Lead in rice: analysis of baseline lead levels in market and field collected rice grains.

PubMed

Norton, Gareth J; Williams, Paul N; Adomako, Eureka E; Price, Adam H; Zhu, Yongguan; Zhao, Fang-Jie; McGrath, Steve; Deacon, Claire M; Villada, Antia; Sommella, Alessia; Lu, Ying; Ming, Lei; De Silva, P Mangala C S; Brammer, Hugh; Dasgupta, Tapash; Islam, M Rafiqul; Meharg, Andrew A

2014-07-01

In a large scale survey of rice grains from markets (13 countries) and fields (6 countries), a total of 1578 rice grain samples were analysed for lead. From the market collected samples, only 0.6% of the samples exceeded the Chinese and EU limit of 0.2 μg g(-1) lead in rice (when excluding samples collected from known contaminated/mine impacted regions). When evaluating the rice grain samples against the Food and Drug Administration's (FDA) provisional total tolerable intake (PTTI) values for children and pregnant women, it was found that only people consuming large quantities of rice were at risk of exceeding the PTTI from rice alone. Furthermore, 6 field experiments were conducted to evaluate the proportion of the variation in lead concentration in rice grains due to genetics. A total of 4 of the 6 field experiments had significant differences between genotypes, but when the genotypes common across all six field sites were assessed, only 4% of the variation was explained by genotype, with 9.5% and 11% of the variation explained by the environment and genotype by environment interaction respectively. Further work is needed to identify the sources of lead contamination in rice, with detailed information obtained on the locations and environments where the rice is sampled, so that specific risk assessments can be performed. Copyright © 2014 Elsevier B.V. All rights reserved.

Rice Cluster I, an Important Group of Archaea Producing Methane in Rice Fields

NASA Astrophysics Data System (ADS)

Conrad, R.

2006-12-01

Rice fields are an important source for the greenhouse gas methane. Methane is a major degradation product of organic matter in the anoxic soil, is partially oxidized in the rhizosphere and is emitted into the atmosphere through the aerenchyma system of the plants. Anaerobic degradation of organic matter by fermenting bacteria eventually results in the production of acetate and hydrogen, the two major substrates for microbial methanogenesis. The community of methanogenic archaea consists of several major orders or families including hydrogen-utilizing Rice Cluster-I (RC-I). Environmental conditions affect the methanogenic degradation process and the community structure of the methanogenic archaea in soil and rhizosphere. For example, populations of acetoclastic Methanosaetaceae and Methanosarcinaceae are enhanced by low and high acetate concentrations, respectively. Stable isotope probing of 16S rRNA showed that RC-I methanogens are mainly active on rice roots and at low H2 concentrations. Growth and population size is largely consistent with energetic conditions. RC-I methanogens on roots seem to be responsible for methane production from plant photosynthates that account for a major part of the emitted methane. Populations of RC-I methanogens in rice field soil are also enhanced at elevated temperatures (40-50°C). Moderately thermophilic members of RC-I methanogens or other methanogenic families were found to be ubiquitously present in soils from rice fields and river marshes. The genome of a RC-I methanogen was completely sequenced out of an enrichment culture using a metagenome approach. Genes found are consistent with life in the rhizosphere and in temporarily drained, oxic soil. We found that the methanogenic community structure on the rice roots is mainly determined by the respective community structure of the soil, but is in addition affected by the rice cultivar. Rice microcosms in which soil and rice roots are mainly colonized by RC-I methanogens produce

Gene flow from transgenic rice to red rice (Oryza sativa L.) in the field.

PubMed

Busconi, M; Baldi, G; Lorenzoni, C; Fogher, C

2014-01-01

In this study, we simulate a transgenic rice crop highly infested with red rice to examine transgene transfer from a transgenic line (A2504) resistant to glufosinate ammonium to cohabitant red rice. The red rice was sown along with the transgenic line at the highest density found in naturally infested crops in the region. Agricultural practices similar to those used to control red rice infestation in northern Italy rice fields were used to reproduce the local rice production system. During the first 2 years, the field was treated with herbicide at the appropriate time; in the first year the dosage of herbicide was three times the recommended amount. In this first year, detectable red rice plants that escaped herbicide treatment were manually removed. Nevertheless, two herbicide-resistant hybrid plants (named 101 and 104) were identified in the experimental field during the second year of cultivation. Phenotypic and molecular characterisation suggests the hybrid nature of these two plants, deriving from crossing events involving A2504, respectively, with red rice (plant 101) and the buffer cultivar Gladio (plant 104). The progeny of two subsequent generations of the two plants were examined and the presence of the transgene detected, indicating stable transfer of the transgene across generations. In conclusion, despite control methods, red rice progeny tolerant to the herbicide can be expected following use of transgenic rice and, consequently, difficulties in controlling this weed with chemicals will emerge in a relatively short time. © 2013 German Botanical Society and The Royal Botanical Society of the Netherlands.

Mosquitoes and other aquatic insects in fallow field biotopes and rice paddy fields.

PubMed

Ohba, S Y; Matsuo, T; Takagi, M

2013-03-01

Fallow field biotopes that develop from abandoned rice fields are man-made wetlands that provide new habitats for various aquatic animals. Although consideration of such biotopes generally focuses on their positive aspects, this study evaluated the negative aspects of establishing fallow field biotopes with regard to mosquito breeding sites. To determine whether fallow field biotopes become breeding habitats for vector mosquitoes, we evaluated mosquito fauna in fallow field biotopes and adjacent rice fields. We found larvae of Anopheles lesteri, Anopheles sinensis and Culex tritaeniorhynchus (all: Diptera: Culicidae) in the biotopes. Although abundances of mosquito larvae in the biotopes and rice fields were statistically similar, mosquito abundances in rice fields increased dramatically in August when the water level reduced after the rainy season. The abundance and variety of the mosquitoes' natural predators were greater in biotopes than in rice fields because the former are a permanent and stable aquatic environment. A generalized linear mixed model showed a negative effect of predator diversity on mosquito larvae abundance in both habitats. Although fallow field biotopes become breeding habitats for vector mosquitoes, establishing biotopes from fallow fields in order to protect various aquatic animals, including mosquito insect predators, may help to control mosquito breeding. © 2012 The Royal Entomological Society.

Diversity of Cultivable Methane-Oxidizing Bacteria in Microsites of a Rice Paddy Field: Investigation by Cultivation Method and Fluorescence in situ Hybridization (FISH)

PubMed Central

Dianou, Dayéri; Ueno, Chihoko; Ogiso, Takuya; Kimura, Makoto; Asakawa, Susumu

2012-01-01

The diversity of cultivable methane-oxidizing bacteria (MOB) in the rice paddy field ecosystem was investigated by combined culture-dependent and fluorescence in situ hybridization (FISH) techniques. Seven microsites of a Japanese rice paddy field were the focus of the study: floodwater, surface soil, bulk soil, rhizosphere soil, root, basal stem of rice plant, and rice stumps of previous harvest. Based on pmoA gene analysis and transmission electron microscopy (TEM), four type I, and nine type II MOB isolates were obtained from the highest dilution series of enrichment cultures. The type I MOB isolates included a novel species in the genus Methylomonas from floodwater and this is the first type I MOB strain isolated from floodwater of a rice paddy field. In the type I MOB, two isolates from stumps were closely related to Methylomonas spp.; one isolate obtained from rhizosphere soil was most related to Methyloccocus-Methylocaldum-Methylogaea clade. Almost all the type II MOB isolates were related to Methylocystis methanotrophs. FISH confirmed the presence of both types I and II MOB in all the microsites and in the related enrichment cultures. The study reported, for the first time, the diversity of cultivable methanotrophs including a novel species of type I MOB in rice paddy field compartments. Refining growth media and culture conditions, in combination with molecular approaches, will allow us to broaden our knowledge on the MOB community in the rice paddy field ecosystem and consequently to implement strategies for mitigating CH4 emission from this ecosystem. PMID:22446309

Modulation of DNA methylation machineries in japanese rice fish (Oryzias latipes) embryogenesis by ethanol and 5-azacytidine

USDA-ARS?s Scientific Manuscript database

As a sequel of our investigations on the impact of epigenome in inducing fetal alcohol spectrum disorder (FASD) phenotypes in Japanese rice fish, we investigated on several DNA methylation machinery genes including DNA methyl transferase 3ba (dnmt3ba) and methyl binding proteins (MBPs), namely, mbdl...

Identification of pyroglutamyl peptides in Japanese rice wine (sake): presence of hepatoprotective pyroGlu-Leu.

PubMed

Kiyono, Tamami; Hirooka, Kiyoo; Yamamoto, Yoshihiro; Kuniishi, Sunao; Ohtsuka, Maho; Kimura, Shikou; Park, Eun Young; Nakamura, Yasushi; Sato, Kenji

2013-11-27

Japanese rice wine, sake, is made from steamed rice, water, and lactic acid by "multiple parallel fermentation" with mold (Aspergillus oryzae) and yeast (Saccharomyces cerevisiae). Nineteen pyroglutamyl peptides were identified in commercially available sake. Among them, pyroGlu-Leu and pyroGlu-Gln were the major constituents. PyroGlu-Leu has been demonstrated to attenuate hepatitis and colitis in animal models. Commercial products (n = 5) contained pyroGlu-Leu at concentrations ranging from 40 to 60 μM (10-15 mg/L). The pyroGlu-Leu content in sake mash increased during the fermentation processes. However, no pyroGlu-Leu was produced by yeast inoculated into preheated mash. Furthermore, addition of (13)C-Leu to the mash did not increase the ratio of pyroGlu-(13)C-Leu to pyroGlu-(12)C-Leu. On the other hand, digestion of steamed rice with A. oryzae proteases increased the pyroGlu-Leu content. These results indicate that pyroGlu-Leu in sake is produced from rice proteins by digestion with A. oryzae proteases.

Avian foods, foraging and habitat conservation in world rice fields

USGS Publications Warehouse

Stafford, J.D.; Kaminski, R.M.; Reinecke, K.J.

2010-01-01

Worldwide, rice (Oryza sativa) agriculture typically involves seasonal flooding and soil tillage, which provides a variety of microhabitats and potential food for birds. Water management in rice fields creates conditions ranging from saturated mud flats to shallow (<30 cm) water, thereby attracting different guilds of birds. Grain not collected during harvest (i.e. waste rice) is typically the most abundant potential food of birds in rice fields, with estimates of seed mass from North America ranging from 66672 kg/ha. Although initially abundant after harvest, waste rice availability can be temporally limited. Few abundance estimates for other foods, such as vertebrate prey or forage vegetation, exist for rice fields. Outside North America, Europe and Japan, little is known about abundance and importance of any avian food in rice fields. Currently, flooding rice fields after harvest is the best known management practice to attract and benefit birds. Studies from North America indicate specific agricultural practices (e.g. burning stubble) may increase use and improve access to food resources. Evaluating and implementing management practices that are ecologically sustainable, increase food for birds and are agronomically beneficial should be global priorities to integrate rice production and avian conservation. Finally, land area devoted to rice agriculture appears to be stable in the USA, declining in China, and largely unquantified in many regions. Monitoring trends in riceland area may provide information to guide avian conservation planning in rice-agriculture ecosystems.

Effects of landscape features on waterbird use of rice fields

USGS Publications Warehouse

King, S.; Elphick, C.S.; Guadagnin, D.; Taft, O.; Amano, T.

2010-01-01

Literature is reviewed to determine the effects of landscape features on waterbird use of fields in regions where rice (Oryza sativa) is grown. Rice-growing landscapes often consist of diverse land uses and land cover, including rice fields, irrigation ditches, other agricultural fields, grasslands, forests and natural wetlands. Numerous studies indicate that local management practices, such as water depth and timing of flooding and drawdown, can strongly influence waterbird use of a given rice field. However, the effects of size and distribution of rice fields and associated habitats at a landscape scale have received less attention. Even fewer studies have focused on local and landscape effects simultaneously. Habitat connectivity, area of rice, distance to natural wetlands, and presence and distance to unsuitable habitat can be important parameters influencing bird use of rice fields. However, responses to a given landscape vary with landscape structure, scale of analysis, among taxa and within taxa among seasons. A lack of multi-scale studies, particularly those extending beyond simple presence and abundance of a given species, and a lack of direct tests comparing the relative importance of landscape features with in-field management activities limits understanding of the importance of landscape in these systems and hampers waterbird conservation and management.

Spectral and spatial characterization of rice field mosquito habitat

NASA Technical Reports Server (NTRS)

Wood, Byron L.; Beck, Louisa R.; Washino, Robert K.; Palchick, Susan M.; Sebesta, Paul D.

1991-01-01

Irrigated rice provides an ideal breeding habitat for Anopheles free-borni, the western malaria mosquito, throughout California. In a 1985 study, it was determined that early-season rice canopy development, as monitored using remotely sensed data, could be used to distinguish between high and low mosquito producing rice fields. This distinction could be made over two months prior to peak mosquito production. It was found that high-producing fields were located in an area characterized by a diversity of land use, including livestock pastures, whereas the low-producing fields were in an area devoted almost exclusively to the cultivation of rice. The ability to distinguish between high and low mosquito producing fields prior to peak mosquito production is important in terms of mosquito habitat surveillance and control.

Photodegradation of clothianidin under simulated California rice field conditions.

PubMed

Mulligan, Rebecca A; Redman, Zachary C; Keener, Megan R; Ball, David B; Tjeerdema, Ronald S

2016-07-01

Photodegradation can be a major route of dissipation for pesticides applied to shallow rice field water, leading to diminished persistence and reducing the risk of offsite transport. The objective of this study was to characterize the aqueous-phase photodegradation of clothianidin under simulated California rice field conditions. Photodegradation of clothianidin was characterized in deionized, Sacramento River and rice field water samples. Pseudo-first-order rate constants and DT50 values in rice field water (mean k = 0.0158 min(-1) ; mean DT50 = 18.0 equivalent days) were significantly slower than in deionized water (k = 0.0167 min(-1) ; DT50 = 14.7 equivalent days) and river water (k = 0.0146 min(-1) ; DT50 = 16.6 equivalent days) samples. Quantum yield ϕc values demonstrate that approximately 1 and 0.5% of the light energy absorbed results in photochemical transformation in pure and field water respectively. Concentrations of the photodegradation product thiazolymethylurea in aqueous photolysis samples were determined using liquid chromatography-tandem mass spectrometry and accounted for ≤17% in deionized water and ≤8% in natural water. Photodegradation rates of clothianidin in flooded rice fields will be controlled by turbidity and light attenuation. Aqueous-phase photodegradation may reduce the risk of offsite transport of clothianidin from flooded rice fields (via drainage) and mitigate exposure to non-target organisms. © 2015 Society of Chemical Industry. © 2015 Society of Chemical Industry.

The land use potential of flood-prone rice fields using floating rice system in Bojonegoro regency in East Java

NASA Astrophysics Data System (ADS)

Irianto, H.; Mujiyo; Riptanti, E. W.; Qonita, A.

2018-03-01

Bojonegoro regency occupies the largest flood-prone rice fields of about 14,198 hectares, in East Java province. Floods commonly occur due to Bengawan Solo river over-burst, particularly in rainy season. The fields are potential for cultivating rice, but floods lasting for months causing these areas to be unproductive. The objective of this article is to examine the potential land use of flood prone rice fields in Bojonegoro regency using floating rice system as an effort to maintain productivity in rainy season. The method of this study is referential study about the rice production using floating cultivation system in other regions, which are later compared with the physical condition of the fields in Bojonegoro. The results of analysis show that rice cultivation using floating system can maintain rice production in flood prone areas during rainy season. The potential production of rice is 5-6 tons/ha. However, technical problems for cultivating rice cannot be ignored since farmers are not familiar with cultivating flooded fields. This article also explains alternatives of floating rice cultivation technique, which can be implemented effectively and efficiently. Pioneer work of developing floating rice in Bojonegoro that has been done by the Team of Faculty of Agriculture of UNS, Surakarta, is expected to serve as a medium for accelerating the adoption of cultivation technology innovation to farmers.

Developmental regulation of neuroligin genes in Japanese rice fish (oryzias latipes) embryogenesis maintains the rhythym during ethanol-in

USDA-ARS?s Scientific Manuscript database

Although prenatal alcohol exposure is the potential cause of fetal alcohol spectrum disorder (FASD) in humans, the molecular mechanism(s) of FASD is yet unknown. We have used Japanese rice fish (Oryzias latipes) embryogenesis as an animal model of FASD and reported that this model has effectively ge...

Waterbird communities in rice fields subjected to different post-harvest treatments

USGS Publications Warehouse

Day, J.H.; Colwell, M.A.

1998-01-01

In California's Sacramento Valley, the potential value of rice fields as habitat for waterbirds may vary with harvest method, post-harvest treatment of rice straw (chopped, burned, plowed), and extent of flooding. Recent changes in rice harvesting methods (i.e., use of stripper-headers) and a legislative mandate to decrease burning of rice straw after harvest may alter habitat availability and use. Thus, we investigated species richness and community composition of nonbreeding waterbirds during October-March 1993-94 and 1994-95 in rice fields of the northern Sacramento Valley. Most (85-91% of land area) rice was conventionally harvested (i.e., cutter bar), and the remainder was stripped. Rice straw was left untreated in more than half of fields (52% in 1994 and 54% in 1995), especially in stripped fields (56-70%). In fields where farmers treated straw, the most common management methods were plowing (15-21%), burning (19-24%), and chopping (3-5%). Fields became increasingly wet from October through March as seasonal precipitation accumulated and farmers flooded fields to facilitate straw decomposition and provide habitat for ducks. Species richness of waterbirds was greater (P 0.23). Species richness in stripped fields probably was low because foraging opportunities were limited by tall dense straw, decreased grain density, and infrequent flooding. We recommend that land managers wishing to provide habitat for a diverse waterbird community harvest rice using conventional methods and flood fields shallowly.

Soil Respiration Controls Ionic Nutrient Concentration In Percolating Water In Rice Fields

NASA Astrophysics Data System (ADS)

Kimura, M.

2004-12-01

leached from the plow layer by percolating water amounted to 170 kgC ha-1 in a Japanese rice field, among which 120 kgC of organic materials were adsorbed in the subsoil layer between 13 and 40 cm depth.

Estimating high mosquito-producing rice fields using spectral and spatial data

NASA Technical Reports Server (NTRS)

Wood, B. L.; Beck, L. R.; Washino, R. K.; Hibbard, K. A.; Salute, J. S.

1992-01-01

The cultivation of irrigated rice provides ideal larval habitat for a number of anopheline vectors of malaria throughout the world. Anopheles freeborni, a potential vector of human malaria, is associated with the nearly 240,000 hectares of irrigated rice grown annually in Northern and Central California; therefore, this species can serve as a model for the study of rice field anopheline population dynamics. Analysis of field data revealed that rice fields with early season canopy development, that are located near bloodmeal sources (i.e., pastures with livestock) were more likely to produce anopheline larvae than fields with less developed canopies located further from pastures. Remote sensing reflectance measurements of early-season canopy development and geographic information system (GIS) measurements of distanes between rice fields and pastures with livestock were combined to distinguish between high and low mosquito-producing rice fields. Using spectral and distance measures in either a discriminant or Bayesian analysis, the identification of high mosquito-producing fields was made with 85 percent accuracy nearly two months before anopheline larval populations peaked. Since omission errors were also minimized by these approaches, they could provide a new basis for directing abatement techniques for the control of malaria vectors.

DNA methyltransferase expressions in Japanese rice fish (Oryzias latipes) embryogenesis is developmentally regulated and modulated by ethanol and 5-azacytidine

USDA-ARS?s Scientific Manuscript database

We aimed to investigate the impact of the epigenome in inducting fetal alcohol spectrum disorder (FASD) phenotypes in Japanese rice fish embryogenesis. One of the significant events in epigenome is DNA methylation which is catalyzed by DNA methyl transferase (DNMT) enzymes. We analyzed DNMT enzyme m...

Waterbird nest density and nest survival in rice fields of southwestern Louisiana

USGS Publications Warehouse

Pierluissi, S.; King, Sammy L.; Kaller, Michael D.

2010-01-01

Rice fields in southwestern Louisiana provide breeding habitat for several waterbird species; however, little is known about nest density, nest survival and the importance of landscape context of rice fields in determining breeding activity. In 2004, 42 rice fields were searched for nests, and 40 were searched in 2005. Land uses surrounding rice fields, including irrigation canals, trees, crawfish ponds, rice, fallow and soybean fields, were examined to determine influence on nest density and survival. Nest densities were 13.5-16.0 nests/km2 for Purple Gallinules (Porphyrio martinica), 3.0-13.7 nests/km2 for Fulvous Whistling Ducks (Dendrocygna bicolor), 2.6-2.8 nests/km2 for Common Moorhens (Gallinula chloropus), 0.3-0.92 nests/km2 for Least Bitterns (Ixobrychus exilisi) and 0-0.6 nests/km2 for Mottled Ducks (Anas fulvigula). Nest survival was 52-79% for Purple Gallinules and 39-43% for Fulvous Whistling Ducks. Apparent nest success of Common Moorhens was 73-75%, 83% for Least Bitterns and 33% for Mottled Ducks. Purple Gallinule and Common Moorhen nest densities were highest in fields with a larger proportion of irrigation canals surrounding rice fields. Purple Gallinule nest densities were greater in fields devoid of trees and landscapes dominated by rice fields and pasture, rather than landscapes containing soybean fields and residential areas. Fulvous Whistling Duck nest densities were higher in agriculturally-dominated landscapes with few trees.

Rapid assessment of rice seed availability for wildlife in harvested fields

USGS Publications Warehouse

Halstead, B.J.; Miller, M.R.; Casazza, Michael L.; Coates, P.S.; Farinha, M.A.; Benjamin, Gustafson K.; Yee, J.L.; Fleskes, J.P.

2011-01-01

Rice seed remaining in commercial fields after harvest (waste rice) is a critical food resource for wintering waterfowl in rice-growing regions of North America. Accurate and precise estimates of the seed mass density of waste rice are essential for planning waterfowl wintering habitat extents and management. In the Sacramento Valley of California, USA, the existing method for obtaining estimates of availability of waste rice in harvested fields produces relatively precise estimates, but the labor-, time-, and machineryintensive process is not practical for routine assessments needed to examine long-term trends in waste rice availability. We tested several experimental methods designed to rapidly derive estimates that would not be burdened with disadvantages of the existing method. We first conducted a simulation study of the efficiency of each method and then conducted field tests. For each approach, methods did not vary in root mean squared error, although some methods did exhibit bias for both simulations and field tests. Methods also varied substantially in the time to conduct each sample and in the number of samples required to detect a standard trend. Overall, modified line-intercept methods performed well for estimating the density of rice seeds. Waste rice in the straw, although not measured directly, can be accounted for by a positive relationship with density of rice on the ground. Rapid assessment of food availability is a useful tool to help waterfowl managers establish and implement wetland restoration and agricultural habitat-enhancement goals for wintering waterfowl. ?? 2011 The Wildlife Society.

Rice available to waterfowl in harvested fields in the Sacramento Valley, California

USGS Publications Warehouse

Miller, M.R.; Sharp, D.E.; Gilmer, D.S.; Mulvaney, W.R.

1989-01-01

Rice fields in the Sacramento Valley, California were sampled in 1985 and 1986 to determine the weight of rice seed remaining in the fields immediately after harvest and again after the fields were burned. No significant differences were found between years (P>0.05). The pooled mean was 388 kg/ha in harvested fields and 276 kg/ha in burned fields. These values are less than estimates previously available. The values for harvested fields both years were no different (P>0.05) than values obtained by the U.S. Department of Agriculture (USDA). Surveys of rice fields in December both years showed that most fields were left either harvested (26-32%) or burned (37-40%) through the winter. Fields flooded for duck hunting made up 15% of the total. The proportion of fields plowed by December increased from 14% in 1985 to 22% in 1986. Sixty-three percent of all fields that had been flooded for hunting were drained within two weeks after the end of the hunting season. Harvest yield field size levee type (contour, lasered), straw status (spread, windrowed), harvest date, and rice variety did not affect the quantity of seeds remaining after harvest (P>0.05). One harvester model, the Hardy Harvester, left more rice in fields than did others we tested (P<0.001). Specific management programs are recommended to mitigate annual variation in rice seed availability to waterfowl caused by differences in total hectares grown (15% less in 1986) and in the proportion of fields burned and plowed.

Taking transgenic rice drought screening to the field.

PubMed

Gaudin, Amélie C M; Henry, Amelia; Sparks, Adam H; Slamet-Loedin, Inez H

2013-01-01

Numerous transgenes have been reported to increase rice drought resistance, mostly in small-scale experiments under vegetative-stage drought stress, but few studies have included grain yield or field evaluations. Different definitions of drought resistance are currently in use for field-based and laboratory evaluations of transgenics, the former emphasizing plant responses that may not be linked to yield under drought. Although those fundamental studies use efficient protocols to uncover and validate gene functions, screening conditions differ greatly from field drought environments where the onset of drought stress symptoms is slow (2-3 weeks). Simplified screening methods, including severely stressed survival studies, are therefore not likely to identify transgenic events with better yield performance under drought in the target environment. As biosafety regulations are becoming established to allow field trials in some rice-producing countries, there is a need to develop relevant screening procedures that scale from preliminary event selection to greenhouse and field trials. Multilocation testing in a range of drought environments may reveal that different transgenes are necessary for different types of drought-prone field conditions. We describe here a pipeline to improve the selection efficiency and reproducibility of results across drought treatments and test the potential of transgenic rice for the development of drought-resistant material for agricultural purposes.

Investigating options for attenuating methane emission from Indian rice fields.

PubMed

Singh, S N; Verma, Amitosh; Tyagi, Larisha

2003-08-01

The development of methods and strategies to reduce the emission of methane from paddy fields is a central component of ongoing efforts to protect the Earth's atmosphere and to avert a possible climate change. It appears from this investigation that there can be more than one strategy to contain methane emission from paddy fields, which are thought to be a major source of methane emission in tropical Asia. Promising among the mitigating options may be water management, organic amendments, fertilizer application and selection of rice cultivars. It is always better to adopt multi-pronged strategies to contain CH4 efflux from rice wetlands. Use of fermented manures with low C/N ratio, application of sulfate-containing chemical fertilizers, selection of low CH4 emitting rice cultivars, and implementation of one or two short aeration periods before the heading stage can be effective options to minimize CH4 emission from paddy fields. Among these strategies, water management, which appears to be the best cost-effective and eco-friendly way for methane mitigation, is only possible when excess water is available for reflooding after short soil drying at the right timing and stage. However, in tropical Asia, rice fields are naturally flooded during the monsoonal rainy season and fully controlled drainage is often impossible. In such situation, water deficits during the vegetative and reproductive stage may drastically affect the rice yields. Thus, care must be taken to mitigate methane emission without affecting rice yields.

Bacterial DNA Detected in Japanese Rice Wines and the Fermentation Starters.

PubMed

Terasaki, Momoka; Fukuyama, Akari; Takahashi, Yurika; Yamada, Masato; Nishida, Hiromi

2017-12-01

As Japanese rice wine (sake) brewing is not done aseptically, bacterial contamination is conceivable during the process of sake production. There are two types of the fermentation starter, sokujo-moto and yamahai-moto (kimoto). We identified bacterial DNA found in various sakes, the sokujo-moto and the yamahai-moto making just after sake yeast addition. Each sake has a unique variety of bacterial DNA not observed in other sakes. Although most bacterial DNA sequences detected in the sokujo-moto were found in sakes of different sake breweries, most bacterial DNA sequences detected in the yamahai-moto at the early stage of the starter fermentation were not detected in any sakes. Our findings demonstrate that various bacteria grow and then die during the process of sake brewing, as indicated by the presence of trace levels of bacterial DNA.

[Ecological characteristics of phytoplankton in waters of biological-controlling and ordinary rice fields].

PubMed

Liu, W; Wang, Y; Xu, R

2001-02-01

Through investigations from March to June 1998 on the phytoplankton in waters of biological-controlled and ordinary rice fields, 112 species of phytoplankton were found, of which, Bacillariophyta, Euglenophyta and Chlorophyta were dominant. The comparison of the species in two rice fields showed that in biological-controlled rice field, there were more species of Euglenophyta, with 5 most dominant species, i.e., 2 of Euglenophyta, 2 of Bacillariophyta and 1 of Chlorophyta. In ordinary rice field, there were more Bacillariophyta species, in which, 5 most dominant species belonged to Bacillariophyta, except Scenedesmus bijuga to Chlorophyta. The biodiversities of phytoplankton and their evennesses were also analyzed with the period from the seedlings being planted to the rice fields being dried, showing that a little increase in their biodiversity mainly caused by the increase of species number and an evident decrease in their evennesses.

Remotely Sensing Larval Population Dynamics of Rice Field Anophelines

NASA Technical Reports Server (NTRS)

Beck, Louisa R.; Dister, Sheri W.; Wood, Byron L.; Washino, Robert K.

1997-01-01

The primary objective of both studies was to determine if RS and GIS techniques could be used to distinguish between high and low larval-producing rice fields in California. Results of the first study suggested that early-season green-up and proximity to livestock pastures were positively correlated with high larval abundance. Based on the early-season spectral differences between high and low larval-producing fields, it appeared that canopy development and tillering influenced mosquito habitat quality. At that time, rice fields consisted of a mixture of plants and water, a combination that allowed An. freeborni females to lay eggs in partial sunlight, protected from both predators and wind. This established a population earlier in the season than in other, 'less-green' fields where tillering and plant emergence was too minimal for ovipositioning. The study also indicated the importance of the distance that a mosquito would have to fly in order to take a bloodmeal prior to ovipositing. These associations were fully explored in an expanded study two years later. The second study confirmed the positive relationship between early season canopy development and larval abundance, and also demonstrated the relationship between abundance and distance-to-pasture. The association between greenness (as measured using NDVI), distance-to-pasture, and abundance is illustrated. The second study also indicated the siginificance of the landscape context of rice fields for larval production. Fields that included opportunities for feeding and resting within the flight range of the mosquito had higher abundances than did fields that were in a homogeneous rice area.

Rice Field Geochemistry and Hydrology: An Explanation for Why Groundwater Irrigated Fields in Bangladesh are Net Sinks of Arsenic from Groundwater

PubMed Central

Neumann, Rebecca B.; St. Vincent, Allison P.; Roberts, Linda C.; Badruzzaman, A. Borhan M.; Ali, M. Ashraf; Harvey, Charles F.

2011-01-01

Irrigation of rice fields in Bangladesh with arsenic-contaminated groundwater transfers tens of cubic kilometers of water and thousands of tons of arsenic from aquifers to rice fields each year. Here we combine observations of infiltration patterns with measurements of porewater chemical composition from our field site in Munshiganj Bangladesh to characterize the mobility of arsenic in soils beneath rice fields. We find that very little arsenic delivered by irrigation returns to the aquifer, and that recharging water mobilizes little, if any, arsenic from rice field subsoils. Arsenic from irrigation water is deposited on surface soils and sequestered along flow paths that pass through bunds, the raised soil boundaries around fields. Additionally, timing of flow into bunds limits the transport of biologically available organic carbon from rice fields into the subsurface where it could stimulate reduction processes that mobilize arsenic from soils and sediments. Together, these results explain why groundwater irrigated rice fields act as net sinks of arsenic from groundwater. PMID:21332196

A comparison of weed communities of coastal rice fields in Peninsular Malaysia.

PubMed

Hakim, M A; Juraimi, Abdul Shukor; Hanafi, M M; Selamat, A

2013-09-01

A survey was conducted at 100 different rice fields in coastal areas of West Malaysia to identify most common and prevalent weeds associated with rice. Fields surveyed were done according to the quantitative survey method by using 0.5m x 0.5m size quadrate with 20 samples from each field. A total of 53 different weed species belong to 18 families were identified of which 32 annual and 21 perennial; 12 grassy, 13 sedges and 28 broadleaved weeds. Based on relative abundance the most prevalent and abundant weed species were selected in the coastal rice field. Among the 10 most abundant weed species, there were four grasses viz. Echinochloa crusgalli, Leptochloo chinensis, Echinochloo colona, Oryza sotivo L. (weedy rice).; four sedges viz. Fimbristylis miliacea, Cyperus iria, Cyperus difformis, Scirpus grossus and two broadleaved weeds viz. Sphenocleo zeylonica, Jussiaea linifolio. Leptochloa chinensis, E. crusgalli, F. miliocea, E. colona were more prevalent and abundant species out of the 10 most dominant weed species in the coastal rice field of Peninsular Malaysia.

Performance of hybrids between weedy rice and insect-resistant transgenic rice under field experiments: implication for environmental biosafety assessment.

PubMed

Cao, Qian-Jin; Xia, Hui; Yang, Xiao; Lu, Bao-Rong

2009-12-01

Transgene escape from genetically modified (GM) rice into weedy rice via gene flow may cause undesired environmental consequences. Estimating the field performance of crop-weed hybrids will facilitate our understanding of potential introgression of crop genes (including transgenes) into weedy rice populations, allowing for effective biosafety assessment. Comparative studies of three weedy rice strains and their hybrids with two GM rice lines containing different insect-resistance transgenes (CpTI or Bt/CpTI) indicated an enhanced relative performance of the crop-weed hybrids, with taller plants, more tillers, panicles, and spikelets per plant, as well as higher 1 000-seed weight, compared with the weedy rice parents, although the hybrids produced less filled seeds per plant than their weedy parents. Seeds from the F(1) hybrids had higher germination rates and produced more seedlings than the weedy parents, which correlated positively with 1 000-seed weight. The crop-weed hybrids demonstrated a generally enhanced relative performance than their weedy rice parents in our field experiments. These findings indicate that transgenes from GM rice can persist to and introgress into weedy rice populations through recurrent crop-to-weed gene flow with the aid of slightly increased relative fitness in F(1) hybrids.

Diversity and population dynamics of pests and predators in irrigated rice fields with treated and untreated pesticide.

PubMed

Rattanapun, W

2012-01-01

The monitoring of rice pests and their predators in pesticide untreated and treated rice fields was conducted at the southern of Thailand. Twenty-two species in 15 families and 6 orders of rice pests were sampled from untreated rice field. For treated rice field, 22 species in 14 families and 5 orders of rice pest were collected. Regardless of treatment type, dominant species and individual number of rice pest varied to physiological stage of rice. Lepidopteran pests had highest infestation during the vegetative stage of rice growth, while hemipteran pests composed of hopper species (Hemipetra: Auchenorrhyncha) and heteropteran species (Hemiptera: Heteroptera) were dominant groups during the reproductive stage and grain formation and ripening stage of rice growth. In contrast, dominant species of predator did not change throughout rice growing season. There were 35 species in 25 families and seven orders and 40 species in 29 families and seven orders of predators collected from untreated and treated rice field, respectively. Major predators of both rice fields were Micraspis discolor (Fabricius) (Coleoptera: Coccinellidae), Tetragnatha sp. (Araneae: Tetragnathidae) and Agriocnemis pygmaea Rambur (Odonata: Agrionidae). The population dynamic of predators were not related with rice pest population in both treatments. However, the fluctuation of population pattern of rice pests in the untreated treatment were more distinctly synchronized with their predators than that of the treated treatment. There were no significant differences in the total number of rice pest and predator between two treatments at vegetative and reproductive stages of rice growth. Untreated rice field had a higher population number of predator and a lower population number of rice pest than that of treated rice field during grain formation and ripening stages. These results indicated the ago-ecosystem balance in rice fields could be produced through minimal pesticide application, in order to allow

Field trial of GABA-fortified rice plants and oral administration of milled rice in spontaneously hypertensive rats.

PubMed

Kowaka, Emi; Shimajiri, Yasuka; Kawakami, Kouhei; Tongu, Miki; Akama, Kazuhito

2015-06-01

Hypertension is one of the most critical risk factors accompanying cardiovascular diseases. γ-Aminobutyric acid (GABA) is a non-protein amino acid that functions as a major neurotransmitter in mammals and also as a blood-pressure lowering agent. We previously produced GABA-fortified rice lines of a popular Japonica rice cultivar 'Koshihikari' by genetic manipulation of GABA shunt-related genes. In the study reported here, we grew these same novel rice lines in a field trial and administered the milled rice orally to rats. The yield parameters of the transgenic rice plants were almost unchanged compared to those of untransformed cv. 'Koshihikari' plants, while the rice grains of the transgenic plants contained a high GABA content (3.5 g GABA/kg brown rice; 0.75-0.85 GABA g/kg milled rice) in a greenhouse trial. Oral administration of a diet containing 2.5% GABA-fortified rice, with a daily intake for 8 weeks, had an approximately 20 mmHg anti-hypertensive effect in spontaneous hypertensive rats but not in normotensive Wistar-Kyoto rats. These results suggest that GABA-fortified rice may be applicable as a staple food to control or prevent hypertension.

A comparison of spider communities in Bt and non-Bt rice fields.

PubMed

Lee, Sue Yeon; Kim, Seung Tae; Jung, Jong Kook; Lee, Joon-Ho

2014-06-01

To assess the potential adverse effects of a Bt rice line (Japonica rice cultivar, Nakdong) expressing a synthetic cry1Ac1 gene, C7-1-9-1-B, which was highly active against all larval stages of Cnaphalocrocis medinalis (Guenée) (Lepidoptera: Crambidae), we investigated the community structure of spiders in Bt and non-Bt rice fields during the rice-growing season in 2007 and 2008 in Chungcheongnam-do, Korea. Spiders were surveyed with a sweep net and suction device. Suction sampling captured more spiders, measured in terms of species level and abundance, than sweeping. Araneidae and Thomisidae were captured more by sweeping, and certain species were captured only by sweeping. These findings show that both suction and sweep sampling methods should be used because these methods are most likely complementary. In total, 29 species in 23 genera and nine families were identified from the 4,937 spiders collected, and both Bt and non-Bt rice fields showed a typical Korean spider assemblage. The temporal patterns of spider species richness and spider abundance were very similar between Bt and non-Bt rice, although significant differences in species richness were observed on a few occasions. Overall, spider community structure, including diversity, the dominant species, and abundance did not differ between Bt and non-Bt rice. The results of the study indicated that the transgenic Cry1Ac rice lines tested in this study had no adverse effects on the spider community structure of the rice fields.

[Ammonia volatilization loss of nitrogen fertilizer from rice field and wet deposition of atmospheric nitrogen in rice growing season].

PubMed

Su, Chengguo; Yin, Bin; Zhu, Zhaoliang; Shen, Qirong

2003-11-01

Plot and field experiments showed that the NH3 volatilization loss from rice field reached its maximum in 1-3 days after N-fertilization, which was affected by the local climate conditions (e.g., sun illumination, temperature, humidity, wind speed, and rainfall), fertilization time, and ammonium concentration in surface water of the rice field. The wet deposition of atmospheric nitrogen was correlated with the application rate of N fertilizer and the rainfall. The amount of nitrogen brought into soil or surface water by the wet deposition in rice growing season reached 7.5 kg.hm-2. The percent of NH4(+)-N in the wet deposition was about 39.8%-73.2%, with an average of 55.5%. There was a significant correlation of total ammonia volatilization loss with the average concentration of NH4(+)-N in wet deposition and total amount of wet deposition in rice growing season.

Automatic rice crop height measurement using a field server and digital image processing.

PubMed

Sritarapipat, Tanakorn; Rakwatin, Preesan; Kasetkasem, Teerasit

2014-01-07

Rice crop height is an important agronomic trait linked to plant type and yield potential. This research developed an automatic image processing technique to detect rice crop height based on images taken by a digital camera attached to a field server. The camera acquires rice paddy images daily at a consistent time of day. The images include the rice plants and a marker bar used to provide a height reference. The rice crop height can be indirectly measured from the images by measuring the height of the marker bar compared to the height of the initial marker bar. Four digital image processing steps are employed to automatically measure the rice crop height: band selection, filtering, thresholding, and height measurement. Band selection is used to remove redundant features. Filtering extracts significant features of the marker bar. The thresholding method is applied to separate objects and boundaries of the marker bar versus other areas. The marker bar is detected and compared with the initial marker bar to measure the rice crop height. Our experiment used a field server with a digital camera to continuously monitor a rice field located in Suphanburi Province, Thailand. The experimental results show that the proposed method measures rice crop height effectively, with no human intervention required.

A possible alternative method for collecting mosquito larvae in rice fields

PubMed Central

Robert, Vincent; Goff, Gilbert Le; Ariey, Frédéric; Duchemin, Jean-Bernard

2002-01-01

Background Rice fields are efficient breeding places for malaria vectors in Madagascar. In order to establish as easily as possible if a rice field is an effective larval site for anophelines, we compared classical dipping versus a net as methods of collecting larvae. Results Using similar collecting procedures, we found that the total number of anopheline larvae collected with the net was exactly double (174/87) that collected by dipping. The number of anopheline species collected was also greater with a net. Conclusions The net is an effective means of collecting anopheline larvae and can be used for qualitative ecological studies and to rapidly determine which rice fields are containing malaria vectors. PMID:12057018

Changing cultural landscape in post-productivism of rice field in Nyuh Kuning Village Bali

NASA Astrophysics Data System (ADS)

Maulidi, C.; Wulandari, L. D.

2017-06-01

Natural landscape in developing countries is facing a challenge due to economic growth, a cultural shift, and population dynamics. Farm land where is close to urban areas tending to be converted into more economically valuable spaces. Watershed Pakerisan listed as World Heritage of UNESCO, rich of cultural value on its landscape, especially the Subak, a traditional irrigation system, has a close relationship to the philosophy of Hindu-Bali culture. Nyuh Kuning, a village (local terms is Banjar) located adjacent to the Pakerisan Watershed, and has a spatial pattern in synergic ally connected with tradition, culture, and their religion. Rice field not only for economical but also its place to worship the Goddess (Dewi Sri). Rice Field in Nyuh Kuning declined significantly along past 10 years. The changing landscape of Nyuh Kuning traced through serial of aerial photographs from 2005 until 2015. Along with the broad decline of rice field, villager’s attachment on their cultural space is also changing. An economic motive pronounces a winner in the bargaining between the motives of economic value and cultural value in the Nyuh Kuning. Villagers revealed arguments that necessities nowadays prosecute high consumption, both for household and for education. Therefore conversion of rice fields to become more economical is understandable among communities. Villagers rent the rice fields to foreigners (migrants), and then foreigners take rice-fields as personal assets, not for the villagers (ritual activities and the cultural traditions) any longer. In theoritical term, villager’s emotional bond to the cultural landscape in post—productivism of rice field, is weakened. Wawedangan Desa and its complex cultural values are not part of their identity anymore. However, place dependence become the reason why the shifting place attachment is happening. Functional economic bond is mentioned as place dependence dominats in villager’s attachment. Certainly it’s not a

Determination of stable cesium and strontium in rice samples by inductively coupled plasma mass spectrometry

NASA Astrophysics Data System (ADS)

Srinuttrakul, W.; Yoshida, S.

2017-06-01

For long-term radiation dose assessment models, food ingestion is one of the major exposure pathways to human. In general, the stable isotopes can serve as analogues of radioisotopes. In this study, rice samples were collected from 30 paddy fields in Si Sa Ket, Yasothon and Roi Et in the northeast of Thailand in November 2014. The concentrations of stable cesium (Cs-133) and strontium (Sr-88) in polished rice were determined by inductively coupled plasma mass spectrometry (ICP-MS). The standard reference material of rice flour (NIST 1568a) with spiked Cs and Sr was used to validate the analytical method. The concentration of Cs in polished rice from Si Sa Ket, Yasothon and Roi Et was 0.158 ± 0.167 mg kg-1, 0.090 ± 0.117 mg kg-1 and 0.054 ± 0.031 mg kg-1, respectively. The concentration of Sr in polished rice from Si Sa Ket, Yasothon and Roi Et was 0.351 ± 0.108 mg kg-1, 0.364 ± 0.215 mg kg-1 and 0.287 ± 0.102 mg kg-1, respectively. Comparison of the results with Japanese data before the Fukushima Di-ichi nuclear power plant accident showed that the concentrations of both Cs and Sr for Thai rice were higher than those for Japanese rice.

Monoclonal antibody-based serological assays and immunocapture-RT-PCR for detecting Rice dwarf virus in field rice plants and leafhopper vectors.

PubMed

Wu, Jianxiang; Ni, Yuequn; Liu, Huan; Ding, Ming; Zhou, Xueping

2014-01-01

Rice dwarf virus (RDV) causes Rice dwarf disease, which leads to considerable losses in rice production in Asia. Purified RDV virions were used as the immunogen to prepare monoclonal antibodies (mAbs). Three murine mAbs against RDV were prepared. Plate-trapped antigen enzyme-linked immunosorbent assay (PTA-ELISA), dot enzyme-linked immunosorbent assay (dot-ELISA) and immunocapture-RT-PCR (IC-RT-PCR) were then developed for sensitive, specific, and rapid detection of RDV in rice and leafhopper samples obtained in the field using the mAbs. The PTA-ELISA, dot-ELISA and IC-RT-PCR detected the virus in infected tissue crude extracts diluted at 1:81,920, 1:10,240 and 1:655,360 (w/v, g mL(-1)), in individual viruliferous rice green leafhopper crude extracts diluted at 1:25,600, 1:6400 and 1:3,276,800 (individual leafhopper/μL), respectively. 878 rice field samples and 531 leafhopper field samples from ten provinces of China were screened for the presence of RDV using the two serological assays and the IC-RT-PCR and the results indicated that 37 of the 878 rice samples and 22 of the 531 leafhopper samples were infected by RDV. All positive samples were from Yunnan Province, indicating that RDV is prevalent in this province, but not in the other nine provinces. The dot-ELISA is suitable for routine detection of large-scale rice and leafhopper samples in field surveys. Copyright © 2013 Elsevier B.V. All rights reserved.

[Reducing cadmium content of rice grains by means of flooding and a few problems].

PubMed

Kawasaki, Akira; Arao, Tomohito; Ishikawa, Satoru

2012-01-01

The effects of water management in rice paddies on the levels of cadmium (Cd) and arsenic (As) in Japanese rice grains were tested. In order to reduce the Cd concentration in rice grains, flooding for 3 weeks before and after heading was most effective, but this treatment increased As concentration considerably. Aerobic treatment was effective in reducing As concentration in rice grains, but increased Cd concentration markedly. In the pot experiment, flooding treatment after heading was more effective than flooding treatment before heading in reducing both Cd and As concentrations in rice grains. The concentration of dimethylarsinic acid (DMA) in rice grains was very low under aerobic conditions, but increased in the continuous-flooding treatment. In the field experiment, the grain As concentration in the case of flooding for 3 weeks before and after heading was higher than that in the case of intermittent irrigation. The ratios of DMA to the total As concentration were 3-52% in the pot experiment and 7-13% in the field experiment.

Transfer function control strategy of Subak rice field land and agricultural development in Denpasar city

NASA Astrophysics Data System (ADS)

Lanya, Indayati; Netera Subadiyasa, N.; Sardiana, Ketut; Putu Ratna Adi, Gst.

2017-01-01

The success of tourism development in Bali gave a negative impact on Subak rice fields, especially on land convertion over 2579 ha year-1 (2002-2013) to the area awakened. Denpasar city has lost rice fields 185 ha year-1 and six Subak, as well as potentially losing 10 Subak, as a result of the allocation of space in the region in the Spatial Planing. UNESCO, in 2012 the establishment of Subak as a cultural heritage. Most Subak rice fields designated as an Urban Green Open Space ( UGOS). Satellite image Iconos 2002, World 2015 View Coverage of Denpasar, and ArcGIS 10.3 software used for mapping the balance of rice field and violation of land use in the area of UGOS. The control strategy over the convertion of spatial land-based environment is done through zoning map. Land conversion of rice fields for 13 years (2002-2015) in Denpasar (572.76 ha), comes standard acreage of rice fields in 2015. Denpasar city has experienced of food deficits, even in the UGOS has awakened 96.04 ha (24.04 ha year-1). A period of 50 years into the future, rice fields which needs to be protected 872.83 ha, buffer area 984.77 ha, and can be converted 499.81 ha.

Seasonal variation of carbon dioxide and methane exchange between rice paddy fields and atmosphere in Japan

NASA Astrophysics Data System (ADS)

Kokubo, R.

2017-12-01

Rice paddy fields spread throughout Asia and play an important role in terms of regulating greenhouse gases on the ground. Rice paddies have the potential to either increase or decrease the net balance of greenhouse gases in the atmosphere. In the rice growth period, rice paddy fields are sources of CH4, whereas they generally act as a sink of CO2. However, the behavior of greenhouse gases during fallow periods has not been well understood. A field experiment was conducted at a rice paddy field in Fuchu, central Japan in 2014. We evaluated CO2 and CH4 fluxes in the rice paddy field using the eddy covariance method. Except for 20 days after transplanting (DAT), temporal CO2 fluxes showed negative values during a rice growth period whereas they showed positive values throughout a fallow period. The positive CO2 fluxes at 2 emissions by respiration of rice plants and soil microorganisms than CO2 uptake by photosynthesis of rice plants. In the middle of the growing season at around DAT=50, CO2 emission became dominant again because flooded water was temporarily drained in the rice paddy field. Seasonal CH4 fluxes during a growth period were regulated by water management and plant growth stages. During a fallow period, however, the field was kept a non-flooded condition that resulted in an aerobic soil condition and thus very low CH4 emission.

Model development for nutrient loading estimates from paddy rice fields in Korea.

PubMed

Jeon, Ji-Hong; Yoon, Chun G; Ham, Jong-Hwa; Jung, Kwang-Wook

2004-01-01

A field experiment was performed to evaluate water and nutrient balances in paddy rice culture operations during 2001-2002. The water balance analysis indicated that about half (50-60%) of the total outflow was lost by surface drainage, with the remainder occurring by evapotranspiration (490-530 mm). The surface drainage from paddy fields was mainly caused by rainfall and forced-drainage, and in particular, the runoff during early rice culture periods depends more on the forced-drainage due to fertilization practices. Most of the total phosphorus (T-P) inflow was supplied by fertilization at transplanting, while the total nitrogen (T-N) inflow was supplied by the three fertilizations, precipitation. and from the upper paddy field, which comprised 13-33% of the total inflow. Although most of the nutrient outflow was attributed to plant uptake. nutrient loss by surface drainage was substantial, comprising 20% for T-N and 10% for T-P. Water and nutrient balances indicate that reduction of surface drainage from paddy rice fields is imperative for nonpoint source pollution control. The simplified computer model, PADDIMOD, was developed to simulate water and nutrient (T-N and T-P) behavior in the paddy rice field. The model predicts daily ponded water depth, surface drainage, and nutrient concentrations. It was formulated with a few equations and simplified assumptions, but its application and a model fitness test indicated that the simulation results reasonably matched the observed data. It is a simple and convenient planning model that could be used to evaluate BMPs of paddy rice fields alone or in combination with other complex watershed models. Application of the PADDIMOD to other paddy rice fields with different agricultural environments might require further calibration and validation.

Waste rice seed in conventional and stripper-head harvested fields in California: Implications for wintering waterfowl

USGS Publications Warehouse

Fleskes, Joseph P.; Halstead, Brian J.; Casazza, Michael L.; Coates, Peter S.; Kohl, Jeffrey D.; Skalos, Daniel A.

2012-01-01

Waste rice seed is an important food for wintering waterfowl and current estimates of its availability are needed to determine the carrying capacity of rice fields and guide habitat conservation. We used a line-intercept method to estimate mass-density of rice seed remaining after harvest during 2010 in the Sacramento Valley (SACV) of California and compared results with estimates from previous studies in the SACV and Mississippi Alluvial Valley (MAV). Posterior mean (95% credible interval) estimates of total waste rice seed mass-density for the SACV in 2010 were 388 (336–449) kg/ha in conventionally harvested fields and 245 (198–307) kg/ha in stripper-head harvested fields; the 2010 mass-density is nearly identical to the mid-1980s estimate for conventionally harvested fields but 36% lower than the mid-1990s estimate for stripped fields. About 18% of SACV fields were stripper-head harvested in 2010 vs. 9–15% in the mid-1990s and 0% in the mid-1980s; but due to a 50% increase in planted rice area, total mass of waste rice seed in SACV remaining after harvest in 2010 was 43% greater than in the mid-1980s. However, total mass of seed-eating waterfowl also increased 82%, and the ratio of waste rice seed to seed-eating waterfowl mass was 21% smaller in 2010 than in the mid-1980s. Mass-densities of waste rice remaining after harvest in SACV fields are within the range reported for MAV fields. However, because there is a lag between harvest and waterfowl use in the MAV but not in the SACV, seed loss is greater in the MAV and estimated waste seed mass-density available to wintering waterfowl in SACV fields is about 5–30 times recent MAV estimates. Waste rice seed remains an abundant food source for waterfowl wintering in the SACV, but increased use of stripper-head harvesters would reduce this food. To provide accurate data on carrying capacities of rice fields necessary for conservation planning, trends in planted rice area, harvest method, and postharvest field

Analysis of hyperspectral field radiometric data for monitoring nitrogen concentration in rice crops

NASA Astrophysics Data System (ADS)

Stroppiana, D.; Boschetti, M.; Confalonieri, R.; Bocchi, S.; Brivio, P. A.

2005-10-01

Monitoring crop conditions and assessing nutrition requirements is fundamental for implementing sustainable agriculture. Rational nitrogen fertilization is of particular importance in rice crops in order to guarantee high production levels while minimising the impact on the environment. In fact, the typical flooded condition of rice fields can be a significant source of greenhouse gasses. Information on plant nitrogen concentration can be used, coupled with information about the phenological stage, to plan strategies for a rational and spatially differentiated fertilization schedule. A field experiment was carried out in a rice field Northern Italy, in order to evaluate the potential of field radiometric measurements for the prediction of rice nitrogen concentration. The results indicate that rice reflectance is influenced by nitrogen supply at certain wavelengths although N concentration cannot be accurately predicted based on the reflectance measured at a given wavelength. Regression analysis highlighted that the visible region of the spectrum is most sensitive to plant nitrogen concentration when reflectance measures are combined into a spectral index. An automated procedure allowed the analysis of all the possible combinations into a Normalized Difference Index (NDI) of the narrow spectral bands derived by spectral resampling of field measurements. The derived index appeared to be least influenced by plant biomass and Leaf Area Index (LAI) providing a useful approach to detect rice nutritional status. The validation of the regressive model showed that the model is able to predict rice N concentration (R2=0.55 [p<0.01] RRMSE=29.4; modelling efficiency close to the optimum value).

Effect of rice-cooking water to the daily arsenic intake in Bangladesh: results of field surveys and rice-cooking experiments.

PubMed

Ohno, K; Matsuo, Y; Kimura, T; Yanase, T; Rahman, M H; Magara, Y; Matsushita, T; Matsui, Y

2009-01-01

The effect of rice-cooking water to the daily arsenic intake of Bangladeshi people was investigated. At the first field survey, uncooked rice and cooked rice of 29 families were collected. Their arsenic concentrations were 0.22+/-0.11 and 0.26+/-0.15 mg/kg dry wt, respectively. In 15 families, arsenic concentration in rice increased after cooking. Good correlation (R(2)=0.89) was observed between arsenic in rice-cooking water and the difference of arsenic concentration in rice by cooking. In the second survey, we collected one-day duplicated food of 18 families. As a result, we estimated that six of 18 families likely used the arsenic contaminated water for cooking rice even they drank less arsenic-contaminated water for drinking purpose. We also conducted rice-cooking experiments in the laboratory, changing arsenic concentration in rice-cooking water. Clear linear relationships were obtained between the arsenic in rice-cooking water and the difference of arsenic concentration in rice by cooking. Factors that affect arsenic concentration in cooked rice are suggested as follows: (1) arsenic concentration in uncooked rice, (2) that in rice-cooking water, (3) difference in water content of rice before and after cooking, and (4) types of rice, especially, the difference between parboiled and non-parboiled rice.

Phytochemical profile of a Japanese black-purple rice.

PubMed

Pereira-Caro, Gema; Watanabe, Shin; Crozier, Alan; Fujimura, Tatsuhito; Yokota, Takao; Ashihara, Hiroshi

2013-12-01

Black-purple rice is becoming popular with health conscious food consumers. In the present study, the secondary metabolites in dehulled black-purple rice cv. Asamurasaki were analysed using HPLC-PDA-MS(2). The seeds contained a high concentration of seven anthocyanins (1400 μg/g fresh weight) with cyanidin-3-O-glucoside and peonidin-3-O-glucoside predominating. Five flavonol glycosides, principally quercetin-3-O-glucoside and quercetin-3-O-rutinoside, and flavones were detected at a total concentration of 189 μg/g. The seeds also contained 3.9 μg/g of carotenoids consisting of lutein, zeaxanthin, lycopene and β-carotene. γ-Oryzanol (279 μg/g) was also present as a mixture of 24-methylenecycloartenol ferulate, campesterol ferulate, cycloartenol ferulate and β-sitosterol ferulate. No procyanidins were detected in this variety of black-purple rice. The results demonstrate that the black-purple rice in the dehulled form in which it is consumed by humans contains a rich heterogeneous mixture of phytochemicals which may provide a basis for the potential health benefits, and highlights the possible use of the rice as functional food. Copyright © 2013 Elsevier Ltd. All rights reserved.

Effects of Bt-transgenic rice cultivation on planktonic communities in paddy fields and adjacent ditches.

PubMed

Liu, Yongbo; Liu, Fang; Wang, Chao; Quan, Zhanjun; Li, Junsheng

2016-09-15

The non-target effects of transgenic plants are issues of concern; however, their impacts in cultivated agricultural fields and adjacent natural aquatic ecosystems are poorly understood. We conducted field experiments during two growing seasons to determine the effects of cultivating Bacillus thuringiensis (Bt)-transgenic rice on the phytoplankton and zooplankton communities in a paddy field and an adjacent ditch. Bt toxin was detected in soil but not in water. Water quality was not significantly different between non-Bt and Bt rice fields, but varied among up-, mid- and downstream locations in the ditch. Cultivation of Bt-transgenic rice had no effects on zooplankton communities. Phytoplankton abundance and biodiversity were not significantly different between transgenic and non-transgenic rice fields in 2013; however, phytoplankton were more abundant in the transgenic rice field than in the non-transgenic rice field in 2014. Water quality and rice type explained 65.9% and 12.8% of this difference in 2014, respectively. Phytoplankton and zooplankton were more abundant in mid- and downstream, than upstream, locations in the ditch, an effect that we attribute to water quality differences. Thus, the release of Bt toxins into field water during the cultivation of transgenic crops had no direct negative effects on plankton community composition, but indirect effects that alter environmental conditions should be taken into account during the processes of management planning and policymaking. Copyright © 2016 Elsevier B.V. All rights reserved.

Field Trials Reveal Ecotype-Specific Responses to Mycorrhizal Inoculation in Rice.

