A bio-image sensor for simultaneous detection of multi-neurotransmitters.
Lee, You-Na; Okumura, Koichi; Horio, Tomoko; Iwata, Tatsuya; Takahashi, Kazuhiro; Hattori, Toshiaki; Sawada, Kazuaki
2018-03-01
We report here a new bio-image sensor for simultaneous detection of spatial and temporal distribution of multi-neurotransmitters. It consists of multiple enzyme-immobilized membranes on a 128 × 128 pixel array with read-out circuit. Apyrase and acetylcholinesterase (AChE), as selective elements, are used to recognize adenosine 5'-triphosphate (ATP) and acetylcholine (ACh), respectively. To enhance the spatial resolution, hydrogen ion (H + ) diffusion barrier layers are deposited on top of the bio-image sensor and demonstrated their prevention capability. The results are used to design the space among enzyme-immobilized pixels and the null H + sensor to minimize the undesired signal overlap by H + diffusion. Using this bio-image sensor, we can obtain H + diffusion-independent imaging of concentration gradients of ATP and ACh in real-time. The sensing characteristics, such as sensitivity and detection of limit, are determined experimentally. With the proposed bio-image sensor the possibility exists for customizable monitoring of the activities of various neurochemicals by using different kinds of proton-consuming or generating enzymes. Copyright © 2017 Elsevier B.V. All rights reserved.
Multivariable bio-inspired photonic sensors for non-condensable gases
NASA Astrophysics Data System (ADS)
Potyrailo, Radislav A.; Karker, Nicholas; Carpenter, Michael A.; Minnick, Andrew
2018-02-01
Existing gas sensors often lose their measurement accuracy in practical field applications. To mitigate this significant problem, here, we report a demonstration of fabricated multivariable photonic sensors inspired by a known nanostructure of Morpho butterfly scales for detection of exemplary non-condensable gases such as H2, CO, and CO2. We fabricated bio-inspired nanostructures using conventional photolithography and chemical etching and detected individual gases that were difficult or unrealistic to detect using natural Morpho nanostructures. Such bio-inspired gas sensors are the critical step in the development of new sensors with improved accuracy for diverse operational scenarios. While this report is our initial demonstration of responses of fabricated multivariable sensors to individual gases in pristine laboratory conditions, it is a significant milestone in understanding the next steps toward field tests and practical applications of these sensors.
Bio-Mimetic Sensors Based on Molecularly Imprinted Membranes
Algieri, Catia; Drioli, Enrico; Guzzo, Laura; Donato, Laura
2014-01-01
An important challenge for scientific research is the production of artificial systems able to mimic the recognition mechanisms occurring at the molecular level in living systems. A valid contribution in this direction resulted from the development of molecular imprinting. By means of this technology, selective molecular recognition sites are introduced in a polymer, thus conferring it bio-mimetic properties. The potential applications of these systems include affinity separations, medical diagnostics, drug delivery, catalysis, etc. Recently, bio-sensing systems using molecularly imprinted membranes, a special form of imprinted polymers, have received the attention of scientists in various fields. In these systems imprinted membranes are used as bio-mimetic recognition elements which are integrated with a transducer component. The direct and rapid determination of an interaction between the recognition element and the target analyte (template) was an encouraging factor for the development of such systems as alternatives to traditional bio-assay methods. Due to their high stability, sensitivity and specificity, bio-mimetic sensors-based membranes are used for environmental, food, and clinical uses. This review deals with the development of molecularly imprinted polymers and their different preparation methods. Referring to the last decades, the application of these membranes as bio-mimetic sensor devices will be also reported. PMID:25196110
Novel Bio-inspired Aquatic Flow Sensors
2012-06-18
Novel Bio-inspired Aquatic Flow Sensors Preston Albert Pinto Thesis submitted to the Faculty of the Virginia Polytechnic Institute and...Leo, Chair Stephen A. Sarles Michael K. Philen Pavlos Vlachos June 18th, 2012 Blacksburg, Virginia Keywords: artificial hair cell, flow ...Aquatic Flow Sensors 5a. CONTRACT NUMBER 5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) 5d. PROJECT NUMBER 5e. TASK NUMBER 5f. WORK UNIT
Aurelia aurita bio-inspired tilt sensor
NASA Astrophysics Data System (ADS)
Smith, Colin; Villanueva, Alex; Priya, Shashank
2012-10-01
The quickly expanding field of mobile robots, unmanned underwater vehicles, and micro-air vehicles urgently needs a cheap and effective means for measuring vehicle inclination. Commonly, tilt or inclination has been mathematically derived from accelerometers; however, there is inherent error in any indirect measurement. This paper reports a bio-inspired tilt sensor that mimics the natural balance organ of jellyfish, called the ‘statocyst’. Biological statocysts from the species Aurelia aurita were characterized by scanning electron microscopy to investigate the morphology and size of the natural sensor. An artificial tilt sensor was then developed by using printed electronics that incorporates a novel voltage divider concept in conjunction with small surface mount devices. This sensor was found to have minimum sensitivity of 4.21° with a standard deviation of 1.77°. These results open the possibility of developing elegant tilt sensor architecture for both air and water based platforms.
Characterizing the reliability of a bioMEMS-based cantilever sensor
NASA Astrophysics Data System (ADS)
Bhalerao, Kaustubh D.
2004-12-01
The cantilever-based BioMEMS sensor represents one instance from many competing ideas of biosensor technology based on Micro Electro Mechanical Systems. The advancement of BioMEMS from laboratory-scale experiments to applications in the field will require standardization of their components and manufacturing procedures as well as frameworks to evaluate their performance. Reliability, the likelihood with which a system performs its intended task, is a compact mathematical description of its performance. The mathematical and statistical foundation of systems-reliability has been applied to the cantilever-based BioMEMS sensor. The sensor is designed to detect one aspect of human ovarian cancer, namely the over-expression of the folate receptor surface protein (FR-alpha). Even as the application chosen is clinically motivated, the objective of this study was to demonstrate the underlying systems-based methodology used to design, develop and evaluate the sensor. The framework development can be readily extended to other BioMEMS-based devices for disease detection and will have an impact in the rapidly growing $30 bn industry. The Unified Modeling Language (UML) is a systems-based framework for design and development of object-oriented information systems which has potential application for use in systems designed to interact with biological environments. The UML has been used to abstract and describe the application of the biosensor, to identify key components of the biosensor, and the technology needed to link them together in a coherent manner. The use of the framework is also demonstrated in computation of system reliability from first principles as a function of the structure and materials of the biosensor. The outcomes of applying the systems-based framework to the study are the following: (1) Characterizing the cantilever-based MEMS device for disease (cell) detection. (2) Development of a novel chemical interface between the analyte and the sensor that provides a
Bio-Inspired Stretchable Absolute Pressure Sensor Network
Guo, Yue; Li, Yu-Hung; Guo, Zhiqiang; Kim, Kyunglok; Chang, Fu-Kuo; Wang, Shan X.
2016-01-01
A bio-inspired absolute pressure sensor network has been developed. Absolute pressure sensors, distributed on multiple silicon islands, are connected as a network by stretchable polyimide wires. This sensor network, made on a 4’’ wafer, has 77 nodes and can be mounted on various curved surfaces to cover an area up to 0.64 m × 0.64 m, which is 100 times larger than its original size. Due to Micro Electro-Mechanical system (MEMS) surface micromachining technology, ultrathin sensing nodes can be realized with thicknesses of less than 100 µm. Additionally, good linearity and high sensitivity (~14 mV/V/bar) have been achieved. Since the MEMS sensor process has also been well integrated with a flexible polymer substrate process, the entire sensor network can be fabricated in a time-efficient and cost-effective manner. Moreover, an accurate pressure contour can be obtained from the sensor network. Therefore, this absolute pressure sensor network holds significant promise for smart vehicle applications, especially for unmanned aerial vehicles. PMID:26729134
Microcavity surface plasmon resonance bio-sensors
NASA Astrophysics Data System (ADS)
Mosavian, Nazanin
This work discusses a miniature surface plasmon biosensor which uses a dielectric sub- micron diameter core with gold spherical shell. The shell has a subwavelength nanoaperture believed to excite stationary plasmon resonances at the biosensor's surface. The sub-micron cavity enhances the measurement sensitivity of molecules binding to the sensor surface. We used visible-range optical spectroscopy to study the wavelength shift as bio-molecules absorbed-desorbed at the shell surface. We also used Scanning Electron Microscopy (SEM) and Focused Ion Beam (FIB) ablation to study the characteristics of microcavity surface plasmon resonance sensor (MSPRS) and the inner structure formed with metal deposition and its spectrum. We found that resonances at 580 nm and 670 nm responded to bound test agents and that Surface Plasmon Resonance (SPR) sensor intensity could be used to differentiate between D-glucose and L-glucose. The responsiveness of the system depended upon the mechanical integrity of the metallic surface coating.
Optical bio-chemical sensors on SNOW ring resonators.
Khorasaninejad, Mohammadreza; Clarke, Nigel; Anantram, M P; Saini, Simarjeet Singh
2011-08-29
In this paper, we propose and analyze novel ring resonator based bio-chemical sensors on silicon nanowire optical waveguide (SNOW) and show that the sensitivity of the sensors can be increased by an order of magnitude as compared to silicon-on-insulator based ring resonators while maintaining high index contrast and compact devices. The core of the waveguide is hollow and allows for introduction of biomaterial in the center of the mode, thereby increasing the sensitivity of detection. A sensitivity of 243 nm/refractive index unit (RIU) is achieved for a change in bulk refractive index. For surface attachment, the sensor is able to detect monolayer attachments as small as 1 Å on the surface of the silicon nanowires.
Optical bio-chemical sensors on SNOW ring resonators
NASA Astrophysics Data System (ADS)
Khorasaninejad, Mohammadreza; Clarke, Nigel; Anantram, M. P.; Singh Saini, Simarjeet
2011-08-01
In this paper, we propose and analyze novel ring resonator based bio-chemical sensors on silicon nanowire optical waveguide (SNOW) and show that the sensitivity of the sensors can be increased by an order of magnitude as compared to silicon-on-insulator based ring resonators while maintaining high index contrast and compact devices. The core of the waveguide is hollow and allows for introduction of biomaterial in the center of the mode, thereby increasing the sensitivity of detection. A sensitivity of 243 nm/refractive index unit (RIU) is achieved for a change in bulk refractive index. For surface attachment, the sensor is able to detect monolayer attachments as small as 1 Å on the surface of the silicon nanowires.
pH measurements of FET-based (bio)chemical sensors using portable measurement system.
Voitsekhivska, T; Zorgiebel, F; Suthau, E; Wolter, K-J; Bock, K; Cuniberti, G
2015-01-01
In this study we demonstrate the sensing capabilities of a portable multiplex measurement system for FET-based (bio)chemical sensors with an integrated microfluidic interface. We therefore conducted pH measurements with Silicon Nanoribbon FET-based Sensors using different measurement procedures that are suitable for various applications. We have shown multiplexed measurements in aqueous medium for three different modes that are mutually specialized in fast data acquisition (constant drain current), calibration-less sensing (constant gate voltage) and in providing full information content (sweeping mode). Our system therefore allows surface charge sensing for a wide range of applications and is easily adaptable for multiplexed sensing with novel FET-based (bio)chemical sensors.
Cross-Discipline Bio-Nanostructured Enhanced Photonic Multimode-Sensor Science
2017-05-23
experimental study aimed to combine soft material science with nanotechnology and multi-physics modeling to produce adaptable bio-nanostructure based on...degradation through optical analysis and tracking programs Protein and DNA engineering . - The properties of proteins to be used in sensors were studies
Numerical Study on the Particle Trajectory Tracking in a Micro-UV Bio-Fluorescence Sensor.
Byeon, Sun-Seok; Cho, Moon-Young; Lee, Jong-Chul; Kim, Youn-Jea
2015-03-01
A micro-UV bio-fluorescence sensor was developed to detect primary biological aerosols including bacteria, bacterial spores, fungal spores, pollens, viruses, algae, etc. In order to effectively detect the bio-particles in a micro-UV bio-fluorescence sensor, numerical calculations were performed to adjust for appropriate flow conditions of the sensor by regulating the sample aerosols and sheath flow. In particular, a CFD-based model of hydrodynamic processes was developed by computing the trajectory of particles using commercially available ANSYS CFX-14 software and the Lagrangian tracking model. The established model was evaluated with regard to the variation of sheath flow rate and particle size. Results showed that the sheath flow was changed rapidly at the end of nozzle tip, but the sample particles moved near the center of aerosol jet for aerodynamic focusing with little deviation from the axis.
Chalcogenide glass sensors for bio-molecule detection
NASA Astrophysics Data System (ADS)
Lucas, Pierre; Coleman, Garrett J.; Cantoni, Christopher; Jiang, Shibin; Luo, Tao; Bureau, Bruno; Boussard-Pledel, Catherine; Troles, Johann; Yang, Zhiyong
2017-02-01
Chalcogenide glasses constitute the only class of materials that remain fully amorphous while exhibiting broad optical transparency over the full infrared region from 2-20 microns. As such, they can be shaped into complex optical elements while retaining a clear optical window that encompass the vibrational signals of virtually any molecules. Chalcogenide glasses are therefore ideal materials for designing biological and chemical sensors based on vibrational spectroscopy. In this paper we review the properties of these glasses and the corresponding design of optical elements for bio-chemical sensing. Amorphous chalcogenides offer a very wide compositional landscape that permit to tune their physical properties to match specific demands for the production of optical devices. This includes tailoring the infrared window over specific ranges of wavelength such as the long-wave infrared region to capture important vibrational signal including the "signature region" of micro-organisms or the bending mode of CO2 molecules. Additionally, compositional engineering enables tuning the viscosity-temperature dependence of the glass melt in order to control the rheological properties that are fundamental to the production of glass elements. Indeed, exquisite control of the viscosity is key to the fabrication process of many optical elements such as fiber drawing, lens molding, surface embossing or reflow of microresonators. Optimal control of these properties then enables the design and fabrication of optimized infrared sensors such as Fiber Evanescent Wave Spectroscopy (FEWS) sensors, Whispering Gallery Modes (WGM) micro-resonator sensors, nanostructured surfaces for integrated optics and surface-enhanced processes, or lens molding for focused collection of infrared signals. Many of these sensor designs can be adapted to collect and monitor the vibrational signal of live microorganisms to study their metabolism in controlled environmental conditions. Further materials
Wearable Wireless Telemetry System for Implantable BioMEMS Sensors
NASA Technical Reports Server (NTRS)
Simons, Rainee N.; Miranda, Felix A.; Wilson, Jeffrey D.; Simons, Renita E.
2008-01-01
Telemetry systems of a type that have been proposed for the monitoring of physiological functions in humans would include the following subsystems: Surgically implanted or ingested units that would comprise combinations of microelectromechanical systems (MEMS)- based sensors [bioMEMS sensors] and passive radio-frequency (RF) readout circuits that would include miniature loop antennas. Compact radio transceiver units integrated into external garments for wirelessly powering and interrogating the implanted or ingested units. The basic principles of operation of these systems are the same as those of the bioMEMS-sensor-unit/external-RFpowering- and-interrogating-unit systems described in "Printed Multi-Turn Loop Antennas for Biotelemetry" (LEW-17879-1) NASA Tech Briefs, Vol. 31, No. 6 (June 2007), page 48, and in the immediately preceding article, "Hand-Held Units for Short-Range Wireless Biotelemetry" (LEW-17483-1). The differences between what is reported here and what was reported in the cited prior articles lie in proposed design features and a proposed mode of operation. In a specific system of the type now proposed, the sensor unit would comprise mainly a capacitive MEMS pressure sensor located in the annular region of a loop antenna (more specifically, a square spiral inductor/ antenna), all fabricated as an integral unit on a high-resistivity silicon chip. The capacitor electrodes, the spiral inductor/antenna, and the conductor lines interconnecting them would all be made of gold. The dimensions of the sensor unit have been estimated to be about 110.4 mm. The external garment-mounted powering/ interrogating unit would include a multi-turn loop antenna and signal-processing circuits. During operation, this external unit would be positioned in proximity to the implanted or ingested unit to provide for near-field, inductive coupling between the loop antennas, which we have as the primary and secondary windings of an electrical transformer.
High-performance flexible strain sensor with bio-inspired crack arrays.
Han, Zhiwu; Liu, Linpeng; Zhang, Junqiu; Han, Qigang; Wang, Kejun; Song, Honglie; Wang, Ze; Jiao, Zhibin; Niu, Shichao; Ren, Luquan
2018-06-12
Biomimetic sensor technology is always superior to existing human technologies. The scorpion, especially the forest scorpion, has a unique ability to detect subtle vibrations, which is attributed to the microcrack-shaped slit sensillum on its legs. Here, the biological sensing mechanism of the typical scorpion (Heterometrus petersii) was intensively studied in order to newly design and significantly improve the flexible strain sensors. Benefiting from the easy-crack property of polystyrene (PS) and using the solvent-induced swelling as well as double template transferring method, regular and controllable microcrack arrays were successfully fabricated on top of polydimethylsiloxane (PDMS). Using this method, any physical damage to PDMS could be effectively avoided. More fortunately, this bio-inspired crack arrays fabricated in this work also had a radial-like pattern similar to the slit sensillum of the scorpion, which was another unexpected imitation. The gauge factor (GF) of the sensor was conservatively evaluated at 5888.89 upon 2% strain and the response time was 297 ms. Afterward, it was demonstrated that the bio-inspired regular microcrack arrays could also significantly enhance the performance of traditional strain sensors, especially in terms of the sensitivity and response time. The practical applications, such as the detection of human motions and surface folding, were also tested in this work, with the results showing significant potential applications in numerous fields. This work changes the traditional waste cracks on some damaged products into valuable things for ultrasensitive mechanical sensors. Moreover, with this manufacturing technique, we could easily realize the simple, low cost and large-scale fabrication of advanced bioinpired sensors.
RF Telemetry System for an Implantable Bio-MEMS Sensor
NASA Technical Reports Server (NTRS)
Simons, Rainee N.; Hall, David G.; Miranda, Felix A.
2004-01-01
In this paper, a novel miniature inductor and a pick-up antenna for contact less powering and RF telemetry from implantable bio-MEMS sensors are presented. The design of the inductor and the pick-up antenna are discussed. In addition, the measured characteristics at the design frequency of 330 MHz have been shown.
Bio-integrated electronics and sensor systems
NASA Astrophysics Data System (ADS)
Yeo, Woon-Hong; Webb, R. Chad; Lee, Woosik; Jung, Sungyoung; Rogers, John A.
2013-05-01
Skin-mounted epidermal electronics, a strategy for bio-integrated electronics, provide an avenue to non-invasive monitoring of clinically relevant physiological signals for healthcare applications. Current conventional systems consist of single-point sensors fastened to the skin with adhesives, and sometimes with conducting gels, which limits their use outside of clinical settings due to loss of adhesion and irritation to the user. In order to facilitate extended use of skin-mounted healthcare sensors without disrupting everyday life, we envision electronic monitoring systems that integrate seamlessly with the skin below the notice of the user. This manuscript reviews recent significant results towards our goal of wearable electronic sensor systems for long-term monitoring of physiological signals. Ultra-thin epidermal electronic systems (EES) are demonstrated for extended use on the skin, in a conformal manner, including during everyday bathing and sleeping activities. We describe the assessment of clinically relevant physiological parameters, such as electrocardiograms (ECG), electromyograms (EMG), electroencephalograms (EEG), temperature, mechanical strain and thermal conductivity, using examples of multifunctional EES devices. Additionally, we demonstrate capability for real life application of EES by monitoring the system functionality, which has no discernible change, during cyclic fatigue testing.
Design and Validation of a 150 MHz HFFQCM Sensor for Bio-Sensing Applications
Fernández, Román; García, Pablo; García, María; Jiménez, Yolanda; Arnau, Antonio
2017-01-01
Acoustic wave resonators have become suitable devices for a broad range of sensing applications due to their sensitivity, low cost, and integration capability, which are all factors that meet the requirements for the resonators to be used as sensing elements for portable point of care (PoC) platforms. In this work, the design, characterization, and validation of a 150 MHz high fundamental frequency quartz crystal microbalance (HFF-QCM) sensor for bio-sensing applications are introduced. Finite element method (FEM) simulations of the proposed design are in good agreement with the electrical characterization of the manufactured resonators. The sensor is also validated for bio-sensing applications. For this purpose, a specific sensor cell was designed and manufactured that addresses the critical requirements associated with this type of sensor and application. Due to the small sensing area and the sensor’s fragility, these requirements include a low-volume flow chamber in the nanoliter range, and a system approach that provides the appropriate pressure control for assuring liquid confinement while maintaining the integrity of the sensor with a good base line stability and easy sensor replacement. The sensor characteristics make it suitable for consideration as the elemental part of a sensor matrix in a multichannel platform for point of care applications. PMID:28885551
Bio-Mimic Optimization Strategies in Wireless Sensor Networks: A Survey
Adnan, Md. Akhtaruzzaman; Razzaque, Mohammd Abdur; Ahmed, Ishtiaque; Isnin, Ismail Fauzi
2014-01-01
For the past 20 years, many authors have focused their investigations on wireless sensor networks. Various issues related to wireless sensor networks such as energy minimization (optimization), compression schemes, self-organizing network algorithms, routing protocols, quality of service management, security, energy harvesting, etc., have been extensively explored. The three most important issues among these are energy efficiency, quality of service and security management. To get the best possible results in one or more of these issues in wireless sensor networks optimization is necessary. Furthermore, in number of applications (e.g., body area sensor networks, vehicular ad hoc networks) these issues might conflict and require a trade-off amongst them. Due to the high energy consumption and data processing requirements, the use of classical algorithms has historically been disregarded. In this context contemporary researchers started using bio-mimetic strategy-based optimization techniques in the field of wireless sensor networks. These techniques are diverse and involve many different optimization algorithms. As far as we know, most existing works tend to focus only on optimization of one specific issue of the three mentioned above. It is high time that these individual efforts are put into perspective and a more holistic view is taken. In this paper we take a step in that direction by presenting a survey of the literature in the area of wireless sensor network optimization concentrating especially on the three most widely used bio-mimetic algorithms, namely, particle swarm optimization, ant colony optimization and genetic algorithm. In addition, to stimulate new research and development interests in this field, open research issues, challenges and future research directions are highlighted. PMID:24368702
Bio-mimic optimization strategies in wireless sensor networks: a survey.
Adnan, Md Akhtaruzzaman; Abdur Razzaque, Mohammd; Ahmed, Ishtiaque; Isnin, Ismail Fauzi
2013-12-24
For the past 20 years, many authors have focused their investigations on wireless sensor networks. Various issues related to wireless sensor networks such as energy minimization (optimization), compression schemes, self-organizing network algorithms, routing protocols, quality of service management, security, energy harvesting, etc., have been extensively explored. The three most important issues among these are energy efficiency, quality of service and security management. To get the best possible results in one or more of these issues in wireless sensor networks optimization is necessary. Furthermore, in number of applications (e.g., body area sensor networks, vehicular ad hoc networks) these issues might conflict and require a trade-off amongst them. Due to the high energy consumption and data processing requirements, the use of classical algorithms has historically been disregarded. In this context contemporary researchers started using bio-mimetic strategy-based optimization techniques in the field of wireless sensor networks. These techniques are diverse and involve many different optimization algorithms. As far as we know, most existing works tend to focus only on optimization of one specific issue of the three mentioned above. It is high time that these individual efforts are put into perspective and a more holistic view is taken. In this paper we take a step in that direction by presenting a survey of the literature in the area of wireless sensor network optimization concentrating especially on the three most widely used bio-mimetic algorithms, namely, particle swarm optimization, ant colony optimization and genetic algorithm. In addition, to stimulate new research and development interests in this field, open research issues, challenges and future research directions are highlighted.
LSI-based amperometric sensor for bio-imaging and multi-point biosensing.
Inoue, Kumi Y; Matsudaira, Masahki; Kubo, Reyushi; Nakano, Masanori; Yoshida, Shinya; Matsuzaki, Sakae; Suda, Atsushi; Kunikata, Ryota; Kimura, Tatsuo; Tsurumi, Ryota; Shioya, Toshihito; Ino, Kosuke; Shiku, Hitoshi; Satoh, Shiro; Esashi, Masayoshi; Matsue, Tomokazu
2012-09-21
We have developed an LSI-based amperometric sensor called "Bio-LSI" with 400 measurement points as a platform for electrochemical bio-imaging and multi-point biosensing. The system is comprised of a 10.4 mm × 10.4 mm CMOS sensor chip with 20 × 20 unit cells, an external circuit box, a control unit for data acquisition, and a DC power box. Each unit cell of the chip contains an operational amplifier with a switched-capacitor type I-V converter for in-pixel signal amplification. We successfully realized a wide dynamic range from ±1 pA to ±100 nA with a well-organized circuit design and operating software. In particular, in-pixel signal amplification and an original program to control the signal read-out contribute to the lower detection limit and wide detection range of Bio-LSI. The spacial resolution is 250 μm and the temporal resolution is 18-125 ms/400 points, which depends on the desired current detection range. The coefficient of variance of the current for 400 points is within 5%. We also demonstrated the real-time imaging of a biological molecule using Bio-LSI. The LSI coated with an Os-HRP film was successfully applied to the monitoring of the changes of hydrogen peroxide concentration in a flow. The Os-HRP-coated LSI was spotted with glucose oxidase and used for bioelectrochemical imaging of the glucose oxidase (GOx)-catalyzed oxidation of glucose. Bio-LSI is a promising platform for a wide range of analytical fields, including diagnostics, environmental measurements and basic biochemistry.
Bio-inspired secure data mules for medical sensor network
NASA Astrophysics Data System (ADS)
Muraleedharan, Rajani; Gao, Weihua; Osadciw, Lisa A.
2010-04-01
Medical sensor network consist of heterogeneous nodes, wireless, mobile and wired with varied functionality. The resources at each sensor require to be exploited minimally while sensitive information is sensed and communicated to its access points using secure data mules. In this paper, we analyze the flat architecture, where different functionality and priority information require varied resources forms a non-deterministic polynomial-time hard problem. Hence, a bio-inspired data mule that helps to obtain dynamic multi-objective solution with minimal resource and secure path is applied. The performance of the proposed approach is based on reduced latency, data delivery rate and resource cost.
Biochemical gas sensor (bio-sniffer) for ultrahigh-sensitive gaseous formaldehyde monitoring.
Kudo, Hiroyuki; Suzuki, Yuki; Gessei, Tomoko; Takahashi, Daishi; Arakawa, Takahiro; Mitsubayashi, Kohji
2010-10-15
An ultrahigh-sensitive fiber-optic biochemical gas sensor (bio-sniffer) for continuous monitoring of indoor formaldehyde was constructed and tested. The bio-sniffer measures gaseous formaldehyde as fluorescence of nicotinamide adenine dinucleotide (NADH), which is the product of formaldehyde dehydrogenase (FALDH) reaction. The bio-sniffer device was constructed by attaching a flow cell with a FALDH immobilized membrane onto a fiber-optic NADH measurement system. The NADH measurement system utilizes an ultraviolet-light emitting diode (UV-LED) with peak emission of 335 nm as an excitation light source. The excitation light was introduced to an optical fiber probe, and fluorescence emission of neighboring NADH, which was produced by applying formaldehyde vapor to the FALDH membrane, was concentrically measured with a photomultiplier tube. Assessment of the bio-sniffer was carried out using a standard gas generator. Response, calibration range and selectivity to other chemical substances were investigated. Circulating phosphate buffer, which contained NAD+, available for continuous monitoring of formaldehyde vapor. The calibration range of the bio-sniffer was 2.5 ppb to 10 ppm, which covers the guideline value of the World Health Organization (80 ppb). High selectivity to other gaseous substances due to specific activity of FALDH was also confirmed. Considering its high sensitivity, a possible application of the bio-sniffer is continuous indoor formaldehyde monitoring to provide healthy residential atmosphere. Copyright © 2010 Elsevier B.V. All rights reserved.
Novel Membrane-Based Electrochemical Sensor for Real-Time Bio-Applications
Alatraktchi, Fatima AlZahra'a; Bakmand, Tanya; Dimaki, Maria; Svendsen, Winnie E.
2014-01-01
This article presents a novel membrane-based sensor for real-time electrochemical investigations of cellular- or tissue cultures. The membrane sensor enables recording of electrical signals from a cell culture without any signal dilution, thus avoiding loss of sensitivity. Moreover, the porosity of the membrane provides optimal culturing conditions similar to existing culturing techniques allowing more efficient nutrient uptake and molecule release. The patterned sensor electrodes were fabricated on a porous membrane by electron-beam evaporation. The electrochemical performance of the membrane electrodes was characterized by cyclic voltammetry and chronoamperometry, and the detection of synthetic dopamine was demonstrated down to a concentration of 3.1 pM. Furthermore, to present the membrane-sensor functionality the dopamine release from cultured PC12 cells was successfully measured. The PC12 cells culturing experiments showed that the membrane-sensor was suitable as a cell culturing substrate for bio-applications. Real-time measurements of dopamine exocytosis in cell cultures were performed, where the transmitter release was recorded at the point of release. The developed membrane-sensor provides a new functionality to the standard culturing methods, enabling sensitive continuous in vitro monitoring and closely mimicking the in vivo conditions. PMID:25421738
Viral vectors for gene modification of plants as chem/bio sensors.
DOE Office of Scientific and Technical Information (OSTI.GOV)
Manginell, Monica; Harper, Jason C.; Arango, Dulce C.
2006-11-01
Chemical or biological sensors that are specific, sensitive, and robust allowing intelligence gathering for verification of nuclear non-proliferation treaty compliance and detouring production of weapons of mass destruction are sorely needed. Although much progress has been made in the area of biosensors, improvements in sensor lifetime, robustness, and device packaging are required before these devices become widely used. Current chemical and biological detection and identification techniques require less-than-covert sample collection followed by transport to a laboratory for analysis. In addition to being expensive and time consuming, results can often be inconclusive due to compromised sample integrity during collection and transport.more » We report here a demonstration of a plant based sensor technology which utilizes mature and seedling plants as chemical sensors. One can envision genetically modifying native plants at a site of interest that can report the presence of specific toxins or chemicals. In this one year project we used a developed inducible expression system to show the feasibility of plant sensors. The vector was designed as a safe, non-infectious vector which could be used to invade, replicate, and introduce foreign genes into mature host plants that then allow the plant to sense chem/bio agents. The genes introduced through the vector included a reporter gene that encodes for green fluorescent protein (GFP) and a gene that encodes for a mammalian receptor that recognizes a chemical agent. Specifically, GFP was induced by the presence of 17-{beta}-Estradiol (estrogen). Detection of fluorescence indicated the presence of the target chemical agent. Since the sensor is a plant, costly device packaging development or manufacturing of the sensor were not required. Additionally, the biological recognition and reporting elements are maintained in a living, natural environment and therefore do not suffer from lifetime disadvantages typical of most
Real-time bio-sensors for enhanced C2ISR operator performance
NASA Astrophysics Data System (ADS)
Miller, James C.
2005-05-01
The objectives of two Air Force Small Business research topics were to develop a real-time, unobtrusive, biological sensing and monitoring technology for evaluating cognitive readiness in command and control environments (i.e., console operators). We sought an individualized status monitoring system for command and control operators and teams. The system was to consist of a collection of bio-sensing technologies and processing and feedback algorithms that could eventually guide the effective incorporation of fatigue-adaptive workload interventions into weapon systems to mitigate episodes of cognitive overload and lapses in operator attention that often result in missed signals and catastrophic failures. Contractors set about determining what electro-physiological and other indicators of compromised operator states are most amenable for unobtrusive monitoring of psychophysiological and warfighter performance data. They proposed multi-sensor platforms of bio-sensing technologies for development. The sensors will be continuously-wearable or off-body and will not require complicated or uncomfortable preparation. A general overview of the proposed approaches and of progress toward the objective is presented.
Bio-optical sensor for brain activity measurement based on whispering gallery modes
NASA Astrophysics Data System (ADS)
Ali, Amir R.; Massoud, Yasmin M.
2017-05-01
In this paper, a high-resolution bio-optical sensor is developed for brain activity measurement. The aim is to develop an optical sensor with enough sensitivity to detect small electric field perturbations caused by neuronal action potential. The sensing element is a polymeric dielectric micro-resonator fabricated in a spherical shape with a few hundred microns in diameter. They are made of optical quality polymers that are soft which make them mechanically compatible with tissue. The sensors are attached to or embedded in optical fibers which serve as input/output conduits for the sensors. Hundreds or even thousands of spheres can be attached to a single fiber to detect and transmit signals at different locations. The high quality factor for the optical resonator makes it significantly used in such bio-medical applications. The sensing phenomenon is based on whispering gallery modes (WGM) shifts of the optical sensor. To mimic the brain signals, the spherical resonator is immersed in a homogeneous electrical field that is created by applying potential difference across two metallic plates. One of the plates has a variable voltage while the volt on the other plate kept fixed. Any small perturbations of the potential difference (voltage) lead to change in the electric field intensity. In turn the sensor morphology will be affected due to the change in the electrostriction force acting on it causing change in its WGM. By tracking these WGM shift on the transmission spectrum, the induced potential difference (voltage change) could be measured. Results of a mathematical model simulation agree well with the preliminary experiments. Also, the results show that the brain activity could be measured using this principle.
Bio-Inspired Asynchronous Pixel Event Tricolor Vision Sensor.
Lenero-Bardallo, Juan Antonio; Bryn, D H; Hafliger, Philipp
2014-06-01
This article investigates the potential of the first ever prototype of a vision sensor that combines tricolor stacked photo diodes with the bio-inspired asynchronous pixel event communication protocol known as Address Event Representation (AER). The stacked photo diodes are implemented in a 22 × 22 pixel array in a standard STM 90 nm CMOS process. Dynamic range is larger than 60 dB and pixels fill factor is 28%. The pixels employ either simple pulse frequency modulation (PFM) or a Time-to-First-Spike (TFS) mode. A heuristic linear combination of the chip's inherent pseudo colors serves to approximate RGB color representation. Furthermore, the sensor outputs can be processed to represent the radiation in the near infrared (NIR) band without employing external filters, and to color-encode direction of motion due to an asymmetry in the update rates of the different diode layers.
Fiber sensor for non-contact estimation of vital bio-signs
NASA Astrophysics Data System (ADS)
Sirkis, Talia; Beiderman, Yevgeny; Agdarov, Sergey; Beiderman, Yafim; Zalevsky, Zeev
2017-05-01
Continuous noninvasive measurement of vital bio-signs, such as cardiopulmonary parameters, is an important tool in evaluation of the patient's physiological condition and health monitoring. On the demand of new enabling technologies, some works have been done in arterial pulse monitoring using optical fiber sensors. In this paper, we introduce a novel device based on single mode in-fibers Mach-Zehnder interferometer (MZI) to detect heartbeat, respiration and pulse wave velocity (PWV). The introduced interferometer is based on a new implanted scheme. It replaces the conventional MZI realized by inserting of discontinuities in the fiber to break the total internal reflection and scatter/collect light. The proposed fiber sensor was successfully incorporated into shirt to produce smart clothing. The measurements obtained from the smart clothing could be obtained in comfortable manner and there is no need to have an initial calibration or a direct contact between the sensor and the skin of the tested individual.
Bio-medical flow sensor. [intrvenous procedures
NASA Technical Reports Server (NTRS)
Winkler, H. E. (Inventor)
1981-01-01
A bio-medical flow sensor including a packageable unit of a bottle, tubing and hypodermic needle which can be pre-sterilized and is disposable. The tubing has spaced apart tubular metal segments. The temperature of the metal segments and fluid flow therein is sensed by thermistors and at a downstream location heat is input by a resistor to the metal segment by a control electronics. The fluids flow and the electrical power required for the resisto to maintain a constant temperature differential between the tubular metal segments is a measurable function of fluid flow through the tubing. The differential temperature measurement is made in a control electronics and also can be used to control a flow control valve or pump on the tubing to maintain a constant flow in the tubing and to shut off the tubing when air is present in the tubing.
Artificial Roughness Encoding with a Bio-inspired MEMS- based Tactile Sensor Array
Oddo, Calogero Maria; Beccai, Lucia; Felder, Martin; Giovacchini, Francesco; Carrozza, Maria Chiara
2009-01-01
A compliant 2×2 tactile sensor array was developed and investigated for roughness encoding. State of the art cross shape 3D MEMS sensors were integrated with polymeric packaging providing in total 16 sensitive elements to external mechanical stimuli in an area of about 20 mm2, similarly to the SA1 innervation density in humans. Experimental analysis of the bio-inspired tactile sensor array was performed by using ridged surfaces, with spatial periods from 2.6 mm to 4.1 mm, which were indented with regulated 1N normal force and stroked at constant sliding velocity from 15 mm/s to 48 mm/s. A repeatable and expected frequency shift of the sensor outputs depending on the applied stimulus and on its scanning velocity was observed between 3.66 Hz and 18.46 Hz with an overall maximum error of 1.7%. The tactile sensor could also perform contact imaging during static stimulus indentation. The experiments demonstrated the suitability of this approach for the design of a roughness encoding tactile sensor for an artificial fingerpad. PMID:22412304
Image sensor system with bio-inspired efficient coding and adaptation.
Okuno, Hirotsugu; Yagi, Tetsuya
2012-08-01
We designed and implemented an image sensor system equipped with three bio-inspired coding and adaptation strategies: logarithmic transform, local average subtraction, and feedback gain control. The system comprises a field-programmable gate array (FPGA), a resistive network, and active pixel sensors (APS), whose light intensity-voltage characteristics are controllable. The system employs multiple time-varying reset voltage signals for APS in order to realize multiple logarithmic intensity-voltage characteristics, which are controlled so that the entropy of the output image is maximized. The system also employs local average subtraction and gain control in order to obtain images with an appropriate contrast. The local average is calculated by the resistive network instantaneously. The designed system was successfully used to obtain appropriate images of objects that were subjected to large changes in illumination.
Lu, Lu
2018-07-01
Electrochemical (bio)sensors have attracted much attention due to their high sensitivity, fast response time, biocompatibility, low cost and easy miniaturization. Specially, ever-growing necessity and interest have given rise to the fast development of electrochemical (bio)sensors for the detection of small biomolecules. They play enormous roles in the life processes with various biological function, such as life signal transmission, genetic expression and metabolism. Moreover, their amount in body can be used as an indicator for diagnosis of many diseases. For example, an abnormal concentration of blood glucose can indicate hyperglycemia or hypoglycemia. Graphene (GR) shows great applications in electrochemical (bio)sensors. Compared with two-dimensional (2D) GR that is inclined to stack together due to the strong π-π interaction, monolithic 3D porous GR has larger specific area, superior mechanical strength, better stability, higher conductivity and electrocatalytic activity. So they attracted more and increasing attention as sensing materials for small biomolecules. This review focuses on the recent advances and strategies in the fabrication methods of 3D porous GR and the development of various electrochemical (bio)sensors based on porous GR and its nanocomposites for the detection of small biomolecules. The challenges and future efforts direction of high-performance electrochemical (bio)sensors based on 3D porous GR for more sensitive analysis of small biomolecules are discussed and proposed. It will give readers an overall understanding of their progress and provide some theoretical guidelines for their future efforts and development. Copyright © 2018 Elsevier B.V. All rights reserved.
State-of-the-Art of (Bio)Chemical Sensor Developments in Analytical Spanish Groups
Plata, María Reyes; Contento, Ana María; Ríos, Angel
2010-01-01
(Bio)chemical sensors are one of the most exciting fields in analytical chemistry today. The development of these analytical devices simplifies and miniaturizes the whole analytical process. Although the initial expectation of the massive incorporation of sensors in routine analytical work has been truncated to some extent, in many other cases analytical methods based on sensor technology have solved important analytical problems. Many research groups are working in this field world-wide, reporting interesting results so far. Modestly, Spanish researchers have contributed to these recent developments. In this review, we summarize the more representative achievements carried out for these groups. They cover a wide variety of sensors, including optical, electrochemical, piezoelectric or electro-mechanical devices, used for laboratory or field analyses. The capabilities to be used in different applied areas are also critically discussed. PMID:22319260
A survey on bio inspired meta heuristic based clustering protocols for wireless sensor networks
NASA Astrophysics Data System (ADS)
Datta, A.; Nandakumar, S.
2017-11-01
Recent studies have shown that utilizing a mobile sink to harvest and carry data from a Wireless Sensor Network (WSN) can improve network operational efficiency as well as maintain uniform energy consumption by the sensor nodes in the network. Due to Sink mobility, the path between two sensor nodes continuously changes and this has a profound effect on the operational longevity of the network and a need arises for a protocol which utilizes minimal resources in maintaining routes between the mobile sink and the sensor nodes. Swarm Intelligence based techniques inspired by the foraging behavior of ants, termites and honey bees can be artificially simulated and utilized to solve real wireless network problems. The author presents a brief survey on various bio inspired swarm intelligence based protocols used in routing data in wireless sensor networks while outlining their general principle and operation.
HIgh-Q Optical Micro-cavity Resonators as High Sensitive Bio-chemical and Ultrasonic Sensors
NASA Astrophysics Data System (ADS)
Ling, Tao
Optical micro-cavity resonators have quickly emerged in the past few years as a new sensing platform in a wide range of applications, such as bio-chemical molecular detection, environmental monitoring, acoustic and electromagnetic waves detection. In this thesis, we will mainly focus on developing high sensitivity silica micro-tube resonator bio-chemical sensors and high sensitivity polymer micro-ring resonator acoustic sensors. In high sensitivity silica micro-tube resonator bio-chemical sensors part: We first demonstrated a prism coupled silica micro-tube bio-chemical sensing platform to overcome the reliability problem in a fiber coupled thin wall silica micro-tube sensing platform. In refractive index sensing experiment, a unique resonance mode with sensitivity around 600nm/refractive index unit (RIU) has been observed. Surface sensing experiments also have been performed in this platform to detect lipid monolayer, lipid bilayer, electrostatic self assemble layer-by-layer as well as the interaction between the lipid bilayer and proteins. Then a theoretical study on various sensing properties on the silica micro-tube based sensing platform has been realized. Furthermore, we have proposed a coupled cavity system to further enhance the device's sensitivity above 1000nm/RIU. In high sensitivity polymer micro-ring resonator acoustic sensors part: We first presented a simplified fabrication process and realized a polymer microring with a Q factor around 6000. The fabricated device has been used to detect acoustic wave with noise equivalent pressure (NEP) around 230Pa over 1-75MHz frequency rang, which is comparable to state-of-art piezoelectric transducer and the device's frequency response also have been characterized to be up to 90MHz. A new fabrication process combined with resist reflow and thermal oxidation process has been used to improve the Q factor up to 10 5 and the device's NEP has been tested to be around 88Pa over 1-75MHz range. Further improving the
NASA Astrophysics Data System (ADS)
Woolard, Dwight L.; Luo, Ying; Gelmont, Boris L.; Globus, Tatiana; Jensen, James O.
2005-05-01
A biological(bio)-molecular inspired electronic architecture is presented that offers the potential for defining nanoscale sensor platforms with enhanced capabilities for sensing terahertz (THz) frequency bio-signatures. This architecture makes strategic use of integrated biological elements to enable communication and high-level function within densely-packed nanoelectronic systems. In particular, this architecture introduces a new paradigm for establishing hybrid Electro-THz-Optical (ETO) communication channels where the THz-frequency spectral characteristics that are uniquely associated with the embedded bio-molecules are utilized directly. Since the functionality of this architecture is built upon the spectral characteristics of bio-molecules, this immediately allows for defining new methods for enhanced sensing of THz bio-signatures. First, this integrated sensor concept greatly facilitates the collection of THz bio-signatures associated with embedded bio-molecules via interactions with the time-dependent signals propagating through the nanoelectronic circuit. Second, it leads to a new Multi-State Spectral Sensing (MS3) approach where bio-signature information can be collected from multiple metastable state conformations. This paper will also introduce a new class of prototype devices that utilize THz-sensitive bio-molecules to achieve molecular-level sensing and functionality. Here, new simulation results are presented for a class of bio-molecular components that exhibit the prescribed type of ETO characteristics required for realizing integrated sensor platforms. Most noteworthy, this research derives THz spectral bio-signatures for organic molecules that are amenable to photo-induced metastable-state conformations and establishes an initial scientific foundation and design blueprint for an enhanced THz bio-signature sensing capability.
Iitani, Kenta; Chien, Po-Jen; Suzuki, Takuma; Toma, Koji; Arakawa, Takahiro; Iwasaki, Yasuhiko; Mitsubayashi, Kohji
2018-02-23
Volatile organic compounds (VOCs) exhaled in breath have huge potential as indicators of diseases and metabolisms. Application of breath analysis for disease screening and metabolism assessment is expected since breath samples can be noninvasively collected and measured. In this research, a highly sensitive and selective biochemical gas sensor (bio-sniffer) for gaseous acetaldehyde (AcH) was developed. In the AcH bio-sniffer, a reverse reaction of alcohol dehydrogenase (ADH) was employed for reducing AcH to ethanol and simultaneously consuming a coenzyme, reduced form of nicotinamide adenine dinucleotide (NADH). The concentration of AcH can be quantified by fluorescence detection of NADH that was consumed by reverse reaction of ADH. The AcH bio-sniffer was composed of an ultraviolet light-emitting diode (UV-LED) as an excitation light source, a photomultiplier tube (PMT) as a fluorescence detector, and an optical fiber probe, and these three components were connected with a bifurcated optical fiber. A gas-sensing region of the fiber probe was developed with a flow-cell and an ADH-immobilized membrane. In the experiment, after optimization of the enzyme reaction conditions, the selectivity and dynamic range of the AcH bio-sniffer were investigated. The AcH bio-sniffer showed a short measurement time (within 2 min) and a broad dynamic range for determination of gaseous AcH, 0.02-10 ppm, which encompassed a typical AcH concentration in exhaled breath (1.2-6.0 ppm). Also, the AcH bio-sniffer exhibited a high selectivity to gaseous AcH based on the specificity of ADH. The sensor outputs were observed only from AcH-contained standard gaseous samples. Finally, the AcH bio-sniffer was applied to measure the concentration of AcH in exhaled breath from healthy subjects after ingestion of alcohol. As a result, a significant difference of AcH concentration between subjects with different aldehyde dehydrogenase type 2 (ALDH2) phenotypes was observed. The AcH bio-sniffer can be
Miniaturized Bio-and Chemical-Sensors for Point-of-Care Monitoring of Chronic Kidney Diseases.
Tricoli, Antonio; Neri, Giovanni
2018-03-22
This review reports the latest achievements in point-of-care (POC) sensor technologies for the monitoring of ammonia, creatinine and urea in patients suffering of chronic kidney diseases (CKDs). Abnormal levels of these nitrogen biomarkers are found in the physiological fluids, such as blood, urine and sweat, of CKD patients. Delocalized at-home monitoring of CKD biomarkers via integration of miniaturized, portable, and low cost chemical- and bio-sensors in POC devices, is an emerging approach to improve patients' health monitoring and life quality. The successful monitoring of CKD biomarkers, performed on the different body fluids by means of sensors having strict requirements in term of size, cost, large-scale production capacity, response time and simple operation procedures for use in POC devices, is reported and discussed.
NASA Technical Reports Server (NTRS)
Simons, Rainee N.; Hall, David G.; Miranda, Felix A.
2004-01-01
The paper describes the operation of a patented wireless RF telemetry system, consisting of a bio-MEMS implantable sensor and an external hand held unit, operating over the frequency range of few hundreds of MHz. A MEMS capacitive pressure sensor integrated with a miniature inductor/antenna together constitute the implantable sensor. Signal processing circuits collocated with a printed loop antenna together form the hand held unit, capable of inductively powering and also receiving the telemetry signals from the sensor. The paper in addition, demonstrates a technique to enhance the quality factor and inductance of the inductor in the presence of a lower ground plane and also presents the radiation characteristics of the loop antenna.
Miniaturized Bio-and Chemical-Sensors for Point-of-Care Monitoring of Chronic Kidney Diseases
Tricoli, Antonio
2018-01-01
This review reports the latest achievements in point-of-care (POC) sensor technologies for the monitoring of ammonia, creatinine and urea in patients suffering of chronic kidney diseases (CKDs). Abnormal levels of these nitrogen biomarkers are found in the physiological fluids, such as blood, urine and sweat, of CKD patients. Delocalized at-home monitoring of CKD biomarkers via integration of miniaturized, portable, and low cost chemical- and bio-sensors in POC devices, is an emerging approach to improve patients’ health monitoring and life quality. The successful monitoring of CKD biomarkers, performed on the different body fluids by means of sensors having strict requirements in term of size, cost, large-scale production capacity, response time and simple operation procedures for use in POC devices, is reported and discussed. PMID:29565315
Hughes, Gareth; Westmacott, Kelly; Honeychurch, Kevin C.; Crew, Adrian; Pemberton, Roy M.; Hart, John P.
2016-01-01
This review describes recent advances in the fabrication of electrochemical (bio)sensors based on screen-printing technology involving carbon materials and their application in biomedical, agri-food and environmental analyses. It will focus on the various strategies employed in the fabrication of screen-printed (bio)sensors, together with their performance characteristics; the application of these devices for the measurement of selected naturally occurring biomolecules, environmental pollutants and toxins will be discussed. PMID:27690118
Wearable Wireless Telemetry System for Implantable Bio-MEMS Sensors
NASA Technical Reports Server (NTRS)
Simons, Rainee N.; Miranda, Felix A.; Wilson, Jeffrey D.; Simons, Renita E.
2006-01-01
In this paper, a telemetry and contact-less powering system consisting of an implantable bio-MEMS sensor with a miniature printed square spiral chip antenna and an external wearable garment with printed loop antenna is investigated. The wearable garment pick-up antenna and the implantable chip antenna are in close proximity to each other and hence couple inductively through their near-fields and behave as the primary and the secondary circuits of a transformer, respectively. The numerical and experimental results are graphically presented, and include the design parameter values as a function of the geometry, the relative RF magnetic near-field intensity as a function of the distance and angle, and the current density on the strip conductors, for the implantable chip antenna.
Multi-Modalities Sensor Science
2015-02-28
enhanced multi-mode sensor science. bio -sensing, cross-discipling, multi-physics, nano-technology sailing He +46-8790 8465 1 Final Report for SOARD Project...spectroscopy, nano-technology, biophotonics and multi-physics modeling to produce adaptable bio -nanostructure enhanced multi-mode sensor science. 1...adaptable bio -nanostructure enhanced multi-mode sensor science. The accomplishments includes 1) A General Method for Designing a Radome to Enhance
Towards a Bio-inspired Security Framework for Mission-Critical Wireless Sensor Networks
NASA Astrophysics Data System (ADS)
Ren, Wei; Song, Jun; Ma, Zhao; Huang, Shiyong
Mission-critical wireless sensor networks (WSNs) have been found in numerous promising applications in civil and military fields. However, the functionality of WSNs extensively relies on its security capability for detecting and defending sophisticated adversaries, such as Sybil, worm hole and mobile adversaries. In this paper, we propose a bio-inspired security framework to provide intelligence-enabled security mechanisms. This scheme is composed of a middleware, multiple agents and mobile agents. The agents monitor the network packets, host activities, make decisions and launch corresponding responses. Middleware performs an infrastructure for the communication between various agents and corresponding mobility. Certain cognitive models and intelligent algorithms such as Layered Reference Model of Brain and Self-Organizing Neural Network with Competitive Learning are explored in the context of sensor networks that have resource constraints. The security framework and implementation are also described in details.
Sensors Based on Bio and Biomimetic Receptors in Medical Diagnostic, Environment, and Food Analysis.
Kozitsina, Alisa N; Svalova, Tatiana S; Malysheva, Natalia N; Okhokhonin, Andrei V; Vidrevich, Marina B; Brainina, Khiena Z
2018-04-01
Analytical chemistry is now developing mainly in two areas: automation and the creation of complexes that allow, on the one hand, for simultaneously analyzing a large number of samples without the participation of an operator, and on the other, the development of portable miniature devices for personalized medicine and the monitoring of a human habitat. The sensor devices, the great majority of which are biosensors and chemical sensors, perform the role of the latter. That last line is considered in the proposed review. Attention is paid to transducers, receptors, techniques of immobilization of the receptor layer on the transducer surface, processes of signal generation and detection, and methods for increasing sensitivity and accuracy. The features of sensors based on synthetic receptors and additional components (aptamers, molecular imprinted polymers, biomimetics) are discussed. Examples of bio- and chemical sensors' application are given. Miniaturization paths, new power supply means, and wearable and printed sensors are described. Progress in this area opens a revolutionary era in the development of methods of on-site and in-situ monitoring, that is, paving the way from the "test-tube to the smartphone".
An analytical model for bio-electronic organic field-effect transistor sensors
NASA Astrophysics Data System (ADS)
Macchia, Eleonora; Giordano, Francesco; Magliulo, Maria; Palazzo, Gerardo; Torsi, Luisa
2013-09-01
A model for the electrical characteristics of Functional-Bio-Interlayer Organic Field-Effect Transistors (FBI-OFETs) electronic sensors is here proposed. Specifically, the output current-voltage characteristics of a streptavidin (SA) embedding FBI-OFET are modeled by means of the analytical equations of an enhancement mode p-channel OFET modified according to an ad hoc designed equivalent circuit that is also independently simulated with pspice. An excellent agreement between the model and the experimental current-voltage output characteristics has been found upon exposure to 5 nM of biotin. A good agreement is also found with the SA OFET parameters graphically extracted from the device transfer I-V curves.
NASA Astrophysics Data System (ADS)
Kang, Donghoon; Kim, Heon-Young; Kim, Dae-Hyun
2014-07-01
The rapid growth of bio-(inspired) sensors has led to an improvement in modern healthcare and human-robot systems in recent years. Higher levels of reliability and better flexibility, essential features of these sensors, are very much required in many application fields (e.g. applications at ultra-high temperatures). Fiber-optic sensors, and fiber Bragg grating (FBG) sensors in particular, are being widely studied as suitable sensors for improved structural health monitoring (SHM) due to their many merits. To enhance the thermal reliability of FBG sensors, thermal sensitivity, generally expressed as αf + ξf and considered a constant, should be investigated more precisely. For this purpose, the governing equation of FBG sensors is modified using differential derivatives between the wavelength shift and the temperature change in this study. Through a thermal test ranging from RT to 900 °C, the thermal sensitivity of FBG sensors is successfully examined and this guarantees thermal reliability of FBG sensors at ultra-high temperatures. In detail, αf + ξf has a non-linear dependence on temperature and varies from 6.0 × 10-6 °C-1 (20 °C) to 10.6 × 10-6 °C-1 (650 °C). Also, FBGs should be carefully used for applications at ultra-high temperatures due to signal disappearance near 900 °C.
C-MEMS for bio-sensing applications
NASA Astrophysics Data System (ADS)
Song, Yin; Agrawal, Richa; Wang, Chunlei
2015-05-01
Developing highly sensitive, selective, and reproducible miniaturized bio-sensing platforms require reliable biointerface which should be compatible with microfabrication techniques. In this study, we have fabricated pyrolyzed carbon arrays with high surface area as a bio-sensing electrode, and developed the surface functionalization methods to increase biomolecules immobilization efficiency and further understand electrochemical phenomena at biointerfaces. The carbon microelectrode arrays with high aspect ratio have been fabricated by carbon microelectromechanical systems (C-MEMS) and nanomaterials such as graphene have been integrated to further increase surface area. To achieve the efficient covalent immobilization of biomolecules, various oxidation and reduction functionalization methods have been investigated. The oxidation treatment in this study includes vacuum ultraviolet, electrochemical activation, UV/Ozone and oxygen RIE. The reduction treatment includes direct amination and diazonium grafting. The developed bio-sensing platform was then applied for several applications, such as: DNA sensor; H2O2 sensor; aptamer sensor and HIV sensor.
Energy harvesting for human wearable and implantable bio-sensors.
Mitcheson, Paul D
2010-01-01
There are clear trade-offs between functionality, battery lifetime and battery volume for wearable and implantable wireless-biosensors which energy harvesting devices may be able to overcome. Reliable energy harvesting has now become a reality for machine condition monitoring and is finding applications in chemical process plants, refineries and water treatment works. However, practical miniature devices that can harvest sufficient energy from the human body to power a wireless bio-sensor are still in their infancy. This paper reviews the options for human energy harvesting in order to determine power availability for harvester-powered body sensor networks. The main competing technologies for energy harvesting from the human body are inertial kinetic energy harvesting devices and thermoelectric devices. These devices are advantageous to some other types as they can be hermetically sealed. In this paper the fundamental limit to the power output of these devices is compared as a function of generator volume when attached to a human whilst walking and running. It is shown that the kinetic energy devices have the highest fundamental power limits in both cases. However, when a comparison is made between the devices using device effectivenesses figures from previously demonstrated prototypes presented in the literature, the thermal device is competitive with the kinetic energy harvesting device when the subject is running and achieves the highest power density when the subject is walking.
Bio-inspired group modeling and analysis for intruder detection in mobile sensor/robotic networks.
Fu, Bo; Xiao, Yang; Liang, Xiannuan; Philip Chen, C L
2015-01-01
Although previous bio-inspired models have concentrated on invertebrates (such as ants), mammals such as primates with higher cognitive function are valuable for modeling the increasingly complex problems in engineering. Understanding primates' social and communication systems, and applying what is learned from them to engineering domains is likely to inspire solutions to a number of problems. This paper presents a novel bio-inspired approach to determine group size by researching and simulating primate society. Group size does matter for both primate society and digital entities. It is difficult to determine how to group mobile sensors/robots that patrol in a large area when many factors are considered such as patrol efficiency, wireless interference, coverage, inter/intragroup communications, etc. This paper presents a simulation-based theoretical study on patrolling strategies for robot groups with the comparison of large and small groups through simulations and theoretical results.
Xian, Zhiwen; Hu, Xiaoping; Lian, Junxiang; Zhang, Lilian; Cao, Juliang; Wang, Yujie; Ma, Tao
2014-09-15
Navigation plays a vital role in our daily life. As traditional and commonly used navigation technologies, Inertial Navigation System (INS) and Global Navigation Satellite System (GNSS) can provide accurate location information, but suffer from the accumulative error of inertial sensors and cannot be used in a satellite denied environment. The remarkable navigation ability of animals shows that the pattern of the polarization sky can be used for navigation. A bio-inspired POLarization Navigation Sensor (POLNS) is constructed to detect the polarization of skylight. Contrary to the previous approach, we utilize all the outputs of POLNS to compute input polarization angle, based on Least Squares, which provides optimal angle estimation. In addition, a new sensor calibration algorithm is presented, in which the installation angle errors and sensor biases are taken into consideration. Derivation and implementation of our calibration algorithm are discussed in detail. To evaluate the performance of our algorithms, simulation and real data test are done to compare our algorithms with several exiting algorithms. Comparison results indicate that our algorithms are superior to the others and are more feasible and effective in practice.
Bio-inspired approach for intelligent unattended ground sensors
NASA Astrophysics Data System (ADS)
Hueber, Nicolas; Raymond, Pierre; Hennequin, Christophe; Pichler, Alexander; Perrot, Maxime; Voisin, Philippe; Moeglin, Jean-Pierre
2015-05-01
Improving the surveillance capacity over wide zones requires a set of smart battery-powered Unattended Ground Sensors capable of issuing an alarm to a decision-making center. Only high-level information has to be sent when a relevant suspicious situation occurs. In this paper we propose an innovative bio-inspired approach that mimics the human bi-modal vision mechanism and the parallel processing ability of the human brain. The designed prototype exploits two levels of analysis: a low-level panoramic motion analysis, the peripheral vision, and a high-level event-focused analysis, the foveal vision. By tracking moving objects and fusing multiple criteria (size, speed, trajectory, etc.), the peripheral vision module acts as a fast relevant event detector. The foveal vision module focuses on the detected events to extract more detailed features (texture, color, shape, etc.) in order to improve the recognition efficiency. The implemented recognition core is able to acquire human knowledge and to classify in real-time a huge amount of heterogeneous data thanks to its natively parallel hardware structure. This UGS prototype validates our system approach under laboratory tests. The peripheral analysis module demonstrates a low false alarm rate whereas the foveal vision correctly focuses on the detected events. A parallel FPGA implementation of the recognition core succeeds in fulfilling the embedded application requirements. These results are paving the way of future reconfigurable virtual field agents. By locally processing the data and sending only high-level information, their energy requirements and electromagnetic signature are optimized. Moreover, the embedded Artificial Intelligence core enables these bio-inspired systems to recognize and learn new significant events. By duplicating human expertise in potentially hazardous places, our miniature visual event detector will allow early warning and contribute to better human decision making.
Engineering carbon nanomaterials for future applications: energy and bio-sensor
NASA Astrophysics Data System (ADS)
Das, Santanu; Lahiri, Indranil; Kang, Chiwon; Choi, Wonbong
2011-06-01
This paper presents our recent results on carbon nanomaterials for applications in energy storage and bio-sensor. More specifically: (i) A novel binder-free carbon nanotubes (CNTs) structure as anode in Li-ion batteries. The interfacecontrolled CNT structure, synthesized through a two-step chemical vapor deposition (CVD) and directly grown on copper current collector, showed very high specific capacity - almost three times as that of graphite, excellent rate capability. (ii) A large scale graphene film was grown on Cu foil by thermal chemical vapor deposition and transferred to various substrates including PET, glass and silicon by using hot press lamination and etching process. The graphene/PET film shows high quality, flexible transparent conductive structure with unique electrical-mechanical properties; ~88.80 % light transmittance and ~ 100 Ω/sq sheet resistance. We demonstrate application of graphene/PET film as flexible and transparent electrode for field emission displays. (iii) Application of individual carbon nanotube as nanoelectrode for high sensitivity electrochemical sensor and device miniaturization. An individual CNT is split into a pair of nanoelectrodes with a gap between them. Single molecular-level detection of DNA hybridization was studied. Hybridization of the probe with its complementary strand results in an appreciable change in the electrical output signal.
Integrated bio-fluorescence sensor.
Thrush, Evan; Levi, Ofer; Ha, Wonill; Wang, Ke; Smith, Stephen J; Harris, James S
2003-09-26
Due to the recent explosion in optoelectronics for telecommunication applications, novel optoelectronic sensing structures can now be realized. In this work, we explore the integration of optoelectronic components towards miniature and portable fluorescence sensors. The integration of these micro-fabricated sensors with microfluidics and capillary networks may reduce the cost and complexity of current research instruments and open up a world of new applications in portable biological analysis systems. A novel optoelectronic design that capitalizes on current vertical-cavity surface-emitting laser (VCSEL) technology is explored. Specifically, VCSELs, optical emission filters and PIN photodetectors are fabricated as part of a monolithically integrated near-infrared fluorescence detection system. High-performance lasers and photodetectors have been characterized and integrated to form a complete sensor. Experimental results show that sensor sensitivity is limited by laser background. The laser background is caused by spontaneous emission emitted from the side of the VCSEL excitation source. Laser background will limit sensitivity in most integrated sensing designs due to locating excitation sources and photodetectors in such close proximity, and methods are proposed to reduce the laser background in such designs so that practical fluorescent detection limits can be achieved.
Xian, Zhiwen; Hu, Xiaoping; Lian, Junxiang; Zhang, Lilian; Cao, Juliang; Wang, Yujie; Ma, Tao
2014-01-01
Navigation plays a vital role in our daily life. As traditional and commonly used navigation technologies, Inertial Navigation System (INS) and Global Navigation Satellite System (GNSS) can provide accurate location information, but suffer from the accumulative error of inertial sensors and cannot be used in a satellite denied environment. The remarkable navigation ability of animals shows that the pattern of the polarization sky can be used for navigation. A bio-inspired POLarization Navigation Sensor (POLNS) is constructed to detect the polarization of skylight. Contrary to the previous approach, we utilize all the outputs of POLNS to compute input polarization angle, based on Least Squares, which provides optimal angle estimation. In addition, a new sensor calibration algorithm is presented, in which the installation angle errors and sensor biases are taken into consideration. Derivation and implementation of our calibration algorithm are discussed in detail. To evaluate the performance of our algorithms, simulation and real data test are done to compare our algorithms with several exiting algorithms. Comparison results indicate that our algorithms are superior to the others and are more feasible and effective in practice. PMID:25225872
Performance of a novel wafer scale CMOS active pixel sensor for bio-medical imaging.
Esposito, M; Anaxagoras, T; Konstantinidis, A C; Zheng, Y; Speller, R D; Evans, P M; Allinson, N M; Wells, K
2014-07-07
Recently CMOS active pixels sensors (APSs) have become a valuable alternative to amorphous silicon and selenium flat panel imagers (FPIs) in bio-medical imaging applications. CMOS APSs can now be scaled up to the standard 20 cm diameter wafer size by means of a reticle stitching block process. However, despite wafer scale CMOS APS being monolithic, sources of non-uniformity of response and regional variations can persist representing a significant challenge for wafer scale sensor response. Non-uniformity of stitched sensors can arise from a number of factors related to the manufacturing process, including variation of amplification, variation between readout components, wafer defects and process variations across the wafer due to manufacturing processes. This paper reports on an investigation into the spatial non-uniformity and regional variations of a wafer scale stitched CMOS APS. For the first time a per-pixel analysis of the electro-optical performance of a wafer CMOS APS is presented, to address inhomogeneity issues arising from the stitching techniques used to manufacture wafer scale sensors. A complete model of the signal generation in the pixel array has been provided and proved capable of accounting for noise and gain variations across the pixel array. This novel analysis leads to readout noise and conversion gain being evaluated at pixel level, stitching block level and in regions of interest, resulting in a coefficient of variation ⩽1.9%. The uniformity of the image quality performance has been further investigated in a typical x-ray application, i.e. mammography, showing a uniformity in terms of CNR among the highest when compared with mammography detectors commonly used in clinical practice. Finally, in order to compare the detection capability of this novel APS with the technology currently used (i.e. FPIs), theoretical evaluation of the detection quantum efficiency (DQE) at zero-frequency has been performed, resulting in a higher DQE for this
NASA Astrophysics Data System (ADS)
Ghosh, Sujoy Kumar; Mandal, Dipankar
2017-03-01
A human interactive self-powered wearable sensor is designed using waste by-product prawn shells. The structural origin of intrinsic piezoelectric characteristics of bio-assembled chitin nanofibers has been investigated. It allows the prawn shell to make a tactile sensor that performs also as a highly durable mechanical energy harvester/nanogenerator. The feasibility and fundamental physics of self-powered consumer electronics even from human perception is highlighted by prawn shells made nanogenerator (PSNG). High fidelity and non-invasive monitoring of vital signs, such as radial artery pulse wave and coughing actions, may lead to the potential use of PSNG for early intervention. It is presumed that PSNG has enormous future aspects in real-time as well as remote health care assessment.
Fiber-Optic Chemical Sensors and Fiber-Optic Bio-Sensors
Pospíšilová, Marie; Kuncová, Gabriela; Trögl, Josef
2015-01-01
This review summarizes principles and current stage of development of fiber-optic chemical sensors (FOCS) and biosensors (FOBS). Fiber optic sensor (FOS) systems use the ability of optical fibers (OF) to guide the light in the spectral range from ultraviolet (UV) (180 nm) up to middle infrared (IR) (10 µm) and modulation of guided light by the parameters of the surrounding environment of the OF core. The introduction of OF in the sensor systems has brought advantages such as measurement in flammable and explosive environments, immunity to electrical noises, miniaturization, geometrical flexibility, measurement of small sample volumes, remote sensing in inaccessible sites or harsh environments and multi-sensing. The review comprises briefly the theory of OF elaborated for sensors, techniques of fabrications and analytical results reached with fiber-optic chemical and biological sensors. PMID:26437407
Fiber-Optic Chemical Sensors and Fiber-Optic Bio-Sensors.
Pospíšilová, Marie; Kuncová, Gabriela; Trögl, Josef
2015-09-30
This review summarizes principles and current stage of development of fiber-optic chemical sensors (FOCS) and biosensors (FOBS). Fiber optic sensor (FOS) systems use the ability of optical fibers (OF) to guide the light in the spectral range from ultraviolet (UV) (180 nm) up to middle infrared (IR) (10 μm) and modulation of guided light by the parameters of the surrounding environment of the OF core. The introduction of OF in the sensor systems has brought advantages such as measurement in flammable and explosive environments, immunity to electrical noises, miniaturization, geometrical flexibility, measurement of small sample volumes, remote sensing in inaccessible sites or harsh environments and multi-sensing. The review comprises briefly the theory of OF elaborated for sensors, techniques of fabrications and analytical results reached with fiber-optic chemical and biological sensors.
Seo, Dongmin; Paek, Sung-Ho; Oh, Sangwoo; Seo, Sungkyu; Paek, Se-Hwan
2016-01-01
The incidence of diabetes is continually increasing, and by 2030, it is expected to have increased by 69% and 20% in underdeveloped and developed countries, respectively. Therefore, glucose sensors are likely to remain in high demand in medical device markets. For the current study, we developed a needle-type bio-layer interference (BLI) sensor that can continuously monitor glucose levels. Using dialysis procedures, we were able to obtain hypoglycemic samples from commercial human serum. These dialysis-derived samples, alongside samples of normal human serum were used to evaluate the utility of the sensor for the detection of the clinical interest range of glucose concentrations (70–200 mg/dL), revealing high system performance for a wide glycemic state range (45–500 mg/dL). Reversibility and reproducibility were also tested over a range of time spans. Combined with existing BLI system technology, this sensor holds great promise for use as a wearable online continuous glucose monitoring system for patients in a hospital setting. PMID:27669267
Seo, Dongmin; Paek, Sung-Ho; Oh, Sangwoo; Seo, Sungkyu; Paek, Se-Hwan
2016-09-24
The incidence of diabetes is continually increasing, and by 2030, it is expected to have increased by 69% and 20% in underdeveloped and developed countries, respectively. Therefore, glucose sensors are likely to remain in high demand in medical device markets. For the current study, we developed a needle-type bio-layer interference (BLI) sensor that can continuously monitor glucose levels. Using dialysis procedures, we were able to obtain hypoglycemic samples from commercial human serum. These dialysis-derived samples, alongside samples of normal human serum were used to evaluate the utility of the sensor for the detection of the clinical interest range of glucose concentrations (70-200 mg/dL), revealing high system performance for a wide glycemic state range (45-500 mg/dL). Reversibility and reproducibility were also tested over a range of time spans. Combined with existing BLI system technology, this sensor holds great promise for use as a wearable online continuous glucose monitoring system for patients in a hospital setting.
Bio-Inspired Micromechanical Directional Acoustic Sensor
NASA Astrophysics Data System (ADS)
Swan, William; Alves, Fabio; Karunasiri, Gamani
Conventional directional sound sensors employ an array of spatially separated microphones and the direction is determined using arrival times and amplitudes. In nature, insects such as the Ormia ochracea fly can determine the direction of sound using a hearing organ much smaller than the wavelength of sound it detects. The fly's eardrums are mechanically coupled, only separated by about 1 mm, and have remarkable directional sensitivity. A micromechanical sensor based on the fly's hearing system was designed and fabricated on a silicon on insulator (SOI) substrate using MEMS technology. The sensor consists of two 1 mm2 wings connected using a bridge and to the substrate using two torsional legs. The dimensions of the sensor and material stiffness determine the frequency response of the sensor. The vibration of the wings in response to incident sound at the bending resonance was measured using a laser vibrometer and found to be about 1 μm/Pa. The electronic response of the sensor to sound was measured using integrated comb finger capacitors and found to be about 25 V/Pa. The fabricated sensors showed good directional sensitivity. In this talk, the design, fabrication and characteristics of the directional sound sensor will be described. Supported by ONR and TDSI.
NASA Technical Reports Server (NTRS)
Nessel, James A.; Simons, Rainee N.; Miranda, Felix A.
2007-01-01
The near field radiation characteristics of implantable Square Spiral Chip Inductor Antennas (SSCIA) for Bio-Sensors have been measured. Our results indicate that the measured near field relative signal strength of these antennas agrees with simulated results and confirm that in the near field region the radiation field is fairly uniform in all directions. The effects of parameters such as ground-plane, number of turns and microstrip-gap width on the performance of the SSCIA are presented. Furthermore, the SSCIA antenna with serrated ground plane produce a broad radiation pattern, with a relative signal strength detectable at distances within the range of operation of hand-held devices for self-diagnosis.
Chem/bio sensing with non-classical light and integrated photonics.
