Science.gov

Sample records for konsa mirja ots

  1. Master: 3 OT

    NASA Astrophysics Data System (ADS)

    Balanutsa, P.; Lipunov, V.; Buckley, D.; Tlatov, A.; Gorbovskoy, E.; Tyurina, N.; Kuznetsov, A.; Kornilov, V.; Kuvshinov, D.; Popova, E.; Vlasenko, D.; Shumkov, V.; Potter, S.; Kniazev, A.; Budnev, N.; Gress, O.; Ivanov, K.; Senik, V.; Dormidontov, D.; Parhomenko, A. V.

    2016-08-01

    MASTER-Kislovodsk auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 21h 33m 50.58s +06d 51m 22.5s on 2016-07-27.94690 UT. The OT unfiltered magnitude is 17.8m (limit 18.0m).

  2. MASTER: QSO flare and OTs

    NASA Astrophysics Data System (ADS)

    Gress, O.; Shumkov, V.; Pogrosheva, T.; Lipunov, V.; Rebolo, R.; Serra-Ricart, M.; Podesta, R.; Levato, H.; Gorbovskoy, E.; Tiurina, N.; Balanutsa, P.; Kuznetsov, A.; Kornilov, V.; Ivanov, K.; Vladimirov, V.; Lopez, C.; Podesta, F.; Saffe, C.

    2016-10-01

    MASTER-OAFA auto-detection system (Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 01h 50m 52.97s -45d 10m 15.9s on 2016-10-06.21896 UT with unfiltered m_OT=17.4m (limit 20.6m).

  3. MASTER: PSN in PGC135878 and OTs

    NASA Astrophysics Data System (ADS)

    Balanutsa, P.; Shumkov, V.; Lipunov, V.; Buckley, D.; Lopez, R. Rebolo; Gorbovskoy, E.; Tiurina, N.; Kuznetsov, A.; Kornilov, V.; Gorbunov, I.; Gress, O.; Ricart, M. Serra; Israelian, G.; Potter, S.; Kniazev, A.

    2016-06-01

    MASTER-IAC auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 349171 ) discovered OT source at (RA, Dec) = 18h 02m 41.87s +09d 03m 12.9s on 2016-06-05.16160 UT. The OT unfiltered magnitude is (limit 19.1m).The OT is seen in 10 images.

  4. Two novel splice variants of SOX2OT, SOX2OT-S1, and SOX2OT-S2 are coupregulated with SOX2 and OCT4 in esophageal squamous cell carcinoma.

    PubMed

    Shahryari, Alireza; Rafiee, Mahmoud Reza; Fouani, Youssef; Oliae, Nasrin Alipour; Samaei, Nader Mansour; Shafiee, Mohammad; Semnani, Shahryar; Vasei, Mohammad; Mowla, Seyed Javad

    2014-01-01

    Long noncoding RNAs (lncRNAs) have emerged as new regulators of stem cell pluripotency and tumorigenesis. The SOX2 gene, a master regulator of pluripotency, is embedded within the third intron of a lncRNA known as SOX2 overlapping transcript (SOX2OT). SOX2OT has been suspected to participate in regulation of SOX2 expression and/or other related processes; nevertheless, its potential involvement in tumor initiation and/or progression is unclear. Here, we have evaluated a possible correlation between expression patterns of SOX2OT and those of master regulators of pluripotency, SOX2 and OCT4, in esophageal squamous cell carcinoma (ESCC) tissue samples. We have also examined its potential function in the human embryonic carcinoma stem cell line, NTERA2 (NT2), which highly expresses SOX2OT, SOX2, and OCT4. Our data revealed a significant coupregulation of SOX2OT along with SOX2 and OCT4 in tumor samples, compared to the non-tumor tissues obtained from the margin of same tumors. We also identified two novel splice variants of SOX2OT (SOX2OT-S1 and SOX2OT-S2) which coupregulated with SOX2 and OCT4 in ESCCs. Suppressing SOX2OT variants caused a profound alteration in cell cycle distribution, including a 5.9 and 6.9 time increase in sub-G1 phase of cell cycle for SOX2OT-S1 and SOX2OT-S2, respectively. The expression of all variants was significantly diminished, upon the induction of neural differentiation in NT2 cells, suggesting their potential functional links to the undifferentiated state of the cells. Our data suggest a part for SOX2OT spliced variants in tumor initiation and/or progression as well as regulating pluripotent state of stem cells.

  5. MASTER-SAAO: short OT detection

    NASA Astrophysics Data System (ADS)

    Pogrosheva, T.; Lipunov, V.; Chazov, V.; Buckley, D.; Gorbovskoy, E.; Tiurina, N.; Kuznetsov, A.; Balanutsa, P.; Kornilov, V.; Gress, O.; Potter, S.

    2016-09-01

    MASTER-SAAO auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 00h 45m 26.42s -88d 32m 00.3s on 2016-09-23.96355 UT. The OT unfiltered m=18.7m (mlim=20.8m).

  6. MASTER: 2 PSNe and 3 OTs

    NASA Astrophysics Data System (ADS)

    Pogrosheva, T.; Gress, O.; Balanutsa, P.; Lipunov, V.; Buckley, D.; Rebolo Lopez, R.; Serra Ricart, M.; Podesta, R.; Levato, H.; Tiurina, N.; Gorbovskoy, E.; Kuznetsov, A.; Kornilov, V.; Shumkov, V.; Lodieu, N.; Saffe, C.; Lopez, C.; Podesta, F.; Rabinovich, J.; Ivanov, K.; Budnev, N.

    2016-09-01

    MASTER-SAAO auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 05h 42m 31.15s -26d 25m 33.9s on 2016-09-20.06426 UT. The OT unfiltered m=17.3m (mlim=18.7m).

  7. MASTER-OAFA discovery: new bright OT

    NASA Astrophysics Data System (ADS)

    Shumkov, V.; Balanutsa, P.; Pogrosheva, T.; Lipunov, V.; Podesta, R.; Levato, H.; Buckley, D.; Gorbovskoy, E.; Tiurina, N.; Kuznetsov, A.; Kornilov, V.; Chazov, V.; Vlasenko, D.; Vladimirov, V.; Gress, O.; Ivanov, K.; Lopez, C.; Podesta, F.; Saffe, C.; Potter, S.

    2016-10-01

    MASTER-OAFA auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 18h 06m 06.62s -62d 13m 31.3s on 2016-10-19.03627 UT. The OT unfiltered (limit 19.1m).

  8. MASTER-SAAO: bright outbursting OT

    NASA Astrophysics Data System (ADS)

    Shumkov, V.; Lipunov, V.; Buckley, D.; Kornilov, V.; Gorbovskoy, E.; Tiurina, N.; Balanutsa, P.; Gress, O.; Kuznetsov, A.; Kuvshinov, D.; Vladimirov, V.; Gorbunov, I.; Vlasenko, D.; Popova, E.; Potter, S.; Kniazev, A.; Shurpakov, S.

    2016-04-01

    MASTER-SAAO auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 349171 ) discovered OT source at (RA, Dec) = 21h 29m 20.71s -62d 08m 52.2s on 2016-04-27.985 UT. The OT unfiltered magnitude is 17.3m (the limit is 19.3m).

  9. Master-Iac Master-Saao OTs

    NASA Astrophysics Data System (ADS)

    Pogrosheva, T.; Vladimirov, V.; Lipunov, V.; Lopez, R. Rebolo; Buckley, D.; Gorbovskoy, E.; Tiurina, N.; Kuznetsov, A.; Balanutsa, P.; Kornilov, V.; Gorbunov, I.; Gress, O.; Shumkov, V.; Ricart, M. Serra; Israelian, G.; Potter, S.; Kniazev, A.

    2016-06-01

    MASTER-IAC auto-detection system( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 20h 58m 16.98s +23d 58m 12.6s on 2016-06-09.03890 UT. The OT unfiltered magnitude is 17.5m (limit 19.4m).

  10. MASTER: 2 OT discovered in Argentina

    NASA Astrophysics Data System (ADS)

    Shumkov, V.; Pogrosheva, T.; Lipunov, V.; Podesta, R.; Levato, H.; Gorbovskoy, E.; Tiurina, N.; Balanutsa, P.; Kuznetsov, A.; Vladimirov, V.; Gress, O.; Ivanov, K.; Chazov, V.; Lopez, C.; Podesta, F.; Saffe, C.

    2016-10-01

    MASTER-OAFA, located in Argentina, with auto-detection system (Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L) discovered OT source at (RA, Dec) = 03h 19m 42.92s -45d 30m 13.9s on 2016-10-27.27597 UT. The OT unfiltered magnitude is 16.9m (mlim=20.8m).

  11. MASTER: PSN in PGC858421 and OTs

    NASA Astrophysics Data System (ADS)

    Tiurina, N.; Balanutsa, P.; Lipunov, E. Popova V.; Buckley, D.; Gorbovskoy, E.; Kuznetsov, A.; Kornilov, V.; Vlasenko, D.; Gress, O.; Shurpakov, S.; Potter, S.; Kniazev, A.

    2016-07-01

    MASTER-SAAO auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 14h 47m 32.66s -19d 12m 42.1s on 2016-07-09.87385 UT. PSN unfiltered magnitude is (limit 19.8m).

  12. MASTER OT 015347+303844 discovery

    NASA Astrophysics Data System (ADS)

    Balanutsa, P.; Gorbovskoy, E.; Lipunov, V.; Kornilov, V.; Belinski, A.; Shatskiy, N.; Tyurina, N.; Kuvshinov, D.; Chazov, V.; Kuznetsov, A.; Zimnukhov, D.; Kornilov, M.; Tlatov, A.; Parhomenko, A. V.; Dormidontov, D.; Yurkov, V.; Sergienko, Yu.; Varda, D.; Krushinski, V.; Zalozhnih, I.; Kopytova, T.; Popov, A.; Ivanov, K.; Yazev, S.; Budnev, N.; Konstantinov, E.; Lenok, V.; Chuvalaev, O.; Poleschuk, V.; Gres, O.

    2010-10-01

    MASTER auto-detection system at Kislovodsk detected a new transient source at the position (R.A., Dec) = 01h 53m 47.24s , +30d 38m 44s.0 (J2000) with a typical uncertainty of 0.2" at 2010-10-01 23:25:48 (UT). The V magnitude is about 17.9 +-0.1 ( exposition 180s, m_lim = 19.0 ). The OT is seen at next 7 images up 2010-10-12 in R and V. OT is blue: R-V=1.43 at 2010-10-01.9 . There is no minor planet and any object brighter 21 mag in DSS at this place.

  13. MASTER discoveries: dwarf novae, PSN, OT in Andromeda direction

    NASA Astrophysics Data System (ADS)

    Shumkov, V.; Gress, O.; Balanutsa, P.; Lipunov, V.; Rebolo Lopez, R.; Serra Ricart, M.; Gorbovskoy, E.; Tiurina, N.; Kuznetsov, A.; Kornilov, V.; Ivanov, K.

    2016-09-01

    MASTER-IAC auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 00h 41m 26.22s +41d 43m 50.0s on 2016-09-02.12339 UT. The OT unfiltered magnitude is 19.7m, mlim=20.0 The OT is seen in 14 images.

  14. MASTER-IAC: PSN in PGC1671957 and bright OT

    NASA Astrophysics Data System (ADS)

    Balanutsa, P.; Lipunov, V.; Rebolo Lopez, R.; Serra Ricart, M.; Gorbovskoy, E.; Tiurina, N.; Kuznetsov, A.; Kornilov, V.; Gress, O.; Ivanov, K.; Shumkov, V.

    2016-09-01

    MASTER-IAC auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 23h 36m 08.12s +22d 32m 33.1s on 2016-09-07.05299 UT. The OT unfiltered magnitude is 17.8m (limit 20.1m).

  15. MASTER-IAC: high amplitude (>8m) bright OT

    NASA Astrophysics Data System (ADS)

    Lipunov, V.; Lopez, R. Rebolo; Ricart, M. Serra; Tiurina, N.; Gorbovskoy, E.; Balanutsa, P.; Kuznetsov, A.; Kornilov, V.; Gress, O.; Ivanov, K.; Shumkov, V.; Balanutsa, P.; Pogrosheva, T.; Rabinovich, J.; Vlasenko, D.; Shurpakov, S.; Kuvshinov, D.

    2016-09-01

    MASTER-IAC auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 03h 51m 09.53s +15d 28m 56.9s on UT. The OT unfiltered magnitude is (mlim=18.6m).

  16. MASTER: very bright OT in Large Magellanic cloud direction

    NASA Astrophysics Data System (ADS)

    Gorbovskoy, E.; Lipunov, V.; Buckley, D.; Tiurina, N.; Balanutsa, P.; Kornilov, V.; Gorbunov, I.; Kuznetsov, A.; Gress, O.; Vladimirov, V.; Popova, E.; Vlasenko, D.; Kuvshinov, D.; Potter, S.

    2016-05-01

    MASTER-SAAO auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 349171 ) discovered OT source at (RA, Dec) = 05h 10m 32.58s -69d 21m 30.4s on 2016-05-10.72797 UT. The OT unfiltered magnitude is (limit 17.6m).

  17. 75 FR 51169 - OTS Minority Depository Institutions Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-18

    ... Office of Thrift Supervision OTS Minority Depository Institutions Advisory Committee AGENCY: Department... Minority Depository Institutions Advisory Committee will renew for a two-year period beginning August 2...), and with the approval of the Secretary of the Treasury to announce the renewal of the OTS...

  18. 12 CFR 500.10 - The OTS or The Office.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 12 Banks and Banking 6 2014-01-01 2012-01-01 true The OTS or The Office. 500.10 Section 500.10 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY AGENCY ORGANIZATION AND FUNCTIONS General Organization § 500.10 The OTS or The Office. The Office of Thrift Supervision (referred...

  19. MASTER-IAC: bright QSO flare and OT discovery

    NASA Astrophysics Data System (ADS)

    Shumkov, V.; Popova, E.; Gress, O.; Lipunov, V.; Lopez, R. Rebolo; Serra-Ricart, M.; Gorbovskoy, E.; Tiurina, N.; Balanutsa, P.; Kornilov, V.; Kuznetsov, A.; Kuvshinov, D.; Vlasenko, D.; Chazov, V.; Israelyan, G.; Lodieu, N.; Budnev, N.; Ivanov, K.; Shurpakov, S.

    2016-07-01

    MASTER-IAC auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L )discovered OT source at (RA, Dec) = 19h 18m 40.69s +42d 21m 03.3s on 2016-07-25.00863 UT. The OT magnitude in unfiltered is 17.9m (limit 18.7m).

  20. Master-Iac Master-Saao 2 bright OTs

    NASA Astrophysics Data System (ADS)

    Gress, O.; Pogrosheva, T.; Lipunov, V.; Lopez, R. Rebolo; Buckley, D.; Gorbovskoy, E.; Tiurina, N.; Balanutsa, P.; Gorbunov, I.; Kuznetsov, A.; Popova, E.; Kuvshinov, D.; Serra-Ricart, M.; Israelian, G.; Potter, S.; Kniazev, A.

    2016-05-01

    MASTER-IAC auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 349171 ) discovered OT source at (RA, Dec) = 00h 10m 21.33s +73d 15m 16.5s on 2016-05-24.18141 UT. The OT unfiltered magnitude is 15.7m (limit 18.1m).

  1. MASTER: PSN in PGC032587 and new OTs

    NASA Astrophysics Data System (ADS)

    Shumkov, V.; Popova, E.; Lipunov, V.; Lopez, R. Rebolo; Buckley, D.; Gorbovskoy, E.; Tiurina, N.; Balanutsa, P.; Kuznetsov, A.; Kornilov, V.; Gorbunov, I.; Chazov, V.; Gress, O.; Ivanov, K.; Vladimirov, V.; Vlasenko, D.; Kuvshinov, D.; Serra-Ricart, M.; Israelyan, G.; Potter, S.; Kniazev, A.

    2016-06-01

    MASTER-IAC auto-detection system Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 349171 discovered OT source at (RA, Dec) = 10h 51m 45.85s +55d 23m 12.4s on 2016-05-31.97997 UT. The OT unfiltered magnitude is 18.3 (limit 20.1m).

  2. MASTER-IAC: very bright high amplitude OT

    NASA Astrophysics Data System (ADS)

    Gress, O.; Lipunov, V.; Lopez, R. Rebolo; Serra-Ricart, M.; Gorbovskoy, E.; Tiurina, N.; Balanutsa, P.; Kornilov, V.; Kuznetsov, A.; Kuvshinov, D.; Vlasenko, D.; Vladimirov, V.; Popova, E.; Chazov, V.; Israelyan, G.; Lodieu, N.; Budnev, N.; Ivanov, K.

    2016-07-01

    MASTER-IAC auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 20h 48m 06.68s +13d 54m 23.3s on 2016-07-24.16661 UT. The OT unfiltered magnitude is (limit 18.5m).

  3. 76 FR 7630 - Open Meeting of the OTS Minority Depository Institutions Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-10

    ... Office of Thrift Supervision Open Meeting of the OTS Minority Depository Institutions Advisory Committee... OTS Minority Depository Institutions Advisory Committee (MDIAC) will convene a meeting on Tuesday..., the Office of Thrift Supervision is announcing that the OTS Minority Depository Institutions...

  4. 75 FR 13344 - Open Meeting of the OTS Minority Depository Institutions Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-19

    ... Office of Thrift Supervision Open Meeting of the OTS Minority Depository Institutions Advisory Committee... OTS Minority Depository Institutions Advisory Committee (MDIAC) will convene a meeting on Wednesday... Supervision is announcing that the OTS Minority Depository Institutions Advisory Committee will convene...

  5. MASTER: very bright high amplitude(>7.2m) OT

    NASA Astrophysics Data System (ADS)

    Balanutsa, V.; Balanutsa, P.; Lipunov, V.; Lopez, R. Rebolo; Serra-Ricart, M.; Gorbovskoy, E.; Tiurina, N.; Kornilov, V.; Kuznetsov, A.; Kuvshinov, D.; Vlasenko, D.; Vladiirov, V.; Popova, E.; Chazov, V.; Israelyan, G.; Lodieu, N.; Budnev, N.; Gress, O.; Ivanov, K.

    2016-07-01

    MASTER-IAC auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 20h 53m 16.61s -10d 56m 33.1s on 2016-07-24.10765 UT. The OT unfiltered (W=0.2B+0.8R, calibrated by USNO-B1) magnitude is 14.8 (limit 18.7m).

  6. MASTER: OT discovered during inspection of HESE 58537957 trigger

    NASA Astrophysics Data System (ADS)

    Tyurina, N.; Lipunov, V.; Buckley, D.; Gorbovskoy, E.; Balanutsa, P.; Kuznetsov, A.; Kornilov, V.; Kuvshinov, D.; Vlasenko, D.; Gress, O.; Ivanov, K.; Shumkov, V.; Potter, S.

    2016-08-01

    MASTER-SAAO auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 349171 ) discovered OT source at (RA, Dec) = 13h 08m 45.02s -32d 32m 54.9s on 2016-08-24.73811 UT during inspection of HESE alert ( 58537957 trigger number ) http://gcn.gsfc.nasa.gov/notices_amon/58537957_128340.amon . The OT unfiltered magnitude is 19.6m (limit 20.5m).

  7. OT promotes closer interpersonal distance among highly empathic individuals

    PubMed Central

    Mankuta, David; Shamay-Tsoory, Simone G.

    2015-01-01

    The space between people, or ‘interpersonal distance’, creates and defines the dynamics of social interactions and is a salient cue signaling responsiveness and feeling comfortable. This distance is implicit yet clearly felt, especially if someone stands closer or farther away than expected. Increasing evidence suggests that Oxytocin (OT) serves as a social hormone in humans, and that one of its roles may be to alter the perceptual salience of social cues. Considering that empathic ability may shape the way individuals process social stimuli, we predicted that OT will differentially affect preferred interpersonal distance depending on individual differences in empathy. Participants took part in two interpersonal distance experiments: In the first, they had to stop a (computer visualized) protagonist when feeling most comfortable; in the second, they were asked to choose the room in which they would later discuss intimate topics with another. Both experiments revealed an interaction between the effect of OT and empathy level. Among highly empathic individuals, OT promoted the choice of closer interpersonal distances. Yet, OT had an opposite effect on individuals with low empathic traits. We conclude that the enhancement of social cues following OT administration may have opposite effects on individuals with different empathic abilities. PMID:24509491

  8. OT promotes closer interpersonal distance among highly empathic individuals.

    PubMed

    Perry, Anat; Mankuta, David; Shamay-Tsoory, Simone G

    2015-01-01

    The space between people, or 'interpersonal distance', creates and defines the dynamics of social interactions and is a salient cue signaling responsiveness and feeling comfortable. This distance is implicit yet clearly felt, especially if someone stands closer or farther away than expected. Increasing evidence suggests that Oxytocin (OT) serves as a social hormone in humans, and that one of its roles may be to alter the perceptual salience of social cues. Considering that empathic ability may shape the way individuals process social stimuli, we predicted that OT will differentially affect preferred interpersonal distance depending on individual differences in empathy. Participants took part in two interpersonal distance experiments: In the first, they had to stop a (computer visualized) protagonist when feeling most comfortable; in the second, they were asked to choose the room in which they would later discuss intimate topics with another. Both experiments revealed an interaction between the effect of OT and empathy level. Among highly empathic individuals, OT promoted the choice of closer interpersonal distances. Yet, OT had an opposite effect on individuals with low empathic traits. We conclude that the enhancement of social cues following OT administration may have opposite effects on individuals with different empathic abilities. PMID:24509491

  9. A new assessment method of outdoor tobacco smoke (OTS) exposure

    NASA Astrophysics Data System (ADS)

    Cho, Hyeri; Lee, Kiyoung

    2014-04-01

    Outdoor tobacco smoke (OTS) is concerned due to potential health effects. An assessment method of OTS exposure is needed to determine effects of OTS and validate outdoor smoking policies. The objective of this study was to develop a new method to assess OTS exposure. This study was conducted at 100 bus stops including 50 centerline bus stops and 50 roadside bus stops in Seoul, Korea. Using real-time aerosol monitor, PM2.5 was measured for 30 min at each bus stop in two seasons. ‘Peak analysis' method was developed to assess short term PM2.5 exposure by OTS. The 30-min average PM2.5 exposure at each bus stop was associated with season and bus stop location but not smoking activity. The PM2.5 peak occurrence rate by the peak analysis method was significantly associated with season, bus stop location, observed smoking occurrence, and the number of buses servicing a route. The PM2.5 peak concentration was significantly associated with season, smoking occurrence, and the number of buses servicing a route. When a smoker was standing still at the bus stop, magnitude of peak concentrations were significantly higher than when the smoker walking-through the bus stop. People were exposed to high short-term PM2.5 peak levels at bus stops, and the magnitude of peak concentrations were highest when a smoker was located close to the monitor. The magnitude of peak concentration was a good indicator helped distinguish nearby OTS exposure. Further research using ‘peak analysis' is needed to measure smoking-related exposure to PM2.5 in other outdoor locations.

  10. 12 CFR 500.10 - The OTS or The Office.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 5 2010-01-01 2010-01-01 false The OTS or The Office. 500.10 Section 500.10 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY AGENCY ORGANIZATION AND... supervision of savings associations by regulatory staff to ensure the safety and soundness of the industry....

  11. 12 CFR 574.7 - Determination by the OTS.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... SAVINGS ASSOCIATIONS § 574.7 Determination by the OTS. (a) Acquisition by a company. (1) The Office shall... section are not met. Acquisitions involving mergers with an interim association shall also be subject to... company are composed of persons who, at the time of acquisition, are executive officers and directors...

  12. 12 CFR 510.5 - Release of unpublished OTS information.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... disclosed under the Freedom of Information Act, see 5 U.S.C. 552, and 31 CFR 1.1-1.6; and (iv) Requests for... motions in the case that may bear on the asserted relevance of the information being sought from the OTS... Washington, DC, in accordance with paragraph (c) of this section. (3) Appearance by person served. Except...

  13. Teaching the Moving Child: OT Insights That Will Transform Your K-3 Classroom

    ERIC Educational Resources Information Center

    Berkey, Sybil M.

    2009-01-01

    Because sensorimotor and environmental factors have a profound effect on children's learning, every teacher should know how to weave strategies from occupational therapy (OT) into their everyday instruction. This is the guidebook K-3 teachers need to "think like an OT"--and form effective partnerships with OTs in their schools--so all students can…

  14. 75 FR 63895 - Open Meeting of the OTS Minority Depository Institutions Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-18

    ... Office of Thrift Supervision Open Meeting of the OTS Minority Depository Institutions Advisory Committee... OTS Minority Depository Institutions Advisory Committee (MDIAC) will convene a meeting on Wednesday.... SUPPLEMENTARY INFORMATION: By this notice, the Office of Thrift Supervision is announcing that the OTS...

  15. 12 CFR 563b.115 - How will OTS review my business plan?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 5 2010-01-01 2010-01-01 false How will OTS review my business plan? 563b.115... business plan? (a) OTS will review your business plan to determine that it demonstrates a safe and sound... determination, OTS will consider how you have addressed the applicable factors of § 563b.105. No single...

  16. 12 CFR 550.560 - When may the OTS revoke my fiduciary powers?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 5 2010-01-01 2010-01-01 false When may the OTS revoke my fiduciary powers? 550.560 Section 550.560 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY... § 550.560 When may the OTS revoke my fiduciary powers? The OTS may revoke your fiduciary powers if...

  17. 12 CFR 516.270 - How long is the OTS review period?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 5 2010-01-01 2010-01-01 false How long is the OTS review period? 516.270 Section 516.270 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY APPLICATION PROCESSING PROCEDURES OTS Review Standard Treatment § 516.270 How long is the OTS review period? (a)...

  18. 12 CFR 516.270 - How long is the OTS review period?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 12 Banks and Banking 6 2012-01-01 2012-01-01 false How long is the OTS review period? 516.270 Section 516.270 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY APPLICATION PROCESSING PROCEDURES OTS Review Standard Treatment § 516.270 How long is the OTS review period? (a)...

  19. 12 CFR 516.270 - How long is the OTS review period?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 12 Banks and Banking 6 2014-01-01 2012-01-01 true How long is the OTS review period? 516.270 Section 516.270 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY APPLICATION PROCESSING PROCEDURES OTS Review Standard Treatment § 516.270 How long is the OTS review period? (a)...

  20. 75 FR 38188 - Closed Meeting of the OTS Mutual Savings Association Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-01

    ... Office of Thrift Supervision Closed Meeting of the OTS Mutual Savings Association Advisory Committee.... SUMMARY: The OTS Mutual Savings Associations Advisory Committee (MSAAC) will convene a meeting on... the OTS Mutual Savings Association Advisory Committee will convene a closed meeting on Wednesday,...

  1. Interference experiment with the Sirio-OTS satellites

    NASA Astrophysics Data System (ADS)

    Lombardi, P.; Migliorini, P.; Saggese, E.

    1983-06-01

    During an orbit transfer of the Sirio satellite an interference experiment with the OTS satellite carried was performed. Both satellites may transmit in the 11.6 GHz band. A BPSK signal was transmitted to the satellites and received by stations with programmed tracking systems. Receiving stations registered the signal power intensity and the bit errors in 1 sec time samples. The satellites do not interfere if their angular distance is larger than twice the beam aperture of the receiving antenna.

  2. Optimization of OT-MACH Filter Generation for Target Recognition

    NASA Technical Reports Server (NTRS)

    Johnson, Oliver C.; Edens, Weston; Lu, Thomas T.; Chao, Tien-Hsin

    2009-01-01

    An automatic Optimum Trade-off Maximum Average Correlation Height (OT-MACH) filter generator for use in a gray-scale optical correlator (GOC) has been developed for improved target detection at JPL. While the OT-MACH filter has been shown to be an optimal filter for target detection, actually solving for the optimum is too computationally intensive for multiple targets. Instead, an adaptive step gradient descent method was tested to iteratively optimize the three OT-MACH parameters, alpha, beta, and gamma. The feedback for the gradient descent method was a composite of the performance measures, correlation peak height and peak to side lobe ratio. The automated method generated and tested multiple filters in order to approach the optimal filter quicker and more reliably than the current manual method. Initial usage and testing has shown preliminary success at finding an approximation of the optimal filter, in terms of alpha, beta, gamma values. This corresponded to a substantial improvement in detection performance where the true positive rate increased for the same average false positives per image.

  3. Fixed ground antenna radome (FGAR) type 1/3 OT&E integrations and OT&E operational

    NASA Astrophysics Data System (ADS)

    Baker, Leonard H.; Sedgwick, Harold G.

    1995-05-01

    This test report documents the Operational Test and Evaluation (OT&E) Integration and OT&E Operational testing performed on the Type I/Ill, Fixed Ground Antenna Radome (FGAR). The Type I/Ill FGAR is used with the Air Route Surveillance Radar (ARSR)) -1/2 and AN/FPS military radars. The testing was performed at the Federal Aviation Administration (FAA) Technical Center's Elwood En Route Beacon Test Facility (ERBTF) and the Northwest Mountain Region's Trinidad En Route Radar Facility (TAD), Colorado. The testing included: (1) characterization of the primary and secondary radar's electromagnetic performance; (2) human factors; (3) physical characteristics; and (4) physical performance. The electromagnetic performance testing showed no degradation of the primary or secondary radars; there were no human factor problems found; and only minor problems were identified during the physical characteristics and physical performance tests. The testing determined that the FGAR meets the Operational Suitability and Operational Effectiveness requirements of the FAA.

  4. Regulation of functional KCNQ1OT1 lncRNA by β-catenin

    PubMed Central

    Sunamura, Naohiro; Ohira, Takahito; Kataoka, Miki; Inaoka, Daigo; Tanabe, Hideyuki; Nakayama, Yuji; Oshimura, Mitsuo; Kugoh, Hiroyuki

    2016-01-01

    Long noncoding RNAs (lncRNAs) have been implicated in many biological processes through epigenetic mechanisms. We previously reported that KCNQ1OT1, an imprinted antisense lncRNA in the human KCNQ1 locus on chromosome 11p15.5, is involved in cis-limited silencing within an imprinted KCNQ1 cluster. Furthermore, aberration of KCNQ1OT1 transcription was observed with a high frequency in colorectal cancers. However, the molecular mechanism of the transcriptional regulation and the functional role of KCNQ1OT1 in colorectal cancer remain unclear. Here, we show that the KCNQ1OT1 transcriptional level was significantly increased in human colorectal cancer cells in which β-catenin was excessively accumulated in the nucleus. Additionally, overexpression of β-catenin resulted in an increase in KCNQ1OT1 lncRNA-coated territory. On the other hand, knockdown of β-catenin resulted in significant decrease of KCNQ1OT1 lncRNA-coated territory and an increase in the mRNA expression of the SLC22A18 and PHLDA2 genes that are regulated by KCNQ1OT1. We showed that β-catenin can promote KCNQ1OT1 transcription through direct binding to the KCNQ1OT1 promoter. Our evidence indicates that β-catenin signaling may contribute to development of colorectal cancer by functioning as a novel lncRNA regulatory factor via direct targeting of KCNQ1OT1. PMID:26868975

  5. Complex architecture and regulated expression of the Sox2ot locus during vertebrate development.

    PubMed

    Amaral, Paulo P; Neyt, Christine; Wilkins, Simon J; Askarian-Amiri, Marjan E; Sunkin, Susan M; Perkins, Andrew C; Mattick, John S

    2009-11-01

    The Sox2 gene is a key regulator of pluripotency embedded within an intron of a long noncoding RNA (ncRNA), termed Sox2 overlapping transcript (Sox2ot), which is transcribed in the same orientation. However, this ncRNA remains uncharacterized. Here we show that Sox2ot has multiple transcription start sites associated with genomic features that indicate regulated expression, including highly conserved elements (HCEs) and chromatin marks characteristic of gene promoters. To identify biological processes in which Sox2ot may be involved, we analyzed its expression in several developmental systems, compared to expression of Sox2. We show that Sox2ot is a stable transcript expressed in mouse embryonic stem cells, which, like Sox2, is down-regulated upon induction of embryoid body (EB) differentiation. However, in contrast to Sox2, Sox2ot is up-regulated during EB mesoderm-lineage differentiation. In adult mouse, Sox2ot isoforms were detected in tissues where Sox2 is expressed, as well as in different tissues, supporting independent regulation of expression of the ncRNA. Sox2dot, an isoform of Sox2ot transcribed from a distal HCE located >500 kb upstream of Sox2, was detected exclusively in the mouse brain, with enrichment in regions of adult neurogenesis. In addition, Sox2ot isoforms are transcribed from HCEs upstream of Sox2 in other vertebrates, including in several regions of the human brain. We also show that Sox2ot is dynamically regulated during chicken and zebrafish embryogenesis, consistently associated with central nervous system structures. These observations provide insight into the structure and regulation of the Sox2ot gene, and suggest conserved roles for Sox2ot orthologs during vertebrate development.

  6. 12 CFR 502.20 - How does OTS determine my condition component?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 5 2010-01-01 2010-01-01 false How does OTS determine my condition component? 502.20 Section 502.20 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY ASSESSMENTS AND FEES Assessments Savings Associations-Calculation of Assessments § 502.20 How does OTS determine my condition component? (a) If you are...

  7. MASTER-Kislovodsk: bright OT (PSN or CV, ampl>5.2m)

    NASA Astrophysics Data System (ADS)

    Kuznetsov, N.; Shumkov, V.; Lipunov, V.; Tlatov, A.; Rebolo, R.; Serra-Ricart, M.; Senik, V.; Gorbovskoy, E.; Tiurina, N.; Balanutsa, P.; Kuznetsov, A.; Kornilov, V.; Vladimirov, V.; Vlasenko, D.; Dormidontov, D.; Parkhomenko, A.; Gress, O.; Ivanov, K.; Budnev, N.

    2016-10-01

    MASTER-Kislovodsk auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 10h 06m 29.13s +22d 26m 43.8s with unfiltered m_OT=16.8m on 2016-10-09 00:56:53.746UT.

  8. 12 CFR 502.60 - When will OTS adjust, add, waive, or eliminate a fee?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 5 2010-01-01 2010-01-01 false When will OTS adjust, add, waive, or eliminate... TREASURY ASSESSMENTS AND FEES Fees § 502.60 When will OTS adjust, add, waive, or eliminate a fee? Under unusual circumstances, the Director may deem it necessary or appropriate to adjust, add, waive,...

  9. 12 CFR 502.60 - When will OTS adjust, add, waive, or eliminate a fee?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 12 Banks and Banking 5 2011-01-01 2011-01-01 false When will OTS adjust, add, waive, or eliminate... TREASURY ASSESSMENTS AND FEES Fees § 502.60 When will OTS adjust, add, waive, or eliminate a fee? Under unusual circumstances, the Director may deem it necessary or appropriate to adjust, add, waive,...

  10. 12 CFR 555.300 - Must I inform OTS before I use electronic means or facilities?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 5 2010-01-01 2010-01-01 false Must I inform OTS before I use electronic means... THE TREASURY ELECTRONIC OPERATIONS Requirements Applicable to All Savings Associations § 555.300 Must I inform OTS before I use electronic means or facilities? (a) General. A savings association...

  11. The 1000-th MASTER detection and SALT limit: Fast Doublet OT

    NASA Astrophysics Data System (ADS)

    Balanutsa, P.; Lipunov, V.; Gorbovskoy, E.; Buckley, D.; Kniazev, A.; Tiurina, N.; Kornilov, V.; Samus, N.; Kuznetsov, A.; Vlasenko, D.; Gorbunov, I.; Popova, E.; Vladimirov, V.; Shumkov, V.; Potter, S.; Kotze, M.; Gress, O.; Budnev, N.; Yazev, S.; Ivanov, K.; Tlatov, A.; Senik, V.; Dormidontov, D.; Parhomenko, A. V.; Yurkov, V.; Sergienko, Yu.; Gabovich, A.; Sinyakov, E.; Rebolo, R.; Serra-Ricart, M.; Lodieu, N.; Israelian, G.; Suarez-Andres, L.; Levato, Hugo; Astronomicas, Carlos Saffe Instituto de Ciencias; Espacio, de la Tierra y. del; Podesta, Ricardo; Mallamaci, Claudio; Lopez, Carlos; Podesta, Federico

    2015-12-01

    MASTER-SAAO (Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 349171) discovered the 1000th OT source at (RA, Dec) = 05h 10m 14.58s -29d 09m 00.6s on 2015-12-16.97115 UT. The OT unfiltered magnitude is 18.8m (the limit is 20.4m).

  12. 12 CFR 516.10 - How does OTS compute time periods under this part?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 12 Banks and Banking 5 2011-01-01 2011-01-01 false How does OTS compute time periods under this part? 516.10 Section 516.10 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY APPLICATION PROCESSING PROCEDURES § 516.10 How does OTS compute time periods under this part? In...

  13. 12 CFR 516.10 - How does OTS compute time periods under this part?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 5 2010-01-01 2010-01-01 false How does OTS compute time periods under this part? 516.10 Section 516.10 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY APPLICATION PROCESSING PROCEDURES § 516.10 How does OTS compute time periods under this part? In...

  14. 75 FR 24775 - Open Meeting of the OTS Mutual Savings Association Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-05

    ... Office of Thrift Supervision Open Meeting of the OTS Mutual Savings Association Advisory Committee AGENCY... Savings Associations Advisory Committee (MSAAC) will convene a telephonic meeting on Friday, May 21, 2010..., the Office of Thrift Supervision is announcing that the OTS Mutual Savings Association...

  15. 75 FR 76524 - Closed Meeting of the OTS Mutual Savings Association Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-08

    ... Office of Thrift Supervision Closed Meeting of the OTS Mutual Savings Association Advisory Committee.... SUMMARY: The OTS Mutual Savings Associations Advisory Committee (MSAAC) will convene a meeting on Monday... Savings Association Advisory Committee will convene a closed meeting on Monday, December 20,...

  16. 75 FR 61572 - Open Meeting of the OTS Mutual Savings Association Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-05

    ... Office of Thrift Supervision Open Meeting of the OTS Mutual Savings Association Advisory Committee AGENCY... Savings Associations Advisory Committee (MSAAC) will convene a meeting on Wednesday, October 20, 2010... the OTS Mutual Savings Association Advisory Committee will convene a meeting on Wednesday, October...

  17. 12 CFR 502.26 - How does OTS calculate the semi-annual assessment for savings and loan holding companies?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ...-site supervision of a noncomplex, low risk savings and loan holding company structure. OTS will... company is the registered holding company at the highest level of ownership in a holding company structure, unless OTS designates another savings and loan holding company in the holding company structure. OTS...

  18. 12 CFR 550.70 - Must I obtain OTS approval or file a notice before I exercise fiduciary powers?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... before I exercise fiduciary powers? 550.70 Section 550.70 Banks and Banking OFFICE OF THRIFT SUPERVISION... I obtain OTS approval or file a notice before I exercise fiduciary powers? You should refer to the following chart to determine if you must obtain OTS approval or file a notice with OTS before you...

  19. 12 CFR 550.70 - Must I obtain OTS approval or file a notice before I exercise fiduciary powers?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... before I exercise fiduciary powers? 550.70 Section 550.70 Banks and Banking OFFICE OF THRIFT SUPERVISION... I obtain OTS approval or file a notice before I exercise fiduciary powers? You should refer to the following chart to determine if you must obtain OTS approval or file a notice with OTS before you...

  20. 12 CFR 550.70 - Must I obtain OTS approval or file a notice before I exercise fiduciary powers?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... before I exercise fiduciary powers? 550.70 Section 550.70 Banks and Banking OFFICE OF THRIFT SUPERVISION... I obtain OTS approval or file a notice before I exercise fiduciary powers? You should refer to the following chart to determine if you must obtain OTS approval or file a notice with OTS before you...

  1. 12 CFR 550.70 - Must I obtain OTS approval or file a notice before I exercise fiduciary powers?

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... before I exercise fiduciary powers? 550.70 Section 550.70 Banks and Banking OFFICE OF THRIFT SUPERVISION... I obtain OTS approval or file a notice before I exercise fiduciary powers? You should refer to the following chart to determine if you must obtain OTS approval or file a notice with OTS before you...

  2. 12 CFR 550.70 - Must I obtain OTS approval or file a notice before I exercise fiduciary powers?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... before I exercise fiduciary powers? 550.70 Section 550.70 Banks and Banking OFFICE OF THRIFT SUPERVISION... I obtain OTS approval or file a notice before I exercise fiduciary powers? You should refer to the following chart to determine if you must obtain OTS approval or file a notice with OTS before you...

  3. 12 CFR 550.100 - What factors may the OTS consider in its review of my application?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 12 Banks and Banking 5 2011-01-01 2011-01-01 false What factors may the OTS consider in its review... factors may the OTS consider in its review of my application? The OTS may consider the following factors.... (g) The needs of the community to be served. (h) Any other facts or circumstances that the...

  4. 12 CFR 550.100 - What factors may the OTS consider in its review of my application?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 12 Banks and Banking 6 2014-01-01 2012-01-01 true What factors may the OTS consider in its review... factors may the OTS consider in its review of my application? The OTS may consider the following factors.... (g) The needs of the community to be served. (h) Any other facts or circumstances that the...

  5. 12 CFR 550.100 - What factors may the OTS consider in its review of my application?

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 12 Banks and Banking 6 2013-01-01 2012-01-01 true What factors may the OTS consider in its review... factors may the OTS consider in its review of my application? The OTS may consider the following factors.... (g) The needs of the community to be served. (h) Any other facts or circumstances that the...

  6. A Study of DNA Adsorption Kinetics on OTS Surfaces

    NASA Astrophysics Data System (ADS)

    Barone, Joseph; Fang, Xiaohua; Li, Bingquan; Seo, Young-Soo; Samuilov, Vladimir; Rafailovich, Miriam; Sokolov, Jonathan

    2003-03-01

    The evaporation kinetics of droplets containing DNA were studied as a function of DNA molecular weight, DNA concentration, and buffer concentration.The contact angle and overall droplet morphology were observed using a KSV contact angle goniometer as a function of time. Simultaneously, the DNA distribution and adsorption kinetics were measured with confocal microscopy. The DNA droplets were deposited on hydrophobic OTS-covered silicon surfaces and stained with ethidium bromide solution. Up to three stages were found during DNA droplet drying process, depending on the DNA concentration. The results also show that a ring is formed at the air/solid /liquid interface in a manner similar to that reported for a colloidal suspension by Robert D. Deegan et.a. [Physical Review E, Vol 62, No.1, July 2000, p756-765] The absorbed amount of DNA was obtained by measuring the intensity in the ring. The dynamics and DNA morphology are affected by both the molecular weight and the DNA concentration. Supported by NSF-MRSEC program (DMR-9632525)

  7. OT2_ccodella_2: Where is chlorine in shocked regions?

    NASA Astrophysics Data System (ADS)

    Codella, C.

    2011-09-01

    As part of the GT Herschel Program CHESS we detected for the first time hydrogen chlorine in a protostellar shock, L1157-B1 (Codella et al. 2011). One of the most surprising results of this work was the lack of enhancement in the abundance of HCl with respect to dense interstellar clouds, implying that HCl is not enhanced by the passage of a shock. This means that either chlorine is not sputtered during the passage of the shock (unlikely as Si is sputtered) or that HCl is not the main reservoir of clorine in shocked regions (unlike in dense interstellar clouds). In this proposal we propose to observe HCl in a sample of shocked regions in order to determine whether this result is unique to L1157-B1. We stress that given the weakness of the HCl emission in shocks and the strong atmospheric water absorption at the requested frequency (626 GHz), the present experiment cannot be reasonably performed from ground, making of Herschel OT2 the last chance to reach the present goals.

  8. 12 CFR 516.250 - Will OTS require me to publish a new public notice?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... application, you submitted new or additional information, or a major issue of law or a change in circumstances... matters is appropriate because of the significance of the new information or circumstances. (b) OTS...

  9. First report on the occurrence of Theileria sp. OT3 in China.

    PubMed

    Tian, Zhancheng; Liu, Guangyuan; Yin, Hong; Xie, Junren; Wang, Suyan; Yuan, Xiaosong; Wang, Fangfang; Luo, Jin

    2014-04-01

    Theileria sp. OT3 was firstly detected and identified from clinically healthy sheep in Xinjiang Uygur Autonomous Region of China (XUAR) through comparing the complete 18S rDNA gene sequences available in GenBank database and the phylogenetic status based on the internal transcribed spacers (ITS1, ITS2) as well as the intervening 5.8S coding region of the rRNA gene by the methods of a partitioned multi-locus analysis in BEAST and Maximum likelihood analysis in PhyML. Moreover, the findings were confirmed by the species-specific PCR for Theileria sp. OT3 and the prevalence of Theileria sp. OT3 was 14.9% in the north of XUAR. This study is the first report on the occurrence of Theileria sp. OT3 in China.

  10. MASTER discovery: blue bright OT during FERMI 496473540 (GRB160925A) inspection

    NASA Astrophysics Data System (ADS)

    Gress, O.; Lipunov, V.; Buckley, D.; Tiurina, N.; Gorbovskoy, E.; Balanutsa, P.; Kuznetsov, A.; Kornilov, V.; Vladimirov, V.; Shumkov, V.; Pogrosheva, T.; Rebolo Lopez, R.; Serra Ricart, M.; Podesta, R.; Levato, H.; Potter, S.; Lodieu, N.; Saffe, C.; Lopez, C.; Podesta, F.

    2016-09-01

    MASTER-SAAO auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 22h 28m 17.90s -14d 56m 57.4s on 2016-09-25.75053 UT during automatically inspection of Fermi trigger 496473540 ( http://gcn.gsfc.nasa.gov/other/496473540.fermi ). The OT unfiltered magnitude is (limit 19.7m).

  11. Effects of Hypergravity Exposure on Plasma Oxytocin (OT) Concentrations in Pregnant and Lactating Rat Dams

    NASA Technical Reports Server (NTRS)

    Baer, Lisa A.; Wade, Charles E.; Plaut, Karen; Ronca, April E.; Dalton, Bonnie (Technical Monitor)

    2002-01-01

    From pregnancy to weaning there is a progressive elevation of plasma oxytocin (OT) levels associated with nursing activity, irrespective of litter size. In the present study, we analyzed the effects of continuous 1.5G, 1.75G and 2.0G hypergravity exposure on OT plasma concentration in prepartum (Gestation Day 20) (G20) and lactating (Postnatal day) (P10) rat dams. For this study, litter size was controlled with a yoking procedure established in our lab where individual control litters were yoked-matched to individual hypergravity litters. We reviewed all data at hypergravity irrespective of gravitational level and compared the values with the controls in both G20 (HG, n=15;SC, n=9) and P10 (HG, n=21;SC, n=16). Results showed that over time, we did observe the expected OT increase in both groups. In G20 dams, measurement of OT concentrations showed no significance. However, at P10, measurements of OT concentrations suggest a reduction of about 20% compared to established controls in our laboratory, 0.9+/-0.09 ng/ml for the controls and 0.7+/-0.06 ng/ml for centrifuged animals (p<0.02). These data suggest that exposure to centrifugation may reduce OT levels during lactation. When these plasma samples were obtained, the dams were removed from the litters, and values were not adjusted for the size of the litters. The reduction in OT with centrifugation may reflect a decrease in nursing activity or a decreased responsiveness of the mammary hypothalamic axis. In addition, we have analyzed data on plasma prolactin concentrations and mammary gland development, which may give additional insight to the results of our OT measurements.

  12. OT2_smalhotr_3: Herschel Extreme Lensing Line Observations (HELLO)

    NASA Astrophysics Data System (ADS)

    Malhotra, S.

    2011-09-01

    We request 59.8 hours of Herschel time to observe 20 normal star-forming galaxies in the [CII] 158 micron and [OI] 63 micron lines. These galaxies lie at high redshift (1OT1, 49 high redshift IR luminous galaxies were approved for spectroscopy, but only two so-called normal galaxies were included. This is an imbalance that should be corrected, to balance Herschel's legacy.

  13. OmniTread OT-4 serpentine robot: new features and experiments

    NASA Astrophysics Data System (ADS)

    Borenstein, Johann; Hansen, Malik

    2007-04-01

    Serpentine robots are slender, multi-segmented vehicles designed to provide greater mobility than conventional wheeled or tracked robots. Serpentine robots are thus ideally suited for urban search and rescue, military intelligence gathering, and for surveillance and inspection tasks in hazardous and hard-to-reach environments. One such serpentine robot, developed at the University of Michigan, is the "OmniTread OT-4." The OT-4 comprises seven segments, which are linked to each other by 2-degree-of-freedom joints. The OT-4 can climb over obstacles that are much higher than the robot itself, propel itself inside pipes of different diameters, and traverse even the most difficult terrain, such as rocks or the rubble of a collapsed structure. The foremost and unique design characteristic of the OT-4 is the use of pneumatic bellows to actuate the joints. These bellows allow simultaneous control of position and stiffness for each joint. Controllable stiffness is of crucial importance in serpentine robots, which require stiff joints to cross gaps and compliant joints to conform to rough terrain for effective propulsion. Another unique feature of the OmniTread design is the maximal coverage of all four sides with driven tracks. This design makes the robot indifferent to roll-overs, which are happen frequently when the slender bodies of serpentine robots travel over rugged terrain. This paper describes the OmniTread concept as well as its latest technical features, and an extensive Experiment Results Section documents the abilities of the OT-4.

  14. Relating material surface heterogeneity to protein adsorption: the effect of annealing of micro-contact-printed OTS patterns

    PubMed Central

    HODGKINSON, GERALD; HLADY, VLADIMIR

    2009-01-01

    We have investigated the influence of micrometer- and sub-micrometer-scale surface heterogeneities in patterned octadecyltrichlorosilane (OTS) films on human serum albumin (HSA) adsorption and its spatial distribution. 5-μm-wide OTS patterns were created on glass substrates by micro-contact printing and in some instances subsequent annealing was used to alter OTS molecule distribution within the patterns. Scanning force microscopy (SFM), advancing water contact angles and water vapor condensation figures were used to characterize the OTS films and to assess the nature of the heterogeneities within the various surface areas. High-resolution fluorescence microscopy was used to record images of fluorescently labeled albumin on OTS patterned films and fluorescence intensity was quantified and converted into the adsorbed amount. Adsorbed albumin was also characterized through SFM measurements. Combined SFM topography and lateral force microscopy (LFM) imaging revealed that micro-contact printing of OTS onto glass both replicated the stamp pattern and created small islands within the non-stamped regions between the patterns. The OTS coverage within stamped regions was not fully continuous but improved with subsequent annealing. Annealing also resulted in OTS island growth within the non-stamped regions and decreased average wettability on both the stamped and non-stamped areas. The extent of albumin adsorption was not proportional to OTS coverage, but correlated with the sub-μm distribution of OTS chains. We inferred that the surface distribution of ligands such as OTS on a sub-μm length scale determines the nature of albumin adsorption and its kinetics. PMID:19693285

  15. MASTER OT J000008.72-334156.5 is probably a M-star flare

    NASA Astrophysics Data System (ADS)

    Cao, Y.; Blagorodnova, N.; Cenko, S. B.; MIll, A.

    2015-10-01

    Following ATel #8166, we obtained a 300sec R-band image at the location of MASTER OT J000008.72-334156.5 with DEIMOS mounted on the Keck 2 telescope on 2015-10-18 UT and found a point-like source with R=21.7 mag. We also noticed a source at the same location in WISE with W1=16.6 mag. Therefore, we conclude that MASTER OT J000008.72-334156.5 is probably a galactic M-star flare.

  16. A global outer-rise/outer-trench-slope (OR/OTS) earthquake study

    NASA Astrophysics Data System (ADS)

    Wartman, J. M.; Kita, S.; Kirby, S. H.; Choy, G. L.

    2009-12-01

    Using improved seismic, bathymetric, satellite gravity and other geophysical data, we investigated the seismicity patterns and focal mechanisms of earthquakes in oceanic lithosphere off the trenches of the world that are large enough to be well recorded at teleseismic distances. A number of prominent trends are apparent, some of which have been previously recognized based on more limited data [1], and some of which are largely new [2-5]: (1) The largest events and the highest seismicity rates tend to occur where Mesozoic incoming plates are subducting at high rates (e.g., those in the western Pacific and the Banda segment of Indonesia). The largest events are predominantly shallow normal faulting (SNF) earthquakes. Less common are reverse-faulting (RF) events that tend to be deeper and to be present along with SNF events where nearby seamounts, seamount chains and other volcanic features are subducting [Seno and Yamanaka, 1996]. Blooms of SNF OR/OTS events usually occur just after and seaward of great interplate thrust (IPT) earthquakes but are far less common after smaller IPT events. (2) Plates subducting at slow rates (<20 mm/a) often show sparse OR/OTS seismicity. It is unclear if such low activity is a long-term feature of these systems or is a consequence of the long return times of great IPT earthquakes (e.g., the sparse OR/OTS seismicity before the 26 December 2004 M9.2 Sumatra earthquake and many subsequent OR/OTS events). (3) OR/OTS shocks are generally sparse or absent where incoming plates are very young (<20 Ma) (e.g., Cascadia, southern Mexico, Nankai, and South Shetlands). (4) Subducting plates of intermediate age (20 to about 65 Ma) display a diversity of focal mechanisms and seismicity patterns. In the Philippines, NE Indonesia, and Melanesia, bands of reverse faulting events occur at or near the trench and SNF earthquakes are restricted to OR/OTS sites further from the trench. (5) Clustering of OR/OTS events of all types commonly occurs where

  17. The peptide Z-Aib-Aib-Aib-L-Ala-OtBu.

    PubMed

    Gessmann, Renate; Brückner, Hans; Petratos, Kyriacos

    2014-04-01

    The title peptide, N-benzyloxycarbonyl-α-aminoisobutyryl-α-aminoisobutyryl-α-aminoisobutyryl-L-alanine tert-butyl ester or Z-Aib-Aib-Aib-L-Ala-OtBu (Aib is α-aminoisobutyric acid, Z is benzyloxycarbonyl and OtBu indicates the tert-butyl ester), C27H42N4O7, is a left-handed helix with a right-handed conformation in the fourth residue, which is the only chiral residue. There are two 4→1 intramolecular hydrogen bonds in the structure. In the lattice, molecules are hydrogen bonded to form columns along the c axis.

  18. The achiral tetrapeptide Z-Aib-Aib-Aib-Gly-OtBu.

    PubMed

    Gessmann, Renate; Brückner, Hans; Petratos, Kyriacos

    2014-11-01

    The title achiral peptide N-benzyloxycarbonyl-α-aminoisobutyryl-α-aminoisobutyryl-α-aminoisobutyrylglycine tert-butyl ester or Z-Aib-Aib-Aib-Gly-OtBu (Aib is α-aminoisobutyric acid, Z is benzyloxycarbonyl, Gly is glycine and OtBu indicates the tert-butyl ester), C26H40N4O7, is partly hydrated (0.075H2O) and has two different conformations which together constitute the asymmetric unit. Both molecules form incipient 310-helices. They differ in the relative orientation of the N-terminal protection group and at the C-terminus. There are two 4→1 intramolecular hydrogen bonds.

  19. MASTER-OAFA discovery: bright high amplitude(>7.5m) OT and PSN in bright spiral pgc131693in

    NASA Astrophysics Data System (ADS)

    Pogrosheva, T.; Gress, O.; Lipunov, V.; Podesta, R.; Levato, H.; Gorbovskoy, E.; Tyurina, N.; Balanutsa, P.; Kuznetsov, A.; Kornilov, V.; Kuvshinov, D.; Vladimirov, V.; Gress, O.; Ivanov, K.; Lopez, C.; Lopez, C.; Podesta, F

    2016-09-01

    MASTER-OAFA auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 22h 33m 13.74s -40d 38m 36.9s on 2016-09-19.12230 UT. The OT unfiltered magnitude is (mlim=18.4m).

  20. Long non-coding RNA SOX2OT: expression signature, splicing patterns, and emerging roles in pluripotency and tumorigenesis

    PubMed Central

    Shahryari, Alireza; Jazi, Marie Saghaeian; Samaei, Nader M.; Mowla, Seyed J.

    2015-01-01

    SOX2 overlapping transcript (SOX2OT) is a long non-coding RNA which harbors one of the major regulators of pluripotency, SOX2 gene, in its intronic region. SOX2OT gene is mapped to human chromosome 3q26.3 (Chr3q26.3) locus and is extended in a high conserved region of over 700 kb. Little is known about the exact role of SOX2OT; however, recent studies have demonstrated a positive role for it in transcription regulation of SOX2 gene. Similar to SOX2, SOX2OT is highly expressed in embryonic stem cells and down-regulated upon the induction of differentiation. SOX2OT is dynamically regulated during the embryogenesis of vertebrates, and delimited to the brain in adult mice and human. Recently, the disregulation of SOX2OT expression and its concomitant expression with SOX2 have become highlighted in some somatic cancers including esophageal squamous cell carcinoma, lung squamous cell carcinoma, and breast cancer. Interestingly, SOX2OT is differentially spliced into multiple mRNA-like transcripts in stem and cancer cells. In this review, we are describing the structural and functional features of SOX2OT, with an emphasis on its expression signature, its splicing patterns and its critical function in the regulation of SOX2 expression during development and tumorigenesis. PMID:26136768

  1. 12 CFR 516.220 - If OTS requests additional information to complete my application, how will it process my...

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... complete my application, how will it process my application? 516.220 Section 516.220 Banks and Banking... Standard Treatment § 516.220 If OTS requests additional information to complete my application, how will it... your response. OTS will notify you that it has extended the period before the end of the initial...

  2. 12 CFR 516.220 - If OTS requests additional information to complete my application, how will it process my...

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... complete my application, how will it process my application? 516.220 Section 516.220 Banks and Banking... Standard Treatment § 516.220 If OTS requests additional information to complete my application, how will it... your response. OTS will notify you that it has extended the period before the end of the initial...

  3. Optimal Diphthongs: An OT Analysis of the Acquisition of Spanish Diphthongs

    ERIC Educational Resources Information Center

    Krause, Alice

    2013-01-01

    This dissertation investigates the acquisition of Spanish diphthongs by adult native speakers of English. The following research questions will be addressed: 1) How do adult native speakers of English pronounce sequences of two vowels in their L2 Spanish at different levels of acquisition? 2) Can OT learnability models, specifically the GLA,…

  4. OGLE-IV Pre-discovery Observations of MASTER OT J010603.18-744715.8

    NASA Astrophysics Data System (ADS)

    Mroz, P.; Udalski, A.

    2016-10-01

    Shumkov et al. (ATel #9621) reported the discovery of a bright optical transient MASTER OT J010603.18-744715.8 toward the Small Magellanic Cloud. The object is located in the fields SMC727 and SMC734 that are regularly monitored by the OGLE-IV Survey.

  5. Spectroscopy and photometry of MASTER OT J004126.22+414350.0 in the Andromeda direction

    NASA Astrophysics Data System (ADS)

    Williams, S. C.; Hornoch, K.; Henze, M.; Darnley, M. J.

    2016-09-01

    MASTER OT J004126.22+414350.0 was discovered on 2016 Sep 02.12339 UT (ATel #9470). The transient is coincident with M31N 2013-11b (ATel #9470), which was originally identified as an M31 nova candidate (ATel #5569), but later found to be more likely a red LPV (ATel #5640, #5744).

  6. MASTER: young PSN(Ia) in bright PGC054958 and OTs

    NASA Astrophysics Data System (ADS)

    Shumkov, V.; Shurpakov, S.; Lipunov, V.; Lopez, R. Rebolo; Buckley, D.; Ricart, Serra; Israelyan, G.; Potter, S.; Kniazev, A.; Gorbovskoy, E.; Tiurina, N.; Balanutsa, P.; Kuznetsov, A.; Kornilov, V.; Vlasenko, D.; Gorbunov, I.; Kuvshinov, D.; Vladimirov, V.; Popova, E.

    2016-04-01

    MASTER-IAC auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 349171 ) discovered OT source at (RA, Dec) = 15h 23m 33.04s +09d 21m 25.6s on 2016-04-04.09646 UT. PSN unfiltered magnitude is 17.7m (limit 19.5m).

  7. 78 FR 54403 - Removal of Transferred OTS Regulations Regarding Recordkeeping and Confirmation Requirements for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-04

    ...\\ \\2\\ 76 FR 39247 (July 6, 2011). Although section 312(b)(2)(B)(i)(II) of the Dodd-Frank Act, codified... them, as appropriate. \\3\\ 76 FR 47652 (Aug. 5, 2011). One of the OTS's rules transferred to the FDIC... confirmation requirements in appropriate circumstance.\\6\\ \\4\\ 44 FR 43260, 43261 (July 24, 1979). \\5\\ See 44...

  8. 12 CFR 502.27 - How does OTS determine the risk/complexity component for a savings and loan holding company?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 5 2010-01-01 2010-01-01 false How does OTS determine the risk/complexity...-Calculation of Assessments § 502.27 How does OTS determine the risk/complexity component for a savings and loan holding company? (a) OTS computes the risk/complexity component for responsible savings and...

  9. Distribution of polyphenols and a surfactant component in skin during Aerosol OT microemulsion-enhanced intradermal delivery.

    PubMed

    Yutani, Reiko; Morita, Shin-ya; Teraoka, Reiko; Kitagawa, Shuji

    2012-01-01

    As for most other polyphenols, intradermal delivery of curcumin and resveratrol is limited; however, it was significantly improved by a microemulsion using Aerosol OT (Aerosol OT microemulsion) and Tween 80 (Tween 80 microemulsion) as surfactants. Aerosol OT microemulsion was more effective and the incorporation ratio of these polyphenols into skin by Aerosol OT microemulsion was five-fold or ten-fold that by Tween 80 microemulsion. To clarify the mechanism of the enhancement we examined the distribution of these polyphenols and the surfactant component, Aerosol OT, using excised guinea pig skin and Yucatan micropig (YMP) skin. During permeation, polyphenols distributed deep in the skin. In particular, a small molecule, resveratrol, was mainly present in the dermis in YMP skin. Aerosol OT also distributed deep in the skin. These findings suggest the possible involvement of the interaction of surfactant molecules with skin components in the enhanced delivery process of polyphenols. The distribution ratio between the dermis and epidermis of the polyphenols, including quercetin, in the presence of Aerosol OT microemulsion decreased with the increase of molecular weight in YMP skin, suggesting the possibility that distribution to the dermis is regulated by the molecular size.