PubMed

Diedhiou, Abdala Gamby; Mbaye, Fatou Kine; Mbodj, Daouda; Faye, Mathieu Ndigue; Pignoly, Sarah; Ndoye, Ibrahima; Djaman, Koffi; Gaye, Souleymane; Kane, Aboubacry; Laplaze, Laurent; Manneh, Baboucarr; Champion, Antony

2016-01-01

The overuse of agricultural chemicals such as fertilizer and pesticides aimed at increasing crop yield results in environmental damage, particularly in the Sahelian zone where soils are fragile. Crop inoculation with beneficial soil microbes appears as a good alternative for reducing agricultural chemical needs, especially for small farmers. This, however, requires selecting optimal combinations of crop varieties and beneficial microbes tested in field conditions. In this study, we investigated the response of rice plants to inoculation with arbuscular mycorrhizal fungi (AMF) and plant growth promoting bacteria (PGPB) under screenhouse and field conditions in two consecutive seasons in Senegal. Evaluation of single and mixed inoculations with AMF and PGPB was conducted on rice (Oryza sativa) variety Sahel 202, on sterile soil under screenhouse conditions. We observed that inoculated plants, especially plants treated with AMF, grew taller, matured earlier and had higher grain yield than the non-inoculated plants. Mixed inoculation trials with two AMF strains were then conducted under irrigated field conditions with four O. sativa varieties, two O. glaberrima varieties and two interspecific NERICA varieties, belonging to 3 ecotypes (upland, irrigated, and rainfed lowland). We observed that the upland varieties had the best responses to inoculation, especially with regards to grain yield, harvest index and spikelet fertility. These results show the potential of using AMF to improve rice production with less chemical fertilizers and present new opportunities for the genetic improvement in rice to transfer the ability of forming beneficial rice-microbe associations into high yielding varieties in order to increase further rice yield potentials.

Field Trials Reveal Ecotype-Specific Responses to Mycorrhizal Inoculation in Rice

PubMed Central

Diedhiou, Abdala Gamby; Mbaye, Fatou Kine; Mbodj, Daouda; Faye, Mathieu Ndigue; Pignoly, Sarah; Ndoye, Ibrahima; Djaman, Koffi; Gaye, Souleymane; Kane, Aboubacry; Laplaze, Laurent; Manneh, Baboucarr; Champion, Antony

2016-01-01

The overuse of agricultural chemicals such as fertilizer and pesticides aimed at increasing crop yield results in environmental damage, particularly in the Sahelian zone where soils are fragile. Crop inoculation with beneficial soil microbes appears as a good alternative for reducing agricultural chemical needs, especially for small farmers. This, however, requires selecting optimal combinations of crop varieties and beneficial microbes tested in field conditions. In this study, we investigated the response of rice plants to inoculation with arbuscular mycorrhizal fungi (AMF) and plant growth promoting bacteria (PGPB) under screenhouse and field conditions in two consecutive seasons in Senegal. Evaluation of single and mixed inoculations with AMF and PGPB was conducted on rice (Oryza sativa) variety Sahel 202, on sterile soil under screenhouse conditions. We observed that inoculated plants, especially plants treated with AMF, grew taller, matured earlier and had higher grain yield than the non-inoculated plants. Mixed inoculation trials with two AMF strains were then conducted under irrigated field conditions with four O. sativa varieties, two O. glaberrima varieties and two interspecific NERICA varieties, belonging to 3 ecotypes (upland, irrigated, and rainfed lowland). We observed that the upland varieties had the best responses to inoculation, especially with regards to grain yield, harvest index and spikelet fertility. These results show the potential of using AMF to improve rice production with less chemical fertilizers and present new opportunities for the genetic improvement in rice to transfer the ability of forming beneficial rice-microbe associations into high yielding varieties in order to increase further rice yield potentials. PMID:27907023

Root microbiota shift in rice correlates with resident time in the field and developmental stage.

PubMed

Zhang, Jingying; Zhang, Na; Liu, Yong-Xin; Zhang, Xiaoning; Hu, Bin; Qin, Yuan; Xu, Haoran; Wang, Hui; Guo, Xiaoxuan; Qian, Jingmei; Wang, Wei; Zhang, Pengfan; Jin, Tao; Chu, Chengcai; Bai, Yang

2018-06-01

Land plants in natural soil form intimate relationships with the diverse root bacterial microbiota. A growing body of evidence shows that these microbes are important for plant growth and health. Root microbiota composition has been widely studied in several model plants and crops; however, little is known about how root microbiota vary throughout the plant's life cycle under field conditions. We performed longitudinal dense sampling in field trials to track the time-series shift of the root microbiota from two representative rice cultivars in two separate locations in China. We found that the rice root microbiota varied dramatically during the vegetative stages and stabilized from the beginning of the reproductive stage, after which the root microbiota underwent relatively minor changes until rice ripening. Notably, both rice genotype and geographical location influenced the patterns of root microbiota shift that occurred during plant growth. The relative abundance of Deltaproteobacteria in roots significantly increased overtime throughout the entire life cycle of rice, while that of Betaproteobacteria, Firmicutes, and Gammaproteobacteria decreased. By a machine learning approach, we identified biomarker taxa and established a model to correlate root microbiota with rice resident time in the field (e.g., Nitrospira accumulated from 5 weeks/tillering in field-grown rice). Our work provides insights into the process of rice root microbiota establishment.

Can rice field channels contribute to biodiversity conservation in Southern Brazilian wetlands?

PubMed

Maltchik, Leonardo; Rolon, Ana Silvia; Stenert, Cristina; Machado, Iberê Farina; Rocha, Odete

2011-12-01

Conservation of species in agroecosystems has attracted attention. Irrigation channels can improve habitats and offer conditions for freshwater species conservation. Two questions from biodiversity conservation point of view are: 1) Can the irrigated channels maintain a rich diversity of macrophytes, macroinvertebrates and amphibians over the cultivation cycle? 2) Do richness, abundance and composition of aquatic species change over the rice cultivation cycle? For this, a set of four rice field channels was randomly selected in Southern Brazilian wetlands. In each channel, six sample collection events were carried out over the rice cultivation cycle (June 2005 to June 2006). A total of 160 taxa were identified in irrigated channels, including 59 macrophyte species, 91 taxa of macroinvertebrate and 10 amphibian species. The richness and abundance of macrophytes, macroinvertebrates and amphibians did not change significantly over the rice cultivation cycle. However, the species composition of these groups in the irrigation channels varied between uncultivated and cultivated periods. Our results showed that the species diversity found in the irrigation channels, together with the permanence of water enables these man-made aquatic networks to function as important systems that can contribute to the conservation of biodiversity in regions where the wetlands were converted into rice fields. The conservation of the species in agriculture, such as rice field channels, may be an important alternative for biodiversity conservation in Southern Brazil, where more than 90% of wetland systems have already been lost and the remaining ones are still at high risk due to the expansion of rice production.

Dioxin pollution disrupts reproduction in male Japanese field mice.

PubMed

Ishiniwa, Hiroko; Sakai, Mizuki; Tohma, Shimon; Matsuki, Hidenori; Takahashi, Yukio; Kajiwara, Hideo; Sekijima, Tsuneo

2013-11-01

Dioxins cause various adverse effects in animals including teratogenesis, induction of drug metabolizing enzymes, tumor promotion, and endocrine disruption. Above all, endocrine disruption is known to disturb reproduction in adult animals and may, also seriously impact their offspring. However, most previous studies have quantified the species-specific accumulation of dioxins, whereas few studies have addressed the physiological impacts of dioxins on wildlife, such as reduced reproductive function. Here we clarify an effect of endocrine disruption caused by dioxins on the Japanese field mouse, Apodemus speciosus. Japanese field mice collected from various sites polluted with dioxins accumulated high concentrations of dioxins in their livers. Some dioxin congeners, especially, 1,2,3,4,6,7,8-heptachlorodibenzo-p-dioxin, 3,3',4,4',5-pentachloro biphenyl, 1,2,3,4,6,7,8-heptachlorodibenzofuran, and octachlorodibenzo-p-dioxin, which showed high biota-soil accumulation factors, contributed to concentration of dioxins in mouse livers with an increase of accumulation of total dioxins. As for physiological effects on the Japanese field mouse, high levels of cytochrome P450 1A1 (CYP1A1) mRNA, a drug metabolizing enzyme induced by dioxins, were found in the livers of mice captured at polluted sites. Furthermore, at such sites polluted with dioxins, increased CYP1A1 expression coincided with reduced numbers of active spermatozoa in mice. Thus, disruption in gametogenesis observed in these mice suggests that dioxins not only negatively impact reproduction among Japanese field mice, but might also act as a kind of selection pressure in a chemically polluted environment.

Identification of rice field using Multi-Temporal NDVI and PCA method on Landsat 8 (Case Study: Demak, Central Java)

NASA Astrophysics Data System (ADS)

Sukmono, Abdi; Ardiansyah

2017-01-01

Paddy is one of the most important agricultural crop in Indonesia. Indonesia’s consumption of rice per capita in 2013 amounted to 78,82 kg/capita/year. In 2017, the Indonesian government has the mission of realizing Indonesia became self-sufficient in food. Therefore, the Indonesian government should be able to seek the stability of the fulfillment of basic needs for food, such as rice field mapping. The accurate mapping for rice field can use a quick and easy method such as Remote Sensing. In this study, multi-temporal Landsat 8 are used for identification of rice field based on Rice Planting Time. It was combined with other method for extract information from the imagery. The methods which was used Normalized Difference Vegetation Index (NDVI), Principal Component Analysis (PCA) and band combination. Image classification is processed by using nine classes, those are water, settlements, mangrove, gardens, fields, rice fields 1st, rice fields 2nd, rice fields 3rd and rice fields 4th. The results showed the rice fields area obtained from the PCA method was 50,009 ha, combination bands was 51,016 ha and NDVI method was 45,893 ha. The accuracy level was obtained PCA method (84.848%), band combination (81.818%), and NDVI method (75.758%).

Understanding ecosystem services provided by rice fields

USDA-ARS?s Scientific Manuscript database

While the concept of ecosystem services is not new, there is revived interest in how these services should be measured, monitored, and valued. Focused research over the last four years has provided insight into the mitigation capabilities of rice fields in the lower Mississippi River Valley of the ...

Study of mosquito fauna in rice ecosystems around Hanoi, northern Vietnam.

PubMed

Ohba, Shin-ya; Van Soai, Nguyen; Van Anh, Dinh Thi; Nguyen, Yen T; Takagi, Masahiro

2015-02-01

Species of the Culex vishnui subgroup, Cx. fuscocephala and Cx. gelidus, which are known Japanese encephalitis (JE) vectors, are distributed in rice agroecosystems in Asian countries. Hence, although ecological studies of rice agroecosystems in northern Vietnam are necessary, very few integrated studies of breeding habitats of mosquitoes, including JE vectors, have been conducted. We carried out a field study and investigated the mosquito fauna in six rice production areas in northern Vietnam during the rainy and dry seasons of 2009. Mosquitoes and potential mosquito predators were collected from aquatic habitats by using larval dippers. We collected 1780 Culex individuals (including 254 Cx. tritaeniorhynchus; 113 Cx. vishnui, 58 Cx. vishnui complex, consisting of Cx. vishnui and Cx. pseudovishnui; 12 Cx. gelidus; 1 Cx. bitaeniorhynchus; and 1 Cx. fuscocephala), 148 Anopheles individuals (including 5 An. vagus), 1 Mansonia annulifera, and 1 Mimomyia chamberlaini during the rainy season. During the dry season, we collected 176 Culex individuals (including 33 Cx. vishnui, 24 Cx. tritaeniorhynchus, 8 Cx. vishnui complex, and 1 Cx. gelidus) and 186 Anopheles individuals (including 9 An. tessellatus, 2 An. kochi, and 2 An. barbumbrosus). We found mosquitoes in all aquatic habitats, namely, rice fields, ditches, ponds, wetlands, irrigation canals, and rice nurseries, and Cx. tritaeniorhynchus and Cx. vishnui complex were found in all the above six areas. Heteroptera such as Micronecta, Veliidae, and Pleidae were abundant and widely distributed in both the seasons. The abundance of mosquito larvae was higher in the rice fields, ditches, and ponds during the rainy season than during the dry season. Cx. tritaeniorhynchus, Cx. vishnui complex, Cx. fuscocephala, and Cx. gelidus were abundant in rice agroecosystems (rice fields, ditches, ponds, and wetlands) in northern Vietnam, and their abundance was high during the rainy season. These findings deepen our understanding of

Air pollutant emissions from rice straw open field burning in India, Thailand and the Philippines.

PubMed

Gadde, Butchaiah; Bonnet, Sébastien; Menke, Christoph; Garivait, Savitri

2009-05-01

Rice is a widely grown crop in Asia. China (30%) and India (21%) contribute to about half of the world's total rice production. In this study, three major rice-producing countries in Asia are considered, India, Thailand and the Philippines (the later two contributing 4% and 2% of the world's rice production). Rice straw is one of the main field based residues produced along with this commodity and its applications vary widely in the region. Although rice production practises vary from one country to another, open burning of straw is a common practice in these countries. In this study, an approach was followed aiming at (a) determining the quantity of rice straw being subject to open field burning in those countries, (b) congregating pollutant specific emissions factors for rice straw burning, and (c) quantifying the resulting air pollutant emissions. Uncertainties in the results obtained as compared to a global approach are also discussed.

Spatial Field Variability Mapping of Rice Crop using Clustering Technique from Space Borne Hyperspectral Data

NASA Astrophysics Data System (ADS)

Moharana, S.; Dutta, S.

2015-12-01

Precision farming refers to field-specific management of an agricultural crop at a spatial scale with an aim to get the highest achievable yield and to achieve this spatial information on field variability is essential. The difficulty in mapping of spatial variability occurring within an agriculture field can be revealed by employing spectral techniques in hyperspectral imagery rather than multispectral imagery. However an advanced algorithm needs to be developed to fully make use of the rich information content in hyperspectral data. In the present study, potential of hyperspectral data acquired from space platform was examined to map the field variation of paddy crop and its species discrimination. This high dimensional data comprising 242 spectral narrow bands with 30m ground resolution Hyperion L1R product acquired for Assam, India (30th Sept and 3rd Oct, 2014) were allowed for necessary pre-processing steps followed by geometric correction using Hyperion L1GST product. Finally an atmospherically corrected and spatially deduced image consisting of 112 band was obtained. By employing an advanced clustering algorithm, 12 different clusters of spectral waveforms of the crop were generated from six paddy fields for each images. The findings showed that, some clusters were well discriminated representing specific rice genotypes and some clusters were mixed treating as a single rice genotype. As vegetation index (VI) is the best indicator of vegetation mapping, three ratio based VI maps were also generated and unsupervised classification was performed for it. The so obtained 12 clusters of paddy crop were mapped spatially to the derived VI maps. From these findings, the existence of heterogeneity was clearly captured in one of the 6 rice plots (rice plot no. 1) while heterogeneity was observed in rest of the 5 rice plots. The degree of heterogeneous was found more in rice plot no.6 as compared to other plots. Subsequently, spatial variability of paddy field was

Heavy Metal Content in Terraced Rice Fields at Sruwen Tengaran Semarang - Indonesia

NASA Astrophysics Data System (ADS)

Hindarwati, Yulis; Soeprobowati, Tri Retnaningsih; Sudarno

2018-02-01

The presence of heavy metal on agricultural soils can be caused not only natural factors but also due to human intervention. Differences in management and lack of understanding of farmers in the production input of fertilizers and pesticides ensued in land ravaged. Periodic testing of paddy fields is necessary to minimize the contaminants from being absorbed by plants that will have an impact on health decline. The purpose of the assessment was to identify the heavy metal content in the terraced rice field in Sruwen Village, Tengaran District, Semarang Regency. Survey was conducted in February 2017. Sampling on terraced rice fields of different heights consisted of upper, middle, and upper down. Taken as many as eight single points and composed at a depth of 0-20 cm and 20-40 cm. The identification results showed that heavy metal content of Pb, Cd, and Cu were present at all altitudes. Heavy Metals Pb and Cd at a depth of 0-20 cm were higher from 20-40 cm in the upper and lower rice fields but lower in the middle rice field. Cu heavy metal at a depth of 0-20 cm was higher than 20-40 cm in all altitude land. The heavy metal content of Pb, Cd, and Cu was still below the heavy metal standard set by the European Union and India.

Testing climate-smart irrigation strategies to reduce methane emissions from rice fields

NASA Astrophysics Data System (ADS)

Runkle, B.; Suvocarev, K.; Reba, M. L.

2017-12-01

Approximately 11% of the global 308 Tg CH4 anthropogenic emissions are currently attributed to rice cultivation. In this study, the impact of water conservation practices on rice field CH4 emissions was evaluated in Arkansas, the leading state in US rice cultivation. While conserving water, the Alternate Wetting and Drying (AWD) irrigation practice can also reduce CH4 emissions through the deliberate, periodic introduction of aerobic conditions. Seasonal CH4emissions from a pair of adjacent, production-sized rice fields were estimated and compared during the 2015 to 2017 growing seasons using the eddy covariance method on each field. The fields were alternately treated with continuous flood (CF) and AWD irrigation. In 2015, the seasonal cumulative carbon losses by CH4 emission were 30.3 ± 6.3 and 141.9 ± 8.6 kg CH4-C ha-1 for the AWD and CF treatments, respectively. Data from 2016 and 2017 will be analyzed and shown within this presentation; an initial view demonstrates consistent findings to 2015. When accounting for differences in field conditions and soils, the AWD practice is attributable to a 36-51% reduction in seasonal emissions. The substantial decrease in CH4 emissions by AWD supports previous chamber-based research and offers strong evidence for the efficacy of AWD in reducing CH4 emissions in Arkansas rice production. The AWD practice has enabled the sale of credits for carbon offsets trading and this new market could encourage CH4 emissions reductions on a national scale. These eddy covariance towers are being placed into a regional perspective including crop and forest land in the three states comprising the Mississippi Delta: Arkansas, Mississippi, and Louisiana.

Formation of Guaiacol by Spoilage Bacteria from Vanillic Acid, a Product of Rice Koji Cultivation, in Japanese Sake Brewing.

PubMed

Ito, Toshihiko; Konno, Mahito; Shimura, Yoichiro; Watanabe, Seiei; Takahashi, Hitoshi; Hashizume, Katsumi

2016-06-08

The formation of guaiacol, a potent phenolic off-odor compound in the Japanese sake brewing process, was investigated. Eight rice koji samples were analyzed, and one contained guaiacol and 4-vinylguaiacol (4-VG) at extraordinarily high levels: 374 and 2433 μg/kg dry mass koji, respectively. All samples contained ferulic and vanillic acids at concentrations of mg/kg dry mass koji. Guaiacol forming microorganisms were isolated from four rice koji samples. They were identified as Bacillus subtilis, B. amyloliquefaciens/subtilis, and Staphylococcus gallinarum using 16S rRNA gene sequence. These spoilage bacteria convert vanillic acid to guaiacol and ferulic acid to 4-VG. However, they convert very little ferulic acid or 4-VG to guaiacol. Nine strains of koji fungi tested produced vanillic acid at the mg/kg dry mass koji level after cultivation. These results indicated that spoilage bacteria form guaiacol from vanillic acid, which is a product of koji cultivation in the sake brewing process.

Use of banker plant system for sustainable management of the most important insect pest in rice fields in China.

PubMed

Zheng, Xusong; Lu, Yanhui; Zhu, Pingyang; Zhang, Facheng; Tian, Junce; Xu, Hongxing; Chen, Guihua; Nansen, Christian; Lu, Zhongxian

2017-04-03

To meet the World's food demand, there is a growing need for sustainable pest management practices. This study describes the results from complementary laboratory and field studies of a "banker plant system" for sustainable management of the rice brown planthopper (BPH) (Nilaparvata lugens Stål) - the economically most important rice pest in Asian rice growing areas. The banker plant system consisted of planting a grass species, Leersia sayanuka, adjacent to rice fields. L. sayanuka is the host plant of a planthopper, Nilaparvata muiri. An egg parasitoid, Anagrus nilaparvatae, parasitizes eggs of both BPH and N. muiri, and its establishment and persistence are improved through plantings of L. sayanuka and thereby attraction of N. muiri. Laboratory results showed that BPH was unable to complete its life cycle on L. sayanuka, and N. muiri could not complete its life cycle on rice. Thus, planting L. sayanuka did not increase the risk of planthopper damage to rice fields. Field studies showed that BPH densities were significantly lower in rice fields with banker plant system compared to control rice fields without banker plant system.

Estimating shorebird populations during spring stopover in rice fields of the Louisiana and Texas Gulf Coastal Plain

USGS Publications Warehouse

Norling, Wayne; Jeske, Clinton W.; Thigpen, Tyler F.; Chadwick, Paul C.

2012-01-01

Migrating shorebird populations using approximately 2% of Louisiana and Texas Gulf Coastal rice fields were surveyed during spring migration (March–May of 1997 and 1998) using biweekly stratified random surveys conducted at 50 roadside survey points and approximately 30,000 shorebirds were observed. Shorebird counts were extrapolated and almost 1.4 million birds in 1997 and over 1.6 million birds of 31 species in 1998 were estimated to use rice field habitat for stopover sites in Louisiana and Texas. Greater than 50% of the estimated North American populations were estimated to use rice field habitats for five species, including a species of concern, Buff-breasted Sandpiper (Tryngites subruficollis) at 187%. Because of predictability of suitable rice field habitat acreage, timing of field preparation and water availability, coastal rice prairies are identified as critical spring migration stopover sites.

Hydrology of a groundwater-irrigated rice field in Bangladesh: Seasonal and daily mechanisms of infiltration

NASA Astrophysics Data System (ADS)

Neumann, Rebecca B.; Polizzotto, Matthew L.; Badruzzaman, A. Borhan M.; Ali, M. Ashraf; Zhang, Zhongyuan; Harvey, Charles F.

2009-09-01

Flow through a groundwater-irrigated rice field in Bangladesh was characterized with data collected from a transect of tensiometers and time domain reflectometry sensors, novel tracer tests, infiltration tests, soil core analyses, and calculated water budgets. The combined data captured the dynamic hydrologic behavior of the rice field over an entire growing season, which included many irrigation events. Recharge to the aquifer flowed from the surface of the rice field through preferential flow paths located in the subsoil beneath the plowed surface of the field and in the bunds, the raised boundaries around the perimeter of the field. Water that remained within the soil matrix did not recharge the aquifer. Bund flow was the dominant loss for the field because the bulk hydraulic conductivity of the soil beneath the bunds was greater than that in the plowed and planted region of the rice field. Each year, farmers plow the rice fields, destroying cracks and decreasing the conductivity of the shallow soil, but leave the bunds unplowed because they follow property boundaries. We determined bund flow with a daily water balance and confirmed its importance by comparing irrigation losses among fields of different sizes and geometries and hence different ratios of perimeter to area. The perimeter-to-area ratio predicted the fraction of water lost down the bunds for these and other fields located throughout Southeast Asia. Finally, we determined the economic and environmental benefits of reducing bund flow.

No effect of Bt-transgenic rice litter on the meiobenthos community in field ditches.

PubMed

Liu, Yongbo; Jiang, Wanxiang; Liang, Yuyong; Zhao, Caiyun; Li, Junsheng

2017-06-01

The non-target effect of Bacillus thuringiensis (Bt) toxins in aquatic ecosystems is crucial to improve the present assessment of Bt-transgenic plants, particularly where crops are cultivated near aquatic ecosystems. We conducted decomposition experiments during two growing seasons to determine the effects of Bt-transgenic rice litter with and without insecticide application on the meiobenthos communities in a field ditch. The community composition of meiobenthos colonised on leaf litter was not significantly different between Bt and non-Bt rice. The abundance of meiobenthos colonising leaves differed between insecticide application and control, and this insecticide effect interacted with rice type. No Bt toxin was detected in field ditch water. Leaf decomposition and nutrient content were comparable for both Bt and non-Bt rice with or without insecticide application. Bt-transgenic rice litter had no effect on the meiobenthos community composition in field ditches, but the chronic persistence of transgenic litter in nature needs to be taken into account at large scales in aquatic ecosystems. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry.

Seasonal assessment of greenhouse gas emission from irrigated lowland rice field under infrared warming

USDA-ARS?s Scientific Manuscript database

Rice fields are considered as one of the major sources of methane (CH4), and they also emit nitrous oxide (N2O). A field experiment was conducted at the International Rice Research Institute, Philippines, in 2010 – 2011 using a temperature free-air controlled enhancement (T-FACE) system. Our object...

Unravelling trophic subsidies of agroecosystems for biodiversity conservation: food consumption and nutrient recycling by waterbirds in Mediterranean rice fields.

PubMed

Navedo, Juan G; Hahn, Steffen; Parejo, Manuel; Abad-Gómez, José M; Gutiérrez, Jorge S; Villegas, Auxiliadora; Sánchez-Guzmán, Juan M; Masero, José A

2015-04-01

Waterbirds can reallocate a considerable amount of nutrients within agricultural fields and between agriculture sites and wetlands. However their effects on biogeochemical cycles have rarely been quantified. We estimated bird numbers, diet (from stable isotope analysis), food supply, and the food consumption on rice fields by overwintering waterbirds in one of the most important areas for rice production in southwestern Europe and a key area for various migrating and resident waterbird species. Herein, we modelled the nutrient (N and P) recycling in rice fields, and their transport to reservoirs. The energy consumption by waterbirds (96,605±18,311 individuals) on rice fields during winter averaged at 89.9±39.0 kJ·m(-2), with its majority (89.9%) belonging to foraging on rice seeds. Thus, the birds removed about 26% of rice seeds leftover after harvest (estimated in 932.5±504.7 seeds·m(-2) in early winter) wherein common cranes and dabbling ducks (four species) were the most important consumers. Waterbirds foraging and roosting in the rice fields recycled more than 24.1 (1.0 kg·ha(-1)) of N and an additional 5.0 tons (0.2 kg·ha(-1)) of P in the Extremadura's rice fields during winter. Additionally, we estimated that 2.3 tons of N and 550 kg of P were removed from rice fields and transported to reservoirs. The seasonal foraging of wildlife should result in a direct benefit for rice farmers by improving nutrient recycling through defecation by waterbirds with respect to artificial fertilisation. Additionally, rice fields located in the cranes' core wintering areas can provide sufficient food supply to induce habitat shift from their traditional wintering habitat in 'dehesas' to rice fields, which causes indirect socioeconomic benefit through reduced acorn consumption by cranes. Our modelling approach may thus be especially helpful for management decisions regarding rice agroecosystems in areas which are also important for the conservation of migratory

Field evidence for the potential of Rhodobacter capsulatus as Biofertilizer for flooded rice.

PubMed

Gamal-Eldin, Hosny; Elbanna, Khaled

2011-02-01

In a previous study, we evaluated the effects of inoculating rice plants with the phototrophic purple nonsulfur bacterium Rhodobacter capsulatus (Rc) on growth and yield of rice in pots and lysimeter experiments and the results obtained have been highly encouraging. In this study, we carried out two field experiments: one in the experimental farm of the Faculty of Agriculture, Fayoum University, and the second in a farmer's field in Kafr El-sheikh, to assess the effects of Rc on growth and yield of rice in comparison and in combination with chemical nitrogen fertilizer (CNF) and farmyard manure. The results indicated that both biological and grain yields in all the Rc inoculated treatments were significantly higher than those in the uninoculated corresponding treatments in both fields. With regard to grain yield, the major factor for determining the effectiveness of any agricultural treatment, inoculation with Rc in combination with 50% of the recommended CNF rate gave a grain yield that was statistically equivalent to that obtained with 100% of the recommended CNF rate. These results provide a clear evidence for the potential of Rc as biofertilizer for flooded rice under field conditions.

PCDD/F EMISSIONS FROM BURNING WHEAT AND RICE FIELD RESIDUE

EPA Science Inventory

The paper presents the first known values for emissions of polychlorinated dibenzodioxins and dibenzofurans (PCDDs/Fs) from combustion of agricultural field biomass. Wheat and rice straw stubble collected from two western U.S. states were tested in a field burn simulation to dete...

Use of banker plant system for sustainable management of the most important insect pest in rice fields in China

PubMed Central

Zheng, Xusong; Lu, Yanhui; Zhu, Pingyang; Zhang, Facheng; Tian, Junce; Xu, Hongxing; Chen, Guihua; Nansen, Christian; Lu, Zhongxian

2017-01-01

To meet the World’s food demand, there is a growing need for sustainable pest management practices. This study describes the results from complementary laboratory and field studies of a “banker plant system” for sustainable management of the rice brown planthopper (BPH) (Nilaparvata lugens Stål) – the economically most important rice pest in Asian rice growing areas. The banker plant system consisted of planting a grass species, Leersia sayanuka, adjacent to rice fields. L. sayanuka is the host plant of a planthopper, Nilaparvata muiri. An egg parasitoid, Anagrus nilaparvatae, parasitizes eggs of both BPH and N. muiri, and its establishment and persistence are improved through plantings of L. sayanuka and thereby attraction of N. muiri. Laboratory results showed that BPH was unable to complete its life cycle on L. sayanuka, and N. muiri could not complete its life cycle on rice. Thus, planting L. sayanuka did not increase the risk of planthopper damage to rice fields. Field studies showed that BPH densities were significantly lower in rice fields with banker plant system compared to control rice fields without banker plant system. PMID:28367978

Microwave Backscatter and Attenuation Dependence of Leaf Area Index for Flooded Rice Fields

NASA Technical Reports Server (NTRS)

Durden, Stephen L.; Morrissey, Leslie A.; Livingston, Gerald P.

1995-01-01

Wetlands are important for their role in global climate as a source of methane and other reduced trace gases. As part of an effort to determine whether radar is suitable for wetland vegetation monitoring, we have studied the dependence of microwave backscatter and attenuation on leaf area index (LAI) for flooded rice fields. We find that the radar return from a flooded rice field does show dependence on LAI. In particular, the C-band VV cross section per unit area decreases with increasing LAI. A simple model for scattering from rice fields is derived and fit to the observed HH and VV data. The model fit provides insight into the relation of backscatter to LAI and is also used to calculate the canopy path attenuation as a function of LAI.

Modulation of DNA methylation machineries in Japanese rice fish (Oryzias latipes) embryogenesis by ethanol and 5-azacytidine.

PubMed

Dasmahapatra, Asok K; Khan, Ikhlas A

2016-01-01

As a sequel of our investigations on the impact of epigenome in inducing fetal alcohol spectrum disorder (FASD) phenotypes in Japanese rice fish, we have investigated on several DNA methylation machinery genes including DNA methyl transferase 3ba (dnmt3ba) and methyl binding proteins (MBPs), namely, mbd1b, mbd3a, mbd3b, and mecp2 at the transcription level. Studies were made during normal development, from 0day post fertilization (dpf) to hatching, and also exposing the fertilized eggs to ethanol or a DNMT inhibitor, 5-azacytidine (5-azaC). We observed that during development, all these genes followed distinct expression patterns, generally high mRNA copies in early phases (0-1dpf) and significantly low mRNA copies prior to or after hatching. Ethanol (100-500mM, 0-2dpf) was unable to alter any of these mRNAs in 2dpf; additional four day (2-6dpf) maintenance of these embryos in ethanol-free environment, on 6dpf, was also unable to establish any significant difference in these mRNA levels in comparison with the corresponding controls. However, continuous exposure of fertilized eggs in 300mM ethanol, 0-6dpf, showed significantly high mRNA copies only in MBPs (mbd1b, mbd3a, mbd3b, mecp2). 5-azaC (2mM) on 2dpf was able to enhance only mbd3b mRNA. Removal of 5-azaC and maintenance of these embryos in clean medium, 2-6dpf, showed significantly enhanced mbd3b and mecp2 mRNAs compared to corresponding controls on 6dpf. Our studies showed that in Japanese rice fish embryogenesis both ethanol and 5-azaC have the potential to specifically modulate the developmental rhythm of DNA methylation machineries. Published by Elsevier Inc.

First records of a field experiment on fertilizer effects on methane emission from rice fields in Hunan-Province (PR China)

NASA Astrophysics Data System (ADS)

Wassmann, R.; Wang, M. X.; Shangguan, X. J.; Xie, X. L.; Shen, R. X.; Wang, Y. S.; Papen, H.; Rennenberg, H.; Seiler, W.

Fertilizer effects on methane emission from Chinese rice fields were investigated by a praxis-oriented approach applying balanced amendments of N, P and K. The data set obtained covered the emission rates of app. one month in early rice and one month in late rice 1991. An intercomparison between the 4 treatments showed pronounced differences in the magnitudes of methane emission rates. The combined organic/mineral fertilizer application, commonly used as local farming practice, resulted in relatively high seasonal averages of methane emission rates (26.5 mg CH4 m-2 h-1 in early rice and 50.1 mg CH4 m-2 h-1 in late rice). The lowest emission rates were observed in the plot with pure mineral fertilization (6.5 mg CH4 m-2 h-1 in early rice and 14.3 mg CH4 m-2 h-1 in late rice). Pure organic fertilizers by unfermented substances yielded the highest methane emission rates of all field trials (38.6 mg CH4 m-2 h-1 in early rice and 56.2 CH4 m-2 h-1 in late rice). The fertilization with fermented material derived from biogas generators resulted in substantially lower emission rates than the other trials with organic amendments, the seasonal averages corresponded to 15.9 mg CH4 m-2 h-1 (early rice) and 22.5 mg CH4 m-2 h-1 (late rice). Interpretation of the results can be obtained from the different potentials of these fertilizers for methane production. Based on this concept the different methane emission rates observed with organic/mineral, pure mineral and pure unfermented-organic fertilizers could directly be attributed to the different quantities of organic matter incorporated into the soil. The low methane emission from the plot treated with fermented material could be explained by a depletion of potential methane precursors resulting from the preceding fermentation. The results of this investigation provide evidence that the extensive use of specific chemical fertilizers and the application of sludge from the operation of biogas generators could lead to a net

Methylmercury dynamics in Upper Sacramento Valley rice fields with low background soil mercury levels

USGS Publications Warehouse

Tanner, K. Christy; Windham-Myers, Lisamarie; Marvin-DiPasquale, Mark C.; Fleck, Jacob; Tate, Kenneth W.; Linquist, Bruce A.

2018-01-01

Few studies have considered how methylmercury (MeHg, a toxic form of Hg produced in anaerobic soils) production in rice (Oryza sativa L.) fields can affect water quality, and little is known about MeHg dynamics in rice fields. Surface water MeHg and total Hg (THg) imports, exports, and storage were studied in two commercial rice fields in the Sacramento Valley, California, where soil THg was low (25 and 57 ng g−1). The median concentration of MeHg in drainage water exiting the fields was 0.17 ng g−1 (range: <0.007–2.1 ng g−1). Compared with irrigation water, drainage water had similar MeHg concentrations, and lower THg concentrations during the growing season. Significantly elevated drainage water MeHg and THg concentrations were observed in the fallow season compared with the growing season. An analysis of surface water loads indicates that fields were net importers of both MeHg (76–110 ng m−2) and THg (1947–7224 ng m−2) during the growing season, and net exporters of MeHg (35–200 ng m−2) and THg (248–6496 ng m−2) during the fallow season. At harvest, 190 to 700 ng MeHg m−2 and 1400 to 1700 ng THg m−2 were removed from fields in rice grain. Rice straw, which contained 120 to 180 ng MeHg m−2 and 7000–10,500 ng m−2 THg was incorporated into the soil. These results indicate that efforts to reduce MeHg and THg exports in rice drainage water should focus on the fallow season. Substantial amounts of MeHg and THg were stored in plants, and these pools should be considered in future studies.

Rice evapotranspiration at the field and canopy scales under water-saving irrigation

NASA Astrophysics Data System (ADS)

Liu, Xiaoyin; Xu, Junzeng; Yang, Shihong; Zhang, Jiangang

2018-04-01

Evapotranspiration (ET) is an important process of land surface water and thermal cycling, with large temporal and spatial variability. To reveal the effect of water-saving irrigation (WSI) on rice ET at different spatial scales and understand the cross spatial scale difference, rice ET under WSI condition at canopy (ETCML) and field scale (ETEC) were measured simultaneously by mini-lysimeter and eddy covariance (EC) in the rice season of 2014. To overcome the shortage of energy balance deficit by EC system, and evaluate the influence of energy balance closure degree on ETEC, ETEC was corrected as {ET}_{EC}^{*} by energy balance closure correction according to the evaporative fraction. Seasonal average daily ETEC, {ET}_{EC}^{*} and ETCML of rice under WSI practice were estimated as 3.12, 4.03 and 4.35 mm day-1, smaller than the values reported in flooded paddy fields. Daily ETEC, {ET}_{EC}^{*} and ETCML varied in a similar trends and reached the maximum in late tillering, then decreased along with the crop growth in late season. The value of ETEC was much lower than ETCML, and was frequently 1-2 h lagged behind ETCML. It indicated that the energy balance deficit resulted in underestimation of crop ET by EC system. The corrected value of {ET}_{EC}^{*} matched ETCML much better than ETEC, with a smaller RMSE (0.086 mm h-1) and higher R 2 (0.843) and IOA (0.961). The time lapse between {ET}_{EC}^{*} and ETCML was mostly shortened to less than 0.5 h. The multiple stepwise regression analysis indicated that net radiation ( R n) is the dominant factor for rice ET, and soil moisture ( θ) is another significant factor ( p < 0.01) in WSI rice fields. The difference between ETCML and {ET}_{EC}^{*} ({ET}_{CML} - {ET}_{EC}^{*}) were significantly ( p < 0.05) correlated with R n, air temperature ( T a), and air vapor pressure deficit ( D), and its partial correlation coefficients to R n and T a were slightly greater than D. Thus, R n, T a and D are important variables for

Greenhouse gas budget from a rice paddy field in the Albufera of Valencia, Spain.

NASA Astrophysics Data System (ADS)

Meijide, Ana; López-Ballesteros, Ana; Calvo-Roselló, Esperanza; López-Jiménez, Ramón; Recio-Huetos, Jaime; Calatayud, Vicent; Carrara, Arnaud; Serrano-Ortiz, Penelope

2017-04-01

Rice paddy fields are large sources of anthropogenic methane (CH4) and therefore many studies have assessed CH4 fluxes from rice paddy fields, mainly in Asia where most of the rice cultivation takes place. However, rice is also cultivated in the Mediterranean, where climatic and management conditions greatly differ. In the Albufera of Valencia, the largest freshwater lagoon in Spain, rice paddy fields have the particularity of being flooded not only while the rice grows, but also after the harvest during the winter. These flooding conditions might result in emissions which are very specific of this ecosystem, and cannot be extrapolated from other studies. We studied CH4 fluxes in a rice paddy field in the Albufera of Valencia at different stages of rice cultivation using the eddy covariance technique and static chambers. We additionally measured carbon dioxide (CO2), water fluxes and nitrous oxide (N2O) fluxes with eddy covariance and chamber methods respectively, in order to obtain a full greenhouse gas (GHG) budget. Our study also aimed at providing a mechanistic understanding of GHG emissions at different stages of rice cultivation, and therefore we also used the Enhanced and Normalized Vegetation Indexes (EVI and NDVI, respectively), derived from remote sensing images. The general ecosystem functioning encompasses three different phases. The first one, over the autumn and the winter, a biological dormancy period causes low CO2 emissions (ca. 1-5 µmol m-2 s-1), which coincides with the EVI and NDVI. The intermittent flooding taking place during this period is expected to cause CH4 emissions. Then, during the spring months (March-May), larger CO2 respiratory emissions take place during the daytime (> 5 µmol m-2 s-1) due to an increase in air temperature, which turn to neutral at the end of spring due to the start of photosynthesis by the rice. The third phase corresponds to the vegetation growth, when the net CO2 uptake increases gradually up to maximum CO2

Assessing farmers' community readiness towards the enhancement of natural enemy population in rice fields in Malacca

NASA Astrophysics Data System (ADS)

Fairuz, K.; Idris, A. G.; Syahrizan, S.; Hatijah, K.

2018-04-01

Malacca has committed to be a green technology state by the year 2020. Agriculture is one of the main industries that have been highlighted to achieve this goal especially rice farming activities. Some limitations for this issue have restricted the accomplishment of the plan including pesticide usage among rice farmers. The use of chemicals in rice field need to be reduced significantly in order to support the goal. One of the indicators to the successfulness of pesticide reduction is the increasing numbers of natural enemies' species abundance and population in the rice field. Natural enemies were important to regulate pest populations in rice field naturally. Farmers' readiness to participate in this issue is very important to ensure the successfulness. The level of readiness of farmers' community will determine whether they are ready or not to execute the plan. Unfortunately, such information in rice farmers' community was not properly measured. Thus this study was aimed to assess the readiness level of rice farmers' community to change in order to enhance natural enemies in their rice field. This study was adapting the CR model as its theoretical framework. Three rice farming area in Malacca were involved in this study namely, Jasin, Melaka Tengah and Alor Gajah. Questionnaires were used as major instrument and were randomly distributed to 224 farmers. Data collected were tested for their reliability, significance and level of readiness. Knowledge of issue, knowledge of effort and resources dimensions were found influencing the readiness dimension significantly, whilst the attitude and leadership dimensions were not. Generally, the level of readiness for farmers' community in Malacca was found in the sixth or initial stage, where some of them initially have started to practice a few related activities to enhance the natural enemies' population in their rice field. Continuous support and assistant from the leaders and local authorities are crucially needed in

Effects on wildlife of ethyl and methyl parathion applied to California rice fields

USGS Publications Warehouse

Custer, T.W.; Hill, E.F.; Ohlendorf, H.M.

1985-01-01

Selected rice fields on the Sacramento National Wildlife Refuge Complex were aerially sprayed one time during May or June 1982 with either ethyl (0.11 kg Al/ha) or methyl (0.84 kg AI/ha) parathion for control of tadpole shrimp, Triops longicaudatus. No sick or dead vertebrate wildlife were found or adjacent to the treated rice fields after spraying. Specimens of the following birds and mammals were assayed for brain cholinesterase (ChE) activity to determine exposure to either form of parathion; house mouse, Mus musculus; black-tailed jackrabbit, Lepus californicus; mallard, Anas platyrhynchos; ring-necked pheasant, Phasianus colchicus; American coot, Fulica americana; and red-winged blackbird, Agelaius phoeniceus. Both mice and pheasants from methyl parathion-treated fields had overall mean ChE activities that were significantly (P < 0.05) inhibited compared with controls, and 7, 40, 54 and 57% of individual blackbirds, pheasant, mice, and coots, respectively, had inhibited brain ChE activities (i.e., less than -2 SD of control mean). Although no overall species effect was detected for ethyl parathoid treatment, pheasants (43%), coots (33%), and mice (37%) had significantly inhibited brain ChE activities. Neither of the parathion treatment appeared acutely hazardous to wildlife in or adjacent to rice fields, but sufficient information on potential hazards was obtained to warrant caution in use of these chemicals, especially methyl parathion, in rice fields.

Evaluation of Rice Resistance to Southern Rice Black-Streaked Dwarf Virus and Rice Ragged Stunt Virus through Combined Field Tests, Quantitative Real-Time PCR, and Proteome Analysis.

PubMed

Wang, Zhenchao; Yu, Lu; Jin, Linhong; Wang, Wenli; Zhao, Qi; Ran, Longlu; Li, Xiangyang; Chen, Zhuo; Guo, Rong; Wei, Yongtian; Yang, Zhongcheng; Liu, Enlong; Hu, Deyu; Song, Baoan

2017-02-22

Diseases caused by southern rice black-streaked dwarf virus (SRBSDV) and rice ragged stunt virus (RRSV) considerably decrease grain yield. Therefore, determining rice cultivars with high resistance to SRBSDV and RRSV is necessary. In this study, rice cultivars with high resistance to SRBSDV and RRSV were evaluated through field trials in Shidian and Mangshi county, Yunnan province, China. SYBR Green I-based quantitative real-time polymerase chain reaction (qRT-PCR) analysis was used to quantitatively detect virus gene expression levels in different rice varieties. The following parameters were applied to evaluate rice resistance: acre yield (A.Y.), incidence of infected plants (I.I.P.), virus load (V.L.), disease index (D.I.), and insect quantity (I.Q.) per 100 clusters. Zhongzheyou1 (Z1) and Liangyou2186 (L2186) were considered the most suitable varieties with integrated higher A.Y., lower I.I.P., V.L., D.I. and I.Q. In order to investigate the mechanism of rice resistance, comparative label-free shotgun liquid chromatography tandem-mass spectrometry (LC-MS/MS) proteomic approaches were applied to comprehensively describe the proteomics of rice varieties' SRBSDV tolerance. Systemic acquired resistance (SAR)-related proteins in Z1 and L2186 may result in the superior resistance of these varieties compared with Fengyouxiangzhan (FYXZ).

Diazinon reduction and partitioning between water, sediment and vegetation in stormwater runoff mitigation through rice fields.

PubMed

Moore, Matthew T; Kröger, Robert; Cooper, Charles M; Cullum, Robert F; Smith, Sammie; Locke, Martin A

2009-11-01

Contamination of surface waters by pesticides is a concern in the United States and around the world. Innovative mitigation strategies are needed to remediate this potential environmental contaminant. One potential solution is to divert pesticide-laden drainage or surface water through agricultural rice fields. With a hydroperiod, hydrosoil and hydrophyte (rice), these systems serve essentially as a type of constructed wetland. In both summer and fall experiments, diazinon-amended water was diverted through two rice ponds at the University of Mississippi Field Station. Likewise, a non-vegetated control pond was amended with diazinon-laden water. Water, sediment and plant samples were taken spatially and temporally to determine the distribution of diazinon within systems. Outflow diazinon concentrations decreased significantly (P < 0.05) from inflow in both vegetated ponds for both preharvest and post-harvest experiments. Although sorption to rice plants was minimal in the overall mass distribution of diazinon (1-3%), temporal data indicated that diazinon concentrations reached the outflow sediment of the non-vegetated control twice as fast as in either vegetated (rice) system. In both vegetated systems, sediment diazinon concentrations decreased (77 and 100%) from inflow to outflow, while a decrease of <2% was noted in the non-vegetated control. Diversion of pesticide-contaminated water through rice fields demonstrated potential as a low-cost, environmentally efficient mitigation practice. Studies on these systems are continuing to evaluate the optimal chemical retention time for rice field mitigation, as well as diazinon transfer to rice grain seeds that may be used as a food source.

Impact of volunteer rice infestation on yield and grain quality of rice.

PubMed

Singh, Vijay; Burgos, Nilda R; Singh, Shilpa; Gealy, David R; Gbur, Edward E; Caicedo, Ana L

2017-03-01

Volunteer rice (Oryza sativa L.) grains may differ in physicochemical traits from cultivated rice, which may reduce the quality of harvested rice grain. To evaluate the effect of volunteer rice on cultivated rice, fields were surveyed in Arkansas in 2012. Cropping history that included hybrid cultivars in the previous two years (2010 and 2011) had higher volunteer rice infestation (20%) compared with fields planted previously with inbred rice (5.5%). The total grain yield of rice was reduced by 0.4% for every 1% increase in volunteer rice density. The grain quality did not change in fields planted with the same cultivar for three years. Volunteer rice density of at least 7.6% negatively impacted the head rice and when infestation reached 17.7%, it also reduced the rice grain yield. The protein and amylose contents of rice were not affected until volunteer rice infestation exceeded 30%. Crop rotation systems that include hybrid rice are expected to have higher volunteer rice infestation than systems without hybrid rice. It is predicted that, at 8% infestation, volunteer rice will start to impact head rice yield and will reduce total yield at 18% infestation. It could alter the chemical quality of rice grain at >30% infestation. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry.

Phenotype diversity analysis of red-grained rice landraces from Yuanyang Hani's terraced fields, China

NASA Astrophysics Data System (ADS)

Li, Lianjie; Cheng, Long

2017-10-01

There are many areas in the world have terraced fields, Yuanyang Rani's terraced fields are examples in the world, and their unique ecological diversity is beyond other terraced fields, rice landraces are very rich. In order to provide useful information for protection and utilization of red-grained rice landraces from Rani's terraced fields, 61 red-grained rice landraces were assessed based 20 quantitative traits. Principal component analysis (PCA) suggested that 20 quantitative characters could be simplified to seven principal components, and their accumulative contribution ration amounted to 78.699%. The first principal component (PC1) explained 18.375% of the total variance, which was contributed by filled grain number, 1000-grain weight, spikelets per panicle, secondary branch number, grain length, and grain thickness. PC2 accounted for 16.548% of the variance and featured flag leaf width, flag leaf area, panicle neck length and primary branch number. These traits were the most effective parameters to discriminate individuals. At the request of the proceedings editor and with the approval of all authors, article 040111 titled, "Phenotype diversity analysis of red-grained rice landraces from Yuanyang Hani's terraced fields, China," is being retracted from the public record due to the fact that it is a duplication of article 040110 published in the same volume.

[Characteristics of paddy field nitrogen leakage and runoff in rice-duck farming system].

PubMed

Yu, Xiang; Wang, Qiang-sheng; Wang, Shao-hua; Liu, Zheng-hui; Wang, Xia-wen; Ding, Yan-feng

2009-01-01

A field experiment was conducted to study the characteristics of paddy field nitrogen (N) leakage and runoff under rice-duck farming (MRD), conventional farming (MR), and conventional farming with flooding (CK). Comparing with that under MR, the paddy field under MRD had a notable decrease of N (especially NO3- -N) concentration in its leaked liquid; but this concentration was tended to be increased, compared with that under CK. After 7-9 days of fertilization, the NH4+ -N and NO3- -N concentrations in paddy field surface water were higher under MRD than under MR. However, owing to the no draining and the higher band, the paddy field under MRD had a notable reduction of drainage, resulting in a marked decrease of N runoff than that under MR. Comparing with MR, the paddy field under MRD had an addition of nitrogen supply from duck dung, a reduction of N leakage and runoff, a lesser application of chemical nitrogen fertilizer, and more nitrogen uptake by rice plant. Both the reduction of N input and that of N output in rice-duck farming system were nearly equal in quantity.

Rice emissions during field flooding and air pollution feedbacks across South Korea

NASA Astrophysics Data System (ADS)

So, C.; Diskin, G. S.; DiGangi, J. P.; Choi, Y.; Rana, M.; Hughes, S.; Blake, D. R.; Nault, B.; Schroeder, J.; Campuzano Jost, P.; Jimenez, J. L.; Kim, M. J.; Teng, A.; Crounse, J. D.; Wenneberg, P.; Kaser, L.; Mikoviny, T.; Müller, M.; Wisthaler, A.; Pusede, S. E.

2017-12-01

Nitrous oxide (N2O) and methane (CH4) are important long-lived greenhouse gases. Known anthropogenic sources of these gases include rice cultivation, which represents anywhere between 5% and 20% of methane emissions globally. Other volatile molecules are also produced by soil biogeochemistry when rice fields are flooded, including small organic oxygenates. Here, we use recent aircraft measurements from the KORUS-AQ experiment to describe controls over rice emissions of N2O and CH4 at regional-scales across the South Korean Peninsula. We also investigate potential emissions of molecular hydrogen and volatile alcohols and organic acids and consider the effect of aerosol nitrate and sulfate deposition on rice soil biogeochemistry on paddies downwind of polluted urban areas.

A field study of exposure to whole-body vibration due to agricultural machines in a full-time rice farmer over one year.

PubMed

Tsujimura, Hiroji; Taoda, Kazushi; Kitahara, Teruyo

2015-01-01

The aims of this study were to clarify in detail the levels of whole-body vibration (WBV) exposure from a variety of agricultural machines in a rice farmer over one year, and to evaluate the daily level of exposure compared with European and Japanese threshold limits. The subject was a full-time, male rice farmer. We measured vibration accelerations on the seat pan and at the seat base of four tractors with various implements attached, one rice-planting machine, two combine harvesters, produced by the same manufacturer, and one truck used for transportation of agricultural machines. The position and velocity of the machines were recorded in parallel with WBV measurements. In addition, during the year starting in April 2010, the subject completed a questionnaire regarding his work (date, place, content, hours worked, machines used). We calculated the daily exposure to WBV, A(8), on all the days on which the subject used the agricultural machines. The WBV magnitude in farm fields was relatively high during tasks with high velocity and heavy mechanical load on the machine, and had no dominant axis. The subject worked for 159 days using the agricultural machines during the year, and the proportion of days on which A(8) values exceeded the thresholds was 90% for the Japan occupational exposure limit and 24% for the EU exposure action value. Our findings emphasize the need for rice farmers to have health management strategies suited to the farming seasons and measures to reduce WBV exposure during each farm task.

Changes in the status of harvested rice fields in the Sacramento Valley, California: Implications for wintering waterfowl.

USGS Publications Warehouse

Miller, Michael R.; Garr, Jay D.; Coates, Peter S.

2010-01-01

Harvested rice fields provide critical foraging habitat for wintering waterfowl in North America, but their value depends upon post-harvest treatments. We visited harvested ricefields in the Sacramento Valley, California, during the winters of 2007 and 2008 (recent period) and recorded their observed status as harvested (standing or mechanically modified stubble), burned, plowed, or flooded. We compared these data with those from identical studies conducted during the 1980s (early period). We documented substantial changes in field status between periods. First, the area of flooded rice increased 4-5-fold, from about 15% to >40% of fields, because of a 3-4-fold increase in the percentage of fields flooded coupled with a 37-41% increase in the area of rice produced. Concurrently, the area of plowed fields increased from 35% of fields, burned fields declined from about 40% to 1%, and fields categorized as harvested declined from 22-54% to <15%. The increased flooding has likely increased access to food resources for wintering waterfowl, but this benefit may not be available to some goose species, and may be at least partially countered by the increase of plowed fields, especially those left dry, and the decrease of fields left as harvested.We encourage waterfowl managers to implement a rice field status survey in the Sacramento Valley and other North American rice growing regions as appropriate to support long-term monitoring programs and wetland habitat conservation planning for wintering waterfowl.

Feral rice from introgression of weedy rice genes into transgenic herbicide-resistant hybrid-rice progeny.

PubMed

Zhang, Jingxu; Kang, Ye; Valverde, Bernal E; Dai, Weimin; Song, Xiaoling; Qiang, Sheng

2018-06-05

Pollen-mediated herbicide-resistance transgene flow occurs bidirectionally between transgenic cultivated rice and weedy rice. The potential risk of weedy traits introgressing into hybrid rice is underestimated and poorly understood. Two of each glufosinate-resistant transgenic rice varieties and hybrid rice (F1) and their succeeding generations (F2-F4) were planted for three years in weedy-rice-free field plots adjacent to experimental weedy-rice fields. Weedy-rice-like (feral) plants, both glufosinate-resistant and with red-pericarp seed, were initially found only among the F3 generations of the two glufosinate-resistant transgenic hybrid rice. The composite fitness (an index based on eight productivity and weediness traits) of the feral progeny was significantly higher than that of glufosinate-resistant transgenic hybrid rice (the original female parent of feral progeny) under common monoculture garden conditions. Hybrid rice progeny segregated into individuals of variable height and extended flowering. Hybrid rice F2 generations had higher outcrossing rates by pollen reception (0.96%-1.65%) than their progenitors (0.07%-0.98%). Herbicide-resistant weedy rice can rapidly arise by pollen-mediated gene flow from weedy to transgenic hybrid rice. Their segregating pollen-receptive progeny pose greater agro-ecological risk than transgenic varieties. The safety assessment and management regulations for transgenic hybrid rice should take into account the risk of bidirectional gene flow.

Water management reduces greenhouse gas emissions in a Mediterranean rice paddy field

NASA Astrophysics Data System (ADS)

Gruening, Carsten; Meijide, Ana; Manca, Giovanni; Goded, Ignacio; Seufert, Guenther; Cescatti, Alessandro

2016-04-01

Rice paddy fields are one of the biggest anthropogenic sources of methane (CH4), the second most important greenhouse gas (GHG) after carbon dioxide (CO2). Therefore most studies on greenhouse gases (GHG) in these agricultural systems focus on the evaluation of CH4 production. However, there are other GHGs such as CO2 and nitrous oxide (N2O) also exchanged within the atmosphere. Since each of the GHGs has its own radiative forcing effect, the total GHG budget of rice cultivation and its global warming potential (GWP) must be assessed. For this purpose a field experiment was carried out in a Mediterranean rice paddy field in the Po Valley (Italy), the largest rice producing region in Europe. Ecosystem CO2 and CH4 fluxes were assessed using the eddy covariance technique, while soil respiration and soil CH4 and N2O fluxes were measured with closed chambers for two complete years. Combining all GHGs measured, the rice paddy field acted as a sink of -368 and -828 g CO2 eq m-2 year-1 in the first and second years respectively. Both years, it was a CO2 sink and a CH4 source, while the N2O contribution to the GWP was relatively small. Differences in the GHG budget between the two years of measurements were mainly caused by the greater CH4 emissions in the first year (37.4 g CH4 m-2 compared to 21.03 g CH4 m-2 in the second year), probably as a consequence of the drainage of the water table in the middle of the growing season during the second year, which resulted in lower CH4 emissions without significant increases of N2O and CO2 fluxes. However, midseason drainage also resulted in small decreases of yield, indicating that GHG budget studies from agricultural systems should consider carbon exports through the harvest. The balance between net GWP and carbon yield indicated a loss of carbon equivalents from the system, which was more than 30-fold higher in the first year. Our results therefore suggest that an adequate management of the water table has the potential to be an

Acetylcholinesterase inhibition and gill lesions in Rasbora caverii, an indigenous fish inhabiting rice field associated waterbodies in Sri Lanka.

PubMed

Wijeyaratne, W M D N; Pathiratne, Asoka

2006-10-01

The present study was aimed at applying condition factor (CF), brain acetylcholinesterase (AChE) and gill histology as biomarkers for detecting possible exposure/effect induced by pesticides in fish residing rice field associated waterbodies in Sri Lanka. Biomarkers of an indigenous fish, Rasbora caverii collected from five sampling sites including canals near rice fields, a river and a reservoir (the reference site) were evaluated at four sampling stages covering pesticide application periods during rice cultivation season in 2004. Results indicated that CF of the fish did not show significant alterations regardless of the sampling sites or sampling stages. Site specific differences in AChE activities of the fish were not evident either prior to application of pesticides or at 7 days after Paraquat application to the rice fields. Two days after the application of a mixture of Fenthion and Phenthoate to the rice fields, AChE activity of the fish collected from canals near rice fields was significantly depressed (65-75%) compared to the fish in the reference site. The activities remain depressed to 50-56% even at 65 days after the insecticides application. Laboratory studies showed that prior exposure of R. caverii to Paraquat (2 microg l(-1), 7 days) enhanced the extent of inhibition of brain AChE activity induced by Fenthion (3 microg l(-1)) or a mixture of Fenthion (3 microg l(-1)) and Phenthoate (5 microg l(-1)). Gills of fish collected from canals near rice fields exhibited abnormal multiple divisions at the tips of some secondary lamellae in addition to hyperplasia, hypertrophy and club shaped deformities. Results indicate that application of pesticides in rice culture could manifest a threat to native fish populations residing rice field associated waterbodies. The response of brain AChE and histological changes in the gills of R. caverii allowed differentiating sampling sites after insecticide applications to the rice fields. Hence, R. caverii may be

Metaproteomic identification of diazotrophic methanotrophs and their localization in root tissues of field-grown rice plants.

