Sample records for kalda dino scaravelli
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NASA Technical Reports Server (NTRS)
Rosenberg, Gary D. (Editor); Wolberg, Donald L. (Editor); Spencer, Randall S. (Editor)
1994-01-01
This document and video represent the proceedings of the first Dino Fest conference, which was unprecedented in bringing together exhibits of dinosaurs and other fossils and attracting many of the world's leading paleontologists and science educators, students and the public. This first Dino Fest consisted of scores of exhibits that included live and fossil plants, invertebrates and vertebrates. Lasting three weeks, the event concluded with a three-day symposium, providing dinosaur experts from around the country a forum to discuss their research and ideas with the public and other scientists. The document presents the talks of many of the scientists. The video is from an interactive television broadcast relayed by a NASA satellite that enabled children at remote locations to ask questions of a panel of dinosaur experts, literally reaching an audience around the world.
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Archaeopteryx: Bringing the Dino-Bird to Life
DOE Office of Scientific and Technical Information (OSTI.GOV)
Bergmann, Uwe
Some 150 million years ago, a strange creature died in a tropical lagoon that today is located in Bavaria, Germany. In 1861, a single feather of this creature was discovered. Not long afterward, a complete fossil was found with the same bird-like feathers but dinosaur-like anatomical features. Darwin had just published 'On the Origin of Species'; could this be the missing link that Darwin's supporters hoped to find? Recently, two of the now eleven discovered Archaeopteryx fossils, and that first feather, were brought to SLAC, where, using the intense X-ray beam, researchers searched for the chemical remains of the originalmore » living creatures. Please join us for this lecture, which will explain how the studies attempt to bring the original dino-bird back to life.« less
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78 FR 77709 - Notice of Extension of Concession Contracts
Federal Register 2010, 2011, 2012, 2013, 2014
2013-12-24
...-04 3-D River Visions, Inc. Canyonlands National Park. DINO001-04 Adventure Bound, Inc... Dinosaur National Monument. DINO002-04 American River Touring Dinosaur National Association, Inc. Monument. DINO003-04 Outward Bound West..... Dinosaur National Monument. DINO005-04 Holiday River Dinosaur National...
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Retrieval of Missing Spliced Leader in Dinoflagellates
Zhang, Huan; Lin, Senjie
2009-01-01
Spliced leader (SL) trans-splicing has recently been shown to be a common mRNA processing mechanism in dinoflagellates, in which a short (22-nt) sequence, DCCGUAGCCAUUUUGGCUCAAG (D = U, A, or G), is transplanted from the 5′-end of a small non-coding RNA (SL RNA) to the 5′ end of mRNA molecules. The widespread existence of the mechanism in dinoflagellates has been demonstrated by detection of this SL (DinoSL) in a wide phylogenetic range of dinoflagellates. Furthermore, the presence of DinoSL in the transcripts of highly diverse groups of nuclear-encoded genes has led us to postulate that SL trans-splicing is universal in dinoflagellate nuclear genome. However, some observations inconsistent to this postulation have been reported, exemplified by a recent article reporting apparent absence of DinoSL in the transcripts of some nuclear-encoded genes in Amphidinium carterae. Absence of SL in these gene transcripts would have important implication on gene regulation in dinoflagellates and utility of DinoSL as a universal dinoflagellate-specific primer to study dinoflagellate transcriptomics. In this study, we re-examined transcripts of these genes and found that all of them actually contained DinoSL. Therefore, results to date are consistent to our initial postulation that DinoSL occurs in all dinoflagellate nuclear-encoded mRNAs. PMID:19122814
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la Marca, Giancarlo; Giocaliere, Elisa; Malvagia, Sabrina; Villanelli, Fabio; Funghini, Silvia; Ombrone, Daniela; Della Bona, Maria; Forni, Giulia; Canessa, Clementina; Ricci, Silvia; Romano, Francesca; Guerrini, Renzo; Resti, Massimo; Azzari, Chiara
2016-04-01
Purine nucleoside phosphorylase (PNP) deficiency has been recently introduced in the newborn screening program in Tuscany. In order to improve the PNP screening efficiency, we developed a 2nd tier test to quantify PNP primary markers deoxyguanosine (dGuo) and deoxyinosine (dIno). Dried blood spots (DBS) samples were extracted with 200 μL of methanol and 100 μL of water (by two steps). Internal standards were added at a final concentration of 10 μmol/L. After extraction, samples were analysed by LC-MS/MS. The chromatographic run was performed in gradient mode by using a Synergi Fusion column. The assay was linear over a concentration range of 0.05-50 μmol/L (R2>0.999) for dGuo and 0.5-50 μmol/L (R2>0.998) for dIno. Intra- and interassay imprecision (mean CVs) for dIno and dGuo ranged from 2.9% to 12%. Limit of quantitaion (LOQ) were found to be 0.05 μmol/L and 0.5 μmol/L for dGuo and dIno, respectively. The reference ranges, obtained by measuring dGuo and dIno concentrations on DBS, were close to zero for both biomarkers. Moreover, DBS samples from seven patients with confirmed PNP were retrospectively evaluated and correctly identified. The LC-MS/MS method can reliably measure dIno and dGuo in DBS for the diagnosis of PNP. Validation data confirm the present method is characterised by good reproducibility, accuracy and imprecision for the quantitation of dIno and dGuo. The assay also appears suitable for use in monitoring treatment of PNP patients.
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ERIC Educational Resources Information Center
Clary, Renee; Wandersee, James
2011-01-01
This article discusses a creative visualization project to motivate and engage students. Students depict a dinosaur in its ecosystem and include all three elements of the environment: air, land, and water. Students explore scientific content in evolution, natural selection, food webs, ecosystems and geologic time. (Contains 6 figures.)
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Begemann, Petra; Boysen, Gunnar; Georgieva, Nadia I.; Sangaiah, Ramiah; Koshlap, Karl M.; Koc, Hasan; Zhang, Daping; Golding, Bernard T.; Gold, Avram; Swenberg, James A.
2011-01-01
Isoprene, the 2-methyl analog of 1,3-butadiene, is ubiquitous in the environment, with major contributions to total isoprene emissions stemming from natural processes despite the compound being a bulk industrial chemical. Additionally, isoprene is a combustion product and a major component in cigarette smoke. Isoprene has been classified as possibly carcinogenic to humans (group 2B) by IARC and as reasonably anticipated to be a human carcinogen by the National Toxicology Program. Isoprene, like butadiene, requires metabolic activation to reactive epoxides to exhibit its carcinogenic properties. The mode of action has been postulated to be that of a genotoxic carcinogen, with formation of promutagenic DNA adducts being essential for mutagenesis and carcinogenesis. In rodents, isoprene-induced tumors show unique point mutations (A→T transversions) in the K-ras protooncogene at codon 61. Therefore, we investigated adducts formed after reaction of 2′-deoxyadenosine (dAdo1) with the two monoepoxides of isoprene, 2-ethenyl-2-methyloxirane (IP-1,2-O) and propen-2-yloxirane (IP-3,4-O), under physiological conditions. The formation of N1–2′-deoxyinosine (N1-dIno) due to deamination of N1-dAdo adducts was of particular interest, since N1-dIno adducts are suspected to have high mutagenic potential based on in vitro experiments. Major stable adducts were identified by HPLC, UV-Spectrometry and LC-MS/MS and characterized by 1H and 1H,13C HSQC and NMR experiments. Adducts of IP-1,2-O that were fully identified are: R,S-C1-N6-dAdo, R-C2-N6-dAdo, and S-C2-N6-dAdo; adducts of IP-3,4-O are: S-C3-N6-dAdo, R-C3-N6-dAdo, R,S-C4-N6-dAdo, S-C4-N1-dIno, R-C4-N1-dIno, R-C3-N1-dIno, S-C3-N1-dIno, and C3-N7-Ade. Both monoepoxides formed adducts on the external and internal oxirane carbons. This is the first study to describe adducts of isoprene monoepoxides with dAdo. Characterization of adducts formed by isoprene monoepoxides with deoxynucleosides and subsequently with DNA represent
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Linear free energy relationships for selected phthalate esters were used to estimate the rate constants for hydrolysis, biolysis, sediment-water partition coefficients, and biosorption required for modeling. The fate and transport behavior of dimethyl, diethyl, di-n-butyl, di-n-o...