  10. Spectral Classification of MASTER OT J015539.85+485955.6 as a dwarf nova

    NASA Astrophysics Data System (ADS)

    Piascik, A. S.; Steele, I. A.

    2015-11-01

    MASTER OT J015539.85+485955.6 was identified as either a possible supernova candidate in PGC212862 or a galactic dwarf nova in outburst in Atel #8262. The transient was observed using the SPRAT spectrograph of the Liverpool Telescope (La Palma) with a spectral range of 4000 - 8000 Angstroms and a resolution R=350 on 2015-11-09 at 22:40UT.

  11. MASTER-SAAO: bright PSN in PGC600519 and 2 OTs

    NASA Astrophysics Data System (ADS)

    Pogrosheva, T.; Balanutsa, P.; Lipunov, V.; Buckley, D.; Gorbovskoy, E.; Tiurina, N.; Gress, O.; Shumkov, V.; Kuznetsov, A.; Kornilov, V.; Chazov, V.; Vladimirov, V.; Vlasenko, D.; Potter, S.

    2016-10-01

    MASTER-SAAO auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 10h 54m 40.86s , -39d 13m 19s.0 on 2016-10-08 02:56:46.019UT with . This PSN is in 2.9"E,7"N from the center of PGC600519 and is seen on 8 images.

  12. 78 FR 76768 - Removal of Transferred OTS Regulations Regarding Disclosure and Reporting of CRA-Related...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-19

    ... on July 6, 2011.\\2\\ \\2\\ 76 FR 39247 (July 6, 2011). Although section 312(b)(2)(B)(i)(II) of the Dodd... rescinding them, as appropriate. \\3\\ 76 FR 47652 (Aug. 5, 2011). One of the OTS rules transferred to the FDIC...)(1). \\8\\ 66 FR 2052 (Jan. 10, 2001). \\9\\ Id. Section 48 of the FDI Act, created by section 711 of...

  13. Asiago spectroscopic classification of MASTER OT J211223.35+144645.1

    NASA Astrophysics Data System (ADS)

    Ascenzi, S.; Yang, S.; Tomasella, L.; Benetti, S.; Cappellaro, E.; Pastorello, A.; Elias-Rosa, N.; Ochner, P.; Tartaglia, L.; Terreran, G.; Turatto, M.

    2016-06-01

    The Asiago Transient Classification Program (Tomasella et al. 2014, AN, 335, 841) reports the spectroscopic classification of MASTER OT J211223.35+144645.1 (Atel #9174), discovered by MASTER-IAC auto-detection system (Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 349171) The observation was performed with the Asiago 1.82 m Copernico Telescope (+AFOSC; range 340-820 nm; resolution 1.4 nm).

  14. Identification of new SOX2OT transcript variants highly expressed in human cancer cell lines and down regulated in stem cell differentiation.

    PubMed

    Saghaeian Jazi, Marie; Samaei, Nader Mansour; Ghanei, Mostafa; Shadmehr, Mohammad Behgam; Mowla, Seyed Javad

    2016-02-01

    Long non-coding RNAs are manifested as a new paradigm of molecular effectors in a wide range of human diseases. Human SOX2 overlapping transcript (SOX2OT) gene can generate six lncRNA transcript variants which are functionally assumed to be correlated with cellular differentiation and carcinogenesis. However, the circumstances determining expressional and functional differences between SOX2OT transcript variants remain to be explored. Here, we studied the expression of all SOX2OT transcript variants specifically in five human cancer cell lines by real-time RT-PCR. Changes of the new SOX2OT transcript variants expression were measured during the NT2 teratocarcinoma cell line neuronal-like differentiation and were compared to pluripotency regulators, SOX2 and OCT4A gene expressions. Surprisingly, we identified two new SOX2OT transcripts, named SOX2OT-7, SOX2OT-8 which lack exon 8. We discovered that beside active proximal and distal SOX2OT promoters, different cancer cell lines express high levels of some SOX2OT transcript variants differentially by alternative splicing. Significantly, both SOX2OT-7 and SOX2OT-8 are highly expressed in human cancer cell lines coinciding with SOX2, one of the pluripotency regulators. Our results revealed that SOX2OT-7 is almost the most abundant form of SOX2OT transcript variants in the examined cancer cell lines particularly in NT2 teratocarcinoma cell line where its expression falls upon neuronal-like differentiation similar to SOX2 and OCT4A. We suggest that at least some of SOX2OT transcripts are significantly associated with cancer and stem cell related pathways.

  15. MASTER OT J095503.19-355149.4 - a probable Dwarf Nova

    NASA Astrophysics Data System (ADS)

    Buckley, D. A. H.; Kniazev, A.; Potter, S. B.; Lipunov, V.; Tiurina, N.; Gorbovskoy, E.

    2016-02-01

    Followup optical imaging and spectroscopic observations with SALT of the optical transient MASTER OT J095503.19-355149.4, tentatively identified (ATel #8741) as the optical counterpart of the Fermi GBM trigger 478121068 (GCN #19092), were conducted on 2016-02-26.78 UT. From un-calibrated g', r' and i' exposures (30 sec each) with the SALTICAM CCD camera, the transient appeared to be approximately 1 mag brighter (V ~ 17) than the MASTER-SAAO observations of the previous night (reported in ATel #8741) and was also seen to be a relatively red object compared to other stars in the field.

  16. Spectroscopic classification of PSN J13144705+5405055 (= MASTER OT J131447.05+540505.5)

    NASA Astrophysics Data System (ADS)

    Vanni, S.; Pastorello, A.; Benetti, S.; Cappellaro, E.; Ochner, P.; Tomasella, L.; Turatto, M.

    2012-11-01

    We report that optical spectroscopy (range 340-820 nm; resolution 1.3 nm) obtained on Nov. 8.18 UT with the Asiago 1.82-m Copernico Telescope (+ AFOSC), shows that PSN J13144705+5405055 (= MASTER OT J131447.05+540505.5) is a type-Ia SN at a redshift z~0.033 (Sloan Digital Sky Survey Data Release 3). The best match was found with type-Ia SN 2002bo (Benetti et al. 2004, MNRAS, 348, 261) about 3-4 weeks after the B band maximum light.

  17. Complete Genome Sequence of the Larvicidal Bacterium Lysinibacillus sphaericus Strain OT4b.25

    PubMed Central

    Rey, Andrés; Silva-Quintero, Laura

    2016-01-01

    Lysinibacillus sphaericus OT4b.25 is a native Colombian strain isolated from coleopteran larvae in an oak forest near Bogotá D.C.; this strain has shown high levels of pathogenic activity against Culex quinquefasciatus larvae in laboratory assays compared to that of other members of the same species. Using Pacific Biosciences sequencing technology, we propose a chromosomal contig of 4,665,775 bp that, according to comparative analysis, is highly similar to that of reference strain L. sphaericus C3-41. PMID:27151786

  18. Synthesis and structure of [Na11(OtBu)10(OH)]: 1H NMR shift of a hydroxide ion encapsulated in a 21-vertex alcoholate cage.

    PubMed

    Geier, Jens; Grützmacher, Hansjörg

    2003-12-01

    [Na11(OtBu)10(OH)], a hydroxide enclosing 21-vertex cage compound, was found to crystallize from mixtures of sodium tert.butanolate with sodium hydroxide. Its structure can be derived from the known (NaOtBu)6-hexaprismane by replacing one butanolate unit with OH- and capping the latter with five additional units of NaOtBu. The hydroxide shows a signal at -3.21 ppm in the 1H NMR spectrum.

  19. Solar-energy-system performance evaluation: Honeywell OTS 44, Ocmulgee, Georgia

    NASA Astrophysics Data System (ADS)

    Mathur, A. K.; Pederson, S.

    1982-08-01

    The operation and technical performance of the solar operational test site (OTS 44) are described, based on data collected between April, 1981 and August, 1981. The following topics are discussed: system description, performance assessment, operating energy, energy savings, system maintenance, and conclusions. The solar energy system at OTS 44 is a hydronic heating and cooling system consisting of 5040 square feet of liquid cooled flat plate collectors; a 4000 gallon thermal storage tank; one 25 ton capacity organic Rankine cycle engine assisted water chillers; a forced draft cooling tower; and associated piping, pumps, valves, controls and heat rejection equipment. The solar system has eight basic modes of operation and several combination modes for providing space conditioning and hot water to the building. Data monitored during the 4 months of the operational test period found that the solar system collected 285 MMBtu of thermal energy of the total incident solar energy of 1040 MMBtu and provided 210 MMBtu for cooling and 10 MMBtu for heating and hot water. The net electrical energy saving due to the solar system was approximately 2600 kWh(e), and fossil energy saving was about 20 million Btu (MMBtu).

  20. Solar energy system performance evaluation: Honeywell OTS 41, Shenandoah (Newman), Georgia

    NASA Astrophysics Data System (ADS)

    Mathur, A. K.; Pederson, S.

    1982-08-01

    The operation and technical performance of the Solar Operational Test Site (OTS 41) located at Shenandoah, Georgia, are described, based on the analysis of the data collected between January and August 1981. The following topics are discussed: system description, performance assessment, operating energy, energy savings, system maintenance, and conclusions. The solar energy system at OTS 41 is a hydronic heating and cooling system consisting of 702 square feet of liquid-cooled flat-plate collectors; a 1000-gallon thermal storage tank; a 3-ton capacity organic Rankine-cycle-engine-assisted air conditioner; a water-to-are heat exchanger for solar space heating; a finned-tube coil immersed in the storage tank to preheat water for a gas-fired hot water heater; and associated piping, pumps, valves, and controls. The solar system has six basic modes of operation and several combination modes. The system operation is controlled automatically by a Honeywell-designed microprocessor-based control system, which also provides diagnostics.

  1. SpOT the Correct Tissue Every Time in Multi-Tissue Blocks

    PubMed Central

    Coffey, Anna H.; Berry, Deborah L.; Johnson, Michael D.

    2015-01-01

    Multi-tissue paraffin blocks provide high throughput analysis with increased efficiency, experimental uniformity, and reduced time and cost. Tissue microarrays make up the majority of multi-tissue paraffin blocks, but increasingly, researchers are using non-arrayed blocks containing larger tissues from multiple individuals which can provide many of the advantages of tissue microarrays without substantial investment in planning and equipment. A critical component of any multi-tissue analysis is the orientation method used to identify each individual tissue. Although methods exist to maintain proper orientation and identification of tissues in multi-tissue blocks, most are not well-suited to non-arrayed blocks, may consume valuable space within an array and/or are difficult to produce in the standard histology laboratory. The Specimen Orientation Tag (SpOT) is a simple, low cost orientation tool that is clearly visible in paraffin blocks and all tissue sections for reliable specimen identification in arrayed and non-arrayed layouts. The SpOT provides advantages over existing orientation methods for non-arrayed blocks as it does not require any direct modification to the tissue and allows for flexibility in the arrangement of tissue pieces. PMID:26067587

  2. Solar-energy-system performance evaluation: Honeywell OTS 44, Ocmulgee, Georgia

    NASA Technical Reports Server (NTRS)

    Mathur, A. K.; Pederson, S.

    1982-01-01

    The operation and technical performance of the solar operational test site (OTS 44) are described, based on data collected between April, 1981 and August, 1981. The following topics are discussed: system description, performance assessment, operating energy, energy savings, system maintenance, and conclusions. The solar energy system at OTS 44 is a hydronic heating and cooling system consisting of 5040 square feet of liquid cooled flat plate collectors; a 4000 gallon thermal storage tank; one 25 ton capacity organic Rankine cycle engine assisted water chillers; a forced draft cooling tower; and associated piping, pumps, valves, controls and heat rejection equipment. The solar system has eight basic modes of operation and several combination modes for providing space conditioning and hot water to the building. Data monitored during the 4 months of the operational test period found that the solar system collected 285 MMBtu of thermal energy of the total incident solar energy of 1040 MMBtu and provided 210 MMBtu for cooling and 10 MMBtu for heating and hot water. The net electrical energy saving due to the solar system was approximately 2600 kWh(e), and fossil energy saving was about 20 million Btu (MMBtu).

  3. Accuracy of Conventional PCR Targeting the 16S rRNA Gene with the Ot-16sRF1 and Ot-16sRR1 Primers for Diagnosis of Scrub Typhus: a Case-Control Study.

    PubMed

    Kim, Choon-Mee; Cho, Min Keun; Kim, Dong-Min; Yun, Na-Ra; Kim, Seok Won; Jang, Sook Jin; Ahn, Young-Joon; Lim, Donghoon

    2016-01-01

    We retrospectively evaluated the accuracy of conventional PCR targeting the 16S rRNA gene (16S C-PCR) using the Ot-16sRF1/Ot-16sRR1 primers for diagnosing scrub typhus. The diagnosis of Orientia tsutsugamushi infection by 16S C-PCR presented an increased sensitivity of 87.0% and specificity of 100% compared with those obtained with other targets and is thus a simple and clinically useful method with good diagnostic accuracy.

  4. Genome sequence and description of the heavy metal tolerant bacterium Lysinibacillus sphaericus strain OT4b.31

    PubMed Central

    Peña-Montenegro, Tito David; Dussán, Jenny

    2013-01-01

    Lysinibacillus sphaericus strain OT4b.31 is a native Colombian strain having no larvicidal activity against Culex quinquefasciatus and is widely applied in the bioremediation of heavy-metal polluted environments. Strain OT4b.31 was placed between DNA homology groups III and IV. By gap-filling and alignment steps, we propose a 4,096,672 bp chromosomal scaffold. The whole genome (consisting of 4,856,302 bp long, 94 contigs and 4,846 predicted protein-coding sequences) revealed differences in comparison to the L. sphaericus C3-41 genome, such as syntenial relationships, prophages and putative mosquitocidal toxins. Sphaericolysin B354, the coleopteran toxin Sip1A and heavy metal resistance clusters from nik, ars, czc, cop, chr, czr and cad operons were identified. Lysinibacillus sphaericus OT4b.31 has applications not only in bioremediation efforts, but also in the biological control of agricultural pests. PMID:24501644

  5. DAO spectroscopic classification of MASTER OT J140958.91+174549.4 as a core-collapse supernova.

    NASA Astrophysics Data System (ADS)

    Balam, D. D.; Graham, M. L.

    2016-04-01

    A spectrum was obtained on UT April 30.39 of MASTER OT J140958.91+174549.4 (CSS160427:140959+174549) using the 1.82-m Plaskett telescope (National Research Council of Canada) covering the range 365-710 nm (resolution 0.35 nm). Cross-correlation with a template library using SNID (Blondin & Tonry 2007, ApJ, 666, 1024) shows MASTER OT J140958.91+174549.4 to be a core-collapse supernova near maximum light.

  6. Activity and stability of yeast alcohol dehydrogenase (YADH) entrapped in aerosol OT reverse micelles.

    PubMed

    Sarcar, S; Jain, T K; Maitra, A

    1992-02-20

    The activity and stability of yeast alcohol dehydrogenase (YADH) entrapped in aerosol OT reverse micellar droplets have been investigated spectrophotometrically. Various physical parameters, e.g., water pool size, w(0), pH, and temperature, were optimized for YADH in water/AOT/isooctane reverse micelles. It was found that the enzyme exhibits maximum activity at w(0) = 28 and pH 8.1. It was more active in reverse micelles than in aqueous buffers at a particular temperature and was denatured at about 307 degrees C in both the systems. At a particular temperature YADH entrapped in reverse micelles was less stable than when it was dissolved in aqueous buffer.

  7. Entraining IDyOT: Timing in the Information Dynamics of Thinking

    PubMed Central

    Forth, Jamie; Agres, Kat; Purver, Matthew; Wiggins, Geraint A.

    2016-01-01

    We present a novel hypothetical account of entrainment in music and language, in context of the Information Dynamics of Thinking model, IDyOT. The extended model affords an alternative view of entrainment, and its companion term, pulse, from earlier accounts. The model is based on hierarchical, statistical prediction, modeling expectations of both what an event will be and when it will happen. As such, it constitutes a kind of predictive coding, with a particular novel hypothetical implementation. Here, we focus on the model's mechanism for predicting when a perceptual event will happen, given an existing sequence of past events, which may be musical or linguistic. We propose a range of tests to validate or falsify the model, at various different levels of abstraction, and argue that computational modeling in general, and this model in particular, can offer a means of providing limited but useful evidence for evolutionary hypotheses. PMID:27803682

  8. Bayesian reconstruction strategy of fluorescence-mediated tomography using an integrated SPECT-CT-OT system

    NASA Astrophysics Data System (ADS)

    Cao, Liji; Peter, Jörg

    2010-05-01

    Following the assembly of a triple-modality SPECT-CT-OT small animal imaging system providing intrinsically co-registered projection data of all three submodalities and under the assumption and investigation of dual-labeled probes consisting of both fluorophores and radionuclides, a novel multi-modal reconstruction strategy is presented in this paper aimed at improving fluorescence-mediated tomography (FMT). The following reconstruction procedure is proposed: firstly, standard x-ray CT image reconstruction is performed employing the FDK algorithm. Secondly, standard SPECT image reconstruction is performed using OSEM. Thirdly, from the reconstructed CT volume data the surface boundary of the imaged object is extracted for finite element definition. Finally, the reconstructed SPECT data are used as a priori information within a Bayesian reconstruction framework for optical (FMT) reconstruction. We provide results of this multi-modal approach using phantom experimental data and illustrate that this strategy does suppress artifacts and facilitates quantitative analysis for optical imaging studies.

  9. Television Microwave Link (TML) Operational Test and Evaluation (OT/E)/integration test report

    NASA Astrophysics Data System (ADS)

    Bell, Wayne E.; Tran, Tuan A.

    1992-03-01

    Given here are the results of the Operational Test and Evaluation (OT&E)/Integration test on the 15 GHz Television Microwave Link (TML) system. The TML is commercial off the shelf equipment. It provides the capability for air traffic control information to be relayed to a remote display from the Federal Aviation Administration (FAA) Digital Bright Radar Indicator Tower (DBRITE) system located at the terminal facility. The TML system was integrated and tested with the DBRITE system. Testing was successfully completed and met all of the T&E/Integration requirements. The acceptability of the remote DBRITE display was evaluated by the air traffic controllers and the data displayed on the remote DBRITE display provided the necessary information for air traffic control services. Therefore, the TML system is recommended for deployment.

  10. The crystal structure of Z-Gly-Aib-Gly-Aib-OtBu.

    PubMed

    Gessmann, Renate; Brückner, Hans; Aivaliotis, Michalis; Petratos, Kyriacos

    2015-06-01

    The synthetic peptide Z-Gly-Aib-Gly-Aib-OtBu was dissolved in methanol and crystallized in a mixture of ethyl acetate and petroleum ether. The crystals belong to the centrosymmetric space group P4/n that is observed less than 0.3% in the Cambridge Structural Database. The first Gly residue assumes a semi-extended conformation (φ ±62°, ψ ∓131°). The right-handed peptide folds in two consecutive β-turns of type II' and type I or an incipient 310 -helix, and the left-handed counterpart folds accordingly in the opposite configuration. In the crystal lattice, one molecule is linked to four neighbors in the ab-plane via hydrogen bonds. These bonds form a continuous network of left- and right-handed molecules. The successive ab-planes stack via apolar contacts in the c-direction. An ethyl acetate molecule is situated on and close to the fourfold axis.

  11. Cloning, purification, crystallization and preliminary crystallographic analysis of acylphosphatase from Pyrococcus horikoshii OT3.

    PubMed

    Miyazono, Ken Ichi; Kudo, Norio; Tanokura, Masaru

    2004-06-01

    Acylphosphatase is one of the smallest enzymes and catalyzes the hydrolysis of the carboxy-phosphate bond. An extremely thermostable acylphosphatase from a hyperthermophilic archaea, Pyrococcus horikoshii OT3, has been cloned, expressed in Escherichia coli, purified and crystallized using the sitting-drop vapour-diffusion method with potassium/sodium tartrate as the precipitant at pH 5.5. X-ray diffraction data have been collected to a highest resolution of 1.72 angstroms on a synchrotron-radiation source. The crystals belong to space group P3(2)21, with approximate unit-cell parameters a = b = 86.6, c = 75.4 angstroms and two monomers in the asymmetric unit.

  12. Solar energy system performance evaluation: final report for Honeywell OTS 41, Shenandoah (Newnan), Georgia

    SciTech Connect

    Mathur, A K; Pederson, S

    1982-08-01

    The operation and technical performance of the Solar Operational Test Site (OTS 41) located at Shenandoah, Georgia, are described, based on the analysis of data collected between January and August 1981. The following topics are discussed: system description, performance assessment, operating energy, energy savings, system maintenance, and conclusions. The solar energy system at OTS 41 is a hydronic heating and cooling system consisting of 702 square feet of liquid-cooled flat-plate collectors; a 1000-gallon thermal storage tank; a 3-ton capacity organic Rankine-cycle-engine-assisted air conditioner; a water-to-air heat exchanger for solar space heating; a finned-tube coil immersed in the storage tank to preheat water for a gas-fired hot water heater; and associated piping, pumps, valves, and controls. The solar system has six basic modes of operation and several combination modes. The system operation is controlled automatically by a Honeywell-designed microprocessor-based control system, which also provides diagnostics. Based on the instrumented test data monitored and collected during the 7 months of the Operational Test Period, the solar system collected 53 MMBtu of thermal energy of the total incident solar energy of 219 MMBtu and provided 11.4 MMBtu for cooling, 8.6 MMBtu for heating, and 8.1 MMBtu for domestic hot water. The projected net annual energy savings due to the solar system were approximately 50 MMBtu of fossil energy (49,300 cubic feet of natural gas) and a loss of 280 kWh(e) of electrical energy.

  13. The Persistent Eruption of UGC 2773-OT: finally, a decade-long extragalactic Eta Carinae analogue

    NASA Astrophysics Data System (ADS)

    Smith, Nathan; Andrews, Jennifer E.; Mauerhan, Jon C.; Zheng, WeiKang; Filippenko, Alexei V.; Graham, Melissa L.; Milne, Peter

    2016-02-01

    While supernova (SN) impostors resemble the Great Eruption of η Carinae in the sense that their spectra show narrow H lines and they have typical peak absolute magnitudes of -13 to -14 mag, most extragalactic events observed so far are quite different from η Car in duration. Their bright phases typically last for ˜100 d or less, rather than persisting for several years. The transient object UGC 2773-OT (discovered in 2009) had a similar peak absolute magnitude to other SN impostors, but with a gradual 5-yr pre-discovery rise. In the ˜6 yr since discovery, it has faded very slowly (0.26 mag yr-1). Overall, we suggest that its decade-long eruption is so far the best-known analogue of η Car's 19th century eruption. We discuss extensive spectroscopy of the ongoing eruption. The spectra show interesting changes in velocity and line shape that we discuss in detail, including an asymmetric Hα emission line that we show is consistent with the ejection of a bipolar nebula that could be very much like the Homunculus of η Car. Moreover, changes in the line width, line profile, blue excess emission resembling that of Type IIn SNe, and the intensity of Hα suggest the presence of strong circumstellar interaction in the eruption at late times. This supports the hypothesis that the extended plateau of η Car's eruption may have been powered by shock interaction as well. One interesting difference compared to η Car, however, is that UGC 2773-OT so far does not exhibit the repeated brief spikes in luminosity that have been associated with binary periastron events.

  14. Spectroscopic confirmation of MASTER OT J004514.13+420007.2 as a Classical Nova eruption in M31

    NASA Astrophysics Data System (ADS)

    Darnley, M. J.; Williams, S. C.

    2016-01-01

    We obtained a spectrum of the classical nova candidate MASTER OT J004514.13+420007.2 (ATel #8603, #8606) with the low-resolution (R~350) SPRAT spectrograph on the 2m Liverpool Telescope (Steele et al. 2004) on 2016 January 30.82 UT (2.4 days post-discovery; ATel #8606).

  15. Emerging role of long non-coding RNA SOX2OT in SOX2 regulation in breast cancer.

    PubMed

    Askarian-Amiri, Marjan E; Seyfoddin, Vahid; Smart, Chanel E; Wang, Jingli; Kim, Ji Eun; Hansji, Herah; Baguley, Bruce C; Finlay, Graeme J; Leung, Euphemia Y

    2014-01-01

    The transcription factor SOX2 is essential for maintaining pluripotency in a variety of stem cells. It has important functions during embryonic development, is involved in cancer stem cell maintenance, and is often deregulated in cancer. The mechanism of SOX2 regulation has yet to be clarified, but the SOX2 gene lies in an intron of a long multi-exon non-coding RNA called SOX2 overlapping transcript (SOX2OT). Here, we show that the expression of SOX2 and SOX2OT is concordant in breast cancer, differentially expressed in estrogen receptor positive and negative breast cancer samples and that both are up-regulated in suspension culture conditions that favor growth of stem cell phenotypes. Importantly, ectopic expression of SOX2OT led to an almost 20-fold increase in SOX2 expression, together with a reduced proliferation and increased breast cancer cell anchorage-independent growth. We propose that SOX2OT plays a key role in the induction and/or maintenance of SOX2 expression in breast cancer.

  16. Emerging Role of Long Non-Coding RNA SOX2OT in SOX2 Regulation in Breast Cancer

    PubMed Central

    Askarian-Amiri, Marjan E.; Seyfoddin, Vahid; Smart, Chanel E.; Wang, Jingli; Kim, Ji Eun; Hansji, Herah; Baguley, Bruce C.; Finlay, Graeme J.; Leung, Euphemia Y.

    2014-01-01

    The transcription factor SOX2 is essential for maintaining pluripotency in a variety of stem cells. It has important functions during embryonic development, is involved in cancer stem cell maintenance, and is often deregulated in cancer. The mechanism of SOX2 regulation has yet to be clarified, but the SOX2 gene lies in an intron of a long multi-exon non-coding RNA called SOX2 overlapping transcript (SOX2OT). Here, we show that the expression of SOX2 and SOX2OT is concordant in breast cancer, differentially expressed in estrogen receptor positive and negative breast cancer samples and that both are up-regulated in suspension culture conditions that favor growth of stem cell phenotypes. Importantly, ectopic expression of SOX2OT led to an almost 20-fold increase in SOX2 expression, together with a reduced proliferation and increased breast cancer cell anchorage-independent growth. We propose that SOX2OT plays a key role in the induction and/or maintenance of SOX2 expression in breast cancer. PMID:25006803

  17. 12 CFR 563b.515 - What information must I provide to OTS before I repurchase my shares?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... repurchase my shares? 563b.515 Section 563b.515 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF... What information must I provide to OTS before I repurchase my shares? (a) To repurchase stock in the first year following conversion, other than repurchases under § 563b.510(a)(3) or (a)(4), you must...

  18. 12 CFR 563b.515 - What information must I provide to OTS before I repurchase my shares?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... repurchase my shares? 563b.515 Section 563b.515 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF... What information must I provide to OTS before I repurchase my shares? (a) To repurchase stock in the first year following conversion, other than repurchases under § 563b.510(a)(3) or (a)(4), you must...

  19. 12 CFR 502.29 - How does OTS determine the condition component for a savings and loan holding company?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... holding company structure) is a composite rating of 4 or 5, OTS will assess a charge under the condition... for a savings and loan holding company? 502.29 Section 502.29 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY ASSESSMENTS AND FEES Assessments Savings and Loan Holding...

  20. 12 CFR 502.26 - How does OTS calculate the semi-annual assessment for savings and loan holding companies?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... reflect OTS costs of supervising the holding company structure and: (i) There are multiple top-tier holding companies in the holding company structure; (ii) The top-tier holding company is organized outside...) Other circumstances indicate that the assessment of the top-tier holding company is inappropriate....

  1. 12 CFR 502.29 - How does OTS determine the condition component for a savings and loan holding company?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... holding company? (a) If the most recent examination rating assigned to the responsible savings and loan holding company (or most recent examination rating assigned to any savings and loan holding company in the... system. OTS uses the most recent rating of which the savings and loan holding company has been...

  2. 75 FR 63107 - Alternatives to the Use of External Credit Ratings in the Regulations of the OTS

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-14

    ... capital frameworks. (75 FR 52283) II. OTS Regulations Referencing Credit Ratings The non-capital... different regulations? \\8\\ 75 FR 52283, August 25, 2010. Alternatives for Replacing References to Credit... banking agencies), including alternative measures of credit-worthiness that may be used in lieu of...

  3. Theileria sp. OT3 and other tick-borne pathogens in sheep and ticks in Italy: molecular characterization and phylogeny.

    PubMed

    Giangaspero, A; Marangi, M; Papini, R; Paoletti, B; Wijnveld, M; Jongejan, F

    2015-02-01

    PCR Reverse Line Blot (RLB) hybridization and sequencing were used to determine the dynamics of infection with tick-borne pathogens in one hundred apparently healthy sheep in Italy. Blood samples were tested once prior to the onset of the grazing season (June 2010) and once after the end of the grazing season (August 2010). Ticks collected from sheep and from the vegetation were also tested by PCR/RLB. Before grazing, 56% of the sheep harbored several tick-borne pathogens: Anaplasma ovis was the most prevalent (41%), followed by A. ovis co-infected with Theileria sp. OT3 (14%). After grazing, 87% of sheep were positive for A. ovis alone (41%), co-infected with Theileria sp. OT3 (8%) or co-infected with Babesia motasi (5%). Other sheep were infected with Anaplasma phagocytophilum alone (20%), co-infected with B. motasi (7%) or with Theileria sp. OT3 (5%) (p<0.001). After grazing, sheep were significantly more infected with tick-borne pathogens than before grazing. Ticks collected were all Haemaphysalis punctata (n-89) and 36% were positive for A. ovis, Ehrlichia ovina and A. ovis combined with A. phagocytophilum. Phylogenetic analysis including isolates from countries in the Mediterranean Basin show circulation of the same variants of Theileria sp. OT3, whereas two different geographical origins for the isolates of A. ovis and A. phagocytophilum were identified. This is the first report from Italy of Theileria sp. OT3 in sheep, whereas the detection of Ehrlichia ovina in ticks is worth noting, and the presence of A. phagocytophilum in sheep and in ticks poses a potential public health risk. PMID:25448422

  4. Isolation and Pharmacological Characterization of α-Elapitoxin-Ot1a, a Short-Chain Postsynaptic Neurotoxin from the Venom of the Western Desert Taipan, Oxyuranus temporalis

    PubMed Central

    Barber, Carmel M.; Ahmad Rusmili, Muhamad Rusdi; Hodgson, Wayne C.

    2016-01-01

    Taipans (Oxyuranus spp.) are elapids with highly potent venoms containing presynaptic (β) and postsynaptic (α) neurotoxins. O. temporalis (Western Desert taipan), a newly discovered member of this genus, has been shown to possess venom which displays marked in vitro neurotoxicity. No components have been isolated from this venom. We describe the characterization of α-elapitoxin-Ot1a (α-EPTX-Ot1a; 6712 Da), a short-chain postsynaptic neurotoxin, which accounts for approximately 30% of O. temporalis venom. α-Elapitoxin-Ot1a (0.1–1 µM) produced concentration-dependent inhibition of indirect-twitches, and abolished contractile responses to exogenous acetylcholine and carbachol, in the chick biventer cervicis nerve-muscle preparation. The inhibition of indirect twitches by α-elapitoxin-Ot1a (1 µM) was not reversed by washing the tissue. Prior addition of taipan antivenom (10 U/mL) delayed the neurotoxic effects of α-elapitoxin-Ot1a (1 µM) and markedly attenuated the neurotoxic effects of α-elapitoxin-Ot1a (0.1 µM). α-Elapitoxin-Ot1a displayed pseudo-irreversible antagonism of concentration-response curves to carbachol with a pA2 value of 8.02 ± 0.05. De novo sequencing revealed the main sequence of the short-chain postsynaptic neurotoxin (i.e., α-elapitoxin-Ot1a) as well as three other isoforms found in O. temporalis venom. α-Elapitoxin-Ot1a shows high sequence similarity (i.e., >87%) with other taipan short-chain postsynaptic neurotoxins. PMID:26938558

  5. Isolation and Pharmacological Characterization of α-Elapitoxin-Ot1a, a Short-Chain Postsynaptic Neurotoxin from the Venom of the Western Desert Taipan, Oxyuranus temporalis.

    PubMed

    Barber, Carmel M; Rusmili, Muhamad Rusdi Ahmad; Hodgson, Wayne C

    2016-03-01

    Taipans (Oxyuranus spp.) are elapids with highly potent venoms containing presynaptic (β) and postsynaptic (α) neurotoxins. O. temporalis (Western Desert taipan), a newly discovered member of this genus, has been shown to possess venom which displays marked in vitro neurotoxicity. No components have been isolated from this venom. We describe the characterization of α-elapitoxin-Ot1a (α-EPTX-Ot1a; 6712 Da), a short-chain postsynaptic neurotoxin, which accounts for approximately 30% of O. temporalis venom. α-Elapitoxin-Ot1a (0.1-1 µM) produced concentration-dependent inhibition of indirect-twitches, and abolished contractile responses to exogenous acetylcholine and carbachol, in the chick biventer cervicis nerve-muscle preparation. The inhibition of indirect twitches by α-elapitoxin-Ot1a (1 µM) was not reversed by washing the tissue. Prior addition of taipan antivenom (10 U/mL) delayed the neurotoxic effects of α-elapitoxin-Ot1a (1 µM) and markedly attenuated the neurotoxic effects of α-elapitoxin-Ot1a (0.1 µM). α-Elapitoxin-Ot1a displayed pseudo-irreversible antagonism of concentration-response curves to carbachol with a pA₂ value of 8.02 ± 0.05. De novo sequencing revealed the main sequence of the short-chain postsynaptic neurotoxin (i.e., α-elapitoxin-Ot1a) as well as three other isoforms found in O. temporalis venom. α-Elapitoxin-Ot1a shows high sequence similarity (i.e., >87%) with other taipan short-chain postsynaptic neurotoxins. PMID:26938558

  6. Effect of Ozone Therapy (OT) on Healing of Colonic Anastomosis in a Rat Model of Peritonitis

    PubMed Central

    Erginel, Başak; Erginel, Turgay; Aksoy, Bilgin; Dokucu, Ali İhsan

    2014-01-01

    Background: Ozone is a three-oxygen molecule (O3). Ozone therapy (OT) is systematically effective when pathological inflammatory and immunologic processes are activated. Among of these conditions are wound healing, macular degeneration related to aging, and conditions that are ischemic or infectious. Aims: The aim of this study was to determine the effects of OT on wound healing of intestinal anastomosis in the presence of peritonitis in a rat model. Study Design: Animal experimentation. Methods: A total of 40 Wistar albino rats were randomized into four groups (n=10) including: sham (S), peritonitis (P), ozone 0 (O0), and ozone 24 (O24). In group S, only cecal dissection was carried out. The S group had only a cecal dissection and intestinal anastomosis performed, but no peritonitis. In all other groups, cecal ligation and puncture (CLP) followed the cecal dissection to induce bacterial peritonitis. 24 h after puncture, a cecal resection and ileocolic anastomosis were performed. In group P, 24 h after CLP, a cecal resection and ileocolic anastomosis were performed and no ozone was administered. In group O0, immediately after the anastomosis, and in group O24, starting 24 hours after the anastomosis, an intraperitoneal 1 mg/kg/day ozone administration was applied for seven days. On the seventh day the animals were sacrificed, the anastomotic bursting pressures (BP) and the hydroxyproline values of the anastomotic tissues were measured, and histopathologic examination of the anastomotic segment was carried out. Results: The highest BP was in group S, with 211±23.13 mmHg. The mean BP of group P was 141±56.25 mmHg, which was significantly lower than in the other two peritonitis groups that received ozone therapy, group O0 and O24, where it was 192±22 and 166±45 mmHg, respectively (p<0.05). The difference in the BP between groups O0 and O24 was not statistically significant (p>0.05). Histopathologic analyses of the anastomotic segments determined there was

  7. Almost gone: SN 2008S and NGC 300 2008OT-1 are fainter than their progenitors

    NASA Astrophysics Data System (ADS)

    Adams, S. M.; Kochanek, C. S.; Prieto, J. L.; Dai, X.; Shappee, B. J.; Stanek, K. Z.

    2016-08-01

    We present late-time Hubble and Spitzer Space Telescope (SST) imaging of SN 2008S and NGC 300 2008OT-1, the prototypes of a common class of stellar transients whose true nature is debated. Both objects are still fading and are now >15 times fainter than their progenitors in the mid-IR and are undetected in the optical and near-IR. Data from the Large Binocular Telescope and Magellan show that neither source has been variable in the optical since fading in 2010. We present models of surviving sources obscured by dusty shells or winds and find that extreme dust models are needed for surviving stars to be successfully hidden by dust, which suggests that these transients may be genuine, but low-energy, supernova explosions. Though SN 2008S is not detected in Chandra X-Ray Observatory data taken in 2012, the flux limits allow the fading IR source to be powered solely by the shock interaction of ejecta with the circumstellar medium if the shock velocity at the time of the observation was ≳20 per cent slower than estimated from emission line widths while the transient was still optically bright. Continued SST monitoring and 10-20 μm observations with James Webb Space Telescope can resolve any remaining ambiguities.

  8. Rotational reorientation dynamics of Aerosol-OT reverse micelles formed in near-critical propane

    SciTech Connect

    Heitz, M.P.; Bright, F.V.

    1996-06-01

    The rotational reorientation kinetics of two fluorescent solutes (rhodamine 6G, R6G, and rhodamine 101, R101) have been determined in sodium bis(2-ethylhexyl) sulfosuccinate (Aerosol-OT, AOT) reverse micelles formed in liquid and near-critical propane. We show that the amount of water loading ([water]/[AOT], R), continuous phase density, and temperature all influence the solute rotational dynamics. In all cases, the decay of anisotropy data (i.e., frequency-dependent differential polarized phase angle and polarized modulation ratio) are well described by a bi-exponential decay law. We find that the faster rotational correlation times are similar to but slightly less than the values predicted for an individual AOT reverse micelle rotating in propane. The recovered rotational correlation times range from 200 to 500 ps depending on experimental conditions. This faster rotational process is explained in terms of lateral diffusion of the fluorophore along the water/headgroup interfacial region within the reverse micelle. The recovered values for the slower rotational correlation times range from 7 to 18 ns. These larger rotational reorientation times are assigned to varying micelle-micelle (i.e., tail-tail) interactions in the low-density, highly compressible fluid region. We also quantify the contribution of the reverse micellar {open_quotes}aggregate{close_quotes} to the total decay of anisotropy. {copyright} {ital 1996} {ital Society for Applied Spectroscopy}

  9. MASTER OT J130845.02-323254.9: Variable Stars as Source of the High Energy Neutrino.

    NASA Astrophysics Data System (ADS)

    Lipunov, V.; Tyurina, N.; Gorbovskoy, E.; Buckley, D.

    2016-09-01

    As reported in ATel #9425 Global MASTER Net auto-detection system ( ( Lipunov et al., MASTER Global Robotic Net, Advances in Astronomy, 2010, 30L) discovered OT source at (RA, Dec) = 13h 08m 45.02s -32d 32m 54.9s on 2016-08-24.73811 UT during inspection of HESE IceCube alert (14 August 2016, 58537957 trigger number http://gcn.gsfc.nasa.gov/notices_amon/58537957_128340.amon , Dornic et al. ATEL #9440 ). MASTER-SAAO auto-detection system detected again OT at RA (2000) = 13 08 45.02 -32 32 54.9 on 2016-09-04.7627UT (ATEL #9425).

  10. FLOYDS Classification of MASTER OT J164244.87+272054.3 as a Type Ia Supernova Near Peak

    NASA Astrophysics Data System (ADS)

    Hosseinzadeh, G.; Arcavi, I.; Howell, D. A.; McCully, C.; Valenti, S.

    2016-05-01

    We obtained a spectrum of MASTER OT J164244.87+272054.3 (ATel #9022) on 2016 May 6.6 UT with the robotic FLOYDS instrument mounted on the LCOGT 2-meter telescope in Siding Spring, Australia. Using SNID (Blondin & Tonry 2007, ApJ, 666, 1024), we find a good fit to the normal Type Ia SN 1990N 2 days after maximum light at redshift z=0.108.

  11. Limited Production (LP) Precision Runway Monitor (PRM) Operational Test and Evaluation integration and OT and E Operational Test Plan

    NASA Astrophysics Data System (ADS)

    Livings, Jeffrey

    1995-05-01

    This document defines the Test Plan and corresponding Test Verification Requirements Traceability Matrix (TVRTM) that will be used to conduct the Limited Production (LP) Precision Runway Monitor (PRM) Operational Test and Evaluation (OT and E) Integration and OT and E Operational tests. These tests will be conducted at the Minneapolis-St. Paul International Airport following the Contractor Site Acceptance Test. The LP PRM OT and E test effort will concentrate on Operational Effectiveness and Suitability. The Operational Effectiveness Test consists of a review of the contractor performed Development Test and Evaluation (DT and E) and Site Acceptance Tests. This review will evaluate whether each of the Measures of Effectiveness had been satisfactorily tested and whether the results meet the Minimum Acceptable Operational REquirements MAORs). This review will be conducted solely by test engineers and does not require the PRM system. The Operational Suitability Tests will expose the test participants (Air Traffic (AT) Controllers and Airway Facilities (AF) Technicians) to the PRM system in an operational environment while they perform specified operational procedures. These tests will be conducted in two separate phases: AT Suitability and AF Suitability. Each of these phases is focused on the specific test participants.

  12. Rice OVERLY TOLERANT TO SALT 1 (OTS1) SUMO protease is a positive regulator of seed germination and root development.

    PubMed

    Srivastava, Anjil Kumar; Zhang, Cunjin; Sadanandom, Ari

    2016-05-01

    Salinity is one of the major environmental stresses affecting rice production worldwide. Improving rice salt tolerance is a critical step for sustainable food production. Posttranslational modifications of proteins greatly expand proteome diversity, increase functionality and allow quick responses to environmental stresses, all at low cost to the cell. SUMO mediated modification of substrate proteins is a highly dynamic process governed by the balance of activities of SUMO E3 ligases and deconjugating SUMO proteases. In recent years, SUMO (Small Ubiquitin like Modifier) conjugation of proteins has emerged as an influential regulator of stress signaling in the model plant Arabidopsis. However SUMOylation remain largely under studied in crop plants. We recently identified the SUMO protease gene family in rice and demonstrated a role for OsOTS1 SUMO proteases in salt stress. Interestingly, rice plants silencing OsOTS1 also show significantly reduced germination rate. Knockdown of OsOTS1 gene expression affects root growth by primarily reducing cell size rather than cell division. PMID:27119209

  13. Rice OVERLY TOLERANT TO SALT 1 (OTS1) SUMO protease is a positive regulator of seed germination and root development.

    PubMed

    Srivastava, Anjil Kumar; Zhang, Cunjin; Sadanandom, Ari

    2016-05-01

    Salinity is one of the major environmental stresses affecting rice production worldwide. Improving rice salt tolerance is a critical step for sustainable food production. Posttranslational modifications of proteins greatly expand proteome diversity, increase functionality and allow quick responses to environmental stresses, all at low cost to the cell. SUMO mediated modification of substrate proteins is a highly dynamic process governed by the balance of activities of SUMO E3 ligases and deconjugating SUMO proteases. In recent years, SUMO (Small Ubiquitin like Modifier) conjugation of proteins has emerged as an influential regulator of stress signaling in the model plant Arabidopsis. However SUMOylation remain largely under studied in crop plants. We recently identified the SUMO protease gene family in rice and demonstrated a role for OsOTS1 SUMO proteases in salt stress. Interestingly, rice plants silencing OsOTS1 also show significantly reduced germination rate. Knockdown of OsOTS1 gene expression affects root growth by primarily reducing cell size rather than cell division.

  14. Impaired Memory in OT-II Transgenic Mice Is Associated with Decreased Adult Hippocampal Neurogenesis Possibly Induced by Alteration in Th2 Cytokine Levels

    PubMed Central

    Jeon, Seong Gak; Kim, Kyoung Ah; Chung, Hyunju; Choi, Junghyun; Song, Eun Ji; Han, Seung-Yun; Oh, Myung Sook; Park, Jong Hwan; Kim, Jin-il; Moon, Minho

    2016-01-01

    Recently, an increasing number of studies have focused on the effects of CD4+ T cell on cognitive function. However, the changes of Th2 cytokines in restricted CD4+ T cell receptor (TCR) repertoire model and their effects on the adult hippocampal neurogenesis and memory are not fully understood. Here, we investigated whether and how the mice with restricted CD4+ repertoire TCR exhibit learning and memory impairment by using OT-II mice. OT-II mice showed decreased adult neurogenesis in hippocampus and short- and long- term memory impairment. Moreover, Th2 cytokines in OT-II mice are significantly increased in peripheral organs and IL-4 is significantly increased in brain. Finally, IL-4 treatment significantly inhibited the proliferation of cultured adult rat hippocampal neural stem cells. Taken together, abnormal level of Th2 cytokines can lead memory dysfunction via impaired adult neurogenesis in OT-II transgenic. PMID:27432189

  15. Impaired Memory in OT-II Transgenic Mice Is Associated with Decreased Adult Hippocampal Neurogenesis Possibly Induced by Alteration in Th2 Cytokine Levels.

    PubMed

    Jeon, Seong Gak; Kim, Kyoung Ah; Chung, Hyunju; Choi, Junghyun; Song, Eun Ji; Han, Seung-Yun; Oh, Myung Sook; Park, Jong Hwan; Kim, Jin-Il; Moon, Minho

    2016-08-31

    Recently, an increasing number of studies have focused on the effects of CD4+ T cell on cognitive function. However, the changes of Th2 cytokines in restricted CD4+ T cell receptor (TCR) repertoire model and their effects on the adult hippocampal neurogenesis and memory are not fully understood. Here, we investigated whether and how the mice with restricted CD4+ repertoire TCR exhibit learning and memory impairment by using OT-II mice. OT-II mice showed decreased adult neurogenesis in hippocampus and short- and long- term memory impairment. Moreover, Th2 cytokines in OT-II mice are significantly increased in peripheral organs and IL-4 is significantly increased in brain. Finally, IL-4 treatment significantly inhibited the proliferation of cultured adult rat hippocampal neural stem cells. Taken together, abnormal level of Th2 cytokines can lead memory dysfunction via impaired adult neurogenesis in OT-II transgenic. PMID:27432189

  16. Crystal Structural and Functional Analysis of the Putative Dipeptidase from Pyrococcus horikoshii OT3.

    PubMed

    Jeyakanthan, Jeyaraman; Takada, Katsumi; Sawano, Masahide; Ogasahara, Kyoko; Mizutani, Hisashi; Kunishima, Naoki; Yokoyama, Shigeyuki; Yutani, Katsuhide

    2009-01-01

    The crystal structure of a putative dipeptidase (Phdpd) from Pyrococcus horikoshii OT3 was solved using X-ray data at 2.4 A resolution. The protein is folded into two distinct entities. The N-terminal domain consists of the general topology of the alpha/beta fold, and the C-terminal domain consists of five long mixed strands, four helices, and two 3(10) helices. The structure of Phdpd is quite similar to reported structures of prolidases from P. furiosus (Zn-Pfprol) and P. horikoshii (Zn-Phdpd), where Zn ions are observed in the active site resulting in an inactive form. However, Phdpd did not contain metals in the crystal structure and showed prolidase activity in the absence of additional Co ions, whereas the specific activities increased by 5 times in the presence of a sufficient concentration (1.2 mM) of Co ions. The substrate specificities (X-Pro) of Phdpd were broad compared with those of Zn-Phdpd in the presence of Co ions, whose relative activities are 10% or less for substrates other than Met-Pro, which is the most favorable substrate. The binding constants of Zn-Phdpd with three metals (Zn, Co, and Mn) were higher than those of Phdpd and that with Zn was higher by greater than 2 orders, which were determined by DSC experiments. From the structural comparison of both forms and the above experimental results, it could be elucidated why the protein with Zn(2+) ions is inactive.

  17. OT2_dpadgett_2: Warm A Star Debris Disks from WISE

    NASA Astrophysics Data System (ADS)

    Padgett, D.

    2011-09-01

    Debris disks trace the collisional breakdown of asteroid and comet parent bodies orbiting nearby main sequence stars. Debris disks are typically cold analogs of our Kuiper belt with emission peaking near 70 microns wavelength. However, a relatively small number of warm disks are known with emission at 22 - 24 microns. These systems are especially interesting because they trace dust in the region likely to host terrestrial planets, where the dust has a short dynamical lifetimes. They also tend to be young systems aged < 1 Gyr. This knowledge of warm debris disks - extrasolar analogs to our solar system's Zodiacal cloud - is based on the 25 year old IRAS survey and observations of selected targets with ISO and Spitzer. The Wide-Field Infrared Survey Explorer (WISE) has recently completed new, sensitive all-sky mapping in the 3.3, 4.6, 12, and 22 micron bands. Association of the WISE sources to Hipparcos and Tycho stars has led to the identification of 61 nearby main sequence A stars with robustly detected warm 22 micron excesses not previously known. To determine whether these systems represent outbursts of asteroidal dust production (such as in the HD 69830 system), or simply the Wien side of emission from a cold outer dust belt, photometry at longer wavelengths is needed. We propose Herschel/PACS 70 and 160 micron photometry of this unbiased sample of new A star debris disks. These data will allow us to fully characterize the dust temperature and infrared luminosity of these systems, allowing them to be understood in the context of other debris disks and disk evolution theory. Herschel OT1 observations of FGKM stars from this survey show a 90% detection rate for 70 micron excess emission. The results from these combined samples will strongly constrain our picture of the collisional history of inner planetary systems.

  18. Crystal structure of an archaeal Ski2p-like protein from Pyrococcus horikoshii OT3.

    PubMed

    Zhang, Xiaodong; Nakashima, Takashi; Kakuta, Yoshimitsu; Yao, Min; Tanaka, Isao; Kimura, Makoto

    2008-01-01

    The Ski complex composed of Ski2p, Ski3p, and Ski8p plays an essential role in the 3' to 5' cytoplasmic mRNA degradation pathway in yeast. Ski2p is a putative RNA helicase, belonging in the DExD/H-box protein families and conserved in eukarya as well as in archaea. The gene product (Ph1280p) from the hyperthermophilic archaeon Pyrococcus horikoshii OT3 shows sequence homology with Ski2p, sharing 22.6% identical amino acids with a central region of Ski2p. In order to gain structural information about the Ski2p-like RNA helicase, we overproduced Ph1280p in Escherichia coli cells, and purified it to apparent homogeneity. Ph1280p exhibits DNA/RNA-dependent ATPase activity with an optimal temperature at approximately 90 degrees C. The crystal structure of Ph1280p has been solved at a resolution of 3.5 A using single-wavelength anomalous dispersion (SAD) and selenomethionyl (Se-Met)-substituted protein. Ph1280p comprises four subdomains; the two N-terminal subdomains (N1 and N2) fold into an RecA-like architecture with the conserved helicase motifs, while the two C-terminal subdomains (C1 and C2) fold into alpha-helical structures containing a winged helix (WH)-fold and helix-hairpin-helix (HhH)-fold, respectively. Although the structure of each of the Ph1280p subdomains can be individually superimposed on the corresponding domains in other helicases, such as the Escherichia coli DNA helicase RecQ, the relative orientation of the helicase and C-terminal subdomains in Ph1280p is significantly different from that of other helicases. This structural feature is implicated in substrate specificity for the Ski2-like helicase and would play a critical role in the 3' to 5' cytoplasmic mRNA degradation in the Ski complex. PMID:18042682

  19. Characterization of the archaeal ribonuclease P proteins from Pyrococcus horikoshii OT3.

    PubMed

    Terada, Atsushi; Honda, Takashi; Fukuhara, Hideo; Hada, Kazumasa; Kimura, Makoto

    2006-08-01

    Ribonuclease P (RNase P) is a ribonucleoprotein complex involved in the processing of the 5'-leader sequence of precursor tRNA (pre-tRNA). Our earlier study revealed that RNase P RNA (pRNA) and five proteins (PhoPop5, PhoRpp38, PhoRpp21, PhoRpp29, and PhoRpp30) in the hyperthermophilic archaeon Pyrococcus horikoshii OT3 reconstituted RNase P activity that exhibits enzymatic properties like those of the authentic enzyme. In present study, we investigated involvement of the individual proteins in RNase P activity. Two particles (R-3Ps), in which pRNA was mixed with three proteins, PhoPop5, PhoRpp30, and PhoRpp38 or PhoPop5, PhoRpp30, and PhoRpp21 showed a detectable RNase P activity, and five reconstituted particles (R-4Ps) composed of pRNA and four proteins exhibited RNase P activity, albeit at reduced level compared to that of the reconstituted particle (R-5P) composed of pRNA and five proteins. Time-course analysis of the RNase P activities of R-4Ps indicated that the R-4Ps lacking PhoPop5, PhoRpp21, or PhoRpp30 had virtually reduced activity, while omission of PhoRpp29 or PhoRpp38 had a slight effect on the activity. The results indicate that the proteins contribute to RNase P activity in order of PhoPop5 > PhoRpp30 > PhoRpp21 > PhoRpp29 > PhoRpp38. It was further found that R-4Ps showed a characteristic Mg2+ ion dependency approximately identical to that of R-5P. However, R-4Ps had optimum temperature of around at 55 degrees C which is lower than 70 degrees C for R-5P. Together, it is suggested that the P. horikoshii RNase P proteins are predominantly involved in optimization of the pRNA conformation, though they are individually dispensable for RNase P activity in vitro.

  20. Spectroscopic Confirmation of MASTER OT J010603.18-744715.8 as a Classical Nova in the SMC

    NASA Astrophysics Data System (ADS)

    Williams, S. C.; Darnley, M. J.

    2016-10-01

    We obtained a spectrum of the Small Magellanic Cloud nova candidate MASTER OT J010603.18-744715.8 (ATel #9621, #9622) with the FLOYDS instrument on the 2m Faulkes Telescope South (Siding Spring Observatory, NSW, Australia) on 2016 Oct 14.70 UT. The spectral range covers 3200 Å to 1.1 & mu;m at an approximate resolution of R ~ 500. The spectrum shows strong Balmer emission (H & alpha;, H & beta;, H & gamma;, H & delta;, H & epsilon;, H8 and H9) and we measure the FWHM from H & alpha; and H & beta; to be ~ 3700 km/s.

  1. Fermi and Swift observations of correlated outburst activity from the BL Lac object OT 081 (PKS 1749+096)

    NASA Astrophysics Data System (ADS)

    Ciprini, Stefano; Gonzalez, Josefa Becerra; Pivato, Giovanna; Thompson, David J.

    2016-07-01

    We report a strong multi-wavelength outburst in the BL Lac object OT 081 (also known as PKS 1749+096, 4C +09.57) with the radio counterpart position R.A.: 267.88674 deg, Dec.: 9.65020 deg (J2000.0, Lanyi et al. 2010, AJ, 139, 1695) and with redshift z=0.322 (Stickel, Fried, Kuehr 1988, A & A, 191, 16). Gamma ray and X-ray flares reached peaks during the interval 2016 July 16 and 20 simultaneous with an optical outburst (Balonek et al, 2016, ATel #9259).

  2. Fermi-LAT detection of a GeV gamma-ray flare from the BL Lac object OT 081

    NASA Astrophysics Data System (ADS)

    Becerra Gonzalez, J.; Thompson, D.; Fermi-LAT Collaboration

    2016-07-01

    The Large Area Telescope (LAT), one of the two instruments on the Fermi Gamma-ray Space Telescope, has observed increasing gamma-ray flux from a source positionally consistent with the BL Lac object OT 081 (also known as PKS 1749+096, 4C 09.57, 0FGL J1751.5+0935, 1FGL J1751.5+0937, 2FGL J1751.5+0938, 1FHL J1751.5+0938, 3FGL J1751.5+0939), with the radio counterpart position R.A.: 267.88674 deg, Dec.: 9.65020 deg (J2000.0, Lanyi et al. 2010, AJ, 139, 1695).

  3. Three complete turns of a 3(10)-helix at atomic resolution: the crystal structure of Z-(Aib)11-OtBu.

    PubMed

    Gessmann, Renate; Brückner, Hans; Petratos, Kyriacos

    2003-01-01

    The crystal structure of the synthetic protected oligopeptide Z-(Aib)11-OtBu was determined by x-ray crystallography. The undecapeptide folds in a regular 3(10)-helix with nine consecutive 4 --> 1 hydrogen bonds. At present, this is the largest available structure of a homopeptide (including homopeptides consisting of standard amino acids) and also the longest observed regular 3(10)-helix at atomic resolution. Z-(Aib)11-OtBu crystallizes readily from hot ethanol-water mixture and is one of the crystals in which no solvent molecule is co-crystallized. In the crystal head-to-tail hydrogen bonded columns are formed in the [1 0 1] direction. Each helical column is surrounded by six others, whereby two are packed in parallel and four in antiparallel fashion. Helical columns are packed via apolar crystal contacts. The crystal structure of Z-(Aib)11-OtBu is compared with the crystal structures of Z-(Aib)10-OtBu and Z-(Aib)9-OtBu. The similarities and differences are analysed.

  4. Purification, crystallization and preliminary crystallographic analysis of the vacuole-type ATPase subunit E from Pyrococcus horikoshii OT3

    SciTech Connect

    Lokanath, Neratur K.; Ukita, Yoko; Sugahara, Mitsuaki; Kunishima, Naoki

    2005-01-01

    The E subunit of vacuole-type ATPase from P. horikoshii OT3 was overexpressed, purified and crystallized. The native crystals diffracted X-rays to 1.85 Å resolution. The vacuole-type ATPases in eukaryotic cells translocate protons across various biological membranes including the vacuolar membrane by consuming ATP molecules. The E subunit of the multisubunit complex V-ATPase from Pyrococcus horikoshii OT3, which has a molecular weight of 22.88 kDa, has been cloned, overexpressed in Escherichia coli, purified and crystallized by the microbatch method using PEG 4000 as a precipitant at 296 K. A data set to 1.85 Å resolution with 98.8% completeness and an R{sub merge} of 6.5% was collected from a single flash-cooled crystal using synchrotron radiation. The crystal belonged to the orthorhombic space group P2{sub 1}2{sub 1}2{sub 1}, with unit-cell parameters a = 52.196, b = 55.317, c = 77.481 Å, and is most likely to contain one molecule per asymmetric unit.

  5. Anti-Tumor Effects after Adoptive Transfer of IL-12 Transposon-Modified Murine Splenocytes in the OT-I-Melanoma Mouse Model.

    PubMed

    Galvan, Daniel L; O'Neil, Richard T; Foster, Aaron E; Huye, Leslie; Bear, Adham; Rooney, Cliona M; Wilson, Matthew H

    2015-01-01

    Adoptive transfer of gene modified T cells provides possible immunotherapy for patients with cancers refractory to other treatments. We have previously used the non-viral piggyBac transposon system to gene modify human T cells for potential immunotherapy. However, these previous studies utilized adoptive transfer of modified human T cells to target cancer xenografts in highly immunodeficient (NOD-SCID) mice that do not recapitulate an intact immune system. Currently, only viral vectors have shown efficacy in permanently gene-modifying mouse T cells for immunotherapy applications. Therefore, we sought to determine if piggyBac could effectively gene modify mouse T cells to target cancer cells in a mouse cancer model. We first demonstrated that we could gene modify cells to express murine interleukin-12 (p35/p40 mIL-12), a transgene with proven efficacy in melanoma immunotherapy. The OT-I melanoma mouse model provides a well-established T cell mediated immune response to ovalbumin (OVA) positive B16 melanoma cells. B16/OVA melanoma cells were implanted in wild type C57Bl6 mice. Mouse splenocytes were isolated from C57Bl6 OT-I mice and were gene modified using piggyBac to express luciferase. Adoptive transfer of luciferase-modified OT-I splenocytes demonstrated homing to B16/OVA melanoma tumors in vivo. We next gene-modified OT-I cells to express mIL-12. Adoptive transfer of mIL-12-modified mouse OT-I splenocytes delayed B16/OVA melanoma tumor growth in vivo compared to control OT-I splenocytes and improved mouse survival. Our results demonstrate that the piggyBac transposon system can be used to gene modify splenocytes and mouse T cells for evaluating adoptive immunotherapy strategies in immunocompetent mouse tumor models that may more directly mimic immunotherapy applications in humans. PMID:26473608

  6. Anti-Tumor Effects after Adoptive Transfer of IL-12 Transposon-Modified Murine Splenocytes in the OT-I-Melanoma Mouse Model

    PubMed Central

    Foster, Aaron E.; Huye, Leslie; Bear, Adham; Rooney, Cliona M.; Wilson, Matthew H.

    2015-01-01

    Adoptive transfer of gene modified T cells provides possible immunotherapy for patients with cancers refractory to other treatments. We have previously used the non-viral piggyBac transposon system to gene modify human T cells for potential immunotherapy. However, these previous studies utilized adoptive transfer of modified human T cells to target cancer xenografts in highly immunodeficient (NOD-SCID) mice that do not recapitulate an intact immune system. Currently, only viral vectors have shown efficacy in permanently gene-modifying mouse T cells for immunotherapy applications. Therefore, we sought to determine if piggyBac could effectively gene modify mouse T cells to target cancer cells in a mouse cancer model. We first demonstrated that we could gene modify cells to express murine interleukin-12 (p35/p40 mIL-12), a transgene with proven efficacy in melanoma immunotherapy. The OT-I melanoma mouse model provides a well-established T cell mediated immune response to ovalbumin (OVA) positive B16 melanoma cells. B16/OVA melanoma cells were implanted in wild type C57Bl6 mice. Mouse splenocytes were isolated from C57Bl6 OT-I mice and were gene modified using piggyBac to express luciferase. Adoptive transfer of luciferase-modified OT-I splenocytes demonstrated homing to B16/OVA melanoma tumors in vivo. We next gene-modified OT-I cells to express mIL-12. Adoptive transfer of mIL-12-modified mouse OT-I splenocytes delayed B16/OVA melanoma tumor growth in vivo compared to control OT-I splenocytes and improved mouse survival. Our results demonstrate that the piggyBac transposon system can be used to gene modify splenocytes and mouse T cells for evaluating adoptive immunotherapy strategies in immunocompetent mouse tumor models that may more directly mimic immunotherapy applications in humans. PMID:26473608

  7. Glucuronidation of OTS167 in Humans Is Catalyzed by UDP-Glucuronosyltransferases UGT1A1, UGT1A3, UGT1A8, and UGT1A10

    PubMed Central

    Ramírez, Jacqueline; Mirkov, Snezana; House, Larry K.

