Sample records for kriis tnis kanger

  1. Mobility and Economic Feasibility of the Greenland Inland Traverse (GrIT)

    DTIC Science & Technology


    towing four GrIT08 bladder sleds at NEEM . ........................................... 29 Figure 15. John Deere 8530 tractor equipped with Soucy tracks...19 Table 2. LC130 parameters for flights from Kanger to NEEM and Summit. ...................................... 36 Table 3. Summary...initiated a 705-mile (1410-mile round trip) over-snow traverse, de- parting from Thule Air Base, to re-supply the international drilling camp at NEEM and

  2. Molecular evolution of troponin I and a role of its N-terminal extension in nematode locomotion

    PubMed Central

    Barnes, Dawn E.; Hwang, Hyundoo; Ono, Kanako; Lu, Hang; Ono, Shoichiro


    Summary The troponin complex, composed of troponin T (TnT), troponin I (TnI), and troponin C (TnC), is the major calcium-dependent regulator of muscle contraction, which is present widely in both vertebrates and invertebrates. Little is known about evolutionary aspects of troponin in the animal kingdom. Using a combination of data mining and functional analysis of TnI, we report evidence that an N-terminal extension of TnI is present in most of bilaterian animals as a functionally important domain. Troponin components have been reported in species in most of representative bilaterian phyla. Comparison of TnI sequences shows that the core domains are conserved in all examined TnIs, and that N- and C-terminal extensions are variable among isoforms and species. In particular, N-terminal extensions are present in all protostome TnIs and chordate cardiac TnIs but lost in a subset of chordate TnIs including vertebrate skeletal-muscle isoforms. Transgenic rescue experiments in C. elegans striated muscle show that the N-terminal extension of TnI (UNC-27) is required for coordinated worm locomotion but not in sarcomere assembly and single muscle-contractility kinetics. These results suggest that N-terminal extensions of TnIs are retained from a TnI ancestor as a functional domain. PMID:26849746

  3. Development of the EURITRACK tagged neutron inspection system

    NASA Astrophysics Data System (ADS)

    Perot, B.; Carasco, C.; Bernard, S.; Mariani, A.; Szabo, J.-L.; Mercier, E.; Sannie, G.; Viesti, G.; Nebbia, G.; Pesente, S.; Lunardon, M.; Moretto, S.; Fabris, D.; Zenoni, A.; Bonomi, G.; Donzella, A.; Fontana, A.; Boghen, G.; Valkovic, V.; Sudac, D.; Moszynski, M.; Batsch, T.; Gierlik, M.; Woski, D.; Klamra, W.; Isaksson, P.; Le Tourneur, P.; Lhuissier, M.; Colonna, A.; Tintori, C.; Peerani, P.; Sequeira, V.; Salvato, M.


    The EURopean Illicit TRAfficing Countermeasures Kit (EURITRACK) project is part of the 6th European Union Framework Program. It aims at developing a Tagged Neutron Inspection System (TNIS) to detect illicit materials, such as explosives and narcotics, in cargo containers. Fast neutron induced reactions produce specific gamma-rays used to determine the chemical composition of the inspected material. The associated particle technique is employed to precisely locate the interaction points of the neutrons. A new deuterium-tritium neutron generator has been developed, including a pixelized alpha particle detector. The TNIS also comprises high-efficiency fast neutron and gamma-ray detectors, a dedicated front-end electronics and an integrated software to entirely drive the system and automatically process the data. Most components have been integrated during last months at Institute Ruder Boskovic, in Zagreb, Croatia. An overview of the TNIS and of its preliminary performances is presented.

  4. The slow isoform of Xenopus troponin I is expressed in developing skeletal muscle but not in the heart.


    Warkman, Andrew S; Atkinson, Burr G


    In birds and mammals three isoforms of troponin I (TnI) exist; a slow (TnIs), a fast (TnIf) and a cardiac (TnIc). Although each of these isoforms is expressed in the adult forms of these organisms in a muscle fiber-type-specific manner, the gene encoding TnIs is also expressed within the developing heart of these vertebrates. Herein, our results demonstrate that the developing heart of Xenopus laevis, unlike its counterpart in birds and mammals, does not express the gene encoding the TnIs isoform and that the expression of this gene, as well as the one encoding the Xenopus TnIf isoform, is restricted to skeletal muscle.

  5. Criteria for Reconstitution of Forces.

    DTIC Science & Technology


    proposed. ,.. nceS’ F For NTIS CQ𔃽*! DTI~C Vi LI Just If .ut - . ion_ .. ".. D 1lI T . .. .. .. ... AVAt 1 " t’ ’. Dist SIRCURITY CLASSIFICATION OF TNIS...the CGSC sample had combat experience, an average of 14 months. Only 4 percent of the Advanced Course respondents, who were mostly captains , reported

  6. Nerve-responsive troponin I slow promoter does not respond to unloading

    NASA Technical Reports Server (NTRS)

    Criswell, D. S.; Hodgson, V. R.; Hardeman, E. C.; Booth, F. W.