Haas, J; Schwartz, M; Rengstl, U; Jetter, M; Michler, P; Mizaikoff, B
2018-01-29
Modern quantum technology currently experiences extensive advances in applicability in communications, cryptography, computing, metrology and lithography. Harnessing this technology platform for chem/bio sensing scenarios is an appealing opportunity enabling ultra-sensitive detection schemes. This is further facilliated by the progress in fabrication, miniaturization and integration of visible and infrared quantum photonics. Especially, the combination of efficient single-photon sources together with waveguiding/sensing structures, serving as active optical transducer, as well as advanced detector materials is promising integrated quantum photonic chem/bio sensors. Besides the intrinsic molecular selectivity and non-destructive character of visible and infrared light based sensing schemes, chem/bio sensors taking advantage of non-classical light sources promise sensitivities beyond the standard quantum limit. In the present review, recent achievements towards on-chip chem/bio quantum photonic sensing platforms based on N00N states are discussed along with appropriate recognition chemistries, facilitating the detection of relevant (bio)analytes at ultra-trace concentration levels. After evaluating recent developments in this field, a perspective for a potentially promising sensor testbed is discussed for reaching integrated quantum sensing with two fiber-coupled GaAs chips together with semiconductor quantum dots serving as single-photon sources.
Optical bio-sniffer for methyl mercaptan in halitosis.
Mitsubayashi, Kohji; Minamide, Takeshi; Otsuka, Kimio; Kudo, Hiroyuki; Saito, Hirokazu
2006-07-28
An optical bio-sniffer for methyl mercaptan (MM) one of major odorous chemicals in halitosis (bad breath) was constructed by immobilizing monoamine oxidase type A (MAO-A) onto a tip of a fiber optic oxygen sensor (od: 1.59 mm) with an oxygen sensitive ruthenium organic complex (excitation: 470 nm, fluorescent: 600 nm). A flow cell for circulating buffer solution was applied to rinse and clean the tip of the device like nasal mucosa. In order to amplify the bio-sniffer output, a substrate regeneration cycle caused by coupling MAO-A with l-ascorbic acid (AsA) as reducing reaction with reagent system was applied to the sensor system. After evaluating the sensor characteristics using a gas flow measurement system with a gas generator, the optical bio-sniffer was applied to expired gases from healthy male volunteers for halitosis analysis as a physiological application. The optical bio-sniffer was applied to detect the oxygen consumption induced by MAO-A enzymatic reaction (and AsA chemical reduction) with gaseous MM application. The bio-sniffer was calibrated against MM vapor from 8.7 to 11500 ppb with correlation coefficient of 0.977, including a MM threshold (200 ppb) of pathologic halitosis and the human sense of smell level 3.5 (10.0 ppb), with good gas-selectivity based on the MAO-A substrate specificity. As the result of the physiological application, the optical bio-sniffer could successfully monitor the MM level change in breath samples during daytime, which is consistent with the previously reported results.
NASA Technical Reports Server (NTRS)
Ghoshal, Anindya; Prosser, William H.; Kirikera, Goutham; Schulz, Mark J.; Hughes, Derke J.; Orisamolu, Wally
2003-01-01
This paper discusses the modeling of acoustic emissions in plate structures and their sensing by embedded or surface bonded piezoelectric sensor arrays. Three different modeling efforts for acoustic emission (AE) wave generation and propagation are discussed briefly along with their advantages and disadvantages. Continuous sensors placed at right angles on a plate are being discussed as a new approach to measure and locate the source of acoustic waves. Evolutionary novel signal processing algorithms and bio-inspired distributed sensor array systems are used on large structures and integrated aerospace vehicles for AE source localization and preliminary results are presented. These systems allow for a great reduction in the amount of data that needs to be processed and also reduce the chances of false alarms from ambient noises. It is envisioned that these biomimetic sensor arrays and signal processing techniques will be useful for both wireless and wired sensor arrays for real time health monitoring of large integrated aerospace vehicles and earth fixed civil structures. The sensor array architectures can also be used with other types of sensors and for other applications.
Fabrication of plasmonic nanopore by using electron beam irradiation for optical bio-sensor
NASA Astrophysics Data System (ADS)
Choi, Seong Soo; Park, Myoung Jin; Han, Chul Hee; Oh, Seh Joong; Park, Nam Kyou; Park, Doo Jae; Choi, Soo Bong; Kim, Yong-Sang
2017-05-01
The Au nano-hole surrounded by the periodic nano-patterns would provide the enhanced optical intensity. Hence, the nano-hole surrounded with periodic groove patterns can be utilized as single molecule nanobio optical sensor device. In this report, the nano-hole on the electron beam induced membrane surrounded by periodic groove patterns were fabricated by focused ion beam technique (FIB), field emission scanning electron microscopy (FESEM), and transmission electron microscopy (TEM). Initially, the Au films with three different thickness of 40 nm, 60 nm, and 200 nm were deposited on the SiN film by using an electron beam sputter-deposition technique, followed by removal of the supporting SiN film. The nanopore was formed on the electron beam induced membrane under the FESEM electron beam irradiation. Nanopore formation inside the Au aperture was controlled down to a few nanometer, by electron beam irradiations. The optical intensities from the biomolecules on the surfaces including Au coated pyramid with periodic groove patterns were investigated via surface enhanced Raman spectroscopy (SERS). The fabricated nanopore surrounded by periodic patterns can be utilized as a next generation single molecule bio optical sensor.
Advanced Nanoporous Materials for Micro-Gravimetric Sensing to Trace-Level Bio/Chemical Molecules
Xu, Pengcheng; Li, Xinxin; Yu, Haitao; Xu, Tiegang
2014-01-01
Functionalized nanoporous materials have been developed recently as bio/chemical sensing materials. Due to the huge specific surface of the nano-materials for molecular adsorption, high hopes have been placed on gravimetric detection with micro/nano resonant cantilevers for ultra-sensitive sensing of low-concentration bio/chemical substances. In order to enhance selectivity of the gravimetric resonant sensors to the target molecules, it is crucial to modify specific groups onto the pore-surface of the nano-materials. By loading the nanoporous sensing material onto the desired region of the mass-type transducers like resonant cantilevers, the micro-gravimetric bio/chemical sensors can be formed. Recently, such micro-gravimetric bio/chemical sensors have been successfully applied for rapid or on-the-spot detection of various bio/chemical molecules at the trace-concentration level. The applicable nanoporous sensing materials include mesoporous silica, zeolite, nanoporous graphene oxide (GO) and so on. This review article focuses on the recent achievements in design, preparation, functionalization and characterization of advanced nanoporous sensing materials for micro-gravimetric bio/chemical sensing. PMID:25313499
A High Sensitivity Bio Photosensor for Detecting a Luciferase Bioluminescence
NASA Astrophysics Data System (ADS)
Kameda, Seiji; Moriyama, Yusuke; Noda, Kenichi; Iwata, Atsushi
A high sensitivity CMOS bio photosensor applicable to a bioluminescent assay was developed with a 0.18µm CMOS image sensor (CIS) process. The bio photosensor consisting of a photosensor and a PWM 20bit A/D converter achieved high sensitivity for detecting a extremely low bioluminescence due to a large photodiode area, a long exposure time and the other noise reduction techniques. The bio photosensor chip has a 2×4 sensor array on a 2.45×2.45mm2 die. Experimental results with the bioluminescence showed the chip can detect below 10-5lux luminescence at room temperature and the power consumption is 32µW.
2014-10-01
applications of present nano-/ bio -technology include advanced health and fitness monitoring, high-resolution imaging, new environmental sensor platforms...others areas where nano-/ bio -technology development is needed: • Sensors : Diagnostic and detection kits (gene-chips, protein-chips, lab-on-chips, etc...studies on chemo- bio nano- sensors , ultra-sensitive biochips (“lab-on-a-chip” and “cells-on-chips” devices) have been prepared for routine medical
Mousty, Christine; Leroux, Fabrice
2012-11-01
From an exhaustive overview based on applicative academic literature and patent domain, the relevance of Layered Double Hydroxide (LDHs) as electrode materials for electrochemical detection of organic molecules having environmental or health impact and energy storage is evaluated. Specifically the focus is driven on their application as supercapacitor, alkaline or lithium battery and (bio)-sensor. Inherent to the high versatility of their chemical composition, charge density, anion exchange capability, LDH-based materials are extensively studied and their performances for such applications are reported. Indeed the analytical characteristics (sensitivity and detection limit) of LDH-based electrodes are scrutinized, and their specific capacity or capacitance as electrode battery or supercapacitor materials, are detailed.
Kobayashi, Atsuki; Ikeda, Kei; Ogawa, Yudai; Kai, Hiroyuki; Nishizawa, Matsuhiko; Nakazato, Kazuo; Niitsu, Kiichi
2017-12-01
In this paper, we present a self-powered bio-sensing system with the capability of proximity inductive-coupling communication for supply sensing and temperature monitoring. The proposed bio-sensing system includes a biofuel cell as a power source and a sensing frontend that is associated with the CMOS integrated supply-sensing sensor. The sensor consists of a digital-based gate leakage timer, a supply-insensitive time-domain temperature sensor, and a current-driven inductive-coupling transmitter and achieves low-voltage operation. The timer converts the output voltage from a biofuel cell to frequency. The temperature sensor provides a pulse width modulation (PWM) output that is not dependent on the supply voltage, and the associated inductive-coupling transmitter enables proximity communication. A test chip was fabricated in 65 nm CMOS technology and consumed 53 μW with a supply voltage of 190 mV. The low-voltage-friendly design satisfied the performance targets of each integrated sensor without any trimming. The chips allowed us to successfully demonstrate proximity communication with an asynchronous receiver, and the measurement results show the potential for self-powered operation using biofuel cells. The analysis and experimental verification of the system confirmed their robustness.
Arduini, Fabiana; Cinti, Stefano; Scognamiglio, Viviana; Moscone, Danila; Palleschi, Giuseppe
2017-03-22
Through the years, scientists have developed cutting-edge technologies to make (bio)sensors more convenient for environmental analytical purposes. Technological advancements in the fields of material science, rational design, microfluidics, and sensor printing, have radically shaped biosensor technology, which is even more evident in the continuous development of sensing systems for the monitoring of hazardous chemicals. These efforts will be crucial in solving some of the problems constraining biosensors to reach real environmental applications, such as continuous analyses in field by means of multi-analyte portable devices. This review (with 203 refs.) covers the progress between 2010 and 2015 in the field of technologies enabling biosensor applications in environmental analysis, including i) printing technology, ii) nanomaterial technology, iii) nanomotors, iv) biomimetic design, and (v) microfluidics. Next section describes futuristic cutting-edge technologies that are gaining momentum in recent years, which furnish highly innovative aspects to biosensing devices. Copyright © 2016 Elsevier B.V. All rights reserved.
Dry electrode bio-potential recordings.
Gargiulo, Gaetano; Bifulco, Paolo; McEwan, Alistair; Nasehi Tehrani, Joubin; Calvo, Rafael A; Romano, Maria; Ruffo, Mariano; Shephard, Richard; Cesarelli, Mario; Jin, Craig; Mohamed, Armin; van Schaik, André
2010-01-01
As wireless bio-medical long term monitoring moves towards personal monitoring it demands very high input impedance systems capable to extend the reading of bio-signal during the daily activities offering a kind of "stress free", convenient connection, with no need for skin preparation. In particular we highlight the development and broad applications of our own circuits for wearable bio-potential sensor systems enabled by the use of an FET based amplifier circuit with sufficiently high impedance to allow the use of passive dry electrodes which overcome the significant barrier of gel based contacts. In this paper we present the ability of dry electrodes in long term monitoring of ECG, EEG and fetal ECG.
Design and Characterization of a Novel Bio-inspired Hair Flow Sensor Based on Resonant Sensing
NASA Astrophysics Data System (ADS)
Guo, X.; Yang, B.; Wang, Q. H.; Lu, C. F.; Hu, D.
2018-03-01
Flow sensors inspired by the natural hair sensing mechanism have great prospect in the research of micro-autonomous system and technology (MAST) for the three-dimensional structure characteristics with high spatial and quality utilization. A novel bio-inspired hair flow sensor (BHFS) based on resonant sensing with a unique asymmetric design is presented in this paper. A hair transducer and a signal detector which is constituted of a two-stage micro-leverage mechanism and two symmetrical resonators (double ended tuning fork, DETF) are adopted to realize the high sensitivity to air flow. The sensitivity of the proposed BHFS is improved significantly than the published ones due to the high sensitivity of resonators and the higher amplification factor possessed by the two-stage micro-leverage mechanism. The standard deep dry silicon on glass (DDSOG) process is chosen to fabricate the proposed BHFS. The experiment result demonstrates that the fabricated BHFS has a mechanical sensitivity of 5.26 Hz/(m/s)2 at a resonant frequency of 22 kHz with the hair height of 6 mm.
NASA Astrophysics Data System (ADS)
Antarnusa, G.; Elda Swastika, P.; Suharyadi, E.
2018-04-01
A Wheatstone bridge-giant magnetoresistance (GMR) sensor was successfully developed for a potential biomaterial detection. In order to achieve this, a giant magnetoresistive [Co(1.5nm/Cu(1.0nm)]20 multilayer structures have been fabricated by DC magnetron sputtering method, showing a magnetoresistance (MR) of 2.7%. The X-Ray diffraction (XRD) patterns showed that Co/Cu film multilayer has a high degree of crystallinity with a single peak corresponding to face-centered cubic (111) structure at 2θ = 44.1°. Co/Cu multilayers exhibit a soft magnetic behavior with the saturation magnetization (Ms) of 1489 emu/cc and the coercivity (Hc) of 11.2 Oe. The magnetite Fe3O4 nanoparticles used as a bimolecular labels (nanotags) were synthesized via co-precipitation method, exhibiting a soft magnetic behavior with Ms of 77.16 emu/g and Hc of 49 Oe. XRD patterns and transmission electron microscopy (TEM) images showed that Fe3O4 was well crystallized and it grew in their inverse spinel structure with an average size of around 10 nm. The GMR sensor design was used to detect a biomolecules of streptavidin magnetic particles with concentration 10, 20, 30, and 40 μl/ml and α-amylase enzyme with consentration 10, 20, 30, and 40 μl/ml captured using polyethylene glycol (PEG)/Fe3O4 nanoparticles. Various applied magnetic fields of 0-650 Gauss have been performed using electromagnetic with the various currents of 0-5 A. Here, the final value of the output voltage signals for the streptavidin magnetic particles concentration is 1.2 mV (10 μl/ml). The output voltage changes with the increase of concentration. It was reported that the output voltage signal of the Wheatstone bridge exhibits log-linear function in real time measurement of the concentration of streptavidin magnetic particles and α-amylase enzyme respectively, making the sensor suitable for use as a biomolecule concentration detector. Thus, the combination of Co/Cu multilayer, Wheatstone bridge, magnetite and PEG polymer
Baranwal, Anupriya; Kumar, Ashutosh; Priyadharshini, A; Oggu, Gopi Suresh; Bhatnagar, Ira; Srivastava, Ananya; Chandra, Pranjal
2018-04-15
Biopolymers have been serving the mankind in various ways since long. Over the last few years, these polymers have found great demand in various domains which includes bio medicine, tissue engineering, bio sensor fabrications etc. because of their excellent bio compatibility. In this context, chitosan has found global attention due to its environmentally benign nature, biocompatibility, biodegradability, and ease of availability. In last one decade or so, extensive research in active biomaterials, like chitosan has led to the development of novel delivery systems for drugs, genes, and biomolecules; and regenerative medicine. Additionally, chitosan has also witnessed its usage in functionalization of biocompatible materials, nanoparticle (NP) synthesis, and immobilization of various bio-recognition elements (BREs) to form active bio-surfaces with great ease. Keeping these aspects in mind, we have written a comprehensive review which aims to acquaint its readers with the exceptional properties of chitosan and its usage in the domain of biomedicine, tissue engineering, and biosensor fabrication. Herein, we have briefly explained various aspects of direct utilization of chitosan and then presented vivid strategies towards formulation of chitosan based nanocomposites for biomedicine, tissue engineering, and biosensing applications. Copyright © 2018 Elsevier B.V. All rights reserved.
Bio-Benchmarking of Electronic Nose Sensors
Berna, Amalia Z.; Anderson, Alisha R.; Trowell, Stephen C.
2009-01-01
Background Electronic noses, E-Noses, are instruments designed to reproduce the performance of animal noses or antennae but generally they cannot match the discriminating power of the biological original and have, therefore, been of limited utility. The manner in which odorant space is sampled is a critical factor in the performance of all noses but so far it has been described in detail only for the fly antenna. Methodology Here we describe how a set of metal oxide (MOx) E-Nose sensors, which is the most commonly used type, samples odorant space and compare it with what is known about fly odorant receptors (ORs). Principal Findings Compared with a fly's odorant receptors, MOx sensors from an electronic nose are on average more narrowly tuned but much more highly correlated with each other. A set of insect ORs can therefore sample broader regions of odorant space independently and redundantly than an equivalent number of MOx sensors. The comparison also highlights some important questions about the molecular nature of fly ORs. Conclusions The comparative approach generates practical learnings that may be taken up by solid-state physicists or engineers in designing new solid-state electronic nose sensors. It also potentially deepens our understanding of the performance of the biological system. PMID:19641604
NASA Astrophysics Data System (ADS)
Moustahfid, H.; Michaels, W.
2016-02-01
The vision of the US Integrated Ocean Observing System (U.S. IOOS) is to provide information and services to the nation for enhancing our understanding of the ecosystem and climate; sustaining living marine resources; improving public health and safety; reducing impacts of natural hazards and environmental changes; and expanding support for marine commerce and transportation. In the last decade, U.S. IOOS has made considerable progress in advancing physical and chemical observing systems, while further efforts are needed to fully integrate biological observing systems into U.S. IOOS. Recent technological advances in miniature, low power "bio" sensors deployed from fixed and mobile autonomous platforms enable remote sensing of biological components ranging from plankton greater than 20 micrometer with electro-optical technology to meso-zooplankton and nekton with hydroacoustic technology. Satellite communication linked to sensing technologies provide near real-time information of the movement and behavior of the biological organisms including the large marine predators. This opens up remarkable opportunities for observing the biotic realm at critical spatio-temporal scales for understanding how environmental changes impact on the productivity and health of our oceans. Biosensor technology has matured to be operationally integrated into ocean observation systems to provide synoptic bio-physical monitoring information. The operational objectives should be clearly defined and implemented by biological and physical oceanographers to optimize the integration of biological observing into U.S IOOS which will strengthen the national observing capabilities in response to the increasing demand for ecosystem observations to support ecosystem-based approaches for the sustainability of living marine resources and healthy oceans.
EDITORIAL: Sensors based on interfaces
NASA Astrophysics Data System (ADS)
Camassel, Jean; Soukiassian, Patrick G.
2007-12-01
Sensors are specific analog devices that convert a physical quantity, like the temperature or external pressure or concentration of carbon monoxide in a confined atmosphere, into an electrical signal. Considered in this way, every sensor is then a part of the artificial interface, which connects the human world to the world of machines. The other side of the interface is represented by actuators. Most often, after processing the data they are used to convert the out-coming electrical power into counteracting physical action. In the last few years, thanks to inexpensive silicon technology, enormous capability for data processing has been developed and the world of machines has become increasingly invasive. The world of sensors has become increasingly complex too. Applications range from classical measurements of the temperature, vibrations, shocks and acceleration to more recent chemical and bio-sensing technologies. Chemical sensors are used to detect the presence of specific, generally toxic, chemical species. To measure their concentration, one uses some specific property, generally a physical one, like the intensity of infrared absorption bands. Bio-sensors are new, more complex, devices that combine a bio-receptor with a physical transducer. The bio-receptor is a molecule (for instance, an enzyme like glucose oxidase) that can recognize a specific target (glucose molecules in the case of glucose oxidase). The enzyme must be fixed on the transducer and, as a consequence of recognition, the transducer must convert the event into a measurable analytical signal. A common feature of many chemical and bio-sensors is that they require a large surface of interaction with the outside world. For that reason and in order to increase efficiency, either nanoparticles or pores or a combination of both, made from various materials including (but not limited to) porous silicon, are often used as the functional transducer interface. The reviews in this Cluster Issue of Journal
In vitro bio-functionality of gallium nitride sensors for radiation biophysics.
Hofstetter, Markus; Howgate, John; Schmid, Martin; Schoell, Sebastian; Sachsenhauser, Matthias; Adigüzel, Denis; Stutzmann, Martin; Sharp, Ian D; Thalhammer, Stefan
2012-07-27
There is an increasing interest in the integration of hybrid bio-semiconductor systems for the non-invasive evaluation of physiological parameters. High quality gallium nitride and its alloys show promising characteristics to monitor cellular parameters. Nevertheless, such applications not only request appropriate sensing capabilities but also the biocompatibility and especially the biofunctionality of materials. Here we show extensive biocompatibility studies of gallium nitride and, for the first time, a biofunctionality assay using ionizing radiation. Analytical sensor devices are used in medical settings, as well as for cell- and tissue engineering. Within these fields, semiconductor devices have increasingly been applied for online biosensing on a cellular and tissue level. Integration of advanced materials such as gallium nitride into these systems has the potential to increase the range of applicability for a multitude of test devices and greatly enhance sensitivity and functionality. However, for such applications it is necessary to optimize cell-surface interactions and to verify the biocompatibility of the semiconductor. In this work, we present studies of mouse fibroblast cell activity grown on gallium nitride surfaces after applying external noxa. Cell-semiconductor hybrids were irradiated with X-rays at air kerma doses up to 250 mGy and the DNA repair dynamics, cell proliferation, and cell growth dynamics of adherent cells were compared to control samples. The impact of ionizing radiation on DNA, along with the associated cellular repair mechanisms, is well characterized and serves as a reference tool for evaluation of substrate effects. The results indicate that gallium nitride does not require specific surface treatments to ensure biocompatibility and suggest that cell signaling is not affected by micro-environmental alterations arising from gallium nitride-cell interactions. The observation that gallium nitride provides no bio-functional influence on
VUV-Photoionization CES-Detector of Volatile Bio-Marker Molecules
NASA Astrophysics Data System (ADS)
Mustafaev, Alexander; Luneva, Nataliya; Panasyuk, George; Timofeev, Nikolay; Tsyganov, Alexander
2014-10-01
Energy spectra of characteristic electrons released via photoionization by vacuum ultraviolet (VUV) radiation of admixture molecules in the atmospheric air, not using traditional evacuated energy analyzers, can be determined by Collisional Electron Spectroscopy (CES) method. Some details of CES-photoionization sensor were described in. Our further developments are devoted to application of CES-detectors for a mobile continuous bio-chemical diagnostics. It is known that ``on breathing'' it is possible to find out volatile bio-marker molecules of a lot of diseases (lung cancer, tuberculosis, COPD, asthma, diabetes, kidney disease, mammary cancer, Crohn's disease, ulcerative colitis, etc). But today's weighty and expensive laboratory equipment (like GC MS) provides observation of these bio-markers only during patients' visits to a doctor. In this way we study pocket-size CES-sensor with micro-plasma krypton resonance radiation source (10.6 eV photons) for the photoionization detection of metabolic ammonia, ethanol, acetone and pentane molecules directly in atmospheric air.
Bio-inspired sensor skins for structural health monitoring
NASA Astrophysics Data System (ADS)
Tata, Uday; Deshmukh, S.; Chiao, J. C.; Carter, Ronald; Huang, H.
2009-10-01
This paper presents the simulation and experimental work that proved the feasibility of using a patch antenna for strain measurement. A patch antenna, besides serving as a data transmitting device, can function as a transducer that directly encodes the strain experienced into its resonant frequency. Printed on a flexible substrate, the antenna sensor is small in size, has a low profile and can be conformal to any attached surface. The technique for interrogating the antenna sensor using a wireless non-contact method is also demonstrated. Without needing electric wiring for power supply and data transmitting, the antenna sensor has a great potential for the realization of engineered sensor skins that imitate the sense of pain for structural health monitoring purposes.
NASA Astrophysics Data System (ADS)
Goodwin, Thomas; Carr, Ryan; Mitra, Atindra K.; Selmic, Rastko R.
2009-05-01
We discuss the development of Position-Adaptive Sensors [1] for purposes for detecting embedded chemical substances in challenging environments. This concept is a generalization of patented Position-Adaptive Radar Concepts developed at AFRL for challenging conditions such as urban environments. For purposes of investigating the detection of chemical substances using multiple MAV (Micro-UAV) platforms, we have designed and implemented an experimental testbed with sample structures such as wooden carts that contain controlled leakage points. Under this general concept, some of the members of a MAV swarm can serve as external position-adaptive "transmitters" by blowing air over the cart and some of the members of a MAV swarm can serve as external position-adaptive "receivers" that are equipped with chemical or biological (chem/bio) sensors that function as "electronic noses". The objective can be defined as improving the particle count of chem/bio concentrations that impinge on a MAV-based position-adaptive sensor that surrounds a chemical repository, such as a cart, via the development of intelligent position-adaptive control algorithms. The overall effect is to improve the detection and false-alarm statistics of the overall system. Within the major sections of this paper, we discuss a number of different aspects of developing our initial MAV-Based Sensor Testbed. This testbed includes blowers to simulate position-adaptive excitations and a MAV from Draganfly Innovations Inc. with stable design modifications to accommodate our chem/bio sensor boom design. We include details with respect to several critical phases of the development effort including development of the wireless sensor network and experimental apparatus, development of the stable sensor boom for the MAV, integration of chem/bio sensors and sensor node onto the MAV and boom, development of position-adaptive control algorithms and initial tests at IDCAST (Institute for the Development and
Bio-Functional Au/Si Nanorods for Pathogen Detection
USDA-ARS?s Scientific Manuscript database
Technical Abstract Nanotechnology applications for food safety and biosecurity, especially development of nanoscale sensors for foodborne pathogen measurement are emerging. A novel bio-functional nanosensor for Salmonella detection was developed using hetero-nanorods. The silica nanorods were fabr...
Linares, Robert; Doering, Patrick; Linares, Bryant
2009-01-01
The use of diamond for advanced applications has been the dream of mankind for centuries. Until recently this dream has been realized only in the use of diamond for gemstones and abrasive applications where tons of diamonds are used on an annual basis. Diamond is the material system of choice for many applications, but its use has historically been limited due to the small size, high cost, and inconsistent (and typically poor) quality of available diamond materials until recently. The recent development of high quality, single crystal diamond crystal growth via the Chemical Vapor Deposition (CVD) process has allowed physcists and increasingly scientists in the life science area to think beyond these limitations and envision how diamond may be used in advanced applications ranging from quantum computing, to power generation and molecular imaging, and eventually even diamond nano-bots. Because of diamond's unique properties as a bio-compatible material, better understanding of diamond's quantum effects and a convergence of mass production, semiconductor-like fabrication process, diamond now promises a unique and powerful key to the realization of the bio-electronic devices being envisioned for the new era of medical science. The combination of robust in-the-body diamond based sensors, coupled with smart bio-functionalized diamond devices may lead to diamond being the platform of choice for bio-electronics. This generation of diamond based bio-electronic devices would contribute substantially to ushering in a paradigm shift for medical science, leading to vastly improved patient diagnosis, decrease of drug development costs and risks, and improved effectiveness of drug delivery and gene therapy programs through better timed and more customized solutions.
Scales of variability of bio-optical properties as observed from near-surface drifters
NASA Technical Reports Server (NTRS)
Abbott, Mark R.; Brink, Kenneth H.; Booth, C. R.; Blasco, Dolors; Swenson, Mark S.; Davis, Curtiss O.; Codispoti, L. A.
1995-01-01
A drifter equipped with bio-optical sensors and an automated water sampler was deployed in the California Current as part of the coastal transition zone program to study the biological, chemical, and physical dynamics of the meandering filaments. During deployments in 1987 and 1988, measurements were made of fluorescence, downwelling irradiance, upwelling radiance, and beam attenuation using several bio-optical sensors. Samples were collected by an automated sampler for later analysis of nutrients and phytoplankton species compositon. Large-scale spatial and temporal changes in the bio-optical and biological properties of the region were driven by changes in phytoplankton species composition which, in turn, were associated with the meandering circulation. Variance spectra of the bio-optical paramenters revealed fluctuations on both diel and semidiurnal scales, perhaps associated with solar variations and internal tides, respectively. Offshore, inertial-scale fluctuations were apparent in the variance spectra of temperature, fluorescence, and beam attenuation. Although calibration samples can help remove some of these variations, these results suggest that the use of bio-optical data from unattended platforms such as moorings and drifters must be analyzed carefully. Characterization of the scaled of phytoplankton variability must account for the scales of variability in the algorithms used to convert bio-optical measurments into biological quantities.
NASA Astrophysics Data System (ADS)
Arefin, Md Shamsul; Porter, Timothy L.
2012-03-01
This work reports on the behavior of piezoresistive microcantilever sensors under optimizing conditions of ac electroosmotic enhancement. Piezoresistive microcantilevers are used as sensor elements for detection of concentrated bio-particles. Without preconcentrating the samples, using ac electroosmosis, these bio-particles have been manipulated onto the piezoresistive microcantilever. A piezoresistive microcantilever senses the dimensional changes upon particle exposure as a resistance change. This paper represents the integration of ac electroosmosis with a piezoresistive micro-cantilever sensor for the detection of bio-particles. A working prototype is presented here, and the experiments are conducted on Herpes Simplex type-1 virus (HSV-1) and Escherichia Coli (E. coli) bacteria.
NASA Astrophysics Data System (ADS)
Sajid, Memoon; Aziz, Shahid; Kim, Go Bum; Kim, Soo Wan; Jo, Jeongdai; Choi, Kyung Hyun
2016-07-01
A bio-compatible disposable organic humidity sensor has been fabricated that can be transferred to any arbitrary target surface. Single cell thick onion membrane has been used as the substrate while it also doubles as the active layer of the sensor. Two different types of sensors were fabricated. In type-1, the membrane was fixed into a plastic frame with IDT patterns on one side while the other side was also exposed to environment. In type-2, onion membrane was attached to a glass substrate with one side exposed to environment having an IDT screen-printed on top of it. The electrical output response of the sensors showed their ability to detect relative humidity between 0% RH and 80% RH with stable response and good sensitivity. The impedance of the sensors changed from 16 MΩ to 2 MΩ for type-1 and 6 MΩ to 20 KΩ for type-2. The response times of type-1 and type-2 were ~1 and 1.5 seconds respectively. The recovery times were ~10.75 seconds and ~11.25 seconds for type-1 and type-2 respectively. The device was successfully transferred to various randomly shaped surfaces without damaging the device.
Miniature Wireless BioSensor for Remote Endoscopic Monitoring
NASA Astrophysics Data System (ADS)
Nemiroski, Alex; Brown, Keith; Issadore, David; Westervelt, Robert; Thompson, Chris; Obstein, Keith; Laine, Michael
2009-03-01
We have built a miniature wireless biosensor with fluorescence detection capability that explores the miniaturization limit for a self-powered sensor device assembled from the latest off-the-shelf technology. The device is intended as a remote medical sensor to be inserted endoscopically and remainin a patient's gastrointestinal tract for a period of weeks, recording and transmitting data as necessary. A sensing network may be formed by using multiple such devices within the patient, routing information to an external receiver that communicates through existing mobilephone networks to relay data remotely. By using a monolithic IC chip with integrated processor, memory, and 2.4 GHz radio,combined with a photonic sensor and miniature battery, we have developed a fully functional computing device in a form factorcompliantwith insertion through the narrowest endoscopic channels (less than 3mm x 3mm x 20mm). We envision similar devices with various types of sensors to be used in many different areas of the human body.
Tan, Y; Yin, J; Liang, C; Peng, H; Nie, L; Yao, S
2001-03-01
A new bio-mimetic quartz crystal thickness-shear-mode (TSM) sensor, using an imprinted polymer coating as the sensitive material, has been fabricated and applied to the determination of nicotine (NIC) in human serum and urine. The molecularly imprinted polymer (MIP) was synthesized using NIC as the template molecule and methacrylic acid (MAA) as the functional monomer. The sensor showed high selectivity and a sensitive response to NIC in aqueous system. The linear response range of the sensor was between 5.0 x 10(-8) and 1.0 x 10(-4) M with a detection limit of 2.5 x 10(-8) M. The viscoelasticity of the coating in the air and in liquid has been studied by the impedance spectrum. The MIP sensor was stable and exhibited effective reproducibility. Satisfactory results were achieved in the detection of the real samples.
An aptamer-based fluorescence bio-sensor for chiral recognition of arginine enantiomers
NASA Astrophysics Data System (ADS)
Yuan, Haiyan; Huang, Yunmei; Yang, Jidong; Guo, Yuan; Zeng, Xiaoqing; Zhou, Shang; Cheng, Jiawei; Zhang, Yuhui
2018-07-01
In this study, a novel aptamer - based fluorescence bio-sensor (aptamer-AuNps) was developed for chiral recognition of arginine (Arg) enantiomers based on aptamer and gold nanoparticles (AuNps). Carboxyfluorescein (FAM) labeled aptamers (Apt) were absorbed on AuNps and their fluorescence intensity could be significantly quenched by AuNps based on fluorescence resonance energy transfer (FRET). Once D-Arg or L-Arg were added into the above solution, the aptamer specifically bind to Arg enantiomers and released from AuNps, so the fluorescence intensity of D-Arg system and L-Arg system were all enhanced. The affinity of Apt to L-Arg is tighter to D-Arg, so the enhanced fluorescence signals of L-Arg system was stronger than D-Arg system. What's more, the enhanced fluorescence were directly proportional to the concentration of D-Arg and L-Arg ranging from 0-300 nM and 0-400 nM with related coefficients of 0.9939 and 0.9952, respectively. Furthermore, the method was successfully applied to detection L-Arg in human urine samples with satisfactory results. Eventually, a simple "OR" logic gate with D-Arg &L-Arg as inputs and AuNps aggregation state as outputs was fabricated, which can help us understand the chiral recognition process deeply.
NASA Astrophysics Data System (ADS)
Hanson, David F.
2017-04-01
Bio-inspired intelligent robots are coming of age in both research and industry, propelling market growth for robots and A.I. However, conventional motors limit bio-inspired robotics. EAP actuators and sensors could improve the simplicity, compliance, physical scaling, and offer bio-inspired advantages in robotic locomotion, grasping and manipulation, and social expressions. For EAP actuators to realize their transformative potential, further innovations are needed: the actuators must be robust, fast, powerful, manufacturable, and affordable. This presentation surveys progress, opportunities, and challenges in the author's latest work in social robots and EAP actuators, and proposes a roadmap for EAP actuators in bio-inspired intelligent robotics.
An aptamer-based fluorescence bio-sensor for chiral recognition of arginine enantiomers.
Yuan, Haiyan; Huang, Yunmei; Yang, Jidong; Guo, Yuan; Zeng, Xiaoqing; Zhou, Shang; Cheng, Jiawei; Zhang, Yuhui
2018-07-05
In this study, a novel aptamer - based fluorescence bio-sensor (aptamer-AuNps) was developed for chiral recognition of arginine (Arg) enantiomers based on aptamer and gold nanoparticles (AuNps). Carboxyfluorescein (FAM) labeled aptamers (Apt) were absorbed on AuNps and their fluorescence intensity could be significantly quenched by AuNps based on fluorescence resonance energy transfer (FRET). Once d-Arg or l-Arg were added into the above solution, the aptamer specifically bind to Arg enantiomers and released from AuNps, so the fluorescence intensity of d-Arg system and l-Arg system were all enhanced. The affinity of Apt to l-Arg is tighter to d-Arg, so the enhanced fluorescence signals of l-Arg system was stronger than d-Arg system. What's more, the enhanced fluorescence were directly proportional to the concentration of d-Arg and l-Arg ranging from 0-300 nM and 0-400 nM with related coefficients of 0.9939 and 0.9952, respectively. Furthermore, the method was successfully applied to detection l-Arg in human urine samples with satisfactory results. Eventually, a simple "OR" logic gate with d-Arg &l-Arg as inputs and AuNps aggregation state as outputs was fabricated, which can help us understand the chiral recognition process deeply. Copyright © 2018 Elsevier B.V. All rights reserved.
Silicon nano-membrane based photonic crystal microcavities for high sensitivity bio-sensing.
Lai, Wei-Cheng; Chakravarty, Swapnajit; Zou, Yi; Chen, Ray T
2012-04-01
We experimentally demonstrated photonic crystal microcavity based resonant sensors coupled to photonic crystal waveguides in silicon nano-membrane on insulator for chemical and bio-sensing. Linear L-type microcavities are considered. In contrast to cavities with small mode volumes, but low quality factors for bio-sensing, we showed increasing the length of the microcavity enhances the quality factor of the resonance by an order of magnitude and increases the resonance wavelength shift while retaining compact device characteristics. Q~26760 and sensitivity down to 15 ng/ml and ~110 pg/mm2 in bio-sensing was experimentally demonstrated on silicon-on-insulator devices.
Bio-logging of physiological parameters in higher marine vertebrates
NASA Astrophysics Data System (ADS)
Ponganis, Paul J.
2007-02-01
Bio-logging of physiological parameters in higher marine vertebrates had its origins in the field of bio-telemetry in the 1960s and 1970s. The development of microprocessor technology allowed its first application to bio-logging investigations of Weddell seal diving physiology in the early 1980s. Since that time, with the use of increased memory capacity, new sensor technology, and novel data processing techniques, investigators have examined heart rate, temperature, swim speed, stroke frequency, stomach function (gastric pH and motility), heat flux, muscle oxygenation, respiratory rate, diving air volume, and oxygen partial pressure (P) during diving. Swim speed, heart rate, and body temperature have been the most commonly studied parameters. Bio-logging investigation of pressure effects has only been conducted with the use of blood samplers and nitrogen analyses on animals diving at isolated dive holes. The advantages/disadvantages and limitations of recording techniques, probe placement, calibration techniques, and study conditions are reviewed.
Hammoud, Abbas; Chamseddine, Ahmad; Nguyen, Dang K; Sawan, Mohamad
2016-08-01
The need of continuous real-time monitoring device for in-vivo drug level detection has been widely articulated lately. Such monitoring could guide drug posology and timing of intake, detect low or high drug levels, in order to take adequate measures, and give clinicians a valuable window into patients' health and their response to therapeutics. This paper presents a novel implantable bio-sensor based on impedance measurement capable of continuously monitoring various antiepileptic drug levels. This portable point-of-care microsystem replaces large and stationary conventional macrosystems, and is a one of a kind system designed with an array of electrodes to monitor various anti-epileptic drugs rather than one drug. The micro-system consists of (i) the front-end circuit including an inductive coil to receive energy from an external base station, and to exchange data with the latter; (ii) the power management block; (iii) the readout and control block; and (iv) the biosensor array. The electrical circuitry was designed using the 0.18-um CMOS process technology intended to be miniature and consume ultra-low power.
Dissolvable tattoo sensors: from science fiction to a viable technology
NASA Astrophysics Data System (ADS)
Cheng, Huanyu; Yi, Ning
2017-01-01
Early surrealistic painting and science fiction movies have envisioned dissolvable tattoo electronic devices. In this paper, we will review the recent advances that transform that vision into a viable technology, with extended capabilities even beyond the early vision. Specifically, we focus on the discussion of a stretchable design for tattoo sensors and degradable materials for dissolvable sensors, in the form of inorganic devices with a performance comparable to modern electronics. Integration of these two technologies as well as the future developments of bio-integrated devices is also discussed. Many of the appealing ideas behind developments of these devices are drawn from nature and especially biological systems. Thus, bio-inspiration is believed to continue playing a key role in future devices for bio-integration and beyond.
Astronaut Bonnie Dunbar preparing to perform bio-medical test
1985-10-30
61A-18-001A (30 Oct-6 Nov 1985) --- Her head equipped with a sensor device, astronaut Bonnie J. Dunbar, 61-A mission specialist, talks to earthbound investigators while participating in a bio-medical test. A 35mm camera was used to expose the frame.
Bio-Inspired Networking — Self-Organizing Networked Embedded Systems
NASA Astrophysics Data System (ADS)
Dressler, Falko
The turn to nature has brought us many unforeseen great concepts and solutions. This course seems to hold on for many research domains. In this article, we study the applicability of biological mechanisms and techniques in the domain of communications. In particular, we study the behavior and the challenges in networked embedded systems that are meant to self-organize in large groups of nodes. Application examples include wireless sensor networks and sensor/actuator networks. Based on a review of the needs and requirements in such networks, we study selected bio-inspired networking approaches that claim to outperform other methods in specific domains. We study mechanisms in swarm intelligence, the artificial immune system, and approaches based on investigations on the cellular signaling pathways. As a major conclusion, we derive that bio-inspired networking techniques do have advantages compared to engineering methods. Nevertheless, selection and employment must be done carefully to achieve the desired performance gains.
Monitoring gait in multiple sclerosis with novel wearable motion sensors.
Moon, Yaejin; McGinnis, Ryan S; Seagers, Kirsten; Motl, Robert W; Sheth, Nirav; Wright, John A; Ghaffari, Roozbeh; Sosnoff, Jacob J
2017-01-01
Mobility impairment is common in people with multiple sclerosis (PwMS) and there is a need to assess mobility in remote settings. Here, we apply a novel wireless, skin-mounted, and conformal inertial sensor (BioStampRC, MC10 Inc.) to examine gait characteristics of PwMS under controlled conditions. We determine the accuracy and precision of BioStampRC in measuring gait kinematics by comparing to contemporary research-grade measurement devices. A total of 45 PwMS, who presented with diverse walking impairment (Mild MS = 15, Moderate MS = 15, Severe MS = 15), and 15 healthy control subjects participated in the study. Participants completed a series of clinical walking tests. During the tests participants were instrumented with BioStampRC and MTx (Xsens, Inc.) sensors on their shanks, as well as an activity monitor GT3X (Actigraph, Inc.) on their non-dominant hip. Shank angular velocity was simultaneously measured with the inertial sensors. Step number and temporal gait parameters were calculated from the data recorded by each sensor. Visual inspection and the MTx served as the reference standards for computing the step number and temporal parameters, respectively. Accuracy (error) and precision (variance of error) was assessed based on absolute and relative metrics. Temporal parameters were compared across groups using ANOVA. Mean accuracy±precision for the BioStampRC was 2±2 steps error for step number, 6±9ms error for stride time and 6±7ms error for step time (0.6-2.6% relative error). Swing time had the least accuracy±precision (25±19ms error, 5±4% relative error) among the parameters. GT3X had the least accuracy±precision (8±14% relative error) in step number estimate among the devices. Both MTx and BioStampRC detected significantly distinct gait characteristics between PwMS with different disability levels (p<0.01). BioStampRC sensors accurately and precisely measure gait parameters in PwMS across diverse walking impairment levels and detected
Monitoring gait in multiple sclerosis with novel wearable motion sensors
McGinnis, Ryan S.; Seagers, Kirsten; Motl, Robert W.; Sheth, Nirav; Wright, John A.; Ghaffari, Roozbeh; Sosnoff, Jacob J.
2017-01-01
Background Mobility impairment is common in people with multiple sclerosis (PwMS) and there is a need to assess mobility in remote settings. Here, we apply a novel wireless, skin-mounted, and conformal inertial sensor (BioStampRC, MC10 Inc.) to examine gait characteristics of PwMS under controlled conditions. We determine the accuracy and precision of BioStampRC in measuring gait kinematics by comparing to contemporary research-grade measurement devices. Methods A total of 45 PwMS, who presented with diverse walking impairment (Mild MS = 15, Moderate MS = 15, Severe MS = 15), and 15 healthy control subjects participated in the study. Participants completed a series of clinical walking tests. During the tests participants were instrumented with BioStampRC and MTx (Xsens, Inc.) sensors on their shanks, as well as an activity monitor GT3X (Actigraph, Inc.) on their non-dominant hip. Shank angular velocity was simultaneously measured with the inertial sensors. Step number and temporal gait parameters were calculated from the data recorded by each sensor. Visual inspection and the MTx served as the reference standards for computing the step number and temporal parameters, respectively. Accuracy (error) and precision (variance of error) was assessed based on absolute and relative metrics. Temporal parameters were compared across groups using ANOVA. Results Mean accuracy±precision for the BioStampRC was 2±2 steps error for step number, 6±9ms error for stride time and 6±7ms error for step time (0.6–2.6% relative error). Swing time had the least accuracy±precision (25±19ms error, 5±4% relative error) among the parameters. GT3X had the least accuracy±precision (8±14% relative error) in step number estimate among the devices. Both MTx and BioStampRC detected significantly distinct gait characteristics between PwMS with different disability levels (p<0.01). Conclusion BioStampRC sensors accurately and precisely measure gait parameters in PwMS across diverse walking
Optical bio-sniffer for ethanol vapor using an oxygen-sensitive optical fiber.
Mitsubayashi, Kohji; Kon, Takuo; Hashimoto, Yuki
2003-11-30
An optical bio-sniffer for ethanol was constructed by immobilizing alcohol oxidase (AOD) onto a tip of a fiber optic oxygen sensor with a tube-ring, using an oxygen sensitive ruthenium organic complex (excitation, 470 nm; fluorescent, 600 nm). A reaction unit for circulating buffer solution was applied to the tip of the device. After the experiment in the liquid phase, the sniffer-device was applied for gas analysis using a gas flow measurement system with a gas generator. The optical device was applied to detect the oxygen consumption induced by AOD enzymatic reaction with alcohol application. The sensor in the liquid phase was used to measure ethanol solution from 0.50 to 9.09 mmol/l. Then, the bio-sniffer was calibrated against ethanol vapor from 0.71 to 51.49 ppm with good gas-selectivity based on the AOD substrate specificity. The bio-sniffer with the reaction unit was also used to monitor the concentration change of gaseous ethanol by rinsing and cleaning the fiber tip and the enzyme membrane with buffer solution.
Seong-Jin Kim; Euisik Yoon
2012-06-01
We present a label-free CMOS field-effect transistor sensing array to detect the surface potential change affected by the negative charge in DNA molecules for real-time monitoring and quantification. The proposed CMOS bio sensor includes a new sensing pixel architecture implemented with correlated double sampling for reducing offset fixed pattern noise and 1/f noise of the sensing devices. We incorporated non-surface binding detection which allows real-time continuous monitoring of DNA concentrations without immobilizing them on the sensing surface. Various concentrations of 19-bp oligonucleotides solution can be discriminated using the prototype device fabricated in 1- μm double-poly double-metal standard CMOS process. The detection limit was measured as 1.1 ng/μl with a dynamic range of 40 dB and the transient response time was measured less than 20 seconds.
Design of Inkjet-Printed RFID-Based Sensor on Paper: Single- and Dual-Tag Sensor Topologies.
Kim, Sangkil; Georgiadis, Apostolos; Tentzeris, Manos M
2018-06-17
The detailed design considerations for the printed RFID-based sensor system is presented in this paper. Starting from material selection and metallization method, this paper discusses types of RFID-based sensors (single- & dual-tag sensor topologies), design procedures, and performance evaluation methods for the wireless sensor system. The electrical properties of the paper substrates (cellulose-based and synthetic papers) and the silver nano-particle-based conductive film are thoroughly characterized for RF applications up to 8 GHz. The reported technology could potentially set the foundation for truly “green”, low-cost, scalable wireless topologies for autonomous Internet-of-Things (IoT), bio-monitoring, and “smart skin” applications.
Nano ZnO embedded in Chitosan matrix for vibration sensor application
NASA Astrophysics Data System (ADS)
Praveen, E.; Murugan, S.; Jayakumar, K.
2015-06-01
Biopolymer Chitosan is embedded with various concentration of ZnO nano particle and such a bio-nano composite electret has been fabricated by casting method. The morphological, structural, optical and electrical characterization of the bio-nano composite electret film have been carried out. Isolation and piezoelectric measurements of bio-nano composite have also been carried out indicating the possibility of using it as a mechanical sensor element.
Molecular Imprinting Technology in Quartz Crystal Microbalance (QCM) Sensors.
Emir Diltemiz, Sibel; Keçili, Rüstem; Ersöz, Arzu; Say, Rıdvan
2017-02-24
Molecularly imprinted polymers (MIPs) as artificial antibodies have received considerable scientific attention in the past years in the field of (bio)sensors since they have unique features that distinguish them from natural antibodies such as robustness, multiple binding sites, low cost, facile preparation and high stability under extreme operation conditions (higher pH and temperature values, etc.). On the other hand, the Quartz Crystal Microbalance (QCM) is an analytical tool based on the measurement of small mass changes on the sensor surface. QCM sensors are practical and convenient monitoring tools because of their specificity, sensitivity, high accuracy, stability and reproducibility. QCM devices are highly suitable for converting the recognition process achieved using MIP-based memories into a sensor signal. Therefore, the combination of a QCM and MIPs as synthetic receptors enhances the sensitivity through MIP process-based multiplexed binding sites using size, 3D-shape and chemical function having molecular memories of the prepared sensor system toward the target compound to be detected. This review aims to highlight and summarize the recent progress and studies in the field of (bio)sensor systems based on QCMs combined with molecular imprinting technology.
Molecular Imprinting Technology in Quartz Crystal Microbalance (QCM) Sensors
Emir Diltemiz, Sibel; Keçili, Rüstem; Ersöz, Arzu; Say, Rıdvan
2017-01-01
Molecularly imprinted polymers (MIPs) as artificial antibodies have received considerable scientific attention in the past years in the field of (bio)sensors since they have unique features that distinguish them from natural antibodies such as robustness, multiple binding sites, low cost, facile preparation and high stability under extreme operation conditions (higher pH and temperature values, etc.). On the other hand, the Quartz Crystal Microbalance (QCM) is an analytical tool based on the measurement of small mass changes on the sensor surface. QCM sensors are practical and convenient monitoring tools because of their specificity, sensitivity, high accuracy, stability and reproducibility. QCM devices are highly suitable for converting the recognition process achieved using MIP-based memories into a sensor signal. Therefore, the combination of a QCM and MIPs as synthetic receptors enhances the sensitivity through MIP process-based multiplexed binding sites using size, 3D-shape and chemical function having molecular memories of the prepared sensor system toward the target compound to be detected. This review aims to highlight and summarize the recent progress and studies in the field of (bio)sensor systems based on QCMs combined with molecular imprinting technology. PMID:28245588
PREFACE: 8th Ibero-American Congress on Sensors (IBERSENSOR 2012)
NASA Astrophysics Data System (ADS)
Ramos, Idalia; Santiago-Avilés, Jorge J.
2013-03-01
The 8th Ibero-American Congress on Sensors (IBERSENSOR 2012) was held in Carolina, Puerto Rico on 16-19 October 2012. IBERSENSOR is a forum of the Spanish and Portuguese speaking scientific community, working in the fields of sensors of every possible kind and their applications. Previous conferences in the series were successfully carried out in La Habana, Cuba (1998); Buenos Aires, Argentina (2000); Lima, Perú (2002); Puebla, México (2004); Montevideo, Uruguay (2006); Sao Paulo, Brasil (2008) and Lisboa, Portugal (2010). IBERSENSOR 2012 participants included researchers from eleven countries in the Americas and Europe, in particular young men and women. The conference was organized and sponsored by the Partnership for Research and Education in Materials (NSF-DMR-0934195) a collaborative program between the University of Puerto Rico at Humacao (UPRH) and the University of Pennsylvania (PENN) Materials Research Science and Engineering Center, sponsored by the USA National Science Foundation (NSF). Other sponsors included the Center for Advanced Nanoscale Materials of the University of Puerto Rico at Río Piedras and the Nano/Bio Interface Center (NBIC) at PENN. The Proceedings of IBERSENSOR 2012 include a selection of 21 research papers in the areas of Materials and Processes for Sensor Development, Nano-Sensors, Chemical Sensors, Mechanical Sensors, Optical Sensors, Wireless Sensors, Sensor signal conditioning and Instrumentation, Microfluidic Devices, and Biomedical and Environmental Applications. Editors Idalia Ramos University of Puerto Rico at Humacao, Puerto Rico Jorge J Santiago-Avilés University of Pennsylvania, USA Group photograph Logos Ibero-American Congress on Sensors Ibero-American Congress on Sensors (Ibersensor) Main Sponsors PENN-UPRH-PREM Partnership for Research and Education in Materials (PENN-UPRH-PREM) University of Puerto Rico at Humacao USA National Science Foundation USA National Science Foundation Other Sponsors Center for Advanced
The non-contact biometric identified bio signal measurement sensor and algorithms.
Kim, Chan-Il; Lee, Jong-Ha
2018-01-01
In these days, wearable devices have been developed for effectively measuring biological data. However, these devices have tissue allege and noise problem. To solve these problems, biometric measurement based on a non-contact method, such as face image sequencing is developed. This makes it possible to measure biometric data without any operation and side effects. However, it is impossible for a remote center to identify the person whose data are measured by the novel methods. In this paper, we propose the novel non-contact heart rate and blood pressure imaging system, Deep Health Eye. This system has authentication process at the same time as measuring bio signals, through non-contact method. In the future, this system can be convenient home bio signal monitoring system by combined with smart mirror.
Programmable Nano-Bio-Chip Sensors: Analytical Meets Clinical
Jokerst, Jesse V.; Floriano, Pierre N.; Christodoulides, Nicolaos; McDevitt, John T.; Jacobson, James W.; Bhagwandin, Bryon D.
2010-01-01
synopsis There have been many recent advances in the nano-bio-chip (NBC) analysis methodology with implications for a number of high-morbidity diseases including HIV, cancer, and heart disease. In their Feature article, Jesse V. Jokerst of The University of Texas at Austin; Pierre N. Floriano, Nicolaos Christodoulides, and John T. McDevitt of Rice University; and James W. Jacobson and Bryon D. Bhagwandin of LabNow, Inc. discuss the construction, capabilities, and advantages of NBCs. The cover shows arrays of NBCs. Images courtesy of Glennon Simmons/McDevitt Lab and Marcha Miller of The University of Texas at Austin. PMID:20128622
Photonic crystal sensors: Physics and applications
NASA Astrophysics Data System (ADS)
Dinodiya, Sapna; Suthar, B.; Bhargava, A.
2018-05-01
Photonic sensors have evolved rapidly in last few decades because of the major requirement of sensing applications in optical communication and biomedical diagnostics. Photonic crystals are of tremendous use in designing photonic sensors. Any physical phenomenon, for instance temperature, pressure, strain, and presence of chemicals and bio molecules that can alter periodicity and refractive index of structure of the photonic crystal, can be sensed by measuring optical properties like spectral pattern of reflected and transmitted power. The physics and applications of various photonic crystal sensors are presented in this article.
BioFET-SIM web interface: implementation and two applications.
Hediger, Martin R; Jensen, Jan H; De Vico, Luca
2012-01-01
We present a web interface which allows us to conveniently set up calculations based on the BioFET-SIM model. With the interface, the signal of a BioFET sensor can be calculated depending on its parameters, as well as the signal dependence on pH. As an illustration, two case studies are presented. In the first case, a generic peptide with opposite charges on both ends is inverted in orientation on a semiconducting nanowire surface leading to a corresponding change in sign of the computed sensitivity of the device. In the second case, the binding of an antibody/antigen complex on the nanowire surface is studied in terms of orientation and analyte/nanowire surface distance. We demonstrate how the BioFET-SIM web interface can aid in the understanding of experimental data and postulate alternative ways of antibody/antigen orientation on the nanowire surface.
Electrochemical (Bio) Sensors for Environmental and Food Analyses.
Honeychurch, Kevin C; Piano, Martina
2018-06-22
In recent years, great progress has been made in the development of sensors and biosensors to meet the demands of environmental and food analysis. In this Special Issue, the state of art and the future trends in the field of environmental and food analyses have been explored. A total of seven papers (three research and four review papers) are included. These are focused on the fabrication and detection of contaminates such as heavy metals, pesticides and food components, including uric acid and 3-hydroxybutyrate. Included in this Issue is a paper dedicated to the experimental determination of the electroactive area of screen-printed electrodes, an important parameter in the development of such sensors.
Continuous Time Level Crossing Sampling ADC for Bio-Potential Recording Systems
Tang, Wei; Osman, Ahmad; Kim, Dongsoo; Goldstein, Brian; Huang, Chenxi; Martini, Berin; Pieribone, Vincent A.
2013-01-01
In this paper we present a fixed window level crossing sampling analog to digital convertor for bio-potential recording sensors. This is the first proposed and fully implemented fixed window level crossing ADC without local DACs and clocks. The circuit is designed to reduce data size, power, and silicon area in future wireless neurophysiological sensor systems. We built a testing system to measure bio-potential signals and used it to evaluate the performance of the circuit. The bio-potential amplifier offers a gain of 53 dB within a bandwidth of 200 Hz-20 kHz. The input-referred rms noise is 2.8 µV. In the asynchronous level crossing ADC, the minimum delta resolution is 4 mV. The input signal frequency of the ADC is up to 5 kHz. The system was fabricated using the AMI 0.5 µm CMOS process. The chip size is 1.5 mm by 1.5 mm. The power consumption of the 4-channel system from a 3.3 V supply is 118.8 µW in the static state and 501.6 µW with a 240 kS/s sampling rate. The conversion efficiency is 1.6 nJ/conversion. PMID:24163640
Chen, Kuan-Ting; Chang, Chin-Kai; Kuo, Hui-Lung; Lee, Chih-Kung
2011-01-01
We integrated a piezoelectric sensing layer lamina containing liquid crystals (LC) and spiropyran (SP) in a LC/SP mixture to create an optically reconfigurable modal sensor for a cantilever beam. The impedance of this LC/SP lamina was decreased by UV irradiation which constituted the underlying mechanism to modulate the voltage externally applied to the piezoelectric actuating layer. Illuminating a specific pattern onto the LC/SP lamina provided us with a way to spatially modulate the piezoelectric vibration signal. We showed that if an UV illuminated pattern matches the strain distribution of a specific mode, a piezoelectric modal sensor can be created. Since UV illumination can be changed in situ in real-time, our results confirm for the first time since the inception of smart sensors, that an optically tailored modal sensor can be created. Some potential applications of this type of sensor include energy harvesting devices, bio-chips, vibration sensing and actuating devices.
NASA Astrophysics Data System (ADS)
Bundke, U.; Nillius, B.; Bingemer, H.; Curtius, J.
2012-04-01
We have designed the BIO-IN detector as part of the ice nucleus counter FINCH (Fast Ice Nuclei CHamber counter) to distinguish activated Ice Nuclei (IN) ice crystals from water droplets (CCN) (Bundke et al. 2008) and their fraction of biological origin (Bundke 2010). The modified BIO-IN sensor illuminates an aerosol stream with a 405 nm laser, replacing a 365nm LED of the original BIO IN design. Particles will scatter the light and those of biological origin will show intrinsic fluorescence emissions by excitation of mainly Riboflavin, also known as vitamin B2. The incident laser light is circularly polarized by introducing a quarter-wave-plate. The circular depolarization ratio (p44/p11) of the scattering matrix is measured in the backward direction by two photomultipliers at 110° scattering angle using a combination of quarter-wave-plate and a beam splitting cube to analyze the two circular polarization components. The detection limit was lowered towards particle size of about 400nm diameter (non activated particles). It is now possible to calculate the activated fraction of IN of biological origin with respect to all biological particles measured with one detector. The performance of the sensor will be demonstrated showing the circular- depolarization properties of different test aerosol, dust samples, volcanic ashes as well as different biological particles. Measurements on the mountain Puy de Dôme of IN number concentration of ambient air, as well as measurements at the AIDA facility in Karlsruhe of the IN activation curves from different bacteria are shown. Acknowledgements: This work was supported by the German Research Foundation, Grant: BU 1432/3-2 BU 1432/4-1
Decoding static and dynamic arm and hand gestures from the JPL BioSleeve
NASA Astrophysics Data System (ADS)
Wolf, M. T.; Assad, C.; Stoica, A.; You, Kisung; Jethani, H.; Vernacchia, M. T.; Fromm, J.; Iwashita, Y.
This paper presents methods for inferring arm and hand gestures from forearm surface electromyography (EMG) sensors and an inertial measurement unit (IMU). These sensors, together with their electronics, are packaged in an easily donned device, termed the BioSleeve, worn on the forearm. The gestures decoded from BioSleeve signals can provide natural user interface commands to computers and robots, without encumbering the users hands and without problems that hinder camera-based systems. Potential aerospace applications for this technology include gesture-based crew-autonomy interfaces, high degree of freedom robot teleoperation, and astronauts' control of power-assisted gloves during extra-vehicular activity (EVA). We have developed techniques to interpret both static (stationary) and dynamic (time-varying) gestures from the BioSleeve signals, enabling a diverse and adaptable command library. For static gestures, we achieved over 96% accuracy on 17 gestures and nearly 100% accuracy on 11 gestures, based solely on EMG signals. Nine dynamic gestures were decoded with an accuracy of 99%. This combination of wearableEMGand IMU hardware and accurate algorithms for decoding both static and dynamic gestures thus shows promise for natural user interface applications.
Iijima, Masumi; Yoshimoto, Nobuo; Niimi, Tomoaki; Maturana, Andrés D; Kuroda, Shun'ichi
2016-06-01
Mammalian receptors are recognized as target molecules for drug discovery, and chemical libraries have been screened for both potential antagonists and agonists mainly by ligand-binding assays using immobilized receptors. A bio-nanocapsule (BNC) of approximately 30 nm that displays a tandem form of the protein A-derived immunoglobulin G (IgG) Fc-binding Z domains (denoted as ZZ-BNC) has been developed for both clustering and oriented immobilization of IgGs on the solid phase of immunosensors. In this study, human IgG1 Fc-fused vascular endothelial growth factor (VEGF) receptor was immobilized through ZZ-BNC on the sensor chip of quartz crystal microbalance (ZZ-BNC-coating). When compared with direct adsorption and protein A-coating, the sensor chip showed higher sensitivity (∽46- and ∽165-fold, respectively) and larger ligand-binding capacity (∽4- and ∽18-fold, respectively). Furthermore, the number of VEGF molecules bound to its receptor increased from 0.20 (direct adsorption) to 2.06 by ZZ-BNC-coating, strongly suggesting that ZZ-BNC reduced the steric hindrance near ligand recognition sites through oriented immobilization. Similarly, the sensitivity and ligand-binding capacity of leptin and prolactin receptors were both enhanced at a level comparable to that observed for the VEGF receptor. Thus, the combination of ZZ-BNC and Fc-fused receptors could significantly improve the function of ligand-binding assays. Copyright © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Bio-inspired polarized skylight navigation: a review
NASA Astrophysics Data System (ADS)
Zhang, Xi; Wan, Yongqin; Li, Lijing
2015-12-01
The idea of using skylight polarization in navigation is learned from animals such as desert ants and honeybees. Various research groups have been working on the development of novel navigation systems inspired by polarized skylight. The research of background in polarized skylight navigation is introduced, and basic principle of the insects navigation is expatiated. Then, the research progress status at home and abroad in skylight polarization pattern, three bio-inspired polarized skylight navigation sensors and polarized skylight navigation are reviewed. Finally, the research focuses in the field of polarized skylight navigation are analyzed. At the same time, the trend of development and prospect in the future are predicted. It is believed that the review is helpful to people understand polarized skylight navigation and polarized skylight navigation sensors.
Basement Membrane-Based Glucose Sensor Coatings Enhance Continuous Glucose Monitoring in Vivo
Klueh, Ulrike; Qiao, Yi; Czajkowski, Caroline; Ludzinska, Izabela; Antar, Omar; Kreutzer, Donald L.
2015-01-01
Background: Implantable glucose sensors demonstrate a rapid decline in function that is likely due to biofouling of the sensor. Previous efforts directed at overcoming this issue has generally focused on the use of synthetic polymer coatings, with little apparent effect in vivo, clearly a novel approach is required. We believe that the key to extending sensor life span in vivo is the development of biocompatible basement membrane (BM) based bio-hydrogels as coatings for glucose sensors. Method: BM based bio-hydrogel sensor coatings were developed using purified BM preparations (ie, Cultrex from Trevigen Inc). Modified Abbott sensors were coated with Cultrex BM extracts. Sensor performance was evaluated for the impact of these coatings in vitro and in vivo in a continuous glucose monitoring (CGM) mouse model. In vivo sensor function was assessed over a 28-day time period expressed as mean absolute relative difference (MARD) values. Tissue reactivity of both Cultrex coated and uncoated glucose sensors was evaluated at 7, 14, 21 and 28 days post–sensor implantation with standard histological techniques. Results: The data demonstrate that Cultrex-based sensor coatings had no effect on glucose sensor function in vitro. In vivo glucose sensor performance was enhanced following BM coating as determined by MARD analysis, particularly in weeks 2 and 3. In vivo studies also demonstrated that Cultrex coatings significantly decreased sensor-induced tissue reactions at the sensor implantation sites. Conclusion: Basement-membrane-based sensor coatings enhance glucose sensor function in vivo, by minimizing or preventing sensor-induced tissues reactions. PMID:26306494
Basement Membrane-Based Glucose Sensor Coatings Enhance Continuous Glucose Monitoring in Vivo.
Klueh, Ulrike; Qiao, Yi; Czajkowski, Caroline; Ludzinska, Izabela; Antar, Omar; Kreutzer, Donald L
2015-08-25
Implantable glucose sensors demonstrate a rapid decline in function that is likely due to biofouling of the sensor. Previous efforts directed at overcoming this issue has generally focused on the use of synthetic polymer coatings, with little apparent effect in vivo, clearly a novel approach is required. We believe that the key to extending sensor life span in vivo is the development of biocompatible basement membrane (BM) based bio-hydrogels as coatings for glucose sensors. BM based bio-hydrogel sensor coatings were developed using purified BM preparations (ie, Cultrex from Trevigen Inc). Modified Abbott sensors were coated with Cultrex BM extracts. Sensor performance was evaluated for the impact of these coatings in vitro and in vivo in a continuous glucose monitoring (CGM) mouse model. In vivo sensor function was assessed over a 28-day time period expressed as mean absolute relative difference (MARD) values. Tissue reactivity of both Cultrex coated and uncoated glucose sensors was evaluated at 7, 14, 21 and 28 days post-sensor implantation with standard histological techniques. The data demonstrate that Cultrex-based sensor coatings had no effect on glucose sensor function in vitro. In vivo glucose sensor performance was enhanced following BM coating as determined by MARD analysis, particularly in weeks 2 and 3. In vivo studies also demonstrated that Cultrex coatings significantly decreased sensor-induced tissue reactions at the sensor implantation sites. Basement-membrane-based sensor coatings enhance glucose sensor function in vivo, by minimizing or preventing sensor-induced tissues reactions. © 2015 Diabetes Technology Society.
Ultraviolet light emitting diodes and bio-aerosol sensing
NASA Astrophysics Data System (ADS)
Davitt, Kristina M.
Recent interest in compact ultraviolet (UV) light emitters has produced advances in material quality and device performance from aluminum-rich alloys of the nitride semiconductor system. The epitaxial growth of device structures from this material poses remarkable challenges, and state-of-the-art in semiconductor UV light sources at wavelengths shorter than 350 nm is currently limited to LEDs. A portion of the work presented in this thesis involves the design and characterization of UV LED structures, with particular focus on sub-300 nm LEDs which have only been demonstrated within the last four years. Emphasis has been placed on the integration of early devices with modest efficiencies and output powers into a practical, fluorescence-based bio-sensing instrument. The quality of AlGaInN and AlGaN-based materials is characterized by way of the performance of 340 nm and 290 nm LEDs respectively. A competitive level of device operation is achieved, although much room remains for improvement in the efficiency of light emission from this material system. A preliminary investigation of 300 nm LEDs grown on bulk AIN shows promising electrical and optical characteristics, and illustrates the numerous advantages that this native substrate offers to the epitaxy of wide bandgap nitride semiconductors. The application of UV LEDs to the field of bio-aerosol sensing is pursued by constructing an on-the-fly fluorescence detection system. A linear array of UV LEDs is designed and implemented, and the capability of test devices to excite native fluorescence from bacterial spores is established. In order to fully capitalize on the reduction in size afforded by LEDs, effort is invested in re-engineering the remaining sensor components. Operation of a prototype system for physically sorting bio-aerosols based on fluorescence spectra acquired in real-time from single airborne particles excited by a UV-LED array is demonstrated using the bio-fluorophores NADH and tryptophan. Sensor
A Review: Origins of the Dielectric Properties of Proteins and Potential Development as Bio-Sensors
Bibi, Fabien; Villain, Maud; Guillaume, Carole; Sorli, Brice; Gontard, Nathalie
2016-01-01
Polymers can be classified as synthetic polymers and natural polymers, and are often characterized by their most typical functions namely their high mechanical resistivity, electrical conductivity and dielectric properties. This bibliography report consists in: (i) Defining the origins of the dielectric properties of natural polymers by reviewing proteins. Despite their complex molecular chains, proteins present several points of interest, particularly, their charge content conferring their electrical and dielectric properties; (ii) Identifying factors influencing the dielectric properties of protein films. The effects of vapors and gases such as water vapor, oxygen, carbon dioxide, ammonia and ethanol on the dielectric properties are put forward; (iii) Finally, potential development of protein films as bio-sensors coated on electronic devices for detection of environmental changes particularly humidity or carbon dioxide content in relation with dielectric properties variations are discussed. As the study of the dielectric properties implies imposing an electric field to the material, it was necessary to evaluate the impact of frequency on the polymers and subsequently on their structure. Characterization techniques, on the one hand dielectric spectroscopy devoted for the determination of the glass transition temperature among others, and on the other hand other techniques such as infra-red spectroscopy for structure characterization as a function of moisture content for instance are also introduced. PMID:27527179
NASA Astrophysics Data System (ADS)
Burman, Jerry; Hespanha, Joao; Madhow, Upamanyu; Pham, Tien
2011-06-01
A team consisting of Teledyne Scientific Company, the University of California at Santa Barbara and the Army Research Laboratory* is developing technologies in support of automated data exfiltration from heterogeneous battlefield sensor networks to enhance situational awareness for dismounts and command echelons. Unmanned aerial vehicles (UAV) provide an effective means to autonomously collect data from a sparse network of unattended ground sensors (UGSs) that cannot communicate with each other. UAVs are used to reduce the system reaction time by generating autonomous collection routes that are data-driven. Bio-inspired techniques for search provide a novel strategy to detect, capture and fuse data. A fast and accurate method has been developed to localize an event by fusing data from a sparse number of UGSs. This technique uses a bio-inspired algorithm based on chemotaxis or the motion of bacteria seeking nutrients in their environment. A unique acoustic event classification algorithm was also developed based on using swarm optimization. Additional studies addressed the problem of routing multiple UAVs, optimally placing sensors in the field and locating the source of gunfire at helicopters. A field test was conducted in November of 2009 at Camp Roberts, CA. The field test results showed that a system controlled by bio-inspired software algorithms can autonomously detect and locate the source of an acoustic event with very high accuracy and visually verify the event. In nine independent test runs of a UAV, the system autonomously located the position of an explosion nine times with an average accuracy of 3 meters. The time required to perform source localization using the UAV was on the order of a few minutes based on UAV flight times. In June 2011, additional field tests of the system will be performed and will include multiple acoustic events, optimal sensor placement based on acoustic phenomenology and the use of the International Technology Alliance (ITA
NASA Technical Reports Server (NTRS)
Werdell, P. Jeremy; Fargion, Giulietta S. (Editor); McClain, Charles R. (Editor); Bailey, Sean W.
2002-01-01
Satellite ocean color missions require an abundance of high-quality in situ measurements for bio-optical and atmospheric algorithm development and post-launch product validation and sensor calibration. To facilitate the assembly of a global data set, the NASA Sea-viewing Wide Field-of-view (SeaWiFS) Project developed the Seafaring Bio-optical Archive and Storage System (SeaBASS), a local repository for in situ data regularly used in their scientific analyses. The system has since been expanded to contain data sets collected by the NASA Sensor Intercalibration and Merger for Biological and Interdisciplinary Oceanic Studies (SIMBIOS) Project, as part of NASA Research Announcements NRA-96-MTPE-04 and NRA-99-OES-99. SeaBASS is a well moderated and documented hive for bio-optical data with a simple, secure mechanism for locating and extracting data based on user inputs. Its holdings are available to the general public with the exception of the most recently collected data sets. Extensive quality assurance protocols, comprehensive data and system documentation, and the continuation of an archive and relational database management system (RDBMS) suitable for bio-optical data all contribute to the continued success of SeaBASS. This document provides an overview of the current operational SeaBASS system.
