Study Sheds New Light on Postmenopausal Breast Cancer Risk | Poster

Cancer.gov

A collaborative study conducted at the Frederick National Lab and published in Cancer Research has provided new insight as to why elevated estrogen levels are associated with an increased risk of breast cancer.

CFTR Modulators: Shedding Light on Precision Medicine for Cystic Fibrosis

PubMed Central

Lopes-Pacheco, Miquéias

2016-01-01

Cystic fibrosis (CF) is the most common life-threatening monogenic disease afflicting Caucasian people. It affects the respiratory, gastrointestinal, glandular and reproductive systems. The major cause of morbidity and mortality in CF is the respiratory disorder caused by a vicious cycle of obstruction of the airways, inflammation and infection that leads to epithelial damage, tissue remodeling and end-stage lung disease. Over the past decades, life expectancy of CF patients has increased due to early diagnosis and improved treatments; however, these patients still present limited quality of life. Many attempts have been made to rescue CF transmembrane conductance regulator (CFTR) expression, function and stability, thereby overcoming the molecular basis of CF. Gene and protein variances caused by CFTR mutants lead to different CF phenotypes, which then require different treatments to quell the patients’ debilitating symptoms. In order to seek better approaches to treat CF patients and maximize therapeutic effects, CFTR mutants have been stratified into six groups (although several of these mutations present pleiotropic defects). The research with CFTR modulators (read-through agents, correctors, potentiators, stabilizers and amplifiers) has achieved remarkable progress, and these drugs are translating into pharmaceuticals and personalized treatments for CF patients. This review summarizes the main molecular and clinical features of CF, emphasizes the latest clinical trials using CFTR modulators, sheds light on the molecular mechanisms underlying these new and emerging treatments, and discusses the major breakthroughs and challenges to treating all CF patients. PMID:27656143

Seeing an Old Lab in a New Light: Transforming a Traditional Optics Lab into Full Guided Inquiry

ERIC Educational Resources Information Center

Maley, Tim; Stoll, Will; Demir, Kadir

2013-01-01

This paper describes the authors' experiences transforming a "cookbook" lab into an inquiry-based investigation and the powerful effect the inquiry-oriented lab had on our students' understanding of lenses. We found the inquiry-oriented approach led to richer interactions between students as well as a deeper conceptual…

Photoreceptor disc shedding in the living human eye

PubMed Central

Kocaoglu, Omer P.; Liu, Zhuolin; Zhang, Furu; Kurokawa, Kazuhiro; Jonnal, Ravi S.; Miller, Donald T.

2016-01-01

Cone photoreceptors undergo a daily cycle of renewal and shedding of membranous discs in their outer segments (OS), the portion responsible for light capture. These physiological processes are fundamental to maintaining photoreceptor health, and their dysfunction is associated with numerous retinal diseases. While both processes have been extensively studied in animal models and postmortem eyes, little is known about them in the living eye, in particular human. In this study, we report discovery of the optical signature associated with disc shedding using a method based on adaptive optics optical coherence tomography (AO-OCT) in conjunction with post-processing methods to track and monitor individual cone cells in 4D. The optical signature of disc shedding is characterized by an abrupt transient loss in the cone outer segment tip (COST) reflection followed by its return that is axially displaced anteriorly. Using this signature, we measured the temporal and spatial properties of shedding events in three normal subjects. Average duration of the shedding event was 8.8 ± 13.4 minutes, and average length loss of the OS was 2.1 μm (7.0% of OS length). Prevalence of cone shedding was highest in the morning (14.3%) followed by the afternoon (5.7%) and evening (4.0%), with load distributed across the imaged patch. To the best of our knowledge these are the first images of photoreceptor disc shedding in the living retina. PMID:27895995

Shedding Light on Engineering Design

ERIC Educational Resources Information Center

Capobianco, Brenda M.; Nyquist, Chell; Tyrie, Nancy

2013-01-01

This article describes the steps incorporated to teach an engineering design process in a fifth-grade science classroom. The engineering design-based activity was an existing scientific inquiry activity using UV light--detecting beads and purposefully creating a series of engineering design-based challenges around the investigation. The…

Shedding Light on Fiber Optics.

ERIC Educational Resources Information Center

Bunch, Robert M.

1994-01-01

Explains the principles of fiber optics as a medium for light-wave communication. Current uses of fiber systems on college campuses include voice, video, and local area network applications. A group of seven school districts in Minnesota are linked via fiber-optic cables. Other uses are discussed. (MLF)

Rapid prototyping of an adaptive light-source for mobile manipulators with EasyKit and EasyLab

NASA Astrophysics Data System (ADS)

Wojtczyk, Martin; Barner, Simon; Geisinger, Michael; Knoll, Alois

2008-08-01

While still not common in day-to-day business, mobile robot platforms form a growing market in robotics. Mobile platforms equipped with a manipulator for increased flexibility have been used successfully in biotech laboratories for sample management as shown on the well-known ESACT meetings. Navigation and object recognition is carried out by the utilization of a mounted machine vision camera. To cope with the different illumination conditions in a large laboratory, development of an adaptive light source was indispensable. We present our approach of rapid developing a computer controlled, adaptive LED light within one single business day, by utilizing the hardware toolbox EasyKit and our appropriate software counterpart EasyLab.

Supercharging Lessons with a Virtual Lab

ERIC Educational Resources Information Center

Stewart, Jefferson; Vincent, Daniel

2013-01-01

The authors describes their experiences incorporating the virtual lab into a simple circuit lesson during an energy unit in a sixth-grade class. The lesson included a hands-on group experiment using wire, batteries, and light bulbs to make a circuit and an online simulation, using a virtual lab. Class discussions, student inquiries, and the study…

Shedding light on some possible remedies against watermark desynchronization: a case study

NASA Astrophysics Data System (ADS)

Barni, Mauro

2005-03-01

Watermark de-synchronization is perhaps the most dangerous attack against the great majority of watermarking systems proposed so far. Exhaustive search and template matching are two of the most popular solution against it, however several doubts exist about their effectiveness. As a matter of fact, a controversial point in digital watermarking is whether these techniques are of any help to cope with watermark de-synchronization introduced by geometric attacks. On one side, watermark synchronization through exhaustive search dramatically increases the false detection probability. On the other side, for the template matching approach the probability of a synchronization error must be taken into account, thus deteriorating significantly the performance of the system. It is the scope of this paper to shed some light on the above points. To do so we focus on a very simple case study, whereby we show that as long as the size of the search space (the cardinality of the geometric attack) increases polynomially with the length of the to-be-marked host feature sequence, both methods provide an effective solution to the de-synchronization problem. Interestingly, and rather surprisingly, we also show that Exhaustive Search Detection (ESD) always outperforms Template Matching Detection (TMD), though the general behavior of the two schemes is rather similar.

Genetic and Epigenetic Diversities Shed Light on Domestication of Cultivated Ginseng (Panax ginseng).

PubMed

Li, Ming-Rui; Shi, Feng-Xue; Zhou, Yu-Xin; Li, Ya-Ling; Wang, Xin-Feng; Zhang, Cui; Wang, Xu-Tong; Liu, Bao; Xiao, Hong-Xing; Li, Lin-Feng

2015-11-02

Chinese ginseng (Panax ginseng) is a medically important herb within Panax and has crucial cultural values in East Asia. As the symbol of traditional Chinese medicine, Chinese ginseng has been used as a herbal remedy to restore stamina and capacity in East Asia for thousands of years. To address the evolutionary origin and domestication history of cultivated ginseng, we employed multiple molecular approaches to investigate the genetic structures of cultivated and wild ginseng across their distribution ranges in northeastern Asia. Phylogenetic and population genetic analyses revealed that the four cultivated ginseng landraces, COMMON, BIANTIAO, SHIZHU, and GAOLI (also known as Korean ginseng), were not domesticated independently and Fusong Town is likely one of the primary domestication centers. In addition, our results from population genetic and epigenetic analyses demonstrated that cultivated ginseng maintained high levels of genetic and epigenetic diversity, but showed distinct cytosine methylation patterns compared with wild ginseng. The patterns of genetic and epigenetic variation revealed by this study have shed light on the domestication history of cultivated ginseng, which may serve as a framework for future genetic improvements. Copyright © 2015 The Author. Published by Elsevier Inc. All rights reserved.

7. Outhouse and shed, view west northwest, south and east ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

7. Outhouse and shed, view west northwest, south and east sides - Ram Island Light Station, Ram Island, south of Ocean Point & just north of Fisherman Island, marking south side of Fisherman Island Passage, Ocean Point, Lincoln County, ME

Shedding Some Light on Fluorescent Bulbs.

ERIC Educational Resources Information Center

Guilbert, Nicholas R.

1996-01-01

Explores some of the principles behind the working of fluorescent bulbs using a specially prepared fluorescent bulb with the white inner fluorescent coating applied along only half its length. Discusses the spectrum, the bulb plasma, and light production. (JRH)

7. Shed and keeper' house with helicopter pad in foreground, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

7. Shed and keeper' house with helicopter pad in foreground, view east, southwest and northwest sides - Goat Island Light Station, Goat Island, next to entrance to Cape Porpoise Harbor, just south of Trott Island, Cape Porpoise, York County, ME

Seventy Five Years of Particle Accelerators (LBNL Summer Lecture Series)

ScienceCinema

Sessler, Andy [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

2017-12-09

Summer Lecture Series 2006: Andy Sessler, Berkeley Lab director from 1973 to 1980, sheds light on the Lab's nearly eight-decade history of inventing and refining particle accelerators, which continue to illuminate the nature of the universe.

Advanced LabVIEW Labs

DOE Office of Scientific and Technical Information (OSTI.GOV)

Jones, Eric D.

1999-06-17

In the world of computer-based data acquisition and control, the graphical interface program LabVIEW from National Instruments is so ubiquitous that in many ways it has almost become the laboratory standard. To date, there have been approximately fifteen books concerning LabVIEW, but Professor Essick's treatise takes on a completely different tack than all of the previous discussions. In the more standard treatments of the ways and wherefores of LabVIEW such as LabVIEW Graphical Programming: Practical Applications in Instrumentation and Control by Gary W. Johnson (McGraw Hill, NY 1997), the emphasis has been instructing the reader how to program LabVIEW tomore » create a Virtual Instrument (VI) on the computer for interfacing to a particular instruments. LabVIEW is written in G a graphical programming language developed by National Instruments. In the past the emphasis has been on training the experimenter to learn G . Without going into details here, G incorporates the usual loops, arithmetic expressions, etc., found in many programming languages, but in an icon (graphical) environment. The net result being that LabVIEW contains all of the standard methods needed for interfacing to instruments, data acquisition, data analysis, graphics, and also methodology to incorporate programs written in other languages into LabVIEW. Historically, according to Professor Essick, he developed a series of experiments for an upper division laboratory course for computer-based instrumentation. His observation was that while many students had the necessary background in computer programming languages, there were students who had virtually no concept about writing a computer program let alone a computer- based interfacing program. Thus the beginnings of a concept for not only teaching computer- based instrumentation techniques, but aiso a method for the beginner to experience writing a com- puter program. Professor Essick saw LabVIEW as the perfect environment in which to teach

Advanced LabVIEW Labs

DOE Office of Scientific and Technical Information (OSTI.GOV)

Jones, Eric D.

1999-06-17

In the world of computer-based data acquisition and control, the graphical interface program LabVIEW from National Instruments is so ubiquitous that in many ways it has almost become the laboratory standard. To date, there have been approximately fifteen books concerning LabVIEW, but Professor Essick's treatise takes on a completely different tack than all of the previous discussions. In the more standard treatments of the ways and wherefores of LabVIEW such as LabVIEW Graphical Programming: Practical Applications in Instrumentation and Control by Gary W. Johnson (McGraw Hill, NY 1997), the emphasis has been instructing the reader how to program LabVIEW tomore » create a Virtual Instrument (VI) on the computer for interfacing to a particular instruments. LabVIEW is written in "G" a graphical programming language developed by National Instruments. In the past the emphasis has been on training the experimenter to learn "G". Without going into details here, "G" incorporates the usual loops, arithmetic expressions, etc., found in many programming languages, but in an icon (graphical) environment. The net result being that LabVIEW contains all of the standard methods needed for interfacing to instruments, data acquisition, data analysis, graphics, and also methodology to incorporate programs written in other languages into LabVIEW. Historically, according to Professor Essick, he developed a series of experiments for an upper division laboratory course for computer-based instrumentation. His observation was that while many students had the necessary background in computer programming languages, there were students who had virtually no concept about writing a computer program let alone a computer- based interfacing program. Thus the beginnings of a concept for not only teaching computer- based instrumentation techniques, but aiso a method for the beginner to experience writing a com- puter program. Professor Essick saw LabVIEW as the "perfect environment in which to

Sugarcane smut: shedding light on the development of the whip-shaped sorus

PubMed Central

Marques, João Paulo R.; Appezzato-da-Glória, Beatriz; Piepenbring, Meike; Massola Jr, Nelson S.; Monteiro-Vitorello, Claudia B.

2017-01-01

Background and Aims Sugarcane smut is caused by the fungus Sporisorium scitamineum (Ustilaginales/Ustilaginomycotina/Basidiomycota), which is responsible for losses in sugarcane production worldwide. Infected plants show a profound metabolic modification resulting in the development of a whip-shaped structure (sorus) composed of a mixture of plant tissues and fungal hyphae. Within this structure, ustilospores develop and disseminate the disease. Despite the importance of this disease, a detailed histopathological analysis of the plant–pathogen interaction is lacking. Methods The whip-shaped sorus was investigated using light microscopy, scanning and transmission electron microscopy, histochemical tests and epifluorescence microscopy coupled with deconvolution. Key Results Sorus growth is mediated by intercalary meristem activity at the base of the sorus, where the fungus causes partial host cell wall degradation and formation of intercellular spaces. Sporogenesis in S. scitamineum is thallic, with ustilospore initials in intercalary or terminal positions, and mostly restricted to the base of the sorus. Ustilospore maturation is centrifugal in relation to the ground parenchyma and occurs throughout the sorus median region. At the apex of the sorus, the fungus produces sterile cells and promotes host cell detachment. Hyphae are present throughout the central axis of the sorus (columella). The plant cell produces callose around the intracellular hyphae as well as inside the papillae at the infection site. Conclusions The ontogeny of the whip-shaped sorus suggests that the fungus can cause the acropetal growth in the intercalary meristem. The sporogenesis of S. scitamineum was described in detail, demonstrating that the spores are formed exclusively at the base of the whip. Light was also shed on the nature of the sterile cells. The presence of the fungus alters the host cell wall composition, promotes its degradation and causes the release of some peripheral cells

Fully solution-processed organic light-emitting electrochemical cells (OLEC) with inkjet-printed micro-lenses for disposable lab-on-chip applications at ambient conditions

NASA Astrophysics Data System (ADS)

Shu, Zhe; Pabst, Oliver; Beckert, Erik; Eberhardt, Ramona; Tünnermann, Andreas

2016-02-01

Microfluidic lab-on-chip devices can be used for chemical and biological analyses such as DNA tests or environmental monitoring. Such devices integrate most of the basic functionalities needed for scientific analysis on a microfluidic chip. When using such devices, cost and space-intensive lab equipment is no longer necessary. However, in order to make a monolithic and cost-efficient/disposable microfluidic sensing device, direct integration of the excitation light source for fluorescent sensing is often required. To achieve this, we introduce a fully solution processable deviation of OLEDs, organic light-emitting electrochemical cells (OLECs), as a low-cost excitation light source for a disposable microfluidic sensing platform. By mixing metal ions and a solid electrolyte with light-emitting polymers as active materials, an in-situ doping and in-situ PN-junction can be generated within a three layer sandwich device. Thanks to this doping effect, work function adaptation is not necessary and air-stable electrode can be used. An ambient manufacturing process for fully solution-processed OLECs is presented, which consist of a spin-coated blue light-emitting polymer plus dopants on an ITO cathode and an inkjet-printed PEDOT:PSS transparent top anode. A fully transparent blue OLEC is able to obtain light intensity > 2500 cd/m2 under pulsed driving mode and maintain stable after 1000 cycles, which fulfils requirements for simple fluorescent on-chip sensing applications. However, because of the large refractive index difference between substrates and air, about 80% of emitted light is trapped inside the device. Therefore, inkjet printed micro-lenses on the rear side are introduced here to further increase light-emitting brightness.

Shedding new light on opsin evolution

PubMed Central

Porter, Megan L.; Blasic, Joseph R.; Bok, Michael J.; Cameron, Evan G.; Pringle, Thomas; Cronin, Thomas W.; Robinson, Phyllis R.

2012-01-01

Opsin proteins are essential molecules in mediating the ability of animals to detect and use light for diverse biological functions. Therefore, understanding the evolutionary history of opsins is key to understanding the evolution of light detection and photoreception in animals. As genomic data have appeared and rapidly expanded in quantity, it has become possible to analyse opsins that functionally and histologically are less well characterized, and thus to examine opsin evolution strictly from a genetic perspective. We have incorporated these new data into a large-scale, genome-based analysis of opsin evolution. We use an extensive phylogeny of currently known opsin sequence diversity as a foundation for examining the evolutionary distributions of key functional features within the opsin clade. This new analysis illustrates the lability of opsin protein-expression patterns, site-specific functionality (i.e. counterion position) and G-protein binding interactions. Further, it demonstrates the limitations of current model organisms, and highlights the need for further characterization of many of the opsin sequence groups with unknown function. PMID:22012981

Navigating the gender minefield: An IPV prevention campaign sheds light on the gender gap.

PubMed

Keller, Sarah N; Honea, Joy C

2016-01-01

This article examines how differences in male and female views about intimate partner violence (IPV) contributed to divergent responses to a prevention campaign conducted in the western USA. The study examines focus groups (n = 22) and in-depth interview data (n = 13) collected during campaign development to shed light on quantitative results indicating that women (but not men) increased their perceived severity of domestic violence and awareness of services from pre-test to post-test, while male attitudes moved in the opposite direction. Results of the qualitative study provide the basis for the authors' conclusions about why reactions differed: (1) men's unwillingness to view abuse within a gender context limits men's ability to accept the inequity in statistically demonstrated male and female roles as perpetrators and victims; (2) male resentment of existing gender stereotypes contributed to a rejection of campaign messages that utilised gender prevalence statistics to depict images showing men as perpetrators and women as victims; and (3) victim blaming attitudes contributed to resistance to empathy for victims depicted in the campaign. The authors offer suggestions for future campaigns that foster agency among both perpetrators and survivors while confronting the structural barriers to enacting change.

Seventy Five Years of Particle Accelerators

ScienceCinema

Sessler, Andy

2017-12-09

Andy Sessler, Berkeley Lab director from 1973 to 1980, sheds light on the Lab's nearly eight-decade history of inventing and refining particle accelerators, which continue to illuminate the nature of the universe. His talk was presented July 26, 2006.

Shedding light into the detailed excited-state relaxation pathways and reaction mechanisms of thionaphthol isomers.

PubMed

Riyad, Yasser M; Naumov, Sergej; Hermann, Ralf; Abel, Bernd

2011-02-10

Nanosecond laser flash photolysis employing transient detection of emission and absorption in combination with pulse radiolysis and quantum theory has been employed to shed light into the kinetics, quantum yields, and mechanisms of the deactivation of the first excited singlet state of 1- and 2-thionaphthols (NpSH(S(1))). In contrast to thiophenols (ArSH(S(1))), the results revealed that the decay of the first excited singlet state of 1- and 2-thionaphthols (NpSH(S(1))) is governed by radiationless internal conversion (Φ(IC) = 0.29-0.46; 0.016-0.190) and intersystem crossing (Φ(ISC) = 0.14-0.15; 0.4-0.6), respectively, with pronounced S-H photodissociation (Φ(D) = 0.40-0.55; 0.35-0.40). Fluorescence as a deactivation channel plays a minor role (Φ(F) = 0.001-0.010; 0.010-0.034). Quantum chemical calculations helped in understanding the formation of naphthylthiyl radicals and rationalizing the differences in the efficiency of intersystem crossing of the 1- and 2-thionaphthol systems.

Reflections on Three Corporate Research Labs: Bell Labs, HP Labs, Agilent Labs

NASA Astrophysics Data System (ADS)

Hollenhorst, James

2008-03-01

This will be a personal reflection on corporate life and physics-based research in three industrial research labs over three decades, Bell Labs during the 1980's, HP Labs during the 1990's, and Agilent Labs during the 2000's. These were times of great change in all three companies. I'll point out some of the similarities and differences in corporate cultures and how this impacted the research and development activities. Along the way I'll mention some of the great products that resulted from physics-based R&D.

How counterfactuals of Red-Queen theory shed light on science and its historiography.

PubMed

Dagg, Joachim L

2017-08-01

A historical episode of evolutionary theory, which has lead to the Red Queen theory of the evolutionary maintenance of sex, includes two striking contingencies. These are used to explore alternative what-if scenarios, in order to test some common opinions about such counterfactuals. This sheds new light on the nature of science and its historiography. One counterfactual leads to an unexpected convergence of its result to that of the actual science but, nevertheless, differs in its causal structure. The other diverges towards an incompatible alternative, but this requires further contingent choices that also diverge from actual science. The convergence in the first counterfactual is due to a horizontal transfer of knowledge. Similar transfers of knowledge are typical for innovations of actual science. This suggests that contingent choices can merge as well as fork research traditions both in actual research and counterfactual history. Neither the paths of the actual history of science nor those of its counterfactual alternatives will form a tree of exclusively diverging bifurcations, but a network instead. Convergencies in counterfactuals may, therefore, be due to the web-structure of science as much as to the aims of the historians in question. Furthermore, the difference in causal structure between the actual science and its convergent counterfactual might become diagnostic for external factors rather than internal aims forcing a historian towards convergence. Copyright © 2017 Elsevier Ltd. All rights reserved.

DISTANT VIEW, BLM TACK SHED ON LEFT, BLM SEED SHED ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

DISTANT VIEW, BLM TACK SHED ON LEFT, BLM SEED SHED AT LEFT CENTER, FIRE DISPATCH OFFICES 1 AND 2 AT RIGHT CENTER, UTILITY BUILDING "B" ON RIGHT. VIEW TO SOUTHWEST. - Cedar City Automotive Repair Shop, 820 North Main Street, Cedar City, Iron County, UT

The phenomenon of dynamic stall. [vortex shedding phenomenon on oscillating airfoils

NASA Technical Reports Server (NTRS)

Mccroskey, W. J.

1981-01-01

The general features of dynamic stall on oscillating airfoils are explained in terms of the vortex shedding phenomenon, and the important differences between static stall, light dynamic stall, and deep stall are described. An overview of experimentation and prediction techniques is given.

Reliability and sensitivity of the TonoLab rebound tonometer in awake Brown Norway rats.

PubMed

Morrison, John C; Jia, Lijun; Cepurna, William; Guo, Ying; Johnson, Elaine

2009-06-01

To compare the sensitivity of the TonoLab rebound tonometer with the Tono-Pen in awake Brown Norway rats and to compare their ability to predict optic nerve damage induced by experimental IOP elevation. TonoLab and Tono-Pen tonometers were calibrated in cannulated rat eyes connected to a pressure transducer. The TonoLab was used in awake animals housed in standard lighting to measure IOP during light and dark phases. Both instruments were used to monitor chronically elevated IOP produced by episcleral vein injection of hypertonic saline. Measured IOPs were correlated with quantified optic nerve damage in injected eyes. Although they were lower than transducer and Tono-Pen measurements at all levels, TonoLab readings showed an excellent linear fit with transducer readings from 20 to 80 mm Hg (R(2) = 0.99) in cannulated eyes. In awake animals housed in standard lighting, the TonoLab documented significantly higher pressures during the dark phase (27.9 +/- 1.7 mm Hg) than during the light phase (16.7 +/- 2.3 mm Hg). With elevated IOP, correlation between TonoLab and Tono-Pen readings (R(2) = 0.86, P < 0.0001) was similar to that in cannulated eyes. Although both instruments provided measurements that correlated well with optic nerve injury grade, only the Tono-Pen documented significant IOP elevation in eyes with the least amount of injury (P < 0.05). The TonoLab is sensitive enough to be used in awake Brown Norway rats, though instrument fluctuation may limit its ability to identify significant pressure elevations in eyes with minimal optic nerve damage.

Shedding light on light: benefits of anthropogenic illumination to a nocturnally foraging shorebird.

PubMed

Dwyer, Ross G; Bearhop, Stuart; Campbell, Hamish A; Bryant, David M

2013-03-01

Intertidal habitats provide important feeding areas for migratory shorebirds. Anthropogenic developments along coasts can increase ambient light levels at night across adjacent inter-tidal zones. Here, we report the effects of elevated nocturnal light levels upon the foraging strategy of a migratory shorebird (common redshank Tringa totanus) overwintering on an industrialised estuary in Northern Europe. To monitor behaviour across the full intertidal area, individuals were located by day and night using VHF transmitters, and foraging behaviour was inferred from inbuilt posture sensors. Natural light was scored using moon-phase and cloud cover information and nocturnal artificial light levels were obtained using geo-referenced DMSP/OLS night-time satellite imagery at a 1-km resolution. Under high illumination levels, the commonest and apparently preferred foraging behaviour was sight-based. Conversely, birds feeding in areas with low levels of artificial light had an elevated foraging time and fed by touch, but switched to visual rather than tactile foraging behaviour on bright moonlit nights in the absence of cloud cover. Individuals occupying areas which were illuminated continuously by lighting from a large petrochemical complex invariably exhibited a visually based foraging behaviour independently of lunar phase and cloud cover. We show that ambient light levels affect the timing and distribution of foraging opportunities for redshank. We argue that light emitted from an industrial complex improved nocturnal visibility. This allowed sight-based foraging in place of tactile foraging, implying both a preference for sight-feeding and enhanced night-time foraging opportunities under these conditions. The study highlights the value of integrating remotely sensed data and telemetry techniques to assess the effect of anthropogenic change upon nocturnal behaviour and habitat use. © 2012 The Authors. Journal of Animal Ecology © 2012 British Ecological Society.

Synchrotron sheds new light on geophysical materials

NASA Astrophysics Data System (ADS)

Carlowicz, Michael

On December 20,1996, scientists working with the Advanced Photon Source (APS) at Argonne National Laboratory in Illinois conducted “first light” experiments in a new laboratory for synchrotron radiation research in the geosciences. The demonstration marks the dawn of a new era in rock and mineral physics when, as geophysicist Thomas Duffy of Princeton University notes, researchers will be able to 'shine a bright new light on some of our planet's deepest and darkest secrets.”The new light is from the APS, a particle accelerator dedicated to the production of brilliant X rays for research, and it shone on the GeoSoilEnviroCARS (GSECARS) experimental facility. The purpose of GSECARS is to develop X-ray beamlines at the APS and make them available to scientists for frontier research in Earth, planetary, geophysics, soil, and environmental sciences.

The oldest ionoscopiform from China sheds new light on the early evolution of halecomorph fishes

PubMed Central

Xu, Guang-Hui; Zhao, Li-Jun; Coates, Michael I.

2014-01-01

The Halecomorphi are a major subdivision of the ray-finned fishes. Although living halecomorphs are represented solely by the freshwater bowfin, Amia calva, this clade has a rich fossil history, and the resolution of interrelationships among extinct members is central to the problem of understanding the origin of the Teleostei, the largest clade of extant vertebrates. The Ionoscopiformes are extinct marine halecomorphs that were inferred to have originated in the Late Jurassic of Europe, and subsequently dispersed to the Early Cretaceous of the New World. Here, we report the discovery of a new ionoscopiform, Robustichthys luopingensis gen. et sp. nov., based on eight well-preserved specimens from the Anisian (242–247 Ma), Middle Triassic marine deposits of Luoping, eastern Yunnan Province, China. The new species documents the oldest known ionoscopiform, extending the stratigraphic range of this group by approximately 90 Ma, and the geographical distribution of this group into the Middle Triassic of South China, a part of eastern Palaeotethys Ocean. These new data provide a minimum estimate for the split of Ionoscopiformes from its sister clade Amiiformes and shed new light on the origin of ionoscopiform fishes. PMID:24872460

Circadian Control Sheds Light on Fungal Bioluminescence

PubMed Central

Oliveira, Anderson G.; Stevani, Cassius V.; Waldenmaier, Hans E.; Viviani, Vadim; Emerson, Jillian M.; Loros, Jennifer J.; Dunlap, Jay C.

2015-01-01

Summary Bioluminescence, the creation and emission of light by organisms, affords insight into the lives of organisms doing it. Luminous living things are widespread and access diverse mechanisms to generate and control luminescence [1-5]. Among the least studied bioluminescent organisms are phylogenetically rare fungi – only 71 species, all within the ~9000 fungi of the temperate and tropical Agaricales Order - are reported from among ~100,000 described fungal species [6,7]. All require oxygen [8] and energy (NADH or NADPH) for bioluminescence, and are reported to emit green light (λmax 530 nm) continuously, implying a metabolic function for bioluminescence, perhaps as a by-product of oxidative metabolism in lignin degradation. Here, however, we report that bioluminescence from the mycelium of Neonothopanus gardneri is controlled by a temperature compensated circadian clock, the result of cycles in content/activity of the luciferase, reductase, and the luciferin that comprise the luminescent system. Because regulation implies an adaptive function for bioluminescence, a controversial question for more than two millenia [8-15], we examined interactions between luminescent fungi and insects [16]. Prosthetic acrylic resin “mushrooms”, internally illuminated by a green LED emitting light similar to the bioluminescence, attract staphilinid rove beetles (coleopterans) as well as hemipterans (true bugs), dipterans (flies), and hymenopterans (wasps and ants) at numbers far greater than dark control traps. Thus, circadian control may optimize energy use for when bioluminescence is most visible, attracting insects that can in turn help in spore dispersal, thereby benefitting fungi growing under the forest canopy where wind flow is greatly reduced. PMID:25802150

Tracking dynamics of photoreceptor disc shedding with adaptive optics-optical coherence tomography

NASA Astrophysics Data System (ADS)

Zhang, Furu; Liu, Zhuolin; Kurokawa, Kazuhiro; Miller, Donald T.

2017-02-01

Absorption of light by photoreceptors initiates vision, but also leads to accumulation of toxic photo-oxidative compounds in the photoreceptor outer segment (OS). To prevent this buildup, small packets of OS discs are periodically pruned from the distal end of the OS, a process called disc shedding. Unfortunately dysfunction in any part of the shedding event can lead to photoreceptor and RPE dystrophy, and has been implicated in numerous retinal diseases, including age related macular degeneration and retinitis pigmentosa. While much is known about the complex molecular and signaling pathways that underpin shedding, all of these advancements have occurred in animal models using postmortem eyes. How these translate to the living retina and to humans remain major obstacles. To that end, we have recently discovered the optical signature of cone OS disc shedding in the living human retina, measured noninvasively using optical coherence tomography equipped with adaptive optics in conjunction with post processing methods to track and monitor individual cones in 4D. In this study, we improve on this method in several key areas: increasing image acquisition up to MHz A-scan rates, improving reliability to detect disc shedding events, establishing system precision, and developing cone tracking for use across the entire awake cycle. Thousands of cones were successfully imaged and tracked over the 17 hour period in two healthy subjects. Shedding events were detected in 79.5% and 77.4% of the tracked cones. Similar to previous animal studies, shedding prevalence exhibited a diurnal rhythm. But we were surprised to find that for these two subjects shedding occurred across the entire day with broad, elevated frequency in the morning and decreasing frequency as the day progressed. Consistent with this, traces of the average cone OS length revealed shedding dominated in the morning and afternoon and renewal in the evening.

Longitudinal study of Escherichia coli O157 shedding and super shedding in dairy heifers.

PubMed

Williams, K J; Ward, M P; Dhungyel, O P

2015-04-01

A longitudinal study was conducted to assess the methods available for detection of Escherichia coli O157 and to investigate the prevalence and occurrence of long-term shedding and super shedding in a cohort of Australian dairy heifers. Samples were obtained at approximately weekly intervals from heifers at pasture under normal management systems. Selective sampling techniques were used with the aim of identifying heifers with a higher probability of shedding or super shedding. Rectoanal mucosal swabs (RAMS) and fecal samples were obtained from each heifer. Direct culture of feces was used for detection and enumeration. Feces and RAMS were tested by enrichment culture. Selected samples were further tested retrospectively by immunomagnetic separation of enriched samples. Of 784 samples obtained, 154 (19.6%) were detected as positive using culture methods. Adjusting for selective sampling, the prevalence was 71 (15.6%) of 454. In total, 66 samples were detected as positive at >10(2) CFU/g of which 8 were >10(4) CFU/g and classed as super shedding. A significant difference was observed in detection by enriched culture of RAMS and feces. Dairy heifers within this cohort exhibited variable E. coli O157 shedding, consistent with previous estimates of shedding. Super shedding was detected at a low frequency and inconsistently from individual heifers. All detection methods identified some samples as positive that were not detected by any other method, indicating that the testing methods used will influence survey results.

Shedding Light on the Cosmic Skeleton

NASA Astrophysics Data System (ADS)

2009-11-01

Astronomers have tracked down a gigantic, previously unknown assembly of galaxies located almost seven billion light-years away from us. The discovery, made possible by combining two of the most powerful ground-based telescopes in the world, is the first observation of such a prominent galaxy structure in the distant Universe, providing further insight into the cosmic web and how it formed. "Matter is not distributed uniformly in the Universe," says Masayuki Tanaka from ESO, who led the new study. "In our cosmic vicinity, stars form in galaxies and galaxies usually form groups and clusters of galaxies. The most widely accepted cosmological theories predict that matter also clumps on a larger scale in the so-called 'cosmic web', in which galaxies, embedded in filaments stretching between voids, create a gigantic wispy structure." These filaments are millions of light years long and constitute the skeleton of the Universe: galaxies gather around them, and immense galaxy clusters form at their intersections, lurking like giant spiders waiting for more matter to digest. Scientists are struggling to determine how they swirl into existence. Although massive filamentary structures have been often observed at relatively small distances from us, solid proof of their existence in the more distant Universe has been lacking until now. The team led by Tanaka discovered a large structure around a distant cluster of galaxies in images they obtained earlier. They have now used two major ground-based telescopes to study this structure in greater detail, measuring the distances from Earth of over 150 galaxies, and, hence, obtaining a three-dimensional view of the structure. The spectroscopic observations were performed using the VIMOS instrument on ESO's Very Large Telescope and FOCAS on the Subaru Telescope, operated by the National Astronomical Observatory of Japan. Thanks to these and other observations, the astronomers were able to make a real demographic study of this structure

77 FR 53884 - Automatic Underfrequency Load Shedding and Load Shedding Plans Reliability Standards; Notice of...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-09-04

... Underfrequency Load Shedding and Load Shedding Plans Reliability Standards; Notice of Compliance Filing Take notice that on August 9, 2012, North American Electric Reliability Corporation submitted a compliance... Load Shedding Plans Reliability Standards, 139 FERC ] 61,098, (Order No. 763) (2012). Any person...