PubMed

Bao, Zhihua; Okubo, Takashi; Kubota, Kengo; Kasahara, Yasuhiro; Tsurumaru, Hirohito; Anda, Mizue; Ikeda, Seishi; Minamisawa, Kiwamu

2014-08-01

In a previous study by our group, CH4 oxidation and N2 fixation were simultaneously activated in the roots of wild-type rice plants in a paddy field with no N input; both processes are likely controlled by a rice gene for microbial symbiosis. The present study examined which microorganisms in rice roots were responsible for CH4 oxidation and N2 fixation under the field conditions. Metaproteomic analysis of root-associated bacteria from field-grown rice (Oryza sativa Nipponbare) revealed that nitrogenase complex-containing nitrogenase reductase (NifH) and the alpha subunit (NifD) and beta subunit (NifK) of dinitrogenase were mainly derived from type II methanotrophic bacteria of the family Methylocystaceae, including Methylosinus spp. Minor nitrogenase proteins such as Methylocella, Bradyrhizobium, Rhodopseudomonas, and Anaeromyxobacter were also detected. Methane monooxygenase proteins (PmoCBA and MmoXYZCBG) were detected in the same bacterial group of the Methylocystaceae. Because these results indicated that Methylocystaceae members mediate both CH4 oxidation and N2 fixation, we examined their localization in rice tissues by using catalyzed reporter deposition-fluorescence in situ hybridization (CARD-FISH). The methanotrophs were localized around the epidermal cells and vascular cylinder in the root tissues of the field-grown rice plants. Our metaproteomics and CARD-FISH results suggest that CH4 oxidation and N2 fixation are performed mainly by type II methanotrophs of the Methylocystaceae, including Methylosinus spp., inhabiting the vascular bundles and epidermal cells of rice roots. Copyright © 2014, American Society for Microbiology. All Rights Reserved.

Effects of wildlife of ethyl and methyl parathion applied to California USA rice fields

USGS Publications Warehouse

Custer, T.W.; Hill, E.F.; Ohlendorf, H.M.

1985-01-01

Selected rice fields on the Sacramento National Wildlife Refuge Complex were aerially sprayed one time during May or June 1982 with either ethyl (0.11 kg Al/ha) or methyl (0.84 kg AI/ha) parathion for control of tadpole shrimp, Triops longicaudatus. No sick or dead vertebrate wildlife were found or adjacent to the treated rice fields after spraying. Specimens of the following birds and mammals were assayed for brain cholinesterase (ChE) activity to determine exposure to either form of parathion; house mouse, Mus musculus; black-tailed jackrabbit, Lepus californicus; mallard, Anas platyrhynchos; ring-necked pheasant, Phasianus colchicus; American coot, Fulica americana; and red-winged blackbird, Agelaius phoeniceus. Both mice and pheasants from methyl parathion-treated fields had overall mean ChE activities that were significantly (P < 0.05) inhibited compared with controls, and 7, 40, 54 and 57% of individual blackbirds, pheasant, mice, and coots, respectively, had inhibited brain ChE activities (i.e., less than -2 SD of control mean). Although no overall species effect was detected for ethyl parathoid treatment, pheasants (43%), coots (33%), and mice (37%) had significantly inhibited brain ChE activities. Neither of the parathion treatment appeared acutely hazardous to wildlife in or adjacent to rice fields, but sufficient information on potential hazards was obtained to warrant caution in use of these chemicals, especially methyl parathion, in rice fields.

Field Evaluation of Four Spatial Repellent Devices Against Arkansas Rice-Land Mosquitoes

DTIC Science & Technology

2014-03-01

FIELD EVALUATION OF FOUR SPATIAL REPELLENT DEVICES AGAINST ARKANSAS RICE-LAND MOSQUITOES DAVID A. DAME,1 MAX V. MEISCH,2 CAROLYN N. LEWIS,2 DANIEL L... mosquitoes to locate a host. There are many commercially available spatial repellent products currently on the market. These products include...a large rice growing area where late-spring and summer agricultural irriga- tion generates dense mosquito populations. Spatial repellent devices

Toxicity evaluation of natural samples from the vicinity of rice fields using two trophic levels.

PubMed

Marques, Catarina R; Pereira, Ruth; Gonçalves, Fernando

2011-09-01

An ecotoxicological screening of environmental samples collected in the vicinity of rice fields followed a combination of physical and chemical measurements and chronic bioassays with two freshwater trophic levels (microalgae: Pseudokirchneriella subcapitata and Chlorella vulgaris; daphnids: Daphnia longispina and Daphnia magna). As so, water and sediment/soil elutriate samples were obtained from three sites: (1) in a canal reach crossing a protected wetland upstream, (2) in a canal reach surrounded by rice fields and (3) in a rice paddy. The sampling was performed before and during the rice culture. During the rice cropping, the whole system quality decreased comparatively to the situation before that period (e.g. nutrient overload, the presence of pesticides in elutriates from sites L2 and L3). This was reinforced by a significant inhibition of both microalgae growth, especially under elutriates. Contrary, the life-history traits of daphnids were significantly stimulated with increasing concentrations of water and elutriates, for both sampling periods.

The archaeobotany of Asian rice expansion and the development of wet-field cultivation

NASA Astrophysics Data System (ADS)

Fuller, D.

2008-12-01

Archaeobotanical evidence provides direct data on past human diet and agriculture, including a geographical and chronological framework for studying the expansion of rice agriculture. The growth of systematic archaeobotanical sampling in recent years has allowed for the past presence of rice to be seen in relation to cultivation of other crops and associated weeds. The weed flora provides a basis for inferring the nature of cultivation systems, whether rain-fed dry rice or wetland "paddy" rice, a key distinction for considerations of past methane production. Nevertheless, current data is very unevenly distributed. This poster will summarize available evidence for the origins and spread of rice in South Asia (India and Pakistan), and mainland and Island Southeast Asia deriving from an earlier Chinese domestication. Where possible, such as in India or China, the potential of the weed flora remains for distinguishing wetland rice crops will be summarized. In broad terms, although the origins of rice use and cultivation begins by or during the Middle Holocene (6000- 3000 BC), rice cultivation spread outside the regions of the wild progenitor after this time. Two phases of rice expansion can be distinguished. Phase 1, between 3000 and 1500 BC, introduced rice to Southeast Asia, probably under wetland cultivation, and the spread of dry rice over northern India and Pakistan. Phase 2, taking place between 1000 and 0 BC, sees the spread of rice throughout the Southern Indian Peninsula, with weed evidence suggesting irrigated wetland rice. Similarly, this period sees the spread of intensive paddy agriculture through Korea and Japan, but in Southeast Asia is probably related to a spread of rice in upland, dry field systems.

Estimation of the rice-planting field in Bangladesh by satellite remote sensing

NASA Astrophysics Data System (ADS)

Furuta, E.; Suzuki, G.; Yamassaki, M.; Teraoka, T.; Fujiwara, H.; Ogino, Y.; Akashi, M.; Lahrita, L.; Naruse, N.; Takahashi, Y.

2016-12-01

In Bangladesh, price of rice has been unstable due to a large increase in production. To control the price can become a political issue, because rice agriculture is one of the most important industries in Bangladesh, whereas the total area of the paddy field is accurately unknown, owing to unsustainable and on-site surveys for the area (1). Satellite remote sensing is an effective solution to research the all area of domestic paddy field. Microwave satellite imaging has a large merit to be observable regardless of the weather conditions, however, research institutions have been limited to observing continuously since the cost is high for developing countries, such as Bangladesh. This study aims to establish the way to grasp the paddy field using optical satellite images for free of charge (Landsat-8). We have focused on seasonal changes in the water and the vegetation indices obtained from paddy fields. We have performed image calculations of Normalized Difference Vegetation Index (NDVI) and Normalized Difference Water Index (NDWI) of the well-known paddy field in Bangladesh Rice Research Institute. We found that there are seasonal changes of NDVI and NDWI calculated from paddy field. The characteristics are as follows; the NDVI and the NDWI values varies by 0.17-0.25 up and 0.11-0.19 down, respectively, at the transition from the dry to the rainy season, on the other hand, the NDVI and the NDWI changes by 0.21-0.29 down and 0.09-0.17 up from the rainy to the dry season. These features make us to distinguish the paddy field from the other cultivated area. The decrease of NDVI means that rice bares, The increase of NDWI can be interpreted that the paddy field is covered with water for the preparation for planting it. Our estimated area of paddy field in Bangladesh (85,900km ) corresponds well with the previous reported value of 117,700km (1). We have established the way to grasp the paddy field using optical satellite images for free of charge, on the bases of the

Comparison of on-site field measured inorganic arsenic in rice with laboratory measurements using a field deployable method: Method validation.

PubMed

Mlangeni, Angstone Thembachako; Vecchi, Valeria; Norton, Gareth J; Raab, Andrea; Krupp, Eva M; Feldmann, Joerg

2018-10-15

A commercial arsenic field kit designed to measure inorganic arsenic (iAs) in water was modified into a field deployable method (FDM) to measure iAs in rice. While the method has been validated to give precise and accurate results in the laboratory, its on-site field performance has not been evaluated. This study was designed to test the method on-site in Malawi in order to evaluate its accuracy and precision in determination of iAs on-site by comparing with a validated reference method and giving original data on inorganic arsenic in Malawian rice and rice-based products. The method was validated by using the established laboratory-based HPLC-ICPMS. Statistical tests indicated there were no significant differences between on-site and laboratory iAs measurements determined using the FDM (p = 0.263, ά = 0.05) and between on-site measurements and measurements determined using HPLC-ICP-MS (p = 0.299, ά = 0.05). This method allows quick (within 1 h) and efficient screening of rice containing iAs concentrations on-site. Copyright © 2018 Elsevier Ltd. All rights reserved.

Seasonal variation of methane flux from coastal saline rice field with the application of different organic manures

NASA Astrophysics Data System (ADS)

Datta, A.; Yeluripati, Jagadeesh B.; Nayak, D. R.; Mahata, K. R.; Santra, S. C.; Adhya, T. K.

2013-02-01

A field experiment was conducted in an irrigated saline rice field of Gadakujang (a fishing hamlet of coastal Odisha, India, ravaged by the super cyclone of 1999 and cyclone BOB02 of 2006), to study the effects of locally available organic and fresh green manure amendment to the saline soil on methane (CH4) emission during wet and dry seasons using the conventional closed chamber flux measurement method. In a first report of this kind, CH4 emission vis-à-vis yield improvement of rice with different locally available organic manure application from coastal saline rice field soil of Odisha, is reported. The study confirms that CH4 flux from the saline soil planted to rice is significantly lower than that of irrigated inland non-saline rice field during both wet and dry seasons. Cumulative seasonal CH4 flux from different treatments of the coastal saline rice field ranged between 119.51 and 263.60 kg ha-1 during the wet season and 15.35-100.88 kg ha-1 during the dry season. Lower CH4 emission during the dry season may be attributed to the increased soil salinity (EC1:2) that went up from 0.76 dS m-1 during the wet season to 3.96 dS m-1 during the dry season. Annual CH4 emission per Mg grain yield was significantly low from plots treated with locally available green manure Morning glory (Ipomoea lacunosa) (17.27) with significantly high rice grain yield. Study indicates that Morning glory may be used as a potential green manure to increase grain yield and reduced CH4 emission from the coastal saline rice ecosystems of the tropics.

Transgenic rice plants expressing a fused protein of Cry1Ab/Vip3H has resistance to rice stem borers under laboratory and field conditions.

PubMed

Chen, Yang; Tian, Jun-Ce; Shen, Zhi-Chen; Peng, Yu-Fa; Hu, Cui; Guo, Yu-Yuan; Ye, Gong-Yin

2010-08-01

Six transgenic rice, Oryza sativa L., lines (G6H1, G6H2, G6H3, G6H4, G6H5, and G6H6) expressing a fused Cry1Ab/Vip3H protein, were evaluated for resistance against the Asiatic rice borer, Chilo suppressalis (Walker) (Lepidoptera: Crambidae), and the stem borer Sesamia inferens (Walker) (Lepidoptera: Noctuidae) in the laboratory and field. The bioassay results indicated that the mortality of Asiatic rice borer and S. inferens neonate larvae on six transgenic lines from seedling to filling stage was up to 100% at 168 h after infestation. The cumulative feeding area by Asiatic rice borer neonate larvae on all transgenic lines was significantly reduced compared with the untransformed parental 'Xiushui 110' rice. A 2-yr field evaluation showed that damage during the vegetative stage (deadheart) or during the reproductive stage (whitehead) caused by Asiatic rice borer and S. inferens for transgenic lines was much lower than the control. For three lines (G6H1, G6H2, and G6H6), no damage was found during the entire growing period. Estimation of fused Cry1Ab/Vip3H protein concentrations using PathoScreen kit for Bt-Cry1Ab/1Ac protein indicated that the expression levels of Cry1Ab protein both in main stems (within the average range of 0.006-0.073% of total soluble protein) and their flag leaves (within the average range of 0.001-0.038% of total soluble protein) were significantly different among six transgenic lines at different developmental stages. Both laboratory and field researches suggested that the transgenic rice lines have considerable potential for protecting rice from attack by both stem borers.

Focal species candidates for pesticide risk assessment in European rice fields: A review.

PubMed

Vallon, Martin; Dietzen, Christian; Laucht, Silke; Ludwigs, Jan-Dieter

2018-04-25

An assessment of potential risks of pesticides on wildlife is required during the process of product registration within Europe because of the importance of agricultural landscapes as wildlife habitats. Despite their peculiarity and their specific role as artificial wetlands, rice paddies are to date pooled with cereals in guidance documents on how to conduct risk assessments for birds and mammals in Europe. Hence, the focal species currently considered in risk assessments for rice paddies are those known from cereal fields and can therefore be expected to differ significantly from the species actually occurring in the wet environments of rice paddies. We present results of a comprehensive review on bird and mammal species regularly occurring in rice paddies during a time of potential pesticide exposure to identify appropriate focal species candidates for ecotoxicological pesticide risk assessment according to the European Food Safety Authority (EFSA). In addition, we present data on rice cultivation areas and agricultural practices in Europe to give background information supporting the species selection process. Our literature search identified a general scarcity of relevant data, particularly for mammals, which highlights the need for crop-specific focal species studies. However, our results clearly indicate that the relevant bird and mammal species in rice fields indeed differ strongly from the focal species used for the cereal risk assessment. They can thus be used as a baseline for more realistic wildlife risk assessments specific to rice and the development of a revised guidance document to bridge the gap for regulatory decision makers. Integr Environ Assess Manag 2018;00:000-000. © 2018 SETAC. © 2018 SETAC.

Remote sensing of rice fields and sea pollution by SIR-B

NASA Technical Reports Server (NTRS)

Fugono, N.; Furuhama, Y.; Takasugi, T.; Okamoto, K.; Fujita, M.; Yoshikado, S.; Masuko, H.; Shinozuka, T.; Inomata, H.; Shiro, I.

1984-01-01

Sensor calibration, rice fields, and sea pollution are to be investigated with respect to shuttle imaging radar-B (SIR-B). It is planned that the resolution characteristics of the SIR-B be evaluated, the sidelobe characteristics of the SIR-B be investigated, and the relationship between backscatter cross section and image intensity be established. The microwave-scattering characteristics of rice fields are to be studied using SIR-B data. The possibility of classifying crops from SIR-B data is to be explored. The characteristics of the radar image of oil-like surface films under several sea surface conditions are to be determined. The absolute measurement capability of the sea surface scattering cross section is to be estimated using the SIR.

Delay-tolerant mobile network protocol for rice field monitoring using wireless sensor networks

NASA Astrophysics Data System (ADS)

Guitton, Alexandre; Andres, Frédéric; Cardoso, Jarbas Lopes; Kawtrakul, Asanee; Barbin, Silvio E.

2015-10-01

The monitoring of rice fields can improve productivity by helping farmers throughout the rice cultivation cycle, on various issues: when to harvest, when to treat the crops against disease, when to increase the water level, how to share observations and decisions made in a collaborative way, etc. In this paper, we propose an architecture to monitor a rice field by a wireless sensor network. Our architecture is based on static sensor nodes forming a disconnected network, and mobile nodes communicating with the sensor nodes in a delay-tolerant manner. The data collected by the static sensor nodes are transmitted to mobile nodes, which in turn transmit them to a gateway, connected to a database, for further analysis. We focus on the related architecture, as well as on the energy-efficient protocols intended to perform the data collection.

Sun-induced chlorophyll fluorescence reveals strong representation of photosynthesis at ecosystem level in rice paddy field in Japan

NASA Astrophysics Data System (ADS)

Kato, T.; Tsujimoto, K.; Nasahara, K. N.; Akitsu, T.; Ono, K.; Miyata, A.

2015-12-01

Chlorophyll fluorescence emission from ecosystem induced by sunlight (Sun-Induced Fluorescence: SIF) is now a key factor to accurately estimate the ecosystem-level photosynthesis activity as suggested by satellite studies, and has been recently detected by satellites [Frankenberg et al., 2011; Guanter et al., 2012; Joiner et al., 2013] and measured at field stations [Daumard et al., 2010; Porcar-Castell, 2011]. However, the few example of field-based assessment on the representation ability reduces its value for the availability to better understand the dynamics in CO2uptake by land ecosystem. To elucidate the potential of SIF to estimate ecosystem GPP in typical Asian crop type, the canopy-top SIF was calculated from the spectrum data in Japanese rice paddy field in Mase in central Japan (36°03'N, 140°01'E, 11 m a.s.l.), and compared with eddy-tower measured GPP on half-hourly and daily bases during seven years from 2006 to 2012. The rice (Oriza sativa L.; cultivar Koshihikari) was transplanted in May and harvested in September normally. The SIF was estimated from the spectrums of downward Sun irradiance and upward canopy-reflected radiance measured at the height of 3m above ground by HemiSpherical Spectro-Radiometer (HSSR), consisting of the spectroradiometer (MS-700, Eko inc., Tokyo, Japan) with the full-width at half maximum (FWHM) of 10 nm and wavelength interval of 3.3 nm. The SIF around 760nm (O2-A band: Fs760) was calculated according to the Fraunhofer Line Depth principle [Maier et al., 2003] with several additional arrangements. The GPP increased almost linearly as both Fs760 and APAR (Absorbed Photosyntethically Active Radiation) increased based on monthly-averaged diurnal courses during the growing season in 2006. The slopes of their regression lines differed much among the months in APAR, but in Fs760. These nearly constant relationships among the months between GPP and Fs760 were kept for all the observation years. Daily averaged GPP and Fs760

Populations of Rice Grain Bug, Paraeuscosmetus pallicomis, (Hemiptera: Lygaeidae) in Weed-free Paddy Field, Weedy Paddy Field and Paddy Dykes.

PubMed

Abdullah, Tamrin; Nasruddin, Andi; Agus, Nurariaty

2017-07-01

Research on the populations of rice grain bug Paraeuscosmetus pallicomis Dallas (Hemiptera: Lygaeidae) in paddy field ecosystems was performed with the aim to determine the populations of rice grain bug in weed-free paddy field, weedy paddy field, and paddy dykes. Experiment was carried out in the village of Paccellekang in the district of Patallasang of Gowa Regency in South Sulawesi, Indonesia. Observations were performed during the milky grain stage (85 days after planting), the mature grain stage (105 days after planting), and one day after harvest (115 days after transplanting). Results showed that 85 days after the transplanting, the populations of rice grain bug was significantly higher in the weedy paddy field compared to weed-free field and paddy dykes with total numbers of 1.75, 3.53, and 0.31 insects per 2 hills, respectively. Similarly, 105 days after the transplanting, 2.53, 5.53, and 0.11 insects per hill, respectively. However, one day after the harvest (115 days after transplanting) the number of insects in weed-free field decreased, while in the dykes increased, and the weedy plot still had the highest number of insects per 2 hills. Our results suggested that weeds played an important role in regulating the bug population by providing alternative shelter and foods for the insect.

Reducing CH4 emission from rice paddy fields by altering water management

NASA Astrophysics Data System (ADS)

Sudo, S.; Itoh, M.

2010-12-01

Percentage of atmospheric methane emitted form rice paddy is estimated at 60Tg/yr (20 - 100Tg/yr) which is near 10% of total global methane emission of 535Tg/yr (410 - 660Tg) (IPCC(1995), and which is near 30% of anthropogenic CH4 emission. Thus, mitigation of CH4 emission is urgently required. CH4 in paddy soil is emanated by the activities of anaerobic bacteria which is called methane producer through reduction of CO2 or decomposition of acetic acid, and it is transported to atmosphere through soil or paddy water surface. It is effective to control methane emission from rice paddy that period is extended on intermittent drainage, composted rice straw is incorporated as fertilizer instead of flesh one, or other. However, empirical approach of these kinds of experiments had not been sufficient because such a kind of experiment required significant times and efforts. In this study, we conducted demonstrative experiments to verify the effects of water management method differences in order to reduce CH4 emission from rice paddy at 9 experimental sites in 8 prefectures. In this, we used new gas analyzer which can measure CH4, CO2 and N2O at once developed by National Institute for Agro-Environmental Sciences (NIAES), Japan. In this report, we show the results in two years of this study. 'Nakaboshi' (mid-season-drainage) is one of cultivation methods in rice paddy that surface water in paddy field is once drained for about 10 days and the field is maintained like upland field to give adequate stress to rice plant for better harvest qualities and yields. Our targeted evaluation was dependencies of Nakaboshi periods lengths and Nakaboshi periods to CH4 emission reduction amounts for total cultivation periods within harvest yield maintained. The longer length of Nakaboshi period was extended, the lesser CH4 emitted even after when Nakaboshi period lasted, as a whole. In some cases, for example in Kagoshima, exceptional phenomena of that significant high emission were

[Exposure degree of important non-target arthropods to Cry2Aa in Bt rice fields].

PubMed

Zhang, Qing-Ling; Li, Yun-He; Hua, Hong-Xia; Yang, Chang-Ju; Wu, Hong-Jin; Peng, Yu-Fa

2013-06-01

Based on the principle of "risk = hazard x exposure", the selected representative nontarget organisms in the assessment of the potential effects of insect-resistant genetically modified (GM) crops on non-target arthropods in laboratory are generally the arthropod species highly exposed to the insecticidal proteins expressed by the GM crops in farmland ecosystem. In order to understand the exposure degree of the important arthropod species to Cry proteins in Bt rice fields, and to select the appropriate non-target arthropods in the risk assessment of insect-resistant GM crops, the enzyme-linked immunosorbent assay (ELISA) was conducted to measure the Cry2Aa protein concentration in the arthropods collected from the cry2Aa rice fields at different rice growth stages. The results showed that there was a significant difference in the Cry2Aa content protein concentration in different arthropod species. Some species did not contain Cry2Aa protein, while some species contained larger amounts of Cry2Aa protein. Relative to the arthropods colleted after rice anthesis, the arthropods colleted in rice anthesis contained relative higher concentrations of Cry2Aa protein, especially for the predacious arthropods. No Cry proteins were detected in parasitic arthropods. This study provided references for the laboratory assessment of the effects of GM rice on nontarget arthropods.

A Study toward the Evaluation of ALOS Images for LAI Estimation in Rice Fields

NASA Astrophysics Data System (ADS)

Sharifi Hashjin, Sh.; Darvishzadeh, R.; Khandan, R.

2013-10-01

For expanding and managing agricultural sources, satellite data have a key role in determining required information about different factors in plants Including Leaf Area Index (LAI).This paper has studied the potential of spectral indices in estimating rice canopy LAI in Amol city as one of the main sources of rice production in Iran. Due to its importance in provision of food and calorie of a major portion of population, rice product was chosen for study. A field campaign was conducted when rice was in the max growth stage (late of June). Also, two satellite images from ALOS-AVNIR-2 were used (simultaneous with conducted field works) to extract and determine vegetation indices. Then the Regression between measured data and vegetation indices, derived from combination of different bands, was evaluated and after that suitable vegetation indices were realized. Finally, statistics and calculations for introduction of a suitable model were presented. After examination of models, the results showed that RDVI and SAVI2, by determination coefficient and RMSE of 0.12-0.59 and 0.24-0.62, have more accuracy in LAI estimation. Results of present study demonstrated the potential of ALOS images, for LAI estimation and their significant role in monitoring and managing the rice plant.

Low uptake affinity cultivars with biochar to tackle Cd-tainted rice--A field study over four rice seasons in Hunan, China.

PubMed

Chen, De; Guo, Hu; Li, Ruiyue; Li, Lianqing; Pan, Genxing; Chang, Andrew; Joseph, Stephen

2016-01-15

Biochar is becoming an environmentally friendly material for remediation of heavy metal contaminated soils and improving food safety. A field trial over four rice seasons was conducted to investigate the use of biochar and low Cd accumulating cultivars on Cd uptake in a heavy metal contaminated soil. Wheat straw derived biochar was applied at 0, 20 and 40 t ha(-1). Two rice cultivars with differing Cd accumulation abilities were selected in each season. The results showed that both biochar and low Cd affinity cultivars significantly reduced rice grain Cd accumulation. Biochar had no significant effect the first season but thereafter consistently reduced rice grain Cd by a maximum of 61, 86 and 57% over the next three seasons. Zn accumulation in the rice grains was not decreased by biochar application, although available soil Zn was sharply reduced (35-91%). Indica conventional rice cultivars had much lower Cd, but higher Zn and lower Cd/Zn ratios in the grain than indica hybrid cultivars. Biochar was more effective for mitigating grain Cd accumulation in low Cd affinity cultivars than in high affinity cultivars. Soil pH was sustainably increased (up to nearly 1 unit) while available Cd significantly decreased by a maximum of 85% after biochar addition. The translocation of Cd from rice roots to shoots was reduced from 20 to 80% by biochar. Low uptake affinity cultivars combined with biochar reduced late rice grain Cd concentration and Cd/Zn ratios by 69-80% and 72-80%, respectively. It indicated that the management of combining biochar and low Cd affinity cultivars should be an efficient way to remediate Cd contaminated rice paddies and reduce health risk associated with consuming rice from these soils. Copyright © 2015 Elsevier B.V. All rights reserved.

Impact of spatial plan on the conversion of Subak rice fields and food security, in Badung and Gianyar Regencies, Bali Province

NASA Astrophysics Data System (ADS)

Lanya, Indayati; Netera Subadiyasa, N.; Ratna Adi, Gst. P.

2018-05-01

Regional Spatial Plan of Bali Province 2009-2029, allocating rice fields can be converted 10% (± 10.800 ha). Over the next 20 years, the conversion of rice field is permitted 540 ha year-1, the real condition in Bali is 800 ha year-1.Research location in Badung and Gianyar Regencies. Visual satellite image interpretation methods, digitization of on-screen, delineation of subak rice field, field survey, superimpose analysis of Spatial Plan (SP) map with rice field map, trough toolbox-analysis tools–overlay-intersect using QGIS, Harvest Index (HI) of cropping pattern in one year. SP has a negative impact on agricultural land resources and food security. Local Regulation (SP), subak rice fields outside the agricultural area licensed to be converted, and food deficits. Regency of Badung, potential land conversion of 3,324.97 ha (34.44%) from 119 subak with paddy field area of 24,184.85 ha. There are 10 subak 100% and 8 subak > 95% can be converted; projected food deficit -115.343 tons of rice by 2020 for HI 2. In Gianyar Regency, potential land conversion 13,021.41 ha (53.51%) of 66 subak with an area of 24184.85 ha; 8 subak 100% and 8 subak with area < 5 ha can be converted; projected food deficit is about -194438 tons of rice in 2040 for HI 2.

Genetics, Physiological Mechanisms and Breeding of Flood-Tolerant Rice (Oryza sativa L.).

PubMed

Singh, Anuradha; Septiningsih, Endang M; Balyan, Harendra S; Singh, Nagendra K; Rai, Vandna

2017-02-01

Flooding of rice fields is a serious problem in the river basins of South and South-East Asia where about 15 Mha of lowland rice cultivation is regularly affected. Flooding creates hypoxic conditions resulting in poor germination and seedling establishment. Flash flooding, where rice plants are completely submerged for 10-15 d during their vegetative stage, causes huge losses. Water stagnation for weeks to months also leads to substantial yield losses when large parts of rice aerial tissues are inundated. The low-yielding traditional varieties and landraces of rice adapted to these flooding conditions have been replaced by flood-sensitive high-yielding rice varieties. The 'FR13A' rice variety and the Submergence 1A (SUB1A) gene were identified for flash flooding and subsequently introgressed to high-yielding rice varieties. The challenge is to find superior alleles of the SUB1A gene, or even new genes that may confer greater tolerance to submergence. Similarly, genes have been identified in tolerant landraces of rice for their ability to survive by rapid stem elongation (SNORKEL1 and SNORKEL2) during deep-water flooding, and for anaerobic germination ability (TPP7). Research on rice genotypes and novel genes that are tolerant to prolonged water stagnation is in progress. These studies will greatly assist in devising more efficient and precise molecular breeding strategies for developing climate-resilient high-yielding rice varieties for flood-prone regions. Here we review the state of our knowledge of flooding tolerance in rice and its application in varietal improvement. © The Author 2017. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oup.com.

Dissipation of the Herbicide Benzobicyclon Hydrolysate in a Model California Rice Field Soil.

PubMed

Williams, Katryn L; Gladfelder, Joshua J; Quigley, Lindsay L; Ball, David B; Tjeerdema, Ronald S

2017-10-25

The herbicide benzobicyclon (BZB; 3-(2-chloro-4-(methylsulfonyl)benzoyl)-2-phenylthiobicyclo[3.2.1]oct-2-en-4-one) has recently been approved for use on California rice fields by the United States Environmental Protection Agency (U.S. EPA). Hydrolysis of BZB rapidly forms the active compound, benzobicyclon hydrolysate (BH), whose fate is currently not well understood. A model California rice soil was used to determine BH soil dissipation. The pK a and aqueous solubility were also determined, as experimental values are not currently available. Sorption data indicate BH does not bind tightly, or irreversibly, with this soil. Flooding resulted in decreased BH loss, indicating anaerobic microbes are less likely to transform BH compared to aerobic microorganisms. Temperature increased dissipation, while autoclaving decreased BH loss. Overall, dissipation was slow regardless of treatment. Further investigation is needed to elucidate the exact routes of loss in soil, though BH is expected to dissipate slowly in flooded rice field soil.

Mapping cropping patterns in irrigated rice fields in West Java: Towards mapping vulnerability to flooding using time-series MODIS imageries

NASA Astrophysics Data System (ADS)

Sianturi, Riswan; Jetten, V. G.; Sartohadi, Junun

2018-04-01

Information on the vulnerability to flooding is vital to understand the potential damages from flood events. A method to determine the vulnerability to flooding in irrigated rice fields using the Enhanced Vegetation Index (EVI) was proposed in this study. In doing so, the time-series EVI derived from time-series 8 day 500 m spatial resolution MODIS imageries (MOD09A1) was used to generate cropping patterns in irrigated rice fields in West Java. Cropping patterns were derived from the spatial distribution and phenology metrics so that it is possible to show the variation of vulnerability in space and time. Vulnerability curves and cropping patterns were used to determine the vulnerability to flooding in irrigated rice fields. Cropping patterns capture the shift in the vulnerability, which may lead to either an increase or decrease of the degree of damage in rice fields of origin and other rice fields. The comparison of rice field areas between MOD09A1 and ALOS PALSAR and MOD09A1 and Agricultural Statistics showed consistent results with R2 = 0.81 and R2 = 0.93, respectively. The estimated and observed DOYs showed RMSEs = 9.21, 9.29, and 9.69 days for the Start of Season (SOS), heading stage, and End of Season (EOS), respectively. Using the method, one can estimate the relative damage provided available information on the flood depth and velocity. The results of the study may support the efforts to reduce the potential damages from flooding in irrigated rice fields.

Potent antioxidant and radical-scavenging activities of traditional Japanese cereal grains.

PubMed

Higashi-Okai, Kiyoka; Ishida, Emi; Nakamura, Yumiko; Fujiwara, Satomi; Okai, Yasuji

2008-12-01

To estimate the preventive potential of Japanese traditional cereals against oxygen radical-related chronic diseases such as cardiovascular diseases and diabetes, antioxidant and radical-scavenging activities in the extracts of five Japanese traditional cereal grains were analyzed by using an assay system of lipid peroxidation and a radical compound, 1,1-diphenyl-2-picrylhydrazyl (DPPH). DPPH radical-scavenging activities in the extracts of Japanese cereal grains were divided into two groups. One group including Japanese sorghum, black rice and red rice showed strong radical-scavenging activities, but the other group including Japanese barnyard millet and foxtail millet did not exhibit significant radical-scavenging activities. The DPPH radical-scavenging activities of these extracts were closely correlated to the contents of phenolic compound in the extracts, but not to the sugar or protein content in the extracts. In contrast, all the methanol and water extracts of the cereal grains caused significant antioxidant activities against hydroperoxide generation in the peroxidation of linoleic acid, in which the water extracts of these cereal grains caused much higher antioxidant activities than the methanol extracts of the same cereals. These results suggest that Japanese traditional cereals contain qualitatively different principles associated with antioxidant and radical-scavenging activities, and possible principles responsible for the antioxidant and radical-scavenging activities in the cereal grains are discussed.

Populations of Rice Grain Bug, Paraeuscosmetus pallicomis, (Hemiptera: Lygaeidae) in Weed-free Paddy Field, Weedy Paddy Field and Paddy Dykes

PubMed Central

Abdullah, Tamrin; Nasruddin, Andi; Agus, Nurariaty

2017-01-01

Research on the populations of rice grain bug Paraeuscosmetus pallicomis Dallas (Hemiptera: Lygaeidae) in paddy field ecosystems was performed with the aim to determine the populations of rice grain bug in weed-free paddy field, weedy paddy field, and paddy dykes. Experiment was carried out in the village of Paccellekang in the district of Patallasang of Gowa Regency in South Sulawesi, Indonesia. Observations were performed during the milky grain stage (85 days after planting), the mature grain stage (105 days after planting), and one day after harvest (115 days after transplanting). Results showed that 85 days after the transplanting, the populations of rice grain bug was significantly higher in the weedy paddy field compared to weed-free field and paddy dykes with total numbers of 1.75, 3.53, and 0.31 insects per 2 hills, respectively. Similarly, 105 days after the transplanting, 2.53, 5.53, and 0.11 insects per hill, respectively. However, one day after the harvest (115 days after transplanting) the number of insects in weed-free field decreased, while in the dykes increased, and the weedy plot still had the highest number of insects per 2 hills. Our results suggested that weeds played an important role in regulating the bug population by providing alternative shelter and foods for the insect. PMID:28890757

Genomic Evolution of Saccharomyces cerevisiae under Chinese Rice Wine Fermentation

PubMed Central

Li, Yudong; Zhang, Weiping; Zheng, Daoqiong; Zhou, Zhan; Yu, Wenwen; Zhang, Lei; Feng, Lifang; Liang, Xinle; Guan, Wenjun; Zhou, Jingwen; Chen, Jian; Lin, Zhenguo

2014-01-01

Rice wine fermentation represents a unique environment for the evolution of the budding yeast, Saccharomyces cerevisiae. To understand how the selection pressure shaped the yeast genome and gene regulation, we determined the genome sequence and transcriptome of a S. cerevisiae strain YHJ7 isolated from Chinese rice wine (Huangjiu), a popular traditional alcoholic beverage in China. By comparing the genome of YHJ7 to the lab strain S288c, a Japanese sake strain K7, and a Chinese industrial bioethanol strain YJSH1, we identified many genomic sequence and structural variations in YHJ7, which are mainly located in subtelomeric regions, suggesting that these regions play an important role in genomic evolution between strains. In addition, our comparative transcriptome analysis between YHJ7 and S288c revealed a set of differentially expressed genes, including those involved in glucose transport (e.g., HXT2, HXT7) and oxidoredutase activity (e.g., AAD10, ADH7). Interestingly, many of these genomic and transcriptional variations are directly or indirectly associated with the adaptation of YHJ7 strain to its specific niches. Our molecular evolution analysis suggested that Japanese sake strains (K7/UC5) were derived from Chinese rice wine strains (YHJ7) at least approximately 2,300 years ago, providing the first molecular evidence elucidating the origin of Japanese sake strains. Our results depict interesting insights regarding the evolution of yeast during rice wine fermentation, and provided a valuable resource for genetic engineering to improve industrial wine-making strains. PMID:25212861

Preferential Association of Endophytic Bradyrhizobia with Different Rice Cultivars and Its Implications for Rice Endophyte Evolution

PubMed Central

Piromyou, Pongdet; Greetatorn, Teerana; Teamtisong, Kamonluck; Okubo, Takashi; Shinoda, Ryo; Nuntakij, Achara; Tittabutr, Panlada; Boonkerd, Nantakorn

2015-01-01

Plant colonization by bradyrhizobia is found not only in leguminous plants but also in nonleguminous species such as rice. To understand the evolution of the endophytic symbiosis of bradyrhizobia, the effect of the ecosystems of rice plantations on their associations was investigated. Samples were collected from various rice (Oryza sativa) tissues and crop rotational systems. The rice endophytic bradyrhizobia were isolated on the basis of oligotrophic properties, selective medium, and nodulation on siratro (Macroptilium atropurpureum). Six bradyrhizobial strains were obtained exclusively from rice grown in a crop rotational system. The isolates were separated into photosynthetic bradyrhizobia (PB) and nonphotosynthetic bradyrhizobia (non-PB). Thai bradyrhizobial strains promoted rice growth of Thai rice cultivars better than the Japanese bradyrhizobial strains. This implies that the rice cultivars possess characteristics that govern rice-bacterium associations. To examine whether leguminous plants in a rice plantation system support the persistence of rice endophytic bradyrhizobia, isolates were tested for legume nodulation. All PB strains formed symbioses with Aeschynomene indica and Aeschynomene evenia. On the other hand, non-PB strains were able to nodulate Aeschynomene americana, Vigna radiata, and M. atropurpureum but unable to nodulate either A. indica or A. evenia. Interestingly, the nodABC genes of all of these bradyrhizobial strains seem to exhibit low levels of similarity to those of Bradyrhizobium diazoefficiens USDA110 and Bradyrhizobium sp. strain ORS285. From these results, we discuss the evolution of the plant-bradyrhizobium association, including nonlegumes, in terms of photosynthetic lifestyle and nod-independent interactions. PMID:25710371

Biofortification of rice with the essential amino acid lysine: molecular characterization, nutritional evaluation, and field performance

PubMed Central

Yang, Qing-qing; Zhang, Chang-quan; Chan, Man-ling; Zhao, Dong-sheng; Chen, Jin-zhu; Wang, Qing; Li, Qian-feng; Yu, Heng-xiu; Gu, Ming-hong; Sun, Samuel Sai-ming; Liu, Qiao-quan

2016-01-01

Rice (Oryza sativa L.), a major staple crop worldwide, has limited levels of the essential amino acid lysine. We previously produced engineered rice with increased lysine content by expressing bacterial aspartate kinase and dihydrodipicolinate synthase and inhibiting rice lysine ketoglutarate reductase/saccharopine dehydrogenase activity. However, the grain quality, field performance, and integration patterns of the transgenes in these lysine-enriched lines remain unclear. In the present study, we selected several elite transgenic lines with endosperm-specific or constitutive regulation of the above key enzymes but lacking the selectable marker gene. All target transgenes were integrated into the intragenic region in the rice genome. Two pyramid transgenic lines (High Free Lysine; HFL1 and HFL2) with free lysine levels in seeds up to 25-fold that of wild type were obtained via a combination of the above two transgenic events. We observed a dramatic increase in total free amino acids and a slight increase in total protein content in both pyramid lines. Moreover, the general physicochemical properties were improved in pyramid transgenic rice, but the starch composition was not affected. Field trials indicated that the growth of HFL transgenic rice was normal, except for a slight difference in plant height and grain colour. Taken together, these findings will be useful for the potential commercialization of high-lysine transgenic rice. PMID:27252467

Effects of raising frogs and putting pest-killing lamps in paddy fields on the prevention of rice pests and diseases

NASA Astrophysics Data System (ADS)

Teng, Qing; Hu, Xue-Feng; Luo, Fan; Cao, Ming-Yang

2014-05-01

Frogs in paddy fields become less and less due to applying large amounts of pesticides and human hunting for a long time, which causes the aggravation of rice pests and diseases. A field experiment was carried out in the suburb of Shanghai to study the effects of artificially raising frogs and putting frequency oscillation pest-killing lamps in paddy fields on the prevention of rice pests and diseases. The field experiment includes three treatments. Treatment I: 150 frogs, each 20 g in weight, per 100 m2 were put in the fields; Treatment II: a frequency oscillation pest-killing lamp was put in the fields; Treatment III: no frogs and pest-killing lamps were put in the fields. All the experimental fields were operated based on the organic faming system. The amount of organic manure, 7500 kg/hm2, was applied to the fields as base fertilizer before sowing in early June, 2013. No any chemical fertilizers and pesticides were used during the entire period of rice growth. Each treatment is in triplicate and each plot is 67 m2 in area. The results are as follows: (1) During the entire growth period, the incidences of rice pests and diseases with Treatment I and II are significantly lower than those with CK (Treatment III). The incidence of chilo suppressalis with Treatment I, II and III is 0, 0.46% and 1.69%, respectively; that of cnaphalocrocis medinalis is 7.67%, 6.62% and 10.10%, respectively; that of rice sheath blight is 0, 11.11% and 5.43%, respectively; that of rice planthopper is 4.25 per hill, 5.75 per hill and 11 per hill, respectively. (2) The grain yield of the three treatments is significantly different. That of Treatment I, II and III is 5157.73 kg/hm2, 4761.60 kg/hm2 and 3645.14kg/hm2 on average, respectively. (3) Affected by frog activities, the contents of NH4-N, available P and available K in the soil with Treatment I are significantly raised. All the above suggest that artificially raising frogs in paddy fields could effectively prevent rice pests and

Electricity Generation in Microbial Fuel Cell (MFC) by Bacterium Isolated from Rice Paddy Field Soil

NASA Astrophysics Data System (ADS)

Fakhirruddin, Fakhriah; Amid, Azura; Salim, Wan Wardatul Amani Wan; Suhaida Azmi, Azlin

2018-03-01

Microbial fuel cell (MFC) is an alternative approach in generating renewable energy by utilising bacteria that will oxidize organic or inorganic substrates, producing electrons yielded as electrical energy. Different species of exoelectrogenic bacteria capable of generating significant amount of electricity in MFC has been identified, using various organic compounds for fuel. Soil sample taken from rice paddy field is proven to contain exoelectrogenic bacteria, thus electricity generation using mixed culture originally found in the soil, and pure culture isolated from the soil is studied. This research will isolate the exoelectrogenic bacterial species in the rice paddy field soil responsible for energy generation. Growth of bacteria isolated from the MFC is observed by measuring the optical density (OD), cell density weight (CDW) and viable cell count. Mixed bacterial species found in paddy field soil generates maximum power of 77.62 μW and 0.70 mA of current. In addition, the research also shows that the pure bacterium in rice paddy field soil can produce maximum power and current at 51.32 μW and 0.28 mA respectively.

Arsenic in soil and irrigation water affects arsenic uptake by rice: complementary insights from field and pot studies.

PubMed

Dittmar, Jessica; Voegelin, Andreas; Maurer, Felix; Roberts, Linda C; Hug, Stephan J; Saha, Ganesh C; Ali, M Ashraf; Badruzzaman, A Borhan M; Kretzschmar, Ruben

2010-12-01

Groundwater rich in arsenic (As) is extensively used for dry season boro rice cultivation in Bangladesh, leading to long-term As accumulation in soils. This may result in increasing levels of As in rice straw and grain, and eventually, in decreasing rice yields due to As phytotoxicity. In this study, we investigated the As contents of rice straw and grain over three consecutive harvest seasons (2005-2007) in a paddy field in Munshiganj, Bangladesh, which exhibits a documented gradient in soil As caused by annual irrigation with As-rich groundwater since the early 1990s. The field data revealed that straw and grain As concentrations were elevated in the field and highest near the irrigation water inlet, where As concentrations in both soil and irrigation water were highest. Additionally, a pot experiment with soils and rice seeds from the field site was carried out in which soil and irrigation water As were varied in a full factorial design. The results suggested that both soil As accumulated in previous years and As freshly introduced with irrigation water influence As uptake during rice growth. At similar soil As contents, plants grown in pots exhibited similar grain and straw As contents as plants grown in the field. This suggested that the results from pot experiments performed at higher soil As levels can be used to assess the effect of continuing soil As accumulation on As content and yield of rice. On the basis of a recently published scenario of long-term As accumulation at the study site, we estimate that, under unchanged irrigation practice, average grain As concentrations will increase from currently ∼0.15 mg As kg(-1) to 0.25-0.58 mg As kg(-1) by the year 2050. This translates to a 1.5-3.8 times higher As intake by the local population via rice, possibly exceeding the provisional tolerable As intake value defined by FAO/WHO.

Rice microstructure

USDA-ARS?s Scientific Manuscript database

An understanding of plant structure is desirable to obtain a clear idea of the overall impact of a crop. A mature rice plant consists of leafy components (left in the field post-harvest) and paddy rice (collected). The rice plant is supported by a hollow stem (culm) with leaf sheaths attached to nod...

Paddy-field contamination with 134Cs and 137Cs due to Fukushima Dai-ichi Nuclear Power Plant accident and soil-to-rice transfer coefficients.

PubMed

Endo, Satoru; Kajimoto, Tsuyoshi; Shizuma, Kiyoshi

2013-02-01

The transfer coefficient (TF) from soil to rice plants of (134)Cs and (137)Cs in the form of radioactive deposition from the Fukushima Dai-ichi Nuclear Power Plant (FDNPP) accident in March 2011 was investigated in three rice paddy fields in Minami-Soma City. Rice crops were planted in the following May and harvested at the end of September. Soil cores of 30-cm depth were sampled from rice-planted paddy fields to measure (134)Cs and (137)Cs radioactivity at 5-cm intervals. (134)Cs and (137)Cs radioactivity was also measured in rice ears (rice with chaff), straws and roots. The rice ears were subdivided into chaff, brown rice, polished rice and rice bran, and the (134)Cs and (137)Cs radioactivity concentration of each plant part was measured to calculate the respective TF from the soil. The TF of roots was highest at 0.48 ± 0.10 in the field where the (40)K concentration in the soil core was relatively low, in comparison with TF values of 0.31 and 0.38 in other fields. Similar trends could be found for the TF of whole rice plants, excluding roots. The TF of rice ears was relatively low at 0.019-0.026. The TF of chaff, rice bran, brown rice and polished rice was estimated to be 0.049, 0.10-0.16, 0.013-0.017 and 0.005-0.013, respectively. Copyright © 2012 Elsevier Ltd. All rights reserved.

Structure and function of the methanogenic microbial communities in Uruguayan soils shifted between pasture and irrigated rice fields.

PubMed

Scavino, Ana Fernandez; Ji, Yang; Pump, Judith; Klose, Melanie; Claus, Peter; Conrad, Ralf

2013-09-01

Irrigated rice fields in Uruguay are temporarily established on soils used as cattle pastures. Typically, 4 years of cattle pasture are alternated with 2 years of irrigated rice cultivation. Thus, oxic upland conditions are rotated with seasonally anoxic wetland conditions. Only the latter conditions are suitable for the production of CH4 from anaerobic degradation of organic matter. We studied soil from a permanent pasture as well as soils from different years of the pasture-rice rotation hypothesizing that activity and structure of the bacterial and archaeal communities involved in production of CH4 change systematically with the duration of either oxic or anoxic conditions. Soil samples were taken from drained fields, air-dried and used for the experiments. Indeed, methanogenic archaeal gene copy numbers (16S rRNA, mcrA) were lower in soil from the permanent pasture than from the pasture-rice alternation fields, but within the latter, there was no significant difference. Methane production started to accumulate after 16 days and 7 days of anoxic incubation in soil from the permanent pasture and the pasture-rice alternation fields respectively. Then, CH4 production rates were slightly higher in the soils used for pasture than for rice production. Analysis of δ(13) C in CH4, CO2 and acetate in the presence and absence of methyl fluoride, an inhibitor of aceticlastic methanogenesis, indicated that CH4 was mainly (58-75%) produced from acetate, except in the permanent pasture soil (42%). Terminal restriction fragment length polymorphism (T-RFLP) of archaeal 16S rRNA genes showed no difference among the soils from the pasture-rice alternation fields with Methanocellaceae and Methanosarcinaceae as the main groups of methanogens, but in the permanent pasture soil, Methanocellaceae were relatively less abundant. T-RFLP analysis of bacterial 16S rRNA genes allowed the distinction of permanent pasture and fields from the pasture-rice rotation, but nevertheless with a

Dynamical roguing model for controlling the spread of tungro virus via Nephotettix Virescens in a rice field

NASA Astrophysics Data System (ADS)

Blas, Nikki; David, Guido

2017-10-01

Rice tungro disease is described as a cancer due to its major impact on the livelihood of farmers and the difficulty of controlling it. Tungro is a semi-persistent virus transmitted by green leafhoppers called Nephotettix Virescens. In this paper, we presented a compartmental plant-vector model of the Nephotettix Virescens - rice plant interaction based on a system of ordinary differential equations to simulate the effects of roguing in controlling the spread of Tungro virus in a model rice field of susceptible rice variety (Taichung Native 1).

Agronomic and environmental aspects of diazotrophic bacteria in irrigated rice fields

USDA-ARS?s Scientific Manuscript database

This article provides an overview of the free-living and plant-associated nitrogen-fixing bacterial communities in irrigated rice fields, with a focus on describing the drivers affecting community assemblages in this soil-water-plant-atmosphere system. Theoretical and technical advances in non-legu...

Richness and density of aquatic benthic macroinvertebrates after exposure to fungicides and insecticides in rice paddy fields.

PubMed

Wandscheer, Alana C D; Marchesan, Enio; Santos, Sandro; Zanella, Renato; Silva, Marília F; Londero, Guilherme P; Donato, Gabriel

2017-01-01

The objective of this study was to verify the richness and density of aquatic benthic macroinvertebrates after exposure to fungicides and insecticides of the rice paddy fields. In the crop seasons of 2012/13 and 2013/14, field experiments were performed, which consisted of single-dose applications of the fungicides trifloxystrobin + tebuconazole and tricyclazole, and the insecticides lambda-cyhalothrin + thiamethoxam and diflubenzuron, in 10 m2 experimental plots, over rice plants in the R3 stage. Control plots with and without rice plants were maintained in order to simulate a natural environment. Soil samples were collected during rice cultivation for assessment of the macroinvertebrate fauna. Chemical-physical parameters assessed in the experiments included temperature, pH and oxygen dissolved in the water and pesticide persistence in the water and in the soil. The application of a single dose of the pesticides and fungicides in the recommended period does not cause significant negative effects over the richness and density of the macroinvertebrates. Tebuconazole, tricyclazole and thiamethoxam showed high persistence in the irrigation water of rice paddy fields. Thus, the doses and number of applications of these products in crops should be carefully handled in order to avoid contamination of the environment.

Wolbachia infection complexity among insects in the tropical rice-field community.

PubMed

Kittayapong, P; Jamnongluk, W; Thipaksorn, A; Milne, J R; Sindhusake, C

2003-04-01

Wolbachia are a group of intracellular bacteria that cause reproductive alterations in their arthropod hosts. Widely discordant host and Wolbachia phylogenies indicate that horizontal transmission of these bacteria among species sometimes occurs. A likely means of horizontal transfer is through the feeding relations of organisms within communities. Feeding interactions among insects within the rice-field insect community have been well documented in the past. Here, we present the results of a polymerase chain reaction-based survey and phylogenetic analysis of Wolbachia strains in the rice-field insect community of Thailand. Our field survey indicated that 49 of 209 (23.4%) rice-field insect species were infected with Wolbachia. Of the 49 infected species, 27 were members of two feeding complexes: (i) a group of 13 hoppers preyed on by 2 mirid species and parasitized by a fly species, and (ii) 2 lepidopteran pests parasitized by 9 wasp species. Wolbachia strains found in three hoppers, Recilia dorsalis, Nephotettix malayanus and Nisia nervosa, the two mirid predators, Cyrtorhinus lividipennis and Tytthus chinensis, and the fly parasitoid, Tomosvaryella subvirescens, were all in the same Wolbachia clade. In the second complex, the two lepidopteran pests, Cnaphalocrocis medinalis and Scirpophaga incertulas, were both infected with Wolbachia from the same clade, as was the parasitoid Tropobracon schoenobii. However, none of the other infected parasitoid species in this feeding complex was infected by Wolbachia from this clade. Mean (+/- SD) genetic distance of Wolbachia wsp sequences among interacting species pairs of the hopper feeding complex (0.118 +/- 0.091 nucleotide sequence differences), but not for the other two complexes, was significantly smaller than that between noninteracting species pairs (0.162 +/- 0.079 nucleotide sequence differences). Our results suggest that some feeding complexes, such as the hopper complex described here, could be an important

Growth and Survival of Baldcypress Planted in an Old Rice Field of Coastal South Carolina

Treesearch

William H. Conner; L. Wayne Inabinette; Mehmet Ozalp

2004-01-01

Vast acreages of baldcypress [Taxodium distichum (L.) Rich.] swampland in coastal South Carolina were cleared for rice production starting in the late 1600s. When rice cultivation ended in the late 1800s, many cultivated areas became marshlands. Other fields failed to return to forest unless they were planted. In one such area, nine acres were...

Rapid Measurement of Soil Carbon in Rice Paddy Field of Lombok Island Indonesia Using Near Infrared Technology

NASA Astrophysics Data System (ADS)

Kusumo, B. H.; Sukartono, S.; Bustan, B.

2018-02-01

Measuring soil organic carbon (C) using conventional analysis is tedious procedure, time consuming and expensive. It is needed simple procedure which is cheap and saves time. Near infrared technology offers rapid procedure as it works based on the soil spectral reflectance and without any chemicals. The aim of this research is to test whether this technology able to rapidly measure soil organic C in rice paddy field. Soil samples were collected from rice paddy field of Lombok Island Indonesia, and the coordinates of the samples were recorded. Parts of the samples were analysed using conventional analysis (Walkley and Black) and some other parts were scanned using near infrared spectroscopy (NIRS) for soil spectral collection. Partial Least Square Regression (PLSR) Models were developed using data of soil C analysed using conventional analysis and data from soil spectral reflectance. The models were moderately successful to measure soil C in rice paddy field of Lombok Island. This shows that the NIR technology can be further used to monitor the C change in rice paddy soil.

Water consumption, grain yield, and water productivity in response to field water management in double rice systems in China.

PubMed

Wu, Xiao Hong; Wang, Wei; Yin, Chun Mei; Hou, Hai Jun; Xie, Ke Jun; Xie, Xiao Li

2017-01-01

Rice cultivation has been challenged by increasing food demand and water scarcity. We examined the responses of water use, grain yield, and water productivity to various modes of field water managements in Chinese double rice systems. Four treatments were studied in a long-term field experiment (1998-2015): continuous flooding (CF), flooding-midseason drying-flooding (F-D-F), flooding-midseason drying-intermittent irrigation without obvious standing water (F-D-S), and flooding-rain-fed (F-RF). The average precipitation was 483 mm in early-rice season and 397 mm in late-rice season. The irrigated water for CF, F-D-F, F-D-S, and F-RF, respectively, was 263, 340, 279, and 170 mm in early-rice season, and 484, 528, 422, and 206 mm in late-rice season. Grain yield for CF, F-D-F, F-D-S, and F-RF, respectively, was 4,722, 4,597, 4,479, and 4,232 kgha-1 in early-rice season, and 5,420, 5,402, 5,366, and 4,498 kgha-1 in late-rice season. Compared with CF, F-D-F consumed more irrigated water, which still decreased grain yield, leading to a decrease in water productivity by 25% in early-rice season and by 8% in late-rice season. Compared with F-D-F, F-D-S saved much irrigated water with a small yield reduction, leading to an increase in water productivity by 22% in early-rice season and by 26% in late-rice season. The results indicate that CF is best for early-rice and FDS is best for late-rice in terms of grain yield and water productivity.

Genomic evolution of Saccharomyces cerevisiae under Chinese rice wine fermentation.

PubMed

Li, Yudong; Zhang, Weiping; Zheng, Daoqiong; Zhou, Zhan; Yu, Wenwen; Zhang, Lei; Feng, Lifang; Liang, Xinle; Guan, Wenjun; Zhou, Jingwen; Chen, Jian; Lin, Zhenguo

2014-09-10

Rice wine fermentation represents a unique environment for the evolution of the budding yeast, Saccharomyces cerevisiae. To understand how the selection pressure shaped the yeast genome and gene regulation, we determined the genome sequence and transcriptome of a S. cerevisiae strain YHJ7 isolated from Chinese rice wine (Huangjiu), a popular traditional alcoholic beverage in China. By comparing the genome of YHJ7 to the lab strain S288c, a Japanese sake strain K7, and a Chinese industrial bioethanol strain YJSH1, we identified many genomic sequence and structural variations in YHJ7, which are mainly located in subtelomeric regions, suggesting that these regions play an important role in genomic evolution between strains. In addition, our comparative transcriptome analysis between YHJ7 and S288c revealed a set of differentially expressed genes, including those involved in glucose transport (e.g., HXT2, HXT7) and oxidoredutase activity (e.g., AAD10, ADH7). Interestingly, many of these genomic and transcriptional variations are directly or indirectly associated with the adaptation of YHJ7 strain to its specific niches. Our molecular evolution analysis suggested that Japanese sake strains (K7/UC5) were derived from Chinese rice wine strains (YHJ7) at least approximately 2,300 years ago, providing the first molecular evidence elucidating the origin of Japanese sake strains. Our results depict interesting insights regarding the evolution of yeast during rice wine fermentation, and provided a valuable resource for genetic engineering to improve industrial wine-making strains. © The Author(s) 2014. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.

Direct and reverse pollen-mediated gene flow between GM rice and red rice weed

PubMed Central

Serrat, X.; Esteban, R.; Peñas, G.; Català, M. M.; Melé, E.; Messeguer, J.

2013-01-01

Potential risks of genetically modified (GM) crops must be identified before their commercialization, as happens with all new technologies. One of the major concerns is the proper risk assessment of adventitious presence of transgenic material in rice fields due to cross-pollination. Several studies have been conducted in order to quantify pollen-mediated gene flow from transgenic rice (Oryza sativa) to both conventional rice and red rice weed (O. sativa f. spontanea) under field conditions. Some of these studies reported GM pollen-donor rice transferring GM traits to red rice. However, gene flow also occurs in the opposite direction, in a phenomenon that we have called reverse gene flow, resulting in transgenic seeds that have incorporated the traits of wild red rice. We quantified reverse gene flow using material from two field trials. A molecular analysis based on amplified fragment length polymorphisms was carried out, being complemented with a phenotypic identification of red rice traits. In both field trials, the reverse gene flow detected was greater than the direct gene flow. The rate of direct gene flow varied according to the relative proportions of the donor (GM rice) and receptor (red rice) plants and was influenced by wind direction. The ecological impact of reverse gene flow is limited in comparison with that of direct gene flow because non-shattered and non-dormant seeds would be obtained in the first generation. Hybrid seed would remain in the spike and therefore most of it would be removed during harvesting. Nevertheless, this phenomenon must be considered in fields used for elite seed production and in developing countries where farmers often keep some seed for planting the following year. In these cases, there is a higher risk of GM red rice weed infestation increasing from year to year and therefore a proper monitoring plan needs to be established.