Biomedical sensor design using analog compressed sensing
NASA Astrophysics Data System (ADS)
Balouchestani, Mohammadreza; Krishnan, Sridhar
2015-05-01
The main drawback of current healthcare systems is the location-specific nature of the system due to the use of fixed/wired biomedical sensors. Since biomedical sensors are usually driven by a battery, power consumption is the most important factor determining the life of a biomedical sensor. They are also restricted by size, cost, and transmission capacity. Therefore, it is important to reduce the load of sampling by merging the sampling and compression steps to reduce the storage usage, transmission times, and power consumption in order to expand the current healthcare systems to Wireless Healthcare Systems (WHSs). In this work, we present an implementation of a low-power biomedical sensor using analog Compressed Sensing (CS) framework for sparse biomedical signals that addresses both the energy and telemetry bandwidth constraints of wearable and wireless Body-Area Networks (BANs). This architecture enables continuous data acquisition and compression of biomedical signals that are suitable for a variety of diagnostic and treatment purposes. At the transmitter side, an analog-CS framework is applied at the sensing step before Analog to Digital Converter (ADC) in order to generate the compressed version of the input analog bio-signal. At the receiver side, a reconstruction algorithm based on Restricted Isometry Property (RIP) condition is applied in order to reconstruct the original bio-signals form the compressed bio-signals with high probability and enough accuracy. We examine the proposed algorithm with healthy and neuropathy surface Electromyography (sEMG) signals. The proposed algorithm achieves a good level for Average Recognition Rate (ARR) at 93% and reconstruction accuracy at 98.9%. In addition, The proposed architecture reduces total computation time from 32 to 11.5 seconds at sampling-rate=29 % of Nyquist rate, Percentage Residual Difference (PRD)=26 %, Root Mean Squared Error (RMSE)=3 %.
Cennamo, Nunzio; D'Agostino, Girolamo; Donà, Alice; Dacarro, Giacomo; Pallavicini, Piersandro; Pesavento, Maria; Zeni, Luigi
2013-01-01
In this paper a refractive index sensor based on localized surface plasmon resonance (LSPR) in a Plastic Optical Fiber (POF), is presented and experimentally tested. LSPR is achieved exploiting five-branched gold nanostars (GNS) obtained using Triton X-100 in a seed-growth synthesis. They have the uncommon feature of three localized surface plasmon resonances. The strongest LSPRs fall in two ranges, one in the 600–900 nm range (LSPR 2) and the other one in the 1,100–1,600 nm range (LSPR 3), both sensible to refractive index changes. Anyway, due to the extremely strong attenuation (>102 dB/m) of the employed POF in the 1,100–1,600 nm range, only LSPR 2 will be exploited for refractive index change measurements, useful for bio-chemical sensing applications, as a proof of principle of the possibility of realizing a compact, low cost and easy-to-use GNS based device. PMID:24172284
Cennamo, Nunzio; D'Agostino, Girolamo; Donà, Alice; Dacarro, Giacomo; Pallavicini, Piersandro; Pesavento, Maria; Zeni, Luigi
2013-10-29
In this paper a refractive index sensor based on localized surface plasmon resonance (LSPR) in a Plastic Optical Fiber (POF), is presented and experimentally tested. LSPR is achieved exploiting five-branched gold nanostars (GNS) obtained using Triton X-100 in a seed-growth synthesis. They have the uncommon feature of three localized surface plasmon resonances. The strongest LSPRs fall in two ranges, one in the 600-900 nm range (LSPR 2) and the other one in the 1,100-1,600 nm range (LSPR 3), both sensible to refractive index changes. Anyway, due to the extremely strong attenuation (>10(2) dB/m) of the employed POF in the 1,100-1,600 nm range, only LSPR 2 will be exploited for refractive index change measurements, useful for bio-chemical sensing applications, as a proof of principle of the possibility of realizing a compact, low cost and easy-to-use GNS based device.
Magnetoelastic sensor for characterizing properties of thin-film/coatings
NASA Technical Reports Server (NTRS)
Bachas, Leonidas G. (Inventor); Barrett, Gary (Inventor); Grimes, Craig A. (Inventor); Kouzoudis, Dimitris (Inventor); Schmidt, Stefan (Inventor)
2004-01-01
An apparatus for determining elasticity characteristics of a thin-film layer. The apparatus comprises a sensor element having a base magnetostrictive element at least one surface of which is at least partially coated with the thin-film layer. The thin-film layer may be of a variety of materials (having a synthetic and/or bio-component) in a state or form capable of being deposited, manually or otherwise, on the base element surface, such as by way of eye-dropper, melting, dripping, brushing, sputtering, spraying, etching, evaporation, dip-coating, laminating, etc. Among suitable thin-film layers for the sensor element of the invention are fluent bio-substances, thin-film deposits used in manufacturing processes, polymeric coatings, paint, an adhesive, and so on. A receiver, preferably remotely located, is used to measure a plurality of values for magneto-elastic emission intensity of the sensor element in either characterization: (a) the measure of the plurality of values is used to identify a magneto-elastic resonant frequency value for the sensor element; and (b) the measure of the plurality of successive values is done at a preselected magneto-elastic frequency.
Cooperation among wirelessly connected static and mobile sensor nodes for surveillance applications.
de Freitas, Edison Pignaton; Heimfarth, Tales; Vinel, Alexey; Wagner, Flávio Rech; Pereira, Carlos Eduardo; Larsson, Tony
2013-09-25
This paper presents a bio-inspired networking strategy to support the cooperation between static sensors on the ground and mobile sensors in the air to perform surveillance missions in large areas. The goal of the proposal is to provide low overhead in the communication among sensor nodes, while allocating the mobile sensors to perform sensing activities requested by the static ones. Simulations have shown that the strategy is efficient in maintaining low overhead and achieving the desired coordination.
NASA Astrophysics Data System (ADS)
Bhalla, Suresh; Srivastava, Shashank; Suresh, Rupali; Moharana, Sumedha; Kaur, Naveet; Gupta, Ashok
2015-03-01
This paper presents a case for extension of structural health monitoring (SHM) technologies to offer solutions for biomedical problems. SHM research has made remarkable progress during the last two/ three decades. These technologies are now being extended for possible applications in the bio-medical field. Especially, smart materials, such as piezoelectric ceramic (PZT) patches and fibre-Bragg grating (FBG) sensors, offer a new set of possibilities to the bio-medical community to augment their conventional set of sensors, tools and equipment. The paper presents some of the recent extensions of SHM, such as condition monitoring of bones, monitoring of dental implant post surgery and foot pressure measurement. Latest developments, such as non-bonded configuration of PZT patches for monitoring bones and possible applications in osteoporosis detection, are also discussed. In essence, there is a whole new gamut of new possibilities for SHM technologies making their foray into the bi-medical sector.
Abushagur, Abdulfatah A.G.; Arsad, Norhana; Ibne Reaz, Mamun; Ashrif, A.; Bakar, A.
2014-01-01
The large interest in utilising fibre Bragg grating (FBG) strain sensors for minimally invasive surgery (MIS) applications to replace conventional electrical tactile sensors has grown in the past few years. FBG strain sensors offer the advantages of optical fibre sensors, such as high sensitivity, immunity to electromagnetic noise, electrical passivity and chemical inertness, but are not limited by phase discontinuity or intensity fluctuations. FBG sensors feature a wavelength-encoding sensing signal that enables distributed sensing that utilises fewer connections. In addition, their flexibility and lightness allow easy insertion into needles and catheters, thus enabling localised measurements inside tissues and blood. Two types of FBG tactile sensors have been emphasised in the literature: single-point and array FBG tactile sensors. This paper describes the current design, development and research of the optical fibre tactile techniques that are based on FBGs to enhance the performance of MIS procedures in general. Providing MIS or microsurgery surgeons with accurate and precise measurements and control of the contact forces during tissues manipulation will benefit both surgeons and patients. PMID:24721774
Bio-prospecting of distillery yeasts as bio-control and bio-remediation agents.
Ubeda, Juan F; Maldonado, María; Briones, Ana I; Francisco, J Fernández; González, Francisco J
2014-05-01
This work constitutes a preliminary study in which the capacity of non-Saccharomyces yeasts isolated from ancient distilleries as bio-control agents against moulds and in the treatment of waste waters contaminated by heavy metals-i.e. bio-remediation-is shown. In the first control assays, antagonist effect between non-Saccharomyces yeasts, their extracts and supernatants against some moulds, analysing the plausible (not exhaustive) involved factors were qualitatively verified. In addition, two enzymatic degrading properties of cell wall plant polymers, quitinolitic and pectinolitic, were screened. Finally, their use as agents of bio-remediation of three heavy metals (cadmium, chromium and lead) was analysed semi-quantitatively. The results showed that all isolates belonging to Pichia species effectively inhibited all moulds assayed. Moreover, P. kudriavzevii is a good candidate for both bio-control and bio-remediation because it inhibited moulds and accumulated the major proportion of the three tested metals.
Capacitance Sensors for Nondestructive Moisture Determination in Agricultural and Bio-fuel materials
USDA-ARS?s Scientific Manuscript database
Moisture content of wood chips, pellets, switch grass powders, and similar organic bio-fuel materials is an important property to be known to determine their utility and energy efficiency at various stages of their processing and storage. Several moisture measuring instruments are available in the m...
The Bermuda Bio-Optics Program (BBOP). Chapter 16
NASA Technical Reports Server (NTRS)
Siegel, David A.
2001-01-01
The Bermuda Bio-Optics Project (BBOP) is a collaborative effort between the Institute for Computational Earth System Science (ICESS) at the University of California at Santa Barbara (UCSB) and the Bermuda Biological Station for Research (BBSR). This research program is designed to characterize light availability and utilization in the Sargasso Sea, and to provide an optical link by which biogeochemical observations may be used to evaluate bio-optical models for pigment concentration, primary production, and sinking particle fluxes from satellite-based ocean color sensors. The BBOP time-series was initiated in 1992, and is carried out in conjunction with the US JGOFS Bermuda Atlantic Time-series Study (BATS) at the Bermuda Biological Station for Research. The BATS program itself has been observing biogeochemical processes (primary productivity, particle flux at and elemental cycles) in the mesotrophic waters of the Sargasso Sea since 1988. Closely affiliated with BBOP and BATS is a separate NASA-funded study of the spatial variability of biogeochemical processes in the Sargasso Sea using high-resolution Advanced Very High Resolution Radiometer (AVHRR) and Sea-Viewing Wide Field-of-view Sensor (SeaWiFS) data collected at Bermuda. The collaboration between BATS and BBOP measurements has resulted in a unique data set that addresses not only the SIMBIOS goals but also the broader issues of important factors controlling the carbon cycle.
Mimosa-inspired design of a flexible pressure sensor with touch sensitivity.
Su, Bin; Gong, Shu; Ma, Zheng; Yap, Lim Wei; Cheng, Wenlong
2015-04-24
A bio-inspired flexible pressure sensor is generated with high sensitivity (50.17 kPa(-1)), quick responding time (<20 ms), and durable stability (negligible loading-unloading signal changes over 10 000 cycles). Notably, the key resource of surface microstructures upon sensor substrates results from the direct molding of natural mimosa leaves, presenting a simple, environment-friendly and easy scale-up fabrication process for these flexible pressure sensors. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
A Raman spectroscopy bio-sensor for tissue discrimination in surgical robotics.
Ashok, Praveen C; Giardini, Mario E; Dholakia, Kishan; Sibbett, Wilson
2014-01-01
We report the development of a fiber-based Raman sensor to be used in tumour margin identification during endoluminal robotic surgery. Although this is a generic platform, the sensor we describe was adapted for the ARAKNES (Array of Robots Augmenting the KiNematics of Endoluminal Surgery) robotic platform. On such a platform, the Raman sensor is intended to identify ambiguous tissue margins during robot-assisted surgeries. To maintain sterility of the probe during surgical intervention, a disposable sleeve was specially designed. A straightforward user-compatible interface was implemented where a supervised multivariate classification algorithm was used to classify different tissue types based on specific Raman fingerprints so that it could be used without prior knowledge of spectroscopic data analysis. The protocol avoids inter-patient variability in data and the sensor system is not restricted for use in the classification of a particular tissue type. Representative tissue classification assessments were performed using this system on excised tissue. Copyright © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
BioRadioTransmitter: a self-powered wireless glucose-sensing system.
Hanashi, Takuya; Yamazaki, Tomohiko; Tsugawa, Wakako; Ikebukuro, Kazunori; Sode, Koji
2011-09-01
Although an enzyme fuel cell can be utilized as a glucose sensor, the output power generated is too low to power a device such as a currently available transmitter and operating system, and an external power source is required for operating an enzyme-fuel-cell-based biosensing system. We proposed a novel biosensor that we named BioCapacitor, in which a capacitor serves as a transducer. In this study, we constructed a new BioCapacitor-based system with an added radio-transmitter circuit and a miniaturized enzyme fuel cell. A miniaturized direct-electron-transfer-type compartmentless enzyme fuel cell was constructed with flavin adenine dinucleotide-dependent glucose dehydrogenase complex-based anode and a bilirubin-oxidase-based cathode. For construction of a BioRadioTransmitter wireless sensing system, a capacitor, an ultra-low-voltage charge-pump-integrated circuit, and Hartley oscillator circuit were connected to the miniaturized enzyme fuel cell. A radio-receiver circuit, comprising two field-effect transistors and a coil as an antenna, was used to amplify the signal generated from the biofuel cells. Radio wave signals generated by the BioRadioTransmitter were received, amplified, and converted from alternate to direct current by the radio receiver. When the capacitor discharges in the presence of glucose, the BioRadioTransmitter generates a radio wave, which is monitored by a radio receiver connected wirelessly to the sensing device. Magnitude of the radio wave transmission frequency change observed at the radio receiver was correlated to glucose concentration in the fuel cells. We constructed a stand-alone, self-powered, wireless glucose-sensing system called a BioRadioTransmitter by using a radio transmitter in which the radio wave transmission frequency changes with the glucose concentration in the fuel cell. The BioRadioTransmitter is a significant advance toward construction of an implantable continuous glucose monitor. © 2011 Diabetes Technology Society.
Resistive flex sensors: a survey
NASA Astrophysics Data System (ADS)
Saggio, Giovanni; Riillo, Francesco; Sbernini, Laura; Quitadamo, Lucia Rita
2016-01-01
Resistive flex sensors can be used to measure bending or flexing with relatively little effort and a relatively low budget. Their lightness, compactness, robustness, measurement effectiveness and low power consumption make these sensors useful for manifold applications in diverse fields. Here, we provide a comprehensive survey of resistive flex sensors, taking into account their working principles, manufacturing aspects, electrical characteristics and equivalent models, useful front-end conditioning circuitry, and physic-bio-chemical aspects. Particular effort is devoted to reporting on and analyzing several applications of resistive flex sensors, related to the measurement of body position and motion, and to the implementation of artificial devices. In relation to the human body, we consider the utilization of resistive flex sensors for the measurement of physical activity and for the development of interaction/interface devices driven by human gestures. Concerning artificial devices, we deal with applications related to the automotive field, robots, orthosis and prosthesis, musical instruments and measuring tools. The presented literature is collected from different sources, including bibliographic databases, company press releases, patents, master’s theses and PhD theses.
Bio-implantable passive on-chip RF-MEMS strain sensing resonators for orthopaedic applications
NASA Astrophysics Data System (ADS)
Melik, Rohat; Kosku Perkgoz, Nihan; Unal, Emre; Puttlitz, Christian; Demir, Hilmi Volkan
2008-11-01
One out of ten bone fractures does not heal properly due to improper load distribution and strain profiles during the healing process. To provide implantable tools for the assessment of bone fractures, we have designed novel, bio-implantable, passive, on-chip, RF-MEMS strain sensors that rely on the resonance frequency shift with mechanical deformation. For this purpose, we modeled, fabricated and experimentally characterized two on-chip sensors with high quality factors for in vivo implantation. One of the sensors has an area of ~0.12 mm2 with a quality factor of ~60 and the other has an area of ~0.07 mm2 with a quality factor of ~70. To monitor the mechanical deformation by measuring the change in the resonance frequencies with the applied load, we employed a controllable, point load applying experimental setup designed and constructed for in vitro characterization. In the case of the sensor with the larger area, when we apply a load of 3920 N, we obtain a frequency shift of ~330 MHz and a quality factor of ~76. For the smaller sensor, the frequency shift and the quality factor are increased to 360 MHz and 95, respectively. These data demonstrate that our sensor chips have the capacity to withstand relatively high physiologic loads, and that the concomitant and very large resonant frequency shift with the applied load is achieved while maintaining a high signal quality factor. These experiments demonstrate that these novel sensors have the capacity for producing high sensitivity strain readout, even when the total device area is considerably small. Also, we have demonstrated that our bio-implantable, passive sensors deliver a telemetric, real-time readout of the strain on a chip. Placing two more resonators on the sides of the sensor to serve as transmitter and receiver antennas, we achieved to transfer contactless power and read out loads in the absence of direct wiring to the sensor. With this model, where telemetric measurements become simpler due to the fact
Open Loop Structure Low Cost Integrated Differential Inductive Micro Magnetic Volumetric Bio-Sensors
NASA Astrophysics Data System (ADS)
Khodadadi, Mohammad; Chang, Long; Litvinov, Dimitri
This investigation proposes a study, model, simulate and experiment innovative very low cost Magnetic induction biosensor for point of care diagnostics. The biosensor consists of 2 ``semi-loops'' in a micro fluidic channel, one as a sensor and one as a reference, the design takes advantage of microfabrication processes to produce more precise structures to improve sensitivity. Besides the attractively low cost, this biosensor has many advantages. Since the detector is basically a shaped wire, it is inherently robust and reliable. Typical errors in fabricating the wires will not affect its performance and it is sensing volumetric, unlike GMR-based sensors used in biosensor systems that boast single particle detection. Due to small dimensions the sensors do not need to be calibrated. This sensor also has a large range of detection since its sensitivity is proportional to the excitation frequency. Being able to sense Magnetic nano particles in the volume is an advantage in term of trapping MNPs and sensitivity and functionality. Basically, this new brilliant design, fill the gap between the fabricated sensors and hand wounded sensors.
Bio-inspired nano-sensor-enhanced CNN visual computer.
Porod, Wolfgang; Werblin, Frank; Chua, Leon O; Roska, Tamas; Rodriguez-Vazquez, Angel; Roska, Botond; Fay, Patrick; Bernstein, Gary H; Huang, Yih-Fang; Csurgay, Arpad I
2004-05-01
Nanotechnology opens new ways to utilize recent discoveries in biological image processing by translating the underlying functional concepts into the design of CNN (cellular neural/nonlinear network)-based systems incorporating nanoelectronic devices. There is a natural intersection joining studies of retinal processing, spatio-temporal nonlinear dynamics embodied in CNN, and the possibility of miniaturizing the technology through nanotechnology. This intersection serves as the springboard for our multidisciplinary project. Biological feature and motion detectors map directly into the spatio-temporal dynamics of CNN for target recognition, image stabilization, and tracking. The neural interactions underlying color processing will drive the development of nanoscale multispectral sensor arrays for image fusion. Implementing such nanoscale sensors on a CNN platform will allow the implementation of device feedback control, a hallmark of biological sensory systems. These biologically inspired CNN subroutines are incorporated into the new world of analog-and-logic algorithms and software, containing also many other active-wave computing mechanisms, including nature-inspired (physics and chemistry) as well as PDE-based sophisticated spatio-temporal algorithms. Our goal is to design and develop several miniature prototype devices for target detection, navigation, tracking, and robotics. This paper presents an example illustrating the synergies emerging from the convergence of nanotechnology, biotechnology, and information and cognitive science.
Bio-inspired multi-mode optic flow sensors for micro air vehicles
NASA Astrophysics Data System (ADS)
Park, Seokjun; Choi, Jaehyuk; Cho, Jihyun; Yoon, Euisik
2013-06-01
Monitoring wide-field surrounding information is essential for vision-based autonomous navigation in micro-air-vehicles (MAV). Our image-cube (iCube) module, which consists of multiple sensors that are facing different angles in 3-D space, can be applied to the wide-field of view optic flows estimation (μ-Compound eyes) and to attitude control (μ- Ocelli) in the Micro Autonomous Systems and Technology (MAST) platforms. In this paper, we report an analog/digital (A/D) mixed-mode optic-flow sensor, which generates both optic flows and normal images in different modes for μ- Compound eyes and μ-Ocelli applications. The sensor employs a time-stamp based optic flow algorithm which is modified from the conventional EMD (Elementary Motion Detector) algorithm to give an optimum partitioning of hardware blocks in analog and digital domains as well as adequate allocation of pixel-level, column-parallel, and chip-level signal processing. Temporal filtering, which may require huge hardware resources if implemented in digital domain, is remained in a pixel-level analog processing unit. The rest of the blocks, including feature detection and timestamp latching, are implemented using digital circuits in a column-parallel processing unit. Finally, time-stamp information is decoded into velocity from look-up tables, multiplications, and simple subtraction circuits in a chip-level processing unit, thus significantly reducing core digital processing power consumption. In the normal image mode, the sensor generates 8-b digital images using single slope ADCs in the column unit. In the optic flow mode, the sensor estimates 8-b 1-D optic flows from the integrated mixed-mode algorithm core and 2-D optic flows with an external timestamp processing, respectively.
Christodoulides, Nicolaos J.; McRae, Michael P.; Abram, Timothy J.; Simmons, Glennon W.; McDevitt, John T.
2017-01-01
The lack of standard tools and methodologies and the absence of a streamlined multimarker approval process have hindered the translation rate of new biomarkers into clinical practice for a variety of diseases afflicting humankind. Advanced novel technologies with superior analytical performance and reduced reagent costs, like the programmable bio-nano-chip system featured in this article, have potential to change the delivery of healthcare. This universal platform system has the capacity to digitize biology, resulting in a sensor modality with a capacity to learn. With well-planned device design, development, and distribution plans, there is an opportunity to translate benchtop discoveries in the genomics, proteomics, metabolomics, and glycomics fields by transforming the information content of key biomarkers into actionable signatures that can empower physicians and patients for a better management of healthcare. While the process is complicated and will take some time, showcased here are three application areas for this flexible platform that combines biomarker content with minimally invasive or non-invasive sampling, such as brush biopsy for oral cancer risk assessment; serum, plasma, and small volumes of blood for the assessment of cardiac risk and wellness; and oral fluid sampling for drugs of abuse testing at the point of need. PMID:28589118
Disposable soft 3 axis force sensor for biomedical applications.
Chathuranga, Damith Suresh; Zhongkui Wang; Yohan Noh; Nanayakkara, Thrishantha; Hirai, Shinichi
2015-08-01
This paper proposes a new disposable soft 3D force sensor that can be used to calculate either force or displacement and vibrations. It uses three Hall Effect sensors orthogonally placed around a cylindrical beam made of silicon rubber. A niobium permanent magnet is inside the silicon. When a force is applied to the end of the cylinder, it is compressed and bent to the opposite side of the force displacing the magnet. This displacement causes change in the magnetic flux around the ratiomatric linear sensors (Hall Effect sensors). By analysing these changes, we calculate the force or displacement in three directions using a lookup table. This sensor can be used in minimal invasive surgery and haptic feedback applications. The cheap construction, bio-compatibility and ease of miniaturization are few advantages of this sensor. The sensor design, and its characterization are presented in this work.
Experiment study of bio-tissue's temperature irradiated by laser based on optical fiber F-P sensor
NASA Astrophysics Data System (ADS)
Shan, Ning; Liu, Xia
2014-08-01
Laser has several advantages, such as strong anti-interference ability, quick speed, high power, agility and precision. It is widely applied in military and medicine fields. When laser acts on human body, biological tissue of human body will appear the phenomenon of ablation and carbonization and solidification. In order to effectively defend excess damage by laser, the thermal effect research of skin tissue should be carried out. Temperature is a key parameter in the processing between laser and bio-tissue. It is the mostly foundation using analyze size of thermal damage area and forecast thermal damage degree. In this paper, the low fineness optical fiber F-P sensing system for temperature measurement is designed and established. The real-time measurement system of temperature generated by laser irradiating bio-tissue is build based on the sensing system. The temperature distributing generated by laser in the bio-tissue is studied through experiment when the spot diameter of emission laser is difference with the same energy density and the energy density is difference with the same spot diameter of emission laser. The experimental results show that the sensing system can be used to the real-time temperature measurement of bio-tissue efficiency. It has small bulk. Its outer diameter is 250μm. And the hurt for bio-tissue is small. It has high respond speed. The respond time of temperature is less than 1s. These can be satisfied with practice demand. When the energy density of laser is same, the temperature rising in the same location is low along the spot diameter of emission laser increasing. When the spot diameter of emission laser is same, the temperature rising in the same location is increasing along with the energy density of laser increasing.
[Progress in bio-based polyamides].
Huang, Zhengqiang; Cui, Zhe; Zhang, Heming; Fu, Peng; Zhao, Qingxiang; Liu, Minying
2016-06-25
Bio-based polyamides are environment-friendly polymers. The precursors of bio-based polyamides come from bio-based materials such as castor oil, glucose and animal oil. Bio-based polyamides precursors include bio-based amino acids, bio-based lactams, bio-based diprotic acid and bio-based diamines. In this paper, we discussed the route of the precursors of bio-based polyamides that come from bio-based materials. We discussed the properties of bio-based polyamides. Bio-based PA11and bio-based PA1010 are well-known bio-based polyamides; we discussed the origin materials of the precursors, the route of manufacturing bio-based PA11 and PA1010, and their modifications status. The variety, classification and commercial production of bio-based polyamides were described in details, as well as bio-based polyamides development in China.
Ma'mun, Ahmed; Abd El-Rahman, Mohamed K; Abd El-Kawy, Mohamed
2018-05-30
In recent years, the whole field of ion-selective electrodes(ISEs) in pharmaceutical sciences has expanded far beyond its original roots. The diverse range of opportunities offered by ISEs was broadly used in a number of pharmaceutical applications, with topics presented ranging from bioanalysis of drugs and metabolites, to protein binding studies, green analytical chemistry, impurity profiling, and drug dissolution in biorelevant media. Inspired from these advances and with the aim of extending the functional capabilities of ISEs, the primary focus of the present paper is the utilization of ISE as a tool in personalized medicine. Given the opportunity to explore biological events in real-time (such as drug metabolism) could be central to personalized medicine. (ATR) is a chemo-degradable and bio-degradable pharmaceutically active drug. Laudanosine (LDS) is the major degradation product and metabolite of ATR and is potentially toxic and reported to possess epileptogenic activity which increases the risk of convulsive effects. In this work, ATR have been subjected to both chemical and biological hydrolysis, and the course of the reactions is monitored by means of a ISE. In this study, we have designed an efficient real-time tracking strategy which substantially resolve the challenges of the ATR chemical and biological degradation kinetics. By utilizing a potentiometric sensor, tracking of ATR chemical and biological degradation kinetics can be performed in a very short time with excellent accuracy. The LOD was calculated to be 0.23 μmol L -1 , the potential drift was investigated over a period of 60 min and the value was 0.25 mV h -1 . Real serum samples for measurement the rate of in vitro metabolism of ATR was performed. Furthermore, a full description of the fabricated screen-printed sensor was presented. Copyright © 2018 Elsevier B.V. All rights reserved.
NASA Astrophysics Data System (ADS)
Chen, Yi; Liu, Logan
2014-03-01
The interaction of biomolecules and solid-state nanomaterials at the nano-bio interfaces is a long-lasting research topic in nanotechnology. Historically, fundamental problems, such as the electron transfer, energy transfer, and plasmonic interaction at the bio-nano interfaces, have been intensively studied, and revolutionary technologies, such as molecular electronics, peptide chips, nanoplasmonic sensors, have been created. With the combined effort of molecular dynamics simulation and surface-enhanced Raman spectroscopy, we studied the external electric field-induced conformation changes of dodecapeptide probes tethered to a nanostructured metallic surface. Through this study, we demonstrated a reversible manipulation of the biomolecule conformations as well as an in situ eletro-optical detection of the subnanometer conformational changes at the bio-nano interfaces. Based on the proof-of-concept established in this study, we further propose a novel nanophotonic peptide phosphorylation sensor for high-sensitive peptide kinase profiling. We have also demonstrated the same SERS nano-bio-chip can be used for environmental monitoring applications, such as detection of contaminants in drinking water at ultralow concentrates. The fabrication of this nanosensor is based on a single step, lithography-less nanomanufacturing process, which can produce hundreds of these chips in several minutes with nearly 100% yield and uniformity. Therefore, the demonstrated research can be readily translated into industrial mass productions.
Piezoelectric cantilever sensors
NASA Technical Reports Server (NTRS)
Shih, Wan Y. (Inventor); Shih, Wei-Heng (Inventor); Shen, Zuyan (Inventor)
2008-01-01
A piezoelectric cantilever with a non-piezoelectric, or piezoelectric tip useful as mass and viscosity sensors. The change in the cantilever mass can be accurately quantified by monitoring a resonance frequency shift of the cantilever. For bio-detection, antibodies or other specific receptors of target antigens may be immobilized on the cantilever surface, preferably on the non-piezoelectric tip. For chemical detection, high surface-area selective absorbent materials are coated on the cantilever tip. Binding of the target antigens or analytes to the cantilever surface increases the cantilever mass. Detection of target antigens or analytes is achieved by monitoring the cantilever's resonance frequency and determining the resonance frequency shift that is due to the mass of the adsorbed target antigens on the cantilever surface. The use of a piezoelectric unimorph cantilever allows both electrical actuation and electrical sensing. Incorporating a non-piezoelectric tip (14) enhances the sensitivity of the sensor. In addition, the piezoelectric cantilever can withstand damping in highly viscous liquids and can be used as a viscosity sensor in wide viscosity range.
NASA Astrophysics Data System (ADS)
Pignaton de Freitas, Edison; Heimfarth, Tales; Pereira, Carlos Eduardo; Morado Ferreira, Armando; Rech Wagner, Flávio; Larsson, Tony
2010-04-01
A current trend that is gaining strength in the wireless sensor network area is the use of heterogeneous sensor nodes in one coordinated overall network, needed to fulfill the requirements of sophisticated emerging applications, such as area surveillance systems. One of the main concerns when developing such sensor networks is how to provide coordination among the heterogeneous nodes, in order to enable them to efficiently respond the user needs. This study presents an investigation of strategies to coordinate a set of static sensor nodes on the ground cooperating with wirelessly connected Unmanned Aerial Vehicles (UAVs) carrying a variety of sensors, in order to provide efficient surveillance over an area of interest. The sensor nodes on the ground are set to issue alarms on the occurrence of a given event of interest, e.g. entrance of a non-authorized vehicle in the area, while the UAVs receive the issued alarms and have to decide which of them is the most suitable to handle the issued alarm. A bio-inspired coordination strategy based on the concept of pheromones is presented. As a complement of this strategy, a utility-based decision making approach is proposed.
Edison, Thomas Nesakumar Jebakumar Immanuel; Atchudan, Raji; Shim, Jae-Jin; Kalimuthu, Senthilkumar; Ahn, Byeong-Cheol; Lee, Yong Rok
2016-05-01
This paper reports turn-off fluorescence sensor for Fe(3+) ion in water using fluorescent N-doped carbon dots as a probe. A simple and efficient hydrothermal carbonization of Prunus avium fruit extract for the synthesis of fluorescent nitrogen-doped carbon dots (N-CDs) is described. This green approach proceeds quickly and provides good quality N-CDs. The mean size of synthesized N-CDs was approximately 7nm calculated from the high-resolution transmission electron microscopic images. X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy revealed the presence of -OH, -NH2, -COOH, and -CO functional groups over the surface of CDs. The N-CDs showed excellent fluorescent properties, and emitted blue fluorescence at 411nm upon excitation at 310nm. The calculated quantum yield of the synthesized N-CDs is 13% against quinine sulfate as a reference fluorophore. The synthesized N-CDs were used as a fluorescent probe towards the selective and sensitive detection of biologically important Fe(3+) ions in water by fluorescence spectroscopy and for bio-imaging of MDA-MB-231 cells. The limit of detection (LOD) and the Stern-Volmer quenching constant for the synthesized N-CDs were 0.96μM and 2.0958×10(3)M of Fe(3+) ions. The green synthesized N-CDs are efficiently used as a promising candidate for the detection of Fe(3+) ions and bio-imaging. Copyright © 2016 Elsevier B.V. All rights reserved.
Cognitive bio-radar: The natural evolution of bio-signals measurement.
Malafaia, Daniel; Oliveira, Beatriz; Ferreira, Pedro; Varum, Tiago; Vieira, José; Tomé, Ana
2016-10-01
In this article we discuss a novel approach to Bio-Radar, contactless measurement of bio-signals, called Cognitive Bio-Radar. This new approach implements the Bio-Radar in a Software Defined Radio (SDR) platform in order to obtain awareness of the environment where it operates. Due to this, the Cognitive Bio-Radar can adapt to its surroundings in order to have an intelligent usage of the radio frequency spectrum to improve its performance. In order to study the feasibility of such implementation, a SDR based Bio-Radar testbench was developed and evaluated. The prototype is shown to be able to acquire the heartbeat activity and the respiratory effort. The acquired data is compared with the acquisitions from a Biopac research data acquisition system, showing coherent results for both heartbeat and breathing rate.
Bio-inspired optical rotation sensor
NASA Astrophysics Data System (ADS)
O'Carroll, David C.; Shoemaker, Patrick A.; Brinkworth, Russell S. A.
2007-01-01
Traditional approaches to calculating self-motion from visual information in artificial devices have generally relied on object identification and/or correlation of image sections between successive frames. Such calculations are computationally expensive and real-time digital implementation requires powerful processors. In contrast flies arrive at essentially the same outcome, the estimation of self-motion, in a much smaller package using vastly less power. Despite the potential advantages and a few notable successes, few neuromorphic analog VLSI devices based on biological vision have been employed in practical applications to date. This paper describes a hardware implementation in aVLSI of our recently developed adaptive model for motion detection. The chip integrates motion over a linear array of local motion processors to give a single voltage output. Although the device lacks on-chip photodetectors, it includes bias circuits to use currents from external photodiodes, and we have integrated it with a ring-array of 40 photodiodes to form a visual rotation sensor. The ring configuration reduces pattern noise and combined with the pixel-wise adaptive characteristic of the underlying circuitry, permits a robust output that is proportional to image rotational velocity over a large range of speeds, and is largely independent of either mean luminance or the spatial structure of the image viewed. In principle, such devices could be used as an element of a velocity-based servo to replace or augment inertial guidance systems in applications such as mUAVs.
Smart Sensors: Why and when the origin was and why and where the future will be
NASA Astrophysics Data System (ADS)
Corsi, C.
2013-12-01
Smart Sensors is a technique developed in the 70's when the processing capabilities, based on readout integrated with signal processing, was still far from the complexity needed in advanced IR surveillance and warning systems, because of the enormous amount of noise/unwanted signals emitted by operating scenario especially in military applications. The Smart Sensors technology was kept restricted within a close military environment exploding in applications and performances in the 90's years thanks to the impressive improvements in the integrated signal read-out and processing achieved by CCD-CMOS technologies in FPA. In fact the rapid advances of "very large scale integration" (VLSI) processor technology and mosaic EO detector array technology allowed to develop new generations of Smart Sensors with much improved signal processing by integrating microcomputers and other VLSI signal processors. inside the sensor structure achieving some basic functions of living eyes (dynamic stare, non-uniformity compensation, spatial and temporal filtering). New and future technologies (Nanotechnology, Bio-Organic Electronics, Bio-Computing) are lightning a new generation of Smart Sensors extending the Smartness from the Space-Time Domain to Spectroscopic Functional Multi-Domain Signal Processing. History and future forecasting of Smart Sensors will be reported.
Detection of VX Simulants Using Piezoresistive Microcantilever Sensors
DOE Office of Scientific and Technical Information (OSTI.GOV)
Porter, Timothy L.; Venedam, Richard J.; Kyle, Kyle
2011-05-28
Piezoresistive microcantilever sensors may be used in a variety of sensing applications, including chemical analytes and some types of biological species. These sensors employ a tiny piezoresistive microcantilever functionalized with a “sensing material” that acts as a probe for the desired analyte. In this study, the microcantilever was partially embedded into the sensing material, producing a sensor element that is highly rigid and resistant to shock, making it suitable for portable or handheld operation. The sensing material matrix used was Hypol, a hydrogel capable of preserving the bio-functionality of molecules embedded into it. This matrix was combined with acetylcholinesterase tomore » form the finished sensing material. Results of exposing these sensors to a VX simulant, malathion, are presented for both vapor and liquid environments.« less
BIOS Security Analysis and a Kind of Trusted BIOS
NASA Astrophysics Data System (ADS)
Zhou, Zhenliu; Xu, Rongsheng
The BIOS's security threats to computer system are analyzed and security requirements for firmware BIOS are summarized in this paper. Through discussion about TCG's trust transitivity, a new approach about CRTM implementation based on BIOS is developed. In this paper, we also put forward a new trusted BIOS architecture-UTBIOS which is built on Intel Framework for EFI/UEFI. The trustworthiness of UTBIOS is based on trusted hardware TPM. In UTBIOS, trust encapsulation and trust measurement are used to construct pre-OS trust chain. Performance of trust measurement is also analyzed in the end.
Optical Graphene Gas Sensors Based on Microfibers: A Review
Wu, Yu; Yao, Baicheng; Yu, Caibin; Rao, Yunjiang
2018-01-01
Graphene has become a bridge across optoelectronics, mechanics, and bio-chemical sensing due to its unique photoelectric characteristics. Moreover, benefiting from its two-dimensional nature, this atomically thick film with full flexibility has been widely incorporated with optical waveguides such as fibers, realizing novel photonic devices including polarizers, lasers, and sensors. Among the graphene-based optical devices, sensor is one of the most important branch, especially for gas sensing, as rapid progress has been made in both sensing structures and devices in recent years. This article presents a comprehensive and systematic overview of graphene-based microfiber gas sensors regarding many aspects including sensing principles, properties, fabrication, interrogating and implementations. PMID:29565314
Bio-Inspired Polarized Skylight-Based Navigation Sensors: A Review
Karman, Salmah B.; Diah, S. Zaleha M.; Gebeshuber, Ille C.
2012-01-01
Animal senses cover a broad range of signal types and signal bandwidths and have inspired various sensors and bioinstrumentation devices for biological and medical applications. Insects, such as desert ants and honeybees, for example, utilize polarized skylight pattern-based information in their navigation activities. They reliably return to their nests and hives from places many kilometers away. The insect navigation system involves the dorsal rim area in their compound eyes and the corresponding polarization sensitive neurons in the brain. The dorsal rim area is equipped with photoreceptors, which have orthogonally arranged small hair-like structures termed microvilli. These are the specialized sensors for the detection of polarized skylight patterns (e-vector orientation). Various research groups have been working on the development of novel navigation systems inspired by polarized skylight-based navigation in animals. Their major contributions are critically reviewed. One focus of current research activities is on imitating the integration path mechanism in desert ants. The potential for simple, high performance miniaturized bioinstrumentation that can assist people in navigation will be explored. PMID:23202158
Bio-inspired polarized skylight-based navigation sensors: a review.
Karman, Salmah B; Diah, S Zaleha M; Gebeshuber, Ille C
2012-10-24
Animal senses cover a broad range of signal types and signal bandwidths and have inspired various sensors and bioinstrumentation devices for biological and medical applications. Insects, such as desert ants and honeybees, for example, utilize polarized skylight pattern-based information in their navigation activities. They reliably return to their nests and hives from places many kilometers away. The insect navigation system involves the dorsal rim area in their compound eyes and the corresponding polarization sensitive neurons in the brain. The dorsal rim area is equipped with photoreceptors, which have orthogonally arranged small hair-like structures termed microvilli. These are the specialized sensors for the detection of polarized skylight patterns (e-vector orientation). Various research groups have been working on the development of novel navigation systems inspired by polarized skylight-based navigation in animals. Their major contributions are critically reviewed. One focus of current research activities is on imitating the integration path mechanism in desert ants. The potential for simple, high performance miniaturized bioinstrumentation that can assist people in navigation will be explored.
NASA Technical Reports Server (NTRS)
Brown, Christopher W.; Subramaniam, Ajit; Culver, Mary; Brock, John C.
2001-01-01
Monitoring the health of US coastal waters is an important goal of the National Oceanic and Atmospheric Administration (NOAA). Satellite sensors are capable of providing daily synoptic data of large expanses of the US coast. Ocean color sensors, in particular, can be used to monitor the water quality of coastal waters on an operational basis. To appraise the validity of satellite-derived measurements, such as chlorophyll concentration, the bio-optical algorithms used to derive them must be evaluated in coastal environments. Towards this purpose, over 21 cruises in diverse US coastal waters have been conducted. Of these 21 cruises, 12 have been performed in conjunction with and under the auspices of the NASA/Sensor Intercomparison and Merger for Biological and Interdisciplinary Oceanic Studies (SIMBIOS) Project. The primary goal of these cruises has been to obtain in-situ measurements of downwelling irradiance, upwelling radiance, and chlorophyll concentrations in order to evaluate bio-optical algorithms that estimate chlorophyll concentration. In this Technical Memorandum, we evaluate the ability of five bio-optical algorithms, including the current Sea-Viewing Wide Field-of-view Sensor (SeaWiFS) algorithm, to estimate chlorophyll concentration in surface waters of the South Atlantic Bight (SAB). The SAB consists of a variety of environments including coastal and continental shelf regimes, Gulf Stream waters, and the Sargasso Sea. The biological and optical characteristics of the region is complicated by temporal and spatial variability in phytoplankton composition, primary productivity, and the concentrations of colored dissolved organic matter (CDOM) and suspended sediment. As such, the SAB is an ideal location to test the robustness of algorithms for coastal use.
NASA Astrophysics Data System (ADS)
Atamanchuk, Dariia; Koelling, Jannes; Lai, Jeremy; Send, Uwe; Wallace, Douglas
2017-04-01
Over the last two decades observing capacity for the global ocean has increased dramatically. Emerging sensor technologies for dissolved gases, nutrients and bio-optical properties in seawater are allowing extension of in situ observations beyond the traditionally measured salinity, temperature and pressure (CTD). However the effort to extend observations using autonomous instruments and platforms carries the risk of losing the level of data quality achievable through conventional water sampling techniques. We will present results from a case study with the SeaCycler profiling winch focusing on quality control of the in-situ measurements. A total of 13 sensors were deployed from May 2016 to early 2017 on SeaCycler's profiling sensor float, including CTD, dissolved oxygen (O2, 3 sensors), carbon dioxide (pCO2, 2 sensors), nutrients, velocity sensors, fluorometer, transmissometer, single channel PAR sensor, and others. We will highlight how multiple measurement technologies (e.g. for O2 and CO2) complement each other and result in a high quality data product. We will also present an initial assessment of the bio-optical data, their implications for seasonal phytoplankton dynamics and comparisons to climatologies and ocean-color data products obtained from the MODIS satellite.
BioCapacitor: A novel principle for biosensors.
Sode, Koji; Yamazaki, Tomohiko; Lee, Inyoung; Hanashi, Takuya; Tsugawa, Wakako
2016-02-15
Studies regarding biofuel cells utilizing biocatalysts such as enzymes and microorganisms as electrocatalysts have been vigorously conducted over the last two decades. Because of their environmental safety and sustainability, biofuel cells are expected to be used as clean power generators. Among several principles of biofuel cells, enzyme fuel cells have attracted significant attention for their use as alternative energy sources for future implantable devices, such as implantable insulin pumps and glucose sensors in artificial pancreas and pacemakers. However, the inherent issue of the biofuel cell principle is the low power of a single biofuel cell. The theoretical voltage of biofuel cells is limited by the redox potential of cofactors and/or mediators employed in the anode and cathode, which are inadequate for operating any devices used for biomedical application. These limitations inspired us to develop a novel biodevice based on an enzyme fuel cell that generates sufficient stable power to operate electric devices, designated "BioCapacitor." To increase voltage, the enzyme fuel cell is connected to a charge pump. To obtain a sufficient power and voltage to operate an electric device, a capacitor is used to store the potential generated by the charge pump. Using the combination of a charge pump and capacitor with an enzyme fuel cell, high voltages with sufficient temporary currents to operate an electric device were generated without changing the design and construction of the enzyme fuel cell. In this review, the BioCapacitor principle is described. The three different representative categories of biodevices employing the BioCapacitor principle are introduced. Further, the recent challenges in the developments of self-powered stand-alone biodevices employing enzyme fuel cells combined with charge pumps and capacitors are introduced. Finally, the future prospects of biodevices employing the BioCapacitor principle are addressed. Copyright © 2015 The Authors
Hybrid organic semiconductor lasers for bio-molecular sensing.
Haughey, Anne-Marie; Foucher, Caroline; Guilhabert, Benoit; Kanibolotsky, Alexander L; Skabara, Peter J; Burley, Glenn; Dawson, Martin D; Laurand, Nicolas
2014-01-01
Bio-functionalised luminescent organic semiconductors are attractive for biophotonics because they can act as efficient laser materials while simultaneously interacting with molecules. In this paper, we present and discuss a laser biosensor platform that utilises a gain layer made of such an organic semiconductor material. The simple structure of the sensor and its operation principle are described. Nanolayer detection is shown experimentally and analysed theoretically in order to assess the potential and the limits of the biosensor. The advantage conferred by the organic semiconductor is explained, and comparisons to laser sensors using alternative dye-doped materials are made. Specific biomolecular sensing is demonstrated, and routes to functionalisation with nucleic acid probes, and future developments opened up by this achievement, are highlighted. Finally, attractive formats for sensing applications are mentioned, as well as colloidal quantum dots, which in the future could be used in conjunction with organic semiconductors.
Printed Multi-Turn Loop Antenna for RF Bio-Telemetry
NASA Technical Reports Server (NTRS)
Simons, Rainee N.; Hall, David G.; Miranda, Felix A.
2004-01-01
In this paper, a novel printed multi-turn loop antenna for contact-less powering and RF telemetry from implantable bio- MEMS sensors at a design frequency of 300 MHz is demonstrated. In addition, computed values of input reactance, radiation resistance, skin effect resistance, and radiation efficiency for the printed multi-turn loop antenna are presented. The computed input reactance is compared with the measured values and shown to be in fair agreement. The computed radiation efficiency at the design frequency is about 24 percent.
Smart single-chip gas sensor microsystem
NASA Astrophysics Data System (ADS)
Hagleitner, C.; Hierlemann, A.; Lange, D.; Kummer, A.; Kerness, N.; Brand, O.; Baltes, H.
2001-11-01
Research activity in chemical gas sensing is currently directed towards the search for highly selective (bio)chemical layer materials, and to the design of arrays consisting of different partially selective sensors that permit subsequent pattern recognition and multi-component analysis. Simultaneous use of various transduction platforms has been demonstrated, and the rapid development of integrated-circuit technology has facilitated the fabrication of planar chemical sensors and sensors based on three-dimensional microelectromechanical systems. Complementary metal-oxide silicon processes have previously been used to develop gas sensors based on metal oxides and acoustic-wave-based sensor devices. Here we combine several of these developments to fabricate a smart single-chip chemical microsensor system that incorporates three different transducers (mass-sensitive, capacitive and calorimetric), all of which rely on sensitive polymeric layers to detect airborne volatile organic compounds. Full integration of the microelectronic and micromechanical components on one chip permits control and monitoring of the sensor functions, and enables on-chip signal amplification and conditioning that notably improves the overall sensor performance. The circuitry also includes analog-to-digital converters, and an on-chip interface to transmit the data to off-chip recording units. We expect that our approach will provide a basis for the further development and optimization of gas microsystems.
Flexible graphene bio-nanosensor for lactate.
Labroo, Pratima; Cui, Yue
2013-03-15
The development of a flexible nanosensor for detecting lactate could expand opportunities for using graphene, both in fundamental studies for a variety of device platforms and in practical applications. Graphene is a delicate single-layer, two-dimensional network of carbon atoms with ultrasensitive sensing capabilities. Lactic acid is important for clinical analysis, sports medicine, and the food industry. Recently, wearable and flexible bioelectronics on plastics have attracted great interest for healthcare, sports and defense applications due to their advantages of being light-weight, bendable, or stretchable. Here, we demonstrate for the first time the development of a flexible graphene-based bio-nanosensor to detect lactate. Our results show that flexible lactate biosensors can be fabricated on a variety of plastic substrates. The sensor can detect lactate sensitively from 0.08 μM to 20 μM with a fast steady-state measuring time of 2s. The sensor can also detect lactate under different mechanical bending conditions, the sensor response decreased as the bending angle and number of bending repetitions increased. We anticipate that these results could open exciting opportunities for fundamental studies of flexible graphene bioelectronics by using other bioreceptors, as well as a variety of wearable, implantable, real-time, or on-site applications in fields ranging from clinical analysis to defense. Copyright © 2012 Elsevier B.V. All rights reserved.
Properties of piezoresistive silicon nano-scale cantilevers with applications to BioNEMS
NASA Astrophysics Data System (ADS)
Arlett, Jessica Lynn
Over the last decade a great deal of interest has been raised in applications of Microelectromechanical Sensors [MEMS] for the detection of biological molecules and to the study of their forces of interaction. Experiments in these areas have included Force Spectroscopy (Chemical Force Microscopy), MEMS patch clamp technology, and surface stress sensors. All of these technologies suffer from limitations on temporal response and involve devices with active surface areas that are large compared to molecular dimensions. Biofunctionalized nanoelectromechanical systems (BioNEMS) have the potential to overcome both of these hurdles, offering important new prospects for single-molecule force assays that are amenable to large scale integration. Results are presented here on the characterization of piezoresistive silicon cantilevers with applications to BioNEMS devices. The cantilevers were characterized by studying their response in gaseous ambients under a number of drive conditions including magnetic, piezoelectric, and thermal actuation, in addition to passive detection of the thermomechanical response. The measurements were performed at liquid helium temperature, at room temperature, and over a range of pressures (atmospheric pressure to 30mT). Theoretical studies have been performed on the response of these devices to Brownian fluctuations in fluid, on the feasibility of these devices as surface stress sensors, and on improvements in device design as compared to piezoresistive surface stress sensors currently discussed in the literature. The devices were encapsulated in microfluidics and measurements were performed to show the noise floor in fluid. The piezoresistive response of the device in fluid was shown through the use of pulsatory fluidic drive. As a proof of concept, biodetection experiments are presented for biotin labeled beads. The biofunctionalization for the latter experiment was performed entirely within the microfluidics. A discussion of how these
Models and signal processing for an implanted ethanol bio-sensor.
Han, Jae-Joon; Doerschuk, Peter C; Gelfand, Saul B; O'Connor, Sean J
2008-02-01
The understanding of drinking patterns leading to alcoholism has been hindered by an inability to unobtrusively measure ethanol consumption over periods of weeks to months in the community environment. An implantable ethanol sensor is under development using microelectromechanical systems technology. For safety and user acceptability issues, the sensor will be implanted subcutaneously and, therefore, measure peripheral-tissue ethanol concentration. Determining ethanol consumption and kinetics in other compartments from the time course of peripheral-tissue ethanol concentration requires sophisticated signal processing based on detailed descriptions of the relevant physiology. A statistical signal processing system based on detailed models of the physiology and using extended Kalman filtering and dynamic programming tools is described which can estimate the time series of ethanol concentration in blood, liver, and peripheral tissue and the time series of ethanol consumption based on peripheral-tissue ethanol concentration measurements.
(Bio)Sensing Using Nanoparticle Arrays: On the Effect of Analyte Transport on Sensitivity.
Lynn, N Scott; Homola, Jiří
2016-12-20
There has recently been an extensive amount of work regarding the development of optical, electrical, and mechanical (bio)sensors employing planar arrays of surface-bound nanoparticles. The sensor output for these systems is dependent on the rate at which analyte is transported to, and interacts with, each nanoparticle in the array. There has so far been little discussion on the relationship between the design parameters of an array and the interplay of convection, diffusion, and reaction. Moreover, current methods providing such information require extensive computational simulation. Here we demonstrate that the rate of analyte transport to a nanoparticle array can be quantified analytically. We show that such rates are bound by both the rate to a single NP and that to a planar surface (having equivalent size as the array), with the specific rate determined by the fill fraction: the ratio between the total surface area used for biomolecular capture with respect to the entire sensing area. We characterize analyte transport to arrays with respect to changes in numerous parameters relevant to experiment, including variation of the nanoparticle shape and size, packing density, flow conditions, and analyte diffusivity. We also explore how analyte capture is dependent on the kinetic parameters related to an affinity-based biosensor, and furthermore, we classify the conditions under which the array might be diffusion- or reaction-limited. The results obtained herein are applicable toward the design and optimization of all (bio)sensors based on nanoparticle arrays.
Biotactile Sensors: Self-Powered Electronic Skin with Biotactile Selectivity (Adv. Mater. 18/2016).
Hu, Kesong; Xiong, Rui; Guo, Hengyu; Ma, Ruilong; Zhang, Shuaidi; Wang, Zhong Lin; Tsukruk, Vladimir V
2016-05-01
On page 3549, V. V. Tsukruk and co-workers develop self-powered ultrathin flexible films for bio-tactile detection. Graphene oxide materials are engineered for robust self-powered tactile sensing applications harnessing their electrochemical reactivity. The simple quadruple electronic skin sensor can recognize nine spatial bio-tactile positions with high sensitivity and selectivity-an approach that can be expanded towards large-area flexible skin arrays. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Areizaga-Martinez, Hector I.; Kravchenko, Ivan; Lavrik, Nickolay V.; ...
2016-08-26
The fabrication of high-performance plasmonic nanomaterials for bio-sensing and trace chemical detection is a field of intense theoretical and experimental research. The use of metal-silicon nanopillar arrays as analytical sensors has been reported with reasonable results in recent years. The use of bio-inspired nanocomposite structures that follow the Fibonacci numerical architecture offers the opportunity to develop nanostructures with theoretically higher and more reproducible plasmonic fields over extended areas. The work presented here describes the nanofabrication process for a series of 40 µm × 40 µm bio-inspired arrays classified as asymmetric fractals (sunflower seeds and romanesco broccoli), bilaterally symmetric (acacia leavesmore » and honeycombs), and radially symmetric (such as orchids and lily flowers) using electron beam lithography. In addition, analytical capabilities were evaluated using surface-enhanced Raman scattering (SERS). Here, the substrate characterization and SERS performance of the developed substrates as the strategies to assess the design performance are presented and discussed.« less
DOE Office of Scientific and Technical Information (OSTI.GOV)
Areizaga-Martinez, Hector I.; Kravchenko, Ivan; Lavrik, Nickolay V.
The fabrication of high-performance plasmonic nanomaterials for bio-sensing and trace chemical detection is a field of intense theoretical and experimental research. The use of metal-silicon nanopillar arrays as analytical sensors has been reported with reasonable results in recent years. The use of bio-inspired nanocomposite structures that follow the Fibonacci numerical architecture offers the opportunity to develop nanostructures with theoretically higher and more reproducible plasmonic fields over extended areas. The work presented here describes the nanofabrication process for a series of 40 µm × 40 µm bio-inspired arrays classified as asymmetric fractals (sunflower seeds and romanesco broccoli), bilaterally symmetric (acacia leavesmore » and honeycombs), and radially symmetric (such as orchids and lily flowers) using electron beam lithography. In addition, analytical capabilities were evaluated using surface-enhanced Raman scattering (SERS). Here, the substrate characterization and SERS performance of the developed substrates as the strategies to assess the design performance are presented and discussed.« less
Areizaga-Martinez, Hector I; Kravchenko, Ivan; Lavrik, Nickolay V; Sepaniak, Michael J; Hernández-Rivera, Samuel P; De Jesús, Marco A
2016-09-01
The fabrication of high-performance plasmonic nanomaterials for bio-sensing and trace chemical detection is a field of intense theoretical and experimental research. The use of metal-silicon nanopillar arrays as analytical sensors has been reported with reasonable results in recent years. The use of bio-inspired nanocomposite structures that follow the Fibonacci numerical architecture offers the opportunity to develop nanostructures with theoretically higher and more reproducible plasmonic fields over extended areas. The work presented here describes the nanofabrication process for a series of 40 µm × 40 µm bio-inspired arrays classified as asymmetric fractals (sunflower seeds and romanesco broccoli), bilaterally symmetric (acacia leaves and honeycombs), and radially symmetric (such as orchids and lily flowers) using electron beam lithography. In addition, analytical capabilities were evaluated using surface-enhanced Raman scattering (SERS). The substrate characterization and SERS performance of the developed substrates as the strategies to assess the design performance are presented and discussed. © The Author(s) 2016.
An epidemic model for biological data fusion in ad hoc sensor networks
NASA Astrophysics Data System (ADS)
Chang, K. C.; Kotari, Vikas
2009-05-01
Bio terrorism can be a very refined and a catastrophic approach of attacking a nation. This requires the development of a complete architecture dedicatedly designed for this purpose which includes but is not limited to Sensing/Detection, Tracking and Fusion, Communication, and others. In this paper we focus on one such architecture and evaluate its performance. Various sensors for this specific purpose have been studied. The accent has been on use of Distributed systems such as ad-hoc networks and on application of epidemic data fusion algorithms to better manage the bio threat data. The emphasis has been on understanding the performance characteristics of these algorithms under diversified real time scenarios which are implemented through extensive JAVA based simulations. Through comparative studies on communication and fusion the performance of channel filter algorithm for the purpose of biological sensor data fusion are validated.
Measuring (bio)physical tree properties using accelerometers
NASA Astrophysics Data System (ADS)
van Emmerik, Tim; Steele-Dunne, Susan; Hut, Rolf; Gentine, Pierre; Selker, John; van de Giesen, Nick
2017-04-01
Trees play a crucial role in the water, carbon and nitrogen cycle on local, regional and global scales. Understanding the exchange of heat, water, and CO2 between trees and the atmosphere is important to assess the impact of drought, deforestation and climate change. Unfortunately, ground measurements of tree dynamics are often expensive, or difficult due to challenging environments. We demonstrate the potential of measuring (bio)physical properties of trees using robust and affordable acceleration sensors. Tree sway is dependent on e.g. mass and wind energy absorption of the tree. By measuring tree acceleration we can relate the tree motion to external loads (e.g. precipitation), and tree (bio)physical properties (e.g. mass). Using five months of acceleration data of 19 trees in the Brazilian Amazon, we show that the frequency spectrum of tree sway is related to mass, precipitation, and canopy drag. This presentation aims to show the concept of using accelerometers to measure tree dynamics, and we acknowledge that the presented example applications is not an exhaustive list. Further analyses are the scope of current research, and we hope to inspire others to explore additional applications.
Gil, Yeongjoon; Wu, Wanqing; Lee, Jungtae
2012-01-01
Background Human life can be further improved if diseases and disorders can be predicted before they become dangerous, by correctly recognizing signals from the human body, so in order to make disease detection more precise, various body-signals need to be measured simultaneously in a synchronized manner. Object This research aims at developing an integrated system for measuring four signals (EEG, ECG, respiration, and PPG) and simultaneously producing synchronous signals on a Wireless Body Sensor Network. Design We designed and implemented a platform for multiple bio-signals using Bluetooth communication. Results First, we developed a prototype board and verified the signals from the sensor platform using frequency responses and quantities. Next, we designed and implemented a lightweight, ultra-compact, low cost, low power-consumption Printed Circuit Board. Conclusion A synchronous multi-body sensor platform is expected to be very useful in telemedicine and emergency rescue scenarios. Furthermore, this system is expected to be able to analyze the mutual effects among body signals. PMID:23112605
Bermuda Bio Optics Project. Chapter 14
NASA Technical Reports Server (NTRS)
Nelson, Norm
2003-01-01
The Bermuda BioOptics Project (BBOP) is a collaborative effort between the Institute for Computational Earth System Science (ICESS) at the University of California at Santa Barbara (UCSB) and the Bermuda Biological Station for Research (BBSR). This research program is designed to characterize light availability and utilization in the Sargasso Sea, and to provide an optical link by which biogeochemical observations may be used to evaluate bio-optical models for pigment concentration, primary production, and sinking particle fluxes from satellite-based ocean color sensors. The BBOP time-series was initiated in 1992, and is carried out in conjunction with the U.S. JGOFS Bermuda Atlantic Time-series Study (BATS) at the Bermuda Biological Station for Research. The BATS program itself has been observing biogeochemical processes (primary productivity, particle flux and elemental cycles) in the mesotrophic waters of the Sargasso Sea since 1988. Closely affiliated with BBOP and BATS is a separate NASA-funded study of the spatial variability of biogeochemical processes in the Sargasso Sea using high-resolution AVHRR and SeaWiFS data collected at Bermuda (N. Nelson, P.I.). The collaboration between BATS and BBOP measurements has resulted in a unique data set that addresses not only the SIMBIOS goals but also the broader issues of important factors controlling the carbon cycle.
Chang, Chia-Lin; Chang, Chih-Wei; Huang, Hong-Yi; Hsu, Chen-Ming; Huang, Chia-Hsuan; Chiou, Jin-Chern; Luo, Ching-Hsing
2010-01-01
This work describes a power-efficient bio-potential acquisition device for long-term healthcare applications that is implemented using novel microelectromechanical dry electrodes (MDE) and a low power bio-potential processing chip. Using micromachining technology, an attempt is also made to enhance the sensing reliability and stability by fabricating a diamond-shaped MDE (DS-MDE) that has a satisfactory self-stability capability and superior electric conductivity when attached onto skin without any extra skin tissue injury technology. To acquire differential bio-potentials such as ECG signals, the proposed processing chip fabricated in a standard CMOS process has a high common mode rejection ratio (C.M.R.R.) differential amplifier and a 12-bit analog-to-digital converter (ADC). Use of the proposed system and integrate simple peripheral commercial devices can obtain the ECG signal efficiently without additional skin tissue injury and ensure continuous monitoring more than 70 hours with a 400 mAh battery. PMID:22399907
Chang, Chia-Lin; Chang, Chih-Wei; Huang, Hong-Yi; Hsu, Chen-Ming; Huang, Chia-Hsuan; Chiou, Jin-Chern; Luo, Ching-Hsing
2010-01-01
This work describes a power-efficient bio-potential acquisition device for long-term healthcare applications that is implemented using novel microelectromechanical dry electrodes (MDE) and a low power bio-potential processing chip. Using micromachining technology, an attempt is also made to enhance the sensing reliability and stability by fabricating a diamond-shaped MDE (DS-MDE) that has a satisfactory self-stability capability and superior electric conductivity when attached onto skin without any extra skin tissue injury technology. To acquire differential bio-potentials such as ECG signals, the proposed processing chip fabricated in a standard CMOS process has a high common mode rejection ratio (C.M.R.R.) differential amplifier and a 12-bit analog-to-digital converter (ADC). Use of the proposed system and integrate simple peripheral commercial devices can obtain the ECG signal efficiently without additional skin tissue injury and ensure continuous monitoring more than 70 hours with a 400 mAh battery.
NASA Astrophysics Data System (ADS)
Strong, Anita A.; Stimpson, Donald I.; Bartholomew, Dwight U.; Jenkins, Thomas F.; Elkind, Jerome L.
1999-08-01
An antibody-based competition assay has been developed using a surface plasmon resonance (SPR) sensor platform for the detection of trinitrotoluene (TNT) in soil extract solutions. The objective of this work is to develop a sensor-based assay technology to use in the field for real- time detection of land mines. This immunoassay combines very simple bio-film attachment procedures and a low-cost SPR sensor design to detect TNT in soil extracts. The active bio-surface is a coating of bovine serum albumin that has been decorated with trinitrobenzene groups. A blind study on extracts from a large soil matrix was recently performed and result from this study will be presented. Potential interferant studied included 2,4-dinitrophenol, 2,4- dinitrotoluene, ammonium nitrate, and 2,4- dichlorophenoxyacetic acid. Cross-reactivity with dinitrotoluene will be discussed. Also, plans to reach sensitivity levels of 1ppb TNT in soil will be described.
Review of Recent Metamaterial Microfluidic Sensors.
Salim, Ahmed; Lim, Sungjoon
2018-01-15
Metamaterial elements/arrays exhibit a sensitive response to fluids yet with a small footprint, therefore, they have been an attractive choice to realize various sensing devices when integrated with microfluidic technology. Micro-channels made from inexpensive biocompatible materials avoid any contamination from environment and require only microliter-nanoliter sample for sensing. Simple design, easy fabrication process, light weight prototype, and instant measurements are advantages as compared to conventional (optical, electrochemical and biological) sensing systems. Inkjet-printed flexible sensors find their utilization in rapidly growing wearable electronics and health-monitoring flexible devices. Adequate sensitivity and repeatability of these low profile microfluidic sensors make them a potential candidate for point-of-care testing which novice patients can use reliably. Aside from degraded sensitivity and lack of selectivity in all practical microwave chemical sensors, they require an instrument, such as vector network analyzer for measurements and not readily available as a self-sustained portable sensor. This review article presents state-of-the-art metamaterial inspired microfluidic bio/chemical sensors (passive devices ranging from gigahertz to terahertz range) with an emphasis on metamaterial sensing circuit and microfluidic detection. We also highlight challenges and strategies to cope these issues which set future directions.
Bio-inspired piezoelectric artificial hair cell sensor fabricated by powder injection molding
NASA Astrophysics Data System (ADS)
Han, Jun Sae; Oh, Keun Ha; Moon, Won Kyu; Kim, Kyungseop; Joh, Cheeyoung; Seo, Hee Seon; Bollina, Ravi; Park, Seong Jin
2015-12-01
A piezoelectric artificial hair cell sensor was fabricated by the powder injection molding process in order to make an acoustic vector hydrophone. The entire process of powder injection molding was developed and optimized for PMN-PZT ceramic powder. The artificial hair cell sensor, which consists of high aspect ratio hair cell and three rectangular mechanoreceptors, was precisely fabricated through the developed powder injection molding process. The density and the dielectric property of the fabricated sensor shows 98% of the theoretical density and 85% of reference dielectric property of PMN-PZT ceramic powder. With regard to homogeneity, three rectangular mechanoreceptors have the same dimensions, with 3 μm of tolerance with 8% of deviation of dielectric property. Packaged vector hydrophones measure the underwater acoustic signals from 500 to 800 Hz with -212 dB of sensitivity. Directivity of vector hydrophone was acquired at 600 Hz as analyzing phase differences of electric signals.
D-Amino acid oxidase bio-functionalized platforms: Toward an enhanced enzymatic bio-activity
NASA Astrophysics Data System (ADS)
Herrera, Elisa; Valdez Taubas, Javier; Giacomelli, Carla E.
2015-11-01
The purpose of this work is to study the adsorption process and surface bio-activity of His-tagged D-amino acid oxidase (DAAO) from Rhodotorula gracilis (His6-RgDAAO) as the first step for the development of an electrochemical bio-functionalized platform. With such a purpose this work comprises: (a) the His6-RgDAAO bio-activity in solution determined by amperometry, (b) the adsorption mechanism of His6-RgDAAO on bare gold and carboxylated modified substrates in the absence (substrate/COO-) and presence of Ni(II) (substrate/COO- + Ni(II)) determined by reflectometry, and (c) the bio-activity of the His6-RgDAAO bio-functionalized platforms determined by amperometry. Comparing the adsorption behavior and bio-activity of His6-RgDAAO on these different solid substrates allows understanding the contribution of the diverse interactions responsible for the platform performance. His6-RgDAAO enzymatic performance in solution is highly improved when compared to the previously used pig kidney (pk) DAAO. His6-RgDAAO exhibits an amperometrically detectable bio-activity at concentrations as low as those expected on a bio-functional platform; hence, it is a viable bio-recognition element of D-amino acids to be coupled to electrochemical platforms. Moreover, His6-RgDAAO bio-functionalized platforms exhibit a higher surface activity than pkDAAO physically adsorbed on gold. The platform built on Ni(II) modified substrates present enhanced bio-activity because the surface complexes histidine-Ni(II) provide with site-oriented, native-like enzymes. The adsorption mechanism responsible of the excellent performance of the bio-functionalized platform takes place in two steps involving electrostatic and bio-affinity interactions whose prevalence depends on the degree of surface coverage.
1 kHz 2D Visual Motion Sensor Using 20 × 20 Silicon Retina Optical Sensor and DSP Microcontroller.
Liu, Shih-Chii; Yang, MinHao; Steiner, Andreas; Moeckel, Rico; Delbruck, Tobi
2015-04-01
Optical flow sensors have been a long running theme in neuromorphic vision sensors which include circuits that implement the local background intensity adaptation mechanism seen in biological retinas. This paper reports a bio-inspired optical motion sensor aimed towards miniature robotic and aerial platforms. It combines a 20 × 20 continuous-time CMOS silicon retina vision sensor with a DSP microcontroller. The retina sensor has pixels that have local gain control and adapt to background lighting. The system allows the user to validate various motion algorithms without building dedicated custom solutions. Measurements are presented to show that the system can compute global 2D translational motion from complex natural scenes using one particular algorithm: the image interpolation algorithm (I2A). With this algorithm, the system can compute global translational motion vectors at a sample rate of 1 kHz, for speeds up to ±1000 pixels/s, using less than 5 k instruction cycles (12 instructions per pixel) per frame. At 1 kHz sample rate the DSP is 12% occupied with motion computation. The sensor is implemented as a 6 g PCB consuming 170 mW of power.
tmBioC: improving interoperability of text-mining tools with BioC.
Khare, Ritu; Wei, Chih-Hsuan; Mao, Yuqing; Leaman, Robert; Lu, Zhiyong
2014-01-01
The lack of interoperability among biomedical text-mining tools is a major bottleneck in creating more complex applications. Despite the availability of numerous methods and techniques for various text-mining tasks, combining different tools requires substantial efforts and time owing to heterogeneity and variety in data formats. In response, BioC is a recent proposal that offers a minimalistic approach to tool interoperability by stipulating minimal changes to existing tools and applications. BioC is a family of XML formats that define how to present text documents and annotations, and also provides easy-to-use functions to read/write documents in the BioC format. In this study, we introduce our text-mining toolkit, which is designed to perform several challenging and significant tasks in the biomedical domain, and repackage the toolkit into BioC to enhance its interoperability. Our toolkit consists of six state-of-the-art tools for named-entity recognition, normalization and annotation (PubTator) of genes (GenNorm), diseases (DNorm), mutations (tmVar), species (SR4GN) and chemicals (tmChem). Although developed within the same group, each tool is designed to process input articles and output annotations in a different format. We modify these tools and enable them to read/write data in the proposed BioC format. We find that, using the BioC family of formats and functions, only minimal changes were required to build the newer versions of the tools. The resulting BioC wrapped toolkit, which we have named tmBioC, consists of our tools in BioC, an annotated full-text corpus in BioC, and a format detection and conversion tool. Furthermore, through participation in the 2013 BioCreative IV Interoperability Track, we empirically demonstrate that the tools in tmBioC can be more efficiently integrated with each other as well as with external tools: Our experimental results show that using BioC reduces >60% in lines of code for text-mining tool integration. The tmBioC toolkit
Bio-oil fractionation and condensation
Brown, Robert C; Jones, Samuel T; Pollard, Anthony
2013-07-02
A method of fractionating bio-oil vapors which involves providing bio-oil vapors comprising bio-oil constituents is described. The bio-oil vapors are cooled in a first stage which comprises a condenser having passages for the bio-oil separated by a heat conducting wall from passages for a coolant. The coolant in the condenser of the first stage is maintained at a substantially constant temperature, set at a temperature in the range of 75 to 100.degree. C., to condense a first liquid fraction of liquefied bio-oil constituents in the condenser of the first stage. The first liquid fraction of liquified bio-oil constituents from the condenser in the first stage is collected. Also described are steps for subsequently recovering further liquid fractions of liquefied bio-oil constituents. Particular compositions of bio-oil condensation products are also described.
Long period fiber grating based sensor for the detection of triacylglycerides.
Baliyan, Anjli; Sital, Shivani; Tiwari, Umesh; Gupta, Rani; Sharma, Enakshi K
2016-05-15
In this paper, stable, label free enzyme based sensor using long period fiber grating (LPG) is described for the detection of triacylglycerides. A stable covalent binding technique for lipase enzyme immobilization on an optical fiber is reported. An active and stable attachment of the functional group of the enzyme on the fiber surface is achieved using this method. Enzyme immobilization is confirmed by Scanning Electron Microscopy (SEM) and Raman Spectroscopy. The stability is confirmed by lipase p-nitrophenyl palmitate (PNP) assay. In contrast to widely used amperometric based biosensor, where a number of enzymes are required, only one enzyme, namely, lipase is required in our sensor. The sensor shows optimum response within one minute at a temperature of 37°C and pH of 7.4. The sensor is based on the shift in resonance wavelength of the LPG transmission spectrum due to the interaction of triacylglycerides with the enzyme. The biosensor is highly specific towards triacylglycerides and is unaffected by the presence of many other interfering substances in serum. Interaction between the bio-molecules and the long period grating surface is also modeled theoretically using a four layer model for the LPG fiber with the bio-recognition layer and the results obtained are consistent with experimentally obtained results. The sensor shows a high sensitivity of 0.5 nm/mM and a low detection limit of 17.71 mg/dl for the physiological range of triacylglycerides in human blood. Copyright © 2015 Elsevier B.V. All rights reserved.