The oldest ionoscopiform from China sheds new light on the early evolution of halecomorph fishes.

PubMed

Xu, Guang-Hui; Zhao, Li-Jun; Coates, Michael I

2014-05-01

The Halecomorphi are a major subdivision of the ray-finned fishes. Although living halecomorphs are represented solely by the freshwater bowfin, Amia calva, this clade has a rich fossil history, and the resolution of interrelationships among extinct members is central to the problem of understanding the origin of the Teleostei, the largest clade of extant vertebrates. The Ionoscopiformes are extinct marine halecomorphs that were inferred to have originated in the Late Jurassic of Europe, and subsequently dispersed to the Early Cretaceous of the New World. Here, we report the discovery of a new ionoscopiform, Robustichthys luopingensis gen. et sp. nov., based on eight well-preserved specimens from the Anisian (242-247 Ma), Middle Triassic marine deposits of Luoping, eastern Yunnan Province, China. The new species documents the oldest known ionoscopiform, extending the stratigraphic range of this group by approximately 90 Ma, and the geographical distribution of this group into the Middle Triassic of South China, a part of eastern Palaeotethys Ocean. These new data provide a minimum estimate for the split of Ionoscopiformes from its sister clade Amiiformes and shed new light on the origin of ionoscopiform fishes. © 2014 The Author(s) Published by the Royal Society. All rights reserved.

Adding an extra dimension to what students see through the light microscope: a lab exercise demonstrating critical analysis for microscopy students.

PubMed

Garrill, Ashley

2011-01-01

This article describes an undergraduate lab exercise that demonstrates the importance of students thinking critically about what they see through a microscope. The students are given growth data from tip-growing organisms that suggest the cells grow in a pulsatile manner. The students then critique this data in several exercises that incorporate aspects of a problem-based learning approach, envisaging growth not just in two dimensions, but in three dimensions. For some cells, what appears to be pulsatile growth could also be explained by growth at a constant rate up and down in the z-axis. Depending on the diffraction pattern generated by the tip of the cell, this movement in the z-axis could go undetected. This raises the possibility that pulsatile growth seen in some species may be an artifact generated by the limitations of the light microscope. Students were subsequently asked to rate their awareness of the need to think critically about what they see through a microscope, using a scale of 1 (unaware) to 5 (very much aware). Prior to doing the lab exercise, the mean rating was 2.7; this increased to 4.4 after the lab. The students also indicated a likelihood of being more critical in their thinking in other aspects of their biology curriculum.

Risk factors for Escherichia coli O157 shedding and super-shedding by dairy heifers at pasture.

PubMed

Williams, K J; Ward, M P; Dhungyel, O P; Hall, E J S

2015-04-01

We undertook a longitudinal study within a cohort of 52 dairy heifers maintained under constant management systems and sampled weekly to investigate a comprehensive range of risk factors which may influence shedding or super-shedding of E. coli O157 (detected by direct faecal culture and immunomagnetic separation). E. coli O157 was detected from 416/933 (44.6%) samples (faeces and recto-anal mucosal swabs) and 32 (3.4%) samples enumerated at >10000 c.f.u./g. Weekly point prevalence ranged from 9.4% to 94.3%. Higher temperature (P < 0.001), rainfall (P = 0.02), relative humidity (P < 0.001), pasture growth (P = 0.013) and body score (P = 0.029) were positively associated with increased shedding. Higher rainfall (P < 0.001), hide contamination (P = 0.002) and increased faecal consistency (P = 0.023) were positively associated with super-shedding. Increased solar exposure had a negative effect on both shedding and super-shedding within bivariate analyses but in the final multivariate model for shedding demonstrated a positive effect (P = 0.017). Results suggest that environmental factors are important in E. coli O157 shedding in cattle.

Replication, pathogenicity, shedding, and transmission of Zaire ebolavirus in pigs.

PubMed

Kobinger, Gary P; Leung, Anders; Neufeld, James; Richardson, Jason S; Falzarano, Darryl; Smith, Greg; Tierney, Kevin; Patel, Ami; Weingartl, Hana M

2011-07-15

(See the editorial commentary by Bausch, on pages 179-81.) Reston ebolavirus was recently detected in pigs in the Philippines. Specific antibodies were found in pig farmers, indicating exposure to the virus. This important observation raises the possibility that pigs may be susceptible to Ebola virus infection, including from other species, such as Zaire ebolavirus (ZEBOV), and can transmit to other susceptible hosts. This study investigated whether ZEBOV, a species commonly reemerging in central Africa, can replicate and induce disease in pigs and can be transmitted to naive animals. Domesticated Landrace pigs were challenged through mucosal exposure with a total of 1 ×10(6) plaque-forming units of ZEBOV and monitored for virus replication, shedding, and pathogenesis. Using similar conditions, virus transmission from infected to naive animals was evaluated in a second set of pigs. Following mucosal exposure, pigs replicated ZEBOV to high titers (reaching 10(7) median tissue culture infective doses/mL), mainly in the respiratory tract, and developed severe lung pathology. Shedding from the oronasal mucosa was detected for up to 14 days after infection, and transmission was confirmed in all naive pigs cohabiting with inoculated animals. These results shed light on the susceptibility of pigs to ZEBOV infection and identify an unexpected site of virus amplification and shedding linked to transmission of infectious virus.

Micromixer utilizing electrokinetic instability-induced shedding effect.

PubMed

Tai, Chang-Hsien; Yang, Ruey-Jen; Huang, Min-Zhong; Liu, Chia-Wei; Tsai, Chien-Hsiung; Fu, Lung-Ming

2006-12-01

This paper presents a T-shaped micromixer featuring 45 degrees parallelogram barriers (PBs) within the mixing channel. The presented device obtains a rapid mixing of two sample fluids with conductivity ratio of 10:1 (sample concentration:running buffer concentration) by means of the electrokinetic instability-induced shedding effects which are produced when a direct current (DC) electric field of an appropriate intensity is applied. The presented device uses a single high-voltage power source to simultaneously drive and mix the sample fluids. The effectiveness of the mixer is characterized experimentally as a function of the applied electrical field intensity and the extent to which the PBs obstruct the mixing channel. The experimental results indicate that the mixing performance reaches 91% at a cross-section located 2.3 mm downstream of the T-junction when the barriers obstruct 4/5 of the channel width and an electrical field of 300 V/cm is applied. The micromixing method presented in this study provides a simple low-cost solution to mixing problems in lab-on-a-chip systems.

Quantification of eDNA shedding rates from invasive bighead carp Hypophthalmichthys nobilis and silver carp Hypophthalmichthys molitrix

USGS Publications Warehouse

Klymus, Katy E.; Richter, Catherine A.; Chapman, Duane C.; Paukert, Craig P.

2015-01-01

Wildlife managers can more easily mitigate the effects of invasive species if action takes place before a population becomes established. Such early detection requires sensitive survey tools that can detect low numbers of individuals. Due to their high sensitivity, environmental DNA (eDNA) surveys hold promise as an early detection method for aquatic invasive species. Quantification of eDNA amounts may also provide data on species abundance and timing of an organism’s presence, allowing managers to successfully combat the spread of ecologically damaging species. To better understand the link between eDNA and an organism’s presence, it is crucial to know how eDNA is shed into the environment. Our study used quantitative PCR (qPCR) and controlled laboratory experiments to measure the amount of eDNA that two species of invasive bigheaded carps (Hypophthalmichthys nobilis and Hypophthalmichthys molitrix) shed into the water. We first measured how much eDNA a single fish sheds and the variability of these measurements. Then, in a series of manipulative lab experiments, we studied how temperature, biomass (grams of fish), and diet affect the shedding rate of eDNA by these fish. We found that eDNA amounts exhibit a positive relationship with fish biomass, and that feeding could increase the amount of eDNA shed by ten-fold, whereas water temperature did not have an effect. Our results demonstrate that quantification of eDNA may be useful for predicting carp density, as well as densities of other rare or invasive species.

Shedding light on moths: shorter wavelengths attract noctuids more than geometrids

PubMed Central

Somers-Yeates, Robin; Hodgson, David; McGregor, Peter K.; Spalding, Adrian; ffrench-Constant, Richard H.

2013-01-01

With moth declines reported across Europe, and parallel changes in the amount and spectra of street lighting, it is important to understand exactly how artificial lights affect moth populations. We therefore compared the relative attractiveness of shorter wavelength (SW) and longer wavelength (LW) lighting to macromoths. SW light attracted significantly more individuals and species of moth, either when used alone or in competition with LW lighting. We also found striking differences in the relative attractiveness of different wavelengths to different moth groups. SW lighting attracted significantly more Noctuidae than LW, whereas both wavelengths were equally attractive to Geometridae. Understanding the extent to which different groups of moth are attracted to different wavelengths of light will be useful in determining the impact of artificial light on moth populations. PMID:23720524

Oxygen quenching in a LAB based liquid scintillator and the nitrogen bubbling model

NASA Astrophysics Data System (ADS)

Xiao, Hua-Lin; Deng, Jing-Shan; Wang, Nai-Yan

2010-05-01

The oxygen quenching effect in a Linear Alkl Benzene (LAB) based liquid scintillator (LAB as the solvent, 3 g/L 2, 5 diphe-nyloxazole (PPO) as the fluor and 15 mg/L p-bis-(o-methylstyryl)-benzene (bis-MSB) as the λ-shifter) is studied by measuring the light yield as a function of the nitrogen bubbling time. It is shown that the light yield of the fully purged liquid scintillator is increased by 11% at room temperature and the room atmospheric pressure. A simple nitrogen bubbling model is proposed to describe the relationship between the relative light yield (oxygen quenching factor) and the bubbling time.

Cherenkov and scintillation light separation in organic liquid scintillators

NASA Astrophysics Data System (ADS)

Caravaca, J.; Descamps, F. B.; Land, B. J.; Yeh, M.; Orebi Gann, G. D.

2017-12-01

The CHErenkov/Scintillation Separation experiment (CHESS) has been used to demonstrate the separation of Cherenkov and scintillation light in both linear alkylbenzene (LAB) and LAB with 2 g/L of PPO as a fluor (LAB/PPO). This is the first successful demonstration of Cherenkov light detection from the more challenging LAB/PPO cocktail and improves on previous results for LAB. A time resolution of 338± 12 ps FWHM results in an efficiency for identifying Cherenkov photons in LAB/PPO of 70 ± 3 % and 63± 8% for time- and charge-based separation, respectively, with scintillation contamination of 36± 5% and 38± 4%. LAB/PPO data is consistent with a rise time of τ _r=0.72± 0.33 ns.

Coxiella burnetii shedding by dairy cows.

PubMed

Guatteo, Raphaël; Beaudeau, François; Joly, Alain; Seegers, Henri

2007-01-01

While shedding routes of Coxiella burnetii are identified, the characteristics of Coxiella shedding are still widely unknown, especially in dairy cattle. However, this information is crucial to assess the natural course of Coxiella burnetii infection within a herd and then to elaborate strategies to limit the risks of transmission between animals and to humans. The present study aimed at (i) describing the characteristics of Coxiella burnetii shedding by dairy cows (in milk, vaginal mucus, faeces) in five infected dairy herds, and at (ii) investigating the possible relationships between shedding patterns and serological responses. A total of 145 cows were included in a follow-up consisting of seven concomitant samplings of milk, vaginal mucus, faeces and blood (Day 0, D7, D14, D21, D28, D63, D90). Detection and quantification of Coxiella burnetii titres were performed in milk, vaginal mucus and faeces samples using real-time PCR assay, while antibodies against Coxiella were detected using an ELISA technique. For a given shedding route, and a given periodicity (weekly or monthly), cows were gathered into different shedding kinetic patterns according to the sequence of PCR responses. Distribution of estimated titres in Coxiella burnetii was described according to shedding kinetic patterns. Coxiella burnetii shedding was found scarcely and sporadically in faeces. Vaginal mucus shedding concerned almost 50% of the cows studied and was found intermittently or sporadically, depending on the periodicity considered. Almost 40% of cows were detected as milk shedders, with two predominant shedding patterns: persistent and sporadic, regardless of the sampling periodicity. Significantly higher estimated titres in Coxiella burnetii were observed in cows with persistent shedding patterns suggesting the existence of heavy shedder cows. These latter cows were mostly, persistently highly-seropositive, suggesting that repeated serological testings could be a reliable tool to screen

Berkeley Lab - Lawrence Berkeley National Laboratory

Science.gov Websites

nanoparticles that could make solar panels more efficient by converting light usually missed by solar cells into of Methane's Increasing Greenhouse Effect A Berkeley Lab research team tracked a rise in the warming effect of methane - one of the most important greenhouse gases for the Earth's atmosphere - over a 10

StagLab: Post-Processing and Visualisation in Geodynamics

NASA Astrophysics Data System (ADS)

Crameri, Fabio

2017-04-01

Despite being simplifications of nature, today's Geodynamic numerical models can, often do, and sometimes have to become very complex. Additionally, a steadily-increasing amount of raw model data results from more elaborate numerical codes and the still continuously-increasing computational power available for their execution. The current need for efficient post-processing and sensible visualisation is thus apparent. StagLab (www.fabiocrameri.ch/software) provides such much-needed strongly-automated post-processing in combination with state-of-the-art visualisation. Written in MatLab, StagLab is simple, flexible, efficient and reliable. It produces figures and movies that are both fully-reproducible and publication-ready. StagLab's post-processing capabilities include numerous diagnostics for plate tectonics and mantle dynamics. Featured are accurate plate-boundary identification, slab-polarity recognition, plate-bending derivation, mantle-plume detection, and surface-topography component splitting. These and many other diagnostics are derived conveniently from only a few parameter fields thanks to powerful image processing tools and other capable algorithms. Additionally, StagLab aims to prevent scientific visualisation pitfalls that are, unfortunately, still too common in the Geodynamics community. Misinterpretation of raw data and exclusion of colourblind people introduced with the continuous use of the rainbow (a.k.a. jet) colour scheme is just one, but a dramatic example (e.g., Rogowitz and Treinish, 1998; Light and Bartlein, 2004; Borland and Ii, 2007). StagLab is currently optimised for binary StagYY output (e.g., Tackley 2008), but is adjustable for the potential use with other Geodynamic codes. Additionally, StagLab's post-processing routines are open-source. REFERENCES Borland, D., and R. M. T. Ii (2007), Rainbow color map (still) considered harmful, IEEE Computer Graphics and Applications, 27(2), 14-17. Light, A., and P. J. Bartlein (2004), The end of

Pair spectrometer hodoscope for Hall D at Jefferson Lab

DOE PAGES

Barbosa, Fernando J.; Hutton, Charles L.; Sitnikov, Alexandre; ...

2015-09-21

We present the design of the pair spectrometer hodoscope fabricated at Jefferson Lab and installed in the experimental Hall D. The hodoscope consists of thin scintillator tiles; the light from each tile is collected using wave-length shifting fibers and detected using a Hamamatsu silicon photomultiplier. Light collection was measured using relativistic electrons produced in the tagger area of the experimental Hall B.

Pair spectrometer hodoscope for Hall D at Jefferson Lab

DOE Office of Scientific and Technical Information (OSTI.GOV)

Barbosa, Fernando J.; Hutton, Charles L.; Sitnikov, Alexandre

We present the design of the pair spectrometer hodoscope fabricated at Jefferson Lab and installed in the experimental Hall D. The hodoscope consists of thin scintillator tiles; the light from each tile is collected using wave-length shifting fibers and detected using a Hamamatsu silicon photomultiplier. Light collection was measured using relativistic electrons produced in the tagger area of the experimental Hall B.

Shedding new light on viral photosynthesis.

PubMed

Puxty, Richard J; Millard, Andrew D; Evans, David J; Scanlan, David J

2015-10-01

Viruses infecting the environmentally important marine cyanobacteria Prochlorococcus and Synechococcus encode 'auxiliary metabolic genes' (AMGs) involved in the light and dark reactions of photosynthesis. Here, we discuss progress on the inventory of such AMGs in the ever-increasing number of viral genome sequences as well as in metagenomic datasets. We contextualise these gene acquisitions with reference to a hypothesised fitness gain to the phage. We also report new evidence with regard to the sequence and predicted structural properties of viral petE genes encoding the soluble electron carrier plastocyanin. Viral copies of PetE exhibit extensive modifications to the N-terminal signal peptide and possess several novel residues in a region responsible for interaction with redox partners. We also highlight potential knowledge gaps in this field and discuss future opportunities to discover novel phage-host interactions involved in the photosynthetic process.

Lipid shedding from single oscillating microbubbles.

PubMed

Luan, Ying; Lajoinie, Guillaume; Gelderblom, Erik; Skachkov, Ilya; van der Steen, Antonius F W; Vos, Hendrik J; Versluis, Michel; De Jong, Nico

2014-08-01

Lipid-coated microbubbles are used clinically as contrast agents for ultrasound imaging and are being developed for a variety of therapeutic applications. The lipid encapsulation and shedding of the lipids by acoustic driving of the microbubble has a crucial role in microbubble stability and in ultrasound-triggered drug delivery; however, little is known about the dynamics of lipid shedding under ultrasound excitation. Here we describe a study that optically characterized the lipid shedding behavior of individual microbubbles on a time scale of nanoseconds to microseconds. A single ultrasound burst of 20 to 1000 cycles, with a frequency of 1 MHz and an acoustic pressure varying from 50 to 425 kPa, was applied. In the first step, high-speed fluorescence imaging was performed at 150,000 frames per second to capture the instantaneous dynamics of lipid shedding. Lipid detachment was observed within the first few cycles of ultrasound. Subsequently, the detached lipids were transported by the surrounding flow field, either parallel to the focal plane (in-plane shedding) or in a trajectory perpendicular to the focal plane (out-of-plane shedding). In the second step, the onset of lipid shedding was studied as a function of the acoustic driving parameters, for example, pressure, number of cycles, bubble size and oscillation amplitude. The latter was recorded with an ultrafast framing camera running at 10 million frames per second. A threshold for lipid shedding under ultrasound excitation was found for a relative bubble oscillation amplitude >30%. Lipid shedding was found to be reproducible, indicating that the shedding event can be controlled. Copyright © 2014 World Federation for Ultrasound in Medicine & Biology. Published by Elsevier Inc. All rights reserved.

UVC Sheds New Light on School Mold Problems.

ERIC Educational Resources Information Center

Freeman, James

2002-01-01

Describes how the LaPorte Independent School District in Texas turned to ultraviolent light devices installed within the HVAC system to combat mold and fungus after conventional approaches failed. Describes the additional benefits of energy savings from the system. (EV)

Cherenkov and scintillation light separation in organic liquid scintillators

DOE PAGES

Caravaca, J.; Descamps, F. B.; Land, B. J.; ...

2017-11-29

The CHErenkov/Scintillation Separation experiment (CHESS) has been used to demonstrate the separation of Cherenkov and scintillation light in both linear alkylbenzene (LAB) and LAB with 2 g/L of PPO as a fluor (LAB/PPO). This is the first successful demonstration of Cherenkov light detection from the more challenging LAB/PPO cocktail and improves on previous results for LAB. A time resolution of 338 ± 12 ps FWHM results in an efficiency for identifying Cherenkov photons in LAB/PPO of 70 ± 3 % and 63 ± 8 % for time- and charge-based separation, respectively, with scintillation contamination of 36 ± 5 % andmore » 38 ± 4 %. LAB/PPO data is consistent with a rise time of τ r = 0.72 ± 0.33 ns.« less

Cherenkov and scintillation light separation in organic liquid scintillators

DOE Office of Scientific and Technical Information (OSTI.GOV)

Caravaca, J.; Descamps, F. B.; Land, B. J.

The CHErenkov/Scintillation Separation experiment (CHESS) has been used to demonstrate the separation of Cherenkov and scintillation light in both linear alkylbenzene (LAB) and LAB with 2 g/L of PPO as a fluor (LAB/PPO). This is the first successful demonstration of Cherenkov light detection from the more challenging LAB/PPO cocktail and improves on previous results for LAB. A time resolution of 338 ± 12 ps FWHM results in an efficiency for identifying Cherenkov photons in LAB/PPO of 70 ± 3 % and 63 ± 8 % for time- and charge-based separation, respectively, with scintillation contamination of 36 ± 5 % andmore » 38 ± 4 %. LAB/PPO data is consistent with a rise time of τ r = 0.72 ± 0.33 ns.« less

Love the Lab, Hate the Lab Report?

ERIC Educational Resources Information Center

Bjorn, Genevive

2018-01-01

In the author's large, urban high school, enrollment in a laboratory science is mandatory. While the student participation rate for lab activities is over 98%, the turn-in rate for traditional lab reports averages just 35% to 85%. Those students who don't produce a lab report miss a critical opportunity to improve their skills in scientific…

SHEDS-Dietary Technical Manual Appendices

EPA Pesticide Factsheets

The appendices for the SHEDS-Dietary Technical Manual include a sample food diary, backgorund information on the water concentration data used in SHEDS-Dietary, a food list, food definitions and sample code.

Lab Simulates Outdoor Algae Growth

DOE Office of Scientific and Technical Information (OSTI.GOV)

None

Algae can be turned into renewable biofuel, which is why scientists want to discover an inexpensive, fast-growing strain of algae. Scientists at Pacific Northwest National Laboratory have developed a system to speed up this search. The unique climate-simulating system uses temperature controls and multi-colored LED lights to mimic the constantly changing conditions of an outdoor algae pond. By simulating outdoor climates inside the lab, the system saves researchers time and expense.

Shedding Light on Light Pollution: Reports from GLOBE at Night

NASA Astrophysics Data System (ADS)

Walker, Constance E.; Pompea, S. M.; Isbell, D.

2009-05-01

The citizen-science program on light pollution, GLOBE at Night, has had rich responses during this year's campaign in March 2009. Reporting on some of the highlights, we will hear success stories and lessons learned from educators, students, science centers and astronomy clubs from around the world. Communities will be featured from several cities, such Norman, Oklahoma, Mishawaka, Indiana, Willimantic, Connecticut, and Waynesville, Ohio, which created mini-campaigns that combined local students with public advocates and representatives from local city and county governments. Connecticut kids collaborated with students in Wales, Canada and Romania on GLOBE at Night, and an extensive campaign was planned with the schools near the observatories of north-central Chile. Groups that have received special training in GLOBE at Night and related activities include the "Astronomy from the Ground Up” network of science and nature centers (fostered by the ASP and the NSF), 146 amateur astronomers who are part of the ASP-NASA Night-Sky Network, and the Association of Science-Technology Centers. Special training was given over forums, telecon-powerpoint presentations and blogs, to fit the needs of the communities. Among the more interesting media efforts for the general public, GLOBE at Night was the topic of the March 6 episode of the IYA2009 "Days of Astronomy" podcast. International organizing efforts for GLOBE at Night have been strong in countries like Argentina, Australia, Austria, Brazil, Canada, Chile, Costa Rica, the Czech Republic, Germany, Hungary, Ireland, Poland, Portugal, Romania, Spain and the United Kingdom, to name a few. We will also discuss how cities, such as Tucson, Arizona, combined efforts on GLOBE at Night with involvement in the World Wildlife Fund's Earth Hour event (www.earthhour.org). Earth Hour encouraged everyone to turn out their lights from 8:30-9:30 p.m. local time on Saturday, March 28, the final night of GLOBE at Night 2009.

Pulse Oximetry in the Physics Lab: A Colorful Alternative to Traditional Optics Curricula

ERIC Educational Resources Information Center

Kutschera, Ellynne; Dunlap, Justin C.; Byrd, Misti; Norlin, Casey; Widenhorn, Ralf

2013-01-01

We designed a physics laboratory exercise around pulse oximetry, a noninvasive medical technique used to assess a patient's blood oxygen saturation. An alternative to a traditional optics and light lab, this exercise teaches the principles of light absorption, spectroscopy, and the properties of light, while simultaneously studying a common…

Shear driven droplet shedding and coalescence on a superhydrophobic surface

NASA Astrophysics Data System (ADS)

Moghtadernejad, S.; Tembely, M.; Jadidi, M.; Esmail, N.; Dolatabadi, A.

2015-03-01

The interest on shedding and coalescence of sessile droplets arises from the importance of these phenomena in various scientific problems and industrial applications such as ice formation on wind turbine blades, power lines, nacelles, and aircraft wings. It is shown recently that one of the ways to reduce the probability of ice accretion on industrial components is using superhydrophobic coatings due to their low adhesion to water droplets. In this study, a combined experimental and numerical approach is used to investigate droplet shedding and coalescence phenomena under the influence of air shear flow on a superhydrophobic surface. Droplets with a size of 2 mm are subjected to various air speeds ranging from 5 to 90 m/s. A numerical simulation based on the Volume of Fluid method coupled with the Large Eddy Simulation turbulent model is carried out in conjunction with the validating experiments to shed more light on the coalescence of droplets and detachment phenomena through a detailed analysis of the aerodynamics forces and velocity vectors on the droplet and the streamlines around it. The results indicate a contrast in the mechanism of two-droplet coalescence and subsequent detachment with those related to the case of a single droplet shedding. At lower speeds, the two droplets coalesce by attracting each other with successive rebounds of the merged droplet on the substrate, while at higher speeds, the detachment occurs almost instantly after coalescence, with a detachment time decreasing exponentially with the air speed. It is shown that coalescence phenomenon assists droplet detachment from the superhydrophobic substrate at lower air speeds.

Germany plans 60m euro physics and medicine lab

NASA Astrophysics Data System (ADS)

Stafford, Ned

2017-09-01

A new €60m medical-physics research lab is to be built in Erlangen, Germany, by the Max Planck Institute for the Science of Light (MPL) together with the Friedrich Alexander University Erlangen-Nürnberg and the University Hospital Erlangen.

Alberi Validates New Theory, Sheds Light on Semiconductors | News | NREL

Science.gov Websites

the discovery when they found that light can suppress native defect formation during semiconductor growth. When Alberi and Scarpulla began discussing the concept of how light can affect semiconductor that pieces easily fit together, so are the atoms in the crystal. But when an atom appears in a crystal

Shedding (Incoherent) Light on Quantum Effects in Light-Induced Biological Processes.

PubMed

Brumer, Paul

2018-05-18

Light-induced processes that occur in nature, such as photosynthesis and photoisomerization in the first steps in vision, are often studied in the laboratory using coherent pulsed laser sources, which induce time-dependent coherent wavepacket molecule dynamics. Nature, however, uses stationary incoherent thermal radiation, such as sunlight, leading to a totally different molecular response, the time-independent steady state. It is vital to appreciate this difference in order to assess the role of quantum coherence effects in biological systems. Developments in this area are discussed in detail.

This pineal gland does not mediate phase shifts in the disc shedding rhythm of the rat retina

DOE Office of Scientific and Technical Information (OSTI.GOV)

Goldman, A.I.

Albino rats were subjected to pinealectomy, superior cervical ganglionectomy, or the appropriate sham preparation and were placed in lighting conditions so that light onset was advanced by 10 hr. After 6 days of this regimen, all animals exhibited a complete shift in their outer segment disc shedding rhythm, indicating that the pineal gland is not a factor in mediating such a shift.

Optical imaging of architecture and function in the living brain sheds new light on cortical mechanisms underlying visual perception.

PubMed

Grinvald, A

1992-01-01

Long standing questions related to brain mechanisms underlying perception can finally be resolved by direct visualization of the architecture and function of mammalian cortex. This advance has been accomplished with the aid of two optical imaging techniques with which one can literally see how the brain functions. The upbringing of this technology required a multi-disciplinary approach integrating brain research with organic chemistry, spectroscopy, biophysics, computer sciences, optics and image processing. Beyond the technological ramifications, recent research shed new light on cortical mechanisms underlying sensory perception. Clinical applications of this technology for precise mapping of the cortical surface of patients during neurosurgery have begun. Below is a brief summary of our own research and a description of the technical specifications of the two optical imaging techniques. Like every technique, optical imaging also suffers from severe limitations. Here we mostly emphasize some of its advantages relative to all alternative imaging techniques currently in use. The limitations are critically discussed in our recent reviews. For a series of other reviews, see Cohen (1989).

Evaluation of Light Collection System for Pion and Kaon Experiments in Hall C at Jefferson Lab

NASA Astrophysics Data System (ADS)

Roustom, Salim

2017-09-01

The neutral pion and the kaon are opportune to study the hadron structure through General Parton Distributions, which can be viewed as spatial densities at different momenta of the quarks inside the proton. To study hadron structure with pion or kaon experiments in Hall C at 12 GeV Jefferson Lab, one must analyze the final state neutral pions and kaons and their decay products. For the analysis of these particles, dedicated detectors based on the Cherenkov or scintillation mechanism are used, e.g. the HMS and SHMS aerogel detectors and the PbWO4-based Neutral Particle Spectrometer. A critical part of these detectors is the light collection system. Photomultiplier Tubes (PMTs) have many advantages, however, they are sensitive to magnetic fields and can get damaged by elevated helium levels in the atmosphere. An alternative to PMTs are Avalanche Photodiodes (APDs). APDs are sensitive to background noise, temperature, and radiation. It is thus important to evaluate the benefits of each light collection system and optimize operating conditions to ensure performance over a reasonably long time. I will present a performance study of PMTs exposed to elevated levels of helium and a comparison of APDs as alternatives, as well as new, compact readout methods. Supported in part by NSF Grants PHY-1714133, PHY-1530874, PHY-1306227 and PHY-1306418.

Jonathan F. Reichert and Barbara Wolff-Reichert Award for Excellence in Advanced Laboratory Instruction: Advanced Instructional Labs: Why Bother?

NASA Astrophysics Data System (ADS)

Bistrow, Van

What aren't we teaching about physics in the traditional lecture course? Plenty! By offering the Advanced Laboratory Course, we hope to shed light on the following questions: How do we develop a systematic process of doing experiments? How do we record procedures and results? How should we interpret theoretical concepts in the real world? What experimental and computational techniques are available for producing and analyzing data? With what degree of confidence can we trust our measurements and interpretations? How well does a theory represent physical reality? How do we collaborate with experimental partners? How do we best communicate our findings to others?These questions are of fundamental importance to experimental physics, yet are not generally addressed by reading textbooks, attending lectures or doing homework problems. Thus, to provide a more complete understanding of physics, we offer laboratory exercises as a supplement to the other modes of learning. The speaker will describe some examples of experiments, and outline the history, structure and student impressions of the Advanced Lab course at the University of Chicago Department of Physics.

Optics and optics-based technologies education with the benefit of LabVIEW

NASA Astrophysics Data System (ADS)

Wan, Yuhong; Man, Tianlong; Tao, Shiquan

2015-10-01

The details of design and implementation of incoherent digital holographic experiments based on LabVIEW are demonstrated in this work in order to offer a teaching modal by making full use of LabVIEW as an educational tool. Digital incoherent holography enables holograms to be recorded from incoherent light with just a digital camera and spatial light modulator and three-dimensional properties of the specimen are revealed after the hologram is reconstructed in the computer. The experiment of phase shifting incoherent digital holography is designed and implemented based on the principle of Fresnel incoherent correlation holography. An automatic control application is developed based on LabVIEW, which combines the functions of major experimental hardware control and digital reconstruction of the holograms. The basic functions of the system are completed and a user-friendly interface is provided for easy operation. The students are encouraged and stimulated to learn and practice the basic principle of incoherent digital holography and other related optics-based technologies during the programming of the application and implementation of the system.

Hair shedding score may affect body temperature more than hair coat color during heat stress in weaned beef heifers.

USDA-ARS?s Scientific Manuscript database

The objective of this study was to evaluate the effect of hair shedding score and hair coat color on the vaginal temperature (VT) of calves during heat stress. Weaned Bos taurus beef heifers (n = 32; BW = 282 ± 6.4 kg) were assigned to a hair coat color class (BLACK; RED; or LIGHT, where LIGHT = yel...

Measurement of α -particle quenching in LAB based scintillator in independent small-scale experiments

DOE Office of Scientific and Technical Information (OSTI.GOV)

von Krosigk, B.; Chen, M.; Hans, S.

2016-02-29

The α -particle light response of liquid scintillators based on linear alkylbenzene (LAB) has been measured with three different experimental approaches. In the first approach, α -particles were produced in the scintillator via 12C(n,α) 9 Be reactions. In the second approach, the scintillator was loaded with 2 % of natSm providing an α-emitter, 147Sm, as an internal source. In the third approach, a scintillator flask was deployed into the water-filled SNO+ detector and the radioactive contaminants 222Rn, 218Po and 214Po provided the α -particle signal. The behavior of the observed α -particle light outputs are in agreement with each casemore » successfully described by Birks’ law. The resulting Birks parameter kB ranges from (0.0066±0.0016) to (0.0076±0.0003) cm/MeV. In the first approach, the α -particle light response was measured simultaneously with the light response of recoil protons produced via neutron–proton elastic scattering. This enabled a first time a direct comparison of kB describing the proton and the α -particle response of LAB based scintillator. The observed kB values describing the two light response functions deviate by more than 5σ . The presented results are valuable for all current and future detectors, using LAB based scintillator as target, since they depend on an accurate knowledge of the scintillator response to different particles.« less

Berkeley Lab Training

Science.gov Websites

Berkeley Lab Berkeley Lab A-Z Index Phone Book Jobs Search DOE Help Berkeley Lab Training Welcome Welcome to Berkeley Lab Training! Login to access your LBNL Training Profile. This provides quick access to all of the courses you need. Look below, to learn about different types of training available at

Light Activated Escape Circuits: A Behavior and Neurophysiology Lab Module using Drosophila Optogenetics

PubMed Central

Titlow, Josh S.; Johnson, Bruce R.; Pulver, Stefan R.