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Cognitive Diagnostic Analysis Using Hierarchically Structured Skills
ERIC Educational Resources Information Center
Su, Yu-Lan
2013-01-01
This dissertation proposes two modified cognitive diagnostic models (CDMs), the deterministic, inputs, noisy, "and" gate with hierarchy (DINA-H) model and the deterministic, inputs, noisy, "or" gate with hierarchy (DINO-H) model. Both models incorporate the hierarchical structures of the cognitive skills in the model estimation…
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Statistical Analysis of Q-matrix Based Diagnostic Classification Models
Chen, Yunxiao; Liu, Jingchen; Xu, Gongjun; Ying, Zhiliang
2014-01-01
Diagnostic classification models have recently gained prominence in educational assessment, psychiatric evaluation, and many other disciplines. Central to the model specification is the so-called Q-matrix that provides a qualitative specification of the item-attribute relationship. In this paper, we develop theories on the identifiability for the Q-matrix under the DINA and the DINO models. We further propose an estimation procedure for the Q-matrix through the regularized maximum likelihood. The applicability of this procedure is not limited to the DINA or the DINO model and it can be applied to essentially all Q-matrix based diagnostic classification models. Simulation studies are conducted to illustrate its performance. Furthermore, two case studies are presented. The first case is a data set on fraction subtraction (educational application) and the second case is a subsample of the National Epidemiological Survey on Alcohol and Related Conditions concerning the social anxiety disorder (psychiatric application). PMID:26294801
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1991-05-07
Prehistoric Yucatan Impact Crater: 'The infamous K-T impact event 65 million years ago (64.98 plus or minus 50,000 years), imagined here just as the bolide strikes, as seen by some high flying Pteranodons. Artwork for NASA by Don Davis. Acrylics and airbrush inks on board.' (nicknamed the Dino Killer)
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Atochina-Vasserman, Elena N.; Massa, Christopher B.; Birkelbach, Bastian; Guo, Chang-Jiang; Scott, Pamela; Haenni, Beat; Beers, Michael F.; Ochs, Matthias; Gow, Andrew J.
2015-01-01
Surfactant protein D (SP-D) modulates the lung's immune system. Its absence leads to NOS2-independent alveolar lipoproteinosis and NOS2-dependent chronic inflammation, which is critical for early emphysematous remodeling. With aging, SP-D knockout mice develop an additional interstitial fibrotic component. We hypothesize that this age-related interstitial septal wall remodeling is mediated by NOS2. Using invasive pulmonary function testing such as the forced oscillation technique and quasistatic pressure-volume perturbation and design-based stereology, we compared 29-wk-old SP-D knockout (Sftpd−/−) mice, SP-D/NOS2 double-knockout (DiNOS) mice, and wild-type mice (WT). Structural changes, including alveolar epithelial surface area, distribution of septal wall thickness, and volumes of septal wall components (alveolar epithelium, interstitial tissue, and endothelium) were quantified. Twenty-nine-week-old Sftpd−/− mice had preserved lung mechanics at the organ level, whereas elastance was increased in DiNOS. Airspace enlargement and loss of surface area of alveolar epithelium coexist with increased septal wall thickness in Sftpd−/− mice. These changes were reduced in DiNOS, and compared with Sftpd−/− mice a decrease in volumes of interstitial tissue and alveolar epithelium was found. To understand the effects of lung pathology on measured lung mechanics, structural data were used to inform a computational model, simulating lung mechanics as a function of airspace derecruitment, septal wall destruction (loss of surface area), and septal wall thickening. In conclusion, NOS2 mediates remodeling of septal walls, resulting in deposition of interstitial tissue in Sftpd−/−. Forward modeling linking structure and lung mechanics describes the complex mechanical properties by parenchymatous destruction (emphysema), interstitial remodeling (septal wall thickening), and altered recruitability of acinar airspaces. PMID:26320150
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la Marca, Giancarlo; Canessa, Clementina; Giocaliere, Elisa; Romano, Francesca; Malvagia, Sabrina; Funghini, Silvia; Moriondo, Maria; Valleriani, Claudia; Lippi, Francesca; Ombrone, Daniela; Della Bona, Maria Luisa; Speckmann, Carsten; Borte, Stephan; Brodszki, Nicholas; Gennery, Andrew R; Weinacht, Katja; Celmeli, Fatih; Pagel, Julia; de Martino, Maurizio; Guerrini, Renzo; Wittkowski, Helmut; Santisteban, Ines; Bali, Pawan; Ikinciogullari, Aydan; Hershfield, Michael; Notarangelo, Luigi D; Resti, Massimo; Azzari, Chiara
2014-07-01
Purine nucleoside phosphorylase (PNP) deficiency is a rare form of autosomal recessive combined primary immunodeficiency caused by a enzyme defect leading to the accumulation of inosine, 2'-deoxy-inosine (dIno), guanosine, and 2'-deoxy-guanosine (dGuo) in all cells, especially lymphocytes. Treatments are available and curative for PNP deficiency, but their efficacy depends on the early approach. PNP-combined immunodeficiency complies with the criteria for inclusion in a newborn screening program. This study evaluate whether mass spectrometry can identify metabolite abnormalities in dried blood spots (DBSs) from affected patients, with the final goal of individuating the disease at birth during routine newborn screening. DBS samples from 9 patients with genetically confirmed PNP-combined immunodeficiency, 10,000 DBS samples from healthy newborns, and 240 DBSs from healthy donors of different age ranges were examined. Inosine, dIno, guanosine, and dGuo were tested by using tandem mass spectrometry (TMS). T-cell receptor excision circle (TREC) and kappa-deleting recombination excision circle (KREC) levels were evaluated by using quantitative RT-PCR only for the 2 patients (patients 8 and 9) whose neonatal DBSs were available. Mean levels of guanosine, inosine, dGuo, and dIno were 4.4, 133.3, 3.6, and 3.8 μmol/L, respectively, in affected patients. No indeterminate or false-positive results were found. In patient 8 TREC levels were borderline and KREC levels were abnormal; in patient 9 TRECs were undetectable, whereas KREC levels were normal. TMS is a valid method for diagnosis of PNP deficiency on DBSs of affected patients at a negligible cost. TMS identifies newborns with PNP deficiency, whereas TREC or KREC measurement alone can fail. Copyright © 2014 American Academy of Allergy, Asthma & Immunology. Published by Mosby, Inc. All rights reserved.
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Measurement of psychological disorders using cognitive diagnosis models.
Templin, Jonathan L; Henson, Robert A
2006-09-01
Cognitive diagnosis models are constrained (multiple classification) latent class models that characterize the relationship of questionnaire responses to a set of dichotomous latent variables. Having emanated from educational measurement, several aspects of such models seem well suited to use in psychological assessment and diagnosis. This article presents the development of a new cognitive diagnosis model for use in psychological assessment--the DINO (deterministic input; noisy "or" gate) model--which, as an illustrative example, is applied to evaluate and diagnose pathological gamblers. As part of this example, a demonstration of the estimates obtained by cognitive diagnosis models is provided. Such estimates include the probability an individual meets each of a set of dichotomous Diagnostic and Statistical Manual of Mental Disorders (text revision [DSM-IV-TR]; American Psychiatric Association, 2000) criteria, resulting in an estimate of the probability an individual meets the DSM-IV-TR definition for being a pathological gambler. Furthermore, a demonstration of how the hypothesized underlying factors contributing to pathological gambling can be measured with the DINO model is presented, through use of a covariance structure model for the tetrachoric correlation matrix of the dichotomous latent variables representing DSM-IV-TR criteria. Copyright 2006 APA
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Application of summative content analysis to a postal questionnaire.