    2015-01-01

    OTS167 is a potent maternal embryonic leucine zipper kinase inhibitor undergoing clinical testing as antineoplastic agent. We aimed to identify the UDP-glucuronosyltransferases (UGTs) involved in OTS167 metabolism, study the relationship between UGT genetic polymorphisms and hepatic OTS167 glucuronidation, and investigate the inhibitory potential of OTS167 on UGTs. Formation of a single OTS167-glucuronide (OTS167-G) was observed in pooled human liver (HLM) (Km = 3.4 ± 0.2 µM), intestinal microsomes (HIM) (Km = 1.7 ± 0.1 µM), and UGTs. UGT1A1 (64 µl/min/mg) and UGT1A8 (72 µl/min/mg) exhibited the highest intrinsic clearances (CLint) for OTS167, followed by UGT1A3 (51 µl/min/mg) and UGT1A10 (47 µl/min/mg); UGT1A9 was a minor contributor. OTS167 glucuronidation in HLM was highly correlated with thyroxine glucuronidation (r = 0.91, P < 0.0001), SN-38 glucuronidation (r = 0.79, P < 0.0001), and UGT1A1 mRNA (r = 0.72, P < 0.0001). Nilotinib (UGT1A1 inhibitor) and emodin (UGT1A8 and UGT1A10 inhibitor) exhibited the highest inhibitory effects on OTS167-G formation in HLM (68%) and HIM (47%). We hypothesize that OTS167-G is an N-glucuronide according to mass spectrometry. A significant association was found between rs6706232 and reduced OTS167-G formation (P = 0.03). No or weak UGT inhibition (range: 0–21%) was observed using clinically relevant OTS167 concentrations (0.4–2 µM). We conclude that UGT1A1 and UGT1A3 are the main UGTs responsible for hepatic formation of OTS167-G. Intestinal UGT1A1, UGT1A8, and UGT1A10 may contribute to first-pass OTS167 metabolism after oral administration. PMID:25870101

  8. AVESTAR Center for operational excellence of clean energy plants and DYNSIM OTS / EyeSim ITS integration

    SciTech Connect

    Provost, G

    2012-01-01

    This Power-Point presentation with notes starts with a brief overview of US energy challenging, particularly as regards power generation capacity and clean energy plant operations. It then goes on to present Advanced Virtual Energy Simulation Training And Research (AVESTAR{trademark}) beginning with a statement of its missions and goals, then moves to the subject of Integrated Gasification Combined Cycle (IGCC) with CO{sub 2} Capture, first providing a brief overview of the process, then moving on to Dynamic Simulator/Operator Training System (OTS) and 3D Virtual Immersive Training System (ITS). The presentation continues to describe AVESTAR center facilities, locations, and training systems and to look at future directions for virtual energy simulation.

  9. DUSTY EXPLOSIONS FROM DUSTY PROGENITORS: THE PHYSICS OF SN 2008S AND THE 2008 NGC 300-OT

    SciTech Connect

    Kochanek, C. S.

    2011-11-01

    SN 2008S and the 2008 NGC 300-OT were explosive transients of stars self-obscured by very dense, dusty stellar winds. An explosive transient with an unobserved shock breakout luminosity of order 10{sup 10} L{sub sun} is required to render the transients little obscured and visible in the optical at their peaks. Such a large breakout luminosity then implies that the progenitor stars were cool, red supergiants, most probably {approx}9 M{sub sun} extreme asymptotic giant branch stars. As the shocks generated by the explosions propagate outward through the dense wind, they produce a shock luminosity in soft X-rays that powers the long-lived luminosity of the transients. Unlike typical cases of transients exploding into a surrounding circumstellar medium, the progenitor winds in these systems are optically thick to soft X-rays, easily absorb radio emission, and rapidly reform dust destroyed by the peak luminosity of the transients. As a result, X-rays are absorbed by the gas and the energy is ultimately radiated by the reformed dust. Three years post-peak, both systems are still significantly more luminous than their progenitor stars, but they are again fully shrouded by the reformed dust and only visible in the mid-IR. The high luminosity and heavy obscuration may make it difficult to determine the survival of the progenitor stars for {approx}10 years. However, our model indicates that SN 2008S, but not the NGC 300-OT, should now be a detectable X-ray source. SN 2008S has a higher estimated shock velocity and a lower density wind, so the X-rays begin to escape at a much earlier phase.

  10. Spectrum of M31 nova candidate M31N 2016-04a (MASTER OT J004528.12+414117.6)

    NASA Astrophysics Data System (ADS)

    Williams, S. C.; Darnley, M. J.

    2016-06-01

    We obtained a spectrum of nova candidate M31N 2016-04a (MASTER OT J004528.12+414117.6; ATel #8950) with the SPRAT spectrograph (Piascik et al. 2014) on the 2m Liverpool Telescope (Steele et al. 2004) on 2016 June 3.19 UT, 54 days after discovery.

  11. /sup 45/Ca efflux for myometrial cells: comparison of the effects of prostaglandin F/sub 2/. cap alpha. (PGF/sub 2/), oxytocin (OT) and arachidonate (A)

    SciTech Connect

    Katona, G.; Molnar, M.; Toth, M.; Hertelendy, F.

    1986-03-01

    The aim of this study was to measure PGF/sub 2..cap alpha../-induced Ca/sup 2 +/ release from uterine cells and to compare this to the actions of OT and A. Smooth muscle cells isolated from the uterus (shell gland) of laying hens were cultured for 7 days in M199 plus 10% fetal calf serum. The cells were treated with digitonin (20..mu..M) and preloaded with /sup 45/Ca for 40 min. Addition of PGF/sub 2..cap alpha../ caused a biphasic /sup 45/Ca-efflux. There was a small but significant /sup 45/Ca-release within 30 sec (rapid phase) followed by a larger one within 7 min (slow phase). In comparison, both OT and A stimulated /sup 45/Ca efflux during a single, slow phase. The maximal effect of A was observed at < 7 min, whereas that of OT was slower, peaking after 7 min. Mepacrin, an inhibitor of A release, attenuated the action of OT without having any effect on A promoted /sup 45/Ca-efflux. Indomethacin, an inhibitor of PG synthase, failed to suppress the Ca-releasing effect of A suggesting the A itself or a lipoxygenase product may have been responsible for the observed effects. Moreover, these results provide suggestive evidence that A release is an important step in the action of various uterotonic agents converging on the mobilization of intracellular Ca.

  12. MAGIC detects very high energy gamma-ray emission from the blazar OT 081 (PKS 1749+096, 4C +09.57)

    NASA Astrophysics Data System (ADS)

    Mirzoyan, Razmik

    2016-07-01

    The MAGIC collaboration reports on the detection of very high energy (VHE; E > 150 GeV) gamma-ray emission from OT 081 (RA=17 51 32.82, dec=+09 39 00.73, J2000.0; also known as PKS 1749+096 and 4C +09.57).

  13. THE DIVERSITY OF MASSIVE STAR OUTBURSTS. I. OBSERVATIONS OF SN2009ip, UGC 2773 OT2009-1, AND THEIR PROGENITORS

    SciTech Connect

    Foley, Ryan J.; Berger, Edo; Challis, Peter J.; Soderberg, Alicia M.; Fox, Ori; Levesque, Emily M.; Ivans, Inese I.; Rhoads, James E.

    2011-05-01

    Despite both being outbursts of luminous blue variables (LBVs), SN 2009ip and UGC 2773 OT2009-1 have very different progenitors, spectra, circumstellar environments, and possibly physical mechanisms that generated the outbursts. From pre-eruption Hubble Space Telescope images, we determine that SN 2009ip and UGC 2773 OT2009-1 have initial masses of {approx}> 60 and {approx}> 25 M{sub sun}, respectively. Optical spectroscopy shows that at peak, SN 2009ip had a 10,000 K photosphere and its spectrum was dominated by narrow H Balmer emission, similar to classical LBV giant outbursts, also known as 'supernova impostors'. The spectra of UGC 2773 OT2009-1, which also have narrow H{alpha} emission, are dominated by a forest of absorption lines, similar to an F-type supergiant. Blueshifted absorption lines corresponding to ejecta at a velocity of 2000-7000 km s{sup -1} are present in later spectra of SN 2009ip-an unprecedented observation for LBV outbursts, indicating that the event was the result of a supersonic explosion rather than a subsonic outburst. The velocity of the absorption lines increases between two epochs, suggesting that there were two explosions in rapid succession. A rapid fading and rebrightening event concurrent with the onset of the high-velocity absorption lines is consistent with the double-explosion model. A near-infrared excess is present in the spectra and photometry of UGC 2773 OT2009-1 that is consistent with {approx}2100 K dust emission. We compare the properties of these two events and place them in the context of other known massive star outbursts such as {eta} Car, NGC 300 OT2008-1, and SN 2008S. This qualitative analysis suggests that massive star outbursts have many physical differences that can manifest as the different observables seen in these two interesting objects.

  14. Characterization of the PH1704 Protease from Pyrococcus horikoshii OT3 and the Critical Functions of Tyr120

    PubMed Central

    Yu, Lei; Han, Weiwei; Feng, Yan

    2014-01-01

    The PH1704 protease from hyperthermophilic archaean Pyrococcus horikoshii OT3 is a member of DJ-1/ThiJ/PfpI superfamily with diverse functional subclasses. The recombinant PH1704 was efficiently purified and was systematically characterized by a combination of substrate specificity analysis, steady-state kinetics study and molecular docking research. The homogeneous protease was obtained as a presumed dodecamer with molecular weight of ∼240 kDa. Iodoacetamide strongly inhibited the peptidase activity, confirming that Cys100 is a nucleophilic residue. The recombinant protein was identified as both an aminopeptidase and an endopeptidase. Experimental data showed that L-R-amc was the best substrate of PH1704. Structural interaction fingerprint analysis (SIFt) indicated the binding pose of PH1704 and showed that Tyr120 is important in substrate binding. Kinetic parameters Kcat and Kcat/Km of the Y120P mutant with L-R-amc was about 7 and 7.8 times higher than that of the wild type (WT). For the endopeptidase Y120P with AAFR-amc, Kcat and Kcat/Km is 10- and 21- fold higher than that of WT. Experimental data indicate the important functions of Tyr120: involvement in enzyme activity to form a hydrogen bond with Cys100 and as an entrance gate of the substrate with Lys43. The results of this study can be used to investigate the DJ-1/ThiJ/PfpI superfamily. PMID:25192005

  15. Dynamics of acrylodan-labeled bovine and human serum albumin sequestered within aerosol-OT reverse micelles.

    PubMed

    Lundgren, J S; Heitz, M P; Bright, F V

    1995-10-15

    We investigate the effects of hydration on acrylodan-labeled bovine and human serum albumin (BSA-Ac and HSA-Ac) in aerosol-OT (AOT) reverse micelles solubilized in n-heptane. Time-resolved fluorescence intensity decay experiments reveal a dipolar relaxation process surrounding the acrylodan cybotactic region. This process is best described by a two-term rate law wherein the average relaxation increases with increased hydration. However, the actual rate constants describing the relaxation process either remain unchanged or actually decrease with increased hydration. The results illustrate that the fractional contribution associated with the individual relaxation pathways causes the observed changes in relaxation dynamics. The recovered rotational reorientation dynamics of the acrylodan residue are also affected by the extent of protein hydration. As hydration is increased, the semiangle through which the acrylodan residue precesses increases by 10 degrees for both protein systems. Interestingly, the recovered semiangles for the native proteins equal those recovered at lower hydration when the proteins are sequestered within the AOT reverse micelle. These results demonstrate the importance of hydration on protein behavior in environments where water is limited (e.g., biosensor interfaces and sol--gel-derived biocomposites).

  16. Genome sequence of Ostreococcus tauri virus OtV-2 throws light on the role of picoeukaryote niche separation in the ocean.

    PubMed

    Weynberg, Karen D; Allen, Michael J; Gilg, Ilana C; Scanlan, David J; Wilson, William H

    2011-05-01

    Ostreococcus tauri, a unicellular marine green alga, is the smallest known free-living eukaryote and is ubiquitous in the surface oceans. The ecological success of this organism has been attributed to distinct low- and high-light-adapted ecotypes existing in different niches at a range of depths in the ocean. Viruses have already been characterized that infect the high-light-adapted strains. Ostreococcus tauri virus (OtV) isolate OtV-2 is a large double-stranded DNA algal virus that infects a low-light-adapted strain of O. tauri and was assigned to the algal virus family Phycodnaviridae, genus Prasinovirus. Our working hypothesis for this study was that different viruses infecting high- versus low-light-adapted O. tauri strains would provide clues to propagation strategies that would give them selective advantages within their particular light niche. Sequence analysis of the 184,409-bp linear OtV-2 genome revealed a range of core functional genes exclusive to this low-light genotype and included a variety of unexpected genes, such as those encoding an RNA polymerase sigma factor, at least four DNA methyltransferases, a cytochrome b(5), and a high-affinity phosphate transporter. It is clear that OtV-2 has acquired a range of potentially functional genes from its host, other eukaryotes, and even bacteria over evolutionary time. Such piecemeal accretion of genes is a trademark of large double-stranded DNA viruses that has allowed them to adapt their propagation strategies to keep up with host niche separation in the sunlit layers of the oceanic environment. PMID:21289127

  17. The Chromate-Inducible chrBACF Operon from the Transposable Element TnOtChr Confers Resistance to Chromium(VI) and Superoxide▿

    PubMed Central

    Branco, Rita; Chung, Ana Paula; Johnston, Tatiana; Gurel, Volkan; Morais, Paula; Zhitkovich, Anatoly

    2008-01-01

    Large-scale industrial use of chromium(VI) has resulted in widespread contamination with carcinogenic chromium(VI). The abilities of microorganisms to survive in these environments and to detoxify chromate require the presence of specific resistance systems. Here we report identification of the transposon-located (TnOtChr) chromate resistance genes from the highly tolerant strain Ochrobactrum tritici 5bvl1 surviving chromate concentrations of >50 mM. The 7,189-bp-long TnOtChr of the mixed Tn21/Tn3 transposon subfamily contains a group of chrB, chrA, chrC, and chrF genes situated between divergently transcribed resolvase and transposase genes. The chrB and chrA genes, but not chrF or chrC, were essential for establishment of high resistance in chromium-sensitive O. tritici. The chr promoter was strongly induced by chromate or dichromate, but it was completely unresponsive to Cr(III), oxidants, sulfate, or other oxyanions. Plasmid reporter experiments identified ChrB as a chromate-sensing regulator of chr expression. Induction of the chr operon suppressed accumulation of cellular Cr through the activity of a chromate efflux pump encoded by chrA. Expression of chrB, chrC, or chrF in an Escherichia coli sodA sodB double mutant restored its aerobic growth in minimal medium and conferred resistance to superoxide-generating agents menadione and paraquat. Nitroblue tetrazolium staining on native gels showed that ChrC protein had superoxide dismutase activity. TnOtChr appears to represent a mobile genetic system for the distribution of the chromate-regulated resistance operon. The presence of three genes protecting against superoxide toxicity should provide an additional survival advantage to TnOtChr-containing cells in the environments with multiple redox-active contaminants. PMID:18776016

  18. Life-Cycle and Genome of OtV5, a Large DNA Virus of the Pelagic Marine Unicellular Green Alga Ostreococcus tauri

    PubMed Central

    Derelle, Evelyne; Ferraz, Conchita; Escande, Marie-Line; Eychenié, Sophie; Cooke, Richard; Piganeau, Gwenaël; Desdevises, Yves; Bellec, Laure; Moreau, Hervé; Grimsley, Nigel

    2008-01-01

    Large DNA viruses are ubiquitous, infecting diverse organisms ranging from algae to man, and have probably evolved from an ancient common ancestor. In aquatic environments, such algal viruses control blooms and shape the evolution of biodiversity in phytoplankton, but little is known about their biological functions. We show that Ostreococcus tauri, the smallest known marine photosynthetic eukaryote, whose genome is completely characterized, is a host for large DNA viruses, and present an analysis of the life-cycle and 186,234 bp long linear genome of OtV5. OtV5 is a lytic phycodnavirus which unexpectedly does not degrade its host chromosomes before the host cell bursts. Analysis of its complete genome sequence confirmed that it lacks expected site-specific endonucleases, and revealed the presence of 16 genes whose predicted functions are novel to this group of viruses. OtV5 carries at least one predicted gene whose protein closely resembles its host counterpart and several other host-like sequences, suggesting that horizontal gene transfers between host and viral genomes may occur frequently on an evolutionary scale. Fifty seven percent of the 268 predicted proteins present no similarities with any known protein in Genbank, underlining the wealth of undiscovered biological diversity present in oceanic viruses, which are estimated to harbour 200Mt of carbon. PMID:18509524

  19. Perovskite LaFeO3 nanoparticles synthesized by the reverse microemulsion nanoreactors in the presence of aerosol-OT: Morphology, crystal structure, and their optical properties

    NASA Astrophysics Data System (ADS)

    Abazari, Reza; Sanati, Soheila

    2013-12-01

    Orthorhombic structure of lanthanum ferrite nanoparticles (LaFeO3 NPs) with perovskite type phase has been synthesized with water-in-oil (W/O) microemulsion consisted of water/dioctyl sulfosuccinate sodium (aerosol-OT)/isooctane at room temperature. It has been shown that aerosol-OT reverse microemulsion solution is appropriate for synthesizing perovskite LaFeO3 NPs in the absence of any co-surfactants. Field emission scanning electron microscope (FE-SEM), transmission electron microscopy (TEM), energy dispersive analysis of X-ray (EDAX), X-ray diffraction (XRD), ultraviolet-visible absorption spectroscopy (UV-Vis), and Fourier transform infrared spectroscopy (FT-IR) have been adopted for characterization of surface morphology, size, phase composition, structure, and optical properties of the considered NPs. Furthermore, the optical properties of LaFeO3 NPs have been further analyzed via photoluminescence (PL) spectroscopy. As shown by the physicochemical characterizations, our prepared NPs via aerosol-OT reverse microemulsion solution are spherical and nearly uniform (with a size of about 24.65 nm). Besides, they include an orthorhombic phase while no impurities are observed. Single phase lanthanum ferrite NPs have successfully been prepared at 500 °C. Moreover, UV-Vis spectrum indicates that the LaFeO3 NPs synthesized through this technique can be considered as a type of photo-catalytic materials.

  20. Automatic parameter adjustment of difference of Gaussian (DoG) filter to improve OT-MACH filter performance for target recognition applications

    NASA Astrophysics Data System (ADS)

    Alkandri, Ahmad; Gardezi, Akber; Bangalore, Nagachetan; Birch, Philip; Young, Rupert; Chatwin, Chris

    2011-11-01

    A wavelet-modified frequency domain Optimal Trade-off Maximum Average Correlation Height (OT-MACH) filter has been trained using 3D CAD models and tested on real target images acquired from a Forward Looking Infra Red (FLIR) sensor. The OT-MACH filter can be used to detect and discriminate predefined targets from a cluttered background. The FLIR sensor extends the filter's ability by increasing the range of detection by exploiting the heat signature differences between the target and the background. A Difference of Gaussians (DoG) based wavelet filter has been use to improve the OT-MACH filter discrimination ability and distortion tolerance. Choosing the right standard deviation values of the two Gaussians comprising the filter is critical. In this paper we present a new technique for auto adjustment of the DoG filter parameters driven by the expected target size. Tests were carried on images acquired by the Apache AH-64 helicopter mounted FLIR sensor, results showing an overall improvement in the recognition of target objects present within the IR images.

  1. FT-IR spectroscopy and density functional theory calculations of 13C isotopologues of the helical peptide Z-Aib6-OtBu.

    PubMed

    Zeko, Timothy; Hannigan, Steven F; Jacisin, Timothy; Guberman-Pfeffer, Matthew J; Falcone, Eric R; Guildford, Melissa J; Szabo, Christopher; Cole, Kathryn E; Placido, Jessica; Daly, Erin; Kubasik, Matthew A

    2014-01-01

    Isotope-edited FT-IR spectroscopy is a combined synthetic and spectroscopic method used to characterize local (e.g., residue-level) vibrational environments of biomolecules. We have prepared the 3(10) helical peptide Z-Aib6-OtBu and seven (13)C-enriched analogues that vary only in the number and position(s) of (13)C═O isotopic enrichment. FT-IR spectra of these eight peptides solvated in the nonpolar aprotic solvent dichloromethane have been collected and compared to frequency, intensity, and normal mode results of DFT calculations. Single (13)C enrichment of amide functional groups tends to localize amide I vibrational eigenmodes, providing residue-specific information regarding the local environment (e.g., hydrogen bonding or solvent exposure) of the peptide bond. Double (13)C enrichment of Z-Aib6-OtBu allows for examination of interamide coupling between two labeled amide functional groups, providing experimental evidence of interamide coupling in the context of 3(10) helical structure. Although the calculated and observed interamide couplings of Z-Aib6-OtBu are a few cm(-1) and less, the eight peptides exhibit distinct infrared spectra, revealing details of interamide coupling and residue level vibrational environments.

  2. The Effect of MicroRNA-375 Overexpression, an Inhibitor of Helicobacter pylori-Induced Carcinogenesis, on lncRNA SOX2OT

    PubMed Central

    Shafiee, Mohammad; Aleyasin, Seyed Ahmad; Mowla, Seyed Javad; Vasei, Mohammad; Yazdanparast, Seyed Amir

    2016-01-01

    Background Helicobacter pylori is a major human pathogenic bacterium in gastric mucosa. Although the association between gastric cancer and H. pylori has been well-established, the molecular mechanisms underlying H. pylori-induced carcinogenesis are still under investigation. MicroRNAs (miRNAs) are small noncoding RNAs that modulate gene expression at the posttranscriptional level. Recently, studies have revealed that miRNAs are involved in immune response and host cell response to bacteria. Also, microRNA-375 (miR-375) is a key regulator of epithelial properties that are necessary for securing epithelium-immune system cross-talk. It has been recently reported that miR-375 acts as an inhibitor of H. pylori-induced gastric carcinogenesis. There are few reports on miRNA-mediated targeting long noncoding RNAs (lncRNAs). Objectives This study aimed to examine the possible effect of miR-375 as an inhibitor of H. pylori-induced carcinogenesis on the expression of lncRNA SOX2 overlapping transcript (SOX2OT) and SOX2, a master regulator of pluripotency of cancer stem cells. Materials and Methods In a model cell line, NT-2 was transfected with the constructed expression vector pEGFP-C1 contained miR-375. The RNA isolations and cDNA synthesis were performed after 48 hours of transformation. Expression of miR-375 and SOX2OT and SOX2 were quantified using real-time polymerase chain reaction and compared with control cells transfected with pEGFP-C1-Mock clone. Cell cycle modification was also compared after transfections using the flow cytometry analysis. Results Following ectopic expression of miR-375, SOX2OT and SOX2 expression analysis revealed a significant decrease in their expression level (P < 0.05) in NT-2 cells compared to the control. Cell cycle analysis following ectopic expression of miR-375 in the NT-2 cells using propidium iodine staining revealed significant extension in sub-G1 cell cycle. Conclusions This is the first report to show down-regulation of SOX2OT and

  3. OT2_gstacey_6: PACS Oxygen Spectroscopy of High Velocity [CII] Outflows in PG1206+459

    NASA Astrophysics Data System (ADS)

    Stacey, G.

    2011-09-01

    We have discovered extreme velocity [CII] 158 um line emission from the z = 1.2 hyper-luminous QSO PG 1206+459. The [CII] line emission is concentrated in twin lobes of emission roughly centered on the CO emission but displaced +/- 1500 km/s with respect to the CO line core. The mass traced in the [CII] line is quite large - about 5E9 M(solar) in each lobe or 13% of the total molecular gas mass in the host galaxy. We interpret this symmetric [CII] velocity structure as an outflow, likely driven by the AGN. The velocities of the [CII] lobes are larger than the escape velocity, so much of this material may leave the galaxy quenching both star formation and AGN activity, and transforming it into a "red-and-dead" passive galaxy. The CO line profile also shows a broad and blueshifted component that may represent an additional molecular component to the outflow, although a merger origin is also possible. In OT1 we were awarded 2.9 hours of PACS spectroscopy (not yet scheduled) to observe the [OI] 63 um, [OIII] 52 and 88 um, and [OIV] 26 um lines from the blue component of the line. Here we ask for an additional 10.2 hours of PACS time to widen our spectral scan to enclose the newly discovered (March 2011) red lobe and significantly improve our detection limit over the entire velocity range. These observations will be used to constrain the physical conditions, ionization structure, total mass, and radiative environment of the outflowing material using extinction-free probes. Our [CII] observations of PG1206 represent the first time that massive outflows have been detected in the [CII] line - a well established tracer of photodissociated molecular gas. The proposed Herschel observations will lay the groundwork for future studies of molecular outflows using [CII] and other far-IR lines in the early universe that will soon be possible with ALMA.

  4. Simultaneous Photometry and Spectroscopy of the Deeply Absorbing Polar MASTER OT J132104.04+560957.8

    NASA Astrophysics Data System (ADS)

    Hoyt, Taylor; Littlefield, Colin; Garnavich, Peter M.

    2016-01-01

    We present photometry and time-resolved spectroscopy of the suspected polar MASTER OT J132104.04+560957.8 (J1321) taken at the Large Binocular Telescope (LBT) simultaneously with the LBC camera and MODS spectrograph in binocular mode. Our V-band photometry confirmed a primary and secondary maximum first identified by Littlefield et al. In the spectra we see unusually deep Balmer and helium absorption features for about 10-15% of a 91 minute orbital period. The absorption lines first appear to the red of the emission peaks then shift bluewards, ultimately dominating the line profile at zero radial velocity. Towards the end of the absorption phase, weak absorption reappears to the blue of the emission lines. When the system is bright the spectra show a broadly-peaked continuum with a maximum around 500nm. We do not see absorption during the secondary bright red phase. Around the minima the continuum is blue and resembles a more typical polar spectrum with strong Balmer, neutral helium and ionized helium emission lines. The Doppler tomograms of J1321 are fuzzy and blobby and resemble those of the asynchronous polar BY Cam. The highly variable width of the secondary maximum remains a mystery along with the lack of a discernible irradiated secondary in the tomograms. We use our observations to constrain the properties of the white dwarf's magnetic field as well as the accretion flow structure. We also discuss evidence of J1321 being in a prolonged low-mass-transfer state in all observations prior to 2007.

  5. ADP-dependent 6-Phosphofructokinase from Pyrococcus horikoshii OT3: STRUCTURE DETERMINATION AND BIOCHEMICAL CHARACTERIZATION OF PH1645

    SciTech Connect

    Currie, Mark A.; Merino, Felipe; Skarina, Tatiana; Wong, Andrew H.Y.; Singer, Alexander; Brown, Greg; Savchenko, Alexei; Caniuguir, Andrés; Guixé, Victoria; Yakunin, Alexander F.; Jia, Zongchao

    2009-12-01

    Some hyperthermophilic archaea use a modified glycolytic pathway that employs an ADP-dependent glucokinase (ADP-GK) and an ADP-dependent phosphofructokinase (ADP-PFK) or, in the case of Methanococcus jannaschii, a bifunctional ADP-dependent glucophosphofructokinase (ADP-GK/PFK). The crystal structures of three ADP-GKs have been determined. However, there is no structural information available for ADP-PFKs or the ADP-GK/PFK. Here, we present the first crystal structure of an ADP-PFK from Pyrococcus horikoshii OT3 (PhPFK) in both apo- and AMP-bound forms determined to 2.0-{angstrom} and 1.9-{angstrom} resolution, respectively, along with biochemical characterization of the enzyme. The overall structure of PhPFK maintains a similar large and small {alpha}/{beta} domain structure seen in the ADP-GK structures. A large conformational change accompanies binding of phosphoryl donor, acceptor, or both, in all members of the ribokinase superfamily characterized thus far, which is believed to be critical to enzyme function. Surprisingly, no such conformational change was observed in the AMP-bound PhPFK structure compared with the apo structure. Through comprehensive site-directed mutagenesis of the substrate binding pocket we identified residues that were critical for both substrate recognition and the phosphotransfer reaction. The catalytic residues and many of the substrate binding residues are conserved between PhPFK and ADP-GKs; however, four key residues differ in the sugar-binding pocket, which we have shown determine the sugar-binding specificity. Using these results we were able to engineer a mutant PhPFK that mimics the ADP-GK/PFK and is able to phosphorylate both fructose 6-phosphate and glucose.

  6. Unique patterns of CD8+ T-cell-mediated organ damage in the Act-mOVA/OT-I model of acute graft-versus-host disease.

    PubMed

    Érsek, Barbara; Lupsa, Nikolett; Pócza, Péter; Tóth, Anett; Horváth, Andor; Molnár, Viktor; Bagita, Bence; Bencsik, András; Hegyesi, Hargita; Matolcsy, András; Buzás, Edit I; Pós, Zoltán

    2016-10-01

    T-cell receptor (TCR)-transgenic models of acute graft-versus-host disease (aGvHD) offer a straightforward and highly controlled approach to study the mechanisms and consequences of T-cell activation following allogeneic hematopoietic stem cell transplantation (aHSCT). Here, we report that aHSCT involving OT-I mice as donors, carrying an ovalbumin-specific CD8+ TCR, and Act-mOVA mice as recipients, expressing membrane-bound ovalbumin driven by the β-actin promoter, induces lethal aGvHD in a CD8+ T-cell-dependent, highly reproducible manner, within 4-7 days. Tracking of UBC-GFP/OT-I graft CD8+ T cells disclosed heavy infiltration of the gastrointestinal tract, liver, and lungs at the onset of the disease, and histology confirmed hallmark features of gastrointestinal aGVHD, hepatic aGvHD, and aGvHD-associated lymphocytic bronchitis in infiltrated organs. However, T-cell infiltration was virtually absent in the skin, a key target organ of human aGvHD, and histology confirmed the absence of cutaneous aGVHD, as well. We show that the model allows studying CD8+ T-cell responses in situ, as selective recovery of graft CD45.1/OT-I CD8+ T cells from target organs is simple and feasible by automated tissue dissociation and subsequent cell sorting. Assessment of interferon-gamma production by flow cytometry, granzyme-B release by ELISA, TREC assay, and whole-genome gene expression profiling confirmed that isolated graft CD8+ T cells remained intact, underwent clonal expansion, and exerted effector functions in all affected tissues. Taken together, these data demonstrate that the OT-I/Act-mOVA model is suitable to study the CD8+ T-cell-mediated effector mechanisms in a disease closely resembling fatal human gastrointestinal and hepatic aGVHD that may develop after aHSCT using HLA-matched unrelated donors.

  7. The crystal structure of Z-Aib-Gly-Aib-Leu-Aib-OtBu, the synthetic, protected N-terminal pentapeptide of trichotoxin.

    PubMed

    Gessmann, R; Brueckner, H; Kokkinidis, M

    1991-01-01

    Z-Aib-Gly-Aib-Leu-Aib-OtBu, the alpha-aminoisobutyric acid (Aib)-containing N-terminal pentapeptide of the antibiotic trichotoxin, has been studied by x-ray crystallography. The molecule forms a right-handed helix with a reversal of the sense of the helix at the C-terminus. Torsion angles and hydrogen bonding pattern are consistent with a mixed 3(10)-/alpha-helical conformation. In the crystal, continuous columns are formed by head-to-tail arrangement of hydrogen-bonded molecules along the helix axis. The helical columns associate via hydrogen bonds forming closely packed parallel pairs.

  8. Visible-light driven water splitting over BiFeO3 photoanodes grown via the LPCVD reaction of [Bi(OtBu)3] and [Fe(OtBu)3]2 and enhanced with a surface nickel oxygen evolution catalyst

    NASA Astrophysics Data System (ADS)

    Moniz, Savio J. A.; Blackman, Christopher S.; Southern, Paul; Weaver, Paul M.; Tang, Junwang; Carmalt, Claire J.

    2015-10-01

    Phase-pure BiFeO3 films were grown directly via dual-source low-pressure CVD (LPCVD) from the ligand-matched precursors [Bi(OtBu)3] and [Fe(OtBu)3]2, without the requirement for oxidising gas or post deposition annealing. Photocatalytic testing for water oxidation revealed extremely high activity for PEC water splitting and photocatalytic water oxidation under visible light irradiation (λ > 420 nm) with a benchmark IPCE for BiFeO3 of 23% at 400 nm. The high activity is ascribed to the ultrafine morphology achieved via the LPCVD process. The performance was enhanced by over four times when the BiFeO3 photoanode is coupled to a Ni-B surface OEC.Phase-pure BiFeO3 films were grown directly via dual-source low-pressure CVD (LPCVD) from the ligand-matched precursors [Bi(OtBu)3] and [Fe(OtBu)3]2, without the requirement for oxidising gas or post deposition annealing. Photocatalytic testing for water oxidation revealed extremely high activity for PEC water splitting and photocatalytic water oxidation under visible light irradiation (λ > 420 nm) with a benchmark IPCE for BiFeO3 of 23% at 400 nm. The high activity is ascribed to the ultrafine morphology achieved via the LPCVD process. The performance was enhanced by over four times when the BiFeO3 photoanode is coupled to a Ni-B surface OEC. Electronic supplementary information (ESI) available. See DOI: 10.1039/c5nr04804d

  9. Growth of a radiation-transformed clone of C3H 1OT1/2 cells into melanin-producing colonies

    SciTech Connect

    Szekely, J.G.; Raaphorst, G.P.; Lobreau, A.U.; Azzam, E.I.; Vadasz, J.A.

    1985-01-01

    When R25, a radiation-transformed clone of C3H 1OT1/2 cells, was plated in soft agarose, a fraction of the colonies became pigmented. The morphologies of the white and dark colonies and their cells were compared by optical, transmission and scanning electron microscopy. The transformed R25 cells had apparently differentiated into melanin-producing cells in the soft agarose, with the white colonies containing actively growing cells having only a few melanosomes, and the dark colonies being made up of stationary-phase cells filled with electronopaque melanosomes. Exposure of the R25 cells to 4.0 Gy of X-rays decreased the percentage of dark colonies, while exposure to 1% DMSO had no effect.

  10. Effect of hydration degree of aerosol OT reversed micelles and surfactant concentration in heptane on spectral and catalytic properties of catalase.

    PubMed

    Eryomin, A N; Metelitza, D I

    1999-09-01

    The influence of micelle hydration degree (w0) and AOT concentration on fluorescence, circular dichroism (CD), catalytic activity, and stability of catalase in Aerosol OT (AOT) reversed micelles in heptane was investigated. The quantitative parameters--differential fluorescence of catalase (DeltaI), protein molar ellipticity ([theta]lambda), initial rate of catalytic reaction, catalase efficiency (kcat/Km), and rate constant of enzyme inactivation (kin, sec-1)--decreased with increasing AOT concentration in micellar systems, reflecting the interaction of solubilized catalase with the AOT micellar aggregates in heptane. The dependences of all these parameters on increasing hydration degree of micelles (w0) were characterized by the appearance of maxima at w0 of 8, 15-18, and 26-30. These maxima are suggested to reflect three different states of catalase in the micellar system, distinguished by their conformations and catalytic activity, which is determined by the micellar microenvironment of the enzyme. PMID:10521722

  11. Discovery of an unusual bright eclipsing binary with the longest known period: TYC 2505-672-1/MASTER OT J095310.04+335352.8

    NASA Astrophysics Data System (ADS)

    Lipunov, V.; Gorbovskoy, E.; Afanasiev, V.; Tatarnikova, A.; Denisenko, D.; Makarov, D.; Tiurina, N.; Krushinsky, V.; Vinokurov, A.; Balanutsa, P.; Kuznetsov, A.; Gress, O.; Sergienko, Yu.; Yurkov, V.; Gabovich, A.; Tlatov, A.; Senik, V.; Vladimirov, V.; Popova, E.

    2016-04-01

    We report on the MASTER Global Robotic Net discovery of an eclipsing binary, MASTER OT J095310.04+335352.8, previously known as unremarkable star TYC 2505-672-1, which displays extreme orbital parameters. The orbital period P = 69.1 yr is more than 2.5 times longer than that of ɛ-Aurigae, which is the previous record holder. The light curve is characterized by an extremely deep total eclipse with a depth of more than 4.5 mag, which is symmetrically shaped and has a total duration of 3.5 yr. The eclipse is essentially gray. The spectra acquired with the Russian 6 m BTA telescope both at minimum and maximum light mainly correspond to an M0-1III-type red giant, but the spectra taken at the bottom of eclipse show small traces of a sufficiently hot source. The observed properties of this system can be better explained as the red giant eclipsed by a large cloud (the disk) of small particles surrounding the invisible secondary companion.

  12. EPA/ORD/RTP (Environmental Protection Agency, Office of Research and Development, Research Triangle Park) asbestos program support for the OTS (Office of Toxic Substances) asbestos-in-schools program. Final report

    SciTech Connect

    Beard, M.E.

    1986-03-01

    An overview of the EPA/ORD/RTP Asbestos program support for the OTS Asbestos-in-Schools Program is given. The program includes (1) a bulk sample asbestos analysis audit program, (2) an intercomparison of three measurement methods for airborne asbestos, (3) the development of audit materials for airborne asbestos measurement methods, and (4) the development of guidance for post-abatement asbestos air monitoring.

  13. Ries Bunte Breccia revisited: Indications for the presence of water in Itzing and Otting drill cores and implications for the emplacement process

    NASA Astrophysics Data System (ADS)

    Pietrek, Alexa; Kenkmann, Thomas

    2016-07-01

    We reassessed two drill cores of the Bunte Breccia deposits of the Ries crater, Germany. The objectives of our study were the documentation of evidence for water in the Bunte Breccia, the evaluation of how that water influenced the emplacement processes, and from which preimpact water reservoir it was derived. The Bunte Breccia in both cores can be structured into a basal layer composed mainly of local substrate material, overlain by texturally and compositionally diverse, crater-derived breccia units. The basal layer is composed of the youngest sediments (Tertiary clays and Upper Jurassic limestone) and has a razor-sharp boundary to the upper breccia units, which are composed of older rocks of Upper Jurassic to Upper Triassic age. Sparse material exchange occurred between the basal layer and the rest of the Bunte Breccia. Fluids predominantly came from the Tertiary and the Upper Triassic sandstone formation. In the basal layer, Tertiary clays were subjected to intense, ductile deformation, indicating saturation with water. This suggests that water was mixed into the matrix, creating a fluidized basal layer with a strong shear localization. In the upper units, Upper Triassic sandstones are intensely deformed by granular flow. The texture requires that the rocks were disaggregated into granular sand. Vaporization of pore water probably aided fragmentation of these rocks. In the Otting core, hot suevite (T > 600 °C) covered the Bunte Breccia shortly after its emplacement. Vertically oriented gas escape pipes in suevite partly emanate directly at the contact to the Bunte Breccia. They indicate that the Bunte Breccia contained a substantial amount of water in the upper part that was vaporized and escaped through these vents.

  14. Identification of a novel amino acid racemase from a hyperthermophilic archaeon Pyrococcus horikoshii OT-3 induced by D-amino acids.

    PubMed

    Kawakami, Ryushi; Ohmori, Taketo; Sakuraba, Haruhiko; Ohshima, Toshihisa

    2015-08-01

    To date, there have been few reports analyzing the amino acid requirement for growth of hyperthermophilic archaea. We here found that the hyperthermophilic archaeon Pyrococcus horikoshii OT-3 requires Thr, Leu, Val, Phe, Tyr, Trp, His and Arg in the medium for growth, and shows slow growth in medium lacking Met or Ile. This largely corresponds to the presence, or absence, of genes related to amino acid biosynthesis in its genome, though there are exceptions. The amino acid requirements were dramatically lost by addition of D-isomers of Met, Leu, Val, allo-Ile, Phe, Tyr, Trp and Arg. Tracer analysis using (14)C-labeled D-Trp showed that D-Trp in the medium was used as a protein component in the cells, suggesting the presence of D-amino acid metabolic enzymes. Pyridoxal 5'-phosphate (PLP)-dependent racemase activity toward Met, Leu and Phe was detected in crude extract of P. horikoshii and was enhanced in cells grown in the medium supplemented with D-amino acids, especially D-allo-Ile. The gene encoding the racemase was narrowed down to one open reading frame on the basis of enzyme purification from P. horikoshii cells, and the recombinant enzyme exhibited PLP-dependent racemase activity toward several amino acids, including Met, Leu and Phe, but not Pro, Asp or Glu. This is the first report showing the presence in a hyperthermophilic archaeon of a PLP-dependent amino acid racemase with broad substrate specificity that is likely responsible for utilization of D-amino acids for growth.

  15. OT2_sserje01_2: THE HERSCHEL-AKARI NEP DEEP SURVEY: the cosmological history of stellar mass assembly and black hole accretion

    NASA Astrophysics Data System (ADS)

    Serjeant, S.

    2011-09-01

    We propose a far-IR and submm mapping survey of the premier AKARI deep field in the North Ecliptic Pole, in PACS/SPIRE parallel mode. This is the only major deep infrared field not yet covered by Herschel guaranteed or open time key projects. The outstanding and unparalleled continuous mid-IR photometric coverage from AKARI, far better than equivalent Spitzer surveys, enables a wide range of galaxy evolution diagnostics unachievable in any other survey field (including Herschel HerMES/PEP fields), by spanning the wavelengths of redshifted PAH and silicate features and the peak energy output of AGN dust tori. The investment by AKARI in the NEP represents ~10 percent of the entire pointed observations available throughout the lifetime of AKARI. Our proposal remedies the remarkable omission from Herschel's legacy surveys of the premier extragalactic deep field from another IR space telescope. We will simultaneously identify and find photometric redshifts for the Herschel point source population, make stacking analysis detections of the galaxies which dominate the submm extragalactic background light as a function of redshift, determine the bolometric power outputs of the galaxies that dominate the submm background, compare the UV/optical/mid-IR continuum/PAH/far-IR/submm/radio star formation rate estimator in the most comprehensive IR survey data set to date, and track the coupled stellar mass assembly and black hole accretion throughout most of the history of the Universe. In OT1 the HOTAC concluded "The science output from the proposed survey will be outstanding [...] The panel was convinced that these observations should be done" but it since became clear that priority 2 time is very unlikely to be executed, so we request reclassification to priority 1.

  16. Aerodynamic results of an abort separation effects test (IA8) conducted in the NASA/ARC 14-foot transonic wind tunnel on a model (6-OTS) of the Rockwell International launch configuration integrated vehicle

    NASA Technical Reports Server (NTRS)

    Campbell, J. H.

    1974-01-01

    Experimental aerodynamic investigations were conducted on a 6-OTS 0.015-scale model. The Ames dual sting support separation rig was used to obtain grid-type data for tank-booster abort from orbiter (SSV). Freestream data were obtained for the orbiter to provide a baseline for evaluation of proximity effects. Data were obtained at Mach numbers from 0.32 to 1.1, and Reynolds number per foot varying from 2.1 million to 3.9 million. Data are not presented. Because of balance failure, a very substantial portion of the test was run with a dummy balance in the tank boosters configuration.

  17. CD80 and CD86 Costimulatory Molecules Differentially Regulate OT-II CD4+ T Lymphocyte Proliferation and Cytokine Response in Cocultures with Antigen-Presenting Cells Derived from Pregnant and Pseudopregnant Mice

    PubMed Central

    Maj, Tomasz; Slawek, Anna

    2014-01-01

    Immune phenomena during the preimplantation period of pregnancy are poorly understood. The aim of our study was to assess the capacity for antigen presentation of splenic antigen-presenting cells (APCs) derived from pregnant and pseudopregnant mice in in vitro conditions. Therefore, sorted CD11c+ dendritic cells and macrophages F4/80+ and CD11b+ presenting ovalbumin (OVA) were cocultured with CD4+ T cells derived from OT-II mice's (C57BL6/J-Tg(TcraTcrb)1100Mjb/J) spleen. After 132 hours of cell culture, proliferation of lymphocytes (ELISA-BrdU), activation of these cells (flow cytometry), cytokine profile (ELISA), and influence of costimulatory molecules blocking on these parameters were measured. We did not detect any differences in regulation of Th1/Th2 cytokine balance. CD86 seems to be the main costimulatory molecule involved in the proliferation response but CD80 is the main costimulatory molecule influencing cytokine secretion in pregnant mice. In conclusion, this study showed that CD80 and CD86 costimulatory molecules regulate OT-II CD4+ T lymphocyte proliferation and cytokine response in cocultures with antigen-presenting cells derived from pregnant and pseudopregnant mice. The implications of these changes still remain unclear. PMID:24771983

  18. Investigations of the 0.020-scale 88-OTS Integrated Space Shuttle Vehicle Jet-Plume Model in the NASA/Ames Research Center 11 by11-Foot Unitary Plan Wind Tunnel (IA80). Volume 1

    NASA Technical Reports Server (NTRS)

    Nichols, M. E.

    1976-01-01

    The results are documented of jet plume effects wind tunnel test of the 0.020-scale 88-OTS launch configuration space shuttle vehicle model in the 11 x 11 foot leg of the NASA/Ames Research Center Unitary Plan Wind Tunnel. This test involved cold gas main propulsion system (MPS) and solid rocket motor (SRB) plume simulations at Mach numbers from 0.6 to 1.4. Integrated vehicle surface pressure distributions, elevon and rudder hinge moments, and wing and vertical tail root bending and torsional moments due to MPS and SRB plume interactions were determined. Nozzle power conditions were controlled per pretest nozzle calibrations. Model angle of attack was varied from -4 deg to +4 deg; model angle of sideslip was varied from -4 deg to +4 deg. Reynolds number was varied for certain test conditions and configurations, with the nominal freestream total pressure being 14.69 psia. Plotted force and pressure data are presented.

  19. Heat transfer tests of an 0.006-scale thin-skin space shuttle thermocouple model (41-OTS) in the Langley Research Center unitary plan wind tunnel at M equals 3.7 (IH16)

    NASA Technical Reports Server (NTRS)

    Walstad, D. G.

    1975-01-01

    The results are presented of supersonic heat transfer tests performed on the .006 scale space shuttle vehicle model (41-OTS) in the Langley Research Center Unitary Plan Wind Tunnel. These tests were conducted to parametrically investigate ascent heating of the integrated vehicle and its components. The tests were conducted at a nominal Mach number of 3.7 and Reynolds numbers per foot of 2 and 5 million. The model configurations investigated were the integrated vehicle and each component alone (i.e. orbiter, tank and SRB). All the configurations were run with and without transition strips and through an angle of attack range of 0 deg to minus 5 deg with the exception of the SRB which was tested through an angle of attack range of minus 5 deg to 90 deg. The heat transfer data were obtained from 223 iron constantan thermocouples attached to stainless steel thin-skin areas of the model.

  20. Results of investigations conducted in the LaRC 4-foot unitary plan wind tunnel leg no. 1 using the 0.010-scale 72-OTS model of the space shuttle integrated vehicle (IA94A)

    NASA Technical Reports Server (NTRS)

    Nichols, M. E.

    1976-01-01

    Aero-loads investigations were conducted on the updated configuration-5 space shuttle launch vehicle at Mach numbers 2.50, 3.50, and 4.50. Six-component vehicle forces and moments, base and sting-cavity pressures, elevon hinge moments, wing-root bending and torsion moments, and normal shear force data were obtained. Full simulation of updated vehicle protuberances and attach hardware was employed. Various elevon deflection angles were tested, with two different forward orbiter-to-external-tank attach-strut configurations. The entire vehicle model 72-OTS was supported by means of a balance mounted in the orbiter through its base and suspended from an appropriate sting for the specific tunnel.

  1. Aerodynamic results of a separation effects test on a 0.010-scale model (52-OTS) of the integrated SSV in the AEDC/VKF 40-by-40 inch supersonic wind tunnel A (IA111), volume 1

    NASA Technical Reports Server (NTRS)

    Chee, E.

    1976-01-01

    Graphical data obtained during experimental wind tunnel aerodynamic investigations of a 0.010 scale model (52-OTS) of the integrated space shuttle vehicle was presented. The purpose of this investigation was to obtain data with the solid rocket booster (SRB) in proximity to the orbiter/external tank (O/ET), over a large O/ET initial angle of attack and sideslip range, as well as data on the SRB alone (greatly separated from the O/ET). A captive trajectory system, which supported the SRB, was used with the tunnel primary sector (supporting the O/ET) to obtain grid type separation effects data. One symmetrical SRB model was used interchangeably to obtain right-hand and left-hand SRB data. The entire investigation was conducted at a free-stream Mach number of 4.5 at unit Reynolds number of 3.95 and 5.9 million per foot.

  2. Results of an investigation of the space shuttle integrated vehicle aerodynamic heating characteristics obtained using the 0.0175-scale model 60-OTS in AEDC tunnel A during tests IH41 and IH41A

    NASA Technical Reports Server (NTRS)

    Cummings, J. W.; Dye, W. H.

    1977-01-01

    A thin skin thermocouple test was conducted to obtain heat-transfer data on the space shuttle integrated vehicle during the ascent phase of the flight profile. The test model was the 0.0175-scale thin skin thermocouple model (60-OTS) of the Rockwell International vehicle 5 configuration. The test was conducted at nominal Mach numbers of 2.5, 3.5, 4.5, and 5.5, and a free stream unit Reynolds number of 5 million per ft. Heat transfer data were obtained for angles of attack of 0, + or - 5, and 10 deg and yaw angles of 0, 3, and 6 deg. The integrated vehicle model was tested with the external tank configured with both a smooth ogive nose and an ogive nose with a spherical nose tip (nipple nose). The remainder of the test was conducted with the external tank installed alone in the tunnel.

  3. Aerodynamic results of a separation effects test on a 0.010-scale model (52-OTS) of the integrated SSV in the AEDC/VKF 40-by-40 inch supersonic wind tunnel A (IA111), volume 2

    NASA Technical Reports Server (NTRS)

    Chee, E.

    1976-01-01

    Tabular data obtained during experimental wind tunnel aerodynamic investigations of a 0.010 scale model (52-OTS) of the integrated space shuttle vehicle was presented. The purpose of this investigation was to obtain data with the solid rocket booster (SRB) in proximity to the orbiter/external tank (O/ET), over a large O/ET initial angle of attack and sideslip range, as well as data on the SRB alone (greatly separated from the O/ET). A captive trajectory system, which supported the SRB, was used with the tunnel primary sector (supporting the O/ET) to obtain grid type separation effects data. One symmetrical SRB model was used interchangeably to obtain right-hand and left-hand SRB data. The entire investigation was conducted at free-stream Mach number of 4.5 at unit Reynolds number of 3.95 and 5.9 million per foot.

  4. An experimental determination in Calspan Ludwieg tube of the base environment of the integrated space shuttle vehicle at simulated Mach 4.5 flight conditions (test IH5 of model 19-OTS)

    NASA Technical Reports Server (NTRS)

    Drzewiecki, R. F.; Foust, J. W.

    1976-01-01

    A model test program was conducted to determine heat transfer and pressure distributions in the base region of the space shuttle vehicle during simulated launch trajectory conditions of Mach 4.5 and pressure altitudes between 90,000 and 210,000 feet. Model configurations with and without the solid propellant booster rockets were examined to duplicate pre- and post-staging vehicle geometries. Using short duration flow techniques, a tube wind tunnel provided supersonic flow over the model. Simultaneously, combustion generated exhaust products reproduced the gasdynamic and thermochemical structure of the main vehicle engine plumes. Heat transfer and pressure measurements were made at numerous locations on the base surfaces of the 19-OTS space shuttle model with high response instrumentation. In addition, measurements of base recovery temperature were made indirectly by using dual fine wire and resistance thermometers and by extrapolating heat transfer measurements.

  5. Transposon Mutations in the 5′ End of glnD, the Gene for a Nitrogen Regulatory Sensor, That Suppress the Osmosensitive Phenotype Caused by otsBA Lesions in Escherichia coli

    PubMed Central

    Tøndervik, Anne; Torgersen, Haakon R.; Botnmark, Hans K.; Strøm, Arne R.

    2006-01-01

    GlnD of Escherichia coli is a bifunctional signal-transducing enzyme (102.4 kDa) which uridylylates the allosteric regulatory protein PII and deuridylylates PII-UMP in response to growth with nitrogen excess or limitation, respectively. GlnD catalyzes these reactions in response to high or low levels of cytoplasmic glutamine, respectively, and indirectly directs the expression of nitrogen-regulated genes, e.g., the glnK-amtB operon. We report that chromosomal mini-Tn10 insertions situated after nucleotide number 997 or 1075 of glnD partially suppressed the osmosensitive phenotype of ΔotsBA or otsA::Tn10 mutations (defective osmoregulatory trehalose synthesis). Strains carrying these glnD::mini-Tn10 mutations either completely repressed the expression of trp::(glnKp-lacZ) or induced this reporter system to nearly 60% of the wild-type glnD level in response to nitrogen availability, an essentially normal response. This was in contrast to the much-studied glnD99::Tn10 mutation, which carries its insertion in the 3′ end of the gene, causes a complete repression of glnKp-lacZ expression under all growth conditions, and also confers leaky glutamine auxotrophy. When expressed from the Pm promoter in plasmid constructs, the present glnD mutations produced proteins with an apparent mass of 39 or 42 kDa. These proteins were deduced to comprise 344 or 370 N-terminal residues, respectively, harboring the known nucleotidyltransferase domain of GlnD, plus a common C-terminal addition of 12 residues encoded by IS10. They lacked three other domains of GlnD. Apparently, the transferase domain by itself enabled the cells to catalyze the uridylylation reaction and direct nitrogen-regulated gene expression. Our data indicate that there exists a link between osmotic stress and the nitrogen response. PMID:16740928

  6. Results of aeroheating DFI and ET design-data test on a 0.0175-scale model 60-OTS conducted in the Von Karman Gas Dynamics Facility (VKF) 40" supersonic and the 50" hypersonic wind tunnels A and C

    NASA Technical Reports Server (NTRS)

    Marroquin, J.; Leef, C. R.

    1985-01-01

    The 0.0175-scale thin-skin thermocouple Model 60-OTS was tested in the von Karman Gas Facility 40-inch continuous flow supersonic tunnel A and the VKF Tunnel C. Testing was conducted at Mach numbers 2.25 to 4.0, and Reynolds numbers from 0.4 x 10 to the 6th power/ft to 6.6 x 10 to the 6th power/ft. Angle of attack range was from -5.0 to +5.0 degrees, and angle of sideslip range was from -6 to +6 degrees. The primary objective of this test was to provide a valid base for the external tank (ET) and solid rocket booster (SRB) heating prediction methodology for ascent flight by taking heating data at Development Flight Instrumentation (DFI) locations for flight conditions simulating STS-1 through -4. A second objective was to obtain additional aeroheating data to support potential reduction of the thermal protection system (TPS) on the ET. The third phase of the test was funded and conducted by NASA/MSFC for the purpose of establishing confidence in the data base from the lower temperature tunnel A.

  7. Aerodynamic results of a separation effects test on a 0.01-scale model (52-OTS) of integrated SSV in the AEDC/VKF 40-by-40 inch supersonic wind tunnel A, volume 1

    NASA Technical Reports Server (NTRS)

    Campbell, J. H., II

    1975-01-01

    Experimental aerodynamic investigations were conducted, during the period July 18-19, 1974, in the AEDC/VKF Tunnel A facility on a 0.01-scale model (52-OTS) of the integrated space shuttle vehicle, including only one SRB. The purpose of the investigation was to obtain data for close-in proximity (SRB to orbiter/tank) effects with the orbiter/tank combination at relatively high alpha and beta attitudes, and with the SRB separation motors off. The AEDC Captive Trajectory System (CTS), which supported the SRB, was used in conjunction with the tunnel primary sector (supporting the orbiter/tank) to obtain grid type separation effects data. The one symmetrical SRB model was used interchangeably to obtain both right-hand and left-hand SRB data. Free-stream data were also obtained for the orbiter/tank and for the SRB. This data was used to provide baselines for proximity effects. The entire investigation was conducted at a free-stream Mach number of 4.5 with unit Reynolds number ranging from 4.0 to 6.5 million per foot.

  8. A new report of Nocardiopsis valliformis strain OT1 from alkaline Lonar crater of India and its use in synthesis of silver nanoparticles with special reference to evaluation of antibacterial activity and cytotoxicity.

    PubMed

    Rathod, Dnyaneshwar; Golinska, Patrycja; Wypij, Magdalena; Dahm, Hanna; Rai, Mahendra

    2016-10-01

    The authors report the biological synthesis of silver nanoparticles (AgNPs) by alkaliphilic actinobacterium Nocardiopsis valliformis OT1 strain isolated for the first time from Lonar crater, India. The primary detection of silver NPs formation was made by colour change from colourless to dark brown and confirmed by UV-Vis spectrum of AgNPs at 423 nm, specific for AgNPs. Further, AgNPs were characterized by nanoparticle tracking analysis, Zeta sizer, Fourier transform infrared spectroscopy (FTIR) and transmission electron microscopy (TEM) analyses. FTIR analysis showed the presence of proteins as capping agent. TEM analysis revealed the formation of spherical and polydispersed AgNPs within the size range of 5-50 nm. The antimicrobial activity of silver nanoparticles against Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis was evaluated. The AgNPs showed the maximum antibacterial activity against B. subtilis (Gram positive) and the minimum against E. coli (Gram negative). The minimal inhibitory concentration values of AgNPs for the tested bacteria were found to be in the range of 30-80 µg/mL. The AgNPs demonstrated higher antibacterial activity against all the bacteria tested as compared with the commercially available antibiotics. The cytotoxicity of biosynthesized AgNPs against in vitro human cervical cancer cell line (HeLa) demonstrated a dose-response activity. The IC50 value was found to be 100 µg/mL of AgNPs against cancer HeLa cell line.

  9. Transposon mutations in the 5' end of glnD, the gene for a nitrogen regulatory sensor, that suppress the osmosensitive phenotype caused by otsBA lesions in Escherichia coli.

    PubMed

    Tøndervik, Anne; Torgersen, Haakon R; Botnmark, Hans K; Strøm, Arne R

    2006-06-01

    GlnD of Escherichia coli is a bifunctional signal-transducing enzyme (102.4 kDa) which uridylylates the allosteric regulatory protein PII and deuridylylates PII-UMP in response to growth with nitrogen excess or limitation, respectively. GlnD catalyzes these reactions in response to high or low levels of cytoplasmic glutamine, respectively, and indirectly directs the expression of nitrogen-regulated genes, e.g., the glnK-amtB operon. We report that chromosomal mini-Tn10 insertions situated after nucleotide number 997 or 1075 of glnD partially suppressed the osmosensitive phenotype of DeltaotsBA or otsA::Tn10 mutations (defective osmoregulatory trehalose synthesis). Strains carrying these glnD::mini-Tn10 mutations either completely repressed the expression of trp::(glnKp-lacZ) or induced this reporter system to nearly 60% of the wild-type glnD level in response to nitrogen availability, an essentially normal response. This was in contrast to the much-studied glnD99::Tn10 mutation, which carries its insertion in the 3' end of the gene, causes a complete repression of glnKp-lacZ expression under all growth conditions, and also confers leaky glutamine auxotrophy. When expressed from the Pm promoter in plasmid constructs, the present glnD mutations produced proteins with an apparent mass of 39 or 42 kDa. These proteins were deduced to comprise 344 or 370 N-terminal residues, respectively, harboring the known nucleotidyltransferase domain of GlnD, plus a common C-terminal addition of 12 residues encoded by IS10. They lacked three other domains of GlnD. Apparently, the transferase domain by itself enabled the cells to catalyze the uridylylation reaction and direct nitrogen-regulated gene expression. Our data indicate that there exists a link between osmotic stress and the nitrogen response. PMID:16740928

  10. A new report of Nocardiopsis valliformis strain OT1 from alkaline Lonar crater of India and its use in synthesis of silver nanoparticles with special reference to evaluation of antibacterial activity and cytotoxicity.

    PubMed

    Rathod, Dnyaneshwar; Golinska, Patrycja; Wypij, Magdalena; Dahm, Hanna; Rai, Mahendra

    2016-10-01

    The authors report the biological synthesis of silver nanoparticles (AgNPs) by alkaliphilic actinobacterium Nocardiopsis valliformis OT1 strain isolated for the first time from Lonar crater, India. The primary detection of silver NPs formation was made by colour change from colourless to dark brown and confirmed by UV-Vis spectrum of AgNPs at 423 nm, specific for AgNPs. Further, AgNPs were characterized by nanoparticle tracking analysis, Zeta sizer, Fourier transform infrared spectroscopy (FTIR) and transmission electron microscopy (TEM) analyses. FTIR analysis showed the presence of proteins as capping agent. TEM analysis revealed the formation of spherical and polydispersed AgNPs within the size range of 5-50 nm. The antimicrobial activity of silver nanoparticles against Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis was evaluated. The AgNPs showed the maximum antibacterial activity against B. subtilis (Gram positive) and the minimum against E. coli (Gram negative). The minimal inhibitory concentration values of AgNPs for the tested bacteria were found to be in the range of 30-80 µg/mL. The AgNPs demonstrated higher antibacterial activity against all the bacteria tested as compared with the commercially available antibiotics. The cytotoxicity of biosynthesized AgNPs against in vitro human cervical cancer cell line (HeLa) demonstrated a dose-response activity. The IC50 value was found to be 100 µg/mL of AgNPs against cancer HeLa cell line. PMID:27278909

  11. Optical Monitors and OT/GRB analyses

    NASA Astrophysics Data System (ADS)

    Hudec, R.; Krizek, M.

    2005-07-01

    The Optical Monitors, despite of lower detection limits, are still valuable for detection of prompt real-time and (hypothetical) pre-burst optical emission of gamma-ray bursts. We refer on the ongoing project at the Astronomical Institute in Ondřejov based on digitized data from the photographic EN network.