2015-01-01

The neural networks that control escape from predators often show very clear relationships between defined sensory inputs and stereotyped motor outputs. This feature provides unique opportunities for researchers, but it also provides novel opportunities for neuroscience educators. Here we introduce new teaching modules using adult Drosophila that have been engineered to express csChrimson, a red-light sensitive channelrhodopsin, in specific sets of neurons and muscles mediating visually guided escape behaviors. This lab module consists of both behavior and electrophysiology experiments that explore the neural basis of flight escape. Three preparations are described that demonstrate photo-activation of the giant fiber circuit and how to quantify these behaviors. One of the preparations is then used to acquire intracellular electrophysiology recordings from different flight muscles. The diversity of action potential waveforms and firing frequencies observed in the flight muscles make this a rich preparation to study the ionic basic of cellular excitability. By activating different cells within the giant fiber pathway we also demonstrate principles of synaptic transmission and neural circuits. Beyond conveying core neurobiological concepts it is also expected that using these cutting edge techniques will enhance student motivation and attitudes towards biological research. Data collected from students and educators who have been involved in development of the module are presented to support this notion. PMID:26240526

The distribution of actin immunoreactivity in rhabdomeres of tipulid flies in relation to extracellular membrane shedding.

PubMed

Blest, A D; Stowe, S; Clausen, J A; Carter, M

1991-09-01

Rhabdomeres of tipulid flies lose membrane during turnover from a 'shedding zone' composed of microvillar tips. These distal domains lack intramicrovillar cytoskeletons and appear to be empty sacs of membrane. Recent concerns about the role of ninaC mechano-enzymes in the architecture of dipteran rhabdomeral microvilli and the dynamic role that they may play in the creation of shedding zones demand an examination of the distribution of actin in tipulid rhabdomeres. We compared rhabdomeres from tipulid retinae incubated before fixation for immunocytochemistry in a buffer without additives and a stabilising buffer that contained a cocktail of cysteine protease inhibitors; both were challenged by an anti-actin antibody for immunogold labelling after embedding in LR White Resin. Shedding zones thus processed collapse to structureless detritus. Stabilised and unstabilized shedding zones were immunonegative to anti-actin. To ensure that the negative results were not consequent upon conformational changes generated by the processing protocol, we examined microvilli of degenerating rhabdomeres of the Drosophila light-dependent retinal degeneration mutant rdgBKS222 (which separate and collapse without creating a shedding zone) and found the detritus they generate to be immunopositive to anti-actin. Stabilised and unstabilized regions of basal regions of tipulid rhabdomeres were equally immunopositive. We infer that (a) actin is absent from shedding zones; (b) actin is not degraded by microvillar cysteine proteases. The implications of these conclusions are discussed in relation to some functional models of arthropod photoreceptor microvilli.

Hundreds of Area Residents Visit the National Lab Booth at the Annual In The Street Festival | Poster

Cancer.gov

Light-up yo-yos, brightly colored portion plates, and a fast spinner game lured hundreds of area residents to the Frederick National Lab booth at this year’s In The Street festival, where they also heard a message from the lab: Stay healthy through healthy habits.

Lab-on-fiber technology: a new vision for chemical and biological sensing.

PubMed

Ricciardi, Armando; Crescitelli, Alessio; Vaiano, Patrizio; Quero, Giuseppe; Consales, Marco; Pisco, Marco; Esposito, Emanuela; Cusano, Andrea

2015-12-21

The integration of microfluidics and photonic biosensors has allowed achievement of several laboratory functions in a single chip, leading to the development of photonic lab-on-a-chip technology. Although a lot of progress has been made to implement such sensors in small and easy-to-use systems, many applications such as point-of-care diagnostics and in vivo biosensing still require a sensor probe able to perform measurements at precise locations that are often hard to reach. The intrinsic property of optical fibers to conduct light to a remote location makes them an ideal platform to meet this demand. The motivation to combine the good performance of photonic biosensors on chips with the unique advantages of optical fibers has thus led to the development of the so-called lab-on-fiber technology. This emerging technology envisages the integration of functionalized materials on micro- and nano-scales (i.e. the labs) with optical fibers to realize miniaturized and advanced all-in-fiber probes, especially useful for (but not limited to) label-free chemical and biological applications. This review presents a broad overview of lab-on-fiber biosensors, with particular reference to lab-on-tip platforms, where the labs are integrated on the optical fiber facet. Light-matter interaction on the fiber tip is achieved through the integration of thin layers of nanoparticles or nanostructures supporting resonant modes, both plasmonic and photonic, highly sensitive to local modifications of the surrounding environment. According to the physical principle that is exploited, different configurations - such as localized plasmon resonance probes, surface enhanced Raman scattering probes and photonic probes - are classified, while various applications are presented in context throughout. For each device, the surface chemistry and the related functionalization protocols are reviewed. Moreover, the implementation strategies and fabrication processes, either based on bottom-up or top

A SYNCHRONIZED FIR/VUV LIGHT SOURCE AT JEFFERSON LAB

DOE Office of Scientific and Technical Information (OSTI.GOV)

Stephen Benson, David Douglas, George Neil, Michelle D. Shinn, Gwyn Williams

We describe a dual free-electron laser (FEL) configuration on the UV Demo FEL at Jefferson Lab that allows simultaneous lasing at FIR/THz and UV wavelengths. The FIR/THz source would be an FEL oscillator with a short wiggler providing nearly diffraction-limited pulses with pulse energy exceeding 50 microJoules. The FIR source would use the exhaust beam from a UVFEL. The coherent harmonics in the VUV from the UVFEL are out-coupled through a hole. The FIR source uses a shorter resonator with either hole or edge coupling to provide very high power FIR pulses. Simulations indicate excel-lent spectral brightness in the FIRmore » region with over 100 W/cm-1 output.« less

The NOAO Data Lab PHAT Photometry Database

NASA Astrophysics Data System (ADS)

Olsen, Knut; Williams, Ben; Fitzpatrick, Michael; PHAT Team

2018-01-01

We present a database containing both the combined photometric object catalog and the single epoch measurements from the Panchromatic Hubble Andromeda Treasury (PHAT). This database is hosted by the NOAO Data Lab (http://datalab.noao.edu), and as such exposes a number of data services to the PHAT photometry, including access through a Table Access Protocol (TAP) service, direct PostgreSQL queries, web-based and programmatic query interfaces, remote storage space for personal database tables and files, and a JupyterHub-based Notebook analysis environment, as well as image access through a Simple Image Access (SIA) service. We show how the Data Lab database and Jupyter Notebook environment allow for straightforward and efficient analyses of PHAT catalog data, including maps of object density, depth, and color, extraction of light curves of variable objects, and proper motion exploration.

Vortex Shedding Inside a Baffled Air Duct

NASA Technical Reports Server (NTRS)

Davis, Philip; Kenny, R. Jeremy

2010-01-01

Common in the operation of both segmented and un-segmented large solid rocket motors is the occurrence of vortex shedding within the motor chamber. A portion of the energy within a shed vortex is converted to acoustic energy, potentially driving the longitudinal acoustic modes of the motor in a quasi-discrete fashion. This vortex shedding-acoustic mode excitation event occurs for every Reusable Solid Rocket Motor (RSRM) operation, giving rise to subsequent axial thrust oscillations. In order to better understand this vortex shedding/acoustic mode excitation phenomena, unsteady CFD simulations were run for both a test geometry and the full scale RSRM geometry. This paper covers the results from the subscale geometry runs, which were based on work focusing on the RSRM hydrodynamics. Unsteady CFD simulation parameters, including boundary conditions and post-processing returns, are reviewed. The results were further post-processed to identify active acoustic modes and vortex shedding characteristics. Probable locations for acoustic energy generation, and subsequent acoustic mode excitation, are discussed.

The College of Charleston's 400-Student Observational Lab Program

NASA Astrophysics Data System (ADS)

True, C. M.

2006-06-01

For over thirty years the College of Charleston has been teaching a year-long introductory astronomy course incorporating a mandatory 3 hour lab. Despite our location in a very light polluted, coastal, high humidity, and often cloudy metropolitan area we have emphasized observational activities as much as possible. To accommodate our population of between 300-400 students per semester, we have 28 8-inch Celestron Telescopes and 25 GPS capable 8-inch Meade LX-200 telescopes. Finally, we have a 16 DFM adjacent to our rooftop observing decks. For indoor activities we have access to 42 computers running a variety of astronomy education software. Some of the computer activities are based on the Starry Night software (Backyard and Pro), the CLEA software from Gettysburg College, and Spectrum Explorer from Boston University. Additionally, we have labs involving cratering, eclipses and phases, coordinate systems with celestial globes, the inverse square law, spectroscopy and spectral classification, as well as others. In this presentation we will discuss the difficulties in managing a program of this size. We have approximately 14 lab sections a week. The lab manager's task involves coordinating 8-10 lab instructors and the same number of undergraduate teaching assistants as well as trying to maintain a coherent experience between the labs and lecture sections. Our lab manuals are produced locally with yearly updates. Samples from the manuals will be available. This program has been developed by a large number of College of Charleston astronomy faculty, including Don Drost, Bob Dukes, Chris Fragile, Tim Giblin, Jon Hakkila, Bill Kubinec, Lee Lindner, Jim Neff, Laura Penny, Al Rainis, Terry Richardson, and D. J. Williams, as well as adjunct and visiting faculty Bill Baird, Kevin Bourque, Ethan Denault, Kwayera Davis, Francie Halter, and Alan Johnson. Part of this work has been funded by NSF DUE grants to the College of Charleston.

e-Learning - Physics Labs

NASA Astrophysics Data System (ADS)

Mohottala, Hashini

2014-03-01

The general student population enrolled in any college level class is highly diverse. An increasing number of ``nontraditional'' students return to college and most of these students follow distance learning degree programs while engaging in their other commitments, work and family. However, those students tend to avoid taking science courses with labs, mostly because of the incapability of remotely completing the lab components in such courses. In order to address this issue, we have come across a method where introductory level physics labs can be taught remotely. In this process a lab kit with the critical lab components that can be easily accessible are conveniently packed into a box and distributed among students at the beginning of the semester. Once the students are given the apparatus they perform the experiments at home and gather data All communications with reference to the lab was done through an interactive user-friendly webpage - Wikispaces (WikiS). Students who create pages on WikiS can submit their lab write-ups, embed videos of the experiments they perform, post pictures and direct questions to the lab instructor. The students who are enrolled in the same lab can interact with each other through WikiS to discuss labs and even get assistance.

August 2007 FIFRA Scientific Advisory Panel Recommendations for SHEDS-Dietary and SHEDS-Residential Modules (Summarized) and EPA Responses

EPA Science Inventory

Over the past ten years, the Agency has requested the Panel to review several probabilistic dietary exposure software models. These have included DEEM-FCID™, Calendex-FCID, CARES™, LifeLine™, and an earlier (specialized) version of SHEDS (SHEDS-Wood) designed to assess exposure...

Microbes to Biomes at Berkeley Lab

DOE Office of Scientific and Technical Information (OSTI.GOV)

None

2015-10-28

Microbes are the Earth's most abundant and diverse form of life. Berkeley Lab's Microbes to Biomes initiative -- which will take advantage of research expertise at the Joint Genome Institute, Advanced Light Source, Molecular Foundry, and the new computational science facility -- is designed to explore and reveal the interactions of microbes with one another and with their environment. Microbes power our planet’s biogeochemical cycles, provide nutrients to our plants, purify our water and are integral components in keeping the human body free of disease and may hold the key to the Earth’s future.

19. WEST REAR AND SOUTH SIDE, SOUTH STORAGE SHED ATTACHED ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

19. WEST REAR AND SOUTH SIDE, SOUTH STORAGE SHED ATTACHED TO REAR OF HANGAR SHED. A SIMILAR NORTH STORAGE SHED IS VISIBLE IN THE DISTANCE. - Barstow-Daggett Airport, Hangar Shed No. 4, 39500 National Trails Highway, Daggett, San Bernardino County, CA

The "Green Lab": Power Consumption by Commercial Light Bulbs

ERIC Educational Resources Information Center

Einsporn, James A.; Zhou, Andrew F.

2011-01-01

Going "green" is a slogan that is very contemporary, both with industry and in the political arena. Choosing more energy-efficient devices is one way homeowners can "go green." A simple method is to change home lighting from hot incandescent bulbs to compact fluorescent lights (CFLs). But do they really save energy? How do their illuminations…

An LED solar simulator for student labs

NASA Astrophysics Data System (ADS)

González, Manuel I.

2017-05-01

Measuring voltage-current and voltage-power curves of a photovoltaic module is a nice experiment for high school and undergraduate students. In labs where real sunlight is not available this experiment requires a solar simulator. A prototype of a simulator using LED lamps has been manufactured and tested, and a comparison with classical halogen simulators has been performed. It is found that LED light offers lower levels of irradiance, but much better performance in terms of module output for a given irradiance.

A Comparative Study on Real Lab and Simulation Lab in Communication Engineering from Students' Perspectives

ERIC Educational Resources Information Center

Balakrishnan, B.; Woods, P. C.

2013-01-01

Over the years, rapid development in computer technology has engendered simulation-based laboratory (lab) in addition to the traditional hands-on (physical) lab. Many higher education institutions adopt simulation lab, replacing some existing physical lab experiments. The creation of new systems for conducting engineering lab activities has raised…

Kinematic Labs with Mobile Devices

NASA Astrophysics Data System (ADS)

Kinser, Jason M.

2015-07-01

This book provides 13 labs spanning the common topics in the first semester of university-level physics. Each lab is designed to use only the student's smartphone, laptop and items easily found in big-box stores or a hobby shop. Each lab contains theory, set-up instructions and basic analysis techniques. All of these labs can be performed outside of the traditional university lab setting and initial costs averaging less than 8 per student, per lab.

MethLAB

PubMed Central

Kilaru, Varun; Barfield, Richard T; Schroeder, James W; Smith, Alicia K

2012-01-01

Recent evidence suggests that DNA methylation changes may underlie numerous complex traits and diseases. The advent of commercial, array-based methods to interrogate DNA methylation has led to a profusion of epigenetic studies in the literature. Array-based methods, such as the popular Illumina GoldenGate and Infinium platforms, estimate the proportion of DNA methylated at single-base resolution for thousands of CpG sites across the genome. These arrays generate enormous amounts of data, but few software resources exist for efficient and flexible analysis of these data. We developed a software package called MethLAB (http://genetics.emory.edu/conneely/MethLAB) using R, an open source statistical language that can be edited to suit the needs of the user. MethLAB features a graphical user interface (GUI) with a menu-driven format designed to efficiently read in and manipulate array-based methylation data in a user-friendly manner. MethLAB tests for association between methylation and relevant phenotypes by fitting a separate linear model for each CpG site. These models can incorporate both continuous and categorical phenotypes and covariates, as well as fixed or random batch or chip effects. MethLAB accounts for multiple testing by controlling the false discovery rate (FDR) at a user-specified level. Standard output includes a spreadsheet-ready text file and an array of publication-quality figures. Considering the growing interest in and availability of DNA methylation data, there is a great need for user-friendly open source analytical tools. With MethLAB, we present a timely resource that will allow users with no programming experience to implement flexible and powerful analyses of DNA methylation data. PMID:22430798

Influence of Light Conditions and Light Sources on Clinical Measurement of Natural Teeth Color using VITA Easyshade Advance 4,0® Spectrophotometer. Pilot Study.

PubMed Central

Posavec, Ivona; Prpić, Vladimir

2016-01-01

Objectives The purpose of this study was to evaluate and compare lightness (L), chroma (C) and hue (h), green-red (a) and blue-yellow (b) character of the color of maxillary right central incisors in different light conditions and light sources. Materials and methods Two examiners who were well trained in digital color evaluation participated in the research. Intraclass correlation coefficients (ICCs) were used to analyze intra- and interobserver reliability. The LCh and L*a*b* values were determined at 08.15 and at 10.00 in the morning under three different light conditions. Tooth color was assessed in 10 subjects using intraoral spectrophotometer VITA Easyshade Advance 4.0® set at the central region of the vestibular surface of the measured tooth. Results Intra- and interobserver ICC values were high for both examiners and ranged from 0.57 to 0.99. Statistically significant differences in LCh and L*a*b* values measured in different time of the day and certain light condition were not found (p>0.05). Statistically significant differences in LCh and L*a*b* values measured under three different light conditions were not found, too (p>0.05). Conclusions VITA Easyshade Advance 4.0® is reliable enough for daily clinical work in order to assess tooth color during the fabrication of esthtic appliances because it is not dependent on light conditions and light sources. PMID:28275281

Influence of Light Conditions and Light Sources on Clinical Measurement of Natural Teeth Color using VITA Easyshade Advance 4,0® Spectrophotometer. Pilot Study.

PubMed

Posavec, Ivona; Prpić, Vladimir; Zlatarić, Dubravka Knezović

2016-12-01

The purpose of this study was to evaluate and compare lightness (L), chroma (C) and hue (h), green-red (a) and blue-yellow (b) character of the color of maxillary right central incisors in different light conditions and light sources. Two examiners who were well trained in digital color evaluation participated in the research. Intraclass correlation coefficients (ICCs) were used to analyze intra- and interobserver reliability. The LCh and L*a*b* values were determined at 08.15 and at 10.00 in the morning under three different light conditions. Tooth color was assessed in 10 subjects using intraoral spectrophotometer VITA Easyshade Advance 4.0 ® set at the central region of the vestibular surface of the measured tooth. Intra- and interobserver ICC values were high for both examiners and ranged from 0.57 to 0.99. Statistically significant differences in LCh and L*a*b* values measured in different time of the day and certain light condition were not found (p>0.05). Statistically significant differences in LCh and L*a*b* values measured under three different light conditions were not found, too (p>0.05). VITA Easyshade Advance 4.0 ® is reliable enough for daily clinical work in order to assess tooth color during the fabrication of esthtic appliances because it is not dependent on light conditions and light sources.

Teachers' Perspectives on Online Virtual Labs vs. Hands-On Labs in High School Science

NASA Astrophysics Data System (ADS)

Bohr, Teresa M.

This study of online science teachers' opinions addressed the use of virtual labs in online courses. A growing number of schools use virtual labs that must meet mandated laboratory standards to ensure they provide learning experiences comparable to hands-on labs, which are an integral part of science curricula. The purpose of this qualitative case study was to examine teachers' perceptions of the quality and effectiveness of high school virtual labs. The theoretical foundation was constructivism, as labs provide student-centered activities for problem solving, inquiry, and exploration of phenomena. The research questions focused on experienced teachers' perceptions of the quality of virtual vs. hands-on labs. Data were collected through survey questions derived from the lab objectives of The Next Generation Science Standards . Eighteen teachers rated the degree of importance of each objective and also rated how they felt virtual labs met these objectives; these ratings were reported using descriptive statistics. Responses to open-ended questions were few and served to illustrate the numerical results. Many teachers stated that virtual labs are valuable supplements but could not completely replace hands-on experiences. Studies on the quality and effectiveness of high school virtual labs are limited despite widespread use. Comprehensive studies will ensure that online students have equal access to quality labs. School districts need to define lab requirements, and colleges need to specify the lab experience they require. This study has potential to inspire positive social change by assisting science educators, including those in the local school district, in evaluating and selecting courseware designed to promote higher order thinking skills, real-world problem solving, and development of strong inquiry skills, thereby improving science instruction for all high school students.

HydroSHEDS: A global comprehensive hydrographic dataset

NASA Astrophysics Data System (ADS)

Wickel, B. A.; Lehner, B.; Sindorf, N.

2007-12-01

The Hydrological data and maps based on SHuttle Elevation Derivatives at multiple Scales (HydroSHEDS) is an innovative product that, for the first time, provides hydrographic information in a consistent and comprehensive format for regional and global-scale applications. HydroSHEDS offers a suite of geo-referenced data sets, including stream networks, watershed boundaries, drainage directions, and ancillary data layers such as flow accumulations, distances, and river topology information. The goal of developing HydroSHEDS was to generate key data layers to support regional and global watershed analyses, hydrological modeling, and freshwater conservation planning at a quality, resolution and extent that had previously been unachievable. Available resolutions range from 3 arc-second (approx. 90 meters at the equator) to 5 minute (approx. 10 km at the equator) with seamless near-global extent. HydroSHEDS is derived from elevation data of the Shuttle Radar Topography Mission (SRTM) at 3 arc-second resolution. The original SRTM data have been hydrologically conditioned using a sequence of automated procedures. Existing methods of data improvement and newly developed algorithms have been applied, including void filling, filtering, stream burning, and upscaling techniques. Manual corrections were made where necessary. Preliminary quality assessments indicate that the accuracy of HydroSHEDS significantly exceeds that of existing global watershed and river maps. HydroSHEDS was developed by the Conservation Science Program of the World Wildlife Fund (WWF) in partnership with the U.S. Geological Survey (USGS), the International Centre for Tropical Agriculture (CIAT), The Nature Conservancy (TNC), and the Center for Environmental Systems Research (CESR) of the University of Kassel, Germany.

Delineating resource sheds in aquatic ecosystems (presentation)

EPA Science Inventory

Analysis of spatially-explicit ecological phenomena in aquatic ecosystems is impeded by a lack of knowledge of, and tools to delimit, spatial patterns of material supply to point locations. Here we apply the concept of "resource sheds" to coasts and watersheds. Resource sheds ar...

TangoLab-2 Card Troubleshooting

NASA Image and Video Library

2017-10-17

iss053e105442 (Oct. 17, 2017) --- Flight Engineer Mark Vande Hei swaps out a payload card from the TangoLab-1 facility and places into the TangoLab-2 facility. TangoLab provides a standardized platform and open architecture for experimental modules called CubeLabs. CubeLab modules may be developed for use in 3-dimensional tissue and cell cultures.

A comparative study on real lab and simulation lab in communication engineering from students' perspectives

NASA Astrophysics Data System (ADS)

Balakrishnan, B.; Woods, P. C.

2013-05-01

Over the years, rapid development in computer technology has engendered simulation-based laboratory (lab) in addition to the traditional hands-on (physical) lab. Many higher education institutions adopt simulation lab, replacing some existing physical lab experiments. The creation of new systems for conducting engineering lab activities has raised concerns among educators on the merits and shortcomings of both physical and simulation labs; at the same time, many arguments have been raised on the differences of both labs. Investigating the effectiveness of both labs is complicated, as there are multiple factors that should be considered. In view of this challenge, a study on students' perspectives on their experience related to key aspects on engineering laboratory exercise was conducted. In this study, the Visual Auditory Read and Kinetic model was utilised to measure the students' cognitive styles. The investigation was done through a survey among participants from Multimedia University, Malaysia. The findings revealed that there are significant differences for most of the aspects in physical and simulation labs.

A Constructivist Cloud Lab.

ERIC Educational Resources Information Center

Emery, Dave

1996-01-01

Describes a lab involving a cloud formation activity that uses the constructivist learning model to get students more involved in creating the lab. Enables students to develop a greater understanding of the concepts involved and more interest in the lab's outcomes. (JRH)

Virtual Reality Lab Assistant

NASA Technical Reports Server (NTRS)

Saha, Hrishikesh; Palmer, Timothy A.

1996-01-01

Virtual Reality Lab Assistant (VRLA) demonstration model is aligned for engineering and material science experiments to be performed by undergraduate and graduate students in the course as a pre-lab simulation experience. This will help students to get a preview of how to use the lab equipment and run experiments without using the lab hardware/software equipment. The quality of the time available for laboratory experiments can be significantly improved through the use of virtual reality technology.

LabSkills

ERIC Educational Resources Information Center

O'Brien, Nick

2010-01-01

This article describes LabSkills, a revolutionary teaching tool to improve practical science in schools. LabSkills offers the chance to help improve the exposure that the average Key Stage 5 (age 16-19) student has to practical work. This is a huge area for development being highlighted by universities who are seeing a worryingly growing trend in…

Microbes to Biomes at Berkeley Lab

ScienceCinema

None

2018-06-21

Microbes are the Earth's most abundant and diverse form of life. Berkeley Lab's Microbes to Biomes initiative -- which will take advantage of research expertise at the Joint Genome Institute, Advanced Light Source, Molecular Foundry, and the new computational science facility -- is designed to explore and reveal the interactions of microbes with one another and with their environment. Microbes power our planet’s biogeochemical cycles, provide nutrients to our plants, purify our water and are integral components in keeping the human body free of disease and may hold the key to the Earth’s future.

2. SOUTH FACE OF PYROTECHNIC SHED (BLDG. 757) SHOWING SIGN ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

2. SOUTH FACE OF PYROTECHNIC SHED (BLDG. 757) SHOWING SIGN HOLDER ON LEFT AND ENTRANCE TO TEST CELL. METEOROLOGICAL TOWER AND METEOROLOGICAL SHED (BLDG. 756) IN BACKGROUND ON LEFT; SOUTHEAST CORNER OF GPS AZIMUTH STATION (BLDG. 775) IN BACKGROUND BEHIND AND RIGHT OF PYROTECHNIC SHED. - Vandenberg Air Force Base, Space Launch Complex 3, Pyrotechnic Shed, Napa & Alden Roads, Lompoc, Santa Barbara County, CA

The International Globe at Night Citizen-Science Campaign: Shedding Light on Light Pollution

NASA Astrophysics Data System (ADS)

Walker, C. E.; Pompea, S. M.

2013-12-01

For 8 years now, the Globe at Night campaign has invited citizen-scientists worldwide to measure and record the brightness of their night sky by hunting for the faintest stars in a particular constellation. Students for science projects and scientists for research use the data to monitor levels of brightness or 'light pollution' around the world. They also use the Globe at Night data to understand light pollution's effects on energy consumption, plants, wildlife and human health, as well as our ability to enjoy a starry night sky. The dates of the campaign for 2014 have been extended to every month during the year. Ten days each month (when the Moon is not up between 8pm and 10pm) are the recommended times to take measurements for the campaign. However, one can participate at other times and dates, as long as the Moon is not in the night sky and it is more than an hour after sunset or more than an hour before sunrise. New in 2014 will be an Android app that will allow you to input visual measurements anytime the Moon is not up. Also possibly included will be an iPhone app that will take sky brightness measurements. The campaign dates and the 5 easy steps to participating in the campaign are listed at www.globeatnight.org. You do not need to register. Once on the report page, you enter your location, date and time (automatic for a smart device). You find the constellation of the month in the night sky. (Help is on the website.) Then you choose which chart looks most like what you see toward the constellation. Choose the icon for how clear or cloudy it is and hit the submit button and you are done! The fifth step is returning later to the website to compare your observations on the world map to others from around the globe. Included on the Globe at Night website are many helpful resources and tools from finding the constellations used in the campaign, to understanding concepts like light pollution, to games that test your expertise in choosing 'limiting magnitudes

Counter and Complicit Masculine Discourse Among Men's Shed Members.

PubMed

Mackenzie, Corey S; Roger, Kerstin; Robertson, Steve; Oliffe, John L; Nurmi, Mary Anne; Urquhart, James

2017-07-01

Men's Sheds is a growing international movement aimed at providing men with places and activities that facilitate social connectedness. Despite Men's Sheds' focus on males, little attention has been paid to masculinities within the specific context of these settings. The current study used a gender relations framework to explore the ways in which attendees discussed Men's Sheds, with particular attention to discussions that were complicit and counter to traditional, hegemonic views of masculinity, and diverse positions in between these binaries. The data consisted of transcripts and field notes from four focus groups comprising mostly older, White, retired male members of a Canadian shed ( N = 22). The analysis revealed three overall themes: (1) focus on work, (2) independence, and (3) need for male-focused spaces. These themes and associated subthemes suggest that shed members ascribe to dominant masculine values and ideals, but also support more fluid and flexible views of masculinity. Implications are discussed for how working with an array of masculinities within the Men's Sheds movement will be helpful with respect to their future growth in Canada and internationally.

Numerical Simulations of Vortex Shedding in Hydraulic Turbines

NASA Technical Reports Server (NTRS)

Dorney, Daniel; Marcu, Bogdan

2004-01-01

Turbomachines for rocket propulsion applications operate with many different working fluids and flow conditions. Oxidizer boost turbines often operate in liquid oxygen, resulting in an incompressible flow field. Vortex shedding from airfoils in this flow environment can have adverse effects on both turbine performance and durability. In this study the effects of vortex shedding in a low-pressure oxidizer turbine are investigated. Benchmark results are also presented for vortex shedding behind a circular cylinder. The predicted results are compared with available experimental data.

Solid State Light Evaluation in the U.S. Lab Mockup

NASA Technical Reports Server (NTRS)

Maida, James c.; Bowen, Charles K.; Wheelwright, Chuck

2009-01-01

This document constitutes the publication of work performed by the Space Human Factors Laboratory (mail code SF5 at the time) at the Johnson Space Center (JSC) in the months of June and July of 2000. At that time, the Space Human Factors Laboratory was part of the Space Human Factors Branch in the Flight Projects Division of the Space and Life Directorate. This report was originally to be a document for internal consumption only at JSC as it was seen to be only preliminary work for the further development of solid state illumination for general lighting on future space vehicles and the International Space Station (ISS). Due to funding constraints, immediate follow-on efforts were delayed and the need for publication of this document was overcome by other events. However, in recent years and with the development and deployment of a solid state light luminaire prototype on ISS, the time was overdue for publishing this information for general distribution and reference. Solid state lights (SSLs) are being developed to potentially replace the general luminaire assemblies (GLAs) currently in service in the International Space Station (ISS) and included in designs of modules for the ISS. The SSLs consist of arrays of light emitting diodes (LEDs), small solid state electronic devices that produce visible light in proportion to the electrical current flowing through them. Recent progressive advances in electrical power-to-light conversion efficiency in LED technology have allowed the consideration of LEDs as replacements for incandescent and fluorescent light sources in many circumstances, and their inherent advantages in ruggedness, reliability, and life expectancy make them attractive for applications in spacecraft. One potential area of application for the SSLs in the U.S. Laboratory Module of the ISS. This study addresses the suitability of the SSLs as replacements for the GLAs in this application.

A Proteomic Approach to Characterize Protein Shedding

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ahram, Mamoun; Adkins, Joshua N.; Auberry, Deanna L.

2005-01-01

Shedding (i.e., proteolysis of ectodomains of membrane proteins) plays an important pathophysiological role. In order to study the feasibility of identifying shed proteins, we analyzed serum-free media of human mammary epithelial cells by mass spectrometry following induction of shedding by the phorbol ester, 4β-phorbol 12-myristate 13-acetate (PMA). Different means of sample preparation, including biotinylation of cell surface proteins, isolation of glycosylated proteins, and preparation of crude protein fraction, were carried out to develop the optimal method of sample processing. The collected proteins were digested with trypsin and analyzed by reversed-phase capillary liquid chromatography interfaced to an ion-trap mass spectrometer. Themore » resulting peptide spectra were interpreted using the program SEQUEST. Analyzing the sample containing the crude protein mixture without chemical modification or separation resulted in the greatest number of identifications, including putatively shed proteins. Overall, 93 membrane-associated proteins were identified including 57 that contain at least one transmembrane domain and 36 that indirectly associate with the extracellular surface of the plasma membrane. Of the 57 transmembrane proteins, 43 were identified by extracellular peptides providing strong evidence for them originating from regulated proteolysis or shedding processes. We combined results from the different experiments and used a peptide count method to estimate changes in protein abundance. Using this approach, we identified 2 proteins, syndecan-4 and hepatoma-derived growth factor, whose abundances increased in media of cells treated with PMA. We also detected proteins whose abundances decreased after PMA treatment such as 78-kDa glucose-regulated protein and calreticulin. Further analysis using immunoblotting validated the abundance changes for syndecan-4 and 78-kDa glucose-regulated protein as a result of PMA treatment. These results demonstrate that

Men's Sheds and the experience of depression in older Australian men.

PubMed

Culph, Jennifer S; Wilson, Nathan J; Cordier, Reinie; Stancliffe, Roger J

2015-10-01

Men's Sheds are community spaces where, usually, older men can socialise as they participate in a range of woodwork and other activities. There is currently little research evidence supporting the anecdotally reported mental health and wellbeing benefits of Men's Sheds. This research project investigated how older men with self-reported symptoms of depression experience their participation in Men's Sheds. This study included in-depth interviews and administration of the Beck Depression Inventory-II with 12 men from 3 Men's Sheds, triangulated with observation of the different shed environments. Interviews explored how participation in the Men's Shed, living in a regional area, and retirement intersected with experiences of depression. Participants had either self-reported symptoms of depression or a diagnosis of depression. The findings from this study support the notion that participation at Men's Sheds decreases self-reported symptoms of depression. Beck Depression Inventory-II scores showed that most participants were currently experiencing minimal depression. The Men's Sheds environment promoted a sense of purpose through relationships and in the sharing of skills, new routines, motivation, and enjoyment for its members. The shed encouraged increased physical activity and use of cognitive skills. Finally, participants reported feelings of pride and achievement which had an impact on their sense of self-worth. Men's Sheds provide an opportunity to promote health and wellbeing among retired men. The shed's activity and social focus offers a way to help men rediscover purpose and self. Further research is required to measure symptoms of depression before and after participation in Men's Sheds. © 2015 Occupational Therapy Australia.

Usage of DNA Fingerprinting Technology for Quality Control in Molecular Lab Bench Work.

