46 CFR Sec. 2 - Bank account.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 8 2011-10-01 2011-10-01 false Bank account. Sec. 2 Section 2 Shipping MARITIME... TRANSACTIONS UNDER AGENCY AGREEMENTS Accounts Sec. 2 Bank account. A separate joint bank account will be... the depository or depositories, the owner will issue an order for the establishment of the joint bank...

46 CFR Sec. 2 - Bank account.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 8 2010-10-01 2010-10-01 false Bank account. Sec. 2 Section 2 Shipping MARITIME... TRANSACTIONS UNDER AGENCY AGREEMENTS Accounts Sec. 2 Bank account. A separate joint bank account will be... the depository or depositories, the owner will issue an order for the establishment of the joint bank...

75 FR 17417 - Joint Meeting of the Arthritis Advisory Committee and the Drug Safety and Risk Management...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-06

...] Joint Meeting of the Arthritis Advisory Committee and the Drug Safety and Risk Management Advisory... Arthritis Advisory Committee and the Drug Safety and Risk Management Advisory Committee. This meeting was... Drug Safety and Risk Management Advisory Committee would be held on May 12, 2010. On page 10490, in the...

75 FR 32189 - Joint Meeting of the Endocrinologic and Metabolic Drugs Advisory Committee and the Drug Safety...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-07

... Drugs Advisory Committee and the Drug Safety and Risk Management Advisory Committee. General Function of...] Joint Meeting of the Endocrinologic and Metabolic Drugs Advisory Committee and the Drug Safety and Risk Management Advisory Committee; Notice of Meeting AGENCY: Food and Drug Administration, HHS. ACTION: Notice...

78 FR 22270 - Joint Meeting of the Endocrinologic and Metabolic Drugs Advisory Committee and the Drug Safety...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-04-15

... Drugs Advisory Committee and the Drug Safety and Risk Management Advisory Committee. General Function of...] Joint Meeting of the Endocrinologic and Metabolic Drugs Advisory Committee and the Drug Safety and Risk Management Advisory Committee; Notice of Meeting AGENCY: Food and Drug Administration, HHS. ACTION: Notice...

76 FR 19374 - Joint Meeting of the Cardiovascular and Renal Drugs Advisory Committee and the Drug Safety and...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-07

...: Cardiovascular and Renal Drugs Advisory Committee and the Drug Safety and Risk Management Advisory Committee...] Joint Meeting of the Cardiovascular and Renal Drugs Advisory Committee and the Drug Safety and Risk Management Advisory Committee; Notice of Meeting AGENCY: Food and Drug Administration, HHS. ACTION: Notice...

76 FR 37131 - Joint Meeting of the Gastrointestinal Drugs Advisory Committee and the Drug Safety and Risk...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-06-24

... DEPARTMENT OF HEALTH AND HUMAN SERVICES Food and Drug Administration [Docket No. FDA-2011-N-0002] Joint Meeting of the Gastrointestinal Drugs Advisory Committee and the Drug Safety and Risk Management...: Gastrointestinal Drugs Advisory Committee and the Drug Safety and Risk Management Advisory Committee. General...

75 FR 12767 - Joint Meeting of the Anesthetic and Life Support Drugs Advisory Committee and the Drug Safety and...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-17

... Drugs Advisory Committee and the Drug Safety and Risk Management Advisory Committee. General Function of...] Joint Meeting of the Anesthetic and Life Support Drugs Advisory Committee and the Drug Safety and Risk Management Advisory Committee; Notice of Meeting AGENCY: Food and Drug Administration, HHS. ACTION: Notice...

75 FR 67093 - Joint Meeting of the Anesthetic and Life Support Drugs Advisory Committee and the Drug Safety and...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-11-01

...: Anesthetic and Life Support Drugs Advisory Committee and the Drug Safety and Risk Management Advisory...] Joint Meeting of the Anesthetic and Life Support Drugs Advisory Committee and the Drug Safety and Risk Management Advisory Committee; Notice of Meeting AGENCY: Food and Drug Administration, HHS. ACTION: Notice...

75 FR 32188 - Joint Meeting of the Anesthetic and Life Support Drugs Advisory Committee and the Drug Safety and...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-07

... Drugs Advisory Committee and the Drug Safety and Risk Management Advisory Committee. General Function of...] Joint Meeting of the Anesthetic and Life Support Drugs Advisory Committee and the Drug Safety and Risk Management Advisory Committee; Notice of Meeting AGENCY: Food and Drug Administration, HHS. ACTION: Notice...

75 FR 59730 - Joint Meeting of the Anesthetic and Life Support Drugs Advisory Committee and the Drug Safety and...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-28

... Drugs Advisory Committee and the Drug Safety and Risk Management Advisory Committee. General Function of...] Joint Meeting of the Anesthetic and Life Support Drugs Advisory Committee and the Drug Safety and Risk Management Advisory Committee; Notice of Meeting AGENCY: Food and Drug Administration, HHS. ACTION: Notice...

75 FR 56548 - Joint Meeting of the Peripheral and Central Nervous System Drugs Advisory Committee and the Drug...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-16

... DEPARTMENT OF HEALTH AND HUMAN SERVICES Food and Drug Administration [Docket No. FDA-2010-N-0001] Joint Meeting of the Peripheral and Central Nervous System Drugs Advisory Committee and the Drug Safety... and Central Nervous System Drugs Advisory Committee and the Drug Safety and Risk Management Advisory...

78 FR 2677 - Joint Meeting of the Advisory Committee for Reproductive Health Drugs and the Drug Safety and...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-01-14

... Reproductive Health Drugs and the Drug Safety and Risk Management Advisory Committee. General Function of the...] Joint Meeting of the Advisory Committee for Reproductive Health Drugs and the Drug Safety and Risk Management Advisory Committee; Notice of Meeting AGENCY: Food and Drug Administration, HHS. ACTION: Notice...

75 FR 10490 - Joint Meeting of the Arthritis Drugs Advisory Committee and the Drug Safety and Risk Management...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-08

... DEPARTMENT OF HEALTH AND HUMAN SERVICES Food and Drug Administration [Docket No. FDA-2010-N-0001] Joint Meeting of the Arthritis Drugs Advisory Committee and the Drug Safety and Risk Management Advisory... Drug Safety and Risk Management Advisory Committee. General Function of the Committees: To provide...

76 FR 59143 - Joint Meeting of the Advisory Committee for Reproductive Health Drugs and the Drug Safety and...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-09-23

... Reproductive Health Drugs and the Drug Safety and Risk Management Advisory Committee. General Function of the...] Joint Meeting of the Advisory Committee for Reproductive Health Drugs and the Drug Safety and Risk Management Advisory Committee; Notice of Meeting AGENCY: Food and Drug Administration, HHS. ACTION: Notice...

76 FR 59142 - Joint Meeting of the Advisory Committee for Reproductive Health Drugs and the Drug Safety and...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-09-23

... Reproductive Health Drugs and the Drug Safety and Risk Management Advisory Committee. General Function of the...] Joint Meeting of the Advisory Committee for Reproductive Health Drugs and the Drug Safety and Risk Management Advisory Committee; Notice of Meeting AGENCY: Food and Drug Administration, HHS. ACTION: Notice...

78 FR 16271 - Joint Meeting of the Advisory Committee for Reproductive Health Drugs and the Drug Safety and...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-03-14

... Reproductive Health Drugs and the Drug Safety and Risk Management Advisory Committee. General Function of the...] Joint Meeting of the Advisory Committee for Reproductive Health Drugs and the Drug Safety and Risk Management Advisory Committee; Notice of Meeting AGENCY: Food and Drug Administration, HHS. ACTION: Notice...

75 FR 55365 - Advisory Committee on Reactor Safeguards (ACRS) Meeting of the ACRS Joint Subcommittee

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-10

... NUCLEAR REGULATORY COMMISSION Advisory Committee on Reactor Safeguards (ACRS) Meeting of the ACRS Joint Subcommittee The ACRS Subcommittees on Thermal Hydraulics Phenomena; Advanced Boiling Water Reactor (ABWR); and Materials, Metallurgy, and Reactor Fuels will hold a joint meeting on October 4, 2010...

75 FR 70932 - Joint Meeting of the Anesthetic and Life Support Drugs Advisory Committee and the Drug Safety and...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-11-19

... and Risk Management Advisory Committee scheduled for December 2, 2010, is cancelled. This meeting was...] Joint Meeting of the Anesthetic and Life Support Drugs Advisory Committee and the Drug Safety and Risk Management Advisory Committee; Cancellation AGENCY: Food and Drug Administration, HHS. ACTION: Notice...

78 FR 64957 - Joint Meeting of the Gastrointestinal Drugs Advisory Committee and the Drug Safety and Risk...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-10-30

... DEPARTMENT OF HEALTH AND HUMAN SERVICES Food and Drug Administration [Docket No. FDA-2013-N-0001] Joint Meeting of the Gastrointestinal Drugs Advisory Committee and the Drug Safety and Risk Management... Committee and the Drug Safety and Risk Management Advisory Committee. General Function of the Committees: To...

76 FR 41307 - NASA Advisory Council; Space Operations Committee and Exploration Committee; Joint Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2011-07-13

... Operations Committee and Exploration Committee; Joint Meeting AGENCY: National Aeronautics and Space... the Space Operations Committee and Exploration Committee of the NASA Advisory Council. DATES: Tuesday.../Exploration Systems Mission Directorate Merger Update. [[Page 41308

75 FR 6250 - ITS Joint Program Office; Intelligent Transportation Systems Program Advisory Committee; Notice...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-02-08

... DEPARTMENT OF TRANSPORTATION ITS Joint Program Office; Intelligent Transportation Systems Program... the Intelligent Transportation Systems (ITS) Program Advisory Committee (ITSPAC). The Web conference... Transportation on all matters relating to the study, development, and implementation of intelligent...

78 FR 58543 - Advisory Board on Radiation and Worker Health (ABRWH or Advisory Board), National Institute for...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-09-24

... on Radiation and Worker Health (ABRWH or Advisory Board), National Institute for Occupational Safety... the Energy Employees Occupational Illness Compensation Program Act of 2000 to advise the President on... Subcommittee Report; SEC Issues Work Group Report on ``Sufficient Accuracy''/Co-Worker Dose Modeling; SEC...

75 FR 13643 - ITS Joint Program Office; Intelligent Transportation Systems Program Advisory Committee; Notice...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-22

... Advisory Committee; Notice of Meeting AGENCY: Research and Innovative Technology Administration, U.S... Plan; (5) Evolution of IntelliDrive\\SM\\; (6) ITS Strategic Research Plan, 2010-2014; (7) University... Technology Administration, ITS Joint Program Office, Attention: Stephen Glasscock, 1200 New Jersey Avenue, SE...

77 FR 48207 - Further Definition of “Swap,” “Security-Based Swap,” and “Security-Based Swap Agreement”; Mixed...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-13

...In accordance with section 712(a)(8), section 712(d)(1), sections 712(d)(2)(B) and (C), sections 721(b) and (c), and section 761(b) of the Dodd-Frank Wall Street Reform and Consumer Protection Act (``Dodd-Frank Act''), the Commodity Futures Trading Commission (``CFTC'') and the Securities and Exchange Commission (``SEC'') (collectively, ``Commissions''), in consultation with the Board of Governors of the Federal Reserve System (``Board''), are jointly adopting new rules and interpretations under the Commodity Exchange Act (``CEA'') and the Securities Exchange Act of 1934 (``Exchange Act'') to further define the terms ``swap,'' ``security-based swap,'' and ``security-based swap agreement'' (collectively, ``Product Definitions''); regarding ``mixed swaps;'' and governing books and records with respect to ``security-based swap agreements.'' The CFTC requests comment on its interpretation concerning forwards with embedded volumetric optionality, contained in Section II.B.2.(b)(ii) of this release.

75 FR 20895 - Establishing the President's Management Advisory Board

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-22

... Order 13538 of April 19, 2010 Establishing the President's Management Advisory Board By the authority... Administration (GSA) the President's Management Advisory Board (PMAB). Sec. 2. Mission. (a) The PMAB shall provide the President and the President's Management Council (PMC) advice and recommendations on effective...

78 FR 51266 - International Security Advisory Board (ISAB) Meeting Notice

Federal Register 2010, 2011, 2012, 2013, 2014

2013-08-20

... DEPARTMENT OF STATE [Public Notice 8419] International Security Advisory Board (ISAB) Meeting.... App Sec. 10(a)(2), the Department of State announces a meeting of the International Security Advisory... all aspects of arms control, disarmament, political-military affairs, international security and...

76 FR 23221 - Joint Public Roundtable on Issues Related to the Schedule for Implementing Final Rules for Swaps...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-26

... submissions will be reviewed jointly by the Agencies. All comments must be in English or be accompanied by an English translation. All submissions provided to either Agency in any electronic form or on paper will be... of CFTC Commissioner Scott D. O'Malia; Implementation Roundtable Seriatim; Certainty & Transparency I...

76 FR 63679 - Advisory Committee on Small and Emerging Companies

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-13

... Committee on Small and Emerging Companies AGENCY: Securities and Exchange Commission. ACTION: Notice of meeting of SEC Advisory Committee on Small and Emerging Companies. SUMMARY: The Securities and Exchange Commission Advisory Committee on Small and Emerging Companies is providing notice that it will hold a public...

77 FR 2102 - Advisory Committee on Small and Emerging Companies

Federal Register 2010, 2011, 2012, 2013, 2014

2012-01-13

... Committee on Small and Emerging Companies AGENCY: Securities and Exchange Commission. ACTION: Notice of Meeting of SEC Advisory Committee on Small and Emerging Companies. SUMMARY: The Securities and Exchange Commission Advisory Committee on Small and Emerging Companies is providing notice that it will hold a public...

75 FR 3279 - Departmental Offices; Debt Management Advisory Committee Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2010-01-20

... DEPARTMENT OF THE TREASURY Departmental Offices; Debt Management Advisory Committee Meeting Notice is hereby given, pursuant to 5 U.S.C. App. 2, Sec. 10(a)(2), of a time change for a previously... February 2, 2010. The meeting of the following debt management advisory committee: Treasury Borrowing...

77 FR 36273 - Notification of a Joint Public Meeting of the Chartered Science Advisory Board and Chartered...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-18

... Communities; Chemical Safety for Sustainability; Human Health Risk Assessment; and Homeland Security. ORD...) and a public teleconference on September 19, 2011 (76 FR 54463) to develop a report, Office of Research and Development (ORD) New Strategic Research Directions: A Joint Report of the Science Advisory...

77 FR 69869 - National Advisory Council on Alcohol Abuse and Alcoholism, National Advisory Council on Drug...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-11-21

... DEPARTMENT OF HEALTH AND HUMAN SERVICES National Institutes of Health National Advisory Council on Alcohol Abuse and Alcoholism, National Advisory Council on Drug Abuse, and National Cancer Advisory Board; Notice of Joint Meeting Pursuant to section 10(a) of the Federal Advisory Committee Act, as amended (5 U.S.C. App.), notice is hereby given o...

75 FR 22640 - Investor Advisory Committee

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-29

.... (EDT) and will be open to the public. The Committee meeting will be webcast on the Commission's Web... Committee; (iii) briefing on the Investor as Owner Subcommittee's environmental, social, and governance... statements on the Advisory Committee's Web site ( http://www.sec.gov/spotlight/investoradvisorycommittee...

75 FR 55382 - Sunshine Act Meetings

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-10

... of Public Affairs at (202) 418-5080 or the SEC's office of Public Affairs at (202) 551-4120. Dated... provisions of the Government in the Sunshine Act, Public Law 94-409, that the Securities and Exchange Commission (``SEC'') and the Commodity Futures Trading Commission (``CFTC'') will hold public roundtable...

78 FR 41832 - Departmental Offices; Debt Management Advisory Committee Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2013-07-11

... DEPARTMENT OF THE TREASURY Departmental Offices; Debt Management Advisory Committee Meeting Notice is hereby given, pursuant to 5 U.S.C. App. 2, Sec. 10(a)(2), that a meeting will be held at the Hay-Adams Hotel, 16th Street and Pennsylvania Avenue NW., Washington, DC, on July 30, 2013 at 9:30 a.m. of the following debt management advisory...

76 FR 22938 - Sunshine Act Meetings

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-25

... of Public Affairs at (202) 418-5080 or the SEC's office of Public Affairs at (202) 551-4120. Dated... provisions of the Government in the Sunshine Act, Public Law 94-409, that the Securities and Exchange Commission (``SEC'') and the Commodity Futures Trading Commission (``CFTC'') will hold a public roundtable to...

Efficient coupling of Sec23-Sec24 to Sec13-Sec31 drives COPII-dependent collagen secretion and is essential for normal craniofacial development.

PubMed

Townley, Anna K; Feng, Yi; Schmidt, Katy; Carter, Deborah A; Porter, Robert; Verkade, Paul; Stephens, David J

2008-09-15

The COPII coat assembles on endoplasmic reticulum membranes to coordinate the collection of secretory cargo with the formation of transport vesicles. During COPII assembly, Sar1 deforms the membrane and recruits the Sec23-Sec24 complex (Sec23/24), which is the primary cargo-binding adaptor for the system, and Sec13-Sec31 (Sec13/31), which provides a structural outer layer for vesicle formation. Here we show that Sec13 depletion results in concomitant loss of Sec31 and juxtanuclear clustering of pre-budding complexes containing Sec23/24 and cargo. Electron microscopy reveals the presence of curved coated profiles on distended endoplasmic reticulum, indicating that Sec13/31 is not required for the generation or maintenance of the curvature. Surprisingly, export of tsO45-G-YFP, a marker of secretory cargo, is unaffected by Sec13/31 depletion; by contrast, secretion of collagen from primary fibroblasts is strongly inhibited. Suppression of Sec13 expression in zebrafish causes defects in proteoglycan deposition and skeletal abnormalities that are grossly similar to the craniofacial abnormalities of crusher mutant zebrafish and patients with cranio-lenticulo-sutural dysplasia. We conclude that efficient coupling of the inner (Sec23/24) and outer (Sec13/31) layers of the COPII coat is required to drive the export of collagen from the endoplasmic reticulum, and that highly efficient COPII assembly is essential for normal craniofacial development during embryogenesis.

Structural Similarities and Differences between Two Functionally Distinct SecA Proteins, Mycobacterium tuberculosis SecA1 and SecA2

PubMed Central

Swanson, Stephanie; Ioerger, Thomas R.; Rigel, Nathan W.; Miller, Brittany K.; Braunstein, Miriam

2015-01-01

ABSTRACT While SecA is the ATPase component of the major bacterial secretory (Sec) system, mycobacteria and some Gram-positive pathogens have a second paralog, SecA2. In bacteria with two SecA paralogs, each SecA is functionally distinct, and they cannot compensate for one another. Compared to SecA1, SecA2 exports a distinct and smaller set of substrates, some of which have roles in virulence. In the mycobacterial system, some SecA2-dependent substrates lack a signal peptide, while others contain a signal peptide but possess features in the mature protein that necessitate a role for SecA2 in their export. It is unclear how SecA2 functions in protein export, and one open question is whether SecA2 works with the canonical SecYEG channel to export proteins. In this study, we report the structure of Mycobacterium tuberculosis SecA2 (MtbSecA2), which is the first structure of any SecA2 protein. A high level of structural similarity is observed between SecA2 and SecA1. The major structural difference is the absence of the helical wing domain, which is likely to play a role in how MtbSecA2 recognizes its unique substrates. Importantly, structural features critical to the interaction between SecA1 and SecYEG are preserved in SecA2. Furthermore, suppressor mutations of a dominant-negative secA2 mutant map to the surface of SecA2 and help identify functional regions of SecA2 that may promote interactions with SecYEG or the translocating polypeptide substrate. These results support a model in which the mycobacterial SecA2 works with SecYEG. IMPORTANCE SecA2 is a paralog of SecA1, which is the ATPase of the canonical bacterial Sec secretion system. SecA2 has a nonredundant function with SecA1, and SecA2 exports a distinct and smaller set of substrates than SecA1. This work reports the crystal structure of SecA2 of Mycobacterium tuberculosis (the first SecA2 structure reported for any organism). Many of the structural features of SecA1 are conserved in the SecA2 structure

75 FR 22561 - Federal Advisory Committee; United States Strategic Command Strategic Advisory Group; Charter...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-29

... Command Strategic Advisory Group; Charter Renewal AGENCY: Department of Defense (DoD). ACTION: Renewal of... Command Strategic Advisory Group (hereafter referred to as the Group). FOR FURTHER INFORMATION CONTACT... Chairman of the Joint Chiefs of Staff and the Commander of the U.S. Strategic Command independent advice...

46 CFR Sec. 6 - Disbursements at other domestic ports.

Code of Federal Regulations, 2010 CFR

2010-10-01

... invoices from such advances and make proper accounting to the agent for each advance supported by invoices... RULES FOR FINANCIAL TRANSACTIONS UNDER AGENCY AGREEMENTS Disbursements Sec. 6 Disbursements at other... agent may advance from time to time from the joint bank account the funds necessary to meet the...

78 FR 57174 - Advisory Committee Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2013-09-17

... recommended for inclusion on future Joint Board examinations in actuarial mathematics, pension law and... Advisory Committee on Actuarial Examinations. DATES: The meeting will be held on October 18, 2013, from 8.... SUPPLEMENTARY INFORMATION: Notice is hereby given that the Advisory Committee on Actuarial Examinations will...

78 FR 29122 - Strategic Environmental Research and Development Program, Scientific Advisory Board; Notice of...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-05-17

... Program, Scientific Advisory Board (SAB). This notice is published in accordance with Section 10(a)(2) of the Federal Advisory Committee Act (Pub. L. 92-463). DATES: Thursday, June 13, 2013 from 9:00 a.m. to..., Subtitle A, Part IV, Chapter 172, Sec. 2904. The full agenda follows: 9:00 a.m. Convene Mr. Joseph Francis...

77 FR 63337 - Renewal of Charter of Advisory Committee on Actuarial Examinations

Federal Register 2010, 2011, 2012, 2013, 2014

2012-10-16

... examinations in actuarial mathematics and methodology. The Joint Board administers such examinations in... JOINT BOARD FOR THE ENROLLMENT OF ACTUARIES Renewal of Charter of Advisory Committee on Actuarial... the Advisory Committee on Actuarial Examinations. FOR FURTHER INFORMATION CONTACT: Patrick McDonough...

Joint Chiefs of Staff > Directorates > J7 | Joint Force Development

Science.gov Websites

development: Doctrine, Education, Concept Development & Experimentation, Training, Exercises and Lessons Coalition Partners. Joint Education Develop policies governing officer and enlisted Joint Professional Military Education (JPME), and the National Defense University. Direct JPME educational advisory group and

76 FR 36930 - National Advisory Council on Alcohol Abuse and Alcoholism and National Advisory Council on Drug...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-06-23

... National Institute on Drug Abuse. The meeting will be open to the public as indicated below, with... Alcohol Abuse and Alcoholism and National Advisory Council on Drug Abuse; Notice of Joint Meeting Pursuant... Alcoholism and National Advisory Council on Drug Abuse. Date: September 12, 2011. Open: September 12, 2011...

22 CFR 401.28 - Advisory boards.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 22 Foreign Relations 2 2010-04-01 2010-04-01 true Advisory boards. 401.28 Section 401.28 Foreign Relations INTERNATIONAL JOINT COMMISSION, UNITED STATES AND CANADA RULES OF PROCEDURE References § 401.28 Advisory boards. (a) The Commission may appoint a board or boards, composed of qualified persons, to...

Regulation of exocytosis by the exocyst subunit Sec6 and the SM protein Sec1.

PubMed

Morgera, Francesca; Sallah, Margaret R; Dubuke, Michelle L; Gandhi, Pallavi; Brewer, Daniel N; Carr, Chavela M; Munson, Mary

2012-01-01

Trafficking of protein and lipid cargo through the secretory pathway in eukaryotic cells is mediated by membrane-bound vesicles. Secretory vesicle targeting and fusion require a conserved multisubunit protein complex termed the exocyst, which has been implicated in specific tethering of vesicles to sites of polarized exocytosis. The exocyst is directly involved in regulating soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein receptor (SNARE) complexes and membrane fusion through interactions between the Sec6 subunit and the plasma membrane SNARE protein Sec9. Here we show another facet of Sec6 function-it directly binds Sec1, another SNARE regulator, but of the Sec1/Munc18 family. The Sec6-Sec1 interaction is exclusive of Sec6-Sec9 but compatible with Sec6-exocyst assembly. In contrast, the Sec6-exocyst interaction is incompatible with Sec6-Sec9. Therefore, upon vesicle arrival, Sec6 is proposed to release Sec9 in favor of Sec6-exocyst assembly and to simultaneously recruit Sec1 to sites of secretion for coordinated SNARE complex formation and membrane fusion.

76 FR 75912 - Meeting of the Advisory Committee

Federal Register 2010, 2011, 2012, 2013, 2014

2011-12-05

... Joint Board examinations in actuarial mathematics and methodology referred to in 29 U.S.C. 1242(a)(1)(B... Advisory Committee on Actuarial Examinations (portions of which will be open to the public) in Washington.... SUPPLEMENTARY INFORMATION: Notice is hereby given that the Advisory Committee on Actuarial Examinations will...

78 FR 73564 - Meeting of the Advisory Committee

Federal Register 2010, 2011, 2012, 2013, 2014

2013-12-06

... Joint Board examinations in actuarial mathematics and methodology referred to in 29 U.S.C. 1242(a)(1)(B... Advisory Committee on Actuarial Examinations (portions of which will be open to the public) in Washington.... SUPPLEMENTARY INFORMATION: Notice is hereby given that the Advisory Committee on Actuarial Examinations will...

Nonlethal sec71-1 and sec72-1 mutations eliminate proteins associated with the Sec63p-BiP complex from S. cerevisiae.

PubMed Central

Fang, H; Green, N

1994-01-01

The sec71-1 and sec72-1 mutations were identified by a genetic assay that monitored membrane protein integration into the endoplasmic reticulum (ER) membrane of the yeast Saccharomyces cerevisiae. The mutations inhibited integration of various chimeric membrane proteins and translocation of a subset of water soluble proteins. In this paper we show that SEC71 encodes the 31.5-kDa transmembrane glycoprotein (p31.5) and SEC72 encodes the 23-kDa protein (p23) of the Sec63p-BiP complex. SEC71 is therefore identical to SEC66 (HSS1), which was previously shown to encode p31.5. DNA sequence analyses reveal that sec71-1 cells contain a nonsense mutation that removes approximately two-thirds of the cytoplasmic C-terminal domain of p31.5. The sec72-1 mutation shifts the reading frame of the gene encoding p23. Unexpectedly, the sec71-1 mutant lacks p31.5 and p23. Neither mutation is lethal, although sec71-1 cells exhibit a growth defect at 37 degrees C. These results show that p31.5 and p23 are important for the trafficking of a subset of proteins to the ER membrane. Images PMID:7841522

78 FR 19008 - Meeting of the Advisory Committee; Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2013-03-28

... inclusion on future Joint Board examinations in actuarial mathematics, pension law and methodology referred... closed meeting of the Advisory Committee on Actuarial Examinations. DATES: The meeting will be held on.... SUPPLEMENTARY INFORMATION: Notice is hereby given that the Advisory Committee on Actuarial Examinations will...

75 FR 16510 - Meeting of the Advisory Committee; Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-01

... recommended for inclusion on future Joint Board examinations in actuarial mathematics, pension law and... closed meeting of the Advisory Committee on Actuarial Examinations. DATES: The meeting will be held on.... SUPPLEMENTARY INFORMATION: Notice is hereby given that the Advisory Committee on Actuarial Examinations will...

75 FR 59292 - Meeting of the Advisory Committee; Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-27

... inclusion on future Joint Board examinations in actuarial mathematics, pension law and methodology referred... closed meeting of the Advisory Committee on Actuarial Examinations. DATES: The meeting will be held on... INFORMATION: Notice is hereby given that the Advisory Committee on Actuarial Examinations will meet at...

75 FR 3488 - Flight 93 National Memorial Advisory Commission

Federal Register 2010, 2011, 2012, 2013, 2014

2010-01-21

... DEPARTMENT OF THE INTERIOR National Park Service Flight 93 National Memorial Advisory Commission... forth the date of the February 6, 2010 meeting of the Flight 93 Advisory Commission. DATES: The public.... (Eastern). The Commission will meet jointly with the Flight 93 Memorial Task Force. Location: The meeting...

75 FR 43199 - Flight 93 National Memorial Advisory Commission

Federal Register 2010, 2011, 2012, 2013, 2014

2010-07-23

... DEPARTMENT OF THE INTERIOR National Park Service Flight 93 National Memorial Advisory Commission... sets forth the date of the August 7, 2010, meeting of the Flight 93 Advisory Commission. DATES: The.... (Eastern). The Commission will meet jointly with the Flight 93 Memorial Task Force. Location: The meeting...

75 FR 76486 - Meeting of the Advisory Committee; Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2010-12-08

... that may be recommended for inclusion on future Joint Board examinations in actuarial mathematics and... meeting of the Advisory Committee on Actuarial Examinations (portions of which will be open to the public... the Advisory Committee on Actuarial Examinations will meet at the Internal Revenue Service, 1111...

76 FR 4131 - Flight 93 National Memorial Advisory Commission

Federal Register 2010, 2011, 2012, 2013, 2014

2011-01-24

... DEPARTMENT OF THE INTERIOR National Park Service Flight 93 National Memorial Advisory Commission... notice sets forth the date of the February 5, 2011, meeting of the Flight 93 Advisory Commission. DATES.... to 1 p.m. (Eastern). The Commission will meet jointly with the Flight 93 Memorial Task Force...

75 FR 17158 - Flight 93 National Memorial Advisory Commission

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-05

... DEPARTMENT OF THE INTERIOR National Park Service Flight 93 National Memorial Advisory Commission... sets forth the date of the May 1, 2010, meeting of the Flight 93 Advisory Commission. DATES: The public...). The Commission will meet jointly with the Flight 93 Memorial Task Force. Location: The meeting will be...

77 FR 19034 - Meeting of the Advisory Committee; Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-29

... Joint Board examinations in actuarial mathematics, pension law and methodology referred to in 29 U.S.C... closed meeting of the Advisory Committee on Actuarial Examinations. DATES: The meeting will be held on... INFORMATION: Notice is hereby given that the Advisory Committee on Actuarial Examinations will meet at...

75 FR 28062 - Meeting of the Advisory Committee; Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2010-05-19

... questions which may be recommended for inclusion on future Joint Board examinations in actuarial mathematics... meeting of the Advisory Committee on Actuarial Examinations (a portion of which will be open to the public... the Advisory Committee on Actuarial Examinations will meet in at the Internal Revenue Service Building...

76 FR 17967 - Meeting of the Advisory Committee; Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2011-03-31

... Joint Board examinations in actuarial mathematics, pension law and methodology referred to in 29 U.S.C... closed meeting of the Advisory Committee on Actuarial Examinations. DATES: The meeting will be held on... INFORMATION: Notice is hereby given that the Advisory Committee on Actuarial Examinations will meet at Mercer...

75 FR 50718 - Acknowledgment Letters for Customer Funds and Secured Amount Funds; Correction

Federal Register 2010, 2011, 2012, 2013, 2014

2010-08-17

... RIN 3038-AC72 Acknowledgment Letters for Customer Funds and Secured Amount Funds; Correction AGENCY... August 9, 2010, regarding Acknowledgment Letters for Customer Funds and Secured Amount Funds. FOR FURTHER... for CFTC Regulation 1.20 Customer Segregated Account'' to read ``Appendix A to Sec. 1.20...

77 FR 18814 - Emergency Access Advisory Committee; Announcement of Date of Next Meeting

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2012-03-28

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78 FR 36575 - Meeting of the Advisory Committee; Meeting

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2013-06-18

... Joint Board examinations in actuarial mathematics and methodology referred to in 29 U.S.C. 1242(a)(1)(B... meeting of the Advisory Committee on Actuarial Examinations (a portion of which will be open to the public...- 2173. SUPPLEMENTARY INFORMATION: Notice is hereby given that the Advisory Committee on Actuarial...

77 FR 51845 - Intelligent Transportation Systems Program Advisory Committee; Notice of Meeting

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2012-08-27

... DEPARTMENT OF TRANSPORTATION Intelligent Transportation Systems Program Advisory Committee; Notice.... Department of Transportation. ACTION: Notice. The Intelligent Transportation Systems (ITS) Program Advisory..., development, and implementation of intelligent transportation systems. Through its sponsor, the ITS Joint...

77 FR 27825 - Self-Regulatory Organizations; ICE Clear Europe Limited; Notice of Filing and Order Granting...

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2012-05-11

...-Regulatory Organizations; ICE Clear Europe Limited; Notice of Filing and Order Granting Accelerated Approval...'') \\1\\ and Rule 19b-4 thereunder,\\2\\ notice is hereby given that on May 5, 2012, ICE Clear Europe... such as the FSA, CFTC, SEC, Banque de France, Bundesbank, Bundesanstalt f[uuml]r...

