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Sample records for kuuse picea mariana

  1. Molecular Variation in Picea Rubens and Picea Mariana

    NASA Astrophysics Data System (ADS)

    Bobola, Michael S.

    Restriction fragment length polymorphism (RFLP) variation was examined among samples from the entire range of red spruce (Picea rubens Sarg.), the eastern complex of black spruce (Picea mariana (Mill.) B.S.P.), control -cross red-on-black hybrids, and natural populations of red and black spruce. Within-species and population variation was examined. In addition an accurate species index capable of identifying red spruce, black spruce and hybridization between the two species was developed. The nuclear rDNA repeat unit size in Picea ranged from a minimum of 32 kbp to greater than 40 kbp, two to three fold larger than the typical angiosperm rDNA unit. At a size greater than 32 kbp and a concentration averaging 1.2-1.3 times 10^4 copies per pg genomic DNA, the rDNA repeat constitutes approximately 4% of the spruce genome. The rDNA repeat units were found to be polymorphic within an individual genome with up to five distinct rDNA repeat unit types (alleles) evident. The RFLPs observed in the rDNA repeat were not species specific; however, noticeable trends in internal allelic frequencies were noticed which were useful for between-species differentiation. One marker (EMW 4.35) displayed a significant relationship with geographic origins and habitat suggesting that the observed between-species variation for this marker may be due to selection rather than the result of a true species difference. Variation in the nuclear rDNA repeat could not accurately differentiate hybrids from black spruce. Additional markers were required to identify hybrids. RFLPs were identified for the organelle genomes of red spruce, and black spruce. The organelle inheritance pattern was deduced using controlled-cross hybrids. Organelle markers were combined with allelic data from the nuclear rDNA repeat to derive a simple three character index capable of identifying red spruce, black spruce and hybridization between the two species. Significant gene flow was observed between red and black spruce

  2. A high-density genetic linkage map of a black spruce (Picea mariana) × red spruce (Picea rubens) interspecific hybrid.

    PubMed

    Kang, Bum-Yong; Major, John E; Rajora, Om P

    2011-02-01

    Genetic maps provide an important genomic resource of basic and applied significance. Spruce (Picea) has a very large genome size (between 0.85 × 1010 and 2.4 × 1010 bp; 8.5-24.0 pg/1C, a mean of 17.7 pg/1C ). We have constructed a near-saturated genetic linkage map for an interspecific backcross (BC1) hybrid of black spruce (BS; Picea mariana (Mill.) B.S.P.) and red spruce (RS; Picea rubens Sarg.), using selectively amplified microsatellite polymorphic loci (SAMPL) markers. A total of 2284 SAMPL markers were resolved using 31 SAMPL-MseI selective nucleotide primer combinations. Of these, 1216 SAMPL markers showing Mendelian segregation were mapped, whereas 1068 (46.8%) SAMPL fragments showed segregation distortion at α = 0.05. Maternal, paternal, and consensus maps consistently coalesced into 12 linkage groups, corresponding to the haploid chromosome number (1n = 1x = 12) of 12 in the genus Picea. The maternal BS map consisted of 814 markers distributed over 12 linkage groups, covering 1670 cM, with a mean map distance of 2.1 cM between adjacent markers. The paternal BS × RS map consisted of 773 markers distributed over 12 linkage groups, covering 1563 cM, with a mean map distance of 2.0 cM between adjacent markers. The consensus interspecific hybrid BC1 map consisted of 1216 markers distributed over 12 linkage groups, covering 1865 cM (98% genome coverage), with a mean map distance of 1.5 cM between adjacent markers. The genetic map reported here provides an important genomic resource in Picea, Pinaceae, and conifers.

  3. Soil physical, chemical and gas-flux characterization from Picea mariana stands near Erickson Creek, Alaska

    USGS Publications Warehouse

    O'Donnell, Jonathan A.; Harden, Jennifer W.; Manies, Kristen L.

    2011-01-01

    Fire is a particularly important control on the carbon (C) balance of the boreal forest, and fire-return intervals and fire severity appear to have increased since the late 1900s in North America. In addition to the immediate release of stored C to the atmosphere through organic-matter combustion, fire also modifies soil conditions, possibly affecting C exchange between terrestrial and atmospheric pools for decades after the burn. The effects of fire on ecosystem C dynamics vary across the landscape, with topographic position and soil drainage functioning as important controls. The data reported here contributed to a larger U.S. Geological Survey (USGS) study, published in the journal Ecosystems by O'Donnell and others (2009). To evaluate the effects of fire and drainage on ecosystem C dynamics, we selected sample sites within the 2003 Erickson Creek fire scar to measure CO2 fluxes and soil C inventories in burned and unburned (control) sites in both upland and lowland black spruce (Picea mariana) forests. The results of this study suggested that although fire can create soil climate conditions which are more conducive to rapid decomposition, rates of C release from soils may be constrained after fire by changes in moisture and (or) substrate quality that impede rates of decomposition. Here, we report detailed site information, methodology, and data (in spreadsheet files) from that study.

  4. Development of highly reliable in silico SNP resource and genotyping assay from exome capture and sequencing: an example from black spruce (Picea mariana).

    PubMed

    Pavy, Nathalie; Gagnon, France; Deschênes, Astrid; Boyle, Brian; Beaulieu, Jean; Bousquet, Jean

    2016-03-01

    Picea mariana is a widely distributed boreal conifer across Canada and the subject of advanced breeding programmes for which population genomics and genomic selection approaches are being developed. Targeted sequencing was achieved after capturing P. mariana exome with probes designed from the sequenced transcriptome of Picea glauca, a distant relative. A high capture efficiency of 75.9% was reached although spruce has a complex and large genome including gene sequences interspersed by some long introns. The results confirmed the relevance of using probes from congeneric species to perform successfully interspecific exome capture in the genus Picea. A bioinformatics pipeline was developed including stringent criteria that helped detect a set of 97,075 highly reliable in silico SNPs. These SNPs were distributed across 14,909 genes. Part of an Infinium iSelect array was used to estimate the rate of true positives by validating 4267 of the predicted in silico SNPs by genotyping trees from P. mariana populations. The true positive rate was 96.2% for in silico SNPs, compared to a genotyping success rate of 96.7% for a set 1115 P. mariana control SNPs recycled from previous genotyping arrays. These results indicate the high success rate of the genotyping array and the relevance of the selection criteria used to delineate the new P. mariana in silico SNP resource. Furthermore, in silico SNPs were generally of medium to high frequency in natural populations, thus providing high informative value for future population genomics applications.

  5. Comparative genome mapping among Picea glauca, P. mariana x P. rubens and P. abies, and correspondence with other Pinaceae.

    PubMed

    Pelgas, Betty; Beauseigle, Stéphanie; Acheré, Virginie; Jeandroz, Sylvain; Bousquet, Jean; Isabel, Nathalie

    2006-11-01

    A composite linkage map was constructed from four individual maps for the conifer Picea glauca (Moench) Voss, from anonymous and gene-specific markfers (714 AFLPs, 38 SSRs, and 53 ESTPs). A total of 12 linkage groups were delineated with an average marker density of 2.7 cM. Macro-synteny and macro-colinearity comparisons with two other composite linkage maps developed for the species complex P. mariana (Mill.) B.S.P. x P. rubens Sarg., and for P. abies (L.) Karst. revealed an identical number of linkage groups and a remarkable conservation of the gene content and gene order of linkage groups over the million years since the split between these taxa. Identical gene order among taxa was observed for 10 of the 12 assembled composite linkage groups. The discovery of one breakdown in synteny between P. glauca and the other two taxa indicated the occurrence of an inter-chromosomal rearrangement involving an insertional translocation. Analysis of marker colinearity also revealed a putative segmental duplication. The combined information from these three Picea genomes validated and improved large-scale genome comparisons at the inter-generic level in the family Pinaceae by allowing for the identification of 11 homoeologous linkage groups between Picea and Pinus, and nine such groups between Picea and Pseudotsuga menziesii. Notably, the analysis of synteny among the three genera revealed a putative case of chromosomal fission and an inter-chromosomal rearrangement in the genome of P. menziesii. Both of these changes are inter-connected, indicating much instability in this part of the P. menziesii genome. Overall, the macro-structure of the Pinaceae genome was well conserved, which is notable given the Cretaceous origin of its main lineages.

  6. Effects of NaCl on responses of ectomycorrhizal black spruce (Picea mariana), white spruce (Picea glauca) and jack pine (Pinus banksiana) to fluoride.

    PubMed

    Calvo-Polanco, Mónica; Zwiazek, Janusz J; Jones, Melanie D; MacKinnon, Michael D

    2009-01-01

    Black spruce (Picea mariana), white spruce (Picea glauca) and jack pine (Pinus banksiana) were inoculated with Suillus tomentosus and subjected to potassium fluoride (1 mM KF and 5 mM KF) in the presence and absence of 60 mM NaCl. The NaCl and KF treatments reduced total dry weights in jack pine and black spruce seedlings, but they did not affect total dry weights in white spruce seedlings. The addition of 60 mM NaCl to KF treatment solutions alleviated fluoride-induced needle injury in ectomycorrhizal (ECM) black spruce and white spruce, but had little effect in jack pine seedlings. Both KF and 60 mM NaCl treatments reduced E values compared with non-treated control seedlings. However, with the exception of small reductions of K(r) by NaCl treatments in black spruce, the applied KF and NaCl treatments had little effect on K(r) in ECM plants. Chloride tissue concentrations in NaCl-treated plants were not affected by the presence of KF in treatment solutions. However, shoot F concentrations in ECM black spruce and white spruce treated with 5 mM KF + 60 mM NaCl were significantly reduced compared with the 5 mM KF treatment. The results point to a possible competitive inhibition of F transport by Cl. We also suggest that the possibility that aquaporins may be involved in the transmembrane transport of F should be further investigated.

  7. Needle age and season influence photosynthetic temperature response and total annual carbon uptake in mature Picea mariana trees

    PubMed Central

    Jensen, Anna M.; Warren, Jeffrey M.; Hanson, Paul J.; Childs, Joanne; Wullschleger, Stan D.

    2015-01-01

    Background and Aims The carbon (C) balance of boreal terrestrial ecosystems is sensitive to increasing temperature, but the direction and thresholds of responses are uncertain. Annual C uptake in Picea and other evergreen boreal conifers is dependent on seasonal- and cohort-specific photosynthetic and respiratory temperature response functions, so this study examined the physiological significance of maintaining multiple foliar cohorts for Picea mariana trees within an ombrotrophic bog ecosystem in Minnesota, USA. Methods Measurements were taken on multiple cohorts of needles for photosynthetic capacity, foliar respiration (Rd) and leaf biochemistry and morphology of mature trees from April to October over 4 years. The results were applied to a simple model of canopy photosynthesis in order to simulate annual C uptake by cohort age under ambient and elevated temperature scenarios. Key Results Temperature responses of key photosynthetic parameters [i.e. light-saturated rate of CO2 assimilation (Asat), rate of Rubisco carboxylation (Vcmax) and electron transport rate (Jmax)] were dependent on season and generally less responsive in the developing current-year (Y0) needles compared with 1-year-old (Y1) or 2-year-old (Y2) foliage. Temperature optimums ranged from 18·7 to 23·7, 31·3 to 38·3 and 28·7 to 36·7 °C for Asat, Vcmax and Jmax, respectively. Foliar cohorts differed in their morphology and photosynthetic capacity, which resulted in 64 % of modelled annual stand C uptake from Y1&2 cohorts (LAI 0·67 m2 m−2) and just 36 % from Y0 cohorts (LAI 0·52 m2 m−2). Under warmer climate change scenarios, the contribution of Y0 cohorts was even less; e.g. 31 % of annual C uptake for a modelled 9 °C rise in mean summer temperatures. Results suggest that net annual C uptake by P. mariana could increase under elevated temperature, and become more dependent on older foliar cohorts. Conclusions Collectively, this study illustrates the physiological and

  8. Generation, functional annotation and comparative analysis of black spruce (Picea mariana) ESTs: an important conifer genomic resource

    PubMed Central

    2013-01-01

    Background EST (expressed sequence tag) sequences and their annotation provide a highly valuable resource for gene discovery, genome sequence annotation, and other genomics studies that can be applied in genetics, breeding and conservation programs for non-model organisms. Conifers are long-lived plants that are ecologically and economically important globally, and have a large genome size. Black spruce (Picea mariana), is a transcontinental species of the North American boreal and temperate forests. However, there are limited transcriptomic and genomic resources for this species. The primary objective of our study was to develop a black spruce transcriptomic resource to facilitate on-going functional genomics projects related to growth and adaptation to climate change. Results We conducted bidirectional sequencing of cDNA clones from a standard cDNA library constructed from black spruce needle tissues. We obtained 4,594 high quality (2,455 5' end and 2,139 3' end) sequence reads, with an average read-length of 532 bp. Clustering and assembly of ESTs resulted in 2,731 unique sequences, consisting of 2,234 singletons and 497 contigs. Approximately two-thirds (63%) of unique sequences were functionally annotated. Genes involved in 36 molecular functions and 90 biological processes were discovered, including 24 putative transcription factors and 232 genes involved in photosynthesis. Most abundantly expressed transcripts were associated with photosynthesis, growth factors, stress and disease response, and transcription factors. A total of 216 full-length genes were identified. About 18% (493) of the transcripts were novel, representing an important addition to the Genbank EST database (dbEST). Fifty-seven di-, tri-, tetra- and penta-nucleotide simple sequence repeats were identified. Conclusions We have developed the first high quality EST resource for black spruce and identified 493 novel transcripts, which may be species-specific related to life history and

  9. The role of soil drainage class in carbon dioxide exchange and decomposition in boreal black spruce (Picea mariana) forest stands

    USGS Publications Warehouse

    Wickland, K.P.; Neff, J.C.; Harden, J.W.

    2010-01-01

    Black spruce (Picea mariana (Mill.) B.S.P.) forest stands range from well drained to poorly drained, typically contain large amounts of soil organic carbon (SOC), and are often underlain by permafrost. To better understand the role of soil drainage class in carbon dioxide (CO2) exchange and decomposition, we measured soil respiration and net CO2 fluxes, litter decomposition and litterfall rates, and SOC stocks above permafrost in three Alaska black spruce forest stands characterized as well drained (WD), moderately drained (MD), and poorly drained (PD). Soil respiration and net CO2 fluxes were not significantly different among sites, although the relation between soil respiration rate and temperature varied with site (Qw: WD > MD > PD). Annual estimated soil respiration, litter decomposition, and groundcover photosynthesis were greatest at PD. These results suggest that soil temperature and moisture conditions in shallow organic horizon soils at PD were more favorable for decomposition compared with the better drained sites. SOC stocks, however, increase from WD to MD to PD such that surface decomposition and C storage are diametric. Greater groundcover vegetation productivity, protection of deep SOC by permafrost and anoxic conditions, and differences in fire return interval and (or) severity at PD counteract the relatively high near-surface decomposition rates, resulting in high net C accumulation.

  10. Effects of temperature and water deficit on cambial activity and woody ring features in Picea mariana saplings.

    PubMed

    Balducci, Lorena; Deslauriers, Annie; Giovannelli, Alessio; Rossi, Sergio; Rathgeber, Cyrille B K

    2013-10-01

    Increase in temperature under the projected future climate change would affect tree growth, including the physiological mechanisms related to sapling responses, which has been examined recently. The study investigated the plant water relations, cambial activity and wood formation in black spruce saplings [Picea mariana (Mill.) B.S.P.] subjected to water deficit and warming. Four-year-old saplings growing in three greenhouses were submitted to different thermal conditions: T0, with a temperature equal to the external air temperature; and T + 2 and T + 5, with temperatures set at 2 and 5 K higher than T0, respectively. We also submitted saplings to two irrigation regimes and studied the effects of a water deficit of 32 days in May-June. We evaluated plant water relations, cambial activity, wood formation and anatomical characteristics from May to October 2010. Lower needle physiology rates were observed during water deficit, with 20-day suspension of irrigation, but after re-watering, non-irrigated saplings attained the same values as irrigated ones in all thermal conditions. Significant differences between irrigation regimes were detected in cambial activity at the end of the water deficit and after resumption of irrigation. Under warmer conditions, the recovery of non-irrigated saplings was slower than T0 and they needed from 2 to 4 weeks to completely restore cambial activity. No significant differences in wood anatomy were observed between irrigation regimes, but there was a sporadic effect on wood density under warming. During wood formation, the warmer conditions combined with water deficit increased sapling mortality by 5 and 12.2% for T + 2 and T + 5, respectively. The black spruce saplings that survived were more sensitive to water availability, and the restoration of cambial activity was slower at temperatures higher than T0. Our results suggest that black spruce showed a plastic response to intense water deficit under warming, but this would compromise

  11. Needle age and season influence photosynthetic temperature response and total annual carbon uptake in mature Picea mariana trees

    SciTech Connect

    Jensen, Anna M.; Warren, Jeffrey; Hanson, Paul J.; Childs, Joanne; Wullschleger, Stan D.

    2015-01-01

    Using seasonal- and cohort-specific photosynthetic temperature response functions, we quantified the physiological significance of maintaining multiple foliar cohorts in mature (~40-45 year old) Picea mariana trees in an ombrotrophic Sphagnum-bog, northern Minnesota, USA. We measured photosynthetic capacity, foliar respiration (Rd), biochemistry and morphology to estimate annual carbon (C) uptake by cohort, season and canopy position. Temperature response of key photosynthetic parameters at 25 C (i.e., light-saturated rate of CO2 assimilation (Asat), light-saturated rate of Rubisco carboxylation (Vcmax), light-saturated electron transport rate (Jmax)) were clearly dependent on season and were generally less responsive in younger needles. Temperature optimums range between 18.7-23.7, 31.3-38.3 and 28.7-36.7 C for Asat, Vcmax and Jmax respectively. Current-year (Y0) foliage had lower photosynthetic capacities compared to one-year-old (Y1) and two-year-old (Y2) foliage. As Y0 needles matured, values of Asat, Vcmax, Jmax, foliar LMA and nitrogen increased. Values of Vcmax, Jmax and Rd were related to foliar nitrogen but only in the youngest (Y0) cohort. Foliar ontogeny affected photosynthetic capacity more than growth temperature. Morphological and physiological cohort differences were reflected by their annual contribution to modeled C uptake, with a ~36% lower estimated annual C uptake by Y0 needles (LAI 0.52 m2m-2) compared to Y1&2 cohorts (LAI 0.67 m2m-2). Collectively, these results illustrate the physiological and ecological significance of characterizing multiple foliar cohorts during bud break and throughout the growth season, and for cumulative C uptake model estimates.

  12. Needle age and season influence photosynthetic temperature response and total annual carbon uptake in mature Picea mariana trees

    DOE PAGES

    Jensen, Anna M.; Warren, Jeffrey; Hanson, Paul J.; ...

    2015-01-01

    Using seasonal- and cohort-specific photosynthetic temperature response functions, we quantified the physiological significance of maintaining multiple foliar cohorts in mature (~40-45 year old) Picea mariana trees in an ombrotrophic Sphagnum-bog, northern Minnesota, USA. We measured photosynthetic capacity, foliar respiration (Rd), biochemistry and morphology to estimate annual carbon (C) uptake by cohort, season and canopy position. Temperature response of key photosynthetic parameters at 25 C (i.e., light-saturated rate of CO2 assimilation (Asat), light-saturated rate of Rubisco carboxylation (Vcmax), light-saturated electron transport rate (Jmax)) were clearly dependent on season and were generally less responsive in younger needles. Temperature optimums range between 18.7-23.7,more » 31.3-38.3 and 28.7-36.7 C for Asat, Vcmax and Jmax respectively. Current-year (Y0) foliage had lower photosynthetic capacities compared to one-year-old (Y1) and two-year-old (Y2) foliage. As Y0 needles matured, values of Asat, Vcmax, Jmax, foliar LMA and nitrogen increased. Values of Vcmax, Jmax and Rd were related to foliar nitrogen but only in the youngest (Y0) cohort. Foliar ontogeny affected photosynthetic capacity more than growth temperature. Morphological and physiological cohort differences were reflected by their annual contribution to modeled C uptake, with a ~36% lower estimated annual C uptake by Y0 needles (LAI 0.52 m2m-2) compared to Y1&2 cohorts (LAI 0.67 m2m-2). Collectively, these results illustrate the physiological and ecological significance of characterizing multiple foliar cohorts during bud break and throughout the growth season, and for cumulative C uptake model estimates.« less

  13. Dynamics at the treeline: differential responses of Picea mariana and Larix laricina to climate change in eastern subarctic Québec

    NASA Astrophysics Data System (ADS)

    Dufour-Tremblay, Geneviève; Lévesque, Esther; Boudreau, Stéphane

    2012-12-01

    Treelines are known to be temperature-sensitive ecotones, and therefore could rapidly expand their range limits in response to climate warming. Observations of lack of range expansion, however, indicate that ecological constraints partly control the treeline ecotones. The main objectives of this study were to evaluate Picea mariana and Larix laricina recruitment and growth at and above the altitudinal treeline of Kangiqsualujjuaq (Nunavik), where warmer temperatures since the 1990s have already triggered shrub expansion. We mapped, harvested, dated and measured tree individuals along two altitudinal gradients from the forested stands below the treeline to hilltops. Since the 1990s, a pulse of L. laricina seedling establishment has occurred at and above the treeline. Dendrochronological analysis revealed that L. laricina underwent a rapid vertical growth and radial growth that accelerated from the 1990s. No recruitment was observed for P. mariana in response to the regional warming, suggesting a regeneration failure of this species. Our results indicated that the L. laricina colonization below and above the treeline in recent decades in response to the regional warming should modify the landscape physiognomy of the study area in the near future.

  14. Tracking the progression of speciation: variable patterns of introgression across the genome provide insights on the species delimitation between progenitor-derivative spruces (Picea mariana × P. rubens).

    PubMed

    de Lafontaine, Guillaume; Prunier, Julien; Gérardi, Sébastien; Bousquet, Jean

    2015-10-01

    The genic species concept implies that while most of the genome can be exchanged somewhat freely between species through introgression, some genomic regions remain impermeable to interspecific gene flow. Hence, interspecific differences can be maintained despite ongoing gene exchange within contact zones. This study assessed the heterogeneous patterns of introgression at gene loci across the hybrid zone of an incipient progenitor-derivative species pair, Picea mariana (black spruce) and Picea rubens (red spruce). The spruce taxa likely diverged in geographic isolation during the Pleistocene and came into secondary contact during late Holocene. A total of 300 SNPs distributed across the 12 linkage groups (LG) of black spruce were genotyped for 385 individual trees from 33 populations distributed across the allopatric zone of each species and within the zone of sympatry. An integrative framework combining three population genomic approaches was used to scan the genomes, revealing heterogeneous patterns of introgression. A total of 23 SNPs scattered over 10 LG were considered impermeable to introgression and putatively under diverging selection. These loci revealed the existence of impermeable genomic regions forming the species boundary and are thus indicative of ongoing speciation between these two genetic lineages. Another 238 SNPs reflected selectively neutral diffusion across the porous species barrier. Finally, 39 highly permeable SNPs suggested ancestral polymorphism along with balancing selection. The heterogeneous patterns of introgression across the genome indicated that the speciation process between black spruce and red spruce is young and incomplete, albeit some interspecific differences are maintained, allowing ongoing species divergence even in sympatry. The approach developed in this study can be used to track the progression of ongoing speciation processes.

  15. Sequestration of soil nitrogen as tannin-protein complexes may improve the competitive ability of sheep laurel (Kalmia angustifolia) relative to black spruce (Picea mariana).

    PubMed

    Joanisse, G D; Bradley, R L; Preston, C M; Bending, G D

    2009-01-01

    The role of litter tannins in controlling soil nitrogen (N) cycling may explain the competitive ability of Kalmia relative to black spruce (Picea mariana), although this has not been demonstrated experimentally. Here, the protein-precipitation capacities of purified tannins and leaf extracts from Kalmia and black spruce were compared. The resistance to degradation of tannin-protein precipitates from both species were compared by monitoring carbon (C) and N dynamics in humus amended with protein, purified tannins or protein-tannin precipitates. The purity of the precipitates was verified using solid-state (13)C nuclear magnetic resonance (NMR) spectra. The ability of mycorrhizal fungi associated with both species to grow on media amended with tannin-protein complexes as the principal N source was also compared. The protein precipitation capacity of Kalmia tannins was superior to those of black spruce. Humus amended with protein increased both mineral and microbial N, whereas humus amended with tannin-protein precipitates increased dissolved organic N. Mycorrhizal fungi associated with Kalmia showed better growth than those associated with black spruce when N was provided as tannin-protein precipitates. These data suggest that Kalmia litter increases the amount of soil N sequestered as tannin-protein complexes, which may improve the competitive ability of Kalmia relative to black spruce by favouring N uptake by mycorrhizas associated with the former.

  16. Characterization of an AGAMOUS homologue from the conifer black spruce (Picea mariana) that produces floral homeotic conversions when expressed in Arabidopsis.

    PubMed

    Rutledge, R; Regan, S; Nicolas, O; Fobert, P; Côté, C; Bosnich, W; Kauffeldt, C; Sunohara, G; Séguin, A; Stewart, D

    1998-09-01

    Advances in elucidating the molecular processes controlling flower initiation and development have provided unique opportunities to investigate the developmental genetics of non-flowering plants. In addition to providing insights into the evolutionary aspects of seed plants, identification of genes regulating reproductive organ development in gymnosperms could help determine the level of homology with current models of flower induction and floral organ identity. Based upon this, we have searched for putative developmental regulators in conifers with amino acid sequence homology to MADS-box genes. PCR cloning using degenerate primers targeted to the MADS-box domain revealed the presence of over 27 MADS-box genes within black spruce (Picea mariana), including several with extensive homology to either AP1 or AGAMOUS, both known to regulate flower development in Arabidopsis. This indicates that like angiosperms, conifers contain a large and diverse MADS-box gene family that probably includes regulators of reproductive organ development. Confirmation of this was provided by the characterization of an AGAMOUS-like cDNA clone called SAG1, whose conservation of intron position and tissue-specific expression within reproductive organs indicate that it is a homologue of AGAMOUS. Functional homology with AGAMOUS was demonstrated by the ability of SAG1 to produce homeotic conversions of sepals to carpels and petals to stamens when ectopically expressed in transgenic Arabidopsis. This suggests that some of the genetic pathways controlling flower and cone development are homologous, and antedate the 300-million-year-old divergence of angiosperms and gymnosperms.

  17. Will changes in root-zone temperature in boreal spring affect recovery of photosynthesis in Picea mariana and Populus tremuloides in a future climate?

    PubMed

    Fréchette, Emmanuelle; Ensminger, Ingo; Bergeron, Yves; Gessler, Arthur; Berninger, Frank

    2011-11-01

    Future climate will alter the soil cover of mosses and snow depths in the boreal forests of eastern Canada. In field manipulation experiments, we assessed the effects of varying moss and snow depths on the physiology of black spruce (Picea -mariana (Mill.) B.S.P.) and trembling aspen (Populus tremuloides Michx.) in the boreal black spruce forest of western Québec. For 1 year, naturally regenerated 10-year-old spruce and aspen were grown with one of the following treatments: additional N fertilization, addition of sphagnum moss cover, removal of mosses, delayed soil thawing through snow and hay addition, or accelerated soil thawing through springtime snow removal. Treatments that involved the addition of insulating moss or snow in the spring caused lower soil temperature, while removing moss and snow in the spring caused elevated soil temperature and thus had a warming effect. Soil warming treatments were associated with greater temperature variability. Additional soil cover, whether moss or snow, increased the rate of photosynthetic recovery in the spring. Moss and snow removal, on the other hand, had the opposite effect and lowered photosynthetic activity, especially in spruce. Maximal electron transport rate (ETR(max)) was, for spruce, 39.5% lower after moss removal than with moss addition, and 16.3% lower with accelerated thawing than with delayed thawing. Impaired photosynthetic recovery in the absence of insulating moss or snow covers was associated with lower foliar N concentrations. Both species were affected in that way, but trembling aspen generally reacted less strongly to all treatments. Our results indicate that a clear negative response of black spruce to changes in root-zone temperature should be anticipated in a future climate. Reduced moss cover and snow depth could adversely affect the photosynthetic capacities of black spruce, while having only minor effects on trembling aspen.

  18. The postglacial history of three Picea species in New England, USA

    NASA Astrophysics Data System (ADS)

    Lindbladh, Matts; Jacobson, George L.; Schauffler, Molly

    2003-01-01

    Given the difficulty of separating the three Picea species— P. glauca, P. mariana, and P. rubens (white, black, and red spruce)—in the pollen record, little is known about their unique histories in eastern North America following deglaciation. Here we report the first use of a classification tree analysis (CART) to distinguish pollen grains of these species. It was successfully applied to fossil pollen from eight sites in Maine and one in Massachusetts. We focused on the late glacial/early Holocene (14,000 to 8000 cal yr B.P.) and the late Holocene (1400 cal yr B.P. to present)—the two key periods since deglaciation when Picea has been abundant in the region. The result shows a shift from a Picea forest of P. glauca and P. mariana in the late glacial to a forest of P. rubens and P. mariana in the late Holocene. The small number of P. rubens grains identified from the late glacial/early Holocene samples (<5%) suggests that that species was either absent or rare at most of the sites. The occurrence and distribution of the three species do not reveal any geographic or temporal trend during late glacial time, but the data suggest that they were distributed in local patches on the landscape. The results of this study indicate that the recent population expansion of Picea (1000 to 500 cal yr B.P.) was likely the first time since deglaciation that P. rubens was abundant in the region.

  19. Nuclear ribosomal DNA internal transcribed spacer 1 (ITS1) in Picea (Pinaceae): sequence divergence and structure.

    PubMed

    Campbell, Christopher S; Wright, Wesley A; Cox, Margaret; Vining, Thomas F; Major, C Smoot; Arsenault, Matthew P

    2005-04-01

    The nrDNA ITS1 of Picea is 2747-3271 bp, the longest known of all plants. We obtained 24 cloned ITS1 sequences from six individuals of Picea glehnii, Picea mariana, Picea orientalis, and Picea rubens. Mean sequence divergence within these individuals (0.018+/-0.009) is more than half that between the species (0.031+/-0.011) and may be maintained against concerted evolution by separation of Picea 18S-26S rDNA repeats on multiple chromosomes. Picea ITS1 contains three subrepeats with a motif (5'-GGCCACCCTAGTC) that is conserved across Pinaceae. Two subrepeats are tandem, remote from the third, and more closely related and significantly more similar to one another than either is to the third subrepeat. This correlation between similarity and proximity may be the result of subrepeat duplication or concerted evolution within rDNA repeats. In inferred secondary structures, subrepeats generally form long hairpins, with a portion of the Pinaceae conserved motif in the terminal loop, and tandem subrepeats pair with one another over most of their length. Coalescence of ITS1 sequences occurs in P. orientalis but not in the other species.

  20. Holocene occurrence of Lophodermium piceae, a black spruce needle endophyte and possible paleoindicator of boreal forest health

    NASA Astrophysics Data System (ADS)

    Jasinski, J. P. Paul; Payette, Serge

    2007-01-01

    Holocene occurrences of conifer needle endophytes have not previously been reported. We report the fossil remains of Lophodermium piceae (Fckl.) Hoehn., a fungal endophyte of black spruce ( Picea mariana (Mill.) B.S.P.) needles, in macrofossils dating back to 8000 cal yr BP. Spruce budworm head capsules and L. piceae remains were found preceding charcoal layers delineating the transformation of four spruce-moss forest sites to spruce-lichen woodland. As L. piceae is found solely on senescent needles, its increased presence during these transformation periods likely indicates that the forests were in decline due to the spruce budworm ( Choristoneura fumiferana (Clem.)) when they burned. Future paleoecological studies incorporating needle fungi observations could be used to investigate the historical occurrence of tree disease and the role of fungi in forest health and decline.

  1. Anatahan Volcano, Mariana Islands

    NASA Technical Reports Server (NTRS)

    2008-01-01

    In the early hours of February 7, ASTER captured this nighttime thermal infrared image of an eruption of Anatahan Volcano in the central Mariana Islands. The summit of the volcano is bright indicating there is a very hot area there. Streaming to the west is an ash plume, visible by the red color indicating the presence of silicate-rich particles. Dark grey areas are clouds that appear colder than the ocean. Anatahan is a stratovolcano that started erupting in May 2003, forming a new crater.

    The image covers an area of 56.3 x 41.8 km, and is located 16 degrees north latitude and 145.6 degrees east longitude.

    The U.S. science team is located at NASA's Jet Propulsion Laboratory, Pasadena, Calif. The Terra mission is part of NASA's Science Mission Directorate.

  2. 40 CFR 81.354 - Northern Mariana Islands.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 18 2014-07-01 2014-07-01 false Northern Mariana Islands. 81.354... § 81.354 Northern Mariana Islands. Northern Mariana Islands—TSP Designated area Does not meet primary... 1 X 1 EPA designation only. Northern Mariana Islands—1971 Sulfur Dioxide NAAQS (Primary...

  3. 40 CFR 81.354 - Northern Mariana Islands.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 18 2012-07-01 2012-07-01 false Northern Mariana Islands. 81.354... § 81.354 Northern Mariana Islands. Northern Mariana Islands—TSP Designated area Does not meet primary... 1 X 1 EPA designation only. Northern Mariana Islands—SO2 Designated area Does not meet...

  4. 40 CFR 81.354 - Northern Mariana Islands.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 18 2013-07-01 2013-07-01 false Northern Mariana Islands. 81.354... § 81.354 Northern Mariana Islands. Northern Mariana Islands—TSP Designated area Does not meet primary... 1 X 1 EPA designation only. Northern Mariana Islands—SO2 Designated area Does not meet...

  5. 40 CFR 81.354 - Northern Mariana Islands.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 17 2010-07-01 2010-07-01 false Northern Mariana Islands. 81.354... § 81.354 Northern Mariana Islands. Northern Mariana Islands—TSP Designated area Does not meet primary... 1 X 1 EPA designation only. Northern Mariana Islands—SO2 Designated area Does not meet...

  6. 40 CFR 81.354 - Northern Mariana Islands.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 17 2011-07-01 2011-07-01 false Northern Mariana Islands. 81.354... § 81.354 Northern Mariana Islands. Northern Mariana Islands—TSP Designated area Does not meet primary... 1 X 1 EPA designation only. Northern Mariana Islands—SO2 Designated area Does not meet...

  7. Growth dynamics of black spruce (Picea mariana) in a rapidly thawing discontinuous permafrost peatland

    NASA Astrophysics Data System (ADS)

    Sniderhan, Anastasia E.; Baltzer, Jennifer L.

    2016-12-01

    High-latitude warming has led to radical changes in abiotic conditions influencing forest growth. In the North American boreal forest, widespread declines in forest productivity (particularly in western regions) and changing climate-growth relationships have been documented. Previous studies have proposed that this decline can be attributed to drought stress as increasing temperatures may cause evapotranspirative demand to exceed available moisture. We used tree ring studies to document growth dynamics of black spruce, one of the most dominant boreal tree species, in a boreal peatland experiencing rapid permafrost thaw. We specifically look at how changing permafrost conditions influence growth. Growth of black spruce at this site has declined steadily since the mid-1900s and exhibited a shift from positive responses to temperature pre-1970 to predominantly negative responses in recent decades, despite precipitation increasing over time at this site. Our results show that there is no apparent effect of landscape position or rate of lateral permafrost thaw on growth trends of black spruce, despite gradients in soil moisture and active layer thickness across the mosaic of wetlands and drier permafrost plateaus at this site. However, this does not imply no effect of permafrost thaw on growth; our results support growing evidence that vertical permafrost thaw (i.e., active layer thickening) is causing drought stress in these slow-growing, shallow-rooted trees. To our knowledge, this study is the first to investigate permafrost as a driver of within-site variability in growth-climate responses, and we provide insight into the widespread growth declines and divergence of climate-growth relationships in high-latitude forests.

  8. Soil data from different-age Picea mariana stands near Delta Junction, Alaska

    USGS Publications Warehouse

    Manies, Kristen L.; Harden, Jennifer W.

    2011-01-01

    One objective of the U.S. Geological Survey\\'s Fate of Carbon in Alaskan Landscapes (FOCAL) project is to study the effects of fire and soil drainage on soil carbon storage in boreal forests. For this purpose, the project has measured the soil carbon content in several chronosequences (time since disturbance) of various soil-drainage types. One such chronosequence near Delta Junction, Alaska was initially studied in 2000 and 2001. Additional sites in the Delta Junction area were sampled in 2006 to expand the number of stand ages represented in the chronosequence. This report describes these additional sites, as well as the procedures used to describe, sample, and analyze the soils. We also present data tables containing, but not limited to, field descriptions, bulk density, moisture content, and total carbon (C) and total nitrogen (N) content.

  9. Composition and Biological Activity of Picea pungens and Picea orientalis Seed and Cone Essential Oils.

    PubMed

    Wajs-Bonikowska, Anna; Szoka, Łukasz; Karna, Ewa; Wiktorowska-Owczarek, Anna; Sienkiewicz, Monika

    2017-03-01

    The increasing consumption of natural products lead us to discover and study new plant materials, such as conifer seeds and cones, which could be easily available from the forest industry as a waste material, for their potential uses. The chemical composition of the essential oils of Picea pungens and Picea orientalis was fully characterized by GC and GC/MS methods. Seed and cone oils of both tree species were composed mainly of monoterpene hydrocarbons, among which limonene, α- and β-pinene were the major, but in different proportions in the examined conifer essential oils. The levorotary form of chiral monoterpene molecules was predominant over the dextrorotary form. The composition of oils from P. pungens seeds and cones was similar, while the hydrodistilled oils of P. orientalis seeds and cones differed from each other, mainly by a higher amount of oxygenated derivatives of monoterpenes and by other higher molar mass terpenes in seed oil. The essential oils showed mild antimicrobial action, however P. orientalis cone oil exhibited stronger antimicrobial properties against tested bacterial species than those of P. pungens. Effects of the tested cone essential oils on human skin fibroblasts and microvascular endothelial cells (HMEC-1) were similar: in a concentration of 0 - 0.075 μl/ml the oils were rather safe for human skin fibroblasts and 0 - 0.005 μl/ml for HMEC-1 cells. IC50 value of Picea pungens oils was 0.115 μl/ml, while that of Picea orientalis was 0.105 μl/ml. The value of IC50 of both oils were 0.035 μl/ml for HMEC-1 cells. The strongest effect on cell viability had the oil from Picea orientalis cones, while on DNA synthesis the oil from Picea pungens cones.

  10. Crustal structure of the Mariana Trough

    SciTech Connect

    Ambos, E.L.; Hussong, D.M.

    1982-05-10

    Three seismic refraction profiles were shot in the Mariana Trough in the vicinity of the proposed axial rift spreading center. One long east-west trending line was shot across the axial high region, the elevated portion of the trough associated with the rift zone. The two other lines were shot in a rough north-south orientation subparallel to the trend of the axial rift, on crusts 1 and 5--6 m.y. old. Comparison of the results of the ray-tracing analysis for these two profiles indicated that thinning of layer 2A (velocity of 3.3 km/s) and development of a distinct layer 2B (velocity of 5.3 km/s and development of a distinct layer 2B (velocity of 5.3 km/s) and layer 3A (velocity of 6.7 km/s) takes place with increasing crustal age, implying a process of crustal evolution. The presence of large-scale crustal faults was noted in several instances. In addition, a consistent but low upper mantle seismic velocity of 7.7 km/s was measured for all three lines. These characteristics correlate with those previously observed for other areas of young oceanic crust. When compared with major active spreading centers such as the Mid-Atlantic Ridge and East Pacific Rise, the Mariana Trough appears similar to the former in most respects. Both the Mid-Atlantic Ridge and Mariana Trough are slow spreading (1-2 cm/yr half rate) and are characterized by rough topography, an axial rift, and a thick layer/sup 2/. Like the Mid-Atlantic Ridge, the Mariana Trough also has no low-velocity zone in the crust that could be interpreted as a magma chamber at the spreading center.

  11. 50 CFR 665.440 - Mariana crustacean fisheries. [Reserved

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 50 Wildlife and Fisheries 13 2013-10-01 2013-10-01 false Mariana crustacean fisheries. 665.440 Section 665.440 Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL OCEANIC AND... Archipelago Fisheries § 665.440 Mariana crustacean fisheries....

  12. 50 CFR 665.440 - Mariana crustacean fisheries. [Reserved

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 50 Wildlife and Fisheries 13 2012-10-01 2012-10-01 false Mariana crustacean fisheries. 665.440 Section 665.440 Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL OCEANIC AND... Archipelago Fisheries § 665.440 Mariana crustacean fisheries....

  13. 50 CFR 665.440 - Mariana crustacean fisheries. [Reserved

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 50 Wildlife and Fisheries 13 2014-10-01 2014-10-01 false Mariana crustacean fisheries. 665.440 Section 665.440 Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL OCEANIC AND... Archipelago Fisheries § 665.440 Mariana crustacean fisheries....

  14. 50 CFR 665.440 - Mariana crustacean fisheries. [Reserved

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 50 Wildlife and Fisheries 9 2010-10-01 2010-10-01 false Mariana crustacean fisheries. 665.440 Section 665.440 Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL OCEANIC AND... Archipelago Fisheries § 665.440 Mariana crustacean fisheries....

  15. 50 CFR 665.440 - Mariana crustacean fisheries. [Reserved

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 50 Wildlife and Fisheries 11 2011-10-01 2011-10-01 false Mariana crustacean fisheries. 665.440 Section 665.440 Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL OCEANIC AND... Archipelago Fisheries § 665.440 Mariana crustacean fisheries....

  16. 8 CFR 235.9 - Northern Marianas identification card.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 8 Aliens and Nationality 1 2013-01-01 2013-01-01 false Northern Marianas identification card. 235.9 Section 235.9 Aliens and Nationality DEPARTMENT OF HOMELAND SECURITY IMMIGRATION REGULATIONS... Northern Mariana Islands in Political Union with the United States entered into force on November 3,...

  17. Soil data from Picea mariana stands near delta junction, Alaska of different ages and soil drainage type

    USGS Publications Warehouse

    Manies, Kristen L.; Harden, Jennifer W.; Silva, Steven R.; Briggs, Paul H.; Schmid, Brian M.

    2004-01-01

    The U.S. Geological Survey project Fate of Carbon in Alaskan Landscapes (FOCAL) is studying the effect of fire and soil drainage on soil carbon storage in the boreal forest. This project has selected several sites to study within central Alaska of varying ages (time since fire) and soil drainage types. This report describes the location of these sampling sites, as well as the procedures used to describe, sample, and analyze the soils. This report also contains data tables with this information, including, but not limited to field descriptions, bulk density, particle size distribution, moisture content, carbon (C) concentration, nitrogen (N) concentration, isotopic data for C, and major, minor and trace elemental concentration.

  18. Miocene reef carbonates of Mariana Islands

    SciTech Connect

    Siegrist, H.G. Jr.

    1988-01-01

    Miocene carbonates in the southern Mariana Islands are impressive for their lithologic diversity, thicknesses (over 250 m), and geographic extend (>20% combined outcrop coverage over four major high islands: Guam, Rota, Tinian and Saipan). Sections are dominated either by lagoonal algal-foraminiferal wackestones and mudstones with locally abundant high-energy shelly-skeletal facies, or by rubbly to muddy, fore-reef-to-bank deposits of packstones and grainstones with highly diverse and variable biogenic clasts. Fresh to deeply weathered volcaniclastic material may comprise at least 80% of some high-energy fore-reef facies, whereas lagoonal and bank deposits usually contain less than 0.5% terrigenous material. Surprisingly, the Miocene in the Marianas lacks almost completely any reef-core facies. Several poorly developed coral-rich mounds on Saipan and localized laminated red algal buildups on Guam appear to constitute the extant reef-wall facies in the Miocene. The lack of buildups may be a matter of differential survival; it may result from headland erosion and benching associated with emergency of narrow reef tracts as has been postulated by others for south Guam. Radiometric age dating of these reef carbonates has proven unsuccessful because pervasive diagenesis has transformed the entire Miocene section into low-magnesium calcite with minor and occasional dolomite. Freshwater phreatic diagenesis accounts for the principal porosity variation and trace element distribution.

  19. Miocene reef carbonates of Mariana Islands

    SciTech Connect

    Siegrist, H.G. Jr.

    1988-02-01

    Miocene carbonates in the southern Mariana Islands are impressive for their lithologic diversity, thicknesses (over 250 m), and geographic extent (> 20% combined outcrop coverage over four major high islands: Guam, Rota, Tinian and Saipan). Sections are dominated either by lagoonal algal-foraminiferal wackestones and mudstones with locally abundant high-energy shelly-skeletal facies, or by rubbly to muddy, fore-reef-to-bank deposits of packstones and grainstones with highly diverse and variable biogenic clasts. Fresh to deeply weathered volcaniclastic material may comprise at least 80% of some high-energy fore-reef facies, whereas lagoonal and bank deposits usually contain less than 0.5% terrigenous material. Surprisingly, the Miocene in the Marianas lacks almost completely any reef-core facies. Several poorly developed coral-rich mounds on Saipan and localized laminated red algal buildups on Guam appear to constitute the extant reef-wall facies in the Miocene. The lack of buildups may be a matter of differential survival; it may result from headland erosion and benching associated with emergence of narrow reef tracts as has been postulated by others for south Guam. Alternatively, the authors are proposing that Miocene bathymetry and the volume of terrigenous influx militated against significant reef core formation. Radiometric age dating of these reef carbonates has proven unsuccessful because pervasive diagenesis has transformed the entire Miocene section into low-magnesium calcite with minor and occasional dolomite. Freshwater phreatic diagenesis accounts for the principal porosity variation and trace element distribution.

  20. Population size and natural history of Mariana fruit bats (Chiroptera: Pteropodidae) on Sarigan, Mariana Islands

    USGS Publications Warehouse

    Wiles, G.J.; Jonhson, N.C.

    2004-01-01

    Based on count results, we estimated the population of Mariana fruit bats (Pteropus mariannus Desmarest) on Sarigan, Mariana Islands, to number 150-200 bats in 1999, 185-235 bats in 2000, and about 300-400 bats in 2001. Our results, plus those of two previous surveys, indicate that bat abundance on the island probably remained relatively stable at about 125-235 animals during much of the period from 1983 to 2000, then increased suddenly in 2001, most likely due to immigration from a neighboring island. Sarigan's population differs from those of larger islands in the archipelago by usually having smaller roost sizes, typically 3-75 bats, and large numbers of solitary bats that at times comprise up to half of the population. Colonies and smaller aggregations were composed primarily of harems with multiple females, whereas a nearly equal sex ratio occurred among solitary animals. Colonies roosted in isolated coconut trees in open grasslands and in native forest stands of various sizes, but avoided dense coconut forest. An estimated 30-50% of harem and solitary females possessed young in July 1999. Bats were recorded feeding on just six species of plants, which partly reflects the island's impoverished flora. We speculate that fruit bat abundance on Sarigan is limited primarily by food availability rather than hunting losses, in contrast to some other islands in the Marianas. Our study supports the contention that populations of P. mariannus in the northern Marianas are usually sedentary, but that interisland movements of larger numbers of bats may occur rarely. ?? 2004 by University of Hawai'i Press All rights reserved.

  1. Population trends of Mariana Crow Corvus kubaryi on Rota, Commonwealth of the Northern Mariana Islands

    USGS Publications Warehouse

    Plentovich, S.; Morton, J.M.; Bart, J.; Camp, R.J.; Lusk, M.; Johnson, N.; VanderWerf, E.

    2005-01-01

    Endemic to the islands of Guam and Rota in the Mariana Islands, Mariana Crow Corvus kubaryi is the only corvid in Micronesia. Currently, it survives on Guam only because of translocation of individuals from Rota (1999-2003). Island-wide surveys in 1982 and 1995 on Rota yielded population estimates of 1,348 and 592 respectively, indicating a 56% decrease in only 13 years. A sharp decline in the only viable Mariana Crow population has serious implications for conservation efforts on Rota and for efforts to re-establish the Guam population. However, the validity of the apparent decline has been debated among scientists and government management agencies. We augmented the 1982 and 1995 island-wide VCP surveys with (1) an additional island-wide survey conducted in 1998, and (2) roadside surveys conducted during 1991-1993 and again during 1999-2002. We also outline historical changes in Rota's limestone forest based on aerial photographs and historical information. Data from all surveys indicate a significant decline in the Mariana Crow population. Declines occurred especially along the north-central coast and in the area east of the airport known as As Dudo in the 1990s, but the data indicate an island-wide decline over the entire span of the surveys. introduced predators, human persecution, and habitat loss and degradation by anthropogenic and natural causes have all contributed to the decline. Long-term preservation of this species will require effective brown treesnake Boiga irregularis control, habitat protection, continued monitoring and research, and increased public education and awareness of Rota's rare and endangered species. ?? BirdLife International 2005.

  2. Sedimentary, volcanic, and tectonic processes of the central Mariana Arc: Mariana Trough back-arc basin formation and the West Mariana Ridge

    NASA Astrophysics Data System (ADS)

    Oakley, A. J.; Taylor, B.; Moore, G. F.; Goodliffe, A.

    2009-08-01

    We present new multichannel seismic profiles and bathymetric data from the central Marianas that image the West Mariana Ridge (WMR) remnant arc, both margins of the Mariana Trough back-arc basin, the modern arc, and Eocene frontal-arc high. These data reveal structure and stratigraphy related to three periods of arc volcanism and two periods of arc rifting. We interpret the boundary between accreted back-arc basin and rifted arc crust along the Mariana Trough and support these findings with drilling results and recent seismic refraction and gravity studies. We show that with the exception of a few volcanoes behind the volcanic front that straddle the boundary between crustal types, the modern Mariana Arc is built entirely on rifted arc crust between 14 and 19°N. Our data indicate that there is more accreted back-arc seafloor to the west of the Mariana Trough spreading axis than to the east, confirming previous evidence for an asymmetric basin. The rifted margin of the WMR remnant arc forms a stepped pattern along the western boundary of the Mariana Trough, between 15°30' and 19°N. In this region, linear volcanic cross chains behind the WMR are aligned with the trend of Mariana Trough spreading segments, and the WMR ridges extend into the back-arc basin along the same strike. These ridges are magmatic accommodation zones which, to the north along the Izu-Bonin Arc, punctuate tectonic extension. For the WMR we hypothesize that rift basins are more commonly the sites where spreading segment offsets nucleate, whereas magmatic centers of spreading segments are sites where magmatism continues from arc volcanism, through rifting to back-arc spreading. The Mariana Trough is opening nonrigidly and is characterized by two predominant abyssal hill trends, NNW-SSE in the north and N-S in the south. Between the only two basin-crossing fracture zones at ˜15.5 and 17.5°, N-S axes propagated north at the expense of NNW axes.

  3. 50 CFR 665.420 - Mariana coral reef ecosystem fisheries. [Reserved

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 50 Wildlife and Fisheries 13 2014-10-01 2014-10-01 false Mariana coral reef ecosystem fisheries. 665.420 Section 665.420 Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL OCEANIC... Mariana Archipelago Fisheries § 665.420 Mariana coral reef ecosystem fisheries....

  4. 50 CFR 665.420 - Mariana coral reef ecosystem fisheries. [Reserved

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 50 Wildlife and Fisheries 13 2012-10-01 2012-10-01 false Mariana coral reef ecosystem fisheries. 665.420 Section 665.420 Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL OCEANIC... Mariana Archipelago Fisheries § 665.420 Mariana coral reef ecosystem fisheries....

  5. 50 CFR 665.420 - Mariana coral reef ecosystem fisheries. [Reserved

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 50 Wildlife and Fisheries 11 2011-10-01 2011-10-01 false Mariana coral reef ecosystem fisheries. 665.420 Section 665.420 Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL OCEANIC... Mariana Archipelago Fisheries § 665.420 Mariana coral reef ecosystem fisheries....

  6. 50 CFR 665.420 - Mariana coral reef ecosystem fisheries. [Reserved

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 50 Wildlife and Fisheries 13 2013-10-01 2013-10-01 false Mariana coral reef ecosystem fisheries. 665.420 Section 665.420 Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL OCEANIC... Mariana Archipelago Fisheries § 665.420 Mariana coral reef ecosystem fisheries....

  7. 76 FR 69119 - Commonwealth of the Northern Mariana Islands Transitional Worker Classification: Correction

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-08

    ... SECURITY 8 CFR Part 103 RIN 1615-AB76 Commonwealth of the Northern Mariana Islands Transitional Worker... inadvertently deleted in a September 7, 2011, final rule entitled Commonwealth of the Northern Mariana Islands... the final rule Commonwealth of the Northern Mariana Islands Transitional Worker...

  8. 50 CFR 665.420 - Mariana coral reef ecosystem fisheries. [Reserved

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 50 Wildlife and Fisheries 9 2010-10-01 2010-10-01 false Mariana coral reef ecosystem fisheries. 665.420 Section 665.420 Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL OCEANIC... Mariana Archipelago Fisheries § 665.420 Mariana coral reef ecosystem fisheries....

  9. Interspecific Plastome Recombination Reflects Ancient Reticulate Evolution in Picea (Pinaceae).

    PubMed

    Sullivan, Alexis R; Schiffthaler, Bastian; Thompson, Stacey Lee; Street, Nathaniel R; Wang, Xiao-Ru

    2017-04-05

    Plastid sequences are a cornerstone in plant systematic studies and key aspects of their evolution, such as uniparental inheritance and absent recombination, are often treated as axioms. While exceptions to these assumptions can profoundly influence evolutionary inference, detecting them can require extensive sampling, abundant sequence data, and detailed testing. Using advancements in high-throughput sequencing, we analyzed the whole plastomes of 65 accessions of Picea, a genus of ∼35 coniferous forest tree species, to test for deviations from canonical plastome evolution. Using complementary hypothesis and data-driven tests, we found evidence for chimeric plastomes generated by interspecific hybridization and recombination in the clade comprising Norway spruce (P. abies) and 10 other species. Support for interspecific recombination remained after controlling for sequence saturation, positive selection, and potential alignment artifacts. These results reconcile previous conflicting plastid-based phylogenies and strengthen the mounting evidence of reticulate evolution in Picea. Given the relatively high frequency of hybridization and biparental plastid inheritance in plants, we suggest interspecific plastome recombination may be more widespread than currently appreciated and could underlie reported cases of discordant plastid phylogenies.

  10. Adaptation and exogenous selection in a Picea glauca × Picea engelmannii hybrid zone: implications for forest management under climate change.

    PubMed

    De La Torre, Amanda R; Wang, Tongli; Jaquish, Barry; Aitken, Sally N

    2014-01-01

    The nature of selection responsible for the maintenance of the economically and ecologically important Picea glauca × Picea engelmannii hybrid zone was investigated. Genomic, phenotypic and climatic data were used to test assumptions of hybrid zone maintenance and to model future scenarios under climate change. Genome-wide estimates of admixture based on a panel of 86 candidate gene single nucleotide polymorphisms were combined with long-term quantitative data on growth and survival (over 20 yr), as well as one-time assessments of bud burst and bud set phenology, and cold hardiness traits. A total of 15,498 individuals were phenotyped for growth and survival. Our results suggest that the P. glauca × P. engelmannii hybrid zone is maintained by local adaptation to growing season length and snowpack (exogenous selection). Hybrids appeared to be fitter than pure species in intermediate environments, which fits expectations of the bounded hybrid superiority model of hybrid zone maintenance. Adaptive introgression from parental species has probably contributed to increased hybrid fitness in intermediate habitats. While P. engelmannii ancestry is higher than P. glauca ancestry in hybrid populations, on average, selective breeding in managed hybrid populations is shifting genomic composition towards P. glauca, potentially pre-adapting managed populations to warmer climates.

  11. Adaptation and exogenous selection in a Picea glauca × Picea engelmannii hybrid zone: implications for forest management under climate change

    PubMed Central

    De La Torre, Amanda R; Wang, Tongli; Jaquish, Barry; Aitken, Sally N

    2014-01-01

    The nature of selection responsible for the maintenance of the economically and ecologically important Picea glauca × Picea engelmannii hybrid zone was investigated. Genomic, phenotypic and climatic data were used to test assumptions of hybrid zone maintenance and to model future scenarios under climate change. Genome-wide estimates of admixture based on a panel of 86 candidate gene single nucleotide polymorphisms were combined with long-term quantitative data on growth and survival (over 20 yr), as well as one-time assessments of bud burst and bud set phenology, and cold hardiness traits. A total of 15 498 individuals were phenotyped for growth and survival. Our results suggest that the P. glauca × P. engelmannii hybrid zone is maintained by local adaptation to growing season length and snowpack (exogenous selection). Hybrids appeared to be fitter than pure species in intermediate environments, which fits expectations of the bounded hybrid superiority model of hybrid zone maintenance. Adaptive introgression from parental species has probably contributed to increased hybrid fitness in intermediate habitats. While P. engelmannii ancestry is higher than P. glauca ancestry in hybrid populations, on average, selective breeding in managed hybrid populations is shifting genomic composition towards P. glauca, potentially pre-adapting managed populations to warmer climates. PMID:24200028

  12. Origin and demographic history of the endemic Taiwan spruce (Picea morrisonicola).

    PubMed

    Bodare, Sofia; Stocks, Michael; Yang, Jeng-Chuann; Lascoux, Martin

    2013-09-01

    Taiwan spruce (Picea morrisonicola) is a vulnerable conifer species endemic to the island of Taiwan. A warming climate and competition from subtropical tree species has limited the range of Taiwan spruce to the higher altitudes of the island. Using seeds sampled from an area in the central mountain range of Taiwan, 15 nuclear loci were sequenced in order to measure genetic variation and to assess the long-term genetic stability of the species. Genetic diversity is low and comparable to other spruce species with limited ranges such as Picea breweriana, Picea chihuahuana, and Picea schrenkiana. Importantly, analysis using approximate Bayesian computation (ABC) provides evidence for a drastic decline in the effective population size approximately 0.3-0.5 million years ago (mya). We used simulations to show that this is unlikely to be a false-positive result due to the limited sample used here. To investigate the phylogenetic origin of Taiwan spruce, additional sequencing was performed in the Chinese spruce Picea wilsonii and combined with previously published data for three other mainland China species, Picea purpurea, Picea likiangensis, and P. schrenkiana. Analysis of population structure revealed that P. morrisonicola clusters most closely with P. wilsonii, and coalescent analyses using the program MIMAR dated the split to 4-8 mya, coincidental to the formation of Taiwan. Considering the population decrease that occurred after the split, however, led to a much more recent origin.

  13. 8 CFR 1235.9 - Northern Marianas identification card.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 8 Aliens and Nationality 1 2012-01-01 2012-01-01 false Northern Marianas identification card. 1235.9 Section 1235.9 Aliens and Nationality EXECUTIVE OFFICE FOR IMMIGRATION REVIEW, DEPARTMENT OF... Identification Cards to aliens who acquired United States citizenship when the Covenant to Establish...

  14. 8 CFR 235.9 - Northern Marianas identification card.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 8 Aliens and Nationality 1 2012-01-01 2012-01-01 false Northern Marianas identification card. 235.9 Section 235.9 Aliens and Nationality DEPARTMENT OF HOMELAND SECURITY IMMIGRATION REGULATIONS... aliens who acquired United States citizenship when the Covenant to Establish a Commonwealth of...

  15. 50 CFR 665.400 - Mariana bottomfish fisheries. [Reserved

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 50 Wildlife and Fisheries 9 2010-10-01 2010-10-01 false Mariana bottomfish fisheries. 665.400 Section 665.400 Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE (CONTINUED) FISHERIES IN THE WESTERN PACIFIC...

  16. 50 CFR 665.460 - Mariana precious coral fisheries. [Reserved

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 50 Wildlife and Fisheries 9 2010-10-01 2010-10-01 false Mariana precious coral fisheries. 665.460 Section 665.460 Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE (CONTINUED) FISHERIES IN THE WESTERN PACIFIC...

  17. Observing the Historic Eruption of Northern Mariana Islands Volcano

    NASA Astrophysics Data System (ADS)

    Wiens, Douglas A.; Shore, Patrick J.; Sauter, Allan; Hilton, David R.; Fischer, Tobias; Camacho, Juan T.

    2004-01-01

    Anatahan volcano erupted for the first time in recorded history at about 7:30 GMT on 10 May 2003, covering the island of Anatahan, in the Commonwealth of the Northern Mariana Islands (CNMI), with ash, and providing scientists with important opportunities to study this volcano. The eruption was first reported by the National Oceanic and Atmospheric Administration's Volcanic Ash Advisory Center at 12:32 GMT, based on satellite images of the ash cloud. At about the same time, unusual light flares were observed from an approaching small ship, the Super Emerald, which was carrying a group of seismologists from Washington University in St. Louis, Scripps Institution of Oceanography, and the CNMI Emergency Management Office. As morning broke, the ship was approximately 10 km from the island, and those on board witnessed billowing ash and gas rise from the volcano's caldera to form a great cloud exceeding 6 km in altitude (Figure 1). The scientists were in the region installing land seismographs for the Mariana Subduction Factory Imaging Experiment, a joint U.S.-Japanese deployment of 20 land broadband seismographs and 58 ocean bottom seismographs funded (on the U.S. side) by the Margins program of the National Science Foundation. The experiment has the goal of imaging the magma production regions and mantle flow patterns within the upper mantle beneath the Mariana arc and backarc (see http://epsc.wustl.edu/seismology/MARIANA).

  18. 8 CFR 1235.9 - Northern Marianas identification card.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 8 Aliens and Nationality 1 2013-01-01 2013-01-01 false Northern Marianas identification card. 1235.9 Section 1235.9 Aliens and Nationality EXECUTIVE OFFICE FOR IMMIGRATION REVIEW, DEPARTMENT OF... November 3, 1986. These cards remain valid as evidence of United States citizenship. Although the...

  19. Preliminary Geologic Map of Mount Pagan Volcano, Pagan Island, Commonwealth of the Northern Mariana Islands

    USGS Publications Warehouse

    Trusdell, Frank A.; Moore, Richard B.; Sako, Maurice K.

    2006-01-01

    Pagan Island is the subaerial portion of two adjoining Quaternary stratovolcanoes near the middle of the active Mariana Arc, [FAT1]north of Saipan. Pagan and the other volcanic islands that constitute part of the Arc form the northern half of the East Mariana Ridge[FAT2], which extends about 2-4 km above the ocean floor. The > 6-km-deep Mariana Trench adjoins the East Mariana Ridge on the east, and the Mariana Trough, partly filled with young lava flows and volcaniclastic sediment, lies on the west of the Northern Mariana Islands (East Mariana Ridge. The submarine West Mariana Ridge, Tertiary in age, bounds the western side of the Mariana Trough. The Mariana Trench and Northern Mariana Islands (East Mariana Ridge) overlie an active subduction zone where the Pacific Plate, moving northwest at about 10.3 cm/year, is passing beneath the Philippine Plate, moving west-northwest at 6.8 cm/year. Beneath the Northern Mariana Islands, earthquake hypocenters at depths of 50-250 km identify the location of the west-dipping subduction zone, which farther west becomes nearly vertical and extends to 700 km depth. During the past century, more than 40 earthquakes of magnitude 6.5-8.1 have shaken the Mariana Trench. The Mariana Islands form two sub-parallel, concentric, concave-west arcs. The southern islands comprise the outer arc and extend north from Guam to Farallon de Medinilla. They consist of Eocene to Miocene volcanic rocks and uplifted Tertiary and Quaternary limestone. The nine northern islands extend from Anatahan to Farallon de Pajaros and form part of the inner arc. The active inner arc extends south from Anatahan, where volcanoes, some of which are active, form seamounts west of the older outer arc. Other volcanic seamounts of the active arc surmount the East Mariana Ridge in the vicinity of Anatahan and Sarigan and north and south of Farallon de Pajaros. Six volcanoes (Farallon de Pajaros, Asuncion, Agrigan, Mount Pagan, Guguan, and Anatahan) in the northern islands

  20. 78 FR 39583 - Fisheries in the Western Pacific; Fishing in the Marianas Trench, Pacific Remote Islands, and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-02

    ... Pacific; Fishing in the Marianas Trench, Pacific Remote Islands, and Rose Atoll Marine National Monuments... reports to fish in the Marianas Trench, Pacific Remote Islands, and Rose Atoll Marine National...

  1. Stilbene biosynthesis in the needles of spruce Picea jezoensis.

    PubMed

    Kiselev, K V; Grigorchuk, V P; Ogneva, Z V; Suprun, A R; Dubrovina, A S

    2016-11-01

    Stilbenes are valuable phenolic compounds that are synthesized in plants via the phenylpropanoid pathway where stilbene synthase (STS) directly catalyzes resveratrol or pinosylvin formation. Currently, there is a lack of information about the stilbene biosynthetic pathway in spruce (Picea). Resveratrol and piceatannol derivatives have been detected in the spruce bark, needles, and roots. We analyzed seasonal variation in stilbene spectrum and content in the needles of different ages of one tree of spruce Picea jezoensis. HPLC analysis revealed the presence of nine stilbenes: t- and cis-astringin, t- and cis-piceid, t- and cis-isorhapontin, and t-piceatannol were present in amounts of 0.01-6.07 mg/g of dry weight (DW), while t-isorhapontigenin and t-resveratrol were present in traces (0.001-0.312 μg/g DW). T-astringin prevailed over other stilbenoid compounds (66-86% of all stilbenes). The highest total stilbene content was detected in one-year-old needles collected in the autumn and spring (5.4-7.77 mg/g DW). We previously cloned and sequenced full-length cDNAs of the four STS transcripts (PjSTS1a, PjSTS1b, PjSTS2, and PjSTS3) of P. jezoensis. This study presents a detailed analysis of seasonal variations in PjSTS1a, 1b, 2, and 3 transcript levels in the needles of P. jezoensis of different ages using qRT-PCR. PjSTS1a and PjSTS1b transcription was higher in the needles collected in the autumn, spring, or summer than in the winter. PjSTS2 was actively transcribed in the needles of all ages collected in the winter, spring, and summer. PjSTS3 expression did not significantly change during the year and did not depend on the age of the needles. Therefore, the data show that high levels of the stilbene glucosides and PjSTS expression are present in the needles of P. jezoensis.

  2. 75 FR 15723 - Endangered and Threatened Wildlife and Plants; Draft Revised Recovery Plan for the Mariana Fruit...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-30

    ... for the Mariana Fruit Bat or Fanihi (Pteropus mariannus mariannus) AGENCY: Fish and Wildlife Service... Plan for the Mariana Fruit Bat or Fanihi (Pteropus mariannus mariannus), for public review and comment.... Individual responses to comments will not be provided. This subspecies of the Mariana fruit bat or...

  3. Organellar Genomes of White Spruce (Picea glauca): Assembly and Annotation.

    PubMed

    Jackman, Shaun D; Warren, René L; Gibb, Ewan A; Vandervalk, Benjamin P; Mohamadi, Hamid; Chu, Justin; Raymond, Anthony; Pleasance, Stephen; Coope, Robin; Wildung, Mark R; Ritland, Carol E; Bousquet, Jean; Jones, Steven J M; Bohlmann, Joerg; Birol, Inanç

    2015-12-08

    The genome sequences of the plastid and mitochondrion of white spruce (Picea glauca) were assembled from whole-genome shotgun sequencing data using ABySS. The sequencing data contained reads from both the nuclear and organellar genomes, and reads of the organellar genomes were abundant in the data as each cell harbors hundreds of mitochondria and plastids. Hence, assembly of the 123-kb plastid and 5.9-Mb mitochondrial genomes were accomplished by analyzing data sets primarily representing low coverage of the nuclear genome. The assembled organellar genomes were annotated for their coding genes, ribosomal RNA, and transfer RNA. Transcript abundances of the mitochondrial genes were quantified in three developmental tissues and five mature tissues using data from RNA-seq experiments. C-to-U RNA editing was observed in the majority of mitochondrial genes, and in four genes, editing events were noted to modify ACG codons to create cryptic AUG start codons. The informatics methodology presented in this study should prove useful to assemble organellar genomes of other plant species using whole-genome shotgun sequencing data.

  4. Organellar Genomes of White Spruce (Picea glauca): Assembly and Annotation

    PubMed Central

    Jackman, Shaun D.; Warren, René L.; Gibb, Ewan A.; Vandervalk, Benjamin P.; Mohamadi, Hamid; Chu, Justin; Raymond, Anthony; Pleasance, Stephen; Coope, Robin; Wildung, Mark R.; Ritland, Carol E.; Bousquet, Jean; Jones, Steven J. M.; Bohlmann, Joerg; Birol, Inanç

    2016-01-01

    The genome sequences of the plastid and mitochondrion of white spruce (Picea glauca) were assembled from whole-genome shotgun sequencing data using ABySS. The sequencing data contained reads from both the nuclear and organellar genomes, and reads of the organellar genomes were abundant in the data as each cell harbors hundreds of mitochondria and plastids. Hence, assembly of the 123-kb plastid and 5.9-Mb mitochondrial genomes were accomplished by analyzing data sets primarily representing low coverage of the nuclear genome. The assembled organellar genomes were annotated for their coding genes, ribosomal RNA, and transfer RNA. Transcript abundances of the mitochondrial genes were quantified in three developmental tissues and five mature tissues using data from RNA-seq experiments. C-to-U RNA editing was observed in the majority of mitochondrial genes, and in four genes, editing events were noted to modify ACG codons to create cryptic AUG start codons. The informatics methodology presented in this study should prove useful to assemble organellar genomes of other plant species using whole-genome shotgun sequencing data. PMID:26645680

  5. Forest bird and fruit bat populations on Sarigan, Mariana Islands

    USGS Publications Warehouse

    Fancy, Steven G.; Craig, Robert J.; Kessler, Curt T.

    1999-01-01

    We conducted the first quantitative surveys of forest bird and bat populations on the uninhabited island of Sarigan, Commonwealth of the Northern Mariana Islands. Severe habitat degradation has occurred on Sarigan because of overgrazing by introduced goats and pigs. Planting of coconut palms (Cocos nucifera) for copra production has also eliminated much of the island’s native forest. We recorded five species of forest birds on Sarigan: Micronesian Honeyeater (Myzomela rubratra), Micronesian Megapode (Megapodius laperouse laperouse), Micronesian Starling (Aplonis opaca), Collared Kingfisher (Halcyon chloris), and White-throated Ground Dove (Gallicolumba xanthonura). Estimated population sizes (95% confidence interval) in 1997 were 1,821 (1,617–2,026) for Micronesian Honeyeater, 677 (545–810) for Micronesian Megapode, 497 (319–675) for Micronesian Starling, 107 (82–131) for Collared Kingfisher, and 170 (101–238) for Mariana Fruit Bat (Pteropus mariannus).

  6. Chamorro Orthography: A Final Report on the Decisions of the Marianas Orthography Committee.

    ERIC Educational Resources Information Center

    Topping, Donald M.

    This paper presents a summary of the decisions made by the members of the Marianas Orthography Committee and contains recommendations and general rules for official and formal adoption into the writing system of the Chamorro language throughout the Mariana Islands. The committee presents the general rules with the hope that they will be followed…

  7. 77 FR 43416 - Application of Star Marianas Air, Inc. for Commuter Authority

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-24

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF TRANSPORTATION Office of the Secretary Application of Star Marianas Air, Inc. for Commuter Authority AGENCY: Department... tentatively finding Star Marianas Air, Inc., fit, willing, and able to provide scheduled passenger service...

  8. Microbial Community in the Hydrothermal System at Southern Mariana Trough

    NASA Astrophysics Data System (ADS)

    Kato, S.; Itahashi, S.; Kakegawa, T.; Utsumi, M.; Maruyama, A.; Ishibashi, J.; Marumo, K.; Urabe, T.; Yamagishi, A.

    2004-12-01

    There is unique ecosystem around deep-sea hydrothermal area. Living organisms are supported by chemical free energy provided by the hydrothermal water. The ecosystem is expected to be similar to those in early stage of life history on the earth, when photosynthetic organisms have not emerged. In this study, we have analyzed the microbial diversity in the hydrothermal area at southern Mariana trough. In the "Archaean Park Project" supported by special Coordination Fund, four holes were bored and cased by titanium pipes near hydrothermal vents in the southern Mariana trough in 2004. Hydrothermal fluids were collected from these cased holes and natural vents in this area. Microbial cells were collected by filtering the hydrothermal fluid in situ or in the mother sip. Filters were stored at -80C and used for DNA extraction. Chimneys at this area was also collected and stored at -80C. The filters and chimney samples were crushed and DNA was extracted. DNA samples were used for amplification of 16S rDNA fragments by PCR using archaea specific primers and universal primers. The PCR fragments were cloned and sequenced. These PCR clones of different samples will be compared. We will extend our knowledge about microbiological diversity at Southern Mariana trough to compare the results obtained at other area.

  9. Pollination of Picea orientalis (Pinaceae): saccus morphology governs pollen.

    PubMed

    Runions, C J; Rensing, K H; Takaso, T; Owens, J N

    1999-02-01

    Sacci of conifer pollen do not function primarily to increase the efficiency of wind pollination as is widely thought. Rather, they are bladders and cause pollen to float upwards in a liquid drop into the ovules. This observation is seemingly unsupported in the case of oriental spruce (Picea orientalis (L.) Link), which has saccate pollen. Ovulate cones are pendant at the time of pollination, which requires that pollen sink into the ovules. Pollen of oriental spruce floats at first but within 1-2 min sinks into the ovule. As sinking does not occur in saccate pollen of other Pinaceae, a variety of techniques was used to determine anatomical differences leading to this uncharacteristic tendency. Light, scanning electron, and confocal microscopy of the pollen surface yielded no significant appearing difference between pollen of oriental spruce and white spruce. However, transmission electron microscopy of freeze-fixed/freeze-substituted hydrated pollen revealed that the ektexine of oriental spruce pollen sacci is porous compared to that of white spruce. Confocal microscopy allowed examination of pollen hydration dynamics. Water enters pollen at the distal pole between sacci, and resulting rapid expansion of the tube cell forces air out of the saccate space. White spruce pollen remains buoyant because of enclosed air pockets in the saccus ektexine. Evolutionary change in pollen wall anatomy with resultant loss of saccus function is correlated with a change in ovulate strobilus orientation at pollination in oriental spruce. A suite of characters interact in the conifer pollination mechanism, and concerted change in these characters may lead to speciation.

  10. Embolism formation during freezing in the wood of Picea abies.

    PubMed

    Mayr, Stefan; Cochard, Hervé; Améglio, Thierry; Kikuta, Silvia B

    2007-01-01

    Freeze-thaw events can cause embolism in plant xylem. According to classical theory, gas bubbles are formed during freezing and expand during thawing. Conifers have proved to be very resistant to freeze-thaw induced embolism, because bubbles in tracheids are small and redissolve during thawing. In contrast, increasing embolism rates upon consecutive freeze-thaw events were observed that cannot be explained by the classical mechanism. In this study, embolism formation during freeze-thaw events was analyzed via ultrasonic and Cryo-scanning electron microscope techniques. Twigs of Picea abies L. Karst. were subjected to up to 120 freeze-thaw cycles during which ultrasonic acoustic emissions, xylem temperature, and diameter variations were registered. In addition, the extent and cross-sectional pattern of embolism were analyzed with staining experiments and Cryo-scanning electron microscope observations. Embolism increased with the number of freeze-thaw events in twigs previously dehydrated to a water potential of -2.8 MPa. In these twigs, acoustic emissions were registered, while saturated twigs showed low, and totally dehydrated twigs showed no, acoustic activity. Acoustic emissions were detected only during the freezing process. This means that embolism was formed during freezing, which is in contradiction to the classical theory of freeze-thaw induced embolism. The clustered pattern of embolized tracheids in cross sections indicates that air spread from a dysfunctional tracheid to adjacent functional ones. We hypothesize that the low water potential of the growing ice front led to a decrease of the potential in nearby tracheids. This may result in freezing-induced air seeding.

  11. Accumulation of semi-volatile organic compounds in moss (Sphagnum Species) and spruce needles (Picea Mariana): Whole-leaf absorption vs. surface adsorption processes

    SciTech Connect

    Hornbuckle, K.C.; Eisenreich, S.J.

    1996-12-31

    Vapor exchange of semi-volatile organic compounds (SOCs) with plant surfaces may control the fate of SOCS far from their original sources. For example, plants may act as sinks for released SOCs by adsorbing the compounds and burying them upon the plant`s death. Evidence for this hypothesis lies in the accumulation of SOCs in peat moss. Alternatively, plants may act as temporary {open_quote}resting points{close_quote} for long-range transport of the compounds from warmer regions to cooler regions. Studies that show higher SOC concentrations in plants collected from cooler parts of the globe are evidence for this hypothesis. Whether vapor-phase SOCs are taken up or released by plants is expected to depend on characteristics of the compound, the plant, and the local climate. Theoretically, it has been predicted that temperature and SOC hydrophobicity should dominate vapor exchange. The effect of these two factors on vapor-plant exchange has been examined through two concurrent and related studies. The first study concerns the vapor SOC dynamics in a semi-remote forested bog in northern Minnesota. The second study concerns the measured distribution between vapor and plant-associated SOCs in the same bog.

  12. Soil data from fire and permafrost-thaw chronosequences in upland Picea mariana stands near Hess Creek and Tok, interior Alaska

    USGS Publications Warehouse

    O'Donnell, Jonathan A.; Harden, Jennifer W.; Manies, Kristen L.; Jorgenson, M. Torre; Kanevskiy, Mikhail; Xu, Xiaomei

    2013-01-01

    Soils of the Northern Circumpolar Permafrost region harbor 1,672 petagrams (Pg) (1 Pg = 1,000,000,000 kilograms) of organic carbon (OC), nearly 50 percent of the global belowground OC pool (Tarnocai and others, 2009). Of that soil OC, nearly 88 percent is presently stored in perennially frozen ground. Recent climate warming at northern latitudes has resulted in warming and thawing of permafrost in many regions (Osterkamp, 2007), which might mobilize OC stocks from associated soil reservoirs via decomposition, leaching, or erosion. Warming also has increased the magnitude and severity of wildfires in the boreal region (Turetsky and others, 2011), which might exacerbate rates of permafrost degradation relative to warming alone. Given the size and vulnerability of the soil OC pool in permafrost soils, permafrost thaw will likely function as a strong positive feedback to the climate system (Koven and others, 2011; Schaefer and others, 2011). In this report, we report soil OC inventories from two upland fire chronosequences located near Hess Creek and Tok in Interior Alaska. We sampled organic and mineral soils in the top 2 meters (m) across a range of stand ages to evaluate the effects of wildfire and permafrost thaw on soil C dynamics. These data were used to parameterize a simple process-based fire-permafrost-carbon model, which is described in detail by O’Donnell and others (2011a, b). Model simulations examine long-term changes in soil OC storage in response to fire, permafrost thaw, and climate change. These data also have been used in other papers, including Harden and others (2012), which examines C recovery post-fire, and Johnson and others (2011), which synthesizes data within the Alaska Soil Carbon Database. Findings from these studies highlight the importance of climate and disturbance (wildfire, permafrost thaw) on soil C storage, and loss of soil C from high-latitude ecosystems.

  13. Seismic hazard assessment for Guam and the Northern Mariana Islands

    USGS Publications Warehouse

    Mueller, Charles S.; Haller, Kathleen M.; Luco, Nicholas; Petersen, Mark D.; Frankel, Arthur D.

    2012-01-01

    We present the results of a new probabilistic seismic hazard assessment for Guam and the Northern Mariana Islands. The Mariana island arc has formed in response to northwestward subduction of the Pacific plate beneath the Philippine Sea plate, and this process controls seismic activity in the region. Historical seismicity, the Mariana megathrust, and two crustal faults on Guam were modeled as seismic sources, and ground motions were estimated by using published relations for a firm-rock site condition. Maps of peak ground acceleration, 0.2-second spectral acceleration for 5 percent critical damping, and 1.0-second spectral acceleration for 5 percent critical damping were computed for exceedance probabilities of 2 percent and 10 percent in 50 years. For 2 percent probability of exceedance in 50 years, probabilistic peak ground acceleration is 0.94 gravitational acceleration at Guam and 0.57 gravitational acceleration at Saipan, 0.2-second spectral acceleration is 2.86 gravitational acceleration at Guam and 1.75 gravitational acceleration at Saipan, and 1.0-second spectral acceleration is 0.61 gravitational acceleration at Guam and 0.37 gravitational acceleration at Saipan. For 10 percent probability of exceedance in 50 years, probabilistic peak ground acceleration is 0.49 gravitational acceleration at Guam and 0.29 gravitational acceleration at Saipan, 0.2-second spectral acceleration is 1.43 gravitational acceleration at Guam and 0.83 gravitational acceleration at Saipan, and 1.0-second spectral acceleration is 0.30 gravitational acceleration at Guam and 0.18 gravitational acceleration at Saipan. The dominant hazard source at the islands is upper Benioff-zone seismicity (depth 40–160 kilometers). The large probabilistic ground motions reflect the strong concentrations of this activity below the arc, especially near Guam.

  14. Terrestrial bird population trends on Aguiguan (Goat Island), Mariana Islands

    USGS Publications Warehouse

    Amidon, Fred; Camp, Richard J.; Marshall, Ann P.; Pratt, Thane K.; Williams, Laura; Radley, Paul; Cruz, Justine B.

    2014-01-01

    The island of Aguiguan is part of the Mariana archipelago and currently supports populations of four endemic species, including one endemic genus, Cleptornis. Bird population trends since 1982 were recently assessed on the neighbouring islands of Saipan, Tinian, and Rota indicating declines in some native species. Point-transect surveys were conducted in 2008 by the U.S. Fish and Wildlife Service to assess population densities and trends on Aguiguan. Densities for six of the nine native birds—White-throated Ground-dove Gallicolumba xanthonura, Collared Kingfisher Todiramphus chloris, Rufous Fantail Rhipidura rufifrons, Golden White-eye Cleptornis marchei, Bridled White-eye Zosterops conspicillatus and Micronesian Starling Aplonis opaca—and the non-native bird—Island Collared-dove Streptopelia bitorquata—were significantly greater in 2008 than in 1982. No differences in densities were detected among the surveys for Mariana Fruit-dove Ptilinopus roseicapilla, and Micronesian MyzomelaMyzomela rubratra. Three federally and locally listed endangered birds—Nightingale Reed-warbler Acrocephalus luscinius, Mariana Swiftlet Collocalia bartschi, and Micronesian Megapode Megapodius laperous)—were either not detected during the point-transect counts, the surveys were not appropriate for the species, or the numbers of birds detected were too small to estimate densities. The factors behind the increasing trends for some species are unknown but may be related to increased forest cover on the island since 1982. With declining trends for some native species on neighbouring islands, the increasing and stable trends on Aguiguan is good news for forest bird populations in the region, as Aguiguan populations can help support conservation efforts on other islands in the archipelago.