PubMed

McIntosh, Linda Y; Lal, Janella E; Qin, Dahui

2018-01-01

One of the major quality assurance (QA) goals in many molecular laboratories is to avoid sample pipetting errors on the lab bench; especially when pipetting into multiwell plates. A pipetting error can cause a switch in patient samples, which can lead to recording the wrong results for the patient samples involved. Such pipetting errors are difficult to identify when it happens in lab bench work. DNA fingerprinting is a powerful tool in determining sample identities. Our laboratory has explored the usage of this technology in our QA process and successfully established that DNA fingerprinting can be used to monitor possible sample switch in gene rearrangement lab bench work. We use florescent light to quench the florescence in the gene rearrangement polymerase chain reaction products. After that, DNA fingerprinting technology is used to identify the sample DNA in the gene rearrangement polymerase chain reaction plate. The result is compared with the corresponding patient's blood sample DNA to determine whether there is a sample switch during the lab bench work.

Sustainable dual-use labs: neurovascular interventional capabilities within the cath lab.

PubMed

Lang, Stacey

2012-01-01

The inclusion of neurovascular interventional capabilities within the cath lab setting can be key to optimal utilization of resources, increased staff efficiency, and streamlined operations. When considering an expansion, look beyond the patient population traditionally associated with cardiac cath labs and consider the integration of programs outside cardiac alone--to create a true dual-use lab space. With proper planning, quality dual purpose equipment, appropriately trained staff, capable physicians, and strong leadership, an organization willing to embrace the challenge can build a truly extraordinary service.

Lab at Home: Hardware Kits for a Digital Design Lab

ERIC Educational Resources Information Center

Oliver, J. P.; Haim, F.

2009-01-01

An innovative laboratory methodology for an introductory digital design course is presented. Instead of having traditional lab experiences, where students have to come to school classrooms, a "lab at home" concept is proposed. Students perform real experiments in their own homes, using hardware kits specially developed for this purpose. They…

Shedding Light on the Battlefield: Tactical Applications of Photonic Technology

DTIC Science & Technology

2004-11-01

of the first applications of photonics to communications was the photophone ,1 demonstrated by Alexander Graham Bell in 1880, which used light beams to...transmit information wirelessly. Bell believed the invention of the photophone was more significant than that of the telephone, but it took almost... photophone used sunlight and vibrating mirrors to transmit the human voice. http://inventors.about.com/library/inventors/bltelephone3.htm, accessed April

Separation of scintillation and Cherenkov lights in linear alkyl benzene

DOE PAGES

Li, Mohan; Guo, Ziyi; Yeh, Minfang; ...

2016-09-11

To separate scintillation and Cherenkov lights in water-based liquid scintillator detectors is a desired feature for future neutrino and proton decay experiments. Linear alkyl benzene (LAB) is one important ingredient of a water-based liquid scintillator currently under development. In this paper we report on the separation of scintillation and Cherenkov lights observed in an LAB sample. The rise and decay times of the scintillation light are measured to be (7.7±3.0)ns and (36.6±2.4)ns, respectively, while the full width [–3σ, 3σ] of the Cherenkov light is 12 ns and is dominated by the time resolution of the photomultiplier tubes. Here, the scintillationmore » light yield was measured to be (1.01±0.12)×103photons/MeV.« less

Jet substructure shedding light on heavy Majorana neutrinos at the LHC

NASA Astrophysics Data System (ADS)

Das, Arindam; Konar, Partha; Thalapillil, Arun

2018-02-01

The existence of tiny neutrino masses and flavor mixings can be explained naturally in various seesaw models, many of which typically having additional Majorana type SM gauge singlet right handed neutrinos ( N). If they are at around the electroweak scale and furnished with sizable mixings with light active neutrinos, they can be produced at high energy colliders, such as the Large Hadron Collider (LHC). A characteristic signature would be same sign lepton pairs, violating lepton number, together with light jets — pp → Nℓ ± , N → ℓ ± W ∓ , W ∓ → jj. We propose a new search strategy utilising jet substructure techniques, observing that for a heavy right handed neutrino mass M N much above M W ±, the two jets coming out of the boosted W ± may be interpreted as a single fat-jet ( J). Hence, the distinguishing signal topology will be ℓ ± ℓ ± J . Performing a comprehensive study of the different signal regions along with complete background analysis, in tandem with detector level simulations, we compute statistical significance limits. We find that heavy neutrinos can be explored effectively for mass ranges 300 GeV ≤ M N ≤ 800 GeV and different light-heavy neutrino mixing | V μN |2. At the 13 TeV LHC with 3000 fb-1 integrated luminosity one can competently explore mixing angles much below present LHC limits, and moreover exceed bounds from electroweak precision data.

LCOGT Imaging Lab

NASA Astrophysics Data System (ADS)

Tufts, Joseph R.; Lobdill, Rich; Haldeman, Benjamin J.; Haynes, Rachel; Hawkins, Eric; Burleson, Ben; Jahng, David

2008-07-01

The Las Cumbres Observatory Global Telescope Network (LCOGT) is an ambitious project to build and operate, within 5 years, a worldwide robotic network of 50 0.4, 1, and 2 m telescopes sharing identical instrumentation and optimized for precision photometry of time-varying sources. The telescopes, instrumentation, and software are all developed in house with two 2 m telescopes already installed. The LCOGT Imaging Lab is responsible for assembly and characterization of the network's cameras and instrumentation. In addition to a fully equipped CNC machine shop, two electronics labs, and a future optics lab, the Imaging Lab is designed from the ground up to be a superb environment for bare detectors, precision filters, and assembled instruments. At the heart of the lab is an ISO class 5 cleanroom with full ionization. Surrounding this, the class 7 main lab houses equipment for detector characterization including QE and CTE, and equipment for measuring transmission and reflection of optics. Although the first science cameras installed, two TEC cooled e2v 42-40 deep depletion based units and two CryoTiger cooled Fairchild Imaging CCD486-BI based units, are from outside manufacturers, their 18 position filter wheels and the remainder of the network's science cameras, controllers, and instrumentation will be built in house. Currently being designed, the first generation LCOGT cameras for the network's 1 m telescopes use existing CCD486-BI devices and an in-house controller. Additionally, the controller uses digital signal processing to optimize readout noise vs. speed, and all instrumentation uses embedded microprocessors for communication over ethernet.

Inexpensive DAQ based physics labs

NASA Astrophysics Data System (ADS)

Lewis, Benjamin; Clark, Shane

2015-11-01

Quality Data Acquisition (DAQ) based physics labs can be designed using microcontrollers and very low cost sensors with minimal lab equipment. A prototype device with several sensors and documentation for a number of DAQ-based labs is showcased. The device connects to a computer through Bluetooth and uses a simple interface to control the DAQ and display real time graphs, storing the data in .txt and .xls formats. A full device including a larger number of sensors combined with software interface and detailed documentation would provide a high quality physics lab education for minimal cost, for instance in high schools lacking lab equipment or students taking online classes. An entire semester’s lab course could be conducted using a single device with a manufacturing cost of under $20.

Nanoscale interfacial defect shedding in a growing nematic droplet.

PubMed

Gurevich, Sebastian; Provatas, Nikolas; Rey, Alejandro

2017-08-01

Interfacial defect shedding is the most recent known mechanism for defect formation in a thermally driven isotropic-to-nematic phase transition. It manifests in nematic-isotropic interfaces going through an anchoring switch. Numerical computations in planar geometry established that a growing nematic droplet can undergo interfacial defect shedding, nucleating interfacial defect structures that shed into the bulk as +1/2 point defects. By extending the study of interfacial defect shedding in a growing nematic droplet to larger length and time scales, and to three dimensions, we unveil an oscillatory growth mode involving shape and anchoring transitions that results in a controllable regular distributions of point defects in planar geometry, and complex structures of disclination lines in three dimensions.

MatLab Script and Functional Programming

NASA Technical Reports Server (NTRS)

Shaykhian, Gholam Ali

2007-01-01

MatLab Script and Functional Programming: MatLab is one of the most widely used very high level programming languages for scientific and engineering computations. It is very user-friendly and needs practically no formal programming knowledge. Presented here are MatLab programming aspects and not just the MatLab commands for scientists and engineers who do not have formal programming training and also have no significant time to spare for learning programming to solve their real world problems. Specifically provided are programs for visualization. The MatLab seminar covers the functional and script programming aspect of MatLab language. Specific expectations are: a) Recognize MatLab commands, script and function. b) Create, and run a MatLab function. c) Read, recognize, and describe MatLab syntax. d) Recognize decisions, loops and matrix operators. e) Evaluate scope among multiple files, and multiple functions within a file. f) Declare, define and use scalar variables, vectors and matrices.

Seeing the Light (LBNL Science at the Theater)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Brunger, Axel; Segalman, Rachel; Westphal, Andrew

2011-09-12

Berkeley Lab's Science at the Theater event "Seeing the Light" took place on Sept 12, 2011, at Berkeley Repertory's Roda Theatre. Learn how the Advanced Light Source is improving medicine, paving the way for clean energy, changing the future of computers, and much more. Featured speakers are Berkeley Lab's Roger Falcone, Rachel Segalman, Andrew Westphal, and Stanford University's Axel Brunger. Rachel Segalman: The future of clean energy technology relies on a better understanding of materials at the nanoscale. Berkeley Lab's Rachel Segalman uses the ALS to conduct this research, which could lead to improved photovoltaics and fuel cells. Axel Brunger:more » Improved treatment for human diseases hinges on understanding molecular-scale processes. Stanford University's Axel Brunger will discuss a new melanoma drug that was developed by a local company, Plexxikon, using the ALS for X-ray data collection. Andrew Westphal: What's comet dust made of? Andrew Westphal of UC Berkeley's Space Sciences Laboratory uses the ALS to study comet dust and interplanetary space dust collected by a NASA spacecraft. Moderated by Roger Falcone, Division Director of the Advanced Light Source« less

PatternLab for proteomics 4.0: A one-stop shop for analyzing shotgun proteomic data

PubMed Central

Carvalho, Paulo C; Lima, Diogo B; Leprevost, Felipe V; Santos, Marlon D M; Fischer, Juliana S G; Aquino, Priscila F; Moresco, James J; Yates, John R; Barbosa, Valmir C

2017-01-01

PatternLab for proteomics is an integrated computational environment that unifies several previously published modules for analyzing shotgun proteomic data. PatternLab contains modules for formatting sequence databases, performing peptide spectrum matching, statistically filtering and organizing shotgun proteomic data, extracting quantitative information from label-free and chemically labeled data, performing statistics for differential proteomics, displaying results in a variety of graphical formats, performing similarity-driven studies with de novo sequencing data, analyzing time-course experiments, and helping with the understanding of the biological significance of data in the light of the Gene Ontology. Here we describe PatternLab for proteomics 4.0, which closely knits together all of these modules in a self-contained environment, covering the principal aspects of proteomic data analysis as a freely available and easily installable software package. All updates to PatternLab, as well as all new features added to it, have been tested over the years on millions of mass spectra. PMID:26658470

Experiences with lab-centric instruction

NASA Astrophysics Data System (ADS)

Titterton, Nathaniel; Lewis, Colleen M.; Clancy, Michael J.

2010-06-01

Lab-centric instruction emphasizes supervised, hands-on activities by substituting lab for lecture time. It combines a multitude of pedagogical techniques into the format of an extended, structured closed lab. We discuss the range of benefits for students, including increased staff interaction, frequent and varied self-assessments, integrated collaborative activities, and a systematic sequence of activities that gradually increases in difficulty. Instructors also benefit from a deeper window into student progress and understanding. We follow with discussion of our experiences in courses at U.C. Berkeley, and using data from some of these investigate the effects of lab-centric instruction on student learning, procrastination, and course pacing. We observe that the lab-centric format helped students on exams but hurt them on extended programming assignments, counter to our hypothesis. Additionally, we see no difference in self-ratings of procrastination and limited differences in ratings of course pace. We do find evidence that the students who choose to attend lab-centric courses are different in several important ways from students who choose to attend the same course in a non-lab-centric format.

Credit PSR. The flammable waste materials shed appears as seen ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Credit PSR. The flammable waste materials shed appears as seen when looking south (186°) from South Liquid Loop Road. Note the catch basin for retaining accidentally spilled substances. Wastes are stored in drums and other safety containers until disposal by burning at the Incinerator (4249/E-50) or by other means. Note the nearby sign warning of corrosive, flammable materials, and calling attention to a fire extinguisher; a telephone is provided to call for assistance in the event of an emergency. This structure is isolated to prevent the spread of fire, and it is lightly built so damage from a fire will be inexpensive to repair - Jet Propulsion Laboratory Edwards Facility, Waste Flammable Storage Building, Edwards Air Force Base, Boron, Kern County, CA

EarthLabs: A National Model for Earth Science Lab Courses

NASA Astrophysics Data System (ADS)

McDaris, J. R.; Dahlman, L.; Barstow, D.

2008-12-01

As a response to the need for more rigorous, inquiry-based high school Earth science courses, a coalition of scientists, educators, and five states have created EarthLabs, a set of pilot modules that can serve as a national model for lab-based science courses. The content of EarthLabs chapters focuses on Earth system science and environmental literacy and conforms to the National Science Education Standards as well as the states' curriculum frameworks. The effort is funded by NOAA's Environmental Literacy program. The pilot modules present activities on Corals, Drought, Fisheries, and Hurricanes. The Fisheries and Hurricanes units were reviewed and field-tested by educators in Texas and Arizona. The feedback from this evaluation led to revisions of these units and guided development of the Corals and Drought chapters. Each module consists of activities that use online data sets, satellite imagery, web-based readings, and hands-on laboratory experiments. The project comprises two separate websites, one for the instructor and one for students. The instructor's site contains the pedagogical underpinnings for each lab including teaching materials, assessment strategies, and the alignment of activities with state and national science standards. The student site provides access to all materials that students need to complete the activities or, in the case of the hands-on labs, where they access additional information to help extend their learning. There are also formative and summative questions embedded in the student webpages to help scaffold learning through the activities.

Improving the Quality of Lab Reports by Using Them as Lab Instructions

NASA Astrophysics Data System (ADS)

Haagen-Schuetzenhoefer, Claudia

2012-10-01

Lab exercises are quite popular in teaching science. Teachers have numerous goals in mind when teaching science laboratories. Nevertheless, empirical research draws a heterogeneous picture of the benefits of lab work. Research has shown that it does not necessarily contribute to the enhancement of practical abilities or content knowledge. Lab activities are frequently based on recipe-like, step-by-step instructions ("cookbook style"), which do not motivate students to engage cognitively. Consequently, students put the emphasis on "task completion" or "manipulating equipment."2

Reviews Book: The 4% Universe: Dark Matter, Dark Energy and the Race to Discover the Rest of Reality Book: Quantitative Understanding of Biosystems: An Introduction to Biophysics Book: Edison's Electric Light: The Art of Invention Book: The Edge of Physics: Dispatches from the Frontiers of Cosmology Equipment: Voicebox Equipment: Tracker 4 Books: Hands-On Introduction to NI LabVIEW with Vernier, and Engineering Projects with NI LabVIEW and Vernier Places to Visit: Discovery Museum Book: Philosophy of Science: A Very Short Introduction Web Watch

NASA Astrophysics Data System (ADS)

2011-11-01

WE RECOMMEND Quantitative Understanding of Biosystems: An Introduction to Biophysics Text applies physics to biology concepts Edison's Electric Light: The Art of Invention Edison's light still shines brightly The Edge of Physics: Dispatches from the Frontiers of Cosmology Anecdotes explore cosmology Voicebox Voicebox kit discovers the physics and evolution of speech Tracker 4 Free software tracks motion analysis Hands-On Introduction to NI LabVIEW with Vernier, and Engineering Projects with NI LabVIEW and Vernier Books support the LabVIEW software Discovery Museum Newcastle museum offers science enjoyment for all Philosophy of Science: A Very Short Introduction Philosophy opens up science questions WORTH A LOOK The 4% Universe: Dark Matter, Dark Energy and the Race to Discover the Rest of Reality Book researches the universe WEB WATCH Superconductivity websites are popular

German physicians "on strike"--shedding light on the roots of physician dissatisfaction.

PubMed

Janus, Katharina; Amelung, Volker Eric; Gaitanides, Michael; Schwartz, Friedrich Wilhelm

2007-08-01

, continuous education and job security, administrative tasks and collegial relationships were highly significant, specialized technology and patient contact were significant and research and teaching and international exchange were not significant in contributing to physician job satisfaction. This study sheds light to the underlying factors that contribute to physician job satisfaction in Germany, and it provides insights into the reasons for physicians leaving medical practice. In order for a health system to recruit and retain physicians, it may be necessary for a system's physician strategy to shift from focusing primarily on hard, monetary and compensation-related factors to a broader focus that incorporates the soft, non-monetary factors. The implementation of policies and management practices that reduce the time burden on physicians, and enhance physicians' participation in the development of patient care management processes and in managerial decisions that affect patient care appears to be crucial. The result will be increased job satisfaction among physicians, which is important to the future recruitment and retention of doctors, as well as to the productivity and quality of the services provided by this essential component of our medical care systems.

Vortex shedding in bileaflet heart valve prostheses.

PubMed

Gross, J M; Shermer, C D; Hwang, N H

1988-01-01

A dynamic study of two geometrically similar bileaflet heart valve prostheses (HVP) was performed using a physiologic mock circulatory flow loop. The HVPs studied were the 25 mm St. Jude Medical (SJM) and the 25 mm Carbomedics (CMI) in the aortic position and the 27 mm SJM and 27 mm CMI in the mitral position. All data were collected at a heart rate of 70 beats/min and a cardiac output of 5.0 L/min. Flow visualization was conducted in the transparent flow chambers of the pulsatile mock circulatory flow loop using a 15 mW He-Ne laser light source. A cylindrical lens and optics system converted the incident laser beam into a thin parallel light plane, and 420 microns tracer particles were suspended in the testing fluid to illuminate the flow field at selected planes. Frame-by-frame analysis of the 16 mm high-speed cine provides detailed phasic flow patterns in the vicinity of the HVP. A series of still photographs of flow patterns, taken at approximately 22.5 degrees phase intervals, are sequentially presented for each HVP. In the aortic position, a Karman-like vortex pattern appears downstream of the SJM at the end of the ejection phase. The CMI exhibits a rather symmetrical ejection flow pattern that turns into random motion immediately after the onset of ejection. In the mitral position, the SJM again exhibits a strong core flow during ventricular filling, whereas the CMI produces a more diffuse pattern during the same period. A pair of vortices shed from both the SJM and CMI are clearly visible toward the end of the ventricular filling phase. The vortex mechanisms are discussed in light of leaflet boundary layer formation.

Lab Report Blues

ERIC Educational Resources Information Center

Diaz, Andrew

2004-01-01

For middle school students, writing a formal lab report can be challenging. For middle level teachers, reading students lab reports can be overwhelming. After grading report after report with incomplete procedures, incorrect graphs, and missing conclusions, the author's frustration level was at an all-time high. Ready to try anything, he thought,…

Reforming Cookbook Labs

ERIC Educational Resources Information Center

Peters, Erin

2005-01-01

Deconstructing cookbook labs to require the students to be more thoughtful could break down perceived teacher barriers to inquiry learning. Simple steps that remove or disrupt the direct transfer of step-by-step procedures in cookbook labs make students think more critically about their process. Through trials in the author's middle school…

1. PAINT AND OIL STORAGE SHED, FRONT, LOOKING SOUTHWEST. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

1. PAINT AND OIL STORAGE SHED, FRONT, LOOKING SOUTHWEST. - NIKE Missile Base SL-40, Paint & Oil Storage Shed, North end of base, northwest of Mess Hall & south of Basketball Court, Hecker, Monroe County, IL

Systemic Immune Activation and HIV Shedding in the Female Genital Tract.

PubMed

Spencer, LaShonda Y; Christiansen, Shawna; Wang, Chia-Hao H; Mack, Wendy J; Young, Mary; Strickler, Howard D; Anastos, Kathryn; Minkoff, Howard; Cohen, Mardge; Geenblatt, Ruth M; Karim, Roksana; Operskalski, Eva; Frederick, Toni; Homans, James D; Landay, Alan; Kovacs, Andrea

2016-02-01

Plasma HIV RNA is the most significant determinant of cervical HIV shedding. However, shedding is also associated with sexually transmitted infections (STIs) and cervical inflammation. The mechanism by which this occurs is poorly understood. There is evidence that systemic immune activation promotes viral entry, replication, and HIV disease progression. We hypothesized that systemic immune activation would be associated with an increase in HIV genital shedding. Clinical assessments, HIV RNA in plasma and genital secretions, and markers of immune activation (CD38(+)DR(+) and CD38(-)DR(-)) on CD4(+) and CD8(+) T cells in blood were evaluated in 226 HIV+ women enrolled in the Women's Interagency HIV Study. There were 569 genital evaluations of which 159 (28%) exhibited HIV RNA shedding, defined as HIV viral load >80 copies per milliliter. We tested associations between immune activation and shedding using generalized estimating equations with logit link function. In the univariate model, higher levels of CD4(+) and CD8(+) T-cell activation in blood were significantly associated with genital tract shedding. However, in the multivariate model adjusting for plasma HIV RNA, STIs, and genital tract infections, only higher levels of resting CD8(+) T cells (CD38(-)DR(-)) were significantly inversely associated with HIV shedding in the genital tract (odds ratios = 0.44, 95% confidence interval: 0.21 to 0.9, P = 0.02). The association of systemic immune activation with genital HIV shedding is multifactorial. Systemic T-cell activation is associated with genital tract shedding in univariate analysis but not when adjusting for plasma HIV RNA, STIs, and genital tract infections. In addition, women with high percentage of resting T cells are less likely to have HIV shedding compared with those with lower percentages. These findings suggest that a higher percentage of resting cells, as a result of maximal viral suppression with treatment, may decrease local genital activation, HIV

Status of chemistry lab safety in Nepal.

PubMed

Kandel, Krishna Prasad; Neupane, Bhanu Bhakta; Giri, Basant

2017-01-01

Chemistry labs can become a dangerous environment for students as the lab exercises involve hazardous chemicals, glassware, and equipment. Approximately one hundred thousand students take chemistry laboratory classes annually in Nepal. We conducted a survey on chemical lab safety issues across Nepal. In this paper, we assess the safety policy and equipment, protocols and procedures followed, and waste disposal in chemistry teaching labs. Significant population of the respondents believed that there is no monitoring of the lab safety in their lab (p<0.001). Even though many labs do not allow food and beverages inside lab and have first aid kits, they lack some basic safety equipment. There is no institutional mechanism to dispose lab waste and chemical waste is disposed haphazardly. Majority of the respondents believed that the safety training should be a part of educational training (p = 0.001) and they would benefit from short course and/or workshop on lab safety (p<0.001).

Status of chemistry lab safety in Nepal

PubMed Central

Kandel, Krishna Prasad; Neupane, Bhanu Bhakta

2017-01-01

Chemistry labs can become a dangerous environment for students as the lab exercises involve hazardous chemicals, glassware, and equipment. Approximately one hundred thousand students take chemistry laboratory classes annually in Nepal. We conducted a survey on chemical lab safety issues across Nepal. In this paper, we assess the safety policy and equipment, protocols and procedures followed, and waste disposal in chemistry teaching labs. Significant population of the respondents believed that there is no monitoring of the lab safety in their lab (p<0.001). Even though many labs do not allow food and beverages inside lab and have first aid kits, they lack some basic safety equipment. There is no institutional mechanism to dispose lab waste and chemical waste is disposed haphazardly. Majority of the respondents believed that the safety training should be a part of educational training (p = 0.001) and they would benefit from short course and/or workshop on lab safety (p<0.001). PMID:28644869

Houses and Sheds in Australia: An Exploration of the Genesis and Growth of Neighbourhood Houses and Men's Sheds in Community Settings

ERIC Educational Resources Information Center

Golding, Barry; Kimberley, Helen; Foley, Annette; Brown, Mike

2008-01-01

This article reviews research into the genesis and spread of both neighbourhood houses and learning centres in Victoria and community-based men's sheds in Australia to identify some similarities and differences. Our article asks questions about the gendered communities of practice that underpin houses for women on the one hand, and sheds for men…

Analysis and Prediction of Ice Shedding for a Full-Scale Heated Tail Rotor

NASA Technical Reports Server (NTRS)

Kreeger, Richard E.; Work, Andrew; Douglass, Rebekah; Gazella, Matthew; Koster, Zakery; Turk, Jodi

2016-01-01

When helicopters are to fly in icing conditions, it is necessary to consider the possibility of ice shed from the rotor blades. In 2013, a series of tests were conducted on a heated tail rotor at NASA Glenn's Icing Research Tunnel (IRT). The tests produced several shed events that were captured on camera. Three of these shed events were captured at a sufficiently high frame rate to obtain multiple images of the shed ice in flight that had a sufficiently long section of shed ice for analysis. Analysis of these shed events is presented and compared to an analytical Shedding Trajectory Model (STM). The STM is developed and assumes that the ice breaks off instantly as it reaches the end of the blade, while frictional and viscous forces are used as parameters to fit the STM. The trajectory of each shed is compared to that predicted by the STM, where the STM provides information of the shed group of ice as a whole. The limitations of the model's underlying assumptions are discussed in comparison to experimental shed events.

Light-Induced Capacitance Tunability in Ferroelectric Crystals.

PubMed

Páez-Margarit, David; Rubio-Marcos, Fernando; Ochoa, Diego A; Del Campo, Adolfo; Fernández, José F; García, José E

2018-06-25

The remote controlling of ferroic properties with light is nowadays a hot and highly appealing topic in materials science. Here, we shed light on some of the unresolved issues surrounding light-matter coupling in ferroelectrics. Our findings show that the capacitance and, consequently, its related intrinsic material property, i.e., the dielectric constant, can be reversibly adjusted through the light power control. High photodielectric performance is exhibited across a wide range of the visible light wavelength because of the wavelength-independence of the phenomenon. We have verified that this counterintuitive behavior can be strongly ascribed to the existence of "locally free charges" at domain wall.

A comparison of CIE L*a*b* and spectral methods for the analysis of fading in sliced cured ham

NASA Astrophysics Data System (ADS)

Sheridan, C.; O'Farrell, M.; Lewis, E.; Flanagan, C.; Kerry, J.; Jackman, N.

2007-06-01

In the modern retail environment, the appearance of a product is frequently the only quality indicator available to consumers. This is especially true of products such as sliced ham that have been sealed into packages to maintain product freshness. It has been shown that sliced ham products undergo discolouration from their original pink colour to a pale grey colour when exposed to a combination of oxygen and light. This is unappealing to consumers who expect a pink colour for sliced ham. An investigation is made into a sensor that would monitor the initial colour status of cured ham before packaging in order to determine the amount of time left before the ham fades to an unsatisfactory colour. For this sensor to operate, appropriate analysis of the appearance of the meat is required. Two methods for the measurement of the fading were investigated—CIE L*a*b* measurements and analysis of the spectral reflectance of the colour of the ham. Several sliced ham products with differing amounts of fading were examined using both methods. It was observed that the products used covered a wide range of variation in colour. Reproducibility of CIE L*a*b* values proved to be quite difficult and significant overlapping of the L* (lightness) and a* (redness) values measured for pink and grey coloured ham was observed. The variations in these values can be attributed to differences in the intensity of reflected light for different products. L*a*b* measurements are sensitive to light intensity and pigment concentration. Analysis of the spectral reflectance readings did not encounter these problems as the spectral response was normalized (to reduce intensity errors) before data analysis was carried out on the spectral shape or 'pattern' using principal component analysis (PCA) and artificial neural networks (ANN). A classifier based on PCA and ANN was successfully implemented that can discriminate different stages of fading for the ham slices. A case study was carried out on ham

SenseLab

PubMed Central

Crasto, Chiquito J.; Marenco, Luis N.; Liu, Nian; Morse, Thomas M.; Cheung, Kei-Hoi; Lai, Peter C.; Bahl, Gautam; Masiar, Peter; Lam, Hugo Y.K.; Lim, Ernest; Chen, Huajin; Nadkarni, Prakash; Migliore, Michele; Miller, Perry L.; Shepherd, Gordon M.

2009-01-01

This article presents the latest developments in neuroscience information dissemination through the SenseLab suite of databases: NeuronDB, CellPropDB, ORDB, OdorDB, OdorMapDB, ModelDB and BrainPharm. These databases include information related to: (i) neuronal membrane properties and neuronal models, and (ii) genetics, genomics, proteomics and imaging studies of the olfactory system. We describe here: the new features for each database, the evolution of SenseLab’s unifying database architecture and instances of SenseLab database interoperation with other neuroscience online resources. PMID:17510162

Shedding light on neutrino masses with dark forces

DOE Office of Scientific and Technical Information (OSTI.GOV)

Batell, Brian; Pospelov, Maxim; Shuve, Brian

Heavy right-handed neutrinos, N , provide the simplest explanation for the origin of light neutrino masses and mixings. If M N is at or below the weak scale, direct experimental discovery of these states is possible at accelerator experiments such as the LHC or new dedicated beam dump experiments; in these experiments, N decays after traversing a macroscopic distance from the collision point. The experimental sensitivity to right-handed neutrinos is significantly enhanced if there is a new “dark” gauge force connecting them to the Standard Model (SM), and detection of N can be the primary discovery mode for the newmore » dark force itself. We take the well-motivated example of a B – L gauge symmetry and analyze the sensitivity to displaced decays of N produced via the new gauge interaction in two experiments: the LHC and the proposed SHiP beam dump experiment. In the most favorable case in which the mediator can be produced on-shell and decays to right handed neutrinos (pp → X + V B–L → X + N N ), the sensitivity reach is controlled by the square of the B – L gauge coupling. Here, we demonstrate that these experiments could access neutrino parameters responsible for the observed SM neutrino masses and mixings in the most straightforward implementation of the see-saw mechanism.« less

Shedding light on neutrino masses with dark forces

DOE PAGES

Batell, Brian; Pospelov, Maxim; Shuve, Brian

2016-08-08

Heavy right-handed neutrinos, N , provide the simplest explanation for the origin of light neutrino masses and mixings. If M N is at or below the weak scale, direct experimental discovery of these states is possible at accelerator experiments such as the LHC or new dedicated beam dump experiments; in these experiments, N decays after traversing a macroscopic distance from the collision point. The experimental sensitivity to right-handed neutrinos is significantly enhanced if there is a new “dark” gauge force connecting them to the Standard Model (SM), and detection of N can be the primary discovery mode for the newmore » dark force itself. We take the well-motivated example of a B – L gauge symmetry and analyze the sensitivity to displaced decays of N produced via the new gauge interaction in two experiments: the LHC and the proposed SHiP beam dump experiment. In the most favorable case in which the mediator can be produced on-shell and decays to right handed neutrinos (pp → X + V B–L → X + N N ), the sensitivity reach is controlled by the square of the B – L gauge coupling. Here, we demonstrate that these experiments could access neutrino parameters responsible for the observed SM neutrino masses and mixings in the most straightforward implementation of the see-saw mechanism.« less

The personal and community impact of a Scottish Men's Shed.

PubMed

Foster, Emma J; Munoz, Sarah-Anne; Leslie, Stephen J

2018-02-21

Social isolation and loneliness are known to be associated with increased morbidity and mortality. Therefore, reducing social isolation and loneliness may improve such outcomes. In relation to men's health, "Men's Sheds" have been shown as one mechanism to achieve this. Studies in Australia and England have shown social, health and personal benefits; however, this remains an area that has not yet been researched in Scotland. This study, therefore, aimed to assess the characteristics of attendees, self-reported motivations for and the values and benefits of attending the Shed from the views of the attendees themselves. The participants of the study were the members of a Men's Shed in the North of Scotland, which was initially set-up by a small number of core Shedders. A convenience sample was recruited by opportunistic interviewing of participants when they attended the Shed using a mixed methods approach from 1 to 15 November 2016. In the absence of a validated questionnaire, a bespoke questionnaire was developed in several iterative stages. The answers to the questionnaire were transferred to an electronic database and analysed by frequency and thematic analysis. The participants (n = 31) had a mean age (SD) of 69.7 ± 9.5 with 96.8% being retired, thus the majority of the Shed users were older and retired. The results suggest that there were several benefits from attending the Shed, with an overwhelming majority of the sample reporting personal, social and health benefits-however, more research is needed to determine the magnitude of these. This study has also shown that the men attending the Shed frequently discussed health, which could potentially have a beneficial effect. The Shed therefore, as a community project, has the potential to have a positive impact on health welfare by focusing on the social aspects of life. © 2018 John Wiley & Sons Ltd.

Alternative mode of presentation of Kanizsa figures sheds new light on the chronometry of the mechanisms underlying the perception of illusory figures.

PubMed

Brodeur, M; Lepore, F; Lepage, M; Bacon, B A; Jemel, B; Debruille, J B

2008-01-31

The mechanisms responsible for the perception of illusory modal figures are usually studied by presenting entire Kanizsa figures at stimulus onset. However, with this mode of presentation, the brain activity generated by the inducers (the 'pacmen') is difficult to differentiate from the activity underlying the perception of the illusory figure. Therefore, in addition to this usual presentation mode, we used an alternative presentation mode. Inducer disks remained permanently on the screen and the illusory figure was induced by just removing the notches from the disks. The results support the heuristic value of this alternative mode of presentation. The P1 deflection of the visual evoked potentials (VEPs) was found to be greater for the illusory modal figure than for its control and for an amodal figure. This modulation is one of the earliest direct evidences for a low-level processing of illusory forms in the human brain. Meanwhile, larger N1s were obtained for the control figures than for the illusory figures in the notch mode of presentation. While this new type of N1 modulation could shed some light on the stage of processing indexed by this deflection, several propositions are put forward to account for the P1 and N1 variations found.

Integrating Robotic Observatories into Astronomy Labs

NASA Astrophysics Data System (ADS)

Ruch, Gerald T.

2015-01-01

The University of St. Thomas (UST) and a consortium of five local schools is using the UST Robotic Observatory, housing a 17' telescope, to develop labs and image processing tools that allow easy integration of observational labs into existing introductory astronomy curriculum. Our lab design removes the burden of equipment ownership by sharing access to a common resource and removes the burden of data processing by automating processing tasks that are not relevant to the learning objectives.Each laboratory exercise takes place over two lab periods. During period one, students design and submit observation requests via the lab website. Between periods, the telescope automatically acquires the data and our image processing pipeline produces data ready for student analysis. During period two, the students retrieve their data from the website and perform the analysis. The first lab, 'Weighing Jupiter,' was successfully implemented at UST and several of our partner schools. We are currently developing a second lab to measure the age of and distance to a globular cluster.