Griffiths, Thomas Howard
2016-01-01
Content analysis of replies to closed questions in questionnaires can be undertaken to understand remarks that may explain the responses, provide illustrative examples of issues raised in the questionnaire, define new issues or issues of importance that were not covered in the questionnaire and inform the design of new questions in future surveys. To discuss the usefulness of summative content analysis to free text in postal questionnaires. Content analysis provides useful comparative insights between two respondent groups in the case example provided. Five themes emerged: poor understanding of the concept of 'patient lateral transfer work technique' and the direct instrument nursing observation (DINO) instrument's key directions; outcomes of patient transfer; positive responses; manual handling risk; and poor translation into English of DINO. Respondents need an opportunity to clarify their responses to questionnaires using free text, to provide insight into their understanding of the question being asked, understanding of the concept or construct being discussed, and data triangulation through the confirmation of item responses and free-text comments. Responses to questions in a postal questionnaire and the opportunity for free-text commentary by respondents enable the identification of hidden meanings behind tickbox responses to questions.
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Of dups and dinos: evolution at the K/Pg boundary.
Lohaus, Rolf; Van de Peer, Yves
2016-04-01
Fifteen years into sequencing entire plant genomes, more than 30 paleopolyploidy events could be mapped on the tree of flowering plants (and many more when also transcriptome data sets are considered). While some genome duplications are very old and have occurred early in the evolution of dicots and monocots, or even before, others are more recent and seem to have occurred independently in many different plant lineages. Strikingly, a majority of these duplications date somewhere between 55 and 75 million years ago (mya), and thus likely correlate with the K/Pg boundary. If true, this would suggest that plants that had their genome duplicated at that time, had an increased chance to survive the most recent mass extinction event, at 66mya, which wiped out a majority of plant and animal life, including all non-avian dinosaurs. Here, we review several processes, both neutral and adaptive, that might explain the establishment of polyploid plants, following the K/Pg mass extinction. Copyright © 2016 The Authors. Published by Elsevier Ltd.. All rights reserved.
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2009-05-14
Dan Andrews, Project Manager LCROSS and Craig Tooley, Project Manager LRO (Lunar Reconnaissance Orbiter) in front of Spacecraft at the Astrotech facility titusville, FL during LRO/LCROSS media photo op event. The LCROSS mission's objective is to confirm the presence or absence of water ice in a permanently shadowed crater at the moon's South Pole. (Photo by Jonis Dino AMES PAO, premission for use granted by Dan Andrews, image owner)
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Chiu, Chia-Yi; Köhn, Hans-Friedrich
2016-09-01
The asymptotic classification theory of cognitive diagnosis (ACTCD) provided the theoretical foundation for using clustering methods that do not rely on a parametric statistical model for assigning examinees to proficiency classes. Like general diagnostic classification models, clustering methods can be useful in situations where the true diagnostic classification model (DCM) underlying the data is unknown and possibly misspecified, or the items of a test conform to a mix of multiple DCMs. Clustering methods can also be an option when fitting advanced and complex DCMs encounters computational difficulties. These can range from the use of excessive CPU times to plain computational infeasibility. However, the propositions of the ACTCD have only been proven for the Deterministic Input Noisy Output "AND" gate (DINA) model and the Deterministic Input Noisy Output "OR" gate (DINO) model. For other DCMs, there does not exist a theoretical justification to use clustering for assigning examinees to proficiency classes. But if clustering is to be used legitimately, then the ACTCD must cover a larger number of DCMs than just the DINA model and the DINO model. Thus, the purpose of this article is to prove the theoretical propositions of the ACTCD for two other important DCMs, the Reduced Reparameterized Unified Model and the General Diagnostic Model.
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On the Complexity of Item Response Theory Models.
Bonifay, Wes; Cai, Li
2017-01-01
Complexity in item response theory (IRT) has traditionally been quantified by simply counting the number of freely estimated parameters in the model. However, complexity is also contingent upon the functional form of the model. We examined four popular IRT models-exploratory factor analytic, bifactor, DINA, and DINO-with different functional forms but the same number of free parameters. In comparison, a simpler (unidimensional 3PL) model was specified such that it had 1 more parameter than the previous models. All models were then evaluated according to the minimum description length principle. Specifically, each model was fit to 1,000 data sets that were randomly and uniformly sampled from the complete data space and then assessed using global and item-level fit and diagnostic measures. The findings revealed that the factor analytic and bifactor models possess a strong tendency to fit any possible data. The unidimensional 3PL model displayed minimal fitting propensity, despite the fact that it included an additional free parameter. The DINA and DINO models did not demonstrate a proclivity to fit any possible data, but they did fit well to distinct data patterns. Applied researchers and psychometricians should therefore consider functional form-and not goodness-of-fit alone-when selecting an IRT model.
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Center for Autonomous Solar Power
2013-06-26
Cell from Sputtering Method, Tara P. Dhakal, Lakshmi K. Ganta, Dino Ferizovic, Surya Rajendran, Daniel Vanhart, Michael A. Seymour and Charles R...Annealing, Tara P. Dhakal, Lakshmi K. Ganta, Charles R. Westgate, 38th IEEE PVSC 2011 conference proceedings, submitted on June 2. 2012 and accepted...on June 14, 2012 Synthesis o/FeS2 Nano Crystals for Ink-Based Solar Cells, Lakshmi Kanth Ganta, Tara P. Dhakal, Surya Rajendran and Charles R
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Battle of Britain Toolbook (User’s Guide)
1995-05-01
look back to the Battle of Britain to determine how lessons therefrom have future application. The British author, Group Captain A.G.B. Vallance ...The Command of the Air. Translated by Dino Ferrari. Washington D.C.: Office of Air Force History, 1983. Estes, Richard H. "Guilio Douhet: More on...Decision" Armour. LXXXUI-3:19-22 (May-June 1974). Kaplan, Philip and Richard Collier. Their Finest Hour / The Battle of Britain Remembered. New York
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The DTIC Review. Volume 5, Number 2. Real-Time Communications
2000-12-01
appreciate your comments. Kurt N. Molholm Administrator THIS PAGE INTENTIONALLY LEFT BLANK The DTIC Review Defense Technical Information Center TABLE OF...assisted by Captain Dino Perone, CW2 Kurt Prokarym, CW2 Earl Johnson, SSG Case, MSG Nancy MacDonald, SGM Jessie Husband, and many other junior non...Navy IT-21 Audit Policy 07 Jul 2000 28 PAGES 01 Sep 1999 61 PAGES PERSONAL AUTHORS: Gevelber, Michael; PERSONAL AUTHORS: Kremer , H. S. Wroblewski
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Setup: What the Air Force Did in Vietnam and Why
1991-06-01
4, no. 2 (Fall 1950) : 12 . 18 . Ibid . 19 . Giulio Douhet, The Command ofthe Air, trans . Dino Ferrari, reprinted by the Office of Air Force History...demilitarized zone (DMZ). Ironically, in November 1954, Gen Tran Van Don and other leading Vietnamese generals suggested that the MAAG organize their...Office ofAir Force History, 1981), 36. 37 . Ibid., 49-50. 38 . According to formerSouth Vietnamese minister ofnational defense Gen Tran Van Don, he and
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Boundary Influences on HAB Phytoplankton Ecology in a Stratification-Enhanced Upwelling Shadow
2014-05-13
localization, tracking and sampling during djal AUV operations (Figs. 12-15). coincident with the cool side of a front (Fig. 12). Abundant gelatinous ...Busse, LB., Mazzillo, F., Silver , M.W.. 2008. The emergence of Cochtodinium along the California Coast (USA). Harmful Algae 7, 337-346. Das, J...p. 89-92. Mazzillo F.F.M.. Ryan J.P.. Silver M.W., 2011 Parasitism as a biological control agent of dino flagellate blooms in the California
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A New Method for in Situ Measurement of Bt-Maize Pollen Deposition on Host-Plant Leaves.
Hofmann, Frieder; Otto, Mathias; Kuhn, Ulrike; Ober, Steffi; Schlechtriemen, Ulrich; Vögel, Rudolph
2011-02-21
Maize is wind pollinated and produces huge amounts of pollen. In consequence, the Cry toxins expressed in the pollen of Bt maize will be dispersed by wind in the surrounding vegetation leading to exposure of non-target organisms (NTO). NTO like lepidopteran larvae may be affected by the uptake of Bt-pollen deposited on their host plants. Although some information is available to estimate pollen deposition on host plants, recorded data are based on indirect measurements such as shaking or washing off pollen, or removing pollen with adhesive tapes. These methods often lack precision and they do not include the necessary information such as the spatial and temporal variation of pollen deposition on the leaves. Here, we present a new method for recording in situ the amount and the distribution of Bt-maize pollen deposited on host plant leaves. The method is based on the use of a mobile digital microscope (Dino-Lite Pro, including DinoCapture software), which can be used in combination with a notebook in the field. The method was evaluated during experiments in 2008 to 2010. Maize pollen could be correctly identified and pollen deposition as well as the spatial heterogeneity of maize pollen deposition was recorded on maize and different lepidopteran host plants (Centaurea scabiosa, Chenopodium album, Rumex spp., Succina pratensis and Urtica dioica) growing adjacent to maize fields.