  15. Hf isotope and concentration systematics of the Mariana arc

    NASA Astrophysics Data System (ADS)

    Tollstrup, D. L.; Gill, J. B.

    2004-12-01

    Negative Hf concentration anomalies are common but little-discussed geochemical features of island arcs. Because both light rare earth elements (LREE) and Hf may be mobile even in `fluid-dominated' island arcs, it is important to relate their isotopic and elemental ratios to models of slab-mantle mixing. We report new Hf isotope and trace element data for K-rich submarine basalts from the Kasuga seamounts located 10-20 km behind the volcanic front of the southern Northern Seamount Province (NSP) of the Mariana arc. These data, when combined with published data for other Mariana samples, span the full range from low-K tholeiites to high-K shoshonites. Rear-arc Kasuga seamounts seamounts of the NSP have lower 143Nd/144Nd and 176Hf/177Hf ratios than arc-front volcanoes of the Mariana Central Island Province (CIP). Within the CIP, Hf concentration anomalies correlate positively with 176Hf/177Hf ratios. Radiogenic Hf and little or no concentration anomalies characterize samples from fluid-dominated volcanoes (Guguan and Maug), whereas samples from sediment-melt dominated volcanoes (Anatahan and Sarigan) have less radiogenic Hf and larger concentration anomalies. Samples from the Kasuga and Hiyoshi seamounts have even larger negative concentration anomalies and less radiogenic Hf, although the two are not always correlated. These data are consistent with mixing between a depleted mantle and a partial melt of subducted sediment that is saturated with trace accessory phases including zircon, rutile, and monazite. A more volcaniclastic source is needed for the NSP than the CIP. Implications of these findings are three-fold. Partial melts of subducting sediment affect the HFSE and REE budgets of even fluid-dominated island arcs. Slab temperatures must be high enough for a peraluminous melt to be present, even where old, cold slabs are subducting. Refractory accessory phases have the potential to become exotic "nuggets" in the convecting mantle, potentially controlling the

  16. Seafloor magnetotelluric soundings in the Mariana Island Arc area

    NASA Astrophysics Data System (ADS)

    Filloux, J. H.

    Two seafloor magnetotelluric soundings have been performed in the Mariana Island arc and subduction area, the first (station 1) in the Mariana Trough near International Phase of Oceanic Drilling (IPOD) hole 454 (position 18° 01'N, 144° 32'E, depth 3770 m), the second (station 2) in the fore arc basin near IPOD hole 458 (position 18° 06'N, 146° 45'E, depth 3602 m). The electrical conductivity beneath the postulated spreading zone of the Mariana Trough appears to be unexpectedly low in the upper 40 km, increasing slowly and monotonically downward, to 1 S m-1 at 700 km. It does not display any significant feature such as lithosphere-asthenosphere or phase transition boundaries. The character of this profile differs considerably from those obtained near the Pacific Rise, suggesting deep as well as shallow structures, generally cooler, and implying less active magmatic processes. Cooler structures may in turn contribute in part to the greater depth versus age of the Mariana Trough compared to that of the main oceanic basins. No indication of the existence of extensive magma concentration of the kind detected on the Pacific Rise at 21°N is recognizable in the magnetic data. This fact, however, may simply result from the distance between station 1 and the spreading axis (˜30 km). A cautious speculation on the cause of the implied overall low conductivity values is presented. The MT sounding from the fore arc basin points to (1) very high conductance in the upper zone (0-10 km), accountable for by the sediment blanket, (2) moderate to high conductance in the fore arc upper mantle wedge below, possibly indicative of a moderately high temperature (composition and hydration by water subducted with sediments may also play a role, (3) a large cross section (300-400 km) of unusually little conducting materials, believed to represent the sinking slab and the cooled down environment adjacent to it, and (4) a 20-fold conductivity increase around 420 km depth, sustained over

  17. The crustaceans and pycnogonids of the Mariana Islands

    SciTech Connect

    Paulay, Gustav; Kropp, Roy K. ); Ng, Peter K.; Eldredge, Lucius G.

    2003-09-01

    The crustacean and pycnogonid fauna of the Mariana Islands is reviewed, and 829 crustacean and 15 pycnogonid species are documented from the archipelago based on literature records and new collections, including 272 new records. Voucher specimens are listed for 605 and photographic records for 356 species. The bulk of the fauna is marine, including 12 terrestrial and 11 freshwater decapods with marine larvae. Five cladocerans comprise the known freshwater fauna, and 25 peracarids and one copepod are currently documented on land. Coverage reflects a taxonomically uneven effort, and is strongly biased toward macrocrustaceans, with decapods accounting for 80%, and crabs for 50% of the recorded crustacean diversity.

  18. Emplacement and Growth of Serpentinite Seamounts on the Mariana Forearc

    NASA Astrophysics Data System (ADS)

    Oakley, A. J.; Taylor, B.; Moore, G. F.; Fryer, P.; Morgan, J. K.; Goodliffe, A. M.

    2004-12-01

    Seamounts comprised primarily of serpentinite muds are found on the outer forearc of the Izu-Bonin-Mariana subduction system. They represent some of the first material outputs of the recycling process that takes place in subduction zones. Therefore, understanding their evolution is necessary to correctly quantify the flux of material through the subduction system. Serpentinite seamounts have been described as mud diapirs, mud volcanoes, uplifted blocks of mantle material, and a composite of the latter two. Multi-channel seismic (MCS) data collected in 2002 from the outer Mariana forearc imaged, for the first time, the large-scale internal structure of these seamounts. These data, combined with new bathymetry, have provided insight into how the seamounts grow and deform with time and have allowed us to evaluate proposed models for their formation. The serpentinite seamounts rest on faulted and sedimented Mariana forearc basement. Flank flows of serpentinite muds downlap existing forearc substrate, leaving the underlying stratigraphy largely undisturbed. Reflections located 3.5-5 km beneath forearc basement may represent Moho, suggesting that the seamounts are built on anomalously thin forearc crust. A strong reflection at the summit of Big Blue, the largest serpentinite seamount in the Mariana Forearc, represents a collapse structure that has been partially in-filled by younger muds, supporting the idea that serpentinite seamount growth is episodic. Basal thrusts that incorporate forearc sediments at the toe of Turquoise Seamount provide evidence for seamount settling and lateral growth. We are conducting numerical simulations of seamount growth and evolution using the discrete element method (DEM), previously used to examine gravity spreading phenomena in magmatic volcanoes. Simulations employing distinctly low basal and internal friction coefficients provide a good match to the overall morphology of the serpentinite seamounts, and offer insight into their internal

  19. Warming-Induced Decline of Picea crassifolia Growth in the Qilian Mountains in Recent Decades.

    PubMed

    Yu, Li; Huang, Lei; Shao, Xuemei; Xiao, Fengjing; Wilmking, Martin; Zhang, Yongxiang

    2015-01-01

    Warming-induced drought has widely affected forest dynamics in most places of the northern hemisphere. In this study, we assessed how climate warming has affected Picea crassifolia (Qinghai spruce) forests using tree growth-climate relationships and the normalized difference vegetation index (NDVI) along the Qilian Mountains, northeastern Tibet Plateau (the main range of Picea crassifolia). Based on the analysis on trees radial growth data from the upper tree line and the regional NDVI data, we identified a pervasive growth decline in recent decades, most likely caused by warming-induced droughts. The drought stress on Picea crassifolia radial growth were expanding from northeast to southwest and the favorable moisture conditions for tree growth were retreating along the identical direction in the study area over the last half century. Compared to the historical drought stress on tree radial growth in the 1920s, recent warming-induced droughts display a longer-lasting stress with a broader spatial distribution on regional forest growth. If the recent warming continues without the effective moisture increasing, then a notable challenge is developed for Picea crassifolia in the Qilian Mountains. Elaborate forest management is necessary to counteract the future risk of climate change effects in this region.

  20. Geology of Saipan, Mariana Islands; Part 1, General geology

    USGS Publications Warehouse

    Cloud, Preston E.; Schmidt, Robert George; Burke, Harold W.

    1956-01-01

    Saipan, situated about 15° N. and 146° E., is one of the larger and more southerly of the Mariana Islands. The 15 small islands of this chain are strung along an eastwardly convex ridge for more than 400 miles north to south, midway between Honshu and New Guinea and about 1,200 miles east of the Philippines. Paralleling this ridge 60 to 100 miles further east is a deep submarine trench, beyond which lies the Pacific Basin proper. To the west is the Philippine Sea, generally deeper than 2,000 fathoms. The trench coincides with a zone of negative gravity anomalies, earthquake foci occur at increasing depths westward from it, and silica- and alumina-rich volcanic rocks characterize the emergent island chain itself. The contrast between these features and those of the Pacific Basin proper to the east is held to favor the conclusion that the Mariana island arc and trench define the structural and petrographic front of Asia

  1. Explorations of Mariana Arc Volcanoes Reveal New Hydrothermal Systems

    NASA Astrophysics Data System (ADS)

    Embley, R. W.; Baker, E. T.; Chadwick, W. W., Jr.; Lupton, J. E.; Resing, J. A.; Massoth, G. J.; Nakamura, K.

    2004-01-01

    Some 20,000 km of volcanic arcs, roughly one-third the length of the global mid-ocean ridge (MOR) system, rim the western Pacific Ocean. Compared to 25 years of hydrothermal investigations along MORs, exploration of similar activity on the estimated ~600 submarine arc volcanoes is only beginning [Ishibashi and Urabe, 1995; De Ronde et al., 2003]. To help alleviate this under-sampling, the R/V T. G. Thompson was used in early 2003 (9 February to 5 March) to conduct the first complete survey of hydrothermal activity along 1200 km of the Mariana intra-oceanic volcanic arc. This region includes both the Territory of Guam and the Commonwealth of the Northern Mariana Islands. The expedition mapped over 50 submarine volcanoes with stunning new clarity (Figures 1 and 2) and found active hydrothermal discharge at 12 sites, including the southern back-arc site. This includes eight new sites along the arc (West Rota, Northwest Rota, E. Diamante, Zealandia Bank, Maug Caldera, Ahyi, Daikoku, and Northwest Eifuku) and four sites of previously known hydrothermal activity (Seamount X, Esmeralda, Kasuga 2, and Nikko) (Figures 1 and 2). The mapping also fortuitously provided a ``before'' image of the submarine flanks of Anatahan Island, which had its first historical eruption on 10 May 2003 (Figures 1 and 3).

  2. 75 FR 44231 - Defense Representative Guam, Commonwealth of the Northern Mariana Islands, Federated States of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-28

    ... the MIRC that are necessary if the Military Services are to maintain a state of military readiness... military training and Research, Development, Test, and Evaluation (RDT&E) activities in the Mariana...

  3. The Anatahan Felsic Province in the Mariana Arc System

    NASA Astrophysics Data System (ADS)

    Stern, R. J.; Hargrove, U. S.

    2003-12-01

    The May 2003 plinian eruption of Anatahan was a surprise for the residents of the Northern Mariana Mariana Comonwealth (USA). From a petrologic perspective, this eruption of siliceous andesite was also atypical for the Mariana arc, which elsewhere mostly erupts fractionated basalts and basaltic andesites. However, felsic eruptions are not unusual for Anatahan which has previously erupted a wide range of lavas, from basalts through dacites. The reasons and significance of the intermittently felsic nature of Anatahan lavas may reflect either development of a mature magma chamber localized beneath Anatahan or perhaps the presence of an areally-extensive pool of felsic magma in the middle crust, similar to that inferred for the Izu arc to the north. Anatahan lies at the southern end of the Mariana Central Island Province and just north of the Southern Seamount Province, but it has never been clear whether these morphological subdivisions reflect different magma fluxes along the arc or are due to the island volcanoes being older than the seamounts. Synthesis of existing data for Anatahan and data for surrounding seamounts collected during the 2001 Cook 7 expedition and earlier cruises indicates that Anatahan is situated near the middle of an arc segment with an unusually high proportion of felsic lavas compared to typical Mariana Arc magmatic products. The 115 km-long arc segment from E. Diamante seamount East Diamante seamount (15° 55'N) to a seamount NW of Zealandia Bank (17° N) define the Anatahan Felsic Province (AFP). Seven edifices within this region, including Anatahan, have erupted felsic lavas, often interspersed with more mafic lavas. Volcano size does not seem important in controlling the abundance of felsic lavas within the AFP; the volcanic island of Sarigan does not contain felsic lavas, whereas small parasitic cones NE of Anatahan and SW of E. Diamante erupt lavas with 65% and 72% SiO2, respectively. This region also contains two suspected hydrothermal

  4. Military Geology of Saipan, Mariana Islands. Volume 1: Introduction and Engineering Aspects

    DTIC Science & Technology

    1955-01-01

    Engineering geology 66 Engineering aspects of soils 67 MAPS Plate 1 Topographic map of Saipan, Mariana Islands, and regional relationships in...pocket 5 Engineering soils map of Saipan in pocket PHOTOGRAPHS Following page Plate 6 A. Very compact limestone, Laderan Dago 22 B. Very compact...to north, the main islands of the Marianas are Guam, Rota, Aguijan, Tinian, Saipan, Farallon de Medlnilla, Anatahan, Sarigan, Guguan (Gugan

  5. The Southern Mariana Forearc: An Active Subduction Initiation (SI) Analogue

    NASA Astrophysics Data System (ADS)

    Stern, R. J.; Bloomer, S. H.; Brounce, M. N.; Ishii, T.; Ishizuka, O.; Kelley, K. A.; Martinez, F.; Ohara, Y.; Pujana, I.; Reagan, M. K.; Ribeiro, J.

    2014-12-01

    It is important to understand how new subduction zones form. Some subduction zones begin spontaneously, with sinking of dense oceanic lithosphere adjacent to a lithospheric weakness. The Eocene evolution of the Izu-Bonin-Mariana convergent margin is the type example of this process, with an increasingly well-documented evolution including results from IODP 352 drilling. A lack of any active examples of spontaneous SI hinders our understanding, but our studies of the evolution of the southernmost Mariana convergent margin provides important insights. Here the Mariana Trough backarc basin terminates against the Challenger Deep trench segment, where it has opened ~250 km in the past ~4 Ma. This corresponds to GPS opening rate of ~4.5cm/y at the latitude of Guam (Kato et al., 2003). This newly formed and rapidy widening margin faces the NW-converging Pacific plate and causes it to contort and tear. Pacific plate continues to move NW but the upper plate response is illustrative of a newly formed subduction zone. Slab-related earthquakes can be identified to ~200 km deep beneath this margin; with convergence rate of 3cm/yr, this may reflect no more than 7 Ma of subduction. The usual well-defined magmatic arc is missing; its position ~100 km above the subducted slab is occupied by the magma-rich (inflated) Malaguana-Gadao Ridge (MGR), and hydrous MORB-like basalts with ~2 wt. % H2O have erupted unusually close to the trench where they overly mantle peridotites ~6 km water depth. HMR-1 sonar backscatter mapping reveals a chaotic fabric that is at a high angle to the trend of the MGR to the east but is concordant to the west. This unusual spreading fabric may have formed by chaotic upper plate extension in response to rapid rollback of the short, narrow Pacific slab in a manner similar to that thought to occur during SI. Further interdisciplinary studies are needed to understand this rapidly-evolving tectono-magmatic province and what it can teach us about SI.

  6. High-Throughput Sequencing Reveals Drastic Changes in Fungal Communities in the Phyllosphere of Norway Spruce (Picea abies) Following Invasion of the Spruce Bud Scale (Physokermes piceae).

    PubMed

    Menkis, Audrius; Marčiulynas, Adas; Gedminas, Artūras; Lynikienė, Jūratė; Povilaitienė, Aistė

    2015-11-01

    The aim of this study was to assess the diversity and composition of fungal communities in damaged and undamaged shoots of Norway spruce (Picea abies) following recent invasion of the spruce bud scale (Physokermes piceae) in Lithuania. Sampling was done in July 2013 and included 50 random lateral shoots from ten random trees in each of five visually undamaged and five damaged 40-50-year-old pure stands of P. abies. DNA was isolated from 500 individual shoots, subjected to amplification of the internal transcribed spacer of fungal ribosomal DNA (ITS rDNA), barcoded and sequenced. Clustering of 149,426 high-quality sequences resulted in 1193 non-singleton contigs of which 1039 (87.1 %) were fungal. In total, there were 893 fungal taxa in damaged shoots and 608 taxa in undamaged shoots (p < 0.0001). Furthermore, 431 (41.5 %) fungal taxa were exclusively in damaged shoots, 146 (14.0 %) were exclusively in undamaged shoots, and 462 (44.5 %) were common to both types of samples. Correspondence analysis showed that study sites representing damaged and undamaged shoots were separated from each other, indicating that in these fungal communities, these were largely different and, therefore, heavily affected by P. piceae. In conclusion, the results demonstrated that invasive alien tree pests may have a profound effect on fungal mycobiota associated with the phyllosphere of P. abies, and therefore, in addition to their direct negative effect owing physical damage of the tissue, they may also indirectly determine health, sustainability and, ultimately, distribution of the forest tree species.

  7. Biology of Scolytus piceae (Coleoptera: Curculionidae: Scolytinae) in Northern Idaho and Notes on Comparative Anatomical Features of the Larva.

    PubMed

    Furniss, Malcolm M; Kegley, Sandra J

    2017-03-20

    The forests of the northern Rocky Mountains are hosts of nine species of Nearctic conifer-infesting bark beetles of the genus Scolytus Geoffroy. One of these, Scolytus piceae Swaine (Coleoptera: Curculionidae), infests spruces across the continent including Picea engelmannii Parry (Pinaceae) in Idaho. Mature larvae overwintered before transforming to adults, which infested new hosts in early June. Scolytus piceae most commonly infested shaded out lower branches of mature live spruce. In sporadic wind-felled spruce, the beetle infested the terminal portion of branches throughout the crown, whereas another Scolytinae, Polygraphus rufipennis (Kirby), infested the thicker bark basal portion of the same branches. This niche separation resulted from difference in time of their dispersal flight and ability of S. piceae to colonize thin bark by engraving the underlying sapwood. Population recruitment of S. piceae was low in shaded out lower branches, in balance with this limited host resource. Features of the head of S. piceae larvae differed from that reported in literature, including the mandible dentition, antennal field, and pattern of setae on the postlabium.

  8. [Pathways and rates of Pinus sylvestris L. and Picea species recolonization into Scandinavia in Holocene].

    PubMed

    Sannikov, S N; Sannikova, N S

    2015-01-01

    The results are presented of comparative analysis of pathways, rates, and timing of recolonization into Scandinavia, in Holocene, of Pinus sylvestris populations and those of Picea abies and P. obovata. The dispersion rate, starting from 12 thou years before present (BP), is calculated using palynological data from scientific literature on radiometric dating. It is found out that P sylvestris spread into Central Scandinavia from the Alps via the Danish Isthmus about 8.2 thou years BP with the speed of 500-1250 km per 1 thou years. A hypothesis is put forward suggesting that such a fast speed is due to pine seeds hydrochory, which is much faster than anemochory according to our researches. From the northern part of the East European Plain, P. sylvestris spread into Fennoscandia with lower speed (520 km per 1 thou years). Populations of Picea species dispersed from the same regions with speed (131-164 km per 1 thou years) 3-10 times lower than that of P. sylvestris. Therefore, invasion of Picea abies from the Alps into Scandinavia via the Danish Isthmus did not have time to happen before the formation of the Kattegat Strait. By circumferential pathway, through Karelia, both species of Picea reached the northern parts of Scandinavia only 3.5 thou years BP, its central parts - 2 thou years BP, and its southern parts - 1.5 thou years BP, i.e., later than P. sylvestris by 4, 6.2, and 8.5 thou years respectively. Probably, this may be explained by the fact that in pines the time to seeding is twofold shorter, while their sprouts were more tolerant to climatic extremums in periglacial habitats in middle Holocene.

  9. Analysis of the Phialocephala subalpina Transcriptome during Colonization of Its Host Plant Picea abies

    PubMed Central

    Reininger, Vanessa; Schlegel, Markus

    2016-01-01

    Background Phialocephala subalpina belongs to the Phialocephala fortinii s.l.–Acepphala applanata species complex (PAC) forming one of the major groups belonging to the dark septate endophytes (DSE). Depending on the strain, PAC was shown to form neutral to pathogenic associations with its host plant Picea abies. To understand PACs lifestyle we investigated the effect of presence/absence of Picea abies on the transcriptome of strain 6_70_1. Materials and Methods PAC strain 6_70_1 was grown in liquid Pachlewski media either induced by its host plant Picea abies or without host plant as a control. Mycelia were harvested in a time course (1, 2, 3, 4, 7, 11, 18 days) with and without induction by the host plant and the fungal transcriptome revealed by Illumina sequencing. Differential gene expression analysis over the time course comparing control and treatment at each time point using the ‘edgeR glm approach’ and a gene enrichment analysis using GO categories were performed. Results The three main functional groups within differentially expressed genes were ‘metabolism’, ‘transport’ and ‘cell rescue, defense and virulence’. Additionally, genes especially involved in iron metabolism could be detected by gene set enrichment analysis. Conclusion In conclusion, we found PAC strain 6_70_1 to be metabolically very active during colonization of its host plant Picea abies. A major shift in functional groups over the time course of this experiment could not be observed but GO categories which were found to be enriched showed different emphasis depending in the day post induction. PMID:26954682

  10. [Genetic control of isozymes in European spruces (Picea abies (L) Karst) of the Ukrainian Carpathian mountains].

    PubMed

    Privalikhin, S N; Korshikov, I I; Pirko, N N; Velikorid'ko, T I; Pirko, Ia V

    2006-01-01

    Genetical control of the enzymes GOT, GDH, DIA, MDH, SOD, FDH, ADH, ACP and LAP has been studied in nine natural Carpathian populations of Norway spruce (Picea abies (L.) Karst.) using polyacrylamide gel elecrophoresis and analysis of isozyme variability in 346 trees. Seventy one allel products of 20 gene loci have been clearly established. Segregation analysis of the revealed allele variants confirms their monogenic inheritance.

  11. Rapid detection of Ophiostoma piceae and O. quercus in stained wood by PCR.

    PubMed

    Kim, S H; Uzunovic, A; Breuil, C

    1999-01-01

    A rapid, sensitive, and simple method was developed to detect the sapstain fungi Ophiostoma piceae and O. quercus in stained wood. By using microwave heating for DNA extraction and PCR with internal transcribed spacer-derived-specific primers, detection was feasible within 4 h, even with DNA obtained from a single synnema. This method can easily be extended for the detection of other wood-inhabiting fungi.

  12. Conservation and divergence of gene expression plasticity following c. 140 million years of evolution in lodgepole pine (Pinus contorta) and interior spruce (Picea glauca×Picea engelmannii).

    PubMed

    Yeaman, Sam; Hodgins, Kathryn A; Suren, Haktan; Nurkowski, Kristin A; Rieseberg, Loren H; Holliday, Jason A; Aitken, Sally N

    2014-07-01

    Species respond to environmental stress through a combination of genetic adaptation and phenotypic plasticity, both of which may be important for survival in the face of climatic change. By characterizing the molecular basis of plastic responses and comparing patterns among species, it is possible to identify how such traits evolve. Here, we used de novo transcriptome assembly and RNAseq to explore how patterns of gene expression differ in response to temperature, moisture, and light regime treatments in lodgepole pine (Pinus contorta) and interior spruce (a natural hybrid population of Picea glauca and Picea engelmannii). We found wide evidence for an effect of treatment on expression within each species, with 6413 and 11,658 differentially expressed genes identified in spruce and pine, respectively. Comparing patterns of expression among these species, we found that 74% of all orthologs with differential expression had a pattern that was conserved in both species, despite 140 million yr of evolution. We also found that the specific treatments driving expression patterns differed between genes with conserved versus diverged patterns of expression. We conclude that natural selection has probably played a role in shaping plastic responses to environment in these species.

  13. Modular organization of the white spruce (Picea glauca) transcriptome reveals functional organization and evolutionary signatures.

    PubMed

    Raherison, Elie S M; Giguère, Isabelle; Caron, Sébastien; Lamara, Mebarek; MacKay, John J

    2015-07-01

    Transcript profiling has shown the molecular bases of several biological processes in plants but few studies have developed an understanding of overall transcriptome variation. We investigated transcriptome structure in white spruce (Picea glauca), aiming to delineate its modular organization and associated functional and evolutionary attributes. Microarray analyses were used to: identify and functionally characterize groups of co-expressed genes; investigate expressional and functional diversity of vascular tissue preferential genes which were conserved among Picea species, and identify expression networks underlying wood formation. We classified 22 857 genes as variable (79%; 22 coexpression groups) or invariant (21%) by profiling across several vegetative tissues. Modular organization and complex transcriptome restructuring among vascular tissue preferential genes was revealed by their assignment to coexpression groups with partially overlapping profiles and partially distinct functions. Integrated analyses of tissue-based and temporally variable profiles identified secondary xylem gene networks, showed their remodelling over a growing season and identified PgNAC-7 (no apical meristerm (NAM), Arabidopsis transcription activation factor (ATAF) and cup-shaped cotyledon (CUC) transcription factor 007 in Picea glauca) as a major hub gene specific to earlywood formation. Reference profiling identified comprehensive, statistically robust coexpressed groups, revealing that modular organization underpins the evolutionary conservation of the transcriptome structure.

  14. Genomic and phenotypic architecture of a spruce hybrid zone (Picea sitchensis × P. glauca).

    PubMed

    Hamilton, Jill A; Lexer, Christian; Aitken, Sally N

    2013-02-01

    Interspecific hybridization may enhance the capacity of populations to adapt to changing environments, and has practical implications for reforestation. We use genome-wide estimates of admixture and phenotypic traits for trees in a common garden to examine the extent and direction of gene flow across a Picea hybrid zone, testing assumptions of the bounded hybrid superiority and tension zone models of hybrid zone maintenance. Seeds were collected from the ecological transition zone spanning from maritime to continental climates across the Picea sitchensis-P. glauca contact zone, and 721 trees were planted in a common garden experiment within the hybrid zone. Individuals were genotyped using a panel of 384 candidate-gene single nucleotide polymorphisms (SNPs) putatively associated with adaptive traits in Picea, and phenotyped at age ten for height and autumn cold hardiness. Low interspecific heterozygosity in hybrids indicated that intrinsic reproductive barriers were too weak to prevent widespread recombination, although introgression appeared asymmetric with P. sitchensis dominating the zone. Whereas marker-based hybrid index was strongly correlated with climate and geography, phenotypic traits exhibited weak or no significant clines. Our results indicated that exogenous selection appeared to play a strong role in the distribution and structure of this hybrid zone, indicative of an environmentally determined bounded hybrid superiority model of hybrid zone maintenance, although endogenous mechanisms could not be ruled out. This study provides insight into the mechanisms underlying adaptation across ecologically transitional hybrid zones that will ultimately provide an additional tool in managing these economically important tree species.

  15. 9 CFR 72.3 - Areas quarantined in the Virgin Islands of the United States, the Northern Mariana Islands, the...

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... Islands of the United States, the Northern Mariana Islands, the Commonwealth of Puerto Rico, and the Island of Guam. 72.3 Section 72.3 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE... BABESIOSIS § 72.3 Areas quarantined in the Virgin Islands of the United States, the Northern Mariana...

  16. 33 CFR 334.1420 - Pacific Ocean off Orote Point, Apra Harbor, Island of Guam, Marianas Islands; small arms firing...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ..., Apra Harbor, Island of Guam, Marianas Islands; small arms firing range. 334.1420 Section 334.1420... AND RESTRICTED AREA REGULATIONS § 334.1420 Pacific Ocean off Orote Point, Apra Harbor, Island of Guam, Marianas Islands; small arms firing range. (a) The danger zone. The waters within an area delineated by...

  17. 78 FR 7385 - Western Pacific Fisheries; Fishing in the Marianas Trench, Pacific Remote Islands, and Rose Atoll...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-01

    ...; Fishing in the Marianas Trench, Pacific Remote Islands, and Rose Atoll Marine National Monuments AGENCY... Marianas Trench, Pacific Remote Islands, and Rose Atoll Marine National Monuments. DATES: NMFS must receive..., ``Establishment of the Rose Atoll Marine National Monument'' (74 FR 1577, January 12, 2009). The...

  18. 78 FR 12015 - Western Pacific Fisheries; Fishing in the Marianas Trench, Pacific Remote Islands, and Rose Atoll...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-21

    ...; Fishing in the Marianas Trench, Pacific Remote Islands, and Rose Atoll Marine National Monuments AGENCY... fishing in the Marianas Trench, Pacific Remote Islands, and Rose Atoll Marine National Monuments. The...). Proclamation 8337 of January 6, 2009, ``Establishment of the Rose Atoll Marine National Monument'' (74 FR...

  19. 3 CFR 8335 - Proclamation 8335 of January 6, 2009. Establishment of the Marianas Trench Marine National Monument

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Pacific Plate plunges beneath the Philippine Sea Plate and into the Earth's mantle, creating the Mariana... Farallon de Pajaros (also known as Uracas), Maug, and Asuncion support some of the largest biomass of reef... Farallon de Pajaros, Maug, and Asuncion in the Commonwealth of the Northern Mariana Islands, and...

  20. Marianas Test of English Achievement: Listening Comprehension, Structure and Vocabulary, Reading Comprehension. Manual, 1979 Edition, Forms A and B.

    ERIC Educational Resources Information Center

    Baldauf, Richard B., Jr.; Klingbergs, Imants E.

    This manual for the Marianas Test of English Achievement (MTEA) provides a description of the tests, directions for administration and scoring, a discussion of the interpretation of scores, and norms based on all 1,780 children in grades 6 through 9 in the Northern Marianas Islands in October 1977 and May 1978. There are two equivalent forms of…

  1. Population characteristics, habitat and diet of a recently discovered stingray Dasyatis marianae: implications for conservation.

    PubMed

    Costa, T L A; Thayer, J A; Mendes, L F

    2015-01-06

    This study examined population density, habitat and diet of Dasyatis marianae, a recently discovered species, in the reef complex of Maracajaú in Rio Grande do Norte state, Brazil. The highest concentration of D. marianae occurred in seagrass beds. Habitat use differed significantly between sex and age classes, with females and juveniles using areas other than reefs. Females utilized primarily seagrass beds and juveniles occurred mainly along the sandy bottom near the beach, highlighting the importance of protecting these areas. Dasyatis marianae diet was characterized primarily by crustaceans (91·9% index of relative importance, IRI ), including shrimp, crabs and lobsters. The availability of prey in different habitat types influences occupation by D. marianae, but the prey selectivity of D. marianae, among other factors, may affect this relationship. Intense shrimp and lobster fishing in the region probably has an effect on preferred prey resources of this ray. Information on feeding habits of this species contributes to a better understanding of trophic dynamics and food webs, which is critical if ecosystem principles are to be integrated into fisheries management.

  2. Geology and Petrology of the Southeast Mariana Forearc Rift

    NASA Astrophysics Data System (ADS)

    Ribeiro, J. M.; Anthony, E. Y.; Bloomer, S. H.; Girard, G.; Ishizuka, O.; Kelley, K. A.; Manton, W. I.; Martinez, F.; Merle, S. G.; Ohara, Y.; Reagan, M. K.; Ren, M.; Stern, R. J.

    2011-12-01

    The southernmost Mariana convergent margin is tectonically and magmatically very active, with submarine arc volcanoes that are sub-parallel to the Malaguana-Gadao Ridge backarc spreading center at ~110km from the trench axis. This activity reflects widening of the S. Mariana Trough. Stretching formed 3 southeast-facing, broad rifts extending from the trench to an extinct arc volcano chain (~80km from the trench axis) that is mostly composed of outcrops and fragments of pillow lavas partially covered by sediments. The 3 rifts comprise the S.E. Mariana Forearc Rift (SEMFR) and are 50-56km long and 3600 to 8200m deep, with axial valleys that narrow near the extinct arc. We studied the SEMFR using one Shinkai 6500 dive in 2008 and two Shinkai 6500 dives and 7 deep-tows in 2010. Near the trench, the SEMFR flanks are very steep and dominated by talus slopes of lava, fine-grained gabbro, diabase and peridotite, sometimes covered by thin volcaniclastic sediments. Few outcrops of pillow lavas, lava flows and volcaniclastics are observed, strongly suggesting that SEMFR morphology is dominated by faulting and landsliding. Lava outcrops are smoother and better preserved towards the extinct arc, suggesting that magmatic activity dominates that part of the rift. 40Ar-39Ar ages of 3 SEMFR lavas are 3.0-3.7Ma, so post-magmatic rifting is younger than ~3Ma. SEMFR pillow lavas are vesicular and microporphyritic with crystallite-rich glassy rinds, indicating they erupted underwater at near-liquidus conditions. In contrast, the lava flows are more crystallized and less vesicular. SEMFR lavas exhibit similar ranges in mineral composition with 2 kinds of plagioclase (An>80% and An<80%), clinopyroxene (Mg#≥80% and Mg#<80%), olivine (Fo>90 and Fo<90), suggesting magma mixing. Gabbroic rocks are slightly altered and have olivine and clinopyroxene compositions similar to those of the lavas, but contain less anorthitic plagioclase with a wider range in composition (An20-70) than the lavas

  3. Identifying ecological barriers in marine environment: The case study of Dasyatis marianae.

    PubMed

    Costa, Tiego L A; Pennino, M Grazia; Mendes, Liana F

    2017-04-01

    Ecological barriers are important determinants of the evolution and distributions marine organisms, and a challenge for evolutionary ecologists seeking to understand population structure in the sea. Dasyatis marianae is an endemic Brazilian species that indicates certain restrictions on its distribution probably due to marine barriers. In this study, Bayesian hierarchical spatial models, jointly with environmental and occurrence species data, are used to identify, which elements could generate these barriers on Dasyatis marianae distribution. Results show that salinity and temperature are the most important drivers that play an essential role to limit the distribution of this species. Indeed, low salinity values restrict Dasyatis marianae distribution in the north of the Brazilian coast, while in the south are colder temperatures. These results highlight the need to better define the distribution of marine species, especially for the ones affected by ecological barriers that are more sensitive to environmental changes.

  4. Metamorphic Devolatilization in the Izu-Bonin-Mariana Subduction Factory

    NASA Astrophysics Data System (ADS)

    Kerrick, D.; Connolly, J.

    2002-12-01

    Metamorphic devolatilization in subduction zones is essential to arc magmatism, seismicity and volatile recycling. Our premise is that realistic modeling of metamorphic devolatilization of subducted lithologies is only possible for chemical systems that closely approximate actual bulk compositions. Volatile components are introduced into subduction zones by three contrasting lithologies: marine sediments, and hydrothermally altered mantle ultramafic rocks (serpentinites) and oceanic metabasalts. Using free energy minimization (Perplex programs: erdw.ethz.ch/~jamie/perplex), phase equilibria were computed to 6 GPa (~160 km) to quantify the evolution of CO2 and H2O by prograde metamorphism of marine sediments and oceanic metabasalts entering the Izu-Bonin-Mariana (IBM) subduction system. Major oxide compositions for these protoliths (from sites 801 and 1149 ODP cores) were utilized as input for our computations. Assuming that the IBM system is a relatively cool subduction zone, we quantified devolatilization for the P-T path along the top of the subducted slab. Major dehydration of pelagic clays in sites 801 and 1149, and volcaniclastic turbidites in site 801, are predicted to occur in the subarc. The subjacent hyrothermally altered basalts in sites 801 and 1149 will undergo little devolatilization for cool geotherms. With the unsubstantiated assumption that serpentinites exist in the upper mantle of the subducted slab, this lithology would also provide a significant subarc H2O source. With serpentinite included in the subducted slab, and assuming a fluid-assisted embrittlement mechanism for earthquakes, metamorphic dehydration could account for the distribution of hypocenters along the top of the subucted slab of the IBM system. Serpentinite as a major H2O source for the Isu arc is compatible with the strong depletion in trace element concentrations of the arc volcanics [1]. Trace element signatures in the Mariana arc magmas [2] are consistent with subducted

  5. The extent and meaning of hybridization and introgression between Siberian spruce (Picea obovata) and Norway spruce (Picea abies): cryptic refugia as stepping stones to the west?

    PubMed

    Tsuda, Yoshiaki; Chen, Jun; Stocks, Michael; Källman, Thomas; Sønstebø, Jørn Henrik; Parducci, Laura; Semerikov, Vladimir; Sperisen, Christoph; Politov, Dmitry; Ronkainen, Tiina; Väliranta, Minna; Vendramin, Giovanni Giuseppe; Tollefsrud, Mari Mette; Lascoux, Martin

    2016-06-01

    Boreal species were repeatedly exposed to ice ages and went through cycles of contraction and expansion while sister species alternated periods of contact and isolation. The resulting genetic structure is consequently complex, and demographic inferences are intrinsically challenging. The range of Norway spruce (Picea abies) and Siberian spruce (Picea obovata) covers most of northern Eurasia; yet their geographical limits and histories remain poorly understood. To delineate the hybrid zone between the two species and reconstruct their joint demographic history, we analysed variation at nuclear SSR and mitochondrial DNA in 102 and 88 populations, respectively. The dynamics of the hybrid zone was analysed with approximate Bayesian computation (ABC) followed by posterior predictive structure plot reconstruction and the presence of barriers across the range tested with estimated effective migration surfaces. To estimate the divergence time between the two species, nuclear sequences from two well-separated populations of each species were analysed with ABC. Two main barriers divide the range of the two species: one corresponds to the hybrid zone between them, and the other separates the southern and northern domains of Norway spruce. The hybrid zone is centred on the Urals, but the genetic impact of Siberian spruce extends further west. The joint distribution of mitochondrial and nuclear variation indicates an introgression of mitochondrial DNA from Norway spruce into Siberian spruce. Overall, our data reveal a demographic history where the two species interacted frequently and where migrants originating from the Urals and the West Siberian Plain recolonized northern Russia and Scandinavia using scattered refugial populations of Norway spruce as stepping stones towards the west.

  6. The first Shinkai dive study of the southwestern Mariana arc system

    NASA Astrophysics Data System (ADS)

    Ohara, Y.; Martinez, F.; Brounce, M. N.; Pujana, I.; Ishii, T.; Stern, R. J.; Ribeiro, J.; Michibayashi, K.; Kelley, K. A.; Reagan, M. K.; Watanabe, H.; Okumura, T.; Oya, S.; Mizuno, T.

    2014-12-01

    The 3000 km long Izu-Bonin-Mariana (IBM) arc system is an outstanding example of an intraoceanic convergent plate margin. The IBM forearc is a typical nonaccretionary convergent plate margin; the inner trench slope exposes lithologies found in many ophiolites. To more clearly delineate the geology of the forearc, we have been investigating a ~500 km long region of the Mariana forearc south of ~13°N using the DSV Shinkai 6500 and deep-tow camera since 2006. Discoveries includes the presence of MORB-like basalts that formed during subduction initiation (~51 Ma) [Reagan et al., 2010, G3], a region of forearc rifting unusually close to the trench axis, the Southeast Mariana Forearc Rift [Ribeiro et al., 2013, G3], and a serpentinite-hosted ecosystem near the Challenger Deep, the Shinkai Seep Field [Ohara et al., 2012, PNAS]. However, there have been no studies on the southern Mariana area west of the Challenger Deep except one [Hawkins and Batiza, 1977, EPSL], hindering our understanding of the IBM system. To advance our biogeoscientific understanding of this region, a Shinkai 6500 diving cruise (YK14-13) was conducted in July 2014 on two major sites: the inner trench slope west of the Challenger Deep (Site A), and the southwesternmost tip of the Mariana Trough (Site B). Dives at Site A recovered very fresh mantle peridotite associated with troctolite and limestone. The limestone preserves the remnants of corals, clearly indicating that the limestone is an accreted material originating from the incoming (colliding) Caroline Ridge. The freshness of the peridotites also indicates that the collision is an ongoing event, resulting in a protruding peridotite ridge along the inner trench slope west of the Challenger Deep. Dives at Site B recovered basalt and gabbro, which is either new backarc basin crust or rifted West Mariana Ridge crust. This cruise allowed for continued sampling of the inner trench slope of the Mariana Trench, from south of Guam to the Yap Trench

  7. Mariana Arc structure inferred from gravity and seismic data

    NASA Astrophysics Data System (ADS)

    Sager, W. W.

    1980-10-01

    A two-dimensional gravity model of the lithosphere was constructed along a seismic refraction line near 18°N latitude. Included in the model are crustal layers constrained by seismic refraction results, an estimate of the gravity anomaly caused by the subducting slab, and a model of the low-density mantle beneath the Mariana Trough. With a reasonable anomaly assumed for the slab it is shown that the gravity anomaly caused by the low-density mantle is greatest over the axial bathymetric high and tapers off to the sides. With the bottom of the low-density mantle set at 200 km the density contrast is -0.033 g/cm3. Other depths and densities are tried as well. Several notable anomalies are found on the crustal layers. East of the trench, the crust has been thinned slightly to account for an outer gravity high. Behind the landward wall of the trench, a small, low-density body is modeled to explain a slight offset of the minimum of the free air anomaly from the trench axis. A 50-mGal jump on the observed gravity over the volcanic line is explained by an unusual configuration of the frontal arc Moho.

  8. Cultural alteration of human teeth in the Mariana Islands.

    PubMed

    Ikehara-Quebral, R; Douglas, M T

    1997-11-01

    Evidence of cultural dental modification in a precontact (pre-1521) skeletal sample from the Academy of Our Lady of Guam gymnasium site in Agana, Guam, is documented. Two of the four individuals recovered at the Academy Gym site exhibit modification of the maxillary teeth. One individual displays vertical incising of a single tooth, and the other exhibits horizontal abrading of the anterior teeth which may be a purposeful or an incidental alteration. Although deliberate alteration of the dentition, including tooth extraction, notching, filing, and drilling, has been documented in human groups worldwide, little has been written about these cultural practices in the Mariana Islands. Examination of the available literature on precontact human remains from the region reveals at least three patterns of dental incising and similar cases of dental abrasion. While the origins of these practices are not known, the presence and style of these cultural alterations may be sex-specific, cosmetic in nature, or an indication of status in a ranked society. Alternatively, they may signify membership in a particular group or lineage, or mark a rite of passage. Because the comparative samples are limited in number and small, and the provenience of many of the skeletons is obscure, temporal variation cannot be ruled out.

  9. A serpentinite-hosted ecosystem in the Southern Mariana Forearc.

    PubMed

    Ohara, Yasuhiko; Reagan, Mark K; Fujikura, Katsunori; Watanabe, Hiromi; Michibayashi, Katsuyoshi; Ishii, Teruaki; Stern, Robert J; Pujana, Ignacio; Martinez, Fernando; Girard, Guillaume; Ribeiro, Julia; Brounce, Maryjo; Komori, Naoaki; Kino, Masashi

    2012-02-21

    Several varieties of seafloor hydrothermal vents with widely varying fluid compositions and temperatures and vent communities occur in different tectonic settings. The discovery of the Lost City hydrothermal field in the Mid-Atlantic Ridge has stimulated interest in the role of serpentinization of peridotite in generating H(2)- and CH(4)-rich fluids and associated carbonate chimneys, as well as in the biological communities supported in highly reduced, alkaline environments. Abundant vesicomyid clam communities associated with a serpentinite-hosted hydrothermal vent system in the southern Mariana forearc were discovered during a DSV Shinkai 6500 dive in September 2010. We named this system the "Shinkai Seep Field (SSF)." The SSF appears to be a serpentinite-hosted ecosystem within a forearc (convergent margin) setting that is supported by fault-controlled fluid pathways connected to the decollement of the subducting slab. The discovery of the SSF supports the prediction that serpentinite-hosted vents may be widespread on the ocean floor. The discovery further indicates that these serpentinite-hosted low-temperature fluid vents can sustain high-biomass communities and has implications for the chemical budget of the oceans and the distribution of abyssal chemosynthetic life.

  10. A serpentinite-hosted ecosystem in the Southern Mariana Forearc

    PubMed Central

    Ohara, Yasuhiko; Reagan, Mark K.; Fujikura, Katsunori; Watanabe, Hiromi; Michibayashi, Katsuyoshi; Ishii, Teruaki; Stern, Robert J.; Pujana, Ignacio; Martinez, Fernando; Girard, Guillaume; Ribeiro, Julia; Brounce, Maryjo; Komori, Naoaki; Kino, Masashi

    2012-01-01

    Several varieties of seafloor hydrothermal vents with widely varying fluid compositions and temperatures and vent communities occur in different tectonic settings. The discovery of the Lost City hydrothermal field in the Mid-Atlantic Ridge has stimulated interest in the role of serpentinization of peridotite in generating H2- and CH4-rich fluids and associated carbonate chimneys, as well as in the biological communities supported in highly reduced, alkaline environments. Abundant vesicomyid clam communities associated with a serpentinite-hosted hydrothermal vent system in the southern Mariana forearc were discovered during a DSV Shinkai 6500 dive in September 2010. We named this system the “Shinkai Seep Field (SSF).” The SSF appears to be a serpentinite-hosted ecosystem within a forearc (convergent margin) setting that is supported by fault-controlled fluid pathways connected to the decollement of the subducting slab. The discovery of the SSF supports the prediction that serpentinite-hosted vents may be widespread on the ocean floor. The discovery further indicates that these serpentinite-hosted low-temperature fluid vents can sustain high-biomass communities and has implications for the chemical budget of the oceans and the distribution of abyssal chemosynthetic life. PMID:22323611

  11. Identification of Salt Stress Biomarkers in Romanian Carpathian Populations of Picea abies (L.) Karst.

    PubMed Central

    Schiop, Sorin T.; Al Hassan, Mohamad; Sestras, Adriana F.; Boscaiu, Monica; Sestras, Radu E.; Vicente, Oscar

    2015-01-01

    The Norway spruce (Picea abies), the most important tree species in European forests, is relatively sensitive to salt and does not grow in natural saline environments. Yet many trees are actually exposed to salt stress due to the common practice of de-icing of mountain roads in winter, using large amounts of NaCl. To help develop strategies for an appropriate use of reproductive seed material on reforestation sites, ensuring better chances of seedling survival in salt-affected areas, we have studied the responses of young spruce seedlings to salt treatments. The specific aim of the work was to identify the optimal salt stress biomarkers in Picea abies, using as experimental material seedlings obtained by germination of seeds with origin in seven populations from the Romanian Carpathian Mountains. These responses included general, conserved reactions such as the accumulation of ions and different osmolytes in the seedlings needles, reduction in photosynthetic pigments levels, or activation of antioxidant systems. Although changes in the contents of different compounds involved in these reactions can be associated to the degree of stress affecting the plants, we propose that the (decreasing) levels of total phenolics or total carotenoids and the (increasing) levels of Na+ or K+ ions in Picea abies needles, should be considered as the most reliable and useful biomarkers for salt stress in this species. They all show very high correlation with the intensity of salt stress, independently of the genetic background of the seeds parental population, and relatively easy, quantitative assays are available to determine their concentrations, requiring simple equipment and little amount of plant material. PMID:26287687

  12. Identification of Salt Stress Biomarkers in Romanian Carpathian Populations of Picea abies (L.) Karst.

    PubMed

    Schiop, Sorin T; Al Hassan, Mohamad; Sestras, Adriana F; Boscaiu, Monica; Sestras, Radu E; Vicente, Oscar

    2015-01-01

    The Norway spruce (Picea abies), the most important tree species in European forests, is relatively sensitive to salt and does not grow in natural saline environments. Yet many trees are actually exposed to salt stress due to the common practice of de-icing of mountain roads in winter, using large amounts of NaCl. To help develop strategies for an appropriate use of reproductive seed material on reforestation sites, ensuring better chances of seedling survival in salt-affected areas, we have studied the responses of young spruce seedlings to salt treatments. The specific aim of the work was to identify the optimal salt stress biomarkers in Picea abies, using as experimental material seedlings obtained by germination of seeds with origin in seven populations from the Romanian Carpathian Mountains. These responses included general, conserved reactions such as the accumulation of ions and different osmolytes in the seedlings needles, reduction in photosynthetic pigments levels, or activation of antioxidant systems. Although changes in the contents of different compounds involved in these reactions can be associated to the degree of stress affecting the plants, we propose that the (decreasing) levels of total phenolics or total carotenoids and the (increasing) levels of Na+ or K+ ions in Picea abies needles, should be considered as the most reliable and useful biomarkers for salt stress in this species. They all show very high correlation with the intensity of salt stress, independently of the genetic background of the seeds parental population, and relatively easy, quantitative assays are available to determine their concentrations, requiring simple equipment and little amount of plant material.

  13. A new phylogeny for the genus Picea from plastid, mitochondrial, and nuclear sequences.

    PubMed

    Lockwood, Jared D; Aleksić, Jelena M; Zou, Jiabin; Wang, Jing; Liu, Jianquan; Renner, Susanne S

    2013-12-01

    Studies over the past ten years have shown that the crown groups of most conifer genera are only about 15-25 Ma old. The genus Picea (spruces, Pinaceae), with around 35 species, appears to be no exception. In addition, molecular studies of co-existing spruce species have demonstrated frequent introgression. Perhaps not surprisingly therefore previous phylogenetic studies of species relationships in Picea, based mostly on plastid sequences, suffered from poor statistical support. We therefore generated mitochondrial, nuclear, and further plastid DNA sequences from carefully sourced material, striking a balance between alignability with outgroups and phylogenetic signal content. Motif duplications in mitochondrial introns were treated as characters in a stochastic Dollo model; molecular clock models were calibrated with fossils; and ancestral ranges were inferred under maximum likelihood. In agreement with previous findings, Picea diverged from its sister clade 180 million years ago (Ma), and the most recent common ancestor of today's spruces dates to 28 Ma. Different from previous analyses though, we find a large Asian clade, an American clade, and a Eurasian clade. Two expansions occurred from Asia to North America and several between Asia and Europe. Chinese P. brachytyla, American P. engelmannii, and Norway spruce, P. abies, are not monophyletic, and North America has ten, not eight species. Divergence times imply that Pleistocene refugia are unlikely to be the full explanation for the relationships between the European species and their East Asian relatives. Thus, northern Norway spruce may be part of an Asian species complex that diverged from the southern Norway spruce lineage in the Upper Miocene, some 6 Ma, which can explain the deep genetic gap noted in phylogeographic studies of Norway spruce. The large effective population sizes of spruces, and incomplete lineage sorting during speciation, mean that the interspecific relationships within each of the

  14. 20 CFR 404.1022 - American Samoa, Guam, or the Commonwealth of the Northern Mariana Islands.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... your work is not covered under Social Security, it may be covered as Medicare qualified government... the Northern Mariana Islands. 404.1022 Section 404.1022 Employees' Benefits SOCIAL SECURITY ADMINISTRATION FEDERAL OLD-AGE, SURVIVORS AND DISABILITY INSURANCE (1950- ) Employment, Wages,...

  15. Frequent excitations of T waves by earthquakes in the South Mariana Arc

    NASA Astrophysics Data System (ADS)

    Chen, Po-Fei; Chen, Kai-Xun; Cheng, Hui-Yun

    2015-02-01

    We used broadband stations in Taiwan and on the Ryukyu Arc islands to investigate T waves induced by earthquakes in the Izu-Bonin-Mariana subduction zone. Of the 48 earthquakes that took place in 2005, 17 earthquakes exhibited T-wave signals consistent with predicted arrival times at stations. Of theses T-excited events, 13 were located in the South Mariana Arc, where the isobaths exhibit strong concave curvature, and were predominantly of normal faulting type. The energies of observed T waves were used quantitatively to evaluate the relative efficiency of receiver-side acoustic-elastic conversions by Gamma calculations. Results show that the steep slopes of offshore bathymetry together with nearly perpendicular angles of back azimuth relative to local isobaths are suitable conditions for T waves observations. In 2010, two clusters of repeated moderate earthquakes in the north and south ends of the Mariana Arc displayed stark contrasts in terms of T-wave excitations despite their normal faulting type. Examining of this discrepancy indicate that a specific curvature together with a specific radiation pattern accounts for the frequent excitations of T waves from shallow earthquakes in the South Mariana Arc.

  16. Numerical experiments for evolution of backarc basins and melting processes at the Mariana subduction system

    NASA Astrophysics Data System (ADS)

    Lin, S.; Chung, S.; Kuo, B.

    2009-12-01

    Backarc basin basalts can be characterized as fertile, MORB-like end-member mixed with water-rich, previously depleted, arc-like components in various proportions. In addition, an enriched component might also contribute to the magma generation. A number of melting processes have been proposed to account for the chemical systematics of the backarc basin basalts. Previous numerical model experiments for circulation and thermal evolution in the subduction zones generally consider a steady spreading center, corresponding to mature stages. In this study we attempt to better understand the roles of different melting mechanisms and magma sources using a series of thermomechemical models of evolution of backarc basins. In the models the effects of rifting-spreading transition, ridge migration and asymmetric spreading on the thermal field, fluid distribution and melting history are incorporated. We focused on the evolution of the Mariana subduction system because the Mariana subduction zone is one of the best sampled region and its tectonic environment and history are relatively simple. Furthermore, the variations along the strike of the Mariana trough represent the sequence of the evolution of a backarc basin from rifting to mature stages of spreading. The effects of modeling parameters including slab geometry, spreading rate, velocity of subducting slab, lithospheric rheology and crustal thickness have been investigated. How the melting regimes and magma sources may evolve with time in the Mariana arc-basin system will be presented.

  17. 20 CFR 404.1022 - American Samoa, Guam, or the Commonwealth of the Northern Mariana Islands.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... by a law of the United States). The officer or employee is not considered as an employee of the... established by a law of Guam or the Commonwealth of the Northern Mariana Islands; (2) Done by an elected official; (3) Done by a member of the legislature; or (4) Done in a hospital or penal institution by...

  18. 20 CFR 404.1022 - American Samoa, Guam, or the Commonwealth of the Northern Mariana Islands.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... by a law of the United States). The officer or employee is not considered as an employee of the... established by a law of Guam or the Commonwealth of the Northern Mariana Islands; (2) Done by an elected official; (3) Done by a member of the legislature; or (4) Done in a hospital or penal institution by...

  19. 20 CFR 404.1022 - American Samoa, Guam, or the Commonwealth of the Northern Mariana Islands.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... by a law of the United States). The officer or employee is not considered as an employee of the... established by a law of Guam or the Commonwealth of the Northern Mariana Islands; (2) Done by an elected official; (3) Done by a member of the legislature; or (4) Done in a hospital or penal institution by...

  20. 20 CFR 404.1022 - American Samoa, Guam, or the Commonwealth of the Northern Mariana Islands.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... by a law of the United States). The officer or employee is not considered as an employee of the... established by a law of Guam or the Commonwealth of the Northern Mariana Islands; (2) Done by an elected official; (3) Done by a member of the legislature; or (4) Done in a hospital or penal institution by...

  1. Mosquitoes of Guam and the Northern Marianas: Distribution, Checklists, and Notes on Mosquito-Borne Pathogens

    DTIC Science & Technology

    2011-09-01

    2011). Also, a recent outbreak of Zika virus on Yap Island, Micronesia31 raised concern over its spread to Guam and other islands of the Marianas...1991 ;(suppl 3):33-39. 31. Duffy MR, Chen TH, Hancock WT, et al. Zika virus outbreak on Yap Island, Federated States of Micronesia. N Eng/ J Med

  2. 78 FR 70854 - Amendment of Restricted Area R-7201 Farallon De Medinilla Island; Mariana Islands, GU

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-27

    ... Federal Aviation Administration 14 CFR Part 73 RIN 2120-AA66 Amendment of Restricted Area R-7201 Farallon... coordinate in the boundary of restricted area R-7201, Farallon De Medinilla Island, Mariana Islands, Guam... adjustment in the longitude coordinate for R-7201 to take into account the revised positioning of FDM on...

  3. Trench-parallel flow and seismic anisotropy in the Mariana and Andean subduction systems.

    PubMed

    Kneller, Erik A; van Keken, Peter E

    2007-12-20

    Shear-wave splitting measurements above the mantle wedge of the Mariana and southern Andean subduction zones show trench-parallel seismically fast directions close to the trench and abrupt rotations to trench-perpendicular anisotropy in the back arc. These patterns of seismic anisotropy may be caused by three-dimensional flow associated with along-strike variations in slab geometry. The Mariana and Andean subduction systems are associated with the largest along-strike variations of slab geometry observed on Earth and are ideal for testing the link between slab geometry and solid-state creep processes in the mantle. Here we show, with fully three-dimensional non-newtonian subduction zone models, that the strong curvature of the Mariana slab and the transition to shallow slab dip in the Southern Andes give rise to strong trench-parallel stretching in the warm-arc and warm-back-arc mantle and to abrupt rotations in stretching directions that are accompanied by strong trench-parallel stretching. These models show that the patterns of shear-wave splitting observed in the Mariana and southern Andean systems may be caused by significant three-dimensional flow induced by along-strike variations in slab geometry.

  4. Interference of the Histone Deacetylase Inhibits Pollen Germination and Pollen Tube Growth in Picea wilsonii Mast.

    PubMed

    Cui, Yaning; Ling, Yu; Zhou, Junhui; Li, Xiaojuan

    2015-01-01

    Histone deacetylase (HDAC) is a crucial component in the regulation of gene expression in various cellular processes in animal and plant cells. HDAC has been reported to play a role in embryogenesis. However, the effect of HDAC on androgamete development remains unclear, especially in gymnosperms. In this study, we used the HDAC inhibitors trichostatin A (TSA) and sodium butyrate (NaB) to examine the role of HDAC in Picea wilsonii pollen germination and pollen tube elongation. Measurements of the tip-focused Ca2+ gradient revealed that TSA and NaB influenced this gradient. Immunofluorescence showed that actin filaments were disrupted into disorganized fragments. As a result, the vesicle trafficking was disturbed, as determined by FM4-64 labeling. Moreover, the distribution of pectins and callose in cell walls was significantly altered in response to TSA and NaB. Our results suggest that HDAC affects pollen germination and polarized pollen tube growth in Picea wilsonii by affecting the intracellular Ca2+ concentration gradient, actin organization patterns, vesicle trafficking, as well as the deposition and configuration of cell wall components.

  5. Interference of the Histone Deacetylase Inhibits Pollen Germination and Pollen Tube Growth in Picea wilsonii Mast

    PubMed Central

    Zhou, Junhui; Li, Xiaojuan

    2015-01-01

    Histone deacetylase (HDAC) is a crucial component in the regulation of gene expression in various cellular processes in animal and plant cells. HDAC has been reported to play a role in embryogenesis. However, the effect of HDAC on androgamete development remains unclear, especially in gymnosperms. In this study, we used the HDAC inhibitors trichostatin A (TSA) and sodium butyrate (NaB) to examine the role of HDAC in Picea wilsonii pollen germination and pollen tube elongation. Measurements of the tip-focused Ca2+ gradient revealed that TSA and NaB influenced this gradient. Immunofluorescence showed that actin filaments were disrupted into disorganized fragments. As a result, the vesicle trafficking was disturbed, as determined by FM4-64 labeling. Moreover, the distribution of pectins and callose in cell walls was significantly altered in response to TSA and NaB. Our results suggest that HDAC affects pollen germination and polarized pollen tube growth in Picea wilsonii by affecting the intracellular Ca2+ concentration gradient, actin organization patterns, vesicle trafficking, as well as the deposition and configuration of cell wall components. PMID:26710276

  6. A test of seven candidate barcode regions from the plastome in Picea (Pinaceae).

    PubMed

    Ran, Jin-Hua; Wang, Pei-Pei; Zhao, Hui-Juan; Wang, Xiao-Quan

    2010-12-01

    DNA barcoding, as a tool for species discrimination, has been used efficiently in animals, algae and fungi, but there are still debates on which DNA region(s) can be used as the standard barcode(s) for land plants. Gymnosperms, especially conifers, are important components of forests, and there is an urgent need for them to be identified through DNA barcoding because of their high frequency of collection in the field. However, the feasibility of DNA barcoding in gymnosperms has not been examined based on a dense species sampling. Here we selected seven candidate DNA barcodes from the plastome (matK, rbcL, rpoB, rpoC1, atpF-atpH, psbA-trnH, and psbK-psbI) to evaluate their suitability in Picea (spruce). The results showed that none of them or their different combinations has sufficient resolution for spruce species, although matK+rbcL might be used as a two-locus barcode. The low efficiency of these candidate barcodes in Picea might be caused by the paternal inheritance of the chloroplast genome, long generation time, recent radiation, and frequent inter-specific hybridization aided by wind pollination. Some of these factors could also be responsible for the difficulties in barcoding other plant groups. Furthermore, the potential of the nuclear LEAFY gene as a land plant barcode was discussed.

  7. East Mariana Basin tholeiites: Cretaceous intraplate basalts or rift basalts related to the Ontong Java plume?

    USGS Publications Warehouse

    Castillo, P.R.; Pringle, M.S.; Carlson, R.W.

    1994-01-01

    Studies of seafloor magnetic anomaly patterns suggest the presence of Jurassic oceanic crust in a large area in the western Pacific that includes the East Mariana, Nauru and Pigafetta Basins. Sampling of the igneous crust in this area by the Deep Sea Drilling Program (DSDP) and the Ocean Drilling Program (ODP) allows direct evaluation of the age and petrogenesis of this crust. ODP Leg 129 drilled a 51 m sequence of basalt pillows and massive flows in the central East Mariana Basin. 40Ar 39Ar ages determined in this study for two Leg 129 basalts average 114.6 ?? 3.2 Ma. This age is in agreement with the Albian-late Aptian paleontologic age of the overlying sediments, but is distinctively younger than the Jurassic age predicted by magnetic anomaly patterns in the basin. Compositionally, the East Mariana Basin basalts are uniformly low-K tholeiites that are depleted in highly incompatible elements compared to moderately incompatible ones, which is typical of mid-ocean ridge basalts (MORB) erupted near hotspots. The Sr, Nd and Pb isotopic compositions of the tholeiites ( 87Sr 86Srinit = 0.70360-0.70374; 143Nd 144Ndinit = 0.512769-0.512790; 206Pb 204Pbmeas = 18.355-18.386) also overlap with some Indian Ocean Ridge MORB, although they are distinct from the isotopic compositions of Jurassic basalts drilled in the Pigafetta Basin, the oldest Pacific MORB. The isotopic compositions of the East Mariana Basin tholeiites are also similar to those of intraplate basalts, and in particular, to the isotopic signature of basalts from the nearby Ontong Java and Manihiki Plateaus. The East Mariana Basin tholeiites also share many petrologic and isotopic characteristics with the oceanic basement drilled in the Nauru Basin at DSDP Site 462. In addition, the new 110.8 ?? 1.0 Ma 40Ar 39Ar age for two flows from the bottom of Site 462 in the Nauru Basin is indistinguishable from the age of the East Mariana Basin flows. Thus, while magnetic anomaly patterns predict that the igneous

  8. The Mariana Trench: A new view based on multibeam echosounding

    NASA Astrophysics Data System (ADS)

    Gardner, J. V.; Armstrong, A. A.

    2011-12-01

    The entire Mariana Trench, from its northern end at Dutton Ridge to the southwestern terminus at the Yap Trench, was mapped in 2010 using a Kongsberg EM122 12-kHz multibeam echosounder. The region ranges in depths from the shoreline at Guam to almost 11,000 m at the Challenger Deep. The northern part of the trench is receiving seamounts and guyots of the Magellan Seamount chain, whereas the southern section is receiving seafloor that carries the Caroline Ridge to the trench. The area immediately seaward of the trench where the Pacific Plate has bent downward toward the subduction zone has been broken by a series of subparallel horst and graben structures generated by extension on the bending upper surface of the Pacific Plate. Four bathymetric "bridges" span across the trench axis and extend from the Pacific Plate to the inner wall of the trench. The bridges stand as much as 2500 m above the trench axis and are composed of Latest Jurassic to Early Cretaceous accreted seamounts and guyots of the Magellan Seamount chain that are in the process of breaking up and being subducted beneath the Philippine Plate. Only two seamounts of the Caroline Ridge are in the vicinity of the trench and they both presently reside on the outer trench wall. The faults of the horsts and grabens have fractured the seamounts and guyots within the trench depression seaward from the axis outward for about 80 km, but within ~5 km of the trench axis the faults have reactivated to compressional thrust faults. The faults tend to parallel the axis of the trench until the immediate vicinity of an accreting seamount or guyot where the faults bend inward toward the trench axis, as has been observed in many other trenches. Most of the accreted seamounts and guyots are not associated with embayments or reentrants on the inner trench wall, as has been documented in the Middle America and Japan Trenches, perhaps because there is not a large accretionary prism that extends seaward of the forearc. The one

  9. H2O and CO2 in magmas from the Mariana arc and back arc systems

    NASA Astrophysics Data System (ADS)

    Newman, Sally; Stolper, Edward; Stern, Robert

    2000-05-01

    We examined the H2O and CO2 contents of glasses from lavas and xenoliths from the Mariana arc system, an intraoceanic convergent margin in the western Pacific, which contains an active volcanic arc, an actively spreading back arc basin, and active behind-the-arc cross-chain volcanoes. Samples include (1) glass rims from Mariana arc, Mariana trough, and cross-chain submarine lavas; (2) glass inclusions in arc and trough phenocrysts; and (3) glass inclusions from a gabbro + anorthosite xenolith from Agrigan (Mariana arc). Glass rims of submarine arc lavas contain 0.3-1.9 wt % H2O, and CO2 is below detection limits. Where they could be compared, glass inclusions in arc phenocrysts contain more H2O than their host glasses; most arc glasses and phenocryst inclusions contain no detectable CO2, with the exception of those from a North Hiyoshi shoshonite, which contains 400-600 ppm. The glass inclusions from the Agrigan xenolith contain 4-6% H2O, and CO2 is below the detection limit. Glasses from the cross-chain lavas are similar to those from the arc: H2O contents are 1.4-1.7 wt %, and CO2 is below detection limits. Volatile contents in Mariana trough lava glass rims are variable: 0.2-2.8 wt % H2O and 0-300 ppm CO2. Glass inclusions from trough phenocrysts have water contents similar to the host glass, but they can contain up to 875 ppm CO2. Volatile contents of melt inclusions from trough and arc lavas and from the xenolith imply minimum depths of crystallization of ~1-8 km. H2O and CO2 contents of Mariana trough glasses are negatively correlated, indicating saturation of the erupting magma with a CO2-H2O vapor at the pressure of eruption (~400 bars for these samples), with the vapor ranging from nearly pure CO2 at the CO2-rich end of the glass array to nearly pure H2O at the H2O-rich end. Degassing of these magmas on ascent and eruption leads to significant loss of CO2 (thereby masking preeruptive CO2 contents) but minimal disturbance of preeruptive H2O contents. For

  10. Variations in oceanic plate bending along the Mariana trench

    NASA Astrophysics Data System (ADS)

    Zhang, Fan; Lin, Jian; Zhan, Wenhuan

    2014-09-01

    We quantify along-trench variations in plate flexural bending along the Mariana trench in the western Pacific Ocean. A 3-D interpreted flexural deformation surface of the subducting Pacific Plate was obtained by removing from the observed bathymetry the effects of sediment loading, isostatically-compensated topography based on gravity modeling, age-related lithospheric thermal subsidence, and residual short-wavelength features. We analyzed flexural bending of 75 across-trench profile sections and calculated five best-fitting tectonic and plate parameters that control the flexural bending. Results of analysis revealed significant along-trench variations: the trench relief varies from 0.9 to 5.7 km, trench-axis vertical loading (-V0) from -0.73×1012 to 3.17×1012 N/m, and axial bending moment (-M0) from 0.1×1017 to 2.7×1017 N. The effective elastic plate thickness seaward of the outer-rise region (TeM) ranges from 45 to 52 km, while that trench-ward of the outer-rise (Tem) ranges from 19 to 40 km. This corresponds to a reduction in Te of 21-61%. The transition from TeM to Tem occurs at a breaking distance of 60-125 km from the trench axis, which is near the outer-rise and corresponds to the onset of observed pervasive normal faults. The Challenger Deep area is associated with the greatest trench relief and axial vertical loading, while areas with seamounts at the trench axis are often associated with more subtle trench relief, smaller axial vertical loading, and greater topographic bulge at the outer-rise.

  11. Constraints upon water advection in sediments of the Mariana Trough

    SciTech Connect

    Abbott, D.H.; Menke, W.; Morin, R.

    1983-02-10

    Thermal gradient measurements, consolidation tests, and pore water compositions from the Mariana Trough imply that water is moving through the sediments in areas with less than about 100 m of sediment cover. The maximum advection rates implied by the thermal measurements and consolidation tests may be as high as 10/sup -5/ cm s/sup -1/ but are most commonly in the range of 1 to 5 x 10/sup -6/ cm s/sup -1/. Theoretical calculations of the effect of the highest advection rates upon carbonate dissolution indicate that dissolution may be impeded or enhanced (depending upon the direction of flow) by a factor of 2 to 5 times the rate for diffusion alone. The average percentage of carbonate is consistently higher in two cores from the area with no advection or upward advection than the average percentage of carbonate in three cores from the area with downward advection. This increase in average amount of carbonate in cores with upward moving water or no movement cannot be attributed solely to differences in water depth or in amount of terrigenous dilution. If the sediment column acts as a passive boundary layer, then the water velocities necessary to affect chemical gradients of silica are in the range 10/sup -9/ to 10/sup -10/ cm s/sup -1/. However, if dissolution of silica occurs within the sediment column, then the advection velocities needed to affect chemical gradients are at least 3 x 10/sup -8/ cm s/sup -1/ and may be as high as 3 x 10/sup -6/ cm s/sup -1/. This order of magnitude increase in advection velocities when chemical reactions occur within the sediments is probably applicable to other cations in addition to silica. If so, then the advection velocities needed to affect heat flow (>10/sup -8/ cm s/sup -1/) and pore water chemical gradients are much nearer in magnitude than previously assumed.

  12. Recombinant sterol esterase from Ophiostoma piceae: an improved biocatalyst expressed in Pichia pastoris

    PubMed Central

    2012-01-01

    Background The ascomycete Ophiostoma piceae produces a sterol esterase (OPE) with high affinity towards p-nitrophenol, glycerol and sterol esters. Its hydrolytic activity on natural mixtures of triglycerides and sterol esters has been proposed for pitch biocontrol in paper industry since these compounds produce important economic losses during paper pulp manufacture. Results Recently, this enzyme has been heterologously expressed in the methylotrophic yeast Pichia pastoris, and the hydrolytic activity of the recombinant protein (OPE*) studied. After the initial screening of different clones expressing the enzyme, only one was selected for showing the highest production rate. Different culture conditions were tested to improve the expression of the recombinant enzyme. Complex media were better than minimal media for production, but in any case the levels of enzymatic activity were higher (7-fold in the best case) than those obtained from O. piceae. The purified enzyme had a molecular mass of 76 kDa, higher than that reported for the native enzyme under SDS-PAGE (60 kDa). Steady-state kinetic characterization of the recombinant protein showed improved catalytic efficiency for this enzyme as compared to the native one, for all the assayed substrates (p-nitrophenol, glycerol, and cholesterol esters). Different causes for this were studied, as the increased glycosylation degree of the recombinant enzyme, their secondary structures or the oxidation of methionine residues. However, none of these could explain the improvements found in the recombinant protein. N-terminal sequencing of OPE* showed that two populations of this enzyme were expressed, having either 6 or 8 amino acid residues more than the native one. This fact affected the aggregation behaviour of the recombinant protein, as was corroborated by analytical ultracentrifugation, thus improving the catalytic efficiency of this enzyme. Conclusion P. pastoris resulted to be an optimum biofactory for the

  13. Two novel techniques to screen Abies seedlings for resistance to the balsam woolly adelgid, Adelges piceae.

    PubMed

    Newton, Leslie; Frampton, John; Monahan, John; Goldfarb, Barry; Hain, Fred

    2011-01-01

    Since its introduction into the Southern Appalachians in the 1950s, the balsam woolly adelgid, Adelges piceae Ratzeburg (Hemiptera: Adelgidae), has devastated native populations of Fraser fir, Abies fraseri (Pursh) Poir. (Pinales: Pinaceae), and has become a major pest in Christmas tree plantations requiring expensive chemical treatments. Adelges piceae-resistant Fraser fir trees would lessen costs for the Christmas tree industry and assist in the restoration of native stands. Resistance screening is an important step in this process. Here, four studies directed toward the development of time- and cost-efficient techniques for screening are reported. In the first study, three methods to artificially infest seedlings of different ages were evaluated in a shade-covered greenhouse. Two-year-old seedlings had much lower infestation levels than 7 year-old seedlings. Placing infested bark at the base of the seedling was less effective than tying infested bark to the seedling or suspending infested bolts above the seedling. Although the two latter techniques resulted in similar densities on the seedlings, they each have positive and negative considerations. Attaching bark to uninfested trees is effective, but very time consuming. The suspended bolt method mimics natural infestation and is more economical than attaching bark, but care must be taken to ensure an even distribution of crawlers falling onto the seedlings. The second study focused on the density and distribution of crawlers falling from suspended bolts onto paper gridded into 7.6 × 7.6 cm cells. Crawler density in a 30 cm band under and to each side of the suspended bolt ranged from 400 to over 3000 crawlers per cell (1 to 55 crawlers per cm²). In the third study, excised branches from 4 year-old A. fraseri and A. vetchii seedlings were artificially infested with A. piceae to determine whether this technique may be useful for early resistance screening. The excised A. fraseri branches supported complete

  14. Higher thermal acclimation potential of respiration but not photosynthesis in two alpine Picea taxa in contrast to two lowland congeners.

    PubMed

    Zhang, Xiao Wei; Wang, Jing Ru; Ji, Ming Fei; Milne, Richard Ian; Wang, Ming Hao; Liu, Jian-Quan; Shi, Sheng; Yang, Shu-Li; Zhao, Chang-Ming

    2015-01-01

    The members of the genus Picea form a dominant component in many alpine and boreal forests which are the major sink for atmospheric CO2. However, little is known about the growth response and acclimation of CO2 exchange characteristics to high temperature stress in Picea taxa from different altitudes. Gas exchange parameters and growth characteristics were recorded from four year old seedlings of two alpine (Picea likiangensis vars. rubescens and linzhiensis) and two lowland (P. koraiensis and P. meyeri) taxa. Seedlings were grown at moderate (25°C/15°C) and high (35°C/25°C) day/night temperatures, for four months. The approximated biomass increment (ΔD2H) for all taxa decreased under high temperature stress, associated with decreased photosynthesis and increased respiration. However, the two alpine taxa exhibited lower photosynthetic acclimation and higher respiratory acclimation than either lowland taxon. Moreover, higher leaf dry mass per unit area (LMA) and leaf nitrogen content per unit area (Narea), and a smaller change in the nitrogen use efficiency of photosynthesis (PNUE) for lowland taxa indicated that these maintained higher homeostasis of photosynthesis than alpine taxa. The higher respiration rates produced more energy for repair and maintenance biomass, especially for higher photosynthetic activity for lowland taxa, which causes lower respiratory acclimation. Thus, the changes of ΔD2H for alpine spruces were larger than that for lowland spruces. These results indicate that long term heat stress negatively impact on the growth of Picea seedlings, and alpine taxa are more affected than low altitude ones by high temperature stress. Hence the altitude ranges of Picea taxa should be taken into account when predicting changes to carbon fluxes in warmer conditions.

  15. Recent Results of Hadal Investigations in the Southern Mariana Trench

    NASA Astrophysics Data System (ADS)

    Fryer, P. B.; Hellebrand, E.; Sharma, S. K.; Acosta-Maeda, T.; Jicha, B. R.; Cameron, J.

    2014-12-01

    The deepest parts of the southern Mariana Trench have variously been interpreted to 1) indicate strike-slip motion along the trench, 2) contain a series of 3 sediment ponds at greater than 10,900 m depth separated from one another by fault-controlled ridges on the subducting plate, and 3) have an even deeper feature in the western-most pond (Vitiaz Deep). Recent lander deployments in all three ponds and the Deepsea Challenger submersible dive by J. Cameron in 2012 showed that the deepest ponds within the Challenger Deep area have nearly unbroken, flat surfaces. One point explored showed veined serpentinite at a depth of 10,800+ m. The potential for active serpentinite-hosted seeps and vent communities was demonstrated for the Shinkai Vent Field at 5,800m depth. Rocks collected using the Wood Hole Oceanographic Institution's hybrid remotely operated vehicle, Nereus, in 2009 from deep (10,879 m) on the incoming plate south of the Challenger Deep, were recovered from the base of a fault scarp where large, columnar-jointed blocks are draped with sediment. Optical microscopy, electron-microprobe and Raman analysis show that they are partially altered massive diabase with altered interstitial glass and containing microbial tubules in vug-filling secondary phases. The chain of seamounts striking NNW, colinear with the Lyra Trough, has been interpreted as a boundary between the Pacific Plate and the seafloor north of the Caroline Ridge. Sediments, drilled from above postulated basement north of the Caroline Ridge are no older that Oligocene. Ar/Ar age dates completed for one rock collected by Nereus in 2009 give a weighted mean plateau age, based on two experiments, of 24.6 +/- 3.2 Ma. Thus, the igneous basement of the subducting plate south of the Challenger Deep is, far younger than the Jurassic Pacific Plate subducting further east. This represents a previously unidentified tectonic plate. With new vehicles and technologies the future for hadal exploration is ripe.

  16. The 2014 Submarine Eruption of Ahyi Volcano, Northern Mariana Islands

    NASA Astrophysics Data System (ADS)

    Haney, M. M.; Chadwick, W.; Merle, S. G.; Buck, N. J.; Butterfield, D. A.; Coombs, M. L.; Evers, L. G.; Heaney, K. D.; Lyons, J. J.; Searcy, C. K.; Walker, S. L.; Young, C.; Embley, R. W.

    2014-12-01

    On April 23, 2014, Ahyi Volcano, a submarine cone in the Northern Mariana Islands (NMI), ended a 13-year-long period of repose with an explosive eruption lasting over 2 weeks. The remoteness of the volcano and the presence of several seamounts in the immediate area posed a challenge for constraining the source location of the eruption. Critical to honing in on the Ahyi area quickly were quantitative error estimates provided by the CTBTO on the backazimuth of hydroacoustic arrivals observed at Wake Island (IMS station H11). T-phases registered across the NMI seismic network at the rate of approximately 10 per hour until May 8 and were observed in hindsight at seismic stations on Guam and Chichijima. After May 8, sporadic T-phases were observed until May 17. Within days of the eruption onset, reports were received from NOAA research divers of hearing explosions underwater and through the hull on the ship while working on the SE coastline of Farallon de Pajaros (Uracas), a distance of 20 km NW of Ahyi. In the same area, the NOAA crew reported sighting mats of orange-yellow bubbles on the water surface and extending up to 1 km from the shoreline. Despite these observations, satellite images showed nothing unusual throughout the eruption. During mid-May, a later cruise leg on the NOAA ship Hi'ialakai that was previously scheduled in the Ahyi area was able to collect some additional data in response to the eruption. Preliminary multibeam sonar bathymetry and water-column CTD casts were obtained at Ahyi. Comparison between 2003 and 2014 bathymetry revealed that the minimum depth had changed from 60 m in 2003 to 75 m in 2014, and a new crater ~95 m deep had formed at the summit. Extending SSE from the crater was a new scoured-out landslide chute extending downslope to a depth of at least 2300 m. Up to 125 m of material had been removed from the head of the landslide chute and downslope deposits were up to 40 m thick. Significant particle plumes were detected at all three

  17. Mantle Heterogeneity in the Southern Mariana Trough Indicated Through B and Sr Isotopic Systematics.

    NASA Astrophysics Data System (ADS)

    Kohut, E. J.; Savov, I.

    2008-12-01

    We have studied glasses from the Southernmost Mariana Trough (13.4-14.3° N and 144-144.6° E) recovered during the Cook 7 expedition (2001). Due to their differing proximities to the volcanic front of the Mariana island arc (33 to 106 km), these samples are critical for evaluating across arc elemental and isotope transport and possible linkages between back-arc spreading ridges and the adjacent volcanic arc chains. Petrographic examination shows all of the samples to be unaltered dark colored volcanic glasses with rare crystals of olivine, pyroxene and plagioclase. Their Mg #s range between 33 and 60, with most Mg# >50. Although samples were recovered from water depths of 3.2 to 4.2 km, there is lack of major element and Cl/K concentrations that indicate any seawater alterations (e.g. LOI and Na contents). The lack of seawater signature is also shown by low (0.70275 to 0.70302) 87Sr/86Sr, which is depleted MORB range. The glasses also have low H2O (~1 wt%). This lack of secondary glass alteration allows study of the behavior of volatile and low- temperature alteration sensitive tracers (e.g. B, Cl and B and Sr isotopes). There is no correlation between depth of recovery and B, Cl or d11B systematics, indicating that the degree of vesiculation did not control the d11B. The B content of the glasses is low (generally MORB- like) and the Cl contents show progressive increase with distance from the volcanic front and range between 150 and 1300 ppm. The d11B ratios vary widely and are between +5.3 to -5.6 per mil. As expected, the d11B values become progressively heavier closer to the volcanic front, where they are indistinguishable from the Mariana arc lavas. Interestingly, the heaviest d11B samples are also associated with elevated ratios of Ba/La and U/Nb, and to some extent also with elevated Th/Yb. In arcs, U and Ba are fluid mobile, while Th is a sediment melt sensitive element. From the preliminary dataset it appears that the samples from the S. Mariana Trough

  18. Seismogenic characteristics of the Mariana shallow thrust zone: What controls plate coupling?

    NASA Astrophysics Data System (ADS)

    Wiens, D. A.; Emry, E.; Shiobara, H.; Sugioka, H.