CFTC/CFDT attitudes towards immigration in the Parisian region: making immigrant workers' condition a cause.

PubMed

Blévis, Laure; Pezet, Eric

2012-01-01

This paper, which is based on the detailed analysis of the post-war archives of the French Christian union Confédération Française des Travailleurs Chrétiens (CFTC), which became the Confédération Française Démocratique du Travail (CFDT) in 1964, highlights the difficulties, both from a practical and ideological point of view, for a militant organisation to embrace the cause of immigrant workers and to give them a voice. The CFDT had to ‘construct’ immigrant workers as a group they could represent, which means as a group that relates to French workers, despite possible xenophobia. A key moment was the denunciation of their housing conditions that make immigrant workers not competitors in the job market but victims of injustice. The union had to reinvent its engagement frames in order to include the specific problems faced by the immigrant workforce. The study shows that the urban dimension was essential in this process as Paris slums made visible an important plight of migrants and provided the opportunity to change public opinion.

78 FR 16341 - Self-Regulatory Organizations; Financial Industry Regulatory Authority, Inc.; Notice of Filing...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-03-14

... regulatory regime applicable to the over-the-counter derivatives markets. Title VII provides the SEC and the CFTC with tools to oversee these markets.\\4\\ Under the comprehensive framework established in Title VII... Rule),\\11\\ arising from the daily mark to market of the CDS (``variation margin''). FINRA notes that...

76 FR 45308 - Sunshine Act Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2011-07-28

.... For further information, please contact the CFTC's Office of Public Affairs at (202) 418-5080 or the SEC's Office of Public Affairs at (202) 551-4120. Dated: July 25, 2011. Elizabeth M. Murphy, Secretary... provisions of the Government in the Sunshine Act, Public Law 94-409, that the Securities and Exchange...

75 FR 63505 - Renewal of Advisory Committee on Actuarial Examinations

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-15

... actuarial mathematics and methodology. The Joint Board administers such examinations in discharging its... JOINT BOARD FOR THE ENROLLMENT OF ACTUARIES Renewal of Advisory Committee on Actuarial... Committee on Actuarial Examinations. FOR FURTHER INFORMATION CONTACT: Patrick W. McDonough, 202-622-8225...

Suppressor Analysis Reveals a Role for SecY in the SecA2-Dependent Protein Export Pathway of Mycobacteria

PubMed Central

Ligon, Lauren S.; Rigel, Nathan W.; Romanchuk, Artur; Jones, Corbin D.

2013-01-01

All bacteria use the conserved Sec pathway to transport proteins across the cytoplasmic membrane, with the SecA ATPase playing a central role in the process. Mycobacteria are part of a small group of bacteria that have two SecA proteins: the canonical SecA (SecA1) and a second, specialized SecA (SecA2). The SecA2-dependent pathway exports a small subset of proteins and is required for Mycobacterium tuberculosis virulence. The mechanism by which SecA2 drives export of proteins across the cytoplasmic membrane remains poorly understood. Here we performed suppressor analysis on a dominant negative secA2 mutant (secA2 K129R) of the model mycobacterium Mycobacterium smegmatis to better understand the pathway used by SecA2 to export proteins. Two extragenic suppressor mutations were identified as mapping to the promoter region of secY, which encodes the central component of the canonical Sec export channel. These suppressor mutations increased secY expression, and this effect was sufficient to alleviate the secA2 K129R phenotype. We also discovered that the level of SecY protein was greatly diminished in the secA2 K129R mutant, but at least partially restored in the suppressors. Furthermore, the level of SecY in a suppressor strongly correlated with the degree of suppression. Our findings reveal a detrimental effect of SecA2 K129R on SecY, arguing for an integrated system in which SecA2 works with SecY and the canonical Sec translocase to export proteins. PMID:23913320

Two alternative binding mechanisms connect the protein translocation Sec71-Sec72 complex with heat shock proteins.

PubMed

Tripathi, Arati; Mandon, Elisabet C; Gilmore, Reid; Rapoport, Tom A

2017-05-12

The biosynthesis of many eukaryotic proteins requires accurate targeting to and translocation across the endoplasmic reticulum membrane. Post-translational protein translocation in yeast requires both the Sec61 translocation channel, and a complex of four additional proteins: Sec63, Sec62, Sec71, and Sec72. The structure and function of these proteins are largely unknown. This pathway also requires the cytosolic Hsp70 protein Ssa1, but whether Ssa1 associates with the translocation machinery to target protein substrates to the membrane is unclear. Here, we use a combined structural and biochemical approach to explore the role of Sec71-Sec72 subcomplex in post-translational protein translocation. To this end, we report a crystal structure of the Sec71-Sec72 complex, which revealed that Sec72 contains a tetratricopeptide repeat (TPR) domain that is anchored to the endoplasmic reticulum membrane by Sec71. We also determined the crystal structure of this TPR domain with a C-terminal peptide derived from Ssa1, which suggests how Sec72 interacts with full-length Ssa1. Surprisingly, Ssb1, a cytoplasmic Hsp70 that binds ribosome-associated nascent polypeptide chains, also binds to the TPR domain of Sec72, even though it lacks the TPR-binding C-terminal residues of Ssa1. We demonstrate that Ssb1 binds through its ATPase domain to the TPR domain, an interaction that leads to inhibition of nucleotide exchange. Taken together, our results suggest that translocation substrates can be recruited to the Sec71-Sec72 complex either post-translationally through Ssa1 or co-translationally through Ssb1. © 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

Two alternative binding mechanisms connect the protein translocation Sec71-Sec72 complex with heat shock proteins

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tripathi, Arati; Mandon, Elisabet C.; Gilmore, Reid

The biosynthesis of many eukaryotic proteins requires accurate targeting to and translocation across the endoplasmic reticulum membrane. Post-translational protein translocation in yeast requires both the Sec61 translocation channel, and a complex of four additional proteins: Sec63, Sec62, Sec71, and Sec72. The structure and function of these proteins are largely unknown. This pathway also requires the cytosolic Hsp70 protein Ssa1, but whether Ssa1 associates with the translocation machinery to target protein substrates to the membrane is unclear. Here, we use a combined structural and biochemical approach to explore the role of Sec71-Sec72 subcomplex in post-translational protein translocation. To this end, wemore » report a crystal structure of the Sec71-Sec72 complex, which revealed that Sec72 contains a tetratricopeptide repeat (TPR) domain that is anchored to the endoplasmic reticulum membrane by Sec71. We also determined the crystal structure of this TPR domain with a C-terminal peptide derived from Ssa1, which suggests how Sec72 interacts with full-length Ssa1. Surprisingly, Ssb1, a cytoplasmic Hsp70 that binds ribosome-associated nascent polypeptide chains, also binds to the TPR domain of Sec72, even though it lacks the TPR-binding C-terminal residues of Ssa1. We demonstrate that Ssb1 binds through its ATPase domain to the TPR domain, an interaction that leads to inhibition of nucleotide exchange. Taken together, our results suggest that translocation substrates can be recruited to the Sec71-Sec72 complex either post-translationally through Ssa1 or co-translationally through Ssb1.« less

75 FR 41240 - NASA Advisory Council; Technology and Innovation Committee; Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2010-07-15

... and Innovation Committee; Meeting AGENCY: National Aeronautics and Space Administration. ACTION: N... Innovation Committee of the NASA Advisory Council. It will include a joint session with the Exploration... Open Collaboration and Innovation Presentation Update on Human Exploration Framework Team (HEFT) (joint...

In Candida albicans hyphae, Sec2p is physically associated with SEC2 mRNA on secretory vesicles.

PubMed

Caballero-Lima, David; Hautbergue, Guillaume M; Wilson, Stuart A; Sudbery, Peter E

2014-11-01

Candida albicans hyphae grow in a highly polarized fashion from their tips. This polarized growth requires the continuous delivery of secretory vesicles to the tip region. Vesicle delivery depends on Sec2p, the Guanine Exchange Factor (GEF) for the Rab GTPase Sec4p. GTP bound Sec4p is required for the transit of secretory vesicles from the trans-Golgi to sites of polarized growth. We previously showed that phosphorylation of Sec2p at residue S584 was necessary for Sec2p to support hyphal, but not yeast growth. Here we show that on secretory vesicles SEC2 mRNA is physically associated with Sec2p. Moreover, we show that the phosphorylation of S584 allows SEC2 mRNA to dissociate from Sec2p and we speculate that this is necessary for Sec2p function and/or translation. During hyphal extension, the growing tip may be separated from the nucleus by up to 15 μm. Transport of SEC2 mRNA on secretory vesicles to the tip localizes SEC2 translation to tip allowing a sufficient accumulation of this key protein at the site of polarized growth. © 2014 The Authors. Molecular Microbiology published by John Wiley & Sons Ltd.

Two-way communication between SecY and SecA suggests a Brownian ratchet mechanism for protein translocation.

PubMed

Allen, William John; Corey, Robin Adam; Oatley, Peter; Sessions, Richard Barry; Baldwin, Steve A; Radford, Sheena E; Tuma, Roman; Collinson, Ian

2016-05-16

The essential process of protein secretion is achieved by the ubiquitous Sec machinery. In prokaryotes, the drive for translocation comes from ATP hydrolysis by the cytosolic motor-protein SecA, in concert with the proton motive force (PMF). However, the mechanism through which ATP hydrolysis by SecA is coupled to directional movement through SecYEG is unclear. Here, we combine all-atom molecular dynamics (MD) simulations with single molecule FRET and biochemical assays. We show that ATP binding by SecA causes opening of the SecY-channel at long range, while substrates at the SecY-channel entrance feed back to regulate nucleotide exchange by SecA. This two-way communication suggests a new, unifying 'Brownian ratchet' mechanism, whereby ATP binding and hydrolysis bias the direction of polypeptide diffusion. The model represents a solution to the problem of transporting inherently variable substrates such as polypeptides, and may underlie mechanisms of other motors that translocate proteins and nucleic acids.

75 FR 40852 - NASA Advisory Council; Exploration Committee; Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2010-07-14

... Cooperation and Other Partnerships. Joint Session with NASA Advisory Council Technology & Innovation Committee... expiration date, U.S. Social Security Number (if applicable), Permanent Resident Alien card number and...

76 FR 62817 - National Offshore Safety Advisory Committee

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-11

... Joint Investigation Team for DEEPWATER HORIZON drilling rig explosion and sinking. (9) Update from the... Advisory Committee>NOSAC and then use the event key. The meeting will be recorded by a court reporter. A...

Pancreatic SEC23B deficiency is sufficient to explain the perinatal lethality of germline SEC23B deficiency in mice

PubMed Central

Khoriaty, Rami; Everett, Lesley; Chase, Jennifer; Zhu, Guojing; Hoenerhoff, Mark; McKnight, Brooke; Vasievich, Matthew P.; Zhang, Bin; Tomberg, Kärt; Williams, John; Maillard, Ivan; Ginsburg, David

2016-01-01

In humans, loss of function mutations in SEC23B result in Congenital Dyserythropoietic Anemia type II (CDAII), a disease limited to defective erythroid development. Patients with two nonsense SEC23B mutations have not been reported, suggesting that complete SEC23B deficiency might be lethal. We previously reported that SEC23B-deficient mice die perinatally, exhibiting massive pancreatic degeneration and that mice with hematopoietic SEC23B deficiency do not exhibit CDAII. We now show that SEC23B deficiency restricted to the pancreas is sufficient to explain the lethality observed in mice with global SEC23B-deficiency. Immunohistochemical stains demonstrate an acinar cell defect but normal islet cells. Mammalian genomes contain two Sec23 paralogs, Sec23A and Sec23B. The encoded proteins share ~85% amino acid sequence identity. We generate mice with pancreatic SEC23A deficiency and demonstrate that these mice survive normally, exhibiting normal pancreatic weights and histology. Taken together, these data demonstrate that SEC23B but not SEC23A is essential for murine pancreatic development. We also demonstrate that two BAC transgenes spanning Sec23b rescue the lethality of mice homozygous for a Sec23b gene trap allele, excluding a passenger gene mutation as the cause of the pancreatic lethality, and indicating that the regulatory elements critical for Sec23b pancreatic function reside within the BAC transgenes. PMID:27297878

Joint attention studies in normal and autistic children using NIRS

NASA Astrophysics Data System (ADS)

Chaudhary, Ujwal; Hall, Michael; Gutierrez, Anibal; Messinger, Daniel; Rey, Gustavo; Godavarty, Anuradha

2011-03-01

Autism is a socio-communication brain development disorder. It is marked by degeneration in the ability to respond to joint attention skill task, from as early as 12 to 18 months of age. This trait is used to distinguish autistic from nonautistic. In this study Near infrared spectroscopy (NIRS) is being applied for the first time to study the difference in activation and connectivity in the frontal cortex of typically developing (TD) and autistic children between 4-8 years of age in response to joint attention task. The optical measurements are acquired in real time from frontal cortex using Imagent (ISS Inc.) - a frequency domain based NIRS system in response to video clips which engenders a feeling of joint attention experience in the subjects. A block design consisting of 5 blocks of following sequence 30 sec joint attention clip (J), 30 sec non-joint attention clip (NJ) and 30 sec rest condition is used. Preliminary results from TD child shows difference in brain activation (in terms of oxy-hemoglobin, HbO) during joint attention interaction compared to the nonjoint interaction and rest. Similar activation study did not reveal significant differences in HbO across the stimuli in, unlike in an autistic child. Extensive studies are carried out to validate the initial observations from both brain activation as well as connectivity analysis. The result has significant implication for research in neural pathways associated with autism that can be mapped using NIRS.

Epithelial organization and cyst lumen expansion require efficient Sec13-Sec31-driven secretion.

PubMed

Townley, Anna K; Schmidt, Katy; Hodgson, Lorna; Stephens, David J

2012-02-01

Epithelial morphogenesis is directed by interactions with the underlying extracellular matrix. Secretion of collagen and other matrix components requires efficient coat complex II (COPII) vesicle formation at the endoplasmic reticulum. Here, we show that suppression of the outer layer COPII component, Sec13, in zebrafish embryos results in a disorganized gut epithelium. In human intestinal epithelial cells (Caco-2), Sec13 depletion causes defective epithelial polarity and organization on permeable supports. Defects are seen in the ability of cells to adhere to the substrate, form a monolayer and form intercellular junctions. When embedded in a three-dimensional matrix, Sec13-depleted Caco-2 cells form cysts but, unlike controls, are defective in lumen expansion. Incorporation of primary fibroblasts within the three-dimensional culture substantially restores normal morphogenesis. We conclude that efficient COPII-dependent secretion, notably assembly of Sec13-Sec31, is required to drive epithelial morphogenesis in both two- and three-dimensional cultures in vitro, as well as in vivo. Our results provide insight into the role of COPII in epithelial morphogenesis and have implications for the interpretation of epithelial polarity and organization assays in cell culture.

Direct identification of the site of binding on the chaperone SecB for the amino terminus of the translocon motor SecA.

PubMed

Randall, Linda L; Henzl, Michael T

2010-06-01

Protein export mediated by the general secretory Sec system in Escherichia coli proceeds by a dynamic transfer of a precursor polypeptide from the chaperone SecB to the SecA ATPase motor of the translocon and subsequently into and through the channel of the membrane-embedded SecYEG heterotrimer. The complex between SecA and SecB is stabilized by several separate sites of contact. Here we have demonstrated directly an interaction between the N-terminal residues 2 through 11 of SecA and the C-terminal 13 residues of SecB by isothermal titration calorimetry and analytical sedimentation velocity centrifugation. We discuss the unusual binding properties of SecA and SecB in context of a model for transfer of the precursor along the pathway of export.

Epithelial organization and cyst lumen expansion require efficient Sec13–Sec31-driven secretion

PubMed Central

Townley, Anna K.; Schmidt, Katy; Hodgson, Lorna; Stephens, David J.

2012-01-01

Epithelial morphogenesis is directed by interactions with the underlying extracellular matrix. Secretion of collagen and other matrix components requires efficient coat complex II (COPII) vesicle formation at the endoplasmic reticulum. Here, we show that suppression of the outer layer COPII component, Sec13, in zebrafish embryos results in a disorganized gut epithelium. In human intestinal epithelial cells (Caco-2), Sec13 depletion causes defective epithelial polarity and organization on permeable supports. Defects are seen in the ability of cells to adhere to the substrate, form a monolayer and form intercellular junctions. When embedded in a three-dimensional matrix, Sec13-depleted Caco-2 cells form cysts but, unlike controls, are defective in lumen expansion. Incorporation of primary fibroblasts within the three-dimensional culture substantially restores normal morphogenesis. We conclude that efficient COPII-dependent secretion, notably assembly of Sec13–Sec31, is required to drive epithelial morphogenesis in both two- and three-dimensional cultures in vitro, as well as in vivo. Our results provide insight into the role of COPII in epithelial morphogenesis and have implications for the interpretation of epithelial polarity and organization assays in cell culture. PMID:22331354

46 CFR Sec. 3 - Specifications.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 8 2011-10-01 2011-10-01 false Specifications. Sec. 3 Section 3 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION A-NATIONAL SHIPPING AUTHORITY PROCEDURE FOR ACCOMPLISHMENT OF VESSEL REPAIRS UNDER NATIONAL SHIPPING AUTHORITY MASTER LUMP SUM REPAIR CONTRACT-NSA-LUMPSUMREP Sec. 3...

46 CFR Sec. 3 - Specifications.

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2013-10-01

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46 CFR Sec. 9 - Payment.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 8 2011-10-01 2011-10-01 false Payment. Sec. 9 Section 9 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION A-NATIONAL SHIPPING AUTHORITY PROCEDURE FOR ACCOMPLISHMENT OF VESSEL REPAIRS UNDER NATIONAL SHIPPING AUTHORITY MASTER LUMP SUM REPAIR CONTRACT-NSA-LUMPSUMREP Sec. 9 Payment...

46 CFR Sec. 3 - Specifications.

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46 CFR Sec. 9 - Payment.

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2012-10-01

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46 CFR Sec. 9 - Payment.

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46 CFR Sec. 9 - Payment.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 8 2014-10-01 2014-10-01 false Payment. Sec. 9 Section 9 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION A-NATIONAL SHIPPING AUTHORITY PROCEDURE FOR ACCOMPLISHMENT OF VESSEL REPAIRS UNDER NATIONAL SHIPPING AUTHORITY MASTER LUMP SUM REPAIR CONTRACT-NSA-LUMPSUMREP Sec. 9 Payment...

46 CFR Sec. 9 - Payment.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 8 2010-10-01 2010-10-01 false Payment. Sec. 9 Section 9 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION A-NATIONAL SHIPPING AUTHORITY PROCEDURE FOR ACCOMPLISHMENT OF VESSEL REPAIRS UNDER NATIONAL SHIPPING AUTHORITY MASTER LUMP SUM REPAIR CONTRACT-NSA-LUMPSUMREP Sec. 9 Payment...

46 CFR Sec. 3 - Specifications.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 8 2010-10-01 2010-10-01 false Specifications. Sec. 3 Section 3 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION A-NATIONAL SHIPPING AUTHORITY PROCEDURE FOR ACCOMPLISHMENT OF VESSEL REPAIRS UNDER NATIONAL SHIPPING AUTHORITY MASTER LUMP SUM REPAIR CONTRACT-NSA-LUMPSUMREP Sec. 3...

46 CFR Sec. 3 - Specifications.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 8 2012-10-01 2012-10-01 false Specifications. Sec. 3 Section 3 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION A-NATIONAL SHIPPING AUTHORITY PROCEDURE FOR ACCOMPLISHMENT OF VESSEL REPAIRS UNDER NATIONAL SHIPPING AUTHORITY MASTER LUMP SUM REPAIR CONTRACT-NSA-LUMPSUMREP Sec. 3...

Travel Health Advisory Group: a joint travel industry and travel health Special Interest Group promoting healthy travel in Australia.

PubMed

Leggat, Peter A; Zwar, Nicholas; Hudson, Bernie

2012-09-01

The Travel Health Advisory Group (THAG), established in 1997, is a joint initiative between the travel industry and travel health professionals in Australia that aims to promote healthy travel. THAG seeks to promote cooperation in improving the health of travellers between the travel industry and travel medicine professionals and to raise public awareness of the importance of travel health. From 2011, THAG has been a Special Interest Group of The Australasian College of Tropical Medicine and its membership has been active in several areas, including web-based travel health information, travel health promotion, media releases, research and education in Australia. Information is given on the objectives, membership and an overview of the various activities of the group. Copyright © 2012 Elsevier Ltd. All rights reserved.

46 CFR Sec. 9 - Communications.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 8 2011-10-01 2011-10-01 false Communications. Sec. 9 Section 9 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION B-CONTROL AND UTILIZATION OF PORTS RESTRICTIONS UPON THE TRANSFER OR CHANGE IN USE OR IN TERMS GOVERNING UTILIZATION OF PORT FACILITIES Sec. 9 Communications. Communications...

46 CFR Sec. 9 - Communications.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 8 2012-10-01 2012-10-01 false Communications. Sec. 9 Section 9 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION B-CONTROL AND UTILIZATION OF PORTS RESTRICTIONS UPON THE TRANSFER OR CHANGE IN USE OR IN TERMS GOVERNING UTILIZATION OF PORT FACILITIES Sec. 9 Communications. Communications...

46 CFR Sec. 9 - Communications.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 8 2013-10-01 2013-10-01 false Communications. Sec. 9 Section 9 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION B-CONTROL AND UTILIZATION OF PORTS RESTRICTIONS UPON THE TRANSFER OR CHANGE IN USE OR IN TERMS GOVERNING UTILIZATION OF PORT FACILITIES Sec. 9 Communications. Communications...

46 CFR Sec. 9 - Communications.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 8 2014-10-01 2014-10-01 false Communications. Sec. 9 Section 9 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION B-CONTROL AND UTILIZATION OF PORTS RESTRICTIONS UPON THE TRANSFER OR CHANGE IN USE OR IN TERMS GOVERNING UTILIZATION OF PORT FACILITIES Sec. 9 Communications. Communications...

46 CFR Sec. 2 - Definitions.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 8 2010-10-01 2010-10-01 false Definitions. Sec. 2 Section 2 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION B-CONTROL AND UTILIZATION OF PORTS FEDERAL PORT CONTROLLERS Sec. 2... department of the United States) in time of emergency to meet the needs of the national defense and maintain...

46 CFR Sec. 12 - Audit.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 8 2010-10-01 2010-10-01 false Audit. Sec. 12 Section 12 Shipping MARITIME... TRANSACTIONS UNDER AGENCY AGREEMENTS Reports and Audit Sec. 12 Audit. (a) The owner will audit as currently as possible subsequent to audit by the agent, all documents relating to the activities, maintenance and...

46 CFR Sec. 5 - Accounting.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 8 2010-10-01 2010-10-01 false Accounting. Sec. 5 Section 5 Shipping MARITIME... Sec. 5 Accounting. The General Agent shall record the amounts of compensation paid from the NSA... Accounting Office, at which time the Maritime Administration will take custody of the records. [16 FR 2885...

75 FR 33306 - Notice of Meeting Location Change and Joint Meeting of FASAB and GASB

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-11

... meeting is to discuss: --Measurement Attributes, --Reporting Model, --Cost Accounting, and --Governance... FEDERAL ACCOUNTING STANDARDS ADVISORY BOARD Notice of Meeting Location Change and Joint Meeting of FASAB and GASB AGENCY: Federal Accounting Standards Advisory Board. ACTION: Notice. Board Action...

Sec34p, a Protein Required for Vesicle Tethering to the Yeast Golgi Apparatus, Is in a Complex with Sec35p

PubMed Central

VanRheenen, Susan M.; Cao, Xiaochun; Sapperstein, Stephanie K.; Chiang, Elbert C.; Lupashin, Vladimir V.; Barlowe, Charles; Waters, M. Gerard

1999-01-01

A screen for mutants of Saccharomyces cerevisiae secretory pathway components previously yielded sec34, a mutant that accumulates numerous vesicles and fails to transport proteins from the ER to the Golgi complex at the restrictive temperature (Wuestehube, L.J., R. Duden, A. Eun, S. Hamamoto, P. Korn, R. Ram, and R. Schekman. 1996. Genetics. 142:393–406). We find that SEC34 encodes a novel protein of 93-kD, peripherally associated with membranes. The temperature-sensitive phenotype of sec34-2 is suppressed by the rab GTPase Ypt1p that functions early in the secretory pathway, or by the dominant form of the ER to Golgi complex target-SNARE (soluble N-ethylmaleimide sensitive fusion protein attachment protein receptor)–associated protein Sly1p, Sly1-20p. Weaker suppression is evident upon overexpression of genes encoding the vesicle tethering factor Uso1p or the vesicle-SNAREs Sec22p, Bet1p, or Ykt6p. This genetic suppression profile is similar to that of sec35-1, a mutant allele of a gene encoding an ER to Golgi vesicle tethering factor and, like Sec35p, Sec34p is required in vitro for vesicle tethering. sec34-2 and sec35-1 display a synthetic lethal interaction, a genetic result explained by the finding that Sec34p and Sec35p can interact by two-hybrid analysis. Fractionation of yeast cytosol indicates that Sec34p and Sec35p exist in an ∼750-kD protein complex. Finally, we describe RUD3, a novel gene identified through a genetic screen for multicopy suppressors of a mutation in USO1, which suppresses the sec34-2 mutation as well. PMID:10562277

Cloning of the Pichia anomala SEC61 gene and its expression in a Saccharomyces cerevisiae sec61 mutant.

PubMed

Ruíz, Teresa; De la Rosa, José M; Domínguez, Angel; Rodríguez, Luis

2003-05-01

In several organisms, including Saccharomyces cerevisiae and other yeast species, the product encoded by the SEC61 gene is considered to be the core element of the translocation apparatus within the endoplasmic reticulum membrane through which translocation of secretory and membrane proteins occurs. In this study, we have cloned and characterized the homolog of the SEC61 gene from the yeast Pichia anomala. The cloned gene includes an ORF, interrupted after the first ten nucleotides by an intron of 131 bp, encoding a 479-amino acid putative polypeptide exhibiting homology to the products encoded by different eukaryotic SEC61 genes, particularly to those from other yeast species. We show that the P. anomala SEC61 gene is correctly processed (intron splicing) when expressed in S. cerevisiae and that it is able to complement the thermosensitive phenotype associated with a mutation in the S. cerevisiae SEC61 gene.

Toward an SEC-FERC memorandum of understanding. [SEC-FERC (Securities and Exchange Commission-Federal Energy Regulatory Commission)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Moeller, J.W.

1994-01-01

The Securities and Exchange Commission (SEC) is under fire. For the past year, Congress has criticized its administration of the Public Utility Holding Company Act of 1935 (PUHCA). The criticism might persuade Congress to transfer the administration of PUHCA from the SEC to the Federal Energy Regulatory Commission (FERC). Section I of this article briefly describes the events of the past year and the implications of transfer of PUHCA from the SEC to the FERC. Section II of this article discusses the background relationship of PUHCA, under which the SEC regulates the securities transactions of public utilities, to the Federalmore » Power Act, under which the FERC regulates the rates of public utilities. Section III discusses the case involving the Ohio Power Company, which resulted in a November 1990 decision of the Supreme Court, that recently has highlighted the conflict in jurisdiction between the SEC and the FERC relative to the regulation of public utilities. Section IV discusses the immediate responses to that conflict--a proposed amendment to a FERC regulation and S. 544--and the reasons that neither response is an ideal solution. Section V proposes an alternative to those immediate responses--the Memorandum of Understanding. An Appendix is provided which proposes a draft Memorandum of Understanding between the SEC and the FERC.« less

77 FR 71828 - Meeting of the Advisory Committee; Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2012-12-04

... recommended for inclusion on future Joint Board examinations in actuarial mathematics and methodology referred... meeting of the Advisory Committee on Actuarial Examinations (portions of which will be open to the public... Committee on Actuarial Examinations will meet at the Internal Revenue Service, 1111 Constitution Avenue NW...

78 FR 12762 - Joint Meeting of the Medical Imaging Drugs Advisory Committee and the Oncologic Drugs Advisory...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-02-25

... DEPARTMENT OF HEALTH AND HUMAN SERVICES Food and Drug Administration [Docket No. FDA-2013-N-0001...; Notice of Meeting. AGENCY: Food and Drug Administration, HHS. ACTION: Notice. This notice announces a forthcoming meeting of a public advisory committee of the Food and Drug Administration (FDA). The meeting will...

Introduction to the Centers for Disease Control and Prevention and Healthcare Infection Control Practices Advisory Committee Guideline for Prevention of Surgical Site Infection: Prosthetic Joint Arthroplasty Section.

PubMed

Segreti, John; Parvizi, Javad; Berbari, Elie; Ricks, Philip; Berríos-Torres, Sandra I

Peri-prosthetic joint infection (PJI) is a severe complication of total joint arthroplasty that appears to be increasing as more of these procedures are performed. Numerous risk factors for incisional (superficial and deep) and organ/space (e.g., PJI) surgical site infections (SSIs) have been identified. A better understanding and reversal of modifiable risk factors may lead to a reduction in the incidence of incisional SSI and PJI. The Centers for Disease Control and Prevention (CDC) and the Healthcare Infection Control Practices Advisory Committee (HICPAC) recently updated the national Guideline for Prevention of Surgical Site Infection. The updated guideline applies evidence-based methodology, presents recommendations for potential strategies to reduce the risk of SSI, and includes an arthroplasty-specific section. This article serves to introduce the guideline development process and to complement the Prosthetic Joint Arthroplasty section with background information on PJI-specific economic burden, epidemiology, pathogenesis and microbiology, and risk factor information.

The Immediate Effect of Neuromuscular Joint Facilitation (NJF) Treatment on Hip Muscle Strength.

PubMed

Wang, Hongdan; Huo, Ming; Huang, Qiuchen; Li, Desheng; Maruyama, Hitoshi

2013-11-01

[Purpose] This study investigated the change in hip muscle strength of younger persons after neuromuscular joint facilitation (NJF) treatment. [Subjects] The subjects were 45 healthy young people, who were divided into two groups: a NJF group and a proprioceptive neuromuscular facilitation (PNF) group. The NJF group consisted of 21 subjects (11 males, 10 females), and the PNF group consisted of 24 subjects (11 males, 13 females). [Methods] Participants in the NJF group received NJF treatment. We measured the maximal flexor strength and the maximal extensor strength during isokinetic movement of the hip joint before and after intervention in both groups. The angular velocities used were 60°/sec and 180°/sec. [Results] The NJF group showed significant increases in the maximal flexor strength and the maximal extensor strength after the intervention at each angular velocity. In the PNF group, the maximal flexor strength of 60°/sec and the maximal extensor strength of 180°/sec were significant increases. [Conclusion] These results suggest that there is an immediate effect of NJF intervention on hip muscle strength.

76 FR 2150 - NASA Advisory Council; Education and Public Outreach Committee; Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2011-01-12

... Outreach Committee of the NASA Advisory Council (NAC). DATES: Tuesday, February 8, 2011, 9 a.m. to 5 p.m... Recommendations Status Joint Recommendation with the NAC Commercial Space Committee Committee Work Plan Action...

46 CFR Sec. 16 - Liquidated damages.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 8 2012-10-01 2012-10-01 false Liquidated damages. Sec. 16 Section 16 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION A-NATIONAL SHIPPING AUTHORITY PROCEDURE FOR ACCOMPLISHMENT OF VESSEL REPAIRS UNDER NATIONAL SHIPPING AUTHORITY MASTER LUMP SUM REPAIR CONTRACT-NSA-LUMPSUMREP Sec. 16...

46 CFR Sec. 16 - Liquidated damages.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 8 2013-10-01 2013-10-01 false Liquidated damages. Sec. 16 Section 16 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION A-NATIONAL SHIPPING AUTHORITY PROCEDURE FOR ACCOMPLISHMENT OF VESSEL REPAIRS UNDER NATIONAL SHIPPING AUTHORITY MASTER LUMP SUM REPAIR CONTRACT-NSA-LUMPSUMREP Sec. 16...

46 CFR Sec. 16 - Liquidated damages.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 8 2011-10-01 2011-10-01 false Liquidated damages. Sec. 16 Section 16 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION A-NATIONAL SHIPPING AUTHORITY PROCEDURE FOR ACCOMPLISHMENT OF VESSEL REPAIRS UNDER NATIONAL SHIPPING AUTHORITY MASTER LUMP SUM REPAIR CONTRACT-NSA-LUMPSUMREP Sec. 16...

46 CFR Sec. 16 - Liquidated damages.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 8 2010-10-01 2010-10-01 false Liquidated damages. Sec. 16 Section 16 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION A-NATIONAL SHIPPING AUTHORITY PROCEDURE FOR ACCOMPLISHMENT OF VESSEL REPAIRS UNDER NATIONAL SHIPPING AUTHORITY MASTER LUMP SUM REPAIR CONTRACT-NSA-LUMPSUMREP Sec. 16...