The Role of Collaborative Learning on Training and Development Practices within the Australian Men's Shed Movement: A Study of Five Men's Sheds

ERIC Educational Resources Information Center

Cavanagh, Jillian; Southcombe, Amie; Bartram, Tim

2014-01-01

This study examines the role and impact of collaborative learning on training and development practices in Australian Men's Sheds. We use a case study approach, underpinned by Peters and Armstrong's theoretical framework of collaborative learning in adult education, to investigate five Men's Sheds. Semi-structured interviews were carried out with…

Replication and shedding kinetics of infectious hematopoietic necrosis virus in juvenile rainbow trout

USGS Publications Warehouse

Wargo, Andrew R.; Scott, Robert J.; Kerr, Benjamin; Kurath, Gael

2017-01-01

Viral replication and shedding are key components of transmission and fitness, the kinetics of which are heavily dependent on virus, host, and environmental factors. To date, no studies have quantified the shedding kinetics of infectious hematopoietic necrosis virus (IHNV) in rainbow trout (Oncorhynchus mykiss), or how they are associated with replication, making it difficult to ascertain the transmission dynamics of this pathogen of high agricultural and conservation importance. Here, the replication and shedding kinetics of two M genogroup IHNV genotypes were examined in their naturally co-evolved rainbow trout host. Within host virus replication began rapidly, approaching maximum values by day 3 post-infection, after which viral load was maintained or gradually dropped through day 7. Host innate immune response measured as stimulation of Mx-1 gene expression generally followed within host viral loads. Shedding also began very quickly and peaked within 2 days, defining a generally uniform early peak period of shedding from 1 to 4 days after exposure to virus. This was followed by a post-peak period where shedding declined, such that the majority of fish were no longer shedding by day 12 post-infection. Despite similar kinetics, the average shedding rate over the course of infection was significantly lower in mixed compared to single genotype infections, suggesting a competition effect, however, this did not significantly impact the total amount of virus shed. The data also indicated that the duration of shedding, rather than peak amount of virus shed, was correlated with fish mortality. Generally, the majority of virus produced during infection appeared to be shed into the environment rather than maintained in the host, although there was more retention of within host virus during the post-peak period. Viral virulence was correlated with shedding, such that the more virulent of the two genotypes shed more total virus. This fundamental understanding of IHNV

Replication and shedding kinetics of infectious hematopoietic necrosis virus in juvenile rainbow trout.

PubMed

Wargo, Andrew R; Scott, Robert J; Kerr, Benjamin; Kurath, Gael

2017-01-02

Viral replication and shedding are key components of transmission and fitness, the kinetics of which are heavily dependent on virus, host, and environmental factors. To date, no studies have quantified the shedding kinetics of infectious hematopoietic necrosis virus (IHNV) in rainbow trout (Oncorhynchus mykiss), or how they are associated with replication, making it difficult to ascertain the transmission dynamics of this pathogen of high agricultural and conservation importance. Here, the replication and shedding kinetics of two M genogroup IHNV genotypes were examined in their naturally co-evolved rainbow trout host. Within host virus replication began rapidly, approaching maximum values by day 3 post-infection, after which viral load was maintained or gradually dropped through day 7. Host innate immune response measured as stimulation of Mx-1 gene expression generally followed within host viral loads. Shedding also began very quickly and peaked within 2days, defining a generally uniform early peak period of shedding from 1 to 4days after exposure to virus. This was followed by a post-peak period where shedding declined, such that the majority of fish were no longer shedding by day 12 post-infection. Despite similar kinetics, the average shedding rate over the course of infection was significantly lower in mixed compared to single genotype infections, suggesting a competition effect, however, this did not significantly impact the total amount of virus shed. The data also indicated that the duration of shedding, rather than peak amount of virus shed, was correlated with fish mortality. Generally, the majority of virus produced during infection appeared to be shed into the environment rather than maintained in the host, although there was more retention of within host virus during the post-peak period. Viral virulence was correlated with shedding, such that the more virulent of the two genotypes shed more total virus. This fundamental understanding of IHNV shedding

Replication and shedding kinetics of infectious hematopoietic necrosis virus in juvenile rainbow trout

PubMed Central

Wargo, Andrew R.; Scott, Robert J.; Kerr, Benjamin; Kurath, Gael

2016-01-01

Viral replication and shedding are key components of transmission and fitness, the kinetics of which are heavily dependent on virus, host, and environmental factors. To date, no studies have quantified the shedding kinetics of infectious hematopoietic necrosis virus (IHNV) in rainbow trout (Oncorhynchus mykiss), or how they are associated with replication, making it difficult to ascertain the transmission dynamics of this pathogen of high agricultural and conservation importance. Here, the replication and shedding kinetics of two M genogroup IHNV genotypes were examined in their naturally co-evolved rainbow trout host. Within host virus replication began rapidly, approaching maximum values by day 3 post-infection, after which viral load was maintained or gradually dropped through day 7. Host innate immune response measured as stimulation of Mx-1 gene expression generally followed within host viral loads. Shedding also began very quickly and peaked within 2 days, defining a generally uniform early peak period of shedding from 1 to 4 days after exposure to virus. This was followed by a post-peak period where shedding declined, such that the majority offish were no longer shedding by day 12 post-infection. Despite similar kinetics, the average shedding rate over the course of infection was significantly lower in mixed compared to single genotype infections, suggesting a competition effect, however, this did not significantly impact the total amount of virus shed. The data also indicated that the duration of shedding, rather than peak amount of virus shed, was correlated with fish mortality. Generally, the majority of virus produced during infection appeared to be shed into the environment rather than maintained in the host, although there was more retention of within host virus during the post-peak period. Viral virulence was correlated with shedding, such that the more virulent of the two genotypes shed more total virus. This fundamental understanding of IHNV

SHEDDING LIGHT ON CORALS HEALTH: INTERACTIONS OF CLIMATE CHANGE AND SOLAR RADIATION WITH BLEACHING

EPA Science Inventory

Coral bleaching and declines in coral reef health in recent years have been attributed in part to processes driven by UV and/or visible light. For coral assemblages, exposure to UV light is often an unavoidable consequence of having access to visible (photosynthetically active) ...

Shedding Light on Bird Egg Color: Pigment as Parasol and the Dark Car Effect.

PubMed

Lahti, David C; Ardia, Daniel R

2016-05-01

The vibrant colors of many birds' eggs, particularly those that are blue to blue-green, are extraordinary in that they are striking traits present in hundreds of species that have nevertheless eluded evolutionary functional explanation. We propose that egg pigmentation mediates a trade-off between two routes by which solar radiation can harm bird embryos: transmittance through the eggshell and overheating through absorbance. We quantitatively test four components of this hypothesis on variably colored eggs of the village weaverbird (Ploceus cucullatus) in a controlled light environment: (1) damaging ultraviolet radiation can transmit through bird eggshells, (2) infrared radiation at natural intensities can heat the interior of eggs, (3) more intense egg coloration decreases light transmittance ("pigment as parasol"), and (4) more intense egg coloration increases absorbance of light by the eggshell and heats the egg interior ("dark car effect"). Results support all of these predictions. Thus, in sunlit nesting environments, less pigmentation will increase the detrimental effect of transmittance, but more pigmentation will increase the detrimental effect of absorbance. The optimal pigmentation level for a bird egg in a given light environment, all other things being equal, will depend on the balance between light transmittance and absorbance in relation to embryo fitness.

Light-Duty Vehicle Thermal Management | Transportation Research | NREL

Science.gov Websites

Light-Duty Vehicle Thermal Management Light-Duty Vehicle Thermal Management Image of a semi transportation options, the lab is working to optimize the thermal management of both electric-drive and fuel per year just to air-condition these LDVs. NREL evaluates the effectiveness of thermal management

Improving the Quality of Lab Reports by Using Them as Lab Instructions

ERIC Educational Resources Information Center

Haagen-Schuetzenhoefer, Claudia

2012-01-01

Lab exercises are quite popular in teaching science. Teachers have numerous goals in mind when teaching science laboratories. Nevertheless, empirical research draws a heterogeneous picture of the benefits of lab work. Research has shown that it does not necessarily contribute to the enhancement of practical abilities or content knowledge. Lab…

Experimental investigation on cavitating flow shedding over an axisymmetric blunt body

NASA Astrophysics Data System (ADS)

Hu, Changli; Wang, Guoyu; Huang, Biao

2015-03-01

Nowadays, most researchers focus on the cavity shedding mechanisms of unsteady cavitating flows over different objects, such as 2D/3D hydrofoils, venturi-type section, axisymmetric bodies with different headforms, and so on. But few of them pay attention to the differences of cavity shedding modality under different cavitation numbers in unsteady cavitating flows over the same object. In the present study, two kinds of shedding patterns are investigated experimentally. A high speed camera system is used to observe the cavitating flows over an axisymmetric blunt body and the velocity fields are measured by a particle image velocimetry (PIV) technique in a water tunnel for different cavitation conditions. The U-type cavitating vortex shedding is observed in unsteady cavitating flows. When the cavitation number is 0.7, there is a large scale cavity rolling up and shedding, which cause the instability and dramatic fluctuation of the flows, while at cavitation number of 0.6, the detached cavities can be conjunct with the attached part to induce the break-off behavior again at the tail of the attached cavity, as a result, the final shedding is in the form of small scale cavity and keeps a relatively steady flow field. It is also found that the interaction between the re-entrant flow and the attached cavity plays an important role in the unsteady cavity shedding modality. When the attached cavity scale is insufficient to overcome the re-entrant flow, it deserves the large cavity rolling up and shedding just as that at cavitation number of 0.7. Otherwise, the re-entrant flow is defeated by large enough cavity to induce the cavity-combined process and small scale cavity vortexes shedding just as that of the cavitation number of 0.6. This research shows the details of two different cavity shedding modalities which is worthful and meaningful for the further study of unsteady cavitation.

Computational Labs Using VPython Complement Conventional Labs in Online and Regular Physics Classes

NASA Astrophysics Data System (ADS)

Bachlechner, Martina E.

2009-03-01

Fairmont State University has developed online physics classes for the high-school teaching certificate based on the text book Matter and Interaction by Chabay and Sherwood. This lead to using computational VPython labs also in the traditional class room setting to complement conventional labs. The computational modeling process has proven to provide an excellent basis for the subsequent conventional lab and allows for a concrete experience of the difference between behavior according to a model and realistic behavior. Observations in the regular class room setting feed back into the development of the online classes.

Incorporation of a PbSe Array Based Spectrograph into EPICS using LabView at the JLab FEL Facility

DOE Office of Scientific and Technical Information (OSTI.GOV)

D. Hardy; S.V. Benson; Michelle D. Shinn

2005-08-21

A real-time spectrograph with a 1Hz update rate was designed and installed at the JLab FEL facility using a Cal Sensors PbSe array and a Roper Scientific SpectraPro 300 monochrometer. This paper describes the implementation of EPICS channel access on a remote PC running LabView with modification of vendor supplied LabView VI's to allow display of FEL light spectra in real-time on a remote workstation. This allows PC based diagnostics to be used in EPICS.

West elevation of shed. Metal siding covers up the original ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

West elevation of shed. Metal siding covers up the original front of what was once the Middlekauf shoe shop. - Kreider-Reisner Aircraft Company, Shed, 851 Pennsylvania Avenue, Hagerstown, Washington County, MD

Spaceport Processing System Development Lab

NASA Technical Reports Server (NTRS)

Dorsey, Michael

2013-01-01

The Spaceport Processing System Development Lab (SPSDL), developed and maintained by the Systems Hardware and Engineering Branch (NE-C4), is a development lab with its own private/restricted networks. A private/restricted network is a network with restricted or no communication with other networks. This allows users from different groups to work on their own projects in their own configured environment without interfering with others utilizing their resources in the lab. The different networks being used in the lab have no way to talk with each other due to the way they are configured, so how a user configures his software, operating system, or the equipment doesn't interfere or carry over on any of the other networks in the lab. The SPSDL is available for any project in KSC that is in need of a lab environment. My job in the SPSDL was to assist in maintaining the lab to make sure it's accessible for users. This includes, but is not limited to, making sure the computers in the lab are properly running and patched with updated hardware/software. In addition to this, I also was to assist users who had issues in utilizing the resources in the lab, which may include helping to configure a restricted network for their own environment. All of this was to ensure workers were able to use the SPSDL to work on their projects without difficulty which would in turn, benefit the work done throughout KSC. When I wasn't working in the SPSDL, I would instead help other coworkers with smaller tasks which included, but wasn't limited to, the proper disposal, moving of, or search for essential equipment. I also, during the free time I had, used NASA's resources to increase my knowledge and skills in a variety of subjects related to my major as a computer engineer, particularly in UNIX, Networking, and Embedded Systems.

Subclinical herpesvirus shedding among HIV-1-infected men on antiretroviral therapy.

PubMed

Agudelo-Hernandez, Arcadio; Chen, Yue; Bullotta, Arlene; Buchanan, William G; Klamar-Blain, Cynthia R; Borowski, Luann; Riddler, Sharon A; Rinaldo, Charles R; Macatangay, Bernard J C

2017-09-24

We evaluated the subclinical shedding of six different herpesviruses in antiretroviral drug-treated HIV-positive [HIV(+)] MSM, and determined how this is associated with markers of inflammation and immune activation. We obtained blood, semen, throat washing, urine, and stool from 15 antiretroviral-treated HIV-1-infected MSM with CD4 T-cell reconstitution, and 12 age-matched HIV-negative [HIV (-)] MSM from the Multicenter AIDS Cohort Study at four timepoints over 24 weeks to measure DNA levels of cytomegalovirus (CMV), Epstein-Barr virus (EBV), herpes simplex virus 1 and 2, human herpesvirus 6 (HHV6), and HHV8. T-cell activation and plasma levels of soluble markers of inflammation and activation were also measured at the corresponding timepoints. HIV(+) participants had a trend for higher total herpesvirus shedding rate. HIV(+) participants also had a significantly higher rate of shedding EBV and CMV compared with the HIV(-) group. Herpesvirus shedding was mostly seen in throat washings. In the HIV(+) group, herpesvirus shedding rate inversely correlated with plasma levels of interferon γ-induced protein 10 and soluble CD163. CMV DNA levels negatively correlated with levels of T-cell activation. There was a trend for a positive correlation between EBV shedding rate and plasma soluble CD14. HHV6 shedding rate negatively correlated with plasma levels of interleukin-6, soluble CD163, and interferon gamma-induced protein 10. Correlations were not observed among HIV(-) individuals. Among treated HIV-infected MSM, there are higher subclinical shedding rates of some herpesviruses that occur in different body compartments and negatively correlate with levels of inflammation and immune activation.

4. MACHINERY SHED AND STORAGE ROOM ADDITION, SOUTH AND WEST ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

4. MACHINERY SHED AND STORAGE ROOM ADDITION, SOUTH AND WEST WALL LOOKING NORTHEAST SEED STORAGE BUILDING (1963) BEHIND - Tucson Plant Material Center, Machinery Shed, 3241 North Romero Road, Tucson, Pima County, AZ

Capacity building in indigenous men's groups and sheds across Australia.

PubMed

Southcombe, Amie; Cavanagh, Jillian; Bartram, Timothy

2015-09-01

This article presents an investigation into capacity building, at the community level, in Aboriginal and Torres Strait Islander Men's Groups and Sheds. As safe men's spaces, Men's Groups and Sheds represent an ever-growing social, and health and well-being community service across Australia. The study is qualitative and employs 'yarning circles' (focus groups), semi-structured interviews and observations to gather data from 15 Groups/Sheds involving 45 men from urban, regional and remote communities. We found that capacity building is primarily about securing relationships between Group Leaders/Shed Co-ordinators and Government services. Capacity building establishes links to services such as Centrelink, Medicare, Department of Housing, Probation and Control, and positive outcomes such as Indigenous men securing housing and Centrelink payments. Capacity building results in better health outcomes and, educates and empowers men to improve their social, cultural, emotional and economic well-being. It helps men to better connect with family and community. The current research paves the way for countries worldwide to explore the conceptual and empirical approach of capacity building applicable to other Indigenous [and non-Indigenous] Men's Groups/Sheds. We recommend feasibilities studies, on approaches to capacity building in Indigenous Groups/Sheds, be carried out within urban, regional and remote regions across the country. © The Author (2014). Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

14. GENERAL OBLIQUE VIEW OF WEST CORNER OF SHED, OBSTRUCTED ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

14. GENERAL OBLIQUE VIEW OF WEST CORNER OF SHED, OBSTRUCTED BY LATE METAL BUILDING, LOOKING EAST - Oakland Army Base, Transit Shed, East of Dunkirk Street & South of Burma Road, Oakland, Alameda County, CA

3. OBLIQUE GENERAL VIEW SHOWING EAST CORNER OF SHED, WITH ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

3. OBLIQUE GENERAL VIEW SHOWING EAST CORNER OF SHED, WITH RAILROAD TRACKS PASSING UNDER DERRICK ALONG WHARF - Oakland Army Base, Transit Shed, East of Dunkirk Street & South of Burma Road, Oakland, Alameda County, CA

2. ACID STORAGE SHED, FRONT AND RIGHT SIDES, LOOKING SOUTHWEST. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

2. ACID STORAGE SHED, FRONT AND RIGHT SIDES, LOOKING SOUTHWEST. - NIKE Missile Base C-84, Acid Storage Shed, North of launch area, northwest of earthen berm of Acid Fueling Station, Barrington, Cook County, IL

East elevation of bunkhouse and agriculture storage sheds, looking west. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

East elevation of bunkhouse and agriculture storage sheds, looking west. The shower house is adjacent to the storage sheds and just south of the bunkhouse. - Sespe Ranch, Bunkhouse, 2896 Telegraph Road, Fillmore, Ventura County, CA

2. SOUTH FACE OF METEOROLOGICAL SHED (BLDG. 756) WITH METEOROLOGICAL ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

2. SOUTH FACE OF METEOROLOGICAL SHED (BLDG. 756) WITH METEOROLOGICAL DATA ACQUISITION TERMINAL (MDAT) INSIDE BUILDING - Vandenberg Air Force Base, Space Launch Complex 3, Meteorological Shed & Tower, Napa & Alden Roads, Lompoc, Santa Barbara County, CA

2. SHED, SOUTH END OF SHORTER BARRACKS, FRONT AND RIGHT ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

2. SHED, SOUTH END OF SHORTER BARRACKS, FRONT AND RIGHT SIDES, LOOKING SOUTHWEST. - NIKE Missile Base C-84, Paint & Oil Storage Shed, South of Launch Area Entrance Drive, near security fence, Barrington, Cook County, IL

View of EPA Farm storage shed, facing north. Greenhouse is ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

View of EPA Farm storage shed, facing north. Greenhouse is in background - Nevada Test Site, Environmental Protection Agency Farm, Storage Shed, Area 15, Yucca Flat, 10-2 Road near Circle Road, Mercury, Nye County, NV

Cherry picker at end of Train Shed with arm fully ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Cherry picker at end of Train Shed with arm fully extended and photographer in bucket - Central of Georgia Railway, Passenger Station & Train Shed, Corner of Louisville (Railroad) Road & West Broad Street, Savannah, Chatham County, GA

SHEDS-HT: An Integrated Probabilistic Exposure Model for ...

EPA Pesticide Factsheets

United States Environmental Protection Agency (USEPA) researchers are developing a strategy for highthroughput (HT) exposure-based prioritization of chemicals under the ExpoCast program. These novel modeling approaches for evaluating chemicals based on their potential for biologically relevant human exposures will inform toxicity testing and prioritization for chemical risk assessment. Based on probabilistic methods and algorithms developed for The Stochastic Human Exposure and Dose Simulation Model for Multimedia, Multipathway Chemicals (SHEDS-MM), a new mechanistic modeling approach has been developed to accommodate high-throughput (HT) assessment of exposure potential. In this SHEDS-HT model, the residential and dietary modules of SHEDS-MM have been operationally modified to reduce the user burden, input data demands, and run times of the higher-tier model, while maintaining critical features and inputs that influence exposure. The model has been implemented in R; the modeling framework links chemicals to consumer product categories or food groups (and thus exposure scenarios) to predict HT exposures and intake doses. Initially, SHEDS-HT has been applied to 2507 organic chemicals associated with consumer products and agricultural pesticides. These evaluations employ data from recent USEPA efforts to characterize usage (prevalence, frequency, and magnitude), chemical composition, and exposure scenarios for a wide range of consumer products. In modeling indirec

9. GENERAL OBLIQUE VIEW OF SOUTH CORNER OF SHED WITH ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

9. GENERAL OBLIQUE VIEW OF SOUTH CORNER OF SHED WITH DERRICK AND RAILWAY PASS-TROUGH ON WHARF, LOOKING NORTH - Oakland Army Base, Transit Shed, East of Dunkirk Street & South of Burma Road, Oakland, Alameda County, CA

1. GENERAL VIEW SHOWING NORTHEAST END (FRONT) OF TRANSIT SHED, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

1. GENERAL VIEW SHOWING NORTHEAST END (FRONT) OF TRANSIT SHED, IN CONTEXT WITH LOADING YARD AND DERRICK, LOOKING WEST - Oakland Army Base, Transit Shed, East of Dunkirk Street & South of Burma Road, Oakland, Alameda County, CA

7. GENERAL VIEW OF SOUTHEAST SIDE OF SHED, SHOWING ALL ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

7. GENERAL VIEW OF SOUTHEAST SIDE OF SHED, SHOWING ALL EIGHTEEN LOADING BAYS, LOOKING WEST FROM ACROSS TURNING BASIN - Oakland Army Base, Transit Shed, East of Dunkirk Street & South of Burma Road, Oakland, Alameda County, CA

Salmonella Fecal Shedding and Immune Responses are Dose- and Serotype- Dependent in Pigs

PubMed Central

Ivanek, Renata; Österberg, Julia; Gautam, Raju; Sternberg Lewerin, Susanna

2012-01-01

Despite the public health importance of Salmonella infection in pigs, little is known about the associated dynamics of fecal shedding and immunity. In this study, we investigated the transitions of pigs through the states of Salmonella fecal shedding and immune response post-Salmonella inoculation as affected by the challenge dose and serotype. Continuous-time multistate Markov models were developed using published experimental data. The model for shedding had four transient states, of which two were shedding (continuous and intermittent shedding) and two non-shedding (latency and intermittent non-shedding), and one absorbing state representing permanent cessation of shedding. The immune response model had two transient states representing responses below and above the seroconversion level. The effects of two doses [low (0.65×106 CFU/pig) and high (0.65×109 CFU/pig)] and four serotypes (Salmonella Yoruba, Salmonella Cubana, Salmonella Typhimurium, and Salmonella Derby) on the models' transition intensities were evaluated using a proportional intensities model. Results indicated statistically significant effects of the challenge dose and serotype on the dynamics of shedding and immune response. The time spent in the specific states was also estimated. Continuous shedding was on average 10–26 days longer, while intermittent non-shedding was 2–4 days shorter, in pigs challenged with the high compared to low dose. Interestingly, among pigs challenged with the high dose, the continuous and intermittent shedding states were on average up to 10–17 and 3–4 days longer, respectively, in pigs infected with S. Cubana compared to the other three serotypes. Pigs challenged with the high dose of S. Typhimurium or S. Derby seroconverted on average up to 8–11 days faster compared to the low dose. These findings highlight that Salmonella fecal shedding and immune response following Salmonella challenge are dose- and serotype-dependent and that the detection of specific

2. PAINT AND OIL STORAGE SHED, FRONT AND RIGHT SIDES, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

2. PAINT AND OIL STORAGE SHED, FRONT AND RIGHT SIDES, LOOKING SOUTH. - NIKE Missile Base SL-40, Paint & Oil Storage Shed, North end of base, northwest of Mess Hall & south of Basketball Court, Hecker, Monroe County, IL

Depression, Anxiety, and Stress Symptoms in Menopausal Arab Women: Shedding More Light on a Complex Relationship.

PubMed

Bener, A; Saleh, N M; Bakir, A; Bhugra, D

2016-01-01

The association between depression, anxiety, and stress among Arab menopause and postmenopausal women have been explored in detailed. The objective of this study was to determine the correlation between depression, anxiety, and stress in menopausal and postmenopausal women and shedding more light on a complex relationship. A cross-sectional descriptive study was used to generate menopause symptoms experienced by Arabian women at the primary health care centers in Qatar. A representative sample of 1468 women aged 45-65 years were approached during July 2012 and May 2014 and 1101 women agreed to participate (75.0%) and responded to the study. Depression, anxiety, and stress were measured using the Depression Anxiety Stress Scales 21. Data on body mass index (BMI), clinical and other parameters were used. Univariate, multivariate, and matrix correlation analysis were performed for statistical analysis. A total of 1101 women agreed to participate after informed consent was obtained. The mean age and standard deviation (SD) of the menopausal age were 49.55 (3.12), the mean and SD of postmenopausal age was 58.08 (3.26) ( P < 0.001). There were statistically significant differences between menopausal stages with regards to age, ethnicity, educational status, occupation status, and place of living. Furthermore, there were statistically significant differences between menopausal stages with regards to BMI, systolic and diastolic blood pressure (BP), Vitamin D deficiency, and diseases. Depression and anxiety were more common among postmenopause women. Furthermore, there were no differences between the groups regarding the frequency of certain levels of stress among menopause and postmenopause. The multivariate regression analyses revealed that age in years, diastolic BP, consanguinity, regular exercise were a predictor for depression. Meanwhile, diastolic BP, occupation, and physical activity considered the main risk factors for anxiety. Furthermore, age in years, occupation

25. 'HANGAR SHEDS TRUSSES DETAILS; ARCHITECTURAL PLANS ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

25. 'HANGAR SHEDS - TRUSSES - DETAILS; ARCHITECTURAL PLANS - PLANT AREA; MODIFICATION CENTER NO. 1, DAGGETT, CALIFORNIA.' Sections and details of trusses, ironwork, and joints, as modified to show ridge joint detail. As built. This blueline also shows the fire suppression system, added in orange pencil for 'Project 13: Bldgs. T-30, T-50, T-70, T-90' at a later, unspecified date. Contract no. W509 Eng. 2743; File no. 555/84, revision B, dated August 24, 1942. No sheet number. - Barstow-Daggett Airport, Hangar Shed No. 4, 39500 National Trails Highway, Daggett, San Bernardino County, CA

34. Oakland Port and General Depot. Transit Shed No. 7, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

34. Oakland Port and General Depot. Transit Shed No. 7, 936 feet, Building 127 70'0' TRUSS. Sheet 7 of 16 - Oakland Army Base, Transit Shed, East of Dunkirk Street & South of Burma Road, Oakland, Alameda County, CA

10. WIDE GENERAL VIEW OF SHED SHOWING SOUTHWEST FACADE AND ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

10. WIDE GENERAL VIEW OF SHED SHOWING SOUTHWEST FACADE AND TRUCK PLATFORM/STAGING AREA AT SOUTHWEST END OF BUILDING, LOOKING NORTHWEST - Oakland Army Base, Transit Shed, East of Dunkirk Street & South of Burma Road, Oakland, Alameda County, CA

32. Oakland Port and General Depot. Transit Shed No. 7, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

32. Oakland Port and General Depot. Transit Shed No. 7, 936 feet, Building 161 TYPICAL SECTION & DETAILS. Sheet 5 of 16 - Oakland Army Base, Transit Shed, East of Dunkirk Street & South of Burma Road, Oakland, Alameda County, CA

35. Oakland Port and General Depot. Transit Shed No. 7, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

35. Oakland Port and General Depot. Transit Shed No. 7, 936 feet, Building 127 END WALL FRAMING. Sheet 9 of 16 - Oakland Army Base, Transit Shed, East of Dunkirk Street & South of Burma Road, Oakland, Alameda County, CA

Counter and Complicit Masculine Discourse Among Men’s Shed Members

PubMed Central

Mackenzie, Corey S.; Roger, Kerstin; Robertson, Steve; Oliffe, John L.; Nurmi, Mary Anne; Urquhart, James

2017-01-01

Men’s Sheds is a growing international movement aimed at providing men with places and activities that facilitate social connectedness. Despite Men’s Sheds’ focus on males, little attention has been paid to masculinities within the specific context of these settings. The current study used a gender relations framework to explore the ways in which attendees discussed Men’s Sheds, with particular attention to discussions that were complicit and counter to traditional, hegemonic views of masculinity, and diverse positions in between these binaries. The data consisted of transcripts and field notes from four focus groups comprising mostly older, White, retired male members of a Canadian shed (N = 22). The analysis revealed three overall themes: (1) focus on work, (2) independence, and (3) need for male-focused spaces. These themes and associated subthemes suggest that shed members ascribe to dominant masculine values and ideals, but also support more fluid and flexible views of masculinity. Implications are discussed for how working with an array of masculinities within the Men’s Sheds movement will be helpful with respect to their future growth in Canada and internationally. PMID:28068851

Protoearth mass shedding and the origin of the moon

NASA Technical Reports Server (NTRS)

Boss, A. P.

1986-01-01

Darwin's (1980) theory of lunar formation from the earth by means of a rotationally driven dynamic fission instability is presently considered in view of viscous shear's maintenance of solid body rotation throughout the protoearth's accretion phase. Assuming the appropriateness of a polytropic account of the protoearth, it is unlikely that dynamic fission could have occurred; instantaneous spin-up following a giant impact would instead have led to mass shedding. The dynamical phenomenon of mass shedding is here explored on the basis of numerical models for a self-gravitating, axisymmetric, polytropic and dissipative protoearth. It is concluded that mass shedding from the protoearth mantle after a giant impact and explosion could have contributed substantial matter to a lunar disk.

GeneLab: Open Science For Exploration

NASA Technical Reports Server (NTRS)

Galazka, Jonathan

2018-01-01

The NASA GeneLab project capitalizes on multi-omic technologies to maximize the return on spaceflight experiments. The GeneLab project houses spaceflight and spaceflight-relevant multi-omics data in a publicly accessible data commons, and collaborates with NASA-funded principal investigators to maximize the omics data from spaceflight and spaceflight-relevant experiments. I will discuss the current status of GeneLab and give specific examples of how the GeneLab data system has been used to gain insight into how biology responds to spaceflight conditions.

A Museum Learning Lab

ERIC Educational Resources Information Center

Vandiver, Kathleen M.; Bijur, Jon Markowitz; Epstein, Ari W.; Rosenthal, Beryl; Stidsen, Don

2008-01-01

The "Learning Lab: The Cell" exhibit was developed by the Massachusetts Institute of Technology (MIT) Museum and the MIT Center for Environmental Health Sciences (CEHS). Specially designed for middle and high school students, the Learning Lab provides museum visitors of all ages with fascinating insights into how our living cells work. The…

Linear Regression between CIE-Lab Color Parameters and Organic Matter in Soils of Tea Plantations

NASA Astrophysics Data System (ADS)

Chen, Yonggen; Zhang, Min; Fan, Dongmei; Fan, Kai; Wang, Xiaochang

2018-02-01

To quantify the relationship between the soil organic matter and color parameters using the CIE-Lab system, 62 soil samples (0-10 cm, Ferralic Acrisols) from tea plantations were collected from southern China. After air-drying and sieving, numerical color information and reflectance spectra of soil samples were measured under laboratory conditions using an UltraScan VIS (HunterLab) spectrophotometer equipped with CIE-Lab color models. We found that soil total organic carbon (TOC) and nitrogen (TN) contents were negatively correlated with the L* value (lightness) ( r = -0.84 and -0.80, respectively), a* value (correlation coefficient r = -0.51 and -0.46, respectively) and b* value ( r = -0.76 and -0.70, respectively). There were also linear regressions between TOC and TN contents with the L* value and b* value. Results showed that color parameters from a spectrophotometer equipped with CIE-Lab color models can predict TOC contents well for soils in tea plantations. The linear regression model between color values and soil organic carbon contents showed it can be used as a rapid, cost-effective method to evaluate content of soil organic matter in Chinese tea plantations.