    2007-05-01

    The Mariana arc has been the type example of an "aseismic" or "decoupled" subduction zone since the concept was first proposed by Uyeda and Kanamori [1979]. Most past studies of the seismogenic zone characteristics have focused on "coupled" subduction zones that show large thrust earthquakes. Comparative studies of "decoupled" zones are essential for understanding the factors controlling the occurrence of large earthquakes. We use land and ocean bottom seismograph records from the 2003-2004 Mariana Subduction Factory Imaging Experiment to study the seismicity of the Mariana forearc. This 11 month experiment consisted of 20 broadband stations deployed on islands and 58 semi-broadband ocean bottom seismographs (OBS), with 14 OBSs located in the forearc. Events were detected and arrival times picked with Antelope package and relocated with a hypocentroidal decomposition relative location method. Overall, we have located ~ 500 events in the Mariana forearc between 16-19 N with mb 2 - 5. We determined the focal mechanisms of the largest events using a regional waveform inversion method. We have recently also detected episodic tremor that may be similar to that found at Cascadia, Japan, and several other subduction zones. The results indicate a lack of significant seismicity in the outer forearc beneath Big Blue and Celestial serpentinite seamounts. A few well-located events suggest that the shallow thrust zone is located at a depth of 15-25 km beneath the seamounts. Patches of high seismicity in the shallow thrust zone are located to the west of the seamounts at depths of 30-40 km. The patches are approximately 20 km in diameter and suggest heterogeneous faulting properties along the shallow thrust zone. Another zone of earthquakes at depths of about 60 km initiate beneath the seamounts and extend towards the west, representing faulting within the subducting plate. These events indicate that the lower zone of the Mariana double seismic zone initiates in the forearc and

  19. Geochemistry of manganese nodules from offshore areas of Mariana Islands and Johnston Island

    NASA Astrophysics Data System (ADS)

    Chen, Ju-chin; Yao, Yung-chang

    The manganese nodules near the Mariana Islands generally range from 2 to 4 cm in diameter and some samples have porous surfaces. The nodules near Johnston Island are larger in size (5-8 cm in diameter) and more compact than the Mariana nodules. The major FeMn minerals found in Mariana Islands samples are todorokite, birnessite and akaganeite (β-FeOOH) while, in the Johnston Island samples, only birnessite and akaganeite are found. The Mariana Islands nodules are higher in Mn, Mg, Na, K, Co, Ni, Pb and Th but lower in Fe, Sr, Zn, Ba, Zr, Y and REEs than the Johnston Island samples. The (Cu + Ni + Co) contents of the Mariana Islands samples are generally higher than 15,000 ppm with {Co}/{Zn} ratios varying from 10 to 15, while the Johnston Island samples generally have (Cu + Ni + Co) between 8000 and 10,000 ppm with {Co}/{Zn} ratios varying from 5 to 7. Therefore these nodules may not be related to hydrothermal activity (Toth, Geol. Soc. Am. Bull.91, 44-54, 1980). Both the Mariana Islands and Johnston Island nodules show similar LREE-enriched patterns with distinct positive Ce anomaly and negative Eu anomaly. The positive correlation between the Ce anomaly defined as log {Ce}/{{2}/{3}La+ {1}/{3}Nd } and {Mn}/{Fe} ratios found in the nodules studied suggest that a phosphorus-rich phase with REE pattern similar to that for biogenous apatite may be the third component in considering the source of REEs in the nodules. The growth rate determined by the excess 230Th method for Johnston Island nodule JA-1, from 0.2 to 0.45 mm depth, is 1.12±0.10 mm/Ma but a much higher rate (17.66 ± 7.61 mm/Ma) is observed from 0.45 to 1.8 mm depth. The growth rate of the nodule may be related to its Mn and Fe contents.

  20. Ophiostoma kryptum sp. nov. from Larix decidua and Picea abies in Europe, similar to O. minus.

    PubMed

    Jacobs, Karin; Kirisits, Thomas

    2003-10-01

    An unknown species of Ophiostoma was isolated from European larch (Larix decidua) infested by Tetropium gabrieli (Coleoptera: Cerambycidae) and Norway spruce (Picea abies) infested by Tetropium sp. in Austria. The fungus is similar to O. minus, but distinguished from it by the ecology, colony morphologies on OA and MEA, and phylogenetic analysis of aligned DNA sequences of the ITS region of the rDNA operon and the partial beta-tubulin gene. It is described here as O. kryptum sp. nov. The new species readily produces perithecia with short necks and reniform ascospores, and has Hyalorhinocladiella and Leptographium-like anamorphs. Circumstantial evidence suggests that Tetropium spp. act as vectors of O. kryptum. O. minus and O. kryptum represent additional examples of morphologically similar, yet genetically and ecologically distinct species in the genus Ophiostoma. The new combination, O. crenulatum comb. nov. (syn. CeratocYstiopsis crenulata), is also made.

  1. In vitro fungistatic effects of natural coniferous resin from Norway spruce (Picea abies).

    PubMed

    Rautio, M; Sipponen, A; Lohi, J; Lounatmaa, K; Koukila-Kähkölä, P; Laitinen, K

    2012-08-01

    Resins (rosin, pitch) are natural products of the coniferous trees and are antimicrobial against a wide range of microbes. The antifungal effectiveness of resin, purified from Norway spruce (Picea abies), was studied against human pathogenic fungi and yeasts with the agar plate diffusion tests and electron microscopy (EM). The fungistatic effect of these resin mixtures (resin salves) was tested against a set of Candida yeasts, dermatophytes, and opportunistic fungi. Transmission and scanning EM was done from samples of fungi (Trichophyton mentagrophytes). In agar diffusion tests, the resin was strongly antifungal against all dermatophytes tested, e.g., against all fungi of the genus Trichophyton, but it was not antifungal against the Candida yeasts or against the opportunistic fungi tested. According to EM, resin caused damages in the cell hyphae and cell wall structures. We conclude that, in the agar plate diffusion test, coniferous resins are strongly fungistatic against the dermatophytic fungi only.

  2. Serendipitous Meta-Transcriptomics: The Fungal Community of Norway Spruce (Picea abies).

    PubMed

    Delhomme, Nicolas; Sundström, Görel; Zamani, Neda; Lantz, Henrik; Lin, Yao-Cheng; Hvidsten, Torgeir R; Höppner, Marc P; Jern, Patric; Van de Peer, Yves; Lundeberg, Joakim; Grabherr, Manfred G; Street, Nathaniel R

    2015-01-01

    After performing de novo transcript assembly of >1 billion RNA-Sequencing reads obtained from 22 samples of different Norway spruce (Picea abies) tissues that were not surface sterilized, we found that assembled sequences captured a mix of plant, lichen, and fungal transcripts. The latter were likely expressed by endophytic and epiphytic symbionts, indicating that these organisms were present, alive, and metabolically active. Here, we show that these serendipitously sequenced transcripts need not be considered merely as contamination, as is common, but that they provide insight into the plant's phyllosphere. Notably, we could classify these transcripts as originating predominantly from Dothideomycetes and Leotiomycetes species, with functional annotation of gene families indicating active growth and metabolism, with particular regards to glucose intake and processing, as well as gene regulation.

  3. No time for spruce: rapid dampening of circadian rhythms in Picea abies (L. Karst).

    PubMed

    Gyllenstrand, Niclas; Karlgren, Anna; Clapham, David; Holm, Karl; Hall, Anthony; Gould, Peter D; Källman, Thomas; Lagercrantz, Ulf

    2014-03-01

    The identification and cloning of full-length homologs of circadian clock genes from Picea abies represent a first step to study the function and evolution of the circadian clock in gymnosperms. Phylogenetic analyses suggest that the sequences of key circadian clock genes are conserved between angiosperms and gymnosperms. though fewer homologous copies were found for most gene families in P. abies. We detected diurnal cycling of circadian clock genes in P. abies using quantitative real-time PCR; however, cycling appeared to be rapidly dampened under free-running conditions. Given the unexpected absence of transcriptional cycling during constant conditions, we employed a complementary method to assay circadian rhythmic outputs and measured delayed fluorescence in seedlings of Norway spruce. Neither of the two approaches to study circadian rhythms in Norway spruce could detect robust ∼24 h cycling behavior under constant conditions. These data suggest gene conservation but fundamental differences in clock function between gymnosperms and other plant taxa.

  4. [Water storage capacity of qinghai spruce (Picea crassifolia) forest canopy in Qilian Mountains].

    PubMed

    Peng, Huan-hua; Zhao, Chuan-yan; Xu, Zhong-lin; Peng, Shou-zhang; Wang, Yao

    2011-09-01

    By the methods of direct measurement and regression analysis, this paper estimated the water storage capacity of Picea crassifolia forest canopy in Guantan in Qilianshan Mountains, based on the observed throughfall and the laboratory experimental data about the water storage capacity of various canopy components in 2008. Due to the impacts of various factors, differences existed in the canopy water storage capacity estimated by the two methods. The regression analysis was mainly impacted by the measurement approaches of the throughfall, the maximum water storage capacity estimated being 0.69 mm, whereas the direct measurement was mainly impacted by tree height, diameter at breast height, plant density, and leaf area index, with the estimated maximum water storage capacity being 0.77 mm. The direct measurement showed that the maximum water storage capacity per unit area of the canopy components of the forest was in the order of barks (0.31 mm) > branches (0.28 mm) > leaves (0.08 mm).

  5. Ectomycorrhizal specificity patterns in a mixed Pinus contorta and Picea engelmannii forest in Yellowstone National Park

    NASA Technical Reports Server (NTRS)

    Cullings, K. W.; Vogler, D. R.; Parker, V. T.; Finley, S. K.

    2000-01-01

    We used molecular genetic methods to test two hypotheses, (i) that host plant specificity among ectomycorrhizal fungi would be common in a closed-canopy, mixed Pinus contorta-Picea engelmannii forest in Yellowstone National Park and (ii) that specificity would be more common in the early successional tree species, P. contorta, than in the invader, P. engelmannii. We identified 28 ectomycorrhizal fungal species collected from 27 soil cores. The proportion of P. engelmannii to P. contorta ectomycorrhizae was nearly equal (52 and 48%, respectively). Of the 28 fungal species, 18 composed greater than 95% of the fungal community. No species was associated exclusively with P. contorta, but four species, each found in only one core, and one species found in two cores were associated exclusively with P. engelmannii. These fungi composed less than 5% of the total ectomycorrhizae. Thus, neither hypothesis was supported, and hypothesized benefits of ectomycorrhizal specificity to both trees and fungi probably do not exist in this system.

  6. Antibacterial effects of home-made resin salve from Norway spruce (Picea abies).

    PubMed

    Rautio, M; Sipponen, A; Peltola, R; Lohi, J; Jokinen, J J; Papp, A; Carlson, P; Sipponen, P

    2007-04-01

    Resin salve made from Norway spruce (Picea abies) is traditionally used in folk medicine to heal skin ulcers and infected wounds. Its antimicrobial properties were studied against certain human bacteria important in infected skin wounds. The sensitivity of the resin against Gram-positive and Gram-negative bacteria was studied in vitro by methods that are routinely used in microbiology laboratories. The resin salve exhibited a bacteriostatic effect against all tested Gram-positive bacteria but only against Proteus vulgaris of the Gram-negative bacteria. Interestingly, the resin inhibited the growth of bacteria, including methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant enterococcus (VRE), both on agar plates and in culture media. The study demonstrated antimicrobial activity of the resin salve and provided objective evidence of its antimicrobial properties. It gives some explanations why the traditional use of home-made resin salve from Norway spruce is experienced as being effective in the treatment of infected skin ulcers.

  7. Growing trees on completed sanitary landfills. [Nyssa sylvatica, Picea abies, Ginkgo biloba

    SciTech Connect

    Leone, I.A.; Gilman, E.F.; Flower, F.B.

    1983-01-01

    A 10-year old completed landfill in New Jersey consisting of 9 m (depth) of refuse covered with 15-25 cm of soil was cleared of debris and vegetation and covered with 30 cm of subsoil and 15-25 cm of topsoil. Nineteen coniferous and broadleaved species were planted on the landfill and on a control site in 1975, and trees were maintained and growth and condition monitored over 4 years. On the basis of shoot length and stem area increase, the most successful of the surviving trees were Nyssa sylvatica, Picea abies and Ginkgo biloba, in decreasing order of tolerance. Tolerance of landfill conditions appeared to be greatest in those species with low water requirements, a slow growth rate, high acid tolerance and a shallow root system. (Refs. 11).

  8. Impact of eastern dwarf mistletoe (Arceuthobium pusillum) infection on the needles of red spruce (Picea rubens) and white spruce (Picea glauca): oxygen exchange, morphology and composition.

    PubMed

    Reblin, Jaret S; Logan, Barry A; Tissue, David T

    2006-10-01

    Eastern dwarf mistletoe (Arceuthobium pusillum Peck) is a hemiparasitic angiosperm that infects white spruce (Picea glauca (Moench) Voss) and red spruce (P. rubens Sarg.) in northeastern North America. The effects of mistletoe infection differ substantially between white and red spruce, with white spruce suffering greater infection-induced mortality. In the present study, we sought to determine the role that species-specific differences in needle-scale responses to parasitism may play in the observed differences in the effect of infection on host tree health. Based on the measurements made, the most apparent effect of parasitism was a reduction in needle size distal to infections. The magnitude of this reduction was greater in white spruce than in red spruce. Eastern dwarf mistletoe was a sink for host photosynthate in red spruce and white spruce; however, there were no adjustments in needle photosynthetic capacities in either host to accommodate the added sink demands of the parasite. Needle total nonstructural carbohydrate concentrations (TNC) were also unaltered by infection. Red spruce needles had higher TNC concentrations despite having lower overall photosynthetic capacities, suggesting that red spruce may be more sink limited and therefore better able to satisfy the added sink demands of parasitic infection. However, if carbon availability limits the growth of the mistletoe, one may expect that the extent of the parasitic infection would be greater in red spruce. Yet in the field, the extent of infection is generally greater in white spruce. Taken together, these results suggest that dwarf mistletoe may not substantially perturb the carbon balance of either host spruce species and that species-specific differences in needle-scale responses to the parasite cannot explain the contrasting effects of infection on white spruce and red spruce.

  9. Geology of Saipan, Mariana Islands; Part 4, Submarine topography and shoal-water ecology

    USGS Publications Warehouse

    Cloud, Preston E.

    1959-01-01

    The topography of the sea floor within 10 miles of Saipan broadly resembles that of the land. Eastward, toward the Mariana trench, slopes are about 6°, without prominent benches or scarps. This is inferred to indicate easterly continuation of generally pyroclastic bedrock. The westward slope averages 2° to 3° and consists mainly of nearly flat benches and westfacing scarps. This is taken to imply westward continuation of a limestone bench-and-fault-scarp topography. Projection of known faults to sea and through Tinian, on the basis of topographic trends, suggests a pattern of west-dipping normal faults that parallel the strike of the Mariana ridge and affect the shape and position of islands at the crest of the ridge.

  10. MARGINS mini-lessons: A tour of the Mariana Subduction System (Invited)

    NASA Astrophysics Data System (ADS)

    Goodliffe, A. M.; Oakley, A.

    2009-12-01

    MARGINS mini-lessons provide an efficient way to quickly move cutting edge MARGINS research into the university classroom. Instructors who are not necessarily familiar with the MARGINS program can easily use mini-lessons in a variety of educational settings. The mini-lesson described herein is centered on bathymetric and multi-channel seismic data collected during a 2003 NSF-MARGINS funded marine geophysical survey in the Mariana Basin. Designed as an approximately sixty minute lecture segment, the lesson covers both the techniques used to collect marine geophysical data and a description of the geology of the system. All geological provinces are included, from the subducting Pacific Plate in the east to the remnant arc in the west. Representative seismic lines and bathymetric images are presented for each province, along with a description of key processes including deformation of the subducting plate, serpentinite mud volcanism, forearc faulting, potentially tsunamigenic landslides, arc volcanism, and backarc spreading. The Mariana subduction system mini-lesson requires a computer with an internet connection, powerpoint, Google Earth, and a web-browser. Questions are embedded in the powerpoint presentation that can be adapted to a specific interactive response system as needed. Optimally the lesson should be used in parallel with a GeoWall. A 3-dimensional ArcScene visualization of the Mariana system is available for download through the MARGINS mini-lessons web site. Such visualizations are particularly effective in helping students understand complex three-dimensional systems. If presented in a computer lab students will benefit from being able to explore the Mariana system using tools such as GeoMapApp.

  11. Constraining kinetics of metastable olivine in the Marianas slab from seismic observations and dynamic models

    NASA Astrophysics Data System (ADS)

    Quinteros, Javier; Sobolev, Stephan V.

    2012-03-01

    Transformation kinetics associated with the presence of a metastable olivine wedge in old and fast subducting slabs has been the subject of many studies in the last years. Even with improvements in kinetics models, many of the parameters are still not well constrained. In particular, there is no consensus on the blocking temperature that could inhibit the transformation from olivine to spinel. Recently, based on anomalous later phases in the P wave coda and differential P wave slowness, a wedge of metastable olivine was detected in the Marianas subduction zone, that is approximately 25 km wide, at a depth of 590 km and is truncated at 630 km. In this work, a thermomechanical model was used to mimic the subduction in the Marianas and try different blocking temperatures for the olivine/spinel transformation. The model includes, among other features, non-linear elasto-visco-plastic rheology based on laboratory data, phase transformations, latent heat, proper coupling between stress and thermal state of the slab and force balance of the system. The results show a positive correlation between the blocking temperature, depth of the wedge and its distance from the trench (or subduction angle). We compare these results to the situation in the Marianas and suggest that a blocking temperature for the olivine/spinel transformation of approximately 725 °C would be the most likely. The volume of the wedge presents some oscillations that we relate to a runaway effect of the transformation kinetics in the mantle transition zone. Namely, the interaction between latent heat release and the advection of the isotherms due to the subduction velocity. The inclusion of shear heating in the model was fundamental to modeling such a subduction zone. Without shear heating, the slab shows a higher level of internal stress and the necessary bending to mimic the Marianas subduction zone cannot be reached.

  12. The Fina Nagu volcanic complex: Unusual submarine arc volcanism in the rapidly deforming southern Mariana margin

    NASA Astrophysics Data System (ADS)

    Brounce, Maryjo; Kelley, Katherine A.; Stern, Robert; Martinez, Fernando; Cottrell, Elizabeth

    2016-10-01

    In the Mariana convergent margin, large arc volcanoes disappear south of Guam even though the Pacific plate continues to subduct and instead, small cones scatter on the seafloor. These small cones could form either due to decompression melting accompanying back-arc extension or flux melting, as expected for arc volcanoes, or as a result of both processes. Here, we report the major, trace, and volatile element compositions, as well as the oxidation state of Fe, in recently dredged, fresh pillow lavas from the Fina Nagu volcanic chain, an unusual alignment of small, closely spaced submarine calderas and cones southwest of Guam. We show that Fina Nagu magmas are the consequence of mantle melting due to infiltrating aqueous fluids and sediment melts sourced from the subducting Pacific plate into a depleted mantle wedge, similar in extent of melting to accepted models for arc melts. Fina Nagu magmas are not as oxidized as magmas elsewhere along the Mariana arc, suggesting that the subduction component responsible for producing arc magmas is either different or not present in the zone of melt generation for Fina Nagu, and that amphibole or serpentine mineral destabilization reactions are key in producing oxidized arc magmas. Individual Fina Nagu volcanic structures are smaller in volume than Mariana arc volcanoes, although the estimated cumulative volume of the volcanic chain is similar to nearby submarine arc volcanoes. We conclude that melt generation under the Fina Nagu chain occurs by similar mechanisms as under Mariana arc volcanoes, but that complex lithospheric deformation in the region distributes the melts among several small edifices that get younger to the northeast.

  13. Multi-Channel Seismic Images of the Mariana Forearc: EW0202 Initial Results

    NASA Astrophysics Data System (ADS)

    Oakley, A. J.; Goodliffe, A. M.; Taylor, B.; Moore, G. F.; Fryer, P.

    2002-12-01

    During the Spring of 2002, the Mariana Subduction Factory was surveyed using multi-channel seismics (MCS) as the first major phase of a US-Japanese collaborative NSF-MARGINS funded project. The resulting geophysical transects extend from the Pacific Plate to the West Mariana remnant arc. For details of this survey, including the results from the back-arc, refer to Taylor et al. (this session). The incoming Pacific Plate and its accompanying seamounts are deformed by plate flexure, resulting in extension of the upper crust as it enters the subduction zone. The resultant trench parallel faults dominate the bathymetry and MCS data. Beneath the forearc, in the southern transects near Saipan, the subducting slab is imaged to a distance of 50-60 km arcward. In addition to ubiquitous trench parallel normal faulting, a N-S transect of the forearc clearly shows normal faults perpendicular to the trench resulting from N-S extension. On the east side of the Mariana Ridge, thick sediment packages extend into the forearc. Directly east of Saipan and Tinian, a large, deeply scouring slide mass is imaged. Several serpentine mud volcanoes (Big Blue, Turquoise and Celestial) were imaged on the Mariana Forearc. Deep horizontal reflectors (likely original forearc crust) are imaged under the flanks of some of these seamounts. A possible "throat" reflector is resolved on multiple profiles at the summit of Big Blue, the northern-most seamount in the study area. The flanks of Turquoise seamount terminate in toe thrusts that represent uplift and rotation of surrounding sediments as the volcano grows outward. These thrusts form a basal ridge around the seamount similar to that previously noted encircling Conical Seamount. Furthermore, MCS data has revealed that some forearc highs previously thought to be fault blocks are in actuality mud volcanoes.

  14. 8 CFR 233.6 - Aliens entering Guam or the Commonwealth of the Northern Mariana Islands pursuant to Title VII of...

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... the Northern Mariana Islands pursuant to Title VII of Public Law 110-229, âConsolidated Natural Resources Act of 2008.â 233.6 Section 233.6 Aliens and Nationality DEPARTMENT OF HOMELAND SECURITY... of the Northern Mariana Islands pursuant to Title VII of Public Law 110-229, “Consolidated...

  15. 8 CFR 233.6 - Aliens entering Guam or the Commonwealth of the Northern Mariana Islands pursuant to Title VII of...

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... the Northern Mariana Islands pursuant to Title VII of Public Law 110-229, âConsolidated Natural Resources Act of 2008.â 233.6 Section 233.6 Aliens and Nationality DEPARTMENT OF HOMELAND SECURITY... of the Northern Mariana Islands pursuant to Title VII of Public Law 110-229, “Consolidated...

  16. Genetic structure, diversity, and interisland dispersal in the endangered Mariana Common Moorhen (Gallinula chloropus guami)

    USGS Publications Warehouse

    Miller, Mark P.; Mullins, Thomas D.; Haig, Susan M.; Takano, Leilani; Garcia, Karla

    2015-01-01

    The Mariana Common Moorhen (Gallinula chloropus guami) is a highly endangered taxon, with fewer than 300 individuals estimated to occur in the wild. The subspecies is believed to have undergone population declines attributable to loss of wetland habitats on its native islands in the Mariana Islands. We analyzed mitochondrial DNA (mtDNA) sequences (control region and ND2 genes) and nuclear microsatellite loci in Mariana Common Moorhens from Guam and Saipan, the two most distal islands inhabited by the subspecies. Our analyses revealed similar nuclear genetic diversity and effective population size estimates on Saipan and Guam. Birds from Guam and Saipan were genetically differentiated (microsatellites: FST = 0.152; control region: FST = 0.736; ND2: FST= 0.390); however, assignment tests revealed the presence of first-generation dispersers from Guam onto Saipan (1 of 27 sampled birds) and from Saipan onto Guam (2 of 28 sampled birds), suggesting the capability for long-distance interpopulation movements within the subspecies. The observed dispersal rate was consistent with long-term estimates of effective numbers of migrants per generation between islands, indicating that movement between islands has been an ongoing process in this system. Despite known population declines, bottleneck tests revealed no signature of historical bottleneck events, suggesting that the magnitude of past population declines may have been comparatively small relative to the severity of declines that can be detected using genetic data.

  17. Detection of Repeating Earthquakes Along the Northern Mariana Shallow Thrust Zone

    NASA Astrophysics Data System (ADS)

    Eimer, M. O.; Wiens, D. A.; Rowe, C. A.

    2014-12-01

    The seismogenic character of the Northern Mariana Thrust Zone is unknown because of the absence of large historical megathrust earthquakes and the lack of appropriate geodetic data. Subduction seismicity in the Northern Mariana forearc shows clustering of events along strike and with depth. To further investigate the seismogenic characteristics of the region, we search for repeating earthquakes along the shallow thrust zone. Using the 2003-2004 Subduction Factory and 2012-2013 Mariana Trench Imaging Experiments, a cross correlation detection scanner is implemented to find repeating earthquakes. Template earthquakes are chosen from seismicity located within clusters along the shallow thrust zone and compared with continuous data from nearby ocean bottom seismometers using both conventional and subspace correlation detection methods. Preliminary results from scanning the 2003-2004 deployment have identified several families of repeating events. Many of these groups consist of repeating events that occur within a short time frame of the template event. In particular, several families have been detected in conjunction with the July 15, 2003 magnitude 5.1 earthquake, indicating detection of aftershock sequences. Repeating events that occur throughout the 2003-2004 yearlong deployment have also been detected, suggesting stable sliding of the plate with small asperities causing these repeating events. Further work may allow delineation of stable sliding regions as well as evaluation of the possible effects of several seamounts which are subducting within the study region.

  18. Fabric and petrological characteristics of serpentinized peridotites from the southern Mariana Trench

    NASA Astrophysics Data System (ADS)

    Michibayashi, K.; Uehara, S.; Ohara, Y.; Ishii, T.

    2011-12-01

    We analysed more than 100 peridotite samples from several sampling points in the southern Mariana Trench-the deepest oceanic trench in the world (10,924±10 m)-to better understand the development of the region. Petrological characteristics indicate the presence of two types of peridotites: those with a backarc origin (Site 1, ~144° E) and those from the forearc (Site 2, ~143.5E and Site 3, ~143E). The peridotites show petrological evidence for intense interaction with magma and/or fluid. This interaction has not influenced the fabric in the samples. On the basis of fabric intensity, the periodotites from Site 1 (backarc origin) and from the Mariana Trough record similar degrees of deformation, whereas the peridotites from Sites 2 and 3 (forearc origin) underwent lower-temperature deformation than did the serpentine seamounts. The spatial distribution of these petrological and fabric characteristics suggests that the mantle beneath the southern Mariana Trench is heterogeneous. Differences in secondary minerals such as serpentine minerals among the samples may reflect the ascent rate, indicating that the peridotites from Site 1 ascended at a higher rate and were serpentinized at a lower temperature (<250 degree C) compared with those from Sites 2 and 3 (>400 degree C).

  19. Earthquake evidence for along-arc extension in the Mariana Islands

    NASA Astrophysics Data System (ADS)

    Heeszel, David S.; Wiens, Douglas A.; Shore, Patrick J.; Shiobara, Hajime; Sugioka, Hiroko

    2008-12-01

    Analysis of data from a deployment of ocean bottom and land seismographs in 2003-2004 detected four swarms of earthquakes in the overriding plate of the Mariana subduction system between the fore-arc and the back-arc spreading center. Two additional shallow swarms were identified by analyzing the teleseismic earthquake catalogs from 1967 to 2003. Focal mechanism solutions for these swarms, determined from regional waveform inversion for the 2003-2004 events or retrieved from the Centroid Moment Tensor catalog for previous years, suggest a complex system of deformation throughout the arc. We observe arc-parallel extension near volcanic cross chains, arc-perpendicular extension along the frontal arc, and arc-parallel compression farther into the back arc near the Mariana Trough. A swarm beneath the middle and eastern summits of the Diamante cross chain may have recorded magmatic activity. Volcanic cross chains showing evidence of adiabatic decompression melting from extensional upwelling are localized at regions of enhanced along-strike extension. The earthquake data are consistent with recent GPS results indicating 12 mm/a of extension between Guam and Agrihan. The along-arc extension may result from either increasing curvature of the Mariana system with time or from deformation induced by oblique subduction in the northernmost and southernmost regions of the arc.

  20. Isolated communities of Epsilonproteobacteria in hydrothermal vent fluids of the Mariana Arc seamounts.

    PubMed

    Huber, Julie A; Cantin, Holly V; Huse, Susan M; Welch, David B Mark; Sogin, Mitchell L; Butterfield, David A

    2010-09-01

    Low-temperature hydrothermal vent fluids represent access points to diverse microbial communities living in oceanic crust. This study examined the distribution, relative abundance, and diversity of Epsilonproteobacteria in 14 low-temperature vent fluids from five volcanically active seamounts of the Mariana Arc using a 454 tag sequencing approach. Most vent fluids were enriched in cell concentrations compared with background seawater, and quantitative PCR results indicated that all fluids were dominated by bacteria. Operational taxonomic unit-based statistical tools applied to 454 data show that all vents from the northern end of the Mariana Arc grouped together, to the exclusion of southern arc seamounts, which were as distinct from one another as they were from northern seamounts. Statistical analysis also showed a significant relationship between seamount and individual vent groupings, suggesting that community membership may be linked to geographical isolation and not geochemical parameters. However, while there may be large-scale geographic differences, distance is not the distinguishing factor in the microbial community composition. At the local scale, most vents host a distinct population of Epsilonproteobacteria, regardless of seamount location. This suggests that there may be barriers to exchange and dispersal for these vent endemic microorganisms at hydrothermal seamounts of the Mariana Arc.

  1. Mollusk collecting and environmental change during the Prehistoric Period in the Mariana Islands

    NASA Astrophysics Data System (ADS)

    Amesbury, J. R.

    2007-12-01

    Archaeological research in the Mariana Islands has revealed changes in mollusk collecting during the Prehistoric Period (approximately 1500 BC to AD 1521). The earliest people at Tumon Bay, Guam and Chalan Piao, Saipan collected mostly bivalves, especially the arc clam Anadara antiquata. After several hundred years, they no longer collected A. antiquata, but collected smaller bivalves instead. By AD 1000, they collected mostly gastropods, primarily the coral reef species Strombus gibberulus gibbosus. One possible explanation is that the people preferred the large arc clam but overharvested it until they were forced to eat the smaller bivalves and then the snails. However, recent evidence in the form of mangrove wood and mangrove pollen supports another explanation, one of non-anthropogenic environmental change. In this case, the relative sea-level decline, which took place in the Marianas within the last 4,000 years, caused the demise of mangrove habitats and of the arc clam at Tumon Bay, Guam and Chalan Piao, Saipan. As mangrove habitats were diminished by sea-level decline, collecting effort shifted to coral reefs, and S. gibberulus gibbosus was harvested throughout the remainder of the Prehistoric Period and into the Historic Period. Southern Guam is the only area in the Marianas in which A. antiquata increased in abundance during the Prehistoric Period. The same types of evidence, mangrove wood and mangrove pollen, indicate that, in contrast to the situation at Tumon Bay and Chalan Piao, mangroves increased in abundance in southern Guam.

  2. Stress assessment and spectral characterization of suspected acid deposition damage in red spruce (Picea Rubens) from Vermont

    NASA Technical Reports Server (NTRS)

    Rock, B. N.; Vogelmann, J. E.

    1985-01-01

    The effects of acid deposition on Picea rubens are studied. The Picea rubens located at Camels Hump Mt., Mt. Ascutney, and Ripton, VT were analyzed using stress level evaluations, in situ spectral data, pressure bomb analysis, and aircraft sensors. Spruce stress per circular plot and percent spruce mortality are calculated. The relation between stress levels and elevation and exposure and weather patterns is examined. It is observed that variations in the reflectance curves of the foliage and branches are related to cellular health, the type of cellular arrangement, and the degree of leaf tissue hydration; the leaf and twig specimens from high stress sites are more reflective in the red portion of the visible and less reflective in the NIR portion of the spectrum. The pressure bomb data reveal that the xylem water tension is higher in specimens from high stress sites. It is noted that remote sensing permits discrimination and mapping of suspected acid deposition damage.

  3. Diets of the Sympatric Pacific Sheath-Tailed Bat (Emballonura semicaudata rotensis) and Mariana Swiftlet (Aerodramus bartschi) on Aguiguan, Mariana Islands

    USGS Publications Warehouse

    Valdez, Ernest W.; Wiles, Gary J.; O'Shea, Thomas J.

    2011-01-01

    The Pacific sheath-tailed bat (Emballonura semicaudata rotensis) and Mariana swiftlet (Aerodramus bartschi) are two rare insectivorous taxa restricted to the southern Mariana Islands in western Micronesia. It is believed that populations of both have dwindled because of impacts to their food resources. However, there is little information on the food habits of A. bartschi and none exists for E. s. rotensis. In an effort to better understand the feeding habits of both, we investigated their diets using guano analysis. Guano was collected from two roosts in caves during a 2-week period in June and July at the onset of the rainy season. Important orders of insects consumed (percentage volume) by bats roosting at one cave included hymenopterans (64%), coleopterans (10%), lepidopterans (8%), isopterans (8%), and psocopterans (5%), whereas those at a second cave included lepidopterans (45%), hymenopterans (41%), coleopterans (10%), and isopterans (5%). Swiftlets, which roosted in only one of the caves, fed mostly on hymenopterans (88%) and hemipterans (6%). Significant differences existed between the two taxa in several insect orders eaten, with E. s. rotensis consuming more lepidopterans and coleopterans and A. bartschi taking more hymenopterans and hemipterans. Within Hymenoptera, bats fed more on ichneumoideans, whereas swiftlets ate more formicid alates and chalicidoideans. This new information on the feeding habits of E. s. rotensis and A. bartschi provides insight on the complexity of their diets during June and July, and serves as baseline information for future studies and management of their habitat.

  4. Spatial Distribution Patterns in the Very Rare and Species-Rich Picea chihuahuana Tree Community (Mexico)

    PubMed Central

    Wehenkel, Christian; Brazão-Protázio, João Marcelo; Carrillo-Parra, Artemio; Martínez-Guerrero, José Hugo; Crecente-Campo, Felipe

    2015-01-01

    The very rare Mexican Picea chihuahuana tree community covers an area of no more than 300 ha in the Sierra Madre Occidental. This special tree community has been the subject of several studies aimed at learning more about the genetic structure and ecology of the species and the potential effects of climate change. The spatial distribution of trees is a result of many ecological processes and can affect the degree of competition between neighbouring trees, tree density, variability in size and distribution, regeneration, survival, growth, mortality, crown formation and the biological diversity within forest communities. Numerous scale-dependent measures have been established in order to describe spatial forest structure. The overall aim of most of these studies has been to obtain data to help design preservation and conservation strategies. In this study, we examined the spatial distribution pattern of trees in the P. chihuahuana tree community in 12 localities, in relation to i) tree stand density, ii) diameter distribution (vertical structure), iii) tree species diversity, iv) geographical latitude and v) tree dominance at a fine scale (in 0.25 ha plots), with the aim of obtaining a better understanding of the complex ecosystem processes and biological diversity. Because of the strongly mixed nature of this tree community, which often produces low population densities of each tree species and random tree fall gaps caused by tree death, we expect aggregated patterns in individual Picea chihuahuana trees and in the P. chihuahuana tree community, repulsive Picea patterns to other tree species and repulsive patterns of young to adult trees. Each location was represented by one plot of 50 x 50 m (0.25 ha) established in the centre of the tree community. The findings demonstrate that the hypothesis of aggregated tree pattern is not applicable to the mean pattern measured by Clark-Evans index, Uniform Angle index and Mean Directional index of the uneven-aged P

  5. Spatial Distribution Patterns in the Very Rare and Species-Rich Picea chihuahuana Tree Community (Mexico).

    PubMed

    Wehenkel, Christian; Brazão-Protázio, João Marcelo; Carrillo-Parra, Artemio; Martínez-Guerrero, José Hugo; Crecente-Campo, Felipe

    2015-01-01

    The very rare Mexican Picea chihuahuana tree community covers an area of no more than 300 ha in the Sierra Madre Occidental. This special tree community has been the subject of several studies aimed at learning more about the genetic structure and ecology of the species and the potential effects of climate change. The spatial distribution of trees is a result of many ecological processes and can affect the degree of competition between neighbouring trees, tree density, variability in size and distribution, regeneration, survival, growth, mortality, crown formation and the biological diversity within forest communities. Numerous scale-dependent measures have been established in order to describe spatial forest structure. The overall aim of most of these studies has been to obtain data to help design preservation and conservation strategies. In this study, we examined the spatial distribution pattern of trees in the P. chihuahuana tree community in 12 localities, in relation to i) tree stand density, ii) diameter distribution (vertical structure), iii) tree species diversity, iv) geographical latitude and v) tree dominance at a fine scale (in 0.25 ha plots), with the aim of obtaining a better understanding of the complex ecosystem processes and biological diversity. Because of the strongly mixed nature of this tree community, which often produces low population densities of each tree species and random tree fall gaps caused by tree death, we expect aggregated patterns in individual Picea chihuahuana trees and in the P. chihuahuana tree community, repulsive Picea patterns to other tree species and repulsive patterns of young to adult trees. Each location was represented by one plot of 50 x 50 m (0.25 ha) established in the centre of the tree community. The findings demonstrate that the hypothesis of aggregated tree pattern is not applicable to the mean pattern measured by Clark-Evans index, Uniform Angle index and Mean Directional index of the uneven-aged P

  6. Multi-Channel Seismic Images of the Mariana Trough: EW0202 Initial Results

    NASA Astrophysics Data System (ADS)

    Taylor, B.; Goodliffe, A. M.; Moore, G. F.; Oakley, A. J.; Fryer, P.

    2002-12-01

    As the first major phase of a US-Japanese collaborative NSF-MARGINS funded project titled "Multi-scale seismic imaging of the Mariana Subduction factory", we collected 5124 km of multi-channel seismic (MCS) data between 14N and 19N across the Mariana subduction-arc-back-arc system. Using the R/V Maurice Ewing, we deployed a 6-km, 480-channel streamer and shot every 50 m using a 20-airgun array (6817 cu in). Coincident gravity, magnetic, and Hydrosweep DS2 data were collected on all 48 lines. The entire MCS dataset, with a common midpoint interval of 6.25 m, was processed through migration during the 30-day cruise. The resulting geophysical transects extend from the Pacific Plate, across the forearc (including several serpentine mud volcanoes), active arc and rifted margin, and back-arc spreading center to the remnant arc. The margins of the back-arc basin are irregular in plan form and contain arc salients that are almost surrounded by back-arc crust. Well-imaged, normal-fault bounded blocks are commonly juxtaposed against back-arc crust with few resolvable internal reflectors. A boundary of this type, with a prominent magnetic anomaly, is imaged on two MCS lines that cross the active arc south of Anatahan volcano at 16.2N. From the position of this boundary, to the east of the arc line, we infer that Anatahan is built on back-arc crust. This likely results in oceanic crust being sandwiched between arc extrusives and cumulates. Other active arc volcanoes may be built on or through rifted former-arc crust. Near 17.5N, 143.5E, an east-dipping low-angle normal fault (approximately 20 degree dip) is imaged beneath the graben at the boundary between the eastern edge of the West Mariana Ridge remnant arc and the oldest crust of the Mariana Trough. North of DSDP Site 451, on the rifted edge of the West Mariana Ridge, there are reflectors at 10-10.5 seconds two-way travel time that may be from the Moho. The back-arc spreading center has an hourglass-shaped inflated segment

  7. Geochemical Composition of Volcanic Rocks from the May 2003 Eruption of Anatahan Volcano, Mariana Islands

    NASA Astrophysics Data System (ADS)

    Wade, J. A.; Plank, T.; Stern, R.; Hilton, D.; Fischer, T. P.; Moore, R.; Trusdell, F.; Sako, M.

    2003-12-01

    The first historical eruption of Anatahan volcano began on May 10, 2003, from the easternmost of the island's two craters. Samples of tephra, scoria, and bombs, collected in May by a MARGINS-supported rapid-response team, were analyzed for 34 trace elements by solution ICP-MS at Boston University and Sr-Nd-Pb isotopic composition at the University of Texas-Dallas. The new eruptive materials can be compared with an extensive suite of pre-existing volcanics (basalts through dacites) from Anatahan sampled by the USGS in 1990 and 1992, and analyzed by XRF and INAA. While most Mariana volcanoes erupt basalts and basaltic andesites, Anatahan is unusual for erupting a wide range of compositions, from basalt to dacite, and thus provides the best opportunity for addressing questions of magma evolution in this classic island arc. The newly erupted scoria and pumice are andesites and dacites that are among the most silicic materials erupted in the northern Mariana islands. The recent eruptives are highly homogeneous; 13 samples vary by only 3-5% relative standard deviation for incompatible trace elements. Isotopic compositions (0.703450 +/- 2 87Sr/86Sr and 18.806 +/- 5 206Pb/204Pb) are within the range of previously measured samples from Anatahan and other volcanic centers in the Marianas. The combined dataset for Anatahan defines virtually a single liquid line of descent. This is consistent with nearly-parallel REE patterns, and small variations in the ratios of the most incompatible trace elements (e.g., Th/Rb varies by <10% over the entire fractionation trend). Low values of Th/La and Th/Zr in Anatahan volcanics provide evidence against partial melting of crustal material as a source of the silicic magmas, as these ratios are highly senstive to apatite- and zircon- saturated crustal melts. Instead, the basalts, andesites and dacites of Anatahan appear to be related predominantly by crystal fractionation with little evidence for assimilation of crustal melts. The new data

  8. The Relationship between Species Diversity and Genetic Structure in the Rare Picea chihuahuana Tree Species Community, Mexico

    PubMed Central

    Simental-Rodríguez, Sergio Leonel; Quiñones-Pérez, Carmen Zulema; Moya, Daniel; Hernández-Tecles, Enrique; López-Sánchez, Carlos Antonio; Wehenkel, Christian

    2014-01-01

    Species diversity and genetic diversity, the most basic elements of biodiversity, have long been treated as separate topics, although populations evolve within a community context. Recent studies on community genetics and ecology have suggested that genetic diversity is not completely independent of species diversity. The Mexican Picea chihuahuana Martínez is an endemic species listed as “Endangered” on the Red List. Forty populations of Chihuahua spruce have been identified. This species is often associated with tree species of eight genera in gallery forests. This rare Picea chihuahuana tree community covers an area no more than 300 ha and has been subject of several studies involving different topics such as ecology, genetic structure and climate change. The overall aim of these studies was to obtain a dataset for developing management tools to help decision makers implement preservation and conservation strategies. However, this unique forest tree community may also represent an excellent subject for helping us to understand the interplay between ecological and evolutionary processes in determining community structure and dynamics. The AFLP technique and species composition data were used together to test the hypothesis that species diversity is related to the adaptive genetic structure of some dominant tree species (Picea chihuahuana, Pinus strobiformis, Pseudotsuga menziesii and Populus tremuloides) of the Picea chihuahuana tree community at fourteen locations. The Hill numbers were used as a diversity measure. The results revealed a significant correlation between tree species diversity and genetic structure in Populus tremuloides. Because the relationship between the two levels of diversity was found to be positive for the putative adaptive AFLP detected, genetic and species structures of the tree community were possibly simultaneously adapted to a combination of ecological or environmental factors. The present findings indicate that interactions

  9. The relationship between species diversity and genetic structure in the rare Picea chihuahuana tree species community, Mexico.

    PubMed

    Simental-Rodríguez, Sergio Leonel; Quiñones-Pérez, Carmen Zulema; Moya, Daniel; Hernández-Tecles, Enrique; López-Sánchez, Carlos Antonio; Wehenkel, Christian

    2014-01-01

    Species diversity and genetic diversity, the most basic elements of biodiversity, have long been treated as separate topics, although populations evolve within a community context. Recent studies on community genetics and ecology have suggested that genetic diversity is not completely independent of species diversity. The Mexican Picea chihuahuana Martínez is an endemic species listed as "Endangered" on the Red List. Forty populations of Chihuahua spruce have been identified. This species is often associated with tree species of eight genera in gallery forests. This rare Picea chihuahuana tree community covers an area no more than 300 ha and has been subject of several studies involving different topics such as ecology, genetic structure and climate change. The overall aim of these studies was to obtain a dataset for developing management tools to help decision makers implement preservation and conservation strategies. However, this unique forest tree community may also represent an excellent subject for helping us to understand the interplay between ecological and evolutionary processes in determining community structure and dynamics. The AFLP technique and species composition data were used together to test the hypothesis that species diversity is related to the adaptive genetic structure of some dominant tree species (Picea chihuahuana, Pinus strobiformis, Pseudotsuga menziesii and Populus tremuloides) of the Picea chihuahuana tree community at fourteen locations. The Hill numbers were used as a diversity measure. The results revealed a significant correlation between tree species diversity and genetic structure in Populus tremuloides. Because the relationship between the two levels of diversity was found to be positive for the putative adaptive AFLP detected, genetic and species structures of the tree community were possibly simultaneously adapted to a combination of ecological or environmental factors. The present findings indicate that interactions between

  10. Two Novel Techniques to Screen Abies Seedlings for Resistance to the Balsam Woolly Adelgid, Adelges piceae

    PubMed Central

    Newton, Leslie; Frampton, John; Monahan, John; Goldfarb, Barry; Hain, Fred

    2011-01-01

    Since its introduction into the Southern Appalachians in the 1950s, the balsam woolly adelgid, Adelges piceae Ratzeburg (Hemiptera: Adelgidae), has devastated native populations of Fraser fir, Abies fraseri (Pursh) Poir. (Pinales: Pinaceae), and has become a major pest in Christmas tree plantations requiring expensive chemical treatments. Adelges piceae—resistant Fraser fir trees would lessen costs for the Christmas tree industry and assist in the restoration of native stands. Resistance screening is an important step in this process. Here, four studies directed toward the development of time— and cost—efficient techniques for screening are reported. In the first study, three methods to artificially infest seedlings of different ages were evaluated in a shade—covered greenhouse. Two—year—old seedlings had much lower infestation levels than 7 year—old seedlings. Placing infested bark at the base of the seedling was less effective than tying infested bark to the seedling or suspending infested bolts above the seedling. Although the two latter techniques resulted in similar densities on the seedlings, they each have positive and negative considerations. Attaching bark to uninfested trees is effective, but very time consuming. The suspended bolt method mimics natural infestation and is more economical than attaching bark, but care must be taken to ensure an even distribution of crawlers falling onto the seedlings. The second study focused on the density and distribution of crawlers falling from suspended bolts onto paper gridded into 7.6 × 7.6 cm cells. Crawler density in a 30 cm band under and to each side of the suspended bolt ranged from 400 to over 3000 crawlers per cell (1 to 55 crawlers per cm2). In the third study, excised branches from 4 year—old A. fraseri and A. vetchii seedlings were artificially infested with A. piceae to determine whether this technique may be useful for early resistance screening. The excised A. fraseri branches supported

  11. Dehydrin accumulation and extreme low-temperature tolerance in Siberian spruce (Picea obovata).

    PubMed

    Kjellsen, Trygve Devold; Yakovlev, Igor A; Fossdal, Carl Gunnar; Strimbeck, G Richard

    2013-12-01

    To investigate the role of dehydrins (DHNs) in extreme low-temperature (LT) tolerance, we sampled needle tissue of Siberian spruce (Picea obovata Ledeb.) from trees growing in an arboretum in Trondheim, Norway from August 2006 to April 2007 and tracked changes in LT tolerance via relative electrolyte leakage. We used western blotting to estimate relative amounts of proteins binding a DHN K-segment antibody, measured relative amounts of nine transcripts for small (<25 kDa) DHNs by quantitative reverse transcription-polymerase chain reaction (PCR) using primers developed for DHN transcripts in a closely related species, Picea abies (L.) Karsten, and isolated and sequenced PCR products for five P. obovata DHNs. Three protein bands of 53, 35 and 33 kDa were detected on western blots of SDS-PAGE-separated protein extracts. The 53-kDa DHN was already present late in the growing season, but accumulated during acclimation, and levels decreased rapidly during deacclimation. The 33- and 35-kDa proteins, identified as Picg5 class DHNs by mass spectrometry, first appeared in detectable amounts late in the acclimation process and remained at detectable levels throughout the period of maximum LT tolerance. Levels of the 53-kDa DHN correlated with two LT tolerance parameters, while results for the 33- and 35-kDa proteins were equivocal due to limited sample size and variation in LT tolerance during the mid-winter period. Three additional bands of 30, 28 and 26 kDa were detected in extracts from needles collected in November 2010 using an immunity-purified antibody. Immunoblotting of two-dimensional gel electrophoresis gels loaded with proteins extracted from October and November samples corroborated the results obtained by SDS-PAGE western blots. One large spot in the 53 kDa range and two trains of spots in the same size range as the 33 and 35 kDa DHNs were detected using the K-segment antibody. Eight of the nine DHN transcripts closely tracked LT tolerance parameters, whereas

  12. Molybdenum mobility and isotopic fractionation during subduction at the Mariana arc

    NASA Astrophysics Data System (ADS)

    Freymuth, Heye; Vils, Flurin; Willbold, Matthias; Taylor, Rex N.; Elliott, Tim

    2015-12-01

    The fate of crustal material recycled into the convecting mantle by plate tectonics is important for understanding the chemical and physical evolution of the planet. Marked isotopic variability of Mo at the Earth's surface offers the promise of providing distinctive signatures of such recycled material. However, characterisation of the behaviour of Mo during subduction is needed to assess the potential of Mo isotope ratios as tracers for global geochemical cycles. Here we present Mo isotope data for input and output components of the archetypical Mariana arc: Mariana arc lavas, sediments from ODP Sites 800, 801 and 802 near the Mariana trench and the altered mafic, oceanic crust (AOC), from ODP Site 801, together with samples of the deeper oceanic crust from ODP Site 1256. We also report new high precision Pb isotope data for the Mariana arc lavas and a dataset of Pb isotope ratios from sediments from ODP Sites 800, 801 and 802. The Mariana arc lavas are enriched in Mo compared to elements of similar incompatibility during upper mantle melting, and have distinct, isotopically heavy Mo (high 98Mo/95Mo) relative to the upper mantle, by up to 0.3 parts per thousand. In contrast, the various subducting sediment lithologies dominantly host isotopically light Mo. Coupled Pb and Mo enrichment in the Mariana arc lavas suggests a common source for these elements and we further use Pb isotopes to identify the origin of the isotopically heavy Mo. We infer that an aqueous fluid component with elevated [Mo], [Pb], high 98Mo/95Mo and unradiogenic Pb is derived from the subducting, mafic oceanic crust. Although the top few hundred metres of the subducting, mafic crust have a high 98Mo/95Mo, as a result of seawater alteration, tightly defined Pb isotope arrays of the Mariana arc lavas extrapolate to a fluid component akin to fresh Pacific mid-ocean ridge basalts. This argues against a flux dominantly derived from the highly altered, uppermost mafic crust or indeed from an Indian

  13. Autotoxicity and allelopathy of 3,4-dihydroxyacetophenone isolated from Picea schrenkiana needles.

    PubMed

    Ruan, Xiao; Li, Zhao-Hui; Wang, Qiang; Pan, Cun-De; Jiang, De-An; Wang, G Geoff

    2011-10-24

    Bioassay-guided fractionation of the diethyl ether fraction of a water extract of Picea schrenkiana needles led to the isolation of the phenolic compound 3,4-dihydroxy- acetophenone (DHAP). The allelopathic effects of DHAP were evaluated under laboratory conditions on P. schrenkiana, rice (Oryza sativa L.), wheat (Triticum aestivum L.), radish (Raphanus sativus L.), lettuce (Latuca sativa L.), cucumber (Cucumis sativus L.) and mung bean (Phaseolus radiatus L.). DHAP significantly inhibited seed germination and seedling growth of P. schrenkiana at concentrations of 2.5 mM and 0.5 mM (p < 0.05). Soil analysis revealed that P. schrenkiana forest soils contained exceptionally high DHAP concentrations (mean = 0.51 ± 0.03 mg/g dry soil), sufficient to inhibit natural P. schrenkiana recruitment. DHAP also exhibited strong allelopathic potential. It significantly inhibited wheat and lettuce seed germination at concentrations of 1 mM and 0.5 mM (p < 0.05). The active compound also completely inhibited root growth of the six test species at high concentrations. Our results suggest a dual role of DHAP, both as an allelochemical and as an autotoxicant. The potential for a single plant needle-leached compound to influence both inter- and intra-specific interactions emphasized the complex effects that plant secondary metabolites might have on plant population and community structure.

  14. Experimental vs. modeled water use in mature Norway spruce (Picea abies) exposed to elevated CO(2).

    PubMed

    Leuzinger, Sebastian; Bader, Martin K-F

    2012-01-01

    Rising levels of atmospheric CO(2) have often been reported to reduce plant water use. Such behavior is also predicted by standard equations relating photosynthesis, stomatal conductance, and atmospheric CO(2) concentration, which form the core of dynamic global vegetation models (DGVMs). Here, we provide first results from a free air CO(2) enrichment (FACE) experiment with naturally growing, mature (35 m) Picea abies (L.) (Norway spruce) and compare them to simulations by the DGVM LPJ-GUESS. We monitored sap flow, stem water deficit, stomatal conductance, leaf water potential, and soil moisture in five 35-40 m tall CO(2)-treated (550 ppm) trees over two seasons. Using LPJ-GUESS, we simulated this experiment using climate data from a nearby weather station. While the model predicted a stable reduction of transpiration of between 9% and 18% (at concentrations of 550-700 ppm atmospheric CO(2)), the combined evidence from various methods characterizing water use in our experimental trees suggest no changes in response to future CO(2) concentrations. The discrepancy between the modeled and the experimental results may be a scaling issue: while dynamic vegetation models correctly predict leaf-level responses, they may not sufficiently account for the processes involved at the canopy and ecosystem scale, which could offset the first-order stomatal response.

  15. Crystal structures of Ophiostoma piceae sterol esterase: structural insights into activation mechanism and product release.

    PubMed

    Gutiérrez-Fernández, Javier; Vaquero, María Eugenia; Prieto, Alicia; Barriuso, Jorge; Martínez, María Jesús; Hermoso, Juan A

    2014-09-01

    Sterol esterases are able to efficiently hydrolyze both sterol esters and triglycerides and to carry out synthesis reactions in the presence of organic solvents. Their high versatility makes them excellent candidates for biotechnological purposes. Sterol esterase from fungus Ophiostoma piceae (OPE) belongs to the family abH03.01 of the Candida rugosa lipase-like proteins. Crystal structures of OPE were solved in this study for the closed and open conformations. Enzyme activation involves a large displacement of the conserved lid, structural rearrangements of loop α16-α17, and formation of a dimer with a large opening. Three PEG molecules are placed in the active site, mimicking chains of the triglyceride substrate, demonstrating the position of the oxyanion hole and the three pockets that accommodate the sn-1, sn-2 and sn-3 fatty acids chains. One of them is an internal tunnel, connecting the active center with the outer surface of the enzyme 30 Å far from the catalytic Ser220. Based on our structural and biochemical results we propose a mechanism by which a great variety of different substrates can be hydrolyzed in OPE paving the way for the construction of new variants to improve the catalytic properties of these enzymes and their biotechnological applications.

  16. Insect herbivory (Choristoneura fumiferana, Tortricidea) underlies tree population structure (Picea glauca, Pinaceae).

    PubMed

    Parent, Geneviève J; Giguère, Isabelle; Germanos, Gaby; Lamara, Mebarek; Bauce, Éric; MacKay, John J

    2017-02-16

    Variation in insect herbivory can lead to population structure in plant hosts as indicated by defence traits. In annual herbaceous, defence traits may vary between geographic areas but evidence of such patterns is lacking for long-lived species. This may result from the variety of selection pressures from herbivores, long distance gene flow, genome properties, and lack of research. We investigated the antagonistic interaction between white spruce (Picea glauca) and spruce budworm (SBW, Choristoneura fumiferana) the most devastating forest insect of eastern North America in common garden experiments. White spruces that are able to resist SBW attack were reported to accumulate the acetophenones piceol and pungenol constitutively in their foliage. We show that levels of these acetophenones and transcripts of the gene responsible for their release is highly heritable and that their accumulation is synchronized with the most devastating stage of SBW. Piceol and pungenol concentrations negatively correlate with rate of development in female SBW and follow a non-random geographic variation pattern that is partially explained by historical damage from SBW and temperature. Our results show that accumulation of acetophenones is an efficient resistance mechanism against SBW in white spruce and that insects can affect population structure of a long-lived plant.

  17. Metabolomic analysis of extreme freezing tolerance in Siberian spruce (Picea obovata).

    PubMed

    Angelcheva, Liudmila; Mishra, Yogesh; Antti, Henrik; Kjellsen, Trygve D; Funk, Christiane; Strimbeck, Richard G; Schröder, Wolfgang P

    2014-11-01

    Siberian spruce (Picea obovata) is one of several boreal conifer species that can survive at extremely low temperatures (ELTs). When fully acclimated, its tissues can survive immersion in liquid nitrogen. Relatively little is known about the biochemical and biophysical strategies of ELT survival. We profiled needle metabolites using gas chromatography coupled with mass spectrometry (GC-MS) to explore the metabolic changes that occur during cold acclimation caused by natural temperature fluctuations. In total, 223 metabolites accumulated and 52 were depleted in fully acclimated needles compared with pre-acclimation needles. The metabolite profiles were found to develop in four distinct phases, which are referred to as pre-acclimation, early acclimation, late acclimation and fully acclimated. Metabolite changes associated with carbohydrate and lipid metabolism were observed, including changes associated with increased raffinose family oligosaccharide synthesis and accumulation, accumulation of sugar acids and sugar alcohols, desaturation of fatty acids, and accumulation of digalactosylglycerol. We also observed the accumulation of protein and nonprotein amino acids and polyamines that may act as compatible solutes or cryoprotectants. These results provide new insight into the mechanisms of freezing tolerance development at the metabolite level and highlight their importance in rapid acclimation to ELT in P. obovata.

  18. Warming delays autumn declines in photosynthetic capacity in a boreal conifer, Norway spruce (Picea abies).

    PubMed

    Stinziano, Joseph R; Hüner, Norman P A; Way, Danielle A

    2015-12-01

    Climate change, via warmer springs and autumns, may lengthen the carbon uptake period of boreal tree species, increasing the potential for carbon sequestration in boreal forests, which could help slow climate change. However, if other seasonal cues such as photoperiod dictate when photosynthetic capacity declines, warmer autumn temperatures may have little effect on when carbon uptake capacity decreases in these species. We investigated whether autumn warming would delay photosynthetic decline in Norway spruce (Picea abies (L.) H. Karst.) by growing seedlings under declining weekly photoperiods and weekly temperatures either at ambient temperature or a warming treatment 4 °C above ambient. Photosynthetic capacity was relatively constant in both treatments when weekly temperatures were >8 °C, but declined rapidly at lower temperatures, leading to a delay in the autumn decline in photosynthetic capacity in the warming treatment. The decline in photosynthetic capacity was not related to changes in leaf nitrogen or chlorophyll concentrations, but was correlated with a decrease in the apparent fraction of leaf nitrogen invested in Rubisco, implicating a shift in nitrogen allocation away from the Calvin cycle at low autumn growing temperatures. Our data suggest that as the climate warms, the period of net carbon uptake will be extended in the autumn for boreal forests dominated by Norway spruce, which could increase total carbon uptake in these forests.

  19. Predicting adaptive phenotypes from multilocus genotypes in Sitka spruce (Picea sitchensis) using random forest.

    PubMed

    Holliday, Jason A; Wang, Tongli; Aitken, Sally

    2012-09-01

    Climate is the primary driver of the distribution of tree species worldwide, and the potential for adaptive evolution will be an important factor determining the response of forests to anthropogenic climate change. Although association mapping has the potential to improve our understanding of the genomic underpinnings of climatically relevant traits, the utility of adaptive polymorphisms uncovered by such studies would be greatly enhanced by the development of integrated models that account for the phenotypic effects of multiple single-nucleotide polymorphisms (SNPs) and their interactions simultaneously. We previously reported the results of association mapping in the widespread conifer Sitka spruce (Picea sitchensis). In the current study we used the recursive partitioning algorithm 'Random Forest' to identify optimized combinations of SNPs to predict adaptive phenotypes. After adjusting for population structure, we were able to explain 37% and 30% of the phenotypic variation, respectively, in two locally adaptive traits--autumn budset timing and cold hardiness. For each trait, the leading five SNPs captured much of the phenotypic variation. To determine the role of epistasis in shaping these phenotypes, we also used a novel approach to quantify the strength and direction of pairwise interactions between SNPs and found such interactions to be common. Our results demonstrate the power of Random Forest to identify subsets of markers that are most important to climatic adaptation, and suggest that interactions among these loci may be widespread.

  20. Molecular events of apical bud formation in white spruce, Picea glauca.

    PubMed

    El Kayal, Walid; Allen, Carmen C G; Ju, Chelsea J-T; Adams, Eri; King-Jones, Susanne; Zaharia, L Irina; Abrams, Suzanne R; Cooke, Janice E K

    2011-03-01

    Bud formation is an adaptive trait that temperate forest trees have acquired to facilitate seasonal synchronization. We have characterized transcriptome-level changes that occur during bud formation of white spruce [Picea glauca (Moench) Voss], a primarily determinate species in which preformed stem units contained within the apical bud constitute most of next season's growth. Microarray analysis identified 4460 differentially expressed sequences in shoot tips during short day-induced bud formation. Cluster analysis revealed distinct temporal patterns of expression, and functional classification of genes in these clusters implied molecular processes that coincide with anatomical changes occurring in the developing bud. Comparing expression profiles in developing buds under long day and short day conditions identified possible photoperiod-responsive genes that may not be essential for bud development. Several genes putatively associated with hormone signalling were identified, and hormone quantification revealed distinct profiles for abscisic acid (ABA), cytokinins, auxin and their metabolites that can be related to morphological changes to the bud. Comparison of gene expression profiles during bud formation in different tissues revealed 108 genes that are differentially expressed only in developing buds and show greater transcript abundance in developing buds than other tissues. These findings provide a temporal roadmap of bud formation in white spruce.

  1. Localization of phenolics in phloem parenchyma cells of Norway spruce (Picea abies).

    PubMed

    Li, Sheng-Hong; Nagy, Nina Elisabeth; Hammerbacher, Almuth; Krokene, Paal; Niu, Xue-Mei; Gershenzon, Jonathan; Schneider, Bernd

    2012-12-21

    Norway spruce (Picea abies) bark contains specialized phloem parenchyma cells that swell and change their contents upon attack by the bark beetle Ips typographus and its microbial associate, the blue stain fungus Ceratocystis polonica. These cells exhibit bright autofluorescence after treatment with standard aldehyde fixatives, and so have been postulated to contain phenolic compounds. Laser microdissection of spruce bark sections combined with cryogenic NMR spectroscopy demonstrated significantly higher concentrations of the stilbene glucoside astringin in phloem parenchyma cells than in adjacent sieve cells. After infection by C. polonica, the flavonoid (+)-catechin also appeared in phloem parenchyma cells and there was a decrease in astringin content compared to cells from uninfected trees. Analysis of whole-bark extracts confirmed the results obtained from the cell extracts and revealed a significant increase in dimeric stilbene glucosides, both astringin and isorhapontin derivatives (piceasides A to H), in fungus-infected versus uninfected bark that might explain the reduction in stilbene monomers. Phloem parenchyma cells thus appear to be a principal site of phenolic accumulation in spruce bark.

  2. Patterns of nucleotide diversity at photoperiod related genes in Norway spruce [Picea abies (L.) Karst].

    PubMed

    Källman, Thomas; De Mita, Stéphane; Larsson, Hanna; Gyllenstrand, Niclas; Heuertz, Myriam; Parducci, Laura; Suyama, Yoshihisa; Lagercrantz, Ulf; Lascoux, Martin

    2014-01-01

    The ability of plants to track seasonal changes is largely dependent on genes assigned to the photoperiod pathway, and variation in those genes is thereby important for adaptation to local day length conditions. Extensive physiological data in several temperate conifer species suggest that populations are adapted to local light conditions, but data on the genes underlying this adaptation are more limited. Here we present nucleotide diversity data from 19 genes putatively involved in photoperiodic response in Norway spruce (Picea abies). Based on similarity to model plants the genes were grouped into three categories according to their presumed position in the photoperiod pathway: photoreceptors, circadian clock genes, and downstream targets. An HKA (Hudson, Kreitman and Aquade) test showed a significant excess of diversity at photoreceptor genes, but no departure from neutrality at circadian genes and downstream targets. Departures from neutrality were also tested with Tajima's D and Fay and Wu's H statistics under three demographic scenarios: the standard neutral model, a population expansion model, and a more complex population split model. Only one gene, the circadian clock gene PaPRR3 with a highly positive Tajima's D value, deviates significantly from all tested demographic scenarios. As the PaPRR3 gene harbours multiple non-synonymous variants it appears as an excellent candidate gene for control of photoperiod response in Norway spruce.

  3. Norway spruce (Picea abies) laccases: characterization of a laccase in a lignin-forming tissue culture.

    PubMed

    Koutaniemi, Sanna; Malmberg, Heli A; Simola, Liisa K; Teeri, Teemu H; Kärkönen, Anna

    2015-04-01

    Secondarily thickened cell walls of water-conducting vessels and tracheids and support-giving sclerenchyma cells contain lignin that makes the cell walls water impermeable and strong. To what extent laccases and peroxidases contribute to lignin biosynthesis in muro is under active evaluation. We performed an in silico study of Norway spruce (Picea abies (L.) Karst.) laccases utilizing available genomic data. As many as 292 laccase encoding sequences (genes, gene fragments, and pseudogenes) were detected in the spruce genome. Out of the 112 genes annotated as laccases, 79 are expressed at some level. We isolated five full-length laccase cDNAs from developing xylem and an extracellular lignin-forming cell culture of spruce. In addition, we purified and biochemically characterized one culture medium laccase from the lignin-forming cell culture. This laccase has an acidic pH optimum (pH 3.8-4.2) for coniferyl alcohol oxidation. It has a high affinity to coniferyl alcohol with an apparent Km value of 3.5 μM; however, the laccase has a lower catalytic efficiency (V(max)/K(m)) for coniferyl alcohol oxidation compared with some purified culture medium peroxidases. The properties are discussed in the context of the information already known about laccases/coniferyl alcohol oxidases of coniferous plants.

  4. Metabolite profiling reveals clear metabolic changes during somatic embryo development of Norway spruce (Picea abies).

    PubMed

    Businge, Edward; Brackmann, Klaus; Moritz, Thomas; Egertsdotter, Ulrika

    2012-02-01

    Progress on industrial-scale propagation of conifers by somatic embryogenesis has been hampered by the differences in developmental capabilities between cell lines, which are limiting the capture of genetic gains from breeding programs. In this study, we investigated the metabolic events occurring during somatic embryo development in Norway spruce to establish a better understanding of the fundamental metabolic events required for somatic embryo development. Three embryogenic cell lines of Norway spruce (Picea abies (L.) Karst) with different developmental capabilities were studied during somatic embryo development from proliferation of proembryogenic masses to mature somatic embryos. The three different cell lines displayed normal, aberrant and blocked somatic embryo development. Metabolite profiles from four development stages in each of the cell lines were obtained using combined gas chromatography-mass spectrometry. Multivariate discriminant analyses of the metabolic data revealed significant metabolites (P  ≤  0.05) for each development stage and transition. The results suggest that endogenous auxin and sugar signaling affects initial stages of somatic embryo development. Furthermore, the results highlight the importance of a timed stress response and the presence of stimulatory metabolites during late stages of embryo development.

  5. Conserved function of core clock proteins in the gymnosperm Norway spruce (Picea abies L. Karst).

    PubMed

    Karlgren, Anna; Gyllenstrand, Niclas; Källman, Thomas; Lagercrantz, Ulf

    2013-01-01

    From studies of the circadian clock in the plant model species Arabidopsis (Arabidopsis thaliana), a number of important properties and components have emerged. These include the genes CIRCADIAN CLOCK ASSOCIATED 1 (CCA1), GIGANTEA (GI), ZEITLUPE (ZTL) and TIMING OF CAB EXPRESSION 1 (TOC1 also known as PSEUDO-RESPONSE REGULATOR 1 (PRR1)) that via gene expression feedback loops participate in the circadian clock. Here, we present results from ectopic expression of four Norway spruce (Picea abies) putative homologs (PaCCA1, PaGI, PaZTL and PaPRR1) in Arabidopsis, their flowering time, circadian period length, red light response phenotypes and their effect on endogenous clock genes were assessed. For PaCCA1-ox and PaZTL-ox the results were consistent with Arabidopsis lines overexpressing the corresponding Arabidopsis genes. For PaGI consistent results were obtained when expressed in the gi2 mutant, while PaGI and PaPRR1 expressed in wild type did not display the expected phenotypes. These results suggest that protein function of PaCCA1, PaGI and PaZTL are at least partly conserved compared to Arabidopsis homologs, however further studies are needed to reveal the protein function of PaPRR1. Our data suggest that components of the three-loop network typical of the circadian clock in angiosperms were present before the split of gymnosperms and angiosperms.

  6. Transcriptional responses of Norway spruce (Picea abies) inner sapwood against Heterobasidion parviporum.

    PubMed

    Oliva, J; Rommel, S; Fossdal, C G; Hietala, A M; Nemesio-Gorriz, M; Solheim, H; Elfstrand, M

    2015-09-01

    The white-rot fungus Heterobasidion parviporum Niemelä & Korhonen establishes a necrotrophic interaction with Norway spruce (Picea abies (L.) H.Karst.) causing root and butt rot and growth losses in living trees. The interaction occurs first with the bark and the outer sapwood, as the pathogen enters the tree via wounds or root-to-root contacts. Later, when the fungus reaches the heartwood, it spreads therein creating a decay column, and the interaction mainly occurs in the inner sapwood where the tree creates a reaction zone. While bark and outer sapwood interactions are well studied, little is known about the nature of the transcriptional responses leading to the creation of a reaction zone. In this study, we sampled bark and sapwood both proximal and distal to the reaction zone in artificially inoculated and naturally infected trees. We quantified gene expression levels of candidate genes in secondary metabolite, hormone biosynthesis and signalling pathways using quantitative polymerase chain reaction. An up-regulation of mainly the phenylpropanoid pathway and jasmonic acid biosynthesis was found at the inoculation site, when inoculations were compared with wounding. We found that transcriptional responses in inner sapwood were similar to those reported upon infection through the bark. Our data suggest that the defence mechanism is induced due to direct fungal contact irrespective of the tissue type. Understanding the nature of these interactions is important when considering tree breeding-based resistance strategies to reduce the spread of the pathogen between and within trees.

  7. The hydraulic conductivity of the xylem in conifer needles (Picea abies and Pinus mugo).

    PubMed

    Charra-Vaskou, Katline; Mayr, Stefan

    2011-08-01

    Main resistances of the plant water transport system are situated in leaves. In contrast to angiosperm leaves, knowledge of conifer needle hydraulics and of the partitioning of resistances within needles is poor. A new technique was developed which enabled flow-meter measurements through needles embedded in paraffin and thus quantification of the specific hydraulic conductivity (K(s)) of the needle xylem. In Picea abies, xylem K(s) of needle and axes as well as in needles of different age were compared. In Pinus mugo, resistance partitioning within needles was estimated by measurements of xylem K(s) and leaf conductance (K(leaf), measured via 'rehydration kinetics'). Mean K(s) in P. abies needles was 3.5×10(-4) m(2) s(-1) MPa(-1) with a decrease in older needles, and over all similar to K(s) of corresponding axes xylem. In needles of P. mugo, K(s) was 0.9×10(-4) m(2) s(-1) MPa(-1), and 24% of total needle resistance was situated in the xylem. The results indicate species-specific differences in the hydraulic efficiency of conifer needle xylem. The vascular section of the water transport system is a minor but relevant resistance in needles.

  8. Resonance wood [Picea abies (L.) Karst.]--evaluation and prediction of violin makers' quality-grading.

    PubMed

    Buksnowitz, Christoph; Teischinger, Alfred; Müller, Ulrich; Pahler, Andreas; Evans, Robert

    2007-04-01

    The definition of quality in the field of resonance wood for musical instrument making has attracted considerable interest over decades but has remained incomplete. The current work compares the traditional knowledge and practical experience of violin makers with a material-science approach to objectively characterize the properties of resonance wood. Norway spruce [Picea abies (L.) Karst.] has earned a very high reputation for the construction of resonance tops of stringed instruments and resonance boards of keyboard instruments, and was therefore chosen as the focus of the investigation. The samples were obtained from numerous renowned resonance wood regions in the European Alps and cover the whole range of available qualities. A set of acoustical, anatomical, mechanical and optical material properties was measured on each sample. These measurements were compared with subjective quality grading by violin makers, who estimated the acoustical, optical and overall suitability for violin making. Multiple linear regression models were applied to evaluate the predictability of the subjective grading using the measured material characteristics as predictors. The results show that luthiers are able to estimate wood quality related to visible features, but predictions of mechanical and acoustical properties proved to be very poor.

  9. Comparison of phloem and xylem hydraulic architecture in Picea abies stems.

    PubMed

    Jyske, Tuula; Hölttä, Teemu

    2015-01-01

    The hydraulic properties of xylem and phloem differ but the magnitude and functional consequences of the differences are not well understood. Phloem and xylem functional areas, hydraulic conduit diameters and conduit frequency along the stems of Picea abies trees were measured and expressed as allometric functions of stem diameter and distance from stem apex. Conductivities of phloem and xylem were estimated from these scaling relations. Compared with xylem, phloem conduits were smaller and occupied a slightly larger fraction of conducting tissue area. Ten times more xylem than phloem was annually produced along the stem. Scaling of the conduit diameters and cross-sectional areas with stem diameter were very similar in phloem and xylem. Phloem and xylem conduits scaled also similarly with distance from stem apex; widening downwards from the tree top, and reaching a plateau near the base of the living crown. Phloem conductivity was estimated to scale similarly to the conductivity of the outermost xylem ring, with the ratio of phloem to xylem conductivity being c. 2%. However, xylem conductivity was estimated to increase more than phloem conductivity with increasing tree dimensions as a result of accumulation of xylem sapwood. Phloem partly compensated for its smaller conducting area and narrower conduits by having a slightly higher conduit frequency.

  10. Diverse ecological roles within fungal communities in decomposing logs of Picea abies.

    PubMed

    Ottosson, Elisabet; Kubartová, Ariana; Edman, Mattias; Jönsson, Mari; Lindhe, Anders; Stenlid, Jan; Dahlberg, Anders

    2015-03-01

    Fungal communities in Norway spruce (Picea abies) logs in two forests in Sweden were investigated by 454-sequence analyses and by examining the ecological roles of the detected taxa. We also investigated the relationship between fruit bodies and mycelia in wood and whether community assembly was affected by how the dead wood was formed. Fungal communities were highly variable in terms of phylogenetic composition and ecological roles: 1910 fungal operational taxonomic units (OTUs) were detected; 21% were identified to species level. In total, 58% of the OTUs were ascomycetes and 31% basidiomycetes. Of the 231 337 reads, 38% were ascomycetes and 60% basidiomycetes. Ecological roles were assigned to 35% of the OTUs, accounting for 62% of the reads. Wood-decaying fungi were the most common group; however, other saprotrophic, mycorrhizal, lichenized, parasitic and endophytic fungi were also common. Fungal communities in logs formed by stem breakage were different to those in logs originating from butt breakage or uprooting. DNA of specific species was detected in logs many years after the last recorded fungal fruiting. Combining taxonomic identification with knowledge of ecological roles may provide valuable insights into properties of fungal communities; however, precise ecological information about many fungal species is still lacking.

  11. Transcript profiling of aquaporins during basidiocarp development in Laccaria bicolor ectomycorrhizal with Picea glauca.

    PubMed

    Xu, Hao; Navarro-Ródenas, Alfonso; Cooke, Janice E K; Zwiazek, Janusz J

    2016-01-01

    Sporocarp formation is part of the reproductive stage in the life cycle of many mycorrhizal macrofungi. Sporocarp formation is accompanied by a transcriptomic switch and profound changes in regulation of the gene families that play crucial roles in the sporocarp initiation and maturation. Since sporocarp growth requires efficient water delivery, in the present study, we investigated changes in transcript abundance of six fungal aquaporin genes that could be cloned from the ectomycorrhizal fungus Laccaria bicolor strain UAMH8232, during the initiation and development of its basidiocarp. Aquaporins are intrinsic membrane proteins facilitating the transmembrane transport of water and other small neutral molecules. In controlled-environment experiments, we induced basidiocarp formation in L. bicolor, which formed ectomycorrhizal associations with white spruce (Picea glauca) seedlings. We profiled transcript abundance corresponding to six fungal aquaporin genes at six different developmental stages of basidiocarp growth and development. We also compared physiological parameters of non-inoculated to mycorrhizal seedlings with and without the presence of basidiocarps. Two L. bicolor aquaporins--JQ585592, a functional channel for CO2, NO and H2O2, and JQ585595, a functional water channel--showed the greatest degree of upregulation during development of the basidiocarp. Our findings point to the importance of aquaporin-mediated transmembrane water and CO2 transport during distinct stages of basidiocarp development.

  12. Terpene chemodiversity of relict conifers Picea omorika, Pinus heldreichii, and Pinus peuce, endemic to Balkan.

    PubMed

    Nikolić, Biljana; Ristić, Mihailo; Tešević, Vele; Marin, Petar D; Bojović, Srdjan

    2011-12-01

    Terpenes are often used as ecological and chemotaxonomic markers of plant species, as well as for estimation of geographic variability. Essential oils of relic and Balkan endemic/subendemic conifers, Picea omorika, Pinus heldreichii, and P. peuce, in central part of Balkan Peninsula (Serbia and Montenegro), on the level of terpene classes and common terpene compounds were investigated. In finding terpene combinations, which could show the best diversity between species and their natural populations, several statistical methods were applied. Apart from the content of different terpene classes (P. omorika has the most abundant O-containing monoterpenes and sesquiterpenes; P. heldreichii and P. peuce have the largest abundance of sesquiterpene and monoterpene hydrocarbons, resp.), the species are clearly separated according to terpene profile with 22 common compounds. But, divergences in their populations were established only in combination of several compounds (specific for each species), and they were found to be the results of geomorphologic, climatic, and genetic factors. We found similarities between investigated species and some taxa from literature with respect to terpene composition, possibly due to hybridization and phylogenetic relations. Obtained results are also important regarding to chemotaxonomy, biogeography, phylogeny, and evolution of these taxa.

  13. Insect herbivory (Choristoneura fumiferana, Tortricidea) underlies tree population structure (Picea glauca, Pinaceae)

    PubMed Central

    Parent, Geneviève J.; Giguère, Isabelle; Germanos, Gaby; Lamara, Mebarek; Bauce, Éric; MacKay, John J.

    2017-01-01

    Variation in insect herbivory can lead to population structure in plant hosts as indicated by defence traits. In annual herbaceous, defence traits may vary between geographic areas but evidence of such patterns is lacking for long-lived species. This may result from the variety of selection pressures from herbivores, long distance gene flow, genome properties, and lack of research. We investigated the antagonistic interaction between white spruce (Picea glauca) and spruce budworm (SBW, Choristoneura fumiferana) the most devastating forest insect of eastern North America in common garden experiments. White spruces that are able to resist SBW attack were reported to accumulate the acetophenones piceol and pungenol constitutively in their foliage. We show that levels of these acetophenones and transcripts of the gene responsible for their release is highly heritable and that their accumulation is synchronized with the most devastating stage of SBW. Piceol and pungenol concentrations negatively correlate with rate of development in female SBW and follow a non-random geographic variation pattern that is partially explained by historical damage from SBW and temperature. Our results show that accumulation of acetophenones is an efficient resistance mechanism against SBW in white spruce and that insects can affect population structure of a long-lived plant. PMID:28205578

  14. Global Lysine Acetylome Analysis of Desiccated Somatic Embryos of Picea asperata

    PubMed Central

    Xia, Yan; Jing, Danlong; Kong, Lisheng; Zhang, Jianwei; OuYang, Fangqun; Zhang, Hanguo; Wang, Junhui; Zhang, Shougong

    2016-01-01

    Partial desiccation treatment (PDT) promotes the germination capacity of conifer somatic embryos. Lysine acetylation (LysAc) is a dynamic and reversible post-translational modification that plays a key role in many biological processes including metabolic pathways and stress response. To investigate the functional impact of LysAc in the response of Picea asperata somatic embryos to PDT, we performed a global lysine acetylome analysis. Here, combining antibody-based affinity enrichment and high-resolution mass spectrometry, we identified and validated 1079 acetylation sites in 556 acetylated proteins from P. asperata somatic embryos during PDT. These data represent a novel large-scale dataset of lysine-acetylated proteins from the conifer family. Intensive bioinformatics analysis of the Gene Ontology of molecular functions demonstrated that lysine-acetylated proteins were mainly associated with binding, catalytic activities, and structural molecular activities. Functional characterization of the acetylated proteins revealed that in the desiccated somatic embryos, LysAc is mainly involved in the response to stress and central metabolism. Accordingly, the majority of these interacting proteins were also highly enriched in ribosome, proteasome, spliceosome, and carbon metabolism clusters. This work provides the most comprehensive profile of LysAc for a coniferous species obtained to date and facilitates the systematic study of the physiological role of LysAc in desiccated somatic embryos of P. asperata. PMID:28066480

  15. Antioxidants and Manganese Deficiency in Needles of Norway Spruce (Picea abies L.) Trees 1

    PubMed Central

    Polle, Andrea; Chakrabarti, Krisanu; Chakrabarti, Sila; Seifert, Friederike; Schramel, Peter; Rennenberg, Heinz

    1992-01-01

    Chlorotic and green needles from Norway spruce (Picea abies L.) trees were sampled in the Calcareous Bavarian Alps in winter. The needles were used for analysis of the mineral and pigment contents, the levels of antioxidants (ascorbate, glutathione), and the activities of protective enzymes (superoxide dismutase, catalase, ascorbate peroxidase, monodehydroascorbate radical reductase, dehydroascorbate reductase, glutathione reductase). In addition, the activities of two respiratory enzymes (glucose-6-phosphate dehydrogenase, NAD-malate dehydrogenase), which might provide the NADPH necessary for functioning of the antioxidative system, were determined. We found that chlorotic needles were severely manganese deficient (3 to 6 micrograms Mn per gram dry weight as compared with up to 190 micrograms Mn per gram dry weight in green needles) but had a similar dry weight to fresh weight ratio, had a similar protein content, and showed no evidence for enhanced lipid peroxidation as compared with green needles. In chlorotic needles, the level of total ascorbate and the activities of superoxide dismutase, monodehydroascorbate radical reductase, NAD-malate dehydrogenase, and glucose-6-phosphate dehydrogenase were significantly increased, whereas the levels of ascorbate peroxidase, dehydroascorbate reductase, glutathione reductase, and glutathione were not affected. The ratio of ascorbate to dehydroascorbate was similar in both green and chlorotic needles. These results suggest that in spruce needles monodehydroascorbate radical reductase is the key enzyme involved in maintaining ascorbate in its reduced state. The reductant necessary for this process may have been supplied at the expense of photosynthate. PMID:16668974

  16. Genetic architecture and genomic patterns of gene flow between hybridizing species of Picea

    PubMed Central

    De La Torre, A; Ingvarsson, P K; Aitken, S N

    2015-01-01

    Hybrid zones provide an opportunity to study the effects of selection and gene flow in natural settings. We employed nuclear microsatellites (single sequence repeat (SSR)) and candidate gene single-nucleotide polymorphism markers (SNPs) to characterize the genetic architecture and patterns of interspecific gene flow in the Picea glauca × P. engelmannii hybrid zone across a broad latitudinal (40–60 degrees) and elevational (350–3500 m) range in western North America. Our results revealed a wide and complex hybrid zone with broad ancestry levels and low interspecific heterozygosity, shaped by asymmetric advanced-generation introgression, and low reproductive barriers between parental species. The clinal variation based on geographic variables, lack of concordance in clines among loci and the width of the hybrid zone points towards the maintenance of species integrity through environmental selection. Congruency between geographic and genomic clines suggests that loci with narrow clines are under strong selection, favoring either one parental species (directional selection) or their hybrids (overdominance) as a result of strong associations with climatic variables such as precipitation as snow and mean annual temperature. Cline movement due to past demographic events (evidenced by allelic richness and heterozygosity shifts from the average cline center) may explain the asymmetry in introgression and predominance of P. engelmannii found in this study. These results provide insights into the genetic architecture and fine-scale patterns of admixture, and identify loci that may be involved in reproductive barriers between the species. PMID:25806545

  17. Physiology and growth of advance Picea rubens and Abies balsamea regeneration following different canopy openings.

    PubMed

    Dumais, Daniel; Prévost, Marcel

    2014-02-01

    We examined the ecophysiology and growth of 0.3-1.3 m tall advance red spruce (Picea rubens Sarg.) and balsam fir (Abies balsamea [L.] Mill.) regeneration during a 5-year period following the application of different harvest types producing three sizes of canopy openings: (i) small gaps (<100 m(2) in area; SMA) created by partial uniform single-tree harvest; (ii) irregular gaps of intermediate size (100-300 m(2); INT) created by group-selection harvest (removal of groups of trees, mainly balsam fir, with uniform partial removal between groups); and (iii) large circular gaps (700 m(2); LAR) created by patch-selection harvest (removal of trees in 30-m diameter circular areas with uniform partial removal between gaps). An unharvested control (CON) was monitored for comparison. At the ecophysiological level, we mainly found differences in light-saturated photosynthesis of red spruce and specific leaf area of balsam fir among treatments. Consequently, we observed good height growth of both species in CON and INT, but fir surpassed spruce in SMA and LAR. Results suggest that intermediate 100-300 m(2) irregular openings create microenvironmental conditions that may promote short-term ecophysiology and growth of red spruce, allowing the species to compete with balsam fir advance regeneration. Finally, results observed for spruce in large 700-m(2) openings confirm its inability to grow as rapidly as fir in comparable open conditions.

