The Effectiveness of Asian Bush Mosquito (Aedes japonicus japonicus) Control Actions in Colonised Peri-urban Areas in the Netherlands

PubMed Central

Ibañez-Justicia, A; Teekema, S; den Hartog, W; Jacobs, F; Dik, M; Stroo, A

2018-01-01

Abstract The Asian bush mosquito (Aedes japonicus japonicus (Theobald)) is an invasive mosquito species in Europe. In 2012, it was for the first time detected in the Netherlands, in the municipality of Lelystad. After further research, thousands of specimens were found in the surrounding peri-urban areas of the city. A targeted mosquito control campaign began in 2015 with the objective of reducing populations in locations with the highest concentrations of Ae. japonicus breeding sites: allotment garden complexes. Mosquito control consisted of source reduction combined with application of the larvicide Vectomax in breeding sites. At eight complexes, mosquito control effectiveness has been systematically measured by sampling larvae from breeding sites. Six measurements were performed between 2015 and 2016. Results show that the effectiveness of mosquito control actions was similar in all treated allotment gardens and resulted in a significant reduction in Ae. japonicus larval abundance. Rain barrels at the allotments represent the most frequent breeding site in Lelystad, but every water filled artificial container is a potential breeding site for the species. Ae. japonicus was not found in the samples taken in other allotment gardens in the province of Flevoland; however, the collection methodology used proven to be effective in detecting this species when it has newly colonized surrounding areas. Targeted mosquito control actions at the breeding sites are crucial for successful reduction of populations of an invasive mosquito species, and systematic measurements of the effectiveness, is in this case, the base to understand the dynamics of Ae. japonicus populations after mosquito control. PMID:29452383

Polysaccharide degradation systems of the saprophytic bacterium Cellvibrio japonicus

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gardner, Jeffrey G.

Study of recalcitrant polysaccharide degradation by bacterial systems is critical for understanding biological processes such as global carbon cycling, nutritional contributions of the human gut microbiome, and the production of renewable fuels and chemicals. One bacterium that has a robust ability to degrade polysaccharides is the Gram-negative saprophyte Cellvibrio japonicus. A bacterium with a circuitous history, C. japonicus underwent several taxonomy changes from an initially described Pseudomonas sp. Most of the enzymes described in the pre-genomics era have also been renamed. Furthermore, this review aims to consolidate the biochemical, structural, and genetic data published on C. japonicus and its remarkablemore » ability to degrade cellulose, xylan, and pectin substrates. Initially, C. japonicus carbohydrate-active enzymes were studied biochemically and structurally for their novel polysaccharide binding and degradation characteristics, while more recent systems biology approaches have begun to unravel the complex regulation required for lignocellulose degradation in an environmental context. Also included is a discussion for the future of C. japonicus as a model system, with emphasis on current areas unexplored in terms of polysaccharide degradation and emerging directions for C. japonicus in both environmental and biotechnological applications.« less

Polysaccharide degradation systems of the saprophytic bacterium Cellvibrio japonicus

DOE PAGES

Gardner, Jeffrey G.

2016-06-04

Study of recalcitrant polysaccharide degradation by bacterial systems is critical for understanding biological processes such as global carbon cycling, nutritional contributions of the human gut microbiome, and the production of renewable fuels and chemicals. One bacterium that has a robust ability to degrade polysaccharides is the Gram-negative saprophyte Cellvibrio japonicus. A bacterium with a circuitous history, C. japonicus underwent several taxonomy changes from an initially described Pseudomonas sp. Most of the enzymes described in the pre-genomics era have also been renamed. Furthermore, this review aims to consolidate the biochemical, structural, and genetic data published on C. japonicus and its remarkablemore » ability to degrade cellulose, xylan, and pectin substrates. Initially, C. japonicus carbohydrate-active enzymes were studied biochemically and structurally for their novel polysaccharide binding and degradation characteristics, while more recent systems biology approaches have begun to unravel the complex regulation required for lignocellulose degradation in an environmental context. Also included is a discussion for the future of C. japonicus as a model system, with emphasis on current areas unexplored in terms of polysaccharide degradation and emerging directions for C. japonicus in both environmental and biotechnological applications.« less

Novel SINEs families in Medicago truncatula and Lotus japonicus: bioinformatic analysis.

PubMed

Gadzalski, Marek; Sakowicz, Tomasz

2011-07-01

Although short interspersed elements (SINEs) were discovered nearly 30 years ago, the studies of these genomic repeats were mostly limited to animal genomes. Very little is known about SINEs in legumes--one of the most important plant families. Here we report identification, genomic distribution and molecular features of six novel SINE elements in Lotus japonicus (named LJ_SINE-1, -2, -3) and Medicago truncatula (MT_SINE-1, -2, -3), model species of legume. They possess all the structural features commonly found in short interspersed elements including RNA polymerase III promoter, polyA tail and flanking repeats. SINEs described here are present in low to moderate copy numbers from 150 to 3000. Bioinformatic analyses were used to searched public databases, we have shown that three of new SINE elements from M. truncatula seem to be characteristic of Medicago and Trifolium genera. Two SINE families have been found in L. japonicus and one is present in both M. truncatula and L. japonicus. In addition, we are discussing potential activities of the described elements. Copyright © 2011 Elsevier B.V. All rights reserved.

Antiviral effects of Stichopus japonicus acid mucopolysaccharide on hepatitis B virus transgenic mice

NASA Astrophysics Data System (ADS)

Xin, Yongning; Li, Wei; Lu, Linlin; Zhou, Li; Victor, David W.; Xuan, Shiying

2016-08-01

Hepatitis B virus (HBV) is a significant global pathogen and efficient cure for HBV patients is still a challenging goal. We previously reported that acidic mucopolysaccharide from stichopus japonicus selenka (SJAMP) could inhibit HBsAg and HBeAg expression in vitro. However, the potential anti-HBV effects of SJAMP in vivo have not yet been explored. In this study, we show that SJAMP exhibits potent anti-HBV activity in HBV transgenic mice in a dose-dependent manner. Specifically, sixty HBV transgenic male BALB/c mice were randomly selected to receive the treatment of PBS, low dose SJAMP (30 mg kg-1), middle dose SJAMP (40 mg kg-1), high dose SJAMP (50 mg kg-1) and IFN (45 IU kg-1) for 30 d. SJAMP treatment suppressed serum HBV-DNA, and liver HBsAg and HBcAg levels in HBV-transgenic mice. The present study highlights the potential application of SJAMP in HBV therapy.

Comparative transcriptome analysis of three color variants of the sea cucumber Apostichopus japonicus.

PubMed

Jo, Jihoon; Park, Jongsun; Lee, Hyun-Gwan; Kern, Elizabeth M A; Cheon, Seongmin; Jin, Soyeong; Park, Joong-Ki; Cho, Sung-Jin; Park, Chungoo

2016-08-01

The sea cucumber Apostichopus japonicus Selenka 1867 represents an important resource in biomedical research, traditional medicine, and the seafood industry. Much of the commercial value of A. japonicus is determined by dorsal/ventral color variation (red, green, and black), yet the taxonomic relationships between these color variants are not clearly understood. We performed the first comparative analysis of de novo assembled transcriptome data from three color variants of A. japonicus. Using the Illumina platform, we sequenced nearly 177,596,774 clean reads representing a total of 18.2Gbp of sea cucumber transcriptome. A comparison of over 0.3 million transcript scaffolds against the Uniprot/Swiss-Prot database yielded 8513, 8602, and 8588 positive matches for green, red, and black body color transcriptomes, respectively. Using the Panther gene classification system, we assessed an extensive and diverse set of expressed genes in three color variants and found that (1) among the three color variants of A. japonicus, genes associated with RNA binding protein, oxidoreductase, nucleic acid binding, transferase, and KRAB box transcription factor were most commonly expressed; and (2) the main protein functional classes are differently regulated in all three color variants (extracellular matrix protein and phosphatase for green color, transporter and potassium channel for red color, and G-protein modulator and enzyme modulator for black color). This work will assist in the discovery and annotation of novel genes that play significant morphological and physiological roles in color variants of A. japonicus, and these sequence data will provide a useful set of resources for the rapidly growing sea cucumber aquaculture industry. Copyright © 2016 Elsevier B.V. All rights reserved.

Taxonomic review of Argentine mackerel Scomber japonicus (Houttuyn, 1782) by phylogenetic analysis

PubMed Central

Trucco, María Inés; Buratti, Claudio César

2017-01-01

Taxonomically, Argentine mackerels were first considered as Scomber japonicus marplatensis and later as Scomber japonicus Houttuyn 1782, although, in the last years, different studies have suggested that South Atlantic mackerel species belongs to Scomber colias Gmelin 1789. These latter results, incorporated in the main fish databases (FishBase and Catalog of Fishes), promoted a phylogenetic study using cytochrome c oxidase I (COI) gene sequences taken from the Barcode of Life (FISH-BOL) database. Thus, 76 sequences of S. japonicus, S. colias, S. australasicus and S. scombrus from different regions were used; including 3 from Sarda sarda as outgroup. Among S. japonicus selected sequences are those corresponding to the Argentine mackerels collected in 2007. Phylogenetic trees were obtained by neighbor joining and maximum likelihood methods and a network of haplotypes was reconstructed to analyze the relationship between species. The results showed the clear differentiation of S. australasicus, S. scombrus and S. japonicus from the Pacific while S. japonicus from Argentina was included in the S. colias group, with genetic differences corresponding to conspecific populations (0.1%). Four of the five Argentine specimens shared the same haplotype with S. colias, and none were shared with S. japonicus from the Pacific. These results suggest that the current specific name of Argentine mackerel S. japonicus should be changed to S. colias, in agreement with several genetic studies carried out with species of the genus Scomber. PMID:29071283

Experimental transmission of St. Louis encephalitis virus by Ochlerotatus j. japonicus.

PubMed

Sardelis, Michael R; Turell, Michael J; Andre, Richard G

2003-06-01

Ochlerotatus japonicus japonicus a newly discovered nonindigenous mosquito species in North America, and a colonized strain of Culex pipiens were compared for their vector competence for St. Louis encephalitis virus (SLE). Infection rates in Oc. j. japonicus were 0-33% after feeding on chickens with viremias between 10(4.1) and 10(4.7) plaque-forming units (PFU)/ml of blood. In comparison, infection rates were 12-94% for Cx. pipiens that fed on the same chickens. When fed on chickens with viremias between 10(5.3) and 10(5.6) PFU/ml of blood, infection rates for Oc. j. japonicus and Cx. pipiens were similar, 96% and 100%, respectively. After 12-14 days of extrinsic incubation at 26 degrees C, all 34 infected Oc. j. japonicus had a disseminated infection. In contrast, only 23 (43%) of 54 infected Cx. pipiens had a disseminated infection after feeding on the same chickens. If they developed a disseminated infection, both species efficiently transmitted (> or = 87%) SLE. Estimated transmission rates at viral doses sufficient to infect both of the tested species were 29-84% for Oc. j. japonicus and 30-50% for Cx. pipiens. Because of its continued geographic expansion, field and laboratory evidence incriminating it as a vector of the closely related West Nile virus, and its ability to transmit SLE in the laboratory, Oc. j. japonicus should be considered as a potential enzootic or epizootic vector of SLE.

Design of prospective study of acute coronary syndrome hospitalization after smoking ban in public places in Hyogo prefecture: comparison with Gifu, a prefecture without a public smoking ban.

PubMed

Sato, Yukihito; Minatoguchi, Shinya; Nishigaki, Kazuhiko; Hirata, Ken-ichi; Masuyama, Tohru; Furukawa, Yutaka; Uematsu, Masaaki; Yoshikawa, Junichi; Otsuji, Satoru; Iida, Mami; Fujiwara, Hisayoshi

2014-02-01

Hyogo is the second prefecture, after Kanagawa, to enact a smoking ban in public places in Japan. The effect of this smoking ban on acute coronary syndrome (ACS) has not been evaluated. Changes in the annual number of ACS hospital cases in Hyogo Prefecture, before and after the enactment of the prefectural legislative ban on smoking in public places, are to be compared with those in Gifu Prefecture, where there is no smoking-ban legislation. Consecutive Hyogo residents with ACS, admitted to 33 major hospitals in the Hanshin-Awaji-Kobe district, which covers 56% of the population, during the 12 months before implementation of the legislation (April 2012 through March 2013) and during the same 24 months thereafter (April 2013 through March 2015) will be enrolled. Consecutive patients with ACS, who are Gifu residents, treated at the 20 major hospitals in Gifu Prefecture will be enrolled as geographical controls. The primary endpoint is the change in number of ACS admissions from April 2012 through March 2015, considering the periods before and after the smoking-ban legislation in Hyogo prefecture. Our study has certain strengths: (1) This is the first large Japanese study of ACS registry with smoking-ban legislation. (2) Major hospitals in the Hanshin-Awaji-Kobe district are included. (3) The data will cover 3 years including 1 year before legislation enactment. (4) The data will be compared with those of Gifu Prefecture, where smoking-ban legislation will not be enacted. (5) The very large database makes possible analysis of subgroups based on age and gender. Copyright © 2013 Japanese College of Cardiology. Published by Elsevier Ltd. All rights reserved.

Newly discovered population of Aedes japonicus japonicus (Diptera: Culicidae) in Upper Bavaria, Germany, and Salzburg, Austria, is closely related to the Austrian/Slovenian bush mosquito population.

PubMed

Zielke, Dorothee E; Walther, Doreen; Kampen, Helge

2016-03-21

The German mosquito surveillance instrument 'Mueckenatlas' requests the general public to collect and submit mosquito specimens. Among these, increasing numbers of individuals of invasive species have been registered. Specimens of the Asian bush mosquito Aedes japonicus japonicus submitted from German Upper Bavaria, where this species had not previously been recorded, triggered regional monitoring in mid-2015. The search for Ae. j. japonicus breeding sites and developmental stages concentrated on cemeteries in the municipality of origin of the submitted specimens and, subsequently, in the whole region. A virtual grid consisting of 10 × 10 km(2) cells in which up to three cemeteries were checked, was laid over the region. A cell was considered positive as soon as Ae. j. japonicus larvae were detected, and regarded negative when no larvae could be found in any of the cemeteries inspected. All cells surrounding a positive cell were screened accordingly. A subset of collected Aedes j. japonicus specimens was subjected to microsatellite and nad4 sequence analyses, and obtained data were compared to individuals from previously discovered European populations. Based on the grid cells, an area of approximately 900 km(2) was populated by Ae. j. japonicus in Upper Bavaria and neighbouring Austria. Genetic analyses of microsatellites and nad4 gene sequences generated one genotype out of two previously described for Europe and three haplotypes, one of which had previously been found in Europe only in Ae. j. japonicus samples from a population in East Austria and Slovenia. The genetic analysis suggests the new population is closely related to the Austrian/Slovenian population. As Ae. j. japonicus is well adapted to temperate climates, it has a strong tendency to expand and to colonise new territories in Central Europe, which is facilitated by human-mediated, passive transportation. The new population in Upper Bavaria/Austria is the seventh separate population described in

Evaluation of body weight of sea cucumber Apostichopus japonicus by computer vision

NASA Astrophysics Data System (ADS)

Liu, Hui; Xu, Qiang; Liu, Shilin; Zhang, Libin; Yang, Hongsheng

2015-01-01

A postichopus japonicus (Holothuroidea, Echinodermata) is an ecological and economic species in East Asia. Conventional biometric monitoring method includes diving for samples and weighing above water, with highly variable in weight measurement due to variation in the quantity of water in the respiratory tree and intestinal content of this species. Recently, video survey method has been applied widely in biometric detection on underwater benthos. However, because of the high flexibility of A. japonicus body, video survey method of monitoring is less used in sea cucumber. In this study, we designed a model to evaluate the wet weight of A. japonicus, using machine vision technology combined with a support vector machine (SVM) that can be used in field surveys on the A. japonicus population. Continuous dorsal images of free-moving A. japonicus individuals in seawater were captured, which also allows for the development of images of the core body edge as well as thorn segmentation. Parameters that include body length, body breadth, perimeter and area, were extracted from the core body edge images and used in SVM regression, to predict the weight of A. japonicus and for comparison with a power model. Results indicate that the use of SVM for predicting the weight of 33 A. japonicus individuals is accurate ( R 2=0.99) and compatible with the power model ( R 2 =0.96). The image-based analysis and size-weight regression models in this study may be useful in body weight evaluation of A. japonicus in lab and field study.

Genome Structure of the Legume, Lotus japonicus

PubMed Central

Sato, Shusei; Nakamura, Yasukazu; Kaneko, Takakazu; Asamizu, Erika; Kato, Tomohiko; Nakao, Mitsuteru; Sasamoto, Shigemi; Watanabe, Akiko; Ono, Akiko; Kawashima, Kumiko; Fujishiro, Tsunakazu; Katoh, Midori; Kohara, Mitsuyo; Kishida, Yoshie; Minami, Chiharu; Nakayama, Shinobu; Nakazaki, Naomi; Shimizu, Yoshimi; Shinpo, Sayaka; Takahashi, Chika; Wada, Tsuyuko; Yamada, Manabu; Ohmido, Nobuko; Hayashi, Makoto; Fukui, Kiichi; Baba, Tomoya; Nakamichi, Tomoko; Mori, Hirotada; Tabata, Satoshi

2008-01-01

The legume Lotus japonicus has been widely used as a model system to investigate the genetic background of legume-specific phenomena such as symbiotic nitrogen fixation. Here, we report structural features of the L. japonicus genome. The 315.1-Mb sequences determined in this and previous studies correspond to 67% of the genome (472 Mb), and are likely to cover 91.3% of the gene space. Linkage mapping anchored 130-Mb sequences onto the six linkage groups. A total of 10 951 complete and 19 848 partial structures of protein-encoding genes were assigned to the genome. Comparative analysis of these genes revealed the expansion of several functional domains and gene families that are characteristic of L. japonicus. Synteny analysis detected traces of whole-genome duplication and the presence of synteny blocks with other plant genomes to various degrees. This study provides the first opportunity to look into the complex and unique genetic system of legumes. PMID:18511435

Effects of potential probiotic Bacillus cereus EN25 on growth, immunity and disease resistance of juvenile sea cucumber Apostichopus japonicus.

PubMed

Zhao, Yancui; Yuan, Lei; Wan, Junli; Sun, Zhenxing; Wang, Yiyan; Sun, Hushan

2016-02-01

This study was conducted to determine effects of potential probiotic Bacillus cereus EN25 (isolated from mud of sea cucumber culturing water bodies) on growth, immunity and disease resistance against Vibrio splendidus infection in juvenile sea cucumbers Apostichopus japonicus. Animals were respectively fed diets with B. cereus EN25 at 0 (control), 10(5), 10(7) and 10(9) CFU/g for 30 days. Results showed that dietary B. cereus EN25 had no significant effects on growth, total coelomocytes counts and acid phosphatase activity of A. japonicus (P > 0.05). Dietary EN25 at 10(7) CFU/g had significantly improved the phagocytosis, respiratory burst activity and total nitric oxide synthase activity of animals (P < 0.05). Compared to control, dietary EN25 at 10(5) or 10(7) CFU/g had no significant effects on superoxide dismutase activity of A. japonicus (P > 0.05), whereas dietary EN25 at 10(9) CFU/g had significantly decreased its activity (P < 0.05). The cumulative mortality after V. splendidus challenge decreased significantly in sea cucumbers fed with EN25 at 10(7) CFU/g (P < 0.05). The present study confirmed dietary B. cereus EN25 at 10(7) CFU/g could significantly improve immunity and disease resistance in juvenile A. japonicus. Copyright © 2015 Elsevier Ltd. All rights reserved.

The WRKY Transcription Factor Genes in Lotus japonicus.

PubMed

Song, Hui; Wang, Pengfei; Nan, Zhibiao; Wang, Xingjun

2014-01-01

WRKY transcription factor genes play critical roles in plant growth and development, as well as stress responses. WRKY genes have been examined in various higher plants, but they have not been characterized in Lotus japonicus. The recent release of the L. japonicus whole genome sequence provides an opportunity for a genome wide analysis of WRKY genes in this species. In this study, we identified 61 WRKY genes in the L. japonicus genome. Based on the WRKY protein structure, L. japonicus WRKY (LjWRKY) genes can be classified into three groups (I-III). Investigations of gene copy number and gene clusters indicate that only one gene duplication event occurred on chromosome 4 and no clustered genes were detected on chromosomes 3 or 6. Researchers previously believed that group II and III WRKY domains were derived from the C-terminal WRKY domain of group I. Our results suggest that some WRKY genes in group II originated from the N-terminal domain of group I WRKY genes. Additional evidence to support this hypothesis was obtained by Medicago truncatula WRKY (MtWRKY) protein motif analysis. We found that LjWRKY and MtWRKY group III genes are under purifying selection, suggesting that WRKY genes will become increasingly structured and functionally conserved.

The WRKY Transcription Factor Genes in Lotus japonicus

PubMed Central

Wang, Pengfei; Wang, Xingjun

2014-01-01

WRKY transcription factor genes play critical roles in plant growth and development, as well as stress responses. WRKY genes have been examined in various higher plants, but they have not been characterized in Lotus japonicus. The recent release of the L. japonicus whole genome sequence provides an opportunity for a genome wide analysis of WRKY genes in this species. In this study, we identified 61 WRKY genes in the L. japonicus genome. Based on the WRKY protein structure, L. japonicus WRKY (LjWRKY) genes can be classified into three groups (I–III). Investigations of gene copy number and gene clusters indicate that only one gene duplication event occurred on chromosome 4 and no clustered genes were detected on chromosomes 3 or 6. Researchers previously believed that group II and III WRKY domains were derived from the C-terminal WRKY domain of group I. Our results suggest that some WRKY genes in group II originated from the N-terminal domain of group I WRKY genes. Additional evidence to support this hypothesis was obtained by Medicago truncatula WRKY (MtWRKY) protein motif analysis. We found that LjWRKY and MtWRKY group III genes are under purifying selection, suggesting that WRKY genes will become increasingly structured and functionally conserved. PMID:24745006

Contributions of temporal segregation, oviposition choice, and non-additive effects of competitors to invasion success of Aedes japonicus (Diptera: Culicidae) in North America

PubMed Central

Murrell, Ebony G.; Noden, Bruce H.; Juliano, Steven A.

2015-01-01

The mosquito Aedes japonicus (Diptera: Culicidae) has spread rapidly through North America since its introduction in the 1990s. The mechanisms underlying its establishment in container communities occupied by competitors Aedes triseriatus and Aedes albopictus are unclear. Possibilities include (A) temporal separation of A. japonicus from other Aedes, (B) oviposition avoidance by A. japonicus of sites containing heterospecific Aedes larvae, and (C) non-additive competitive effects in assemblages of multiple Aedes. Containers sampled throughout the summer in an oak-hickory forest near Eureka, MO showed peak abundance for A. japonicus occurring significantly earlier in the season than either of the other Aedes species. Despite this, A. japonicus co-occurred with one other Aedes species in 53 % of samples when present, and co-occurred with both other Aedes in 18 % of samples. In a field oviposition experiment, A. japonicus laid significantly more eggs in forest edge containers than in forest interior containers, but did not avoid containers with low or high densities of larvae of A. triseriatus, A. albopictus, or both, compared to containers without larvae. Interspecific competitive effects (measured as decrease in the index of performance, λ′) of A. triseriatus or A. albopictus alone on A. japonicus larvae were not evident at the densities used, but the effect of both Aedes combined was significantly negative and super-additive of effects of individual interspecific competitors. Thus, neither oviposition avoidance of competitors nor non-additive competitive effects contribute to the invasion success of A. japonicus in North America. Distinct seasonal phenology may reduce competitive interactions with resident Aedes. PMID:26101466

A new focus of Aedes japonicus japonicus (Theobald, 1901) (Diptera, Culicidae) distribution in Western Germany: rapid spread or a further introduction event?

PubMed Central

2012-01-01

Background The Asian bush mosquito, Aedes japonicus japonicus, a potential vector of several viruses, was first detected in Germany in 2008 on the Swiss-German border. In the following years, this invasive species apparently succeeded in establishing populations in southern Germany and in spreading northwards. In 2011, its distribution area already covered large areas of the federal state of Baden-Wurttemberg, and its northernmost German collection point was reported to be close to Stuttgart. Several independent submissions to our laboratories of Ae. j. japonicus specimens in July 2012, originating from the same area in the federal state of North Rhine-Westphalia, western Germany, prompted us to carry out an immediate surveillance in this region in the expectation of finding a further distribution focus of Ae. j. japonicus in Germany. Methods After inspecting the places of residence of the collectors of the submitted mosquito specimens, all kinds of water containers in 123 cemeteries in surrounding towns and villages were checked for mosquito developmental stages. These were collected and kept to produce adults for morphological species identification. One specimen per collection site was identified genetically by COI sequence analysis. Results Aedes j. japonicus adults and immature stages were found in 36 towns/villages that were checked (29%) over an area of approximately 2,000 km2 in southern North Rhine-Westphalia and northern Rhineland Palatinate. The species could not be demonstrated further south when monitoring towards the northernmost previous collection sites in southern Germany. It therefore remains to be elucidated whether the species has entered western Germany from the south, from Belgium in the west where it has been demonstrated to occur locally since 2002, or through a new introduction. Conclusions Aedes j. japonicus is obviously much more widely distributed in Germany than previously thought. It appears to be well adapted, to have a strong

Systems analysis in Cellvibrio japonicus resolves predicted redundancy of β-glucosidases and determines essential physiological functions: Functional analysis of C. japonicus β-glucosidases

DOE PAGES

Nelson, Cassandra E.; Rogowski, Artur; Morland, Carl; ...

2017-02-28

Degradation of polysaccharides forms an essential arc in the carbon cycle, provides a percentage of our daily caloric intake, and is a major driver in the renewable chemical industry. Microorganisms proficient at degrading insoluble polysaccharides possess large numbers of carbohydrate active enzymes, many of which have been categorized as functionally redundant. Here we present data that suggests that carbohydrate active enzymes that have overlapping enzymatic activities can have unique, non-overlapping biological functions in the cell. Our comprehensive study to understand cellodextrin utilization in the soil saprophyte Cellvibrio japonicus found that only one of four predicted β-glucosidases is required in amore » physiological context. Gene deletion analysis indicated that only the cel3B gene product is essential for efficient cellodextrin utilization in C. japonicus and is constitutively expressed at high levels. Interestingly, expression of individual β-glucosidases in Escherichia coli K-12 enabled this non-cellulolytic bacterium to be fully capable of using cellobiose as a sole carbon source. Furthermore, enzyme kinetic studies indicated that the Cel3A enzyme is significantly more active than the Cel3B enzyme on the oligosaccharides but not disaccharides. Finally, our approach for parsing related carbohydrate active enzymes to determine actual physiological roles in the cell can be applied to other polysaccharide-degradation systems.« less

29 CFR 102.129 - Communications prohibited.

Code of Federal Regulations, 2010 CFR

2010-07-01

... § 102.114(a). (b) Such communications, when oral, unless advance notice thereof is given by the... 29 Labor 2 2010-07-01 2010-07-01 false Communications prohibited. 102.129 Section 102.129 Labor... Communications § 102.129 Communications prohibited. Except as provided in § 102.130, ex parte communications...

46 CFR 129.350 - Batteries-general.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 4 2010-10-01 2010-10-01 false Batteries-general. 129.350 Section 129.350 Shipping... INSTALLATIONS Power Sources and Distribution Systems § 129.350 Batteries—general. (a) Wherever a battery is charged, there must be natural or induced ventilation to dissipate the gases generated. (b) Each battery...

46 CFR 129.350 - Batteries-general.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 4 2014-10-01 2014-10-01 false Batteries-general. 129.350 Section 129.350 Shipping... INSTALLATIONS Power Sources and Distribution Systems § 129.350 Batteries—general. (a) Wherever a battery is charged, there must be natural or induced ventilation to dissipate the gases generated. (b) Each battery...

46 CFR 129.350 - Batteries-general.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 4 2013-10-01 2013-10-01 false Batteries-general. 129.350 Section 129.350 Shipping... INSTALLATIONS Power Sources and Distribution Systems § 129.350 Batteries—general. (a) Wherever a battery is charged, there must be natural or induced ventilation to dissipate the gases generated. (b) Each battery...

46 CFR 129.350 - Batteries-general.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 4 2012-10-01 2012-10-01 false Batteries-general. 129.350 Section 129.350 Shipping... INSTALLATIONS Power Sources and Distribution Systems § 129.350 Batteries—general. (a) Wherever a battery is charged, there must be natural or induced ventilation to dissipate the gases generated. (b) Each battery...

46 CFR 129.350 - Batteries-general.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 4 2011-10-01 2011-10-01 false Batteries-general. 129.350 Section 129.350 Shipping... INSTALLATIONS Power Sources and Distribution Systems § 129.350 Batteries—general. (a) Wherever a battery is charged, there must be natural or induced ventilation to dissipate the gases generated. (b) Each battery...

The complete chloroplast genome sequence of Euonymus japonicus (Celastraceae).

PubMed

Choi, Kyoung Su; Park, SeonJoo

2016-09-01

The complete chloroplast (cp) genome sequence of the Euonymus japonicus, the first sequenced of the genus Euonymus, was reported in this study. The total length was 157 637 bp, containing a pair of 26 678 bp inverted repeat region (IR), which were separated by small single copy (SSC) region and large single copy (LSC) region of 18 340 bp and 85 941 bp, respectively. This genome contains 107 unique genes, including 74 coding genes, four rRNA genes, and 29 tRNA genes. Seventeen genes contain intron of E. japonicus, of which three genes (clpP, ycf3, and rps12) include two introns. The maximum likelihood (ML) phylogenetic analysis revealed that E. japonicus was closely related to Manihot and Populus.

Establishment of Ochlerotatus japonicus (Diptera: Culicidae) in Ontario, Canada.

PubMed

Thielman, Aynsley; Hunter, Fiona F

2006-03-01

Ochlerotatus (Finlaya) japonicus (Theobold) is newly established in Ontario, Canada. It was first discovered in 2001 during the province-wide West Nile virus mosquito surveillance program implemented by the Ontario Ministry of Health and Long Term Care. Although the numbers of adults trapped in CDC light traps were low in 2001 and 2002, they increased during the 2003 and 2004 surveillance seasons. Oc. japonicus larvae also have been collected in large numbers in the Niagara Peninsula in a variety of natural and artificial containers. The number of health units with records for Oc. japonicus has increased over the 4 yr of surveillance, illustrating the ability of this species to rapidly extend its range. As a potential arboviral bridge vector, its establishment in Ontario requires further study and should be considered a public health concern.

[Principal component analysis and cluster analysis of inorganic elements in sea cucumber Apostichopus japonicus].

PubMed

Liu, Xiao-Fang; Xue, Chang-Hu; Wang, Yu-Ming; Li, Zhao-Jie; Xue, Yong; Xu, Jie

2011-11-01

The present study is to investigate the feasibility of multi-elements analysis in determination of the geographical origin of sea cucumber Apostichopus japonicus, and to make choice of the effective tracers in sea cucumber Apostichopus japonicus geographical origin assessment. The content of the elements such as Al, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, As, Se, Mo, Cd, Hg and Pb in sea cucumber Apostichopus japonicus samples from seven places of geographical origin were determined by means of ICP-MS. The results were used for the development of elements database. Cluster analysis(CA) and principal component analysis (PCA) were applied to differentiate the sea cucumber Apostichopus japonicus geographical origin. Three principal components which accounted for over 89% of the total variance were extracted from the standardized data. The results of Q-type cluster analysis showed that the 26 samples could be clustered reasonably into five groups, the classification results were significantly associated with the marine distribution of the sea cucumber Apostichopus japonicus samples. The CA and PCA were the effective methods for elements analysis of sea cucumber Apostichopus japonicus samples. The content of the mineral elements in sea cucumber Apostichopus japonicus samples was good chemical descriptors for differentiating their geographical origins.

Seasonal parasitism and host specificity of Trissolcus japonicus in northern China.

PubMed

Zhang, Jinping; Zhang, Feng; Gariepy, Tara; Mason, Peter; Gillespie, Dave; Talamas, Elijah; Haye, Tim

2017-01-01

The brown marmorated stink bug, Halyomorpha halys (Stål), native to China, Japan, and Korea, has emerged as a harmful invasive pest of a variety of crops in North America and Europe. The Asian egg parasitoid Trissolcus japonicus has been identified as the most promising agent for classical biological control of invasive H. halys populations. A 4-year study evaluated the fundamental and ecological host ranges of T. japonicus as well as its phenology and impact on H. halys populations in fruit orchards in its native range in northern China. In laboratory no-choice tests, developmental suitability of eight non-target host species for T. japonicus was demonstrated by the successful production of progeny on the majority (>85%) of non-target host species tested. In field-collected, naturally laid egg masses, T. japonicus was the most abundant parasitoid associated with H. halys and Dolycoris baccarum , but was also sporadically found in Plautia crossota . Furthermore, it was regularly reared from sentinel egg masses of Menida violacea , Arma chinensis , and Carbula eoa . The only species that did not support development in the laboratory and field was Cappaea tibialis . Besides the benefit of having a high impact on H. halys populations in Northern China, the risk assessment conducted in the area of origin indicates that native Pentatomidae in North America and Europe could be negatively impacted by T. japonicus . Whether the benefits of T. japonicus outweigh the possible risks will have to be evaluated based on the outcome of additional host range studies in the two invaded regions.

14 CFR 129.29 - Smoking prohibitions.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 14 Aeronautics and Space 3 2013-01-01 2013-01-01 false Smoking prohibitions. 129.29 Section 129.29... AND FOREIGN OPERATORS OF U.S.-REGISTERED AIRCRAFT ENGAGED IN COMMON CARRIAGE General § 129.29 Smoking prohibitions. (a) No person may smoke and no operator may permit smoking in any aircraft lavatory. (b) Unless...

14 CFR 129.29 - Smoking prohibitions.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Smoking prohibitions. 129.29 Section 129.29... AND FOREIGN OPERATORS OF U.S.-REGISTERED AIRCRAFT ENGAGED IN COMMON CARRIAGE General § 129.29 Smoking prohibitions. (a) No person may smoke and no operator may permit smoking in any aircraft lavatory. (b) Unless...

14 CFR 129.29 - Smoking prohibitions.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 14 Aeronautics and Space 3 2014-01-01 2014-01-01 false Smoking prohibitions. 129.29 Section 129.29... AND FOREIGN OPERATORS OF U.S.-REGISTERED AIRCRAFT ENGAGED IN COMMON CARRIAGE General § 129.29 Smoking prohibitions. (a) No person may smoke and no operator may permit smoking in any aircraft lavatory. (b) Unless...

14 CFR 129.29 - Smoking prohibitions.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Smoking prohibitions. 129.29 Section 129.29... AND FOREIGN OPERATORS OF U.S.-REGISTERED AIRCRAFT ENGAGED IN COMMON CARRIAGE General § 129.29 Smoking prohibitions. (a) No person may smoke and no operator may permit smoking in any aircraft lavatory. (b) Unless...

14 CFR 129.29 - Smoking prohibitions.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 14 Aeronautics and Space 3 2012-01-01 2012-01-01 false Smoking prohibitions. 129.29 Section 129.29... AND FOREIGN OPERATORS OF U.S.-REGISTERED AIRCRAFT ENGAGED IN COMMON CARRIAGE General § 129.29 Smoking prohibitions. (a) No person may smoke and no operator may permit smoking in any aircraft lavatory. (b) Unless...

Seasonal parasitism and host specificity of Trissolcus japonicus in northern China

USDA-ARS?s Scientific Manuscript database

The Asian egg parasitoid Trissolcus japonicus is considered the most promising species for classical biological control of Halyomorpha halys. We investigated the fundamental and ecological host range of T. japonicus in northern China to define its host specificity, and we determined that T. japonicu...

27 CFR 21.129 - Spearmint oil, terpeneless.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Spearmint oil, terpeneless. 21.129 Section 21.129 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU....129 Spearmint oil, terpeneless. (a) Carvone content. Not less than 85 percent by weight. (b...

27 CFR 21.129 - Spearmint oil, terpeneless.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 27 Alcohol, Tobacco Products and Firearms 1 2011-04-01 2011-04-01 false Spearmint oil, terpeneless. 21.129 Section 21.129 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU....129 Spearmint oil, terpeneless. (a) Carvone content. Not less than 85 percent by weight. (b...

27 CFR 21.129 - Spearmint oil, terpeneless.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 27 Alcohol, Tobacco Products and Firearms 1 2014-04-01 2014-04-01 false Spearmint oil, terpeneless. 21.129 Section 21.129 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU....129 Spearmint oil, terpeneless. (a) Carvone content. Not less than 85 percent by weight. (b...

27 CFR 21.129 - Spearmint oil, terpeneless.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 27 Alcohol, Tobacco Products and Firearms 1 2013-04-01 2013-04-01 false Spearmint oil, terpeneless. 21.129 Section 21.129 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU....129 Spearmint oil, terpeneless. (a) Carvone content. Not less than 85 percent by weight. (b...

27 CFR 21.129 - Spearmint oil, terpeneless.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 27 Alcohol, Tobacco Products and Firearms 1 2012-04-01 2012-04-01 false Spearmint oil, terpeneless. 21.129 Section 21.129 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU....129 Spearmint oil, terpeneless. (a) Carvone content. Not less than 85 percent by weight. (b...

46 CFR 129.360 - Semiconductor-rectifier systems.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 4 2011-10-01 2011-10-01 false Semiconductor-rectifier systems. 129.360 Section 129.360... INSTALLATIONS Power Sources and Distribution Systems § 129.360 Semiconductor-rectifier systems. (a) Each semiconductor-rectifier system must have an adequate heat-removal system to prevent overheating. (b) If a...

46 CFR 129.360 - Semiconductor-rectifier systems.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 4 2010-10-01 2010-10-01 false Semiconductor-rectifier systems. 129.360 Section 129.360... INSTALLATIONS Power Sources and Distribution Systems § 129.360 Semiconductor-rectifier systems. (a) Each semiconductor-rectifier system must have an adequate heat-removal system to prevent overheating. (b) If a...

Presence and Potential Distribution of Aedes albopictus and Aedes japonicus japonicus (Diptera: Culicidae) in Slovenia.

PubMed

Kalan, Katja; Ivovic, Vladimir; Glasnovic, Peter; Buzan, Elena

2017-11-07

In Slovenia, two invasive mosquito species are present, Aedes albopictus (Skuse, 1895) (Diptera: Culicidae) and Aedes japonicus (Theobald, 1901) (Diptera: Culicidae). In this study, we examined their actual distribution and suitable habitats for new colonizations. Data from survey of species presence in 2013 and 2015, bioclimatic variables and altitude were used for the construction of predictive maps. We produced various models in Maxent software and tested two bioclimatic variable sets, WorldClim and CHELSA. For the variable selection of A. albopictus modeling we used statistical and expert knowledge-based approach, whereas for A. j. japonicus we used only a statistically based approach. The best performing models for both species were chosen according to AIC score-based evaluation. In 2 yr of sampling, A. albopictus was largely confined to the western half of Slovenia, whereas A. j. japonicus spread significantly and can be considered as an established species in a large part of the country. Comparison of models with WorldClim and CHELSA variables for both species showed models with CHELSA variables as a better tool for prediction. Finally, we validated the models performance in predicting distribution of species according to collected field data. Our study confirms that both species are co-occurring and are sympatric in a large part of the country area. The tested models could be used for future prevention of invasive mosquitoes spreading in other countries with similar bioclimatic conditions. © The Authors 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

Genome-wide identification of nuclear receptor (NR) superfamily genes in the copepod Tigriopus japonicus.

PubMed

Hwang, Dae-Sik; Lee, Bo-Young; Kim, Hui-Su; Lee, Min Chul; Kyung, Do-Hyun; Om, Ae-Son; Rhee, Jae-Sung; Lee, Jae-Seong

2014-11-18

Nuclear receptors (NRs) are a large superfamily of proteins defined by a DNA-binding domain (DBD) and a ligand-binding domain (LBD). They function as transcriptional regulators to control expression of genes involved in development, homeostasis, and metabolism. The number of NRs differs from species to species, because of gene duplications and/or lineage-specific gene losses during metazoan evolution. Many NRs in arthropods interact with the ecdysteroid hormone and are involved in ecdysone-mediated signaling in arthropods. The nuclear receptor superfamily complement has been reported in several arthropods, including crustaceans, but not in copepods. We identified the entire NR repertoire of the copepod Tigriopus japonicus, which is an important marine model species for ecotoxicology and environmental genomics. Using whole genome and transcriptome sequences, we identified a total of 31 nuclear receptors in the genome of T. japonicus. Nomenclature of the nuclear receptors was determined based on the sequence similarities of the DNA-binding domain (DBD) and ligand-binding domain (LBD). The 7 subfamilies of NRs separate into five major clades (subfamilies NR1, NR2, NR3, NR4, and NR5/6). Although the repertoire of NR members in, T. japonicus was similar to that reported for other arthropods, there was an expansion of the NR1 subfamily in Tigriopus japonicus. The twelve unique nuclear receptors identified in T. japonicus are members of NR1L. This expansion may be a unique lineage-specific feature of crustaceans. Interestingly, E78 and HR83, which are present in other arthropods, were absent from the genomes of T. japonicus and two congeneric copepod species (T. japonicus and Tigriopus californicus), suggesting copepod lineage-specific gene loss. We identified all NR receptors present in the copepod, T. japonicus. Knowledge of the copepod nuclear receptor repertoire will contribute to a better understanding of copepod- and crustacean-specific NR evolution.

46 CFR 129.320 - Generators and motors.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 4 2010-10-01 2010-10-01 false Generators and motors. 129.320 Section 129.320 Shipping... INSTALLATIONS Power Sources and Distribution Systems § 129.320 Generators and motors. (a) Each generator and... contact with low-lying vapors. (b) Each generator and motor must be designed for an ambient temperature of...

Occurrence and Spread of the Invasive Asian Bush Mosquito Aedes japonicus japonicus (Diptera: Culicidae) in West and North Germany since Detection in 2012 and 2013, Respectively.

PubMed

Kampen, Helge; Kuhlisch, Cornelius; Fröhlich, Andreas; Scheuch, Dorothee E; Walther, Doreen

2016-01-01

The invasive Asian bush mosquito Aedes japonicus japonicus was first recognised as established in Germany in 2008. In addition to the first known and quickly expanding population in the southwestern part of the country, three separate populations were discovered in West, North and southeastern Germany in 2012, 2013 and 2015, respectively, by means of the 'Mueckenatlas', a German instrument of passive mosquito surveillance. Since the first findings of mosquito specimens in West and North Germany, these regions were checked annually for continuing colonisation and spread of the species. Both affected areas were covered by a virtual 10x10km2 grid pattern in the cells of which cemeteries were screened for immature stages of the mosquito. The cells were considered populated as soon as larvae or pupae were detected, whereas they were classified as negative when no mosquito stages were found in the cemeteries of at least three different towns or villages. Presence was also recorded when Ae. j. japonicus adults were submitted to the 'Mueckenatlas' from the respective cell or when there was evidence of local occurrence in localities other than cemeteries. Based on this approach, a significant expansion of the populated area was documented in West Germany since the first detection of Ae. j. japonicus in 2012 (increase in positive grid cells by more than 400%), while the North German population appears not to be expanding so far (reduction of positive grid cells by ca. 30% since 2013). As Ae. j. japonicus finds suitable climatic and ecological conditions in Germany, the differential expansion of the two populations might be attributed to the West German population being older and thus more firmly established than the closely related but younger North German population that might still be in its founder phase. However, geographic spread of all German populations in the future is anticipated. Continuous surveillance is recommended, as Ae. j. japonicus is a competent vector of

Interspecific Larval Competition Between Aedes albopictus and Aedes japonicus (Diptera: Culicidae) in Northern Virginia

PubMed Central

Armistead, J. S.; Arias, J. R.; Nishimura, N.; Lounibos, L. P.

2008-01-01

Aedes albopictus (Skuse) and Aedes japonicus (Theobald) are two of the most recent and widespread invasive mosquito species to have become established in the United States. The two species co-occur in water-filled artificial containers, where crowding and limiting resources are likely to promote inter- or intraspecific larval competition. The performance of northern Virginia populations of Ae. japonicus and Ae. albopictus competing as larvae under field conditions was evaluated. Per capita rates of population increase for each species were estimated, and the effects of species composition and larval density were determined. In water-containing cups provided with oak leaves, Ae. albopictus larvae exhibited a competitive advantage over Ae. japonicus as a consequence of higher survivorship, shorter developmental time, and a significantly higher estimated population growth rate under conditions of interspecific competition. Intraspecific competition constrained population performance of Ae. albopictus significantly more than competition with Ae. japonicus. In the context of the Lotka-Volterra model of competition, these findings suggest competitive exclusion of Ae. japonicus in those habitats where this species co-occurs with Ae. albopictus. PMID:18714861

Interspecific larval competition between Aedes albopictus and Aedes japonicus (Diptera: Culicidae) in northern Virginia.

PubMed

Armistead, J S; Arias, J R; Nishimura, N; Lounibos, L P

2008-07-01

Aedes albopictus (Skuse) and Aedes japonicus (Theobald) are two of the most recent and widespread invasive mosquito species to have become established in the United States. The two species co-occur in water-filled artificial containers, where crowding and limiting resources are likely to promote inter- or intraspecific larval competition. The performance of northern Virginia populations of Ae. japonicus and Ae. albopictus competing as larvae under field conditions was evaluated. Per capita rates of population increase for each species were estimated, and the effects of species composition and larval density were determined. In water-containing cups provided with oak leaves, Ae. albopictus larvae exhibited a competitive advantage over Ae. japonicus as a consequence of higher survivorship, shorter developmental time, and a significantly higher estimated population growth rate under conditions of interspecific competition. Intraspecific competition constrained population performance of Ae. albopictus significantly more than competition with Ae. japonicus. In the context of the Lotka-Volterra model of competition, these findings suggest competitive exclusion of Ae. japonicus in those habitats where this species co-occurs with Ae. albopictus.

Proteomic investigation of the secretome of Cellvibrio japonicus during growth on chitin.

PubMed

Tuveng, Tina Rise; Arntzen, Magnus Øverlie; Bengtsson, Oskar; Gardner, Jeffrey G; Vaaje-Kolstad, Gustav; Eijsink, Vincent G H

2016-07-01

Studies of the secretomes of microbes grown on insoluble substrates are important for the discovery of novel proteins involved in biomass conversion. However, data in literature and this study indicate that secretome samples tend to be contaminated with cytoplasmic proteins. We have examined the secretome of the Gram-negative soil bacterium Cellvibrio japonicus using a simple plate-based culturing technique that yields samples with high fractions (60-75%) of proteins that are predicted to be secreted. By combining this approach with label-free quantification using the MaxLFQ algorithm, we have mapped and quantified proteins secreted by C. japonicus during growth on α- and β-chitin. Hierarchical clustering of the detected protein quantities revealed groups of up-regulated proteins that include all five putative C. japonicus chitinases as well as a chitin-specific lytic polysaccharide monooxygenase (CjLPMO10A). A small set of secreted proteins were co-regulated with known chitin-specific enzymes, including several with unknown catalytic functions. These proteins provide interesting targets for further studies aimed at unraveling the enzymatic machineries used by C. japonicus for recalcitrant polysaccharide degradation. Studies of chitin degradation indicated that C. japonicus indeed produces an efficient chitinolytic enzyme cocktail. All MS data have been deposited in the ProteomeXchange with the dataset identifier PXD002843 (http://proteomecentral.proteomexchange.org/dataset/PXD002843). © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Amino acid specificity of fibers of the facial/trigeminal complex innervating the maxillary barbel in the Japanese sea catfish, Plotosus japonicus.

PubMed

Caprio, John; Shimohara, Mami; Marui, Takayuki; Kohbara, Jun; Harada, Shuitsu; Kiyohara, Sadao

2015-12-01

The Japanese sea catfish, Plotosus japonicus, possesses taste and solitary chemoreceptor cells (SCCs) located on the external body surface that detect specific water-soluble substances. Here, we identify two major fiber types of the facial/trigeminal complex that transmit amino acid information to the medulla. Both single and few fiber preparations respond to amino acid stimulation in the 0.1 μM to mM range. One fiber type responds best to glycine and l-alanine (i.e. Gly/Ala fibers) whereas the other fiber type is best stimulated by l-proline and glycine betaine (hereafter referred to only as betaine) (i.e. Pro/Bet fibers). We demonstrate that betaine, which does not alter the pH of the seawater and therefore does not activate the animals' highly sensitive pH sensors (Caprio et al., Science 344:1154-1156, 2014), is sufficient to elicit appetitive food search behavior. We further show that the amino acid specificity of fibers of the facial/trigeminal complex in P. japonicus is different from that in Ariopsis felis (Michel and Caprio, J. Neurophysiol. 66:247-260, 1991; Michel et al., J. Comp. Physiol. A. 172:129-138, 1993), a representative member of the only other family (Ariidae) of extant marine catfishes. Copyright © 2015 Elsevier Inc. All rights reserved.

Evaluation of raw nepodin extraction from Rumex japonicus and R. obtusifolius and their DNA polymorphisms.