Distribution and identification of proteolytic Bacillus spp. in paddy field soil under rice cultivation.

PubMed

Watanabe, K; Hayano, K

1993-07-01

Proteolytic bacteria in paddy field soils under rice cultivation were characterized and enumerated using azocoll agar plates. Bacillus spp. were the proteolytic bacteria that were most frequently present, comprising 59% of the isolates. They were always the numerically dominant proteolytic bacteria isolated from three kinds of fertilizer treatments (yearly application of rice-straw compost and chemical fertilizer, yearly application of chemical fertilizer, and no fertilizer application) and at three different stages of rice development (vegetative growth stage, maximal tillering stage, and harvest stage). Of the 411 proteolytic bacteria isolated, 124 isolates had stronger proteolytic activity than others on the basis of gelatin liquefaction tests and most of them were Bacillus spp. (100% in 1989 and 92.4% in 1991). Bacillus subtilis and Bacillus cereus were the main bacteria of this group and Bacillus mycoides, Bacillus licheniformis, and Bacillus megaterium were also present. We conclude that these Bacillus spp. are the primary source of soil protease in these paddy fields.

Confirming and identifying new loci for rice blast disease resistance using magnaporthe oryzae field isolates in the US

USDA-ARS?s Scientific Manuscript database

Quantitative trait loci (QTL) in rice play important roles in controlling rice blast disease. In the present study, 10 field isolates of the races IA1, IB1, IB17, and IC1 of U.S. rice blast fungus Magnaporthe oryzae collected in 1996 and 2009 were used to identify blast resistance QTL with a recombi...

Field investigation to assess nutrient emission from paddy field to surface water in river catchment

NASA Astrophysics Data System (ADS)

Kogure, Kanami; Aichi, Masaatsu; Zessner, Matthias

2015-04-01

In order to maintain good river environment, it is remarkably important to understand and to control nutrient behavior such as Nitrogen and Phosphorus. Our former research dealing with nutrient emission analysis in the Tone River basin area in Japan, in addition to urban and industrial waste water, nutrient emission from agricultural activity is dominant pollution source into the river system. Japanese style agriculture produces large amount of rice and paddy field occupies large areas in Japanese river basin areas. While paddy field can deteriorate river water quality by outflow of fertilizer, it is also suggested that paddy field has water purification function. As we carried out investigation in the Tone River Basin area, data were obtained which dissolved nitrogen concentration is lower in discharging water from paddy field than inflowing water into the field. Regarding to nutrient emission impact from paddy field, sufficient data are required to discuss quantitatively seasonal change of material behavior including flooding season and dry season, difference of climate condition, soil type, and rice species, to evaluate year round comprehensive impact from paddy field to the river system. In this research, field survey in paddy field and data collection relating rice production were carried out as a preliminary investigation to assess how Japanese style paddy field contributes year round on surface water quality. Study sites are three paddy fields located in upper reach of the Tone River basin area. The fields are flooded from June to September. In 2014, field investigations were carried out three times in flooding period and twice in dry period. To understand characteristics of each paddy field and seasonal tendency accompanying weather of agricultural event, short term investigations were conducted and we prepare for further long term investigation. Each study site has irrigation water inflow and outflow. Two sites have tile drainage system under the field and

Carbon dioxide emissions and energy balance closure before, during, and after biomass burning in mid-South rice fields

NASA Astrophysics Data System (ADS)

Fong, B.; Adviento-Borbe, A.; Reba, M. L.; Runkle, B.; Suvocarev, K.

2017-12-01

Biomass burning or field burning is a crop management practice that removes rice straw, reduces tillage, controls pests and releases nutrients for the next cropping season. Current field burning emissions are not included in agricultural field annual emissions largely because of the lack of studies, especially on the field scale. Field burning measurements are important for greenhouse gas emission inventories and quantifying the annual carbon footprint of rice. Paired eddy covariance systems were used to measure energy balance, CO2 fluxes, and H2O fluxes in mid-South US rice fields (total area of 25 ha) before, during and after biomass burning for 20 days after harvest. During the biomass burning, air temperatures increased 29°C, while ambient CO2 concentration increased from 402 to 16,567 ppm and H2O concentrations increased from 18.73 to 25.62 ppt. For the burning period, 67-86 kg CO2 ha-1 period-1 was emitted calculated by integrating fluxes over the biomass burning event. However, the estimated emission using aboveground biomass and combustion factors was calculated as 11,733 kg CO2 ha-1 period-1. Part of the difference could be attributed to sensor sensitivity decreasing 80% during burning for two minutes due to smoke. Net ecosystem exchange (NEE) increased by a factor of two, 1.14 before burning to 2.44 μmol m-2 s-1 possibly due to greater reduction of plant material and photosynthesis following burning. This study highlights the contribution of rice straw burning to total CO2 emissions from rice production.

Exposure to pesticide mixtures and DNA damage among rice field workers.

PubMed

Varona-Uribe, Marcela Eugenia; Torres-Rey, Carlos H; Díaz-Criollo, Sonia; Palma-Parra, Ruth Marien; Narváez, Diana María; Carmona, Sandra Patricia; Briceño, Leonardo; Idrovo, Alvaro J

2016-01-01

This study describes the use of pesticides mixtures and their potential association with comet assay results in 223 rice field workers in Colombia. Thirty-one pesticides were quantified in blood, serum, and urine (15 organochlorines, 10 organophosphorus, 5 carbamates, and ethylenethiourea), and the comet assay was performed. Twenty-four (77.42%) pesticides were present in the workers. The use of the maximum-likelihood factor analysis identified 8 different mixtures. Afterwards, robust regressions were used to explore associations between the factors identified and the comet assay. Two groups of mixtures--α-benzene hexachloride (α-BHC), hexachlorobenzene (HCB), and β-BHC (β: 1.21, 95% confidence interval [CI]: 0.33-2.10) and pirimiphos-methyl, malathion, bromophos-methyl, and bromophos-ethyl (β: 11.97, 95% CI: 2.34-21.60)--were associated with a higher percentage of DNA damage and comet tail length, respectively. The findings suggest that exposure to pesticides varies greatly among rice field workers.

Microbial electricity generation in rice paddy fields: recent advances and perspectives in rhizosphere microbial fuel cells.

PubMed

Kouzuma, Atsushi; Kaku, Nobuo; Watanabe, Kazuya

2014-12-01

Microbial fuel cells (MFCs) are devices that use living microbes for the conversion of organic matter into electricity. MFC systems can be applied to the generation of electricity at water/sediment interfaces in the environment, such as bay areas, wetlands, and rice paddy fields. Using these systems, electricity generation in paddy fields as high as ∼80 mW m(-2) (based on the projected anode area) has been demonstrated, and evidence suggests that rhizosphere microbes preferentially utilize organic exudates from rice roots for generating electricity. Phylogenetic and metagenomic analyses have been conducted to identify the microbial species and catabolic pathways that are involved in the conversion of root exudates into electricity, suggesting the importance of syntrophic interactions. In parallel, pot cultures of rice and other aquatic plants have been used for rhizosphere MFC experiments under controlled laboratory conditions. The findings from these studies have demonstrated the potential of electricity generation for mitigating methane emission from the rhizosphere. Notably, however, the presence of large amounts of organics in the rhizosphere drastically reduces the effect of electricity generation on methane production. Further studies are necessary to evaluate the potential of these systems for mitigating methane emission from rice paddy fields. We suggest that paddy-field MFCs represent a promising approach for harvesting latent energy of the natural world.

Proteomic changes in rice leaves grown under open field high temperature stress conditions.

PubMed

Das, Smruti; Krishnan, P; Mishra, Vagish; Kumar, Ritesh; Ramakrishnan, B; Singh, N K

2015-11-01

The interactive effect of temperature with other climatic and soil factors has profound influences on the growth and development of rice. The responses of rice to high temperatures under field conditions are more important than those under the controlled conditions. To understand the genes associated with high temperature stress response in general and tolerance in particular, the expression of all those genes associated with adaptation and tolerance in rice requires proteomic analysis. High temperature stress-tolerant cv. N22 was subjected to 28/18 °C (control) and 42/32 °C (high temperature stress) at flowering stage. The plants were grown in the field under the free air temperature increment condition. The proteomic changes in rice leaves due to high temperature stress were discussed. The proteomes of leaves had about 3000 protein spots, reproducibly detected on 2-dimensional electrophoretic gels with 573 proteins differentially expressed between the control and the high temperature treatments. Putative physiological functions suggested five categories such as growth (15.4%), heat shock proteins (7.7%), regulatory proteins (26.9%), redox homeostasis proteins (11.5%) and energy and metabolism (38.5%) related proteins. The results of the present study suggest that cv. N22, an agronomically recognized temperature tolerant rice cultivar copes with high temperature stress in a complex manner. Several functional proteins play important roles in its responses. The predicted climate change events necessitate more studies using this cultivar under different simulated ecological conditions to identify proteomic changes and the associated genes to be used as biomarkers and to gain a better understanding on the biochemical pathways involved in tolerance.

Green-tuff landslide areas are beneficial for rice nutrition in Japan.

PubMed

Tazaki, Kazue

2006-12-01

Japanese Islands are covered with weathered volcanic rocks and soils. Terraced rice field are located in green-tuff areas which are very fertile but where landslides occur associated to strong earthquakes. The Xray diffraction and X-ray fluorescence analyses of the soils in landslide area identified predominant smectite and Mg, Al, Si, K, Ti, Mn and Fe are main components. The rice leaf showed that S, Cl, K and Ca play important roles for nutrients in the area. Drainpipe systems have set up in the green- tuff areas to reduce the risks of landslides. Reddish brown microbial mats inhabited bacteria and diatom in the drainpipe outlets. The microbial mats are rich in Fe and PO4(3-). The iron bacteria in the ground water have a high metabolic rate suggesting that the weathering materials were produced by not only physical and chemical influence but also by microorganism. Many microorganisms attach to mineral surfaces and show their high impact in the water mineral chemistry in the landslide area. Bacteria in the green-tuff over landslide area play important roles for sustainable agriculture including rice nutrition.

Occurrence of Aspergillus section Flavi and aflatoxins in Brazilian rice: From field to market.

PubMed

Katsurayama, Aline M; Martins, Ligia M; Iamanaka, Beatriz T; Fungaro, Maria Helena P; Silva, Josué J; Frisvad, Jens C; Pitt, John I; Taniwaki, Marta H

2018-02-02

The guarantee of the high quality of rice is of utmost importance because any toxic contaminant may affect consumer health, especially in countries such as Brazil where rice is part of the daily diet. A total of 187 rice samples, from field, processing and market from two different production systems, wetland from the state of Rio Grande do Sul, dryland, from the state of Maranhão and market samples from the state of São Paulo, were analyzed for fungi belonging to Aspergillus section Flavi and the presence of aflatoxins. Twenty-three soil samples from wetland and dryland were also analyzed. A total of 383 Aspergillus section Flavi strains were isolated from rice and soil samples. Using a polyphasic approach, with phenotypic (morphology and extrolite profiles) and molecular data (beta-tubulin gene sequences), five species were identified: A. flavus, A. caelatus, A. novoparasiticus, A. arachidicola and A. pseudocaelatus. This is the first report of these last three species from rice and rice plantation soil. Only seven (17%) of the A. flavus isolates produced type B aflatoxins, but 95% produced kojic acid and 69% cyclopiazonic acid. Less than 14% of the rice samples were contaminated with aflatoxins, but two of the market samples were well above the maximum tolerable limit (5μg/kg), established by the Brazilian National Health Surveillance Agency. Copyright © 2017 Elsevier B.V. All rights reserved.

Spatial distribution and temporal variability of arsenic in irrigated rice fields in Bangladesh. 2. Paddy soil.

PubMed

Dittmar, Jessica; Voegelin, Andreas; Roberts, Linda C; Hug, Stephan J; Saha, Ganesh C; Ali, M Ashraf; Badruzzaman, A Borhan M; Kretzschmar, Ruben

2007-09-01

Arsenic-rich groundwater from shallow tube wells is widely used for the irrigation of boro rice in Bangladesh and West Bengal. In the long term this may lead to the accumulation of As in paddy soils and potentially have adverse effects on rice yield and quality. In the companion article in this issue, we have shown that As input into paddy fields with irrigation water is laterally heterogeneous. To assess the potential for As accumulation in soil, we investigated the lateral and vertical distribution of As in rice field soils near Sreenagar (Munshiganj, Bangladesh) and its changes over a 1 year cycle of irrigation and monsoon flooding. At the study site, 18 paddy fields are irrigated with water from a shallow tube well containing 397 +/- 7 microg L(-1) As. The analysis of soil samples collected before irrigation in December 2004 showed that soil As concentrations in paddy fields did not depend on the length of the irrigation channel between well and field inlet. Within individual fields, however, soil As contents decreased with increasing distance to the water inlet, leading to highly variable topsoil As contents (11-35 mg kg(-1), 0-10 cm). Soil As contents after irrigation (May 2005) showed that most As input occurred close to the water inlet and that most As was retained in the top few centimeters of soil. After monsoon flooding (December 2005), topsoil As contents were again close to levels measured before irrigation. Thus, As input during irrigation was at least partly counteracted by As mobilization during monsoon flooding. However, the persisting lateral As distribution suggests net arsenic accumulation over the past 15 years. More pronounced As accumulation may occur in regions with several rice crops per year, less intense monsoon flooding, or different irrigation schemes. The high lateral and vertical heterogeneity of soil As contents must be taken into account in future studies related to As accumulation in paddy soils and potential As transfer into rice.

Reconciling the conservation of the purple swamphen (Porphyrio porphyrio) and its damage in Mediterranean rice fields through sustainable non-lethal techniques

PubMed Central

Piqué, Josep

2018-01-01

Resolving human–wildlife conflicts requires the assessment and implementation of appropriate technical measures that minimize negative impacts on socio-economic uses, including agriculture, and ensure the adequate protection of biological diversity. Rice paddies are widely distributed in the western Mediterranean region. Because of their high productivity, they can be a good habitat for waterbirds, including the purple swamphen Porphyrio porphyrio, particularly in areas where natural wetlands have been removed or reduced. As a result of its population growth, there have been increasing levels of damage caused by this species in rice fields due to stem-cutting and opening of bald patches in rice fields. With the aim of reducing damage, we evaluated the effectiveness of passive and active measures that would limit access to rice fields and deter/scare away purple swamphens in affected areas of the Ebro Delta (NE Spain). We selected the techniques according to the growth phase of rice and the activity of birds in the rice fields (perimeter fences and clearing vegetation around the rice plots during sprouting and growing phases, and falconry at maturation). There were positive results during the sprouting and growing phases thanks to fences and clearing vegetation, reducing the affected area by 37.8% between treatment and control plots. This would mean an economic savings of 18,550 €/year in compensation payments by regional administrations including the investment in implementing and maintaining passive protection measures. Active deterrence through falconry did not reduce the level of damage. The analysis of purple swamphen home range, activity centers (centroids), and the proportion of locations in and outside of rice fields showed no differences before and after dissuasive practices. These results were influenced by multiple concurrent factors including weather, the structural configuration of the rice plots and their location. In summary, we recommend the

Sulfate-reducing bacteria in rice field soil and on rice roots.

PubMed

Wind, T; Stubner, S; Conrad, R

1999-05-01

Rice plants that were grown in flooded rice soil microcosms were examined for their ability to exhibit sulfate reducing activity. Washed excised rice roots showed sulfate reduction potential when incubated in anaerobic medium indicating the presence of sulfate-reducing bacteria. Rice plants, that were incubated in a double-chamber (phylloshpere and rhizosphere separated), showed potential sulfate reduction rates in the anoxic rhizosphere compartment. These rates decreased when oxygen was allowed to penetrate through the aerenchyma system of the plants into the anoxic root compartment, indicating that sulfate reducers on the roots were partially inhibited by oxygen or that sulfate was regenerated by oxidation of reduced S-compounds. The potential activity of sulfate reducers on rice roots was consistent with MPN enumerations showing that H2-utilizing sulfate-reducing bacteria were present in high numbers on the rhizoplane (4.1 x 10(7) g-1 root fresh weight) and in the adjacent rhizosperic soil (2.5 x 10(7) g-1 soil dry weight). Acetate-oxidizing sulfate reducers, on the other hand, showed highest numbers in the unplanted bulk soil (1.9 x 10(6) g-1 soil dry weight). Two sulfate reducing bacteria were isolated from the highest dilutions of the MPN series and were characterized physiologically and phylogenetically. Strain F1-7b which was isolated from the rhizoplane with H2 as electron donor was related to subgroup II of the family Desulfovibrionaceae. Strain EZ-2C2, isolated from the rhizoplane on acetate, grouped together with Desulforhabdus sp. and Syntrophobacter wolinii. Other strains of sulfate-reducing bacteria originated from bulk soil of rice soil microcosms and were isolated using different electron donors. From these isolates, strains R-AcA1, R-IbutA1, R-PimA1 and R-AcetonA170 were Gram-positive bacteria which were affiliated with the genus Desulfotomaculum. The other isolates were members of subgroup II of the Desulfovibrionaceae (R-SucA1 and R-LacA1), were

Epiphytic Cyanobacteria on Chara vulgaris Are the Main Contributors to N2 Fixation in Rice Fields

PubMed Central

Ariosa, Yoanna; Quesada, Antonio; Aburto, Juan; Carrasco, David; Carreres, Ramón; Leganés, Francisco; Fernández Valiente, Eduardo

2004-01-01

The distribution of nitrogenase activity in the rice-soil system and the possible contribution of epiphytic cyanobacteria on rice plants and other macrophytes to this activity were studied in two locations in the rice fields of Valencia, Spain, in two consecutive crop seasons. The largest proportion of photodependent N2 fixation was associated with the macrophyte Chara vulgaris in both years and at both locations. The nitrogen fixation rate associated with Chara always represented more than 45% of the global nitrogenase activity measured in the rice field. The estimated average N2 fixation rate associated with Chara was 27.53 kg of N ha−1 crop−1. The mean estimated N2 fixation rates for the other parts of the system for all sampling periods were as follows: soil, 4.07 kg of N ha−1 crop−1; submerged parts of rice plants, 3.93 kg of N ha−1 crop−1; and roots, 0.28 kg of N ha−1 crop−1. Micrographic studies revealed the presence of epiphytic cyanobacteria on the surface of Chara. Three-dimensional reconstructions by confocal scanning laser microscopy revealed no cyanobacterial cells inside the Chara structures. Quantification of epiphytic cyanobacteria by image analysis revealed that cyanobacteria were more abundant in nodes than in internodes (on average, cyanobacteria covered 8.4% ± 4.4% and 6.2% ± 5.0% of the surface area in the nodes and internodes, respectively). Epiphytic cyanobacteria were also quantified by using a fluorometer. This made it possible to discriminate which algal groups were the source of chlorophyll a. Chlorophyll a measurements confirmed that cyanobacteria were more abundant in nodes than in internodes (on average, the chlorophyll a concentrations were 17.2 ± 28.0 and 4.0 ± 3.8 μg mg [dry weight] of Chara−1 in the nodes and internodes, respectively). These results indicate that this macrophyte, which is usually considered a weed in the context of rice cultivation, may help maintain soil N fertility in the rice field

Biofortified indica rice attains iron and zinc nutrition dietary targets in the field

PubMed Central

Trijatmiko, Kurniawan R.; Dueñas, Conrado; Tsakirpaloglou, Nikolaos; Torrizo, Lina; Arines, Felichi Mae; Adeva, Cheryl; Balindong, Jeanette; Oliva, Norman; Sapasap, Maria V.; Borrero, Jaime; Rey, Jessica; Francisco, Perigio; Nelson, Andy; Nakanishi, Hiromi; Lombi, Enzo; Tako, Elad; Glahn, Raymond P.; Stangoulis, James; Chadha-Mohanty, Prabhjit; Johnson, Alexander A. T.; Tohme, Joe; Barry, Gerard; Slamet-Loedin, Inez H.

2016-01-01

More than two billion people are micronutrient deficient. Polished grains of popular rice varieties have concentration of approximately 2 μg g−1 iron (Fe) and 16 μg g−1 zinc (Zn). The HarvestPlus breeding programs for biofortified rice target 13 μg g−1 Fe and 28 μg g−1 Zn to reach approximately 30% of the estimated average requirement (EAR). Reports on engineering Fe content in rice have shown an increase up to 18 μg g−1 in glasshouse settings; in contrast, under field conditions, 4 μg g−1 was the highest reported concentration. Here, we report on selected transgenic events, field evaluated in two countries, showing 15 μg g−1 Fe and 45.7 μg g−1 Zn in polished grain. Rigorous selection was applied to 1,689 IR64 transgenic events for insert cleanliness and, trait and agronomic performances. Event NASFer-274 containing rice nicotianamine synthase (OsNAS2) and soybean ferritin (SferH-1) genes showed a single locus insertion without a yield penalty or altered grain quality. Endosperm Fe and Zn enrichment was visualized by X-ray fluorescence imaging. The Caco-2 cell assay indicated that Fe is bioavailable. No harmful heavy metals were detected in the grain. The trait remained stable in different genotype backgrounds. PMID:26806528

Field-based rice classification in Wuhua county through integration of multi-temporal Sentinel-1A and Landsat-8 OLI data

NASA Astrophysics Data System (ADS)

Yang, Huijin; Pan, Bin; Wu, Wenfu; Tai, Jianhao

2018-07-01

Rice is one of the most important cereals in the world. With the change of agricultural land, it is urgently necessary to update information about rice planting areas. This study aims to map rice planting areas with a field-based approach through the integration of multi-temporal Sentinel-1A and Landsat-8 OLI data in Wuhua County of South China where has many basins and mountains. This paper, using multi-temporal SAR and optical images, proposes a methodology for the identification of rice-planting areas. This methodology mainly consists of SSM applied to time series SAR images for the calculation of a similarity measure, image segmentation process applied to the pan-sharpened optical image for the searching of homogenous objects, and the integration of SAR and optical data for the elimination of some speckles. The study compares the per-pixel approach with the per-field approach and the results show that the highest accuracy (91.38%) based on the field-based approach is 1.18% slightly higher than that based on the pixel-based approach for VH polarization, which is brought by eliminating speckle noise through comparing the rice maps of these two approaches. Therefore, the integration of Sentinel-1A and Landsat-8 OLI images with a field-based approach has great potential for mapping rice or other crops' areas.

Effects of processing and cooking on the reduction of dinotefuran concentration in Japanese rice samples.

PubMed

Watanabe, Minae; Ueyama, Jun; Ueno, Eiji; Ueda, Yuko; Oda, Masaya; Umemura, Yuko; Tanahashi, Takashi; Ikai, Yoshitomo; Saito, Isao

2018-05-23

Dinotefuran is an insecticide belonging to the neonicotinoid class, which is frequently used to control pests in paddy rice owing to its permeability and effectiveness against sucking insects. Since 2002, this insecticide has been commercially available in Japan, and has become controversial due to its high detection frequency in brown rice for primary consumption. In this study, the effects of processing and cooking on the reduction of dinotefuran residues in commercially available brown rice were investigated. Boiled rice is difficult to homogenise and extract with acetonitrile. Using pre-freezing and cryogenic milling with powdered dry ice, dinotefuran in boiled rice was extracted well. A measurement method comprising sample preparation (acetonitrile extraction, gel permeation chromatography, and SPE) and detection with anLC-MS/MS system was used. In 10 out of 25 commercial brown rice samples, dinotefuran was detected at a concentration of 0.04 μg/g (mean), which was more than the limit of quantitation of 0.01 μg/g. The dinotefuran levels were significantly less than the MRL of 2 μg/g in Japan. Even after polishing, washing, and boiling, dinotefuran was detected in 10 brown rice samples, with mean residue levels of 74.7%, 60.8%, and 39.6%, respectively, of the original concentration in brown rice. Based on these data, the processing factor of dinotefuran in boiled rice has been estimated to be approximately 0.4. Dinotefuran residues were reduced in the boiled rice, but less so than other pesticides. Although the maximum daily intake of dinotefuran in boiled rice was 0.0065 mg/person/day, its percent ratio to the ADI of dinotefuran in Japan was less than 0.05%. These results suggest that the daily intake of dinotefuran from rice might not be a critical problem at present, in spite of its relatively high detection frequency in boiled rice.

Response of rice cultivars to rates of nitrogen and potassium application in field and pot conditions.

PubMed

Bahmaniar, M A; Ranjbar, G A

2007-05-01

Nitrogen and potassium are the yield-limiting nutrients in rice production regions of Iran. Use of N and K efficient cultivars is an important complementary strategy in improving rice yield, increasing the quality properties of rice grains and reducing cost of production. In order to consider the effects of different amounts of N and K application on rice (Oryza sativa L.) yield and yield components in pot and field conditions these experiments were undertaken in 2004 at Sari Agricultural Station, Iran. Four levels of N (0, 50, 100 and 150 Kg N ha(-1) in field and 0, 0.6, 1.2 and 1.8 g N pot(-1) in pot) corresponding with four levels of K (0, 75, 150 and 225 kg K2O ha(-1) in field and 0, 0.5, 1 and 1.5 g K2O pot(-1) in pot) were applied in a split-factorial plot design with three replications in both pot and field experiments, variously. Grain yield, number of grain per panicle, number of tiller, plant height, length of flag leaf, total and shoot dry matter, 1000 grain weight and harvest index have been increased by N application in field conditions. However, in pot conditions grain yield, number of grain per panicle, number of tiller, plant height, width of flag leaf, total and shoot dry matter, leaf nitrogen contents and harvest index have significantly been increased (p < or = 0.05). Potassium application in field conditions has significantly affected on all characteristics but 1000 grain weight and leaf N and K contents. Simultaneous application of N and K have increasingly affected on grain yield, plant height, shoot dry matter and harvest index in field conditions and on plant height, length of flag leaf and shoot dry matter in pot conditions (p < or = 0.05).

Characterization of isolates of meloidogyne from rice-wheat production fields in Nepal.

PubMed

Pokharel, Ramesh R; Abawi, George S; Zhang, Ning; Duxbury, John M; Smart, Christine D

2007-09-01

Thirty-three isolates of root-knot nematode were recovered from soil samples from rice-wheat fields in Nepal and maintained on rice cv. BR 11. The isolates were characterized using morphology, host range and DNA sequence analyses in order to ascertain their identity. Results indicated phenotypic similarity (juvenile measurements, perennial pattern, host range and gall shape) of the Nepalese isolates with Meloidogyne graminicola, with minor variations. The rice varieties LA 110 and Labelle were susceptible to all of the Nepalese isolates, but differences in the aggressiveness of the isolates were observed. Phylogenetic analyses based on the sequences of partial internal transcribed spacer (ITS) of the rRNA genes indicated that all Nepalese isolates formed a distinct clade with known isolates of M. graminicola with high bootstrap support. Furthermore, two groups were identified within the M. graminicola clade. No correlation between ITS haplotype and aggressiveness or host range was found among the tested isolates.

Effects of screenhouse cultivation and organic materials incorporation on global warming potential in rice fields.

PubMed

Xu, Guochun; Liu, Xin; Wang, Qiangsheng; Xiong, Ruiheng; Hang, Yuhao

2017-03-01

Global rice production will be increasingly challenged by providing healthy food for a growing population at minimal environmental cost. In this study, a 2-year field experiment was conducted to investigate the effects of a novel rice cultivation mode (screenhouse cultivation, SHC) and organic material (OM) incorporation (wheat straw and wheat straw-based biogas residue) on methane (CH 4 ) and nitrous oxide (N 2 O) emissions and rice yields. In addition, the environmental factors and soil properties were also determined. Relative to the traditional open-field cultivation (OFC), SHC decreased the CH 4 and N 2 O emissions by 6.58-18.73 and 2.51-21.35%, respectively, and the global warming potential (GWP) was reduced by 6.49-18.65%. This trend was mainly because of lower soil temperature and higher soil redox potential in SHC. Although the rice grain yield for SHC were reduced by 2.51-4.98% compared to the OFC, the CH 4 emissions and GWP per unit of grain yield (yield-scaled CH 4 emissions and GWP) under SHC were declined. Compared to use of inorganic fertilizer only (IN), combining inorganic fertilizer with wheat straw (WS) or wheat straw-based biogas residue (BR) improved rice grain yield by 2.12-4.10 and 4.68-5.89%, respectively. However, OM incorporation enhanced CH 4 emissions and GWP, leading to higher yield-scaled CH 4 emissions and GWP in WS treatment. Due to rice yield that is relatively high, there was no obvious effect of BR treatment on them. These findings suggest that apparent environmental benefit can be realized by applying SHC and fermenting straw aerobically before its incorporation.

Dynamics of the Methanogenic Archaeal Community during Plant Residue Decomposition in an Anoxic Rice Field Soil ▿

PubMed Central

Peng, Jingjing; Lü, Zhe; Rui, Junpeng; Lu, Yahai

2008-01-01

Incorporation of plant residues strongly enhances the methane production and emission from flooded rice fields. Temperature and residue type are important factors that regulate residue decomposition and CH4 production. However, the response of the methanogenic archaeal community to these factors in rice field soil is not well understood. In the present experiment, the structure of the archaeal community was determined during the decomposition of rice root and straw residues in anoxic rice field soil incubated at three temperatures (15°C, 30°C, and 45°C). More CH4 was produced in the straw treatment than root treatment. Increasing the temperature from 15°C to 45°C enhanced CH4 production. Terminal restriction fragment length polymorphism analyses in combination with cloning and sequencing of 16S rRNA genes showed that Methanosarcinaceae developed early in the incubations, whereas Methanosaetaceae became more abundant in the later stages. Methanosarcinaceae and Methanosaetaceae seemed to be better adapted at 15°C and 30°C, respectively, while the thermophilic Methanobacteriales and rice cluster I methanogens were significantly enhanced at 45°C. Straw residues promoted the growth of Methanosarcinaceae, whereas the root residues favored Methanosaetaceae. In conclusion, our study revealed a highly dynamic structure of the methanogenic archaeal community during plant residue decomposition. The in situ concentration of acetate (and possibly of H2) seems to be the key factor that regulates the shift of methanogenic community. PMID:18344350

Biochar amendment reduces rice Cd uptake in polluted and unpolluted paddy soils: a long term field experiment

NASA Astrophysics Data System (ADS)

Bian, R.; Cui, L.; Pan, G.; Li, L.

2012-04-01

The bioavailability of Cd in agricultural soils has been a great health concern due to the potential risk through exposure of agro-food produced in Cd-contaminated fields. Yet, rice subject to Cd contamination appears to have expanded at the last decade due to irrigation with waste water and chemical fertilization in south china. This is supposed to raise the Cd accumulation of rice grain. Therefore, techniques to reduce Cd mobility and plant uptake have been a urgent demand for food safety in China.A field experiment was performed in a high-polluted (HP), mid-pollute (MP) and unpolluted (UP) paddy soil with biochar(BC) amendment in 2011. BC was applied in HP, MP and UP in 2008, 2009, 2009 with the rates of 0, 10, 20, 40t ha-1 in HP, MP and 0, 40t ha-1 in UP. The experiment was monitored in 2011. It was observed that BC amendment did not affect rice grain yield but significantly increased soil pH by 0.58-0.77, 1.30 units in MP, UP and there was no difference in HP. The Cacl2 extracted Cd in soil was decreased by 18.1%-28.9% in HP, 49.3%-67.5% in MP and 83.1% in UP, respectively. Meanwhile, H2O extractable Cd in soil was decreased by 20.0%-31.7% in HP, 32.7%-44.2% in MP and 25.0% in UP, respectively. With the BC treatment, rice grain Cd concentration was decreased 4.7%-17.6% in HP, 35.9%-53.4% in MP. Especially in UP field, the rice grain Cd concentration was decreased from 0.22mg kg-1 to 0.07mg kg-1 which was below National standard (0.20mg kg-1) in China. The straw and root Cd contents were also significantly decreased with BC application. Therefore, BC amendment in polluted and unpolluted fields can sustainably reduce rice Cd uptake and it may offer a basic option to reduce Cd levels in rice. Keywords: Biochar, Cd, bioavailability, paddy soil, food safety

The effect of water regime and soil management on methane (CH4) emission of rice field

NASA Astrophysics Data System (ADS)

Naharia, O.; Setyanto, P.; Arsyad, M.; Burhan, H.; Aswad, M.

2018-05-01

Mitigation of CH4 emission of rice field is becoming a serious issue. The Agricultural Environment Preservation Research Station in Central Java conducted a field study to investigate the effect of water regime and soil tillage on CH4 emission from paddy fields. Treatments consisted of two factors. The first factor was water regime, e.g., 1) continuously flooded 5 cm, 2) intermittent irrigation and 3) saturated water condition at 0-1 cm water level. The second factor was soil management, e.g., 1) normal tillage, 2) zero tillage + 3 sulfosate ha-1 and 3) zero tillage + 3 L paraquat ha-1. Most of treatments gave a significant reduction of total CH4 emission between 34 – 85% during the wet season crop as compared to normal rice cropping practice, while in the dry season the CH4 reduction ranged between 16 – 92%. No-tillage with non-selective herbicides combined with intermittent/saturated irrigation system significantly reduced methane emission without significantly affecting rice productivity as compared to normal tillage with continuous flooding (farmers practice)

Field evaluation of four spatial repellent devices against Arkansas rice-land mosquitoes

USDA-ARS?s Scientific Manuscript database

Four commercially available spatial repellent devices were tested in a rice land habitat near Stuttgart, Arkansas after semi-field level assessments had been made at the Center for Medical, Agricultural, and Veterinary Entomology, ARS, USDA in Gainesville, FL. OFF! Clip-On® (a.i. metofluthrin, S.C....

Exploring a suitable nitrogen fertilizer rate to reduce greenhouse gas emissions and ensure rice yields in paddy fields.

PubMed

Zhong, Yiming; Wang, Xiaopeng; Yang, Jingping; Zhao, Xing; Ye, Xinyi

2016-09-15

The application rate of nitrogen fertilizer was believed to dramatically influence greenhouse gas (GHG) emissions from paddy fields. Thus, providing a suitable nitrogen fertilization rate to ensure rice yields, reducing GHG emissions and exploring emission behavior are important issues for field management. In this paper, a two year experiment with six rates (0, 75, 150, 225, 300, 375kgN/ha) of nitrogen fertilizer application was designed to examine GHG emissions by measuring carbon dioxide (CO2), methane (CH4), nitrous oxide (N2O) flux and their cumulative global warming potential (GWP) from paddy fields in Hangzhou, Zhejiang in 2013 and 2014. The results indicated that the GWP and rice yields increased with an increasing application rate of nitrogen fertilizer. Emission peaks of CH4 mainly appeared at the vegetative phase, and emission peaks of CO2, and N2O mainly appeared at reproductive phase of rice growth. The CO2 flux was significantly correlated with soil temperature, while the CH4 flux was influenced by logging water remaining period and N2O flux was significantly associated with nitrogen application rates. This study showed that 225kgN/ha was a suitable nitrogen fertilizer rate to minimize GHG emissions with low yield-scaled emissions of 3.69 (in 2013) and 2.23 (in 2014) kg CO2-eq/kg rice yield as well as to ensure rice yields remained at a relatively high level of 8.89t/ha in paddy fields. Copyright © 2016 Elsevier B.V. All rights reserved.

A digital photography and analysis system for estimation of root and shoot development in rice weed suppression studies in the field

USDA-ARS?s Scientific Manuscript database

Rice germplasm with an inherent ability to suppress weeds can potentially improve the economics and sustainability of weed control in rice. We devised a simple, rapid, and inexpensive digital imaging system to quantify several shoot and root growth characteristics in field-grown rice plants that ha...

Mitigation of cadmium and arsenic in rice grain by applying different silicon fertilizers in contaminated fields.

PubMed

Wang, Hong-Yan; Wen, Shi-Lin; Chen, Peng; Zhang, Lu; Cen, Kuang; Sun, Guo-Xin

2016-02-01

A field experiment was established to support the hypothesis that application of different silicon (Si) fertilizers can simultaneously reduce cadmium (Cd) and arsenic (As) concentration in rice grain. The "semi-finished product of Si-potash fertilizer" treatment at the high application of 9000 kg/ha (NP+S-KSi9000) significantly reduced the As concentration in rice grain by up to 20.1%, compared with the control. Si fertilization reduces the Cd concentration in rice considerably more than the As concentration. All Si fertilizers apart from sodium metasilicate (Na2SiO3) exhibited a high ability to reduce Cd concentration in rice grain. The Si-calcium (CaSi) fertilizer is the most effective in the mitigation of Cd concentration in rice grain. The CaSi fertilizer applied at 9000 kg/ha (NPK+CaSi9000) and 900 kg/ha (NPK+CaSi900) reduced the Cd concentration in rice grain about 71.5 and 48.0%, respectively, while the Si-potash fertilizer at 900 kg/ha (NP+KSi900), the semi-finished product of Si-potash fertilizer at both 900 kg/ha (NP+S-KSi900) and 9000 kg/ha (NP+S-KSi9000), and the rice straw (NPK+RS) treatments reduced the Cd concentration in rice grain about 42, 26.5, 40.7, and 23.1%, respectively. The results of this investigation demonstrated the potential effects of Si fertilizers in reducing Cd and As concentrations in rice grain.

Effects of Residue Management on Decomposition in Irrigated Rice Fields Are Not Related to Changes in the Decomposer Community.

PubMed

Schmidt, Anja; John, Katharina; Arida, Gertrudo; Auge, Harald; Brandl, Roland; Horgan, Finbarr G; Hotes, Stefan; Marquez, Leonardo; Radermacher, Nico; Settele, Josef; Wolters, Volkmar; Schädler, Martin

2015-01-01

Decomposers provide an essential ecosystem service that contributes to sustainable production in rice ecosystems by driving the release of nutrients from organic crop residues. During a single rice crop cycle we examined the effects of four different crop residue management practices (rice straw or ash of burned straw scattered on the soil surface or incorporated into the soil) on rice straw decomposition and on the abundance of aquatic and soil-dwelling invertebrates. Mass loss of rice straw in litterbags of two different mesh sizes that either prevented or allowed access of meso- and macro-invertebrates was used as a proxy for decomposition rates. Invertebrates significantly increased total loss of litter mass by up to 30%. Initially, the contribution of invertebrates to decomposition was significantly smaller in plots with rice straw scattered on the soil surface; however, this effect disappeared later in the season. We found no significant responses in microbial decomposition rates to management practices. The abundance of aquatic fauna was higher in fields with rice straw amendment, whereas the abundance of soil fauna fluctuated considerably. There was a clear separation between the overall invertebrate community structure in response to the ash and straw treatments. However, we found no correlation between litter mass loss and abundances of various lineages of invertebrates. Our results indicate that invertebrates can contribute to soil fertility in irrigated paddy fields by decomposing rice straw, and that their abundance as well as efficiency in decomposition may be promoted by crop residue management practices.

[Tasseled cap triangle (TCT)-leaf area index (LAI)model of rice fields based on PROSAIL model and its application].

PubMed

Li, Ya Ni; Lu, Lei; Liu, Yong

2017-12-01

The tasseled cap triangle (TCT)-leaf area index (LAI) isoline is a model that reflects the distribution of LAI isoline in the spectral space constituted by reflectance of red and near-infrared (NIR) bands, and the LAI retrieval model developed on the basis of this is more accurate than the commonly used statistical relationship models. This study used ground-based measurements of the rice field, validated the applicability of PROSAIL model in simulating canopy reflectance of rice field, and calibrated the input parameters of the model. The ranges of values of PROSAIL input parameters for simulating rice canopy reflectance were determined. Based on this, the TCT-LAI isoline model of rice field was established, and a look-up table (LUT) required in remote sensing retrieval of LAI was developed. Then, the LUT was used for Landsat 8 and WorldView 3 data to retrieve LAI of rice field, respectively. The results showed that the LAI retrieved using the LUT developed from TCT-LAI isoline model had a good linear relationship with the measured LAI R 2 =0.76, RMSE=0.47. Compared with the LAI retrieved from Landsat 8, LAI values retrieved from WorldView 3 va-ried with wider range, and data distribution was more scattered. Resampling the Landsat 8 and WorldView 3 reflectance data to 1 km to retrieve LAI, the result of MODIS LAI product was significantly underestimated compared to that of retrieved LAI.

Application of a rotation system to oilseed rape and rice fields in Cd-contaminated agricultural land to ensure food safety.

PubMed

Yu, Lingling; Zhu, Junyan; Huang, Qingqing; Su, Dechun; Jiang, Rongfeng; Li, Huafen

2014-10-01

This field experiment analyzed the phytoremediation effects of oilseed rape in moderately cadmium (Cd)-contaminated farmland and the food safety of successive rice in an oilseed rape-rice rotation system. Two oilseed rape cultivars accumulated Cd at different rates. The rapeseed cultivar Zhucang Huazi exhibited high Cd accumulation rates, higher than the legal limit for human consumption (0.2mgkg(-1)); Cd concentrations in the cultivar Chuanyou II-93 were all below the maximum allowed level. Planting oilseed rape increased the uptake of Cd by the successive rice crop compared with a previous fallow treatment. Most Cd concentrations of brown rice were below the maximum allowed level. The phytoextraction efficiency was lower in the moderately Cd-contaminated soil in field experiments. The results suggest screening rice cultivars with lower Cd accumulation can assure the food safety; the mobilization of heavy metals by roots of different plant species should be considered during crop rotation to assure food safety. Copyright © 2014 Elsevier Inc. All rights reserved.

Influence of transgenic rice expressing a fused Cry1Ab/1Ac protein on frogs in paddy fields.

PubMed

Wang, Jia-Mei; Chen, Xiu-Ping; Liang, Yu-Yong; Zhu, Hao-Jun; Ding, Jia-Tong; Peng, Yu-Fa

2014-11-01

As genetic engineering in plants is increasingly used to control agricultural pests, it is important to determine whether such transgenic plants adversely affect non-target organisms within and around cultivated fields. The cry1Ab/1Ac fusion gene from Bacillus thuringiensis (Bt) has insecticidal activity and has been introduced into rice line Minghui 63 (MH63). We evaluated the effect of transgenic cry1Ab/1Ac rice (Huahui 1, HH1) on paddy frogs by comparing HH1 and MH63 rice paddies with and without pesticide treatment. The density of tadpoles in rice fields was surveyed at regular intervals, and Cry1Ab/1Ac protein levels were determined in tissues of tadpoles and froglets collected from the paddy fields. In addition, Rana nigromaculata froglets were raised in purse nets placed within these experimental plots. The survival, body weight, feeding habits, and histological characteristics of the digestive tract of these froglets were analyzed. We found that the tadpole density was significantly decreased immediately after pesticide application, and the weight of R. nigromaculata froglets of pesticide groups was significantly reduced compared with no pesticide treatment, but we found no differences between Bt and non-Bt rice groups. Moreover, no Cry1Ab/1Ac protein was detected in tissue samples collected from 192 tadpoles and froglets representing all four experimental groups. In addition, R. nigromaculata froglets raised in purse seines fed primarily on stem borer and non-target insects, and showed no obvious abnormality in the microstructure of their digestive tracts. Based on these results, we conclude that cultivation of transgenic cry1Ab/1Ac rice does not adversely affect paddy frogs.

Seasonal trends and environmental controls of methane emissions in a rice paddy field in Northern Italy

NASA Astrophysics Data System (ADS)

Meijide, A.; Manca, G.; Goded, I.; Magliulo, V.; di Tommasi, P.; Seufert, G.; Cescatti, A.

2011-12-01

Rice paddy fields are one of the greatest anthropogenic sources of methane (CH4), the third most important greenhouse gas after water vapour and carbon dioxide. In agricultural fields, CH4 is usually measured with the closed chamber technique, resulting in discontinuous series of measurements performed over a limited area, that generally do not provide sufficient information on the short-term variation of the fluxes. On the contrary, aerodynamic techniques have been rarely applied for the measurement of CH4 fluxes in rice paddy fields. The eddy covariance (EC) technique provides integrated continuous measurements over a large area and may increase our understanding of the underlying processes and diurnal and seasonal pattern of CH4 emissions in this ecosystem. For this purpose a Fast Methane Analyzer (Los Gatos Research Ltd.) was installed in a rice paddy field in the Po Valley (Northern Italy). Methane fluxes were measured during the rice growing season with both EC and manually operated closed chambers. Methane fluxes were strongly influenced by the height of the water table, with emissions peaking when it was above 10-12 cm. Soil temperature and the developmental stage of rice plants were also responsible of the seasonal variation on the fluxes. The measured EC fluxes showed a diurnal cycle in the emissions, which was more relevant during the vegetative period, and with CH4 emissions being higher in the late evening, possibly associated with higher water temperature. The comparison between the two measurement techniques shows that greater fluxes are measured with the chambers, especially when higher fluxes are being produced, resulting in 30 % higher seasonal estimations with the chambers than with the EC (41.1 and 31.7 g CH4 m-2 measured with chambers and EC respectively) and even greater differences are found if shorter periods with high chamber sampling frequency are compared. The differences may be a result of the combined effect of overestimation with the

Risk factors for coronary artery calcification in Japanese patients.

PubMed

Shikada, Tomoki; Washio, Masakazu; Nishizaki, Akiko; Kakino, Takamori; Ooe, Kensuke; Ishibashi, Yuuji; Sagara, Shuuichirou; Morishige, Kunio; Tashiro, Hideki

2015-07-01

Because the prevalence of coronary artery calcification is lower among Japanese than among Western individuals, aspects of the Japanese lifestyle might be related to the development of calcification. We aimed to clarify the relationship between coronary artery calcium scores in Japanese patients and various lifestyle factors among the Japanese population. Study subjects were patients aged ≥20 years who underwent multidetector-row computed tomography. A total of 201 patients agreed to take part in this study and answered a questionnaire regarding lifestyle, medical history, and other factors. Old age, current and former smoking, sedentary work, short sleep time, coronary artery stenosis, treatment with statins, medical history of cerebrovascular disease, medical history of angina pectoris, medical history of ischemic heart disease, and medical history of dyslipidemia were associated with higher odds ratios than the other factors examined, while the Japanese-style breakfast (e.g. boiled rice, miso soup, grilled fish) was associated with lower odds ratios. In this study, smoking, sedentary work, short sleep time, and the Japanese-style breakfast were lifestyle factors related to coronary artery calcification. The lifestyle of Japanese people may be related to coronary calcification. Copyright © 2014 Japanese College of Cardiology. Published by Elsevier Ltd. All rights reserved.

Genetic diversity of red-grained rice landraces in Hani's terraced fields based on phenotypic characteristics

NASA Astrophysics Data System (ADS)

Zhou, Xiaomei; Zheng, Yun; Zhang, Tingting; Zhang, Xiaoqian; Ma, Mengli; Meng, Hengling; Wang, Tiantao; Lu, Bingyue

2018-06-01

In order to provide useful information for protection and utilization of red-grained rice landraces from Hani's terraced fields, the phenotypic diversity of 61 red-grained rice landraces were assessed based 20 quantitative traits. The results indicated that the phenotypic diversity was abundant in red-grained rice landraces. Coefficients of variation (CV) ranged from 4.878% to 72.878%, and the largest of CV was the panicle neck length, while grain width was smallest. Shannon-Weaver diversity index (H') of 20 traits ranged from 1.464 to 2.165, the largest and the smallest H' values were observed in filled grain number and chalkiness, respectively. Cluster analysis based on unweighted pair group method showed 61 red-grain rice landraces grouped into eight clusters at a cut-off value of 6.2631. The first cluster included 11 landraces, the main cluster II involved 42 landraces, and the cluster IV included 3 landraces. Laopinzhonghongmi, Chena2, Laojingnuo, Bianhao6 and Baimi were separated from the main clusters.

Seasonal trends and environmental controls of methane emissions in a rice paddy field in Northern Italy

NASA Astrophysics Data System (ADS)

Meijide, A.; Manca, G.; Goded, I.; Magliulo, V.; di Tommasi, P.; Seufert, G.; Cescatti, A.

2011-09-01

Rice paddy fields are one of the greatest anthropogenic sources of methane (CH4), the third most important greenhouse gas after water vapour and carbon dioxide. In agricultural fields, CH4 is usually measured with the closed chamber technique, resulting in discontinuous series of measurements performed over a limited area, that generally do not provide sufficient information on the short-term variation of the fluxes. On the contrary, aerodynamic techniques have been rarely applied for the measurement of CH4 fluxes in rice paddy fields. The eddy covariance (EC) technique provides integrated continuous measurements over a large area and may increase our understanding of the underlying processes and diurnal and seasonal pattern of CH4 emissions in this ecosystem. For this purpose a Fast Methane Analyzer (Los Gatos Research Ltd.) was installed in an eddy-covariance field set-up in a rice paddy field in the Po Valley (Northern Italy). Methane fluxes were measured during the rice growing season, both with EC and with manually operated closed chambers. Methane fluxes were strongly influenced by the presence of the water table, with emissions peaking when it was above 10-12 cm. Further studies are required to evaluate if water table management could decrease CH4 emissions. The development of rice plants and soil temperature were also responsible of the seasonal variation on the fluxes. The EC measured showed a diurnal cycle in the emissions, which was more relevant during the vegetative period, and with CH4 emissions being higher in the late evening, possibly associated with higher water temperature. The comparison between both measurement techniques shows that greater fluxes are measured with the chambers, especially when higher fluxes are being produced, resulting in 30 % higher seasonal estimations with the chambers than with the EC (41.1 and 31.8 g CH4 m-2 measured with chambers and EC respectively). The differences may be a result of the combined effect of

The use of Chironomus riparius larvae to assess effects of pesticides from rice fields in adjacent freshwater ecosystems.

PubMed

Faria, Mafalda S; Nogueira, António J A; Soares, Amadeu M V M

2007-06-01

A bioassay with Chironomus riparius larvae, using larval development and growth as endpoints, was carried out inside a rice field and in the adjacent wetland channel in Portugal, during pesticide treatments (molinate, endosulfan and propanil) to determine impact caused by pesticide contamination in freshwater ecosystems. The bioassay was also performed under laboratory conditions, to assess whether in situ and laboratory bioassays demonstrated comparable results. Growth was inhibited by concentrations of endosulfan (2.3 and 1.9 microgL(-1) averages) in water from rice field in both the field and laboratory, and by concentrations of endosulfan (0.55 and 0.76 microgL(-1) averages) in water from the wetland channel in the laboratory bioassay, while development was not affected. C. riparius larvae were not affected by molinate and propanil concentrations. The results indicate that endosulfan treatments in rice fields may cause an ecological impairment in adjacent freshwater ecosystems. The results also indicate that laboratory testing can be used to assess in situ toxicity caused by pesticide contamination.

Can the co-cultivation of rice and fish help sustain rice production?

NASA Astrophysics Data System (ADS)

Hu, Liangliang; Zhang, Jian; Ren, Weizheng; Guo, Liang; Cheng, Yongxu; Li, Jiayao; Li, Kexin; Zhu, Zewen; Zhang, Jiaen; Luo, Shiming; Cheng, Lei; Tang, Jianjun; Chen, Xin

2016-06-01

Because rice feeds half of the world’s population, a secure global food supply depends on sustainable rice production. Here we test whether the co-cultivation of rice and fish into one “rice-fish system” (RFS; fish refers to aquatic animals in this article) could help sustain rice production. We examined intensive and traditional RFSs that have been widely practiced in China. We found that rice yields did not decrease when fish yield was below a threshold value in each intensive RFS. Below the thresholds, moreover, fish yields in intensive RFSs can be substantially higher than those in traditional RFS without reducing rice yield. Relative to rice monoculture, the use of fertilizer-nitrogen and pesticides decreased, and the farmers’ net income increased in RFSs. The results suggest that RFSs can help sustain rice production, and suggest that development of co-culture technologies (i.e. proper field configuration for fish and rice) is necessary to achieve the sustainability.

Effects of Residue Management on Decomposition in Irrigated Rice Fields Are Not Related to Changes in the Decomposer Community

PubMed Central

Schmidt, Anja; John, Katharina; Arida, Gertrudo; Auge, Harald; Brandl, Roland; Horgan, Finbarr G.; Hotes, Stefan; Marquez, Leonardo; Radermacher, Nico; Settele, Josef; Wolters, Volkmar; Schädler, Martin

2015-01-01

Decomposers provide an essential ecosystem service that contributes to sustainable production in rice ecosystems by driving the release of nutrients from organic crop residues. During a single rice crop cycle we examined the effects of four different crop residue management practices (rice straw or ash of burned straw scattered on the soil surface or incorporated into the soil) on rice straw decomposition and on the abundance of aquatic and soil-dwelling invertebrates. Mass loss of rice straw in litterbags of two different mesh sizes that either prevented or allowed access of meso- and macro-invertebrates was used as a proxy for decomposition rates. Invertebrates significantly increased total loss of litter mass by up to 30%. Initially, the contribution of invertebrates to decomposition was significantly smaller in plots with rice straw scattered on the soil surface; however, this effect disappeared later in the season. We found no significant responses in microbial decomposition rates to management practices. The abundance of aquatic fauna was higher in fields with rice straw amendment, whereas the abundance of soil fauna fluctuated considerably. There was a clear separation between the overall invertebrate community structure in response to the ash and straw treatments. However, we found no correlation between litter mass loss and abundances of various lineages of invertebrates. Our results indicate that invertebrates can contribute to soil fertility in irrigated paddy fields by decomposing rice straw, and that their abundance as well as efficiency in decomposition may be promoted by crop residue management practices. PMID:26225556

Influence of harvester and weather conditions on field loss and milling quality of rough rice

USDA-ARS?s Scientific Manuscript database

A better understanding of factors affecting field losses and milling quality during harvest is needed to improve the economic value of rice. The main objective of this study was to evaluate the impact of harvester header, harvester type, and weather conditions on field loss and milling quality of ro...

Multisensor Capacitance Probes for Simultaneously Monitoring Rice Field Soil-Water- Crop-Ambient Conditions.

PubMed

Brinkhoff, James; Hornbuckle, John; Dowling, Thomas

2017-12-26

Multisensor capacitance probes (MCPs) have traditionally been used for soil moisture monitoring and irrigation scheduling. This paper presents a new application of these probes, namely the simultaneous monitoring of ponded water level, soil moisture, and temperature profile, conditions which are particularly important for rice crops in temperate growing regions and for rice grown with prolonged periods of drying. WiFi-based loggers are used to concurrently collect the data from the MCPs and ultrasonic distance sensors (giving an independent reading of water depth). Models are fit to MCP water depth vs volumetric water content (VWC) characteristics from laboratory measurements, variability from probe-to-probe is assessed, and the methodology is verified using measurements from a rice field throughout a growing season. The root-mean-squared error of the water depth calculated from MCP VWC over the rice growing season was 6.6 mm. MCPs are used to simultaneously monitor ponded water depth, soil moisture content when ponded water is drained, and temperatures in root, water, crop and ambient zones. The insulation effect of ponded water against cold-temperature effects is demonstrated with low and high water levels. The developed approach offers advantages in gaining the full soil-plant-atmosphere continuum in a single robust sensor.

Gas film retention and underwater photosynthesis during field submergence of four contrasting rice genotypes

PubMed Central

Winkel, Anders; Pedersen, Ole; Ella, Evangelina; Ismail, Abdelbagi M.; Colmer, Timothy D.

2014-01-01

Floods can completely submerge some rice (Oryza sativa L.) fields. Leaves of rice have gas films that aid O2 and CO2 exchange under water. The present study explored the relationship between gas film persistence and underwater net photosynthesis (PN) as influenced by genotype and submergence duration. Four contrasting genotypes (FR13A, IR42, Swarna, and Swarna-Sub1) were submerged for 13 days in the field and leaf gas films, chlorophyll, and the capacity for underwater PN at near ambient and high CO2 were assessed with time of submergence. At high CO2 during the PN assay, all genotypes initially showed high rates of underwater PN, and this rate was not affected by time of submergence in FR13A. This superior photosynthetic performance of FR13A was not evident in Swarna-Sub1 (carrying the SUB1 QTL) and the declines in underwater PN in both Swarna-Sub1 and Swarna were equal to that in IR42. At near ambient CO2 concentration, underwater PN declined in all four genotypes and this corresponded with loss of leaf gas films with time of submergence. FR13A retained leaf gas films moderately longer than the other genotypes, but gas film retention was not linked to SUB1. Diverse rice germplasm should be screened for gas film persistence during submergence, as this trait could potentially increase carbohydrate status and internal aeration owing to increased underwater PN, which contributes to submergence tolerance in rice. PMID:24759881

Green-house gas emissions from rice fields under different water management

NASA Astrophysics Data System (ADS)

Lagomarsino, Alessandra; Elio Agnelli, Alessandro; Ferrara, Rossana Monica; Adviento-Borbe, Maria Arlene; Linquist, Bruce; Gavina, Giacomo; Ravaglia, Stefano

2013-04-01

During 2012 season, two rice fields have been selected in Italy (Cantaglia farm, Bologna province) and subjected to different water management: one under continuous flooding (WET) and the other under alternate wetting and drying (AWD). In AWD, re-flushing occurred in order to maintain water field capacity over 60 %. Two rice varieties (one commonly cultivated in Italy and one variety from the S.I.S. germoplasm collection) have been considered under WET treatment (Gladio and Zhen Long 13 - abbreviated as ZL13), while only Gladio under AWD. Green house gases (GHGs) sampling have been performed weekly or bi-weekly throughout the growing season. Soluble organic carbon (C), soluble nitrogen (N) and nitrates have been collected through piezometers. Soil sampling have been performed at the beginning and at the end of the growing season and total organic C (TOC), total N (TN), C/N ratio of soil organic matter (SOM), bulk density and water holding capacity were measured. At the end of the growing season rice above- and below-ground biomass have been sampled and C and N content of stem, grain and roots were measured. Methane (CH4) emissions showed a clear trend, following water availability in soils. An initial peak after the first flooding was observed in all soils, while after the second flooding CH4 was emitted only in the WET treatment. Further flooding events in AWD soil did not determine CH4 emissions during the vegetative season. Overall, in 2012 growing season a 98 % reduction of CH4 emissions in AWD soil was observed. In the WET treatment, no significant variations were observed between the two varieties, although on average ZL13 showed lower rates of CH4 emissions. Two peaks of nitrous oxide (N2O) emissions were observed: the first after the initial flooding in all soils; the second one, much greater, 14 days after the fertilization only in AWD soils. These two peaks accounted for 92 % of total N2O emissions in 2012 rice season. Overall, in 2012 growing season N2O

Rice growth monitoring using simulated compact polarimetric C band SAR

NASA Astrophysics Data System (ADS)

Yang, Zhi; Li, Kun; Liu, Long; Shao, Yun; Brisco, Brian; Li, Weiguo

2014-12-01

In this study, a set of nine compact polarimetric (CP) images were simulated from polarimetric RADARSAT-2 data acquired over a test site containing two types of rice field in Jiangsu province, China. The types of rice field in the test site were (1) transplanted hybrid rice fields, and (2) direct-sown japonica rice fields. Both types have different yields and phenological stages. As a first step, the two types of rice field were distinguished with 94% and 86% accuracy respectively through analyzing CP synthetic aperture radar (SAR) observations and their behavior in terms of scattering mechanisms during the rice growth season. The focus was then on phenology retrieval for each type of rice field. A decision tree (DT) algorithm was built to fulfill the precise retrieval of rice phenological stages, in which seven phenological stages were discriminated. The key criterion for each phenological stage was composed of 1-4 CP parameters, some of which were first used for rice phenology retrieval and found to be very sensitive to rice phenological changes. The retrieval results were verified at parcel level for a set of 12 stands of rice and up to nine observation dates per stand. This gave an accuracy of 88-95%. Throughout the phenology retrieval process, only simulated CP data were used, without any auxiliary data. These results demonstrate the potential of CP SAR for rice growth monitoring applications.