  18. Laboratory studies of feeding and oviposition preference, developmental performance, and survival of the predatory beetle, Sasajiscymnus tsugae on diets of the woolly adelgids, Adelges tsugae and Adelges piceae.

    PubMed

    Jetton, Robert M; Monahan, John F; Hain, Fred P

    2011-01-01

    The suitability of the balsam woolly adelgid, Adelges piceae Ratzeburg (Hemiptera: Adelgidae) as an alternate mass rearing host for the adelgid predator, Sasajiscymnus tsugae Sasaji and McClure (Coleoptera: Coccinellidae) was studied in the laboratory. This predator is native to Japan and has been introduced to eastern hemlock, Tsuga canadensis (L.) Carrière (Pinales: Pinaceae), forests throughout the eastern United States for biological control of the hemlock woolly adelgid, Adelges tsugae Annand (Hemiptera: Adelgidae), also of Japanese origin. Feeding, oviposition, immature development, and adult long-term survival of S. tsugae were tested in a series of no choice (single-prey) and paired-choice experiments between the primary host prey, A. tsugae, and the alternate host prey, A. piceae. In paired-choice feeding tests, the predator did not discriminate between eggs of the two adelgid species, but in the no choice tests the predator did eat significantly more eggs of A. piceae than those of A. tsugae. S. tsugae accepted both test prey for oviposition and preferred to lay eggs on adelgid infested versus noninfested host plants. Overall oviposition rates were very low (< 1 egg per predator female) in the oviposition preference tests. Predator immature development rates did not differ between the two test prey, but only 60% of S. tsugae survived egg to adult development when fed A. piceae compared to 86% when fed A. tsugae. S. tsugae adult long-term survival was significantly influenced (positively and negatively) by prey type and the availability of a supplemental food source (diluted honey) when offered aestivating A. tsugae sistens nymphs or ovipositing aestivosistens A. piceae adults, but not when offered ovipositing A. tsugae sistens adults. These results suggest that the development of S. tsugae laboratory colonies reared on a diet consisting only of A. piceae may be possible, and that the biological control potential of the predator might be expanded to

  19. Transport of airborne Picea schrenkiana pollen on the northern slope of Tianshan Mountains (Xinjiang, China) and its implication for paleoenvironmental reconstruction.

    PubMed

    Pan, Yanfang; Yan, Shun; Behling, Hermann; Mu, Guijin

    2013-06-01

    The understanding of airborne pollen transportation is crucial for the reconstruction of the paleoenvironment. Under favorable conditions, a considerable amount of long-distance-transported pollen can be deposited far from its place of origin. In extreme arid regions, in most cases, such situations occur and increase the difficulty to interpret fossil pollen records. In this study, three sets of Cour airborne pollen trap were installed on the northern slope of Tianshan Mountains to collect airborne Picea schrenkiana (spruce) pollen grains from July 2001 to July 2006. The results indicate that Picea pollen disperses extensively and transports widely in the lower atmosphere far away from spruce forest. The airborne Picea pollen dispersal period is mainly concentrated between mid-May and July. In desert area, weekly Picea pollen began to increase and peaked suddenly in concentration. Also, annual pollen indices do not decline even when the distance increased was probably related to the strong wind may pick up the deposited pollen grains from the topsoil into the air stream, leading to an increase of pollen concentration in the air that is irrelevant to the normal and natural course of pollen transport and deposition. This, in turn, may lead to erroneous interpretations of the pollen data in the arid region. This study provided insight into the shift in the Picea pollen season regarding climate change in arid areas. It is recorded that the pollen pollination period starts earlier and the duration became longer. The results also showed that the temperature of May and June was positively correlated with the Picea pollen production. Furthermore, the transport of airborne Picea pollen data is useful for interpreting fossil pollen records from extreme arid regions.

  20. Structure and composition of the Southern Mariana Forearc: new observations and samples from Shinkai 6500 dive studies in 2010

    NASA Astrophysics Data System (ADS)

    Ohara, Y.; Reagan, M. K.; Ishizuka, O.; Stern, R. J.

    2010-12-01

    The 3000-km long Izu-Bonin-Mariana (IBM) Arc system is an outstanding example of an intraoceanic convergent plate margin, and has become the particular focus of Japanese and US efforts to understand the operation of the “Subduction Factory”. In 2006 and 2008, twelve DSV Shinkai 6500 dives (973-977 and 1091-1097) were performed during YK06-12 and YK08-08 Leg 2 cruises along the landward slope of the southern Mariana Trench. The goal was to sample the remaining early arc crust associated with subduction initiation in the IBM system and upper mantle exposed in the forearc in order to gain a clearer understanding of the structure and evolution of Mariana forearc crust and upper mantle. The fruitful results include the recovery of the entire suite of rocks associated with what could be termed a “supra-subduction zone ophiolite” that formed during subduction initiation. An important discovery is that MORB-like tholeiitic basalts crop out over large areas. These “fore-arc basalts” (FAB) underlie boninites and overlie diabasic and gabbroic rocks. Potential origins include eruption at a spreading center before subduction began or eruption during near-trench spreading after subduction began (Reagan et al., 2010, G3). Another important discovery is a region of active forearc rifting at the southern end of the Mariana arc, named SE Mariana Forearc Rift (SEMFR). The SEMFR was firstly mapped with HMR-1 sonar (Martinez et al., 2000, JGR). Two dives at SEMFR recovered less-depleted backarc related peridotites (at Dive 973; Michibayashi et al., 2009, G3), and fresh basalts and basaltic andesites with petrographic characteristics like backarc basin lavas (at Dive 1096; see Ribeiro et al., AGU FM 2010). Although our previous studies have produced a number of important new observations about the geology of the southern Mariana forearc, our understanding of the region is still primitive. We will be conducting another cruise (YK10-12) during late September, 2010 to tackle

  1. Results of ODP Leg 125 drilling in the Mariana/Izu-Bonin forearcs

    SciTech Connect

    Fryer, P. ); Pearce, J.A. ); Stokking, L. )

    1990-06-01

    ODP Leg 125 drilled a total of nine sites in the Mariana and Izu-Bonin forearcs, the regions between the active volcanic arc and the trench axis. Six sites were located on or adjacent to serpentine seamounts, four of these (Sites 778-781) on Conical Seamount in the Mariana forearc, and two others (783 and 784) on the Torishima Forearc Seamount in the Izu-Bonin forearc. The remaining sites (Sites 782, 785, and 786) were drilled into volcanic sequences along the eastern edge of the Izu-Bonin forearc basin. The principal results of the drilling were to achieve (1) the recovery of the first evidence for Pliocene or younger magmatic activity in an extant intraoceanic forearc terrain; (2) the first deep penetration of the Eocene basement of the Izu-Bonin outer-arc high to recover 650 m of boninite flows and hyaloclastite, andesite-dacite flows, breccias, sills, and dikes; (3) the confirmation that some forearc serpentine seamounts can form by flows of clast-bearing serpentine mud from a central conduit, as do mud volcanoes; (4) the discovery of mafic clasts within the serpentine mud flows that have both IAT and MORB affinities, that are metamorphosed in the low to moderate temperature/pressure regimes; (5) the confirmation of high-pH, low-chlorinity fluids at shallow levels near the summit of the seamount which probably originated beneath the forearc and are subduction-related; (6) the recovery of complex hydrocarbon-rich gases also of probable subduction related origin within the Mariana serpentine seamount; and (7) the identification of numerous ash layers within the Izu-Bonin forearc basin that indicate peaks of volcanic activity in the Eocene-Oligocene and from the late Miocene to the Holocene.

  2. Looking for Larvae Above an Erupting Submarine Volcano, NW Rota-1, Mariana Arc

    NASA Astrophysics Data System (ADS)

    Hanson, M.; Beaulieu, S.; Tunnicliffe, V.; Chadwick, W.; Breuer, E. R.

    2015-12-01

    In 2009 the first marine protected areas for deep-sea hydrothermal vents in U.S. waters were established as part of the Volcanic Unit of the Marianas Trench Marine National Monument. In this region, hydrothermal vents are located along the Mariana Arc and back-arc spreading center. In particular hydrothermal vents are located near the summit of NW Rota-1, an active submarine volcano on the Mariana Arc which was erupting between 2003 through 2010 and ceased as of 2014. In late 2009, NW Rota-1 experienced a massive landslide decimating the habitat on the southern side of the volcano. This presented an enormous natural disturbance to the community. This project looked at zooplankton tow samples taken from the water column above NW Rota-1 in 2010, searching specifically for larvae which have the potential to recolonize the sea floor after such a major disturbance. We focused on samples for which profiles with a MAPR sensor indicated hydrothermal plumes in the water column. Samples were sorted in entirety into coarse taxa, and then larvae were removed for DNA barcoding. Overall zooplankton composition was dominated by copepods, ostracods, and chaetognaths, the majority of which are pelagic organisms. Comparatively few larvae of benthic invertebrates were found, but shrimp, gastropod, barnacle, and polychaete larvae did appear in low numbers in the samples. Species-level identification obtained via genetic barcoding will allow for these larvae to be matched to species known to inhabit the benthic communities at NW Rota-1. Identified larvae will give insight into the organisms which can re-colonize the seafloor vent communities after a disturbance such as the 2009 landslide. Communities at hydrothermal vents at other submarine volcanoes in the Monument also can act as sources for these planktonic, recolonizing larvae. As the microinvertebrate biodiversity in the Monument has yet to be fully characterized, our project also provides an opportunity to better describe both

  3. Diets of the sympatric pacific sheath-tailed bat (Emballonura semicaudata rotensis) and Mariana Swiftlet (Aerodramus bartscht) on Aguiguan, Mariana Islands

    USGS Publications Warehouse

    Valdez, E.W.; Wiles, G.J.; O'Shea, T.J.

    2011-01-01

    The Pacific sheath-tailed bat (Emballonura semicaudata rotensis) and Mariana swiftlet (Aerodramus bartschi) are two rare insectivorous taxa restricted to the southern Mariana Islands in western Micronesia. It is believed that populations of both have dwindled because of impacts to their food resources. However, there is little information on the food habits of A. bartschi and none exists for E. s. rotensis. In an effort to better understand the feeding habits of both, we investigated their diets using guano analysis. Guano was collected from two roosts in caves during a 2-week period in June and July at the onset of the rainy season. Important orders of insects consumed (percentage volume) by bats roosting at one cave included hymenopterans (64%), coleopterans (10%), lepidopterans (8%), isopterans (8%), and psocopterans (5%), whereas those at a second cave included lepidopterans (45%), hymenopterans (41%), coleopterans (10%), and isopterans (5%). Swiftlets, which roosted in only one of the caves, fed mostly on hymenopterans (88%) and hemipterans (6%). Significant differences existed between the two taxa in several insect orders eaten, with E. s. rotensis consuming more lepidopterans and coleopterans and A. bartschi taking more hymenopterans and hemipterans. Within Hymenoptera, bats fed more on ichneumoideans, whereas swiftlets ate more formicid alates and chalicidoideans. This new information on the feeding habits of E. s. rotensis and A. bartschi provides insight on the complexity of their diets during June and July, and serves as baseline information for future studies and management of their habitat. ?? 2011 by University of Hawai'i Press All rights reserved.

  4. Genome-wide analysis reveals diverged patterns of codon bias, gene expression, and rates of sequence evolution in picea gene families.

    PubMed

    De La Torre, Amanda R; Lin, Yao-Cheng; Van de Peer, Yves; Ingvarsson, Pär K

    2015-03-05

    The recent sequencing of several gymnosperm genomes has greatly facilitated studying the evolution of their genes and gene families. In this study, we examine the evidence for expression-mediated selection in the first two fully sequenced representatives of the gymnosperm plant clade (Picea abies and Picea glauca). We use genome-wide estimates of gene expression (>50,000 expressed genes) to study the relationship between gene expression, codon bias, rates of sequence divergence, protein length, and gene duplication. We found that gene expression is correlated with rates of sequence divergence and codon bias, suggesting that natural selection is acting on Picea protein-coding genes for translational efficiency. Gene expression, rates of sequence divergence, and codon bias are correlated with the size of gene families, with large multicopy gene families having, on average, a lower expression level and breadth, lower codon bias, and higher rates of sequence divergence than single-copy gene families. Tissue-specific patterns of gene expression were more common in large gene families with large gene expression divergence than in single-copy families. Recent family expansions combined with large gene expression variation in paralogs and increased rates of sequence evolution suggest that some Picea gene families are rapidly evolving to cope with biotic and abiotic stress. Our study highlights the importance of gene expression and natural selection in shaping the evolution of protein-coding genes in Picea species, and sets the ground for further studies investigating the evolution of individual gene families in gymnosperms.

  5. Flexural bending of the oceanic plates near the Mariana, Japan, and Philippines trenches

    NASA Astrophysics Data System (ADS)

    Tang, M.; Lin, J.; Zhang, F.

    2013-12-01

    We conducted a detailed analysis of flexural bending of oceanic plates near the Mariana, Japan, and Philippines trenches to better understand the similarities and differences among these major subduction systems in the western Pacific Ocean. For each of the systems, we first obtained a 3-D deformation surface of the subducting plate by removing from the seafloor bathymetry the estimated topographic effects of sediment thickness, seamounts, and age-related thermal subsidence. We then calculated theoretical models of plate deformation along a series of trench-perpendicular profiles and inverted for the vertical force (Vo) and bending moment (Mo) at the trench axis, as well as variations in the elastic plate thickness (Te) that can best explain the observed plate deformation. From analysis of profiles across all trenches, we found that Te is reduced significantly from a value seaward of the outer rise (TeMax) to a value near the trench (TeMin), with the transition at distance Xr from the trench axis. Results of analysis reveal that the Mariana trench has the greatest amplitude of flexural bending (i.e., the greatest trench depth) in the range of 1.39 - 5.67 km and an average of 2.91 km, comparing to the Japan trench (range of 1.0 - 4.08 km, average of 2.59 km) and the Philippines trench (range of 0.48 - 4.04 km, average of 2.41 km). In contrast, the Philippines trench has the relatively narrow trench width (Xr range of 36 - 107 km, average of 68 km), in comparison to the Japan trench (Xr range of 47 - 122 km, average of 83 km) and the Mariana trench (Xr range of 60 - 125 km, average of 92 km). The best-fitting models reveal that for the Mariana trench, the effective elastic thickness is reduced significantly from a value seaward of the outer rise (TeMax = 45 - 55 km) to a value trench-ward of the outer rise region (TeMin = 19 - 40 km), with a corresponding reduction in Te in the range of 20 - 60%. In comparison, for the Japan trench, TeMax = 35 - 55 km, TeMin = 14

  6. A complex baleen whale call recorded in the Mariana Trench Marine National Monument.

    PubMed

    Nieukirk, Sharon L; Fregosi, Selene; Mellinger, David K; Klinck, Holger

    2016-09-01

    In fall 2014 and spring 2015, passive acoustic data were collected via autonomous gliders east of Guam in an area that included the Mariana Trench Marine National Monument. A short (2-4 s), complex sound was recorded that features a ∼38 Hz moan with both harmonics and amplitude modulation, followed by broad-frequency metallic-sounding sweeps up to 7.5 kHz. This sound was recorded regularly during both fall and spring surveys. Aurally, the sound is quite unusual and most resembles the minke whale "Star Wars" call. It is likely this sound is biological and produced by a baleen whale.

  7. Archaeological and Historical Reconnaissance Survey of the Ugum River Valley, Guam, Mariana Islands,

    DTIC Science & Technology

    1978-08-01

    important food plant in Micro- neisa; and betel nut ( Areca catechu), chewed for its stimulant effect. Although feral yams (Dioscorea sp.) were not found...ANTHROPOLOGY T S DYE ET AL. AUG 78 UNCLASSIFIED DACMdB4-77-C-eei9 F/G 5/6 M monsoon 0 E-IiE*uuuusuimuu-:u r2 IIII= *L. m 1132 0- L .13 j.6I1 __ 111 , ____ U...MARIANA ISLANDS by Thomas S. Dye , Bernice P. Bishop Museum and Samuel T. Price 𔃽 and ,John L. Craib, Pacific Studies Institute Prepared for: U.S. Army

  8. Migrating shoshonitic magmatism tracks Izu-Bonin-Mariana intra-oceanic arc rift propagation

    NASA Astrophysics Data System (ADS)

    Ishizuka, O.; Yuasa, M.; Tamura, Y.; Shukuno, H.; Stern, R. J.; Naka, J.; Joshima, M.; Taylor, R. N.

    2010-05-01

    The southernmost Izu-Bonin arc and northernmost Mariana arc are characterized by K-rich and shoshonitic lavas, referred to as the alkalic volcano province (AVP). These compositions are unusual for intra-oceanic arcs and the interpretation of the AVP is controversial. Rifting to form the Mariana Trough back-arc basin occurs just south of the AVP although back-arc seafloor spreading has not begun. Here we report the results of dredge sampling of the West Mariana Ridge (WMR) in the region of rift propagation; this recovered exclusively medium K to shoshonitic basalts that show clear arc-like geochemical signatures. Ar-Ar ages of WMR shoshonitics systematically young northward. Age of c. 6 Ma was obtained at 21.5°N, c. 3 Ma at 23-23.5°N, and zero-age shoshonites occur on Io-to Island (formerly Iwo Jima) at 24.8°N. Shoshonitic magmatism migrated northward at 4.3 cm/year, in advance of northward-propagating Mariana Trough rifting. This implies that AVP shoshonitic magmatism manifests processes and sources that are uniquely associated with earliest back-arc basin rifting. High-precision Pb isotopic analyses reveal that WMR lavas form a single trend between 2 components, one with lower 206Pb/ 204Pb and high Δ7/4 (arc-like), and another with high 206Pb/ 204Pb as well as low Δ 7/4 and 8/4 (HIMU-like). These components could correspond respectively to subducted pelagic sediment and subducted seamounts and volcaniclastics with HIMU isotopic signature. These slab-derived components alone, however, cannot fully explain chemical characteristics of WMR shoshonitic lavas. These lavas require a component with high Δ7/4 and high Ce/Pb, which is not likely to be either pelagic sediment or seamount volcanics. This component is only expressed when rifting begins, suggesting that it resides in enriched lithosphere or uppermost asthenosphere, which is easily melted due to decompression caused by rifting, when the lithosphere is first ruptured. This component might be linked to slow

  9. Larval abundance and dispersal at deep-sea hydrothermal vents in the southern Mariana Trough

    NASA Astrophysics Data System (ADS)

    Beaulieu, S. E.; Watanabe, H.; Mills, S. W.; Pradillon, F.; Kojima, S.; Mullineaux, L. S.

    2010-12-01

    Since the discovery of deep-sea hydrothermal vents over thirty years ago, scientists have been perplexed by the question: “How are the faunal populations established and maintained at these very discrete and often ephemeral habitats?” For the animals that are sessile or have limited mobility as adults, dispersal to these habitats occurs early in the life cycle, as planktonic larvae in the water column. Due to the difficulties in sampling deep-sea larvae, including low abundances (dilute concentrations), we have very few quantitative estimates of larval dispersal between or larval supply to hydrothermal vents. Here, we will present results of an international, collaborative effort to study larval abundance and dispersal at vents near the back-arc spreading center in the southern Mariana Trough. On R/V Yokosuka cruise YK10-11 in September 2010, we will deploy large-volume plankton pumps at approximately 3000-m depth at Snail (also called South Backarc), Archaean, and Pika, three of the Vents (Volcanic) Unit sites in the U.S. Marianas Trench Marine National Monument. These vents are being studied as part of the Japanese multi-disciplinary program TAIGA (Trans-crustal Advection and In-situ biogeochemical proceses of Global sub-sea floor Aquifer). These will be the first collections of vent larvae in the Mariana back-arc, and we will compare the abundances and diversity of larvae to similar work conducted at the Ridge 2000 East Pacific Rise Integrated Studies Site. We will deploy a current meter near the Snail site (on-axis) for preliminary estimates of passive larval transport on the time scale of the cruise. The three study sites are situated in a line perpendicular to the back-arc spreading axis, and thus may be interesting in terms of local dispersal processes. Perhaps more interesting, however, is that the back-arc vents that we will visit are as close as 25 km to known vents on the arc, yet 600 km south of the other known vents in the back-arc. These two

  10. Diffuse Crustal Accretion at the Southern Terminus of the Malaguana-Gadao Ridge, Mariana Trough

    NASA Astrophysics Data System (ADS)

    Sleeper, J. D.; Martinez, F.; Fryer, P. B.

    2014-12-01

    The mode of extension and crustal accretion in backarc basins is strongly affected by proximity to the arc volcanic front. The factor that likely has the strongest control on these processes is mantle water content. At Mid-Ocean Ridges, the small amount of water in the mantle is efficiently extracted into the melt, dehydrating the residual material and increasing the viscosity and strength of the lithosphere. This may aid in focusing melt generated over a broad (~200+ km wide) zone in the mantle toward a narrow zone of crustal accretion ~1-2 km wide. In the near-arc setting, the continuous flux of water into the mantle wedge should oppose lithospheric dehydration and inhibit strengthening of the lithosphere, which may allow deformation, volcanism, and crustal accretion to occur over a broad area instead of along a narrow axis. A possible example of this process can be observed at the southern terminus of the Malaguana-Gadao Ridge, a backarc spreading center in the Southern Mariana Trough, at the southern end of the Izu-Bonin-Mariana convergent margin. The spreading axis, which forms an axial high in this area, abruptly terminates at 143˚20'E, 12˚37'N and is replaced by a broad zone of active volcanism and tectonism characterized by short volcanic ridges, volcanic cones, and low-relief grabens. This study uses deep-towed and ship multibeam sonar, gravity, and magnetics data collected during an early 2012 cruise on R/V Thomas G. Thompson (TN273) along with available geophysical and geochemical data in the Southern Mariana Trough to gain insight into the nature of the diffuse crustal accretion process. Evidence of a similar transition from organized to "disorganized" spreading can also be observed at Valu Fa Ridge in the southern Lau basin and other backarc spreading centers. This suggests that this process is not unique to the Southern Mariana Trough, and may be an important mode of crustal accretion in a variety of backarc settings where there is extension in

  11. Upper mantle electrical resistivity structure beneath the central Mariana subduction system

    NASA Astrophysics Data System (ADS)

    Matsuno, Tetsuo; Seama, Nobukazu; Evans, Rob L.; Chave, Alan D.; Baba, Kiyoshi; White, Antony; Goto, Tada-Nori; Heinson, Graham; Boren, Goran; Yoneda, Asami; Utada, Hisashi

    2010-09-01

    This paper reports on a magnetotelluric (MT) survey across the central Mariana subduction system, providing a comprehensive electrical resistivity image of the upper mantle to address issues of mantle dynamics in the mantle wedge and beneath the slow back-arc spreading ridge. After calculation of MT response functions and their correction for topographic distortion, two-dimensional electrical resistivity structures were generated using an inversion algorithm with a smoothness constraint and with additional restrictions imposed by the subducting slab. The resultant isotropic electrical resistivity structure contains several key features. There is an uppermost resistive layer with a thickness of up to 150 km beneath the Pacific Ocean Basin, 80-100 km beneath the Mariana Trough, and 60 km beneath the Parece Vela Basin along with a conductive mantle beneath the resistive layer. A resistive region down to 60 km depth and a conductive region at greater depth are inferred beneath the volcanic arc in the mantle wedge. There is no evidence for a conductive feature beneath the back-arc spreading center. Sensitivity tests were applied to these features through inversion of synthetic data. The uppermost resistive layer is the cool, dry residual from the plate accretion process. Its thickness beneath the Pacific Ocean Basin is controlled mainly by temperature, whereas the roughly constant thickness beneath the Mariana Trough and beneath the Parece Vela Basin regardless of seafloor age is controlled by composition. The conductive mantle beneath the uppermost resistive layer requires hydration of olivine and/or melting of the mantle. The resistive region beneath the volcanic arc down to 60 km suggests that fluids such as melt or free water are not well connected or are highly three-dimensional and of limited size. In contrast, the conductive region beneath the volcanic arc below 60 km depth reflects melting and hydration driven by water release from the subducting slab. The

  12. Status and trends of the land bird avifauna on Tinian and Aguiguan, Mariana Islands.

    USGS Publications Warehouse

    Camp, Richard J.; Pratt, Thane K.; Amidon, Fred; Marshall, Ann P.; Kremer, Shelly; Laut, Megan

    2012-01-01

    Avian surveys were conducted on the islands of Tinian and Aguiguan, Marianas Islands, in 2008 by the U.S. Fish and Wildlife Service to provide current baseline densities and abundances and assess population trends using data collected from previous surveys. On Tinian, during the three surveys (1982, 1996, and 2008), 18 species were detected, and abundances and trends were assessed for 12 species. Half of the 10 native species—Yellow Bittern (Ixobrychus sinensis), White-throated Ground-Dove (Gallicolumba xanthonura), Collared Kingfisher (Todiramphus chloris), Rufous Fantail (Rhipidura rufifrons), and Micronesian Starling (Aplonis opaca)—and one alien bird—Island Collared-Dove (Streptopelia bitorquata)—have increased since 1982. Three native birds—Mariana Fruit-Dove (Ptilinopus roseicapilla), Micronesian Honeyeater (Myzomela rubratra), and Tinian Monarch (Monarcha takatsukasae)—have decreased since 1982. Trends for the remaining two native birds—White Tern (Gygis alba) and Bridled White-eye (Zosterops saypani)—and one alien bird—Eurasian Tree Sparrow (Passer montanus)—were considered relatively stable. Only five birds—White-throated Ground-Dove, Mariana Fruit-Dove, Tinian Monarch, Rufous Fantail, and Bridled White-eye—showed significant differences among regions of Tinian by year. Tinian Monarch was found in all habitat types, with the greatest monarch densities observed in limestone forest, secondary forest, and tangantangan (Leucaena leucocephala) thicket and the smallest densities found in open fields and urban/residential habitats. On Aguiguan, 19 species were detected on one or both of the surveys (1982 and 2008), and abundance estimates were produced for nine native and one alien species. Densities for seven of the nine native birds—White-throated Ground-Dove, Mariana Fruit-Dove, Collared Kingfisher, Rufous Fantail, Bridled White-eye, Golden White-eye (Cleptornis marchei), and Micronesian Starling—and the alien bird— Island

  13. An assessment of health and disease in the prehistoric inhabitants of the Mariana Islands.

    PubMed

    Pietrusewsky, M; Douglas, M T; Ikehara-Quebral, R M

    1997-11-01

    Using a variety of skeletal and dental stress indicators, an assessment of the health and disease of the indigenous inhabitants of the Mariana Islands, the Chamorro, is made. The major hypothesis to be tested is that the Chamorro were relatively healthy and that deviations from the expected, as well as inter-island variation, may reflect environmental, ecological, and cultural differences. The major skeletal series surveyed include sites on Guam (N = 247 individuals), Rota (N = 14), Tinian (N = 20), and Saipan (N = 102). The majority of the specimens are from the transitional pre-Latte (AD 1-1000) and Latte (AD 1000-1521) periods. These data derive primarily from unpublished osteological reports. The indicators of health and disease surveyed include mortality and paleodemographic data, stature, dental paleopathology, cribra orbitalia, limb bone fractures, degenerative osteoarthritis, and infectious disease (including treponemal infection). Where appropriate, tests of significance are calculated to determine the presence of any patterning in the differences observed within and between the skeletal series. Information recorded in prehistoric Hawaiians provides a standard for external comparisons. Several of the larger skeletal series surveyed have paleodemographic features that are consistent with long-term cemetery populations. Females and subadults are typically underrepresented. Most subadult deaths occur in the 2-5 year age interval. Life expectancy at birth ranges from 26.4 to 33.7 years. A healthy fertility rate is indicated for these series. The prehistoric inhabitants of the Mariana Islands were relatively tall, exceeding living Chamorros measured in the early part of the present century. The greater prevalence of developmental defects in the enamel suggests that the Chamorro were exposed to more stress than prehistoric Hawaiians. The low frequency of cribra orbitalia further indicates iron deficiency anemia was not a problem. There are generally low

  14. Geochemistry of southern Pagan Island lavas, Mariana arc: The role of subduction zone processes

    USGS Publications Warehouse

    Marske, J.P.; Pietruszka, A.J.; Trusdell, F.A.; Garcia, M.O.

    2011-01-01

    New major and trace element abundances, and Pb, Sr, and Nd isotopic ratios of Quaternary lavas from two adjacent volcanoes (South Pagan and the Central Volcanic Region, or CVR) located on Pagan Island allow us to investigate the mantle source (i.e., slab components) and melting dynamics within the Mariana intra-oceanic arc. Geologic mapping reveals a pre-caldera (780-9.4ka) and post-caldera (<9.4ka) eruptive stage for South Pagan, whereas the eruptive history of the older CVR is poorly constrained. Crystal fractionation and magma mixing were important crustal processes for lavas from both volcanoes. Geochemical and isotopic variations indicate that South Pagan and CVR lavas, and lavas from the northern volcano on the island, Mt. Pagan, originated from compositionally distinct parental magmas due to variations in slab contributions (sediment and aqueous fluid) to the mantle wedge and the extent of mantle partial melting. A mixing model based on Pb and Nd isotopic ratios suggests that the average amount of sediment in the source of CVR (~2.1%) and South Pagan (~1.8%) lavas is slightly higher than Mt. Pagan (~1.4%) lavas. These estimates span the range of sediment-poor Guguan (~1.3%) and sediment-rich Agrigan (~2.0%) lavas for the Mariana arc. Melt modeling demonstrates that the saucer-shaped normalized rare earth element (REE) patterns observed in Pagan lavas can arise from partial melting of a mixed source of depleted mantle and enriched sediment, and do not require amphibole interaction or fractionation to depress the middle REE abundances of the lavas. The modeled degree of mantle partial melting for Agrigan (2-5%), Pagan (3-7%), and Guguan (9-15%) lavas correlates with indicators of fluid addition (e.g., Ba/Th). This relationship suggests that the fluid flux to the mantle wedge is the dominant control on the extent of partial melting beneath Mariana arc volcanoes. A decrease in the amount of fluid addition (lower Ba/Th) and extent of melting (higher Sm/Yb), and

  15. Esmeralda Bank: Geochemistry of an active submarine volcano in the Mariana Island Arc

    NASA Astrophysics Data System (ADS)

    Stern, Robert J.; Bibee, L. D.

    1984-05-01

    Esmeralda Bank is the southernmost active volcano in the Izu-Volcano-Mariana Arc. This submarine volcano is one of the most active vents in the western Pacific. It has a total volume of about 27 km3, rising to within 30 m of sea level. Two dredge hauls from Esmeralda recovered fresh, nearly aphyric, vesicular basalts and basaltic andesites and minor basaltic vitrophyre. These samples reflect uniform yet unusual major and trace element chemistries. Mean abundances of TiO2 (1.3%) and FeO* (12.6%) are higher and CaO (9.2%) and Al2O3 (15.1%) are lower than rocks of similar silica content from other active Mariana Arc volcanoes. Mean incompatible element ratios K/Rb (488) and K/Ba (29) of Esmeralda rocks are indistinguishable from those of other Mariana Arc volcanoes. On a Ti-Zr plot, Esmeralda samples plot in the field of oceanic basalts while other Mariana Arc volcanic rocks plot in the field for island arcs. Incompatible element ratios K/Rb and K/Ba and isotopic compositions of Sr (87Sr/86Sr=0.70342 0.70348), Nd (ɛND=+7.6 to +8.1), and O(δ18O=+5.8 to +5.9) are incompatible with models calling for the Esmeralda source to include appreciable contributions from pelagic sediments or fresh or altered abyssal tholeiite from subduction zone melting. Instead, incompatible element and isotopic ratios of Esmeralda rocks are similar to those of intra-plate oceanic islands or “hot-spot” volcanoes in general and Kilauean tholeiites in particular. The conclusion that the source for Esmeralda lavas is an ocean-island type mantle reservoir is preferred. Esmeralda Bank rare earth element patterns are inconsistent with models calling for residual garnet in the source region, but are adequately modelled by 7 10% equilibrium partial melting of spinel lherzolite. This is supported by consideration of the results of melting experiments at 20 kbars, 1,150° C with CO2 and H2O as important volatile components. These experiments further indicate that low MgO (4.1%), MgO/FeO*(0.25) and

  16. Apoplastic Peroxidases and Lignification in Needles of Norway Spruce (Picea abies L.).

    PubMed Central

    Polle, A.; Otter, T.; Seifert, F.

    1994-01-01

    The objective of the present study was to investigate the correlation of soluble apoplastic peroxidase activity with lignification in needles of field-grown Norway spruce (Picea abies L.) trees. Apoplastic peroxidases (EC 1.11.1.7) were obtained by vacuum infiltration of needles. The lignin content of isolated cell walls was determined by the acetyl bromide method. Accumulation of lignin and seasonal variations of apoplastic peroxidase activities were studied in the first year of needle development. The major phase of lignification started after bud break and was terminated about 4 weeks later. This phase correlated with a transient increase in apoplastic guaiacol and coniferyl alcohol peroxidase activity. NADH oxidase activity, which is thought to sustain peroxidase activity by production of H2O2, peaked sharply after bud break and decreased during the lignification period. Histochemical localization of peroxidase with guaiacol indicated that high activities were present in lignifying cell walls. In mature needles, lignin was localized in walls of most needle tissues including mesophyll cells, and corresponded to 80 to 130 [mu]mol lignin monomers/g needle dry weight. Isoelectric focusing of apoplastic washing fluids and activity staining with guaiacol showed the presence of strongly alkaline peroxidases (isoelectric point [greater than or equal to] 9) in all developmental stages investigated. New isozymes with isoelectric points of 7.1 and 8.1 appeared during the major phase of lignification. These isozymes disappeared after lignification was terminated. A strong increase in peroxidase activity in autumn was associated with the appearance of acidic peroxidases (isoelectric point [less than or equal to] 3). These results suggest that soluble alkaline apoplastic peroxidases participate in lignin formation. Soluble acidic apoplastic peroxidases were apparently unrelated to developmentally regulated lignification in spruce needles. PMID:12232302

  17. Bacteriocyte-associated gammaproteobacterial symbionts of the Adelges nordmannianae/piceae complex (Hemiptera: Adelgidae)

    PubMed Central

    Toenshoff, Elena R; Penz, Thomas; Narzt, Thomas; Collingro, Astrid; Schmitz-Esser, Stephan; Pfeiffer, Stefan; Klepal, Waltraud; Wagner, Michael; Weinmaier, Thomas; Rattei, Thomas; Horn, Matthias

    2012-01-01

    Adelgids (Insecta: Hemiptera: Adelgidae) are known as severe pests of various conifers in North America, Canada, Europe and Asia. Here, we present the first molecular identification of bacteriocyte-associated symbionts in these plant sap-sucking insects. Three geographically distant populations of members of the Adelges nordmannianae/piceae complex, identified based on coI and ef1alpha gene sequences, were investigated. Electron and light microscopy revealed two morphologically different endosymbionts, coccoid or polymorphic, which are located in distinct bacteriocytes. Phylogenetic analyses of their 16S and 23S rRNA gene sequences assigned both symbionts to novel lineages within the Gammaproteobacteria sharing <92% 16S rRNA sequence similarity with each other and showing no close relationship with known symbionts of insects. Their identity and intracellular location were confirmed by fluorescence in situ hybridization, and the names ‘Candidatus Steffania adelgidicola' and ‘Candidatus Ecksteinia adelgidicola' are proposed for tentative classification. Both symbionts were present in all individuals of all investigated populations and in different adelgid life stages including eggs, suggesting vertical transmission from mother to offspring. An 85 kb genome fragment of ‘Candidatus S. adelgidicola' was reconstructed based on a metagenomic library created from purified symbionts. Genomic features including the frequency of pseudogenes, the average length of intergenic regions and the presence of several genes which are absent in other long-term obligate symbionts, suggested that ‘Candidatus S. adelgidicola' is an evolutionarily young bacteriocyte-associated symbiont, which has been acquired after diversification of adelgids from their aphid sister group. PMID:21833037

  18. Genetic signatures of natural selection in response to air pollution in red spruce (Picea rubens, Pinaceae).

    PubMed

    Bashalkhanov, Stanislav; Eckert, Andrew J; Rajora, Om P

    2013-12-01

    One of the most important drivers of local adaptation for forest trees is climate. Coupled to these patterns, however, are human-induced disturbances through habitat modification and pollution. The confounded effects of climate and disturbance have rarely been investigated with regard to selective pressure on forest trees. Here, we have developed and used a population genetic approach to search for signals of selection within a set of 36 candidate genes chosen for their putative effects on adaptation to climate and human-induced air pollution within five populations of red spruce (Picea rubens Sarg.), distributed across its natural range and air pollution gradient in eastern North America. Specifically, we used FST outlier and environmental correlation analyses to highlight a set of seven single nucleotide polymorphisms (SNPs) that were overly correlated with climate and levels of sulphate pollution after correcting for the confounding effects of population history. Use of three age cohorts within each population allowed the effects of climate and pollution to be separated temporally, as climate-related SNPs (n = 7) showed the strongest signals in the oldest cohort, while pollution-related SNPs (n = 3) showed the strongest signals in the youngest cohorts. These results highlight the usefulness of population genetic scans for the identification of putatively nonneutral evolution within genomes of nonmodel forest tree species, but also highlight the need for the development and application of robust methodologies to deal with the inherent multivariate nature of the genetic and ecological data used in these types of analyses.

  19. SABATH methyltransferases from white spruce (Picea glauca): gene cloning, functional characterization and structural analysis.

    PubMed

    Zhao, Nan; Boyle, Brian; Duval, Isabelle; Ferrer, Jean-Luc; Lin, Hong; Seguin, Armand; MacKay, John; Chen, Feng

    2009-07-01

    Known members of the plant SABATH family of methyltransferases have important biological functions by methylating hormones, signalling molecules and other metabolites. While all previously characterized SABATH genes were isolated from angiosperms, in this article, we report on the isolation and functional characterization of SABATH genes from white spruce (Picea glauca [Moench] Voss), a gymnosperm. Through EST database search, three genes that encode proteins significantly homologous to known SABATH proteins were identified from white spruce. They were named PgSABATH1, PgSABATH2 and PgSABATH3, respectively. Full length cDNAs of these three genes were cloned and expressed in Escherichia coli. The E. coli-expressed recombinant proteins were tested for methyltransferase activity with a large number of compounds. While no activity was detected for PgSABATH2 and PgSABATH3, PgSABATH1 displayed the highest level of catalytic activity with indole-3-acetic acid (IAA). PgSABATH1 was, therefore, renamed PgIAMT1. Under steady-state conditions, PgIAMT1 exhibited apparent Km values of 18.2 microM for IAA. Homology-based structural modelling of PgIAMT1 revealed that the active site of PgIAMT1 is highly similar to other characterized IAMTs from angiosperms. PgIAMT1 showed expression in multiple tissues, with the highest level of expression detected in embryonic tissues. During somatic embryo maturation, a significant reduction in PgIAMT1 transcript levels was observed when developing cotyledons become apparent which is indicative of mature embryos. The biological roles of white spruce SABATH genes, especially those of PgIAMT1, and the evolution of the SABATH family are discussed.

  20. Does carbon availability control temporal dynamics of radial growth in Norway spruce (Picea abies)?

    NASA Astrophysics Data System (ADS)

    Oberhuber, Walter; Gruber, Andreas; Swidrak, Irene

    2015-04-01

    Intra-annual dynamics of cambial activity and wood formation of coniferous species exposed to soil dryness revealed early culmination of maximum growth in late spring prior to occurrence of more favourable environmental conditions, i.e., repeated high rainfall events during summer (Oberhuber et al. 2014). Because it is well known that plants can adjust carbon allocation patterns to optimize resource uptake under prevailing environmental constraints, we hypothesize that early decrease in radial stem growth is an adaptation to cope with drought stress, which might require an early switch of carbon allocation to belowground organs. Physical blockage of carbon transport in the phloem through girdling causes accumulation and depletion of carbohydrates above and below the girdle, respectively, making this method quite appropriate to investigate carbon relationships in trees. Hence, in a common garden experiment we will manipulate the carbon status of Norway spruce (Picea abies) saplings by phloem blockage at different phenological stages during the growing season. We will present the methodological approach and first results of the study aiming to test the hypothesis that carbon status of the tree affects temporal dynamics of cambial activity and wood formation in conifers under drought. Acknowledgment The research is funded by the Austrian Science Fund (FWF): P25643-B16 "Carbon allocation and growth of Scots pine". Reference Oberhuber W, A Gruber, W Kofler, I Swidrak (2014) Radial stem growth in response to microclimate and soil moisture in a drought-prone mixed coniferous forest at an inner Alpine site. Eur J For Res 133:467-479.

  1. In Planta Localization of Stilbenes within Picea abies Phloem1[OPEN

    PubMed Central

    Kuroda, Katsushi; Pranovich, Andrey; Roig-Juan, Sílvia; Aoki, Dan

    2016-01-01

    Phenolic stilbene glucosides (astringin, isorhapontin, and piceid) and their aglycons commonly accumulate in the phloem of Norway spruce (Picea abies). However, current knowledge about the localization and accumulation of stilbenes within plant tissues and cells remains limited. Here, we used an innovative combination of novel microanalytical techniques to evaluate stilbenes in a frozen-hydrated condition (i.e. in planta) and a freeze-dried condition across phloem tissues. Semiquantitative time-of-flight secondary ion-mass spectrometry imaging in planta revealed that stilbenes were localized in axial parenchyma cells. Quantitative gas chromatography analysis showed the highest stilbene content in the middle of collapsed phloem with decreases toward the outer phloem. The same trend was detected for soluble sugar and water contents. The specimen water content may affect stilbene composition; the glucoside-to-aglycon ratio decreased slightly with decreases in water content. Phloem chemistry was correlated with three-dimensional structures of phloem as analyzed by microtomography. The outer phloem was characterized by a high volume of empty parenchyma, reduced ray volume, and a large number of axial parenchyma with porous vacuolar contents. Increasing porosity from the inner to the outer phloem was related to decreasing compactness of stilbenes and possible secondary oxidation or polymerization. Our results indicate that aging-dependent changes in phloem may reduce cell functioning, which affects the capacity of the phloem to store water and sugar, and may reduce the defense potential of stilbenes in the axial parenchyma. Our results highlight the power of using a combination of techniques to evaluate tissue- and cell-level mechanisms involved in plant secondary metabolite formation and metabolism. PMID:27531441

  2. Critically reduced frost resistance of Picea abies during sprouting could be linked to cytological changes.

    PubMed

    Neuner, G; Beikircher, B

    2010-07-01

    Frost resistance of sprouting Picea abies shoots is insufficient for survival of naturally occurring late frosts. The cellular changes during sprouting appeared to be responsible for frost damage as frost events that damaged sprouting shoots did not damage older needles and stems. Whilst resting buds showed initial frost damage at -15.0 degrees C, 20 days later, current year's growth was damaged at -5.6 degrees C. The decrease in frost resistance in sprouting shoots of P. abies was accompanied by a significant reduction of the cellular solute concentration, indicated by much less negative Psi(oSAT) values (increase from -2.8 to -1.2 MPa). psi(oSAT) decreased again after the final cell volume was reached and cell wall thickening began. After bud break, ice nucleation temperature increased from -4.7 degrees C to -1.5 degrees C. This increase was probably caused by the loss of bud scales, the onset of expansion growth of the central cylinder and the development of vascular tissue permitting the spread of ice from the stem into the growing needles. The onset of mesophyll cell wall thickening coincided with the lowest frost resistances. Cell wall thickening caused an increase in the modulus of elasticity, epsilon, indicating a decrease in tissue elasticity and after that frost resistance increased again. Metabolic and cytological changes that evidently leave little leeway for frost hardening are responsible for the low frost resistance in current year's growth of P. abies. This low frost resistance will be significant in the future as the risk of frost damage due to earlier bud break is anticipated to even further increase.

  3. Single-Step BLUP with Varying Genotyping Effort in Open-Pollinated Picea glauca.

    PubMed

    Ratcliffe, Blaise; El-Dien, Omnia Gamal; Cappa, Eduardo P; Porth, Ilga; Klápště, Jaroslav; Chen, Charles; El-Kassaby, Yousry A

    2017-03-10

    Maximization of genetic gain in forest tree breeding programs is contingent on the accuracy of the predicted breeding values and precision of the estimated genetic parameters. We investigated the effect of the combined use of contemporary pedigree information and genomic relatedness estimates on the accuracy of predicted breeding values and precision of estimated genetic parameters, as well as rankings of selection candidates, using single-step genomic evaluation (HBLUP). In this study, two traits with diverse heritabilities [tree height (HT) and wood density (WD)] were assessed at various levels of family genotyping efforts (0, 25, 50, 75, and 100%) from a population of white spruce (Picea glauca) consisting of 1694 trees from 214 open-pollinated families, representing 43 provenances in Québec, Canada. The results revealed that HBLUP bivariate analysis is effective in reducing the known bias in heritability estimates of open-pollinated populations, as it exposes hidden relatedness, potential pedigree errors, and inbreeding. The addition of genomic information in the analysis considerably improved the accuracy in breeding value estimates by accounting for both Mendelian sampling and historical coancestry that were not captured by the contemporary pedigree alone. Increasing family genotyping efforts were associated with continuous improvement in model fit, precision of genetic parameters, and breeding value accuracy. Yet, improvements were observed even at minimal genotyping effort, indicating that even modest genotyping effort is effective in improving genetic evaluation. The combined utilization of both pedigree and genomic information may be a cost-effective approach to increase the accuracy of breeding values in forest tree breeding programs where shallow pedigrees and large testing populations are the norm.

  4. Single-Step BLUP with Varying Genotyping Effort in Open-Pollinated Picea glauca

    PubMed Central

    Ratcliffe, Blaise; El-Dien, Omnia Gamal; Cappa, Eduardo P.; Porth, Ilga; Klápště, Jaroslav; Chen, Charles; El-Kassaby, Yousry A.

    2017-01-01

    Maximization of genetic gain in forest tree breeding programs is contingent on the accuracy of the predicted breeding values and precision of the estimated genetic parameters. We investigated the effect of the combined use of contemporary pedigree information and genomic relatedness estimates on the accuracy of predicted breeding values and precision of estimated genetic parameters, as well as rankings of selection candidates, using single-step genomic evaluation (HBLUP). In this study, two traits with diverse heritabilities [tree height (HT) and wood density (WD)] were assessed at various levels of family genotyping efforts (0, 25, 50, 75, and 100%) from a population of white spruce (Picea glauca) consisting of 1694 trees from 214 open-pollinated families, representing 43 provenances in Québec, Canada. The results revealed that HBLUP bivariate analysis is effective in reducing the known bias in heritability estimates of open-pollinated populations, as it exposes hidden relatedness, potential pedigree errors, and inbreeding. The addition of genomic information in the analysis considerably improved the accuracy in breeding value estimates by accounting for both Mendelian sampling and historical coancestry that were not captured by the contemporary pedigree alone. Increasing family genotyping efforts were associated with continuous improvement in model fit, precision of genetic parameters, and breeding value accuracy. Yet, improvements were observed even at minimal genotyping effort, indicating that even modest genotyping effort is effective in improving genetic evaluation. The combined utilization of both pedigree and genomic information may be a cost-effective approach to increase the accuracy of breeding values in forest tree breeding programs where shallow pedigrees and large testing populations are the norm. PMID:28122953

  5. Evaluation of growth disturbances of Picea abies (L.) Karst. to disturbances caused by landslide movements

    NASA Astrophysics Data System (ADS)

    Šilhán, Karel

    2017-01-01

    Dendrogeomorphic methods are frequently used in landslide analyses. Although methods of landslide dating based on tree rings are well developed, they still indicated many questions. The aim of this study was to evaluate the frequently used theoretical scheme based on the event-response relationship. Seventy-four individuals of Norway spruce (Picea abies (L.) Karst.) exhibiting visible external disturbance, were sampled on the Girová landslide (the largest historical flow-like landslide in the Czech Republic). This landslide reactivated in May 2010, and post-landslide tree growth responses were studied in detail. These growth responses were compared with the intensity and occurrence of visible external tree disturbance: tilted stems, damaged root systems, and decapitation. Twenty-nine trees (39.2%) died within one to four years following the 2010 landslide movement. The trees that died following the landslide movement were significantly younger and displayed significantly greater stem tilting than the live trees. Abrupt growth suppression was a more-frequent response among the dead trees, whereas growth release dominated among the live trees. Only two trees (2.7%) created no reaction wood in response to the landslide movement. Forty-four percent of the trees started to produce reaction wood structure after a delay, which generally spanned one year. Some eccentric growth was evident in the tree rings of the landslide year and was significant in the first years following the landslide movement. Missing rings were observed only on the upper sides of the stems, and no false tree rings were observed. The results confirm the general validity of event-response relationship, nevertheless this study points out the limitations and uncertainties of this generally accepted working scheme.

  6. Genome Sequence of the Deep-Sea Denitrifier Pseudomonas sp. Strain MT-1, Isolated from the Mariana Trench.

    PubMed

    Fujinami, Shun; Oikawa, Yuji; Araki, Takuma; Shinmura, Yui; Midorikawa, Ryota; Ishizaka, Hikari; Kato, Chiaki; Horikoshi, Koki; Ito, Masahiro; Tamegai, Hideyuki

    2014-12-18

    Pseudomonas sp. strain MT-1 was the first deep-sea denitrifier isolated and characterized from mud recovered from a depth of 11,000 m in the Mariana Trench. We report here the genome sequence of this bacterium, which contributes to our understanding of denitrification and bioenergetics in the deep sea.

  7. 26 CFR 1.876-1 - Alien residents of Puerto Rico, Guam, American Samoa, or the Northern Mariana Islands.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 26 Internal Revenue 9 2014-04-01 2014-04-01 false Alien residents of Puerto Rico, Guam, American Samoa, or the Northern Mariana Islands. 1.876-1 Section 1.876-1 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED) INCOME TAX (CONTINUED) INCOME TAXES (CONTINUED) Nonresident Aliens and Foreign Corporations §...

  8. 26 CFR 1.876-1 - Alien residents of Puerto Rico, Guam, American Samoa, or the Northern Mariana Islands.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 26 Internal Revenue 9 2012-04-01 2012-04-01 false Alien residents of Puerto Rico, Guam, American Samoa, or the Northern Mariana Islands. 1.876-1 Section 1.876-1 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED) INCOME TAX (CONTINUED) INCOME TAXES (CONTINUED) Nonresident Aliens and Foreign Corporations §...

  9. 26 CFR 1.876-1 - Alien residents of Puerto Rico, Guam, American Samoa, or the Northern Mariana Islands.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 26 Internal Revenue 9 2013-04-01 2013-04-01 false Alien residents of Puerto Rico, Guam, American Samoa, or the Northern Mariana Islands. 1.876-1 Section 1.876-1 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED) INCOME TAX (CONTINUED) INCOME TAXES (CONTINUED) Nonresident Aliens and Foreign Corporations §...

  10. 26 CFR 1.876-1 - Alien residents of Puerto Rico, Guam, American Samoa, or the Northern Mariana Islands.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 26 Internal Revenue 9 2010-04-01 2010-04-01 false Alien residents of Puerto Rico, Guam, American Samoa, or the Northern Mariana Islands. 1.876-1 Section 1.876-1 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED) INCOME TAX (CONTINUED) INCOME TAXES Nonresident Aliens and Foreign Corporations § 1.876-1 Alien...

  11. 33 CFR 334.1420 - Pacific Ocean off Orote Point, Apra Harbor, Island of Guam, Marianas Islands; small arms firing...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 33 Navigation and Navigable Waters 3 2013-07-01 2013-07-01 false Pacific Ocean off Orote Point, Apra Harbor, Island of Guam, Marianas Islands; small arms firing range. 334.1420 Section 334.1420... AND RESTRICTED AREA REGULATIONS § 334.1420 Pacific Ocean off Orote Point, Apra Harbor, Island of...

  12. 33 CFR 334.1420 - Pacific Ocean off Orote Point, Apra Harbor, Island of Guam, Marianas Islands; small arms firing...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 33 Navigation and Navigable Waters 3 2012-07-01 2012-07-01 false Pacific Ocean off Orote Point, Apra Harbor, Island of Guam, Marianas Islands; small arms firing range. 334.1420 Section 334.1420... AND RESTRICTED AREA REGULATIONS § 334.1420 Pacific Ocean off Orote Point, Apra Harbor, Island of...

  13. A Study of Risk Factors among High School Students in the Commonwealth of the Northern Mariana Islands.

    ERIC Educational Resources Information Center

    Kawakami, Alice J.; And Others

    The status of students at risk of failure in public high schools in the Commonwealth of the Northern Mariana Islands (CNMI) was studied during the 1993-94 school year as part of a larger study of at-risk students in some of the American-affiliated Pacific political entities. In the CNMI, data were collected from 57 student records, and interviews…

  14. 9 CFR 72.3 - Areas quarantined in the Virgin Islands of the United States, the Northern Mariana Islands, the...

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... Islands of the United States, the Northern Mariana Islands, the Commonwealth of Puerto Rico, and the Island of Guam. 72.3 Section 72.3 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE... (SPLENETIC) FEVER IN CATTLE § 72.3 Areas quarantined in the Virgin Islands of the United States, the...

  15. 33 CFR 334.1420 - Pacific Ocean off Orote Point, Apra Harbor, Island of Guam, Marianas Islands; small arms firing...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 3 2010-07-01 2010-07-01 false Pacific Ocean off Orote Point, Apra Harbor, Island of Guam, Marianas Islands; small arms firing range. 334.1420 Section 334.1420... AND RESTRICTED AREA REGULATIONS § 334.1420 Pacific Ocean off Orote Point, Apra Harbor, Island of...

  16. 33 CFR 334.1420 - Pacific Ocean off Orote Point, Apra Harbor, Island of Guam, Marianas Islands; small arms firing...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 3 2011-07-01 2011-07-01 false Pacific Ocean off Orote Point, Apra Harbor, Island of Guam, Marianas Islands; small arms firing range. 334.1420 Section 334.1420... AND RESTRICTED AREA REGULATIONS § 334.1420 Pacific Ocean off Orote Point, Apra Harbor, Island of...

  17. 26 CFR 1.935-1 - Coordination of individual income taxes with Guam and the Northern Mariana Islands.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 26 Internal Revenue 10 2010-04-01 2010-04-01 false Coordination of individual income taxes with... United States § 1.935-1 Coordination of individual income taxes with Guam and the Northern Mariana... this rule is to facilitate the coordination of the tax systems of the United States and the...

  18. Peroxidase Activity in Relation to Suberization and Respiration in White Spruce (Picea glauca [Moench] Voss) Seedling Roots 1

    PubMed Central

    Johnson-Flanagan, Anne M.; Owens, John N.

    1985-01-01

    Peroxidase (EC 1.11.1.7) activity is associated with suberization during endodermal development and metacutization in roots of white spruce (Picea glauca [Moench] Voss) seedlings. Histochemical analysis indicates a relationship between suberization and peroxidase activity, but peroxidase is ubiquitous. Increased peroxidase activity results from the induction of four anodic peroxidase isozymes in addition to quantitative increases in two anodic peroxidase isozymes. Four of these polymerized eugenol. Cold temperatures induce formation of two anodic isozymes and result in suberization. The increased peroxidase activity associated with suberization is correlated to residual respiration. In an attempt to elucidate this relationship, the effect of respiratory inhibitors on respiration and peroxidase activity are compared. PMID:16664352

  19. Identification of Gibberellins in Norway Spruce (Picea abies [L.] Karst.) by Combined Gas Chromatography-Mass Spectrometry

    PubMed Central

    Odén, Per Christer; Schwenen, Ludger; Graebe, Jan E.

    1987-01-01

    Gibberellins A1 (GA1), A3 and A9 were identified from extracts of shoots of 6-month old Norway spruce (Picea abies) seedlings by the use of sequential reverse and normal phase high performance liquid chromatography (HPLC), bioassay, radioimmunoassay (RIA) and combined gas chromatography-mass spectrometry (GC-MS). The bioassay and RIA were used after fractionation by HPLC to detect the GA-containing fractions, which were then examined by GC-MS. The GAs identified are considered to be endogenous. PMID:16665471

  20. Seismic structure and seismicity at the southern Mariana Trough with hydrothermal activities

    NASA Astrophysics Data System (ADS)

    Sato, T.; Mizuno, M.; Takada, H.; Yamada, T.; Isse, T.; Shinohara, M.

    2012-12-01

    The Southern Mariana Trough back-arc spreading system shows asymmetry spreading, and has high relief at spreading axes, which infers abundant melt supply. Furthermore, five hydrothermal vents that extrude different water contents, exist within 5 km near the spreading axis. To investigate upper mantle structure, crustal structure and hypocenter distribution provide important constraint on following four main points to understand the back-arc spreading system; 1) imaging melt delivery to the spreading axis and off axis seamount including volcanic arc, 2) production and character of the crust, 3) relationship between melt supply and crustal formation, and 4) pathway and heat source for hydrothermal circulation with related to its formation. We conducted a seismic reflection/refraction survey and seismicity observation at the hydrothermal area in the Southern Mariana Trough from August to November in 2010. We used 9 ocean bottom seismometers, an air gun (GI gun) and a single channel streamer cable. We took 7 parallel lines and 7 perpendicular lines to the spreading center. Line length was 15 km each, and line interval was 2.5 km. From the survey and observation, we obtained very low seismicity at the hydrothermal area in the 3 month's observation. The reflection survey shows that some reflectors exist under the hydrothermal area. In this presentation, we will also show seismic velocity structures from the refraction survey.

  1. Numerical Modeling of Sound from the Eruption of Anatahan Volcano, Mariana Islands

    NASA Astrophysics Data System (ADS)

    Park, M.; Dziak, R. P.; Byun, S.; Fox, C. G.; Matsumoto, H.

    2003-12-01

    NOAA VENTS Program deployed an array of five autonomous underwater hydrophones within the SOFAR channel along the Mariana chain in February 2003 to monitor seafloor volcanic eruptions and submarine earthquakes (sponsored by NOAA's Ocean Exploration Program). These five hydrophones will be recovered in September 2003 using KORDI R/V Onnuri. The first historical eruption of Anatahan volcano in the Mariana Islands began on 10 May 2003. It is expected that the hydrophone data will include the hydroacoustic records of the eruption of Anatahan Volcano. The signals recorded from the eruption will be numerically modeled using a T-wave excitation mechanism developed from the mode scattering theory of Park et al. (2001). They found that scattering from the rough seabottom converts the acoustic energy of seafloor earthquakes from the directly excited ocean crustal/water column modes to the propagating acoustic modes of T-waves, and developed an algorithm to numerically model oceanic earthquake's T-waves. We modified this numerical model of Park et al. (2001) to predict the T-waves generated from volcanic sources by adopting a buried magmatic pipe model (Chouet, 1985). We derived a moment-tensor representation of a volcano-seismic source that is governed by the geometry of the source and the physical properties of magma. Numerical modeling of the sound from the eruption requires us to determine governing factors such as the pipe radius and magma viscosity that will enable us to grasp the inward nature of Anatahan volcano.

  2. Community Structure Comparisons of Hydrothermal Vent Microbial Mats Along the Mariana Arc and Back-arc

    NASA Astrophysics Data System (ADS)

    Hager, K. W.; Fullerton, H.; Moyer, C. L.

    2015-12-01

    Hydrothermal vents along the Mariana Arc and back-arc represent a hotspot of microbial diversity that has not yet been fully recognized. The Mariana Arc and back-arc contain hydrothermal vents with varied vent effluent chemistry and temperature, which translates to diverse community composition. We have focused on iron-rich sites where the dominant primary producers are iron oxidizing bacteria. Because microbes from these environments have proven elusive in culturing efforts, we performed culture independent analysis among different microbial communities found at these hydrothermal vents. Terminal-restriction fragment length polymorphism (T-RFLP) and Illumina sequencing of small subunit ribosomal gene amplicons were used to characterize community members and identify samples for shotgun metagenomics. Used in combination, these methods will better elucidate the composition and characteristics of the bacterial communities at these hydrothermal vent systems. The overarching goal of this study is to evaluate and compare taxonomic and metabolic diversity among different communities of microbial mats. We compared communities collected on a fine scale to analyze the bacterial community based on gross mat morphology, geography, and nearby vent effluent chemistry. Taxa richness and evenness are compared with rarefaction curves to visualize diversity. As well as providing a survey of diversity this study also presents a juxtaposition of three methods in which ribosomal small subunit diversity is compared with T-RFLP, next generation amplicon sequencing, and metagenomic shotgun sequencing.

  3. Temporal Evolution of the Mariana Arc: Mantle Wedge and Subducted Slab Controls Revealed with a Tephra Perspective

    NASA Astrophysics Data System (ADS)

    Straub, S. M.; Woodhead, J. D.; Arculus, R. J.

    2014-12-01

    Tephra recovered by deep-sea drilling from forearc to backarc across the Mariana volcanic arc system record the last 34 million years of the Arc's evolution. Major and trace element abundances and Sr-Nd-Pb-Hf isotope ratios have been determined for tephra with high temporal precision and an average resolution of ˜1 million years. Temporal variations of source-sensitive radiogenic isotopes and large-ion lithophile elements (LILE) are decoupled from the steady trends of silica and other archetypical crust-forming major elements. Modeling confirms the temporal isotopic and elemental abundance trends are controlled by subducted slab and mantle sources. Pb and Sr fluxes can be linked to fluids from altered oceanic crust (AOC), Nd is influenced by contributions from the mantle wedge and partial slab melts, while Hf mostly derives from the subarc mantle. Most plausibly, the K2O increase and fluctuations thereafter can be linked to a collision of the Mariana Arc with the leading trace of the Cretaceous-aged Western Pacific Seamount Province. The Province is inferred to have arrived at the Mariana Trench at ˜15-16 Ma coincident with the termination of spreading in the Parece Vela Backarc Basin. A short period of slab melting followed, possibly induced by slab rollback that peaked at ˜8-9 Ma and ended with the incipient rifting in the Mariana Trough at ˜7 Ma. Individual periods of Arc formation (52-24, 22-11, and 10-0 Ma) are characterized by a distinctive melee of source materials which is not repeated through time. Mariana Arc crustal growth has occurred through the addition of predominantly mafic and silicic melts formed during relatively short time intervals traceable via the chemically-distinctive subducted slab inputs.

  4. The Origins and Genetic Distinctiveness of the Chamorros of the Marianas Islands: An mtDNA Perspective

    PubMed Central

    VILAR, MIGUEL G.; CHAN, CHIM W; SANTOS, DANA R; LYNCH, DANIEL; SPATHIS, RITA; GARRUTO, RALPH M; LUM, J KOJI

    2013-01-01

    Background Archaeological and linguistic evidence suggests the Marianas Islands were settled around 3,600 years before present (ybp) from Island Southeast Asia (ISEA). Around 1,000 ybp latte stone pillars and the first evidence of rice cultivation appear in the Marianas. Both traditions are absent in the rest of prehistoric Oceania. Objective To examine the genetic origins and postsettlement gene flow of Chamorros of the Marianas Islands. Methods To infer the origins of the Chamorros we analyzed ~360 base pairs of the hypervariable-region 1 (HVS1) of mitochondrial DNA from 105 Chamorros from Guam, Rota, and Saipan, and the complete mitochondrial genome of 32 Guamanian Chamorros, and compared them to lineages from ISEA and neighboring Pacific archipelagoes from the database. Results Results reveal that 92% of Chamorros belong to haplogroup E, also found in ISEA but rare in Oceania. The two most numerous E lineages were identical to lineages currently found in Indonesia, while the remaining E lineages differed by only one or two mutations and all were unique to the Marianas. Seven percent of the lineages belonged to a single Chamorro-specific lineage within haplogroup B4, common to ISEA as well as Micronesia and Polynesia. Conclusions These patterns suggest a small founding population had reached and settled the Marianas from ISEA by 4,000 ybp, and developed unique mutations in isolation. A second migration from ISEA may have arrived around 1,000 ybp, introducing the latte pillars, rice agriculture and the homogeneous minority B4 lineage. PMID:23180676

  5. Mantle Inputs to the Subduction Factory: Detailed Studies of the Southern Mariana Seamount Province

    NASA Astrophysics Data System (ADS)

    Bloomer, S. H.; Stern, R. J.

    2001-12-01

    The southern Mariana Arc system has been studied intensively, with a focus on volcanoes along the magmatic arc and associated cross-chains. Sonar back-scatter imagery of about 50,000 square kilometers imaged 28 submarine volcanoes of the Mariana arc system that had not been previously studied. We also imaged the spreading axis of the Mariana Trough from 13o45' to 17 o 30', about half the length of this slow-spreading ridge. Our survey of 450 km along the arc magmatic axis, from 13 o 30'N to 17 o 20'N, indicates that these volcanoes vary widely in volume, from a third to 1000 km3. The average volcano from along the Mariana magmatic front is spaced 20km from its neighbor, is built on a platform that lies at 1800m below sealevel, rises 1000m above this platform, and occupies a volume of about 200 cubic kilometers. Lavas collected from 24 edifices along the magmatic front include abundant basalt and dacite; we also collected several cumulate gabbroic xenoliths. Phenocryst phases are dominated by plagioclase, olivine, pyroxene, and hornblende. Five of the volcanoes in the study area - the islands of Guguan, Sarigan, and Anatahan, and the submarine edifices of S. Ruby and Esmeralda -are active. Other volcanoes are extinct, and our study shows that, to a first approximation, the smaller the edifice, the less likely it is to be active. Some extinct edifices have flat summits at depths that range from 40 to 300 m which are capped with coral, or, farther north in the study area, shelly material or carbonate sand. A major cross-chain of small volcanoes along 14 o 40'N was studied and sampled for the first time. These volcanoes yielded a range of lavas, from basalt to dacite. Volcanoes from another cross-chain, extending along 17 o 20'N latitude west of Guguan, yielded only basalt. Shorter cross-chains were also studied in the Diamantes and near Sarigan. We recovered abundant pumice from 6 volcanoes in the northernmost 100km of the arc and one volcano in the south. Each

  6. Are Early Somatic Embryos of the Norway Spruce (Picea abies (L.) Karst.) Organised?

    PubMed Central

    Petrek, Jiri; Zitka, Ondrej; Adam, Vojtech; Bartusek, Karel; Anjum, Naser A.; Pereira, Eduarda; Havel, Ladislav; Kizek, Rene

    2015-01-01

    Background Somatic embryogenesis in conifer species has great potential for the forestry industry. Hence, a number of methods have been developed for their efficient and rapid propagation through somatic embryogenesis. Although information is available regarding the previous process-mediated generation of embryogenic cells to form somatic embryos, there is a dearth of information in the literature on the detailed structure of these clusters. Methodology/Principal Findings The main aim of this study was to provide a more detailed structure of the embryogenic tissue clusters obtained through the in vitro propagation of the Norway spruce (Picea abies (L.) Karst.). We primarily focused on the growth of early somatic embryos (ESEs). The data on ESE growth suggested that there may be clear distinctions between their inner and outer regions. Therefore, we selected ESEs collected on the 56th day after sub-cultivation to dissect the homogeneity of the ESE clusters. Two colourimetric assays (acetocarmine and fluorescein diacetate/propidium iodide staining) and one metabolic assay based on the use of 2,3,5-triphenyltetrazolium chloride uncovered large differences in the metabolic activity inside the cluster. Next, we performed nuclear magnetic resonance measurements. The ESE cluster seemed to be compactly aggregated during the first four weeks of cultivation; thereafter, the difference between the 1H nuclei concentration in the inner and outer clusters was more evident. There were clear differences in the visual appearance of embryos from the outer and inner regions. Finally, a cluster was divided into six parts (three each from the inner and the outer regions of the embryo) to determine their growth and viability. The innermost embryos (centripetally towards the cluster centre) could grow after sub-cultivation but exhibited the slowest rate and required the longest time to reach the common growth rate. To confirm our hypothesis on the organisation of the ESE cluster, we

  7. Life and death of Picea abies after bark-beetle outbreak: ecological processes driving seedling recruitment.

    PubMed

    Macek, Martin; Wild, Jan; Kopecký, Martin; Červenka, Jaroslav; Svoboda, Miroslav; Zenáhlíková, Jitka; Brůna, Josef; Mosandl, Reinhard; Fischer, Anton

    2017-01-01

    The severity and spatial extent of bark-beetle outbreaks substantially increased in recent decades worldwide. The ongoing controversy about natural forest recovery after these outbreaks highlights the need for individual-based long-term studies, which disentangle processes driving forest regeneration. However, such studies have been lacking. To fill this gap, we followed the fates of 2,552 individual seedlings for 12 years after a large-scale bark-beetle outbreak that caused complete canopy dieback in mountain Norway spruce (Picea abies) forests in southeast Germany. We explore the contribution of advance, disturbance-related, and post-disturbance regeneration to forest recovery. Most seedlings originated directly within the three-year dieback of canopy trees induced by bark-beetle outbreak. After complete canopy dieback, the establishment of new seedlings was minimal. Surprisingly, advance regeneration formed only a minor part of all regeneration. However, because it had the highest survival rate, its importance increased over time. The most important factor influencing the survival of seedlings after disturbance was their height. Survival was further modified by microsite: seedlings established on dead wood survived best, whereas almost all seedlings surrounded by graminoids died. For 5 cm tall seedlings, annual mortality ranged from 20 to 50% according to the rooting microsite. However, for seedlings taller than 50 cm, annual mortality was below 5% at all microsites. While microsite modified seedling mortality, it did not affect seedling height growth. A model of regeneration dynamics based on short-term observations accurately predicts regeneration height growth, but substantially underestimates mortality rate, thus predicting more surviving seedlings than were observed. We found that P. abies forests were able to regenerate naturally even after severe bark-beetle outbreaks owing to advance and particularly disturbance-related regeneration. This, together

  8. Association of ectomycorrhizal fungi with Picea crassifolia (Pinaceae, Piceoidae) from high-altitude stands in Mount Helan Nature Reserve, China.

    PubMed

    Fan, Y J; Grebenc, T; Wei, J; Zhao, Y L; Yan, W; Wang, L B

    2016-09-02

    We investigated the diversity of ectomycorrhiza associated with the endemic Picea crassifolia in Mount Helan National Nature Reserve in Inner Mongolia, China. Toward this objective, we conducted morphological and molecular identification of ectomycorrhizae in soil cubes taken from pure P. crassifolia stands. Eleven types of ectomycorrhizal (ECM) organisms were separated, briefly described, and identified. Nine morphotypes belonged to the phylum Basidiomycotina [Amphinema byssoides, Cortinarius sp (cf. limonius), Cortinarius vernus, Inocybe cf. nitidiscula, Inocybe sp 1, Sebacina incrustans, Sebacina sp, Suillus luteus, and Piceirhiza tuberculata x Picea crassifolia (comb. Nov.)], and two morphotypes to the phylum Ascomycotina (Cenococcum geophilum and Helvella sp). The diversity of ECM organisms in P. crassifolia was lower than that reported by other studies on spruce or pine forests, or on sporocarp diversity in the high-mountain forests of China. Most of the fungi in the rhizosphere did not correspond to species previously recorded as sporocarps above ground. Here, several new ectomycorrhiza morphotypes are proposed and described. We also confirmed the ectomycorrhizal status of the genus Sebacina (order Sebacinales).

  9. Impact of experimentally elevated ozone on seed germination and growth of Russian pine (Pinus sylvestris) and spruce (Picea spp.) provenances.

    PubMed

    Prozherina, Nadezda; Nakvasina, Elena; Oksanen, Elina

    2009-12-01

    The impact of elevated ozone concentrations on early ontogenetic stages of pine (Pinus sylvestris) and spruce (Picea abies, Picea obovata, P. abies x P. obovata) seedlings originating from different provenances in Russia were studied in the open-field ozone fumigation system located in Kuopio, Finland, over a span of 2 y. The AOT40 value (accumulated ozone dose over the threshold 40 ppb during daylight hours) was 11 ppm hr per growing season, which was 1.4 times higher than the ambient air concentration. The plants were measured for germination rate; shoot increment; needle length; and dry mass of needles, shoots, and roots. Significant differences between pine and spruce provenance response to ozone were found in all parameters. Ozone stress immediately reduced the germination rate of Northern pine provenances, whereas biomass reductions became evident during the second year of the exposure in all pine provenances. Spruce species were more tolerant to elevated ozone concentrations. Our results indicate that seedling development is vulnerable to increasing ozone concentrations and that attention must be paid to the provenance selection.

  10. Differential introgression reveals candidate genes for selection across a spruce (Picea sitchensis × P. glauca) hybrid zone.

    PubMed

    Hamilton, Jill A; Lexer, Christian; Aitken, Sally N

    2013-02-01

    Differential patterns of introgression between species across ecological gradients provide a fine-scale depiction of extrinsic and intrinsic factors that contribute to the maintenance of species barriers and adaptation across heterogeneous environments. Introgression was examined for 721 individuals collected from the ecological transition zone spanning maritime to continental climates within the Picea sitchensis-Picea glauca contact zone using a panel of 268 candidate gene single nucleotide polymorphisms. Geographic clines showed a strong spatial relationship between allele frequencies and both distance from the ocean along major rivers and mean annual precipitation, indicating a strong role for environmental selection. Interspecific patterns of differentiation using outlier tests revealed three candidate genes that may be targets of long-term divergent selection between the parental species, although contemporary genomic clines within the hybrid zone suggested neutral patterns of introgression for these genes. This study provides a fine-scale analysis of locus-specific introgression, identifying a suite of candidate loci that may be targets of extrinsic or intrinsic selection, with broad application in understanding local adaptation to climate.

  11. Overexpression of Laccaria bicolor aquaporin JQ585595 alters root water transport properties in ectomycorrhizal white spruce (Picea glauca) seedlings.

    PubMed

    Xu, Hao; Kemppainen, Minna; El Kayal, Walid; Lee, Seong Hee; Pardo, Alejandro G; Cooke, Janice E K; Zwiazek, Janusz J

    2015-01-01

    The contribution of hyphae to water transport in ectomycorrhizal (ECM) white spruce (Picea glauca) seedlings was examined by altering expression of a major water-transporting aquaporin in Laccaria bicolor. Picea glauca was inoculated with wild-type (WT), mock transgenic or L. bicolor aquaporin JQ585595-overexpressing (OE) strains and exposed to root temperatures ranging from 5 to 20°C to examine the root water transport properties, physiological responses and plasma membrane intrinsic protein (PIP) expression in colonized plants. Mycorrhization increased shoot water potential, transpiration, net photosynthetic rates, root hydraulic conductivity and root cortical cell hydraulic conductivity in seedlings. At 20°C, OE plants had higher root hydraulic conductivity compared with WT plants and the increases were accompanied by higher expression of P. glauca PIP GQ03401_M18.1 in roots. In contrast to WT L. bicolor, the effects of OE fungi on root and root cortical cell hydraulic conductivities were abolished at 10 and 5°C in the absence of major changes in the examined transcript levels of P. glauca root PIPs. The results provide evidence for the importance of fungal aquaporins in root water transport of mycorrhizal plants. They also demonstrate links between hyphal water transport, root aquaporin expression and root water transport in ECM plants.

  12. Ozone fumigation under dark/light conditions of Norway Spruce (Picea Abies) and Scots Pine (Pinus Sylvestris)

    NASA Astrophysics Data System (ADS)

    Canaval, Eva; Jud, Werner; Hansel, Armin

    2015-04-01

    Norway Spruce (Picea abies) and Scots Pine (Pinus sylvestris) represent dominating tree species in the northern hemisphere. Thus, the understanding of their ozone sensitivity in the light of the expected increasing ozone levels in the future is of great importance. In our experiments we investigated the emissions of volatile organic compounds (VOCs) of 3-4 year old Norway Spruce and Scots Pine seedlings under ozone fumigation (50-150 ppbv) and dark/light conditions. For the experiments the plants were placed in a setup with inert materials including a glass cuvette equipped with a turbulent air inlet and sensors for monitoring a large range of meteorological parameters. Typical conditions were 20-25°C and a relative humidity of 70-90 % for both plant species. A fast gas exchange rate was used to minimize reactions of ozone in the gas phase. A Switchable-Reagent-Ion-Time-of-Flight-MS (SRI-ToF-MS) was used to analyze the VOCs at the cuvette outlet in real-time during changing ozone and light levels. The use of H3O+ and NO+ as reagent ions allows the separation of certain isomers (e.g. aldehydes and ketones) due to different reaction pathways depending on the functional groups of the molecules. Within the Picea abies experiments the ozone loss, defined as the difference of the ozone concentration between cuvette inlet and outlet, remained nearly constant at the transition from dark to light. This indicates that a major part of the supplied ozone is depleted non-stomatally. In contrast the ozone loss increased by 50 % at the transition from dark to light conditions within Pinus sylvestris experiments. In this case the stomata represent the dominant loss channel. Since maximally 0.1% of the ozone loss could be explained by gas phase reactions with monoterpenes and sesquiterpenes, we suggest that ozone reactions on the surface of Picea abies represent the major sink in this case and lead to an light-independent ozone loss. This is supported by the fact that we detected

  13. Current land bird distribution and trends in population abundance between 1982 and 2012 on Rota, Mariana Islands

    USGS Publications Warehouse

    Camp, Richard J.; Brinck, Kevin W.; Gorresen, P. Marcos; Amidon, Fred A.; Radley, Paul M.; Berkowitz, S. Paul; Banko, Paul C.

    2015-01-01

    The western Pacific island of Rota is the fourth largest human-inhabited island in the Mariana archipelago and designated an Endemic Bird Area. Between 1982 and 2012, 12 point-transect distance-sampling surveys were conducted to assess bird population status. Surveys did not consistently sample the entire island; thus, we used a ratio estimator to estimate bird abundances in strata not sampled during every survey. Trends in population size were reliably estimated for 11 of 13 bird species, and 7 species declined over the 30-y time series, including the island collared-dove Streptopelia bitorquata, white-throated ground-dove Gallicolumba xanthonura, Mariana fruit-dove Ptilinopus roseicapilla, collared kingfisher Todiramphus chloris orii, Micronesian myzomela Myzomela rubratra, black drongo Dicrurus macrocercus, and Mariana crow Corvus kubaryi. The endangered Mariana crow (x̄  =  81 birds, 95% CI 30–202) declined sharply to fewer than 200 individuals in 2012, down from 1,491 birds in 1982 (95% CI  =  815–3,115). Trends increased for white tern Gygis alba, rufous fantail Rhipidura rufifrons mariae, and Micronesian starling Aplonis opaca. Numbers of the endangered Rota white-eye Zosterops rotensis declined from 1982 to the late 1990s but returned to 1980s levels by 2012, resulting in an overall stable trend. Trends for the yellow bittern Ixobrychus sinensis were inconclusive. Eurasian tree sparrow Passer montanus trends were not assessed; however, their numbers in 1982 and 2012 were similar. Occupancy models of the 2012 survey data revealed general patterns of land cover use and detectability among 12 species that could be reliably modeled. Occupancy was not assessed for the Eurasian tree sparrow because of insufficient detections. Based on the 2012 survey, bird distribution and abundance across Rota revealed three general patterns: 1) range restriction, including Mariana crow, Rota white-eye, and Eurasian tree sparrow; 2) widespread distribution, low

  14. Chemical composition of hydrothermal fluids in the central and southern Mariana Trough backarc basin

    NASA Astrophysics Data System (ADS)

    Ishibashi, Jun-ichiro; Tsunogai, Urumu; Toki, Tomohiro; Ebina, Naoya; Gamo, Toshitaka; Sano, Yuji; Masuda, Harue; Chiba, Hitoshi

    2015-11-01

    We present a geochemical data set from hydrothermal fluids collected from the central and southern Mariana Trough during dive programs conducted in the 1990s. The fluid samples were collected from two hydrothermal fields: (1) the Alice Springs field (18°13‧N, 144°42‧E, water depth: 3600 m) on the axial ridge crest of the central Marana Trough; and (2) the Forecast field (13°24‧N, 143°55‧E, water depth: 1470 m) on Peak B seamount, one of a cluster of small volcanic seamounts which represents a unique tectonic setting in the southern Mariana Trough. Gas geochemistry of the Alice Springs fluid showed He and C isotope ratios in the range of MORB, whereas that of the Forecast fluid showed a higher CO2/3He ratio and 13C-enriched isotope ratio, both of which are commonly recognized in hydrothermal fluids from intraoceanic arc volcanoes. The major and minor element composition of the Alice Springs fluid is characterized by enrichment in mobile alkali elements such as K, Rb and Cs. This geochemical signature is in accordance with the well-known characteristics of basaltic lava collected from the same area, which show substantial enrichment in incompatible elements. As interpreted in previous petrological studies, this signature is considered to be an arc geochemical signal reflecting contribution from the subduction component. The B concentration of the Alice Springs fluid is also high and is another arc geochemical signal. The Forecast fluid, by contrast, did not show enrichment in the mobile alkali elements, although its B concentration is high. The Forecast fluid is characterized instead by enrichment in Ca and Sr coupled with depletion in Na and Li. As discussed in previous studies, this signature is attributed to hydrothermal reactions at rather low temperature due to the shallow water depth. We recognize two different arc geochemical signals in these two hydrothermal fluids: mobile alkali elements in the Alice Spring fluid and gaseous species in the

  15. Sediment and Rock Samples Recovered from the Challenger Deep, Southern Mariana Trench

    NASA Astrophysics Data System (ADS)

    Fryer, P. B.