PubMed

Minami, Motoyasu; Mori, Takako; Yonezawa, Takayuki; Saito, Yukiko; Teruya, Toshiaki; Woo, Je-Tae

2018-01-01

Nepodin, found in the roots of Rumex japonicus Houtt. (Polygonaceae), inhibits osteoclast differentiation and has an antidiabetic effect. We propose nepodin as an ingredient of new functional foods or as a drug candidate for reducing the risk of reduced locomotion resulting from diseases such as osteoporosis. Although there are no previous reports of R. obtusifolius L., which is found throughout Japan, having roots containing nepodin, we found nepodin in the roots of this species. Therefore, R. obtusifolius as well as R. japonicus was considered a candidate raw material for nepodin extraction. We also discuss the suitability of R. japonicus and R. obtusifolius as sources of raw nepodin for cultivation on the Ryukyu Islands. In this study, all specimens on the Ryukyu Islands were identified as R. japonicus. Conversely, all specimens on mainland Japan were R. obtusifolius. The DNA sequence of the chloroplast trnL-trnF intergenic spacer region and partial nuclear internal transcribed spacer was consistent with the identification of R. japonicus and R. obtusifolius by morphological characteristics of the perianth segments. Therefore, to avoid erroneous identification and misuse of the plant species used for extraction of raw materials, it is preferable to develop DNA markers for these two regions. The content of nepodin varied from undetectable to 0.34% of the fresh weight (%FW) in R. japonicus and from undetectable to 0.21%FW in R. obtusifolius. From a pharmacological perspective, as plants that might be suitable as raw materials for nepodin extraction, it became clear that both R. japonicus and R. obtusifolius can be used with the same expected extraction efficiency. Based on our findings, R. obtusifolius could not be confirmed as inhabiting the Ryukyu Islands. For this reason, to conserve the endemic genetic characteristics of the Ryukyu Islands and to prevent genetic pollution by R. obtusifolius, only R. japonicus should be cultivated on the Ryukyu Islands.

Live 129I-129Xe dating

NASA Technical Reports Server (NTRS)

Marti, K.

1986-01-01

A technique of cosmic ray exposure age dating using cosmic ray produced I-129 and Xe-129 components is discussed. The live I-129 - Xe-129 method provides an ideal monitor for cosmic ray flux variations on the 10(7)y - 10(8)y time-scale. It is based on low-energy neutron reactions on Te, and these data, when coupled to those from other methods, may facilitate the detection of complex exposure histories.

Ontogenetic dietary shift in the larvae of Cybister japonicus (Coleoptera: Dytiscidae) in Japanese rice fields.

PubMed

Ohba, Shin-Ya

2009-06-01

A number of fragmentary reports suggest that the endangered diving beetle Cybister japonicus larvae feed on tadpoles, fish, and aquatic insects. However, no quantitative study on the feeding habits of C. japonicus larvae has been reported. In this study, field observations and rearing experiments were carried out to show the feeding ecology of C. japonicus larvae. Unlike previous commentaries, the first- and second-instar larvae of C. japonicus preyed on insects, mainly Odonata nymphs and Notonecta triguttata, irrespective of prey availability, but did not eat vertebrates such as tadpoles and fish in the field. On the contrary, the third-instar larvae fed on both insects and vertebrates. Rearing experiments showed that the number of Odonata nymphs consumed was significantly more than the number of tadpoles consumed by the first and second instars but third-instar larvae ate both the Odonata nymphs and tadpoles in the tadpole-Odonata nymph mixture experiment. The total body lengths of C. japonicus new adults in the Odonata nymph and tadpole-Odonata nymph mixture treatments were statistically equal. These results suggested that the first- and second-instar larvae of C. japonicus prey mainly on insects and do not eat vertebrate animals (insectivore), whereas the third-instar larvae fed on both insects and vertebrates (generalist).

[Studies on the chemical constituents from the flowers of Ophiopogon japonicus].

PubMed

Zhu, Yu-Hong; Zhao, Min; Ren, Lu; Tian, Di; Dou, Fang; Wang, Jun-Xian

2011-05-01

To study the chemical constituents from the flowers of Ophiopogon japonicus. Column chromatography and spectral analysis were used to isolate and identify the constituents. Eleven compounds were obtained and identified as beta-sitosterol (I), diosgenin (II), daucosterol (III), ophiopogonin C' (IV), dioscin (V), 7-dihy-droxy-6-methyl-3-(4'-hydroxybenzyl) chroman-4-one(VI), luteolin (VII), kaempferol-3-O-beta-D-glucopyranosides (VIII), kaempferol-3-O-(6"-tigloyl) -beta-D-glucopyranosides (IX), kaempferol-3-O-(6"-acetyl) -beta-D-glucopyranosides (X), glucose (XI). Eleven compounds are obtained from the flowers of O. japonicus for the first time. Compond VI is isolated as a simple substance compound of O. japonicus for the first time. Componds VII, VIII, IX and X are isolated from this genus for the first time.

Out of the bush: the Asian bush mosquito Aedes japonicus japonicus (Theobald, 1901) (Diptera, Culicidae) becomes invasive.

PubMed

Kampen, Helge; Werner, Doreen

2014-02-04

The Asian bush or rock pool mosquito Aedes japonicus japonicus is one of the most expansive culicid species of the world. Being native to East Asia, this species was detected out of its original distribution range for the first time in the early 1990s in New Zealand where it could not establish, though. In 1998, established populations were reported from the eastern US, most likely as a result of introductions several years earlier. After a massive spread the mosquito is now widely distributed in eastern North America including Canada and two US states on the western coast. In the year 2000, it was demonstrated for the first time in Europe, continental France, but could be eliminated. A population that had appeared in Belgium in 2002 was not controlled until 2012 as it did not propagate. In 2008, immature developmental stages were discovered in a large area in northern Switzerland and bordering parts of Germany. Subsequent studies in Germany showed a wide distribution and several populations of the mosquito in various federal states. Also in 2011, the species was found in southeastern Austria (Styria) and neighbouring Slovenia. In 2013, a population was detected in the Central Netherlands, specimens were collected in southern Alsace, France, and the complete northeastern part of Slovenia was found colonized, with specimens also present across borders in adjacent Croatia. Apparently, at the end of 2013 a total of six populations occurred in Europe although it is not clear whether all of them are completely isolated. Similarly, it is not known whether these populations go back to the same number of introductions. While entry ports and long-distance continental migration routes are also obscure, it is likely that the international used tyre trade is the most important mode of intercontinental transportation of the mosquito. Aedes j. japonicus does not only display an aggressive biting behaviour but is suspected to be a vector of various disease agents and to displace

46 CFR 129.315 - Power sources for OSVs.

Code of Federal Regulations, 2014 CFR

2014-10-01

... INSTALLATIONS Power Sources and Distribution Systems § 129.315 Power sources for OSVs. (a) The requirements of... 46 Shipping 4 2014-10-01 2014-10-01 false Power sources for OSVs. 129.315 Section 129.315 Shipping... subpart 111.10 of this chapter. (b) If a generator provides electrical power for any system identified as...

Improved potentials and Born-Oppenheimer corrections by new measurements of transitions of 129I2 and 127I 129I in the B3 Pi_O^+_u - X1 Σ^+_g band system

NASA Astrophysics Data System (ADS)

Salumbides, E. J.; Eikema, K. S. E.; Ubachs, W.; Hollenstein, U.; Knöckel, H.; Tiemann, E.

2008-04-01

The light from one single frequency cw laser was employed in a double saturation spectroscopy experiment to record high resolution spectra of 129I2 and 127I129I together with spectra of 127I2 which is used as a simultaneous frequency reference. Two separate saturation spectroscopy set ups were used. The frequencies of lines of 129I2 and 127I129I were determined with respect to lines of 127I2. More than 380 frequency differences between lines of 127I2 and of the other isotopomers have been included in the data set. By a direct potential fit a precise description of the potential energy curves of the B and the X state and of effective Born-Oppenheimer correction functions valid for all three isotopomers of I2 are given.

Spread of Aedes japonicus japonicus (Theobald, 1901) in Austria, 2011-2015, and first records of the subspecies for Hungary, 2012, and the principality of Liechtenstein, 2015.

PubMed

Seidel, Bernhard; Nowotny, Norbert; Bakonyi, Tamás; Allerberger, Franz; Schaffner, Francis

2016-06-24

The Asian bush mosquito, Aedes (Hulecoeteomyia) japonicus japonicus (Theobald, 1901) (Diptera: Culicidae), was first identified in Austria in August 2011 in the federal state of Styria at the border to Slovenia. Between 2011 and 2015 the spread of Ae. j. japonicus was monitored in southern, eastern and western Austrian provinces as well as in neighbouring countries by checking natural and man-made container habitats for the aquatic stages. The search concentrated around the most recent occurrence of Ae. j. japonicus and extended up to several kilometres until the subspecies could not be found anymore. Between May and July 2012 the distribution area of Ae. j. japonicus was found to be extended westwards into Carinthia, and eastwards towards the federal state of Burgenland. In August 2012, the subspecies was found in Hungary, representing the first record of an invasive mosquito species in this country. In 2013 its expansion was confirmed at several sites in Austria. Additionally, between April and July 2015, the subspecies was detected in all districts of the westernmost Austrian state Vorarlberg reaching the alpine Montafon valley at the end of October 2015, at all three examined sites in southern Bavaria bordering Vorarlberg, and in the adjacent Principality of Liechtenstein, for which it also represents the first record of an invasive mosquito species. One remarkable finding of the subspecies was located close to the city of Kufstein in the lower Inn valley of the Tyrol in September 2015, which is an isolated occurrence without spatial connection to any known established population. Our findings demonstrate the ongoing spread of Ae. j. japonicus towards all directions within Austria and beyond. Together with the absence of supposed natural barriers, e.g. high mountain chains, at the borders of the current subspecies' distribution area in south-eastern Austria, these findings suggest a further spread to the Austrian capital Vienna and the Hungarian tourist region

Accumulation and developmental toxicity of hexabromocyclododecanes (HBCDs) on the marine copepod Tigriopus japonicus.

PubMed

Shi, Dalin; Lv, Dongmei; Liu, Wanxin; Shen, Rong; Li, Dongmei; Hong, Haizheng

2017-01-01

The brominated flame retardants hexabromocyclododecanes (HBCDs) are ubiquitous environmental contaminants, widely distributed in aquatic systems including the marine environment and marine organisms. HBCDs are toxic to the development of both freshwater and marine fish. However, the impacts of HBCDs on marine invertebrates are not well known. In this study, the marine copepod, Tigriopus japonicus, was used to assess the bioaccumulation and developmental toxicity of technical HBCD (tHBCD) through water-borne exposure. The uptake rate constant of tHBCD by T. japonicus was high, which resulted in high bioaccumulation potential. The bioconcentration factors of tHBCD were 8.73 × 10 4 and 6.34 × 10 4  L kg -1 in T. japonicus, calculated using the kinetic and steady-state methods, respectively. Exposure of T. japonicus nauplii to tHBCD caused significant growth delay. The lowest-observable-effect-concentrations of tHBCD induced developmental delay were 30 and 8 μg L -1 for the F0 and F1 generations, respectively, which suggested that the F1 generation was more sensitive to tHBCD than the F0 generation and warranted multiple-generation toxicity tests for future studies. Furthermore, exposure of the adult copepods to tHBCD induced the transcription of oxidative stress response genes and apoptotic genes, e.g., SOD,CAT, GST, OGG1, P53 and Caspase-3. It was therefore speculated that tHBCD exposure induced the generation of reactive oxygen species in T. japonicus, which activated the oxidative stress defense genes and meanwhile resulted in oxidative DNA damage. The damaged DNA activated the transcription of p53 and triggered the caspase-mediated apoptosis pathway, which may be the reason for the tHBCD induced developmental delay in T. japonicus nauplii. Copyright © 2016 Elsevier Ltd. All rights reserved.

Dose- and time-dependent expression of aryl hydrocarbon receptor (AhR) and aryl hydrocarbon receptor nuclear translocator (ARNT) in PCB-, B[a]P-, and TBT-exposed intertidal copepod Tigriopus japonicus.

PubMed

Kim, Bo-Mi; Rhee, Jae-Sung; Hwang, Un-Ki; Seo, Jung Soo; Shin, Kyung-Hoon; Lee, Jae-Seong

2015-02-01

The aryl hydrocarbon receptor (AhR) and aryl hydrocarbon nuclear translocator (ARNT) genes from the copepod Tigriopus japonicus (Tj) were cloned to examine their potential functions in the invertebrate putative AhR-CYP signaling pathway. The amino acid sequences encoded by the Tj-AhR and Tj-ARNT genes showed high similarity to homologs of Daphnia and Drosophila, ranging from 68% and 70% similarity for the AhR genes to 56% for the ARNT genes. To determine whether Tj-AhR and Tj-ARNT are modulated by environmental pollutants, transcriptional expression of Tj-AhR and Tj-ARNT was analyzed in response to exposure to five concentrations of polychlorinated biphenyl (PCB 126) (control, 10, 50, 100, 500 μg L(-1)), benzo[a]pyrene (B[a]P) (control, 5, 10, 50, 100 μg L(-1)), and tributyltin (TBT) (control, 1, 5, 10, 20 μg L(-1)) 24h after exposure. A time-course experiment (0, 3, 6, 12, 24h) was performed to analyze mRNA expression patterns after exposure to PCB, B[a]P, and TBT. T. japonicus exhibited dose-dependent and time-dependent upregulation of Tj-AhR and Tj-ARNT in response to pollutant exposure, and the degree of expression was dependent on the pollutant, suggesting that pollutants such as PCB, B[a]P, and TBT modulate expression of Tj-AhR and Tj-ARNT genes in the putative AhR-CYP signaling pathway. Copyright © 2014 Elsevier Ltd. All rights reserved.

Apoptosis induction is involved in UVA-induced autolysis in sea cucumber Stichopus japonicus.

PubMed

Qi, Hang; Fu, Hui; Dong, Xiufang; Feng, Dingding; Li, Nan; Wen, Chengrong; Nakamura, Yoshimasa; Zhu, Beiwei

2016-05-01

Autolysis easily happens to sea cucumber (Stichopus japonicus, S. japonicus) for external stimulus like UV exposure causing heavy economic losses. Therefore, it is meaningful to reveal the mechanism of S. japonicas autolysis. In the present study, to examine the involvement of apoptosis induction in UVA-induced autolysis of S. japonicas, we investigated the biochemical events including the DNA fragmentation, caspase-3 activation, mitogen-activated protein kinases (MAPKs) phosphorylation and free radical formation. Substantial morphological changes such as intestine vomiting and dermatolysis were observed in S. japonicus during the incubation after 1-h UVA irradiation (10W/m(2)). The degradation of the structural proteins and enhancement of cathepsin L activity were also detected, suggesting the profound impact of proteolysis caused by the UVA irradiation even for 1h. Furthermore, the DNA fragmentation and specific activity of caspase-3 was increased up to 12h after UVA irradiation. The levels of phosphorylated p38 mitogen activated protein kinase (MAPK) and phosphorylated c-Jun.-N-terminal kinase (JNK) were significantly increased by the UVA irradiation for 1h. An electron spin resonance (ESR) analysis revealed that UVA enhanced the free radical formation in S. japonicas, even through we could not identify the attributed species. These results suggest that UVA-induced autolysis in S. japonicas at least partially involves the oxidative stress-sensitive apoptosis induction pathway. These data present a novel insight into the mechanisms of sea cucumber autolysis induced by external stress. Copyright © 2016 Elsevier B.V. All rights reserved.

The Hair Growth-Promoting Effect of Rumex japonicus Houtt. Extract

PubMed Central

Lee, Hyunkyoung; Kim, Na-Hyun; Yang, Hyeryeon; Bae, Seong Kyeong; Heo, Yunwi; Choudhary, Indu; Kwon, Young Chul; Byun, Jae Kuk; Yim, Hyeong Jun; Noh, Byung Seung; Heo, Jeong-Doo; Kim, Euikyung

2016-01-01

Rumex japonicus Houtt. is traditionally used as a medicinal plant to treat patients suffering from skin disease in Korea. However, the beneficial effect of Rumex japonicus Houtt. on hair growth has not been thoroughly examined. Therefore, the present study aims to investigate the hair growth-promoting effect of Rumex japonicus (RJ) Houtt. root extract using human dermal papilla cells (DPCs), HaCaT cells, and C57BL/6 mice model. RJ induced antiapoptotic and proliferative effects on DPCs and HaCaT cells by increasing Bcl-2/Bax ratio and activating cellular proliferation-related proteins, ERK and Akt. RJ also increased β-catenin via the inhibition of GSK-3β. In C57BL/6 mice model, RJ promoted the anagen induction and maintained its period. Immunohistochemistry analysis demonstrated that RJ upregulated Ki-67 and β-catenin expressions, suggesting that the hair growth effect of RJ may be mediated through the reinforcement of hair cell proliferation. These results provided important insights for the possible mechanism of action of RJ and its potential as therapeutic agent to promote hair growth. PMID:27974900

The host preference and impact of Argulus japonicus ectoparasite on cyprinids in Central Java, Indonesia

NASA Astrophysics Data System (ADS)

Kismiyati; Wulansari, P. D.; Dewi, N. N.

2018-04-01

The most widely cultivated freshwater fish are from Familia Cyprinidae, among others goldfish (Carassius auratus), koi (Cyprinus carpio) and comet goldfish (Carassius auratus auratus). One of the constraints of freshwater fish cultivation is ectoparasite infestation Argulus japonicus. Financial losses have been experienced by some farmers, caused by these ectoparasitic infestaions. This study was aimed to determine the impact of ectoparasite Argulus japonicus infestation on host (freshwater ornamental fish from Familia Cyprinidae), in order to find a preventive solution to treatment on the host. The results showed that prevalence of infested fish by Argulus japonicus were 57 % goldfish, 31 % comet fish and 65 % koi. Changes of histopathology on host were congestion, baoning degeneration, epithelium erosion and inflammatory cell infiltration. The image of infected leukocytes infested by Argulus japonicus were 8.5 % of lymphocytes, 4.7 % neurophils, 3.9 % monocytes, 1.45 % eosinophils and 0,17% basophils.

Larval mosquito habitat utilization and community dynamics of Aedes albopictus and Aedes japonicus (Diptera: Culicidae).

PubMed

Bartlett-Healy, Kristen; Unlu, Isik; Obenauer, Peter; Hughes, Tony; Healy, Sean; Crepeau, Taryn; Farajollahi, Ary; Kesavaraju, Banu; Fonseca, Dina; Schoeler, George; Gaugler, Randy; Strickman, Daniel

2012-07-01

Aedes albopictus (Skuse) and Ae. japonicus (Theobald) are important container-inhabiting mosquitoes that transmit disease agents, outcompete native species, and continue to expand their range in the United States. Both species deposit eggs in natural and artificial containers and thrive in peridomestic environments. The goal of our study was to examine the types and characteristics of containers that are most productive for these species in the northeastern United States. In total, 306 containers were sampled in urban, suburban, and rural areas of New Jersey. Multiple biotic and abiotic factors were recorded in an attempt to identify variables associated with the productivity of each species. Based on pupal abundance and density of container types, results showed that tires, trash cans, and planter dishes were the most important containers for Ae. albopictus, while planter dishes were the most important containers for Ae. japonicus. Container color (black and gray), material (rubber), and type (tires) were correlated with species presence for Ae. albopictus and Ae. japonicus. These factors may play a role in the selection of oviposition sites by female mosquitoes or in the survival of their progeny. Differences in species composition and abundance were detected between areas classified as urban, suburban, and rural. In urban and suburban areas, Ae. albopictus was more abundant in container habitats than Ae. japonicus; however, Ae. japonicus was more abundant in rural areas, and when water temperatures were below 14 degrees C. Our results suggest many variables can influence the presence of Ae. albopictus and Ae. japonicus in container habitats in northeastern United States.

22 CFR 129.7 - Prior approval (license).

Code of Federal Regulations, 2013 CFR

2013-04-01

... defense services (other than those that are arranged wholly within and destined exclusively for the North... Zealand, or the Republic of Korea (see §§ 129.6(b)(2) and 129.7(a)). (2) Brokering activities involving... under this subchapter, for or from any country not a member of the North Atlantic Treaty Organization...

Identification and expression characterization of WntA during intestinal regeneration in the sea cucumber Apostichopus japonicus.

PubMed

Li, Xiaoni; Sun, Lina; Yang, Hongsheng; Zhang, Libin; Miao, Ting; Xing, Lili; Huo, Da

2017-08-01

Wnt genes encode secreted glycoproteins that act as signaling molecules; these molecules direct cell proliferation, migration, differentiation and survival during animal development, maintenance of homeostasis and regeneration. At present, although the regeneration mechanism in Apostichopus japonicus has been studied, there is a little research on the Wnt signaling pathway in A. japonicus. To understand the potential role of the Wnt signaling pathway in A. japonicus, we cloned and sequenced the WntA gene in A. japonicus. Protein localization analysis showed that WntA protein was ubiquitously expressed in epidermal cells, the muscle and submucosa of the intestinal tissue. After stimulation and evisceration, the dynamic changes in expression of the WntA gene and protein showed that WntA was constitutively expressed during different stages of intestine regeneration in A. japonicus, with higher levels during the early wound healing stage and late lumen formation in the residual and nascent intestinal tissues, indicating its response to intestinal regeneration. Simultaneously, cell proliferation and apoptosis analysis showed that the patterns of cell proliferation were similar to the patterns of WntA protein expression during different intestinal regeneration stages in this organism. Taken together, these results suggested that WntA might participate in intestinal regeneration and may be connected with cell proliferation, apoptosis in different intestinal layers. This research could establish a basis for further examination of WntA functions in A. japonicus and Wnt genes in other echinoderms. Copyright © 2017 Elsevier Inc. All rights reserved.

Target-strength Measurements of Sandfish Arctoscopus japonicus

NASA Astrophysics Data System (ADS)

Yoon, Eun-A.; Lee, Kyounghoon; Oh, Wooseok; Choi, Junghwa; Hwang, Kangseok; Kang, Myounghee

2018-03-01

The goal of this study was to estimate the target strength (TS) of the sandfish Arctoscopus japonicus using in-situ and ex-situ methods with an echosounder. For the in-situ TS measurement, the survey was conducted by taking hydroacoustic measurements at 38 and 120 kHz and using a coastal gill net, in Goseong, in the northeastern sea of Korea in early December 2009. Ex-situ measurement of TS used live specimens and the tethering method, and was conducted at 120 kHz. The distribution of fork length (FL) was bimodal: 14.6-19.8 cm (n = 241 individuals, mean = 17.0 cm) for males and 16.3-24.5 cm (n = 105 individuals, mean = 19.6 cm) for females. The in-situ TS ranged from -79.8 to -59.1 dB (mean = -74.3 dB for males and -64.1 dB for females) at 38 kHz and -79.9 to -56.2 dB (mean = -74.3 dB for males and -64.1 dB for females) at 120 kHz. The mean TS of females was approximately 10 dB higher than that of males at each dominant frequency. The female ex-situ TS values ranged from 68.5 to -54.6 dB, and those of males was from -67.7 to -59.3 dB. The mean TS value for females was 2.9 dB higher than that of males. These results may be used in echo-integration surveys of sandfish to estimate their abundance and seasonal distribution.

Community Ecology of Container Mosquitoes (Diptera: Culicidae) in Virginia Following Invasion by Aedes japonicus

PubMed Central

ARMISTEAD, JENNIFER S.; NISHIMURA, NAOYA; ARIAS, JORGE R.; LOUNIBOS, L. PHILIP

2012-01-01

The success of an invasive species in a new region depends on its interactions with ecologically similar resident species. Invasions by disease vector mosquitoes are important as they may have ecological and epidemiological consequences. Potential interactions of a recent invasive mosquito, Aedes japonicus Theobald, with resident species in Virginia were evaluated by sampling larvae from containers and trapping adults. Distinct species compositions were observed for artificial containers and rock pools, with Ae. albopictus most abundant in the former and Ae. japonicus in the latter. However, these two species were found to co-occur in 21.2% of containers sampled. Among the six mosquito species most common in containers from May through September, 2006, only interspecific associations of Ae.japonicus with Aedesalbopictus(Skuse) and Aedestriseriatus(Say) were significant, and both were negative. In addition to differences in habitat preference, mean crowding estimates suggest that interspecific repulsion may contribute to the significant negative associations observed between these species. High relative abundances of late instars and pupae of Ae. japonicus seem to provide this species with a mechanism of evading competition with Ae. albopictus, facilitating their coexistence in artificial containers. Although annual fluctuations were observed, trends in adult populations over a 6-yr period provide no evidence of declines. In summary, this survey of diverse container types and all life stages provided only limited evidence for competitive displacements or reductions of resident container species by Ae. japonicus, as observed elsewhere in its invasive range. PMID:23270159

Change of digestive physiology in sea cucumber Apostichopus japonicus (Selenka) induced by corn kernels meal and soybean meal in diets

NASA Astrophysics Data System (ADS)

Yu, Haibo; Gao, Qinfeng; Dong, Shuanglin; Hou, Yiran; Wen, Bin

2016-08-01

The present study was conducted to determine the change of digestive physiology in sea cucumber Apostichopus japonicus (Selenka) induced by corn kernels meal and soybean meal in diets. Four experimental diets were tested, in which Sargassum thunbergii was proportionally replaced by the mixture of corn kernels meal and soybean meal. The growth performance, body composition and intestinal digestive enzyme activities in A. japonicus fed these 4 diets were examined. Results showed that the sea cucumber exhibited the maximum growth rate when 20% of S. thunbergii in the diet was replaced by corn kernels meal and soybean meal, while 40% of S. thunbergii in the diet can be replaced by the mixture of corn kernels meal and soybean meal without adversely affecting growth performance of A. japonicus. The activities of intestinal trypsin and amylase in A. japonicus can be significantly altered by corn kernels meal and soybean meal in diets. Trypsin activity in the intestine of A. japonicus significantly increased in the treatment groups compared to the control, suggesting that the supplement of corn kernels meal and soybean meal in the diets might increase the intestinal trypsin activity of A. japonicus. However, amylase activity in the intestine of A. japonicus remarkably decreased with the increasing replacement level of S. thunbergii by the mixture of corn kernels meal and soybean meal, suggesting that supplement of corn kernels meal and soybean meal in the diets might decrease the intestinal amylase activity of A. japonicus.

Aedes albopictus and Aedes japonicus - two invasive mosquito species with different temperature niches in Europe.

PubMed

Cunze, Sarah; Koch, Lisa K; Kochmann, Judith; Klimpel, Sven

2016-11-04

Aedes albopictus and Ae. japonicus are two of the most widespread invasive mosquito species that have recently become established in western Europe. Both species are associated with the transmission of a number of serious diseases and are projected to continue their spread in Europe. In the present study, we modelled the habitat suitability for both species under current and future climatic conditions by means of an Ensemble forecasting approach. We additionally compared the modelled MAXENT niches of Ae. albopictus and Ae. japonicus regarding temperature and precipitation requirements. Both species were modelled to find suitable habitat conditions in distinct areas within Europe: Ae. albopictus within the Mediterranean regions in southern Europe, Ae. japonicus within the more temperate regions of central Europe. Only in few regions, suitable habitat conditions were projected to overlap for both species. Whereas Ae. albopictus is projected to be generally promoted by climate change in Europe, the area modelled to be climatically suitable for Ae. japonicus is projected to decrease under climate change. This projection of range reduction under climate change relies on the assumption that Ae. japonicus is not able to adapt to warmer climatic conditions. The modelled MAXENT temperature niches of Ae. japonicus were found to be narrower with an optimum at lower temperatures compared to the niches of Ae. albopictus. Species distribution models identifying areas with high habitat suitability can help improving monitoring programmes for invasive species currently in place. However, as mosquito species are known to be able to adapt to new environmental conditions within the invasion range quickly, niche evolution of invasive mosquito species should be closely followed upon in future studies.

Trissolcus japonicus (Ashmead) emerges in North America

USDA-ARS?s Scientific Manuscript database

Trissolcus japonicus (Ashmead) is an Asian egg parasitoid of the brown marmorated stink bug, Halyomorpha halys (Stål). It has been under study in U.S. quarantine facilities since 2007 to evaluate its efficacy as a candidate classical biological control agent and its host specificity with regard to t...

Sweetener preference of C57BL/6ByJ and 129P3/J mice

PubMed Central

Bachmanov, Alexander A.; Tordoff, Michael G.; Beauchamp, Gary K.

2013-01-01

Previous studies have shown large differences in taste responses to several sweeteners between mice from the C57BL/6ByJ (B6) and 129P3/J (129) inbred strains. The goal of this study was to compare behavioral responses of the B6 and 129 mice to a wider variety of sweeteners. Seventeen sweeteners were tested using two-bottle preference tests with water. Three main patterns of strain differences were evident. First, sucrose, maltose, saccharin, acesulfame, sucralose and SC-45647 were preferred by both strains, but the B6 mice had lower preference thresholds and higher solution intakes. Second, the amino acids D-phenylalanine, D-tryptophan, L-proline and glycine were highly preferred by the B6 mice, but not by the 129 mice. Third, glycyrrhizic acid, neohesperidin dihydrochalcone, thaumatin and cyclamate did not evoke strong preferences in either strain. Aspartame was neutral to all 129 mice and some B6 mice, but other B6 mice strongly preferred it. Thus, compared with the 129 mice, the B6 mice had higher preferences for sugars, sweet-tasting amino acids and several but not all non-caloric sweeteners. Glycyrrhizic acid, neohesperidin, thaumatin and cyclamate are not palatable to B6 or 129 mice. PMID:11555485

Cytochrome b gene reveals panmixia among Japanese Threadfin Bream, Nemipterus japonicus (Bloch, 1791) populations along the coasts of Peninsular Malaysia and provides evidence of a cryptic species.

PubMed

Lim, Hong-Chiun; Ahmad, Abu Talib; Nuruddin, Ahmad Adnan; Mohd Nor, Siti Azizah

2016-01-01

We evaluated genetic differentiation among ten presumed Japanese threadfin bream, Nemipterus japonicus populations along the coast of Peninsular Malaysia based on the partial sequence of the mitochondrial cytochrome b gene (982 bp). Genetic divergences (Kimura-2 parameter) ranged from 0.5% to 0.8% among nine of the ten populations while these nine populations were 4.4% to 4.6% diverged from the Kuala Besar population located at the Northeast coast. The constructed Neighbour Joining (NJ) phylogenetic trees based on haplotypes showed the Kuala Besar population forming an isolated cluster. The Analysis of Molecular Variance (AMOVA) of the ten populations a priori assigned into four regions, revealed that most of the variation occurred within population with a fairly low but significant level of regional differentiation (FST = 0.07, p < 0.05, FSC = 0.00, p > 0.05 and FCT = 0.07, p < 0.05) attributed to the Kuala Besar population. p Value after Bonferroni correction revealed that only pairwise FST values involving the Kuala Besar population with the other nine populations were significant. Thus, this study revealed that the N. japonicus populations off Peninsular Malaysia were panmictic. However, the Kuala Besar population, although morphologically identical was composed of a genetically discrete taxon from the rest. These findings are important contributions in formulating sustainable fishery management policies for this important fishery in Peninsular Malaysia.

Development of Dirofilaria immitis and Dirofilaria repens in Aedes japonicus and Aedes geniculatus.

PubMed

Silaghi, Cornelia; Beck, Relja; Capelli, Gioia; Montarsi, Fabrizio; Mathis, Alexander

2017-02-20

The mosquito-borne filarial nematodes Dirofilaria immitis and Dirofilaria repens primarily affect dogs but also cats, causing heartworm disease or subcutaneous dirofilariosis, respectively, and both may also cause zoonotic diseases in humans. Several mosquito species have been reported as competent vectors for these nematodes, but no data are available for the invasive mosquito species Aedes japonicus (Theobald, 1901). The objective of this study was to describe the development of both D. immitis and D. repens under standardised experimental laboratory conditions in mosquitoes. For this purpose, both a laboratory strain and field-collected individuals of the invasive mosquito species Ae. japonicus and, for comparative purposes, a laboratory strain of Aedes geniculatus, a rare indigenous species sharing habitats with Ae. japonicus, and of the tropical species Aedes aegypti were used. Anticoagulated microfilariaemic blood was fed at a density of 3000 mf/ml to mosquitoes with a hemotek system. Blood-fed mosquitoes were incubated at 27 °C and 85% relative humidity, and specimens were dissected under the microscope at pre-set time points to observe developmental stages of both Dirofilaria species. Additionally, real-time PCRs were carried out in some microscopically negative samples to determine the infection rates. In field-collected Ae. japonicus infectious L3 larvae of both D. immitis and D. repens developed, rendering this mosquito species an efficient vector for both filarial species. Additionally, Ae. geniculatus was shown to be an equally efficient vector for both filarial species. Aedes japonicus mosquitoes from a laboratory colony were refractory to D. immitis but susceptible to D. repens, whereas Ae. aegypti was refractory to both filarial species. To our knowledge, Aedes japonicus was for the first time shown to be an efficient vector for both D. immitis and D. repens, indicating that this invasive and locally highly abundant species may contribute to a

Developmental retardation, reduced fecundity, and modulated expression of the defensome in the intertidal copepod Tigriopus japonicus exposed to BDE-47 and PFOS.

PubMed

Han, Jeonghoon; Won, Eun-Ji; Lee, Min-Chul; Seo, Jung Soo; Lee, Su-Jae; Lee, Jae-Seong

2015-08-01

2,2',4,4'-tetrabromodiphenyl ether (BDE-47) and perfluorooctane sulfonate (PFOS) are widely dispersed persistent organic pollutants (POPs) in the marine ecosystem. However, their toxic effects on marine organisms are still poorly understood. In this study, we investigated the effects of BDE-47 and PFOS on development and reproduction at the organismal level and reactive oxygen species (ROS) production and gene expression patterns of the defensome at the cellular level in the intertidal copepod Tigriopus japonicus. In copepods exposed to BDE-47 and PFOS, we observed developmental retardation and reduced fecundity, suggesting repercussions on in vivo endpoints through alterations to the normal molting and reproduction system of T. japonicus. BDE-47 and PFOS increased levels of ROS in T. japonicus in a concentration-dependent manner, indicating that POPs can induce oxidative stress through the generation of ROS. Additionally, transcript profiles of genes related to detoxification (e.g., CYPs), antioxidant functions (e.g., GST- sigma, catalase, MnSOD), apoptosis (e.g., p53, Rb), and cellular proliferation (e.g., PCNA) were modulated over 72h in response to BDE-47 (120μg/L) and PFOS (1000μg/L). These findings indicate that BDE-47 and PFOS can induce oxidative stress-mediated DNA damage repair systems with transcriptional regulation of detoxification, antioxidant, and apoptosis-related genes, resulting in developmental retardation and reduced fecundity in the copepod T. japonicus. Copyright © 2015 Elsevier B.V. All rights reserved.

Analysis of fatty acid composition of sea cucumber Apostichopus japonicus using multivariate statistics

NASA Astrophysics Data System (ADS)

Xu, Qinzeng; Gao, Fei; Xu, Qiang; Yang, Hongsheng

2014-11-01

Fatty acids (FAs) provide energy and also can be used to trace trophic relationships among organisms. Sea cucumber Apostichopus japonicus goes into a state of aestivation during warm summer months. We examined fatty acid profiles in aestivated and non-aestivated A. japonicus using multivariate analyses (PERMANOVA, MDS, ANOSIM, and SIMPER). The results indicate that the fatty acid profiles of aestivated and non-aestivated sea cucumbers differed significantly. The FAs that were produced by bacteria and brown kelp contributed the most to the differences in the fatty acid composition of aestivated and nonaestivated sea cucumbers. Aestivated sea cucumbers may synthesize FAs from heterotrophic bacteria during early aestivation, and long chain FAs such as eicosapentaenoic (EPA) and docosahexaenoic acid (DHA) that produced from intestinal degradation, are digested during deep aestivation. Specific changes in the fatty acid composition of A. japonicus during aestivation needs more detailed study in the future.

LncRNA TNRC6C-AS1 regulates UNC5B in thyroid cancer to influence cell proliferation, migration, and invasion as a competing endogenous RNA of miR-129-5p.

PubMed

Hou, Sen; Lin, Qiuyu; Guan, Feng; Lin, Chenghe

2018-06-12

To investigate the biological functions and regulatory mechanism of lncRNA TNRC6C-AS1 in thyroid cancer (TC). TNRC6C-AS1, miR-129-5p, and UNC5B expression levels were investigated by qRT-PCR and Western blot. CCK-8 assay was conducted to determine cell proliferation, while transwell assay was for inspection of cell migration and invasion. Through bioinformatic analysis, the interactions among TNRC6C-AS1, miR-129-5p, and UNC5B were predicted. Dual luciferase reporter gene assay and RNA pull-down assay confirmed the predicted target relationships. Tumor xenograft assay was applied to inspect the effect of TNRC6C-AS1 downregulation on TC development in vivo. TNRC6C-AS1 and UNC5B were overexpressed, while miR-129-5p was underexpressed in TC tissues and cells. TNRC6C-AS1/UNC5B downregulation and miR-129-5p overexpression could suppress proliferation, migration, and invasion of TC cells as well as inhibit tumorigenesis in vivo. MiR-129-5p targeted TNRC6C-AS1 and UNC5B in TC cells; and UNC5B expression was downregulated by knocking down TNRC6C-AS1, which competitively bound with miR-129-5p. Downregulation of TNRC6C-AS1 restrained TC development by knocking down UNC5B through upregulating the expression of miR-129-5p. © 2018 Wiley Periodicals, Inc.

Sustainability evaluation of different systems for sea cucumber ( Apostichopus japonicus) farming based on emergy theory

NASA Astrophysics Data System (ADS)

Wang, Guodong; Dong, Shuanglin; Tian, Xiangli; Gao, Qinfeng; Wang, Fang

2015-06-01

Emergy analysis is effective for analyzing ecological economic systems. However, the accuracy of the approach is affected by the diversity of economic level, meteorological and hydrological parameters in different regions. The present study evaluated the economic benefits, environmental impact, and sustainability of indoor, semi-intensive and extensive farming systems of sea cucumber ( Apostichopus japonicus) in the same region. The results showed that A. japonicus indoor farming system was high in input and output (yield) whereas pond extensive farming system was low in input and output. The output/input ratio of indoor farming system was lower than that of pond extensive farming system, and the output/input ratio of semi-intensive farming system fell in between them. The environmental loading ratio of A. japonicus extensive farming system was lower than that of indoor farming system. In addition, the emergy yield and emergy exchange ratios, and emergy sustainability and emergy indexes for sustainable development were higher in extensive farming system than those in indoor farming system. These results indicated that the current extensive farming system exerted fewer negative influences on the environment, made more efficient use of available resources, and met more sustainable development requirements than the indoor farming system. A. japonicus farming systems showed more emergy benefits than fish farming systems. The pond farming systems of A. japonicus exploited more free local environmental resources for production, caused less potential pressure on the local environment, and achieved higher sustainability than indoor farming system.

Draft genome of the sea cucumber Apostichopus japonicus and genetic polymorphism among color variants.

PubMed

Jo, Jihoon; Oh, Jooseong; Lee, Hyun-Gwan; Hong, Hyun-Hee; Lee, Sung-Gwon; Cheon, Seongmin; Kern, Elizabeth M A; Jin, Soyeong; Cho, Sung-Jin; Park, Joong-Ki; Park, Chungoo

2017-01-01

The Japanese sea cucumber (Apostichopus japonicus Selenka 1867) is an economically important species as a source of seafood and ingredient in traditional medicine. It is mainly found off the coasts of northeast Asia. Recently, substantial exploitation and widespread biotic diseases in A. japonicus have generated increasing conservation concern. However, the genomic knowledge base and resources available for researchers to use in managing this natural resource and to establish genetically based breeding systems for sea cucumber aquaculture are still in a nascent stage. A total of 312 Gb of raw sequences were generated using the Illumina HiSeq 2000 platform and assembled to a final size of 0.66 Gb, which is about 80.5% of the estimated genome size (0.82 Gb). We observed nucleotide-level heterozygosity within the assembled genome to be 0.986%. The resulting draft genome assembly comprising 132 607 scaffolds with an N50 value of 10.5 kb contains a total of 21 771 predicted protein-coding genes. We identified 6.6-14.5 million heterozygous single nucleotide polymorphisms in the assembled genome of the three natural color variants (green, red, and black), resulting in an estimated nucleotide diversity of 0.00146. We report the first draft genome of A. japonicus and provide a general overview of the genetic variation in the three major color variants of A. japonicus. These data will help provide a comprehensive view of the genetic, physiological, and evolutionary relationships among color variants in A. japonicus, and will be invaluable resources for sea cucumber genomic research. © The Author 2017. Published by Oxford University Press.

Gamma rays induce DNA damage and oxidative stress associated with impaired growth and reproduction in the copepod Tigriopus japonicus.

PubMed

Han, Jeonghoon; Won, Eun-Ji; Lee, Bo-Young; Hwang, Un-Ki; Kim, Il-Chan; Yim, Joung Han; Leung, Kenneth Mei Yee; Lee, Yong Sung; Lee, Jae-Seong

2014-07-01

the expression of antioxidant enzymes and proteins with chaperone-related functions, thereby significantly affecting life history parameters such as fecundity and molting in the copepod T. japonicus. Copyright © 2014 Elsevier B.V. All rights reserved.

Comparison of intestinal microbiota and activities of digestive and immune-related enzymes of sea cucumber Apostichopus japonicus in two habitats

NASA Astrophysics Data System (ADS)

Wang, Qi; Zhang, Xiumei; Chen, Muyan; Li, Wentao; Zhang, Peidong

2017-09-01

Sea cucumber Apostichopus japonicus stock enhancement by releasing hatchery-produced seeds is a management tool used to recover its population under natural environmental conditions. To assess the suitability of releasing sites, we examined the microbiota of the gut contents of A. japonicus from two populations (one in sandy-muddy seagrass beds and one in rocky intertidal reefs) and the microbiota in their surrounding sediments. The activities of digestive and immune-related enzymes in the A. japonicus were also examined. The results indicated that higher bacterial richness and Shannon diversity index were observed in all the seagrass-bed samples. There were significant differences in intestinal and sediment microorganisms between the two habitats, with a 2.87 times higher abundance of Firmicutes in the seagrass bed sediments than that in the reefs. Meanwhile, Bacteroidetes and Actinobacteria were significantly higher abundant in the gut content of A. japonicus from seagrass bed than those from the reefs. In addition, the seagrass-bed samples exhibited a relatively higher abundance of potential probiotics. Principal coordinates analysis and heatmap showed the bacterial communities were classified into two groups corresponding to the two habitat types. Moreover, compared to A. japonicus obtained from rocky intertidal habitat, those obtained from the seagrass bed showed higher lysozyme, superoxide dismutase and protease activities. Our results suggest that bacterial communities present in seagrass beds might enhance the digestive function and immunity of A. japonicus. Therefore, compared with the rocky intertidal reef, seagrass bed seems to be more beneficial for the survival of A. japonicus.

Demographic response of cutlassfish (Trichiurus japonicus and T. nanhaiensis) to fluctuating palaeo-climate and regional oceanographic conditions in the China seas.

PubMed

He, Lijun; Zhang, Aibing; Weese, David; Li, Shengfa; Li, Jiansheng; Zhang, Jing

2014-09-16

Glacial cycles of the Quaternary have heavily influenced the demographic history of various species. To test the evolutionary impact of palaeo-geologic and climatic events on the demographic history of marine taxa from the coastal Western Pacific, we investigated the population structure and demographic history of two economically important fish (Trichiurus japonicus and T. nanhaiensis) that inhabit the continental shelves of the East China and northern South China Seas using the mitochondrial cytochrome b sequences and Bayesian Skyline Plot analyses. A molecular rate of 2.03% per million years, calibrated to the earliest flooding of the East China Sea shelf (70-140 kya), revealed a strong correlation between population sizes and primary production. Furthermore, comparison of the demographic history of T. japonicus populations from the East China and South China Seas provided evidence of the postglacial development of the Changjiang (Yangtze River) Delta. In the South China Sea, interspecific comparisons between T. japonicus and T. nanhaiensis indicated possible evolutionary responses to changes in palaeo-productivity that were influenced by East Asian winter monsoons. This study not only provides insight into the demographic history of cutlassfish but also reveals potential clues regarding the historic productivity and regional oceanographic conditions of the Western Pacific marginal seas.

Demographic response of cutlassfish (Trichiurus japonicus and T. nanhaiensis) to fluctuating palaeo-climate and regional oceanographic conditions in the China seas

PubMed Central

He, Lijun; Zhang, Aibing; Weese, David; Li, Shengfa; Li, Jiansheng; Zhang, Jing

2014-01-01

Glacial cycles of the Quaternary have heavily influenced the demographic history of various species. To test the evolutionary impact of palaeo-geologic and climatic events on the demographic history of marine taxa from the coastal Western Pacific, we investigated the population structure and demographic history of two economically important fish (Trichiurus japonicus and T. nanhaiensis) that inhabit the continental shelves of the East China and northern South China Seas using the mitochondrial cytochrome b sequences and Bayesian Skyline Plot analyses. A molecular rate of 2.03% per million years, calibrated to the earliest flooding of the East China Sea shelf (70–140 kya), revealed a strong correlation between population sizes and primary production. Furthermore, comparison of the demographic history of T. japonicus populations from the East China and South China Seas provided evidence of the postglacial development of the Changjiang (Yangtze River) Delta. In the South China Sea, interspecific comparisons between T. japonicus and T. nanhaiensis indicated possible evolutionary responses to changes in palaeo-productivity that were influenced by East Asian winter monsoons. This study not only provides insight into the demographic history of cutlassfish but also reveals potential clues regarding the historic productivity and regional oceanographic conditions of the Western Pacific marginal seas. PMID:25223336

Comparative expression analysis of immune-related factors in the sea cucumber Apostichopus japonicus.

PubMed

Jiang, Jingwei; Zhou, Zunchun; Dong, Ying; Zhao, Zelong; Sun, Hongjuan; Wang, Bai; Jiang, Bei; Chen, Zhong; Gao, Shan

2018-01-01

In order to preliminarily explore the joint involvement of different immune-related factors during the same immune process in Apostichopus japonicus, the transcriptional expression of Cu/Zn superoxide dismutase (Cu/Zn-SOD), catalase (CAT), c-type lysozyme (c-LYZ), i-type lysozyme (i-LYZ), cathepsin D, melanotransferrin (MTF), Toll, c-type lectin (c-LCT) and complement 3 (C3) during the development from fertilized eggs to juveniles and after challenging the juveniles with Vibrio splendidus, Pseudoalteromonas nigrifaciens, Shewanella baltica and Bacillus cereus, respectively, was measured using the method of quantitative real-time PCR (qRT-PCR), and then the correlations among different immune-related factors were analyzed. The results showed that the selected immune-related factors were expressed at all of the determined developmental stages and significantly up-regulated at doliolaria stage, suggesting the selected factors are indispensable immune components and the immune system might be broadly activated at doliolaria stage in A. japonicus. After challenged with four pathogenic bacteria, Cu/Zn-SOD, CAT, i-LYZ, cathepsin D, MTF, Toll, C3 were all significantly down-regulated at 4 h, indicating that some components of A. japonicus immune system might be inhibited at the beginning of pathogenic bacteria invasion. The immune-responsive analysis also showed that the significant regulation in Toll after challenged with four tested bacteria, that in MTF after challenged with S. baltica and that in C3 after challenged with P. nigrifaciens were all minus, suggesting Toll, MTF and C3 are probably the primary targets of pathogenic bacteria attack. Furthermore, the correlation analysis indicated that, all of the selected immune-related factors except cathepsin D might be in the same immune regulatory network during A. japonicus development, while all of the selected immune-related factors except c-LYZ might be in the same responsive regulatory network after challenged with

Influence of flow velocity on motor behavior of sea cucumber Apostichopus japonicus.

PubMed

Pan, Yang; Zhang, Libin; Lin, Chenggang; Sun, Jiamin; Kan, Rentao; Yang, Hongsheng

2015-05-15

The influence of flow velocity on the motor behavior of the sea cucumber, Apostichopus japonicus was investigated in the laboratory. Cameras were used to record sea cucumber movements and behavior analysis software was used to measure the distance traveled, time spent, upstream or downstream of the start position and the speed of movements. In general, the mean velocity of A. japonicus was below 0.7mms(-1). The maximum velocity recorded for all the sea cucumbers tested was for a large individual (89.25±17.11g), at a flow rate of 4.6±0.5cms(-1). Medium sized (19.68±5.53g) and large individuals moved significantly faster than small individuals (2.65±1.24g) at the same flow rate. A. japonicus moved significantly faster when there was a moderate current (4.6±0.5cms(-1) and 14.7±0.3cms(-1)), compared with the fast flow rate (29.3±3.7cms(-1)) and when there was no flow (0cms(-1)). Sea cucumbers did not show positive rheotaxis in general, but did move in a downstream direction at faster current speeds. Large, medium and small sized individuals moved downstream at the fastest current speed tested, 29.3±3.7cms(-1). When there was no water flow, sea cucumbers tended to move in an irregular pattern. The movement patterns show that the sea cucumber, A. japonicus can move across the direction of flow, and can move both upstream and downstream along the direction of flow. Copyright © 2015. Published by Elsevier Inc.

Habitat suitability index model of the sea cucumber Apostichopus japonicus (Selenka): A case study of Shandong Peninsula, China.