    We examined the regulation of the troponin I slow (TnIs) promoter during skeletal muscle unloading-induced protein isoform transition, by using a transgenic mouse line harboring the -4,200 to +12 base pairs region of the human TnIs promoter. Eighteen female transgenic mice ( approximately 30 g body mass) were randomly divided into two groups: weight-bearing (WB) controls (n = 9) and hindlimb unloaded (HU; n = 9). The HU mice were tail suspended for 7 days. Body mass was unchanged in the WB group but was reduced (-6%; P < 0.05) after the HU treatment. Absolute soleus muscle mass (-25%) and soleus mass relative to body mass (-16%) were both lower (P < 0.05) in the HU group compared with the WB mice. Northern blot analyses indicate that 7 days of HU result in a 64% decrease (P < 0.05) in the abundance of endogenous TnIs mRNA (microg/mg muscle) in the mouse soleus. Furthermore, there is a trend for the abundance of the fast troponin I mRNA to be increased (+34%). Analysis of transgenic chloramphenicol acetyltransferase activity in the soleus muscle revealed no difference (P > 0.05) between WB and HU groups. We conclude that additional elements are necessary for the TnIs gene to respond to an unloading-induced, slow-to-fast isoform transition stimulus.

  7. Electron Resonance Decay into a Biological Function: Decrease in Viability of E. coli Transformed by Plasmid DNA Irradiated with 0.5-18 eV Electrons.


    Kouass Sahbani, S; Cloutier, P; Bass, A D; Hunting, D J; Sanche, L


    Transient negative ions (TNIs) are ubiquitous in electron-molecule scattering at low electron impact energies (0-20 eV) and are particularly effective in damaging large biomolecules. Because ionizing radiation generates mostly 0-20 eV electrons, TNIs are expected to play important roles in cell mutagenesis and death during radiotherapeutic cancer treatment, although this hypothesis has never been directly verified. Here, we measure the efficiency of transforming E. coli bacteria by inserting into the cells, pGEM-3ZfL(-) plasmid DNA that confers resistance to the antibiotic ampicillin. Before transformation, plasmids are irradiated with electrons of specific energies between 0.5 and 18 eV. The loss of transformation efficiency plotted as a function of irradiation energy reveals TNIs at 5.5 and 9.5 eV, corresponding to similar states observed in the yields of DNA double strand breaks. We show that TNIs are detectable in the electron-energy dependence of a biological process and can decrease cell viability.

  8. Logic Design of a Shared Disk System in a Multi-Micro Computer Environment.

    DTIC Science & Technology


    thesis was essential to the project. Chapter II addresses tne system arcnitecture. Detailed discussion is eiven of all major hardware components as...tne development of tne vicropoiis interface design. A eeneral understandin? of tnis structure is an essential prerequiste to an understanding of,bin_dec,dec_asc • subroutine prints out at tne console a bulation of user supplied input and tne calling program

  9. Scanning Cargo Containers with Tagged Neutrons

    SciTech Connect

    Viesti, G.; Botosso, C.; Fabris, D.; Lunardon, M.; Moretto, S.; Nebbia, G.; Pesente, S.; Zenoni, A.; Donzella, A.; Perot, B.; Carasco, C.; Bernard, S.; Mariani, A.; Szabo, J.-L.; Sannie, G.; Valkovic, V.; Sudac, D.; Nad, K.; Peerani, P.; Sequeira, V.


    A new Tagged Neutron Inspection System (TNIS) able to detect illicit materials such as explosives and narcotics in cargo containers has been developed within the EURopean Illicit TRAfficing Countermeasures Kit (EURITRACK) project. After the R and D phase, the inspection portal has been installed and commissioned at the Rijeka seaport in Croatia, where it has been operated in connection with the existing X-ray scanner for a first two-month demonstration campaign. Results obtained are presented and discussed in this paper.

  10. Scanning Cargo Containers with Tagged Neutrons

    NASA Astrophysics Data System (ADS)

    Viesti, G.; Botosso, C.; Fabris, D.; Lunardon, M.; Moretto, S.; Nebbia, G.; Pesente, S.; Zenoni, A.; Donzella, A.; Perot, B.; Carasco, C.; Bernard, S.; Mariani, A.; Szabo, J.-L.; Sannie, G.; Valkovic, V.; Sudac, D.; Nad, K.; Peerani, P.; Sequeira, V.; Salvato, M.; Moszynski, M.; Gierlik, M.; Klamra, W.; Le Tourneur, P.; Lhuissier, M.; Colonna, A.; Tintori, C.