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A New Method for in Situ Measurement of Bt-Maize Pollen Deposition on Host-Plant Leaves
Hofmann, Frieder; Otto, Mathias; Kuhn, Ulrike; Ober, Steffi; Schlechtriemen, Ulrich; Vögel, Rudolph
2011-01-01
Maize is wind pollinated and produces huge amounts of pollen. In consequence, the Cry toxins expressed in the pollen of Bt maize will be dispersed by wind in the surrounding vegetation leading to exposure of non-target organisms (NTO). NTO like lepidopteran larvae may be affected by the uptake of Bt-pollen deposited on their host plants. Although some information is available to estimate pollen deposition on host plants, recorded data are based on indirect measurements such as shaking or washing off pollen, or removing pollen with adhesive tapes. These methods often lack precision and they do not include the necessary information such as the spatial and temporal variation of pollen deposition on the leaves. Here, we present a new method for recording in situ the amount and the distribution of Bt-maize pollen deposited on host plant leaves. The method is based on the use of a mobile digital microscope (Dino-Lite Pro, including DinoCapture software), which can be used in combination with a notebook in the field. The method was evaluated during experiments in 2008 to 2010. Maize pollen could be correctly identified and pollen deposition as well as the spatial heterogeneity of maize pollen deposition was recorded on maize and different lepidopteran host plants (Centaurea scabiosa, Chenopodium album, Rumex spp., Succina pratensis and Urtica dioica) growing adjacent to maize fields. PMID:26467496
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Deployment and Intelligent Nanosatellite Operations Colorado Final Technical Report
2006-09-28
environmental factors will cause disturbance torques during orbit around the Earth . These factors are solar radiation pressure from the sun , aerodynamic...software. The 3- axis sensing of the magnetometer allows a vector the B- field of the Earth to be sensed. Geopack 2003 then can be utilized with the orbit ...gradient torque can be represented as the following: g, ; 3wo21 Eq. 2-11 where ow is the angular velocity of the spacecraft as it orbits the earth . DINO’s
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Molecular phylogeny of noctilucoid dinoflagellates (Noctilucales, Dinophyceae).
Gómez, Fernando; Moreira, David; López-García, Purificación
2010-07-01
The order Noctilucales or class Noctiluciphyceae encompasses three families of aberrant dinoflagellates (Noctilucaceae, Leptodiscaceae and Kofoidiniaceae) that, at least in some life stages, lack typical dinoflagellate characters such as the ribbon-like transversal flagellum or condensed chromosomes. Noctiluca scintillans, the first dinoflagellate to be described, has been intensively investigated. However, its phylogenetic position based on the small subunit ribosomal DNA (SSU rDNA) sequence is unstable and controversial. Noctiluca has been placed either as an early diverging lineage that diverged after Oxyrrhis and before the dinokaryotes -core dinoflagellates- or as a recent lineage branching from unarmoured dino fl agellates in the order Gymnodiniales. So far, the lack of other noctilucoid sequences has hampered the elucidation of their phylogenetic relationships to other dino fl agellates. Furthermore, even the monophyly of the noctilucoids remained uncertain. We have determined SSU rRNA gene sequences for Kofoidiniaceae, those of the type Spatulodinium (=Gymnodinium) pseudonoctiluca and another Spatulodinium species, as well as of two species of Kofoidinium, and the first gene sequence of Leptodiscaceae, that of Abedinium (=Leptophyllus) dasypus. These taxa were collected from their type localities, the English Channel and the NW Mediterranean Sea, respectively. Phylogenetic analyses place the Noctilucales as a monophyletic group at a basal position close to parasites of the Marine Alveolate Group I (MAGI) and the Syndiniales (MAGII), before the core of dinokaryotic dinoflagellates, although with moderate support. 2010 Elsevier GmbH. All rights reserved.
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Lin, Senjie; Zhang, Huan; Zhuang, Yunyun; Tran, Bao; Gill, John
2010-01-01
Environmental transcriptomics (metatranscriptomics) for a specific lineage of eukaryotic microbes (e.g., Dinoflagellata) would be instrumental for unraveling the genetic mechanisms by which these microbes respond to the natural environment, but it has not been exploited because of technical difficulties. Using the recently discovered dinoflagellate mRNA-specific spliced leader as a selective primer, we constructed cDNA libraries (e-cDNAs) from one marine and two freshwater plankton assemblages. Small-scale sequencing of the e-cDNAs revealed functionally diverse transcriptomes proven to be of dinoflagellate origin. A set of dinoflagellate common genes and transcripts of dominant dinoflagellate species were identified. Further analyses of the dataset prompted us to delve into the existing, largely unannotated dinoflagellate EST datasets (DinoEST). Consequently, all four nucleosome core histones, two histone modification proteins, and a nucleosome assembly protein were detected, clearly indicating the presence of nucleosome-like machinery long thought not to exist in dinoflagellates. The isolation of rhodopsin from taxonomically and ecotypically diverse dinoflagellates and its structural similarity and phylogenetic affinity to xanthorhodopsin suggest a common genetic potential in dinoflagellates to use solar energy nonphotosynthetically. Furthermore, we found 55 cytoplasmic ribosomal proteins (RPs) from the e-cDNAs and 24 more from DinoEST, showing that the dinoflagellate phylum possesses all 79 eukaryotic RPs. Our results suggest that a sophisticated eukaryotic molecular machine operates in dinoflagellates that likely encodes many more unsuspected physiological capabilities and, meanwhile, demonstrate that unique spliced leaders are useful for profiling lineage-specific microbial transcriptomes in situ. PMID:21041634
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Lin, Senjie; Zhang, Huan; Zhuang, Yunyun; Tran, Bao; Gill, John
2010-11-16
Environmental transcriptomics (metatranscriptomics) for a specific lineage of eukaryotic microbes (e.g., Dinoflagellata) would be instrumental for unraveling the genetic mechanisms by which these microbes respond to the natural environment, but it has not been exploited because of technical difficulties. Using the recently discovered dinoflagellate mRNA-specific spliced leader as a selective primer, we constructed cDNA libraries (e-cDNAs) from one marine and two freshwater plankton assemblages. Small-scale sequencing of the e-cDNAs revealed functionally diverse transcriptomes proven to be of dinoflagellate origin. A set of dinoflagellate common genes and transcripts of dominant dinoflagellate species were identified. Further analyses of the dataset prompted us to delve into the existing, largely unannotated dinoflagellate EST datasets (DinoEST). Consequently, all four nucleosome core histones, two histone modification proteins, and a nucleosome assembly protein were detected, clearly indicating the presence of nucleosome-like machinery long thought not to exist in dinoflagellates. The isolation of rhodopsin from taxonomically and ecotypically diverse dinoflagellates and its structural similarity and phylogenetic affinity to xanthorhodopsin suggest a common genetic potential in dinoflagellates to use solar energy nonphotosynthetically. Furthermore, we found 55 cytoplasmic ribosomal proteins (RPs) from the e-cDNAs and 24 more from DinoEST, showing that the dinoflagellate phylum possesses all 79 eukaryotic RPs. Our results suggest that a sophisticated eukaryotic molecular machine operates in dinoflagellates that likely encodes many more unsuspected physiological capabilities and, meanwhile, demonstrate that unique spliced leaders are useful for profiling lineage-specific microbial transcriptomes in situ.