19. RW Meyer Sugar: 18761889. Cooling Shed Interior, 1881. View: ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

19. RW Meyer Sugar: 1876-1889. Cooling Shed Interior, 1881. View: Looking toward west end of cooling shed. After the concentrated syrup flowed out of the sorghum pan it cooled and crystallized in large sugar coolers. The humidity and vapors caused by the sorghum pan would have retarded the crystallizing and cooling of the sugar in the boiling house. In 1881 this shed was constructed to house the coolers and the sugar before it was dried in the centrifugals. - R. W. Meyer Sugar Mill, State Route 47, Kualapuu, Maui County, HI

30. Oakland Port and General Depot. Transit Shed No. 7, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

30. Oakland Port and General Depot. Transit Shed No. 7, 936 feet, Building 161 PLOT PLAN & TRANSVERSE SECTION. Sheet 1 of 16 - Oakland Army Base, Transit Shed, East of Dunkirk Street & South of Burma Road, Oakland, Alameda County, CA

33. Oakland Port and General Depot. Transit Shed No. 7, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

33. Oakland Port and General Depot. Transit Shed No. 7, 936 feet, Building 127 STAIR & TOILET ROOM DETAILS. Sheet 6 of 16 - Oakland Army Base, Transit Shed, East of Dunkirk Street & South of Burma Road, Oakland, Alameda County, CA

31. Oakland Port and General Depot. Transit Shed No. 7, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

31. Oakland Port and General Depot. Transit Shed No. 7, 936 feet, Building 127 STAIR & TOILET ROOM DETAILS. Sheet 3 of 16 - Oakland Army Base, Transit Shed, East of Dunkirk Street & South of Burma Road, Oakland, Alameda County, CA

5. PERSONNEL ROOM ON WEST SIDE OF PYROTECHNIC SHED (BLDG. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

5. PERSONNEL ROOM ON WEST SIDE OF PYROTECHNIC SHED (BLDG. 757) STORAGE LOCKER ON EAST WALL; PADDED TABLE ON SOUTH WALL. - Vandenberg Air Force Base, Space Launch Complex 3, Pyrotechnic Shed, Napa & Alden Roads, Lompoc, Santa Barbara County, CA

Studies of Human 2,4-Dienoyl CoA Reductase Shed New Light on Peroxisomal β-Oxidation of Unsaturated Fatty Acids

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hua, Tian; Wu, Dong; Ding, Wei

2012-10-15

Peroxisomes play an essential role in maintaining fatty acid homeostasis. Although mitochondria are also known to participate in the catabolism of fatty acids via β-oxidation, differences exist between the peroxisomal and mitochondrial β-oxidation. Only peroxisomes, but not mitochondrion, can shorten very long chain fatty acids. Here, we describe the crystal structure of a ternary complex of peroxisomal 2,4-dienoyl CoA reductases (pDCR) with hexadienoyl CoA and NADP, as a prototype for comparison with the mitochondrial 2,4-dienoyl CoA reductase (mDCR) to shed light on the differences between the enzymes from the two organelles at the molecular level. Unexpectedly, the structure of pDCRmore » refined to 1.84 Å resolution reveals the absence of the tyrosine-serine pair seen in the active site of mDCR, which together with a lysine and an asparagine have been deemed a hallmark of the SDR family of enzymes. Instead, aspartate hydrogen-bonded to the Cα hydroxyl via a water molecule seems to perturb the water molecule for protonation of the substrate. Our studies provide the first structural evidence for participation of water in the DCR-catalyzed reactions. Biochemical studies and structural analysis suggest that pDCRs can catalyze the shortening of six-carbon-long substrates in vitro. However, the Km values of pDCR for short chain acyl CoAs are at least 6-fold higher than those for substrates with 10 or more aliphatic carbons. Unlike mDCR, hinge movements permit pDCR to process very long chain polyunsaturated fatty acids.« less

27. GENERAL INTERIOR VIEW SHOWING SOUTH CORNER OF SHED WITH ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

27. GENERAL INTERIOR VIEW SHOWING SOUTH CORNER OF SHED WITH ONE-STORY OFFICES, SHOWING TYPICAL COLUMN BASE WITH TIMBER BOLTED TO STEEL 'L' SHOE - Oakland Army Base, Transit Shed, East of Dunkirk Street & South of Burma Road, Oakland, Alameda County, CA

Lab-on a-Chip

NASA Technical Reports Server (NTRS)

2003-01-01

Helen Cole, the project manager for the Lab-on-a-Chip Applications Development program, and Lisa Monaco, the project scientist for the program, insert a lab on a chip into the Caliper 42 which is specialized equipment that controls processes on commercial chips to support development of lab-on-a-chip applications. The system has special microscopes and imaging systems, so scientists can process and study different types of fluid, chemical, and medical tests conducted on chips. For example, researchers have examined fluorescent bacteria as it flows through the chips' fluid channels or microfluidic capillaries. Researchers at NASA's Marshall Space Flight Center (MSFC) in Huntsville, Alabama, have been studying how the lab-on-a-chip technology can be used for microbial detection, water quality monitoring, and detecting biosignatures of past or present life on Mars. The Marshall Center team is also collaborating with scientists at other NASA centers and at universities to develop custom chip designs for not only space applications, but for many Earth applications, such as for detecting deadly microbes in heating and air systems. (NASA/MSFC/D.Stoffer)

New Directions in X-Ray Light Sources

ScienceCinema

Falcone, Roger

2017-12-09

July 15, 2008 Berkeley Lab lecture: Molecular movies of chemical reactions and material phase transformations need a strobe of x-rays, the penetrating light that reveals how atoms and molecules assemble in chemical and biological systems and complex materials. Roger Falcone, Director of the Advanced Light Source,will discuss a new generation of x ray sources that will enable a new science of atomic dynamics on ultrafast timescales.

Afterglow Observations Shed New Light on the Nature of X-ray Flashes

DOE Office of Scientific and Technical Information (OSTI.GOV)

Granot, J

X-ray flashes (XRFs) and X-ray rich gamma-ray bursts (XRGRBs) share many observational characteristics with long duration ({approx}> 2 s) GRBs, but the reason for which the spectral energy distribution of their prompt emission peaks at lower photon energies, E{sub p}, is still a subject of debate. Although many different models have been invoked in order to explain the lower values of E{sub p}, their implications for the afterglow emission were not considered in most cases, mainly because observations of XRF afterglows have become available only recently. Here we examine the predictions of the various XRF models for the afterglow emission,more » and test them against the observations of XRF 030723 and XRGRB 041006, the events with the best monitored afterglow light curves in their respective class. We show that most existing XRF models are hard to reconcile with the observed afterglow light curves, which are very flat at early times. Such light curves are, however, naturally produced by a roughly uniform jet with relatively sharp edges that is viewed off-axis (i.e. from outside of the jet aperture). This type of model self consistently accommodates both the observed prompt emission and the afterglow light curves of XRGRB 041006 and XRF 030723, implying viewing angles {theta}{sub obs} from the jet axis of ({theta}{sub obs}-{theta}{sub 0}) {approx} 0.15 {theta}{sub 0} and ({theta}{sub obs}-{theta}{sub 0}) {approx} {theta}{sub 0}, respectively, where {theta}{sub 0} {approx} 3{sup o} is the half-opening angle of the jet. This suggests that GRBs, XRGRBs and XRFs are intrinsically similar relativistic jets viewed from different angles. It is then natural to identify GRBs with {gamma}({theta}{sub obs} - {theta}{sub 0}) {approx}< 1, XRGRBs with 1 {approx}< ({theta}{sub obs} - {theta}{sub 0}) {approx}< a few, and XRFs with {gamma}({theta}{sub obs} - {theta}{sub 0}) {approx}> a few, where {gamma} is the Lorentz factor of the outflow near the edge of the jet from which most

Two paths from lab to market: Product and standard

NASA Astrophysics Data System (ADS)

Knapp, Robert H.

2018-01-01

To shed light on the movement of sustainable technologies from basic science to widespread use, this chapter describes key aspects of the quite different paths followed by two important examples—photovoltaics (a product) and passive-house buildings (a standard). Discussion of photovoltaics includes the experience curve concept, the increasing significance of balance-of-system costs, and the importance of market heterogeneity (niches and sub-national markets) to the long-term trajectory of major cost reductions. Discussion of passive-houses highlights the array of technical developments needed for present-day energy efficient houses, and the relevance of "stretch" standards to the development of a market for very high-performance houses.

ERLN Lab Compendium Fact Sheet

EPA Pesticide Factsheets

The Compendium is an online database of environmental testing laboratories nationwide. It enables labs to create profiles of their capabilities, so emergency responders can quickly identify a lab that will meet their support needs.

Development of cyclic shedding teeth from semi-shedding teeth: the inner dental arcade of the stem osteichthyan Lophosteus

NASA Astrophysics Data System (ADS)

Chen, Donglei; Blom, Henning; Sanchez, Sophie; Tafforeau, Paul; Märss, Tiiu; Ahlberg, Per E.

2017-05-01

The numerous cushion-shaped tooth-bearing plates attributed to the stem group osteichthyan Lophosteus superbus, which are argued here to represent an early form of the osteichthyan inner dental arcade, display a previously unknown and presumably primitive mode of tooth shedding by basal hard tissue resorption. They carry regularly spaced, recumbent, gently recurved teeth arranged in transverse tooth files that diverge towards the lingual margin of the cushion. Three-dimensional reconstruction from propagation phase-contrast synchrotron microtomography (PPC-SRµCT) reveals remnants of the first-generation teeth embedded in the basal plate, a feature never previously observed in any taxon. These teeth were shed by semi-basal resorption with the periphery of their bases retained as dentine rings. The rings are highly overlapped, which evidences tooth shedding prior to adding the next first-generation tooth at the growing edge of the plate. The first generation of teeth is thus diachronous. Successor teeth at the same sites underwent cyclical replacing and shedding through basal resorption, producing stacks of buried resorption surfaces separated by bone of attachment. The number and spatial arrangement of resorption surfaces elucidates that basal resorption of replacement teeth had taken place at the older tooth sites before the addition of the youngest first-generation teeth at the lingual margin. Thus, the replacement tooth buds cannot have been generated by a single permanent dental lamina at the lingual edge of the tooth cushion, but must have arisen either from successional dental laminae associated with the individual predecessor teeth, or directly from the dental epithelium of these teeth. The virtual histological dissection of these Late Silurian microfossils broadens our understanding of the development of the gnathostome dental systems and the acquisition of the osteichthyan-type of tooth replacement.

Physics Labs with Flavor

ERIC Educational Resources Information Center

Agrest, Mikhail M.

2009-01-01

This paper describes my attempts to look deeper into the so-called "shoot for your grade" labs, started in the '90s, when I began applying my teaching experience in Russia to introductory physics labs at the College of Charleston and other higher education institutions in South Carolina. The term "shoot for your grade" became popular among…

Making Real Virtual Labs

ERIC Educational Resources Information Center

Keller, Harry E.; Keller, Edward E.

2005-01-01

Francis Bacon began defining scientific methodology in the early 17th century, and secondary school science classes began to implement science labs in the mid-19th century. By the early 20th century, leading educators were suggesting that science labs be used to develop scientific thinking habits in young students, and at the beginning of the 21st…

NOT Another Lab Report

ERIC Educational Resources Information Center

Ende, Fred

2012-01-01

Ask students to name the aspects of science class they enjoy most, and working on labs will undoubtedly be mentioned. What often won't be included, however, is writing lab reports. For many students, the process of exploration and data collection is paramount, while the explanation and analysis of findings often takes a backseat. After all, if…

Are Virtual Labs as Effective as Hands-on Labs for Undergraduate Physics? A Comparative Study at Two Major Universities

ERIC Educational Resources Information Center

Darrah, Marjorie; Humbert, Roxann; Finstein, Jeanne; Simon, Marllin; Hopkins, John

2014-01-01

Most physics professors would agree that the lab experiences students have in introductory physics are central to the learning of the concepts in the course. It is also true that these physics labs require time and money for upkeep, not to mention the hours spent setting up and taking down labs. Virtual physics lab experiences can provide an…

The Development of MSFC Usability Lab

NASA Technical Reports Server (NTRS)

Cheng, Yiwei; Richardson, Sally

2010-01-01

This conference poster reviews the development of the usability lab at Marshall Space Flight Center. The purpose of the lab was to integrate a fully functioning usability laboratory to provide a resource for future human factor assessments. and to implement preliminary usability testing on a MSFC website to validate the functionality of the lab.

The Transition from Thick to Thin Plate Wake Physics: Whither Vortex Shedding?

NASA Technical Reports Server (NTRS)

Rai, Man Mohan

2016-01-01

The near and very near wake of a flat plate with a circular trailing edge is investigated with data from direct numerical simulations. Computations were performed for six different combinations of the Reynolds numbers based on plate thickness (D) and boundary layer momentum thickness upstream of the trailing edge (theta). Unlike the case of the cylinder, these Reynolds numbers are independent parameters for the flat plate. The separating boundary layers are turbulent in all the cases investigated. One objective of the study is to understand the changes in the wake vortex shedding process as the plate thickness is reduced (increasing theta/D). The value of D varies by a factor of 16 and that of theta by approximately 5 in the computations. Vortex shedding is vigorous in the low theta/D cases with a substantial decrease in shedding intensity in the large theta/D cases. Other shedding characteristics are also significantly altered with increasing theta/D. A visualization of the shedding process in the different cases is provided and discussed. The basic shedding mechanism is explored in depth. The effect of changing theta/D on the time-averaged, near-wake velocity statistics is also discussed. A functional relationship between the shedding frequency and the Reynolds numbers mentioned above is obtained.

Shedding Synchrotron Light on a Puzzle of Glasses

ScienceCinema

Chumakov, Aleksandr [European Synchrotron Radiation Facility, Grenoble, France

2017-12-09

Vibrational dynamics of glasses remains a point of controversial discussions. In particular, the density of vibrational states (DOS) reveals an excess of states above the Debye model called "boson peak." Despite the fact that this universal feature for all glasses has been known for more than 35 years, the nature of the boson peak is still not understood. The application of nuclear inelastic scattering via synchrotron radiation perhaps provides a clearer, more consistent picture of the subject. The distinguishing features of nuclear inelastic scattering relative to, e.g., neutron inelastic scattering, are ideal momentum integration and exact scaling of the DOS in absolute units. This allows for reliable comparison to data from other techniques such as Brillouin light scattering. Another strong point is ideal isotope selectivity: the DOS is measured for a single isotope with a specific low-energy nuclear transition. This allows for special "design" of an experiment to study, for instance, the dynamics of only center-of-mass motions. Recently, we have investigated the transformation of the DOS as a function of several key parameters such as temperature, cooling rate, and density. In all cases the transformation of the DOS is sufficiently well described by a transformation of the continuous medium, in particular, by changes of the macroscopic density and the sound velocity. These results suggest a collective sound-like nature of vibrational dynamics in glasses and cast doubts on microscopic models of glass dynamics. Further insight can be obtained in combined studies of glass with nuclear inelastic and inelastic neutron scattering. Applying two techniques, we have measured the energy dependence of the characteristic correlation length of atomic motions. The data do not reveal localization of atomic vibrations at the energy of the boson peak. Once again, the results suggest that special features of glass dynamics are related to extended motions and not to local models.

Berkeley Lab - Materials Sciences Division

Science.gov Websites

Berkeley Lab Berkeley Lab A-Z Index Phone Book Jobs Search DOE Search MSD Go MSD - Materials Investigators Division Staff Facilities and Centers Staff Jobs Safety Personnel Resources Committees In Case of

Detail of old rain shed (Building No. 43) showing vertical ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Detail of old rain shed (Building No. 43) showing vertical posts. Note rock foundations of wood tanks once located under the rain shed on the ground at center of photograph. - Hawaii Volcanoes National Park Water Collection System, Hawaii Volcanoes National Park, Volcano, Hawaii County, HI

Men's Sheds function and philosophy: towards a framework for future research and men's health promotion.

PubMed

Wilson, Nathan J; Cordier, Reinie; Doma, Kenji; Misan, Gary; Vaz, Sharmila

2015-08-01

The Men's Shed movement supports a range of men's health promotion initiatives. This paper examines whether a Men's Shed typology could inform future research and enable more efficient and targeted health promotion activities through Men's Sheds. The International Men's Shed Survey consisted of a cross-sectional exploration of sheds, their members, and health and social activities. Survey data about shed 'function' and 'philosophy' were analysed using descriptive and inferential statistics. A framework of Men's Sheds based on function and philosophy demonstrated that most sheds serve a primary utility function, a secondary social function, but most importantly a primary social opportunity philosophy. Sheds with a primary health philosophy participated in fewer health promotion activities when compared with sheds without a primary health philosophy. In addition to the uniform health promotion resources distributed by the Men's Shed associations, specific health promotion activities, such as prostate education, are being initiated from an individual shed level. This framework can potentially be used to enable future research and health promotion activities to be more efficiently and effectively targeted. SO WHAT? Men experience poorer health and well being outcomes than women. This framework offers a novel approach to providing targeted health promotion activities to men in an environment where it is okay to talk about men's health.

Influence of particle shedding from silicone tubing on antibody stability.

PubMed

Saller, Verena; Hediger, Constanze; Matilainen, Julia; Grauschopf, Ulla; Bechtold-Peters, Karoline; Mahler, Hanns-Christian; Friess, Wolfgang

2018-05-01

Peristaltic pumps are increasingly employed during fill & finish operations of a biopharmaceutical drug, due to sensitivity of many biological products to rotary piston pump-related stresses. Yet, possibly also unit operations using peristaltic pumps may shed particulates into the final product due to abrasion from the employed tubing. It was the aim of this study to elucidate the potential influence of particles shed from peristaltic pump tubing on the stability of a drug product. Spiking solutions containing shed silicone particles were prepared via peristaltic pumping of placebo under recirculating conditions and subsequently characterized. Two formulated antibodies were spiked with two realistic, but worst-case levels of particles and a 6-month accelerated stability study with storage at 2-8, 25 and 40°C were conducted. Regarding the formation of aggregates and fragments, both mAbs degraded at their typically expected rates and no additional impact of spiked particles was observed. No changes were discerned however in turbidity, subvisible and visible particle assessments. Flow imaging data for one of the mAb formulations with spiked particles suggested limited colloidal stability of shed particles as indicated by a similar increase in spiked placebo. Shed silicone particles from peristaltic pump tubing are assumed to not impair drug product stability. © 2016 Royal Pharmaceutical Society.

Labs That Are a Blast.

ERIC Educational Resources Information Center

Hendrix, Laura

1996-01-01

Presents activities that use a simple homemade apparatus called "the cannon" to demonstrate Newton's Third Law. Reviews the chemistry concepts behind the ignition of the cannon and presents the Momentum Lab and the Projectile Motion Lab. (JRH)

Vortex shedding flow meter performance at high flow velocities

NASA Technical Reports Server (NTRS)

Siegwarth, J. D.

1986-01-01

In some of the ducts of the Space Shuttle Main Engine (SSME), the maximum liquid oxygen flow velocities approach 10 times those at which liquid flow measurements are normally made. The hydrogen gas flow velocities in other ducts exceed the maximum for gas flow measurement by more than a factor of 3. The results presented here show from water flow tests that vortex shedding flow meters of the appropriate design can measure water flow to velocities in excess of 55 m/s, which is a Reynolds number of about 2 million. Air flow tests have shown that the same meter can measure flow to a Reynolds number of at least 22 million. Vortex shedding meters were installed in two of the SSME ducts and tested with water flow. Narrow spectrum lines were obtained and the meter output frequencies were proportional to flow to + or - 0.5% or better over the test range with no flow conditioning, even though the ducts had multiple bends preceeding the meter location. Meters with the shedding elements only partially spanning the pipe and some meters with ring shaped shedding elements were also tested.

An intelligent load shedding scheme using neural networks and neuro-fuzzy.

PubMed

Haidar, Ahmed M A; Mohamed, Azah; Al-Dabbagh, Majid; Hussain, Aini; Masoum, Mohammad

2009-12-01

Load shedding is some of the essential requirement for maintaining security of modern power systems, particularly in competitive energy markets. This paper proposes an intelligent scheme for fast and accurate load shedding using neural networks for predicting the possible loss of load at the early stage and neuro-fuzzy for determining the amount of load shed in order to avoid a cascading outage. A large scale electrical power system has been considered to validate the performance of the proposed technique in determining the amount of load shed. The proposed techniques can provide tools for improving the reliability and continuity of power supply. This was confirmed by the results obtained in this research of which sample results are given in this paper.

RoboLab and virtual environments

NASA Technical Reports Server (NTRS)

Giarratano, Joseph C.

1994-01-01

A useful adjunct to the manned space station would be a self-contained free-flying laboratory (RoboLab). This laboratory would have a robot operated under telepresence from the space station or ground. Long duration experiments aboard RoboLab could be performed by astronauts or scientists using telepresence to operate equipment and perform experiments. Operating the lab by telepresence would eliminate the need for life support such as food, water and air. The robot would be capable of motion in three dimensions, have binocular vision TV cameras, and two arms with manipulators to simulate hands. The robot would move along a two-dimensional grid and have a rotating, telescoping periscope section for extension in the third dimension. The remote operator would wear a virtual reality type headset to allow the superposition of computer displays over the real-time video of the lab. The operators would wear exoskeleton type arms to facilitate the movement of objects and equipment operation. The combination of video displays, motion, and the exoskeleton arms would provide a high degree of telepresence, especially for novice users such as scientists doing short-term experiments. The RoboLab could be resupplied and samples removed on other space shuttle flights. A self-contained RoboLab module would be designed to fit within the cargo bay of the space shuttle. Different modules could be designed for specific applications, i.e., crystal-growing, medicine, life sciences, chemistry, etc. This paper describes a RoboLab simulation using virtual reality (VR). VR provides an ideal simulation of telepresence before the actual robot and laboratory modules are constructed. The easy simulation of different telepresence designs will produce a highly optimum design before construction rather than the more expensive and time consuming hardware changes afterwards.

Porphyromonas gingivalis-mediated shedding of extracellular matrix metalloproteinase inducer (EMMPRIN) by oral epithelial cells: a potential role in inflammatory periodontal disease.

PubMed

Feldman, Mark; La, Vu Dang; Lombardo Bedran, Telma Blanca; Palomari Spolidorio, Denise Madalena; Grenier, Daniel

2011-12-01

Extracellular matrix metalloproteinase inducer (EMMPRIN) or CD147 is a transmembrane glycoprotein expressed by various cell types, including oral epithelial cells. Recent studies have brought evidence that EMMPRIN plays a role in periodontitis. In the present study, we investigated the effect of Porphyromonas gingivalis, a major pathogen in chronic periodontitis, on the shedding of membrane-anchored EMMPRIN and on the expression of the EMMPRIN gene by oral epithelial cells. A potential contribution of shed EMMPRIN to the inflammatory process of periodontitis was analyzed by evaluating the effect of recombinant EMMPRIN on cytokine and matrix metalloproteinase (MMP) secretion by human gingival fibroblasts. ELISA and immunofluorescence analyses revealed that P. gingivalis mediated the shedding of epithelial cell-surface EMMPRIN in a dose- and time-dependent manner. Cysteine proteinase (gingipain)-deficient P. gingivalis mutants were used to demonstrate that both Arg- and Lys-gingipain activities are involved in EMMPRIN shedding. Real-time PCR showed that P. gingivalis had no significant effect on the expression of the EMMPRIN gene in epithelial cells. Recombinant EMMPRIN induced the secretion of IL-6 and MMP-3 by gingival fibroblasts, a phenomenon that appears to involve mitogen activated protein kinases. The present study brought to light a new mechanism by which P. gingivalis can promote the inflammatory response during periodontitis. Copyright © 2011 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.

Virtual Labs and Virtual Worlds

NASA Astrophysics Data System (ADS)

Boehler, Ted

2006-12-01

Virtual Labs and Virtual Worlds Coastline Community College has under development several virtual lab simulations and activities that range from biology, to language labs, to virtual discussion environments. Imagine a virtual world that students enter online, by logging onto their computer from home or anywhere they have web access. Upon entering this world they select a personalized identity represented by a digitized character (avatar) that can freely move about, interact with the environment, and communicate with other characters. In these virtual worlds, buildings, gathering places, conference rooms, labs, science rooms, and a variety of other “real world” elements are evident. When characters move about and encounter other people (players) they may freely communicate. They can examine things, manipulate objects, read signs, watch video clips, hear sounds, and jump to other locations. Goals of critical thinking, social interaction, peer collaboration, group support, and enhanced learning can be achieved in surprising new ways with this innovative approach to peer-to-peer communication in a virtual discussion world. In this presentation, short demos will be given of several online learning environments including a virtual biology lab, a marine science module, a Spanish lab, and a virtual discussion world. Coastline College has been a leader in the development of distance learning and media-based education for nearly 30 years and currently offers courses through PDA, Internet, DVD, CD-ROM, TV, and Videoconferencing technologies. Its distance learning program serves over 20,000 students every year. sponsor Jerry Meisner

Genetic relationship between wool shedding in ewe-lambs and ewes

USDA-ARS?s Scientific Manuscript database

Interest in reducing labor costs related to shearing has led to the development of breeds that naturally shed their wool annually. This goal has been achieved by introducing hair-sheep genetics. These developments are relatively recent and thus the genetic underpinnings of wool shedding (WS) are not...

Looking north through the C.W.E. Storage Shed (Bldg. 126) ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Looking north through the C.W.E. Storage Shed (Bldg. 126) - Atchison, Topeka, Santa Fe Railroad, Albuquerque Shops, C.W.E. Storage Shed, 908 Second Street, Southwest, Albuquerque, Bernalillo County, NM

Shedding Light on Hexaquarks

NASA Astrophysics Data System (ADS)

Bashkanov, M.; Skorodko, T.; Clement, H.; Watts, D. P.

Several new findings in the four, five and six quark systems reheat the interest in the field of multiquark states (beyond the trivial qq¯ and qqq). A lot of progress has recently been made in the 6q sector, on both the theoretical and experimental side. A resonance like structure observed in double-pionic fusion to the deuteron, at M = 2.38 GeV with Γ = 70 MeV and I(JP) = 0(3+) has been consistently observed in a wealth of reaction channels, supporting the existence of a resonant dibaryon state - the d∗(2380). These studies include measurement of all the principle strong decay channels in pn collisions in the quasifree mode by the WASA-at-COSY and HADES collaborations. The internal structure of the d∗(2380) is largely unknown. It can contain various ”hidden color” 6q configurations, ΔΔ molecular states with angular momentum L = 0,2,4,6 as well as meson-assisted dressed dibaryon structures. The large set of experimental data obtained to date gives some constraints on the internal structure of the d∗(2380) dibaryon, but does not settle the issue. The d∗ is the only multiquark state which can be produced copiously at current facilities, offering unique access to information beyond its basic quantum numbers, particularly its physical size and internal structure.

Playing with Light: Adventures in Optics and Spectroscopy for Honors and Majors General Chemistry

ERIC Educational Resources Information Center

van Staveren, Marie N.; Edwards, Kimberly D.; Apkarian, V. A.

2012-01-01

A lab was developed for use in an undergraduate honors and majors general chemistry laboratory to introduce students to optics, spectroscopy, and the underlying principles of quantum mechanics. This lab includes four mini-experiments exploring total internal reflection, the tunneling of light, spectra of sparklers and colored candles, and emission…

GeneLab

NASA Technical Reports Server (NTRS)

Berrios, Daniel C.; Thompson, Terri G.

2015-01-01

NASA GeneLab is expected to capture and distribute omics data and experimental and process conditions most relevant to research community in their statistical and theoretical analysis of NASAs omics data.

Incidence of Latent Virus Shedding during Space Flight

NASA Technical Reports Server (NTRS)

Mehta, Satish K.; Cohrs, Randall J.; Gilden, Donald H.; Tyring, Stephen K.; Ott, C. Mark; Pierson, Duane L.

2008-01-01

Measurements of immune parameters of both cellular and innate immunity indicate alterations in immune function in astronauts. Immune changes are due to stress and perhaps other factors associated with launch, flight, and landing phases. Medical relevance of observed changes is not known. The reactivation of latent viruses has been identified as an important in vivo indicator of clinically relevant immune changes. The polymerase chain reaction (PCR) was used to detect the presence of specific viral DNA in body fluids. Initial studies demonstrated Epstein-Barr virus (EBV) reactivation during all 3 mission phases. EBV is shed in saliva following reactivation from B-cells. Incidence of EBV in saliva was higher than control subjects during all 3 mission phases. However, quantitative PCR revealed 10-fold higher levels of EBV DNA present in saliva collected during flight than found in pre- and post flight specimens. To determine if other latent viruses showed similar effects, cytomegalovirus (CMV), another herpes virus, shed in urine following reactivation was studied. A very low incidence (less than 2%) of CMV in urine is found in healthy, lowstressed individuals. However, 25-50% of astronauts shed CMV in their urine before, during, or after flight. Our studies are now focused on varicella-zoster virus (VZV), the etiological agent of chicken-pox during childhood and shingles later in life. We demonstrated reactivation of VZV and shedding of the virus during and after spaceflight in saliva of astronauts with no sign of active infection or symptoms. The maximum shedding of VZV occurred during the flight phase and diminishes rapidly during the first five days after landing. We have utilized the same PCR assay for VZV in a clinical study of shingles patients. Generally, shingles patients shed much more VZV in saliva than astronauts. However, the VZV levels in astronauts overlap with the lower range of VZV numbers in shingles patients. Saliva from shingles patients and

Sensitivity of digital dental photo CIE L*a*b* analysis compared to spectrophotometer clinical assessments over 6 months.

PubMed

Sluzker, Ariel; Knösel, Michael; Athanasiou, Athanasios E

2011-10-01

To assess the sensitivity of digital dental photo CIE L*a*b* analysis compared to clinical spectrophotometer assessments over 6 months. CIE L*a*b* values for the upper right central incisors of 14 predoctoral dental students subjected to certain color-relevant exclusion criteria were recorded at baseline (T0), after 6 months (T1), and 1 week later (T2), using (Method 1) a spectrophotometer and (Method 2) a method of digital photo analysis. Statistical analysis of color and lightness data between both methods and time points were assessed using the Shapiro-Wilk test, Pearson's correlation coefficient (r), Dahlberg's formula for method error calculation, and paired samples t-tests, adopting a level of significance alpha = 0.05. Between T0 - T1, the spectrophotometer recorded significant changes in lightness (75.51 > 77.75) and color values (a*: 3.25 > 2.38; b*: 18.47 > 17.31), whereas significant changes with Method 2 were only seen for b* (21.51 > 20.57). No significant changes for overall color and lightness changes deltaE to deltaE2 were found for either of the methods. The error of the method (T1-T2) and corresponding correlation coefficients r for values L*a*b* were found to be 1.44 / 0.43 / 0.62 (r: 0.69; P = 0.007/0.64; P = 0.14/0.9; P < 0.001) for Method 1 and 0.97/0.67/1.25 (r : 0.87; P < 0.001/0.63; P = 0.17/0.57, P = 0.04) for Method 2, respectively.

Coherent structures shed by multiscale cut-in trailing edge serrations on lifting wings

NASA Astrophysics Data System (ADS)

Prigent, S. L.; Buxton, O. R. H.; Bruce, P. J. K.

2017-07-01

This experimental study presents the effect of multiscale cut-in trailing edge serrations on the coherent structures shed into the wake of a lifting wing. Two-probe span-wise hot-wire traverses are performed to study spectra, coherence, and phase shift. In addition, planar particle image velocimetry is used to study the spatio-temporal structure of the vortices shed by the airfoils. Compared with a single tone sinusoidal serration, the multiscale ones reduce the vortex shedding energy as well as the span-wise coherence. Results indicate that the vortex shedding is locked into an arch-shaped cell structure. This structure is weakened by the multiscale patterns, which explains the reduction in both shedding energy and coherence.

Awakening interest in the natural sciences - BASF's Kids' Labs.

PubMed

Lang, Cinthia

2012-01-01

At BASF's Ludwigshafen headquarters, kids and young adults in grades 1-13 can learn about chemistry in the Kids' Labs. Different programs exist for different levels of knowledge. In the two 'Hands-on Lab H(2)O & Co.' Kids' Labs, students from grades 1-6 explore the secrets of chemistry. BASF Kids' Labs have now been set up in over 30 countries. In Switzerland alone, almost 2,000 students have taken part in the 'Water Loves Chemistry' Kids' Lab since it was started in 2011. In Alsace, 600 students have participated to date. In the Teens' Lab 'Xplore Middle School', middle school students explore five different programs with the themes 'substance labyrinth', 'nutrition', 'coffee, caffeine & co.', 'cosmetics' and 'energy'. Biotechnological methods are the focus of the Teens' Lab 'Xplore Biotech' for students taking basic and advanced biology courses. In the 'Xplore High School' Teens' Lab, chemistry teachers present their own experimental lab instruction for students in basic and advanced chemistry courses. The Virtual Lab has been expanding the offerings of the BASF Kids' Labs since 2011. The online lab was developed by the company for the International Year Of Chemistry and gives kids and young adults the opportunity to do interactive experiments outside of the lab.

ADAM15 is involved in MICB shedding and mediates the effects of gemcitabine on MICB shedding in PANC-1 pancreatic cancer cells.

PubMed

Duan, Xiaohui; Mao, Xianhai; Sun, Weijia

2013-03-01

The aim of this study was to investigate the role of ADAM15 in MHC class I polypeptide-related sequence B (MICB) protein ectodomain shedding and observe whether or not gemcitabine affects MICB shedding from PANC-1 cells. In this study, immunohistochemistry of MICB and ADAM15 were performed on tumor samples obtained from 93 patients with pancreatic ductal adenocarcinoma (PDAC). The expression of MICB and ADAM15 in the PDAC tissues was significantly higher compared with that in the normal tissues of the pancreas. Statistical analysis showed a significant correlation between the expression of MICB and certain classic clinicopathological characteristics (i.e., histological grade and TNM stage). ADAM15 expression was found to correlate with lymph node metastasis and TNM stage. The Spearman's rank test suggested that the expression of MICB was inversely correlated with that of ADAM15 in PDAC tissues. Knockdown of ADAM15 in PANC-1 cells clearly upregulated MICB expression on the cellular surface and downregulated soluble MICB (sMICB) levels in the culture supernatants. A non-toxic dose of 0.5 µmol/l gemcitabine suppresses ADAM15 expression leading, at the same time, to an increase in MICB expression and a decrease in sMICB production in PANC-1 cells. The mRNA levels of MICB did not change following PANC-1 exposure to gemcitabine. Further study suggests that the suppressive effect of gemcitabine on MICB shedding in PANC-1 cells is mediated by ADAM15 downregulation. In conclusion, the results of the present study support the hypothesis that ADAM15 is involved in MICB shedding of PANC-1 cells and that gemcitabine inhibits MICB ectodomain shedding through the suppression of ADAM15.

26. VIEW OF METAL SHED OVER SHIELDING TANK WITH CAMERA ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

26. VIEW OF METAL SHED OVER SHIELDING TANK WITH CAMERA FACING SOUTHWEST. SHOWS OPEN SIDE OF SHED ROOF, HERCULON SHEET, AND HAND-OPERATED CRANE. TAKEN IN 1983. INEL PHOTO NUMBER 83-476-2-9, TAKEN IN 1983. PHOTOGRAPHER NOT NAMED. - Idaho National Engineering Laboratory, Advanced Reentry Vehicle Fusing System, Scoville, Butte County, ID

Curricular Adaptations in Introductory Physics Labs

NASA Astrophysics Data System (ADS)

Dreyfus, Benjamin W.; Ewell, Mary; Moore, Kimberly

2017-01-01

When curricular materials are disseminated to new sites, there can be a tension between fidelity to the original intent of the developers and adaptation to local needs. In this case study we look at a lab activity that was initially developed for an introductory physics for the life sciences (IPLS) course at the University of Maryland, then implemented at George Mason University with significant adaptations. The goals of the two implementations were overlapping, but also differed in ways that are reflected in the two versions of the lab. We compare student lab report data from the two sites to examine the impacts of the adaptation on how students engaged with the lab.

