Sample records for kazlauskas tiit randveer
-
Multicolor CCD Photometry of the Open Cluster IC361
2010-01-01
journal Volume 19 Numbers 1/2 2010 Contents V. Straizys, A. Kazlauskas. Young stars in the Camelopardalis dust and molecular clouds. VI. YSOs...Vilnius + I system for 7250 stars down to 1= 19.6 mag has been obtained in the 20’ x 26’ field of the open cluster IC 361 in Camelopardalis . The catalog...1= 19.6 mag has been obtained in the 20’ x 26’ field of the open cluster IC 361 in Camelopardalis . The catalog of 1420 stars down to V ~ 18.5 mag
-
2013-06-11
... 11'' Mev 'll :lf: i! r ill \\\\ I) i! !! Com ;i.;ir i;; cnoi ini" I. ;i I ii n dl PCB da I a I: r:; inn t hn: ee con I 11 ;; 11 n in i; ... 6 C o in |i ii i: .i !• on and Quiiiiili tiit l ive ...
-
NASA Astrophysics Data System (ADS)
Nik Him, N. R.; Ibrahim, D.
2018-05-01
In our previous work, a new lipase enzyme has been purified from a species identified as a Gram negative Geobacillus thermodenitrificans nr68, isolated from a hot spring in Malaysia with growth temperature of 48°C. This new lipase, called Lip.nr-68 has been characterized as a hyperthermotolerant protein with high stability at 65°C and has been showing excellent characteristics that are very much comparable yet better than some of those of well-known industrially-used lipases. It shows high activity against long-chain triglycerides with molecular weight of the purified enzyme estimated to be 33.5 kDa using SDS-PAGE analysis. This paper is focusing on hyperthermotolerant Lip.nr-68 performance in promoting for enantioselectivity activities towards three secondary racemic alcohols namely 1-phenylethanol, 1-cyclohexilethanol and 1-(naft-2-il) ethanol by acetylation with vinyl acetate. Lip.nr-68 has been confirmed to show high and usual enantioselectivitiy according to the Kazlauskas Rule towards all secondary racemic alcohols and has significantly approved as an enantiomer selective biocatalyst towards 1-phenylethanol and 1-cyclohexylethanol at 65°C. Lip.nr-68 has showed a reduction of (R) and (S) enantiomers as well as the production of 68-98% ee and almost 94% yield of 3-4 mg/ml for 1-cyclohexilethanol.
-
Pereira, Mariana Rangel; Mercaldi, Gustavo Fernando; Maester, Thaís Carvalho; Balan, Andrea; Lemos, Eliana Gertrudes de Macedo
2015-01-01
Lipolytic enzymes have attracted attention from a global market because they show enormous biotechnological potential for applications such as detergent production, leather processing, cosmetics production, and use in perfumes and biodiesel. Due to the intense demand for biocatalysts, a metagenomic approach provides methods of identifying new enzymes. In this study, an esterase designated as Est16 was selected from 4224 clones of a fosmid metagenomic library, revealing an 87% amino acid identity with an esterase/lipase (accession number ADM63076.1) from an uncultured bacterium. Phylogenetic studies showed that the enzyme belongs to family V of bacterial lipolytic enzymes and has sequence and structural similarities with an aryl-esterase from Pseudomonas fluorescens and a patented Anti-Kazlauskas lipase (patent number US20050153404). The protein was expressed and purified as a highly soluble, thermally stable enzyme that showed a preference for basic pH. Est16 exhibited activity toward a wide range of substrates and the highest catalytic efficiency against p-nitrophenyl butyrate and p-nitrophenyl valerate. Est16 also showed tolerance to the presence of organic solvents, detergents and metals. Based on molecular modeling, we showed that the large alpha-beta domain is conserved in the patented enzymes but not the substrate pocket. Here, it was demonstrated that a metagenomic approach is suitable for discovering the lipolytic enzyme diversity and that Est16 has the biotechnological potential for use in industrial processes.
-
COLOR OF THE STARS: Oh Be A Fine Girl, Kiss Me!
NASA Astrophysics Data System (ADS)
Zambrano, L. F.; Boyle, R. P.; Janusz, R.; University-School "Ignatianum" Kracow, Poland Collaboration; A. G. Davis-InstituteSpace Observations Collaboration
2005-12-01
Classification of stars by color is important in stellar studies because from it we are able to attain essential information about stars like: temperature, composition, age and mass; from these we can also derive its history, and future evolution!. This classification can be done by photometry or spectroscopy. Photometry provides information from more stars in a given field of view, magnitude and approximate size. The Strömvil photometric system, developed by V. Straizys (Vilnius Observatory, Lithuania), allows more precise photometry using 7 filters, ranging from 330-700 nm. Since the color of a star is associated with the wavelength of the electromagnetic radiation of light emitted by it, each filter allows only certain wavelengths to go through into the CCD camera; then, each neighboring wavelength band can be compared against the others and the color relationship can be converted to magnitude. Our Milky Way galaxy has billions of stars, of which we only have information from a small set. We obtained images of the NGC6811 and NGC6819 Open clusters, and the M56 Globular cluster at the Vatican Advanced Technology Telescope in Mt Graham AZ. During an 8 night observing run, images were taken in each filter with 3 different pointings overlapping by 2 arc-min. Calibration by known standards from A. Kazlauskas (e.i. Baltic Astronomy Vol II) that fall in the observed regions will be done. From this photometry other star information; such as luminosity, distance, metallicity, surface gravity, and spectral class will be determined.
-
Chronic neck pain in young adults: perspectives on anatomic differences.
Lee, Ji-Hye; Park, Youn-Kwan; Kim, Joo-Han
2014-11-01
Neck pain (NP) is a common musculoskeletal disorder, but little is known about the associated risk factors. We compared anatomic differences in the neck and trunk area of young adult patients with chronic NP and control subjects without NP to identify risk factors and predictors. This is an age-, sex-, and body mass index-matched retrospective case-control study of a consecutive sample. Patients with axial NP for longer than 6 months (23 males and 25 females) and pain-free volunteers (23 males and 25 females) were included. Outcome measures were linear and angular dimensions of the cervicothoracic junction. Midsagittal magnetic resonance imaging scans of the cervicothoracic spine were obtained. Four linear and four angular parameters were identified and measured. These parameters included depth of the T1-manubrium arch (T1AD), depth of the thoracic cage (TXD), tangential height of T1 (T1H1), relative height of T1 (T1H2), T1 slope (T1S), thoracic inlet inclination (TiI), T1-manubrium arch inclination (T1AI), and the angular difference between TiI and T1AI (TiI-T1AI). The measurements were taken by two neurosurgeons. Depth of the T1-manubrium arch and TiI were identified as predictors for NP in the binary logistic regression analysis. Each millimeter increase in T1AD lessened the probability of NP with an adjusted odds ratio (OR) of 0.823 (95% confidence interval [CI], 0.701-0.966) in females and 0.809 (95% CI, 0.681-0.959) in males. Each degree increase in TiI was associated with the probability of NP with an adjusted OR of 1.247 (95% CI, 1.060-1.466) in males. Measurement of cervicothoracic junctional structures is a reliable and feasible method of estimating potential predictor of chronic NP in young adults. Forward inclination of the thoracic inlet in males and a shallow thoracic cage in females were identified as important predictors. Copyright © 2014 Elsevier Inc. All rights reserved.