    2013-12-01

    Sediments collected with push cores during Nereus hybrid-ROV traverses within the trench axis of the Challenger Deep were squeezed onboard ship (R/V Kilo Moana) to extract pore fluids. The squeeze-cakes were analyzed by XRD, SEM, Raman spectroscopy and electron microprobe. The analysis reveals an assemblage of clays, and volcanic ash that contains plagioclase, clino-pyroxene, opaques and, glass. Chlorite is present as are hydrated iron oxides and fragments of diatoms. The sediment is predominantly very fine-grained and video from both the Nereus traverses across the Challenger Deep in 2009 and from the Deepsea Challenger submersible dive by James Cameron in 2012 indicate recent resurfacing of the trench axis. This is consistent with the high degree of deformation and frequency of earthquakes in the southern Mariana forearc north of the trench. An apron of self-derived talus blankets the lower part of the inner trench slope and fines from submarine landslides are the likely source of the trench axis sediment. Rock samples collected using Nereus from deep (10,879 m) on the incoming plate south of the Challenger Deep are partially altered microgabbros with interstitial glass containing microtubules similar to those observed in a variety of marine settings in lavas and hypabyssal igneous rocks. The tubules are presumed to be caused by tunneling of lithoautotrophic microbes into the glass. The rocks were collected from a site at the base of a fault scarp where large columnar-jointed blocks are draped with sediment. The igneous basement of the subducting plate south of the Challenger Deep is, as yet, undated, but it may be younger than the Jurassic Pacific Plate subducting beneath the southeastern Mariana forearc. There is the suggestion of a boundary between and the Pacific Plate and the shallower sea floor (Caroline plate?) subducing beneath the southernmost arm of the Mariana Trench. Early interpretations by Hegarty and Weissel (1988) and more recently by Lee (2004

  16. First results from TN273 studies of the SE Mariana Forearc rift

    NASA Astrophysics Data System (ADS)

    Ribeiro, J. M.; Stern, R. J.; Kelley, K. A.; Shaw, A. M.; Shimizu, N.; Martinez, F.; Ishii, T.; Ishizuka, O.; Manton, W. I.

    2012-12-01

    TN 273 aboard R/V Thomas Thompson (Dec. 22 2011- Jan. 22 2012) studied an unusual region of rifting affecting the southern Mariana forearc S.W. of Guam. The S.E. Mariana Forearc Rift (SEMFR) formed by diffuse tectonic and volcanic deformation (Martinez and Sleeper, this meeting) ~2.7-3.7 Ma ago to accommodate opening of the southernmost Mariana Trough backarc basin. A total of 730 km linear-track of SEMFR seafloor was surveyed with deep-towed side-scan sonar IMI-30. 14 dredges provided samples of SEMFR igneous rocks, analyzed for whole rock (WR) and glass compositions. These new results coupled with results of earlier investigations confirm that SEMFR is dominated by Miocene lavas along with minor gabbro and diabase. SEMFR lavas range in major element composition from primitive basalt to fractionated andesite (Mg# = 0.36-0.73; SiO2 = 50-57 wt%), mainly controlled by crystal fractionation. Rare Earth Element (REE) patterns range from LREE-depleted, N-MORB-like to flat patterns, reflecting different mantle processes (i.e. different sources, degree of melting …). Glassy rinds and olivine-hosted melt inclusions in these lavas contain variable volatile compositions (F = 75-358 ppm, S = 35-1126 ppm, Cl= 74-1400 ppm, CO2 = 15-520 ppm, 0.36-2.36 wt% H2O). SEMFR lavas show spider diagrams with positive anomalies in LILE and negative anomalies in HSFE. SEMFR lavas have backarc basin-like (BAB-like) chemical composition (H2O < 2.5wt%, Ba/Yb~20, Nb/Yb~1 and ɛNd~9) along with stronger enrichment in Rb and Cs than arc and BAB lavas, as demonstrated by their higher Rb/Th and Cs/Ba ratios in WR and glasses, which may reflect the role of the ultra-shallow fluids. Ultra-shallow fluids are derived from the top of the subducting slab, beneath the forearc, where most of the water and the fluid-mobile elements (Rb, Cs, Ba,) are thought to be released (Schmidt and Poli, 1998, EPSL, Savov et al., 2005, G-3). Our results suggest that i) SEMFR lavas formed by metasomatism of a BAB mantle

  17. Electrical resistivity structure of the upper mantle in the Southern Mariana Trough

    NASA Astrophysics Data System (ADS)

    Shindo, H.; Seama, N.; Matsuno, T.; Shibata, Y.; Kimura, M.; Nogi, Y.; Okino, K.

    2012-12-01

    We show a 2-D electrical resistivity structure of the upper mantle in the Southern Mariana Trough. The Mariana Trough is an active back-arc basin in which the seafloor spreading has occurred. In the southern region of the Mariana Trough, the seafloor spreading rate is 35-45 km/Myr at present (Kato et al., 2003) that is slow, but there are characteristics of the fast spreading ridge such as an axial topographic high (Martinez et al., 2000) and a nearly constant low mantle Bouguer anomaly along the spreading axis suggesting high magmatic activity with a sheet-like mantle upwelling under the spreading axis (Kitada et al., 2006). We carried out an electromagnetic experiment along a ~120 km length profile across the spreading axis to estimate an electrical resistivity structure, and hence the physical property like temperature, water and melt content in the upper mantle. The observation was made using ten Ocean Bottom Electro-Magnetometers (OBEMs) from August to November in 2010. The data was recorded for ~85 days in two OBEMs and for ~60 days in the rest of the OBEMs. Successfully, eight OBEMs recorded time-variations of the electric and magnetic fields and two OBEMs recorded only those of the magnetic field. The magnetotelluric (MT) method is a base for the data analysis. We carried out the time-series data analysis to estimate the MT responses and correct topographic distortions in the MT responses. We have basically performed a smooth model inversion analysis using the processed MT responses to estimate a minimum electrical resistivity structure, and also have considered a prior constraint in the inversion analysis for the subducted slab inferred from a seismic research (Gudmundsson and Sambridge, 1998). The obtained 2-D electrical resistivity structure shows an asymmetry about the spreading center. The trenchward side shows higher resistivity (~300 Ohm-m), while the opposite side (the west side) shows that intermediate resistivity (~100 Ohm-m) with ~40 km thickness

  18. Crustal evolution derived from the Izu-Bonin-Mariana arc velocity images

    NASA Astrophysics Data System (ADS)

    Takahashi, N.; Kodaira, S.; Tatsumi, Y.; Miura, S.; Sato, T.; Yamashita, M.; No, T.; Takahashi, T.; Noguchi, N.; Takizawa, K.; Kaiho, Y.; Kaneda, Y.

    2010-12-01

    The Izu-Bonin-Mariana arc is known as one of typical oceanic island arcs, which has developed by subduction between oceanic crusts producing continental materials. Japan Agency for Marine-Earth Science and Technology has carried out seismic surveys using a multi-channel reflection survey system (MCS) and ocean bottom seismographs (OBSs) in the Izu-Bonin-Mariana (IBM) arc since 2002, and reported these crustal images. As the results, we identified the structural characteristics of whole Izu-Bonin-Mariana arc. Rough structural characteristics are, 1) middle crust with Vp of 6 km/s, 2) upper part of the lower crust with Vp of 6.5-6.8 km/s, 3) lower part of the lower crust with Vp of 6.8-7.5 km/s, and 4) lower mantle velocity beneath the arc crusts. In addition, structural variation along the volcanic front, for example, thickness variation of andesitic layers was imaged and the distributions is consistent with those of rhyolite volcanoes, that is, it suggested that the cause the structural variation is various degree of crustal growth (Kodaira et al., 2007). Moreover, crustal thinning with high velocity lower crust across arc was also imaged, and it is interpreted that such crust has been influenced backarc opening (Takahashi et al., 2009). According to Tatsumi et al. (2008), andesitic middle crust is produced by differentiation of basaltic lower crust and a part of the restites are transformed to the upper mantle. This means that region showing much crustal differentiation has large volume of transformation of dense crustal materials to the mantle. We calculated volume profiles of the lower crust along all seismic lines based on the petrologic model, and compared them with observed real volumes obtained by seismic images. If the real volume of the lower crust is large, it means that the underplating of dense materials to the crustal bottom is dominant rather than transformation of dense materials to the upper mantle. According to obtained profiles to judge if the

  19. Hydroacoustic Records of the First Historical Eruption of Anatahan Volcano, Mariana Islands

    NASA Astrophysics Data System (ADS)

    Dziak, R.; Park, M.; Matsumoto, H.; Fox, C.; Byun, S.; Fowler, M.; Haxel, J.; Embley, R.

    2003-12-01

    For the past decade, NOAA/Pacific Marine Environmental Laboratory has monitored volcano-seismic activity from western Pacific island-arc volcanoes using an array of U.S. Navy hydrophones (called SOSUS) deployed at fixed locations throughout the North Pacific Ocean. SOSUS hydrophones are mounted within the SOFAR channel and record the hydroacoustic tertiary phase or T-wave of oceanic earthquakes from throughout the Pacific basin. Since acoustic T-waves obey cylindrical energy attenuation as opposed to the spherical attenuation of solid-earth seismic phases, sound channel hydrophones can detect often smaller and therefore more numerous earthquakes than land-based seismic networks. This property allowed for the detection of harmonic tremor from a submarine volcano in the Volcano Islands on hydrophones >14,000 km away in the eastern Pacific. The first historical eruption of Anatahan Volcano appears to have started (from satellite imagery) at 1730Z on 10 May, with an ash plume visible by 2232Z (BGVN, 5 May 2003). Records from a broadband seismometer deployed on nearby ( ˜6.5 km) Sarigan Island indicate earthquake activity increased at about 1300Z on 10 May (D. Weins, pers com). SOSUS hydrophones in the western Pacific ( ˜4000 km distant) also recorded increased earthquake activity at 1300Z on 10 May as well as continuous, low-frequency (<10 Hz) energy (possible volcanic tremor) that began about a day before the seismicity. The earthquakes and tremor were detected on only two SOSUS hydrophones and therefore it was not possible to estimate their source location. The arrival azimuth of the signals were, however, consistent with a source in the Mariana Islands. To complement the SOSUS hydrophone array coverage in the western Pacific Ocean, an array of five autonomous hydrophones were deployed in February 2003 (sponsored by NOAA's Ocean Exploration Program) within the SOFAR channel along the active island- and back-arc of the Mariana Islands. All five hydrophones (1-110 Hz

  20. Application of game theory to the interface between militarization and environmental stewardship in the Mariana Islands.

    PubMed

    Marler, Thomas E; Wiecko, Greg; Moore, Aubrey

    2012-03-01

    We recently described threats to the terrestrial biological resources on the Mariana islands of Guam and Tinian resulting from the large-scale buildup of military operations. Attitudes that view these military buildup plans in a zero sum context whereby the positives of greater security and improved local economy can be attained only with corresponding negatives of environmental destruction are prevalent. We argue these attitudes oversimplify the complicated interactions between military operations and environmental damage. Here we discuss aspects of our case study that would benefit from application of game theory. Declines in ecosystem health are not unavoidable forms of collateral damage of peace-time military operations. We repeat, conservation of environmental resources is not ancillary to national security, it is integral.

  1. Diet composition of the invasive cane toad (Chaunus marinus) on Rota, Northern Mariana Islands

    USGS Publications Warehouse

    Reed, R.N.; Bakkegard, K.A.; Desy, G.E.; Plentovich, S.M.

    2007-01-01

    The cane or marine toad (Chaunus marinus, formerly Bufo marinus) was introduced to the Northern Mariana Islands starting in the 1930s. The effects of this exotic predator on native vertebrates (especially lizards) are largely unknown. We analysed the stomach contents of 336 cane toads collected from the island of Rota, with the goal of estimating the level of toad predation on native vertebrates. Beetles, ants, millipedes, and grasshoppers/crickets comprised the majority of prey classes consumed by toads. The introduced Brahminy blindsnake (Ramphotyphlops braminus; N = 6) and conspecific cane toads (N = 4) were the vertebrates most commonly found in toad stomachs. Skinks (Emoia; N = 2) were the only native vertebrates represented in our sample. The small numbers of nocturnal terrestrial vertebrates native to Rota likely translates to relatively low rates of predation by cane toads on native vertebrates.

  2. Population status of the Tinian Monarch (Monarcha takatsukasae) on Tinian, Commonwealth of the Northern Mariana Islands

    USGS Publications Warehouse

    Lusk, Michael; Hess, Steve; Reynolds, Michelle H.; Johnston, Scott

    2000-01-01

    We conducted surveys to evaluate the current population status of the Tinian Monarch (Monarcha takatsukasae), an insectivorous forest bird restricted to the island of Tinian, Commonwealth of the Northern Mariana Islands. In 1996, we recounted transects surveyed in 1982 and used the same analysis procedure to compare 1982 and 1996 population estimates. The 1996 population estimate was 55,721 ± 3,846 SE (48,345–63,495 95% CI), which is 57% higher than our estimate of 35,846 ± 2,211 SE (31,668–40,337 95% CI) for 1982. Vegetation density, measured by canopy cover and lateral visibility at each station, has also increased since 1982. Our data suggest that the Tinian Monarch population has increased possibly due to increased habitat availability, but remains at risk both from an accidental introduction of brown tree snakes (Boiga irregularis) to the island and stochastic processes.

  3. A Fluid Sea in the Mariana Islands: Community Archaeology and Mapping the Seascape of Saipan

    NASA Astrophysics Data System (ADS)

    McKinnon, Jennifer; Mushynsky, Julie; Cabrera, Genevieve

    2014-06-01

    This paper applies both a community archaeology and seascape approach to the investigation of the sea and its importance to the Indigenous community on the island of Saipan in the Mariana Islands in western Oceania. It examines data collected during a community project including archaeological sites, oral histories, lived experiences and contemporary understandings of both tangible and intangible maritime heritage to explore Indigenous connections with the sea and better define the seascape. What the seascape of Saipan conveys in the larger sense is the true fluidity of the sea. In this instance fluidity has more than one connotation; it refers to the sea as both a substance and an idea that permeates and flows into all aspects of Indigenous life. Chamorro and Carolinian people of Saipan identify themselves as having an ancestral connection with the sea that they continue to maintain to this day as they engage in daily activities within their seascape.

  4. Transcriptome profiling in conifers and the PiceaGenExpress database show patterns of diversification within gene families and interspecific conservation in vascular gene expression

    PubMed Central

    2012-01-01

    Background Conifers have very large genomes (13 to 30 Gigabases) that are mostly uncharacterized although extensive cDNA resources have recently become available. This report presents a global overview of transcriptome variation in a conifer tree and documents conservation and diversity of gene expression patterns among major vegetative tissues. Results An oligonucleotide microarray was developed from Picea glauca and P. sitchensis cDNA datasets. It represents 23,853 unique genes and was shown to be suitable for transcriptome profiling in several species. A comparison of secondary xylem and phelloderm tissues showed that preferential expression in these vascular tissues was highly conserved among Picea spp. RNA-Sequencing strongly confirmed tissue preferential expression and provided a robust validation of the microarray design. A small database of transcription profiles called PiceaGenExpress was developed from over 150 hybridizations spanning eight major tissue types. In total, transcripts were detected for 92% of the genes on the microarray, in at least one tissue. Non-annotated genes were predominantly expressed at low levels in fewer tissues than genes of known or predicted function. Diversity of expression within gene families may be rapidly assessed from PiceaGenExpress. In conifer trees, dehydrins and late embryogenesis abundant (LEA) osmotic regulation proteins occur in large gene families compared to angiosperms. Strong contrasts and low diversity was observed in the dehydrin family, while diverse patterns suggested a greater degree of diversification among LEAs. Conclusion Together, the oligonucleotide microarray and the PiceaGenExpress database represent the first resource of this kind for gymnosperm plants. The spruce transcriptome analysis reported here is expected to accelerate genetic studies in the large and important group comprised of conifer trees. PMID:22931377

  5. Electromagnetic constraints on a melt region beneath the central Mariana back-arc spreading ridge

    NASA Astrophysics Data System (ADS)

    Matsuno, Tetsuo; Evans, Rob L.; Seama, Nobukazu; Chave, Alan D.

    2012-10-01

    An electrical resistivity profile across the central Mariana subduction system shows high resistivity in the upper mantle beneath the back-arc spreading ridge where melt might be expected to exist. Although seismic data are equivocal on the extent of a possible melt region, the question arises as to why a 2-D magnetotelluric (MT) survey apparently failed to image any melt. We have run forward models and inversions that test possible 3-D melt geometries that are consistent with the MT data and results of other studies from the region, and that we use to place upper bounds on the possible extent of 3-D melt region beneath the spreading center. Our study suggests that the largest melt region that was not directly imaged by the 2-D MT data, but that is compatible with the observations as well as the likely effects of melt focusing, has a 3-D shape on a ridge-segment scale focused toward the spreading center and a resistivity of 100 Ω-m that corresponds to ˜0.1-˜1% interconnected silicate melt embedded in a background resistivity of ˜500 Ω-m. In contrast to the superfast spreading southern East Pacific Rise, the 3-D melt region suggests that buoyant mantle upwelling on a ridge-segment scale is the dominant process beneath the slow-spreading central Mariana back-arc. A final test considers whether the inability to image a 3-D melt region was a result of the 2-D survey geometry. The result reveals that the 2-D transect completed is useful to elucidate a broad range of 3-D melt bodies.

  6. Rapid time scales of basalt to andesite differentiation at Anatahan volcano, Mariana Islands

    NASA Astrophysics Data System (ADS)

    Reagan, Mark; Tepley, Frank J.; Gill, James B.; Wortel, Matthew; Hartman, Brian

    2005-08-01

    We present comprehensive U-series data ( 238U- 234U- 230Th- 226Ra- 210Pb- 210Po and ( 230Th)/( 232Th)) for an andesite from an oceanic arc. The juvenile Anatahan andesite has U-Th systematics colinear with other historical Mariana volcanic rocks, and is most similar to those of the other volcano in the Mariana arc with a significant proportion of silicic andesite: Uracas. Like Uracas, the parental basalt for the Anatahan andesite was generated by relatively low degrees of flux melting from a source previously enriched in a sediment component from the subducting slab. However, the Anatahan andesite is much more strongly enriched in 226Ra over 230Th than Uracas lavas, and has one of the highest ( 226Ra)/( 232Th) ratios of siliceous andesites globally. The long-lived disequilibria between 238U- 230Th- 226Ra in the Anatahan andesite are inherited from basalt genesis, not created during differentiation or eruption. Thus, the time between genesis of the parental basalt and eruption of andesite at Anatahan is shorter than for Uracas. Moreover, the near-equilibrium ( 210Pb)/( 226Ra) value indicates that the magma body did not persistently lose or gain 222Rn for more than 2 years before eruption. This permits differentiation of the parental basalt to form andesite within this 2-year time period, although a differentiation time period between 100 and a few thousand years also is possible. The relative activities between 210Po and 210Pb suggest erupted scoria degassed Po less than most lavas despite eruption plume heights of ˜10 km, which further suggests an unusually rapid ascent before eruption. These data also show that juvenile material was ejected from the first day of the eruption. Phreatomagmatic ejecta overlying the main Anatahan scoria is strongly enriched in 210Po over 210Pb, indicating that a significant proportion of the Po degassed from rising magmas sublimes in its shallow fumarolic conduit system.

  7. Distribution, density, and biomass of introduced small mammals in the southern mariana islands

    USGS Publications Warehouse

    Wiewel, A.S.; Adams, A.A.Y.; Rodda, G.H.

    2009-01-01

    Although it is generally accepted that introduced small mammals have detrimental effects on island ecology, our understanding of these effects is frequently limited by incomplete knowledge of small mammal distribution, density, and biomass. Such information is especially critical in the Mariana Islands, where small mammal density is inversely related to effectiveness of Brown Tree Snake (Boiga irregularis) control tools, such as mouse-attractant traps. We used mark-recapture sampling to determine introduced small mammal distribution, density, and biomass in the major habitats of Guam, Rota, Saipan, and Tinian, including grassland, Leucaena forest, and native limestone forest. Of the five species captured, Rattus diardii (sensu Robins et al. 2007) was most common across habitats and islands. In contrast, Mus musculus was rarely captured at forested sites, Suncus murinus was not captured on Rota, and R. exulans and R. norvegicus captures were uncommon. Modeling indicated that neophobia, island, sex, reproductive status, and rain amount influenced R. diardii capture probability, whereas time, island, and capture heterogeneity influenced S. murinus and M. musculus capture probability. Density and biomass were much greater on Rota, Saipan, and Tinian than on Guam, most likely a result of Brown Tree Snake predation pressure on the latter island. Rattus diardii and M. musculus density and biomass were greatest in grassland, whereas S. murinus density and biomass were greatest in Leucaena forest. The high densities documented during this research suggest that introduced small mammals (especially R. diardii) are impacting abundance and diversity of the native fauna and flora of the Mariana Islands. Further, Brown Tree Snake control and management tools that rely on mouse attractants will be less effective on Rota, Saipan, and Tinian than on Guam. If the Brown Tree Snake becomes established on these islands, high-density introduced small mammal populations will likely

  8. De-facto marine protection from a Navy bombing range: Farallon De Medinilla, Mariana Archipelago, 1997 to 2012.

    PubMed

    Smith, Stephen H; Marx, Donald E

    2016-01-15

    Fourteen surveys were conducted at Farallon De Medinilla (a U.S. Department of Defense bombing range in the Mariana Archipelago) between 1997 and 2012; annual surveys were conducted from 1999 through 2012. There was no evidence that the condition of the biological resources assessed had changed, or been adversely impacted to a significant degree by the training activities being conducted there. Restricted access has resulted in a de-facto preserve effect and outweighs minor negative impacts from training. The health, abundance and biomass of fishes, corals and other marine resources are comparable to or superior to those in similar habitats at other locations within the Mariana Archipelago. Our research suggests that the greatest threat to FDM's marine resources is from fishermen, not military training activities.

  9. Epidihydropinidine, the main piperidine alkaloid compound of Norway spruce (Picea abies) shows promising antibacterial and anti-Candida activity.

    PubMed

    Fyhrquist, Pia; Virjamo, Virpi; Hiltunen, Eveliina; Julkunen-Tiitto, Riitta

    2017-03-01

    This study reports for the first time promising antibacterial and antifungal effects of epidihydropinidine, the major piperidine alkaloid in the needles and bark of Norway spruce, Picea abies (L.) Karsten. Epidihydropinidine was growth inhibitory against all bacterial and fungal strains used in our investigation, showing the lowest MIC value of 5.37μg/mL against Pseudomonas aeruginosa, Enterococcus faecalis, Candida glabrata and C. albicans. Epidihydropinidine was nearly three times more active than tetracycline against P. aeruginosa and E. faecalis. Promising antibacterial effects were also recorded against Staphylococcus aureus and Bacillus cereus (MIC 10.75μg/mL) as well as against Salmonella enterica (MIC and MBC 43μg/mL). Our preliminary results suggest that epidihydropinidine as well related alkaloids of Norway spruce could be powerful candidates for new antibiotics and for preventing food spoilage.

  10. [Cytogenetic abnormalities in seedlings of Norway spruce (Picea abies (L.) Karst.) from the natural populations and introduction plantation].

    PubMed

    Korshikov, I I; Tkacheva, Iu A; Privalikhin, S N

    2012-01-01

    Comparative studies of the frequency and spectrum of pathological mitosis (PM), as well as of nucleoli number in the interphase cells of seedling roots have been performed in two natural populations of Norway spruce (Picea abies (L.) Karst.) of Ukrainian Polesie and introduction plantation in the Donbass. Low levels of PM in the seed progeny populations (0.32-0.38%) and slightly higher in the progeny plantation (0.40%) have been installed. Number of nucleoli was slightly higher in the plants of natural populations (5.35-5.85) than that of the plantation (4.95). The frequency of PM in the offspring of low-heterozygous plants was higher (0.43%) than in highly heterozygous individuals (0.28%).

  11. Spring temperatures in the far-western Nepal Himalaya since AD 1640 reconstructed from Picea smithiana tree-ring widths

    NASA Astrophysics Data System (ADS)

    Thapa, Udya Kuwar; Shah, Santosh K.; Gaire, Narayan Prasad; Bhuju, Dinesh Raj

    2015-10-01

    We developed a new, 422-year long tree-ring width chronology (spanning AD 1591-2012) from Picea smithiana (Wall.) Boiss in Khaptad National Park, which is located in the far-western Nepalese Himalaya. Seasonal correlation analysis revealed significant indirect relationship with spring temperature and lead to the reconstruction of March-May average temperature for the past 373 years (AD 1640-2012). The reconstruction was found significant based on validation statistics commonly used in tree-ring based climate reconstruction. Furthermore, it was validated through spatial correlation with gridded temperature data. This temperature reconstruction identified several periods of warming and cooling. The reconstruction did not show the significant pattern of cooling during the Little Ice Age but there were few cold episodes recorded. The spring temperature revealed relationship with different Sea Surface Temperature index over the equatorial Pacific Ocean, which showed linkages with climatic variability in a global scale.

  12. Tinian Island Nas, Mariana Islands. Revised Uniform Summary of Surface Weather Observations (RUSSWO). Parts A-F.

    DTIC Science & Technology

    1973-05-25

    unlimited distribution of this report to the public at large, or by DDC to the National Technical Information Service (NTIS). a technical report has be...OBSOLETE DATA PROCESSING RRANCH ETAC/USAF SURFACE WINDS 2 AIR WEATER SERVICE /MAC PERCENTAGE FREQUENCY OF WIND DIRECTION AND SPEED (FROM HOURLY... Service ( MAC ) REVISED UNIFORM SUMMARY OF SURF6CE WEATHER OBSERVATION5 TINIAN NAS MARIANA IS WBAN #41413 N 150 0 E 145 38 ELEV 250 FT WMO# PARTS A-F

  13. Online Classroom Research and Analysis Activities Using MARGINS-Related Resources for the Izu-Bonin-Mariana Subduction System

    NASA Astrophysics Data System (ADS)

    Ryan, J. G.

    2007-12-01

    Students today have online access to nearly unlimited scientific information in an entirely unfiltered state. As such, they need guidance and training in identifying and assessing high-quality information resources for educational and research use. The extensive research data resources available online for the Izu-Bonin-Mariana (IBM) subduction system that have been developed with MARGINS Program and related NSF funding are an ideal venue for focused Web research exercises that can be tailored to a range of undergraduate geoscience courses. This presentation highlights student web research activities examining: a) The 2003-2005 eruptions of Anatahan Volcano in the Mariana volcanic arc. MARGINS-supported geophysical research teams were in the region when the eruption initiated, permitting a unique "event response" data collection and analysis process, with preliminary results presented online at websites linked to the MARGINS homepage, and ultimately published in a special issue of the Journal of Volcanology and Geothermal Research. In this activity, students will conduct a directed Web surf/search effort for information on and datasets from the Anatahan arc volcano, which they will use in an interpretive study of recent magmatic activity in the Mariana arc. This activity is designed as a homework exercise for use in a junior-senior level Petrology course, but could easily be taken into greater depth for the benefit of graduate-level volcanology or geochemistry offerings. b) Geochemical and mineralogical results from ODP Legs 125 and 195 focused on diapiric serpentinite mud volcanoes, which erupt cold, high pH fluids, serpentine muds, and serpentinized ultramafic clasts at a number of sites in the forearc region of the Mariana subduction zone. The focus of this activity is an examination of the trace element chemistry of the forearc serpentines and their associated upwelling porefluids as a means of understanding the roles of ionic radius, valence, and system

  14. Defense mechanisms against herbivory in Picea: sequence evolution and expression regulation of gene family members in the phenylpropanoid pathway

    PubMed Central

    2011-01-01

    Background In trees, a substantial amount of carbon is directed towards production of phenolics for development and defense. This metabolic pathway is also a major factor in resistance to insect pathogens in spruce. In such gene families, environmental stimuli may have an important effect on the evolutionary fate of duplicated genes, and different expression patterns may indicate functional diversification. Results Gene families in spruce (Picea) have expanded to superfamilies, including O-methyltransferases, cytochrome-P450, and dirigents/classIII-peroxidases. Neo-functionalization of superfamily members from different clades is reflected in expression diversification. Genetical genomics can provide new insights into the genetic basis and evolution of insect resistance in plants. Adopting this approach, we merged genotype data (252 SNPs in a segregating pedigree), gene expression levels (for 428 phenylpropanoid-related genes) and measures of susceptibility to Pissodes stobi, using a partial-diallel crossing-design with white spruce (Picea glauca). Thirty-eight expressed phenylpropanoid-related genes co-segregated with weevil susceptibility, indicating either causative or reactive effects of these genes to weevil resistance. We identified eight regulatory genomic regions with extensive overlap of quantitative trait loci from susceptibility and growth phenotypes (pQTLs) and expression QTL (eQTL) hotspots. In particular, SNPs within two different CCoAOMT loci regulate phenotypic variation from a common set of 24 genes and three resistance traits. Conclusions Pest resistance was associated with individual candidate genes as well as with trans-regulatory hotspots along the spruce genome. Our results showed that specific genes within the phenylpropanoid pathway have been duplicated and diversified in the conifer in a process fundamentally different from short-lived angiosperm species. These findings add to the information about the role of the phenylpropanoid pathway in

  15. Present status of Maconellicoccus hirsutus (Hemiptera: Pseudococcidae) in the Mariana Islands and its control by two fortuitously introduced natural enemies.

    PubMed

    Reddy, G V P; Muniappan, R; Cruz, Z T; Naz, F; Bamba, J P; Tenorio, J

    2009-08-01

    The mealybug Maconellicoccus hirsutus (Green) (Hemiptera: Pseudococcidae), attacks ornamental and fruit crops in the Mariana Islands. Insecticides cannot penetrate the heavy layers of wax that protect the insect's body. We surveyed the mealybug's locally recruited natural enemies and their effects on its population on Guam, Rota, Saipan, and Tinian to assess the need for introduction of exotic natural enemies. We monitored population densities of M. hirsutus, those of its natural enemies, and parasitism rates for 3 yr, 2005-2007. Our surveys revealed the presence of two parasitoids, Anagyrus kamali Moursi (Hymenoptera: Encyrtidae) and Allotropa sp. near mecrida (Walker) (Hymenoptera: Platygastridae), fortuitously introduced to the Mariana Islands with M. hirsutus. The predator Cryptolaemus montrouzieri Mulsant (Coleoptera: Coccinellidae) also was often found feeding on M. hirsutus. Population density of M. hirsutus was below the economic threshold at all locations. Rainfall seemed to affect mean numbers of M. hirsutus and mean numbers of eggs at some locations. On all four islands, the two parasitoids, complemented by the predator, were effectively controlling the M. hirsutus population. No evidence of hyperparasitism was recorded. Currently, economic damage by M. hirsutus is not a concern in the Mariana Islands, and additional parasitoids need not be introduced to control M. hirsutus.

  16. Impact of phase change kinetics on the Mariana slab within the framework of 2-D mantle convection

    NASA Astrophysics Data System (ADS)

    Yoshioka, Shoichi; Torii, Yoku; Riedel, Michael R.

    2015-03-01

    Recent high-pressure and high-temperature experiments indicate that metastable olivine might persist in the cold core of a slab due to the low reaction rate of the olivine-wadsleyite phase transformation. Recent seismological observations detected a metastable olivine wedge that survives to a depth of 630 km in the Mariana slab. To consider the problem of non-equilibrium phase transformation, we developed a two-dimensional (2-D) Cartesian numerical code that incorporates the effects of kinetics into a thermal convection model. We consider the kinetics of the 410-km olivine-wadsleyite and the 660-km ringwoodite-Pv + Mw phase transformations, including the effects of water content at the 410-km phase boundary. The latent heat release of the 410-km non-equilibrium phase transformations inside the slab is also considered. The results show positive correlations between some of the controlling parameters and the length of the metastable olivine wedge: the faster the subducting velocity, and the lower the water content, the deeper is the metastable olivine wedge. With increasing depth of phase transformation, the effect of latent heat release is enhanced: heating of, at most, 100 °C occurs if olivine transforms into wadsleyite at a depth of approximately 570 km in our model setting. Temperature increase due to the latent heat released stimulates further phase transformation, resulting in further temperature increase, acting as a positive feedback effect. We also attempt to explain the seismological observations by calculating the temperature and phase structures in the Mariana slab. If we assume that the age of the Mariana slab is 150 Myr, the subduction velocity is 9.5 cm/yr, phase transformation occurs from the grain boundary of the parental phase, and the water content is 250 wt. ppm for a grain size of 1 mm, 300 wt. ppm for one of 5 mm, and 100 wt. ppm for intracrystalline transformation, then the metastable olivine wedge survives to a depth of 630 km, which is in

  17. Cycling of sulfur in subduction zones: The geochemistry of sulfur in the Mariana Island Arc and back-arc trough

    USGS Publications Warehouse

    Alt, J.C.; Shanks, Wayne C.; Jackson, M.C.

    1993-01-01

    The sulfur contents and sulfur isotopic compositions of 24 glassy submarine volcanics from the Mariana Island Arc and back-arc Mariana Trough were determined in order to investigate the hypothesis that subducted seawater sulfur (??34S = 21???) is recycled through arc volcanism. Our results for sulfur are similar to those for subaerial arc volcanics: Mariana Arc glasses are enriched in 34S (??34S = up to 10.3???, mean = 3.8???) and depleted in S (20-290 ppm, mean = 100 ppm) relative to MORB (850 ppm S, ??34S = 0.1 ?? 0.5???). The back-arc trough basalts contain 200-930 ppm S and have ??34S values of 1.1 ?? 0.5???, which overlap those for the arc and MORB. The low sulfur contents of the arc and some of the trough glasses are attributed to (1) early loss of small amounts of sulfur through separation of immiscible sulfide and (2) later vapor-melt equilibrium control of sulfur contents and loss of sulfur in a vapor phase from sulfide-undersaturated melts near the minimum in sulfur solubility at f{hook}O2 ??? NNO (nickel-nickel oxide). Although these processes removed sulfur from the melts their effects on the sulfur isotopic compositions of the melts were minimal. Positive trends of ??34S with 87Sr 86Sr, LILE and LREE contents of the arc volcanics are consistent with a metasomatic seawater sulfur component in the depleted sub-arc mantle source. The lack of a 34S-rich slab signature in the trough lavas may be attributed to equilibration of metasomatic fluid with mantle material along the longer pathway from the slab to the source of the trough volcanics. Sulfur is likely to have been transported into the mantle wedge by metasomatic fluid derived from subducted sediments and pore fluids. Gases extracted from vesicles in arc and back-arc samples are predominantly H2O, with minor CO2 and traces of H2S and SO2. CO2 in the arc and back-arc rocks has ??13C values of -2.1 to -13.1???, similar to MORB. These data suggest that degassing of CO2 could explain the slightly lower

  18. Methane Distribution In Plumes Of The South Mariana Back-arc Spreading Center

    NASA Astrophysics Data System (ADS)

    Toki, T.; Hirota, A.; Tsunogai, U.; Gamo, T.; Nakamura, K.; Noguchi, T.; Taira, N.; Oomori, T.; Ishibashi, J.; Utsumi, M.

    2004-12-01

    In the South Mariana Back-arc Spreading Center, two methane plumes were observed in water column based on analysis of methane in seawater samples collected during the R/V Thompson expeditions in 2003 around water depth of 2,700 m over the Fryer site on the ridge-axis seamount (12\\deg57.22N, 143\\deg37.16E, depth: 2,850 m). The estimated end-member isotopic compositions of methane in the two plumes are \\delta13C_{CH4} = -5‰ PDB and -50‰ PDB. These values indicated that the two plumes were originated from the different sources. During YK03-09 cruise using the submersible Shinkai 6500 from October to November in 2003, detailed seafloor observation discovered sulfide chimneys emitting black and clear hydrothermal fluid on the off-axis seamount at Pika site (12°55.15N, 143°36.96E, depth: 2,773 m). The result of analysis of isotopic composition of methane in the hydrothermal fluids recovered from the off-axis hydrothermal vents using WHATS (Water and Hydrothermal Atsuryoku Tight Sampler) was averaged value of -4‰ PDB (standard deviation = 1‰ PDB, n = 3). Hydrothermal fluids from the Fryer site were also sampled and were measured: average value = -6.7‰ PDB, standard deviation = 0.3‰ PDB, n = 3. During the R/V Thompson expeditions in March 2004 using ROV ROPOS, 11 ROPOS dives and CTD-RMS plume surveys were conducted, and newly discovered a huge hydrothermal structure with active fluid venting at Achaean site on the ridge skirt (12°56.37N, 143°37.92E, depth: 2,990 m). The δ ^{13}C_{CH4} value of the fluid sample from the site using ROCS (Rotary Clean Seawater sampler) was -14.7‰ PDB. Analysis of isotopic composition of methane in the plume samples collected using the CTD-hydrocast at water depth of 2,500 m over the Archaean site showed -45‰ PDB. Source of methane (δ ^{13}C_{CH4} = -50‰ PDB), however, in the two plumes of the South Mariana Back-arc Spreading Center has been missing. The δ ^{13}C of methane cannot be considered in sediment

  19. New Mapping of Mariana Submarine Volcanoes with Sidescan and Multibeam Sonars

    NASA Astrophysics Data System (ADS)

    Embley, R. W.; Chadwick, W. W.; Baker, E. T.; Johnson, P. D.; Merle, S. G.; Ristau, S.

    2003-12-01

    An expedition in February/March 2003 on the R/V Thomas G. Thompson mapped more than 18,000 km2 with the towed MR1 sidescan sonar and almost 28,000 km2 with an EM300 hull-mounted multibeam system along the Mariana volcanic arc. The expedition was funded by NOAA's Office of Ocean Exploration (more on the expedition can be found at: http://oceanexplorer.noaa.gov/explorations/03fire/welcome.html). The MR1 sidescan surveys began at the northern end of a 2001 R/V Melville MR1 survey at 16§ N and extended to Nikko Volcano at 23\\deg 05'N. A portion of the southern back-arc spreading center and the arc volcanoes south of 16\\deg N were mapped using the EM300 system. Of 43 submarine arc volcanoes surveyed that have basal diameters of 10 km or greater, 17 have summit calderas or craters. Of these, however, only 5 have diameters more than 2 km. In an accompanying survey of hydrothermal activity along the arc, CTD casts and/or tows were conducted over more than 50 individual volcanoes. The 11 volcanoes with active hydrothermal systems found in the course of these surveys appear to be about equally divided between those with and without summit calderas or craters (for additional information, see Baker et al., Resing et al., and Lupton et al., this session). The flanks of the submarine volcanoes and islands of the central and northern Mariana Arc consist largely of volcaniclastic flows. Most of the larger edifices have high-backscatter spoke-like patterns that probably represent coarser and/or younger flows from the summits. Higher relief high-backscatter areas, also commonly exhibiting a radial pattern, are found on many of the volcanoes' flanks. These are probably lava flows erupted along radial fissures. The Mariana Arc volcanoes are shedding large volumes of volcaniclastic material westward into the back-arc basin through a series of deep-sea channels oriented transverse to the arc that are in many places fed by flows from several volcanoes. On many of the volcaniclastic

  20. Submarine Arc Volcanism in the Southern Mariana Arc: Results of Recent ROV studies

    NASA Astrophysics Data System (ADS)

    Nichols, A. R.; Tamura, Y.; Stern, R. J.; Embley, R. W.; Hein, J. R.; Jordan, E.; Ribeiro, J. M.; Sica, N.; Kohut, E. J.; Whattam, S. A.; Hirahara, Y.; Senda, R.; Nunokawa, A.

    2009-12-01

    The submarine Diamante cross-arc volcanoes (~16°N) and the Sarigan-Zealandia Bank Multi-Volcano Complex (SZBMVC; ~16°45’N), north and south, respectively, of Anatahan Island in the southern Mariana Arc, were studied during several dives in June 2009 using the ROV Hyper-Dolphin, cruise NT09-08 (R/V Natsushima); neither has been studied in detail before. The data collected provide a new perspective on how the subduction factory operates to complement previous studies on other cross-arc volcanic chains in the Izu-Bonin-Mariana Arc. The Diamante complex consists of three major edifices, two cones (West and Central Diamante) and a more complex caldera-like edifice at the volcanic front (East Diamante). West and Central Diamante are basaltic volcanoes but East Diamante has a more complex history. Our studies indicate initial construction of a basaltic volcano. Magmatic evolution led to a violent caldera-forming and quieter dome-building events. Post-caldera quiescence allowed a carbonate platform to grow, now preserved on the eastern caldera wall. Felsic magma or hot rock provides a heat source for an active hydrothermal field associated with felsic domes in the caldera, which NOAA investigators discovered in 2004. A new type of hydrothermal deposit was discovered in the hydrothermal field, consisting of large sulfide-sulfate mounds topped by bulbous constructions of low-temperature Fe and Mn oxides. Vents on the mounds were observed to emit shimmering water. The SZBMVC consists of six closely spaced edifices whose loci are aligned along two parallel trends, one along the volcanic front (Zealandia Bank, Sarigan and South Sarigan), and one about 15 km west towards the rear-arc (Northwest Zealandia, West Zealandia and West Sarigan). Zealandia Bank dives revealed that, as with East Diamante, initial activity was basaltic and became more evolved with time. The western half of Zealandia Bank is dominated by felsic lavas centered on a small (~2 km diameter) caldera and

  1. Temporal evolution of fO2 in the Mariana mantle wedge

    NASA Astrophysics Data System (ADS)

    Brounce, M. N.; Kelley, K. A.; Cottrell, E.

    2013-12-01

    The elevated oxygen fugacity (fO2) recorded by arc lavas appears to be linked to slab fluid influence globally and locally; however, many details regarding the capacity that slab fluids have to oxidize the mantle wedge remain unknown. At the time of subduction initiation, melts may be produced by a combination of decompression and fluid-fluxed melting and the role of flux melting may increase as the subduction zone matures (Reagan et al., 2010, G3 11(3)). Immediately prior to subduction initiation, the mantle presumably has fO2 and fluid concentrations similar to mid-ocean ridge (MOR) source mantle (near the quartz-fayalite-magnetite buffer, QFM, and relatively dry). As subduction zones mature, slab fluids may become more dominant in melt generation as evidenced by increases in fluid-mobile trace element signatures, but the fO2 of slab fluids and temporal changes in the fO2 of erupted basalt remains undocumented. The Mariana forearc southwest of Guam records the initiation of Pacific plate subduction (Reagan et al., 2010). At the base, fore-arc basalts (FAB) erupted at the immediate onset of subduction (51-52 Ma) show minor traces of slab fluid influence and likely reflect decompression melting that occurred as mantle rose to accommodate the sinking Pacific plate. The FAB are overlain by 37-44 Ma 'transitional' basalts whose fluid mobile trace element enrichments indicate that slab fluids have greater influence in their petrogenesis. These lavas provide the opportunity to directly constrain the timescales of mantle wedge oxidation during the initiation and maturation of an oceanic subduction zone. We present Fe3+/ΣFe ratios (μ-XANES) determined on FAB and transitional basalt glasses, paired with previously published major and trace element data. These glasses range in MgO from 2.75 - 7.56 wt% and have Fe3+/ΣFe ratios (0.171 - 0.208) that are slightly more oxidized than MORB (0.16), similar to Mariana trough lavas that reflect minor traces of slab fluid influence

  2. Patgon-Masala Seamount, Southern Mariana Arc: Recent Observations with the WHOI Nereus HROV

    NASA Astrophysics Data System (ADS)

    Fryer, P.; Shank, T. M.; Hulme, S.; Bors, E.

    2009-12-01

    The Patgon-Masala submarine volcano in the southern-most part of the active Mariana arc has the largest caldera in the Mariana system. The seamount rises to 2300 m above the surrounding 3500-m-deep seafloor. The seamount is 20 km long by 12 km wide, elongate NE-SW, paralleling the southern backarc spreading center. The caldera is 5000 m long by 3000 m wide and ~1200 m deep. It has an outer ring soma in the northeast and shows a series of nested inner steps deepening to a maximum of 3159 m in the southwest pit. A Jason-2 lowering in 2003 in this pit permitted us to sample the entire inner north wall of the caldera and recover variably hydrothermally altered lavas of a dominantly basaltic andesite to andesite composition. The lavas are of arc composition, similar to those recovered from the nearby backarc spreading center. This supports the suggestions that the spreading axis and adjacent arc volcanoes share a common magma source (Martinez, et al., 2000; Fryer et al., 2002; Becker, 2005). Below the sill depth of 2650 m, light transmission is less than 75%. Hydrothermal activity was observed in the crater at the contact between the wall and various ledges. In 2000 the Kaiko ROV had performed a dive on the northeast inner wall of the caldera and discovered an active white smoker site, named the Nakayama hydrothermal field (Gamo et al., 2004). In June of 2009 we dove with the Nereus HROV at the same locality during the field-test cruise of the Nereus vehicle. This was the first dive with this vehicle in the vicinity of an active hydrothermal vent. Tether management issues with Nereus on approach to the vent site necessitated a lively collaboration between the scientists, pilots, navigators, and depressor/tether operators. The dive successfully located the Nakayama field and we observed numerous fissures with shimmering water, active hydrothermal venting from a largely pyrite chimney structure, surrounded by a forest of tubeworms, atop an apparent dome of vesicular

  3. Observations of Flatfish "Spas" From Three Hydrothermally Active Seamounts in the Mariana Arc

    NASA Astrophysics Data System (ADS)

    Dower, J.; Tunnicliffe, V.; Tyler, J.; Juniper, K.; Stevens, C.; Kouris, A.; Takano, B.

    2006-12-01

    During a cruise to the Mariana Islands in spring 2004, dense aggregations of small flatfish were recorded from areas of diffuse flow on two hydrothermally active seamounts known as Kasuga-2 and Daikoku. This is quite novel, as flatfish are not known to be part of vent faunas elsewhere. Based on a single specimen, it was determined to be a new species of tonguefish in the genus Symphurus, and is currently under description. In October 2005, we returned to the Mariana Arc and collected about 60 specimens from Kasuga-2, Daikoku, and a third site, Nikko Seamount. Interestingly, the Nikko specimens were about twice as large as the flatfish from Kasuga-2 and Daikoku. Current molecular work (using the Barcode of Life Data System) will determine the relationship among these populations, and verify whether they are the same species. Under the microscope, the sandy sediments from the flatfish habitat were found to be full of tiny nematodes and polychaete worms. Our current hypothesis is that the fish are feeding on both and, thus, are ultimately supported by chemosynthesis, since the worms likely feed on bacteria in the sediments. However, during our most recent cruise in May 2006, we also observed several instances in which dead (or nearly dead) mid-water fish and shrimp fell out of the water column onto the bottom, after which they were almost immediately fed upon by the flatfish. This suggests that there may also be an additional energy subsidy to the seamount benthos from the water column. We hypothesize that sulfite (or some other toxic chemical) in the plume overlying these active volcanoes either kills or anesthetizes small pelagics that get advected over the seamount summit while feeding in near-surface waters at night. Stable isotope and lipid analysis of samples from these "fish spas" are currently underway to establish trophic relationships. We hope to use otolith microstructure analyses to quantify individual growth trajectories and population age structure of

  4. Creation and Deformation of Hydrous Lithosphere at the Southern Mariana Margin

    NASA Astrophysics Data System (ADS)

    Martinez, F.; Kelley, K. A.; Stern, R. J.

    2012-04-01

    Mantle lithosphere formed at mid-ocean seafloor spreading centers is thought to be essentially anhydrous because water is strongly partitioned into melt and removed from the mantle during crustal formation. Since water weakens olivine this dehydration process is also thought to strengthen oceanic mantle lithosphere above solidus depths, perhaps helping to focus deformation and melt delivery to the narrow plate boundary zones observed at mid-ocean ridges. In contrast, convergent margins are sites of high water flux from subducting slabs and thereby provide an opportunity to study the creation and deformation of lithosphere in a hydrous environment. The southern Mariana margin presents a rare case in which the upper plate is undergoing active extension parallel to the trench and directly above the subducting slab. The extension has rifted preexisting Paleogene lithosphere resulting in the present-day creation of new lithosphere in this hydrous environment. Here we present preliminary results from R/V Thomas G. Thompson cruise TN273 in December 2011-January 2012 utilizing the Hawaii Mapping Research Group's IMI-30, a 30 kHz deep-towed side-scan sonar, and ship-based Simrad EM302 multibeam bathymetry. The sidescan sonar imagery and multibeam bathymetry map the tectonic and volcanic structure of a 32 x 80 km area referred to as the southeast Mariana forearc rifts (SEMFR), which extend from near the backarc spreading center toward the trench. The sonar imagery shows a complex volcanic and tectonic structure with no single spreading or rifting axis. Volcanism appears to be widely dispersed and separated by faulted areas. Bathymetry data show several rifts spanning this area but no single rift appears to be focusing tectonic activity as earthquake seismicity is broadly distributed across this region. The data depict a broad volcano-tectonic zone of complex deformation and distributed volcanism unlike the narrow plate boundary zones of mid-ocean ridges. This distributed

  5. Lithium Isotope Study of Peridotite-Slab Fluid Interactions in the Mariana Forearc Mantle Wedge

    NASA Astrophysics Data System (ADS)

    Lui-Heung, C.; Savov, I. P.; Ryan, J. G.

    2006-05-01

    Drilling of the Mariana forearc region during ODP Leg 195 at S. Chamorro Seamount recovered serpentinized peridotite clasts enclosed by serpentinite muds and slab-derived porefluids. In the serpentinized peridotite clasts the ranges of Li abundance (1- 8 ppm) and δ7Li (-1.4 to +5 ‰) are similar to those reported for the forearc- serpentinites of the Conical Seamount [Benton et al., 2004]. Although the serpentinized muds show Li abundances comparable to the clasts (0.5- 10 ppm; average: 2.5 ppm), they have higher δ7Li (+13.4 ‰). Heavier isotopic composition of serpentinized muds confirms the inferences from REE modeling and the visual observations for the presence of 5-10 % metabasalt component, most probably from the Pacific oceanic crust. Fluids (pH up to 12.5) circulating within the muds are highly depleted in Li and show dramatic 7Li-enrichment relative to seawater (δ7Li up to 47 per mil). These characteristics are consistent with near complete removal of Li from slab fluids into minerals of the sepentinite assemblage. We will present a model to describe the fluid-rock interaction including a reconstruction of the Li isotope compositions of the fluids as they leave the subducted slab assemblage, before transiting through the Mariana mantle wedge. Our model will evaluate the importance of Raleigh- type distillation processes under high P/low T conditions within the sub-arc mantle. Because both Conical and S.Chamorro seamounts are sampling approx. 30 km deep column of similarly depleted mantle wedge immediately above the subducting Pacific plate, the clasts may have similar Li systematics due to the effect of equally long transit through the mantle. The similarity in δ7Li signature among all arc lavas implies that although the ultimate source of Li is the subducting slab, the final Li isotope makeup of arc magmatic suites is controlled by the degree of mantle wedge- fluid/ (melt?) interaction upon emplacement.

  6. Subduction-related B and H Isotope Fractionations Across the Mariana arc - Consequences for Recycling

    NASA Astrophysics Data System (ADS)

    Shaw, A. M.; Hauri, E. H.; Stern, R.; Hawkins, J.; Gurenko, A.

    2009-05-01

    We present new B and H isotope data, along with volatile, trace and major elements for olivine-hosted melt inclusions from a suite of cross chain volcanoes extending across the Mariana arc from Guguan volcano to the Mariana Trough. H and B isotopes, H2O, CO2, S, F and Cl abundances, as well as trace elements, have been determined by SIMS. Our results show that enrichments in fluid-mobile elements generally associated with the subducting slab (e.g., Ba, B) decrease systematically across the arc into the back-arc. However, water contents in cross-chain samples, 230 km above the subducting slab, show similar values to the arc-front samples, implying that water release is a continuous process across the arc and that trace element proxies for slab fluids are decoupled from actual water contents. The isotopic composition of water changes during progressive dehydration, as expected1; δD values are highest at the arc front, ˜ -10‰, and decrease to values as low as -80‰ in the back-arc. Likewise, B isotopes decrease systematically from δ11B values as high as 5.9-8.5‰ at the arc front, consistent with values previously reported for Guguan arc front lavas (δ11B = 5.0-6.2‰2), down to values as low as -14‰ in back-arc melt inclusions, extending beyond the range inferred for MORB (-5 to -9‰3) and to values observed in OIBs (down to -15‰4). Our observations demonstrate that fluid release behind the main volcanic front can be substantial and that the dehydration process has fractionated H and B isotope compositions. Our findings suggest that B and H isotopes of OIBs containing recycled slab components would be low. 1 Shaw et al. (2008) EPSL v.275, 138-145 2 Ishikawa and Tera (1999) Geology v.27, 83-86 3 leRoux et al. (2003) Abstr. Fall AGU #V51A-03 4 Chaussidon and Marty (1995) Science v.269, 383-386

  7. Emplacement, growth, and gravitational deformation of serpentinite seamounts on the Mariana forearc

    NASA Astrophysics Data System (ADS)

    Oakley, A. J.; Taylor, B.; Fryer, P.; Moore, G. F.; Goodliffe, A. M.; Morgan, J. K.

    2007-08-01

    Serpentinite seamounts, representing some of the first material outputs of the recycling process that takes place in subduction zones, are found on the outer Mariana forearc. Multichannel seismic (MCS) and bathymetric data collected in 2002 image the large-scale structures of five seamounts, as well as the pre-seamount basement geometry and sediment stratigraphy. We present data from three edifices that provide insights into seamount growth and internal deformation processes and allow us to support the interpretation that serpentinite mud volcanoes are formed by the episodic eruption of mud flows from a central region. The presence of thrust faulting at the base of Turquoise and Big Blue Seamounts, along with the low surface slopes (5°-18°) of all the seamounts studied, lead us to infer that these edifices spread laterally and are subject to gravitational deformation as they grow. Numerical simulations using the discrete element method (DEM) were used to model their growth and the origins of features that we see in MCS sections, such as basal thrusts, inward-dipping reflections and mid-flank benches. The DEM simulations successfully reproduced many of the observed features. Simulations employing very low basal and internal friction coefficients (~0.1 and ~0.4, respectively) provide the best match to the overall morphology and structures of the serpentinite seamounts. However the simulations do not capture all of the processes involved in seamount growth, such as withdrawal of material from a central conduit leading to summit deflation; compaction, dewatering and degassing of mud flows; mass wasting in the form of sector collapse and growth upon a dipping substrate. A strong reflection beneath the summit of Big Blue, the largest serpentinite seamount on the Mariana forearc, represents the floor of a summit depression that has been partially in-filled by younger muds, supporting the idea that serpentinite seamounts grow by episodic mud volcanism. Boundaries of mud

  8. Discovery of Active Hydrothermal Sites Along the Mariana Volcanic Arc, Western Pacific Ocean

    NASA Astrophysics Data System (ADS)

    Baker, E. T.; Embley, R. W.; Resing, J. A.; Lupton, J. E.; Massoth, G. J.; de Ronde, C. E.; Nakamura, K.; Walker, S. L.

    2003-12-01

    Some 20,000 km of volcanic arcs, roughly one-third the total length of the global midocean ridge (MOR) system, rim the western Pacific Ocean. But compared to 25 years of hydrothermal investigations along MORs, exploration of similar activity on the estimated 600 submarine arc volcanoes is only beginning. In February 2003, as part of the Submarine Ring of Fire project funded by NOAA's Ocean Exploration Program, we made the first systematic survey of hydrothermal activity along the 1270-km-long Mariana intraoceanic volcanic arc, which lies almost entirely within the US EEZ. Prior fieldwork had documented active (but low-temperature) hydrothermal discharge on only three volcanoes: Kasuga 2, Kasuga 3, and Esmeralda Bank. During the cruise, we conducted 70 CTD operations over more than 50 individual volcanoes from 13° N to 23° N, plus a continuous CTD survey along 75 km of the back-arc spreading center (13° 15'N to 13° 41'N) adjacent to the southern end of the arc. We found evidence for active hydrothermal venting at 11 submarine volcanoes with summit (or caldera floor) depths ranging from 50 to 1550 m. Two additional sites were identified on the back-arc spreading center. Ongoing analyses of collected water samples could increase these totals. Our results confirmed continuing hydrothermal activity at Kasuga 2 (but not Kasuga 3) and Esmeralda Bank, in addition to newly discovered sites on nine other volcanoes. Many of these sites produce intense and widely dispersed plumes indicative of vigorous, high-temperature discharge. The volcanoes with active hydrothermal systems are about equally divided between those with and without summit calderas. The addition of the Marianas data greatly improves our view of hydrothermal sources along arcs. The 20,000 km of Pacific arcs can be divided between 6380 km of intraoceanic (i.e., mostly submarine) arcs and 13,880 km of island (i.e., mostly subaerial) arcs. At present, ˜15% of the total length of Pacific arcs has been surveyed

  9. A novel large filamentous deltaproteobacterium on hydrothermally inactive sulfide chimneys of the Southern Mariana Trough

    NASA Astrophysics Data System (ADS)

    Kato, Shingo; Yamagishi, Akihiko

    2016-04-01

    Unusual large filamentous bacteria (LFB) have been found on the deep seafloor environments. They play a significant role in geochemical cycling in the dark environments. However, our knowledge of the spatial distribution and phylogenetic diversity of the LFB on the deep seafloor are still limited due to the inaccessibility to these environments. Here, we report the discovery of a novel LFB on a hydrothermally inactive sulfide chimney in a deep-sea hydrothermal field of the Southern Mariana Trough. Light and electron microscopic observation showed that the width and total length of the LFB were >8 μm and >100 μm, respectively, of which morphology was similar to that of other known LFB such as "cable bacteria" of the Desulfobulbaceae. Analyses of a 16S rRNA gene clone library and fluorescence in situ hybridization revealed that this LFB belongs to the Desulfobulbaceae. The 16S rRNA gene of the LFB showed 94% similarity to those of the reported cable bacteria and cultured deltaproteobacterial species, suggesting that the LFB is a novel cable bacterium of the Desulfobulbaceae. The novel LFB potentially play a role in sulfur cycling on sulfide chimneys at the hydrothermally ceasing or even ceased deep-sea hydrothermal fields.

  10. Sub-seafloor bacterial community structures within massive sulfide deposits at the Southern Mariana Trough

    NASA Astrophysics Data System (ADS)

    Yamagishi, A.; Kato, S.; Moriya, O.; Urabe, T.

    2012-12-01

    Yet-uncharacterized sub-seafloor bacterial communities within massive sulfide deposits important for ocean elemental flux were investigated by 16S rRNA gene analysis. Shallow sub-seafloor drilling was performed in a deep-sea hydrothermal field (called Pika site) of the Southern Mariana Trough (SMT). The borehole length was 4.252 m and the length of the core sample obtained was 2,920 mm (¬68.7% recovery). The core sample mainly consisted of pyrite (FeS2), sphalerite (ZnS) and chalcopyrite (CuFeS2), and contained barite (BaSO4) as minor components. Three subsamples (upper, middle and lower) of the core sample were picked up from different depth points (0.77, 1.14, 2.37 mbsf). Bacterial 16S rRNA gene analysis was performed for the three subsamples. The community structures based on the detected clones were clearly different from one another. The dominant clone detected in each subsample was related to Gammaproteobacteria, Bacteroidetes and Ignavibacteria, or Nitrospirae, respectively. Many clones detected from the sub-seafloor massive sulfide deposits were similar to those from sulfide chimneys of inactive vents or basaltic lavas on the seafloor including SMT and other areas. Comparative analysis revealed the commonality and difference of the community structures with those in surrounding seafloor environments depending on the sampling depth.

  11. Near-bottom magnetic surveys around hydrothermal sites in the southern Mariana Trough

    NASA Astrophysics Data System (ADS)

    Nogi, Y.; Mochizuki, N.; Okino, K.; Asada, M.

    2011-12-01

    Near-bottom magnetic survey is an effective method to reveal detailed magnetic anomaly features of seafloor. The measurements of three-components of the geomagnetic field by using AUV "URASHIMA" were conducted during the YK-09-08 cruise in the southern Mariana Trough in order to detect signals of hydrothermally altered rocks. During the cruise, vector geomagnetic field are successfully obtained along the all dive tracks with the information of the vehicle's attitude. Total intensities of geomagnetic field by the overhauser magnetometer were also conducted, but the data are only collected along almost E-W oriented observation lines due to the sensitivity of the sensor. The distribution of crustal magnetization are estimated using downward component of magnetic anomalies by the inversion method. The distribution of low crustal magnetization are almost coincide with the area around hydrothermal vent sites from on ridge to off ridge area, and most likely indicate signs of hydrothermally altered rocks. The distribution of low crustal magnetization on ridge are almost parallel to the the strike of ridge axis implying tectonic control of hydrothermal vent sites.

  12. Review of Mariana Gosnell's 'ICE: The Nature, the History, and the Uses of an Astonishing Substance'

    NASA Technical Reports Server (NTRS)

    Parkinson, Claire L.

    2006-01-01

    ICE: The Nature, the History, and the Uses of an Astonishing Substance is a recently published book by Mariana Gosnell about ice. It covers not just the ice that is readily seen, such as sea ice, lake ice, icebergs, glaciers, ice sheets, and ice cubes, but also ice in the ground, in the atmosphere, inside plants and animals, and in outer space, plus new ice forms being created in scientific laboratories. Gosnell treats the reader to a well-written, easy-going mixture of science, adventure, history, applications, science methods and controversies, and philosophy, all centered in one way or another on ice. The book is 563 pages long and is filled with fascinating anecdotes and details, such as beetles in the Canadian Rockies that can supercool to 60 C below freezing and a lake in Minnesota where each winter typically 65,000 fishing shanties are set up on the lake's ice, many with couches, beds, television sets, and bathrooms. Gosnell also includes many practical suggestions. Among them: When driving on lake ice, keep your windows open, in case your vehicle breaks through the ice and you need to make a rapid exit.

  13. Mapping the fluid flow of the Mariana Mounds ridge flank hydrothermal system: Pore water chemical tracers

    SciTech Connect

    Wheat, C.G.; McDuff, R.E.

    1995-05-10

    The authors present a conceptual model of fluid circulation in a ridge flank hydrothermal system, the Mariana Mounds. The model is based on chemical data from pore waters extracted from piston cores and from push cores collected by deep-sea research vessel Alvin in small, meter-sized mounds situated on a local topographic high. These mounds are located within a region of heat flow exceeding that calculated from a conductive model and are zones of strong pore water upflow. The authors have interpreted the chemical data with time-dependent transport-reaction models to estimate pore water velocities. In the mounds themselves pore water velocities reach several meters per year to kilometers per year. Within about 100 m from these zones of focused upflow velocities decrease to several centimeters per year up to tens of centimeters per year. A large area of low heat flow surrounds these heat flow and topographic highs, with upwelling pore water velocities less than 2 cm/yr. In some nearby cores, downwelling of bottom seawater is evident but at speeds less than 2 cm/yr. Downwelling through the sediments appears to be a minor source of seawater recharge to the basaltic basement. The authors conclude that the principal source of seawater recharge to basement is where basement outcrops exist, most likely a scarpt about 2-4 km to the east and southeast of the study area. 71 refs., 14 figs., 3 tabs.

  14. Environmental Factors Affecting Large-Bodied Coral Reef Fish Assemblages in the Mariana Archipelago

    PubMed Central

    Richards, Benjamin L.; Williams, Ivor D.; Vetter, Oliver J.; Williams, Gareth J.

    2012-01-01

    Large-bodied reef fishes represent an economically and ecologically important segment of the coral reef fish assemblage. Many of these individuals supply the bulk of the reproductive output for their population and have a disproportionate effect on their environment (e.g. as apex predators or bioeroding herbivores). Large-bodied reef fishes also tend to be at greatest risk of overfishing, and their loss can result in a myriad of either cascading (direct) or indirect trophic and other effects. While many studies have investigated habitat characteristics affecting populations of small-bodied reef fishes, few have explored the relationship between large-bodied species and their environment. Here, we describe the distribution of the large-bodied reef fishes in the Mariana Archipelago with an emphasis on the environmental factors associated with their distribution. Of the factors considered in this study, a negative association with human population density showed the highest relative influence on the distribution of large-bodied reef fishes; however, depth, water temperature, and distance to deep water also were important. These findings provide new information on the ecology of large-bodied reef fishes can inform discussions concerning essential fish habitat and ecosystem-based management for these species and highlight important knowledge gaps worthy of additional research. PMID:22384014

  15. Crustal and Uppermost Mantle Shear Velocity Structure across the Mariana Trench

    NASA Astrophysics Data System (ADS)

    Cai, C.; Wiens, D.; Lizarralde, D.

    2015-12-01

    We investigate the shear wave structure of the crust and uppermost mantle across the Northern and Central Mariana trench using data recorded by a temporary network deployed in 2012-2013, including 7 island stations and 20 ocean bottom seismographs (OBSs). The goal of this study is to constrain velocity variations resulting from possible serpentinization of the incoming plate and the forearc mantle, and thus better understand the water budget of subduction zones. We use an ambient noise analysis method to reveal surface wave phase velocities at short period (8-30 s). Vertical component cross correlations show distinct fundamental and 1st higher-mode Rayleigh wave signals, especially between station pairs in the incoming plate. The 1st higher-mode Rayleigh wave is of significant importance for resolving crustal and shallowest upper mantle structure in regions covered by deep water. A multichannel cross correlation method (Eikonal tomography) is applied to Rayleigh waves from teleseismic earthquakes to obtain phase velocity at 25-80 s period. Finally the combined phase velocity curve from noise and teleseismic earthquakes is inverted at each point to determine the shear velocity structure. Preliminary inversion results for the incoming plate indicate slightly thickened crust that may be caused by a large Cretaceous igneous event associated with nearby seamounts. A high velocity anomaly emerges at around 20 km depth in the same region. We will present the entire 3-D shear wave velocity model across the trench during the AGU fall meeting.

  16. Geological aspects of the 2003 2004 eruption of Anatahan Volcano, Northern Mariana Islands

    NASA Astrophysics Data System (ADS)

    Nakada, Setsuya; Matsushima, Takeshi; Yoshimoto, Mitsuhiro; Sugimoto, Takeshi; Kato, Teruyuki; Watanabe, Tsuyoshi; Chong, Ramon; Camacho, Juan T.

    2005-08-01

    Anatahan Volcano, Northern Mariana Islands, began erupting in May-June 2003. A series of subplinian explosive eruptions of andesite magma began at the Eastern Crater in the eastern part of the summit caldera on the evening of 10 May. Brown tephra was sent mainly westward by strong winds. Small-scale pyroclastic surges were discharged eastward outside the caldera in late May. An andesite lava dome that had once filled the inner crater was fragmented by phreatomagmatic explosions in the middle of June. The phreatomagmatic explosions probably occurred due to interaction of the magma head with groundwater around the crater, and abundant very fine ash ("gray tephra") was discharged within the caldera and over most of the island. The volume of eruption products of the May-June eruption was estimated to be 1.4 × 10 7 m 3 dense-rock-equivalent. Erupted pumices and lava are aphyric andesite and are variously colored depending on their vesicularity. The SiO 2 contents of erupted materials decreased slightly with time. The fine gray ash is depleted in alkalies, probably due to leaching by acid hydrothermal fluids during explosions. Seismic activity resumed in late March 2004, and small strombolian-like explosions were repeated in May and June 2004. About half of the inner crater was filled with new scoria and lava.

  17. Ground-water resources of Tinian, Commonwealth of the Northern Mariana Islands

    USGS Publications Warehouse

    Gingerich, Stephen B.; Yeatts, Daniel S.

    2000-01-01

    Tinian, which lies in the western Pacific Ocean at latitude 15°N and longitude 145°W (fig. 1), is the second largest island (39.2 mi2) in the Commonwealth of the Northern Mariana Islands (CNMI). Fresh ground water is obtained from shallow wells that tap the surface of a freshwater lends found in an aquifer composed mainly of coralline limestone. The main water-supply well withdraws water with a chloride concentration ranging from 160 to 220 mg/L. Current (1999) pumping rates adequately supply the island residents but future demand are expected to be higher.. To better understand the ground-water resources of the island and to learn more about the hydrology of oceanic islands, the U.S. Geological Survey (USGS) entered into a cooperative study with the Municipality of Tinian. The objective of the study, conducted between 1990 and 1997, was to assess the ground-water resources of the is;land. This report presents some of the results of the study including a description of the island's geology and geography, the current land use, the water-production system, the thickness and arcal extent of the freshwater lens, the water-table configuration and directions of ground-water flow. The report also discusses the relation of the changes in water-table elevation to daily and seasonal changes in ocean level.

  18. Status and Natural History of Emballonura Semicaudata Rotensis on Aguiguan, Mariana Islands

    USGS Publications Warehouse

    Wiles, Gary J.; O'Shea, Thomas J.; Worthington, David J.; Esselstyn, Jacob A.; Valdez, Ernest W.

    2011-01-01

    Pacific sheath-tailed bats (Emballonura semicaudata rotensis) in the Mariana Islands declined greatly in abundance and distribution during the 20th century. The small island of Aguiguan now supports the only persisting population. We studied abundance and natural history of this population from 1995–2008. There was a likely population increase during the study, with 359–466 (minimum and maximum) bats counted at caves in 2008. Bats roosted only in caves, primarily those of relatively larger size. Bats were detected in only seven of 95 caves; three caves were always occupied when surveyed. One cave consistently had the largest colony ( ± SD = 333 ± 33.6 in 2008). Others held 1–64 bats. Cave environments showed no complexities in temperature or humidity. Preliminary observations indicate a litter size of one and the possibility of birthing timed to coincide with the transitional period leading into the rainy season (June–July). We review potential threats to E. s. rotensis on Aguiguan and make suggestions for conservation.

  19. Green synthesis of β-sitostanol esters catalyzed by the versatile lipase/sterol esterase from Ophiostoma piceae.

    PubMed

    Molina-Gutiérrez, María; Hakalin, Neumara L S; Rodríguez-Sanchez, Leonor; Prieto, Alicia; Martínez, María Jesús

    2017-04-15

    β-sitostanol esters, used as dietary complement for decreasing cholesterol absorption, have been synthesized at 28°C via direct esterification or transesterification catalyzed by the versatile lipase/sterol esterase from the ascomycete fungus O. piceae. Direct esterification was conducted in biphasic isooctane: water systems containing 10mM β-sitostanol and lauric or oleic acid as acyl donors, reaching 90% esterification in 3h with the recombinant enzyme. The use of molar excesses of the free fatty acids did not improve direct esterification rate, and the enzyme did not convert one of the two fatty acids preferentially when both were simultaneously available. On the other hand, solvent-free transesterification was an extremely efficient mechanism to synthesize β-sitostanyl oleate, yielding virtually full conversion of up to 80mM β-sitostanol in 2h. This process may represent a promising green alternative to the current chemical synthesis of these esters of unquestionable nutraceutical value.

  20. Effect of bark beetle (Ips typographus L.) attack on bark VOC emissions of Norway spruce (Picea abies Karst.) trees

    NASA Astrophysics Data System (ADS)

    Ghimire, Rajendra P.; Kivimäenpää, Minna; Blomqvist, Minna; Holopainen, Toini; Lyytikäinen-Saarenmaa, Päivi; Holopainen, Jarmo K.

    2016-02-01

    Climate warming driven storms are evident causes for an outbreak of the European spruce bark beetle (Ips typographus L.) resulting in the serious destruction of mature Norway spruce (Picea abies Karst.) forests in northern Europe. Conifer species are major sources of biogenic volatile organic compounds (BVOCs) in the boreal zone. Climate relevant BVOC emissions are expected to increase when conifer trees defend against bark beetle attack by monoterpene (MT)-rich resin flow. In this study, BVOC emission rates from the bark surface of beetle-attacked and non-attacked spruce trees were measured from two outbreak areas, Iitti and Lahti in southern Finland, and from one control site at Kuopio in central Finland. Beetle attack increased emissions of total MTs 20-fold at Iitti compared to Kuopio, but decreased the emissions of several sesquiterpenes (SQTs) at Iitti. At the Lahti site, the emission rate of α-pinene was positively correlated with mean trap catch of bark beetles. The responsive individual MTs were tricyclene, α-pinene, camphene, myrcene, limonene, 1,8-cineole and bornyl acetate in both of the outbreak areas. Our results suggest that bark beetle outbreaks affect local BVOC emissions from conifer forests dominated by Norway spruce. Therefore, the impacts of insect outbreaks are worth of consideration to global BVOC emission models.

  1. Demonstration of paternal inheritance of plastids in Picea (Pinaceae). [Hybridization of cloned, sup 32 -P labeled, petunia cpDNA

    SciTech Connect

    Stine, M.

    1988-01-01

    Chloroplast DNA (cpDNA) was purified from Picea glauca, P. pungens, P. engelmannii, and P. omorika, and was digested with several restriction endonucleases. Interspecific restriction fragment length polymorphisms (RFLPs) of cpDNA were identified. The RFLPs were identified as cpDNA by the hybridization of cloned, {sup 32}-P labeled, petunia cpDNA to the polymorphic bands, and by the lack of hybridization of a cloned and labeled mtDNA probe from maize. Chloroplast DNA RFLPs that showed no intraspecific variation when examined across the natural range for each species, were used as markers to follow the inheritance of plastids in interspecific hybrids. The inheritance of plastids was determined for F{sub 1}-hybrids from reciprocal crosses of P. glauca and P. pungens, P. glauca and P. omorika, and F{sub 1}-hybrids of P. engelmannii x pungens. All 31 F{sub 1}-hybrids examined showed the cpDNA genotypes of the pollen parent, or the paternal species.

  2. Engelmann Spruce (Picea engelmannii) as a biological monitor of changes in soil metal loading related to past mining activity

    USGS Publications Warehouse

    Witte, K.M.; Wanty, R.B.; Ridley, W.I.

    2004-01-01

    Engelmann spruce (Picea engelmannii) is the dominant tree species in many abandoned mine areas of the Rocky Mountains. It is long-lived, and therefore, may act as a long term biological monitor of changes in soil chemistry caused by past mining activity. In this study, laser ablation inductively coupled mass spectrometry (LA-ICPMS) was used to analyze individual tree rings of Engelmann spruce for Fe, Zn, Cu, Cd, Mn, Pb and Sr concentrations. Cores were obtained from trees growing in tailings-impacted and control (non-tailings impacted) sites near the Waldorf mine (Waldorf, CO, USA). Zinc, Cu, Fe, Cd, Pb and Sr concentrations remained low and consistent over time in the control tree rings. However, in the tailings impacted cores, concentrations of Zn, Cu, Fe and Cd increase significantly in post-mining rings. In addition, Zn, Cu, Fe, and Cd concentrations in pre-mining rings of both the control and tailings impacted cores are similar, indicating that present day soil concentrations of these elements in the control area are a reasonable estimation of background for this area. Lead and Sr concentrations in control and tailings-impacted rings remained similar and relatively constant through time and are not useful in determining changes in soil chemistry due to past mining activity. ?? 2004 Elsevier Ltd. All rights reserved.

  3. [Spatial distribution characteristics of the biomass and carbon storage of Qinghai spruce (Picea crassifolia) forests in Qilian Mountains].

    PubMed

    Peng, Shou-zhang; Zhao, Chuan-yan; Zheng, Xiang-lin; Xu, Zhong-lin; He, Lei

    2011-07-01

    This paper estimated the biomass and carbon storage and their spatial distributions of Qinghai spruce (Picea crassifolia) forests in Qilian Mountains, based on the field investigation, forest map, and meteorological data, and with the help of GIS technology. In 2008, the biomass of the forests was averagely 209.24 t x hm(-2), with a total biomass of 3.4 x 10(7) t. Due to the difference of water and thermal condition, there existed great differences in the biomass of Qinghai spruce within the Mountains. The biomass increased by 3.12 t x hm(-2) with increasing 1 degrees longitude and decreased by 3.8 t x hm(-2) with increasing 1 degrees latitude, and decreased by 0.05 t x hm(-2) with the elevation increasing 100 m. The carbon density of the forests ranged from 70.4 to 131.1 t x hm(-2), averagely 109.8 t x hm(-2), and the average carbon density was 83.8 t x hm(-2) for the young forest, 109.6 t x hm(-2) for the middle age forest, 122 t x hm(-2) for the near-mature forest, 124.2 t x hm(-2) for the mature forest, and 117.1 t x hm(-2) for the over-mature forest. The total carbon storage of Qinghai spruce forests in the study area was 1.8 x 10(7) t.

  4. Differential regulation of Knotted1-like genes during establishment of the shoot apical meristem in Norway spruce (Picea abies).

    PubMed

    Larsson, Emma; Sitbon, Folke; von Arnold, Sara

    2012-06-01

    Establishment of the shoot apical meristem (SAM) in Arabidopsis embryos requires the KNOXI transcription factor SHOOT MERISTEMLESS. In Norway spruce (Picea abies), four KNOXI family members (HBK1, HBK2, HBK3 and HBK4) have been identified, but a corresponding role in SAM development has not been demonstrated. As a first step to differentiate between the functions of the four Norway spruce HBK genes, we have here analyzed their expression profiles during the process of somatic embryo development. This was made both under normal embryo development and under conditions of reduced SAM formation by treatment with the polar auxin transport inhibitor NPA. Concomitantly with the formation of an embryonic SAM, the HBK2 and HBK4 genes displayed a significant up-regulation that was delayed by NPA treatment. In contrast, HBK1 and HBK3 were up-regulated prior to SAM formation, and their temporal expression was not affected by NPA. Ectopic expression of the four HBK genes in transgenic Arabidopsis plants further supported similar functions of HBK2 and HBK4, distinct from those of HBK1 and HBK3. Together, the results suggest that HBK2 and HBK4 exert similar functions related to the SAM differentiation and somatic embryo development in Norway spruce, while HBK1 and HBK3 have more general functions during embryo development.

  5. Improved white spruce (Picea glauca) genome assemblies and annotation of large gene families of conifer terpenoid and phenolic defense metabolism.

    PubMed

    Warren, René L; Keeling, Christopher I; Yuen, Macaire Man Saint; Raymond, Anthony; Taylor, Greg A; Vandervalk, Benjamin P; Mohamadi, Hamid; Paulino, Daniel; Chiu, Readman; Jackman, Shaun D; Robertson, Gordon; Yang, Chen; Boyle, Brian; Hoffmann, Margarete; Weigel, Detlef; Nelson, David R; Ritland, Carol; Isabel, Nathalie; Jaquish, Barry; Yanchuk, Alvin; Bousquet, Jean; Jones, Steven J M; MacKay, John; Birol, Inanc; Bohlmann, Joerg

    2015-07-01

    White spruce (Picea glauca), a gymnosperm tree, has been established as one of the models for conifer genomics. We describe the draft genome assemblies of two white spruce genotypes, PG29 and WS77111, innovative tools for the assembly of very large genomes, and the conifer genomics resources developed in this process. The two white spruce genotypes originate from distant geographic regions of western (PG29) and eastern (WS77111) North America, and represent elite trees in two Canadian tree-breeding programs. We present an update (V3 and V4) for a previously reported PG29 V2 draft genome assembly and introduce a second white spruce genome assembly for genotype WS77111. Assemblies of the PG29 and WS77111 genomes confirm the reconstructed white spruce genome size in the 20 Gbp range, and show broad synteny. Using the PG29 V3 assembly and additional white spruce genomics and transcriptomics resources, we performed MAKER-P annotation and meticulous expert annotation of very large gene families of conifer defense metabolism, the terpene synthases and cytochrome P450s. We also comprehensively annotated the white spruce mevalonate, methylerythritol phosphate and phenylpropanoid pathways. These analyses highlighted the large extent of gene and pseudogene duplications in a conifer genome, in particular for genes of secondary (i.e. specialized) metabolism, and the potential for gain and loss of function for defense and adaptation.

  6. ,* Copper transport and accumulation in spruce stems (picea abies(L.) Karsten) revelaed by laser-induced breakdown spectroscopy

    SciTech Connect

    Krajcarova, Dr. Lucie; Novotny, Dr. Karel; Babula, Dr. Petr; Pravaznik, Dr Ivo; Kucerova, Dr. Petra; Vojtech, Dr. Adam; Martin, Madhavi Z; Kizek, Dr. Rene; Kaiser, Jozef

    2013-01-01

    Laser-Induced Breakdown Spectroscopy (LIBS) in double pulse configuration (DP LIBS) was used for scanning elemental spatial distribution in annual terminal stems of spruce (Picea abies (L.) Karsten). Cross sections of stems cultivated in Cu2+ solution of different concentrations were prepared and analyzed by DP LIBS. Raster scanning with 150 m spatial resolution was set and 2D (2-dimentional) maps of Cu and Ca distribution were created on the basis of the data obtained. Stem parts originating in the vicinity of the implementation of the cross sections were mineralized and subsequently Cu and Ca contents were analyzed by inductively coupled plasma mass spectrometry (ICP-MS). The results provide quantitative information about overall concentration of the elements in places, where LIBS measurements were performed. The fluorescence pictures were created to compare LIBS distribution maps and the fluorescence intensity (or the increase in autofluorescence) was used for the comparison of ICP-MS quantitative results. Results from these three methods can be utilized for quantitative measurements of copper ions transport in different plant compartments in dependence on the concentration of cultivation medium and/or the time of cultivation.

  7. Development of growth media for solid substrate propagation of ectomycorrhizal fungi for inoculation of Norway spruce (Picea abies) seedlings.