PubMed

Zhang, Zhipeng; Zhou, Jian; Song, Jingjing; Wang, Qixiang; Liu, Hongjun; Tang, Xuexi

2017-09-15

A habitat suitability index (HSI) model for the sea cucumber Apostichopus japonicus (Selenka) was established in the present study. Based on geographic information systems, the HSI model was used to identify potential sites around the Shandong Peninsula suitable for restoration of immature (<25g) and mature (>25g) A. japonicus. Six habitat factors were used as input variables for the HSI model: sediment classification, water temperature, salinity, water depth, pH and dissolved oxygen. The weighting of each habitat factor was defined through the Delphi method. Sediment classification was the most important condition affecting the HSI of A. japonicus in the different study areas, while water temperature was the most important condition in different seasons. The HSI of Western Laizhou Bay was relatively low, meaning the site was not suitable for aquaculture-based restoration of A. japonicus. In contrast, Xiaoheishan Island, Rongcheng Bay and Qingdao were preferable sites, suitable as habitats for restoration efforts. Copyright © 2017 Elsevier Ltd. All rights reserved.

Comprehensive functional characterization of the Glycoside Hydrolase Family 3 enzymes from Cellvibrio japonicus reveals unique metabolic roles in biomass saccharification

DOE Office of Scientific and Technical Information (OSTI.GOV)

Nelson, Cassandra E.; Attia, Mohamed A.; Rogowski, Artur

Here, lignocellulose degradation is central to the carbon cycle and renewable biotechnologies. The xyloglucan (XyG), β(1!3)/β(1!4) mixed-linkage glucan (MLG), and β(1!3) glucan components of lignocellulose represent significant carbohydrate energy sources for saprophytic microorganisms. The bacterium Cellvibrio japonicus has a robust capacity for plant polysaccharide degradation, due to a genome encoding a large contingent of Carbohydrate-Active Enzymes (CAZymes), many of whose specific functions remain unknown. Using a comprehensive genetic and biochemical approach we have delineated the physiological roles of the four C. japonicus Glycoside Hydrolase Family 3 (GH3) members on diverse β-glucans. Despite high protein sequence similarity and partially overlapping activitymore » profiles on disaccharides, these β-glucosidases are not functionally equivalent. Bgl3A has a major role in MLG and sophorose utilization, and supports β(1!3) glucan utilization, while Bgl3B underpins cellulose utilization and supports MLG utilization. Bgl3C drives β(1!3) glucan utilization. Finally, Bgl3D is the crucial β-glucosidase for XyG utilization. This study not only sheds the light on the metabolic machinery of C. japonicus, but also expands the repertoire of characterized CAZymes for future deployment in biotechnological applications. In particular, the precise functional analysis provided here serves as a reference for informed bioinformatics on the genomes of other Cellvibrio and related species.« less

Comprehensive functional characterization of the Glycoside Hydrolase Family 3 enzymes from Cellvibrio japonicus reveals unique metabolic roles in biomass saccharification

DOE PAGES

Nelson, Cassandra E.; Attia, Mohamed A.; Rogowski, Artur; ...

2017-10-20

Here, lignocellulose degradation is central to the carbon cycle and renewable biotechnologies. The xyloglucan (XyG), β(1!3)/β(1!4) mixed-linkage glucan (MLG), and β(1!3) glucan components of lignocellulose represent significant carbohydrate energy sources for saprophytic microorganisms. The bacterium Cellvibrio japonicus has a robust capacity for plant polysaccharide degradation, due to a genome encoding a large contingent of Carbohydrate-Active Enzymes (CAZymes), many of whose specific functions remain unknown. Using a comprehensive genetic and biochemical approach we have delineated the physiological roles of the four C. japonicus Glycoside Hydrolase Family 3 (GH3) members on diverse β-glucans. Despite high protein sequence similarity and partially overlapping activitymore » profiles on disaccharides, these β-glucosidases are not functionally equivalent. Bgl3A has a major role in MLG and sophorose utilization, and supports β(1!3) glucan utilization, while Bgl3B underpins cellulose utilization and supports MLG utilization. Bgl3C drives β(1!3) glucan utilization. Finally, Bgl3D is the crucial β-glucosidase for XyG utilization. This study not only sheds the light on the metabolic machinery of C. japonicus, but also expands the repertoire of characterized CAZymes for future deployment in biotechnological applications. In particular, the precise functional analysis provided here serves as a reference for informed bioinformatics on the genomes of other Cellvibrio and related species.« less

Lung morphometry using hyperpolarized 129 Xe multi-b diffusion MRI with compressed sensing in healthy subjects and patients with COPD.

PubMed

Zhang, Huiting; Xie, Junshuai; Xiao, Sa; Zhao, Xiuchao; Zhang, Ming; Shi, Lei; Wang, Ke; Wu, Guangyao; Sun, Xianping; Ye, Chaohui; Zhou, Xin

2018-05-04

To demonstrate the feasibility of compressed sensing (CS) to accelerate the acquisition of hyperpolarized (HP) 129 Xe multi-b diffusion MRI for quantitative assessments of lung microstructural morphometry. Six healthy subjects and six chronic obstructive pulmonary disease (COPD) subjects underwent HP 129 Xe multi-b diffusion MRI (b = 0, 10, 20, 30, and 40 s/cm 2 ). First, a fully sampled (FS) acquisition of HP 129 Xe multi-b diffusion MRI was conducted in one healthy subject. The acquired FS dataset was retrospectively undersampled in the phase encoding direction, and an optimal twofold undersampled pattern was then obtained by minimizing mean absolute error (MAE) between retrospective CS (rCS) and FS MR images. Next, the FS and CS acquisitions during separate breath holds were performed on five healthy subjects (including the above one). Additionally, the FS and CS synchronous acquisitions during a single breath hold were performed on the sixth healthy subject and one COPD subject. However, only CS acquisitions were conducted in the rest of the five COPD subjects. Finally, all the acquired FS, rCS and CS MR images were used to obtain morphometric parameters, including acinar duct radius (R), acinar lumen radius (r), alveolar sleeve depth (h), mean linear intercept (L m ), and surface-to-volume ratio (SVR). The Wilcoxon signed-rank test and the Bland-Altman plot were employed to assess the fidelity of the CS reconstruction. Moreover, the t-test was used to demonstrate the effectiveness of the multi-b diffusion MRI with CS in clinical applications. The retrospective results demonstrated that there was no statistically significant difference between rCS and FS measurements using the Wilcoxon signed-rank test (P > 0.05). Good agreement between measurements obtained with the CS and FS acquisitions during separate breath holds was demonstrated in Bland-Altman plots of slice differences. Specifically, the mean biases of the R, r, h, L m , and SVR between the CS and

Anti-fatigue activity of sea cucumber peptides prepared from Stichopus japonicus in an endurance swimming rat model.

PubMed

Ye, Jing; Shen, Caihong; Huang, Yayan; Zhang, Xueqin; Xiao, Meitian

2017-10-01

Sea cucumber (Stichopus japonicus) is a well-known nutritious and luxurious seafood in Asia which has attracted increasing attention because of its nutrition and bioactivities in recent years. In this study, the anti-fatigue activity of sea cucumber peptides (SCP) prepared from S. japonicus was evaluated in a load-induced endurance swimming model. The SCP prepared in this study was mainly made up of low-molecular-weight peptides (<2 kDa). The analysis result of amino acid composition revealed that SCP was rich in glycine, glutamic acid and proline. The endurance capability of rats to fatigue was significantly improved by SCP treatment. Meanwhile, the remarkable alterations of energy metabolic markers, antioxidant enzymes, antioxidant capacity and oxidative stress biomarkers were normalized. Moreover, administration of SCP could modulate alterations of inflammatory cytokines and downregulate the overexpression of TRL4 and NF-κB. SCP has anti-fatigue activity and it exerted its anti-fatigue effect probably through normalizing energy metabolism as well as alleviating oxidative damage and inflammatory responses. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

Proteomic analysis reveals the important roles of alpha-5-collagen and ATP5β during skin ulceration syndrome progression of sea cucumber Apostichopus japonicus.

PubMed

Zhao, Zelong; Jiang, Jingwei; Pan, Yongjia; Sun, Hongjuan; Guan, Xiaoyan; Gao, Shan; Chen, Zhong; Dong, Ying; Zhou, Zunchun

2018-03-20

Apostichopus japonicus is one of the most important aquaculture species in China. Skin ulceration syndrome (SUS) of sea cucumber is a common and serious disease affected the development of A. japonicus culture industry. To better understand the response mechanisms of A. japonicus during SUS progression, the protein variations in the body wall of A. japonicus at different stages of SUS were investigated by a comparative proteomic approach based on isobaric tags for relative and absolute quantification. A total of 1449 proteins were identified from the samples at different SUS stages. Among these proteins, 145 proteins were differentially expressed in the SUS-related samples compared to those of healthy A. japonicus. These differentially expressed proteins involved a wide range of functions. Among these differentially expressed proteins, only two proteins, alpha-5-collagen and an unknown function protein, were differentially expressed during the whole progression of SUS compared with healthy A. japonicus. In addition, ATP synthase subunit beta (ATP5β) interacted with a variety of proteins with different functions during the SUS progression. These results implied that alpha-5-collagen and ATP5β could play important roles during the SUS progression of A. japonicus. Our study provided a new sight to understand the molecular responses of sea cucumber during the SUS progression and accumulated data for the prevention of SUS in sea cucumber aquaculture. The current study aimed to reveal how the body wall of Apostichopus japonicus response to skin ulceration syndrome (SUS). To the best of our knowledge, this is the first proteomic study analyzing the differences in protein profile of sea cucumber during the whole SUS progression. By analyzing the expression differences of the proteome via isobaric labeling-based quantitative proteomic, we identified some proteins which may play important roles during the SUS progression. According to the enrichment analyses of these

Seasonal changes in food uptake by the sea cucumber Apostichopus japonicus in a farm pond: Evidence from C and N stable isotopes

NASA Astrophysics Data System (ADS)

Sun, Zhenlong; Gao, Qinfeng; Dong, Shuanglin; Shin, Paul K. S.; Wang, Fang

2013-03-01

This study investigated the seasonal changes in carbon (C) and nitrogen (N) stable isotope values of several typical food sources of Apostichopus japonicus in a farm pond, including particulate organic matter (POM), macroalgae, benthic microalgae and animals such as nematode and copepod. The stable isotope technique was used to quantify relative contributions of various sources to the food uptake by A. japonicus. The results showed that significant changes occurred in the C and N stable isotope values of sea cucumber food sources due to the seasonality of micro- or macroalgae prosperity and the fluctuation of environmental conditions. The sea cucumber A. japonicus exhibited corresponding alterations in feeding strategy in response to the changes in food conditions. Calculation with a stable isotope mixing model showed that macroalgae was the principal food source for A. japonicus throughout the 1-yr investigation, with the relative contribution averaging 28.1%-63.2%. The relative contributions of other food sources such as copepod and nematode, POM, benthic microalgae to the total food uptake by sea cucumber averaged 22.6%-39.1%, 6.3% -22.2%, 2.8%-6.5%, and 2.8%-4.2%, respectively. Together these results indicated that the seasonal changes in food sources led to the obvious temporal differences in the relative contribution of various food sources utilized by A. japonicus. Such findings provide the basic scientific information for improving the aquaculture techniques of A. japonicus, particularly for optimizing the food environment of A. japonicus culture in farm ponds.

Identification of the geographical origins of sea cucumber (Apostichopus japonicus) in northern China by using stable isotope ratios and fatty acid profiles.

PubMed

Zhang, Xufeng; Liu, Yu; Li, Ying; Zhao, Xinda

2017-03-01

Geographic traceability is an important issue for food quality and safety control of seafood. In this study,δ 13 C and δ 15 N values, as well as fatty acid (FA) content of 133 samples of A. japonicus from seven sampling points in northern China Sea were determined to evaluate their applicability in the origin traceability of A. japonicus. Principal component analysis (PCA) and discriminant analysis (DA) were applied to different data sets in order to evaluate their performance in terms of classification or predictive ability. δ 13 C and δ 15 N values could effectively discriminate between different origins of A. japonicus. Significant differences in the FA compositions showed the effectiveness of FA composition as a tool for distinguishing between different origins of A. japonicus. The two technologies, combined with multivariate statistical analysis, can be promising methods to discriminate A. japonicus from different geographical areas. Copyright © 2016. Published by Elsevier Ltd.

Changes in collagenous tissue microstructures and distributions of cathepsin L in body wall of autolytic sea cucumber (Stichopus japonicus).

PubMed

Liu, Yu-Xin; Zhou, Da-Yong; Ma, Dong-Dong; Liu, Yan-Fei; Li, Dong-Mei; Dong, Xiu-Ping; Tan, Ming-Qian; Du, Ming; Zhu, Bei-Wei

2016-12-01

The autolysis of sea cucumber (Stichopus japonicus) was induced by ultraviolet (UV) irradiation, and the changes of microstructures of collagenous tissues and distributions of cathepsin L were investigated using histological and histochemical techniques. Intact collagen fibers in fresh S. japonicus dermis were disaggregated into collagen fibrils after UV stimuli. Cathepsin L was identified inside the surface of vacuoles in the fresh S. japonicus dermis cells. After the UV stimuli, the membranes of vacuoles and cells were fused together, and cathepsin L was released from cells and diffused into tissues. The density of cathepsin L was positively correlated with the speed and degree of autolysis in different layers of body wall. Our results revealed that lysosomal cathepsin L was released from cells in response to UV stimuli, which contacts and degrades the extracellular substrates such as collagen fibers, and thus participates in the autolysis of S. japonicus. Copyright © 2016 Elsevier Ltd. All rights reserved.

Parasitism of Argulus japonicus in cultured and wild fish of Guangdong, China with new record of three hosts.

PubMed

Alsarakibi, Muhamd; Wadeh, Hicham; Li, Guoqing

2014-02-01

This study aimed to demonstrate the ability of Argulus japonicus to infect a wide range of freshwater fishes, as well as to understand the effects of fish origin and host body size on the incidence of A. japonicus. Samples of cultured and wild fish were collected randomly from July 2010 to March 2013, using angling, long-lining, gill-netting, and trapping from rivers and fish farms in Guangdong province, South China. Eight fish species were found to be heavily infected including the common carp, the goldfish, the black carp, the silver carp, the brown trout, the rainbow trout, the mandarin fish, and the perch. Furthermore, the black carp, the brown trout, and the mandarin fish were recorded as new hosts for the first time. During the present study, a total of 2,271 fishes were examined, out of which 712 fishes were found to be infected by a total of 1,443 A. japonicus. Abundance and intensity of A. japonicus infection were significantly influenced by origin of fishes (cultured and wild) and total length (class I, <250 mm; class II, 250-350 mm; and class III, >350 mm) of fish species, whereas varied impacts on prevalence of infection were observed. The correlation between total length of fishes and prevalence of A. japonicus infection was variable, where no significant correlation was observed in the black carp, the silver carp, the mandarin fish, and the perch. In spite of the weak negative correlation between body size of the silver carp and prevalence of infection, A. japonicus was the most abundant and intensive in the silver carp. Thus, aquaculturists should pay particular attention to the control of these fish lice due to its host biodiversity.

49 CFR 1522.129 - Recordkeeping requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... VALIDATION FIRMS AND VALIDATORS TSA-Approved Validation Firms and Validators for the Certified Cargo Screening Program § 1522.129 Recordkeeping requirements. (a) Each validation firm must maintain records... operation as a validation firm, including the records listed below. (b) Each validation firm must retain the...

DNA Barcoding of genus Hexacentrus in China reveals cryptic diversity within Hexacentrus japonicus (Orthoptera, Tettigoniidae).

PubMed

Guo, Hui-Fang; Guan, Bei; Shi, Fu-Ming; Zhou, Zhi-Jun

2016-01-01

DNA barcoding has been proved successful to provide resolution beyond the boundaries of morphological information. Hence, a study was undertaken to establish DNA barcodes for all morphologically determined Hexacentrus species in China collections. In total, 83 specimens of five Hexacentrus species were barcoded using standard mitochondrial cytochrome c oxidase subunit I (COI) gene. Except for Hexacentrus japonicus, barcode gaps were present in the remaining Hexacentrus species. Taxon ID tree generated seven BOLD's barcode index numbers (BINs), four of which were in agreement with the morphological species. For Hexacentrus japonicus, the maximum intraspecific divergence (4.43%) produced a minimal overlap (0.64%), and 19 specimens were divided into three different BINs. There may be cryptic species within the current Hexacentrus japonicus. This study adds to a growing body of DNA barcodes that have become available for katydids, and shows that a DNA barcoding approach enables the identification of known Hexacentrus species with a very high resolution.

DNA Barcoding of genus Hexacentrus in China reveals cryptic diversity within Hexacentrus japonicus (Orthoptera, Tettigoniidae)

PubMed Central

Guo, Hui-Fang; Guan, Bei; Shi, Fu-Ming; Zhou, Zhi-Jun

2016-01-01

Abstract DNA barcoding has been proved successful to provide resolution beyond the boundaries of morphological information. Hence, a study was undertaken to establish DNA barcodes for all morphologically determined Hexacentrus species in China collections. In total, 83 specimens of five Hexacentrus species were barcoded using standard mitochondrial cytochrome c oxidase subunit I (COI) gene. Except for Hexacentrus japonicus, barcode gaps were present in the remaining Hexacentrus species. Taxon ID tree generated seven BOLD’s barcode index numbers (BINs), four of which were in agreement with the morphological species. For Hexacentrus japonicus, the maximum intraspecific divergence (4.43%) produced a minimal overlap (0.64%), and 19 specimens were divided into three different BINs. There may be cryptic species within the current Hexacentrus japonicus. This study adds to a growing body of DNA barcodes that have become available for katydids, and shows that a DNA barcoding approach enables the identification of known Hexacentrus species with a very high resolution. PMID:27408576

Comprehensive functional characterization of the glycoside hydrolase family 3 enzymes from Cellvibrio japonicus reveals unique metabolic roles in biomass saccharification.

PubMed

Nelson, Cassandra E; Attia, Mohamed A; Rogowski, Artur; Morland, Carl; Brumer, Harry; Gardner, Jeffrey G

2017-12-01

Lignocellulose degradation is central to the carbon cycle and renewable biotechnologies. The xyloglucan (XyG), β(1→3)/β(1→4) mixed-linkage glucan (MLG) and β(1→3) glucan components of lignocellulose represent significant carbohydrate energy sources for saprophytic microorganisms. The bacterium Cellvibrio japonicus has a robust capacity for plant polysaccharide degradation, due to a genome encoding a large contingent of Carbohydrate-Active enZymes (CAZymes), many of whose specific functions remain unknown. Using a comprehensive genetic and biochemical approach, we have delineated the physiological roles of the four C. japonicus glycoside hydrolase family 3 (GH3) members on diverse β-glucans. Despite high protein sequence similarity and partially overlapping activity profiles on disaccharides, these β-glucosidases are not functionally equivalent. Bgl3A has a major role in MLG and sophorose utilization, and supports β(1→3) glucan utilization, while Bgl3B underpins cellulose utilization and supports MLG utilization. Bgl3C drives β(1→3) glucan utilization. Finally, Bgl3D is the crucial β-glucosidase for XyG utilization. This study not only sheds the light on the metabolic machinery of C. japonicus, but also expands the repertoire of characterized CAZymes for future deployment in biotechnological applications. In particular, the precise functional analysis provided here serves as a reference for informed bioinformatics on the genomes of other Cellvibrio and related species. © 2017 Society for Applied Microbiology and John Wiley & Sons Ltd.

Gekko japonicus genome reveals evolution of adhesive toe pads and tail regeneration

PubMed Central

Liu, Yan; Zhou, Qian; Wang, Yongjun; Luo, Longhai; Yang, Jian; Yang, Linfeng; Liu, Mei; Li, Yingrui; Qian, Tianmei; Zheng, Yuan; Li, Meiyuan; Li, Jiang; Gu, Yun; Han, Zujing; Xu, Man; Wang, Yingjie; Zhu, Changlai; Yu, Bin; Yang, Yumin; Ding, Fei; Jiang, Jianping; Yang, Huanming; Gu, Xiaosong

2015-01-01

Reptiles are the most morphologically and physiologically diverse tetrapods, and have undergone 300 million years of adaptive evolution. Within the reptilian tetrapods, geckos possess several interesting features, including the ability to regenerate autotomized tails and to climb on smooth surfaces. Here we sequence the genome of Gekko japonicus (Schlegel's Japanese Gecko) and investigate genetic elements related to its physiology. We obtain a draft G. japonicus genome sequence of 2.55 Gb and annotated 22,487 genes. Comparative genomic analysis reveals specific gene family expansions or reductions that are associated with the formation of adhesive setae, nocturnal vision and tail regeneration, as well as the diversification of olfactory sensation. The obtained genomic data provide robust genetic evidence of adaptive evolution in reptiles. PMID:26598231

Influence of salinity on the early development and biochemical dynamics of a marine fish, Inimicus japonicus

NASA Astrophysics Data System (ADS)

Gong, Xu; Huang, Xuxiong; Wen, Wen

2018-03-01

Fertilised eggs of the devil stringer ( Inimicus japonicus) were incubated at different salinity levels (21, 25, 29, 33, and 37), and then the hatching performances, morphological parameters, and biochemical composition (protein, lipid and carbohydrate) of the larvae were assayed to determine the influence of salinity on the early development of I. japonicus. The tested salinity levels did not affect the times of hatching or mouth opening for yolk-sac larvae. However, the salinity significantly influenced the hatching and survival rates of open-mouthed larvae, as well as the morphology of yolk-sac larvae. The data indicated that 30.5 to 37.3 and 24.4 to 29.8 were suitable salinity ranges for the survival of embryos and larvae of I. japonicus, respectively. Larvae incubated at a salinity level of 29 had the greatest full lengths, and decreasing yolk volume was positively correlated with the environmental salinity. With increasing salinity, the individual dry weights of newly hatched larvae or open-mouthed larvae decreased significantly. Newly hatched larvae incubated at a salinity level of 29 had the greatest metabolic substrate contents and gross energy levels, while the openmouthed larvae's greatest values occurred at a salinity level of 25. Larvae incubated in the salinity range of 33 to 37 had the lowest nutritional reserves and energy values. Thus, the I. japonicus yolk-sac larvae acclimated more readily to the lower salinity level than the embryos, and higher salinity levels negatively influenced larval growth and development. In conclusion, the environmental salinity level should be maintained at 29-33 during embryogenesis and at 25-29 during early larval development for this species. Our results can be used to provide optimum aquaculture conditions for the early larval development of I. japonicus.

Influence of salinity on the early development and biochemical dynamics of a marine fish, Inimicus japonicus

NASA Astrophysics Data System (ADS)

Gong, Xu; Huang, Xuxiong; Wen, Wen

2017-05-01

Fertilised eggs of the devil stringer (Inimicus japonicus) were incubated at different salinity levels (21, 25, 29, 33, and 37), and then the hatching performances, morphological parameters, and biochemical composition (protein, lipid and carbohydrate) of the larvae were assayed to determine the influence of salinity on the early development of I. japonicus. The tested salinity levels did not affect the times of hatching or mouth opening for yolk-sac larvae. However, the salinity significantly influenced the hatching and survival rates of open-mouthed larvae, as well as the morphology of yolk-sac larvae. The data indicated that 30.5 to 37.3 and 24.4 to 29.8 were suitable salinity ranges for the survival of embryos and larvae of I. japonicus, respectively. Larvae incubated at a salinity level of 29 had the greatest full lengths, and decreasing yolk volume was positively correlated with the environmental salinity. With increasing salinity, the individual dry weights of newly hatched larvae or open-mouthed larvae decreased significantly. Newly hatched larvae incubated at a salinity level of 29 had the greatest metabolic substrate contents and gross energy levels, while the openmouthed larvae's greatest values occurred at a salinity level of 25. Larvae incubated in the salinity range of 33 to 37 had the lowest nutritional reserves and energy values. Thus, the I. japonicus yolk-sac larvae acclimated more readily to the lower salinity level than the embryos, and higher salinity levels negatively influenced larval growth and development. In conclusion, the environmental salinity level should be maintained at 29-33 during embryogenesis and at 25-29 during early larval development for this species. Our results can be used to provide optimum aquaculture conditions for the early larval development of I. japonicus.

32 CFR 701.129 - Exemptions for specific Marine Corps record systems.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 32 National Defense 5 2013-07-01 2013-07-01 false Exemptions for specific Marine Corps record systems. 701.129 Section 701.129 National Defense Department of Defense (Continued) DEPARTMENT OF THE NAVY... Exemptions for specific Marine Corps record systems. (a) [Reserved] (b) System identifier and name: (1...

32 CFR 701.129 - Exemptions for specific Marine Corps record systems.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 32 National Defense 5 2012-07-01 2012-07-01 false Exemptions for specific Marine Corps record systems. 701.129 Section 701.129 National Defense Department of Defense (Continued) DEPARTMENT OF THE NAVY... Exemptions for specific Marine Corps record systems. (a) [Reserved] (b) System identifier and name: (1...

7 CFR 800.129 - Certificating reinspection and review of weighing results.

Code of Federal Regulations, 2010 CFR

2010-01-01

... results. 800.129 Section 800.129 Agriculture Regulations of the Department of Agriculture (Continued... Certificating reinspection and review of weighing results. (a) General. Except as provided in paragraph (a)(1.... Except as provided in paragraph (b)(2) of this section, only the result of the reinspection service shall...

7 CFR 800.129 - Certificating reinspection and review of weighing results.

Code of Federal Regulations, 2011 CFR

2011-01-01

... results. 800.129 Section 800.129 Agriculture Regulations of the Department of Agriculture (Continued... Certificating reinspection and review of weighing results. (a) General. Except as provided in paragraph (a)(1.... Except as provided in paragraph (b)(2) of this section, only the result of the reinspection service shall...

46 CFR 129.375 - System grounding.

Code of Federal Regulations, 2014 CFR

2014-10-01

... Power Sources and Distribution Systems § 129.375 System grounding. (a) If a grounded distribution system is provided, there must be only one connection to ground, regardless of the number of power sources. This connection must be at the main switchboard. (b) On each metallic vessel, a grounded distribution...

46 CFR 129.375 - System grounding.

Code of Federal Regulations, 2013 CFR

2013-10-01

... Power Sources and Distribution Systems § 129.375 System grounding. (a) If a grounded distribution system is provided, there must be only one connection to ground, regardless of the number of power sources. This connection must be at the main switchboard. (b) On each metallic vessel, a grounded distribution...

46 CFR 129.375 - System grounding.

Code of Federal Regulations, 2012 CFR

2012-10-01

... Power Sources and Distribution Systems § 129.375 System grounding. (a) If a grounded distribution system is provided, there must be only one connection to ground, regardless of the number of power sources. This connection must be at the main switchboard. (b) On each metallic vessel, a grounded distribution...

46 CFR 129.440 - Emergency lighting.

Code of Federal Regulations, 2010 CFR

2010-10-01

... INSTALLATIONS Lighting Systems § 129.440 Emergency lighting. (a) A vessel of less than 100 gross tons must have... working (machinery) spaces below the main deck. (b) The emergency lighting required by paragraph (a) of... to an automatic battery-charger; and (4) Of enough capacity for 6 hours of continuous operation. ...

Involvement of three putative glucosyltransferases from the UGT72 family in flavonol glucoside/rhamnoside biosynthesis in Lotus japonicus seeds

PubMed Central

Yin, Qinggang; Shen, Guoan; Chang, Zhenzhan; Tang, Yuhong; Gao, Hongwen

2017-01-01

Abstract Flavonols are one of the largest groups of flavonoids that confer benefits for the health of plants and animals. Flavonol glycosides are the predominant flavonoids present in the model legume Lotus japonicus. The molecular mechanisms underlying the biosynthesis of flavonol glycosides as yet remain unknown in L. japonicus. In the present study, we identified a total of 188 UDP-glycosyltransferases (UGTs) in L. japonicus by genome-wide searching. Notably, 12 UGTs from the UGT72 family were distributed widely among L. japonicus chromosomes, expressed in all tissues, and showed different docking scores in an in silico bioinformatics docking analysis. Further enzymatic assays showed that five recombinant UGTs (UGT72AD1, UGT72AF1, UGT72AH1, UGT72V3, and UGT72Z2) exhibit activity toward flavonol, flavone, and isoflavone aglycones. In particular, UGT72AD1, UGT72AH1, and UGT72Z2 are flavonol-specific UGTs with different kinetic properties. In addition, the overexpression of UGT72AD1 and UGT72Z2 led to increased accumulation of flavonol rhamnosides in L. japonicus and Arabidopsis thaliana. Moreover, the increase of kaempferol 3-O-rhamnoside-7-O-rhamnoside in transgenic A. thaliana inhibited root growth as compared with the wild-type control. These results highlight the significance of the UGT72 family in flavonol glycosylation and the role of flavonol rhamnosides in plant growth. PMID:28204516

Cryptophane Nanoscale Assemblies Expand 129Xe NMR Biosensing.

PubMed

Zemerov, Serge D; Roose, Benjamin W; Greenberg, Mara L; Wang, Yanfei; Dmochowski, Ivan J

2018-06-19

Cryptophane-based biosensors are promising agents for the ultrasensitive detection of biomedically relevant targets via 129 Xe NMR. Dynamic light scattering revealed that cryptophanes form water-soluble aggregates tens to hundreds of nanometers in size. Acridine orange fluorescence quenching assays allowed quantitation of the aggregation state, with critical concentrations ranging from 200 nM to 600 nM, depending on the cryptophane species in solution. The addition of excess carbonic anhydrase (CA) protein target to a benzenesulfonamide-functionalized cryptophane biosensor (C8B) led to C8B disaggregation and produced the expected 1:1 C8B-CA complex. C8B showed higher affinity at 298 K for the cytoplasmic isozyme CAII than the extracellular CAXII isozyme, which is a biomarker of cancer. Using hyper-CEST NMR, we explored the role of stoichiometry in detecting these two isozymes. Under CA-saturating conditions, we observed that isozyme CAII produces a larger 129 Xe NMR chemical shift change (δ = 5.9 ppm, relative to free biosensor) than CAXII (δ = 2.7 ppm), which indicates the strong potential for isozyme-specific detection. However, stoichiometry-dependent chemical shift data indicated that biosensor disaggregation contributes to the observed 129 Xe NMR chemical shift change that is normally assigned to biosensor-target binding. Finally, we determined that monomeric cryptophane solutions improve hyper-CEST saturation contrast, which enables ultrasensitive detection of biosensor-protein complexes. These insights into cryptophane-solution behavior support further development of xenon biosensors, but will require reinterpretation of the data previously obtained for many water-soluble cryptophanes.

46 CFR 129.120 - Alternative standards.

Code of Federal Regulations, 2010 CFR

2010-10-01

... or less may meet the following requirements of the American Yacht and Boat Council Projects, where applicable, instead of § 129.340 of this part: (1) E-1, Bonding of Direct Current Systems. (2) E-8, AC Electrical Systems on Boats. (3) E-9, DC Electrical Systems on Boats. (b) An OSV with an electrical...

14 CFR 129.23 - Transport category cargo service airplanes: Increased zero fuel and landing weights.

Code of Federal Regulations, 2013 CFR

2013-01-01

...: Increased zero fuel and landing weights. 129.23 Section 129.23 Aeronautics and Space FEDERAL AVIATION... ENGAGED IN COMMON CARRIAGE General § 129.23 Transport category cargo service airplanes: Increased zero... (certificated under part 4b of the Civil Air Regulations effective before March 13, 1956) at increased zero fuel...

14 CFR 129.23 - Transport category cargo service airplanes: Increased zero fuel and landing weights.

Code of Federal Regulations, 2012 CFR

2012-01-01

...: Increased zero fuel and landing weights. 129.23 Section 129.23 Aeronautics and Space FEDERAL AVIATION... ENGAGED IN COMMON CARRIAGE General § 129.23 Transport category cargo service airplanes: Increased zero... (certificated under part 4b of the Civil Air Regulations effective before March 13, 1956) at increased zero fuel...

14 CFR 129.23 - Transport category cargo service airplanes: Increased zero fuel and landing weights.

Code of Federal Regulations, 2014 CFR

2014-01-01

...: Increased zero fuel and landing weights. 129.23 Section 129.23 Aeronautics and Space FEDERAL AVIATION... ENGAGED IN COMMON CARRIAGE General § 129.23 Transport category cargo service airplanes: Increased zero... (certificated under part 4b of the Civil Air Regulations effective before March 13, 1956) at increased zero fuel...

14 CFR 129.23 - Transport category cargo service airplanes: Increased zero fuel and landing weights.

Code of Federal Regulations, 2011 CFR

2011-01-01

...: Increased zero fuel and landing weights. 129.23 Section 129.23 Aeronautics and Space FEDERAL AVIATION... ENGAGED IN COMMON CARRIAGE General § 129.23 Transport category cargo service airplanes: Increased zero... (certificated under part 4b of the Civil Air Regulations effective before March 13, 1956) at increased zero fuel...

14 CFR 129.23 - Transport category cargo service airplanes: Increased zero fuel and landing weights.

Code of Federal Regulations, 2010 CFR

2010-01-01

...: Increased zero fuel and landing weights. 129.23 Section 129.23 Aeronautics and Space FEDERAL AVIATION... ENGAGED IN COMMON CARRIAGE General § 129.23 Transport category cargo service airplanes: Increased zero... (certificated under part 4b of the Civil Air Regulations effective before March 13, 1956) at increased zero fuel...

Effect of Bacillus baekryungensis YD13 supplemented in diets on growth performance and immune response of sea cucumber ( Apostichopus japonicus)

NASA Astrophysics Data System (ADS)

Yan, Fajun; Tian, Xiangli; Dong, Shuanglin

2014-10-01

The effect of a potential probiotic on the growth performance and immune response of sea cucumber ( Apostichopus japonicus) was investigated. Bacillus baekryungensis YD13 isolated from sea cucumber culturing ponds was added to sea cucumber basal feed as a probiotic in different doses (0, the control; 1×104 (YD134), 1×106 (YD136) and 1×108 (YD138) CFU g-1 of diet), and administered orally to A. japonicus (initial mean wet weight 5.44 g ± 0.17 g). The sea cucumbers were fed in 20 aquaria, 5 each treatment, for 60 d. At the end of growth trial, 20 sea cucumbers from each treatment were challenged with Vibrio splendidus. A. japonicus in YD134 and YD136 exhibited significantly better growth performance than control ( P < 0.05). Five non-specific immune parameters including lysozyme, acid phosphatase, alkaline phosphatase, superoxide dismutase and catalase in coelomic fluid were measured to evaluate the immune response of A. japonicus to the probiotics. Results showed that all parameters were significantly improved when YD11 was supplemented in the dose of 1×106 CFU g-1 ( P < 0.05). The cumulative incidence and mortality after the Vibrio splendidus challenge decreased significantly in sea cucumbers of YD136. Accordingly, 1×106 CFU g-1 of YD13 in diet was recommended for the growth promotion and immune enhancement of A. japonicus.

Metabolic rates and biochemical compositions of Apostichopus japonicus (Selenka) tissue during periods of inactivity

NASA Astrophysics Data System (ADS)

Bao, Jie; Dong, Shuanglin; Tian, Xiangli; Wang, Fang; Gao, Qinfeng; Dong, Yunwei

2010-03-01

Estivation, hibernation, and starvation are indispensable inactive states of sea cucumbers Apostichopus japonicus in nature and in culture ponds. Generally, temperature is the principal factor that induces estivation or hibernation in the sea cucumber. The present study provided insight into the physiological adaptations of A. japonicus during the three types of inactivity (hibernation, estivation, and starvation) by measuring the oxygen consumption rates ( Vo2) and biochemical compositions under laboratory conditions of low (3°C), normal (17°C) and high (24°C) temperature. The results show that the characteristics of A. japonicus in dormancy (hibernation and estivation) states were quite different from higher animals, such as fishes, amphibians, reptiles, and mammals, but more closely resembled a semi-dormant state. It was observed that the shift in the A. japonicus physiological state from normal to dormancy was a chronic rather than acute process, indicated by the gradual depression of metabolic rate. While metabolic rates declined 44.9% for the estivation group and 71.7% for the hibernation group, relative to initial rates, during the 36 d culture period, metabolic rates were not maintained at constant levels during these states. The metabolic depression processes for sea cucumbers in hibernation and estivation appeared to be a passive and an active metabolic suppression, respectively. In contrast, the metabolic rates (128.90±11.70 μg/g h) of estivating sea cucumbers were notably higher (107.85±6.31 μg/g h) than in starving sea cucumbers at 17°C, which indicated that the dormancy mechanism here, as a physiological inhibition, was not as efficient as in higher animals. Finally, the principle metabolic substrate or energy source of sea cucumbers in hibernation was lipid, whereas in estivation they mainly consumed protein in the early times and both protein and lipid thereafter.

49 CFR 37.129 - Types of service.

Code of Federal Regulations, 2010 CFR

2010-10-01

... DISABILITIES (ADA) Paratransit as a Complement to Fixed Route Service § 37.129 Types of service. (a) Except as provided in this section, complementary paratransit service for ADA paratransit eligible persons shall be origin-to-destination service. (b) Complementary paratransit service for ADA paratransit eligible persons...

77 FR 27021 - Foreign-Trade Zone 129-Bellingham, WA; Application for Reorganization Under Alternative Site...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-08

... FTZ 131 zone projects under FTZ 129. FTZ 130 was approved by the Board on September 4, 1986 (51 FR... DEPARTMENT OF COMMERCE Foreign-Trade Zones Board [(B-32-2012)] Foreign-Trade Zone 129--Bellingham... to the Foreign-Trade Zones (FTZ) Board (the Board) by the Port of Bellingham, grantee of FTZ 129...

(-)-Epicatechin derivate from Orostachys japonicus as potential inhibitor of the human butyrylcholinesterase.

PubMed

Kim, Jang Hoon; Lee, Sang-Hyun; Lee, Hyun Woo; Sun, Ya Nan; Jang, Won-Hee; Yang, Seo-Young; Jang, Hae-Dong; Kim, Young Ho

2016-10-01

Cholinesterase inhibitors block the bioconversion of neurotransmitters by cholinesterase in the nervous system. Epicatechin derivatives (1, 3 and 5), polyphenols (6 and 7) from Orostachys japonicus, and catechin derivatives (2 and 4) from our in-house library were evaluated for their inhibitory activity on cholinesterase. Compound 5 exhibited IC50 values of 58.3±2.4 and 17.8±3.8μg/mL on AChE and BuChE, respectively. Compound 5 inhibited BuChE more strongly than AChE through a competitive behavior. In silico binding positions of 5 in the active site were predicted using Autodock 4.2 and processed in a 10000-ps molecular dynamics simulation to assess the stability of compound 5 binding. Copyright © 2016 Elsevier B.V. All rights reserved.

The Shock and Vibration Digest. Volume 17. Number 6

DTIC Science & Technology

1985-06-01

Australia 3032 Friction Intl. J. Impact Engrg., Z (2), pp 151-167 E. Marui , S. Kato (1984), 14 figs, I table, 17 refs Gifu Univ., 1-1, Yanagido, Gifu-shi 501...Martinez-Sanchez............... 1153 * Jensen, J.J ................. 1094 Marui , F ............... 1202 Jery, B........................ 1073 Marulo,F

Characterization and expression analysis of a complement component gene in sea cucumber ( Apostichopus japonicus)

NASA Astrophysics Data System (ADS)

Chen, Zhong; Zhou, Zunchun; Yang, Aifu; Dong, Ying; Guan, Xiaoyan; Jiang, Bei; Wang, Bai

2015-12-01

The complement system plays a crucial role in the innate immune system of animals. It can be activated by distinct yet overlapping classical, alternative and lectin pathways. In the alternative pathway, complement factor B (Bf) serves as the catalytic subunit of complement component 3 (C3) convertase, which plays the central role among three activation pathways. In this study, the Bf gene in sea cucumber ( Apostichopus japonicus), termed AjBf, was obtained by rapid amplification of cDNA ends (RACE). The full-length cDNA of AjBf was 3231 bp in length barring the poly (A) tail. It contained an open reading frame (ORF) of 2742 bp encoding 913 amino acids, a 105 bp 5'-UTR (5'-terminal untranslated region) and a 384 bp 3'-UTR. AjBf was a mosaic protein with six CCP (complement control protein) domains, a VWA (von Willebrand factor A) domain, and a serine protease domain. The deduced molecular weight of AjBf protein was 101 kDa. Quantitative real time PCR (qRT-PCR) analysis indicated that the expression level of AjBf in A. japonicus was obviously higher at larval stage than that at embryonic stage. Expression detection in different tissues showed that AjBf expressed higher in coelomocytes than in other four tissues. In addation, AjBf expression in different tissues was induced significantly after LPS or PolyI:C challenge. These results indicated that AjBf plays an important role in immune responses to pathogen infection.

Reproductive biology of Nemipterus japonicus (Bloch, 1791) from the coastal waters of Bintulu (South China Sea), Sarawak, Malaysia.

PubMed

Nettely, T; Rajaee, A H; Denil, N A; Idris, M H; Nesarul, M H; Amin, S M Nurul; Hena, M K Abu

2016-07-01

Samples of threadfin breams Nemipterus japonicus were collected from a village in Kuala Nyalau and a fish landing centre at Bintulu from April 2013 to March 2014. A total of 360 individuals of N. japonicus (214 male and 146 female) were used in this reproductive study.? The total length (TL) of individuals were measured to the nearest 0.1 cm and body weight (BW) was recorded to the nearest 0.1 g. Month-wise distribution of the sexes was significantly higher for males in September and March, while in the month of May the number of females was significantly higher (X(2) = 6.53; P < 0.05). Males showed a preponderance in the size-class of 19.0-20.9 cm (X(2) = 80.24; P < 0.001), 21.0 -22.9 cm (X(2) = 56.39; P < 0.001) and 23.0 -24.9 cm (X(2) = 17; P < 0.001). The gonadosomatic index (GSI) values of N. japonicus ranged from 0.07 to 0.19 for males and 0.34 to 4.99 for females. Females had higher GSI than males throughout the study period. For males, a higher GSI (0.11-0.19) was observed during January to February, while it was found to be higher (2.73-4.99) for females during January to March, indicating the spawning season. The present study revealed that ovarian maturity based on histological analysis of N. japonicus was classified into seven stages namely, immature (I), immature (II), maturing (III), mature (IV), ripe (V), spawning (VI) and spent (VII). The fecundity of N. japonicus was estimated to be within a range of 19221 to 85923 with higher GSI (3.08-6.78) from the coastal waters of Bintulu, Sarawak.

RNA sequencing analysis to capture the transcriptome landscape during skin ulceration syndrome progression in sea cucumber Apostichopus japonicus.

PubMed

Yang, Aifu; Zhou, Zunchun; Pan, Yongjia; Jiang, Jingwei; Dong, Ying; Guan, Xiaoyan; Sun, Hongjuan; Gao, Shan; Chen, Zhong

2016-06-14

Sea cucumber Apostichopus japonicus is an important economic species in China, which is affected by various diseases; skin ulceration syndrome (SUS) is the most serious. In this study, we characterized the transcriptomes in A. japonicus challenged with Vibrio splendidus to elucidate the changes in gene expression throughout the three stages of SUS progression. RNA sequencing of 21 cDNA libraries from various tissues and developmental stages of SUS-affected A. japonicus yielded 553 million raw reads, of which 542 million high-quality reads were generated by deep-sequencing using the Illumina HiSeq™ 2000 platform. The reference transcriptome comprised a combination of the Illumina reads, 454 sequencing data and Sanger sequences obtained from the public database to generate 93,163 unigenes (average length, 1,052 bp; N50 = 1,575 bp); 33,860 were annotated. Transcriptome comparisons between healthy and SUS-affected A. japonicus revealed greater differences in gene expression profiles in the body walls (BW) than in the intestines (Int), respiratory trees (RT) and coelomocytes (C). Clustering of expression models revealed stable up-regulation as the main pattern occurring in the BW throughout the three stages of SUS progression. Significantly affected pathways were associated with signal transduction, immune system, cellular processes, development and metabolism. Ninety-two differentially expressed genes (DEGs) were divided into four functional categories: attachment/pathogen recognition (17), inflammatory reactions (38), oxidative stress response (7) and apoptosis (30). Using quantitative real-time PCR, twenty representative DEGs were selected to validate the sequencing results. The Pearson's correlation coefficient (R) of the 20 DEGs ranged from 0.811 to 0.999, which confirmed the consistency and accuracy between these two approaches. Dynamic changes in global gene expression occur during SUS progression in A. japonicus. Elucidation of these changes is important

Differential gene expression in the intestine of sea cucumber (Apostichopus japonicus) under low and high salinity conditions.

PubMed

Zhang, Libin; Feng, Qiming; Sun, Lina; Ding, Kui; Huo, Da; Fang, Yan; Zhang, Tao; Yang, Hongsheng

2018-03-01

Sea cucumber, Apostichopus japonicus is an important species for aquaculture, and its behavior and physiology can change in response to changing salinity conditions. For this reason, it is important to understand the molecular responses of A. japonicus when exposed to ambient changes in salinity. In this study, RNA-Seq provided a general overview of the gene expression profiles in the intestine of A. japonicus exposed to high salinity (SD40), normal salinity (SD30) and low salinity (SD20) environments. Screening for differentially expressed genes (DEGs) using the NOISeq method identified 109, 100, and 89 DEGs based on a fold change of ≥2 and divergence probability ≥0.8 according to the comparisons of SD20 vs. SD30, SD20 vs.SD40, and SD30 vs. SD40, respectively. Gene ontology analysis showed that the terms "metabolic process" and "catalytic activity" comprised the most enriched DEGs. These fell into the categories of "biological process" and "molecular function". While "cell" and "cell part" had the most enriched DEGs in the category of "cellular component". With these DEGs mapping to 2119, 159, and 160 pathways in the Kyoto Encyclopedia of Genes and Genomes database. Of these 51, 2, and 57 pathways were significantly enriched, respectively. The osmosis-specific DEGs identified in this study of A. japonicus will be important targets for further studies to understand the biochemical mechanisms involved with the adaption of sea cucumbers to changes in salinity. Copyright © 2017. Published by Elsevier Inc.

Lotus japonicus flowers are defended by a cyanogenic β-glucosidase with highly restricted expression to essential reproductive organs.

PubMed

Lai, Daniela; Pičmanová, Martina; Abou Hachem, Maher; Motawia, Mohammed Saddik; Olsen, Carl Erik; Møller, Birger Lindberg; Rook, Fred; Takos, Adam M

2015-09-01

Flowers and leaves of Lotus japonicus contain α-, β-, and γ-hydroxynitrile glucoside (HNG) defense compounds, which are bioactivated by β-glucosidase enzymes (BGDs). The α-HNGs are referred to as cyanogenic glucosides because their hydrolysis upon tissue disruption leads to release of toxic hydrogen cyanide gas, which can deter herbivore feeding. BGD2 and BGD4 are HNG metabolizing BGD enzymes expressed in leaves. Only BGD2 is able to hydrolyse the α-HNGs. Loss of function mutants of BGD2 are acyanogenic in leaves but fully retain cyanogenesis in flowers pointing to the existence of an alternative cyanogenic BGD in flowers. This enzyme, named BGD3, is identified and characterized in this study. Whereas all floral tissues contain α-HNGs, only those tissues in which BGD3 is expressed, the keel and the enclosed reproductive organs, are cyanogenic. Biochemical analysis, active site architecture molecular modelling, and the observation that L. japonicus accessions lacking cyanogenic flowers contain a non-functional BGD3 gene, all support the key role of BGD3 in floral cyanogenesis. The nectar of L. japonicus flowers was also found to contain HNGs and additionally their diglycosides. The observed specialisation in HNG based defence in L. japonicus flowers is discussed in the context of balancing the attraction of pollinators with the protection of reproductive structures against herbivores.

46 CFR 129.340 - Cable and wiring.

Code of Federal Regulations, 2011 CFR

2011-10-01

... buildup of condensation. (b) Each cable and wire must— (1) Have stranded copper conductors with sufficient... Power Sources and Distribution Systems § 129.340 Cable and wiring. (a) If individual wires, rather than cables, are used in systems operating at a potential of greater than 50 volts, the wire and associated...

46 CFR 129.340 - Cable and wiring.

Code of Federal Regulations, 2013 CFR

2013-10-01

... buildup of condensation. (b) Each cable and wire must— (1) Have stranded copper conductors with sufficient... Power Sources and Distribution Systems § 129.340 Cable and wiring. (a) If individual wires, rather than cables, are used in systems operating at a potential of greater than 50 volts, the wire and associated...

46 CFR 129.340 - Cable and wiring.

Code of Federal Regulations, 2014 CFR

2014-10-01

... buildup of condensation. (b) Each cable and wire must— (1) Have stranded copper conductors with sufficient... Power Sources and Distribution Systems § 129.340 Cable and wiring. (a) If individual wires, rather than cables, are used in systems operating at a potential of greater than 50 volts, the wire and associated...

46 CFR 129.340 - Cable and wiring.

Code of Federal Regulations, 2012 CFR

2012-10-01

... buildup of condensation. (b) Each cable and wire must— (1) Have stranded copper conductors with sufficient... Power Sources and Distribution Systems § 129.340 Cable and wiring. (a) If individual wires, rather than cables, are used in systems operating at a potential of greater than 50 volts, the wire and associated...

Schizosaccharomyces japonicus: the fission yeast is a fusion of yeast and hyphae.