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Hasegawa, Kosuke; Suetsugu, Atsushi; Nakamura, Miki; Matsumoto, Takuro; Kunisada, Takahiro; Shimizu, Masahito; Saji, Shigetoyo; Moriwaki, Hisataka; Bouvet, Michael; Hoffman, Robert M
2016-09-01
Fluorescence-guided surgery (FGS) of cancer is an emerging technology. We have previously shown the importance of resecting both the tumor and the tumor microenvironment (TME) for curative FGS. We also previously developed a syngeneic model using the mouse lymphoma cell line EL-4, expressing red fluorescent protein (EL-4-RFP), growing in green fluorescent protein (GFP) transgenic mice, which we have used in the present report to develop FGS of the tumor microenvironment. EL-4-RFP lymphoma cells were injected subcutaneously in C57/BL6 GFP transgenic mice. EL-4-RFP cells subsequently formed tumors by 35 days after cell transplantation. Using the portable hand-held Dino-Lite digital imaging system, subcutaneous tumors were resected by FGS. Resected tumor tissues were visualized with the Olympus FV1000 confocal microscope. Using the Dino-Lite, subcutaneous tumors and the tumor microenvironment were clearly visualized and resected. In the resected tumor, host stromal cells, including adipocyte-like cells and blood vessels with lymphocytes, were observed by confocal microscopy in addition to cancer cells by color-coded confocal imaging. The cancer cells and stromal cells in the TME were deeply intermingled in a highly-complex pattern. Color-coded FGS is an effective method to completely resect cancer cells along with the stromal cells in the TME which interact in a highly-complex pattern. Microscopically, cancer cells invade the TME and vice versa. To prevent tumor recurrence, it is necessary to resect the TME along with the tumor. Copyright© 2016 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.
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Life Cycle Cost Estimates of the AN/WLR-1G, AN/SLQ-32(V)2, and Conceptual ESM System.
1979-09-01
7 Ah-hofl GS NAVAL OCR" N SYSTEMS CEIIIE IA Dino CA PiM ITA LIFE CYCLE COST C-STIMATES OF TA 4UWLk-1Gu ADULI-2aV)1. AD --4MCtw W S 79 L VM#J 1ALUA...repair OPEVAL Operational evaluation TECHEVAL Technical evaluation 3 It . PRFORING ORNATIOCNMENTATON PAGE BEROGRM COLE IN FO RMT T IIJILS" GVT ACESSON N ...distriuation unRedhCr S. PERFTRINTORNISTATEN NAM A. D baRact to.~odi PROwRA beeFT Rou AS 1S. SUPPRLEMENAR OTESNM N DRS -L. KOYIWORING A(CY NAME 6_VWO d
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Zhang, Huan; Campbell, David A.; Sturm, Nancy R.; Dungan, Christopher F.; Lin, Senjie
2011-01-01
The genus Perkinsus occupies a precarious phylogenetic position. To gain a better understanding of the relationship between perkinsids, dinoflagellates and other alveolates, we analyzed the nuclear-encoded spliced-leader (SL) RNA and mitochondrial genes, intron prevalence, and multi-protein phylogenies. In contrast to the canonical 22-nt SL found in dinoflagellates (DinoSL), P. marinus has a shorter (21-nt) and a longer (22-nt) SL with slightly different sequences than DinoSL. The major SL RNA transcripts range in size between 80–83 nt in P. marinus, and ∼83 nt in P. chesapeaki, significantly larger than the typical ≤56-nt dinoflagellate SL RNA. In most of the phylogenetic trees based on 41 predicted protein sequences, P. marinus branched at the base of the dinoflagellate clade that included the ancient taxa Oxyrrhis and Amoebophrya, sister to the clade of apicomplexans, and in some cases clustered with apicomplexans as a sister to the dinoflagellate clade. Of 104 Perkinsus spp. genes examined 69.2% had introns, a higher intron prevalence than in dinoflagellates. Examination of Perkinsus spp. mitochondrial cytochrome B and cytochrome C oxidase subunit I genes and their cDNAs revealed no mRNA editing, but these transcripts can only be translated when frameshifts are introduced at every AGG and CCC codon as if AGGY codes for glycine and CCCCU for proline. These results, along with the presence of the numerous uncharacterized ‘marine alveolate group I' and Perkinsus-like lineages separating perkinsids from core dinoflagellates, expand support for the affiliation of the genus Perkinsus with an independent lineage (Perkinsozoa) positioned between the phyla of Apicomplexa and Dinoflagellata. PMID:21629701
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NASA Astrophysics Data System (ADS)
Kochańska, Agnieszka
2016-09-01
The romantic landscape park was established in 1824 by order of one of the best-known aristocrats at the time, Princess Dorothea Talleyrand Perigold, Dutchess of Dino. The park is an example of amenity horticulture of artistic value that has survived to this day. The creator of the park was the talented landscape architect Peter Joseph Lenné. In its current state, the park differs significantly from the image on lithographs of the past. Upon carrying out field investigations and comparative studies, it was established that the maintained stand of tress holds high compositional value. The conclusions of the article define the current threats to the longevity of the park as well as highlighting the plan of revalorization works.
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ERIC Educational Resources Information Center
Clary, Renee; Wandersee, James
2011-01-01
Dinosaurs in the middle school classroom can be exciting. These extinct reptiles are both an exotic subject and familiar to our students. Because students are inherently interested, dinosaurs can serve as an effective portal for the integration of biology, geology, ecology, and the history and nature of science. The field of dinosaur study is…
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PMG: online generation of high-quality molecular pictures and storyboarded animations
Autin, Ludovic; Tufféry, Pierre
2007-01-01
The Protein Movie Generator (PMG) is an online service able to generate high-quality pictures and animations for which one can then define simple storyboards. The PMG can therefore efficiently illustrate concepts such as molecular motion or formation/dissociation of complexes. Emphasis is put on the simplicity of animation generation. Rendering is achieved using Dino coupled to POV-Ray. In order to produce highly informative images, the PMG includes capabilities of using different molecular representations at the same time to highlight particular molecular features. Moreover, sophisticated rendering concepts including scene definition, as well as modeling light and materials are available. The PMG accepts Protein Data Bank (PDB) files as input, which may include series of models or molecular dynamics trajectories and produces images or movies under various formats. PMG can be accessed at http://bioserv.rpbs.jussieu.fr/PMG.html. PMID:17478496
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NASA Astrophysics Data System (ADS)
Romo, Jaime E., Jr.
Optical microscopy, the most common technique for viewing living microorganisms, is limited in resolution by Abbe's criterion. Recent microscopy techniques focus on circumnavigating the light diffraction limit by using different methods to obtain the topography of the sample. Systems like the AFM and SEM provide images with fields of view in the nanometer range with high resolvable detail, however these techniques are expensive, and limited in their ability to document live cells. The Dino-Lite digital microscope coupled with the Zeiss Axiovert 25 CFL microscope delivers a cost-effective method for recording live cells. Fields of view ranging from 8 microns to 300 microns with fair resolution provide a reliable method for discovering native cell structures at the nanoscale. In this report, cultured HeLa cells are recorded using different optical configurations resulting in documentation of cell dynamics at high magnification and resolution.
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NASA Astrophysics Data System (ADS)
Antonella Dino, Giovanna; Willy Danielsen, Svein; Chiappino, Claudia; Primavori, Piero; Engelsen, Christian John
2016-04-01
Resource preservation is one of the main challenges in Europe, together with waste management and recycling; recently several researchers are interested in the recovering of critical raw materials and secondary raw materials from landfill. Aggregate supply, even if it is not "critical" sensus stricto (s.s.), is one of the European priorities (low value but high volume needs). On the other side, the management of quarry waste , mainly from dimension stones, but also as fines from aggregate crushing, is still a matter of concern. Such materials are managed in different ways both locally and nationwide, and often they are landfilled, because of an unclear legislation and a general lack of data. Most of time the local authorities adopt the maximum precaution principle or the enterprises find it little profitable to recover them, so that the sustainable recycling of such material is not valued. Several studies have shown, depending on the material specific characteristics, the viability of recycling quarry waste into new raw materials used in glass and ceramic industries, precast concrete production, infrastructures etc. (Loudes et al. 2012, Dino&Marian 2015, Bozzola et al 2012, Dino et al. 2012, etc.). Thus, aggregate production may be one of the profitable ways to use quarry waste and is falling under the priority of EU (aggregate supply). Positive economic and environmental effects are likely to be achieved by systematic recycling of quarry waste planned by industries (industrial planning) and public authorities (national and local planning of aggregate exploitation). Today, the recycling level varies to a great extent and systematic recovery is not common among European Countries. In Italy and Norway no significant incentives on recycling or systematic approaches for local aggregate exploitation exist. The environmental consequences can be overexploitation of the natural resources, land take for the landfills, environmental contamination and landscape alteration by
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Comparison among cognitive diagnostic models for the TIMSS 2007 fourth grade mathematics assessment.