    PubMed

    Vuorinen, Irmeli; Hamberg, Leena; Müller, Michael; Seiskari, Pekka; Pennanen, Taina

    2015-05-01

    A silica-based propagation medium was developed for large-scale production of ectomycorrhizal (ECM) fungal inoculum by solid state fermentation. Development of the medium was started by screening for an optimal growth medium among six different semisynthetic agar media traditionally used in cultivation of ECM fungi. The majority (65 %) of the twenty tested ECM fungal strains that typically colonize Norway spruce (Picea abies) seedlings grew best on modified Melin-Norkrans (MMN) medium with reduced sugar content (½MMN). In order to develop a nutritionally similar medium for large-scale cultivation of the ECM fungi, we chose silica to form a solid matrix and light brewery malt extract to provide nutrients. The medium was supplemented with a commercial humic acid product that was shown to boost fungal growth. The optimal concentration of the constituents was screened for in two assays by determining the growth rates of seven potential inoculant ECM fungal strains (Amphinema sp., Cenococcum geophilum, Hebeloma sp., Meliniomyces bicolor, Paxillus involutus, Piloderma byssinum, and Tylospora asterophora). As a result, we composed a silica-based mass propagation medium (pH 5.8) containing 2.5 % brewery malt extract and 0.5 g/l humic acid product Lignohumate AM. This medium is easily produced and supported good growth of even the slowly growing and rarely studied Athelioid ECM strains. Furthermore, root systems of Norway spruce nursery seedlings were colonized by the tested ECM fungi by using solid inoculum formulated from the silica medium.

  8. Effect of debarking water from Norway spruce (Picea abies) on the growth of five species of wood-decaying fungi.

    PubMed

    Edfeldt, Amelie Fagerlund; Hedenström, Erik; Edman, Mattias; Jonsson, Bengt Gunnar

    2014-01-01

    Norway spruce (Picea abies) debarking water is an aqueous extract obtained as waste from the debarking of logs at paper mills. The debarking water contains a mixture of natural compounds that can exhibit diverse biological activities, potentially including fungicidal activity on some species of wood-decaying fungi. Thus, we investigated the growth rates of such fungi on agar plates to which debarking water extracts had been added. The experiment included five wood-decaying fungi, viz. Gloeophyllum sepiarium, Oligoporus lateritius, Ischnoderma benzoinum, Junghuhnia luteoalba, and Phlebia sp. Growth reduction was observed for all species at the highest tested concentrations of freeze-dried and ethanol-extracted debarking water, the ethyl acetate-soluble fraction and the diethyl ether-soluble fraction. However, the magnitude of the effect varied between different species and strains of individual species. The brown-rot fungi G. sepiarium and O. lateritius were generally the most sensitive species, with the growth of all tested strains being completely inhibited by the ethyl acetate-soluble fraction. These results indicate that development of antifungal wood-protecting agents from debarking water could potentially be a way to make use of a low-value industrial waste.

  9. Effects of warming on ectomycorrhizal colonization and nitrogen nutrition of Picea asperata seedlings grown in two contrasting forest ecosystems

    PubMed Central

    Li, Yuejiao; Sun, Didi; Li, Dandan; Xu, Zhenfeng; Zhao, Chunzhang; Lin, Honghui; Liu, Qing

    2015-01-01

    Ectomycorrhiza (ECM) plays an important role in plant nitrogen (N) nutrition and regulates plant responded to climate warming. We conducted a field experiment in a natural forest and a plantation in the eastern Tibetan Plateau to estimate the warming effects of open-top chambers (OTC) on ECM and N nutrition of Picea asperata seedlings. Four-year warming significantly decreased ECM colonization, ECM fungal biomass, fine root vigor, and the N concentration of leaf, stem and coarse root, but significantly increased fine root N concentration and N content of leaf, stem, fine root and whole plant in natural forest. Contrarily, warming induced no obvious change in most of these parameters in plantation. Moreover, warming decreased rhizospheric soil inorganic N content in both forests. Our results showed that four-year warming was not beneficial for ECM colonization of P. asperata seedlings in the two forests, and the seedlings in natural forest were more sensitive and flexible to experimental warming than in plantation. The changes of ECM colonization and fine root biomass for effective N uptake would be good for plant growth and remit N leaching under future warming in natural forest. PMID:26655633

  10. Forest microsite effects on community composition of ectomycorrhizal fungi on seedlings of Picea abies and Betula pendula.

    PubMed

    Tedersoo, Leho; Suvi, Triin; Jairus, Teele; Kõljalg, Urmas

    2008-05-01

    Niche differentiation in soil horizons, host species and natural nutrient gradients contribute to the high diversity of ectomycorrhizal fungi in boreal forests. This study aims at documenting the diversity and community composition of ectomycorrhizal fungi of Norway spruce (Picea abies) and silver birch (Betula pendula) seedlings in five most abundant microsites in three Estonian old-growth forests. Undisturbed forest floor, windthrow mounds and pits harboured more species than brown- and white-rotted wood. Several species of ectomycorrhizal fungi were differentially represented on either hosts, microsites and sites. Generally, the most frequent species in dead wood were also common in forest floor soil. Ordination analyses suggested that decay type determined the composition of EcM fungal community in dead wood. Root connections with in-growing mature tree roots from below affected the occurrence of certain fungal species on seedling roots systems in dead wood. This study demonstrates that ectomycorrhizal fungi differentially establish in certain forest microsites that is attributable to their dispersal and competitive abilities. Elevated microsites, especially decayed wood, act as seed beds for both ectomycorrhizal forest trees and fungi, thus affecting the succession of boreal forest ecosystems.

  11. Initial twig injury to high elevation Picea rubens in eastern North America caused by inadequate periderm formation

    SciTech Connect

    Evans, L.S. Brookhaven National Lab., Upton, NY ); Biesemeyer, P.T. )

    1988-09-01

    Apical dieback is the predominant injury symptomatology associated with growth declines of high elevation Picea rubens Sarg. trees in North America. Histological observation of uninjured tissues and of initial injury to tissues were made to understand the mechanism of injury to this species. Observations were made of hundreds of grab samples of apparently uninjured tissues and of uninjured twigs from trees growing on mountains of the Adirondack Mt., NY; Mount Mansfield, VT; Mt. Mitchell, NC; and Clingman's Dome, TN, from March 1985 to April 1986. The timing of developmental events varied markedly among buds of individual trees and among trees. Tissue necrosis occurred in twigs during their first overwintering period. Injured twigs with necrotic tissues had only one or two continuous or discontinuous phellem layers. In samples that exhibited initial injury, necrotic tissues consisted mostly of cortical cells and phloem subjacent to this meager periderm. Frequently, necrotic tissues developed initially near the bases of needles and at branch nodes (transition zone tissues with older twigs). In contrast, twigs of healthy appearance had two or more continuous phellem layers external to the phellogen.

  12. Divergent climate response on hydraulic-related xylem anatomical traits of Picea abies along a 900-m altitudinal gradient.

    PubMed

    Castagneri, Daniele; Petit, Giai; Carrer, Marco

    2015-12-01

    Climate change can induce substantial modifications in xylem structure and water transport capacity of trees exposed to environmental constraints. To elucidate mechanisms of xylem plasticity in response to climate, we retrospectively analysed different cell anatomical parameters over tree-ring series in Norway spruce (Picea abies L. Karst.). We sampled 24 trees along an altitudinal gradient (1200, 1600 and 2100 m above sea level, a.s.l.) and processed 2335 ± 1809 cells per ring. Time series for median cell lumen area (MCA), cell number (CN), tree-ring width (RW) and tree-ring-specific hydraulic conductivity (Kr) were crossed with daily temperature and precipitation records (1926-2011) to identify climate influence on xylem anatomical traits. Higher Kr at the low elevation site was due to higher MCA and CN. These variables were related to different aspects of intra-seasonal climatic variability under different environmental conditions, with MCA being more sensitive to summer precipitation. Winter precipitation (snow) benefited most parameters in all the sites. Descending the gradient, sensitivity of xylem features to summer climate shifted mostly from temperature to precipitation. In the context of climate change, our results indicate that higher summer temperatures at high elevations will benefit cell production and xylem hydraulic efficiency, whereas reduced water availability at lower elevations could negatively affect tracheids enlargement and thus stem capacity to transport water.

  13. [Responses of Picea likiangensis radial growth to climate change in the Small Zhongdian area of Yunnan Province, Southwest China].

    PubMed

    Zhao, Zhi-Jiang; Tan, Liu-Yi; Kang, Dong-Wei; Liu, Qi-Jing; Li, Jun-Qing

    2012-03-01

    Picea likiangensis (Franch. ) Pritz. primary forest is one of the dominant forest types in the Small Zhongdian area in Shangri-La County of Yunnan Province. In this paper, the responses of P. likiangensis tree-ring width to climate change were analyzed by dendrochronological methods, and the dendrochronology was built by using relatively conservative detrending negative exponential curves or linear regression. Correlation analysis and response function analysis were applied to explore the relationships between the residual chronology series (RES) and climatic factors at different time scales, and pointer year analysis was used to explain the reasons of producing narrow and wide rings. In the study area, the radial growth of P. likiangensis and the increasing air temperature from 1990 to 2008 had definite 'abruption'. The temperature and precipitation in previous year growth season were the main factors limiting the present year radial growth, and especially, the temperature in previous July played a negative feedback role in the radial growth, while the sufficient precipitation in previous July promoted the radial growth. The differences in the temperature variation and precipitation variation in previous year were the main reasons for the formation of narrow and wide rings. P. likiangensis radial growth was not sensitive to the variation of PDSI.

  14. Influence of climatic factors on tree-ring maximum latewood density of Picea schrenkiana in Xinjiang, China

    NASA Astrophysics Data System (ADS)

    Sun, Yu; Wang, Lili; Yin, Hong

    2016-03-01

    The influence of temperature and precipitation on maximum latewood density (MXD) was mainly discussed in this paper, based on the samples of Picea schrenkiana from the Manas River Basin, Xinjiang, China. The correlation analysis between MXD and instrumental records from the Shihezi Meteorological Station showed that the MXD was positively related to the mean maximum temperature throughout the growing season at high elevations. Comparatively, the ring-width at low altitudes was limited by the precipitation in May-June. The composite chronology by MXD sequences was highly correlated with the mean maximum temperature in July-August ( r = 0.54, p<0.001), which was then reconstructed by the composite chronology. The comparative analysis on the reconstructed temperatures, observed values, and drought indices ( Is) revealed that precipitation would affect MXD when the absolute value of Is was greater than 1.5σ (i.e., | Is|>2.5) in the period of 1953-2008 A.D. or close to 1.5σ for 2-3 consecutive years. The response characteristics are linked with the semiarid climate in the study area. In a single year or consecutive years of extreme dryness, the lack of precipitation would limit the thickening of latewood cell walls and thus impact the MXD. All in all, if a MXD chronology is aimed to reconstruct temperature history, the moisture conditions at the sampling site should be considered prudently.

  15. Growth, allocation and tissue chemistry of Picea abies seedlings affected by nutrient supply during the second growing season.

    PubMed

    Kaakinen, Seija; Jolkkonen, Annika; Iivonen, Sari; Vapaavuori, Elina

    2004-06-01

    One-year-old Norway spruce (Picea abies (L.) Karst.) seedlings were grown hydroponically in a growth chamber to investigate the effects of low and high nutrient availability (LN; 0.25 mM N and HN; 2.50 mM N) on growth, biomass allocation and chemical composition of needles, stem and roots during the second growing season. Climatic conditions in the growth chamber simulated the mean growing season from May to early October in Flakaliden, northern Sweden. In the latter half of the growing season, biomass allocation changed in response to nutrient availability: increased root growth and decreased shoot growth led to higher root/shoot ratios in LN seedlings than in HN seedlings. At high nutrient availability, total biomass, especially stem biomass, increased, as did total nonstructural carbohydrate and nitrogen contents per seedling. Responses of stem chemistry to nutrient addition differed from those of adult trees of the same provenance. In HN seedlings, concentrations of alpha-cellulose, hemicellulose and lignin decreased in the secondary xylem. Our results illustrate the significance of retranslocation of stored nutrients to support new growth early in the season when root growth and nutrient uptake are still low. We conclude that nutrient availability alters allocation patterns, thereby influencing the success of 2-year-old Norway spruce seedlings at forest planting sites.

  16. Identification of Free-Living and Particle-Associated Microbial Communities Present in Hadal Regions of the Mariana Trench.

    PubMed

    Tarn, Jonathan; Peoples, Logan M; Hardy, Kevin; Cameron, James; Bartlett, Douglas H

    2016-01-01

    Relatively few studies have described the microbial populations present in ultra-deep hadal environments, largely as a result of difficulties associated with sampling. Here we report Illumina-tag V6 16S rRNA sequence-based analyses of the free-living and particle-associated microbial communities recovered from locations within two of the deepest hadal sites on Earth, the Challenger Deep (10,918 meters below surface-mbs) and the Sirena Deep (10,667 mbs) within the Mariana Trench, as well as one control site (Ulithi Atoll, 761 mbs). Seawater samples were collected using an autonomous lander positioned ~1 m above the seafloor. The bacterial populations within the Mariana Trench bottom water samples were dissimilar to other deep-sea microbial communities, though with overlap with those of diffuse flow hydrothermal vents and deep-subsurface locations. Distinct particle-associated and free-living bacterial communities were found to exist. The hadal bacterial populations were also markedly different from one another, indicating the likelihood of different chemical conditions at the two sites. In contrast to the bacteria, the hadal archaeal communities were more similar to other less deep datasets and to each other due to an abundance of cosmopolitan deep-sea taxa. The hadal communities were enriched in 34 bacterial and 4 archaeal operational taxonomic units (OTUs) including members of the Gammaproteobacteria, Epsilonproteobacteria, Marinimicrobia, Cyanobacteria, Deltaproteobacteria, Gemmatimonadetes, Atribacteria, Spirochaetes, and Euryarchaeota. Sequences matching cultivated piezophiles were notably enriched in the Challenger Deep, especially within the particle-associated fraction, and were found in higher abundances than in other hadal studies, where they were either far less prevalent or missing. Our results indicate the importance of heterotrophy, sulfur-cycling, and methane and hydrogen utilization within the bottom waters of the deeper regions of the Mariana Trench

  17. Malaguana-Gadao Ridge: Identification and implications of a magma chamber reflector in the southern Mariana Trough

    NASA Astrophysics Data System (ADS)

    Becker, Nathan C.; Fryer, Patricia; Moore, Gregory F.

    2010-04-01

    Six-channel seismic reflection data reveal a magma chamber reflector beneath the Malaguana-Gadao Ridge, the southernmost segment of the spreading center in the Mariana Trough. For most of its length the spreading center in this active back-arc basin is morphologically similar to slow spreading mid-ocean ridges, having a deep central graben flanked by a zone of abyssal hill fabric. This southernmost segment, however, has a broad, smooth cross section, lacks a deep central graben, and is thus similar in morphology to fast spreading ridges (e.g., the East Pacific Rise). We identify a magma chamber at 1.5 s two-way travel time below the crest of the ridge. Observations from remotely operated vehicles along the ridge reveal not only fresh pillows, lobate, and sheet lava flows but also an abundance of volcaniclastic debris and intense hydrothermal activity. These observations, together with the "fast spreading" morphology of the ridge, suggest that this segment has a considerably higher magma supply than is typical in the Mariana Trough. We suggest that the volcanic arc or enhanced melting of a hydrated mantle is supplying volatile-rich magma as evidenced by a highly negative coefficient of reflection, -0.42, for this MCR and the presence of evolved, highly vesicular lava and volcaniclastic materials. The southeastern Mariana back-arc basin spreading ridge does not compare readily with mechanical models for global mid-ocean ridge data sets because of marked asymmetry in both volcanism and deformation that may be the consequence of slab-related geometry in this part of the convergent margin system.

  18. Identification of Free-Living and Particle-Associated Microbial Communities Present in Hadal Regions of the Mariana Trench

    PubMed Central

    Tarn, Jonathan; Peoples, Logan M.; Hardy, Kevin; Cameron, James; Bartlett, Douglas H.

    2016-01-01

    Relatively few studies have described the microbial populations present in ultra-deep hadal environments, largely as a result of difficulties associated with sampling. Here we report Illumina-tag V6 16S rRNA sequence-based analyses of the free-living and particle-associated microbial communities recovered from locations within two of the deepest hadal sites on Earth, the Challenger Deep (10,918 meters below surface-mbs) and the Sirena Deep (10,667 mbs) within the Mariana Trench, as well as one control site (Ulithi Atoll, 761 mbs). Seawater samples were collected using an autonomous lander positioned ~1 m above the seafloor. The bacterial populations within the Mariana Trench bottom water samples were dissimilar to other deep-sea microbial communities, though with overlap with those of diffuse flow hydrothermal vents and deep-subsurface locations. Distinct particle-associated and free-living bacterial communities were found to exist. The hadal bacterial populations were also markedly different from one another, indicating the likelihood of different chemical conditions at the two sites. In contrast to the bacteria, the hadal archaeal communities were more similar to other less deep datasets and to each other due to an abundance of cosmopolitan deep-sea taxa. The hadal communities were enriched in 34 bacterial and 4 archaeal operational taxonomic units (OTUs) including members of the Gammaproteobacteria, Epsilonproteobacteria, Marinimicrobia, Cyanobacteria, Deltaproteobacteria, Gemmatimonadetes, Atribacteria, Spirochaetes, and Euryarchaeota. Sequences matching cultivated piezophiles were notably enriched in the Challenger Deep, especially within the particle-associated fraction, and were found in higher abundances than in other hadal studies, where they were either far less prevalent or missing. Our results indicate the importance of heterotrophy, sulfur-cycling, and methane and hydrogen utilization within the bottom waters of the deeper regions of the Mariana Trench

  19. Epidemiological surveillance of amyotrophic lateral sclerosis and parkinsonism-dementia in the Commonwealth of the Northern Mariana Islands.

    PubMed

    Yanagihara, R T; Garruto, R M; Gajdusek, D C

    1983-01-01

    Amyotrophic lateral sclerosis (ALS) and parkinsonism-dementia (PD), two fatal neurological diseases of unknown cause, occur in high incidence among the Chamorro people of Guam, the largest and southernmost island within the Mariana archipelago. To reassess and extend our present epidemiological knowledge of these degenerative diseases in this focal geographical region, a systematic search for both disorders was conducted on the remaining inhabited islands of Rota, Tinian, Saipan, and the four remote islands of Anatahan, Alamagan, Pagan, and Agrihan within the Marianas chain. One case of ALS (on Saipan), 2 cases of PD (on Rota and Saipan), and 6 cases of parkinsonism without dementia (2 on Rota, 3 on Saipan, 1 on Tinian) were encountered among the approximately 17,000 inhabitants. No cases of either ALS, PD, or parkinsonism were found in the four remote Northern Islands. An additional 22 cases of ALS and 8 cases of PD were identified from reports of previous case-finding surveys, hospital records, and death certificates. Among Chamorros born on Rota, the average annual age-adjusted mortality rates of ALS per 100,000 population were 37.7 for the 15-year period 1956 to 1970 and 22.5 for the past decade, 1971 to 1980. Among Chamorros born on Saipan, the average annual mortality rates were 7.2 and 3.2 per 100,000, respectively, for the two periods. The mortality rates of PD were also significantly lower on Saipan than on Rota. In general, the age-adjusted mortality rates of ALS and PD on Rota were similar to those currently observed on Guam. Since the origins and current genotypic composition of Chamorros on all the Mariana Islands are indistinguishable, the strikingly lower mortality rates of ALS and PD on Saipan suggest that environmental factors are far more important than genetic factors in the pathogenesis of these diseases.

  20. ENVISAT SAR and InSAR Observations of Deformation and Crater Floor Elevation Change of Anatahan Volcano, Mariana Islands

    NASA Astrophysics Data System (ADS)

    Wicks, C.; Yarai, H.; Lu, Z.; Helz, R.

    2005-12-01

    Anatahan Volcano is a strato-volcano situated on a small 9 km by 3 km island in the Mariana Islands of the west Pacific Ocean. The volcano came to life with its first historical eruption in May 2003 ejecting a column of ash from the east crater up to 10 km into the atmosphere. The east crater is one of several craters that coalesce into a larger 5 km by 2 km caldera, the largest caldera in the volcanoes of the Mariana Islands. We will show results of a nascent InSAR study of Mariana volcanoes using European Space Agency (ESA) ENVISAT data. ESA began acquiring SAR data (IS2 and IS6 modes for both ascending and descending passes) over the Marianas Islands in November of 2004 at our request. Interferograms constructed from IS6 mode data display better coherence than those constructed from IS2 data. This coherence is reasonably well maintained from one acquisition to the next, and fairly well maintained over several months from Nov, 2004 to April, 2005. Limited explosive activity in late January and early February apparently did not destroy InSAR coherence through April 5. The largest of the eruptions, to date, occurred on April 5 (UT) about 12 hours after ESA acquired data over the island. Interferograms constructed using the April 5 image display a deformation field that is consistent with an inflating dike that trends SW from the east caldera to the south shore of the island. Since the April 5, 2005 eruption, there has been too much ash deposition and erosion to expect coherent interferograms for at least the near future. Because the inner walls of the east crater are steeper than the IS6 mode incident radar beams, which are incident about 40 degrees from vertical, we have used the change in radar shadow length to estimate the change in elevation of the crater floor. We found that the crater floor rose over 40 m between January 25 and March 1, 2005, and stayed at that level through the April 5 acquisition time. From this shadow length decrease, we have estimated a

  1. 8 CFR 233.6 - Aliens entering Guam or the Commonwealth of the Northern Mariana Islands pursuant to Title VII of...

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 8 Aliens and Nationality 1 2013-01-01 2013-01-01 false Aliens entering Guam or the Commonwealth of the Northern Mariana Islands pursuant to Title VII of Public Law 110-229, âConsolidated Natural Resources Act of 2008.â 233.6 Section 233.6 Aliens and Nationality DEPARTMENT OF HOMELAND SECURITY IMMIGRATION REGULATIONS CONTRACTS WITH...

  2. 8 CFR 233.6 - Aliens entering Guam or the Commonwealth of the Northern Mariana Islands pursuant to Title VII of...

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 8 Aliens and Nationality 1 2011-01-01 2011-01-01 false Aliens entering Guam or the Commonwealth of the Northern Mariana Islands pursuant to Title VII of Public Law 110-229, âConsolidated Natural Resources Act of 2008.â 233.6 Section 233.6 Aliens and Nationality DEPARTMENT OF HOMELAND SECURITY IMMIGRATION REGULATIONS CONTRACTS WITH...

  3. 8 CFR 233.6 - Aliens entering Guam or the Commonwealth of the Northern Mariana Islands pursuant to Title VII of...

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 8 Aliens and Nationality 1 2012-01-01 2012-01-01 false Aliens entering Guam or the Commonwealth of the Northern Mariana Islands pursuant to Title VII of Public Law 110-229, âConsolidated Natural Resources Act of 2008.â 233.6 Section 233.6 Aliens and Nationality DEPARTMENT OF HOMELAND SECURITY IMMIGRATION REGULATIONS CONTRACTS WITH...

  4. Vanadium Stable Isotope Variations in the Mariana Island Arc: Oxygen Fugacity Versus Magmatic Differentiation

    NASA Astrophysics Data System (ADS)

    Prytulak, J.; Elliott, T.; Halliday, A.; Kelley, K. A.; Nielsen, S. G.; Plank, T.; Schauble, E. A.

    2010-12-01

    A widely held view in igneous geochemistry is that the sub-arc mantle has elevated oxygen fugacity (fO2) compared to the upper mantle source of Mid-Ocean Ridge basalts (MORB). However, debate on the fO2 of the sub-arc mantle has arisen from examination of V/Sc ratios [1], which suggest no difference between the sub-arc mantle and the MORB source. This supposition is contrasted by recent μ-XANES determination of Fe3+/FeΣ in olivine-hosted melt inclusions [2], which supports the more traditional notion of an oxidized source for arc lavas. We have recently developed a method for high precision analyses of stable vanadium (V) isotope variations, able to resolve isotope fractionation to a precision of 0.15‰ 2sd [3, 4]. Theoretical calculations predict that stable V isotope fractionation should be robustly related to changes in fO2, with heavier isotopes favored in oxidizing conditions. Furthermore, V isotopes should be immune to alteration and late-stage degassing processes that could affect fO2 determined by Fe3+/FeΣ ratios. Therefore, examination of this new isotopic tracer in arc lavas may provide insight into the fO2 conditions of their source. Here we present the first stable V isotope measurements (reported as δ51V relative to a standard defined as 0‰) on subduction zone inputs (sediments, MORB) and outputs (arc lavas). We have focused initial efforts on well-characterized lavas from the Mariana central island province [5] and subducting sediment and underlying MORB from ODP Site 801, just outboard of the Mariana trench [6]. We find a surprisingly large, resolvable range in δ51V of the arc lavas of almost 0.8‰, which co-varies with SiO2, CaO, and V/Sc ratios. Co-variation of δ51V with SiO2 and CaO is suggestive of possible influence of clinopyroxene fractionation on the isotope composition. We explore the affects of magmatic differentiation and causes of δ51V inter-suite variability in arc lavas versus the δ51V signature of MORB. [1] Lee, C

  5. Topographic characteristics of four hydrothermal sites at the southern Mariana Trough

    NASA Astrophysics Data System (ADS)

    Yoshikawa, S.; Okino, K.; Asada, M.

    2011-12-01

    A high-resolution bathymetry is one of the essential information to clarify the broad overview of hydrothermal system. We here represent the geomorphological characteristics of four hydrothermal sites at the southern Mariana Trough, using the near-bottom swath mapping data that can produce 2 m grid spacing collected by SEABAT7125AUV on AUV Urashima during YK09-08 cruise, and dive observation data acquired by Shinkai6500 during YK10-11 cruise. In the study area, near 12°57'N, 143°37'E, the hydrothermal sites (Snail and Yamanaka site, Archaean site, Pika site) are located just on the active backarc spreading axis, the eastern foot of the axial high, and the top of an off-axis knoll about 5 km from the axis, respectively (e.g., Ishibashi et al., 2004; Kakegawa et al., 2008; Urabe et al., 2004), and these are aligned roughly perpendicular to the spreading axis. Each AUV survey was conducted within the area of about 1 km x 2 km. On-axis area is divided into two subareas based on their geological characteristics: 1) the area strongly deformed by many faults and fissures, and 2) the area with several mounds cut by fissures. The two hydrothermal sites (Snail and Yamanaka site) are distributed along the fissure in the latter area. The strike of faults and fissures and distribution of the mounds are approximately parallel to the spreading axis. The height of the mounds ranges from 5 to 30 m, and its diameter is 250 to 320 m. They have comparatively flat tops and gentle slopes, indicating dome like morphology. The Archean site is located on the top of single mound. The mound is conical in shape, whose diameter is 250 to 300 m and its height is 50 to 100 m. Numerous ridge lines and several chimney-like structures (up to about 6 m high) are formed on the top and slope of the mound. The age of the mound is younger than that of surrounding rugged seafloor because of the undeformed mound morphology. The off-axis knoll consists of two peaks. The Pika site is located on its

  6. The eruption of Mount Pagan volcano, Mariana Islands, 15 May 1981

    USGS Publications Warehouse

    Banks, N.G.; Koyanagi, R.Y.; Sinton, J.M.; Honma, K.T.

    1984-01-01

    A major explosive eruption occurred 15 May 1981 at Mount Pagan Volcano, the larger of two historic eruptive centers on Pagan Island, Mariana Islands. The eruption was preceded by increased numbers of locally felt earthquakes beginning in late March or early April and by new ground cracks, new sublimates, and increased gas emissions. A swarm of felt earthquakes began at 0745h (local time = UCT+10 hours) 15 May, and at 0915 h, closely following a loud sonic boom, a strong plinian column issued from the volcano. The high-altitude ash cloud (at least 13.5 km) travelled south-southeast, but ash and scoria deposits were thickest (> 2 m) in the NW sector of the island because of the prevailing low-altitude southeasterly winds. The early activity of 15 May probably involved magmatic eruption along a fissure system oriented about N10??E. However, the eruption became hydromagmatic, possibly within minutes, and was largely restricted to three long-lived vents. The northernmost of these built a substantial new scoria-ash cinder cone. Flows and air-fall deposits, consisting almost entirely of juvenile material, exceeded 105 ?? 106 m3 in volume (75 ?? 106 m3 of magma) on land and at least 70-100 ?? 606 m3 at sea. An unknown volume was carried away by stratospheric winds. Lithic blocks and juvenile bombs as large as 1 m in diameter were thrown more than 2 km from the summit, and evidence for base-surge was observed in restricted corridors as low as 200 m elevation on the north and south slopes of the volcano. Neither of these events resulted in serious injuries to the 54 residents of the island, nor did the eruption produce serious chemical hazards in their water supply. Weak eruptions occurred during the ensuing month, and some of these were monitored by ground observations, seismic monitoring, and deformation studies. Precursory seismicity and possibly deformation occurred with some of the observed eruptions. More vigorous eruptions were reported by visiting residents in late

  7. The May 2010 submarine eruption from South Sarigan seamount, Northern Mariana Islands

    NASA Astrophysics Data System (ADS)

    McGimsey, R. G.; Neal, C. A.; Searcy, C. K.; Camacho, J. T.; Aydlett, W. B.; Embley, R. W.; Trusdell, F.; Paskievitch, J. F.; Schneider, D. J.

    2010-12-01

    A sudden submarine explosive eruption occurred on May 29, 2010, from a seamount south of Sarigan Island in the Northern Mariana Islands, propelling a diffuse steam and ash cloud to high altitude. Pre-eruptive seismicity was recorded in early April by stations located on Sarigan and Anatahan Island, 42 km to the south, and indicated a source ~12-16 km south of Sarigan. On May 27-28, a change in seismicity—the appearance of tremor-like waveforms—may have marked the onset of volcanic activity. Also on May 27, an elongate patch of discolored ocean water and possible light-colored floating debris about 8-11 km south of Sarigan was observed from a helicopter. This material was likely produced during low-intensity eruptive activity, and an Information Statement from the Commonwealth of the Northern Mariana Islands (CNMI) Emergency Management Office (EMO) and USGS issued at 2353 UTC May 28 described the observation. The Guam Weather Forecast Office of the National Weather Service reported that the area of discoloration, visible on satellite images at 2313 and 2330 UTC on May 28, was about 10 km2, about twice the size of Sarigan Island. Pulses of tremor merged into a nearly continuous signal by 0305 UTC on May 29, lasting for ~4.5 hours followed by nearly 4.5 hours of quiescence. The EMO issued a declaration closing the region south of Sarigan to all local boating traffic and issued an advisory to aircraft. The explosive onset of the main plume-producing event occurred at ~1148 UTC as confirmed by seismic records on Anatahan Island, with the strongest phase ending ~1200 UTC. Soon after, the Washington Volcanic Ash Advisory Center reported an eruption cloud reaching an estimated 40,000 feet (12 km) ASL that diminished rapidly on satellite imagery suggesting it was water-vapor dominated. Winds carried the cloud southwest over Guam, and although no ash fall was reported, the cloud was visible and was detected in Aura/OMI aerosol index imagery. Biologists on Sarigan Island

  8. What caused the population decline of the Bridled White-eye on Rota, Mariana Islands?

    USGS Publications Warehouse

    Fancy, Steven G.; Snetsinger, Thomas J.

    2001-01-01

    The Bridled White-eye (Zosterops conspicillatus rotensis) was Once thought to be common and widespread on Rota, Commonwealth of the Northern Mariana Islands, but is now restricted to several patches of native limestone forest in and adjacent to the Sabana region. Surveys conducted in 1990 indicated that the population had declined by 87% between 1982 and 1990 for unknown reasons. The low density and restricted habitat association of the Bridled White-eye on Rota contrasts with the situation on Saipan, Tinian, Agiguan, and formerly on Guam, where the Bridled White-eye is the most common forest bird and occurs at all elevations and in all habitat types. We surveyed the entire range of the Rota Bridled White-eye in 1996 to estimate its current numbers and distribution. We also reviewed existing information on the white-eye and evaluated potential causes of its decline, including predation by Black Drongos (Dicrurus macrocercus), rats (Rattus spp.), and the brown tree snake Boiga irregularis); pesticides; avian disease; and habitat loss and alteration. We found that 94% of the extant population of 1,165 white-eyes on Rota was restricted to four patches of old-growth, native limestone forest covering only 259 ha. We believe that the population decline and current localized distribution is primarily a result of habitat changes due to agricultural development and typhoons, but the absence of white-eyes from several stands of native forest above 200 m remains unexplained. The Rota white-eye may be a different species from white-eyes found on Saipan, Tinian, Agiguan, and Guam, with different habitat preferences.

  9. Imaging of CO2 bubble plumes above an erupting submarine volcano, NW Rota-1, Mariana Arc

    NASA Astrophysics Data System (ADS)

    Chadwick, William W.; Merle, Susan G.; Buck, Nathaniel J.; Lavelle, J. William; Resing, Joseph A.; Ferrini, Vicki

    2014-11-01

    Rota-1 is a submarine volcano in the Mariana volcanic arc located ˜100 km north of Guam. Underwater explosive eruptions driven by magmatic gases were first witnessed there in 2004 and continued until at least 2010. During a March 2010 expedition, visual observations documented continuous but variable eruptive activity at multiple vents at ˜560 m depth. Some vents released CO2 bubbles passively and continuously, while others released CO2 during stronger but intermittent explosive bursts. Plumes of CO2 bubbles in the water column over the volcano were imaged by an EM122 (12 kHz) multibeam sonar system. Throughout the 2010 expedition numerous passes were made over the eruptive vents with the ship to document the temporal variability of the bubble plumes and relate them to the eruptive activity on the seafloor, as recorded by an in situ hydrophone and visual observations. Analysis of the EM122 midwater data set shows: (1) bubble plumes were present on every pass over the summit and they rose 200-400 m above the vents but dissolved before they reached the ocean surface, (2) bubble plume deflection direction and distance correlate well with ocean current direction and velocity determined from the ship's acoustic doppler current profiler, (3) bubble plume heights and volumes were variable over time and correlate with eruptive intensity as measured by the in situ hydrophone. This study shows that midwater multibeam sonar data can be used to characterize the level of eruptive activity and its temporal variability at a shallow submarine volcano with robust CO2 output.

  10. Long Term Seismic Observation in Mariana by OBSs : Double Seismic Zone and Upper Mantle Structure

    NASA Astrophysics Data System (ADS)

    Shiobara, H.; Sugioka, H.; Mochizuki, K.; Oki, S.; Kanazawa, T.; Fukao, Y.; Suyehiro, K.

    2005-12-01

    In order to obtain the deep arc structural image of Mariana, a large-scale seismic observation by using 58 long-term ocean bottom seismometers (LTOBS) had been performed from June 2003 until April 2004, which is a part of the MARGINS program funded by the NSF. Prior to this observation, a pilot long-term seismic array observation was conducted in the same area by using 10 LTOBSs from Oct. 2001 until Feb. 2003. At that time, 8 LTOBSs were recovered but one had no data. Recently, 2 LTOBSs, had troubles in the releasing, were recovered by the manned submersible (Shinkai 6500, Jamstec) for the research of the malfunction in July 2005. By using all 9 LTOBS's data, those are about 11 months long, hypocenter determination was performed and more than 3000 local events were found. Even with the 1D velocity structure based on the iasp91 model, double seismic zones and a systematic shift of epicenters between the PDE and this study were observed. To investigate the detail of hypocenter distribution and the 3D velocity structure, the DD inversion (tomoDD: Zhang and Thurber, 2003) was applied for this data set with the 1D structure initial model except for the crust, which has been surveyed by using a dense airgun-OBS system (Takahashi et al., 2003). The result of relocated hypocenters shows clear double seismic zones until about 200 km depth, a high activity area around the fore-arc serpentine sea-mount, the Big Blue, and a lined focuses along the current ridge axis in the back-arc basin, and the result of the tomography shows a image of subducting slab and a low-Vs region below the same sea-mount mentioned. The wedge mantle structure was not clearly resolved due to the inadequate source-receiver coverage, which will be done in the recent experiment.

  11. Long Term Seismic Observation in Mariana by OBSs : Results of the DD inversion

    NASA Astrophysics Data System (ADS)

    Shiobara, H.; Sugioka, H.; Mochizuki, K.; Oki, S.; Kanazawa, T.; Fukao, Y.; Suyehiro, K.

    2004-12-01

    In order to obtain the deep arc structural image of Mariana, a large-scale seismic observation by using 58 long-term ocean bottom seismometers (LTOBS) had been performed from June 2003 until April 2004, which is a part of the MARGINS program funded by the NSF. Prior to this observation, a pilot long-term seismic array observation was conducted in the same area by using 10 LTOBSs from Oct. 2001 until Feb. 2003. By using seven LTOBS's data, those are about 11 months long, hypocenter determination was performed at first and more than 3000 local events were found, although the PDE list contains only 59. A 1D velocity structure based on the iasp91 model was used, and a systematic shift of epicenters between the PDE list and this study was seen. To investigate the detail of hypocenter distribution and the 3D velocity structure, the DD inversion (tomoDD: Zhang and Thurber, 2003) was applied for this data set with a 1D structure initial model except for the crust, which has been surveyed by using a dense airgun-OBS system (Takahashi et al., 2003). The result of relocated hypocenters shows double seismic zones until about 200km depth and a lined focuses along the current ridge axis in the back-arc basin, and the result of the tomographic inversion shows a image of subducting slab and a low-Vs region below the Pagan island erupted in 1981 at 80km depth. The mantle structure beneath the back-arc basin was not clearly resolved due to the inadequate source-receiver coverage, which is cleared in the recent experiment.

  12. In Situ Observations of Dissolved Manganese in Hydrothermal Vent Plumes at Mariana Trough.

    NASA Astrophysics Data System (ADS)

    Okamura, K.; Yanai, K.; Sohrin, Y.; Ishibashi, J.; Watanabe, M.; Ura, T.

    2004-12-01

    We studied for hydrothermal plumes in Mariana Trough by using in situ Mn-Fe analyzers (GAMOS-II). GAMOS-II (Geochemical Anomalies MOnitoring system) is an in-situ chemical analyzer used to detect manganese and/or iron anomalies in neutrally buoyant plumes and to map distributions in bottom seawater over vent fields. During TN167 (ROV ROPOS / R/V Thomas G Thompson) cruise, GAMOS-II measurements were conducted for plume observation at the Yamanaka and Fryer sites. GAMOS-II was attached on the sampling stage of the ROPOS at dive #'777. ROPOS arrived at the bottom at 0:50, and left the bottom at 10:55. Active manganese and temperature anomalies were detected around 2:00 - 5:00 and 7:00 - 11:00, when the ROPOS passed through hydrothermally active areas. The anomaly of temperature and manganese concentration was observed coincidentally, but the relation ship is not consistently proportional. Wide variation in Mn vs. temperature ratio implies diversity between geochemical flux and heat flux depending on the type of venting in the hydrothermal sites. During KH-04-02 Leg2 (AUV r2D4 / R/V Hakuho-Maru) cruise, GAMOS-II measurements were also conducted for plume observation at NW ROTA #1 seamount. GAMOS-II was attached in the AUV r2D4 with CTD. During four successive dives, the fine structure of hydrothermal plumes changed drastically, probably reflecting temporal variation of hydrothermal activity. Continuous sampling by using GAMOS-II was also done successfully. We will also discuss about the data of this continuous sampling.

  13. Spawning Sites of the Japanese Eel in Relation to Oceanographic Structure and the West Mariana Ridge

    PubMed Central

    Aoyama, Jun; Watanabe, Shun; Miller, Michael J.; Mochioka, Noritaka; Otake, Tsuguo; Yoshinaga, Tatsuki; Tsukamoto, Katsumi

    2014-01-01

    The Japanese eel, Anguilla japonica, spawns within the North Equatorial Current that bifurcates into both northward and southward flows in its westward region, so its spawning location and larval transport dynamics seem important for understanding fluctuations in its recruitment to East Asia. Intensive research efforts determined that Japanese eels spawn along the western side of the West Mariana Ridge during new moon periods, where all oceanic life history stages have been collected, including eggs and spawning adults. However, how the eels decide where to form spawning aggregations is unknown because spawning appears to have occurred at various latitudes. A salinity front formed from tropical rainfall was hypothesized to determine the latitude of its spawning locations, but an exact spawning site was only found once by collecting eggs in May 2009. This study reports on the collections of Japanese eel eggs and preleptocephali during three new moon periods in June 2011 and May and June 2012 at locations indicating that the distribution of lower salinity surface water or salinity fronts influence the latitude of spawning sites along the ridge. A distinct salinity front may concentrate spawning south of the front on the western side of the seamount ridge. It was also suggested that eels may spawn at various latitudes within low-salinity water when the salinity fronts appeared unclear. Eel eggs were distributed within the 150–180 m layer near the top of the thermocline, indicating shallow spawning depths. Using these landmarks for latitude (salinity front), longitude (seamount ridge), and depth (top of the thermocline) to guide the formation of spawning aggregations could facilitate finding mates and help synchronize their spawning. PMID:24551155

  14. Ten years of satellite observations reveal highly variable sulphur dioxide emissions at Anatahan Volcano, Mariana Islands

    NASA Astrophysics Data System (ADS)

    McCormick, Brendan; Popp, Christoph; Andrews, Benjamin; Cottrell, Elizabeth

    2015-07-01

    Satellite remote sensing enables continuous multiyear observations of volcanic activity in remote settings. Anatahan (Mariana Islands) is a remote volcano in the western North Pacific. Available ground-based measurements of sulphur dioxide (SO2) gas emissions at Anatahan place it among thelargest volcanic SO2 sources worldwide. These ground-based measurements, however, are restricted to eruptive intervals. Anatahan's activity since 2003 has been dominated temporally by prolonged periods of quiescence. Using 10 years of satellite observations from OMI, AIRS, SCIAMACHY, and GOME-2, we report highly variable SO2 emissions within and between eruptive and quiescent intervals at Anatahan. We find close correspondence between levels of activity reported at the volcano and levels of SO2 emissions detected from space. Eruptive SO2 emission rates have a mean value of ˜6400 t d-1, but frequently are in excess of 20,000 t d-1. Conversely, SO2 emissions during quiescent intervals are below the detection limit of space-based sensors and therefore are not likely to exceed ˜300 t d-1. We show that while Anatahan occupies a quiescent state for 85% of the past 10 years, only ˜15% of total SO2 emissions over this interval occur during quiescence, with the remaining ˜85% released in short duration but intense syn-eruptive degassing. We propose that the integration of multiyear satellite data sets and activity histories are a powerful complement to targeted ground-based campaign measurements in better describing the long-term degassing behavior of remote volcanoes.

  15. Vertically migrating micronekton and macrozooplankton communities around Guam and the Northern Mariana Islands

    NASA Astrophysics Data System (ADS)

    Suntsov, Andrey; Domokos, Réka

    2013-01-01

    The distribution, biomass, and assemblages of vertically migrating micronekton/macrozooplankton were studied in relation to oceanographic conditions around Guam and the adjacent Northern Mariana Islands during Spring 2010, using 3-m2 Isaacs-Kidd Midwater Trawl (IKMT). The study area was located within the oligotrophic waters of the westward flowing North Equatorial Current (NEC). However, southern stations of the survey were situated close to the northern boundary of the more productive North Equatorial Countercurrent (NECC), where we observed the highest biomass, abundance, species richness, and diversity of pelagic organisms. Overall, we recorded 85 species from 20 families of mostly mesopelagic species in the area, with lanternfishes (Myctophidae-40 species) and dragonfishes (Stomiidae-18 species) being the most taxonomically diverse groups. Three genera of mesopelagic shrimps, Sergestes, Janicella and Sergia, dominated the decapod crustacean component of the micronekton community numerically and by biomass, while the contribution from cephalopods was relatively minor. Assemblages of major micronekton/macrozooplankton groups, based on biomass and abundance showed principal changes with latitude. However, the classification and ordination analysis, based on taxonomically resolved taxa (fishes and decapod shrimps), indicated additional zonal variation, with areas east and west of the island chain showing different community structure. The mean total micronekton biomass for the area near the productive boundary region between the NEC and NECC was 5.8 mg/m3, with a mean biomass of 1.2 mg/m3 obtained for stations in the oligotrophic NEC area. The corresponding biomass of mesopelagic fishes was 0.88 mg/m3 and 0.24 mg/m3 for these two areas, respectively. We reviewed and compared the available information on the quantitative distribution of midwater fish biomass in the western tropical Pacific and outlined major patterns of variation in the equatorial Pacific in

  16. Geochemical evidence for sundering of the West Mariana arc in miocene ash from the Parece Vela Basin

    NASA Astrophysics Data System (ADS)

    Warner, Russell J.; Flower, Martin F. J.; Rodolfo, Kelvin S.

    1987-10-01

    Glass and mineral fragments from discrete volcanic ash layers were sampled from DSDP/IPOD Site 450 in the Parece Vela Basin, Philippine Sea and analyzed by electron microprobe. The ashes are interpreted as eruptive products of the adjacent West Mariana arc system between 25 and 14 Ma B.P., and have compositions between basaltic andesite and rhyolite, and rarely, boninite. 'Continuous' chemical trends appear to reflect mixing of mafic and silicic magmas. 'Discontinuous' trends between these end-members are relatively few, and are consistent with 'liquid lines' produced by fractional crystallization. Andesitic tephra become progressively richer in MgO and CaO through the middle Miocene, while boninite appears towards the end of the sequence, between 14 and 15 Ma B.P. Coeval rhyolitic glasses become richer in K 2O and Na 2O, with maximum concentrations at about 15 Ma B.P. Chronologic changes in fractionation type and composition of parent magmas are interpreted to reflect the subaerial volcanic evolution of the West Mariana arc. The appearance of boninite is believed to signal early stages of arc sundering, and corresponds temporally with regional uplift of the sea floor above the carbonate compensation depth, precursor to a new pulse of back-arc spreading.

  17. Altered HBK3 expression affects glutathione and ascorbate metabolism during the early phases of Norway spruce (Picea abies) somatic embryogenesis.

    PubMed

    Belmonte, Mark F; Stasolla, Claudio

    2009-10-01

    Plant homeobox genes play an important role in plant development, including embryogenesis. Recently, the function of a class I homeobox of knox 3 gene, HBK3, has been characterized in the conifer Picea abies (L.) Karst (Norway spruce) [8]. During somatic embryogenesis, expression of HBK3 is required for the proper differentiation of proembryogenic masses into somatic embryos. This transition, fundamental for the overall embryogenic process, is accelerated in sense lines over-expressing HBK3 (HBK3-S) but precluded in antisense lines (HBK3-AS) where the expression of this gene is experimentally reduced. Altered HBK3 expression resulted in major changes of ascorbate and glutathione metabolism. During the initial phases of embryogeny the level of reduced GSH was higher in the HBK3-S lines compared to their control counterpart. An opposite profile was observed for the HBK3-AS lines where the glutathione redox state, i.e. GSH/GSH + GSSG, switched towards its oxidized form, i.e. GSSG. Very similar metabolic fluctuations were also measured for ascorbate, especially during the transition of proembryogenic masses into somatic embryos (7 days into hormone-free medium). At this stage the level of reduced ascorbate (ASC) in the HBK3-AS lines was about 75% lower compare to the untransformed line causing a switch of the ascorbate redox state, i.e. ASC/ASC + DHA + AFR, towards its oxidized forms, i.e. DHA + AFR. Changes in activities of several ascorbate and glutathione redox enzymes, including dehydroascorbate reductase (EC 1.8.5.1), ascorbate free radical reductase (EC 1.6.5.4) and glutathione reductase (GR; EC 1.6.4.2) were responsible for these metabolic differences. Data presented here suggest that HBK3 expression might regulate somatic embryo yield through alterations in glutathione and ascorbate metabolism, which have been previously implicated in controlling embryo development and maturation both in vivo and in vitro.

  18. Effect of season, needle age and elevated CO2 concentration on photosynthesis and Rubisco acclimation in Picea abies.

    PubMed

    Urban, Otmar; Hrstka, Miroslav; Zitová, Martina; Holišová, Petra; Sprtová, Mirka; Klem, Karel; Calfapietra, Carlo; De Angelis, Paolo; Marek, Michal V

    2012-09-01

    While downward photosynthetic acclimation in response to elevated CO(2) (EC) is frequently accompanied by reduction in Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase), the exact mechanism behind this decrease and its dynamics are not well understood. We comprehensively studied Rubisco adjustment to EC in coniferous Picea abies using an electrophoretic (protein content), spectrophotometric (initial (RA(initial)) and total (RA(total)) in vitro Rubisco activities), and gas-exchange (maximum carboxylation activity in vivo (V(Cmax))) techniques. With respect to differing carbon sink strength and nitrogen remobilization, we hypothesized greater acclimation of photosynthesis in one-year-old as compared to current-year needles and at the end than at the beginning of the vegetation season. EC treatment led to a decrease in V(Cmax) values in current-year needles, but the ribulose-1,5-bisphosphate (RuBP)-limited rate of photosynthesis (J(max)) remained unaffected. Indeed, both V(Cmax) and J(max) were reduced by the EC treatment in one-year-old needles. The extent of photosynthetic acclimation in EC plants did not increase, however, during the vegetation season. EC decreased the activation state of Rubisco (RA(initial)/RA(total)) by 16% and 5% in current-year and one-year-old needles, respectively (averaged over the growing season). While during spring (short-term effect) EC treatment did not influence the Rubisco content per unit leaf area and decreased its specific activity (activity per unit Rubisco mass) in both needle age classes studied, exposure to EC during the entire vegetation season tended to reduce the Rubisco content while increasing its specific activity. Irrespective of CO(2) treatment and needle age, a hyperbolic-decay relationship was observed between Rubisco-specific activity and its content.

  19. The joint influence of photoperiod and temperature during growth cessation and development of dormancy in white spruce (Picea glauca).

    PubMed

    Hamilton, Jill A; El Kayal, Walid; Hart, Ashley T; Runcie, Daniel E; Arango-Velez, Adriana; Cooke, Janice E K

    2016-11-01

    Timely responses to environmental cues enable the synchronization of phenological life-history transitions essential for the health and survival of north-temperate and boreal tree species. While photoperiodic cues will remain persistent under climate change, temperature cues may vary, contributing to possible asynchrony in signals influencing developmental and physiological transitions essential to forest health. Understanding the relative contribution of photoperiod and temperature as determinants of the transition from active growth to dormancy is important for informing adaptive forest management decisions that consider future climates. Using a combination of photoperiod (long = 20 h or short = 8 h day lengths) and temperature (warm = 22 °C/16 °C and cool = 8 °C/4 °C day/night, respectively) treatments, we used microscopy, physiology and modeling to comprehensively examine hallmark traits of the growth-dormancy transition-including bud formation, growth cessation, cold hardiness and gas exchange-within two provenances of white spruce [Picea glauca (Moench) Voss] spanning a broad latitude in Alberta, Canada. Following exposure to experimental treatments, seedlings were transferred to favorable conditions, and the depth of dormancy was assessed by determining the timing and ability of spruce seedlings to resume growth. Short photoperiods promoted bud development and growth cessation, whereas longer photoperiods extended the growing season through the induction of lammas growth. In contrast, cool temperatures under both photoperiodic conditions delayed bud development. Photoperiod strongly predicted the development of cold hardiness, whereas temperature predicted photosynthetic rates associated with active growth. White spruce was capable of attaining endodormancy, but its release was environmentally determined. Dormancy depth varied substantially across experimental treatments suggesting that environmental cues experienced within one season could affect growth

  20. Impact of Heterobasidion root-rot on fine root morphology and associated fungi in Picea abies stands on peat soils.

    PubMed

    Gaitnieks, Talis; Klavina, Darta; Muiznieks, Indrikis; Pennanen, Taina; Velmala, Sannakajsa; Vasaitis, Rimvydas; Menkis, Audrius

    2016-07-01

    We examined differences in fine root morphology, mycorrhizal colonisation and root-inhabiting fungal communities between Picea abies individuals infected by Heterobasidion root-rot compared with healthy individuals in four stands on peat soils in Latvia. We hypothesised that decreased tree vitality and alteration in supply of photosynthates belowground due to root-rot infection might lead to changes in fungal communities of tree roots. Plots were established in places where trees were infected and in places where they were healthy. Within each stand, five replicate soil cores with roots were taken to 20 cm depth in each root-rot infected and uninfected plot. Root morphological parameters, mycorrhizal colonisation and associated fungal communities, and soil chemical properties were analysed. In three stands root morphological parameters and in all stands root mycorrhizal colonisation were similar between root-rot infected and uninfected plots. In one stand, there were significant differences in root morphological parameters between root-rot infected versus uninfected plots, but these were likely due to significant differences in soil chemical properties between the plots. Sequencing of the internal transcribed spacer of fungal nuclear rDNA from ectomycorrhizal (ECM) root morphotypes of P. abies revealed the presence of 42 fungal species, among which ECM basidiomycetes Tylospora asterophora (24.6 % of fine roots examined), Amphinema byssoides (14.5 %) and Russula sapinea (9.7 %) were most common. Within each stand, the richness of fungal species and the composition of fungal communities in root-rot infected versus uninfected plots were similar. In conclusion, Heterobasidion root-rot had little or no effect on fine root morphology, mycorrhizal colonisation and composition of fungal communities in fine roots of P. abies growing on peat soils.

  1. Carbon dioxide concentration and nitrogen input affect the C and N storage pools in Amanita muscaria-Picea abies mycorrhizae.

    PubMed

    Turnau, K; Berger, A; Loewe, A; Einig, W; Hampp, R; Chalot, M; Dizengremel, P; Kottke, I

    2001-02-01

    We studied the influence of elevated atmospheric CO2 concentration ([CO2]) on the vacuolar storage pool of nitrogen-containing compounds and on the glycogen pool in the hyphal sheath of Amanita muscaria (L. ex Fr.) Hooker-Picea abies L. Karst. mycorrhizae grown with two concentrations of ammonium in the substrate. Mycorrhizal seedlings were grown in petri dishes on agar containing 5.3 or 53 mg N l(-1) and exposed to 350 or 700 microl CO2 l(-1) for 5 or 7 weeks, respectively. Numbers and area of nitrogen-containing bodies in the vacuoles of the mycorrhizal fungus were determined by light microscopy linked to an image analysis system. The relative concentration of nitrogen in the vacuolar bodies was measured by electron energy loss spectroscopy (EELS). Glycogen stored in the cytosol was determined at the ultrastructural level by image analysis after staining the sections (PATAg test). Shoot dry weight, net photosynthesis and relative amounts of N in vacuolar bodies were greater at the higher N and CO2 concentrations. The numbers and areas of vacuolar N-containing bodies were significantly greater at the higher N concentration only at ambient [CO2]. In the same treatment the percentage of hyphae containing glycogen declined to nearly zero. We conclude that, in the high N/low [CO2] treatment, the mycorrhizal fungus had an insufficient carbohydrate supply, partly because of increased amino acid synthesis by the non-mycorrhizal rootlets. When [CO2] was increased, the equilibrium between storage of glycogen and N-containing compounds was reestablished.

  2. The influence of Ceratocystis polonica inoculation and methyl jasmonate application on terpene chemistry of Norway spruce, Picea abies.

    PubMed

    Zhao, Tao; Krokene, Paal; Björklund, Niklas; Långström, Bo; Solheim, Halvor; Christiansen, Erik; Borg-Karlson, Anna-Karin

    2010-08-01

    Constitutive and inducible terpene production is involved in conifer resistance against bark beetles and their associated fungi. In this study 72 Norway spruce (Picea abies) were randomly assigned to methyl jasmonate (MJ) application, inoculation with the bluestain fungus Ceratocystis polonica, or no-treatment control. We investigated terpene levels in the stem bark of the trees before treatment, 30 days and one year after treatment using GC-MS and two-dimensional GC (2D-GC) with a chiral column, and monitored landing and attack rates of the spruce bark beetle, Ips typographus, on the trees by sticky traps and visual inspection. Thirty days after fungal inoculation the absolute amount and relative proportion of (+)-3-carene, sabinene, and terpinolene increased and (+)-alpha-pinene decreased. Spraying the stems with MJ tended to generally increase the concentration of most major terpenes with minor alteration to their relative proportions, but significant increases were only observed for (-)-beta-pinene and (-)-limonene. Fungal inoculation significantly increased the enantiomeric ratio of (-)-alpha-pinene and (-)-limonene 1 month after treatment, whereas MJ only increased that of (-)-limonene. One year after treatment, both MJ and fungal inoculation increased the concentration of most terpenes relative to undisturbed control trees, with significant changes in (-)-beta-pinene, (-)-beta-phellandrene and some other compounds. Terpene levels did not change in untreated stem sections after treatment, and chemical induction by MJ and C. polonica thus seemed to be restricted to the treated stem section. The enantiomeric ratio of (-)-alpha-pinene was significantly higher and the relative proportions of (-)-limonene were significantly lower in trees that were attractive to bark beetles compared to unattractive trees. One month after fungal inoculation, the total amount of diterpenes was significantly higher in putative resistant trees with shorter lesion lengths than in

  3. A possible biochemical basis for fructose-induced inhibition of embryo development in Norway spruce (Picea abies).

    PubMed

    Businge, Edward; Egertsdotter, Ulrika

    2014-06-01

    Sugars play an important role in various physiological processes during plant growth and development; however, the developmental roles and regulatory functions of hexoses other than glucose are still largely unclear. Recent studies suggest that blocked embryo development in Norway spruce (Picea abies (L.) Karst) is associated with accumulation of fructose. In the present study, the potential biochemical regulatory mechanism of glucose and fructose was studied during development of somatic embryos of Norway spruce from pro-embryogenic masses to mature embryos. The changes in protein fluorescence, a marker of the Maillard reaction, were monitored in two cell lines of Norway spruce that were grown on media containing sucrose (control), glucose or fructose. Manual time-lapse photography showed that growth of embryogenic cultures on medium containing sucrose was characterized by normal development of mature embryos whereas the embryogenic cultures that were grown on media containing glucose or fructose did not develop mature embryos. The biochemical analyses of embryogenic samples collected during embryo development showed that: (i) the content of glucose and fructose in the embryogenic cultures increased significantly during growth on each medium, respectively; (ii) the accumulation of Maillard products in the embryogenic cultures was highly correlated with the endogenous content of fructose but not glucose; and (iii) the embryogenic cultures grown on fructose displayed the highest protein carbonyl content and DNA damage whereas the highest content of glutathione was recorded in the embryogenic cultures that had grown on sucrose. Our data suggest that blocked development of embryos in the presence of fructose may be associated with the Maillard reaction.

  4. Growth of mycorrhizal jack pine (Pinus banksiana) and white spruce (Picea glauca) seedlings planted in oil sands reclaimed areas.

    PubMed

    Onwuchekwa, Nnenna E; Zwiazek, Janusz J; Quoreshi, Ali; Khasa, Damase P

    2014-08-01

    The effectiveness of ectomycorrhizal inoculation at the tree nursery seedling production stage on growth and survival was examined in jack pine (Pinus banksiana) and white spruce (Picea glauca) planted in oil sands reclamation sites. The seedlings were inoculated with Hebeloma crustuliniforme strain # UAMH 5247, Suillus tomentosus strain # UAMH 6252, and Laccaria bicolor strain # UAMH 8232, as individual pure cultures and in combinations. These treatments were demonstrated to improve salinity resistance and water uptake in conifer seedlings. The field responses of seedlings to ectomycorrhizal inoculation varied between plant species, inoculation treatments, and measured parameters. Seedling inoculation resulted in higher ectomycorrhizal colonization rates compared with non-inoculated control, which had also a relatively small proportion of roots colonized by the nursery contaminant fungi identified as Amphinema byssoides and Thelephora americana. Seedling inoculation had overall a greater effect on relative height growth rates, dry biomass, and stem volumes in jack pine compared with white spruce. However, when examined after two growing seasons, inoculated white spruce seedlings showed up to 75% higher survival rates than non-inoculated controls. The persistence of inoculated fungi in roots of planted seedlings was examined at the end of the second growing season. Although the inoculation with H. crustuliniforme triggered growth responses, the fungus was not found in the roots of seedlings at the end of the second growing season suggesting a possibility that the observed growth-promoting effect of H. crustuliniforme may be transient. The results suggest that the inoculation of conifer seedlings with ectomycorrhizal fungi could potentially be carried out on a large scale in tree nurseries to benefit postplanting performance in oil sands reclamation sites. However, these practices should take into consideration the differences in responses between the different

  5. Shoot-level terpenoids emission in Norway spruce (Picea abies) under natural field and manipulated laboratory conditions.

    PubMed

    Esposito, Raffaela; Lusini, Ilaria; Kristýna Večeřová; Petra Holišová; Pallozzi, Emanuele; Guidolotti, Gabriele; Urban, Otmar; Calfapietra, Carlo

    2016-11-01

    Norway spruce (Picea abies) is a strong emitter of biogenic volatile organic compounds (BVOCs). In the present study we investigated how shoot canopy position and high levels of stressors such as high temperature and ozone concentration, affect BVOC emission rates by means of in-situ and ex-situ experimental measurements. Therefore, BVOC emission from current-year spruce shoots was investigated under field and manipulated (temperature, ozone) laboratory conditions. Emitted BVOCs were sampled on desorption tubes, coupled with gas-exchange measurements of CO2 assimilation rate and stomatal conductance, and detected by gas chromatography coupled with mass spectrometry. Total BVOC emission rates from sun shoots under standard conditions were higher than those from shade shoots, although this was significant only in July, on the contrary, only α-pinene and γ-terpinene emission rates showed significant differences between sun and shade acclimated shoots in August. Limonene, α-pinene, β-pinene, and myrcene were identified as the most abundant BVOCs in both campaigns with emission rates above 0.2 nmol m(-2) s(-1). Ex-situ measurements revealed a significantly higher total BVOC emissions under high temperature level (40 °C) by ca. 175% as compared with standard temperature (30 °C), while a short-term fumigation of acute O3 concentration (200 ppb) had no effect on BVOC emissions and its spectrum. These findings might have a relevance considering the role of these compounds in protecting against oxidative stress and their possible stimulation in particular stressful conditions. Implication of such results into emission models may contribute to a more accurate estimation of BVOC emissions for Central European mountain regions dominated by Norway spruce forests and their rate under predicted climate change.

  6. A comparison of genomic selection models across time in interior spruce (Picea engelmannii × glauca) using unordered SNP imputation methods.

    PubMed

    Ratcliffe, B; El-Dien, O G; Klápště, J; Porth, I; Chen, C; Jaquish, B; El-Kassaby, Y A

    2015-12-01

    Genomic selection (GS) potentially offers an unparalleled advantage over traditional pedigree-based selection (TS) methods by reducing the time commitment required to carry out a single cycle of tree improvement. This quality is particularly appealing to tree breeders, where lengthy improvement cycles are the norm. We explored the prospect of implementing GS for interior spruce (Picea engelmannii × glauca) utilizing a genotyped population of 769 trees belonging to 25 open-pollinated families. A series of repeated tree height measurements through ages 3-40 years permitted the testing of GS methods temporally. The genotyping-by-sequencing (GBS) platform was used for single nucleotide polymorphism (SNP) discovery in conjunction with three unordered imputation methods applied to a data set with 60% missing information. Further, three diverse GS models were evaluated based on predictive accuracy (PA), and their marker effects. Moderate levels of PA (0.31-0.55) were observed and were of sufficient capacity to deliver improved selection response over TS. Additionally, PA varied substantially through time accordingly with spatial competition among trees. As expected, temporal PA was well correlated with age-age genetic correlation (r=0.99), and decreased substantially with increasing difference in age between the training and validation populations (0.04-0.47). Moreover, our imputation comparisons indicate that k-nearest neighbor and singular value decomposition yielded a greater number of SNPs and gave higher predictive accuracies than imputing with the mean. Furthermore, the ridge regression (rrBLUP) and BayesCπ (BCπ) models both yielded equal, and better PA than the generalized ridge regression heteroscedastic effect model for the traits evaluated.

  7. Development of high-density SNP genotyping arrays for white spruce (Picea glauca) and transferability to subtropical and nordic congeners.

    PubMed

    Pavy, Nathalie; Gagnon, France; Rigault, Philippe; Blais, Sylvie; Deschênes, Astrid; Boyle, Brian; Pelgas, Betty; Deslauriers, Marie; Clément, Sébastien; Lavigne, Patricia; Lamothe, Manuel; Cooke, Janice E K; Jaramillo-Correa, Juan P; Beaulieu, Jean; Isabel, Nathalie; Mackay, John; Bousquet, Jean

    2013-03-01

    High-density SNP genotyping arrays can be designed for any species given sufficient sequence information of high quality. Two high-density SNP arrays relying on the Infinium iSelect technology (Illumina) were designed for use in the conifer white spruce (Picea glauca). One array contained 7338 segregating SNPs representative of 2814 genes of various molecular functional classes for main uses in genetic association and population genetics studies. The other one contained 9559 segregating SNPs representative of 9543 genes for main uses in population genetics, linkage mapping of the genome and genomic prediction. The SNPs assayed were discovered from various sources of gene resequencing data. SNPs predicted from high-quality sequences derived from genomic DNA reached a genotyping success rate of 64.7%. Nonsingleton in silico SNPs (i.e. a sequence polymorphism present in at least two reads) predicted from expressed sequenced tags obtained with the Roche 454 technology and Illumina GAII analyser resulted in a similar genotyping success rate of 71.6% when the deepest alignment was used and the most favourable SNP probe per gene was selected. A variable proportion of these SNPs was shared by other nordic and subtropical spruce species from North America and Europe. The number of shared SNPs was inversely proportional to phylogenetic divergence and standing genetic variation in the recipient species, but positively related to allele frequency in P. glauca natural populations. These validated SNP resources should open up new avenues for population genetics and comparative genetic mapping at a genomic scale in spruce species.

  8. Impact of eastern dwarf mistletoe (Arceuthobium pusillum) on host white spruce (Picea glauca) development, growth and performance across multiple scales.

    PubMed

    Logan, Barry A; Reblin, Jaret S; Zonana, David M; Dunlavey, Ryan F; Hricko, Carolyn R; Hall, Adam W; Schmiege, Stephanie C; Butschek, Ross A; Duran, Kristy L; Emery, R J Neil; Kurepin, Leonid V; Lewis, James D; Pharis, Richard P; Phillips, Nathan G; Tissue, David T

    2013-04-01

    Infection by eastern dwarf mistletoe (Arceuthobium pusillum) modifies needle and branch morphology and hastens white spruce (Picea glauca) mortality. We examined potential causal mechanisms and assessed the impacts of infection-induced alterations to host development and performance across scales ranging from needle hormone contents to bole expansion. Needles on infected branches (IBs) possessed higher total cytokinin (CK) and lower abscisic acid contents than needles on uninfected branches (UBs). IBs exhibited greater xylem growth than same-aged UBs, which is consistent with the promotive effect of CKs on vascular differentiation and organ sink strength. Elevated CK content may also explain the dense secondary and tertiary branching observed at the site of infection, i.e. the formation of 'witches' brooms' with significantly lower light capture efficiencies. Observed hormone perturbations were consistent with higher rates of transpiration, lower water use efficiencies (WUEs) and more negative needle carbon isotope ratios observed for IBs. Observed reductions in needle size allowed IBs to compensate for reduced hydraulic conductivity. Severe infections resulted in dramatically decreased diameter growth of the bole. It seems likely that the modifications to host hormone contents by eastern dwarf mistletoe infection led white spruce trees to dedicate a disproportionate fraction of their photoassimilate and other resources to self-shaded branches with low WUE. This would have decreased the potential for fixed carbon accumulation, generating a decline in the whole-tree resource pool. As mistletoe infections grew in size and the number of IBs increased, this burden was manifested as increasingly greater reductions in bole growth.

  9. Secondary metabolites of the lichen Hypogymnia physodes (L.) Nyl. and their presence in spruce (Picea abies (L.) H. Karst.) bark.

    PubMed

    Latkowska, Ewa; Bober, Beata; Chrapusta, Ewelina; Adamski, Michal; Kaminski, Ariel; Bialczyk, Jan

    2015-10-01

    Lichen species typically have a characteristic profile of secondary metabolites. Dense populations of Hypogymnia physodes growing frequently as epiphytes on tree branches have harmful effects on the host, likely due to their secondary compounds, which were undetected in tree tissues until now. The aim of the present study was to re-characterise the suite of secondary metabolites of H. physodes thalli and to estimate their translocation into spruce (Picea abies) bark. Thallus and bark extracts were compared using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The compounds were identified based on their UV, MS and MS/MS spectra as well as retention factors of their TLC analysis. In addition to the previously described secondary metabolites (protocetraric, physodalic, 3-hydroxyphysodic, physodic, and 2'-O-methylphysodic acids, atranorin and chloroatranorin) of H. physodes, further three were identified in its thalli: conphysodalic, 4-O-methylphysodic and α-alectoronic acids. Fragmentation patterns from the negative ionisation of each compound were proposed, some of which were described for the first time. Among all of the detected lichen substances, a few, e.g., physodalic, 3-hydroxyphysodic, physodic acids and atranorin, were present in the bark of spruce branches that were abundantly colonised by lichen. The newly identified compounds of H. physodes thalli may belong to its constant or accessory secondary metabolites. These compounds may be useful in the chemotaxonomic classification of this species. The presence of some lichen substances in spruce bark confirmed their ability to penetrate host tissues. These data suggest that H. physodes compounds may cause long-term effects on spruces in nature.

  10. Fine-scale geographic variation in photosynthetic-related traits of Picea glauca seedlings indicates local adaptation to climate.

    PubMed

    Benomar, Lahcen; Lamhamedi, Mohammed S; Villeneuve, Isabelle; Rainville, André; Beaulieu, Jean; Bousquet, Jean; Margolis, Hank A

    2015-08-01

    Climate-related variations in functional traits of boreal tree species can result both from physiological acclimation and genetic adaptation of local populations to their biophysical environment. To improve our understanding and prediction of the physiological and growth responses of populations to climate change, we studied the role of climate of seed origin in determining variations in functional traits and its implications for tree improvement programs for a commonly reforested boreal conifer, white spruce (Picea glauca (Moench) Voss). We evaluated growth, root-to-shoot ratio (R/S), specific leaf area (SLA), needle nitrogen (N(mass)), total non-structural carbohydrates (NSC) and photosynthetic traits of 3-year-old seedlings in a greenhouse experiment using seed from six seed orchards (SO) representing the different regions where white spruce is reforested in Québec. Height and total dry mass (TDM) were positively correlated with photosynthetic capacity (A(max)), stomatal conductance (g(s)) and mesophyll conductance (g(m)). Total dry mass, but not height growth, was strongly correlated with latitude of seed origin (SO) and associated climate variables. A(max), g(s), g(m) and more marginally, photosynthetic nitrogen-use efficiency (PNUE) were positively associated with the mean July temperature of the SO, while water use efficiency (WUE) was negatively associated. Maximum rates of carboxylation (V(cmax)), maximum rates of electron transport (J(max)), SLA, N(mass), NSC and R/S showed no pattern. Our results did not demonstrate a higher Amax for northern seed orchards, although this has been previously hypothesized as an adaptation mechanism for maintaining carbon uptake in northern regions. We suggest that gs, gm, WUE and PNUE are the functional traits most associated with fine-scale geographic clines and with the degree of local adaptation of white spruce populations to their biophysical environments. These geographic patterns may reflect in situ adaptive genetic

  11. A Picea abies Linkage Map Based on SNP Markers Identifies QTLs for Four Aspects of Resistance to Heterobasidion parviporum Infection

    PubMed Central

    Lind, Mårten; Källman, Thomas; Chen, Jun; Ma, Xiao-Fei; Bousquet, Jean; Morgante, Michele; Zaina, Giusi; Karlsson, Bo; Elfstrand, Malin; Lascoux, Martin; Stenlid, Jan

    2014-01-01

    A consensus linkage map of Picea abies, an economically important conifer, was constructed based on the segregation of 686 SNP markers in a F1 progeny population consisting of 247 individuals. The total length of 1889.2 cM covered 96.5% of the estimated genome length and comprised 12 large linkage groups, corresponding to the number of haploid P. abies chromosomes. The sizes of the groups (from 5.9 to 9.9% of the total map length) correlated well with previous estimates of chromosome sizes (from 5.8 to 10.8% of total genome size). Any locus in the genome has a 97% probability to be within 10 cM from a mapped marker, which makes the map suited for QTL mapping. Infecting the progeny trees with the root rot pathogen Heterobasidion parviporum allowed for mapping of four different resistance traits: lesion length at the inoculation site, fungal spread within the sapwood, exclusion of the pathogen from the host after initial infection, and ability to prevent the infection from establishing at all. These four traits were associated with two, four, four and three QTL regions respectively of which none overlapped between the traits. Each QTL explained between 4.6 and 10.1% of the respective traits phenotypic variation. Although the QTL regions contain many more genes than the ones represented by the SNP markers, at least four markers within the confidence intervals originated from genes with known function in conifer defence; a leucoanthocyanidine reductase, which has previously been shown to upregulate during H. parviporum infection, and three intermediates of the lignification process; a hydroxycinnamoyl CoA shikimate/quinate hydroxycinnamoyltransferase, a 4-coumarate CoA ligase, and a R2R3-MYB transcription factor. PMID:25036209

  12. Pinus flexilis and Picea engelmannii share a simple and consistent needle endophyte microbiota with a potential role in nitrogen fixation

    PubMed Central

    Carrell, Alyssa A.; Frank, Anna C.

    2014-01-01

    Conifers predominantly occur on soils or in climates that are suboptimal for plant growth. This is generally attributed to symbioses with mycorrhizal fungi and to conifer adaptations, but recent experiments suggest that aboveground endophytic bacteria in conifers fix nitrogen (N) and affect host shoot tissue growth. Because most bacteria cannot be grown in the laboratory very little is known about conifer–endophyte associations in the wild. Pinus flexilis (limber pine) and Picea engelmannii (Engelmann spruce) growing in a subalpine, nutrient-limited environment are potential candidates for hosting endophytes with roles in N2 fixation and abiotic stress tolerance. We used 16S rRNA pyrosequencing to ask whether these conifers host a core of bacterial species that are consistently associated with conifer individuals and therefore potential mutualists. We found that while overall the endophyte communities clustered according to host species, both conifers were consistently dominated by the same phylotype, which made up 19–53% and 14–39% of the sequences in P. flexilis and P. engelmannii, respectively. This phylotype is related to Gluconacetobacter diazotrophicus and other N2 fixing acetic acid bacterial endophytes. The pattern observed for the P. flexilis and P. engelmannii needle microbiota—a small number of major species that are consistently associated with the host across individuals and species—is unprecedented for an endophyte community, and suggests a specialized beneficial endophyte function. One possibility is endophytic N fixation, which could help explain how conifers can grow in severely nitrogen-limited soil, and why some forest ecosystems accumulate more N than can be accounted for by known nitrogen input pathways. PMID:25071746

  13. A comparison of genomic selection models across time in interior spruce (Picea engelmannii × glauca) using unordered SNP imputation methods

    PubMed Central

    Ratcliffe, B; El-Dien, O G; Klápště, J; Porth, I; Chen, C; Jaquish, B; El-Kassaby, Y A

    2015-01-01

    Genomic selection (GS) potentially offers an unparalleled advantage over traditional pedigree-based selection (TS) methods by reducing the time commitment required to carry out a single cycle of tree improvement. This quality is particularly appealing to tree breeders, where lengthy improvement cycles are the norm. We explored the prospect of implementing GS for interior spruce (Picea engelmannii × glauca) utilizing a genotyped population of 769 trees belonging to 25 open-pollinated families. A series of repeated tree height measurements through ages 3–40 years permitted the testing of GS methods temporally. The genotyping-by-sequencing (GBS) platform was used for single nucleotide polymorphism (SNP) discovery in conjunction with three unordered imputation methods applied to a data set with 60% missing information. Further, three diverse GS models were evaluated based on predictive accuracy (PA), and their marker effects. Moderate levels of PA (0.31–0.55) were observed and were of sufficient capacity to deliver improved selection response over TS. Additionally, PA varied substantially through time accordingly with spatial competition among trees. As expected, temporal PA was well correlated with age-age genetic correlation (r=0.99), and decreased substantially with increasing difference in age between the training and validation populations (0.04–0.47). Moreover, our imputation comparisons indicate that k-nearest neighbor and singular value decomposition yielded a greater number of SNPs and gave higher predictive accuracies than imputing with the mean. Furthermore, the ridge regression (rrBLUP) and BayesCπ (BCπ) models both yielded equal, and better PA than the generalized ridge regression heteroscedastic effect model for the traits evaluated. PMID:26126540

  14. Imaging of Dynamic Secretory Vesicles in Living Pollen Tubes of Picea meyeri Using Evanescent Wave Microscopy1[W

    PubMed Central

    Wang, Xiaohua; Teng, Yan; Wang, Qinli; Li, Xiaojuan; Sheng, Xianyong; Zheng, Maozhong; Šamaj, Jozef; Baluška, František; Lin, Jinxing

    2006-01-01

    Evanescent wave excitation was used to visualize individual, FM4-64-labeled secretory vesicles in an optical slice proximal to the plasma membrane of Picea meyeri pollen tubes. A standard upright microscope was modified to accommodate the optics used to direct a laser beam at a variable angle. Under evanescent wave microscopy or total internal reflection fluorescence microscopy, fluorophores localized near the surface were excited with evanescent waves, which decay exponentially with distance from the interface. Evanescent waves with penetration depths of 60 to 400 nm were generated by varying the angle of incidence of the laser beam. Kinetic analysis of vesicle trafficking was made through an approximately 300-nm optical section beneath the plasma membrane using time-lapse evanescent wave imaging of individual fluorescently labeled vesicles. Two-dimensional trajectories of individual vesicles were obtained from the resulting time-resolved image stacks and were used to characterize the vesicles in terms of their average fluorescence and mobility, expressed here as the two-dimensional diffusion coefficient D2. The velocity and direction of vesicle motions, frame-to-frame displacement, and vesicle trajectories were also calculated. Analysis of individual vesicles revealed for the first time, to our knowledge, that two types of motion are present, and that vesicles in living pollen tubes exhibit complicated behaviors and oscillations that differ from the simple Brownian motion reported in previous investigations. Furthermore, disruption of the actin cytoskeleton had a much more pronounced effect on vesicle mobility than did disruption of the microtubules, suggesting that actin cytoskeleton plays a primary role in vesicle mobility. PMID:16798949

  15. The mycorrhizal community in a forest chronosequence of Sitka spruce [Picea sitchensis (Bong.) Carr.] in Northern England.

    PubMed

    Palfner, Götz; Casanova-Katny, M Angélica; Read, David J

    2005-11-01

    Demography and fungal diversity of the belowground ectomycorrhizal community in a chronosequence of Sitka spruce [Picea sitchensis (Bong.) Carr.] in Northumberland, Northern England, were analysed; mycorrhizal root samples were taken from 6-, 12-, 30- and 40-year-old stands, and fungal fruiting bodies were collected in autumn to complement the survey. Naturally germinated seedlings less than 1 year of age (taken from the 30-year-old stand) were also examined. A total of 118,000 mycorrhizal root tips were extracted from 40 soil cores (ten per age class) and from the complete root systems of 25 seedlings and separated into active and senescent root tips according to their morphology and anatomy. Active tips were distinguished according to their mycobionts which were characterised and identified microscopically. Although almost 100% of all fine roots were mycorrhizal, EM fungal diversity throughout the chronosequence was low, consisting of a total of 16 species of which three were only found as fruiting bodies. Of the six mycobionts found most regularly below ground, Tylospora fibrillosa was the most common, colonising about 70% of all root tips and more than 90% of those of seedlings and young trees. Root density and mycorrhizal diversity increased, but percentage of vital root tips decreased with increasing tree age, levelling off in the 30- and 40-year-old stand. Among the five subdominant fungal species, Dermocybe crocea was found to have its peak of distribution in the 12-year-old stand and Russula emetica, Lactarius rufus, Hymenoscyphus ericae agg. and the unidentified Piceirhiza sulfo-incrustata in the 30- and 40-year-old stands. The possible correlations between the mycorrhizal community structure and biotic and abiotic factors are discussed.

  16. Geological characteristics of the Shinkai Seep Field, a serpentinite-hosted ecosystem in the Southern Mariana Forearc

    NASA Astrophysics Data System (ADS)

    Ohara, Y.; Stern, R. J.; Martinez, F.; Michibayashi, K.; Reagan, M. K.; Fujikura, K.; Watanabe, H.; Ishii, T.; Kelley, K. A.

    2012-12-01

    Most hydrothermal vents along mid-ocean spreading ridges are high-temperature, sulfide-rich, and low pH (acidic environments). For this reason, the discovery of the Lost City hydrothermal field on the Mid-Atlantic Ridge has stimulated interest in the role of serpentinization of peridotite in generating H2- and CH4-rich fluids and associated carbonate chimneys, as well as in the biological communities adapted to highly reduced, alkaline environments. A new serpentinite-hosted ecosystem, the Shinkai Seep Field (SSF), was discovered by a Shinkai 6500 dive in the inner trench slope of the southern Mariana Trench, near the Challenger Deep, during YK10-12 cruise of R/V Yokosuka in September 2010. Abundant chemosynthetic biological communities, principally consisting of vesicomyid clams are associated with serpentinized peridotite in the SSF. Serpentinization beneath several hydrothermal sites on the Mid-Atlantic Ridge is controlled by interacting seawater and peridotite, variably influenced by magmatic heat. In contrast, the SSF is located in a deep inner trench slope where magmatic heat contribution is unlikely. Instead, serpentinization reactions feeding the SSF may be controlled by persistent fluid flow from the subducting slab. Slab-derived fluid flow is probably controlled by flow through fractures because no serpentinite mud volcano can be discerned along the southern Mariana forearc. Deep-towed IMI-30 sonar backscatter imaging during TN273 cruise of R/V Thomas G. Thompson in January 2012 indicates that the SSF is associated with a small, low backscatter feature that may be a small mound. There are 20 or more of these features in the imaged area, the size of which is ~200 m width and ~200 m to ~700 m long. Since the southern Mariana forearc is heavily faulted, with a deep geology that is dominated by peridotite, more SSF-type seeps are likely to exist along the forearc above the Challenger Deep. The discovery of the SSF suggests that serpentinite-hosted vents may

  17. Coral Reef Disturbance and Recovery Dynamics Differ across Gradients of Localized Stressors in the Mariana Islands

    PubMed Central

    Houk, Peter; Benavente, David; Iguel, John; Johnson, Steven; Okano, Ryan

    2014-01-01

    The individual contribution of natural disturbances, localized stressors, and environmental regimes upon longer-term reef dynamics remains poorly resolved for many locales despite its significance for management. This study examined coral reefs in the Commonwealth of the Northern Mariana Islands across a 12-year period that included elevated Crown-of-Thorns Starfish densities (COTS) and tropical storms that were drivers of spatially-inconsistent disturbance and recovery patterns. At the island scale, disturbance impacts were highest on Saipan with reduced fish sizes, grazing urchins, and water quality, despite having a more favorable geological foundation for coral growth compared with Rota. However, individual drivers of reef dynamics were better quantified through site-level investigations that built upon island generalizations. While COTS densities were the strongest predictors of coral decline as expected, interactive terms that included wave exposure and size of the overall fish assemblages improved models (R2 and AIC values). Both wave exposure and fish size diminished disturbance impacts and had negative associations with COTS. However, contrasting findings emerged when examining net ecological change across the 12-year period. Wave exposure had a ubiquitous, positive influence upon the net change in favorable benthic substrates (i.e. corals and other heavily calcifying substrates, R2 = 0.17 for all reeftypes grouped), yet including interactive terms for herbivore size and grazing urchin densities, as well as stratifying by major reeftypes, substantially improved models (R2 = 0.21 to 0.89, lower AIC scores). Net changes in coral assemblages (i.e., coral ordination scores) were more sensitive to herbivore size or the water quality proxy acting independently (R2 = 0.28 to 0.44). We conclude that COTS densities were the strongest drivers of coral decline, however, net ecological change was most influenced by localized stressors, especially herbivore

  18. Geology, Streamflow, and Water Chemistry of the Talufofo Stream Basin, Saipan, Northern Mariana Islands

    USGS Publications Warehouse

    Izuka, Scot K.; Ewart, Charles J.