PubMed

Niki, Hironori

2014-03-01

The clade of Schizosaccharomyces includes 4 species: S. pombe, S. octosporus, S. cryophilus, and S. japonicus. Although all 4 species exhibit unicellular growth with a binary fission mode of cell division, S. japonicus alone is dimorphic yeast, which can transit from unicellular yeast to long filamentous hyphae. Recently it was found that the hyphal cells response to light and then synchronously activate cytokinesis of hyphae. In addition to hyphal growth, S. japonicas has many properties that aren't shared with other fission yeast. Mitosis of S. japonicas is referred to as semi-open mitosis because dynamics of nuclear membrane is an intermediate mode between open mitosis and closed mitosis. Novel genetic tools and the whole genomic sequencing of S. japonicas now provide us with an opportunity for revealing unique characters of the dimorphic yeast. © 2013 The Author. Yeast Published by John Wiley & Sons Ltd.

Ontogeny of antipredator performance in hatchery-reared Japanese anchovy Engraulis japonicus larvae exposed to visual or tactile predators in relation to turbidity.

PubMed

Ohata, R; Masuda, R; Yamashita, Y

2011-12-01

Laboratory experiments revealed distinct effects of turbidity on the survival of Japanese anchovy Engraulis japonicus larvae when exposed to either visual (jack mackerel Trachurus japonicus) or tactile (moon jellyfish Aurelia aurita) predators. The experiments were conducted in 30 l tanks with three levels of turbidity obtained by dissolving 0, 50 or 300 mg l(-1) of kaolin. Predators were introduced to experimental tanks followed by larvae of E. japonicus ranging from 5 to 25 mm standard lengths (L(s) ). When exposed to T. japonicus, the mean survival rate of larvae was significantly higher in 300 mg l(-1) treatments compared to the other turbidity levels. When exposed to A. aurita, however, there was no difference in the survival rates among different turbidity treatments. The survival rates when exposed to either predator improved with larval growth. The logistic survivorship models for E. japonicus larvae when exposed to A. aurita had an inflection point at c. 12 mm L(s) , suggesting that their size refuge from A. aurita is close to this value. Comparison to a previous study suggests a high vulnerability of shirasu (long and transparent) fish larvae to jellyfish predation under turbidity. This study indicates that anthropogenic increases of turbidity in coastal waters may increase the relative effect of jellyfish predation on fish larvae. © 2011 The Authors. Journal of Fish Biology © 2011 The Fisheries Society of the British Isles.

RNA-seq Transcriptome Analysis of Panax japonicus, and Its Comparison with Other Panax Species to Identify Potential Genes Involved in the Saponins Biosynthesis

PubMed Central

Rai, Amit; Yamazaki, Mami; Takahashi, Hiroki; Nakamura, Michimi; Kojoma, Mareshige; Suzuki, Hideyuki; Saito, Kazuki

2016-01-01

The Panax genus has been a source of natural medicine, benefitting human health over the ages, among which the Panax japonicus represents an important species. Our understanding of several key pathways and enzymes involved in the biosynthesis of ginsenosides, a pharmacologically active class of metabolites and a major chemical constituents of the rhizome extracts from the Panax species, are limited. Limited genomic information, and lack of studies on comparative transcriptomics across the Panax species have restricted our understanding of the biosynthetic mechanisms of these and many other important classes of phytochemicals. Herein, we describe Illumina based RNA sequencing analysis to characterize the transcriptome and expression profiles of genes expressed in the five tissues of P. japonicus, and its comparison with other Panax species. RNA sequencing and de novo transcriptome assembly for P. japonicus resulted in a total of 135,235 unigenes with 78,794 (58.24%) unigenes being annotated using NCBI-nr database. Transcriptome profiling, and gene ontology enrichment analysis for five tissues of P. japonicus showed that although overall processes were evenly conserved across all tissues. However, each tissue was characterized by several unique unigenes with the leaves showing the most unique unigenes among the tissues studied. A comparative analysis of the P. japonicus transcriptome assembly with publically available transcripts from other Panax species, namely, P. ginseng, P. notoginseng, and P. quinquefolius also displayed high sequence similarity across all Panax species, with P. japonicus showing highest similarity with P. ginseng. Annotation of P. japonicus transcriptome resulted in the identification of putative genes encoding all enzymes from the triterpene backbone biosynthetic pathways, and identified 24 and 48 unigenes annotated as cytochrome P450 (CYP) and glycosyltransferases (GT), respectively. These CYPs and GTs annotated unigenes were conserved across

Transcriptome Response Mediated by Cold Stress in Lotus japonicus.

PubMed

Calzadilla, Pablo I; Maiale, Santiago J; Ruiz, Oscar A; Escaray, Francisco J

2016-01-01

Members of the Lotus genus are important as agricultural forage sources under marginal environmental conditions given their high nutritional value and tolerance of various abiotic stresses. However, their dry matter production is drastically reduced in cooler seasons, while their response to such conditions is not well studied. This paper analyzes cold acclimation of the genus by studying Lotus japonicus over a stress period of 24 h. High-throughput RNA sequencing was used to identify and classify 1077 differentially expressed genes, of which 713 were up-regulated and 364 were down-regulated. Up-regulated genes were principally related to lipid, cell wall, phenylpropanoid, sugar, and proline regulation, while down-regulated genes affected the photosynthetic process and chloroplast development. Together, a total of 41 cold-inducible transcription factors were identified, including members of the AP2/ERF, NAC, MYB, and WRKY families; two of them were described as putative novel transcription factors. Finally, DREB1/CBFs were described with respect to their cold stress expression profiles. This is the first transcriptome profiling of the model legume L. japonicus under cold stress. Data obtained may be useful in identifying candidate genes for breeding modified species of forage legumes that more readily acclimate to low temperatures.

Construction of a Lotus japonicus late nodulin expressed sequence tag library and identification of novel nodule-specific genes.

PubMed Central

Szczyglowski, K; Hamburger, D; Kapranov, P; de Bruijn, F J

1997-01-01

A range of novel expressed sequence tags (ESTs) associated with late developmental events during nodule organogenesis in the legume Lotus japonicus were identified using mRNA differential display; 110 differentially displayed polymerase chain reaction products were cloned and analyzed. Of 88 unique cDNAs obtained, 22 shared significant homology to DNA/protein sequences in the respective databases. This group comprises, among others, a nodule-specific homolog of protein phosphatase 2C, a peptide transporter protein, and a nodule-specific form of cytochrome P450. RNA gel-blot analysis of 16 differentially displayed ESTs confirmed their nodule-specific expression pattern. The kinetics of mRNA accumulation of the majority of the ESTs analyzed were found to resemble the expression pattern observed for the L. japonicus leghemoglobin gene. These results indicate that the newly isolated molecular markers correspond to genes induced during late developmental stages of L. japonicus nodule organogenesis and provide important, novel tools for the study of nodulation. PMID:9276951

49 CFR 571.129 - Standard No. 129; New non-pneumatic tires for passenger cars.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 6 2010-10-01 2010-10-01 false Standard No. 129; New non-pneumatic tires for... VEHICLE SAFETY STANDARDS Federal Motor Vehicle Safety Standards § 571.129 Standard No. 129; New non... rating; and specifies labeling requirements for non-pneumatic spare tires. S2Application. This standard...

49 CFR 571.129 - Standard No. 129; New non-pneumatic tires for passenger cars.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 6 2011-10-01 2011-10-01 false Standard No. 129; New non-pneumatic tires for... VEHICLE SAFETY STANDARDS Federal Motor Vehicle Safety Standards § 571.129 Standard No. 129; New non... rating; and specifies labeling requirements for non-pneumatic spare tires. S2Application. This standard...

49 CFR 571.129 - Standard No. 129; New non-pneumatic tires for passenger cars.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 49 Transportation 6 2014-10-01 2014-10-01 false Standard No. 129; New non-pneumatic tires for... VEHICLE SAFETY STANDARDS Federal Motor Vehicle Safety Standards § 571.129 Standard No. 129; New non... rating; and specifies labeling requirements for non-pneumatic spare tires. S2Application. This standard...

49 CFR 571.129 - Standard No. 129; New non-pneumatic tires for passenger cars.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 49 Transportation 6 2012-10-01 2012-10-01 false Standard No. 129; New non-pneumatic tires for... VEHICLE SAFETY STANDARDS Federal Motor Vehicle Safety Standards § 571.129 Standard No. 129; New non... rating; and specifies labeling requirements for non-pneumatic spare tires. S2Application. This standard...

The application of compound-specific isotope analysis of fatty acids for traceability of sea cucumber (Apostichopus japonicus) in the coastal areas of China.

PubMed

Liu, Yu; Zhang, Xufeng; Li, Ying; Wang, Haixia

2017-11-01

Geographical origin traceability is an important issue for controlling the quality of seafood and safeguarding the interest of consumers. In the present study, a new method of compound-specific isotope analysis (CSIA) of fatty acids was established to evaluate its applicability in establishing the origin traceability of Apostichopus japonicus in the coastal areas of China. Moreover, principal component analysis (PCA) and discriminant analysis (DA) were applied to distinguish between the origins of A. japonicus. The results show that the stable carbon isotope compositions of fatty acids of A. japonicus significantly differ in terms of both season and origin. They also indicate that the stable carbon isotope composition of fatty acids could effectively discriminate between the origins of A. japonicus, except for between Changhai Island and Zhangzi Island in the spring of 2016 because of geographical proximity or the similarity of food sources. The fatty acids that have the highest contribution to identifying the geographical origins of A. japonicus are C22:6n-3, C16:1n-7, C20:5n-3, C18:0 and C23:1n-9, when considering the fatty acid contents, the stable carbon isotope composition of fatty acids and the results of the PCA and DA. We conclude that CSIA of fatty acids, combined with multivariate statistical analysis such as PCA and DA, may be an effective tool for establishing the traceability of A. japonicus in the coastal areas of China. The relevant conclusions of the present study provide a new method for determining the traceability of seafood or other food products. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

Crude oil exposure results in oxidative stress-mediated dysfunctional development and reproduction in the copepod Tigriopus japonicus and modulates expression of cytochrome P450 (CYP) genes.

PubMed

Han, Jeonghoon; Won, Eun-Ji; Hwang, Dae-Sik; Shin, Kyung-Hoon; Lee, Yong Sung; Leung, Kenneth Mei-Yee; Lee, Su-Jae; Lee, Jae-Seong

2014-07-01

In this study, we investigated the effects of the water-accommodated fraction (WAF) of crude oil on the development and reproduction of the intertidal copepod Tigriopus japonicus through life-cycle experiments. Furthermore, we investigated the mechanisms underlying the toxic effects of WAF on this benthic organism by studying expression patterns of cytochrome P450 (CYP) genes. Development of T. japonicus was delayed and molting was interrupted in response to WAF exposure. Hatching rate was also significantly reduced in response to WAF exposure. Activities of antioxidant enzymes such as glutathione S-transferase (GST), glutathione reductase (GR), and catalase (CAT) were increased by WAF exposure in a concentration-dependent manner. These results indicated that WAF exposure resulted in oxidative stress, which in turn was associated with dysfunctional development and reproduction. To evaluate the involvement of cytochrome P450 (CYP) genes, we cloned the entire repertoire of CYP genes in T. japonicus (n=52) and found that the CYP genes belonged to five different clans (i.e., Clans 2, 3, 4, mitochondrial, and 20). We then examined expression patterns of these 52 CYP genes in response to WAF exposure. Three TJ-CYP genes (CYP3024A2, CYP3024A3, and CYP3027C2) belonging to CYP clan 3 were significantly induced by WAF exposure in a time- and concentration-dependent manner. We identified aryl hydrocarbon responsive elements (AhRE), xenobiotic responsive elements (XREs), and metal response elements (MRE) in the promoter regions of these three CYP genes, suggesting that these genes are involved in detoxification of toxicants. Overall, our results indicate that WAF can trigger oxidative stress and thus induce dysfunctional development and reproduction in the copepod T. japonicus. Furthermore, we identified three TJ-CYP genes that represent potential biomarkers of oil pollution. Copyright © 2014 Elsevier B.V. All rights reserved.

Interspecific larval competition between invasive Aedes japonicus and native Aedes triseriatus (Diptera: Culicidae) and adult longevity.

PubMed

Alto, Barry W

2011-03-01

The Asian rock pool mosquito Aedes japonicus (Theobald) inhabits natural and artificial container habitats, some of which are occupied by the native treehole mosquitoAedes triseriatus (Say), a vector of LaCrosse encephalitis virus. A laboratory experiment was used to evaluate the effects of nutrient limitation and interspecific interactions between these species. The goal was to address two related hypotheses. First, interspecific interactions between these species show competitive asymmetry with the invasive mosquito Ae. japonicus being favored over Ae. triseriatus. Second, competitive stress at the larval stage alters adult longevity. There was minimal evidence for competitive asymmetry between these two species. Mosquito and population performance showed clear negative density-dependent effects with similar effects of intra- and interspecific interactions. Only Ae. japonicus development time showed competitive asymmetry over Ae. triseriatus, providing weak support for the first hypothesis. For both species, competition resulted in lower adult longevity compared with low competition, providing support for the second hypothesis. These results suggest both species are similarly affected by intra- and interspecific competition and underscore the importance of the effects of larval competition that continue into adulthood and alter parameters important to transmission of vector-borne diseases.

Effects of stomatal development on stomatal conductance and on stomatal limitation of photosynthesis in Syringa oblata and Euonymus japonicus Thunb.

PubMed

Wu, Bing-Jie; Chow, Wah Soon; Liu, Yu-Jun; Shi, Lei; Jiang, Chuang-Dao

2014-12-01

During leaf development, the increase in stomatal conductance cannot meet photosynthetic demand for CO2, thus leading to stomatal limitation of photosynthesis (Ls). Considering the crucial influences of stomatal development on stomatal conductance, we speculated whether stomatal development limits photosynthesis to some extent. To test this hypothesis, stomatal development, stomatal conductance and photosynthesis were carefully studied in both Syringa oblata (normal greening species) and Euonymus japonicus Thunb (delayed greening species). Our results show that the size of stomata increased gradually with leaf expansion, resulting in increased stomatal conductance up to the time of full leaf expansion. During this process, photosynthesis also increased steadily. Compared to that in S. oblata, the development of chloroplasts in E. japonicus Thunb was obviously delayed, leading to a delay in the improvement of photosynthetic capacity. Further analysis revealed that before full leaf expansion, stomatal limitation increased rapidly in both S. oblata and E. japonicus Thunb; after full leaf expansion, stomatal limitation continually increased in E. japonicus Thunb. Accordingly, we suggested that the enhancement of photosynthetic capacity is the main factor leading to stomatal limitation during leaf development but that stomatal development can alleviate stomatal limitation with the increase of photosynthesis by controlling gas exchange. Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

7 CFR 1703.129 - Appeals.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 11 2010-01-01 2010-01-01 false Appeals. 1703.129 Section 1703.129 Agriculture Regulations of the Department of Agriculture (Continued) RURAL UTILITIES SERVICE, DEPARTMENT OF AGRICULTURE RURAL DEVELOPMENT Distance Learning and Telemedicine Grant Program § 1703.129 Appeals. All qualifying...

7 CFR 1703.129 - Appeals.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 7 Agriculture 11 2011-01-01 2011-01-01 false Appeals. 1703.129 Section 1703.129 Agriculture Regulations of the Department of Agriculture (Continued) RURAL UTILITIES SERVICE, DEPARTMENT OF AGRICULTURE RURAL DEVELOPMENT Distance Learning and Telemedicine Grant Program § 1703.129 Appeals. All qualifying...

7 CFR 1703.129 - Appeals.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 7 Agriculture 11 2013-01-01 2013-01-01 false Appeals. 1703.129 Section 1703.129 Agriculture Regulations of the Department of Agriculture (Continued) RURAL UTILITIES SERVICE, DEPARTMENT OF AGRICULTURE RURAL DEVELOPMENT Distance Learning and Telemedicine Grant Program § 1703.129 Appeals. All qualifying...

7 CFR 1703.129 - Appeals.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 7 Agriculture 11 2014-01-01 2014-01-01 false Appeals. 1703.129 Section 1703.129 Agriculture Regulations of the Department of Agriculture (Continued) RURAL UTILITIES SERVICE, DEPARTMENT OF AGRICULTURE RURAL DEVELOPMENT Distance Learning and Telemedicine Grant Program § 1703.129 Appeals. All qualifying...

7 CFR 1703.129 - Appeals.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 7 Agriculture 11 2012-01-01 2012-01-01 false Appeals. 1703.129 Section 1703.129 Agriculture Regulations of the Department of Agriculture (Continued) RURAL UTILITIES SERVICE, DEPARTMENT OF AGRICULTURE RURAL DEVELOPMENT Distance Learning and Telemedicine Grant Program § 1703.129 Appeals. All qualifying...

7 CFR 60.129 - USDA.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 7 Agriculture 3 2014-01-01 2014-01-01 false USDA. 60.129 Section 60.129 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections... AND SHELLFISH General Provisions Definitions § 60.129 USDA. USDA means the United States Department of...

7 CFR 60.129 - USDA.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 7 Agriculture 3 2011-01-01 2011-01-01 false USDA. 60.129 Section 60.129 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections... AND SHELLFISH General Provisions Definitions § 60.129 USDA. USDA means the United States Department of...

7 CFR 60.129 - USDA.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 7 Agriculture 3 2012-01-01 2012-01-01 false USDA. 60.129 Section 60.129 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections... AND SHELLFISH General Provisions Definitions § 60.129 USDA. USDA means the United States Department of...

7 CFR 60.129 - USDA.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 7 Agriculture 3 2013-01-01 2013-01-01 false USDA. 60.129 Section 60.129 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections... AND SHELLFISH General Provisions Definitions § 60.129 USDA. USDA means the United States Department of...

Effect of food on specific dynamic action (SDA) of green and red types of sea cucumber ( Apostichopus japonicus Selenka)

NASA Astrophysics Data System (ADS)

Bao, Jie; Jiang, Hongbo; Dong, Shuanglin; Tian, Xiangli

2017-10-01

Specific dynamic action (SDA), the energy expended on all physiological processes that is associated with meal digestion and absorption, is strongly affected by food type. Effects of formulated diet (FMD), macroalgae (ALG) and sea mud (SMD) diets on the postprandial metabolic response of the green type and the red type of sea cucumber ( Apostichopus japonicus) were studied in order to understand their feeding physiology. Food offered to A. japonicus was different in protein, lipid content and energy but not in meal mass. SDA of A. japonicus resulted in a 1.3-2.7 folds of increase in oxygen consumption that can persist for up to 4.8-31.7 h after digesting three different diets. In a given type of sea cucumber, the magnitude of SDA was the highest when fed with FMD, medium with ALG, and the lowest with SMD, which is probably due to the differences in diet components and protein contents. The red type sea cucumber showed greater SDA magnitude than the green type with each diet treatment, which might result from the difference in factorial scope between the two types of sea cucumber. However, the smallest magnitude or even no difference was observed between the two types of A. japonicus in SMD group, perhaps owing to the poor nutrition and digestion of sea mud.

Suppression of murine preadipocyte differentiation and reduction of visceral fat accumulation by a Petasites japonicus ethanol extract in mice fed a high-fat diet.

PubMed

Watanabe, Takayuki; Hata, Keishi; Hiwatashi, Kazuyuki; Hori, Kazuyuki; Suzuki, Nao; Itoh, Hideaki

2010-01-01

We investigated in this study the anti-obesity effect of an extract of Petasites japonicus (a culinary vegetable from Eastern Asia) on a murine adipocyte cell line (3T3-L1) and on diet-induced obesity-prone mice. An ethanol extract of P. japonicus. (PJET) suppressed 3T3-L1 preadipocyte differentiation; however, a hot water extract of P. japonicus (PJHW) exhibited no effect on cell differentiation. PJET significantly attenuated three adipogenetic transcription factors, peroxisome proliferator-activated receptor gamma2, CCAAT/enhancer-binding protein and sterol regulatory element-binding protein 1C, at the mRNA level and suppressed the gene expression of fatty acid synthetase. An experiment with diet-induced obesity-prone C57BL/6J mice showed that PJET lowered the body weight gain and visceral fat tissue accumulation, and ameliorated the plasma cholesterol concentration. These findings suggest that P. japonicus might be an effective food against obesity.

31 CFR 129.5 - Confidentiality.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 31 Money and Finance: Treasury 1 2013-07-01 2013-07-01 false Confidentiality. 129.5 Section 129.5 Money and Finance: Treasury Regulations Relating to Money and Finance MONETARY OFFICES, DEPARTMENT OF THE TREASURY PORTFOLIO INVESTMENT SURVEY REPORTING § 129.5 Confidentiality. (a) Information collected...

31 CFR 129.5 - Confidentiality.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 31 Money and Finance: Treasury 1 2014-07-01 2014-07-01 false Confidentiality. 129.5 Section 129.5 Money and Finance: Treasury Regulations Relating to Money and Finance MONETARY OFFICES, DEPARTMENT OF THE TREASURY PORTFOLIO INVESTMENT SURVEY REPORTING § 129.5 Confidentiality. (a) Information collected...

Molecular and morphologic approaches to discrimination of variability patterns in chub mackerel, Scomber japonicus.

PubMed

Roldán; Perrotta; Cortey; Pla

2000-10-05

The systematic status and the evolutionary biology of chub mackerel (Scomber japonicus) in the South West Atlantic Ocean is confusing with an unknown degree of genetic differentiation and reproductive isolation between units. Simultaneous genetic and morphologic analyses were made on 227 fish collected from two areas of the South West Atlantic Ocean and one from the Mediterranean Sea. The genetic analysis was based on 36 protein-coding loci, 16 of which were variable. The morphologic analyses include six morphometric length measurements and a meristic character. Correspondence between genetic and morphologic variability patterns indicates isolated Mediterranean and Southwest Atlantic subgroups of S. japonicus and, less clearly, possible additional divergence in two regional stocks within the latter group. The most conservative approach to management is to manage the stocks independently of one another.

31 CFR 129.2 - Definitions.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 31 Money and Finance: Treasury 1 2014-07-01 2014-07-01 false Definitions. 129.2 Section 129.2 Money and Finance: Treasury Regulations Relating to Money and Finance MONETARY OFFICES, DEPARTMENT OF THE TREASURY PORTFOLIO INVESTMENT SURVEY REPORTING § 129.2 Definitions. For purposes of the Act and...

31 CFR 129.2 - Definitions.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 31 Money and Finance: Treasury 1 2013-07-01 2013-07-01 false Definitions. 129.2 Section 129.2 Money and Finance: Treasury Regulations Relating to Money and Finance MONETARY OFFICES, DEPARTMENT OF THE TREASURY PORTFOLIO INVESTMENT SURVEY REPORTING § 129.2 Definitions. For purposes of the Act and...

31 CFR 129.5 - Confidentiality.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 31 Money and Finance: Treasury 1 2010-07-01 2010-07-01 false Confidentiality. 129.5 Section 129.5 Money and Finance: Treasury Regulations Relating to Money and Finance PORTFOLIO INVESTMENT SURVEY REPORTING § 129.5 Confidentiality. (a) Information collected pursuant to the Act will be kept in confidence...

31 CFR 129.1 - Purpose.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 31 Money and Finance: Treasury 1 2013-07-01 2013-07-01 false Purpose. 129.1 Section 129.1 Money and Finance: Treasury Regulations Relating to Money and Finance MONETARY OFFICES, DEPARTMENT OF THE TREASURY PORTFOLIO INVESTMENT SURVEY REPORTING § 129.1 Purpose. The purpose of this part is to provide...

31 CFR 129.1 - Purpose.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 31 Money and Finance: Treasury 1 2014-07-01 2014-07-01 false Purpose. 129.1 Section 129.1 Money and Finance: Treasury Regulations Relating to Money and Finance MONETARY OFFICES, DEPARTMENT OF THE TREASURY PORTFOLIO INVESTMENT SURVEY REPORTING § 129.1 Purpose. The purpose of this part is to provide...

22 CFR 129.9 - Reports.

Code of Federal Regulations, 2011 CFR

2011-04-01

... Directorate of Defense Trade Controls enumerating and describing its brokering activities by quantity, type, U... 22 Foreign Relations 1 2011-04-01 2011-04-01 false Reports. 129.9 Section 129.9 Foreign Relations DEPARTMENT OF STATE INTERNATIONAL TRAFFIC IN ARMS REGULATIONS REGISTRATION AND LICENSING OF BROKERS § 129.9...

22 CFR 129.9 - Reports.

Code of Federal Regulations, 2010 CFR

2010-04-01

... Directorate of Defense Trade Controls enumerating and describing its brokering activities by quantity, type, U... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Reports. 129.9 Section 129.9 Foreign Relations DEPARTMENT OF STATE INTERNATIONAL TRAFFIC IN ARMS REGULATIONS REGISTRATION AND LICENSING OF BROKERS § 129.9...

9 CFR 354.129 - General.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false General. 354.129 Section 354.129 Animals and Animal Products FOOD SAFETY AND INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE AGENCY... Carcasses and Parts § 354.129 General. The carcasses or parts of carcasses of all rabbits inspected at an...

31 CFR 129.2 - Definitions.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 31 Money and Finance: Treasury 1 2010-07-01 2010-07-01 false Definitions. 129.2 Section 129.2 Money and Finance: Treasury Regulations Relating to Money and Finance PORTFOLIO INVESTMENT SURVEY REPORTING § 129.2 Definitions. For purposes of the Act and for reporting requirements under this Part: (a...

31 CFR 129.1 - Purpose.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 31 Money and Finance: Treasury 1 2010-07-01 2010-07-01 false Purpose. 129.1 Section 129.1 Money and Finance: Treasury Regulations Relating to Money and Finance PORTFOLIO INVESTMENT SURVEY REPORTING § 129.1 Purpose. The purpose of this part is to provide general information on portfolio investment...

22 CFR 129.9 - Reports.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 22 Foreign Relations 1 2013-04-01 2013-04-01 false Reports. 129.9 Section 129.9 Foreign Relations DEPARTMENT OF STATE INTERNATIONAL TRAFFIC IN ARMS REGULATIONS REGISTRATION AND LICENSING OF BROKERS § 129.9 Reports. Any person required to register under this part shall provide annually a report to the...

Histological, ultrastructural and heat shock protein 70 (HSP70) responses to heat stress in the sea cucumber Apostichopus japonicus.

PubMed

Xu, Dongxue; Sun, Lina; Liu, Shilin; Zhang, Libin; Yang, Hongsheng

2015-08-01

The aquaculture industry for Apostichopus japonicus has suffered severe economic and resource losses due to high temperature in recent summers. There is increasing concern about the effect of high temperature on this species. Histological, ultrastructural and HSP70 responses to heat stress were investigated in the intestine of A. japonicus. Tissue degradation was observed in muscular, submucosal and mucosal layers, with significant decrease in plicae circulares of the mucosal layer. Ultrastructural damage intensified with increasing stress time, and indicators of cell apoptosis were evident after 192 h heat stress. Immunostaining showed HSP70 mainly in mucosa and serosa, with faint staining in non-stressed individuals (the control group) and denser staining under stress (the 6, 48 and 192 h groups). Western blot detection confirmed ocurrence of HSP70 in all groups and significant up-regulation under stress. The rapid and persistent response of HSP70 implies its critical role in the heat shock response of A. japonicus. Copyright © 2015 Elsevier Ltd. All rights reserved.

Dietary Cerebroside from Sea Cucumber (Stichopus japonicus): Absorption and Effects on Skin Barrier and Cecal Short-Chain Fatty Acids.

PubMed

Duan, Jingjing; Ishida, Marina; Aida, Kazuhiko; Tsuduki, Tsuyoshi; Zhang, Jin; Manabe, Yuki; Hirata, Takashi; Sugawara, Tatsuya

2016-09-21

Sphingolipids from marine sources have attracted more attention recently because of their distinctive structures and expected functions. In this study, the content and components of cerebroside from sea cucumber Stichopus japonicus were analyzed. The absorption of cerebroside from S. japonicus was investigated with an in vivo lipid absorption assay. The result revealed that S. japonicus is a rich source of cerebroside that contained considerable amounts of odd carbon chain sphingoid bases. The cumulative recoveries of d17:1- and d19:2-containing cerebrosides were 0.31 ± 0.16 and 0.32 ± 0.10%, respectively, for 24 h after administration. To the best of the authors' knowledge, this is the first work that shows sphingolipids from a marine source could be absorbed in vivo and incorporated into ceramides. In addition, dietary supplementation with sea cucumber cerebroside to hairless mouse improved the skin barrier function and increased short-chain fatty acids in cecal contents, which have shown beneficial effects on the host.

Morphological and molecular characterization of a microsporidian parasite, Takaokaspora nipponicus n. gen., n. sp. from the invasive rock pool mosquito, Ochlerotatus japonicus japonicus.

PubMed

Andreadis, Theodore G; Takaoka, Hiroyuki; Otsuka, Yasushi; Vossbrinck, Charles R

2013-10-01

A new genus and species of Microsporidia, Takaokaspora nipponicus n. gen., n. sp. is described from Ochlerotatus japonicus japonicus (Theobald) and Ochlerotatus hatorii (Yamada) based on light microscope and ultrastructural morphology, developmental features, transmission cycles and comparative sequence analyses of the small subunit ribosomal DNA (SSU rDNA). The microsporidium is both vertically and horizontally transmitted, exhibits dimorphic development alternating between diplokaryotic and monokaryotic stages and produces two morphologically distinct spores, one in larvae and another in adult females. Horizontal transmission of infection to larval mosquitoes occurs via direct oral ingestion of uninucleate spores that are produced in vertically-infected larval hosts. Development in horizontally-infected hosts is diplokaryotic following karyokinesis of uninucleate schizonts and binary fission to produce small (4.3μm × 2.0μm) membrane free, ovoid, binucleate spores that are confined to adult female reproductive tissues (ovariole sheath and oviducts). Vertical transmission of the microsporidium from adult females to larval progeny takes place via surface contamination of the egg (transovum). Microsporidian development in vertically-infected larvae is haplophasic with unpaired nuclei throughout, producing rosette-shaped sporogonial plasmodia contained within a thin non-persistent sporophorous vesicle and culminating in the formation of membrane free, uninucleate, conical spores (7.0μm×2.8μm). Development is confined to host fat body tissue which appears as swollen white masses in the thorax and selected segments of the abdomen causing larvae to appear abnormally distorted and results in death during the third and fourth instar stages. The SSU rDNA sequences obtained from the two morphologically identical microsporidia isolated from Oc. j. japonicus and Oc. hatorii were nearly identical and unique when compared with GenBank entries of all other mosquito

46 CFR 129.353 - Battery categories.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 4 2010-10-01 2010-10-01 false Battery categories. 129.353 Section 129.353 Shipping... INSTALLATIONS Power Sources and Distribution Systems § 129.353 Battery categories. This section applies to batteries installed to meet the requirements of § 129.310(a) for secondary sources of power to vital loads...

46 CFR 129.353 - Battery categories.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 4 2011-10-01 2011-10-01 false Battery categories. 129.353 Section 129.353 Shipping... INSTALLATIONS Power Sources and Distribution Systems § 129.353 Battery categories. This section applies to batteries installed to meet the requirements of § 129.310(a) for secondary sources of power to vital loads...

46 CFR 129.353 - Battery categories.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 4 2012-10-01 2012-10-01 false Battery categories. 129.353 Section 129.353 Shipping... INSTALLATIONS Power Sources and Distribution Systems § 129.353 Battery categories. This section applies to batteries installed to meet the requirements of § 129.310(a) for secondary sources of power to vital loads...

46 CFR 129.353 - Battery categories.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 4 2013-10-01 2013-10-01 false Battery categories. 129.353 Section 129.353 Shipping... INSTALLATIONS Power Sources and Distribution Systems § 129.353 Battery categories. This section applies to batteries installed to meet the requirements of § 129.310(a) for secondary sources of power to vital loads...

46 CFR 129.353 - Battery categories.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 4 2014-10-01 2014-10-01 false Battery categories. 129.353 Section 129.353 Shipping... INSTALLATIONS Power Sources and Distribution Systems § 129.353 Battery categories. This section applies to batteries installed to meet the requirements of § 129.310(a) for secondary sources of power to vital loads...

22 CFR 129.10 - Reports.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 22 Foreign Relations 1 2014-04-01 2014-04-01 false Reports. 129.10 Section 129.10 Foreign... BROKERS § 129.10 Reports. (a) Any person required to register under this part (including those registered... annual basis a report of its brokering activities in the previous twelve months. Such report shall be...

40 CFR 129.5 - Compliance.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 21 2010-07-01 2010-07-01 false Compliance. 129.5 Section 129.5... STANDARDS Toxic Pollutant Effluent Standards and Prohibitions § 129.5 Compliance. (a)(1) Within 60 days from... standard established for any particular pollutant. (d)(1) Upon the compliance date for any section 307(a...

40 CFR 129.5 - Compliance.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 22 2011-07-01 2011-07-01 false Compliance. 129.5 Section 129.5... STANDARDS Toxic Pollutant Effluent Standards and Prohibitions § 129.5 Compliance. (a)(1) Within 60 days from... standard established for any particular pollutant. (d)(1) Upon the compliance date for any section 307(a...

Determination of triterpene glycosides in sea cucumber (Stichopus japonicus) and its related products by high-performance liquid chromatography.

PubMed

Dong, Ping; Xue, Chang-Hu; Yu, Lin-Fang; Xu, Jie; Chen, Shi-Guo

2008-07-09

A creative and sensitive method has been developed for the determination of triterpene glycosides concentrations in sea cucumber ( Stichopus japonicus) and related products by using d-quinovose (6-deoxyglucose) as the measurement standard by reverse-phase high-performance liquid chromatography (HPLC) and variable-wavelength detection. d-quinovose, which is a unique monosaccharide in holostane triterpene glycosides, was liberated by acid hydrolysis and precolumn derivatized by 1-phenyl-3-methyl-5-pyrazolone (PMP). PMP-quinovose was analyzed by HPLC with 22% acetonitrile in 0.05 M KH2PO4 aquatic solution (pH 5.2) as mobile phase. The calibration curves of d-quinovose were linear within the range of 6.56-164 mg/L (r(2) > 0.995). The contents of triterpene glycosides in various S. japonicus products were determined after appropriate pretreatment methods. The concentration of triterpene glycosides was calculated by the formula C = C(qui) x alpha (alpha = 8.5). The result showed that this method was a simple, rapid, and stable method for the determination of triterpene glycosides in S. japonicus products.

Effects of water depth and substrate color on the growth and body color of the red sea cucumber, Apostichopus japonicus

NASA Astrophysics Data System (ADS)

Jiang, Senhao; Dong, Shuanglin; Gao, Qinfeng; Ren, Yichao; Wang, Fang

2015-05-01

Three color variants of the sea cucumber, Apostichopus japonicus are recognized, the red one is highly valued in the market. When the red variant is cultured in ponds in China, its body color changes from red to celadon in 3-6 months. The effects of water depth and substrate color on the growth and body color of this animal were investigated. Juveniles of red A. japonicus were cultured in cages suspended at a range of water depths (20, 50, 100, 150 and 200 cm). The specific growth rate of red sea cucumbers was significantly higher in animals cultured at deeper water layers compared with those grown at shallowers. Body weights were greatest for sea cucumbers cultured at a depth of 150 cm and their survival rates were highest at a depth of 200 cm. A scale to evaluate the color of red sea cucumbers ( R value) was developed using a Pantone standard color card. All stocked animals in the 9-month trial retained a red color, however the red body color was much more intense in sea cucumbers cultured at shallower depths, while animals suspended in deeper layers became pale. In a separate trial, A. japonicus were cultured in suspended cages with seven different colored substrates. Substrate color had a significant effect on the growth and body-color of red A. japonicus. The yield were greatest for A. japonicus cultured on a yellow substrate, followed by green > white > orange > red > black and blue. All sea cucumbers in the 7-month trial retained a red color, although the red was most intense (highest R value) in animals cultured on a blue substrate and pale (lowest R value) for animals cultured on a green substrate.

Selection of reference genes for qRT-PCR analysis of gene expression in sea cucumber Apostichopus japonicus during aestivation

NASA Astrophysics Data System (ADS)

Zhao, Ye; Chen, Muyan; Wang, Tianming; Sun, Lina; Xu, Dongxue; Yang, Hongsheng

2014-11-01

Quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) is a technique that is widely used for gene expression analysis, and its accuracy depends on the expression stability of the internal reference genes used as normalization factors. However, many applications of qRT-PCR used housekeeping genes as internal controls without validation. In this study, the expression stability of eight candidate reference genes in three tissues (intestine, respiratory tree, and muscle) of the sea cucumber Apostichopus japonicus was assessed during normal growth and aestivation using the geNorm, NormFinder, delta CT, and RefFinder algorithms. The results indicate that the reference genes exhibited significantly different expression patterns among the three tissues during aestivation. In general, the β-tubulin (TUBB) gene was relatively stable in the intestine and respiratory tree tissues. The optimal reference gene combination for intestine was 40S ribosomal protein S18 (RPS18), TUBB, and NADH dehydrogenase (NADH); for respiratory tree, it was β-actin (ACTB), TUBB, and succinate dehydrogenase cytochrome B small subunit (SDHC); and for muscle it was α-tubulin (TUBA) and NADH dehydrogenase [ubiquinone] 1 α subcomplex subunit 13 (NDUFA13). These combinations of internal control genes should be considered for use in further studies of gene expression in A. japonicus during aestivation.

The effects of dietary lead on growth, bioaccumulation and antioxidant capacity in sea cucumber, Apostichopus japonicus.

PubMed

Wang, Jing; Ren, Tongjun; Han, Yuzhe; Zhao, Yang; Liao, Mingling; Wang, Fuqiang; Jiang, Zhiqiang

2015-09-01

Three different diets amended with lead nitrate [Pb(NO3)2] (100, 500 and 1000mg Pb/kg dry weight) and a Pb-free control diet (1.03mg Pb/kg dry weight) were fed to sea cucumber (Apostichopus japonicus) for 30 days. The patterns of Pb accumulation over time were determined in various tissues (body wall, intestine and respiratory tree), as well as growth performance and antioxidant enzymes activities. Pb accumulation in body wall and intestine increased with time in all dietary Pb treatments. When fed the highest Pb diet, the body wall exhibited the greatest Pb burden (16.37mg Pb/kg tissue wet weight), while Pb content in the intestine (2.68mg Pb/kg tissue wet weight) and the respiratory tree (1.78mg Pb/kg tissue wet weight) were lower than Pb content in the body wall by day 30. The body weight gain (BWG), specific growth rate (SGR) and survival rate (SR) had not been affected by 30 days oral administration of Pb supplemented diet. However, the antioxidant enzymes activities [superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px)] of test groups were lower than control group in body wall and malondialdehyde (MDA) concentration in the body wall was opposite after 30 days in sea cucumbers. In summary, this work reports toxic effects in sea cucumber, A. japonicus, after dietary exposure to Pb. Copyright © 2015 Elsevier B.V. All rights reserved.

La Crosse Encephalitis Virus Infection in Field-Collected Aedes albopictus, Aedes japonicus, and Aedes triseriatus in Tennessee.

PubMed

Westby, Katie M; Fritzen, Charissa; Paulsen, Dave; Poindexter, Stephanie; Moncayo, Abelardo C

2015-09-01

La Crosse virus (LACV) is a mosquito-borne virus and a major cause of pediatric encephalitis in the USA. La Crosse virus emerged in Tennessee and other states in the Appalachian region in 1997. We investigated LACV infection rates and seasonal abundances of the native mosquito vector, Aedes triseriatus, and 2 recently introduced mosquito species, Ae. albopictus and Ae. japonicus, in an emerging disease focus in Tennessee. Mosquitoes were collected using multiple trapping methods specific for Aedes mosquitoes at recent human case sites. Mosquito pools were tested via reverse transcriptase-polymerase chain reaction (RT-PCR) of the S segment to detect multiple Bunyamwera and California serogroup viruses, including LACV, as well as real-time RT-PCR of the M segment. A total of 54 mosquito pools were positive, including wild-caught adult females and laboratory-reared adults, demonstrating transovarial transmission in all 3 species. Maximum likelihood estimates (per 1,000 mosquitoes) were 2.72 for Ae. triseriatus, 3.01 for Ae. albopictus, and 0.63 for Ae. japonicus. We conclude that Ae. triseriatus and Ae. albopictus are important LACV vectors and that Ae. japonicus also may be involved in virus maintenance and transmission.

Growth, metabolism and physiological response of the sea cucumber, Apostichopus japonicus Selenka during periods of inactivity

NASA Astrophysics Data System (ADS)

Du, Rongbin; Zang, Yuanqi; Tian, Xiangli; Dong, Shuanglin

2013-03-01

The growth, metabolism and physiological response of the sea cucumber, Apostichopus japonicus, were investigated during periods of inactivity. The body weight, oxygen consumption rate (OCR), activities of acidic phosphatase (ACP), alkaline phosphatase (AKP), catalase (CAT) and superoxide dismutase (SOD), and content of heat shock protein 70 (Hsp70) in the body wall and coelomic fluid of A. japonicus were measured during starvation, experimental aestivation and aestivation. The results showed that the body weight of sea cucumber in the three treatments decreased significantly during the experimental period ( P < 0.05). The OCR of sea cucumber reduced in starvation and experimental aestivation treatments, but increased gradually in natural aestivation treatment. The activities of ACP and AKP of sea cucumber decreased gradually in all treatments, whereas those of SOD and CAT as well as Hsp70 content decreased in the starvation and experimental aestivation treatments and increased in natural aestivation treatment. The sea cucumber entered a state of aestivation at 24°C. To some extent, the animals in experimental aestivation were different from those in natural aestivation in metabolism and physiological response. These findings suggested that the aestivation mechanism of A. japonicus is complex and may not be attributed to the elevated temperature only.

Soluble epoxide hydrolase inhibitory activity of components from Leonurus japonicus.

PubMed

Leem, Hyun Hee; Lee, Ga Young; Lee, Ji Sun; Lee, Hanna; Kim, Jang Hoon; Kim, Young Ho

2017-10-01

One new compound, 10-methoxy-leonurine (1), and four known compounds (2-5) were purified by silica gel, C-18, and Sephadex LH-20 column chromatography from Leonurus japonicus. Their structures were elucidated using one-dimensional (1D)/two-dimensional (2D)-nuclear magnetic resonance (NMR), high-resolution (HR)-electrospray ionization (ESI) mass spectrometry (MS). The compounds were evaluated to determine their inhibition of the catalysis of soluble epoxide hydrolase (sEH). According to the results from in vitro analyses, compounds 1 and 2, which contain guanidine and flavonoid (3), were determined to be potential inhibitors of this enzyme. All compounds were revealed to be non-competitive inhibitors according to Lineweaver-Burk plots. Furthermore, in silico molecular docking indicated that compounds 1-3 are bound to sEH in a similar fashion and have stable binding energies, as calculated by AutoDock 4.2. Molecular dynamics determined the root-mean-square deviation (RMSD), total energy, RMS fluctuation (RMSF), hydrogen bonds, and distance of the complex according to time. Copyright © 2017 Elsevier B.V. All rights reserved.

Results of a Prospective Study of Acute Coronary Syndrome Hospitalization After Enactment of a Smoking Ban in Public Places in Hyogo Prefecture　- Comparison With Gifu, a Prefecture Without a Public Smoking Ban.

PubMed

Sato, Yukihito; Minatoguchi, Shinya; Nishigaki, Kazuhiko; Hirata, Ken-Ichi; Masuyama, Tohru; Furukawa, Yutaka; Uematsu, Masaaki; Yoshikawa, Junichi; Otsuji, Satoru; Iida, Mami; Fujiwara, Hisayoshi

2016-11-25

Hyogo Prefecture is the 2nd prefecture in Japan, after Kanagawa, to enact a ban with penal code on smoking in public places, although the restriction is partial.Methods and Results:This study included consecutive patients with acute coronary syndrome (ACS) who were admitted to 33 major hospitals in the Hyogo District during the 12 months before implementation of the legislation and during the 24 months thereafter. Consecutive patients with ACS from Gifu Prefecture who were admitted to 20 major hospitals were enrolled as geographical controls. The number of ACS admissions did not change from the years 2012-2015 in both Hyogo District (1,774 in the pre-year, 1,784 in the 1st year, and 1,720 in the 2nd year) and Gifu Prefecture (1,226 in the pre-year, 1,174 in the 1st year, and 1,206 in the 2nd year). However, a clear reduction was observed in the subanalysis for Kobe City (895 in the preceding year, 830 (-7.3%) in the 1st year, and 792 (-11.5%) in the 2nd year), where adherence to the smoking ban was higher than in other Hyogo districts. The primary endpoint did not show a significant change. However, the subanalysis showed a significant decrease in ACS admissions in Kobe City. These results suggest that ACS reduction may depend on the degree of adherence to a smoking ban. (Circ J 2016; 80: 2528-2532).

Antioxidation activities of low-molecular-weight gelatin hydrolysate isolated from the sea cucumber Stichopus japonicus

NASA Astrophysics Data System (ADS)

Wang, Jingfeng; Wang, Yuming; Tang, Qingjuan; Wang, Yi; Chang, Yaoguang; Zhao, Qin; Xue, Changhu

2010-03-01

Gelatin extracted from the body wall of the sea cucumber ( Stichopus japonicus) was hydrolyzed with flavourzyme. Low-molecular-weight gelatin hydrolysate (LMW-GH) of 700-1700 Da was produced using an ultrafiltration membrane bioreactor system. Chemiluminescence analysis revealed that LMW-GH scavenges high free radicals in a concentration-dependent manner; IC50 value for superoxide and hydroxyl radicals was 442 and 285 μg mL-1, respectively. LMW-GH exhibited excellent inhibitory characteristics against melanin synthesis and tyrosinase activity in B16 cells. Furthermore, LMW-GH notably increased intracellular glutathione (GSH), which in turn suppressed melanogenesis. LMW-GH performs antioxidation activity, holding the potential of being used as a valuable ingredient in function foods, cosmetics and pharmaceuticals or nutriceuticals.

40 CFR 86.129-80 - Road load power, test weight, and inertia weight class determination.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 40 Protection of Environment 19 2014-07-01 2014-07-01 false Road load power, test weight, and inertia weight class determination. 86.129-80 Section 86.129-80 Protection of Environment ENVIRONMENTAL... power, test weight, and inertia weight class determination. (a) [Reserved] (b) Power absorption unit...

7 CFR 4280.129 - Evaluation of guaranteed loan applications.

Code of Federal Regulations, 2011 CFR

2011-01-01

...-COOPERATIVE SERVICE AND RURAL UTILITIES SERVICE, DEPARTMENT OF AGRICULTURE LOANS AND GRANTS Renewable Energy Systems and Energy Efficiency Improvements Program Section B. Guaranteed Loans § 4280.129 Evaluation of... both the borrower and project are eligible, the project has technical merit, there is reasonable...

7 CFR 4280.129 - Evaluation of guaranteed loan applications.

Code of Federal Regulations, 2010 CFR

2010-01-01

...-COOPERATIVE SERVICE AND RURAL UTILITIES SERVICE, DEPARTMENT OF AGRICULTURE LOANS AND GRANTS Renewable Energy Systems and Energy Efficiency Improvements Program Section B. Guaranteed Loans § 4280.129 Evaluation of... both the borrower and project are eligible, the project has technical merit, there is reasonable...

46 CFR 129.315 - Power sources for OSVs of 100 or more gross tons.

Code of Federal Regulations, 2013 CFR

2013-10-01

... VESSELS ELECTRICAL INSTALLATIONS Power Sources and Distribution Systems § 129.315 Power sources for OSVs... 46 Shipping 4 2013-10-01 2013-10-01 false Power sources for OSVs of 100 or more gross tons. 129....10 of this chapter. (b) If a generator provides electrical power for any system identified as a vital...

46 CFR 129.315 - Power sources for OSVs of 100 or more gross tons.

Code of Federal Regulations, 2011 CFR

2011-10-01

... VESSELS ELECTRICAL INSTALLATIONS Power Sources and Distribution Systems § 129.315 Power sources for OSVs... 46 Shipping 4 2011-10-01 2011-10-01 false Power sources for OSVs of 100 or more gross tons. 129....10 of this chapter. (b) If a generator provides electrical power for any system identified as a vital...

46 CFR 129.315 - Power sources for OSVs of 100 or more gross tons.

Code of Federal Regulations, 2012 CFR

2012-10-01

... VESSELS ELECTRICAL INSTALLATIONS Power Sources and Distribution Systems § 129.315 Power sources for OSVs... 46 Shipping 4 2012-10-01 2012-10-01 false Power sources for OSVs of 100 or more gross tons. 129....10 of this chapter. (b) If a generator provides electrical power for any system identified as a vital...

Whole-nerve chorda tympani responses to sweeteners in C57BL/6ByJ and 129P3/J mice

PubMed Central

Inoue, Masashi; McCaughey, Stuart A.; Bachmanov, Alexander A.; Beauchamp, Gary K.