46 CFR Sec. 16 - Liquidated damages.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 8 2014-10-01 2014-10-01 false Liquidated damages. Sec. 16 Section 16 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION A-NATIONAL SHIPPING AUTHORITY PROCEDURE FOR ACCOMPLISHMENT OF VESSEL REPAIRS UNDER NATIONAL SHIPPING AUTHORITY MASTER LUMP SUM REPAIR CONTRACT-NSA-LUMPSUMREP Sec. 16...

76 FR 56797 - Meeting of the Federal Advisory Committee

Federal Register 2010, 2011, 2012, 2013, 2014

2011-09-14

... on Actuarial Examinations. DATES: The meeting will be held on October 21, 2011, from 8:30 a.m. to 5 p... Advisory Committee on Actuarial Examinations will meet at Deloitte, 2901 N. Central Avenue, Suite 1200... and questions that may be recommended for inclusion on future Joint Board examinations in actuarial...

46 CFR Sec. 3 - Standby agreements.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 8 2014-10-01 2014-10-01 false Standby agreements. Sec. 3 Section 3 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION B-CONTROL AND UTILIZATION OF PORTS FEDERAL PORT CONTROLLERS Sec. 3 Standby agreements. The Director, NSA, may negotiate the standard form of service agreement, specified in...

46 CFR Sec. 3 - Standby agreements.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 8 2013-10-01 2013-10-01 false Standby agreements. Sec. 3 Section 3 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION B-CONTROL AND UTILIZATION OF PORTS FEDERAL PORT CONTROLLERS Sec. 3 Standby agreements. The Director, NSA, may negotiate the standard form of service agreement, specified in...

Comparison of exhalation time methods (6 sec vs. 10 sec) of a hand-held exhaled nitric oxide analyzer.

PubMed

Ito, Yasunori; Adachi, Yuichi; Itazawa, Toshiko; Okabe, Yoshie; Adachi, Yoko S; Katsumuma, Toshio; Miyawaki, Toshio

2010-10-01

Standard exhalation time for measuring fractional exhaled nitric oxide (FeNO) is 10 sec, but this is not easy for younger children. We aimed to investigate the agreement between FeNO values during 10-sec (FeNO-10) and 6-sec (FeNO-6) exhalation and the feasibility of measuring FeNO-6, using a hand-held analyzer, NIOX-MINO®. FeNO values measured during 10- and 6-sec (random order) were compared. Success rates of the two different time modes were also evaluated. In 119 asthmatic children (median age 8 years [range 4-15]) who had been already accustomed to NIOX-MINO®, median FeNO-10 (29 ppb [IQR 15.2-42.0]) and FeNO-6 (27 ppb [IQR 16.0-43.5]) did not differ significantly (P = 0.90), and there was a good correlation between both values (r = 0.984, P < 0.001). Mean difference (FeNO-10-FeNO-6) was -0.151 ppb (95% CI: -0.95 to 0.65, limits of agreement: -8.8 to 8.5). In 46 asthmatic children (median age 7 years [range 4-15]) who had never used any FeNO analyzers, all the children aged 8 years and more (n = 21) succeeded in measuring FeNO on both time modes, whereas for children aged younger than 8 years (n = 25) success rates of the 10- and 6-sec mode were 60.0% and 92.0%, respectively. In conclusion, we showed good agreement between FeNO-10 and FeNO-6, and the 6-sec mode of NIOX-MINO® is more feasible than 10-sec mode for measuring FeNO in younger children. © 2010 Wiley-Liss, Inc.

14 CFR Sec. 1-5 - Records.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 14 Aeronautics and Space 4 2013-01-01 2013-01-01 false Records. Sec. 1-5 Section 1-5 Aeronautics... Provisions Sec. 1-5 Records. (a) The general books of account and all books, records, and memoranda which... Accounts. Registers, or other appropriate records, shall be maintained of the history and nature of each...

The Indian Nations At Risk Task Force and the National Advisory Council on Indian Education Joint Issues Sessions Proceedings. Annual Conference of the National Indian Education Association (22nd, San Diego, California, October 15-16, 1990).

ERIC Educational Resources Information Center

National Advisory Council on Indian Education, Washington, DC.

The Indian Nations At Risk Task Force and the National Advisory Council on Indian Education (NACIE) held joint sessions to hear testimony on important issues in American Indian education. This document presents statements given at 15 topical sessions and 3 additional sessions held for special groups. The 15 topics addressed were: teaching Native…

Activation of Rab GTPase Sec4 by its GEF Sec2 is required for prospore membrane formation during sporulation in yeast Saccharomyces cerevisiae.

PubMed

Suda, Yasuyuki; Tachikawa, Hiroyuki; Inoue, Ichiro; Kurita, Tomokazu; Saito, Chieko; Kurokawa, Kazuo; Nakano, Akihiko; Irie, Kenji

2018-02-01

Sec2 activates Sec4 Rab GTPase as a guanine nucleotide exchange factor for the recruitment of downstream effectors to facilitate tethering and fusion of post-Golgi vesicles at the plasma membrane. During the meiosis and sporulation of budding yeast, post-Golgi vesicles are transported to and fused at the spindle pole body (SPB) to form a de novo membrane, called the prospore membrane. Previous studies have revealed the role of the SPB outer surface called the meiotic outer plaque (MOP) in docking and fusion of post-Golgi vesicles. However, the upstream molecular machinery for post-Golgi vesicular fusion that facilitates prospore membrane formation remains enigmatic. Here, we demonstrate that the GTP exchange factor for Sec4, Sec2, participates in the formation of the prospore membrane. A conditional mutant in which the SEC2 expression is shut off during sporulation showed sporulation defects. Inactivation of Sec2 caused Sec4 targeting defects along the prospore membranes, thereby causing insufficient targeting of downstream effectors and cargo proteins to the prospore membrane. These results suggest that the activation of Sec4 by Sec2 is required for the efficient supply of post-Golgi vesicles to the prospore membrane and thus for prospore membrane formation/extension and subsequent deposition of spore wall materials. © FEMS 2017. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

46 CFR Sec. 4 - Funding of operations.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 8 2014-10-01 2014-10-01 false Funding of operations. Sec. 4 Section 4 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION A-NATIONAL SHIPPING AUTHORITY PROCEDURAL RULES FOR FINANCIAL TRANSACTIONS UNDER AGENCY AGREEMENTS Funding of Operations Sec. 4 Funding of operations. Cash...

46 CFR Sec. 4 - Funding of operations.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 8 2013-10-01 2013-10-01 false Funding of operations. Sec. 4 Section 4 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION A-NATIONAL SHIPPING AUTHORITY PROCEDURAL RULES FOR FINANCIAL TRANSACTIONS UNDER AGENCY AGREEMENTS Funding of Operations Sec. 4 Funding of operations. Cash...

46 CFR Sec. 4 - Funding of operations.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 8 2011-10-01 2011-10-01 false Funding of operations. Sec. 4 Section 4 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION A-NATIONAL SHIPPING AUTHORITY PROCEDURAL RULES FOR FINANCIAL TRANSACTIONS UNDER AGENCY AGREEMENTS Funding of Operations Sec. 4 Funding of operations. Cash...

Demonstration of a Specific Escherichia coli SecY–Signal Peptide Interaction†

PubMed Central

Wang, Ligong; Miller, Alexander; Rusch, Sharyn L.; Kendall, Debra A.

2011-01-01

Protein translocation in Escherichia coli is initiated by the interaction of a preprotein with the membrane translocase composed of a motor protein, SecA ATPase, and a membrane-embedded channel, the SecYEG complex. The extent to which the signal peptide region of the preprotein plays a role in SecYEG interactions is unclear, in part because studies in this area typically employ the entire preprotein. Using a synthetic signal peptide harboring a photoaffinity label in its hydrophobic core, we examined this interaction with SecYEG in a detergent micellar environment. The signal peptide was found to specifically bind SecY in a saturable manner and at levels comparable to those that stimulate SecA ATPase activity. Chemical and proteolytic cleavage of cross-linked SecY and analysis of the signal peptide adducts indicate that the binding was primarily to regions of the protein containing transmembrane domains seven and two. The signal peptide–SecY interaction was affected by the presence of SecA and nucleotides in a manner consistent with the transfer of signal peptide to SecY upon nucleotide hydrolysis at SecA. PMID:15476412

46 CFR Sec. 3 - Accounting for revenues.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 8 2010-10-01 2010-10-01 false Accounting for revenues. Sec. 3 Section 3 Shipping... FINANCIAL TRANSACTIONS UNDER AGENCY AGREEMENTS Accounting for Revenues Sec. 3 Accounting for revenues. (a... shipper, consignee, weight or measurement, freight rate and basis (whether the freight rate applies on...

36 CFR 67.1 - Sec. 48(g) and Sec. 170(h) of the Internal Revenue Code of 1986.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 36 Parks, Forests, and Public Property 1 2010-07-01 2010-07-01 false Sec. 48(g) and Sec. 170(h) of the Internal Revenue Code of 1986. 67.1 Section 67.1 Parks, Forests, and Public Property NATIONAL PARK..., Alaska 99503: Alaska Mid-Atlantic Regional Office, National Park Service, U.S. Customs House, Second...

46 CFR Sec. 2 - Stand-by agreements.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 8 2013-10-01 2013-10-01 false Stand-by agreements. Sec. 2 Section 2 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION B-CONTROL AND UTILIZATION OF PORTS OPERATING CONTRACT Sec. 2 Stand-by agreements. The Director NSA, Maritime Administration, in advance of an emergency, may negotiate...

46 CFR Sec. 2 - Stand-by agreements.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 8 2014-10-01 2014-10-01 false Stand-by agreements. Sec. 2 Section 2 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION B-CONTROL AND UTILIZATION OF PORTS OPERATING CONTRACT Sec. 2 Stand-by agreements. The Director NSA, Maritime Administration, in advance of an emergency, may negotiate...

Effects of hand grip exercise on shoulder joint internal rotation and external rotation peak torque.

PubMed

Lee, Dong-Rour; Jong-Soon Kim, Laurentius

2016-08-10

The goal of this study is to analyze the effects of hand grip training on shoulder joint internal rotation (IR)/external rotation (ER) peak torque for healthy people. The research was conducted on 23 healthy adults in their 20 s-30 s who volunteered to participate in the experiment. Hand grip power test was performed on both hands of the research subjects before/after the test to study changes in hand grip power. Isokinetic machine was used to measure the concentric IRPT (internal rotation peak torque) and concentric ERPT (external rotation peak torque) at the velocity of 60°/sec, 90°/sec, and 180°/sec before/after the test. Hand grip training was performed daily on the subject's right hand only for four weeks according to exercise program. Finally, hand grip power of both hands and the maximum torque values of shoulder joint IR/ER were measured before/after the test and analyzed. There was a statistically significant difference in the hand grip power of the right hand, which was subject to hand grip training, after the experiment. Also, statistically significant difference for shoulder ERPT was found at 60°/sec. Hand grip training has a positive effect on shoulder joint IRPT/ERPT and therefore can help strengthen muscles around the shoulder without using weight on the shoulder. Consequently, hand grip training would help maintain strengthen the muscles around the shoulder in the early phase of rehabilitation process after shoulder surgery.

46 CFR Sec. 4 - Method of payment.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 8 2011-10-01 2011-10-01 false Method of payment. Sec. 4 Section 4 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION A-NATIONAL SHIPPING AUTHORITY PROCEDURE TO BE FOLLOWED BY GENERAL... Sec. 4 Method of payment. The General Agent shall prepare check drawn on the NSA Special bank account...

14 CFR Sec. 1-6 - Accounting entities.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Accounting entities. Sec. 1-6 Section 1-6... REGULATIONS UNIFORM SYSTEM OF ACCOUNTS AND REPORTS FOR LARGE CERTIFICATED AIR CARRIERS General Accounting Provisions Sec. 1-6 Accounting entities. (a) Separate accounting records shall be maintained for each air...

46 CFR Sec. 7 - Job order numbering.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 8 2011-10-01 2011-10-01 false Job order numbering. Sec. 7 Section 7 Shipping MARITIME... REPAIRS UNDER NATIONAL SHIPPING AUTHORITY MASTER LUMP SUM REPAIR CONTRACT-NSA-LUMPSUMREP Sec. 7 Job order numbering. (a) The NSA-LUMPSUMREP Contract number shall be inserted in every job order and supplemental job...

46 CFR Sec. 7 - Job order numbering.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 8 2010-10-01 2010-10-01 false Job order numbering. Sec. 7 Section 7 Shipping MARITIME... REPAIRS UNDER NATIONAL SHIPPING AUTHORITY MASTER LUMP SUM REPAIR CONTRACT-NSA-LUMPSUMREP Sec. 7 Job order numbering. (a) The NSA-LUMPSUMREP Contract number shall be inserted in every job order and supplemental job...

46 CFR Sec. 7 - Job order numbering.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 8 2013-10-01 2013-10-01 false Job order numbering. Sec. 7 Section 7 Shipping MARITIME... REPAIRS UNDER NATIONAL SHIPPING AUTHORITY MASTER LUMP SUM REPAIR CONTRACT-NSA-LUMPSUMREP Sec. 7 Job order numbering. (a) The NSA-LUMPSUMREP Contract number shall be inserted in every job order and supplemental job...

46 CFR Sec. 7 - Job order numbering.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 8 2014-10-01 2014-10-01 false Job order numbering. Sec. 7 Section 7 Shipping MARITIME... REPAIRS UNDER NATIONAL SHIPPING AUTHORITY MASTER LUMP SUM REPAIR CONTRACT-NSA-LUMPSUMREP Sec. 7 Job order numbering. (a) The NSA-LUMPSUMREP Contract number shall be inserted in every job order and supplemental job...

46 CFR Sec. 7 - Job order numbering.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 8 2012-10-01 2012-10-01 false Job order numbering. Sec. 7 Section 7 Shipping MARITIME... REPAIRS UNDER NATIONAL SHIPPING AUTHORITY MASTER LUMP SUM REPAIR CONTRACT-NSA-LUMPSUMREP Sec. 7 Job order numbering. (a) The NSA-LUMPSUMREP Contract number shall be inserted in every job order and supplemental job...

14 CFR Sec. 2-4 - Accounting period.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Accounting period. Sec. 2-4 Section 2-4... REGULATIONS UNIFORM SYSTEM OF ACCOUNTS AND REPORTS FOR LARGE CERTIFICATED AIR CARRIERS General Accounting Provisions Sec. 2-4 Accounting period. (a) The accounting year of each air carrier subject to this Uniform...

Society Influencing Science: The role of the Transdisciplinary Advisory Board (TAB) of the European Joint Programming Initiative on Climate.

NASA Astrophysics Data System (ADS)

Noone, K. J.; Manderscheid, P.; Monfray, P.

2017-12-01

It is becoming increasingly apparent that the separation between science and the rest of society is not helping us find solutions to "wicked" problems like climate change or achieving the Sustainable Development Goals. It is clear that a broader approach to research is necessary - one that includes stakeholders in the research process itself. What is unclear is how best to do this. The Transdisciplinary Advisory Board (TAB) of the European Joint Programming Initiative on Climate (JPI Climate) is an example of scientists and stakeholders working together to frame climate research and move the results of scientific research into decision support. JPI Climate is a consortium of 12 European countries (with partners from nine more countries) and is a major funding channel and forum for climate research in Europe. The TAB has an equal number of stakeholders and researchers from 10 different European countries, has an even gender balance, and its members have widely differing backgrounds. The TAB provides input and advice to the governing board of JPI Climate, and influences both the strategic planning for this funding initiative as well as specific calls for proposals issued through the consortium. In addition to its advisory role, the TAB explores the transdisciplinary process itself, expanding the boundaries of how stakeholders and science can interact positively. The TAB is a two-way mechanism through which stakeholders can help improve research and science can help improve society. We will give examples of the spectrum of how the TAB provides mutual influence between stakeholders and science - from helping to draft 10-year research strategies to helping advance the uptake of climate research into the private and policy sectors.

14 CFR Sec. 1-6 - Accounting entities.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 4 2011-01-01 2011-01-01 false Accounting entities. Sec. 1-6 Section 1-6... Provisions Sec. 1-6 Accounting entities. (a) Separate accounting records shall be maintained for each air transport entity for which separate reports to the BTS are required to be made by sections 21(g) and for...

Dynamic distribution of the SecA and SecY translocase subunits and septal localization of the HtrA surface chaperone/protease during Streptococcus pneumoniae D39 cell division.

PubMed

Tsui, Ho-Ching Tiffany; Keen, Susan K; Sham, Lok-To; Wayne, Kyle J; Winkler, Malcolm E

2011-01-01

The Sec translocase pathway is the major route for protein transport across and into the cytoplasmic membrane of bacteria. Previous studies reported that the SecA translocase ATP-binding subunit and the cell surface HtrA protease/chaperone formed a single microdomain, termed "ExPortal," in some species of ellipsoidal (ovococcus) Gram-positive bacteria, including Streptococcus pyogenes. To investigate the generality of microdomain formation, we determined the distribution of SecA and SecY by immunofluorescent microscopy in Streptococcus pneumoniae (pneumococcus), which is an ovococcus species evolutionarily distant from S. pyogenes. In the majority (≥ 75%) of exponentially growing cells, S. pneumoniae SecA (SecA (Spn)) and SecY (Spn) located dynamically in cells at different stages of division. In early divisional cells, both Sec subunits concentrated at equators, which are future sites of constriction. Further along in division, SecA(Spn) and SecY(Spn) remained localized at mid-cell septa. In late divisional cells, both Sec subunits were hemispherically distributed in the regions between septa and the future equators of dividing cells. In contrast, the HtrA (Spn) homologue localized to the equators and septa of most (> 90%) dividing cells, whereas the SrtA(Spn) sortase located over the surface of cells in no discernable pattern. This dynamic pattern of Sec distribution was not perturbed by the absence of flotillin family proteins, but was largely absent in most cells in early stationary phase and in cls mutants lacking cardiolipin synthase. These results do not support the existence of an ExPortal microdomain in S. pneumoniae. Instead, the localization of the pneumococcal Sec translocase depends on the stage of cell division and anionic phospholipid content. Two patterns of Sec translocase distribution, an ExPortal microdomain in certain ovococcus-shaped species like Streptococcus pyogenes and a spiral pattern in rod-shaped species like Bacillus subtilis, have

Field joint environmental protection system vibration/pressurization qualification

NASA Technical Reports Server (NTRS)

Cook, M.

1989-01-01

The procedures used and results obtained from vibration testing the redesigned solid rocket motor (RSRM) field joint environmental protection system (FJEPS), hereafter referred to as the joint protection system (JPS) are documented. The major purposes were to certify that the flight-designed JPS will withstand the dynamic environmental conditions of the redesigned solid rocket booster, and to certify that the cartridge check valve (vent valve) will relieve pressure build-up under the JPS during the initial 120 sec of flight. Also, an evaluation of the extruded cork insulation bonding was performed after the vibration testing.

CoSEC: Connecting Living With a Star Research

NASA Astrophysics Data System (ADS)

Hurlburt, N.; Freeland, S.; Bose, P.; Zimdars, A.; Slater, G.

2006-12-01

The Collaborative Sun-Earth Connector (CoSEC) provide the means for heliophysics researchers to compose the data sources and processing services published by their peers into processing workflows that reliably generate publication-worthy data. It includes: composition of computational and data services into easy-to- read workflows with data quality and version traceability; straightforward translation of existing services into workflow components, and advertisement of those components to other members of the CoSEC community; annotation of published services with functional attributes to enable discovery of capabilities required by particular workflows and identify peer subgroups in the CoSEC community; and annotation of published services with nonfunctional attributes to enable selection on the basis of quality of service (QoS). We present an overview and demonstration of the CoSEC system, discuss applications, the lessons learned and future developments.

75 FR 11189 - Bureau of Health Professions; All Advisory Committee Meeting; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-10

... DEPARTMENT OF HEALTH AND HUMAN SERVICES Health Resources and Services Administration Bureau of... Linkages (ACICBL), and the National Advisory Council on Nurse Education and Practice (NACNEP)] to continue their joint work on interdisciplinary education and practice, and to also discuss and identify future...

77 FR 30289 - Federal Advisory Committee Act; Technological Advisory Council

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-22

... FEDERAL COMMUNICATIONS COMMISSION Federal Advisory Committee Act; Technological Advisory Council... Communications Commission's (FCC) Technological Advisory Council will hold a meeting on Wednesday, June 27, 2012... INFORMATION: The FCC Technological Advisory Council proposed a new work agenda for the [[Page 30290

The mechanism of protein export enhancement by the SecDF membrane component

PubMed Central

Tsukazaki, Tomoya; Nureki, Osamu

2011-01-01

Protein transport across membranes is a fundamental and essential cellular activity in all organisms. In bacteria, protein export across the cytoplasmic membrane, driven by dynamic interplays between the protein-conducting SecYEG channel (Sec translocon) and the SecA ATPase, is enhanced by the proton motive force (PMF) and a membrane-integrated Sec component, SecDF. However, the structure and function of SecDF have remained unclear. We solved the first crystal structure of SecDF, consisting of a pseudo-symmetrical 12-helix transmembrane domain and two protruding periplasmic domains. Based on the structural features, we proposed that SecDF functions as a membrane-integrated chaperone, which drives protein movement without using the major energetic currency, ATP, but with remarkable cycles of conformational changes, powered by the proton gradient across the membrane. By a series of biochemical and biophysical approaches, several functionally important residues in the transmembrane region have been identified and our model of the SecDF function has been verified. PMID:27857601

Transposon-disruption of a maize nuclear gene, tha1, encoding a chloroplast SecA homologue: in vivo role of cp-SecA in thylakoid protein targeting.

PubMed

Voelker, R; Mendel-Hartvig, J; Barkan, A

1997-02-01

A nuclear mutant of maize, tha1, which exhibited defects in the translocation of proteins across the thylakoid membrane, was described previously. A transposon insertion at the tha1 locus facilitated the cloning of portions of the tha1 gene. Strong sequence similarity with secA genes from bacteria, pea and spinach indicates that tha1 encodes a SecA homologue (cp-SecA). The tha1-ref allele is either null or nearly so, in that tha1 mRNA is undetectable in mutant leaves and cp-SecA accumulation is reduced > or = 40-fold. These results, in conjunction with the mutant phenotype described previously, demonstrate that cp-SecA functions in vivo to facilitate the translocation of OEC33, PSI-F and plastocyanin but does not function in the translocation of OEC23 and OEC16. Our results confirm predictions for cp-SecA function made from the results of in vitro experiments and establish several new functions for cp-SecA, including roles in the targeting of a chloroplast-encoded protein, cytochrome f, and in protein targeting in the etioplast, a nonphotosynthetic plastid type. Our finding that the accumulation of properly targeted plastocyanin and cytochrome f in tha1-ref thylakoid membranes is reduced only a few-fold despite the near or complete absence of cp-SecA suggests that cp-SecA facilitates but is not essential in vivo for their translocation across the membrane.

46 CFR Sec. 15 - Subcontracts.

Code of Federal Regulations, 2012 CFR

2012-10-01

... REPAIRS UNDER NATIONAL SHIPPING AUTHORITY MASTER LUMP SUM REPAIR CONTRACT-NSA-LUMPSUMREP Sec. 15 Subcontracts. Under Article 29 of the NSA-LUMPSUMREP Contract, the Contractor is authorized to subcontract...

46 CFR Sec. 15 - Subcontracts.

Code of Federal Regulations, 2013 CFR

2013-10-01

... REPAIRS UNDER NATIONAL SHIPPING AUTHORITY MASTER LUMP SUM REPAIR CONTRACT-NSA-LUMPSUMREP Sec. 15 Subcontracts. Under Article 29 of the NSA-LUMPSUMREP Contract, the Contractor is authorized to subcontract...

46 CFR Sec. 15 - Subcontracts.

Code of Federal Regulations, 2014 CFR

2014-10-01

... REPAIRS UNDER NATIONAL SHIPPING AUTHORITY MASTER LUMP SUM REPAIR CONTRACT-NSA-LUMPSUMREP Sec. 15 Subcontracts. Under Article 29 of the NSA-LUMPSUMREP Contract, the Contractor is authorized to subcontract...

46 CFR Sec. 15 - Subcontracts.

Code of Federal Regulations, 2011 CFR

2011-10-01

... REPAIRS UNDER NATIONAL SHIPPING AUTHORITY MASTER LUMP SUM REPAIR CONTRACT-NSA-LUMPSUMREP Sec. 15 Subcontracts. Under Article 29 of the NSA-LUMPSUMREP Contract, the Contractor is authorized to subcontract...

46 CFR Sec. 15 - Subcontracts.

Code of Federal Regulations, 2010 CFR

2010-10-01

... REPAIRS UNDER NATIONAL SHIPPING AUTHORITY MASTER LUMP SUM REPAIR CONTRACT-NSA-LUMPSUMREP Sec. 15 Subcontracts. Under Article 29 of the NSA-LUMPSUMREP Contract, the Contractor is authorized to subcontract...

46 CFR Sec. 13 - Insurance.

Code of Federal Regulations, 2012 CFR

2012-10-01

... REPAIRS UNDER NATIONAL SHIPPING AUTHORITY MASTER LUMP SUM REPAIR CONTRACT-NSA-LUMPSUMREP Sec. 13 Insurance. Article 9 of the NSA-LUMPSUMREP Contract sets forth the Contractor's liabilities and obligations with...

46 CFR Sec. 13 - Insurance.

Code of Federal Regulations, 2014 CFR

2014-10-01

... REPAIRS UNDER NATIONAL SHIPPING AUTHORITY MASTER LUMP SUM REPAIR CONTRACT-NSA-LUMPSUMREP Sec. 13 Insurance. Article 9 of the NSA-LUMPSUMREP Contract sets forth the Contractor's liabilities and obligations with...

46 CFR Sec. 13 - Insurance.

Code of Federal Regulations, 2011 CFR

2011-10-01

... REPAIRS UNDER NATIONAL SHIPPING AUTHORITY MASTER LUMP SUM REPAIR CONTRACT-NSA-LUMPSUMREP Sec. 13 Insurance. Article 9 of the NSA-LUMPSUMREP Contract sets forth the Contractor's liabilities and obligations with...

46 CFR Sec. 13 - Insurance.

Code of Federal Regulations, 2013 CFR

2013-10-01

... REPAIRS UNDER NATIONAL SHIPPING AUTHORITY MASTER LUMP SUM REPAIR CONTRACT-NSA-LUMPSUMREP Sec. 13 Insurance. Article 9 of the NSA-LUMPSUMREP Contract sets forth the Contractor's liabilities and obligations with...

46 CFR Sec. 13 - Insurance.

Code of Federal Regulations, 2010 CFR

2010-10-01

... REPAIRS UNDER NATIONAL SHIPPING AUTHORITY MASTER LUMP SUM REPAIR CONTRACT-NSA-LUMPSUMREP Sec. 13 Insurance. Article 9 of the NSA-LUMPSUMREP Contract sets forth the Contractor's liabilities and obligations with...

Selective Photoaffinity Labeling Identifies the Signal Peptide Binding Domain on SecA

PubMed Central

Musial-Siwek, Monika; Rusch, Sharyn L.; Kendall, Debra A.

2007-01-01

SecA, an ATPase crucial to the Sec-dependent translocation machinery in Escherichia coli, recognizes and directly binds the N-terminal signal peptide of an exported preprotein. This interaction plays a central role in the targeting and transport of preproteins via the SecYEG channel. Here we identify the Signal Peptide Binding Groove (SPBG) on SecA addressing a key issue regarding the SecA-preprotein interaction. We employ a synthetic signal peptide containing the photoreactive benzoylphenylalanine to efficiently and specifically label SecA containing a unique Factor Xa site. Comparison of the photolabeled fragment from the subsequent proteolysis of several SecAs, which vary only in the location of the Factor Xa site, reveals one 53-residue segment in common with the entire series. The covalently modified SecA segment produced is the same in aqueous solution and in lipid vesicles. This spans amino acids 269 to 322 of the E. coli protein, which is distinct from a previously proposed signal peptide binding site, and contributes to a hydrophobic peptide binding groove evident in molecular models of SecA. PMID:17084862

75 FR 22121 - Request for Public Comment on the Scope of Viewpoints Represented on the Industry Trade Advisory...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-27

... of the trade advisory committee system. As part of this joint review, USTR and Commerce are seeking....commerce.gov ). Dated: April 20, 2010. Nicole Y. Lamb-Hale, Assistant Secretary for Manufacturing and...

10 CFR Appendix A to Subpart A of... - Selected Provisions of the Atomic Energy Act of 1954, as Amended, Sec. 141 (42 U.S.C. 2161), Sec...

Code of Federal Regulations, 2013 CFR

2013-01-01

... 10 Energy 4 2013-01-01 2013-01-01 false Selected Provisions of the Atomic Energy Act of 1954, as Amended, Sec. 141 (42 U.S.C. 2161), Sec. 145 (42 U.S.C. 2165), Sec. 161 (42 U.S.C. 2201) A Appendix A to... to Subpart A of Part 710—Selected Provisions of the Atomic Energy Act of 1954, as Amended, Sec. 141...

10 CFR Appendix A to Subpart A of... - Selected Provisions of the Atomic Energy Act of 1954, as Amended, Sec. 141 (42 U.S.C. 2161), Sec...

Code of Federal Regulations, 2014 CFR

2014-01-01

... 10 Energy 4 2014-01-01 2014-01-01 false Selected Provisions of the Atomic Energy Act of 1954, as Amended, Sec. 141 (42 U.S.C. 2161), Sec. 145 (42 U.S.C. 2165), Sec. 161 (42 U.S.C. 2201) A Appendix A to... to Subpart A of Part 710—Selected Provisions of the Atomic Energy Act of 1954, as Amended, Sec. 141...

10 CFR Appendix A to Subpart A of... - Selected Provisions of the Atomic Energy Act of 1954, as Amended, Sec. 141 (42 U.S.C. 2161), Sec...

Code of Federal Regulations, 2012 CFR

2012-01-01

... 10 Energy 4 2012-01-01 2012-01-01 false Selected Provisions of the Atomic Energy Act of 1954, as Amended, Sec. 141 (42 U.S.C. 2161), Sec. 145 (42 U.S.C. 2165), Sec. 161 (42 U.S.C. 2201) A Appendix A to... to Subpart A of Part 710—Selected Provisions of the Atomic Energy Act of 1954, as Amended, Sec. 141...

10 CFR Appendix A to Subpart A of... - Selected Provisions of the Atomic Energy Act of 1954, as Amended, Sec. 141 (42 U.S.C. 2161), Sec...

Code of Federal Regulations, 2011 CFR

2011-01-01

... 10 Energy 4 2011-01-01 2011-01-01 false Selected Provisions of the Atomic Energy Act of 1954, as Amended, Sec. 141 (42 U.S.C. 2161), Sec. 145 (42 U.S.C. 2165), Sec. 161 (42 U.S.C. 2201) A Appendix A to... to Subpart A of Part 710—Selected Provisions of the Atomic Energy Act of 1954, as Amended, Sec. 141...

10 CFR Appendix A to Subpart A of... - Selected Provisions of the Atomic Energy Act of 1954, as Amended, Sec. 141 (42 U.S.C. 2161), Sec...

Code of Federal Regulations, 2010 CFR

2010-01-01

... 10 Energy 4 2010-01-01 2010-01-01 false Selected Provisions of the Atomic Energy Act of 1954, as Amended, Sec. 141 (42 U.S.C. 2161), Sec. 145 (42 U.S.C. 2165), Sec. 161 (42 U.S.C. 2201) A Appendix A to... to Subpart A of Part 710—Selected Provisions of the Atomic Energy Act of 1954, as Amended, Sec. 141...

Secretome Analysis Defines the Major Role of SecDF in Staphylococcus aureus Virulence

PubMed Central

Quiblier, Chantal; Seidl, Kati; Roschitzki, Bernd; Zinkernagel, Annelies S.; Berger-Bächi, Brigitte; Senn, Maria M.

2013-01-01

The Sec pathway plays a prominent role in protein export and membrane insertion, including the secretion of major bacterial virulence determinants. The accessory Sec constituent SecDF has been proposed to contribute to protein export. Deletion of Staphylococcus aureus secDF has previously been shown to reduce resistance, to alter cell separation, and to change the expression of certain virulence factors. To analyse the impact of the secDF deletion in S. aureus on protein secretion, a quantitative secretome analysis was performed. Numerous Sec signal containing proteins involved in virulence were found to be decreased in the supernatant of the secDF mutant. However, two Sec-dependent hydrolases were increased in comparison to the wild type, suggesting additional indirect, regulatory effects to occur upon deletion of secDF. Adhesion, invasion, and cytotoxicity of the secDF mutant were reduced in human umbilical vein endothelial cells. Virulence was significantly reduced using a Galleria mellonella insect model. Altogether, SecDF is a promising therapeutic target for controlling S. aureus infections. PMID:23658837

SEC sensor parametric test and evaluation system

NASA Technical Reports Server (NTRS)

1978-01-01

This system provides the necessary automated hardware required to carry out, in conjunction with the existing 70 mm SEC television camera, the sensor evaluation tests which are described in detail. The Parametric Test Set (PTS) was completed and is used in a semiautomatic data acquisition and control mode to test the development of the 70 mm SEC sensor, WX 32193. Data analysis of raw data is performed on the Princeton IBM 360-91 computer.