Combinatorial Nano-Bio Interfaces.
Cai, Pingqiang; Zhang, Xiaoqian; Wang, Ming; Wu, Yun-Long; Chen, Xiaodong
2018-06-08
Nano-bio interfaces are emerging from the convergence of engineered nanomaterials and biological entities. Despite rapid growth, clinical translation of biomedical nanomaterials is heavily compromised by the lack of comprehensive understanding of biophysicochemical interactions at nano-bio interfaces. In the past decade, a few investigations have adopted a combinatorial approach toward decoding nano-bio interfaces. Combinatorial nano-bio interfaces comprise the design of nanocombinatorial libraries and high-throughput bioevaluation. In this Perspective, we address challenges in combinatorial nano-bio interfaces and call for multiparametric nanocombinatorics (composition, morphology, mechanics, surface chemistry), multiscale bioevaluation (biomolecules, organelles, cells, tissues/organs), and the recruitment of computational modeling and artificial intelligence. Leveraging combinatorial nano-bio interfaces will shed light on precision nanomedicine and its potential applications.
Direct optical sensors: principles and selected applications.
Gauglitz, Guenter
2005-01-01
In the field of bio and chemosensors a large number of detection principles has been published within the last decade. These detection principles are based either on the observation of fluorescence-labelled systems or on direct optical detection in the heterogeneous phase. Direct optical detection can be measured by remission (absorption of reflected radiation, opt(r)odes), by measuring micro-refractivity, or measuring interference. In the last case either Mach-Zehnder interferometers or measurement of changes in the physical thickness of the layer (measuring micro-reflectivity) caused, e.g., by swelling effects in polymers (due to interaction with analytes) or in bioassays (due to affinity reactions) also play an important role. Here, an overview of methods of microrefractometric and microreflectometric principles is given and benefits and drawbacks of the various approaches are demonstrated using samples from the chemo and biosensor field. The quality of sensors does not just depend on transduction principles but on the total sensor system defined by this transduction, the sensitive layer, data acquisition electronics, and evaluation software. The intention of this article is, therefore, to demonstrate the essentials of the interaction of these parts within the system, and the focus is on optical sensing using planar transducers, because fibre optical sensors have been reviewed in this journal only recently. Lack of selectivity of chemosensors can be compensated either by the use of sensor arrays or by evaluating time-resolved measurements of analyte/sensitive layer interaction. In both cases chemometrics enables the quantification of analyte mixtures. These data-processing methods have also been successfully applied to antibody/antigen interactions even using cross-reactive antibodies. Because miniaturisation and parallelisation are essential approaches in recent years, some aspects and current trends, especially for bio-applications, will be discussed
Development of a Bioaerosol single particle detector (BIO IN) for the Fast Ice Nucleus CHamber FINCH
NASA Astrophysics Data System (ADS)
Bundke, U.; Reimann, B.; Nillius, B.; Jaenicke, R.; Bingemer, H.
2010-02-01
In this work we present the setup and first tests of our new BIO IN detector. This detector was constructed to classify atmospheric ice nuclei (IN) for their biological content. It is designed to be coupled to the Fast Ice Nucleus CHamber FINCH. If one particle acts as an ice nucleus, it will be at least partly covered with ice at the end of the development section of the FINCH chamber. The device combines an auto-fluorescence detector and a circular depolarization detector for simultaneous detection of biological material and discrimination between water droplets, ice crystals and non activated large aerosol particles. The excitation of biological material with UV light and analysis of auto-fluorescence is a common principle used for flow cytometry, fluorescence microscopy, spectroscopy and imaging. The detection of auto-fluorescence of airborne single particles demands some more experimental effort. However, expensive commercial sensors are available for special purposes, e.g. size distribution measurements. But these sensors will not fit the specifications needed for the FINCH IN counter (e.g. high sample flow of up 10 LPM). The newly developed -low cost- BIO IN sensor uses a single high-power UV LED for the electronic excitation instead of much more expensive UV lasers. Other key advantages of the new sensor are the low weight, compact size, and the little effect on the aerosol sample, which allows it to be coupled with other instruments for further analysis. The instrument will be flown on one of the first missions of the new German research aircraft "HALO" (High Altitude and LOng range).
Bio-oil fractionation and condensation
DOE Office of Scientific and Technical Information (OSTI.GOV)
Brown, Robert C.; Jones, Samuel T.; Pollard, Anthony
The present invention relates to a method of fractionating bio-oil vapors which involves providing bio-oil vapors comprising bio-oil constituents. The bio-oil vapors are cooled in a first stage which comprises a condenser having passages for the bio-oil separated by a heat conducting wall from passages for a coolant. The coolant in the condenser of the first stage is maintained at a substantially constant temperature, set at a temperature in the range of 75 to 100.degree. C., to condense a first liquid fraction of liquefied bio-oil constituents in the condenser of the first stage. The first liquid fraction of liquified bio-oilmore » constituents from the condenser in the first stage is collected. Also disclosed are steps for subsequently recovering further liquid fractions of liquefied bio-oil constituents. Particular compositions of bio-oil condensation products are also described.« less
Implantable Myoelectric Sensors (IMESs) for Intramuscular Electromyogram Recording
Weir, Richard F. ff.; Troyk, Phil R.; DeMichele, Glen A.; Kerns, Douglas A.; Schorsch, Jack F.; Maas, Huub
2011-01-01
We have developed a multichannel electrogmyography sensor system capable of receiving and processing signals from up to 32 implanted myoelectric sensors (IMES). The appeal of implanted sensors for myoelectric control is that electromyography (EMG) signals can be measured at their source providing relatively cross-talk-free signals that can be treated as independent control sites. An external telemetry controller receives telemetry sent over a transcutaneous magnetic link by the implanted electrodes. The same link provides power and commands to the implanted electrodes. Wireless telemetry of EMG signals from sensors implanted in the residual musculature eliminates the problems associated with percutaneous wires, such as infection, breakage, and marsupialization. Each implantable sensor consists of a custom-designed application-specified integrated circuit that is packaged into a bio-compatible RF BION capsule from the Alfred E. Mann Foundation. Implants are designed for permanent long-term implantation with no servicing requirements. We have a fully operational system. The system has been tested in animals. Implants have been chronically implanted in the legs of three cats and are still completely operational four months after implantation. PMID:19224729
Islam, Afroja T; Siddique, Ariful H; Ramulu, T S; Reddy, Venu; Eu, Young-Jae; Cho, Seung Hyun; Kim, CheolGi
2012-12-01
In this work, we demonstrated the alignment of polystyrene latex microspheres (diameter of 1 ~45 μm), bio-functionalized superparamagnetic beads (diameter 2.8 μm), and live cells (average diameter 1 ~2 μm) using an ultrasonic standing wave (USW) in a PDMS microfluidic channel (330 μm width) attached on a Si substrate for bio-medical applications. To generate a standing wave inside the channel, ultrasound of 2.25 MHz resonance frequency (for the channel width) was applied by two ultrasound transducers installed at both sides of the channel which caused the radiation force to concentrate the micro-particles at the single pressure nodal plane of USW. By increasing the frequency to the next resonance condition of the channel, the particles were concentrated in dual nodal planes. Migration time of the micro-particles towards the single nodal plane was recorded as 108 s, 17 s, and 115 s for polystyrene particles of 2 μm diameter, bio-functionalized magnetic beads, and live cells, respectively. These successful alignments of the bio-functionalized magnetic beads along the desired part of the channel can enhance the performance of a sensor which is applicable for the bio-hybrid system and the alignment of live cells without any damage can be used for sample pre-treatment for the application of lab-on-a-chip type bioassays.
Yin, Sudong; Dolan, Ryan; Harris, Matt; Tan, Zhongchao
2010-05-01
In this study, cattle manure was converted to bio-oil by subcritical hydrothermal liquefaction in the presence of NaOH. The effects of conversion temperature, process gas, initial conversion pressure, residence time and mass ratio of cattle manure to water on the bio-oil yield were studied. The bio-oil was characterized in terms of elemental composition, higher heating value, ultraviolet-visible (UV/Vis) spectroscopy, Fourier transform infrared spectroscopy (FTIR), gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). Results showed that the bio-oil yield depended on the conversion temperature and the process gas. Higher initial conversion pressure, longer residence time and larger mass ratio of cattle manure to water, however, had negative impacts on the bio-oil yield. The higher heating value of bio-oil was 35.53MJ/kg on average. The major non-polar components of bio-oil were toluene, ethyl benzene and xylene, which are components of crude oil, gasoline and diesel. Copyright 2010 Elsevier Ltd. All rights reserved.
Sensor Systems for Space Life Sciences
NASA Technical Reports Server (NTRS)
Somps, Chris J.; Hines, John W.; Connolly, John P. (Technical Monitor)
1995-01-01
Sensors 2000! (S2K!) is a NASA Ames Research Center engineering initiative designed to provide biosensor and bio-instrumentation systems technology expertise to NASA's life sciences spaceflight programs. S2K! covers the full spectrum of sensor technology applications, ranging from spaceflight hardware design and fabrication to advanced technology development, transfer and commercialization. S2K! is currently developing sensor systems for space biomedical applications on BION (a Russian biosatellite focused on Rhesus Monkey physiology) and NEUROLAB (a Space Shuttle flight devoted to neuroscience). It's Advanced Technology Development-Biosensors (ATD-B) project focuses efforts in five principle areas: biotelemetry Systems, chemical and biological sensors, physiological sensors, advanced instrumentation architectures, and data and information management. Technologies already developed and tested included, application-specific sensors, preamplifier hybrids, modular programmable signal conditioners, power conditioning and distribution systems, and a fully implantable dual channel biotelemeter. Systems currently under development include a portable receiver system compatible with an off-the-shelf analog biotelemeter, a 4 channel digital biotelemetry system which monitors pH, a multichannel, g-processor based PCM biotelemetry system, and hand-held personal monitoring systems. S2K! technology easily lends itself to telescience and telemedicine applications as a front-end measurement and data acquisition device, suitable for obtaining and configuring physiological information, and processing that information under control from a remote location.
A smart indoor air quality sensor network
NASA Astrophysics Data System (ADS)
Wen, Jin
2006-03-01
The indoor air quality (IAQ) has an important impact on public health. Currently, the indoor air pollution, caused by gas, particle, and bio-aerosol pollutants, is considered as the top five environmental risks to public health and has an estimated cost of $2 billion/year due to medical cost and lost productivity. Furthermore, current buildings are especially vulnerable for chemical and biological warfare (CBW) agent contamination because the central air conditioning and ventilation system serve as a nature carrier to spread the released agent from one location to the whole indoor environment within a short time period. To assure the IAQ and safety for either new or existing buildings, real time comprehensive IAQ and CBW measurements are needed. With the development of new sensing technologies, economic and reliable comprehensive IAQ and CBW sensors become promising. However, few studies exist that examine the design and evaluation issues related to IAQ and CBW sensor network. In this paper, relevant research areas including IAQ and CBW sensor development, demand control ventilation, indoor CBW sensor system design, and sensor system design for other areas such as water system protection, fault detection and diagnosis, are reviewed and summarized. Potential research opportunities for IAQ and CBW sensor system design and evaluation are discussed.
Photobiology of Symbiodinium revisited: bio-physical and bio-optical signatures
NASA Astrophysics Data System (ADS)
Hennige, S. J.; Suggett, D. J.; Warner, M. E.; McDougall, K. E.; Smith, D. J.
2009-03-01
Light is often the most abundant resource within the nutrient-poor waters surrounding coral reefs. Consequently, zooxanthellae ( Symbiodinium spp.) must continually photoacclimate to optimise productivity and ensure coral success. In situ coral photobiology is becoming dominated by routine assessments using state-of-the-art non-invasive bio-optical or chlorophyll a fluorescence (bio-physical) techniques. Multiple genetic types of Symbiodinium are now known to exist; however, little focus has been given as to how these types differ in terms of characteristics that are observable using these techniques. Therefore, this investigation aimed to revisit and expand upon a pivotal study by Iglesias-Prieto and Trench (1994) by comparing the photoacclimation characteristics of different Symbiodinium types based on their bio-physical (chlorophyll a fluorescence, reaction centre counts) and bio-optical (optical absorption, pigment concentrations) ‘signatures’. Signatures described here are unique to Symbiodinium type and describe phenotypic responses to set conditions, and hence are not suitable to describe taxonomic structure of in hospite Symbiodinium communities. In this study, eight Symbiodinium types from clades and sub-clades (A-B, F) were grown under two PFDs (Photon Flux Density) and examined. The photoacclimation response by Symbiodinium was highly variable between algal types for all bio-physical and for many bio-optical measurements; however, a general preference to modifying reaction centre content over effective antennae-absorption was observed. Certain bio-optically derived patterns, such as light absorption, were independent of algal type and, when considered per photosystem, were matched by reaction centre stoichiometry. Only by better understanding genotypic and phenotypic variability between Symbiodinium types can future studies account for the relative taxonomic and physiological contribution by Symbiodinium to coral acclimation.
Review of Recent Metamaterial Microfluidic Sensors
Salim, Ahmed
2018-01-01
Metamaterial elements/arrays exhibit a sensitive response to fluids yet with a small footprint, therefore, they have been an attractive choice to realize various sensing devices when integrated with microfluidic technology. Micro-channels made from inexpensive biocompatible materials avoid any contamination from environment and require only microliter–nanoliter sample for sensing. Simple design, easy fabrication process, light weight prototype, and instant measurements are advantages as compared to conventional (optical, electrochemical and biological) sensing systems. Inkjet-printed flexible sensors find their utilization in rapidly growing wearable electronics and health-monitoring flexible devices. Adequate sensitivity and repeatability of these low profile microfluidic sensors make them a potential candidate for point-of-care testing which novice patients can use reliably. Aside from degraded sensitivity and lack of selectivity in all practical microwave chemical sensors, they require an instrument, such as vector network analyzer for measurements and not readily available as a self-sustained portable sensor. This review article presents state-of-the-art metamaterial inspired microfluidic bio/chemical sensors (passive devices ranging from gigahertz to terahertz range) with an emphasis on metamaterial sensing circuit and microfluidic detection. We also highlight challenges and strategies to cope these issues which set future directions. PMID:29342953
NASA Astrophysics Data System (ADS)
Pramanik, A.; Biswas, S.; Kumbhakar, P.
2018-02-01
Recently studies on synthesis and fluorescence based sensing in biocompatible carbon quantum dots (CQDs) have become a widely spoken topic of research due to the several advantageous properties of CQDs in compared to semiconductor quantum dots. In this work, we have reported the rarely reported solvatochromism along-with a high photoluminescence (PL) quantum yield (PLQY) of 22%. Samples have been synthesized by using a simple process of hydrothermal carbonization of a naturally occurring bio-waste i.e. Aegle marmelos leaves powder. The linear absorption and PL emission characteristics of CQDs have been studied in different solvent environments to explore the origin of the observed excitation dependent PL emissions characteristics of the sample. The interesting solvatochromic PL (SPL) behavior of CQDs are observed at an excitation wavelength of 325 nm by dispersing them in different polar protic and aprotic solvents, which suggest their possible applications as a replacement of solvatochromic dye molecules for sensing applications. Different polarity functions and molecular-microscopic solvent polarity parameter (ETN) are used to calculate the change in dipole moment (Δδ) of the solute-solvent system and the origin of SPL in CQDs has been explained. The SPL behavior of CQDs has been utilized for fluorescence sensing of organic liquids (Ethanol and Tetrahydrofuran) in water. Whereas, the photo-induced electron transfer mediated quenching in PL of aqueous dispersion of CQDs has led to development of ;turn off; fluorescence Fe3 + ion sensor with a detection limit of 0.12 μM. Therefore, this work may open a new avenue of conversion of a bio-waste into a fluorescent bio-asset.
NASA Technical Reports Server (NTRS)
Mueller, J. L. (Editor); Fargion, Giulietta S. (Editor); McClain, Charles R. (Editor)
2003-01-01
This document stipulates protocols for measuring bio-optical and radiometric data for the Sensor Intercomparison and Merger for Biological and Interdisciplinary Oceanic Studies (SIMBIOS) Project activities and algorithm development. The document is organized into 6 separate volumes as Ocean Optics Protocols for Satellite Ocean Color Sensor Validation, Revision 4. Volume I: Introduction, Background and Conventions; Volume II: Instrument Specifications, Characterization and Calibration; Volume III: Radiometric Measurements and Data Analysis Methods; Volume IV: Inherent Optical Properties: Instruments, Characterization, Field Measurements and Data Analysis Protocols; Volume V: Biogeochemical and Bio-Optical Measurements and Data Analysis Methods; Volume VI: Special Topics in Ocean Optics Protocols and Appendices. The earlier version of Ocean Optics Protocols for Satellite Ocean Color Sensor Validation, Revision 3 (Mueller and Fargion 2002, Volumes 1 and 2) is entirely superseded by the six volumes of Revision 4 listed above.
Real-time biochemical sensor based on Raman scattering with CMOS contact imaging.
Muyun Cao; Yuhua Li; Yadid-Pecht, Orly
2015-08-01
This work presents a biochemical sensor based on Raman scattering with Complementary metal-oxide-semiconductor (CMOS) contact imaging. This biochemical optical sensor is designed for detecting the concentration of solutions. The system is built with a laser diode, an optical filter, a sample holder and a commercial CMOS sensor. The output of the system is analyzed by an image processing program. The system provides instant measurements with a resolution of 0.2 to 0.4 Mol. This low cost and easy-operated small scale system is useful in chemical, biomedical and environmental labs for quantitative bio-chemical concentration detection with results reported comparable to a highly cost commercial spectrometer.
NASA Technical Reports Server (NTRS)
Brow, Chirstopher; Subramaniam, Ajit; Culver, Mary; Brock, John C.
2000-01-01
Monitoring the health of U.S. coastal waters is an important goal of the National Oceanic and Atmospheric Administration (NOAA). Satellite sensors are capable of providing daily synoptic data of large expanses of the U.S. coast. Ocean color sensor, in particular, can be used to monitor the water quality of coastal waters on an operational basis. To appraise the validity of satellite-derived measurements, such as chlorophyll concentration, the bio-optical algorithms used to derive them must be evaluated in coastal environments. Towards this purpose, over 21 cruises in diverse U.S. coastal waters have been conducted. Of these 21 cruises, 12 have been performed in conjunction with and under the auspices of the NASA/SIMBIOS Project. The primary goal of these cruises has been to obtain in-situ measurements of downwelling irradiance, upwelling radiance, and chlorophyll concentrations in order to evaluate bio-optical algorithms that estimate chlorophyll concentration. In this Technical Memorandum, we evaluate the ability of five bio-optical algorithms, including the current SeaWiFS algorithm, to estimate chlorophyll concentration in surface waters of the South Atlantic Bight (SAB). The SAB consists of a variety of environments including coastal and continental shelf regimes, Gulf Stream waters, and the Sargasso Sea. The biological and optical characteristics of the region is complicated by temporal and spatial variability in phytoplankton composition, primary productivity, and the concentrations of colored dissolved organic matter (CDOM) and suspended sediment. As such, the SAB is an ideal location to test the robustness of algorithms for coastal use.
Characterisation of Vanilla—A novel active pixel sensor for radiation detection
NASA Astrophysics Data System (ADS)
Blue, A.; Bates, R.; Laing, A.; Maneuski, D.; O'Shea, V.; Clark, A.; Prydderch, M.; Turchetta, R.; Arvanitis, C.; Bohndiek, S.
2007-10-01
Novel features of a new monolithic active pixel sensor, Vanilla, with 520×520 pixels ( 25 μm square) has been characterised for the first time. Optimisation of the sensor operation was made through variation of frame rates, integration times and on-chip biases and voltages. Features such as flushed reset operation, ROI capturing and readout modes have been fully tested. Stability measurements were performed to test its suitablility for long-term applications. These results suggest the Vanilla sensor—along with bio-medical and space applications—is suitable for use in particle physics experiments.
A ph sensor based on a flexible substrate
NASA Astrophysics Data System (ADS)
Huang, Wen-Ding
pH sensor is an essential component used in many chemical, food, and bio-material industries. Conventional glass electrodes have been used to construct pH sensors, however, have some disadvantages. Glass electrodes are easily affected by alkaline or HF solution, they require a high input impedance pH meter, they often exhibit a sluggish response. In some specific applications, it is also difficult to use glass electrodes for in vivo biomedical or food monitoring applications due to the difficulty of size miniaturization, planarization and polymerization based on current manufacturing technologies. In this work, we have demonstrated a novel flexible pH sensor based on low-cost sol-gel fabrication process of iridium oxide (IrOx) sensing film (IROF). A pair of flexible miniature IrOx/AgCl electrode generated the action potential from the solution by electrochemical mechanism to obtain the pH level of the reagent. The fabrication process including sol-gel, thermal oxidation, and the electro-plating process of the silver chloride (AgCl) reference electrode were reported in the work. The IrOx film was verified and characterized using electron dispersive analysis (EDAX), scanning electron microscope (SEM), and x-ray diffraction (XRD). The flexible pH sensor's performance and characterization have been investigated with different testing parameters such as sensitivity, response time, stability, reversibility, repeatability, selectivity and temperature dependence. The flexible IrOx pH sensors exhibited promising sensing performance with a near-Nernstian response of sensitivity which is between --51.1mV/pH and --51.7mV/pH in different pH levels ranging from 1.5 to 12 at 25°C. Two applications including gastroesophageal reflux disease (GERD) diagnosis and food freshness wireless monitoring using our micro-flexible IrOx pH sensors were demonstrated. For the GERD diagnosing system, we embedded the micro flexible pH sensor on a 1.2cmx3.8cm of the capsule size of wireless sensor
Bio-Optical Measurement and Modeling of the California Current and Polar Oceans. Chapter 13
NASA Technical Reports Server (NTRS)
Mitchell, B. Greg
2001-01-01
This Sensor Intercomparison and Merger for Biological and Interdisciplinary Oceanic Studies (SIMBIOS) project contract supports in situ ocean optical observations in the California Current, Southern Ocean, Indian Ocean as well as merger of other in situ data sets we have collected on various global cruises supported by separate grants or contracts. The principal goals of our research are to validate standard or experimental products through detailed bio-optical and biogeochemical measurements, and to combine ocean optical observations with advanced radiative transfer modeling to contribute to satellite vicarious radiometric calibration and advanced algorithm development. In collaboration with major oceanographic ship-based observation programs funded by various agencies (CalCOFI, US JGOFS, NOAA AMLR, INDOEX and Japan/East Sea) our SIMBIOS effort has resulted in data from diverse bio-optical provinces. For these global deployments we generate a high-quality, methodologically consistent, data set encompassing a wide-range of oceanic conditions. Global data collected in recent years have been integrated with our on-going CalCOFI database and have been used to evaluate Sea-Viewing Wide Field-of-view Sensor (SeaWiFS) algorithms and to carry out validation studies. The combined database we have assembled now comprises more than 700 stations and includes observations for the clearest oligotrophic waters, highly eutrophic blooms, red-tides and coastal case two conditions. The data has been used to validate water-leaving radiance estimated with SeaWiFS as well as bio optical algorithms for chlorophyll pigments. The comprehensive data is utilized for development of experimental algorithms (e.g., high-low latitude pigment transition, phytoplankton absorption, and cDOM).
Gabl, R; Feucht, H-D; Zeininger, H; Eckstein, G; Schreiter, M; Primig, R; Pitzer, D; Wersing, W
2004-01-15
A novel integrated bio-sensor technology based on thin-film bulk acoustic wave resonators on silicon is presented and the feasibility of detecting DNA and protein molecules proofed. The detection principle of these sensors is label-free and relies on a resonance frequency shift caused by mass loading of an acoustic resonator, a principle very well known from quartz crystal micro balances. Integrated ZnO bulk acoustic wave resonators with resonance frequencies around 2 GHz have been fabricated, employing an acoustic mirror for isolation from the silicon substrate. DNA oligos have been thiol-coupled to the gold electrode by on-wafer dispensing. In a further step, samples have either been hybridised or alternatively a protein has been coupled to the receptor. The measurement results show the new bio-sensor being capable of both, detecting proteins as well as the DNA hybridisation without using a label. Due to the substantially higher oscillation frequency, these sensors already show much higher sensitivity and resolution comparable to quartz crystal micro balances. The potential for these sensors and sensors arrays as well as technological challenges will be discussed in detail.
NASA Astrophysics Data System (ADS)
Estapa, M. L.
2016-02-01
Autonomous, bio-optical profiling floats are poised to broaden the number and spatiotemporal resolution of observations of the ocean's biological pump. Here, we used multiple optical sensors aboard two bio-optical profiling floats (Navis BGCi, Sea-Bird) deployed in the Sargasso Sea to derive in situ proxies for particulate carbon (PC) flux, sub-mixed layer net community production (NCP) and to drive a model of net primary production (NPP). Profiles were collected at approximately 2-day resolution, and drift-phase PC flux observations were collected at subdaily resolution at a rotating cycle of observation depths between 150 and 1000 m. The magnitudes of NPP, PC flux, and their annually-averaged ratio were generally consistent with observations at the nearby Bermuda Atlantic Timeseries Study (BATS) site. PC flux and the export ratio were enhanced in the autumn as well as in the spring, and varied over short timescales possibly due to the influence of mesoscale eddies. The relatively shallow park depths and short profile cycle lengths allow us to identify ephemeral, subsurface bio-optical features and compare them to measured fluxes and satellite-observed surface properties.
USDA-ARS?s Scientific Manuscript database
The use of field effect transistors (FETs) as the transduction element for the detection of DNA amplification reactions will enable portable and inexpensive nucleic acid analysis. Transistors used as biological sensors,or BioFETs, minimize the cost and size of detection platforms by leveraging fabri...
NASA Astrophysics Data System (ADS)
Piron, P.; Vargas Catalan, E.; Haas, J.; Österlund, L.; Nikolajeff, F.; Andersson, P. O.; Bergström, J.; Mizaikoff, B.; Karlsson, M.
2018-02-01
Microfabricated diamond waveguides, between 5 and 20 μm thick, manufactured by chemical vapor deposition of diamond, followed by standard lithographic techniques and inductively coupled plasma etching of diamond, are used as bio-chemical sensors in the mid infrared domain: 5-11 μm. Infrared light, emitted from a broadly tunable quantum cascade laser with a wavelength resolution smaller than 20 nm, is coupled through the diamond waveguides for attenuated total reflection spectroscopy. The expected advantages of these waveguides are a high sensitivity due to the high number of internal reflections along the propagation direction, a high transmittance in the mid-IR domain, the bio-compatibility of diamond and the possibility of functionalizing the surface layer. The sensor will be used for analyzing different forms of proteins such as α-synuclein which is relevant in understanding the mechanism behind Parkinson's disease. The fabrication process of the waveguide, its characteristics and several geometries are introduced. The optical setup of the biosensor is described and our first measurements on two analytes to demonstrate the principle of the sensing method will be presented. Future use of this sensor includes the functionalization of the diamond waveguide sensor surface to be able to fish out alpha-synuclein from cerebrospinal fluid.
Micro packaged MEMS pressure sensor for intracranial pressure measurement
NASA Astrophysics Data System (ADS)
Xiong, Liu; Yan, Yao; Jiahao, Ma; Yanhang, Zhang; Qian, Wang; Zhaohua, Zhang; Tianling, Ren
2015-06-01
This paper presents a micro packaged MEMS pressure sensor for intracranial pressure measurement which belongs to BioMEMS. It can be used in lumbar puncture surgery to measure intracranial pressure. Miniaturization is key for lumbar puncture surgery because the sensor must be small enough to allow it be placed in the reagent chamber of the lumbar puncture needle. The size of the sensor is decided by the size of the sensor chip and package. Our sensor chip is based on silicon piezoresistive effect and the size is 400 × 400 μm2. It is much smaller than the reported polymer intracranial pressure sensors such as liquid crystal polymer sensors. In terms of package, the traditional dual in-line package obviously could not match the size need, the minimal size of recently reported MEMS-based intracranial pressure sensors after packaging is 10 × 10 mm2. In this work, we are the first to introduce a quad flat no-lead package as the package form of piezoresistive intracranial pressure sensors, the whole size of the sensor is minimized to only 3 × 3 mm2. Considering the liquid measurement environment, the sensor is gummed and waterproof performance is tested; the sensitivity of the sensor is 0.9 × 10-2 mV/kPa. Project supported by the National Natural Science Foundation of China (Nos. 61025021, 61434001), and the ‘Thousands Talents’ Program for Pioneer Researchers and Its Innovation Team, China.
NASA Technical Reports Server (NTRS)
Mueller, James L.
2001-01-01
This Sensor Intercomparison and Merger for Biological and Interdisciplinary Oceanic Studies (SIMBIOS) contract supports acquisition of match up radiometric and bio-optical data for validation of Sea-Viewing Wide Field-of-view Sensor (SeaWiFS) and other ocean color satellites, and evaluation of uncertainty budgets and protocols for in situ measurements of normalized water leaving radiances.
NASA Astrophysics Data System (ADS)
O'Toole, M. D.; Marsh, L. A.; Davidson, J. L.; Tan, Y. M.; Armitage, D. W.; Peyton, A. J.
2015-03-01
Biological tissues have a complex impedance, or bio-impedance, profile which changes with respect to frequency. This is caused by dispersion mechanisms which govern how the electromagnetic field interacts with the tissue at the cellular and molecular level. Measuring the bio-impedance spectra of a biological sample can potentially provide insight into the sample’s properties and its cellular structure. This has obvious applications in the medical, pharmaceutical and food-based industrial domains. However, measuring the bio-impedance spectra non-destructively and in a way which is practical at an industrial scale presents substantial challenges. The low conductivity of the sample requires a highly sensitive instrument, while the demands of industrial-scale operation require a fast high-throughput sensor of rugged design. In this paper, we describe a multi-frequency magnetic induction spectroscopy (MIS) system suitable for industrial-scale, non-contact, spectroscopic bio-impedance measurement over a bandwidth of 156 kHz-2.5 MHz. The system sensitivity and performance are investigated using calibration and known reference samples. It is shown to yield rapid and consistently sensitive results with good long-term stability. The system is then used to obtain conductivity spectra of a number of biological test samples, including yeast suspensions of varying concentration and a range of agricultural produce, such as apples, pears, nectarines, kiwis, potatoes, oranges and tomatoes.
Determining the bio-based content of bio-plastics used in Thailand by radiocarbon analysis
NASA Astrophysics Data System (ADS)
Ploykrathok, T.; Chanyotha, S.
2017-06-01
Presently, there is an increased interest in the development of bio-plastic products from agricultural materials which are biodegradable in order to reduce the problem of waste disposal. Since the amount of modern carbon in bio-plastics can indicate how much the amount of agricultural materials are contained in the bio-plastic products, this research aims to determine the modern carbon in bio-plastic using the carbon dioxide absorption method. The radioactivity of carbon-14 contained in the sample is measured by liquid scintillation counter (Tri-carb 3110 TR, PerkinElmer). The percentages of bio-based content in the samples were determined by comparing the observed modern carbon content with the values contained in agricultural raw materials. The experimental results show that only poly(lactic acid) samples have the modern carbon content of 97.4%, which is close to the agricultural materials while other bio-plastics types are found to have less than 50% of the modern carbon content. In other words, most of these bio-plastic samples were mixed with other materials which are not agriculturally originated.
Pyrolysis of hornbeam (Carpinus betulus L.) sawdust: Characterization of bio-oil and bio-char.
Moralı, Uğur; Yavuzel, Nazan; Şensöz, Sevgi
2016-12-01
Slow pyrolysis of hornbeam (Carpinus betulus L.) sawdust was performed to produce bio-oil and bio-char. The operational variables were as follows: pyrolysis temperature (400-600°C), heating rate (10-50°Cmin -1 ) and nitrogen flow rate (50-150cm 3 min -1 ). Physicochemical and thermogravimetric characterizations of hornbeam sawdust were performed. The characteristics of bio-oil and bio-char were analyzed on the basis of various spectroscopic and chromatographic techniques such as FTIR, GC-MS, 1H NMR, SEM, BET. Higher heating value, density and kinematic viscosity of the bio-oil with maximum yield of 35.28% were 23.22MJkg -1 , 1289kgm -3 and 0.6mm 2 s -1 , respectively. The bio-oil with relatively high fuel potential can be obtained from the pyrolysis of the hornbeam sawdust and the bio-char with a calorific value of 32.88MJkg -1 is a promising candidate for solid fuel applications that also contributes to the preservation of the environment. Copyright © 2016 Elsevier Ltd. All rights reserved.
NASA Astrophysics Data System (ADS)
Organelli, E.; Claustre, H.; Serra, R.; Bricaud, A.; Schmechtig, C.; D'Ortenzio, F.; Poteau, A.; Mangin, A.; Leymarie, E.; Obolensky, G.; Prieur, L. M.; Dall'Olmo, G.; Xing, X.
2016-02-01
Thanks to a new generation of Bio-Argo floats equipped with sensors for PAR (Photosynthetically Available Irradiance) and downward irradiance measurements at selected wavelengths (i.e., 380, 412 and 490 nm), the number of radiometric measurements has been dramatically increasing and data are available for diverse open ocean systems, including winter periods with harsh seas when ships can hardly sample. More than 6500 radiometric profiles have so far been acquired around solar noon in the upper 250 m of the ocean. These radiometric profiles, acquired simultaneously to other key biogeochemical and bio-optical variables (chlorophyll a, CDOM, light backscattering), represent a fruitful data source for validation of Ocean Color (OC) products. Two different strategies can be implemented: direct validation of satellite OC products and identification of regions characterized by bio-optical anomalies. Diffuse attenuation coefficients (Kd) derived from these profiles, after a specifically developed quality control, are used for these purposes.A good agreement is observed between satellite-derived Kd values at 490 nm and their Bio-Argo counterparts. However, satellite overestimates low in situ Kd values found in very clear waters (e.g., Atlantic and Pacific Sub-Tropical Gyres). The analysis of the spectral Kd variability in the surface ocean shows the potential of Bio-Argo floats in identifying those regions with optical properties departing from global bio-optical relationships. Divergences of the ratio between Kd values at 380 nm and those at 490 nm from global bio-optical models are observed in areas such as the Mediterranean Sea and the North Atlantic in winter. This might cause difficulties in retrieving biogeochemical parameters from satellite data. Hence, delineation of "anomalous" regions by Bio-Argo floats represents a useful strategy for planning dedicated cruises, setting mooring buoys or using CAL/VAL floats in order to improve Ocean Color applications.
Silicon sensors for catheters and guide wires
NASA Astrophysics Data System (ADS)
Goosen, Hans F.
2001-11-01
One area that can make use of the miniature size of present day micro electromechanical systems (MEMS) is that of the medical field of minimally invasive interventions. These procedures, used for both diagnosis and treatment, use catheters that are advanced through the blood vessels deep into the body, without the need for surgery. However, once inside the body, the doctor performing the procedure is completely reliant on the information the catheter(s) can provide in addition to the projection imaging of a fluoroscope. A good range of sensors for catheters is required for a proper diagnosis. To this end, miniature sensors are being developed to be fitted to catheters and guide wires. As the accurate positioning of these instruments is problematic, it is necessary to combine several sensors on the same guide wire or catheter to measure several parameters in the same location. This however, brings many special problems to the design of the sensors, such as small size, low power consumption, bio-compatibility of materials, robust design for patient safety, a limited number of connections, packaging, etc. This paper will go into both the advantages and design problems of micromachined sensors and actuators in catheters and guide wires. As an example, a multi parameter blood sensor, measuring flow velocity, pressure and oxygen saturation, will be discussed.
NASA Technical Reports Server (NTRS)
Mueller, J. L.; Fargion, G. S.; McClain, C. R. (Editor); Pegau, S.; Zanefeld, J. R. V.; Mitchell, B. G.; Kahru, M.; Wieland, J.; Stramska, M.
2003-01-01
This document stipulates protocols for measuring bio-optical and radiometric data for the Sensor Intercomparision and Merger for Biological and Interdisciplinary Oceanic Studies (SIMBIOS) Project activities and algorithm development. The document is organized into 6 separate volumes as Ocean Optics Protocols for Satellite Ocean Color Sensor Validation, Revision 4. Volume I: Introduction, Background, and Conventions; Volume II: Instrument Specifications, Characterization and Calibration; Volume III: Radiometric Measurements and Data Analysis Methods; Volume IV: Inherent Optical Properties: Instruments, Characterization, Field Measurements and Data Analysis Protocols; Volume V: Biogeochemical and Bio-Optical Measurements and Data Analysis Methods; Volume VI: Special Topics in Ocean Optics Protocols and Appendices. The earlier version of Ocean Optics Protocols for Satellite Ocean Color Sensor Validation, Revision 3 is entirely superseded by the six volumes of Revision 4 listed above.
Elman, Noel M; Ben-Yoav, Hadar; Sternheim, Marek; Rosen, Rachel; Krylov, Slava; Shacham-Diamand, Yosi
2008-06-15
A lab-on-chip consisting of a unique integration of whole-cell sensors, a MOEMS (Micro-Opto-Electro-Mechanical-System) modulator, and solid-state photo-detectors was implemented for the first time. Whole-cell sensors were genetically engineered to express a bioluminescent reporter (lux) as a function of the lac promoter. The MOEMS modulator was designed to overcome the inherent low frequency noise of solid-state photo-detectors by means of a previously reported modulation technique, named IHOS (Integrated Heterodyne Optical System). The bio-reporter signals were modulated prior to photo-detection, increasing the SNR of solid-state photo-detectors at least by three orders of magnitude. Experiments were performed using isopropyl-beta-d-thiogalactopyranoside (IPTG) as a preliminary step towards testing environmental toxicity. The inducer was used to trigger the expression response of the whole-cell sensors testing the sensitivity of the lab-on-chip. Low intensity bio-reporter optical signals were measured after the whole-cell sensors were exposed to IPTG concentrations of 0.1, 0.05, and 0.02mM. The experimental results reveal the potential of this technology for future implementation as an inexpensive massive method for rapid environmental toxicity detection.
NASA Astrophysics Data System (ADS)
Dusek, J.; Kottapalli, A. G. P.; Woo, M. E.; Asadnia, M.; Miao, J.; Lang, J. H.; Triantafyllou, M. S.
2013-01-01
The lateral line found on most species of fish is a sensory organ without analog in humans. Using sensory feedback from the lateral line, fish are able to track prey, school, avoid obstacles, and detect vortical flow structures. Composed of both a superficial component, and a component contained within canals beneath the fish’s skin, the lateral line acts in a similar fashion to an array of differential pressure sensors. In an effort to enhance the situational and environmental awareness of marine vehicles, lateral-line-inspired pressure sensor arrays were developed to mimic the enhanced sensory capabilities observed in fish. Three flexible and waterproof pressure sensor arrays were fabricated for use as a surface-mounted ‘smart skin’ on marine vehicles. Two of the sensor arrays were based around the use of commercially available piezoresistive sensor dies, with innovative packaging schemes to allow for flexibility and underwater operation. The sensor arrays employed liquid crystal polymer and flexible printed circuit board substrates with metallic circuits and silicone encapsulation. The third sensor array employed a novel nanocomposite material set that allowed for the fabrication of a completely flexible sensor array. All three sensors were surface mounted on the curved hull of an autonomous kayak vehicle, and tested in both pool and reservoir environments. Results demonstrated that all three sensors were operational while deployed on the autonomous vehicle, and provided an accurate means for monitoring the vehicle dynamics.
Tailor-made resealable micro(bio)reactors providing easy integration of in situ sensors
NASA Astrophysics Data System (ADS)
Viefhues, Martina; Sun, Shiwen; Valikhani, Donya; Nidetzky, Bernd; Vrouwe, Elwin X.; Mayr, Torsten; Bolivar, Juan M.
2017-06-01
Flow microreactors utilizing immobilized enzymes are of great interest in biocatalysis development. Most of the common devices are permanently closed, single-use systems, which allow limited physical and chemical surface modifications and evaluation methods. In this paper we will present resealable flowcells that overcome these limitations and moreover allow a quick and easy integration of sensor systems, because of the use of modular building blocks. The devices were utilized to study the enzyme activity of glucose oxidase immobilized on chemically modified glass surfaces under flow conditions, employing integrated optical oxygen sensors for on-line monitoring.
NASA Astrophysics Data System (ADS)
Lee, El-Hang; Lee, Hyun S.; Lee, S. G.; O, B. H.; Park, S. G.; Kim, K. H.
2007-05-01
We report on the design of micro-ring resonator optical sensors for integration on what we call optical printed circuit boards (O-PCBs). The objective is to realize application-specific O-PCBs, either on hard board or on flexible board, by integrating micro/nano-scale optical sensors for compact, light-weight, low-energy, high-speed, intelligent, and environmentally friendly processing of information. The O-PCBs consist of two-dimensional planar arrays of micro/nano-scale optical wires, circuits and devices that are interconnected and integrated to perform the functions of sensing and then storing, transporting, processing, switching, routing and distributing optical signals that have been collected by means of sensors. For fabrication, the polymer and organic optical wires and waveguides are first fabricated on a board and are used to interconnect and integrate sensors and other micro/ nano-scale photonic devices. Here, in our study, we focus on the sensors based on the micro-ring structures. We designed bio-sensors using silicon based micro-ring resonator. We investigate the characteristics such as sensitivity and selectivity (or quality factor) of micro-ring resonator for their use in bio-sensing application. We performed simulation studies on the quality factor of micro-ring resonators by varying the radius of the ring resonators and the separation between adjacent waveguides. We introduce the effective coupling coefficient as a realistic value to describe the strength of the coupling in micro-ring resonators.
The problem of bio-concepts: biopolitics, bio-economy and the political economy of nothing
NASA Astrophysics Data System (ADS)
Birch, Kean
2017-12-01
Scholars in science and technology studies—and no doubt other fields—have increasingly drawn on Michel Foucault's concept of biopolitics to theorize a variety of new `bio-concepts'. While there might be some theoretical value in such exercises, many of these bio-concepts have simply replaced more rigorous—and therefore time-consuming—analytical work. This article provides a (sympathetic) critique of these various bio-concepts, especially as they are applied to the emerging `bio-economy'. In so doing, the article seeks to show that the analysis of the bio-economy could be better framed as a political economy of nothing. This has several implications for science education, which are raised in the article.
Method to upgrade bio-oils to fuel and bio-crude
Steele, Philip H; Pittman, Jr., Charles U; Ingram, Jr., Leonard L; Gajjela, Sanjeev; Zhang, Zhijun; Bhattacharya, Priyanka
2013-12-10
This invention relates to a method and device to produce esterified, olefinated/esterified, or thermochemolytic reacted bio-oils as fuels. The olefinated/esterified product may be utilized as a biocrude for input to a refinery, either alone or in combination with petroleum crude oils. The bio-oil esterification reaction is catalyzed by addition of alcohol and acid catalyst. The olefination/esterification reaction is catalyzed by addition of resin acid or other heterogeneous catalyst to catalyze olefins added to previously etherified bio-oil; the olefins and alcohol may also be simultaneously combined and catalyzed by addition of resin acid or other heterogeneous catalyst to produce the olefinated/esterified product.
NASA Technical Reports Server (NTRS)
Mueller, J. L. (Editor); Fargion, Giuletta S. (Editor); McClain, Charles R. (Editor); Pegau, Scott; Zaneveld, J. Ronald V.; Mitchell, B. Gregg; Kahru, Mati; Wieland, John; Stramska, Malgorzat
2003-01-01
This document stipulates protocols for measuring bio-optical and radiometric data for the Sensor Intercomparison and Merger for Biological and Interdisciplinary Oceanic Studies (SIMBIOS) Project activities and algorithm development. The document is organized into 6 separate volumes as Ocean Optics Protocols for Satellite Ocean Color Sensor Validation, Revision 4. Volume I: Introduction, Background and Conventions; Volume II: Instrument Specifications, Characterization and Calibration; Volume III: Radiometric Measurements and Data Analysis Methods; Volume IV: Inherent Optical Properties: Instruments, Characterization, Field Measurements and Data Analysis Protocols; Volume V: Biogeochemical and Bio-Optical Measurements and Data Analysis Methods; Volume VI: Special Topics in Ocean Optics Protocols and Appendices. The earlier version of Ocean Optics Protocols for Satellite Ocean Color Sensor Validation, Revision 3 (Mueller and Fargion 2002, Volumes 1 and 2) is entirely superseded by the six volumes of Revision 4 listed above.
Evaluation of Fuzzy Fiber Sensors for Structural Health Monitoring
2010-11-01
detect damage. Should damage occur at other unanticipated regions, it may go undetected. Methods have been devised to use the sensors in a network...graphene tubes around the core of an SWCNT lead to multi-walled carbon nanotubes ( MWCNTs ). These CNTs have diameters in a range between one to tens...performance carbon-carbon composites, EMI shielding, lightning strike, energy storage, thermal management, bio-implants, and bone regeneration (pend- ing
Recent Advances in Skin-Inspired Sensors Enabled by Nanotechnology
NASA Astrophysics Data System (ADS)
Loh, Kenneth J.; Azhari, Faezeh
2012-07-01
The highly optimized performance of nature's creations and biological assemblies has inspired the development of their bio-inspired artificial counterparts that can potentially outperform conventional systems. In particular, the skin of humans, animals, and insects exhibits unique functionalities and properties and has subsequently led to active research in developing skin-inspired sensors. This paper presents a summary of selected work related to skin-inspired tactile, distributed strain, and artificial hair cell flow sensors, with a particular focus on technologies enabled by recent advancements in the nanotechnology domain. The purpose is not to present a comprehensive review on this broad subject matter but rather to use selected work to outline the diversity of current research activities.
NASA Astrophysics Data System (ADS)
Cowles, T. J.; Barth, J. A.; Wingard, C. E.; Desiderio, R. A.; Letelier, R. M.; Pierce, S. D.
2002-12-01
Mesoscale mapping of the hydrographic and bio-optical properties of the Northern California Current System was conducted during spring and summer 2000, 2001, and 2002 off the Oregon coast. A towed, undulating vehicle carried a CTD, two fluorometers, a multi-wavelength absorption and attenuation meter (ac-9), and a PAR sensor. In addition, an ac-9 and a Fast Repetition Rate fluorometer (FRRf) collected bio-optical data on surface waters throughout the mesoscale surveys. Multiple onshore-offshore transect lines provided repeated crossings of velocity jet and frontal boundaries, and allowed resolution of physical and bio-optical parameters on horizontal scales of 1km or less and on vertical scales of 1-2m. Our multi-year results permit assessment of the linkages and the degree of coupling between physical and bio-optical patterns during strong upwelling and strong downwelling events, as well as during low-wind relaxation intervals. The location of the coastal jet and the upwelling front fluctuated considerably under the variable forcing regime, with more extensive mesoscale structure in all parameters in late summer relative to spring, as current meanders developed around subsurface topography (Heceta Bank) and moved offshore near Cape Blanco. Sharp horizontal gradients in autotrophic biomass were observed across the boundaries of the coastal jet and the upwelling front, with chlorophyll levels often in excess of 5-10 mg m-3 on the inshore side of the fronts. Horizontal gradients also were observed in the spectral slope of attenuation and dissolved absorption as well as in the physiological properties of the autotrophic assemblages (as determined with FRRf). Details of the spatial correlations of physical and bio-optical parameters will be presented.
Migrating the Mach-Zehnder chemical and bio-sensor to the mid-infrared region
NASA Astrophysics Data System (ADS)
Leidner, L.; Ewald, M.; Sieger, M.; Mizaikoff, B.; Gauglitz, G.
2013-05-01
The properties of integrated optical phase-modulated Mach-Zehnder interferometers (IO-MZI) are used to set up a new generation of chemical and biochemical sensors working in the mid-infrared. First applications of the MZI principle were introduced in the beginning 1990s. They range from a gas sensor to monitor organic solvent concentrations1 to setting up an immunoassay for the detection of the herbicide simazine2. Most if not all sensors of MZI type operate at wavelengths of the visible or near infrared spectrum. There are several reasons to change this strategy and move into the mid-infrared spectral range (MIR): higher manufacturing tolerances, increased evanescent field penetration depth, signal amplification by surface enhanced infrared absorption effect (SEIRA), species identification by MIR fingerprints. The basis of the planned MIR-MZI is a GaAs waveguide pattern epitaxially grown on a substrate3. As a first step towards nanostructuring the waveguide surface, chemical deposition of Au nanoparticles on GaAs transducers was established. For the use of MIR-MZI sensors in bioanalytical assay development, chemical immobilization of molecular recognition elements on GaAs transducers was carried out. The modified surfaces were characterized by atomic force microscopy (AFM), dark field microscopy, contact angle measurements and ellipsometric data as well as by a modified version of Reflectometric Interference Spectroscopy (RIfS)4. It was possible to monitor both the immobilization of gold nanoparticles and time-resolved specific binding using a model antibody antigen assay. After successful setup of relevant assays with RIfS, e.g. the detection of bacteria or endocrine disruptors, the assays are designed to be transferred onto the mid-infrared Mach-Zehnder interferometer.
DOE Office of Scientific and Technical Information (OSTI.GOV)
Ben Hassen-Trabelsi, A., E-mail: aidabenhassen@yahoo.fr; Kraiem, T.; Département de Géologie, Université de Tunis, 2092, Tunis
Highlights: • Produced bio-fuels (bio-oil and bio-char) from some animal fatty wastes. • Investigated the effects of main parameters on pyrolysis products distribution. • Determined the suitable conditions for the production of the maximum of bio-oil. • Characterized bio-oils and bio-chars obtained from several animal fatty wastes. - Abstract: Several animal (lamb, poultry and swine) fatty wastes were pyrolyzed under nitrogen, in a laboratory scale fixed-bed reactor and the main products (liquid bio-oil, solid bio-char and syngas) were obtained. The purpose of this study is to produce and characterize bio-oil and bio-char obtained from pyrolysis of animal fatty wastes. Themore » maximum production of bio-oil was achieved at a pyrolysis temperature of 500 °C and a heating rate of 5 °C/min. The chemical (GC–MS analyses) and spectroscopic analyses (FTIR analyses) of bio-oil showed that it is a complex mixture consisting of different classes of organic compounds, i.e., hydrocarbons (alkanes, alkenes, cyclic compounds…etc.), carboxylic acids, aldehydes, ketones, esters,…etc. According to fuel properties, produced bio-oils showed good properties, suitable for its use as an engine fuel or as a potential source for synthetic fuels and chemical feedstock. Obtained bio-chars had low carbon content and high ash content which make them unattractive for as renewable source energy.« less
Quantum Sensing Beyond the Shot-Noise Limit with Plasmonic Sensors
NASA Astrophysics Data System (ADS)
Dowran, Mohammadjavad; Kumar, Ashok; Lawrie, Benjamin; Pooser, Raphael; Marino, Alberto
2017-04-01
The use of quantum resources offers the possibility of enhancing the sensitivity of a device beyond the shot noise limit and promises to revolutionize the field of metrology through the development of quantum enhanced sensors. In particular, plasmonic sensors, which are widely used in bio-chemical sensing applications, provide a unique opportunity to bring such an enhancement to real-life devices. Resonance plasmonic sensors respond to changes in refractive index through a shift of their characteristic transmission spectrum. We show that the use of quantum squeezed states to probe plasmonic sensors can enhance their sensitivity by lowering the noise floor and allowing the detection of smaller changes in refractive index. In our experiment, we use one of the beams of a two-mode squeezed state generated via four-wave-mixing in Rb atoms to probe the sensor. A squeezing level of 4 dB is obtained after transduction through the plasmonic sensor, which consists of a triangular nano-hole array in a thin silver film and exhibits a sensitivity of the order of 10-10 RIU /√{ Hz} . The use of quantum states leads to 40 % enhancement in the sensitivity of the plasmonic sensor with respect to the shot noise limit. Work supported by the W.M. Keck Foundation.
Navigating the Bio-Politics of Childhood
ERIC Educational Resources Information Center
Lee, Nick; Motzkau, Johanna
2011-01-01
Childhood research has long shared a bio-political terrain with state agencies in which children figure primarily as "human futures". In the 20th century bio-social dualism helped to make that terrain navigable by researchers, but, as life processes increasingly become key sites of bio-political action, bio-social dualism is becoming…
Biogeochemical sensor performance in the SOCCOM profiling float array
NASA Astrophysics Data System (ADS)
Johnson, Kenneth S.; Plant, Joshua N.; Coletti, Luke J.; Jannasch, Hans W.; Sakamoto, Carole M.; Riser, Stephen C.; Swift, Dana D.; Williams, Nancy L.; Boss, Emmanuel; Haëntjens, Nils; Talley, Lynne D.; Sarmiento, Jorge L.
2017-08-01
The Southern Ocean Carbon and Climate Observations and Modeling (SOCCOM) program has begun deploying a large array of biogeochemical sensors on profiling floats in the Southern Ocean. As of February 2016, 86 floats have been deployed. Here the focus is on 56 floats with quality-controlled and adjusted data that have been in the water at least 6 months. The floats carry oxygen, nitrate, pH, chlorophyll fluorescence, and optical backscatter sensors. The raw data generated by these sensors can suffer from inaccurate initial calibrations and from sensor drift over time. Procedures to correct the data are defined. The initial accuracy of the adjusted concentrations is assessed by comparing the corrected data to laboratory measurements made on samples collected by a hydrographic cast with a rosette sampler at the float deployment station. The long-term accuracy of the corrected data is compared to the GLODAPv2 data set whenever a float made a profile within 20 km of a GLODAPv2 station. Based on these assessments, the fleet average oxygen data are accurate to 1 ± 1%, nitrate to within 0.5 ± 0.5 µmol kg-1, and pH to 0.005 ± 0.007, where the error limit is 1 standard deviation of the fleet data. The bio-optical measurements of chlorophyll fluorescence and optical backscatter are used to estimate chlorophyll a and particulate organic carbon concentration. The particulate organic carbon concentrations inferred from optical backscatter appear accurate to with 35 mg C m-3 or 20%, whichever is larger. Factors affecting the accuracy of the estimated chlorophyll a concentrations are evaluated.
2007-07-01
testfaciliteit detector met de drie simulanten getest beschikbaar gekomen voor het testen van Beschrijving van de worden volgens gangbare internationale bio...2005 werd de Bio-Adrosol Testkamer (BAT-kamer) geplaatst door de firma Dycor Technologies Ltd., Canada. In de BAT-kamner kan een bio-ai5rosol...bestaande ruimte past. In beide gevallen bleek de firma Dycor Technologies Ltd in Canada (http://www.dycor.com) de beste leverancier te zijn, en voor beide
BioNetFit: a fitting tool compatible with BioNetGen, NFsim and distributed computing environments
Thomas, Brandon R.; Chylek, Lily A.; Colvin, Joshua; Sirimulla, Suman; Clayton, Andrew H.A.; Hlavacek, William S.; Posner, Richard G.
2016-01-01
Summary: Rule-based models are analyzed with specialized simulators, such as those provided by the BioNetGen and NFsim open-source software packages. Here, we present BioNetFit, a general-purpose fitting tool that is compatible with BioNetGen and NFsim. BioNetFit is designed to take advantage of distributed computing resources. This feature facilitates fitting (i.e. optimization of parameter values for consistency with data) when simulations are computationally expensive. Availability and implementation: BioNetFit can be used on stand-alone Mac, Windows/Cygwin, and Linux platforms and on Linux-based clusters running SLURM, Torque/PBS, or SGE. The BioNetFit source code (Perl) is freely available (http://bionetfit.nau.edu). Supplementary information: Supplementary data are available at Bioinformatics online. Contact: bionetgen.help@gmail.com PMID:26556387
Alahnomi, Rammah A; Zakaria, Z; Ruslan, E; Ab Rashid, S R; Mohd Bahar, Amyrul Azuan; Shaaban, Azizah
2017-01-01
A novel symmetrical split ring resonator (SSRR) based microwave sensor with spurline filters for detecting and characterizing the properties of solid materials has been developed. Due to the weak perturbation in the interaction of material under test (MUT) and planar microwave sensor, spurline filters were embedded to the SSRR microwave sensor which effectively enhanced Q-factor with suppressing the undesired harmonic frequency. The spurline filter structures force the presented sensor to resonate at a fundamental frequency of 2.2 GHz with the capabilities of suppressing rejected harmonic frequency and miniaturization in circuit size. A wide bandwidth rejection is achieved by using double spurlines filters with high Q-factor achievement (up to 652.94) compared to single spurline filter. The new SSRR sensor with spurline filters displayed desired properties such as high sensitivity, accuracy, and performance with a 1.3% typical percentage error in the measurement results. Furthermore, the sensor has been successfully applied for detecting and characterizing solid materials (such as Roger 5880, Roger 4350, and FR4) and evidently demonstrated that it can suppress the harmonic frequency effectively. This novel design with harmonic suppression is useful for various applications such as food industry (meat, fruit, vegetables), biological medicine (derived from proteins and other substances produced by the body), and Therapeutic goods (antiseptics, vitamins, anti-psychotics, and other medicines).
Ruslan, E.; Ab Rashid, S. R.; Mohd Bahar, Amyrul Azuan; Shaaban, Azizah
2017-01-01
A novel symmetrical split ring resonator (SSRR) based microwave sensor with spurline filters for detecting and characterizing the properties of solid materials has been developed. Due to the weak perturbation in the interaction of material under test (MUT) and planar microwave sensor, spurline filters were embedded to the SSRR microwave sensor which effectively enhanced Q-factor with suppressing the undesired harmonic frequency. The spurline filter structures force the presented sensor to resonate at a fundamental frequency of 2.2 GHz with the capabilities of suppressing rejected harmonic frequency and miniaturization in circuit size. A wide bandwidth rejection is achieved by using double spurlines filters with high Q-factor achievement (up to 652.94) compared to single spurline filter. The new SSRR sensor with spurline filters displayed desired properties such as high sensitivity, accuracy, and performance with a 1.3% typical percentage error in the measurement results. Furthermore, the sensor has been successfully applied for detecting and characterizing solid materials (such as Roger 5880, Roger 4350, and FR4) and evidently demonstrated that it can suppress the harmonic frequency effectively. This novel design with harmonic suppression is useful for various applications such as food industry (meat, fruit, vegetables), biological medicine (derived from proteins and other substances produced by the body), and Therapeutic goods (antiseptics, vitamins, anti-psychotics, and other medicines). PMID:28934301
Integration of GMR Sensors with Different Technologies
Cubells-Beltrán, María-Dolores; Reig, Càndid; Madrenas, Jordi; De Marcellis, Andrea; Santos, Joana; Cardoso, Susana; Freitas, Paulo P.
2016-01-01
Less than thirty years after the giant magnetoresistance (GMR) effect was described, GMR sensors are the preferred choice in many applications demanding the measurement of low magnetic fields in small volumes. This rapid deployment from theoretical basis to market and state-of-the-art applications can be explained by the combination of excellent inherent properties with the feasibility of fabrication, allowing the real integration with many other standard technologies. In this paper, we present a review focusing on how this capability of integration has allowed the improvement of the inherent capabilities and, therefore, the range of application of GMR sensors. After briefly describing the phenomenological basis, we deal on the benefits of low temperature deposition techniques regarding the integration of GMR sensors with flexible (plastic) substrates and pre-processed CMOS chips. In this way, the limit of detection can be improved by means of bettering the sensitivity or reducing the noise. We also report on novel fields of application of GMR sensors by the recapitulation of a number of cases of success of their integration with different heterogeneous complementary elements. We finally describe three fully functional systems, two of them in the bio-technology world, as the proof of how the integrability has been instrumental in the meteoric development of GMR sensors and their applications. PMID:27338415
NASA's Ship-Aircraft Bio-Optical Research (SABOR)
2017-12-08
Instruments Overboard On July 26, 2014, scientists worked past dusk to prepare and deploy the optical instruments and ocean water sensors during NASA's SABOR experiment. NASA's Ship-Aircraft Bio-Optical Research (SABOR) experiment is a coordinated ship and aircraft observation campaign off the Atlantic coast of the United States, an effort to advance space-based capabilities for monitoring microscopic plants that form the base of the marine food chain. Read more: 1.usa.gov/WWRVzj Credit: NASA/SABOR/Wayne Slade, Sequoia Scientific . NASA image use policy. NASA Goddard Space Flight Center enables NASA’s mission through four scientific endeavors: Earth Science, Heliophysics, Solar System Exploration, and Astrophysics. Goddard plays a leading role in NASA’s accomplishments by contributing compelling scientific knowledge to advance the Agency’s mission. Follow us on Twitter Like us on Facebook Find us on Instagram
Kim, Sun; Chatr-aryamontri, Andrew; Chang, Christie S.; Oughtred, Rose; Rust, Jennifer; Wilbur, W. John; Comeau, Donald C.; Dolinski, Kara; Tyers, Mike
2017-01-01
A great deal of information on the molecular genetics and biochemistry of model organisms has been reported in the scientific literature. However, this data is typically described in free text form and is not readily amenable to computational analyses. To this end, the BioGRID database systematically curates the biomedical literature for genetic and protein interaction data. This data is provided in a standardized computationally tractable format and includes structured annotation of experimental evidence. BioGRID curation necessarily involves substantial human effort by expert curators who must read each publication to extract the relevant information. Computational text-mining methods offer the potential to augment and accelerate manual curation. To facilitate the development of practical text-mining strategies, a new challenge was organized in BioCreative V for the BioC task, the collaborative Biocurator Assistant Task. This was a non-competitive, cooperative task in which the participants worked together to build BioC-compatible modules into an integrated pipeline to assist BioGRID curators. As an integral part of this task, a test collection of full text articles was developed that contained both biological entity annotations (gene/protein and organism/species) and molecular interaction annotations (protein–protein and genetic interactions (PPIs and GIs)). This collection, which we call the BioC-BioGRID corpus, was annotated by four BioGRID curators over three rounds of annotation and contains 120 full text articles curated in a dataset representing two major model organisms, namely budding yeast and human. The BioC-BioGRID corpus contains annotations for 6409 mentions of genes and their Entrez Gene IDs, 186 mentions of organism names and their NCBI Taxonomy IDs, 1867 mentions of PPIs and 701 annotations of PPI experimental evidence statements, 856 mentions of GIs and 399 annotations of GI evidence statements. The purpose, characteristics and possible future
Wang, Qihui; Li, Bo; Wang, Yuhuai; Shou, Zhouxiang; Shi, Guolong
2015-05-01
A three-dimensional hierarchical CdO nanostructure with a novel bio-inspired morphology is reported. The field emission scanning electronic microscopy, transmission electron microscopy and X-ray diffractometer were employed to characterize the as-prepared samples. In gas-sensing measurements, acetone and diethyl ether were employed as target gases to investigate cataluminescence (CTL) sensing properties of the CdO nanostructure. The results show that the as-fabricated CdO nanostructure exhibited outstanding CTL properties such as stable intensity, high signal/noise values, short response and recovery time. The limit of detection of acetone and diethyl ether was ca. 6.5 ppm and 6.7 ppm, respectively, which was below the standard permitted concentrations. Additionally, a principal components analysis method was used to investigate the recognizable ability of the CTL sensor, and it was found that acetone and diethyl ether can be distinguished clearly. The performance of the bio-inspired CdO nanostructure-based sensor system suggested the promising application of the CdO nanostructure as a novel highly efficient CTL sensing material. Copyright © 2014 John Wiley & Sons, Ltd.
Thammanabhatla, Pratibha; Pailla, Mamatha
2010-08-01
The Bangalore India Bio 2010 conference, held in Bangalore, India, included topics covering new developments in the biopharma industry. This conference report highlights selected presentations on novel therapeutics for the treatment of cancer, including identification of novel benzimidazole, N-subsituted isatin and azetidine derivatives, and an Wnt antagonist. In addition, presentations from several biopharma companies and universities are highlighted, including Proteomics International Pty Ltd, Oxford BioMedica plc, AnaptysBio Inc, SIOGEN Biotech, RV College of Engineering and Indian Institute of Science.
The Bermuda BioOptics Project (BBOP) Years 9-11
NASA Technical Reports Server (NTRS)
Maritorena, S.; Siegel, D. A.; Nelson, Norm B.
2004-01-01
The Bermuda BioOptics Project (BBOP) is a collaborative effort between the Institute for Computational Earth System Science (ICESS) at the University of California at Santa Barbara (UCSB) and the Bermuda Biological Station for Research (BBSR). This research program is designed to characterize light availability and utilization in the Sargasso Sea, and to provide an optical link by which biogeochemical observations may be used to evaluate bio-optical models for pigment concentration, primary production, and sinking particle fluxes from satellite-based ocean color sensors. The BBOP time-series was initiated in 1992, and is carried out in conjunction with the U.S. JGOFS Bermuda Atlantic Time-series Study (BATS) at the Bermuda Biological Station for Research. The BATS program itself has been observing biogeochemical processes (primary productivity, particle flux and elemental cycles) in the mesotrophic waters of the Sargasso Sea since 1988. Closely affiliated with BBOP and BATS is a separate NASA-funded study of the spatial variability of biogeochemical processes in the Sargasso Sea using high-resolution AVHRR and SeaWiFS data collected at Bermuda. The collaboration between BATS and BBOP measurements has resulted in a unique data set that addresses not only the SIMBIOS goals but also the broader issues of important factors controlling the carbon cycle.
Experimental results for characterization of a tapered plastic optical fiber sensor based on SPR
NASA Astrophysics Data System (ADS)
Cennamo, N.; Galatus, R.; Zeni, L.
2015-05-01
The experimental results obtained with two different Plastic Optical Fiber (POF) geometries, tapered and not-tapered, for a sensor based on Surface Plasmon Resonance (SPR) are presented. SPR is used for determining the refractive index variations at the interface between a gold layer and a dielectric medium (aqueous medium). In this work SPR sensors in POF configurations, useful for bio-sensing applications, have been realized for the optimization of the sensitivity and experimentally tested. The results show as the sensitivity increases with the tapered POF configuration, when the refractive index of aqueous medium increases.
Dukic, Maja; Winhold, Marcel; Schwalb, Christian H.; Adams, Jonathan D.; Stavrov, Vladimir; Huth, Michael; Fantner, Georg E.
2016-01-01
The sensitivity and detection speed of cantilever-based mechanical sensors increases drastically through size reduction. The need for such increased performance for high-speed nanocharacterization and bio-sensing, drives their sub-micrometre miniaturization in a variety of research fields. However, existing detection methods of the cantilever motion do not scale down easily, prohibiting further increase in the sensitivity and detection speed. Here we report a nanomechanical sensor readout based on electron co-tunnelling through a nanogranular metal. The sensors can be deposited with lateral dimensions down to tens of nm, allowing the readout of nanoscale cantilevers without constraints on their size, geometry or material. By modifying the inter-granular tunnel-coupling strength, the sensors' conductivity can be tuned by up to four orders of magnitude, to optimize their performance. We show that the nanoscale printed sensors are functional on 500 nm wide cantilevers and that their sensitivity is suited even for demanding applications such as atomic force microscopy. PMID:27666316
Long-wavelength analyte-sensitive luminescent probes and optical (bio)sensors
Staudinger, Christoph; Borisov, Sergey M
2016-01-01
Long-wavelength luminescent probes and sensors become increasingly popular. They offer the advantage of lower levels of autofluorescence in most biological probes. Due to high penetration depth and low scattering of red and NIR light such probes potentially enable in vivo measurements in tissues and some of them have already reached a high level of reliability required for such applications. This review focuses on the recent progress in development and application of long-wavelength analyte-sensitive probes which can operate both reversibly and irreversibly. Photophysical properties, sensing mechanisms, advantages and limitations of individual probes are discussed. PMID:27134748
Xu, Quan; Liu, Yao; Su, Rigu; Cai, Lulu; Li, Bofan; Zhang, Yingyuan; Zhang, Linzhou; Wang, Yajun; Wang, Yan; Li, Neng; Gong, Xiao; Gu, Zhipeng; Chen, Yusheng; Tan, Yanglan; Dong, Chenbo; Sreeprasad, Theruvakkattil Sreenivasan
2016-10-20
Heteroatom doped carbon dots (CDs), with high photoluminescence quantum yield (PLQY), are of keen interest in various applications such as chemical sensors, bio-imaging, electronics, and photovoltaics. Zinc, an important element assisting the electron-transfer process and an essential trace element for cells, is a promising metal dopant for CDs, which could potentially lead to multifunctional CDs. In this contribution, we report a single-step, high efficiency, hydrothermal method to synthesize Zn-doped carbon dots (Zn-CDs) with a superior PLQY. The PLQY and luminescence characteristic of Zn-CDs can be tuned by controlling the precursor ratio, and the surface oxidation in the CDs. Though a few studies have reported metal doped CDs with good PLQY, the as prepared Zn-Cds in the present method exhibited a PLQY up to 32.3%. To the best of our knowledge, there is no report regarding the facile preparation of single metal-doped CDs with a QY more than 30%. Another unique attribute of the Zn-CDs is the high monodispersity and the resultant highly robust excitation-independent luminescence that is stable over a broad range of pH values. Spectroscopic investigations indicated that the superior PLQY and luminescence of Zn-CDs are due to the heteroatom directed, oxidized carbon-based surface passivation. Furthermore, we developed a novel and sensitive biosensor for the detection of hydrogen peroxide and glucose leveraging the robust fluorescence properties of Zn-CDs. Under optimal conditions, Zn-CDs demonstrated high sensitivity and response to hydrogen peroxide and glucose over a wide range of concentrations, with a linear range of 10-80 μM and 5-100 μM, respectively, indicating their great potential as a fluorescent probe for chemical sensing.
Electrochemical MIP-Sensors for Drugs.
Yarman, Aysu; Kurbanoglu, Sevinc; Jetzschmann, Katharina J; Ozkan, Sibel A; Wollenberger, Ulla; Scheller, Frieder
2017-10-05
In order to replace bio-macromolecules by stable synthetic materials in separation techniques and bioanalysis biomimetic receptors and catalysts have been developed: Functional monomers are polymerized together with the target analyte and after template removal cavities are formed in the "molecularly imprinted polymer" (MIP) which resemble the active sites of antibodies and enzymes. Staring almost 80 years ago, around 1,100 papers on MIPs were published in 2016. Electropolymerization allows to deposit MIPs directly on voltammetric electrodes or chips for quartz crystal microbalance (QCM) and surface plasmon resonance (SPR). For the readout of MIPs for drugs amperometry, differential pulse voltammetry (DPV) and impedance spectroscopy (EIS) offer higher sensitivity as compared with QCM or SPR. Application of simple electrochemical devices allows both the reproducible preparation of MIP sensors, but also the sensitive signal generation. Electrochemical MIP-sensors for the whole arsenal of drugs, e.g. the most frequently used analgesics, antibiotics and anticancer drugs have been presented in literature and tested under laboratory conditions. These biomimetic sensors typically have measuring ranges covering the lower nano- up to millimolar concentration range and they are stable under extreme pH and in organic solvents like non-aqueous extracts. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.
Clinical application of bio ceramics
NASA Astrophysics Data System (ADS)
Anu, Sharma; Gayatri, Sharma
2016-05-01
Ceramics are the inorganic crystalline material. These are used in various field such as biomedical, electrical, electronics, aerospace, automotive and optical etc. Bio ceramics are the one of the most active areas of research. Bio ceramics are the ceramics which are biocompatible. The unique properties of bio ceramics make them an attractive option for medical applications and offer some potential advantages over other materials. During the past three decades, a number of major advances have been made in the field of bio ceramics. This review focuses on the use of these materials in variety of clinical scenarios.
Clinical application of bio ceramics
DOE Office of Scientific and Technical Information (OSTI.GOV)
Anu, Sharma, E-mail: issaranu@gmail.com; Gayatri, Sharma, E-mail: sharmagayatri@gmail.com
Ceramics are the inorganic crystalline material. These are used in various field such as biomedical, electrical, electronics, aerospace, automotive and optical etc. Bio ceramics are the one of the most active areas of research. Bio ceramics are the ceramics which are biocompatible. The unique properties of bio ceramics make them an attractive option for medical applications and offer some potential advantages over other materials. During the past three decades, a number of major advances have been made in the field of bio ceramics. This review focuses on the use of these materials in variety of clinical scenarios.
BioNetFit: a fitting tool compatible with BioNetGen, NFsim and distributed computing environments
Thomas, Brandon R.; Chylek, Lily A.; Colvin, Joshua; ...