2013-01-01

The C57BL/6ByJ (B6) strain of mice exhibits higher preferences than does the 129P3/J (129) strain for a variety of sweet-tasting compounds. We measured gustatory afferent responses of the whole chorda tympani nerve in these two strains using a broad array of sweeteners and other taste stimuli. Neural responses were greater in B6 than in 129 mice to the sugars sucrose and maltose, the polyol D-sorbitol, and the non-caloric sweeteners NaSaccharin, acesulfame-K, SC-45647, and sucralose. Lower neural response thresholds were also observed in the B6 strain for most of these stimuli. The strains did not differ on their neural responses to amino acids that are thought to taste sweet to mice, with the exception of L-proline, which evoked larger responses in the B6 strain. Aspartame and thaumatin, which taste sweet to humans but are not strongly preferred by B6 or 129 mice, did not evoke neural responses that exceeded threshold in either strain. The strains generally did not differ in their neural responses to NaCl, quinine, and HCl. Thus, variation between the B6 and 129 strains in the peripheral gustatory system may contribute to differences in their consumption of many sweeteners. PMID:11555486

Phylogeography of the sandy beach amphipod Haustorioides japonicus along the Sea of Japan: Paleogeographical signatures of cryptic regional divergences

NASA Astrophysics Data System (ADS)

Takada, Yoshitake; Sakuma, Kay; Fujii, Tetsuo; Kojima, Shigeaki

2018-01-01

Recent findings of genetic breaks within apparently continuous marine populations challenge the traditional vicariance paradigm in population genetics. Such "invisible" boundaries are sometimes associated with potential geographic barriers that have forced divergence of an ancestral population, habitat discontinuities, biogeographic disjunctions due to environmental gradients, or a combination of these factors. To explore the factors that influence the genetic population structure of apparently continuous populations along the Sea of Japan, the sandy beach amphipod Haustorioides japonicus was examined. We sampled a total of 300 individuals of H. japonicus from the coast of Japan, and obtained partial sequences of the mitochondrial COI gene. The sequences from 19 local populations were clustered into five groups (Northwestern Pacific, Northern, Central, Southern Sea of Japan, and East China Sea) based on a spatial genetic mixture analysis and a minimum-spanning network. AMOVA and pairwise Fst tests further supported the significant divergence of the five groups. Phylogenetic analysis revealed the relationship among the haplotypes of H. japonicus and outgroups, which inferred the northward range expansion of the species. A relaxed molecular-clock Bayesian analysis inferred the early-to middle-Pleistocene divergence of the populations. Among the five clusters, the Central Sea of Japan showed the highest values for genetic diversity indices indicating the existence of a relatively stable and large population there. The hypothesis is also supported by Bayesian Skyline Plots that showed sudden population expansion for all the clusters except for Central Sea of Japan. The present study shows genetic boundaries between the Sea of Japan and the neighboring seas, probably due to geographic isolation during the Pleistocene glacial periods. We further found divergence between the populations along the apparently continuous coast of the Sea of Japan. Historical changes in the

Iron-induced nitric oxide leads to an increase in the expression of ferritin during the senescence of Lotus japonicus nodules.

PubMed

Chungopast, Sirinapa; Duangkhet, Mallika; Tajima, Shigeyuki; Ma, Jian Feng; Nomura, Mika

2017-01-01

Iron is an essential nutrient for legume-rhizobium symbiosis and accumulates abundantly in the nodules. However, the concentration of free iron in the cells is strictly controlled to avoid toxicity. It is known that ferritin accumulates in the cells as an iron storage protein. During nodule senescence, the expression of the ferritin gene, Ljfer1, was induced in Lotus japonicus. We investigated a signal transduction pathway leading to the increase of Ljfer1 in the nodule. The Ljfer1 promoter of L. japonicus contains a conserved Iron-Dependent Regulatory Sequence (IDRS). The expression of Ljfer1 was induced by the application of iron or sodium nitroprusside, which is a nitric oxide (NO) donor. The application of iron to the nodule increased the level of NO. These data strongly suggest that iron-induced NO leads to increased expression of Ljfer1 during the senescence of L. japonicus nodules. Copyright © 2016 Elsevier GmbH. All rights reserved.

Cell wall polysaccharides released during the alcoholic fermentation by Schizosaccharomyces pombe and S. japonicus: quantification and characterization

PubMed Central

Domizio, P.; Liu, Y.; Bisson, L.F.; Barile, D.

2016-01-01

The present work demonstrates that yeasts belonging to the Schizosaccharomyces genus release a high quantity of polysaccharides of cell wall origin starting from the onset of the alcoholic fermentation. By the end of the alcoholic fermentation, all of the Schizosaccharomyces yeast strains released a quantity of polysaccharides approximately 3-7 times higher than that released by a commercial Saccharomyces cerevisiae yeast strain under the same fermentative conditions of synthetic juice. A higher content of polysaccharide was found in media fermented by Schizosaccharomyces japonicus with respect to that of Schizosaccharomyces pombe. Some of the strains evaluated were also able to produce high levels of pyruvic acid, which has been shown to be an important compound for color stability of wine. The presence of strains with different malic acid consumption patterns along with high polysaccharide release would enable production of naturally modified wines with enhanced mouth feel and reduced acidity. The chemical analysis of the released polysaccharides demonstrated divergence between the two yeast species S. pombe and S. japonicus. A different mannose/galactose ratio and a different percentage of proteins was observed on the polysaccharides released by S. pombe as compared to S. japonicus. Analysis of the proteins released in the media revealed the presence of a glycoprotein with a molecular size around 32-33 kDa only for the species S. japonicus. Mass spectrometry analysis of carbohydrate moieties showed similar proportions among the N-glycan chains released in the media by both yeast species but differences between the two species were also observed. These observations suggest a possible role of rapid MALDI-TOF screening of N-glycans compositional fingerprint as a taxonomic tool for this genus. Polysaccharides release in the media, in particular galactomannoproteins in significant amounts, could make these yeasts particularly interesting also for the industrial

Fungal biodegradation and biotransformation of soluble lignocarbohydrate complexes from straw. [Aspergillus japonicus, Polyporus versicolor

DOE Office of Scientific and Technical Information (OSTI.GOV)

Milstein, O.A.; Vared, Y.; Sharma, A.

1983-08-01

Aspergillus japonicus is an efficient degrader of phenolics and carbohydrates present in a mixture of soluble lignocarbohydrate complexes extracted from wheat straw. Trichoderma species attacked part of the carbohydrate but hardly affected the aromatic portion of this solution. Polyporus versicolor had a complex effect; polymerization of low-molecular-size phenolics accompanied the degradation of aromatic and carbohydrate polymers. The addition of xylose to the medium facilitated depolymerization of lignin by the fungi tested and prevented the polymerization of low-molecular-size fractions of lignocarbohydrate complexes by P. versicolor. P. versicolor, in contrast to A. japonicus and Trichoderma species, also excreted into the medium considerablemore » amounts of laccase, but only in the absence of endogenous or exogenous carbohydrates. Apparently, laccase is involved in polymerization rather than degradation of lignin in this organism. A number of extracellular glycanases were also secreted by these fungi. 19 references« less

14 CFR 65.129 - Performance standards.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 14 Aeronautics and Space 2 2013-01-01 2013-01-01 false Performance standards. 65.129 Section 65.129 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRMEN CERTIFICATION: AIRMEN OTHER THAN FLIGHT CREWMEMBERS Parachute Riggers § 65.129 Performance...

14 CFR 65.129 - Performance standards.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 14 Aeronautics and Space 2 2012-01-01 2012-01-01 false Performance standards. 65.129 Section 65.129 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRMEN CERTIFICATION: AIRMEN OTHER THAN FLIGHT CREWMEMBERS Parachute Riggers § 65.129 Performance...

14 CFR 65.129 - Performance standards.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 14 Aeronautics and Space 2 2014-01-01 2014-01-01 false Performance standards. 65.129 Section 65.129 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRMEN CERTIFICATION: AIRMEN OTHER THAN FLIGHT CREWMEMBERS Parachute Riggers § 65.129 Performance...

14 CFR 65.129 - Performance standards.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 2 2011-01-01 2011-01-01 false Performance standards. 65.129 Section 65.129 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRMEN CERTIFICATION: AIRMEN OTHER THAN FLIGHT CREWMEMBERS Parachute Riggers § 65.129 Performance...

14 CFR 65.129 - Performance standards.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 2 2010-01-01 2010-01-01 false Performance standards. 65.129 Section 65.129 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRMEN CERTIFICATION: AIRMEN OTHER THAN FLIGHT CREWMEMBERS Parachute Riggers § 65.129 Performance...

Genetic Screening Identifies Cyanogenesis-Deficient Mutants of Lotus japonicus and Reveals Enzymatic Specificity in Hydroxynitrile Glucoside Metabolism[W][OA

PubMed Central

Takos, Adam; Lai, Daniela; Mikkelsen, Lisbeth; Abou Hachem, Maher; Shelton, Dale; Motawia, Mohammed Saddik; Olsen, Carl Erik; Wang, Trevor L.; Martin, Cathie; Rook, Fred

2010-01-01

Cyanogenesis, the release of hydrogen cyanide from damaged plant tissues, involves the enzymatic degradation of amino acid–derived cyanogenic glucosides (α-hydroxynitrile glucosides) by specific β-glucosidases. Release of cyanide functions as a defense mechanism against generalist herbivores. We developed a high-throughput screening method and used it to identify cyanogenesis deficient (cyd) mutants in the model legume Lotus japonicus. Mutants in both biosynthesis and catabolism of cyanogenic glucosides were isolated and classified following metabolic profiling of cyanogenic glucoside content. L. japonicus produces two cyanogenic glucosides: linamarin (derived from Val) and lotaustralin (derived from Ile). Their biosynthesis may involve the same set of enzymes for both amino acid precursors. However, in one class of mutants, accumulation of lotaustralin and linamarin was uncoupled. Catabolic mutants could be placed in two complementation groups, one of which, cyd2, encoded the β-glucosidase BGD2. Despite the identification of nine independent cyd2 alleles, no mutants involving the gene encoding a closely related β-glucosidase, BGD4, were identified. This indicated that BGD4 plays no role in cyanogenesis in L. japonicus in vivo. Biochemical analysis confirmed that BGD4 cannot hydrolyze linamarin or lotaustralin and in L. japonicus is specific for breakdown of related hydroxynitrile glucosides, such as rhodiocyanoside A. By contrast, BGD2 can hydrolyze both cyanogenic glucosides and rhodiocyanosides. Our genetic analysis demonstrated specificity in the catabolic pathways for hydroxynitrile glucosides and implied specificity in their biosynthetic pathways as well. In addition, it has provided important tools for elucidating and potentially modifying cyanogenesis pathways in plants. PMID:20453117

46 CFR 129.370 - Equipment grounding.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 4 2012-10-01 2012-10-01 false Equipment grounding. 129.370 Section 129.370 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OFFSHORE SUPPLY VESSELS ELECTRICAL INSTALLATIONS Power Sources and Distribution Systems § 129.370 Equipment grounding. (a) On a metallic vessel...

46 CFR 129.370 - Equipment grounding.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 4 2014-10-01 2014-10-01 false Equipment grounding. 129.370 Section 129.370 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OFFSHORE SUPPLY VESSELS ELECTRICAL INSTALLATIONS Power Sources and Distribution Systems § 129.370 Equipment grounding. (a) On a metallic vessel...

46 CFR 129.370 - Equipment grounding.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 4 2013-10-01 2013-10-01 false Equipment grounding. 129.370 Section 129.370 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OFFSHORE SUPPLY VESSELS ELECTRICAL INSTALLATIONS Power Sources and Distribution Systems § 129.370 Equipment grounding. (a) On a metallic vessel...

47 CFR 101.129 - Transmitter location.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 47 Telecommunication 5 2011-10-01 2011-10-01 false Transmitter location. 101.129 Section 101.129 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) SAFETY AND SPECIAL RADIO SERVICES FIXED MICROWAVE SERVICES Technical Standards § 101.129 Transmitter location. (a) The applicant must determine, prior to...

47 CFR 101.129 - Transmitter location.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 47 Telecommunication 5 2012-10-01 2012-10-01 false Transmitter location. 101.129 Section 101.129 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) SAFETY AND SPECIAL RADIO SERVICES FIXED MICROWAVE SERVICES Technical Standards § 101.129 Transmitter location. (a) The applicant must determine, prior to...

45 CFR 12.9 - General disposal terms and conditions.

Code of Federal Regulations, 2014 CFR

2014-10-01

... UTILIZATION OF SURPLUS REAL PROPERTY FOR PUBLIC HEALTH PURPOSES § 12.9 General disposal terms and conditions. (a) Surplus real property transfers under this part will be limited to public health purposes... hereof. (b) A transfer of surplus real property for public health purposes is subject to the disapproval...

45 CFR 12.9 - General disposal terms and conditions.

Code of Federal Regulations, 2013 CFR

2013-10-01

... UTILIZATION OF SURPLUS REAL PROPERTY FOR PUBLIC HEALTH PURPOSES § 12.9 General disposal terms and conditions. (a) Surplus real property transfers under this part will be limited to public health purposes... hereof. (b) A transfer of surplus real property for public health purposes is subject to the disapproval...

45 CFR 12.9 - General disposal terms and conditions.

Code of Federal Regulations, 2012 CFR

2012-10-01

... UTILIZATION OF SURPLUS REAL PROPERTY FOR PUBLIC HEALTH PURPOSES § 12.9 General disposal terms and conditions. (a) Surplus real property transfers under this part will be limited to public health purposes... hereof. (b) A transfer of surplus real property for public health purposes is subject to the disapproval...

Role of crustacean hyperglycemic hormone (CHH) in the environmental stressor-exposed intertidal copepod Tigriopus japonicus.

PubMed

Kim, Bo-Mi; Jeong, Chang-Bum; Han, Jeonghoon; Kim, Il-Chan; Rhee, Jae-Sung; Lee, Jae-Seong

2013-09-01

To identify and characterize CHH (TJ-CHH) gene in the copepod Tigriopus japonicus, we analyzed the full-length cDNA sequence, genomic structure, and promoter region. The full-length TJ-CHH cDNA was 716 bp in length, encoding 136 amino acid residues. The deduced amino acid sequences of TJ-CHH showed a high similarity of the CHH mature domain to other crustaceans. Six conserved cysteine residues and five conserved structural motifs in the CHH mature peptide domain were also observed. The genomic structure of the TJ-CHH gene contained three exons and two introns in its open reading frame (ORF), and several transcriptional elements were detected in the promoter region of the TJ-CHH gene. To investigate transcriptional change of TJ-CHH under environmental stress, T. japonicus were exposed to heat treatment, UV-B radiation, heavy metals, and water-accommodated fractions (WAFs) of Iranian crude oil. Upon heat stress, TJ-CHH transcripts were elevated at 30 °C and 35 °C for 96 h in a time-course experiment. UV-B radiation led to a decreased pattern of the TJ-CHH transcript 48 h and more after radiation (12 kJ/m(2)). After exposure of a fixed dose (12 kJ/m(2)) in a time-course experiment, TJ-CHH transcript was down-regulated in time-dependent manner with a lowest value at 12h. However, the TJ-CHH transcript level was increased in response to five heavy metal exposures for 96 h. Also, the level of the TJ-CHH transcript was significantly up-regulated at 20% of WAFs after exposure to WAFs for 48 h and then remarkably reduced in a dose-dependent manner. These findings suggest that the enhanced TJ-CHH transcript level is associated with a cellular stress response of the TJ-CHH gene as shown in decapod crustaceans. This study is also helpful for a better understanding of the detrimental effects of environmental changes on the CHH-triggered copepod metabolism. Copyright © 2013 Elsevier Inc. All rights reserved.

46 CFR 129.323 - Multiple generators.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 4 2014-10-01 2014-10-01 false Multiple generators. 129.323 Section 129.323 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OFFSHORE SUPPLY VESSELS ELECTRICAL INSTALLATIONS Power Sources and Distribution Systems § 129.323 Multiple generators. If an OSV uses two or more...

46 CFR 129.323 - Multiple generators.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 4 2013-10-01 2013-10-01 false Multiple generators. 129.323 Section 129.323 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OFFSHORE SUPPLY VESSELS ELECTRICAL INSTALLATIONS Power Sources and Distribution Systems § 129.323 Multiple generators. If an OSV uses two or more...

46 CFR 129.323 - Multiple generators.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 4 2011-10-01 2011-10-01 false Multiple generators. 129.323 Section 129.323 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OFFSHORE SUPPLY VESSELS ELECTRICAL INSTALLATIONS Power Sources and Distribution Systems § 129.323 Multiple generators. If an OSV uses two or more...

46 CFR 129.323 - Multiple generators.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 4 2010-10-01 2010-10-01 false Multiple generators. 129.323 Section 129.323 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OFFSHORE SUPPLY VESSELS ELECTRICAL INSTALLATIONS Power Sources and Distribution Systems § 129.323 Multiple generators. If an OSV uses two or more...

46 CFR 129.323 - Multiple generators.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 4 2012-10-01 2012-10-01 false Multiple generators. 129.323 Section 129.323 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OFFSHORE SUPPLY VESSELS ELECTRICAL INSTALLATIONS Power Sources and Distribution Systems § 129.323 Multiple generators. If an OSV uses two or more...

31 CFR 129.4 - Recordkeeping requirement.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 31 Money and Finance: Treasury 1 2014-07-01 2014-07-01 false Recordkeeping requirement. 129.4 Section 129.4 Money and Finance: Treasury Regulations Relating to Money and Finance MONETARY OFFICES, DEPARTMENT OF THE TREASURY PORTFOLIO INVESTMENT SURVEY REPORTING § 129.4 Recordkeeping requirement. Reporters...

31 CFR 129.4 - Recordkeeping requirement.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 31 Money and Finance: Treasury 1 2013-07-01 2013-07-01 false Recordkeeping requirement. 129.4 Section 129.4 Money and Finance: Treasury Regulations Relating to Money and Finance MONETARY OFFICES, DEPARTMENT OF THE TREASURY PORTFOLIO INVESTMENT SURVEY REPORTING § 129.4 Recordkeeping requirement. Reporters...

31 CFR 129.3 - Reporting requirements.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 31 Money and Finance: Treasury 1 2013-07-01 2013-07-01 false Reporting requirements. 129.3 Section 129.3 Money and Finance: Treasury Regulations Relating to Money and Finance MONETARY OFFICES, DEPARTMENT OF THE TREASURY PORTFOLIO INVESTMENT SURVEY REPORTING § 129.3 Reporting requirements. (a) Notice...

31 CFR 129.3 - Reporting requirements.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 31 Money and Finance: Treasury 1 2014-07-01 2014-07-01 false Reporting requirements. 129.3 Section 129.3 Money and Finance: Treasury Regulations Relating to Money and Finance MONETARY OFFICES, DEPARTMENT OF THE TREASURY PORTFOLIO INVESTMENT SURVEY REPORTING § 129.3 Reporting requirements. (a) Notice...

7 CFR 1955.129 - Business brokers.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 14 2010-01-01 2009-01-01 true Business brokers. 1955.129 Section 1955.129 Agriculture Regulations of the Department of Agriculture (Continued) RURAL HOUSING SERVICE, RURAL BUSINESS... Dispose of Inventory Property § 1955.129 Business brokers. The services of business brokers or business...

29 CFR 452.129 - Non-discrimination.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 29 Labor 2 2014-07-01 2014-07-01 false Non-discrimination. 452.129 Section 452.129 Labor Regulations Relating to Labor OFFICE OF LABOR-MANAGEMENT STANDARDS, DEPARTMENT OF LABOR LABOR-MANAGEMENT... DISCLOSURE ACT OF 1959 Election Procedures; Rights of Members § 452.129 Non-discrimination. Further...

29 CFR 452.129 - Non-discrimination.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 29 Labor 2 2012-07-01 2012-07-01 false Non-discrimination. 452.129 Section 452.129 Labor Regulations Relating to Labor OFFICE OF LABOR-MANAGEMENT STANDARDS, DEPARTMENT OF LABOR LABOR-MANAGEMENT... DISCLOSURE ACT OF 1959 Election Procedures; Rights of Members § 452.129 Non-discrimination. Further...

29 CFR 452.129 - Non-discrimination.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 29 Labor 2 2013-07-01 2013-07-01 false Non-discrimination. 452.129 Section 452.129 Labor Regulations Relating to Labor OFFICE OF LABOR-MANAGEMENT STANDARDS, DEPARTMENT OF LABOR LABOR-MANAGEMENT... DISCLOSURE ACT OF 1959 Election Procedures; Rights of Members § 452.129 Non-discrimination. Further...

29 CFR 452.129 - Non-discrimination.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 29 Labor 2 2010-07-01 2010-07-01 false Non-discrimination. 452.129 Section 452.129 Labor Regulations Relating to Labor OFFICE OF LABOR-MANAGEMENT STANDARDS, DEPARTMENT OF LABOR LABOR-MANAGEMENT... DISCLOSURE ACT OF 1959 Election Procedures; Rights of Members § 452.129 Non-discrimination. Further...

29 CFR 452.129 - Non-discrimination.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 29 Labor 2 2011-07-01 2011-07-01 false Non-discrimination. 452.129 Section 452.129 Labor Regulations Relating to Labor OFFICE OF LABOR-MANAGEMENT STANDARDS, DEPARTMENT OF LABOR LABOR-MANAGEMENT... DISCLOSURE ACT OF 1959 Election Procedures; Rights of Members § 452.129 Non-discrimination. Further...

46 CFR 129.510 - Lifeboat winches.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 4 2014-10-01 2014-10-01 false Lifeboat winches. 129.510 Section 129.510 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OFFSHORE SUPPLY VESSELS ELECTRICAL INSTALLATIONS Miscellaneous Electrical Systems § 129.510 Lifeboat winches. Each lifeboat winch operated by electric power must...

46 CFR 129.510 - Lifeboat winches.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 4 2012-10-01 2012-10-01 false Lifeboat winches. 129.510 Section 129.510 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OFFSHORE SUPPLY VESSELS ELECTRICAL INSTALLATIONS Miscellaneous Electrical Systems § 129.510 Lifeboat winches. Each lifeboat winch operated by electric power must...

31 CFR 129.4 - Recordkeeping requirement.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 31 Money and Finance: Treasury 1 2010-07-01 2010-07-01 false Recordkeeping requirement. 129.4 Section 129.4 Money and Finance: Treasury Regulations Relating to Money and Finance PORTFOLIO INVESTMENT SURVEY REPORTING § 129.4 Recordkeeping requirement. Reporters shall maintain all information used in...

31 CFR 129.3 - Reporting requirements.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 31 Money and Finance: Treasury 1 2010-07-01 2010-07-01 false Reporting requirements. 129.3 Section 129.3 Money and Finance: Treasury Regulations Relating to Money and Finance PORTFOLIO INVESTMENT SURVEY REPORTING § 129.3 Reporting requirements. (a) Notice of specific reporting requirements, including...

First record of Neoergasilus japonicus (Poecilostomatoida: Ergasilidae), a parasitic copepod new to the Laurentian Great Lakes

USGS Publications Warehouse

Hudson, Patrick L.; Bowen, Charles A.

2002-01-01

The parasitic copepod Neoergasilus japonicus, native to eastern Asia, was first collected from 4 species of fish (fathead minnow, Pimephales promelas; largemouth bass, Micropterus salmoides; pumpkinseed sunfish, Lepomis gibbosus; and yellow perch, Perca flavescens) in July 1994 in Saginaw Bay, Lake Huron, Michigan. Further sampling in the bay in 2001 revealed infections on 7 additional species (bluegill, Lepomis macrochirus; carp, Cyprinus carpio; channel catfish, Ictalurus punctatus; goldfish, Carassius auratus; green sunfish, Lepomis cyanellus; rock bass, Ambloplites rupestris; and smallmouth bass, Micropterus dolomieu). An additional 21 species examined in 2001 were devoid of the parasite. A limited collection of fish from Lake Superior (n = 8) and Lake Michigan (n = 46) in 1994 showed no infection. Neoergasilus japonicus is most frequently found attached to the dorsal fin and, in decreasing frequency, on the anal, tail, pelvic, and pectoral fins. Prevalence generally ranged from 15 to 70 and intensity from 1 to 10. The greatest number of copepods on a single host was 44. The copepod Neoergasilus japonicus appears to disperse over long distances rather quickly, spreading across Europe in 20 yr and then moving on to North America over a span of 10 yr. Its main vehicle of transport and introduction into the Great Lakes is probably exotic fish hosts associated with the fish-culture industry.

Real-time PCR Tests in Dutch Exotic Mosquito Surveys; Implementation of Aedes aegypti and Aedes albopictus Identification Tests, and the Development of Tests for the Identification of Aedes atropalpus and Aedes japonicus japonicus (Diptera: Culicidae).

PubMed

van de Vossenberg, B T L H; Ibáñez-Justicia, A; Metz-Verschure, E; van Veen, E J; Bruil-Dieters, M L; Scholte, E J

2015-05-01

Since 2009, The Netherlands Food and Consumer Product Safety Authority carries out surveys focusing on, amongst others, the presence of invasive mosquito species (IMS). Special attention is given to exotic container-breeding Aedes species Aedes aegypti (L.), Aedes albopictus (Skuse), Aedes atropalpus (Coquillett), and Aedes japonicus japonicus (Theobald). This study describes the implementation of real-time PCR tests described by Hill et al. (2008) for the identification of Ae. aegypti and Ae. albopictus, and the development of two novel real-time PCR tests for the identification of Ae. atropalpus and Ae. j. japonicus. Initial test showed that optimization of elements of the Ae. aegypti and Ae. albopictus tests was needed. Method validation tests were performed to determine if the implemented and newly developed tests are fit for routine diagnostics. Performance criteria of analytical sensitivity, analytical specificity, selectivity, repeatability, and reproducibility were determined. In addition, experiments were performed to determine the influence of environmental conditions on the usability of DNA extracted from mosquito specimens trapped in BG-Sentinel traps. The real-time PCR tests were demonstrated to be sensitive, specific, repeatable, reproducible, and are less prone to false negative results compared to partial cytochrome c oxidase I gene sequencing owing to the DNA fragmentation caused by environmental influences. © The Authors 2015. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

46 CFR 129.395 - Radio installations.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 4 2010-10-01 2010-10-01 false Radio installations. 129.395 Section 129.395 Shipping... INSTALLATIONS Power Sources and Distribution Systems § 129.395 Radio installations. A separate circuit, with overcurrent protection at the switchboard, must be provided for at least one radio installation. Additional...

50 CFR 300.129 - Fishing year.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 50 Wildlife and Fisheries 11 2012-10-01 2012-10-01 false Fishing year. 300.129 Section 300.129 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS Vessels of the United States Fishing in Colombian Treaty Waters § 300.129 Fishing year. The fishing year...

50 CFR 300.129 - Fishing year.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 50 Wildlife and Fisheries 11 2014-10-01 2014-10-01 false Fishing year. 300.129 Section 300.129 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS Vessels of the United States Fishing in Colombian Treaty Waters § 300.129 Fishing year. The fishing year...

50 CFR 300.129 - Fishing year.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 50 Wildlife and Fisheries 11 2013-10-01 2013-10-01 false Fishing year. 300.129 Section 300.129 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS Vessels of the United States Fishing in Colombian Treaty Waters § 300.129 Fishing year. The fishing year...

50 CFR 300.129 - Fishing year.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 50 Wildlife and Fisheries 9 2011-10-01 2011-10-01 false Fishing year. 300.129 Section 300.129 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS Vessels of the United States Fishing in Colombian Treaty Waters § 300.129 Fishing year. The fishing year...

50 CFR 300.129 - Fishing year.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 50 Wildlife and Fisheries 7 2010-10-01 2010-10-01 false Fishing year. 300.129 Section 300.129 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS Vessels of the United States Fishing in Colombian Treaty Waters § 300.129 Fishing year. The fishing year...

14 CFR 129.25 - Airplane security.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Airplane security. 129.25 Section 129.25 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIR CARRIERS... AND FOREIGN OPERATORS OF U.S.-REGISTERED AIRCRAFT ENGAGED IN COMMON CARRIAGE General § 129.25 Airplane...

14 CFR 21.129 - Tests: propellers.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 14 Aeronautics and Space 1 2012-01-01 2012-01-01 false Tests: propellers. 21.129 Section 21.129... PROCEDURES FOR PRODUCTS AND PARTS Production Under Type Certificate § 21.129 Tests: propellers. Each person manufacturing propellers under a type certificate must give each variable pitch propeller an acceptable...

14 CFR 129.25 - Airplane security.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Airplane security. 129.25 Section 129.25 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIR CARRIERS... AND FOREIGN OPERATORS OF U.S.-REGISTERED AIRCRAFT ENGAGED IN COMMON CARRIAGE General § 129.25 Airplane...

14 CFR 129.25 - Airplane security.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 14 Aeronautics and Space 3 2012-01-01 2012-01-01 false Airplane security. 129.25 Section 129.25 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIR CARRIERS... AND FOREIGN OPERATORS OF U.S.-REGISTERED AIRCRAFT ENGAGED IN COMMON CARRIAGE General § 129.25 Airplane...

14 CFR 129.25 - Airplane security.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 14 Aeronautics and Space 3 2013-01-01 2013-01-01 false Airplane security. 129.25 Section 129.25 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIR CARRIERS... AND FOREIGN OPERATORS OF U.S.-REGISTERED AIRCRAFT ENGAGED IN COMMON CARRIAGE General § 129.25 Airplane...

14 CFR 129.25 - Airplane security.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 14 Aeronautics and Space 3 2014-01-01 2014-01-01 false Airplane security. 129.25 Section 129.25 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIR CARRIERS... AND FOREIGN OPERATORS OF U.S.-REGISTERED AIRCRAFT ENGAGED IN COMMON CARRIAGE General § 129.25 Airplane...

14 CFR 21.129 - Tests: propellers.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 14 Aeronautics and Space 1 2014-01-01 2014-01-01 false Tests: propellers. 21.129 Section 21.129... PROCEDURES FOR PRODUCTS AND PARTS Production Under Type Certificate § 21.129 Tests: propellers. Each person... functional test to determine if it operates properly throughout the normal range of operation. ...

14 CFR 21.129 - Tests: propellers.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 14 Aeronautics and Space 1 2013-01-01 2013-01-01 false Tests: propellers. 21.129 Section 21.129... PROCEDURES FOR PRODUCTS AND PARTS Production Under Type Certificate § 21.129 Tests: propellers. Each person... functional test to determine if it operates properly throughout the normal range of operation. ...

47 CFR 101.129 - Transmitter location.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 47 Telecommunication 5 2010-10-01 2010-10-01 false Transmitter location. 101.129 Section 101.129... SERVICES Technical Standards § 101.129 Transmitter location. (a) The applicant must determine, prior to... adequate to render the service proposed. In cases of questionable antenna locations, it is desirable to...

46 CFR 129.390 - Shore power.

Code of Federal Regulations, 2013 CFR

2013-10-01

... Power Sources and Distribution Systems § 129.390 Shore power. Each vessel that has an electrical system... the switchboard or main distribution panel for the shore-power connection. (d) The circuit breaker... 46 Shipping 4 2013-10-01 2013-10-01 false Shore power. 129.390 Section 129.390 Shipping COAST...

46 CFR 129.390 - Shore power.

Code of Federal Regulations, 2012 CFR

2012-10-01

... Power Sources and Distribution Systems § 129.390 Shore power. Each vessel that has an electrical system... the switchboard or main distribution panel for the shore-power connection. (d) The circuit breaker... 46 Shipping 4 2012-10-01 2012-10-01 false Shore power. 129.390 Section 129.390 Shipping COAST...

46 CFR 129.390 - Shore power.

Code of Federal Regulations, 2014 CFR

2014-10-01

... Power Sources and Distribution Systems § 129.390 Shore power. Each vessel that has an electrical system... the switchboard or main distribution panel for the shore-power connection. (d) The circuit breaker... 46 Shipping 4 2014-10-01 2014-10-01 false Shore power. 129.390 Section 129.390 Shipping COAST...

46 CFR 129.390 - Shore power.

Code of Federal Regulations, 2011 CFR

2011-10-01

... Power Sources and Distribution Systems § 129.390 Shore power. Each vessel that has an electrical system... the switchboard or main distribution panel for the shore-power connection. (d) The circuit breaker... 46 Shipping 4 2011-10-01 2011-10-01 false Shore power. 129.390 Section 129.390 Shipping COAST...

14 CFR 21.129 - Tests: propellers.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 1 2011-01-01 2011-01-01 false Tests: propellers. 21.129 Section 21.129... PROCEDURES FOR PRODUCTS AND PARTS Production Under Type Certificate Only § 21.129 Tests: propellers. Each person manufacturing propellers under a type certificate only shall give each variable pitch propeller an...

14 CFR 21.129 - Tests: propellers.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 1 2010-01-01 2010-01-01 false Tests: propellers. 21.129 Section 21.129... PROCEDURES FOR PRODUCTS AND PARTS Production Under Type Certificate Only § 21.129 Tests: propellers. Each... acceptable functional test to determine if it operates properly throughout the normal range of operation. ...

46 CFR 129.356 - Battery installations.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 4 2010-10-01 2010-10-01 false Battery installations. 129.356 Section 129.356 Shipping... INSTALLATIONS Power Sources and Distribution Systems § 129.356 Battery installations. (a) Large. Each large battery-installation must be located in a locker, room, or enclosed box dedicated solely to the storage of...

46 CFR 129.356 - Battery installations.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 4 2013-10-01 2013-10-01 false Battery installations. 129.356 Section 129.356 Shipping... INSTALLATIONS Power Sources and Distribution Systems § 129.356 Battery installations. (a) Large. Each large battery-installation must be located in a locker, room, or enclosed box dedicated solely to the storage of...

46 CFR 129.356 - Battery installations.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 4 2011-10-01 2011-10-01 false Battery installations. 129.356 Section 129.356 Shipping... INSTALLATIONS Power Sources and Distribution Systems § 129.356 Battery installations. (a) Large. Each large battery-installation must be located in a locker, room, or enclosed box dedicated solely to the storage of...

46 CFR 129.356 - Battery installations.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 4 2012-10-01 2012-10-01 false Battery installations. 129.356 Section 129.356 Shipping... INSTALLATIONS Power Sources and Distribution Systems § 129.356 Battery installations. (a) Large. Each large battery-installation must be located in a locker, room, or enclosed box dedicated solely to the storage of...

46 CFR 129.356 - Battery installations.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 4 2014-10-01 2014-10-01 false Battery installations. 129.356 Section 129.356 Shipping... INSTALLATIONS Power Sources and Distribution Systems § 129.356 Battery installations. (a) Large. Each large battery-installation must be located in a locker, room, or enclosed box dedicated solely to the storage of...

The complex physiology of Cellvibrio japonicus xylan degradation relies on a single cytoplasmic β-xylosidase for xylo-oligosaccharide utilization

DOE PAGES

Blake, Andrew D.; Beri, Nina R.; Guttman, Hadassa S.; ...

2017-12-21

Lignocellulose degradation by microbes plays a central role in global carbon cycling, human gut metabolism, and renewable energy technologies. While considerable effort has been put into understanding the biochemical aspects of lignocellulose degradation, much less work has been done to understand how these enzymes work in an in vivo context. Here in this paper, we report a systems level study of xylan degradation in the saprophytic bacterium Cellvibrio japonicus. Transcriptome analysis indicated seven genes that encode carbohydrate active enzymes were up-regulated during growth with xylan containing media. In-frame deletion analysis of these genes found that only gly43F is critical formore » utilization of xylo-oligosaccharides, xylan, and arabinoxylan. Heterologous expression of gly43F was sufficient for the utilization of xylo-oligosaccharides in Escherichia coli. Additional analysis found that the xyn11A, xyn11B, abf43L, abf43K, and abf51A gene products were critical for utilization of arabinoxylan. Furthermore, a predicted transporter (CJA_1315) was required for effective utilization of xylan substrates, and we propose this unannotated gene be called xntA (xylan transporter A). Our major findings are 1) C. japonicus employs both secreted and surface associated enzymes for xylan degradation, which differs from the strategy used for cellulose degradation, and 2) a single cytoplasmic β-xylosidase is essential for the utilization of xylo-oligosaccharides.« less

The complex physiology of Cellvibrio japonicus xylan degradation relies on a single cytoplasmic β-xylosidase for xylo-oligosaccharide utilization

DOE Office of Scientific and Technical Information (OSTI.GOV)

Blake, Andrew D.; Beri, Nina R.; Guttman, Hadassa S.

Lignocellulose degradation by microbes plays a central role in global carbon cycling, human gut metabolism, and renewable energy technologies. While considerable effort has been put into understanding the biochemical aspects of lignocellulose degradation, much less work has been done to understand how these enzymes work in an in vivo context. Here in this paper, we report a systems level study of xylan degradation in the saprophytic bacterium Cellvibrio japonicus. Transcriptome analysis indicated seven genes that encode carbohydrate active enzymes were up-regulated during growth with xylan containing media. In-frame deletion analysis of these genes found that only gly43F is critical formore » utilization of xylo-oligosaccharides, xylan, and arabinoxylan. Heterologous expression of gly43F was sufficient for the utilization of xylo-oligosaccharides in Escherichia coli. Additional analysis found that the xyn11A, xyn11B, abf43L, abf43K, and abf51A gene products were critical for utilization of arabinoxylan. Furthermore, a predicted transporter (CJA_1315) was required for effective utilization of xylan substrates, and we propose this unannotated gene be called xntA (xylan transporter A). Our major findings are 1) C. japonicus employs both secreted and surface associated enzymes for xylan degradation, which differs from the strategy used for cellulose degradation, and 2) a single cytoplasmic β-xylosidase is essential for the utilization of xylo-oligosaccharides.« less

Effects of trimethoprim on life history parameters, oxidative stress, and the expression of cytochrome P450 genes in the copepod Tigriopus japonicus.

PubMed

Han, Jeonghoon; Lee, Min-Chul; Kim, Duck-Hyun; Lee, Young Hwan; Park, Jun Chul; Lee, Jae-Seong

2016-09-01

Trimethoprim (TMP) is an antibiotic that has been detected in various environments including marine habitats; however, the toxic effects of TMP are poorly understood in non-target marine organisms. In this study, the effects of TMP on mortality, development, reproduction, intracellular reactive oxygen species (ROS) levels, and transcription levels of antioxidant and xenobiotic detoxification-related enzyme genes were investigated in the copepod Tigriopus japonicus. The TMP half lethal dose at 48 h (LC50-48 h) in nauplius and TMP LC50-96 h in adult T. japonicus copepods was determined as 156 mg/L and 200 mg/L, respectively. In TMP-exposed T. japonicus, delayed developmental time and impaired reproduction were observed as harmful effects on the life history parameters. Increased ROS levels were also shown in response to TMP exposure at the highest concentration (100 mg/L TMP) and the expression of antioxidant- (e.g. GST-kappa, GST-sigma) and xenobiotic detoxification (e.g. CYPs)-related genes were upregulated in a time and/or dose-dependent manner in response to TMP. Particularly, significant upregulation of three CYP genes (Tj-CYP3024A2, Tj-CYP3024A3 and Tj-CYP3027C2) were examined, suggesting that these CYP genes are likely playing an important role in the TMP detoxification metabolism in T. japonicus. In summary, we found that TMP induced oxidative stress via the transcriptional regulation of antioxidant- and xenobiotic detoxification-related genes, leading to changes in life history parameters such as developmental delay and reproduction impairment. Three Tj-CYP genes (Tj-CYP3024A2, Tj-CYP3024A3 and Tj-CYP3027C2) could be useful as potential T. japonicus biomarkers in response to antibiotics. Copyright © 2016 Elsevier Ltd. All rights reserved.

Seasonal biochemical changes in composition of body wall tissues of sea cucumber Apostichopus japonicus

NASA Astrophysics Data System (ADS)

Gao, Fei; Xu, Qiang; Yang, Hongsheng

2011-03-01

Seasonal Variation in proximate, amino acid and fatty acid composition of the body wall of sea cucumber Apostichopus japonicus was evaluated. The proximate composition, except for ash content, changed significantly among seasons ( P<0.05). Alanine, glycine, glutamic acid and asparagic acid were the most abundant amino acids. Total amino acid and essential amino acid Contents both varied clearly with seasons ( P<0.05). 16:0 and 16:ln7 were the primary saturated fatty acid (SFA) and monounsaturated fatty acid (MUFA) respectively for all months. EPA (20:5n-3), AA (20:4n-6) and DHA (22:6n-3) were the major polyunsaturated fatty acids (PUFA). The proportions of SFA and PUFA yielded significant seasonal variations ( P<0.001), but MUFA did not changed significantly. The results indicated that the biochemical compositions of the body wall in A. japonicus were significantly influenced by seasons and that the body wall tissue is an excellent source of protein, MUFA and n-3 PUFA for humans.

Action of trypsin on structural changes of collagen fibres from sea cucumber (Stichopus japonicus).

PubMed

Liu, Zi-Qiang; Tuo, Feng-Yan; Song, Liang; Liu, Yu-Xin; Dong, Xiu-Ping; Li, Dong-Mei; Zhou, Da-Yong; Shahidi, Fereidoon

2018-08-01

Trypsin, a representative serine proteinase, was used to hydrolyse the collagen fibres from sea cucumber (Stichopus japonicus) to highlight the role of serine proteinase in the autolysis of sea cucumber. Partial disaggregation of collagen fibres into collagen fibrils upon trypsin treatment occurred. The trypsin treatment also caused a time-dependent release of water-soluble glycosaminoglycans and proteins. Therefore, the degradation of the proteoglycan bridges between collagen fibrils might account for the disaggregation of collagen fibrils. For trypsin-treated collagen fibres (72 h), the collagen fibrils still kept their structural integrity and showed characteristic D-banding pattern, and the dissolution rate of hydroxyproline was just 0.21%. Meanwhile, Fourier transform infrared analysis showed the collagen within trypsin-treated collagen fibres (72 h) still retaining their triple-helical conformation. These results suggested that serine proteinase participated in the autolysis of S. japonicus body wall by damaging the proteoglycan bridges between collagen fibrils and disintegrating the latter. Copyright © 2018 Elsevier Ltd. All rights reserved.

Dietary supplementation of biofloc influences growth performance, physiological stress, antioxidant status and immune response of juvenile sea cucumber Apostichopus japonicus (Selenka).

PubMed

Chen, Jinghua; Ren, Yichao; Wang, Guodong; Xia, Bin; Li, Yuquan

2018-01-01

Bioflocs are rich in various probiotics and bioactive compounds, which play an important role in improving growth and health status of aquatic organisms. A 60-day experiment was conducted to investigate the effects of dietary supplementation of biofloc on growth performance, digestive enzyme activity, physiological stress, antioxidant status, expression of immune-related genes and disease resistance of sea cucumber Apostichopus japonicus. Juvenile sea cucumbers were fed five experimental diets containing graded levels of biofloc from 0% to 20% (referred as B0, B5, B10, B15 and B20, respectively). The results showed that the sea cucumbers at dietary supplementation levels of 10%-15% biofloc had significantly higher specific growth rate (SGR) compared to control group (diet B0). Digestive enzyme activity increased with the increasing of dietary biofloc level, while no significant difference was found between diets B15 and B20. Dietary supplementation of biofloc also had significant influences on physiological stress parameters except for lactate. There was no significant discrepancy in total coelomocytes counts (TCC) in coelomic fluid of sea cucumber between the treatments. Phagocytosis and respiratory burst of cellular immune at 15% and 20% biofloc levels were significantly higher than those of control group. Significant increases in superoxide dismutase (SOD), total nitric oxide synthase (T-NOS), lysozyme (LSZ), acid phosphatase (ACP) and alkaline phosphatase (AKP) activities of sea cucumber were found at highest dietary supplementation level of 20% biofloc. The expression patterns of immune-related genes (i.e., Hsp90, Hsp70, p105, Rel, NOS and LSZ) in tissues of sea cucumber were analyzed between the experimental diets, and a general trend of up-regulation was observed at higher biofloc levels. Furthermore, dietary 10%-20% biofloc significantly reduced cumulative mortality of sea cucumber after being challenged with Vibrio splendidus. In conclusion, dietary

Anthropogenic 129I in the atmosphere: overview over major sources, transport processes and deposition pattern.

PubMed

Reithmeier, H; Lazarev, V; Rühm, W; Nolte, E

2010-10-01

Wet and, to a lesser extent, dry deposition of atmospheric (129)I are known to represent the dominating processes responsible for (129)I in continental environmental samples that are remote from (129)I sources and not directly influenced by any liquid (129)I release of nuclear installations. Up to now, however, little is known about the major emitters and the related global deposition pattern of (129)I. In this work an overview over major sources of (129)I is given, and hitherto unknown time-dependent releases from these were estimated. Total gaseous (129)I releases from the US and former Soviet reprocessing facilities Hanford, Savannah River, Mayak, Seversk and Zheleznogorsk were found to have been 0.53, 0.27, 1.05, 0.23 and 0.14TBq, respectively. These facilities were thus identified as major airborne (129)I emitters. The global deposition pattern due to the (129)I released, depending on geographic latitude and longitude, and on time was studied using a box model describing the global atmospheric transport and deposition of (129)I. The model predictions are compared to (129)I concentrations measured by means of Accelerator Mass Spectrometry (AMS) in water samples that were collected from various lakes in Asia, Africa, America and New Zealand, and to published values. As a result, both pattern and temporal evolution of (129)I deposition values measured in and calculated for different types of environmental samples are, in general, in good agreement. This supports our estimate on atmospheric (129)I releases and the considered substantial transport and deposition mechanisms in our model calculations. Copyright 2010 Elsevier B.V. All rights reserved.

Screen and effect analysis of immunostimulants for sea cucumber, Apostichopus japonicus

NASA Astrophysics Data System (ADS)

Li, Jiye; Sun, Xiuqin; Zheng, Fengrong; Hao, Linhua

2009-02-01

Immunostimulants may improve disease resistance of aquaculture animals by promoting the nonspecific immunity response of the organisms. Five types of saccharides, including chitosan, yeast polysaccharide, burdock oligosaccharide, seaweed polysaccharide and lentinus edodes polysaccharide, were screened for potential use as immunostimulants by using spectrophotometry. The saccharides were injected into Apostichopus japonicus, a sea cucumber, and the lysozyme and superoxide dismutase (SOD) activities of the coelomic fluid and epidermal slime were monitored in six consecutive days. The results show that the lysozyme activity of the animal’s coelomic fluid was significantly stimulated on day 2, day 4 and day 6 after the injection of the saccharides ( P<0.05). The effects of chitosan and yeast polysaccharide were the most notable. The lysozyme activity of the epidermal slime was significantly increased by chitosana, yeast polysaccharide, seaweed polysaccharide, and burdock oligosaccharide on day 1 and day 2 after the injection ( P<0.05). The SOD activity of the coelomic fluid was significantly promoted by the saccharides on day 2 and day 4 post-injection ( P<0.05), while the SOD activity of the epidermal slime increased on day 2. These findings indicate that chitosan and yeast polysaccharide are the most effective immunostimulants and potential healthy anti-disease feedstuff for A. japonicus.

46 CFR 129.390 - Shore power.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 4 2010-10-01 2010-10-01 false Shore power. 129.390 Section 129.390 Shipping COAST... Power Sources and Distribution Systems § 129.390 Shore power. Each vessel that has an electrical system operating at more than 50 volts and has provisions for receiving shore power must meet the requirements of...

7 CFR 400.129 - Salary offset.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 6 2010-01-01 2010-01-01 false Salary offset. 400.129 Section 400.129 Agriculture... Years § 400.129 Salary offset. (a) Debt collection by salary offset is feasible if: the cost to the Government of collection by salary offset does not exceed the amount of the debt; there are no legal...

Lotus Base: An integrated information portal for the model legume Lotus japonicus

PubMed Central

Mun, Terry; Bachmann, Asger; Gupta, Vikas; Stougaard, Jens; Andersen, Stig U.

2016-01-01

Lotus japonicus is a well-characterized model legume widely used in the study of plant-microbe interactions. However, datasets from various Lotus studies are poorly integrated and lack interoperability. We recognize the need for a comprehensive repository that allows comprehensive and dynamic exploration of Lotus genomic and transcriptomic data. Equally important are user-friendly in-browser tools designed for data visualization and interpretation. Here, we present Lotus Base, which opens to the research community a large, established LORE1 insertion mutant population containing an excess of 120,000 lines, and serves the end-user tightly integrated data from Lotus, such as the reference genome, annotated proteins, and expression profiling data. We report the integration of expression data from the L. japonicus gene expression atlas project, and the development of tools to cluster and export such data, allowing users to construct, visualize, and annotate co-expression gene networks. Lotus Base takes advantage of modern advances in browser technology to deliver powerful data interpretation for biologists. Its modular construction and publicly available application programming interface enable developers to tap into the wealth of integrated Lotus data. Lotus Base is freely accessible at: https://lotus.au.dk. PMID:28008948

46 CFR 129.310 - Power sources.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 4 2010-10-01 2010-10-01 false Power sources. 129.310 Section 129.310 Shipping COAST... Power Sources and Distribution Systems § 129.310 Power sources. (a)(1) Each vessel that relies on electricity to power the following loads must be arranged so that the loads can be energized from at least two...

46 CFR 129.310 - Power sources.

Code of Federal Regulations, 2011 CFR

2011-10-01

... Power Sources and Distribution Systems § 129.310 Power sources. (a)(1) Each vessel that relies on electricity to power the following loads must be arranged so that the loads can be energized from at least two... 46 Shipping 4 2011-10-01 2011-10-01 false Power sources. 129.310 Section 129.310 Shipping COAST...

46 CFR 129.310 - Power sources.

Code of Federal Regulations, 2014 CFR

2014-10-01

... Power Sources and Distribution Systems § 129.310 Power sources. (a)(1) Each vessel that relies on electricity to power the following loads must be arranged so that the loads can be energized from at least two... 46 Shipping 4 2014-10-01 2014-10-01 false Power sources. 129.310 Section 129.310 Shipping COAST...