    A new Tagged Neutron Inspection System (TNIS) able to detect illicit materials such as explosives and narcotics in cargo containers has been developed within the EURopean Illicit TRAfficing Countermeasures Kit (EURITRACK) project. After the R&D phase, the inspection portal has been installed and commissioned at the Rijeka seaport in Croatia, where it has been operated in connection with the existing X-ray scanner for a first two-month demonstration campaign. Results obtained are presented and discussed in this paper.

  11. The Effect of Remote Sensor Spatial Resolution in Monitoring U.S. Army Training Maneuver Sites

    DTIC Science & Technology


    at Pinon Canyon and the abbreviations used in tnis research. 68 BOGR /HIJA 10-20% COVER Blue grama (Bouteloua gracilis) and Galleta (Hilaria 1 amesii...approximately 10-20% ground cover. BOGR /AGSM/HIJA 20-30% Blue grama (Bouteloua COVER gracilis) , Western wheatgrass (Agropyron smithii) and Galleta...Hilaria Jamesii), approximately 20-30% ground cover. BOGR /SPAI/HIJA 30-40% Blue grama (Bouteloua COVER gracilis), Alkali sacaton (Sporobolus airoides

  12. Compilation of Reprints Number 63.

    DTIC Science & Technology


    uO*-54 603 ICURITY CLASSIFICATION O0P TNIS PAGE (ft" 001- 010.01 Uc I ass i ,.d sa masi CLAsSIFICATzoui or mi$ PlCTiwnlho base rd depends on...1982). Elastic Scattering (ES). The categorizing of these interactions his facilitated the mathematical treatment as well as discussions of Dynamics...and medical sciences. Interstate Printers and Pub- lishers. Danville. III. 171 pp. University of Washington, Department of Oceanography. 1980. FRONTS

  13. Liquid Surface Levitation Holography. Part 1. Theoretical Analysis.

    DTIC Science & Technology


    Continued) ’ DD J AN 73 1473 EDITION OF INOV 65 IS OSSOLETE 0102-014-6601 SECURI1TY CLASSIFICATION OF TNIS PAGE (When, DIte Writoeed) Fig. 1. The acoustic energy from the object transducer passes into the object to be imaged and is diffracted, internally reflected, and attenuated...frequencies for a typical plastic membrane separating water and Freon E-5 (imaging fluid). Hence the energy in the internally reflected waves will

  14. A Microcomputer System for Target Information in the Fire Support Coordination Center: A Data Base Approach.

    DTIC Science & Technology


    space and lie speed of access to secondary storaee and. the requirement for an e..eftive interactive system for a non-sopbisticated user. The desian of...operator woriine in the SACC operational spaces uses tne QUEST data base query language to access targets and target information from the data base. Requests...display unit in the SACC. The report printouts are available from a printer located in the main computer spaces . During tnis period, the TIO is

  15. Low-Level Convergence and the Prediction of Convective Precipitation in South Florida.

    DTIC Science & Technology


    Af" -Ao97 55 ILLINOIS STATE WATER SURVEY URBANA F/6 4/2 LOWLEVEL CONVERGENCE AND THE PREDICTION OF CONVECTIVE PRECIPIT-ETC(U) FEB 81 A I WATSON. R L...not be construed as an official Department of the Army position, policy, or decision, unless so designated by other documentation. IS. KEY WORDS ...varying meteorological cond’tions and ti: == UNCLASSIFIED of day. SECURITY CLASSIFICATIOR OF TNIS PAGE (Whven Data Etered) State Water Survey

  16. Semidiurnal Hough Mode Extensions in the Thermosphere and Their Application

    DTIC Science & Technology


    OF TNIS PAGE (11t~n Veto Rnforod) ..- q4ITY CLASSIFICATION OF THIS PAGE(Whan Dae EFneld) 20. Abstract (Continued) solar cycle. This note gives the...will vary with solar cycle insofar as ion drag (proportional to electron density) and neutral temperature vary with the solar cycle. To present the...Reidel, Dordrecht, Holland, 200 pp. 2. Hong, S., and R. S. Lindzen, 1976, Solar Semidiurnal Tide in the Thermosphere. J. Atmos. Sci., a, in press. 3

  17. Distributed Hypothesis Formation in Distributed Sensor Networks

    DTIC Science & Technology


    the processing architecture at each node in the DSN. Each node contains the Generalized Tracker/Classifier for processing of (Continued) - DD 1473...TIM Olr TNiS PAG Iwhn D-le Ine 4e.) local sensor data, an information fusion module to integrate processed information from various nodes, and an...OF EACH PROCESSING NODE .. ............ 10 2.2.1 Local Processing of the Sensor Data. ......... 12 2.2.2 Information Fusion. .. ................ 12