2015-11-09
Rule-based models are analyzed with specialized simulators, such as those provided by the BioNetGen and NFsim open-source software packages. Here in this paper, we present BioNetFit, a general-purpose fitting tool that is compatible with BioNetGen and NFsim. BioNetFit is designed to take advantage of distributed computing resources. This feature facilitates fitting (i.e. optimization of parameter values for consistency with data) when simulations are computationally expensive.
BioNetFit: a fitting tool compatible with BioNetGen, NFsim and distributed computing environments.
Thomas, Brandon R; Chylek, Lily A; Colvin, Joshua; Sirimulla, Suman; Clayton, Andrew H A; Hlavacek, William S; Posner, Richard G
2016-03-01
Rule-based models are analyzed with specialized simulators, such as those provided by the BioNetGen and NFsim open-source software packages. Here, we present BioNetFit, a general-purpose fitting tool that is compatible with BioNetGen and NFsim. BioNetFit is designed to take advantage of distributed computing resources. This feature facilitates fitting (i.e. optimization of parameter values for consistency with data) when simulations are computationally expensive. BioNetFit can be used on stand-alone Mac, Windows/Cygwin, and Linux platforms and on Linux-based clusters running SLURM, Torque/PBS, or SGE. The BioNetFit source code (Perl) is freely available (http://bionetfit.nau.edu). Supplementary data are available at Bioinformatics online. bionetgen.help@gmail.com. © The Author 2015. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.
Three-dimensional bio-printing.
Gu, Qi; Hao, Jie; Lu, YangJie; Wang, Liu; Wallace, Gordon G; Zhou, Qi
2015-05-01
Three-dimensional (3D) printing technology has been widely used in various manufacturing operations including automotive, defence and space industries. 3D printing has the advantages of personalization, flexibility and high resolution, and is therefore becoming increasingly visible in the high-tech fields. Three-dimensional bio-printing technology also holds promise for future use in medical applications. At present 3D bio-printing is mainly used for simulating and reconstructing some hard tissues or for preparing drug-delivery systems in the medical area. The fabrication of 3D structures with living cells and bioactive moieties spatially distributed throughout will be realisable. Fabrication of complex tissues and organs is still at the exploratory stage. This review summarize the development of 3D bio-printing and its potential in medical applications, as well as discussing the current challenges faced by 3D bio-printing.
Minchew, Candace L.; Didenko, Vladimir V.
2014-01-01
We describe a new type of bio-nanomachine which runs on thermal noise. The machine is solely powered by the random motion of water molecules in its environment and does not ever require re-fuelling. The construct, which is made of DNA and vaccinia virus topoisomerase protein, can detect DNA damage by employing fluorescence. It uses Brownian motion as a cyclic motor to continually separate and bring together two types of fluorescent hairpins participating in FRET. This bio-molecular oscillator is a fast and specific sensor of 5′OH double-strand DNA breaks present in phagocytic phase of apoptosis. The detection takes 30 s in solution and 3 min in cell suspensions. The phagocytic phase is critical for the effective execution of apoptosis as it ensures complete degradation of the dying cells’ DNA, preventing release of pathological, viral and tumor DNA and self-immunization. The construct can be used as a smart FRET probe in studies of cell death and phagocytosis. PMID:25268504
Pemberton, Roy M.; Cox, Timothy; Tuffin, Rachel; Drago, Guido A.; Griffiths, John; Pittson, Robin; Johnson, Graham; Xu, Jinsheng; Sage, Ian C.; Davies, Rhodri; Jackson, Simon K.; Kenna, Gerry; Luxton, Richard; Hart, John P.
2014-01-01
This report describes the design and development of an integrated electrochemical cell culture monitoring system, based on enzyme-biosensors and chemical sensors, for monitoring indicators of mammalian cell metabolic status. MEMS technology was used to fabricate a microwell-format silicon platform including a thermometer, onto which chemical sensors (pH, O2) and screen-printed biosensors (glucose, lactate), were grafted/deposited. Microwells were formed over the fabricated sensors to give 5-well sensor strips which were interfaced with a multipotentiostat via a bespoke connector box interface. The operation of each sensor/biosensor type was examined individually, and examples of operating devices in five microwells in parallel, in either potentiometric (pH sensing) or amperometric (glucose biosensing) mode are shown. The performance characteristics of the sensors/biosensors indicate that the system could readily be applied to cell culture/toxicity studies. PMID:25360580
Dickinson, Paul A; Kesisoglou, Filippos; Flanagan, Talia; Martinez, Marilyn N; Mistry, Hitesh B; Crison, John R; Polli, James E; Cruañes, Maria T; Serajuddin, Abu T M; Müllertz, Anette; Cook, Jack A; Selen, Arzu
2016-11-01
The aim of Biopharmaceutics Risk Assessment Roadmap (BioRAM) and the BioRAM Scoring Grid is to facilitate optimization of clinical performance of drug products. BioRAM strategy relies on therapy-driven drug delivery and follows an integrated systems approach for formulating and addressing critical questions and decision-making (J Pharm Sci. 2014,103(11): 3777-97). In BioRAM, risk is defined as not achieving the intended in vivo drug product performance, and success is assessed by time to decision-making and action. Emphasis on time to decision-making and time to action highlights the value of well-formulated critical questions and well-designed and conducted integrated studies. This commentary describes and illustrates application of the BioRAM Scoring Grid, a companion to the BioRAM strategy, which guides implementation of such an integrated strategy encompassing 12 critical areas and 6 assessment stages. Application of the BioRAM Scoring Grid is illustrated using published literature. Organizational considerations for implementing BioRAM strategy, including the interactions, function, and skillsets of the BioRAM group members, are also reviewed. As a creative and innovative systems approach, we believe that BioRAM is going to have a broad-reaching impact, influencing drug development and leading to unique collaborations influencing how we learn, and leverage and share knowledge. Published by Elsevier Inc.
Garcia-Perez, Manuel; Adams, Thomas T; Goodrum, John W; Das, K C; Geller, Daniel P
2010-08-01
This paper describes the use of Differential Scanning Calorimetry (DSC) to evaluate the impact of varying mix ratios of bio-oil (pyrolysis oil) and bio-diesel on the oxidation stability and on some cold flow properties of resulting blends. The bio-oils employed were produced from the semi-continuous Auger pyrolysis of pine pellets and the batch pyrolysis of pine chips. The bio-diesel studied was obtained from poultry fat. The conditions used to prepare the bio-oil/bio-diesel blends as well as some of the fuel properties of these blends are reported. The experimental results suggest that the addition of bio-oil improves the oxidation stability of the resulting blends and modifies the crystallization behavior of unsaturated compounds. Upon the addition of bio-oil an increase in the oxidation onset temperature, as determined by DSC, was observed. The increase in bio-diesel oxidation stability is likely to be due to the presence of hindered phenols abundant in bio-oils. A relatively small reduction in DSC characteristic temperatures which are associated with cold flow properties was also observed but can likely be explained by a dilution effect. (c) 2010 Elsevier Ltd. All rights reserved.
Bio-threat microparticle simulants
Farquar, George Roy; Leif, Roald N
2012-10-23
A bio-threat simulant that includes a carrier and DNA encapsulated in the carrier. Also a method of making a simulant including the steps of providing a carrier and encapsulating DNA in the carrier to produce the bio-threat simulant.
Bio-threat microparticle simulants
Farquar, George Roy; Leif, Roald
2014-09-16
A bio-threat simulant that includes a carrier and DNA encapsulated in the carrier. Also a method of making a simulant including the steps of providing a carrier and encapsulating DNA in the carrier to produce the bio-threat simulant.
Vibro-Perception of Optical Bio-Inspired Fiber-Skin.
Li, Tao; Zhang, Sheng; Lu, Guo-Wei; Sunami, Yuta
2018-05-12
In this research, based on the principle of optical interferometry, the Mach-Zehnder and Optical Phase-locked Loop (OPLL) vibro-perception systems of bio-inspired fiber-skin are designed to mimic the tactile perception of human skin. The fiber-skin is made of the optical fiber embedded in the silicone elastomer. The optical fiber is an instinctive and alternative sensor for tactile perception with high sensitivity and reliability, also low cost and susceptibility to the magnetic interference. The silicone elastomer serves as a substrate with high flexibility and biocompatibility, and the optical fiber core serves as the vibro-perception sensor to detect physical motions like tapping and sliding. According to the experimental results, the designed optical fiber-skin demonstrates the ability to detect the physical motions like tapping and sliding in both the Mach-Zehnder and OPLL vibro-perception systems. For direct contact condition, the OPLL vibro-perception system shows better performance compared with the Mach-Zehnder vibro-perception system. However, the Mach-Zehnder vibro-perception system is preferable to the OPLL system in the indirect contact experiment. In summary, the fiber-skin is validated to have light touch character and excellent repeatability, which is highly-suitable for skin-mimic sensing.
Hokuto, Toshiki; Yasukawa, Tomoyuki; Kunikata, Ryota; Suda, Atsushi; Inoue, Kumi Y; Ino, Kosuke; Matsue, Tomokazu; Mizutani, Fumio
2016-06-01
Electrochemical imaging is an excellent technique to characterize an activity of biomaterials, such as enzymes and cells. Large scale integration-based amperometric sensor (Bio-LSI) has been developed for the simultaneous and continuous detection of the concentration distribution of redox species generated by reactions of biomolecules. In this study, the Bio-LSI system was demonstrated to be applicable for simultaneous detection of different anaytes in multiple specimens. The multiple specimens containing human immunoglobulin G (hIgG) and mouse IgG (mIgG) were introduced into each channel of the upper substrate across the antibody lines for hIgG and mIgG on the lower substrate. Hydrogen peroxide generated by the enzyme reaction of glucose oxidase captured at intersections was simultaneously detected by 400 microelectrodes of Bio-LSI chip. The oxidation current increased with increasing the concentrations of hIgG, which can be detected in the range of 0.01-1.0 µg mL(-1) . Simultaneous detection of hIgG and mIgG in multiple specimens was achieved by using line pattern of both antibodies. Therefore, the presence of different target molecules in the multiple samples would be quantitatively and simultaneously visualized as a current image by the Bio-LSI system. Copyright © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Negated bio-events: analysis and identification
2013-01-01
Background Negation occurs frequently in scientific literature, especially in biomedical literature. It has previously been reported that around 13% of sentences found in biomedical research articles contain negation. Historically, the main motivation for identifying negated events has been to ensure their exclusion from lists of extracted interactions. However, recently, there has been a growing interest in negative results, which has resulted in negation detection being identified as a key challenge in biomedical relation extraction. In this article, we focus on the problem of identifying negated bio-events, given gold standard event annotations. Results We have conducted a detailed analysis of three open access bio-event corpora containing negation information (i.e., GENIA Event, BioInfer and BioNLP’09 ST), and have identified the main types of negated bio-events. We have analysed the key aspects of a machine learning solution to the problem of detecting negated events, including selection of negation cues, feature engineering and the choice of learning algorithm. Combining the best solutions for each aspect of the problem, we propose a novel framework for the identification of negated bio-events. We have evaluated our system on each of the three open access corpora mentioned above. The performance of the system significantly surpasses the best results previously reported on the BioNLP’09 ST corpus, and achieves even better results on the GENIA Event and BioInfer corpora, both of which contain more varied and complex events. Conclusions Recently, in the field of biomedical text mining, the development and enhancement of event-based systems has received significant interest. The ability to identify negated events is a key performance element for these systems. We have conducted the first detailed study on the analysis and identification of negated bio-events. Our proposed framework can be integrated with state-of-the-art event extraction systems. The
Bio-Liquid Morphological Analysis
Shatokhina, S.N.; Shabalin, V.N.; Buzoverya, M.E.; Punin, V.T.
2004-01-01
Information is presented on the new scientific line in medicine and biology: bio-liquid morphology. The interdisciplinary character of the given research area is emphasized. The problems and prospects of bio-liquid morphological analysis development both in applied and fundamental aspects are discussed. PMID:15349509
Two 1,8- Naphthalimides as Proton-Receptor Fluorescent Sensors for Detecting PH
NASA Astrophysics Data System (ADS)
Wu, H.-L.; Peng, H.-P.; Wang, F.; Zhang, H.; Chen, C.-G.; Zhang, J.-W.; Yang, Z.-H.
2017-01-01
Two proton-receptor sensors for detecting pH change based on 1,8-naphthalimide, N-allyl-4-(4'-N,N-dioctylpropionamide-acetamido-piperazinyl)-1,8-naphthalimide ( 1), and N-(N,N-dioctylpropionamide-acetamido)-4-allyl-1-piperazinyl-1,8-naphthalimide ( 2), were designed, synthesized, and characterized. Photophysical characteristics of the sensors were investigated in different organic solvents and Britton-Robinson buffer/EtOH (1:1, v/v) solution. Sensor 2 displayed a good sensor activity towards protons within the pH range from 3.29 to 6.59, while sensor 1 demonstrated sensitivity to lower pH values from 2.21 to 4.35. The selectivity of the pH sensors toward protons in commonly used buffer solutions and in the presence of metal cations (Na+, K+, Ca2+, Mg2+, Al3+, Pb2+, Fe3+, Ni2+, Zn2+, Cu2+, Hg2+, Ag+, Co2+, Cr3+, Mn2+, and Cd2+) was studied by monitoring the changes in their fluorescence intensity. The results obtained indicate that the synthesized derivatives hold potential for monitoring pH variations between 2.21 and 6.59 in strong acid environments and bio-samples.
Biostability of an implantable glucose sensor chip
NASA Astrophysics Data System (ADS)
Fröhlich, M.; Birkholz, M.; Ehwald, K. E.; Kulse, P.; Fursenko, O.; Katzer, J.
2012-12-01
Surface materials of an implantable microelectronic chip intended for medical applications were evaluated with respect to their long-term stability in bio-environments. The sensor chip shall apply in a glucose monitor by operating as a microviscosimeter according to the principle of affinity viscosimetry. A monolithic integration of a microelectromechanical system (MEMS) into the sensor chip was successfully performed in a combined 0.25 μm CMOS/BiCMOS technology. In order to study material durability and biostability of the surfaces, sensor chips were exposed to various in vitro and in vivo tests. Corrosional damage of SiON, SiO2 and TiN surfaces was investigated by optical microscopy, ellipsometry and AFM. The results served for optimizing the Back-end-of-Line (BEoL) stack, from which the MEMS was prepared. Corrosion of metal lines could significantly be reduced by improving the topmost passivation layer. The experiments revealed no visible damage of the actuator or other functionally important MEMS elements. Sensor chips were also exposed to human body fluid for three month by implantation into the abdomen of a volunteer. Only small effects were observed for layer thickness and Ra roughness after explantation. In particular, TiN as used for the actuator beam showed no degradation by biocorrosion. The highest degradation rate of about 50 nm per month was revealed for the SiON passivation layer. These results suggest that the sensor chip may safely operate in subcutaneous tissue for a period of several months.
Bio-Oil Analysis Laboratory Procedures | Bioenergy | NREL
Bio-Oil Analysis Laboratory Procedures Bio-Oil Analysis Laboratory Procedures NREL develops standard procedures have been validated and allow for reliable bio-oil analysis. Procedures Determination different hydroxyl groups (-OH) in pyrolysis bio-oil: aliphatic-OH, phenolic-OH, and carboxylic-OH. Download
A Soft Sensor for Bioprocess Control Based on Sequential Filtering of Metabolic Heat Signals
Paulsson, Dan; Gustavsson, Robert; Mandenius, Carl-Fredrik
2014-01-01
Soft sensors are the combination of robust on-line sensor signals with mathematical models for deriving additional process information. Here, we apply this principle to a microbial recombinant protein production process in a bioreactor by exploiting bio-calorimetric methodology. Temperature sensor signals from the cooling system of the bioreactor were used for estimating the metabolic heat of the microbial culture and from that the specific growth rate and active biomass concentration were derived. By applying sequential digital signal filtering, the soft sensor was made more robust for industrial practice with cultures generating low metabolic heat in environments with high noise level. The estimated specific growth rate signal obtained from the three stage sequential filter allowed controlled feeding of substrate during the fed-batch phase of the production process. The biomass and growth rate estimates from the soft sensor were also compared with an alternative sensor probe and a capacitance on-line sensor, for the same variables. The comparison showed similar or better sensitivity and lower variability for the metabolic heat soft sensor suggesting that using permanent temperature sensors of a bioreactor is a realistic and inexpensive alternative for monitoring and control. However, both alternatives are easy to implement in a soft sensor, alone or in parallel. PMID:25264951
A soft sensor for bioprocess control based on sequential filtering of metabolic heat signals.
Paulsson, Dan; Gustavsson, Robert; Mandenius, Carl-Fredrik
2014-09-26
Soft sensors are the combination of robust on-line sensor signals with mathematical models for deriving additional process information. Here, we apply this principle to a microbial recombinant protein production process in a bioreactor by exploiting bio-calorimetric methodology. Temperature sensor signals from the cooling system of the bioreactor were used for estimating the metabolic heat of the microbial culture and from that the specific growth rate and active biomass concentration were derived. By applying sequential digital signal filtering, the soft sensor was made more robust for industrial practice with cultures generating low metabolic heat in environments with high noise level. The estimated specific growth rate signal obtained from the three stage sequential filter allowed controlled feeding of substrate during the fed-batch phase of the production process. The biomass and growth rate estimates from the soft sensor were also compared with an alternative sensor probe and a capacitance on-line sensor, for the same variables. The comparison showed similar or better sensitivity and lower variability for the metabolic heat soft sensor suggesting that using permanent temperature sensors of a bioreactor is a realistic and inexpensive alternative for monitoring and control. However, both alternatives are easy to implement in a soft sensor, alone or in parallel.
Comparison of different photoresist buffer layers in SPR sensors based on D-shaped POF and gold film
NASA Astrophysics Data System (ADS)
Cennamo, Nunzio; Pesavento, Maria; De Maria, Letizia; Galatus, Ramona; Mattiello, Francesco; Zeni, Luigi
2017-04-01
A comparative analysis of two optical fiber sensing platforms is presented. The sensors are based on surface plasmon resonance (SPR) in a D-shaped plastic optical fiber (POF) with a photoresist buffer layer between the exposed POF core and the thin gold film. We show how the sensor's performances change when the photoresist layer changes. The photoresist layers proposed in this analysis are SU-8 3005 and S1813. The experimental results are congruent with the numerical studies and it is instrumental for chemical and bio-chemical applications. Usually, the photoresist layer is required in order to increase the performance of the SPR-POF sensor.
BioASF: a framework for automatically generating executable pathway models specified in BioPAX.
Haydarlou, Reza; Jacobsen, Annika; Bonzanni, Nicola; Feenstra, K Anton; Abeln, Sanne; Heringa, Jaap
2016-06-15
Biological pathways play a key role in most cellular functions. To better understand these functions, diverse computational and cell biology researchers use biological pathway data for various analysis and modeling purposes. For specifying these biological pathways, a community of researchers has defined BioPAX and provided various tools for creating, validating and visualizing BioPAX models. However, a generic software framework for simulating BioPAX models is missing. Here, we attempt to fill this gap by introducing a generic simulation framework for BioPAX. The framework explicitly separates the execution model from the model structure as provided by BioPAX, with the advantage that the modelling process becomes more reproducible and intrinsically more modular; this ensures natural biological constraints are satisfied upon execution. The framework is based on the principles of discrete event systems and multi-agent systems, and is capable of automatically generating a hierarchical multi-agent system for a given BioPAX model. To demonstrate the applicability of the framework, we simulated two types of biological network models: a gene regulatory network modeling the haematopoietic stem cell regulators and a signal transduction network modeling the Wnt/β-catenin signaling pathway. We observed that the results of the simulations performed using our framework were entirely consistent with the simulation results reported by the researchers who developed the original models in a proprietary language. The framework, implemented in Java, is open source and its source code, documentation and tutorial are available at http://www.ibi.vu.nl/programs/BioASF CONTACT: j.heringa@vu.nl. © The Author 2016. Published by Oxford University Press.
A case study on bio-optical and radiometric quantities in northwest European shelf seas
NASA Astrophysics Data System (ADS)
Garaba, Shungu; Zielinski, Oliver
2013-04-01
Colour of seawater has become an integral tool in understanding surface marine ecosystems and processes. Additionally, operational oceanographic observatories are becoming more prominent these days while at the same time hyperspectral radiometric sensors are becoming increasingly affordable. This has driven a wide spread use of these hyperspectral sensors to measure reflectance above the water surface from stationary and mobile platforms alike. As enormous amounts of data are produced and favourably processed in real-time, effective quality control procedures become more than just supporting tools, but a crucial prerequisite for trustworthy and manageable information. Here, we use bio-geophysical and hyperspectral radiometric measurements from German Bight (GB), North Sea (NS), Inner Seas (ISS), Irish Sea (IS) and Celtic Sea (CS) to identify and establish relationships between colour producing agents (CPAs) and perceived colour of seawater. In order to obtain valid optical measurements, meteorological and sunglint contamination were mitigated using state-of-the-art quality control protocols. The remote sensing reflectance measured is transformed into discrete Forel-Ule numerical indices (FUI), 1 (indigo-blue, oligotrophic) to 21 (cola brown, hyper-eutrophic). We present a novel approach of estimating which of the three main CPAs of seawater control perceived colour of seawater. Our bio-optical models for estimating FUI for measured CPAs; chlorophyll (Chl-a), coloured dissolved organic material (CDOM) and suspended particulate material (SPM) had correlation coefficients, R² (GB = 0.98, NS = 0.23, ISS=0.99, IS=0.63, CS = 0.16). It was also observed that salinity can be estimated from coloured dissolved organic matter with good accuracy, R² (GB = 0.94, NS = 0.44, ISS=0.90, IS=0.85, CS = 0.51). We show that ocean colour products i.e. reflectance and perceived colour of seawater can be used to infer, with good accuracy, environmental parameters e.g. Chl-a, CDOM, SPM
NASA Astrophysics Data System (ADS)
Jolliff, Jason K.; Kindle, John C.; Penta, Bradley; Helber, Robert; Lee, Zhongping; Shulman, Igor; Arnone, Robert; Rowley, Clark D.
2008-03-01
Three years of Sea-viewing Wide Field-of-view Sensor (SeaWiFS) ocean color data were combined with three-dimensional thermal fields generated by the U.S. Navy's Modular Ocean Data Assimilation System (MODAS) in order to examine the interdependencies between bio-optical fields and their relationship to seasonal and mesoscale changes in upper ocean thermal structure. The combined data set suggests that the oceanic boundary layer within the Gulf of Mexico may be broadly defined by two seasonally occurring bio-thermal periods. A winter mixing period, characterized by net heat losses to the atmosphere, deepening of the isothermal layer depth, and annual maxima of satellite-estimated colored detrital matter (CDM) absorption coefficients and surface pigment concentration, was followed by a thermally stratified period characterized by net surface ocean heating, reduced isothermal layer depths, and annual minima in surface bio-optical fields. Variability in the interdependencies of ocean color products was used to diagnose an attendant shift in the size-structure of surface phytoplankton communities as well as identify CDM as the constituent responsible for the majority of blue-light absorption in Gulf of Mexico surface waters. The mesoscale circulation, as resolved by MODAS thermal fields into cold and warm-core eddies, appears to significantly modulate the seasonal bio-optical cycle of CDM absorption and surface pigment concentration. An empirical model was developed to describe CDM absorption as a function of upper ocean thermal energy. The model accounted for nearly half the variance in the satellite-estimate of this bio-optical variable. Large mismatches between the model and satellite data implied episodes of shelf water export to the deep Gulf of Mexico.
Islamaj Doğan, Rezarta; Comeau, Donald C; Yeganova, Lana; Wilbur, W John
2014-01-01
BioC is a recently created XML format to share text data and annotations, and an accompanying input/output library to promote interoperability of data and tools for natural language processing of biomedical text. This article reports the use of BioC to address a common challenge in processing biomedical text information-that of frequent entity name abbreviation. We selected three different abbreviation definition identification modules, and used the publicly available BioC code to convert these independent modules into BioC-compatible components that interact seamlessly with BioC-formatted data, and other BioC-compatible modules. In addition, we consider four manually annotated corpora of abbreviations in biomedical text: the Ab3P corpus of 1250 PubMed abstracts, the BIOADI corpus of 1201 PubMed abstracts, the old MEDSTRACT corpus of 199 PubMed(®) citations and the Schwartz and Hearst corpus of 1000 PubMed abstracts. Annotations in these corpora have been re-evaluated by four annotators and their consistency and quality levels have been improved. We converted them to BioC-format and described the representation of the annotations. These corpora are used to measure the three abbreviation-finding algorithms and the results are given. The BioC-compatible modules, when compared with their original form, have no difference in their efficiency, running time or any other comparable aspects. They can be conveniently used as a common pre-processing step for larger multi-layered text-mining endeavors. Database URL: Code and data are available for download at the BioC site: http://bioc.sourceforge.net. Published by Oxford University Press 2014. This work is written by US Government employees and is in the public domain in the US.
Bio-inspired patterned networks (BIPS) for development of wearable/disposable biosensors
NASA Astrophysics Data System (ADS)
McLamore, E. S.; Convertino, M.; Hondred, John; Das, Suprem; Claussen, J. C.; Vanegas, D. C.; Gomes, C.
2016-05-01
Here we demonstrate a novel approach for fabricating point of care (POC) wearable electrochemical biosensors based on 3D patterning of bionanocomposite networks. To create Bio-Inspired Patterned network (BIPS) electrodes, we first generate fractal network in silico models that optimize transport of network fluxes according to an energy function. Network patterns are then inkjet printed onto flexible substrate using conductive graphene ink. We then deposit fractal nanometal structures onto the graphene to create a 3D nanocomposite network. Finally, we biofunctionalize the surface with biorecognition agents using covalent bonding. In this paper, BIPS are used to develop high efficiency, low cost biosensors for measuring glucose as a proof of concept. Our results on the fundamental performance of BIPS sensors show that the biomimetic nanostructures significantly enhance biosensor sensitivity, accuracy, response time, limit of detection, and hysteresis compared to conventional POC non fractal electrodes (serpentine, interdigitated, and screen printed electrodes). BIPs, in particular Apollonian patterned BIPS, represent a new generation of POC biosensors based on nanoscale and microscale fractal networks that significantly improve electrical connectivity, leading to enhanced sensor performance.
Safety Aspects of Bio-Based Nanomaterials.
Catalán, Julia; Norppa, Hannu
2017-12-01
Moving towards a bio-based and circular economy implies a major focus on the responsible and sustainable utilization of bio-resources. The emergence of nanotechnology has opened multiple possibilities, not only in the existing industrial sectors, but also for completely novel applications of nanoscale bio-materials, the commercial exploitation of which has only begun during the last few years. Bio-based materials are often assumed not to be toxic. However, this pre-assumption is not necessarily true. Here, we provide a short overview on health and environmental aspects associated with bio-based nanomaterials, and on the relevant regulatory requirements. We also discuss testing strategies that may be used for screening purposes at pre-commercial stages. Although the tests presently used to reveal hazards are still evolving, regarding modifi-cations required for nanomaterials, their application is needed before the upscaling or commercialization of bio-based nanomaterials, to ensure the market potential of the nanomaterials is not delayed by uncertainties about safety issues.
[Current status of bio-based materials industry in China].
Diao, Xiaoqian; Weng, Yunxuan; Huang, Zhigang; Yang, Nan; Wang, Xiyuan; Zhang, Min; Jin, Yujuan
2016-06-25
In recent years, bio-based materials are becoming a new dominant industry leading the scientific and technological innovation, and economic development of the world. We reviewed the new development of bio-based materials industry in China, analyzed the entire market of bio-based materials products comprehensively, and also stated the industry status of bio-based chemicals, such as lactic acid, 1,3-propanediol, and succinic acid; biodegradable bio-based polymers, such as co-polyester of diacid and diol, polylactic acid, carbon dioxide based copolymer, polyhydroxyalknoates, polycaprolactone, and thermoplastic bio-based plastics; non-biodegradable bio-based polymers, such as bio-based polyamide, polytrimethylene terephthalate, bio-based polyurethane, and bio-based fibers.
Iron-Based Nanomaterials/Graphene Composites for Advanced Electrochemical Sensors
Movlaee, Kaveh; Ganjali, Mohmmad Reza; Norouzi, Parviz
2017-01-01
Iron oxide nanostructures (IONs) in combination with graphene or its derivatives—e.g., graphene oxide and reduced graphene oxide—hold great promise toward engineering of efficient nanocomposites for enhancing the performance of advanced devices in many applicative fields. Due to the peculiar electrical and electrocatalytic properties displayed by composite structures in nanoscale dimensions, increasing efforts have been directed in recent years toward tailoring the properties of IONs-graphene based nanocomposites for developing more efficient electrochemical sensors. In the present feature paper, we first reviewed the various routes for synthesizing IONs-graphene nanostructures, highlighting advantages, disadvantages and the key synthesis parameters for each method. Then, a comprehensive discussion is presented in the case of application of IONs-graphene based composites in electrochemical sensors for the determination of various kinds of (bio)chemical substances. PMID:29168771
Optical Properties of Plasmonic Nanostructures for Bio-Imaging and Bio-Sensing Applications
NASA Astrophysics Data System (ADS)
Kravets, Vira V.
Kravets, Vira V. (Ph.D., Physics). Optical properties of plasmonic nanostructures for bio-imaging and bio-sensing applications. Dissertation directed by Associate Professor Anatoliy Pinchuk. ABSTRACT. This dissertation explores the physics of free electron excitations in gold nanoparticle chains, silver nanoparticle colloids, and thin gold films. Electron excitations in nanostructures (surface plasmons, SP) are responsible for unique optical properties, which are applied in bio-sensing and bio-imaging applications. For gold nanoparticle chains, the effect of SP on resonance light absorption was studied experimentally and theoretically. Mainly, how the spectral position of the absorption peak depends on inter-particle distances. This dependence is used in “molecular rulers”, providing spatial resolution below the Rayleigh limit. The underlying theory is based on particle interaction via scattered dipole fields. Often in literature only the near-field component of the scattered field is considered. Here, I show that middle and far fields should not be neglected for calculation of extinction by particle chains. In silver nanoparticles, SP excitations produce two independent effects: (a) the intrinsic fluorescence of the particles, and (b) the enhancement of a molecule’s fluorescence by a particle’s surface. The mechanism of (a) is deduced by studying how fluorescence depends on particle size. For (b), I show that fluorescence of a dye molecule on the surface of a nanoparticle is enhanced, when compared to that of the free-standing dye. I demonstrate that the dye’s fluorescent quantum yield is dependent on the particle’s size, making labeled silver nanoparticles attractive candidates as bio-imaging agents. Labeled nanoparticles are applied to cell imaging, and their bio-compatibility with two cell lines is evaluated here. Finally, in gold films under attenuated total internal reflection (ATR) conditions, the SP create a propagating wave (SP-polariton, SPP
Matsuba, Sota; Kato, Ryo; Okumura, Koichi; Sawada, Kazuaki; Hattori, Toshiaki
2018-01-01
In biochemistry, Ca 2+ and K + play essential roles to control signal transduction. Much interest has been focused on ion-imaging, which facilitates understanding of their ion flux dynamics. In this paper, we report a calcium and potassium multi-ion image sensor and its application to living cells (PC12). The multi-ion sensor had two selective plasticized poly(vinyl chloride) membranes containing ionophores. Each region on the sensor responded to only the corresponding ion. The multi-ion sensor has many advantages including not only label-free and real-time measurement but also simultaneous detection of Ca 2+ and K + . Cultured PC12 cells treated with nerve growth factor were prepared, and a practical observation for the cells was conducted with the sensor. After the PC12 cells were stimulated by acetylcholine, only the extracellular Ca 2+ concentration increased while there was no increase in the extracellular K + concentration. Through the practical observation, we demonstrated that the sensor was helpful for analyzing the cell events with changing Ca 2+ and/or K + concentration.
Oshima, Masamitsu; Inoue, Kaoru; Nakajima, Kei; Tachikawa, Tetsuhiko; Yamazaki, Hiromichi; Isobe, Tomohide; Sugawara, Ayaka; Ogawa, Miho; Tanaka, Chie; Saito, Masahiro; Kasugai, Shohei; Takano-Yamamoto, Teruko; Inoue, Takashi; Tezuka, Katsunari; Kuboki, Takuo; Yamaguchi, Akira; Tsuji, Takashi
2014-01-01
Bio-hybrid artificial organs are an attractive concept to restore organ function through precise biological cooperation with surrounding tissues in vivo. However, in bio-hybrid artificial organs, an artificial organ with fibrous connective tissues, including muscles, tendons and ligaments, has not been developed. Here, we have enveloped with embryonic dental follicle tissue around a HA-coated dental implant, and transplanted into the lower first molar region of a murine tooth-loss model. We successfully developed a novel fibrous connected tooth implant using a HA-coated dental implant and dental follicle stem cells as a bio-hybrid organ. This bio-hybrid implant restored physiological functions, including bone remodelling, regeneration of severe bone-defect and responsiveness to noxious stimuli, through regeneration with periodontal tissues, such as periodontal ligament and cementum. Thus, this study represents the potential for a next-generation bio-hybrid implant for tooth loss as a future bio-hybrid artificial organ replacement therapy. PMID:25116435
Bio-objects and the media: the role of communication in bio-objectification processes.
Maeseele, Pieter; Allgaier, Joachim; Martinelli, Lucia
2013-06-01
The representation of biological innovations in and through communication and media practices is vital for understanding the nature of "bio-objects" and the process we call "bio-objectification." This paper discusses two ideal-typical analytical approaches based on different underlying communication models, ie, the traditional (science- and media-centered) and media sociological (a multi-layered process involving various social actors in defining the meanings of scientific and technological developments) approach. In this analysis, the latter is not only found to be the most promising approach for understanding the circulation, (re)production, and (re)configuration of meanings of bio-objects, but also to interpret the relationship between media and science. On the basis of a few selected examples, this paper highlights how media function as a primary arena for the (re)production and (re)configuration of scientific and biomedical information with regards to bio-objects in the public sphere in general, and toward decision-makers, interest groups, and the public in specific.
Bio-objects and the media: the role of communication in bio-objectification processes
Maeseele, Pieter; Allgaier, Joachim; Martinelli, Lucia
2013-01-01
The representation of biological innovations in and through communication and media practices is vital for understanding the nature of “bio-objects” and the process we call “bio-objectification.” This paper discusses two ideal-typical analytical approaches based on different underlying communication models, ie, the traditional (science- and media-centered) and media sociological (a multi-layered process involving various social actors in defining the meanings of scientific and technological developments) approach. In this analysis, the latter is not only found to be the most promising approach for understanding the circulation, (re)production, and (re)configuration of meanings of bio-objects, but also to interpret the relationship between media and science. On the basis of a few selected examples, this paper highlights how media function as a primary arena for the (re)production and (re)configuration of scientific and biomedical information with regards to bio-objects in the public sphere in general, and toward decision-makers, interest groups, and the public in specific. PMID:23771763
Aboulkas, A; Hammani, H; El Achaby, M; Bilal, E; Barakat, A; El Harfi, K
2017-11-01
The aim of the present work is to develop processes for the production of bio-oil and bio-char from algae waste using the pyrolysis at controlled conditions. The pyrolysis was carried out at different temperatures 400-600°C and different heating rates 5-50°C/min. The algal waste, bio-oil and bio-char were successfully characterized using Elemental analysis, Chemical composition, TGA, FTIR, 1 H NMR, GC-MS and SEM. At a temperature of 500°C and a heating rate of 10°C/min, the maximum yield of bio-oil and bio-char was found to be 24.10 and 44.01wt%, respectively, which was found to be strongly influenced by the temperature variation, and weakly affected by the heating rate variation. Results show that the bio-oil cannot be used as bio-fuel, but can be used as a source of value-added chemicals. On the other hand, the bio-char is a promising candidate for solid fuel applications and for the production of carbon materials. Copyright © 2017 Elsevier Ltd. All rights reserved.
The Bermuda BioOptics Project (BBOP) Years 9-11
NASA Technical Reports Server (NTRS)
Nelson, Norm
2003-01-01
The Bermuda BioOptics Project (BBOP) is a collaborative effort between the Institute for Computational Earth System Science (ICESS) at the University of California at Santa Barbara (UCSB) and the Bermuda Biological Station for Research (BBSR). This research program is designed to characterize light availability and utilization in the Sargasso Sea, and to provide an optical link by which biogeochemical observations may be used to evaluate bio-optical models for pigment concentration, primary production, and sinking particle fluxes from satellite-based ocean color sensors. The BBOP time-series was initiated in 1992, and is carried out in conjunction with the U.S. JGOFS Bermuda Atlantic Time-series Study (BATS) at the Bermuda Biological Station for Research. The BATS program itself has been observing biogeochemical processes (primary productivity, particle flux and elemental cycles) in the mesotrophic waters of the Sargasso Sea since 1988. Closely affiliated with BBOP and BATS is a separate NASA-funded study of the spatial variability of biogeochemical processes in the Sargasso Sea using high-resolution AVHRR and SeaWiFS data collected at Bermuda. The collaboration between BATS and BBOP measurements has resulted in a unique data set that addresses not only the SIMBIOS goals but also the broader issues of important factors controlling the carbon cycle. This final report addresses specific research activities, research results, and lists of presentations and papers submitted for publication.
ERIC Educational Resources Information Center
Gunersel, Adalet Baris; Fleming, Steven
2014-01-01
Research shows that computer animations are especially helpful in fields such as chemistry and in this mixed-methods study, we investigate the educational effectiveness of Bio-Organic Reaction Animations (BioORA), a 3-D software, in four undergraduate biochemistry classes at different universities. Statistically significant findings indicate that…
Plasmonic nano-sensor based on metal-dielectric-metal waveguide with the octagonal cavity ring
NASA Astrophysics Data System (ADS)
Ghorbani, Saeed; Dashti, Mohammad Ali; Jabbari, Masoud
2018-06-01
In this paper, a refractive index plasmonic sensor including a waveguide of metal–insulator–metal with side coupled octagonal cavity ring has been suggested. The sensory and transmission feature of the structure has been analyzed numerically using Finite Element Method numerical solution. The effect of coupling distance and changing the width of metal–insulator–metal waveguide and refractive index of the dielectric located inside octagonal cavity—which are the effective factors in determining the sensory feature—have been examined so completely that the results of the numerical simulation show a linear relation between the resonance wavelength and refractive index of the liquid/gas dielectric material inside the octagonal cavity ring. High sensitivity of the sensor in the resonance wavelength, simplicity and a compact geometry are the advantages of the refractive plasmonic sensor advised which make that possible to use it for designing high performance nano-sensor and bio-sensing devices.
Leng, Lijian; Yuan, Xingzhong; Shao, Jianguang; Huang, Huajun; Wang, Hou; Li, Hui; Chen, Xiaohong; Zeng, Guangming
2016-01-01
Demetalization of sewage sludge (SS) by sequential extraction before liquefaction was implemented to produce cleaner bio-char and bio-oil. Demetalization steps 1 and 2 did not cause much organic matter loss on SS, and thus the bio-oil and bio-char yields and the compositions of bio-oils were also not affected significantly. However, the demetalization procedures resulted in the production of cleaner bio-chars and bio-oils. The total concentrations and the acid soluble/exchangeable fraction (F1 fraction, the most toxic heavy metal fraction) of heavy metals (Cu, Cr, Pb, Zn, and Cd) in these products were significantly reduced and the environmental risks of these products were also relived considerably compared with those produced from raw SS, respectively. Additionally, these bio-oils had less heavy fractions. Demetalization processes with removal of F1 and F2 fractions of heavy metals would benefit the production of cleaner bio-char and bio-oil by liquefaction of heavy metal abundant biomass like SS. Copyright © 2015 Elsevier Ltd. All rights reserved.
Bio-functionalization of biomedical metals.
Xiao, M; Chen, Y M; Biao, M N; Zhang, X D; Yang, B C
2017-01-01
Bio-functionalization means to endow biomaterials with bio-functions so as to make the materials or devices more suitable for biomedical applications. Traditionally, because of the excellent mechanical properties, the biomedical metals have been widely used in clinic. However, the utilized functions are basically supporting or fixation especially for the implantable devices. Nowadays, some new functions, including bioactivity, anti-tumor, anti-microbial, and so on, are introduced to biomedical metals. To realize those bio-functions on the metallic biomedical materials, surface modification is the most commonly used method. Surface modification, including physical and chemical methods, is an effective way to alter the surface morphology and composition of biomaterials. It can endow the biomedical metals with new surface properties while still retain the good mechanical properties of the bulk material. Having analyzed the ways of realizing the bio-functionalization, this article briefly summarized the bio-functionalization concepts of six hot spots in this field. They are bioactivity, bony tissue inducing, anti-microbial, anti-tumor, anticoagulation, and drug loading functions. Copyright © 2016. Published by Elsevier B.V.
Sarkar, Omprakash; Agarwal, Manu; Naresh Kumar, A; Venkata Mohan, S
2015-02-01
Algal biomass grown hetrotrophically in domestic wastewater was evaluated as pyrolytic feedstock for harnessing biogas, bio-oil and bio-char. Freshly harvested microalgae (MA) and lipid extracted microalgae (LEMA) were pyrolysed in packed bed reactor in the presence and absence of sand as additive. MA (without sand additive) depicted higher biogas (420 ml/g; 800 °C; 3 h) and bio-oil (0.70 ml/g; 500 °C; 3 h). Sand addition enhanced biogas production (210 ml/g; 600 °C; 2 h) in LEMA operation. The composition of bio-gas and bio-oil was found to depend on the nature of feedstock as well as the process conditions viz., pyrolytic-temperature, retention time and presence of additive. Sand additive improved the H2 composition while pyrolytic temperature increment caused a decline in CO2 fraction. Bio-char productivity increased with increasing temperature specifically with LEMA. Integration of thermo-chemical process with microalgae cultivation showed to yield multiple resources and accounts for environmental sustainability in the bio-refinery framework. Copyright © 2014 Elsevier Ltd. All rights reserved.
Plasmonic Waveguide Coupled Ring Cavity for a Non-Resonant Type Refractive Index Sensor.
Kwon, Soon-Hong
2017-11-03
Sensitive refractive index sensors with small footprints have been studied to allow the integration of a large number of sensors into a tiny chip for bio/chemical applications. In particular, resonant-type index sensors based on various micro/nanocavities, which use a resonant wavelength dependence on the refractive index of the analyte, have been developed. However, the spectral linewidth of the resonance, which becomes the resolution limit, is considerably large in plasmonic cavities due to the large absorption loss of metals. Therefore, there is demand for a new type of plasmonic refractive index sensor that is not limited by the linewidth of the cavity. We propose a new type of plasmonic index sensors consisting of a channel waveguide and a ring cavity. Two emissions from the ring cavity in both directions of the waveguide couple with a reflection phase difference depending on the length of a closed right arm with a reflecting boundary. Therefore, the output power dramatically and sensitively changes as a function of the refractive index of the analyte filling the waveguide.
Plasmonic Waveguide Coupled Ring Cavity for a Non-Resonant Type Refractive Index Sensor
Kwon, Soon-Hong
2017-01-01
Sensitive refractive index sensors with small footprints have been studied to allow the integration of a large number of sensors into a tiny chip for bio/chemical applications. In particular, resonant-type index sensors based on various micro/nanocavities, which use a resonant wavelength dependence on the refractive index of the analyte, have been developed. However, the spectral linewidth of the resonance, which becomes the resolution limit, is considerably large in plasmonic cavities due to the large absorption loss of metals. Therefore, there is demand for a new type of plasmonic refractive index sensor that is not limited by the linewidth of the cavity. We propose a new type of plasmonic index sensors consisting of a channel waveguide and a ring cavity. Two emissions from the ring cavity in both directions of the waveguide couple with a reflection phase difference depending on the length of a closed right arm with a reflecting boundary. Therefore, the output power dramatically and sensitively changes as a function of the refractive index of the analyte filling the waveguide. PMID:29099740
DNA decorated carbon nanotube sensors on CMOS circuitry for environmental monitoring
NASA Astrophysics Data System (ADS)
Liu, Yu; Chen, Chia-Ling; Agarwal, V.; Li, Xinghui; Sonkusale, S.; Dokmeci, Mehmet R.; Wang, Ming L.
2010-04-01
Single-walled carbon nanotubes (SWNTs) with their large surface area, high aspect ratio are one of the novel materials which have numerous attractive features amenable for high sensitivity sensors. Several nanotube based sensors including, gas, chemical and biosensors have been demonstrated. Moreover, most of these sensors require off chip components to detect the variations in the signals making them complicated and hard to commercialize. Here we present a novel complementary metal oxide semiconductor (CMOS) integrated carbon nanotube sensors for portable high sensitivity chemical sensing applications. Multiple zincation steps have been developed to ascertain proper electrical connectivity between the carbon nanotubes and the foundry made CMOS circuitry. The SWNTs have been integrated onto (CMOS) circuitry as the feedback resistor of a Miller compensated operational amplifier utilizing low temperature Dielectrophoretic (DEP) assembly process which has been tailored to be compatible with the post-CMOS integration at the die level. Building nanotube sensors directly on commercial CMOS circuitry allows single chip solutions eliminating the need for long parasitic lines and numerous wire bonds. The carbon nanotube sensors realized on CMOS circuitry show strong response to various vapors including Dimethyl methylphosphonate and Dinitrotoluene. The remarkable set of attributes of the SWNTs realized on CMOS electronic chips provides an attractive platform for high sensitivity portable nanotube based bio and chemical sensors.
[Bio-Resources and Database for Preemptive Medicine.
Saito, Kuniaki
2016-05-01
Establishing a primary defense for the improvement of individual quality of life by epidemiology and various clinical studies applying bio-resources/database analysis is very important. Furthermore, recent studies on understanding the epigenetic regulatory mechanisms of developmental origins of health and diseases are attracting increasing interest. Therefore, the storing of not only bio-fluid (i.e., blood, urine) but also certain tissues (i.e., placenta, cord) is very important for research. The Resource Center for Health Science (RECHS) and Bio-databases Institute of Reproductive and Developmental Medicine (BIRD) have estab- lished Bio-bank and initiated a project based on the development and utilization of bio-resources/database, comprising personal health records (PHR), such as health/medical records including individual records of daily diet and exercise, physically consolidated with bio-resources, taken from the same individuals. These Bio-Resources/Database projects are very important for the establishment of preemptive medicine and un- derstanding the mechanisms of the developmental origins of health and diseases.
Bios-3: Siberian experiments in bioregenerative life support
NASA Technical Reports Server (NTRS)
Salisbury, F. B.; Gitelson, J. I.; Lisovsky, G. M.
1997-01-01
The Russian experience with the bioregenerative life support system Bios-3 at Krasnoyarsk, Siberia, is reviewed. A brief review of other bioregenerative systems examines Biosphere 2 in Oracle, Arizona, and the Bios-1 and Bios-2 systems that preceded Bios-3. Physical details of the Bios-3 facility are provided. The use of Chlorella and higher plants for gas exchange is examined. Long-term studies of human habitation are discussed. Other topics include microflora in Bios-3, the theory of closed systems, and problems for the future.
Recent Improvement of Medical Optical Fibre Pressure and Temperature Sensors.
Poeggel, Sven; Duraibabu, Dineshbabu; Kalli, Kyriacos; Leen, Gabriel; Dooly, Gerard; Lewis, Elfed; Kelly, Jimmy; Munroe, Maria
2015-07-13
This investigation describes a detailed analysis of the fabrication and testing of optical fibre pressure and temperature sensors (OFPTS). The optical sensor of this research is based on an extrinsic Fabry-Perot interferometer (EFPI) with integrated fibre Bragg grating (FBG) for simultaneous pressure and temperature measurements. The sensor is fabricated exclusively in glass and with a small diameter of 0.2 mm, making it suitable for volume-restricted bio-medical applications. Diaphragm shrinking techniques based on polishing, hydrofluoric (HF) acid and femtosecond (FS) laser micro-machining are described and analysed. The presented sensors were examined carefully and demonstrated a pressure sensitivity in the range of sp = 2-10 nm/kPa and a resolution of better than ΔP = 10 Pa protect (0.1 cm H2O). A static pressure test in 38 cm H2O shows no drift of the sensor in a six-day period. Additionally, a dynamic pressure analysis demonstrated that the OFPTS never exceeded a drift of more than 130 Pa (1.3 cm H2O) in a 12-h measurement, carried out in a cardiovascular simulator. The temperature sensitivity is given by k = 10.7 pm/K, which results in a temperature resolution of better than ΔT = 0.1 K. Since the temperature sensing element is placed close to the pressure sensing element, the pressure sensor is insensitive to temperature changes.
Recent Improvement of Medical Optical Fibre Pressure and Temperature Sensors
Poeggel, Sven; Duraibabu, Dineshbabu; Kalli, Kyriacos; Leen, Gabriel; Dooly, Gerard; Lewis, Elfed; Kelly, Jimmy; Munroe, Maria
2015-01-01
This investigation describes a detailed analysis of the fabrication and testing of optical fibre pressure and temperature sensors (OFPTS). The optical sensor of this research is based on an extrinsic Fabry–Perot interferometer (EFPI) with integrated fibre Bragg grating (FBG) for simultaneous pressure and temperature measurements. The sensor is fabricated exclusively in glass and with a small diameter of 0.2 mm, making it suitable for volume-restricted bio-medical applications. Diaphragm shrinking techniques based on polishing, hydrofluoric (HF) acid and femtosecond (FS) laser micro-machining are described and analysed. The presented sensors were examined carefully and demonstrated a pressure sensitivity in the range of sp = 2–10 nmkPa and a resolution of better than ΔP = 10 Pa (0.1 cm H2O). A static pressure test in 38 cmH2O shows no drift of the sensor in a six-day period. Additionally, a dynamic pressure analysis demonstrated that the OFPTS never exceeded a drift of more than 130 Pa (1.3 cm H2O) in a 12-h measurement, carried out in a cardiovascular simulator. The temperature sensitivity is given by k=10.7 pmK, which results in a temperature resolution of better than ΔT = 0.1 K. Since the temperature sensing element is placed close to the pressure sensing element, the pressure sensor is insensitive to temperature changes. PMID:26184331
Bio-oil and bio-char from low temperature pyrolysis of spent grains using activated alumina.
Sanna, Aimaro; Li, Sujing; Linforth, Rob; Smart, Katherine A; Andrésen, John M
2011-11-01
The pyrolysis of wheat and barley spent grains resulting from bio-ethanol and beer production respectively was investigated at temperatures between 460 and 540 °C using an activated alumina bed. The results showed that the bio-oil yield and quality depend principally on the applied temperature where pyrolysis at 460 °C leaves a bio-oil with lower nitrogen content in comparison with the original spent grains and low oxygen content. The viscosity profile of the spent grains indicated that activated alumina could promote liquefaction and prevent charring of the structure between 400 and 460 °C. The biochar contains about 10-12% of original carbon and 13-20% of starting nitrogen resulting very attractive as a soil amendment and for carbon sequestration. Overall, value can be added to the spent grains opening a new market in bio-fuel production without the needs of external energy. The bio-oil from spent grains could meet about 9% of the renewable obligation in the UK. Copyright © 2011 Elsevier Ltd. All rights reserved.
Eslaminejad, Mohammadreza; Razak, Shukor Abd
2012-01-01
Wireless sensor networks basically consist of low cost sensor nodes which collect data from environment and relay them to a sink, where they will be subsequently processed. Since wireless nodes are severely power-constrained, the major concern is how to conserve the nodes' energy so that network lifetime can be extended significantly. Employing one static sink can rapidly exhaust the energy of sink neighbors. Furthermore, using a non-optimal single path together with a maximum transmission power level may quickly deplete the energy of individual nodes on the route. This all results in unbalanced energy consumption through the sensor field, and hence a negative effect on the network lifetime. In this paper, we present a comprehensive taxonomy of the various mechanisms applied for increasing the network lifetime. These techniques, whether in the routing or cross-layer area, fall within the following types: multi-sink, mobile sink, multi-path, power control and bio-inspired algorithms, depending on the protocol operation. In this taxonomy, special attention has been devoted to the multi-sink, power control and bio-inspired algorithms, which have not yet received much consideration in the literature. Moreover, each class covers a variety of the state-of-the-art protocols, which should provide ideas for potential future works. Finally, we compare these mechanisms and discuss open research issues. PMID:23202008
Eslaminejad, Mohammadreza; Razak, Shukor Abd
2012-10-09
Wireless sensor networks basically consist of low cost sensor nodes which collect data from environment and relay them to a sink, where they will be subsequently processed. Since wireless nodes are severely power-constrained, the major concern is how to conserve the nodes' energy so that network lifetime can be extended significantly. Employing one static sink can rapidly exhaust the energy of sink neighbors. Furthermore, using a non-optimal single path together with a maximum transmission power level may quickly deplete the energy of individual nodes on the route. This all results in unbalanced energy consumption through the sensor field, and hence a negative effect on the network lifetime. In this paper, we present a comprehensive taxonomy of the various mechanisms applied for increasing the network lifetime. These techniques, whether in the routing or cross-layer area, fall within the following types: multi-sink, mobile sink, multi-path, power control and bio-inspired algorithms, depending on the protocol operation. In this taxonomy, special attention has been devoted to the multi-sink, power control and bio-inspired algorithms, which have not yet received much consideration in the literature. Moreover, each class covers a variety of the state-of-the-art protocols, which should provide ideas for potential future works. Finally, we compare these mechanisms and discuss open research issues.
[Preface for special issue on bio-based materials (2016)].
Weng, Yunxuan
2016-06-25
Bio-based materials are new materials or chemicals with renewable biomass as raw materials such as grain, legume, straw, bamboo and wood powder. This class of materials includes bio-based polymer, biobased fiber, glycotechnology products, biobased rubber and plastics produced by biomass thermoplastic processing and basic biobased chemicals, for instance, bio-alcohols, organic acids, alkanes, and alkenes, obtained by bio-synthesis, bio-processing and bio-refinery. Owing to its environmental friendly and resource conservation, bio-based materials are becoming a new dominant industry taking the lead in the world scientific and technological innovation and economic development. An overview of bio-based materials development is reported in this special issue, and the industrial status and research progress of the following aspects, including biobased fiber, polyhydroxyalkanoates, biodegradable mulching film, bio-based polyamide, protein based biomedical materials, bio-based polyurethane, and modification and processing of poly(lactic acid), are introduced.
Development of a bioaerosol single particle detector (BIO IN) for the fast ice nucleus chamber FINCH
NASA Astrophysics Data System (ADS)
Bundke, U.; Reimann, B.; Nillius, B.; Jaenicke, R.; Bingemer, H.
2009-10-01
In this work we present the setup and first tests of our new BIO IN detector. This detector is designed to classify atmospheric ice nuclei (IN) for their biological content. Biological material is identified via its auto-fluorescence (intrinsic fluorescence) after irradiation with UV radiation. Ice nuclei are key substances for precipitation development via the Bergeron-Findeisen process. The level of scientific knowledge regarding origin and climatology (temporal and spatial distribution) of IN is very low. Some biological material is known to be active as IN even at relatively high temperatures of up to -2°C (e.g. pseudomonas syringae bacteria). These biological IN could have a strong influence on the formation of clouds and precipitation. We have designed the new BIO IN sensor to analyze the abundance of IN of biological origin. The instrument will be flown on one of the first missions of the new German research aircraft ''HALO'' (High Altitude and LOng Range).
BioSearch: a semantic search engine for Bio2RDF
Qiu, Honglei; Huang, Jiacheng
2017-01-01
Abstract Biomedical data are growing at an incredible pace and require substantial expertise to organize data in a manner that makes them easily findable, accessible, interoperable and reusable. Massive effort has been devoted to using Semantic Web standards and technologies to create a network of Linked Data for the life sciences, among others. However, while these data are accessible through programmatic means, effective user interfaces for non-experts to SPARQL endpoints are few and far between. Contributing to user frustrations is that data are not necessarily described using common vocabularies, thereby making it difficult to aggregate results, especially when distributed across multiple SPARQL endpoints. We propose BioSearch — a semantic search engine that uses ontologies to enhance federated query construction and organize search results. BioSearch also features a simplified query interface that allows users to optionally filter their keywords according to classes, properties and datasets. User evaluation demonstrated that BioSearch is more effective and usable than two state of the art search and browsing solutions. Database URL: http://ws.nju.edu.cn/biosearch/ PMID:29220451
Nuzzo, Andrea; Hosseinkhani, Baharak; Boon, Nico; Zanaroli, Giulio; Fava, Fabio
2017-01-01
Biogenic palladium nanoparticles (bio-Pd NPs) represent a promising catalyst for organohalide remediation in water and sediments. However, the available information regarding their possible impact in case of release into the environment, particularly on the environmental microbiota, is limited. In this study the toxicity of bio-Pd NPs on the model marine bacterium V. fischeri was assessed. The impacts of different concentrations of bio-Pd NPs on the respiratory metabolisms (i.e. organohalide respiration, sulfate reduction and methanogenesis) and the structure of a PCB-dechlorinating microbial community enriched form a marine sediment were also investigated in microcosms mimicking the actual sampling site conditions. Bio-Pd NPs had no toxic effect on V. fischeri. In addition, they had no significant effects on PCB-dehalogenating activity, while showing a partial, dose-dependent inhibitory effect on sulfate reduction as well as on methanogenesis. No toxic effects by bio-Pd NPs could be also observed on the total bacterial community structure, as its biodiversity was increased compared to the not exposed community. In addition, resilience of the microbial community to bio-Pd NPs exposure was observed, being the final community organization (Gini coefficient) of samples exposed to bio-Pd NPs similar to that of the not exposed one. Considering all the factors evaluated, bio-Pd NPs could be deemed as non-toxic to the marine microbiota in the conditions tested. This is the first study in which the impact of bio-Pd NPs is extensively evaluated over a microbial community in relevant environmental conditions, providing important information for the assessment of their environmental safety. Copyright © 2016 Elsevier Ltd. All rights reserved.
Development of an IrO x micro pH sensor array on flexible polymer substrate
NASA Astrophysics Data System (ADS)
Huang, Wen-Ding; Wang, Jianqun; Ativanichayaphong, Thermpon; Chiao, Mu; Chiao, J. C.
2008-03-01
pH sensor is an essential component used in many chemical, food, and bio-material industries. Conventional glass electrodes have been used to construct pH sensors, however, have some disadvantages in specific applications. It is difficult to use glass electrodes for in vivo biomedical or food monitoring applications due to size limitation and no deformability. In this paper, we present design and fabrication processes of a miniature iridium oxide thin film pH sensor array on flexible polymer substrates. The amorphous iridium oxide thin film was used as the sensing material. A sol-gel dip-coating process of iridium oxide film was demonstrated in this paper. A super-Nernstian response has been measured on individual sensors of the array with a slope of -71.6+/-3 mV/pH at 25°C within the pH range between 2.83 and 11.04.
Bio-Carbon Accounting for Bio-Oil Co-Processing: 14C and 13C/ 12C
DOE Office of Scientific and Technical Information (OSTI.GOV)
Mora, Claudia I.; Li, Zhenghua; Vance, Zachary
This is a powerpoint presentation on bio-carbon accounting for bio-oil co-processing. Because of the overlapping range in the stable C isotope compositions of fossil oils and biooils from C3-type feedstocks, it is widely thought that stable isotopes are not useful to track renewable carbon during co-production. In contrast, our study demonstrates the utility of stable isotopes to: • capture a record of renewable carbon allocation between FCC products of co-processing • record changes in carbon apportionments due to changes in reactor or feed temperature Stable isotope trends as a function of percent bio-oil in the feed are more pronounced whenmore » the δ 13C of the bio-oil endmember differs greatly from the VGO (i.e., it has a C4 biomass source–corn stover, switch grass, Miscanthus, sugarcane– versus a C3 biomass source– pine, wheat, rice, potato), but trends on the latter case are significant for endmember differences of just a few permil. The correlation between measured 14C and δ 13C may be useful as an alternative to carbon accounting, but the relationship must first be established for different bio-oil sources.« less
Composition and parameters of household bio-waste in four seasons.
Hanc, Ales; Novak, Pavel; Dvorak, Milan; Habart, Jan; Svehla, Pavel
2011-07-01
Bio-waste makes up almost half portion of municipal solid waste. The characterization of household bio-waste is important in determining the most appropriate treatment method. The differences in composition and parameters of bio-waste derived from urban settlement (U-bio-waste) and family houses (F-bio-waste) during the four climate seasons are described in this paper. Twelve components and 20 parameters for bio-waste were evaluated. The composition of U-bio-waste was almost steady over those seasons, unlike F-bio-waste. U-bio-waste was comprised mainly (58.2%) of fruit and vegetable debris. F-bio-waste was primarily made up of seasonal garden components. The amount of variation among seasons in both type of bio-waste increased in sequence: basic parameters
Green bio-oil extraction for oil crops
NASA Astrophysics Data System (ADS)
Zainab, H.; Nurfatirah, N.; Norfaezah, A.; Othman, H.
2016-06-01
The move towards a green bio-oil extraction technique is highlighted in this paper. The commonly practised organic solvent oil extraction technique could be replaced with a modified microwave extraction. Jatropha seeds (Jatropha curcas) were used to extract bio-oil. Clean samples were heated in an oven at 110 ° C for 24 hours to remove moisture content and ground to obtain particle size smaller than 500μm. Extraction was carried out at different extraction times 15 min, 30 min, 45 min, 60 min and 120 min to determine oil yield. The biooil yield obtained from microwave assisted extraction system at 90 minutes was 36% while that from soxhlet extraction for 6 hours was 42%. Bio-oil extracted using the microwave assisted extraction (MAE) system could enhance yield of bio-oil compared to soxhlet extraction. The MAE extraction system is rapid using only water as solvent which is a nonhazardous, environment-friendly technique compared to soxhlet extraction (SE) method using hexane as solvent. Thus, this is a green technique of bio-oil extraction using only water as extractant. Bio-oil extraction from the pyrolysis of empty fruit bunch (EFB), a biomass waste from oil palm crop, was enhanced using a biocatalyst derived from seashell waste. Oil yield for non-catalytic extraction was 43.8% while addition of seashell based biocatalyst was 44.6%. Oil yield for non-catalytic extraction was 43.8% while with addition of seashell-based biocatalyst was 44.6%. The pH of bio-oil increased from 3.5 to 4.3. The viscosity of bio-oil obtained by catalytic means increased from 20.5 to 37.8 cP. A rapid and environment friendly extraction technique is preferable to enhance bio-oil yield. The microwave assisted approach is a green, rapid and environmental friendly extraction technique for the production of bio-oil bearing crops.
BIO ARGO floats: tools for operational monitoring of the Black Sea
NASA Astrophysics Data System (ADS)
Palazov, Atanas; Slabakova, Violeta; Peneva, Elisaveta; Stanev, Emil
2014-05-01
The assessment of ecological status in the context of the Water Framework Directive (WFD) and Marine Strategy Framework Directive (MSFD) requires comprehensive knowledge and understanding of the physical and biogeochemical processes that determine the functioning of marine ecosystems. One of the main challenges however is the need of data with frequency relevant to the spatial and temporal scales of the ecological processes. The majority of in situ observations that are commonly used for ecological monitoring of the Black Sea are generally based on near-shore monitoring programs or irregular oceanographic cruises that provide either non-synoptic, coarse resolution realizations of large scale processes or detailed, but time and site specific snapshots of local features. These gaps can be filled by two independent sources: satellite observation and profiling floats. In fact satellite ocean color sensors allows for determination at synoptic scale of water quality parameters through its absorption properties. However the satellite ocean color methods have a number of limitations such as: measurements can only be made during daylight hours; require cloud-free conditions and are sensitive to atmospheric aerosols; provide information only for the upper layer of the ocean (approximately the depth of 10% incident light); algorithms developed for global applications are a source of large uncertainties in the marginal seas and costal areas. These constrains of the optical remote sensing observations can be avoided by using miniature biogeochemical sensors and autonomous platforms that offer remarkable perspectives for observing the "biological" ocean, notably at critical spatiotemporal scales which have been out of reach until recently (Claustre et al., 2010). In the frame of "E-AIMS: Euro-Argo Improvements for the GMES marine Service" 7 EC FP project two Bio Argo floats were deployed in the Black Sea. Beside the traditionally CTD the floats were equipped with biogeochemical
BIO::Phylo-phyloinformatic analysis using perl.
Vos, Rutger A; Caravas, Jason; Hartmann, Klaas; Jensen, Mark A; Miller, Chase
2011-02-27
Phyloinformatic analyses involve large amounts of data and metadata of complex structure. Collecting, processing, analyzing, visualizing and summarizing these data and metadata should be done in steps that can be automated and reproduced. This requires flexible, modular toolkits that can represent, manipulate and persist phylogenetic data and metadata as objects with programmable interfaces. This paper presents Bio::Phylo, a Perl5 toolkit for phyloinformatic analysis. It implements classes and methods that are compatible with the well-known BioPerl toolkit, but is independent from it (making it easy to install) and features a richer API and a data model that is better able to manage the complex relationships between different fundamental data and metadata objects in phylogenetics. It supports commonly used file formats for phylogenetic data including the novel NeXML standard, which allows rich annotations of phylogenetic data to be stored and shared. Bio::Phylo can interact with BioPerl, thereby giving access to the file formats that BioPerl supports. Many methods for data simulation, transformation and manipulation, the analysis of tree shape, and tree visualization are provided. Bio::Phylo is composed of 59 richly documented Perl5 modules. It has been deployed successfully on a variety of computer architectures (including various Linux distributions, Mac OS X versions, Windows, Cygwin and UNIX-like systems). It is available as open source (GPL) software from http://search.cpan.org/dist/Bio-Phylo.
BIO::Phylo-phyloinformatic analysis using perl
2011-01-01
Background Phyloinformatic analyses involve large amounts of data and metadata of complex structure. Collecting, processing, analyzing, visualizing and summarizing these data and metadata should be done in steps that can be automated and reproduced. This requires flexible, modular toolkits that can represent, manipulate and persist phylogenetic data and metadata as objects with programmable interfaces. Results This paper presents Bio::Phylo, a Perl5 toolkit for phyloinformatic analysis. It implements classes and methods that are compatible with the well-known BioPerl toolkit, but is independent from it (making it easy to install) and features a richer API and a data model that is better able to manage the complex relationships between different fundamental data and metadata objects in phylogenetics. It supports commonly used file formats for phylogenetic data including the novel NeXML standard, which allows rich annotations of phylogenetic data to be stored and shared. Bio::Phylo can interact with BioPerl, thereby giving access to the file formats that BioPerl supports. Many methods for data simulation, transformation and manipulation, the analysis of tree shape, and tree visualization are provided. Conclusions Bio::Phylo is composed of 59 richly documented Perl5 modules. It has been deployed successfully on a variety of computer architectures (including various Linux distributions, Mac OS X versions, Windows, Cygwin and UNIX-like systems). It is available as open source (GPL) software from http://search.cpan.org/dist/Bio-Phylo PMID:21352572
Bio-TDS: bioscience query tool discovery system.
Gnimpieba, Etienne Z; VanDiermen, Menno S; Gustafson, Shayla M; Conn, Bill; Lushbough, Carol M
2017-01-04
Bioinformatics and computational biology play a critical role in bioscience and biomedical research. As researchers design their experimental projects, one major challenge is to find the most relevant bioinformatics toolkits that will lead to new knowledge discovery from their data. The Bio-TDS (Bioscience Query Tool Discovery Systems, http://biotds.org/) has been developed to assist researchers in retrieving the most applicable analytic tools by allowing them to formulate their questions as free text. The Bio-TDS is a flexible retrieval system that affords users from multiple bioscience domains (e.g. genomic, proteomic, bio-imaging) the ability to query over 12 000 analytic tool descriptions integrated from well-established, community repositories. One of the primary components of the Bio-TDS is the ontology and natural language processing workflow for annotation, curation, query processing, and evaluation. The Bio-TDS's scientific impact was evaluated using sample questions posed by researchers retrieved from Biostars, a site focusing on BIOLOGICAL DATA ANALYSIS: The Bio-TDS was compared to five similar bioscience analytic tool retrieval systems with the Bio-TDS outperforming the others in terms of relevance and completeness. The Bio-TDS offers researchers the capacity to associate their bioscience question with the most relevant computational toolsets required for the data analysis in their knowledge discovery process. © The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.
Blood pulse wave velocity and pressure sensing via fiber based and free space based optical sensors
NASA Astrophysics Data System (ADS)
Sirkis, Talia; Beiderman, Yevgeny; Agdarov, Sergey; Beiderman, Yafim; Zalevsky, Zeev
2017-02-01
Continuous noninvasive measurement of vital bio-signs, such as cardiopulmonary parameters, is an important tool in evaluation of the patient's physiological condition and health monitoring. On the demand of new enabling technologies, some works have been done in continuous monitoring of blood pressure and pulse wave velocity. In this paper, we introduce two techniques for non-contact sensing of vital bio signs. In the first approach the optical sensor is based on single mode in-fibers Mach-Zehnder interferometer (MZI) to detect heartbeat, respiration and pulse wave velocity (PWV). The introduced interferometer is based on a new implanted scheme. It replaces the conventional MZI realized by inserting of discontinuities in the fiber to break the total internal reflection and scatter/collect light. The proposed fiber sensor was successfully incorporated into shirt to produce smart clothing. The measurements obtained from the smart clothing could be obtained in comfortable manner and there is no need to have an initial calibration or a direct contact between the sensor and the skin of the tested individual. In the second concept we show a remote noncontact blood pulse wave velocity and pressure measurement based on tracking the temporal changes of reflected secondary speckle patterns produced in human skin when illuminated by a laser beams. In both concept experimental validation of the proposed schemes is shown and analyzed.
The Problem of Bio-Concepts: Biopolitics, Bio-Economy and the Political Economy of Nothing
ERIC Educational Resources Information Center
Birch, Kean
2017-01-01
Scholars in science and technology studies--and no doubt other fields--have increasingly drawn on Michel Foucault's concept of biopolitics to theorize a variety of new "bio-concepts." While there might be some theoretical value in such exercises, many of these bio-concepts have simply replaced more rigorous--and therefore…
Development of a bio-magnetic measurement system and sensor configuration analysis for rats
NASA Astrophysics Data System (ADS)
Kim, Ji-Eun; Kim, In-Seon; Kim, Kiwoong; Lim, Sanghyun; Kwon, Hyukchan; Kang, Chan Seok; Ahn, San; Yu, Kwon Kyu; Lee, Yong-Ho
2017-04-01
Magnetoencephalography (MEG) based on superconducting quantum interference devices enables the measurement of very weak magnetic fields (10-1000 fT) generated from the human or animal brain. In this article, we introduce a small MEG system that we developed specifically for use with rats. Our system has the following characteristics: (1) variable distance between the pick-up coil and outer Dewar bottom (˜5 mm), (2) small pick-up coil (4 mm) for high spatial resolution, (3) good field sensitivity (45 ˜ 80 fT /cm/√{Hz} ) , (4) the sensor interval satisfies the Nyquist spatial sampling theorem, and (5) small source localization error for the region to be investigated. To reduce source localization error, it is necessary to establish an optimal sensor layout. To this end, we simulated confidence volumes at each point on a grid on the surface of a virtual rat head. In this simulation, we used locally fitted spheres as model rat heads. This enabled us to consider more realistic volume currents. We constrained the model such that the dipoles could have only four possible orientations: the x- and y-axes from the original coordinates, and two tangentially layered dipoles (local x- and y-axes) in the locally fitted spheres. We considered the confidence volumes according to the sensor layout and dipole orientation and positions. We then conducted a preliminary test with a 4-channel MEG system prior to manufacturing the multi-channel system. Using the 4-channel MEG system, we measured rat magnetocardiograms. We obtained well defined P-, QRS-, and T-waves in rats with a maximum value of 15 pT/cm. Finally, we measured auditory evoked fields and steady state auditory evoked fields with maximum values 400 fT/cm and 250 fT/cm, respectively.
NASA Astrophysics Data System (ADS)
Du, Xianbin
2018-01-01
Ultimate goal of tissue engineering is to replace pathological or necrotic body tissue or organ by artificial tissue or organ and tissue engineering is a very promising research field. 3D bio-printing is a kind of emerging technologies and a branch of tissue engineering. It has made significant progress in the past decade. 3D bio-printing can realize tissue and organ construction in vitro and has wide application in basic research and pharmacy. This paper is to make an analysis and review on 3D bio-printing from the perspectives of bioink, printing technology and technology application.
Bio-SimVerb and Bio-SimLex: wide-coverage evaluation sets of word similarity in biomedicine.