  12. Results of a M = 5.3 heat transfer test of the integrated vehicle using phase-change paint techniques on the 0.0175-scale model 56-OTS in the NASA/Ames Research Center 3.5-foot hypersonic wind tunnel

    NASA Technical Reports Server (NTRS)

    Marroquin, J.

    1985-01-01

    An experimental investigation was performed in the NASA/Ames Research Center 3.5-foot Hypersonic Wind Tunnel to obtain supersonic heat-distribution data in areas between the orbiter and external tank using phase-change paint techniques. The tests used Novamide SSV Model 56-OTS in the first and second-stage ascent configurations. Data were obtained at a nominal Mach number of 5.3 and a Reynolds number per foot of 5 x 10 to the 6th power with angles of attack of 0 deg, +/- 5 deg, and sideslip angles of 0 deg and +/- 5 deg.

  13. Identity of Innovative Multifunctional Material Manufacturing Business in Latvia / Inovatīvu Daudzfunkcionālo Materiālu Ražotāju Identitāte Latvijā

    NASA Astrophysics Data System (ADS)

    Geipele, I.; Staube, T.; Ciemleja, G.; Zeltins, N.; Ekmanis, Yu.

    2015-08-01

    The publication comprises the results from the practical scientific investigation to define the profile and distinctions of the Latvian innovative multifunctional material manufacturing industry. The research is carried out by a holistic approach, including expert interviews, qualitative analysis of the official register data, practical survey, and financial data analysis. The paper seeks to give the detailed data on a technological profile of the mentioned representative companies, if there is a synergy or tough competition in the Latvian market. The current research is topical, because it is unique and does not have analogues in Latvia, and the research is timely due to correspondence with recently stated Research and Innovation Strategies for Smart Specialisation in Latvia. The main findings are associated with recognition of the factors that make impact on commercialisation of the finished goods, obtained financial results and planned directions of development of the respondent companies. Publikācija sniedz veiktā zinātniski praktiskā pētījuma rezultātus, kura ietvaros tika noteikts Latvijas inovatīvu daudzfunkcionālo materiālu ražotāju darbības profils un identitāte. Pētījumā tika izmantota kompleksās analīzes metode, kas ietvēra ekspertu intervijas, uzņēmumu oficiāli reģistrēto datu kvalitatīvo analīzi, zinātniski praktisko aptauju, kā arī pētāmo uzņēmumu finanšu darbības rezultātu analīzi. Zinātniskais darbs sniedz izsmeļošu informāciju par mērķa uzņēmumu tehnoloģisko profilu un pēta, vai Latvijas mērķa ražošanas nozarē ir sinerģijas vai sīvās konkurences pazīmes. Šim zinātniskajam pētījumam ir noteikta aktualitāte saskaņā ar tā unikalitāti Latvijas valsts mērogā un atbilstība Viedās specializācijas stratēģijai. Pētījuma galvenie secinājumi ir saistīti ar uzņēmumu produkcijas komercializācijas ietekmējošo faktoru apzināšanu, sasniegtajiem finansiālajiem rezultātiem un pl

  14. 12 CFR 563.143 - Must I file with OTS?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... depends on whether you and your proposed capital distribution fall within certain criteria. (a... common or preferred stock or retire any part of debt instruments such as notes or debentures included in... proposed capital distribution meet any of the criteria listed in paragraphs (a) and (b) of this...

  15. 12 CFR 510.5 - Release of unpublished OTS information.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Act, see 5 U.S.C. 552, and 31 CFR 1.1-1.6. (b) Purpose. The purposes of this section are: (1) To... Department regulations, 31 CFR 1.7. With certain exceptions, the regulations in 31 CFR 1.7 provide for.... Examples of unpublished information include: (i) Information in the memory of a current or former...

  16. 12 CFR 510.5 - Release of unpublished OTS information.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... disclosed under the Freedom of Information Act, see 5 U.S.C. 552, and 31 CFR 1.1-1.6; and (iv) Requests for... charged fees in accordance with Treasury Department regulations, 31 CFR 1.7. With certain exceptions, the regulations in 31 CFR 1.7 provide for recovery of the full direct costs of searching, reviewing,...

  17. 12 CFR 574.7 - Determination by the OTS.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... financial condition of the acquiring person is such as might jeopardize the financial stability of the... frames: (1) On the business day prior to the date of consummation of the acquisition, the chief financial... material adverse events or material adverse changes have occurred with respect to the financial...

  18. Complete genome sequence of Haliscomenobacter hydrossis type strain (OT)

    SciTech Connect

    Daligault, Hajnalka E.; Lapidus, Alla L.; Zeytun, Ahmet; Nolan, Matt; Lucas, Susan; Glavina Del Rio, Tijana; Tice, Hope; Cheng, Jan-Fang; Tapia, Roxanne; Han, Cliff; Goodwin, Lynne A.; Pitluck, Sam; Liolios, Konstantinos; Pagani, Ioanna; Ivanova, N; Huntemann, Marcel; Mavromatis, K; Mikhailova, Natalia; Pati, Amrita; Chen, Amy; Palaniappan, Krishna; Land, Miriam L; Hauser, Loren John; Brambilla, Evelyne-Marie; Rohde, Manfred; Verbarg, Susanne; Goker, Markus; Bristow, James; Eisen, Jonathan; Markowitz, Victor; Hugenholtz, Philip; Kyrpides, Nikos C; Klenk, Hans-Peter; Woyke, Tanja

    2011-01-01

    Haliscomenobacter hydrossis van Veen et al. 1973 is the type species of the genus Halisco- menobacter, which belongs to order 'Sphingobacteriales'. The species is of interest because of its isolated phylogenetic location in the tree of life, especially the so far genomically un- charted part of it, and because the organism grows in a thin, hardly visible hyaline sheath. Members of the species were isolated from fresh water of lakes and from ditch water. The genome of H. hydrossis is the first completed genome sequence reported from a member of the family 'Saprospiraceae'. The 8,771,651 bp long genome with its three plasmids of 92 kbp, 144 kbp and 164 kbp length contains 6,848 protein-coding and 60 RNA genes, and is a part of the Genomic Encyclopedia of Bacteria and Archaea project.

  19. 12 CFR 555.310 - How do I notify OTS?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... at least 30 days before you establish a transactional website. The notice must do three things: (1... operational. (3) List a contact familiar with the deployment, operation, and security of the transactional...

  20. OTS cash disbursement process for HANDI 2000 business management system

    SciTech Connect

    Wilson, D.

    1998-08-24

    Payments by check are disbursed either through the payroll system or the Accounts Payable Master. Payments to persons with a valid employee number are processed through the payroll system. Payments to persons without valid employee number are processed through APM.

  1. A Round Trip Ticket from Goal Development ot Boardroom.

    ERIC Educational Resources Information Center

    Peper, John B.

    The speaker comments on a goal development process that has been used in the Milwaukee Public Schools for planning and implementing policy. Improvement of attendance was the goal selected for use in illustrating the model. (Author/IRT)

  2. OT1_rmushotz_1: Determining the Bolometric Luminosity of AGN

    NASA Astrophysics Data System (ADS)

    Mushotzky, R.

    2010-07-01

    Determining the bolometric luminosities of AGN is key to understanding their evolution. Uncertainties in the total radiation from AGN translate into uncertainties in their lifetimes, Eddington ratios, mass accretion rates, the form of their radiation, and the predicted black hole spin. However, we still have major problems in measuring this critical quantity. AGN and their host galaxies emit a large fraction of their light in the MIR to FIR, but the origin of this radiation and the connection to the AGN are not well understood. It is not clear whether this radiation is associated with the AGN or with star formation in the galaxy. We propose to use Herschel's unique capabilities to establish the properties of the Swift-BAT all sky sample of local AGN selected at 15-195 keV. We will measure the MIR to FIR (65-500 microns) properties of a complete low-redshift sample (309 objects at z<0.05). The Swift-BAT survey is the least biased all sky survey for AGN with respect to host galaxy properties and obscuration in the line-of-sight, and thus it is superior to optical, IR, or radio surveys for understanding the the nuclear component of the MIR to FIR radiation from active galaxies. The low redshift of our sample, the uniformity of selection, and the large amount of parallel data which have already been obtained (Spitzer, optical, and X-ray spectra, and optical and UV imaging) will allow the most precise determination of the physical origin (AGN versus star formation) of the light. The low redshifts allow the best possible angular resolution for spatially separating star-formation and nuclear components, while only requiring short Herschel exposures. The Herschel BAT survey will provide a comprehensive database for determining the bolometric light of AGN and will be an invaluable reference sample for analyzing higher redshift AGN. It will be a powerful resource for many years to come. We will make it available in a comprehensive and accessible form as rapidly as possible.

  3. Treatability study Number PDC-1-O-T. Final report

    SciTech Connect

    1998-04-22

    Los Alamos National Laboratory provided treatability study samples from four waste streams, designated Stream {number_sign}1, Stream {number_sign}3, Stream {number_sign}6, and Stream {number_sign}7. Stream {number_sign}1 consisted of one 55-gallon drum of personal protective equipment (PPE), rags, and neutralizing agent (bicarbonate) generated during the cleanup of a sodium dichromate solution spill. Stream {number_sign}3 was one 55-gallon drum of paper, rags, lab utensils, tools, and tape from the decontamination of a glovebox. The sample of Stream {number_sign}6 was packaged in three 30-gallon drums and a 100 ft{sup 3} wooden box. It consisted of plastic sheeting, PPE, and paper generated from the cleanup of mock explosive (barium nitrate) from depleted uranium parts. Stream {number_sign}7 was scrap metal (copper, stainless and carbon steel joined with silver solder) from the disassembly of gas manifolds. The objective of the treatability study is to determine: (1) whether the Perma-Fix stabilization/solidification process can treat the waste sample to meet Land Disposal Restrictions and the Waste Acceptance Criteria for LANL Technical Area 54, Area G, and (2) optimum loading and resulting weight and volume of finished waste form. The stabilized waste was mixed into grout that had been poured into a lined drum. After each original container of waste was processed, the liner was closed and a new liner was placed in the same drum on top of the previous closed liner. This allowed an overall reduction in waste volume but kept waste segregated to minimize the amount of rework in case analytical results indicated any batch did not meet treatment standards. Samples of treated waste from each waste stream were analyzed by Perma-Fix Analytical Services to get a preliminary approximation of TCLP metals. Splits of these samples were sent to American Environmental Network`s mixed waste analytical lab in Cary, NC for confirmation analysis. Results were all below applicable limits.

  4. 12 CFR 574.7 - Determination by the OTS.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... company form is subject to provisions relating to its mutual to stock conversion imposed by l2 CFR 563b.3... determined using the standards set forth at 12 CFR 211.24(c)(1)(ii). (d) Notice criteria. In making its... conspiracy to monopolize or to attempt to monopolize the savings and loan business in any part of the...

  5. OT2_baltieri_5: Star formation in proto-clusters

    NASA Astrophysics Data System (ADS)

    Altieri, B.

    2011-09-01

    Massive clusters of galaxies have been found to date from as early as 3-4 billion years after the Big Bang. Cosmological simulations using the current cold dark matter model predict that these systems should descend from 'proto-clusters' - early overdensities of massive galaxies that merge hierarchically to form a cluster. These protocluster regions themselves are built up hierarchically and so are expected to contain extremely massive galaxies, progenitors of the quiescent behemoths observed in cores of the present day massive galaxy clusters. Observational evidence for this picture, however, is sparse because high-redshift proto-clusters are rare and difficult to observe. Here we propose to probe with Herschel SPIRE the very beginning of the cluster and massive galaxies formation process by observing 5 proto-clusters at 3

  6. (/sup 3/H)-(Thr4,Gly7)OT: a highly selective ligand for central and peripheral OT receptors

    SciTech Connect

    Elands, J.; Barberis, C.; Jard, S.

    1988-01-01

    Oxytocin receptors in rat hippocampal synaptic plasma membranes were compared with mammary gland and uterine oxytocin receptors. For this purpose, a highly specific oxytocic agonist (Thr4,Gly7)oxytocin was tritiated. We demonstrated that this ligand labels oxytocin receptors selectively. Scatchard analyses revealed a high affinity for all the oxytocin receptors investigated, with equilibrium dissociation constants between 1.0 and 2.0 nM. Binding appeared to take place at a single population of receptor sites. Competition experiments confirmed the high affinity of arginine vasopressin for hippocampal oxytocin receptors but also revealed that mammary gland and uterine oxytocin receptors do not discriminate more efficiently between oxytocin and arginine vasopressin. This lack in specificity is not affected by applying different concentrations of Mg ions.

  7. WHY DO THE ACUTE BEHAVIORAL EFFECTS OT TOLUENE IN RATS DEPEND ON THE ROUTE OF EXPOSURE?

    EPA Science Inventory

    Despite evidence suggesting that the acute effects of organic solvents are related to their concentration in the brain, we have observed route-dependent differences in the acute behavioral effects of toluene. Whereas inhaled toluene disrupts the performance of rats on a visual si...

  8. 78 FR 76721 - Removal of Transferred OTS Regulations Regarding Recordkeeping and Confirmation Requirements for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-19

    ... Final Rule conforms the interpretations of that exception, as discussed below. \\3\\ 78 FR 54403, 54408... Transaction Exception.\\7\\ \\6\\ Id. \\7\\ 78 FR 54406. In addition, the Proposed Rule included a measure...

  9. OT1_cvastel_2: Deuterated water chemistry towards high-mass star-forming regions.

    NASA Astrophysics Data System (ADS)

    Vastel, C.

    2010-07-01

    Observations of the HDO molecules are an important complement for studies of water, since they give strong constraints on the formation processes: grain surfaces versus gas-phase chemistry through energetic process as shocks. HIFI observations of multiple transitions of HDO in SgrB2(M) combined with ground-based observations allowed for the first time the determination of its abundance throughout the envelope. In the framework of the PRISMAS Key Program, a large sample of high-mass star-forming regions have been observed with the detection of many species in their line of sight. The HDO (111-000) fundamental transition has also been detected in absorption at the velocity of the hot core towards the 2 sources that have been observed so far, probably tracing the colder envelope in its surrounding. We propose to observe higher energy level HDO transitions towards a sample of three compact HII regions that will be targeted by the PRISMAS Key Program (G34.3+0.1, W33A, W49N) in order to perform a full modeling from the hot core through the envelope using a spherical Monte Carlo radiative transfer code, RATRAN, which takes into account radiative pumping by continuum emission from dust. We will use for an optimum accuracy of the modeling the HDO and D2O collision rates with H2, recently computed within our group, that are not available in the public so far. This study will hopefully give strong constrains on the formation processes of water, combining the proposed observations with published or soon to be published high resolution H2O observations with HIFI towards the same sources.

  10. I Have Students with Physical and Motor Problems: How Can an APE, OT, or PT Help?

    ERIC Educational Resources Information Center

    Silliman-French, Lisa; Candler, Catherine; French, Ron; Hamilton, Merry Lynne

    2007-01-01

    Majority of students with mental and/or motor impairments are frequently placed in general physical education classes. However, these students often are unable to attain the expected outcomes in these general settings. Based on the experience of the authors, general physical educators often attempt to modify their goals, objectives, teaching…

  11. Ursa Major: ot losya do medvedya %t Ursa Major: from elk to bear

    NASA Astrophysics Data System (ADS)

    Lushnikova, A. V.

    In the article material from various cultural and linguistic sources (Indo-European - Slavic, Indo-Iranian; Uralo-Altaic, Tungus, Ancient Egyptian, Babylonian) is used in order to trace up the chronology of the designation of Ursa Majopr, a constellation which has been playing an important role for people different regions since ancient times. It was used for observing of the visible yearly motion of the Sun, for working out seasonal changes; being a circumpolar and non-hiding behind the horizon it has been perceived as a symbol of immortality, its peculiar positional change during a year lay down in plot of the Uralo-Siberian myths about a cosmic hunt for the Elk, myths about deluge. Data from Uralo-Siberian mythology are analyzed. Designations of Ursa Major in the form of a horned hoofed animal such as elk, deer, cow (Uralo-Altaic, Tungus, Slavic, Indo-Iranian languages; Ancient Greece, Crete, Ancient Egypt) and connected with it (or derived from it) denominations and images of "enclosed space" - "vehicle for travelling and carrying goods" (a wagon, a boat) - "instrument for hunting-fishing, a ritual thing" - "household construction" are taken in consideration. The conclusion is made that the transition of the Ursa Major designation from elk to bear could follow the general tendency to shift from so-called matriarchy to patriarchy, to substitute female deities with male ones, which was reflected "in the rise" of the predatory animal cults (not earlier than II mil. B.C.). To prove this, lexical examples of resemblance and coincidences in designation of homed hoofed (elk, deer) and predatory (bear, wolf) animals should be analyzed. Such a goal-directed investigation of the chronology of Ursa Major designations has never been carried out.

  12. ICT and OTs: a model of information and communication technology acceptance and utilisation by occupational therapists.

    PubMed

    Schaper, Louise K; Pervan, Graham P

    2007-06-01

    There is evidence to suggest that health professionals are reluctant to accept and utilise information and communication technologies (ICT) and concern is growing within health informatics research that this is contributing to the lag in adoption and utilisation of ICT across the health sector. Technology acceptance research within the field of information systems has been limited in its application to health and there is a concurrent need to develop and gain empirical support for models of technology acceptance within health and to examine acceptance and utilisation issues amongst health professionals to improve the success of information system implementation in this arena. This paper outlines a project that examines ICT acceptance and utilisation by Australian occupational therapists. It describes the theoretical basis behind the development of a research model and the methodology being employed to empirically validate the model using substantial quantitative, qualitative and longitudinal data. Preliminary results from Phase II of the study are presented. The theoretical significance of this work is that it uses a thoroughly constructed research model, with potentially the largest sample size ever tested, to extend technology acceptance research into the health sector.

  13. Local and Global Magnetic Fields of Late-Type Dwarfs OT Ser and YZ CMi

    NASA Astrophysics Data System (ADS)

    Bychkov, V. D.; Bychkova, L. V.; Madej, J.; Panferov, A. A.

    2015-04-01

    Differential rotation is the primary energy source for generation of local magnetic fields in the atmospheres of late-type stars (Moss et al. 1995). Moreover, the colder a star, the greater the effect, which was confirmed by observations. For instance, Saar (1988) measured the surface magnetic fields of late-type stars using the integral method Robinson (1980) and drew attention to the fact that the average magnetic field in the spots reaches the values of 1500 G for the G-type dwarfs, 2500 G for the K dwarfs, and 3500 G for the M-dwarf stars. The fraction of the surface of a star covered by spots also increases towards the latest spectral types. These well-known observational facts were explained by theoretical models, such as the α2 mechanism, for instance (Moss et al. 1995). Late-type dwarfs exhibit periodic eruptions resulting from the field line reconnection of newly generated local magnetic fields. This feature is observed in G, K, and M dwarfs, constituting 95%of all the stars in our Galaxy. The most prominent are the field reconnections in the so-called flare stars, which are the M dwarfs. This is understandable, since the peak flux of M dwarfs is placed in the red and infrared regions of the spectrum, whereas a flash has the maximum emission in the violet spectral region. Analysis of long-term photometric observations revealed that, on the average, energy of flares was found constant over a long time period for each flare star. That is to say, this conclusion implies that the power of the local magnetic field generator remains constant at this stage of evolution of stars.

  14. Lateral sellar compartment O.T. (cavernous sinus): history, anatomy, terminology.

    PubMed

    Parkinson, D

    1998-08-01

    Claudios Galen (119-199 a.d.) dissected lower animals with parasellar carotid retia bathed in venous blood and transposed his findings to human anatomy. Andreas Vesalius (1514-1564) corrected most of Galen's errors but apparently never looked into this small, extradural compartment, nor, apparently, did Winslow (Exposition Anatomique de la Structure du Corps Humain. London: N. Prevast, 1734), who christened it the "cavernous sinus," (CS) presumably thinking that it would resemble the corpora cavernosa of the penis. Multiple surgical explorations, gross dissections, microscopic views, and vascular casts from early fetuses to an 81 year old have been examined and reviewed. The CS is not a dural sinus nor is it cavernous. The compartment is extradural, and the venous structures contained within consist of a greatly variable plexus of extremely thin-walled veins. The name, CS, is a barrier to the understanding of the structure and function of this extradural anatomical jewel box, which contains fat, myelinated and nonmyelinated nerves, arteries, and a plexus of veins. It is proposed that this name be changed, because it is inaccurate and misleading. The replacement should leave no doubt about its meaning. The lateral sellar compartment is descriptive and accurate. The veins within are a parasellar plexus. PMID:9713986

  15. OT2_dardila_2: PACS Photometry of Transiting-Planet Systems with Warm Debris Disks

    NASA Astrophysics Data System (ADS)

    Ardila, D.

    2011-09-01

    Dust in debris disks is produced by colliding or evaporating planetesimals, the remnant of the planet formation process. Warm dust disks, known by their emission at =<24 mic, are rare (4% of FGK main-sequence stars), and specially interesting because they trace material in the region likely to host terrestrial planets, where the dust has very short dynamical lifetimes. Dust in this region comes from very recent asteroidal collisions, migrating Kuiper Belt planetesimals, or migrating dust. NASA's Kepler mission has just released a list of 1235 candidate transiting planets, and in parallel, the Wide-Field Infrared Survey Explorer (WISE) has just completed a sensitive all-sky mapping in the 3.4, 4.6, 12, and 22 micron bands. By cross-identifying the WISE sources with Kepler candidates as well as with other transiting planetary systems we have identified 21 transiting planet hosts with previously unknown warm debris disks. We propose Herschel/PACS 100 and 160 micron photometry of this sample, to determine whether the warm dust in these systems represents stochastic outbursts of local dust production, or simply the Wien side of emission from a cold outer dust belt. These data will allow us to put constraints in the dust temperature and infrared luminosity of these systems, allowing them to be understood in the context of other debris disks and disk evolution theory. This program represents a unique opportunity to exploit the synergy between three great space facilities: Herschel, Kepler, and WISE. The transiting planet sample hosts will remain among the most studied group of stars for the years to come, and our knowledge of their planetary architecture will remain incomplete if we do not understand the characteristics of their debris disks.

  16. OT2_dteyssie_2: Unveiling the water puzzle in cold PDRs: The Horsehead case

    NASA Astrophysics Data System (ADS)

    Teyssier, D.

    2011-09-01

    While Herschel observations of water vapour in warm regions (T_gas >> 100 K) driven by energetic processes (outfows, shocks, hot cores, etc.) have confirmed large abundances of water vapour (~10^-5), the inferred gas phase H2O abundance in dark clouds is very low, ~10^-10. A detailed balance between freeze-out, ice-mantle desorption and gas-phase chemistry is required to explain this orders-of-magnitude abundance difference. The Horsehead nebula is particularly well-suited to investigate grain surface chemistry in a UV irradiated environment. Its relatively low UV illumination (~60 in Draine units) implies low dust grain temperatures, from T_dust=30K in the PDR to T_dust=20K deeper inside the cloud. Therefore, the release of the grain mantle products into the gas phase, water vapour in particular, is dominated by UV-induced photo-desorption and not by thermal evaporation (as in other warm PDRs such as the Orion Bar). Besides, owing to its simple edge-on geometry, the Horsehead is very close to the prototypical kind of source needed to serve as model benchmark. A relatively low water vapour beam-averaged abundances (~5x10^-9) was derived from a single position of the o-H2O ground-state line towards the so-called "IR peak" of the Horsehead PDR. However a detailed comparison with sophisticated PDR models including gas and grain chemistry is not possible due to our complete ignorance about the true H2O spatial distribution as one moves from the UV-illuminated cloud surface to the inner shielded cloud. We propose here to map with HIFI the o-H2O 557 GHz line on a cut across the PDR front, and extract the spatial information required to constrain the role of water freeze-out and water ice photodesorption as a function of cloud depth.

  17. OT1_briaz_4: Disk masses for ~10 Myr old brown dwarf disks

    NASA Astrophysics Data System (ADS)

    Riaz, B.

    2010-07-01

    We request SPIRE 200-500mu observations for two brown dwarf disks, 2MASSW J1207334-393254 (2M1207) and SSSPM J1102-3431 (SSSPM 1102), in the TW Hydrae Association (TWA). From our previous Spitzer observations, we had confirmed excess emission at wavelengths of ~5-38mu for both of these sources. With the SPIRE observations, we can probe the optically thin dust in the outer cooler regions of these disks. We have performed radiative transfer modeling for these systems. With the available mid-infrared observations, we find high degeneracies for the disk mass and outer disk radius estimates. Observations at far-infrared wavelengths can help constrain the model fits and obtain better estimates for the disk mass and outer radius for these disks. At an age of ~10 Myr, 2M1207 and SSSPM1102 are the oldest known brown dwarf disks. It is thus important to estimate the disk masses for these older disks, and to understand, in comparison with the younger Taurus systems, if brown dwarf disk masses show any decline with the age of the system. We also request PACS 70mu photometry for another candidate brown dwarf disk in the TWA, 2MASSW J1139511-315921 (2M1139). This object shows an excess emission at 24mu, but none at shorter wavelengths. With 70mu observations, we can confirm if the disk flares up at longer wavelengths. If the presence of such a disk is confirmed for 2M1139, then this would be the first transition disk detected among the sub-stellar members of TWA.

  18. 12 CFR 502.15 - How does OTS determine my size component?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... calculate your size component: If your total assets are: . . . Over—* Column A But not over— Column B Your size component is: This amount—Base assessment amount Column C Plus—Marginal rate Column D Of assets over—Class floor Column E 0 $67 million C1 D1 0. $67 million 215 million C2 D2 $67 million. 215...

  19. 12 CFR 502.15 - How does OTS determine my size component?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... calculate your size component: If your total assets are: . . . Over—* Column A But not over— Column B Your size component is: This amount—Base assessment amount Column C Plus—Marginal rate Column D Of assets over—Class floor Column E 0 $67 million C1 D1 0. $67 million 215 million C2 D2 $67 million. 215...

  20. OT1_rpaladin_1: PACS and SPIRE observations of Galactic anomalous emission sources.

    NASA Astrophysics Data System (ADS)

    Paladini, R.

    2010-07-01

    Despite the increasing evidence that the anomalous emission is a new physical mechanism acting in the diffuse interstellar medium, the nature and distribution of this component remains elusive. The currently most favored models attribute the observed microwave excess to rotating very small dust grains (PAHs and VSGs). Nonetheless, the infrared properties of the sources which, to date, are known to exhibit this type of emission are very poorly known mostly due to the limited angular resolution and frequency coverage of DIRBE and IRAS data. We propose HERSCHEL PACS and SPIRE mapping of three Galactic anomalous emission sources (LDN 1780, LDN 675 and LDN 1111). This data, when combined with ancillary NIR and mid-IR data of comparable angular resolution (mainly from Spitzer), and coupled with available dust models, will allow to set tight constraints on the radiation field in the emitting sources as well as in their immediate surroundings. Such constraints, in turn, will allow to estimate the abundances of PAHs, VSGs and BGs, hence to shed light on the potential link between these dust populations and the observed microwave excess.

  1. OT2_cmccoey_3: Evolution of water emission in intermediate mass YSOs

    NASA Astrophysics Data System (ADS)

    McCoey, C.

    2011-09-01

    Intermediate mass YSOs are studied as part of the WISH KP. We find our source sample is too limited to identify properties associated with stellar evolution. With this proposal we ask for time to observe two more intermediate mass YSOs with HIFI and PACS. When looking at line profiles among or source set a confused picture appears. NGC 7129 FIRS 2 and LDN 1641 are both Class 0 YSOs but have very different line profiles. Similarly, the Vela sources are both Class I sources and even have almost the same bolometric luminosity but display very different spectra. It is noteworthy that line profiles of the Class 0 NGC 7129 FIRS 2 are very similar to the line profiles of the Class I Vela IRS 17. Furthermore, the most evolved source, AFGL 490, is most similar, albeit brighter, to one of the least evolved sources, LDN 1641. We require more observations in order to assess if there is any relation in line profile with evolutionary state. We ask for time to observe the Class 0 intermediate YSO, Cep E-mm, and the Class I/II sources, LkHa 224.

  2. OT2_ctibbs_1: Exploring the role of CII in current Spinning Dust Models

    NASA Astrophysics Data System (ADS)

    Tibbs, C.

    2011-09-01

    We propose HIFI observations of the CII fine structure line at 158 micron (1.9THz) in 22 pointings distributed across four Galactic anomalous emission regions (the Perseus cloud, LDN 1780, LDN 675 and LDN 1111). The currently favoured explanation for the observed anomalous microwave emission is that of electric dipole radiation from rapidly rotating small dust grains (PAHs and/or VSGs), commonly referred to as spinning dust. Although this hypothesis predicts that the source of the excess emission is due to dust, the small dust grains are sensitive to the ionisation state of the gas, and hence the spinning dust models have a dependancy on the abundance of the major gas ions. CII observations will enable us to investigate this dependancy, and combining these observations with the available mid- to far-IR observations will permit a complete analysis of the role of both the dust and gas in regions of anomalous emission. We request a total of 14.9 hrs of HIFI observing time.

  3. OT2_anoriega_2: SABER: Spectral Analysis of the Bowshock Emission in a Runaway

    NASA Astrophysics Data System (ADS)

    Noriega-Crespo, A.

    2011-09-01

    Bowshocks around runaway OB stars are some of the most spectacular objects in the mid/far infrared, covering in some cases as much as half a degree across the sky. The bowshocks are essentially enormous gas shells contained by ram pressure where the dust trapped in their interiors reprocesses the UV flux from the parent OB stars and re-radiates it in the infrared. The pressure balance between the stellar wind and the ISM, also implies a tight relationship between their physical properties, and therefore, bowshocks from runaway stars provide a powerful tool to probe the interstellar medium and/or the properties of the OB stellar wind. The formation of these shells requires very efficient cooling that is expected to take place through the emission of a wealth of atomic fine structure lines, like [OI] 63.2 or [NII] 205.2 micron. Because the diffuse nature of these shells it has been very difficult to confirm this expectation using spectroscopic observations. In this project we propose to use the PACS spectrometer to observe the zeta Oph bowshock in order to better understand and constrain the physical conditions of its gas shell, its dust properties, its turbulence, and in such a way that will allow to use zeta Oph as a template to make sense of the physical properties of the many more runaway bowshocks are continuously being discovered.

  4. Increased radiosensitivity of a subpopulation ot T-lymphocyte progenitors from patients with Fanconi's anemia

    SciTech Connect

    Knox, S.J.; Wilson, F.D.; Greenberg, B.R.; Shifrine, M.; Rosenblatt, L.S.; Reeves, J.D.; Misra, H.

    1981-06-01

    In vitro radiation survival of peripheral blood T lymphocytes was studied in 15 clinically normal adults and 4 patients with Fanconi's anemia. Tritiated thymidine incorporation in a whole blood lymphocyte stimulation test (LST) and a newly developed whole blood T-lymphocyte colony assay were used to measure lymphocyte blastogenesis and colony formation in response to phytohemagglutinin (PHA) or concanavalin-A (Con-A) stimulation. Lymphocyte colony formation was found to be consistently more sensitive than the LST for detection of low-level radiation effects using both normal cells and lymphocytes from Fanconi's anemia patients. Lymphocytes from patients with Fanconi's anemia were significantly more sensitive to in vitro x-irradiation than lymphocytes from clinically normal individuals as measured by their ability to divide when stimulated by PHA in the LST (patients, D37 . 198 R; normals, D37 . 309 R, p . 0.057) and colony formation assay (patients, D37 . 53 R; normals, D37 . 109 R, p . 0.016). No significant difference in the radiosensitivity of the Con-A response was observed between the two groups. The PHA-responsive T-lymphocyte subpopulation in Fanconi's anemia patients appears to be intrinsically defective. The nature of this defect, significance in the disease process, and relevancy of these findings to the establishment of radiation protection standards are discussed.

  5. Suivi d'une nouvelle UG du Lynx : MASTER OT J072948.66+593824.4

    NASA Astrophysics Data System (ADS)

    Morillon, E.

    2012-03-01

    Suite à la détection, par nos collègues russes, d'une nouvelle variable dans le Lynx mi- février 2012, et à la diffusion de l'alerte sur internet, j'ai pu pointer cette nouvelle cataclysmique de type UGSU, pendant 4h, avant qu'elle ne retourne à son état de repos. Après un rappel sur sa découverte, j'exposerai la façon dont je l'ai suivie 4 jours après la découverte et les quelques résultats que l'on peut en extraire.

  6. Swift UVOT observations of the SMC nova MASTER OT J010603.18-744715.8

    NASA Astrophysics Data System (ADS)

    Kuin, N. P. M.; Page, K. L.; Williams, S. C.; Darnley, M. J.; Shore, S. N.; Walter, F. M.

    2016-10-01

    Since its discovery at magnitude 10.9 on 2016-10-14.19341 UT (Shumkov et al, ATEL #9621) and spectroscopic confirmation (Williams and Darnley, ATEL #9628) pre-discovery data from the OGLE team (Mroz and Udalski, ATEL #9622, and MASTER (Lipunov et al., ATEL #9631) revealed the progenitor magnitude and pushed back the appearance of the nova to magnitude 8.5 on 2016, Oct 9.808.

  7. OT2_jsokolos_1: The Origin and Nature of the Emission Nebulae around Symbiotic Stars

    NASA Astrophysics Data System (ADS)

    Sokoloski, J.

    2011-09-01

    There is much controversy concerning the ionized nebula that produces the radio through FIR emission from symbiotic stars. The goal of the proposed Herschel observations is to test two popular models for this emission; whether it is produced by a wind from the red giant that is photoionized by Lyman continuum photons from the hot WD (STB) or it comes from plasma that is shock heated as the winds from the two stars collide by constraining the submm SED and measuring the free-free turnover frequency of the ionised component. These two models predict distinctly different shapes for the submm portion of the SED and different dependence of the turnover frequency on binary separation. Thus, submm photometry of a diverse sample of symbiotic stars with know binary parameters that only Herschel can perform is an ideal way to quantitatively test and discriminate between these models (as well as motivate new ones). In terms of astrophysical significance, determining the origin of the radio-through-FIR emission from symbiotic stars has implications for the nature and geometry of mass transfer in wide binaries, mass loss from accreting compact objects, the shaping of asymmetric nebulae around binary stars (including binary planetary nebulae), and the likelihood that symbiotic stars can explode as type Ia supernovae.

  8. OT2_bsmith_3: Spirals, Bridges, and Tails: The Herschel View of Dust in Interacting Galaxies

    NASA Astrophysics Data System (ADS)

    Smith, B.

    2011-09-01

    The tidal features produced by gravitational interactions between galaxies may contribute significantly to the enrichment of the intergalactic medium in dust and heavy elements. However, at the present time little is known about the dust content and properties of tidal structures. To address this lack, we propose to use the PACS and SPIRE instruments on Herschel to image a sample of nine nearby interacting galaxies in six far-infrared/submm broadband filters. We will map the dust column density and temperature in the main bodies and tidal features of these galaxies, and compare the far-infrared/submm properties of these features with those of normal spirals and dwarf galaxies. We will compare the Herschel maps with already acquired GALEX UV, Spitzer IR, and ground-based optical data, and with population synthesis and radiative transfer codes, to investigate dust heating mechanisms and extinction in these galaxies. We will compare with available radio maps to investigate dust/gas ratios and star formation triggering mechanisms, and compare with numerical simulations of the interactions. Our sample includes the closest and best-studied examples of tidal dwarf galaxies and accretion-driven star formation. These will provide a good testbed for interpreting high redshift systems.

  9. OT1_pappleto_1: Charting the Cooling Pathways in High-Speed Extragalactic Shocks

    NASA Astrophysics Data System (ADS)

    Appleton, P.

    2010-07-01

    Following in the footsteps of ISO, recent observations with Spitzer have revealed a population of galaxy systems which emit a huge amount of luminosity in their pure rotational mid-IR molecular hydrogen lines, in some cases reaching 10-30 percent of the bolometric luminosity. These large line-luminosities are believed to be powered by galactic-scale shocks, which efficiently transfer kinetic energy to smaller dense clouds in the turbulent post-shock medium. However, nothing at all is known about the other important cooling channels for the shocked gas, such as [OI], H2O, OH, and CO, some of which can rival H2 as a coolant. We propose deep PACS and SPIRE spectroscopy of the Giant Shock in Stephan's Quintet and the Taffy Galaxy bridge to quantify the most important cooling channels and determine the physical state of the gas being shocked. The results have implications for understanding the importance of molecular cooling at higher redshift where turbulence and shock-heating may play a role in galaxy formation.

  10. 78 FR 54401 - Removal of Transferred OTS Regulations Regarding Post-Employment Activities of Senior Examiners

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-04

    ... of the 550 17th Street Building (located on F Street) on business days between 7 a.m. and 5 p.m... the Federal Register on July 6, 2011.\\2\\ \\2\\ 76 FR 39247 (July 6, 2011). Although section 312(b)(2)(B... rescinding them, as appropriate. \\3\\ 76 FR 47652 (August 5, 2011). One of the regulations transferred to...

  11. Neutron Reflection Study of Bovine β-Casein Adsorbed on OTS Self- Assembled Monolayers

    NASA Astrophysics Data System (ADS)

    Fragneto, Giovanna; Thomas, Robert K.; Rennie, Adrian R.; Penfold, Jeffrey

    1995-02-01

    Specular neutron reflection has been used to determine the structure and composition of bovine β-casein adsorbed on a solid surface from an aqueous phosphate-buffered solution at pH 7. The protein was adsorbed on a hydrophobic monolayer self-assembled from deuterated octadecyltrichlorosilane solution on a silicon (111) surface. A two-layer structure formed consisting of one dense layer of thickness 23 ± 1 angstroms and a surface coverage of 1.9 milligrams per square meter adjacent to the surface and an external layer protruding into the solution of thickness 35 ± 1 angstroms and 12 percent protein volume fraction. The structure of the (β-casein) layer is explained in terms of the charge distribution in the protein.

  12. Managing Occupational Therapy in Rural Education: O.T. Student Perceptions of Adaptive Classroom Behaviors.

    ERIC Educational Resources Information Center

    Dunn, Winnie; Hughes, Paula

    This report describes the Managing Occupational Therapy in Rural Education (MORE) a project that is designing a preservice training curriculum for occupational therapy students preparing to work in rural settings. Therapists need to have an understanding of rural school systems, educational aims, philosophy, and the structure of the system itself…

  13. OT1_nlu_1: Herschel Spectroscopic Survey of Warm Molecular Gas in Local Luminous Infrared Galaxies

    NASA Astrophysics Data System (ADS)

    Lu, N.

    2010-07-01

    We propose to survey CO spectral line energy distribution (SLED), from J=4-3 up to J=13-12, on 93 local luminous infrared galaxies (LIRGs; L_{IR} > 1.0E11 L_{sun}) with Herschel SPIRE FTS spectrometer. These galaxies, plus 32 additional LIRGs that will have similar data from existing Herschel programs (mainly the HerCULES project), form a flux-limited subset of the Great Observatories All-Sky LIRGs Survey (GOALS) sample. Our proposal is built on the legacy of GOALS and extends beyond the existing Herschel HerCULES program, which emphasizes more on ULIRGs, to a much needed sample coverage of the more numerous and diverse population of less luminous LIRGs. The data from the proposed observations will not only provide much needed local LIRG templates for future ALMA studies of high-redshift counterparts, but also lend us a powerful diagnostic tool to probe the warm and dense molecular gas that are more closely related to the starburst or AGN activity in the nuclei of LIRGs. The data from this proposal will provide important statistical clues to the interplay between the cold and warm molecular gas, IR luminosity, star formation rate and efficiency, and the diverse properties of LIRGs. Specifically, using the homogeneous CO SLED data from this proposal, together with ground-base, low-order CO line data (mainly J=1-0) and other data that have been compiled for the GOALS sample, we will address the following questions: (1) What is the dominant nuclear power source in individual sample galaxy: starburst or AGN? (2) What are the typical physical properties of warm molecular gas in the nuclei of LIRGs? (3) How do the nuclear warm gas components correlate to the cold gas component, star formation rate and efficiency, dust temperature, etc? and (4) How does molecular gas excitation change along a merger sequence?

  14. OT2_cceccare_4: Searching for the onset of energetic particle irradiation in Class 0 protostars

    NASA Astrophysics Data System (ADS)

    Ceccarelli, C.

    2011-09-01

    Several evidences tell us that the first stages of low mass star formation are very violent, characterized by, among other phenomena, an intense irradiation of energetic (MeV) particles. The goal of this proposal is to search for signs of MeV particle irradiation in a sample of low to intermediate mass Class 0 protostars. At this end, we propose to observe a selected list of high J HCO+ and N2H+ lines in a selected sample of sources. Based on the observations obtained within the KP CHESS, we estimate a total observing time of 20.5 hours.

  15. Experimental Validation of OT Solutions to the Comprehension-Production Dilemma

    ERIC Educational Resources Information Center

    Gierut, Judith A.

    2006-01-01

    The relationship between perception and production remains an unresolved issue within the study of phonological acquisition. Recent developments in optimality theory offer potentially new solutions to this long-standing problem; but thus far, the proposals that have been advanced are in the absence of actual perception-production data from a given…

  16. OT1_sveilleu_1: Cold Gas and Dust in the Filamentary Complexes of Cluster Cooling Flows

    NASA Astrophysics Data System (ADS)

    Veilleux, S.

    2010-07-01

    We propose to obtain deep PACS + SPIRE far-IR photometry and 2D PACS [C II] 158 um spectroscopy of 7 cooling-flow clusters with extended Halpha filaments to constrain the properties of the dust and cooling gas in these systems. Our targets are part of a large sample of clusters that were imaged at Halpha using the Maryland Magellan Tunable Filter (MMTF) as part of a comprehensive multiwavelength survey. The superb sensitivity and resolution of the MMTF data have uncovered, often for the very first time, spectacular filaments of warm ionized gas extending as far as 50 kpc from the cluster core. The origin and heating source of these filaments remains a mystery. Our MMTF images have served as a guide to extract on-filament X-ray and optical spectra for a large sample of clusters, leading to the discovery that the X-ray ICM is cooling at a highly accelerated rate in the vicinity of these filaments. This direct link between the warm and hot phases may be the first direct evidence for the purported cooling flow. Given the high FIR fluxes detected in cluster cores, it is possible that dust grain cooling plays an important role in the cooling flow process. However, it remains unclear whether the observed dust is associated with the warm filaments or limited to the center of the brightest cluster galaxy (BCG). Current key programs cannot address this question given their lack of information on the Halpha filaments. Our proposed Herschel observations of the 7 cooling-flows clusters from our sample with the most extended Halpha filaments will directly address this question and help us determine: a) the role of dust cooling in the cooling-flow process, b) whether the observed dust is associated with the BCG or the warm filaments, and c) whether gas is cooling below 10^4 K, the temperature probed by Halpha. Providing answers to these questions will improve our understanding of the cooling processes in galaxy clusters and constrain the role of heating processes, such as AGN feedback, in preventing such cooling.

  17. OT2_amoor_4: A census of debris disks in nearby young moving groups with Herschel.

    NASA Astrophysics Data System (ADS)

    Moór, A.

    2011-09-01

    Nearly all young stars harbour circumstellar disks, that serve as the reservoir for mass accretion onto the star, and later become the birthplace of planetary systems. After the disappearance of the gas component from the disk a dusty debris disk is formed that is believed to mark the location of the planetesimal belt as well. For outlining the evolution of such debris disks traditionally open clusters and field stars were studied, however we argue that the recently discovered young moving groups are more suitable objects for such analyses, due to their proximity and good coverage of the first 50 Myr period of the planetary system evolution. In this proposal we request 70/160 um Herschel/PACS photometric observations for so-far unobserved moving group members. These observations will provide a complete coverage of all known members within 80 pc of five nearby young moving groups (beta Pic Moving Group, Tucana-Horologium, Carina, Columba, and Argus), in the A to K spectral range. Based on the new observations we will identify new debris disks, characterize the disk population within individual moving groups, and study disk evolution by comparing the groups of different ages. The results will be used to verify predictions of the self-stirring model of the evolution of planetesimal disks. We will also compare the properties of debris disks in groups of the same age, looking for additional 'environmental' parameters that affect disk structure over a whole moving group. Our study will be a significant contribution to the census of debris disks in young moving groups, increasing the number of observed sources by a factor of 1.5. Since Spitzer could perform only a limited census and the so-far approved Herschel programs added very few additional moving group obervations, our programme is expected to have a high legacy value.

  18. The Transition from Spacecraft Development Ot Flight Operation: Human Factor Considerations

    NASA Technical Reports Server (NTRS)

    Basilio, Ralph R.

    2000-01-01

    In the field of aeronautics and astronautics, a paradigm shift has been witnessed by those in academia, research and development, and private industry. Long development life cycles and the budgets to support such programs and projects has given way to aggressive task schedules and leaner resources to draw from all the while challenging assigned individuals to create and produce improved products of processes. however, this "faster, better, cheaper" concept cannot merely be applied to the design, development, and test of complex systems such as earth-orbiting of interplanetary robotic spacecraft. Full advantage is not possible without due consideration and application to mission operations planning and flight operations, Equally as important as the flight system, the mission operations system consisting of qualified personnel, ground hardware and software tools, and verified and validated operational processes, should also be regarded as a complex system requiring personnel to draw upon formal education, training, related experiences, and heuristic reasoning in engineering an effective and efficient system. Unquestionably, qualified personnel are the most important elements of a mission operations system. This paper examines the experiences of the Deep Space I Project, the first in a series of new technology in-flight validation missions sponsored by the United States National Aeronautics and Space Administration (NASA), specifically, in developing a subsystems analysis and technology validation team comprised of former spacecraft development personnel. Human factor considerations are investigated from initial concept/vision formulation; through operational process development; personnel test and training; to initial uplink product development and test support. Emphasis has been placed on challenges and applied or recommended solutions, so as to provide opportunities for future programs and projects to address and disposition potential issues and concerns as early as possible to reap the benefits associated with learning from other's past experiences.

  19. Visible-light driven water splitting over BiFeO₃ photoanodes grown via the LPCVD reaction of [Bi(OtBu)₃] and [Fe(OtBu)₃]₂ and enhanced with a surface nickel oxygen evolution catalyst.

    PubMed

    Moniz, Savio J A; Blackman, Christopher S; Southern, Paul; Weaver, Paul M; Tang, Junwang; Carmalt, Claire J

    2015-10-21

    Phase-pure BiFeO3 films were grown directly via dual-source low-pressure CVD (LPCVD) from the ligand-matched precursors [Bi(O(t)Bu)3] and [Fe(O(t)Bu)3]2, without the requirement for oxidising gas or post deposition annealing. Photocatalytic testing for water oxidation revealed extremely high activity for PEC water splitting and photocatalytic water oxidation under visible light irradiation (λ > 420 nm) with a benchmark IPCE for BiFeO3 of 23% at 400 nm. The high activity is ascribed to the ultrafine morphology achieved via the LPCVD process. The performance was enhanced by over four times when the BiFeO3 photoanode is coupled to a Ni-B surface OEC.

  20. [Diffuse xanthogranulomatous pyelonephritis: clinical, anatomopathologic, and therapeutic features. Report ot 9 cases and review of the literature].

    PubMed

    Dahami, Z; Dakir, M; Aboutaieb, R; Sarf, I; Elattar, H; Azzouzi, S; Bennani, S; Elmrini, M; Benjelloun, S

    2001-11-01

    Diffuse xanthogranulomatous pyelonephritis is a rare chronic pyelonephritis. This study permitted us to notice the various histological, clinical, radiological, and the different means diagnoses of this affection. Retrospectively series of nine patients is reported. The median age was 43 years. The commonest clinical presentation was urinary tract infection, and lumbar pain. Urine culture identified a bacteria in seven cases (77%). Intravenous urography recovers silent kidney (five cases), hydronephrosis (two cases), chronic pyelonephritis (one case) and renal stones in six cases (67%). Treatment consisted of nephrectomy. No specific biological or radiological signs of this affection can be identifying this lesion in preoperative. The diagnosis was revealed by the histological examination of the removed kidney. The outcome is favorable after a follow-up of six months to four years. The clinical features of the pyelonephritis xanthogranulomatous is not specific. Magnetic resonance imaging can give a specificity to this affection. Only histological examination is able to established the definitive diagnosis.

  1. "[N]ot subject to our sense” : Margaret Cavendish's fusion of Renaissance science, magic and fairy lore.

    PubMed

    Walters, Lisa

    2010-01-01

    This article explores Margaret Cavendish's depictions of alchemy, witchcraft and fairy lore in her scientific treatise Philosophical Letters and in fictional texts from Natures Pictures and Poems and Fancies. Though Cavendish was a dedicated materialist, she appropriates theories of magic from early modern science and folklore into her materialist epistemology. As Cavendish draws upon a fusion of early modern conceptions of magic, she creates a radical theory of matter which not only challenges patriarchy and binary oppositions, but also explores the plurality and mystery that can exist within an infinitely complex material world. PMID:21275192

  2. Learning in a Simulation-OT in Heart Surgery and the Challenges of the Scientification of Work

    ERIC Educational Resources Information Center

    Langemeyer, Ines

    2014-01-01

    Enhancing competency and collaboration has become a salient topic of the professional debate on medical safety issues. The advantages of simulation-based training scenarios for team communication, routines and critical work procedures especially in operation theatres have been vigorously discussed. However, the literature on simulation-based…

  3. OT1_gstacey_3: A PACS Redshift 1-2 Oxygen Survey: Leveraging the ZEUS [CII] Detections

    NASA Astrophysics Data System (ADS)

    Stacey, G.

    2010-07-01

    We propose to use PACS spectroscopy to observe the [OI] 63 um, [OIII] 88 and 52 um, and [OIV] 26 um fine-stucture lines, and PACS/SPIRE photometry to measure the far-IR continuum from 12 IR-bright galaxies in the z = 1 to 2 redshift range. These are galaxies from which we have detected emission in the 158 um [CII] line using our grating spectrometer, ZEUS, on the CSO. We have found that the most luminous starburst-dominated systems in this epoch are characterized by kpc-scale moderate intensity star formation, while the AGN-dominated systems host similarly extended, but much more intense starbursts. The proposed oxygen survey addresses two key questions stimulated by our [CII] results: (1) To what extent are luminous star-forming galaxies at z = 1 to 2 simply scaled-up versions of local starbursts? (2) Why are the starbursts in AGN-dominated systems so much more intense? The oxygen sequence combined with our [CII] detections will have powerful diagnostic capabilities, yielding the strength and hardness of the ambient UV radiation fields, and the density, pressure, and mass of the ionized and neutral atomic components. This data will allow us to characterize the size and age of the starburst, and the importance of the central engine. The ultimate goal is to understand what drives the apparently galaxy-wide starbursts in both star-formation-dominated and AGN-dominated systems, and help identify the connection between starbursts and AGN in the early Universe. This survey is important and unique. We cover the redshift interval from 1 to 2 near the peak of the star formation per unit co-moving volume in the Universe, and it is within this redshift interval that the ZEUS and PACS sensitivities are well matched to enable the detection of the [CII] line together with the oxygen sequence in a wide variety of systems.

  4. 75 FR 181 - Application for Conversion From: (a) OTS-Regulated, State-Chartered Savings Association to...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-04

    ... Association to Federal Savings Association; (b) National Bank, Commercial Bank, State Savings Bank, or Credit... continuing information collections, as required by the Paperwork Reduction Act of 1995, 44 U.S.C. 3507. The... collection requirement described below to the Office of Management and Budget (OMB) for review, as...

  5. Crystal structure of product-bound complex of UDP-N-acetyl-D-mannosamine dehydrogenase from Pyrococcus horikoshii OT3

    SciTech Connect

    Pampa, K.J.; Lokanath, N.K.; Girish, T.U.; Kunishima, N.; Rai, V.R.

    2014-10-24

    Highlights: • Determined the structure of UDP-D-ManNAcADH to a resolution of 1.55 Å. • First complex structure of PhUDP-D-ManNAcADH with UDP-D-ManMAcA. • The monomeric structure consists of three distinct domains. • Cys258 acting as catalytic nucleophilic and Lys204 acts as acid/base catalyst. • Oligomeric state plays an important role for the catalytic function. - Abstract: UDP-N-acetyl-D-mannosamine dehydrogenase (UDP-D-ManNAcDH) belongs to UDP-glucose/GDP-mannose dehydrogenase family and catalyzes Uridine-diphospho-N-acetyl-D-mannosamine (UDP-D-ManNAc) to Uridine-diphospho-N-acetyl-D-mannosaminuronic acid (UDP-D-ManNAcA) through twofold oxidation of NAD{sup +}. In order to reveal the structural features of the Pyrococcus horikoshii UDP-D-ManNAcADH, we have determined the crystal structure of the product-bound enzyme by X-ray diffraction to resolution of 1.55 Å. The protomer folds into three distinct domains; nucleotide binding domain (NBD), substrate binding domain (SBD) and oligomerization domain (OD, involved in the dimerization). The clear electron density of the UDP-D-ManNAcA is observed and the residues binding are identified for the first time. Crystal structures reveal a tight dimeric polymer chains with product-bound in all the structures. The catalytic residues Cys258 and Lys204 are conserved. The Cys258 acts as catalytic nucleophile and Lys204 as acid/base catalyst. The product is directly interacts with residues Arg211, Thr249, Arg244, Gly255, Arg289, Lys319 and Arg398. In addition, the structural parameters responsible for thermostability and oligomerization of the three dimensional structure are analyzed.

  6. OT1_mhaas_2: The Herschel Legacy of powerful 3C radio galaxies and quasars at z<1

    NASA Astrophysics Data System (ADS)

    Haas, M.

    2010-07-01

    We propose Herschel observations of a complete sample of 3C radio-galaxies and quasars at redshift z<1. For all sources Spitzer mid-IR spectra are available. The aim is to quantify the orientation-dependence of AGN radiation (AGN unification), to investigate the interplay between accretion onto the central black-hole and star-formation in the hosts, and to understand the evolution of the black-hole/stellar-bulge relation. The low-frequency radio-selection provides us with powerful and massive active galaxies free from any orientation/obscuration bias, a requirement for testing AGN unification. The properties of the 3C sources are well known throughout the electromagnetic spectrum, except in the far-IR/sub-mm, where most of them were hitherto outside the capability of space missions to reach reliable measurements. We propose PACS 70-160 micron photometry of 72 sources supplemented by SPIRE 250-500 micron photometry of 11 sufficiently bright sources, in order to measure their detailed spectral energy distributions between available Spitzer and SCUBA/MAMBO data. Depending on redshift of the sources and predicted flux, the filter choice is optimised to provide best rest-frame FIR coverage. The rest-frame FIR emission serves as an isotropic calorimeter and the MIR/FIR luminosity ratio is determined by the relative strength of the AGN and star-forming contributions combined with dust obscuration. These observations will return crucial new information on the energy processes in powerful AGN and their hosts at z<1, providing an essential anchor for studies of galaxy and AGN evolution, in particular for a consistent comparison with Herschel observations of several (radio-loud) AGN samples at cosmic epochs z>1.

  7. The first N-terminal unprotected (Gly-Aib)n peptide: H-Gly-Aib-Gly-Aib-OtBu.

    PubMed

    Gessmann, Renate; Brückner, Hans; Petratos, Kyriacos

    2015-12-01

    Glycine (Gly) is incorporated in roughly half of all known peptaibiotic (nonribosomally biosynthesized antibiotic peptides of fungal origin) sequences and is the residue with the greatest conformational flexibility. The conformational space of Aib (α-aminoisobutyric acid) is severely restricted by the second methyl group attached to the Cα atom. Most of the crystal structures containing Aib are N-terminal protected. Deprotection of the N- or C-terminus of peptides may alter the hydrogen-bonding scheme and/or the structure and may facilitate crystallization. The structure reported here for glycyl-α-aminoisobutyrylglycyl-α-aminoisobutyric acid tert-butyl ester, C16H30N4O5, describes the first N-terminal-unprotected (Gly-Aib)n peptide. The achiral peptide could form an intramolecular hydrogen bond between the C=O group of Gly1 and the N-H group of Aib4. This hydrogen bond is found in all tetrapeptides and N-terminal-protected tripeptides containing Aib, apart from one exception. In the present work, this hydrogen bond is not observed (N...O = 5.88 Å). Instead, every molecule is hydrogen bonded to six other symmetry-related molecules with a total of eight hydrogen bonds per molecule. The backbone conformation starts in the right-handed helical region (and the left-handed helical region for the inverted molecule) and reverses the screw sense in the last two residues. PMID:26632841

  8. Multilocus phylogenetic analysis of true morels (Morchella) reveals high levels of endemics in Turkey relative ot other regions of Europe

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The present study was conducted to better understand how the phylogenetic diversity of true morels (Morchella) in Turkey compares with species found in other regions of the world. The current research builds on our recently published survey of 10 Turkish provinces and another of the world in which D...

  9. OT1_gorton01_1: Variability in Ice Giant Stratospheres: Implications for Radiative, Chemical and Dynamical Processes

    NASA Astrophysics Data System (ADS)

    Orton, G.

    2010-07-01

    We will assess the rotational variability in the stratospheres of the ice giants, Uranus and Neptune, to understand the dynamical and chemical variability of the atmospheric structure of both planets as a function of longitude. This effort follows up observations by the Spitzer IRS that shows consistent evidence for rotational variability of stratospheric hydrocarbons in Uranus and intermediate-term variability in Neptune's emissions, neither of whose origins are not well understood. Herschel provides an opportunity to follow up these observations with its unparalleled sensitivity. Over the 17-hour periods characterizing the equatorial rotation periods of both planets a series of eight PACS dedicated line scans will be made of strategic lines of HD, methane and water vapor. An efficient scheme takes advantage of the simultaneous availability of Uranus and Neptune in Herschel's visibility window. These will assess the variability of hydrocarbons vs temperatures in both atmospheres to an unprecedented accuracy. The results will be analyzed by a team consisting of many members of the Key Project on ``Water and Related Chemistry in the Solar System'' who will apply their expertise with the data and its analysis, as well as researchers who discovered the Spitzer variability and ground-based inhomogeneity. The data will be examined in the context of models by team members who are experts in radiative transfer, photochemistry, and dynamical modeling of circulation and zonal thermal wave structure. By refining quantitative models for interactions between radiative, dynamical and chemical processes in these two cold but radiatively and dynamically diverse planets, a baseline will be created that will be useful in the interpretation of variability in the spectra of giant exoplanets. This work will also be programmatically useful in the evaluation of the variability of radiation from Uranus and Neptune, both which are key members of the Herschel flux calibration system.

  10. ICT & OTs: a model of information and communications technology acceptance and utilisation by occupational therapists (part 2).

    PubMed

    Schaper, Louise; Pervan, Graham

    2007-01-01

    The research reported in this paper describes the development, empirical validation and analysis of a model of technology acceptance by Australian occupational therapists. The study described involved the collection of quantitative data through a national survey. The theoretical significance of this work is that it uses a thoroughly constructed research model, with one of the largest sample sizes ever tested (n=1605), to extend technology acceptance research into the health sector. Results provide strong support for the model. This work reveals the complexity of the constructs and relationships that influence technology acceptance and highlights the need to include sociotechnical and system issues in studies of technology acceptance in healthcare to improve information system implementation success in this arena. The results of this study have practical and theoretical implications for health informaticians and researchers in the field of health informatics and information systems, tertiary educators, Commonwealth and State Governments and the allied health professions.

  11. OT1_rrubin_1: Herschel's Opportunity to Solve the Nebular Abundance Problem While Creating a Legacy Planetary Nebulae Dataset

    NASA Astrophysics Data System (ADS)

    Rubin, R.

    2010-07-01

    Abundance surveys of a large sample of Galactic planetary nebulae (PNe) have led to the discovery of a group of super-metal-rich nebulae whose spectra show prominent optical recombination lines (ORLs) from C, N, O, and Ne ions. The heavy element abundances derived from ORLs for several PNe are a factor >10 higher than those derived from the traditional method based on collisionally excited lines (CELs). This ratio is called the abundance discrepancy factor (adf). A promising proposition to explain the nebular abundance problem posits that these nebulae contain (at least) two distinct regions - one of "normal" electron temperature, Te (~10000 K) and chemical composition (~solar) and another of very low Te (< 1000) that is H-deficient, thus having high metal abundances relative to H. The latter component emits strong heavy element ORLs and IR fine-structure (FS) CELs but essentially no optical/UV CELs. Efforts to directly detect these inclusions in PNe have been unsuccessful to date. However, there is mounting circumstantial evidence for their existence, such as presented in our recent paper that modeled the high-adf PN NGC 6153 using a 3-D photoionization code. The models that included the low Te, H-deficient knots fit most observations far better than did those models without the clumps. With the launch of Herschel, there is finally the capability to perform a test we've been dreaming of. Measurements have shown that the adf varies with position in a PN and is highest close to the central star. The very low Te inclusions must be cooled via FS IR lines. We propose to use Herschel to map the FS IR lines in 5 bright PNe on the largest adf list, to find if these lines peak where the adf peaks. These spectra will also provide a feast for our other team expertise/interests: a legacy dataset of molecular lines to explore PDRs, how the central star interacts with the AGB ejecta and shapes the PN, how the shocks are produced, what comprises the chemistry of the molecular ejecta, and how do PNe evolve.

  12. "[N]ot subject to our sense” : Margaret Cavendish's fusion of Renaissance science, magic and fairy lore.

    PubMed

    Walters, Lisa

    2010-01-01

    This article explores Margaret Cavendish's depictions of alchemy, witchcraft and fairy lore in her scientific treatise Philosophical Letters and in fictional texts from Natures Pictures and Poems and Fancies. Though Cavendish was a dedicated materialist, she appropriates theories of magic from early modern science and folklore into her materialist epistemology. As Cavendish draws upon a fusion of early modern conceptions of magic, she creates a radical theory of matter which not only challenges patriarchy and binary oppositions, but also explores the plurality and mystery that can exist within an infinitely complex material world.

  13. OT1_dpadgett_1: PACS Photometry of Nearby Warm Debris Disk Systems from the WISE All-Sky Survey

    NASA Astrophysics Data System (ADS)

    Padgett, D.