76 FR 22395 - Federal Advisory Committee Act; Open Internet Advisory Committee

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-21

... FEDERAL COMMUNICATIONS COMMISSION Federal Advisory Committee Act; Open Internet Advisory Committee... Advisory Committee, known as the ``Open Internet Advisory Committee'' (hereinafter ``the Committee''), is... effects of the FCC's Open Internet rules (available at http://www.fcc.gov/Daily_Releases/Daily_Business...

The European Society of Regional Anaesthesia and Pain Therapy and the American Society of Regional Anesthesia and Pain Medicine Joint Committee Practice Advisory on Controversial Topics in Pediatric Regional Anesthesia.

PubMed

Ivani, Giorgio; Suresh, Santhanam; Ecoffey, Claude; Bosenberg, Adrian; Lonnqvist, Per-Anne; Krane, Elliot; Veyckemans, Francis; Polaner, David M; Van de Velde, Marc; Neal, Joseph M

2015-01-01

Some topics in the clinical management of regional anesthesia in children remain controversial. To evaluate and come to a consensus regarding some of these topics, The European Society of Regional Anaesthesia and Pain Therapy (ESRA) and the American Society of Regional Anesthesia and Pain Medicine (ASRA) developed a joint committee practice advisory on pediatric regional anesthesia (PRA). Representatives from both ASRA and ESRA comprised the joint committee practice advisory on PRA. Evidence-based recommendations were based on a systematic search of the literature. In cases where no literature was available, expert opinion was elicited. Experts selected controversial topics in PRA. The performance of PRA under general anesthesia or deep sedation is associated with acceptable safety and should be viewed as the standard of care (Evidence B2 and Evidence B3). Because of the difficulty interpreting a negative test dose, the use of test dosing should remain discretionary (Evidence B4). The use of either air-loss of resistance or saline-loss of resistance techniques is supported by expert opinion, but the literature supporting one technique over the other is sparse and controversial; when used appropriately, each technique may be safely used in children. There are no current evidence-based data that the use of RA increases the risk for acute compartment syndrome or delays its diagnosis in children. High-level evidence is not yet available for the topics evaluated, and most recommendations are based on Evidence B studies. The ESRA/ASRA recommendations intend to provide guidance for the safe practice of regional anesthesia in children.

46 CFR Sec. 8 - Extra work and changes.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 8 2010-10-01 2010-10-01 false Extra work and changes. Sec. 8 Section 8 Shipping... Sec. 8 Extra work and changes. (a) At any time after the award of an original job order and during the time the work thereunder is being performed, additional or extra work or changes in the work covered by...

46 CFR Sec. 8 - Extra work and changes.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 8 2011-10-01 2011-10-01 false Extra work and changes. Sec. 8 Section 8 Shipping... Sec. 8 Extra work and changes. (a) At any time after the award of an original job order and during the time the work thereunder is being performed, additional or extra work or changes in the work covered by...

77 FR 30595 - Further Definition of “Swap Dealer,” “Security-Based Swap Dealer,” “Major Swap Participant...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-23

...In accordance with the Dodd-Frank Wall Street Reform and Consumer Protection Act of 2010 (``Dodd-Frank Act''), the Commodity Futures Trading Commission (``CFTC'') and the Securities and Exchange Commission (``SEC'') (collectively, the ``Commissions''), in consultation with the Board of Governors of the Federal Reserve System (``Board''), are adopting new rules and interpretive guidance under the Commodity Exchange Act (``CEA''), and the Securities Exchange Act of 1934 (``Exchange Act''), to further define the terms ``swap dealer,'' ``security-based swap dealer,'' ``major swap participant,'' ``major security-based swap participant,'' and ``eligible contract participant.''

75 FR 8310 - Federal Advisory Committee; Transformation Advisory Group; Closed Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2010-02-24

... DEPARTMENT OF DEFENSE Office of the Secretary Federal Advisory Committee; Transformation Advisory... announces that the Transformation Advisory Group will hold a closed meeting on March 11, 2010. DATES: The... written statements to the membership of the Transformation Advisory Group at any time or in response to...

78 FR 51191 - Government-wide Travel Advisory Committee (GTAC); Public Advisory Committee Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2013-08-20

...] Government-wide Travel Advisory Committee (GTAC); Public Advisory Committee Meeting AGENCY: Office of... Travel Advisory Committee (GTAC) (the Committee), is a Federal Advisory Committee established in...: Ms. Marcerto Barr, Designated Federal Officer (DFO), Government-wide Travel Advisory Committee (GTAC...

46 CFR Sec. 2 - Terms.

Code of Federal Regulations, 2013 CFR

2013-10-01

... PREPARATION OF INVOICES AND PAYMENT OF COMPENSATION PURSUANT TO PROVISIONS OF NSA ORDER NO. 47 Sec. 2 Terms. The terms employed in this order shall have the same meaning as those contained in NSA Order No. 47. ...

46 CFR Sec. 2 - Terms.

Code of Federal Regulations, 2011 CFR

2011-10-01

... PREPARATION OF INVOICES AND PAYMENT OF COMPENSATION PURSUANT TO PROVISIONS OF NSA ORDER NO. 47 Sec. 2 Terms. The terms employed in this order shall have the same meaning as those contained in NSA Order No. 47. ...

46 CFR Sec. 2 - Terms.

Code of Federal Regulations, 2012 CFR

2012-10-01

... PREPARATION OF INVOICES AND PAYMENT OF COMPENSATION PURSUANT TO PROVISIONS OF NSA ORDER NO. 47 Sec. 2 Terms. The terms employed in this order shall have the same meaning as those contained in NSA Order No. 47. ...

46 CFR Sec. 2 - Terms.

Code of Federal Regulations, 2014 CFR

2014-10-01

... PREPARATION OF INVOICES AND PAYMENT OF COMPENSATION PURSUANT TO PROVISIONS OF NSA ORDER NO. 47 Sec. 2 Terms. The terms employed in this order shall have the same meaning as those contained in NSA Order No. 47. ...

46 CFR Sec. 2 - Terms.

Code of Federal Regulations, 2010 CFR

2010-10-01

... PREPARATION OF INVOICES AND PAYMENT OF COMPENSATION PURSUANT TO PROVISIONS OF NSA ORDER NO. 47 Sec. 2 Terms. The terms employed in this order shall have the same meaning as those contained in NSA Order No. 47. ...

14 CFR Sec. 19-7 - Passenger origin-destination survey.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 14 Aeronautics and Space 4 2013-01-01 2013-01-01 false Passenger origin-destination survey. Sec... AIR CARRIERS Operating Statistics Classifications Sec. 19-7 Passenger origin-destination survey. (a... carriers) shall participate in a Passenger Origin-Destination (O & D) Survey covering domestic and...

14 CFR Sec. 19-7 - Passenger origin-destination survey.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 14 Aeronautics and Space 4 2014-01-01 2014-01-01 false Passenger origin-destination survey. Sec... AIR CARRIERS Operating Statistics Classifications Sec. 19-7 Passenger origin-destination survey. (a... carriers) shall participate in a Passenger Origin-Destination (O & D) Survey covering domestic and...

Nascent chain-monitored remodeling of the Sec machinery for salinity adaptation of marine bacteria

PubMed Central

Ishii, Eiji; Chiba, Shinobu; Hashimoto, Narimasa; Kojima, Seiji; Homma, Michio; Ito, Koreaki; Akiyama, Yoshinori; Mori, Hiroyuki

2015-01-01

SecDF interacts with the SecYEG translocon in bacteria and enhances protein export in a proton-motive-force-dependent manner. Vibrio alginolyticus, a marine-estuarine bacterium, contains two SecDF paralogs, V.SecDF1 and V.SecDF2. Here, we show that the export-enhancing function of V.SecDF1 requires Na+ instead of H+, whereas V.SecDF2 is Na+-independent, presumably requiring H+. In accord with the cation-preference difference, V.SecDF2 was only expressed under limited Na+ concentrations whereas V.SecDF1 was constitutive. However, it is not the decreased concentration of Na+ per se that the bacterium senses to up-regulate the V.SecDF2 expression, because marked up-regulation of the V.SecDF2 synthesis was observed irrespective of Na+ concentrations under certain genetic/physiological conditions: (i) when the secDF1VA gene was deleted and (ii) whenever the Sec export machinery was inhibited. VemP (Vibrio export monitoring polypeptide), a secretory polypeptide encoded by the upstream ORF of secDF2VA, plays the primary role in this regulation by undergoing regulated translational elongation arrest, which leads to unfolding of the Shine–Dalgarno sequence for translation of secDF2VA. Genetic analysis of V. alginolyticus established that the VemP-mediated regulation of SecDF2 is essential for the survival of this marine bacterium in low-salinity environments. These results reveal that a class of marine bacteria exploits nascent-chain ribosome interactions to optimize their protein export pathways to propagate efficiently under different ionic environments that they face in their life cycles. PMID:26392525

Phosphatidic Acid Sequesters Sec18p from cis-SNARE Complexes to Inhibit Priming.

PubMed

Starr, Matthew L; Hurst, Logan R; Fratti, Rutilio A

2016-10-01

Yeast vacuole fusion requires the activation of cis-SNARE complexes through priming carried out by Sec18p/N-ethylmaleimide sensitive factor and Sec17p/α-SNAP. The association of Sec18p with vacuolar cis-SNAREs is regulated in part by phosphatidic acid (PA) phosphatase production of diacylglycerol (DAG). Inhibition of PA phosphatase activity blocks the transfer of membrane-associated Sec18p to SNAREs. Thus, we hypothesized that Sec18p associates with PA-rich membrane microdomains before transferring to cis-SNARE complexes upon PA phosphatase activity. Here, we examined the direct binding of Sec18p to liposomes containing PA or DAG. We found that Sec18p preferentially bound to liposomes containing PA compared with those containing DAG by approximately fivefold. Additionally, using a specific PA-binding domain blocked Sec18p binding to PA-liposomes and displaced endogenous Sec18p from isolated vacuoles. Moreover, the direct addition of excess PA blocked the priming activity of isolated vacuoles in a manner similar to chemically inhibiting PA phosphatase activity. These data suggest that the conversion of PA to DAG facilitates the recruitment of Sec18p to cis-SNAREs. Purified vacuoles from yeast lacking the PA phosphatase Pah1p showed reduced Sec18p association with cis-SNAREs and complementation with plasmid-encoded PAH1 or recombinant Pah1p restored the interaction. Taken together, this demonstrates that regulating PA concentrations by Pah1p activity controls SNARE priming by Sec18p. © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

46 CFR Sec. 5 - Measures to protect ship's payrolls.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 8 2012-10-01 2012-10-01 false Measures to protect ship's payrolls. Sec. 5 Section 5 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION A-NATIONAL SHIPPING AUTHORITY BONDING OF SHIP'S PERSONNEL Sec. 5 Measures to protect ship's payrolls. (a) General Agents are not required to...

76 FR 71033 - Federal Advisory Committee Act; Technological Advisory Council

Federal Register 2010, 2011, 2012, 2013, 2014

2011-11-16

... FEDERAL COMMUNICATIONS COMMISSION Federal Advisory Committee Act; Technological Advisory Council... Communications Commission's (FCC) Technological Advisory Council will hold a meeting on Tuesday, December 20... in progress and discuss potential agendas for the coming year. The FCC will attempt to accommodate as...

78 FR 69991 - Advisory Committee; Veterinary Medicine Advisory Committee; Termination

Federal Register 2010, 2011, 2012, 2013, 2014

2013-11-22

... DEPARTMENT OF HEALTH AND HUMAN SERVICES Food and Drug Administration 21 CFR Part 14 [Docket No. FDA-2013-N-1380] Advisory Committee; Veterinary Medicine Advisory Committee; Termination AGENCY: Food... announcing the termination of the Veterinary Medicine Advisory Committee. This document removes the...

17 CFR 200.80b - Appendix B-SEC releases.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 17 Commodity and Securities Exchanges 3 2014-04-01 2014-04-01 false Appendix B-SEC releases. 200.80b Section 200.80b Commodity and Securities Exchanges SECURITIES AND EXCHANGE COMMISSION ORGANIZATION... corporate reorganization releases, and the litigation releases are contained in the SEC Docket, which may be...

17 CFR 200.80b - Appendix B-SEC releases.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 17 Commodity and Securities Exchanges 2 2013-04-01 2013-04-01 false Appendix B-SEC releases. 200.80b Section 200.80b Commodity and Securities Exchanges SECURITIES AND EXCHANGE COMMISSION ORGANIZATION... corporate reorganization releases, and the litigation releases are contained in the SEC Docket, which may be...

17 CFR 200.80b - Appendix B-SEC releases.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 17 Commodity and Securities Exchanges 2 2010-04-01 2010-04-01 false Appendix B-SEC releases. 200.80b Section 200.80b Commodity and Securities Exchanges SECURITIES AND EXCHANGE COMMISSION ORGANIZATION... corporate reorganization releases, and the litigation releases are contained in the SEC Docket, which may be...

17 CFR 200.80b - Appendix B-SEC releases.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 17 Commodity and Securities Exchanges 2 2011-04-01 2011-04-01 false Appendix B-SEC releases. 200.80b Section 200.80b Commodity and Securities Exchanges SECURITIES AND EXCHANGE COMMISSION ORGANIZATION... corporate reorganization releases, and the litigation releases are contained in the SEC Docket, which may be...

17 CFR 200.80b - Appendix B-SEC releases.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 17 Commodity and Securities Exchanges 2 2012-04-01 2012-04-01 false Appendix B-SEC releases. 200.80b Section 200.80b Commodity and Securities Exchanges SECURITIES AND EXCHANGE COMMISSION ORGANIZATION... corporate reorganization releases, and the litigation releases are contained in the SEC Docket, which may be...

14 CFR Sec. 2-5 - Revenue and accounting practices.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Revenue and accounting practices. Sec. 2-5... General Accounting Provisions Sec. 2-5 Revenue and accounting practices. (a) Revenue accounting practices... physically verify the reliability of its passenger revenue accounting practice at least once each accounting...

75 FR 43156 - Federal Advisory Committee; Missile Defense Advisory Committee

Federal Register 2010, 2011, 2012, 2013, 2014

2010-07-23

... DEPARTMENT OF DEFENSE Office of the Secretary Federal Advisory Committee; Missile Defense Advisory Committee AGENCY: Missile Defense Agency (MDA), DoD. ACTION: Notice of closed meeting. SUMMARY: Under the... Defense announces that the Missile Defense Advisory Committee will meet on August 4 and 5, 2010, in...

46 CFR Sec. 2 - Description of NSA-WORKSMALREP Contract.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 8 2013-10-01 2013-10-01 false Description of NSA-WORKSMALREP Contract. Sec. 2 Section... REPAIRS-NSA-WORKSMALREP Sec. 2 Description of NSA-WORKSMALREP Contract. This is an individual fixed price contract which may be awarded to any firm not holding an NSA-LUMPSUMREP Contract, as a result of formal...

46 CFR Sec. 2 - Description of NSA-WORKSMALREP Contract.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 8 2012-10-01 2012-10-01 false Description of NSA-WORKSMALREP Contract. Sec. 2 Section... REPAIRS-NSA-WORKSMALREP Sec. 2 Description of NSA-WORKSMALREP Contract. This is an individual fixed price contract which may be awarded to any firm not holding an NSA-LUMPSUMREP Contract, as a result of formal...

46 CFR Sec. 2 - Description of NSA-WORKSMALREP Contract.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 8 2014-10-01 2014-10-01 false Description of NSA-WORKSMALREP Contract. Sec. 2 Section... REPAIRS-NSA-WORKSMALREP Sec. 2 Description of NSA-WORKSMALREP Contract. This is an individual fixed price contract which may be awarded to any firm not holding an NSA-LUMPSUMREP Contract, as a result of formal...

46 CFR Sec. 2 - Description of NSA-WORKSMALREP Contract.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 8 2011-10-01 2011-10-01 false Description of NSA-WORKSMALREP Contract. Sec. 2 Section... REPAIRS-NSA-WORKSMALREP Sec. 2 Description of NSA-WORKSMALREP Contract. This is an individual fixed price contract which may be awarded to any firm not holding an NSA-LUMPSUMREP Contract, as a result of formal...

Financial Journalism under Fire: The SEC and Newsroom Ethics.

ERIC Educational Resources Information Center

Spellman, Robert L.

Although noting that the Securities and Exchange Commission (SEC) has been a valuable ally of journalists, this paper suggests that recent efforts of the SEC in prosecuting the case of R. Foster Winans, Jr., a former writer for the "Wall Street Journal," may be unconstitutional. Following an introduction to the First Amendment issues…

77 FR 41378 - Privacy Act of 1974 System of Records Notice

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-13

... Revenue Service, the Department of Agriculture, the Office of Personnel Management, and to other Federal... social security number and date of birth. CFTC provides elements of that information to Federal..., date of birth, state of birth, country of birth, non CFTC phone number, non CFTC email address, CFTC...

46 CFR Sec. 5 - Measures to protect ship's payrolls.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 8 2010-10-01 2010-10-01 false Measures to protect ship's payrolls. Sec. 5 Section 5... SHIP'S PERSONNEL Sec. 5 Measures to protect ship's payrolls. (a) General Agents are not required to... paying off the crew should be either the Master, or purser, or some other member of the ship's personnel...

46 CFR Sec. 5 - Measures to protect ship's payrolls.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 8 2011-10-01 2011-10-01 false Measures to protect ship's payrolls. Sec. 5 Section 5... SHIP'S PERSONNEL Sec. 5 Measures to protect ship's payrolls. (a) General Agents are not required to... paying off the crew should be either the Master, or purser, or some other member of the ship's personnel...

46 CFR Sec. 5 - Measures to protect ship's payrolls.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 8 2014-10-01 2014-10-01 false Measures to protect ship's payrolls. Sec. 5 Section 5... SHIP'S PERSONNEL Sec. 5 Measures to protect ship's payrolls. (a) General Agents are not required to... paying off the crew should be either the Master, or purser, or some other member of the ship's personnel...

46 CFR Sec. 5 - Measures to protect ship's payrolls.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 8 2013-10-01 2013-10-01 false Measures to protect ship's payrolls. Sec. 5 Section 5... SHIP'S PERSONNEL Sec. 5 Measures to protect ship's payrolls. (a) General Agents are not required to... paying off the crew should be either the Master, or purser, or some other member of the ship's personnel...

46 CFR Sec. 11 - Guarantee obligations.

Code of Federal Regulations, 2013 CFR

2013-10-01

... ACCOMPLISHMENT OF VESSEL REPAIRS UNDER NATIONAL SHIPPING AUTHORITY MASTER LUMP SUM REPAIR CONTRACT-NSA-LUMPSUMREP Sec. 11 Guarantee obligations. (a) Under the provisions of Article 10 of the NSA-LUMPSUMREP Contract...

46 CFR Sec. 11 - Guarantee obligations.

Code of Federal Regulations, 2012 CFR

2012-10-01

... ACCOMPLISHMENT OF VESSEL REPAIRS UNDER NATIONAL SHIPPING AUTHORITY MASTER LUMP SUM REPAIR CONTRACT-NSA-LUMPSUMREP Sec. 11 Guarantee obligations. (a) Under the provisions of Article 10 of the NSA-LUMPSUMREP Contract...

46 CFR Sec. 11 - Guarantee obligations.

Code of Federal Regulations, 2014 CFR

2014-10-01

... ACCOMPLISHMENT OF VESSEL REPAIRS UNDER NATIONAL SHIPPING AUTHORITY MASTER LUMP SUM REPAIR CONTRACT-NSA-LUMPSUMREP Sec. 11 Guarantee obligations. (a) Under the provisions of Article 10 of the NSA-LUMPSUMREP Contract...

46 CFR Sec. 11 - Guarantee obligations.

Code of Federal Regulations, 2011 CFR

2011-10-01

... ACCOMPLISHMENT OF VESSEL REPAIRS UNDER NATIONAL SHIPPING AUTHORITY MASTER LUMP SUM REPAIR CONTRACT-NSA-LUMPSUMREP Sec. 11 Guarantee obligations. (a) Under the provisions of Article 10 of the NSA-LUMPSUMREP Contract...

76 FR 1212 - Joint Biomedical Laboratory Research and Development and Clinical Science Research and...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-01-07

... DEPARTMENT OF VETERANS AFFAIRS Joint Biomedical Laboratory Research and Development and Clinical Science Research and Development Services Scientific Merit Review Board Panel for Eligibility; Notice of Meeting The Department of Veterans Affairs (VA) gives notice under the Public Law 92-463 (Federal Advisory...

75 FR 57833 - Joint Biomedical Laboratory Research and Development and Clinical Science Research and...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-22

... DEPARTMENT OF VETERANS AFFAIRS Joint Biomedical Laboratory Research and Development and Clinical Science Research and Development Services Scientific Merit Review Board; Notice of Meetings The Department of Veterans Affairs gives notice under the Public Law 92-463 (Federal Advisory Committee Act) that...

76 FR 66367 - Joint Biomedical Laboratory Research and Development and Clinical Science Research and...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-26

... DEPARTMENT OF VETERANS AFFAIRS Joint Biomedical Laboratory Research and Development and Clinical Science Research and Development Services Scientific Merit Review Board; Notice of Meetings The Department of Veterans Affairs gives notice under the Public Law 92-463 (Federal Advisory Committee Act) that...

77 FR 26069 - Joint Biomedical Laboratory Research and Development and Clinical Science Research and...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-02

... DEPARTMENT OF VETERANS AFFAIRS Joint Biomedical Laboratory Research and Development and Clinical Science Research and Development Services Scientific Merit Review Board, Notice of Meeting Amendment The Department of Veterans Affairs (VA) gives notice under the Public Law 92-463 (Federal Advisory Committee Act...

76 FR 79273 - Joint Biomedical Laboratory Research and Development and Clinical Science Research and...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-12-21

... DEPARTMENT OF VETERANS AFFAIRS Joint Biomedical Laboratory Research and Development and Clinical Science Research and Development Services Scientific Merit Review Board Panel for Eligibility, Notice of Meeting The Department of Veterans Affairs (VA) gives notice under Public Law 92-463 (Federal Advisory...

46 CFR Sec. 10 - Bonds.

Code of Federal Regulations, 2010 CFR

2010-10-01

... REPAIRS UNDER NATIONAL SHIPPING AUTHORITY MASTER LUMP SUM REPAIR CONTRACT-NSA-LUMPSUMREP Sec. 10 Bonds. (a... awarded work and the furnishing of the performance and payment bonds required by Article 14 of the NSA... of the NSA-LUMPSUMREP Contract, the standard form of individual performance bond (Standard Form 25...

46 CFR Sec. 10 - Bonds.

Code of Federal Regulations, 2011 CFR

2011-10-01

... REPAIRS UNDER NATIONAL SHIPPING AUTHORITY MASTER LUMP SUM REPAIR CONTRACT-NSA-LUMPSUMREP Sec. 10 Bonds. (a... awarded work and the furnishing of the performance and payment bonds required by Article 14 of the NSA... of the NSA-LUMPSUMREP Contract, the standard form of individual performance bond (Standard Form 25...

46 CFR Sec. 10 - Bonds.

Code of Federal Regulations, 2012 CFR

2012-10-01

... REPAIRS UNDER NATIONAL SHIPPING AUTHORITY MASTER LUMP SUM REPAIR CONTRACT-NSA-LUMPSUMREP Sec. 10 Bonds. (a... awarded work and the furnishing of the performance and payment bonds required by Article 14 of the NSA... of the NSA-LUMPSUMREP Contract, the standard form of individual performance bond (Standard Form 25...

46 CFR Sec. 10 - Bonds.

Code of Federal Regulations, 2014 CFR

2014-10-01

... REPAIRS UNDER NATIONAL SHIPPING AUTHORITY MASTER LUMP SUM REPAIR CONTRACT-NSA-LUMPSUMREP Sec. 10 Bonds. (a... awarded work and the furnishing of the performance and payment bonds required by Article 14 of the NSA... of the NSA-LUMPSUMREP Contract, the standard form of individual performance bond (Standard Form 25...

46 CFR Sec. 10 - Bonds.

Code of Federal Regulations, 2013 CFR

2013-10-01

... REPAIRS UNDER NATIONAL SHIPPING AUTHORITY MASTER LUMP SUM REPAIR CONTRACT-NSA-LUMPSUMREP Sec. 10 Bonds. (a... awarded work and the furnishing of the performance and payment bonds required by Article 14 of the NSA... of the NSA-LUMPSUMREP Contract, the standard form of individual performance bond (Standard Form 25...

Protein Export by the Mycobacterial SecA2 System Is Determined by the Preprotein Mature Domain

PubMed Central

Feltcher, Meghan E.; Gibbons, Henry S.; Ligon, Lauren S.

2013-01-01

At the core of the bacterial general secretion (Sec) pathway is the SecA ATPase, which powers translocation of unfolded preproteins containing Sec signal sequences through the SecYEG membrane channel. Mycobacteria have two nonredundant SecA homologs: SecA1 and SecA2. While the essential SecA1 handles “housekeeping” export, the nonessential SecA2 exports a subset of proteins and is required for Mycobacterium tuberculosis virulence. Currently, it is not understood how SecA2 contributes to Sec export in mycobacteria. In this study, we focused on identifying the features of two SecA2 substrates that target them to SecA2 for export, the Ms1704 and Ms1712 lipoproteins of the model organism Mycobacterium smegmatis. We found that the mature domains of Ms1704 and Ms1712, not the N-terminal signal sequences, confer SecA2-dependent export. We also demonstrated that the lipid modification and the extreme N terminus of the mature protein do not impart the requirement for SecA2 in export. We further showed that the Ms1704 mature domain can be efficiently exported by the twin-arginine translocation (Tat) pathway. Because the Tat system exports only folded proteins, this result implies that SecA2 substrates can fold in the cytoplasm and suggests a putative role of SecA2 in enabling export of such proteins. Thus, the mycobacterial SecA2 system may represent another way that bacteria solve the problem of exporting proteins that can fold in the cytoplasm. PMID:23204463

78 FR 28292 - Joint Biomedical Laboratory Research and Development and Clinical Science Research and...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-05-14

... DEPARTMENT OF VETERANS AFFAIRS Joint Biomedical Laboratory Research and Development and Clinical Science Research and Development Services Scientific Merit Review Board; Notice of Meetings; Amendment The Department of Veterans Affairs (VA) gives notice under the Federal Advisory Committee Act, 5 U.S.C. App...

Sec66-Dependent Regulation of Yeast Spindle-Pole Body Duplication Through Pom152

PubMed Central

Katta, Santharam S.; Chen, Jingjing; Gardner, Jennifer M.; Friederichs, Jennifer M.; Smith, Sarah E.; Gogol, Madelaine; Unruh, Jay R.; Slaughter, Brian D.; Jaspersen, Sue L.

2015-01-01

In closed mitotic systems such as Saccharomyces cerevisiae, the nuclear envelope (NE) does not break down during mitosis, so microtubule-organizing centers such as the spindle-pole body (SPB) must be inserted into the NE to facilitate bipolar spindle formation and chromosome segregation. The mechanism of SPB insertion has been linked to NE insertion of nuclear pore complexes (NPCs) through a series of genetic and physical interactions between NPCs and SPB components. To identify new genes involved in SPB duplication and NE insertion, we carried out genome-wide screens for suppressors of deletion alleles of SPB components, including Mps3 and Mps2. In addition to the nucleoporins POM152 and POM34, we found that elimination of SEC66/SEC71/KAR7 suppressed lethality of cells lacking MPS2 or MPS3. Sec66 is a nonessential subunit of the Sec63 complex that functions together with the Sec61 complex in import of proteins into the endoplasmic reticulum (ER). Cells lacking Sec66 have reduced levels of Pom152 protein but not Pom34 or Ndc1, a shared component of the NPC and SPB. The fact that Sec66 but not other subunits of the ER translocon bypass deletion mutants in SPB genes suggests a specific role for Sec66 in the control of Pom152 levels. Based on the observation that sec66∆ does not affect the distribution of Ndc1 on the NE or Ndc1 binding to the SPB, we propose that Sec66-mediated regulation of Pom152 plays an NPC-independent role in the control of SPB duplication. PMID:26510791

Public Files of the SEC.

ERIC Educational Resources Information Center

Woodward, Steve

Some 180 different forms are used by the Securities and Exchange Commission (SEC) to cover the broad range of business activities regulated by the agency. This report examines the forms that are of greatest use to those seeking information about business. These forms are grouped in the following categories: general business information,…

78 FR 42526 - Compliance Policy Guide Sec. 690.800 Salmonella

Federal Register 2010, 2011, 2012, 2013, 2014

2013-07-16

... withdrawal of ``Compliance Policy Guide Sec. 690.700 Salmonella Contamination of Dry Dog Food.'' The... DEPARTMENT OF HEALTH AND HUMAN SERVICES Food and Drug Administration [Docket No. FDA-2010-D-0378] Compliance Policy Guide Sec. 690.800 Salmonella in Food for Animals; Availability AGENCY: Food and Drug...

Accreditation and Academic Freedom. An American Association of University Professors--Council for Higher Education Accreditation Advisory Statement

ERIC Educational Resources Information Center

Council for Higher Education Accreditation, 2013

2013-01-01

This joint American Association of University Professors-Council for Higher Education advisory statement addresses the role that accreditation plays in sustaining and enhancing academic freedom in the context of review of institutions and programs for quality. It offers five suggestions about the role of accreditation with regard to academic…

46 CFR Sec. 12 - Disposition of removed equipment and scrap.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 8 2010-10-01 2010-10-01 false Disposition of removed equipment and scrap. Sec. 12... CONTRACT-NSA-LUMPSUMREP Sec. 12 Disposition of removed equipment and scrap. (a) Article 8 of the NSA-LUMPSUMREP Contract provides that any ship equipment, fuel, lube oil, supplies, stores, furniture, fixtures...

46 CFR Sec. 5 - Disbursements at principal office of agent.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 8 2010-10-01 2010-10-01 false Disbursements at principal office of agent. Sec. 5 Section 5 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION A-NATIONAL SHIPPING AUTHORITY PROCEDURAL RULES FOR FINANCIAL TRANSACTIONS UNDER AGENCY AGREEMENTS Disbursements Sec. 5 Disbursements at principal office of agent. All expenses...

46 CFR Sec. 5 - Accounting.

Code of Federal Regulations, 2013 CFR

2013-10-01

... AGENTS IN PREPARATION OF INVOICES AND PAYMENT OF COMPENSATION PURSUANT TO PROVISIONS OF NSA ORDER NO. 47 Sec. 5 Accounting. The General Agent shall record the amounts of compensation paid from the NSA... compensation paid under sections 3(a), 3(b), 3(c), and 3(d) of NSA Order No. 47. Note: Invoices and account...

46 CFR Sec. 5 - Accounting.

Code of Federal Regulations, 2012 CFR

2012-10-01

... AGENTS IN PREPARATION OF INVOICES AND PAYMENT OF COMPENSATION PURSUANT TO PROVISIONS OF NSA ORDER NO. 47 Sec. 5 Accounting. The General Agent shall record the amounts of compensation paid from the NSA... compensation paid under sections 3(a), 3(b), 3(c), and 3(d) of NSA Order No. 47. Note: Invoices and account...

46 CFR Sec. 5 - Accounting.

Code of Federal Regulations, 2011 CFR

2011-10-01

... AGENTS IN PREPARATION OF INVOICES AND PAYMENT OF COMPENSATION PURSUANT TO PROVISIONS OF NSA ORDER NO. 47 Sec. 5 Accounting. The General Agent shall record the amounts of compensation paid from the NSA... compensation paid under sections 3(a), 3(b), 3(c), and 3(d) of NSA Order No. 47. Note: Invoices and account...

46 CFR Sec. 5 - Accounting.

Code of Federal Regulations, 2014 CFR

2014-10-01

... AGENTS IN PREPARATION OF INVOICES AND PAYMENT OF COMPENSATION PURSUANT TO PROVISIONS OF NSA ORDER NO. 47 Sec. 5 Accounting. The General Agent shall record the amounts of compensation paid from the NSA... compensation paid under sections 3(a), 3(b), 3(c), and 3(d) of NSA Order No. 47. Note: Invoices and account...

76 FR 34667 - Joint Public Roundtable on Proposed Dealer and Major Participant Definitions of Title VII of the...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-06-14

...,'' and collectively, the ``Agencies''). ACTION: Notice of roundtable discussion; request for comment... discussion will be open to the public with seating on a first-come, first-served basis. Members of the public...: 7731946. A transcript of the public roundtable discussion will be published at http://www.cftc.gov/Press...