Chiu, Billy; Pyysalo, Sampo; Vulić, Ivan; Korhonen, Anna
2018-02-05
Word representations support a variety of Natural Language Processing (NLP) tasks. The quality of these representations is typically assessed by comparing the distances in the induced vector spaces against human similarity judgements. Whereas comprehensive evaluation resources have recently been developed for the general domain, similar resources for biomedicine currently suffer from the lack of coverage, both in terms of word types included and with respect to the semantic distinctions. Notably, verbs have been excluded, although they are essential for the interpretation of biomedical language. Further, current resources do not discern between semantic similarity and semantic relatedness, although this has been proven as an important predictor of the usefulness of word representations and their performance in downstream applications. We present two novel comprehensive resources targeting the evaluation of word representations in biomedicine. These resources, Bio-SimVerb and Bio-SimLex, address the previously mentioned problems, and can be used for evaluations of verb and noun representations respectively. In our experiments, we have computed the Pearson's correlation between performances on intrinsic and extrinsic tasks using twelve popular state-of-the-art representation models (e.g. word2vec models). The intrinsic-extrinsic correlations using our datasets are notably higher than with previous intrinsic evaluation benchmarks such as UMNSRS and MayoSRS. In addition, when evaluating representation models for their abilities to capture verb and noun semantics individually, we show a considerable variation between performances across all models. Bio-SimVerb and Bio-SimLex enable intrinsic evaluation of word representations. This evaluation can serve as a predictor of performance on various downstream tasks in the biomedical domain. The results on Bio-SimVerb and Bio-SimLex using standard word representation models highlight the importance of developing dedicated
The Bio-Community Perl toolkit for microbial ecology.
Angly, Florent E; Fields, Christopher J; Tyson, Gene W
2014-07-01
The development of bioinformatic solutions for microbial ecology in Perl is limited by the lack of modules to represent and manipulate microbial community profiles from amplicon and meta-omics studies. Here we introduce Bio-Community, an open-source, collaborative toolkit that extends BioPerl. Bio-Community interfaces with commonly used programs using various file formats, including BIOM, and provides operations such as rarefaction and taxonomic summaries. Bio-Community will help bioinformaticians to quickly piece together custom analysis pipelines and develop novel software. Availability an implementation: Bio-Community is cross-platform Perl code available from http://search.cpan.org/dist/Bio-Community under the Perl license. A readme file describes software installation and how to contribute. © The Author 2014. Published by Oxford University Press.
Querying and Computing with BioCyc Databases
Krummenacker, Markus; Paley, Suzanne; Mueller, Lukas; Yan, Thomas; Karp, Peter D.
2006-01-01
Summary We describe multiple methods for accessing and querying the complex and integrated cellular data in the BioCyc family of databases: access through multiple file formats, access through Application Program Interfaces (APIs) for LISP, Perl and Java, and SQL access through the BioWarehouse relational database. Availability The Pathway Tools software and 20 BioCyc DBs in Tiers 1 and 2 are freely available to academic users; fees apply to some types of commercial use. For download instructions see http://BioCyc.org/download.shtml PMID:15961440
Cost effective spectral sensor solutions for hand held and field applications
NASA Astrophysics Data System (ADS)
Reetz, Edgar; Correns, Martin; Notni, Gunther
2015-05-01
Optical spectroscopy is without doubt one of the most important non-contact measurement principles. It is used in a wide range of applications from bio-medical to industrial fields. One recent trend is to miniaturize spectral sensors to address new areas of application. The most common spectral sensor type is based on diffraction gratings, while other types are based on micro mechanical systems (MEMS) or filter technologies. The authors represent the opinion that there is a potentially wide spread field of applications for spectrometers, but the market limits the range of applications since they cannot keep up with targeted cost requirements for consumer products. The present article explains an alternative approach for miniature multichannel spectrometer to enhance robustness for hand held field applications at a cost efficient price point.
NASA Astrophysics Data System (ADS)
Choi, Haecheon
2009-11-01
Bio-mimetic engineering or bio-mimetics is the application of biological methods and systems found in nature to the study and design of engineering systems and modern technology (from Wikipedia). The concept itself is old, but successful developments have been made recently, especially in the research field of flow control. The objective of flow control based on the bio-mimetic approach is to develop novel concepts for reducing drag, increasing lift and enhancing aerodynamic performance. For skin friction reduction, a few ideas have been suggested such as the riblet from shark, compliant surface from dolphin, microbubble injection and multiple front-body curvature from penguin, and V-shaped protrusion from sailfish. For form drag reduction, several new attempts have been also made recently. Examples include the V-shaped spanwise grooves from saguaro cactus, overall shape of box fish, longitudinal grooves on scallop shell, bill of swordfish, hooked comb on owl wing, trailing-edge protrusion on dragonfly wing, and fillet. For the enhancement of aerodynamic performance, focuses have been made on the birds, fish and insects: e.g., double layered feather of landing bird, leading-edge serration of humpback-whale flipper, pectoral fin of flying fish, long tail on swallowtail-butterfly wing, wing flapping motion of dragonfly, and alula in birds. Living animals adapt their bodies to better performance in multi purposes, but engineering requires single purpose in most cases. Therefore, bio-mimetic approaches often produce excellent results more than expected. However, they are sometimes based on people's wrong understanding of nature and produce unwanted results. Successes and failures from bio-mimetic approaches in flow control will be discussed in the presentation.
Development of Implantable Wireless Sensor Nodes for Animal Husbandry and MedTech Innovation
Lu, Jian; Zhang, Lan; Zhang, Dapeng; Matsumoto, Sohei; Hiroshima, Hiroshi; Maeda, Ryutaro; Sato, Mizuho; Toyoda, Atsushi; Gotoh, Takafumi; Ohkohchi, Nobuhiro
2018-01-01
In this paper, we report the development, evaluation, and application of ultra-small low-power wireless sensor nodes for advancing animal husbandry, as well as for innovation of medical technologies. A radio frequency identification (RFID) chip with hybrid interface and neglectable power consumption was introduced to enable switching of ON/OFF and measurement mode after implantation. A wireless power transmission system with a maximum efficiency of 70% and an access distance of up to 5 cm was developed to allow the sensor node to survive for a duration of several weeks from a few minutes’ remote charge. The results of field tests using laboratory mice and a cow indicated the high accuracy of the collected biological data and bio-compatibility of the package. As a result of extensive application of the above technologies, a fully solid wireless pH sensor and a surgical navigation system using artificial magnetic field and a 3D MEMS magnetic sensor are introduced in this paper, and the preliminary experimental results are presented and discussed. PMID:29587448
Development of Implantable Wireless Sensor Nodes for Animal Husbandry and MedTech Innovation.
Lu, Jian; Zhang, Lan; Zhang, Dapeng; Matsumoto, Sohei; Hiroshima, Hiroshi; Maeda, Ryutaro; Sato, Mizuho; Toyoda, Atsushi; Gotoh, Takafumi; Ohkohchi, Nobuhiro
2018-03-26
In this paper, we report the development, evaluation, and application of ultra-small low-power wireless sensor nodes for advancing animal husbandry, as well as for innovation of medical technologies. A radio frequency identification (RFID) chip with hybrid interface and neglectable power consumption was introduced to enable switching of ON/OFF and measurement mode after implantation. A wireless power transmission system with a maximum efficiency of 70% and an access distance of up to 5 cm was developed to allow the sensor node to survive for a duration of several weeks from a few minutes' remote charge. The results of field tests using laboratory mice and a cow indicated the high accuracy of the collected biological data and bio-compatibility of the package. As a result of extensive application of the above technologies, a fully solid wireless pH sensor and a surgical navigation system using artificial magnetic field and a 3D MEMS magnetic sensor are introduced in this paper, and the preliminary experimental results are presented and discussed.
Past, Present, and Future Production of Bio-oil
DOE Office of Scientific and Technical Information (OSTI.GOV)
Steele, Philip; Yu, Fei; Gajjela, Sanjeev
Bio-oil is a liquid product produced by fast pyrol-ysis of biomass. The fast pyrolysis is performed by heating the biomass rapidly (2 sec) at temperatures ranging from 350 to 650 oC. The vapors produced by this rapid heating are then condensed to produce a dark brown water-based emulsion composed of frag-ments of the original hemicellulose, cellulose and lignin molecules contained in the biomass. Yields range from 60 to 75% based on the feedstock type and the pyrolysis reactor employed. The bio-oil pro-duced by this process has a number of negative prop-erties that are produced mainly by the high oxygen contentmore » (40 to 50%) contributed by that contained in water (25 to 30% of total mass) and oxygenated compounds. Each bio-oil contains hundreds of chemi-cal compounds. The chemical composition of bio-oil renders it a very recalcitrant chemical compound. To date, the difficulties in utilizing bio-oil have limited its commercial development to the production of liq-uid smoke as food flavoring. Practitioners have at-tempted to utilize raw bio-oil as a fuel; they have also applied many techniques to upgrade bio-oil to a fuel. Attempts to utilize raw bio-oil as a combustion engine fuel have resulted in engine or turbine dam-age; however, Stirling engines have been shown to successfully combust raw bio-oil without damage. Utilization of raw bio-oil as a boiler fuel has met with more success and an ASTM standard has recently been released describing bio-oil characteristics in relation to assigned fuel grades. However, commercialization has been slow to follow and no reports of distribution of these bio-oil boiler fuels have been reported. Co-feeding raw bio-oil with coal has been successfully performed but no current power generation facilities are following this practice. Upgrading of bio-oils to hydrocarbons via hydroprocessing is being performed by several organizations. Currently, limited catalyst life is the obstacle to commercialization of this tech
BioC implementations in Go, Perl, Python and Ruby
Liu, Wanli; Islamaj Doğan, Rezarta; Kwon, Dongseop; Marques, Hernani; Rinaldi, Fabio; Wilbur, W. John; Comeau, Donald C.
2014-01-01
As part of a communitywide effort for evaluating text mining and information extraction systems applied to the biomedical domain, BioC is focused on the goal of interoperability, currently a major barrier to wide-scale adoption of text mining tools. BioC is a simple XML format, specified by DTD, for exchanging data for biomedical natural language processing. With initial implementations in C++ and Java, BioC provides libraries of code for reading and writing BioC text documents and annotations. We extend BioC to Perl, Python, Go and Ruby. We used SWIG to extend the C++ implementation for Perl and one Python implementation. A second Python implementation and the Ruby implementation use native data structures and libraries. BioC is also implemented in the Google language Go. BioC modules are functional in all of these languages, which can facilitate text mining tasks. BioC implementations are freely available through the BioC site: http://bioc.sourceforge.net. Database URL: http://bioc.sourceforge.net/ PMID:24961236
NASA Astrophysics Data System (ADS)
Al-abbas, Mustafa Hamid; Ibrahim, Wan Aini Wan; Sanagi, Mohd. Marsin
2012-09-01
Recycling waste materials produced in our daily life is considered as an additional resource of a wide range of materials and it conserves the environment. Used engine oil and used cooking oil are two oils disposed off in large quantities as a by-product of our daily life. This study aims at providing white bio oil, bio petroleum diesel and heavy fuel from the disposed oils. Toxic organic materials suspected to be present in the used engine oil were separated using vacuum column chromatography to reduce the time needed for the separation process and to avoid solvent usage. The compounds separated were detected by gas chromatography-mass spectrometry (GC-MS) and found to contain toxic aromatic carboxylic acids. Used cooking oils (thermally cracked from usage) were collected and separated by vacuum column chromatography. White bio oil produced was examined by GC-MS. The white bio oil consists of non-toxic hydrocarbons and is found to be a good alternative to white mineral oil which is significantly used in food industry, cosmetics and drugs with the risk of containing polycyclic aromatic compounds which are carcinogenic and toxic. Different portions of the used cooking oil and used engine were mixed to produce several blends for use as heavy oil fuels. White bio oil was used to produce bio petroleum diesel by blending it with petroleum diesel and kerosene. The bio petroleum diesel produced passed the PETRONAS flash point and viscosity specification test. The heat of combustion of the two blends of heavy fuel produced was measured and one of the blends was burned to demonstrate its burning ability. Higher heat of combustion was obtained from the blend containing greater proportion of used engine oil. This study has provided a successful recycled alternative for white bio oil, bio petroleum fuel and diesel which can be an energy source.
Core ethical values: EuropaBio.
2002-01-01
EuropaBio, the European Association for BioIndustries, represents 40 companies operating world wide and 14 national association (totaling around 600 small and medium-sized enterprises) involved in the research, development, testing, manufacturing, marketing, sales and distribution of biotechnology products and services in the fields of healthcare, agriculture, food and the environment.
Torri, Cristian; Samorì, Chiara; Adamiano, Alessio; Fabbri, Daniele; Faraloni, Cecilia; Torzillo, Giuseppe
2011-09-01
The aim of this work was to investigate the potential conversion of Chlamydomonas reinhardtii biomass harvested after hydrogen production. The spent algal biomass was converted into nitrogen-rich bio-char, biodiesel and pyrolysis oil (bio-oil). The yield of lipids (algal oil), obtained by solvent extraction, was 15 ± 2% w/w(dry-biomass). This oil was converted into biodiesel with a 8.7 ± 1% w/w(dry-biomass) yield. The extraction residue was pyrolysed in a fixed bed reactor at 350 °C obtaining bio-char as the principal fraction (44 ± 1% w/w(dry-biomass)) and 28 ± 2% w/w(dry-biomass) of bio-oil. Pyrolysis fractions were characterized by elemental analysis, while the chemical composition of bio-oil was fully characterized by GC-MS, using various derivatization techniques. Energy outputs resulting from this approach were distributed in hydrogen (40%), biodiesel (12%) and pyrolysis fractions (48%), whereas bio-char was the largest fraction in terms of mass. Copyright © 2011 Elsevier Ltd. All rights reserved.
[Development and perspective of bio-based chemical fiber industry].
Li, Zengjun
2016-06-25
Bio-based fiber is environment friendly, reproducible, easily biodegradable. Therefore, rapid development of bio-based fiber industry is an obvious in progress to replace petrochemical resources, develop sustainable economy, build resource saving and environment friendly society. This article describes the current development of bio-based fiber industry, analyzes existing problems, indicates the trends and objectives of bio-based fiber materials technology innovation and recommends developing bio-based fibers industry of our country.
Norouzi, Omid; Jafarian, Sajedeh; Safari, Farid; Tavasoli, Ahmad; Nejati, Behnam
2016-11-01
Conversion of Cladophora glomerata (C. glomerata) as a Caspian Sea's green macroalgae into gaseous, liquid and solid products was carried out via pyrolysis at different temperatures to determine its potential for bio-oil and hydrogen-rich gas production for further industrial utilization. Non-catalytic tests were performed to determine the optimum condition for bio-oil production. The highest portion of bio-oil was retrieved at 500°C. The catalytic test was performed using the bio-char derived at 500°C as a catalyst. Effect of the addition of the algal bio-char on the composition of the bio-oil and also gaseous products was investigated. Pyrolysis derived bio-char was characterized by BET, FESEM and ICP method to show its surface area, porosity, and presence of inorganic metals on its surface, respectively. Phenols were increased from 8.5 to 20.76area% by the addition of bio-char. Moreover, the hydrogen concentration and hydrogen selectivity were also enhanced by the factors of 1.37, 1.59 respectively. Copyright © 2016 Elsevier Ltd. All rights reserved.
Plasmonic Refractive Index Sensor with High Figure of Merit Based on Concentric-Rings Resonator
Zhang, Zhaojian; Yang, Junbo; He, Xin; Zhang, Jingjing; Huang, Jie; Chen, Dingbo; Han, Yunxin
2018-01-01
A plasmonic refractive index (RI) sensor based on metal-insulator-metal (MIM) waveguide coupled with concentric double rings resonator (CDRR) is proposed and investigated numerically. Utilizing the novel supermodes of the CDRR, the FWHM of the resonant wavelength can be modulated, and a sensitivity of 1060 nm/RIU with high figure of merit (FOM) 203.8 is realized in the near-infrared region. The unordinary modes, as well as the influence of structure parameters on the sensing performance, are also discussed. Such plasmonic sensor with simple framework and high optical resolution could be applied to on-chip sensing systems and integrated optical circuits. Besides, the special cases of bio-sensing and triple rings are also discussed. PMID:29300331
Non-Invasive Electromagnetic Skin Patch Sensor to Measure Intracranial Fluid–Volume Shifts
Griffith, Jacob; Cluff, Kim; Eckerman, Brandon; Aldrich, Jessica; Becker, Ryan; Moore-Jansen, Peer; Patterson, Jeremy
2018-01-01
Elevated intracranial fluid volume can drive intracranial pressure increases, which can potentially result in numerous neurological complications or death. This study’s focus was to develop a passive skin patch sensor for the head that would non-invasively measure cranial fluid volume shifts. The sensor consists of a single baseline component configured into a rectangular planar spiral with a self-resonant frequency response when impinged upon by external radio frequency sweeps. Fluid volume changes (10 mL increments) were detected through cranial bone using the sensor on a dry human skull model. Preliminary human tests utilized two sensors to determine feasibility of detecting fluid volume shifts in the complex environment of the human body. The correlation between fluid volume changes and shifts in the first resonance frequency using the dry human skull was classified as a second order polynomial with R2 = 0.97. During preliminary and secondary human tests, a ≈24 MHz and an average of ≈45.07 MHz shifts in the principal resonant frequency were measured respectively, corresponding to the induced cephalad bio-fluid shifts. This electromagnetic resonant sensor may provide a non-invasive method to monitor shifts in fluid volume and assist with medical scenarios including stroke, cerebral hemorrhage, concussion, or monitoring intracranial pressure. PMID:29596338
The BioGRID interaction database: 2013 update.
Chatr-Aryamontri, Andrew; Breitkreutz, Bobby-Joe; Heinicke, Sven; Boucher, Lorrie; Winter, Andrew; Stark, Chris; Nixon, Julie; Ramage, Lindsay; Kolas, Nadine; O'Donnell, Lara; Reguly, Teresa; Breitkreutz, Ashton; Sellam, Adnane; Chen, Daici; Chang, Christie; Rust, Jennifer; Livstone, Michael; Oughtred, Rose; Dolinski, Kara; Tyers, Mike
2013-01-01
The Biological General Repository for Interaction Datasets (BioGRID: http//thebiogrid.org) is an open access archive of genetic and protein interactions that are curated from the primary biomedical literature for all major model organism species. As of September 2012, BioGRID houses more than 500 000 manually annotated interactions from more than 30 model organisms. BioGRID maintains complete curation coverage of the literature for the budding yeast Saccharomyces cerevisiae, the fission yeast Schizosaccharomyces pombe and the model plant Arabidopsis thaliana. A number of themed curation projects in areas of biomedical importance are also supported. BioGRID has established collaborations and/or shares data records for the annotation of interactions and phenotypes with most major model organism databases, including Saccharomyces Genome Database, PomBase, WormBase, FlyBase and The Arabidopsis Information Resource. BioGRID also actively engages with the text-mining community to benchmark and deploy automated tools to expedite curation workflows. BioGRID data are freely accessible through both a user-defined interactive interface and in batch downloads in a wide variety of formats, including PSI-MI2.5 and tab-delimited files. BioGRID records can also be interrogated and analyzed with a series of new bioinformatics tools, which include a post-translational modification viewer, a graphical viewer, a REST service and a Cytoscape plugin.
Rahman, Mohammed M.
2014-01-01
A reusable and mediator-free cholesterol biosensor based on cholesterol oxidase (ChOx) was fabricated based on self-assembled monolayer (SAM) of thioglycolic acid (TGA) (covalent enzyme immobilization by dropping method) using bio-chips. Cholesterol was detected with modified bio-chip (Gold/Thioglycolic-acid/Cholesterol-oxidase i.e., Au/TGA/ChOx) by reliable cyclic voltammetric (CV) technique at room conditions. The Au/TGA/ChOx modified bio-chip sensor demonstrates good linearity (1.0 nM to 1.0 mM; R = 0.9935), low-detection limit (∼0.42 nM, SNR∼3), and higher sensitivity (∼74.3 µAµM−1cm−2), lowest-small sample volume (50.0 μL), good stability, and reproducibility. To the best of our knowledge, this is the first statement with a very high sensitivity, low-detection limit, and low-sample volumes are required for cholesterol biosensor using Au/TGA/ChOx-chips assembly. The result of this facile approach was investigated for the biomedical applications for real samples at room conditions with significant assembly (Au/TGA/ChOx) towards the development of selected cholesterol biosensors, which can offer analytical access to a large group of enzymes for wide range of biomedical applications in health-care fields. PMID:24949733
M13 Bacteriophage Based Protein Sensors
NASA Astrophysics Data System (ADS)
Lee, Ju Hun
Despite significant progress in biotechnology and biosensing, early detection and disease diagnosis remains a critical issue for improving patient survival rates and well-being. Many of the typical detection schemes currently used possess issues such as low sensitivity and accuracy and are also time consuming to run and expensive. In addition, multiplexed detection remains difficult to achieve. Therefore, developing advanced approaches for reliable, simple, quantitative analysis of multiple markers in solution that also are highly sensitive are still in demand. In recent years, much of the research has primarily focused on improving two key components of biosensors: the bio-recognition agent (bio-receptor) and the transducer. Particular bio-receptors that have been used include antibodies, aptamers, molecular imprinted polymers, and small affinity peptides. In terms of transducing agents, nanomaterials have been considered as attractive candidates due to their inherent nanoscale size, durability and unique chemical and physical properties. The key focus of this thesis is the design of a protein detection and identification system that is based on chemically engineered M13 bacteriophage coupled with nanomaterials. The first chapter provides an introduction of biosensors and M13 bacteriophage in general, where the advantages of each are provided. In chapter 2, an efficient and enzyme-free sensor is demonstrated from modified M13 bacteriophage to generate highly sensitive colorimetric signals from gold nanocrystals. In chapter 3, DNA conjugated M13 were used to enable facile and rapid detection of antigens in solution that also provides modalities for identification. Lastly, high DNA loadings per phage was achieved via hydrozone chemistry and these were applied in conjunction with Raman active DNA-gold/silver core/shell nanoparticles toward highly sensitive SERS sensing.
BioNet Digital Communications Framework
NASA Technical Reports Server (NTRS)
Gifford, Kevin; Kuzminsky, Sebastian; Williams, Shea
2010-01-01
BioNet v2 is a peer-to-peer middleware that enables digital communication devices to talk to each other. It provides a software development framework, standardized application, network-transparent device integration services, a flexible messaging model, and network communications for distributed applications. BioNet is an implementation of the Constellation Program Command, Control, Communications and Information (C3I) Interoperability specification, given in CxP 70022-01. The system architecture provides the necessary infrastructure for the integration of heterogeneous wired and wireless sensing and control devices into a unified data system with a standardized application interface, providing plug-and-play operation for hardware and software systems. BioNet v2 features a naming schema for mobility and coarse-grained localization information, data normalization within a network-transparent device driver framework, enabling of network communications to non-IP devices, and fine-grained application control of data subscription band width usage. BioNet directly integrates Disruption Tolerant Networking (DTN) as a communications technology, enabling networked communications with assets that are only intermittently connected including orbiting relay satellites and planetary rover vehicles.
NASA Astrophysics Data System (ADS)
Xia, Xiaoyuan; Zhang, Zhixiang; Li, Xinxin
2008-03-01
Second torsion-mode resonance is proposed for microcantilever biosensors for ultra-high mass-weighing sensitivity and resolution. By increasing both the resonant frequency and Q-factor, the higher mode torsional resonance is favorable for improving the mass-sensing performance. For the first time, a Latin-cross-shaped second-mode resonant cantilever is constructed and optimally designed for both signal-readout and resonance-exciting elements. The cantilever sensor is fabricated by using silicon micromachining techniques. The transverse piezoresistive sensing element and the specific-shaped resonance-exciting loop are successfully integrated in the cantilever. Alpha-fetoprotein (AFP) antibody-antigen specific binding is implemented for the sensing experiment. The proposed cantilever sensor is designed with significantly superior sensitivity to the previously reported first torsion-mode one. After analysis with an Allan variance algorithm, which can be easily embedded in the sensing system, the Latin-cross-shaped second torsion-mode resonant cantilever is evaluated with ultra-high mass resolution. Therefore, the high-performance integrated micro-sensor is promising for on-the-spot bio-molecule detection.
2008-04-17
DEHULL, FLAKE HEXANE EXTRACTION PRESS HEXANE DISTILLATION CRUDE OIL 0.1-3% phosphatides 1% fatty acids 1 ppm chlorophyll DEGUM (H2 O, H3 PO4...program www.bfrl.nist.gov/oae/bees.html 617 April 2008 Seed Oils and Their Fatty Acid and Genetic Varieties Source: Leissner, O. et al (1989) Vegetable...Oils and Fats, Karlshammn, Sweden 717 April 2008 Bio-based Oil Process Volatile impurities: odor (aldehydes & ketones) fatty acids Bio-based Oil CRACK
Upgrading biomass pyrolysis bio-oil to renewable fuels.
DOT National Transportation Integrated Search
2015-01-01
Fast pyrolysis is a process that can convert woody biomass to a crude bio-oil (pyrolysis oil). However, some of these compounds : contribute to bio-oil shelf life instability and difficulty in refining. Catalytic hydrodeoxygenation (HDO) of the bio-o...
BioC implementations in Go, Perl, Python and Ruby.
Liu, Wanli; Islamaj Doğan, Rezarta; Kwon, Dongseop; Marques, Hernani; Rinaldi, Fabio; Wilbur, W John; Comeau, Donald C
2014-01-01
As part of a communitywide effort for evaluating text mining and information extraction systems applied to the biomedical domain, BioC is focused on the goal of interoperability, currently a major barrier to wide-scale adoption of text mining tools. BioC is a simple XML format, specified by DTD, for exchanging data for biomedical natural language processing. With initial implementations in C++ and Java, BioC provides libraries of code for reading and writing BioC text documents and annotations. We extend BioC to Perl, Python, Go and Ruby. We used SWIG to extend the C++ implementation for Perl and one Python implementation. A second Python implementation and the Ruby implementation use native data structures and libraries. BioC is also implemented in the Google language Go. BioC modules are functional in all of these languages, which can facilitate text mining tasks. BioC implementations are freely available through the BioC site: http://bioc.sourceforge.net. Database URL: http://bioc.sourceforge.net/ Published by Oxford University Press 2014. This work is written by US Government employees and is in the public domain in the US.
NASA Technical Reports Server (NTRS)
Lewis, Brian; Hanel, Robert; Bhattacharya, Sharmila; Ricco, Antonion J.; Agasid, Elwood; Reiss-Bubenheim, Debra; Straume, Tore; Parra, Macerena; Boone, Travis; Santa Maria, Sergio;
2015-01-01
We are designing and developing a "6U" (10 x 22 x 34 cm; 14 kg) nanosatellite as a secondary payload to fly aboard NASA's Space Launch System (SLS) Exploration Mission (EM) 1, scheduled for launch in late 2017. For the first time in over forty years, direct experimental data from biological studies beyond low Earth orbit (LEO) will be obtained during BioSentinel's 12- to 18- month mission. BioSentinel will measure the damage and repair of DNA in a biological organism and allow us to compare that to information from onboard physical radiation sensors. In order to understand the relative contributions of the space environment's two dominant biological perturbations, reduced gravity and ionizing radiation, results from deep space will be directly compared to data obtained in LEO (on ISS) and on Earth. These data points will be available for validation of existing biological radiation damage and repair models, and for extrapolation to humans, to assist in mitigating risks during future long-term exploration missions beyond LEO. The BioSentinel Payload occupies 4U of the spacecraft and will utilize the monocellular eukaryotic organism Saccharomyces cerevisiae (yeast) to report DNA double-strand-break (DSB) events that result from ambient space radiation. DSB repair exhibits striking conservation of repair proteins from yeast to humans. Yeast was selected because of 1) its similarity to cells in higher organisms, 2) the well-established history of strains engineered to measure DSB repair, 3) its spaceflight heritage, and 4) the wealth of available ground and flight reference data. The S. cerevisiae flight strain will include engineered genetic defects to prevent growth and division until a radiation-induced DSB activates the yeast's DNA repair mechanisms. The triggered culture growth and metabolic activity directly indicate a DSB and its successful repair. The yeast will be carried in the dry state within the 1-atm P/L container in 18 separate fluidics cards with each
NASA Astrophysics Data System (ADS)
Ahmadi, Roozbeh; Kalantari, Masoud; Packirisamy, Muthukumaran; Dargahi, Javad
2010-06-01
Currently, Minimally Invasive Surgery (MIS) performs through keyhole incisions using commercially available robotic surgery systems. One of the most famous examples of these robotic surgery systems is the da Vinci surgical system. In the current robotic surgery systems like the da Vinci, surgeons are faced with problems such as lack of tactile feedback during the surgery. Therefore, providing a real-time tactile feedback from interaction between surgical instruments and tissue can help the surgeons to perform MIS more reliably. The present paper proposes an optical tactile sensor to measure the contact force between the bio-tissue and the surgical instrument. A model is proposed for simulating the interaction between a flexible membrane and bio-tissue based on the finite element methods. The tissue is considered as a hyperelastic material with the material properties similar to the heart tissue. The flexible membrane is assumed as a thin layer of silicon which can be microfabricated using the technology of Micro Electro Mechanical Systems (MEMS). The simulation results are used to optimize the geometric design parameters of a proposed MEMS tactile sensor for use in robotic surgical systems to perform MIS.
A bio-inspired real-time capable artificial lateral line system for freestream flow measurements.
Abels, C; Qualtieri, A; De Vittorio, M; Megill, W M; Rizzi, F
2016-06-03
To enhance today's artificial flow sensing capabilities in aerial and underwater robotics, future robots could be equipped with a large number of miniaturized sensors distributed over the surface to provide high resolution measurement of the surrounding fluid flow. In this work we show a linear array of closely separated bio-inspired micro-electro-mechanical flow sensors whose sensing mechanism is based on a piezoresistive strain-gauge along a stress-driven cantilever beam, mimicking the biological superficial neuromasts found in the lateral line organ of fishes. Aiming to improve state-of-the-art flow sensing capability in autonomously flying and swimming robots, our artificial lateral line system was designed and developed to feature multi-parameter freestream flow measurements which provide information about (1) local flow velocities as measured by the signal amplitudes from the individual cantilevers as well as (2) propagation velocity, (3) linear forward/backward direction along the cantilever beam orientation and (4) periodicity of pulses or pulse trains determined by cross-correlating sensor signals. A real-time capable cross-correlation procedure was developed which makes it possible to extract freestream flow direction and velocity information from flow fluctuations. The computed flow velocities deviate from a commercial system by 0.09 m s(-1) at 0.5 m s(-1) and 0.15 m s(-1) at 1.0 m s(-1) flow velocity for a sampling rate of 240 Hz and a sensor distance of 38 mm. Although experiments were performed in air, the presented flow sensing system can be applied to underwater vehicles as well, once the sensors are embedded in a waterproof micro-electro-mechanical systems package.
[Bio-oil production from biomass pyrolysis in molten salt].
Ji, Dengxiang; Cai, Tengyue; Ai, Ning; Yu, Fengwen; Jiang, Hongtao; Ji, Jianbing
2011-03-01
In order to investigate the effects of pyrolysis conditions on bio-oil production from biomass in molten salt, experiments of biomass pyrolysis were carried out in a self-designed reactor in which the molten salt ZnCl2-KCl (with mole ratio 7/6) was selected as heat carrier, catalyst and dispersion agent. The effects of metal salt added into ZnCl2-KCl and biomass material on biomass pyrolysis were discussed, and the main compositions of bio-oil were determined by GC-MS. Metal salt added into molten salt could affect pyrolysis production yields remarkably. Lanthanon salt could enhance bio-oil yield and decrease water content in bio-oil, when mole fraction of 5.0% LaCl3 was added, bio-oil yield could reach up to 32.0%, and water content of bio-oil could reduce to 61.5%. The bio-oil and char yields were higher when rice straw was pyrolysed, while gas yield was higher when rice husk was used. Metal salts showed great selectivity on compositions of bio-oil. LiCl and FeCl2 promoted biomass to pyrolyse into smaller molecular weight compounds. CrCl3, CaCl2 and LaCl3 could restrain second pyrolysis of bio-oil. The research provided a scientific reference for production of bio-oil from biomass pyrolysis in molten salt.
Wang, Yanli; Bryant, Stephen H.; Cheng, Tiejun; Wang, Jiyao; Gindulyte, Asta; Shoemaker, Benjamin A.; Thiessen, Paul A.; He, Siqian; Zhang, Jian
2017-01-01
PubChem's BioAssay database (https://pubchem.ncbi.nlm.nih.gov) has served as a public repository for small-molecule and RNAi screening data since 2004 providing open access of its data content to the community. PubChem accepts data submission from worldwide researchers at academia, industry and government agencies. PubChem also collaborates with other chemical biology database stakeholders with data exchange. With over a decade's development effort, it becomes an important information resource supporting drug discovery and chemical biology research. To facilitate data discovery, PubChem is integrated with all other databases at NCBI. In this work, we provide an update for the PubChem BioAssay database describing several recent development including added sources of research data, redesigned BioAssay record page, new BioAssay classification browser and new features in the Upload system facilitating data sharing. PMID:27899599
Design of pyrolysis reactor for production of bio-oil and bio-char simultaneously
NASA Astrophysics Data System (ADS)
Aladin, Andi; Alwi, Ratna Surya; Syarif, Takdir
2017-05-01
The residues from the wood industry are the main contributors to biomass waste in Indonesia. The conventional pyrolysis process, which needs a large energy as well as to produce various toxic chemical to the environment. Therefore, a pyrolysis unit on the laboratory scale was designed that can be a good alternative to achieve zero-waste and low energy cost. In this paper attempts to discuss design and system of pyrolysis reactor to produce bio-oil and bio-char simultaneously.
Stinging plants: as future bio-weapon.
Gupta, Sanjay Mohan; Kumar, Kamal
2016-09-01
In the present opinion paper, we have been introducing for the first time the stinging plants and/or their biological toxins as novel bio-threat agents that may be used for the development of bio-weapons for self-defence purpose. The selected studied stinging plants are having dual role as nutraceutical and ethno-pharmacological uses apart from their less explored stinging property. However, future detailed work is required for identification and characterization of the precise stinging chemical components that will be used for the formulation of novel bio-warfare agents for self-defence purpose.
BioBlend.objects: metacomputing with Galaxy.
Leo, Simone; Pireddu, Luca; Cuccuru, Gianmauro; Lianas, Luca; Soranzo, Nicola; Afgan, Enis; Zanetti, Gianluigi
2014-10-01
BioBlend.objects is a new component of the BioBlend package, adding an object-oriented interface for the Galaxy REST-based application programming interface. It improves support for metacomputing on Galaxy entities by providing higher-level functionality and allowing users to more easily create programs to explore, query and create Galaxy datasets and workflows. BioBlend.objects is available online at https://github.com/afgane/bioblend. The new object-oriented API is implemented by the galaxy/objects subpackage. © The Author 2014. Published by Oxford University Press.
Bio-Optical Measurements at Ocean Boundaries in Support of SIMBIOS. Chapter 7
NASA Technical Reports Server (NTRS)
Chavez, Francisco P.; Strutton, Peter G.; Schlining, Brian M.
2001-01-01
The equatorial Pacific is a major component of global biogeochemical cycles, due to upwelling that occurs from the coast of South America to beyond 180 deg. This upwelling has significant implications for global CO2 fluxes, as well as primary and secondary production. In addition, this region of the world's oceans represents a large oceanic province over which validation data for Sea-Viewing Wide Field-of-view Sensor (SeaWiFS) are necessary. This project consists of a mooring program and supporting cruise-based measurements aimed at quantifying the spectrum of biological and chemical variability in the equatorial Pacific and obtaining validation data for SeaWiFS. The project has the following general objectives: (1) to understand the relationships between physical forcing, primary production, nutrient supply and the exchange of carbon dioxide between ocean and atmosphere in the equatorial Pacific; (2) to describe the biological and chemical responses to climate and ocean variability; (3) to describe the spatial, seasonal and inter-annual variability in near surface plant pigments, primary production, carbon dioxide and nutrient distributions; and (4) to obtain near real-time bio-optical measurements for validation of SeaWiFS and subsequent ocean color sensors.
A predictive model for biomimetic plate type broadband frequency sensor
NASA Astrophysics Data System (ADS)
Ahmed, Riaz U.; Banerjee, Sourav
2016-04-01
In this work, predictive model for a bio-inspired broadband frequency sensor is developed. Broadband frequency sensing is essential in many domains of science and technology. One great example of such sensor is human cochlea, where it senses a frequency band of 20 Hz to 20 KHz. Developing broadband sensor adopting the physics of human cochlea has found tremendous interest in recent years. Although few experimental studies have been reported, a true predictive model to design such sensors is missing. A predictive model is utmost necessary for accurate design of selective broadband sensors that are capable of sensing very selective band of frequencies. Hence, in this study, we proposed a novel predictive model for the cochlea-inspired broadband sensor, aiming to select the frequency band and model parameters predictively. Tapered plate geometry is considered mimicking the real shape of the basilar membrane in the human cochlea. The predictive model is intended to develop flexible enough that can be employed in a wide variety of scientific domains. To do that, the predictive model is developed in such a way that, it can not only handle homogeneous but also any functionally graded model parameters. Additionally, the predictive model is capable of managing various types of boundary conditions. It has been found that, using the homogeneous model parameters, it is possible to sense a specific frequency band from a specific portion (B) of the model length (L). It is also possible to alter the attributes of `B' using functionally graded model parameters, which confirms the predictive frequency selection ability of the developed model.
The BioGRID Interaction Database: 2011 update
Stark, Chris; Breitkreutz, Bobby-Joe; Chatr-aryamontri, Andrew; Boucher, Lorrie; Oughtred, Rose; Livstone, Michael S.; Nixon, Julie; Van Auken, Kimberly; Wang, Xiaodong; Shi, Xiaoqi; Reguly, Teresa; Rust, Jennifer M.; Winter, Andrew; Dolinski, Kara; Tyers, Mike
2011-01-01
The Biological General Repository for Interaction Datasets (BioGRID) is a public database that archives and disseminates genetic and protein interaction data from model organisms and humans (http://www.thebiogrid.org). BioGRID currently holds 347 966 interactions (170 162 genetic, 177 804 protein) curated from both high-throughput data sets and individual focused studies, as derived from over 23 000 publications in the primary literature. Complete coverage of the entire literature is maintained for budding yeast (Saccharomyces cerevisiae), fission yeast (Schizosaccharomyces pombe) and thale cress (Arabidopsis thaliana), and efforts to expand curation across multiple metazoan species are underway. The BioGRID houses 48 831 human protein interactions that have been curated from 10 247 publications. Current curation drives are focused on particular areas of biology to enable insights into conserved networks and pathways that are relevant to human health. The BioGRID 3.0 web interface contains new search and display features that enable rapid queries across multiple data types and sources. An automated Interaction Management System (IMS) is used to prioritize, coordinate and track curation across international sites and projects. BioGRID provides interaction data to several model organism databases, resources such as Entrez-Gene and other interaction meta-databases. The entire BioGRID 3.0 data collection may be downloaded in multiple file formats, including PSI MI XML. Source code for BioGRID 3.0 is freely available without any restrictions. PMID:21071413
NASA Astrophysics Data System (ADS)
Cardoso, Elizabeth Carvalho L.; Seixas, Marcus Vinicius S.; Wiebeck, Helio; Oliveira, René R.; Machado, Glauson Aparecido F.; Moura, Esperidiana A. B.
In Brazil, the food industry generates every year huge amounts of avian eggshell waste, an industrial byproduct containing 95% of calcium carbonate, and its disposal constitutes a serious environmental hazard. This study aims to the development of bio-foams from PBAT/PLA blends reinforced with bio-calcium carbonate from eggshells. Composites were obtained by melting extrusion process, blending PBAT/PLA (50/50) with 25% of bio-calcium carbonate, PBAT/PLA (50/45) with 25% of bio-calcium carbonate and 5 % of pre-irradiated PLA and PBAT/PLA (50/40) with 25% of bio-calcium carbonate and 10 % of pre-irradiated PLA. PLA was previously e-beam irradiated at 150kGy in air and used as compatibilizer agent. The composites were then extruded in a Rheomex 332p single special screw for foaming. Samples were submitted to Tensile and Compression tests, MFI, DSC, TGA, XRD and FEG/SEM, analyses.
Chiou, Jin-Chern; Hsu, Shun-Hsi; Huang, Yu-Chieh; Yeh, Guan-Ting; Liou, Wei-Ting; Kuei, Cheng-Kai
2017-01-01
This study presented a wireless smart contact lens system that was composed of a reconfigurable capacitive sensor interface circuitry and wirelessly powered radio-frequency identification (RFID) addressable system for sensor control and data communication. In order to improve compliance and reduce user discomfort, a capacitive sensor was embedded on a soft contact lens of 200 μm thickness using commercially available bio-compatible lens material and a standard manufacturing process. The results indicated that the reconfigurable sensor interface achieved sensitivity and baseline tuning up to 120 pF while consuming only 110 μW power. The range and sensitivity tuning of the readout circuitry ensured a reliable operation with respect to sensor fabrication variations and independent calibration of the sensor baseline for individuals. The on-chip voltage scaling allowed the further extension of the detection range and prevented the implementation of large on-chip elements. The on-lens system enabled the detection of capacitive variation caused by pressure changes in the range of 2.25 to 30 mmHg and hydration level variation from a distance of 1 cm using incident power from an RFID reader at 26.5 dBm. PMID:28067859
Mafrica, Stefano; Servel, Alain; Ruffier, Franck
2016-11-10
Here we present a novel bio-inspired optic flow (OF) sensor and its application to visual guidance and odometry on a low-cost car-like robot called BioCarBot. The minimalistic OF sensor was robust to high-dynamic-range lighting conditions and to various visual patterns encountered thanks to its M 2 APIX auto-adaptive pixels and the new cross-correlation OF algorithm implemented. The low-cost car-like robot estimated its velocity and steering angle, and therefore its position and orientation, via an extended Kalman filter (EKF) using only two downward-facing OF sensors and the Ackerman steering model. Indoor and outdoor experiments were carried out in which the robot was driven in the closed-loop mode based on the velocity and steering angle estimates. The experimental results obtained show that our novel OF sensor can deliver high-frequency measurements ([Formula: see text]) in a wide OF range (1.5-[Formula: see text]) and in a 7-decade high-dynamic light level range. The OF resolution was constant and could be adjusted as required (up to [Formula: see text]), and the OF precision obtained was relatively high (standard deviation of [Formula: see text] with an average OF of [Formula: see text], under the most demanding lighting conditions). An EKF-based algorithm gave the robot's position and orientation with a relatively high accuracy (maximum errors outdoors at a very low light level: [Formula: see text] and [Formula: see text] over about [Formula: see text] and [Formula: see text]) despite the low-resolution control systems of the steering servo and the DC motor, as well as a simplified model identification and calibration. Finally, the minimalistic OF-based odometry results were compared to those obtained using measurements based on an inertial measurement unit (IMU) and a motor's speed sensor.
Protein-specific localization of a rhodamine-based calcium-sensor in living cells.
Best, Marcel; Porth, Isabel; Hauke, Sebastian; Braun, Felix; Herten, Dirk-Peter; Wombacher, Richard
2016-06-28
A small synthetic calcium sensor that can be site-specifically coupled to proteins in living cells by utilizing the bio-orthogonal HaloTag labeling strategy is presented. We synthesized an iodo-derivatized BAPTA chelator with a tetramethyl rhodamine fluorophore that allows further modification by Sonogashira cross-coupling. The presented calcium sensitive dye shows a 200-fold increase in fluorescence upon calcium binding. The derivatization with an aliphatic linker bearing a terminal haloalkane-function by Sonogashira cross-coupling allows the localization of the calcium sensor to Halo fusion proteins which we successfully demonstrate in in vitro and in vivo experiments. The herein reported highly sensitive tetramethyl rhodamine based calcium indicator, which can be selectively localized to proteins, is a powerful tool to determine changes in calcium levels inside living cells with spatiotemporal resolution.
Temperature insensitive refractive index sensor based on concatenated long period fiber gratings
NASA Astrophysics Data System (ADS)
Tripathi, Saurabh M.; Bock, Wojtek J.; Mikulic, Predrag
2013-10-01
We propose and demonstrate a temperature immune biosensor based on two concatenated LPGs incorporating a suitable inter-grating-space (IGS). Compensating the thermal induced phase changes in the grating region by use of an appropriate length of the IGS the temperature insensitivity has been achieved. Using standard telecommunication grade single-mode fibers we show that a length ratio of ~8.2 is sufficient to realize the proposed temperature insensitivity. The resulting sensor shows a refractive index sensitivity of 423.28 nm/RIU displaying the capability of detecting an index variation of 2.36 × 10-6 RIU in the bio-samples. The sensor can also be applied as a temperature insensitive WMD channel isolation filter in the optical communication systems, removing the necessity of any external thermal insulation packaging.
A Graphene-Based Resistive Pressure Sensor with Record-High Sensitivity in a Wide Pressure Range
Tian, He; Shu, Yi; Wang, Xue-Feng; Mohammad, Mohammad Ali; Bie, Zhi; Xie, Qian-Yi; Li, Cheng; Mi, Wen-Tian; Yang, Yi; Ren, Tian-Ling
2015-01-01
Pressure sensors are a key component in electronic skin (e-skin) sensing systems. Most reported resistive pressure sensors have a high sensitivity at low pressures (<5 kPa) to enable ultra-sensitive detection. However, the sensitivity drops significantly at high pressures (>5 kPa), which is inadequate for practical applications. For example, actions like a gentle touch and object manipulation have pressures below 10 kPa, and 10–100 kPa, respectively. Maintaining a high sensitivity in a wide pressure range is in great demand. Here, a flexible, wide range and ultra-sensitive resistive pressure sensor with a foam-like structure based on laser-scribed graphene (LSG) is demonstrated. Benefitting from the large spacing between graphene layers and the unique v-shaped microstructure of the LSG, the sensitivity of the pressure sensor is as high as 0.96 kPa−1 in a wide pressure range (0 ~ 50 kPa). Considering both sensitivity and pressure sensing range, the pressure sensor developed in this work is the best among all reported pressure sensors to date. A model of the LSG pressure sensor is also established, which agrees well with the experimental results. This work indicates that laser scribed flexible graphene pressure sensors could be widely used for artificial e-skin, medical-sensing, bio-sensing and many other areas. PMID:25721159
Optical and electrical interfacing technologies for living cell bio-chips.
Shacham-Diamand, Y; Belkin, S; Rishpon, J; Elad, T; Melamed, S; Biran, A; Yagur-Kroll, S; Almog, R; Daniel, R; Ben-Yoav, H; Rabner, A; Vernick, S; Elman, N; Popovtzer, R
2010-06-01
Whole-cell bio-chips for functional sensing integrate living cells on miniaturized platforms made by micro-system-technologies (MST). The cells are integrated, deposited or immersed in a media which is in contact with the chip. The cells behavior is monitored via electrical, electrochemical or optical methods. In this paper we describe such whole-cell biochips where the signal is generated due to the genetic response of the cells. The solid-state platform hosts the biological component, i.e. the living cells, and integrates all the required micro-system technologies, i.e. the micro-electronics, micro-electro optics, micro-electro or magneto mechanics and micro-fluidics. The genetic response of the cells expresses proteins that generate: a. light by photo-luminescence or bioluminescence, b. electrochemical signal by interaction with a substrate, or c. change in the cell impedance. The cell response is detected by a front end unit that converts it to current or voltage amplifies and filters it. The resultant signal is analyzed and stored for further processing. In this paper we describe three examples of whole-cell bio chips, photo-luminescent, bioluminescent and electrochemical, which are based on the genetic response of genetically modified E. coli microbes integrated on a micro-fluidics MEMS platform. We describe the chip outline as well as the basic modeling scheme of such sensors. We discuss the highlights and problems of such system, from the point of view of micro-system-technology.
Acidity of biomass fast pyrolysis bio-oils
DOE Office of Scientific and Technical Information (OSTI.GOV)
Oasmaa, Anja; Elliott, Douglas C.; Korhonen, Jaana
2010-12-17
The use of the TAN method for measuring the acidity of biomass fast pyrolysis bio-oil was evaluated. Suggestions for carrying out the analysis have been made. The TAN method by ASTM D664 or D3339 can be used for measuring the acidity of fast pyrolysis bio-oils and their hydrotreating products. The main difference between the methods is that ASTM D664 is specified for higher TAN values than ASTM D3339. Special focus should be placed on the interpretation of the TAN curves because they differ significantly from those of mineral oils. The curve for bio-oils is so gentle that the automatic detectionmore » may not observe the end point properly and derivatization should be used. The acidity of fast pyrolysis bio-oils is mainly derived (60-70%) from volatile acids. Other groups of compounds in fast pyrolysis bio-oils that influence acidity include phenolics, fatty and resin acids, and hydroxy acids.« less
Measuring average angular velocity with a smartphone magnetic field sensor
NASA Astrophysics Data System (ADS)
Pili, Unofre; Violanda, Renante
2018-02-01
The angular velocity of a spinning object is, by standard, measured using a device called a tachometer. However, by directly using it in a classroom setting, the activity is likely to appear as less instructive and less engaging. Indeed, some alternative classroom-suitable methods for measuring angular velocity have been presented. In this paper, we present a further alternative that is smartphone-based, making use of the real-time magnetic field (simply called B-field in what follows) data gathering capability of the B-field sensor of the smartphone device as the timer for measuring average rotational period and average angular velocity. The in-built B-field sensor in smartphones has already found a number of uses in undergraduate experimental physics. For instance, in elementary electrodynamics, it has been used to explore the well-known Bio-Savart law and in a measurement of the permeability of air.
STABILIZATION OF BIO-OIL TO ENABLE ITS HYDROTREATING TO PRODUCE BIOFUELS
DOE Office of Scientific and Technical Information (OSTI.GOV)
Wang, Huamin
Fast pyrolysis is considered to be the simplest and most cost-effective approach to produce liquid oil (bio-oil) from biomass. Bio-oil is not suitable to substitute for petroleum as high-quality fuels and significant upgrading such as hydrotreating is required to remove oxygen, add hydrogen, and rearrange carbon backbone of bio-oil. However, the grand challenge in bio-oil hydrotreating technology is bio-oil instability, which limits the lifetime of catalyst and operation. To enable a sustainable and economically viable process for bio-oil hydrotreating, it is vital to develop effective technologies for stabilizing bio-oils. This chapter will be devoted to bio-oil stabilization. The current understatingmore » of the major cause of bio-oil instability, condensation of reactive species such as sugar, aldehydes, ketones, and phenolics, is elucidated. The reported physical and chemical methods for bio-oil stabilization are summarized in detail, with a specific focus on bio-oil catalytic hydrogenation for stabilization. The impact of stabilization on bio-oil hydrotreating is discussed as well.« less
Beclometasone oral--DOR BioPharma.
2007-01-01
orBec is an oral enteric-coated tablet formulation of the corticosteroid beclometasone, which has been developed by Enteron Pharmaceuticals, a subsidiary of Corporate Technology Development (now DOR BioPharma). orBec is being developed for the treatment of gastrointestinal graft-versus-host disease (GVHD) and an NDA has been filed in the US. DOR BioPharma has also filed an MAA in Europe for the same indication.orBec is designed to reduce the need for systemic immunosuppressive drugs, thereby improving the outcome of bone marrow and stem cell transplantation.DOR BioPharma may seek a marketing partner in the US and elsewhere for orBec in GVHD and will seek a partner for other potential indications of the drug.In December 2001, Corporate Technology Development was acquired by Endorex Corporation (now DOR BioPharma). In October 1998, Enteron Pharmaceuticals (DOR BioPharma) entered into an exclusive, worldwide, royalty bearing license agreement with George B. McDonald, MD, including the right to grant sublicenses, for the rights to the intellectual property and know-how relating to orBec. In January 2007, DOR BioPharma received $US3 million under a non-binding letter of intent from Sigma-Tau Pharmaceuticals. The agreement grants Sigma-Tau an exclusive right to negotiate terms and conditions for a possible business transaction or strategic alliance regarding orBec and potentially other DOR pipeline compounds until 1 March 2007. Under the terms of the agreement, Sigma-Tau purchased $US1 million of DOR's common stock, with an additional $US2 million paid in cash. If no agreement is reached by 1 March 2007, DOR will return the $US2 million to Sigma-Tau within 60 days. DOR BioPharma received an unsolicited proposal from Cell Therapeutics, Inc. to acquire DOR BioPharma in January 2007. Because of the non-binding agreement already signed with Sigma-Tau, DOR BioPharma's board of directors cannot consider Cell Therapeutics' merger proposal at this time. orBec has been filed for
The BioGRID interaction database: 2017 update
Chatr-aryamontri, Andrew; Oughtred, Rose; Boucher, Lorrie; Rust, Jennifer; Chang, Christie; Kolas, Nadine K.; O'Donnell, Lara; Oster, Sara; Theesfeld, Chandra; Sellam, Adnane; Stark, Chris; Breitkreutz, Bobby-Joe; Dolinski, Kara; Tyers, Mike
2017-01-01
The Biological General Repository for Interaction Datasets (BioGRID: https://thebiogrid.org) is an open access database dedicated to the annotation and archival of protein, genetic and chemical interactions for all major model organism species and humans. As of September 2016 (build 3.4.140), the BioGRID contains 1 072 173 genetic and protein interactions, and 38 559 post-translational modifications, as manually annotated from 48 114 publications. This dataset represents interaction records for 66 model organisms and represents a 30% increase compared to the previous 2015 BioGRID update. BioGRID curates the biomedical literature for major model organism species, including humans, with a recent emphasis on central biological processes and specific human diseases. To facilitate network-based approaches to drug discovery, BioGRID now incorporates 27 501 chemical–protein interactions for human drug targets, as drawn from the DrugBank database. A new dynamic interaction network viewer allows the easy navigation and filtering of all genetic and protein interaction data, as well as for bioactive compounds and their established targets. BioGRID data are directly downloadable without restriction in a variety of standardized formats and are freely distributed through partner model organism databases and meta-databases. PMID:27980099
Direct Bio-printing with Heterogeneous Topology Design.
Ahsan, Amm Nazmul; Xie, Ruinan; Khoda, Bashir
2017-01-01
Bio-additive manufacturing is a promising tool to fabricate porous scaffold structures for expediting the tissue regeneration processes. Unlike the most traditional bulk material objects, the microstructures of tissue and organs are mostly highly anisotropic, heterogeneous, and porous in nature. However, modelling the internal heterogeneity of tissues/organs structures in the traditional CAD environment is difficult and oftentimes inaccurate. Besides, the de facto STL conversion of bio-models introduces loss of information and piles up more errors in each subsequent step (build orientation, slicing, tool-path planning) of the bio-printing process plan. We are proposing a topology based scaffold design methodology to accurately represent the heterogeneous internal architecture of tissues/organs. An image analysis technique is used that digitizes the topology information contained in medical images of tissues/organs. A weighted topology reconstruction algorithm is implemented to represent the heterogeneity with parametric functions. The parametric functions are then used to map the spatial material distribution. The generated information is directly transferred to the 3D bio-printer and heterogeneous porous tissue scaffold structure is manufactured without STL file. The proposed methodology is implemented to verify the effectiveness of the approach and the designed example structure is bio-fabricated with a deposition based bio-additive manufacturing system.
Confocal epifluorescence sensor with an arc-shaped aperture for slide-based PCR quantification.
Weng, Jui-Hong; Chen, Lin-Chi
2018-02-15
The increasing needs of point-of-care diagnostics, quarantine of epidemic pathogens, and prevention of terrorism's bio-attacks have promised the future of portable real-time quantitative polymerase chain reaction (qPCR) sensors. This work aims at developing a highly sensitive and low-cost light emitting diode (LED)-based epifluorescence sensor module for qPCR sensor development and relevant bioassay applications. Inspired by the light stop design and dark-field detection of microscopes, this paper first reports a compact confocal LED epifluorescence sensor using a light stop with an arc-shaped aperture for enhancing the flexibility of quick DNA and PCR detection. The sensor features the advantages of the dichroic mirror-free and confocal (shared-focus) characteristics, which benefits size reduction and minimal optics used. It also allows extension to integrate with in situ real-time PCR thermal cycling since the sample slide is placed apart from the epi-sensing module. The epifluorescence sensor can detect as low as sub-ng/μL standard DNA and 10 1 copies of Salmonella typhimurium InvA gene sequences (cloned in E. coli and after 30-cycle PCR) with SYBR ® Green I from non-purified culture samples, having highly sensitive and specific signal responses comparable with that of a commercial qPCR instrument. Copyright © 2017 Elsevier B.V. All rights reserved.
Boron brings big benefits to bio-based blends
USDA-ARS?s Scientific Manuscript database
The solution to the problems with bio-based lubrication can be approached by a combination of blending and additive strategies. However, many additives do not show efficacy when used in bio-based lubricants. Additive addition also lowers the bio-based content of the blend, which in turn limits the a...
Spider Silk: Mother Nature's Bio-Superlens
NASA Astrophysics Data System (ADS)
Monks, James N.; Yan, Bing; Hawkins, Nicholas; Vollrath, Fritz; Wang, Zengbo
2016-09-01
This paper demonstrates a possible new microfiber bio near field lens that uses minor ampullate spider silk,spun from the Nephila edulis spider, to create a real time image of a surface using near field optical techniques. The microfiber bio lens is the world's first natural superlens created by exploring biological materials. The resolution of the surface image overcomes the diffraction limit, with the ability to resolve patterns at 100 nm under a standard white light source in reflection mode. This resolution offers further developments in superlens technology and paves the way for new bio optics.
Branding the bio/biomedical engineering degree.
Voigt, Herbert F
2011-01-01
The future challenges to medical and biological engineering, sometimes referred to as biomedical engineering or simply bioengineering, are many. Some of these are identifiable now and others will emerge from time to time as new technologies are introduced and harnessed. There is a fundamental issue regarding "Branding the bio/biomedical engineering degree" that requires a common understanding of what is meant by a B.S. degree in Biomedical Engineering, Bioengineering, or Biological Engineering. In this paper we address some of the issues involved in branding the Bio/Biomedical Engineering degree, with the aim of clarifying the Bio/Biomedical Engineering brand.
Quercetin as natural stabilizing agent for bio-polymer
DOE Office of Scientific and Technical Information (OSTI.GOV)
Morici, Elisabetta; Arrigo, Rossella; Dintcheva, Nadka Tzankova
The introduction of antioxidants in polymers is the main way to prevent or delay the degradation process. In particular natural antioxidants receive attention in the food industry also because of their presumed safety. In this work bio-polymers, i.e. a commercial starch-based polymer (Mater-Bi®) and a bio-polyester (PLA), and a bio-polyether (PEO) were additivated with quercetin, a natural flavonoid antioxidants, in order to formulate bio-based films for ecosustainable packaging and outdoor applications. The photo-oxidation behavior of unstabilized and quercetin stabilized films was analyzed and compared with the behavior of films additivated with a commercial synthetic light stabilizer. The quercetin is ablemore » to slow down the photo-degradation rate of all bio-polymeric films investigated in similar way to the synthetic stabilizer.« less
Quercetin as natural stabilizing agent for bio-polymer
NASA Astrophysics Data System (ADS)
Morici, Elisabetta; Arrigo, Rossella; Dintcheva, Nadka Tzankova
2014-05-01
The introduction of antioxidants in polymers is the main way to prevent or delay the degradation process. In particular natural antioxidants receive attention in the food industry also because of their presumed safety. In this work bio-polymers, i.e. a commercial starch-based polymer (Mater-Bi®) and a bio-polyester (PLA), and a bio-polyether (PEO) were additivated with quercetin, a natural flavonoid antioxidants, in order to formulate bio-based films for ecosustainable packaging and outdoor applications. The photo-oxidation behavior of unstabilized and quercetin stabilized films was analyzed and compared with the behavior of films additivated with a commercial synthetic light stabilizer. The quercetin is able to slow down the photo-degradation rate of all bio-polymeric films investigated in similar way to the synthetic stabilizer.
Wang, Li-Chun; Su, Tseng-Hsiung; Ho, Cheng-Long; Yang, Shang-Ren; Chiu, Shih-Wen; Kuo, Han-Wen; Tang, Kea-Tiong
2015-01-01
In this paper, we propose a bio-inspired, two-layer, multiple-walled carbon nanotube (MWCNT)-polypeptide composite sensing device. The MWCNT serves as a responsive and conductive layer, and the nonselective polypeptide (40 mer) coating the top of the MWCNT acts as a filter into which small molecular gases pass. Instead of using selective peptides to sense specific odorants, we propose using nonselective, peptide-based sensors to monitor various types of volatile organic compounds. In this study, depending on gas interaction and molecular sizes, the randomly selected polypeptide enabled the recognition of certain polar volatile chemical vapors, such as amines, and the improved discernment of low-concentration gases. The results of our investigation demonstrated that the polypeptide-coated sensors can detect ammonia at a level of several hundred ppm and barely responded to triethylamine. PMID:25751078
A Survey of Business Trends at BioOne Publishing Partners and its Implications for BioOne
ERIC Educational Resources Information Center
Carpenter, Todd A.; Joseph, Heather; Waltham, Mary
2004-01-01
This paper describes a survey of BioOne participating publishers that was conducted during the fall of 2003. In that survey, BioOne collected data from 18 not-for-profit publishers on circulation levels, scholarly output in terms of pages and articles produced, revenues, and expenditures. From eight of the publishers, complete profit, loss, and…
Multimodal Bio-Inspired Tactile Sensing Module for Surface Characterization †
Alves de Oliveira, Thiago Eustaquio; Cretu, Ana-Maria; Petriu, Emil M.
2017-01-01
Robots are expected to recognize the properties of objects in order to handle them safely and efficiently in a variety of applications, such as health and elder care, manufacturing, or high-risk environments. This paper explores the issue of surface characterization by monitoring the signals acquired by a novel bio-inspired tactile probe in contact with ridged surfaces. The tactile module comprises a nine Degree of Freedom Microelectromechanical Magnetic, Angular Rate, and Gravity system (9-DOF MEMS MARG) and a deep MEMS pressure sensor embedded in a compliant structure that mimics the function and the organization of mechanoreceptors in human skin as well as the hardness of the human skin. When the modules tip slides over a surface, the MARG unit vibrates and the deep pressure sensor captures the overall normal force exerted. The module is evaluated in two experiments. The first experiment compares the frequency content of the data collected in two setups: one when the module is mounted over a linear motion carriage that slides four grating patterns at constant velocities; the second when the module is carried by a robotic finger in contact with the same grating patterns while performing a sliding motion, similar to the exploratory motion employed by humans to detect object roughness. As expected, in the linear setup, the magnitude spectrum of the sensors’ output shows that the module can detect the applied stimuli with frequencies ranging from 3.66 Hz to 11.54 Hz with an overall maximum error of ±0.1 Hz. The second experiment shows how localized features extracted from the data collected by the robotic finger setup over seven synthetic shapes can be used to classify them. The classification method consists on applying multiscale principal components analysis prior to the classification with a multilayer neural network. Achieved accuracies from 85.1% to 98.9% for the various sensor types demonstrate the usefulness of traditional MEMS as tactile sensors embedded
Bio-microfluidics: biomaterials and biomimetic designs.
Domachuk, Peter; Tsioris, Konstantinos; Omenetto, Fiorenzo G; Kaplan, David L
2010-01-12
Bio-microfluidics applies biomaterials and biologically inspired structural designs (biomimetics) to microfluidic devices. Microfluidics, the techniques for constraining fluids on the micrometer and sub-micrometer scale, offer applications ranging from lab-on-a-chip to optofluidics. Despite this wealth of applications, the design of typical microfluidic devices imparts relatively simple, laminar behavior on fluids and is realized using materials and techniques from silicon planar fabrication. On the other hand, highly complex microfluidic behavior is commonplace in nature, where fluids with nonlinear rheology flow through chaotic vasculature composed from a range of biopolymers. In this Review, the current state of bio-microfluidic materials, designs and applications are examined. Biopolymers enable bio-microfluidic devices with versatile functionalization chemistries, flexibility in fabrication, and biocompatibility in vitro and in vivo. Polymeric materials such as alginate, collagen, chitosan, and silk are being explored as bulk and film materials for bio-microfluidics. Hydrogels offer options for mechanically functional devices for microfluidic systems such as self-regulating valves, microlens arrays and drug release systems, vital for integrated bio-microfluidic devices. These devices including growth factor gradients to study cell responses, blood analysis, biomimetic capillary designs, and blood vessel tissue culture systems, as some recent examples of inroads in the field that should lead the way in a new generation of microfluidic devices for bio-related needs and applications. Perhaps one of the most intriguing directions for the future will be fully implantable microfluidic devices that will also integrate with existing vasculature and slowly degrade to fully recapitulate native tissue structure and function, yet serve critical interim functions, such as tissue maintenance, drug release, mechanical support, and cell delivery.
Bio and health informatics meets cloud : BioVLab as an example.
Chae, Heejoon; Jung, Inuk; Lee, Hyungro; Marru, Suresh; Lee, Seong-Whan; Kim, Sun
2013-01-01
The exponential increase of genomic data brought by the advent of the next or the third generation sequencing (NGS) technologies and the dramatic drop in sequencing cost have driven biological and medical sciences to data-driven sciences. This revolutionary paradigm shift comes with challenges in terms of data transfer, storage, computation, and analysis of big bio/medical data. Cloud computing is a service model sharing a pool of configurable resources, which is a suitable workbench to address these challenges. From the medical or biological perspective, providing computing power and storage is the most attractive feature of cloud computing in handling the ever increasing biological data. As data increases in size, many research organizations start to experience the lack of computing power, which becomes a major hurdle in achieving research goals. In this paper, we review the features of publically available bio and health cloud systems in terms of graphical user interface, external data integration, security and extensibility of features. We then discuss about issues and limitations of current cloud systems and conclude with suggestion of a biological cloud environment concept, which can be defined as a total workbench environment assembling computational tools and databases for analyzing bio/medical big data in particular application domains.
Study on a Luminol-based Electrochemiluminescent Sensor for Label-Free DNA Sensing
Chu, Hai-Hong; Yan, Ji-Lin; Tu, Yi-Feng
2010-01-01
Automatic, inexpensive, simple and sensitive methods for DNA sensing and quantification are highly desirable for biomedical research. The rapid development of both the fundamentals and applications of electrochemiluminescence (ECL) over the past years has demonstrated its potential for analytical and bio-analytical chemistry. This paper reports the quenching effect of DNA on the ECL of luminol and the further development of a DNA sensing device. With the pre-functionalization by a composite of carbon nano-tubes (CNTs) and Au nanoparticles (AuNPs), the sensor provides a novel and valuable label-free approach for DNA sensing. Here the ECL intensity was remarkably decreased when more than 1.0 × 10−12 molar of DNA were adsorbed on the sensor. Linearity of the DNA amount with the reciprocal of ECL intensity was observed. A saturated sensor caused a 92.8% quenching effect. The research also proposes the mechanism for the quenching effect which could be attributed to the interaction between luminol and DNA and the elimination of reactive oxygen species (ROSs) by DNA. PMID:22163421
Gating capacitive field-effect sensors by the charge of nanoparticle/molecule hybrids.
Poghossian, Arshak; Bäcker, Matthias; Mayer, Dirk; Schöning, Michael J
2015-01-21
The semiconductor field-effect platform is a powerful tool for chemical and biological sensing with direct electrical readout. In this work, the field-effect capacitive electrolyte-insulator-semiconductor (EIS) structure - the simplest field-effect (bio-)chemical sensor - modified with citrate-capped gold nanoparticles (AuNPs) has been applied for a label-free electrostatic detection of charged molecules by their intrinsic molecular charge. The EIS sensor detects the charge changes in AuNP/molecule inorganic/organic hybrids induced by the molecular adsorption or binding events. The feasibility of the proposed detection scheme has been exemplarily demonstrated by realizing capacitive EIS sensors consisting of an Al-p-Si-SiO2-silane-AuNP structure for the label-free detection of positively charged cytochrome c and poly-d-lysine molecules as well as for monitoring the layer-by-layer formation of polyelectrolyte multilayers of poly(allylamine hydrochloride)/poly(sodium 4-styrene sulfonate), representing typical model examples of detecting small proteins and macromolecules and the consecutive adsorption of positively/negatively charged polyelectrolytes, respectively. For comparison, EIS sensors without AuNPs have been investigated, too. The adsorption of molecules on the surface of AuNPs has been verified via the X-ray photoelectron spectroscopy method. In addition, a theoretical model of the functioning of the capacitive field-effect EIS sensor functionalized with AuNP/charged-molecule hybrids has been discussed.
Bio-oil transport by pipeline: a techno-economic assessment.
Pootakham, Thanyakarn; Kumar, Amit
2010-09-01
Bio-oil, produced by fast pyrolysis of biomass, has high energy density compared to 'as received' biomass. The study assesses and compares the cost of transportation ($/liter of bio-oil) of bio-oil by pipeline and truck. The fixed and variable cost components of transportation of bio-oil at a pipeline capacity of 560 m(3)/day and to a distance of 100 km are 0.0423$/m(3) and 0.1201$/m(3)/km, respectively. Pipeline transportation of bio-oil costs less than transportation by liquid tank truck (load capacity 30 m(3)) and super B-train trailer (load capacity 60 m(3)) above pipeline capacities of 1000 and 1700 m(3)/day, respectively. When transportation distance is greater than 100 km, bio-oil must be heated at booster stations. When transporting bio-oil by pipeline to a distance of 400 km, minimum pipeline capacities of 1150 and 2000 m(3)/day are required to compete economically with liquid tank trucks and super B-train tank trailers, respectively. Copyright 2010 Elsevier Ltd. All rights reserved.
Lowe, Benjamin M; Sun, Kai; Zeimpekis, Ioannis; Skylaris, Chris-Kriton; Green, Nicolas G
2017-11-06
Field-Effect Transistor sensors (FET-sensors) have been receiving increasing attention for biomolecular sensing over the last two decades due to their potential for ultra-high sensitivity sensing, label-free operation, cost reduction and miniaturisation. Whilst the commercial application of FET-sensors in pH sensing has been realised, their commercial application in biomolecular sensing (termed BioFETs) is hindered by poor understanding of how to optimise device design for highly reproducible operation and high sensitivity. In part, these problems stem from the highly interdisciplinary nature of the problems encountered in this field, in which knowledge of biomolecular-binding kinetics, surface chemistry, electrical double layer physics and electrical engineering is required. In this work, a quantitative analysis and critical review has been performed comparing literature FET-sensor data for pH-sensing with data for sensing of biomolecular streptavidin binding to surface-bound biotin systems. The aim is to provide the first systematic, quantitative comparison of BioFET results for a single biomolecular analyte, specifically streptavidin, which is the most commonly used model protein in biosensing experiments, and often used as an initial proof-of-concept for new biosensor designs. This novel quantitative and comparative analysis of the surface potential behaviour of a range of devices demonstrated a strong contrast between the trends observed in pH-sensing and those in biomolecule-sensing. Potential explanations are discussed in detail and surface-chemistry optimisation is shown to be a vital component in sensitivity-enhancement. Factors which can influence the response, yet which have not always been fully appreciated, are explored and practical suggestions are provided on how to improve experimental design.
