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Sample records for kuruoglu melek masal

  1. [Microkeratome and excimer laser-assisted endothelial keratoplasty (MELEK)].

    PubMed

    Thannhäuser, C L; Palka, K; Herbst, H; Schroeter, J; Pham, D T

    2014-10-01

    Descemet's stripping automated endothelial keratoplasty (DSAEK) and Descemet membrane endothelial keratoplasty (DMEK) have become well established procedures for the treatment of endothelial pathologies. In the last years the field of lamellar corneal surgery has further developed in terms of preparation of the lamellae as well as of implantation. A modified form of the "ultrathin DSAEK" (UT-DSAEK) is the "microkeratome and excimer laser-assisted endothelial keratoplasty" (MELEK). In this new technique a corneal graft is prepared by a single cut of a microkeratome followed by a stromal excimer-laser thinning and smoothing. The purpose of the present report is to describe this new technique and present first clinical results. In this prospective clinical study 18 patients (76 ± 11 years) underwent a MELEK. The BCVA increased from 0.25 ± 0.1 preoperatively to one month postoperatively was 0.33 ± 0.21 (decimal, n = 12), after three months 0.51 ± 0.23 (n = 8) and after six months 0.80 ± 0.16 (n = 4). The average thickness of the residual stromal lamella before laser ablation was 173 ± 42 µm, after ablation 111 ± 15 µm. The central corneal thickness decreased from 704 µm to 639 µm, the thickness of the transplant decreased from 114 µm to 106 µm six months postoperatively. The ultrathin "microkeratome and excimer laser-assisted endothelial keratoplasty" (MELEK) is a new and safe technique in the field of lamellar keratoplasty. In the future it could have the potential to combine the advantages of DSAEK and DMEK for the treatment of endothelial pathologies. Georg Thieme Verlag KG Stuttgart · New York.

  2. Biological safety assessment of mutant variant of Allium sativum leaf agglutinin (mASAL), a novel antifungal protein for future transgenic application.

    PubMed

    Ghosh, Prithwi; Roy, Amit; Chakraborty, Joydeep; Das, Sampa

    2013-12-04

    Genetic engineering has established itself to be an important tool for crop improvement. Despite the success, there is always a risk of food allergy induced by alien gene products. The present study assessed the biosafety of mutant Allium sativum leaf agglutinin (mASAL), a potent antifungal protein generated by site directed mutagenesis of Allium sativum leaf agglutinin (ASAL). mASAL was cloned in pET28a+ and expressed in E. coli, and the safety assessment was carried out according to the FAO/WHO guideline (2001). Bioinformatics analysis, pepsin digestion, and thermal stability assay showed the protein to be nonallergenic. Targeted sera screening revealed no significant IgE affinity of mASAL. Furthermore, mASAL sensitized Balb/c mice showed normal histopathology of lung and gut tissue. All results indicated the least possibility of mASAL being an allergen. Thus, mASAL appears to be a promising antifungal candidate protein suitable for agronomical biotechnology.

  3. Functional Alteration of a Dimeric Insecticidal Lectin to a Monomeric Antifungal Protein Correlated to Its Oligomeric Status

    PubMed Central

    Banerjee, Nilanjana; Ghosh, Prithwi; Das, Kalipada; Das, Sampa

    2011-01-01

    Background Allium sativum leaf agglutinin (ASAL) is a 25-kDa homodimeric, insecticidal, mannose binding lectin whose subunits are assembled by the C-terminal exchange process. An attempt was made to convert dimeric ASAL into a monomeric form to correlate the relevance of quaternary association of subunits and their functional specificity. Using SWISS-MODEL program a stable monomer was designed by altering five amino acid residues near the C-terminus of ASAL. Methodology/Principal Findings By introduction of 5 site-specific mutations (-DNSNN-), a β turn was incorporated between the 11th and 12th β strands of subunits of ASAL, resulting in a stable monomeric mutant ASAL (mASAL). mASAL was cloned and subsequently purified from a pMAL-c2X system. CD spectroscopic analysis confirmed the conservation of secondary structure in mASAL. Mannose binding assay confirmed that molecular mannose binds efficiently to both mASAL and ASAL. In contrast to ASAL, the hemagglutination activity of purified mASAL against rabbit erythrocytes was lost. An artificial diet bioassay of Lipaphis erysimi with mASAL displayed an insignificant level of insecticidal activity compared to ASAL. Fascinatingly, mASAL exhibited strong antifungal activity against the pathogenic fungi Fusarium oxysporum, Rhizoctonia solani and Alternaria brassicicola in a disc diffusion assay. A propidium iodide uptake assay suggested that the inhibitory activity of mASAL might be associated with the alteration of the membrane permeability of the fungus. Furthermore, a ligand blot assay of the membrane subproteome of R. solani with mASAL detected a glycoprotein receptor having interaction with mASAL. Conclusions/Significance Conversion of ASAL into a stable monomer resulted in antifungal activity. From an evolutionary aspect, these data implied that variable quaternary organization of lectins might be the outcome of defense-related adaptations to diverse situations in plants. Incorporation of mASAL into agronomically