46 CFR 129.310 - Power sources.

Code of Federal Regulations, 2012 CFR

2012-10-01

... Power Sources and Distribution Systems § 129.310 Power sources. (a)(1) Each vessel that relies on electricity to power the following loads must be arranged so that the loads can be energized from at least two... 46 Shipping 4 2012-10-01 2012-10-01 false Power sources. 129.310 Section 129.310 Shipping COAST...

46 CFR 129.310 - Power sources.

Code of Federal Regulations, 2013 CFR

2013-10-01

... Power Sources and Distribution Systems § 129.310 Power sources. (a)(1) Each vessel that relies on electricity to power the following loads must be arranged so that the loads can be energized from at least two... 46 Shipping 4 2013-10-01 2013-10-01 false Power sources. 129.310 Section 129.310 Shipping COAST...

Maltodextrin and sucrose preferences in sweet-sensitive (C57BL/6J) and subsensitive (129P3/J) mice revisited.

PubMed

Ackroff, Karen; Sclafani, Anthony

2016-10-15

Mice are attracted to the tastes of sugar and maltodextrin solutions. Sugar taste is mediated by the T1R2/T1R3 sweet taste receptor, while maltodextrin taste is dependent upon a different as yet unidentified receptor. In a prior study sweet-sensitive C57BL/6J (B6) mice displayed similar preferences for sucrose and maltodextrin solutions in 24-h saccharide vs. water choice tests that exceeded those of sweet-subsensitive 129P3/J (129) mice. In a subsequent experiment reported here, sucrose and maltodextrin (Polycose) preference and acceptance were compared in the two strains in saccharide vs. saccharide choice tests with isocaloric concentrations (0.5-32%). The 129 mice displayed significantly greater maltodextrin preferences than B6 mice at mid-range concentrations (2-8%), while the mice displayed an opposite preference profile at the highest concentration (32%). As in prior studies, 129 mice consumed less total saccharide than B6 mice at lower concentrations. These findings show that the conclusions reached from tastant vs. water tests may differ from those pitting one tastant against another. The increased sucrose preference and intake of B6 mice, relative to 129 mice, is consistent with their sweet-sensitive phenotype. Copyright © 2016 Elsevier Inc. All rights reserved.

Identification and pathogenicity of Vibrio ponticus affecting cultured Japanese sea bass, Lateolabrax japonicus (Cuvier in Cuvier and Valenciennes).

PubMed

Xie, Z Y; Hu, C Q; Zhang, L P; Chen, C; Ren, C H; Shen, Q

2007-07-01

To rapidly determine the causative agent of mass death in Lateolabrax japonicus in Zhelin Bay of Guangdong Province in China in April 2004. Thirty-six strains, numbered sequentially from RP01 to RP36, were isolated from six diseased fish. All of the strains were identified as being of the same vibrio species according to the results of universal primer PCR combined with DGGE (UPPCR-DGGE). RP30 was one of these strains that was randomly selected and analysed by using a morphological, physiological and biochemical plate, Biolog GN2 Microplate System and API 20E system. Furthermore, RP30' 16S rDNA was sequenced and aligned in Genbank. Its virulence to Lateolabrax japonicus (Cuvier in Cuvier and Valenciennes) was also tested. RP30 is most closely related to four Vibrio ponticus strains (99.3% similarity). LD50s were 2.5 (x103 CFU per fish for intraperitoneal inoculation (IP) and 3.2 (x103 CFU per fish for intramuscular inoculation (IM), respectively. The investigated pathogenic agent of Lateolabrax japonicus (Cuvier in Cuvier and Valenciennes) was V. ponticus. UPPCR-DGGE is very helpful in epidemiologic investigation. Interestingly, this is the first report that V. ponticus infects cultured marine fish. DGGE was likewise first introduced to epidemiologic investigation of fish disease.

Changes of exoskeleton surface roughness and expression of crucial participation genes for chitin formation and digestion in the mud crab (Macrophthalmus japonicus) following the antifouling biocide irgarol.

PubMed

Park, Kiyun; Nikapitiya, Chamilani; Kim, Won-Seok; Kwak, Tae-Soo; Kwak, Ihn-Sil

2016-10-01

Irgarol is a common antifoulant present in coastal sediment. The mud crab Macrophthalmus japonicus is one of the most abundant of the macrobenthos in the costal environment, and its exoskeleton has a protective function against various environmental threats. We evaluated the effects of irgarol toxicity on the exoskeleton of M. japonicus, which is the outer layer facing the environment. We analyzed transcriptional expression of exoskeleton, molting, and proteolysis-related genes in the gill and hepatopancreas of these exposed M. japonicus. In addition, changes in survival and exoskeleton surface characteristics were investigated. In the hepatopancreas, mRNA expression of chitinase 1 (Mj-chi1), chitinase 4 (Mj-chi4), and chitinase 5 (Mj-chi5) increased in M. japonicus exposed to all concentrations of irgarol. Mj-chi1 and Mj-chi4 expressions from 1 to 10μgL(-1) were dose- and time-dependent. Ecdysteroid receptor (Mj-EcR), trypsin (Mj-Tryp), and serine proteinase (Mj-SP) in the hepatopancreas were upregulated in response to different exposure levels of irgarol at day 1, 4, or 7. In contrast, gill Mj-chi5, Mj-Tryp, and Mj-SP exhibited late upregulated responses to 10μgL(-1) irgarol compared to the control at day 7. Mj-chi1 showed early upregulation upon exposure to 10μgL(-1) irgarol and Mj-chi4 showed no changes in transcription in the gill. Gill Mj-EcR presented generally downregulated expression patterns. In addition, decreased survival and change of exoskeleton surface roughness were observed in M. japonicus exposed to the three concentrations of irgarol. These results suggest that exposure to irgarol induces changes in the exoskeleton, molting, and proteolysis metabolism of M. japonicus. Copyright © 2016 Elsevier Inc. All rights reserved.

129 Xe chemical shift in human blood and pulmonary blood oxygenation measurement in humans using hyperpolarized 129 Xe NMR.

PubMed

Norquay, Graham; Leung, General; Stewart, Neil J; Wolber, Jan; Wild, Jim M

2017-04-01

To evaluate the dependency of the 129 Xe-red blood cell (RBC) chemical shift on blood oxygenation, and to use this relation for noninvasive measurement of pulmonary blood oxygenation in vivo with hyperpolarized 129 Xe NMR. Hyperpolarized 129 Xe was equilibrated with blood samples of varying oxygenation in vitro, and NMR was performed at 1.5 T and 3 T. Dynamic in vivo NMR during breath hold apnea was performed at 3 T on two healthy volunteers following inhalation of hyperpolarized 129 Xe. The 129 Xe chemical shift in RBCs was found to increase nonlinearly with blood oxygenation at 1.5 T and 3 T. During breath hold apnea, the 129 Xe chemical shift in RBCs exhibited a periodic time modulation and showed a net decrease in chemical shift of ∼1 ppm over a 35 s breath hold, corresponding to a decrease of 7-10 % in RBC oxygenation. The 129 Xe-RBC signal amplitude showed a modulation with the same frequency as the 129 Xe-RBC chemical shift. The feasibility of using the 129 Xe-RBC chemical shift to measure pulmonary blood oxygenation in vivo has been demonstrated. Correlation between 129 Xe-RBC signal and 129 Xe-RBC chemical shift modulations in the lung warrants further investigation, with the aim to better quantify temporal blood oxygenation changes in the cardiopulmonary vascular circuit. Magn Reson Med 77:1399-1408, 2017. © 2016 The Authors Magnetic Resonance in Medicine published by Wiley Periodicals, Inc. on behalf of International Society for Magnetic Resonance in Medicine. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. © 2016 The Authors Magnetic Resonance in Medicine published by Wiley Periodicals, Inc. on behalf of International Society for Magnetic Resonance in Medicine.

Energy metabolism of spermatozoa of the sand dollar Clypeaster japonicus: the endogenous substrate and ultrastructural correlates.

PubMed

Mita, M; Yasumasu, I; Nakamura, M

1994-07-01

Energy metabolism in spermatozoa of the sand dollar-sea urchin Clypeaster japonicus was examined. The spermatozoa contained triglyceride and cholesterol ester besides several kinds of phospholipids and cholesterol. Glycogen and glucose were present at extremely low levels. Following incubation of spermatozoa in seawater, the triglyceride content decreased rapidly. Other lipids, however, remained at constant levels. High lipase activity was demonstrated in the spermatozoa. Also, [1-14C]oleic acid was oxidized to 14CO2. Ultrastructural study showed that lipid globules were present at the bottom of the midpiece. After incubation in seawater, morphological changes in the lipid globules were observed and vacuoles of various sizes appeared near the lipid globules. Thus, it is concluded that C. japonicus spermatozoa obtain energy through oxidation of fatty acid from triglyceride stored in the lipid globules at the midpieces.

7 CFR 1956.125-1956.129 - [Reserved

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 14 2010-01-01 2009-01-01 true [Reserved] 1956.125-1956.129 Section 1956.125-1956.129...) PROGRAM REGULATIONS (CONTINUED) DEBT SETTLEMENT Debt Settlement-Community and Business Programs §§ 1956.125-1956.129 [Reserved] ...

Biological Studies with Laser-Polarized ^129Xe

NASA Astrophysics Data System (ADS)

Tseng, C. H.; Oteiza, E. R.; Wong, G. A.; Walsworth, R. L.; Albert, M. S.; Nascimben, L.; Peled, S.; Sakai, K.; Jolesz, F. A.

1996-05-01

We have studied several biological systems using laser-polarized ^129Xe. In certain tissues magnetic resonance imaging (MRI) using inhaled laser-polarized noble gases may provide images superior to those from conventional proton MRI. High resolution laser-polarized ^3He images of air spaces in the human lung were recently obtained by the Princeton/Duke group. However, ^3He is not very soluble in tissue. Therefore, we are using laser polarized ^129Xe (tissue-soluble), with the long term goal of biomedical functional imaging. We have investigated multi-echo and multi-excitation magnetic resonance detection schemes to exploit the highly non-thermal ^129Xe magnetization produced by the laser polarization technique. We have inhalated live rats with laser-polarized ^129Xe gas and measured three distinct ^129Xe tissue resonances that last 20 to 40 sec. As a demonstration, we obtained a laser polarized ^129Xe image of the human oral cavity. Currently we are measuring the polarization lifetime of ^129Xe dissolved in human blood, the biological transporting medium. These studies and other recent developments will be reported.

32 CFR 1906.112-1906.129 - [Reserved

Code of Federal Regulations, 2012 CFR

2012-07-01

... 32 National Defense 6 2012-07-01 2012-07-01 false [Reserved] 1906.112-1906.129 Section 1906.112-1906.129 National Defense Other Regulations Relating to National Defense CENTRAL INTELLIGENCE AGENCY... INTELLIGENCE AGENCY §§ 1906.112-1906.129 [Reserved] ...

32 CFR 1906.112-1906.129 - [Reserved

Code of Federal Regulations, 2010 CFR

2010-07-01

... 32 National Defense 6 2010-07-01 2010-07-01 false [Reserved] 1906.112-1906.129 Section 1906.112-1906.129 National Defense Other Regulations Relating to National Defense CENTRAL INTELLIGENCE AGENCY... INTELLIGENCE AGENCY §§ 1906.112-1906.129 [Reserved] ...

32 CFR 1906.112-1906.129 - [Reserved

Code of Federal Regulations, 2011 CFR

2011-07-01

... 32 National Defense 6 2011-07-01 2011-07-01 false [Reserved] 1906.112-1906.129 Section 1906.112-1906.129 National Defense Other Regulations Relating to National Defense CENTRAL INTELLIGENCE AGENCY... INTELLIGENCE AGENCY §§ 1906.112-1906.129 [Reserved] ...

32 CFR 1906.112-1906.129 - [Reserved

Code of Federal Regulations, 2013 CFR

2013-07-01

... 32 National Defense 6 2013-07-01 2013-07-01 false [Reserved] 1906.112-1906.129 Section 1906.112-1906.129 National Defense Other Regulations Relating to National Defense CENTRAL INTELLIGENCE AGENCY... INTELLIGENCE AGENCY §§ 1906.112-1906.129 [Reserved] ...

32 CFR 1906.112-1906.129 - [Reserved

Code of Federal Regulations, 2014 CFR

2014-07-01

... 32 National Defense 6 2014-07-01 2014-07-01 false [Reserved] 1906.112-1906.129 Section 1906.112-1906.129 National Defense Other Regulations Relating to National Defense CENTRAL INTELLIGENCE AGENCY... INTELLIGENCE AGENCY §§ 1906.112-1906.129 [Reserved] ...

Feeding behavior and digestive physiology in sea cucumber Apostichopus japonicus.

PubMed

Sun, Jiamin; Zhang, Libin; Pan, Yang; Lin, Chenggang; Wang, Fang; Kan, Rentao; Yang, Hongsheng

2015-02-01

The feeding behavior and digestive physiology of the sea cucumber, Apostichopus japonicus are not well understood. A better understanding may provide useful information for the development of the aquaculture of this species. In this article the tentacle locomotion, feeding rhythms, ingestion rate (IR), feces production rate (FPR) and digestive enzyme activities were studied in three size groups (small, medium and large) of sea cucumber under a 12h light/12h dark cycle. Frame-by-frame video analysis revealed that all size groups had similar feeding strategies using a grasping motion to pick up sediment particles. The tentacle insertion rates of the large size group were significantly faster than those of the small and medium-sized groups (P<0.05). Feeding activities investigated by charge coupled device cameras with infrared systems indicated that all size groups of sea cucumber were nocturnal and their feeding peaks occurred at 02:00-04:00. The medium and large-sized groups also had a second feeding peak during the day. Both IR and FPR in all groups were significantly higher at night than those during the daytime (P<0.05). Additionally, the peak activities of digestive enzymes were 2-4h earlier than the peak of feeding. Taken together, these results demonstrated that the light/dark cycle was a powerful environment factor that influenced biological rhythms of A. japonicus, which had the ability to optimize the digestive processes for a forthcoming ingestion. Copyright © 2014 Elsevier Inc. All rights reserved.

9 CFR 3.129 - Feeding.

Code of Federal Regulations, 2013 CFR

2013-01-01

... Warmblooded Animals Other Than Dogs, Cats, Rabbits, Hamsters, Guinea Pigs, Nonhuman Primates, and Marine... 9 Animals and Animal Products 1 2013-01-01 2013-01-01 false Feeding. 3.129 Section 3.129 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE ANIMAL...

9 CFR 3.129 - Feeding.

Code of Federal Regulations, 2014 CFR

2014-01-01

... Warmblooded Animals Other Than Dogs, Cats, Rabbits, Hamsters, Guinea Pigs, Nonhuman Primates, and Marine... 9 Animals and Animal Products 1 2014-01-01 2014-01-01 false Feeding. 3.129 Section 3.129 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE ANIMAL...

9 CFR 3.129 - Feeding.

Code of Federal Regulations, 2011 CFR

2011-01-01

... Warmblooded Animals Other Than Dogs, Cats, Rabbits, Hamsters, Guinea Pigs, Nonhuman Primates, and Marine... 9 Animals and Animal Products 1 2011-01-01 2011-01-01 false Feeding. 3.129 Section 3.129 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE ANIMAL...

9 CFR 3.129 - Feeding.

Code of Federal Regulations, 2012 CFR

2012-01-01

... Warmblooded Animals Other Than Dogs, Cats, Rabbits, Hamsters, Guinea Pigs, Nonhuman Primates, and Marine... 9 Animals and Animal Products 1 2012-01-01 2012-01-01 false Feeding. 3.129 Section 3.129 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE ANIMAL...

19 CFR 201.112-201.129 - [Reserved

Code of Federal Regulations, 2010 CFR

2010-04-01

... 19 Customs Duties 3 2010-04-01 2010-04-01 false [Reserved] 201.112-201.129 Section 201.112-201.129 Customs Duties UNITED STATES INTERNATIONAL TRADE COMMISSION GENERAL RULES OF GENERAL APPLICATION.... International Trade Commission §§ 201.112-201.129 [Reserved] ...

19 CFR 201.112-201.129 - [Reserved

Code of Federal Regulations, 2011 CFR

2011-04-01

... 19 Customs Duties 3 2011-04-01 2011-04-01 false [Reserved] 201.112-201.129 Section 201.112-201.129 Customs Duties UNITED STATES INTERNATIONAL TRADE COMMISSION GENERAL RULES OF GENERAL APPLICATION.... International Trade Commission §§ 201.112-201.129 [Reserved] ...

19 CFR 201.112-201.129 - [Reserved

Code of Federal Regulations, 2014 CFR

2014-04-01

... 19 Customs Duties 3 2014-04-01 2014-04-01 false [Reserved] 201.112-201.129 Section 201.112-201.129 Customs Duties UNITED STATES INTERNATIONAL TRADE COMMISSION GENERAL RULES OF GENERAL APPLICATION.... International Trade Commission §§ 201.112-201.129 [Reserved] ...

19 CFR 201.112-201.129 - [Reserved

Code of Federal Regulations, 2012 CFR

2012-04-01

... 19 Customs Duties 3 2012-04-01 2012-04-01 false [Reserved] 201.112-201.129 Section 201.112-201.129 Customs Duties UNITED STATES INTERNATIONAL TRADE COMMISSION GENERAL RULES OF GENERAL APPLICATION.... International Trade Commission §§ 201.112-201.129 [Reserved] ...

19 CFR 201.112-201.129 - [Reserved

Code of Federal Regulations, 2013 CFR

2013-04-01

... 19 Customs Duties 3 2013-04-01 2013-04-01 false [Reserved] 201.112-201.129 Section 201.112-201.129 Customs Duties UNITED STATES INTERNATIONAL TRADE COMMISSION GENERAL RULES OF GENERAL APPLICATION.... International Trade Commission §§ 201.112-201.129 [Reserved] ...

45 CFR 2301.112-2301.129 - [Reserved

Code of Federal Regulations, 2010 CFR

2010-10-01

... 45 Public Welfare 4 2010-10-01 2010-10-01 false [Reserved] 2301.112-2301.129 Section 2301.112-2301.129 Public Welfare Regulations Relating to Public Welfare (Continued) ARCTIC RESEARCH COMMISSION... STATES ARCTIC RESEARCH COMMISSION §§ 2301.112-2301.129 [Reserved] ...

45 CFR 2301.112-2301.129 - [Reserved

Code of Federal Regulations, 2011 CFR

2011-10-01

... 45 Public Welfare 4 2011-10-01 2011-10-01 false [Reserved] 2301.112-2301.129 Section 2301.112-2301.129 Public Welfare Regulations Relating to Public Welfare (Continued) ARCTIC RESEARCH COMMISSION... STATES ARCTIC RESEARCH COMMISSION §§ 2301.112-2301.129 [Reserved] ...

36 CFR 909.112-909.129 - [Reserved

Code of Federal Regulations, 2010 CFR

2010-07-01

... 36 Parks, Forests, and Public Property 3 2010-07-01 2010-07-01 false [Reserved] 909.112-909.129 Section 909.112-909.129 Parks, Forests, and Public Property PENNSYLVANIA AVENUE DEVELOPMENT CORPORATION... PENNSYLVANIA AVENUE DEVELOPMENT CORPORATION §§ 909.112-909.129 [Reserved] ...

46 CFR 129.360 - Semiconductor-rectifier systems.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 4 2014-10-01 2014-10-01 false Semiconductor-rectifier systems. 129.360 Section 129.360 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OFFSHORE SUPPLY VESSELS ELECTRICAL INSTALLATIONS Power Sources and Distribution Systems § 129.360 Semiconductor-rectifier systems. (a) Each...

46 CFR 129.360 - Semiconductor-rectifier systems.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 4 2013-10-01 2013-10-01 false Semiconductor-rectifier systems. 129.360 Section 129.360 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OFFSHORE SUPPLY VESSELS ELECTRICAL INSTALLATIONS Power Sources and Distribution Systems § 129.360 Semiconductor-rectifier systems. (a) Each...

46 CFR 129.360 - Semiconductor-rectifier systems.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 4 2012-10-01 2012-10-01 false Semiconductor-rectifier systems. 129.360 Section 129.360 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OFFSHORE SUPPLY VESSELS ELECTRICAL INSTALLATIONS Power Sources and Distribution Systems § 129.360 Semiconductor-rectifier systems. (a) Each...

About 129Xe ∗ in meteoritic nanodiamonds

NASA Astrophysics Data System (ADS)

Fisenko, A. V.; Semjonova, L. F.

2008-08-01

The analysis of excess 129Xe in meteoritic nanodiamonds and the kinetics of its release during stepwise pyrolysis allow to suggest that (1) in the solar nebula 129I atoms were adsorbed onto nanodiamond grains and (or) chemisorbed by forming covalent bonds with carbon atoms. Most 129I atoms existed in a surface connected state, but a minor amount of them was in nanopores of the grains. At radioactive decay of 129I the formed 129Xe ( 129Xe ∗) was trapped by diamond grains due to nuclear recoil. (2) During thermal metamorphism or aqueous alteration, the surface-sited 129I atoms were basically lost. On the basis of these assumptions and calculated concentrations of 129Xe ∗ in meteoritic nanodiamonds it is shown that the minimum closing time of the I-Xe system for meteorites of different chemical classes and low petrologic types may be about one million years relative to the minimally thermally metamorphized CO3 meteorite ALHA 77307. With increasing metamorphic grade the closing time of the I-Xe system increases and can range up to several ten millions years. This tendency is in agreement with an onion-shell model of structure and cooling history of meteorite parent bodies where the temperature increases in the direction from surface to center of the asteroids.

ROS production in homogenate from the body wall of sea cucumber Stichopus japonicus under UVA irradiation: ESR spin-trapping study.

PubMed

Qi, Hang; Dong, Xiu-fang; Zhao, Ya-ping; Li, Nan; Fu, Hui; Feng, Ding-ding; Liu, Li; Yu, Chen-xu

2016-02-01

Sea cucumber Stichopus japonicus (S. japonicus) shows a strong ability of autolysis, which leads to severe deterioration in sea cucumber quality during processing and storage. In this study, to further characterize the mechanism of sea cucumber autolysis, hydroxyl radical production induced by ultraviolet A (UVA) irradiation was investigated. Homogenate from the body wall of S. japonicas was prepared and subjected to UVA irradiation at room temperature. Electron Spin Resonance (ESR) spectra of the treated samples were subsequently recorded. The results showed that hydroxyl radicals (OH) became more abundant while the time of UVA treatment and the homogenate concentration were increased. Addition of superoxide dismutase (SOD), catalase, EDTA, desferal, NaN3 and D2O to the homogenate samples led to different degrees of inhibition on OH production. Metal cations and pH also showed different effects on OH production. These results indicated that OH was produced in the homogenate with a possible pathway as follows: O2(-) → H2O2 → OH, suggesting that OH might be a critical factor in UVA-induced S. japonicus autolysis. Copyright © 2015 Elsevier Ltd. All rights reserved.

14 CFR 129.15 - Flight crewmember certificates.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Flight crewmember certificates. 129.15 Section 129.15 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION... § 129.15 Flight crewmember certificates. No person may act as a flight crewmember unless he holds a...

49 CFR 27.129 - Decisions and notices.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 1 2010-10-01 2010-10-01 false Decisions and notices. 27.129 Section 27.129 Transportation Office of the Secretary of Transportation NONDISCRIMINATION ON THE BASIS OF DISABILITY IN PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Enforcement § 27.129 Decisions and notices. (a...

46 CFR 129.326 - Dual-voltage generators.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 4 2013-10-01 2013-10-01 false Dual-voltage generators. 129.326 Section 129.326 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OFFSHORE SUPPLY VESSELS ELECTRICAL INSTALLATIONS Power Sources and Distribution Systems § 129.326 Dual-voltage generators. If a dual-voltage...

46 CFR 129.326 - Dual-voltage generators.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 4 2012-10-01 2012-10-01 false Dual-voltage generators. 129.326 Section 129.326 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OFFSHORE SUPPLY VESSELS ELECTRICAL INSTALLATIONS Power Sources and Distribution Systems § 129.326 Dual-voltage generators. If a dual-voltage...

46 CFR 129.326 - Dual-voltage generators.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 4 2014-10-01 2014-10-01 false Dual-voltage generators. 129.326 Section 129.326 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OFFSHORE SUPPLY VESSELS ELECTRICAL INSTALLATIONS Power Sources and Distribution Systems § 129.326 Dual-voltage generators. If a dual-voltage...

46 CFR 129.320 - Generators and motors.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 4 2012-10-01 2012-10-01 false Generators and motors. 129.320 Section 129.320 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OFFSHORE SUPPLY VESSELS ELECTRICAL INSTALLATIONS Power Sources and Distribution Systems § 129.320 Generators and motors. (a) Each generator and...

46 CFR 129.320 - Generators and motors.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 4 2013-10-01 2013-10-01 false Generators and motors. 129.320 Section 129.320 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OFFSHORE SUPPLY VESSELS ELECTRICAL INSTALLATIONS Power Sources and Distribution Systems § 129.320 Generators and motors. (a) Each generator and...

46 CFR 129.320 - Generators and motors.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 4 2014-10-01 2014-10-01 false Generators and motors. 129.320 Section 129.320 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OFFSHORE SUPPLY VESSELS ELECTRICAL INSTALLATIONS Power Sources and Distribution Systems § 129.320 Generators and motors. (a) Each generator and...

46 CFR 129.326 - Dual-voltage generators.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 4 2011-10-01 2011-10-01 false Dual-voltage generators. 129.326 Section 129.326 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OFFSHORE SUPPLY VESSELS ELECTRICAL INSTALLATIONS Power Sources and Distribution Systems § 129.326 Dual-voltage generators. If a dual-voltage...

46 CFR 129.560 - Engine-order telegraphs.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 4 2010-10-01 2010-10-01 false Engine-order telegraphs. 129.560 Section 129.560 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OFFSHORE SUPPLY VESSELS ELECTRICAL INSTALLATIONS Miscellaneous Electrical Systems § 129.560 Engine-order telegraphs. No OSV need carry an engine...

45 CFR 2490.112-2490.129 - [Reserved

Code of Federal Regulations, 2012 CFR

2012-10-01

... 45 Public Welfare 4 2012-10-01 2012-10-01 false [Reserved] 2490.112-2490.129 Section 2490.112-2490.129 Public Welfare Regulations Relating to Public Welfare (Continued) JAMES MADISON MEMORIAL... CONDUCTED BY THE JAMES MADISON MEMORIAL FELLOWSHIP FOUNDATION §§ 2490.112-2490.129 [Reserved] ...

45 CFR 2490.112-2490.129 - [Reserved

Code of Federal Regulations, 2013 CFR

2013-10-01

... 45 Public Welfare 4 2013-10-01 2013-10-01 false [Reserved] 2490.112-2490.129 Section 2490.112-2490.129 Public Welfare Regulations Relating to Public Welfare (Continued) JAMES MADISON MEMORIAL... CONDUCTED BY THE JAMES MADISON MEMORIAL FELLOWSHIP FOUNDATION §§ 2490.112-2490.129 [Reserved] ...

45 CFR 2490.112-2490.129 - [Reserved

Code of Federal Regulations, 2011 CFR

2011-10-01

... 45 Public Welfare 4 2011-10-01 2011-10-01 false [Reserved] 2490.112-2490.129 Section 2490.112-2490.129 Public Welfare Regulations Relating to Public Welfare (Continued) JAMES MADISON MEMORIAL... CONDUCTED BY THE JAMES MADISON MEMORIAL FELLOWSHIP FOUNDATION §§ 2490.112-2490.129 [Reserved] ...

45 CFR 2490.112-2490.129 - [Reserved

Code of Federal Regulations, 2014 CFR

2014-10-01

... 45 Public Welfare 4 2014-10-01 2014-10-01 false [Reserved] 2490.112-2490.129 Section 2490.112-2490.129 Public Welfare Regulations Relating to Public Welfare (Continued) JAMES MADISON MEMORIAL... CONDUCTED BY THE JAMES MADISON MEMORIAL FELLOWSHIP FOUNDATION §§ 2490.112-2490.129 [Reserved] ...

46 CFR 129.560 - Engine-order telegraphs.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 4 2014-10-01 2014-10-01 false Engine-order telegraphs. 129.560 Section 129.560 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OFFSHORE SUPPLY VESSELS ELECTRICAL INSTALLATIONS Miscellaneous Electrical Systems § 129.560 Engine-order telegraphs. No OSV need carry an engine...

46 CFR 129.560 - Engine-order telegraphs.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 4 2011-10-01 2011-10-01 false Engine-order telegraphs. 129.560 Section 129.560 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OFFSHORE SUPPLY VESSELS ELECTRICAL INSTALLATIONS Miscellaneous Electrical Systems § 129.560 Engine-order telegraphs. No OSV need carry an engine...

46 CFR 129.560 - Engine-order telegraphs.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 4 2012-10-01 2012-10-01 false Engine-order telegraphs. 129.560 Section 129.560 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OFFSHORE SUPPLY VESSELS ELECTRICAL INSTALLATIONS Miscellaneous Electrical Systems § 129.560 Engine-order telegraphs. No OSV need carry an engine...

45 CFR 2490.112-2490.129 - [Reserved

Code of Federal Regulations, 2010 CFR

2010-10-01

... 45 Public Welfare 4 2010-10-01 2010-10-01 false [Reserved] 2490.112-2490.129 Section 2490.112-2490.129 Public Welfare Regulations Relating to Public Welfare (Continued) JAMES MADISON MEMORIAL... CONDUCTED BY THE JAMES MADISON MEMORIAL FELLOWSHIP FOUNDATION §§ 2490.112-2490.129 [Reserved] ...

Mechanical stress induces neuroendocrine and immune responses of sea cucumber ( Apostichopus japonicus)

NASA Astrophysics Data System (ADS)

Tan, Jie; Li, Fenghui; Sun, Huiling; Gao, Fei; Yan, Jingping; Gai, Chunlei; Chen, Aihua; Wang, Qingyin

2015-04-01

Grading procedure in routine sea cucumber hatchery production is thought to affect juvenile sea cucumber immunological response. The present study investigated the impact of a 3-min mechanical perturbation mimicking the grading procedure on neuroendocrine and immune parameters of the sea cucumber Apostichopus japonicus. During the application of stress, concentrations of noradrenaline and dopamine in coelomic fluid increased significantly, indicating that the mechanical perturbation resulted in a transient state of stress in sea cucumbers. Coelomocytes concentration in coelomic fluid increased transiently after the beginning of stressing, and reached the maximum in 1 h. Whereas, coelomocytes phagocytosis at 3 min, superoxide anion production from 3 min to 0.5 h, acid phosphatase activity at 0.5 h, and phenoloxidase activity from 3 min to 0.5 h were all significantly down-regulated. All of the immune parameters recovered to baseline levels after the experiment was conducted for 8 h, and an immunostimulation occurred after the stress considering the phagocytosis and acid phosphatase activity. The results suggested that, as in other marine invertebrates, neuroendocrine/immune connections exist in sea cucumber A. japonicus. Mechanical stress can elicit a profound influence on sea cucumber neuroendocrine system. Neuroendocrine messengers act in turn to modulate the immunity functions. Therefore, these effects should be considered for developing better husbandry procedures.

46 CFR 129.340 - Cable and wiring.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 4 2010-10-01 2010-10-01 false Cable and wiring. 129.340 Section 129.340 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OFFSHORE SUPPLY VESSELS ELECTRICAL INSTALLATIONS Power Sources and Distribution Systems § 129.340 Cable and wiring. (a) If individual wires, rather than...

Level and source of 129I of environmental samples in Xi'an region, China.

PubMed

Zhang, Luyuan; Zhou, Weijian; Hou, Xiaolin; Chen, Ning; Liu, Qi; He, Chaohui; Fan, Yukun; Luo, Maoyi; Wang, Zhiwen; Fu, Yunchong

2011-09-01

Iodine-129 is widely used as a tracer in various environmental practices such as monitoring of nuclear environmental safety, seawater exchange and transport, geochemical cycle of stable iodine and dating of geological events. The spatial distribution of (129)I concentration varies significantly on global scale because of anthropogenic input from nuclear activities coupled with scarcity of data on environmental (129)I variability in many parts of the world including Asia. Here we report new data on (129)I and (127)I concentrations in soil, vegetation, river water and precipitation collected from Xi'an area, China. The results indicate values for environmental (129)I/(127)I ratios in the investigated area range from 1.1×10(-10) to 43.5×10(-10) with a mean of 20.6×10(-10), which is 1-3 orders of magnitude lower than the ratios observed in Europe, but comparable with those observed in the locations far from direct effect of point release sources and at similar latitude. The main source of (129)I in the investigated area is attributed to the global fallout of both atmospheric nuclear weapons testing and long distance dispersion of fuel reprocessing releases. Copyright © 2011 Elsevier B.V. All rights reserved.

Taste-Evoked Responses to Sweeteners in the Nucleus of the Solitary Tract Differ between C57BL/6ByJ and 129P3/J Mice

PubMed Central

McCaughey, Stuart A.

2008-01-01

C57BL/6ByJ (B6) and 129P3/J (129) mice have different alleles of Tas1r3, which is thought to influence gustatory transduction of sweeteners, but studies have provided conflicting results regarding differences in sweetness perception between these strains. Single-unit taste-evoked activity was measured in the nucleus of the solitary tract (NST) in anesthetized B6 and 129 mice to address this controversy and to provide the first electrophysiological characterization of this nucleus in mice. Neurons had properties similar to those of NST cells in other species, including mean breadth-of-tuning of 0.8 ± 0.0. There were no strain differences in neural responses at 600 or 900 ms after onset, but, with a 5 s evoked period, responses to the sweeteners sucrose, maltose, acesulfame-K, SC-45647, and D-phenylalanine were significantly larger in B6 relative to 129 mice. The strains did not differ in their mean response to NaSaccharin, but it evoked an across-neuron pattern of activity that was more similar to that of sucrose and less similar to that of NaCl in B6 mice compared with 129 mice. Neurons were classified as sucrose, NaCl, or HCl responsive, with the former more common in B6 than 129 mice. Relative to other neurons, sucrose-responsive cells had delayed but more sustained sweetener responses in both strains. The results suggest that B6 mice perceive some sweeteners as more intense, but NaSaccharin as sweeter and less salty, relative to 129 mice. Furthermore, activity evoked by sweeteners includes a phasic response sent to different NST cells than a later tonic response, and only the latter differs between B6 and 129 mice. PMID:17202470

Transcriptional changes in epigenetic modifiers associated with gene silencing in the intestine of the sea cucumber, Apostichopus japonicus (Selenka), during aestivation

NASA Astrophysics Data System (ADS)

Wang, Tianming; Yang, Hongsheng; Zhao, Huan; Chen, Muyan; Wang, Bing

2011-11-01

The sea cucumber, Apostichopus japonicus, undergoes aestivation to improve survival during periods of high-temperature. During aestivation, the metabolic rate is depressed to reduce the consumption of reserved energy. We evaluated the role of epigenetic modification on global gene silencing during metabolic rate depression in the sea cucumber. We compared the expression of epigenetic modifiers in active and aestivating sea cucumbers. The expression of three genes involved in DNA methylation and chromatin remodeling (DNA (cytosine-5)-methyltransferase 1, Methyl-CpG-binding domain protein 2), and Chromodomain-helicase-DNA-binding protein 5) was significantly higher during aestivation (Days 20 and 40). Similarly, we observed an increase in the expression of genes involved in histone acetylation (Histone deacetylase 3) and Histone-binding protein RBBP4) during the early (Days 5 and 10) and late phases (Days 20 and 40) of aestivation. There was no change in the expression of KAT2B, a histone acetyltransferase. However, the expression of histone methylation associated modifiers (Histone-arginine methyltransferase CARMER and Histone-lysine N-methyltransferase MLL5) was significantly higher after 5 d in the aestivating group. The results suggest that the expression of epigenetic modifiers involved in DNA methylation, chromatin remodeling, histone acetylation, and histone methylation is upregulated during aestivation. We hypothesize that these changes regulate global gene silencing during aestivation in A. japonicus.

50 CFR 550.112-550.129 - [Reserved

Code of Federal Regulations, 2010 CFR

2010-10-01

... 50 Wildlife and Fisheries 7 2010-10-01 2010-10-01 false [Reserved] 550.112-550.129 Section 550.112-550.129 Wildlife and Fisheries MARINE MAMMAL COMMISSION ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY MARINE MAMMAL COMMISSION §§ 550.112-550.129...

Use of Gifu Anaerobic Medium for culturing 32 dominant species of human gut microbes and its evaluation based on short-chain fatty acids fermentation profiles.

PubMed

Gotoh, Aina; Nara, Misaki; Sugiyama, Yuta; Sakanaka, Mikiyasu; Yachi, Hiroyuki; Kitakata, Aya; Nakagawa, Akira; Minami, Hiromichi; Okuda, Shujiro; Katoh, Toshihiko; Katayama, Takane; Kurihara, Shin

2017-10-01

Recently, a "human gut microbial gene catalogue," which ranks the dominance of microbe genus/species in human fecal samples, was published. Most of the bacteria ranked in the catalog are currently publicly available; however, the growth media recommended by the distributors vary among species, hampering physiological comparisons among the bacteria. To address this problem, we evaluated Gifu anaerobic medium (GAM) as a standard medium. Forty-four publicly available species of the top 56 species listed in the "human gut microbial gene catalogue" were cultured in GAM, and out of these, 32 (72%) were successfully cultured. Short-chain fatty acids from the bacterial culture supernatants were then quantified, and bacterial metabolic pathways were predicted based on in silico genomic sequence analysis. Our system provides a useful platform for assessing growth properties and analyzing metabolites of dominant human gut bacteria grown in GAM and supplemented with compounds of interest.

49 CFR 28.112-28.129 - [Reserved

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 1 2013-10-01 2013-10-01 false [Reserved] 28.112-28.129 Section 28.112-28.129 Transportation Office of the Secretary of Transportation ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE DEPARTMENT OF TRANSPORTATION §§ 28.112-28.129 [Reserved] ...

49 CFR 28.112-28.129 - [Reserved

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 1 2010-10-01 2010-10-01 false [Reserved] 28.112-28.129 Section 28.112-28.129 Transportation Office of the Secretary of Transportation ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE DEPARTMENT OF TRANSPORTATION §§ 28.112-28.129 [Reserved] ...

Production, characterization and application of monoclonal antibody to spherulocytes: A subpopulation of coelomocytes of Apostichopus japonicus

USDA-ARS?s Scientific Manuscript database

One monoclonal antibody (mAb 3F6) against coelomocytes of sea cucumber Apostichopus japonicus was developed by immunization of Balb/C mice. Analyzed by indirect immunofluorescence assay test (IIFAT), immunocytochemical assay (ICA),Western blotting and fluorescence-activated cell sorter (FACS), mAb 3...

Cell wall polysaccharides released during the alcoholic fermentation by Schizosaccharomyces pombe and S. japonicus: quantification and characterization.

PubMed

Domizio, P; Liu, Y; Bisson, L F; Barile, D

2017-02-01

The present work demonstrates that yeasts belonging to the Schizosaccharomyces genus release a high quantity of polysaccharides of cell wall origin starting from the onset of the alcoholic fermentation. By the end of the alcoholic fermentation, all of the Schizosaccharomyces yeast strains released a quantity of polysaccharides approximately 3-7 times higher than that released by a commercial Saccharomyces cerevisiae yeast strain under the same fermentative conditions of synthetic juice. A higher content of polysaccharide was found in media fermented by Schizosaccharomyces japonicus with respect to that of Schizosaccharomyces pombe. Some of the strains evaluated were also able to produce high levels of pyruvic acid, which has been shown to be an important compound for color stability of wine. The presence of strains with different malic acid consumption patterns along with high polysaccharide release would enable production of naturally modified wines with enhanced mouth feel and reduced acidity. The chemical analysis of the released polysaccharides demonstrated divergence between the two yeast species S. pombe and S. japonicus. A different mannose/galactose ratio and a different percentage of proteins was observed on the polysaccharides released by S. pombe as compared to S. japonicus. Analysis of the proteins released in the media revealed the presence of a glycoprotein with a molecular size around 32-33 kDa only for the species S. japonicus. Mass spectrometry analysis of carbohydrate moieties showed similar proportions among the N-glycan chains released in the media by both yeast species but differences between the two species were also observed. These observations suggest a possible role of rapid MALDI-TOF screening of N-glycans compositional fingerprint as a taxonomic tool for this genus. Polysaccharides release in the media, in particular galactomannoproteins in significant amounts, could make these yeasts particularly interesting also for the industrial

46 CFR 129.326 - Dual-voltage generators.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 4 2010-10-01 2010-10-01 false Dual-voltage generators. 129.326 Section 129.326... INSTALLATIONS Power Sources and Distribution Systems § 129.326 Dual-voltage generators. If a dual-voltage generator is installed on an OSV— (a) The neutral of the dual-voltage system must be solidly grounded at the...

Development of enzyme-linked immunosorbent assay (ELISA) for glutathione S-transferase (GST-S) protein in the intertidal copepod Tigriopus japonicus and its application for environmental monitoring.

PubMed

Rhee, Jae-Sung; Kim, Bo-Mi; Jeong, Chang-Bum; Leung, Kenneth Mei Yee; Park, Gyung Soo; Lee, Jae-Seong

2013-11-01

To utilize the GST-S protein as a useful biomarker for environmental contamination, we developed a polyclonal antibody-based enzyme-linked immunosorbent assay (ELISA) in the intertidal copepod Tigriopus japonicus. Two polyclonal antibodies, TJ-GST-S1 and TJ-GST-S2, were raised against two TJ-GST-S synthetic peptides. Also a recombinant TJ-GST-S protein was purified as a standard for ELISA development. Each polyclonal antibody was tested by Western blot analysis and indirect ELISA. Of two polyclonal antibodies, TJ-GST-S2 ELISA was further employed due to its wide range of detection and the limit of specificity compared to those of TJ-GST-S1 ELISA system. After exposure to 4 metals (Ag, As, Cd, and Cu) to T. japonicus, the amount of TJ-GST-S protein was significantly elevated in a concentration-dependent manner. Also, TJ-GST-S protein was upregulated at relative high concentrations of B[α]P, PCB, and TBT. In this paper, we suggest that T. japonicas ELISA for TJ-GST-S2 is useful as a potential indicator system for marine contaminants. Copyright © 2013 Elsevier Ltd. All rights reserved.

46 CFR 45.129 - Hull fittings: General.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 2 2010-10-01 2010-10-01 false Hull fittings: General. 45.129 Section 45.129 Shipping... Assignment § 45.129 Hull fittings: General. Hull fittings must be securely mounted in the hull so as to avoid increases in hull stresses and must be protected from local damage caused by movement of equipment or cargo. ...

29 CFR 784.129 - “Marine products”.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 29 Labor 3 2013-07-01 2013-07-01 false âMarine productsâ. 784.129 Section 784.129 Labor... Aquatic Products First Processing, Canning, Or Packing of Marine Products Under Section 13(a)(5) § 784.129 “Marine products”. The marine products which form the basis of the exemption are the “fish, shellfish...

29 CFR 784.129 - “Marine products”.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 29 Labor 3 2012-07-01 2012-07-01 false âMarine productsâ. 784.129 Section 784.129 Labor... Aquatic Products First Processing, Canning, Or Packing of Marine Products Under Section 13(a)(5) § 784.129 “Marine products”. The marine products which form the basis of the exemption are the “fish, shellfish...

29 CFR 784.129 - “Marine products”.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 29 Labor 3 2011-07-01 2011-07-01 false âMarine productsâ. 784.129 Section 784.129 Labor... Aquatic Products First Processing, Canning, Or Packing of Marine Products Under Section 13(a)(5) § 784.129 “Marine products”. The marine products which form the basis of the exemption are the “fish, shellfish...

29 CFR 784.129 - “Marine products”.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 29 Labor 3 2010-07-01 2010-07-01 false âMarine productsâ. 784.129 Section 784.129 Labor... Aquatic Products First Processing, Canning, Or Packing of Marine Products Under Section 13(a)(5) § 784.129 “Marine products”. The marine products which form the basis of the exemption are the “fish, shellfish...

29 CFR 784.129 - “Marine products”.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 29 Labor 3 2014-07-01 2014-07-01 false âMarine productsâ. 784.129 Section 784.129 Labor... Aquatic Products First Processing, Canning, Or Packing of Marine Products Under Section 13(a)(5) § 784.129 “Marine products”. The marine products which form the basis of the exemption are the “fish, shellfish...

Particulate matter and heavy metal deposition on the leaves of Euonymus japonicus during the East Asian monsoon in Beijing, China

PubMed Central

Hong, Xiuling; Sun, Liwei

2017-01-01

Plants can be effectively used as bio-monitors of environmental pollution. However, how the particulate matter (PM) and heavy metal retention ability of plants changes in different areas with human disturbance along with monsoon has not yet been investigated in urban ecosystems. In this study, we measured the amount of PM and heavy metals such as Ni, Cr, Cu, Pb, and Zn accumulated by the leaves of Euonymus japonicus during the East Asian monsoon from different functional units in Beijing, China. A rinse-and-weigh method developed in our laboratory was used to determine the mass of the PM, and electro-thermal atomic absorption spectrometry was used for heavy metal analysis. We found that the types of functional units had little influence, whereas the monsoon had a significant effect on the deposition of PM: northwest areas during the monsoon had the lowest effect (with 0.005, 0.453, 0.643, and 1.569 g/m2 fine, coarse, large, and total PM, respectively), and the southeast areas during the monsoon had the highest effect (0.015, 2.687, 1.941, and 4.228 g/m2 for fine, coarse, large, and total PM, respectively). Notable, we found considerable variations in heavy metal accumulation across the functional units analyzed, that is, the accumulation level was higher in communities than in parks (P < 0.0001 for all heavy metals). Moreover, a positive relationship was found between PM retention and heavy metal accumulation by the leaves of E. japonicus. Taken together, our results suggested that the PM and heavy metal retention ability of E. japonicus was sensitive to human disturbance and monsoon in Beijing. Since E. japonicus is a widely distributed tree and has the ability of to purify the atmosphere, it is an ideal plant for mitigating urban environmental pollution. PMID:28662081

Observation of radioactive iodine ((131)I, (129)I) in cropland soil after the Fukushima nuclear accident.

PubMed

Fujiwara, Hideshi

2016-10-01

During the early stages of the Fukushima nuclear accident, the temporal variations of (131)I deposited on the ground and of (131)I accumulated in cropland soil were monitored at a fixed location in Japan. Moreover, concentrations of long-lived radioactive iodine ((129)I) in atmospheric deposits and soil were measured to examine the feasibility of retrospectively reconstructing (131)I levels from the levels of accident-derived (129)I. The exceptionally high levels of (131)I in deposits and soil were attributed to rainfall-related deposition of radionuclides. In the crop field studied, the losses of deposited (131)I and (129)I due to volatilization were small. The atomic ratio (129)I/(131)I in the topsoil corresponded to the same ratio in deposits. The (131)I concentrations measured in the topsoil were very consistent with the (131)I concentrations reconstructed from the (129)I concentrations in the soil. Copyright © 2016 Elsevier B.V. All rights reserved.

Characterization and identification of enzyme-producing microflora isolated from the gut of sea cucumber Apostichopus japonicus

NASA Astrophysics Data System (ADS)

Li, Fenghui; Gao, Fei; Tan, Jie; Fan, Chaojing; Sun, Huiling; Yan, Jingping; Chen, Siqing; Wang, Xiaojun

2016-01-01

Gut microorganisms play an important role in the digestion of their host animals. The purpose of this research was to isolate and assess the enzyme-producing microbes from the Apostichopus japonicus gut. Thirty-nine strains that can produce at least one of the three digestive enzymes (protease, amylase, and cellulase) were qualitatively screened based on their extracellular enzyme-producing abilities. The enzyme-producing strains clustered into eight groups at the genetic similarity level of 100% by analyzing the restriction patterns of 16S rDNA amplified with Mbo I. Phylogenetic analysis revealed that 37 strains belonged to the genus Bacillus and two were members of the genus Virgibacillus. Enzyme-producing capability results indicate that the main enzyme-producing microflora in the A. japonicus gut was Bacillus, which can produce protease, amylase, and cellulase. Virgibacillus, however, can only produce protease. The high enzyme-producing capability of the isolates suggests that the gut microbiota play an important role in the sea cucumber digestive process.

Plate-like permanent dental laminae of upper jaw dentition in adult gobiid fish, Sicyopterus japonicus.

PubMed

Moriyama, Keita; Watanabe, Shun; Iida, Midori; Sahara, Noriyuki

2010-04-01

Sicyopterus japonicus (Teleostei, Gobiidae) possesses a unique upper jaw dentition different from that known for any other teleosts. In the adults, many (up to 30) replacement teeth, from initiation to attachment, are arranged orderly in a semicircular-like strand within a capsule of connective tissue on the labial side of each premaxillary bone. We have applied histological, ultrastructural, and three-dimensional imaging from serial sections to obtain insights into the distribution and morphological features of the dental lamina in the upper jaw dentition of adult S. japonicus. The adult fish has numerous permanent dental laminae, each of which is an infolding of the oral epithelium at the labial side of the functional tooth and forms a thin plate-like structure with a wavy contour. All replacement teeth of a semicircular-like strand are connected to the plate-like dental lamina by the outer dental epithelium and form a tooth family; neighboring tooth families are completely separated from each other. The new tooth germ directly buds off from the ventro-labial margin of the dental lamina, whereas no distinct free end of the dental lamina is present, even adjacent to this region. Cell proliferation concentrated at the ventro-labial margin of the dental lamina suggests that this region is the site for repeated tooth initiation. During tooth development, the replacement tooth migrates along a semicircular-like strand and eventually erupts through the dental lamina into the oral epithelium at the labial side of the functional tooth. This unique thin plate-like permanent dental lamina and the semicircular-like strand of replacement teeth in the upper jaw dentition of adult S. japonicus probably evolved as a dental adaptation related to the rapid replacement of teeth dictated by the specialized feeding habit of this algae-scraping fish.