Yamaguchi, Kazuhiro; Okada, Kensuke
2018-01-01
A variety of cognitive diagnostic models (CDMs) have been developed in recent years to help with the diagnostic assessment and evaluation of students. Each model makes different assumptions about the relationship between students' achievement and skills, which makes it important to empirically investigate which CDMs better fit the actual data. In this study, we examined this question by comparatively fitting representative CDMs to the Trends in International Mathematics and Science Study (TIMSS) 2007 assessment data across seven countries. The following two major findings emerged. First, in accordance with former studies, CDMs had a better fit than did the item response theory models. Second, main effects models generally had a better fit than other parsimonious or the saturated models. Related to the second finding, the fit of the traditional parsimonious models such as the DINA and DINO models were not optimal. The empirical educational implications of these findings are discussed.
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Comparison among cognitive diagnostic models for the TIMSS 2007 fourth grade mathematics assessment
Okada, Kensuke
2018-01-01
A variety of cognitive diagnostic models (CDMs) have been developed in recent years to help with the diagnostic assessment and evaluation of students. Each model makes different assumptions about the relationship between students’ achievement and skills, which makes it important to empirically investigate which CDMs better fit the actual data. In this study, we examined this question by comparatively fitting representative CDMs to the Trends in International Mathematics and Science Study (TIMSS) 2007 assessment data across seven countries. The following two major findings emerged. First, in accordance with former studies, CDMs had a better fit than did the item response theory models. Second, main effects models generally had a better fit than other parsimonious or the saturated models. Related to the second finding, the fit of the traditional parsimonious models such as the DINA and DINO models were not optimal. The empirical educational implications of these findings are discussed. PMID:29394257
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Novel insight into the role of heterotrophic dinoflagellates in the fate of crude oil in the sea.
Almeda, Rodrigo; Connelly, Tara L; Buskey, Edward J
2014-12-19
Although planktonic protozoans are likely to interact with dispersed crude oil after a spill, protozoan-mediated processes affecting crude oil pollution in the sea are still not well known. Here, we present the first evidence of ingestion and defecation of physically or chemically dispersed crude oil droplets (1-86 μm in diameter) by heterotrophic dinoflagellates, major components of marine planktonic food webs. At a crude oil concentration commonly found after an oil spill (1 μL L(-1)), the heterotrophic dinoflagellates Noctiluca scintillans and Gyrodinium spirale grew and ingested ~0.37 μg-oil μg-C(dino)(-1) d(-1), which could represent ~17% to 100% of dispersed oil in surface waters when heterotrophic dinoflagellates are abundant or bloom. Egestion of faecal pellets containing crude oil by heterotrophic dinoflagellates could contribute to the sinking and flux of toxic petroleum hydrocarbons in coastal waters. Our study indicates that crude oil ingestion by heterotrophic dinoflagellates is a noteworthy route by which petroleum enters marine food webs and a previously overlooked biological process influencing the fate of crude oil in the sea after spills.
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NASA Astrophysics Data System (ADS)
Koutavas, A.; Dimitrakopoulos, A. P.
2015-12-01
We present a 750-year long tree-ring chronology from black pines (Pinus nigra) in Valia Kalda National Park, Pindos Mountains, Greece. The chronology shows a strong climate signal which consists of significant negative correlation (R=-0.5) with summer temperature (Jun-Jul-Aug-Sep), and positive correlation with summer precipitation. We exploit these relationships to reconstruct summer climate from ~1250 CE to present. In particular we investigate the character of the Little Ice Age (LIA) on mountainous Greece. We find evidence for cooler/wetter summers during the 18th and 19th centuries, but warmer/drier summers during the 14th through 17th centuries, during some of the coldest periods of the LIA in Northern Europe including the Maunder Minimum. This counter-intuitive pattern suggests the LIA had distinct signatures in the Easter Mediterranean, diverging from those of Northern Europe. The temperature pattern reconstructed here is remarkably similar to a recent reconstruction of summer temperatures from maximum latewood density (MXD) of Pinus heldreichii on Mount Olympus, just 150 km east of our site. However, because of the ambivalence of the climate signal with respect to temperature vs. precipitation in both of these reconstructions, there remains uncertainty as to whether the LIA was primarily warm, or dry, or some combination. We advocate for further reconstructions of LIA climate in the Balkan Peninsula and Eastern Mediterranean to explore relationships with Northern Europe and elucidate the broader climatic pattern and dynamical connections.
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Cardozo, Darío; Toledo, Luís Felipe
2013-11-08
Pseudopaludicola riopiedadensis was described by Mercadal de Barrio and Barrio (1994) based on two adult females collected by Luiz Dino Vizotto in 1963 from Rio Piedade, São José do Rio Preto, São Paulo, Brazil. This taxon was differentiated from P. ternetzi based on a series of qualitative and morphometric characters. Nevertheless, the original description and the type material of P. ternetzi were not considered by Mercadal de Barrio and Barrio, and the morphological variation of P. ternetzi was not documented. This work reviews the sample collected by Vizotto in P. riopiedadensis type locality, evaluates the advertisement calls obtained from such population, the two vouchers assigned to P. riopiedadensis, and a large data set, including type specimens of P. ternetzi to document the morphological variation along its known distribution. Results indicate that P. riopiedadensis was described on the basis of highly variable characters applied to a small sample and share the unique P. ternetzi autapomorphy, a robust body structure with immaculate belly. The lack of differentiation in both advertisement call and morphology rejects the status of P. riopiedadensis as distinct species, and we therefore suggest to formally consider P. riopiedadensis as junior synonym of P. ternetzi.
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Adverse risk: a 'dynamic interaction model of patient moving and handling'.
Griffiths, Howard
2012-09-01
The aim of the present study was to examine patient adverse events associated with sub-optimal patient moving and handling. Few studies have examined the patient's perspective on adverse risk during manual handling episodes. A narrative review was undertaken to develop the 'Dynamic Interaction Model of Patient Moving and Handling' in an orthopaedic rehabilitation setting, using peer-reviewed publications published in English between 1992 and 2010. Five predominant themes emerged from the narrative review: 'patient's need to know about analgesics prior to movement/ambulation'; 'comfort care'; 'mastery of and acceptance of mobility aids/equipment'; 'psychological adjustment to fear of falling'; and 'the need for movement to prevent tissue pressure damage'. Prevalence of discomfort, pain, falls, pressure sores together with a specific Direct Instrument Nursing Observation (DINO) tool enable back care advisers to measure quality of patient manual handling. Evaluation of patients' use of mobility aids together with fear of falling may be important in determining patients' recovery trajectory. Clinical governance places a responsibility on nurse managers to consider quality of care for their service users. 'Dynamic Interaction Model of Nurse-Patient Moving and Handling' provides back care advisers, clinical risk managers and occupational health managers with an alternative perspective to clinical risk and occupational risk. © 2011 Blackwell Publishing Ltd.
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Abassi, Sofia; Wang, Hui; Park, Bum Soo; Park, Jong-Woo; Ki, Jang-Seu
2017-01-01
The marine dinoflagellate Cochlodinium polykrikoides is one of the most common ichthyotoxic species that causes harmful algal blooms (HABs), which leads to ecological damage and huge economic loss in aquaculture industries. Cyclophilins (CYPs) belong to the immunophilin superfamily, and they may play a role in the survival mechanisms of the dinoflagellate in stress environments. In the present study, we identified a novel cyclophilin gene from C. polykrikoides and examined physiological and gene transcriptional responses to biocides copper sulphate (CuSO 4 ) and sodium hypochlorite (NaOCl). The full length of CpCYP was 903 bp, ranging from the dinoflagellate splice leader (DinoSL) sequence to the polyA tail, comprising a 639 bp ORF, a 117 bp 5'-UTR, and a 147 bp 3'-UTR. Motif and phylogenetic comparisons showed that CpCYP was affiliated to group B of CYP. In biocide stressors, cell counts, chlorophyll a , and photosynthetic efficiency ( Fv / Fm ) of C. polykrikoides were considerably decreased in both exposure time- and dose-dependent manners. In addition, CpCYP gene expression was significantly induced after 24 h exposure to the biocide-treated stress conditions. These results indicate an effect of the biocides on the cell physiology and expression profile of CpCYP , suggesting that the gene may play a role in environmental stress responses.