  4. Entrepreneurship--Born, Made and Educated

    ERIC Educational Resources Information Center

    Burger-Helmchen, Thierry, Ed.

    2012-01-01

    Chapters in this book include: (1) The Psychology of Entrepreneurship (Melek Kalkan and Canani Kaygusuz); (2) Entrepreneurial Intentions: The Role of the Cognitive Variables (Jose C. Sanchez); (3) Do External Factors Influence Students' Entrepreneurial Inclination? An Evidence Based Approach (Ishfaq Ahmed, Muhammad Musarrat Nawaz and Muhammad…

  5. Entrepreneurship--Born, Made and Educated

    ERIC Educational Resources Information Center

    Burger-Helmchen, Thierry, Ed.

    2012-01-01

    Chapters in this book include: (1) The Psychology of Entrepreneurship (Melek Kalkan and Canani Kaygusuz); (2) Entrepreneurial Intentions: The Role of the Cognitive Variables (Jose C. Sanchez); (3) Do External Factors Influence Students' Entrepreneurial Inclination? An Evidence Based Approach (Ishfaq Ahmed, Muhammad Musarrat Nawaz and Muhammad…

  6. Exposure to methamphetamine during first and second half of prenatal period and its consequences on cognition after long-term application in adulthood.

    PubMed

    Hrebíčková, I; Malinová-Ševčíková, M; Macúchová, E; Nohejlová, K; Šlamberová, R

    2014-01-01

    It is known that psychostimulants including methamphetamine (MA) have neurotoxic effect, especially, if they are targeting CNS during its critical periods of development. The present study was aimed on evaluation of cognitive changes following scheduled prenatal MA exposure in combination with long-term exposure in adulthood of male rats. Two periods of gestation were targeted: 1(st) half - the embryonic day (ED) 1-11 and 2(nd) half - ED 12-22. Rat mothers received subcutaneously a daily injection of MA (5 mg/kg) or saline (SAL, 1 ml/kg) throughout scheduled periods. Male offspring were tested for cognitive changes in the Morris Water Maze (MWM) in adulthood. Each day of the experiment animals received an injection of MA (1 mg/kg) or SAL (1 ml/kg) during 12 days. Our results demonstrated that in the group of animals exposed to the drug during ED 1-11, neither prenatal MA exposure, nor adult MA treatment changed the performance in the MWM test. Only the velocity was increased in group with long-term MA treatment (SAL/MA and MA/MA). In the group of animals exposed to the drug during ED 12-22, rats exposed to MA prenatally and also in adulthood (MA/MA) swam faster but learned the position of the platform slower in the Place Navigation Test than animals exposed to SAL in adulthood (MA/SAL). In the Probe Test, MA/SAL had decreased velocity and swam shorter distance than MA/MA or SAL/SAL rats suggesting increased floating of these animals. In the Memory Retention Test, SAL/MA rats swam shorter distance than SAL/SAL or MA/MA animals suggesting changes in used strategies in memory recall. As conclusion, our results suggest differences in the effect of combination of prenatal and adult exposure to MA. These effects further depend on the stage of CNS development and schedule of MA exposure affecting intrauterine development in male rats.

  7. Comparing Sensory Information Processing and Alexithymia between People with Substance Dependency and Normal.

    PubMed

    Bashapoor, Sajjad; Hosseini-Kiasari, Seyyedeh Tayebeh; Daneshvar, Somayeh; Kazemi-Taskooh, Zeinab