14 CFR Sec. 2-2 - Basis of allocation between entities.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 4 2011-01-01 2011-01-01 false Basis of allocation between entities. Sec... AIR CARRIERS General Accounting Provisions Sec. 2-2 Basis of allocation between entities. (a) The... the air carrier, as well as each transport entity and organizational division of the air carrier for...

76 FR 52319 - Federal Advisory Committee Meeting Notice; Threat Reduction Advisory Committee

Federal Register 2010, 2011, 2012, 2013, 2014

2011-08-22

... DEPARTMENT OF DEFENSE Federal Advisory Committee Meeting Notice; Threat Reduction Advisory... committee meeting of the Threat Reduction Advisory Committee (Hereafter referred to as ``the Committee... CONTACT: Mr. William Hostyn, Defense Threat Reduction Agency/SP-ACP, 8725 John J. Kingman Road, MS 6201...

Functional diversification of Arabidopsis SEC1-related SM proteins in cytokinetic and secretory membrane fusion.

PubMed

Karnahl, Matthias; Park, Misoon; Krause, Cornelia; Hiller, Ulrike; Mayer, Ulrike; Stierhof, York-Dieter; Jürgens, Gerd

2018-06-12

Sec1/Munc18 (SM) proteins contribute to membrane fusion by interacting with Qa-SNAREs or nascent trans -SNARE complexes. Gymnosperms and the basal angiosperm Amborella have only a single SEC1 gene related to the KEULE gene in Arabidopsis However, the genomes of most angiosperms including Arabidopsis encode three SEC1-related SM proteins of which only KEULE has been functionally characterized as interacting with the cytokinesis-specific Qa-SNARE KNOLLE during cell-plate formation. Here we analyze the closest paralog of KEULE named SEC1B. In contrast to the cytokinesis defects of keule mutants, sec1b mutants are homozygous viable. However, the keule sec1b double mutant was nearly gametophytically lethal, displaying collapsed pollen grains, which suggests substantial overlap between SEC1B and KEULE functions in secretion-dependent growth. SEC1B had a strong preference for interaction with the evolutionarily ancient Qa-SNARE SYP132 involved in secretion and cytokinesis, whereas KEULE interacted with both KNOLLE and SYP132. This differential interaction with Qa-SNAREs is likely conferred by domains 1 and 2a of the two SM proteins. Comparative analysis of all four possible combinations of the relevant SEC1 Qa-SNARE double mutants revealed that in cytokinesis, the interaction of SEC1B with KNOLLE plays no role, whereas the interaction of KEULE with KNOLLE is prevalent and functionally as important as the interactions of both SEC1B and KEU with SYP132 together. Our results suggest that functional diversification of the two SEC1-related SM proteins during angiosperm evolution resulted in enhanced interaction of SEC1B with Qa-SNARE SYP132, and thus a predominant role of SEC1B in secretion.

Exocyst SEC3 and Phosphoinositides Define Sites of Exocytosis in Pollen Tube Initiation and Growth.

PubMed

Bloch, Daria; Pleskot, Roman; Pejchar, Přemysl; Potocký, Martin; Trpkošová, Pavlína; Cwiklik, Lukasz; Vukašinović, Nemanja; Sternberg, Hasana; Yalovsky, Shaul; Žárský, Viktor

2016-10-01

Polarized exocytosis is critical for pollen tube growth, but its localization and function are still under debate. The exocyst vesicle-tethering complex functions in polarized exocytosis. Here, we show that a sec3a exocyst subunit null mutant cannot be transmitted through the male gametophyte due to a defect in pollen tube growth. The green fluorescent protein (GFP)-SEC3a fusion protein is functional and accumulates at or proximal to the pollen tube tip plasma membrane. Partial complementation of sec3a resulted in the development of pollen with multiple tips, indicating that SEC3 is required to determine the site of pollen germination pore formation. Time-lapse imaging demonstrated that SEC3a and SEC8 were highly dynamic and that SEC3a localization on the apical plasma membrane predicts the direction of growth. At the tip, polar SEC3a domains coincided with cell wall deposition. Labeling of GFP-SEC3a-expressing pollen with the endocytic marker FM4-64 revealed the presence of subdomains on the apical membrane characterized by extensive exocytosis. In steady-state growing tobacco (Nicotiana tabacum) pollen tubes, SEC3a displayed amino-terminal Pleckstrin homology-like domain (SEC3a-N)-dependent subapical membrane localization. In agreement, SEC3a-N interacted with phosphoinositides in vitro and colocalized with a phosphatidylinositol 4,5-bisphosphate (PIP 2 ) marker in pollen tubes. Correspondingly, molecular dynamics simulations indicated that SEC3a-N associates with the membrane by interacting with PIP 2 However, the interaction with PIP 2 is not required for polar localization and the function of SEC3a in Arabidopsis (Arabidopsis thaliana). Taken together, our findings indicate that SEC3a is a critical determinant of polar exocytosis during tip growth and suggest differential regulation of the exocytotic machinery depending on pollen tube growth modes. © 2016 American Society of Plant Biologists. All Rights Reserved.

77 FR 12841 - The President's Management Advisory Board (PMAB); Notification of Upcoming Public Advisory Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-02

... recommendations aimed at improving Information Technology (IT) portfolio and project management, IT vendor... President's Management Advisory Board (PMAB); Notification of Upcoming Public Advisory Meeting AGENCY...: The President's Management Advisory Board (PMAB), a Federal Advisory Committee established in...

76 FR 65511 - The President's Management Advisory Board (PMAB); Notification of Upcoming Public Advisory Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-21

... management and operation, with a particular focus on productivity and the application of technology. Agenda... President's Management Advisory Board (PMAB); Notification of Upcoming Public Advisory Meeting AGENCY...: The President's Management Advisory Board (PMAB), a Federal Advisory Committee established in...

The Payload Advisory Panel and the Data and Information System Advisory Panel of the Investigators Working Group of the Earth Observing System: A joint report

NASA Technical Reports Server (NTRS)

Moore, Berrien, III; Dozier, Jeff; Barron, Eric J.; Batista, Getulio; Brewer, Peter; Grose, William; Harris, Graham; Hartmann, Dennis; Lau, William; Lemarshall, John

1993-01-01

The Payload Advisory Panel of the Investigators Working Group (IWG) for the Earth Observing System (EOS) met 4 to 6 October 1993 in Herndon, Virginia. The Panel, originally composed of the Interdisciplinary Science Principal Investigators, was expanded to include all Principal Investigators and as such is now the IWG itself. The meeting also addressed directly a report from the EOS Data and Information System (EOSDIS) Advisory Panel. The meeting focused on payload issues in the years 2000 to 2005; however, some subjects in the nearer-term, most significantly EOSDIS, were considered. The overarching theme of convergence in Earth observations set a backdrop for the entire meeting. Other themes included: atmospheric chemistry; remote sensing of the global cycles of energy, water, and carbon in EOS; ocean and land-ice altimetry; and the EOSDIS. The Totol Solar Irradiance Monitoring Report and results from the Accelerated Canopy Chemistry Program are included as appendices.

Polarized Exocytosis Induces Compensatory Endocytosis by Sec4p-Regulated Cortical Actin Polymerization

PubMed Central

Johansen, Jesper; Alfaro, Gabriel; Beh, Christopher T.

2016-01-01

Polarized growth is maintained by both polarized exocytosis, which transports membrane components to specific locations on the cell cortex, and endocytosis, which retrieves these components before they can diffuse away. Despite functional links between these two transport pathways, they are generally considered to be separate events. Using live cell imaging, in vivo and in vitro protein binding assays, and in vitro pyrene-actin polymerization assays, we show that the yeast Rab GTPase Sec4p couples polarized exocytosis with cortical actin polymerization, which induces endocytosis. After polarized exocytosis to the plasma membrane, Sec4p binds Las17/Bee1p (yeast Wiskott—Aldrich Syndrome protein [WASp]) in a complex with Sla1p and Sla2p during actin patch assembly. Mutations that inactivate Sec4p, or its guanine nucleotide exchange factor (GEF) Sec2p, inhibit actin patch formation, whereas the activating sec4-Q79L mutation accelerates patch assembly. In vitro assays of Arp2/3-dependent actin polymerization established that GTPγS-Sec4p overrides Sla1p inhibition of Las17p-dependent actin nucleation. These results support a model in which Sec4p relocates along the plasma membrane from polarized sites of exocytic vesicle fusion to nascent sites of endocytosis. Activated Sec4p then promotes actin polymerization and triggers compensatory endocytosis, which controls surface expansion and kinetically refines cell polarization. PMID:27526190

78 FR 40144 - Governmentwide Travel Advisory Committee (GTAC); Upcoming Public Advisory Committee Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2013-07-03

...] Governmentwide Travel Advisory Committee (GTAC); Upcoming Public Advisory Committee Meeting AGENCY: Office of Governmentwide Policy, General Services Administration (GSA). ACTION: Notice. SUMMARY: The Governmentwide Travel... Officer (DFO), Governmentwide Travel Advisory Committee (GTAC), Office of Governmentwide Policy, General...

77 FR 38065 - The President's Management Advisory Board (PMAB); Notification of Upcoming Public Advisory Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-26

... President's Management Advisory Board (PMAB); Notification of Upcoming Public Advisory Meeting AGENCY...: The President's Management Advisory Board (PMAB), a Federal Advisory Committee established in...: The meeting will be held on Wednesday, July 11, 2012, beginning at 9 a.m. eastern time, ending no...

The mechanoreceptors of the costo-vertebral joints

PubMed Central

Godwin-Austen, R. B.

1969-01-01

1. Unitary recording in the thoracic dorsal roots of mechanoreceptor discharges from the costo-vertebral joints was carried out in the cat and rabbit. Criteria for the identification of costo-vertebral joint mechanoreceptors were established. 2. The majority of rib joint mechanoreceptors are slowly adapting and fifty-three such receptors were studied. Five rapidly adapting receptors were also identified. 3. The responses of these receptors have been correlated with rib position and movement. The slowly adapting receptors gave a monotonic response to different rib positions. 72% showed an increase of discharge rate with displacements towards expiratory rib positions. 4. In response to manually imposed rib movements slowly adapting joint mechanoreceptors gave a dynamic response which was directly related to the velocity of the movement and adapted within 2 sec. The movements of breathing produced a corresponding alteration of the discharge frequency of the slowly adapting receptors. 5. Slowly adapting receptors were localized to the capsule of the costo-transverse joint by probing. They responded to increased intra-articular pressure with an increase of discharge rate and were silenced by intra-articular lignocaine, 0·4%. 6. Rapidly adapting joint mechanoreceptors responded to rib movement with a brief burst of discharges. 7. The rib joint mechanoreceptors signal rib joint position, and the direction and velocity of rib movement. It is suggested that these afferent discharges provide the basis for the perception of respiratory movements of the chest. The significance of these receptors to the `sense of effort' resulting from a resistance to breathing is discussed. PMID:5789947

75 FR 81245 - Federal Advisory Committee; Defense Intelligence Agency Advisory Board; Closed Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2010-12-27

... DEPARTMENT OF DEFENSE Office of the Secretary Federal Advisory Committee; Defense Intelligence Agency Advisory Board; Closed Meeting AGENCY: Defense Intelligence Agency, DoD. ACTION: Notice. SUMMARY... Defense announces that Defense Intelligence Agency Advisory Board and two of its subcommittees will meet...

75 FR 24926 - Federal Advisory Committee; Defense Intelligence Agency Advisory Board; Closed Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2010-05-06

... DEPARTMENT OF DEFENSE Office of the Secretary Federal Advisory Committee; Defense Intelligence Agency Advisory Board; Closed Meeting AGENCY: Defense Intelligence Agency, DoD. ACTION: Meeting notice... Department of Defense announces that Defense Intelligence Agency Advisory Board, and its subcommittees, will...

76 FR 41220 - Federal Advisory Committee; Defense Intelligence Agency Advisory Board; Closed Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2011-07-13

... DEPARTMENT OF DEFENSE Office of the Secretary Federal Advisory Committee; Defense Intelligence Agency Advisory Board; Closed Meeting AGENCY: Defense Intelligence Agency, DoD. ACTION: Meeting notice... Department of Defense announces that Defense Intelligence Agency Advisory Board and two of its subcommittees...

78 FR 8202 - Meeting of the Joint ACRS Subcommittees on Thermal Hydraulic Phenomena and Materials, Metallurgy...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-02-05

... NUCLEAR REGULATORY COMMISSION Advisory Committee on Reactor Safeguards (ACRS) Meeting of the Joint ACRS Subcommittees on Thermal Hydraulic Phenomena and Materials, Metallurgy and Reactor Fuels; Notice... Reactor Fuels will hold a meeting on February 20, 2013, Room T-2B1, 11545 Rockville Pike, Rockville...

78 FR 60863 - Threat Reduction Advisory Committee; Notice of Federal Advisory Committee Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2013-10-02

... DEPARTMENT OF DEFENSE Office of the Secretary Threat Reduction Advisory Committee; Notice of... Defense announces the following Federal advisory committee meeting of the Threat Reduction Advisory... Springs, CO. FOR FURTHER INFORMATION CONTACT: Mr. William Hostyn, DoD, Defense Threat Reduction Agency/J2...

46 CFR Sec. 20 - Reports of awards.

Code of Federal Regulations, 2011 CFR

2011-10-01

... REPAIRS UNDER NATIONAL SHIPPING AUTHORITY MASTER LUMP SUM REPAIR CONTRACT-NSA-LUMPSUMREP Sec. 20 Reports... submitted by the General Agents pursuant to section 3(d) of NSA Order 34 (SRM-3, Revised). ...

46 CFR Sec. 20 - Reports of awards.

Code of Federal Regulations, 2014 CFR

2014-10-01

... REPAIRS UNDER NATIONAL SHIPPING AUTHORITY MASTER LUMP SUM REPAIR CONTRACT-NSA-LUMPSUMREP Sec. 20 Reports... submitted by the General Agents pursuant to section 3(d) of NSA Order 34 (SRM-3, Revised). ...

46 CFR Sec. 20 - Reports of awards.

Code of Federal Regulations, 2012 CFR

2012-10-01

... REPAIRS UNDER NATIONAL SHIPPING AUTHORITY MASTER LUMP SUM REPAIR CONTRACT-NSA-LUMPSUMREP Sec. 20 Reports... submitted by the General Agents pursuant to section 3(d) of NSA Order 34 (SRM-3, Revised). ...

46 CFR Sec. 20 - Reports of awards.

Code of Federal Regulations, 2010 CFR

2010-10-01

... REPAIRS UNDER NATIONAL SHIPPING AUTHORITY MASTER LUMP SUM REPAIR CONTRACT-NSA-LUMPSUMREP Sec. 20 Reports... submitted by the General Agents pursuant to section 3(d) of NSA Order 34 (SRM-3, Revised). ...

46 CFR Sec. 20 - Reports of awards.

Code of Federal Regulations, 2013 CFR

2013-10-01

... REPAIRS UNDER NATIONAL SHIPPING AUTHORITY MASTER LUMP SUM REPAIR CONTRACT-NSA-LUMPSUMREP Sec. 20 Reports... submitted by the General Agents pursuant to section 3(d) of NSA Order 34 (SRM-3, Revised). ...

78 FR 29334 - Threat Reduction Advisory Committee; Notice of Federal Advisory Committee Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2013-05-20

... DEPARTMENT OF DEFENSE Office of the Secretary Threat Reduction Advisory Committee; Notice of... Defense announces the following Federal advisory committee meeting of the Threat Reduction Advisory..., CO. FOR FURTHER INFORMATION CONTACT: Mr. William Hostyn, DoD, Defense Threat Reduction Agency/J2/5/8R...

77 FR 2710 - Threat Reduction Advisory Committee; Notice of Federal Advisory Committee Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2012-01-19

... DEPARTMENT OF DEFENSE Office of the Secretary Threat Reduction Advisory Committee; Notice of... following Federal advisory committee meeting of the Threat Reduction Advisory Committee (hereafter referred... CONTACT: Mr. William Hostyn, GS-15, DoD, Defense Threat Reduction Agency/SP-ACP, 8725 John J. Kingman Road...

14 CFR Sec. 1-5 - Records.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Records. Sec. 1-5 Section 1-5 Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) ECONOMIC... information as will render certain the identification of all facts essential to a verification of the nature...

Dynamics and regulation of locomotion of a human swing leg as a double-pendulum considering self-impact joint constraint.

PubMed

Bazargan-Lari, Y; Eghtesad, M; Khoogar, A; Mohammad-Zadeh, A

2014-09-01

Despite some successful dynamic simulation of self-impact double pendulum (SIDP)-as humanoid robots legs or arms- studies, there is limited information available about the control of one leg locomotion. The main goal of this research is to improve the reliability of the mammalians leg locomotion and building more elaborated models close to the natural movements, by modeling the swing leg as a SIDP. This paper also presents the control design for a SIDP by a nonlinear model-based control method. To achieve this goal, the available data of normal human gait will be taken as the desired trajectories of the hip and knee joints. The model is characterized by the constraint that occurs at the knee joint (the lower joint of the model) in both dynamic modeling and control design. Since the system dynamics is nonlinear, the MIMO Input-Output Feedback Linearization method will be employed for control purposes. The first constraint in forward impact simulation happens at 0.5 rad where the speed of the upper link is increased to 2.5 rad/sec. and the speed of the lower link is reduced to -5 rad/sec. The subsequent constraints occur rather moderately. In the case of both backward and forward constraints simulation, the backward impact occurs at -0.5 rad and the speeds of the upper and lower links increase to 2.2 and 1.5 rad/sec., respectively. The designed controller performed suitably well and regulated the system accurately.

75 FR 37410 - Federal Advisory Committee; Defense Advisory Committee on Military Personnel Testing

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-29

... DEPARTMENT OF DEFENSE Office of the Secretary Federal Advisory Committee; Defense Advisory Committee on Military Personnel Testing AGENCY: Under Secretary of Defense for Personnel and Readiness, DoD...), and 41 CFR 102-3.150, DoD announces that the Defense Advisory Committee on Military Personnel Testing...

75 FR 63823 - Federal Advisory Committee; Defense Advisory Committee on Military Personnel Testing

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-18

... DEPARTMENT OF DEFENSE Office of the Secretary Federal Advisory Committee; Defense Advisory...), and 41 CFR 102-3.150, the Department of Defense announces that the Defense Advisory Committee on... OSD Federal Register Liaison Officer, Department of Defense. [FR Doc. 2010-26189 Filed 10-15-10; 8:45...

77 FR 19006 - Threat Reduction Advisory Committee; Notice of Federal Advisory Committee Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-29

... DEPARTMENT OF DEFENSE Office of the Secretary Threat Reduction Advisory Committee; Notice of... advisory committee meeting of the Threat Reduction Advisory Committee (hereafter referred to as ``the.... William Hostyn, GS-15, DoD, Defense Threat Reduction Agency/SP-ACP, 8725 John J. Kingman Road, MS 6201...

77 FR 50088 - Threat Reduction Advisory Committee; Notice of Federal Advisory Committee Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-20

... DEPARTMENT OF DEFENSE Office of the Secretary Threat Reduction Advisory Committee; Notice of... following Federal advisory committee meeting of the Threat Reduction Advisory Committee (hereafter referred... INFORMATION CONTACT: Mr. William Hostyn, GS-15, DoD, Defense Threat Reduction Agency/J2/5/8R, 8725 John J...

77 FR 69807 - Threat Reduction Advisory Committee; Notice of Federal Advisory Committee Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2012-11-21

... DEPARTMENT OF DEFENSE Office of the Secretary Threat Reduction Advisory Committee; Notice of... following Federal advisory committee meeting of the Threat Reduction Advisory Committee (hereafter referred... INFORMATION CONTACT: Mr. William Hostyn, DoD, Defense Threat Reduction Agency/J2/5/8R-ACP, 8725 John J...

46 CFR Sec. 6 - Awarding of work.

Code of Federal Regulations, 2012 CFR

2012-10-01

... REPAIRS UNDER NATIONAL SHIPPING AUTHORITY MASTER LUMP SUM REPAIR CONTRACT-NSA-LUMPSUMREP Sec. 6 Awarding... Repair Summary. (c) When an award is made, a job order in the form attached to the NSA-LUMPSUMREP...

46 CFR Sec. 6 - Awarding of work.

Code of Federal Regulations, 2014 CFR

2014-10-01

... REPAIRS UNDER NATIONAL SHIPPING AUTHORITY MASTER LUMP SUM REPAIR CONTRACT-NSA-LUMPSUMREP Sec. 6 Awarding... Repair Summary. (c) When an award is made, a job order in the form attached to the NSA-LUMPSUMREP...

46 CFR Sec. 6 - Awarding of work.

Code of Federal Regulations, 2013 CFR

2013-10-01

... REPAIRS UNDER NATIONAL SHIPPING AUTHORITY MASTER LUMP SUM REPAIR CONTRACT-NSA-LUMPSUMREP Sec. 6 Awarding... Repair Summary. (c) When an award is made, a job order in the form attached to the NSA-LUMPSUMREP...

76 FR 52016 - NASA International Space Station Advisory Committee and the Aerospace Safety Advisory Panel; Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2011-08-19

... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice (11-074)] NASA International Space Station Advisory Committee and the Aerospace Safety Advisory Panel; Meeting AGENCY: National Aeronautics and Space... meeting of the NASA International Space Station Advisory Committee and the Aerospace Safety Advisory Panel...

A "push and slide" mechanism allows sequence-insensitive translocation of secretory proteins by the SecA ATPase.

PubMed

Bauer, Benedikt W; Shemesh, Tom; Chen, Yu; Rapoport, Tom A

2014-06-05

In bacteria, most secretory proteins are translocated across the plasma membrane by the interplay of the SecA ATPase and the SecY channel. How SecA moves a broad range of polypeptide substrates is only poorly understood. Here we show that SecA moves polypeptides through the SecY channel by a "push and slide" mechanism. In its ATP-bound state, SecA interacts through a two-helix finger with a subset of amino acids in a substrate, pushing them into the channel. A polypeptide can also passively slide back and forth when SecA is in the predominant ADP-bound state or when SecA encounters a poorly interacting amino acid in its ATP-bound state. SecA performs multiple rounds of ATP hydrolysis before dissociating from SecY. The proposed push and slide mechanism is supported by a mathematical model and explains how SecA allows translocation of a wide range of polypeptides. This mechanism may also apply to hexameric polypeptide-translocating ATPases. Copyright © 2014 Elsevier Inc. All rights reserved.

78 FR 28622 - Notice of Public Meeting: Joint session of Northeast California Resource Advisory Council and...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-05-15

... welcome on the field tour. They must provide their own transportation, food and beverages. Individuals who... DEPARTMENT OF THE INTERIOR Bureau of Land Management [LLCAN01000.L18200000.XZ0000; 13-00160-ILM... California Resource Advisory Council, and Individual Council Meetings AGENCY: Bureau of Land Management...

46 CFR Sec. 4 - Method of payment.

Code of Federal Regulations, 2010 CFR

2010-10-01

... AGENTS IN PREPARATION OF INVOICES AND PAYMENT OF COMPENSATION PURSUANT TO PROVISIONS OF NSA ORDER NO. 47 Sec. 4 Method of payment. The General Agent shall prepare check drawn on the NSA Special bank account...

46 CFR Sec. 4 - Method of payment.

Code of Federal Regulations, 2014 CFR

2014-10-01

... AGENTS IN PREPARATION OF INVOICES AND PAYMENT OF COMPENSATION PURSUANT TO PROVISIONS OF NSA ORDER NO. 47 Sec. 4 Method of payment. The General Agent shall prepare check drawn on the NSA Special bank account...

46 CFR Sec. 4 - Method of payment.

Code of Federal Regulations, 2013 CFR

2013-10-01

... AGENTS IN PREPARATION OF INVOICES AND PAYMENT OF COMPENSATION PURSUANT TO PROVISIONS OF NSA ORDER NO. 47 Sec. 4 Method of payment. The General Agent shall prepare check drawn on the NSA Special bank account...

46 CFR Sec. 4 - Method of payment.

Code of Federal Regulations, 2012 CFR

2012-10-01

... AGENTS IN PREPARATION OF INVOICES AND PAYMENT OF COMPENSATION PURSUANT TO PROVISIONS OF NSA ORDER NO. 47 Sec. 4 Method of payment. The General Agent shall prepare check drawn on the NSA Special bank account...

77 FR 55863 - NASA Advisory Council; Science Committee; Earth Science Subcommittee; Applied Sciences Advisory...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-09-11

... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice (12-072)] NASA Advisory Council; Science Committee; Earth Science Subcommittee; Applied Sciences Advisory Group Meeting AGENCY: National Aeronautics... the Applied Science Advisory Group. This Subcommittee reports to the Earth Science Subcommittee...

46 CFR Sec. 3 - Master's requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 8 2010-10-01 2010-10-01 false Master's requirements. Sec. 3 Section 3 Shipping... Master's requirements. The Master shall: (a) Receive and receipt for the quantities of slop chest items... therefor, at the unit price, specified by the Slop Chest Statement furnished the Master by the General...

46 CFR Sec. 3 - Master's requirements.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 8 2011-10-01 2011-10-01 false Master's requirements. Sec. 3 Section 3 Shipping... Master's requirements. The Master shall: (a) Receive and receipt for the quantities of slop chest items... therefor, at the unit price, specified by the Slop Chest Statement furnished the Master by the General...

46 CFR Sec. 3 - Master's requirements.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 8 2012-10-01 2012-10-01 false Master's requirements. Sec. 3 Section 3 Shipping... Master's requirements. The Master shall: (a) Receive and receipt for the quantities of slop chest items... therefor, at the unit price, specified by the Slop Chest Statement furnished the Master by the General...

46 CFR Sec. 3 - Master's requirements.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 8 2014-10-01 2014-10-01 false Master's requirements. Sec. 3 Section 3 Shipping... Master's requirements. The Master shall: (a) Receive and receipt for the quantities of slop chest items... therefor, at the unit price, specified by the Slop Chest Statement furnished the Master by the General...

46 CFR Sec. 3 - Master's requirements.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 8 2013-10-01 2013-10-01 false Master's requirements. Sec. 3 Section 3 Shipping... Master's requirements. The Master shall: (a) Receive and receipt for the quantities of slop chest items... therefor, at the unit price, specified by the Slop Chest Statement furnished the Master by the General...

46 CFR Sec. 10 - Lost documents.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 8 2012-10-01 2012-10-01 false Lost documents. Sec. 10 Section 10 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION A-NATIONAL SHIPPING AUTHORITY PROCEDURAL RULES FOR FINANCIAL... certification shall be placed on such copies: I certify that, to the best of my knowledge and belief, this is a...

46 CFR Sec. 10 - Lost documents.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 8 2014-10-01 2014-10-01 false Lost documents. Sec. 10 Section 10 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION A-NATIONAL SHIPPING AUTHORITY PROCEDURAL RULES FOR FINANCIAL... certification shall be placed on such copies: I certify that, to the best of my knowledge and belief, this is a...

46 CFR Sec. 10 - Lost documents.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 8 2010-10-01 2010-10-01 false Lost documents. Sec. 10 Section 10 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION A-NATIONAL SHIPPING AUTHORITY PROCEDURAL RULES FOR FINANCIAL... certification shall be placed on such copies: I certify that, to the best of my knowledge and belief, this is a...

46 CFR Sec. 10 - Lost documents.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 8 2011-10-01 2011-10-01 false Lost documents. Sec. 10 Section 10 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION A-NATIONAL SHIPPING AUTHORITY PROCEDURAL RULES FOR FINANCIAL... certification shall be placed on such copies: I certify that, to the best of my knowledge and belief, this is a...

46 CFR Sec. 10 - Lost documents.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 8 2013-10-01 2013-10-01 false Lost documents. Sec. 10 Section 10 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION A-NATIONAL SHIPPING AUTHORITY PROCEDURAL RULES FOR FINANCIAL... certification shall be placed on such copies: I certify that, to the best of my knowledge and belief, this is a...

Recent developments and applications of the SEC vidicon for astronomy

NASA Technical Reports Server (NTRS)

Zucchino, P.; Lowrance, J. L.

1971-01-01

The engineering development of the SEC vidicon as an astronomical sensor has continued in parallel with its operational use. One scientific application was the six hour exposure of the quasar PHL-957 at the Coude spectrograph of the 200-inch Hale telescope. The developmental effort includes both the enhancement of the basic attributes that make the SEC an appropriate sensor, namely, high quantum efficiency, low threshold, and long integration; as well as work to broaden its scientific usefulness, such as the development of a MgF2 photocathode window for vacuum ultraviolet sensitivity, and a permanent magnet focus design for thermal compatibility with proposed large space telescopes. Additional details on the characteristics of the SEC tube are discussed, as well as plans to make a larger and higher resolution version.

SEC14 is a specific requirement for secretion of phospholipase B1 and pathogenicity of Cryptococcus neoformans

PubMed Central

Chayakulkeeree, Methee; Johnston, Simon Andrew; Oei, Johanes Bijosono; Lev, Sophie; Williamson, Peter Richard; Wilson, Christabel Frewen; Zuo, Xiaoming; Leal, Ana Lusia; Vainstein, Marilene Henning; Meyer, Wieland; Sorrell, Tania Christine; May, Robin Charles; Djordjevic, Julianne Teresa

2011-01-01

Summary Secreted phospholipase B1 (CnPlb1) is essential for dissemination of Cryptococcus neoformans to the central nervous system (CNS) yet essential components of its secretion machinery remain to be elucidated. Using gene deletion analysis we demonstrate that CnPlb1 secretion is dependent on the CnSEC14 product, CnSec14-1p. CnSec14-1p is a homologue of the phosphatidylinositol transfer protein (PITP) ScSec14p, which is essential for secretion and viability in Saccharomyces cerevisiae. In contrast to CnPlb1, neither laccase 1 (Lac1)-induced melanization within the cell wall nor capsule induction were negatively impacted in CnSEC14-1 deletion mutants (CnΔsec14-1 and CnΔsec14-1CnΔsfh5). Similar to the CnPLB1 deletion mutant (CnΔplb1), CnΔsec14-1 was hypo-virulent in mice and did not disseminate to the CNS by day 14 post infection. Furthermore, macrophage expulsion of live CnΔsec14-1 and CnΔplb1 (vomocytosis) was reduced. Individual deletion of CnSEC14-2, a closely-related CnSEC14-1 homologue, and CnSFH5, a distantly-related SEC fourteen-like homologue, did not abrogate CnPlb1 secretion or virulence. However, reconstitution of CnΔsec14-1 with CnSEC14-1 or CnSEC14-2 restored both phenotypes, consistent with functional genetic redundancy. We conclude that CnPlb1 secretion is SEC14-dependent and that C. neoformans preferentially exports virulence determinants to the cell periphery via distinct pathways. We also demonstrate that CnPlb1 secretion is essential for vomocytosis. PMID:21453402

77 FR 61581 - Notice of Advisory Committee Closed Meeting; U.S. Strategic Command Strategic Advisory Group

Federal Register 2010, 2011, 2012, 2013, 2014

2012-10-10

.... Strategic Command Strategic Advisory Group AGENCY: Department of Defense. ACTION: Notice of Advisory... advisory committee: U.S. Strategic Command Strategic Advisory Group. DATES: November 15, 2012, from 8 a.m... Command, during the development of the Nation's strategic war plans. Agenda: Topics include: Policy Issues...

78 FR 67131 - Notice of Advisory Committee Closed Meeting; U.S. Strategic Command Strategic Advisory Group

Federal Register 2010, 2011, 2012, 2013, 2014

2013-11-08

.... Strategic Command Strategic Advisory Group AGENCY: Department of Defense. ACTION: Notice of Advisory... following Federal Advisory Committee meeting of the U.S. Strategic Command Strategic Advisory Group. DATES... issues to the Commander, U.S. Strategic Command, during the development of the Nation's strategic war...

77 FR 46802 - National Emergency Medical Services Advisory Council (NEMSAC); Notice of Federal Advisory...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-06

...-0100] National Emergency Medical Services Advisory Council (NEMSAC); Notice of Federal Advisory... Transportation (DOT). ACTION: Meeting Notice--National Emergency Medical Services Advisory Council. SUMMARY: The... NEMSAC is to provide a nationally recognized council of emergency medical services representatives and...

78 FR 41937 - Joint Meeting of the Gastroenterology-Urology Panel and the Radiological Devices Panel of the...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-07-12

... DEPARTMENT OF HEALTH AND HUMAN SERVICES Food and Drug Administration [Docket No. FDA-2013-N-0816] Joint Meeting of the Gastroenterology-Urology Panel and the Radiological Devices Panel of the Medical...: Gastroenterology-Urology Panel and Radiological Devices Panel of the Medical Devices Advisory Committee. General...

78 FR 49332 - National Emergency Medical Services Advisory Council (NEMSAC); Notice of Federal Advisory...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-08-13

...-0091] National Emergency Medical Services Advisory Council (NEMSAC); Notice of Federal Advisory... Transportation (DOT). ACTION: Meeting Notice--National Emergency Medical Services Advisory Council. SUMMARY: The... emergency medical services representatives and consumers, is to advise and consult with DOT and the Federal...