[Control effects of rice-duck farming and other weed management strategies on weed communities in paddy fields].

PubMed

Wei, Shouhui; Qiang, Sheng; Ma, Bo; Wei, Jiguang; Chen, Jianwei; Wu, Jianqiang; Xie, Tongzhou; Shen, Xiaokun

2005-06-01

By the methods of community ecology, field studies were conducted to evaluate the control effects of three weed management strategies, i. e., rice-duck farming (RD), manual weeding (MW) and chemical weeding (CW), on the weed communities in paddy fields. The results showed that under rice-duck farming, the weed density in paddy fields decreased significantly, and the control effects on dominant weed species such as Monochoria vaginalis, Cyperus difformis, Sagittaria pygmaea were all above 95%, with an overall effect higher than CW and MW. Under RD, the species richness and Shannon-Wiener diversity indices decreased slightly, while Pielou community evenness indices increased markedly, indicating that the species composition of weed community was greatly improved, and the infestation of former dominant weed species was reduced. The structure of weed communities in paddy fields varied with different weed management strategies, e. g., under RD, Lindernia procumbens, Cyperus difformis and Fimbristylis miliacea constituted the major weed community, and the Whittaker index was significant higher than that of CW, MW and CK, which indicated that rice-duck farming had a greater effect on the structure of the weed communities. The same conclusion could be drawn from Sorensen's similarity indices and cluster analysis with Sorensen's index as the distance measurement.

Effects of Japanese beetle (Coleoptera: Scarabaeidae) and silk clipping in field corn.

PubMed

Steckel, Sandy; Stewart, S D; Tindall, K V

2013-10-01

Japanese beetle (Popillia japonica Newman) is an emerging silk-feeding insect found in fields in the lower Corn Belt and Midsouthern United States. Studies were conducted in 2010 and 2011 to evaluate how silk clipping in corn affects pollination and yield parameters. Manually clipping silks once daily had modest effects on yield parameters. Sustained clipping by either manually clipping silks three times per day or by caging Japanese beetles onto ears affected total kernel weight if it occurred during early silking (R1 growth stage). Manually clipping silks three times per day for the first 5 d of silking affected the number of kernels per ear, total kernel weight, and the weight of individual kernels. Caged beetles fed on silks and, depending on the number of beetles caged per ear, reduced the number of kernels per ear. Caging eight beetles per ear significantly reduced total kernel weight compared with noninfested ears. Drought stress before anthesis appeared to magnify the impact of silk clipping by Japanese beetles. There was evidence of some compensation for reduced pollination by increasing the size of pollinated kernels within the ear. Our results showed that it requires sustained silk clipping during the first week of silking to have substantial impacts on pollination and yield parameters, at least under good growing conditions. Some states recommend treating for Japanese beetle when three Japanese beetles per ear are found, silks are clipped to < 13 mm, and pollination is < 50% complete, and that recommendation appears to be adequate.

Influence of water management and fertilizer application on (137)Cs and (133)Cs uptake in paddy rice fields.

PubMed

Wakabayashi, Shokichi; Itoh, Sumio; Kihou, Nobuharu; Matsunami, Hisaya; Hachinohe, Mayumi; Hamamatsu, Shioka; Takahashi, Shigeru

2016-06-01

Cesium-137 derived from the Tokyo Electric Power Company's Fukushima Dai-ichi Nuclear Power Plant (FDNPP) accident contaminated large areas of agricultural land in Eastern Japan. Previous studies before the accident have indicated that flooding enhances radiocesium uptake in rice fields. We investigated the influence of water management in combination with fertilizers on (137)Cs concentrations in rice plants at two fields in southern Ibaraki Prefecture. Stable Cs ((133)Cs) in the plants was also determined as an analogue for predicting (137)Cs behavior after long-term aging of soil (137)Cs. The experimental periods comprised 3 y starting from 2012 in one field, and 2 y from 2013 in another field. These fields were divided into three water management sections: a long-flooding section without midsummer drainage, and medial-flooding, and short-flooding sections with one- or two-week midsummer drainage and earlier end of flooding than the long-flooding section. Six or four types of fertilizer subsections (most differing only in potassium application) were nested in each water management section. Generally, the long-flooding treatment led to higher (137)Cs and (133)Cs concentrations in both straw and brown rice than medial- and short-flooding treatments, although there were some notable exceptions in the first experimental year at each site. Effects of differing potassium fertilizer treatments were cumulative; the effects on (137)Cs and (133)Cs concentrations in rice plants were not obvious in 2012 and 2013, but in 2014, these concentrations were highest where potassium fertilizer had been absent and lowest where basal dressings of K had been tripled. The relationship between (137)Cs and (133)Cs in rice plants was not correlative in the first experimental year at each site, but correlation became evident in the subsequent year(s). This study demonstrates a novel finding that omitting midsummer drainage and/or delaying drainage during the grain-filling period enhances

[China's rice field greenhouse gas emission under climate change based on DNDC model simulation].

PubMed

Tian, Zhan; Niu, Yi-long; Sun, Lai-xiang; Li, Chang-sheng; Liu, Chun-jiang; Fan, Dong-li

2015-03-01

In contrast to a large body of literature assessing the impact of agriculture greenhouse gas (GHG) emissions on climate change, there is a lack of research examining the impact of climate change on agricultural GHG emissions. This study employed the DNDC v9.5, a state-of-art biogeochemical model, to simulate greenhouse gas emissions in China' s rice-growing fields during 1971-2010. The results showed that owing to temperature rising (on average 0.49 °C higher in the second 20 years than in the first 20 year) and precipitation increase (11 mm more in the second 20 years than in the first 20 years) during the rice growing season, CH4 and N2O emissions in paddy field increased by 0.25 kg C . hm-2 and 0.25 kg N . hm-2, respectively. The rising temperature accelerated CH4 emission and N2O emission increased with precipitation. These results indicated that climate change exerted impact on the mechanism of GHG emissions in paddy field.

Internal aeration of paddy field rice (Oryza sativa) during complete submergence---importance of light and floodwater O2.

PubMed

Winkel, Anders; Colmer, Timothy D; Ismail, Abdelbagi M; Pedersen, Ole

2013-03-01

Flash floods can submerge paddy field rice (Oryza sativa), with adverse effects on internal aeration, sugar status and survival. Here, we investigated the in situ aeration of roots of rice during complete submergence, and elucidated how underwater photosynthesis and floodwater pO(2) influence root aeration in anoxic soil. In the field, root pO(2) was measured using microelectrodes during 2 d of complete submergence. Leaf gas films that formed on the superhydrophobic leaves were left intact, or experimentally removed, to elucidate their effect on internal aeration. In darkness, root pO(2) declined to very low concentrations (0.24 kPa) and was strongly correlated with floodwater pO(2). In light, root pO(2) was high (14 kPa) and primarily a function of the incident light determining the rates of underwater net photosynthesis. Plants with intact leaf gas films maintained higher underwater net photosynthesis relative to plants without gas films when the submerged shoots were in light. During complete submergence, internal aeration of rice in the field relies on underwater photosynthesis during the day and entry of O(2) from the floodwater during the night. Leaf gas films enhance photosynthesis during submergence leading to improved O(2) production and sugar status, and therefore contribute to the submergence tolerance of rice. © 2012 The Authors. New Phytologist © 2012 New Phytologist Trust.

Effect of lambda cyhalothrin on Calothrix sp. (GUEco 1001), an autochthonous cyanobacterium of rice fields of Brahmaputra floodplain.

PubMed

Gupta, Kiran; Baruah, P P

2015-12-01

Pesticide contamination in the rice fields has manifested into a serious global environmental concern. Application of pesticides in the rice fields has deleterious effects on non-target organisms including nitrogen-fixing cyanobacteria which help to maintain the rice field fertility. In the present research endeavor, the effect of lambda cyhalothrin (5% EC), a synthetic pyrethroid insecticide, has been studied on the growth and pigments content of Calothrix sp. (GUEco 1001), an indigenous strain isolated from rice grown areas of Brahmaputra floodplain. To study the toxic effect of lambda cyhalothrin, the test organism was exposed to varying concentrations of the insecticide i.e., 20 ppm, 40 ppm, 80 ppm, and 160 ppm based upon the determination of LC50 for a period of 20 days. The result obtained in the laboratory showed a progressive decrease in the growth and pigments content by the test organism with increasing concentrations of the lambda cyhalothrin against time dose-dependent manner. At high dose (160 ppm), the test organism showed significant decrease in dry weight biomass (54.5%), chlorophyll-a (68%), carotenoids (38%), phycocyanin (80%), and nitrogen contents (55%) over the control. A little but insignificant stimulatory effect on growth and chlorophyll-a contents was recorded in 20 ppm treatment of the insecticide that, however, was reversed in case of carotenoids and phycocyanin contents.

Gas film retention and underwater photosynthesis during field submergence of four contrasting rice genotypes.

PubMed

Winkel, Anders; Pedersen, Ole; Ella, Evangelina; Ismail, Abdelbagi M; Colmer, Timothy D

2014-07-01

Floods can completely submerge some rice (Oryza sativa L.) fields. Leaves of rice have gas films that aid O2 and CO2 exchange under water. The present study explored the relationship between gas film persistence and underwater net photosynthesis (PN) as influenced by genotype and submergence duration. Four contrasting genotypes (FR13A, IR42, Swarna, and Swarna-Sub1) were submerged for 13 days in the field and leaf gas films, chlorophyll, and the capacity for underwater PN at near ambient and high CO2 were assessed with time of submergence. At high CO2 during the PN assay, all genotypes initially showed high rates of underwater PN, and this rate was not affected by time of submergence in FR13A. This superior photosynthetic performance of FR13A was not evident in Swarna-Sub1 (carrying the SUB1 QTL) and the declines in underwater PN in both Swarna-Sub1 and Swarna were equal to that in IR42. At near ambient CO2 concentration, underwater PN declined in all four genotypes and this corresponded with loss of leaf gas films with time of submergence. FR13A retained leaf gas films moderately longer than the other genotypes, but gas film retention was not linked to SUB1. Diverse rice germplasm should be screened for gas film persistence during submergence, as this trait could potentially increase carbohydrate status and internal aeration owing to increased underwater PN, which contributes to submergence tolerance in rice. © The Author 2014. Published by Oxford University Press on behalf of the Society for Experimental Biology.

Effect of allelopathic rice varieties combined with cultural management options on paddy field weeds.

PubMed

Kong, Chui-Hua; Hu, Fei; Wang, Peng; Wu, Jing-Lun

2008-03-01

A number of techniques, including cultural management, allelopathy and bioherbicide, have been considered as alternatives for synthetic herbicides, but successful weed control will require the careful integration of these multiple techniques. This study was conducted to assess the use of allelopathic rice varieties in combination with cultural management options on paddy weeds, in order to develop an allelopathy-based technique to reduce herbicide use in paddies. The weed-suppressive effects of the rice varieties tested varied highly with allelopathic trait, planting pattern and cultural management including planting density, flooding depth and duration and supply of nitrogen. Allelopathic rice varieties PI312777 and Huagan-1 demonstrated much stronger weed suppression than the non-allelopathic variety Huajianxian under the same planting pattern and cultural management. Their weed-suppressive effect was increased with cultural management options. In particular, if integrated cultural management options of allelopathic rice varieties included a low-dose (bensulfuron-methyl, 25 g AI ha(-1), a third of the recommended dose) herbicide application, the emergence and growth of most weeds found in paddy fields was completely controlled. No grain yield reduction for allelopathic varieties occurred under integrated cultural management options, whereas with the non-allelopathic variety a reduction of up to 45-60% was measurable even with the low-dose herbicide application. The allelopathic potential of rice varieties will likely have a great impact on paddy weed control if integrated with cultural management options and application of low doses of herbicides. Therefore, it is feasible to reduce herbicide input in paddies if allelopathic rice is grown under integrated cultural management practices. (c) 2008 Society of Chemical Industry.

Microbial, physical and chemical properties of irrigation water in rice fields of Southern Brazil.

PubMed

Reche, Maria Helena L R; Machado, Vilmar; Saul, Danilo A; Macedo, Vera R M; Marcolin, Elio; Knaak, Neiva; Fiuza, Lidia M

2016-03-01

This paper presents the results of the statistical analysis of microbiological, physical and chemical parameters related to the quality of the water used in rice fields in Southern Brazil. Data were collected during three consecutive crop years, within structure of a comprehensive monitoring program. The indicators used were: potential hydrogen, electrical conductivity, turbidity, nitrogen, phosphorus, potassium, calcium, total and fecal coliforms. Principal Component and Discriminant Analysis showed consistent differences between the water irrigation and drainage, as the temporal variation demonstrated a clear reduction in the concentration of most of the variables analyzed. The pattern of this reduction is not the same in the two regions - that is, the importance of each of the different variables in the observed differentiation is modified in two locations. These results suggested that the variations in the water quality utilized for rice irrigation was influenced by certain specific aspects of each rice region in South Brazilian - such as anthropic action or soil/climate conditions in each hydrographic basin.

Species diversity of ostracods (Crustacea: Ostracoda) from rice fields in Northeast Thailand, with the description of a new Tanycypris species.

PubMed

Savatenalinton, Sukonthip

2017-12-07

The species diversity of ostracods in rice fields from Northeast Thailand was studied. Fifty-two samples were collected from 43 rice fields during 2010-2016. This investigation revealed 23 genera and 52 species, including one new to science (Tanycypris eugenkempfi n. sp.) and 21 endemic species: endemic to the Oriental region (14 species), Southeast Asia (two species) and Thailand (five species). In addition, two species that were restricted to rice fields in this study were recognized: Bradleytriebella tuberculata (Hartmann, 1964) and Notodromas sinensis Neale & Zhao, 1991. The most diverse genus was Pseudostrandesia, followed by Strandesia with eight and five species, respectively. The most widely distributed species were Pseudocypretta maculata Klie, 1932 and Strandesia kraepelini (G.W. Müller, 1906) occurring in 48.8 % of the total of surveyed rice fields. The number of species in individual rice fields ranged from none to 18. Most of the samples contained three species, but one rice field showed very high species richness (18 species). Most of the species recorded in this study were also found in several types of water bodies and reported from several zoogeographical regions. The main distinguishing characters between Tanycypris eugenkempfi n. sp. and other Tanycypris species are the more tumid carapace in dorsal view, the distinctly arched dorsal margin of the carapace in lateral view, the presence of a subapical dorsal seta on the first segment of the first antenna, the smooth large bristles on the third endite of the maxillula, the absence of c-seta on the first thoracopod, and the long proximal claw (Gp) of the caudal ramus.

Identification of large masses of citrus fruit and rice fields in eastern Spain

NASA Technical Reports Server (NTRS)

Desagredo, F. L.; Salinas, F. G.

1973-01-01

ERTS-1 imagery has been successfully used for the identification of large areas of citrus groves and rice fields in the Valencia region of Eastern Spain. Results are encouraging and will facilitate the elaboration of a land use map with a fair degree of definition once methods prove to be fully operational.

[Distribution characteristics of soil profile nitrous oxide concentration in paddy fields with different rice-upland crop rotation systems].

PubMed

Liu, Ping-li; Zhang, Xiao-lin; Xiong, Zheng-qin; Huang, Tai-qing; Ding, Min; Wang, Jin-yang

2011-09-01

To investigate the dynamic distribution patterns of nitrous oxide (N2O) in the soil profiles in paddy fields with different rice-upland crop rotation systems, a special soil gas collection device was adopted to monitor the dynamics of N2O at the soil depths 7, 15, 30, and 50 cm in the paddy fields under both flooding and drainage conditions. Two rotation systems were installed, i.e., wheat-single rice and oilseed rape-double rice, each with or without nitrogen (N) application. Comparing with the control, N application promoted the N2O production in the soil profiles significantly (P < 0.01), and there existed significant correlations in the N2O concentration among the four soil depths during the whole observation period (P < 0.01). In the growth seasons of winter wheat and oilseed rape under drainage condition and with or without N application, the N2O concentrations at the soil depths 30 cm and 50 cm were significantly higher than those at the soil depths 7 cm and 15 cm; whereas in the early rice growth season under flooding condition and without N application, the N2O concentrations at the soil depth 7 cm and 15 cm were significantly higher than those at the soil depths 30 cm and 50 cm (P < 0.05). No significant differences were observed in the N2O concentrations at the test soil depths among the other rice cropping treatments. The soil N2O concentrations in the treatments without N application peaked in the transitional period from the upland crops cropping to rice planting, while those in the treatments with N application peaked right after the second topdressing N of upland crops. Relatively high soil N2O concentrations were observed at the transitional period from the upland crops cropping to rice planting.

Association and interaction between dietary pattern and VEGF receptor-2 (VEGFR2) gene polymorphisms on blood lipids in Chinese Malaysian and Japanese adults.

PubMed

Yap, Roseline Wai Kuan; Shidoji, Yoshihiro; Hon, Wei Min; Masaki, Motofumi

2012-01-01

Dietary pattern and genetic predisposition of each population have different impacts on lifestyle-related chronic diseases. This study was conducted to evaluate the association and interaction between dietary patterns and VEGFR2 or KDR gene polymorphisms on physical and biochemical risk factors of cardiovascular disease in two Asian populations (179 Chinese Malaysian and 136 Japanese adults). Dietary patterns were constructed from food frequency questionnaire using factor analysis. Genotyping of rs1870377 and rs2071559 was performed by real-time PCR using TaqMan probes. Physical measurements: body mass index, systolic and diastolic blood pressures and biochemical parameters: glycated hemoglobin A1c and blood lipids (total cholesterol, triglycerides, LDL-cholesterol, HDL-cholesterol and total cholesterol/HDL-C ratio) were measured. Two dietary patterns were extracted for: Japanese ('Japanese diet' and 'Western diet') and Chinese Malaysians ('Balanced diet'; and 'Meat, rice and noodles diet'). In Japanese, 'Western Diet' and rs2071559 were associated with LDL-C and HDL-C, respectively. In Chinese Malaysians, 'Meat, rice and noodles diet' was asso-ciated with triglycerides, HDL-C and total cholesterol/HDL ratio while rs1870377 and rs2071559 were associated with total cholesterol and/or LDL-C. The interaction between 'Western Diet' and rs2071559 in Japanese and 'Meat, rice and noodles diet' and rs1870377 in Malaysians had significant effects on blood lipids after adjusting for confounders. The association and interaction of dietary patterns and VEGFR2 gene polymorphisms on blood lipids differ between Chinese Malaysian and Japanese subjects by either decreasing or increasing the risk of cardiovascular disease.

Improving water management practices to reduce nutrient export from rice paddy fields.

PubMed

Zhang, Zhi-Jian; Yao, Ju-Xiang; Wang, Zhao-De; Xu, Xin; Lin, Xian-Yong; Czapar, George F; Zhang, Jian-Ying

2011-01-01

Nitrogen (N) and phosphorus (P) loss from rice paddy fields represents a significant threat to water quality in China. In this project, three irrigation-drainage regimes were compared, including one conventional irrigation-drainage regime, i.e. continuous submergence regime (CSR), and two improved regimes, i.e. the alternating submergence-nonsubmergence regime (ASNR) and the zero-drainage irrigation technology (ZDIT), to seek cost-effective practices for reducing nutrient loss. The data from these comparisons showed that, excluding the nutrient input from irrigation, the net exports of total N and total P via surface field drainage ranged from -3.93 to 2.39 kg ha and 0.17 to 0.95 g ha(-1) under the CSR operation, respectively, while N loss was -2.46 to -2.23 kg ha(-1) and P export was -0.65 to 0.31 kg ha(-1) under the improved regimes. The intensity of P export was positively correlated to the rate of P application. Reducing the draining frequency or postponing the draining operation would shift the ecological role of the paddy field from a nutrient export source to an interception sink when ASNR or the zero-drainage water management was used. In addition, since the rice yields are being guaranteed at no additional cost, the improved irrigation-drainage operations would have economic as well as environmental benefits.

The ambient stress field in the continental margin around the Korean Peninsula and Japanese islands

NASA Astrophysics Data System (ADS)

Lee, J.; Hong, T. K.; Chang, C.

2016-12-01

The ambient stress field is mainly influenced by regional tectonics. The stress field composition is crucial information for seismic hazard assessment. The Korean Peninsula, Japanese Islands and East Sea comprise the eastern margin of the Eurasian plate. The regions are surrounded by the Okhotsk, Pacific, and Philippine Sea plates. We investigate the regional stress field around the Korean Peninsula and Japanese islands using the focal mechanism solutions of regional earthquakes. Complex lateral and vertical variations of regional crustal stress fields are observed around a continental margin. The dominant compression directions are ENE-WSW around the Korean Peninsula and eastern China, E-W in the central East Sea and northern and southern Japan, NW-SE in the central Japan, and N-S around the northern Nankai trough. The horizontal compression directions are observed to be different by fault type, suggesting structure-dependent stress field distortion. The regional stress field change by depth and location, suggesting that the compression and tension stress may alternate in local region. The stress field and structures affect mutually, causing stress field distortion and reactivation of paleo-structures. These observation may be useful for understanding of local stress-field perturbation for seismic hazard mitigation of the region.

Natto and viscous vegetables in a Japanese style meal suppress postprandial glucose and insulin responses.

PubMed

Taniguchi, Akiko; Yamanaka-Okumura, Hisami; Nishida, Yuka; Yamamoto, Hironori; Taketani, Yutaka; Takeda, Eiji

2008-01-01

Naturally viscous vegetables and natto, made by fermenting soybeans, are very palatable and considered to be healthy foods in Japan. The objective was to assess whether the consumption of natto and viscous vegetables as part of a traditional Japanese breakfast based on high-glycemic index white rice affects glycemic, insulinemic, lipidemic and satiety responses in healthy subjects. Eleven healthy subjects consumed the reference, control and test meals in a randomized cross-over design. The test meal, comprising 200 g of boiled white rice with viscous meal (50 g natto, 60 g Japanese yams and 40 g okras), and the control meal, comprising 200 g of white rice with non-viscous boiled soybeans, potatoes and broccoli, contained comparable amounts of carbohydrate, fat, protein and fiber. In addition, whiter rice was used as a reference meal. Blood samples over 180 min were analyzed for glucose, insulin, non-esterified free fatty acid and triacylglyceride. Peak glucose and insulin concentrations after the test meal (6.0 mmol/L and 262 pmol/L) were significantly lower than after the control meal (6.8 mmol/L and 360 pmol/L). The incremental areas under the curve for glucose and insulin over 0-120 min after the test meal were also significantly reduced as compared with the control meal (28 and 27%). The consumption of naturally viscous vegetables with white rice reduced acute glycemia and insulinemia. This practical dietary combination would ensure compliance and favorably alter the risk for diabetes and cardiovascular diseases.

Waste rice for waterfowl in the Mississippi Alluvial Valley

USGS Publications Warehouse

Stafford, J.D.; Kaminski, R.M.; Reinecke, K.J.; Manley, S.W.

2006-01-01

Flooded rice fields are important foraging habitats for waterfowl in the lower Mississippi Alluvial Valley (MAV). Waste rice previously was abundant in late autumn (140?492 kg/ha), but early planting and harvest dates in recent years may have increased losses of waste rice during autumn before waterfowl arrive. Research in Mississippi rice fields revealed waste-rice abundance decreased 79?99% during autumns 1995?1996. To determine if this trend existed throughout the MAV, we used multistage sampling (MSS) to estimate waste-rice abundance during September?December 2000?2002. Averaged over years, mean abundance of waste rice decreased 71% between harvest ((x) over bar = 271.0 kg/ha, CV = 13% n = 3 years) and late autumn ( (x) over bar = 78.4 kg/ha, CV = 15% n = 3). Among 15 models formulated to explain variation in rice abundance among fields and across years, the best model indicated abundance of waste rice in late autumn differed between harvester types (i.e., conventional > stripper header) and was positively related to initial waste-rice abundance after harvest. Because abundance of waste rice in late autumn was less than previous estimates in all 3 years, we concluded that waterfowl conservationists have overestimated carrying capacity of rice fields for wintering waterfowl by 52?83% and recommend 325 duck-use days/ha (DUDs) as a revised estimate. We suggest monitoring advances in rice harvest dates to determine when new surveys are warranted and recommend increased management of moist-soil wetlands to compensate for decreased rice abundance.

Improved stress tolerance and productivity in transgenic rice plants constitutively expressing the Oryza sativa glutathione synthetase OsGS under paddy field conditions.

PubMed

Park, Seong-Im; Kim, Young-Saeng; Kim, Jin-Ju; Mok, Ji-Eun; Kim, Yul-Ho; Park, Hyang-Mi; Kim, Il-Sup; Yoon, Ho-Sung

2017-08-01

Reactive oxygen species, which increase under various environmental stresses, have deleterious effects on plants. An important antioxidant, glutathione, is used to detoxify reactive oxygen species in plant cells and is mainly produced by two enzymes: gamma-glutamylcysteine synthetase (γ-ECS) and glutathione synthetase (GS). To evaluate the functional roles of the glutathione synthetase gene (OsGS) in rice, we generated four independent transgenic rice plants (TG1-TG4) that overexpressed OsGS under the control of the constitutively expressed OsCc1 promoter. When grown under natural paddy field conditions, the TG rice plants exhibited greater growth development, higher chlorophyll content, and higher GSH/GSSH ratios than control wild-type (WT) rice plants. Subsequently, the TG rice plants enhanced redox homeostasis by preventing hydroperoxide-mediated membrane damage, which improved their adaptation to environmental stresses. As a result, TG rice plants improved rice grain yield and total biomass following increases in panicle number and number of spikelets per panicle, despite differences in climate during the cultivation periods of 2014 and 2015. Overall, our results indicate that OsGS overexpression improved redox homeostasis by enhancing the glutathione pool, which resulted in greater tolerance to environmental stresses in the paddy fields. Copyright © 2017. Published by Elsevier GmbH.

Utilizing NASA Earth Observations to Monitor Land Management Practices and the Development of Marshlands to Rice Fields in Rwanda

NASA Astrophysics Data System (ADS)

Dusabimana, M. R.; Blach, D.; Mwiza, F.; Muzungu, E.; Swaminathan, R.; Tate, Z.

2014-12-01

Rwanda, a small country with the highest population density in Sub-Saharan Africa, is one of the world's poorest countries. Although agriculture is the backbone of Rwandan economy, agricultural productivity is extremely low. Over 90 % of the population is engaged in subsistence farming and only 52 % of the total land surface area is arable. Of this land, approximately 165,000 hectares are marshlands, of which only 57 % has been cultivated. Rwandan government has invested in the advancement of agriculture with activities such as irrigation, marshland reclamation, and crop regionalization. In 2001, Ministry of Agriculture and Animal Resources (MINAGRI) released the Rural Sector Support Program (RSSP), which aimed at converting marshlands into rice fields at various development sites across the country. The focus of this project was to monitor rice fields in Rwanda utilizing NASA Earth observations such as Landsat 5 Thematic Mapper and Landsat 8 Operational Land Imager. Modified Normalized Difference Water Index (MNDWI) was used to depict the progress of marshland to rice field conversion as it highlights the presence of irrigated rice fields from the surrounding area. Additionally, Decision Support System for Agrotechnology Transfer (DSSAT) was used to estimate rice yield at RSSP sites. Various simulations were run to find perfect conditions for cultivating the highest yield for a given farm. Furthermore, soil erosion susceptibility masks were created by combining factors derived from ASTER, MERRA, and ground truth data using Revised Universal Soil Loss Equation (RUSLE). The end results, maps, and tutorials were delivered to the partners and policy makers in Rwanda to help make informed decisions. It can be clearly seen that Earth observations can be successfully used to monitor agricultural and land management practices as a cost effective method that will enable farmers to improve crop yield production and food security.

Aquaporins are major determinants of water use efficiency of rice plants in the field.

PubMed

Nada, Reham M; Abogadallah, Gaber M

2014-10-01

This study aimed at specifying the reasons of unbalanced water relations of rice in the field at midday which results in slowing down photosynthesis and reducing water use efficiency (WUE) in japonica and indica rice under well-watered and droughted conditions. Leaf relative water content (RWC) decreased in the well-watered plants at midday in the field, but more dramatically in the droughted indica (75.6 and 71.4%) than japonica cultivars (85.5 and 80.8%). Gas exchange was measured at three points during the day (9:00, 13:00 and 17:00). Leaf internal CO2 (Ci) was not depleted when midday stomatal depression was highest indicating that Ci was not limiting to photosynthesis. Most aquaporins were predominantly expressed in leaves suggesting higher water permeability in leaves than in roots. The expression of leaf aquaporins was further induced by drought at 9:00 without comparable responses in roots. The data suggest that aquaporin expression in the root endodermis was limiting to water uptake. Upon removal of the radial barriers to water flow in roots, transpiration increased instantly and photosynthesis increased after 4h resulting in increasing WUE after 4h, demonstrating that WUE in rice is largely limited by the inadequate aquaporin expression profiles in roots. Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

Can herbicide safeners allow selective control of weedy rice infesting rice crops?

PubMed

Busi, Roberto; Nguyen, Nghia K; Chauhan, Bhagirath S; Vidotto, Francesco; Tabacchi, Maurizio; Powles, Stephen B

2017-01-01

Rice is a major field crop of paramount importance for global food security. However, the increased adoption of more profitable and resource-efficient direct-seeded rice (DSR) systems has contributed to greater weed infestations, including weedy rice, which has become a severe problem in several Asian regions. In this study we have developed a conceptually novel method to protect rice plants at high doses of clomazone and triallate. The insecticide phorate applied to rice seeds provided a substantial level of protection against the herbicides clomazone or triallate. A quantity of 15 kg phorate ha -1 significantly increased the LD 50 values, which were more than twofold greater than for rice plants treated only with clomazone. A quantity of 20 kg phorate ha -1 in combination with 2000 g triallate ha -1 safened rice plants (80% survival) with LD 50 >3.4-fold greater than in phorate-untreated rice. Weed control efficacy was not lowered by the presence of phorate-treated rice seeds. Weedy rice is one of the most damaging global weeds and a major threat to DSR systems. In this study we have developed a proof-of-concept method to allow selective weedy rice control in rice crops. We call for herbicide discovery programmes and research to identify candidate safener and herbicide combinations to achieve selective herbicide control of weedy rice and alleviate weed infestations in global rice crops. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry.

Phenotypic and molecular characterisation of efficient nitrogen-fixing Azotobacter strains from rice fields for crop improvement.

PubMed

Sahoo, Ranjan K; Ansari, Mohammad W; Dangar, Tushar K; Mohanty, Santanu; Tuteja, Narendra

2014-05-01

Biological nitrogen fixation (BNF) is highly effective in the field and potentially useful to reduce adverse effects chemical fertilisers. Here, Azotobacter species were selected via phenotypic, biochemical and molecular characterisations from different rice fields. Acetylene reduction assay of Azotobacter spp. showed that Azotobacter vinelandii (Az3) fixed higher amount of nitrogen (121.09 nmol C2H4 mg(-1) bacteria h(-1)). Likewise, its plant growth functions, viz. siderophore, hydrogen cyanide, salicylic acid, IAA, GA3, zeatin, NH3, phosphorus solubilisation, ACC deaminase and iron tolerance, were also higher. The profile of gDNA, plasmid DNA and cellular protein profile depicted inter-generic and inter-specific diversity among the isolates of A. vinelandii. The PCR-amplified genes nifH, nifD and nifK of 0.87, 1.4 and 1.5 kb , respectively, were ascertained by Southern blot hybridisation in isolates of A. vinelandii. The 16S rRNA sequence from A. vinelandii (Az3) was novel, and its accession number (JQ796077) was received from NCBI data base. Biofertiliser formulation of novel A. vinelandii isolates along with commercial one was evaluated in rice (Oriza sativa L. var. Khandagiri) fields. The present finding revealed that treatment T4 (Az3) (A. vinelandii) are highly efficient to improved growth and yield of rice crop.

Impact of Near-Field, Deep-Ocean Tsunami Observations on Forecasting the 7 December 2012 Japanese Tsunami

NASA Astrophysics Data System (ADS)

Bernard, Eddie; Wei, Yong; Tang, Liujuan; Titov, Vasily

2014-12-01

Following the devastating 11 March 2011 tsunami, two deep-ocean assessment and reporting of tsunamis (DART®)(DART® and the DART® logo are registered trademarks of the National Oceanic and Atmospheric Administration, used with permission) stations were deployed in Japanese waters by the Japanese Meteorological Agency. Two weeks after deployment, on 7 December 2012, a M w 7.3 earthquake off Japan's Pacific coastline generated a tsunami. The tsunami was recorded at the two Japanese DARTs as early as 11 min after the earthquake origin time, which set a record as the fastest tsunami detecting time at a DART station. These data, along with those recorded at other DARTs, were used to derive a tsunami source using the National Oceanic and Atmospheric Administration tsunami forecast system. The results of our analysis show that data provided by the two near-field Japanese DARTs can not only improve the forecast speed but also the forecast accuracy at the Japanese tide gauge stations. This study provides important guidelines for early detection and forecasting of local tsunamis.

Insect remote sensing using a polarization sensitive cw lidar system in chinese rice fields

NASA Astrophysics Data System (ADS)

Zhu, Shiming; Malmqvist, Elin; Li, Yiyun; Jansson, Samuel; Li, Wansha; Duan, Zheng; Fu, Wei; Svanberg, Katarina; Bood, Joakim; Feng, Hongqiang; Åkesson, Susanne; Song, Ziwei; Zhang, Baoxin; Zhao, Guangyu; Li, Dunsong; Brydegaard, Mikkel; Svanberg, Sune

2018-04-01

A joint Chinese-Swedish field campaign of Scheimpflug continuous-wave lidar monitoring of rice-field flying pest insects was pursued in very hot July weather conditions close to Guangzhou, China. The occurrence of insects, birds and bats with almost 200 hours of round-the-clock polarization-sensitive recordings was studied. Wing-beat frequency recordings and depolarization properties were used for target classification. Influence of weather conditions on the flying fauna was also investigated.

Evidence for the emergence of new rice types of interspecific hybrid origin in West African farmers' fields.

PubMed

Nuijten, Edwin; van Treuren, Robbert; Struik, Paul C; Mokuwa, Alfred; Okry, Florent; Teeken, Béla; Richards, Paul

2009-10-06

In West Africa two rice species (Oryza glaberrima Steud. and Oryza sativa L.) co-exist. Although originally it was thought that interspecific hybridization is impossible without biotechnological methods, progenies of hybridization appear to occur in farmer fields. AFLP analysis was used to assess genetic diversity in West Africa (including the countries The Gambia, Senegal, Guinea Bissau, Guinea Conakry, Sierra Leone, Ghana and Togo) using 315 rice samples morphologically classified prior to analysis. We show evidence for farmer interspecific hybrids of African and Asian rice, resulting in a group of novel genotypes, and identify possible mechanisms for in-field hybridization. Spontaneous back-crossing events play a crucial role, resulting in different groups of genetic diversity in different regions developed by natural and cultural selection, often under adverse conditions. These new groups of genotypes may have potential relevance for exploitation by plant breeders. Future advances in crop development could be achieved through co-operation between scientists and marginalized farmer groups in order to address challenges of rapid adaptation in a world of increasing socio-political and climatic uncertainty.

Evidence for the Emergence of New Rice Types of Interspecific Hybrid Origin in West African Farmers' Fields

PubMed Central

Nuijten, Edwin; van Treuren, Robbert; Struik, Paul C.; Mokuwa, Alfred; Okry, Florent; Teeken, Béla; Richards, Paul

2009-01-01

In West Africa two rice species (Oryza glaberrima Steud. and Oryza sativa L.) co-exist. Although originally it was thought that interspecific hybridization is impossible without biotechnological methods, progenies of hybridization appear to occur in farmer fields. AFLP analysis was used to assess genetic diversity in West Africa (including the countries The Gambia, Senegal, Guinea Bissau, Guinea Conakry, Sierra Leone, Ghana and Togo) using 315 rice samples morphologically classified prior to analysis. We show evidence for farmer interspecific hybrids of African and Asian rice, resulting in a group of novel genotypes, and identify possible mechanisms for in-field hybridization. Spontaneous back-crossing events play a crucial role, resulting in different groups of genetic diversity in different regions developed by natural and cultural selection, often under adverse conditions. These new groups of genotypes may have potential relevance for exploitation by plant breeders. Future advances in crop development could be achieved through co-operation between scientists and marginalized farmer groups in order to address challenges of rapid adaptation in a world of increasing socio-political and climatic uncertainty. PMID:19806197

Comparative Analysis of the Genomes of Two Field Isolates of the Rice Blast Fungus Magnaporthe oryzae

PubMed Central

Li, Zhigang; Hu, Songnian; Yao, Nan; Dean, Ralph A.; Zhao, Wensheng; Shen, Mi; Zhang, Haiwang; Li, Chao; Liu, Liyuan; Cao, Lei; Xu, Xiaowen; Xing, Yunfei; Hsiang, Tom; Zhang, Ziding; Xu, Jin-Rong; Peng, You-Liang

2012-01-01

Rice blast caused by Magnaporthe oryzae is one of the most destructive diseases of rice worldwide. The fungal pathogen is notorious for its ability to overcome host resistance. To better understand its genetic variation in nature, we sequenced the genomes of two field isolates, Y34 and P131. In comparison with the previously sequenced laboratory strain 70-15, both field isolates had a similar genome size but slightly more genes. Sequences from the field isolates were used to improve genome assembly and gene prediction of 70-15. Although the overall genome structure is similar, a number of gene families that are likely involved in plant-fungal interactions are expanded in the field isolates. Genome-wide analysis on asynonymous to synonymous nucleotide substitution rates revealed that many infection-related genes underwent diversifying selection. The field isolates also have hundreds of isolate-specific genes and a number of isolate-specific gene duplication events. Functional characterization of randomly selected isolate-specific genes revealed that they play diverse roles, some of which affect virulence. Furthermore, each genome contains thousands of loci of transposon-like elements, but less than 30% of them are conserved among different isolates, suggesting active transposition events in M. oryzae. A total of approximately 200 genes were disrupted in these three strains by transposable elements. Interestingly, transposon-like elements tend to be associated with isolate-specific or duplicated sequences. Overall, our results indicate that gain or loss of unique genes, DNA duplication, gene family expansion, and frequent translocation of transposon-like elements are important factors in genome variation of the rice blast fungus. PMID:22876203

Establishment of a rice-duck integrated farming system and its effects on soil fertility and rice disease control

NASA Astrophysics Data System (ADS)

Teng, Qing; Hu, Xue-Feng; Cheng, Chang; Luo, Zhi-Qing; Luo, Fan

2015-04-01

Rice-duck integrated farming is an ecological farming system newly established in some areas of southern China . It was reported that the ducks walking around the paddy fields is beneficial to control weed hazards and reduce rice pests and diseases. To study and evaluate the effects of the rice-duck integrated farming on soil fertility and rice disease control, a field experiment of rice cultivation was carried out in the suburb of Shanghai in 2014. It includes a treatment of raising ducks in the fields and a control without ducks. The treatment was implemented by building a duck coop nearby the experimental fields and driving 15 ducks into a plot at daytime since the early stage of rice growth. Each plot is 667 m2 in area. The treatment and control were replicated for three times. No any herbicides, pesticides, fungicides and chemical fertilizers were applied during the experiment to prevent any disturbance to duck growing and rice weed hazards and disease incidences from agrochemicals. The results are as follows: (1) The incidences of rice leaf rollers (Cnaphalocrocis medinalis) and stem borers treated with ducks, 0.45%and 1.18% on average, respectively, are lower than those of the control, 0.74% and 1.44% on average, respectively. At the late stage of rice growth, the incidence of rice sheath blight treated with ducks, 13.15% on average, is significantly lower than that of the control, 16.9% on average; and the incidence of rice planthoppers treated with ducks, 11.3 per hill on average, is also significantly lower than that of the control, 47.4 per hill on average. (2) The number of weeds in the plots treated with ducks, 8.3 per m2 on average, is significantly lower than that of the control, 87.5 m2 on average. (3) Raising ducks in the fields could also enhance soil enzyme activity and nutrient status. At the late stage of rice growth, the activities of urease, phosphatase, sucrase and catalase in the soils treated with ducks are 1.39 times, 1.40 times, 1

Effect of transgenic Bacillus thuringiensis rice lines on mortality and feeding behavior of rice stem borers (Lepidoptera: Crambidae).

PubMed

Chen, Hao; Zhang, Guoan; Zhang, Qifa; Lin, Yongjun

2008-02-01

Ten transgenic Bacillus thuringiensis Bt rice, Oryza sativa L., lines with different Bt genes (two Cry1Ac lines, three Cry2A lines, and five Cry9C lines) derived from the same variety Minghui 63 were evaluated in both the laboratory and the field. Bioassays were conducted by using the first instars of two main rice lepidopteran insect species: yellow stem borer, Scirpophaga incertulas (Walker) and Asiatic rice borer, Chilo suppressalis (Walker). All transgenic lines exhibited high toxicity to these two rice borers. Field evaluation results also showed that all transgenic lines were highly insect resistant with both natural infestation and manual infestation of the neonate larvae of S. incertulas compared with the nontransformed Minghui63. Bt protein concentrations in leaves of 10 transgenic rice lines were estimated by the sandwich enzyme-linked immunosorbent assay. The cry9C gene had the highest expression level, next was cry2A gene, and the cry1Ac gene expressed at the lowest level. The feeding behavior of 7-d-old Asiatic rice borer to three classes of Bt transgenic rice lines also was detected by using rice culm cuttings. The results showed that 7-d-old larvae of Asiatic rice borer have the capacity to distinguish Bt and non-Bt culm cuttings and preferentially fed on non-Bt cuttings. When only Bt culm cuttings with three classes of different Bt proteins (CrylAc, Cry2A, and Cry9C) were fed, significant distribution difference of 7-d-old Asiatic rice borer in culm cuttings of different Bt proteins also was found. In the current study, we evaluate different Bt genes in the same rice variety in both the laboratory and the field, and also tested feeding behavior of rice insect to these Bt rice. These data are valuable for the further development of two-toxin Bt rice and establishment of appropriate insect resistance management in the future.

[Influence of paddy rice-upland crop rotation of cold-waterlogged paddy field on crops produc- tion and soil characteristics].

PubMed

Wang, Fei; Li, Qing-hua; Lin, Cheng; He, Chun-mei; Zhong, Shao-jie; Li, Yu; Lin, Xin-jian; Huang, Jian-cheng

2015-05-01

Two consecutive years (4-crop) experiments were conducted to study the influence of different paddy rice-upland crop rotation in cold-waterlogged paddy field on the growth of crops and soil characteristics. The result showed that compared with the rice-winter fallow (CK) pattern, the two-year average yield of paddy rice under four rotation modes, including rape-rice (R-R), spring corn-rice (C-R), Chinese milk vetch-rice (M-R) and bean-rice (B-R), were increased by 5.3%-26.7%, with significant difference observed in C-R and R-R patterns. Except for M-R pattern, the annual average total economic benefits were improved by 79.0%-392.4% in all rotation pattern compared with the CK, and the ration of output/input was enhanced by 0.06-0.72 unit, with the most significant effect found in the C-R pattern. Likewise, compared with the CK, the contents of chlorophyll and carotenoid, and net photosynthetic rate (Pn) of rice plant were all increased during the full-tillering stage of rice in all rotation patterns. The rusty lines and rusty spots of soils were more obvious compared with the CK during the rice harvest, particularly in R-R, C-R and B-R patterns. The ratio of water-stable soil macro aggregates of plough layer of soil (> 2 mm) decreased at different levels in all rotation patterns while the ratios of middle aggregate (0.25-2 mm, expect for M-R) and micro aggregate of soil (< 0.25 mm) were opposite. There was a decreasing trend for soil active reducing agents in all rotation patterns, whereas the available nutrient increased. The amounts of soil bacteria in C-R and B-R patterns, fungi in B-R rotation pattern, cellulose bacteria in R-R, C-R and B-R patterns and N-fixing bacteria in B-R pattern were improved by 285.7%-403.0%, 221.7%, 64.6-92.2% and 162.2%, respectively. Moreover, the differences in all microorganisms were significant. Thus, based on the experimental results of cold-waterlogged paddy field, it was concluded that changing from single cropping rice system

Nitrogen and phosphorus effluent loads from a paddy-field district adopting collective crop rotation.

PubMed

Hama, T; Aoki, T; Osuga, K; Sugiyama, S; Iwasaki, D

2012-01-01

Japanese paddy rice systems commonly adopt the rotation of vegetables, wheat and soybeans with paddy rice. Crop rotation may, however, increase the nutrient load in effluent discharged from the district because more fertilizer is applied to the rotation crops than is applied to paddy crops. We investigated a paddy-field district subject to collective crop rotation and quantified the annual nutrient load of effluent from the district in three consecutive years. The total annual exports of nitrogen and phosphorus over the investigation period ranged from 30.3 to 40.6 kg N ha(-1) and 2.62 to 3.13 kg P ha(-1). The results suggest that rotation cropping increases the effluent nutrient load because applied fertilizer is converted to nitrate, and surface runoff is increased due to the absence of shuttering boards at the field outlets.

Role of Remote Sensing and Geographyc Information System Mapping for Protected Areas Land Rice Field Subak, Buffer Zones, and Area Conversion (Case Studies In Gianyar Regency, Bali Province)

NASA Astrophysics Data System (ADS)

Lanya, Indayati; Netera Subadiyasa, N.

2016-11-01

Conversion of rice fields in Bali 2579 ha/year, Law Number 41 of 2009 [1] and five of Government Regulation (GR), mandates the Local Government (LG) has a Regional Regulation (RR) or Rule Regent/Mayor, on the protection of agricultural land sustainable food (PALSF). Yet none provincial government of Bali has PALSF; although Subak as world cultural heritage. Similarly, Gianyar regency development strategy directed to integrate agriculture with tourism. Landsat 8 images, Word View Coverage 2015 Gianyar district and ArcGIS 10.3 software used for of rice field mapping and zoning of land protection Subak. Ten thematic maps (watersheds, land use, irrigation, relief/slope, rainfall, spatial planning, land suitability, productivity, the distance from downtown) as a variable parameter, weighted and balanced numerically. Numerical classification agricultura land using for the overlay menu and reselek. The total value of >125 as rice need to be protected, 100-125 value for buffer zone, and the value of <100 rice fields can be converted to 40 next year. For the 20 next year, the total value of> 100, 50-100 and <50 respectively to rice fields that need to be protected, wetland buffer, and rice fields can be converted. Region Subak sustainable of rice field protection, buffer and can be converted in a row for the next 20 years is 10973 ha, 3855 ha and 311 ha. For the next 40 years Subak conserved of rice field (8019 ha), buffer (5855 ha), and can be converted (3124 ha). Subak land pattern of spread can be converted to an supply of land for non-agricultural development of the region downstream to the access road Ida Bagus Matera (Jln. Province / national) in the coastal areas of Gianyar.

Forms of trace arsenic, cesium, cadmium, and lead transported into river water for the irrigation of Japanese paddy rice fields

NASA Astrophysics Data System (ADS)

Nakaya, Shinji; Chi, Hai; Muroda, Kengo; Masuda, Harue

2018-06-01

In this study, we focus on the behavior of geogenic, toxic trace elements, particularly As, Cs, Cd, and Pb, during their transportation in two rivers for irrigation commonly used in monsoon Asia; one river originates from an active volcano, Mt. Asama, and the other originates from a currently inactive volcano, Yatsugatake Mountains in Nagano, Japan. These rivers were investigated to understand the role of river water as a pollutant of rice and other aquatic plants (via irrigation) and aquatic animals. The results indicated that the behavior of toxic trace elements in river water are likely controlled by their interactions with particulate Fe, Al, and Ti compounds. The majority of Pb and Cd is transported as particulate matter with Fe, Al, and Ti, while the majority of As is transported in the dissolved form, predominantly as arsenate, with low abundance of particulate matter. Cs is transported either as the dissolved form or as particulate matter in both rivers. The investigated elements are transported in the rivers as particulate and dissolved forms, and the ratio of these forms is controlled by the pH and presence of particulate Fe, Al, and Ti phases in the river water. With respect to Cs in both rivers, the parameter governing the concentration and transportation of Cs, in the bimodal form (i.e., particulate and dissolved forms), through the river possibly shifts from sorption to pH by particulate Fe-Al-Ti, according to the abrupt increase in the concentration of Cs in the river. The chemical attraction of particulate Fe-Al-Ti for Cs is weaker than that for Pb and Cd, indicating that the lower electronegativity of Cs weakens the chemical attraction on a colloid for the competitive sorption with the other trace elements. The different relationships between As and Fe in the river and in the irrigation water and soil water, as well as those in paddy rice, suggested that As in paddy rice is not directly derived from As in the irrigation water from the river under

Field experiment for determining lead accumulation in rice grains of different genotypes and correlation with iron oxides deposited on rhizosphere soil.

PubMed

Lai, Yu-Cheng; Syu, Chien-Hui; Wang, Pin-Jie; Lee, Dar-Yuan; Fan, Chihhao; Juang, Kai-Wei

2018-01-01

Paddy rice (Oryza sativa L.) is a major staple crop in Asia. However, heavy metal accumulation in paddy soil poses a health risk for rice consumption. Although plant uptake of Pb is usually low, Pb concentrations in rice plants have been increasing with Pb contamination in paddy fields. It is known that iron oxide deposits in the rhizosphere influence the absorption of soil Pb by rice plants. In this study, 14 rice cultivars bred in Taiwan, including ten japonica cultivars (HL21, KH145, TC192, TK9, TK14, TK16, TN11, TNG71, TNG84, and TY3) and four indica cultivars (TCS10, TCS17, TCSW2, and TNGS22), were used in a field experiment. We investigated the genotypic variation in rice plant Pb in relation to iron oxides deposited in the rhizosphere, as seen in a suspiciously contaminated site in central Taiwan. The results showed that the cultivars TCSW2, TN11, TNG71, and TNG84 accumulated brown rice Pb exceeding the tolerable level of 0.2mgkg -1 . In contrast, the cultivars TNGS22, TK9, TK14, and TY3 accumulated much lower brown rice Pb (<0.1mgkg -1 ); therefore, they should be prioritized as safe cultivars for sites with potential contamination. Moreover, the iron oxides deposited on the rhizosphere soil show stronger affinity to soil-available Pb than those on the root surface to form iron plaque. The relative tendency of Pb sequestration toward rhizosphere soil was negatively correlated with the Pb concentrations in brown rice. The iron oxides deposited on the rhizosphere soil but not on the root surface to form iron plaque dominate Pb sequestration in the rhizosphere. Therefore, the enhancement of iron oxide deposits on the rhizosphere soil could serve as a barrier preventing soil Pb on the root surface and result in reduced Pb accumulation in brown rice. Copyright © 2017 Elsevier B.V. All rights reserved.

Mapping paddy rice planting area in rice-wetland coexistent areas through analysis of Landsat 8 OLI and MODIS images

PubMed Central

Zhou, Yuting; Xiao, Xiangming; Qin, Yuanwei; Dong, Jinwei; Zhang, Geli; Kou, Weili; Jin, Cui; Wang, Jie; Li, Xiangping

2016-01-01

Accurate and up-to-date information on the spatial distribution of paddy rice fields is necessary for the studies of trace gas emissions, water source management, and food security. The phenology-based paddy rice mapping algorithm, which identifies the unique flooding stage of paddy rice, has been widely used. However, identification and mapping of paddy rice in rice-wetland coexistent areas is still a challenging task. In this study, we found that the flooding/transplanting periods of paddy rice and natural wetlands were different. The natural wetlands flood earlier and have a shorter duration than paddy rice in the Panjin Plain, a temperate region in China. We used this asynchronous flooding stage to extract the paddy rice planting area from the rice-wetland coexistent area. MODIS Land Surface Temperature (LST) data was used to derive the temperature-defined plant growing season. Landsat 8 OLI imagery was used to detect the flooding signal and then paddy rice was extracted using the difference in flooding stages between paddy rice and natural wetlands. The resultant paddy rice map was evaluated with in-situ ground-truth data and Google Earth images. The estimated overall accuracy and Kappa coefficient were 95% and 0.90, respectively. The spatial pattern of OLI-derived paddy rice map agrees well with the paddy rice layer from the National Land Cover Dataset from 2010 (NLCD-2010). The differences between RiceLandsat and RiceNLCD are in the range of ±20% for most 1-km grid cell. The results of this study demonstrate the potential of the phenology-based paddy rice mapping algorithm, via integrating MODIS and Landsat 8 OLI images, to map paddy rice fields in complex landscapes of paddy rice and natural wetland in the temperate region. PMID:27688742

Mapping paddy rice planting area in rice-wetland coexistent areas through analysis of Landsat 8 OLI and MODIS images.

PubMed

Zhou, Yuting; Xiao, Xiangming; Qin, Yuanwei; Dong, Jinwei; Zhang, Geli; Kou, Weili; Jin, Cui; Wang, Jie; Li, Xiangping

2016-04-01

Accurate and up-to-date information on the spatial distribution of paddy rice fields is necessary for the studies of trace gas emissions, water source management, and food security. The phenology-based paddy rice mapping algorithm, which identifies the unique flooding stage of paddy rice, has been widely used. However, identification and mapping of paddy rice in rice-wetland coexistent areas is still a challenging task. In this study, we found that the flooding/transplanting periods of paddy rice and natural wetlands were different. The natural wetlands flood earlier and have a shorter duration than paddy rice in the Panjin Plain, a temperate region in China. We used this asynchronous flooding stage to extract the paddy rice planting area from the rice-wetland coexistent area. MODIS Land Surface Temperature (LST) data was used to derive the temperature-defined plant growing season. Landsat 8 OLI imagery was used to detect the flooding signal and then paddy rice was extracted using the difference in flooding stages between paddy rice and natural wetlands. The resultant paddy rice map was evaluated with in-situ ground-truth data and Google Earth images. The estimated overall accuracy and Kappa coefficient were 95% and 0.90, respectively. The spatial pattern of OLI-derived paddy rice map agrees well with the paddy rice layer from the National Land Cover Dataset from 2010 (NLCD-2010). The differences between Rice Landsat and Rice NLCD are in the range of ±20% for most 1-km grid cell. The results of this study demonstrate the potential of the phenology-based paddy rice mapping algorithm, via integrating MODIS and Landsat 8 OLI images, to map paddy rice fields in complex landscapes of paddy rice and natural wetland in the temperate region.

Influence of rice whole-crop silage diet on growth performance, carcass and meat characteristics and muscle-related gene expression in Japanese Black steers.

PubMed

Shibata, Masahiro; Hikino, Yasuko; Imanari, Mai; Matsumoto, Kazunori; Yamamoto, Naoyuki

2016-07-01

The present study investigated the influence of a diet largely comprising rice whole-crop silage (rWCS) on growth performance, carcass and meat characteristics, and expression of genes involved in muscle growth of Japanese Black steers. Steers were randomly separated into rWCS-fed (rWCS ad libitum and restricted feeding of concentrate) and concentrate-fed groups. Total digestible nutrient intake and daily gain (DG) decreased in rWCS-fed steers in comparison with concentrate-fed steers, whereas dressed carcass weight and final body weight did not significantly differ between the groups. Decreases in drip loss in the muscle of rWCS-fed steers may be caused by α-tocopherol and β-carotene in muscle. Feeding large amounts of rWCS to steers may maintain quantitative productivity of beef steers equally to a concentrate-based diet, and improve the qualitative productivity. Results of gene expression suggest that activation of skeletal muscle growth in rWCS-fed steers may occur at the late fattening period owing to a decrease in myostatin and increase in myosin heavy chain gene expression. Preadipocyte factor-1 and myostatin genes may be strongly involved in the control of lipid accumulation. This rearing system would allow beef production to switch to rWCS-based diets from concentrate-based diets. © 2015 Japanese Society of Animal Science.

Fipronil application on rice paddy fields reduces densities of common skimmer and scarlet skimmer

PubMed Central

Kasai, Atsushi; Hayashi, Takehiko I.; Ohnishi, Hitoshi; Suzuki, Kazutaka; Hayasaka, Daisuke; Goka, Koichi

2016-01-01

Several reports suggested that rice seedling nursery-box application of some systemic insecticides (neonicotinoids and fipronil) is the cause of the decline in dragonfly species noted since the 1990s in Japan. We conducted paddy mesocosm experiments to investigate the effect of the systemic insecticides clothianidin, fipronil and chlorantraniliprole on rice paddy field biological communities. Concentrations of all insecticides in the paddy water were reduced to the limit of detection within 3 months after application. However, residuals of these insecticides in the paddy soil were detected throughout the experimental period. Plankton species were affected by clothianidin and chlorantraniliprole right after the applications, but they recovered after the concentrations decreased. On the other hand, the effects of fipronil treatment, especially on Odonata, were larger than those of any other treatment. The number of adult dragonflies completing eclosion was severely decreased in the fipronil treatment. These results suggest that the accumulation of these insecticides in paddy soil reduces biodiversity by eliminating dragonfly nymphs, which occupy a high trophic level in paddy fields. PMID:26979488

Characterization of Acetic Acid Bacteria in Traditional Acetic Acid Fermentation of Rice Vinegar (Komesu) and Unpolished Rice Vinegar (Kurosu) Produced in Japan

PubMed Central

Nanda, Kumiko; Taniguchi, Mariko; Ujike, Satoshi; Ishihara, Nobuhiro; Mori, Hirotaka; Ono, Hisayo; Murooka, Yoshikatsu

2001-01-01

Bacterial strains were isolated from samples of Japanese rice vinegar (komesu) and unpolished rice vinegar (kurosu) fermented by the traditional static method. Fermentations have never been inoculated with a pure culture since they were started in 1907. A total of 178 isolates were divided into groups A and B on the basis of enterobacterial repetitive intergenic consensus-PCR and random amplified polymorphic DNA fingerprinting analyses. The 16S ribosomal DNA sequences of strains belonging to each group showed similarities of more than 99% with Acetobacter pasteurianus. Group A strains overwhelmingly dominated all stages of fermentation of both types of vinegar. Our results indicate that appropriate strains of acetic acid bacteria have spontaneously established almost pure cultures during nearly a century of komesu and kurosu fermentation. PMID:11157275

Transgenic Bacillus thuringiensis (Bt) rice is safer to aquatic ecosystems than its non-transgenic counterpart.