    2010-07-01

    Debris disks trace the collisional breakdown of asteroid and comet parent bodies orbiting nearby main sequence stars. They are detectable in ~16% of FGK stars, nearly twice as often in A stars, and are almost unknown around M stars. The debris disks of sunlike stars are typically cold analogs of our Kuiper belt with emission peaking near 70 microns wavelength. However, a relatively small number of warm disks are known with emission at 24 microns. These systems are especially interesting because they trace dust in the region likely to host terrestrial planets, where the dust has a short dynamical lifetimes. They also tend to be young systems aged < 1 Gyr. This knowledge of warm debris disks - extrasolar analogs to our solar system's Zodiacal cloud - is based on the 25 year old IRAS survey and observations of selected targets with ISO and Spitzer. The Wide-Field Infrared Survey Explorer (WISE) has just completed new, sensitive all-sky mapping in the 3.3, 4.6, 12, and 22 micron bands. Association of the WISE sources to Hipparcos and Tycho stars has led to the identification of 99 nearby main sequence stars with robustly detected warm 22 micron excesses not previously known. To determine whether these systems represent outbursts of asteroidal dust production (such as in the HD 69830 system), or simply the Wien side of emission from a cold outer dust belt, photometry at longer wavelengths is needed. We propose Herschel/PACS 70 and 160 micron photometry of this unbiased sample. These data will allow us to fully characterize the dust temperature and infrared luminosity of these systems, allowing them to be understood in the context of other debris disks and disk evolution theory. The sample includes field M stars as close as 12 pc, the first objects of this class seen to have warm dust emission. The results will strongly constrain our picture of the collisional history of inner planetary systems.

  14. OT1_sbontemp_1: Water emission from outflows and hot cores in the Cygnus X proto-stars

    NASA Astrophysics Data System (ADS)

    Bontemps, S.

    2010-07-01

    The impressive first results from the WISH GT key program by van Dishoeck et al. indicate that water emission is bright towards the embedded proto-stars of all masses. These emissions are tracing outflows and warm inner regions of the collapsing envelopes (radiatively heated hot cores) which are unique probes of the cooling of these regions and of the kinematics of the dense warm gas. But WISH is limited by the reduced number of targets, and by the unavoidable biases introduced by the stringent selection of sources. The intermediate to high mass range is critical to challenge protostellar evolution models, and we argue that water emission from a complete sample of proto-stars in this mass range will be an important piece of knowledge for outflows to trace indirectly accretion and for hot cores to follow their time of appearance. Only Cygnus X is nearby and rich enough to provide a large sample of such proto-stars. We propose here to dramatically change the level of significance of WISH results by observing as many as 92 proto-stars covering the (final stellar) mass range of 3 to 20 Msun in the single complex of Cygnus X.

  15. The first N-terminal unprotected (Gly-Aib)n peptide: H-Gly-Aib-Gly-Aib-OtBu.

    PubMed

    Gessmann, Renate; Brückner, Hans; Petratos, Kyriacos

    2015-12-01

    Glycine (Gly) is incorporated in roughly half of all known peptaibiotic (nonribosomally biosynthesized antibiotic peptides of fungal origin) sequences and is the residue with the greatest conformational flexibility. The conformational space of Aib (α-aminoisobutyric acid) is severely restricted by the second methyl group attached to the Cα atom. Most of the crystal structures containing Aib are N-terminal protected. Deprotection of the N- or C-terminus of peptides may alter the hydrogen-bonding scheme and/or the structure and may facilitate crystallization. The structure reported here for glycyl-α-aminoisobutyrylglycyl-α-aminoisobutyric acid tert-butyl ester, C16H30N4O5, describes the first N-terminal-unprotected (Gly-Aib)n peptide. The achiral peptide could form an intramolecular hydrogen bond between the C=O group of Gly1 and the N-H group of Aib4. This hydrogen bond is found in all tetrapeptides and N-terminal-protected tripeptides containing Aib, apart from one exception. In the present work, this hydrogen bond is not observed (N...O = 5.88 Å). Instead, every molecule is hydrogen bonded to six other symmetry-related molecules with a total of eight hydrogen bonds per molecule. The backbone conformation starts in the right-handed helical region (and the left-handed helical region for the inverted molecule) and reverses the screw sense in the last two residues.

  16. OT2_pghavami_1: Herschel imaging of SNR G292.0+1.8: Cas A's older cousin?

    NASA Astrophysics Data System (ADS)

    Ghavamian, P.

    2011-09-01

    It is well-known that supernovae play a key role in creating and distributing elements throughout the universe, but less well-known is their contribution to the overall budget of dust in the ISM. Theoretical models suggest that core-collapse supernovae should produce large quantities of dust, but observational evidence for this is still debated. Even if significant quantities of dust are produced, does it survive the passage of the reverse shock to enter the ISM? Does the forward shock destroy all dust that it encounters? Near and mid-IR observations with Spitzer and AKARI have begun to answer these questions, but the long wavelength cameras of Herschel are necessary for a complete picture. We propose detailed observations of G292.0+1.8, a large Galactic supernova remnant (SNR) that has been called the "older cousin" of Cassiopeia A. One of the few known oxygen-rich remnants, G292 is a 3000 year old textbook example of a core-collapse SNR expanding into its own circumstellar medium (CSM), the wind of a red giant. It is one of the most well-studied SNRs at all wavelengths, from radio to X-rays. At 8' in diameter, it is large enough for the emission from the forward-shock CSM to be well-separated from that of the reverse-shocked ejecta, yet it is still small enough to be fully covered by Herschel in a reasonably small amount of time. We will obtain PACS and SPIRE imaging of the entire remnant. PACS observations will be sensitive to forward-shocked material, while SPIRE data will tell us whether large amounts of ejecta dust are present in the remnant. We will use the far-IR data in conjunction with X-ray and optical data to obtain a complete picture of the dynamical evolution of the remnant, and advance our understanding of the nature of dust in the universe.

  17. Crystallization and preliminary X-ray crystallographic analysis of a conserved domain in plants and prokaryotes from Pyrococcus horikoshii OT3

    SciTech Connect

    Lin, Linyen; Nakano, Hiroaki; Uchiyama, Susumu; Fujimoto, Satoru; Matsunaga, Sachihiro; Nakamura, Shota; Kobayashi, Yuji; Ohkubo, Tadayasu; Fukui, Kiichi

    2005-04-01

    A plant- and prokaryote-conserved domain (PPC) has been crystallized. The crystal diffracted to 1.7 Å resolution and belonged to space group P6{sub 3}22. A plant- and prokaryote-conserved domain (PPC) has previously been found in AT-hook motif nuclear localized protein 1 (AHL1) localized in the nuclear matrix of Arabidopsis thaliana (AtAHL1). AtAHL1 has a DNA-binding function. Mutation analyses of AtAHL1 has previously revealed that the hydrophobic region of the PPC domain is essential for its nuclear localization. In this study, the PPC of the hyperthermophilic archaebacterium Pyrococcus horikoshii (PhPPC) was crystallized using the hanging-drop vapour-diffusion method. The crystals belonged to the hexagonal space group P6{sub 3}22, with unit-cell parameters a = b = 53.69, c = 159.2 Å. Data were obtained at 100 K, with diffraction being observed to a resolution of 1.7 Å. A complete data set from crystals of the SeMet-substituted protein was also obtained.

  18. Subject and authorship of records related to the Organization for Tropical Studies (OTS) in BINABITROP, a comprehensive database about Costa Rican biology.

    PubMed

    Monge-Nájera, Julián; Nielsen-Muñoz, Vanessa; Azofeifa-Mora, Ana Beatriz

    2013-06-01

    BINABITROP is a bibliographical database of more than 38000 records about the ecosystems and organisms of Costa Rica. In contrast with commercial databases, such as Web of Knowledge and Scopus, which exclude most of the scientific journals published in tropical countries, BINABITROP is a comprehensive record of knowledge on the tropical ecosystems and organisms of Costa Rica. We analyzed its contents in three sites (La Selva, Palo Verde and Las Cruces) and recorded scientific field, taxonomic group and authorship. We found that most records dealt with ecology and systematics, and that most authors published only one article in the study period (1963-2011). Most research was published in four journals: Biotropica, Revista de Biología Tropical/ International Journal of Tropical Biology and Conservation, Zootaxa and Brenesia. This may be the first study of a such a comprehensive database for any case of tropical biology literature.

  19. OT1_csanch01_1: Molecular complexity of the O-rich pre-Planetary Nebula OH231.8

    NASA Astrophysics Data System (ADS)

    Sánchez Contreras, C.

    2010-07-01

    Envelopes around evolved stars are extraordinarily efficient factories of complex molecules and dust particles that will eventually enrich the ISM. To date, most chemistry studies have focused on C-rich envelopes, expected to show the largest molecular variety and abundances. Recent observations suggest that O-rich shells may be as chemically diverse as their Carbon counterparts, however, no serious attempt has been made yet to perform a full frequency scan of such objects. We propose to carry out a line survey with HIFI of the well known, O-rich pre-PN OH 231.8, which displays fast (>400 km/s), bipolar outflows and a molecular richness unparalleled amongst O-rich AGB and post-AGB stars. Results from our exploratory mm line survey with the IRAM 30 m antenna of this object has led to the detection of hundreds of lines from different species that clearly point to non-equilibrium/shock-induced molecule formation processes in the fast flow. The HIFI could be potentially effective to reveal the dense and warm regions near the centre, which is strongly affected by shocks. At its completion, this project will provide the sorely needed molecular inventory and abundance measurements in O-rich CSEs, which are required to obtain a better understanding of the intricate, non-equilibrium molecule formation processes in O-rich environments. We will use our radiative transfer and chemistry models to reproduce the observed spectra and empirically derived abundances, respectively, and constrain the nebular physical and chemical conditions in the envelope of OH231.

  20. [Characteristics of floor litter and soil arthropod community in different types ot subtropical forest in Ailao Mountain of Yunnan, Southwest China].

    PubMed

    Yang, Zhao; Yang, Xiao-Dong

    2011-11-01

    By using line transect method, an investigation was conducted on the floor litter and soil arthropod community in a mid mountain wet evergreen broad-leaved forest, a mossy dwarf forest, and a Populus bonatii forest in Ailao Mountain of Yunnan in April (dry and hot season), June (rainy season), and December (dry and cold season), 2005. In both dry and rainy seasons, the existing floor litter mass, C storage, and C/N ratio in the three forests all increased in the order of mossy dwarf forest > P. bonatii forest > evergreen broad-leaved forest, but the N storage had less difference. In the floor litter layer of the forests, Acari and Collembola were the dominant groups of soil arthropod community, while Diptera larvae, Coleoptera, ants, and Homoptera were the common groups. The Sorenson coefficients of soil arthropod community in the three forests were extremely great. No significant differences were observed in the soil arthropod density (ind x m(-2)) in the floor litter layer among the three forests, but the relative density (ind x g(-1)) of soil arthropods was higher in the evergreen broad-leaved forest and P. bonatii forest than in the mossy dwarf forest. In the three forests, the density of soil arthropods was significantly higher in dry season than in rainy season, but the Shannon diversity index had less difference. There were significant positive correlations between the existing floor litter mass and the individual density (ind x m(-2)) and dominant groups of soil arthropod communities in dry and hot season (April), but negative correlations between the existing floor litter mass and the relative density (ind x g(-1)) of soil arthropod communities and Acari in dry and cold season (December). The individual densities of Collembola and Coleoptera also had positive correlations with the N storage of the existing floor litter mass in the three forests. It was considered that the floor litter and the development of soil arthropod community in the litter layer of the subtropical forests in Ailao Mountain had a close relation with the vegetation structure of the forests, and the individual density and the diversity of the soil arthropod community were controlled by the floor litter, whereas the environmental factors such as temperature and moisture in the forests also had obvious effects on the seasonal dynamics of the individual density of the soil arthropods.

  1. Job Analysis Techniques for Restructuring Health Manpower Education and Training in the Navy Medical Department. Attachment 10. PT/OT QPCB Task Sort for Physical and Occupational Therapy.

    ERIC Educational Resources Information Center

    Technomics, Inc., McLean, VA.

    This publication is Attachment 10 of a set of 16 computer listed QPCB task sorts, by career level, for the entire Hospital Corps and Dental Technician fields. Statistical data are presented in tabular form for a detailed listing of job duties in physical and occupational therapy. (BT)

  2. An investigation of drag reduction fairings on the space shuttle vehicle 5 configuration (model 74-OTS) in the MSFC 14 inch trisonic wind tunnel (FA14)

    NASA Technical Reports Server (NTRS)

    Ramsey, P. E.

    1976-01-01

    An experimental investigation was conducted in the MSFC 14-inch TWT (FA14, TWT 600) to determine the static stability and drag on a 0.004 scale model of the shuttle ascent configuration. The primary objective was to study the possibility of reducing the launch vehicle drag by using Orbiter/ET/SRB fairings, streamlined orbiter fore and aft attach structures, SRB and ET alternative nose configurations, and devices for modifying the flow between the orbiter and ET. The secondary objective was to determine the longitudinal and directional characteristics of the ascent configuration with the most promising of the drag reduction devices installed. Data were obtained for a Mach number range of 0.6 through 4.96 and angles of attack from -5 through 5 degrees at zero degrees side slip angle.

  3. Space shuttle launch vehicle (13 P-OTS) strut support interference effects study in the Rockwell International 7- by 7-foot trisonic wind tunnel (IA68)

    NASA Technical Reports Server (NTRS)

    Rogge, R. L.

    1974-01-01

    Strut support interference investigations were conducted on an 0.004-(-) scale representation of the space shuttle launch vehicle in order to determine transonic and supersonic model support interference effects for use in a future exhaust plume effects study. Strut configurations were also tested. Orbiter, external tank, and solid rocket booster pressures were recorded at Mach numbers 0.9, 1.2, 1.5, and 2.0. Angle of attack and angle of sideslip were varied between plus or minus 4 degrees in 2 degree increments. Parametric variations consisted only of the strut configurations.

  4. 12 CFR 502.27 - How does OTS determine the risk/complexity component for a savings and loan holding company?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... will establish the amounts in column C and D in the Thrift Bulletin for each holding company risk/complexity classification. The amounts established for column C and D that are applicable to conglomerates will be three times the amounts established for column C and D for complex or higher risk...

  5. Aerodynamic results of wind tunnel separation tests on a 0.01-scale model (32-OTS) space shuttle integrated vehicle in the AEDC-VKF-SWTA (IA57)

    NASA Technical Reports Server (NTRS)

    Daileda, J. J.

    1974-01-01

    The integrated space shuttle vehicle was tested to determine proximity force and moment data for the orbiter/external tank and solid rocket booster (SRB) with and without separation rockets firing for both single and dual booster runs. Data were obtained at points t = 0 sec, t = 1.25 sec, and t = 2 sec on the nominal SRB separation trajectory.

  6. Study of structure-function relationships in proteins: Techniques and applications ot cytochrome c: Final report January 15, 1988--January 14, 1989

    SciTech Connect

    Goldstein, D.A.; Rackovsky, S.R.

    1989-08-01

    During the initial period of this work we explored the differential geometry results which had been used to explain the structure-function relationships in the set of yeast iso-1-cytochrome c mutants studied under the initial contract. In addition we continued the development of techniques which would permit the structural characterization and comparison of proteins in a very efficient manner. We have expanded the studies based on the characterization of the structural preferences of various residues in a sample of twenty six globular proteins. It has been demonstrated that the overall structural preferences and the amino acid specific preferences seen in the analysis carried out at the five alpha carbon level can not be explained by the results of the analysis carried out at the four alpha carbon level. Thus the structural preferences seen must be described by considering groups of five or more residues. We do no yet have enough data to extend the analysis to the six alpha carbon unit level. We have also verified that the yeast/tuna structural analogy which we used before was justified, and have performed a conformational energy minimization of the reduced yeast cytochrome c crystal data in order to have a baseline for the study of mutant proteins. 6 refs.

  7. OT1_gsacco_1: Characterizing the evolved, planet-forming disks orbiting the "old" classical T Tauri systems V4046 Sgr and MP Mus

    NASA Astrophysics Data System (ADS)

    Sacco, G.

    2010-07-01

    V4046 Sgr and MP Mus are the second and the third closest known classical (actively accreting) T Tauri systems, respectively (the intensively studied TW Hya being the closest). We have recently established that, like TW Hya, both systems are orbited by dusty, molecular disks. Given their proximity (d<100 pc), ages (~10 Myr), and masses (0.7-1.2 solar masses), these three systems represent readily-studied analogs of the young sun during the crucial, late phases of the star formation process, when the circumstellar disk still retains a significant gaseous component and giant planets likely are forming or have recently formed. Furthermore, the close binary V4046 Sgr affords an unusual opportunity to investigate planet formation within circumbinary disks. We propose to observe the V4046 Sgr and MP Mus disks using Herschel's PACS and SPIRE spectrometers with the complementary aims of (1) measuring the detailed spectral energy distribution of the continuum dust emission between 55 and 670 micron and (2) detecting the brightest atomic emission lines. These data, together with the comprehensive suite of X-ray, mid-infrared and radio observations we are already collecting for these two systems, will allow us to fully characterize the physical properties of both the dust and the gas components of their circumstellar disks and to investigate the effects of high energy emission from the central star on the evolution of the circumstellar, planet-forming environment.

  8. OT1_abaezrub_2: Unveiling the kinematics of the MWC49A's ionized disk and outflow with H-recombination line masers.

    NASA Astrophysics Data System (ADS)

    Báez-Rubio, A.

    2010-07-01

    % MWC349A, the unique H-maser source observed so far, is a massive star with an ionized outflow believed to be generated in its photoevaporating rotating circumstellar disk. We plan to use the unique spectral capabilities provided by HIFI to perform observations of basically all of the Hα recombination lines (from H26α to H15α) covered by this instrument. Spectral resolved profiles will reveal essential kinematics inormation of the inner regions of the ionized outflow and the rotating disk. The observed line profile will be compared with the prediction of our non-LTE 3D radiative transfer model to constrain key parameters of the disk-outflow system. From the model's prediction we will stablish if the inner disk is rotating with a Keplerian law, the rotation of the outflow and likely the presence of radial motions (accretion/excretion) in the disk and the location of the lunching point of the outflow. These parameters will help to discriminate between the models proposed for the origin of the outflows and the evolutionary state (pre-main sequence or evolved B[e] stars) of the central star.

  9. Wind tunnel test of the 0.019 scale space shuttle integrated vehicle (model 14-OTS) in the CALSPAN 8-foot transonic wind tunnel (IA36), volume 2

    NASA Technical Reports Server (NTRS)

    Hardin, R. B.; Burrows, R. R.

    1975-01-01

    A test is presented which was performed to determine the effect of cold jet gas plumes generated from main propulsion system and solid rocket motor nozzles on: (1) six-component force and moment data, (2) wing static pressures, (3) wing hinge moment, (4) elevon hinge moment, (5) rudder hinge moment, and (6) orbiter MPS nozzle pressure loads. The effects of rudder deflection, nozzle gimbal angle, and plume size were also obtained.

  10. INDUCTION OF GENOTOXIC DAMAGE IS NOT HIGHLY CORRELATED WITH THE ABILITY OT METHYLATE ARSENITE IN VITRO IN THE LEUKOCYTES OF 4 MAMMALIAN SPECIES

    EPA Science Inventory


    Abstract

    Arsenic is a natural drinking water contaminant that impacts the health of large populations of people throughout the world; however, the mode or mechanism by which arsenic induces cancer is unclear. In a series of in vitro studies, we exposed leukocytes from...

  11. Hypersonic aeroheating test of space shuttle vehicle: Configuration 3 (model 22 OTS) in the NASA-Ames 3.5-foot hypersonic wind tunnel (IH20), volume 2

    NASA Technical Reports Server (NTRS)

    Kingsland, R. B.; Lockman, W. K.

    1975-01-01

    The model tested was an 0.0175-scale version of the vehicle 3 space shuttle configuration. Temperature measurements were made on the launch configuration, orbiter plus tank, orbiter alone, tank alone, and solid rocket booster (SRB) alone to provide heat transfer data. The test was conducted at free stream Mach numbers of 5.3 and 7.3 and at free stream Reynolds numbers of 1.5, 3.7, 5.0, and 7.0 million per foot. The model was tested at angles of attack from -5 deg to 20 deg and side slip angles of -5 deg and 0 deg.

  12. Hypersonic aeroheating test of space shuttle vehicle configuration 3 (model 22-OTS) in the NASA-Ames 3.5-foot hypersonic wind tunnel (IH20), volume 1

    NASA Technical Reports Server (NTRS)

    Kingsland, R. B.; Lockman, W. K.

    1975-01-01

    The results of hypersonic wind tunnel testing of an 0.0175 scale version of the vehicle 3 space shuttle configuration are presented. Temperature measurements were made on the launch configuration, orbiter plus tank, orbiter alone, tank alone, and solid rocket booster alone to provide heat transfer data. The test was conducted at free-stream Mach numbers of 5.3 and 7.3 and at free-stream Reynolds numbers of 1.5 million, 3.7 million, 5.0 million, and 7.0 million per foot. The model was tested at angles of attack from -5 deg to 20 deg and side slip angles of -5 deg and 0 deg.

  13. 33 CFR 150.445 - When is oil in a single point mooring-oil transfer system (SPM-OTS) displaced with water?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... the pipeline end manifold must be closed whenever: (1) A storm warning forecasts weather conditions... vessel is about to depart the SPM because of storm conditions; or (3) The SPM is not scheduled for use...

  14. 33 CFR 150.445 - When is oil in a single point mooring-oil transfer system (SPM-OTS) displaced with water?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... the pipeline end manifold must be closed whenever: (1) A storm warning forecasts weather conditions... vessel is about to depart the SPM because of storm conditions; or (3) The SPM is not scheduled for use...

  15. 33 CFR 150.445 - When is oil in a single point mooring-oil transfer system (SPM-OTS) displaced with water?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... the pipeline end manifold must be closed whenever: (1) A storm warning forecasts weather conditions... vessel is about to depart the SPM because of storm conditions; or (3) The SPM is not scheduled for use...

  16. 33 CFR 150.445 - When is oil in a single point mooring-oil transfer system (SPM-OTS) displaced with water?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... the pipeline end manifold must be closed whenever: (1) A storm warning forecasts weather conditions... vessel is about to depart the SPM because of storm conditions; or (3) The SPM is not scheduled for use...

  17. 33 CFR 150.445 - When is oil in a single point mooring-oil transfer system (SPM-OTS) displaced with water?

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... the pipeline end manifold must be closed whenever: (1) A storm warning forecasts weather conditions... vessel is about to depart the SPM because of storm conditions; or (3) The SPM is not scheduled for use...

  18. OT2_alopezse_3: Mapping the cosmic ray ionisation rate across the Northern end of the Orion A iant molecular cloud

    NASA Astrophysics Data System (ADS)

    López-Sepulcre, A.

    2011-09-01

    Cosmic rays (CR) are ubiquitous in the Galaxy and have the important role of ionizing the dens gas of the ISM. New Herschel observations have shown the huge diagnostic power of the OH+ fundamental transition to measure the CR ionization rate in diffuse clouds. Based on previous "serendipity" observations toward OMC2-FIR4 within the KP CHESS, we discovered a tenuous foreground cloud absorbing the fundamental OH+ line. Similarly, Gupta et al. (2010) found an OH+ absorption component at a similar velocity towards Orion KL and estimated a large CR ionization rate more than 10 times larger than the average value observed in diffuse clouds . We propose here to roughly map the CR ionization rate in the direction of the OMC2 and OMC3 complex to understand its extent, nature, and, finally, the source of ionization.

  19. Laboratory and outside the laboratory measurements of ginned and ot ginned cotton for fiber micronaire and maturity by portable Near Infrared (NIR) Instruments

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Micronaire is a key cotton quality assessment property, impacting downstream fiber processing and dye consistency. A component of micronaire is fiber maturity (degree of secondary wall development). Historically, micronaire and maturity are measured in a laboratory under tight environmental condit...

  20. OT1_smolinar_5: Hi-GAL360: the crucial step toward a global understanding of star formation in the Milky Way

    NASA Astrophysics Data System (ADS)

    Molinari, S.

    2010-07-01

    Hi-GAL360 will use PACS and SPIRE in parallel mode to obtain a 5-band photometric survey of the Outer Galactic Plane (OGP, beyond the solar circle) in the longitude range complementary to the Hi-GAL Open Time KP, i.e., 68 < l < 288 in a 2-wide strip in latitude following the mid-plane of emission. Hi-GAL360 data will enable a complete census of temperature, mass, density, column density and luminosity of filaments, clumps, and cores in the less confused outer Galaxy, where the assembly of filamentary structures and clouds and their fragmentation into clumps and cores can be uniquely characterised in low-metallicity and HI-dominated environments that are so different with respect to the inner Galaxy. A complete OGP survey is required to obtain a statistically significant sampling of Galactic diversity - the full range of spiral arm, inter-arm, dust cloud, and star formation region properties - avoiding biases introduced by the study of specific or limited regions. Hi-GAL360, together with Hi-GAL, will chart the Star Formation Rate and Efficiency from the Galactic Center to the far outer Galaxy, mapping the location and properties of star formation thresholds, thus providing the much needed connection between global scaling laws and the diversity of physical processes at work in the Galaxy. It will lay the foundations for a predictive, quantitative model of how star formation is triggered and regulated on all scales in the Milky Way. Such a model is vital to a full understanding of galaxy formation and evolution. The merged Hi-GAL360/Hi-GAL `Atlas of the Galaxy' will provide essential `ground truth' for the interpretation of Herschel data on other galaxies from the Local Group to the high-redshift Universe where these objects are blended, and it will be the Herschel legacy that will remain unsurpassed for decades as the definitive survey of far-IR emission from the Galaxy. Given our demonstrated community-oriented approach, we again waive our proprietary period.

  1. Method of Transfer from Logical Schemes of Algorithms to Boolean Functions Ot Logicheskikh Skhem Algoritmov K Bulevym Funktsiyam<

    NASA Technical Reports Server (NTRS)

    Dyachenko, V. F.

    1964-01-01

    The author discusses a method for obtaining the Boolean functions describing the structure of a control circuit by means of AS operators whose sequence of operations is given in the form of a logical scheme or matrix scheme for an algorithm. An example of AS synthesis is given.

  2. A New Population of Parvocellular Oxytocin Neurons Controlling Magnocellular Neuron Activity and Inflammatory Pain Processing.

    PubMed

    Eliava, Marina; Melchior, Meggane; Knobloch-Bollmann, H Sophie; Wahis, Jérôme; da Silva Gouveia, Miriam; Tang, Yan; Ciobanu, Alexandru Cristian; Triana del Rio, Rodrigo; Roth, Lena C; Althammer, Ferdinand; Chavant, Virginie; Goumon, Yannick; Gruber, Tim; Petit-Demoulière, Nathalie; Busnelli, Marta; Chini, Bice; Tan, Linette L; Mitre, Mariela; Froemke, Robert C; Chao, Moses V; Giese, Günter; Sprengel, Rolf; Kuner, Rohini; Poisbeau, Pierrick; Seeburg, Peter H; Stoop, Ron; Charlet, Alexandre; Grinevich, Valery

    2016-03-16

    Oxytocin (OT) is a neuropeptide elaborated by the hypothalamic paraventricular (PVN) and supraoptic (SON) nuclei. Magnocellular OT neurons of these nuclei innervate numerous forebrain regions and release OT into the blood from the posterior pituitary. The PVN also harbors parvocellular OT cells that project to the brainstem and spinal cord, but their function has not been directly assessed. Here, we identified a subset of approximately 30 parvocellular OT neurons, with collateral projections onto magnocellular OT neurons and neurons of deep layers of the spinal cord. Evoked OT release from these OT neurons suppresses nociception and promotes analgesia in an animal model of inflammatory pain. Our findings identify a new population of OT neurons that modulates nociception in a two tier process: (1) directly by release of OT from axons onto sensory spinal cord neurons and inhibiting their activity and (2) indirectly by stimulating OT release from SON neurons into the periphery.

  3. A New Population of Parvocellular Oxytocin Neurons Controlling Magnocellular Neuron Activity and Inflammatory Pain Processing.

    PubMed

    Eliava, Marina; Melchior, Meggane; Knobloch-Bollmann, H Sophie; Wahis, Jérôme; da Silva Gouveia, Miriam; Tang, Yan; Ciobanu, Alexandru Cristian; Triana del Rio, Rodrigo; Roth, Lena C; Althammer, Ferdinand; Chavant, Virginie; Goumon, Yannick; Gruber, Tim; Petit-Demoulière, Nathalie; Busnelli, Marta; Chini, Bice; Tan, Linette L; Mitre, Mariela; Froemke, Robert C; Chao, Moses V; Giese, Günter; Sprengel, Rolf; Kuner, Rohini; Poisbeau, Pierrick; Seeburg, Peter H; Stoop, Ron; Charlet, Alexandre; Grinevich, Valery

    2016-03-16

    Oxytocin (OT) is a neuropeptide elaborated by the hypothalamic paraventricular (PVN) and supraoptic (SON) nuclei. Magnocellular OT neurons of these nuclei innervate numerous forebrain regions and release OT into the blood from the posterior pituitary. The PVN also harbors parvocellular OT cells that project to the brainstem and spinal cord, but their function has not been directly assessed. Here, we identified a subset of approximately 30 parvocellular OT neurons, with collateral projections onto magnocellular OT neurons and neurons of deep layers of the spinal cord. Evoked OT release from these OT neurons suppresses nociception and promotes analgesia in an animal model of inflammatory pain. Our findings identify a new population of OT neurons that modulates nociception in a two tier process: (1) directly by release of OT from axons onto sensory spinal cord neurons and inhibiting their activity and (2) indirectly by stimulating OT release from SON neurons into the periphery. PMID:26948889

  4. Data report for tests on the heat transfer effects of the 0.0175-scale Rockwell International Space Shuttle Vehicle model 22-OT in the AEDC 50-inch B wind tunnel (OH4B), volume 3

    NASA Technical Reports Server (NTRS)

    Foster, T. F.; Grifall, W. J.; Martindale, W.

    1975-01-01

    Results of wind tunnel heat transfer tests of 0.0175-scale Rockwell International Space Shuttle Vehicle configurations for orbiter alone, tank alone, and orbiter plus external tank are presented. Body flap shielding of SSME's during simulated entry was also investigated. The tests were conducted at Mach 8 for thirteen Reynolds number per foot values ranging from 0.5 million to 3.72 million.

  5. Data report for tests on the heat transfer effects of the 0.0175 scale Rockwell International Space Shuttle Vehicle model 22-OT in the AEDC 50 inch B wind tunnel (0H4B), volume 1

    NASA Technical Reports Server (NTRS)

    Foster, T. F.; Grifall, W. J.; Martindale, W.

    1975-01-01

    Results of wind tunnel heat transfer tests of 0.0175-scale Rockwell International Space Shuttle Vehicle configurations for orbiter alone, tank alone, and orbiter plus external tank are presented. Body flap shielding of SSME's during simulated entry was investigated. The tests were conducted at Mach 8 for thirteen Reynolds number.

  6. Results of an investigation of Reynolds effects on integrated vehicle elevon hinge moments and wing panel loads obtained with 0.010 scale model 72 OTS in the Rockwell trisonic wind tunnel (IA141)

    NASA Technical Reports Server (NTRS)

    Mennell, R. C.

    1976-01-01

    Wind tunnel investigations were conducted on an 0.010-scale representation of the VL70-000140C Integrated Space Shuttle Launch Vehicle. The primary test objective was to obtain Reynolds number effects on orbiter elevon hinge moments and wing bending/torsional moments. Launch vehicle aerodynamic force data were also recorded. The elevon hinge moments, wing bending/torsional moments, and vehicle force data were recorded over an angle of attack range of -6 deg to +6 deg, an angle of sideslip range of -6 deg to +6 deg, at Mach numbers of 0.6, 0.975, 1.05 and 1.25. The Reynolds number was varied from a minimum of 4.5 million/foot to a maximum of 11.5 million/foot. The complete integrated configuration was tested with the orbiter elevons set at 0 deg and deflected to 9 deg on the outboard elevon and 10 deg on the inboard elevon. Testing was conducted in the TWT 19.7% porous transonic test section with the model sting mounted through the orbiter base. All aerodynamic force data were obtained from internal strain gage balance located in the orbiter.

  7. Wind tunnel tests of an 0.019-scale space shuttle integrated vehicle -2A configuration (model 14-OTS) in the NASA Ames 8 by 7 foot unitary wind tunnel (IA12C), volume 1. [torque and exhaust gases

    NASA Technical Reports Server (NTRS)

    Hardin, R. B.; Burrows, R. R.

    1975-01-01

    The purpose of the test was to determine the effects of cold jet gas plumes on (1) the integrated vehicle longitudinal and lateral-directional force data, (2) exposed wing hinge moment, (3) wing pressure distributions, (4) orbiter MPS external pressure distributions, and (5) model base pressure. The similarity between solid and gaseous plumes was investigated, and fluorescent oil flow visualization studies were conducted.

  8. Innovative Clean Coal Technology (ICCT). Demonstration of Selective Catalytic Reduction (SCR) technology for the control of nitrogen oxide (NO{sub x}) emissions from high-sulfur coal-fired boilers: Volume 3, Appendices O--T. Final report

    SciTech Connect

    1996-10-01

    Volume 3 contains the following appendices: Appendix O, Second Series-Manual APH Tests; Appendix P, Third Series-Manual APH Tests; Appendix Q, ABB Analysis of Air Preheaters-Final Report; Appendix R, ABB Corrosion Analysis Study; Appendix S, SRI Waste Stream Impacts Study; and Appendix T, Economic Evaluation.

  9. Results of investigations conducted in the LaRC 8-foot transonic pressure tunnel using the 0.010-scale 72-OTS model of the space shuttle integrated vehicle (IA93), volume 2

    NASA Technical Reports Server (NTRS)

    Nichols, M. E.

    1976-01-01

    Test procedures, history, and plotted coefficient data are presented for an aero-loads investigation on the updated configuration-5 space shuttle launch vehicle at Mach numbers from 0.600 to 1.205. Six-component vehicle forces and moments, base and sting-cavity pressures, elevon hinge moments, wing-root bending and torsion moments, and normal shear force data were obtained. Full simulation of updated vehicle protuberances and attach hardware was employed.

  10. An investigation in MSFC 14-inch TWT to determine the static stability characteristics of 0.004-scale model (74-OTS) space shuttle vehicle 5 configuration (IA33), volume 1

    NASA Technical Reports Server (NTRS)

    Allen, E. C.

    1975-01-01

    Wind tunnel tests were conducted to: (1) determine the static stability characteristics of the Shuttle Vehicle 5 configuration; (2) determine the effect on the Vehicle 5 aerodynamic characteristics of External Tank (ET) and Solid Rocket Booster (SRB) nose shape, SRB nozzle shroud flare angle, orbiter to tank fairing, and sting location; (3) provide flow visualization using thin film oil paint; and (4) determine rudder, body flap, and inboard and outboard elevon hinge moments. The mated vehicle model was mounted in three different ways: (1) the orbiter mounted on the balance with the SRB's attached to the tank and the tank in turn attached to the orbiter; (2) the tank mounted on the balance (with the sting protruding through the tank base) with the SRB's and orbiter attached to the tank, and (3) with the tank mounted on the balance and the balance in turn supported by a forked sting entering the nozzle of each SRB, extending forward into the SRB's then crossing over to the tank to provide a balance socket. Data were obtained for Mach numbers from 0.6 through 4.96 at angles-of-attack and -sideslip from -10 to 10 degrees.

  11. Results of transonic wind tunnel tests on an 0.015-scale space shuttle mated vehicle model (67-ots) in the LaRC 8 foot TPT (IA41)

    NASA Technical Reports Server (NTRS)

    Hardin, R.; Burrows, R. R.

    1974-01-01

    Wind tunnel tests were conducted to obtain aerodynamic force data for Mach numbers from 0.60 to 1.20. Data were obtained for an alpha range of -10 deg to +10 deg (beta = 0 deg beta = 5 deg) and beta range of -10 deg to +10 deg (alpha = 0 deg). Longitudinal and lateral-directional stability and control data were obtained for tank alone, tank plus SRB's, tank plus Orbiter, and mated configuration of tank + Orbiter + SRB's. Also, single-component rudder hinge moment data were obtained at rudder deflections of 0 and -20 deg for each Mach number tested. Plots of aerodynamic coefficients vs. Mach number are presented, using data from both test IA41 and tests LRC-UPWT-1056, 1073 (IA42A/B) for Mach numbers of 1.60 to 4.63. The model tested in IA42A/B was the same model as tested in IA41.

  12. Results of investigations of an 0.010-scale 140A/B configuration (model 72-OTS) of the Rockwell International space shuttle orbiter in the NASA/Langley Research Center unitary plan wind tunnel

    NASA Technical Reports Server (NTRS)

    Petrozzi, M. T.; Milam, M. D.

    1975-01-01

    Experimental aerodynamic investigations were conducted in the NASA/Langley unitary plan wind tunnel on a sting mounted 0.010-scale outer mold line model of the 140A/B configuration of the Rockwell International Space Shuttle Vehicle. The primary test objectives were to obtain: (1) six component force and moment data for the mated vehicle at subsonic and transonic conditions, (2) effects of configuration build-up, (3) effects of protuberances, ET/orbiter fairings and attach structures, and (4) elevon deflection effects on wing bending moment. Six component aerodynamic force and moment data and base and balance cavity pressures were recorded over Mach numbers of 1.6, 2.0, 2.5, 2.86, 3.9, and 4.63 at a nominal Reynolds number of 20 to the 6th power per foot. Selected configurations were tested at angles of attack and sideslip from -10 deg to +10 deg. For all configurations involving the orbiter, wing bending, and torsion coefficients were measured on the right wing.

  13. Results of wind tunnel tests of an ASRM configured 0.03 scale Space Shuttle integrated vehicle model (47-OTS) in the AEDC 16-foot Transonic wind tunnel (IA613A), volume 1

    NASA Astrophysics Data System (ADS)

    Marroquin, J.; Lemoine, P.

    1992-10-01

    An experimental Aerodynamic and Aero-Acoustic loads data base was obtained at transonic Mach numbers for the Space Shuttle Launch Vehicle configured with the ASRM Solid Rocket Boosters as an increment to the current flight configuration (RSRB). These data were obtained during transonic wind tunnel tests (IA 613A) conducted in the Arnold Engineering Development Center 16-Foot transonic propulsion wind tunnel from March 27, 1991 through April 12, 1991. This test is the first of a series of two tests covering the Mach range from 0.6 to 3.5. Steady state surface static and fluctuating pressure distributions over the Orbiter, External Tank and Solid Rocket Boosters of the Shuttle Integrated Vehicle were measured. Total Orbiter forces, Wing forces and Elevon hinge moments were directly measured as well from force balances. Two configurations of Solid Rocket Boosters were tested, the Redesigned Solid Rocket Booster (RSRB) and the Advanced Solid Rocket Motor (ASRM). The effects of the position (i.e. top, bottom, top and bottom) of the Integrated Electronics Assembly (IEA) box, mounted on the SRB attach ring, were obtained on the ASRM configured model. These data were obtained with and without Solid Plume Simulators which, when used, matched as close as possible the flight derived pressures on the Orbiter and External Tank base. Data were obtained at Mach numbers ranging from 0.6 to 1.55 at a Unit Reynolds Number of 2.5 million per foot through model angles of attack from -8 to +4 degrees at sideslip angles of 0, +4 and -4 degrees.

  14. Results of wind tunnel tests of an ASRM configured 0.03 scale Space Shuttle integrated vehicle model (47-OTS) in the AEDC 16-foot transonic wind tunnel, volume 2

    NASA Astrophysics Data System (ADS)

    Marroquin, J.; Lemoine, P.

    1992-10-01

    An experimental Aerodynamic and Aero-Acoustic loads data base was obtained at transonic Mach numbers for the Space Shuttle Launch Vehicle configured with the ASRM Solid Rocket Boosters as an increment to the current flight configuration (RSRB). These data were obtained during transonic wind tunnel tests (IA 613A) conducted in the Arnold Engineering Development Center 16-Foot transonic propulsion wind tunnel from March 27, 1991 through April 12, 1991. This test is the first of a series of two tests covering the Mach range from 0.6 to 3.5. Steady state surface static and fluctuating pressure distributions over the Orbiter, External Tank and Solid Rocket Boosters of the Shuttle Integrated Vehicle were measured. Total Orbiter forces, Wing forces and Elevon hinge moments were directly measured as well from force balances. Two configurations of Solid Rocket Boosters were tested, the Redesigned Solid Rocket Booster (RSRB) and the Advanced Solid Rocket Motor (ASRM). The effects of the position (i.e., top, bottom, top and bottom) of the Integrated Electronics Assembly (IEA) box, mounted on the SRB attach ring, were obtained on the ASRM configured model. These data were obtained with and without Solid Plume Simulators which, when used, matched as close as possible the flight derived pressures on the Orbiter and External Tank base. Data were obtained at Mach numbers ranging from 0.6 to 1.55 at a Unit Reynolds Number of 2.5 million per foot through model angles of attack from -8 to +4 degrees at sideslip angles of 0, +4 and -4 degrees.

  15. Heat transfer tests of an 0.006-scale thin-skin space shuttle thermocouple model (141-OT) in the Langley Research Center Freon tunnel at M-6 (IH18). [wind tunnel tests

    NASA Technical Reports Server (NTRS)

    Walstad, D. G.

    1976-01-01

    Ascent heating data were obtained at conditions simulating real gas effects at hypersonic Mach numbers. The configurations tested were Orbiter alone, external tank alone, and mated Orbiter and external tank. A boundary layer trip investigation was conducted for all configurations. The test was conducted at Mach 6 and Reynolds number of one half million per foot for 0 deg and -5 deg angle-of-attack. Selected thermocouples were chosen from the Orbiter and external tank to be used for obtaining heat transfer measurements. A maximum of 42 thermocouples could be measured by the facility data acquisition at one time and no attempt was made to record the excess thermocouples located on the model. Photographs of the test configurations are shown.

  16. Results of wind tunnel tests of an ASRM configured 0.03 scale Space Shuttle integrated vehicle model (47-OTS) in the AEDC 16-foot transonic wind tunnel, volume 2

    NASA Technical Reports Server (NTRS)

    Marroquin, J.; Lemoine, P.

    1992-01-01

    An experimental Aerodynamic and Aero-Acoustic loads data base was obtained at transonic Mach numbers for the Space Shuttle Launch Vehicle configured with the ASRM Solid Rocket Boosters as an increment to the current flight configuration (RSRB). These data were obtained during transonic wind tunnel tests (IA 613A) conducted in the Arnold Engineering Development Center 16-Foot transonic propulsion wind tunnel from March 27, 1991 through April 12, 1991. This test is the first of a series of two tests covering the Mach range from 0.6 to 3.5. Steady state surface static and fluctuating pressure distributions over the Orbiter, External Tank and Solid Rocket Boosters of the Shuttle Integrated Vehicle were measured. Total Orbiter forces, Wing forces and Elevon hinge moments were directly measured as well from force balances. Two configurations of Solid Rocket Boosters were tested, the Redesigned Solid Rocket Booster (RSRB) and the Advanced Solid Rocket Motor (ASRM). The effects of the position (i.e., top, bottom, top and bottom) of the Integrated Electronics Assembly (IEA) box, mounted on the SRB attach ring, were obtained on the ASRM configured model. These data were obtained with and without Solid Plume Simulators which, when used, matched as close as possible the flight derived pressures on the Orbiter and External Tank base. Data were obtained at Mach numbers ranging from 0.6 to 1.55 at a Unit Reynolds Number of 2.5 million per foot through model angles of attack from -8 to +4 degrees at sideslip angles of 0, +4 and -4 degrees.

  17. Wind tunnel tests of an 0.019-scale space shuttle integrated vehicle -2A configuration (model 14-OTS) in the NASA Ames 8 X 7 foot unitary wind tunnel, volume 3. [cold jet gas plumes and pressure distribution

    NASA Technical Reports Server (NTRS)

    Hardin, R. B.; Burrows, R. R.

    1975-01-01

    Tests were conducted to determine the effects of cold jet gas plumes on (1) the integrated vehicle longitudinal and lateral-directional force data, (2) exposed wing hinge moment, (3) wing pressure distributions, (4) orbiter MPS external pressure distributions, and (5) model base pressures. An investigation was undertaken to determine the similarity between solid and gaseous plumes; fluorescent oil flow visualization studies were also conducted. Tabulated data listings are included.

  18. Results of experimental investigations to determine external tank protuberance loads using a 0.03-scale model of the Space Shuttle launch configuration (model 47-OTS) in the NASA/ARC unitary plan wind tunnel, volume 1

    NASA Technical Reports Server (NTRS)

    Houlihan, S. R.

    1992-01-01

    Data were obtained on a 3-percent model of the Space Shuttle launch vehicle in the NASA/Ames Research Center 11x11-foot and 9x7-foot Unitary Plan Wind Tunnels. This test series has been identified as IA190A/B and was conducted from 7 Feb. 1980 to 19 Feb. 1980 (IA190A) and from 17 March 1980 to 19 March 1980 and from 8 May 1980 to 30 May 1980 (IA190B). The primary test objective was to obtain structural loads on the following external tank protuberances: (1) LO2 feedline, (2) GO2 pressure line, (3) LO2 antigeyser line, (4) GH2 pressure line, (5) LH2 tank cable tray, (6) LO2 tank cable tray, (7) Bipod, (8) ET/SRB cable tray, and (9) Crossbeam/Orbiter cable tray. To fulfill these objectives the following steps were taken: (1) Eight 3-component balances were used to measure forces on various sections of 1 thru 6 above. (2) 315 pressure orifices were distributed over all 9 above items. The LO2 feedline was instrumented with 96 pressure taps and was rotated to four positions to yield 384 pressure measurements. The LO2 antigeyser line was instrumented with 64 pressure taps and was rotated to two positions to yield 128 pressure measurements. (3) Three Chrysler miniature flow direction probes were mounted on a traversing mechanism on the tank upper surface centerline to obtain flow field data between the forward and aft attach structures. (4) Schlieren photographs and ultraviolet flow photographs were taken at all test conditions. Data from each of the four test phases are presented.

  19. Results of transonic tests in the NASA/MSFC 14-inch trisonic wind tunnel on a 0.004-scale model (74-OTS) space shuttle launch vehicle (FA25)

    NASA Technical Reports Server (NTRS)

    Lundy, T. E.

    1979-01-01

    The primary objective of the test was to determine the aerodynamic increments due to the attach structure. Secondary objectives were to determine the effects of: (1) orbiter nose mold line changes; (2) wire bundle fairings on data measurements; and (3) flow angularity. The scale model was tested over the Mach range from 0.60 to 1.25 at angles of attack and sideslip from -8 to +8 deg. The total pressure was 22 psia for all runs. Six-component orbiter data were obtained from a balance in the orbiter which was sting supported. The external tank was attached to the solid rocket booster, each of which was sting supported. An alternate two sting/two balance arrangement was also tested with a single sting and balance in the external tank measuring combined ET/SRB aero data replacing the two stings in the SRB's. Two runs were also made at Mach number 4.96 with the two SRB's removed. The aerodynamic coefficients obtained are tabulated as a function of angle of attack or sideslip for each Mach number value.

  20. Results of an external tank separation test in AEDC/VKF tunnel B on 0.010-scale replica of space shuttle vehicle model 52-OT(IA17A), Volume 1

    NASA Technical Reports Server (NTRS)

    Spangler, R. H.; Daileda, J. J.

    1975-01-01

    Tests were conducted on scale models of the space shuttle orbiter and external tank (ET) to determine the aerodynamic interactions during a return to launch site abort separation. The orbiter model was built to vehicle 3 configuration lines (139B) and the ET model approximated the vehicle 5 configurations with protuberances and attach hardware. For these investigations the orbiter was mounted on the primary support system and the external tank was mounted on the captive trajectory system. Six-component data were obtained for each vehicle at various orbiter angles of attack and sideslip for a range of relative angular and linear displacements of the ET from the orbiter.

  1. Results of Experimental Investigations to Determine External Tank Protuberance Loads Using a 0.03-Scale Model of the Space Shuttle Launch Configuration (Model 47-OTS) in the NASA/ARC Unitary Plan Wind Tunnel, Volume 2

    NASA Technical Reports Server (NTRS)

    Houlihan, S. R.

    1992-01-01

    Data were obtained on a 3-percent model of the Space Shuttle launch vehicle in the NASA/Ames Research Center 11x11-foot and 9x7-foot Unitary Plan Wind Tunnels. This test series has been identified as IA19OA/B and was conducted from 7 Feb. 1980 to 19 Feb. 1980 (IA19OA) and from 17 March 1980 to 19 March 1980 and from 8 May 1980 to 30 May 1980 (IA19OB). The primary test objective was to obtain structural loads on the following external tank protuberances: (1) LO2 feedline; (2) GO2 pressure line; (3) LO2 antigeyser line; (4) GH2 pressure line; (5) LH2 tank cable tray; (6) LO2 tank cable tray; (7) Bipod; (8) ET/SRB cable tray; and (9) Crossbeam/Orbiter cable tray. To fulfill these objectives the following steps were taken: Eight 3-component balances were used to measure forces on various sections of 1 thru 6 above; 315 pressure orifices were distributed over all 9 above items. The LO2 feedline was instrumented with 96 pressure taps and was rotated to four positions to yield 384 pressure measurements. The LO2 antigeyser line was instrumented with 64 pressure taps and was rotated to two positions to yield 128 pressure measurements; Three Chrysler miniature flow direction probes were mounted on a traversing mechanism on the tank upper surface centerline to obtain flow field data between the forward and aft attach structures; and Schlieren photographs and ultraviolet flow photographs were taken at all test conditions. Data from each of the four test phases are presented.

  2. Results of oil flow visualization tests of an 0.010-scale model (52-OT) of the space shuttle orbiter-tank mated and orbiter configurations in the AEDC VKF tunnel B (IA17B)

    NASA Technical Reports Server (NTRS)

    Daileda, J. J.

    1975-01-01

    An 0.010-scale model of the space shuttle (orbiter-tank mated and orbiter configurations) was tested in the AEDC VKF Tunnel B to investigate aerodynamic flow patterns. The tests utilized oil flow techniques to visualize the flow patterns. Tunnel free stream Mach number was 7.95 and nominal unit Reynolds number was 3.7 million per foot. Model angle of attack was varied from -5 deg through 10 deg and angle of sideslip was 0 deg and 2 deg. Photographs of resulting oil flow patterns are presented.

  3. Results of heat transfer tests of an 0.0175-scale space shuttle vehicle model 22 OTS in the NASA-Ames 3.5-foot hypersonic wind tunnel (IH3), volume 3

    NASA Technical Reports Server (NTRS)

    Foster, T. F.; Lockman, W. K.

    1975-01-01

    Heat-transfer data for the 0.0175-scale space shuttle vehicle 3 are presented, and interference heating effects were investigated by a model build-up technique of the orbiter. The test program was conducted at Mach 5.3 for nominal free-stream Reynolds number per foot values of 1,500,000 and 5,000,000.

  4. Results of heat transfer tests of an 0.0175-scale space shuttle vehicle model 22 OTS in the NASA-Ames 3.5-foot hypersonic wind tunnel (IH3), volume 4

    NASA Technical Reports Server (NTRS)

    Foster, T. F.; Lockman, W. K.

    1975-01-01

    Heat-transfer data for the 0.0175-scale Space Shuttle Vehicle 3 are presented. Interference heating effects were investigated by a model build-up technique of Orbiter alone, tank alone, second, and first stage configurations. The test program was conducted in the NASA-Ames 3.5-Foot Hypersonic Wind Tunnel at Mach 5.3 for nominal free-stream Reynolds number per foot values of 1.5 x 1,000,000 and 5.0 x 1,000,000.

  5. Results of heat transfer tests of a 0.0175-scale space shuttle vehicle 5 model (60-OTS) in the NASA-Ames Research Center 3.5-foot hypersonic wind tunnel (test IH48)

    NASA Technical Reports Server (NTRS)

    Dye, W. H.; Lockman, W. K.

    1976-01-01

    Heat transfer data are presented for a .0175-scale model of the Rockwell International Space Shuttle Vehicle 5. The primary purpose of these tests was to obtain aerodynamic interference heating data on the external tank in the tank alone, second-, and first-stage configurations. Data were also obtained on the Orbiter and solid rocket boosters. Nominal Mach Nos. of 5.2 and 5.3 at nominal freestream unit Reynolds numbers of 1.5 and 5.0 million per foot, respectively, were investigated. Photographs of the tested configurations and test equipment are shown.

  6. Results of heat transfer tests of an 0.0175-scale space shuttle vehicle model 22 OTS in the NASA-Ames 3.5 foot hypersonic wind tunnel (IH3), volume 1

    NASA Technical Reports Server (NTRS)

    Foster, T. F.; Lockman, W. K.

    1975-01-01

    Heat transfer data for the 0.0175-scale space shuttle vehicle 3 are presented. Interference heating effects were investigated by a model build-up technique of orbiter alone, tank alone, second, and first stage configurations. The test program was conducted in the NASA-Ames 3.5-foot hypersonic wind tunnel at Mach 5.3 for nominal free stream Reynolds number per foot values of 1.5, and 5.0 million.

  7. Results of wind tunnel tests of an ASRM configured 0.03 scale Space Shuttle integrated vehicle model (47-OTS) in the AEDC 16-foot Transonic wind tunnel (IA613A), volume 1

    NASA Technical Reports Server (NTRS)

    Marroquin, J.; Lemoine, P.

    1992-01-01

    An experimental Aerodynamic and Aero-Acoustic loads data base was obtained at transonic Mach numbers for the Space Shuttle Launch Vehicle configured with the ASRM Solid Rocket Boosters as an increment to the current flight configuration (RSRB). These data were obtained during transonic wind tunnel tests (IA 613A) conducted in the Arnold Engineering Development Center 16-Foot transonic propulsion wind tunnel from March 27, 1991 through April 12, 1991. This test is the first of a series of two tests covering the Mach range from 0.6 to 3.5. Steady state surface static and fluctuating pressure distributions over the Orbiter, External Tank and Solid Rocket Boosters of the Shuttle Integrated Vehicle were measured. Total Orbiter forces, Wing forces and Elevon hinge moments were directly measured as well from force balances. Two configurations of Solid Rocket Boosters were tested, the Redesigned Solid Rocket Booster (RSRB) and the Advanced Solid Rocket Motor (ASRM). The effects of the position (i.e. top, bottom, top and bottom) of the Integrated Electronics Assembly (IEA) box, mounted on the SRB attach ring, were obtained on the ASRM configured model. These data were obtained with and without Solid Plume Simulators which, when used, matched as close as possible the flight derived pressures on the Orbiter and External Tank base. Data were obtained at Mach numbers ranging from 0.6 to 1.55 at a Unit Reynolds Number of 2.5 million per foot through model angles of attack from -8 to +4 degrees at sideslip angles of 0, +4 and -4 degrees.

  8. Heat transfer tests on a 0.01-scale Rockwell configuration 3 space shuttle orbiter and tank (37-OT) in the Calspan 48-inch hypersonic shock tunnel (OH12/IH21), volume 1

    NASA Technical Reports Server (NTRS)

    Kotch, M.

    1975-01-01

    Model information and data are presented from wind tunnel tests conducted on 0.01-scale models of the space shuttle orbiter and external tank. These tests were conducted in a hypersonic shock tunnel to determine heating rates on ascent and reentry configurations at various Reynolds numbers, Mach numbers, and angles of attack.

  9. Aeroheating (pressure) characteristics on a 0.10-scale version of the vehicle 3 space shuttle configuration (26-OTS) in the Langley Research Center 4-foot wind tunnel (IH4)

    NASA Technical Reports Server (NTRS)

    Kingsland, R. B.

    1976-01-01

    Results of wind tunnel tests, conducted at the Langley Research Center Unitary Plan Wind Tunnel, are presented. The model tested was an 0.010-scale version of the Vehicle 3 Space Shuttle Configuration. Pressure measurements were made on the launch configuration, Orbiter alone, external tank alone, and solid rocket booster alone, to provide heat transfer pressure data. The tests were conducted for a Mach number range from 2.36 to 4.6 and Reynolds number range from 1.2 to 5 million per foot. The model was tested at angles of attack from -10 to 20 deg for a sideslip angle range from -5 to +5 deg, and at sideslip angles from -5 to 48 deg for 0 deg angle of attack. Tabulated data are given and photographs of the test configuration are shown.

  10. Results from investigations in the NASA/MSFC TWT on a .004 scale model space shuttle launch vehicle (model 13P-OTS) to determine gas supply strut effects on model pressure environment (IA53)

    NASA Technical Reports Server (NTRS)

    Garton, W. P.

    1975-01-01

    The Rockwell Space Shuttle Launch Vehicle components were tested in a Trisonic Wind Tunnel. Pressure measurements were made on the aft portion, in the base regions, and on the wing surfaces. Axial force determination were made from the pressure data. Data were recorded with the model at zero degrees angle of attack and sideslip through a Mach number from 0.9 to 3.0. The only configuration changes investigated were strut mounting techniques and gas line fairing location between the external tank and the orbiter.

  11. Flutter tests (IS4) of the 0.0125-scale shuttle reflection plane model 30-OTS in the Langley Research Center 26-inch transonic blowdown tunnel test no. 547

    NASA Technical Reports Server (NTRS)

    Kotch, M. A.

    1974-01-01

    A series of slab wing flutter models with rigid orbiter fuselage, external tank, and SRB models of the space shuttle were tested, in a reflection plane arrangement, in the NASA Langley Research Center's 26-inch Transonic Blowdown Tunnel. Model flutter boundaries were obtained for both a wing-alone configuration and a wing-with-orbiter, tank and SRB configuration. Additional test points were taken of the wing-with-orbiter configuration, as a correlation with the wing-alone condition. A description of the wind tunnel models and test procedures utilized in the experiment are provided.

  12. Results of investigations conducted in the LaRC 8-foot transonic pressure tunnel using the 0.010-scale 72-OTS model of the space shuttle integrated vehicle (IA93)

    NASA Technical Reports Server (NTRS)

    Nichols, M. E.

    1976-01-01

    Test procedures, history, and data from the wind tunnel test are presented. Aero-loads were investigated on the updated configuration-5 space shuttle launch vehicle at Mach numbers from 0.600 to 1.205. Six-component vehicle forces and moments, base and sting-cavity pressures, elevon hinge moments, wing-root bending and torsion moments, and normal shear force data were obtained. Full simulation of updated vehicle protuberances and attach hardware was employed. Various elevon deflection angles were tested with two different forward orbiter-to-external-tank attach-strut configurations. The entire model was supported by means of a balance mounted in the orbiter through its base and suspended from a sting.

  13. Aeroheating (pressure) characteristics on a 0.010-scale version of the vehicle 3 space shuttle configuration (26-OTS) in the Langley Research Center 4-foot wind tunnel (IH4), volume 1

    NASA Technical Reports Server (NTRS)

    Kingsland, R. B.

    1976-01-01

    The results of wind tunnel tests conducted on a 0.010-scale version of the Vehicle 3 Space Shuttle Configuration were presented. Pressure measurements were made on the launch configuration, orbiter alone, external tank alone, and solid rocket booster alone, to provide heat transfer pressure data. The tests were conducted for a Mach number range from 2.36 to 4.6 and Reynolds number range from 1.2 to 5 million per foot. The model was tested at angles of attack from -10 deg to 20 deg for a sideslip angle range from -5 deg to +5 deg and at sideslip angles from -5 deg to 48 deg for 0 deg angle of attack.

  14. Could oxytocin administration during labor contribute to autism and related behavioral disorders?--A look at the literature.

    PubMed

    Wahl, Roy U Rojas

    2004-01-01

    This literature review summarizes recent potential evidence, most of which is at the molecular/mechanistic level, in support of Hollander's hypothesis that excess oxytocin (OT), possibly through OT administration at birth, could contribute to the development of autistic spectrum disorders and related syndromes by proposed down regulation of the OT receptor (OTR). In this review, recent molecular evidence for OTR internalization by excess OT is related to OT's reported effects on animal social behavior, favoring social bondage, notably in sheep, voles, rats and especially mice. Adding indications for OT's capability of crossing the maternal placenta and OT's possibility of crossing an underdeveloped or stressed infantile blood brain barrier at birth, a causal connection between OT excess and behavioral disorders such as autism can be supported from a molecular perspective. Possible strategies such as a thorough statistical analysis of numerous birth records as well as molecular studies such as radiotracing using labeled OT are proposed to test this hypothesis. PMID:15288368

  15. Oxytocin and Social Adaptation: Insights from Neuroimaging Studies of Healthy and Clinical Populations.

    PubMed

    Ma, Yina; Shamay-Tsoory, Simone; Han, Shihui; Zink, Caroline F

    2016-02-01

    Adaptation to the social environment is critical for human survival. The neuropeptide oxytocin (OT), implicated in social cognition and emotions pivotal to sociality and well-being, is a promising pharmacological target for social and emotional dysfunction. We suggest here that the multifaceted role of OT in socio-affective processes improves the capability for social adaptation. We review OT effects on socio-affective processes, with a focus on OT-neuroimaging studies, to elucidate neuropsychological mechanisms through which OT promotes social adaptation. We also review OT-neuroimaging studies of individuals with social deficits and suggest that OT ameliorates impaired social adaptation by normalizing hyper- or hypo-brain activity. The social adaption model (SAM) provides an integrative understanding of discrepant OT effects and the modulations of OT action by personal milieu and context.