  18. Transcriptional control of muscle plasticity: differential regulation of troponin I genes by electrical activity.


    Calvo, S; Stauffer, J; Nakayama, M; Buonanno, A


    Plasticity of the skeletal muscle phenotype can result from the selective repression and activation of gene expression in response to innervation patterns. Motoneurons, eliciting different patterns of depolarization, regulate the contractile properties of the myofibers they innervate by selectively activating expression of genes encoding fiber-type-specific (fast vs. slow) contractile proteins. We have analyzed the regulation of the troponin I slow (TnIs) and fast (TnIf) genes as a model to study the molecular mechanisms regulating fiber-type plasticity. We found that expression of the two TnI isoforms is downregulated by denervation. Moreover, TnI expression is upregulated by specific patterns of electrical activity [10 Hz vs. 100 Hz] used to depolarize muscle. We previously isolated the rat TnIs gene and demonstrated that regulatory sequences reside in its upstream region and second intron [Banerjee-Basu S, Buonanno A (1993), Mol Cell Biol 12:5024-5032]. Using transgenic mice, we show that the upstream region of the TnIs gene extending from -949 to +50 is sufficient to confer transcription specifically in slowtwitch muscles. Serial deletions of the TnIs upstream and intronic regions were generated in a CAT reporter vector to delineate transcriptional regulatory elements in transiently transfected Sol8 myotubes. Sequences necessary to confer the highest levels of TnIs transcription mapped to the upstream region between -0.95 and -0.72 kb, and to a 56 bp sequence located in the second intron. Comparison of the at sequence between -0.95 and -0.72 to the human TnIs gene identified a highly homologous region of 128 bp that we named the TnI SURE (slow upstream regulatory element). Alignment of these two SURE sequences with the quail TnI fast intronic regulatory element identified common motifs, namely, two A/T-rich sequences (A/T1 and A/T2) with homology to homeotic protein and MEF2 binding sites, a CACC box, an E box, and a novel motif (GCAGGCA) that we denoted the

  19. Tau-positive nuclear indentations in P301S tauopathy mice.


    Fernández-Nogales, Marta; Santos-Galindo, María; Merchán-Rubira, Jesús; Hoozemans, Jeroen J M; Rábano, Alberto; Ferrer, Isidro; Avila, Jesús; Hernández, Félix; Lucas, José J


    Increased incidence of neuronal nuclear indentations is a well-known feature of the striatum of Huntington's disease (HD) brains and, in Alzheimer's disease (AD), neuronal nuclear indentations have recently been reported to correlate with neurotoxicity caused by improper cytoskeletal/nucleoskeletal coupling. Initial detection of rod-shaped tau immunostaining in nuclei of cortical and striatal neurons of HD brains and in hippocampal neurons of early Braak stage AD led us to coin the term "tau nuclear rods (TNRs)." Although TNRs traverse nuclear space, they in fact occupy narrow cytoplasmic extensions that fill indentations of the nuclear envelope and we will here refer to this histological hallmark as Tau-immunopositive nuclear indentations (TNIs). We reasoned that TNI formation is likely secondary to tau alterations as TNI detection in HD correlates with an increase in total tau, particularly of the isoforms with four tubulin binding repeats (4R-tau). Here we analyze transgenic mice that overexpress human 4R-tau with a frontotemporal lobar degeneration-tau point mutation (P301S mice) to explore whether tau alteration is sufficient for TNI formation. Immunohistochemistry with various tau antibodies, immunoelectron microscopy and double tau-immunofluorescence/DAPI-nuclear counterstaining confirmed that excess 4R-tau in P301S mice is sufficient for the detection of abundant TNIs that fill nuclear indentations. Interestingly, this does not correlate with an increase in the number of nuclear indentations, thus suggesting that excess total tau or an isoform imbalance in favor of 4R-tau facilitates tau detection inside preexisting nuclear indentations but does not induce formation of the latter. In summary, here we demonstrate that tau alteration is sufficient for TNI detection and our results suggest that the neuropathological finding of TNIs becomes a possible indicator of increased total tau and/or increased 4R/3R-tau ratio in the affected neurons apart from being an

  20. Effects of Condensate Inundation and Vapor Velocity on Heat Transfer in a Condenser Tube Bundle.

    DTIC Science & Technology


    meter and the tank’s recirculation valve, the flow rate to the porous tube could be controlled with reasonable accuracy. The system was used to supply... flow rate of water to the desuper- heater spray nozzles via the rotameter. 150 5. Porous Tube Water Supply System a. Once steady state conditions have... system operated at test condenser pressures of approximately 2 and 15 DD ~T 1413 EmnTOw OF, I Nov o Is S OSLITe S/N 612.814-61 9CIT CLAGNIFICATIOM OF TNIS