Lab architecture

NASA Astrophysics Data System (ADS)

Crease, Robert P.

2008-04-01

There are few more dramatic illustrations of the vicissitudes of laboratory architecturethan the contrast between Building 20 at the Massachusetts Institute of Technology (MIT) and its replacement, the Ray and Maria Stata Center. Building 20 was built hurriedly in 1943 as temporary housing for MIT's famous Rad Lab, the site of wartime radar research, and it remained a productive laboratory space for over half a century. A decade ago it was demolished to make way for the Stata Center, an architecturally striking building designed by Frank Gehry to house MIT's computer science and artificial intelligence labs (above). But in 2004 - just two years after the Stata Center officially opened - the building was criticized for being unsuitable for research and became the subject of still ongoing lawsuits alleging design and construction failures.

Modern Laboratory Exercises in Astronomy V: New Lab Exercises, Electronic Publication and Dissemination

NASA Astrophysics Data System (ADS)

Caton, D. B.; Gray, R. O.; Pollock, J. T.; Burns, W. C.; Hawkins, R. L.

1993-12-01

New lab exercises are presented, continuing a series developed over the past few years (see Caton and Pollock, BAAS 18 No. 4, 22 Nos. 1 and 4, and 23 No.4, and 24 No. 4), including a PC-based spectral classification exercise that displays spectra on a VGA screen, and a simple PC-based eclipsing binary light curve simulation program. Information packets will be available, including software, lab exercises, and instructor's notes. These will be on paper and on (PC) disk, in ASCII and WordPerfect formats, including some figures in AutoDesk's Generic CADD form. This will allow other institutions to modify the exercises to fit their own equipment and procedures. Information will also be provided on accessing a PC that we have installed on the Internet to allow acquisition of future updates and additional materials that will be developed.

Vortex shedding experiment with flat and curved bluff plates in water

NASA Technical Reports Server (NTRS)

Reed, D.; Nesman, T.; Howard, P.

1988-01-01

Vortex shedding experiments were conducted in a water flow facility in order to simulate the strong discrete 4000-Hz vibration detected in the Space Shuttle Main Engine (SSME) which is thought to be associated with the SSME LOX inlet tee splitter vanes on the Main Injector. For the case of a flat vane with a blunt trailing edge excited by flow induced vortex shedding, lock-in with the first bending mode of the plate was observed. A curved vane displayed similar behavior, with the lock-in being a more discrete higher amplitude response. Aluminum vanes were employed to decouple the first vane bending mode from the vortex shedding mode. The application of an asymmetric 30-deg trailing edge bevel to both the flat and curved vanes was found to greatly reduce the strength of the shed vortices.

Generalized Nanosatellite Avionics Testbed Lab

NASA Technical Reports Server (NTRS)

Frost, Chad R.; Sorgenfrei, Matthew C.; Nehrenz, Matt

2015-01-01

The Generalized Nanosatellite Avionics Testbed (G-NAT) lab at NASA Ames Research Center provides a flexible, easily accessible platform for developing hardware and software for advanced small spacecraft. A collaboration between the Mission Design Division and the Intelligent Systems Division, the objective of the lab is to provide testing data and general test protocols for advanced sensors, actuators, and processors for CubeSat-class spacecraft. By developing test schemes for advanced components outside of the standard mission lifecycle, the lab is able to help reduce the risk carried by advanced nanosatellite or CubeSat missions. Such missions are often allocated very little time for testing, and too often the test facilities must be custom-built for the needs of the mission at hand. The G-NAT lab helps to eliminate these problems by providing an existing suite of testbeds that combines easily accessible, commercial-offthe- shelf (COTS) processors with a collection of existing sensors and actuators.

The Gamma-Ray Burst ToolSHED is Open for Business

NASA Astrophysics Data System (ADS)

Giblin, Timothy W.; Hakkila, Jon; Haglin, David J.; Roiger, Richard J.

2004-09-01

The GRB ToolSHED, a Gamma-Ray Burst SHell for Expeditions in Data-Mining, is now online and available via a web browser to all in the scientific community. The ToolSHED is an online web utility that contains pre-processed burst attributes of the BATSE catalog and a suite of induction-based machine learning and statistical tools for classification and cluster analysis. Users create their own login account and study burst properties within user-defined multi-dimensional parameter spaces. Although new GRB attributes are periodically added to the database for user selection, the ToolSHED has a feature that allows users to upload their own burst attributes (e.g. spectral parameters, etc.) so that additional parameter spaces can be explored. A data visualization feature using GNUplot and web-based IDL has also been implemented to provide interactive plotting of user-selected session output. In an era in which GRB observations and attributes are becoming increasingly more complex, a utility such as the GRB ToolSHED may play an important role in deciphering GRB classes and understanding intrinsic burst properties.

Demonstrating the Effects of Light Quality on Plant Growth.

ERIC Educational Resources Information Center

Whitesell, J. H.; Garcia, Maria

1977-01-01

Describes a lab demonstration that illustrates the effect of different colors or wavelengths of visible light on plant growth and development. This demonstration is appropriate for use in college biology, botany, or plant physiology courses. (HM)

Comparison of three nonlinear models to describe long-term tag shedding by lake trout

USGS Publications Warehouse

Fabrizio, Mary C.; Swanson, Bruce L.; Schram, Stephen T.; Hoff, Michael H.

1996-01-01

We estimated long-term tag-shedding rates for lake trout Salvelinus namaycush using two existing models and a model we developed to account for the observed permanence of some tags. Because tag design changed over the course of the study, we examined tag-shedding rates for three types of numbered anchor tags (Floy tags FD-67, FD-67C, and FD-68BC) and an unprinted anchor tag (FD-67F). Lake trout from the Gull Island Shoal region, Lake Superior, were double-tagged, and subsequent recaptures were monitored in annual surveys conducted from 1974 to 1992. We modeled tag-shedding rates, using time at liberty and probabilities of tag shedding estimated from fish released in 1974 and 1978–1983 and later recaptured. Long-term shedding of numbered anchor tags in lake trout was best described by a nonlinear model with two parameters: an instantaneous tag-shedding rate and a constant representing the proportion of tags that were never shed. Although our estimates of annual shedding rates varied with tag type (0.300 for FD-67, 0.441 for FD-67C, and 0.656 for FD-68BC), differences were not significant. About 36% of tags remained permanently affixed to the fish. Of the numbered tags that were shed (about 64%), two mechanisms contributed to tag loss: disintegration and dislodgment. Tags from about 11% of recaptured fish had disintegrated, but most tags were dislodged. Unprinted tags were shed at a significant but low rate immediately after release, but the long-term, annual shedding rate of these tags was only 0.013. Compared with unprinted tags, numbered tags dislodged at higher annual rates; we hypothesized that this was due to the greater frictional drag associated with the larger cross-sectional area of numbered tags.

Teaching Diffraction of Light and Electrons: Classroom Analogies to Classic Experiments

ERIC Educational Resources Information Center

Velentzas, Athanasios

2014-01-01

Diffraction and interference are phenomena that demonstrate the wave nature of light and of particles. Experiments relating to the diffraction/interference of light can easily be carried out in an educational lab, but it may be impossible to perform experiments involving electrons because of the lack of specialized equipment needed for such…

Replication and shedding of MERS-CoV in Jamaican fruit bats (Artibeus jamaicensis)

PubMed Central

Munster, Vincent J.; Adney, Danielle R.; van Doremalen, Neeltje; Brown, Vienna R.; Miazgowicz, Kerri L.; Milne-Price, Shauna; Bushmaker, Trenton; Rosenke, Rebecca; Scott, Dana; Hawkinson, Ann; de Wit, Emmie; Schountz, Tony; Bowen, Richard A.

2016-01-01

The emergence of Middle East respiratory syndrome coronavirus (MERS-CoV) highlights the zoonotic potential of Betacoronaviruses. Investigations into the origin of MERS-CoV have focused on two potential reservoirs: bats and camels. Here, we investigated the role of bats as a potential reservoir for MERS-CoV. In vitro, the MERS-CoV spike glycoprotein interacted with Jamaican fruit bat (Artibeus jamaicensis) dipeptidyl peptidase 4 (DPP4) receptor and MERS-CoV replicated efficiently in Jamaican fruit bat cells, suggesting there is no restriction at the receptor or cellular level for MERS-CoV. To shed light on the intrinsic host-virus relationship, we inoculated 10 Jamaican fruit bats with MERS-CoV. Although all bats showed evidence of infection, none of the bats showed clinical signs of disease. Virus shedding was detected in the respiratory and intestinal tract for up to 9 days. MERS-CoV replicated transiently in the respiratory and, to a lesser extent, the intestinal tracts and internal organs; with limited histopathological changes observed only in the lungs. Analysis of the innate gene expression in the lungs showed a moderate, transient induction of expression. Our results indicate that MERS-CoV maintains the ability to replicate in bats without clinical signs of disease, supporting the general hypothesis of bats as ancestral reservoirs for MERS-CoV. PMID:26899616

Planning a Computer Lab: Considerations To Ensure Success.

ERIC Educational Resources Information Center

IALL Journal of Language Learning Technologies, 1994

1994-01-01

Presents points to consider when organizing a computer laboratory. These include the lab's overall objectives and how best to meet them; what type of students will use the lab; where the lab will be located; and what software and hardware can best meet the lab's overall objectives, population, and location requirements. Other factors include time,…

TQM in a Computer Lab.

ERIC Educational Resources Information Center

Swanson, Dewey A.; Phillips, Julie A.

At the Purdue University School of Technology (PST) at Columbus, Indiana, the Total Quality Management (TQM) philosophy was used in the computer laboratories to better meet student needs. A customer satisfaction survey was conducted to gather data on lab facilities, lab assistants, and hardware/software; other sections of the survey included…

Berkeley Lab - Materials Sciences Division

Science.gov Websites

Synthesis Condensed Matter and Materials Physics Scattering and Instrumentation Science Centers Center for Berkeley Lab Berkeley Lab A-Z Index Phone Book Jobs Search DOE Search MSD Go MSD - Materials Sciences Division About Organization Contact Research Core Programs Materials Discovery, Design and

Addressing Physics Grand Challenges Using the Jefferson Lab FEL

NASA Astrophysics Data System (ADS)

Williams, Gwyn P.

2006-11-01

The Jefferson Lab Free Electron Laser[1] is the first of the so-called 4^th generation light sources to go operational. Capable of delivering extraordinarily bright, tunable light in ultrafast pulses from THz[2] through infrared to UV, the facility extends the experimental reach of accelerator-based light-sources by many orders of magnitude. This allows new opportunities to study many of the ``Grand Challenges'' recently defined by the Office of Science, Basic Energy Sciences Division, most of which are concerned with understandings of equilibrium and non-equilibrium behavior of materials in physics, chemistry and biology using precise pump and probe techniques. Specifically, in condensed matter physics, the JLab FEL permits new studies which go beyond earlier studies of reductionist behavior to those which examine emergent behavior. Thus, the understanding of high Tc superconductivity, colossal magneto-resistance, and observations of the breakdown of the Born-Oppenheimer approximation, are examples of collective behavior which is now treated theoretically via the concept of quasiparticles. In this presentation we will describe the dual pathways of light source development and physics challenges, and then show how they are combined in experiments that allow new insights to be developed to understand material function. We will illustrate this with details of the evolution of accelerator-based light sources, and with examples of work performed to date. References: [1] Neil et al. Phys. Rev.Letts 84, 662 (2000). [2] Carr, Martin, McKinney, Neil, Jordan & Williams, Nature 420, 153 (2002).

Low flow vortex shedding flowmeter for hypergolics/all media

NASA Technical Reports Server (NTRS)

Thinh, Ngo

1990-01-01

A family of vortex shedding flowmeters for flow measurement of hypergols that requires a long term operation without removal from system lines was further developed. A family of vortex shedding flowmeters without moving parts was designed. The test loop to evaluate the meters for the Freon flow, which simulates the hypergolic fluids, was modified and reconstructed. Preliminary results were obtained on the output frequency characteristics of an 1/2 inch flowmeter as a function of the flow rate.

Men's re-placement: Social practices in a Men's Shed.

PubMed

Anstiss, David; Hodgetts, Darrin; Stolte, Ottilie

2018-05-06

Transitions into retirement can be difficult at the best of times. Many men find themselves having to reflect on who they are and what their lives are about. Their access to social supports and material resources are often disrupted. Men's Sheds offer a space where retired men can actively pursue wellbeing, and respond to disruption and loneliness through emplaced community practices. This paper draws on ethnographic research in a Men's Shed in Auckland, New Zealand in order to explore the social practices through which men create a shared space for themselves in which they can engage in meaningful relationships with each other. We document how participants work in concert to create a space in which they can be together through collective labour. Their emplacement in the shed affords opportunities for supported transitions into retirement and for engaging healthy lives beyond paid employment. Copyright © 2018 Elsevier Ltd. All rights reserved.

Life-long shedding of Puumala hantavirus in wild bank voles (Myodes glareolus).

PubMed

Voutilainen, Liina; Sironen, Tarja; Tonteri, Elina; Bäck, Anne Tuiskunen; Razzauti, Maria; Karlsson, Malin; Wahlström, Maria; Niemimaa, Jukka; Henttonen, Heikki; Lundkvist, Åke

2015-06-01

The knowledge of viral shedding patterns and viraemia in the reservoir host species is a key factor in assessing the human risk of zoonotic viruses. The shedding of hantaviruses (family Bunyaviridae) by their host rodents has widely been studied experimentally, but rarely in natural settings. Here we present the dynamics of Puumala hantavirus (PUUV) shedding and viraemia in naturally infected wild bank voles (Myodes glareolus). In a monthly capture-mark-recapture study, we analysed 18 bank voles for the presence and relative quantity of PUUV RNA in the excreta and blood from 2 months before up to 8 months after seroconversion. The proportion of animals shedding PUUV RNA in saliva, urine and faeces peaked during the first month after seroconversion, but continued throughout the study period with only a slight decline. The quantity of shed PUUV in reverse transcription quantitative PCR (RT-qPCR) positive excreta was constant over time. In blood, PUUV RNA was present for up to 7 months but both the probability of viraemia and the virus load declined with time. Our findings contradict the current view of a decline in virus shedding after the acute phase and a short viraemic period in hantavirus infection - an assumption widely adopted in current epidemiological models. We suggest the life-long shedding as a means of hantaviruses to survive over host population bottlenecks, and to disperse in fragmented habitats where local host and/or virus populations face temporary extinctions. Our results indicate that the kinetics of pathogens in wild hosts may differ considerably from those observed in laboratory settings. © 2015 The Authors.

EPICS Channel Access Server for LabVIEW

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zhukov, Alexander P.

It can be challenging to interface National Instruments LabVIEW (http://www.ni.com/labview/) with EPICS (http://www.aps.anl.gov/epics/). Such interface is required when an instrument control program was developed in LabVIEW but it also has to be part of global control system. This is frequently useful in big accelerator facilities. The Channel Access Server is written in LabVIEW, so it works on any hardware/software platform where LabVIEW is available. It provides full server functionality, so any EPICS client can communicate with it.

Future{at}Labs.Prosperity Game{trademark}

DOE Office of Scientific and Technical Information (OSTI.GOV)

Beck, D.F.; Boyack, K.W.; Berman, M.

Prosperity Games{trademark} are an outgrowth and adaptation of move/countermove and seminar War Games, Prosperity Games{trademark} are simulations that explore complex issues in a variety of areas including economics, politics, sociology, environment, education, and research. These issues can be examined from a variety of perspectives ranging from global, macroeconomic and geopolitical viewpoint down to the details of customer/supplier/market interactions specific industries. All Prosperity Games{trademark} are unique in that both the game format and the player contributions vary from game to game. This report documents the Future{at}Labs.Prosperity Game{trademark} conducted under the sponsorship of the Industry Advisory Boards of the national labs, themore » national labs, Lockheed Martin Corporation, and the University of California. Players were drawn from all stakeholders involved including government, industry, labs, and academia. The primary objectives of this game were to: (1) explore ways to optimize the role of the multidisciplinary labs in serving national missions and needs; (2) explore ways to increase collaboration and partnerships among government, laboratories, universities, and industry; and (3) create a network of partnership champions to promote findings and policy options. The deliberations and recommendations of these players provided valuable insights as to the views of this diverse group of decision makers concerning the future of the labs.« less

Shedding light on the subject: introduction to illumination engineering and design for multidiscipline engineering students

NASA Astrophysics Data System (ADS)

Ronen, Ram S.; Smith, R. Frank

1995-10-01

Educating engineers and architects in Illumination Engineering and related subjects has become a very important field and a very satisfying and rewarding one. Main reasons include the need to significantly conserve lighting energy and meet government regulations while supplying appropriate light levels and achieving aesthetical requirements. The proliferation of new lamps, luminaries and lighting controllers many of which are 'energy savers' also helps a trend to seek help from lighting engineers when designing new commercial and residential buildings. That trend is believed to continue and grow as benefits become attractive and new government conservation regulations take affect. To make things even better one notices that Engineering and Science students in most disciplines make excellent candidates for Illumination Engineers because of their background and teaching them can move ahead at a brisk pace and be a rewarding experience nevertheless. In the past two years, Cal Poly Pomona College of Engineering has been the beneficiary of a DOE/California grant. Its purpose was to precipitate and oversee light curricula in various California community colleges and also develop and launch an Illumination Engineering minor at Cal Poly University. Both objectives have successfully been met. Numerous community colleges throughout California developed and are offering a sequence of six lighting courses leading to a certificate; the first graduating class is now coming out of both Cypress and Consumnes Community Colleges. At Cal Poly University a four course/laboratory sequence leading to a minor in Illumination Engineering (ILE) is now offered to upper division students in the College of Engineering, College of Science and College of Architecture and Design. The ILE sequence will briefly be described. The first course, Introduction to Illumination Engineering and its laboratory are described in more detail alter. Various methods of instruction including lectures, self work

Design of inquiry-oriented science labs: impacts on students' attitudes

NASA Astrophysics Data System (ADS)

Baseya, J. M.; Francis, C. D.

2011-11-01

Background: Changes in lab style can lead to differences in learning. Two inquiry-oriented lab styles are guided inquiry (GI) and problem-based (PB). Students' attitudes towards lab are important to consider when choosing between GI and PB styles during curriculum design. Purpose: We examined the degree to which lab experiences are explained by a GI or a PB lab style vs. students' attitudes towards specific aspects of the experience, reflected by perceived excitement (exc), difficulty (dif), time efficiency (eff) and association between lab and lecture material (help). Sample: Approximately 1000 students attending first-semester, college biology lab for science majors at the University of Colorado at Boulder, USA, participated in the study. Design and method: In 2007, two labs were run as GI and one as PB. Formats were switched in 2008. Attitudes were assessed with a post-semester survey. Results: Only the four attitude variables (not lab style) had a strong relationship with overall lab rating which was most strongly related to exc, followed by dif and help/eff. Dif and eff had the greatest influence on attitudes for or against GI vs. PB labs, and help and exc had little influence on a GI vs. a PB lab. Also, when dif was low, students' attitudes were not significantly different between PB and GI labs, but when dif was high, students' significantly rated GI labs higher than PB labs. Conclusions: Students' attitudes towards lab are more dependent on specific aspects of the experience than on lab style. Changes in GI vs. PB lab styles primarily influence dif and eff rather than exc and help. Dif may be an important factor to consider when implementing a lab in the PB vs. the GI format. It might be good to go with a GI when dif is high and a PB when dif is low.

The value of Autoimmune Syndrome Induced by Adjuvant (ASIA) - Shedding light on orphan diseases in autoimmunity.

PubMed

Segal, Yahel; Dahan, Shani; Sharif, Kassem; Bragazzi, Nicola Luigi; Watad, Abdulla; Amital, Howard

2018-05-01

Autoimmune Syndrome Induced by Adjuvant (ASIA) is a definition aimed to describe the common etiological process at the root of five clinical entities sharing similar symptomatology: macrophagic myofasciitis syndrome (MMF), Gulf War Syndrome (GWS), sick building syndrome (SBS), siliconosis, and post vaccination autoimmune phenomena. ASIA illustrates the role of environmental immune stimulating agents, or adjuvants, in the instigation of complex autoimmune reactions among individuals bearing a genetic preponderance for autoimmunity. The value of ASIA lies first in the acknowledgment it provides for patients suffering from these as yet ill-defined medical conditions. Equally important is the spotlight it sheds for further research of these poorly understood conditions sharing a common pathogenesis. In this article we elaborate on the significance of ASIA, review the current evidence in support of the syndrome, and address recent reservations raised regarding its validity. Copyright © 2018 Elsevier B.V. All rights reserved.

Science Lab: A Peer Approach.

ERIC Educational Resources Information Center

Ronca, Courtney C.

The two goals of this program were to increase the number of classroom teachers using the lab and to increase the amount of time that the science lab was used. The solution strategy chosen was a combination of peer tutoring, orientation presentations, small group discovery experiments and activities, and individual science experiment stations. The…

A bankruptcy problem approach to load-shedding in multiagent-based microgrid operation.

PubMed

Kim, Hak-Man; Kinoshita, Tetsuo; Lim, Yujin; Kim, Tai-Hoon

2010-01-01

A microgrid is composed of distributed power generation systems (DGs), distributed energy storage devices (DSs), and loads. To maintain a specific frequency in the islanded mode as an important requirement, the control of DGs' output and charge action of DSs are used in supply surplus conditions and load-shedding and discharge action of DSs are used in supply shortage conditions. Recently, multiagent systems for autonomous microgrid operation have been studied. Especially, load-shedding, which is intentional reduction of electricity use, is a critical problem in islanded microgrid operation based on the multiagent system. Therefore, effective schemes for load-shedding are required. Meanwhile, the bankruptcy problem deals with dividing short resources among multiple agents. In order to solve the bankruptcy problem, division rules, such as the constrained equal awards rule (CEA), the constrained equal losses rule (CEL), and the random arrival rule (RA), have been used. In this paper, we approach load-shedding as a bankruptcy problem. We compare load-shedding results by above-mentioned rules in islanded microgrid operation based on wireless sensor network (WSN) as the communication link for an agent's interactions.

Estimating heritability of wool shedding in a cross-bred ewe population

USDA-ARS?s Scientific Manuscript database

Low wool prices and high production costs in sheep systems have resulted in the introduction of hair sheep genetics into flocks to reduce shearing costs. Wool shedding occurs naturally in a few breeds, and can be incorporated by crossbreeding. The opportunity to enhance shedding through selection de...

NASA's AVIRIS Instrument Sheds New Light on Southern California Wildfires

NASA Image and Video Library

2017-12-08

NASA's Airborne Visible Infrared Imaging Spectrometer instrument (AVIRIS), flying aboard a NASA Armstrong Flight Research Center high-altitude ER-2 aircraft, flew over the wildfires burning in Southern California on Dec. 5, 2017 and acquired this false-color image. Active fires are visible in red, ground surfaces are in green and smoke is in blue. AVIRIS is an imaging spectrometer that observes light in visible and infrared wavelengths, measuring the full spectrum of radiated energy. Unlike regular cameras with three colors, AVIRIS has 224 spectral channels from the visible through the shortwave infrared. This permits mapping of fire temperatures, fractional coverage, and surface properties, including how much fuel is available for a fire. Spectroscopy is also valuable for characterizing forest drought conditions and health to assess fire risk. AVIRIS has been observing fire-prone areas in Southern California for many years, forming a growing time series of before/after data cubes. These data are helping improve scientific understanding of fire risk and how ecosystems respond to drought and fire. https://photojournal.jpl.nasa.gov/catalog/PIA11243

3D-printed lab-on-valve for fluorescent determination of cadmium and lead in water.

PubMed

Mattio, Elodie; Robert-Peillard, Fabien; Vassalo, Laurent; Branger, Catherine; Margaillan, André; Brach-Papa, Christophe; Knoery, Joël; Boudenne, Jean-Luc; Coulomb, Bruno

2018-06-01

In recent years, the development of 3D printing in flow analysis has allowed the creation of new systems with various applications. Up to now, 3D printing was mainly used for the manufacture of small units such as flow detection cells, preconcentration units or mixing systems. In the present study, a new 3D printed lab-on-valve system was developed to selectively quantify lead and cadmium in water. Different technologies were compared for lab-on-valve 3D printing. Printed test units have shown that stereolithography or digital light processing are satisfactory techniques for creating complex lab-on-valve units. The lab-on-valve system was composed of two columns, eight peripheral ports and a central port, and a coil integrating baffles to increase mixing possibilities. A selective extraction of lead was first carried out by TrisKem Pb™ Resin column. Then, cadmium not retained on the first column was extracted on a second column of Amberlite® IR 120 resin. In a following step, lead and cadmium were eluted with ammonium oxalate and potassium iodide, respectively. Finally, the two metals were sequentially detected by the same Rhod-5N™ fluorescent reagent. This 3D printed lab-on-valve flow system allowed us to quantify lead and cadmium with a linear response from 0.2 to 15 µg L -1 and detection limits of 0.17 and 0.20 µg L -1 for lead and cadmium, respectively, which seems adapted for natural water analysis. Copyright © 2018 Elsevier B.V. All rights reserved.

Examining and contrasting the cognitive activities engaged in undergraduate research experiences and lab courses

NASA Astrophysics Data System (ADS)

Holmes, N. G.; Wieman, Carl E.

2016-12-01

While the positive outcomes of undergraduate research experiences (UREs) have been extensively categorized, the mechanisms for those outcomes are less understood. Through lightly structured focus group interviews, we have extracted the cognitive tasks that students identify as engaging in during their UREs. We also use their many comparative statements about their coursework, especially lab courses, to evaluate their experimental physics-related cognitive tasks in those environments. We find there are a number of cognitive tasks consistently encountered in physics UREs that are present in most experimental research. These are seldom encountered in lab or lecture courses, with some notable exceptions. Having time to reflect and fix or revise, and having a sense of autonomy, were both repeatedly cited as key enablers of the benefits of UREs. We also identify tasks encountered in actual experimental research that are not encountered in UREs. We use these findings to identify opportunities for better integration of the cognitive tasks in UREs and lab courses, as well as discussing the barriers that exist. This work responds to extensive calls for science education to better develop students' scientific skills and practices, as well as calls to expose more students to scientific research.

An Under-frequency Load Shedding Scheme with Continuous Load Control Proportional to Frequency Deviation

NASA Astrophysics Data System (ADS)

Li, Changgang; Sun, Yanli; Yu, Yawei

2017-05-01

Under frequency load shedding (UFLS) is an important measure to tackle with frequency drop caused by load-generation imbalance. In existing schemes, loads are shed by relays in a discontinuous way, which is the major reason leading to under-shedding and over-shedding problems. With the application of power electronics technology, some loads can be controlled continuously, and it is possible to improve the UFSL with continuous loads. This paper proposes an UFLS scheme by shedding loads continuously. The load shedding amount is proportional to frequency deviation before frequency reaches its minimum during transient process. The feasibility of the proposed scheme is analysed with analytical system frequency response model. The impacts of governor droop, system inertia, and frequency threshold on the performance of the proposed UFLS scheme are discussed. Cases are demonstrated to validate the proposed scheme by comparing it with conventional UFLS schemes.

Men's Sheds: enabling environments for Australian men living with and without long-term disabilities.

PubMed

Hansji, Neeraj L; Wilson, Nathan J; Cordier, Reinie

2015-05-01

The health of Australian men has recently received greater attention. Men's Sheds are named in national policy as an exemplar community-based organisation for the betterment of men's psychosocial health; yet, the evidence base to support this is limited. This study investigates the comparative experience of men with long-term disabilities and men without long-term disabilities who go to a Men's Shed and to what extent this provides these men with an enabling, as opposed to disabling, environment. Data were collected from 12 individual interviews with men with long-term disabilities (5) and men without long-term disabilities (6), including 1 interview with the male Men's Shed Coordinator (MSC); participant observation within the shed; and a document received from the female MSC regarding the funding the Shed receives. Interviews explored the men's experiences at the Shed and their sense of belonging and social inclusion. Participants had any type of long-term disability and had been attending the shed for a minimum of 1 month. Data were collected between May and September 2013 and were analysed using the constant comparative method of grounded theory. The core theme that emerged was an enabling community space. The four sub-themes were: a community and social hub; an equalising space; a safe and supportive male environment; and meaningful male activities. The current literature exemplifies Men's Sheds to be important community-based organisations beneficial to men's health and well-being. For men living with long-term disabilities, this study illuminates that Men's Sheds offer an environment of equality, facilitating a collegial and egalitarian culture. Men can partake in enabling activities and enjoy the company of other men enhancing their sense of belonging and social inclusion as well as interact with other community groups that occupy the same space as the Men's Shed. © 2014 John Wiley & Sons Ltd.

Looking south through west portion of C.W.E. Storage Shed (Bldg. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Looking south through west portion of C.W.E. Storage Shed (Bldg. 126) - Atchison, Topeka, Santa Fe Railroad, Albuquerque Shops, C.W.E. Storage Shed, 908 Second Street, Southwest, Albuquerque, Bernalillo County, NM

LANGUAGE LABS--AN UPDATED REPORT.

ERIC Educational Resources Information Center

1963

REPORTS FROM SEVERAL SCHOOL DISTRICTS ON THE USE OF AND PLANNING OF LANGUAGE LABORATORIES ARE PRESENTED. LABORATORIES SHOULD BE ARRANGED FOR FLEXIBLE USE. THE AVERAGE HIGH SCHOOL STUDENT CAN USE A LAB PROFITABLY FOR 20 TO 25 MINUTES. THERE ARE THREE DIFFERENT TYPES OF LANGUAGE LABORATORIES THAT ARE DESCRIBED. THE SATELLITE LAB IS DIVIDED BY A…

Academic Pipeline and Futures Lab

DTIC Science & Technology

2016-02-01

AFRL-RY-WP-TR-2015-0186 ACADEMIC PIPELINE AND FUTURES LAB Brian D. Rigling Wright State University FEBRUARY 2016...DD-MM-YY) 2. REPORT TYPE 3. DATES COVERED (From - To) February 2016 Final 12 June 2009 – 30 September 2015 4. TITLE AND SUBTITLE ACADEMIC ...6 3 WSU ACADEMIC PIPELINE AND LAYERED SENSING FUTURES LAB (prepared by K

Berkeley Lab - Materials Sciences Division

Science.gov Websites

Synthesis Condensed Matter and Materials Physics Scattering and Instrumentation Science Centers Center for materials and phenomena at multiple time and length scales. Through our core programs and research centers Berkeley Lab Berkeley Lab A-Z Index Phone Book Jobs Search DOE Search MSD Go MSD - Materials

Constitutive Endocytosis of VEGFR2 Protects the Receptor against Shedding*

PubMed Central

Basagiannis, Dimitris; Christoforidis, Savvas

2016-01-01

VEGFR2 plays a fundamental role in blood vessel formation and in life threatening diseases, such as cancer angiogenesis and cardiovascular disorders. Although inactive growth factor receptors are mainly localized at the plasma membrane, VEGFR2 undergoes constitutive endocytosis (in the absence of ligand) and recycling. Intriguingly, the significance of these futile transport cycles of VEGFR2 remains unclear. Here we found that, unexpectedly, the function of constitutive endocytosis of VEGFR2 is to protect the receptor against plasma membrane cleavage (shedding), thereby preserving the functional state of the receptor until the time of activation by VEGF. Inhibition of constitutive endocytosis of VEGFR2, by interference with the function of clathrin, dynamin, or Rab5, increases dramatically the cleavage/shedding of VEGFR2. Shedding of VEGFR2 produces an N-terminal soluble fragment (100 kDa, s100), which is released in the extracellular space, and a residual C-terminal part (130 kDa, p130) that remains integrated at the plasma membrane. The released soluble fragment (s100) co-immunoprecipitates with VEGF, in line with the topology of the VEGF-binding domain at the N terminus of VEGFR2. Increased shedding of VEGFR2 (via inhibition of constitutive endocytosis) results in reduced response to VEGF, consistently with the loss of the VEGF-binding domain from the membrane remnant of VEGFR2. These data suggest that constitutive internalization of VEGFR2 protects the receptor against shedding and provides evidence for an unprecedented mechanism via which endocytosis can regulate the fate and activity of growth factor receptors. PMID:27298320

Role of Hunk and Punc in Breast Cancer and Mammary Development

DTIC Science & Technology

2000-08-01

embryonic development of other organs, it is branes ( Santoro et al., 1996; Tamagnone and Comoglio, 1997; possible that EphA7 may play a lineage...Jacque- mammary carcinogenesis. Lab. Invest. 57, 112-137. mier, J., Jordan, B., Birnbaum, D., and Pebusque, M. J. (1999). Santoro , M. M., Collesi, C...involved may shed light on the broader physiological Centanni, J. M., de Miguel , M., Gopalan, G., Gilbert, D. J., Copeland, N. G., Jenkins, N. A

Effect of various visible light photoinitiators on the polymerization and color of light-activated resins.

PubMed

Arikawa, Hiroyuki; Takahashi, Hideo; Kanie, Takahito; Ban, Seiji

2009-07-01

The purpose of this study was to investigate effects of various visible light photoinitiators on the polymerization efficiency and color of the light-activated resins. Four photoinitiators, including camphorquinone, phenylpropanedione, monoacrylphosphine oxide (TPO), and bisacrylphosphine oxide (Ir819), were used. Each photoinitiator was dissolved in a Bis-GMA and TEGDMA monomer mixture. Materials were polymerized using dental quartz-tungsten halogen lamp (QTH), plasma-ark lamp and blue LED light-curing units, and a custom-made violet LED light unit. The degree of monomer conversion and CIE L*a*b* color values of the resins were measured using a FTIR and spectral transmittance meter. The degree of monomer conversions of TPO- and Ir819-containing resins polymerized with the violet-LED unit were higher than camphorquinone-containing resin polymerized with the QTH light-curing unit. The lowest color values were observed for the TPO-containing resin. Our results indicate that the TPO photoinitiator and the violet-LED light unit may provide a useful and improved photopolymerization system for dental light-activated resins.