    1995-01-01

    A study of the geology, streamflow, and water chemistry of Talufofo Stream Basin, Saipan, Commonwealth of the Northern Mariana Islands, was undertaken to determine the flow characteristics of Talufofo Stream and the relation to the geology of the drainage basin. The Commonwealth government is exploring the feasibility of using water from Talufofo Stream to supplement Saipan's stressed municipal water supply. Streamflow records from gaging stations on the principal forks of Talufofo Stream indicate that peak streamflows and long-term average flow are higher at the South Fork gaging station than at the Middle Fork gaging station because the drainage area of the South Fork gaging station is larger, but persistent base flow from ground-water discharge during dry weather is greater in the Middle Fork gaging station. The sum of the average flows at the Middle Fork and South Fork gaging stations, plus an estimate of the average flow at a point in the lower reaches of the North Fork, is about 2.96 cubic feet per second or 1.91 million gallons per day. Although this average represents the theoretical maximum long-term draft rate possible from the Talufofo Stream Basin if an adequate reservoir can be built, the actual amount of surface water available will be less because of evaporation, leaks, induced infiltration, and reservoir-design constraints. Base-flow characteristics, such as stream seepage and spring discharge, are related to geology of the basin. Base flow in the Talufofo Stream Basin originates as discharge from springs near the base of limestones located in the headwaters of Talufofo Stream, flows over low-permeability volcanic rocks in the middle reaches, and seeps back into the high-permeability limestones in the lower reaches. Water sampled from Talufofo Stream during base flow had high dissolved-calcium concentrations (between 35 and 98 milligrams per liter), characteristic of water from a limestone aquifer. Concentrations of potassium, sodium, and chloride

  19. Near-seafloor magnetic field observations at the Mariana Trough back-arc spreading center

    NASA Astrophysics Data System (ADS)

    Fujiwara, Toshiya; Asada, Miho; Umino, Susumu; Koike, Yuki; Kanamatsu, Toshiya

    2010-05-01

    We surveyed the Mariana Trough back-arc basin in the western Pacific with the Japanese submersible Shinkai 6500 to understand detailed crustal formation process at the 17° N segment [Fujiwara et al., 2008]. The 17° N segment is suggested to be in vigorous magmatic stage. Sheet lava flows, suggesting a high rate of eruption, occupy the seafloor of the segment even the slow spreading with a full-rate of ~3 cm/yr [Deschamps et al., 2005; Asada et al., 2007]. The objective of magnetic field measurements is to investigate magnetization of lava flows at the seafloor. Near-seafloor observations provide us high-resolution magnetic anomaly that is valuable for the studies of the detailed magnetization structure of ocean crust and paleointensity recorded in the ocean crust. Magnetization intensities relate to age of lava, therefore deep-sea magnetic data may provide geophysical evidence for discussion of relative age differences of the lava flows. Three submersible dives were made in the axial valley situated in the spreading center. One of the dives traversed the axial valley a distance of ~2 km from the center of the valley toward off-axis, roughly parallel to the spreading direction. We observed magnetic anomalies with large-amplitude (up to 5000 nT) and short-wavelength (several tens of meters). We evaluated fine-scale across-axis magnetic structure along the dive path from the anomalies. High magnetization intensity (up to 50 A/m) was estimated at the center of the axial valley, and therefore the lava flows in the area was likely young in age. The magnetization intensity decreased toward the off-axis. The result suggests the seafloor age increases toward the off-axis. However the detailed variation of the magnetization distribution does not show simple seafloor age increment in proportion to distance from the spreading center. It implies the complexity of the crustal formation process. There is no clear correlation between the distribution of magnetization intensity

  20. Tilt Recorded by a Portable Broadband Seismograph: The 2003 eruption of Anatahan, Northern Mariana Islands

    NASA Astrophysics Data System (ADS)

    Shore, P. J.; Wiens, D. A.; Pozgay, S.; Sauter, A.; White, R.

    2004-12-01

    The horizontal components of broadband seismographs are highly sensitive to tilt, suggesting that widely deployed portable broadband seismic sensors may record important tilt information associated with volcanic eruptions. We report on a tilt episode that coincides with the first historical eruption of Anatahan volcano on May 10, 2003. The tilt was recorded by a portable PASSCAL STS-2 seismograph fortuitously deployed four days prior to the eruption as part of the Mariana Subduction Factory Imaging Experiment. The seismograph, located in an underground insulated chamber about 6 km west of the active vent, recorded continuously throughout the eruption sequence. A long-period signal with a dominant period of several hours was recorded on the EW component beginning at 06:30 GMT on May 10, which coincides with the onset of continuous volcano-tectonic (VT) seismicity and is one hour prior to the eruption time estimated by the Volcanic Ash Advisory Center based on satellite photos. The signal was much larger than a long period diurnal signal presumably resulting from temperature perturbations. A much smaller signal was recorded on the NS component, and the signal was totally absent on the vertical component, suggesting it results from tilt that is radial with respect to the active vent. An estimate of the tilt as a function of time was recovered by deconvolving to acceleration within a passband of 500-50,000 seconds, and dividing by g. The tilt signal records an initial episode of tilt down away from the volcanic center from 06:30 - 09:30 GMT, which we interpret as inflation of the shallow volcanic source. The tilt reverses and records deflation from 09:30 until 17:50, after which any large tilt signal ceases. The period of inflation corresponds to a period of numerous VT events, whereas fewer events were recorded during the deflation episode, and the VT events resumed again after the end of the deflationary tilt. The maximum tilt is about 2 microradians, although it is

  1. Seismic investigations of the Mariana subduction system: Anisotropy, attenuation, and volcano seismology

    NASA Astrophysics Data System (ADS)

    Pozgay, Sara Harriet

    With an unprecedented dataset for studying questions regarding subduction dynamics and backarc spreading, I describe two seismic investigations of the structure and dynamics of the Mariana subduction system (seismic anisotropy and seismic attenuation) and an investigation of the seismic characteristics of an unexpected volcanic eruption. Results include: (1) The fortuitous timing of the Anatahan Island seismograph installation recorded most of the May 2003 eruption of the historically inactive volcano. Temporal and spatial analyses of three different types of volcano-related earthquakes, volcanic tremor, and a tilt signal together provide an accurate picture of the seismotectonics related to the eruption. We observe very little precursory seismic activity until ˜6 hours before the estimated eruption onset. A prominent tilt signal, indicative of inflation and deflation of the magma chamber, is coincident with the onset of nearly continuous earthquake activity ˜1 hour before the estimated eruption time. (2) Tomographic inversion of ˜2800 path-averaged seismic attenuation t* measurements for 2-D QP-1 and QP/QS structures and subsequent calculation of QS structure illuminate a low attenuation slab and high attenuation regions beneath the arc and the backarc spreading center and in the core of the mantle wedge. A quasi-columnar-shaped high QP-1 region persists beneath the backarc spreading center to ˜100-125 km depth and is distinctly separate at shallow depths (< ˜50 km) from the high attenuation island arc anomaly. In addition, we observe high Q P-1 and QS-1 beneath forearc seamounts, likely indicative of serpentinization. (3) Results from ˜300 shear wave splitting measurements show dominantly arc-parallel fast directions near the island arc and between the arc and the trough. Only from deep (>250 km) earthquakes and at stations located in the far backarc are fast splitting directions concurrent with a corner flow regime. These results suggest that the typical

  2. Iron Stable Isotopes, Magmatic Differentiation and the Oxidation State of Mariana Arc Magmas

    NASA Astrophysics Data System (ADS)

    Williams, H. M.; Prytulak, J.; Plank, T. A.; Kelley, K. A.

    2014-12-01

    Arc magmas are widely considered to be oxidized, with elevated ferric iron contents (Fe3+/ΣFe) relative to mid-ocean ridge lavas (1, 2). However, it is unclear whether the oxidized nature of arc basalts is a primary feature, inherited from the sub-arc mantle, or the product of magmatic differentiation and/or post eruptive alteration processes (3). Iron stable isotopes can be used to trace the distribution of Fe during melting and magmatic differentiation processes (4, 5). Here we present Fe isotope data for well-characterized samples (6-8) from islands of the Central Volcanic Zone (CVZ) of the intra-oceanic Mariana Arc to explore the effect of magmatic differentiation processes on Fe isotope systematics. The overall variation in the Fe isotope compositions (δ57Fe) of samples from the CVZ islands ranges from -0.10 ±0.04 to 0.29 ± 0.01 ‰. Lavas from Anatahan are displaced to lower overall δ57Fe values (range -0.10 ±0.04 to 0.18 ±0.01 ‰) relative to other CVZ samples. Fe isotopes in the Anatahan suite (range -0.10 ±0.04 to 0.18 ±0.01 ‰) are positively correlated with SiO2 and negatively correlated with Ca, Fe2O3(t), Cr and V and are displaced to lower overall δ57Fe values relative to other CVZ samples. These correlations can be interpreted in terms of clinopyroxene and magnetite fractionation, with magnetite saturation throughout the differentiation sequence. Magnetite saturation is further supported by negative correlations between V, Fe2O3(t), Cr and MgO (for MgO <3.5 wt%). The early saturation of magnetite in the Anatahan and CVZ lavas is likely to be a function of high melt water content (9, 10) and potentially elevated melt oxidation state. Future work will focus on determining the relationships between mineral Fe isotope partitioning effects and melt composition and oxidation state. 1. R. Arculus, Lithos (1994). 2. K. A. Kelley et al., Science (2009). 3. C.-T. A. Lee et al., J. Pet. (2005). 4. N. Dauphas et al., EPSL (2014). 5. P. A. Sossi et al

  3. Geological Characteristics of the 2003 Eruption at Anatahan, Northern Mariana Islands

    NASA Astrophysics Data System (ADS)

    Nakada, S.; Matsushima, T.; Kato, T.; Camacho, J. T.; Chong, R.

    2003-12-01

    During GPS campaign of the Northern Marianas, we carried out geological inspection about the May-June eruptions at Anatahan, landing on 8 points within the island in mid-July. The active crater, located at the bottom of the eastern caldera, has the dimension of about 300 m across and 100 m deep, and wreck of a lava dome, whose appearance was witnessed first in early June, was observed in the northern periphery. The highest temperature measured within the crater was as high as about 300 C, the crater bottom was filled with mud poured into from the surrounding steep walls. Tephra of the eruptions is thicker in the western part: up to about 1 m thick in the western caldera. Small rills and gullies grow extensively over the deposits. Total volume of eruption products can be roughly estimated at a few tens of million m3. Deposits are composed basically of lower brown and upper gray tephra layers. Both consisting of pyroclastic fall and surge deposits. Brown tephra layer contains various colored vesicular pumice, and larger and denser pumice is accumulated in the uppermost part. Gray tephra is very fine ashes, even on crater rim, deposited by falling including a lateral movement from the crater side. Though some of them were burnt in surge deposits near the crater, vegetation had stood during deposition of brown tephra and was toppled down by deposition of gray tephra. A block probably of broken lava dome is covered only by gray tephra. All pumices, block of lava dome, and masses of brown and gray tephra are andesitic in composition (60-61 % SiO2). Following the eruptive activity of brown tephra since mid-May, another explosive event of phreatomagmatic eruptions occurred probably in mid June, the latter was just preceded by breaking of newly-formed lava dome. As the active crater probably was deepened with time during eruptions, magma reacted with water invaded from aquifer or sea, resulting in the explosive eruptions. Only finer particles of quenched and fragmented

  4. Izu-Bonin-Mariana forearc crust as a modern ophiolite analogue

    NASA Astrophysics Data System (ADS)

    Ishizuka, Osamu; Tani, Kenichiro; Reagan, Mark; Kanayama, Kyoko; Umino, Susumu; Harigane, Yumiko

    2013-04-01

    Recent geological and geophysical surveys in the Izu-Bonin-Mariana (IBM) fore-arc have revealed the occurrence on the seafloor of oceanic crust generated in the initial stages of subduction and embryonic island arc formation. The observed forearc section is composed of (from bottom to top): (1) mantle peridotite, (2) gabbroic rocks, (3) a sheeted dyke complex, (4) basaltic pillow lavas, (5) boninites and magnesian andesites, and (6) tholeiites and calc-alkaline arc lavas. The oldest magmatism after subduction initiation generated forearc basalts (FAB) between 52 and 48 Ma, and then boninitic and calc-alkaline lavas that collectively make up the extrusive sequence of the forearc oceanic crust. The change from FAB magmatism to flux melting and boninitic volcanism took 2-4 m.y., and the change to flux melting in counter-flowing mantle and "normal" arc magmatism took 7-8 m.y. This evolution from subduction initiation to true subduction occurred nearly simultaneously along the entire length of the IBM subduction system. One important characteristic feature of the common forearc stratigraphy in the IBM forearc is the association of sheeted dykes with basaltic pillow lavas, which strongly implies that the eruption of FAB was associated with seafloor spreading. This is supported by the seismic velocity structure of the Bonin Ridge area (Kodaira et al., 2010), showing it to have a thin ocean-ridge-like crust (< 10km). It appears that the FAB was produced by sea-floor spreading associated with subduction initiation along the length of the IBM forearc. A potential location of subduction nucleation along the Mesozoic-aged crust has been found along the margins of the West Philippine Basin. One possible scenario for subduction initiation at the IBM arc was that it was induced by overthrusting of the Mesozoic arc and backarc or forearc terranes bounding the east side of the Asian Plate over the Pacific Plate, followed by failure of the Pacific plate lithosphere and subduction

  5. Hydrothermal circulation system in the central Mariana illustrated by Magnetometoric Resistivity experiments

    NASA Astrophysics Data System (ADS)

    Tada, N.; Seama, N.; Goto, T.; Kido, M.

    2004-12-01

    Hydrothermal vent fields are known to exist on the spreading axis, where sea water penetrates into the crust and upwells through the hydrothermal vents. Understanding of the hydrothermal circulation system is extremely important to reveal the cooling process of the oceanic crust. The thermal structure beneath the hydrothermal vent reflects the extent of underground activity and the convection scale of the hot water. Temperature in the crust can be estimated from the electrical conductivity because the conductivity depends on the water volume, the salinity concentration and the temperature of the sea water in the crust. The Alice Spring Field (18 o12.9'N, 144 o42.5'E and 3600m deep), on the spreading axis in the central Mariana Back-Arc Basin, is a suitable site for this purpose. Hydrothermal vent in this field was firstly discovered by Alvin in 1987 (Hawkins et al., 1990). Shinkai6500 also confirmed the hydrothermal activity in 1992 and 1996 (Gamou et al., 1994; Fujikura et al., 1997). In November, 2002, we conducted Magnetometric Resistivity (MMR) survey using R/V Kairei, JAMSTEC in this field. In the MMR method, controlled electric current was applied from a pair of electrodes; one is just beneath the sea surface and the other is close to the seafloor. To record electoromagnetic responses of the crust to the inputed current, we deployed six ocean bottom electromagnetometers (OBEMs), which can measure 3-components of magnetic and electric fields simultaneously. Measurements were conducted at 34 sites around the field, each of which consists of 30 minutes stacking for repeated current signals to keep better S/N ratio. Apparent resistivity is given by a function of amplitudes of magnetic field variation and source-receiver distance. We recovered the data from four OBEMs (two were on the spreading axis and other two were off axis). The plot of magnetic amplitudes to source-receiver distances shows different trend between OBEMs on-axis and off-axis. Therefore, we

  6. Coral reef disturbance and recovery dynamics differ across gradients of localized stressors in the Mariana Islands.

    PubMed

    Houk, Peter; Benavente, David; Iguel, John; Johnson, Steven; Okano, Ryan

    2014-01-01

    The individual contribution of natural disturbances, localized stressors, and environmental regimes upon longer-term reef dynamics remains poorly resolved for many locales despite its significance for management. This study examined coral reefs in the Commonwealth of the Northern Mariana Islands across a 12-year period that included elevated Crown-of-Thorns Starfish densities (COTS) and tropical storms that were drivers of spatially-inconsistent disturbance and recovery patterns. At the island scale, disturbance impacts were highest on Saipan with reduced fish sizes, grazing urchins, and water quality, despite having a more favorable geological foundation for coral growth compared with Rota. However, individual drivers of reef dynamics were better quantified through site-level investigations that built upon island generalizations. While COTS densities were the strongest predictors of coral decline as expected, interactive terms that included wave exposure and size of the overall fish assemblages improved models (R2 and AIC values). Both wave exposure and fish size diminished disturbance impacts and had negative associations with COTS. However, contrasting findings emerged when examining net ecological change across the 12-year period. Wave exposure had a ubiquitous, positive influence upon the net change in favorable benthic substrates (i.e. corals and other heavily calcifying substrates, R2 = 0.17 for all reeftypes grouped), yet including interactive terms for herbivore size and grazing urchin densities, as well as stratifying by major reeftypes, substantially improved models (R2 = 0.21 to 0.89, lower AIC scores). Net changes in coral assemblages (i.e., coral ordination scores) were more sensitive to herbivore size or the water quality proxy acting independently (R2 = 0.28 to 0.44). We conclude that COTS densities were the strongest drivers of coral decline, however, net ecological change was most influenced by localized stressors, especially herbivore sizes and

  7. Coral reef disturbance and recovery dynamics differ across gradients of localized stressors in the Mariana Islands.

    PubMed

    Houk, Peter; Benavente, David; Iguel, John; Johnson, Steven; Okano, Ryan

    2014-01-01

    The individual contribution of natural disturbances, localized stressors, and environmental regimes upon longer-term reef dynamics remains poorly resolved for many locales despite its significance for management. This study examined coral reefs in the Commonwealth of the Northern Mariana Islands across a 12-year period that included elevated Crown-of-Thorns Starfish densities (COTS) and tropical storms that were drivers of spatially-inconsistent disturbance and recovery patterns. At the island scale, disturbance impacts were highest on Saipan with reduced fish sizes, grazing urchins, and water quality, despite having a more favorable geological foundation for coral growth compared with Rota. However, individual drivers of reef dynamics were better quantified through site-level investigations that built upon island generalizations. While COTS densities were the strongest predictors of coral decline as expected, interactive terms that included wave exposure and size of the overall fish assemblages improved models (R2 and AIC values). Both wave exposure and fish size diminished disturbance impacts and had negative associations with COTS. However, contrasting findings emerged when examining net ecological change across the 12-year period. Wave exposure had a ubiquitous, positive influence upon the net change in favorable benthic substrates (i.e. corals and other heavily calcifying substrates, R2 = 0.17 for all reeftypes grouped), yet including interactive terms for herbivore size and grazing urchin densities, as well as stratifying by major reeftypes, substantially improved models (R2 = 0.21 to 0.89, lower AIC scores). Net changes in coral assemblages (i.e., coral ordination scores) were more sensitive to herbivore size or the water quality proxy acting independently (R2 = 0.28 to 0.44). We conclude that COTS densities were the strongest drivers of coral decline, however, net ecological change was most influenced by localized stressors, especially herbivore

  8. Submersible- and lander-observed community patterns in the Mariana and New Britain trenches: Influence of productivity and depth on epibenthic and scavenging communities

    NASA Astrophysics Data System (ADS)

    Gallo, Natalya D.; Cameron, James; Hardy, Kevin; Fryer, Patricia; Bartlett, Douglas H.; Levin, Lisa A.

    2015-05-01

    Deep-sea trenches remain one of the least explored ocean ecosystems due to the unique challenges of sampling at great depths. Five submersible dives conducted using the DEEPSEA CHALLENGER submersible generated video of undisturbed deep-sea communities at bathyal (994 m), abyssal (3755 m), and hadal (8228 m) depths in the New Britain Trench, bathyal depths near the Ulithi atoll (1192 m), and hadal depths in the Mariana Trench Challenger Deep (10908 m). The New Britain Trench is overlain by waters with higher net primary productivity (~3-fold) than the Mariana Trench and nearby Ulithi, and receives substantially more allochthonous input from terrestrial sources, based on the presence of terrestrial debris in submersible video footage. Comparisons between trenches addressed how differences in productivity regime influence benthic and demersal deep-sea community structure. In addition, the scavenger community was studied using paired lander deployments to the New Britain (8233 m) and Mariana (10918 m) trenches. Differences in allochthonous input were reflected in epibenthic community abundance, biodiversity, and lifestyle representation. More productive locations were characterized by higher faunal abundances (~2-fold) at both bathyal and hadal depths. In contrast, biodiversity trends showed a unimodal pattern with more food-rich areas exhibiting reduced bathyal diversity and elevated hadal diversity. Hadal scavenging communities exhibited similar higher abundance but also ~3-fold higher species richness in the more food-rich New Britain Trench compared to the Mariana Trench. High species- and phylum-level diversity observed in the New Britain Trench suggest that trench environments may foster higher megafaunal biodiversity than surrounding abyssal depths if food is not limiting. However, the absence of fish at our hadal sites suggests that certain groups do have physiological depth limits. Submersible video footage allowed novel in situ observation of holothurian

  9. Water content and bark thickness of Norway spruce (Picea abies) stems: phloem water capacitance and xylem sap flow.

    PubMed

    Gall, Rolf; Landolt, W; Schleppi, P; Michellod, V; Bucher, J B

    2002-06-01

    To determine the relationship between phloem transport and changes in phloem water content, we measured temporal and spatial variations in water content and sucrose, glucose and fructose concentrations in phloem samples and phloem exudates of 70- and 30-year-old Norway spruce trees (Picea abies (L.) Karst.). Large temporal and spatial variations in phloem water content (1.4-2.6 mg mg(dw)(-1)) and phloem total sugar concentration (31-70 mg g(dw)(-1)) paralleled each other (r(2) = 0.83, P < 0.0001 for the temporal profile and r(2) = 0.96, P < 0.008 for the spatial profile), indicating that phloem water content depends on the total amount of sugar to be transferred. Changes in phloem water content were unrelated to changes in bark thickness. Maximum changes in phloem water content calculated from dendrometer readings were only 8-11% of the maximum measured changes in phloem water content, indicating that reversible changes in bark thickness did not reflect changes in internal water relations. We also studied the relationship between xylem sap velocity and changes in bark thickness in 70-year-old trees during summer 1999 and winter 1999-2000. Sap flow occurred sporadically throughout the winter, but there was no relationship between bark shrinkage or swelling and sap velocity. In winter, mean daily xylem sap velocity was significantly correlated with mean daily vapor pressure deficit and air temperature (P < 0.0001, in both cases). Changes in bark thickness corresponded with both short- and long-term changes in relative humidity, in both winter and summer. Under controlled conditions at > 0 degrees C, changes in relative humidity alone caused changes in thickness of boiled bark samples. Because living bark of Norway spruce trees contains large areas with crushed and dead sieve cell zones-up to 24% of the bark is air-filled space-we suggest that this space can compensate for volume changes in living phloem cells independently of total tissue water content. We conclude

  10. [Response of Picea purpurea and Abies faxoniana tree rings at different slope aspects to rapid warming in western Sichuan, China].

    PubMed

    Guo, Bin-de; Zhang, Yuan-dong; Wang, Xiao-chun

    2016-02-01

    By using an empirical 'signal-free' standardization approach, we constructed four Picea purpurea and Abies faxoniana tree-ring chronologies at southeast and northwest slope aspects of Erdaohai and east slope aspect of Zharisi, Songpan, west Sichuan, China. The response analysis and multivariate analysis of variance between tree rings and climatic variables were conducted to explore the divergent responses of tree growth at different slope aspects to the recent warming climate. Results showed that tree growth of P. purpurea at east slope aspect was obviously accelerated (0.011 a-1) since rapid warming in 1980, whereas those at northwest slope aspect was significantly reduced (-0.006 a-1). Tree growth of P. purpurea at southeast slope aspect and A. faxoniana at northwest slope aspect decreased in significantly. With the rapid warming, growth-climate relationships of P. purpurea and A. faxoniana at different slope aspects changed significantly. After rapid warming in 1980, the promoting effects of growing season temperature (GST) on P. purpurea growth at east slope increased significantly, while the inhibitory effects of GST on its growth at southeast and northwest slopes also increased significantly. However, the effects of GST on A. faxoniana growth at northwest slope did not change significantly before and after rapid warming. The effects of precipitation in May (PM) on P. purpurea growth at east slope was changed from inhibition before rapid warming to significant promotion after rapid warming, while the inhibitory effects of PM on P. purpurea growth at southeast and northwest slopes increased significantly. For A. faioniana at northwest slope, however, it did not change obviously before and after rapid warming. The response analysis between tree growth and the Palmer drought severity index (PDSI) showed that soil moisture variations at different slope aspects were an important reason of tree-ring growth response difference since rapid warming. In addition, the

  11. Evolutionary history of Purple cone spruce (Picea purpurea) in the Qinghai-Tibet Plateau: homoploid hybrid origin and Pleistocene expansion.

    PubMed

    Sun, Yongshuai; Abbott, Richard J; Li, Lili; Li, Long; Zou, Jiabin; Liu, Jianquan

    2014-02-01

    Hybridization and introgression can play an important role in speciation. Here, we examine their roles in the origin and evolution of Picea purpurea, a diploid spruce species occurring on the Qinghai-Tibet Plateau (QTP). Phylogenetic relationships and ecological differences between this species and its relatives, P. schrenkiana, P. likiangensis and P. wilsonii, are unclear. To clarify them, we surveyed sequence variation within and between them for 11 nuclear loci, three chloroplast (cp) and two mitochondrial (mt) DNA fragments, and examined their ecological requirements using ecological niche modelling. Initial analyses based on 11 nuclear loci rejected a close relationship between P. schrenkiana and P. purpurea. BP&P tests and ecological niche modelling indicated substantial divergence between the remaining three species and supported the species status of P. purpurea, which contained many private alleles as expected for a well-established species. Sequence variation for cpDNA and mtDNA suggested a close relationship between P. purpurea and P. wilsonii, while variation at the nuclear se1364 gene suggested P. purpurea was more closely related to P. likiangensis. Analyses of genetic divergence, Bayesian clustering and model comparison using approximate Bayesian computation (ABC) of nuclear (nr) DNA variation all supported the hypothesis that P. purpurea originated by homoploid hybrid speciation from P. wilsonii and P. likiangensis. The ABC analysis dated the origin of P. purpurea at the Pleistocene, and the estimated hybrid parameter indicated that 69% of its nuclear composition was contributed by P. likiangensis and 31% by P. wilsonii. Our results further suggested that during or immediately following its formation, P. purpurea was subject to organelle DNA introgression from P. wilsonii such that it came to possess both mtDNA and cpDNA of P. wilsonii. The estimated parameters indicated that following its origin, P. purpurea underwent an

  12. Population dynamics and genetic changes of Picea abies in the South Carpathians revealed by pollen and ancient DNA analyses

    PubMed Central

    2011-01-01

    Background Studies on allele length polymorphism designate several glacial refugia for Norway spruce (Picea abies) in the South Carpathian Mountains, but infer only limited expansion from these refugia after the last glaciation. To better understand the genetic dynamics of a South Carpathian spruce lineage, we compared ancient DNA from 10,700 and 11,000-year-old spruce pollen and macrofossils retrieved from Holocene lake sediment in the Retezat Mountains with DNA extracted from extant material from the same site. We used eight primer pairs that amplified short and variable regions of the spruce cpDNA. In addition, from the same lake sediment we obtained a 15,000-years-long pollen accumulation rate (PAR) record for spruce that helped us to infer changes in population size at this site. Results We obtained successful amplifications for Norway spruce from 17 out of 462 pollen grains tested, while the macrofossil material provided 22 DNA sequences. Two fossil sequences were found to be unique to the ancient material. Population genetic statistics showed higher genetic diversity in the ancient individuals compared to the extant ones. Similarly, statistically significant Ks and Kst values showed a considerable level of differentiation between extant and ancient populations at the same loci. Lateglacial and Holocene PAR values suggested that population size of the ancient population was small, in the range of 1/10 or 1/5 of the extant population. PAR analysis also detected two periods of rapid population growths (from ca. 11,100 and 3900 calibrated years before present (cal yr BP)) and three bottlenecks (around 9180, 7200 and 2200 cal yr BP), likely triggered by climatic change and human impact. Conclusion Our results suggest that the paternal lineages observed today in the Retezat Mountains persisted at this site at least since the early Holocene. Combination of the results from the genetic and the PAR analyses furthermore suggests that the higher level of genetic

  13. Genetic Adaptation vs. Ecophysiological Plasticity of Photosynthetic-Related Traits in Young Picea glauca Trees along a Regional Climatic Gradient.

    PubMed

    Benomar, Lahcen; Lamhamedi, Mohammed S; Rainville, André; Beaulieu, Jean; Bousquet, Jean; Margolis, Hank A

    2016-01-01

    Assisted population migration (APM) is the intentional movement of populations within a species range to sites where future environmental conditions are projected to be more conducive to growth. APM has been proposed as a proactive adaptation strategy to maintain forest productivity and to reduce the vulnerability of forest ecosystems to projected climate change. The validity of such a strategy will depend on the adaptation capacity of populations, which can partially be evaluated by the ecophysiological response of different genetic sources along a climatic gradient. This adaptation capacity results from the compromise between (i) the degree of genetic adaptation of seed sources to their environment of origin and (ii) the phenotypic plasticity of functional trait which can make it possible for transferred seed sources to positively respond to new growing conditions. We examined phenotypic variation in morphophysiological traits of six seed sources of white spruce (Picea glauca [Moench] Voss) along a regional climatic gradient in Québec, Canada. Seedlings from the seed sources were planted at three forest sites representing a mean annual temperature (MAT) gradient of 2.2°C. During the second growing season, we measured height growth (H2014) and traits related to resources use efficiency and photosynthetic rate (A max). All functional traits showed an adaptive response to the climatic gradient. Traits such as H2014, A max, stomatal conductance (g s ), the ratio of mesophyll to stomatal conductance, water use efficiency, and photosynthetic nitrogen-use efficiency showed significant variation in both physiological plasticity due to the planting site and seed source variation related to local genetic adaptation. However, the amplitude of seed source variation was much less than that related to plantation sites in the area investigated. The six seed sources showed a similar level of physiological plasticity. H2014, A max and g s , but not carboxylation capacity (V

  14. Airborne fungal colonisation of coarse woody debris in North Temperate Picea abies forest: impact of season and local spatial scale.

    PubMed

    Vasiliauskas, Rimvydas; Lygis, Vaidotas; Larsson, Karl-Henrik; Stenlid, Jan

    2005-04-01

    Coarse woody debris is important for mycodiversity in forest ecosystems, but its availability in managed stands is reduced. Leaving dead wood during felling is suggested as an option to sustain and restore the diversity. However, little is known what fungi would colonise freshly cut wood left on managed sites, and how the colonisation process is influenced by ecological factors. During summer and autumn, 120 freshly cut Picea abies stem sections over 8 cm in diameter were placed upright in mapped locations over two discrete plots separated by 100 m in a north-temperate forest. After seven weeks the sections were collected, and isolation and identification of fungi was done from their upper surfaces. In all 943 fungal strains were isolated, representing 97 species. Species richness in the summer survey was 42.5% higher than during the autumn survey. Low species similarity characterized the different seasons (Sorensen indices: S(S) = 0.36 and S(N) = 0.34) and for 21 species (22%) observation frequency was significantly affected by season. As a result, community structures in summer and autumn differed notably (z-test; P < 0.001). Species richness between the two plots differed by less than 10%, but there were 64 species (66%) found only in one of them, thus qualitative similarity was low (S(S) = 0.49). Quantitative similarity was higher (S(N) = 0.63), indicating that the dominant species colonised wood to a similar extent in both areas. Fungal community structure differed significantly among the two plots (z-test; P < 0.001). Our data showed that freshly cut CWD contributed to mycodiversity in managed north-temperate forest, providing habitats for numerous individuals from over 100 species. The fungal community within a single stand differed markedly both across small distances and over the seasons. In order to sustain and enhance mycodiversity in managed stands. coarse wood should always be left during harvesting. This study also demonstrates the importance of

  15. Tree growth and competition in an old-growth Picea abies forest of boreal Sweden: influence of tree spatial patterning

    USGS Publications Warehouse

    Fraver, Shawn; D'Amato, Anthony W.; Bradford, John B.; Jonsson, Bengt Gunnar; Jönsson, Mari; Esseen, Per-Anders

    2013-01-01

    Question: What factors best characterize tree competitive environments in this structurally diverse old-growth forest, and do these factors vary spatially within and among stands? Location: Old-growth Picea abies forest of boreal Sweden. Methods: Using long-term, mapped permanent plot data augmented with dendrochronological analyses, we evaluated the effect of neighbourhood competition on focal tree growth by means of standard competition indices, each modified to include various metrics of trees size, neighbour mortality weighting (for neighbours that died during the inventory period), and within-neighbourhood tree clustering. Candidate models were evaluated using mixed-model linear regression analyses, with mean basal area increment as the response variable. We then analysed stand-level spatial patterns of competition indices and growth rates (via kriging) to determine if the relationship between these patterns could further elucidate factors influencing tree growth. Results: Inter-tree competition clearly affected growth rates, with crown volume being the size metric most strongly influencing the neighbourhood competitive environment. Including neighbour tree mortality weightings in models only slightly improved descriptions of competitive interactions. Although the within-neighbourhood clustering index did not improve model predictions, competition intensity was influenced by the underlying stand-level tree spatial arrangement: stand-level clustering locally intensified competition and reduced tree growth, whereas in the absence of such clustering, inter-tree competition played a lesser role in constraining tree growth. Conclusions: Our findings demonstrate that competition continues to influence forest processes and structures in an old-growth system that has not experienced major disturbances for at least two centuries. The finding that the underlying tree spatial pattern influenced the competitive environment suggests caution in interpreting traditional tree

  16. Raman Spectroscopic Online Investigation of Respiratory Quotients in Pinus Sylvestris and Picea Abies during Drought and Shading

    NASA Astrophysics Data System (ADS)

    Hanf, S.; Fischer, S.; Hartmann, H.; Trumbore, S.; Popp, J.; Frosch, T.

    2014-12-01

    Drought and heat waves have been linked to forest mortality event across the globe. The underlying physiological processes are still not elucidated but both tree carbon and water relations have been identified as the driving forces. While studies on tree hydraulics are straightforward, studies on the tree carbon balance are not. For example, the use of different carbon compounds for maintenance respiration during drought cannot be assessed with measurements of carbon pools but requires real-time analyses of respiration stoichiometry. However, so far there were no technical solutions for such applications. Here we introduce cavity-enhanced Raman spectrometry (CERS) for simultaneous real-time monitoring of O2 and CO2 and rapid and continuous quantification of dark respiration rates and the respiratory quotient (RQ), i.e. the ratio of CO2 produced over O2 consumed during respiration. This ratio indicates the proportions of different substrates (carbohydrates [COH], lipids, proteins) used during respiration and allows fundamental insights into tree physiology. CERS combines high temporal resolution with a high dynamic concentration range for all important gases, ranging from few ppm to 100 vol. % with a single measurement every few seconds. The respiration analysis of tree branches was performed in a closed chamber for two species of different drought tolerance, Pinus sylvestris and Picea abies. We applied not only drought but also a shading treatment because both cause reductions in carbon assimilation rates but have different effects on tree hydraulics. Declines in RQ during shading in both species indicate a switch from pure COH metabolism to a mixture of COH, lipids and proteins. During drought such declines occurred only in the drought-tolerant pine but not in spruce and the underlying more dynamic carbon use strategy in pine may provide a physiological basis for its drought tolerance, more detailed investigation still pending. Our study highlights the suitability

  17. Genetic Adaptation vs. Ecophysiological Plasticity of Photosynthetic-Related Traits in Young Picea glauca Trees along a Regional Climatic Gradient

    PubMed Central

    Benomar, Lahcen; Lamhamedi, Mohammed S.; Rainville, André; Beaulieu, Jean; Bousquet, Jean; Margolis, Hank A.

    2016-01-01

    Assisted population migration (APM) is the intentional movement of populations within a species range to sites where future environmental conditions are projected to be more conducive to growth. APM has been proposed as a proactive adaptation strategy to maintain forest productivity and to reduce the vulnerability of forest ecosystems to projected climate change. The validity of such a strategy will depend on the adaptation capacity of populations, which can partially be evaluated by the ecophysiological response of different genetic sources along a climatic gradient. This adaptation capacity results from the compromise between (i) the degree of genetic adaptation of seed sources to their environment of origin and (ii) the phenotypic plasticity of functional trait which can make it possible for transferred seed sources to positively respond to new growing conditions. We examined phenotypic variation in morphophysiological traits of six seed sources of white spruce (Picea glauca [Moench] Voss) along a regional climatic gradient in Québec, Canada. Seedlings from the seed sources were planted at three forest sites representing a mean annual temperature (MAT) gradient of 2.2°C. During the second growing season, we measured height growth (H2014) and traits related to resources use efficiency and photosynthetic rate (Amax). All functional traits showed an adaptive response to the climatic gradient. Traits such as H2014, Amax, stomatal conductance (gs), the ratio of mesophyll to stomatal conductance, water use efficiency, and photosynthetic nitrogen-use efficiency showed significant variation in both physiological plasticity due to the planting site and seed source variation related to local genetic adaptation. However, the amplitude of seed source variation was much less than that related to plantation sites in the area investigated. The six seed sources showed a similar level of physiological plasticity. H2014, Amax and gs, but not carboxylation capacity (Vcmax), were

  18. Targeted proteomics using selected reaction monitoring reveals the induction of specific terpene synthases in a multi-level study of methyl jasmonate-treated Norway spruce (Picea abies).

    PubMed

    Zulak, Katherine G; Lippert, Dustin N; Kuzyk, Michael A; Domanski, Dominik; Chou, Tina; Borchers, Christoph H; Bohlmann, Jörg

    2009-12-01

    Induction of terpene synthase (TPS) gene expression and enzyme activity is known to occur in response to various chemical and biological stimuli in several species of spruce (genus Picea). However, high sequence identity between TPS family members has made it difficult to determine the induction patterns of individual TPS at the protein and transcript levels and whether specific TPS enzymes respond differentially to treatment. In the present study we used a multi-level approach to measure the induction and activity of TPS enzymes in protein extracts of Norway spruce (Picea abies) bark tissue following treatment with methyl jasmonate (MeJA). Measurements were made on the transcript, protein, enzyme activity and metabolite levels. Using a relatively new proteomics application, selective reaction monitoring (SRM), it was possible to differentiate and quantitatively measure the abundance of several known TPS proteins and three 1-deoxy-D-xylulose 5-phosphate synthase (DXS) isoforms in Norway spruce. Protein levels of individual TPS and DXS enzymes were differentially induced upon MeJA treatment and good correlation was generally observed between induction of transcripts, proteins, and enzyme activities. Most of the mono- and diterpenoid metabolites accumulated with similar temporal patterns of induction as part of the coordinated multi-compound chemical defense response. Protein and enzyme activity levels of the monoTPS (+)-3-carene synthase and the corresponding accumulation of (+)-3-carene was induced to a higher fold change than any other TPS or metabolite measured, indicating an important role in the induced terpenoid defense response in Norway spruce.

  19. The influence of O3, NO2 and SO2 on growth of Picea abies and Fagus sylvatica in the Carpathian Mountains.

    PubMed

    Muzika, R M; Guyette, R P; Zielonka, T; Liebhold, A M

    2004-07-01

    At 17 long-term pollution monitoring sites throughout the Carpathian Mountains, tree growth patterns and variation in growth rate were examined to determine relationship of tree growth to specific pollutants. Canopy dominant Picea abies and Fagus sylvatica were selected at each site. Basal area increment (BAI) values were calculated from raw ring widths and used as an estimate of tree growth. Across all sites, BAI chronologies were highly variable, therefore local conditions and forest structure accounted for considerable variation. Several significant relationships, however, implicated a role of pollutants on tree growth. Average levels (1997-1999) of NO(2) and SO(2) were inversely related to BAI means (1989-1999). Although average O(3) alone was not related to growth, the maximum O(3) value reported at the sites was negatively correlated with overall growth. A variable representing the combined effect of O(3), NO(2) and SO(2) was negatively correlated with both P. abies and F. sylvatica growth. Pollution data were used to categorize all sites into 'high' or 'low' pollution sites. Difference chronologies based on these categories indicated trends of decline in the 'high' pollution sites relative to 'low' pollution site. In the more heavily polluted sites, the BAI of Fagus sylvatica has declined approximately 50% and Picea abies has declined 20% over the past 45 years.

  20. Phylogeny of Phaeomollisia piceae gen. sp. nov.: a dark, septate, conifer-needle endophyte and its relationships to Phialocephala and Acephala.

    PubMed

    Grünig, Christoph R; Queloz, Valentin; Duò, Angelo; Sieber, Thomas N

    2009-02-01

    Dark, septate endophytes (DSE) were isolated from roots and needles of dwarf Picea abies and from roots of Vaccinium spp. growing on a permafrost site in the Jura Mountains in Switzerland. Two of the isolates sporulated after incubation for more than one year at 4 degrees C. One of them was a hitherto undescribed helotialean ascomycete Phaeomollisia piceae gen. sp. nov., the other was a new species of Phialocephala, P. glacialis sp. nov. Both species are closely related to DSE of the Phialocephala fortinii s. lat.-Acephala applanata species complex (PAC) as revealed by phylogenetic analyses of the ITS and 18S rDNA regions. Morphologically dissimilar fungi, such as Vibrissea and Loramyces species, are phylogenetically also closely linked to the new species and the PAC. Cadophora lagerbergii and C. (Phialophora) botulispora are moved to Phialocephala because Phialocephala dimorphospora and P. repens are the closest relatives. Several Mollisia species were closely related to the new species and the PAC according to ITS sequence comparisons. One DSE from needles of Abies alba and one from shoots of Castanea sativa formed Cystodendron anamorphs in culture. Their identical 18S sequences and almost identical ITS sequences indicated Mollisia species as closest relatives, suggesting that Mollisia species are highly euryoecious.

  1. Rifting process of the Izu-Ogasawara-Mariana arc-backarc system inferred from active source seismic studies

    NASA Astrophysics Data System (ADS)

    Takahashi, N.; Kodaira, S.; Miura, S.; Sato, T.; Yamashita, M.; No, T.; Takizawa, K.; Kaiho, Y.; Kaneda, Y.

    2008-12-01

    The Izu-Ogasawara-Mariana (IBM) arc-backarc system has continued the crustal growth through crustal thickening by magmatic activities and crustal thinning by backarc opening. Tatsumi et al (2008) proposed petrological crustal growth model started from basaltic magmas rising from the slab, and showed the consistency with the seismic velocity model. Although crustal growth by the crustal thickening are modeled, crustal structural change by the backarc opening are not still unknown yet. The Shikoku Basin and Parece Vela Basin were formed by the backarc opening during approximately 15-30 Ma. Since 6 Ma, the Mariana Trough has opened and the stage already moved to spreading process from rifting process. In the northern Izu-Ogasawara arc, the Sumisu rift is in the initial rifting stage. Therefore, understanding of the crustal change by the backarc opening from rifting to spreading is indispensable to know the crustal growth of whole Izu-Ogasawara-Mariana island arc. Japan Agency for Marine-Earth Science and Technology (JAMSTEC) has carried out seismic studies using a multichannel reflection survey system and ocean bottom seismographs (OBSs) around the IBM arc since 2003 (Takahashi et al., 2007; Kodaira et al., 2007; Takahashi et al., 2008; Kodaira et al., 2008). We already obtained eight P-wave velocity models across the IBM arc and these structures record the crustal structural change during the backarc opening process from the rifting stage to the spreading stage. As the results, we identified characteristics of the crustal structural change accompanied with backarc opening as follows. (1) Beneath the initial rifting stage without normal faults, for example, in the northern tip of the Mariana Trough, crustal thickening are identified. (2) Beneath the initial rifting stage with normal faults, for example, in the Sumisu Rift, the crustal thickness is almost similar to that beneath the volcanic front. Although an existence of the crust-mantle transition layer with

  2. Sediment wave-forms and modes of construction on Mariana (and other) intra-oceanic arc volcanoes

    NASA Astrophysics Data System (ADS)

    Embley, R. W.; Stern, R. J.; Chadwick, B.; Tamura, Y.; Merle, S. G.

    2014-12-01

    Most intra-oceanic arc volcanoes are composite edifices constructed primarily in the submarine environment, built up by volcaniclastic sediments derived from hydroclastic and pyroclastic processes at/near the summits, punctuated by occasional lava flows and intrusions. Of particular interest in the mode of construction are extensive fields of large sediment waveforms (SWFs), up to >2 km wavelength and >100 m amplitude, on the submarine flanks of many islands and seamounts within the Mariana and other intra-oceanic subduction zones. These SWFs are composed of coarse-grained volcaniclastic sediments derived from the (approximate) point source summits of the island and submarine volcanoes. SWFs around some seamounts and islands, particularly those with large calderas, define quasi-concentric ring-like ridges, suggesting formation by density currents generated during submarine and island eruptions, and preserved for 10s of thousands of years. Some types of SWFs appear to have formed by progressive slumping of oversteepened slopes without fluidization. General conclusions about the origin of SWFs are hampered by the dearth of samples and high resolution seismic reflection profiles. However, large coherent slumps and debris avalanches documented for some ocean islands (e.g., Hawaiian Islands) are (mostly) are not as evident on the composite arc volcanoes. Submarine Mariana arc (and other intra-oceanic arc) volcanism probably spread volcaniclastic material primarily during submarine "Neptunian" eruptions and by progressive slides and other sediment flow rather than by catastrophic flank collapse. These processes could mitigate the Hawaiian-style of tsumami hazard, but Krakatoa-type tsunami hazards exist.

  3. P and S velocity tomography of the Mariana subduction system from a combined land-sea seismic deployment

    NASA Astrophysics Data System (ADS)

    Barklage, Mitchell; Wiens, Douglas A.; Conder, James A.; Pozgay, Sara; Shiobara, Hajime; Sugioka, Hiroko

    2015-03-01

    Seismic imaging provides an opportunity to constrain mantle wedge processes associated with subduction, volatile transport, arc volcanism, and back-arc spreading. We investigate the seismic velocity structure of the upper mantle across the Central Mariana subduction system using data from the 2003-2004 Mariana Subduction Factory Imaging Experiment, an 11 month deployment consisting of 20 broadband seismic stations installed on islands and 58 semibroadband ocean bottom seismographs. We determine the three-dimensional VP and VP/VS structure using over 25,000 local and over 2000 teleseismic arrival times. The mantle wedge is characterized by slow velocity and high VP/VS beneath the fore arc, an inclined zone of slow velocity underlying the volcanic front, and a strong region of slow velocity beneath the back-arc spreading center. The slow velocities are strongest at depths of 20-30 km in the fore arc, 60-70 km beneath the volcanic arc, and 20-30 km beneath the spreading center. The fore-arc slow velocity anomalies occur beneath Big Blue seamount and are interpreted as resulting from mantle serpentinization. The depths of the maximum velocity anomalies beneath the arc and back arc are nearly identical to previous estimates of the final equilibrium depths of mantle melts from thermobarometry, strongly indicating that the low-velocity zones delineate regions of melt production in the mantle. The arc and back-arc melt production regions are well separated at shallow depths, but may be connected at depths greater than 80 km.

  4. Environmental Assessment: Farallon De Medinilla and Tinian Military Lease Areas, Commonwealth of the Northern Mariana Islands, Integrated Natural Resources Management Plan

    DTIC Science & Technology

    2004-07-01

    Miles 200 FDM Farallon de Pajaros (Uracas) Maug Asuncion Agrihan Pagan Alamagan Guguan Sarigan Anatahan Farallon de Medinilla (FDM) Saipan Tinian...eradication of feral ungulates that destroy Micronesian megapode habitats on the islands of Sarigan and Anatahan (Figure 1). The specific projects...elsewhere in the Marianas (e.g., Sarigan and Anatahan ). X X X X 13 Species Survey, FDM Annual marine surveys of marine environs around FDM to access impacts

  5. Energy Transition Initiative: Island Energy Snapshot - Commonwealth of the Northern Mariana Islands; U.S. Department of Energy (DOE), NREL (National Renewable Energy Laboratory)

    SciTech Connect

    2015-06-01

    This profile provides a snapshot of the energy landscape of the Commonwealth of the Northern Mariana Islands (CNMI), a commonwealth in political union with the United States that is located in the northern Pacific Ocean. CNMI’s electricity rates for residential customers range from $0.19 to $0.33 U.S. dollars (USD) per kilowatt-hour (kWh), above the average U.S. residential rate of $0.13 USD/kWh.

  6. The birth and death of the oceanic lithosphere: Geochemical and tectonic investigations of the Juan de Fuca Ridge and Mariana Trench

    NASA Astrophysics Data System (ADS)

    Hulme, Samuel M.

    The research I conducted for my dissertation addresses specifically: (1) the role of fluid alteration of the ocean crust during its maturing stages near a spreading center using the Juan de Fuca Ridge as the model; and (2) the role of fluids in the demise of the crust as it is recycled within a subduction zone, using the non-accretioanry Mariana system as the model. The first 2 chapters detail the progressive alteration of a region of young oceanic crust (at the Juan de Fuca Ridge) by combining the results of multiple deep-sea drilling legs, long-term borehole observatory sampling, and hydrothermal vent chemistry. The third chapter examines fluid transfer during serpentinization of the suprasubduction lithosphere, based on the relationship between trace-element patterns and serpentine phases in serpentinite mud pore fluids and serpentinite mud/rocks collected at serpentinite mud volcanoes on the Mariana forearc. The final chapter presents detailed bathymetric and Hybrid Remote Operated Vehicle (HROV) Nereus surveys conducted in the deepest regions of the Mariana Trench (and therefore the world) that are interpreted to explain the regional tectonic processes and to guide future exploration efforts. These new efforts may lead to further discoveries of unique geologic features that will allow researchers to better understand the ongoing evolution of the Earth.

  7. No slab-derived CO2 in Mariana Trough back-arc basalts: Implications for carbon subduction and for temporary storage of CO2 beneath slow spreading ridges

    NASA Astrophysics Data System (ADS)

    MacPherson, Colin G.; Hilton, David R.; Hammerschmidt, Konrad

    2010-11-01

    The Southern Mariana Trough is particularly well suited to study mass balance in subduction zones because the flux of material recycled from the subducted slab has been shown to diminish to negligible levels in the southernmost part of the area. We present new He and Ar concentration and isotopic data for 16 back-arc basaltic glasses and combine these with previously published CO2 and H2O concentration and δ13C data to explore the recycling of carbon and light noble gases in the Mariana back arc. Degassing has affected all samples and is particularly extensive in more water-rich samples, i.e., those containing the largest recycled component. The degassing history features three stages: (1) deep degassing which commenced when the melt reached saturation of CO2 and noble gases in the mantle, (2) preeruptive degassing during storage in the crust-mantle transition zone which involved addition of extraneous CO2 to the vapor phase, and (3) eruption. CO2 released during stage 1 was, at least partially, incorporated into wall rock and subsequently remobilized during stage 2 degassing of later magma batches. Reconstructed parental values for 3He/4He, δ13C, CO2/3He, and CO2/40Ar* are indistinguishable from those of mid-ocean ridge basalt. This implies that there is negligible recycling of subducted carbon, helium, or argon into the source of Mariana Trough basalt.

  8. Peridotites from a ductile shear zone within back-arc lithospheric mantle, southern Mariana Trench: Results of a Shinkai 6500 dive

    NASA Astrophysics Data System (ADS)

    Michibayashi, K.; Ohara, Y.; Stern, R. J.; Fryer, P.; Kimura, J.-I.; Tasaka, M.; Harigane, Y.; Ishii, T.

    2009-05-01

    Two N-S fault zones in the southern Mariana fore arc record at least 20 km of left-lateral displacement. We examined the eastward facing slope of one of the fault zones (the West Santa Rosa Bank fault) from 6469 to 5957 m water depth using the submersible Shinkai 6500 (YK06-12 Dive 973) as part of a cruise by the R/V Yokosuka in 2006. The dive recovered residual but still partly fertile lherzolite, residual lherzolite, and dunite; the samples show mylonitic, porphyroclastic, and coarse, moderately deformed secondary textures. Crystal-preferred orientations of olivine within the peridotites show a typical [100](010) pattern, with the fabric intensity decreasing from rocks with coarse secondary texture to mylonites. The sampled peridotites therefore represent a ductile shear zone within the lithospheric mantle of the overriding plate. Peridotites were probably exposed in association with a tear in the subducting slab, previously inferred from bathymetry and seismicity. Furthermore, although the dive site is located in the fore arc close to the Mariana Trench, spinel compositions within the sampled peridotites are comparable to those from the Mariana Trough back arc, suggesting that back-arc basin mantle is exposed along the West Santa Rosa Bank fault.

  9. The source of the subduction component in convergent margin magmas: Trace element and radiogenic isotope evidence from Eocene boninites, Mariana forearc

    SciTech Connect

    Stern, R.J. ); Morris, J. ); Bloomer, S.H. ); Hawkins, J.W Jr. )

    1991-05-01

    Boninites are generally accepted as being melts from mixtures of depleted harzburgite and a water- and incompatible element-enriched component thought to be derived from the subducted plate (the subduction component). From calculations in this study, Mariana boninites are inferred to obtain 70-90% of Sr, 60-95% of Pb, and 0-80% of Nd from the subduction component, and so provide unique insights into the composition and source of this material as sampled early in the development of the arc. Nd-, Pb-, and Sr-isotopic compositions of Eocene boninites from three dredge sites in the Mariana forearc indicate that this subduction component is isotopically indistinguishable from mantle sources responsible for the generation of typical, northern hemisphere ocean-island basalt. Initial {sup 87}Sr/{sup 86}Sr {epsilon}-Nd-, and Pb-isotopic compositions fall within the Sr-Nd mantle array and along the NHRL for Pb-isotopic compositions. The values for the Eocene boninites are very similar to those of modern Mariana arc lavas, indicating that the subduction component is isotopically homogeneous in time and space. If the depleted endmember in boninite petrogenesis is assumed to be a MORB-source, subducted sediments cannot be significant sources of the subduction component. Instead, the subduction component identified for these boninites must have been derived from dehydration of subducted basaltic crust or via re-equilibration of fluids - and cations - released from the dehydrating slab with the overlying mantle wedge.

  10. Magnetic Structure of Backarc Spreading Axis with Hydrothermal Vents; the Southern Mariana Trough

    NASA Astrophysics Data System (ADS)

    Fujii, M.; Okino, K.; Mochizuki, N.; Honsho, C.; Szitkar, F.; Dyment, J.; Nakamura, K.

    2012-12-01

    Seafloor hydrothermal systems are important in relation to global heat and chemical fluxes as well as habitat of microbial communities. The substantial variation of hydrothermal systems in various tectonic settings has important implications for the magnetic structure of oceanic crust. It has been very difficult to detect the geophysical signature of hydrothermal systems from sea-surface data because the small scale of hydrothermal systems is below the limit of resolution. The advance of near-bottom survey methods using a submersible, deep-tow, ROV and AUV has made possible high-resolution geophysical mapping around hydrothermal areas. Near-bottom magnetic surveys can provide direct information on the magnetization of the shallower oceanic crust, implying hydrothermal alteration both in active and fossil vent sites. Near-bottom three component magnetic measurements on submersible Shinkai 6500 were carried out at hydrothermal fields in the Southern Mariana Trough, a slow spreading backarc basin. Fourteen dive surveys were conducted during cruises YK11-10 and YK10-11. We investigated the magnetic structure of four hydrothermal systems located at on- and off-axis to clarify how the geophysical and geological setting controls the fluid circulation at small scale. Recent researches at slow spreading ridges showed a relationship between crustal magnetic structure and host rock around hydrothermal vents (e.g. Tivey and Dyment, 2010), but no observation at backarc spreading axis has been reported so far. We carefully corrected the effects of induced and permanent magnetizations of the submersible by applying the method of Isezaki [1986] with dumped least-square method (Honsho et al., 2009). After subtracting the IGRF from the corrected observed data, we obtained geomagnetic vector anomalies in geographical coordinate. For three transects of the axis, we applied three methods; 2D inversion technique (Parker and Huestis, 1972), 2D forward modeling technique (Honsho et al

  11. Bubble Plumes above erupting NW Rota-1 submarine volcano, Mariana Arc

    NASA Astrophysics Data System (ADS)

    Chadwick, B.; Merle, S. G.; Embley, R. W.; Buck, N.; Resing, J. A.; Leifer, I.

    2013-12-01

    NW Rota-1 is a submarine volcano in the Mariana volcanic arc with a summit depth of 517 m, located ~100 km north of Guam. Underwater explosive eruptions driven by magmatic gases were first witnessed here in 2004 and the volcano has remained persistently active ever since. During a March 2010 expedition to NW Rota-1 with the remotely operated vehicle Jason, we observed intermittent explosive activity at five distinct eruptive vents along a line 100-m long near the summit of the volcano (550-590 m depth). The continuous but variable eruptive activity produced CO2 bubble plumes that rose in the water column over the volcano and could be readily imaged by sonar because they provide excellent acoustic reflectors. This study compares the manifestations of NW Rota's eruptive activity as measured by several independent methods, including: (1) an EM122 multibeam sonar system (12 kHz) on the R/V Kilo Moana that imaged bubble plumes in the water column over the volcano, (2) hydrophone data that recorded the sounds of the variable eruptive activity, and (3) visual observations of the activity at the eruptive vents on the seafloor from Jason. Throughout the 2010 expedition numerous passes were made over the volcano's summit to image the bubble plumes with the EM122 multibeam sonar, in order to capture the variability of the plumes over time and to relate them to the eruptive output of the volcano. The mid-water sonar dataset totals >95 hours of observations over a 12-day period. Analysis of the EM122 dataset shows: (1) bubble plumes were visible in the water column on every pass over the summit, (2) separate plumes were resolvable from up to 4 of the 5 eruptive vents at times, (3) plume heights and intensities were variable with time, (4) the highest observed bubble plume rise height was 415 meters above the seafloor to within 175 m of the ocean surface, while lower amplitude wisps rose to heights <100 m from the surface, (5) most of the bubble plumes were deflected to the WSW

  12. Mission Immiscible: Distinct subduction components generate two primary magmas of Pagan Volcano, Mariana arc

    NASA Astrophysics Data System (ADS)

    Tamura, Y.; Ishizuka, O.; Stern, R. J.; Nunokawa, A.; Shukuno, H.; Kawabata, H.; Hirahara, Y.; Chang, Q.; Miyazaki, T.; Kimura, J.; Embley, R. W.; Bloomer, S. H.; Tatsumi, Y.

    2012-12-01

    Pagan is one of the largest (2,160 km3; Bloomer et al., 1989) volcanoes along the Mariana arc magmatic front. Pagan has a maximum elevation of 570 m (Mt. Pagan), but its submarine flanks descend to 2,000-3,000 m, and most of the volcano is submarine and unexplored. Bathymetric mapping and ROV Hyper-Dolphin (HPD1147) dive on the NE submarine flank of Pagan were carried out during NT10-12 (R/V Natsushima) in July 2010. There are no systematic differences between subaerial and submarine lavas with > 52 wt % SiO2, suggesting derivation from the same magmatic system. Twenty least-fractionated basalts (48.5-50 wt % SiO2) extend to higher MgO (10-11 wt %) and Mg# (66-70) than subaerial lavas. Compositions of olivine (up to Fo94) and spinels (Cr# up to 0.8) suggest that Pagan primitive magmas formed from high degrees of mantle melting. Two geochemical groups of basalts can be distinguished at similar 10-11 wt % MgO; these erupted about the same time, 500 m apart. Both contain clinopyroxene and olivine phenocrysts, thus, these two groups are referred to as COB1 and COB2. Lower TiO2, FeO, Na2O, K2O, incompatible trace element abundances, and Nb/Yb suggest that COB1 formed from higher degrees of mantle melting. In addition, LREE-enrichment and higher Th/Nb in COB2 contrast with LREE-depletion and lower Th/Nb in COB1. Higher Ba/Th and Ba/Nb and lower Th/Nb indicate that main subduction addition in COB1 was dominated by hydrous fluid, whereas that in COB2 was dominated by sediment melt. Sr-Nd-Pb-Hf isotopes are also consistent with this scenario. Importantly, the subduction addition, that caused more melting of the COB1 source was mostly hydrous fluid. In contrast to Pagan, we observed two primary magmas (COB and POB) in the NW Rota-1 volcano (NWR1), ~40 km behind the volcanic front. NWR1 COB has a greater subduction component, both hydrous fluid and sediment melt, than POB, perhaps reflecting that the subducting slab below NWR1 is > 100 km deeper than that beneath Pagan. At

  13. A theoretical framework for whole-plant carbon assimilation efficiency based on metabolic scaling theory: a test case using Picea seedlings.

    PubMed

    Wang, Zhiqiang; Ji, Mingfei; Deng, Jianming; Milne, Richard I; Ran, Jinzhi; Zhang, Qiang; Fan, Zhexuan; Zhang, Xiaowei; Li, Jiangtao; Huang, Heng; Cheng, Dongliang; Niklas, Karl J

    2015-06-01

    Simultaneous and accurate measurements of whole-plant instantaneous carbon-use efficiency (ICUE) and annual total carbon-use efficiency (TCUE) are difficult to make, especially for trees. One usually estimates ICUE based on the net photosynthetic rate or the assumed proportional relationship between growth efficiency and ICUE. However, thus far, protocols for easily estimating annual TCUE remain problematic. Here, we present a theoretical framework (based on the metabolic scaling theory) to predict whole-plant annual TCUE by directly measuring instantaneous net photosynthetic and respiratory rates. This framework makes four predictions, which were evaluated empirically using seedlings of nine Picea taxa: (i) the flux rates of CO(2) and energy will scale isometrically as a function of plant size, (ii) whole-plant net and gross photosynthetic rates and the net primary productivity will scale isometrically with respect to total leaf mass, (iii) these scaling relationships will be independent of ambient temperature and humidity fluctuations (as measured within an experimental chamber) regardless of the instantaneous net photosynthetic rate or dark respiratory rate, or overall growth rate and (iv) TCUE will scale isometrically with respect to instantaneous efficiency of carbon use (i.e., the latter can be used to predict the former) across diverse species. These predictions were experimentally verified. We also found that the ranking of the nine taxa based on net photosynthetic rates differed from ranking based on either ICUE or TCUE. In addition, the absolute values of ICUE and TCUE significantly differed among the nine taxa, with both ICUE and temperature-corrected ICUE being highest for Picea abies and lowest for Picea schrenkiana. Nevertheless, the data are consistent with the predictions of our general theoretical framework, which can be used to access annual carbon-use efficiency of different species at the level of an individual plant based on simple, direct

  14. Evolution of West Rota Volcano in the Southern Mariana Arc: Evidence from Swathmapping, Seafloor Robotics, and 40Ar/39Ar Geochronology

    NASA Astrophysics Data System (ADS)

    Stern, R. J.; Tamura, Y.; Embley, R. W.; Ishizuka, O.; Merle, S.; Basu, N. K.; Kawabata, H.; Bloomer, S. H.

    2006-12-01

    West Rota volcano (WRV) is a large (25 km base), extinct submarine volcano in the southern Mariana arc. Its shallowest point lies 300m bsl; before caldera collapse WRV probably was a small island. Several bathymetric and sonar backscatter mapping campaigns reveal a large caldera, 6 x 10 km in diameter, with a maximum of 1km relief. WRV lies near the northern termination of a major NNE-trending normal fault. This and a second, parallel fault just west of the volcano separate uplifted, thick crust beneath the frontal arc to the east from subsiding, thin back-arc basin crust to the west. The youthful morphology of basin-margin faults indicate that the southern Mariana arc is tectonically active. Compared to other Mariana arc volcanoes, WRV is remarkable for 4 reasons: 1) It consists of a lower, predominantly andesite section overlain by a bimodal rhyodacite-basalt layered sequence; 2) Andesitic rocks are locally intensely altered and mineralized; 3) It has a large caldera; and 4) WRV is built on a major fault. Large calderas are commonly associated with volcanoes that erupt voluminous felsic lava (WRV rhyodacite pumice contains 72% SiO2). Such volcanoes are common in the Izu and Kermadec arcs but are otherwise unknown from the Marianas and other primitive, intra- oceanic arcs. WRV's caldera diameter of 6x10 km is large compared with Izu and Kermadec felsic calderas. Robotic seafloor examination has concentrated on understanding the volcanic history exposed in the caldera walls. One dive was carried out with ROPOS during TT167 in April 2004 (R785), followed by 4 dives with Hyperdolphin 3K during NT0517 in Oct. 2005 (HD482-484, 489). 40Ar/39Ar dating indicates that andesitic volcanism formed the lower volcanic section ca. 330,000-550,000 years ago, whereas eruption of the upper rhyodacites and basalts occurred 37,000-51,000 years ago. Four sequences of rhyodacite pyroclastics each are 20-75m thick, are unwelded, and show reverse grading, indicating submarine eruption of

  15. The mycorrhizal fungus Amanita muscaria induces chitinase activity in roots and in suspension-cultured cells of its host Picea abies.

    PubMed

    Sauter, M; Hager, A

    1989-08-01

    A cell-wall fraction of the mycorrhizal fungus Amanita muscaria increased the chitinase activity in suspension-cultured cells of spruce (Picea abies (L.) Karst.) which is a frequent host of Amanita muscaria in nature. Chitinase activity was also increased in roots of spruce trees upon incubation with the fungal elicitor. Non-induced levels of chitinase activity in spruce were higher in suspension cells than in roots whereas the elicitorinduced increase of chitinase activity was higher in roots. Treatment of cells with hormones (auxins and cytokinin) resulted in a severalfold depression of enzyme activity. However, the chitinase activity of hormone-treated as well as hormone-free cells showed an elicitor-induced increase. Suspension cells of spruce secreted a large amount of enzyme into the medium. It is postulated that chitinases released from the host cells in an ectomycorrhizal system partly degrade the fungal cell walls, thus possibly facilitating the exchange of metabolites between the symbionts.

  16. A life cycle model to enable research of cryostorage recalcitrance in temperate woody species: the case of sitka spruce (Picea sitchensis).

    PubMed

    Gale, S; Benson, E E; Harding, K

    2013-01-01

    Empirical testing of protocols and fundamental investigations are the approaches usually applied to study germplasm storage recalcitrance in temperate plants. However, they can fall short of practicable solutions, even after exhaustive experimentation, and the generation of negative survival data makes it difficult to plan further investigations. Picea sitchensis somatic embryos are amenable to cryopreservation whereas in vitro shoot meristems, although able to survive, are incapable of sustained recovery. Differential Scanning Calorimetry (DSC) revealed that these disparate responses could not be attributed to biophysical factors. A model is presented hypothesising that in some cases life cycle adaptations (cold hardening, dormancy) may have opposing influences on survival causing delayed-onset, cryogenically-induced loss of viability in temperate tree species.

  17. PSII photochemistry in vegetative buds and needles of Norway spruce (Picea abies L. Karst.) probed by OJIP chlorophyll a fluorescence measurement.

    PubMed

    Katanić, Zorana; Atić, Lejla; Ferhatović, Dž; Cesar, Vera; Lepeduš, H

    2012-06-01

    Vegetative buds represent developmental stage of Norway spruce (Picea abies L. Karst.) needles where chloroplast biogenesis and photosynthetic activity begin. We used the analyses of polyphasic chlorophyll a fluorescence rise (OJIP) to compare photosystem II (PSII) functioning in vegetative buds and fully photosynthetically active mature current-year needles. Considerably decreased performance index (PIABS) in vegetative buds compared to needles pointed to their low photosynthetic efficiency. Maximum quantum yield of PSII (Fv/Fm) in buds was slightly decreased but above limited value for functionality indicating that primary photochemistry of PSII is not holdback of vegetative buds photosynthetic activity. The most significant difference observed between investigated developmental stages was accumulation of reduced primary quinine acceptor of PSII (QA-) in vegetative buds, as a result of its limited re-oxidation by passing electrons to secondary quinone acceptor, QB. We suggest that reduced electron transfer from QA- to QB could be the major limiting factor of photosynthesis in vegetative buds.

  18. Landscape of Fluid Sets of Hairpin-Derived 21-/24-nt-Long Small RNAs at Seed Set Uncovers Special Epigenetic Features in Picea glauca

    PubMed Central

    2017-01-01

    Conifers’ exceptionally large genome (20–30 Gb) is scattered with 60% retrotransposon (RT) components and we have little knowledge on their origin and evolutionary implications. RTs may impede the expression of flanking genes and provide sources of the formation of novel small RNA (sRNAs) populations to constrain events of transposon (TE) proliferation/transposition. Here we show a declining expression of 24-nt-long sRNAs and low expression levels of their key processing gene, pgRTL2 (RNASE THREE LIKE 2) at seed set in Picea glauca. The sRNAs in 24-nt size class are significantly less enriched in type and read number than 21-nt sRNAs and have not been documented in other species. The architecture of MIR loci generating highly expressed 24-/21-nt sRNAs is featured by long terminal repeat—retrotransposons (LTR-RTs) in families of Ty3/Gypsy and Ty1/Copia elements. This implies that the production of sRNAs may be predominantly originated from TE fragments on chromosomes. Furthermore, a large proportion of highly expressed 24-nt sRNAs does not have predictable targets against unique genes in Picea, suggestive of their potential pathway in DNA methylation modifications on, for instance, TEs. Additionally, the classification of computationally predicted sRNAs suggests that 24-nt sRNA targets may bear particular functions in metabolic processes while 21-nt sRNAs target genes involved in many different biological processes. This study, therefore, directs our attention to a possible extrapolation that lacking of 24-nt sRNAs at the late conifer seed developmental phase may result in less constraints in TE activities, thus contributing to the massive expansion of genome size. PMID:28082604

  19. The May 2003 eruption of Anatahan volcano, Mariana Islands: Geochemical evolution of a silicic island-arc volcano

    USGS Publications Warehouse

    Wade, J.A.; Plank, T.; Stern, R.J.; Tollstrup, D.L.; Gill, J.B.; O'Leary, J. C.; Eiler, J.M.; Moore, R.B.; Woodhead, J.D.; Trusdell, F.; Fischer, T.P.; Hilton, David R.

    2005-01-01

    The first historical eruption of Anatahan volcano began on May 10, 2003. Samples of tephra from early in the eruption were analyzed for major and trace elements, and Sr, Nd, Pb, Hf, and O isotopic compositions. The compositions of these tephras are compared with those of prehistoric samples of basalt and andesite, also newly reported here. The May 2003 eruptives are medium-K andesites with 59-63 wt.% SiO2, and are otherwise homogeneous (varying less than 3% 2?? about the mean for 45 elements). Small, but systematic, chemical differences exist between dark (scoria) and light (pumice) fragments, which indicate fewer mafic and oxide phenocrysts in, and less degassing for, the pumice than scoria. The May 2003 magmas are nearly identical to other prehistoric eruptives from Anatahan. Nonetheless, Anatahan has erupted a wide range of compositions in the past, from basalt to dacite (49-66 wt.% SiO2). The large proportion of lavas with silicic compositions at Anatahan (> 59 wt.% SiO2) is unique within the active Mariana Islands, which otherwise erupt a narrow range of basalts and basaltic andesites. The silicic compositions raise the question of whether they formed via crystal fractionation or crustal assimilation. The lack of 87Sr/86Sr variation with silica content, the MORB-like ??18O, and the incompatible behavior of Zr rule out assimilation of old crust, altered crust, or zircon-saturated crustal melts, respectively. Instead, the constancy of isotopic and trace element ratios, and the systematic variations in REE patterns are consistent with evolution by crystal fractionation of similar parental magmas. Thus, Anatahan is a type example of an island-arc volcano that erupts comagmatic basalts to dacites, with no evidence for crustal assimilation. The parental magmas to Anatahan lie at the low 143Nd/144Nd, Ba/La, and Sm/La end of the spectrum of magmas erupted in the Marianas arc, consistent with 1-3 wt.% addition of subducted sediment to the mantle source, or roughly one

  20. Philippine Sea Plate inception, evolution, and consumption with special emphasis on the early stages of Izu-Bonin-Mariana subduction

    NASA Astrophysics Data System (ADS)

    Lallemand, Serge

    2016-12-01

    We compiled the most relevant data acquired throughout the Philippine Sea Plate (PSP) from the early expeditions to the most recent. We also analyzed the various explanatory models in light of this updated dataset. The following main conclusions are discussed in this study. (1) The Izanagi slab detachment beneath the East Asia margin around 60-55 Ma likely triggered the Oki-Daito plume occurrence, Mesozoic proto-PSP splitting, shortening and then failure across the paleo-transform boundary between the proto-PSP and the Pacific Plate, Izu-Bonin-Mariana subduction initiation and ultimately PSP inception. (2) The initial splitting phase of the composite proto-PSP under the plume influence at ˜54-48 Ma led to the formation of the long-lived West Philippine Basin and short-lived oceanic basins, part of whose crust has been ambiguously called "fore-arc basalts" (FABs). (3) Shortening across the paleo-transform boundary evolved into thrusting within the Pacific Plate at ˜52-50 Ma, allowing it to subduct beneath the newly formed PSP, which was composed of an alternance of thick Mesozoic terranes and thin oceanic lithosphere. (4) The first magmas rising from the shallow mantle corner, after being hydrated by the subducting Pacific crust beneath the young oceanic crust near the upper plate spreading centers at ˜49-48 Ma were boninites. Both the so-called FABs and the boninites formed at a significant distance from the incipient trench, not in a fore-arc position as previously claimed. The magmas erupted for 15 m.y. in some places, probably near the intersections between back-arc spreading centers and the arc. (5) As the Pacific crust reached greater depths and the oceanic basins cooled and thickened at ˜44-45 Ma, the composition of the lavas evolved into high-Mg andesites and then arc tholeiites and calc-alkaline andesites. (6) Tectonic erosion processes removed about 150-200 km of frontal margin during the Neogene, consuming most or all of the Pacific ophiolite

  1. The May 2003 eruption of Anatahan volcano, Mariana Islands: Geochemical evolution of a silicic island-arc volcano

    NASA Astrophysics Data System (ADS)

    Wade, Jennifer A.; Plank, Terry; Stern, Robert J.; Tollstrup, Darren L.; Gill, James B.; O'Leary, Julie C.; Eiler, John M.; Moore, Richard B.; Woodhead, Jon D.; Trusdell, Frank; Fischer, Tobias P.; Hilton, David R.

    2005-08-01

    The first historical eruption of Anatahan volcano began on May 10, 2003. Samples of tephra from early in the eruption were analyzed for major and trace elements, and Sr, Nd, Pb, Hf, and O isotopic compositions. The compositions of these tephras are compared with those of prehistoric samples of basalt and andesite, also newly reported here. The May 2003 eruptives are medium-K andesites with 59-63 wt.% SiO 2, and are otherwise homogeneous (varying less than 3% 2 σ about the mean for 45 elements). Small, but systematic, chemical differences exist between dark (scoria) and light (pumice) fragments, which indicate fewer mafic and oxide phenocrysts in, and less degassing for, the pumice than scoria. The May 2003 magmas are nearly identical to other prehistoric eruptives from Anatahan. Nonetheless, Anatahan has erupted a wide range of compositions in the past, from basalt to dacite (49-66 wt.% SiO 2). The large proportion of lavas with silicic compositions at Anatahan (> 59 wt.% SiO 2) is unique within the active Mariana Islands, which otherwise erupt a narrow range of basalts and basaltic andesites. The silicic compositions raise the question of whether they formed via crystal fractionation or crustal assimilation. The lack of 87Sr/ 86Sr variation with silica content, the MORB-like δ18O, and the incompatible behavior of Zr rule out assimilation of old crust, altered crust, or zircon-saturated crustal melts, respectively. Instead, the constancy of isotopic and trace element ratios, and the systematic variations in REE patterns are consistent with evolution by crystal fractionation of similar parental magmas. Thus, Anatahan is a type example of an island-arc volcano that erupts comagmatic basalts to dacites, with no evidence for crustal assimilation. The parental magmas to Anatahan lie at the low 143Nd/ 144Nd, Ba/La, and Sm/La end of the spectrum of magmas erupted in the Marianas arc, consistent with 1-3 wt.% addition of subducted sediment to the mantle source, or

  2. GPS Observations in Anatahan Island, Northern Mariana Islands, Before and After the Eruption of May 10, 2003

    NASA Astrophysics Data System (ADS)

    Kato, T.; Matsushima, T.; Nakada, S.; Watanabe, T.; Tabei, T.; Camacho, J. T.; Chong, R.

    2003-12-01

    A GPS observation project in the Northern Mariana Islands started in 1992 by a joint team of Japan, US and Saipan, CNMI. We have repeated surveys in the area for several times until 2003. Previous results until 1999 showed clear evidence of back arc spreading at the Mariana Trough with velocities of 4-6cm/yr with respect to the Philippine Sea plate. There was no significant volcanic activity in this time periods. Anatahan Island erupted about three months after the latest resurvey of January 2003. In order to investigate crustal deformations of the island related to this eruption, we established a permanent site in Anatahan (ANAT). The site is located about 7km WNW of the active crater in the eastern caldera. Solar panel and a lead battery are used for power and a small PC is used for automatic data archive. A special stainless pillar was designed to set up right above the pre-occupied benchmark. We also established a new permanent site in Saipan on the roof of EMO (EMO1). For a tie to the old site in Saipan, we conducted a survey at MPLC site that have been repeatedly occupied. For a preliminary analysis, we used data sets of 1994, 1998, 1999 and January and July of 2003 for a baseline MPLC - ANAT. The obtained results suggest that Anatahan has linearly drifted to northwest relative to Saipan at least until January 2003 with a rate of about 6.5mm/yr. There was no clear long-term vertical motion of Anatahan Island until January 2003. Comparison of position of ANAT relative to MPLC between January and July, 2003 suggests about 19cm subsidence of the island. Horizontal displacement may not be significant though a few centimeters of offset toward north could be visible. Large subsidence may be due to the spouting of magma. In order to monitor the crustal deformation during the volcanic activity, we are continuing GPS tracking at the established two permanent sites for a while.

  3. Stable isotope compositions of serpentinite seamounts in the Mariana forearc: Serpentinization processes, fluid sources and sulfur metasomatism

    USGS Publications Warehouse

    Alt, J.C.; Shanks, Wayne C.

    2006-01-01

    The Mariana and Izu-Bonin arcs in the western Pacific are characterized by serpentinite seamounts in the forearc that provide unique windows into the mantle wedge. We present stable isotope (O, H, S, and C) data for serpentinites from Conical seamount in the Mariana forearc and S isotope data for Torishima seamount in the Izu-Bonin forearc in order to understand the compositions of fluids and temperatures of serpentinization in the mantle wedge, and to investigate the transport of sulfur from the slab to the mantle wedge. Six serpentine mineral separates have a restricted range of ??18O (6.5-8.5???). Antigorite separates have ??D values of -29.5??? to -45.5??? that reflect serpentinization within the mantle wedge whereas chrysotile has low ??D values (-51.8??? to -84.0???) as the result of re-equilibration with fluids at low temperatures. Fractionation of oxygen isotopes between serpentine and magnetite indicate serpentinization temperatures of 300-375 ??C. Two late cross-fiber chrysotile veins have higher ??18O values of 8.9??? to 10.8??? and formed at lower temperatures (as low as ???100 ??C). Aqueous fluids in equilibrium with serpentine at 300-375 ??C had ??18O = 6.5-9??? and ??D = -4??? to -26???, consistent with sediment dehydration reactions at temperatures <200 ??C in the subducting slab rather than a basaltic slab source. Three aragonite veins in metabasalt and siltstone clasts within the serpentinite flows have ??18O = 16.7-24.5???, consistent with the serpentinizing fluids at temperatures <250 ??C. ??13C values of 0.1-2.5??? suggest a source in subducting carbonate sediments. The ??34S values of sulfide in serpentinites on Conical Seamount (-6.7??? to 9.8???) result from metasomatism through variable reduction of aqueous sulfate (??34S = 14???) derived from slab sediments. Despite sulfur metasomatism, serpentinites have low sulfur contents (generally < 164 ppm) that reflect the highly depleted nature of the mantle wedge. The serpentinites are mostly

  4. Ultramafic clasts from the South Chamorro serpentine mud volcano reveal a polyphase serpentinization history of the Mariana forearc mantle

    NASA Astrophysics Data System (ADS)

    Kahl, Wolf-Achim; Jöns, Niels; Bach, Wolfgang; Klein, Frieder; Alt, Jeffrey C.

    2015-06-01

    Serpentine seamounts located on the outer half of the pervasively fractured Mariana forearc provide an excellent window into the forearc devolatilization processes, which can strongly influence the cycling of volatiles and trace elements in subduction zones. Serpentinized ultramafic clasts recovered from an active mud volcano in the Mariana forearc reveal microstructures, mineral assemblages and compositions that are indicative of a complex polyphase alteration history. Petrologic phase relations and oxygen isotopes suggest that ultramafic clasts were serpentinized at temperatures below 200 °C. Several successive serpentinization events represented by different vein generations with distinct trace element contents can be recognized. Measured in situ Rb/Cs ratios are fairly uniform ranging between 1 and 10, which is consistent with Cs mobilization from sediments at lower temperatures and lends further credence to the low-temperature conditions proposed in models of the thermal structure in forearc settings. Late veins show lower fluid mobile element (FME) concentrations than early veins, suggesting a decreasing influence of fluid discharge from the subducting slab on the composition of the serpentinizing fluids. The continuous microfabric and mineral chemical evolution observed in the ultramafic clasts may have implications as to the origin and nature of the serpentinizing fluids. We hypothesize that opal and smectite dehydration produce quartz-saturated fluids with high FME contents and Rb/Cs between 1 and 4 that cause the early pervasive serpentinization. The partially serpentinized material may then be eroded from the basal plane of the suprasubduction mantle wedge. Serpentinization continued but the interacting fluids did not carry a pronounced sedimentary signature, either because FMEs were no longer released from the slab, or due to an en route loss of FMEs. Late chrysotile veins that document the increased access of fluids in a now fluid-dominated regime are

  5. Colwellia marinimaniae sp. nov., a hyperpiezophilic species isolated from an amphipod within the Challenger Deep, Mariana Trench.

