Sample records for kuruoglu melek masal

  1. Biological safety assessment of mutant variant of Allium sativum leaf agglutinin (mASAL), a novel antifungal protein for future transgenic application.


    Ghosh, Prithwi; Roy, Amit; Chakraborty, Joydeep; Das, Sampa


    Genetic engineering has established itself to be an important tool for crop improvement. Despite the success, there is always a risk of food allergy induced by alien gene products. The present study assessed the biosafety of mutant Allium sativum leaf agglutinin (mASAL), a potent antifungal protein generated by site directed mutagenesis of Allium sativum leaf agglutinin (ASAL). mASAL was cloned in pET28a+ and expressed in E. coli, and the safety assessment was carried out according to the FAO/WHO guideline (2001). Bioinformatics analysis, pepsin digestion, and thermal stability assay showed the protein to be nonallergenic. Targeted sera screening revealed no significant IgE affinity of mASAL. Furthermore, mASAL sensitized Balb/c mice showed normal histopathology of lung and gut tissue. All results indicated the least possibility of mASAL being an allergen. Thus, mASAL appears to be a promising antifungal candidate protein suitable for agronomical biotechnology.

  2. Entrepreneurship--Born, Made and Educated

    ERIC Educational Resources Information Center

    Burger-Helmchen, Thierry, Ed.


    Chapters in this book include: (1) The Psychology of Entrepreneurship (Melek Kalkan and Canani Kaygusuz); (2) Entrepreneurial Intentions: The Role of the Cognitive Variables (Jose C. Sanchez); (3) Do External Factors Influence Students' Entrepreneurial Inclination? An Evidence Based Approach (Ishfaq Ahmed, Muhammad Musarrat Nawaz and Muhammad…

  3. Fractured Reservoirs and Crustal Fluids in the Precambrian Basement of the Volga-Ural Region

    NASA Astrophysics Data System (ADS)

    Plotnikova, I.


    that are substantially different in concentration from those from the uppermost portion of the basement. Their total salinity is 289 g/l. The water-dissolved gas of the upper portion of the basement contains methane and nitrogen. The waters of the crystalline basement are characterised by significant concentrations (by carbon) of the water-dissolved organic matter including phenols, nitric components, etc. Bitumen of the fractured zones of the crystalline basement mainly contains hydrocarbons with C14 to C33, occasionally - with C9. A range of hydrocarbons becomes wider in the zones of cataclasm and mylonization. Geophysical and geological investigations have indicated numerous reservoir zones in crystalline basement bearing the traces of bitumen and fluids enriched with the dissolved hydrocarbon gases. The crystalline rocks studies mainly consisted of the analyses of deep fluids containing the dissolved gases and a monitoring of their composition conducted over several years. As a result, spatial distribution of reservoirs has been determined. The crystalline basement of the South Tatarstan Arch and Melekes trough have been found to contain bitumen with a high uranium content in microfractures (Muslimov & Lapinskaya, 1996). Gas chromatography and polished sections studies have shown that the rocks affected by reducing fluids have a maximum hydrocarbon content. Gas components are mainly represented by methane, its homologs and minor amounts of unsaturated compounds.

  4. Pore-space alteration in source rock (shales) during hydrocarbons generation: X-ray microtomography and pore-scale modelling study

    NASA Astrophysics Data System (ADS)

    Korost, Dmitry; Gerke, Kirill; Akhmanov, Grigory; Vasilyev, Roman; Čapek, Pavel; Karsanina, Marina; Nadezhkin, Dmitry


    Hydrocarbons (HC) are generated from solid organic matter (kerogen) due to thermocatalytic reactions. The rate of such reactions shows direct correlation with temperature and depends on the depth of source rock burial. Burial of sedimentary rock is also inevitably accompanied by its structural alteration owing to compaction, dehydration and re-crystallization. Processes of HC generation, primary migration and structural changes are inaccessible for direct observation in nature, but they can be studied in laboratory experiments. Modern technical facilities of laboratories make it possible to carry out experiments on HC generation from the organic-rich rocks at a completely new level (Kobchenko et al., 2011). Some new technologies, including X-ray microtomography and pore-scale modeling, allow us to carry out a step-by-step description of such processes and their development, and to study their reflection in alterations of rock structure. Experiments were carried out with a clayey-carbonate rock sample of the Domanic Formaition taken at a depth of 1939 m from borehole drilled in the central part of the Melekes depression (West Tatar arch, Russia). The rock chosen fits the very essential requirements for studying HC generation under laboratory conditions - high organic matter content and its low metamorphic grade. Our work aimed such a study in an undisturbed rock sample by heating it in nitrogen atmosphere based on a specified temperature regime in a RockEval6 analyzer and monitoring alterations in the pore space structure. Observations were carried out with a SkyScan-1172 X-ray microtomography scanner (resulting scan resolution of 1 µm). A cylinder, 4 mm in diameter, was prepared from the rock sample for the pyrolitic and microtomographic analyses. Scanning procedures were carried out in 5 runs. Temperature interval for each run had to match the most important stage of HC generation in the source rock, namely: (1) original structure; (2) 100-300