BioMEMS for mitochondria medicine
NASA Astrophysics Data System (ADS)
Padmaraj, Divya
A BioMEMS device to study cell-mitochondrial physiological functionalities was developed. The pathogenesis of many diseases including obesity, diabetes and heart failure as well as aging has been linked to functional defects of mitochondria. The synthesis of Adenosine Tri Phosphate (ATP) is determined by the electrical potential across the inner mitochondrial membrane and by the pH difference due to proton flux across it. Therefore, electrical characterization by E-fields with complementary chemical testing was used here. The BioMEMS device was fabricated as an SU-8 based microfluidic system with gold electrodes on SiO2/Si wafers for electromagnetic interrogation. Ion Sensitive Field Effect Transistors (ISFETs) were incorporated for proton studies important in the electron transport chain, together with monitoring Na+, K+ and Ca++ ions for ion channel studies. ISFETs are chemically sensitive Metal Oxide Semiconductor Field Effect Transistor (MOSFET) devices and their threshold voltage is directly proportional to the electrolytic H+ ion variation. These ISFETs (sensitivity ˜55 mV/pH for H+) were further realized as specific ion sensitive Chemical Field Effect Transistors (CHEMFETs) by depositing a specific ion sensitive membrane on the gate. Electrodes for dielectric spectroscopy studies of mitochondria were designed as 2- and 4-probe structures for optimized operation over a wide frequency range. In addition, to limit polarization effects, a 4-electrode set-up with unique meshed pickup electrodes (7.5x7.5 mum2 loops with 4 mum wires) was fabricated. Sensitivity of impedance spectroscopy to membrane potential changes was confirmed by studying the influence of uncouplers and glucose on mitochondria. An electrical model was developed for the mitochondrial sample, and its frequency response correlated with impedance spectroscopy experiments of sarcolemmal mitochondria. Using the mesh electrode structure, we obtained a reduction of 83.28% in impedance at 200 Hz. COMSOL
Bio-objects’ political capacity: a research agenda
Maeseele, Pieter; Hendrickx, Kim; Pavone, Vincenzo; Van Hoyweghen, Ine
2013-01-01
This article explores the merits of foregrounding the dichotomy of politicization vs de-politicization for our understanding of bio-objects in order to study their production, circulation, and governance in European societies. By asking how bio-objects are configured in science, policy, public, and media discourses and practices, we focus on the role of socio-technical configurations in generating political relations. The bio-object thereby serves as an entry point to approach and conceptualize “the political” in an innovative way. PMID:23630150
Dowson, Duncan
2012-01-01
It is now forty six years since the separate topics of friction, lubrication, wear and bearing design were integrated under the title 'Tribology' [Department of Education and Science, Lubrication (Tribology) Education and Research. A Report on the Present Position and Industry's Needs, HMSO, London, 1966]. Significant developments have been reported in many established and new aspects of tribology during this period. The subject has contributed to improved performance of much familiar equipment, such as reciprocating engines, where there have been vast improvements in engine reliability and efficiency. Nano-tribology has been central to remarkable advances in information processing and digital equipment. Shortly after widespread introduction of the term tribology, integration with biology and medicine prompted rapid and extensive interest in the fascinating sub-field now known as Bio-tribology [D. Dowson and V. Wright, Bio-tribology, in The Rheology of Lubricants, ed. T. C. Davenport, Applied Science Publishers, Barking, 1973, pp. 81-88]. An outline will be given of some of the developments in the latter field.
Aludden, H C; Mordenfeld, A; Hallman, M; Dahlin, C; Jensen, T
2017-08-01
The objective of this systematic review was to test the hypothesis of no difference in implant treatment outcomes when using Bio-Oss alone or Bio-Oss mixed with particulate autogenous bone grafts for lateral ridge augmentation. A search of the MEDLINE, Cochrane Library, and Embase databases in combination with a hand-search of relevant journals was conducted. Human studies published in English from 1 January 1990 to 1 May 2016 were included. The search provided 337 titles and six studies fulfilled the inclusion criteria. Considerable variation prevented a meta-analysis from being performed. The two treatment modalities have never been compared within the same study. Non-comparative studies demonstrated a 3-year implant survival of 96% with 50% Bio-Oss mixed with 50% autogenous bone graft. Moreover, Bio-Oss alone or Bio-Oss mixed with autogenous bone graft seems to increase the amount of newly formed bone as well as the width of the alveolar process. Within the limitations of this systematic review, lateral ridge augmentation with Bio-Oss alone or in combination with autogenous bone graft seems to induce newly formed bone and increase the width of the alveolar process, with high short-term implant survival. However, long-term studies comparing the two treatment modalities are needed before final conclusions can be drawn. Copyright © 2017 International Association of Oral and Maxillofacial Surgeons. Published by Elsevier Ltd. All rights reserved.
Computing with motile bio-agents
NASA Astrophysics Data System (ADS)
Nicolau, Dan V., Jr.; Burrage, Kevin; Nicolau, Dan V.
2007-12-01
We describe a model of computation of the parallel type, which we call 'computing with bio-agents', based on the concept that motions of biological objects such as bacteria or protein molecular motors in confined spaces can be regarded as computations. We begin with the observation that the geometric nature of the physical structures in which model biological objects move modulates the motions of the latter. Consequently, by changing the geometry, one can control the characteristic trajectories of the objects; on the basis of this, we argue that such systems are computing devices. We investigate the computing power of mobile bio-agent systems and show that they are computationally universal in the sense that they are capable of computing any Boolean function in parallel. We argue also that using appropriate conditions, bio-agent systems can solve NP-complete problems in probabilistic polynomial time.
Tang, Hao L; Xie, Yuefeng F; Chen, Yen-Chih
2012-11-01
This research investigated the application of Bio-Amp, a commercial bio-additive for the treatment of fat, oil, and grease (FOG) in a grease trap, and evaluated potential impacts of treated effluent on downstream collection system and treatment processes. Results show that after Bio-Amp treatment, FOG deposit formation was reduced by 40%, implicating a potential reduction of sewer line blockages. Chemical oxygen demand (COD), total nitrogen (TN), total phosphorus (TP) and total fatty acids were reduced by 39%, 33%, 56%, and 59%, respectively, which represents an overall loading reduction of 9% COD, 5% TN and 40% TP received by the treatment plant from all the dining halls. On the other hand, readily biodegradable COD fractions significantly increased, which implies a potential improvement on Bio-P removal. Overall, the results showed that application of Bio-Amp in grease trap provides potential reduction of sewer line blockages, and can also alleviate downstream treatment burden. Copyright © 2012 Elsevier Ltd. All rights reserved.
Israel Marine Bio-geographic Database (ISRAMAR-BIO)
NASA Astrophysics Data System (ADS)
Greengrass, Eyal; Krivenko, Yevgeniya; Ozer, Tal; Ben Yosef, Dafna; Tom, Moshe; Gertman, Isaac
2015-04-01
The knowledge of the space/time variations of species is the basis for any ecological investigations. While historical observations containing integral concentrations of biological parameters (chlorophyll, abundance, biomass…) are organized partly in ISRAMAR Cast Database, the taxon-specific data collected in Israel has not been sufficiently organized. This has been hindered by the lack of standards, variability of methods and complexity of biological data formalization. The ISRAMAR-BIO DB was developed to store various types of historical and future available information related to marine species observations and related metadata. Currently the DB allows to store biological data acquired by the following sampling devices such as: van veer grab, box corer, sampling bottles, nets (plankton, trawls and fish), quadrates, and cameras. The DB's logical unit is information regarding a specimen (taxa name, barcode, image), related attributes (abundance, size, age, contaminants…), habitat description, sampling device and method, time and space of sampling, responsible organization and scientist, source of information (cruise, project and publication). The following standardization of specimen and attributes naming were implemented: Taxonomy according to World Register of Marine Species (WoRMS: http://www.marinespecies.org). Habitat description according to Coastal and Marine Ecological Classification Standards (CMECS: http://www.cmecscatalog.org) Parameter name; Unit; Device name; Developmental stage; Institution name; Country name; Marine region according to SeaDataNet Vocabularies (http://www.seadatanet.org/Standards-Software/Common-Vocabularies). This system supports two types of data submission procedures, which support the above stated data structure. The first is a downloadable excel file with drop-down fields based on the ISRAMAR-BIO vocabularies. The file is filled and uploaded online by the data contributor. Alternatively, the same dataset can be assembled by
NASA Technical Reports Server (NTRS)
Sanchez, Hugo; Lewis, Brian; Hanel, Robert
2015-01-01
We are designing and developing a 6U (10 x 22 x 34 cm; 14 kg) nanosatellite as a secondary payload to fly aboard NASAs Space Launch System (SLS) Exploration Mission (EM) 1, scheduled for launch in late 2017. For the first time in over forty years, direct experimental data from biological studies beyond low Earth orbit (LEO) will be obtained during BioSentinels 12- to 18-month mission. BioSentinel will measure the damage and repair of DNA in a biological organism and allow us to compare that to information from onboard physical radiation sensors. In order to understand the relative contributions of the space environments two dominant biological perturbations, reduced gravity and ionizing radiation, results from deep space will be directly compared to data obtained in LEO (on ISS) and on Earth. These data points will be available for validation of existing biological radiation damage and repair models, and for extrapolation to humans, to assist in mitigating risks during future long-term exploration missions beyond LEO. The BioSentinel Payload occupies 4U of the spacecraft and will utilize the monocellular eukaryotic organism Saccharomyces cerevisiae (yeast) to report DNA double-strand-break (DSB) events that result from ambient space radiation. DSB repair exhibits striking conservation of repair proteins from yeast to humans. Yeast was selected because of 1) its similarity to cells in higher organisms, 2) the well-established history of strains engineered to measure DSB repair, 3) its spaceflight heritage, and 4) the wealth of available ground and flight reference data. The S. cerevisiae flight strain will include engineered genetic defects to prevent growth and division until a radiation-induced DSB activates the yeasts DNA repair mechanisms. The triggered culture growth and metabolic activity directly indicate a DSB and its successful repair. The yeast will be carried in the dry state within the 1-atm PL container in 18 separate fluidics cards with each card
BioBrick assembly standards and techniques and associated software tools.
Røkke, Gunvor; Korvald, Eirin; Pahr, Jarle; Oyås, Ove; Lale, Rahmi
2014-01-01
The BioBrick idea was developed to introduce the engineering principles of abstraction and standardization into synthetic biology. BioBricks are DNA sequences that serve a defined biological function and can be readily assembled with any other BioBrick parts to create new BioBricks with novel properties. In order to achieve this, several assembly standards can be used. Which assembly standards a BioBrick is compatible with, depends on the prefix and suffix sequences surrounding the part. In this chapter, five of the most common assembly standards will be described, as well as some of the most used assembly techniques, cloning procedures, and a presentation of the available software tools that can be used for deciding on the best method for assembling of different BioBricks, and searching for BioBrick parts in the Registry of Standard Biological Parts database.
Alkaline hydrothermal liquefaction of swine carcasses to bio-oil
DOE Office of Scientific and Technical Information (OSTI.GOV)
Zheng, Ji-Lu, E-mail: triace@163.com; Zhu, Ming-Qiang; Wu, Hai-tang
Highlights: • Swine carcasses can be converted to bio-oil by alkaline hydrothermal liquefaction. • It seems that the use of the bio-oil for heat or CHP is technically suitable. • Some valuable chemicals were found in the bio-oils. • The bio-oil and the solid residue constituted an energy efficiency of 93.63% for the feedstock. • The solid residue can be used as a soil amendment, to sequester C and for preparing activated carbon. - Abstract: It is imperative that swine carcasses are disposed of safely, practically and economically. Alkaline hydrothermal liquefaction of swine carcasses to bio-oil was performed. Firstly, themore » effects of temperature, reaction time and pH value on the yield of each liquefaction product were determined. Secondly, liquefaction products, including bio-oil and solid residue, were characterized. Finally, the energy recovery ratio (ERR), which was defined as the energy of the resultant products compared to the energy input of the material, was investigated. Our experiment shows that reaction time had certain influence on the yield of liquefaction products, but temperature and pH value had bigger influence on the yield of liquefaction products. Yields of 62.2 wt% bio-oil, having a high heating value of 32.35 MJ/kg and a viscosity of 305cp, and 22 wt% solid residue were realized at a liquefaction temperature of 250 °C, a reaction time of 60 min and a pH value of 9.0. The bio-oil contained up to hundreds of different chemical components that may be classified according to functional groups. Typical compound classes in the bio-oil were hydrocarbons, organic acids, esters, ketones and heterocyclics. The energy recovery ratio (ERR) reached 93.63%. The bio-oil is expected to contribute to fossil fuel replacement in stationary applications, including boilers and furnaces, and upgrading processes for the bio-oil may be used to obtain liquid transport fuels.« less
BioRuby: bioinformatics software for the Ruby programming language.
Goto, Naohisa; Prins, Pjotr; Nakao, Mitsuteru; Bonnal, Raoul; Aerts, Jan; Katayama, Toshiaki
2010-10-15
The BioRuby software toolkit contains a comprehensive set of free development tools and libraries for bioinformatics and molecular biology, written in the Ruby programming language. BioRuby has components for sequence analysis, pathway analysis, protein modelling and phylogenetic analysis; it supports many widely used data formats and provides easy access to databases, external programs and public web services, including BLAST, KEGG, GenBank, MEDLINE and GO. BioRuby comes with a tutorial, documentation and an interactive environment, which can be used in the shell, and in the web browser. BioRuby is free and open source software, made available under the Ruby license. BioRuby runs on all platforms that support Ruby, including Linux, Mac OS X and Windows. And, with JRuby, BioRuby runs on the Java Virtual Machine. The source code is available from http://www.bioruby.org/. katayama@bioruby.org
Security enhanced BioEncoding for protecting iris codes
NASA Astrophysics Data System (ADS)
Ouda, Osama; Tsumura, Norimichi; Nakaguchi, Toshiya
2011-06-01
Improving the security of biometric template protection techniques is a key prerequisite for the widespread deployment of biometric technologies. BioEncoding is a recently proposed template protection scheme, based on the concept of cancelable biometrics, for protecting biometric templates represented as binary strings such as iris codes. The main advantage of BioEncoding over other template protection schemes is that it does not require user-specific keys and/or tokens during verification. Besides, it satisfies all the requirements of the cancelable biometrics construct without deteriorating the matching accuracy. However, although it has been shown that BioEncoding is secure enough against simple brute-force search attacks, the security of BioEncoded templates against more smart attacks, such as record multiplicity attacks, has not been sufficiently investigated. In this paper, a rigorous security analysis of BioEncoding is presented. Firstly, resistance of BioEncoded templates against brute-force attacks is revisited thoroughly. Secondly, we show that although the cancelable transformation employed in BioEncoding might be non-invertible for a single protected template, the original iris code could be inverted by correlating several templates used in different applications but created from the same iris. Accordingly, we propose an important modification to the BioEncoding transformation process in order to hinder attackers from exploiting this type of attacks. The effectiveness of adopting the suggested modification is validated and its impact on the matching accuracy is investigated empirically using CASIA-IrisV3-Interval dataset. Experimental results confirm the efficacy of the proposed approach and show that it preserves the matching accuracy of the unprotected iris recognition system.
Bio-robots automatic navigation with electrical reward stimulation.
Sun, Chao; Zhang, Xinlu; Zheng, Nenggan; Chen, Weidong; Zheng, Xiaoxiang
2012-01-01
Bio-robots that controlled by outer stimulation through brain computer interface (BCI) suffer from the dependence on realtime guidance of human operators. Current automatic navigation methods for bio-robots focus on the controlling rules to force animals to obey man-made commands, with animals' intelligence ignored. This paper proposes a new method to realize the automatic navigation for bio-robots with electrical micro-stimulation as real-time rewards. Due to the reward-seeking instinct and trial-and-error capability, bio-robot can be steered to keep walking along the right route with rewards and correct its direction spontaneously when rewards are deprived. In navigation experiments, rat-robots learn the controlling methods in short time. The results show that our method simplifies the controlling logic and realizes the automatic navigation for rat-robots successfully. Our work might have significant implication for the further development of bio-robots with hybrid intelligence.
Protein bio-corona: critical issue in immune nanotoxicology.
Neagu, Monica; Piperigkou, Zoi; Karamanou, Konstantina; Engin, Ayse Basak; Docea, Anca Oana; Constantin, Carolina; Negrei, Carolina; Nikitovic, Dragana; Tsatsakis, Aristidis
2017-03-01
With the expansion of the nanomedicine field, the knowledge focusing on the behavior of nanoparticles in the biological milieu has rapidly escalated. Upon introduction to a complex biological system, nanomaterials dynamically interact with all the encountered biomolecules and form the protein "bio-corona." The decoration with these surface biomolecules endows nanoparticles with new properties. The present review will address updates of the protein bio-corona characteristics as influenced by nanoparticle's physicochemical properties and by the particularities of the encountered biological milieu. Undeniably, bio-corona generation influences the efficacy of the nanodrug and guides the actions of innate and adaptive immunity. Exploiting the dynamic process of protein bio-corona development in combination with the new engineered horizons of drugs linked to nanoparticles could lead to innovative functional nanotherapies. Therefore, bio-medical nanotechnologies should focus on the interactions of nanoparticles with the immune system for both safety and efficacy reasons.
Brucella BioR regulator defines a complex regulatory mechanism for bacterial biotin metabolism.
Feng, Youjun; Xu, Jie; Zhang, Huimin; Chen, Zeliang; Srinivas, Swaminath
2013-08-01
The enzyme cofactor biotin (vitamin H or B7) is an energetically expensive molecule whose de novo biosynthesis requires 20 ATP equivalents. It seems quite likely that diverse mechanisms have evolved to tightly regulate its biosynthesis. Unlike the model regulator BirA, a bifunctional biotin protein ligase with the capability of repressing the biotin biosynthetic pathway, BioR has been recently reported by us as an alternative machinery and a new type of GntR family transcriptional factor that can repress the expression of the bioBFDAZ operon in the plant pathogen Agrobacterium tumefaciens. However, quite unusually, a closely related human pathogen, Brucella melitensis, has four putative BioR-binding sites (both bioR and bioY possess one site in the promoter region, whereas the bioBFDAZ [bio] operon contains two tandem BioR boxes). This raised the question of whether BioR mediates the complex regulatory network of biotin metabolism. Here, we report that this is the case. The B. melitensis BioR ortholog was overexpressed and purified to homogeneity, and its solution structure was found to be dimeric. Functional complementation in a bioR isogenic mutant of A. tumefaciens elucidated that Brucella BioR is a functional repressor. Electrophoretic mobility shift assays demonstrated that the four predicted BioR sites of Brucella plus the BioR site of A. tumefaciens can all interact with the Brucella BioR protein. In a reporter strain that we developed on the basis of a double mutant of A. tumefaciens (the ΔbioR ΔbioBFDA mutant), the β-galactosidase (β-Gal) activity of three plasmid-borne transcriptional fusions (bioBbme-lacZ, bioYbme-lacZ, and bioRbme-lacZ) was dramatically decreased upon overexpression of Brucella bioR. Real-time quantitative PCR analyses showed that the expression of bioBFDA and bioY is significantly elevated upon removal of bioR from B. melitensis. Together, we conclude that Brucella BioR is not only a negative autoregulator but also a repressor of
Brucella BioR Regulator Defines a Complex Regulatory Mechanism for Bacterial Biotin Metabolism
Xu, Jie; Zhang, Huimin; Srinivas, Swaminath
2013-01-01
The enzyme cofactor biotin (vitamin H or B7) is an energetically expensive molecule whose de novo biosynthesis requires 20 ATP equivalents. It seems quite likely that diverse mechanisms have evolved to tightly regulate its biosynthesis. Unlike the model regulator BirA, a bifunctional biotin protein ligase with the capability of repressing the biotin biosynthetic pathway, BioR has been recently reported by us as an alternative machinery and a new type of GntR family transcriptional factor that can repress the expression of the bioBFDAZ operon in the plant pathogen Agrobacterium tumefaciens. However, quite unusually, a closely related human pathogen, Brucella melitensis, has four putative BioR-binding sites (both bioR and bioY possess one site in the promoter region, whereas the bioBFDAZ [bio] operon contains two tandem BioR boxes). This raised the question of whether BioR mediates the complex regulatory network of biotin metabolism. Here, we report that this is the case. The B. melitensis BioR ortholog was overexpressed and purified to homogeneity, and its solution structure was found to be dimeric. Functional complementation in a bioR isogenic mutant of A. tumefaciens elucidated that Brucella BioR is a functional repressor. Electrophoretic mobility shift assays demonstrated that the four predicted BioR sites of Brucella plus the BioR site of A. tumefaciens can all interact with the Brucella BioR protein. In a reporter strain that we developed on the basis of a double mutant of A. tumefaciens (the ΔbioR ΔbioBFDA mutant), the β-galactosidase (β-Gal) activity of three plasmid-borne transcriptional fusions (bioBbme-lacZ, bioYbme-lacZ, and bioRbme-lacZ) was dramatically decreased upon overexpression of Brucella bioR. Real-time quantitative PCR analyses showed that the expression of bioBFDA and bioY is significantly elevated upon removal of bioR from B. melitensis. Together, we conclude that Brucella BioR is not only a negative autoregulator but also a repressor of
Benchmarking desktop and mobile handwriting across COTS devices: The e-BioSign biometric database
Tolosana, Ruben; Vera-Rodriguez, Ruben; Fierrez, Julian; Morales, Aythami; Ortega-Garcia, Javier
2017-01-01
This paper describes the design, acquisition process and baseline evaluation of the new e-BioSign database, which includes dynamic signature and handwriting information. Data is acquired from 5 different COTS devices: three Wacom devices (STU-500, STU-530 and DTU-1031) specifically designed to capture dynamic signatures and handwriting, and two general purpose tablets (Samsung Galaxy Note 10.1 and Samsung ATIV 7). For the two Samsung tablets, data is collected using both pen stylus and also the finger in order to study the performance of signature verification in a mobile scenario. Data was collected in two sessions for 65 subjects, and includes dynamic information of the signature, the full name and alpha numeric sequences. Skilled forgeries were also performed for signatures and full names. We also report a benchmark evaluation based on e-BioSign for person verification under three different real scenarios: 1) intra-device, 2) inter-device, and 3) mixed writing-tool. We have experimented the proposed benchmark using the main existing approaches for signature verification: feature- and time functions-based. As a result, new insights into the problem of signature biometrics in sensor-interoperable scenarios have been obtained, namely: the importance of specific methods for dealing with device interoperability, and the necessity of a deeper analysis on signatures acquired using the finger as the writing tool. This e-BioSign public database allows the research community to: 1) further analyse and develop signature verification systems in realistic scenarios, and 2) investigate towards a better understanding of the nature of the human handwriting when captured using electronic COTS devices in realistic conditions. PMID:28475590
Benchmarking desktop and mobile handwriting across COTS devices: The e-BioSign biometric database.
Tolosana, Ruben; Vera-Rodriguez, Ruben; Fierrez, Julian; Morales, Aythami; Ortega-Garcia, Javier
2017-01-01
This paper describes the design, acquisition process and baseline evaluation of the new e-BioSign database, which includes dynamic signature and handwriting information. Data is acquired from 5 different COTS devices: three Wacom devices (STU-500, STU-530 and DTU-1031) specifically designed to capture dynamic signatures and handwriting, and two general purpose tablets (Samsung Galaxy Note 10.1 and Samsung ATIV 7). For the two Samsung tablets, data is collected using both pen stylus and also the finger in order to study the performance of signature verification in a mobile scenario. Data was collected in two sessions for 65 subjects, and includes dynamic information of the signature, the full name and alpha numeric sequences. Skilled forgeries were also performed for signatures and full names. We also report a benchmark evaluation based on e-BioSign for person verification under three different real scenarios: 1) intra-device, 2) inter-device, and 3) mixed writing-tool. We have experimented the proposed benchmark using the main existing approaches for signature verification: feature- and time functions-based. As a result, new insights into the problem of signature biometrics in sensor-interoperable scenarios have been obtained, namely: the importance of specific methods for dealing with device interoperability, and the necessity of a deeper analysis on signatures acquired using the finger as the writing tool. This e-BioSign public database allows the research community to: 1) further analyse and develop signature verification systems in realistic scenarios, and 2) investigate towards a better understanding of the nature of the human handwriting when captured using electronic COTS devices in realistic conditions.
Low-voltage 96 dB snapshot CMOS image sensor with 4.5 nW power dissipation per pixel.
Spivak, Arthur; Teman, Adam; Belenky, Alexander; Yadid-Pecht, Orly; Fish, Alexander
2012-01-01
Modern "smart" CMOS sensors have penetrated into various applications, such as surveillance systems, bio-medical applications, digital cameras, cellular phones and many others. Reducing the power of these sensors continuously challenges designers. In this paper, a low power global shutter CMOS image sensor with Wide Dynamic Range (WDR) ability is presented. This sensor features several power reduction techniques, including a dual voltage supply, a selective power down, transistors with different threshold voltages, a non-rationed logic, and a low voltage static memory. A combination of all these approaches has enabled the design of the low voltage "smart" image sensor, which is capable of reaching a remarkable dynamic range, while consuming very low power. The proposed power-saving solutions have allowed the maintenance of the standard architecture of the sensor, reducing both the time and the cost of the design. In order to maintain the image quality, a relation between the sensor performance and power has been analyzed and a mathematical model, describing the sensor Signal to Noise Ratio (SNR) and Dynamic Range (DR) as a function of the power supplies, is proposed. The described sensor was implemented in a 0.18 um CMOS process and successfully tested in the laboratory. An SNR of 48 dB and DR of 96 dB were achieved with a power dissipation of 4.5 nW per pixel.
Low-Voltage 96 dB Snapshot CMOS Image Sensor with 4.5 nW Power Dissipation per Pixel
Spivak, Arthur; Teman, Adam; Belenky, Alexander; Yadid-Pecht, Orly; Fish, Alexander
2012-01-01
Modern “smart” CMOS sensors have penetrated into various applications, such as surveillance systems, bio-medical applications, digital cameras, cellular phones and many others. Reducing the power of these sensors continuously challenges designers. In this paper, a low power global shutter CMOS image sensor with Wide Dynamic Range (WDR) ability is presented. This sensor features several power reduction techniques, including a dual voltage supply, a selective power down, transistors with different threshold voltages, a non-rationed logic, and a low voltage static memory. A combination of all these approaches has enabled the design of the low voltage “smart” image sensor, which is capable of reaching a remarkable dynamic range, while consuming very low power. The proposed power-saving solutions have allowed the maintenance of the standard architecture of the sensor, reducing both the time and the cost of the design. In order to maintain the image quality, a relation between the sensor performance and power has been analyzed and a mathematical model, describing the sensor Signal to Noise Ratio (SNR) and Dynamic Range (DR) as a function of the power supplies, is proposed. The described sensor was implemented in a 0.18 um CMOS process and successfully tested in the laboratory. An SNR of 48 dB and DR of 96 dB were achieved with a power dissipation of 4.5 nW per pixel. PMID:23112588
A brief review of extrusion-based tissue scaffold bio-printing.
Ning, Liqun; Chen, Xiongbiao
2017-08-01
Extrusion-based bio-printing has great potential as a technique for manipulating biomaterials and living cells to create three-dimensional (3D) scaffolds for damaged tissue repair and function restoration. Over the last two decades, advances in both engineering techniques and life sciences have evolved extrusion-based bio-printing from a simple technique to one able to create diverse tissue scaffolds from a wide range of biomaterials and cell types. However, the complexities associated with synthesis of materials for bio-printing and manipulation of multiple materials and cells in bio-printing pose many challenges for scaffold fabrication. This paper presents an overview of extrusion-based bio-printing for scaffold fabrication, focusing on the prior-printing considerations (such as scaffold design and materials/cell synthesis), working principles, comparison to other techniques, and to-date achievements. This paper also briefly reviews the recent development of strategies with regard to hydrogel synthesis, multi-materials/cells manipulation, and process-induced cell damage in extrusion-based bio-printing. The key issue and challenges for extrusion-based bio-printing are also identified and discussed along with recommendations for future, aimed at developing novel biomaterials and bio-printing systems, creating patterned vascular networks within scaffolds, and preserving the cell viability and functions in scaffold bio-printing. The address of these challenges will significantly enhance the capability of extrusion-based bio-printing. Copyright © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Contemporaneous disequilibrium of bio-optical properties in the Southern Ocean
NASA Astrophysics Data System (ADS)
Kahru, Mati; Lee, Zhongping; Mitchell, B. Greg
2017-03-01
Significant changes in satellite-detected net primary production (NPP, mg C m-2 d-1) were observed in the Southern Ocean during 2011-2016: an increase in the Pacific sector and a decrease in the Atlantic sector. While no clear physical forcing was identified, we hypothesize that the changes in NPP were associated with changes in the phytoplankton community and reflected in the concomitant bio-optical properties. Satellite algorithms for chlorophyll a concentration (Chl a, mg m-3) use a combination of estimates of the remote sensing reflectance Rrs(λ) that are statistically fitted to a global reference data set. In any particular region or point in space/time the estimate produced by the global "mean" algorithm can deviate from the true value. Reflectance anomaly (RA) is supposed to remove the first-order variability in Rrs(λ) associated with Chl a and reveal bio-optical properties that are due to the composition of phytoplankton and associated materials. Time series of RA showed variability at multiple scales, including the life span of the sensor, multiyear and annual. Models of plankton functional types using estimated Chl a as input cannot be expected to correctly resolve regional and seasonal anomalies due to biases in the Chl a estimate that they are based on. While a statistical model using RA(λ) time series can predict the times series of NPP with high accuracy (R2 = 0.82) in both Pacific and Atlantic regions, the underlying mechanisms in terms of phytoplankton groups and the associated materials remain elusive.
NASA Astrophysics Data System (ADS)
Tao, Weijun; Zhang, Jianyun; Li, Guangyi; Liu, Tao; Liu, Fengping; Yi, Jingang; Wang, Hesheng; Inoue, Yoshio
2016-02-01
Wearable sensors are attractive for gait analysis because these systems can measure and obtain real-time human gait and motion information outside of the laboratory for a longer duration. In this paper, we present a new wearable ground reaction force (GRF) sensing system for ambulatory gait measurement. In addition, the GRF sensor system is also used to quantify the patients' lower-limb gait rehabilitation. We conduct a validation experiment for the sensor system on seven volunteer subjects (weight 62.39 +/- 9.69 kg and height 169.13 +/- 5.64 cm). The experiments include the use of the GRF sensing system for the subjects in the following conditions: (1) normal walking; (2) walking with the rehabilitation training device; and (3) walking with a knee brace and the rehabilitation training device. The experiment results support the hypothesis that the wearable GRF sensor system is capable of quantifying patients' lower-limb rehabilitation. The proposed GRF sensing system can also be used for assessing the effectiveness of a gait rehabilitation system and for providing bio-feedback information to the subjects.
Geer, Lewis Y; Marchler-Bauer, Aron; Geer, Renata C; Han, Lianyi; He, Jane; He, Siqian; Liu, Chunlei; Shi, Wenyao; Bryant, Stephen H
2010-01-01
The NCBI BioSystems database, found at http://www.ncbi.nlm.nih.gov/biosystems/, centralizes and cross-links existing biological systems databases, increasing their utility and target audience by integrating their pathways and systems into NCBI resources. This integration allows users of NCBI's Entrez databases to quickly categorize proteins, genes and small molecules by metabolic pathway, disease state or other BioSystem type, without requiring time-consuming inference of biological relationships from the literature or multiple experimental datasets.
BioMEMS to bionanotechnology: state of the art in integrated biochips and future prospects
NASA Astrophysics Data System (ADS)
Gupta, Amit; Li, H.; Gomez, Rafael; Chang, W.-J.; Koo, Y. M.; Chang, H.; Andreadakis, G.; Akin, Demir; Bashir, Rashid
2004-12-01
Biomedical or Biological Micro-Electro-Mechanical- Systems (BioMEMS) have in recent years become increasingly prevalent and have found widespread use in a wide variety of applications such as diagnostics, therapeutics and tissue engineering. This paper reviews the interdisciplinary work performed in our group in recent years to develop micro-integrated devices to characterize biological entities. We present the use of electrical and mechanically based phenomena to perform characterization and various functions needed for integrated biochips. One sub-system takes advantage of the dielectrophoretic effect to sort and concentrate bacterial cells and viruses within a micro-fluidic biochip. Another sub-system measures impedance changes produced by the metabolic activity of bacterial cells to determine their viability. A third sub-system is used to detect the mass of viruses as they bind to micro-mechanical sensors. The last sub-system described has been used to detect the charge on DNA molecules as it translocates through nanopore channels. These devices with an electronic or mechanical signal output can be very useful in producing practical systems for rapid detection and characterization of cells for a wide variety of applications in the food safety and health diagnostics industries. The paper will also briefly discuss future prospects of BioMEMS and its possible impact and on bionanotechnology.
Choi, Gyung-Goo; Oh, Seung-Jin; Lee, Soon-Jang; Kim, Joo-Sik
2015-02-01
A fraction of palm kernel shells (PKS) was pyrolyzed in a fluidized bed reactor. The experiments were performed in a temperature range of 479-555 °C to produce bio-oil, biochar, and gas. All the bio-oils were analyzed quantitatively and qualitatively by GC-FID and GC-MS. The maximum content of phenolic compounds in the bio-oil was 24.8 wt.% at ∼500 °C. The maximum phenol content in the bio-oil, as determined by the external standard method, was 8.1 wt.%. A bio-oil derived from the pyrolysis of PKS was used in the synthesis of phenolic resin, showing that the bio-oil could substitute for fossil phenol up to 25 wt.%. The biochar was activated using CO2 at a final activation temperature of 900 °C with different activation time (1-3 h) to produce activated carbon. Activated carbons produced were microporous, and the maximum surface area of the activated carbons produced was 807 m(2)/g. Copyright © 2014 Elsevier Ltd. All rights reserved.
Nano-catalysts for upgrading bio-oil: Catalytic decarboxylation and hydrodeoxygenation
NASA Astrophysics Data System (ADS)
Uemura, Yoshimitsu; Tran, Nga T. T.; Naqvi, Salman Raza; Nishiyama, Norikazu
2017-09-01
Bio-oil is a mixture of oxygenated chemicals produced by fast pyrolysis of lignocellulose, and has attracted much attention recently because the raw material is renewable. Primarily, bio-oil can be used as a replacement of heavy oil. But it is not highly recommended due to bio-oil's inferior properties: high acidity and short shelf life. Upgrading of bio-oil is therefore one of the important technologies nowadays, and is categorized into the two: (A) decrarboxylation/decarbonylation by solid acid catalysts and (B) hydrodeoxygenation (HDO) by metallic catalysts. In our research group, decarboxylation of bio-oil by zeolites and HDO of guaiacol (a model compound of bio-oil) have been investigated. In this paper, recent developments of these upgrading reactions in our research group will be introduced.
Bio-functionalization of conductive textile materials with redox enzymes
NASA Astrophysics Data System (ADS)
Kahoush, M.; Behary, N.; Cayla, A.; Nierstrasz, V.
2017-10-01
In recent years, immobilization of oxidoreductase enzymes on electrically conductive materials has played an important role in the development of sustainable bio-technologies. Immobilization process allows the re-use of these bio-catalysts in their final applications. In this study, different methods of immobilizing redox enzymes on conductive textile materials were used to produce bio-functionalized electrodes. These electrodes can be used for bio-processes and bio-sensing in eco-designed applications in domains such as medicine and pollution control. However, the main challenge facing the stability and durability of these electrodes is the maintenance of the enzymatic activity after the immobilization. Hence, preventing the enzyme’s denaturation and leaching is a critical factor for the success of the immobilization processes.
Thinking Outside of the Blue Marble: Novel Ocean Applications Using the VIIRS Sensor
NASA Technical Reports Server (NTRS)
Vandermeulen, Ryan A.; Arnone, Robert
2016-01-01
While planning for future space-borne sensors will increase the quality, quantity, and duration of ocean observations in the years to come, efforts to extend the limits of sensors currently in orbit can help shed light on future scientific gains as well as associated uncertainties. Here, we present several applications that are unique to the polar orbiting Visual Infrared Imaging Radiometer Suite (VIIRS), each of which challenge the threshold capabilities of the sensor and provide lessons for future missions. For instance, while moderate resolution polar orbiters typically have a one day revisit time, we are able to obtain multiple looks of the same area by focusing on the extreme zenith angles where orbital views overlap, and pair these observations with those from other sensors to create pseudo-geostationary data sets. Or, by exploiting high spatial resolution (imaging) channels and analyzing patterns of synoptic covariance across the visible spectrum, we can obtain higher spatial resolution bio-optical products. Alternatively, non-traditional products can illuminate important biological interactions in the ocean, such as the use of the Day-Night-Band to provide some quantification of phototactic behavior of marine life along light polluted beaches, as well as track the location of marine fishing vessel fleets along ocean fronts. In this talk, we explore ways to take full advantage of the capabilities of existing sensors in order to maximize insights for future missions.
BioNSi: A Discrete Biological Network Simulator Tool.
Rubinstein, Amir; Bracha, Noga; Rudner, Liat; Zucker, Noga; Sloin, Hadas E; Chor, Benny
2016-08-05
Modeling and simulation of biological networks is an effective and widely used research methodology. The Biological Network Simulator (BioNSi) is a tool for modeling biological networks and simulating their discrete-time dynamics, implemented as a Cytoscape App. BioNSi includes a visual representation of the network that enables researchers to construct, set the parameters, and observe network behavior under various conditions. To construct a network instance in BioNSi, only partial, qualitative biological data suffices. The tool is aimed for use by experimental biologists and requires no prior computational or mathematical expertise. BioNSi is freely available at http://bionsi.wix.com/bionsi , where a complete user guide and a step-by-step manual can also be found.
BioData: a national aquatic bioassessment database
MacCoy, Dorene
2011-01-01
BioData is a U.S. Geological Survey (USGS) web-enabled database that for the first time provides for the capture, curation, integration, and delivery of bioassessment data collected by local, regional, and national USGS projects. BioData offers field biologists advanced capabilities for entering, editing, and reviewing the macroinvertebrate, algae, fish, and supporting habitat data from rivers and streams. It offers data archival and curation capabilities that protect and maintain data for the long term. BioData provides the Federal, State, and local governments, as well as the scientific community, resource managers, the private sector, and the public with easy access to tens of thousands of samples collected nationwide from thousands of stream and river sites. BioData also provides the USGS with centralized data storage for delivering data to other systems and applications through automated web services. BioData allows users to combine data sets of known quality from different projects in various locations over time. It provides a nationally aggregated database for users to leverage data from many independent projects that, until now, was not feasible at this scale. For example, from 1991 to 2011, the USGS Idaho Water Science Center collected more than 816 bioassessment samples from 63 sites for the National Water Quality Assessment (NAWQA) Program and more than 477 samples from 39 sites for a cooperative USGS and State of Idaho Statewide Water Quality Network (fig. 1). Using BioData, 20 years of samples collected for both of these projects can be combined for analysis. BioData delivers all of the data using current taxonomic nomenclature, thus relieving users of the difficult and time-consuming task of harmonizing taxonomy among samples collected during different time periods. Fish data are reported using the Integrated Taxonomic Information Service (ITIS) Taxonomic Serial Numbers (TSN's). A simple web-data input interface and self-guided, public data
Geer, Lewis Y.; Marchler-Bauer, Aron; Geer, Renata C.; Han, Lianyi; He, Jane; He, Siqian; Liu, Chunlei; Shi, Wenyao; Bryant, Stephen H.
2010-01-01
The NCBI BioSystems database, found at http://www.ncbi.nlm.nih.gov/biosystems/, centralizes and cross-links existing biological systems databases, increasing their utility and target audience by integrating their pathways and systems into NCBI resources. This integration allows users of NCBI’s Entrez databases to quickly categorize proteins, genes and small molecules by metabolic pathway, disease state or other BioSystem type, without requiring time-consuming inference of biological relationships from the literature or multiple experimental datasets. PMID:19854944
Bio-Manufacturing to market pilot project
DOE Office of Scientific and Technical Information (OSTI.GOV)
Dressen, Tiffaney
The Bio-Manufacturing to Market pilot project was a part of the AMJIAC, the Advanced Manufacturing Jobs and Innovation Accelerator Challenge grant. This internship program set out to further define and enhance the talent pipeline from the University and local Community Colleges to startup culture in East Bay Area, provide undergraduate STEM students with opportunities outside academia, and provide startup companies with much needed talent. Over the 4 year period of performance, the Bio-Manufacturing to Market internship program sponsored 75 undergraduate STEM students who were able to spend anywhere from one to six semesters working with local Bay Area startup companiesmore » and DOE sponsored facilities/programs in the biotech, bio-manufacturing, and biomedical device fields.« less
Soft network composite materials with deterministic and bio-inspired designs
Jang, Kyung-In; Chung, Ha Uk; Xu, Sheng; Lee, Chi Hwan; Luan, Haiwen; Jeong, Jaewoong; Cheng, Huanyu; Kim, Gwang-Tae; Han, Sang Youn; Lee, Jung Woo; Kim, Jeonghyun; Cho, Moongee; Miao, Fuxing; Yang, Yiyuan; Jung, Han Na; Flavin, Matthew; Liu, Howard; Kong, Gil Woo; Yu, Ki Jun; Rhee, Sang Il; Chung, Jeahoon; Kim, Byunggik; Kwak, Jean Won; Yun, Myoung Hee; Kim, Jin Young; Song, Young Min; Paik, Ungyu; Zhang, Yihui; Huang, Yonggang; Rogers, John A.
2015-01-01
Hard and soft structural composites found in biology provide inspiration for the design of advanced synthetic materials. Many examples of bio-inspired hard materials can be found in the literature; far less attention has been devoted to soft systems. Here we introduce deterministic routes to low-modulus thin film materials with stress/strain responses that can be tailored precisely to match the non-linear properties of biological tissues, with application opportunities that range from soft biomedical devices to constructs for tissue engineering. The approach combines a low-modulus matrix with an open, stretchable network as a structural reinforcement that can yield classes of composites with a wide range of desired mechanical responses, including anisotropic, spatially heterogeneous, hierarchical and self-similar designs. Demonstrative application examples in thin, skin-mounted electrophysiological sensors with mechanics precisely matched to the human epidermis and in soft, hydrogel-based vehicles for triggered drug release suggest their broad potential uses in biomedical devices. PMID:25782446
Characterization of Deactivated Bio-oil Hydrotreating Catalysts
DOE Office of Scientific and Technical Information (OSTI.GOV)
Wang, Huamin; Wang, Yong
Deactivation of bio-oil hydrotreating catalysts remains a significant challenge because of the poor quality of pyrolysis bio-oil input for hydrotreating and understanding their deactivation mode is critical to developing improved catalysts and processes. In this research, we developed an understanding of the deactivation of two-step bio-oil hydrotreating catalysts (sulfided Ru/C and sulfided CoMo/C) through detailed characterization of the catalysts using various complimentary analytical techniques. Severe fouling of both catalysts by carbonaceous species was the major form of deactivation, which is consistent with the significant loss of surface area and pore volume of both deactivated catalysts and the significant increase ofmore » the bulk density. Further analysis of the carbonaceous species by thermogravimetric analysis and x-ray photoelectron spectroscopy indicated that the carbonaceous species was formed by condensation reaction of active species such as sugars and sugar derivatives (aldehydes and ketones) in bio-oil feedstock during bio-oil hydrotreating under the conditions and catalysts used. Microscopy results did not show metal sintering of the Ru/C catalyst. However, X-ray diffraction indicated a probable transformation of the highly-active CoMoS phase in the sulfided CoMo/C catalyst to Co8S9 and MoS2 phase with low activity. Loss of the active site by transport of inorganic elements from the bio-oil and the reactor construction material onto the catalyst surface also might be a cause of deactivation as indicated by elemental analysis of spent catalysts.« less
A novel optical fiber biochemical sensor based on long period grating
NASA Astrophysics Data System (ADS)
Mao, Xianhui; Liao, Yanbiao; Zhang, Min; Lai, Shurong; Yin, Haibo
2007-09-01
In this paper, our present work, which aimed at investigating a novel optical fiber biochemical sensor based on long period grating (LPG), is introduced. Biochemical sensor is one of the most attractive fields of sensor research, especially with the development and occurrence of all kinds of novel theory and technology such as LPG. When there is a refraction index periodic perturbation, the guiding mode and cladding mode in LPG couple with each other. This make the LPG is sensitive to the ambient refractive index. This means it can be a novel bio-chemical sensor when it is applied in the fields of biochemistry. After investigating the principle of coupling in LPG, where the formulas of resonance wave length and band width are induced by 3-layer step index model, we developed an optical fiber biochemical sensor. The structure of its probe is designed by coating some function films whose thickness is between several tens and several hundreds nanometers on the cladding of optical fiber. Experiments of monitoring the saline separateness process of Bovine Serum Albumin (BSA) and Mice-Immunoglobulin G (M-IgG) by using the developed LPG sensor have been done. The monitoring indicated that for the BSA, the saline separateness occurs when the saturation is between 50% and 60%, for the M-IgG, the percentage is between 30%-40%. Besides the monitoring, the experiments could also analyze the effects of protein type (different molecule structure), protein consistency and saline saturation to saline separateness. The experimental results show that the optical fiber biochemical sensor based on LPG has many advantages such as simple structure, high sensitivity and miniature. It has a promising future in many research fields and application fields.
Low-cost synchronization of high-speed audio and video recordings in bio-acoustic experiments.
Laurijssen, Dennis; Verreycken, Erik; Geipel, Inga; Daems, Walter; Peremans, Herbert; Steckel, Jan
2018-02-27
In this paper, we present a method for synchronizing high-speed audio and video recordings of bio-acoustic experiments. By embedding a random signal into the recorded video and audio data, robust synchronization of a diverse set of sensor streams can be performed without the need to keep detailed records. The synchronization can be performed using recording devices without dedicated synchronization inputs. We demonstrate the efficacy of the approach in two sets of experiments: behavioral experiments on different species of echolocating bats and the recordings of field crickets. We present the general operating principle of the synchronization method, discuss its synchronization strength and provide insights into how to construct such a device using off-the-shelf components. © 2018. Published by The Company of Biologists Ltd.
Harnessing the potential of the Kinect sensor for psychiatric rehabilitation for stroke survivors.
Zhang, Melvyn W B; Ho, Roger C M
2016-03-04
Dominques et al. in their recent article described how low-cost sensors, such as Microsoft Kinect could be utilized for the measurement of various anthropometric measures. With the recent advances in sensors and sensor based technology, along with the rapid advancement in E-health, Microsoft Kinect has been increasingly recognized by researchers and bioengineers to be a low-cost sensor that could help in the collation of various measurements and various data. A recent systematic review done by Da Gama et al. (2015) have looked into the potential of Kinect in terms of motor rehabilitation. The systematic review highlighted the tremendous potential of the sensors and has clearly stated that there is a need for further studies evaluating its potential for rehabilitation. Zhang et al. (2015) in their recent article have advocated several reasons as to why biosensors are pertinent for stroke rehabilitation. Of note, recent studies done by the World Health Organization have highlighted that stroke is a growing epidemic. Aside to the utilization of smartphone based sensors for stroke rehabilitation, as proposed by Zhang et al. (2015), researchers have also investigated the use of other low cost alternatives, such as Kinect, to facilitate the rehabilitation of stroke survivors. Whilst it may seemed like that has been quite extensive evaluation of the Kinect sensor for stroke rehabilitation, one core area that bio-engineers and researchers have not looked into is that of the psychiatric and mental health issues that might at times arise following a stroke. It is thus the aim of this letter to address how such a sensor could be tapped upon for psychiatric rehabilitation amongst stroke survivors. To this end, the authors have thus conceptualized a game that could help in the cognitive remediation for stroke survivors using low cost Kinect sensors.
Condition sensor system and method
NASA Technical Reports Server (NTRS)
Polhemus, J. T.; Morgan, J. E.; Mandell, A. (Inventor)
1978-01-01
The condition sensor system comprises a condition detector which produces a pulse when a parameter of the monitored condition exceeds a desired threshold. A resettable condition counter counts each pulse. A resettable timer is preset to produce a particular time frame. The counter produces a condition signal when the accumulated number of pulses within the time frame is equal to or greater than a preset count. Control means responsive to the incoming pulses and to the condition signal produce control signals that control utilization devices. After a suitable delay, the last detected pulse simultaneously resets the pulse counter and the timer, and prepares them for sensing another condition occurrence within the time frame. The invention has particular utility in the process of detecting rocking motions of blind people. A controlled, audible, bio-feedback signal is provided which constitutes a warning to the blind person that he is rocking.
Bio-printing cell-laden Matrigel–agarose constructs
Fan, Rong; Piou, Marine; Darling, Evan; Cormier, Denis; Sun, Jun; Wan, Jiandi
2017-01-01
3D printing of biological architectures that mimic the structural and functional features of in vivo tissues is of great interest in tissue engineering and the development of transplantable organ constructs. Printable bio-inks that are compatible with cellular activities play critical roles in the process of 3D bio-printing. Although a variety of hydrogels have been used as bio-inks for 3D bio-printing, they inherit poor mechanical properties and/or the lack of essential protein components that compromise their performance. Here, a hybrid Matrigel–agarose hydrogel system has been demonstrated that possesses both desired rheological properties for bio-printing and biocompatibility for long-term (11 days) cell culture. The agarose component in the hybrid hydrogel system enables the maintenance of 3D-printed structures, whereas Matrigel provides essential microenvironments for cell growth. When human intestinal epithelial HCT116 cells are encapsulated in the printed Matrigel–agarose constructs, high cell viability and proper cell spreading morphology are observed. Given that Matrigel is used extensively for 3D cell culturing, the developed 3D-printable Matrigel–agarose system will open a new way to construct Matrigel-based 3D constructs for cell culture and tissue engineering. PMID:27638155
Photonic sensor opportunities for distributed and wireless systems in security applications
NASA Astrophysics Data System (ADS)
Krohn, David
2006-10-01
There are broad ranges of homeland security sensing applications that can be facilitated by distributed fiber optic sensors and photonics integrated wireless systems. These applications include [1]: Pipeline, (Monitoring, Security); Smart structures (Bridges, Tunnels, Dams, Public spaces); Power lines (Monitoring, Security); Transportation security; Chemical/biological detection; Wide area surveillance - perimeter; and Port Security (Underwater surveillance, Cargo container). Many vital assets which cover wide areas, such as pipeline and borders, are under constant threat of being attacked or breached. There is a rapidly emerging need to be able to provide identification of intrusion threats to such vital assets. Similar problems exit for monitoring the basic infrastructure such as water supply, power utilities, communications systems as well as transportation. There is a need to develop a coordinated and integrated solution for the detection of threats. From a sensor standpoint, consideration must not be limited to detection, but how does detection lead to intervention and deterrence. Fiber optic sensor technology must be compatible with other surveillance technologies such as wireless mote technology to facilitate integration. In addition, the multi-functionality of fiber optic sensors must be expanded to include bio-chemical detection. There have been a number of barriers for the acceptance and broad use of smart fiber optic sensors. Compared to telecommunications, the volume is low. This fact coupled with proprietary and custom specifications has kept the price of fiber optic sensors high. There is a general lack of a manufacturing infrastructure and lack of standards for packaging and reliability. Also, there are several competing technologies; some photonic based and other approaches based on conventional non-photonic technologies.
Potency of bio-charcoal briquette from leather cassava tubers and industrial sludge
NASA Astrophysics Data System (ADS)
Citrasari, Nita; Pinatih, Tety A.; Kuncoro, Eko P.; Soegianto, Agoes; Salamun, Irawan, Bambang
2017-06-01
The purpose of this study was to determine the quality of the bio-charcoal briquette with materials from leather cassava tubers and sludge of wastewater treatment plant. The first, bio-charcoal briquette analized stability test and compressive strength. Then, bio-charcoal briquette with best value analyzed for parameter including moisture content, ash content, calorific content, and burned test. The result briquette quality based on compressive strength for bio-charcoal briquettes carbonated water content between 3.8%-4.5% and non-carbonated bio-charcoal briquettes between 5.2%-7.6%. Bio-charcoal carbonation briquette ash content was between 5.30%-7.40% and non-carbonated bio-charcoal briquettes was between 6.86%-7.46%. Bio-charcoal carbonation levels briquettes heated between 578.2 calories/g-1837.7 calories/g and non carbonatedbio-charcoal briquettes between 858.1 calories/g-891.1 calories/g. Carbonated bio-charcoal burned test was between 48-63 minutes and non-carbonated bio-charcoal was between 22-42 minutes. Emissions resulted from the bio-charcoal briquettes for carbonated and non carbonated composition according to the government regulations ESDM No. 047 of 2006 which, at 128 mg/Nm3 and 139 mg/Nm3.
Bio-oil based biorefinery strategy for the production of succinic acid.
Wang, Caixia; Thygesen, Anders; Liu, Yilan; Li, Qiang; Yang, Maohua; Dang, Dan; Wang, Ze; Wan, Yinhua; Lin, Weigang; Xing, Jianmin
2013-01-01
Succinic acid is one of the key platform chemicals which can be produced via biotechnology process instead of petrochemical process. Biomass derived bio-oil have been investigated intensively as an alternative of diesel and gasoline fuels. Bio-oil could be fractionized into organic phase and aqueous phase parts. The organic phase bio-oil can be easily upgraded to transport fuel. The aqueous phase bio-oil (AP-bio-oil) is of low value. There is no report for its usage or upgrading via biological methods. In this paper, the use of AP-bio-oil for the production of succinic acid was investigated. The transgenic E. coli strain could grow in modified M9 medium containing 20 v/v% AP-bio-oil with an increase in OD from 0.25 to 1.09. And 0.38 g/L succinic acid was produced. With the presence of 4 g/L glucose in the medium, succinic acid concentration increased from 1.4 to 2.4 g/L by addition of 20 v/v% AP-bio-oil. When enzymatic hydrolysate of corn stover was used as carbon source, 10.3 g/L succinic acid was produced. The obtained succinic acid concentration increased to 11.5 g/L when 12.5 v/v% AP-bio-oil was added. However, it decreased to 8 g/L when 50 v/v% AP-bio-oil was added. GC-MS analysis revealed that some low molecular carbon compounds in the AP-bio-oil were utilized by E. coli. The results indicate that AP-bio-oil can be used by E. coli for cell growth and succinic acid production.
Kim, Bongkyu; An, Junyeong; Fapyane, Deby; Chang, In Seop
2015-11-01
The current trend of bio-electrochemical systems is to improve strategies related to their applicability and potential for scaling-up. To date, literature has suggested strategies, but the proposal of correlations between each research field remains insufficient. This review paper provides a correlation based on platform techniques, referred to as bio-electronics platforms (BEPs). These BEPs consist of three platforms divided by scope scale: nano-, micro-, and macro-BEPs. In the nano-BEP, several types of electron transfer mechanisms used by electrochemically active bacteria are discussed. In the micro-BEP, factors affecting the formation of conductive biofilms and transport of electrons in the conductive biofilm are investigated. In the macro-BEP, electrodes and separators in bio-anode are debated in terms of real applications, and a scale-up strategy is discussed. Overall, the challenges of each BEP are highlighted, and potential solutions are suggested. In addition, future research directions are provided and research ideas proposed to develop research interest. Copyright © 2015 Elsevier Ltd. All rights reserved.
NASA Astrophysics Data System (ADS)
Ruffin, Paul; Brantley, Christina; Edwards, Eugene; Hutcheson, Guilford
2006-03-01
The Army Aviation and Missile Research, Development, and Engineering Center (AMRDEC) and the Army Research Laboratory (ARL) have initiated a joint advanced technology demonstration program entitled "Prognostics/Diagnostics for the Future Force (PDFF)" with a key objective of developing low or no power embedded sensor suites for harsh environmental monitoring. The most critical challenge of the program is to specify requirements for the embedded sensor suites which will perform on-board diagnostics, maintain a history of sensor data, and forecast weapon health. The authors are currently collaborating with the PDFF program managers and potential customers to quantify the requirements for remotely operated, micro/nano-technology-based sensors for a host of candidate weapon systems. After requirements are finalized, current micro/nanotechnology-based temperature, humidity, g-shock, vibration and chemical sensors for monitoring the out-gassing of weapons propellant, as well as hazardous gaseous species on the battlefield and in urban environments will be improved to meet the full requirements of the PDFF program. In this paper, performance requirements such as power consumption, reliability, maintainability, survivability, size, and cost, along with the associated technical challenges for micro/nanotechnology-based sensor systems operating in military environments, are discussed. In addition, laboratory results from the design and testing of a wireless sensor array, which was developed using a thin film of functionalized carbon nanotube materials, are presented. Conclusions from the research indicate that the detection of bio-hazardous materials is possible using passive and active wireless sensors based on monitoring the reflected phase from the sensor.
Amphiphilic Bio-molecules/ZnO Interface: Enhancement of Bio-affinity and Dispersibility
NASA Astrophysics Data System (ADS)
Meng, Xiu-Qing; Fang, Yun-Zhang; Wu, Feng-Min
2012-01-01
The dispersibility of bio-molecules such as lecithins on the surface of ZnO nanowires are investigated for biosensor applications. Lecithins can be absorbed on an as-synthesized ZnO nanowire surface in the form of sub-micro sized clusters, while scattering well on those annealed under oxygen atmosphere. Wettability analysis reveals that the as-synthesized ZnO nanowires bear a super-hydrophobic surface, which convents to superhydrophilic after oxygen annealing. First-principles calculations indicate that the adsorption energy of ZnO with water is about 0.2 eV at a distance of 2 Å when it is superhydrophilic, suggesting that lecithin can be absorbed on the hydrophilic surface stably at this distance and the bio-affinity can be enhanced under this condition.
Technical and technological solution for vegetal bio-stimulants obtaining
NASA Astrophysics Data System (ADS)
Anghelache, D. G.; Diaconescu, I.; Pătraşcu, R.
2017-08-01
The paper presents a modern technology for bio fertilizers resulted from waste plant mass after harvesting crops Experimental products were obtained rich in nutrients, but unstable in terms of existing microorganisms. Therefore, they conducted further studies to obtaining bio fungicide herb, so in all investigations undertaken so far in the laboratory, were able to conclude that the introduction of medicinal plant extracts with fungicidal effect into the bio fertilizers obtained by degradation of plant material post-harvest can get various bio-stimulants with nourishing effect upon the plants. Following this technology the paper’s objective is to identify a flux scheme for experimental equipment which can produce as final outcome this type of bio-stimulant. Also, in this work, this equipment will be chosen and will be designed following and obeying to the request of every step of the above technology.
Bio-composites from mycelium reinforced agricultural substrates
USDA-ARS?s Scientific Manuscript database
There is a need for biodegradable alternatives to the inert plastics and expanded foams currently used in in manufacturing processes and device components. The material focused on in this report is a bio-composite patented by Ecovative Design, LLC. The bio-composite utilizes the fungus mycelium to i...
BioWarehouse: a bioinformatics database warehouse toolkit.
Lee, Thomas J; Pouliot, Yannick; Wagner, Valerie; Gupta, Priyanka; Stringer-Calvert, David W J; Tenenbaum, Jessica D; Karp, Peter D
2006-03-23
This article addresses the problem of interoperation of heterogeneous bioinformatics databases. We introduce BioWarehouse, an open source toolkit for constructing bioinformatics database warehouses using the MySQL and Oracle relational database managers. BioWarehouse integrates its component databases into a common representational framework within a single database management system, thus enabling multi-database queries using the Structured Query Language (SQL) but also facilitating a variety of database integration tasks such as comparative analysis and data mining. BioWarehouse currently supports the integration of a pathway-centric set of databases including ENZYME, KEGG, and BioCyc, and in addition the UniProt, GenBank, NCBI Taxonomy, and CMR databases, and the Gene Ontology. Loader tools, written in the C and JAVA languages, parse and load these databases into a relational database schema. The loaders also apply a degree of semantic normalization to their respective source data, decreasing semantic heterogeneity. The schema supports the following bioinformatics datatypes: chemical compounds, biochemical reactions, metabolic pathways, proteins, genes, nucleic acid sequences, features on protein and nucleic-acid sequences, organisms, organism taxonomies, and controlled vocabularies. As an application example, we applied BioWarehouse to determine the fraction of biochemically characterized enzyme activities for which no sequences exist in the public sequence databases. The answer is that no sequence exists for 36% of enzyme activities for which EC numbers have been assigned. These gaps in sequence data significantly limit the accuracy of genome annotation and metabolic pathway prediction, and are a barrier for metabolic engineering. Complex queries of this type provide examples of the value of the data warehousing approach to bioinformatics research. BioWarehouse embodies significant progress on the database integration problem for bioinformatics.
Characterization of bio char derived from tapioca skin
NASA Astrophysics Data System (ADS)
Hasnan, F. I.; Iamail, K. N.; Musa, M.; Jaapar, J.; Alwi, H.; Hamid, K. K. K.
2018-03-01
Pyrolysis of tapioca skin was conducted to produce bio chars in the range between 500°C–800°C. Surface modification treatment were performed on bio chars by using chemicals within 24 hours at 30°C and hot water within 1 hour to enhance the bio char’s adsorption properties according to surface area, pore volume, pore size, crystallinity structure and functional groups. The samples were characterized by using BET, XRD, FTIR and Methylene Blue adsorption. Based on BET result, it showed the surface area increased as the pyrolysis temperature increased followed by pore volume and pore size for S0. The optimum temperature for SNaOH, SHW and SMeOH was at 600°C, 700°C and 800°C with the surface area of 75.9874, 274.5066 and 351.5531 m2/g respectively compared to S0 while SP3HO4 has the worst result since it felt on macroporous structure. The percentage of MB adsorption was followed the size of bio chars surface area. Based on FTIR result, at temperature 500°C to 700°C, the bio chars still have functional groups while at 800°C, many functional groups were diminished due to high temperature struck on them. XRD result showed all the bio chars were amorphous. In conclusion, the best surface modification treatment was by Methanol followed by hot water and Sodium Hydroxide at temperature of 700°C and 800°C while Ortho-Phosphoric acid was the worst one and was not suitable for bio char’s surface modification for adsorption purpose.
Bio-oil based biorefinery strategy for the production of succinic acid
2013-01-01
Background Succinic acid is one of the key platform chemicals which can be produced via biotechnology process instead of petrochemical process. Biomass derived bio-oil have been investigated intensively as an alternative of diesel and gasoline fuels. Bio-oil could be fractionized into organic phase and aqueous phase parts. The organic phase bio-oil can be easily upgraded to transport fuel. The aqueous phase bio-oil (AP-bio-oil) is of low value. There is no report for its usage or upgrading via biological methods. In this paper, the use of AP-bio-oil for the production of succinic acid was investigated. Results The transgenic E. coli strain could grow in modified M9 medium containing 20 v/v% AP-bio-oil with an increase in OD from 0.25 to 1.09. And 0.38 g/L succinic acid was produced. With the presence of 4 g/L glucose in the medium, succinic acid concentration increased from 1.4 to 2.4 g/L by addition of 20 v/v% AP-bio-oil. When enzymatic hydrolysate of corn stover was used as carbon source, 10.3 g/L succinic acid was produced. The obtained succinic acid concentration increased to 11.5 g/L when 12.5 v/v% AP-bio-oil was added. However, it decreased to 8 g/L when 50 v/v% AP-bio-oil was added. GC-MS analysis revealed that some low molecular carbon compounds in the AP-bio-oil were utilized by E. coli. Conclusions The results indicate that AP-bio-oil can be used by E. coli for cell growth and succinic acid production. PMID:23657107
BioGraph: unsupervised biomedical knowledge discovery via automated hypothesis generation
2011-01-01
We present BioGraph, a data integration and data mining platform for the exploration and discovery of biomedical information. The platform offers prioritizations of putative disease genes, supported by functional hypotheses. We show that BioGraph can retrospectively confirm recently discovered disease genes and identify potential susceptibility genes, outperforming existing technologies, without requiring prior domain knowledge. Additionally, BioGraph allows for generic biomedical applications beyond gene discovery. BioGraph is accessible at http://www.biograph.be. PMID:21696594
Bio-inspired Fabrication of Complex Hierarchical Structure in Silicon.
Gao, Yang; Peng, Zhengchun; Shi, Tielin; Tan, Xianhua; Zhang, Deqin; Huang, Qiang; Zou, Chuanping; Liao, Guanglan
2015-08-01
In this paper, we developed a top-down method to fabricate complex three dimensional silicon structure, which was inspired by the hierarchical micro/nanostructure of the Morpho butterfly scales. The fabrication procedure includes photolithography, metal masking, and both dry and wet etching techniques. First, microscale photoresist grating pattern was formed on the silicon (111) wafer. Trenches with controllable rippled structures on the sidewalls were etched by inductively coupled plasma reactive ion etching Bosch process. Then, Cr film was angled deposited on the bottom of the ripples by electron beam evaporation, followed by anisotropic wet etching of the silicon. The simple fabrication method results in large scale hierarchical structure on a silicon wafer. The fabricated Si structure has multiple layers with uniform thickness of hundreds nanometers. We conducted both light reflection and heat transfer experiments on this structure. They exhibited excellent antireflection performance for polarized ultraviolet, visible and near infrared wavelengths. And the heat flux of the structure was significantly enhanced. As such, we believe that these bio-inspired hierarchical silicon structure will have promising applications in photovoltaics, sensor technology and photonic crystal devices.
Chen, Chiung-An; Chen, Shih-Lun; Huang, Hong-Yi; Luo, Ching-Hsing
2012-11-22
In this paper, a low-cost, low-power and high performance micro control unit (MCU) core is proposed for wireless body sensor networks (WBSNs). It consists of an asynchronous interface, a register bank, a reconfigurable filter, a slop-feature forecast, a lossless data encoder, an error correct coding (ECC) encoder, a UART interface, a power management (PWM), and a multi-sensor controller. To improve the system performance and expansion abilities, the asynchronous interface is added for handling signal exchanges between different clock domains. To eliminate the noise of various bio-signals, the reconfigurable filter is created to provide the functions of average, binomial and sharpen filters. The slop-feature forecast and the lossless data encoder is proposed to reduce the data of various biomedical signals for transmission. Furthermore, the ECC encoder is added to improve the reliability for the wireless transmission and the UART interface is employed the proposed design to be compatible with wireless devices. For long-term healthcare monitoring application, a power management technique is developed for reducing the power consumption of the WBSN system. In addition, the proposed design can be operated with four different bio-sensors simultaneously. The proposed design was successfully tested with a FPGA verification board. The VLSI architecture of this work contains 7.67-K gate counts and consumes the power of 5.8 mW or 1.9 mW at 100 MHz or 133 MHz processing rate using a TSMC 0.18 μm or 0.13 μm CMOS process. Compared with previous techniques, this design achieves higher performance, more functions, more flexibility and higher compatibility than other micro controller designs.
Bio-oil Stabilization by Hydrogenation over Reduced Metal Catalysts at Low Temperatures
DOE Office of Scientific and Technical Information (OSTI.GOV)
Wang, Huamin; Lee, Suh-Jane; Olarte, Mariefel V.
Biomass fast pyrolysis integrated with bio-oil upgrading represents a very attractive approach for converting biomass to hydrocarbon transportation fuels. However, the thermal and chemical instability of bio-oils presents significant problems when they are being upgraded, and development of effective approaches for stabilizing bio-oils is critical to the success of the technology. Catalytic hydrogenation to remove reactive species in bio-oil has been considered as one of the most efficient ways to stabilize bio-oil. This paper provides a fundamental understanding of hydrogenation of actual bio-oils over a Ru/TiO2 catalyst under conditions relevant to practical bio-oil hydrotreating processes. Bio-oil feed stocks, bio-oils hydrogenatedmore » to different extents, and catalysts have been characterized to provide insights into the chemical and physical properties of these samples and to understand the correlation of the properties with the composition of the bio-oil and catalysts. The results indicated hydrogenation of various components of the bio-oil, including sugars, aldehydes, ketones, alkenes, aromatics, and carboxylic acids, over the Ru/TiO2 catalyst and 120 to 160oC. Hydrogenation of these species significantly changed the chemical and physical properties of the bio-oil and overall improved its thermal stability, especially by reducing the carbonyl content, which represented the content of the most reactive species (i.e., sugar, aldehydes, and ketones). The change of content of each component in response to increasing hydrogen additions suggests the following bio-oil hydrogenation reaction sequence: sugar conversion to sugar alcohols, followed by ketone and aldehyde conversion to alcohols, followed by alkene and aromatic hydrogenation, and then followed by carboxylic acid hydrogenation to alcohols. Hydrogenation of bio-oil samples with different sulfur contents or inorganic material contents suggested that sulfur poisoning of the reduced Ru metal catalysts
NASA Astrophysics Data System (ADS)
Memon, Sanober F.; Lewis, Elfed; Pembroke, J. Tony; Chowdhry, Bhawani S.
2017-04-01
A novel, low cost and highly sensitive optical fibre probe sensor for concentration measurement of ethanol solvent (C2H5OH) corresponding to bio-ethanol production rate by an algae is reported. The principle of operation of the sensor is based on inter-fibre light coupling through an evanescent field interaction to couple the light between two multimode fibres mounted parallel to each other at a minimum possible separation i.e. < 1mm. The sensor was fabricated using a low cost 1000um plastic optical fibre (POF) and was characterized for real time measurement in the broadband spectrum including visible and near infra-red. The wavelength dependency of this sensor design was also investigated by post processing analysis of real time data and hence the optimum wavelength range determined. The proposed sensor has shown significant response in the range of 0.005 - 0.1 %v/v (%volume/volume or volume concentration) which depicts the high sensitivity for monitoring very minute changes in concentration corresponding refractive index changes of the solution. Numerically, sensor has shown the sensitivity of 21945 intensity counts/%v/v or 109.7 counts per every 0.0050 %v/v.
Bio-Nanobattery Development and Characterization
NASA Technical Reports Server (NTRS)
King, Glen C.; Choi, Sang H.; Chu, Sang-Hyon; Kim, Jae-Woo; Watt, Gerald D.; Lillehei, Peter T.; Park, Yeonjoon; Elliott, James R.
2005-01-01
A bio-nanobattery is an electrical energy storage device that utilizes organic materials and processes on an atomic, or nanometer-scale. The bio-nanobattery under development at NASA s Langley Research Center provides new capabilities for electrical power generation, storage, and distribution as compared to conventional power storage systems. Most currently available electronic systems and devices rely on a single, centralized power source to supply electrical power to a specified location in the circuit. As electronic devices and associated components continue to shrink in size towards the nanometer-scale, a single centralized power source becomes impractical. Small systems, such as these, will require distributed power elements to reduce Joule heating, to minimize wiring quantities, and to allow autonomous operation of the various functions performed by the circuit. Our research involves the development and characterization of a bio-nanobattery using ferritins reconstituted with both an iron core (Fe-ferritin) and a cobalt core (Co-ferritin). Synthesis and characterization of the Co-ferritin and Fe-ferritin electrodes were performed, including reducing capability and the half-cell electrical potentials. Electrical output of nearly 0.5 V for the battery cell was measured. Ferritin utilizing other metallic cores were also considered to increase the overall electrical output. Two dimensional ferritin arrays were produced on various substrates to demonstrate the feasibility of a thin-film nano-scaled power storage system for distributed power storage applications. The bio-nanobattery will be ideal for nanometerscaled electronic applications, due to the small size, high energy density, and flexible thin-film structure. A five-cell demonstration article was produced for concept verification and bio-nanobattery characterization. Challenges to be addressed include the development of a multi-layered thin-film, increasing the energy density, dry-cell bionanobattery
Rao, Akshay; Elara, Mohan Rajesh; Elangovan, Karthikeyan
This paper aims to develop a local path planning algorithm for a bio-inspired, reconfigurable crawling robot. A detailed description of the robotic platform is first provided, and the suitability for deployment of each of the current state-of-the-art local path planners is analyzed after an extensive literature review. The Enhanced Vector Polar Histogram algorithm is described and reformulated to better fit the requirements of the platform. The algorithm is deployed on the robotic platform in crawling configuration and favorably compared with other state-of-the-art local path planning algorithms.
Bio-oils from biomass slow pyrolysis: a chemical and toxicological screening.
Cordella, Mauro; Torri, Cristian; Adamiano, Alessio; Fabbri, Daniele; Barontini, Federica; Cozzani, Valerio
2012-09-15
Bio-oils were produced from bench-scale slow-pyrolysis of three different biomass samples (corn stalks, poplar and switchgrass). Experimental protocols were developed and applied in order to screen their chemical composition. Several hazardous compounds were detected in the bio-oil samples analysed, including phenols, furans and polycyclic aromatic hydrocarbons. A procedure was outlined and applied to the assessment of toxicological and carcinogenic hazards of the bio-oils. The following hazardous properties were considered: acute toxicity; ecotoxicity; chronic toxicity; carcinogenicity. Parameters related to these properties were quantified for each component identified in the bio-oils and overall values were estimated for the bio-oils. The hazard screening carried out for the three bio-oils considered suggested that: (i) hazards to human health could be associated with chronic exposures to the bio-oils; (ii) acute toxic effects on humans and eco-toxic effects on aquatic ecosystems could also be possible in the case of loss of containment; and (iii) bio-oils may present a marginal potential carcinogenicity. The approach outlined allows the collection of screening information on the potential hazards posed by the bio-oils. This can be particularly useful when limited time and analytical resources reduce the possibility to obtain detailed specific experimental data. Copyright © 2012 Elsevier B.V. All rights reserved.