75 FR 60484 - NASA Advisory Council; Science Committee; Earth Science Subcommittee; Applied Sciences Advisory...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-30

... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice: (10-115)] NASA Advisory Council; Science Committee; Earth Science Subcommittee; Applied Sciences Advisory Group Meeting AGENCY: National Aeronautics...) announces a meeting of the Applied Science Advisory Group. This Subcommittee reports to the Earth Science...

76 FR 60811 - Notice of Advisory Committee Closed Meeting; U.S. Strategic Command Strategic Advisory Group...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-09-30

... DEPARTMENT OF DEFENSE Notice of Advisory Committee Closed Meeting; U.S. Strategic Command Strategic Advisory Group; Correction AGENCY: Department of Defense. ACTION: Notice of Advisory Committee... Command Strategic Advisory Group gave notice of a meeting to be held on November 1, 2011, from 8 a.m. to 5...

75 FR 60430 - Federal Advisory Committee; Threat Reduction Advisory Committee

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-30

... DEPARTMENT OF DEFENSE Office of the Secretary Federal Advisory Committee; Threat Reduction... amended) the Department of Defense announces a meeting of the Threat Reduction Advisory Committee... Wright, Defense Threat Reduction Agency/AST, 8725 John J. Kingman Road, MS 6201, Fort Belvoir, VA 22060...

78 FR 77443 - Electricity Advisory Committee

Federal Register 2010, 2011, 2012, 2013, 2014

2013-12-23

... DEPARTMENT OF ENERGY Electricity Advisory Committee AGENCY: Office of Electricity Delivery and... announces a meeting of the Electricity Advisory Committee (EAC). The Federal Advisory Committee Act (Pub. L... at: http://energy.gov/oe/services/electricity-advisory-committee-eac . FOR FURTHER INFORMATION...

Exocyst SEC3 and Phosphoinositides Define Sites of Exocytosis in Pollen Tube Initiation and Growth1[OPEN

PubMed Central

Bloch, Daria; Pleskot, Roman; Vukašinović, Nemanja

2016-01-01

Polarized exocytosis is critical for pollen tube growth, but its localization and function are still under debate. The exocyst vesicle-tethering complex functions in polarized exocytosis. Here, we show that a sec3a exocyst subunit null mutant cannot be transmitted through the male gametophyte due to a defect in pollen tube growth. The green fluorescent protein (GFP)-SEC3a fusion protein is functional and accumulates at or proximal to the pollen tube tip plasma membrane. Partial complementation of sec3a resulted in the development of pollen with multiple tips, indicating that SEC3 is required to determine the site of pollen germination pore formation. Time-lapse imaging demonstrated that SEC3a and SEC8 were highly dynamic and that SEC3a localization on the apical plasma membrane predicts the direction of growth. At the tip, polar SEC3a domains coincided with cell wall deposition. Labeling of GFP-SEC3a-expressing pollen with the endocytic marker FM4-64 revealed the presence of subdomains on the apical membrane characterized by extensive exocytosis. In steady-state growing tobacco (Nicotiana tabacum) pollen tubes, SEC3a displayed amino-terminal Pleckstrin homology-like domain (SEC3a-N)-dependent subapical membrane localization. In agreement, SEC3a-N interacted with phosphoinositides in vitro and colocalized with a phosphatidylinositol 4,5-bisphosphate (PIP2) marker in pollen tubes. Correspondingly, molecular dynamics simulations indicated that SEC3a-N associates with the membrane by interacting with PIP2. However, the interaction with PIP2 is not required for polar localization and the function of SEC3a in Arabidopsis (Arabidopsis thaliana). Taken together, our findings indicate that SEC3a is a critical determinant of polar exocytosis during tip growth and suggest differential regulation of the exocytotic machinery depending on pollen tube growth modes. PMID:27516531

Future Research Opportunities in Peri-Prosthetic Joint Infection Prevention.

PubMed

Berbari, Elie; Segreti, John; Parvizi, Javad; Berríos-Torres, Sandra I

Peri-prosthetic joint infection (PJI) is a serious complication of prosthetic joint arthroplasty. A better understanding and reversal of modifiable risk factors may lead to a reduction in the incidence of incisional (superficial and deep) and organ/space (e.g., PJI) surgical site infections (SSI). Recently, the Centers for Disease Control and Prevention (CDC) and the Healthcare Infection Control Practices Advisory Committee (HICPAC) published the Guideline for Prevention of Surgical Site Infection. This targeted update applies evidence-based methodology in drafting recommendations for potential strategies to reduce the risk of SSI both across surgical procedures and specifically in prosthetic joint arthroplasty. A panel of PJI content experts identified nine PJI prevention research opportunities based on both evidence gaps identified through the guideline development process (transfusion, immunosuppressive therapy, anticoagulation, orthopedic space suit, and biofilm) and expert opinion (anesthesia, operative room environment, glycemic control, and Staphylococcus aureus nasal screening and decolonization. This article offers a road map for PJI prevention research.

Mechanisms of ribosome stalling by SecM at multiple elongation steps

PubMed Central

Zhang, Jun; Pan, Xijiang; Yan, Kaige; Sun, Shan; Gao, Ning; Sui, Sen-Fang

2015-01-01

Regulation of translating ribosomes is a major component of gene expression control network. In Escherichia coli, ribosome stalling by the C-terminal arrest sequence of SecM regulates the SecA-dependent secretion pathway. Previous studies reported many residues of SecM peptide and ribosome exit tunnel are critical for stalling. However, the underlying molecular mechanism is still not clear at the atomic level. Here, we present two cryo-EM structures of the SecM-stalled ribosomes at 3.3–3.7 Å resolution, which reveal two different stalling mechanisms at distinct elongation steps of the translation cycle: one is due to the inactivation of ribosomal peptidyl-transferase center which inhibits peptide bond formation with the incoming prolyl-tRNA; the other is the prolonged residence of the peptidyl-RNA at the hybrid A/P site which inhibits the full-scale tRNA translocation. These results demonstrate an elegant control of translation cycle by regulatory peptides through a continuous, dynamic reshaping of the functional center of the ribosome. DOI: http://dx.doi.org/10.7554/eLife.09684.001 PMID:26670735

17 CFR 275.203A-1 - Eligibility for SEC registration; Switching to or from SEC registration.

Code of Federal Regulations, 2013 CFR

2013-04-01

.... This paragraph (a) does not apply if: (i) You are an investment adviser to an investment company... under sections 203(l) or 203(m) of the Act (15 U.S.C. 80b-3(l), (m)). (2) SEC-registered advisers... an exemption from registration under sections 203(l) or 203(m) of the Act (15 U.S.C. 80b-3(l), (m...

17 CFR 275.203A-1 - Eligibility for SEC registration; Switching to or from SEC registration.

Code of Federal Regulations, 2014 CFR

2014-04-01

.... This paragraph (a) does not apply if: (i) You are an investment adviser to an investment company... under sections 203(l) or 203(m) of the Act (15 U.S.C. 80b-3(l), (m)). (2) SEC-registered advisers... an exemption from registration under sections 203(l) or 203(m) of the Act (15 U.S.C. 80b-3(l), (m...

75 FR 32469 - Consumer Advisory Council; Notice of Meeting of the Consumer Advisory Council

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-08

... FEDERAL RESERVE SYSTEM Consumer Advisory Council; Notice of Meeting of the Consumer Advisory Council The Consumer Advisory Council will meet on Thursday, June 17, 2010. The meeting, which will be...'s responsibilities under various consumer financial services laws and on other matters on which the...

Exocyst sec5 regulates exocytosis of newcomer insulin granules underlying biphasic insulin secretion.

PubMed

Xie, Li; Zhu, Dan; Kang, Youhou; Liang, Tao; He, Yu; Gaisano, Herbert Y

2013-01-01

The exocyst complex subunit Sec5 is a downstream effector of RalA-GTPase which promotes RalA-exocyst interactions and exocyst assembly, serving to tether secretory granules to docking sites on the plasma membrane. We recently reported that RalA regulates biphasic insulin secretion in pancreatic islet β cells in part by tethering insulin secretory granules to Ca(2+) channels to assist excitosome assembly. Here, we assessed β cell exocytosis by patch clamp membrane capacitance measurement and total internal reflection fluorescence microscopy to investigate the role of Sec5 in regulating insulin secretion. Sec5 is present in human and rodent islet β cells, localized to insulin granules. Sec5 protein depletion in rat INS-1 cells inhibited depolarization-induced release of primed insulin granules from both readily-releasable pool and mobilization from the reserve pool. This reduction in insulin exocytosis was attributed mainly to reduction in recruitment and exocytosis of newcomer insulin granules that undergo minimal docking time at the plasma membrane, but which encompassed a larger portion of biphasic glucose stimulated insulin secretion. Sec5 protein knockdown had little effect on predocked granules, unless vigorously stimulated by KCl depolarization. Taken together, newcomer insulin granules in β cells are more sensitive than predocked granules to Sec5 regulation.

16 CFR 1018.26 - Advisory functions.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 16 Commercial Practices 2 2010-01-01 2010-01-01 false Advisory functions. 1018.26 Section 1018.26 Commercial Practices CONSUMER PRODUCT SAFETY COMMISSION GENERAL ADVISORY COMMITTEE MANAGEMENT Operation of Advisory Committees § 1018.26 Advisory functions. (a) Unless otherwise specifically provided by statute...

76 FR 29752 - The President's Management Advisory Board (PMAB); Notification of Upcoming Public Advisory Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2011-05-23

...The President's Management Advisory Board, a Federal Advisory Committee established in accordance with the Federal Advisory Committee Act (FACA), 5 U.S.C., App., and Executive Order 13538, will hold a public meeting on June 17, 2011.

75 FR 27557 - Federal Advisory Committee Act

Federal Register 2010, 2011, 2012, 2013, 2014

2010-05-17

... the charter for the Advisory Committee for the 2012 Radiocommunication Conference (WRC-12 Advisory Committee), for a two-year period. The WRC-12 Advisory Committee is a Federal advisory committee under the... CONTACT: Alexander Roytblat, Designated Federal Official, WRC-12 Advisory Committee, FCC International...

46 CFR Sec. 5 - Responsibility for duplicating copies of NSA-WORKSMALREP Contract.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 8 2014-10-01 2014-10-01 false Responsibility for duplicating copies of NSA-WORKSMALREP Contract. Sec. 5 Section 5 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION A-NATIONAL... INDIVIDUAL CONTRACT FOR MINOR REPAIRS-NSA-WORKSMALREP Sec. 5 Responsibility for duplicating copies of NSA...

46 CFR Sec. 5 - Responsibility for duplicating copies of NSA-WORKSMALREP Contract.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 8 2013-10-01 2013-10-01 false Responsibility for duplicating copies of NSA-WORKSMALREP Contract. Sec. 5 Section 5 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION A-NATIONAL... INDIVIDUAL CONTRACT FOR MINOR REPAIRS-NSA-WORKSMALREP Sec. 5 Responsibility for duplicating copies of NSA...

46 CFR Sec. 5 - Responsibility for duplicating copies of NSA-WORKSMALREP Contract.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 8 2011-10-01 2011-10-01 false Responsibility for duplicating copies of NSA-WORKSMALREP Contract. Sec. 5 Section 5 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION A-NATIONAL... INDIVIDUAL CONTRACT FOR MINOR REPAIRS-NSA-WORKSMALREP Sec. 5 Responsibility for duplicating copies of NSA...

46 CFR Sec. 5 - Responsibility for duplicating copies of NSA-WORKSMALREP Contract.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 8 2012-10-01 2012-10-01 false Responsibility for duplicating copies of NSA-WORKSMALREP Contract. Sec. 5 Section 5 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION A-NATIONAL... INDIVIDUAL CONTRACT FOR MINOR REPAIRS-NSA-WORKSMALREP Sec. 5 Responsibility for duplicating copies of NSA...

46 CFR Sec. 5 - Responsibility for duplicating copies of NSA-WORKSMALREP Contract.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 8 2010-10-01 2010-10-01 false Responsibility for duplicating copies of NSA-WORKSMALREP Contract. Sec. 5 Section 5 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION A-NATIONAL... INDIVIDUAL CONTRACT FOR MINOR REPAIRS-NSA-WORKSMALREP Sec. 5 Responsibility for duplicating copies of NSA...

CHARACTERIZATION OF HUMIC ACID SIZE FRACTIONS BY SEC AND MALS (R822832)

EPA Science Inventory

Latahco silt-loam humic acid was separated on a preparatory scale by size exclusion chromatography (SEC) on a gravity-fed Sepharose column. Four fractions from this separation were collected and further analyzed, along with whole humic acid, by high-performance SEC coupled with a...

14 CFR Sec. 19-4 - Service classes.

Code of Federal Regulations, 2010 CFR

2010-01-01

... a composite of first class, coach, and mixed passenger/cargo service. The following classifications... integral part of services performed pursuant to published flight schedules. The following classifications... Classifications Sec. 19-4 Service classes. The statistical classifications are designed to reflect the operating...

14 CFR Sec. 19-4 - Service classes.

Code of Federal Regulations, 2011 CFR

2011-01-01

... a composite of first class, coach, and mixed passenger/cargo service. The following classifications... integral part of services performed pursuant to published flight schedules. The following classifications... Classifications Sec. 19-4 Service classes. The statistical classifications are designed to reflect the operating...

The 12 Item Social and Economic Conservatism Scale (SECS)

PubMed Central

Everett, Jim A. C.

2013-01-01

Recent years have seen a surge in psychological research on the relationship between political ideology (particularly conservatism) and cognition, affect, behaviour, and even biology. Despite this flurry of investigation, however, there is as yet no accepted, validated, and widely used multi-item scale of conservatism that is concise, that is modern in its conceptualisation, and that includes both social and economic conservatism subscales. In this paper the 12-Item Social and Economic Conservatism Scale (SECS) is proposed and validated to help fill this gap. The SECS is suggested to be an important and useful tool for researchers working in political psychology. PMID:24349200

The 12 item Social and Economic Conservatism Scale (SECS).

PubMed

Everett, Jim A C

2013-01-01

Recent years have seen a surge in psychological research on the relationship between political ideology (particularly conservatism) and cognition, affect, behaviour, and even biology. Despite this flurry of investigation, however, there is as yet no accepted, validated, and widely used multi-item scale of conservatism that is concise, that is modern in its conceptualisation, and that includes both social and economic conservatism subscales. In this paper the 12-Item Social and Economic Conservatism Scale (SECS) is proposed and validated to help fill this gap. The SECS is suggested to be an important and useful tool for researchers working in political psychology.

75 FR 43493 - Office of the Secretary; Federal Advisory Committee; Defense Intelligence Agency Advisory Board...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-07-26

... DEPARTMENT OF DEFENSE Office of the Secretary; Federal Advisory Committee; Defense Intelligence Agency Advisory Board; Closed Meeting AGENCY: Defense Intelligence Agency, DOD. ACTION: Meeting notice... Department of Defense announces that Defense Intelligence Agency Advisory Board will meet on September 1 and...

75 FR 13555 - Compliance Policy Guide Sec. 540.375 Canned Salmon - Adulteration Involving Decomposition (CPG...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-22

...] (Formerly Docket No. 1998N-0046) Compliance Policy Guide Sec. 540.375 Canned Salmon -- Adulteration... of Compliance Policy Guide Sec. 540.375 Canned Salmon -- Adulteration Involving Decomposition (CPG... relating to decomposition in fish and fishery products, including canned salmon, is provided in CPG Sec...

Efficient secretion of small proteins in mammalian cells relies on Sec62-dependent posttranslational translocation

PubMed Central

Lakkaraju, Asvin K. K.; Thankappan, Ratheeshkumar; Mary, Camille; Garrison, Jennifer L.; Taunton, Jack; Strub, Katharina

2012-01-01

Mammalian cells secrete a large number of small proteins, but their mode of translocation into the endoplasmic reticulum is not fully understood. Cotranslational translocation was expected to be inefficient due to the small time window for signal sequence recognition by the signal recognition particle (SRP). Impairing the SRP pathway and reducing cellular levels of the translocon component Sec62 by RNA interference, we found an alternate, Sec62-dependent translocation path in mammalian cells required for the efficient translocation of small proteins with N-terminal signal sequences. The Sec62-dependent translocation occurs posttranslationally via the Sec61 translocon and requires ATP. We classified preproteins into three groups: 1) those that comprise ≤100 amino acids are strongly dependent on Sec62 for efficient translocation; 2) those in the size range of 120–160 amino acids use the SRP pathway, albeit inefficiently, and therefore rely on Sec62 for efficient translocation; and 3) those larger than 160 amino acids depend on the SRP pathway to preserve a transient translocation competence independent of Sec62. Thus, unlike in yeast, the Sec62-dependent translocation pathway in mammalian cells serves mainly as a fail-safe mechanism to ensure efficient secretion of small proteins and provides cells with an opportunity to regulate secretion of small proteins independent of the SRP pathway. PMID:22648169

Identification of protein components and quantitative immunoassay for SEC2 in staphylococcin injection.

PubMed

Ding, Ding; Huang, Peng; Sun, Hongying; Xue, Qiao; Pan, Yingqiu; Chen, Shuqing

2009-08-15

In China, staphylococcin injection has been commonly used in combined cancer therapy to enhance the systemic immune response and reduce the toxicities associated with chemotherapy or radiation therapy in the last decade. It is claimed that the main effective component is staphylococcal enterotoxin C2 (SEC2). However, no standard method based on the concentration of SEC2 has been established for quality control of the injection products. In this study, a sensitive and reliable biotin-streptavidin-ELISA (BS-ELISA) method was established for detection and quantification of SEC2. In addition, 1-D SDS-PAGE coupled with nano-LC-MS/MS was performed to identify the protein components in the injection products from one manufacturing company. The results of the BS-ELISA showed that SEC2 only accounted for less than 0.1% of the total protein in the injection products, and the nano-LC-MS/MS results showed that fifty-five proteins of Staphylococcus aureus were confidently identified in the injection solution. Seventeen out of these proteins, including SEC2, were well-known virulence factors. In addition, eighteen proteins of other Gram-positive bacteria were also confidently identified. Thus, the results indicated that SEC2 is of very low concentration in the injection products and the process of the injection preparation should be improved for health and safety consideration.

Characterization of a gene product (Sec53p) required for protein assembly in the yeast endoplasmic reticulum

PubMed Central

1985-01-01

SEC53, a gene that is required for completion of assembly of proteins in the endoplasmic reticulum in yeast, has been cloned, sequenced, and the product localized by cell fractionation. Complementation of a sec53 mutation is achieved with unique plasmids from genomic or cDNA expression banks. These inserts contain the authentic gene, a cloned copy of which integrates at the sec53 locus. An open reading frame in the insert predicts a 29-kD protein with no significant hydrophobic character. This prediction is confirmed by detection of a 28-kD protein overproduced in cells that carry SEC53 on a multicopy plasmid. To follow Sec53p more directly, a LacZ-SEC53 gene fusion has been constructed which allows the isolation of a hybrid protein for use in production of antibody. With such an antibody, quantitative immune decoration has shown that the sec53-6 mutation decreases the level of Sec53p at 37 degrees C, while levels comparable to wild-type are seen at 24 degrees C. An eightfold overproduction of Sec53p accompanies transformation of cells with a multicopy plasmid containing SEC53. Cell fractionation, performed with conditions that preserve the lumenal content of the endoplasmic reticulum (ER), shows Sec53p highly enriched in the cytosol fraction. We suggest that Sec53p acts indirectly to facilitate assembly in the ER, possibly by interacting with a stable ER component, or by providing a small molecule, other than an oligosaccharide precursor, necessary for the assembly event. PMID:3905826

75 FR 18824 - Federal Advisory Committee; Meeting of the Department of Defense Historical Advisory Committee

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-13

... DEPARTMENT OF DEFENSE Office of the Secretary Federal Advisory Committee; Meeting of the Department of Defense Historical Advisory Committee AGENCY: Department of Defense (DoD). ACTION: Notice of... notice announces a meeting of the Department of Defense Historical Advisory Committee. During the meeting...

78 FR 21349 - Defense Advisory Committee on Military Personnel Testing; Notice of Federal Advisory Committee...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-04-10

... DEPARTMENT OF DEFENSE Office of the Secretary Defense Advisory Committee on Military Personnel... advisory committee meeting of the Defense Advisory Committee on Military Personnel Testing will take place... meeting is to review planned changes and progress in developing computerized tests for military enlistment...

Novel Allylic Oxidation of α-Cedrene to sec-Cedrenol by a Rhodococcus Strain

PubMed Central

Takigawa, Hirofumi; Kubota, Hiromi; Sonohara, Hiroshi; Okuda, Mitsuyoshi; Tanaka, Shigeyoshi; Fujikura, Yoshiaki; Ito, Susumu

1993-01-01

A bacterial strain, designated KSM-7358, that can use α-cedrene for growth was isolated. The strain was identified as a member of the genus Rhodococcus and catalyzed the novel allylic oxidation of α-cedrene regiospecifically to produce (R)-10-hydroxycedrene (sec-cedrenol) with a very high yield. α-Curcumene was also produced as a possible metabolite of sec-cedrenol. A possible pathway for the microbial conversion of α-cedrene to sec-cedrenol and α-curcumene is proposed. PMID:16348930

46 CFR Sec. 19 - Ship Repair Summaries.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 8 2012-10-01 2012-10-01 false Ship Repair Summaries. Sec. 19 Section 19 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION A-NATIONAL SHIPPING AUTHORITY PROCEDURE FOR ACCOMPLISHMENT OF VESSEL REPAIRS UNDER NATIONAL SHIPPING AUTHORITY MASTER LUMP SUM REPAIR CONTRACT-NSA-LUMPSUMREP...

46 CFR Sec. 19 - Ship Repair Summaries.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 8 2011-10-01 2011-10-01 false Ship Repair Summaries. Sec. 19 Section 19 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION A-NATIONAL SHIPPING AUTHORITY PROCEDURE FOR ACCOMPLISHMENT OF VESSEL REPAIRS UNDER NATIONAL SHIPPING AUTHORITY MASTER LUMP SUM REPAIR CONTRACT-NSA-LUMPSUMREP...

46 CFR Sec. 19 - Ship Repair Summaries.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 8 2010-10-01 2010-10-01 false Ship Repair Summaries. Sec. 19 Section 19 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION A-NATIONAL SHIPPING AUTHORITY PROCEDURE FOR ACCOMPLISHMENT OF VESSEL REPAIRS UNDER NATIONAL SHIPPING AUTHORITY MASTER LUMP SUM REPAIR CONTRACT-NSA-LUMPSUMREP...

46 CFR Sec. 19 - Ship Repair Summaries.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 8 2013-10-01 2013-10-01 false Ship Repair Summaries. Sec. 19 Section 19 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION A-NATIONAL SHIPPING AUTHORITY PROCEDURE FOR ACCOMPLISHMENT OF VESSEL REPAIRS UNDER NATIONAL SHIPPING AUTHORITY MASTER LUMP SUM REPAIR CONTRACT-NSA-LUMPSUMREP...

46 CFR Sec. 19 - Ship Repair Summaries.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 8 2014-10-01 2014-10-01 false Ship Repair Summaries. Sec. 19 Section 19 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION A-NATIONAL SHIPPING AUTHORITY PROCEDURE FOR ACCOMPLISHMENT OF VESSEL REPAIRS UNDER NATIONAL SHIPPING AUTHORITY MASTER LUMP SUM REPAIR CONTRACT-NSA-LUMPSUMREP...

The super elongation complex (SEC) and MLL in development and disease

PubMed Central

Smith, Edwin; Lin, Chengqi; Shilatifard, Ali

2011-01-01

Transcriptional regulation at the level of elongation is vital for the control of gene expression and metazoan development. The mixed lineage leukemia (MLL) protein and its Drosophila homolog, Trithorax, which exist within COMPASS (complex of proteins associated with Set1)-like complexes, are master regulators of development. They are required for proper homeotic gene expression, in part through methylation of histone H3 on Lys 4. In humans, the MLL gene is involved in a large number of chromosomal translocations that create chimeric proteins, fusing the N terminus of MLL to several proteins that share little sequence similarity. Several frequent translocation partners of MLL were found recently to coexist in a super elongation complex (SEC) that includes known transcription elongation factors such as eleven-nineteen lysine-rich leukemia (ELL) and P-TEFb. Importantly, the SEC is required for HOX gene expression in leukemic cells, suggesting that chromosomal translocations involving MLL could lead to the overexpression of HOX and other genes through the involvement of the SEC. Here, we review the normal developmental roles of MLL and the SEC, and how MLL fusion proteins can mediate leukemogenesis. PMID:21460034

Uptake and effectiveness of the Children's Fitness Tax Credit in Canada: the rich get richer.

PubMed

Spence, John C; Holt, Nicholas L; Dutove, Julia K; Carson, Valerie

2010-06-21

The Government of Canada implemented a Children's Fitness Tax Credit (CFTC) in 2007 which allows a non-refundable tax credit of up to $500 to register a child in an eligible physical activity (PA) program. The purposes of this study were to assess whether the awareness, uptake, and perceived effectiveness of this tax credit varied by household income among Canadian parents. An internet-based panel survey was conducted in March 2009 with a representative sample of 2135 Canadians. Of those, parents with children aged 2 to 18 years of age (n = 1004) were asked if their child was involved in organized PA programs (including dance and sports), the associated costs to register their child in these programs, awareness of the CFTC, if they had claimed the CFTC for the tax year 2007, and whether they planned to claim it in the upcoming year. Parents were also asked if they believed the CFTC has lead to their child being more involved in PA programs. Among parents, 54.4% stated their child was in organized PA and 55.5% were aware of the CFTC. Parents in the lowest income quartile were significantly less aware and less likely to claim the CFTC than other income groups. Among parents who had claimed the CFTC, few (15.6%) believed it had increased their child's participation in PA programs. More than half of Canadian parents with children have claimed the CFTC. However, the tax credit appears to benefit the wealthier families in Canada.

Hands on the sun: Teaching SEC science through hands on inquiery and direct observation

NASA Astrophysics Data System (ADS)

Mayo, L.; Cline, T.; Lewis, E.

2003-04-01

Hands on the Sun is a model partnership between the NASA Sun Earth Connection Education Forum (SECEF), Coronado Instruments, Space Science Institute, NOAO/Kitt Peak, Flandrau Planetarium, Astronomical League, and professional astronomers. This joint venture uses experiential learning, provocative talks, and direct observation in both formal and informal education venues to teach participants (K-12 educators, amateur astronomers, and the general public) about the sun, its impact on the Earth, and the importance of understanding the sun-Earth system. The program consists of three days of workshops and activities including tours and observing sessions on Kitt Peak including the National Solar Observatory, planetarium shows, exhibits on space weather, and professional development workshops targeted primarily at Hispanic public school science teachers which are intended to provide hands on activities demonstrating solar and SEC science that can be integrated into the classroom science curriculum. This talk will describe the many facets of this program and discuss our plans for future events.

76 FR 54463 - Notification of a Joint Public Teleconference of the Chartered Science Advisory Board and Board...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-09-01

... Scientific Counselors (BOSC) to discuss a draft report providing advice on Office of Research and Development... discuss a draft report providing advice on Office of Research and Development's (ORD's) new strategic... advisory report. Additional information about SAB and BOSC advice on new ORD strategic research directions...

14 CFR Sec. 1-1 - Applicability of system of accounts and reports.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Applicability of system of accounts and reports. Sec. 1-1 Section 1-1 Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION... AIR CARRIERS General Accounting Provisions Sec. 1-1 Applicability of system of accounts and reports...

14 CFR Sec. 1-1 - Applicability of system of accounts and reports.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 4 2011-01-01 2011-01-01 false Applicability of system of accounts and reports. Sec. 1-1 Section 1-1 Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION... AIR CARRIERS General Accounting Provisions Sec. 1-1 Applicability of system of accounts and reports...

46 CFR Sec. 3 - When the NSA-WORKSMALREP Contract may be used.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 8 2012-10-01 2012-10-01 false When the NSA-WORKSMALREP Contract may be used. Sec. 3 Section 3 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION A-NATIONAL SHIPPING AUTHORITY... REPAIRS-NSA-WORKSMALREP Sec. 3 When the NSA-WORKSMALREP Contract may be used. This contract may be used...

46 CFR Sec. 3 - When the NSA-WORKSMALREP Contract may be used.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 8 2013-10-01 2013-10-01 false When the NSA-WORKSMALREP Contract may be used. Sec. 3 Section 3 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION A-NATIONAL SHIPPING AUTHORITY... REPAIRS-NSA-WORKSMALREP Sec. 3 When the NSA-WORKSMALREP Contract may be used. This contract may be used...

46 CFR Sec. 3 - When the NSA-WORKSMALREP Contract may be used.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 8 2014-10-01 2014-10-01 false When the NSA-WORKSMALREP Contract may be used. Sec. 3 Section 3 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION A-NATIONAL SHIPPING AUTHORITY... REPAIRS-NSA-WORKSMALREP Sec. 3 When the NSA-WORKSMALREP Contract may be used. This contract may be used...

46 CFR Sec. 3 - When the NSA-WORKSMALREP Contract may be used.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 8 2010-10-01 2010-10-01 false When the NSA-WORKSMALREP Contract may be used. Sec. 3 Section 3 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION A-NATIONAL SHIPPING AUTHORITY... REPAIRS-NSA-WORKSMALREP Sec. 3 When the NSA-WORKSMALREP Contract may be used. This contract may be used...

46 CFR Sec. 3 - When the NSA-WORKSMALREP Contract may be used.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 8 2011-10-01 2011-10-01 false When the NSA-WORKSMALREP Contract may be used. Sec. 3 Section 3 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION A-NATIONAL SHIPPING AUTHORITY... REPAIRS-NSA-WORKSMALREP Sec. 3 When the NSA-WORKSMALREP Contract may be used. This contract may be used...

14 CFR Sec. 1-1 - Applicability of system of accounts and reports.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 14 Aeronautics and Space 4 2014-01-01 2014-01-01 false Applicability of system of accounts and reports. Sec. 1-1 Section 1-1 Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION... AIR CARRIERS General Accounting Provisions Sec. 1-1 Applicability of system of accounts and reports...

14 CFR Sec. 1-1 - Applicability of system of accounts and reports.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 14 Aeronautics and Space 4 2013-01-01 2013-01-01 false Applicability of system of accounts and reports. Sec. 1-1 Section 1-1 Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION... AIR CARRIERS General Accounting Provisions Sec. 1-1 Applicability of system of accounts and reports...

22 CFR 214.37 - Public access to committee records.

Code of Federal Regulations, 2010 CFR

2010-04-01

... Section 214.37 Foreign Relations AGENCY FOR INTERNATIONAL DEVELOPMENT ADVISORY COMMITTEE MANAGEMENT Operation of Advisory Committees § 214.37 Public access to committee records. Records maintained in... 212), subject to the general oversight of the A.I.D. Advisory Committee Management Officer. (Sec. 621...

Citizens Advisory Committees.

ERIC Educational Resources Information Center

Stemnock, Suzanne K.

1968-01-01

This document contains the results of a national survey designed to determine the composition and location of permanent citizens advisory committees operating within the nation's school districts. The 52 district-wide, continuing citizens advisory bodies identified by 290 responding school systems are listed alphabetically by State. The following…

The bacterial Sec system is required for the organization and function of the MreB cytoskeleton.

PubMed

Govindarajan, Sutharsan; Amster-Choder, Orna

2017-09-01

The Sec system is responsible for protein insertion, translocation and secretion across membranes in all cells. The bacterial actin homolog MreB controls various processes, including cell wall synthesis, membrane organization and polarity establishment. Here we show that the two systems genetically interact and that components of the Sec system, especially the SecA motor protein, are essential for spatiotemporal organization of MreB in E. coli, as evidenced by the accumulation of MreB at irregular sites in Sec-impaired cells. MreB mislocalization in SecA-defective cells significantly affects MreB-coordinated processes, such as cell wall synthesis, and induce formation of membrane invaginations enriched in high fluidity domains. Additionally, MreB is not recruited to the FtsZ ring in secA mutant cells, contributing to division arrest and cell filamentation. Our results show that all these faults are due to improper targeting of MreB to the membrane in the absence of SecA. Thus, when we reroute RodZ, MreB membrane-anchor, by fusing it to a SecA-independent integral membrane protein and overproducing it, MreB localization is restored and the defect in cell division is corrected. Notably, the RodZ moiety is not properly inserted into the membrane, strongly suggesting that it only serves as a bait for placing MreB around the cell circumference. Finally, we show that MreB localization depends on SecA also in C. crescentus, suggesting that regulation of MreB by the Sec system is conserved in bacteria. Taken together, our data reveal that the secretion system plays an important role in determining the organization and functioning of the cytoskeletal system in bacteria.

Advisory: Definitions, Descriptions, Decisions, Directions.

ERIC Educational Resources Information Center

Galassi, John P.; Gulledge, Suzanne A.; Cox, Nancy D.