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Self-Trail, Jean M.; Edwards, Lucy E.; Litwin, Ronald J.
2009-01-01
Biostratigraphic analysis of sedimentary breccias and diamictons in the Chesapeake Bay impact structure provides information regarding the timing and processes of late-stage gravitational crater collapse and ocean resurge. Studies of calcareous nannofossil and palynomorph assemblages in the International Continental Scientific Drilling Program (ICDP)–U.S. Geological Survey (USGS) Eyreville A and B cores show the mixed-age, mixed-preservation microfossil assemblages that are typical of deposits from the upper part of the Chesapeake Bay impact structure. Sparse, poorly preserved, possibly thermally altered pollen is present within a gravelly sand interval below the granite slab at 1392 m in Eyreville core B, an interval that is otherwise barren of calcareous nannofossils and dinocysts. Gravitational collapse of water- saturated sediments from the transient crater wall resulted in the deposition of sediment clasts primarily derived from the nonmarine Cretaceous Potomac Formation. Collapse occurred before the arrival of resurge. Low pollen Thermal Alteration Index (TAI) values suggest that these sediments were not thermally altered by contact with the melt sheet. The arrival of resurge sedimentation is identified based on the presence of diamicton zones and stringers rich in glauconite and marine microfossils at 866.7 m. This horizon can be traced across the crater and can be used to identify gravitational collapse versus ocean-resurge sedimentation. Glauconitic quartz sand diamicton dominates the sediments above 618.2 m. Calcareous nannofossil and dino-flagellate data from this interval suggest that the earliest arriving resurge from the west contained little or no Cretaceous marine input, but later resurge pulses mined Cretaceous sediments east of the Watkins core in the annular trough. Additionally, the increased distance traveled by resurge to the central crater in turbulent flow conditions resulted in the disaggregation of Paleogene unconsolidated sediments. As
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Völler, Swantje; Flint, Robert B; Stolk, Leo M; Degraeuwe, Pieter L J; Simons, Sinno H P; Pokorna, Paula; Burger, David M; de Groot, Ronald; Tibboel, Dick; Knibbe, Catherijne A J
2017-11-15
Particularly in the pediatric clinical pharmacology field, data-sharing offers the possibility of making the most of all available data. In this study, we utilize previously collected therapeutic drug monitoring (TDM) data of term and preterm newborns to develop a population pharmacokinetic model for phenobarbital. We externally validate the model using prospective phenobarbital data from an ongoing pharmacokinetic study in preterm neonates. TDM data from 53 neonates (gestational age (GA): 37 (24-42) weeks, bodyweight: 2.7 (0.45-4.5) kg; postnatal age (PNA): 4.5 (0-22) days) contained information on dosage histories, concentration and covariate data (including birth weight, actual weight, post-natal age (PNA), postmenstrual age, GA, sex, liver and kidney function, APGAR-score). Model development was carried out using NONMEM ® 7.3. After assessment of model fit, the model was validated using data of 17 neonates included in the DINO (Drug dosage Improvement in NeOnates)-study. Modelling of 229 plasma concentrations, ranging from 3.2 to 75.2mg/L, resulted in a one compartment model for phenobarbital. Clearance (CL) and volume (V d ) for a child with a birthweight of 2.6kg at PNA day 4.5 was 0.0091L/h (9%) and 2.38L (5%), respectively. Birthweight and PNA were the best predictors for CL maturation, increasing CL by 36.7% per kg birthweight and 5.3% per postnatal day of living, respectively. The best predictor for the increase in V d was actual bodyweight (0.31L/kg). External validation showed that the model can adequately predict the pharmacokinetics in a prospective study. Data-sharing can help to successfully develop and validate population pharmacokinetic models in neonates. From the results it seems that both PNA and bodyweight are required to guide dosing of phenobarbital in term and preterm neonates. Copyright © 2017 The Authors. Published by Elsevier B.V. All rights reserved.
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Cok, S J; Martin, C G; Gordon, J I
1998-01-01
Inositol regulates transcription of Saccharomyces cerevisiae genes required for de novo synthesis of acylCoAs and phospholipids. Removal of inositol results in transcriptional activation by heterodimeric complexes of two bHLH proteins, Ino2p and Ino4p. In the presence of inositol, transcription is repressed by Opi1p. MyristoylCoA:protein N-myristoyltransferase (Nmt1p) is an essential enzyme whose activity is influenced by cellular myristoylCoA pool size and availability. nmt451Dp contains a Gly451-->Asp substitution that produces temperature-dependent reductions in affinity for myristoylCoA and associated reductions in acylation of cellular N-myristoylproteins. The conditional lethality produced by nmt1-451D is rescued at temperatures up to 33 degreesC by withdrawal of inositol. We tested the hypothesis that N-myristoylproteins function to regulate INO2, INO4 and/or OPI1 transcription, thereby affecting the expression of inositol-sensitive genes that influence myristoylCoA metabolism. The effect of nmt1-451D on INO2 , INO4 and OPI1 promoter activities was examined by introducing episomes, containing their 5' non-transcribed domains linked to reporters, into isogenic NMT1 and nmt1-451D cells. The activity of INO2 is significantly higher, INO4 significantly lower and OPI1 unaffected in nmt1-451D cells, both in the presence and absence of inositol. These changes are associated with a net increase in expression of some inositol target genes, including FAS1 . FAS1 encodes one of the subunits of the fatty acid synthase complex that catalyzes de novo acylCoA (including myristoylCoA) biosynthesis. Augmented expression of FAS1 overcomes the kinetic defects in nmt451Dp. FAS1 expression is Ino2p-dependent in NMT1 cells at 24-33 degreesC. In contrast, FAS1 expression becomes Ino2p-independent in nmt1-451D cells at temperatures where efficient acylation of cellular N-myristoylproteins is jeopardized. The ability to maintain expression of FAS1 in nmt1-451Dino2 Delta cells
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The Use of Technology in Identifying Hospital Malnutrition: Scoping Review.
Trtovac, Dino; Lee, Joon
2018-01-19
already malnourished and those who are at risk of malnourishment. Studies went on to examine the effectiveness of health care workers (nurses and doctors) with a knowledge base focused on clinical care and their ability to accurately and consistently identify malnourished geriatric patients within that setting. Most articles reported effectiveness in accurately increasing malnutrition detection and awareness. Computerized tools and apps may also help reduce health care workers' workload and time spent assessing patients for malnutrition. Hospitals may also benefit from implementing malnutrition technology through observing decreased length of stay, along with decreased foregone costs related to missing malnutrition diagnoses. It is beneficial to study the impact of these technologies to examine possible areas of improvement. A future systematic review would further contribute to the evidence and effectiveness of the use of technologies in assessing and monitoring hospital malnutrition. ©Dino Trtovac, Joon Lee. Originally published in JMIR Medical Informatics (http://medinform.jmir.org), 19.01.2018.
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Scott, Michael L; Friedman, Jonathan M.