    2015-01-01

    Sensory information processing and alexithymia are two important factors in determining behavioral reactions. Some studies explain the effect of the sensitivity of sensory processing and alexithymia in the tendency to substance abuse. Giving that, the aim of the current study was to compare the styles of sensory information processing and alexithymia between substance-dependent people and normal ones. The research method was cross-sectional and the statistical population of the current study comprised of all substance-dependent men who are present in substance quitting camps of Masal, Iran, in October 2013 (n = 78). 36 persons were selected randomly by simple randomly sampling method from this population as the study group, and 36 persons were also selected among the normal population in the same way as the comparison group. Both groups was evaluated by using Toronto alexithymia scale (TAS) and adult sensory profile, and the multivariate analysis of variance (MANOVA) test was applied to analyze data. The results showed that there are significance differences between two groups in low registration (P < 0.020, F = 5.66), sensation seeking (P < 0.050, F = 1.92), and sensory avoidance (P < 0.008, F = 7.52) as a components of sensory processing and difficulty in describing emotions (P < 0.001, F = 15.01) and difficulty in identifying emotions (P < 0.002, F = 10.54) as a components of alexithymia. However, no significant difference were found between two groups in components of sensory sensitivity (P < 0.170, F = 1.92) and external oriented thinking style (P < 0.060, F = 3.60). These results showed that substance-dependent people process sensory information in a different way than normal people and show more alexithymia features than them.

  8. Pore-space alteration in source rock (shales) during hydrocarbons generation: X-ray microtomography and pore-scale modelling study

    NASA Astrophysics Data System (ADS)

    Korost, Dmitry; Gerke, Kirill; Akhmanov, Grigory; Vasilyev, Roman; Čapek, Pavel; Karsanina, Marina; Nadezhkin, Dmitry

    2013-04-01

    Hydrocarbons (HC) are generated from solid organic matter (kerogen) due to thermocatalytic reactions. The rate of such reactions shows direct correlation with temperature and depends on the depth of source rock burial. Burial of sedimentary rock is also inevitably accompanied by its structural alteration owing to compaction, dehydration and re-crystallization. Processes of HC generation, primary migration and structural changes are inaccessible for direct observation in nature, but they can be studied in laboratory experiments. Modern technical facilities of laboratories make it possible to carry out experiments on HC generation from the organic-rich rocks at a completely new level (Kobchenko et al., 2011). Some new technologies, including X-ray microtomography and pore-scale modeling, allow us to carry out a step-by-step description of such processes and their development, and to study their reflection in alterations of rock structure. Experiments were carried out with a clayey-carbonate rock sample of the Domanic Formaition taken at a depth of 1939 m from borehole drilled in the central part of the Melekes depression (West Tatar arch, Russia). The rock chosen fits the very essential requirements for studying HC generation under laboratory conditions - high organic matter content and its low metamorphic grade. Our work aimed such a study in an undisturbed rock sample by heating it in nitrogen atmosphere based on a specified temperature regime in a RockEval6 analyzer and monitoring alterations in the pore space structure. Observations were carried out with a SkyScan-1172 X-ray microtomography scanner (resulting scan resolution of 1 µm). A cylinder, 4 mm in diameter, was prepared from the rock sample for the pyrolitic and microtomographic analyses. Scanning procedures were carried out in 5 runs. Temperature interval for each run had to match the most important stage of HC generation in the source rock, namely: (1) original structure; (2) 100-300˚? - discharge of

  9. Fractured Reservoirs and Crustal Fluids in the Precambrian Basement of the Volga-Ural Region

    NASA Astrophysics Data System (ADS)

    Plotnikova, I.

    2009-04-01

    that are substantially different in concentration from those from the uppermost portion of the basement. Their total salinity is 289 g/l. The water-dissolved gas of the upper portion of the basement contains methane and nitrogen. The waters of the crystalline basement are characterised by significant concentrations (by carbon) of the water-dissolved organic matter including phenols, nitric components, etc. Bitumen of the fractured zones of the crystalline basement mainly contains hydrocarbons with C14 to C33, occasionally - with C9. A range of hydrocarbons becomes wider in the zones of cataclasm and mylonization. Geophysical and geological investigations have indicated numerous reservoir zones in crystalline basement bearing the traces of bitumen and fluids enriched with the dissolved hydrocarbon gases. The crystalline rocks studies mainly consisted of the analyses of deep fluids containing the dissolved gases and a monitoring of their composition conducted over several years. As a result, spatial distribution of reservoirs has been determined. The crystalline basement of the South Tatarstan Arch and Melekes trough have been found to contain bitumen with a high uranium content in microfractures (Muslimov & Lapinskaya, 1996). Gas chromatography and polished sections studies have shown that the rocks affected by reducing fluids have a maximum hydrocarbon content. Gas components are mainly represented by methane, its homologs and minor amounts of unsaturated compounds.