Advisory groups have played an essential role in improving the school climate and conditions for young adolescents in schools. How middle school decision makers go about the process of designing or re-designing an advisory program needs to be considered. A discussion is presented of the background information that helps define advisories. It…

Roles of the conserved cytoplasmic region and non-conserved carboxy-terminal region of SecE in Escherichia coli protein translocase.

PubMed

Kontinen, V P; Yamanaka, M; Nishiyama, K; Tokuda, H

1996-06-01

SecE, an essential membrane component of the Escherichia coli protein translocase, consists of 127 amino acid residues. Only a part of the second putative cytoplasmic region comprising some 13 residues is essential for the SecE function as long as the proper topological arrangement is retained. The Trp84 and Pro85 residues of this region are conserved in all eubacterial SecE homologues. The conservation of positively charged residues corresponding to Arg80 and Lys81 is also substantial. We deleted or replaced these residues to assess their roles in the SecE function. Deletion of the Arg80-Lys81 dipeptide did not abolish the SecE function whereas that of Trp84 or Pro85 caused a loss of the function. Strikingly, however, replacement of Pro85 with either Gly, Ser, or Ala, and that of Trp84 with Lys did not abolish the SecE function. These results indicate that the strong conservation of these residues does not reflect their obligatory requirement for the SecE function. A chimeric SecE possessing the cytoplasmic region of the E. coli SecE and the following region of the Bacillus subtilis SecE was able to form the translocation machinery together with SecA, SecY, and SecG. Although a Leu to Arg mutation at position 108 has been thought to cause a loss of signal recognition fidelity and thereby suppress a signal sequence defect, the same mutation at position 111 caused a complete loss of the function. The levels of SecY and SecG in the secEcsE501 mutant, which expresses SecE at a decreased level and is sensitive to low temperature, increased upon the expression of functional SecE derivatives, irrespective of the site of mutation, suggesting that the levels of SecY and SecG are co-operatively determined by the level of functional, but not non-functional, SecE. Based on these results, the SecE function in the translocase is discussed.

75 FR 2880 - Homeland Security Advisory Council

Federal Register 2010, 2011, 2012, 2013, 2014

2010-01-19

... DEPARTMENT OF HOMELAND SECURITY [Docket No. DHS-2009-0160] Homeland Security Advisory Council... advisory committee meeting. SUMMARY: The Homeland Security Advisory Council (HSAC) will meet on February 3.... Mail: Homeland Security Advisory Council, 1100 Hampton Park Boulevard, Mailstop 0850, Capitol Heights...

77 FR 64335 - Notification of a Public Teleconference of the Science Advisory Board; Perchlorate Advisory Panel

Federal Register 2010, 2011, 2012, 2013, 2014

2012-10-19

... Science Advisory Board; Perchlorate Advisory Panel AGENCY: Environmental Protection Agency (EPA). ACTION... announces two public teleconferences of the SAB Perchlorate Advisory Panel to discuss its revised draft... Epidemiological Evidence to Develop a Maximum Contaminant Level Goal (MCLG) for Perchlorate. DATES: The public...

77 FR 25706 - Notice of Advisory Committee Closed Meeting; U.S. Strategic Command Strategic Advisory Group

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-01

..., Command and Control, Science and Technology, Missile Defense. Meeting Accessibility: Pursuant to 5 U.S.C... DEPARTMENT OF DEFENSE Notice of Advisory Committee Closed Meeting; U.S. Strategic Command Strategic Advisory Group AGENCY: Department of Defense. ACTION: Notice of Advisory Committee closed meeting...

16 CFR 1018.12 - Statutory advisory committees.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 16 Commercial Practices 2 2010-01-01 2010-01-01 false Statutory advisory committees. 1018.12 Section 1018.12 Commercial Practices CONSUMER PRODUCT SAFETY COMMISSION GENERAL ADVISORY COMMITTEE MANAGEMENT Establishment of Advisory Committees § 1018.12 Statutory advisory committees. The Commission has...

SEC16A is a RAB10 effector required for insulin-stimulated GLUT4 trafficking in adipocytes

PubMed Central

Bruno, Joanne; Chaudhary, Natasha; Iaea, David

2016-01-01

RAB10 is a regulator of insulin-stimulated translocation of the GLUT4 glucose transporter to the plasma membrane (PM) of adipocytes, which is essential for whole-body glucose homeostasis. We establish SEC16A as a novel RAB10 effector in this process. Colocalization of SEC16A with RAB10 is augmented by insulin stimulation, and SEC16A knockdown attenuates insulin-induced GLUT4 translocation, phenocopying RAB10 knockdown. We show that SEC16A and RAB10 promote insulin-stimulated mobilization of GLUT4 from a perinuclear recycling endosome/TGN compartment. We propose RAB10–SEC16A functions to accelerate formation of the vesicles that ferry GLUT4 to the PM during insulin stimulation. Because GLUT4 continually cycles between the PM and intracellular compartments, the maintenance of elevated cell-surface GLUT4 in the presence of insulin requires accelerated biogenesis of the specialized GLUT4 transport vesicles. The function of SEC16A in GLUT4 trafficking is independent of its previously characterized activity in ER exit site formation and therefore independent of canonical COPII-coated vesicle function. However, our data support a role for SEC23A, but not the other COPII components SEC13, SEC23B, and SEC31, in the insulin stimulation of GLUT4 trafficking, suggesting that vesicles derived from subcomplexes of COPII coat proteins have a role in the specialized trafficking of GLUT4. PMID:27354378

Public Library Advisory Board Handbook.

ERIC Educational Resources Information Center

Bailey, Alvin R., Ed.; And Others

In Texas, public library advisory boards are a recent innovation that have developed as a result of the Council-Manager form of government. This handbook is a response to that development as a compilation of information on the most efficient roles and functions of advisory boards. An advisory board is appointed by the governing body of which the…

The bacterial Sec system is required for the organization and function of the MreB cytoskeleton

PubMed Central

2017-01-01

The Sec system is responsible for protein insertion, translocation and secretion across membranes in all cells. The bacterial actin homolog MreB controls various processes, including cell wall synthesis, membrane organization and polarity establishment. Here we show that the two systems genetically interact and that components of the Sec system, especially the SecA motor protein, are essential for spatiotemporal organization of MreB in E. coli, as evidenced by the accumulation of MreB at irregular sites in Sec-impaired cells. MreB mislocalization in SecA-defective cells significantly affects MreB-coordinated processes, such as cell wall synthesis, and induce formation of membrane invaginations enriched in high fluidity domains. Additionally, MreB is not recruited to the FtsZ ring in secA mutant cells, contributing to division arrest and cell filamentation. Our results show that all these faults are due to improper targeting of MreB to the membrane in the absence of SecA. Thus, when we reroute RodZ, MreB membrane-anchor, by fusing it to a SecA-independent integral membrane protein and overproducing it, MreB localization is restored and the defect in cell division is corrected. Notably, the RodZ moiety is not properly inserted into the membrane, strongly suggesting that it only serves as a bait for placing MreB around the cell circumference. Finally, we show that MreB localization depends on SecA also in C. crescentus, suggesting that regulation of MreB by the Sec system is conserved in bacteria. Taken together, our data reveal that the secretion system plays an important role in determining the organization and functioning of the cytoskeletal system in bacteria. PMID:28945742

Uptake and effectiveness of the Children's Fitness Tax Credit in Canada: the rich get richer

PubMed Central

2010-01-01

Background The Government of Canada implemented a Children's Fitness Tax Credit (CFTC) in 2007 which allows a non-refundable tax credit of up to $500 to register a child in an eligible physical activity (PA) program. The purposes of this study were to assess whether the awareness, uptake, and perceived effectiveness of this tax credit varied by household income among Canadian parents. Methods An internet-based panel survey was conducted in March 2009 with a representative sample of 2135 Canadians. Of those, parents with children aged 2 to 18 years of age (n = 1004) were asked if their child was involved in organized PA programs (including dance and sports), the associated costs to register their child in these programs, awareness of the CFTC, if they had claimed the CFTC for the tax year 2007, and whether they planned to claim it in the upcoming year. Parents were also asked if they believed the CFTC has lead to their child being more involved in PA programs. Results Among parents, 54.4% stated their child was in organized PA and 55.5% were aware of the CFTC. Parents in the lowest income quartile were significantly less aware and less likely to claim the CFTC than other income groups. Among parents who had claimed the CFTC, few (15.6%) believed it had increased their child's participation in PA programs. Conclusions More than half of Canadian parents with children have claimed the CFTC. However, the tax credit appears to benefit the wealthier families in Canada. PMID:20565963

75 FR 33616 - Science Advisory Board Staff Office; Notification of Closed Meetings of the Science Advisory...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-14

... Closed Meetings of the Science Advisory Board's Scientific and Technological Achievement Awards Committee... Agency's (EPA), Science Advisory Board (SAB) Staff Office announces a meeting and teleconference of the....gov . The SAB Mailing address is: U.S. EPA Science Advisory Board (1400F), U.S. Environmental...

75 FR 4069 - Science Advisory Board Staff Office; Notification of a Clean Air Scientific Advisory Committee...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-01-26

... ENVIRONMENTAL PROTECTION AGENCY [FRL-9106-8] Science Advisory Board Staff Office; Notification of.... SUMMARY: The Environmental Protection Agency (EPA or Agency) Science Advisory Board (SAB) Staff Office is... may contact Ms. Kyndall Barry, Designated Federal Officer (DFO), EPA Science Advisory Board (1400F), U...

75 FR 10479 - Science Advisory Board Staff Office; Notification of a Clean Air Scientific Advisory Committee...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-08

... ENVIRONMENTAL PROTECTION AGENCY [FRL-9124-1] Science Advisory Board Staff Office; Notification of... Environmental Protection Agency (EPA) Science Advisory Board (SAB) Staff Office announces a public meeting of... may contact Ms. Kyndall Barry, Designated Federal Officer (DFO), EPA Science Advisory Board (1400F), U...

Information Systems: Opportunities Exist to Strengthen SEC’s Oversight of Capacity and Security

DTIC Science & Technology

2001-07-01

Strengthen SEC’s Oversight of Capacity and Security 5 . FUNDING NUMBERS 6. AUTHOR(S) GAO 7. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) 8. PERFORMING...ANSI Std. Z39-18 298-102 Page i GAO-01-863 Information Systems Letter 1 Results in Brief 2 Background 4 Scope and Methodology 5 SEC Uses a Wide Range...or external organizations to conduct the independent reviews. These internal audits are performed cyclically based on an annual risk analysis. SEC

76 FR 63929 - Joint Meeting of the Drug Safety and Risk Management Advisory Committee and the Dermatologic and...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-14

...- 0002. Information regarding special accommodations due to a disability, visitor parking, and... line/phone line to learn about possible modifications before coming to the meeting. Agenda: The Food... advisory committee meetings and will make every effort to accommodate persons with physical disabilities or...

78 FR 20327 - Joint Meeting of the Advisory Committee for Reproductive Health Drugs and the Drug Safety and...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-04-04

... Management Advisory Committee; Amendment of Notice AGENCY: Food and Drug Administration, HHS. ACTION: Notice... focus on postmarketing reports of oil microembolism in the lungs and potential anaphylactic reactions... in regard to oil microembolism and potential anaphylactic reactions reported for those products. This...

SEC sets guidelines for climate risk

DOE Office of Scientific and Technical Information (OSTI.GOV)

NONE

2010-04-15

In a 3--2 vote in late January 2010, the U.S. Securities & Exchange Commission (SEC), the agency in charge of making sure investors are aware of risks associated with financial investments, approved new 'interpretive guidance' for 'disclosure of climate-related business risks.' The new guidelines call for disclosure of anticipated impact of climate change on assets and financial risks associated with compliance costs for existing and pending climate regulations.

76 FR 44912 - Science Advisory Board Staff Office; Notification of Closed Meetings of the Science Advisory...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-07-27

... Closed Meetings of the Science Advisory Board's Scientific and Technological Achievement Awards Committee... Agency's (EPA), Science Advisory Board (SAB) Staff Office announces a meeting and teleconference of the[email protected] . The SAB Mailing address is: U.S. EPA Science Advisory Board (1400R), U.S. Environmental...

High-loading, 1800 ft/sec tip speed transonic compressor fan stage. 1: Aerodynamic and mechanical design

NASA Technical Reports Server (NTRS)

Morris, A. L.; Halle, J. E.; Kennedy, E. E.

1972-01-01

A single stage fan with a tip speed of 1800 ft/sec (548.6m/sec) and hub/tip ratio of 0.5 was designed to produce a pressure ratio of 2.285:1 with an adiabatic efficiency of 84.0%. The design flow per inlet annulus area is 38.7 lbm/sq ft-sec (188.9KG/sqm-sec). Rotor blades have modified multiple-circular-arc and precompression airfoil sections. The stator vanes have multiple-circular-arc airfoil sections.

46 CFR Sec. 3 - Preparation of invoices.

Code of Federal Regulations, 2010 CFR

2010-10-01

... NSA ORDER NO. 47 Sec. 3 Preparation of invoices. (a) Pursuant to Article 4 of the Service Agreement... under the applicable provisions of NSA Order No. 47. (1) Invoices shall be prepared so as to show... provisions of NSA Order No. 47 due the undersigned General Agent for the month of _____ under Service...

46 CFR Sec. 3 - Preparation of invoices.

Code of Federal Regulations, 2012 CFR

2012-10-01

... NSA ORDER NO. 47 Sec. 3 Preparation of invoices. (a) Pursuant to Article 4 of the Service Agreement... under the applicable provisions of NSA Order No. 47. (1) Invoices shall be prepared so as to show... provisions of NSA Order No. 47 due the undersigned General Agent for the month of _____ under Service...

46 CFR Sec. 3 - Preparation of invoices.

Code of Federal Regulations, 2013 CFR

2013-10-01

... NSA ORDER NO. 47 Sec. 3 Preparation of invoices. (a) Pursuant to Article 4 of the Service Agreement... under the applicable provisions of NSA Order No. 47. (1) Invoices shall be prepared so as to show... provisions of NSA Order No. 47 due the undersigned General Agent for the month of _____ under Service...

46 CFR Sec. 3 - Preparation of invoices.

Code of Federal Regulations, 2011 CFR

2011-10-01

... NSA ORDER NO. 47 Sec. 3 Preparation of invoices. (a) Pursuant to Article 4 of the Service Agreement... under the applicable provisions of NSA Order No. 47. (1) Invoices shall be prepared so as to show... provisions of NSA Order No. 47 due the undersigned General Agent for the month of _____ under Service...

46 CFR Sec. 3 - Preparation of invoices.

Code of Federal Regulations, 2014 CFR

2014-10-01

... NSA ORDER NO. 47 Sec. 3 Preparation of invoices. (a) Pursuant to Article 4 of the Service Agreement... under the applicable provisions of NSA Order No. 47. (1) Invoices shall be prepared so as to show... provisions of NSA Order No. 47 due the undersigned General Agent for the month of _____ under Service...

46 CFR Sec. 6 - Awarding of work.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 8 2011-10-01 2011-10-01 false Awarding of work. Sec. 6 Section 6 Shipping MARITIME... of work. (a) Those portions of all bids reflecting the total aggregate cost of the work involved shall be opened publicly. The work shall be awarded to the contractor submitting the lowest qualified...

46 CFR Sec. 6 - Awarding of work.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 8 2010-10-01 2010-10-01 false Awarding of work. Sec. 6 Section 6 Shipping MARITIME... of work. (a) Those portions of all bids reflecting the total aggregate cost of the work involved shall be opened publicly. The work shall be awarded to the contractor submitting the lowest qualified...

78 FR 28207 - Electricity Advisory Committee

Federal Register 2010, 2011, 2012, 2013, 2014

2013-05-14

... DEPARTMENT OF ENERGY Electricity Advisory Committee AGENCY: Office of Electricity Delivery and... a meeting of the Electricity Advisory Committee (EAC). The Federal Advisory Committee Act (Pub. L..., Arlington, Virginia 22203. FOR FURTHER INFORMATION CONTACT: Matthew Rosenbaum, Office of Electricity...

77 FR 29995 - Electricity Advisory Committee

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2012-05-21

... DEPARTMENT OF ENERGY Electricity Advisory Committee AGENCY: Office of Electricity Delivery and... a meeting of the Electricity Advisory Committee (EAC). The Federal Advisory Committee Act (Pub. L... INFORMATION CONTACT: Matthew Rosenbaum, Office of Electricity Delivery and Energy Reliability, U.S. Department...

77 FR 58534 - Electricity Advisory Committee

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2012-09-21

... DEPARTMENT OF ENERGY Electricity Advisory Committee AGENCY: Office of Electricity Delivery and... a meeting of the Electricity Advisory Committee (EAC). The Federal Advisory Committee Act (Pub. L... FURTHER INFORMATION CONTACT: Matthew Rosenbaum, Office of Electricity Delivery and Energy Reliability, U.S...

76 FR 59667 - Electricity Advisory Committee

Federal Register 2010, 2011, 2012, 2013, 2014

2011-09-27

... DEPARTMENT OF ENERGY Electricity Advisory Committee AGENCY: Office of Electricity Delivery and... a meeting of the Electricity Advisory Committee (EAC). The Federal Advisory Committee Act (Pub. L..., Virginia 22203. FOR FURTHER INFORMATION CONTACT: David Meyer, Office of Electricity Delivery and Energy...

75 FR 61454 - Electricity Advisory Committee

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-05

... DEPARTMENT OF ENERGY Electricity Advisory Committee AGENCY: Department of Energy, Office of Electricity Delivery and Energy Reliability. ACTION: Notice of open meeting. SUMMARY: This notice announces the open meeting of the re-established DOE Electricity Advisory Committee. The Federal Advisory...

76 FR 57023 - Science Advisory Board

Federal Register 2010, 2011, 2012, 2013, 2014

2011-09-15

... DEPARTMENT OF COMMERCE National Oceanic and Atmospheric Administration (NOAA) Science Advisory... forth the schedule and proposed agenda of a forthcoming meeting of the NOAA Science Advisory Board. The... date. SUPPLEMENTARY INFORMATION: The Science Advisory Board (SAB) was established by a Decision...

75 FR 54854 - Science Advisory Board

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-09

... DEPARTMENT OF COMMERCE National Oceanic and Atmospheric Administration Science Advisory Board... the schedule and proposed agenda of a forthcoming meeting of the NOAA Science Advisory Board. The... FURTHER INFORMATION CONTACT: Dr. Cynthia Decker, Executive Director, Science Advisory Board, NOAA, Rm...

76 FR 64088 - World Trade Center Health Program Scientific/Technical Advisory Committee (WTCHP STAC or Advisory...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-17

... DEPARTMENT OF HEALTH AND HUMAN SERVICES Centers for Disease Control and Prevention [Docket Number NIOSH-248] World Trade Center Health Program Scientific/Technical Advisory Committee (WTCHP STAC or Advisory Committee), National Institute for Occupational Safety and Health (NIOSH) In accordance with section 10(a)(2) of the Federal Advisory...

50 CFR 100.12 - Local advisory committees.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 50 Wildlife and Fisheries 6 2010-10-01 2010-10-01 false Local advisory committees. 100.12 Section... Structure § 100.12 Local advisory committees. (a) The Board shall establish such local Federal Advisory... in § 100.11. (b) Local Federal Advisory Committees, if established by the Board, shall operate in...

50 CFR 100.12 - Local advisory committees.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 50 Wildlife and Fisheries 8 2011-10-01 2011-10-01 false Local advisory committees. 100.12 Section... Structure § 100.12 Local advisory committees. (a) The Board shall establish such local Federal Advisory... in § 100.11. (b) Local Federal Advisory Committees, if established by the Board, shall operate in...

Crystal structure of (S)-sec-butyl­ammonium l-tartrate monohydrate

PubMed Central

Publicover, Ernlie A.; Kolwich, Jennifer; Stack, Darcie L.; Doué, Alyssa J.; Ylijoki, Kai E. O.

2017-01-01

The title hydrated mol­ecular salt, C4H12N+·C4H5O6 −·H2O, was prepared by deprotonation of enanti­opure l-tartaric acid with racemic sec-butyl­amine in water. Only one enanti­omer was observed crystallographically, resulting from the combination of (S)-sec-butyl­amine with l-tartaric acid. The sec-butyl­ammonium moiety is disordered over two conformations related by rotation around the CH–CH2 bond; the refined occupancy ratio is 0.68 (1):0.32 (1). In the crystal, mol­ecules are linked through a network of O—H⋯O and N—H⋯O hydrogen-bonding inter­actions, between the ammonium H atoms, the tartrate hy­droxy H atoms, and the inter­stitial water, forming a three-dimensional supra­molecular structure. PMID:28529783

76 FR 78717 - Public Meeting of a Joint Subcommittee of the Motor Carrier Safety Advisory Committee and the...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-12-19

... Committee and the Medical Review Board; Obstructive Sleep Apnea AGENCY: Federal Motor Carrier Safety... Safety Advisory Committee (MCSAC) and Medical Review Board (MRB). SUMMARY: FMCSA announces that MCSAC and... ideas and concepts the Agency should consider for potential regulatory changes to the current physical...

76 FR 37103 - Electricity Advisory Committee

Federal Register 2010, 2011, 2012, 2013, 2014

2011-06-24

... DEPARTMENT OF ENERGY Electricity Advisory Committee AGENCY: Office of Electricity Delivery and... a meeting of the Electricity Advisory Committee (EAC). The Federal Advisory Committee Act (Pub. L...: David Meyer, Office of Electricity Delivery and Energy Reliability, U.S. Department of Energy, Forrestal...

77 FR 476 - Science Advisory Board

Federal Register 2010, 2011, 2012, 2013, 2014

2012-01-05

... DEPARTMENT OF COMMERCE National Oceanic and Atmospheric Administration (NOAA) Science Advisory... forth the schedule and proposed agenda of a forthcoming meeting of the NOAA Science Advisory Board. The... INFORMATION: The Science Advisory Board (SAB) was established by a Decision Memorandum dated September 25...

77 FR 14017 - World Trade Center Health Program Scientific/Technical Advisory Committee (WTCHP STAC or Advisory...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-08

... DEPARTMENT OF HEALTH AND HUMAN SERVICES Centers for Disease Control and Prevention World Trade Center Health Program Scientific/Technical Advisory Committee (WTCHP STAC or Advisory Committee), National Institute for Occupational Safety and Health (NIOSH) In accordance with section 10(a)(2) of the Federal Advisory Committee Act (Pub. L. 92-463),...

77 FR 4820 - World Trade Center Health Program Scientific/Technical Advisory Committee (WTCHP STAC or Advisory...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-01-31

... DEPARTMENT OF HEALTH AND HUMAN SERVICES Centers for Disease Control and Prevention World Trade Center Health Program Scientific/Technical Advisory Committee (WTCHP STAC or Advisory Committee), National Institute for Occupational Safety and Health (NIOSH) In accordance with section 10(a)(2) of the Federal Advisory Committee Act (Pub. L. 92-463),...

76 FR 81947 - World Trade Center Health Program Scientific/Technical Advisory Committee (WTCHP STAC or Advisory...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-12-29

... DEPARTMENT OF HEALTH AND HUMAN SERVICES Centers for Disease Control and Prevention World Trade Center Health Program Scientific/Technical Advisory Committee (WTCHP STAC or Advisory Committee), National Institute for Occupational Safety and Health (NIOSH) In accordance with section 10(a)(2) of the Federal Advisory Committee Act (Pub. L. 92-463)...

75 FR 11883 - Science Advisory Board Staff Office; Notification of a Public Meeting of the Science Advisory...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-12

... ENVIRONMENTAL PROTECTION AGENCY [FRL-9126-2] Science Advisory Board Staff Office; Notification of a Public Meeting of the Science Advisory Board (SAB) AGENCY: Environmental Protection Agency (EPA). ACTION: Notice. SUMMARY: The Environmental Protection Agency (EPA) Science Advisory Board (SAB) Staff...

75 FR 6358 - Federal Advisory Committee; Defense Advisory Board for Employer Support of the Guard and Reserve...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-02-09

... DEPARTMENT OF DEFENSE Office of the Secretary Federal Advisory Committee; Defense Advisory Board...; Charter Revision AGENCY: Department of Defense (DoD). ACTION: Federal advisory committee charter. SUMMARY... Committee Reports to include the Under Secretary of Defense (Personnel and Readiness). FOR FURTHER...

77 FR 52335 - Advisory Board on Radiation and Worker Health (ABRWH or Advisory Board), National Institute for...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-29

... DEPARTMENT OF HEALTH AND HUMAN SERVICES Centers for Disease Control and Prevention Advisory Board on Radiation and Worker Health (ABRWH or Advisory Board), National Institute for Occupational Safety.... Background: The Advisory Board was established under the Energy Employees Occupational Illness Compensation...

77 FR 9254 - Advisory Board on Radiation and Worker Health (ABRWH or Advisory Board), National Institute for...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-16

... DEPARTMENT OF HEALTH AND HUMAN SERVICES Centers for Disease Control and Prevention Advisory Board on Radiation and Worker Health (ABRWH or Advisory Board), National Institute for Occupational Safety.... Background: The Advisory Board was established under the Energy Employees Occupational Illness Compensation...

77 FR 69486 - Advisory Board on Radiation and Worker Health (ABRWH or Advisory Board), National Institute for...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-11-19

... DEPARTMENT OF HEALTH AND HUMAN SERVICES Centers for Disease Control and Prevention Advisory Board on Radiation and Worker Health (ABRWH or Advisory Board), National Institute for Occupational Safety... people. Background: The Advisory Board was established under the Energy Employees Occupational Illness...

76 FR 5814 - Advisory Board on Radiation and Worker Health (ABRWH or Advisory Board), National Institute for...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-02-02

... DEPARTMENT OF HEALTH AND HUMAN SERVICES Centers for Disease Control and Prevention Advisory Board on Radiation and Worker Health (ABRWH or Advisory Board), National Institute for Occupational Safety...: The Advisory Board was established under the Energy Employees Occupational Illness Compensation...

75 FR 66769 - Advisory Board on Radiation and Worker Health (ABRWH or Advisory Board), National Institute for...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-29

... DEPARTMENT OF HEALTH AND HUMAN SERVICES Centers for Disease Control and Prevention Advisory Board on Radiation and Worker Health (ABRWH or Advisory Board), National Institute for Occupational Safety...: The Advisory Board was established under the Energy Employees Occupational Illness Compensation...

13 CFR 130.360 - SBDC advisory boards.

Code of Federal Regulations, 2010 CFR

2010-01-01

... the operation of the SBDC network. (2) The advisory board shall be referred to as a State SBDC... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false SBDC advisory boards. 130.360... CENTERS § 130.360 SBDC advisory boards. (a) State/Regional Advisory Boards. (1) The Lead Center must...

13 CFR 130.360 - SBDC advisory boards.

Code of Federal Regulations, 2013 CFR

2013-01-01

... the operation of the SBDC network. (2) The advisory board shall be referred to as a State SBDC... 13 Business Credit and Assistance 1 2013-01-01 2013-01-01 false SBDC advisory boards. 130.360... CENTERS § 130.360 SBDC advisory boards. (a) State/Regional Advisory Boards. (1) The Lead Center must...

13 CFR 130.360 - SBDC advisory boards.

Code of Federal Regulations, 2014 CFR

2014-01-01

... the operation of the SBDC network. (2) The advisory board shall be referred to as a State SBDC... 13 Business Credit and Assistance 1 2014-01-01 2014-01-01 false SBDC advisory boards. 130.360... CENTERS § 130.360 SBDC advisory boards. (a) State/Regional Advisory Boards. (1) The Lead Center must...

13 CFR 130.360 - SBDC advisory boards.

Code of Federal Regulations, 2012 CFR

2012-01-01

... the operation of the SBDC network. (2) The advisory board shall be referred to as a State SBDC... 13 Business Credit and Assistance 1 2012-01-01 2012-01-01 false SBDC advisory boards. 130.360... CENTERS § 130.360 SBDC advisory boards. (a) State/Regional Advisory Boards. (1) The Lead Center must...

13 CFR 130.360 - SBDC advisory boards.

Code of Federal Regulations, 2011 CFR

2011-01-01

... the operation of the SBDC network. (2) The advisory board shall be referred to as a State SBDC... 13 Business Credit and Assistance 1 2011-01-01 2011-01-01 false SBDC advisory boards. 130.360... CENTERS § 130.360 SBDC advisory boards. (a) State/Regional Advisory Boards. (1) The Lead Center must...

78 FR 55692 - Electricity Advisory Committee

Federal Register 2010, 2011, 2012, 2013, 2014

2013-09-11

... DEPARTMENT OF ENERGY Electricity Advisory Committee AGENCY: Office of Electricity Delivery and... a meeting of the Electricity Advisory Committee (EAC). The Federal Advisory Committee Act (Pub. L... Electricity Delivery and Energy Reliability, U.S. Department of Energy, Forrestal Building, Room 8G-017, 1000...

5 CFR 724.403 - Advisory guidelines.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 5 Administrative Personnel 2 2010-01-01 2010-01-01 false Advisory guidelines. 724.403 Section 724.403 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT (CONTINUED) CIVIL SERVICE REGULATIONS... RETALIATION ACT OF 2002 Best Practices § 724.403 Advisory guidelines. OPM will issue advisory guidelines to...

77 FR 58356 - Science Advisory Board

Federal Register 2010, 2011, 2012, 2013, 2014

2012-09-20

... DEPARTMENT OF COMMERCE National Oceanic and Atmospheric Administration Science Advisory Board... the schedule and proposed agenda of a forthcoming meeting of the NOAA Science Advisory Board. The... Science Advisory Board (SAB) was established by a Decision Memorandum dated September 25, 1997, and is the...

77 FR 43090 - Advisory Board on Radiation and Worker Health (ABRWH or Advisory Board), National Institute for...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-23

... DEPARTMENT OF HEALTH AND HUMAN SERVICES Centers for Disease Control and Prevention Advisory Board on Radiation and Worker Health (ABRWH or Advisory Board), National Institute for Occupational Safety... Advisory Board was established under the Energy Employees Occupational Illness Compensation Program Act of...

75 FR 35496 - Advisory Board on Radiation and Worker Health (ABRWH or Advisory Board), National Institute for...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-22

... DEPARTMENT OF HEALTH AND HUMAN SERVICES Centers for Disease Control and Prevention Advisory Board on Radiation and Worker Health (ABRWH or Advisory Board), National Institute for Occupational Safety... Advisory Board was established under the Energy Employees Occupational Illness Compensation Program Act of...

77 FR 32117 - Advisory Board on Radiation and Worker Health (ABRWH or Advisory Board), National Institute for...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-31

... DEPARTMENT OF HEALTH AND HUMAN SERVICES Centers for Disease Control and Prevention Advisory Board on Radiation and Worker Health (ABRWH or Advisory Board), National Institute for Occupational Safety... Advisory Board was established under the Energy Employees Occupational Illness Compensation Program Act of...

76 FR 47590 - Advisory Board on Radiation and Worker Health (ABRWH or Advisory Board), National Institute for...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-08-05

... DEPARTMENT OF HEALTH AND HUMAN SERVICES Centers for Disease Control and Prevention Advisory Board on Radiation and Worker Health (ABRWH or Advisory Board), National Institute for Occupational Safety... Advisory Board was established under the Energy Employees Occupational Illness Compensation Program Act of...

Septal secretion of protein A in Staphylococcus aureus requires SecA and lipoteichoic acid synthesis

PubMed Central

Yu, Wenqi; Missiakas, Dominique

2018-01-01

Surface proteins of Staphylococcus aureus are secreted across septal membranes for assembly into the bacterial cross-wall. This localized secretion requires the YSIRK/GXXS motif signal peptide, however the mechanisms supporting precursor trafficking are not known. We show here that the signal peptide of staphylococcal protein A (SpA) is cleaved at the YSIRK/GXXS motif. A SpA signal peptide mutant defective for YSIRK/GXXS cleavage is also impaired for septal secretion and co-purifies with SecA, SecDF and LtaS. SecA depletion blocks precursor targeting to septal membranes, whereas deletion of secDF diminishes SpA secretion into the cross-wall. Depletion of LtaS blocks lipoteichoic acid synthesis and abolishes SpA precursor trafficking to septal membranes. We propose a model whereby SecA directs SpA precursors to lipoteichoic acid-rich septal membranes for YSIRK/GXXS motif cleavage and secretion into the cross-wall. PMID:29757141

Sec13 safeguards the integrity of the endoplasmic reticulum and organogenesis of the digestive system in zebrafish.

PubMed

Niu, Xubo; Gao, Chuan; Jan Lo, Li; Luo, Yue; Meng, Chunmei; Hong, Jian; Hong, Wanjin; Peng, Jinrong

2012-07-15

The Sec13-Sec31 heterotetramer serves as the outer coat in the COPII complex, which mediates protein trafficking from the endoplasmic reticulum (ER) to the Golgi apparatus. Although it has been studied in depth in yeast and cultured cells, the role of COPII in organogenesis in a multicellular organism has not. We report here that a zebrafish sec13(sq198) mutant, which exhibits a phenotype of hypoplastic digestive organs, has a mutation in the sec13 gene. The mutant gene encodes a carboxyl-terminus-truncated Sec13 that loses its affinity to Sec31a, which leads to disintegration of the ER structure in various differentiated cells in sec13(sq198), including chondrocytes, intestinal epithelial cells and hepatocytes. Disruption of the ER structure activates an unfolded protein response that eventually causes the cells to undergo cell-cycle arrest and cell apoptosis, which arrest the growth of developing digestive organs in the mutant. Our data provide the first direct genetic evidence that COPII function is essential for the organogenesis of the digestive system. Copyright © 2012 Elsevier Inc. All rights reserved.