  1. Environmental Impact Statement. Proposed Closure of Los Angeles AFB, California and Relocation of Space Systems Division. Appendix F. Public Comments

    DTIC Science & Technology



  2. Interface Characterization of Cu-Cu and Cu-Ag-Cu Low Temperature Solid State Bonds.

    DTIC Science & Technology


    produce low -. 0 onnos s boniecnstrated, the thrust o: tnis texann a ecne: onaractorlstics as a function c.: 4 time, temperature and pressure . The...conducted under y hyrostatic pressure to avoid deformation of the tensile specimen. At 2Cksi the tensile yield strength of the silver has been exceeded by...A19i 915 INTERFACE CHARACTERIZATION OF CU-CU AND CU-AG-CU LOW 1/1 TEMPERATURE SOLID STATE BONDS(U) NAVAL POSTGRADUATE SCHOO0L MONTEREY CA R Z DALBEY

  3. 2250-MHz High Efficiency Microwave Power Amplifier (HEMPA)

    NASA Technical Reports Server (NTRS)

    Sims, W. Herbert; Bell, Joseph L. (Technical Monitor)


    Tnis paper will focus on developing an exotic switching technique that enhances the DC-to-RF conversion efficiency of microwave power amplifiers. For years, switching techniques implemented in the 10 kHz to 30 MHz region have resulted in DC-to-RF conversion efficiencies of 90-95-percent. Currently amplifier conversion efficiency, in the 2-3 GHz region approaches, 10-20-percent. Using a combination of analytical modeling and hardware testing, a High Efficiency Microwave Power Amplifier was built that demonstrated conversion efficiencies four to five times higher than current state of the art.

  4. Array I Photo Imagery Analysis.

    DTIC Science & Technology


    stereo scan Ssearcn, was -ated 2s oeina: Detectaole i-I - rti iy Detectac’e 1 - ’ ion etectaole .A ’Ile Ŕ" rating means that one is capable of detecting...tures on a ohotograph. Another term "minimum ground seoaration ," :cmes into context for tnis iiscussion. This is the ininimum distance oetween ions sroula be notedi: 1. The brightness of the mine to be resolved; 2. The uniformity of the target background; and 3. The extent of the uniform

  5. Prince George Apt, British Columbia, Canada, Revised Uniform Summary of Surface Weather Observations (RUSSWO). Parts A-F.

    DTIC Science & Technology


    DO NOT REPRODUCE LEGIBLY. SEC,,RITY CASSIFICA ! ON OF Tn-S PAGE REh- -nt,/ . i I REPORT DOCUMENTATION PAGE RE MSTING FORM I REPORT NUMaLA ’ GOVT...standard, deviations, and observation counts of station pressure and sea-level pressure). Data in this report are presented in tabular form , in most cases...DrITOF$ OF tNIS FORM ARE OBSOLETE -" - ,I T ss : II I T;V.2 , 1’ FTE. WEATHER CONDITIONS ". ’ C-.,T A T- STATION STATION NAME YEARS MONTH I. E(-At F

  6. MCNP calculations for container inspection with tagged neutrons

    NASA Astrophysics Data System (ADS)

    Boghen, G.; Donzella, A.; Filippini, V.; Fontana, A.; Lunardon, M.; Moretto, S.; Pesente, S.; Zenoni, A.


    We are developing an innovative tagged neutrons inspection system (TNIS) for cargo containers: the system will allow us to assay the chemical composition of suspect objects, previously identified by a standard X-ray radiography. The operation of the system is extensively being simulated by using the MCNP Monte Carlo code to study different inspection geometries, cargo loads and hidden threat materials. Preliminary simulations evaluating the signal and the signal over background ratio expected as a function of the system parameters are presented. The results for a selection of cases are briefly discussed and demonstrate that the system can operate successfully in different filling conditions.

  7. Lock and Dam 13 Major Rehabilitation Whiteside County, Illinois, Clinton County, Iowa.

    DTIC Science & Technology


    Tnis oermit does not authorize the disposal of crecged material in an Imoortant Resource Area wh-ich has Deen cesionated as such by the illinois...Illinois," approved June 10, 1911, as amenoed. (Illinois Revised Statutes, Cnapter 19, paragraphs 52 et seq.) 2. This oermit does not convey title to, organics 2. Laboratory testing was performed in accordance with EN 1110-2-1906 dated 30 Nov 70, revised 1 Nay 80 and 20 Aug 86. All samples were