Phenotypic and genetic characterization of bacteria isolated from diseased cultured sea cucumber (Apostichopus japonicus) in Northeastern China

USDA-ARS?s Scientific Manuscript database

During the winter–spring from 2004 to 2006 in northeastern China cultured Japanese sea cucumber Apostichopus japonicus suffered from a serious disease. Clinical signs included swollen mouth, skin ulceration and massive mortality. Clinical samples taken during this period were studied. Thirty-one bac...

Expression responses of five cold tolerant related genes to two temperature dropping treatments in sea cucumber Apostichopus japonicus

NASA Astrophysics Data System (ADS)

Li, Chengze; Chang, Yaqing; Pang, Zhenguo; Ding, Jun; Ji, Nanjing

2015-03-01

Environmental conditions, including ambient temperature, play important roles in survival, growth development, and reproduction of the Japanese sea cucumber, Apostichopus japonicus. Low temperatures result in slowed growth and skin ulceration disease. In a previous study, we investigated the effect of low temperature on gene expression profiles in A. japonicus by suppression subtractive hybridization (SSH). Genes encoding Ferritin, Lysozyme, Hsp70, gp96, and AjToll were selected from a subtracted cDNA library of A. japonicus under acute cold stress. The transcriptional expression profiles of these genes were investigated in different tissues (coelomocyte, respiratory tree, intestine, longitudinal muscle) after exposure to acute and mild temperature dropping treatments. The results show that (1) the five cold-tolerance-related genes were found in all four tissues and the highest mRNA levels were observed in coelomocyte and respiratory tree; (2) under the temperature dropping treatments, three types of transcriptional regulation patterns were observed: primary suppression followed by up-regulation at -2°C, suppressed expression throughout the two treatments, and more rarely an initial stimulation followed by suppression; and (3) gene expression suppression was more severe under acute temperature dropping than under mild temperature dropping treatment. The five cold-tolerance-related genes that were distributed mainly in coelomocyte and respiratory tissues were generally down-regulated by low temperature stress but an inverse up-regulation event was found at the extreme temperature (-2°C).

Effects of methoprene on oviposition by Aedes japonicus and Culex spp

USGS Publications Warehouse

Butler, M.; Suom, C.; LeBrun, R.A.; Ginsberg, H.S.; Gettman, A.D.

2006-01-01

The mosquito larvicide methoprene is a juvenile growth hormone mimic that is widely used to control mosquitoes. This chemical disrupts normal mosquito development, drastically inhibiting emergence from the pupal to the adult stage. If the presence of methoprene attracts or deters mosquitoes from ovipositing it could have implications for mosquito control. This study evaluates whether methoprene attracts or deters mosquitoes likely to oviposit in catch basins. In a field experiment, methoprene formulated as liquid larvicide did not affect oviposition of either Culex spp. or Aedes japonicus in 19 liter plastic buckets.

Epigenetic inactivation of the MIR129-2 in hematological malignancies

PubMed Central

2013-01-01

Background MIR129-2 has been shown to be a tumor suppressor microRNA hypermethylated in epithelial cancers. Patients and methods Epigenetic inactivation of MIR129-2 was studied by methylation-specific PCR (MSP) in 13 cell lines (eight myeloma and five lymphoma), 15 normal controls and 344 primary samples including acute myeloid leukemia (AML), acute lymphoblastic leukemia (ALL), chronic myeloid leukemia (CML), chronic lymphocytic leukemia (CLL), non-Hodgkin’s lymphoma (NHL), multiple myeloma (MM) at diagnosis, MM at relapse/progression, and monoclonal gammopathy of undetermined significance (MGUS). Expression of MIR129 and its target, SOX4, in cell lines was measured before and after hypomethylating treatment and MIR129 overexpression. MIR129 expression was correlated with MIR129-2 methylation status in primary lymphoma samples. Tumor suppressor function of MIR129 was demonstrated by MTT and trypan blue exclusion assay after MIR129 overexpression. Results The sensitivity of the methylated-MSP was one in 103. Different MSP statuses, including complete methylation, partial methylation, and complete unmethylation, were verified by quantitative bisulfite pyrosequencing. All five lymphoma and seven of eight myeloma cell lines showed complete and partial MIR129-2 methylation. In primary samples, MIR129-2 methylation was absent in AML and CML, but detected in 5% ALL, 45.9% CLL, 49.5% MM at diagnosis, and 59.1% NHL. In CLL, MIR129-2 methylation adversely impacted on survival (p=0.004). In MM, MIR129-2 methylation increased from 27.5% MGUS to 49.5% MM at diagnosis and 41.5% at relapse/progression (p=0.023). In NHL, MIR129-2 methylation was associated with MIR124-1 and MIR203 methylation (p<0.001), and lower MIR129 expression (p=0.009). Hypomethylation treatment of JEKO-1, homozygously methylated for MIR129-2, led to MIR129-2 demethylation and MIR129 re-expression, with downregulation of SOX4 mRNA. Moreover, MIR129 overexpression in both mantle cell lines, JEKO-1 and

Individual variation in growth in sea cucumber Apostichopus japonicus (Selenck) housed individually

NASA Astrophysics Data System (ADS)

Liang, Miao; Dong, Shuanglin; Gao, Qinfeng; Wang, Fang; Tian, Xiangli

2010-09-01

The exceptionally large individual growth variation has been previously recognized in several sea cucumber cohorts. However, there is a lack of information regarding the mechanism of such individual differences. In this study, the sea cucumber Apostichopus japonicus (Selenka) was reared individually in order to eliminate possible effects of social interaction, stocking density, etc. The results showed that there were substantial differences in growth among the sea cucumber individuals during the 100-day experiment. The special growth rate of the sea cucumber individuals differed by up to three folds (from 0.40% to 1.01%), and the coefficient of variation in body weight increased from 12.04% to 40.51%. The final wet body weight, food intake and food conversion efficiency for each sea cucumber were generally positively correlated with their initial wet body weight ( P<0.05). Energy budget of the animals showed that the food energy spent on respiration was much greater (about four folds) but energy deposited for growth was much less for (initially) smaller than for larger A. japonicus. The present result implies that there are obvious genetic differences among the sea cucumber individuals, largely accounting for the individual growth variation of the cohort sea cucumber. These results will provide some basic data for promoting selective breeding and farming of the sea cucumber.

Recent evolution of 129I levels in the Nordic Seas and the North Atlantic Ocean.

PubMed

Vivo-Vilches, Carlos; López-Gutiérrez, José María; Periáñez, Raúl; Marcinko, Charlotte; Le Moigne, Frédéric; McGinnity, Paul; Peruchena, Juan Ignacio; Villa-Alfageme, María

2018-04-15

Most of the anthropogenic radionuclide 129 I released to the marine environment from the nuclear fuel reprocessing plants (NFRP) at Sellafield (England) and La Hague (France) is transported to the Arctic Ocean via the North Atlantic Current and the Norwegian Coastal Current. 129 I concentrations in seawater provides a powerful and well-established radiotracer technique to provide information about the mechanisms which govern water mass transport in the Nordic Seas and the Arctic Ocean and is gaining importance when coupled with other tracers (e.g. CFC, 236 U). In this work, 129 I concentrations in surface and depth profiles from the Nordic Seas and the North Atlantic (NA) Ocean collected from four different cruises between 2011 and 2012 are presented. This work allowed us to i) update information on 129 I concentrations in these areas, required for the accurate use of 129 I as a tracer of water masses; and ii) investigate the formation of deep water currents in the eastern part of the Nordic Seas, by the analysis of 129 I concentrations and temperature-salinity (T-S) diagrams from locations within the Greenland Sea Gyre. In the Nordic Seas, 129 I concentrations in seawater are of the order of 10 9 at·kg -1 , one or two orders of magnitude higher than those measured at the NA Ocean, not so importantly affected by the releases from the NFRP. 129 I concentrations of the order of 10 8 atoms·kg -1 at the Ellet Line and the PAP suggest a direct contribution from the NFRP in the NA Ocean. An increase in the concentrations in the Nordic Seas between 2002 and 2012 has been detected, which agrees with the temporal evolution of the 129 I liquid discharges from the NFRPs in years prior to this. Finally, 129 I profile concentrations, 129 I inventories and T-S diagrams suggest that deep water formation occurred in the easternmost area of the Nordic Seas during 2012. Copyright © 2017 Elsevier B.V. All rights reserved.

Identification and Characterization of MicroRNAs from Longitudinal Muscle and Respiratory Tree in Sea Cucumber (Apostichopus japonicus) Using High-Throughput Sequencing.

PubMed

Wang, Hongdi; Liu, Shikai; Cui, Jun; Li, Chengze; Hu, Yucai; Zhou, Wei; Chang, Yaqing; Qiu, Xuemei; Liu, Zhanjiang; Wang, Xiuli

2015-01-01

MicroRNAs (miRNAs), as a family of non-coding small RNAs, play important roles in the post-transcriptional regulation of gene expression. Sea cucumber (Apostichopus japonicus) is an important economic species which is widely cultured in East Asia. The longitudinal muscle (LTM) and respiratory tree (RPT) are two important tissues in sea cucumber, playing important roles such as respiration and movement. In this study, we identified and characterized miRNAs in the LTM and RPT of sea cucumber (Apostichopus japonicus) using Illumina HiSeq 2000 platform. A total of 314 and 221 conserved miRNAs were identified in LTM and RPT, respectively. In addition, 27 and 34 novel miRNAs were identified in the LTM and RPT, respectively. A set of 58 miRNAs were identified to be differentially expressed between LTM and RPT. Among them, 9 miRNAs (miR-31a-3p, miR-738, miR-1692, let-7a, miR-72a, miR-100b-5p, miR-31b-5p, miR-429-3p, and miR-2008) in RPT and 7 miRNAs (miR-127, miR-340, miR-381, miR-3543, miR-434-5p, miR-136-3p, and miR-300-3p) in LTM were differentially expressed with foldchange value being greater than 10. A total of 14,207 and 12,174 target genes of these miRNAs were predicted, respectively. Functional analysis of these target genes of miRNAs were performed by GO analysis and pathway analysis. This result provided in this work will be useful for understanding biological characteristics of the LTM and RPT of sea cucumber and assisting molecular breeding of sea cucumber for aquaculture.

22 CFR 129.8 - Prior notification.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Prior notification. 129.8 Section 129.8 Foreign Relations DEPARTMENT OF STATE INTERNATIONAL TRAFFIC IN ARMS REGULATIONS REGISTRATION AND LICENSING OF...,000, except for sharing of basic marketing information (e.g., information that does not include...

Thermal experiments with the Asian bush mosquito (Aedes japonicus japonicus) (Diptera: Culicidae) and implications for its distribution in Germany.

PubMed

Reuss, Friederike; Wieser, Andreas; Niamir, Aidin; Bálint, Miklós; Kuch, Ulrich; Pfenninger, Markus; Müller, Ruth

2018-02-05

As ectothermic animals, temperature influences insects in almost every aspect. The potential disease spreading Asian bush mosquito (Aedes japonicus japonicus) is native to temperate East Asia but invasive in several parts of the world. We report on the previously poorly understood temperature-dependence of its life history under laboratory conditions to understand invasion processes and to model temperature niches. To evaluate winter survival, eggs were exposed between 1 day and 14 days to low temperatures (5 °C, 0 °C, -5 °C and -9 °C). Hatching success was drastically decreased after exposure to 0 °C and -5 °C, and the minimal hatching success of 0% was reached at -9 °C after two days. We then exposed larvae to 14 temperatures and assessed their life trait parameters. Larval survival to adulthood was only possible between 10 °C and 31 °C. Based on this, we modelled the optimal (25 °C), minimal (7 °C) and maximal (31 °C) temperature for cumulative female survival. The time to adult emergence ranges from 12 days to 58 days depending on temperature. We used an age-at-emergence-temperature model to calculate the number of potential generations per year for the Asian bush mosquito in Germany with an average of 4.72 potential generations. At lower temperatures, individuals grew larger than at higher temperatures with female R1 length ranging from 3.04 ± 0.1 mm at 31 °C to 4.26 ± 0.2 mm at 15 °C. Reduced egg hatch after exposure to sub-zero temperatures prohibits the establishment of the Asian bush mosquito in large parts of Germany. Larval overwintering is not possible at temperature ≤ 5 °C. The many potential generations displayed per year may contribute to the species' invasion success. This study on the thermal ecology of the Asian bush mosquito adds to our knowledge on the temperature dependence of the species and data could be incorporated in epidemiological and population dynamic modelling.

7 CFR 1221.129 - Right of the Secretary.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 10 2010-01-01 2010-01-01 false Right of the Secretary. 1221.129 Section 1221.129 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING... INFORMATION ORDER Sorghum Promotion, Research, and Information Order Miscellaneous § 1221.129 Right of the...

40 CFR 129.101 - DDT, DDD and DDE.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 22 2011-07-01 2011-07-01 false DDT, DDD and DDE. 129.101 Section 129.101 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) WATER PROGRAMS TOXIC POLLUTANT EFFLUENT STANDARDS Toxic Pollutant Effluent Standards and Prohibitions § 129.101 DDT, DDD and DDE...

7 CFR 1942.129 - Borrower supervision and servicing.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 7 Agriculture 13 2012-01-01 2012-01-01 false Borrower supervision and servicing. 1942.129 Section 1942.129 Agriculture Regulations of the Department of Agriculture (Continued) RURAL HOUSING SERVICE... Facilities Projects § 1942.129 Borrower supervision and servicing. Loans under this subpart are subject to...

7 CFR 1942.129 - Borrower supervision and servicing.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 7 Agriculture 13 2014-01-01 2013-01-01 true Borrower supervision and servicing. 1942.129 Section 1942.129 Agriculture Regulations of the Department of Agriculture (Continued) RURAL HOUSING SERVICE... Facilities Projects § 1942.129 Borrower supervision and servicing. Loans under this subpart are subject to...

7 CFR 1942.129 - Borrower supervision and servicing.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 7 Agriculture 13 2013-01-01 2013-01-01 false Borrower supervision and servicing. 1942.129 Section 1942.129 Agriculture Regulations of the Department of Agriculture (Continued) RURAL HOUSING SERVICE... Facilities Projects § 1942.129 Borrower supervision and servicing. Loans under this subpart are subject to...

7 CFR 1942.129 - Borrower supervision and servicing.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 13 2010-01-01 2009-01-01 true Borrower supervision and servicing. 1942.129 Section 1942.129 Agriculture Regulations of the Department of Agriculture (Continued) RURAL HOUSING SERVICE... Facilities Projects § 1942.129 Borrower supervision and servicing. Loans under this subpart are subject to...

7 CFR 1942.129 - Borrower supervision and servicing.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 7 Agriculture 13 2011-01-01 2009-01-01 true Borrower supervision and servicing. 1942.129 Section 1942.129 Agriculture Regulations of the Department of Agriculture (Continued) RURAL HOUSING SERVICE... Facilities Projects § 1942.129 Borrower supervision and servicing. Loans under this subpart are subject to...

33 CFR 159.129 - Safety: Ignition prevention test.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 33 Navigation and Navigable Waters 2 2011-07-01 2011-07-01 false Safety: Ignition prevention test. 159.129 Section 159.129 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) POLLUTION MARINE SANITATION DEVICES Design, Construction, and Testing § 159.129 Safety: Ignition...

Pre-nuclear level of 129I in Chinese loess-paleosol sections: A search for the natural 129I level for dating in terrestrial environments

NASA Astrophysics Data System (ADS)

Fan, Yukun; Zhou, Weijian; Hou, Xiaolin

2018-06-01

Due to its long half-life (15.7 Myr), radioactive 129I has great potential for dating geologic materials as old as 100 Myr. Thus, knowing the natural level of 129I is crucial to dating applications. The initial ratio of 129I/127I in the ocean has been quantified by a number of researchers who have reached a consensus value. However, the applicability of 129I dating in the terrestrial environment remains problematic because the lack of an initial 129I/127I value. In this work, samples of loess-paleosol sections from the Chinese Loess Plateau (CLP) were analyzed for 129I/127I, aiming to provide an initial 129I/127I ratio that can be adopted for dating purposes in terrestrial environments. A value of (2.0 ± 1.0) × 10-11 for the 129I/127I ratio was found in two investigated loess-paleosol sections from Xifeng and Luochuan, China. This ratio is one order of magnitude higher than the initial value reported for the marine environment. Alteration of the natural initial 129I in the investigated samples due to the downward migration of anthropogenic 129I and by excess fissiogenic 129I from uranium was not supported. Consequently, the 129I/127I ratio measured is considered to be a pristine value, and the difference from that in the marine systems is attributed to an isotopic dilution effect.

A brominated flame retardant 2,2',4,4' tetrabrominated diphenyl ether (BDE-47) leads to lipogenesis in the copepod Tigriopus japonicus.

PubMed

Lee, Min-Chul; Han, Jeonghoon; Lee, Seung-Hwi; Kim, Duck-Hyun; Kang, Hye-Min; Won, Eun-Ji; Hwang, Dae-Sik; Park, Jun Chul; Om, Ae-Son; Lee, Jae-Seong

2016-09-01

De novo lipogenesis (DNL) is a fatty acid synthesis process that requires several genes, including sterol regulatory element binding protein (SREBP), ATP-citrate lyase (ACLY), and acetyl-CoA carboxylase (ACC). DNL up-regulation is able to induce fat accumulation through an increase in fatty acids. To investigate the relationship between DNL up-regulation and the accumulation of fatty acids and lipid droplets in response to 2,2',4,4' tetrabrominated diphenyl ether (BDE-47), we examined DNL in the copepod Tigriopus japonicus. Transcription levels of DNL-related genes were increased after exposure to 2.5μg/L BDE-47 for 24h. After exposure to 2.5μg/L BDE-47, palmitic acid was significantly increased (P<0.05) at days 1 and 4, along with upregulation of fatty acid synthesis-related genes (e.g., desaturases and elongases). However, docosahexaenoic acid and arachidonic acid were down-regulated at days 1 and 4, showing an antagonistic effect. Lipid droplet area significantly increased in Nile red staining analysis after 24h of exposure to 2.5μg/L BDE-47 in T. japonicus, while DNL was down-regulated in response to 500μM salicylate (a lipogenesis inhibitor), indicating that BDE-47 exposure is closely associated with an increase in fatty acids in this copepod. This study provides a better understanding of the effects of BDE-47 on DNL in copepods. Copyright © 2016 Elsevier B.V. All rights reserved.

31 CFR 129.6 - Penalties specified by law.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 31 Money and Finance: Treasury 1 2014-07-01 2014-07-01 false Penalties specified by law. 129.6 Section 129.6 Money and Finance: Treasury Regulations Relating to Money and Finance MONETARY OFFICES, DEPARTMENT OF THE TREASURY PORTFOLIO INVESTMENT SURVEY REPORTING § 129.6 Penalties specified by law...

31 CFR 129.6 - Penalties specified by law.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 31 Money and Finance: Treasury 1 2013-07-01 2013-07-01 false Penalties specified by law. 129.6 Section 129.6 Money and Finance: Treasury Regulations Relating to Money and Finance MONETARY OFFICES, DEPARTMENT OF THE TREASURY PORTFOLIO INVESTMENT SURVEY REPORTING § 129.6 Penalties specified by law...

46 CFR 129.200 - Design, installation, and maintenance.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 4 2014-10-01 2014-10-01 false Design, installation, and maintenance. 129.200 Section 129.200 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OFFSHORE SUPPLY VESSELS ELECTRICAL INSTALLATIONS General Requirements § 129.200 Design, installation, and maintenance. Electrical equipment on a vessel must be designed,...

46 CFR 129.200 - Design, installation, and maintenance.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 4 2012-10-01 2012-10-01 false Design, installation, and maintenance. 129.200 Section 129.200 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OFFSHORE SUPPLY VESSELS ELECTRICAL INSTALLATIONS General Requirements § 129.200 Design, installation, and maintenance. Electrical equipment on a vessel must be designed,...

14 CFR 129.28 - Flightdeck security.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 14 Aeronautics and Space 3 2012-01-01 2012-01-01 false Flightdeck security. 129.28 Section 129.28... Flightdeck security. (a) After August 20, 2002, except for a newly manufactured airplane on a non-revenue...; or the operator must implement a security program approved by the Transportation Security...

14 CFR 129.28 - Flightdeck security.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 14 Aeronautics and Space 3 2014-01-01 2014-01-01 false Flightdeck security. 129.28 Section 129.28... Flightdeck security. (a) After August 20, 2002, except for a newly manufactured airplane on a non-revenue...; or the operator must implement a security program approved by the Transportation Security...

14 CFR 129.28 - Flightdeck security.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Flightdeck security. 129.28 Section 129.28... Flightdeck security. (a) After August 20, 2002, except for a newly manufactured airplane on a non-revenue...; or the operator must implement a security program approved by the Transportation Security...

31 CFR 129.6 - Penalties specified by law.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 31 Money and Finance: Treasury 1 2010-07-01 2010-07-01 false Penalties specified by law. 129.6 Section 129.6 Money and Finance: Treasury Regulations Relating to Money and Finance PORTFOLIO INVESTMENT SURVEY REPORTING § 129.6 Penalties specified by law. Reporters are advised that the Act provides the...

Effects of endogenous cysteine proteinases on structures of collagen fibres from dermis of sea cucumber (Stichopus japonicus).

PubMed

Liu, Yu-Xin; Zhou, Da-Yong; Ma, Dong-Dong; Liu, Zi-Qiang; Liu, Yan-Fei; Song, Liang; Dong, Xiu-Ping; Li, Dong-Mei; Zhu, Bei-Wei; Konno, Kunihiko; Shahidi, Fereidoon

2017-10-01

Autolysis of sea cucumber, caused by endogenous enzymes, leads to postharvest quality deterioration of sea cucumber. However, the effects of endogenous proteinases on structures of collagen fibres, the major biologically relevant substrates in the body wall of sea cucumber, are less clear. Collagen fibres were prepared from the dermis of sea cucumber (Stichopus japonicus), and the structural consequences of degradation of the collagen fibres caused by endogenous cysteine proteinases (ECP) from Stichopus japonicus were examined. Scanning electron microscopic images showed that ECP caused partial disaggregation of collagen fibres into collagen fibrils by disrupting interfibrillar proteoglycan bridges. Differential scanning calorimetry and Fourier transform infrared analysis revealed increased structural disorder of fibrillar collagen caused by ECP. SDS-PAGE and chemical analysis indicated that ECP can liberate glycosaminoglycan, hydroxyproline and collagen fragments from collagen fibres. Thus ECP can cause disintegration of collagen fibres by degrading interfibrillar proteoglycan bridges. Copyright © 2017 Elsevier Ltd. All rights reserved.

Determination of three steroidal saponins from Ophiopogon japonicus (Liliaceae) via high-performance liquid chromatography with mass spectrometry.

PubMed

Wang, Yongyi; Xu, Jinzhong; Qu, Haibin

2013-01-01

A simple and accurate analytical method was developed for simultaneous quantification of three steroidal saponins in the roots of Ophiopogon japonicus via high-performance liquid chromatography (HPLC) with mass spectrometry (MS) in this study. Separation was performed on a Tigerkin C(18) column and detection was performed by mass spectrometry. A mobile phase consisted of 0.02% formic acid in water (v/v) and 0.02% formic acid in acetonitrile (v/v) was used with a flow rate of 0.5 mL min(-1). The quantitative HPLC-MS method was validated for linearity, precision, repeatability, stability, recovery, limits of detection and quantification. This developed method provides good linearity (r >0.9993), intra- and inter-day precisions (RSD <4.18%), repeatability (RSD <5.05%), stability (RSD <2.08%) and recovery (93.82-102.84%) for three steroidal saponins. It could be considered as a suitable quality control method for O. japonicus.

21 CFR 129.37 - Sanitary operations.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Sanitary operations. 129.37 Section 129.37 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) FOOD FOR... shall be protected from contamination. (c) Single-service containers and caps or seals shall be...

7 CFR 1220.129 - State and United States.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 7 Agriculture 10 2012-01-01 2012-01-01 false State and United States. 1220.129 Section 1220.129... CONSUMER INFORMATION Soybean Promotion and Research Order Definitions § 1220.129 State and United States. The terms State and United States include the 50 States of the United States of America, the District...

7 CFR 1220.129 - State and United States.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 7 Agriculture 10 2013-01-01 2013-01-01 false State and United States. 1220.129 Section 1220.129... CONSUMER INFORMATION Soybean Promotion and Research Order Definitions § 1220.129 State and United States. The terms State and United States include the 50 States of the United States of America, the District...

7 CFR 1220.129 - State and United States.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 7 Agriculture 10 2014-01-01 2014-01-01 false State and United States. 1220.129 Section 1220.129... CONSUMER INFORMATION Soybean Promotion and Research Order Definitions § 1220.129 State and United States. The terms State and United States include the 50 States of the United States of America, the District...

7 CFR 1220.129 - State and United States.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 7 Agriculture 10 2011-01-01 2011-01-01 false State and United States. 1220.129 Section 1220.129... CONSUMER INFORMATION Soybean Promotion and Research Order Definitions § 1220.129 State and United States. The terms State and United States include the 50 States of the United States of America, the District...

7 CFR 1220.129 - State and United States.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 10 2010-01-01 2010-01-01 false State and United States. 1220.129 Section 1220.129... CONSUMER INFORMATION Soybean Promotion and Research Order Definitions § 1220.129 State and United States. The terms State and United States include the 50 States of the United States of America, the District...

21 CFR 133.129 - Dry curd cottage cheese.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 21 Food and Drugs 2 2011-04-01 2011-04-01 false Dry curd cottage cheese. 133.129 Section 133.129... FOR HUMAN CONSUMPTION CHEESES AND RELATED CHEESE PRODUCTS Requirements for Specific Standardized Cheese and Related Products § 133.129 Dry curd cottage cheese. (a) Cottage cheese dry curd is the soft...

21 CFR 129.20 - Plant construction and design.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 21 Food and Drugs 2 2013-04-01 2013-04-01 false Plant construction and design. 129.20 Section 129.20 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED... § 129.20 Plant construction and design. (a) The bottling room shall be separated from other plant...

21 CFR 129.20 - Plant construction and design.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 21 Food and Drugs 2 2014-04-01 2014-04-01 false Plant construction and design. 129.20 Section 129.20 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED... § 129.20 Plant construction and design. (a) The bottling room shall be separated from other plant...

21 CFR 129.20 - Plant construction and design.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 21 Food and Drugs 2 2012-04-01 2012-04-01 false Plant construction and design. 129.20 Section 129.20 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED... § 129.20 Plant construction and design. (a) The bottling room shall be separated from other plant...

Improved approach for routine monitoring of 129I activity and 129I/127I atom ratio in environmental samples using TMAH extraction and ICP-MS/MS.

PubMed

Yang, Guosheng; Tazoe, Hirofumi; Yamada, Masatoshi

2018-05-30

To reconstruct 131 I deposition and identify the source of radioiodine due to the Fukushima Daiichi Nuclear Power Plant (FDNPP) accident, 129 I activity and 129 I/ 127 I atom ratio should be obtained by preparing and analyzing large numbers of samples economically. In this study, great efforts were made to realize mild TMAH (tetramethyl ammonium hydroxide) extraction of environmental samples at 90 °C to obtain solutions suitable for the following triple-quadrupole inductively coupled plasma-mass spectrometry (ICP-QQQ) MS/MS mode analysis. After releasing iodine from organic matter in the TMAH extraction solution via K 2 S 2 O 8 oxidation, organic matter was removed effectively by solvent extraction and back-extraction to avoid a serious matrix effect during ICP-QQQ analysis. At the same time, interfering elements, especially, Mo, Cd, and In were also removed effectively, to avoid their undesirable interferences during mass spectrometric analysis. In addition, 0.01% (NH 4 ) 2 SO 3 was selected to introduce I - into ICP-QQQ to ensure there was no memory effect and a stable signal was gotten. Subsequently, ICP-QQQ MS/MS mode was applied to further eliminate polyatomic interferences ( 127 I(H 2 and D) + , 97 MoO 2 + , 113 InO + , and 113 CdO + ) and isobaric interference from 129 Xe + . Finally, the developed method was successfully applied to measure 129 I/ 127 I atom ratios ((2.61-27.0) × 10 -7 ) and 129 I activities (3.51-11.4 mBq kg -1 ) in soil samples. The developed method allows a greater number of ordinary laboratories to participate in the field of radioiodine analysis. Copyright © 2018 Elsevier B.V. All rights reserved.

Regulation of nuclear envelope dynamics via APC/C is necessary for the progression of semi-open mitosis in Schizosaccharomyces japonicus.

PubMed

Aoki, Keita; Shiwa, Yuh; Takada, Hiraku; Yoshikawa, Hirofumi; Niki, Hironori

2013-09-01

Three types of mitosis, which are open, closed or semi-open mitosis, function in eukaryotic cells, respectively. The open mitosis involves breakage of the nuclear envelope before nuclear division, whereas the closed mitosis proceeds with an intact nuclear envelope. To understand the mechanism and significance of three types of mitotic division in eukaryotes, we investigated the process of semi-open mitosis, in which the nuclear envelope is only partially broken, in the fission yeast Schizosaccharomyces japonicus. In anaphase-promoting complex/cyclosome (APC/C) mutants of Sz. japonicus, the nuclear envelope remained relatively intact during anaphase, resulting in impaired semi-open mitosis. As a suppressor of apc2 mutant, a mutation of Oar2, which was a 3-oxoacyl-[acyl carrier protein] reductase, was obtained. The level of the Oar2, which had two destruction-box motifs recognized by APC/C, was increased in APC/C mutants. Furthermore, the defective semi-open mitosis observed in an apc2 mutant was restored by mutated oar2+. Based on these findings, we propose that APC/C regulates the dynamics of the nuclear envelope through degradation of Oar2 dependent on APC/C during the metaphase-to-anaphase transition of semi-open mitosis in Sz. japonicus. © 2013 The Authors Genes to Cells © 2013 by the Molecular Biology Society of Japan and Wiley Publishing Asia Pty Ltd.

Differential expression of acid invertase genes in roots of metallicolous and non-metallicolous populations of Rumex japonicus under copper stress.

PubMed

Huang, Wu-Xing; Cao, Yi; Huang, Li-Juan; Ren, Cong; Xiong, Zhi-Ting

2011-09-01

Recent evidence indicates that during copper (Cu) stress, the roots of metallicolous plants manifest a higher activity of acid invertase enzymes, which are rate-limiting in sucrose catabolism, than non-metallicolous plants. To test whether the higher activity of acid invertases is the result of higher expression of acid invertase genes, we isolated partial cDNAs for acid invertases from two populations of Rumex japonicus (from metalliferous and non-metalliferous soils), determined their nucleotide sequences, and designed primers to measure changes in transcript levels during Cu stress. We also determined the growth of the plants' roots, Cu accumulation, and acid invertase activities. The seedlings of R. japonicus were exposed to control or 20 μM Cu(2+) for 6d under hydroponic conditions. The transcript level and enzyme activity of acid invertases in metallicolous plants were both significantly higher than those in non-metallicolous plants when treated with 20 μM. Under Cu stress, the root length and root biomass of metallicolous plants were also significantly higher than those of non-metallicolous plants. The results suggested that under Cu stress, the expression of acid invertase genes in metallicolous plants of R. japonicus differed from those in non-metallicolous plants. Furthermore, the higher acid invertase activities of metallicolous plants under Cu stress could be due in part to elevated expression of acid invertase genes. Copyright © 2011 Elsevier Ltd. All rights reserved.

Phagocytic activities of hemocytes from the deep-sea symbiotic mussels Bathymodiolus japonicus, B. platifrons, and B. septemdierum.

PubMed

Tame, Akihiro; Yoshida, Takao; Ohishi, Kazue; Maruyama, Tadashi

2015-07-01

Deep-sea mytilid mussels harbor symbiotic bacteria in their gill epithelial cells that are horizontally or environmentally transmitted to the next generation of hosts. To understand the immune defense system in deep-sea symbiotic mussels, we examined the hemocyte populations of the symbiotic Bathymodiolus mussel species Bathymodiolus japonicus, Bathymodiolus platifrons, and Bathymodiolus septemdierum, and characterized three types of hemocytes: agranulocytes (AGs), basophilic granulocytes (BGs), and eosinophilic granulocytes (EGs). Of these, the EG cells were the largest (diameter, 8.4-10.0 μm) and had eosinophilic cytoplasm with numerous eosinophilic granules (diameter, 0.8-1.2 μm). Meanwhile, the BGs were of medium size (diameter, 6.7-8.0 μm) and contained small basophilic granules (diameter, 0.3-0.4 μm) in basophilic cytoplasm, and the AGs, the smallest of the hemocytes (diameter, 4.8-6.0 μm), had basophilic cytoplasm lacking granules. A lectin binding assay revealed that concanavalin A bound to all three hemocyte types, while wheat germ agglutinin bound exclusively to EGs and BGs. The total hemocyte population densities within the hemolymph of all three Bathymodiolus mussel species were similar (8.4-13.3 × 10(5) cells/mL), and the percentages of circulating AGs, BGs, and EGs in the hemolymph of these organisms were 44.7-48.5%, 14.3-17.6%, and 34.3-41.0%, respectively. To analyze the functional differences between these hemocytes, the phagocytic activity and post-phagocytic phagosome-lysosome fusion events were analyzed in each cell type using a fluorescent Alexa Fluor(®) 488-conjugated Escherichia coli bioparticle and a LysoTracker(®) lysosomal marker, respectively. While the AGs exhibited no phagocytic activity, both types of granulocytes were phagocytic. Of the three hemocyte types, the EGs exhibited the highest level of phagocytic activity as well as rapid phagosome-lysosome fusion, which occurred within 2 h of incubation. Meanwhile, the BGs showed

Characterization and application of monoclonal antibodies against Shewanella marisflavi, a novel pathogen of Apostichopus japonicus

USDA-ARS?s Scientific Manuscript database

Shewanella marisflavi strain AP629 was certified as a novel pathogen of the sea cucumber Apostichopus japonicus. In this study, four monoclonal antibodies (MAbs) (3C1, 3D9, 2F2, 2A8) against strain AP629 were developed by immunizing Balb/C mice. 3C1 and 3D9 recognized S. marisflavi only, showing no ...

Hyperpolarized 3He and 129Xe magnetic resonance imaging apparent diffusion coefficients: physiological relevance in older never‐ and ex‐smokers

PubMed Central

Kirby, Miranda; Ouriadov, Alexei; Svenningsen, Sarah; Owrangi, Amir; Wheatley, Andrew; Etemad‐Rezai, Roya; Santyr, Giles E.; McCormack, David G.; Parraga, Grace

2014-01-01

Abstract Noble gas pulmonary magnetic resonance imaging (MRI) is transitioning away from 3He to 129Xe gas, but the physiological/clinical relevance of 129Xe apparent diffusion coefficient (ADC) parenchyma measurements is not well understood. Therefore, our objective was to generate 129Xe MRI ADC for comparison with 3He ADC and with well‐established measurements of alveolar structure and function in older never‐smokers and ex‐smokers with chronic obstructive pulmonary disease (COPD). In four never‐smokers and 10 COPD ex‐smokers, 3He (b = 1.6 sec/cm2) and 129Xe (b = 12, 20, and 30 sec/cm2) ADC, computed tomography (CT) density‐threshold measurements, and the diffusing capacity for carbon monoxide (DLCO) were measured. To understand regional differences, the anterior–posterior (APG) and superior–inferior (∆SI) ADC differences were evaluated. Compared to never‐smokers, COPD ex‐smokers showed greater 3He ADC (P = 0.006), 129Xe ADCb12 (P = 0.006), and ADCb20 (P = 0.006), but not for ADCb30 (P > 0.05). Never‐smokers and COPD ex‐smokers had significantly different APG for 3He ADC (P = 0.02), 129Xe ADCb12 (P = 0.006), and ADCb20 (P = 0.01), but not for ADCb30 (P > 0.05). ∆SI for never‐ and ex‐smokers was significantly different for 3He ADC (P = 0.046), but not for 129Xe ADC (P > 0.05). There were strong correlations for DLCO with 3He ADC and 129Xe ADCb12 (both r = −0.95, P < 0.05); in a multivariate model 129Xe ADCb12 was the only significant predictor of DLCO (P = 0.049). For COPD ex‐smokers, CT relative area <−950 HU (RA950) correlated with 3He ADC (r = 0.90, P = 0.008) and 129Xe ADCb12 (r = 0.85, P = 0.03). In conclusion, while 129Xe ADCb30 may be appropriate for evaluating subclinical or mild emphysema, in this small group of never‐smokers and ex‐smokers with moderate‐to‐severe emphysema, 129Xe ADCb12 provided a physiologically appropriate estimate of gas exchange abnormalities and alveolar microstructure. PMID:25347853

27 CFR 19.129 - Change in trade name.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 27 Alcohol, Tobacco Products and Firearms 1 2013-04-01 2013-04-01 false Change in trade name. 19.129 Section 19.129 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU... Amending An Operating Permit § 19.129 Change in trade name. If the proprietor intends to change or add a...

27 CFR 19.129 - Change in trade name.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 27 Alcohol, Tobacco Products and Firearms 1 2011-04-01 2011-04-01 false Change in trade name. 19.129 Section 19.129 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU... Amending An Operating Permit § 19.129 Change in trade name. If the proprietor intends to change or add a...

Production of the Functional Trisaccharide 1-Kestose from Cane Sugar Molasses Using Aspergillus japonicus β-Fructofuranosidase.

PubMed

Hirabayashi, Katsuki; Kondo, Nobuhiro; Toyota, Hiroshi; Hayashi, Sachio

2017-01-01

We report the production of the functional trisaccharide 1-kestose, O-β-D-fructofuranosyl-(2→1)-β-D-fructofuranosyl α-D-glucopyranoside, by β-fructofuranosidase from Aspergillus japonicus using sugar cane molasses as substrate. Sucrose in cane sugar molasses acted as a fructosyl donor and acceptor for the enzyme. The tetrasaccharide nystose, O-β-D-fructofuranosyl-(2→1)-β-D-fructofuranosyl-(2→1)-β-D-fructofuranosyl α-D-glucopyranoside, was produced from 1-kestose. Cane sugar molasses mixed with water provided a better substrate solution for β-fructofuranosidase compared to undiluted molasses due to the high concentration of product inhibitors such as glucose and fructose in molasses. The maximum concentration of 1-kestose obtained was 84.9 mg/ml and the maximum production efficiency was 32.3% after 24 h reaction at 40 °C. The maximum efficiency of combined fructo-oligosaccharide (1-kestose and nystose) production was 40.6%. 1-Kestose was therefore produced via a fructosyl-transfer reaction catalyzed by β-fructofuranosidase from A. japonicus.

27 CFR 19.129 - Change in trade name.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 27 Alcohol, Tobacco Products and Firearms 1 2012-04-01 2012-04-01 false Change in trade name. 19.129 Section 19.129 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY LIQUORS DISTILLED SPIRITS PLANTS Changes to Registrations and Permits Rules for Amending An Operating Permit § 19.129 Chang...

27 CFR 19.129 - Change in trade name.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 27 Alcohol, Tobacco Products and Firearms 1 2014-04-01 2014-04-01 false Change in trade name. 19.129 Section 19.129 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY ALCOHOL DISTILLED SPIRITS PLANTS Changes to Registrations and Permits Rules for Amending An Operating Permit § 19.129 Chang...

Effects of triclosan (TCS) on fecundity, the antioxidant system, and oxidative stress-mediated gene expression in the copepod Tigriopus japonicus.

PubMed

Park, Jun Chul; Han, Jeonghoon; Lee, Min-Chul; Seo, Jung Soo; Lee, Jae-Seong

2017-08-01

Triclosan (TCS) is an antimicrobial agent that has been widely dispersed and detected in the marine environment. However, the effects of TCS in marine invertebrates are poorly understood. In this study, the effects of TCS on life cycle history (e.g. mortality and fecundity) along with cellular reactive oxygen species (ROS) levels, GSH content, antioxidant enzymatic activities, and mRNA expression levels of oxidative stress-mediated genes were measured in the copepod Tigriopus japonicus. The no observed effect concentration (NOEC) and median lethal concentration (LC50) of TCS in the adult stage were determined to be 300μg/L and 437.476μg/L, respectively, while in the nauplius stages the corresponding values were 20μg/L, and 51.76μg/L, respectively. Fecundity was significantly reduced (P<0.05) in response to TCS at 100μg/L. Concentration- and time-dependent analysis of ROS, GSH content (%), and antioxidant enzymatic activities (e.g. GST, GPx, and SOD) were significantly increased (P<0.05) in response to TCS exposure. Additionally, mRNA expression of detoxification (e.g., CYPs) and antioxidant (e.g., glutathione S-transferase-sigma isoforms, Cu/Zn superoxide dismutase, catalase) genes was modulated in response to TCS exposure at different concentrations over a 24h period. Our results revealed that TCS can induce reduced fecundity and oxidative stress with transcriptional regulation of oxidative stress-mediated genes with activation of the antioxidant system in the copepod T. japonicus. Copyright © 2017 Elsevier B.V. All rights reserved.

44 CFR 12.9 - Closed meetings.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 44 Emergency Management and Assistance 1 2010-10-01 2010-10-01 false Closed meetings. 12.9 Section... SECURITY GENERAL ADVISORY COMMITTEES § 12.9 Closed meetings. (a) The requirements of § 12.8 (c) and (d) of this part that meetings shall be open to the public and that the public shall be afforded an...

NMR of laser-polarized 129Xe in blood foam

NASA Technical Reports Server (NTRS)

Tseng, C. H.; Peled, S.; Nascimben, L.; Oteiza, E.; Walsworth, R. L.; Jolesz, F. A.

1997-01-01

Laser-polarized 129Xe dissolved in a foam preparation of fresh human blood was investigated. The NMR signal of 129Xe dissolved in blood was enhanced by creating a foam in which the dissolved 129Xe exchanged with a large reservoir of gaseous laser-polarized 129Xe. The dissolved 129Xe T1 in this system was found to be significantly shorter in oxygenated blood than in deoxygenated blood. The T1 of 129Xe dissolved in oxygenated blood foam was found to be approximately 21 (+/-5) s, and in deoxygenated blood foam to be greater than 40 s. To understand the oxygenation trend, T1 measurements were also made on plasma and hemoglobin foam preparations. The measurement technique using a foam gas-liquid exchange interface may also be useful for studying foam coarsening and other liquid physical properties.

STS-129 Crew Members on the Middeck

NASA Image and Video Library

2009-11-20

S129-E-007132 (20 Nov. 2009) --- Astronauts Charles O. Hobaugh (left), STS-129 commander; and Robert L. Satcher Jr., mission specialist, are pictured on the middeck of Space Shuttle Atlantis while docked with the International Space Station.

Acute oral toxicity of the ethyl acetate fraction of Orostachys japonicus in mice.

PubMed

Kim, Seon-Hee; Ryu, Deok-Seon; Lee, Hyeong-Seon; Shin, Hye-Ryoung; Kwon, Ji-Hye; Lee, Dong-Seok

2014-10-01

Orostachys japonicus (Crassulaceae) is referred to as Wa-song in Korea. It is used as an anti-inflammatory, antifebrile, hemostatic, and anti cancer agent, and as an antidote. The purpose of this study was to evaluate the acute toxicity of the ethyl acetate fraction of O. japonicus (OJE) after the oral administration in Balb/c mice of both sexes. Mice were oral administered a single doses of 500, 1000, and 2000 mg/kg of body weight and were monitored for 14 d. Biochemical parameters [aspartate amino transferase (AST), alanine amino transferase (ALT), alkaline phosphatase (ALP), total protein (TP), globulin (GB), total cholesterol (TC), triglyceride (TG), blood urea nitrogen (BUN), and creatinine (CR)] and histopathological examination of liver were performed. No animals died and no toxic changes were observed in clinical signs, body weight, and organ weight. The LD50 of orally administered OJE was higher than 2000 mg/kg/d in both sexes. No toxicological findings were found in biochemical parameters. In histophathological examination, neutrophilic infiltration was observed at a dose of 2000 mg/kg group in both sexes. These finding suggest that oral administration of OJE does not produce acute toxicity. Therefore, these results could provide satisfactory preclinical evidence of safety to launch clinical trials on standardized formulation of OJE to be a biohealth product.

Space Shuttle Atlantis Landing / STS-129 Mission

NASA Image and Video Library

2009-11-27

PHOTO CREDIT: NASA or National Aeronautics and Space Administration CAPE CANAVERAL, Fla. - Space shuttle Atlantis touches down on Runway 33 at the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida after 11 days in space, completing the 4.5-million mile STS-129 mission on orbit 171. Main gear touchdown was at 9:44:23 a.m. EDT. Nose gear touchdown was at 9:44:36 a.m., and wheels stop was at 9:45:05 a.m. Aboard Atlantis are Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr.; and Expedition 20 and 21 Flight Engineer Nicole Stott who spent 87 days aboard the International Space Station. STS-129 is the final space shuttle Expedition crew rotation flight on the manifest. On STS-129, the crew delivered 14 tons of cargo to the orbiting laboratory, including two ExPRESS Logistics Carriers containing spare parts to sustain station operations after the shuttles are retired next year. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Jim Grossmann

Identification and characterization of 43 microsatellite markers derived from expressed sequence tags of the sea cucumber ( Apostichopus japonicus)

NASA Astrophysics Data System (ADS)

Jiang, Qun; Li, Qi; Yu, Hong; Kong, Lingfeng

2011-06-01

The sea cucumber Apostichopus japonicus is a commercially and ecologically important species in China. A total of 3056 potential unigenes were generated after assembling 7597 A. japonicus expressed sequence tags (ESTs) downloaded from Gen-Bank. Two hundred and fifty microsatellite-containing ESTs (8.18%) and 299 simple sequence repeats (SSRs) were detected. The average density of SSRs was 1 per 7.403 kb of EST after redundancy elimination. Di-nucleotide repeat motifs appeared to be the most abundant type with a percentage of 69.90%. Of the 126 primer pairs designed, 90 amplified the expected products and 43 showed polymorphism in 30 individuals tested. The number of alleles per locus ranged from 2 to 26 with an average of 7.0 alleles, and the observed and expected heterozygosities varied from 0.067 to 1.000 and from 0.066 to 0.959, respectively. These new EST-derived microsatellite markers would provide sufficient polymorphism for population genetic studies and genome mapping of this sea cucumber species.

Phoretic mites and their hyperphoretic fungi associated with flying Ips typographus japonicus Niijima (Col., Scolytidae) in Japan

Treesearch

J.C. Moser; T.J. Perry; K. Furuta

1997-01-01

Flying Ips typographus japonicus from Hokkaido (Japan) carried 12 species of phoretic mites, three of which were not previously recorded in Europe. The mite biologies were diverse, including specialists feeding on microorganisms, beetle eggs, and nematodes which were common under beetle elytra. Hyperphoretic on these mites were seven distinct species of fungal spores...

WAFs lead molting retardation of naupliar stages with down-regulated expression profiles of chitin metabolic pathway and related genes in the copepod Tigriopus japonicus.

PubMed

Hwang, Dae-Sik; Lee, Min-Chul; Kyung, Do-Hyun; Kim, Hui-Su; Han, Jeonghoon; Kim, Il-Chan; Puthumana, Jayesh; Lee, Jae-Seong

2017-03-01

Oil pollution is considered being disastrous to marine organisms and ecosystems. As molting is critical in the developmental process of arthropods in general and copepods, in particular, the impact will be adverse if the target of spilled oil is on molting. Thus, we investigated the harmful effects of water accommodated fractions (WAFs) of crude oil with an emphasis on inhibition of chitin metabolic pathways related genes and developmental retardation in the copepod Tigriopus japonicus. Also, we analysed the ontology and domain of chitin metabolic pathway genes and mRNA expression patterns of developmental stage-specific genes. Further, the developmental retardation followed by transcriptional modulations in nuclear receptor genes (NR) and chitin metabolic pathway-related genes were observed in the WAFs-exposed T. japonicus. As a result, the developmental time was found significantly (P<0.05) delayed in response to 40% WAFs in comparison with that of control. Moreover, the NR gene, HR3 and chitinases (CHT9 and CHT10) were up-regulated in N4-5 stages, while chitin synthase genes (CHS-1, CHS-2-1, and CHS-2-2) down-regulated in response to WAFs. In brief, a high concentration of WAFs repressed nuclear receptor genes but elicited activation of some of the transcription factors at low concentration of WAFs, resulting in suppression of chitin synthesis. Thus, we suggest that WAF can lead molting retardation of naupliar stages in T. japonicus through down-regulations of chitin metabolism. These findings will provide a better understanding of the mode of action of chitin biosynthesis associated with molting mechanism in WAF-exposed T. japonicus. Copyright © 2016 Elsevier Inc. All rights reserved.