75 FR 22560 - Federal Advisory Committee; Naval Research Advisory Committee; Charter Renewal

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-29

... DEPARTMENT OF DEFENSE Office of the Secretary Federal Advisory Committee; Naval Research Advisory... 102-3.50, the Department of Defense gives notice that it is renewing the charter for the Naval... independent advice and recommendations to the Secretary of the Navy, the Chief of Naval Operations and the...

14 CFR Sec. 1-7 - Interpretation of accounts.

Code of Federal Regulations, 2010 CFR

2010-01-01

... PROCEEDINGS) ECONOMIC REGULATIONS UNIFORM SYSTEM OF ACCOUNTS AND REPORTS FOR LARGE CERTIFICATED AIR CARRIERS General Accounting Provisions Sec. 1-7 Interpretation of accounts. To the end that uniform accounting may be maintained, questions involving matters of accounting significance which are not clearly provided...

5 CFR 724.403 - Advisory guidelines.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 5 Administrative Personnel 2 2011-01-01 2011-01-01 false Advisory guidelines. 724.403 Section 724... (CONTINUED) IMPLEMENTATION OF TITLE II OF THE NOTIFICATION AND FEDERAL EMPLOYEE ANTIDISCRIMINATION AND RETALIATION ACT OF 2002 Best Practices § 724.403 Advisory guidelines. OPM will issue advisory guidelines to...

5 CFR 724.403 - Advisory guidelines.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 5 Administrative Personnel 2 2013-01-01 2013-01-01 false Advisory guidelines. 724.403 Section 724... (CONTINUED) IMPLEMENTATION OF TITLE II OF THE NOTIFICATION AND FEDERAL EMPLOYEE ANTIDISCRIMINATION AND RETALIATION ACT OF 2002 Best Practices § 724.403 Advisory guidelines. OPM will issue advisory guidelines to...

5 CFR 724.403 - Advisory guidelines.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 5 Administrative Personnel 2 2014-01-01 2014-01-01 false Advisory guidelines. 724.403 Section 724... (CONTINUED) IMPLEMENTATION OF TITLE II OF THE NOTIFICATION AND FEDERAL EMPLOYEE ANTIDISCRIMINATION AND RETALIATION ACT OF 2002 Best Practices § 724.403 Advisory guidelines. OPM will issue advisory guidelines to...

5 CFR 724.403 - Advisory guidelines.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 5 Administrative Personnel 2 2012-01-01 2012-01-01 false Advisory guidelines. 724.403 Section 724... (CONTINUED) IMPLEMENTATION OF TITLE II OF THE NOTIFICATION AND FEDERAL EMPLOYEE ANTIDISCRIMINATION AND RETALIATION ACT OF 2002 Best Practices § 724.403 Advisory guidelines. OPM will issue advisory guidelines to...

75 FR 2542 - Science Advisory Board Staff Office Notification of a Public Meeting of the Science Advisory...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-01-15

... ENVIRONMENTAL PROTECTION AGENCY [FRL-9104-2] Science Advisory Board Staff Office Notification of a Public Meeting of the Science Advisory Board Committee on Science Integration for Decisionmaking AGENCY... Agency) Science Advisory Board (SAB) Staff Office announces a public meeting of the SAB Committee on...

75 FR 9895 - Science Advisory Board Staff Office; Notification of a Public Meeting of the Science Advisory...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-04

... ENVIRONMENTAL PROTECTION AGENCY [FRL-9122-2] Science Advisory Board Staff Office; Notification of a Public Meeting of the Science Advisory Board; Committee on Science Integration for Decision Making... Agency (EPA or Agency) Science Advisory Board (SAB) Staff Office announces a public meeting of the SAB...

76 FR 53671 - Federal Advisory Committee; Defense Intelligence Agency Advisory Board; Closed Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2011-08-29

... DEPARTMENT OF DEFENSE Office of the Secretary Federal Advisory Committee; Defense Intelligence Agency Advisory Board; Closed Meeting AGENCY: Defense Intelligence Agency (DIA), Department of Defense... amended), and 41 CFR 102-3.150 the Department of Defense announces that Defense Intelligence Agency...

Determining the solution conformational entropy of oligosaccharides by SEC with on-line viscometry detection.

PubMed

Morris, Mallory J; Striegel, André M

2014-06-15

Introduced here is a method for determining the solution conformational entropy of oligosaccharides (-ΔS) that relies on the on-line coupling of size-exclusion chromatography (SEC), an entropically-controlled separation technique, and differential viscometry (VISC). Results from this SEC/VISC method were compared, for the same injections of the same sample dissolutions and under identical solvent/temperature conditions, to results from a benchmark SEC/differential refractometry (SEC/DRI) method which has been applied successfully over the last decade to determining -ΔS of a variety of mono-, di-, and oligosaccharides. The accuracy (as compared to SEC/DRI) and precision of SEC/VISC were found to be excellent, as was the sensitivity of the viscometer in the oligomeric region. The experiments presented here contrast three sets of (1→4)-β-d-oligosaccharides, namely manno-, cello-, and N-acetylchitooligosaccharides of degree of polymerization (DP) 2 through 6. For each series, the dependence of -ΔS on DP was found to be monotonic while, between series, differences at each DP could be ascribed to either the additional degrees of freedom imparted by large, multi-atomic substituent groups, or to the presence or absence of additional intramolecular hydrogen bonds, depending on the axial versus equatorial arrangement of particular hydroxyl groups. An hypothesis is advanced to explain the unexpectedly high sensitivity of viscometric detection for low-molar-mass analytes. The method presented can be extended to the analysis of oligosaccharides other than those studied here. Published by Elsevier Ltd.

75 FR 57743 - Unconventional Resources Technology Advisory Committee

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-22

... DEPARTMENT OF ENERGY Unconventional Resources Technology Advisory Committee AGENCY: Department of... of the Unconventional Resources Technology Advisory Committee. The Federal Advisory Committee Act... INFORMATION: Purpose of the Committee: The purpose of the Unconventional Resources Technology Advisory...

78 FR 68430 - Unconventional Resources Technology Advisory Committee

Federal Register 2010, 2011, 2012, 2013, 2014

2013-11-14

... DEPARTMENT OF ENERGY Unconventional Resources Technology Advisory Committee AGENCY: Office of... meeting of the Unconventional Resources Technology Advisory Committee. The Federal Advisory Committee Act... the Unconventional Resources Technology Advisory Committee is to provide advice on development and...

78 FR 58294 - Unconventional Resources Technology Advisory Committee

Federal Register 2010, 2011, 2012, 2013, 2014

2013-09-23

... DEPARTMENT OF ENERGY Unconventional Resources Technology Advisory Committee AGENCY: Office of... meeting of the Unconventional Resources Technology Advisory Committee. The Federal Advisory Committee Act... the Unconventional Resources Technology Advisory Committee is to provide advice on development and...

77 FR 59182 - Unconventional Resources Technology Advisory Committee

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2012-09-26

... DEPARTMENT OF ENERGY Unconventional Resources Technology Advisory Committee AGENCY: Office of... meeting of the Unconventional Resources Technology Advisory Committee. The Federal Advisory Committee Act... INFORMATION: Purpose of the Committee: The purpose of the Unconventional Resources Technology Advisory...

76 FR 77990 - Unconventional Resources Technology Advisory Committee

Federal Register 2010, 2011, 2012, 2013, 2014

2011-12-15

... DEPARTMENT OF ENERGY Unconventional Resources Technology Advisory Committee AGENCY: Office of... meeting of the Unconventional Resources Technology Advisory Committee. The Federal Advisory Committee Act... INFORMATION: Purpose of the Committee: The purpose of the Unconventional Resources Technology Advisory...

78 FR 17924 - U.S. Strategic Command Strategic Advisory Group; Notice of Federal Advisory Committee Closed Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2013-03-25

... DEPARTMENT OF DEFENSE Office of the Secretary U.S. Strategic Command Strategic Advisory Group... following federal advisory committee: U.S. Strategic Command Strategic Advisory Group. DATES: April 18, 2013..., intelligence, and policy-related issues to the Commander, U.S. Strategic Command, during the development of the...

76 FR 71567 - Advisory Board on Radiation and Worker Health (ABRWH or Advisory Board), National Institute for...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-11-18

... DEPARTMENT OF HEALTH AND HUMAN SERVICES Centers for Disease Control and Prevention Advisory Board on Radiation and Worker Health (ABRWH or Advisory Board), National Institute for Occupational Safety and Health (NIOSH) In accordance with section 10(a)(2) of the Federal Advisory Committee Act (Pub. L...

78 FR 69682 - Advisory Board on Radiation and Worker Health (ABRWH or Advisory Board), National Institute for...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-11-20

... DEPARTMENT OF HEALTH AND HUMAN SERVICES Centers for Disease Control and Prevention (CDC) Advisory Board on Radiation and Worker Health (ABRWH or Advisory Board), National Institute for Occupational Safety and Health (NIOSH) In accordance with section 10(a)(2) of the Federal Advisory Committee Act (Pub...

76 FR 26301 - Advisory Board on Radiation and Worker Health (ABRWH or Advisory Board), National Institute for...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-05-06

... DEPARTMENT OF HEALTH AND HUMAN SERVICES Centers for Disease Control and Prevention Advisory Board on Radiation and Worker Health (ABRWH or Advisory Board), National Institute for Occupational Safety and Health (NIOSH) In accordance with section 10(a)(2) of the Federal Advisory Committee Act (Pub. L...

78 FR 70932 - Unconventional Resources Technology Advisory Committee

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2013-11-27

... DEPARTMENT OF ENERGY Unconventional Resources Technology Advisory Committee AGENCY: Office of... meeting of the Unconventional Resources Technology Advisory Committee. The Federal Advisory Committee Act... of the Unconventional Resources Technology Advisory Committee is to provide advice on development and...

75 FR 57743 - Unconventional Resources Technology Advisory Committee

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2010-09-22

... DEPARTMENT OF ENERGY Unconventional Resources Technology Advisory Committee AGENCY: Department of... of the Unconventional Resources Technology Advisory Committee. The Federal Advisory Committee Act...: Purpose of the Committee: The purpose of the Unconventional Resources Technology Advisory Committee is to...

78 FR 53741 - Unconventional Resources Technology Advisory Committee

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2013-08-30

... DEPARTMENT OF ENERGY Unconventional Resources Technology Advisory Committee AGENCY: Office of... meeting of the Unconventional Resources Technology Advisory Committee. The Federal Advisory Committee Act... of the Unconventional Resources Technology Advisory Committee is to provide advice on development and...

75 FR 36346 - Olympic National Forest; Title II Resource Advisory Committee Meeting Advisory

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2010-06-25

... DEPARTMENT OF AGRICULTURE Olympic National Forest; Title II Resource Advisory Committee Meeting Advisory AGENCY: Olympic National Forest, USDA Forest Service. ACTION: Notice of meeting. SUMMARY: The... this meeting will be to review project proposals and provide recommendations for Title II projects to...

77 FR 2133 - Debt Management Advisory Committee Meeting

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2012-01-13

... DEPARTMENT OF THE TREASURY Debt Management Advisory Committee Meeting Notice is hereby given... management advisory committee: Treasury Borrowing Advisory Committee of the Securities Industry and Financial... financing operations. Historically, this advice has been offered by debt management advisory committees...

78 FR 22034 - Debt Management Advisory Committee Meeting

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2013-04-12

... DEPARTMENT OF THE TREASURY Debt Management Advisory Committee Meeting Notice is hereby given... management advisory committee: Treasury Borrowing Advisory Committee of The Securities Industry and Financial... financing operations. Historically, this advice has been offered by debt management advisory committees...

78 FR 62941 - Debt Management Advisory Committee Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2013-10-22

... DEPARTMENT OF THE TREASURY Debt Management Advisory Committee Meeting Notice is hereby given... management advisory committee: Treasury Borrowing Advisory Committee of The Securities Industry and Financial... financing operations. Historically, this advice has been offered by debt management advisory committees...

77 FR 5246 - Unconventional Resources Technology Advisory Committee

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2012-02-02

... DEPARTMENT OF ENERGY Unconventional Resources Technology Advisory Committee AGENCY: Office of... meeting of the Unconventional Resources Technology Advisory Committee. The Federal Advisory Committee Act... Committee: The purpose of the Unconventional Resources Technology Advisory Committee is to provide advice on...

77 FR 63300 - Unconventional Resources Technology Advisory Committee

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2012-10-16

... DEPARTMENT OF ENERGY Unconventional Resources Technology Advisory Committee AGENCY: Office of... meeting of the Unconventional Resources Technology Advisory Committee. The Federal Advisory Committee Act... Committee: The purpose of the Unconventional Resources Technology Advisory Committee is to provide advice on...

76 FR 64992 - Debt Management Advisory Committee; Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-19

... DEPARTMENT OF THE TREASURY Debt Management Advisory Committee; Meeting Notice is hereby given... management advisory committee: Treasury Borrowing Advisory Committee of the Securities Industry and Financial... operations. Historically, this advice has been offered by debt management advisory committees established by...

An allosteric Sec61 inhibitor traps nascent transmembrane helices at the lateral gate

PubMed Central

MacKinnon, Andrew L; Paavilainen, Ville O; Sharma, Ajay; Hegde, Ramanujan S; Taunton, Jack

2014-01-01

Membrane protein biogenesis requires the coordinated movement of hydrophobic transmembrane domains (TMD) from the cytosolic vestibule of the Sec61 channel into the lipid bilayer. Molecular insight into TMD integration has been hampered by the difficulty of characterizing intermediates during this intrinsically dynamic process. In this study, we show that cotransin, a substrate-selective Sec61 inhibitor, traps nascent TMDs in the cytosolic vestibule, permitting detailed interrogation of an early pre-integration intermediate. Site-specific crosslinking revealed the pre-integrated TMD docked to Sec61 near the cytosolic tip of the lateral gate. Escape from cotransin-arrest depends not only on cotransin concentration, but also on the biophysical properties of the TMD. Genetic selection of cotransin-resistant cancer cells uncovered multiple mutations clustered near the lumenal plug of Sec61α, thus revealing cotransin’s likely site of action. Our results suggest that TMD/lateral gate interactions facilitate TMD transfer into the membrane, a process that is allosterically modulated by cotransin binding to the plug. DOI: http://dx.doi.org/10.7554/eLife.01483.001 PMID:24497544

14 CFR Sec. 1-6 - Accounting entities.

Code of Federal Regulations, 2014 CFR

2014-01-01

...), during either of the prior two years exceeds the greater of $1 million per annum or one percent of the... in section 2-1(d), during either of the prior two years exceeds the greater of $1 million or one... 14 Aeronautics and Space 4 2014-01-01 2014-01-01 false Accounting entities. Sec. 1-6 Section 1-6...

14 CFR Sec. 1-6 - Accounting entities.

Code of Federal Regulations, 2013 CFR

2013-01-01

...), during either of the prior two years exceeds the greater of $1 million per annum or one percent of the... in section 2-1(d), during either of the prior two years exceeds the greater of $1 million or one... 14 Aeronautics and Space 4 2013-01-01 2013-01-01 false Accounting entities. Sec. 1-6 Section 1-6...

28 CFR 0.129 - Professional Responsibility Advisory Office.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 28 Judicial Administration 1 2010-07-01 2010-07-01 false Professional Responsibility Advisory... OF JUSTICE 2-Professional Responsibility Advisory Office § 0.129 Professional Responsibility Advisory Office. (a) The Professional Responsibility Advisory Office is headed by a Director appointed by the...

77 FR 47593 - Shoshone Resource Advisory Committee

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-09

... DEPARTMENT OF AGRICULTURE Forest Service Shoshone Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The Shoshone Resource Advisory Committee (Committee... CONTACT: Olga Troxel, Resource Advisory Committee Coordinator, Shoshone National Forest Supervisor's...

ITS Standards Advisory : Dynamic Message Signs (DMS)

DOT National Transportation Integrated Search

2003-01-01

ITS Standards Advisories provide the transportation community with information and guidance on key activities related to ITS standards, with each Advisory focusing on a single ITS application and its corresponding standards. This advisory focuses on ...

16 CFR 16.13 - Renewal of advisory committees.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 16 Commercial Practices 1 2010-01-01 2010-01-01 false Renewal of advisory committees. 16.13 Section 16.13 Commercial Practices FEDERAL TRADE COMMISSION ORGANIZATION, PROCEDURES AND RULES OF PRACTICE ADVISORY COMMITTEE MANAGEMENT § 16.13 Renewal of advisory committees. (a) Any advisory committee...

16 CFR 16.12 - Termination of advisory committees.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 16 Commercial Practices 1 2010-01-01 2010-01-01 false Termination of advisory committees. 16.12 Section 16.12 Commercial Practices FEDERAL TRADE COMMISSION ORGANIZATION, PROCEDURES AND RULES OF PRACTICE ADVISORY COMMITTEE MANAGEMENT § 16.12 Termination of advisory committees. Any advisory committee shall...

16 CFR 1018.13 - Non-statutory advisory committees.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 16 Commercial Practices 2 2010-01-01 2010-01-01 false Non-statutory advisory committees. 1018.13 Section 1018.13 Commercial Practices CONSUMER PRODUCT SAFETY COMMISSION GENERAL ADVISORY COMMITTEE MANAGEMENT Establishment of Advisory Committees § 1018.13 Non-statutory advisory committees. (a) In proposing...

49 CFR 95.7 - Industry advisory committees: Membership.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 49 Transportation 1 2012-10-01 2012-10-01 false Industry advisory committees: Membership. 95.7 Section 95.7 Transportation Office of the Secretary of Transportation ADVISORY COMMITTEES § 95.7 Industry advisory committees: Membership. Each industry advisory committee must be reasonably representative of the...

49 CFR 95.7 - Industry advisory committees: Membership.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 1 2010-10-01 2010-10-01 false Industry advisory committees: Membership. 95.7 Section 95.7 Transportation Office of the Secretary of Transportation ADVISORY COMMITTEES § 95.7 Industry advisory committees: Membership. Each industry advisory committee must be reasonably representative of the...

49 CFR 95.7 - Industry advisory committees: Membership.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 1 2011-10-01 2011-10-01 false Industry advisory committees: Membership. 95.7 Section 95.7 Transportation Office of the Secretary of Transportation ADVISORY COMMITTEES § 95.7 Industry advisory committees: Membership. Each industry advisory committee must be reasonably representative of the...

75 FR 17701 - High Energy Physics Advisory Panel

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-07

... DEPARTMENT OF ENERGY High Energy Physics Advisory Panel AGENCY: Department of Energy, Office of... Physics Advisory Panel (HEPAP). Federal Advisory Committee Act (Pub. L. 92-463, 86 Stat. 770) requires... Energy Physics Advisory Panel; U.S. Department of Energy; SC-25/ Germantown Building, 1000 Independence...

22 CFR 214.32 - Calling of advisory committee meetings.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Calling of advisory committee meetings. 214.32 Section 214.32 Foreign Relations AGENCY FOR INTERNATIONAL DEVELOPMENT ADVISORY COMMITTEE MANAGEMENT Operation of Advisory Committees § 214.32 Calling of advisory committee meetings. (a) No advisory committee...

76 FR 41196 - Shoshone Resource Advisory Committee

Federal Register 2010, 2011, 2012, 2013, 2014

2011-07-13

... DEPARTMENT OF AGRICULTURE Forest Service Shoshone Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The Shoshone Resource Advisory Committee (Committee... INFORMATION CONTACT: Olga Troxel, Resource Advisory Committee Coordinator, Shoshone National Forest Supervisor...

76 FR 12317 - Shoshone Resource Advisory Committee

Federal Register 2010, 2011, 2012, 2013, 2014

2011-03-07

... DEPARTMENT OF AGRICULTURE Forest Service Shoshone Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The Shoshone Resource Advisory Committee (Committee..., Resource Advisory Committee Coordinator, Shoshone National Forest Supervisor's Office, (307) 578-5164...

75 FR 38086 - Technology Advisory Committee Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2010-07-01

... COMMODITY FUTURES TRADING COMMISSION Technology Advisory Committee Meeting The Commodity Futures Trading Commission's (``Commission'') Technology Advisory Committee will conduct a meeting on Wednesday... Technology Advisory Committee, which will inform the Commission of technological issues and developments...

Limit load solution for electron beam welded joints with single edge weld center crack in tension

NASA Astrophysics Data System (ADS)

Lu, Wei; Shi, Yaowu; Li, Xiaoyan; Lei, Yongping

2012-05-01

Limit loads are widely studied and several limit load solutions are proposed to some typical geometry of weldments. However, there are no limit load solutions exist for the single edge crack weldments in tension (SEC(T)), which is also a typical geometry in fracture analysis. The mis-matching limit load for thick plate with SEC(T) are investigated and the special limit load solutions are proposed based on the available mis-matching limit load solutions and systematic finite element analyses. The real weld configurations are simplified as a strip, and different weld strength mis-matching ratio M, crack depth/width ratio a/ W and weld width 2H are in consideration. As a result, it is found that there exists excellent agreement between the limit load solutions and the FE results for almost all the mis-matching ration M, a/ W and ligament-to-weld width ratio ( W-a)/ H. Moreover, useful recommendations are given for evaluating the limit loads of the EBW structure with SEC(T). For the EBW joints with SEC(T), the mis-matching limit loads can be obtained assuming that the components are wholly made of base metal, when M changing from 1.6 to 0.6. When M decreasing to 0.4, the mis-matching limit loads can be obtained assuming that the components are wholly made of base metal only for large value of ( W-a)/ H. The recommendations may be useful for evaluating the limit loads of the EBW structures with SEC(T). The engineering simplifications are given for assessing the limit loads of electron beam welded structure with SEC(T).

77 FR 18786 - Eastern Washington Cascades Provincial Advisory Committee and the Yakima Provincial Advisory...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-28

... DEPARTMENT OF AGRICULTURE Forest Service Eastern Washington Cascades Provincial Advisory Committee and the Yakima Provincial Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting... Chief's 10-Year Stewardship Challenge, Upper Yakima Basin Water Enhancement Project, Holden Mine...

49 CFR 95.11 - Meetings; industry advisory committees.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 1 2010-10-01 2010-10-01 false Meetings; industry advisory committees. 95.11... Meetings; industry advisory committees. (a) Meetings of an industry advisory committee may be held only at...) No industry advisory committee may receive, compile, or discuss data or reports showing the current...

49 CFR 95.11 - Meetings; industry advisory committees.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 49 Transportation 1 2012-10-01 2012-10-01 false Meetings; industry advisory committees. 95.11... Meetings; industry advisory committees. (a) Meetings of an industry advisory committee may be held only at...) No industry advisory committee may receive, compile, or discuss data or reports showing the current...

49 CFR 95.11 - Meetings; industry advisory committees.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 1 2011-10-01 2011-10-01 false Meetings; industry advisory committees. 95.11... Meetings; industry advisory committees. (a) Meetings of an industry advisory committee may be held only at...) No industry advisory committee may receive, compile, or discuss data or reports showing the current...

77 FR 56607 - Shoshone Resource Advisory Committee

Federal Register 2010, 2011, 2012, 2013, 2014

2012-09-13

... DEPARTMENT OF AGRICULTURE Forest Service Shoshone Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The Shoshone Resource Advisory Committee (Committee) is... call. FOR FURTHER INFORMATION CONTACT: Olga Troxel, Resource Advisory Committee Coordinator, Shoshone...

Analytical Support for the Joint Chiefs of Staff: The WSEG Experience, 1948-1976

DTIC Science & Technology

1979-07-01

high-level officials who participated ŕ’"Directive, Weapons Systems Evaluations Group," En ( Losure to SecDef Memorandum for the Joint Chiefs of Staff...and the field was becoming estab- lished as an identifiable discipline in its own right. 1 2 By and large, the World War II mob ization of science and...3trategic nuclear weapons, e,, en at the expense of other capabilities. They were strongly opposed by defenders of combined operations and balanced force

75 FR 9416 - Advisory Committee Information Hotline

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-02

... Drugs Advisory Committee 3014512542 Peripheral and Central Nervous System Drugs Advisory Committee... Devices Panel 3014512624 Circulatory System Devices Panel 3014512625 Clinical Chemistry and Clinical... about any particular advisory committee meeting, this system will provide interested parties with timely...

22 CFR 214.2 - Definition of advisory committee.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Definition of advisory committee. 214.2 Section 214.2 Foreign Relations AGENCY FOR INTERNATIONAL DEVELOPMENT ADVISORY COMMITTEE MANAGEMENT General § 214.2 Definition of advisory committee. (a) The term advisory committee is defined in section 3(2) of...

10 CFR 7.4 - Establishment of advisory committees.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 10 Energy 1 2012-01-01 2012-01-01 false Establishment of advisory committees. 7.4 Section 7.4 Energy NUCLEAR REGULATORY COMMISSION ADVISORY COMMITTEES § 7.4 Establishment of advisory committees. (a) An NRC advisory committee may be established under this part only if its establishment— (1) Is...

10 CFR 7.4 - Establishment of advisory committees.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 10 Energy 1 2014-01-01 2014-01-01 false Establishment of advisory committees. 7.4 Section 7.4 Energy NUCLEAR REGULATORY COMMISSION ADVISORY COMMITTEES § 7.4 Establishment of advisory committees. (a) An NRC advisory committee may be established under this part only if its establishment— (1) Is...

10 CFR 7.4 - Establishment of advisory committees.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 10 Energy 1 2013-01-01 2013-01-01 false Establishment of advisory committees. 7.4 Section 7.4 Energy NUCLEAR REGULATORY COMMISSION ADVISORY COMMITTEES § 7.4 Establishment of advisory committees. (a) An NRC advisory committee may be established under this part only if its establishment— (1) Is...

10 CFR 7.4 - Establishment of advisory committees.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 10 Energy 1 2010-01-01 2010-01-01 false Establishment of advisory committees. 7.4 Section 7.4 Energy NUCLEAR REGULATORY COMMISSION ADVISORY COMMITTEES § 7.4 Establishment of advisory committees. (a) An NRC advisory committee may be established under this part only if its establishment— (1) Is...

10 CFR 7.4 - Establishment of advisory committees.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 10 Energy 1 2011-01-01 2011-01-01 false Establishment of advisory committees. 7.4 Section 7.4 Energy NUCLEAR REGULATORY COMMISSION ADVISORY COMMITTEES § 7.4 Establishment of advisory committees. (a) An NRC advisory committee may be established under this part only if its establishment— (1) Is...

76 FR 3081 - Shoshone Resource Advisory Committee

Federal Register 2010, 2011, 2012, 2013, 2014

2011-01-19

... DEPARTMENT OF AGRICULTURE Forest Service Shoshone Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The Shoshone Resource Advisory Committee (Committee... meeting will be held via conference call. FOR FURTHER INFORMATION CONTACT: Olga Troxel, Resource Advisory...

78 FR 64910 - Virginia Resource Advisory Committee

Federal Register 2010, 2011, 2012, 2013, 2014

2013-10-30

... DEPARTMENT OF AGRICULTURE Forest Service Virginia Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meetings. SUMMARY: The Virginia Resource Advisory Committee will meet in... Resource Advisory Committee meetings are subject to change or cancellation. Contact Michael Williams...

75 FR 46902 - Ketchikan Resource Advisory Committee

Federal Register 2010, 2011, 2012, 2013, 2014

2010-08-04

... DEPARTMENT OF AGRICULTURE Forest Service Ketchikan Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The Ketchikan Resource Advisory Committee will meet in..., 3031 Tongass Avenue, Ketchikan, Alaska. Send written comments to Ketchikan Resource Advisory Committee...

77 FR 47592 - Ketchikan Resource Advisory Committee

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-09

... DEPARTMENT OF AGRICULTURE Forest Service Ketchikan Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The Ketchikan Resource Advisory Committee will meet in... District, 3031 Tongass Avenue, Ketchikan, Alaska. Send written comments to Ketchikan Resource Advisory...

77 FR 51512 - Allegheny Resource Advisory Committee

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-24

... DEPARTMENT OF AGRICULTURE Forest Service Allegheny Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The Allegheny Resource Advisory Committee will meet in...: The following business will be conducted: Allegheny Resource Advisory Committee members will solicit...

76 FR 23537 - Resource Advisory Committee Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-27

... DEPARTMENT OF AGRICULTURE Resource Advisory Committee Meeting AGENCY: Forest Service, USDA. ACTION: Notice of Public Meeting, Sabine National Forest Resource Advisory Committee. SUMMARY: In accordance with.... Department of Agriculture, Forest Service, Sabine National Forest Resource Advisory Committee (RAC) meeting...

77 FR 18797 - Federal Advisory Committee; Defense Intelligence Agency (DIA) Advisory Board; Closed Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-28

... DEPARTMENT OF DEFENSE Office of the Secretary Federal Advisory Committee; Defense Intelligence... meeting necessarily includes discussions of classified information relating to DIA's intelligence... capabilities in support of current intelligence operations. Agenda May 2, 2012 S 8:30 a.m Convene Advisory...

76 FR 70425 - Federal Advisory Committee; Defense Intelligence Agency (DIA) Advisory Board; Closed Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2011-11-14

... DEPARTMENT OF DEFENSE Office of the Secretary Federal Advisory Committee; Defense Intelligence.... The meetings necessarily include discussions of classified information relating to DIA's intelligence... current intelligence operations. Agenda December 2, 2011 8:30 a.m Convene Advisory Mr. William Caniano...

77 FR 2277 - Federal Advisory Committee; Defense Intelligence Agency (DIA) Advisory Board; Closed Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2012-01-17

... DEPARTMENT OF DEFENSE Office of the Secretary Federal Advisory Committee; Defense Intelligence.... The meetings necessarily include discussions of classified information relating to DIA's intelligence... intelligence operations. Agenda February 24, 2012 8:30 a.m Convene Advisory Mr. William Caniano, Board Meeting...

The Human SepSecS-tRNASec Complex Reveals the Mechanism of Selenocysteine Formation

PubMed Central

Palioura, Sotiria; Sherrer, R. Lynn; Steitz, Thomas A.; Söll, Dieter; Simonović, Miljan

2010-01-01

Selenocysteine is the only genetically encoded amino acid in humans whose biosynthesis occurs on its cognate transfer RNA (tRNA). O-Phosphoseryl-tRNA:selenocysteinyl-tRNA synthase (SepSecS) catalyzes the final step of selenocysteine formation by a poorly understood tRNA-dependent mechanism. The crystal structure of human tRNASec in complex with SepSecS, phosphoserine, and thiophosphate, together with in vivo and in vitro enzyme assays, supports a pyridoxal phosphate–dependent mechanism of Sec-tRNASec formation. Two tRNASec molecules, with a fold distinct from other canonical tRNAs, bind to each SepSecS tetramer through their 13–base pair acceptor-TΨC arm (where Ψ indicates pseudouridine). The tRNA binding is likely to induce a conformational change in the enzyme’s active site that allows a phosphoserine covalently attached to tRNASec, but not free phosphoserine, to be oriented properly for the reaction to occur. PMID:19608919

Beach Advisory and Closing Online Notification (BEACON) system

EPA Pesticide Factsheets

Beach Advisory and Closing Online Notification system (BEACON) is a colletion of state and local data reported to EPA about beach closings and advisories. BEACON is the public-facing query of the Program tracking, Beach Advisories, Water quality standards, and Nutrients database (PRAWN) which tracks beach closing and advisory information.

77 FR 16894 - Financial Research Advisory Committee

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-22

... DEPARTMENT OF THE TREASURY Financial Research Advisory Committee AGENCY: Office of Financial Research, Treasury. ACTION: Notice of establishment of the Financial Research Advisory Committee and... is in the public interest to establish the Financial Research Advisory Committee. A Charter for the...

77 FR 10486 - Electricity Advisory Committee Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-22

... DEPARTMENT OF ENERGY Electricity Advisory Committee Meeting AGENCY: Office of Electricity Delivery... announces a meeting of the Electricity Advisory Committee (EAC). The Federal Advisory Committee Act (Pub. L... 20229. FOR FURTHER INFORMATION CONTACT: Matthew Rosenbaum, Office of Electricity Delivery and Energy...

78 FR 9038 - Electricity Advisory Committee Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2013-02-07

... DEPARTMENT OF ENERGY Electricity Advisory Committee Meeting AGENCY: Office of Electricity Delivery... announces a meeting of the Electricity Advisory Committee (EAC). The Federal Advisory Committee Act (Pub. L..., Arlington, Virginia 22203. FOR FURTHER INFORMATION CONTACT: Matthew Rosenbaum, Office of Electricity...