  16. Oxytocin and Social Adaptation: Insights from Neuroimaging Studies of Healthy and Clinical Populations.

    PubMed

    Ma, Yina; Shamay-Tsoory, Simone; Han, Shihui; Zink, Caroline F

    2016-02-01

    Adaptation to the social environment is critical for human survival. The neuropeptide oxytocin (OT), implicated in social cognition and emotions pivotal to sociality and well-being, is a promising pharmacological target for social and emotional dysfunction. We suggest here that the multifaceted role of OT in socio-affective processes improves the capability for social adaptation. We review OT effects on socio-affective processes, with a focus on OT-neuroimaging studies, to elucidate neuropsychological mechanisms through which OT promotes social adaptation. We also review OT-neuroimaging studies of individuals with social deficits and suggest that OT ameliorates impaired social adaptation by normalizing hyper- or hypo-brain activity. The social adaption model (SAM) provides an integrative understanding of discrepant OT effects and the modulations of OT action by personal milieu and context. PMID:26616296

  17. Effect of oxytocin injection to milching buffaloes on its content & stability in milk

    PubMed Central

    Pullakhandam, Raghu; Palika, Ravindranadh; Vemula, Sudershan Rao; Polasa, Kalpagam; Boindala, Sesikeran

    2014-01-01

    Background & objectives: Oxytocin (OT) injections to milch cattle for milk letdown have become a common practice amongst dairy farmers in India. Although there is no reported evidence, it is widely presumed that long term consumption of such milk leads to adverse health consequences. However, there is no information on the effect of exogenous OT injections on milk OT content and its stability during heating and gastrointestinal digestion. This study was carried out to determine the OT content in milk samples given by buffaloes with and without OT injections and to assess the stability of OT in the milk. Methods: Milk samples from milch buffaloes (Murrah buffalo) were collected from local farmers with (n=121) or without (n=120) exogenous OT injections during 3 to 5 months of lactation period. The OT content of milk samples was estimated by competitive enzyme immunoassay (EIA). The thermal and digestive stability of OT was assessed by in silico and in vitro digestion methods. Results: The OT content of the milk samples was similar regardless of OT injections used. Further, OT was found to be stable to heat treatment and gastric pepsin digestion while it was rapidly digested during the simulated intestinal digestion. Reduced OT was digested by pepsin, implying that internal disulphide bridge of OT rendered the peptide resistant to peptic digestion. On the other hand, phenylmethylsulphonyl fluoride (PMSF), a serine protease inhibitor, abrogated the pancreatin induced digestion of OT. Interpretation & conclusions: These findings suggest that exogenous OT injections do not influence its content in milk. Further, OT present in milk is rapidly degraded during intestinal digestion, ruling out its intestinal absorption and associated adverse health consequences, if any. PMID:25109729

  18. Does Oxytocin Increase Trust in Humans? A Critical Review of Research.

    PubMed

    Nave, Gideon; Camerer, Colin; McCullough, Michael

    2015-11-01

    Behavioral neuroscientists have shown that the neuropeptide oxytocin (OT) plays a key role in social attachment and affiliation in nonhuman mammals. Inspired by this initial research, many social scientists proceeded to examine the associations of OT with trust in humans over the past decade. To conduct this work, they have (a) examined the effects of exogenous OT increase caused by intranasal administration on trusting behavior, (b) correlated individual difference measures of OT plasma levels with measures of trust, and (c) searched for genetic polymorphisms of the OT receptor gene that might be associated with trust. We discuss the different methods used by OT behavioral researchers and review evidence that links OT to trust in humans. Unfortunately, the simplest promising finding associating intranasal OT with higher trust has not replicated well. Moreover, the plasma OT evidence is flawed by how OT is measured in peripheral bodily fluids. Finally, in recent large-sample studies, researchers failed to find consistent associations of specific OT-related genetic polymorphisms and trust. We conclude that the cumulative evidence does not provide robust convergent evidence that human trust is reliably associated with OT (or caused by it). We end with constructive ideas for improving the robustness and rigor of OT research. PMID:26581735

  19. Plasma Oxytocin in Children with Autism and Its Correlations with Behavioral Parameters in Children and Parents

    PubMed Central

    Husarova, Veronika Marcincakova; Lakatosova, Silvia; Pivovarciova, Anna; Babinska, Katarina; Bakos, Jan; Durdiakova, Jaroslava; Kubranska, Aneta; Ostatnikova, Daniela

    2016-01-01

    Objective Oxytocin (OT) has been implicated to play an important role in autism spectrum disorders (ASD) etiology. We aimed to find out the differences in plasma OT levels between children with autism and healthy children, the associations of OT levels with particular autism symptoms and the associations of particular parental autistic traits with their ASD children OT levels. Methods We included 19 boys with autism and 44 healthy age-matched boys. OT levels were analyzed by ELISA method. Children with autism were scored by Childhood Autism Rating Scale and Autism Diagnostic Interview (ADI), adjusted research version. Autism Spectrum Quotient (AQ), Systemizing Quotient (SQ) and Empathizing Quotient were completed by parents of children with autism. Results Children with autism had significantly lower plasma OT levels than controls. OT levels positively correlated with ADI Reciprocal Interaction and Communication scores. AQ and SQ of fathers positively correlated with children plasma OT level. Conclusion Our results support the hypothesis of OT deficiency in autism. The "paradoxical" associations of OT levels and social skills in children with autism indicate disturbances at various levels of OT system. We first reported associations of OT levels in children with autism and behavioral measures in fathers indicating that OT abnormalities stay between parental autistic traits and autism symptoms in their children. PMID:27081377

  20. Relationship of plasma oxytocin levels to baseline symptoms and symptom changes during three weeks of daily oxytocin administration in people with schizophrenia.

    PubMed

    Lee, Mary R; Wehring, Heidi J; McMahon, Robert P; Liu, Fang; Linthicum, Jared; Verbalis, Joseph G; Buchanan, Robert W; Strauss, Gregory P; Rubin, Leah H; Kelly, Deanna L

    2016-04-01

    Several clinical studies have found an inverse relationship between clinical symptoms and peripheral oxytocin (OT) levels in people with schizophrenia. As oxytocin is a putative treatment for schizophrenia, the effect of repeated dosing of OT on OT levels, clinical symptoms and the relationship between the two is of interest. In a, randomized, double blind, parallel group 3 week study (N=28) with daily administration of intranasal OT (20 IU twice daily) or placebo (PBO), we examined the effect of OT administration on the correlation between the change in peripheral OT levels and change in clinical symptoms in patients with schizophrenia. At baseline, there were no significant treatment group differences in OT levels. There were no significant associations between baseline OT levels and any symptom measures. After 3 weeks of OT/PBO dosing, there was no significant difference in the magnitude of change in OT levels between the two treatment groups. Correlations between changes in peripheral OT levels and changes in the BPRS total and negative symptom scores were not different between treatment groups. Larger studies are needed to examine the effect of exogenous OT on peripheral OT levels and the relationship between the latter and clinical symptoms. Clinical Trials.gov=NCT00884897. PMID:26879587

  1. Proteomics tools reveal startlingly high amounts of oxytocin in plasma and serum

    NASA Astrophysics Data System (ADS)

    Brandtzaeg, Ole Kristian; Johnsen, Elin; Roberg-Larsen, Hanne; Seip, Knut Fredrik; Maclean, Evan L.; Gesquiere, Laurence R.; Leknes, Siri; Lundanes, Elsa; Wilson, Steven Ray

    2016-08-01

    The neuropeptide oxytocin (OT) is associated with a plethora of social behaviors, and is a key topic at the intersection of psychology and biology. However, tools for measuring OT are still not fully developed. We describe a robust nano liquid chromatography-mass spectrometry (nanoLC-MS) platform for measuring the total amount of OT in human plasma/serum. OT binds strongly to plasma proteins, but a reduction/alkylation (R/A) procedure breaks this bond, enabling ample detection of total OT. The method (R/A + robust nanoLC-MS) was used to determine total OT plasma/serum levels to startlingly high concentrations (high pg/mL-ng/mL). Similar results were obtained when combining R/A and ELISA. Compared to measuring free OT, measuring total OT can have advantages in e.g. biomarker studies.

  2. 12 CFR 563b.660 - What must I include in my voluntary supervisory conversion application?

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... business plan that complies with § 563b.105, when required by OTS. (d) Financial data. (1) Your most recent... guidelines. See Thrift Activities Handbook Section 110 for guidelines at OTS's website...

  3. 76 FR 29334 - Iroquois FS&LA, Watseka, IL; Approval of Conversion Application

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-20

    ... are available for inspection by appointment (phone number: (202) 906-5922 or e-mail: public.info @ots.treas.gov"> public.info @ots.treas.gov ) at the Public Reading Room, 1700 G Street, NW., Washington,...

  4. 75 FR 24774 - Fairmount Bank, Baltimore, MD; Approval of Conversion Application

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-05

    ... are available for inspection by appointment (phone number: (202) 906-5922 or e-mail: public.info @ots.treas.gov"> public.info @ots.treas.gov ) at the Public Reading Room, 1700 G Street, NW., Washington,...

  5. 75 FR 51167 - Bank of Ruston, Ruston, Louisiana; Approval of Conversion Application

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-18

    ... application are available for inspection by appointment (phone number: (202) 906-5922 or e-mail: public.info @ots.treas.gov"> public.info @ots.treas.gov ) at the Public Reading Room, 1700 G Street,...

  6. 75 FR 52065 - SharePlus Federal Bank, Plano, TX; Approval of Conversion Application

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-24

    ... application are available for inspection by appointment (phone number: (202) 906-5922 or e-mail: public.info @ots.treas.gov"> public.info @ots.treas.gov ) at the Public Reading Room, 1700 G Street,...

  7. 76 FR 42768 - Capital Distribution

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-19

    ... Office of Thrift Supervision Capital Distribution AGENCY: Office of Thrift Supervision (OTS), Treasury... concerning the following information collection. Title of Proposal: Capital Distribution. OMB Number: 1550..., the information provides the OTS with a mechanism for monitoring capital distributions since...

  8. Proteomics tools reveal startlingly high amounts of oxytocin in plasma and serum

    PubMed Central

    Brandtzaeg, Ole Kristian; Johnsen, Elin; Roberg-Larsen, Hanne; Seip, Knut Fredrik; MacLean, Evan L.; Gesquiere, Laurence R.; Leknes, Siri; Lundanes, Elsa; Wilson, Steven Ray

    2016-01-01

    The neuropeptide oxytocin (OT) is associated with a plethora of social behaviors, and is a key topic at the intersection of psychology and biology. However, tools for measuring OT are still not fully developed. We describe a robust nano liquid chromatography-mass spectrometry (nanoLC-MS) platform for measuring the total amount of OT in human plasma/serum. OT binds strongly to plasma proteins, but a reduction/alkylation (R/A) procedure breaks this bond, enabling ample detection of total OT. The method (R/A + robust nanoLC-MS) was used to determine total OT plasma/serum levels to startlingly high concentrations (high pg/mL-ng/mL). Similar results were obtained when combining R/A and ELISA. Compared to measuring free OT, measuring total OT can have advantages in e.g. biomarker studies. PMID:27528413

  9. Proteomics tools reveal startlingly high amounts of oxytocin in plasma and serum.

    PubMed

    Brandtzaeg, Ole Kristian; Johnsen, Elin; Roberg-Larsen, Hanne; Seip, Knut Fredrik; MacLean, Evan L; Gesquiere, Laurence R; Leknes, Siri; Lundanes, Elsa; Wilson, Steven Ray

    2016-01-01

    The neuropeptide oxytocin (OT) is associated with a plethora of social behaviors, and is a key topic at the intersection of psychology and biology. However, tools for measuring OT are still not fully developed. We describe a robust nano liquid chromatography-mass spectrometry (nanoLC-MS) platform for measuring the total amount of OT in human plasma/serum. OT binds strongly to plasma proteins, but a reduction/alkylation (R/A) procedure breaks this bond, enabling ample detection of total OT. The method (R/A + robust nanoLC-MS) was used to determine total OT plasma/serum levels to startlingly high concentrations (high pg/mL-ng/mL). Similar results were obtained when combining R/A and ELISA. Compared to measuring free OT, measuring total OT can have advantages in e.g. biomarker studies. PMID:27528413

  10. Predicting the Migration Rate of Dialkyl Organotins from PVC Pipe into Water

    EPA Science Inventory

    Organotins (OTs) are additives widely used as thermal and light stabilizers in polyvinyl chloride (PVC) plastics. OTs can leach into water flowing through PVC pipes. This work examines the leaching rates of two neurotoxic OTs, dimethyl tin (DMT) and dibutyl tin (DBT), from PVC pi...

  11. 75 FR 41573 - Proposed Agency Information Collection Renewal; Joint Submission for OMB Review; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-16

    ... change to the OTS Internet Site at http://www.ots.treas.gov/pagehtml.cfm?catNumber=67&an=1 , including... comments received, go to http://www.ots.treas.gov/pagehtml.cfm?catNumber=67&an=1 . In addition, you...

  12. Maternal and Paternal Plasma, Salivary, and Urinary Oxytocin and Parent-Infant Synchrony: Considering Stress and Affiliation Components of Human Bonding

    ERIC Educational Resources Information Center

    Feldman, Ruth; Gordon, Ilanit; Zagoory-Sharon, Orna

    2011-01-01

    Studies in mammals have implicated the neuropeptide oxytocin (OT) in processes of bond formation and stress modulation, yet the involvement of OT in human bonding throughout life remains poorly understood. We assessed OT in the plasma, saliva, and urine of 112 mothers and fathers interacting with their 4-6-month-old infants. Parent-infant…

  13. Oxytocin reduces separation distress in piglets when given intranasally

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Oxytocin (OT) is one of the neurobiological foundations of sociality. It acts as a neuropeptide in numerous social processes, from ultra-social to anti-social behaviors. Evidence supports a role for OT in social support, possibly by attenuating separation distress. Nonetheless, research on OT is lac...

  14. The Effect of Previous Software Development Experience on Understanding the Object-Oriented Paradigm.

    ERIC Educational Resources Information Center

    Griffyth, Jacqui; Sharp, Helen

    1999-01-01

    Discusses the results of a survey to gain insight into the relationship between the acquisition of Object Technology (OT) concepts and previous software development experience. Indicates that the fundamental concepts of OT and the management of OT projects are not difficult to understand while analysis and design topics are harder. (Contains 18…

  15. Occupational Therapy: Roles and Functions in British Columbia Public Schools.

    ERIC Educational Resources Information Center

    Daniels, Linda E.; Backman, Catherine L.

    1992-01-01

    A survey of 73 school districts in British Columbia (Canada) evaluated availability of occupational therapy (OT) services, the most important aspects of OT evaluation, OT treatment and services in general, additional training needed by occupational therapists, demographic information on therapists, and therapists' current roles and functions. (DB)

  16. Job Choice and Personality: A Profile of Michigan Occupational and Physical Therapists.

    ERIC Educational Resources Information Center

    Lysack, Catherine; McNevin, Nancy; Dunleavy, Kim

    2001-01-01

    A Keirsey-Bates personality inventory was completed by 128 occupational therapists (OTs) and 166 physical therapists (PTs). PTs were far more likely to have sensing-judging temperament; OTs were predominantly sensing-perceiving or intuitive-feeling. The five most common OT types accounted for only 19% of PTs and the five most common PT types for…

  17. 76 FR 42767 - Management Officials Interlocks

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-19

    ... Office of Thrift Supervision Management Officials Interlocks AGENCY: Office of Thrift Supervision (OTS... Management and Budget (OMB) for review, as required by the Paperwork Reduction Act. Today, OTS is soliciting...: Management Officials Interlocks. OMB Number: 1550-0051. Form Number: N/A. Description: OTS uses the...

  18. 32 CFR 3.9 - Follow-on production contracts.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... condition set forth in law that requires non-Federal parties to the OT agreement to provide at least one... to the awardee of the OT prototype agreement for a specific number of units at specific target prices... the prototype project: (1) Ensure non-Federal parties to the OT prototype agreement offer at least...

  19. Learning Style of Students and Practitioners in Five Health Professions.

    ERIC Educational Resources Information Center

    Katz, Noomi; Heimann, Nanci

    1991-01-01

    An Israeli sample of 378 students and 251 practitioners in occupational therapy (OT), social work, nursing, physical therapy, and clinical psychology completed Kolb's Learning Style Inventory. Findings suggest greater variance in learning style among students. OT students were least abstract. Both OT students and practitioners were predominantly…

  20. Roles of oxytocin in spatial learning and memory in the nucleus basalis of Meynert in rats.

    PubMed

    Wu, Wei; Yu, Long-Chuan

    2004-08-15

    The present study was performed to explore the role of oxytocin (OT) in spatial learning and memory in the nucleus basalis of Meynert (NBM) of rats. The latency, distance and swimming path to find the platform were tested by Morris water maze and recorded by a video camera connected to a computer. Intra-NBM injections of 2 or 10 nmol of OT, but not 0.2 nmol of OT, induced significant increase on the latency of spatial learning. Rats receiving intra-NBM administrations of 2 or 10 nmol of OT showed a more random search pattern. There were no significant changes in the swimming speed in Morris water maze test after the injection of OT. Furthermore, the impaired effect of OT on the latency of spatial learning was blocked by intra-NBM injection of the selective OT antagonist Atosiban, indicating that the effect of OT was mediated by OT receptor in the NBM of rats. Moreover, there were no influences of OT or Atosiban on the retention performance in rats. The results suggest that OT plays an inhibitory role in spatial learning in the NBM; the effect is mediated by OT receptor.

  1. Ontogenesis of oxytocin pathways in the mammalian brain: late maturation and psychosocial disorders

    PubMed Central

    Grinevich, Valery; Desarménien, Michel G.; Chini, Bice; Tauber, Maithé; Muscatelli, Françoise

    2014-01-01

    Oxytocin (OT), the main neuropeptide of sociality, is expressed in neurons exclusively localized in the hypothalamus. During the last decade, a plethora of neuroendocrine, metabolic, autonomic and behavioral effects of OT has been reported. In the urgency to find treatments to syndromes as invalidating as autism, many clinical trials have been launched in which OT is administered to patients, including adolescents and children. However, the impact of OT on the developing brain and in particular on the embryonic and early postnatal maturation of OT neurons, has been only poorly investigated. In the present review we summarize available (although limited) literature on general features of ontogenetic transformation of the OT system, including determination, migration and differentiation of OT neurons. Next, we discuss trajectories of OT receptors (OTR) in the perinatal period. Furthermore, we provide evidence that early alterations, from birth, in the central OT system lead to severe neurodevelopmental diseases such as feeding deficit in infancy and severe defects in social behavior in adulthood, as described in Prader-Willi syndrome (PWS). Our review intends to propose a hypothesis about developmental dynamics of central OT pathways, which are essential for survival right after birth and for the acquisition of social skills later on. A better understanding of the embryonic and early postnatal maturation of the OT system may lead to better OT-based treatments in PWS or autism. PMID:25767437

  2. Anatomy in Occupational Therapy Program Curriculum: Practitioners' Perspectives

    ERIC Educational Resources Information Center

    Schofield, Katherine Anne

    2014-01-01

    Anatomy education is undergoing significant transformation. It is unknown whether changes are in accordance with occupational therapy (OT) practice needs. The purpose of this pilot study was to survey OT clinicians to determine their perspectives on the value of anatomy in OT curricula, and anatomical knowledge required for practice. In addition…

  3. 75 FR 82217 - Community Reinvestment Act Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-30

    ... for CRA purposes by the OCC, Board, and FDIC on August 2, 2005, effective September 1, 2005. 70 FR... 22, 2007, effective July 1, 2007. 72 FR 13429. As OTS explained in the preamble, OTS decided to index... FR at 13432. OTS also noted that the particular adjustment formula adopted is also used under...

  4. Septal oxytocin administration impairs peer affiliation via V1a receptors in female meadow voles.

    PubMed

    Anacker, Allison M J; Christensen, Jennifer D; LaFlamme, Elyssa M; Grunberg, Diana M; Beery, Annaliese K

    2016-06-01

    The peptide hormone oxytocin (OT) plays an important role in social behaviors, including social bond formation. In different contexts, however, OT is also associated with aggression, social selectivity, and reduced affiliation. Female meadow voles form social preferences for familiar same-sex peers under short, winter-like day lengths in the laboratory, and provide a means of studying affiliation outside the context of reproductive pair bonds. Multiple lines of evidence suggest that the actions of OT in the lateral septum (LS) may decrease affiliative behavior, including greater density of OT receptors in the LS of meadow voles that huddle less. We infused OT into the LS of female meadow voles immediately prior to cohabitation with a social partner to determine its effects on partner preference formation. OT prevented the formation of preferences for the partner female. Co-administration of OT with a specific OT receptor antagonist did not reverse the effect, but co-administration of OT with a specific vasopressin 1a receptor (V1aR) antagonist did, indicating that OT in the LS likely acted through V1aRs to decrease partner preference. Receptor autoradiography revealed dense V1aR binding in the LS of female meadow voles. These results suggest that the LS is a brain region that may be responsible for inhibitory effects of OT administration on affiliation, which will be important to consider in therapeutic administrations of OT. PMID:26974500

  5. 76 FR 44656 - Privacy Act of 1974, as Amended

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-26

    ... Supervision (OTS). A list of the systems of the OTS can be found at 74 FR 31103, June 29, 2009, and can be updated with reference to 76 FR 7243 and 76 FR 7242, February 9, 2011. DATES: Effective Date: July 21...), the OTS will be abolished. On July 21, 2011 (the designated transfer date under section 1062 of...

  6. The role of oxytocin in cardiovascular regulation

    PubMed Central

    Gutkowska, J.; Jankowski, M.; Antunes-Rodrigues, J.

    2014-01-01

    Studies of body volume expansion have indicated that lesions of the anteroventral third ventricle and median eminence block the release of atrial natriuretic peptide (ANP) into the circulation. Detailed analysis of the lesions showed that activation of oxytocin (OT)-ergic neurons is responsible for ANP release, and it has become clear that activation of neuronal circuitry elicits OT secretion into the circulation, activating atrial OT receptors and ANP release from the heart. Subsequently, we have uncovered the entire functional OT system in the rat and the human heart. An abundance of OT has been observed in the early development of the fetal heart, and the capacity of OT to generate cardiomyocytes (CMs) has been demonstrated in various types of stem cells. OT treatment of mesenchymal stem cells stimulates paracrine factors beneficial for cardioprotection. Cardiovascular actions of OT include: i) lowering blood pressure, ii) negative inotropic and chronotropic effects, iii) parasympathetic neuromodulation, iv) vasodilatation, v) anti-inflammatory activity, vi) antioxidant activity, and vii) metabolic effects. OT actions are mediated by nitric oxide and ANP. The beneficial actions of OT may include the increase in glucose uptake by CMs and stem cells, reduction in CM hypertrophy, oxidative stress, and mitochondrial protection of several cell types. In experimentally induced myocardial infarction in rats, continuous in vivo OT delivery improves cardiac healing and cardiac work, reduces inflammation, and stimulates angiogenesis. Because OT plays anti-inflammatory and cardioprotective roles and improves vascular and metabolic functions, it demonstrates potential for therapeutic use in various pathologic conditions. PMID:24676493

  7. Central oxytocin receptor stimulation attenuates the orexigenic effects of butorphanol tartrate.

    PubMed

    Olszewski, Pawel K; Klockars, Oscar A; Klockars, Anica; Levine, Allen S

    2016-09-28

    Butorphanol tartrate (BT), a mixed µ/κ/δ opioid receptor agonist, is one of the most potent orexigens known to date. The central mechanisms through which BT causes hyperphagia are largely unknown. Interestingly, BT suppresses meal-end activation of neurons synthesizing anorexigenic neuropeptide, oxytocin (OT), which suggests that BT promotes hyperphagia by silencing OT-derived satiety signaling. As OT terminates consumption by acting by distinct hindbrain and forebrain circuits, we investigated whether stimulation of the OT receptor in the forebrain or the hindbrain [through lateral ventricular (LV) and fourth ventricular (4V) OT injections] leads to termination of food intake induced by BT. We established effective doses of BT on chow intake in ad-libitum-fed and overnight-deprived rats as well as effective doses of LV and 4V OT in deprived animals. Then, we determined doses of LV and 4V OT that reduce hyperphagia produced by BT in sated and deprived rats. Finally, we assessed whether OT's effects on BT-induced feeding can be suppressed by an OT receptor antagonist. 4 mg/kg BT increased intake in ad-libitum-fed and overnight-deprived rats, whereas LV and 4V OT at 1 μg caused a decrease in deprived rats. BT-induced chow intake in hungry and sated animals was suppressed by a very low, 0.1 μg dose of 4V OT, whereas 1 μg OT was effective LV. The effect of OT was attenuated by OT receptor antagonist, L-368 899. Reduced activity of the OT circuit, especially its hindbrain component, is a critical factor in shaping the magnitude of consumption in response to BT treatment. PMID:27471903

  8. Oxytocin facilitates fidelity in well-established marmoset pairs by reducing sociosexual behavior toward opposite-sex strangers.

    PubMed

    Cavanaugh, Jon; Mustoe, Aaryn C; Taylor, Jack H; French, Jeffrey A

    2014-11-01

    Behavioral strategies that facilitate the maintenance of social bonds are critical for the preservation of high-quality social relationships. Central oxytocin (OT) activity modulates the behavioral features of socially monogamous relationships in a number of mammalian species (including marmoset monkeys), and plays a vital role in the behavioral maintenance of long-term social relationships. Two distinct variants of OT have been identified in some New World primates (including marmosets; Lee et al., 2011). The marmoset variant of the oxytocin ligand (Pro(8)-OT) is structurally distinct from the consensus mammalian variant of the oxytocin ligand (Leu(8)-OT), due to a proline substitution at the 8th amino-acid position. The goal of the present study was to determine if treating marmosets with Pro(8)-OT, relative to treatments with Leu(8)-OT, control saline, or an OT antagonist, had modulatory effects on the behavioral maintenance of long-term social relationships in marmosets. Treatment with the Pro(8) variant, but not the Leu(8) variant, of OT facilitated fidelity with a long-term partner by reducing time spent in close proximity with an opposite-sex stranger. However, this facilitative effect of Pro(8)-OT on proximity behavior manifested itself differently in male and female marmosets, such that females preferred to interact socially with their partner rather than a stranger when treated with Pro(8)-OT, while males spent less time in close proximity with both their partner and a stranger when treated with Pro(8)-OT. Furthermore, treatment with Pro(8)-OT, but not Leu(8)-OT, significantly delayed the expression of sexual solicitation behavior toward an opposite-sex stranger in both male and female marmosets, but had no effect on sociosexual behavior directed toward a long-term partner. These results suggest that the OT system is highly involved in reducing fidelity-threatening behaviors in well-established marmoset pairs, and that the effects were only produced by

  9. An investigation of plasma and salivary oxytocin responses in breast- and formula-feeding mothers of infants

    PubMed Central

    GREWEN, KAREN M.; DAVENPORT, RUSSELL E.; LIGHT, KATHLEEN C.

    2011-01-01

    Oxytocin (OT) is a peptide increasingly studied in relation to human social interactions, affiliation, and clinical disorders. Studies are constrained by use of invasive blood draws and would benefit from a reliable salivary OT assay. Our goals were to examine feasibility of salivary OT measurement, compare salivary to plasma OT responses in 12 breast- and 8 formula-feeding mothers, and assess the degree of correlation between plasma and salivary OT. Using a commercial EIA kit, we measured OT in 5 saliva and 7 plasma samples in a protocol designed to elicit changes in OT (Rest, Infant Interaction, Stress, Feeding). Breast-feeders had higher OT levels than formula-feeders across all conditions in plasma (+36%) and saliva (+23%). OT levels and ranges were similar in saliva and plasma, with slightly greater variance in saliva. Concurrently sampled plasma and salivary OT were correlated at end of Baseline Rest (r = +.59, p = .022) and Post-Stress Recovery (r = +.59, p = .025). These data suggest that salivary OT assay is feasible, and will be of value where plasma samples are not possible. Validation with larger samples is needed. PMID:20102537

  10. Salivary Oxytocin Concentrations in Males following Intranasal Administration of Oxytocin: A Double-Blind, Cross-Over Study.

    PubMed

    Daughters, Katie; Manstead, Antony S R; Hubble, Kelly; Rees, Aled; Thapar, Anita; van Goozen, Stephanie H M

    2015-01-01

    The use of intranasal oxytocin (OT) in research has become increasingly important over the past decade. Although researchers have acknowledged a need for further investigation of the physiological effects of intranasal administration, few studies have actually done so. In the present double-blind cross-over study we investigated the longevity of a single 24 IU dose of intranasal OT measured in saliva in 40 healthy adult males. Salivary OT concentrations were significantly higher in the OT condition, compared to placebo. This significant difference lasted until the end of testing, approximately 108 minutes after administration, and peaked at 30 minutes. Results showed significant individual differences in response to intranasal OT administration. To our knowledge this is the largest and first all-male within-subjects design study to demonstrate the impact of intranasal OT on salivary OT concentrations. The results are consistent with previous research in suggesting that salivary OT is a valid matrix for OT measurement. The results also suggest that the post-administration 'wait-time' prior to starting experimental tasks could be reduced to 30 minutes, from the 45 minutes typically used, thereby enabling testing during peak OT concentrations. Further research is needed to ascertain whether OT concentrations after intranasal administration follow similar patterns in females, and different age groups. PMID:26669935

  11. SCIRehab Project Series: The Occupational Therapy Taxonomy

    PubMed Central

    Ozelie, Rebecca; Sipple, Catherine; Foy, Teresa; Cantoni, Kara; Kellogg, Katherine; Lookingbill, Jennifer; Backus, Deborah; Gassaway, Julie

    2009-01-01

    Background/Objective: Lack of a classification system for occupational therapy (OT) rehabilitation interventions for traumatic spinal cord injury (SCI) rehabilitation in the United States makes conducting outcomes research difficult. This article describes an OT SCI rehabilitation taxonomy (system to categorize and classify treatments). Methods: OT clinicians and researchers from 6 SCI rehabilitation centers developed a taxonomy to describe details of each OT session. This effort is part of the SCIRehab study, which uses the practice-based evidence, observational research methodology to examine current treatment processes without changing existing practice. Results: The OT taxonomy consists of 26 OT activities (eg, training on activities of daily living, communication, home management skills, wheelchair mobility, bed mobility, transfers, balance, strengthening, stretching, equipment evaluation, and community reintegration). Time spent on each activity is documented along with therapeutic interventions used to facilitate the activity. Treatment descriptions are enhanced further with identification of assistance needs, patient direction of care, and family involvement, which help to describe and guide OT activity selection. The OT taxonomy documentation process includes all OT rehabilitation interventions for patients with SCI while maintaining efficiency in data collection. Conclusion: The electronic documentation system is being used at 6 centers for all OT sessions with 1,500 patients with acute traumatic SCI. It is the largest known attempt to document details of the comprehensive OT rehabilitation process for patients with SCI in the United States. PMID:19810630

  12. Oxytocin and cardioprotection in diabetes and obesity.

    PubMed

    Jankowski, Marek; Broderick, Tom L; Gutkowska, Jolanta

    2016-01-01

    Oxytocin (OT) emerges as a drug for the treatment of diabetes and obesity. The entire OT system is synthesized in the rat and human heart. The direct myocardial infusion with OT into an ischemic or failing heart has the potential to elicit a variety of cardioprotective effects. OT treatment attenuates cardiomyocyte (CMs) death induced by ischemia-reperfusion by activating pro-survival pathways within injured CMs in vivo and in isolated cells. OT treatment reduces cardiac apoptosis, fibrosis, and hypertrophy. The OT/OT receptor (OTR) system is downregulated in the db/db mouse model of type 2 diabetes which develops genetic diabetic cardiomyopathy (DC) similar to human disease. We have shown that chronic OT treatment prevents the development of DC in the db/db mouse. In addition, OT stimulates glucose uptake in both cardiac stem cells and CMs, and increases cell resistance to diabetic conditions. OT may help replace lost CMs by stimulating the in situ differentiation of cardiac stem cells into functional mature CMs. Lastly, adult stem cells amenable for transplantation such as MSCs could be preconditioned with OT ex vivo and implanted into the injured heart to aid in tissue regeneration through direct differentiation, secretion of protective and cardiomyogenic factors and/or their fusion with injured CMs. PMID:27268060

  13. Stability of small ubiquitin-like modifier (SUMO) proteases OVERLY TOLERANT TO SALT1 and -2 modulates salicylic acid signalling and SUMO1/2 conjugation in Arabidopsis thaliana

    PubMed Central

    Bailey, Mark; Srivastava, Anjil; Conti, Lucio; Nelis, Stuart; Zhang, Cunjin; Florance, Hannah; Love, Andrew; Milner, Joel; Napier, Richard; Grant, Murray; Sadanandom, Ari

    2016-01-01

    Small ubiquitin-like modifier proteases 1 and 2 (SUMO1/2) have been linked to the regulation of salicylic acid (SA)-mediated defence signalling in Arabidopsis thaliana. In order to define the role of the SUMO proteases OVERLY TOLERANT TO SALT1 and -2 (OTS1/2) in defence and to provide insight into SUMO1/2-mediated regulation of SA signalling, we examined the status of SA-mediated defences in ots1/2 mutants. The ots1 ots2 double mutant displayed enhanced resistance to virulent Pseudomonas syringae and higher levels of SA compared with wild-type (WT) plants. Furthermore, ots1 ots2 mutants exhibited upregulated expression of the SA biosynthesis gene ICS1 in addition to enhanced SA-responsive ICS1 expression beyond that of WT. SA stimulated OTS1/2 degradation and promoted accumulation of SUMO1/2 conjugates. These results indicate that OTS1 and -2 act in a feedback loop in SA signalling and that de novo OTS1/2 synthesis works antagonistically to SA-promoted degradation, adjusting the abundance of OTS1/2 to moderate SA signalling. Accumulation of SUMO1/2 conjugates coincides with SA-promoted OTS degradation and may play a positive role in SA-mediated signalling in addition to its repressive roles reported elsewhere. PMID:26494731

  14. Regulation of 5alpha-reductase isoforms by oxytocin in the rat ventral prostate.

    PubMed

    Assinder, S J; Johnson, C; King, K; Nicholson, H D

    2004-12-01

    Oxytocin (OT) is present in the male reproductive tract, where it is known to modulate contractility, cell growth, and steroidogenesis. Little is known about how OT regulates these processes. This study describes the localization of OT receptor in the rat ventral prostate and investigates if OT regulates gene expression and/or activity of 5alpha-reductase isoforms I and II. The ventral prostates of adult male Wistar rats were collected following daily sc administration of saline (control), OT, a specific OT antagonist or both OT plus antagonist for 3 d. Expression of the OT receptor was identified in the ventral prostate by RT-PCR and Western blot, and confirmed to be a single active binding site by radioreceptor assay. Immunohistochemistry localized the receptor to the epithelium of prostatic acini and to the stromal tissue. Real-time RT-PCR determined that OT treatment significantly reduced expression of 5alpha-reductase I but significantly increased 5alpha-reductase II expression in the ventral prostate. Activity of both isoforms of 5alpha-reductase was significantly increased by OT, resulting in increased concentration of prostatic dihydrotestosterone. In conclusion, OT is involved in regulating conversion of testosterone to the biologically active dihydrotestosterone in the rat ventral prostate. It does so by differential regulation of 5alpha-reductase isoforms I and II.

  15. Stability of small ubiquitin-like modifier (SUMO) proteases OVERLY TOLERANT TO SALT1 and -2 modulates salicylic acid signalling and SUMO1/2 conjugation in Arabidopsis thaliana.

    PubMed

    Bailey, Mark; Srivastava, Anjil; Conti, Lucio; Nelis, Stuart; Zhang, Cunjin; Florance, Hannah; Love, Andrew; Milner, Joel; Napier, Richard; Grant, Murray; Sadanandom, Ari

    2016-01-01

    Small ubiquitin-like modifier proteases 1 and 2 (SUMO1/2) have been linked to the regulation of salicylic acid (SA)-mediated defence signalling in Arabidopsis thaliana. In order to define the role of the SUMO proteases OVERLY TOLERANT TO SALT1 and -2 (OTS1/2) in defence and to provide insight into SUMO1/2-mediated regulation of SA signalling, we examined the status of SA-mediated defences in ots1/2 mutants. The ots1 ots2 double mutant displayed enhanced resistance to virulent Pseudomonas syringae and higher levels of SA compared with wild-type (WT) plants. Furthermore, ots1 ots2 mutants exhibited upregulated expression of the SA biosynthesis gene ICS1 in addition to enhanced SA-responsive ICS1 expression beyond that of WT. SA stimulated OTS1/2 degradation and promoted accumulation of SUMO1/2 conjugates. These results indicate that OTS1 and -2 act in a feedback loop in SA signalling and that de novo OTS1/2 synthesis works antagonistically to SA-promoted degradation, adjusting the abundance of OTS1/2 to moderate SA signalling. Accumulation of SUMO1/2 conjugates coincides with SA-promoted OTS degradation and may play a positive role in SA-mediated signalling in addition to its repressive roles reported elsewhere.

  16. Salivary Oxytocin Concentrations in Males following Intranasal Administration of Oxytocin: A Double-Blind, Cross-Over Study

    PubMed Central

    Daughters, Katie; Manstead, Antony S. R.; Hubble, Kelly; Rees, Aled; Thapar, Anita; van Goozen, Stephanie H. M.

    2015-01-01

    The use of intranasal oxytocin (OT) in research has become increasingly important over the past decade. Although researchers have acknowledged a need for further investigation of the physiological effects of intranasal administration, few studies have actually done so. In the present double-blind cross-over study we investigated the longevity of a single 24 IU dose of intranasal OT measured in saliva in 40 healthy adult males. Salivary OT concentrations were significantly higher in the OT condition, compared to placebo. This significant difference lasted until the end of testing, approximately 108 minutes after administration, and peaked at 30 minutes. Results showed significant individual differences in response to intranasal OT administration. To our knowledge this is the largest and first all-male within-subjects design study to demonstrate the impact of intranasal OT on salivary OT concentrations. The results are consistent with previous research in suggesting that salivary OT is a valid matrix for OT measurement. The results also suggest that the post-administration ‘wait-time’ prior to starting experimental tasks could be reduced to 30 minutes, from the 45 minutes typically used, thereby enabling testing during peak OT concentrations. Further research is needed to ascertain whether OT concentrations after intranasal administration follow similar patterns in females, and different age groups. PMID:26669935

  17. Oxytocin and cardioprotection in diabetes and obesity.

    PubMed

    Jankowski, Marek; Broderick, Tom L; Gutkowska, Jolanta

    2016-06-07

    Oxytocin (OT) emerges as a drug for the treatment of diabetes and obesity. The entire OT system is synthesized in the rat and human heart. The direct myocardial infusion with OT into an ischemic or failing heart has the potential to elicit a variety of cardioprotective effects. OT treatment attenuates cardiomyocyte (CMs) death induced by ischemia-reperfusion by activating pro-survival pathways within injured CMs in vivo and in isolated cells. OT treatment reduces cardiac apoptosis, fibrosis, and hypertrophy. The OT/OT receptor (OTR) system is downregulated in the db/db mouse model of type 2 diabetes which develops genetic diabetic cardiomyopathy (DC) similar to human disease. We have shown that chronic OT treatment prevents the development of DC in the db/db mouse. In addition, OT stimulates glucose uptake in both cardiac stem cells and CMs, and increases cell resistance to diabetic conditions. OT may help replace lost CMs by stimulating the in situ differentiation of cardiac stem cells into functional mature CMs. Lastly, adult stem cells amenable for transplantation such as MSCs could be preconditioned with OT ex vivo and implanted into the injured heart to aid in tissue regeneration through direct differentiation, secretion of protective and cardiomyogenic factors and/or their fusion with injured CMs.

  18. CSF and blood oxytocin concentration changes following intranasal delivery in macaque.

    PubMed

    Dal Monte, Olga; Noble, Pamela L; Turchi, Janita; Cummins, Alex; Averbeck, Bruno B

    2014-01-01

    Oxytocin (OT) in the central nervous system (CNS) influences social cognition and behavior, making it a candidate for treating clinical disorders such as schizophrenia and autism. Intranasal administration has been proposed as a possible route of delivery to the CNS for molecules like OT. While intranasal administration of OT influences social cognition and behavior, it is not well established whether this is an effective means for delivering OT to CNS targets. We administered OT or its vehicle (saline) to 15 primates (Macaca mulatta), using either intranasal spray or a nebulizer, and measured OT concentration changes in the cerebral spinal fluid (CSF) and in blood. All subjects received both delivery methods and both drug conditions. Baseline samples of blood and CSF were taken immediately before drug administration. Blood was collected every 10 minutes after administration for 40 minutes and CSF was collected once post-delivery, at the 40 minutes time point. We found that intranasal administration of exogenous OT increased concentrations in both CSF and plasma compared to saline. Both delivery methods resulted in similar elevations of OT concentration in CSF, while the changes in plasma OT concentration were greater after nasal spray compared to nebulizer. In conclusion our study provides evidence that both nebulizer and nasal spray OT administration can elevate CSF OT levels. PMID:25133536

  19. Oxytocin's role in anxiety: a critical appraisal.

    PubMed

    MacDonald, Kai; Feifel, David

    2014-09-11

    A growing literature suggests that the oxytocin (OT) system may play a role in human anxiety states, anxiety-related traits, and moreover, that this system may be a target for the development of novel anxiolytic treatments. However, studies of OT's acute and chronic effects on various aspects of anxiety have produced mixed results. In this forward-looking review, we discuss the myriad phenomena to which the term "anxiety" is applied in the OT literature and the problem this presents developing a coherent picture of OT's role in anxiety. We then survey several different fields of research that support the role of the OT system in human anxiety, including evolutionary perspectives, translational and neuroimaging research, genetic studies, and clinical trials of intranasal OT. As an outgrowth of this data, we propose a "bowtie" model of OT's role at the interface of social attachment and anxiety. We next direct attention to understudied brain regions and neural circuits which may be important to study in OT experiments in humans anxiety disorders. Finally, we conclude by proposing questions and priorities for studying both the clinical potential of OT in anxiety, as well as mechanisms that may underlie this potential. Crucially, these priorities include targeted proof-of-concept clinical trials of IN OT in certain anxiety disorders, including investigations of individual moderators of OT's anxiolytic effects (i.e. sex, genetic factors, and early experience). This article is part of a Special Issue entitled Oxytocin and Social Behav.

  20. Central oxytocin regulates social familiarity and scent marking behavior that involves amicable odor signals between male mice.

    PubMed

    Arakawa, Hiroyuki; Blanchard, D Caroline; Blanchard, Robert J

    2015-07-01

    The effect of oxytocin on social behavior and odor communication was investigated in male C57BL/6J mice. In three-male colonies, in visible burrow systems, icv oxytocin (OT) infusion before colony formation substantially increased huddling together over the initial 8 h of grouping, accompanied by decreased expression of a number of social approaches associated with conspecific aggression and defense. OT antagonist infusion had little impact on expression of social approaches but decreased time engaging in social components including huddle over the initial 8 h. These results demonstrate a linkage of social familiarity to OT availability in the brain. In a scent marking paradigm central infusion of OT reduced territorial marking towards male conspecifics, and this in turn reduced the scent marking of untreated stimulus males to OT-infused subjects. Infusion of an OT antagonist into stimulus mice who were confronted with OT-infused subjects prevented the reduction/suppression of scent marking that was normally seen following exposure of social odors released from OT-injected mice. Odor of pair-housed mice also induced a suppression of territorial scent marking in odor recipients, but OT antagonist administration into pair-housed mice blocked this suppressive effect of odor cue. These results indicate that central OT modulates release as well as detection of amicable signals facilitating/maintaining familiar relationships and suppressing territorial behavior between male mice. Overall, these findings suggest that OT plays a significant role in regulating social familiarity via changing qualities of conspecific odor cues.

  1. Central Oxytocin and Food Intake: Focus on Macronutrient-Driven Reward

    PubMed Central

    Klockars, Anica; Levine, Allen Stuart; Olszewski, Pawel Karol

    2015-01-01

    Centrally acting oxytocin (OT) is known to terminate food consumption in response to excessive stomach distension, increase in salt loading, and presence of toxins. Hypothalamic-hindbrain OT pathways facilitate these aspects of OT-induced hypophagia. However, recent discoveries have implicated OT in modifications of feeding via reward circuits: OT has been found to differentially affect consumption of individual macronutrients in choice and no-choice paradigms. In this mini-review, we focus on presenting and interpreting evidence that defines OT as a key component of mechanisms that reduce eating for pleasure and shape macronutrient preferences. We also provide remarks on challenges in integrating the knowledge on physiological and pathophysiological states in which both OT activity and macronutrient preferences are affected. PMID:25972841

  2. Central oxytocin and food intake: focus on macronutrient-driven reward.

    PubMed

    Klockars, Anica; Levine, Allen Stuart; Olszewski, Pawel Karol

    2015-01-01

    Centrally acting oxytocin (OT) is known to terminate food consumption in response to excessive stomach distension, increase in salt loading, and presence of toxins. Hypothalamic-hindbrain OT pathways facilitate these aspects of OT-induced hypophagia. However, recent discoveries have implicated OT in modifications of feeding via reward circuits: OT has been found to differentially affect consumption of individual macronutrients in choice and no-choice paradigms. In this mini-review, we focus on presenting and interpreting evidence that defines OT as a key component of mechanisms that reduce eating for pleasure and shape macronutrient preferences. We also provide remarks on challenges in integrating the knowledge on physiological and pathophysiological states in which both OT activity and macronutrient preferences are affected.

  3. Comparison Between GOES-12 Overshooting-Top Detections, WSR-88D Radar Reflectivity, and Severe Storm Reports

    NASA Technical Reports Server (NTRS)

    Dworak, Richard; Bedka, Kristopher; Brunner, Jason; Feltz, Wayne

    2012-01-01

    Studies have found that convective storms with overshooting-top (OT) signatures in weather satellite imagery are often associated with hazardous weather, such as heavy rainfall, tornadoes, damaging winds, and large hail. An objective satellite-based OT detection product has been developed using 11-micrometer infrared window (IRW) channel brightness temperatures (BTs) for the upcoming R series of the Geostationary Operational Environmental Satellite (GOES-R) Advanced Baseline Imager. In this study, this method is applied to GOES-12 IRW data and the OT detections are compared with radar data, severe storm reports, and severe weather warnings over the eastern United States. The goals of this study are to 1) improve forecaster understanding of satellite OT signatures relative to commonly available radar products, 2) assess OT detection product accuracy, and 3) evaluate the utility of an OT detection product for diagnosing hazardous convective storms. The coevolution of radar-derived products and satellite OT signatures indicates that an OT often corresponds with the highest radar echo top and reflectivity maximum aloft. Validation of OT detections relative to composite reflectivity indicates an algorithm false-alarm ratio of 16%, with OTs within the coldest IRW BT range (less than 200 K) being the most accurate. A significant IRW BT minimum typically present with an OT is more often associated with heavy precipitation than a region with a spatially uniform BT. Severe weather was often associated with OT detections during the warm season (April September) and over the southern United States. The severe weather to OT relationship increased by 15% when GOES operated in rapid-scan mode, showing the importance of high temporal resolution for observing and detecting rapidly evolving cloud-top features. Comparison of the earliest OT detection associated with a severe weather report showed that 75% of the cases occur before severe weather and that 42% of collocated severe

  4. Effects of oxytocin on aggressive responding in healthy adult men.

    PubMed

    Alcorn, Joseph L; Green, Charles E; Schmitz, Joy; Lane, Scott D

    2015-12-01

    This study investigated the acute effects of oxytocin (OT) on human aggression using a well-established laboratory measure of state (reactive) aggression to test the hypothesis that OT would decrease the frequency of aggressive responding. In a within-subject design, 17 healthy male volunteers received placebo or 24 IU of intranasal OT. Aggression was measured using the Point Subtraction Aggression Paradigm at 30 min before and 30, 60, and 90 min after dose. Acute OT did not produce a significant main effect on aggressive behavior. OT attenuated the expected rise in diastolic blood pressure from morning to early afternoon observed under placebo, providing a possible indication of biological activity. Examination of individual differences showed that aggressive responding following OT dosing (but not placebo) was positively correlated with psychometric measures of interpersonal manipulation and anger (Pearson's r=0.57), indicating that higher scores on these antisocial personality traits were related to increased aggressive behavior following OT administration. These preliminary results stand in contrast to previous work on the prosocial effects of OT and highlight the need for further understanding of individual differences in aggression following OT administration. Such individual differences may have implications for the therapeutic use of OT in individuals with psychiatric disorders and dysfunctional social behavior. PMID:26241153

  5. A NOVEL GAMMAHERPESVIRUS IN NORTHERN FUR SEALS (CALLORHINUS URSINUS) IS CLOSELY RELATED TO THE CALIFORNIA SEA LION (ZALOPHUS CALIFORNIANUS) CARCINOMA-ASSOCIATED OTARINE HERPESVIRUS-1.

    PubMed

    Cortés-Hinojosa, Galaxia; Gulland, Frances M D; DeLong, Robert; Gelatt, Tom; Archer, Linda; Wellehan, James F X

    2016-01-01

    Otarine herpesvirus 1 (OtHV1) is strongly associated with California sea lion (CSL, Zalophus californianus) urogenital carcinoma, the most common cancer documented in marine mammals. In addition to CSL, OtHV1 has also been found in association with carcinoma in South American fur seals (Arctocephalus australis), demonstrating it can infect related species. Northern fur seals (NFS, Callorhinus ursinus) are sympatric with CSL, and copulation between these species has been observed; yet, there are no reports of urogenital carcinoma in NFS. We describe a new Otarine herpesvirus found in vaginal swabs from NFS, herein called OtHV4. Partial sequencing of the polymerase gene and the glycoprotein B gene revealed OtHV4 is closely related to OtHV1, with 95% homology in the region of polymerase sequenced, and phylogenetic analyses demonstrate that they are sister taxa. An OtHV4-specific hydrolysis probe quantitative PCR was developed and validated, and its use on vaginal swabs revealed 16 of 50 (32%) wild adult female NFS were positive for OtHV4. The identification of a virus highly similar to the carcinoma-associated OtHV1 in a sympatric species without carcinoma suggests that comparative genomics of OtHV1 and OtHV4 may identify candidate viral oncogenes. PMID:26555110

  6. Oxytocin Increases Invasive Properties of Endometrial Cancer Cells Through Phosphatidylinositol 3-Kinase/AKT-Dependent Up-Regulation of Cyclooxygenase-1, -2, and X-Linked Inhibitor of Apoptosis Protein1

    PubMed Central

    Déry, Marie-Claude; Chaudhry, Parvesh; Leblanc, Valérie; Parent, Sophie; Fortier, Anne-Marie; Asselin, Eric

    2011-01-01

    Traditionally, oxytocin (OT) is well known to play a crucial role in the regulation of cyclic changes in the uterus, implantation of the embryo, and parturition. Recently, an additional role for OT has been identified in several types of cancer cells in which OT acts as a growth regulator. In endometrial cancer cells, OT is known to efficiently inhibit cellular proliferation. In the present study, we show that OT increases invasiveness of human endometrial carcinoma (HEC) cells, which are otherwise resistant to the growth-inhibiting effects of OT. Using pharmacological inhibitors, invasion assay, RNA interference, and immunofluorescence, we found that OT enhances the invasive properties of HEC cells through up-regulation of X-linked inhibitor of apoptosis protein (XIAP), matrix-metalloproteinase 2 (MMP2), and matrix-metalloproteinase 14 (MMP14). In addition, we show that OT-mediated invasion is both cyclooxygenase 1 (PTGS1) and cyclooxygenase-2 (PTGS2) dependent via the phosphatidylinositol 3-kinase/AKT (PIK3/AKT) pathway. PTGS2 knockdown by shRNA resulted in XIAP down-regulation. We also show that OT receptor is overexpressed in grade I to III endometrial cancer. Taken together, our results describe for the first time a novel role for OT in endometrial cancer cell invasion. PMID:21816851

  7. Indication of a species in an extinction vortex: The ocellated turkey on the Yucatan peninsula, Mexico

    NASA Astrophysics Data System (ADS)

    Kampichler, Christian; Calmé, Sophie; Weissenberger, Holger; Arriaga-Weiss, Stefan Louis

    2010-11-01

    The ocellated turkey Meleagris ocellata (OT) is a large, unmistakable endemic bird of the Yucatan peninsula. The species has suffered a considerable loss of distributional area as well as local abundance between 1980 and 2000 and is classified as endangered according to Mexican norms. We applied Classification Trees and Random Forests in order to determine the factors that most closely explain the observed patterns of distribution and abundance loss, and to develop hypotheses that may guide measures for the protection of the OT. Among the most important predictors of change were variables corresponding to aspects of forest cover and variables on human population and small settlements. OT abundance in 1980, however, was by far the most important predictor for OT abundance change. This is an indication that the OT dynamics are governed by internal rather than by external factors. Medium and low abundances in 1980 inevitably led to a further decrease during the following years, which gives rise to the conclusion that the OT might find itself in an extinction vortex. We suggest the following hypothetical scenario for OT decline: migrant people from other Mexican states colonise forested regions in Yucatan; they establish small settlements; bushmeat hunting is important for their survival; the naïve OT is easy prey; hunting—together with beginning deforestation—reaches a certain level, and local OT abundance falls below a critical threshold; OT continues declining regardless of current social and environmental changes except where there is total protection of both the species and its habitat.

  8. Oxytocin and Estrogen Receptor β in the Brain: An Overview

    PubMed Central

    Acevedo-Rodriguez, Alexandra; Mani, Shaila K.; Handa, Robert J.

    2015-01-01

    Oxytocin (OT) is a neuropeptide synthesized primarily by neurons of the paraventricular and supraoptic nuclei of the hypothalamus. These neurons have axons that project into the posterior pituitary and release OT into the bloodstream to promote labor and lactation; however, OT neurons also project to other brain areas where it plays a role in numerous brain functions. OT binds to the widely expressed OT receptor (OTR), and, in doing so, it regulates homeostatic processes, social recognition, and fear conditioning. In addition to these functions, OT decreases neuroendocrine stress signaling and anxiety-related and depression-like behaviors. Steroid hormones differentially modulate stress responses and alter OTR expression. In particular, estrogen receptor β activation has been found to both reduce anxiety-related behaviors and increase OT peptide transcription, suggesting a role for OT in this estrogen receptor β-mediated anxiolytic effect. Further research is needed to identify modulators of OT signaling and the pathways utilized and to elucidate molecular mechanisms controlling OT expression to allow better therapeutic manipulations of this system in patient populations. PMID:26528239

  9. A NOVEL GAMMAHERPESVIRUS IN NORTHERN FUR SEALS (CALLORHINUS URSINUS) IS CLOSELY RELATED TO THE CALIFORNIA SEA LION (ZALOPHUS CALIFORNIANUS) CARCINOMA-ASSOCIATED OTARINE HERPESVIRUS-1.

    PubMed

    Cortés-Hinojosa, Galaxia; Gulland, Frances M D; DeLong, Robert; Gelatt, Tom; Archer, Linda; Wellehan, James F X

    2016-01-01

    Otarine herpesvirus 1 (OtHV1) is strongly associated with California sea lion (CSL, Zalophus californianus) urogenital carcinoma, the most common cancer documented in marine mammals. In addition to CSL, OtHV1 has also been found in association with carcinoma in South American fur seals (Arctocephalus australis), demonstrating it can infect related species. Northern fur seals (NFS, Callorhinus ursinus) are sympatric with CSL, and copulation between these species has been observed; yet, there are no reports of urogenital carcinoma in NFS. We describe a new Otarine herpesvirus found in vaginal swabs from NFS, herein called OtHV4. Partial sequencing of the polymerase gene and the glycoprotein B gene revealed OtHV4 is closely related to OtHV1, with 95% homology in the region of polymerase sequenced, and phylogenetic analyses demonstrate that they are sister taxa. An OtHV4-specific hydrolysis probe quantitative PCR was developed and validated, and its use on vaginal swabs revealed 16 of 50 (32%) wild adult female NFS were positive for OtHV4. The identification of a virus highly similar to the carcinoma-associated OtHV1 in a sympatric species without carcinoma suggests that comparative genomics of OtHV1 and OtHV4 may identify candidate viral oncogenes.

  10. The effect of acute stress exposure on ischemia and reperfusion injury in rat heart: role of oxytocin.

    PubMed

    Moghimian, Maryam; Faghihi, Mahdieh; Karimian, Seyed Morteza; Imani, Alireza

    2012-07-01

    Previous studies showed the protective effects of oxytocin (OT) on myocardial injury in ischemic and reperfused rat heart. Moreover, exposure to various stressors not only evokes sudden cardiovascular effects but also triggers the release of OT in the rat. The present study was aimed to evaluate the possible cardioprotective effects of endogenous OT released in response to stress (St), and effects of administration of exogenous OT on the ischemic-reperfused isolated heart of rats previously exposed to St. Wistar rats were divided into six groups: ischemia/reperfusion (IR); St: rats exposed to swim St for 10 min before anesthesia; St+atosiban (ATO): an OT receptor antagonist, was administered (1.5 mg/kg i.p.) prior to St; St+OT: OT was administered (0.03 mg/kg i.p.) prior to St; OT: OT was administrated prior to anesthesia; ATO was given prior to anesthesia. Isolated hearts were perfused with Krebs buffer solution by the Langendorff method and subjected to 30 min of regional ischemia followed by 60 min of reperfusion. The infarct size (IS) and creatine kinase MB isoenzyme (CK-MB) and lactate dehydrogenase (LDH) in coronary effluent were measured. Hemodynamic parameters were recorded throughout the experiment. The plasma concentrations of OT and corticosterone were significantly increased by St. Unexpectedly St decreased IR injury compared with the IR alone group. OT administration significantly inhibited myocardial injury, and administration of ATO with St abolished recovery of the rate pressure product, and increased IS and levels of CK-MB and LDH. These findings indicate that activation of cardiac OT receptors by OT released in response to St may participate in cardioprotection and inhibition of myocardial IR injury.

  11. Explaining left lateralization for words in the ventral occipitotemporal cortex.

    PubMed

    Seghier, Mohamed L; Price, Cathy J

    2011-10-12

    Reading is a uniquely human task and therefore any sign of neuronal activation that is specific to reading is of considerable interest. One intriguing observation is that ventral occipitotemporal (vOT) activation is more strongly left lateralized for written words than other visual stimuli. This has contributed to claims that left vOT plays a special role in reading. Here, we investigated whether left lateralized vOT responses for words were the consequence of visual feature processing, visual word form selectivity, or higher level language processing. Using fMRI in 82 skilled readers, our paradigm compared activation and lateralization for words and nonlinguistic stimuli during different tasks. We found that increased left lateralization for words relative to pictures was the consequence of reduced activation in right vOT rather than increased activation in left vOT. We also found that the determinants of lateralization varied with the subregion of vOT tested. In posterior vOT, lateralization depended on the spatial frequency of the visual inputs. In anterior vOT, lateralization depended on the semantic demands of the task. In middle vOT, lateralization depended on a combination of visual expertise in the right hemisphere and semantics in the left hemisphere. These results have implications for interpreting left lateralized vOT activation during reading. Specifically, left lateralized activation in vOT does not necessarily indicate an increase in left vOT processing but is instead a consequence of decreased right vOT function. Moreover, the determinants of lateralization include both visual and semantic factors depending on the subregion tested. PMID:21994390

  12. Explaining left lateralization for words in the ventral occipito-temporal cortex

    PubMed Central

    Seghier, Mohamed L; Price, Cathy J

    2011-01-01

    Summary Reading is a uniquely human task and therefore any sign that neuronal activation is specific to reading has been of considerable interest. One intriguing observation is that ventral occipito-temporal (vOT) activation is more strongly left lateralized for written words than other visual stimuli. This has contributed to claims that left vOT plays a special role in reading. Here, we investigated whether left lateralized vOT responses for words were the consequence of visual feature processing, visual word form selectivity or higher level language processing. Using fMRI in 82 skilled readers, our paradigm compared activation and lateralization for words and non-linguistic stimuli during different tasks. We found that increased left lateralization for words relative to pictures was the consequence of reduced activation in right vOT rather than increased activation in left vOT. We also found that the determinants of lateralization varied with the subregion of vOT tested. In posterior vOT, lateralization depended on the spatial frequency of the visual inputs. In anterior vOT, lateralization depended on the semantic demands of the task. In middle vOT, lateralization depended on a combination of visual expertise in the right hemisphere and semantics in the left hemisphere. These results have implications for interpreting left lateralized vOT activation during reading. Specifically, left lateralized activation in vOT does not necessarily indicate an increase in left vOT processing but is instead a consequence of decreased right vOT function. Moreover, the determinants of lateralization include both visual and semantic factors depending on the subregion tested. PMID:21994390

  13. [Therapeutic effects of oxytocin in autism: Current status of the research].

    PubMed

    Gauthier, C; Doyen, C; Amado, I; Lôo, H; Gaillard, R

    2016-02-01

    The neuropeptide oxytocin (OT) is an evolutionary highly conserved molecule that plays a part in the regulation of complex social cognition and behaviours. From a pathophysiological point of view, several studies have evidenced dysfunctions of the oxytocinergic system in autism spectrum disorders (ASD): a lowering of plasma OT and genetic or epigenetic anomalies of the OT receptor. Therefore, some authors have hypothesized that an abnormality in the OT neurotransmission may account for several features of autism and that a treatment restoring a normal OT pathway functioning could improve social abilities. OT administration has thus been used in clinical trials, especially in groups of subjects suffering from autism. Some studies found that OT decreased repetitive behaviours, enhanced emotional understanding of speech intonation, improved performance of the Reading the Mind in the Eyes Test and reinforced cooperation. Nevertheless, the findings of the OT administration studies on clinical samples show great diversity. The context, the personality and childhood experiences of the subject could be moderators influencing the effect of exogenous OT. Besides, three mechanisms could play a part in the action of OT on ASD social symptoms: anxiety reduction (with a lowering in the hypothalamic-pituitary-adrenal axis responsiveness and in the amygdale reactivity to social stimuli), increased affiliative motivation (involving the dopaminergic pathway and several regions of the social brain) and enhanced perceptual selectivity and social stimuli salience. To conclude, OT could be a promising molecule used as a treatment to promote social behaviours, helping individuals with ASD to develop new relationships. OT could be administered during a cognitive-behavioural therapy to reinforce the efficacy of such procedures. More studies are needed, on larger samples, to investigate the safety and efficacy of OT administration and to specify optimal dosages and characteristics of

  14. Conditioned same-sex partner preference in male rats is facilitated by oxytocin and dopamine: effect on sexually dimorphic brain nuclei.