    PubMed

    Kusube, Masataka; Kyaw, Than S; Tanikawa, Kumiko; Chastain, Roger A; Hardy, Kevin; Cameron, James; Bartlett, Douglas H

    2016-11-16

    An obligately piezophilic strain was isolated from an amphipod crustacean obtained in the Challenger Deep region of the Mariana Trench during the DEEPSEA CHALLENGE expedition. The strain, MTCD1T, grew at extremely high hydrostatic pressures, with a growth range of 80-140 MPa (optimum: 120 MPa) at 6°C. Phylogenetic analyses based on the 16S rRNA gene sequence indicates that it is closely affiliated with the genus Colwellia. Comparative 16S rRNA gene sequence analyses revealed 95.7%, 95.5%, and 95.2% similarity to Colwellia maris ABE-1T, Colwellia piezophila Y233GT, and Colwellia psychrerythraea 34HT, respectively. The major cellular fatty acids were C16:1, C16:0 and C22:6 (DHA) and the sole isoprenoid quinone produced was ubiqinone-8. DNA G+C content was 48.6 mol%. The strain was positive for oxidase and catalase activities. Based on the results from this study, strain MTCD1T is a novel Gram-negative species of the genus Colwellia, and the name Colwellia marinimaniae sp. nov. (type strain MTCD1T = ATCC TSD-5T = JCM 30270T) is proposed. It is the most piezophilic organism yet described.

  6. Microbial communities in iron-silica-rich microbial mats at deep-sea hydrothermal fields of the Southern Mariana Trough.

    PubMed

    Kato, Shingo; Kobayashi, Chiyori; Kakegawa, Takeshi; Yamagishi, Akihiko

    2009-08-01

    The abundance, diversity and composition of bacterial and archaeal communities in the microbial mats at deep-sea hydrothermal fields were investigated, using culture-independent 16S rRNA and functional gene analyses combined with mineralogical analysis. Microbial mats were collected at two hydrothermal areas on the ridge of the back-arc spreading centre in the Southern Mariana Trough. Scanning electron microscope and energy dispersive X-ray spectroscopic (SEM-EDS) analyses revealed that the mats were mainly composed of amorphous silica and contained numerous filamentous structures of iron hydroxides. Direct cell counting with SYBR Green I staining showed that the prokaryotic cell densities were more than 10(8) cells g(-1). Quantitative polymerase chain reaction (Q-PCR) analysis revealed that Bacteria are more abundant than Archaea in the microbial communities. Furthermore, zetaproteobacterial cells accounted for 6% and 22% of the prokaryotic cells in each mat estimated by Q-PCR with newly designed primers and TaqMan probe. Phylotypes related to iron-oxidizers, methanotrophs/methylotrophs, ammonia-oxidizers and sulfate-reducers were found in the 16S rRNA gene clone libraries constructed from each mat sample. A variety of unique archaeal 16S rRNA gene phylotypes, several pmoA, dsrAB and archaeal amoA gene phylotypes were also recovered from the microbial mats. Our results provide insights into the diversity and abundance of microbial communities within microbial mats in deep-sea hydrothermal fields.

  7. An exploration for hydrothermal plume evolution using the AUV "URASHIMA" with fluid sampling system at southern Mariana Trough

    NASA Astrophysics Data System (ADS)

    Noguchi, T.; Sunamura, M.; Yamamoto, H.; Fukuba, T.; Okino, K.; Sugiyama, T.; Okamura, K.

    2009-12-01

    Hydrothermal fluids contain high concentration of anoxic chemical species, i.e. methane and hydrogen sulfide, helium-3, and heavy metals derived from the rock-water interaction. During the hydothermal plume spreading, it is known that several chemical species are oxidized which include available energy source for microorganism, however, few results have been reported on the spatial variation of both of chemical and microbiological concentration and species. In the southern Mariana Trough, some site surveys have been conducted with CTD hydrocasts, the manned submersible, and ROVs since 2003. In this field, three hydrothermal vent sites were discovered within the small area, where the chemistry of each hydrothermal fluid was different from each other. These differences of chemistry are prospected to affect the individual plume evolution. In order to discuss the each hydrothermal plume evolution, we conducted high-resolution plume mapping by the AUV "URASHIMA" with some chemical sensors. Additionally, we loaded 24 bottles of water sampler for the geochemical and microbial analysis. During this cruise, we detected hydrothermal plume anomalies derived from each hydrothermal site with the highly precise topographic results. Based on the results, we will discuss the relationships between the spreading of hydrothermal plume (geochemical evolution) and the ecology of plume microbes.

  8. Distribution and origin of igneous rocks from the landward slopes of the Mariana Trench: Implications for its structure and evolution

    SciTech Connect

    Bloomer, S.H.

    1983-09-10

    The landward slope of the Mariana Trench is composed largely of igneous rocks. Serpentinites and serpentinized ultramafic rocks occur at nearly all structural levels on the slope from depths of 8000 to 1200 m. Seamountlike features on the trench slope break are the surface expression of serpentinite diapirs. Cumulate and massive gabbros are found; several varieties of volcanic rocks are common including boninites, altered and metamorphosed basalts, andesites, and dacites. The chemical characteristics of the volcanic rocks indicate that nearly all are products of island arc volcanism. Together with the gabbros, these volcanic rocks represent what is probably a late Eocene arc complex. These rocks were probably the first volcanic products to result from the subduction of the Pacific plate beneath the Phillippine Sea plate; their exposure on the trench slope today implies a significant amount of tectonic erosion of the landward slope since Eocene time. Most of this removal of material appears to have occurred during the early stages of subduction. There are isolated occurrences on the landward slope of rock assemblages including alkalic basalts, chert, hyaloclastites, upper Cretaceous siliceous sediments, and shallow water limestones. These assemblages are very similar to rocks dredged from seamounts on the offshore flank of the trench, and their presence on the landward slope suggests that since the cessation of vigorous tectonic erosion, there has been episodic accretion of seamount fragments to the landward slope.

  9. Abundance of Zetaproteobacteria within crustal fluids in back-arc hydrothermal fields of the Southern Mariana Trough.

    PubMed

    Kato, Shingo; Yanagawa, Katsunori; Sunamura, Michinari; Takano, Yoshinori; Ishibashi, Jun-ichiro; Kakegawa, Takeshi; Utsumi, Motoo; Yamanaka, Toshiro; Toki, Tomohiro; Noguchi, Takuroh; Kobayashi, Kensei; Moroi, Arimichi; Kimura, Hiroyuki; Kawarabayasi, Yutaka; Marumo, Katsumi; Urabe, Tetsuro; Yamagishi, Akihiko

    2009-12-01

    To extend knowledge of subseafloor microbial communities within the oceanic crust, the abundance, diversity and composition of microbial communities in crustal fluids at back-arc hydrothermal fields of the Southern Mariana Trough (SMT) were investigated using culture-independent molecular techniques based on 16S rRNA gene sequences. Seafloor drilling was carried out at two hydrothermal fields, on- and off-ridge of the back-arc spreading centre of the SMT. 16S rRNA gene clone libraries for bacterial and archaeal communities were constructed from the fluid samples collected from the boreholes. Phylotypes related to Thiomicrospira in the Gammaproteobacteria (putative sulfide-oxidizers) and Mariprofundus in the Zetaproteobacteria (putative iron-oxidizers) were recovered from the fluid samples. A number of unique archaeal phylotypes were also recovered. Fluorescence in situ hybridization (FISH) analysis indicated the presence of active bacterial and archaeal populations in the fluids. The Zetaproteobacteria accounted for up to 32% of the total prokaryotic cell number as shown by FISH analysis using a specific probe designed in this study. Our results lead to the hypothesis that the Zetaproteobacteria play a role in iron oxidation within the oceanic crust.

  10. Fe3+/∑Fe variation in Mariana arc and back-arc magmas and primary fO2 of the mantle wedge

    NASA Astrophysics Data System (ADS)

    Brounce, M. N.; Kelley, K. A.; Cottrell, E.

    2012-12-01

    Arc basalts are more oxidized than mid-ocean ridge basalts (MORB), but this could be due to differentiation processes in the Earth's crust or to a fundamental difference in the oxygen fugacity (fO2) in their respective mantle sources. To test between these two hypotheses, we present major element, S and Cl concentrations (EMP), trace element concentrations (LA-ICPMS), volatile concentrations (FTIR) and Fe3+/ΣFe ratios (μ-XANES) determined from naturally glassy olivine-hosted melt inclusions with <2% post-entrapment crystallization from single eruptive events at Sarigan, Alamagan, Agrigan, Guguan, Pagan, and Maug volcanoes, and submarine glasses from Pagan and NW Rota-1 volcanoes and the Mariana Trough back-arc spreading center. Near-undifferentiated glass compositions from Pagan and NW Rota-1 submarine volcanoes have high MgO (7.9 - 11.1 wt%) and Fe3+/ΣFe ratios that are significantly oxidized over MORB, ranging from 0.211 to 0.237. Melt inclusions and Mariana Trough glasses range from 2.0 - 7.4 wt% MgO and are consistent with variable quantities of olivine ± clinopyroxene ± plagioclase ± magnetite fractionation as well as CO2 ± H2O ± S degassing. Fe3+/ΣFe ratios in arc melt inclusions range from 0.18 - 0.34 and in back-arc related samples from 0.17 - 0.20. Variations in Fe3+/ΣFe ratio along observed liquid lines of descent (LLDs) are slightly oxidizing, consistent with the fractionation of Fe2+-bearing minerals, or moderately reducing when either sulfur is behaving as a volatile or when magnetite is fractionating, but are insufficient in magnitude to explain why arc basalts are more oxidized than MORB. Melts last in equilibrium with the mantle (i.e. primary melts) are reconstructed from measured compositions by backtracking along empirical and modeled LLDs to yield primary Fe3+/ΣFe ratios. Pressures and temperatures of primary melt equilibration are calculated using the Si-thermobarometer developed by Lee et al. [2009] (EPSL 279(1-2), 20-33). Primary

  11. Bait attending fishes of the abyssal zone and hadal boundary: Community structure, functional groups and species distribution in the Kermadec, New Hebrides and Mariana trenches

    NASA Astrophysics Data System (ADS)

    Linley, T. D.; Stewart, A. L.; McMillan, P. J.; Clark, M. R.; Gerringer, M. E.; Drazen, J. C.; Fujii, T.; Jamieson, A. J.

    2017-03-01

    Baited landers were deployed at 83 stations at four locations in the west Pacific Ocean from bathyal to hadal depths: The Kermadec Trench, the New Hebrides Trench, the adjoining South Fiji Basin and the Mariana Trench. Forty-seven putative fish species were observed. Distinct fish faunal groups were identified based on maximum numbers and percentage of observations. Both analyses broadly agreed on the community structure: A bathyal group at <3000 m in the New Hebrides and Kermadec trenches, an abyssal group (3039 - 4692 m) in the Kermadec Trench, an abyssal-hadal transition zone (AHTZ) group (Kermadec: 4707-6068 m, Mariana: 4506-6198 m, New Hebrides: 2578-6898 m, South Fiji Basin: 4074-4101 m), and a hadal group of endemic snailfish in the Kermadec and Mariana trenches (6750-7669 m and 6831-8143 m respectively). The abyssal and hadal groups were absent from the New Hebrides Trench. Depth was the single factor that best explained the biological variation between samples (16%), the addition of temperature and average surface primary production for the previous year increased this to 36% of variation. The absence of the abyssal group from the New Hebrides Trench and South Fiji Basin was due to the absence of macrourids (Coryphaenoides spp.), which defined the group. The macrourids may be energetically limited in these areas. In their absence the species of the AHTZ group appear released of competition with the macrourids and are found far shallower at these sites. The fish groups had distinct feeding strategies while attending the bait: The bathyal and abyssal groups were almost exclusively necrophagous, the AHTZ group comprised predatory and generalist feeders, while the hadal snailfishes were exclusively predators. With increasing depth, predation was found to increase while scavenging decreased. The data suggest scavenging fish fauna do not extend deeper than the hadal boundary.

  12. Collecting, Preserving & Sharing Information in Micronesia. Proceedings of the Annual Pacific Islands Association of Libraries and Archives Conference (3rd, Saipan, Northern Mariana Islands, October 13-15, 1993).

    ERIC Educational Resources Information Center

    Pacific Islands Association of Libraries and Archives, Guam.

    Participants from Washington, Hawaii, Majuro, Palau, Guam and other points in the Northern Mariana Islands came together to share information relating to the functions of libraries and archives as information banks and as preservers of the cultural heritage of Micronesia. Papers presented were: (1) "Reading Motivation in the Pacific"…

  13. Changes in polyamines, inorganic ions and glutamine synthetase activity in response to nitrogen availability and form in red spruce (Picea rubens).

    PubMed

    Serapiglia, Michelle J; Minocha, Rakesh; Minocha, Subhash C

    2008-12-01

    We analyzed effects of nitrogen availability and form on growth rates, concentrations of polyamines and inorganic ions and glutamine synthetase activity in in-vitro-cultured red spruce (Picea rubens Sarg.) cells. Growth rates, concentrations of polyamines and glutamine synthetase activity declined when either the amount of nitrate or the total amount of N in the culture medium was reduced. When total N in the medium was increased, cell mass increased without significant changes in glutamine synthetase activity or polyamine concentration. Reductions in the amount of nitrate or total N in the culture medium resulted in increased accumulations of Ca, Mn and Zn in the cells, and K accumulation decreased in response to decreasing nitrate:ammonium ratios. The data indicate that changes in total N availability as well as the forms of N play important roles in the physiological responses of in-vitro-grown red spruce cells that mimic the observed responses of forest trees to soil N deficiency and N fertilization.

  14. [Spatial Distribution of Intron 2 of nad1 Gene Haplotypes in Populations of Norway and Siberian Spruce (Picea abies-P. obovata) Species Complex].

    PubMed

    Mudrik, E A; Polyakova, T A; Shatokhina, A V; Bondarenko, G N; Politov, D V

    2015-10-01

    The length and sequence variations among intron 2 haplotypes of the mitochondrial DNA nad1 gene have been studied in the Norway and Siberian spruce (Picea abies (L.) H. Karst.-P. obovata Ledeb.) species complex. Twenty-two native populations and 15 provenances were analyzed. The distribution of the northern European haplogroup (haplotypes 721, 755, 789, 823, 857, 891, and 925) is delimited in the west by the Ural region inclusively. Haplotype 712 is widespread in populations of Siberia, in the Far East and in northeastern Russia. A novel variant of the Siberian haplogroup (780) containing three copies of the first minisatellite motif (34 bp) was found for the first time. The absence of an admixture of the northern European and Siberian haplotypes in the zone of spruce species introgression previously marked by morphological traits and nuclear allozyme loci was demonstrated. This may be evidence of the existence of a sharper geographic boundary between the two haplogroups, as compared to a boundary based on phenotypic and allozyme data. A high proportion of the interpopulation component of variation (65%) estimated by AMOVA indicates a substantial genetic subdivision of European and Siberian populations of the Palearctic spruce complex by mtDNA, which can be putatively explained by natural barriers to gene flow with seeds related, for instance, to the woodless regions of the western Siberian Plain in the Pleistocene and the probable floodplains of large rivers.

  15. The impact of long-term CO2 enrichment on sun and shade needles of Norway spruce (Picea abies): photosynthetic performance, needle anatomy and phenolics accumulation.

    PubMed

    Lhotáková, Zuzana; Urban, Otmar; Dubánková, Marianna; Cvikrová, Milena; Tomášková, Ivana; Kubínová, Lucie; Zvára, Karel; Marek, Michal V; Albrechtová, Jana

    2012-06-01

    Norway spruce (Picea abies L. Karst) grown under ambient (365-377 μmol(CO(2)) mol(-1); AC) and elevated (700 μmol(CO(2)) mol(-1); EC) CO(2) concentrations within glass domes with automatically adjustable windows and on an open-air control site were studied after 8 years of treatment. The effect of EC on photosynthesis, mesophyll structure and phenolics accumulation in sun and shade needles was examined. Photosynthetic assimilation and dark respiration rates were measured gasometrically; the structural parameters of mesophyll were determined using confocal microscopy and stereological methods. The contents of total soluble phenolics and lignin were assessed spectrophotometrically, and localizations of different phenolic groups were detected histochemically on needle cross-sections. EC enhanced the light-saturated CO(2) assimilation rate and reduced dark respiration in the current-year needles. No effects of CO(2) enrichment on mesophyll structural parameters were observed. Similarly, the accumulation and localization of phenolics and lignin remained unaffected by EC treatment. Needles differentiated into sun and shade ecotypes in the same manner and to the same extent irrespective of CO(2) treatment. Based on these results, it is apparent that the EC-induced enhancement of photosynthesis is not related to changes in the examined structural parameters of mesophyll and accumulation of phenolic compounds.

  16. Bio-Guided Isolation of Methanol-Soluble Metabolites of Common Spruce (Picea abies) Bark by-Products and Investigation of Their Dermo-Cosmetic Properties.

    PubMed

    Angelis, Apostolis; Hubert, Jane; Aligiannis, Nektarios; Michalea, Rozalia; Abedini, Amin; Nuzillard, Jean-Marc; Gangloff, Sophie C; Skaltsounis, Alexios-Leandros; Renault, Jean-Hugues

    2016-11-21

    Common spruce (Picea abies L.) is a fast-growing coniferous tree, widely used in several countries for the production of sawn wood, timber and pulp. During this industrial exploitation, large quantities of barks are generated as waste materials. The aim of this study was the bio-guided investigation and the effective recovery of methanol-soluble metabolites of common spruce bark for the development of new dermo-cosmetic agents. The active methanol extract was initially fractionated by Centrifugal Partition Chromatography (CPC) using a triphasic solvent system in a step-gradient elution mode. All resulting fractions were evaluated for their antibacterial activity, antioxidant activity and their capability to inhibit tyrosinase, elastase and collagenase activity. In parallel, the chemical composition of each fraction was established by combining a (13)C-NMR dereplication approach and 2D-NMR analyses. As a result, fourteen secondary metabolites corresponding to stilbene, flavonoid and phenolic acid derivatives were directly identified in the CPC fractions. A high amount (0.93 g) of E-astringin was recovered from 3 g of crude extract in a single 125 min run. E-Astringin significantly induced the tyrosinase activity while E-piceid, taxifolin, and taxifolin-3'-O-glucopyranoside exhibited significant anti-tyrosinase activity. The above compounds showed important anti-collagenase and antimicrobial activities, thus providing new perspectives for potential applications as cosmetic ingredients.

  17. Genetic structure of Sakhalin spruce (Picea glehnii) in northern Japan and adjacent regions revealed by nuclear microsatellites and mitochondrial gene sequences.

    PubMed

    Aizawa, Mineaki; Yoshimaru, Hiroshi; Takahashi, Makoto; Kawahara, Takayuki; Sugita, Hisashi; Saito, Hideyuki; Sabirov, Renat N

    2015-01-01

    The genetic structure of Sakhalin spruce (Picea glehnii) was studied across the natural range of the species, including two small isolated populations in south Sakhalin and Hayachine, by using six microsatellite loci and maternally inherited mitochondrial gene sequences. We also analyzed P. jezoensis, a sympatric spruce in the range. Genetic diversity of P. glehnii was higher in central Hokkaido and the lowest in the Hayachine. Bayesian clustering and principal coordinate analysis by using the microsatellites indicated that the Hayachine was clearly distinct from other populations, implying that it had undergone strong genetic drift since the last glacial period. P. glehnii harbored four mitochondrial haplotypes, two of which were shared with P. jezoensis. One of the two was observed without geographical concentration, suggesting its derivation from ancestral polymorphism. Another was observed in south Sakhalin and in P. jezoensis across Sakhalin. The Bayesian clustering--by using four microsatellite loci, including P. jezoensis populations--indicated unambiguous species delimitation, but with possible admixture of P. jezoensis genes into P. glehnii in south Sakhalin, where P. glehnii is abundantly overwhelmed by P. jezoensis; this might explain the occurrence of introgression of the haplotype of P. jezoensis into P. glehnii.

  18. Induction of isoprenyl diphosphate synthases, plant hormones and defense signalling genes correlates with traumatic resin duct formation in Norway spruce (Picea abies).

    PubMed

    Schmidt, Axel; Nagel, Raimund; Krekling, Trygve; Christiansen, Erik; Gershenzon, Jonathan; Krokene, Paal

    2011-12-01

    Norway spruce (Picea abies) defends itself against herbivores and pathogens by formation of traumatic resin ducts filled with terpenoid-based oleoresin. An important group of enzymes in terpenoid biosynthesis are the short-chain isoprenyl diphosphate synthases which produce geranyl diphosphate (C(10)), farnesyl diphosphate (C(15)), and geranylgeranyl diphosphate (C(20)) as precursors of monoterpenes, sesquiterpenes, and diterpene resin acids, respectively. After treatment with methyl jasmonate (MJ) we investigated the expression of all isoprenyl diphosphate synthase genes characterized to date from Norway spruce and correlated this with formation of traumatic resin ducts and terpene accumulation. Formation of traumatic resin ducts correlated with higher amounts of monoterpenes, sesquiterpenes and diterpene resin acids and an upregulation of isoprenyl diphosphate synthase genes producing geranyl diphosphate or geranylgeranyl diphosphate. Among defense hormones, jasmonate and jasmonate-isoleucine conjugate accumulated to higher levels in trees with extensive traumatic resin duct formation, whereas salicylate did not. Jasmonate and ethylene are likely to both be involved in formation of traumatic resin ducts based on elevated transcripts of genes encoding lipoxygenase and 1-aminocyclopropane-1-carboxylic acid oxidase associated with resin duct formation. Other genes involved in defense signalling in other systems, mitogen-activated protein kinase3 and nonexpressor of pathogenesis-related gene1, were also associated with traumatic resin duct formation. These responses were detected not only at the site of MJ treatment, but also systemically up to 60 cm above the site of treatment on the trunk.

  19. Molecular control of normal and acrocona mutant seed cone development in Norway spruce (Picea abies) and the evolution of conifer ovule-bearing organs.

    PubMed

    Carlsbecker, Annelie; Sundström, Jens F; Englund, Marie; Uddenberg, Daniel; Izquierdo, Liz; Kvarnheden, Anders; Vergara-Silva, Francisco; Engström, Peter

    2013-10-01

    Reproductive organs in seed plants are morphologically divergent and their evolutionary history is often unclear. The mechanisms controlling their development have been extensively studied in angiosperms but are poorly understood in conifers and other gymnosperms. Here, we address the molecular control of seed cone development in Norway spruce, Picea abies. We present expression analyses of five novel MADS-box genes in comparison with previously identified MADS and LEAFY genes at distinct developmental stages. In addition, we have characterized the homeotic transformation from vegetative shoot to female cone and associated changes in regulatory gene expression patterns occurring in the acrocona mutant. The analyses identified genes active at the onset of ovuliferous and ovule development and identified expression patterns marking distinct domains of the ovuliferous scale. The reproductive transformation in acrocona involves the activation of all tested genes normally active in early cone development, except for an AGAMOUS-LIKE6/SEPALLATA (AGL6/SEP) homologue. This absence may be functionally associated with the nondeterminate development of the acrocona ovule-bearing scales. Our morphological and gene expression analyses give support to the hypothesis that the modern cone is a complex structure, and the ovuliferous scale the result of reductions and compactions of an ovule-bearing axillary short shoot in cones of Paleozoic conifers.

  20. Profiling functions of ectomycorrhizal diversity and root structuring in seedlings of Norway spruce (Picea abies) with fast- and slow-growing phenotypes.

    PubMed

    Velmala, Sannakajsa M; Rajala, Tiina; Heinonsalo, Jussi; Taylor, Andy F S; Pennanen, Taina

    2014-01-01

    We studied the role of taxonomical and functional ectomycorrhizal (ECM) fungal diversity in root formation and nutrient uptake by Norway spruce (Picea abies) seedlings with fast- and slow-growing phenotypes. Seedlings were grown with an increasing ECM fungal diversity gradient from one to four species and sampled before aboveground growth differences between the two phenotypes were apparent. ECM fungal colonization patterns were determined and functional diversity was assayed via measurements of potential enzyme activities of eight exoenzymes probably involved in nutrient mobilization. Phenotypes did not vary in their receptiveness to different ECM fungal species. However, seedlings of slow-growing phenotypes had higher fine-root density and thus more condensed root systems than fast-growing seedlings, but the potential enzyme activities of ectomycorrhizas did not differ qualitatively or quantitatively. ECM species richness increased host nutrient acquisition potential by diversifying the exoenzyme palette. Needle nitrogen content correlated positively with high chitinase activity of ectomycorrhizas. Rather than fast- and slow-growing phenotypes exhibiting differing receptiveness to ECM fungi, our results suggest that distinctions in fine-root structuring and in the belowground growth strategy already apparent at early stages of seedling development may explain later growth differences between fast- and slow-growing families.

  1. Updating beliefs and combining evidence in adaptive forest management under climate change: a case study of Norway spruce (Picea abies L. Karst) in the Black Forest, Germany.

    PubMed

    Yousefpour, Rasoul; Temperli, Christian; Bugmann, Harald; Elkin, Che; Hanewinkel, Marc; Meilby, Henrik; Jacobsen, Jette Bredahl; Thorsen, Bo Jellesmark

    2013-06-15

    We study climate uncertainty and how managers' beliefs about climate change develop and influence their decisions. We develop an approach for updating knowledge and beliefs based on the observation of forest and climate variables and illustrate its application for the adaptive management of an even-aged Norway spruce (Picea abies L. Karst) forest in the Black Forest, Germany. We simulated forest development under a range of climate change scenarios and forest management alternatives. Our analysis used Bayesian updating and Dempster's rule of combination to simulate how observations of climate and forest variables may influence a decision maker's beliefs about climate development and thereby management decisions. While forest managers may be inclined to rely on observed forest variables to infer climate change and impacts, we found that observation of climate state, e.g. temperature or precipitation is superior for updating beliefs and supporting decision-making. However, with little conflict among information sources, the strongest evidence would be offered by a combination of at least two informative variables, e.g., temperature and precipitation. The success of adaptive forest management depends on when managers switch to forward-looking management schemes. Thus, robust climate adaptation policies may depend crucially on a better understanding of what factors influence managers' belief in climate change.

  2. Bisphenol A affects germination and tube growth in Picea meyeri pollen through modulating Ca2+ flux and disturbing actin-dependent vesicular trafficking during cell wall construction.

    PubMed

    Chang, Tongjie; Fan, Chengyu; Man, Yi; Zhou, Junhui; Jing, Yanping

    2015-09-01

    Bisphenol A (BPA), a widespread pollutant, is reportedly harmful to humans, animals and plants. However, the effect of BPA on plant pollen tube growth, as well as the mechanism involved, remains unclear. Here, we report that BPA significantly inhibited Picea meyeri pollen germination and tube elongation in a dose-dependent manner. Transmission electron microscopy showed that BPA was detrimental to organelles such as mitochondria and Golgi apparatus. Non-invasive detection revealed that BPA inhibited extracellular Ca(2+) influx and promoted intracellular Ca(2+) efflux at the pollen tube tip, thereby inducing a dissipated Ca(2+) gradient. Fluorescence labeling showed that BPA disorganized actin filaments (AFs), which subsequently led to abnormal vesicle trafficking. Furthermore, BPA reduced the activity of acid phosphatase, a typical exocytosis enzyme. Moreover, Fourier transform infrared (FTIR) analysis and subsequent fluorescence labeling revealed that BPA induced an abnormal deposition of cell wall components, including pectins and callose. Taken together, our results indicate that BPA, a ubiquitous environmental pollutant, disturbs Ca(2+) flux in P. meyeri pollen tubes, thus disrupting AF organization, resulting in abnormal actin-dependent vesicle trafficking and further affecting the deposition of cell wall components. These findings provide new insight into the mechanism of BPA toxicity in pollen tube tip growth.

  3. Variations in stable carbon isotope composition and leaf traits of Picea schrenkiana var. tianschanica along an altitude gradient in Tianshan Mountains, northwest China.

    PubMed

    Zhang, Huiwen; Ma, Jianying; Sun, Wei; Chen, Fahu

    2014-01-01

    To understand the morphological and physiological responses of leaves to changes in altitudinal gradients, we examined ten morphological and physiological characteristics in one-year-old needles of Picea schrenkiana var. tianschanica at ten points along an altitudinal gradient from 1420 to 2300 m a.s.l. on the northern slopes of the Tianshan Mountains in northwest China. Our results indicated that LA, SD, LPC, and LKC increased linearly with increasing elevation, whereas leaf δ13C, LNC, Chla+b, LDMC, LMA, and Narea varied nonlinearly with changes in altitude. With elevation below 2100 m, LNC, Narea, and Chla+b increased, while LDMC and LMA decreased with increasing altitude. When altitude was above 2100 m, these properties showed the opposite patterns. Leaf δ13C was positively correlated with Narea and LNC and negatively correlated with SD and LA, suggesting that leaf δ13C was indirectly controlled by physiological and morphological adjustments along altitudinal gradients. Based on the observed maximum values in LNC, Narea, Chla+b, and LA and the minimum values in LMA and LDMC at the elevation of 2100 m, suggesting higher photosynthetic capacity and greater potential for fast growth under superior optimum zone, we concluded that the best growing elevation for P. schrenkiana var. tianschanica in the Tianshan Mountains was approximately 2100 m.

  4. Age-dependent climate-growth relationships and regeneration of Picea abies in a drought-prone mixed coniferous forest in the Alps

    PubMed Central

    Schuster, Roman; Oberhuber, Walter

    2013-01-01

    Within dry inner Alpine environments climate warming is expected to affect the development of forest ecosystems by changing species composition and inducing shifts in forest distribution. By applying dendroecological techniques we evaluated climate sensitivity of radial growth and establishment of Picea abies in a drought-prone mixed-coniferous forest in the Austrian Alps. Time series of annual increments were developed from > 220 trees and assigned to four age classes. While radial growth of old P. abies trees (mean age 121 and 174 yr) responded highly significant to May-June precipitation, young trees (mean age 28 and 53 yr) were insensitive to precipitation in the current year. Because tree age was closely correlated to height and diameter (r2 = 0.709 and 0.784, respectively), we relate our findings to the increase in tree size rather than age per se. Synchronicity found among trend in basal area increment and tree establishment suggests that canopy openings increased light and water availability, which favoured growth and establishment of moderately shade-tolerant P. abies. We conclude that although P. abies is able to regenerate at this drought prone site, increasing inter-tree competition for water in dense stands gradually lowers competitive strength and restricts scattered occurrence to dry-mesic sites. PMID:24027351

  5. [Effects of forest gap on seasonal dynamics of soil organic carbon and microbial biomass car- bon in Picea asperata forest in Miyaluo of Western Sichuan, Southwest China].

    PubMed

    Zhou, Yi-Gui; Hao, Kai-Jie; Li, Xian-Wei; Fan, Chuan; Chen, Yue-Lin; Liu, Yun-Ke; Wang, Xie

    2014-09-01

    Effects of gap sizes (50, 100 and 150 m2) on seasonal dynamics of soil organic carbon (SOC) and microbial biomass carbon (MBC) in soil surface (0-15 cm) and subsurface (15-30 cm) were investigated in a 50-year old Picea asperata plantation in Miyaluo forest, Western Si- chuan, China. In the four seasons, the SOC and MBC contents were higher in the soil surface than in soil subsurface in the four treatments, and varied insignificantly in different seasons. Compared with the control, the SOC content increased by 35.4%, 21.2% and 10.3% in soil surface, and by 45.5%, 25.0% and 12.1% in soil subsurface with the forest gaps of 50, 100 and 150 m2, respectively. The MBC content increased by 26.7%, 16.7%, 11.3% and 24.4%, 12.6%, 7.3% in soil surface and subsurface, respectively. There were significant negative relationship between soil SOC content and the soil pH and moisture, and significant positive relationship between soil SOC content and soil temperature. Significant effects of SOC content, soil pH, soil temperature, soil moisture on MBC content were observed, suggesting that forest gaps could significantly affect envi- ronmental conditions, and increasing gaps could reduce microbial activity and decomposition rate and decrease soil SOC and MBC contents.

  6. Purification of Two Superoxide Dismutase Isozymes and Their Subcellular Localization in Needles and Roots of Norway Spruce (Picea abies L.) Trees 1

    PubMed Central

    Kröniger, Werner; Rennenberg, Heinz; Polle, Andrea

    1992-01-01

    Two isozymes of superoxide dismutase (SOD; EC 1.15.1.1) were purified from Norway spruce (Picea abies L.) needles to apparent electrophoretic homogeneity. Purification factors were 354 for SOD I and 265 for SOD II. The native molecular mass of both purified enzymes was approximately 33 kD, as determined by gel filtration. The subunit molecular weights, as estimated by sodium dodecyl sulfate polyacrylamide gel electrophoresis, were 20,000 for SOD I and 16,000 for SOD II in the presence of 2-mercaptoethanol, and 15,800 and 15,000, respectively, in its absence. These results indicate that the native enzymes were homodimers whose subunits contained intrachain disulfide bonds. Isoelectric points determined by nondenaturing isoelectric focusing were 4.5 and 5.5 for SOD I and II, respectively. NH2-terminal sequence analysis of the first 22 to 23 amino acids revealed 70 to 75% sequence identity with chloroplastic CuZn SODs from other plant species for SOD I, and 75% sequence identity with the cytosolic CuZn SOD from Scots pine for SOD II. SOD I was the major activity in needles and it was associated with chloroplasts. SOD II activity was dominant in roots. Images Figure 2 Figure 3 PMID:16652966

  7. Composition and Properties of Hydrogen Peroxide Decomposing Systems in Extracellular and Total Extracts from Needles of Norway Spruce (Picea abies L., Karst.) 1

    PubMed Central

    Polle, Andrea; Chakrabarti, Krisanu; Schürmann, Wolfgang; Renneberg, Heinz

    1990-01-01

    Hydrogen peroxide (H2O2) scavenging systems of spruce (Picea abies) needles were investigated in both extracts obtained from the extracellular space and extracts of total needles. As assessed by the lack of activity of symplastic marker enzymes, the extracellular washing fluid was free from intracellular contaminations. In the extracellular washing fluid ascorbate, glutathione, cysteine, and high specific activities of guaiacol peroxidases were observed. Guaiacol peroxidases in the extracellular washing fluid and needle homogenates had the same catalytic properties, i.e. temperature optimum at 50°C, pH optimum in the range of pH 5 to 6 and low affinity for guaiacol (apparent Km = 40 millimolar) and H2O2 (apparent Km = 1-3 millimolar). Needle homogenates contained ascorbate peroxidase, dehydroascorbate reductase, monodehydroascorbate reductase, glutathione reductase, and catalase, but not glutathione peroxidase activity. None of these activities was detected in the extracellular washing fluid. Ascorbate and glutathione related enzymes were freeze sensitive; ascorbate peroxidase was labile in the absence of ascorbate. The significance of extracellular antioxidants for the detoxification of injurious oxygen species is discussed. PMID:16667703

  8. Metabolite changes in conifer buds and needles during forced bud break in Norway spruce (Picea abies) and European silver fir (Abies alba)

    PubMed Central

    Dhuli, Priyanka; Rohloff, Jens; Strimbeck, G. Richard

    2014-01-01

    Environmental changes such as early spring and warm spells induce bud burst and photosynthetic processes in cold-acclimated coniferous trees and consequently, cellular metabolism in overwintering needles and buds. The purpose of the study was to examine metabolism in conifers under forced deacclimation (artificially induced spring) by exposing shoots of Picea abies (boreal species) and Abies alba (temperate species) to a greenhouse environment (22°C, 16/8 h D/N cycle) over a 9 weeks period. Each week, we scored bud opening and collected samples for GC/MS–based metabolite profiling. We detected a total of 169 assigned metabolites and 80 identified metabolites, comprising compounds such as mono- and disaccharides, Krebs cycle acids, amino acids, polyols, phenolics, and phosphorylated structures. Untargeted multivariate statistical analysis based on PCA and cluster analysis segregated samples by species, tissue type, and stage of tissue deacclimations. Similar patterns of metabolic regulation in both species were observed in buds (amino acids, Krebs cycle acids) and needles (hexoses, pentoses, and Krebs cycle acids). Based on correlation of bud opening score with compound levels, distinct metabolites could be associated with bud and shoot development, including amino acids, sugars, and acids with known osmolyte function, and secondary metabolites. This study has shed light on how elevated temperature affects metabolism in buds and needles of conifer species during the deacclimation phase, and contributes to the discussion about how phenological characters in conifers may respond to future global warming. PMID:25566281

  9. Effects of elevated carbon dioxide and drought on the growth and physiology of clonal Sitka spruce plants (Picea sitchensis (Bong.) Carr.).

    PubMed

    Townend, J

    1993-12-01

    Two-year-old Sitka spruce (Picea sitchensis (Bong.) Carr.) plants from four clones were grown in naturally lit growth chambers for 6 months at either ambient (350 ppm) or ambient + 250 ppm (600 ppm) CO(2) concentration. Plants were grown in large boxes filled with peat, in a system that allowed the roots of individual plants to be harvested easily at the end of the growing season. Half of the boxes were kept well watered and half were allowed to dry out slowly over the summer. Plants growing in elevated CO(2) showed a 6.9% increase in mean relative growth rate compared to controls in the drought treatment and a 9.8% increase compared to controls in the well-watered treatment, though there was considerable variation in response among the different clones and water treatments. Rates of net CO(2) assimilation were higher and stomatal conductances were lower in plants grown in elevated CO(2) than in ambient CO(2) in both the well-watered and drought treatments. Both of these factors contributed to the doubling of instantaneous water use efficiency. The partitioning of biomass to roots was unaffected by elevated CO(2), but the ratio of needle mass/stems + branches mass decreased. Together with reduced stomatal conductance, this probably caused the observed increases in xylem pressure potentials with elevated CO(2).

  10. Needle life span, photosynthetic rate and nutrient concentration of Picea glehnii, P. jezoensis and P. abies planted on serpentine soil in northern Japan.

    PubMed

    Kayama, Masazumi; Sasa, Kaichiro; Koike, Takayoshi

    2002-07-01

    We investigated the adaptation of three spruce species (Picea glehnii Masters, P. jezoensis Carr. and P. abies Karst.) to growth in northern Japan on serpentine soils (characterized by high concentrations of heavy metals and Mg, a low Ca/Mg ratio and low fertility) and fertile brown forest soils. Among species, seedling survival on serpentine soil was highest in P. glehnii. Shoot growth of P. glehnii was similar whether grown on serpentine or brown forest soil, whereas shoot growth of the other species was significantly less on serpentine soil than on brown forest soil. On serpentine soil, needle life span of P. glehnii was at least 3 years longer than that of the other two species. Needle area per shoot of P. glehnii was significantly higher on serpentine soil than on brown forest soil up to a shoot age of 8 years. In all three species, light-saturated photosynthetic rate (Pmax) decreased with needle age independently of soil type. However, on serpentine soil, Pmax in P. glehnii was higher, particularly in older needles, than in the other species. Furthermore, on serpentine soil, needle concentrations of nitrogen and phosphorus were higher in P. glehnii than in the other species. We conclude that P. glehnii is better adapted to serpentine soil than P. jezoensis and P. abies at least in part because of its greater needle life span and higher needle nutrient concentrations.

  11. Reconnaissance Study of the Hydrology of American Memorial Park, Island of Saipan, Commonwealth of the Northern Mariana Islands

    USGS Publications Warehouse

    Perreault, Jeff A.

    2007-01-01

    American Memorial Park, a unit of the National Park Service on the Island of Saipan, includes among its features a 27-acre estuarine system that has become a rarity within the Commonwealth of the Northern Mariana Islands. The estuarine system's mosaic of marshy areas interspersed with emergent wetlands and mixed wet forests provides critical habitat for various migratory and resident waterfowl, including two Federally listed endangered species: the Marianas gallinule (Gallinula chloropus guami) and the nightingale reed warbler (Acrocephalus luscinia). With sensitivity to the park's ecologic assets and the uncertainty associated with locally rapid urbanization, a need to better understand the hydrology of American Memorial Park was recognized. To address that need, a reconnaissance study of the park was undertaken during August and September 2005. The goals of the study were (1) to describe the occurrence and salinity of surface and ground water within the park; (2) to develop a hydrologic model of the park area of the island, with emphasis on the 27-acre estuarine system; and (3) to identify additional data needed to further develop this model. With regard to surface water, three freshwater inputs to the park's natural wetland are possible: direct rainfall, seaward-flowing ground water, and overland flow. Direct rainfall, which is an important source of freshwater to the wetland, commonly exceeds evapotranspiration both seasonally and per storm. The seaward flow of ground water is likely to be a source of freshwater to the wetland because ground water generally has an upward vertical component in the nearshore environment. Overland flow upgradient of the park could potentially contribute a significant input of freshwater during periods of intense rainfall, but roads that flank the park's perimeter act as a barrier to surficial inflows. During the reconnaissance, four discrete bodies, or zones, of surface water were observed within the park's natural wetland

  12. Nature and distribution of slab-derived fluids and mantle sources beneath the Southeast Mariana forearc rift

    NASA Astrophysics Data System (ADS)

    Ribeiro, Julia M.; Stern, Robert J.; Kelley, Katherine A.; Martinez, Fernando; Ishizuka, Osamu; Manton, William I.; Ohara, Yasuhiko

    2013-10-01

    Subduction zone magmas are produced by melting depleted mantle metasomatized by fluids released from the subducted slab. In most subduction zones, formation of backarc basin (BAB) and arc magmas depletes the mantle source toward the trench, resulting in more depleted mantle beneath the forearc. Slab-derived fluids are aqueous beneath the forearc where the slab dehydrates, and the deeper subduction component is increasingly dominated by sediment melt at ≥100 km depth. In this study, we present new data for the Southeast Mariana forearc rift (SEMFR), an unusual region of forearc igneous activity, where 2.7-3.7 Ma lavas were recovered by Shinkai 6500 diving and dredged during the TN273 Thomas Thompson cruise. SEMFR is divided into SE (near the trench) and NW (near the arc) sectors. NW SEMFR lavas and glassy rinds are more depleted in melt-mobile elements (e.g., Nb and Yb) and more enriched in fluid-mobile elements (e.g., Cs, Rb, and Ba). SEMFR lavas were produced by partial melting of a BAB-like mantle source, metasomatized by sediment melt and aqueous fluids released from dehydrating the subducted oceanic crust, and the forearc serpentinized peridotites. Evidence of sediment melt, even in SE SEMFR lavas, could be explained by inheritance of BAB-like Th/Nb in the SEMFR mantle source. Geochemical mapping demonstrates that the subduction components and mantle depletion increase towards the arc, suggesting (i) input of a less-depleted mantle beneath SE SEMFR that flowed toward the arc and (ii) aqueous slab-derived fluids become increasingly important at ˜50-100 km depth, reflecting that phengite and barite from the downgoing plate and forearc serpentinite broke down beneath the arc volcanoes.

  13. Appearance of Iron-based Microbial Ecosystems on and below the Seafloor: a Case Study of the Southern Mariana Trough

    NASA Astrophysics Data System (ADS)

    Kato, S.; Kentaro, K.; Toki, T.; Ishibashi, J.; Tsunogai, U.; Hirota, A.; Suzuki, K.; Moriya, O.; Yamagishi, A.

    2012-12-01

    Microbial community structures in deep-sea hydrothermal vents fields are constrained by available energy yields provided by inorganic redox reactions, which are in turn controlled by chemical composition of hydrothermal fluids. To date, geochemical and microbiological studies have been conducted in deep-sea hydrothermal vents at three geographically different areas (Snail, Archaean and Pika sites) of the Southern Mariana Trough (SMT). A variety of geochemical data of hydrothermal fluids and an unparalleled microbiological data of various samples (i.e., sulfide structures of active vents, iron-rich microbial mats, borehole fluids and surrounding bottom seawater) are available for comparative analyses. In the present study, we summarize these geochemical and microbiological characteristics and assess the relationship between the microbial community structures and the fluid geochemistry in the SMT by thermodynamic modeling. In the high-temperature vent fluids, aerobic sulfide-oxidation has the potential to yield large amounts of bioavailable energy in the vent fluids, which is consistent with the detection of species related to sulfide-oxidizing bacteria (such as Thiomicrospira in the Gammaproteobacteria and Sulfurimonas in the Epsilonproteobacteria). In contrast, the bioavailable energy yield from aerobic iron-oxidation reactions in the low-temperature fluids collected from man-made boreholes and several natural vents were comparable to or higher than those from sulfide-oxidation. This is consistent with the detection of species related to iron-oxidizing bacteria (Mariprofundus in the Zetaproteobacteria) in these low-temperature samples. These results provide novel insights into the presence and significance of iron-based microbial ecosystems in deep-sea hydrothermal fields.

  14. Long period seismicity and very long period infrasound driven by shallow magmatic degassing at Mount Pagan, Mariana Islands

    USGS Publications Warehouse

    Lyons, John; Haney, Matt; Werner, Cynthia A.; Kelly, Peter; Patrick, Matthew R.; Kern, Christoph; Trusdell, Frank A.

    2016-01-01

    Long period (LP) seismicity and very long period infrasound (iVLP) were recorded during continuous degassing from Mount Pagan, Mariana Islands, in July 2013 to January 2014. The frequency content of the LP and iVLP events and delay times between the two arrivals were remarkably stable and indicate nearly co-located sources. Using phase-weighted stacking over similar events to dampen noise, we find that the LP source centroid is located 60 m below and 180 m west of the summit vent. The moment tensor reveals a volumetric source modeled as resonance of a subhorizontal sill intersecting a dike. We model the seismoacoustic wavefields with a coupled earth-air 3-D finite difference code. The ratios of pressure to velocity measured at the infrasound arrays are an order of magnitude larger than the synthetic ratios, so the iVLP is not the result of LP energy transmitting into the atmosphere at its epicenter. Based on crater shape and dimensions determined by structure from motion, we model the iVLP as acoustic resonance of an exponential horn. The source of the continuous plume from gas analysis is shallow magmatic degassing, which repeatedly pressurized the dike-sill portion of the conduit over the 7 months of observation. Periodic gas release caused the geologically controlled sill to partially collapse and resonate, while venting of gas at the surface triggered resonance in the crater. LP degassing only accounts for ~12% of total degassing, indicating that most degassing is relatively aseismic and that multiple active pathways exist beneath the vent.

  15. Hydroacoustic records and a numerical model of the source mechanism from the first historical eruption of Anatahan Volcano, Mariana Islands

    NASA Astrophysics Data System (ADS)

    Dziak, R. P.; Park, M.; Matsumoto, H.; Byun, S.-K.

    2005-08-01

    Anatahan Volcano in the Commonwealth of the Northern Mariana Islands (CNMI) erupted for the first time in recorded history on 10 May 2003. The underwater acoustic records ( T-waves) of earthquakes, explosions, and tremor produced during the eruption were recorded on a sound channel hydrophone deployed in February 2003. Acoustic propagation models show that the seismic to acoustic conversion at Anatahan is particularly efficient, aided by the upward slope of the seamount toward the hydrophone. The hydrophone records confirm the onset of earthquake activity between 0100 and 0200Z on 10 May, with a substantial increase in seismicity beginning at ˜ 0620Z. In addition, the onset of continuous, low-frequency (3-40 Hz) acoustic energy that is likely volcanic tremor related to magma intrusion was also observed at 0620Z. The hydrophone recorded 1401 earthquakes during the first 3 days of the eruption. A histogram of seismicity indicates two main periods of explosion/eruption activity, the first beginning at ˜ 0620Z on 10 May and the second at ˜ 0000Z on 11 May. Relative earthquake depth estimates indicate that both eruption periods were accompanied by earthquake activity from deep within the Anatahan volcanic edifice. A numerical representation of the Anatahan volcano-seismic source was developed to examine the character of acoustic signals generated from the eruption governed by the geometry of the source and the physical properties of the magma. A magma pipe source mechanism is used to compute the seismo-acoustic wavefield on the flank of the Anatahan volcanic edifice (on the seafloor and in the water column) due to mode conversion by roughness scattering. A fluid-filled pipe model was chosen because it allows for a more straightforward relation between volcano geometry and spectral features of harmonic tremor as well as its morphologic similarity to a submerged volcanic edifice.

  16. A Comparison of MODIS and DOAS Sulfur Dioxide Measurements of the April 24, 2004 Eruption of Anatahan Volcano, Mariana Islands

    NASA Astrophysics Data System (ADS)

    Meier, V. L.; Scuderi, L.; Fischer, T.; Realmuto, V.; Hilton, D.

    2006-12-01

    Measurements of volcanic SO2 emissions provide insight into the processes working below a volcano, which can presage volcanic events. Being able to measure SO2 in near real-time is invaluable for the planning and response of hazard mitigation teams. Currently, there are several methods used to quantify the SO2 output of degassing volcanoes. Ground and aerial-based measurements using the differential optical absorption spectrometer (mini-DOAS) provide real-time estimates of SO2 output. Satellite-based measurements, which can provide similar estimates in near real-time, have increasingly been used as a tool for volcanic monitoring. Direct Broadcast (DB) real-time processing of remotely sensed data from NASA's Earth Observing System (EOS) satellites (MODIS Terra and Aqua) presents volcanologists with a range of spectral bands and processing options for the study of volcanic emissions. While the spatial resolution of MODIS is 1 km in the Very Near Infrared (VNIR) and Thermal Infrared (TIR), a high temporal resolution and a wide range of radiance measurements in 32 channels between VNIR and TIR combine to provide a versatile space borne platform to monitor SO2 emissions from volcanoes. An important question remaining to be answered is how well do MODIS SO2 estimates compare with DOAS estimates? In 2004 ground-based plume measurements were collected on April 24th and 25th at Anatahan volcano in the Mariana Islands using a mini-DOAS (Fischer and Hilton). SO2 measurements for these same dates have also been calculated using MODIS images and SO2 mapping software (Realmuto). A comparison of these different approaches to the measurement of SO2 for the same plume is presented. Differences in these observations are used to better quantify SO2 emissions, to assess the current mismatch between ground based and remotely sensed retrievals, and to develop an approach to continuously and accurately monitor volcanic activity from space in near real-time.

  17. Constraints on the diversity and distribution of coral-reef assemblages in the volcanic Northern Mariana Islands

    NASA Astrophysics Data System (ADS)

    Houk, P.; Starmer, J.

    2010-03-01

    A central problem for jurisdictional scientists and managers is to reconcile how multiple environmental regimes, encompassing continuous, intermittent and human disturbances, influence pertinent ecological management targets. The presence of heterogeneous environments throughout the volcanic Northern Mariana Islands (NMI), coupled with the availability of descriptive physical data, form the basis examining environmental-ecological relationships. Since 2003, coral abundances and macrobiota (all visibly recognizable taxa greater than 2 cm) occurrences have been estimated at 42 reef slopes along the volcanic archipelago. Analyses showed that reef types acted as surrogates of coral growth capacity and the modern assemblages residing upon them, being highest and most favorable, respectively, where relatively high salinity levels, low-to-moderate wave exposure, and an absence of volcanic activity for ~90 years existed. However, island size was the greatest constraint on species richness overall, but relations with corals were dampened by volcanic activity and increased for sponges and algae where greater connection with the island aquifer existed (i.e., relatively low salinity levels). The number of years since volcanic activity has occurred was positively related to the residuals of species-area relationships and coral cover, with a ~90-year time frame predicted for recovery. Notably, no relationships with watershed characteristics or distance from CNMI’s main fishing port and coral-reef assemblages or species richness were found. Further examination of specific management concerns, such as fisheries and feral animal populations, should be designed to account for the inherent differences in driving environmental regimes. Management strategies focused upon conserving biodiversity and ecosystem function should be centered at the island level, matching the operational scale of dominant environmental-ecological relationships. Marine reserves represent a strategy pertinent

  18. Source Evolution After Subduction Initiation as Recorded in the Izu-Bonin-Mariana Fore-arc Crust

    NASA Astrophysics Data System (ADS)

    Shervais, J. W.; Reagan, M. K.; Pearce, J. A.; Shimizu, K.

    2015-12-01

    Drilling in the Izu-Bonin-Mariana (IBM) fore-arc during IODP Expedition 352 and DSDP Leg 60 recovered consistent stratigraphic sequences of volcanic rocks reminiscent of those found in many ophiolites. The oldest lavas in these sections are "fore-arc basalts" (FAB) with ~51.5 Ma ages. Boninites began eruption approximately 2-3 m.y. later (Ishizuka et al., 2011, EPSL; Reagan et al., 2013, EPSL) and further from the trench. First results from IODP Expedition 352 and preliminary post-cruise data suggest that FAB at Sites U1440 and U1441 were generated by decompression melting during near-trench sea-floor spreading, and that fluids from the subducting slab were not involved in their genesis. Temperatures appear to have been unusually high and pressures of melting appear to have been unusually low compared to mid-ocean ridges. Spreading rates at this time appear to have been robust enough to maintain a stable melt lens. Incompatible trace element abundances are low in FAB compared to even depleted MORB. Nd and Hf Isotopic compositions published before the expedition suggest that FAB were derived from typical MORB source mantle. Thus, their extreme deletion resulted from unusually high degrees of melting immediately after subduction initiation. The oldest boninites from DSDP Site 458 and IODP Sites U1439 and U1442 have relatively high concentrations of fluid-soluble elements, low concentrations of REE, and light depleted REE patterns. Younger boninites, have even lower REE concentrations, but have U-shaped REE patterns. Our first major and trace element compositions for the FAB through boninite sequence suggests that melting pressures and temperatures decreased through time, mantle became more depleted though time, and spreading rates waned during boninite genesis. Subduction zone fluids involved in boninite genesis appear to have been derived from progressively higher temperatures and pressures over time as the subducting slab thermally matured.

  19. A Synthesis of Multibeam Bathymetry and Backscatter, and Sidescan Sonar of the Mariana Submarine Magmatic Arc, Western Pacific

    NASA Astrophysics Data System (ADS)

    Embley, R. W.; Chadwick, W. W.; Stern, R. J.; Merle, S. G.; Bloomer, S. H.; Nakamura, K.; Tamura, Y.

    2006-12-01

    A series of expeditions funded by the NSF MARGINS Program, the NOAA Ocean Exploration Program and JAMSTEC from 2001 to 2006 have collected a large data set that includes multibeam bathymetry and backscatter and surface-towed MR-1 sidescan sonar along more than 1200 km of the Mariana submarine magmatic arc between 12 and 23 degrees N. These data, along with more limited in situ observational data from remotely operated vehicle dives, are interpreted within an ESRI ArcGIS framework to produce a series of preliminary geologic maps showing a wide range of volcanic, tectonic and other morphologic features along the arc. The maps reveal a widespread channelized distribution of volcaniclastic material from volcanoes in the Southern Seamount Province (SSP) and Central Island Provinces (CIP). Giant sediment wave forms (up to 2 km in wavelength) occur on the flanks of many of the volcanoes and can be traced out to up to 60 km (Esmeralda). Relative ages of the activity of the volcanoes can be assigned in some areas where deposits from one edifice overlap others or where erosion has deeply dissected the flanks. Faulting is common on the frontal arc, especially in the SSP and the Northern Seamount Province (NSP). Many of the individual volcanoes have faults that are oriented at a range of azimuths but are commonly at either an arc-parallel or arc- orthogonal orientations. Several large extensional basins (>30 across in arc-parallel direction) occur in the NSP. One of these features exhibits a zone of headless channels on its western flank, implying that the feature may be a very large caldera.

  20. Two isotopically distinct fluid components involved in the Mariana arc: Evidence from Nb/B ratios and B, Sr, Nd, and Pb isotope systematics

    NASA Astrophysics Data System (ADS)

    Ishikawa, Tsuyoshi; Tera, Fouad

    1999-01-01

    Nb/B ratios and δ11B values of Mariana lavas reveal the involvement of two isotopically distinct slab-derived fluids in the magma genesis. The evidence of one type of fluid is observed in lavas of the islands of Anatahan, Alamagan, Agrigan, and Uracas, in which simple mixing between fluid with very homogeneous δ11B values (+5.40‰ ± 0.05‰) and the mantle wedge controls the magma compositions. In lavas of the islands of Guguan, Asuncion, and Pagan, however, the fluids that mixed with the mantle wedge are significantly heterogeneous in δ11B (+5.4‰ to +7.6‰) even within a single island. The compositional difference between these two types of fluid can result from different ratios of sediment to altered oceanic crust (AOC) in the slabs, and this hypothesis is consistent with the Sr, Nd, and Pb isotope data. We propose that the variable isotope compositions of the latter fluid may be derived from local changes in sediment/AOC ratios of the slab that are caused by subduction of the Magellan seamounts beneath the Mariana arc.

  1. Composition and spatial evolution of mantle and fluids released beneath the active Southeast Mariana Forearc Rift: do they have arc or backarc basin signatures?

    NASA Astrophysics Data System (ADS)

    Ribeiro, J. M.; Stern, R. J.; Kelley, K. A.; Ishizuka, O.; Anthony, E. Y.; Ren, M.; Manton, W. I.; Ohara, Y.; Reagan, M. K.; Bloomer, S. H.

    2010-12-01

    Fluids of progressively changing composition are released from the subducting slab. Whereas the composition and effects of deep fluids are understood from studying arcs and backarc basin (BAB) lavas, those released at shallower depths beneath forearcs are less well known. Forearc rifts give us a unique opportunity to study the composition of ultra-shallow subduction-related fluids. At the southern end of the Mariana arc, the S.E. Mariana Forearc Rift (SEMFR), was discovered by HMR-1 sonar swath mapping (Martinez et al. 2000, JGR), and investigated in July 2008 by the manned submersible Shinkai 6500. The rift extends from the trench to the BAB spreading axis, where a magma chamber was recently documented (Becker et al., 2010, G-cubed). SEMFR is opening due to continued widening of the Mariana Trough BAB. Two suites of tholeiitic pillow lavas were recovered from the N.E. flank of the rift (dive 1096; slab depth ~ 30 ± 5 km), indicating recent magmatic activity. Dive 1096 lavas consist of upper primitive basalts (Mg# ≥ 60) and lower fractionated, basaltic andesites (Mg# < 60), separated by a thin sediment layer. Geochemical and isotopic studies show that these lavas were produced by extensive hydrous melting (≥ 15%) of a common depleted MORB-like mantle (Nb/Yb ~ 1, ɛNd ~ 9.3), likely S. Mariana BAB mantle, that interacted with < 3% metasomatic fluids. Thermobarometry constraints (Lee et al., 2009, EPSL) suggest that the primary melts equilibrated with the mantle at ~ 28 km, just above the slab, with a mean temperature ~1230°C. The fluid was enriched in fluid-mobile elements (Rb, Ba, K, U, Sr, Pb, Cs), mobilized from the ultra-shallow slab at low temperature, as well as melt-mobile elements (e.g. Th, LREE), released deeper and hotter. These fluids contribute 100% Cs, 97% Rb, 99% Ba, 69% Th, 74% U, 80% K, 83% Pb, 71% Sr, 45% La, 33% Ce, 20% Nd and 11% Sm to the magma. SEMFR lavas acquired BAB-like deep subduction component as well as arc-like ultra

  2. Changes in the Metabolome of Picea balfouriana Embryogenic Tissues That Were Linked to Different Levels of 6-BAP by Gas Chromatography-Mass Spectrometry Approach

    PubMed Central

    Li, Q. F.; Wang, J. H.; Pulkkinen, P.; Kong, L. S.

    2015-01-01

    Embryogenic cultures of Picea balfouriana, which is an important commercial species for reforestation in Southern China, easily lose their embryogenic ability during long-term culture. Embryogenic tissue that proliferated at lower concentrations (3.6 μM and 2.5 μM) of 6-benzylaminopurine (6-BAP) were more productive, and generated 113 ± 6 and 89 ± 3 mature embryos per 100 mg embryogenic tissue, respectively. A metabolomic approach was used to study the changes in metabolites linked to embryogenic competence related to three different 6-BAP concentrations (2.5 μM, 3.6 μM, and 5 μM). A total of 309 compounds were obtained, among which 123 metabolites mapped to Kyoto Encyclopedia of Genes and genomes (KEGG) pathways. The levels of 35 metabolites were significantly differentially regulated among the three 6-BAP treatments, and 32 metabolites differed between the 2.5 μM and 5 μM treatments. A total of 17 metabolites appeared only once among the three comparisons. The combination of a score plot and a loading plot showed that in the samples with higher embryogenic ability (3.6 μM and 2.5 μM), up-regulated metabolites were mostly amino acids and down-regulated metabolites were mostly primary carbohydrates (especially sugars). These results suggested that 6-BAP may influence embryogenic competence by nitrogen metabolism, which could cause an increase in amino acid levels and higher amounts of aspartate, isoleucine, and leucine in tissues with higher embryogenic ability. Furthermore, we speculated that 6-BAP may affect the amount of tryptophan in tissues, which would change the indole-3-acetic acid levels and influence the embryogenic ability. PMID:26517840

  3. Morphological plasticity of ectomycorrhizal short roots in Betula sp and Picea abies forests across climate and forest succession gradients: its role in changing environments.

    PubMed

    Ostonen, Ivika; Rosenvald, Katrin; Helmisaari, Heljä-Sisko; Godbold, Douglas; Parts, Kaarin; Uri, Veiko; Lõhmus, Krista

    2013-01-01

    Morphological plasticity of ectomycorrhizal (EcM) short roots (known also as first and second order roots with primary development) allows trees to adjust their water and nutrient uptake to local environmental conditions. The morphological traits (MTs) of short-living EcM roots, such as specific root length (SRL) and area, root tip frequency per mass unit (RTF), root tissue density, as well as mean diameter, length, and mass of the root tips, are good indicators of acclimation. We investigated the role of EcM root morphological plasticity across the climate gradient (48-68°N) in Norway spruce (Picea abies (L.) Karst) and (53-66°N) birch (Betula pendula Roth., B. pubescens Ehrh.) forests, as well as in primary and secondary successional birch forests assuming higher plasticity of a respective root trait to reflect higher relevance of that characteristic in acclimation process. We hypothesized that although the morphological plasticity of EcM roots is subject to the abiotic and biotic environmental conditions in the changing climate; the tools to achieve the appropriate morphological acclimation are tree species-specific. Long-term (1994-2010) measurements of EcM roots morphology strongly imply that tree species have different acclimation-indicative root traits in response to changing environments. Birch EcM roots acclimated along latitude by changing mostly SRL [plasticity index (PI) = 0.60], while spruce EcM roots became adjusted by modifying RTF (PI = 0.68). Silver birch as a pioneer species must have a broader tolerance to environmental conditions across various environments; however, the mean PI of all MTs did not differ between early-successional birch and late-successional spruce. The differences between species in SRL, and RTF, diameter, and length decreased southward, toward temperate forests with more favorable growth conditions. EcM root traits reflected root-rhizosphere succession across forest succession stages.

  4. Throughfall deposition and canopy exchange processes along a vertical gradient within the canopy of beech (Fagus sylvatica L.) and Norway spruce (Picea abies (L.) Karst).

    PubMed

    Adriaenssens, Sandy; Hansen, Karin; Staelens, Jeroen; Wuyts, Karen; De Schrijver, An; Baeten, Lander; Boeckx, Pascal; Samson, Roeland; Verheyen, Kris

    2012-03-15

    To assess the impact of air pollution on forest ecosystems, the canopy is usually considered as a constant single layer in interaction with the atmosphere and incident rain, which could influence the measurement accuracy. In this study the variation of througfall deposition and derived dry deposition and canopy exchange were studied along a vertical gradient in the canopy of one European beech (Fagus sylvatica L.) tree and two Norway spruce (Picea abies (L.) Karst) trees. Throughfall and net throughfall deposition of all ions other than H(+) increased significantly with canopy depth in the middle and lower canopy of the beech tree and in the whole canopy of the spruce trees. Moreover, throughfall and net throughfall of all ions in the spruce canopy decreased with increasing distance to the trunk. Dry deposition occurred mainly in the upper canopy and was highest during the growing season for H(+), NH(4)(+), NO(3)(-) and highest during the dormant season for Na(+), Cl(-), SO(4)(2-) (beech and spruce) and K(+), Ca(2+) and Mg(2+) (spruce only). Canopy leaching of K(+), Ca(2+) and Mg(2+) was observed at all canopy levels and was higher for the beech tree compared to the spruce trees. Canopy uptake of inorganic nitrogen and H(+) occurred mainly in the upper canopy, although significant canopy uptake was found in the middle canopy as well. Canopy exchange was always higher during the growing season compared to the dormant season. This spatial and temporal variation indicates that biogeochemical deposition models would benefit from a multilayer approach for shade-tolerant tree species such as beech and spruce.

  5. Assessing the impacts of climate change and nitrogen deposition on Norway spruce (Picea abies L. Karst) growth in Austria with BIOME-BGC.

    PubMed

    Eastaugh, Chris S; Pötzelsberger, Elisabeth; Hasenauer, Hubert

    2011-03-01

    The aim of this paper is to determine whether a detectable impact of climate change is apparent in Austrian forests. In regions of complex terrain such as most of Austria, climatic trends over the past 50 years show marked geographic variability. As climate is one of the key drivers of forest growth, a comparison of growth characteristics between regions with different trends in temperature and precipitation can give insights into the impact of climatic change on forests. This study uses data from several hundred climate recording stations, interpolated to measurement sites of the Austrian National Forest Inventory (NFI). Austria as a whole shows a warming trend over the past 50 years and little overall change in precipitation. The warming trends, however, vary considerably across certain regions and regional precipitation trends vary widely in both directions, which cancel out on the national scale These differences allow the delineation of 'climatic change zones' with internally consistent climatic trends that differ from other zones. This study applies the species-specific adaptation of the biogeochemical model BIOME-BGC to Norway spruce (Picea abies (L.) Karst) across a range of Austrian climatic change zones, using input data from a number of national databases. The relative influence of extant climate change on forest growth is quantified, and compared with the far greater impact of non-climatic factors. At the national scale, climate change is found to have negligible effect on Norway spruce productivity, due in part to opposing effects at the regional level. The magnitudes of the modeled non-climatic influences on aboveground woody biomass increment increases are consistent with previously reported values of 20-40 kg of added stem carbon sequestration per kilogram of additional nitrogen deposition, while climate responses are of a magnitude difficult to detect in NFI data.

  6. Pliocene intraspecific divergence and Plio-Pleistocene range expansions within Picea likiangensis (Lijiang spruce), a dominant forest tree of the Qinghai-Tibet Plateau.

    PubMed

    Li, Long; Abbott, Richard J; Liu, Bingbing; Sun, Yongshuai; Li, Lili; Zou, Jiabin; Wang, Xi; Miehe, Georg; Liu, Jianquan

    2013-10-01

    A knowledge of intraspecific divergence and range dynamics of dominant forest trees in response to past geological and climate change is of major importance to an understanding of their recent evolution and demography. Such knowledge is informative of how forests were affected by environmental factors in the past and may provide pointers to their response to future environmental change. However, genetic signatures of such historical events are often weak at individual loci due to large effective population sizes and long generation times of forest trees. This problem can be overcome by analysing genetic variation across multiple loci. We used this approach to examine intraspecific divergence and past range dynamics in the conifer Picea likiangensis, a dominant tree of forests occurring in eastern and southern areas of the Qinghai-Tibet Plateau (QTP). We sequenced 13 nuclear loci, two mitochondrial DNA regions and three plastid (chloroplast) DNA regions in 177 individuals sampled from 22 natural populations of this species, and tested the hypothesis that its evolutionary history was markedly affected by Pliocene QTP uplifts and Quaternary climatic oscillations. Consistent with the taxonomic delimitation of the three morphologically divergent varieties examined, all individuals clustered into three genetic groups with intervariety admixture detected in regions of geographical overlap. Divergence between varieties was estimated to have occurred within the Pliocene and ecological niche modelling based on 20 ecological variables suggested that niche differentiation was high. Furthermore, modelling of population-genetic data indicated that two of the varieties (var. rubescens and var. linzhiensis) expanded their population sizes after the largest Quaternary glaciation in the QTP, while expansion of the third variety (var. likiangensis) began prior to this, probably following the Pliocene QTP uplift. These findings point to the importance of geological and climatic

  7. Disentangling the roles of history and local selection in shaping clinal variation of allele frequencies and gene expression in Norway spruce (Picea abies).

    PubMed

    Chen, Jun; Källman, Thomas; Ma, Xiaofei; Gyllenstrand, Niclas; Zaina, Giusi; Morgante, Michele; Bousquet, Jean; Eckert, Andrew; Wegrzyn, Jill; Neale, David; Lagercrantz, Ulf; Lascoux, Martin

    2012-07-01

    Understanding the genetic basis of local adaptation is challenging due to the subtle balance among conflicting evolutionary forces that are involved in its establishment and maintenance. One system with which to tease apart these difficulties is clines in adaptive characters. Here we analyzed genetic and phenotypic variation in bud set, a highly heritable and adaptive trait, among 18 populations of Norway spruce (Picea abies), arrayed along a latitudinal gradient ranging from 47°N to 68°N. We confirmed that variation in bud set is strongly clinal, using a subset of five populations. Genotypes for 137 single-nucleotide polymorphisms (SNPs) chosen from 18 candidate genes putatively affecting bud set and 308 control SNPs chosen from 264 random genes were analyzed for patterns of genetic structure and correlation to environment. Population genetic structure was low (F(ST) = 0.05), but latitudinal patterns were apparent among Scandinavian populations. Hence, part of the observed clinal variation should be attributable to population demography. Conditional on patterns of genetic structure, there was enrichment of SNPs within candidate genes for correlations with latitude. Twenty-nine SNPs were also outliers with respect to F(ST). The enrichment for clinal variation at SNPs within candidate genes (i.e., SNPs in PaGI, PaPhyP, PaPhyN, PaPRR7, and PaFTL2) indicated that local selection in the 18 populations, and/or selection in the ancestral populations from which they were recently derived, shaped the observed cline. Validation of these genes using expression studies also revealed that PaFTL2 expression is significantly associated with latitude, thereby confirming the central role played by this gene in the control of phenology in plants.

  8. Structural studies of TiO2/wood coatings prepared by hydrothermal deposition of rutile particles from TiCl4 aqueous solutions on spruce (Picea Abies) wood

    NASA Astrophysics Data System (ADS)

    Pori, Pavel; Vilčnik, Aljaž; Petrič, Marko; Sever Škapin, Andrijana; Mihelčič, Mohor; Šurca Vuk, Angela; Novak, Urban; Orel, Boris

    2016-05-01

    A low temperature approach was developed for the deposition of rutile TiO2 particles on a wood surface by hydrolysis of TiCl4 in aqueous solutions acidified with HCl, and crystallization at 75 and 90 °C (1 h). Prior to hydrothermal treatment, Picea Abies wood was first soaked in a 0.5 mmol/l aqueous solution containing anionic surfactant sodium dodecyl sulphate (SDS, Sigma Aldrich) for 2 h at 80 °C. The crystal structure of the hydrothermally made rutile particles was determined with XRD, while the morphology of the deposited TiO2 particles and their distribution in the wood were examined with SEM and EDX measurements. The penetration and amount of deposited rutile particles could be modified by changing the deposition conditions. Thicker layers were obtained from more concentrated aqueous TiCl4 solutions with and without added HCl, and with longer deposition times and higher temperatures of the hydrothermal treatment. The interaction of TiO2 particles with hemicellulose and lignin in wood was established from infrared attenuated total reflection (FT-IR ATR) and Raman spectra measurements, from which the spectra of wood were subtracted. Analysis of the subtraction spectra showed the presence of titania particles on the wood surface, revealing also the establishment of TiO2-wood coordinative bonds of titanium ions with hemicellulose and lignin. The red frequency shift of the OH stretching modes suggested interaction of the TiO2 particles with water molecules of wood. TiO2 deposited on wood treated with SDS became hydrophobic (water contact angles (WCA) of 150°), contrasting the properties of untreated wood with a deposited TiO2 particle coating, which remained hydrophilic.

  9. Mutational analysis of white spruce (Picea glauca) ent-kaurene synthase (PgKS) reveals common and distinct mechanisms of conifer diterpene synthases of general and specialized metabolism.

    PubMed

    Zerbe, Philipp; Chiang, Angela; Bohlmann, Jörg

    2012-02-01

    Conifer diterpene synthases (diTPSs) catalyze the multi-step cycloisomerization of geranylgeranyl diphosphate, or copalyl diphosphate, to a variety of diterpenes in general (i.e., primary) and specialized (i.e., secondary) metabolism. Despite their functional diversity, the known conifer diTPSs are structurally closely related, with variations in three conserved domains, α, β and γ. The catalytic specificity of conifer class I and class I/II diTPSs is predominantly determined by the protein environment of the C-terminal class I active site through stabilization of common and unique carbocation intermediates. Using the crystal structure of Taxus brevifolia taxadiene synthase as template, comparative modeling and mutagenesis of the class I diTPS ent-kaurene synthase from Picea glauca (PgKS) was performed to elucidate the catalytic specificity of PgKS relative to spruce diTPSs of specialized metabolism. N-terminal truncations demonstrated a role for the βγ domain in class I enzyme activity for PgKS, facilitating the closure of the class I active site upon substrate binding. Based on position, Arg476 and Asp736 in the C-terminal α domain of PgKS may contribute to this conformational transition and appear critical for catalysis. Consistent with the mechanism of other diTPSs, the subsequent ionization of a copalyl diphosphate substrate and coordination of the diphosphate group is controlled by strictly conserved residues in the DDxxD and NDIQGCKRE motif of PgKS, such as Asn656 and Arg653. Furthermore, Lys478, Trp502, Met588, Ala615 and Ile619 control the enzymatic activity and specificity of PgKS via carbocation stabilization en route to ent-kaurene. These positions show a high level of amino acid variation, consistent with functional plasticity among conifer diTPSs of different functions in general or specialized metabolism.

  10. An integrated genomic, proteomic and biochemical analysis of (+)-3-carene biosynthesis in Sitka spruce (Picea sitchensis) genotypes that are resistant or susceptible to white pine weevil.

    PubMed

    Hall, Dawn E; Robert, Jeanne A; Keeling, Christopher I; Domanski, Dominik; Quesada, Alfonso Lara; Jancsik, Sharon; Kuzyk, Michael A; Hamberger, Britta; Borchers, Christoph H; Bohlmann, Jörg

    2011-03-01

    Conifers are extremely long-lived plants that have evolved complex chemical defenses in the form of oleoresin terpenoids to resist attack from pathogens and herbivores. In these species, terpenoid diversity is determined by the size and composition of the terpene synthase (TPS) gene family and the single- and multi-product profiles of these enzymes. The monoterpene (+)-3-carene is associated with resistance of Sitka spruce (Picea sitchensis) to white pine weevil (Pissodes strobi). We used a combined genomic, proteomic and biochemical approach to analyze the (+)-3-carene phenotype in two contrasting Sitka spruce genotypes. Resistant trees produced significantly higher levels of (+)-3-carene than susceptible trees, in which only trace amounts were detected. Biosynthesis of (+)-3-carene is controlled, at the genome level, by a small family of closely related (+)-3-carene synthase (PsTPS-3car) genes (82-95% amino acid sequence identity). Transcript profiling identified one PsTPS-3car gene (PsTPS-3car1) that is expressed in both genotypes, one gene (PsTPS-3car2) that is expressed only in resistant trees, and one gene (PsTPS-3car3) that is expressed only in susceptible trees. The PsTPS-3car2 gene was not detected in genomic DNA of susceptible trees. Target-specific selected reaction monitoring confirmed this pattern of differential expression of members of the PsTPS-3car family at the proteome level. Kinetic characterization of the recombinant PsTPS-3car enzymes identified differences in the activities of PsTPS-3car2 and PsTPS-3car3 as a factor contributing to the different (+)-3-carene profiles of resistant and susceptible trees. In conclusion, variation of the (+)-3-carene phenotype is controlled by copy number variation of PsTPS-3car genes, variation of gene and protein expression, and variation in catalytic efficiencies.

  11. Photosynthesis and growth response of red spruce and loblolly pine to soil-applied lead and simulated acid rain. [Picea rubens; Pinus taeda

    SciTech Connect

    Seiler, J.R.; Paganelli, D.J.

    1987-09-01

    Soils from red spruce (Picea rubens Sarg.) and loblolly pine (Pinus taeda L.) stands were amended with either 0, 150, 300, 600 or 1200 mg/kg Pb as PbCl/sub 2/. Six-month-old spruce and six-week-old pine seedlings were planted in their respective native soils and treated for 19 weeks with simulated rain of either pH 4.5 or 3.0. Rain was applied directly to the soil at a rate of 1.5 cm per week. Net photosynthesis, height, and needle, shoot, and root dry weights were measured at the completion of the experiment. In both soils, pH decreased and nitrate concentration increased with the application of a simulated rain solution of pH 3.0. Despite these changes in soil chemistry, simulated rain pH had no significant effect on the growth of either species. Red spruce photosynthesis was 35% higher; however, at a pH of 3.0. Loblolly pine photosynthesis was not affected by solution pH. Growth and photosynthesis of red spruce were inhibited even at the 150 mg/kg Pb level, with additional Pb resulting in increasing inhibition. Growth of loblolly pine seedlings was less sensitive to Pb, and decreased only at the higher concentrations. Loblolly pine photosynthesis exhibited no decline even at the highest Pb level. These results suggest that both red spruce and loblolly pine are more sensitive to soil Pb than to acid precipitation. In addition, loblolly pine appears to be more tolerant of Pb than red spruce, when both species are grown in their respective native soils.

  12. Variation in light-intercepting area and photosynthetic rate of sun and shade shoots of two Picea species in relation to the angle of incoming light.

    PubMed

    Ishii, Hiroaki; Hamada, Yoko; Utsugi, Hajime

    2012-10-01

    We investigated the effects of sun- and shade-shoot architecture on the photosynthetic rates of two Picea species by applying light from various angles in the laboratory. Compared with sun shoots, shade shoots were characterized by lower mass allocation per light-intercepting area, less leaf mass per shoot mass, less mutual shading among leaves and more efficient allocation of chlorophyll to photosynthesis. The shoot silhouette to total leaf area ratio (STAR(ϕ)) decreased with increasing shoot inclination angle (ϕ, the shoot axis angle relative to the projection plane) and was consistently higher for the shade shoots. Morphological and physiological characteristics of the shade shoots resulted in maximum rates of net photosynthesis at ϕ = 0° (P(max,0)) similar to that of the sun shoots when expressed on a leaf mass, total leaf area and chlorophyll basis. When the angle of incoming light was varied, P(max,ϕ) per total leaf area (P(max,ϕ )/A(T)) of the shade shoots increased linearly with increasing STAR(ϕ), while P(max,ϕ) per shoot silhouette area did not change. In contrast, the response of the sun shoots was non-linear, and an optimum angle of incoming light was determined. Our results suggest that shade-shoot morphology is adaptive for utilizing diffuse light incoming from various angles, while sun-shoot morphology is adaptive for avoiding the negative effects of strong direct radiation and for enhancing light diffusion into the canopy. We propose that the angle of incoming light should be taken into account when estimating photosynthetic rates of sun shoots of conifer trees in the field.

  13. Online investigation of respiratory quotients in Pinus sylvestris and Picea abies during drought and shading by means of cavity-enhanced Raman multi-gas spectrometry.

    PubMed

    Hanf, Stefan; Fischer, Sarah; Hartmann, Henrik; Keiner, Robert; Trumbore, Susan; Popp, Jürgen; Frosch, Torsten

    2015-07-07

    Photosynthesis and respiration are major components of the plant carbon balance. During stress, like drought, carbohydrate supply from photosynthesis is reduced and the Krebs cycle respiration must be fueled with other stored carbon compounds. However, the dynamics of storage use are still unknown. The respiratory quotient (RQ, CO2 released per O2 consumed during respiration) is an excellent indicator of the nature of the respiration substrate. In plant science, however, online RQ measurements have been challenging or even impossible so far due to very small gas exchange fluxes during respiration. Here we apply cavity-enhanced multi-gas Raman spectrometry (CERS) for online in situ RQ measurements in drought-tolerant pine (Pinus sylvestris [L.]) and drought-intolerant spruce (Picea abies [L. H. Karst]). Two different treatments, drought and shading, were applied to reduce photosynthesis and force dependency on stored substrates. Changes in respiration rates and RQ values were continuously monitored over periods of several days with low levels of variance. The results show that both species switched from COH-dominated respiration (RQ = 1.0) to a mixture of substrates during shading (RQ = 0.77-0.81), while during drought only pine did so (RQ = 0.75). The gas phase measurements were complemented by concentration measurements of non-structural carbohydrates and lipids. These first results suggest a physiological explanation for greater drought tolerance in pine. CERS was proven as powerful technique for non-consumptive and precise real-time monitoring of respiration rates and respirational quotients for the investigation of plant metabolism under drought stress conditions that are predicted to increase with future climate change.

  14. Altitudinal vs Latitudinal Climactic Drivers: A Comparison of a Relict Picea and Abies Forest in the Southern Appalachians versus the Hemi-Boreal Transition Zone off Southern Canada

    NASA Astrophysics Data System (ADS)

    Evans, A.; Lafon, C. W.

    2015-12-01

    Identification of biotic and abiotic determinants of tree species range limits is critical for understanding the effects of climate change on species distributions. Upward shifts of species distributions in montane areas have been widely reported but there have been few reports of latitudinal range retractions. Previous studies have indicated that southern latitudinal limits of a species range are dictated by biotic factors such as competition while others have suggested that abiotic factors, such as temperature, dictate these limits. We investigated the potential climatic gradients at the southern latitudinal limit of the Spruce (Picea) and Fir (Abies) species that dominate the Canadian boreal forest community as well as relict boreal forests containing similar species found in the high elevation areas of the Southern Appalachians. Existing research has suggested that relict ecosystems are more sensitive to climate change and can be indicative of future changes at latitudinal range limits. Expanding on this literature, we hypothesized that we would see similar gradients in climatic variables at the southern latitudinal limit of the Canadian boreal forest and those in the relict boreal forests southern Appalachians acting as controlling factors of these species distributions. We used forty years of climate data from weather stations along the southern edge of the boreal forest in the Canadian Shield provinces, species distribution data from the Canadian National Forest Inventory, (CNFI) geospatial data from the National Park Service (NPS), and historical weather data from the National Oceanic and Atmospheric Administration (NOAA) to perform our analysis. Our results indicate different climate variables act as controls of warm edge range limits of the Canadian boreal forest than those of the relict boreal forest of the southern Appalachians. However, we believe range retractions of the relict forest may be indicative of a more gradual response of similar species

  15. Different Alleles of a Gene Encoding Leucoanthocyanidin Reductase (PaLAR3) Influence Resistance against the Fungus Heterobasidion parviporum in Picea abies.