2018-01-01
This report addresses the relation between flow of the Yampa River and occurrence of herbaceous and woody riparian vegetation in Dinosaur National Monument (DINO) with the goal of informing management decisions related to potential future water development. The Yampa River in DINO flows through diverse valley settings, from the relatively broad restricted meanders of Deerlodge Park to narrower canyons, including debris fan-affected reaches in the upper Yampa Canyon and entrenched meanders in Harding Hole and Laddie Park. Analysis of occurrence of all plant species measured in 1470 quadrats by multiple authors over the last 24 years shows that riparian vegetation along the Yampa River is strongly related to valley setting and geomorphic surfaces, defined here as active channel, active floodplain, inactive floodplain, and upland. Principal Coordinates Ordination arrayed quadrats and species along gradients of overall cover and moisture availability, from upland and inactive floodplain quadrats and associated xeric species like western wheat grass (Pascopyrum smithii), cheatgrass (Bromus tectorum), and saltgrass (Distichlis spicata) to active channel and active floodplain quadrats supporting more mesic species including sandbar willow (Salix exigua), wild licorice (Glycyrrhiza lepidota), and cordgrass (Spartina spp.). Indicator species analysis identified plants strongly correlated with geomorphic surfaces. These species indicate state changes in geomorphic surfaces, such as the conversion of active channel to floodplain during channel narrowing. The dominant woody riparian species along the Yampa River are invasive tamarisk (Tamarix ramosissima), and native Fremont cottonwood (Populus deltoides ssp. wislizenii), box elder (Acer negundo L. var. interius), and sandbar willow (Salix exigua). These species differ in tolerance of drought, salinity, inundation, flood disturbance and shade, and in seed size, timing of seed dispersal and ability to form root sprouts. These
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NASA Astrophysics Data System (ADS)
2011-07-01
(Orsay) S Hamann (Bochum)R Varga (Košice) U Hannemann (Dresden)P Vavassori (San Sebastian) L Havela (Prague)W Wulfhekel (Karlsruhe) O Heczko (Prague)M Yamashita (Sendai) B Hernando (Oviedo)R Zdyb (Lublin) O Isnard (Grenoble)A Zhukov (San Sebastián) Z Kąkol (Kraków)A K Zvezdin (Moscow) N-T H Kim-Ngan (Kraków) International Advisory Committee (2011): Dominique Givord, President (Grenoble)Ludwig Schultz, Former President (Dresden) Manfred Albrecht (Chemnitz)Burkard Hillebrands (Kaiserslautern) Agnés Barthélémy (Paris)Andrei Kirilyuk (Nijmegen) Roy Chantrell (York)Ron Jansen (Tsukuba) Russell Cowburn (London)Nicoleta Lupu (Iasi) Tomasz Dietl (Warszawa)Caroline A Ross (Cambridge, MA) Claudia Felser (Mainz)Stefano Sanvito (Dublin) Josef Fidler (Wien)Vladimir Sechovsky (Praha) Dino Fiorani (Roma)Roberta Sessoli (Firenze) Pietro Gambardella (Bellaterra)Jozef Spałek (Kraków) Alberto Guimarães (Rio de Janeiro)
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NASA Astrophysics Data System (ADS)
Scapozza, Cristian; Bruder, Andreas; Domenici, Mattia; Lepori, Fabio; Pera, Sebastian; Pozzoni, Maurizio; Rioggi, Stefano; Colombo, Luca
2017-04-01
vegetation). First results suggest that Lago Nero is particularly sensitive to changes in the cryosphere, particularly concerning thickness of snow cover, snowmelt date and duration, and length of ice-free period of the lake surface. Probable storage of ground ice during the 1966-1985 period (deduced from the nearby Basòdino Glacier) and its significant melting in the last decades may explain the high amounts of sulphur measured in the outflow of the rock glacier. High levels of sulphur are likely to have ecological effects on the sensitive biota of the Lago Nero catchment, for instance by retarding the recovery from past acidification. REFERENCES Bruder A., Lepori F., Pozzoni M., Pera S., Scapozza C., Rioggi S., Domenici M. & Colombo L. (2016). Lago Nero - a new site to assess the effects of environmental change on high-alpine lakes and their catchments. In: S. Kleemola & M. Forsius (eds.), 25th Annual Report 2016. Convention on Long-range transboundary air pollution. Reports of the Finnish Environments Institute 29: 52-56. Scapozza C. & Mari S. (2010). Catasto, caratteristiche e dinamica dei rock glacier delle Alpi Ticinesi. Bollettino della Società ticinese di Scienze naturali 98: 15-29. [http://repository.supsi.ch/2152/
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EDITORIAL: Focus on Micro- and Nanofluidics FOCUS ON MICRO- AND NANOFLUIDICS
NASA Astrophysics Data System (ADS)
Ajdari, Armand; Stone, Howard A.
2009-07-01
-aspect-ratio microchannels Aman Russom, Amit K Gupta, Sunitha Nagrath, Dino Di Carlo, Jon F Edd and Mehmet Toner Capillary instability on a hydrophilic stripe Raymond L Speth and Eric Lauga Universal nanocolloid deposition patterns: can you see the harmonics of a Taylor cone? Xinguang Cheng and Hsueh-Chia Chang Osmotic manipulation of particles for microfluidic applications B Abécassis, C Cottin-Bizonne, C Ybert, A Ajdari and L Bocquet Scaling the drop size in coflow experiments E Castro-Hernández, V Gundabala, A Fernández-Nieves and J M Gordillo Pattern formation during the evaporation of a colloidal nanoliter drop: a numerical and experimental study Rajneesh Bhardwaj, Xiaohua Fang and Daniel Attinger Topology and shape optimization of induced-charge electro-osmotic micropumps M M Gregersen, F Okkels, M Z Bazant and H Bruus Fabrication of multiphasic and regio-specifically functionalized PRINT® particles of controlled size and shape H Zhang, J K Nunes, S E A Gratton, K P Herlihy, P D Pohlhaus and J M DeSimone Using TIRF microscopy to quantify and confirm efficient mass transfer at the substrate surface of the chemistrode Delai Chen, Wenbin Du and Rustem F Ismagilov Nonlinear electrokinetics at large voltages Martin Z Bazant, Mustafa Sabri Kilic, Brian D Storey and Armand Ajdari Interdiffusion of liquids of different viscosities in a microchannel J Dambrine, B Géraud and J-B Salmon Microfluidic fabrication of microparticles with structural complexity using photocurable emulsion droplets Shin-Hyun Kim, Jae Won Shim, Jong-Min Lim, Su Yeon Lee and Seung-Man Yang
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The Dark Side of Nature: the Crime was Almost Perfect
NASA Astrophysics Data System (ADS)
2006-12-01
Astrofisico di Arcetri, Italy), Guido Chincarini (INAF, Osservatorio Astronomico di Brera & Università degli Studi di Milano-Bicocca, Italy), Nino Panagia (Space Telescope Science Institute, USA), Gianpiero Tagliaferri, Dino Fugazza, Sergio Campana, Stefano Covino, and Paolo D'Avanzo (INAF, Osservatorio Astronomico di Brera, Italy), Daniele Malesani (SISSA/ISAS, Italy and Dark Cosmology Centre, Copenhagen), Vincenzo Testa, L. Angelo Antonelli, Silvia Piranomonte, and Luigi Stella (INAF, Osservatorio Astronomico di Roma, Italy), Vanessa Mangano (INAF/IASF Palermo, Italy), Kevin Hurley (University of California, Berkeley, USA), I. Felix Mirabel (ESO), and Leonardo J. Pellizza (Instituto de Astronomia y Fisica del Espacio). The Danish-led team is composed of Johan P. U. Fynbo, Darach Watson, Christina C. Thöne, Tamara M. Davis, Jens Hjorth, José Mará Castro Cerón, Brian L. Jensen, Maximilian D. Stritzinger, and Dong Xu (Dark Cosmology Centre, University of Copenhagen, Denmark), Jesper Sollerman (Dark Cosmology Centre and Department of Astronomy, Stockholm University, Sweden), Uffe G. Jørgensen, Tobias C. Hinse, and Kristian G. Woller (Niels Bohr Institute, University of Copenhagen), Joshua S. Bloom, Daniel Kocevski, Daniel Perley (Department of Astronomy, University of California at Berkeley, USA), Páll Jakobsson (Centre for Astrophysics Research, University of Hertfordshire, UK), John F. Graham and Andrew S. Fruchter (Space Telescope Science Institute, Baltimore, USA), David Bersier (Astrophysics Research Institute, Liverpool John Moores University, UK), Lisa Kewley (University of Hawaii, Institute of Astronomy, USA), Arnaud Cassan and Marta Zub (Astronomisches Rechen-Institut, Zentrum für Astronomie der Universität Heidelberg, Germany), Suzanne Foley (School of Physics, University College Dublin, Ireland), Javier Gorosabel (Instituto de Astrofisica de Andalucia, Granada, Spain), Keith D. Horne (SUPA Physics/Astronomy, University of St Andrews, Scotland, UK), Sylvio