Sequence of the radioactive tryptic peptide obtained after inactivating the F1-ATPase of the thermophilic bacterium PS3 with 5'-p-fluorosulfonylbenzoyl(3H)adenosine at 65 degrees C

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bullough, D.A.; Yoshida, M.; Allison, W.S.

1986-02-01

Following a lag of about 30 min, the F1-ATPase from the thermophilic bacterium, PS3 (TF1), was inactivated slowly by 0.8 mM 5'-p-fluorosulfonylbenzoyladenosine (FSBA) at 23 degrees C and pH 7.0. When the enzyme was treated with 0.2 mM FSBA at pH 7.0 and 23 degrees C for 15 min and gel-filtered, no enzyme activity was lost. However, the lag in inactivation was abolished when the enzyme was subsequently incubated with 2.0 mM FSBA at 23 degrees C in the pH range from 6.8 to 10.0. The pH-inactivation profile obtained under these conditions revealed a pK alpha of about 9.3 whichmore » was associated with the inactivation. When pretreated TF1 was inactivated at 23 degrees C with (3H)FSBA by about 90%, greater than 20 mol of (3H)SBA was incorporated per mole of enzyme. TF1 was inactivated rapidly by 0.8 mM FSBA at pH 6.4 and 65 degrees C, and no lag was observed. Following inactivation of TF1 with 0.8 mM (3H)FSBA at 65 degrees C and pH 6.4, about 10 mol of (3H)SBA was incorporated per mole of enzyme. When a tryptic digest of the labeled enzyme was fractionated by reversed-phase high-performance liquid chromatography, a single major radioactive peptide was isolated. When subjected to automatic Edman degradation, this peptide was shown to have the amino acid sequence: A-L-A-P-E-I-V-G-E-E-H-X-Q-V-A-R, where X indicates that a phenylthiohydantoin derivative was not detected in cycle 12. However, from the DNA sequence of the gene encoding the subunit of TF1 (Y. Kagawa, M. Ishizuka, T. Saishu, and S. Nakao (1985)), this position has been shown to be occupied by tyrosine. This tyrosine is homologous with beta-Tyr-368 of the bovine mitochondrial F1-ATPase (MF1) the modification of which is responsible for the inactivation MF1 by FSBA.« less

Geothermal Frontier: Penetrate a boundary between hydrothermal convection and heat conduction zones to create 'Beyond Brittle Geothermal Reservoir'

NASA Astrophysics Data System (ADS)

Tsuchiya, N.; Asanuma, H.; Sakaguchi, K.; Okamoto, A.; Hirano, N.; Watanabe, N.; Kizaki, A.

2013-12-01

experiments, our research goals are 1)Analysis and understanding of geothermal structure and geofluids in ductile condition of the Japanese Island arc, 2)Fundamental technologies of drilling under ductile region for geothermal reservoir, 3) Development of geothermal reservoir simulator of two phase and multiphase flow including supercritical state through rock fracture, 4) Lab scale support for ICDP-JBBP, 5) Application of new EGS technologies to conventional geothermal fields as recovery from the 2011 Great East Japan Earthquake and energy crisis in Japan. [Publications Relevant to the Research] Tsuchiya, N. and Hirano, N. (2007), ISLAND ARC, 16, 6-15. Okamoto, A., Saishu, H., Hirano, N. & Tsuchiya, N. (2010) Geochimica et Cosmochimica Acta, 74, 3692-3706. Majer, E.L., Baria, R., Stark, M., Oates, S., Bonner, J. Smith, B. & Asanuma H., (2007) Geothermics, 36, 185-222. Watanabe, N., Hirano, N. Tsuchiya, N. (2009) Journal of Geophysical Research B: Solid Earth, 114(4), B04208.

A brief history of recognition of the Kuroshio

NASA Astrophysics Data System (ADS)

Kawai, Hideo

1998-10-01

Since ancient times the Japanese and Asians have encountered the Kuroshio. For instance, records of long-distance driftings that must have been greatly influenced by the Kuroshio are found in official chronicles of Japan from before 833 and a biography of Ganjin, the Chinese Buddhist who arrived in Japan on a religious mission in 754 across the Kuroshio. In the seventh and eighth centuries, some of the ships of Japanese envoys to China during the Tang Dynasty crossed the Kuroshio in the East China Sea, despite frequent incidents of ship wrecks. In the Momoyama and early Edo periods (1592-1635) Japanese ships with the shogunate license for foreign trade crossed the Kuroshio east of Taiwan bound for Luzon. During those periods, however, no mention of the Kuroshio has been found, except for that of S. Vizcaíno, the Spanish navigator who reported a segment of strong current east of the Boso Peninsula in 1614. After the National Isolation Policy was enforced in 1635, the building of large ships and navigation in waters beyond where the coast was visible were prohibited in Japan, and hence it seems unlikely that many early books from this period will mention the Kuroshio. However, by searching and deciphering early books, the author has found many important notes, descriptions and illustrations referring to the Kuroshio in maps and books from the Edo period (1603-1867). Terms “Luo-Ji” and “Wei-Lü”, Chinese equivalents of “Kuroshio”, were searched for in Chinese classics and were examined. The term “Luo-Ji” (Rakusai in Japanese) was cited by some Japanese books written in the Edo period referring to the Kuroshio in the East China Sea. Meanwhile, the term “Wei-Lü” (Biryo in Japanese) was considered to be too mythical to be used in Japan as indicating the Kuroshio, although Needham (1971) inferred that the “Wei-Lü” had been used since ancient times as the Chinese equivalent of “Kuroshio”. To clarify the evolution of types of image of the