    PubMed

    Triana-Del Rio, Rodrigo; Tecamachaltzi-Silvarán, Miriam B; Díaz-Estrada, Victor X; Herrera-Covarrubias, Deissy; Corona-Morales, Aleph A; Pfaus, James G; Coria-Avila, Genaro A

    2015-04-15

    Conditioned same-sex partner preference can develop in male rats that undergo cohabitation under the effects of quinpirole (QNP, D2 agonist). Herein, we assessed the development of conditioned same-sex social/sexual preference in males that received either nothing, saline, QNP, oxytocin (OT), or QNP+OT during cohabitation with another male (+) or single-caged (-). This resulted in the following groups: (1) Intact-, (2) Saline+, (3) QNP-, (4) OT-, (5) QNP+, (6) OT+ and (7) QNP/OT+. Cohabitation occurred during 24h in a clean cage with a male partner that bore almond scent on the back as conditioned stimulus. This was repeated every 4 days for a total of three trials. Social and sexual preference were assessed four days after the last conditioning trial in a drug-free test in which experimental males chose between the scented familiar male and a novel sexually receptive female. Results showed that males from groups Intact-, Saline+, QNP- and OT- displayed a clear preference for the female (opposite-sex), whereas groups QNP+, OT+ and QNP/OT+ displayed socio/sexual preference for the male partner (same-sex). In Experiment 2, the brains were processed for Nissl dye and the area size of two sexually dimorphic nuclei (SDN-POA and SON) was compared between groups. Males from groups OT-, OT+ and QNP/OT+ expressed a smaller SDN-POA and groups QNP+ and QNP/OT+ expressed a larger SON. Accordingly, conditioned same-sex social/sexual partner preference can develop during cohabitation under enhanced D2 or OT activity but such preference does not depend on the area size of those sexually dimorphic nuclei.

  15. Medial nucleus tractus solitarius oxytocin receptor signaling and food intake control: the role of gastrointestinal satiation signal processing

    PubMed Central

    Alhadeff, Amber L.; Grill, Harvey J.

    2015-01-01

    Central oxytocin (OT) administration reduces food intake and its effects are mediated, in part, by hindbrain oxytocin receptor (OT-R) signaling. The neural substrate and mechanisms mediating the intake inhibitory effects of hindbrain OT-R signaling are undefined. We examined the hypothesis that hindbrain OT-R-mediated feeding inhibition results from an interaction between medial nucleus tractus solitarius (mNTS) OT-R signaling and the processing of gastrointestinal (GI) satiation signals by neurons of the mNTS. Here, we demonstrated that mNTS or fourth ventricle (4V) microinjections of OT in rats reduced chow intake in a dose-dependent manner. To examine whether the intake suppressive effects of mNTS OT-R signaling is mediated by GI signal processing, rats were injected with OT to the 4V (1 μg) or mNTS (0.3 μg), followed by self-ingestion of a nutrient preload, where either treatment was designed to be without effect on chow intake. Results showed that the combination of mNTS OT-R signaling and GI signaling processing by preload ingestion reduced chow intake significantly and to a greater extent than either stimulus alone. Using enzyme immunoassay, endogenous OT content in mNTS-enriched dorsal vagal complex (DVC) in response to ingestion of nutrient preload was measured. Results revealed that preload ingestion significantly elevated endogenous DVC OT content. Taken together, these findings provide evidence that mNTS neurons are a site of action for hindbrain OT-R signaling in food intake control and that the intake inhibitory effects of hindbrain mNTS OT-R signaling are mediated by interactions with GI satiation signal processing by mNTS neurons. PMID:25740340

  16. Medial nucleus tractus solitarius oxytocin receptor signaling and food intake control: the role of gastrointestinal satiation signal processing.

    PubMed

    Ong, Zhi Yi; Alhadeff, Amber L; Grill, Harvey J

    2015-05-01

    Central oxytocin (OT) administration reduces food intake and its effects are mediated, in part, by hindbrain oxytocin receptor (OT-R) signaling. The neural substrate and mechanisms mediating the intake inhibitory effects of hindbrain OT-R signaling are undefined. We examined the hypothesis that hindbrain OT-R-mediated feeding inhibition results from an interaction between medial nucleus tractus solitarius (mNTS) OT-R signaling and the processing of gastrointestinal (GI) satiation signals by neurons of the mNTS. Here, we demonstrated that mNTS or fourth ventricle (4V) microinjections of OT in rats reduced chow intake in a dose-dependent manner. To examine whether the intake suppressive effects of mNTS OT-R signaling is mediated by GI signal processing, rats were injected with OT to the 4V (1 μg) or mNTS (0.3 μg), followed by self-ingestion of a nutrient preload, where either treatment was designed to be without effect on chow intake. Results showed that the combination of mNTS OT-R signaling and GI signaling processing by preload ingestion reduced chow intake significantly and to a greater extent than either stimulus alone. Using enzyme immunoassay, endogenous OT content in mNTS-enriched dorsal vagal complex (DVC) in response to ingestion of nutrient preload was measured. Results revealed that preload ingestion significantly elevated endogenous DVC OT content. Taken together, these findings provide evidence that mNTS neurons are a site of action for hindbrain OT-R signaling in food intake control and that the intake inhibitory effects of hindbrain mNTS OT-R signaling are mediated by interactions with GI satiation signal processing by mNTS neurons.

  17. Results of tests in the NASA/LaRC 31-inch CFHT on an 0.010-scale model (32-OT) of the space shuttle configuration 3 to determine the RCS jet flowfield interaction effects on aerodynamic characteristics (IA60/0A105), volume 2

    NASA Technical Reports Server (NTRS)

    Thornton, D. E.

    1974-01-01

    Tests were conducted in the NASA Langley Research Center 31-inch continuous flow hypersonic wind tunnel from 14 February to 22 February 1974, to determine RCS jet interaction effect on the hypersonic aerodynamic and stability and control characteristics prior to RTLS abort separation. The model used was an 0.010-scale replica of the space shuttle vehicle configuration 3. Hypersonic stability data were obtained from tests at Mach 10.3 and dynamic pressure of 150 psf for the intergrated orbiter and external tank and the orbiter alone. RCS modes of pitch, yaw, and roll at free flight dynamic pressure simulation of 7, 20, and 50 psf were investigated. The effects of speedbrake, bodyflap, elevon, and aileron deflections were also investigated.

  18. Results of tests in the NASA/LaRC 31-inch CFHT on an 0.010-scale model (32-OT) of the space shuttle configuration 3 to determine the RCS jet flowfield interaction effects on aerodynamic characteristics (IA60/0A105), volume 1

    NASA Technical Reports Server (NTRS)

    Thornton, D. E.

    1974-01-01

    Tests were conducted in the 31-inch continuous Flow Hypersonic Wind Tunnel to determine RCS jet interaction effect on the hypersonic aerodynamic and stability and control characteristics prior to RTLS abort separation. The model used was an 0.010-scale replica of the Space Shuttle Vehicle Configuration 3. Hypersonic stability data were obtained from tests at Mach 10.3 and dynamic pressure of 150 psf for the integrated Orbiter and external tank and the Orbiter alone. RCS modes of pitch, yaw, and roll at free flight dynamic pressure simulation of 7, 20, and 50 psf were investigated. The effects of speedbrake, bodyflap, elevon, and airleron deflections were also investigated.

  19. Airloads investigation of an 0.030-scale model of the space shuttle vehicle 140A/B launch configuration (model 47-OTS) in the arc 11-foot unitary plan wind tunnel for Mach range 0.6 to 1.4 (IA14A), Volume 2

    NASA Technical Reports Server (NTRS)

    Gillins, R. L.

    1975-01-01

    Results of tests conducted on an 0.030-scale launch configuration model of the space shuttle vehicle 140A/B in the NASA/ARC 11-foot unitary plan wind tunnel are presented. Aerodynamic loads data were obtained at Mach numbers from 0.6 to 1.4. Surface pressure distributions were obtained simultaneously with six-component stability and control force data on the complete launch configuration. The configuration consisted of the orbiter, an external tank, two solid rocket boosters, and associated intercomponent attach hardware. Angles of attack and sideslip from -10 degrees to +10 degrees were investigated.

  20. Results of tests in the NASA/LARC 31-inch CFHT on an 0.010-scale model (32-OT) of the space shuttle configuration 3 to determine the RCS jet flowfield interaction effects on aerodynamic characteristics (IA60/OA105), volume 1

    NASA Technical Reports Server (NTRS)

    Thornton, D. E.

    1974-01-01

    Tests were conducted in the NASA Langley Research Center 31-inch continuous Flow Hypersonic Wind Tunnel to determine RCS jet interaction effect on the hypersonic aerodynamic and stability and control characteristics prior to return to launch site (RTLS) abort separation. The model used was an 0.010-scale replica of the Space Shuttle Vehicle Configuration 3. Hypersonic stability data were obtained from tests at Mach 10.3 and dynamic pressure of 150 psf for the integrated Orbiter and external tank and the Orbiter alone. RCS modes of pitch, yaw, and roll at free flight dynamic pressure simulation of 7, 20, and 50 psf were investigated. The effects of speedbrake, bodyflap, elevon, and aileron deflections were also investigated.

  1. Results of a jet plume effects test on Rockwell International integrated space shuttle vehicle using a vehicle 5 configuration 0.02-scale model (88-OTS) in the 11 by 11 foot leg of the NASA/Ames Research Center unitary plan wind tunnel (IA19), volume 1

    NASA Technical Reports Server (NTRS)

    Nichols, M. E.

    1975-01-01

    Results are presented of jet plume effects test IA19 using a vehicle 5 configuration integrated space shuttle vehicle 0.02-scale model in the NASA/Ames Research Center 11 x 11-foot leg of the unitary plan wind tunnel. The jet plume power effects on the integrated vehicle static pressure distribution were determined along with elevon, main propulsion system nozzle, and solid rocket booster nozzle effectiveness and elevon hinge moments.

  2. Results of an investigation of jet plume effects on an 0.010-scale model (75-OTS) of the space shuttle integrated vehicle in the 9 x 7-foot leg of the NASA/Ames unitary wind tunnel (IA82B), volume 1. [an exhaust flow simulation

    NASA Technical Reports Server (NTRS)

    Hawthorne, P. J.

    1976-01-01

    The base pressure environment was investigated for the first and second stage mated vehicle in a supersonic flow field from Mach 1.55 through 2.20 with simulated rocket engine exhaust plumes. The pressure environment was investigated for the orbiter at various vent port locations at these same freestream conditions. The Mach number environment around the base of the model with rocket plumes simulated was examined. Data were obtained at angles of attack from -4 deg through +4 deg at zero yaw, and at yaw angles from -4 deg through +4 deg at zero angle of attack, with rocket plume sizes varying from smaller than nominal to much greater than nominal. Failed orbiter engine data were also obtained. Elevon hinge moments and wing panel load data were obtained during all runs. Photographs of the tested configurations are shown.

  3. Results of tests OA26 and IA16 in the NASA/ARC 3.5-foot hypersonic wind tunnel on an 0.015-scale model (36-OTS) of the space shuttle configuration 140A/B to obtain pressures for venting analysis

    NASA Technical Reports Server (NTRS)

    Spangler, R. H.; Thornton, D. E.; Polek, T. E.

    1974-01-01

    Tests were conducted, from November 15 to December 4, 1973, to obtain surface pressure data on an 0.015-scale replica of the Space Shuttle Vehicle 4. Data were obtained at Mach numbers of 5.3, 7.4, and 10.3, to support the venting analysis for both launch and entry conditions. These tests were the final tests in a series covering a Mach number range from 0.6 to 10.3. The model was instrumented with pressure orifices in the vicinity of the cargo bay door hinge and parting lines, and on the side of the fuselage at the crew compartment, and below the orbital maneuvering system pods at the aft compartment. The model was tested at angles of attack and sideslip consistent with expected divergencies from the nominal trajectory.

  4. Results of a pressure loads investigation on a 0.030-scale model (47-OTS) of the integrated space shuttle vehicle configuration 5 in the NASA Ames Research Center 9 by 7 foot leg of the unitary plan wind tunnel (IA81B), volume 1

    NASA Technical Reports Server (NTRS)

    Chee, E.

    1975-01-01

    The investigations of pressure distributions are presented for aeroloads analysis at Mach numbers from 1.55 through 2.5. Angles of attack and sideslip varied from -6 to +6 degrees. Photographs of wind tunnel models are shown.

  5. Convective overshooting top detection with MSG SEVIRI, Himawari-8 AHI, and CloudSat CPR data

    NASA Astrophysics Data System (ADS)

    Im, J.; Kim, M.; Park, S.

    2015-12-01

    Overshooting Tops (OTs) are the clouds that penetrate into the tropopause and grow to the bottom of stratosphere at the top layer of cumulonimbus with very strong updraft. Severe weather conditions such as ground lightning, large hail, strong winds, and heavy rainfall can cause in the cumulonimbus clouds with OTs, with turbulence and lightning occurring very frequently in the area near OTs. In terms of aviation operations, OTs are a very important risk factor. According to Federal Aviation Administration, 509 cases of 4,326 weather-related events from 1992 to 2001were caused by turbulences. The detection of OTs is important to predict the degree and location of severe weather conditions such as turbulence, lightning, and thunderstorms. There are two methods widely used to detect OTs with multispectral images. One is the Water Vapor-InfraRed window channel Brightness Temperature Difference (WV-IRW BTD), which uses the differences in brightness temperatures at an infrared channel (about 11 ㎛). The other approach is the InfraRed Window texture (IRW-texture) method, which is based on the characteristics of OTs that appear a pixel group with low temperatures. The typical IRW-texture algorithm uses simple thresholds to detect OTs, whereas this research proposes an advanced approach based on machine learning techniques such as decision trees, random forest (RF), and support vector machines (SVM) with various variables from geostationary satellite data such as MSG SEVIRI (over Africa) and Himawari AHI (over East Asia) so as to improve the detection of OTs. OT and non-OT samples (e.g. other types of clouds such as stratus and cirrus) were extracted using the CloudSat cloud profiling radar (CPR) and SEVIRI (and Himawari) imagery. Results show that RF produced the best performance in detection of OTs yielding an overall accuracy of 98.33% and a false alarm rate of 9.01%. The user's accuracies of OT and non-OT were similar, whereas the producer's accuracy of non-OT was

  6. The Interactive Account of ventral occipitotemporal contributions to reading

    PubMed Central

    Price, Cathy J.; Devlin, Joseph T.

    2011-01-01

    The ventral occipitotemporal cortex (vOT) is involved in the perception of visually presented objects and written words. The Interactive Account of vOT function is based on the premise that perception involves the synthesis of bottom-up sensory input with top-down predictions that are generated automatically from prior experience. We propose that vOT integrates visuospatial features abstracted from sensory inputs with higher level associations such as speech sounds, actions and meanings. In this context, specialization for orthography emerges from regional interactions without assuming that vOT is selectively tuned to orthographic features. We discuss how the Interactive Account explains left vOT responses during normal reading and developmental dyslexia; and how it accounts for the behavioural consequences of left vOT damage. PMID:21549634

  7. [Levels of beta-endorphin in response to exercise and overtraining].

    PubMed

    Cunha, Giovani S; Ribeiro, Jerri L; Oliveira, Alvaro R

    2008-06-01

    Overtraining (OT) is a complex and multifactorial sport phenomenon, and there is no independent marker that can diagnose OT. Interestingly, some symptoms of OT are related to beta-endorphin (beta-end(1-31)) effects. Some of its effects, such as analgesia, increasing lactate tolerance, and exercise-induced euphoria, are important for training. These effects can be reverted by detraining or OT, which may cause decrease in performance, reduced load tolerance, and depression. The main stimulus for beta-end(1-31) secretion is to exercise because its secretion is volume/intensity dependent for both aerobic and anaerobic exercise. Excess training, however, may reduce beta-end(1-31) concentrations, thus altering its beneficial effects. Therefore, beta-end(1-31) could be used as an additional OT marker, mainly because its effects are strongly related to OT symptoms. PMID:18604371

  8. Low-dose oxytocin delivered intranasally with Breath Powered device affects social-cognitive behavior: a randomized four-way crossover trial with nasal cavity dimension assessment

    PubMed Central

    Quintana, D S; Westlye, L T; Rustan, Ø G; Tesli, N; Poppy, C L; Smevik, H; Tesli, M; Røine, M; Mahmoud, R A; Smerud, K T; Djupesland, P G; Andreassen, O A

    2015-01-01

    Despite the promise of intranasal oxytocin (OT) for modulating social behavior, recent work has provided mixed results. This may relate to suboptimal drug deposition achieved with conventional nasal sprays, inter-individual differences in nasal physiology and a poor understanding of how intranasal OT is delivered to the brain in humans. Delivering OT using a novel ‘Breath Powered' nasal device previously shown to enhance deposition in intranasal sites targeted for nose-to-brain transport, we evaluated dose-dependent effects on social cognition, compared response with intravenous (IV) administration of OT, and assessed nasal cavity dimensions using acoustic rhinometry. We adopted a randomized, double-blind, double-dummy, crossover design, with 16 healthy male adults completing four single-dose treatments (intranasal 8 IU (international units) or 24 IU OT, 1 IU OT IV and placebo). The primary outcome was social cognition measured by emotional ratings of facial images. Secondary outcomes included the pharmacokinetics of OT, vasopressin and cortisol in blood and the association between nasal cavity dimensions and emotional ratings. Despite the fact that all the treatments produced similar plasma OT increases compared with placebo, there was a main effect of treatment on anger ratings of emotionally ambiguous faces. Pairwise comparisons revealed decreased ratings after 8 IU OT in comparison to both placebo and 24 IU OT. In addition, there was an inverse relationship between nasal valve dimensions and anger ratings of ambiguous faces after 8-IU OT treatment. These findings provide support for a direct nose-to-brain effect, independent of blood absorption, of low-dose OT delivered from a Breath Powered device. PMID:26171983

  9. Parental oxytocin and early caregiving jointly shape children's oxytocin response and social reciprocity.

    PubMed

    Feldman, Ruth; Gordon, Ilanit; Influs, Moran; Gutbir, Tamar; Ebstein, Richard P

    2013-06-01

    Oxytocin (OT) has an important role in bond formation and social reciprocity, and animal studies indicate that OT functioning is transferred from parent to child through patterns of parental care. Perspectives on attachment suggest that the individual's various attachment bonds are underpinned by the oxytocinergic system. However, prospective human studies that demonstrate the cross-generation transfer of OT as mediated by early caregiving and its impact on children's multiple attachments are lacking. To address these concerns, the current study included 160 mothers and fathers and their firstborn child who participated in a 3-year longitudinal study. At the first and sixth postpartum months, parents' plasma OT was assayed, parent-infant interactions were videotaped and micro-coded, and allelic variations on the OXTR(rs2254298, rs1042778) and CD38rs3796863 genes were measured. At 3 years, parents' and child's salivary OT was assessed and children's social reciprocity observed during interactions with mother, father, and their first best friend. Parents' OT levels were individually stable across the 3-year period, correlated with low-risk OXTR and CD38 alleles, and predicted child OT. Child's social reciprocity with friend was associated with child OT levels, mother's OT-related genes and hormones, and mother-child reciprocity, but not with father's genes, hormones, or behavior. A cross-generation gene-by-environment effect emerged, with low child OT levels predicted by the interaction of maternal high-risk CD38 allele and diminished maternal care in infancy. These results demonstrate individual stability in peripheral OT across several years and describe a cross-generation transfer of OT through caregiving in humans within a prospective longitudinal design. Consistent with other mammals, biobehavioral experiences within the parent-infant bond shape children's affiliative biology and social behavior across multiple attachments. Our findings bear important

  10. Classification of odontogenic cysts and tumors – Antecedents

    PubMed Central

    Imran, Aesha; Jayanthi, P; Tanveer, Shahela; Gobu, Sreeja C

    2016-01-01

    Pierre Paul Broca produced a monograph on tumor classification which also included the classification of odontogenic tumors (OTs). The terminology used to describe malignant epithelial OTs has varied since the World Health Organization published the initial consensus on the taxonomy of OTs. Minor changes were introduced in the second edition. It is only in the very recent years that additional knowledge has accumulated and refined the classification. This review emphasizes on reasons for modification by each author and the recent acceptance.

  11. Yttrium Siloxide Complexes Bearing Terminal Methyl Ligands: Molecular Models for Ln-CH3 Terminated Silica Surfaces.

    PubMed

    Dettenrieder, Nicole; Dietrich, H Martin; Maichle-Mössmer, Cäcilia; Anwander, Reiner

    2016-09-01

    Surface organometallic chemistry (SOMC) on silica materials is a prominent approach for the generation of highly active heterogenized polymerization catalysts. Despite advanced methods of characterization, the elucidation of the catalytically active surface species remains a challenging task. Alkylated rare-earth metal siloxide complexes can be regarded as molecular models of respective covalently bonded alkylated surface species, primarily used for 1,3-diene polymerization. Here, we performed both salt metathesis reactions of [Y(MMe4 )3 ] (M = Al, Ga) with [K{OSi(OtBu)3 }] and alkylation reactions of [Y{OSi(OtBu)3 }3 ]2 with AlMe3 . The obtained complexes [Y(CH3 )[(AlMe2 ){OSi(OtBu)3 }2 ](AlMe4 )]2 , [Y(CH3 )[(AlMe2 ){OSi(OtBu)3 }2 ]-{OSi(OtBu)3 }], [Y{OSi(OtBu)3 }3 (μ-Me)Y(μ-Me)2 Y{OSi(OtBu)3 }2 (AlMe4 )], and [Y(CH3 )(GaMe4 ){OSi(OtBu)3 }]2 represent rare examples of organoyttrium species with terminal methyl groups. The formation and purity of the mixed methyl/siloxy yttrium complexes could be enhanced by treating [Y(MMe4 )3 ] with [K(MMe2 ){OSi(OtBu)3 }2 ]n (M=Al: n=2; M=Ga: n=∞). Complexes [K(MMe2 ){OSi(OtBu)3 }2 ]n were obtained by addition of [K{OSi(OtBu)3 }] to [Me2 M{OSi(OtBu)3 }]2 . Deeper insight into the fluxional behavior of the mixed methyl/siloxy yttrium complexes in solution was gained by (1) H and (13) C NMR spectroscopic studies at variable temperature and (1) H-(89) Y HSQC NMR spectroscopy. PMID:27492852

  12. Effect of environmental pollutants on oxytocin synthesis and secretion from corpus luteum and on contractions of uterus from pregnant cows

    SciTech Connect

    Mlynarczuk, Jaroslaw; Wrobel, Michal H.; Kotwica, Jan

    2010-09-15

    Chloro-organic compounds are persistent environmental pollutants and affect many reproductive processes. Oxytocin (OT) synthesized in luteal cells is a local regulator of ovarian activity and uterine contractions. Therefore the effect of xenobiotics on the OT prohormone synthesis, secretion of OT and progesterone (P4) from luteal cells and on myometrial contractions during early pregnancy in cows was investigated. Luteal cells and myometrial strips from a cow at early pregnancy were treated with polychlorinated biphenyl 77 (PCB 77), dichlorodiphenyltrichloroethane (DDT), dichlorodiphenyldichloroethylene (DDE) and hexachlorocyclohexane (HCH) (1 or 10 ng/ml). The mRNA expression of neurophysin-I/oxytocin (NP-I/OT) and peptidyl-glycine-{alpha}-amidating mono-oxygenase (PGA) and concentration of OT and P4 were determined by RT-PCR and EIA, respectively. Moreover, the effect of xenobiotics given with P4 (12 ng/ml) on the basal and OT (10{sup -7} M) stimulated contractions of myometrial strips was studied. Xenobiotics increased (P < 0.05) OT secretion but DDE only stimulated P4 secretion. The ratio of P4 to OT in culture medium was decreased by all xenobiotics during 9-12 weeks of pregnancy. All xenobiotics, except HCH, increased (P < 0.05) mRNA expression of NP-I/OT during all stages of pregnancy and all treatments decreased (P < 0.05) expression of mRNA for PGA during 9-12 weeks of pregnancy. Myometrial strips were relaxed (P < 0.01) after pre-incubation with P4, while each of the xenobiotics jointly with P4 increased (P < 0.01) myometrial contractions. In conclusion, the xenobiotics used increased both expression of mRNA for genes involved in OT synthesis and secretion of OT from luteal cells. This decreases the ratio of P4 to OT and presumably, in this manner, the chloro-organic compounds can influence uterine contractions and enhance risk of abortions in pregnant females.

  13. Classification of odontogenic cysts and tumors – Antecedents

    PubMed Central

    Imran, Aesha; Jayanthi, P; Tanveer, Shahela; Gobu, Sreeja C

    2016-01-01

    Pierre Paul Broca produced a monograph on tumor classification which also included the classification of odontogenic tumors (OTs). The terminology used to describe malignant epithelial OTs has varied since the World Health Organization published the initial consensus on the taxonomy of OTs. Minor changes were introduced in the second edition. It is only in the very recent years that additional knowledge has accumulated and refined the classification. This review emphasizes on reasons for modification by each author and the recent acceptance. PMID:27601821

  14. Classification of odontogenic cysts and tumors - Antecedents.

    PubMed

    Imran, Aesha; Jayanthi, P; Tanveer, Shahela; Gobu, Sreeja C

    2016-01-01

    Pierre Paul Broca produced a monograph on tumor classification which also included the classification of odontogenic tumors (OTs). The terminology used to describe malignant epithelial OTs has varied since the World Health Organization published the initial consensus on the taxonomy of OTs. Minor changes were introduced in the second edition. It is only in the very recent years that additional knowledge has accumulated and refined the classification. This review emphasizes on reasons for modification by each author and the recent acceptance.

  15. Manipulation of the oxytocin system alters social behavior and attraction in pair-bonding primates, Callithrix penicillata

    PubMed Central

    Smith, Adam S.; Ågmo, Anders; Birnie, Andrew K.; French, Jeffrey A.

    2010-01-01

    The establishment and maintenance of stable, long-term male-female relationships, or pair bonds, are marked by high levels of mutual attraction, selective preference for the partner, and high rates of sociosexual behavior. Central oxytocin (OT) affects social preference and partner-directed social behavior in rodents, but the role of this neuropeptide has yet to be studied in heterosexual primate relationships. The present study evaluated whether the OT system plays a role in the dynamics of social behavior and partner preference during the first three weeks of cohabitation in male and female marmosets, Callithrix penicillata. OT activity was stimulated by intranasal administration of OT, and inhibited by oral administration of a non-peptide OT-receptor antagonist (L-368,899; Merck). Social behavior throughout the pairing varied as a function of OT treatment. Compared to controls, marmosets initiated huddling with their social partner more often after OT treatments but reduced proximity and huddling after OT antagonist treatments. OT antagonist treatment also eliminated food sharing between partners. During the 24-h preference test, all marmosets interacted more with an opposite-sex stranger than with the partner. By the third-week preference test, marmosets interacted with the partner and stranger equally with the exception that intranasal-OT treatments facilitated initial partner-seeking behavior over initial contact with the stranger. Our findings demonstrate that pharmacological manipulations of OT activity alter partner-directed social behavior during pair interactions, suggesting that central OT may facilitate the process of pair-bond formation and social relationships in marmoset monkeys. PMID:20025881

  16. Oxytocin mechanisms of stress response and aggression in a territorial finch.

    PubMed

    Goodson, James L; Schrock, Sara E; Kingsbury, Marcy A

    2015-03-15

    All jawed vertebrates produce a form of oxytocin (OT), and in birds, mammals and fish, OT is strongly associated with affiliation. However, remarkably few data are available on the roles of OT and OT receptors (OTRs) in aggression. Because OT and OTRs exert anxiolytic effects in mammals (although context-specific) and modulate stress coping, we hypothesized that OTR activation is at least permissive for territorial aggression. Indeed, we find that peripheral injections of an OTR antagonist significantly reduce male-male and female-female aggression in a highly territorial finch. This finding suggests the hypothesis that aggression is accompanied by an increase in transcriptional (Fos) activity of OT neurons, but contrary to this hypothesis, we find that dominant male residents do not elevate OT-Fos colocalization following an aggressive encounter and that OT-Fos colocalization in the preoptic area and hypothalamus correlates negatively with aggression. Furthermore, OT-Fos colocalization increases dramatically in males that were aggressively subjugated or pursued by a human hand, likely reflecting OT modulation of stress response. Because OT inhibits the hypothalamo-pituitary-adrenal axis, the antagonist effects may reflect the fact that aggressive birds and mammals tend to be hyporesponsive to stress. If this is correct, then 1) the observed effects of OTR antagonism may reflect alterations in corticosterone feedback to the brain rather than centrally mediated OTR effects, and 2) the negative correlation between OT-Fos colocalization and aggression may reflect the fact that more aggressive, stress hyporesponsive males require less inhibition of the hypothalamo-pituitary-adrenal axis than do less aggressive males, despite the requirement of that inhibition for the normal display of aggression.

  17. Effects of water concentration in the coating solution on the wall relaxation rate of octadecyltrichlorosilane coated rubidium vapor cells

    SciTech Connect

    Zhang, Guiying; Wei, Lihua; Wang, Meiling; Zhao, Kaifeng

    2015-01-28

    High quality anti-relaxation surface coatings for atomic vapor cells are essential for the preservation of atomic spin coherence and the enhancement of measurement sensitivity. In this paper, we studied the effects of water concentration in octadecyltrichlorosilane (OTS) coating solution on the relaxation rate and its reproducibility of OTS coated Rubidium vapor cells. We found that appropriate water concentration can improve the anti-relaxation performance of OTS coated cells.

  18. Yttrium Siloxide Complexes Bearing Terminal Methyl Ligands: Molecular Models for Ln-CH3 Terminated Silica Surfaces.

    PubMed

    Dettenrieder, Nicole; Dietrich, H Martin; Maichle-Mössmer, Cäcilia; Anwander, Reiner

    2016-09-01

    Surface organometallic chemistry (SOMC) on silica materials is a prominent approach for the generation of highly active heterogenized polymerization catalysts. Despite advanced methods of characterization, the elucidation of the catalytically active surface species remains a challenging task. Alkylated rare-earth metal siloxide complexes can be regarded as molecular models of respective covalently bonded alkylated surface species, primarily used for 1,3-diene polymerization. Here, we performed both salt metathesis reactions of [Y(MMe4 )3 ] (M = Al, Ga) with [K{OSi(OtBu)3 }] and alkylation reactions of [Y{OSi(OtBu)3 }3 ]2 with AlMe3 . The obtained complexes [Y(CH3 )[(AlMe2 ){OSi(OtBu)3 }2 ](AlMe4 )]2 , [Y(CH3 )[(AlMe2 ){OSi(OtBu)3 }2 ]-{OSi(OtBu)3 }], [Y{OSi(OtBu)3 }3 (μ-Me)Y(μ-Me)2 Y{OSi(OtBu)3 }2 (AlMe4 )], and [Y(CH3 )(GaMe4 ){OSi(OtBu)3 }]2 represent rare examples of organoyttrium species with terminal methyl groups. The formation and purity of the mixed methyl/siloxy yttrium complexes could be enhanced by treating [Y(MMe4 )3 ] with [K(MMe2 ){OSi(OtBu)3 }2 ]n (M=Al: n=2; M=Ga: n=∞). Complexes [K(MMe2 ){OSi(OtBu)3 }2 ]n were obtained by addition of [K{OSi(OtBu)3 }] to [Me2 M{OSi(OtBu)3 }]2 . Deeper insight into the fluxional behavior of the mixed methyl/siloxy yttrium complexes in solution was gained by (1) H and (13) C NMR spectroscopic studies at variable temperature and (1) H-(89) Y HSQC NMR spectroscopy.

  19. Classification of odontogenic cysts and tumors - Antecedents.

    PubMed

    Imran, Aesha; Jayanthi, P; Tanveer, Shahela; Gobu, Sreeja C

    2016-01-01

    Pierre Paul Broca produced a monograph on tumor classification which also included the classification of odontogenic tumors (OTs). The terminology used to describe malignant epithelial OTs has varied since the World Health Organization published the initial consensus on the taxonomy of OTs. Minor changes were introduced in the second edition. It is only in the very recent years that additional knowledge has accumulated and refined the classification. This review emphasizes on reasons for modification by each author and the recent acceptance. PMID:27601821

  20. Low-dose oxytocin delivered intranasally with Breath Powered device affects social-cognitive behavior: a randomized four-way crossover trial with nasal cavity dimension assessment.

    PubMed

    Quintana, D S; Westlye, L T; Rustan, Ø G; Tesli, N; Poppy, C L; Smevik, H; Tesli, M; Røine, M; Mahmoud, R A; Smerud, K T; Djupesland, P G; Andreassen, O A

    2015-01-01

    Despite the promise of intranasal oxytocin (OT) for modulating social behavior, recent work has provided mixed results. This may relate to suboptimal drug deposition achieved with conventional nasal sprays, inter-individual differences in nasal physiology and a poor understanding of how intranasal OT is delivered to the brain in humans. Delivering OT using a novel 'Breath Powered' nasal device previously shown to enhance deposition in intranasal sites targeted for nose-to-brain transport, we evaluated dose-dependent effects on social cognition, compared response with intravenous (IV) administration of OT, and assessed nasal cavity dimensions using acoustic rhinometry. We adopted a randomized, double-blind, double-dummy, crossover design, with 16 healthy male adults completing four single-dose treatments (intranasal 8 IU (international units) or 24 IU OT, 1 IU OT IV and placebo). The primary outcome was social cognition measured by emotional ratings of facial images. Secondary outcomes included the pharmacokinetics of OT, vasopressin and cortisol in blood and the association between nasal cavity dimensions and emotional ratings. Despite the fact that all the treatments produced similar plasma OT increases compared with placebo, there was a main effect of treatment on anger ratings of emotionally ambiguous faces. Pairwise comparisons revealed decreased ratings after 8 IU OT in comparison to both placebo and 24 IU OT. In addition, there was an inverse relationship between nasal valve dimensions and anger ratings of ambiguous faces after 8-IU OT treatment. These findings provide support for a direct nose-to-brain effect, independent of blood absorption, of low-dose OT delivered from a Breath Powered device. PMID:26171983

  1. Sniff and mimic - Intranasal oxytocin increases facial mimicry in a sample of men.

    PubMed

    Korb, Sebastian; Malsert, Jennifer; Strathearn, Lane; Vuilleumier, Patrik; Niedenthal, Paula

    2016-08-01

    The neuropeptide oxytocin (OT) has many potential social benefits. For example, intranasal administration of OT appears to trigger caregiving behavior and to improve the recognition of emotional facial expressions. But the mechanism for these effects is not yet clear. Recent findings relating OT to action imitation and to the visual processing of the eye region of faces point to mimicry as a mechanism through which OT improves processing of emotional expression. To test the hypothesis that increased levels of OT in the brain enhance facial mimicry, 60 healthy male participants were administered, in a double-blind between-subjects design, 24 international units (IUs) of OT or placebo (PLA) through nasal spray. Facial mimicry and emotion judgments were recorded in response to movie clips depicting changing facial expressions. As expected, facial mimicry was increased in the OT group, but effects were strongest for angry infant faces. These findings provide further evidence for the importance of OT in social cognitive skills, and suggest that facial mimicry mediates the effects of OT on improved emotion recognition. PMID:27283377

  2. Time course of the estradiol-dependent induction of oxytocin receptor binding in the ventromedial hypothalamic nucleus of the rat

    SciTech Connect

    Johnson, A.E.; Ball, G.F.; Coirini, H.; Harbaugh, C.R.; McEwen, B.S.; Insel, T.R. )

    1989-09-01

    Oxytocin (OT) transmission is involved in the steroid-dependent display of sexual receptivity in rats. One of the biochemical processes stimulated by the ovarian steroid 17 beta-estradiol (E2) that is relevant to reproduction is the induction of OT receptor binding in the ventromedial hypothalamic nucleus (VMN). The purpose of these experiments was to determine if E2-induced changes in OT receptor binding in the VMN occur within a time frame relevant to cyclic changes in ovarian steroid secretion. OT receptor binding was measured in the VMN of ovariectomized rats implanted for 0-96 h with E2-containing Silastic capsules. The rate of decay of OT receptor binding was measured in another group of animals 6-48 h after capsule removal. Receptors were labeled with the specific OT receptor antagonist ({sup 125}I)d(CH2)5(Tyr(Me)2,Thr4,Tyr-NH2(9))OVT, and binding was measured with quantitative autoradiographic methods. In addition, plasma E2 levels and uterine weights were assessed in animals from each treatment condition. Significant increases in E2-dependent OT receptor binding and uterine weight occurred within 24 h of steroid treatment. After E2 withdrawal, OT receptor binding and uterine weight decreased significantly within 24 h. These results are consistent with the hypothesis that steroid modulation of OT receptor binding is necessary for the induction of sexual receptivity.

  3. Melanocortin Receptor Agonists Facilitate Oxytocin-Dependent Partner Preference Formation in the Prairie Vole.

    PubMed

    Modi, Meera E; Inoue, Kiyoshi; Barrett, Catherine E; Kittelberger, Kara A; Smith, Daniel G; Landgraf, Rainer; Young, Larry J

    2015-07-01

    The central melanocortin (MC) system has been widely studied for its effects on food intake and sexual behavior. However, the MC system, and more specifically the MC4 receptor (MC4R), also interacts with neurochemical systems that regulate socioemotional behaviors, including oxytocin (OT) and dopamine. In monogamous prairie voles, OT and dopamine interact to promote partner preference formation, a laboratory measure of an enduring social bond between mates. Here we investigated the effects of MC receptor activation on partner preference formation in prairie voles, as well as the interaction between the MC and OT systems during this process. Peripheral administration of the brain penetrant MC3/4R receptor peptide agonist, Melanotan II (MTII), and the highly selective, small-molecule MC4R agonist, Pf-446687, enhanced partner preference formation in the prairie vole, but not in the non-monogamous meadow vole. MTII-induced partner preferences were enduring, as they were present 1 week after drug manipulation. The prosocial effects of MCR agonists may be mediated, in part, through modulation of OT, as coadministration of an OT receptor antagonist prevented MTII-induced partner preferences. MTII also selectively activated hypothalamic OT neurons and potentiated central OT release. As OT has been shown to enhance some aspects of social cognition in humans, our data suggest that the MC4R may be a viable therapeutic target for enhancing social function in psychiatric disorders, including autism spectrum disorders and schizophrenia, potentially through activation of the OT system.

  4. The Home-Based Occupational Therapy Intervention in the Alzheimer’s Disease Multiple Intervention Trial (ADMIT)

    PubMed Central

    Schmid, Arlene A.; Spangler-Morris, Carrie; Beauchamp, Rachel C.; Wellington, Miranda C.; Hayden, Whitney M.; Porterfield, Hannah S.; Ferguson, Denisha; Callahan, Christopher M.

    2015-01-01

    There is no way to prevent functional declines related to Alzheimer’s Disease (AD). The use of occupational therapy (OT) has been shown to be successful in managing some aspects of AD. We added home-based OT to evidence-based best practice for AD with the aim of delaying functional decline in people with AD. OT was delivered in the home to a caregiver dyad including the person with AD and her/his caregiver. This paper describes the OT intervention for the AD Multiple Intervention Trial, a parallel randomized controlled trial. We include baseline data on the 180 caregiver dyads. PMID:26997685

  5. Oxytocin and the Development of Parenting in Humans

    PubMed Central

    Gordon, Ilanit; Zagoory-Sharon, Orna; Leckman, James F.; Feldman, Ruth

    2014-01-01

    Background The nonapeptide oxytocin (OT) has been repeatedly implicated in processes of parent-infant bonding in animal models; yet, its role in the development of human parenting has received less attention and no research has addressed the involvement of OT in the transition to fatherhood. Methods Using a prospective longitudinal design, 160 cohabitating mothers and fathers and their firstborn infant were visited at home during the first postpartum weeks and again at 6 months postpartum. Mothers’ and fathers’ plasma OT was analyzed at each time point with enzyme-linked immunosorbent assay methodology. Interactions between each parent and the infant were observed in the postpartum and microcoded for parenting behavior. Results Overall, parental OT increased across the study period and there were no differences between maternal and paternal OT at each time point. Oxytocin showed high intraindividual stability across the first 6 months of parenting and the OT levels of husband and wife were interrelated at both assessments. Maternal OT was related to the amount of affectionate parenting behaviors, including “motherese” vocalizations, the expression of positive affect, and affectionate touch, whereas paternal OT correlated with the degree of stimulatory parenting behaviors, including proprioceptive contact, tactile stimulation, and object presentation. Conclusions Results are the first to describe plasma OT levels in new fathers and mothers across the transition to parenthood in relation to maternal and paternal typical parenting behaviors. These data may provide a normative basis for the study of parenting under conditions of high risk. PMID:20359699

  6. Influence of oxytocin on emotion recognition from body language: A randomized placebo-controlled trial.

    PubMed

    Bernaerts, Sylvie; Berra, Emmely; Wenderoth, Nicole; Alaerts, Kaat

    2016-10-01

    The neuropeptide 'oxytocin' (OT) is known to play a pivotal role in a variety of complex social behaviors by promoting a prosocial attitude and interpersonal bonding. One mechanism by which OT is hypothesized to promote prosocial behavior is by enhancing the processing of socially relevant information from the environment. With the present study, we explored to what extent OT can alter the 'reading' of emotional body language as presented by impoverished biological motion point light displays (PLDs). To do so, a double-blind between-subjects randomized placebo-controlled trial was conducted, assessing performance on a bodily emotion recognition task in healthy adult males before and after a single-dose of intranasal OT (24 IU). Overall, a single-dose of OT administration had a significant effect of medium size on emotion recognition from body language. OT-induced improvements in emotion recognition were not differentially modulated by the emotional valence of the presented stimuli (positive versus negative) and also, the overall tendency to label an observed emotional state as 'happy' (positive) or 'angry' (negative) was not modified by the administration of OT. Albeit moderate, the present findings of OT-induced improvements in bodily emotion recognition from whole-body PLD provide further support for a link between OT and the processing of socio-communicative cues originating from the body of others.

  7. Oxytocin facilitates accurate perception of competition in men and kinship in women.

    PubMed

    Fischer-Shofty, Meytal; Levkovitz, Yechiel; Shamay-Tsoory, Simone G

    2013-03-01

    Despite the dominant role of the hormone oxytocin (OT) in social behavior, little is known about the role of OT in the perception of social relationships. Furthermore, it is unclear whether there are sex differences in the way that OT affects social perception. Here, we employed a double-blind, placebo-controlled crossover design to investigate the effect of OT on accurate social perception. Following treatment, 62 participants completed the Interpersonal Perception Task, a method of assessing the accuracy of social judgments that requires identification of the relationship between people interacting in real life video clips divided into three categories: kinship, intimacy and competition. The findings suggest that OT had a general effect on improving accurate perception of social interactions. Furthermore, we show that OT also involves sex-specific characteristics. An interaction between treatment, task category and sex indicated that OT had a selective effect on improving kinship recognition in women, but not in men, whereas men's performance was improved following OT only for competition recognition. It is concluded that the gender-specific findings reported here may point to some biosocial differences in the effect of OT which may be expressed in women's tendency for communal and familial social behavior as opposed to men's tendency for competitive social behavior. PMID:22446301

  8. 12 CFR 563e.28 - Assigned ratings.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... evidence of discriminatory or other illegal credit practices. (1) The OTS's evaluation of a savings association's CRA performance is adversely affected by evidence of discriminatory or other illegal...

  9. Multiple sites of adaptive plasticity in the owl's auditory localization pathway.

    PubMed

    DeBello, William M; Knudsen, Eric I

    2004-08-01

    In the midbrain auditory localization pathway of the barn owl, a map of auditory space is relayed from the external nucleus of the inferior colliculus (ICX) to the deep and intermediate layers of the optic tectum (OT) and from these layers to the superficial layers. Within the OT, the auditory space map aligns with a visual map of space. Raising young barn owls with a prismatic displacement of the visual field leads to progressive changes in auditory tuning in the OT that tend to realign the auditory space map with the prismatically displaced visual space map. The only known site of this adaptive plasticity is in the ICX, in which the auditory system first creates a map of space. In this study, we identified an additional site of plasticity in the OT. In owls that experienced prisms beginning late in the juvenile period, adaptive shifts in auditory tuning in the superficial layers of the OT exceeded the adaptive shifts that occurred in the deep layers of the OT or in the ICX. Anatomical results from these owls demonstrated that the topography of intrinsic OT connections was systematically altered in the adaptive direction. In juvenile owls, plasticity in the OT increased as plasticity in the ICX decreased. Because plasticity at both sites has been shown to decline substantially in adults, these results suggest that an age-dependent decrease in auditory map plasticity occurs first in the ICX and later at the higher level, in the OT. PMID:15295019

  10. Effects of intranasal oxytocin on neural processing within a socially relevant neural circuit.

    PubMed

    Singh, Fiza; Nunag, Jason; Muldoon, Glennis; Cadenhead, Kristin S; Pineda, Jaime A; Feifel, David

    2016-03-01

    Dysregulation of the Mirror Neuron System (MNS) in schizophrenia (SCZ) may underlie the cognitive and behavioral manifestations of social dysfunction associated with that disorder. In healthy subjects intranasal (IN) oxytocin (OT) improves neural processing in the MNS and is associated with improved social cognition. OT's brain effects can be measured through its modulation of the MNS by suppressing EEG mu-band electrical activity (8-13Hz) in response to motion perception. Although IN OT's effects on social cognition have been tested in SCZ, OT's impact on the MNS has not been evaluated to date. Therefore, we designed a study to investigate the effects of two different OT doses on biological motion-induced mu suppression in SCZ and healthy subjects. EEG recordings were taken after each subject received a single IN administration of placebo, OT-24IU and OT-48IU in randomized order in a double-blind crossover design. The results provide support for OT's regulation of the MNS in both healthy and SCZ subjects, with the optimal dose dependent on diagnostic group and sex of subject. A statistically significant response was seen in SCZ males only, indicating a heightened sensitivity to those effects, although sex hormone related effects cannot be ruled out. In general, OT appears to have positive effects on neural circuitry that supports social cognition and socially adaptive behaviors. PMID:26727038

  11. Day length and sociosexual cohabitation alter central oxytocin receptor binding in female meadow voles (Microtus pennsylvanicus).

    PubMed

    Parker, K J; Phillips, K M; Kinney, L F; Lee, T M

    2001-12-01

    In voles (Microtus), central oxytocin (OT) receptor patterns are associated with interspecific social organization. Social, monogamous voles have more OT receptors in the extended amygdala than asocial, nonmonogamous voles. Nonmonogamous meadow voles (Microtus pennsylvanicus), which exhibit seasonal changes in social organization (long day [LD] females are territorial, short day [SD] females live socially), provide a model for examining whether OT receptor patterns are associated with seasonal changes in intraspecific social behaviors. The authors examined whether sexually inexperienced (naive) SD females had more OT receptor binding than naive LD females. Naive SD females had greater OT receptor binding in the lateral septum (LS), lateral amygdala (LatAmyg), and central amygdala (CenAmyg) than less social, naive LD females. Because both SD and LD females acquire partner preferences, the authors assessed whether OT receptor binding was associated with partner preference onset. For LD females, partner preference onset corresponded with greater OT receptor binding in the anterior olfactory nucleus, LS, and bed nucleus of the stria terminalis, compared with naive LD females. In contrast, naive SD females and those exhibiting partner preferences did not differ. However, SD females that failed to acquire partner preferences showed less OT binding in the LatAmyg and CenAmyg. This study is the first to show that central OT receptor patterns are associated with seasonal changes in intraspecific social organization and partner preference onset in a nonmonogamous rodent.

  12. Influence of oxytocin on emotion recognition from body language: A randomized placebo-controlled trial.

    PubMed

    Bernaerts, Sylvie; Berra, Emmely; Wenderoth, Nicole; Alaerts, Kaat

    2016-10-01

    The neuropeptide 'oxytocin' (OT) is known to play a pivotal role in a variety of complex social behaviors by promoting a prosocial attitude and interpersonal bonding. One mechanism by which OT is hypothesized to promote prosocial behavior is by enhancing the processing of socially relevant information from the environment. With the present study, we explored to what extent OT can alter the 'reading' of emotional body language as presented by impoverished biological motion point light displays (PLDs). To do so, a double-blind between-subjects randomized placebo-controlled trial was conducted, assessing performance on a bodily emotion recognition task in healthy adult males before and after a single-dose of intranasal OT (24 IU). Overall, a single-dose of OT administration had a significant effect of medium size on emotion recognition from body language. OT-induced improvements in emotion recognition were not differentially modulated by the emotional valence of the presented stimuli (positive versus negative) and also, the overall tendency to label an observed emotional state as 'happy' (positive) or 'angry' (negative) was not modified by the administration of OT. Albeit moderate, the present findings of OT-induced improvements in bodily emotion recognition from whole-body PLD provide further support for a link between OT and the processing of socio-communicative cues originating from the body of others. PMID:27442997

  13. Melanocortin Receptor Agonists Facilitate Oxytocin-Dependent Partner Preference Formation in the Prairie Vole

    PubMed Central

    Modi, Meera E; Inoue, Kiyoshi; Barrett, Catherine E; Kittelberger, Kara A; Smith, Daniel G; Landgraf, Rainer; Young, Larry J

    2015-01-01

    The central melanocortin (MC) system has been widely studied for its effects on food intake and sexual behavior. However, the MC system, and more specifically the MC4 receptor (MC4R), also interacts with neurochemical systems that regulate socioemotional behaviors, including oxytocin (OT) and dopamine. In monogamous prairie voles, OT and dopamine interact to promote partner preference formation, a laboratory measure of an enduring social bond between mates. Here we investigated the effects of MC receptor activation on partner preference formation in prairie voles, as well as the interaction between the MC and OT systems during this process. Peripheral administration of the brain penetrant MC3/4R receptor peptide agonist, Melanotan II (MTII), and the highly selective, small-molecule MC4R agonist, Pf-446687, enhanced partner preference formation in the prairie vole, but not in the non-monogamous meadow vole. MTII-induced partner preferences were enduring, as they were present 1 week after drug manipulation. The prosocial effects of MCR agonists may be mediated, in part, through modulation of OT, as coadministration of an OT receptor antagonist prevented MTII-induced partner preferences. MTII also selectively activated hypothalamic OT neurons and potentiated central OT release. As OT has been shown to enhance some aspects of social cognition in humans, our data suggest that the MC4R may be a viable therapeutic target for enhancing social function in psychiatric disorders, including autism spectrum disorders and schizophrenia, potentially through activation of the OT system. PMID:25652247

  14. 12 CFR 517.1 - Purpose and scope.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... pertain to services in support of OTS's business operations, such as consulting, programming, auditing, expert witnesses, customized training, relocation services, information systems technology...

  15. 12 CFR 517.1 - Purpose and scope.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... pertain to services in support of OTS's business operations, such as consulting, programming, auditing, expert witnesses, customized training, relocation services, information systems technology...

  16. 12 CFR 517.1 - Purpose and scope.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... pertain to services in support of OTS's business operations, such as consulting, programming, auditing, expert witnesses, customized training, relocation services, information systems technology...

  17. A common oxytocin receptor gene (OXTR) polymorphism modulates intranasal oxytocin effects on the neural response to social cooperation in humans

    PubMed Central

    Feng, Chunliang; Lori, Adriana; Waldman, Irwin D.; Binder, Elisabeth B.; Haroon, Ebrahim; Rilling, James K.

    2015-01-01

    Intranasal oxytocin (OT) can modulate social-emotional functioning and related brain activity in humans. Consequently, OT has been discussed as a potential treatment for psychiatric disorders involving social behavioral deficits. However, OT effects are often heterogeneous across individuals. Here we explore individual differences in OT effects on the neural response to social cooperation as a function of the rs53576 polymorphism of the oxytocin receptor gene (OXTR). Previously, we conducted a double-blind, placebo-controlled study in which healthy men and women were randomized to treatment with intranasal OT or placebo. Afterwards, they were imaged with fMRI while playing an iterated Prisoner’s Dilemma Game with same-sex partners. Within the left ventral caudate nucleus, intranasal OT treatment increased activation to reciprocated cooperation in men, but tended to decrease activation in women. Here, we show that these sex differences in OT effects are specific to individuals with the rs53576 GG genotype, and are not found for other genotypes (rs53576 AA/AG). Thus, OT may increase the reward or salience of positive social interactions for male GG homozygotes, while decreasing those processes for female GG homozygotes. These results suggest that rs53576 genotype is an important variable to consider in future investigations of the clinical efficacy of intranasal OT treatment. PMID:26178189

  18. Sniff and mimic - Intranasal oxytocin increases facial mimicry in a sample of men.

    PubMed

    Korb, Sebastian; Malsert, Jennifer; Strathearn, Lane; Vuilleumier, Patrik; Niedenthal, Paula

    2016-08-01

    The neuropeptide oxytocin (OT) has many potential social benefits. For example, intranasal administration of OT appears to trigger caregiving behavior and to improve the recognition of emotional facial expressions. But the mechanism for these effects is not yet clear. Recent findings relating OT to action imitation and to the visual processing of the eye region of faces point to mimicry as a mechanism through which OT improves processing of emotional expression. To test the hypothesis that increased levels of OT in the brain enhance facial mimicry, 60 healthy male participants were administered, in a double-blind between-subjects design, 24 international units (IUs) of OT or placebo (PLA) through nasal spray. Facial mimicry and emotion judgments were recorded in response to movie clips depicting changing facial expressions. As expected, facial mimicry was increased in the OT group, but effects were strongest for angry infant faces. These findings provide further evidence for the importance of OT in social cognitive skills, and suggest that facial mimicry mediates the effects of OT on improved emotion recognition.

  19. Oxytocin facilitates fidelity in well-established marmoset pairs by reducing sociosexual behavior toward opposite-sex strangers

    PubMed Central

    Cavanaugh, Jon; Mustoe, Aaryn C.; Taylor, Jack H.; French, Jeffrey A.

    2014-01-01

    Summary Behavioral strategies that facilitate the maintenance of social bonds are critical for the preservation of high-quality social relationships. Central oxytocin (OT) activity modulates the behavioral features of socially monogamous relationships in a number of mammalian species (including marmoset monkeys), and plays a vital role in the behavioral maintenance of long-term social relationships. Two distinct variants of OT have been identified in some New World primates (including marmosets; Lee et al., 2011). The marmoset variant of the oxytocin ligand (Pro8-OT) is structurally distinct from the consensus mammalian variant of the oxytocin ligand (Leu8-OT), due to a proline substitution at the 8th amino-acid position. The goal of the present study was to determine if treating marmosets with Pro8-OT, relative to treatments with Leu8-OT, control saline, or an OT antagonist, had modulatory effects on the behavioral maintenance of long-term social relationships in marmosets. Treatment with the Pro8 variant, but not the Leu8 variant, of OT facilitated fidelity with a long-term partner by reducing time spent in close proximity with an opposite-sex stranger. However, this facilitative effect of Pro8-OT on proximity behavior manifested itself differently in male and female marmosets, such that females preferred to interact socially with their partner rather than a stranger when treated with Pro8-OT, while males spent less time in close proximity with both their partner and a stranger when treated with Pro8-OT. Furthermore, treatment with Pro8-OT, but not Leu8-OT, significantly delayed the expression of sexual solicitation behavior toward an opposite-sex stranger in both male and female marmosets, but had no effect on sociosexual behavior directed toward a long-term partner. These results suggest that the OT system is highly involved in reducing fidelity-threatening behaviors in well-established marmoset pairs, and that the effects were only produced by species

  20. Intranasal oxytocin effects on social cognition: a critique

    PubMed Central

    Evans, Simon L.; Monte, Olga Dal; Noble, Pamela; Averbeck, Bruno B.

    2014-01-01

    The last decade has seen a large number of published findings supporting the hypothesis that intranasally delivered oxytocin (OT) can enhance the processing of social stimuli and regulate social emotion-related behaviors such as trust, memory, fidelity, and anxiety. The use of nasal spray for administering OT in behavioral research has become a standard method, but many questions still exist regarding its action. OT is a peptide that cannot cross the blood-brain barrier, and it has yet to be shown that it does indeed reach the brain when delivered intranasally. Given the evidence, it seems highly likely that OT does affect behavior when delivered as a nasal spray. These effects may be driven by at least three possible mechanisms. First, the intranasally delivered OT may diffuse directly into the CNS where it directly engages OT receptors. Second, the intranasally delivered OT may trigger increased central release via an indirect peripheral mechanism. And third, the indirect peripheral effects may directly lead to behavioral effects via some mechanism other than increased central release. Although intranasally delivered OT likely affects behavior, there are conflicting reports as to the exact nature of those behavioral changes: some studies suggest that OT effects are not always “pro-social” and others suggest effects on social behaviors are due to a more general anxiolytic effect. In this critique, we draw from work in healthy human populations and the animal literature to review the mechanistic aspects of intranasal OT delivery, and to discuss intranasal OT effects on social cognition and behavior. We conclude that future work should control carefully for anxiolytic and gender effects, which could underlie inconsistencies in the existing literature. PMID:24239931

  1. Association of oxytocin level and less severe forms of childhood maltreatment history among healthy Japanese adults involved with child care

    PubMed Central

    Mizuki, Rie; Fujiwara, Takeo

    2015-01-01

    Background: Oxytocin (OT) is known to play a role in stress regulation. The association between childhood maltreatment history and neuropeptide OT concentration is inconsistent due to the varying degrees of severity of childhood maltreatment, among other contributing factors. Less severe forms of childhood maltreatment history might enhance OT concentrations as a response to coping with social stress within the family. The purpose of this study is to investigate the association between less severe forms of childhood maltreatment history and OT concentrations among healthy adults. Method: Eighty adults (49 women and 31 men) with 18- to 48-month-old children were recruited using a snowball sample in Tokyo, Japan. Urine samples were collected for OT measurement. Less severe (low and moderate) childhood maltreatment history, including physical abuse, physical neglect, emotional abuse, emotional neglect, and sexual abuse, was assessed using the self-report questionnaire, the Childhood Trauma Questionnaire. Results: Less severe physical abuse was significantly associated with higher OT concentration after adjusting for age (p = 0.014). Also, less severe forms of physical abuse were independently significantly associated with higher OT concentration after controlling for other types of childhood maltreatment (p = 0.027). A positive dose-response association between the number of less severe childhood maltreatment types and OT concentration was observed (p = 0.031). Conclusion: A history of less severe forms of childhood physical abuse was associated with higher OT concentration in healthy adults. Poly-victimization of several types of less severe childhood maltreatment was also associated with higher OT concentrations. Less severe forms of childhood maltreatment might enhance OT concentrations in order to cope with social stress. PMID:26157369

  2. Molecular, immunohistochemical, and pharmacological evidence of oxytocin's role as inhibitor of carbohydrate but not fat intake.

    PubMed

    Olszewski, Pawel K; Klockars, Anica; Olszewska, Agnieszka M; Fredriksson, Robert; Schiöth, Helgi B; Levine, Allen S

    2010-10-01

    Oxytocin (OT) facilitates feeding termination stemming from high osmolality, stomach distention, and malaise. Recent knockout (KO) studies suggested a crucial function for OT in carbohydrate intake: OT-/- mice had increased preference for carbohydrates, including sucrose, but not fat (Intralipid). In striking contrast, sugar appetite was unaffected in the OT receptor KO mouse; data from wild-type animals have been insufficient. Therefore, we examined the involvement of OT in the regulation of sucrose vs. fat intake in C57BL/6 mice that served as a background KO strain. We exposed mice to a meal of sucrose or Intralipid and determined that the percentage of c-Fos-immunoreactive paraventricular hypothalamic OT neurons was elevated at termination of intake of either of the tastants, but this increase was 2-fold higher in sucrose-fed mice. A 48-h exposure to sucrose compared with Intralipid caused up-regulation of OT mRNA, whereas inherent individual preferences for sucrose vs. fat were not associated with differences in baseline OT expression as established with quantitative PCR. We found that L-368,899, an OT receptor antagonist, increased sugar intake when sucrose was presented alone or concurrently with Intralipid; it had no effect on Intralipid or total calorie consumption. L-368,899 affected Fos immunoreactivity in the paraventricular hypothalamus, arcuate nucleus, amygdala, and nucleus of the solitary tract, areas involved in aversion, satiety, and reward. This pattern serves as neuroanatomical basis of OT's complex role in food intake, including sucrose intake. The current findings expand our knowledge on OT and suggest that it acts as a carbohydrate-specific inhibitor of feeding.

  3. Blockade of NK3R signaling in the PVN decreases vasopressin and oxytocin release and c-Fos expression in the magnocellular neurons in response to hypotension.

    PubMed

    Haley, Gwendolen E; Flynn, Francis W

    2008-10-01

    Tachykinin neurokinin 3 receptor (NK3R) signaling has a broad role in vasopressin (VP) and oxytocin (OT) release. Hydralazine (HDZ)-induced hypotension activates NK3R expressed by magnocellular neurons, increases plasma VP and OT levels, and induces c-Fos expression in VP and OT neurons. Intraventricular pretreatment with the specific NK3R antagonist, SB-222200, eliminates the HDZ-stimulated VP and OT release. NK3R are distributed in the central pathways conveying hypotension information to the magnocellular neurons, and the NK3R antagonist could act anywhere in the pathways. Alternatively, the antagonist could act at the NK3R expressed by the magnocellular neurons. To determine whether blockade of NK3R on magnocellular neurons impairs VP and OT release to HDZ, rats were pretreated with a unilateral PVN injection of 0.15 M NaCl or SB-222200 prior to an intravenous injection of 0.15 M NaCl or HDZ. Blood samples were taken, and brains were processed for VP/c-Fos and OT/c-Fos immunohistochemistry. Intravenous injection of 0.15 M NaCl did not alter plasma hormone levels, and little c-Fos immunoreactivity was present in the PVN. Conversely, intravenous injection of HDZ increased plasma VP and OT levels and c-Fos expression in VP and OT magnocellular neurons. Intra-PVN injection of SB-222200 prior to an intravenous injection of HDZ significantly decreased c-Fos expression in both VP and OT neurons by approximately 70% and attenuated plasma VP and OT levels by 33% and 35%, respectively. Therefore, NK3R signaling in magnocellular neurons has a critical role for the release of VP and OT in response to hypotension.