  8. Structure and Deformation Characteristics of Rheocast Metals.

    DTIC Science & Technology



  9. Decision Support for Attack Submarine Commanders.

    DTIC Science & Technology



  10. A Strategy for Reforming Avionics Acquisition and Support

    DTIC Science & Technology


    9OS/2-AF S1’ A4-VSM r J. C, Geb ma n. H. L. Shulman ATA 4Qy-r7’-777 SECURITY CLASSIFICATION O. TNIS P&Gg (Whom D & tritedf) REPORT DOCUMENTATION...Range Planning & Doctrine Div, (AF/XOXFP) July 1988 Directorate of Plans, Ofc DCS/Plans & Operations I3. NUMUEROFPAGES Hq USAF, Washington, D . C...avionics subsystems. Results from exploratory applications of the concept to the fire control radars on the F-15 C/ D and the F-16 A/B indicate that the Air

  11. A Laser Trap for Neutral Atoms.

    DTIC Science & Technology


    alexandrite laser operating in DO , 1473 OITIow OFr I NOV ’IS 13 OBSOLETE S/N 0 102- I.F- 0 14- 6601 SECURITY CLASSIFICATION OF TNIS PAGE (WhenOnt...development and characterization of the new alexandrite laser, demonstration of the trap at low densities, and deter- mination of the spatial and...range and the trap depth down by vr). We have used spa- tial filtering to reliably and stabily operate our new cw alexandrite laser as well as argon and

  12. Thermodynamics of High Temperature Materials.

    DTIC Science & Technology


    Department of Commerce 23 -1A , /7 National Bureau of Standards A102 Washington, D.C. 20234 ______________ I I. CONTROLLING OFFICE NAME AND ADDRESS Air...DISTRIBUTION STATEMENT (of this Report) r ~Appro-,’. f’or public re r-: e ; 17. DISTRIBUTION STATEMENT (of the abstract entered in Block 20, It different from...8SOLETE SCRT SEUIYCLASSIFICATION OF TNIS PAGE " e aoEtr AEOST.1-0443 THERMODYNAMICS OF HIGH TEMPERATURE MATERIALS Annual Report for the Period of 1 October

  13. The Toxicity of Nitroguanidine and Photolyzed Nitroguandine to Freshwater Aquatic Organisms

    DTIC Science & Technology


    Paratanytarsus dissimlils), and aquatic worms ( Lumbriculus variegatus ). The. IN) tWS UCLASSIFIED SEURIY4T CLAISIPICAtIOW OF TNIS PAGE (1160 Date U40*4. I. II...2868 0 ( Lumbriculus variegatue ) 1785 0 (1.49 0 a. The high, low, and control NGu concentrations are listed for each test. The top concentration tested...Bailey, H.C. and D.H.W. Liu. 1980. Lumbriculus variegatus , a benthic oligochaete, as a bioassay organism. In: A.L. Buikema and J. Cairns, Jr., eds

  14. The Ecology of the Benthic and Endolithic Communities of a Rocky Reef in the Kelp Beds off Del Monte Beach, Monterey, California.

    DTIC Science & Technology


    RECIPIENT’S CATALOG NUMMER 4. TITLCj~ T.egj max-& ~vRC eocology of the !nthic and %Ser’S hes is,4 .1ndo RLthic ommunities of a lcky eef S EFRIGOG EOTNNE 0 t...1 . CONTRACT OR GRANT mumB90R( s ) 9. PER11FORMING ORGANIZATION NAME ANC ACORESS 10. PROGRAM EL9EMENT. PROJE01CT. TASKC AREA &WORK UNIT MBER’S Naval...COITION OF IMOV goIsSOSL6T7NLSSFE9 ~ ) 41 S /N@10014550 ISECURITY CLASSFICATION OF TNIS PAGE66 IMN Doe ete") UNCLASSIFIED 󈧘. (continued) the level of

  15. Rigid-Flex Printed Circuit Manufacturing process. A Project of the Manufacturing Technology Program.

    DTIC Science & Technology


    002- L -0146601SECURITY CLASSIFICATION OF TNIS PAGE (011ioe Data Bntered) UNCLASSIFIED SUc~hfTY cLAIsaicooricoi OF THIS PACE (IIo "a Mu9.,erva...n I irt I Olut I Av- ’ji L ~ iii1’_ t PREFACE Advances in manufacturing technology are continuing to be made as both new materials and processing...Peel, Laminate Exhibit 10 6 5 4 1 2 3 Cost 5 6 3 4 2 1 L Facility Rating 4 5 2 6 3 1 Indicates Failure/Test Requirements I/ Indicates After Etching T

  16. Identification of MDMB-FUBINACA in commercially available e-liquid formulations sold for use in electronic cigarettes.