PubMed

Li, Guangsheng; Wang, Yongmo; Liu, Biao; Zhang, Guoan

2014-01-01

Rice lines genetically modified with the crystal toxin genes from Bacillus thuringiensis (Bt) have experienced rapid development, with biosafety certificates for two Bt rice lines issued in 2009. There has still been no commercial release of these lines yet due to public concerns about human health and environmental risks. Some studies confirmed that Bt rice was as safe as conventional rice to non-target organisms when pesticides were not applied, however, pesticides are still required in Bt rice to control non-lepidopteran pests. In this study, we assessed the environmental effects of two Bt rice lines expressing either the cry1Ab/1Ac or cry2A genes, respectively, by using zooplanktons as indicator species under normal field management practices using pesticides when required. In the whole rice growing season, non-Bt rice was sprayed 5 times while Bt rice was sprayed 2 times, which ensured both rice achieved a normal yield. Field investigations showed that rice type (Bt and non-Bt) significantly influenced zooplankton abundance and diversity, which were up to 95% and 80% lower in non-Bt rice fields than Bt rice fields. Laboratory rearing showed that water from non-Bt rice fields was significantly less suitable for the survival and reproduction of Daphnia magna and Paramecium caudatum in comparison with water from Bt rice fields. Higher pesticide residues were detected in the water from non-Bt than Bt rice fields, accounting for the bad performance of zooplankton in non-Bt field water. Our results demonstrate that Bt rice is safer to aquatic ecosystems than non-Bt rice, and its commercialization will be beneficial for biodiversity restoration in rice-based ecosystems.

Transgenic Bacillus thuringiensis (Bt) Rice Is Safer to Aquatic Ecosystems than Its Non-Transgenic Counterpart

PubMed Central

Li, Guangsheng; Wang, Yongmo; Liu, Biao; Zhang, Guoan

2014-01-01

Rice lines genetically modified with the crystal toxin genes from Bacillus thuringiensis (Bt) have experienced rapid development, with biosafety certificates for two Bt rice lines issued in 2009. There has still been no commercial release of these lines yet due to public concerns about human health and environmental risks. Some studies confirmed that Bt rice was as safe as conventional rice to non-target organisms when pesticides were not applied, however, pesticides are still required in Bt rice to control non-lepidopteran pests. In this study, we assessed the environmental effects of two Bt rice lines expressing either the cry1Ab/1Ac or cry2A genes, respectively, by using zooplanktons as indicator species under normal field management practices using pesticides when required. In the whole rice growing season, non-Bt rice was sprayed 5 times while Bt rice was sprayed 2 times, which ensured both rice achieved a normal yield. Field investigations showed that rice type (Bt and non-Bt) significantly influenced zooplankton abundance and diversity, which were up to 95% and 80% lower in non-Bt rice fields than Bt rice fields. Laboratory rearing showed that water from non-Bt rice fields was significantly less suitable for the survival and reproduction of Daphnia magna and Paramecium caudatum in comparison with water from Bt rice fields. Higher pesticide residues were detected in the water from non-Bt than Bt rice fields, accounting for the bad performance of zooplankton in non-Bt field water. Our results demonstrate that Bt rice is safer to aquatic ecosystems than non-Bt rice, and its commercialization will be beneficial for biodiversity restoration in rice-based ecosystems. PMID:25105299

Sealing rice field boundaries in Bangladesh: a pilot study demonstrating reductions in water use, arsenic loading to field soils, and methane emissions from irrigation water.

PubMed

Neumann, Rebecca B; Pracht, Lara E; Polizzotto, Matthew L; Badruzzaman, A Borhan M; Ali, M Ashraf

2014-08-19

Irrigation of rice fields in Bangladesh with arsenic-contaminated and methane-rich groundwater loads arsenic into field soils and releases methane into the atmosphere. We tested the water-savings potential of sealing field bunds (raised boundaries around field edges) as a way to mitigate these negative outcomes. We found that, on average, bund sealing reduced seasonal water use by 52 ± 17% and decreased arsenic loading to field soils by 15 ± 4%; greater savings in both water use and arsenic loading were achieved in fields with larger perimeter-to-area ratios (i.e., smaller fields). Our study is the first to quantify emission of methane from irrigation water in Bangladesh, a currently unaccounted-for methane source. Irrigation water applied to unsealed fields at our site emits 18 to 31 g of methane per square-meter of field area per season, potentially doubling the atmospheric input of methane from rice cultivation. Bund sealing reduced the emission of methane from irrigation water by 4 to 19 g/m(2). While the studied outcomes of bund sealing are positive and compelling, widespread implementation of the technique should consider other factors, such as effect on yields, financial costs, and impact on the hydrologic system. We provide an initial and preliminary assessment of these implementation factors.

Microbial diversity on sedimentated rice fields due to coal mining activities in Tenggarong Seberang subdistrict of Kutai Kartanegara

NASA Astrophysics Data System (ADS)

Rosfiansyah; Sopialena

2018-04-01

The results showed that on upland rice fields with sediment found five genus of fungus with number of colonies 4.0 x 103 cfu/g to 9.3 x 104 cfu/g; three bacterial families with number of colonies 7,1 x 104 cfu/g to 2,8 x 105 cfu/g; and five genera of nematodes with the amount of 2.6 x 102/kg of soil to 1.1 x103/kg of soil. In unpolished upland rice fields were found four genus of fungus with colonies of 2.4 x 103 cfu/g to 8.4 x 104 cfu/g, three bacterial families with number of colonies 1.2 x 105 cfu/g to 2.7 x 105 cfu/g and four genera of nematodes with the amount of 9.6 x 102/kg of soil to 1.1 x103/kg of soil. The most common microbes are Aspergillus, Cladosporium, Penicillium, Phytium and Trichoderma (fungi), Achromobacteraceae, Brevibacteriaceae, Micrococcaceae (Bacteria), as well as Dorylaimus, Hemicycliophora, Mononchus, Meloidogyne, Paratrichodorus, Radopholus, Rotylenchulus, Rhabditis, Seinura and Trichodorus (Nematodes). Fungi, bacteria and nematodes have a good role in the process of soil decomposition. The results of soil chemical analysis showed that soil fertility is lower in upland rice fields with sediments compare to those without sediment.

Influence of phosphate and copper on reductive dechlorination of thiobencarb in California rice field soils.

PubMed

Gunasekara, Amrith S; Tenbrook, Patti L; Palumbo, Amanda J; Johnson, Catherine S; Tjeerdema, Ronald S

2005-12-28

The potential for reductive dechlorination of the herbicide thiobencarb (TB) by microbes and its prevention in saturated anaerobic rice field soils was examined in laboratory microcosms. TB is effective in controlling both annual grasses and broadleaf weeds. In anoxic microcosms, TB was effectively degraded within 30 days to its dechlorinated product, deschlorothiobencarb (DTB), in two Sacramento Valley rice field soils. TB dechlorination, and subsequent degradation, followed pseudo-zero- (lag phase) and first-order (degradation phase) kinetics. Logistic regression analysis (r2 > 0.841) produced a half-life (t(1/2)) in nonsterile soils ranging from 10 to 15 days, which was also observed when microcosms were amended with low concentrations (<3 mg L(-1)) of copper (Cu2+; as the fungicides Cu(OH)2 and CuSO4.5H2O). High Cu2+ concentrations (>40 mg L(-1)) were added to the microcosms to determine if copper toxicity to dechlorinating microbes is concentration dependent within the range used. After 30 days, the low-copper-amended soils closely resembled the nonsterile experiments to which no Cu2+ was added while the high-copper-amended microcosms were similar to the sterile experiment. Microcosms were also separately amended with 5.7 g L(-1) phosphate (PO4(2-); as KH2PO4), a nutrient regularly applied to rice fields. Phosphate-amended experiments also showed TB degradation, but no DTB formation, indicating the phosphate played a role, possibly as a microbial inhibitor or an alternative electron acceptor, in limiting the dechlorination of TB. In summary, TB dechlorination was inhibited at high Cu(OH)2, CuSO4.5H2O, and KH2PO4 concentrations.

Migration of rice planthoppers and their vectored re-emerging and novel rice viruses in East Asia

PubMed Central

Otuka, Akira

2013-01-01

This review examines recent studies of the migration of three rice planthoppers, Laodelphax striatellus, Sogatella furcifera, and Nilaparvata lugens, in East Asia. Laodelphax striatellus has recently broken out in Jiangsu province, eastern China. The population density in the province started to increase in the early 2000s and peaked in 2004. In 2005, Rice stripe virus (RSV) viruliferous rate of L. striatellus peaked at 31.3%. Since then, rice stripe disease spread severely across the whole province. Due to the migration of the RSV vectors, the rice stripe disease spread to neighboring countries Japan and Korea. An overseas migration of L. striatellus that occurred in 2008 was analyzed, when a slow-moving cold vortex, a type of low pressure system, reached western Japan from Jiangsu, carrying the insects into Japan. Subsequently the rice stripe diseases struck these areas in Japan severely. In Korea, similar situations occurred in 2009, 2011, and 2012. Their migration sources were also estimated to be in Jiangsu by backward trajectory analysis. Rice black-streaked dwarf virus, whose vector is L. striatellus, has recently re-emerged in eastern China, and the evidence for overseas migrations of the virus, just like the RSV’s migrations, has been given. A method of predicting the overseas migration of L. striatellus has been developed by Japanese, Chinese, and Korean institutes. An evaluation of the prediction showed that this method properly predicted migration events that occurred in East Asia from 2008 to 2011. Southern rice black-streaked dwarf virus (SRBSDV) was first found in Guangdong province. Its vector is S. furcifera. An outbreak of SRBSDV occurred in southern China in 2009 and spread to Vietnam the same year. This disease and virus were also found in Japan in 2010. The epidemic triggered many migration studies to investigate concrete spring-summer migration routes in China, and the addition of migration sources for early arrivals in Guangdong and Guangxi

Changes of Field Incurred Chlorpyrifos and Its Toxic Metabolite Residues in Rice during Food Processing from-RAC-to-Consumption

PubMed Central

Zhang, Zhiyong; Jiang, Wayne W.; Jian, Qiu; Song, Wencheng; Zheng, Zuntao; Wang, Donglan; Liu, Xianjin

2015-01-01

The objectives of this study were to determine the effects of food processing on field incurred residues levels of chlorpyrifos and its metabolite 3,5,6-Trichloro-2-pyridinol (TCP) in rice. The chlorpyrifos and TCP were found to be 1.27 and 0.093 mg kg-1 in straw and 0.41 and 0.073 mg kg-1 in grain, respectively. It is observed that the sunlight for 2 hours does not decrease the chlorpyrifos and TCP residues in grain significantly. Their residues in rice were reduced by up to 50% by hulling. The cooking reduced the chlorpyrifos and TCP in rice to undetectable level (below 0.01 mg kg-1). Processing factors (PFs) of chlorpyrifos and TCP residues in rice during food processing were similar. Various factors have impacts on the fates of chlorpyrifos and TCP residues and the important steps to reduce their residues in rice were hulling and cooking. The results can contribute to assure the consumer of a safe wholesome food supply. PMID:25608031

Rice production in relation to soil quality under different rice-based cropping systems

NASA Astrophysics Data System (ADS)

Tran Ba, Linh; Sleutel, Steven; Nguyen Van, Qui; Thi, Guong Vo; Le Van, Khoa; Cornelis, Wim

2016-04-01

Soil quality of shallow paddy soils may be improved by introducing upland crops and thus a more diverse crop cultivation pattern. Yet, the causal relationship between crop performance and enhanced soil traits in rice-upland crop rotations remains elusive. The objectives of this study were to (i) find correlations among soil properties under different rice-upland crop systems and link selected soil properties to rice growth and yield, (ii) present appropriate values of soil parameters for sustainable rice productivity in heavy clay soil, (iii) evaluate the effect of rotating rice with upland crops on rice yield and economic benefit in a long-term experiment. A rice-upland crop rotational field experiment in the Vietnamese Mekong delta was conducted for 10 years using a randomized complete block design with four treatments and four replications. Treatments were: (i) rice-rice-rice (control - conventional system as farmers' practice), (ii) rice-maize-rice, (iii) rice-mung bean-rice, and (iv) rice-mung bean-maize. Soil and plant sampling were performed after harvest of the rice crop at the end of the final winter-spring cropping season (i.e. year 10). Results show differences in rice growth and yield, and economic benefit as an effect of the crop rotation system. These differences were linked with changes in bulk density, soil porosity, soil aggregate stability index, soil penetration resistance, soil macro-porosity, soil organic carbon, acid hydrolysable soil C and soil nutrient elements, especially at soil depth of 20-30 cm. This is evidenced by the strong correlation (P < 0.01) between rice plant parameters, rice yield and soil properties such as bulk density, porosity, penetration resistance, soil organic carbon and Chydrolysable. It turned out that good rice root growth and rice yield corresponded to bulk density values lower than 1.3 Mg m-3, soil porosity higher than 50%, penetration resistance below 1.0 MPa, and soil organic carbon above 25 g kg-1. The optimal

Gene flow from weedy red rice (Oryza sativa L.) to cultivated rice and fitness of hybrids.

PubMed

Shivrain, Vinod K; Burgos, Nilda R; Gealy, David R; Sales, Marites A; Smith, Kenneth L

2009-10-01

Gene transfer from weeds to crops could produce weedy individuals that might impact upon the evolutionary dynamics of weedy populations, the persistence of escaped genes in agroecosystems and approaches to weed management and containment of transgenic crops. The present aim was to quantify the gene flowrate from weedy red rice to cultivated rice, and evaluate the morphology, phenology and fecundity of resulting hybrids. Field experiments were conducted at Stuttgart and Rohwer, Arkansas, USA. Twelve red rice accessions and an imazethapyr-resistant rice (Imi-R; Clearfield) were used. Hybrids between Imi-R rice x red rice were 138-150 cm tall and flowered 1-5 days later than the rice parent, regardless of the red rice parent. Hybrids produced 20-50% more seed than the rice parent, but had equivalent seed production to the majority of red rice parents. Seeds of all hybrids were red, pubescent and dehisced at maturity. For the majority of hybrids, seed germination was higher than that of the red rice parent. The gene flowrate from red rice to rice was 0.01-0.2% and differed by red rice biotype. The hybrids had higher fecundity and potential competitive ability than the rice parent, and in some cases also the red rice parent. Red rice plants are vectors of gene flow back to cultivated rice and other weedy populations. The progeny of red rice hybrids from cultivated rice mother plants have higher chances of persistence than those from red rice mother plants. Gene flow mitigation strategies should consider this scenario. Copyright 2009 Society of Chemical Industry.

Identification of gonadal soma-derived factor involvement in Monopterus albus (protogynous rice field eel) sex change.

PubMed

Zhu, Yefei; Wang, Chunlei; Chen, Xiaowu; Guan, Guijun

2016-07-01

We studied molecular events and potential mechanisms underlying the process of female-to-male sex transformation in the rice field eel (Monopterus albus), a protogynous hermaphrodite fish in which the gonad is initially a female ovary and transforms into male testes. We cloned and identified a novel gonadal soma derived factor (GSDF), which encodes a member of the transforming growth factor-beta superfamily. gsdf expression was measured in gonads of female, intersex and male with reverse transcription-PCR and gsdf's role in sex transformation was studied with qPCR, histological analysis and dual-color in situ hybridization assays and compared to other sex-related genes. gsdf was correlated to Sertoli cell differentiation, indicating involvement in testicular differentiation and sex transformation from female to male in this species. A unique expression pattern reveals a potential role of gsdf essential for the sex transformation of rice field eels.

Pre-germinated brown rice could enhance maternal mental health and immunity during lactation.

PubMed

Sakamoto, Shigeko; Hayashi, Takashi; Hayashi, Keiko; Murai, Fumie; Hori, Miyo; Kimoto, Koichi; Murakami, Kazuo

2007-10-01

Rice is a dietary staple worldwide, especially pre-germinated brown rice has recently been widely served in Japan because of its abundant nutrition. Relationship between lactation and pre-germinated brown rice has attracted interest in terms of mental health and immunity. To demonstrate that Japanese foods are beneficial for psychosomatic health, the effects of pre-germinated brown rice on the mental status and immunological features during lactation were investigated. Forty-one breast-feeding mothers were recruited, and randomly divided into two groups. One group took pre-germinated brown rice and the other white rice (control) as their staple diet for 2 weeks. The Profile of Mood States (POMS) and salivary amylase activity as psychological indices and secretory IgA (s-IgA) and lactoferrin (LTF) in breast milk as immunological indices were determined before and after dietary intervention, and changes were investigated. In the psychological assessment, the scores of depression, anger-hostility, and fatigue were decreased on POMS analysis in the pre-germinated brown rice diet group, resulting in a significant decrease in total mood disturbance (TMD). The salivary amylase activity measurement suggested that resistance to stress was increased in the pre-germinated brown rice diet group. On the immunological assessment, the s-IgA level was significantly increased in the pre-germinated brown rice diet group. We have shown that pre-germinated brown rice may have beneficial effects on psychosomatic health.

[Interspecific relationship and Si, N nutrition of rice in rice-water spinach intercropping system.

PubMed

Ning, Chuan Chuan; Yang, Rong Shuang; Cai, Mao Xia; Wang, Jian Wu; Luo, Shi Ming; Cai, Kun Zheng

2017-02-01

Intercropping is a sound eco-agriculture model, but aquatic crops (e.g., rice) intercropping is seldom researched. In the present study, rice and water spinach were chosen as the research objects, a field trial was conducted to explore the yields, interspecific relationship and Si, N nutrition of rice under rice-water spinach intercropping for four seasons during two consecutive years (2014-2015). The experiment had five treatments: rice monoculture, water spinach monoculture, and rice-water spinach intercropping ratios of 2:2, 3:2, 4:2, respectively. The results showed that rice-water spinach intercropping significantly increased rice yield, and the increase rates of 2:2, 3:2 and 4:2 intercropping per unit area were 77.5%-120.6%, 64.9%-80.9%, 37.7%-56.0%, respectively. However, intercropping resulted in reduction of water spinach yield. Intercropping significantly increased total yield of rice and water spinach from land equivalent ratios (LER) analysis. The values of LER were more than 1.0, and the ratio of 3:2 intercropping had the best effect. As for the competitive index, rice was more competitive than water spinach in intercropping system, especially in early season. Compared with rice monoculture, rice-water spinach intercropping significantly increased the absorption of Si and N in rice leaves, and Si content of rice leaves during ripening stage, but didn't increase its N content and even slightly reduced it during ripening stage. Intercropping had no significant effect on available Si, ammonium N and nitrate N content in soil. Compared with rice monoculture and intercropping, water spinach monoculture had always the highest available Si, ammonium N and nitrate N contents in soil through the experiment period. The results suggested that rice-spinach intercropping could promote rice to absorb silicon and nitrogen and increase the competitive ability of rice.

The Chamber for Studying Rice Response to Elevated Nighttime Temperature in Field

PubMed Central

Chen, Song; Zheng, Xi; Wang, Dangying; Xu, Chunmei; Laza, Ma. Rebecca C.; Zhang, Xiufu

2013-01-01

An in situ temperature-controlled field chamber was developed for studying a large population of rice plant under different nighttime temperature treatments while maintaining conditions similar to those in the field during daytime. The system consists of a pipe hoop shed-type chamber with manually removable covers manipulated to provide a natural environment at daytime and a relatively stable and accurate temperature at night. Average air temperatures of 22.4 ± 0.3°C at setting of 22°C, 27.6 ± 0.4°C at 27°C, and 23.8 ± 0.7°C ambient conditions were maintained with the system. No significant horizontal and vertical differences in temperature were found and only slight changes in water temperatures were observed between the chambers and ambient conditions at 36 days after transplanting. A slight variation in CO2 concentration was observed at the end of the treatment during the day, but the 10-μmol CO2 mol−1 difference was too small to alter plant response. The present utilitarian system, which only utilizes an air conditioner/heater, is suitable for studying the effect of nighttime temperature on plant physiological responses with minimal perturbation of other environmental factors. At the same time, it will enable in situ screening of many rice genotypes. PMID:24089603

Mean field approximation for biased diffusion on Japanese inter-firm trading network.

PubMed

Watanabe, Hayafumi

2014-01-01

By analysing the financial data of firms across Japan, a nonlinear power law with an exponent of 1.3 was observed between the number of business partners (i.e. the degree of the inter-firm trading network) and sales. In a previous study using numerical simulations, we found that this scaling can be explained by both the money-transport model, where a firm (i.e. customer) distributes money to its out-edges (suppliers) in proportion to the in-degree of destinations, and by the correlations among the Japanese inter-firm trading network. However, in this previous study, we could not specifically identify what types of structure properties (or correlations) of the network determine the 1.3 exponent. In the present study, we more clearly elucidate the relationship between this nonlinear scaling and the network structure by applying mean-field approximation of the diffusion in a complex network to this money-transport model. Using theoretical analysis, we obtained the mean-field solution of the model and found that, in the case of the Japanese firms, the scaling exponent of 1.3 can be determined from the power law of the average degree of the nearest neighbours of the network with an exponent of -0.7.

[Prospective multi-institutional analysis according to the "Japanese guidelines for prevention of perioperative infections in urological field"].

PubMed

Wada, Koichiro; Uehara, Shinya; Kira, Shinichiro; Matsumoto, Masahiro; Sho, Takehiko; Kurimura, Yuichiro; Hashimoto, Jiro; Uehara, Teruhisa; Yamane, Takashi; Kanamaru, Sojun; Togo, Yoshikazu; Taoka, Rikiya; Takahashi, Akira; Yamada, Yusuke; Yokomizo, Akira; Yasuda, Mitsuru; Tanaka, Kazushi; Hamasuna, Ryoichi; Takahashi, Satoshi; Hayami, Hiroshi; Watanabe, Toyohiko; Monden, Koichi; Kiyota, Hiroshi; Deguchi, Takashi; Naito, Seiji; Tsukamoto, Taiji; Arakawa, Soichi; Fujisawa, Masato; Yamamoto, Shingo; Kumon, Hiromi; Matsumoto, Tetsuro

2013-05-01

The "Japanese guidelines for prevention of perioperative infections in urological field" was edited by the Japanese Urological Association in 2007. They are the first Japanese guidelines for antimicrobial prophylaxis specifically to prevent perioperative infections in the urological field. We report here the results of a multicenter prospective study conducted to examine the validity and usefulness of these guidelines. The subjects were 513 patients who had undergone urological surgeries between July and September 2008 at 10 nationwide university institutions in the Japanese Society of UTI Cooperative Study Group. These surgeries were transurethral resection of bladder (TURBT), transurethral resection of prostate (TURP), adrenalectomy, nephrectomy, nephroureterectomy, radical prostatectomy and total cystectomy. Analysis was performed on patient information, surgical procedures, types and durations of administration of prophylactic antibiotic agents, and the presence of surgical site infections (SSI) and remote infections (RI). Of 513 patients, 387 (75.4%) were administered prophylactic antibiotic agents according to the guidelines. In these patients, the incidences of SSI and RI were 5.9% and 4.1%, respectively. Multivariate analysis showed that significant factors for SSI were the surgical risk (according to the ASA physical status classification system), diabetes, and operation time, and that the only significant factor for RI was the operation time. More large-scale study and evidences are necessary in order to demonstrate the validity and usefulness of these guidelines.

Cytoplasmic-genetic male sterility gene provides direct evidence for some hybrid rice recently evolving into weedy rice

PubMed Central

Zhang, Jingxu; Lu, Zuomei; Dai, Weimin; Song, Xiaoling; Peng, Yufa; Valverde, Bernal E.; Qiang, Sheng

2015-01-01

Weedy rice infests paddy fields worldwide at an alarmingly increasing rate. There is substantial evidence indicating that many weedy rice forms originated from or are closely related to cultivated rice. There is suspicion that the outbreak of weedy rice in China may be related to widely grown hybrid rice due to its heterosis and the diversity of its progeny, but this notion remains unsupported by direct evidence. We screened weedy rice accessions by both genetic and molecular marker tests for the cytoplasmic male sterility (CMS) genes (Wild abortive, WA, and Boro type, BT) most widely used in the production of indica and japonica three-line hybrid rice as a diagnostic trait of direct parenthood. Sixteen weedy rice accessions of the 358 tested (4.5%) contained the CMS-WA gene; none contained the CMS-BT gene. These 16 accessions represent weedy rices recently evolved from maternal hybrid rice derivatives, given the primarily maternal inheritance of this trait. Our results provide key direct evidence that hybrid rice can be involved in the evolution of some weedy rice accessions, but is not a primary factor in the recent outbreak of weedy rice in China. PMID:26012494

Cytoplasmic-genetic male sterility gene provides direct evidence for some hybrid rice recently evolving into weedy rice.

PubMed

Zhang, Jingxu; Lu, Zuomei; Dai, Weimin; Song, Xiaoling; Peng, Yufa; Valverde, Bernal E; Qiang, Sheng

2015-05-27

Weedy rice infests paddy fields worldwide at an alarmingly increasing rate. There is substantial evidence indicating that many weedy rice forms originated from or are closely related to cultivated rice. There is suspicion that the outbreak of weedy rice in China may be related to widely grown hybrid rice due to its heterosis and the diversity of its progeny, but this notion remains unsupported by direct evidence. We screened weedy rice accessions by both genetic and molecular marker tests for the cytoplasmic male sterility (CMS) genes (Wild abortive, WA, and Boro type, BT) most widely used in the production of indica and japonica three-line hybrid rice as a diagnostic trait of direct parenthood. Sixteen weedy rice accessions of the 358 tested (4.5%) contained the CMS-WA gene; none contained the CMS-BT gene. These 16 accessions represent weedy rices recently evolved from maternal hybrid rice derivatives, given the primarily maternal inheritance of this trait. Our results provide key direct evidence that hybrid rice can be involved in the evolution of some weedy rice accessions, but is not a primary factor in the recent outbreak of weedy rice in China.

[Effects of rice cleaning and cooking process on the residues of flutolanil, fenobucarb, silafluofen and buprofezin in rice].

PubMed

Satoh, Motoaki; Sakaguchi, Masayuki; Kobata, Masakazu; Sakaguchi, Yoko; Tanizawa, Haruna; Miura, Yuri; Sasano, Ryoichi; Nakanishi, Yutaka

2003-02-01

We studied the effect of cleaning and cooking on the residues of flutolanil, fenobucarb, silafluofen and buprofezin in rice. The rice had been sprayed in a paddy field in Wakayama city, with 3 kinds of pesticide application protocols: spraying once at the usual concentration of pesticides, repeated spraying (3 times) with the usual concentration of pesticides and spraying once with 3 times the usual concentration of pesticides. The residue levels of pesticide decreased during the rice cleaning process. Silafluofen, which has a higher log Pow value, remained in the hull of the rice. Fenobucarb, which has a lower log Pow value, penetrated inside the rice. The residue concentration of pesticide in polished rice was higher than that in pre-washed rice processed ready for cooking. During the cooking procedure, the reduction of pesticides in polished rice was higher than that in brown rice.

Study of Culex tritaeniorhynchus and species composition of mosquitoes in a rice field in Greece.

PubMed

Lytra, Ioanna; Emmanouel, Nikolaos

2014-06-01

Mosquito species composition and seasonal abundance were studied in a rice field in western Greece over a three-year period (2009-2011). A total of 11,716 larvae and pupae of mosquitoes were recorded, representing seven species, namely Aedes caspius (Pallas), Anopheles hyrcanus (Pallas), Anopheles sacharovi Favre, Culex theileri Theobald, Culex tritaeniorhynchus Giles, Culex pipiens Linnaeus, Uranotaenia unguiculata Edwards and belonging to four genera. Cx. tritaeniorhynchus constituted the most abundant species. It is the second recorded occurrence of this species in Greece, but the first time that a high population of this mosquito species is recorded in the country. In all three years, the total population density of mosquitoes was found to be higher in early August. The number of immatures of all species was found higher in 2009 and 2010 than in 2011, as well was that of the Cx. tritaeniorhynchus adults derived from the rearing of the collected immatures. This regularity is probably due to the lack of water in the rice field in early August 2011. Cx. tritaeniorhynchus was found to be the most abundant species after the rearing of immatures representing 85.1%, 93.5% and 96.1% of the total number of the mosquito adults in 2009, 2010 and 2011, respectively. The rice culturing practices may have affected the seasonal occurrence of mosquito immatures in all of the study years. Copyright © 2014 Elsevier B.V. All rights reserved.

Effect of depth of flooding on the rice water weevil, Lissorhoptrus oryzophilus, and yield of rice.

PubMed

Tindall, Kelly V; Bernhardt, John L; Stout, Michael J; Beighley, Donn H

2013-01-01

The rice water weevil, Lissorhoptrus oryzophilus (Kuschel) (Coleoptera: Curculionidae), is a semi-aquatic pest of rice and is the most destructive insect pest of rice in the United States. Adults oviposit after floods are established, and greenhouse studies have shown that plants exposed to deep floods have more eggs oviposited in leaf sheaths than plants exposed to a shallow flood. Experiments were conducted in three mid-southern states in the USA to determine if the depth of flooding would impact numbers of L. oryzophilus on rice plants under field conditions. Rice was flooded at depths of approximately 5 or 10 cm in Arkansas in 2007 and 2008 and Louisiana in 2008, and at depths between 0-20 cm in Missouri in 2008. Plants were sampled three and four weeks after floods were established in all locations, and also two weeks after flood in Missouri. On all sampling dates in four experiments over two years and at three field sites, fewer L. oryzophilus larvae were collected from rice in shallow-flooded plots than from deep-flooded plots. The number of L. oryzophilus was reduced by as much as 27% in shallow-flooded plots. However, the reduction in insect numbers did not translate to a significant increase in rice yield. We discuss how shallow floods could be used as a component of an integrated pest management program for L. oryzophilus.

Effect of Depth of Flooding on the Rice Water Weevil, Lissorhoptrus oryzophilus, and Yield of Rice

PubMed Central

Tindall, Kelly V.; Bernhardt, John L.; Stout, Michael J.; Beighley, Donn H.

2013-01-01

The rice water weevil, Lissorhoptrus oryzophilus (Kuschel) (Coleoptera: Curculionidae), is a semi-aquatic pest of rice and is the most destructive insect pest of rice in the United States. Adults oviposit after floods are established, and greenhouse studies have shown that plants exposed to deep floods have more eggs oviposited in leaf sheaths than plants exposed to a shallow flood. Experiments were conducted in three mid-southern states in the USA to determine if the depth of flooding would impact numbers of L. oryzophilus on rice plants under field conditions. Rice was flooded at depths of approximately 5 or 10 cm in Arkansas in 2007 and 2008 and Louisiana in 2008, and at depths between 0–20 cm in Missouri in 2008. Plants were sampled three and four weeks after floods were established in all locations, and also two weeks after flood in Missouri. On all sampling dates in four experiments over two years and at three field sites, fewer L. oryzophilus larvae were collected from rice in shallow-flooded plots than from deep-flooded plots. The number of L. oryzophilus was reduced by as much as 27% in shallow-flooded plots. However, the reduction in insect numbers did not translate to a significant increase in rice yield. We discuss how shallow floods could be used as a component of an integrated pest management program for L. oryzophilus. PMID:23906324

Long-term cultivation of Bt rice expressing the Cry1Ab/1Ac gene reduced phytoparasitic nematode abundance but did not affect other nematode parameters in paddy fields.

PubMed

Chen, Qunying; Yang, Bing; Liu, Xianghui; Chen, Fajun; Ge, Feng

2017-12-31

The uncertainty of ecological risks and the effects of growing transgenic Bt rice on the environment hamper its commercial production. Here, soil nematode communities were used as an indicator of soil health and soil food web structure to evaluate the potential effects of growing Bt rice without chemical insecticides for 3years in the paddy field. The nematodes and soil physicochemical properties of Bt rice fields were compared to the near-isogenic control, non-Bt rice fields. A total of 108,363 specimens belonging to 28 different genera were enumerated. The Hirschmanniella, Tobrilus, Dorylaimus and Filenchus were dominant genera. A three-year paddy rice cultivation of Bt rice (Huahui 1) negatively affected the abundance of phytoparasitic nematodes but did not affect the total number of nematodes, the abundance and relative abundance of free-living nematodes, genera richness, diversity indices, soil food web conditions, or community compositions. However, apparent seasonal and inter-annual changes in these variables were observed, indicating that the impact of environmental factors was more stronger than that of the Bt toxin. In conclusion, the potential ecological risks of Bt rice on soil health and sustainability warrant further research to disentangle the impacts from various confounding environmental factors. Copyright © 2017 Elsevier B.V. All rights reserved.

Homologous expression of cytosolic dehydroascorbate reductase increases grain yield and biomass under paddy field conditions in transgenic rice (Oryza sativa L. japonica).

PubMed

Kim, Young-Saeng; Kim, Il-Sup; Bae, Mi-Jung; Choe, Yong-Hoe; Kim, Yul-Ho; Park, Hyang-Mi; Kang, Hong-Gyu; Yoon, Ho-Sung

2013-06-01

Dehydroascorbate reductase (DHAR, EC 1.8.5.1) maintains redox pools of ascorbate (AsA) by recycling oxidized AsA to reduced AsA. To investigate whether DHAR affects rice yield under normal environmental conditions, cDNA-encoding DHAR (OsDHAR1) was isolated from rice and used to develop OsDHAR1-overexpressing transgenic rice plants, under the regulation of a maize ubiquitin promoter. Incorporation and expression of the transgene in transgenic rice plants was confirmed by genomic polymerase chain reaction (PCR), semi-quantitative reverse transcription PCR (RT-PCR), western blot, and enzyme activity. The expression levels were at least twofold higher in transgenic (TG) rice plants than in control wild-type (WT) rice plants. In addition, OsDHAR1-overexpression in seven-independent homologous transgenic plants, as compared to WT plants, increased photosynthetic capacity and antioxidant enzyme activities under paddy field conditions, which led to an improved AsA pool and redox homeostasis. Furthermore, OsDHAR1 overexpression significantly improved grain yield and biomass due to the increase of culm and root weights and to enhance panicle and spikelet numbers in the same seven independent TG rice plants during the farming season (2010 and 2011) in South Korea. The OsDHAR protein contained the redox-active site (Cys20), as well as the conserved GSH-binding region, GSH-binding motif, glutathione-S-transferase (GST) N-terminal domain, C-terminal domain interface, and GST C-terminal domain. Therefore, our results indicate that OsDHAR1 overexpression, capable of functioning in AsA recycling, and protein folding increases environmental adaptation to paddy field conditions by the improving AsA pool and redox homeostasis, which enhances rice grain yield and biomass.

Sequential Classifier Training for Rice Mapping with Multitemporal Remote Sensing Imagery

NASA Astrophysics Data System (ADS)

Guo, Y.; Jia, X.; Paull, D.

2017-10-01

Most traditional methods for rice mapping with remote sensing data are effective when they are applied to the initial growing stage of rice, as the practice of flooding during this period makes the spectral characteristics of rice fields more distinguishable. In this study, we propose a sequential classifier training approach for rice mapping that can be used over the whole growing period of rice for monitoring various growth stages. Rice fields are firstly identified during the initial flooding period. The identified rice fields are used as training data to train a classifier that separates rice and non-rice pixels. The classifier is then used as a priori knowledge to assist the training of classifiers for later rice growing stages. This approach can be applied progressively to sequential image data, with only a small amount of training samples being required from each image. In order to demonstrate the effectiveness of the proposed approach, experiments were conducted at one of the major rice-growing areas in Australia. The proposed approach was applied to a set of multitemporal remote sensing images acquired by the Sentinel-2A satellite. Experimental results show that, compared with traditional spectral-indexbased algorithms, the proposed method is able to achieve more stable and consistent rice mapping accuracies and it reaches higher than 80% during the whole rice growing period.

Elucidation of molecular dynamics of invasive species of rice

USDA-ARS?s Scientific Manuscript database

Cultivated rice fields are aggressively invaded by weedy rice in the U.S. and worldwide. Weedy rice results in loss of yield and seed contamination. The molecular dynamics of the evolutionary adaptive traits of weedy rice are not fully understood. To understand the molecular basis and identify the i...

Drainage and tillage practices in the winter fallow season mitigate CH4 and N2O emissions from a double-rice field in China

NASA Astrophysics Data System (ADS)

Zhang, Guangbin; Yu, Haiyang; Fan, Xianfang; Yang, Yuting; Ma, Jing; Xu, Hua

2016-09-01

Traditional land management (no tillage, no drainage, NTND) during the winter fallow season results in substantial CH4 and N2O emissions from double-rice fields in China. A field experiment was conducted to investigate the effects of drainage and tillage during the winter fallow season on CH4 and N2O emissions and to develop mitigation options. The experiment had four treatments: NTND, NTD (drainage but no tillage), TND (tillage but no drainage), and TD (both drainage and tillage). The study was conducted from 2010 to 2014 in a Chinese double-rice field. During winter, total precipitation and mean daily temperature significantly affected CH4 emission. Compared to NTND, drainage and tillage decreased annual CH4 emissions in early- and late-rice seasons by 54 and 33 kg CH4 ha-1 yr-1, respectively. Drainage and tillage increased N2O emissions in the winter fallow season but reduced it in early- and late-rice seasons, resulting in no annual change in N2O emission. Global warming potentials of CH4 and N2O emissions were decreased by 1.49 and 0.92 t CO2 eq. ha-1 yr-1, respectively, and were reduced more by combining drainage with tillage, providing a mitigation potential of 1.96 t CO2 eq. ha-1 yr-1. A low total C content and high C / N ratio in rice residues showed that tillage in the winter fallow season reduced CH4 and N2O emissions in both early- and late-rice seasons. Drainage and tillage significantly decreased the abundance of methanogens in paddy soil, and this may explain the decrease of CH4 emissions. Greenhouse gas intensity was significantly decreased by drainage and tillage separately, and the reduction was greater by combining drainage with tillage, resulting in a reduction of 0.17 t CO2 eq. t-1. The results indicate that drainage combined with tillage during the winter fallow season is an effective strategy for mitigating greenhouse gas releases from double-rice fields.

The impact of elevated CO2 and temperature on grain quality of rice grown under open-air field conditions.

PubMed

Jing, Liquan; Wang, Juan; Shen, Shibo; Wang, Yunxia; Zhu, Jianguo; Wang, Yulong; Yang, Lianxin

2016-08-01

Rising atmospheric CO2 is accompanied by global warming. However, interactive effects of elevated CO2 and temperature have not been well studied on grain quality of rice. A japonica cultivar was grown in the field using a free-air CO2 enrichment facility in combination with a canopy air temperature increase system in 2014. The gas fumigation (200 µmol mol(-1) above ambient CO2 ) and temperature increase (1 °C above ambient air temperature) were performed from tillering until maturity. Compared with the control (ambient CO2 and air temperature), elevated CO2 increased grain length and width as well as grain chalkiness but decreased protein concentrations. In contrast, the increase in canopy air temperature had less effect on these parameters except for grain chalkiness. The starch pasting properties of rice flour and taste analysis of cooked rice indicated that the palatability of rice was improved by CO2 and/or temperature elevation, with the combination of the two treatments showing the most significant changes compared with ambient rice. It is concluded that projected CO2 in 2050 may have larger effects on rice grain quality than the projected temperature increase. Although deterioration in milling suitability, grain appearance and nutritional quality can be expected, the taste of cooked rice might be better in the future environment. © 2015 Society of Chemical Industry. © 2015 Society of Chemical Industry.

Rice methylmercury exposure and mitigation: a comprehensive review.

PubMed

Rothenberg, Sarah E; Windham-Myers, Lisamarie; Creswell, Joel E

2014-08-01

Rice cultivation practices from field preparation to post-harvest transform rice paddies into hot spots for microbial mercury methylation, converting less-toxic inorganic mercury to more-toxic methylmercury, which is likely translocated to rice grain. This review includes 51 studies reporting rice total mercury and/or methylmercury concentrations, based on rice (Orzya sativa) cultivated or purchased in 15 countries. Not surprisingly, both rice total mercury and methylmercury levels were significantly higher in polluted sites compared to non-polluted sites (Wilcoxon rank sum, p<0.001). However, rice percent methylmercury (of total mercury) did not differ statistically between polluted and non-polluted sites (Wilcoxon rank sum, p=0.35), suggesting comparable mercury methylation rates in paddy soil across these sites and/or similar accumulation of mercury species for these rice cultivars. Studies characterizing the effects of rice cultivation under more aerobic conditions were reviewed to determine the mitigation potential of this practice. Rice management practices utilizing alternating wetting and drying (instead of continuous flooding) caused soil methylmercury levels to spike, resulting in a strong methylmercury pulse after fields were dried and reflooded; however, it is uncertain whether this led to increased translocation of methylmercury from paddy soil to rice grain. Due to the potential health risks, it is advisable to investigate this issue further, and to develop separate water management strategies for mercury polluted and non-polluted sites, in order to minimize methylmercury exposure through rice ingestion. Copyright © 2014 Elsevier Inc. All rights reserved.

Rice methylmercury exposure and mitigation: a comprehensive review

USGS Publications Warehouse

Rothenberg, Sarah E.; Windham-Myers, Lisamarie; Creswell, Joel E.

2014-01-01

Rice cultivation practices from field preparation to post-harvest transform rice paddies into hot spots for microbial mercury methylation, converting less-toxic inorganic mercury to more-toxic methylmercury, which is likely translocated to rice grain. This review includes 51 studies reporting rice total mercury and/or methylmercury concentrations, based on rice (Orzya sativa) cultivated or purchased in 15 countries. Not surprisingly, both rice total mercury and methylmercury levels were significantly higher in polluted sites compared to non-polluted sites (Wilcoxon rank sum, p<0.001). However, rice percent methylmercury (of total mercury) did not differ statistically between polluted and non-polluted sites (Wilcoxon rank sum, p=0.35), suggesting comparable mercury methylation rates in paddy soil across these sites and/or similar accumulation of mercury species for these rice cultivars. Studies characterizing the effects of rice cultivation under more aerobic conditions were reviewed to determine the mitigation potential of this practice. Rice management practices utilizing alternating wetting and drying (instead of continuous flooding) caused soil methylmercury levels to spike, resulting in a strong methylmercury pulse after fields were dried and reflooded; however, it is uncertain whether this led to increased translocation of methylmercury from paddy soil to rice grain. Due to the potential health risks, it is advisable to investigate this issue further, and to develop separate water management strategies for mercury polluted and non-polluted sites, in order to minimize methylmercury exposure through rice ingestion.

Rice Methylmercury Exposure and Mitigation: A Comprehensive Review

PubMed Central

Rothenberg, Sarah E.; Windham-Myers, Lisamarie; Creswell, Joel E.

2014-01-01

Rice cultivation practices from field preparation to post-harvest transform rice paddies into hot spots for microbial mercury methylation, converting less-toxic inorganic mercury to more-toxic methylmercury, which is likely translocated to rice grain. This review includes 51 studies reporting rice total mercury and/or methylmercury concentrations, based on rice cultivated or purchased in 15 countries. Not surprisingly, both rice total mercury and methylmercury levels were significantly higher in polluted sites compared to non-polluted sites (Wilcoxon rank sum, p<0.001). However, rice percent methylmercury (of total mercury) did not differ statistically between polluted and non-polluted sites (Wilcoxon rank sum, p=0.35), suggesting comparable mercury methylation rates in paddy soil across these sites and/or similar accumulation of mercury species for these rice cultivars. Studies characterizing the effect of rice cultivation under more aerobic conditions were reviewed to determine the mitigation potential of this practice. Rice management practices utilizing alternating wetting and drying (instead of continuous flooding) caused soil methylmercury levels to spike, resulting in a strong methylmercury pulse after fields were dried and reflooded; however, it is uncertain whether this led to increased translocation of methylmercury from paddy soil to rice grain. Due to the potential health risks, it is advisable to investigate this issue further, and to develop separate water management strategies for mercury polluted and non-polluted sites, which minimize methylmercury exposure through rice ingestion. PMID:24972509

Overexpression of an Arabidopsis thaliana galactinol synthase gene improves drought tolerance in transgenic rice and increased grain yield in the field.

PubMed

Selvaraj, Michael Gomez; Ishizaki, Takuma; Valencia, Milton; Ogawa, Satoshi; Dedicova, Beata; Ogata, Takuya; Yoshiwara, Kyouko; Maruyama, Kyonoshin; Kusano, Miyako; Saito, Kazuki; Takahashi, Fuminori; Shinozaki, Kazuo; Nakashima, Kazuo; Ishitani, Manabu

2017-11-01

Drought stress has often caused significant decreases in crop production which could be associated with global warming. Enhancing drought tolerance without a grain yield penalty has been a great challenge in crop improvement. Here, we report the Arabidopsis thaliana galactinol synthase 2 gene (AtGolS2) was able to confer drought tolerance and increase grain yield in two different rice (Oryza sativa) genotypes under dry field conditions. The developed transgenic lines expressing AtGolS2 under the control of the constitutive maize ubiquitin promoter (Ubi:AtGolS2) also had higher levels of galactinol than the non-transgenic control. The increased grain yield of the transgenic rice under drought conditions was related to a higher number of panicles, grain fertility and biomass. Extensive confined field trials using Ubi:AtGolS2 transgenic lines in Curinga, tropical japonica and NERICA4, interspecific hybrid across two different seasons and environments revealed the verified lines have the proven field drought tolerance of the Ubi:AtGolS2 transgenic rice. The amended drought tolerance was associated with higher relative water content of leaves, higher photosynthesis activity, lesser reduction in plant growth and faster recovering ability. Collectively, our results provide strong evidence that AtGolS2 is a useful biotechnological tool to reduce grain yield losses in rice beyond genetic differences under field drought stress. © 2017 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.

Rice crop growth monitoring using ENVISAT-1/ASAR AP mode

NASA Astrophysics Data System (ADS)

Konishi, Tomohisa; Suga, Yuzo; Omatu, Shigeru; Takeuchi, Shoji; Asonuma, Kazuyoshi

2007-10-01

Hiroshima Institute of Technology (HIT) is operating the direct down-links of microwave and optical earth observation satellite data in Japan. This study focuses on the validation for rice crop monitoring using microwave remotely sensed image data acquired by ENIVISAT-1 referring to ground truth data such as height of rice crop, vegetation cover rate and leaf area index in the test sites of Hiroshima district, the western part of Japan. ENVISAT-1/ASAR data has the capabilities for the monitoring of the rice crop growing cycle by using alternating cross polarization mode images. However, ASAR data is influenced by several parameters such as land cover structure, direction and alignment of rice crop fields in the test sites. In this study, the validation was carried out to be combined with microwave image data and ground truth data regarding rice crop fields to investigate the above parameters. Multi-temporal, multi-direction (descending and ascending) and multi-angle ASAR alternating cross polarization mode images were used to investigate during the rice crop growing cycle. On the other hand, LANDSAT-7/ETM+ data were used to detect land cover structure, direction and alignment of rice crop fields corresponding to the backscatter of ASAR. Finally, the extraction of rice planted area was attempted by using multi-temporal ASAR AP mode data such as VV/VH and HH/HV. As the result of this study, it is clear that the estimated rice planted area coincides with the existing statistical data for area of the rice crop field. In addition, HH/HV is more effective than VV/VH in the rice planted area extraction.

Understanding of evolutionary genomics of invasive species of rice

USDA-ARS?s Scientific Manuscript database

Red rice is an aggressive, weedy form of cultivated rice (Oryza sativa) that infests crop fields and is a primary factor limiting rice productivity in the U.S. and worldwide. As the weedy relative of a genomic model species, red rice is a model for understanding the genetic and evolutionary mechani...

Evaluating health of paddy rice field ecosystem with remote sensing and GIS in Lower Yangtze River Plain, China

NASA Astrophysics Data System (ADS)

Li, Jingjing; Qin, Zhihao; Li, Wenjuan; Lin, Lu

2008-10-01

A paddy rice ecosystem is a farming system composed of paddy, animals, microbes and other environmental factors in specific time and space, with particular temporal and spatial dynamics. Since paddy rice is a main grain crop to feed above half of population in China, the performance of paddy rice ecosystem is highly concerned to yield level of paddy and food supply safety in China. Therefore, monitoring the performance of paddy rice ecosystem is very important to obtain the required information for evaluation of ecosystem health. In the study we intend to develop an approach to monitor the ecosystem performance spatially and dynamically in a regional scale using MODIS remote sensing data and GIS spatial mapping. On the basis of key factors governing the paddy rice ecosystem, we accordingly develop the following three indicators for the evaluation: Crop growing index (CGI), environmental Index (EI), and pests-diseases index (PDI). Then, we integrated the three indicators into a model with different weight coefficients to calculate Comprehensive ecosystem health index (CEHI) to evaluate the performance and functioning of paddy rice ecosystem in a regional scale. CGI indicates the health status of paddy rice calculated from the normalizing enhanced vegetation Index (EVI) retrieved from MODIS data. EI is estimated from temperature Index (TI) and precipitation Index (PI) indicating heat and water stress on the rice field. PDI reflects the damage brought by pests and diseases, which can be estimated using the information obtained from governmental websites. Applying the approach to Lower Yangtze River Plain, we monitor and evaluate the performance of paddy rice ecosystem in various stages of rice growing period in 2006. The results indicated that the performance of the ecosystem was generally very encouraging. During booting stage and heading and blooming stage, the health level was the highest in Anhui province, which is the main paddy rice producer in the region

Winter fluxes from Eastern Arkansas Rice-Waterfowl Habitats

NASA Astrophysics Data System (ADS)

Reba, M. L.; Fong, B.; Runkle, B.; Suvocarev, K.; Adviento-Borbe, A.

2016-12-01

Seasonal flooding of rice fields in the mid-South for migratory birds during the winter months (December- January) has occurred for years. This practice can impact total annual greenhouse gas (GHG) emissions and fluxes during the production season (June-August). Over 75% of US rice production occurs in the mid-South, but limited research has analyzed the winter fluxes of methane and carbon dioxide in this rice-waterfowl habitat. Usually rice fields are flooded from June to August for the production season, and again December to January for migratory birds. In addition to hunting revenue, added benefits of winter flooding include weed control and prevention of soil oxidation and subsidence. Eddy covariance systems measuring carbon dioxide, water vapor and methane fluxes were installed at two sites in northeastern and east central Arkansas. Each site had two systems on neighboring fields with one flooded and the other not flooded. Seasonal variability in fluxes were compared and contrasted during the 2015-2016 winter. Both carbon dioxide and methane fluxes were positively related to temperatures. These findings will improve the understanding of seasonal greenhouse gas emissions at a field scale under typical mid-South rice production practices.

Genome-Wide Association of Rice Blast Disease Resistance and Yield-Related Components of Rice.

PubMed

Wang, Xueyan; Jia, Melissa H; Ghai, Pooja; Lee, Fleet N; Jia, Yulin

2015-12-01

Robust disease resistance may require an expenditure of energy that may limit crop yield potential. In the present study, a subset of a United States Department of Agriculture rice core collection consisting of 151 accessions was selected using a major blast resistance (R) gene, Pi-ta, marker and was genotyped with 156 simple sequence repeat (SSR) markers. Disease reactions to Magnaporthe oryzae, the causal agent of rice blast disease, were evaluated under greenhouse and field conditions, and heading date, plant height, paddy and brown seed weight in two field environments were analyzed, using an association mapping approach. A total of 21 SSR markers distributed among rice chromosomes 2 to 12 were associated with blast resistance, and 16 SSR markers were associated with seed weight, heading date, and plant height. Most noticeably, shorter plants were significantly correlated with resistance to blast, rice genomes with Pi-ta were associated with lighter seed weights, and the susceptible alleles of RM171 and RM6544 were associated with heavier seed weight. These findings unraveled a complex relationship between disease resistance and yield-related components.

Genetic diversity of weedy red rice (Oryza sativa) in Arkansas, USA

USDA-ARS?s Scientific Manuscript database

Weedy red rice (Oryza sativa L.) is a problematic weed in rice. About 50% of US rice is produced in Arkansas and 60% of these fields have some red rice infestation. Red rice populations are morphologically and phenologically diverse. We hypothesize that red rice in Arkansas has high genetic diversit...

Insights into molecular mechanism of blast resistance in weedy rice

USDA-ARS?s Scientific Manuscript database

Weedy rice is a serious pest in direct-seeded rice fields in the U.S. and worldwide. Under suitable conditions, weedy rice can reduce crop yields up to 70%. However, weedy rice may carry novel disease resistance genes. Rice blast disease caused by the fungus Magnaporthe oryzae is a major disease wo...

[Effect of transgenic insect-resistant rice on biodiversity].

PubMed

Zhang, Lei; Zhu, Zhen

2011-05-01

Rice is the most important food crops in maintaining food security in China. The loss of China's annual rice production caused by pests is over ten million tons. Present studies showed that the transgenic insect-resistant rice can substantially reduce the application amount of chemical pesticides. In the case of no pesticide use, the pest density in transgenic rice field is significantly lower than that in non-transgenic field, and the neutral insects and natural enemies of pests increased significantly, indicating that the ecological environment and biodiversity toward the positive direction. The gene flow frequency from transgenic rice is dramatically reduced with the distance increases, reaching less than 0.01% at the distance of 6.2 m. Application of transgenic insect-resistant rice in China has an important significance for ensuring food security, maintaining sustainable agricultural development, and protecting the ecological environment and biodiversity. This review summarized the research progress in transgenic insect-resistant rice and its effect on biodiversity. The research directions and development trends of crop pest controlling in future are discussed. These help to promote better use of transgenic insect-resistant rice.

Azodrin® poisoning of waterfowl in rice fields in Louisiana

USGS Publications Warehouse

White, D.H.; Mitchell, C.A.; Kolbe, E.J.; Ferguson, W.H.

1983-01-01

During the period 2-4 April 1981 about 100 birds, mostly ducks and geese, were found dead and dying in a rice field near Sweet Lake, Calcasieu Parish, Louisiana. Fresh specimens were collected to determine the cause of mortality. Birds were placed individually in polyethylene freezer bags, tagged, and frozen soon after collection. Four snow geese (Chen caerulescens), two blue-winged teal (Anas discors), one green-winged teal (Anas crecca), and one mottled duck (Anas fulvigula) were shipped to the National Wildlife Health Laboratory (NWHL), Madison, Wisconsin, for necropsy and pathological examination. Ten snow geese, 10 blue-winged teal, three green-winged teal, three great-tailed grackles (Quiscalus mexicanus), and eight red-winged blackbirds (Agelaius phoeniceus) were transported to the Gulf Coast Field Station, Victoria, Texas, for brain acetylcholinesterase (AChE) activity determinations and preparation for chemical residue analysis. Additionally, apparently healthy specimens of the affected species were collected near Lake Charles, Louisiana, and Victoria, Texas, to serve as controls in the analyses.

Analysis of the eukaryotic community and metabolites found in clay wall material used in the construction of traditional Japanese buildings.

PubMed

Kitajima, Sakihito; Kamei, Kaeko; Nishitani, Maiko; Sato, Hiroyuki

2010-01-01

Clay wall (tsuchikabe in Japanese) material for Japanese traditional buildings is manufactured by fermenting a mixture of clay, sand, and rice straw. The aim of this study was to understand the fermentation process in order to gain insight into the ways waste biomass can be used to produce useful materials. In this study, in addition to Clostridium, we suggested that the family Nectriaceae and the Scutellinia sp. of fungi were important in degrading cell wall materials of rice straw, such as cellulose and/or lignin. The microorganisms in the clay wall material produced sulfur-containing inorganic compounds that may sulfurate minerals in clay particles, and polysaccharides that give viscosity to clay wall material, thus increasing workability for plastering, and possibly giving water-resistance to the dried clay wall.

Temporal changes of radiocesium in irrigated paddy fields and its accumulation in rice plants in Fukushima.

PubMed

Yang, Baolu; Onda, Yuichi; Wakiyama, Yoshifumi; Yoshimura, Kazuya; Sekimoto, Hitoshi; Ha, Yiming

2016-01-01

About half of the total paddy field area, which is the dominant agricultural land in Fukushima Prefecture, was contaminated by radiocesium released by the Fukushima Daiichi Nuclear Power Plant accident. In this study, we investigated the temporal changes of radiocesium in soil, irrigation water, and rice plant in two adjacent rice paddies, with and without surface-soil-removal, in Fukushima Prefecture for over three years (2012-2014) after the nuclear accident. Our results showed that radiocesium migrated into 24-28 cm soil layers and that the activity concentration of radiocesium in paddy soils showed a significant reduction in 2014. The newly added radiocesium to paddies through irrigation water contributed only a maximum value of 0.15% and 0.75% of the total amount present in control and decontaminated paddies, respectively, throughout the study period. The radiocesium activity concentration in suspended sediment in irrigation water exponentially decreased, and the effective half-lives (Teff) for (137)Cs and (134)Cs were 1.3 and 0.9 years, respectively. Additionally, the average suspended sediment concentration in irrigation water increased between 2012 and 2014, suggesting that enhanced soil erosion had occurred in the surrounding environment. Radiocesium accumulation in rice plant also decreased with time in both paddies. However, the concentration ratio of radiocesium for rice plant in the decontaminated paddy increased compared with control paddy, despite approximately 96% of fallout radiocesium removed in paddy soil. Further analysis is required to clarify the reasons of high concentration ratio of radiocesium for rice plant in the decontaminated paddy. Copyright © 2015 Elsevier Ltd. All rights reserved.

An Approach to Assessing Flood Risk in Low-lying Paddy Areas of Japan considering Economic Damage on Rice

NASA Astrophysics Data System (ADS)

Minakawa, H.; Masumoto, T.

2013-12-01

Hiroki Minakawa, Takao Masumoto National Institute for Rural Engineering (NIRE), NARO, Japan Flooding is one type of nature disaster, and is caused by heavy rainfall events. In the future, the risk of flooding is predicted to increase due to global climate change. Immediate measures such as strengthening drainage capacity are needed to minimize the damage caused by more frequent flooding, so a quantitative evaluation method of flood risks is needed to discuss countermeasure against these problems. At the same time, rice is an important crop for food production in Japan. However, paddy fields are often damaged by flooding because they are principally spread in lower part of the basin. Therefore, it is also important to assess the damages to paddy fields. This study discusses a method for evaluating a relationship between the risk of flood damage and the scale of heavy rainfall. We also developed a method of estimating the economic effect of a reduction in rice yield by flooding. First, we developed a drainage analysis model that incorporates kinematic and diffusive runoff models for calculating water level in channels and paddies. Next, heavy rainfall data for drainage analyses were generated by using a diurnal rainfall pattern generator. The generator can create hourly data of heavy rainfall, and internal pattern of them is different each. These data were input to the drainage model to estimate flood risk. Simultaneously, we tried to clarify economic losses of a rice yields caused by flooding. Here, the reduction scale in rice yield which shows relations between flooding situation (e.g. water level, duration of submersion etc.) and damage of rice is available to calculate reduction of rice yield. In this study, we created new reduction scales through a pseudo-flooding experiment under real inundation conditions. The methodology of the experiment was as follow: We chose the popular Japanese rice cultivar Koshihikari for this experiment. An experimental arena was

Association of Commercial Rice Varieties with Weedy Rice Accessions (Oryza sativa complex) in Pulau Pinang's Rice Granary Area.

