to the reduced credibility of the sexualized women and their objectification were not supported. When promoting ethical causes, organizations may benefit from using advertising strategies that do not dehumanize women. PMID:24367591

  16. American Indian Internet Cigarette Sales: Another Avenue for Selling Tobacco Products

    PubMed Central

    Hodge, Felicia S.; Geishirt Cantrell, Betty A.; Struthers, Roxanne; Casken, John

    2004-01-01

    A study conducted by the University of Minnesota found that cigarettes can be purchased on American Indian–owned Internet sites for about one fifth of the price at grocery stores, making this a more convenient, lower-priced, and appealing method of purchasing cigarettes. Researchers and educators are challenged to address this new marketing ploy and to discover ways to curb rising smoking rates in American Indian communities. PMID:14759938

  17. Selling Principles: Influencing Principles of Marketing Students' Perceptions of and Attitudes toward Marketing as a Discipline

    ERIC Educational Resources Information Center

    Camey, John P.; Williams, Janice K.

    2004-01-01

    Many marketing departments have experienced decreasing enrollments in marketing courses and difficulty recruiting students into the marketing major. This article examines and validates the Principles of Marketing class as significantly influencing students' overall perceptions of and attitudes toward marketing and the pursuit of marketing as their…

  18. Selling health lifestyles: using social marketing to promote change and prevent disease.

    PubMed

    Langill, Donna

    2004-11-01

    As part of its continuing mission to serve trustees and staff of health foundations and corporate giving programs, Grantmakers In Health (GIH) brought together grantmakers, researchers, and public health professionals on May 20, 2004 to discuss the application of social marketing principles to health promotion and chronic disease prevention. As a behavior change technique, social marketing has proven effective in motivating people to make the complex and difficult behavior changes that can improve health and reduce the risk of chronic diseases such as cardiovascular disease, cancer, and diabetes. The Issue Dialogue used the lens of tobacco prevention and cessation, physical activity, and healthy eating to examine how health grantmakers can use social marketing principles and techniques to encourage and support the adoption of healthier behaviors across the lifespan. This Issue Brief incorporates the information and ideas shared at the meeting with a background paper on social marketing that was prepared for participants who attended the Issue Dialogue. It starts with an introduction of social marketing concepts and provides a framework for assessing whether social marketing is an appropriate approach to use in addressing a particular issue. Subsequent sections: (1) describe both the social marketing communications process and techniques, using examples from campaigns developed by health grantmakers and others; (2) describe how social marketing can be used to promote policy change; (3) provide information on communication strategies that can complement social marketing; and (4) present opportunities for grantmakers.

  19. 18 CFR 367.9120 - Account 912, Demonstrating and selling expenses.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... ACT OF 2005, FEDERAL POWER ACT AND NATURAL GAS ACT UNIFORM SYSTEM OF ACCOUNTS FOR CENTRALIZED SERVICE... schools, preparing recipes, and related home service activities. (3) Exhibitions, displays, lectures,...

  20. Buying and Selling Words: What Every Good Librarian Should Know about the Dictionary Business.

    ERIC Educational Resources Information Center

    Kister, Ken

    1993-01-01

    Discusses features to consider when selecting dictionaries. Topics addressed include the publishing industry; the dictionary market; profits from dictionaries; pricing; competitive marketing tactics, including similar titles, claims to numbers of entries and numbers of definitions, and similar physical appearance; a trademark infringement case;…