    Peace, Michelle R; Krakowiak, Rose I; Wolf, Carl E; Poklis, Alphonse; Poklis, Justin L


    MDMB-FUBINACA (aka MDMB(N)-Bz-F), chemical name Methyl (S)-2-(1-(4-fluorobenzyl)-1H-indazole-3-carboxamido)-3,3-dimethylbutanoate, a designer drug or a new psychoactive substance (NPS), was identified in three commercially available e-liquids formulated for electronic cigarette use. The e-liquids were evaluated using direct analysis in real time ion source attached to a time of flight mass spectrometer (DART-MS) and gas chromatograph mass spectrometer (GC-MS) to identify active ingredients/drugs, flavorants, and other possible constituents. The e-liquids were also evaluated for alcohol content by headspace gas chromatography with flame ionization detector (HS-GC-FID). The aerosol produced from the e-liquids by use of an e-cigarette was analyzed by solid phase micro-extraction gas chromatography mass spectrometry (SPME-GC-MS) to ensure delivery of the active ingredient/drug. Propylene glycol, vegetable glycerin, MDMB-FUBINACA, alcohol content and a flavor profile were determined for each of the e-liquids. MDMB-FUBINACA was determined to be the major active ingredient in all three e-liquids and was successfully detected by SPME-GC-MS in the aerosol generated by a KangerTech Aerotank clearomizer/electronic cigarette.

  17. Computer Program Development Specification for IDAMST Operational Flight Programs. Addendum 1. Executive Software.

    DTIC Science & Technology


    terminal to receive Zris 7,esscgE. 7/R: rL E or ; 0 v z . ,-c this bit is set zne terminal is t3 s . tnis cit .s not set, the terminal is to receive : usL ...are listed in Table lS -I, and an explanation of each one follows. Those Mode Codes that are not defined here may he used by the system...r0 e w araci~e-xr Ifor hi a temp was W z. py zre value contained in the .i~iparametrr * a~ :’~ carcsot tne actual Pd rc u K:- ’aa e LS . ne

  18. Experimental validation of MCNP simulations for the EURITRACK Tagged Neutron Inspection System

    NASA Astrophysics Data System (ADS)

    Donzella, A.; Bodini, I.; Zenoni, A.; Fontana, A.; Perot, B.; Bernard, S.; Carasco, C.; Mariani, A.; Sudac, D.; Valkovic, V.


    The detection of illicit trafficking such as explosive materials inside large commercial payloads is today an important worldwide problem. Differently from the X- or γ-ray based systems, neutron interrogation allows the elemental composition of inspected materials to be measured. A container interrogation device based on the Tagged Neutron Inspection System (TNIS) has been developed within the EURITRACK (EURopean Illicit TRAfficking Countermeasures Kit) project of the 6th Framework Program of the European Union. A prototype of such system has been integrated at the Ruder Boskovic Institute (IRB) of Zagreb, Croatia, and performance tests have been carried out. A detailed simulation of the IRB experimental set-up has been performed. Comparison of the Monte Carlo predictions with collected experimental data is presented.

  19. Irradiation of 4''x4'' NaI(Tl) detector by the 14 MeV neutrons.


    Sudac, D; Valkovic, V


    Within the EURopean Illicit TRAfficking Countermeasures Kit (EURITRACK) project, a new Tagged Neutron Inspection System (TNIS) has been developed and installed in the Port of Rijeka in Croatia. The system was based on the examination of sea containers with the 14 MeV neutron beam. During the operation the characteristic gamma rays were produced and measured by several 5''x5''x10'' NaI(Tl) detectors. During this procedure some of the detectors were exposed to an intensive neutron beam radiation. It was necessary to check for possible radiation damage of the NaI(Tl) scintillator during the gamma detector selection phase of the project. The 4''x4'' NaI(Tl) detector was exposed to 14 MeV neutrons for 20 h. From the presented results on energy resolution and activation measurements it could be concluded that there are no significant differences in energy resolution before and after the irradiation by 4.7x10(11) of 14 MeV neutrons. The only problem could be the high level of medium and long term induced activity in the energy region below 2 MeV.