PubMed

Hussain, Zainudin; Man, Azmi; Othman, Ahmad Sofiman

2011-12-01

Weedy rice (WR) is the most significant weed in direct-seeded fields. It has morphological characteristics similar to those of cultivated rice varieties. WR is more difficult to control than other weeds. We collected WR accessions from four sites within the Pulau Pinang rice growing areas. Thirty six different accessions were collected from each site: B, the northern site; P, the central site; A, the southern site; and N, the southwestern site. Wild rice (Oryza rufipogon), which grows in the sampled areas, was also collected together with four varieties (MR84, MR185, MR211 and MR219) that have been widely planted in these areas for a long period of time. The objective of this study was to compare the morphological characteristics of the WR accessions and cultivated rice. Twenty characteristics were observed for the comparison of WR accessions and rice cultivars. Morpho-matrix analyses allowed the specimens to be grouped to two main groups (A and B), based on a 95% dissimilarity matrix. Group A was subdivided into 7 subgroups consisting of a few WR accessions, wild rice and MR211 (control), and group B was subdivided to 10 subgroups consisting of other WR accessions and the 3 other control varieties. Dendrogram analysis indicated that the morphological traits used in this study were able to differentiate among the WR accessions and the cultivars, except for rice cultivar MR211 and WRA8, which grouped together in subgroup A2. STRUCTURE program analysis indicated that all individuals were distinguishable and were divided into 18 clusters. These results suggest that some genes of the WR accessions have been influenced by commercial varieties. The information gained from this study will be useful to develop rice weed management protocols and good agricultural practices to control WR in the future.

Effects of straw incorporation along with microbial inoculant on methane and nitrous oxide emissions from rice fields.

PubMed

Liu, Gang; Yu, Haiyang; Ma, Jing; Xu, Hua; Wu, Qinyan; Yang, Jinghui; Zhuang, Yiqing

2015-06-15

Incorporation of straw together with microbial inoculant (a microorganism agent, accelerating straw decomposition) is being increasingly adopted in rice cultivation, thus its effect on greenhouse gas (GHG) emissions merits serious attention. A 3-year field experiment was conducted from 2010 to 2012 to investigate combined effect of straw and microbial inoculant on methane (CH4) and nitrous oxide (N2O) emissions, global warming potential (GWP) and greenhouse gas intensity (GHGI) in a rice field in Jurong, Jiangsu Province, China. The experiment was designed to have treatment NPK (N, P and K fertilizers only), treatment NPKS (NPK plus wheat straw), treatment NPKSR (NPKS plus Ruilaite microbial inoculant) and treatment NPKSJ (NPKS plus Jinkuizi microbial inoculant). Results show that compared to NPK, NPKS increased seasonal CH4 emission by 280-1370%, while decreasing N2O emission by 7-13%. When compared with NPKS, NPKSR and NPKSJ increased seasonal CH4 emission by 7-13% and 6-12%, respectively, whereas reduced N2O emission by 10-27% and 9-24%, respectively. The higher CH4 emission could be attributed to the higher soil CH4 production potential triggered by the combined application of straw and microbial inoculant, and the lower N2O emission to the decreased inorganic N content. As a whole, the benefit of lower N2O emission was completely offset by increased CH4 emission, resulting in a higher GWP for NPKSR (5-12%) and NPKSJ (5-11%) relative to NPKS. Due to NPKSR and NPKSJ increased rice grain yield by 3-6% and 2-4% compared to NPKS, the GHGI values for NPKS, NPKSR and NPKSJ were comparable. These findings suggest that incorporating straw together with microbial inoculant would not influence the radiative forcing of rice production in the terms of per unit of rice grain yield relative to the incorporation of straw alone. Copyright © 2015 Elsevier B.V. All rights reserved.

Screening and selection of most potent diazotrophic cyanobacterial isolate exhibiting natural tolerance to rice field herbicides for exploitation as biofertilizer.

PubMed

Singh, Surendra; Datta, Pallavi

2006-01-01

Periodic applications of heavy dosages of herbicides in modern rice-agriculture are a necessary evil for obtaining high crop productivity. Such herbicides are not only detrimental to weeds but biofertilizer strains of diazotrophic cyanobacteria also. It is therefore, essential to screen and select such biofertilizer strains of diazotrophic cyanobacteria exhibiting natural tolerance to common rice-field herbicides that can be further improved by mutational techniques to make biofertilizer technology a viable one. Therefore, efforts have been made to screen five dominant diazotrophic cyanobacterial forms e.g. filamentous heterocystous Nostoc punctiforme , Nostoc calcicola , Anabaena variabilis and unicellular Gloeocapsa sp. and Aphanocapsa sp. along with standard laboratory strain Nostoc muscorum ISU against increasing concentrations (0-100 mg l(-1) of four commercial grade common rice-field herbicides i.e. Arozin, Butachlor, Alachlor and 2,4-D under diazotrophic growth conditions. The lethal and IGC(50) concentrations for all four herbicides tested were found highest for A. variabilis as compared to other test cyanobacteria. The lowest reduction in chlorophyll a content, photosynthetic oxygen evolution, and N(2)-fixation was found in A. variabilis as compared to other rice field isolates and standard laboratory strain N. muscorum ISU. On the basis of prolong survival potential and lowest reductions in vital metabolic activities tested at IGC(50) concentration of four herbicides, it is concluded that A. variabilis is the most potent and promising cyanobacterial isolate as compared with other forms. This could be further improved by mutational techniques for exploitation as most potential and viable biofertilizer strain.

Evaluation of host-plant resistance of selected rice genotypes to the rice water weevil (Coleoptera:Curculionidae)

USDA-ARS?s Scientific Manuscript database

The rice water weevil (RWW), Lissorhoptrus oryzophilus Kuschel, is the most important economic insect pest of rice in the United States. Currently, management of RWW mainly depends upon the use of insecticides due to the lack of effective alternate management tactics. A three year field study was co...

Pathological Study of Blood Parasites in Rice Field Frogs, Hoplobatrachus rugulosus (Wiegmann, 1834)

PubMed Central

Sailasuta, Achariya; Satetasit, Jetjun; Chutmongkonkul, Malinee

2011-01-01

One hundred and forty adult rice field frogs, Hoplobatrachus rugulosus (Wiegmann, 1834), were collected in Srakaew province, Thailand. For blood parasite examination, thin blood smears were made and routinely stained with Giemsa. The results showed that 70% of the frogs (98/140) were infected with 5 species of blood parasites, including a Trypanosoma rotatorium-like organism, Trypanosoma chattoni, Hepatozoon sp. a, Hepatozoon sp. b, and Lankesterella minima. Pathological examination of the liver, lung, spleen, and kidney of the frogs that were apparently infected with one of these blood parasites were collected and processed by routine histology and subsequently stained with haematoxylin and eosin. Histopathological findings associated with the Trypanosoma rotatorium-like organism and Trypanosoma chattoni-infected frogs showed no pathological lesions. Hepatozoon sp. a and Hepatozoon sp. b-infected frogs developed inflammatory lesions predominantly in the liver, demonstrating granuloma-like lesions with Hepatozoon sp. meronts at the centre. Tissue sections of Lankesterella minima-infected frogs also showed lesions. Liver and spleen showed inflammatory lesions with an accumulation of melanomacrophage centres (MMCs) surrounding the meronts and merozoites. It is suggested that Hepatozoon sp. a, Hepatozoon sp. b, and Lankesterella minima-infections are capable of producing inflammatory lesions in the visceral organs of rice field frogs, and the severity of lesions is tentatively related to levels of parasitemia. PMID:21918731

Daily Rice Intake Strongly Influences the Incidence of Metabolic Syndrome in Japanese Men Aged 40–59 Years

PubMed Central

Watanabe, Yoko; Saito, Isao; Asada, Yasuhiko; Kishida, Taro; Matsuo, Tatsuhiro; Yamaizumi, Masamitsu; Kato, Tadahiro

2013-01-01

Objectives: The first objective of this study was to classify men aged 40–74 yrs with metabolic syndrome (MetS) according to daily rice intake, and the second was to investigate physical measurements, physiological examinations, blood biochemical assays, intake of food other than rice and lifestyle and environmental factors in the study group. Methods: We analyzed data from 6095 men aged 40–74 yrs who had undergone full medical examinations. The men were classified into 3 age groups: (1) 40–49 yrs, (2) 50–59 yrs, and (3) 60–74 yrs. The men were classified further into 3 groups according to daily rice intake: group 1 (≤300 g), group 2 (300–450 g), and group 3 (≥450 g). The relationship between daily rice intake and the following factors was analyzed in the three age brackets: (1) physical measurements including waist circumference, (2) physiological measurements, (3) serum biochemical indices, (4) whether or not the person was taking medication for hypertension, diabetes mellitus or serum lipid abnormalities, (5) lifestyle, and (6) consumption of foods other than rice. Results: Daily rice intake was related strongly to the occurrence of MetS in all three age brackets. Multiple logistic regression analysis showed (1) a significant increase in the odds ratio for MetS (1.461 times) for group 3 compared with group 1 in men aged 40–49 yrs and (2) a significant increase in the odds ratio for MetS (1.501 times) for group 3 compared with group 1 in men aged 50–59 yrs. However, there was no significant difference in the odds ratio for MetS among rice intake groups in the 60–74 age bracket. Conclusion: In men aged 40–59 yrs, daily rice intake strongly influenced the incidence of MetS, whereas in men aged 60–74 yrs, there was no relationship between daily rice intake and MetS. PMID:25649461

Genetic and field management strategies to for limiting accumulation of arsenic in rice grains

USDA-ARS?s Scientific Manuscript database

In 2002, high levels of arsenic were reported in rice produced in Bangladesh using soil and water naturally high in arsenic. Study of arsenic in rice produced in additional countries, including the USA, soon followed. Grain-arsenic is higher in rice than other crops because the flooding of rice pa...

Influence of nitrogen loading and plant nitrogen assimilation on nitrogen leaching and N₂O emission in forage rice paddy fields fertilized with liquid cattle waste.

PubMed

Riya, Shohei; Zhou, Sheng; Kobara, Yuso; Sagehashi, Masaki; Terada, Akihiko; Hosomi, Masaaki

2015-04-01

Livestock wastewater disposal onto rice paddy fields is a cost- and labor-effective way to treat wastewater and cultivate rice crops. We evaluated the influence of nitrogen loading rates on nitrogen assimilation by rice plants and on nitrogen losses (leaching and N2O emission) in forage rice fields receiving liquid cattle waste (LCW). Four forage rice fields were subjected to nitrogen loads of 107, 258, 522, and 786 kg N ha(-1) (N100, N250, N500, and N750, respectively) using basal fertilizer (chemical fertilizer) (50 kg N ha(-1)) and three LCW topdressings (each 57-284 kg N ha(-1)). Nitrogen assimilated by rice plants increased over time. However, after the third topdressing, the nitrogen content of the biomass did not increase in any treatment. Harvested aboveground biomass contained 93, 60, 33, and 31 % of applied nitrogen in N100, N250, N500, and N750, respectively. The NH4 (+) concentration in the pore water at a depth of 20 cm was less than 1 mg N L(-1) in N100, N250, and N500 throughout the cultivation period, while the NH4 (+) concentration in N750 increased to 3 mg N L(-1) after the third topdressing. Cumulative N2O emissions ranged from -0.042 to 2.39 kg N ha(-1); the highest value was observed in N750, followed by N500. In N750, N2O emitted during the final drainage accounted for 80 % of cumulative N2O emissions. This study suggested that 100-258 kg N ha(-1) is a recommended nitrogen loading rate for nitrogen recovery by rice plants without negative environmental impacts such as groundwater pollution and N2O emission.

Simulation of future global warming scenarios in rice paddies with an open-field warming facility

PubMed Central

2011-01-01

To simulate expected future global warming, hexagonal arrays of infrared heaters have previously been used to warm open-field canopies of upland crops such as wheat. Through the use of concrete-anchored posts, improved software, overhead wires, extensive grounding, and monitoring with a thermal camera, the technology was safely and reliably extended to paddy rice fields. The system maintained canopy temperature increases within 0.5°C of daytime and nighttime set-point differences of 1.3 and 2.7°C 67% of the time. PMID:22145582

Limited Fitness Advantages of Crop-Weed Hybrid Progeny Containing Insect-Resistant Transgenes (Bt/CpTI) in Transgenic Rice Field

PubMed Central

Yang, Xiao; Wang, Feng; Su, Jun; Lu, Bao-Rong

2012-01-01

Background The spread of insect-resistance transgenes from genetically engineered (GE) rice to its coexisting weedy rice (O. sativa f. spontanea) populations via gene flow creates a major concern for commercial GE rice cultivation. Transgene flow to weedy rice seems unavoidable. Therefore, characterization of potential fitness effect brought by the transgenes is essential to assess environmental consequences caused by crop-weed transgene flow. Methodology/Principal Findings Field performance of fitness-related traits was assessed in advanced hybrid progeny of F4 generation derived from a cross between an insect-resistant transgenic (Bt/CpTI) rice line and a weedy strain. The performance of transgene-positive hybrid progeny was compared with the transgene-negative progeny and weedy parent in pure and mixed planting of transgenic and nontransgenic plants under environmental conditions with natural vs. low insect pressure. Results showed that under natural insect pressure the insect-resistant transgenes could effectively suppress target insects and bring significantly increased fitness to transgenic plants in pure planting, compared with nontransgenic plants (including weedy parent). In contrast, no significant differences in fitness were detected under low insect pressure. However, such increase in fitness was not detected in the mixed planting of transgenic and nontransgenic plants due to significantly reduced insect pressure. Conclusions/Significance Insect-resistance transgenes may have limited fitness advantages to hybrid progeny resulted from crop-weed transgene flow owning to the significantly reduced ambient target insect pressure when an insect-resistant GE crop is grown. Given that the extensive cultivation of an insect-resistant GE crop will ultimately reduce the target insect pressure, the rapid spread of insect-resistance transgenes in weedy populations in commercial GE crop fields may be not likely to happen. PMID:22815975

Mapping paddy rice planting area in wheat-rice double-cropped areas through integration of Landsat-8 OLI, MODIS, and PALSAR images.

PubMed

Wang, Jie; Xiao, Xiangming; Qin, Yuanwei; Dong, Jinwei; Zhang, Geli; Kou, Weili; Jin, Cui; Zhou, Yuting; Zhang, Yao

2015-05-12

As farmland systems vary over space and time (season and year), accurate and updated maps of paddy rice are needed for studies of food security and environmental problems. We selected a wheat-rice double-cropped area from fragmented landscapes along the rural-urban complex (Jiangsu Province, China) and explored the potential utility of integrating time series optical images (Landsat-8, MODIS) and radar images (PALSAR) in mapping paddy rice planting areas. We first identified several main types of non-cropland land cover and then identified paddy rice fields by selecting pixels that were inundated only during paddy rice flooding periods. These key temporal windows were determined based on MODIS Land Surface Temperature and vegetation indices. The resultant paddy rice map was evaluated using regions of interest (ROIs) drawn from multiple high-resolution images, Google Earth, and in-situ cropland photos. The estimated overall accuracy and Kappa coefficient were 89.8% and 0.79, respectively. In comparison with the National Land Cover Data (China) from 2010, the resultant map better detected changes in the paddy rice fields and revealed more details about their distribution. These results demonstrate the efficacy of using images from multiple sources to generate paddy rice maps for two-crop rotation systems.

Novel Digital Features Discriminate Between Drought Resistant and Drought Sensitive Rice Under Controlled and Field Conditions.

PubMed

Duan, Lingfeng; Han, Jiwan; Guo, Zilong; Tu, Haifu; Yang, Peng; Zhang, Dong; Fan, Yuan; Chen, Guoxing; Xiong, Lizhong; Dai, Mingqiu; Williams, Kevin; Corke, Fiona; Doonan, John H; Yang, Wanneng

2018-01-01

Dynamic quantification of drought response is a key issue both for variety selection and for functional genetic study of rice drought resistance. Traditional assessment of drought resistance traits, such as stay-green and leaf-rolling, has utilized manual measurements, that are often subjective, error-prone, poorly quantified and time consuming. To relieve this phenotyping bottleneck, we demonstrate a feasible, robust and non-destructive method that dynamically quantifies response to drought, under both controlled and field conditions. Firstly, RGB images of individual rice plants at different growth points were analyzed to derive 4 features that were influenced by imposition of drought. These include a feature related to the ability to stay green, which we termed greenness plant area ratio (GPAR) and 3 shape descriptors [total plant area/bounding rectangle area ratio (TBR), perimeter area ratio (PAR) and total plant area/convex hull area ratio (TCR)]. Experiments showed that these 4 features were capable of discriminating reliably between drought resistant and drought sensitive accessions, and dynamically quantifying the drought response under controlled conditions across time (at either daily or half hourly time intervals). We compared the 3 shape descriptors and concluded that PAR was more robust and sensitive to leaf-rolling than the other shape descriptors. In addition, PAR and GPAR proved to be effective in quantification of drought response in the field. Moreover, the values obtained in field experiments using the collection of rice varieties were correlated with those derived from pot-based experiments. The general applicability of the algorithms is demonstrated by their ability to probe archival Miscanthus data previously collected on an independent platform. In conclusion, this image-based technology is robust providing a platform-independent tool for quantifying drought response that should be of general utility for breeding and functional genomics in

Depletion of rice as food of waterfowl wintering in the Mississippi Alluvial Valley

USGS Publications Warehouse

Greer, Danielle M.; Dugger, Bruce D.; Reinecke, Kenneth J.; Petrie, Mark J.

2009-01-01

Waterfowl habitat conservation strategies in the Mississippi Alluvial Valley (MAV) and several other wintering areas assume carrying capacity is limited by available food, and increasing food resources is an effective conservation goal. Because existing research on winter food abundance and depletion is insufficient to test this hypothesis, we used harvested rice fields as model foraging habitats to determine if waste rice seed is depleted before spring migration. We sampled rice fields (n = 39 [winter 2000-2001], n = 69 [2001-2002]) to estimate seed mass when waterfowl arrived in late autumn and departed in late winter. We also placed exclosures in subsets of fields in autumn (n = 8 [2000-2001], n = 20 [2001-2002]) and compared seed mass inside and outside exclosures in late winter to estimate rice depletion attributable to waterfowl and other processes. Finally, we used an experiment to determine if the extent of rice depletion differed among fields of varying initial abundance and if the seed mass at which waterfowl ceased foraging or abandoned fields differed from a hypothesized giving-up value of 50 kg/ha. Mean seed mass was greater in late autumn 2000 than 2001 (127.0 vs. 83.9 kg/ha; P = 0.018) but decreased more during winter 2000-2001 than 2001-2002 (91.3 vs. 55.7 kg/ha) and did not differ at the end of winter (35.8 vs. 28.3 kg/ha; P = 0.651). Assuming equal loss to deterioration inside and outside exclosures, we estimated waterfowl consumed 61.3 kg/ha (48.3%) of rice present in late autumn 2000 and 21.1 kg/ha (25.1%) in 2001. When we manipulated late-autumn rice abundance, mean giving-up mass of rice seed was similar among treatments (48.7 kg/ha; P = 0.205) and did not differ from 50 kg/ha (P = 0.726). We integrated results by constructing scenarios in which waterfowl consumed rice at different times in winter, consumption and deterioration were competing risks, and consumption occurred only above 50 kg/ha. Results indicated waterfowl likely consumed

Partitioning of CH4 and CO2 Production Originating from Rice Straw, Soil and Root Organic Carbon in Rice Microcosms

PubMed Central

Yuan, Quan; Pump, Judith; Conrad, Ralf

2012-01-01

Flooded rice fields are an important source of the greenhouse gas CH4. Possible carbon sources for CH4 and CO2 production in rice fields are soil organic matter (SOM), root organic carbon (ROC) and rice straw (RS), but partitioning of the flux between the different carbon sources is difficult. We conducted greenhouse experiments using soil microcosms planted with rice. The soil was amended with and without 13C-labeled RS, using two 13C-labeled RS treatments with equal RS (5 g kg−1 soil) but different δ13C of RS. This procedure allowed to determine the carbon flux from each of the three sources (SOM, ROC, RS) by determining the δ13C of CH4 and CO2 in the different incubations and from the δ13C of RS. Partitioning of carbon flux indicated that the contribution of ROC to CH4 production was 41% at tillering stage, increased with rice growth and was about 60% from the booting stage onwards. The contribution of ROC to CO2 was 43% at tillering stage, increased to around 70% at booting stage and stayed relatively constant afterwards. The contribution of RS was determined to be in a range of 12–24% for CH4 production and 11–31% for CO2 production; while the contribution of SOM was calculated to be 23–35% for CH4 production and 13–26% for CO2 production. The results indicate that ROC was the major source of CH4 though RS application greatly enhanced production and emission of CH4 in rice field soil. Our results also suggest that data of CH4 dissolved in rice field could be used as a proxy for the produced CH4 after tillering stage. PMID:23162678

Using natural Chinese zeolite to remove ammonium from rainfall runoff following urea fertilization of a paddy rice field.

PubMed

Wang, Xiao-Ling; Qiao, Bin; Li, Song-Min; Li, Jian-Sheng

2016-03-01

The potential of natural Chinese zeolite to remove ammonium from rainfall runoff following urea applications to a paddy rice field is assessed in this study. Laboratory batch kinetic and isotherm experiments were carried out first to investigate the ammonium adsorption capacity of the natural zeolite. Field experiments using zeolite adsorption barriers installed at drain outlets in a paddy rice field were also carried out during natural rainfall events to evaluate the barrier's dynamic removal capacity of ammonium. The results demonstrate that the adsorption kinetics are accurately described by the Elovich model, with a coefficient of determination (R (2)) ranging from 0.9705 to 0.9709, whereas the adsorption isotherm results indicate that the Langmuir-Freundlich model provides the best fit (R (2) = 0.992) for the equilibrium data. The field experiments show that both the flow rate and the barrier volume are important controls on ammonium removal from rainfall runoff. A low flow rate leads to a higher ammonium removal efficiency at the beginning of the tests, while a high flow rate leads to a higher quantity of ammonium adsorbed over the entire runoff process.

Do rice water weevils and rice stem borers compete when sharing a host plant?*

PubMed Central

Shi, Sheng-wei; He, Yan; Ji, Xiang-hua; Jiang, Ming-xing; Cheng, Jia-an

2008-01-01

The rice water weevil (RWW) Lissorhoptrus oryzophilus Kuschel (Coleoptera: Curculionidae) is an invasive insect pest of rice Oryza sativa L. in China. Little is known about the interactions of this weevil with indigenous herbivores. In the present study, adult feeding and population density of the weevil, injury level of striped stem borer Chilo suppressalis (Walker) (Lepidoptera: Pyralidae) and pink stem borer Sesamia inferens (Walker) (Lepidoptera: Noctuidae) to rice, as well as growth status of their host plants were surveyed in a rice field located in Southeastern Zhejiang, China, in 2004 with the objective to discover interspecific interactions on the rice. At tillering stage, both adult feeding of the weevil and injury of the stem borers tended to occur on larger tillers (bearing 5 leaves) compared with small tillers (bearing 2~4 leaves), but the insects showed no evident competition with each other. At booting stage, the stem borers caused more withering/dead hearts and the weevil reached a higher density on the plants which had more productive tillers and larger root system; the number of weevils per tiller correlated negatively with the percentage of withering/dead hearts of plants in a hill. These observations indicate that interspecific interactions exist between the rice water weevil and the rice stem borers with negative relations occurring at booting or earlier developmental stages of rice. PMID:18600788

Do rice water weevils and rice stem borers compete when sharing a host plant?

PubMed

Shi, Sheng-Wei; He, Yan; Ji, Xiang-Hua; Jiang, Ming-Xing; Cheng, Jia-An

2008-07-01

The rice water weevil (RWW) Lissorhoptrus oryzophilus Kuschel (Coleoptera: Curculionidae) is an invasive insect pest of rice Oryza sativa L. in China. Little is known about the interactions of this weevil with indigenous herbivores. In the present study, adult feeding and population density of the weevil, injury level of striped stem borer Chilo suppressalis (Walker) (Lepidoptera: Pyralidae) and pink stem borer Sesamia inferens (Walker) (Lepidoptera: Noctuidae) to rice, as well as growth status of their host plants were surveyed in a rice field located in Southeastern Zhejiang, China, in 2004 with the objective to discover interspecific interactions on the rice. At tillering stage, both adult feeding of the weevil and injury of the stem borers tended to occur on larger tillers (bearing 5 leaves) compared with small tillers (bearing 2~4 leaves), but the insects showed no evident competition with each other. At booting stage, the stem borers caused more withering/dead hearts and the weevil reached a higher density on the plants which had more productive tillers and larger root system; the number of weevils per tiller correlated negatively with the percentage of withering/dead hearts of plants in a hill. These observations indicate that interspecific interactions exist between the rice water weevil and the rice stem borers with negative relations occurring at booting or earlier developmental stages of rice.

Taste of Super-Dwarf Rice Cultured in Space

NASA Astrophysics Data System (ADS)

Hirai, Hiroaki; Kitaya, Yoshiaki

2016-07-01

The interest of food production for lunar base and manned Mars mission has increased recently. So far, plants cultured long duration in space were leafy vegetables, arabidopsis, wheat, barley and so on. Although rice is a staple food for most of the world, research on rice cultivation in space has not been done much. Rice grains are nutrient-rich with carbohydrate, protein and dietary fiber. Moreover, rice is a high yield crop and harvested grains have a long shelf life. Rice symbolizes the rice-eating culture of Japan, is extremely useful as a specific cultured plant candidate of Japan in space. In the previous report, 'Kozo-no-sumika' found from seedlings in raising of seedling was introduced as a super-dwarf rice to culture in space. Considering this rice as food in space, we investigate the taste characteristics of this rice. At present, waxy 'Kozo-no-sumika' and nonwaxy 'Hosetsu dwarf' of super-dwarf rice and 'Nipponbare' of previous standard rice for sensory test are cultured in paddy field. Hereafter, we will harvest rice, investigate yield, evaluate taste.

Single-wavelength based rice leaf color analyzer for nitrogen status estimation

NASA Astrophysics Data System (ADS)

Sumriddetchkajorn, Sarun; Intaravanne, Yuttana

2014-02-01

With the need of a tool for efficient nitrogen (N) fertilizer management in the rice field, this paper proposes a low-cost compact single-wavelength based colorimeter that can be used to indicate the specified six color levels of a rice leaf associated with the desired amount of N fertilizer for the rice field. Our key design is in a reflective optical architecture that allows us to investigate the amount of light scattered from only one side of the rice leaf. We also show how we implement this needed rice leaf color analyzer by integrating an off-the-shelf 562-nm wavelength light emitting diode (LED), a silicon photodiode, an 8-bit microcontroller, and a 6×1 LED panel in a compact plastic package. Field test results in rice fields confirm that leaf color levels of 1, 2, 3, 5, and 6 are effectively identified and their corresponding amount of N fertilizer can be determined. For the leaf color level of 4, our single-wavelength based rice leaf color analyzer sometimes indicates a higher color level of 5 whose suggested amount of N fertilizer is equal to that for the leaf color level of 4. Other key features include ease of use and upgradability for different color levels.

Mapping rice areas of South Asia using MODIS multitemporal data

NASA Astrophysics Data System (ADS)

Gumma, Murali Krishna; Nelson, Andrew; Thenkabail, Prasad S.; Singh, Amrendra N.

2011-01-01

Our goal is to map the rice areas of six South Asian countries using moderate-resolution imaging spectroradiometer (MODIS) time-series data for the time period 2000 to 2001. South Asia accounts for almost 40% of the world's harvested rice area and is also home to 74% of the population that lives on less than $2.00 a day. The population of the region is growing faster than its ability to produce rice. Thus, accurate and timely assessment of where and how rice is cultivated is important to craft food security and poverty alleviation strategies. We used a time series of eight-day, 500-m spatial resolution composite images from the MODIS sensor to produce rice maps and rice characteristics (e.g., intensity of cropping, cropping calendar) taking data for the years 2000 to 2001 and by adopting a suite of methods that include spectral matching techniques, decision trees, and ideal temporal profile data banks to rapidly identify and classify rice areas over large spatial extents. These methods are used in conjunction with ancillary spatial data sets (e.g., elevation, precipitation), national statistics, and maps, and a large volume of field-plot data. The resulting rice maps and statistics are compared against a subset of independent field-plot points and the best available subnational statistics on rice areas for the main crop growing season (kharif season). A fuzzy classification accuracy assessment for the 2000 to 2001 rice-map product, based on field-plot data, demonstrated accuracies from 67% to 100% for individual rice classes, with an overall accuracy of 80% for all classes. Most of the mixing was within rice classes. The derived physical rice area was highly correlated with the subnational statistics with R2 values of 97% at the district level and 99% at the state level for 2000 to 2001. These results suggest that the methods, approaches, algorithms, and data sets we used are ideal for rapid, accurate, and large-scale mapping of paddy rice as well as for generating

Characterization and differential expression patterns of conserved microRNAs and mRNAs in three genders of the rice field eel (Monopterus albus).

PubMed

Gao, Yu; Guo, Wei; Hu, Qing; Zou, Ming; Tang, Rong; Chi, Wei; Li, Dapeng

2014-01-01

MicroRNAs (miRNAs) are endogenous small RNAs that can regulate target mRNAs by binding to their sequences in the 3' untranslated region. The expression of miRNAs and their biogenetic pathway are involved in sexual differentiation and in the regulation of the development of germ cells and gonadal somatic cells. The rice field eel (Monopterus albus) undergoes a natural sexual transformation from female to male via an intersex stage during its life cycle. To investigate the molecular mechanisms of this sexual transformation, miRNAs present in the different sexual stages of the rice field eel were identified by high-throughput sequencing technology. A significantly differential expression among the 3 genders (p < 0.001) was observed for 48 unique miRNAs and 3 miRNAs*. Only 9 unique miRNAs showed a more than 8-fold change in their expression among the 3 genders, including mal-miR-430a and mal-miR-430c which were higher in females than in males. However, mal-miR-430b was only detected in males. Several potential miRNA target genes (cyp19a, cyp19b, nr5a1b, foxl2 amh, and vasa) were also investigated. Real-time RT-PCR demonstrated highly specific expression patterns of these genes in the 3 genders of the rice field eel. Many of these genes are targets of mal-miR-430b according to the TargetScan and miRTarBase. These results suggest that the miR-430 family may be involved in the sexual transformation of the rice field eel. © 2014 S. Karger AG, Basel.

Interference of allelopathic rice with penoxsulam-resistant barnyardgrass.

PubMed

Yang, Xue-Fang; Kong, Chui-Hua; Yang, Xia; Li, Yong-Feng

2017-11-01

Despite increasing knowledge of allelopathic rice interference with barnyardgrass, relatively little is known about its action on herbicide-resistant barnyardgrass. The incidence of herbicide-resistant barnyardgrass is escalating in paddy fields. Knowledge of the interference of allelopathic rice with herbicide-resistant barnyardgrass and the potential mechanisms involved is warranted. Penoxsulam-resistant and -susceptible barnyardgrass biotypes were identified and segregated from a putative penoxsulam-resistant population occurring in paddy fields in China. Allelopathic rice inhibited the growth of barnyardgrass roots more than shoots, regardless of biotype. In particular, there was a stronger inhibition for resistant barnyardgrass than for susceptible barnyardgrass. Allelopathic rice significantly reduced total root length, total root area, maximum root amplitude and maximum root depth in barnyardgrass. Furthermore, the rice allelochemicals tricin and momilactone B inhibited the growth of both resistant and susceptible barnyardgrass. Compared with root contact, root segregation significantly increased inhibition of barnyardgrass with an increase in rice allelochemicals. Root exudates from barnyardgrass induced the production of rice allelochemicals, but the effect of susceptible barnyardgrass was much stronger than that of resistant barnyardgrass. Allelopathic rice can interfere with the growth of penoxsulam-resistant barnyardgrass through allelochemical-mediated root interactions. This type of allelopathic interference may provide a non-herbicidal alternative for herbicide-resistant weed management in paddy systems. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

Molecular evolution of flowering time loci in U.S. weedy rice

USDA-ARS?s Scientific Manuscript database

Weedy rice is a persistent weed of cultivated rice (Oryza sativa) fields worldwide, which competes with the crop and drastically reduces rice yields. Within the US, two main populations of genetically differentiated weedy rice exist, the straw-hulled (SH) group and the black-hulled awned (BHA) grou...

Mitigation options for methane emissions from rice fields in the Philippines

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lantin, R.S.; Buendia, L.V.; Wassmann, R.

1996-12-31

The contribution of Philippine rice production to global methane emission and breakthroughs in methane emission studies conducted in the country are presented in this paper. A significant impact in the reduction of GHG emissions from agriculture can be achieved if methane emissions from ricefields can be abated. This study presents the contribution of Philippine rice cultivation to global methane emission and breakthroughs in methane emission studies in the country which address the issue of mitigation. Using the derived emission factors from local measurements, rice cultivation contributes 566.6 Gg of methane emission in the Philippines. This value is 62% of themore » total methane emitted from the agriculture sector. The emission factors employed which are 78% of the IPCC value for irrigated rice and 95% for rainfed rice were derived from measurements with an automatic system taken during the growth duration in the respective ecosystems. Plots drained for 2 weeks at midtillering and before harvest gave a significant reduction in methane emission as opposed to continuously flooded plots and plots drained before harvest. The cultivar Magat reduced methane emission by 50% as compared to the check variety IR72. The application of ammonium sulfate instead of urea reduced methane emission by 10% to 34%. Addition of 6 t ha{sup {minus}1} phosphogypsum in combination with urea reduced emission by 74% as opposed to plots applied with urea alone. It is also from the results of such measurements that abatement strategies are based as regards to modifying treatments such as water management, fertilization, and choice of rice variety. It is not easy to identify and recommend mitigation strategies that will fit a particular cropping system. However, the identified mitigation options provide focus for the abatement of methane emission from ricefields.« less

Integrated rice-duck farming mitigates the global warming potential in rice season.

PubMed

Xu, Guochun; Liu, Xin; Wang, Qiangsheng; Yu, Xichen; Hang, Yuhao

2017-01-01

Integrated rice-duck farming (IRDF), as a mode of ecological agriculture, is an important way to realize sustainable development of agriculture. A 2-year split-plot field experiment was performed to evaluate the effects of IRDF on methane (CH 4 ) and nitrous oxide (N 2 O) emissions and its ecological mechanism in rice season. This experiment was conducted with two rice farming systems (FS) of IRDF and conventional farming (CF) under four paddy-upland rotation systems (PUR): rice-fallow (RF), annual straw incorporating in rice-wheat rotation system (RWS), annual straw-based biogas residues incorporating in rice-wheat rotation system (RWB), and rice-green manure (RGM). During the rice growing seasons, IRDF decreased the CH 4 emission by 8.80-16.68%, while increased the N 2 O emission by 4.23-15.20%, when compared to CF. Given that CH 4 emission contributed to 85.83-96.22% of global warming potential (GWP), the strong reduction in CH 4 emission led to a significantly lower GWP of IRDF as compared to CF. The reason for this trend was because IRDF has significant effect on dissolved oxygen (DO) and soil redox potential (Eh), which were two pivotal factors for CH 4 and N 2 O emissions in this study. The IRDF not only mitigates the GWP, but also increases the rice yield by 0.76-2.43% compared to CF. Moreover, compared to RWS system, RF, RWB and RGM systems significantly reduced CH 4 emission by 50.17%, 44.89% and 39.51%, respectively, while increased N 2 O emission by 10.58%, 14.60% and 23.90%, respectively. And RWS system had the highest GWP. These findings suggest that mitigating GWP and improving rice yield could be simultaneously achieved by the IRDF, and employing suitable PUR would benefit for relieving greenhouse effect. Copyright © 2016 Elsevier B.V. All rights reserved.

Water management practices affect arsenic and cadmium accumulation in rice grains.

PubMed

Sun, Liming; Zheng, Manman; Liu, Hongyan; Peng, Shaobing; Huang, Jianliang; Cui, Kehui; Nie, Lixiao

2014-01-01

Cadmium (Cd) and arsenic (As) accumulation in rice grains is a great threat to its productivity, grain quality, and thus human health. Pot and field studies were carried out to unravel the effect of different water management practices (aerobic, aerobic-flooded, and flooded) on Cd and As accumulation in rice grains of two different varieties. In pot experiment, Cd or As was also added into the soil as treatment. Pots without Cd or As addition were maintained as control. Results indicated that water management practices significantly influenced the Cd and As concentration in rice grains and aerobic cultivation of rice furnished less As concentration in its grains. Nonetheless, Cd concentration in this treatment was higher than the grains of flooded rice. Likewise, in field study, aerobic and flooded rice cultivation recorded higher Cd and As concentration, respectively. However, growing of rice in aerobic-flooded conditions decreased the Cd concentration by 9.38 times on average basis as compared to aerobic rice. Furthermore, this treatment showed 28% less As concentration than that recorded in flooded rice cultivation. The results suggested that aerobic-flooded cultivation may be a promising strategy to reduce the Cd and As accumulations in rice grains simultaneously.

[Effects of controlled release blend bulk urea on soil nitrogen and soil enzyme activity in wheat and rice fields].

PubMed

Zhang, Jing Sheng; Wang, Chang Quan; Li, Bing; Liang, Jing Yue; He, Jie; Xiang, Hao; Yin, Bin; Luo, Jing

2017-06-18

A field experiment was conducted to investigate the effect of controlled-release fertilizer (CRF) combined with urea (UR) on the soil fertility and environment in wheat-rice rotation system. Changes in four forms of nitrogen (total nitrogen, ammonium nitrogen, nitrate nitrogen, and microbial biomass nitrogen) and in activities of three soil enzymes participating in nitrogen transformation (urease, protease, and nitrate reductase) were measured in seven fertilization treatments (no fertilization, routine fertilization, 10%CRF+90%UR, 20%CRF+80%UR, 40%CRF+60%UR, 80%CRF+20%UR, and 100%CRF). The results showed that soil total nitrogen was stable in the whole growth period of wheat and rice. There was no significant difference among the treatments of over 20% CRF in soil total nitrogen content of wheat and rice. The soil inorganic nitrogen content was increased dramatically in treatments of 40% or above CRF during the mid-late growing stages of wheat and rice. With the advance of the growth period, conventional fertilization significantly decreased soil microbial biomass nitrogen, but the treatments of 40% and above CRF increased the soil microbial biomass nitrogen significantly. The soil enzyme activities were increased with over 40% of CRF in the mid-late growing stage of wheat and rice. By increasing the CRF ratio, the soil protease activity and nitrate reductase activity were improved gradually, and peaked in 100% CRF. The treatments of above 20% CRF could decrease the urease activity in tillering stage of rice and delay the peak of ammonium nitrogen, which would benefit nitrogen loss reduction. The treatments of 40% and above CRF were beneficial to improving soil nitrogen supply and enhancing soil urease and protease activities, which could promote the effectiveness of nitrogen during the later growth stages of wheat and rice. The 100% CRF treatment improved the nitrate reductase activity significantly during the later stage of wheat and rice. Compared with the

Concentrated Protein Body Product Derived from Rice Endosperm as an Oral Tolerogen for Allergen-Specific Immunotherapy—A New Mucosal Vaccine Formulation against Japanese Cedar Pollen Allergy

PubMed Central

Wakasa, Yuhya; Takagi, Hidenori; Watanabe, Nobumasa; Kitamura, Noriko; Fujiwara, Yoshihiro; Ogo, Yuko; Hayashi, Shimpei; Yang, Lijun; Ohta, Masaru; Thet Tin, Wai Wai; Sekikawa, Kenji; Takano, Makoto; Ozawa, Kenjirou; Hiroi, Takachika; Takaiwa, Fumio

2015-01-01

The endoplasmic reticulum-derived type-I protein body (PB-I) from rice endosperm cells is an ideal candidate formulation for the oral delivery of bioencapsulated peptides as tolerogens for allergen-specific immunotherapy. In the present study, PBs containing the deconstructed Japanese cedar pollen allergens Cryptomeria japonica 1 (Cry j 1) and Cry j 2 were concentrated by treatment with thermostable α-amylase at 90°C to remove the starch from milled rice powder, which resulted in a 12.5-fold reduction of dry weight compared to the starting material. The modified Cry j 1 and Cry j 2 antigens in this concentrated PB product were more resistant to enzymatic digestion than those in the milled seed powder despite the absence of intact cell wall and starch, and remained stable for at least 10 months at room temperature without detectable loss or degradation. The high resistance of these allergens could be attributed to changes in protein physicochemical properties induced by the high temperature concentration process, as suggested by the decreased solubility of the antigens and seed proteins in PBs in step-wise-extraction experiments. Confocal microscopy showed that the morphology of antigen-containing PB-Is was preserved in the concentrated PB product. The concentrated PB product induced specific immune tolerance against Cry j 1 and Cry j 2 in mice when orally administered, supporting its potential use as a novel oral tolerogen formulation. PMID:25774686

A GIS-based Upscaling Estimation of Nutrient Runoff Losses from Rice Paddy Fields to a Regional Level.

PubMed

Sun, Xiaoxiao; Liang, Xinqiang; Zhang, Feng; Fu, Chaodong

2016-11-01

Nutrient runoff losses from cropping fields can lead to nonpoint source pollution; however, the level of nutrient export is difficult to evaluate, particularly at the regional scale. This study aimed to establish a novel yet simple approach for estimating total nitrogen (TN) and total phosphorus (TP) runoff losses from regional paddy fields. In this approach, temporal changes of nutrient concentrations in floodwater were coupled with runoff-processing functions in rice ( L.) fields to calculate nutrient runoff losses for three site-specific field experiments. Validation experiments verified the accuracy of this method. The geographic information system technique was used to upscale and visualize the TN and TP runoff losses from field to regional scales. The results indicated that nutrient runoff losses had significant spatio-temporal variation characteristics during rice seasons, which were positively related to fertilizer rate and precipitation. The average runoff losses over five study seasons were 20.21 kg N ha for TN and 0.76 kg P ha for TP. Scenario analysis showed that TN and TP losses dropped by 7.64 and 3.0%, respectively, for each 10% reduction of fertilizer input. For alternate wetting and drying water management, the corresponding reduction ratio was 24.7 and 14.0% respectively. Our results suggest that, although both water and fertilizer management can mitigate nutrient runoff losses, the former is significantly more effective. Copyright © by the American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America, Inc.

The impact of herbicide-resistant rice technology on phenotypic diversity and population structure of United States weedy rice.

PubMed

Burgos, Nilda Roma; Singh, Vijay; Tseng, Te Ming; Black, Howard; Young, Nelson D; Huang, Zhongyun; Hyma, Katie E; Gealy, David R; Caicedo, Ana L

2014-11-01

The use of herbicide-resistant (HR) Clearfield rice (Oryza sativa) to control weedy rice has increased in the past 12 years to constitute about 60% of rice acreage in Arkansas, where most U.S. rice is grown. To assess the impact of HR cultivated rice on the herbicide resistance and population structure of weedy rice, weedy samples were collected from commercial fields with a history of Clearfield rice. Panicles from each weedy type were harvested and tested for resistance to imazethapyr. The majority of plants sampled had at least 20% resistant offspring. These resistant weeds were 97 to 199 cm tall and initiated flowering from 78 to 128 d, generally later than recorded for accessions collected prior to the widespread use of Clearfield rice (i.e. historical accessions). Whereas the majority (70%) of historical accessions had straw-colored hulls, only 30% of contemporary HR weedy rice had straw-colored hulls. Analysis of genotyping-by-sequencing data showed that HR weeds were not genetically structured according to hull color, whereas historical weedy rice was separated into straw-hull and black-hull populations. A significant portion of the local rice crop genome was introgressed into HR weedy rice, which was rare in historical weedy accessions. Admixture analyses showed that HR weeds tend to possess crop haplotypes in the portion of chromosome 2 containing the ACETOLACTATE SYNTHASE gene, which confers herbicide resistance to Clearfield rice. Thus, U.S. HR weedy rice is a distinct population relative to historical weedy rice and shows modifications in morphology and phenology that are relevant to weed management. © 2014 American Society of Plant Biologists. All Rights Reserved.

Basic Restriction and Reference Level in Anatomically-based Japanese Models for Low-Frequency Electric and Magnetic Field Exposures

NASA Astrophysics Data System (ADS)

Takano, Yukinori; Hirata, Akimasa; Fujiwara, Osamu

Human exposed to electric and/or magnetic fields at low frequencies may cause direct effect such as nerve stimulation and excitation. Therefore, basic restriction is regulated in terms of induced current density in the ICNIRP guidelines and in-situ electric field in the IEEE standard. External electric or magnetic field which does not produce induced quantities exceeding the basic restriction is used as a reference level. The relationship between the basic restriction and reference level for low-frequency electric and magnetic fields has been investigated using European anatomic models, while limited for Japanese model, especially for electric field exposures. In addition, that relationship has not well been discussed. In the present study, we calculated the induced quantities in anatomic Japanese male and female models exposed to electric and magnetic fields at reference level. A quasi static finite-difference time-domain (FDTD) method was applied to analyze this problem. As a result, spatially averaged induced current density was found to be more sensitive to averaging algorithms than that of in-situ electric field. For electric and magnetic field exposure at the ICNIRP reference level, the maximum values of the induced current density for different averaging algorithm were smaller than the basic restriction for most cases. For exposures at the reference level in the IEEE standard, the maximum electric fields in the brain were larger than the basic restriction in the brain while smaller for the spinal cord and heart.

Characteristics of the Japanese Diet Described in Epidemiologic Publications: A Qualitative Systematic Review.

PubMed

Suzuki, Nozomu; Goto, Yoshihito; Ota, Haruka; Kito, Kumiko; Mano, Fumika; Joo, Erina; Ikeda, Kaori; Inagaki, Nobuya; Nakayama, Takeo

2018-01-01

International interest in the Japanese diet has grown in recent years. The aim of this systematic review was to evaluate and organize the Japanese diet and dietary characteristics from an epidemiological perspective, mainly focusing on the nutritional and dietary elements. PubMed, Web of Science, Japan Medical Abstracts Society, JDream III, and CiNii databases were searched. The eligibility criteria included research with an epidemiological study design that was either cross-sectional, cohort, or case-control-based that defined the dietary patterns of the Japanese diet using dietary pattern analysis. A total of 39 research articles that described the Japanese diet were included. The data that were extracted included the following: implementing country, location, study design, participant characteristics, key outcomes, methods used in the analysis of dietary patterns, and descriptions of the Japanese diet. As a result of the systematic review analyzing the descriptions of the Japanese diet from 39 selected articles, we were able to aggregate the descriptions into 16 categories from 33 factors. After performing a content analysis using a further aggregation of categories, we found that the top three applicable categories were soybeans/soybean-derived products, seafood, and vegetables; these were followed by rice and miso soup. The Japanese dietary content was found to be diverse based on an examination of epidemiological studies; however, we were able to aggregate the content into 16 categories. The Japanese diet is considered to be a dietary pattern that contains a combination of factors: the dietary staple, side dishes, and soup.

Molecular evolution of the sh4 shattering locus in U.S. weedy rice

USDA-ARS?s Scientific Manuscript database

Cultivated rice fields worldwide are plagued with weedy rice, a congeneric weed of cultivated rice (Oryza sativa L.). The persistence of weedy rice has been attributed, in part, to its ability to shatter (disperse) seed prior to crop harvesting. In the United States, separately evolved weedy rice g...

Evaluating the non-rice host plant species of Sesamia inferens (Lepidoptera: Noctuidae) as natural refuges: resistance management of Bt rice.

PubMed

Liu, Zhuorong; Gao, Yulin; Luo, Ju; Lai, Fengxiang; Li, Yunhe; Fu, Qiang; Peng, Yufa

2011-06-01

Although rice (Oryza sativa L.) lines that express Bacillus thuringiensis (Bt) toxins have shown great potential for managing the major Lepidoptera pests of rice in southern China, including Sesamia inferens, their long-term use is dependent on managing resistance development to Bt toxins in pest populations. The maintenance of "natural" refuges, non-Bt expressing plants that are hosts for a target pest, has been proposed as a means to minimize the evolution of resistance to Bt toxins in transgenic plants. In the current study, field surveys and greenhouse experiments were conducted to identify host plants of S. inferens that could serve as "natural" refuges in rice growing areas of southern China. A field survey showed that 34 plant species in four families can be alternative host plants of S. inferens. Based on injury level under field conditions, rice (Oryza sativa L.); water oat (Zizania latifolia Griseb.); corn (Zea mays L.); tidalmarsh flatsedge (Cyperus serotinus Rottb.); and narrow-leaved cat-tail (Typha angustifolia Linn.) were identified as the primary host plant species of S. inferens. Greenhouse experiments further demonstrated that water oat, corn, and narrow-leaved cat-tail could support the survival and development of S. inferens. Interestingly, greenhouse experiments showed that S. inferens preferred to lay eggs on tidalmarsh flatsedge compared with the other three nonrice host species, although no pupae were found in the plants examined in field surveys. Few larvae were found to survive on tidalmarsh flatsedge in greenhouse bioassays, suggesting that tidalmarsh flatsedge could serve as a "dead-end" trap crop for S. inferens, but is not a candidate to serve as natural refuge to maintain susceptible S. inferens. Overall, these results suggest that water-oat, corn, and narrow-leaved cat-tail might serve as "natural refuge" for S. inferens in rice planting area of southern China when Bt rice varieties are planted.

Biogeochemical cycling in Rice Agroecosystems Resulting From Water and Si management: Implications for As abatement and Sustainable Rice Production

NASA Astrophysics Data System (ADS)

Seyfferth, A.; Limmer, M. A.; Amaral, D.; Teasley, W.

2017-12-01

Flooded rice agroecosystems favor geochemical conditions that mobilize soil-bound arsenic (As) and produce methane (CH4). These negative outcomes of flooded rice may lead to As exposure upon As-laden rice grain consumption and enhanced greenhouse gas emissions. Periodic draining of fields (e.g., alternate wetting and drying) is effective at minimizing these negative outcomes, but may reduce rice yield, increase toxic Cd in grain, and increase nitrous oxide (N2O) emissions. Because 3 of the 4 dominant chemical form of As in flooded paddy soil share the efficient Si uptake pathway, increasing plant-available Si can decrease toxic As in grain and boost yield, particularly when plants are stressed with As. We used combined pot and field studies to examine the biogeochemical cycling of As, Fe, Si, and C when plants are grown with water and/or Si management, the latter of which under both low and high As conditions. We show that increasing plant-available Si can be used alone or in conjunction with water management to improve rice yields depending on the edaphic conditions. These processes and findings will be discussed in the larger context of global food security.

Spatial Analysis of Rice Blast in China at Three Different Scales.

PubMed

Guo, Fangfang; Chen, Xinglong; Lu, Minghong; Yang, Li; Wang, Shi Wei; Wu, Bo Ming

2018-05-22

In this study, spatial analyses were conducted at three different scales to better understand the epidemiology of rice blast, a major rice disease caused by Magnaporthe oryzae. At regional scale, across the major rice production regions in China, rice blast incidence was monitored on 101 dates at 193 stations from June 10 th to Sep. 10 th during 2009-2014, and surveyed in 143 fields in September, 2016; at county scale, 3 surveys were done covering 1-5 counties in 2015-2016; and at field scale, blast was evaluated in 6 fields in 2015-2016. Spatial cluster and hot spot analyses were conducted in GIS on the geographical pattern of the disease at regional scale, and geostatistical analysis performed at all the three scales. Cluster and hot spot analyses revealed that high-disease areas were clustered in mountainous areas in China. Geostatistical analyses detected spatial dependence of blast incidence with influence ranges of 399 to 1080 km at regional scale, and 5 to 10 m at field scale, but not at county scale. The spatial patterns at different scales might be determined by inherent properties of rice blast and environmental driving forces, and findings from this study provide helpful information to sampling and management of rice blast.

Taxocoenosis and distribution of nektonic fauna in the rice fields of Kashmir (J and K) India.

PubMed

Bahaar, S W N; Bhat, G A

2011-04-15

Present study attempts to identify the taxocoenosis and distribution of nektonic fauna harbouring the rice field ecosystems of Kashmir. The main objective of the study was to provide an overview of the nektonic community composition and physicochemical characteristics of flood waters. 6 sites were selected in Kupwara, Bandipora, Budgam, Srinagar, Pulwama and Anantnag districts of valley Kashmir. A total of 26 taxa belonging to 13 different orders were reported during the study which commenced through 2 consecutive crop cycles. The taxocoenosis was dominated by Coleoptera (10 taxa) followed by Hemiptera (3 taxa), Diptera (2 taxa), Diplostraca (2 taxa), Acarina, Anostraca, Anura, Amphipoda, Basommatophora, Cypriniformes, Cyprinodontiformes, Odonata and Pulmonata (1 taxa each). Diversity was calculated using Simpsons Index (D), Simpsons Index of Diversity (1-D), Simpsons Reciprocal Index (1/D), Shannon-Weiner Index (H'), Margalef Richness Index (d) and Evenness Index (e). Kupwara (34 degrees 02'N; 74 degrees 16'E) formed the most diverse site registering a total of 2384 individuals belonging to 24 taxa. A perusal of the primary data related to the physicochemical attributes of flood waters exhibited that average water temperature varied between 19-30 degrees C, average air temperature varied between 21 and 33 degrees C. pH depicted a variation between 6.0 and 9.0, Dissolved Oxygen varied between a minimum of 1.0 mg L(-1) and a maximum of 10 mg L(-1). Free CO2 ranged between 0 mg L(-1) and 6.1 mg(-1). The results pressed the need for recognizing and preserving rice fields as potential habitats for organisms that have successfully adapted to the highly manipulated and eutrophic conditions of rice paddies.

Community structure of methanogenic archaea and methane production associated with compost-treated tropical rice-field soil.

PubMed

Singh, Alpana; Singh, Ram S; Upadhyay, Siddh N; Joshi, Chaitanya G; Tripathi, Ajay K; Dubey, Suresh K

2012-10-01

The diversity and density of methanogenic archaea and methane production were investigated ex situ at different growth stages of rice plant cultivated in compost-treated tropical rice fields. The qPCR analysis revealed variation in methanogens population from 3.40 × 10(6) to 1.11 × 10(7)  copies g(-1)  dws, in the year 2009 and 4.37 × 10(6) to 1.36 × 10(7)  copies g(-1)  dws in the year 2010. Apart from methanogens, a large number of bacterial (9.60 × 10(9) -1.44 × 10(10)  copies g(-1)  dws) and archaeal (7.13 × 10(7) -3.02 × 10(8)  copies g(-1)  dws) communities were also associated with methanogenesis. Methanogen population size varied in the order: flowering > ripening > tillering > postharvest > preplantation stage. The RFLP-based 16S rRNA gene-targeted phylogenetic analysis showed that clones were closely related to diverse group of methanogens comprising members of Methanomicrobiaceae, Methanosarcinaceae, Methanosaetaceae and RC I. Laboratory incubation studies revealed higher amount of cumulative CH(4) at the flowering stage. The integration of methanogenic community structure and CH(4) production potential of soil resulted in a better understanding of the dynamics of CH(4) production in organically treated rice-field soil. The hypothesis that the stages of plant development influence the methanogenic community structure leading to temporal variation in the CH(4) production has been successfully tested. © 2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.

Mapping rice areas of South Asia using MODIS multitemporal data

USGS Publications Warehouse

Gumma, M.K.; Nelson, A.; Thenkabail, P.S.; Singh, A.N.

2011-01-01

Our goal is to map the rice areas of six South Asian countries using moderate-resolution imaging spectroradiometer (MODIS) time-series data for the time period 2000 to 2001. South Asia accounts for almost 40% of the world's harvested rice area and is also home to 74% of the population that lives on less than $2.00 a day. The population of the region is growing faster than its ability to produce rice. Thus, accurate and timely assessment of where and how rice is cultivated is important to craft food security and poverty alleviation strategies. We used a time series of eight-day, 500-m spatial resolution composite images from the MODIS sensor to produce rice maps and rice characteristics (e.g., intensity of cropping, cropping calendar) taking data for the years 2000 to 2001 and by adopting a suite of methods that include spectral matching techniques, decision trees, and ideal temporal profile data banks to rapidly identify and classify rice areas over large spatial extents. These methods are used in conjunction with ancillary spatial data sets (e.g., elevation, precipitation), national statistics, and maps, and a large volume of field-plot data. The resulting rice maps and statistics are compared against a subset of independent field-plot points and the best available subnational statistics on rice areas for the main crop growing season (kharif season). A fuzzy classification accuracy assessment for the 2000 to 2001 rice-map product, based on field-plot data, demonstrated accuracies from 67% to 100% for individual rice classes, with an overall accuracy of 80% for all classes. Most of the mixing was within rice classes. The derived physical rice area was highly correlated with the subnational statistics with R2 values of 97% at the district level and 99% at the state level for 2000 to 2001. These results suggest that the methods, approaches, algorithms, and data sets we used are ideal for rapid, accurate, and large-scale mapping of paddy rice as well as for generating

Genetic dissection of grain traits in Yamadanishiki, an excellent sake-brewing rice cultivar.

PubMed

Okada, Satoshi; Suehiro, Miki; Ebana, Kaworu; Hori, Kiyosumi; Onogi, Akio; Iwata, Hiroyoshi; Yamasaki, Masanori

2017-12-01

The grain traits of Yamadanishiki, an excellent sake-brewing rice cultivar in Japan, are governed by multiple QTLs, namely, a total of 42 QTLs including six major QTLs. Japanese rice wine (sake) is produced using brewing rice (Oryza sativa L.) that carries traits desirable for sake-brewing, such as a larger grain size and higher white-core expression rate (WCE) compared to cooking rice cultivars. However, the genetic basis for these traits in brewing rice cultivars is still unclear. We performed analyses of quantitative trait locus (QTL) of grain and days to heading over 3 years on populations derived from crosses between Koshihikari, a cooking rice, and Yamadanishiki, an excellent sake-brewing rice. A total of 42 QTLs were detected for the grain traits, and the Yamadanishiki alleles at 16 QTLs contributed to larger grain size. Two major QTLs essential for regulating both 100-grain weight (GWt) and grain width (GWh) were harbored in the same regions on chromosomes 5 and 10. An interaction was noted between the environment and the QTL associated with WCE on chromosome 6, which was detected in two of 3 years. In addition, two QTLs for WCE on chromosomes 3 and 10 overlapped with the QTLs for GWt and GWh, suggesting that QTLs associated with grain size also play an important role in the formation of white-core. Despite differences in the rate of grain growth in both Koshihikari and Yamadanishiki across 2 years, the WCE in Yamadanishiki remained consistent, thus demonstrating that the formation of white-core does not depend on grain filling speed. These data can be informative for programs involved in breeding better cooking and brewing rice cultivars.

Reducing rice field algae and cyanobacteria abundance by altering phosphorus fertilizer applications

USDA-ARS?s Scientific Manuscript database

In California’s water seeded rice systems algal/cyanobacterial biomass can be a problem during rice establishment. Algal/cyanobacterial growth may be stimulated by phosphorus (P) additions in freshwater habitats, so we set up experiments to evaluate the effects of fertilizer P management on algal/cy...

Soil to rice transfer factors for (226)Ra, (228)Ra, (210)Pb, (40)K and (137)Cs: a study on rice grown in India.