DNA methylation levels analysis in four tissues of sea cucumber Apostichopus japonicus based on fluorescence-labeled methylation-sensitive amplified polymorphism (F-MSAP) during aestivation.

PubMed

Zhao, Ye; Chen, Muyan; Storey, Kenneth B; Sun, Lina; Yang, Hongsheng

2015-03-01

DNA methylation plays an important role in regulating transcriptional change in response to environmental stimuli. In the present study, DNA methylation levels of tissues of the sea cucumber Apostichopus japonicus were analyzed by the fluorescence-labeled methylation-sensitive amplified polymorphism (F-MSAP) technique over three stages of the aestivation cycle. Overall, a total of 26,963 fragments were amplified including 9112 methylated fragments among four sea cucumber tissues using 18 pairs of selective primers. Results indicated an average DNA methylation level of 33.79% for A. japonicus. The incidence of DNA methylation was different across tissue types in the non-aestivation stage: intestine (30.16%), respiratory tree (27.61%), muscle (27.94%) and body wall (56.25%). Our results show that hypermethylation accompanied deep-aestivation in A. japonicus, which suggests that DNA methylation may have an important role in regulating global transcriptional suppression during aestivation. Further analysis indicated that the main DNA modification sites were focused on intestine and respiratory tree tissues and that full-methylation but not hemi-methylation levels exhibited significant increases in the deep-aestivation stage. Copyright © 2014 Elsevier Inc. All rights reserved.

Effect of potential probiotic Rhodotorula benthica D30 on the growth performance, digestive enzyme activity and immunity in juvenile sea cucumber Apostichopus japonicus.

PubMed

Wang, Ji-hui; Zhao, Liu-qun; Liu, Jin-feng; Wang, Han; Xiao, Shan

2015-04-01

The effects of dietary addition of yeast Rhodotorula benthica (R. benthica) D30 which isolated from local sea mud at levels of 0 (control), 10(5), 10(6) and 10(7) CFU/g feed on the growth performance, digestive enzyme activity, immunity and disease resistance of juvenile sea cucumber Apostichopus japonicus were investigated. It was shown that dietary addition of R. benthica D30 significantly increased the growth rates of sea cucumbers (p < 0.05). The amylase activity, cellulase activity and alginase activity were increased for the animals from three probiotics treated groups. And with the supplemented concentration increased, the values of those digestive enzyme activities increased as well. Dietary addition of R. benthica D30 at the level of 10(7) CFU significantly increased the lysozyme, phagocytic and total nitric oxide synthase activity of A. japonicus (p < 0.05). While, the highest values of the phenoloxidase and alkaline phosphatase activity were found in sea cucumbers fed with R. benthica D30 at the level of 10(6) CFU. Whereas adding R. benthica D30 to diet had no significant effects on the total coelomocyte counts and acid phosphatase activity of A. japonicus (p > 0.05). It was observed that adding R. benthica D30 could significantly decrease the cumulative mortality of sea cucumbers. The present study demonstrated that dietary addition of R. benthica D30 could increase growth performance and some digestive enzyme activities, improve immunity and disease resistance of A. japonicus. And the medium (10(6) CFU) and high (10(7) CFU) additional levels showed better effects. It suggests that yeast R. benthica D30 could be a good probiotic for aquaculture. Copyright © 2015 Elsevier Ltd. All rights reserved.

Temperature-Ramped 129Xe Spin-Exchange Optical Pumping

PubMed Central

2015-01-01

We describe temperature-ramped spin-exchange optical pumping (TR-SEOP) in an automated high-throughput batch-mode 129Xe hyperpolarizer utilizing three key temperature regimes: (i) “hot”—where the 129Xe hyperpolarization rate is maximal, (ii) “warm”—where the 129Xe hyperpolarization approaches unity, and (iii) “cool”—where hyperpolarized 129Xe gas is transferred into a Tedlar bag with low Rb content (<5 ng per ∼1 L dose) suitable for human imaging applications. Unlike with the conventional approach of batch-mode SEOP, here all three temperature regimes may be operated under continuous high-power (170 W) laser irradiation, and hyperpolarized 129Xe gas is delivered without the need for a cryocollection step. The variable-temperature approach increased the SEOP rate by more than 2-fold compared to the constant-temperature polarization rate (e.g., giving effective values for the exponential buildup constant γSEOP of 62.5 ± 3.7 × 10–3 min–1 vs 29.9 ± 1.2 × 10–3 min–1) while achieving nearly the same maximum %PXe value (88.0 ± 0.8% vs 90.1% ± 0.8%, for a 500 Torr (67 kPa) Xe cell loading—corresponding to nuclear magnetic resonance/magnetic resonance imaging (NMR/MRI) enhancements of ∼3.1 × 105 and ∼2.32 × 108 at the relevant fields for clinical imaging and HP 129Xe production of 3 T and 4 mT, respectively); moreover, the intercycle “dead” time was also significantly decreased. The higher-throughput TR-SEOP approach can be implemented without sacrificing the level of 129Xe hyperpolarization or the experimental stability for automation—making this approach beneficial for improving the overall 129Xe production rate in clinical settings. PMID:25008290

9 CFR 2.129 - Confiscation and destruction of animals.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 9 Animals and Animal Products 1 2013-01-01 2013-01-01 false Confiscation and destruction of animals. 2.129 Section 2.129 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE ANIMAL WELFARE REGULATIONS Miscellaneous § 2.129 Confiscation and destruction of...

9 CFR 2.129 - Confiscation and destruction of animals.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Confiscation and destruction of animals. 2.129 Section 2.129 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE ANIMAL WELFARE REGULATIONS Miscellaneous § 2.129 Confiscation and destruction of...

9 CFR 2.129 - Confiscation and destruction of animals.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 9 Animals and Animal Products 1 2014-01-01 2014-01-01 false Confiscation and destruction of animals. 2.129 Section 2.129 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE ANIMAL WELFARE REGULATIONS Miscellaneous § 2.129 Confiscation and destruction of...

9 CFR 2.129 - Confiscation and destruction of animals.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 9 Animals and Animal Products 1 2011-01-01 2011-01-01 false Confiscation and destruction of animals. 2.129 Section 2.129 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE ANIMAL WELFARE REGULATIONS Miscellaneous § 2.129 Confiscation and destruction of...

9 CFR 2.129 - Confiscation and destruction of animals.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 9 Animals and Animal Products 1 2012-01-01 2012-01-01 false Confiscation and destruction of animals. 2.129 Section 2.129 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE ANIMAL WELFARE REGULATIONS Miscellaneous § 2.129 Confiscation and destruction of...

7 CFR 4288.122-4288.129 - [Reserved

Code of Federal Regulations, 2013 CFR

2013-01-01

... 7 Agriculture 15 2013-01-01 2013-01-01 false [Reserved] 4288.122-4288.129 Section 4288.122-4288.129 Agriculture Regulations of the Department of Agriculture (Continued) RURAL BUSINESS-COOPERATIVE SERVICE AND RURAL UTILITIES SERVICE, DEPARTMENT OF AGRICULTURE PAYMENT PROGRAMS Advanced Biofuel Payment...

7 CFR 4288.122-4288.129 - [Reserved

Code of Federal Regulations, 2012 CFR

2012-01-01

... 7 Agriculture 15 2012-01-01 2012-01-01 false [Reserved] 4288.122-4288.129 Section 4288.122-4288.129 Agriculture Regulations of the Department of Agriculture (Continued) RURAL BUSINESS-COOPERATIVE SERVICE AND RURAL UTILITIES SERVICE, DEPARTMENT OF AGRICULTURE PAYMENT PROGRAMS Advanced Biofuel Payment...

7 CFR 4288.122-4288.129 - [Reserved

Code of Federal Regulations, 2014 CFR

2014-01-01

... 7 Agriculture 15 2014-01-01 2014-01-01 false [Reserved] 4288.122-4288.129 Section 4288.122-4288.129 Agriculture Regulations of the Department of Agriculture (Continued) RURAL BUSINESS-COOPERATIVE SERVICE AND RURAL UTILITIES SERVICE, DEPARTMENT OF AGRICULTURE PAYMENT PROGRAMS Advanced Biofuel Payment...

27 CFR 24.129 - Change in location.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 27 Alcohol, Tobacco Products and Firearms 1 2011-04-01 2011-04-01 false Change in location. 24.129 Section 24.129 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT... Office of Management and Budget under control number 1512-0058) ...

27 CFR 24.129 - Change in location.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Change in location. 24.129 Section 24.129 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT... Office of Management and Budget under control number 1512-0058) ...

Development of observational learning during school formation in jack mackerel Trachurus japonicus juveniles.

PubMed

Takahashi, Kohji; Masuda, Reiji; Yamashita, Yoh

2014-03-01

We assessed whether the development of observational learning in jack mackerel Trachurus japonicus juveniles corresponds with that of their schooling behaviour. Schooling behaviour was quantitatively analyzed by nearest neighbour distance and separation angle in two size classes of fish, 20-mm and 40-mm in body length. Observer and non-observer fish with matching sizes were conditioned to pellets by temporarily stopping aeration. Observer fish were provided with five observation trials of other individuals feeding near an air stone when aeration was stopped. After the observation trial, fish were conditioned to pellets with the stop of aeration, and then the learning process was evaluated by the increase in the association with the feeding area when aeration was stopped. In 20-mm fish, which were at an immature stage of schooling behaviour, there was no difference in the learning process between observer and non-observer fish. In contrast, 40-mm fish were confirmed to have a well-developed schooling behaviour, and the observer learnt the feeding area more efficiently than the non-observer. This study provides evidence that observational learning develops along with the development of the social interaction. Copyright © 2013 Elsevier B.V. All rights reserved.

Space Shuttle Atlantis Landing / STS-129 Mission

NASA Image and Video Library

2009-11-27

PHOTO CREDIT: NASA or National Aeronautics and Space Administration CAPE CANAVERAL, Fla. - With landing gear down, space shuttle Atlantis approaches landing on Runway 33 at the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida after 11 days in space, completing the 4.5-million mile STS-129 mission on orbit 171. Main gear touchdown was at 9:44:23 a.m. EDT. Nose gear touchdown was at 9:44:36 a.m., and wheels stop was at 9:45:05 a.m. Aboard Atlantis are Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr.; and Expedition 20 and 21 Flight Engineer Nicole Stott who spent 87 days aboard the International Space Station. STS-129 is the final space shuttle Expedition crew rotation flight on the manifest. On STS-129, the crew delivered 14 tons of cargo to the orbiting laboratory, including two ExPRESS Logistics Carriers containing spare parts to sustain station operations after the shuttles are retired next year. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Kim Shiflett

Space Shuttle Atlantis Landing / STS-129 Mission

NASA Image and Video Library

2009-11-27

PHOTO CREDIT: NASA or National Aeronautics and Space Administration CAPE CANAVERAL, Fla. - With drag chute unfurled, space shuttle Atlantis lands on Runway 33 at the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida after 11 days in space, completing the 4.5-million mile STS-129 mission on orbit 171. Main gear touchdown was at 9:44:23 a.m. EDT. Nose gear touchdown was at 9:44:36 a.m., and wheels stop was at 9:45:05 a.m. Aboard Atlantis are Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr.; and Expedition 20 and 21 Flight Engineer Nicole Stott who spent 87 days aboard the International Space Station. STS-129 is the final space shuttle Expedition crew rotation flight on the manifest. On STS-129, the crew delivered 14 tons of cargo to the orbiting laboratory, including two ExPRESS Logistics Carriers containing spare parts to sustain station operations after the shuttles are retired next year. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Kim Shiflett

Space Shuttle Atlantis Landing / STS-129 Mission

NASA Image and Video Library

2009-11-27

PHOTO CREDIT: NASA or National Aeronautics and Space Administration CAPE CANAVERAL, Fla. - The drag chute unfurls to slow space shuttle Atlantis for landing on Runway 33 at the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida after 11 days in space, completing the 4.5-million mile STS-129 mission on orbit 171. Main gear touchdown was at 9:44:23 a.m. EDT. Nose gear touchdown was at 9:44:36 a.m., and wheels stop was at 9:45:05 a.m. Aboard Atlantis are Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr.; and Expedition 20 and 21 Flight Engineer Nicole Stott who spent 87 days aboard the International Space Station. STS-129 is the final space shuttle Expedition crew rotation flight on the manifest. On STS-129, the crew delivered 14 tons of cargo to the orbiting laboratory, including two ExPRESS Logistics Carriers containing spare parts to sustain station operations after the shuttles are retired next year. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Sandra Joseph

Space Shuttle Atlantis Landing / STS-129 Mission

NASA Image and Video Library

2009-11-27

PHOTO CREDIT: NASA or National Aeronautics and Space Administration CAPE CANAVERAL, Fla. - The drag chute unfurls as space shuttle Atlantis lands on Runway 33 at the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida after 11 days in space, completing the 4.5-million mile STS-129 mission on orbit 171. Main gear touchdown was at 9:44:23 a.m. EDT. Nose gear touchdown was at 9:44:36 a.m., and wheels stop was at 9:45:05 a.m. Aboard Atlantis are Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr.; and Expedition 20 and 21 Flight Engineer Nicole Stott who spent 87 days aboard the International Space Station. STS-129 is the final space shuttle Expedition crew rotation flight on the manifest. On STS-129, the crew delivered 14 tons of cargo to the orbiting laboratory, including two ExPRESS Logistics Carriers containing spare parts to sustain station operations after the shuttles are retired next year. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Kim Shiflett

Space Shuttle Atlantis Landing / STS-129 Mission

NASA Image and Video Library

2009-11-27

PHOTO CREDIT: NASA or National Aeronautics and Space Administration CAPE CANAVERAL, Fla. - The drag chute unfurls to slow space shuttle Atlantis for landing on Runway 33 at the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida after 11 days in space, completing the 4.5-million mile STS-129 mission on orbit 171. Main gear touchdown was at 9:44:23 a.m. EDT. Nose gear touchdown was at 9:44:36 a.m., and wheels stop was at 9:45:05 a.m. Aboard Atlantis are Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr.; and Expedition 20 and 21 Flight Engineer Nicole Stott who spent 87 days aboard the International Space Station. STS-129 is the final space shuttle Expedition crew rotation flight on the manifest. On STS-129, the crew delivered 14 tons of cargo to the orbiting laboratory, including two ExPRESS Logistics Carriers containing spare parts to sustain station operations after the shuttles are retired next year. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Jim Grossmann

Space Shuttle Atlantis Landing / STS-129 Mission

NASA Image and Video Library

2009-11-27

PHOTO CREDIT: NASA or National Aeronautics and Space Administration CAPE CANAVERAL, Fla. - Space shuttle Atlantis kicks up dust as it touches down on Runway 33 at the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida after 11 days in space, completing the 4.5-million mile STS-129 mission on orbit 171. Main gear touchdown was at 9:44:23 a.m. EDT. Nose gear touchdown was at 9:44:36 a.m., and wheels stop was at 9:45:05 a.m. Aboard Atlantis are Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr.; and Expedition 20 and 21 Flight Engineer Nicole Stott who spent 87 days aboard the International Space Station. STS-129 is the final space shuttle Expedition crew rotation flight on the manifest. On STS-129, the crew delivered 14 tons of cargo to the orbiting laboratory, including two ExPRESS Logistics Carriers containing spare parts to sustain station operations after the shuttles are retired next year. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Kim Shiflett

Space Shuttle Atlantis Landing / STS-129 Mission

NASA Image and Video Library

2009-11-27

PHOTO CREDIT: NASA or National Aeronautics and Space Administration CAPE CANAVERAL, Fla. - Streams of smoke trail from the main landing gear tires as space shuttle Atlantis touches down on Runway 33 at the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida after 11 days in space, completing the 4.5-million-mile STS-129 mission on orbit 171. Main gear touchdown was at 9:44:23 a.m. EDT. Nose gear touchdown was at 9:44:36 a.m., and wheels stop was at 9:45:05 a.m. Aboard Atlantis are Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr.; and Expedition 20 and 21 Flight Engineer Nicole Stott who spent 87 days aboard the International Space Station. STS-129 is the final space shuttle Expedition crew rotation flight on the manifest. On STS-129, the crew delivered 14 tons of cargo to the orbiting laboratory, including two ExPRESS Logistics Carriers containing spare parts to sustain station operations after the shuttles are retired next year. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Jim Grossmann

Space Shuttle Atlantis Landing / STS-129 Mission

NASA Image and Video Library

2009-11-27

PHOTO CREDIT: NASA or National Aeronautics and Space Administration CAPE CANAVERAL, Fla. - A fire and rescue truck is in place beside Runway 33 if needed to support the landing of space shuttle Atlantis at the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida. After 11 days in space, Atlantis completed the 4.5-million mile STS-129 mission on orbit 171. Main gear touchdown was at 9:44:23 a.m. EDT. Nose gear touchdown was at 9:44:36 a.m., and wheels stop was at 9:45:05 a.m. Aboard Atlantis are Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr.; and Expedition 20 and 21 Flight Engineer Nicole Stott who spent 87 days aboard the International Space Station. STS-129 is the final space shuttle Expedition crew rotation flight on the manifest. On STS-129, the crew delivered 14 tons of cargo to the orbiting laboratory, including two ExPRESS Logistics Carriers containing spare parts to sustain station operations after the shuttles are retired next year. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Jack Pfaller

The glutathione peroxidase gene family of Lotus japonicus: characterization of genomic clones, expression analyses and immunolocalization in legumes.

PubMed

Ramos, Javier; Matamoros, Manuel A; Naya, Loreto; James, Euan K; Rouhier, Nicolas; Sato, Shusei; Tabata, Satoshi; Becana, Manuel

2009-01-01

Despite the multiple roles played by antioxidants in rhizobia-legume symbioses, little is known about glutathione peroxidases (GPXs) in legumes. Here the characterization of six GPX genes of Lotus japonicus is reported. Expression of GPX genes was analysed by quantitative reverse transcription-polymerase chain reaction in L. japonicus and Lotus corniculatus plants exposed to various treatments known to generate reactive oxygen and/or nitrogen species. LjGPX1 and LjGPX3 were the most abundantly expressed genes in leaves, roots and nodules. Compared with roots, LjGPX1 and LjGPX6 were highly expressed in leaves and LjGPX3 and LjGPX6 in nodules. In roots, salinity decreased GPX4 expression, aluminium decreased expression of the six genes, and cadmium caused up-regulation of GPX3, GPX4 and GPX5 after 1 h and down-regulation of GPX1, GPX2, GPX4 and GPX6 after 3-24 h. Exposure of roots to sodium nitroprusside (a nitric oxide donor) for 1 h increased the mRNA levels of GPX4 and GPX6 by 3.3- and 30-fold, respectively. Thereafter, the GPX6 mRNA level remained consistently higher than that of the control. Immunogold labelling revealed the presence of GPX proteins in root and nodule amyloplasts and in leaf chloroplasts of L. japonicus and other legumes. Labelling was associated with starch grains. These results underscore the differential regulation of GPX expression in response to cadmium, aluminium and nitric oxide, and strongly support a role for GPX6 and possibly other GPX genes in stress and/or metabolic signalling.

22 CFR 1600.112-1600.129 - [Reserved

Code of Federal Regulations, 2013 CFR

2013-04-01

... 22 Foreign Relations 2 2013-04-01 2009-04-01 true [Reserved] 1600.112-1600.129 Section 1600.112-1600.129 Foreign Relations JAPAN-UNITED STATES FRIENDSHIP COMMISSION ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE JAPAN-UNITED STATES FRIENDSHIP...

22 CFR 1600.112-1600.129 - [Reserved

Code of Federal Regulations, 2012 CFR

2012-04-01

... 22 Foreign Relations 2 2012-04-01 2009-04-01 true [Reserved] 1600.112-1600.129 Section 1600.112-1600.129 Foreign Relations JAPAN-UNITED STATES FRIENDSHIP COMMISSION ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE JAPAN-UNITED STATES FRIENDSHIP...

22 CFR 1600.112-1600.129 - [Reserved

Code of Federal Regulations, 2014 CFR

2014-04-01

... 22 Foreign Relations 2 2014-04-01 2014-04-01 false [Reserved] 1600.112-1600.129 Section 1600.112-1600.129 Foreign Relations JAPAN-UNITED STATES FRIENDSHIP COMMISSION ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE JAPAN-UNITED STATES FRIENDSHIP...

22 CFR 1600.112-1600.129 - [Reserved

Code of Federal Regulations, 2011 CFR

2011-04-01

... 22 Foreign Relations 2 2011-04-01 2009-04-01 true [Reserved] 1600.112-1600.129 Section 1600.112-1600.129 Foreign Relations JAPAN-UNITED STATES FRIENDSHIP COMMISSION ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE JAPAN-UNITED STATES FRIENDSHIP...

46 CFR 507.112-507.129 - [Reserved

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 9 2011-10-01 2011-10-01 false [Reserved] 507.112-507.129 Section 507.112-507.129 Shipping FEDERAL MARITIME COMMISSION GENERAL AND ADMINISTRATIVE PROVISIONS ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE FEDERAL MARITIME COMMISSION...

46 CFR 507.112-507.129 - [Reserved

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 9 2014-10-01 2014-10-01 false [Reserved] 507.112-507.129 Section 507.112-507.129 Shipping FEDERAL MARITIME COMMISSION GENERAL AND ADMINISTRATIVE PROVISIONS ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE FEDERAL MARITIME COMMISSION...

22 CFR 1103.112-1103.129 - [Reserved

Code of Federal Regulations, 2010 CFR

2010-04-01

... 22 Foreign Relations 2 2010-04-01 2010-04-01 true [Reserved] 1103.112-1103.129 Section 1103.112-1103.129 Foreign Relations INTERNATIONAL BOUNDARY AND WATER COMMISSION, UNITED STATES AND MEXICO... CONDUCTED BY INTERNATIONAL BOUNDARY AND WATER COMMISSION, UNITED STATES AND MEXICO, UNITED STATES SECTION...

22 CFR 1600.112-1600.129 - [Reserved

Code of Federal Regulations, 2010 CFR

2010-04-01

... 22 Foreign Relations 2 2010-04-01 2010-04-01 true [Reserved] 1600.112-1600.129 Section 1600.112-1600.129 Foreign Relations JAPAN-UNITED STATES FRIENDSHIP COMMISSION ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE JAPAN-UNITED STATES FRIENDSHIP...

34 CFR 1200.112-1200.129 - [Reserved

Code of Federal Regulations, 2010 CFR

2010-07-01

... 34 Education 3 2010-07-01 2010-07-01 false [Reserved] 1200.112-1200.129 Section 1200.112-1200.129 Education Regulations of the Offices of the Department of Education (Continued) NATIONAL COUNCIL ON DISABILITY ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY...

46 CFR 129.550 - Power for cooking and heating.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 4 2010-10-01 2010-10-01 false Power for cooking and heating. 129.550 Section 129.550... INSTALLATIONS Miscellaneous Electrical Systems § 129.550 Power for cooking and heating. (a) Equipment for cooking and heating must be suitable for marine use. Equipment designed and installed to comply with ABYC...

Launch of Space Shuttle Atlantis / STS-129 Mission

NASA Image and Video Library

2009-11-16

CAPE CANAVERAL, Fla. - Space shuttle Atlantis launches through the clouds from Launch Pad 39A on a balmy Florida afternoon at NASA's Kennedy Space Center. Liftoff on its STS-129 mission came at 2:28 p.m. EST Nov. 16. Aboard are crew members Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; and Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr. On STS-129, the crew will deliver two Express Logistics Carriers to the International Space Station, the largest of the shuttle's cargo carriers, containing 15 spare pieces of equipment including two gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. Atlantis will return to Earth a station crew member, Nicole Stott, who has spent more than two months aboard the orbiting laboratory. STS-129 is slated to be the final space shuttle Expedition crew rotation flight. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Jim Grossmann

Launch of Space Shuttle Atlantis / STS-129 Mission

NASA Image and Video Library

2009-11-16

CAPE CANAVERAL, Fla. - Space shuttle Atlantis cuts its way through the blue skies over Launch Pad 39A at NASA's Kennedy Space Center in Florida. Liftoff on its STS-129 mission came at 2:28 p.m. EST Nov. 16. Aboard are crew members Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; and Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr. On STS-129, the crew will deliver two Express Logistics Carriers to the International Space Station, the largest of the shuttle's cargo carriers, containing 15 spare pieces of equipment including two gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. Atlantis will return to Earth a station crew member, Nicole Stott, who has spent more than two months aboard the orbiting laboratory. STS-129 is slated to be the final space shuttle Expedition crew rotation flight. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Jim Grossmann

5 CFR 1850.112-1850.129 - [Reserved

Code of Federal Regulations, 2010 CFR

2010-01-01

... 5 Administrative Personnel 3 2010-01-01 2010-01-01 false [Reserved] 1850.112-1850.129 Section 1850.112-1850.129 Administrative Personnel OFFICE OF SPECIAL COUNSEL ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE OFFICE OF SPECIAL COUNSEL §§ 1850.112...

Sesquiterpene furan compound CJ-01, a novel chitin synthase 2 inhibitor from Chloranthus japonicus SIEB.

PubMed

Yim, Nam Hui; Hwang, Eui Il; Yun, Bong Sik; Park, Ki Duk; Moon, Jae Sun; Lee, Sang Han; Sung, Nack Do; Kim, Sung Uk

2008-05-01

A novel sesquiterpene furan compound CJ-01 was isolated from the methanol extract of the whole plant of Chloranthus japonicus SIEB. by monitoring the inhibitory activity of chitin synthase 2 from Saccharomyces cerevisiae. Based on spectroscopic analysis, the structure of compound CJ-01 was determined as 3,4,8a-trimethyl-4a,7,8,8a-tetrahydro-4a-naphto[2,3-b]furan-9-one. The compound inhibited chitin synthase 2 of Saccharomyces cerevisiae in a dose-dependent manner with an IC50 of 39.6 microg/ml, whereas it exhibited no inhibitory activities against chitin synthase 1 and 3 of S. cerevisiae up to 280 microg/ml. CJ-01 has 1.7-fold stronger inhibitory activity than polyoxin D (IC50=70 microg/ml), a well-known chitin synthase inhibitor. These results indicate that the compound is a specific inhibitor of chitin synthase 2 from S. cerevisiae. In addition, CJ-01 showed antifungal activities against various human and phytopathogenic fungi. Therefore, the compound might be an interesting lead to develop effective antifungal agents.

Proteolysis of noncollagenous proteins in sea cucumber, Stichopus japonicus, body wall: characterisation and the effects of cysteine protease inhibitors.

PubMed

Wu, Hai-Tao; Li, Dong-Mei; Zhu, Bei-Wei; Sun, Jin-Jian; Zheng, Jie; Wang, Feng-Lin; Konno, Kunihiko; Jiang, Xi

2013-11-15

Proteolysis of noncollagenous proteins in sea cucumber, Stichopus Japonicus, body wall (sjBW) was investigated. The proteins removed from sjBW by SDS and urea extraction were mainly noncollagenous proteins with molecular weights about 200kDa (Band I) and 44kDa (Band II), respectively. Band I and Band II were identified as major yolk protein (MYP) and actin, respectively, from holothurian species by liquid chromatography-mass spectrometry (LC-MS/MS) with significant scores. Based on TCA-soluble oligopeptide assay, the optimum proteolysis condition of noncollagenous proteins was at 46.3°C and pH 6.1, by response surface methodology. The proteolysis of MYP, and actin, was partially inhibited by cysteine protease inhibitors, including Trans-epoxysuccinyl-l-leucyl-amido (4-guanidino) butane (E-64), iodoacetic acid, antipain and whey protein concentrate. These results suggest that cysteine proteases are partially involved in the proteolysis of noncollagenous proteins in body wall of sea cucumber, S. japonicus. Copyright © 2013 Elsevier Ltd. All rights reserved.

22 CFR 144.112-144.129 - [Reserved

Code of Federal Regulations, 2011 CFR

2011-04-01

... 22 Foreign Relations 1 2011-04-01 2011-04-01 false [Reserved] 144.112-144.129 Section 144.112-144.129 Foreign Relations DEPARTMENT OF STATE CIVIL RIGHTS ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE UNITED STATES DEPARTMENT OF STATE §§ 144.112...

22 CFR 144.112-144.129 - [Reserved

Code of Federal Regulations, 2014 CFR

2014-04-01

... 22 Foreign Relations 1 2014-04-01 2014-04-01 false [Reserved] 144.112-144.129 Section 144.112-144.129 Foreign Relations DEPARTMENT OF STATE CIVIL RIGHTS ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE UNITED STATES DEPARTMENT OF STATE §§ 144.112...

22 CFR 144.112-144.129 - [Reserved

Code of Federal Regulations, 2013 CFR

2013-04-01

... 22 Foreign Relations 1 2013-04-01 2013-04-01 false [Reserved] 144.112-144.129 Section 144.112-144.129 Foreign Relations DEPARTMENT OF STATE CIVIL RIGHTS ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE UNITED STATES DEPARTMENT OF STATE §§ 144.112...

22 CFR 144.112-144.129 - [Reserved

Code of Federal Regulations, 2010 CFR

2010-04-01

... 22 Foreign Relations 1 2010-04-01 2010-04-01 false [Reserved] 144.112-144.129 Section 144.112-144.129 Foreign Relations DEPARTMENT OF STATE CIVIL RIGHTS ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE UNITED STATES DEPARTMENT OF STATE §§ 144.112...

22 CFR 144.112-144.129 - [Reserved

Code of Federal Regulations, 2012 CFR

2012-04-01

... 22 Foreign Relations 1 2012-04-01 2012-04-01 false [Reserved] 144.112-144.129 Section 144.112-144.129 Foreign Relations DEPARTMENT OF STATE CIVIL RIGHTS ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE UNITED STATES DEPARTMENT OF STATE §§ 144.112...

38 CFR 21.129 - Home study course.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 38 Pensions, Bonuses, and Veterans' Relief 2 2010-07-01 2010-07-01 false Home study course. 21.129... Educational and Vocational Training Services § 21.129 Home study course. (a) Definition. A home study course... school to the student for study and preparation of written answers, solutions to problems, and work...

38 CFR 21.129 - Home study course.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 38 Pensions, Bonuses, and Veterans' Relief 2 2011-07-01 2011-07-01 false Home study course. 21.129... Educational and Vocational Training Services § 21.129 Home study course. (a) Definition. A home study course... school to the student for study and preparation of written answers, solutions to problems, and work...

129. Photographic copy of drawing (24 June 1963, original drawing ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

129. Photographic copy of drawing (24 June 1963, original drawing in Archives, Public Affairs Departmentm, Sears Merchandise Group, Hoffman Estates, Illinois). Swain and Meyers, Inc. 1/4 inch to one foot. Partial floor plan illustrating the layout of the large package handling area. Tables and equipment not present at the time of the survey are shown. SECOND FLOOR, SECTON B - Sears Roebuck & Company Mail Order Plant, Merchandise Building, 924 South Homan Avenue, Chicago, Cook County, IL

38 CFR 21.129 - Home study course.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 38 Pensions, Bonuses, and Veterans' Relief 2 2012-07-01 2012-07-01 false Home study course. 21.129... Educational and Vocational Training Services § 21.129 Home study course. (a) Definition. A home study course... inclusion of home study courses, in rehabilitation plans. A veteran and his or her case manager may include...

38 CFR 21.129 - Home study course.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 38 Pensions, Bonuses, and Veterans' Relief 2 2014-07-01 2014-07-01 false Home study course. 21.129... Educational and Vocational Training Services § 21.129 Home study course. (a) Definition. A home study course... inclusion of home study courses, in rehabilitation plans. A veteran and his or her case manager may include...

38 CFR 21.129 - Home study course.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 38 Pensions, Bonuses, and Veterans' Relief 2 2013-07-01 2013-07-01 false Home study course. 21.129... Educational and Vocational Training Services § 21.129 Home study course. (a) Definition. A home study course... inclusion of home study courses, in rehabilitation plans. A veteran and his or her case manager may include...

Bioenergetic trade-offs in the sea cucumber Apostichopus japonicus (Echinodermata: Holothuroidea) in response to CO2-driven ocean acidification.

PubMed

Yuan, Xiutang; Shao, Senlin; Yang, Xiaolong; Yang, Dazuo; Xu, Qinzeng; Zong, Humin; Liu, Shilin

2016-05-01

Ocean acidification (OA) caused by excessive CO2 is a potential ecological threat to marine organisms. The impacts of OA on echinoderms are well-documented, but there has been a strong bias towards sea urchins, and limited information is available on sea cucumbers. This work examined the effect of medium-term (60 days) exposure to three pH levels (pH 8.06, 7.72, and 7.41, covering present and future pH variability) on the bioenergetic responses of the sea cucumber, Apostichopus japonicus, an ecologically and economically important holothurian in Asian coasts. Results showed that the measured specific growth rate linearly decreased with decreased pH, leading to a 0.42 %·day(-1) decrease at pH 7.41 compared with that at pH 8.06. The impacts of pH on physiological energetics were variable: measured energy consumption and defecation rates linearly decreased with decreased pH, whereas maintenance energy in calculated respiration and excretion were not significantly affected. No shift in energy allocation pattern was observed in A. japonicus upon exposure to pH 7.72 compared with pH 8.06. However, a significant shift in energy budget occurred upon exposure to pH 7.41, leading to decreased energy intake and increased percentage of energy that was lost in feces, thereby resulting in a significantly lowered allocation into somatic growth. These findings indicate that adult A. japonicus is resilient to the OA scenario at the end of the twenty-first century, but further acidification may negatively influence the grazing capability and growth, thereby influencing its ecological functioning as an "ecosystem engineer" and potentially harming its culture output.

12 CFR 410.112-410.129 - [Reserved

Code of Federal Regulations, 2010 CFR

2010-01-01

... 12 Banks and Banking 4 2010-01-01 2010-01-01 false [Reserved] 410.112-410.129 Section 410.112-410.129 Banks and Banking EXPORT-IMPORT BANK OF THE UNITED STATES ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY EXPORT-IMPORT BANK OF THE UNITED STATES §§ 410...

Launch of Space Shuttle Atlantis / STS-129 Mission

NASA Image and Video Library

2009-11-16

CAPE CANAVERAL, Fla. - With nearly 7 million pounds of thrust generated by twin solid rocket boosters and three main engines, space shuttle Atlantis zooms into the blue skies over Launch Pad 39A at NASA's Kennedy Space Center in Florida. Liftoff on its STS-129 mission came at 2:28 p.m. EST Nov. 16. Aboard are crew members Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; and Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr. On STS-129, the crew will deliver two ExPRESS Logistics Carriers to the International Space Station, the largest of the shuttle's cargo carriers, containing 15 spare pieces of equipment including two gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. Atlantis will return to Earth a station crew member, Nicole Stott, who has spent more than two months aboard the orbiting laboratory. STS-129 is slated to be the final space shuttle Expedition crew rotation flight. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Kenny Allen

Launch of Space Shuttle Atlantis / STS-129 Mission

NASA Image and Video Library

2009-11-16

CAPE CANAVERAL, Fla. - Twitter followers and media representatives at the NASA Press Site witness space shuttle Atlantis cut its way through the blue skies over Launch Pad 39A at NASA's Kennedy Space Center in Florida. Liftoff on its STS-129 mission came at 2:28 p.m. EST Nov. 16. Aboard are crew members Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; and Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr. On STS-129, the crew will deliver two Express Logistics Carriers to the International Space Station, the largest of the shuttle's cargo carriers, containing 15 spare pieces of equipment including two gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. Atlantis will return to Earth a station crew member, Nicole Stott, who has spent more than two months aboard the orbiting laboratory. STS-129 is slated to be the final space shuttle Expedition crew rotation flight. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Gianni Woods

Launch of Space Shuttle Atlantis / STS-129 Mission

NASA Image and Video Library

2009-11-16

CAPE CANAVERAL, Fla. - With nearly 7 million pounds of thrust generated by twin solid rocket boosters and three main engines, space shuttle Atlantis races to orbit over Launch Pad 39A at NASA's Kennedy Space Center in Florida. Liftoff on its STS-129 mission came at 2:28 p.m. EST Nov. 16. Aboard are crew members Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; and Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr. On STS-129, the crew will deliver two ExPRESS Logistics Carriers to the International Space Station, the largest of the shuttle's cargo carriers, containing 15 spare pieces of equipment including two gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. Atlantis will return to Earth a station crew member, Nicole Stott, who has spent more than two months aboard the orbiting laboratory. STS-129 is slated to be the final space shuttle Expedition crew rotation flight. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Kenny Allen

Launch of Space Shuttle Atlantis / STS-129 Mission

NASA Image and Video Library

2009-11-16

CAPE CANAVERAL, Fla. - Twitter followers and media representatives at the NASA Press Site watch as space shuttle Atlantis springs into action from Launch Pad 39A at NASA's Kennedy Space Center in Florida. Liftoff on its STS-129 mission came at 2:28 p.m. EST Nov. 16. Aboard are crew members Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; and Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr. On STS-129, the crew will deliver two Express Logistics Carriers to the International Space Station, the largest of the shuttle's cargo carriers, containing 15 spare pieces of equipment including two gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. Atlantis will return to Earth a station crew member, Nicole Stott, who has spent more than two months aboard the orbiting laboratory. STS-129 is slated to be the final space shuttle Expedition crew rotation flight. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Gianni Woods

Launch of Space Shuttle Atlantis / STS-129 Mission

NASA Image and Video Library

2009-11-16

CAPE CANAVERAL, Fla. - Like a phoenix rising from the flames, space shuttle Atlantis takes flight from Launch Pad 39A at NASA's Kennedy Space Center in Florida. Liftoff on its STS-129 mission came at 2:28 p.m. EST Nov. 16. Aboard are crew members Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; and Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr. On STS-129, the crew will deliver two Express Logistics Carriers to the International Space Station, the largest of the shuttle's cargo carriers, containing 15 spare pieces of equipment including two gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. Atlantis will return to Earth a station crew member, Nicole Stott, who has spent more than two months aboard the orbiting laboratory. STS-129 is slated to be the final space shuttle Expedition crew rotation flight. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Jim Grossmann

Launch of Space Shuttle Atlantis / STS-129 Mission

NASA Image and Video Library

2009-11-16

CAPE CANAVERAL, Fla. - Twitter followers and media representatives at the NASA Press Site have front-row seats as space shuttle Atlantis launches through the clouds from Launch Pad 39A on a balmy Florida afternoon at NASA's Kennedy Space Center. Liftoff on its STS-129 mission came at 2:28 p.m. EST Nov. 16. Aboard are crew members Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; and Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr. On STS-129, the crew will deliver two Express Logistics Carriers to the International Space Station, the largest of the shuttle's cargo carriers, containing 15 spare pieces of equipment including two gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. Atlantis will return to Earth a station crew member, Nicole Stott, who has spent more than two months aboard the orbiting laboratory. STS-129 is slated to be the final space shuttle Expedition crew rotation flight. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Gianni Woods

Launch of Space Shuttle Atlantis / STS-129 Mission

NASA Image and Video Library

2009-11-16

CAPE CANAVERAL, Fla. - An exhaust cloud begins to form around space shuttle Atlantis as it springs into action from Launch Pad 39A at NASA's Kennedy Space Center in Florida. Liftoff on its STS-129 mission came at 2:28 p.m. EST Nov. 16. Aboard are crew members Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; and Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr. On STS-129, the crew will deliver two Express Logistics Carriers to the International Space Station, the largest of the shuttle's cargo carriers, containing 15 spare pieces of equipment including two gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. Atlantis will return to Earth a station crew member, Nicole Stott, who has spent more than two months aboard the orbiting laboratory. STS-129 is slated to be the final space shuttle Expedition crew rotation flight. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit:Jim Grossmann

Prion Protein M129V Polymorphism Affects Retrieval-Related Brain Activity

ERIC Educational Resources Information Center

Buchmann, Andreas; Mondadori, Christian R. A.; Hanggi, Jurgen; Aerni, Amanda; Vrticka, Pascal; Luechinger, Roger; Boesiger, Peter; Hock, Christoph; Nitsch, Roger M.; de Quervain, Dominique J.-F.; Papassotiropoulos, Andreas; Henke, Katharina

2008-01-01

The prion protein Met129Val polymorphism has recently been related to human long-term memory with carriers of either the 129[superscript MM] or the 129[superscript MV] genotype recalling 17% more words than 129[superscript VV] carriers at 24 h following learning. Here, we sampled genotype differences in retrieval-related brain activity at 30 min…

10 CFR 1.29 - Office of International Programs.

Code of Federal Regulations, 2013 CFR

2013-01-01

... maintains working relationships with individual countries and international nuclear organizations, as well... 10 Energy 1 2013-01-01 2013-01-01 false Office of International Programs. 1.29 Section 1.29 Energy NUCLEAR REGULATORY COMMISSION STATEMENT OF ORGANIZATION AND GENERAL INFORMATION Headquarters Commission...

10 CFR 1.29 - Office of International Programs.

Code of Federal Regulations, 2010 CFR

2010-01-01

... maintains working relationships with individual countries and international nuclear organizations, as well... 10 Energy 1 2010-01-01 2010-01-01 false Office of International Programs. 1.29 Section 1.29 Energy NUCLEAR REGULATORY COMMISSION STATEMENT OF ORGANIZATION AND GENERAL INFORMATION Headquarters Commission...

10 CFR 1.29 - Office of International Programs.

Code of Federal Regulations, 2011 CFR

2011-01-01

... maintains working relationships with individual countries and international nuclear organizations, as well... 10 Energy 1 2011-01-01 2011-01-01 false Office of International Programs. 1.29 Section 1.29 Energy NUCLEAR REGULATORY COMMISSION STATEMENT OF ORGANIZATION AND GENERAL INFORMATION Headquarters Commission...

10 CFR 1.29 - Office of International Programs.

Code of Federal Regulations, 2012 CFR

2012-01-01

... maintains working relationships with individual countries and international nuclear organizations, as well... 10 Energy 1 2012-01-01 2012-01-01 false Office of International Programs. 1.29 Section 1.29 Energy NUCLEAR REGULATORY COMMISSION STATEMENT OF ORGANIZATION AND GENERAL INFORMATION Headquarters Commission...

10 CFR 1.29 - Office of International Programs.

Code of Federal Regulations, 2014 CFR

2014-01-01

... maintains working relationships with individual countries and international nuclear organizations, as well... 10 Energy 1 2014-01-01 2014-01-01 false Office of International Programs. 1.29 Section 1.29 Energy NUCLEAR REGULATORY COMMISSION STATEMENT OF ORGANIZATION AND GENERAL INFORMATION Headquarters Commission...

Molecular characterization of muscle-parasitizing didymozoid from a chub mackerel, Scomber japonicus.

PubMed

Abe, Niichiro; Okamoto, Mitsuru

2015-09-01

Didymozoids found in the muscles of marine fish are almost always damaged because they are usually found after being sliced. Therefore, identifying muscle-parasitizing didymozoids is difficult because of the difficulty in collecting non-damaged worms and observing their organs as key points for morphological identification. Moreover, muscle-parasitizing didymozoids are not easily found because they parasitize at the trunk muscles. Therefore, muscle-parasitizing didymozoid classification has not progressed because there are few opportunities to detect them. Our recent report was the first to describe the usefulness of sequencing analysis for discrimination among muscle-parasitizing didymozoids. Recently, we found a didymozoid in the trunk muscle of a chub mackerel Scomber japonicus. The present study genetically compares the present isolate with other muscle-parasitizing didymozoids. The present isolate differs markedly from the previously unidentified didymozoid from an Atlantic mackerel S. scombrus by phylogenetic analysis of 18S rDNA. It also differs from other muscle-parasitizing didymozoids from other host species based on phylogenetic analyses of 18S, 28S rDNAs, and coxI loci. These results suggest that sequencing analysis is useful for the discrimination of muscle-parasitizing didymozoids. Combining the present data with earlier data for sequencing analysis, muscle-parasitizing didymozoids from seven marine fish species were classified as seven species. We proposed appellations for six distinct muscle-parasitizing didymozoids for future analysis: sweetlips fish type from Diagramma pictum and Plectorhinchus cinctus, red sea bream type from Pagrus major, flying fish type from Cypselurus heterurus, Atlantic mackerel type from Scomber scombrus, chub mackerel type from S. japonicus, and purple rockcod type from Epinephelus cyanopodus.

Structural and biochemical changes in dermis of sea cucumber (Stichopus japonicus) during autolysis in response to cutting the body wall.

PubMed

Liu, Yu-Xin; Zhou, Da-Yong; Liu, Zi-Qiang; Lu, Ting; Song, Liang; Li, Dong-Mei; Dong, Xiu-Ping; Qi, Hang; Zhu, Bei-Wei; Shahidi, Fereidoon

2018-02-01

The autolysis of sea cucumber body wall is caused by endogenous proteolysis of its structural elements. However, changes in collagen fibrils, collagen fibres and microfibrils, the major structural elements in sea cucumber body wall during autolysis are less clear. Autolysis of sea cucumber (S. japonicus) was induced by cutting the body wall, and the structural and biochemical changes in its dermis were investigated using electron microscopy, differential scanning calorimetry, infrared spectroscopy, electrophoresis, and chemical analysis. During autolysis, both collagen fibres and microfibrils gradually degraded. In contrast, damage to microfibrils was more pronounced. Upon massive autolysis, collagen fibres disaggregated into collagen fibril bundles and individual fibrils due to the fracture of interfibrillar bridges. Meanwhile, excessive unfolding of collagen fibrils occurred. However, there was only slight damage to collagen monomers. Therefore, structural damage in collagen fibres, collagen fibrils and microfibrils rather than monomeric collagen accounts for autolysis of S. japonicus dermis. Copyright © 2017 Elsevier Ltd. All rights reserved.

7 CFR 762.129 - Percent of guarantee and maximum loss.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 7 Agriculture 7 2014-01-01 2014-01-01 false Percent of guarantee and maximum loss. 762.129 Section 762.129 Agriculture Regulations of the Department of Agriculture (Continued) FARM SERVICE AGENCY, DEPARTMENT OF AGRICULTURE SPECIAL PROGRAMS GUARANTEED FARM LOANS § 762.129 Percent of guarantee and maximum...

14 CFR 129.201 - SFAR No. 111-Lavatory Oxygen Systems.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 14 Aeronautics and Space 3 2014-01-01 2014-01-01 false SFAR No. 111-Lavatory Oxygen Systems. 129.201 Section 129.201 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF... Special Federal Aviation Regulations § 129.201 SFAR No. 111—Lavatory Oxygen Systems. The requirements of...

14 CFR 129.201 - SFAR No. 111-Lavatory Oxygen Systems.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 14 Aeronautics and Space 3 2013-01-01 2013-01-01 false SFAR No. 111-Lavatory Oxygen Systems. 129.201 Section 129.201 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF... Special Federal Aviation Regulations § 129.201 SFAR No. 111—Lavatory Oxygen Systems. The requirements of...

14 CFR 129.201 - SFAR No. 111-Lavatory Oxygen Systems.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 14 Aeronautics and Space 3 2012-01-01 2012-01-01 false SFAR No. 111-Lavatory Oxygen Systems. 129.201 Section 129.201 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF... Special Federal Aviation Regulations § 129.201 SFAR No. 111—Lavatory Oxygen Systems. The requirements of...

[Comparative analyze on hepatitis B seroepidemiological surveys among population aged 1-29 years in different epidemic regions of China in 1992 and 2014].

PubMed

Wang, F Z; Zhang, G M; Shen, L P; Zheng, H; Wang, F; Miao, N; Yuan, Q L; Sun, X J; Bi, S L; Liang, X F; Wang, H Q

2017-06-06

Objective: To evaluate the effect of hepatitis B prevention and control by comparative analysis on the results of HBsAg, anti-HBs and anti-HBc prevalence from national hepatitis B seroepidemiological surveys in 1992 and 2014 in different epidemic regions of China. Methods: Data was from the national seroepidemiological surveys of hepatitis B conducted in 1992 and 2014. The survey in 1992 was conducted in 145 disease surveillance points of 30 provinces (excluding Hong Kong, Macao Special Administrative Region and Taiwan province) in China. The survey in 2016 was conducted in 160 disease surveillance points of 31 provinces (excluding Hong Kong, Macao Special Administrative Region and Taiwan province) in China. In the two surveys, face-to-face interviews with the subject by door to door or on the investigation site were conducted by trained staff using standard questionnaires to obtain basic information including birth date, gender, ethnicity, resident place and so on. And then 5 ml venous blood was collected to test the sero-markers of HBsAg, anti-HBs and anti-HBc. We analyzed unweighted point prevalence and 95 % CI of HBsAg, anti-HBs and anti-HBc in 1992 which had no design weighting, and analyzed weighted point prevalence and 95 %CI of HBsAg, anti-HBs and anti-HBc in 2014 which had design weighting. Results: 34 291 and 31 713 people aged 1-29 years were involved in 1992 and 2014 national serosurveys of China, respectively. For the people aged 1-29 years, HBsAg prevalence was 2.64% (95 %CI: 2.28%-3.06%) in 2014 and decreased by 73.92% as compared with the rate 10.13% (95 % CI: 9.81%-10.45%) in 1992. Anti-HBc prevalence was 13.01% (95 %CI: 12.09%-14.00%) in 2014 and decreased by 71.61% as compared with the rate 45.84% (95 % CI: 45.31%-46.37%) in 1992. Anti-HBs prevalence was 57.79% (95 %CI: 56.33%-59.25%) in 2014 and ascended by 127.41% as compared with the rate 25.41% (95 % CI: 24.95%-25.87%) in 1992. In high, medium and low epidemic region, for the people who born