  4. Adolescent pre-treatment with oxytocin protects against adult methamphetamine-seeking behavior in female rats.

    PubMed

    Hicks, Callum; Cornish, Jennifer L; Baracz, Sarah J; Suraev, Anastasia; McGregor, Iain S

    2016-03-01

    The neuropeptide oxytocin (OT), given acutely, reduces self-administration of the psychostimulant drug methamphetamine (METH). Additionally, chronic OT administration to adolescent rats reduces levels of alcohol consumption in adulthood, suggesting developmental neuroplasticity in the OT system relevant to addiction-related behaviors. Here, we examined whether OT exposure during adolescence might subsequently inhibit METH self-administration in adulthood. Female Sprague-Dawley rats were administered vehicle or OT (1 mg/kg, i.p.) once daily from postnatal days (PND) 28 to 37 (adolescence). At PND 62 (adulthood), rats were trained to self-administer METH (intravenous, i.v.) in daily 2-hour sessions for 10 days under a fixed ratio 1 (FR1) reinforcement schedule, followed by determination of dose-response functions (0.01-0.3 mg/kg/infusion, i.v.) under both FR1 and progressive ratio (PR) schedules of reinforcement. Responding was then extinguished, and relapse to METH-seeking behavior assessed following priming doses of non-contingent METH (0.1-1 mg/kg, i.p.). Finally, plasma was collected to determine pre-treatment effects on OT and corticosterone levels. Results showed that OT pre-treatment did not significantly inhibit the acquisition of METH self-administration or FR1 responding. However, rats pre-treated with OT responded significantly less for METH under a PR reinforcement schedule, and showed reduced METH-primed reinstatement with the 1 mg/kg prime. Plasma OT levels were also significantly higher in OT pre-treated rats. These results confirm earlier observations that adolescent OT exposure can subtly, yet significantly, inhibit addiction-relevant behaviors in adulthood.

  5. The effect of oxytocin on the anthropomorphism of touch.

    PubMed

    Peled-Avron, Leehe; Perry, Anat; Shamay-Tsoory, Simone G

    2016-04-01

    One of the leading hypotheses regarding the mechanism underlying the social effects of oxytocin (OT) is the "social salience hypothesis", which proposes that OT alters the attentional salience of social cues in a context-dependent manner. Recently, OT was implicated in the process of anthropomorphism; specifically, OT was found to increase the tendency to ascribe social meaning to inanimate stimuli. However, the precise component of social interaction that contributes to this effect remains unclear. Because OT plays a role in the response to touch, whether or not objects are touching in a social context may represent the prominent trigger. Given that OT plays a major role in both anthropomorphism and touch, it is reasonable to assume that OT enhances anthropomorphism specifically for non-human touch, further clarifying its role in altering the perceptual salience of social cues. Here, we examined whether intranasal delivery of OT influences anthropomorphism for touch in inanimate objects. To that end, we implicitly measured the emotional reactions of participants (N=51) to photos that depicted two humans or two inanimate objects either touching or not touching. We asked them to rate whether they will include each photo in an emotional album and found that OT treatment increased the likelihood of inclusion in an emotional album to photos that contain touch, particularly between inanimate objects. In a follow-up experiment we found that the more human the inanimate objects were perceived, the more included they were in the emotional album. Our findings demonstrate that OT can enhance the social meaning of touch between two inanimate objects and advance our understanding of the mechanisms underlying the ability of OT to anthropomorphize environmental cues. PMID:26827294

  6. Oxytocin regulates gastrointestinal motility, inflammation, macromolecular permeability, and mucosal maintenance in mice

    PubMed Central

    Margolis, Kara G.; Li, Zhishan; Gershon, Michael D.

    2014-01-01

    Enteric neurons express oxytocin (OT); moreover, enteric neurons and enterocytes express developmentally regulated OT receptors (OTRs). Although OT (with secretin) opposes intestinal inflammation, physiological roles played by enteric OT/OTR signaling have not previously been determined. We tested hypotheses that OT/OTR signaling contributes to enteric nervous system (ENS)-related gastrointestinal (GI) physiology. GI functions and OT effects were compared in OTR-knockout (OTRKO) and wild-type (WT) mice. Stool mass and water content were greater in OTRKO mice than in WT. GI transit time in OTRKO animals was faster than in WT; OT inhibited in vitro generation of ENS-dependent colonic migrating motor complexes in WT but not in OTRKO mice. Myenteric neurons were hyperplastic in OTRKO animals, and mucosal exposure to cholera toxin (CTX) in vitro activated Fos in more myenteric neurons in OTRKO than WT than in WT mice; OT inhibited the CTX response in WT but not in OTRKO mice. Villi and crypts were shorter in OTRKO than in WT mice, and transit-amplifying cell proliferation in OTRKO crypts was deficient. Macromolecular intestinal permeability in OTRKO was greater than WT mice, and experimental colitis was more severe in OTRKO mice; moreover, OT protected WT animals from colitis. Observations suggest that OT/OTR signaling acts as a brake on intestinal motility, decreases mucosal activation of enteric neurons, and promotes enteric neuronal development and/or survival. It also regulates proliferation of crypt cells and mucosal permeability; moreover OT/OTR signaling is protective against inflammation. Oxytocinergic signaling thus appears to play an important role in multiple GI functions that are subject to neuronal regulation. PMID:25147234

  7. Mitochondrial Bioenergetics of Metastatic Breast Cancer Cells in Response to Dynamic Changes in Oxygen Tension: Effects of HIF-1α

    PubMed Central

    Oliva, Claudia R.; Griguer, Corinne E.; Darley-Usmar, Victor; Hurst, Douglas R.; Welch, Danny R.; Landar, Aimee

    2013-01-01

    Solid tumors are characterized by regions of low oxygen tension (OT), which play a central role in tumor progression and resistance to therapy. Low OT affects mitochondrial function and for the cells to survive, mitochondria must functionally adapt to low OT to maintain the cellular bioenergetics. In this study, a novel experimental approach was developed to examine the real-time bioenergetic changes in breast cancer cells (BCCs) during adaptation to OT (from 20% to <1% oxygen) using sensitive extracellular flux technology. Oxygen was gradually removed from the medium, and the bioenergetics of metastatic BCCs (MDA-MB-231 and MCF10CA clones) was compared with non-tumorigenic (MCF10A) cells. BCCs, but not MCF10A, rapidly responded to low OT by stabilizing HIF-1α and increasing HIF-1α responsive gene expression and glucose uptake. BCCs also increased extracellular acidification rate (ECAR), which was markedly lower in MCF10A. Interestingly, BCCs exhibited a biphasic response in basal respiration as the OT was reduced from 20% to <1%. The initial stimulation of oxygen consumption is found to be due to increased mitochondrial respiration. This effect was HIF-1α-dependent, as silencing HIF-1α abolished the biphasic response. During hypoxia and reoxygenation, BCCs also maintained oxygen consumption rates at specific OT; however, HIF-1α silenced BCC were less responsive to changes in OT. Our results suggest that HIF-1α provides a high degree of bioenergetic flexibility under different OT which may confer an adaptive advantage for BCC survival in the tumor microenvironment and during invasion and metastasis. This study thus provides direct evidence for the cross-talk between HIF-1α and mitochondria during adaptation to low OT by BCCs and may be useful in identifying novel therapeutic agents that target the bioenergetics of BCCs in response to low OT. PMID:23840849

  8. Long-term exposure to intranasal oxytocin in a mouse autism model.

    PubMed

    Bales, K L; Solomon, M; Jacob, S; Crawley, J N; Silverman, J L; Larke, R H; Sahagun, E; Puhger, K R; Pride, M C; Mendoza, S P

    2014-01-01

    Oxytocin (OT) is a neuropeptide involved in mammalian social behavior. It is currently in clinical trials for the treatment of autism spectrum disorder (ASD). Previous studies in healthy rodents (prairie voles and C57BL/6J mice) have shown that there may be detrimental effects of long-term intranasal administration, raising the questions about safety and efficacy. To investigate the effects of OT on the aspects of ASD phenotype, we conducted the first study of chronic intranasal OT in a well-validated mouse model of autism, the BTBR T+ Itpr3tf/J inbred strain (BTBR), which displays low sociability and high repetitive behaviors. BTBR and C57BL/6J (B6) mice (N=94) were administered 0.8  IU/kg of OT intranasally, daily for 30 days, starting on day 21. We ran a well-characterized set of behavioral tasks relevant to diagnostic and associated symptoms of autism, including juvenile reciprocal social interactions, three-chambered social approach, open-field exploratory activity, repetitive self-grooming and fear-conditioned learning and memory, some during and some post treatment. Intranasal OT did not improve autism-relevant behaviors in BTBR, except for female sniffing in the three-chambered social interaction test. Male saline-treated BTBR mice showed increased interest in a novel mouse, both in chamber time and sniffing time, whereas OT-treated male BTBR mice showed a preference for the novel mouse in sniffing time only. No deleterious effects of OT were detected in either B6 or BTBR mice, except possibly for the lack of a preference for the novel mouse's chamber in OT-treated male BTBR mice. These results highlight the complexity inherent in understanding the effects of OT on behavior. Future investigations of chronic intranasal OT should include a wider dose range and early developmental time points in both healthy rodents and ASD models to affirm the efficacy and safety of OT. PMID:25386957

  9. Long-term exposure to intranasal oxytocin in a mouse autism model

    PubMed Central

    Bales, K L; Solomon, M; Jacob, S; Crawley, J N; Silverman, J L; Larke, R H; Sahagun, E; Puhger, K R; Pride, M C; Mendoza, S P

    2014-01-01

    Oxytocin (OT) is a neuropeptide involved in mammalian social behavior. It is currently in clinical trials for the treatment of autism spectrum disorder (ASD). Previous studies in healthy rodents (prairie voles and C57BL/6J mice) have shown that there may be detrimental effects of long-term intranasal administration, raising the questions about safety and efficacy. To investigate the effects of OT on the aspects of ASD phenotype, we conducted the first study of chronic intranasal OT in a well-validated mouse model of autism, the BTBR T+ Itpr3tf/J inbred strain (BTBR), which displays low sociability and high repetitive behaviors. BTBR and C57BL/6J (B6) mice (N=94) were administered 0.8  IU/kg of OT intranasally, daily for 30 days, starting on day 21. We ran a well-characterized set of behavioral tasks relevant to diagnostic and associated symptoms of autism, including juvenile reciprocal social interactions, three-chambered social approach, open-field exploratory activity, repetitive self-grooming and fear-conditioned learning and memory, some during and some post treatment. Intranasal OT did not improve autism-relevant behaviors in BTBR, except for female sniffing in the three-chambered social interaction test. Male saline-treated BTBR mice showed increased interest in a novel mouse, both in chamber time and sniffing time, whereas OT-treated male BTBR mice showed a preference for the novel mouse in sniffing time only. No deleterious effects of OT were detected in either B6 or BTBR mice, except possibly for the lack of a preference for the novel mouse's chamber in OT-treated male BTBR mice. These results highlight the complexity inherent in understanding the effects of OT on behavior. Future investigations of chronic intranasal OT should include a wider dose range and early developmental time points in both healthy rodents and ASD models to affirm the efficacy and safety of OT. PMID:25386957

  10. Comparison between Observed Tsunami Heights and Numerical Simulation of the 1854 Ansei-Tokai Earthquake Tsunami in Gokasho Bay, central Japan

    NASA Astrophysics Data System (ADS)

    Naruhashi, R.; Satake, K.; Heidarzadeh, M.; Harada, T.

    2014-12-01

     Gokasho Bay is a blockade inner bay which has typical ria coasts and drowned valleys. It is located in the central Kii Peninsula and faces the Nankai Trough subduction zone. This Kumano-nada coastal area has been repeatedly striked by historical great tsunamis. For the 1854 Ansei-Tokai earthquake and its tsunami, there are comparatively many historical records including historical documents and oral traditions for tsunami behavior and damages along the coast. Based on these records, a total of 42 tsunami heights were measured by using a laser range finder and a hand level on the basis of spot elevation given by 1/2500 topographical maps. The average inundation height of whole bay area was approximately 4 - 5 m. On the whole, in the closed-off section of the bay, large values were obtained. For example, the average value in Gokasho-ura town area was 4 m, and the maximum run-up height along the Gokasho river was 6.8 m. Particularly in Konsa, located in the most closed-off section of the bay, tsunami heights ranged between 4 - 11 m, and were higher than those in other districts. It was comparatively high along the eastern coast and eastern baymouth. We simulate the distribution of the tsunami wave heights using numerical modeling, and compare the simulation results and above-mentioned actual historical data and results of our field survey. Based on fault models by Ando (1975), Aida (1981), and Annaka et al. (2003), the tsunami simulation was performed. After comparing the calculated results by three fault models, the wave height based on the model by Annaka et al. (2003) was found to have better agreement with observations. Moreover, the wave height values in a closed-off section of bay and at the eastern baymouth are high consistent with our survey data.

  11. Advancing Ergonomic Expertise in Occupational Therapists: Using an Online Course to Facilitate Knowledge Acquisition and Application of Ergonomics in Daily Practice

    ERIC Educational Resources Information Center

    Finch, Denise E.

    2012-01-01

    Health promotion, prevention of disease and disability, and enhancing participation in meaningful life activities are areas that occupational therapists (OT) can contribute to at the individual, organizational and population levels (AOTA, 2008; Scaffa, Van Slyke & Brownson, 2008). OTs that are skilled in ergonomic assessment and evidence-based…

  12. Molecular evolution of the neurohypophysial hormone precursors in mammals: Comparative genomics reveals novel mammalian oxytocin and vasopressin analogues.

    PubMed

    Wallis, Michael

    2012-11-01

    Among vertebrates the neurohypophysial hormones show considerable variation. However, in eutherian mammals they have been considered rather conserved, with arginine vasopressin (AVP) and oxytocin (OT) in all species except pig and some relatives, where lysine vasopressin replaces AVP. The availability of genomic data for a wide range of mammals makes it possible to assess whether these peptides and their precursors may be more variable in Eutheria than previously suspected. A survey of these data confirms that AVP and OT occur in most eutherians, but with exceptions. In a New-World monkey (marmoset, Callithrix jacchus) and in tree shrew (Tupaia belangeri), Pro(8)OT replaces OT, confirming a recent report for these species. In armadillo (Dasypus novemcinctus) Leu(3)OT replaces OT, while in tenrec (Echinops telfairi) Thr(4)AVP replaces AVP. In these two species there is also evidence for additional genes/pseudogenes, encoding much-modified forms of AVP, but in most other eutherian species there is no evidence for additional neurohypophysial hormone genes. Evolutionary analysis shows that sequences of eutherian neurohypophysial hormone precursors are generally strongly conserved, particularly those regions encoding active peptide and neurophysin. The close association between OT and VP genes has led to frequent gene conversion of sequences encoding neurophysins. A monotreme, platypus (Ornithorhynchus anatinus) has genes for OT and AVP, organized tail-to-tail as in eutherians, but in marsupials 3-4 genes are present for neurohypophysial hormones, organized tail-to-head as in lower vertebrates.

  13. Intranasal oxytocin increases social grooming and food sharing in the common vampire bat Desmodus rotundus.

    PubMed

    Carter, Gerald G; Wilkinson, Gerald S

    2015-09-01

    Intranasal oxytocin (OT) delivery has been used to non-invasively manipulate mammalian cooperative behavior. Such manipulations can potentially provide insight into both shared and species-specific mechanisms underlying cooperation. Vampire bats are remarkable for their high rates of allogrooming and the presence of regurgitated food sharing among adults. We administered intranasal OT to highly familiar captive vampire bats of varying relatedness to test for an effect on allogrooming and food sharing. We found that intranasal OT did not have a detectable effect on food-sharing occurrence, but it did increase the size of regurgitated food donations when controlling for dyad and amount of allogrooming. Intranasal OT in females increased the amount of allogrooming per partner and across all partners per trial, but not the number of partners. We also found that the peak effect of OT treatments occurred 30-50min after administration, which is consistent with the reported latency for intranasal OT to affect relevant brain areas in rats and mice. Our results suggest that intranasal OT is a potential tool for influencing dyadic cooperative investments, but measuring prior social relationships may be necessary to interpret the results of hormonal manipulations of cooperative behavior and it may be difficult to alter partner choice in vampire bats using intranasal OT alone.

  14. Duplicated Clock genes with unique polyglutamine domains provide evidence for nonhomologous recombination in Chinook salmon (Oncorhynchus tshawytscha).

    PubMed

    O'Malley, K G; Banks, M A

    2008-01-01

    Circadian rhythms underlie diverse life functions ranging from cellular activities to behavior. Multiple clock genes play a central role in the generation of these rhythms. We partially characterized two copies of the Clock gene from Chinook salmon (Oncorhynchus tshawytscha), OtsClock1a and OtsClock1b. The 6,460 bp OtsClock1a sequence contains 16 exons, 15 introns and encompasses three highly conserved domains indicating it is a novel member of the bHLH-PAS superfamily of transcription factors. The second copy, OtsClock1b, consists of five exons and five introns spanning 1,945 bp. A polyglutamine repeat motif (PolyQ), characteristic of a majority of CLOCK proteins, is present in both OTSCLOCK1a and OTSCLOCK1b. However, the Chinook PolyQ domains are uniquely positioned inside the gene. Interestingly, a 1,200 bp non-coding segment located downstream of the OtsClock1a PolyQ domain is absent from OtsClock1b. This insertion/deletion is 91% similar to the Salmo salar Transferrin gene. A phylogenetic analysis of 11 CLOCK proteins shows that OtsClock1a and OtsClock1b are paralogs which likely arose subsequent to the salmonid genome-wide duplication event. Ultimately, the Chinook salmon Clock genes are key components to our understanding the genetic mechanisms underlying temporally regulated life history traits in Pacific salmonids. PMID:17503191

  15. Open letter to Minister Helen Morton.

    PubMed

    Campbell, Emma; Oke, Lin; Cairney, Joanne; Fergusson, Fiona; Booth, Jodie; Taylor, Karen; Baird, Tyson; Sheppard, Loretta; Jensen, Heather; Kao, Kevin

    2015-10-01

    This letter was sent to the Honourable Helen Morton by Emma Campbell after the Occupational Therapy Australia National Conference. A number of members of First Australian and Australian OTs online were keen to show their support for Emma's letter and share this with other OT colleagues. PMID:26726327

  16. Rhythmic secretion of prolactin in rats: action of oxytocin coordinated by vasoactive intestinal polypeptide of suprachiasmatic nucleus origin.

    PubMed

    Egli, Marcel; Bertram, Richard; Sellix, Michael T; Freeman, Marc E

    2004-07-01

    Prolactin (PRL) is secreted from lactotrophs of the anterior pituitary gland of rats in a unique pattern in response to uterine cervical stimulation (CS) during mating. Surges of PRL secretion occur in response to relief from hypothalamic dopaminergic inhibition and stimulation by hypothalamic releasing neurohormones. In this study, we characterized the role of oxytocin (OT) in this system and the involvement of vasoactive intestinal polypeptide (VIP) from the suprachiasmatic nucleus (SCN) in controlling OT and PRL secretion of CS rats. The effect of OT on PRL secretion was demonstrated in cultured lactotrophs showing simultaneous enhanced secretion rate and increased intracellular Ca(2+). Neurosecretory OT cells of the hypothalamic paraventricular nucleus that express VIP receptors were identified by using immunocytochemical techniques in combination with the retrogradely transported neuronal tracer Fluoro-Gold (iv injected). OT measurements of serial blood samples obtained from ovariectomized (OVX) CS rats displayed a prominent increase at the time of the afternoon PRL peak. The injection of VIP antisense oligonucleotides into the SCN abolished the afternoon increase of OT and PRL in CS-OVX animals. These findings suggest that VIP from the SCN contributes to the regulation of OT and PRL secretion in CS rats. We propose that in CS rats the regulatory mechanism(s) for PRL secretion comprise coordinated action of neuroendocrine dopaminergic and OT cells, both governed by the daily rhythm of VIP-ergic output from the SCN. This hypothesis is illustrated with a mathematical model. PMID:15033917

  17. [Interdisciplinary teamwork in the OR: Identification and measurement of teamwork in the operating room].

    PubMed

    Passauer-Baierl, Stefanie; Baschnegger, Heiko; Bruns, Christiane; Weigl, Matthias

    2014-01-01

    Effective teamwork is one of the essentials in conducting successful and safe surgical procedures in the operating theatre (OT). The present paper aims to provide a selective review of various approaches describing effective interdisciplinary teamwork in the OT. Furthermore, it covers observational methods to assess OT teamwork with particular focus on Germany. Our definition of successful surgical teamwork is based on an already established classification system considering five criteria for effective and safe OT teams: coordination, communication, cooperation, leadership, and team monitoring. Well-defined and reliable measures are necessary to examine the quality of OT teamwork. Those methods should entail the special characteristics of the OT team. They should include all phases of the surgical procedure and incorporate all the professions involved (surgeons, surgical nurses, and anaesthetic staff). We conclude that research into methods for the assessment of OTs in Germany needs to be undertaken as a prerequisite to investigating the relationship between OT teamwork and its effects on patient safety and surgical quality.

  18. MASTER discoveries: 2 new bright dwarf novae

    NASA Astrophysics Data System (ADS)

    Shumkov, V.; Gress, O.; Lipunov, V.; Buckley, D.; Rebolo, R.; Serra-Ricart, M.; Tiurina, N.; Balanutsa, P.; Kuznetsov, A.; Gorbovskoy, E.; Kornilov, V.; Chazov, V.; Vlasenko, D.; Vladimirov, V.; Ivanov, K.; Potter, S.

    2016-10-01

    MASTER-IAC auto-detection system (Lipunov et al., 2010, Advances in Astronomy, vol. 2010, 30L) discovered OT source at (RA, Dec) = 05h 31m 41.25s -14d 11m 59.0s on 2016-10-23.20152 UT. The OT unfiltered magnitude is 16.0m (limit 18.8m).

  19. MASTER: optical transients

    NASA Astrophysics Data System (ADS)

    Gress, O.; Balanutsa, P.; Shumkov, V.; Lipunov, V.; Buckley, D.; Rebolo, R.; Ricart, M. Serra; Podesta, R.; Levato, H.; Gorbovskoy, E.; Tiurina, N.; Kuznetsov, A.; Kornilov, V.; Pogrosheva, T.; Chazov, V.; Vladimirov, V.; Vlasenko, D.; Potter, S.; Lopez, C.; Podesta, F.; Saffe, C.

    2016-10-01

    MASTER-SAAO auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 14h 38m 49.60s -44d 37m 24.5s on 2016-10-01.73438 UT with unfiltered m_OT=16.4m (mlim=19.7m).

  20. MASTER discoveries: bright PSN, new dwarf novae and QSO flare

    NASA Astrophysics Data System (ADS)

    Shumkov, V.; Balanutsa, P.; Gress, O.; Popova, E.; Lipunov, V.; Buckley, D.; Rebolo, R.; Serra-Ricart, M.; Tlatov, A.; Budnev, N.; Ivanov, K.; Podesta, R.; Levato, H.; Gorbovskoy, E.; Tiurina, N.; Kuznetsov, A.; Vladimirov, V.; Kornilov, V.; Chazov, V.; Pogrosheva, T.; Potter, S.; Senik, V.; Dormidontov, D.; Parkhomenko, A.; Shurpakov, S.

    2016-10-01

    MASTER-Kislovodsk auto-detection system (Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L) discovered OT source at (RA, Dec) = 11h 05m 33.80s +19d 41m 18.7s on 2016-10-23.06338 UT. The OT unfiltered magnitude is 16.7m (mlim=18.8m).

  1. MASTER-SAAO: bright PSN in PGC343067

    NASA Astrophysics Data System (ADS)

    Shumkov, V.; Lipunov, V.; Buckley, D.; Gorbovskoy, E.; Tiurina, N.; Balanutsa, P.; Kuznetsov, A.; Kornilov, V.; Gress, O.; Ivanov, K.; Vladimirov, V.; Kuvshinov, D.; Potter, S.

    2016-10-01

    MASTER-SAAO auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 03h 33m 33.26s -62d 33m 14.7s on 2016-10-19.96568 UT. The OT unfiltered magnitude is (mlim=18.8).

  2. MASTER-IAC, MASTER-SAAO: optical transients discoveries

    NASA Astrophysics Data System (ADS)

    Shumkov, V.; Balanutsa, P.; Pogrosheva, T.; Rabinovich, J.; Lipunov, V.; Lopez, R. Rebolo; Ricart, M. Serra; Gorbovskoy, E.; Tiurina, N.; Balanutsa, P.; Kuznetsov, A.; Kornilov, V.; Gress, O.; Ivanov, K.; Vlasenko, D.; Shurpakov, S.; Kuvshinov, D.

    2016-09-01

    MASTER-IAC auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 349171 ) discovered OT source at (RA, Dec) = 23h 30m 43.39s -02d 04m 14.8s on 2016-09-08.984185 UT. The OT unfiltered magnitude is 16.8m (the limit is 20.1m).

  3. MASTER Net: optical transients

    NASA Astrophysics Data System (ADS)

    Gress, O.; Shumkov, V.; Pogrosheva, T.; Shurpakov, S.; Lipunov, V.; Buckley, D.; Lopez, R. Rebolo; Ricart, M. Serra; Podesta, R.; Levato, H. O.; Gorbovskoy, E.; Tiurina, N.; Balanutsa, P.; Kuznetsov, A.; Chazov, V.; Kornilov, V.; Ivanov, K.; Vladimirov, V.; Potter, S.; Lopez, C.; Podesta, F.; Saffe, C.

    2016-10-01

    MASTER-SAAO auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 14h 38m 49.60s -44d 37m 24.5s on 2016-10-01.73438 UT with unfiltered m_OT=16.4m (mlim=19.7m).

  4. MASTER discovery: PSN in PGC877124 and high amplitude dwarf nova

    NASA Astrophysics Data System (ADS)

    Pogrosheva, T.; Gress, O.; Shumkov, V.; Lipunov, V.; Buckley, D.; Rebolo Lopez, R.; Serra Ricart, M.; Podesta, R.; Levato, H.; Tiurina, N.; Gorbovskoy, E.; Balanutsa, P.; Kuznetsov, A.; Kornilov, V.; Vladimirov, V.; Kuvshinov, D.; Potter, S.; Lodieu, N.; Saffe, C.; Lopez, C.; Podesta, F.; Rabinovich, J.; Ivanov, K.; Budnev, N.

    2016-09-01

    MASTER-SAAO auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 01h 34m 15.01s -17d 48m 43.9s on 2016-09-24.00260 UT. The OT unfiltered magnitude is 17.5m (mlim=19.8m).

  5. PSN in PGC938372 discovered by MASTER

    NASA Astrophysics Data System (ADS)

    Shumkov, V.; Lipunov, V.; Buckley, D.; Gorbovskoy, E.; Tiurina, N.; Balanutsa, P.; Kuznetsov, A.; Kornilov, V.; Kuvshinov, D.; Vlasenko, D.; Gress, O.; Ivanov, K.; Porosheva, T.; Podesta, R. C.; Levato, O. H.; Lopez, C.; Podestan, F.; Saffe, C.; Potter, S.

    2016-09-01

    MASTER-IAC auto-detection system( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 05h 07m 27.73s -13d 24m 16.1s on 2016-09-06.23199 UT. The OT unfiltered magnitude is 17.2m (limit 19.4m).

  6. MASTER-OAFA discovery: 9mag amplitude outburst in SMC

    NASA Astrophysics Data System (ADS)

    Shumkov, V.; Lipunov, V.; Podesta, R.; Levato, H.; Tiurina, N.; Gorbovskoy, E.; Balanutsa, P.; Kornilov, V.; Chazov, V.; Kuznetsov, A.; Vlasenko, D.; Vladimirov, V.; Gress, O.; Ivanov, K.; Budnev, N.; Lopez, C.; Podesta, F.; Saffe, C.

    2016-10-01

    MASTER-OAFA auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L )discovered OT source at (RA, Dec) = 01h 06m 03.18s -74d 47m 15.8s on 2016-10-14.19341 UT. The OT magnitude in unfilter is (limit 18.3m).

  7. MASTER-OAFA discovery: 2 dwarf nova outbursts

    NASA Astrophysics Data System (ADS)

    Shumkov, V.; Pogrosheva, T.; Lipunov, V.; Podesta, R.; Levato, H.; Tiurina, N.; Gorbovskoy, E.; Balanutsa, P.; Kornilov, V.; Chazov, V.; Kuznetsov, A.; Vlasenko, D.; Vladimirov, V.; Gress, O.; Ivanov, K.; Lopez, C.; Podesta, F.; Saffe, C.

    2016-10-01

    MASTER-OAFA (located in Argentina) auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 20h 36m 45.27s -30d 15m 50.1s on 2016-10-14.10257 UT. The OT magnitude in unfiltered is (limit 18.4m).

  8. MASTER: bright PSN discovered in Argentina

    NASA Astrophysics Data System (ADS)

    Shumkov, V.; Lipunov, V.; Podesta, R.; Levato, H.; Buckley, D.; Gorbovskoy, E.; Tiurina, N.; Balanutsa, P.; Kuznetsov, A.; Vladimirov, V.; Gress, O.; Ivanov, K.; Chazov, V.; Lopez, C.; Podesta, F.; Saffe, C.; Pogrosheva, T.; Shurpakov, S.

    2016-10-01

    MASTER-OAFA (located in Argentina) auto-detection system ( Lipunov et al., 2010, Advances in Astronomy, vol. 2010, 30L ) discovered OT source at (RA, Dec) = 22h 01m 01.36s -40d 15m 26.7s on 2016-10-31.08091 UT. The OT unfiltered magnitude is 16.9m (mlim=19.9m).

  9. Molecular evolution of the neurohypophysial hormone precursors in mammals: Comparative genomics reveals novel mammalian oxytocin and vasopressin analogues.

    PubMed

    Wallis, Michael

    2012-11-01

    Among vertebrates the neurohypophysial hormones show considerable variation. However, in eutherian mammals they have been considered rather conserved, with arginine vasopressin (AVP) and oxytocin (OT) in all species except pig and some relatives, where lysine vasopressin replaces AVP. The availability of genomic data for a wide range of mammals makes it possible to assess whether these peptides and their precursors may be more variable in Eutheria than previously suspected. A survey of these data confirms that AVP and OT occur in most eutherians, but with exceptions. In a New-World monkey (marmoset, Callithrix jacchus) and in tree shrew (Tupaia belangeri), Pro(8)OT replaces OT, confirming a recent report for these species. In armadillo (Dasypus novemcinctus) Leu(3)OT replaces OT, while in tenrec (Echinops telfairi) Thr(4)AVP replaces AVP. In these two species there is also evidence for additional genes/pseudogenes, encoding much-modified forms of AVP, but in most other eutherian species there is no evidence for additional neurohypophysial hormone genes. Evolutionary analysis shows that sequences of eutherian neurohypophysial hormone precursors are generally strongly conserved, particularly those regions encoding active peptide and neurophysin. The close association between OT and VP genes has led to frequent gene conversion of sequences encoding neurophysins. A monotreme, platypus (Ornithorhynchus anatinus) has genes for OT and AVP, organized tail-to-tail as in eutherians, but in marsupials 3-4 genes are present for neurohypophysial hormones, organized tail-to-head as in lower vertebrates. PMID:22995712

  10. Using probabilistic modeling to evaluate human exposure to organotin in drinking water transported by polyvinyl chloride pipe.

    PubMed

    Fristachi, Anthony; Xu, Ying; Rice, Glenn; Impellitteri, Christopher A; Carlson-Lynch, Heather; Little, John C

    2009-11-01

    The leaching of organotin (OT) heat stabilizers from polyvinyl chloride (PVC) pipes used in residential drinking water systems may affect the quality of drinking water. These OTs, principally mono- and di-substituted species of butyltins and methyltins, are a potential health concern because they belong to a broad class of compounds that may be immune, nervous, and reproductive system toxicants. In this article, we develop probability distributions of U.S. population exposures to mixtures of OTs encountered in drinking water transported by PVC pipes. We employed a family of mathematical models to estimate OT leaching rates from PVC pipe as a function of both surface area and time. We then integrated the distribution of estimated leaching rates into an exposure model that estimated the probability distribution of OT concentrations in tap waters and the resulting potential human OT exposures via tap water consumption. Our study results suggest that human OT exposures through tap water consumption are likely to be considerably lower than the World Health Organization (WHO) "safe" long-term concentration in drinking water (150 microg/L) for dibutyltin (DBT)--the most toxic of the OT considered in this article. The 90th percentile average daily dose (ADD) estimate of 0.034 +/- 2.92 x 10(-4)microg/kg day is approximately 120 times lower than the WHO-based ADD for DBT (4.2 microg/kg day).

  11. Prenatal stress puzzle, the oxytocin piece: Prenatal stress alters the behaviour and autonomic regulation in piglets, insights from oxytocin

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Developmental changes in response to prenatal stressors (PNS) may represent an adaptive strategy to enhance survival traits in the offspring. Yet, PNS could be maladaptive for captive animals, causing anxiety and abnormal social development. Oxytocin (OT) reduces anxiety, whereas OT deficiencies are...

  12. Prenatally stressed piglets 'shut down' in response to separation, oxytocin modulates some effects

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Prenatal stress (PNS) effects may enhance offspring’s survival traits. Yet, PNS could be maladaptive for captive animals, causing anxiety and abnormal social development. Oxytocin (OT) reduces anxiety while OT deficiency results in social behavior alteration. We hypothesized that PNS piglets would b...

  13. 76 FR 39474 - Monthly Median Cost of Funds Reporting, and Publication of Cost of Funds Indices

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-06

    ... resulting from monetary policy actions. The OTS agrees. The OTS also finds that the MTA is less of a lagging... public comment (76 FR 7089) \\3\\ on its notice of intent to discontinue data collection and publication of... current manner using existing processes. \\3\\ Link to 76 FR 7089:...

  14. Clinical interpretation of opioid tolerance versus opioid-induced hyperalgesia.

    PubMed

    Chen, Lucy; Sein, Michael; Vo, Trang; Amhmed, Shihab; Zhang, Yi; Hilaire, Kristin St; Houghton, Mary; Mao, Jianren

    2014-01-01

    Opioid analgesics are commonly used to manage moderate to severe pain. However, the long-term use of opioids could lead to opioid tolerance (OT) and opioid-induced hyperalgesia (OIH). Distinguishing OIH from OT would impact the practice of opioid therapy because opioid dose adjustment may differentially influence OT and OIH. Currently, there are no standard criteria of OT versus OIH causing considerable ambiguity in clinical interpretation and management of these conditions. The authors designed a practitioner-based survey consisting of 20 targeted questions. Answering these questions would require responders' actual clinical experiences with opioid therapy. The survey was conducted between 2011 and 2012 through direct mails or e-mails to 1,408 physicians who are currently practicing in the United States. The authors find that certain clinical characteristics (eg, increased pain despite opioid dose escalation) are often used by practitioners to make differential diagnosis of OT and OIH despite some overlap in their clinical presentation. A key difference in clinical outcome is that OT and OIH could be improved and exacerbated by opioid dose escalation, respectively. Our survey results revealed a significant knowledge gap in some responders regarding differential diagnosis and management of OT and OIH. The results also identified several issues, such as opioid dose adjustment and clinical comorbidities related to OT and OIH, which require future patient-based studies.

  15. Oxytocin evokes a pulsatile PGE2 release from ileum mucosa and is required for repair of intestinal epithelium after injury.

    PubMed

    Chen, Dawei; Zhao, Junhan; Wang, Haoyi; An, Ning; Zhou, Yuping; Fan, Jiahui; Luo, Junwen; Su, Wenlong; Liu, Chuanyong; Li, Jingxin

    2015-07-10

    We measured the short-circuit current (Isc) in rat ileum mucosa to identify the effect of oxytocin (OT) on mucosal secretion in small intestine. We identified a COX-2-derived pulsatile PGE2 release triggered by OT in rat ileum mucosa. OT receptors (OTR) are expressed in intestine crypt epithelial cells. Notably, OT evoked a dynamic change of [Ca(2+)]i in ileum crypts, which was responsible for this pulsatile release of PGE2. OT ameliorated 5-FU-, radiation- or DSS- induced injury in vivo, including the improvement of weight loss, reduced villus height and impaired survival of crypt transit-amplifying cells as well as crypt. Moreover, these protective effects of OT against intestinal injury were eliminated by coadministration of a selective inhibitor of PGE2, AH6809. Our findings strongly suggest that OT, a novel and important regulator of intestine mucosa barrier, is required for repair of intestinal epithelium after injury. Considering that OT is an FDA-approved drug, this work reveals a potential novel and safe way to combat or prevent chemo-radiotherapy induced intestine injury or to treat IBD.

  16. 12 CFR Appendix F to Part 225 - Interagency Guidelines Establishing Information Security Standards

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Office of the Comptroller of the Currency (OCC), and the Office of Thrift Supervision (OTS). 2 12 CFR part 30, app. B (OCC); 12 CFR part 208, app. D-2 and part 225, app. F (Board); 12 CFR part 364, app. B (FDIC); and 12 CFR part 570, app. B (OTS). The “Interagency Guidelines Establishing Information...

  17. Similar ventral occipito-temporal cortex activations in literate and illiterate adults during the Chinese character matching task: an fMRI study.

    PubMed

    Qi, Geqi; Li, Xiujun; Yan, Tianyi; Wang, Bin; Yang, Jiajia; Wu, Jinglong; Guo, Qiyong

    2014-04-30

    Visual word expertise is typically associated with enhanced ventral occipito-temporal (vOT) cortex activation in response to written words. Previous study utilized a passive viewing task and found that vOT response to written words was significantly stronger in literate compared to the illiterate subjects. However, recent neuroimaging findings have suggested that vOT response properties are highly dependent upon the task demand. Thus, it is unknown whether literate adults would show stronger vOT response to written words compared to illiterate adults during other cognitive tasks, such as perceptual matching. We addressed this issue by comparing vOT activations between literate and illiterate adults during a Chinese character and simple figure matching task. Unlike passive viewing, a perceptual matching task requires active shape comparison, therefore minimizing automatic word processing bias. We found that although the literate group performed better at Chinese character matching task, the two subject groups showed similar strong vOT responses during this task. Overall, the findings indicate that the vOT response to written words is not affected by expertise during a perceptual matching task, suggesting that the association between visual word expertise and vOT response may depend on the task demand. PMID:24582905

  18. 12 CFR Appendix B to Part 364 - Interagency Guidelines Establishing Information Security Standards

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... this monitoring, a bank should review audits, summaries of test results, or other equivalent... (OTS). 2 12 CFR part 30, app. B (OCC); 12 CFR part 208, app. D-2 and part 225, app. F (Board); 12 CFR part 364, app. B (FDIC); and 12 CFR part 570, app. B (OTS). The “Interagency Guidelines...

  19. 12 CFR Appendix B to Part 570 - Interagency Guidelines Establishing Information Security Standards

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... by paragraph D.2. As part of this monitoring, you should review audits, summaries of test results, or... Office of Thrift Supervision (OTS). 2 12 CFR part 30, app. B (OCC); 12 CFR part 208, app. D-2 and part 225, app. F (Board); 12 CFR part 364, app. B (FDIC); and 12 CFR part 570, app. B (OTS)....

  20. 12 CFR Appendix B to Part 30 - Interagency Guidelines Establishing Information Security Standards

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... monitoring, a bank should review audits, summaries of test results, or other equivalent evaluations of its... Office of Thrift Supervision (OTS). 2 12 CFR part 30, app. B (OCC); 12 CFR part 208, app. D-2 and part 225, app. F (Board); 12 CFR part 364, app. B (FDIC); and 12 CFR part 570, app. B (OTS)....

  1. 12 CFR Appendix D-2 to Part 208 - Interagency Guidelines Establishing Information Security Standards

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... their obligations as required by paragraph D.2. As part of this monitoring, a bank should review audits... (OTS). 2 12 CFR part 30, app. B (OCC); 12 CFR part 208, app. D-2 and part 225, app. F (Board); 12 CFR part 364, app. B (FDIC); and 12 CFR part 570, app. B (OTS). The “Interagency Guidelines...

  2. The oxytocin system in drug discovery for autism: Animal models and novel therapeutic strategies

    PubMed Central

    Modi, Meera E.; Young, Larry J.

    2012-01-01

    Animal models and behavioral paradigms are critical for elucidating the neural mechanism involved in complex behaviors, including social cognition. Both genotype and phenotype based models have implicated the neuropeptide oxytocin (OT) in the regulation of social behavior. Based on the findings in animal models, alteration of the OT system has been hypothesized to play a role in the social deficits associated with autism and other neuropsychiatric disorders. While the evidence linking the peptide to the etiology of the disorder is not yet conclusive, evidence from multiple animal models suggest modulation of the OT system may be a viable strategy for the pharmacological treatment of social deficits. In this review, we will discuss how animal models have been utilized to understand the role of OT in social cognition and how those findings can be applied to the conceptualization and treatment of the social impairments in ASD. Animal models with genetic alterations of the OT system, like the OT, OT receptor and CD38 knock-out mice, and those with phenotypic variation in social behavior, like BTBR inbred mice and prairie voles, coupled with behavioral paradigms with face and construct validity may prove to have predictive validity for identifying the most efficacious methods of stimulating the OT system to enhance social cognition in humans. The widespread use of strong animal models of social cognition has the potential yield pharmacological, interventions for the treatment social impairments psychiatric disorders. This article is part of a Special Issue entitled Oxytocin, Vasopressin, and Social Behavior. PMID:22206823

  3. 32 CFR 3.9 - Follow-on production contracts.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... to the awardee of the OT prototype agreement for a specific number of units at specific target prices... proposed quantity and target prices for the follow-on production units as part of that competition. (3... the project. (4) Specify the production quantity and target prices in the OT prototype agreement...

  4. 32 CFR 3.9 - Follow-on production contracts.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... to the awardee of the OT prototype agreement for a specific number of units at specific target prices... proposed quantity and target prices for the follow-on production units as part of that competition. (3... the project. (4) Specify the production quantity and target prices in the OT prototype agreement...

  5. 12 CFR Appendix B to Part 563e - CRA Notice

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Appendix B to Part 563e—CRA Notice (a) Notice for main offices and, if an interstate savings association... each quarter, the OTS publishes a nationwide list of the savings associations that are scheduled for... address of official at savings association) and OTS (address). Your letter, together with any response...

  6. 12 CFR Appendix B to Part 563e - CRA Notice

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... Appendix B to Part 563e—CRA Notice (a) Notice for main offices and, if an interstate savings association... each quarter, the OTS publishes a nationwide list of the savings associations that are scheduled for... address of official at savings association) and OTS (address). Your letter, together with any response...

  7. 12 CFR Appendix B to Part 563e - CRA Notice

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... Appendix B to Part 563e—CRA Notice (a) Notice for main offices and, if an interstate savings association... each quarter, the OTS publishes a nationwide list of the savings associations that are scheduled for... address of official at savings association) and OTS (address). Your letter, together with any response...

  8. 12 CFR Appendix B to Part 563e - CRA Notice

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... Appendix B to Part 563e—CRA Notice (a) Notice for main offices and, if an interstate savings association... each quarter, the OTS publishes a nationwide list of the savings associations that are scheduled for... address of official at savings association) and OTS (address). Your letter, together with any response...

  9. 75 FR 22681 - Supplemental Guidance on Overdraft Protection Programs

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-29

    ... goals, including building long term customer relationships, cross selling additional products, and... Guidance on Overdraft Protection Programs (Overdraft Guidance) OTS issued February 18, 2005 (70 FR 8428). OTS issued the Overdraft Guidance after notice and comment. See 69 FR 31858 (June 7, 2004)....

  10. The Educational Interaction between Physical Therapy and Occupational Therapy Students.

    ERIC Educational Resources Information Center

    Cleary, Kimberly K.; Howell, Dana M.

    2003-01-01

    A survey of occupational therapy (OT) and physical therapy (PT) program directors (n=123) to identify the prevalence of shared learning found that two-thirds shared some coursework; most OT and PT students do not have opportunities to practice interdisciplinary teamwork; and some perceived benefits of shared learning also posed barriers. (Contains…

  11. Facile Peeling Method as a Post-Remedy Strategy for Producing an Ultrasmooth Self-Assembled Monolayer for High-Performance Organic Transistors.

    PubMed

    Chen, Xiaosong; Xu, Zeyang; Wu, Kunjie; Zhang, Suna; Li, Hongwei; Meng, Yancheng; Wang, Zhongwu; Li, Liqiang; Ma, Xueming

    2016-09-20

    The modification of dielectric surface with a self-assembled monolayer (SAM) such as octadecyltrichlorosilane (OTS) is a widely used method to tune the electrical property of diverse electronic devices based on organic semiconductors, graphene, transition metal dichalcogenides (TMDs), and so forth. The surface roughness of self-assembled OTS monolayer is a key factor in determining its effect on device performance, but the preparation of an ultrasmooth OTS monolayer is a technologically challenging task. In this work, an ultrasmooth OTS monolayer is prepared via a facile peeling method, which may serve as a postremedy strategy to remove the protuberant aggregates. Such a method has not been reported before. With organic semiconductors as a testing model, ultrasmooth OTS may significantly improve the charge mobility of organic field-effect transistors (OFETs). P-type dinaphtho[2,3-b:2',3'-f]thieno[3,2-b]thiophene (DNTT) OFET with an ultrasmooth OTS monolayer yields good reproducibility and unprecendented maximum mobility of 8.16 cm(2) V(-1) s(-1), which is remarkably superior to that of the OFET with a pristine OTS monolayer. This work develops a simple method to resolve the common and significant problem of the quality of OTS modification, which would be highly promising for electronic applications as well as other fields such as surface and interface engineering.

  12. Inclusion of Medical-Legal Issues in Entry-Level Occupational and Physical Therapy Curricula.

    ERIC Educational Resources Information Center

    Ekelman, Beth A.; Goodman, Glenn; Dal Bello-Haas, Vanina

    2000-01-01

    Directors of 47 occupational therapy (OT) and 65 physical therapy (PT) accredited entry-level programs responded to a survey by indicating their support for inclusion of medical-legal issues in the curriculum. Only 40% of OT and 52% of PT directors believed lawyers should deliver instruction; most felt their faculty were qualified to teach these…

  13. Collaborative Fieldwork Education with Student Occupational Therapists and Student Occupational Therapist Assistants.

    ERIC Educational Resources Information Center

    Jung, Bonny; Sainsury, Sandy; Grum, Rosa Maria; Wilkins, Seanne; Tryssenaar, Joyce

    2002-01-01

    Eight occupational therapy (OT) students and eight occupational therapy assistant (OTA) students worked together in field experiences. Content analysis of their journals revealed that they learned about each other's professional role and understood the relevance to future client care. Some resistance arose in both groups because OT students were…

  14. Multisource Feedback Systems for Quality Improvement in the Health Professions: Assessing Occupational Therapists in Practice

    ERIC Educational Resources Information Center

    Violato, Claudio; Worsfold, Leanne; Polgar, Jan Miller

    2009-01-01

    Introduction: The objective was to develop and psychometrically evaluate (feasibility, reliability, validity) a questionnaire-based multisource feedback (MSF) system for quality improvement (QI) for occupational therapists (OTs). Methods: Surveys were developed for assessment of OTs by clients, co-workers, and themselves, respectively, using…

  15. The Role of Occupational Therapists and Physical Therapists in Elementary School System Early Intervening Services and Response to Intervention: A Case Report

    ERIC Educational Resources Information Center

    Reeder, Deborah L.; Arnold, Sandra H.; Jeffries, Lynn M.; McEwen, Irene R.

    2011-01-01

    The Individuals with Disabilities Improvement Act and No Child Left Behind Act broadened the roles of occupational therapists (OTs) and physical therapists (PTs) to include therapist participation in early intervening services including response to intervention (RTI). This case report describes one school district's inclusion of OT and PT in the…

  16. The Effect of Online Translators on L2 Writing in French

    ERIC Educational Resources Information Center

    O'Neill, Errol Marinus

    2012-01-01

    Online translation (OT) sites such as Free Translation and Babel Fish are freely available to the general public and purport to convert inputted text, from single words to entire paragraphs, instantly from one language to another. Discussion in the literature about OT for second-language (L2) acquisition has generally focused either on the…

  17. 12 CFR 570.1 - Authority, purpose, scope and preservation of existing authority.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... part are issued by the OTS under section 39 (section 39) of the Federal Deposit Insurance Act (FDI Act.... L. 106-102, 113 Stat. 1338 (1999)). (b) Purpose. Section 39 of the FDI Act requires the OTS to... guideline under section 39 (a) or (b). An enforceable order under section 8 of the FDI Act may be issued...

  18. 12 CFR 565.7 - Directives to take prompt corrective action.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... OTS's discretion to require or impose under section 38 of the FDI Act, including sections 38(e)(5), (f... directive. If the OTS finds it necessary in order to carry out the purposes of section 38 of the FDI Act... require or impose under section 38 of the FDI Act, including section 38(e)(5), (f)(2), (f)(3), or...

  19. 12 CFR 570.5 - Enforcement of orders.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... association fails to comply with an order issued under section 39 of the FDI Act, the OTS may seek enforcement of the order in the appropriate United States district court pursuant to section 8(i)(1) of the FDI Act. (b) Administrative remedies. Pursuant to section 8(i)(2)(A) of the FDI Act, the OTS may assess...

  20. Oxytocin mediates social neuroprotection after cerebral ischemia

    PubMed Central

    Karelina, Kate; Stuller, Kathleen A.; Jarrett, Brant; Zhang, Ning; Wells, Jackie; Norman, Greg J.; DeVries, A. Courtney

    2011-01-01

    Background and Purpose The reduced incidence, morbidity and mortality of stroke among humans with strong social support have been well-documented; however, the mechanisms underlying this socially mediated phenomenon remain unknown, but may involve oxytocin (OT), a hormone that modulates some aspects of social behavior in humans and other animals. Methods In the present study, adult male mice were socially isolated (housed individually) or socially paired (housed with an ovariectomized female); social pairing increased hypothalamic OT gene expression. To determine whether a causal relationship exists between increased OT and improved stroke outcome, mice were treated with exogenous OT or OT receptor antagonist (OTA) beginning one week prior to induction of experimental stroke via middle cerebral artery occlusion (MCAO). Results Relative to social isolation, social housing attenuated infarct size, neuroinflammation, and oxidative stress following experimental stroke; the neuroprotective effect of social housing was eliminated by OTA treatment. In contrast, administration of OT to socially isolated mice reproduced the neuroprotection conferred by social housing. We further report evidence for a direct suppressive action of OT on cultured microglia, which is a key instigator in the development of neuroinflammation after cerebral ischemia. Conclusions These findings support the hypothesis that OT mediates the neuroprotective effect of social interaction on stroke outcome. PMID:21960564

  1. Effects of Online Translation on Morphosyntactic and Lexical-Pragmatic Accuracy in Essay Writing in Spanish as a Foreign Language

    ERIC Educational Resources Information Center

    Fredholm, Kent

    2014-01-01

    The use of online translation (OT) is increasing as more pupils receive laptops from their schools. This study investigates OT use in two groups of Swedish pupils (ages 17-18) studying Spanish as an L3: one group (A) having free Internet access and the spelling and grammar checker of Microsoft Word, the other group (B) using printed dictionaries…

  2. Oxytocin modulates fMRI responses to facial expression in macaques.

    PubMed

    Liu, Ning; Hadj-Bouziane, Fadila; Jones, Katherine B; Turchi, Janita N; Averbeck, Bruno B; Ungerleider, Leslie G

    2015-06-16

    Increasing evidence has shown that oxytocin (OT), a mammalian hormone, modifies the way social stimuli are perceived and the way they affect behavior. Thus, OT may serve as a treatment for psychiatric disorders, many of which are characterized by dysfunctional social behavior. To explore the neural mechanisms mediating the effects of OT in macaque monkeys, we investigated whether OT would modulate functional magnetic resonance imaging (fMRI) responses in face-responsive regions (faces vs. blank screen) evoked by the perception of various facial expressions (neutral, fearful, aggressive, and appeasing). In the placebo condition, we found significantly increased activation for emotional (mainly fearful and appeasing) faces compared with neutral faces across the face-responsive regions. OT selectively, and differentially, altered fMRI responses to emotional expressions, significantly reducing responses to both fearful and aggressive faces in face-responsive regions while leaving responses to appeasing as well as neutral faces unchanged. We also found that OT administration selectively reduced functional coupling between the amygdala and areas in the occipital and inferior temporal cortex during the viewing of fearful and aggressive faces, but not during the viewing of neutral or appeasing faces. Taken together, our results indicate homologies between monkeys and humans in the neural circuits mediating the effects of OT. Thus, the monkey may be an ideal animal model to explore the development of OT-based pharmacological strategies for treating patients with dysfunctional social behavior. PMID:26015576

  3. Community-Based Occupational Therapy Services for Children: A Quebec Survey on Service Delivery

    ERIC Educational Resources Information Center

    Cotellesso, Annie; Mazer, Barbara; Majnemer, Annette

    2009-01-01

    Community-based occupational therapy (OT) services are intended to promote social integration and minimize disability. The objective of this study was to describe community-based OT services for children in the province of Quebec, Canada. Specific aims included (a) to determine the proportion of Centres Locaux de Services Communautaires (CLSCs)…

  4. 12 CFR 563b.660 - What must I include in my voluntary supervisory conversion application?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ..., or an IRS ruling indicating that the transaction qualifies as a tax-free reorganization. (3) An... description of your estimated conversion expenses. (3) Evidence supporting the value of any non-cash asset contributions. Appraisals must be acceptable to OTS and the non-cash asset must meet all other OTS...

  5. 12 CFR 543.1 - Corporate title.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ...-INCORPORATION, ORGANIZATION, AND CONVERSION § 543.1 Corporate title. (a) General. A Federal savings association...) Title change. Prior to changing its corporate title, an association must file with the OTS a written notice indicating the intended change. The OTS, shall provide to the association a timely...

  6. Intranasal oxytocin increases social grooming and food sharing in the common vampire bat Desmodus rotundus.

    PubMed

    Carter, Gerald G; Wilkinson, Gerald S

    2015-09-01

    Intranasal oxytocin (OT) delivery has been used to non-invasively manipulate mammalian cooperative behavior. Such manipulations can potentially provide insight into both shared and species-specific mechanisms underlying cooperation. Vampire bats are remarkable for their high rates of allogrooming and the presence of regurgitated food sharing among adults. We administered intranasal OT to highly familiar captive vampire bats of varying relatedness to test for an effect on allogrooming and food sharing. We found that intranasal OT did not have a detectable effect on food-sharing occurrence, but it did increase the size of regurgitated food donations when controlling for dyad and amount of allogrooming. Intranasal OT in females increased the amount of allogrooming per partner and across all partners per trial, but not the number of partners. We also found that the peak effect of OT treatments occurred 30-50min after administration, which is consistent with the reported latency for intranasal OT to affect relevant brain areas in rats and mice. Our results suggest that intranasal OT is a potential tool for influencing dyadic cooperative investments, but measuring prior social relationships may be necessary to interpret the results of hormonal manipulations of cooperative behavior and it may be difficult to alter partner choice in vampire bats using intranasal OT alone. PMID:26475061

  7. 12 CFR 567.11 - Reservation of authority.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... capital instrument constitutes or may constitute core or supplementary capital, and may permit one or more...) Notwithstanding § 567.6 of this part, OTS will look to the substance of a transaction and may find that the.... OTS will look to the substance of and risk associated with the transaction as well as other...

  8. 12 CFR Appendix A to Part 563e - Ratings

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... rating profile as follows. (b) Savings associations evaluated under the lending, investment, and service tests—(1) Lending performance rating. The OTS assigns each savings association's lending performance one of the five following ratings. (i) Outstanding. The OTS rates a savings association's...

  9. 12 CFR 575.9 - Charters and bylaws for mutual holding companies and their savings association subsidiaries.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... by the OTS, 12 CFR part 552, or by the appropriate state chartering authority, as the case may be... accordance with the purchase priorities set forth in 12 CFR part 563b, include in its charter the following... null and void and shall be returned to the OTS within five days. (4) Promptly after approval of...

  10. 32 CFR 3.6 - Limitations on cost-sharing.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... THAN CONTRACTS, GRANTS, OR COOPERATIVE AGREEMENTS FOR PROTOTYPE PROJECTS § 3.6 Limitations on cost... prototype project and cost-sharing is the reason for using OT authority, then the non-Federal amounts... the OT agreement becomes effective. Costs that were incurred for a prototype project by the...

  11. 32 CFR 3.6 - Limitations on cost-sharing.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... THAN CONTRACTS, GRANTS, OR COOPERATIVE AGREEMENTS FOR PROTOTYPE PROJECTS § 3.6 Limitations on cost... prototype project and cost-sharing is the reason for using OT authority, then the non-Federal amounts... the OT agreement becomes effective. Costs that were incurred for a prototype project by the...

  12. 32 CFR 3.6 - Limitations on cost-sharing.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... THAN CONTRACTS, GRANTS, OR COOPERATIVE AGREEMENTS FOR PROTOTYPE PROJECTS § 3.6 Limitations on cost... prototype project and cost-sharing is the reason for using OT authority, then the non-Federal amounts... the OT agreement becomes effective. Costs that were incurred for a prototype project by the...

  13. 32 CFR 3.6 - Limitations on cost-sharing.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... THAN CONTRACTS, GRANTS, OR COOPERATIVE AGREEMENTS FOR PROTOTYPE PROJECTS § 3.6 Limitations on cost... prototype project and cost-sharing is the reason for using OT authority, then the non-Federal amounts... the OT agreement becomes effective. Costs that were incurred for a prototype project by the...

  14. Working in the Methodological "Outfield": The Case of Bourdieu and Occupational Therapy

    ERIC Educational Resources Information Center

    Watson, Jo; Grenfell, Michael

    2016-01-01

    The article reports on a study of methodological innovation involving occupational therapy (OT) students in higher education (HE). It is based on an original project which examined the experiences and outcomes of non-traditional entrants to pre-registration OT education. A feature of the original project was the application of the epistemological…

  15. MASTER-SAAO: PSN in possibly AGN galaxy and 2 new dwarf nova outbursts

    NASA Astrophysics Data System (ADS)

    Shumkov, V.; Balanutsa, P.; Lipunov, V.; Buckley, D.; Gorbovskoy, E.; Tyurina, N.; Kuznetsov, A.; Kornilov, V.; Kuvshinov, D.; Vlasenko, D.; Potter, S.; Kniazev, A.; Budnev, N.; Gress, O.; Ivanov, K.

    2016-08-01

    MASTER-SAAO auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 00h 17m 06.60s -38d 22m 24.8s on 2016-08-09.01954 UT. The OT unfiltered magnitude is 18.6m (mlim=20.4m).

  16. MASTER: possible superluminous SN, QSO flare and new dwarf novae outbursts

    NASA Astrophysics Data System (ADS)

    Balanutsa, P.; Gress, O.; Popova, E.; Lipunov, V.; Buckley, D.; Lopez, R. Rebolo; Serra-Ricart, M.; Tlatov, A.; Gorbovskoy, E.; Tyurina, N.; Kuznetsov, A.; Kornilov, V.; Kuvshinov, D.; Vlasenko, D.; Shumkov, V.; Shurpakov, S.; Potter, S.; Kniazev, A.; Senik, V.; Dormidontov, D.; Parhomenko, A. V.; Ivanov, K.; Budnev, N.; Yurkov, V.; Sergienko, Yu.; Gabovich, A.

    2016-08-01

    MASTER-IAC auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L )discovered OT source at (RA, Dec) = 22h 59m 39.54s +02d 08m 59.6s on 2016-08-02.06248 UT. The OT unfiltered magnitude is 17.8m (lim=20.1m).

  17. MASTER: bright dwarf nova outburst

    NASA Astrophysics Data System (ADS)

    Shumkov, V.; Gress, O.; Lipunov, V.; Lopez, R. Rebolo; Serra-Ricart, M.; Gorbovskoy, E.; Tyurina, N.; Balanutsa, P.; Kuznetsov, A.; Kornilov, V.; Kuvshinov, D.; Popova, E.; Vlasenko, D.; Pogrosheva, T.

    2016-08-01

    MASTER-IAC auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 19h 52m 39.94s +51d 41m 56.4s on 2016-08-06.96659 UT. The OT unfiltered magnitude is 16.9m (limit 19.4m).

  18. MASTER: 2 optical transients

    NASA Astrophysics Data System (ADS)

    Vladimirov, V.; Shumkov, V.; Lipunov, V.; Buckley, D.; Lopez, R. Rebolo; Serra-Ricart, M.; Gorbovskoy, E.; Tiurina, N.; Balanutsa, P.; Kornilov, V.; Kuvshinov, D.; Kuznetsov, A.; Chazov, V.

    2016-08-01

    MASTER-SAAO auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 15h 25m 39.43s -70d 22m 45.1s on 2016-08-16.80259 UT. The OT unfiltered magnitude is 17.0m (mlim=17.9m).

  19. MASTER: bright PSN in NGC 0888

    NASA Astrophysics Data System (ADS)

    Shumkov, V.; Lipunov, V.; Buckley, D.; Podesta, R.; Levato, H.; Gorbovskoy, E.; Tyurina, N.; Balanutsa, P.; Kuznetsov, A.; Kornilov, V.; Kuvshinov, D.; Vladimirov, V.; Potter, S.; Kniazev, A.; Budnev, N.; Gress, O.; Ivanov, K.; Lopez, C.; Mallamaci, C.; Lopez, C.; Podesta, F.; Pogrosheva, T.

    2016-08-01

    MASTER-SAAO auto-detection system( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 02h 17m 29.29s -59d 51m 23.9s on 2016-08-23.12639 UT. The OT unfiltered magnitude is (mlim=19.1m).

  20. MASTER: PSN in Lick catalog's galaxy

    NASA Astrophysics Data System (ADS)

    Tiurina, N.; Lipunov, V.; Buckley, D.; Gorbovskoy, E.; Balanutsa, P.; Shumkov, V.; Kornilov, V.; Kuvshinov, D.; Pogrosheva, T.; Kuznetsov, A.; Vlasenko, D.; Potter, S.; Kniazev, A.; Budnev, N.; Gress, O.; Ivanov, K.

    2016-08-01

    MASTER-SAAO auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 04h 20m 32.78s -21d 52m 12.3s on 2016-08-16.03583 UT. The OT unfiltered magnitude is (limit 17.9m).