Stabilization of Bio-Oil Fractions for Insertion into Petroleum Refineries
DOE Office of Scientific and Technical Information (OSTI.GOV)
Brown, Robert C.; Smith, Ryan; Wright, Mark
This project is part of a collaboration effort between Iowa State University (ISU), University of Oklahoma (OK) and Pacific Northwest National Laboratory (PNNL). The purpose of this project is to stabilize bio-oil fractions and improve their suitability for insertion into petroleum refineries. Bio-oil from fast pyrolysis of biomass is a complex mixture of unstable organic compounds. These organic compounds react under standard room conditions resulting in increases in bio-oil viscosity and water content – both detrimental for bio-oil storage and transportation. This study employed fractionation and upgrading systems to improve the stability of bio-oil. The fractionation system consists of amore » series of condensers, and electrostatic precipitators designed to separate bio-oil into five fractions: soluble carbohydrates (SF1&2), clean phenolic oligomers (CPO) and middle fraction (SF3&4), light oxygenates (SF5). A two-stage upgrading process was designed to process bio-oil stage fractions into stable products that can be inserted into a refinery. In the upgrading system, heavy and middle bio-oil fractions were upgraded into stable oil via cracking and subsequent hydrodeoxygenation. The light oxygenate fraction was steam reformed to provide a portion of requisite hydrogen for hydroprocessing. Hydrotreating and hydrocracking employed hydrogen from natural gas, fuel gas and light oxygenates reforming. The finished products from this study consist of gasoline- and diesel-blend stock fuels.« less
An ex situ evaluation of TBA- and MTBE-baited bio-traps.
North, Katharine P; Mackay, Douglas M; Annable, Michael D; Sublette, Kerry L; Davis, Greg; Holland, Reef B; Petersen, Daniel; Scow, Kate M
2012-08-01
Aquifer microbial communities can be investigated using Bio-traps(®) ("bio-traps"), passive samplers containing Bio-Sep(®) beads ("bio-beads") that are deployed in monitoring wells to be colonized by bacteria delivered via groundwater flow through the well. When bio-beads are "baited" with organic contaminants enriched in (13)C, stable isotope probing allows assessment of the composition and activity of the microbial community. This study used an ex situ system fed by groundwater continuously extracted from an adjacent monitoring well within an experimentally-created aerobic zone treating a tert-butyl alcohol (TBA) plume. The goal was to evaluate aspects of bio-trap performance that cannot be studied quantitatively in situ. The measured groundwater flow through a bio-trap housing suggests that such traps might typically "sample" about 1.8 L per month. The desorption of TBA or methyl tert-butyl ether (MTBE) bait from bio-traps during a typical deployment duration of 6 weeks was approximately 90% and 45%, respectively, of the total initial bait load, with initially high rate of mass loss that decreased markedly after a few days. The concentration of TBA in groundwater flowing by the TBA-baited bio-beads was estimated to be as high as 3400 mg/L during the first few days, which would be expected to inhibit growth of TBA-degrading microbes. Initial inhibition was also implied for the MTBE-baited bio-trap, but at lower concentrations and for a shorter time. After a few days, concentrations in groundwater flowing through the bio-traps dropped below inhibitory concentrations but remained 4-5 orders of magnitude higher than TBA or MTBE concentrations within the aquifer at the experimental site. Desorption from the bio-beads during ex situ deployment occurred at first as predicted by prior sorption analyses of bio-beads but with apparent hysteresis thereafter, possibly due to mass transfer limitations caused by colonizing microbes. These results suggest that TBA- or MTBE
An ex situ evaluation of TBA- and MTBE-baited bio-traps
North, Katharine P.; Mackay, Douglas M.; Annable, Michael D.; Sublette, Kerry L.; Davis, Greg; Holland, Reef B.; Petersen, Daniel; Scow, Kate M.
2013-01-01
Aquifer microbial communities can be investigated using Bio-traps® (“bio-traps”), passive samplers containing Bio-Sep® beads (“bio-beads”) that are deployed in monitoring wells to be colonized by bacteria delivered via groundwater flow through the well. When bio-beads are “baited” with organic contaminants enriched in 13C, stable isotope probing allows assessment of the composition and activity of the microbial community. This study used an ex situ system fed by groundwater continuously extracted from an adjacent monitoring well within an experimentally-created aerobic zone treating a tert-butyl alcohol (TBA) plume. The goal was to evaluate aspects of bio-trap performance that cannot be studied quantitatively in situ. The measured groundwater flow through a bio-trap housing suggests that such traps might typically “sample” about 1.8 L per month. The desorption of TBA or methyl tert-butyl ether (MTBE) bait from bio-traps during a typical deployment duration of 6 weeks was approximately 90% and 45%, respectively, of the total initial bait load, with initially high rate of mass loss that decreased markedly after a few days. The concentration of TBA in groundwater flowing by the TBA-baited bio-beads was estimated to be as high as 3400 mg/L during the first few days, which would be expected to inhibit growth of TBA-degrading microbes. Initial inhibition was also implied for the MTBE-baited bio-trap, but at lower concentrations and for a shorter time. After a few days, concentrations in groundwater flowing through the bio-traps dropped below inhibitory concentrations but remained 4–5 orders of magnitude higher than TBA or MTBE concentrations within the aquifer at the experimental site. Desorption from the bio-beads during ex situ deployment occurred at first as predicted by prior sorption analyses of bio-beads but with apparent hysteresis thereafter, possibly due to mass transfer limitations caused by colonizing microbes. These results suggest that
Cyanobacteria: A Precious Bio-resource in Agriculture, Ecosystem, and Environmental Sustainability
Singh, Jay Shankar; Kumar, Arun; Rai, Amar N.; Singh, Devendra P.
2016-01-01
Keeping in view, the challenges concerning agro-ecosystem and environment, the recent developments in biotechnology offers a more reliable approach to address the food security for future generations and also resolve the complex environmental problems. Several unique features of cyanobacteria such as oxygenic photosynthesis, high biomass yield, growth on non-arable lands and a wide variety of water sources (contaminated and polluted waters), generation of useful by-products and bio-fuels, enhancing the soil fertility and reducing green house gas emissions, have collectively offered these bio-agents as the precious bio-resource for sustainable development. Cyanobacterial biomass is the effective bio-fertilizer source to improve soil physico-chemical characteristics such as water-holding capacity and mineral nutrient status of the degraded lands. The unique characteristics of cyanobacteria include their ubiquity presence, short generation time and capability to fix the atmospheric N2. Similar to other prokaryotic bacteria, the cyanobacteria are increasingly applied as bio-inoculants for improving soil fertility and environmental quality. Genetically engineered cyanobacteria have been devised with the novel genes for the production of a number of bio-fuels such as bio-diesel, bio-hydrogen, bio-methane, synga, and therefore, open new avenues for the generation of bio-fuels in the economically sustainable manner. This review is an effort to enlist the valuable information about the qualities of cyanobacteria and their potential role in solving the agricultural and environmental problems for the future welfare of the planet. PMID:27148218
Cyanobacteria: A Precious Bio-resource in Agriculture, Ecosystem, and Environmental Sustainability.
Singh, Jay Shankar; Kumar, Arun; Rai, Amar N; Singh, Devendra P
2016-01-01
Keeping in view, the challenges concerning agro-ecosystem and environment, the recent developments in biotechnology offers a more reliable approach to address the food security for future generations and also resolve the complex environmental problems. Several unique features of cyanobacteria such as oxygenic photosynthesis, high biomass yield, growth on non-arable lands and a wide variety of water sources (contaminated and polluted waters), generation of useful by-products and bio-fuels, enhancing the soil fertility and reducing green house gas emissions, have collectively offered these bio-agents as the precious bio-resource for sustainable development. Cyanobacterial biomass is the effective bio-fertilizer source to improve soil physico-chemical characteristics such as water-holding capacity and mineral nutrient status of the degraded lands. The unique characteristics of cyanobacteria include their ubiquity presence, short generation time and capability to fix the atmospheric N2. Similar to other prokaryotic bacteria, the cyanobacteria are increasingly applied as bio-inoculants for improving soil fertility and environmental quality. Genetically engineered cyanobacteria have been devised with the novel genes for the production of a number of bio-fuels such as bio-diesel, bio-hydrogen, bio-methane, synga, and therefore, open new avenues for the generation of bio-fuels in the economically sustainable manner. This review is an effort to enlist the valuable information about the qualities of cyanobacteria and their potential role in solving the agricultural and environmental problems for the future welfare of the planet.
Aydin, Muhsin; Carter-Conger, Jacqueline; Gao, Ning; Gilmore, David F; Ricke, Steven C; Ahn, Soohyoun
2018-04-01
Salmonella is one of major foodborne pathogens and the leading cause of foodborne illness-related hospitalizations and deaths. It is critical to develop a sensitive and rapid detection assay that can identify Salmonella to ensure food safety. In this study, a DNA sensor-based suspension array system of high multiplexing ability was developed to identify eight Salmonella serovars commonly associated with foodborne outbreaks to the serotype level. Each DNA sensor was prepared by activating pre-encoded microspheres with oligonucleotide probes that are targeting virulence genes and serovar-specific regions. The mixture of 12 different types of DNA sensors were loaded into a 96-well microplate and used as a 12-plex DNA sensor array platform. DNA isolated from Salmonella was amplified by multiplex polymerase chain reaction (mPCR), and the presence of Salmonella was determined by reading fluorescent signals from hybridization between probes on DNA sensors and fluorescently labeled target DNA using the Bio-Plex® system. The developed multiplex array was able to detect synthetic DNA at the concentration as low as 100 fM and various Salmonella serovars as low as 100 CFU/mL within 1 h post-PCR. Sensitivity of this assay was further improved to 1 CFU/mL with 6-h enrichment. The array system also correctly and specifically identified serotype of tested Salmonella strains without any cross-reactivity with other common foodborne pathogens. Our results indicate the developed DNA sensor suspension array can be a rapid and reliable high-throughput method for simultaneous detection and molecular identification of common Salmonella serotypes.
A Novel Symmetrical Split Ring Resonator Based on Microstrip for Microwave Sensors
NASA Astrophysics Data System (ADS)
Alahnomi, Rammah A.; Zakaria, Z.; Ruslan, E.; Bahar, Amyrul Azuan Mohd
2016-02-01
In this paper, novel symmetrical split ring resonator (SSRR) is proposed as a suitable component for performance enhancement of microwave sensors. SSRR has been employed for enhancing the insertion loss of the microwave sensors. Using the same device area, we can achieve a high Q-factor of 141.54 from the periphery enhancement using Quasi-linear coupling SSRR, whereas loose coupling SSRR can achieve a Q-factor of 33.98 only. Using Quasi-linear coupling SSRR, the Q-factor is enhanced 4.16 times the loose coupling SSRR using the same device area. After the optimization was made, the SSRR sensor with loose coupling scheme has achieved a very high Qfactor value around 407.34 while quasi-linear scheme has achieved high Q-factor value of 278.78 at the same operating frequency with smaller insertion loss. Spurious passbands at 1st, 2nd, 3rd, and 4th harmonics have been completely suppressed well above -20 dB rejection level without visible changes in the passband filter characteristics. The most significant of using SSRR is to be used for various industrial applications such as food industry, quality control, bio-sensing medicine and pharmacy. The simulation result that Quasi-linear coupling SSRR is a viable candidate for the performance enhancement of microwave sensors has been verified.
The BioCyc collection of microbial genomes and metabolic pathways.
Karp, Peter D; Billington, Richard; Caspi, Ron; Fulcher, Carol A; Latendresse, Mario; Kothari, Anamika; Keseler, Ingrid M; Krummenacker, Markus; Midford, Peter E; Ong, Quang; Ong, Wai Kit; Paley, Suzanne M; Subhraveti, Pallavi
2017-08-17
BioCyc.org is a microbial genome Web portal that combines thousands of genomes with additional information inferred by computer programs, imported from other databases and curated from the biomedical literature by biologist curators. BioCyc also provides an extensive range of query tools, visualization services and analysis software. Recent advances in BioCyc include an expansion in the content of BioCyc in terms of both the number of genomes and the types of information available for each genome; an expansion in the amount of curated content within BioCyc; and new developments in the BioCyc software tools including redesigned gene/protein pages and metabolite pages; new search tools; a new sequence-alignment tool; a new tool for visualizing groups of related metabolic pathways; and a facility called SmartTables, which enables biologists to perform analyses that previously would have required a programmer's assistance. © The Author 2017. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.
Alkaline hydrothermal liquefaction of swine carcasses to bio-oil.
Zheng, Ji-Lu; Zhu, Ming-Qiang; Wu, Hai-tang
2015-09-01
It is imperative that swine carcasses are disposed of safely, practically and economically. Alkaline hydrothermal liquefaction of swine carcasses to bio-oil was performed. Firstly, the effects of temperature, reaction time and pH value on the yield of each liquefaction product were determined. Secondly, liquefaction products, including bio-oil and solid residue, were characterized. Finally, the energy recovery ratio (ERR), which was defined as the energy of the resultant products compared to the energy input of the material, was investigated. Our experiment shows that reaction time had certain influence on the yield of liquefaction products, but temperature and pH value had bigger influence on the yield of liquefaction products. Yields of 62.2wt% bio-oil, having a high heating value of 32.35MJ/kg and a viscosity of 305cp, and 22wt% solid residue were realized at a liquefaction temperature of 250°C, a reaction time of 60min and a pH value of 9.0. The bio-oil contained up to hundreds of different chemical components that may be classified according to functional groups. Typical compound classes in the bio-oil were hydrocarbons, organic acids, esters, ketones and heterocyclics. The energy recovery ratio (ERR) reached 93.63%. The bio-oil is expected to contribute to fossil fuel replacement in stationary applications, including boilers and furnaces, and upgrading processes for the bio-oil may be used to obtain liquid transport fuels. Copyright © 2015 Elsevier Ltd. All rights reserved.
BioWarehouse: a bioinformatics database warehouse toolkit
Lee, Thomas J; Pouliot, Yannick; Wagner, Valerie; Gupta, Priyanka; Stringer-Calvert, David WJ; Tenenbaum, Jessica D; Karp, Peter D
2006-01-01
Background This article addresses the problem of interoperation of heterogeneous bioinformatics databases. Results We introduce BioWarehouse, an open source toolkit for constructing bioinformatics database warehouses using the MySQL and Oracle relational database managers. BioWarehouse integrates its component databases into a common representational framework within a single database management system, thus enabling multi-database queries using the Structured Query Language (SQL) but also facilitating a variety of database integration tasks such as comparative analysis and data mining. BioWarehouse currently supports the integration of a pathway-centric set of databases including ENZYME, KEGG, and BioCyc, and in addition the UniProt, GenBank, NCBI Taxonomy, and CMR databases, and the Gene Ontology. Loader tools, written in the C and JAVA languages, parse and load these databases into a relational database schema. The loaders also apply a degree of semantic normalization to their respective source data, decreasing semantic heterogeneity. The schema supports the following bioinformatics datatypes: chemical compounds, biochemical reactions, metabolic pathways, proteins, genes, nucleic acid sequences, features on protein and nucleic-acid sequences, organisms, organism taxonomies, and controlled vocabularies. As an application example, we applied BioWarehouse to determine the fraction of biochemically characterized enzyme activities for which no sequences exist in the public sequence databases. The answer is that no sequence exists for 36% of enzyme activities for which EC numbers have been assigned. These gaps in sequence data significantly limit the accuracy of genome annotation and metabolic pathway prediction, and are a barrier for metabolic engineering. Complex queries of this type provide examples of the value of the data warehousing approach to bioinformatics research. Conclusion BioWarehouse embodies significant progress on the database integration problem for
Bio-threat preparedness: Need for a paradigm shift.
Jindal, A K; Roy, Kaushik
2014-04-01
India of late has been vulnerable to Chemical, Biological, Radiological and Nuclear (CBRN) threat, on account of its unique geographic position. Biological threat is an imminent threat in the hands of a terrorist. The public health system of our country is overburdened due to its present role and bio-attack response is not a priority area. This paper suggests that as the prime focus is on the CR and N threats in the integrated CBRN preparedness strategy and that specialized and technical forces are needed to deal with a bio-threat; hence there is a need for a paradigm shift in policy. The emerging field of bio-threat needs to be delinked from the joint family of 'CBRN', with consequent structural and functional changes. A separate specialized cadre needs to be formed for dealing with bio-threat, created from the pool of doctors and non-medical scientists from the AFMS and the DRDO. Structural changes are needed in the organization, to bring in the resources of NCDC, New Delhi for enhanced disease surveillance capacity and creation of a bio-threat mitigation node in the AFMC, Pune.
Giardino, Claudia; Bresciani, Mariano; Cazzaniga, Ilaria; Schenk, Karin; Rieger, Patrizia; Braga, Federica; Matta, Erica; Brando, Vittorio E
2014-12-15
In this study we evaluate the capabilities of three satellite sensors for assessing water composition and bottom depth in Lake Garda, Italy. A consistent physics-based processing chain was applied to Moderate Resolution Imaging Spectroradiometer (MODIS), Landsat-8 Operational Land Imager (OLI) and RapidEye. Images gathered on 10 June 2014 were corrected for the atmospheric effects with the 6SV code. The computed remote sensing reflectance (Rrs) from MODIS and OLI were converted into water quality parameters by adopting a spectral inversion procedure based on a bio-optical model calibrated with optical properties of the lake. The same spectral inversion procedure was applied to RapidEye and to OLI data to map bottom depth. In situ measurements of Rrs and of concentrations of water quality parameters collected in five locations were used to evaluate the models. The bottom depth maps from OLI and RapidEye showed similar gradients up to 7 m (r = 0.72). The results indicate that: (1) the spatial and radiometric resolutions of OLI enabled mapping water constituents and bottom properties; (2) MODIS was appropriate for assessing water quality in the pelagic areas at a coarser spatial resolution; and (3) RapidEye had the capability to retrieve bottom depth at high spatial resolution. Future work should evaluate the performance of the three sensors in different bio-optical conditions.
Creating a Bio-Inspired Solution to Prevent Erosion
NASA Astrophysics Data System (ADS)
Reher, R.; Martinez, A.; Cola, J.; Frost, D.
2016-12-01
Through the study of geophysical sciences, lessons can be developed which allow for the introduction of bio-inspired design and art concepts to K-5 elementary students. Students are placed into an engineering mindset in which they must apply the concepts of bio-geotechnics to observe how we can use nature to prevent and abate erosion. Problems are staged for students using realistic engineering scenarios such as erosion prevention through biomimicry and the study of anchorage characteristics of root structures in regard to stability of soil. Specifically, a lesson is introduced where students research, learn, and present information about bio-inspired designs to understand these concepts. They lean how plant roots differ in size and shape to stabilize soil. In addition, students perform a series of hands-on experiments which demonstrate how bio-cements and roots can slow erosion.
Bioengineered-inorganic nanosystems for nanophotonics and bio-nanotechnology
NASA Astrophysics Data System (ADS)
Leong, Kirsty; Zin, Melvin T.; Ma, Hong; Huang, Fei; Sarikaya, Mehmet; Jen, Alex K.
2008-08-01
Here we nanoengineered tunable quantum dot and cationic conjugated polymer nanoarrays based on surface plasmon enhanced fluorescence where we achieved a 15-fold and 25-fold increase in their emission intensities, respectively. These peptide mediated hybrid systems were fabricated by horizontally tuning the localized surface plasmon resonance of gold nanoarrays and laterally tuning the distance of the fluorophore from the metal surface. This approach permits a comprehensive control both laterally (i.e., lithographically defined gold nanoarrays) and vertically (i.e., QD/CCP-metal distance) of the collectively behaving QD-NP and CP-NP assemblies by way of biomolecular recognition. The highest photoluminescence was achieved when the quantum dots and cationic conjugated polymers were self-assembled at a distance of 16.00 nm and 18.50 nm from the metal surface, respectively. Specifically, we demonstrated the spectral tuning of plasmon resonant metal nanoarrays and the self-assembly of protein-functionalized QDs/CCPs in a step-wise fashion with a concomitant incremental increase in separation from the metal surface through biotin-streptavidin spacer units. These well-controlled self-assembled patterned arrays provide highly organized architectures for improving optoelectronic devices and/or increasing the sensitivity of bio-chemical sensors.
NASA Astrophysics Data System (ADS)
Birkholz, M.; Ehwald, K.-E.; Basmer, T.; Kulse, P.; Reich, C.; Drews, J.; Genschow, D.; Haak, U.; Marschmeyer, S.; Matthus, E.; Schulz, K.; Wolansky, D.; Winkler, W.; Guschauski, T.; Ehwald, R.
2013-06-01
The progressive scaling in semiconductor technology allows for advanced miniaturization of intelligent systems like implantable biosensors for low-molecular weight analytes. A most relevant application would be the monitoring of glucose in diabetic patients, since no commercial solution is available yet for the continuous and drift-free monitoring of blood sugar levels. We report on a biosensor chip that operates via the binding competition of glucose and dextran to concanavalin A. The sensor is prepared as a fully embedded micro-electromechanical system and operates at GHz frequencies. Glucose concentrations derive from the assay viscosity as determined by the deflection of a 50 nm TiN actuator beam excited by quasi-electrostatic attraction. The GHz detection scheme does not rely on the resonant oscillation of the actuator and safely operates in fluidic environments. This property favorably combines with additional characteristics—(i) measurement times of less than a second, (ii) usage of biocompatible TiN for bio-milieu exposed parts, and (iii) small volume of less than 1 mm3—to qualify the sensor chip as key component in a continuous glucose monitor for the interstitial tissue.
Heterogeneous Deformable Modeling of Bio-Tissues and Haptic Force Rendering for Bio-Object Modeling
NASA Astrophysics Data System (ADS)
Lin, Shiyong; Lee, Yuan-Shin; Narayan, Roger J.
This paper presents a novel technique for modeling soft biological tissues as well as the development of an innovative interface for bio-manufacturing and medical applications. Heterogeneous deformable models may be used to represent the actual internal structures of deformable biological objects, which possess multiple components and nonuniform material properties. Both heterogeneous deformable object modeling and accurate haptic rendering can greatly enhance the realism and fidelity of virtual reality environments. In this paper, a tri-ray node snapping algorithm is proposed to generate a volumetric heterogeneous deformable model from a set of object interface surfaces between different materials. A constrained local static integration method is presented for simulating deformation and accurate force feedback based on the material properties of a heterogeneous structure. Biological soft tissue modeling is used as an example to demonstrate the proposed techniques. By integrating the heterogeneous deformable model into a virtual environment, users can both observe different materials inside a deformable object as well as interact with it by touching the deformable object using a haptic device. The presented techniques can be used for surgical simulation, bio-product design, bio-manufacturing, and medical applications.
Bio-Security Proficiencies Project for Beginning Producers in 4-H
ERIC Educational Resources Information Center
Smith, Martin H.; Meehan, Cheryl L.; Borba, John A.
2014-01-01
Improving bio-security practices among 4-H members who raise and show project animals is important. Bio-security measures can reduce the risk of disease spread and mitigate potential health and economic risks of disease outbreaks involving animal and zoonotic pathogens. Survey data provided statistical evidence that the Bio-Security Proficiencies…
Bio-Monitoring of Ozone by Young Students
ERIC Educational Resources Information Center
Lorenzini, Giacomo; Nali, Cristina
2004-01-01
An educational pilot project on the bio-monitoring of air quality was carried out in the Umbria Region of Central Italy. It involved about 1000 young students (ages 4 to 16) from 42 schools of 16 municipalities in active biomonitoring of tropospheric ozone with bio-indicator sensitive tobacco seedlings. Some 6500 raw biological readings were used…
Recent progress in making protein microarray through BioLP
NASA Astrophysics Data System (ADS)
Yang, Rusong; Wei, Lian; Feng, Ying; Li, Xiujian; Zhou, Quan
2017-02-01
Biological laser printing (BioLP) is a promising biomaterial printing technique. It has the advantage of high resolution, high bioactivity, high printing frequency and small transported liquid amount. In this paper, a set of BioLP device is design and made, and protein microarrays are printed by this device. It's found that both laser intensity and fluid layer thickness have an influence on the microarrays acquired. Besides, two kinds of the fluid layer coating methods are compared, and the results show that blade coating method is better than well-coating method in BioLP. A microarray of 0.76pL protein microarray and a "NUDT" patterned microarray are printed to testify the printing ability of BioLP.
Chien, Po-Jen; Suzuki, Takuma; Tsujii, Masato; Ye, Ming; Toma, Koji; Arakawa, Takahiro; Iwasaki, Yasuhiko; Mitsubayashi, Kohji
2017-05-15
Exhaled breath analysis has attracted lots of researchers attention in the past decades due to its advantages such as its non-invasive property and the possibility of continuous monitoring. In addition, several volatile organic compounds in breath have been identified as biomarkers for some diseases. Particularly, studies have pointed out that concentration of isopropanol (IPA) in exhaled air might relate with certain illnesses such as liver disease, chronic obstructive pulmonary (COPD), and lung cancer. In this study, a highly sensitive and selective biochemical gas sensor (bio-sniffer) for the breath IPA concentration determination was constructed and optimized. This bio-sniffer measures the concentration of IPA according to the fluorescence intensity of oxidized nicotinamide adenine dinucleotide (NADH), which was produced by an enzymatic reaction of secondary alcohol dehydrogenase (S-ADH). The NADH detection system employed an UV-LED as the excitation light, and a highly sensitive photomultiplier tube (PMT) as a fluorescence intensity detector. A gas-sensing region was developed using an optical fiber probe equipped with a flow-cell and enzyme immobilized membrane, and connected to the NADH measurement system. The calibration range of the IPA bio-sniffer was confirmed from 1ppb to 9060ppb that was comparable to other IPA analysis methods. The results of the analysis of breath IPA concentration in healthy subjects using the bio-sniffer showed a mean concentration of 16.0ppb, which was similar to other studies. These results have demonstrated that this highly sensitive and selective bio-sniffer could be used to measure the IPA in exhaled air, and it is expected to apply for breath IPA research and investigation of biomarkers for clinical diagnosis. Copyright © 2016 Elsevier B.V. All rights reserved.
Energy scavenging for long-term deployable wireless sensor networks.
Mathúna, Cian O; O'Donnell, Terence; Martinez-Catala, Rafael V; Rohan, James; O'Flynn, Brendan
2008-05-15
side would be sufficient to supply the complete wireless sensor node energy. Key research challenges to be addressed to deliver future, remote, wireless, chemo-biosensing systems include the development of low cost, low-power sensors, miniaturised fluidic transport systems, anti-bio-fouling sensor surfaces, sensor calibration, reliable and robust system packaging, as well as associated energy delivery systems and energy budget management.
Advancing sustainable forestry by using engineered wood or bio-composites
Jerrold E. Winandy
2005-01-01
As worldwide demand for timber and bio-fiber resources grows, sustainable resource management and industrial utilization must collaborate to develop a shared vision for both long-term sustainable management of forest and bio-resources and sustainable economic development. Engineered wood- and bio-composites offer a tool that can both achieve resource sustainability and...
[Research progress on the technique and materials for three-dimensional bio-printing].
Yang, Runhuai; Chen, Yueming; Ma, Changwang; Wang, Huiqin; Wang, Shuyue
2017-04-01
Three-dimensional (3D) bio-printing is a novel engineering technique by which the cells and support materials can be manufactured to a complex 3D structure. Compared with other 3D printing methods, 3D bio-printing should pay more attention to the biocompatible environment of the printing methods and the materials. Aimed at studying the feature of the 3D bio-printing, this paper mainly focuses on the current research state of 3D bio-printing, with the techniques and materials of the bio-printing especially emphasized. To introduce current printing methods, the inkjet method, extrusion method, stereolithography skill and laser-assisted technique are described. The printing precision, process, requirements and influence of all the techniques on cell status are compared. For introduction of the printing materials, the cross-link, biocompatibility and applications of common bio-printing materials are reviewed and compared. Most of the 3D bio-printing studies are being remained at the experimental stage up to now, so the review of 3D bio-printing could improve this technique for practical use, and it could also contribute to the further development of 3D bio-printing.
Magar, Kaman Thapa; Reich, Gregory W; Kondash, Corey; Slinker, Keith; Pankonien, Alexander M; Baur, Jeffery W; Smyers, Brian
2016-11-10
Distributed arrays of artificial hair sensors have bio-like sensing capabilities to obtain spatial and temporal surface flow information which is an important aspect of an effective fly-by-feel system. The spatiotemporal surface flow measurement enables further exploration of additional flow features such as flow stagnation, separation, and reattachment points. Due to their inherent robustness and fault tolerant capability, distributed arrays of hair sensors are well equipped to assess the aerodynamic and flow states in adverse conditions. In this paper, a local flow measurement from an array of artificial hair sensors in a wind tunnel experiment is used with a feedforward artificial neural network to predict aerodynamic parameters such as lift coefficient, moment coefficient, free-stream velocity, and angle of attack on an airfoil. We find the prediction error within 6% and 10% for lift and moment coefficients. The error for free-stream velocity and angle of attack were within 0.12 mph and 0.37 degrees. Knowledge of these parameters are key to finding the real time forces and moments which paves the way for effective control design to increase flight agility, stability, and maneuverability.
NASA Technical Reports Server (NTRS)
Padgen, Michael R.
2017-01-01
Manned missions beyond low Earth orbit (LEO) require that several strategic knowledge gaps about the effects of space travel on the human body be addressed. NASA Ames Research Center has been the leader in developing autonomous bio nanosatellites, including past successful missions for GeneSat, PharmaSat, and O/OREOS, that tackled some of these issues. These nanosatellites provide in situ measurements, which deliver insight into the dynamic changes in cell behavior in microgravity. In this talk, two upcoming bio nanosatellites developed at Ames, the E. coli Antimicrobial Satellite (EcAMSat) and BioSentinel, will be discussed. Both satellites contain microfluidic systems that precisely deliver nutrients to the microorganisms stored within wells of fluidic cards. Each well, in turn, has its own 3-color LED and detector system which is used to monitor changes in metabolic activity with alamarBlue, a redox indicator, and the optical density of the cells. EcAMSat investigates the effects of microgravity on bacterial resistance to antimicrobial drugs, vital knowledge for understanding how to maintain the health of astronauts in long-term and beyond LEO spaceflight. The behavior of wild type and mutant uropathic E. coli will be compared in microgravity and with ground data to help understand the molecular mechanisms behind antibiotic resistance and how these phenotypes might change in space. BioSentinel seeks to directly measure the effects of space radiation on budding yeast S. cerevisiae, particularly double strand breaks (DSB). While hitching a ride on the SLS EM-1 mission (Orion's first unmanned mission to the moon) in 2018, BioSentinel will be kicked off and enter into a heliocentric orbit, becoming the first study of the effects of radiation on living organisms outside LEO since the Apollo program. The yeast are stored in eighteen independent 16-well microfluidic cards, which will be individually activated over the 12 month mission duration. In addition to the wild
NASA Technical Reports Server (NTRS)
Padgen, Mike
2017-01-01
Manned missions beyond low Earth orbit (LEO) require that several strategic knowledge gaps about the effects of space travel on the human body be addressed. NASA Ames Research Center has been the leader in developing autonomous bio nanosatellites, including past successful missions for GeneSat, PharmaSat, and OOREOS, that tackled some of these issues. These nanosatellites provide in situ measurements, which deliver insight into the dynamic changes in cell behavior in microgravity. In this talk, two upcoming bio nanosatellites developed at Ames, the E. coli Antimicrobial Satellite (EcAMSat) and BioSentinel, will be discussed. Both satellites contain microfluidic systems that precisely deliver nutrients to the microorganisms stored within wells of fluidic cards. Each well, in turn, has its own 3-color LED and detector system which is used to monitor changes in metabolic activity with alamarBlue, a redox indicator, and the optical density of the cells. EcAMSat investigates the effects of microgravity on bacterial resistance to antimicrobial drugs, vital knowledge for understanding how to maintain the health of astronauts in long-term and beyond LEO spaceflight. The behavior of wild type and mutant uropathic E. coli will be compared in microgravity and with ground data to help understand the molecular mechanisms behind antibiotic resistance and how these phenotypes might change in space. BioSentinel seeks to directly measure the effects of space radiation on budding yeast S. cerevisiae, particularly double strand breaks (DSB). While hitching a ride on the SLS EM-1 mission (Orions first unmanned mission to the moon) in 2018, BioSentinel will be kicked off and enter into a heliocentric orbit, becoming the first study of the effects of radiation on living organisms outside LEO since the Apollo program. The yeast are stored in eighteen independent 16-well microfluidic cards, which will be individually activated over the 12 month mission duration. In addition to the wild
Computing through Scientific Abstractions in SysBioPS
DOE Office of Scientific and Technical Information (OSTI.GOV)
Chin, George; Stephan, Eric G.; Gracio, Deborah K.
2004-10-13
Today, biologists and bioinformaticists have a tremendous amount of computational power at their disposal. With the availability of supercomputers, burgeoning scientific databases and digital libraries such as GenBank and PubMed, and pervasive computational environments such as the Grid, biologists have access to a wealth of computational capabilities and scientific data at hand. Yet, the rapid development of computational technologies has far exceeded the typical biologist’s ability to effectively apply the technology in their research. Computational sciences research and development efforts such as the Biology Workbench, BioSPICE (Biological Simulation Program for Intra-Cellular Evaluation), and BioCoRE (Biological Collaborative Research Environment) are importantmore » in connecting biologists and their scientific problems to computational infrastructures. On the Computational Cell Environment and Heuristic Entity-Relationship Building Environment projects at the Pacific Northwest National Laboratory, we are jointly developing a new breed of scientific problem solving environment called SysBioPSE that will allow biologists to access and apply computational resources in the scientific research context. In contrast to other computational science environments, SysBioPSE operates as an abstraction layer above a computational infrastructure. The goal of SysBioPSE is to allow biologists to apply computational resources in the context of the scientific problems they are addressing and the scientific perspectives from which they conduct their research. More specifically, SysBioPSE allows biologists to capture and represent scientific concepts and theories and experimental processes, and to link these views to scientific applications, data repositories, and computer systems.« less
The electromagnetic bio-field: clinical experiments and interferences.
Burnei, G; Hodorogea, D; Georgescu, I; Gavriliu, Ş; Drăghici, I; Dan, D; Vlad, C; Drăghici, L
2012-06-12
One of the most important factors is the technical and scientifically rapid development that is continually modifying the world we live in and polluting it with electromagnetic radiations. A functional and structural influence of magnetic and electromagnetic field on living organisms is presented in the literature by many performed experiments. The notion of bio-field represents the electromagnetic field generated by the bio-structures, not only in their normal physiological activities but also in their pathological states. There is a tight interdependency between the bio-field and the bio-structure, which respects the primary notion of an electromagnetic field given by the Maxwell-Faraday laws, in which, the electromagnetic phenomena are simplified to the field variations. These variations can be expressed in a coherent differential equation system that bounds the field vectors to different space points at different time moments. The living organisms cannot contain electrostatic and magneto-static fields due to the intense activity of the bio-structures. The biochemical reactions that have high rhythms and speeds always impose the electrodynamics character of the biologic field that also corresponds to the stability of the protein molecule that can be explained only through a dynamic way. The existent energy is not considered an exciting agent, and it does not lead to any effects. The parameters of these elementary bio-fields cannot yet be fully known due to technical reasons. The biological structures are very complex ones and undergo continuous dynamical activity. That is why the calculus model should be related to the constant dynamics, nowadays being very difficult to express.
Starch-based bio-elastomers functionalized with red beetroot natural antioxidant.
Tran, Thi Nga; Athanassiou, Athanassia; Basit, Abdul; Bayer, Ilker S
2017-02-01
Red beetroot (RB) powder was incorporated into starch-based bio-elastomers to obtain flexible biocomposites with tunable antioxidant properties. Starch granules within the bio-elastomers affected the release of the antioxidant molecule betanin in the RB powder. The bio-elastomers were hydrophobic and resisted dissolution in water, hence the release of betanin was due to diffusion rather than polymer matrix disintegration. Hydrophobicity was maintained even after water immersion. Released betanin demonstrated highly efficient antioxidant scavenging activity against 2,2-diphenyl-1-picrylhydrazyl free radical (DPPH) and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation (ABTS(+)). RB powder was also found to increase the Young's modulus of the bio-elastomers without compromising their elongation ability. Infrared spectral analysis indicated weak interactions through hydrogen bonding among starch granules, RB powder and PDMS polymer within the bio-elastomers. Hence, as a simple but intelligent biomaterial consisting of mainly edible starch and RB powder the present bio-elastomers can be used in active packaging for a variety of pharmaceutical, medical, and food applications. Copyright © 2016 Elsevier Ltd. All rights reserved.
A Systems Approach to Bio-Oil Stabilization - Final Technical Report
DOE Office of Scientific and Technical Information (OSTI.GOV)
Brown, Robert C; Meyer, Terrence; Fox, Rodney
2011-12-23
The objective of this project is to develop practical, cost effective methods for stabilizing biomass-derived fast pyrolysis oil for at least six months of storage under ambient conditions. The U.S. Department of Energy has targeted three strategies for stabilizing bio-oils: (1) reducing the oxygen content of the organic compounds comprising pyrolysis oil; (2) removal of carboxylic acid groups such that the total acid number (TAN) of the pyrolysis oil is dramatically reduced; and (3) reducing the charcoal content, which contains alkali metals known to catalyze reactions that increase the viscosity of bio-oil. Alkali and alkaline earth metals (AAEM), are knownmore » to catalyze decomposition reactions of biomass carbohydrates to produce light oxygenates that destabilize the resulting bio-oil. Methods envisioned to prevent the AAEM from reaction with the biomass carbohydrates include washing the AAEM out of the biomass with water or dilute acid or infusing an acid catalyst to passivate the AAEM. Infusion of acids into the feedstock to convert all of the AAEM to salts which are stable at pyrolysis temperatures proved to be a much more economically feasible process. Our results from pyrolyzing acid infused biomass showed increases in the yield of anhydrosugars by greater than 300% while greatly reducing the yield of light oxygenates that are known to destabilize bio-oil. Particulate matter can interfere with combustion or catalytic processing of either syngas or bio-oil. It also is thought to catalyze the polymerization of bio-oil, which increases the viscosity of bio-oil over time. High temperature bag houses, ceramic candle filters, and moving bed granular filters have been variously suggested for syngas cleaning at elevated temperatures. High temperature filtration of bio-oil vapors has also been suggested by the National Renewable Energy Laboratory although there remain technical challenges to this approach. The fast pyrolysis of biomass yields three main organic
Wearable dry sensors with bluetooth connection for use in remote patient monitoring systems.
Gargiulo, Gaetano; Bifulco, Paolo; Cesarelli, Mario; Jin, Craig; McEwan, Alistair; van Schaik, Andre
2010-01-01
Cost reduction has become the primary theme of healthcare reforms globally. More providers are moving towards remote patient monitoring, which reduces the length of hospital stays and frees up their physicians and nurses for acute cases and helps them to tackle staff shortages. Physiological sensors are commonly used in many human specialties e.g. electrocardiogram (ECG) electrodes, for monitoring heart signals, and electroencephalogram (EEG) electrodes, for sensing the electrical activity of the brain, are the most well-known applications. Consequently there is a substantial unmet need for physiological sensors that can be simply and easily applied by the patient or primary carer, are comfortable to wear, can accurately sense parameters over long periods of time and can be connected to data recording systems using Bluetooth technology. We have developed a small, battery powered, user customizable portable monitor. This prototype is capable of recording three-axial body acceleration, skin temperature, and has up to four bio analogical front ends. Moreover, it is also able of continuous wireless transmission to any Bluetooth device including a PDA or a cellular phone. The bio-front end can use long-lasting dry electrodes or novel textile electrodes that can be embedded in clothes. The device can be powered by a standard mobile phone which has a Ni-MH 3.6 V battery, to sustain more than seven days continuous functioning when using the Bluetooth Sniff mode to reduce TX power. In this paper, we present some of the evaluation experiments of our wearable personal monitor device with a focus on ECG applications.
NASA Astrophysics Data System (ADS)
White, Ian M.; Oveys, Hesam; Fan, Xudong
2006-02-01
Optical microsphere resonators can function as highly sensitive bio/chemical sensors due to the large Q-factor, which leads to high light-matter interaction. The whispering gallery modes (WGM) arise at the surface of the microsphere, creating a highly enhanced optical field that interacts with matter on or near the microsphere surface. As a result, the spectral position of the WGM is extremely sensitive to refractive index changes near the surface, such as when bio/chemical molecules bind to the sphere. We show the potential feasibility of a microsphere ring resonator as a sensor for small molecules by demonstrating detection of sub-femtomole changes in SiO II molecules at the surface of the microsphere. In this experiment, the silica molecules act as an excellent model for small molecule analytes because of their 60 Dalton molecular weight, and because we know nearly the exact quantity of molecules at the surface, which enables a sensitivity characterization. We measure the spectral shifts in the WGMs when low concentrations of hydrofluoric acid (HF) are added to a solution that is being probed by the microsphere. As the HF molecules break apart the SiO II molecules at the sphere surface, the WGMs shift due to the sub-nano-scale decrease in the size of the microsphere. These calculations show that the sensitivity of this microsphere resonator is on the order of 500 attomoles. Our results will lead to the utilization of optical microspheres for detection of trace quantities of small molecules for such applications as drug discovery, environmental monitoring, and enzyme detection using peptide cleavage.
Opportunity and development of bio-based composites
Zhiyong Cai; Jerrold E. Winandy
2005-01-01
Our forests are a naturally renewable resource that has been used as a principal source of bio-energy and building materials for centuries. The rapid growth of world population has now resulted in substantial increases in demand and in consumption of all raw materials. This now provides a unique opportunity of developing new bio-based composites. The 100-year history...
Liang, Yi; Zhao, Xuefei; Chi, Zhanyou; Rover, Marjorie; Johnston, Patrick; Brown, Robert; Jarboe, Laura; Wen, Zhiyou
2013-04-01
This work was to utilize acetic acid contained in bio-oil for growth and lipid production of the microalga Chlamydomonas reinhardtii. The acetic acid-rich bio-oil fraction derived from fast pyrolysis of softwood contained 26% (w/w) acetic acid, formic acid, methanol, furfural, acetol, and phenolics as identified compounds, and 13% (w/w) unidentified compounds. Among those identified compounds, phenolics were most inhibitory to algal growth, followed by furfural and acetol. To enhance the fermentability of the bio-oil fraction, activated carbon was used to reduce the toxicity of the bio-oil, while metabolic evolution was used to enhance the toxicity tolerance of the microalgae. Combining activated carbon treatment and using evolved algal strain resulted in significant algal growth improvement. The results collectively showed that fast pyrolysis-fermentation process was a viable approach for converting biomass into fuels and chemicals. Copyright © 2013 Elsevier Ltd. All rights reserved.
What can law do for the development of bio-economy?
Chang-Qiu, Liu
2012-03-01
Bio-technology has become a new impeller to the development of the world economy since the 1970's. The development of bio-economy has two sides for mankind which calls for intervention by law. During the legislation of bioeconomy, some special principles should be esteemed and observed by legislators. It is necessary for the healthy development of bio-economy.
Bio-methane from an-aerobic digestion using activated carbon adsorption.
Farooq, Muhammad; Bell, Alexandra H; Almustapha, M N; Andresen, John M
2017-08-01
There is an increasing global demand for carbon-neutral bio-methane from an-aerobic digestion (AD) to be injected into national gas grids. Bio-gas, a methane -rich energy gas, is produced by microbial decomposition of organic matter through an-aerobic conditions where the presence of carbon dioxide and hydrogen sulphide affects its performance. Although the microbiological process in the AD can be tailored to enhance the bio-gas composition, physical treatment is needed to convert the bio-gas into bio-methane. Water washing is the most common method for upgrading bio-gas for bio-methane production, but its large use of water is challenging towards industrial scale-up. Hence, the present study focuses on scale-up comparison of water washing with activated-carbon adsorption using HYSYS and Aspen Process Economic Analyzer. The models show that for plants processing less than 500 m 3 /h water scrubbing was cost effective compared with activated carbon. However, against current fossil natural-gas cost of about 1 p/kWh in the UK both relied heavily on governmental subsidies to become economically feasible. For plants operating at 1000 m 3 /hr, the treatment costs were reduced to below 1.5 p/kWh for water scrubbing and 0.9 p/kWh for activated carbon where the main benefits of activated carbon were lower capital and operating costs and virtually no water losses. It is envisioned that this method can significantly aid the production of sustainable bio-methane. Copyright © 2017 Elsevier Ltd. All rights reserved.
Chen, Peng; Wang, Zhuyuan; Zong, Shenfei; Chen, Hui; Zhu, Dan; Zhong, Yuan; Cui, Yiping
2014-10-01
p-Aminothiophenol (pATP) functionalized multi-walled carbon nanotubes (MWCNTs) have been demonstrated as an efficient pH sensor for living cells. The proposed sensor employs gold/silver core-shell nanoparticles (Au@Ag NPs) functionalized MWCNTs hybrid structure as the surface-enhanced Raman scattering (SERS) substrate and pATP molecules as the SERS reporters, which possess a pH-dependent SERS performance. By using MWCNTs as the substrate to be in a state of aggregation, the pH sensing range could be extended to pH 3.0∼14.0, which is much wider than that using unaggregated Au@Ag NPs without MWCNTs. Furthermore, the pH-sensitive performance was well retained in living cells with a low cytotoxicity. The developed SERS-active MWCNTs-based nanocomposite is expected to be an efficient intracellular pH sensor for bio-applications.
Feng, Youjun; Kumar, Ritesh; Ravcheev, Dmitry A; Zhang, Huimin
2015-08-01
Recently, we determined that BioR, the GntR family of transcription factor, acts as a repressor for biotin metabolism exclusively distributed in certain species of α-proteobacteria, including the zoonotic agent Brucella melitensis and the plant pathogen Agrobacterium tumefaciens. However, the scenario is unusual in Paracoccus denitrificans, another closely related member of the same phylum α-proteobacteria featuring with denitrification. Not only does it encode two BioR homologs Pden_1431 and Pden_2922 (designated as BioR1 and BioR2, respectively), but also has six predictive BioR-recognizable sites (the two bioR homolog each has one site, whereas the two bio operons (bioBFDAGC and bioYB) each contains two tandem BioR boxes). It raised the possibility that unexpected complexity is present in BioR-mediated biotin regulation. Here we report that this is the case. The identity of the purified BioR proteins (BioR1 and BioR2) was confirmed with LC-QToF-MS. Phylogenetic analyses combined with GC percentage raised a possibility that the bioR2 gene might be acquired by horizontal gene transfer. Gel shift assays revealed that the predicted BioR-binding sites are functional for the two BioR homologs, in much similarity to the scenario seen with the BioR site of A. tumefaciens bioBFDAZ. Using the A. tumefaciens reporter system carrying a plasmid-borne LacZ fusion, we revealed that the two homologs of P. denitrificans BioR are functional repressors for biotin metabolism. As anticipated, not only does the addition of exogenous biotin stimulate efficiently the expression of bioYB operon encoding biotin transport/uptake system BioY, but also inhibits the transcription of the bioBFDAGC operon resembling the de novo biotin synthetic pathway. EMSA-based screening failed to demonstrate that the biotin-related metabolite is involved in BioR-DNA interplay, which is consistent with our former observation with Brucella BioR. Our finding defined a complex regulatory network for biotin
Feng, Youjun; Kumar, Ritesh; Ravcheev, Dmitry A; Zhang, Huimin
2015-01-01
Recently, we determined that BioR, the GntR family of transcription factor, acts as a repressor for biotin metabolism exclusively distributed in certain species of α-proteobacteria, including the zoonotic agent Brucella melitensis and the plant pathogen Agrobacterium tumefaciens. However, the scenario is unusual in Paracoccus denitrificans, another closely related member of the same phylum α-proteobacteria featuring with denitrification. Not only does it encode two BioR homologs Pden_1431 and Pden_2922 (designated as BioR1 and BioR2, respectively), but also has six predictive BioR-recognizable sites (the two bioR homolog each has one site, whereas the two bio operons (bioBFDAGC and bioYB) each contains two tandem BioR boxes). It raised the possibility that unexpected complexity is present in BioR-mediated biotin regulation. Here we report that this is the case. The identity of the purified BioR proteins (BioR1 and BioR2) was confirmed with LC-QToF-MS. Phylogenetic analyses combined with GC percentage raised a possibility that the bioR2 gene might be acquired by horizontal gene transfer. Gel shift assays revealed that the predicted BioR-binding sites are functional for the two BioR homologs, in much similarity to the scenario seen with the BioR site of A. tumefaciens bioBFDAZ. Using the A. tumefaciens reporter system carrying a plasmid-borne LacZ fusion, we revealed that the two homologs of P. denitrificans BioR are functional repressors for biotin metabolism. As anticipated, not only does the addition of exogenous biotin stimulate efficiently the expression of bioYB operon encoding biotin transport/uptake system BioY, but also inhibits the transcription of the bioBFDAGC operon resembling the de novo biotin synthetic pathway. EMSA-based screening failed to demonstrate that the biotin-related metabolite is involved in BioR-DNA interplay, which is consistent with our former observation with Brucella BioR. Our finding defined a complex regulatory network for biotin
Cao, Dan; Zong, Liang-gang; Xiao, Jun; Zhang, Qian; Zhao, Yan
2010-10-01
Field trials of organic farming were conducted to examine the effects of different bio-fertilizers on the organically cultured cucumber growth, soil enzyme activities, and soil microbial biomass. Four treatments were installed, i. e., organic fertilizer only (CK), bio-fertilizer "Zhonghe" combined with organic fertilizer (ZHH), bio-fertilizer "NST" combined with organic fertilizer (NST), and bio-fertilizer "Bio" combined with organic fertilizer (BIO). Bio-fertilizers combined with organic fertilizer increased the cucumber yield significantly, and improved the root growth and leaf chlorophyll content. Comparing with that in CK, the cucumber yield in treatments ZHH, NST, and BIO was increased by 10.4%, 12.4%, and 29.2%, respectively. At the seedling stage, early flowering stage, and picking time of cucumber, the soil microbial biomass C and N in treatments ZHH, NST, and BIO were significantly higher than that in CK, and the activities of soil urease, acid phosphatase, and catalase were also higher.
Novel method of dual fiber Bragg gratings integrated in fiber ring laser for biochemical sensors
NASA Astrophysics Data System (ADS)
Bui, H.; Pham, T. B.; Nguyen, V. A.; Pham, V. D.; Do, T. C.; Nguyen, T. V.; Hoang, T. H. C.; Le, H. T.; Pham, V. H.
2018-05-01
Optical sensors have been shown to be very effective for measuring the toxic content in liquid and air environments. Optical sensors, which operate based on the wavelength shift of the optical signals, require an expensive spectrometer. In this paper, we propose a new configuration of the optical sensor device for measuring wavelength shift without using a spectrometer. This configuration has a large potential for application in biochemical sensing techniques, and comes with a low cost. This configuration uses dual fiber Bragg gratings (FBGs) integrated in a fiber ring laser structure of erbium-doped fiber, in which one FBG is used as a reference to sweep over the applicable spectrum of the etched-Bragg grating. The etched-FBG as a sensing probe is suitable for bio- and/or chemical sensors. The sensitivity and accuracy of the sensor system can be improved by the narrow linewidth of emission spectra from the laser, the best limit of detection of this sensor is 1.5 × 10‑4 RIU (RIU: refractive index unit), as achieved by the optical sensor using a high resolution spectrometer. This sensor system has been experimentally investigated to detect different types of organic compounds, gasoline, mixing ratios of organic solvents in gasoline, and nitrate concentration in water samples. The experimental results show that this sensing method could determine different mixing ratios of organic solvents with good repeatability, high accuracy, and rapid response: e.g. for ethanol and/or methanol in gasoline RON 92 (RON: research octane number) of 0%–14% v/v, and nitrate in water samples at a low concentration range of 0–50 ppm. These results suggest that the proposed configuration can construct low-cost and accurate biochemical sensors.
BioPCD - A Language for GUI Development Requiring a Minimal Skill Set.
Alvare, Graham Gm; Roche-Lima, Abiel; Fristensky, Brian
2012-11-01
BioPCD is a new language whose purpose is to simplify the creation of Graphical User Interfaces (GUIs) by biologists with minimal programming skills. The first step in developing BioPCD was to create a minimal superset of the language referred to as PCD (Pythonesque Command Description). PCD defines the core of terminals and high-level nonterminals required to describe data of almost any type. BioPCD adds to PCD the constructs necessary to describe GUI components and the syntax for executing system commands. BioPCD is implemented using JavaCC to convert the grammar into code. BioPCD is designed to be terse and readable and simple enough to be learned by copying and modifying existing BioPCD files. We demonstrate that BioPCD can easily be used to generate GUIs for existing command line programs. Although BioPCD was designed to make it easier to run bioinformatics programs, it could be used in any domain in which many useful command line programs exist that do not have GUI interfaces.
BioPCD - A Language for GUI Development Requiring a Minimal Skill Set
Alvare, Graham GM; Roche-Lima, Abiel; Fristensky, Brian
2016-01-01
BioPCD is a new language whose purpose is to simplify the creation of Graphical User Interfaces (GUIs) by biologists with minimal programming skills. The first step in developing BioPCD was to create a minimal superset of the language referred to as PCD (Pythonesque Command Description). PCD defines the core of terminals and high-level nonterminals required to describe data of almost any type. BioPCD adds to PCD the constructs necessary to describe GUI components and the syntax for executing system commands. BioPCD is implemented using JavaCC to convert the grammar into code. BioPCD is designed to be terse and readable and simple enough to be learned by copying and modifying existing BioPCD files. We demonstrate that BioPCD can easily be used to generate GUIs for existing command line programs. Although BioPCD was designed to make it easier to run bioinformatics programs, it could be used in any domain in which many useful command line programs exist that do not have GUI interfaces. PMID:27818582
Microbial conversion of biomass into bio-based polymers.
Kawaguchi, Hideo; Ogino, Chiaki; Kondo, Akihiko
2017-12-01
The worldwide market for plastics is rapidly growing, and plastics polymers are typically produced from petroleum-based chemicals. The overdependence on petroleum-based chemicals for polymer production raises economic and environmental sustainability concerns. Recent progress in metabolic engineering has expanded fermentation products from existing aliphatic acids or alcohols to include aromatic compounds. This diversity provides an opportunity to expand the development and industrial uses of high-performance bio-based polymers. However, most of the biomonomers are produced from edible sugars or starches that compete directly with food and feed uses. The present review focuses on recent progress in the microbial conversion of biomass into bio-based polymers, in which fermentative products from renewable feedstocks serve as biomonomers for the synthesis of bio-based polymers. In particular, the production of biomonomers from inedible lignocellulosic feedstocks by metabolically engineered microorganisms and the synthesis of bio-based engineered plastics from the biological resources are discussed. Copyright © 2017 Elsevier Ltd. All rights reserved.
In the trail of a new bio-sensor for measuring strain in bone: osteoblastic biocompatibility.
Carvalho, Lídia; Alberto, Nélia J; Gomes, Pedro S; Nogueira, Rogério N; Pinto, João L; Fernandes, Maria H
2011-06-15
Fibre Bragg Grating (FBG) is an optical sensor recorded within the core of a standard optical fibre, which responds faithfully to strain and temperature. FBG sensors are a promising alternative to other sensing methodologies to assess bone mechanics in vivo. However, response of bone cells/bone tissue to FBGs and its sensing capability in this environment have not been recorded yet. The present study addressed these issues in long-term human osteoblastic cell cultures. Results showed that osteoblastic cells were able to adhere and proliferate over the fibre and, also, the protective polymer coating. RT-PCR analysis showed the expression of Col I, ALP, BMP-2, M-CSF, RANKL and OPG. In addition, cultures presented high ALP activity and the formation of a calcium phosphate mineralized extracellular matrix. Cell behavior over the fibre without and with the coating polymer was similar to that found in cultures grown in standard tissue culture plates (control). In addition to the excellent osteoblastic cytocompatibility, FBGs maintained the physical integrity and functionality, as its sensing capability was not affected through the culture period. Results suggest the possibility of in vivo osseointegration of the optical fibre/FBGs anticipating a variety of applications in bone mechanical dynamics. Copyright © 2011 Elsevier B.V. All rights reserved.
Kinetic Modeling using BioPAX ontology
Ruebenacker, Oliver; Moraru, Ion. I.; Schaff, James C.; Blinov, Michael L.
2010-01-01
Thousands of biochemical interactions are available for download from curated databases such as Reactome, Pathway Interaction Database and other sources in the Biological Pathways Exchange (BioPAX) format. However, the BioPAX ontology does not encode the necessary information for kinetic modeling and simulation. The current standard for kinetic modeling is the System Biology Markup Language (SBML), but only a small number of models are available in SBML format in public repositories. Additionally, reusing and merging SBML models presents a significant challenge, because often each element has a value only in the context of the given model, and information encoding biological meaning is absent. We describe a software system that enables a variety of operations facilitating the use of BioPAX data to create kinetic models that can be visualized, edited, and simulated using the Virtual Cell (VCell), including improved conversion to SBML (for use with other simulation tools that support this format). PMID:20862270
Rubinstein, Yaffa R; Posada de la Paz, Manuel; Mora, Marina
2017-01-01
Well-annotated and properly preserved specimens are crucial both for diagnostic purposes and for use in basic and pre-clinical research, and are especially important for rare disease (RD) studies. Several consortia have been established in the recent years in order to facilitate research and to maximise access to rare biological samples and data stored in rare disease biobanks and registries, among them the EuroBioBank network and the Spain National Rare Disease Registry (RDR) and Biobank (BioNER).EuroBioBank, established in 2001, was the first network of RD biobanks to operate in Europe as a service distributing human DNA, cells, and tissue to the scientific community conducting research on rare diseases.The Spanish RDR and BioNER were created for facilitating rare disease research and health-related matters. The coordination of these two bodies represents an example of great scientific value as biological samples donated by patients at BioNER are linked to clinical information collected in the RDR.Rare disease biobanks and registries will need for the future to increase their effort to improve interconnection so to enable investigators to better locate samples and associated data, while protecting security of the data and privacy of the participants and adhering to international ethical and legal requirements.
Label-free optical resonant sensors for biochemical applications
NASA Astrophysics Data System (ADS)
Ciminelli, Caterina; Campanella, Clarissa Martina; Dell'Olio, Francesco; Campanella, Carlo Edoardo; Armenise, Mario Nicola
2013-03-01
For a number of years, the scientific community has been paying growing attention to the monitoring and enhancement of public health and the quality of life through the detection of all dangerous agents for the human body, including gases, proteins, virus, and bacterial agents. When these agents are detected through label-free biochemical sensors, the molecules are not modified structurally or functionally by adding fluorescent or radioactive dyes. This work focuses on label-free optical ring resonator-based configurations suited for bio-chemical sensing, highlighting their physical aspects and specific applications. Resonant wavelength shift and the modal splitting occurring when the analyte interacts with microresonant structures are the two major physical aspects analyzed in this paper. Competitive optical platforms proposed in the literature are also illustrated together with their properties and performance.
Mastering multi-depth bio-chip patterns with DVD LBRs
NASA Astrophysics Data System (ADS)
Carson, Doug
2017-08-01
Bio chip and bio disc are rapidly growing technologies used in medical, health and other industries. While there are numerous unique designs and features, these products all rely on precise three-dimensional micro-fluidic channels or arrays to move, separate and combine samples under test. These bio chip and bio disc consumables are typically manufactured by molding these parts to a precise three-dimensional pattern on a negative metal stamper, or they can be made in smaller quantities using an appropriate curable resin and a negative mold/stamper. Stampers required for bio chips have been traditionally made using either micro machining or XY stepping lithography. Both of these technologies have their advantages as well as limitations when it comes to creating micro-fluidic patterns. Significant breakthroughs in continuous maskless lithography have enabled accurate and efficient manufacturing of micro-fluidic masters using LBRs (Laser Beam Recorders) and DRIE (Deep Reactive Ion Etching). The important advantages of LBR continuous lithography vs. XY stepping lithography and micro machining are speed and cost. LBR based continuous lithography is >100x faster than XY stepping lithography and more accurate than micro machining. Several innovations were required in order to create multi-depth patterns with sub micron accuracy. By combining proven industrial LBRs with DCA's G3-VIA pattern generator and DRIE, three-dimensional bio chip masters and stampers are being manufactured efficiently and accurately.
Palladium catalyzed hydrogenation of bio-oils and organic compounds
Elliott, Douglas C [Kennewick, WA; Hu, Jianli [Richland, WA; Hart,; Todd, R [Kennewick, WA; Neuenschwander, Gary G [Burbank, WA
2011-06-07
The invention provides palladium-catalyzed hydrogenations of bio-oils and certain organic compounds. Experimental results have shown unexpected and superior results for palladium-catalyzed hydrogenations of organic compounds typically found in bio-oils.
Palladium catalyzed hydrogenation of bio-oils and organic compounds
Elliott, Douglas C [Richland, WA; Hu, Jianli [Kennewick, WA; Hart, Todd R [Kennewick, WA; Neuenschwander, Gary G [Burbank, WA
2008-09-16
The invention provides palladium-catalyzed hydrogenations of bio-oils and certain organic compounds. Experimental results have shown unexpected and superior results for palladium-catalyzed hydrogenations of organic compounds typically found in bio-oils.
The BioHub Knowledge Base: Ontology and Repository for Sustainable Biosourcing.
Read, Warren J; Demetriou, George; Nenadic, Goran; Ruddock, Noel; Stevens, Robert; Winter, Jerry
2016-06-01
The motivation for the BioHub project is to create an Integrated Knowledge Management System (IKMS) that will enable chemists to source ingredients from bio-renewables, rather than from non-sustainable sources such as fossil oil and its derivatives. The BioHubKB is the data repository of the IKMS; it employs Semantic Web technologies, especially OWL, to host data about chemical transformations, bio-renewable feedstocks, co-product streams and their chemical components. Access to this knowledge base is provided to other modules within the IKMS through a set of RESTful web services, driven by SPARQL queries to a Sesame back-end. The BioHubKB re-uses several bio-ontologies and bespoke extensions, primarily for chemical feedstocks and products, to form its knowledge organisation schema. Parts of plants form feedstocks, while various processes generate co-product streams that contain certain chemicals. Both chemicals and transformations are associated with certain qualities, which the BioHubKB also attempts to capture. Of immediate commercial and industrial importance is to estimate the cost of particular sets of chemical transformations (leading to candidate surfactants) performed in sequence, and these costs too are captured. Data are sourced from companies' internal knowledge and document stores, and from the publicly available literature. Both text analytics and manual curation play their part in populating the ontology. We describe the prototype IKMS, the BioHubKB and the services that it supports for the IKMS. The BioHubKB can be found via http://biohub.cs.manchester.ac.uk/ontology/biohub-kb.owl .
Alarfaj, Nawal Ahmad; El-Tohamy, Maha Farouk
2016-01-01
The present article introduces a new approach to fabricate carbon paste sensors, including carbon paste, modified carbon paste inclusion β-cyclodextrin, and carbon nanotubes for the quantification of dorzolamide hydrochloride (DRZ). This study is mainly based on the construction of three different carbon paste sensors by the incorporation of DRZ with phosphotungstic acid (PTA) to form dorzolamide-phosphotungstate (DRZ-PT) as an electroactive material in the presence of the solvent mediator ortho-nitrophenyloctyl ether (o-NPOE). The fabricated conventional carbon paste sensor (sensor I), as well as the other modified carbon paste sensors using β-cyclodextrin (sensor II) and carbon nanotubes (sensor III), have been investigated. The sensors displayed Nernstian responses of 55.4 ± 0.6, 56.4 ± 0.4 and 58.1 ± 0.2 mV·decade−1 over concentration ranges of 1.0 × 10−5–1.0 × 10−2, 1.0 × 10−6–1.0 × 10−2, and 5.0 × 10−8–1.0 × 10−2 mol·L−1 with lower detection limits of 5.0 × 10−6, 5.0 × 10−7, and 2.5 × 10−9 mol·L−1 for sensors I, II, and III, respectively. The critical performance of the developed sensors was checked with respect to the effect of various parameters, including pH, selectivity, response time, linear concentration relationship, lifespan, etc. Method validation was applied according to the international conference on harmonisation of technical requirements for registration of pharmaceuticals for human use ICH guidelines. The developed sensors were employed for the determination of DRZ in its bulk and dosage forms, as well as bio-samples. The observed data were statistically analyzed and compared with those obtained from other published methods. PMID:27918458
Alarfaj, Nawal Ahmad; El-Tohamy, Maha Farouk
2016-12-02
The present article introduces a new approach to fabricate carbon paste sensors, including carbon paste, modified carbon paste inclusion β-cyclodextrin, and carbon nanotubes for the quantification of dorzolamide hydrochloride (DRZ). This study is mainly based on the construction of three different carbon paste sensors by the incorporation of DRZ with phosphotungstic acid (PTA) to form dorzolamide-phosphotungstate (DRZ-PT) as an electroactive material in the presence of the solvent mediator ortho-nitrophenyloctyl ether ( o -NPOE). The fabricated conventional carbon paste sensor (sensor I), as well as the other modified carbon paste sensors using β-cyclodextrin (sensor II) and carbon nanotubes (sensor III), have been investigated. The sensors displayed Nernstian responses of 55.4 ± 0.6, 56.4 ± 0.4 and 58.1 ± 0.2 mV·decade -1 over concentration ranges of 1.0 × 10 -5 -1.0 × 10 -2 , 1.0 × 10 -6 -1.0 × 10 -2 , and 5.0 × 10 -8 -1.0 × 10 -2 mol·L -1 with lower detection limits of 5.0 × 10 -6 , 5.0 × 10 -7 , and 2.5 × 10 -9 mol·L -1 for sensors I, II, and III, respectively. The critical performance of the developed sensors was checked with respect to the effect of various parameters, including pH, selectivity, response time, linear concentration relationship, lifespan, etc. Method validation was applied according to the international conference on harmonisation of technical requirements for registration of pharmaceuticals for human use ICH guidelines. The developed sensors were employed for the determination of DRZ in its bulk and dosage forms, as well as bio-samples. The observed data were statistically analyzed and compared with those obtained from other published methods.
BioCreative Workshops for DOE Genome Sciences: Text Mining for Metagenomics
DOE Office of Scientific and Technical Information (OSTI.GOV)
Wu, Cathy H.; Hirschman, Lynette
The objective of this project was to host BioCreative workshops to define and develop text mining tasks to meet the needs of the Genome Sciences community, focusing on metadata information extraction in metagenomics. Following the successful introduction of metagenomics at the BioCreative IV workshop, members of the metagenomics community and BioCreative communities continued discussion to identify candidate topics for a BioCreative metagenomics track for BioCreative V. Of particular interest was the capture of environmental and isolation source information from text. The outcome was to form a “community of interest” around work on the interactive EXTRACT system, which supported interactive taggingmore » of environmental and species data. This experiment is included in the BioCreative V virtual issue of Database. In addition, there was broad participation by members of the metagenomics community in the panels held at BioCreative V, leading to valuable exchanges between the text mining developers and members of the metagenomics research community. These exchanges are reflected in a number of the overview and perspective pieces also being captured in the BioCreative V virtual issue. Overall, this conversation has exposed the metagenomics researchers to the possibilities of text mining, and educated the text mining developers to the specific needs of the metagenomics community.« less
Mengal, Philippe; Wubbolts, Marcel; Zika, Eleni; Ruiz, Ana; Brigitta, Dieter; Pieniadz, Agata; Black, Sarah
2018-01-25
This article discusses the preparation, structure and objectives of the Bio-based Industries Joint Undertaking (BBI JU). BBI JU is a public-private partnership (PPP) between the European Commission (EC) and the Bio-based Industries Consortium (BIC), the industry-led private not-for-profit organisation representing the private sectors across the bio-based industries. The model of the public-private partnership has been successful as a new approach to supporting research and innovation and de-risking investment in Europe. The BBI JU became a reality in 2014 and represents the largest industrial and economic cooperation endeavour financially ever undertaken in Europe in the area of industrial biotechnologies. It is considered to be one of the most forward-looking initiatives under Horizon 2020 and demonstrates the circular economy in action. The BBI JU will be the catalyst for this strategy to mobilise actors across Europe including large industry, small and medium-sized enterprises (SMEs), all types of research organisations, networks and universities. It will support regions and in doing so, the European Union Member States and associated countries in the implementation of their bioeconomy strategies. Copyright © 2017 Elsevier B.V. All rights reserved.
The water footprint of sweeteners and bio-ethanol.
Gerbens-Leenes, Winnie; Hoekstra, Arjen Y
2012-04-01
An increasing demand for food together with a growing demand for energy crops result in an increasing demand for and competition over water. Sugar cane, sugar beet and maize are not only essential food crops, but also important feedstock for bio-ethanol. Crop growth requires water, a scarce resource. This study aims to assess the green, blue and grey water footprint (WF) of sweeteners and bio-ethanol from sugar cane, sugar beet and maize in the main producing countries. The WFs of sweeteners and bio-ethanol are mainly determined by the crop type that is used as a source and by agricultural practise and agro-climatic conditions; process water footprints are relatively small. The weighted global average WF of sugar cane is 209 m(3)/tonne; for sugar beet this is 133 m(3)/tonne and for maize 1222 m(3)/tonne. Large regional differences in WFs indicate that WFs of crops for sweeteners and bio-ethanol can be improved. It is more favourable to use maize as a feedstock for sweeteners or bio-ethanol than sugar beet or sugar cane. The WF of sugar cane contributes to water stress in the Indus and Ganges basins. In the Ukraine, the large grey WF of sugar beet contributes to water pollution. In some western European countries, blue WFs of sugar beet and maize need a large amount of available blue water for agriculture. The allocation of the limited global water resources to bio-energy on a large scale will be at the cost of water allocation to food and nature. Copyright © 2011 Elsevier Ltd. All rights reserved.
The electromagnetic bio-field: clinical experiments and interferences
Burnei, G; Hodorogea, D; Georgescu, I; Gavriliu, Ş; Drăghici, I; Dan, D; Vlad, C; Drăghici, L
2012-01-01
Introduction: One of the most important factors is the technical and scientifically rapid development that is continually modifying the world we live in and polluting it with electromagnetic radiations. A functional and structural influence of magnetic and electromagnetic field on living organisms is presented in the literature by many performed experiments. Material and methods: The notion of bio-field represents the electromagnetic field generated by the bio-structures, not only in their normal physiological activities but also in their pathological states. There is a tight interdependency between the bio-field and the bio-structure, which respects the primary notion of an electromagnetic field given by the Maxwell-Faraday laws, in which, the electromagnetic phenomena are simplified to the field variations. These variations can be expressed in a coherent differential equation system that bounds the field vectors to different space points at different time moments. Results: The living organisms cannot contain electrostatic and magneto-static fields due to the intense activity of the bio-structures. The biochemical reactions that have high rhythms and speeds always impose the electrodynamics character of the biologic field that also corresponds to the stability of the protein molecule that can be explained only through a dynamic way. The existent energy is not considered an exciting agent, and it does not lead to any effects. Conclusions: The parameters of these elementary bio-fields cannot yet be fully known due to technical reasons. The biological structures are very complex ones and undergo continuous dynamical activity. That is why the calculus model should be related to the constant dynamics, nowadays being very difficult to express. PMID:22802878
BioSPICE: access to the most current computational tools for biologists.
Garvey, Thomas D; Lincoln, Patrick; Pedersen, Charles John; Martin, David; Johnson, Mark
2003-01-01
The goal of the BioSPICE program is to create a framework that provides biologists access to the most current computational tools. At the program midpoint, the BioSPICE member community has produced a software system that comprises contributions from approximately 20 participating laboratories integrated under the BioSPICE Dashboard and a methodology for continued software integration. These contributed software modules are the BioSPICE Dashboard, a graphical environment that combines Open Agent Architecture and NetBeans software technologies in a coherent, biologist-friendly user interface. The current Dashboard permits data sources, models, simulation engines, and output displays provided by different investigators and running on different machines to work together across a distributed, heterogeneous network. Among several other features, the Dashboard enables users to create graphical workflows by configuring and connecting available BioSPICE components. Anticipated future enhancements to BioSPICE include a notebook capability that will permit researchers to browse and compile data to support model building, a biological model repository, and tools to support the development, control, and data reduction of wet-lab experiments. In addition to the BioSPICE software products, a project website supports information exchange and community building.
Bio-nano interactions detected by nanochannel electrophoresis.
Luan, Binquan
2016-08-01
Engineered nanoparticles have been widely used in industry and are present in many consumer products. However, their bio-safeties especially in a long term are largely unknown. Here, a nanochannel-electrophoresis-based method is proposed for detecting the potential bio-nano interactions that may further lead to damages to human health and/or biological environment. Through proof-of-concept molecular dynamics simulations, it was demonstrated that the transport of a protein-nanoparticle complex is very different from that of a protein along. By monitoring the change of ionic currents induced by a transported analyte as well as the transport velocities of the analyte, the complex (with bio-nano interaction) can be clearly distinguished from the protein alone (with no interaction with tested nanoparticles). © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.