PubMed

Karunakara, N; Rao, Chetan; Ujwal, P; Yashodhara, I; Kumara, Sudeep; Ravi, P M

2013-04-01

India is the second largest producer of rice (Oryza sativa L.) in the world and rice is an essential component of the diet for a majority of the population in India. However, detailed studies aimed at the evaluation of radionuclide transfer factors (F(v)) for the rice grown in India are almost non-existent. This paper presents the soil to rice transfer factors for natural ((226)Ra, (228)Ra, (40)K, and (210)Pb) and artificial ((137)Cs) radionuclides for rice grown in natural field conditions on the West Coast of India. A rice field was developed very close to the Kaiga nuclear power plant and the water required for this field was drawn from the cooling water discharge canal of the power plant. For a comparative study of the radionuclide transfer factors, rice samples were also collected from the rice fields of nearby villages. The study showed that the (226)Ra and (228)Ra activity concentrations were below detection levels in different organs of the rice plant. The soil to un-hulled rice grain (40)K transfer factor varied in the range of 6.5 × 10(-1) to 2.9 with a mean of 0.15 × 10(1), and of (210)Pb varied in the range of <1.2 × 10(-2) to 8.1 × 10(-1) with a mean of 1.4 × 10(-1), and of (137)Cs varied in the range of 6.6 × 10(-2) to 3.4 × 10(-1) with a mean of 2.1 × 10(-1). The mean values of un-hulled grain to white rice processing retention factors (F(r)) were 0.12 for (40)K, 0.03 for (210)Pb, and 0.14 for (137)Cs. Using these processing retention factors, the soil to white rice transfer factors were estimated and these were found to have mean values of 1.8 × 10(-1), 4.2 × 10(-3), and 3.0 × 10(-2) for (40)K, (210)Pb, and (137)Cs, respectively. The study has shown that the transfer of (40)K was higher for above the ground organs than for the root, but (210)Pb and (137)Cs were retained in the root and their transfer to above the ground organs of the rice plant is significantly lower. Copyright © 2012 Elsevier Ltd. All rights

Multiple abiotic stimuli are integrated in the regulation of rice gene expression under field conditions.

PubMed

Plessis, Anne; Hafemeister, Christoph; Wilkins, Olivia; Gonzaga, Zennia Jean; Meyer, Rachel Sarah; Pires, Inês; Müller, Christian; Septiningsih, Endang M; Bonneau, Richard; Purugganan, Michael

2015-11-26

Plants rely on transcriptional dynamics to respond to multiple climatic fluctuations and contexts in nature. We analyzed the genome-wide gene expression patterns of rice (Oryza sativa) growing in rainfed and irrigated fields during two distinct tropical seasons and determined simple linear models that relate transcriptomic variation to climatic fluctuations. These models combine multiple environmental parameters to account for patterns of expression in the field of co-expressed gene clusters. We examined the similarities of our environmental models between tropical and temperate field conditions, using previously published data. We found that field type and macroclimate had broad impacts on transcriptional responses to environmental fluctuations, especially for genes involved in photosynthesis and development. Nevertheless, variation in solar radiation and temperature at the timescale of hours had reproducible effects across environmental contexts. These results provide a basis for broad-based predictive modeling of plant gene expression in the field.

Human health risk assessment, congener specific analysis and spatial distribution pattern of organochlorine pesticides (OCPs) through rice crop from selected districts of Punjab Province, Pakistan.

PubMed

Mumtaz, Mehvish; Qadir, Abdul; Mahmood, Adeel; Mehmood, Andleeb; Malik, Riffat Naseem; Li, Jun; Yousaf, Zubaida; Jamil, Nadia; Shaikh, Irfan Ahmed; Ali, Habib; Zhang, Gan

2015-04-01

To evaluate the screening level risk assessment of OCPs in rice (Oryza sativa L.) straw (n=20) and rice grains (n=20), samples were collected from different districts of Punjab Province, Pakistan. ∑OCPs' levels (ng g(-1)) in rice straw and grains ranged from 3.63 to 39.40, 2.72 to 49.89, respectively. DDTs were found predominant over the other detected OCP isomers followed by HCH and heptachlor. Results of one way ANOVA reflected no significant difference for OCPs' levels among sampling sites, except heptachlor for rice grains. ∑OCPs' concentration in rice straw samples was exceeding the minimal residual levels (MRLs) (Australian and Japanese). Results of dietary intake and risk assessment suggested that rice straw is not safe for animals to consume as fodder. Human health was suggested to have some carcinogenic risks by consumption of rice grains, however, no considerable hazardous risk (non-carcinogenic) to human health was found. Copyright © 2014 Elsevier B.V. All rights reserved.

Mapping of Rice Varieties and Sowing Date Using X-Band SAR Data

PubMed Central

Le Toan, Thuy; Nguyen, Lam Dao; Pham Duy, Tien

2018-01-01

Rice is a major staple food for nearly half of the world’s population and has a considerable contribution to the global agricultural economy. While spaceborne Synthetic Aperture Radar (SAR) data have proved to have great potential to provide rice cultivation area, few studies have been performed to provide practical information that meets the user requirements. In rice growing regions where the inter-field crop calendar is not uniform such as in the Mekong Delta in Vietnam, knowledge of the start of season on a field basis, along with the planted rice varieties, is very important for correct field management (timing of irrigation, fertilization, chemical treatment, harvest), and for market assessment of the rice production. The objective of this study is to develop methods using SAR data to retrieve in addition to the rice grown area, the sowing date, and the distinction between long and short cycle varieties. This study makes use of X-band SAR data from COSMO-SkyMed acquired from 19 August to 23 November 2013 covering the Chau Thanh and Thoai Son districts in An Giang province, Viet Nam, characterized by a complex cropping pattern. The SAR data have been analyzed as a function of rice parameters, and the temporal and polarization behaviors of the radar backscatter of different rice varieties have been interpreted physically. New backscatter indicators for the detection of rice paddy area, the estimation of the sowing date, and the mapping of the short cycle and long cycle rice varieties have been developed and assessed. Good accuracy has been found with 92% in rice grown area, 96% on rice long or short cycle, and a root mean square error of 4.3 days in sowing date. The results have been discussed regarding the generality of the methods with respect to the rice cultural practices and the SAR data characteristics. PMID:29361776

Rice Yield and the Fate of Fertilizer Nitrogen as Affected by Addition of Earthworm Casts Collected from Oilseed Rape Fields: A Pot Experiment.

PubMed

Huang, Min; Zhou, Xuefeng; Xie, Xiaobing; Zhao, Chunrong; Chen, Jiana; Cao, Fangbo; Zou, Yingbin

2016-01-01

The mechanism associated with improvement of soil nutritional status by oilseed rape crop, leading to better performance of rice crop, in rice-oilseed rape cropping systems is little known. The present study was aimed to test the hypothesis that earthworm casts produced during oilseed rape-growing season have positive effects on grain yield and fertilizer nitrogen (N) utilization in the subsequent flooded rice crop. A 15N-tracing pot experiment was conducted to determine the effects of earthworm casts collected from oilseed rape fields on yield attributes in rice and the fate of fertilizer N. Soil treated with earthworm casts (soil: earthworm casts = 4: 1, w/w) (EC1) produced 39% higher grain yield than soil only (EC0). EC1 had 18% more panicle number and 10% higher spikelet filling percentage than EC0. Aboveground biomass and harvest index were higher in EC1 than in EC0 by 20% and 15%, respectively. SPAD values in flag leaves were 10% and 22% higher under EC1 than EC0 at 15 and 20 days after heading, respectively. EC1 had 19% higher total N uptake and 18% higher physiological N-use efficiency than EC0. These positive effects of earthworm casts on yield attributes offset negative effects of decreasing N rate from 0.74 g pot-1 (equivalent to the recommended field rate of 150 kg ha-1) to 0.44 g pot-1 (equivalent to 60% of the recommended rate). Fertilizer N retention rate was 7% higher while fertilizer N loss rate was 6% lower in EC1 than in EC0. Our study suggests that earthworm casts produced during oilseed rape-growing season are expected to have the following benefits on the subsequent flooded rice system: (1) improving growth and physiological processes in rice plants and consequently increasing rice grain yield, and (2) increasing fertilizer N retention rate and hence decreasing fertilizer N loss rate and reducing environmental risk.

Rice Yield and the Fate of Fertilizer Nitrogen as Affected by Addition of Earthworm Casts Collected from Oilseed Rape Fields: A Pot Experiment

PubMed Central

Huang, Min; Zhou, Xuefeng; Xie, Xiaobing; Zhao, Chunrong; Chen, Jiana; Cao, Fangbo; Zou, Yingbin

2016-01-01

The mechanism associated with improvement of soil nutritional status by oilseed rape crop, leading to better performance of rice crop, in rice-oilseed rape cropping systems is little known. The present study was aimed to test the hypothesis that earthworm casts produced during oilseed rape-growing season have positive effects on grain yield and fertilizer nitrogen (N) utilization in the subsequent flooded rice crop. A 15N-tracing pot experiment was conducted to determine the effects of earthworm casts collected from oilseed rape fields on yield attributes in rice and the fate of fertilizer N. Soil treated with earthworm casts (soil: earthworm casts = 4: 1, w/w) (EC1) produced 39% higher grain yield than soil only (EC0). EC1 had 18% more panicle number and 10% higher spikelet filling percentage than EC0. Aboveground biomass and harvest index were higher in EC1 than in EC0 by 20% and 15%, respectively. SPAD values in flag leaves were 10% and 22% higher under EC1 than EC0 at 15 and 20 days after heading, respectively. EC1 had 19% higher total N uptake and 18% higher physiological N-use efficiency than EC0. These positive effects of earthworm casts on yield attributes offset negative effects of decreasing N rate from 0.74 g pot–1 (equivalent to the recommended field rate of 150 kg ha–1) to 0.44 g pot–1 (equivalent to 60% of the recommended rate). Fertilizer N retention rate was 7% higher while fertilizer N loss rate was 6% lower in EC1 than in EC0. Our study suggests that earthworm casts produced during oilseed rape-growing season are expected to have the following benefits on the subsequent flooded rice system: (1) improving growth and physiological processes in rice plants and consequently increasing rice grain yield, and (2) increasing fertilizer N retention rate and hence decreasing fertilizer N loss rate and reducing environmental risk. PMID:27880837

Rice Annotation Project Database (RAP-DB): an integrative and interactive database for rice genomics.

PubMed

Sakai, Hiroaki; Lee, Sung Shin; Tanaka, Tsuyoshi; Numa, Hisataka; Kim, Jungsok; Kawahara, Yoshihiro; Wakimoto, Hironobu; Yang, Ching-chia; Iwamoto, Masao; Abe, Takashi; Yamada, Yuko; Muto, Akira; Inokuchi, Hachiro; Ikemura, Toshimichi; Matsumoto, Takashi; Sasaki, Takuji; Itoh, Takeshi

2013-02-01

The Rice Annotation Project Database (RAP-DB, http://rapdb.dna.affrc.go.jp/) has been providing a comprehensive set of gene annotations for the genome sequence of rice, Oryza sativa (japonica group) cv. Nipponbare. Since the first release in 2005, RAP-DB has been updated several times along with the genome assembly updates. Here, we present our newest RAP-DB based on the latest genome assembly, Os-Nipponbare-Reference-IRGSP-1.0 (IRGSP-1.0), which was released in 2011. We detected 37,869 loci by mapping transcript and protein sequences of 150 monocot species. To provide plant researchers with highly reliable and up to date rice gene annotations, we have been incorporating literature-based manually curated data, and 1,626 loci currently incorporate literature-based annotation data, including commonly used gene names or gene symbols. Transcriptional activities are shown at the nucleotide level by mapping RNA-Seq reads derived from 27 samples. We also mapped the Illumina reads of a Japanese leading japonica cultivar, Koshihikari, and a Chinese indica cultivar, Guangluai-4, to the genome and show alignments together with the single nucleotide polymorphisms (SNPs) and gene functional annotations through a newly developed browser, Short-Read Assembly Browser (S-RAB). We have developed two satellite databases, Plant Gene Family Database (PGFD) and Integrative Database of Cereal Gene Phylogeny (IDCGP), which display gene family and homologous gene relationships among diverse plant species. RAP-DB and the satellite databases offer simple and user-friendly web interfaces, enabling plant and genome researchers to access the data easily and facilitating a broad range of plant research topics.

Genetic, chemical, and field management strategies for reducing accumulation of arsenic in rice grains

USDA-ARS?s Scientific Manuscript database

There is public concern over amounts of arsenic contained in rice grains and foods. The World Health Organization (WHO) has set a CODEX limit of 0.2 ppm inorganic arsenic (iAs) in milled white rice, and a lower limit of 0.1 ppm for baby food products. Arsenic is of greater concern in rice than oth...

Monitoring of selected pesticides residue levels in water samples of paddy fields and removal of cypermethrin and chlorpyrifos residues from water using rice bran.

PubMed

Bhattacharjee, Shubhra; Fakhruddin, A N M; Chowdhury, M A Z; Rahman, M A; Alam, M K

2012-08-01

Consumption of pesticides associated foods increased in recent decades in Bangladesh. Most of the pesticides come from paddy, as rice is the main food items here and about 70 % pesticides are used only on paddy fields. Water samples of paddy fields and Kaliganga River of Manikganj district were analyzed to provide base line data on cypermethrin, chlorpyrifos and diazinon residue by using high performance liquid chromatography. Levels of Cypermethrin, chlorpyrifos and diazinon detected in the paddy field water samples were (0.605 ± 0.011 μg/L), (0.06 ± 0.001 μg/L) and (0.039 ± 0.002 μg/L), respectively. 0.11 ± 0.003 μg/L of cypermethrin and 0.012 ± 0.0006 μg/L of chlorpyrifos were also identified in the water samples of Kaligonga River. Diazinon residue was not detected in the river water samples. The detected concentrations of pesticide residues in the river water were below the accepted maximum residue limit (MRL) value of drinking water (0.1 μg/l) adopted by the FAO/WHO Codex Alimentarius Commission. Cypermethrin and chlorpyrifos were chosen for decontamination through rice bran, as it was found in river water. Two gm rice bran could easily decontaminated 95.6 % and 96.4 % of cypermethrin and chlorpyrifos. The result of this study showed that pesticide residue was detected in water samples were below the MRLs value, which can easily be decontaminated through absorption of rice bran.

Evaluation of allelopathic potential and quantification of momilactone A,B from rice hull extracts and assessment of inhibitory bioactivity on paddy field weeds.

PubMed

Chung, Ill Min; Kim, Jung Tae; Kim, Seung-Hyun

2006-04-05

Rice (Oryza sativa L.) hull extracts were used in a bioassay to evaluate the allelopathic potential of rice on the germination and growth of barnyard grass (Echinochloa crus-galli P. Beauv. var. oryzicola Ohwi), to quantify momilactone A and B levels in rice hull germplasm and to assess the inhibitory bioactivity of momilactone A and B as a potential natural source of herbicide for weed control in paddy fields. Four varieties of weeds including E. crus-galli P. Beauv. var. oryzicola Ohwi, Monochoria vaginalis var. plantaginea, Scirpus juncoides, and Eleocharis kuroguwai were tested in the paddy field. Of 99 rice varieties, the top five including Noindari exhibited inhibition effects greater than 50% in average inhibition of dry weight (AIDW). Noindari among them exerted the strongest effect (55.6%). The next five in the ranking exhibited inhibition effects of greater than 40%. Also, 46 varieties had inhibition effects between 20 and 40%, and 29 varieties had inhibition effects greater than 10%. Fourteen varieties had very low inhibitory effects (less than 10%), the lowest of which was Heunbe (4.7%). These varieties showed a mean inhibition of 19.8% for germination rate (GR), 9.9% for germination percentage (GP), 16.6% for leaf dry weight (LDW), 38.9% for straw dry weight (SDW), and 26.8% for root dry weight (RDW). Rice varieties were classified into six categories based on their total momilactones (TMs) (momilactone A + momilactone B). The highest level of momilactone A was found in the Baekna rice variety (34.7 microg g(-1)), and Baekgwangok contained the highest level of momilactone B (37.8 microg g(-1)). In allelopathic potential with genetic properties and morphological characteristics, the total inhibition rate (TIR) was 18.3% for Korean rice varieties, 19.0% for middle maturing varieties, 17.8% for colorless hull varieties, 18.3% for awn varieties, and 19.0% for colorless awn varieties. In addition, Korean varieties showed higher TMs (4.5 microg g(-1)) as

Conserving waste rice for wintering waterfowl in the Mississippi Alluvial Valley

USGS Publications Warehouse

Kross, J.P.; Kaminski, R.M.; Reinecke, K.J.; Pearse, A.T.

2008-01-01

Rice lost before or during harvest operations (hereafter waste rice) provides important food for waterfowl in the Mississippi Alluvial Valley, USA, but >70% of waste rice is lost during autumn. We conducted experiments in 19 production rice fields in Arkansas and Mississippi during autumns 2003 and 2004 to evaluate the ability of common postharvest practices (i.e., burn, mow, roll, disk, or standing stubble) to conserve waste rice. We detected a postharvest treatment effect and a positive effect of initial abundance of waste rice on late-autumn abundance of waste rice (P < 0.022). Standing stubble contained the greatest abundance of waste rice followed by burned, mowed, rolled, and disked stubble. We recommend standing stubble or burning to maximize waste rice abundance for wintering waterfowl.

Conserving waste rice for wintering waterfowl in the Mississippi Alluvial Valley

USGS Publications Warehouse

Kross, J.P.; Kaminski, R.M.; Reinecke, K.J.; Pearse, A.T.

2008-01-01

Rice lost before or during harvest operations (hereafter waste rice) provides important food for waterfowl in the Mississippi Alluvial Valley, USA, but >70% of waste rice is lost during autumn. We conducted experiments in 19 production rice fields in Arkansas and Mississippi during autumns 2003 and 2004 to evaluate the ability of common postharvest practices (i.e., burn, mow, roll, disk, or standing stubble) to conserve waste rice. We detected a postharvest treatment effect and a positive effect of initial abundance of waste rice on late-autumn abundance of waste rice (P ??? 0.022). Standing stubble contained the greatest abundance of waste rice followed by burned, mowed, rolled, and disked stubble. We recommend standing stubble or burning to maximize waste rice abundance for wintering waterfowl.

Fine definition of the pedigree haplotypes of closely related rice cultivars by means of genome-wide discovery of single-nucleotide polymorphisms.

PubMed

Yamamoto, Toshio; Nagasaki, Hideki; Yonemaru, Jun-ichi; Ebana, Kaworu; Nakajima, Maiko; Shibaya, Taeko; Yano, Masahiro

2010-04-27

To create useful gene combinations in crop breeding, it is necessary to clarify the dynamics of the genome composition created by breeding practices. A large quantity of single-nucleotide polymorphism (SNP) data is required to permit discrimination of chromosome segments among modern cultivars, which are genetically related. Here, we used a high-throughput sequencer to conduct whole-genome sequencing of an elite Japanese rice cultivar, Koshihikari, which is closely related to Nipponbare, whose genome sequencing has been completed. Then we designed a high-throughput typing array based on the SNP information by comparison of the two sequences. Finally, we applied this array to analyze historical representative rice cultivars to understand the dynamics of their genome composition. The total 5.89-Gb sequence for Koshihikari, equivalent to 15.7 x the entire rice genome, was mapped using the Pseudomolecules 4.0 database for Nipponbare. The resultant Koshihikari genome sequence corresponded to 80.1% of the Nipponbare sequence and led to the identification of 67,051 SNPs. A high-throughput typing array consisting of 1917 SNP sites distributed throughout the genome was designed to genotype 151 representative Japanese cultivars that have been grown during the past 150 years. We could identify the ancestral origin of the pedigree haplotypes in 60.9% of the Koshihikari genome and 18 consensus haplotype blocks which are inherited from traditional landraces to current improved varieties. Moreover, it was predicted that modern breeding practices have generally decreased genetic diversity Detection of genome-wide SNPs by both high-throughput sequencer and typing array made it possible to evaluate genomic composition of genetically related rice varieties. With the aid of their pedigree information, we clarified the dynamics of chromosome recombination during the historical rice breeding process. We also found several genomic regions decreasing genetic diversity which might be

Multiple abiotic stimuli are integrated in the regulation of rice gene expression under field conditions

PubMed Central

Plessis, Anne; Hafemeister, Christoph; Wilkins, Olivia; Gonzaga, Zennia Jean; Meyer, Rachel Sarah; Pires, Inês; Müller, Christian; Septiningsih, Endang M; Bonneau, Richard; Purugganan, Michael

2015-01-01

Plants rely on transcriptional dynamics to respond to multiple climatic fluctuations and contexts in nature. We analyzed the genome-wide gene expression patterns of rice (Oryza sativa) growing in rainfed and irrigated fields during two distinct tropical seasons and determined simple linear models that relate transcriptomic variation to climatic fluctuations. These models combine multiple environmental parameters to account for patterns of expression in the field of co-expressed gene clusters. We examined the similarities of our environmental models between tropical and temperate field conditions, using previously published data. We found that field type and macroclimate had broad impacts on transcriptional responses to environmental fluctuations, especially for genes involved in photosynthesis and development. Nevertheless, variation in solar radiation and temperature at the timescale of hours had reproducible effects across environmental contexts. These results provide a basis for broad-based predictive modeling of plant gene expression in the field. DOI: http://dx.doi.org/10.7554/eLife.08411.001 PMID:26609814

Rice Crop Monitoring Using Microwave and Optical Remotely Sensed Image Data

NASA Astrophysics Data System (ADS)

Suga, Y.; Konishi, T.; Takeuchi, S.; Kitano, Y.; Ito, S.

Hiroshima Institute of Technology HIT is operating the direct down-links of microwave and optical satellite data in Japan This study focuses on the validation for rice crop monitoring using microwave and optical remotely sensed image data acquired by satellites referring to ground truth data such as height of crop ratio of crop vegetation cover and leaf area index in the test sites of Japan ENVISAT-1 ASAR data has a capability to capture regularly and to monitor during the rice growing cycle by alternating cross polarization mode images However ASAR data is influenced by several parameters such as landcover structure direction and alignment of rice crop fields in the test sites In this study the validation was carried out combined with microwave and optical satellite image data and ground truth data regarding rice crop fields to investigate the above parameters Multi-temporal multi-direction descending and ascending and multi-angle ASAR alternating cross polarization mode images were used to investigate rice crop growing cycle LANDSAT data were used to detect landcover structure direction and alignment of rice crop fields corresponding to the backscatter of ASAR As the result of this study it was indicated that rice crop growth can be precisely monitored using multiple remotely sensed data and ground truth data considering with spatial spectral temporal and radiometric resolutions

Persistence behavior of metamifop and its metabolite in rice ecosystem.

PubMed

Barik, Suhrid Ranjan; Ganguly, Pritam; Patra, Sandip; Dutta, Swaraj Kumar; Goon, Arnab; Bhattacharyya, Anjan

2018-02-01

A field experiment was conducted to determine the persistence of metamifop in transplanted rice crop for two seasons. Metamifop 10% EC was applied at two doses: 100 g a.i. ha -1 and 200 g a.i. ha -1 at 2-3 leaf stage of Echinochloa crusgalli. The residues of metamifop along with its major metabolite, N-(2-fluorophenyl)-2-hydroxy-N-methylpropionamide (HFMPA), were estimated in rice plant, field water and soil using Liquid Chromatography Mass Spectrometry. Limit of detection and limit of quantification of the method for both the compounds were set at 0.003 μg g -1 and 0.010 μg g -1 respectively. Metamifop showed less persistence in field water and rice plant as compared to soil samples. Presence of HFMPA was recorded in rice plant and soil. Both the compounds were found below level of quantification in harvest samples of straw, grains, husk and soil. A safe waiting period of 52 d was suggested for harvesting of rice when metamifop was applied at 100 g a.i. ha -1 (recommended dose). Copyright © 2017 Elsevier Ltd. All rights reserved.

The Impact of Herbicide-Resistant Rice Technology on Phenotypic Diversity and Population Structure of United States Weedy Rice1[W][OPEN

PubMed Central

Burgos, Nilda Roma; Singh, Vijay; Tseng, Te Ming; Black, Howard; Young, Nelson D.; Huang, Zhongyun; Hyma, Katie E.; Gealy, David R.; Caicedo, Ana L.

2014-01-01

The use of herbicide-resistant (HR) Clearfield rice (Oryza sativa) to control weedy rice has increased in the past 12 years to constitute about 60% of rice acreage in Arkansas, where most U.S. rice is grown. To assess the impact of HR cultivated rice on the herbicide resistance and population structure of weedy rice, weedy samples were collected from commercial fields with a history of Clearfield rice. Panicles from each weedy type were harvested and tested for resistance to imazethapyr. The majority of plants sampled had at least 20% resistant offspring. These resistant weeds were 97 to 199 cm tall and initiated flowering from 78 to 128 d, generally later than recorded for accessions collected prior to the widespread use of Clearfield rice (i.e. historical accessions). Whereas the majority (70%) of historical accessions had straw-colored hulls, only 30% of contemporary HR weedy rice had straw-colored hulls. Analysis of genotyping-by-sequencing data showed that HR weeds were not genetically structured according to hull color, whereas historical weedy rice was separated into straw-hull and black-hull populations. A significant portion of the local rice crop genome was introgressed into HR weedy rice, which was rare in historical weedy accessions. Admixture analyses showed that HR weeds tend to possess crop haplotypes in the portion of chromosome 2 containing the ACETOLACTATE SYNTHASE gene, which confers herbicide resistance to Clearfield rice. Thus, U.S. HR weedy rice is a distinct population relative to historical weedy rice and shows modifications in morphology and phenology that are relevant to weed management. PMID:25122473

Genomic patterns of nucleotide diversity in divergent populations of U.S. weedy rice

USDA-ARS?s Scientific Manuscript database

Weedy rice is a significant problem in cultivated rice fields throughout the world, and is an emerging threat in regions where it was previously absent. Prior research has classified weedy rice as the same species as Asian cultivated rice (Oryza sativa L.). This close genetic relationship makes cont...

Effects of Insect-Proof Net Cultivation, Rice-Duck Farming, and Organic Matter Return on Rice Dry Matter Accumulation and Nitrogen Utilization

PubMed Central

Liu, Xin; Xu, Guochun; Wang, Qiangsheng; Hang, Yuhao

2017-01-01

Insect-proof net cultivation (IPN), rice-duck farming (RD), and organic matter return (OM) are important methods to realize sustainable development of rice production. A split-plot field experiment was performed to study the effects of IPN, RD, and OM on the rice yield, dry matter accumulation and N utilization. Results showed that compared to inorganic N fertilizer (IN), wheat straw return, and biogas residue return increased the rice yield by 2.11–4.28 and 4.78–7.67%, respectively, and also improved dry matter and N accumulation after the elongation stage (EG), dry matter and N translocation, and N recovery efficiency (NRE). These results attributed to an increase in leaf SPAD values and net photosynthetic rate (Pn) after the EG. Compared to conventional rice farming (CR), RD promoted the rice yield by 1.52–3.74%, and contributed to higher the leaf photosynthesis, dry matter and N accumulation, dry matter and N translocation, and NRE. IPN decreased the intensity of sun radiation in the nets due to the coverage of the insect-proof nets, which declined the leaf Pn, dry matter accumulation and translocation, N absorption and translocation, and NRE compared to open field cultivation (OFC). The rice yield of IPN were 2.48–4.98% lower than that of OFC. Compared to the interaction between CR and IN, the interaction between RD and OM improved the rice yield by 5.26–9.33%, and increased dry matter and N accumulation after the EG, dry matter and N translocation, and NRE. These results indicated that OM, RD and the interaction between RD and OM could promote dry matter accumulation and N utilization, which was beneficial to improve the rice yield. PMID:28174589

Single and combination insecticides evaluated as regulatory immersion treatments to eliminate third-instar Japanese beetle (Coleoptera: Scarabaeidae) from small diameter field-grown and containerized nursery plants

USDA-ARS?s Scientific Manuscript database

Japanese beetles, Popillia japonica Newman, are a nursery regulatory pest. Immersion of field-grown plants harvested as balled and burlapped (B&B) or container plants grown in pine bark substrates in a solution of chlorpyrifos or bifenthrin is allowed for certification in the Domestic Japanese Beet...

Genome Wide Association Mapping of Grain Arsenic, Copper, Molybdenum and Zinc in Rice (Oryza sativa L.) Grown at Four International Field Sites

PubMed Central

Norton, Gareth J.; Douglas, Alex; Lahner, Brett; Yakubova, Elena; Guerinot, Mary Lou; Pinson, Shannon R. M.; Tarpley, Lee; Eizenga, Georgia C.; McGrath, Steve P.; Zhao, Fang-Jie; Islam, M. Rafiqul; Islam, Shofiqul; Duan, Guilan; Zhu, Yongguan; Salt, David E.; Meharg, Andrew A.; Price, Adam H.

2014-01-01

The mineral concentrations in cereals are important for human health, especially for individuals who consume a cereal subsistence diet. A number of elements, such as zinc, are required within the diet, while some elements are toxic to humans, for example arsenic. In this study we carry out genome-wide association (GWA) mapping of grain concentrations of arsenic, copper, molybdenum and zinc in brown rice using an established rice diversity panel of ∼300 accessions and 36.9 k single nucleotide polymorphisms (SNPs). The study was performed across five environments: one field site in Bangladesh, one in China and two in the US, with one of the US sites repeated over two years. GWA mapping on the whole dataset and on separate subpopulations of rice revealed a large number of loci significantly associated with variation in grain arsenic, copper, molybdenum and zinc. Seventeen of these loci were detected in data obtained from grain cultivated in more than one field location, and six co-localise with previously identified quantitative trait loci. Additionally, a number of candidate genes for the uptake or transport of these elements were located near significantly associated SNPs (within 200 kb, the estimated global linkage disequilibrium previously employed in this rice panel). This analysis highlights a number of genomic regions and candidate genes for further analysis as well as the challenges faced when mapping environmentally-variable traits in a highly genetically structured diversity panel. PMID:24586963

Field evaluation of resistance of transgenic rice containing a synthetic cry1Ab gene from Bacillus thuringiensis Berliner to two stem borers.

PubMed

Ye, G Y; Shu, Q Y; Yao, H W; Cui, H R; Cheng, X Y; Hu, C; Xia, Y W; Gao, M W; Altosaar, I

2001-02-01

Two transgenic rice (Oryza sativa L.) lines, KMD1 and KMD2 at the R4 generation, transformed with a synthetic cry1Ab gene from Bacillus thuringiensis Berliner, were first evaluated for stem borer resistance in the field during the rice growing season of 1998 in two areas of Zhejiang Province, China. Both KMD1 and KMD2 were highly resistant to the stem borers Chilo suppressalis (Walker) and Scirpophaga incertulas (Walker), and were completely undamaged during the whole rice growing season. In contrast, damage to the plants of the untransformed parental control (Xiushui 11) was in the form of deadhearts or whiteheads. Under natural infestation by the C. suppressalis, the damage to control plants reached a peak of 88.7% of plants and 20.1% of tillers encountered with deadhearts. Under artificial and natural infestation of neonate striped stem borers at the vegetative stage and booting stage, 100% of plants and 25.6% of tillers, 78.9% of plants and 15.6% of productive tillers among artificially infested control plants were observed with the symptom of deadhearts and whiteheads, respectively. Damage to the control plants from artificial infestation by the S. incertulas reached a peak of 97.0% of plants and 22.9% of tillers damaged. The field research indicated that both KMD1 and KMD2 show great potential for protecting rice from attack by these two stem borers.

Breeding patterns of the JE vector Culex gelidus and its insect predators in rice cultivation areas of northern peninsular Malaysia.

PubMed

Abu Hassan, A; Hamady, D; Tomomitsu, S; Michael, B; Jameel S L, A S

2010-12-01

Japanese encephalitis (JE) virus activity is an important cause of viral encephalitis in Southeast Asia. In Malaysia, JEV activity has been first detected in Culex gelidus in 1976. Since then, no study has fully addressed the seasonal dynamics of this mosquito. As irrigated rice production expands, the incidence of JEV vectors, particularly Cx. gelidus is expected to increase. We surveyed Penang Island to determine the breeding patterns of Cx. gelidus and their potential insect predators, in relation to habitat/niche and rice growing period. Six rice fields proper (RFP) and related drainage canals (DC) were visited through three cultivation cycles (CCs) over 17 months. Weekly visits were performed to each of the 36 sites and mosquito larvae and aquatic insects were sampled from RFP and DCs using dippers. Culex gelidus was abundant in RFP and almost absent in DCs. Its densities usually were high during the first and 3rd CC and when the RFs were in Fp, Pp and Gp. In DCs, the mosquito was abundant during Mp, e.g., 2nd CC. Predators, especially those belonging to the families Corixidae, Coenagrionidae and Dytiscidae, were more present in RFP. Predator numbers usually were high during the first CC; in some cases predator abundance peaked during other CCs, e.g., corixids and dysticids. In RFP, neither corixids nor coenagrionids showed any positive correlation with densities of Cx. gelidus. However, dytiscids' population peaked when the mosquito densities were on the rise. These observations suggest that Cx. gelidus is active during the period of rice cultivation. Operational vector control through bio-control or with insecticides near the end of the rice cultivation season in RFP may prove beneficial in reducing the density of Cx. gelidus, but also the amount of bio-agent or insecticide applied on riceland.

An Inclusive Investigation on Conceivable Performance of Rice Straw Incinerated Electricity Generation

NASA Astrophysics Data System (ADS)

Bhattacharjee, Subhadeep; Mohanta, Subhajit

2018-06-01

Biomass energy is one of the potential renewable energy sources which occupy 77% of the available natural resources of the world. In India, agro residues constitute a major part of the total annual production of the biomass resource. Rice is the major crop in India that leaves substantial quantity of straw in the field. 34% of rice straw residue produced in the country is surplus and is either left in the field as uncollected or to a large extent open-field burnt. Thus, the unutilized rice straw is found promising for heat and power generation either through incineration (direct combustion) or thermo chemical conversion. This present work envisages the comprehensive performative evaluation of a rice straw supported biomass incineration power plant mainly through plant performance characterization, plant economics, and co-firing issues with emission analysis.

An Inclusive Investigation on Conceivable Performance of Rice Straw Incinerated Electricity Generation

NASA Astrophysics Data System (ADS)

Bhattacharjee, Subhadeep; Mohanta, Subhajit

2018-03-01

Biomass energy is one of the potential renewable energy sources which occupy 77% of the available natural resources of the world. In India, agro residues constitute a major part of the total annual production of the biomass resource. Rice is the major crop in India that leaves substantial quantity of straw in the field. 34% of rice straw residue produced in the country is surplus and is either left in the field as uncollected or to a large extent open-field burnt. Thus, the unutilized rice straw is found promising for heat and power generation either through incineration (direct combustion) or thermo chemical conversion. This present work envisages the comprehensive performative evaluation of a rice straw supported biomass incineration power plant mainly through plant performance characterization, plant economics, and co-firing issues with emission analysis.

Mercury in rice (Oryza sativa L.) and rice-paddy soils under long-term fertilizer and organic amendment.

PubMed

Tang, Zhenya; Fan, Fangling; Wang, Xinyue; Shi, Xiaojun; Deng, Shiping; Wang, Dingyong

2018-04-15

High levels of mercury (Hg), especially methylmercury (MeHg), in rice is of concern due to its potential of entering food chain and the high toxicity to human. The level and form of Hg in rice could be influenced by fertilizers and other soil amendments. Studies were conducted to evaluate the effect of 24 years application of chemical fertilizers and organic amendments on total Hg (THg) and MeHg and their translocation in soil, plants, and rice grain. All treatments led to significantly higher concentrations of MeHg in grain than those from the untreated control. Of nine treatments tested, chemical fertilizers combining with returning rice straw (NPK1+S) led to highest MeHg concentration in grain and soil; while the nitrogen and potassium (NK) treatment led to significantly higher THg in grain. Concentrations of soil MeHg were significantly correlated with THg in soil (r = 0.59 *** ) and MeHg in grain (r = 0.48 *** ). Calcium superphosphate negatively affected plant bioavailability of soil Hg. MeHg concentration in rice was heavily influenced by soil Hg levels. Phosphorus fertilizer was a main source contributing to soil THg, while returning rice straw to the field contributed significantly to MeHg in soil and rice grain. As a result, caution should be exercised in soil treatment or when utilizing Hg-contaminated soils to produce rice for human consumption. Strategic management of rice straw and phosphorus fertilizer could be effective strategies of lowering soil Hg, which would ultimately lower MeHg in rice and the risk of Hg entering food chain. Copyright © 2017 Elsevier Inc. All rights reserved.

Outbreak of Tagosodes orizicolus (Muir) in Texas rice

USDA-ARS?s Scientific Manuscript database

The rice planthopper, Tagosodes orizicolus, is reported for the first time in Texas, where it is superabundant in rice fields in four counties (Brazoria, Colorado, Harris, and Wharton). The species is a known vector of the viral disease hoja blanca, which can reduce yields up to 50%, and hopper burn...

Occurrence and distribution study of residues from pesticides applied under controlled conditions in the field during rice processing.

PubMed

Pareja, Lucía; Colazzo, Marcos; Pérez-Parada, Andrés; Besil, Natalia; Heinzen, Horacio; Böcking, Bernardo; Cesio, Verónica; Fernández-Alba, Amadeo R

2012-05-09

The results of an experiment to study the occurrence and distribution of pesticide residues during rice cropping and processing are reported. Four herbicides, nine fungicides, and two insecticides (azoxystrobin, byspiribac-sodium, carbendazim, clomazone, difenoconazole, epoxiconazole, isoprothiolane, kresoxim-methyl, propanil, quinclorac, tebuconazole, thiamethoxam, tricyclazole, trifloxystrobin, λ-cyhalotrin) were applied to an isolated rice-crop plot under controlled conditions, during the 2009-2010 cropping season in Uruguay. Paddy rice was harvested and industrially processed to brown rice, white rice, and rice bran, which were analyzed for pesticide residues using the original QuEChERS methodology and its citrate variation by LC-MS/MS and GC-MS. The distribution of pesticide residues was uneven among the different matrices. Ten different pesticide residues were found in paddy rice, seven in brown rice, and eight in rice bran. The highest concentrations were detected in paddy rice. These results provide information regarding the fate of pesticides in the rice food chain and its safety for consumers.

Alternate wetting and drying decreases methylmercury in flooded rice (Oryza sativa) systems

USGS Publications Warehouse

Tanner, K. Christy; Windham-Myers, Lisamarie; Marvin-DiPasquale, Mark C.; Fleck, Jacob; Linquist, Bruce A.

2018-01-01

In flooded soils, including those found in rice (Oryza sativa L.) fields, microbes convert inorganic Hg to more toxic methylmercury (MeHg). Methylmercury is accumulated in rice grain, potentially affecting health. Methylmercury in rice field surface water can bioaccumulate in wildlife. We evaluated how introducing aerobic periods into an otherwise continuously flooded rice growing season affects MeHg dynamics. Conventional continuously flooded (CF) rice field water management was compared with alternate wetting and drying, where irrigation was stopped twice during the growing season, allowing soil to dry to 35% volumetric moisture content, at which point plots were reflooded (AWD-35). Methylmercury studies began at harvest in Year 3 and throughout Year 4 of a 4-yr replicated field experiment. Bulk soil, water, and plant samples were analyzed for MeHg and total Hg (THg), and iron (Fe) speciation was measured in soil samples. Rice grain yield over 4 yr did not differ between treatments. Soil chemistry responded quickly to AWD-35 dry-downs, showing significant oxidation of Fe(II) accompanied by a significant reduction of MeHg concentration (76% reduction at harvest) compared with CF. Surface water MeHg decreased by 68 and 39% in the growing and fallow seasons, respectively, suggesting that the effects of AWD-35 management can last through to the fallow season. The AWD-35 treatment reduced rice grain MeHg and THg by 60 and 32%, respectively. These results suggest that the more aerobic conditions caused by AWD-35 limited the activity of Hg(II)-methylating microbes and may be an effective way to reduce MeHg concentrations in rice ecosystems.

Water regime-nitrogen fertilizer incorporation interaction: Field study on methane and nitrous oxide emissions from a rice agroecosystem in Harbin, China.

PubMed

Dong, Wenjun; Guo, Jia; Xu, Lijun; Song, Zhifeng; Zhang, Jun; Tang, Ao; Zhang, Xijuan; Leng, Chunxu; Liu, Youhong; Wang, Lianmin; Wang, Lizhi; Yu, Yang; Yang, Zhongliang; Yu, Yilei; Meng, Ying; Lai, Yongcai

2018-02-01

Water regime and nitrogen (N) fertilizer are two important factors impacting greenhouse gases (GHG) emission from paddy field, whereas their effects have not been well studied in cold region. In this study, we conducted a two-year field experiment to study the impacts of water regime and N fertilizer on rice yields and GHG emissions in Harbin, China, a cold region located in high latitudes. Our results showed that intermittent irrigation significantly decreased methane (CH 4 ) emission compared with continuous flooding, however, the decrement was far lower than the global average level. The N 2 O emissions were very small when flooded but peaked at the beginning of the disappearance of floodwater. The N fertilizer treatments increased CH 4 emissions at low level (75kgN/ha). But both CH 4 and N 2 O emissions were uninfluenced at the levels of 150kgN/ha and 225kgN/ha. Rice yields increased under intermittent irrigation and were highest at the level of 150kgN/ha. From our results, we recommended that the intermittent irrigation and 150kgN/ha as the ideal water regime-nitrogen fertilizer incorporation for this area to achieve low GHG emissions without impacting rice yields. Copyright © 2017. Published by Elsevier B.V.

Biofortified indica rice attains iron and zinc nutrition dietary targets in the field

USDA-ARS?s Scientific Manuscript database

Iron (Fe) and zinc (Zn) deficiencies are the most prevalent micronutrient malnutrition globally1. Fe in rice has proven efficacious in improving serum ferritin concentration and body Fe levels2. Rapid progress in biofortification demonstrates the feasibility to enhance Fe in polished rice by expre...

Polychlorinated dibenzo-p-dioxins, dibenzofurans, and dioxin-like polychlorinated biphenyls in rice straw smoke and their origins in Japan.

PubMed

Minomo, Kotaro; Ohtsuka, Nobutoshi; Nojiri, Kiyoshi; Hosono, Shigeo; Kawamura, Kiyoshi

2011-08-01

Polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs), and dioxin-like polychlorinated biphenyls (DL-PCBs) contained in the smoke generated from rice straw burning in post-harvest paddy fields in Japan were analyzed to determine their congener profiles. Both the apportionment of toxic equivalent (TEQ) by using indicative congeners and the comparison of the homolog profiles showed that the PCDDs/PCDFs/DL-PCBs present in the rice-straw smoke were greatly influenced by those present as impurities in pentachlorophenol (PCP) and chlornitrofen (CNP, 4-nitrophenyl-2,4,6-trichlorophenyl ether) formulations that had been widely used as herbicides in paddy fields in Japan. Further, in order to investigate the effects of paddy-field soil on the PCDDs/PCDFs/DL-PCBs present in rice-straw smoke, PCDD/PCDF/DL-PCB homolog profiles of rice straw, rice-straw smoke and paddy-field soil were compared. Rice-straw smoke was generated by burning rice straw on a stainless-steel tray in a laboratory. The results suggested that the herbicides-originated PCDDs/PCDFs/DL-PCBs and the atmospheric PCDDs/PCDFs/DL-PCBs contributed predominantly to the presence of PCDDs/PCDFs/DL-PCBs in the rice-straw smoke while the contribution of PCDDs/PCDFs/DL-PCBs formed during rice straw burning was relatively minimal. The major sources of the PCDDs/PCDFs/DL-PCBs found in the rice-straw smoke were attributed primarily to the paddy-field soil adhered to the rice straw surface and secondarily to the air taken by the rice straw. The principal component analysis supported these conclusions. It is concluded that rice straw burning at paddy fields acts as a driving force in the transfer of PCDDs/PCDFs/DL-PCBs from paddy-field soil to the atmosphere. Copyright © 2011 Elsevier Ltd. All rights reserved.

A new species of Leptotrombidium (Acari:Trombiculidae) collected in active rice fields in northern Thailand.

PubMed

Tanskul, P; Linthicum, K J

1997-05-01

Leptotrombidium (Leptotrombidium) chiangraiensis Tanskul & Linthicum is described and illustrated as new from specimens collected from the rodents Rattus rattus (L., 1758), Rattus argentiventer (Robinson & Kloss, 1916), Rattus losea (Swinhoe, 1870), and Bandicota indica (Bechstein, 1800) in Chiangrai Province northern Thailand. The new species was collected in active rice fields and adjacent fruit plantation areas. The etiological agent of scrub typhus, Orientia (formerly Rickettsia) tsutsugamushi (Hayashi), has been isolated from patients who live and work in the same habitat where L. chiangraiensis is the predominant Leptotrombidium species.

Antioxidative pyranonigrins in rice mold starters and their suppressive effect on the expression of blood adhesion molecules.

PubMed

Miyake, Yoshiaki; Mochizuki, Mika; Ito, Chihiro; Itoigawa, Masataka; Osawa, Toshihiko

2008-06-01

Antioxidants having a 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capacity in rice mold starters, which are used for the preparation of various Japanese fermented foods, and their effectiveness against the expression of blood adhesion molecules were examined. An antioxidant was isolated from the rice mold starters used for shochu and identified as pyranonigrin-S (PG-S) by (1)H-NMR, (13)C-NMR, and FAB-MS analyses. It was a derivative of pyranonigrin-A (PG-A), which has been isolated as an antioxidant from the rice mold starters. Pyranonigrins PG-A and PG-S were found to exist in spores on rice mold starters which had been prepared by Aspergillus awamori, A. kawachii, and A. saitoi. PG-S exhibited a higher level of DPPH radical scavenging activity than PG-A. PG-A was found to have a significant suppressive effect on the expression of vascular cell adhesion molecule-1 (VCAM-1) in human umbilical vein endothelial cells (HUVECs) induced by tumor necrosis factor-alpha (TNF-alpha) (P<0.05).

Isolation and identification of a lethal rhabdovirus from farmed rice field eels Monopterus albus.

PubMed

Ou, Tong; Zhu, Ruo-Lin; Chen, Zhong-Yuan; Zhang, Qi-Ya

2013-11-06

We provide the first description of a virus responsible for a systemic hemorrhagic disease causing high mortality in farmed rice field eels Monopterus albus in China. Typical signs exhibited by the diseased fish were extensive hemorrhages in the skin and viscera and some neurological signs, such as loss of equilibrium and disorganized swimming. Histopathological examination revealed various degrees of necrosis within the spleen and liver. Virus isolation was attempted from visceral tissues of diseased fish by inoculation on 6 fish cell lines. Typical cytopathic effects (CPE) were produced in bluegill fry (BF2) cells, so this cell line was chosen for further isolation and propagation of the virus. Electron microscopy observation showed that the negative stained viral particles had the characteristic bullet shape of rhabdoviruses and an estimated size of 60 × 120 nm. We therefore tentatively refer to this virus as Monopterus albus rhabdovirus (MoARV). Molecular characterization of MoARV, including sequence analysis of the nucleoprotein (N), phosphoprotein (P), and glycoprotein (G) genes, revealed 94.5 to 97.3% amino acid similarity to that of Siniperca chuatsi rhabdovirus. Phylogenetic analysis based on the amino acid sequences of N and G proteins indicated that MoARV should be a member of the genus Vesiculovirus. Koch's postulates were fulfilled by infecting healthy rice field eels with MoARV, which produced an acute infection. RT-PCR analysis demonstrated that MoARV RNA could be detected in both naturally and experimentally infected fish. The data suggest that MoARV was the causative pathogen of the disease.

Punctual Transcriptional Regulation by the Rice Circadian Clock under Fluctuating Field Conditions[OPEN

PubMed Central

Matsuzaki, Jun; Kawahara, Yoshihiro; Izawa, Takeshi

2015-01-01

Plant circadian clocks that oscillate autonomously with a roughly 24-h period are entrained by fluctuating light and temperature and globally regulate downstream genes in the field. However, it remains unknown how punctual internal time produced by the circadian clock in the field is and how it is affected by environmental fluctuations due to weather or daylength. Using hundreds of samples of field-grown rice (Oryza sativa) leaves, we developed a statistical model for the expression of circadian clock-related genes integrating diurnally entrained circadian clock with phase setting by light, both responses to light and temperature gated by the circadian clock. We show that expression of individual genes was strongly affected by temperature. However, internal time estimated from expression of multiple genes, which may reflect transcriptional regulation of downstream genes, is punctual to 22 min and not affected by weather, daylength, or plant developmental age in the field. We also revealed perturbed progression of internal time under controlled environment or in a mutant of the circadian clock gene GIGANTEA. Thus, we demonstrated that the circadian clock is a regulatory network of multiple genes that retains accurate physical time of day by integrating the perturbations on individual genes under fluctuating environments in the field. PMID:25757473

[Effects of Water and Nitrogenous Fertilizer Coupling on CH4 and N2O Emission from Double-Season Rice Paddy Field].

PubMed

Fu, Zhi-qiang; Long, Pan; Liu, Yi-yi; Zhong, Juan; Long, Wen-fei

2015-09-01

To provide support for the efficient use of water and fertilizer technology to double-season rice cultivation, water and fertilizer coupling mode was applied in this research, including two irrigation methods and four N levels. The irrigation methods were flood irrigation and intermittent irrigation, while four N levels were high-N, middle-N, low-N and none-N. Field experiment was conducted to study the effect of water and fertilizer coupling mode on CH4 and N2O emission. The results showed that the accumulated CH4 emissions were significantly reduced by intermittent irrigation, in comparison with flood irrigation, the reduction in early rice season were from 13. 18 kg.hm-2 to 87. 90 kg.hm-2, and were from 74. 48 kg.hm-2 to 131. 07 kg.hm-2 in late rice season, with a rate of 24. 4% -67. 4% and 42. 5% -65. 5% respectively; whereas the accumulated N20 emissions were increased, the increment were from 0. 03 kg.hm-2 to 0. 24 kg.hm-2 in early rice season and from 0. 35 kg.hm-2 to 1. 53 kg.hm-2 in late rice season when compared flood irrigation, increased by 6.2% -18. 3% and 40.2% - 80.9% respectively. On the whole, intermittent irrigation reduces the warming potential of greenhouse gases (GWP), which were decreased by 18. 8% to 58. 6% in early rice season and by 34. 4% to 60. 1% in late rice season, and the reduction of total GWP were from 2 388 to 4 151 kg. hm-2 (CO2 eq), with a rate of 41% -54% . Through correlation analysis it found that CH4 emissions from soil were significantly related with soil solution Eh and solution CH4 concentration. In comparison with the flood irrigation, the application of intermittent irrigation in double-season rice cultivation was conducive to CH4 reduction, though the increase came in N2O, but the GWPs were significantly reduced. Comprehensively, intermittent irrigation matching with middle-N is more benefit to double-season rice cultivation.

The effect of lactic acid bacterial starter culture and chemical additives on wilted rice straw silage.

PubMed

Wang, Yan-Su; Shi, Wei; Huang, Lin-Ting; Ding, Cheng-Long; Dai, Chuan-Chao

2016-04-01

Lactic acid bacteria (LAB) are suitable for rice straw silage fermentation, but have been studied rarely, and rice straw as raw material for ensiling is difficult because of its disadvantages, such as low nutrition for microbial activities and low abundances of natural populations of LAB. So we investigated the effect of application of LAB and chemical additives on the fermentation quality and microbial community of wilted rice straw silage. Treatment with chemical additives increased the concentrations of crude protein (CP), water soluble carbohydrate (WSC), acetic acid and lactic acid, reduced the concentrations of acid detergent fiber (ADF) and neutral detergent fiber (NDF), but did not effectively inhibit the growth of spoilage organisms. Inoculation with LABs did not improve the nutritional value of the silage because of poor growth of LABs in wilted rice straw. Inoculation with LAB and addition of chemical materials improved the quality of silage similar to the effects of addition of chemical materials alone. Growth of aerobic and facultatively anaerobic bacteria was inhibited by this mixed treatment and the LAB gradually dominated the microbial community. In summary, the fermentation quality of wilted rice straw silage had improved by addition of LAB and chemical materials. © 2015 Japanese Society of Animal Science.

A three-season field study on the in-situ remediation of Cd-contaminated paddy soil using lime, two industrial by-products, and a low-Cd-accumulation rice cultivar.

PubMed

Yan-Bing, He; Dao-You, Huang; Qi-Hong, Zhu; Shuai, Wang; Shou-Long, Liu; Hai-Bo, He; Han-Hua, Zhu; Chao, Xu

2017-02-01

To mitigate the serious problem of Cd-contaminated paddy soil, we investigated the remediation potential of combining in-situ immobilization with a low-Cd-accumulation rice cultivar. A three-season field experiment compared the soil pH, available Cd and absorption of Cd by three rice cultivars with different Cd accumulation abilities grown in Cd-contaminated paddy soil amended with lime (L), slag (S), and bagasse (B) alone or in combination. The three amendments applied alone and in combination significantly increased soil pH, reduced available Cd and absorption of Cd by rice with no effect on grain yield. Among these, the LS and LSB treatments reduced the brown rice Cd content by 38.3-69.1% and 58.3-70.9%, respectively, during the three seasons. Combined with planting of a low-Cd-accumulation rice cultivar (Xiang Zaoxian 32) resulted in a Cd content in brown rice that met the contaminant limit (≤0.2mgkg -1 ). However, the grain yield of the low-Cd-accumulation rice cultivar was approximately 30% lower than the other two rice cultivars. Applying LS or LSB as amendments combined with planting a low-Cd-accumulation rice cultivar is recommended for the remediation of Cd-contaminated paddy soil. The selection and breeding of low-Cd-accumulation rice cultivars with high grain production requires further research. Copyright © 2016 Elsevier Inc. All rights reserved.

Effect of timing of joint application of hydroquinone and dicyandiamide on nitrous oxide emission from irrigated lowland rice paddy field.

PubMed

Li, Xianglan; Zhang, Guangbin; Xu, Hua; Cai, Zucong; Yagi, Kazuyuki

2009-06-01

A field experiment was conducted to study the effect of timing of joint application of urease inhibitor hydroquinone (HQ) and nitrification inhibitor dicyandiamide (DCD) on N(2)O emission from irrigated lowland rice paddy field. Four treatments including Treatment CK (the control with urea alone), HQ/DCD-1 (application of HQ and DCD together with fertilizer before transplanting), HQ/DCD-2 (HQ and DCD with fertilizer at tillering stage) and HQ/DCD-3 (HQ and DCD with fertilizer at panicle initiation stage) were designed and implemented separately during rice growth period. Seasonal peaks of N(2)O flux occurred during midseason drainage and significant negative correlation between N(2)O flux and water layer depth was observed (r=-0.69 to -0.75, P<0.01). Mean N(2)O flux was the highest in the control with urea alone, while joint addition of HQ and DCD with urea lowered mean N(2)O flux considerably (P<0.05). Total N(2)O emission during rice growth season in Treatment CK, HQ/DCD-1, HQ/DCD-2 and HQ/DCD-3 was 3.90, 2.98, 1.73 and 3.23kgN(2)O-N ha(-1), respectively. Application of HQ and DCD together with basal fertilizer, tillering fertilizer and panicle initiation fertilizer decreased the total N(2)O emission by 24%, 56% and 17%, respectively, while increased grain yield by 10%, 18% and 6%, respectively. Effect of application of inhibitors on N(2)O emission during the continuous period from incorporation of HQ and DCD to rice harvest was also studied, where results indicating that the highest inhibiting efficiency of inhibitors on N(2)O emission was recorded when HQ and DCD applied with fertilizer at tillering stage.

Field Phenotyping Strategies and Breeding for Adaptation of Rice to Drought†

PubMed Central

Fischer, Ken S.; Fukai, Shu; Kumar, Arvind; Leung, Hei; Jongdee, Boonrat

2012-01-01

This paper is a section of the book “Drought phenotyping in crops: from theory to practice” (Monneveux Philippe and Ribaut Jean-Marcel eds, published by CGIAR Generation Challenge Programme. Texcoco, Mexico). The section describes recent experience in drought phenotyping in rice which is one of the most drought-susceptible crops. The section contains genetic and genomic resources for drought adaptation and methods for selection of drought-resistant varieties in rice. In appendix, there is experience from Thailand on integration of direct selection for grain yield and physiological traits to confer drought resistance. PMID:22934036

Lead pollution from waterfowl hunting in wetlands and rice fields in Argentina.

PubMed

Romano, Marcelo; Ferreyra, Hebe; Ferreyroa, Gisele; Molina, Fernando V; Caselli, Andrea; Barberis, Ignacio; Beldoménico, Pablo; Uhart, Marcela

2016-03-01

The pollution of wetlands by lead derived from waterfowl hunting with lead shot was investigated. We determined soil pellet density and Pb concentration in soil, water and vegetation in natural wetlands and rice fields in central-eastern Santa Fe province, Argentina. Pellet density varied greatly among hunting sites (between 5.5-141 pellets/m(2)) and pellets were present in some control sites. Soil Pb concentration in most hunting sites (approximately 10-20 mg kg(-1)) was not much higher than in control sites (~5-10 mg kg(-1)), with the exception of the site with highest pellet density, which also had a high Pb soil concentration. In water, on the other hand, Pb concentration was similar in all sites (~4-7 μg L(-1)), both control and hunting, and higher than reference values for aquatic media. Lead was also present in vegetation, including grasses and rice crops, in almost all cases. Most soil-collection sites were slightly acidic, and were frequently flooded. These results strongly suggest that metallic Pb from spent shot is oxidized and dissolved due to wetland conditions. Thus, the pollutant is readily mobilized and distributed across all wetland areas, effectively homogenizing its concentration in locations with and without hunting activities. The replacement of lead by nontoxic materials in pellets appears to be the only effective way to prevent Pb pollution in wetlands. Copyright © 2015 Elsevier B.V. All rights reserved.

Use of bean husk as an easily digestible fiber source for activating the fibrolytic rumen bacterium Fibrobacter succinogenes and rice straw digestion.

PubMed

Fuma, Ryosuke; Oyaizu, Shinya; Nukui, Yoko; Ngwe, Tin; Shinkai, Takumi; Koike, Satoshi; Kobayashi, Yasuo

2012-10-01

A series of in sacco and in vitro studies were carried out to evaluate bean husks for activation of fibrolytic rumen bacteria and rice straw digestion. First, lablab bean husk, chickpea husk and rice straw were suspended in the rumen of sheep to analyze the bacterial consortium developed on each fiber source. Known members of fiber-associating bacteria were found on both lablab bean husk and rice straw, but some of these bacteria were lacking on chickpea husk. Second, a pure culture study was carried out using six strains of Fibrobacter succinogenes. Both husks stimulated the growth of all tested strains, including a strain that did not grow on rice straw. The strain OS128 that showed the highest growth on rice straw displayed even higher growth on lablab bean husk without a time lag. Finally, two-step incubations were carried out to determine whether prior incubation of rumen fluid with husks stimulates subsequent rice straw digestion. Higher digestibility of rice straw was recorded in the second-round incubation following the first incubation with bean husks. These results suggest that the tested bean husks improve the digestion of rice straw by activating fibrolytic F. succinogenes and other associated bacteria. © 2012 The Authors. Animal Science Journal © 2012 Japanese Society of Animal Science.

Temporal variations in arsenic uptake by rice plants in Bangladesh: the role of iron plaque in paddy fields irrigated with groundwater