  20. Formation and stabilization of C6- by radiative electron attachment

    NASA Astrophysics Data System (ADS)

    Chandrasekaran, Vijayanand; Prabhakaran, Aneesh; Kafle, Bhim; Rubinstein, Hilel; Heber, Oded; Rappaport, Michael; Toker, Yoni; Zajfman, Daniel


    Radiative electron attachment (REA) plays an important role in forming molecular anions in various astrophysical environments. In this work, we determined the rate coefficient for the formation of C6- by REA based on a detailed balance approach. C6- ions are stored in an electrostatic ion beam trap and are photoexcited above their adiabatic detachment energy (4.18 eV). Due to fast internal conversion and intramolecular vibrational redistribution, photoexcitation leads to the formation of temporary negative ions (TNIs), the same as those one formed by the electron attachment. Absolute vibrational autodetachment and recurrent (or Poincaré) fluorescence (RF) rate coefficients have already been reported [V. Chandrasekaran et al., J. Phys. Chem. Lett. 5, 4078 (2014)]. Knowing the branching ratios of the various competing rate coefficients is decisive to the understanding of the formation probability of anions via REA. The radiative stabilization rate of C6-, shown to be dominated by RF, was determined to be 5 × 104 s-1 at the electron detachment energy, i.e., at least a factor of 100 faster than the stabilization by infrared transitions. The RF is found to very effectively stabilize the TNI formed by electron attachment. Using detailed balance to link the measured delayed detachment rate to the rate of electron attachment, we estimate the REA rate leading to the formation of C6- to be 3 × 10-7 cm3 s-1 at 300 K in agreement with theory (1.7 × 10-7 cm3 s-1 [R. Terzieva and E. Herbst, Int. J. Mass Spectrom. 201, 135 (2000)]). Such a high rate for REA to C6 indicates that REA may play a prominent role in the formation of anions in the interstellar medium.

  1. Spectroscopic and ITC study of the conformational change upon Ca{sup 2+}-binding in TnC C-lobe and TnI peptide complex from Akazara scallop striated muscle

    SciTech Connect

    Yumoto, Fumiaki; Tanaka, Hiroyuki; Nagata, Koji; Miyauchi, Yumiko; Miyakawa, Takuya; Ojima, Takao; Tanokura, Masaru


    Akazara scallop (Chlamys nipponensis akazara) troponin C (TnC) of striated adductor muscle binds only one Ca{sup 2+} ion at the C-terminal EF-hand motif (Site IV), but it works as the Ca{sup 2+}-dependent regulator in adductor muscle contraction. In addition, the scallop troponin (Tn) has been thought to regulate muscle contraction via activating mechanisms that involve the region spanning from the TnC C-lobe (C-lobe) binding site to the inhibitory region of the TnI, and no alternative binding of the TnI C-terminal region to TnC because of no similarity between second TnC-binding regions of vertebrate and the scallop TnIs. To clarify the Ca{sup 2+}-regulatory mechanism of muscle contraction by scallop Tn, we have analyzed the Ca{sup 2+}-binding properties of the complex of TnC C-lobe and TnI peptide, and their interaction using isothermal titration microcalorimetry, nuclear magnetic resonance, circular dichroism, and gel filtration chromatography. The results showed that single Ca{sup 2+}-binding to the Site IV leads to a structural transition not only in Site IV but also Site III through the structural network in the C-lobe of scallop TnC. We therefore assumed that the effect of Ca{sup 2+}-binding must lead to a change in the interaction mode between the C-lobe of TnC and the TnI peptide. The change should be the first event of the transmission of Ca{sup 2+} signal to TnI in Tn ternary complex.

  2. Cooperative regulation of myosin-S1 binding to actin filaments by a continuous flexible Tm-Tn chain.


    Mijailovich, Srboljub M; Kayser-Herold, Oliver; Li, Xiaochuan; Griffiths, Hugh; Geeves, Michael A


    The regulation of striated muscle contraction involves cooperative interactions between actin filaments, myosin-S1 (S1), tropomyosin (Tm), troponin (Tn), and calcium. These interactions are modeled by treating overlapping tropomyosins as a continuous flexible chain (CFC), weakly confined by electrostatic interactions with actin. The CFC is displaced locally in opposite directions on the actin surface by the binding of either S1 or Troponin I (TnI) to actin. The apparent rate constants for myosin and TnI binding to and detachment from actin are then intrinsically coupled via the CFC model to the presence of neighboring bound S1s and TnIs. Monte Carlo simulations at prescribed values of the CFC stiffness, the CFC's degree of azimuthal confinement, and the angular displacements caused by the bound proteins were able to predict the stopped-flow transients of S1 binding to regulated F-actin. The transients collected over a large range of calcium concentrations could be well described by adjusting a single calcium-dependent parameter, the rate constant of TnI detachment from actin, k(-I). The resulting equilibrium constant K(B) ≡ 1/K(I) varied sigmoidally with the free calcium, increasing from 0.12 at low calcium (pCa >7) to 12 at high calcium (pCa <5.5) with a Hill coefficient of ~2.15. The similarity of the curves for excess-actin and excess-myosin data confirms their allosteric relationship. The spatially explicit calculations confirmed variable sizes for the cooperative units and clustering of bound myosins at low calcium concentrations. Moreover, inclusion of negative cooperativity between myosin units predicted the observed slowing of myosin binding at excess-myosin concentrations.