Towards a Manifesto for Living Lab Co-creation

NASA Astrophysics Data System (ADS)

Følstad, Asbjørn; Brandtzæg, Petter Bae; Gulliksen, Jan; Börjeson, Mikael; Näkki, Pirjo

There is a growing interest in Living Labs for innovation and development in the field of information and communication technology. In particular there seem to be a tendency that current Living Labs aim to involve users for co-creative purposes. However, the current literature on Living Lab co-creation is severely limited. Therefore an Interact workshop is arranged as a first step towards a manifesto for Living Lab co-creation.

Berkeley Lab 2nd Grader Outreach

ScienceCinema

Scoggins, Jackie; Louie, Virginia

2017-12-11

The Berkeley Lab IT Department sponsored a community outreach program aimed at teaching young children about computers and networks. Second graders from LeConte Elementary School joined Lab IT Staff for a day of in-depth exercises and fun.

Labs: 1987.

ERIC Educational Resources Information Center

Igelsrud, Don, Ed.

1988-01-01

This article presents a variety of topics discussed in this column and at a biology teachers' workshop concerning the quality and value of lab techniques used for teaching high school biology. Topics included are Drosophila salivary glands, sea urchins, innovations, dyes and networking. (CW)

Berkeley Lab - Materials Sciences Division

Science.gov Websites

-486-6999 Urgent Radiation Protection Group Assistance Non-Life Threatening Event 24/7 Lab Phone: x7277 : 911 (no extentions required now) Non-Emergency Reporting (Fire and Police) Non-Life Threatening Event Spill Non-Life Threatening Event 24/7 Lab Phone: x6999 Cell Phone: 510-486-6999 Off Site Locations: 510

Report from the banding lab

USGS Publications Warehouse

Tautin, J.

1995-01-01

Mr. Tautin reported on the seemingly everchanging structure of biological science units within the Interior Department. Current Congressional proposals would either change the name of the Bird Banding Lab's parent agency or make it part of the Geological Survey. The current Congress has not looked favorably on science budgets within the Interior Department, and the Banding Lab's budget is being squeezed ever tighter.

78 FR 13213 - Regional Reliability Standard PRC-006-NPCC-1- Automatic Underfrequency Load Shedding

Federal Register 2010, 2011, 2012, 2013, 2014

2013-02-27

...; Order No. 775] Regional Reliability Standard PRC-006-NPCC-1--Automatic Underfrequency Load Shedding... transferred to the system upon loss of the facility.'' \\27\\ Compensatory load shedding is automatic shedding of load adequate to compensate for the loss of a generator due to the generator tripping early (i.e...

Community-based Men's Sheds: promoting male health, wellbeing and social inclusion in an international context.

PubMed

Cordier, Reinie; Wilson, Nathan J

2014-09-01

Males experience greater mortality and morbidity than females in most Western countries. The Australian and Irish National Male Health Policies aim to develop a framework to address this gendered health disparity. Men's Sheds have a distinct community development philosophy and are thus identified in both policies as an ideal location to address social isolation and positively impact the health and wellbeing of males who attend. The aim of this international cross-sectional survey was to gather information about Men's Sheds, the people who attend Men's Sheds, the activities at Men's Sheds, and the social and health dimensions of Men's Sheds. Results demonstrate that Men's Sheds are contributing a dual health and social role for a range of male subgroups. In particular, Men's Sheds have an outward social focus, supporting the social and mental health needs of men; health promotion and health literacy are key features of Men's Sheds. Men's Sheds have an important role to play in addressing the gendered health disparity that males face. They serve as an exemplar to health promotion professionals of a community development context where the aims of male health policy can be actualized as one part of a wider suite of global initiatives to reduce the gendered health disparity. © The Author (2013). Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

Persistent genital herpes simplex virus-2 shedding years following the first clinical episode.

PubMed

Phipps, Warren; Saracino, Misty; Magaret, Amalia; Selke, Stacy; Remington, Mike; Huang, Meei-Li; Warren, Terri; Casper, Corey; Corey, Lawrence; Wald, Anna

2011-01-15

Patients with newly acquired genital herpes simplex virus 2 (HSV-2) infection have virus frequently detected at the genital mucosa. Rates of genital shedding initially decrease over time after infection, but data on long-term viral shedding are lacking. For this study, 377 healthy adults with history of symptomatic genital HSV-2 infection collected anogenital swabs for HSV-2 DNA polymerase chain reaction for at least 30 consecutive days. Time since first genital herpes episode was significantly associated with reduced genital shedding. Total HSV shedding occurred on 33.6% of days in participants <1 year, 20.6% in those 1-9 years, and 16.7% in those ≥10 years from first episode. Subclinical HSV shedding occurred on 26.2% of days among participants <1 year, 13.1% in those 1-9 years, and 9.3% in those ≥10 years from first episode. On days with HSV detection, mean quantity was 4.9 log₁₀ copies/mL for those <1 year, 4.7 log₁₀ copies/mL among those 1-9 years, and 4.6 log₁₀ copies/mL among those ≥10 years since first episode. Rates of total and subclinical HSV-2 shedding decrease after the first year following the initial clinical episode. However, viral shedding persists at high rates and copy numbers years after infection, and therefore may pose continued risk of HSV-2 transmission to sexual partners.

Persistent Genital Herpes Simplex Virus-2 Shedding Years Following the First Clinical Episode

PubMed Central

Saracino, Misty; Magaret, Amalia; Selke, Stacy; Remington, Mike; Huang, Meei-Li; Casper, Corey; Corey, Lawrence; Wald, Anna

2011-01-01

Background. Patients with newly acquired genital herpes simplex virus 2 (HSV-2) infection have virus frequently detected at the genital mucosa. Rates of genital shedding initially decrease over time after infection, but data on long-term viral shedding are lacking. Methods. For this study, 377 healthy adults with history of symptomatic genital HSV-2 infection collected anogenital swabs for HSV-2 DNA polymerase chain reaction for at least 30 consecutive days. Results. Time since first genital herpes episode was significantly associated with reduced genital shedding. Total HSV shedding occurred on 33.6% of days in participants <1 year, 20.6% in those 1–9 years, and 16.7% in those ≥10 years from first episode. Subclinical HSV shedding occurred on 26.2% of days among participants <1 year, 13.1% in those 1–9 years, and 9.3% in those ≥10 years from first episode. On days with HSV detection, mean quantity was 4.9 log10 copies/mL for those <1 year, 4.7 log10 copies/mL among those 1–9 years, and 4.6 log10 copies/mL among those ≥10 years since first episode. Conclusions. Rates of total and subclinical HSV-2 shedding decrease after the first year following the initial clinical episode. However, viral shedding persists at high rates and copy numbers years after infection, and therefore may pose continued risk of HSV-2 transmission to sexual partners. PMID:21288817

Virus and host-specific differences in oral human herpesvirus shedding kinetics among Ugandan women and children.

PubMed

Matrajt, Laura; Gantt, Soren; Mayer, Bryan T; Krantz, Elizabeth M; Orem, Jackson; Wald, Anna; Corey, Lawrence; Schiffer, Joshua T; Casper, Corey

2017-10-12

Human herpesviruses (HHV) establish lifelong latent infection and are transmitted primarily via shedding at mucosal surfaces. Each HHV causes a unique spectrum of disease depending on the infected individual's age and immunity. We collected weekly oral swabs from young children and mothers in 32 Ugandan households for a median of one year. We characterized kinetics of oral shedding during primary and chronic infection for each virus. Cytomegalovirus (CMV), Epstein-Barr virus (EBV), and HHV-6 were shed at high rates following primary infection. The rate of oral herpes simplex virus (HSV) shedding was lower overall, and children and mothers with chronic HSV infection had lower shedding rates than children with primary infection. CMV shedding rate and viral load were higher in children with primary infection compared to children with chronic infection, and even lower in mothers with chronic infection. HHV-6 shedding rate and viral load were similar between children with primary or chronic infection, but lower in mothers. EBV shedding rate and quantity decreased less dramatically in mothers versus children, with HIV-positive mothers shedding at a higher rate than HIV-negative mothers. Each HHV has a distinct pattern of oral shedding which depends partially on the age and immune status of the host.

4. View north of Lake Whitney Dam. Wood shed at ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

4. View north of Lake Whitney Dam. Wood shed at center of photograph houses a turbine installed in 1932. Brick structure to the left of the turbine shed is a gate house which houses the main valves controlling flow of lake to water to the filter plant. - Lake Whitney Water Filtration Plant, Lake Whitney Dam, East side of Whitney Avenue near intersection with Armory Street, Hamden, New Haven County, CT

Computer-based Astronomy Labs for Non-science Majors

NASA Astrophysics Data System (ADS)

Smith, A. B. E.; Murray, S. D.; Ward, R. A.

1998-12-01

We describe and demonstrate two laboratory exercises, Kepler's Third Law and Stellar Structure, which are being developed for use in an astronomy laboratory class aimed at non-science majors. The labs run with Microsoft's Excel 98 (Macintosh) or Excel 97 (Windows). They can be run in a classroom setting or in an independent learning environment. The intent of the labs is twofold; first and foremost, students learn the subject matter through a series of informational frames. Next, students enhance their understanding by applying their knowledge in lab procedures, while also gaining familiarity with the use and power of a widely-used software package and scientific tool. No mathematical knowledge beyond basic algebra is required to complete the labs or to understand the computations in the spreadsheets, although the students are exposed to the concepts of numerical integration. The labs are contained in Excel workbook files. In the files are multiple spreadsheets, which contain either a frame with information on how to run the lab, material on the subject, or one or more procedures. Excel's VBA macro language is used to automate the labs. The macros are accessed through button interfaces positioned on the spreadsheets. This is done intentionally so that students can focus on learning the subject matter and the basic spreadsheet features without having to learn advanced Excel features all at once. Students open the file and progress through the informational frames to the procedures. After each procedure, student comments and data are automatically recorded in a preformatted Lab Report spreadsheet. Once all procedures have been completed, the student is prompted for a filename in which to save their Lab Report. The lab reports can then be printed or emailed to the instructor. The files will have full worksheet and workbook protection, and will have a "redo" feature at the end of the lab for students who want to repeat a procedure.

Sulforaphane inhibits endothelial protein C receptor shedding in vitro and in vivo.

PubMed

Ku, Sae-Kwang; Han, Min-Su; Bae, Jong-Sup

2014-10-01

Sulforaphane (SFN), a natural isothiocyanate present in cruciferous vegetables such as broccoli and cabbage, is effective in preventing carcinogenesis, diabetes, and inflammatory responses. Increasing evidence has demonstrated that beyond its role in the activation of protein C, endothelial cell protein C receptor (EPCR) is also involved in vascular inflammation. EPCR activity is markedly changed by ectodomain cleavage and its release as the soluble EPCR. EPCR can be shed from the cell surface, which is mediated by tumor necrosis factor-α converting enzyme (TACE). However, little is known about the effects of SFN on EPCR shedding. Our results demonstrated that SFN induced potent inhibition of phorbol-12-myristate 13-acetate (PMA)-, tumor necrosis factor (TNF)-α-, interleukin (IL)-1β, and cecal ligation and puncture (CLP)-induced EPCR shedding. SFN also inhibited the expression and activity of PMA-induced TACE in endothelial cells. In addition, treatment with SFN resulted in reduced PMA-stimulated phosphorylation of p38, extracellular regulated kinases (ERK) 1/2, and c-Jun N-terminal kinase (JNK). These results demonstrate the potential of SFN as an anti-sEPCR shedding reagent against PMA and CLP-mediated EPCR shedding. Copyright © 2014 Elsevier Inc. All rights reserved.

General relativistic considerations of the field shedding model of fast radio bursts

NASA Astrophysics Data System (ADS)

Punsly, Brian; Bini, Donato

2016-06-01

Popular models of fast radio bursts (FRBs) involve the gravitational collapse of neutron star progenitors to black holes. It has been proposed that the shedding of the strong neutron star magnetic field (B) during the collapse is the power source for the radio emission. Previously, these models have utilized the simplicity of the Schwarzschild metric which has the restriction that the magnetic flux is magnetic `hair' that must be shed before final collapse. But neutron stars have angular momentum and charge and a fully relativistic Kerr-Newman solution exists in which B has its source inside of the event horizon. In this Letter, we consider the magnetic flux to be shed as a consequence of the electric discharge of a metastable collapsed state of a Kerr-Newman black hole. It has also been argued that the shedding model will not operate due to pair creation. By considering the pulsar death line, we find that for a neutron star with B = 1011-1013 G and a long rotation period, >1s this is not a concern. We also discuss the observational evidence supporting the plausibility of magnetic flux shedding models of FRBs that are spawned from rapidly rotating progenitors.

A Bankruptcy Problem Approach to Load-shedding in Multiagent-based Microgrid Operation

PubMed Central

Kim, Hak-Man; Kinoshita, Tetsuo; Lim, Yujin; Kim, Tai-Hoon

2010-01-01

A microgrid is composed of distributed power generation systems (DGs), distributed energy storage devices (DSs), and loads. To maintain a specific frequency in the islanded mode as an important requirement, the control of DGs’ output and charge action of DSs are used in supply surplus conditions and load-shedding and discharge action of DSs are used in supply shortage conditions. Recently, multiagent systems for autonomous microgrid operation have been studied. Especially, load-shedding, which is intentional reduction of electricity use, is a critical problem in islanded microgrid operation based on the multiagent system. Therefore, effective schemes for load-shedding are required. Meanwhile, the bankruptcy problem deals with dividing short resources among multiple agents. In order to solve the bankruptcy problem, division rules, such as the constrained equal awards rule (CEA), the constrained equal losses rule (CEL), and the random arrival rule (RA), have been used. In this paper, we approach load-shedding as a bankruptcy problem. We compare load-shedding results by above-mentioned rules in islanded microgrid operation based on wireless sensor network (WSN) as the communication link for an agent’s interactions. PMID:22163386

Matrilysin (Matrix Metalloproteinase-7) Mediates E-Cadherin Ectodomain Shedding in Injured Lung Epithelium

PubMed Central

McGuire, John K.; Li, Qinglang; Parks, William C.

2003-01-01

Matrilysin (matrix metalloproteinase-7) is highly expressed in lungs of patients with pulmonary fibrosis and other conditions associated with airway and alveolar injury. Although matrilysin is required for closure of epithelial wounds ex vivo, the mechanism of its action in repair is unknown. We demonstrate that matrilysin mediates shedding of E-cadherin ectodomain from injured lung epithelium both in vitro and in vivo. In alveolar-like epithelial cells, transfection of activated matrilysin resulted in shedding of E-cadherin and accelerated cell migration. In vivo, matrilysin co-localized with E-cadherin at the basolateral surfaces of migrating tracheal epithelium, and the reorganization of cell-cell junctions seen in wild-type injured tissue was absent in matrilysin-null samples. E-cadherin ectodomain was shed into the bronchoalveolar lavage fluid of bleomycin-injured wild-type mice, but was not shed in matrilysin-null mice. These findings identify E-cadherin as a novel substrate for matrilysin and indicate that shedding of E-cadherin ectodomain is required for epithelial repair. PMID:12759241

Vortex shedding from obstacles: theoretical frequency prediction

NASA Astrophysics Data System (ADS)

Pier, Benoît

2001-11-01

The existence of self-sustained oscillations in spatially developing systems is closely related to the presence of a locally absolutely unstable region. A recent investigation of a ``synthetic wake'' (a wake with no solid obstacle and no reverse flow region) has proved [Pier and Huerre, J. Fluid Mech. 435, 145 (2001)] that the observed Kármán vortex street is a nonlinear elephant global mode. The same criterion is now shown to hold for real obstacles. Local properties are derived from the unperturbed basic flow computed by enforcing a symmetry condition on the central line. Application of the theoretical criterion then yields the expected Strouhal vortex shedding frequency. The thus predicted frequency is in excellent agreement with direct numerical simulations of the complete flow. The use of the frequency selection mechanism to control the vortex shedding will also be discussed.

Wake shed by an accelerating carangiform fish

NASA Astrophysics Data System (ADS)

Ting, Shang-Chieh; Yang, Jing-Tang

2008-11-01

We reveal an important fact that momentum change observed in the wake of an accelerating carangiform fish does not necessarily elucidate orientations of propulsive forces produced. An accelerating Crucian Carp (Carassius auratus) was found to shed a wake with net forward fluid momentum, which seemed drag-producing. Based on Newton's law, however, an accelerating fish is expected to shed a thrust wake with net rearward fluid momentum, rather than a drag wake. The unusual wake pattern observed is considered to be resulted primarily from the effect of pressure gradient created by accelerating movements of the fish. Ambient fluids tend to be sucked into low pressure zones behind an accelerating fish, resulting in forward orientations of jets recognizable in the wake. Accordingly, as to an accelerating fish, identifying force orientations from the wake requires considering also the effect of pressure gradient.

Enhanced absorption of light by charged nanoparticles.

PubMed

Rosenkrantz, Etai; Arnon, Shlomi

2010-04-15

We found that various charged nanoparticles (NPs) can raise the attenuation of electromagnetic (EM) radiation over 30 times more efficiently during resonance in comparison to equivalent noncharged particles for a given set of parameters. A condition that indicates a state of resonance between the incident EM radiation and the NP surface excitations is mathematically derived. Our results shed light on the mechanism responsible for the strong absorption of light by such charged NPs. The outcome of this research could help to design a new generation of communication devices as well as a new technique for biological cell imaging.

Constitutive Endocytosis of VEGFR2 Protects the Receptor against Shedding.

PubMed

Basagiannis, Dimitris; Christoforidis, Savvas

2016-08-05

VEGFR2 plays a fundamental role in blood vessel formation and in life threatening diseases, such as cancer angiogenesis and cardiovascular disorders. Although inactive growth factor receptors are mainly localized at the plasma membrane, VEGFR2 undergoes constitutive endocytosis (in the absence of ligand) and recycling. Intriguingly, the significance of these futile transport cycles of VEGFR2 remains unclear. Here we found that, unexpectedly, the function of constitutive endocytosis of VEGFR2 is to protect the receptor against plasma membrane cleavage (shedding), thereby preserving the functional state of the receptor until the time of activation by VEGF. Inhibition of constitutive endocytosis of VEGFR2, by interference with the function of clathrin, dynamin, or Rab5, increases dramatically the cleavage/shedding of VEGFR2. Shedding of VEGFR2 produces an N-terminal soluble fragment (100 kDa, s100), which is released in the extracellular space, and a residual C-terminal part (130 kDa, p130) that remains integrated at the plasma membrane. The released soluble fragment (s100) co-immunoprecipitates with VEGF, in line with the topology of the VEGF-binding domain at the N terminus of VEGFR2. Increased shedding of VEGFR2 (via inhibition of constitutive endocytosis) results in reduced response to VEGF, consistently with the loss of the VEGF-binding domain from the membrane remnant of VEGFR2. These data suggest that constitutive internalization of VEGFR2 protects the receptor against shedding and provides evidence for an unprecedented mechanism via which endocytosis can regulate the fate and activity of growth factor receptors. © 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

Zika Virus Shedding in Semen of Symptomatic Infected Men.

PubMed

Mead, Paul S; Duggal, Nisha K; Hook, Sarah A; Delorey, Mark; Fischer, Marc; Olzenak McGuire, Dana; Becksted, Heidi; Max, Ryan J; Anishchenko, Michael; Schwartz, Amy M; Tzeng, Wen-Pin; Nelson, Christina A; McDonald, Erin M; Brooks, John T; Brault, Aaron C; Hinckley, Alison F

2018-04-12

Zika virus (ZIKV) is an emerging mosquito-borne flavivirus that has been linked to adverse birth outcomes. Previous reports have shown that person-to-person transmission can occur by means of sexual contact. We conducted a prospective study involving men with symptomatic ZIKV infection to determine the frequency and duration of ZIKV shedding in semen and urine and to identify risk factors for prolonged shedding in these fluids. Specimens were obtained twice per month for 6 months after illness onset and were tested by real-time reverse-transcriptase-polymerase-chain-reaction (RT-PCR) assay for ZIKV RNA and by Vero cell culture and plaque assay for infectious ZIKV. A total of 1327 semen samples from 184 men and 1038 urine samples from 183 men were obtained 14 to 304 days after illness onset. ZIKV RNA was detected in the urine of 7 men (4%) and in the semen of 60 (33%), including in semen samples from 22 of 36 men (61%) who were tested within 30 days after illness onset. ZIKV RNA shedding in semen decreased substantially during the 3 months after illness onset but continued for 281 days in 1 man (1%). Factors that were independently associated with prolonged RNA shedding included older age, less frequent ejaculation, and the presence of certain symptoms at the time of initial illness. Infectious ZIKV was isolated from 3 of 78 semen samples with detectable ZIKV RNA, all obtained within 30 days after illness onset and all with at least 7.0 log 10 ZIKV RNA copies per milliliter of semen. ZIKV RNA was commonly present in the semen of men with symptomatic ZIKV infection and persisted in some men for more than 6 months. In contrast, shedding of infectious ZIKV appeared to be much less common and was limited to the first few weeks after illness onset. (Funded by the Centers for Disease Control and Prevention.).

Genetic factors controlling wool shedding in a composite Easycare sheep flock.

PubMed

Matika, O; Bishop, S C; Pong-Wong, R; Riggio, V; Headon, D J

2013-12-01

Historically, sheep have been selectively bred for desirable traits including wool characteristics. However, recent moves towards extensive farming and reduced farm labour have seen a renewed interest in Easycare breeds. The aim of this study was to quantify the underlying genetic architecture of wool shedding in an Easycare flock. Wool shedding scores were collected from 565 pedigreed commercial Easycare sheep from 2002 to 2010. The wool scoring system was based on a 10-point (0-9) scale, with score 0 for animals retaining full fleece and 9 for those completely shedding. DNA was sampled from 200 animals of which 48 with extreme phenotypes were genotyped using a 50-k SNP chip. Three genetic analyses were performed: heritability analysis, complex segregation analysis to test for a major gene hypothesis and a genome-wide association study to map regions in the genome affecting the trait. Phenotypes were treated as a continuous or binary variable and categories. High estimates of heritability (0.80 when treated as a continuous, 0.65-0.75 as binary and 0.75 as categories) for shedding were obtained from linear mixed model analyses. Complex segregation analysis gave similar estimates (0.80 ± 0.06) to those above with additional evidence for a major gene with dominance effects. Mixed model association analyses identified four significant (P < 0.05) SNPs. Further analyses of these four SNPs in all 200 animals revealed that one of the SNPs displayed dominance effects similar to those obtained from the complex segregation analyses. In summary, we found strong genetic control for wool shedding, demonstrated the possibility of a single putative dominant gene controlling this trait and identified four SNPs that may be in partial linkage disequilibrium with gene(s) controlling shedding. © 2013 University of Edinburgh, Animal Genetics © 2013 Stichting International Foundation for Animal Genetics.

Liquid Crystal Droplet-Based Amplification of Microvesicles that are Shed by Mammalian Cells

PubMed Central

Tan, Lie Na; Wiepz, Gregory J.; Miller, Daniel S.; Shusta, Eric V.; Abbott, Nicholas L.

2014-01-01

Membrane-derived microvesicles (MVs) shed by cells are being investigated for their role in intercellular communication and as potential biomarkers of disease, but facile and sensitive methods for their analysis do not exist. Here we demonstrate new principles for analysis of MVs that use micrometer-sized droplets of liquid crystals (LCs) to amplify MVs that are selectively captured via antibody-mediated interactions. The influence of the MVs on the micrometer-sized LC droplets is shown to be readily quantified via use of flow cytometry. The methodology was developed using MVs shed by epidermoid carcinoma A431 cells that contain epidermal growth factor receptor (EGFR) as an important and representative example of MVs containing signaling proteins that play a central role in cancer. The LC droplets were found to be sensitive to 106 MVs containing EGFR (relative to controls using isotype control antibody) and to possess a dynamic range of response across several orders of magnitude. Because the 100 nm-sized MVs captured via EGFR generate an optical response in the micrometer-sized LC droplets that can be readily detected by flow cytometry in light scattering mode, the approach possesses significant advantages over direct detection of MVs by flow cytometry. The LC droplets are also substantially more sensitive than techniques such as immunoblotting because the lipid-component of the MVs serves to amplify the antibody-mediated capture of the target proteins in the MVs. Other merits of the approach are defined and discussed in the paper. PMID:24667742

RICH Detector for Jefferson Labs CLAS12

NASA Astrophysics Data System (ADS)

Trotta, Richard; Torisky, Ben; Benmokhtar, Fatiha

2015-10-01

Jefferson Lab (Jlab) is performing a large-scale upgrade to its Continuous Electron Beam Accelerator Facility (CEBAF) up to 12GeV beams. The Large Acceptance Spectrometer (CLAS12) in Hall B is being upgraded and a new hybrid Ring Imaging Cherenkov (RICH) detector is being developed to provide better kaon - pion separation throughout the 3 to 8 GeV/c momentum range. This detector will be used for a variety of Semi-Inclusive Deep Inelastic Scattering experiments. Cherenkov light can be accurately detected by a large array of sophisticated Multi-Anode Photomultiplier Tubes (MA-PMT) and heavier particles, like kaons, will span the inner radii. We are presenting our work on the creation of the RICH's geometry within the CLAS12 java framework. This development is crucial for future calibration, reconstructions and analysis of the detector.

Evaluation of oral microbiology lab curriculum reform.

PubMed

Nie, Min; Gao, Zhen Y; Wu, Xin Y; Jiang, Chen X; Du, Jia H

2015-12-07

According to the updated concept of oral microbiology, the School of Stomatology, Wuhan University, has carried out oral microbiology teaching reforms during the last 5 years. There was no lab curriculum before 2009 except for a theory course of oral microbiology. The school has implemented an innovative curriculum with oral medicine characteristics to strengthen understanding of knowledge, cultivate students' scientific interest and develop their potential, to cultivate the comprehensive ability of students. This study was designed to evaluate the oral microbiology lab curriculum by analyzing student performance and perceptions regarding the curriculum from 2009 to 2013. The lab curriculum adopted modalities for cooperative learning. Students collected dental plaque from each other and isolated the cariogenic bacteria with selective medium plates. Then they purified the enrichment culture medium and identified the cariogenic strains by Gram stain and biochemical tests. Both quantitative and qualitative data for 5 years were analysed in this study. Part One of the current study assessed student performance in the lab from 2009 to 2013. Part Two used qualitative means to assess students' perceptions by an open questionnaire. The 271 study students' grades on oral microbiology improved during the lab curriculum: "A" grades rose from 60.5 to 81.2 %, and "C" grades fell from 28.4 to 6.3 %. All students considered the lab curriculum to be interesting and helpful. Quantitative and qualitative data converge to suggest that the lab curriculum has strengthened students' grasp of important microbiology-related theory, cultivated their scientific interest, and developed their potential and comprehensive abilities. Our student performance and perception data support the continued use of the innovative teaching system. As an extension and complement of the theory course, the oral microbiology lab curriculum appears to improve the quality of oral medicine education and help to

Long-term Shedding of Influenza A Virus in Stool of Immunocompromised Child

PubMed Central

Mix, Samantha; Rowe, Judy; Ikemoto, Sheryl; Baron, Ellen J.

2010-01-01

In immunocompromised patients, influenza infection may progress to prolonged viral shedding from the respiratory tract despite antiviral therapy. We describe chronic influenza A virus infection in an immunocompromised child who had prolonged shedding of culturable influenza virus in stool. PMID:20587197

8. MACHINERY SHED STORAGE ROOM ADDITION DETAIL SHOWING MATRIX OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

8. MACHINERY SHED STORAGE ROOM ADDITION DETAIL SHOWING MATRIX OF NAILS USED TO ADHERE PORTLAND CEMENT PLASTER, SOUTH ADOBE WALL ADJACENT TO WINDOW Note: Photographs Nos. AZ-159-A-9 through AZ-159-A-10 are photocopies of photographs. The original prints and negatives are located in the SCS Tucson Plant Materials Center, Tucson, Arizona. Photographer Ted F. Spaller. - Tucson Plant Material Center, Machinery Shed, 3241 North Romero Road, Tucson, Pima County, AZ

Innovation - A view from the Lab

USDA-ARS?s Scientific Manuscript database

The USDA Ag Lab in Peoria helps bridge the gap between agricultural producers and commercial manufacturers. In 2015, the Ag Lab, officially known as the Agricultural Research Service (ARS) National Center for Agricultural Utilization Research (NCAUR), is celebrating 75 years of research in Peoria. T...

LABS Foundational Technology

DOE Office of Scientific and Technical Information (OSTI.GOV)

Olson, Jerry

2012-01-01

They are the inventors of our generation dedicated to exceptional science, advancing the technologies of tomorrow. CO-LABS honors the outstanding achievements of researchers and their impact on the world.

View of McKenzieRichey barn showing shed design, rolled roofing and ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

View of McKenzie-Richey barn showing shed design, rolled roofing and wood shed roof, facing southwest - McKenzie Property, Barn, North Bank of Sailor Gulch, 750 feet northwest of intersection of U.S.F.S. Roads 651 & 349, Placerville, Boise County, ID

Managing Saginaw Bay nutrient loading by surrounding watersheds through near real time hydrologic resource sheds

EPA Science Inventory

We can quantify source areas contributing material to a location during various time periods as resource sheds. Various kinds of resource sheds and their source material distributions are defined. For watershed hydrology, we compute resource sheds and their source material distri...

Light composite scalar in twelve-flavor QCD on the lattice.

PubMed

Aoki, Yasumichi; Aoyama, Tatsumi; Kurachi, Masafumi; Maskawa, Toshihide; Nagai, Kei-ichi; Ohki, Hiroshi; Rinaldi, Enrico; Shibata, Akihiro; Yamawaki, Koichi; Yamazaki, Takeshi

2013-10-18

On the basis of lattice simulations using highly improved staggered quarks for twelve-flavor QCD with several bare fermion masses, we observe a flavor-singlet scalar state lighter than the pion in the correlators of fermionic interpolating operators. The same state is also investigated using correlators of gluonic interpolating operators. Combined with our previous study that showed twelve-flavor QCD to be consistent with being in the conformal window, we infer that the lightness of the scalar state is due to infrared conformality. This result shed some light on the possibility of a light composite Higgs boson ("technidilaton") in walking technicolor theories.

Birds Shed RNA-Viruses According to the Pareto Principle

PubMed Central

Jankowski, Mark D.; Williams, Christopher J.; Fair, Jeanne M.; Owen, Jennifer C.

2013-01-01

A major challenge in disease ecology is to understand the role of individual variation of infection load on disease transmission dynamics and how this influences the evolution of resistance or tolerance mechanisms. Such information will improve our capacity to understand, predict, and mitigate pathogen-associated disease in all organisms. In many host-pathogen systems, particularly macroparasites and sexually transmitted diseases, it has been found that approximately 20% of the population is responsible for approximately 80% of the transmission events. Although host contact rates can account for some of this pattern, pathogen transmission dynamics also depend upon host infectiousness, an area that has received relatively little attention. Therefore, we conducted a meta-analysis of pathogen shedding rates of 24 host (avian) – pathogen (RNA-virus) studies, including 17 bird species and five important zoonotic viruses. We determined that viral count data followed the Weibull distribution, the mean Gini coefficient (an index of inequality) was 0.687 (0.036 SEM), and that 22.0% (0.90 SEM) of the birds shed 80% of the virus across all studies, suggesting an adherence of viral shedding counts to the Pareto Principle. The relative position of a bird in a distribution of viral counts was affected by factors extrinsic to the host, such as exposure to corticosterone and to a lesser extent reduced food availability, but not to intrinsic host factors including age, sex, and migratory status. These data provide a quantitative view of heterogeneous virus shedding in birds that may be used to better parameterize epidemiological models and understand transmission dynamics. PMID:23991129

Berkeley Lab - Materials Sciences Division

Science.gov Websites

Berkeley Lab Berkeley Lab A-Z Index Phone Book Jobs Search DOE Search MSD Go MSD - Materials Investigators Ager, Joel W » Alivisatos, A Paul » Altman, Ehud » Analytis, James » Anderson, Christopher  , Naomi » Gullikson, Eric M » Harris, Stephen J » Hasan, M. Zahid » Hellman, Frances » Helms, Brett A

Experiential Learning of Digital Communication Using LabVIEW

ERIC Educational Resources Information Center

Zhan, Wei; Porter, Jay R.; Morgan, Joseph A.

2014-01-01

This paper discusses the design and implementation of laboratories and course projects using LabVIEW in an instrumentation course. The pedagogical challenge is to enhance students' learning of digital communication using LabVIEW. LabVIEW was extensively used in the laboratory sessions, which better prepared students for the course projects. Two…

Detail of C.W.E. Storage Shed (Bldg. 126) monorails and safety ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Detail of C.W.E. Storage Shed (Bldg. 126) monorails and safety sign for track workers. Machine Shop (Bldg. 134) is in the background - Atchison, Topeka, Santa Fe Railroad, Albuquerque Shops, C.W.E. Storage Shed, 908 Second Street, Southwest, Albuquerque, Bernalillo County, NM

Active Learning Strategies for Introductory Light and Optics

ERIC Educational Resources Information Center

Sokoloff, David R.

2016-01-01

There is considerable evidence that traditional approaches are ineffective in teaching physics concepts, including light and optics concepts. A major focus of the work of the Activity Based Physics Group has been on the development of active learning curricula like RealTime Physics (RTP) labs and Interactive Lecture Demonstrations (ILDs). Among…

Practical Physics Labs: A Resource Manual.

ERIC Educational Resources Information Center

Goodwin, Peter

This resource manual focuses on physics labs that relate to the world around us and utilize simple equipment and situations. Forty-five laboratories are included that relate to thermodynamics, electricity, magnetism, dynamics, optics, wave transmission, centripetal force, and atomic physics. Each lab has three sections. The first section…

My Brother’s Keeper National Lab Week

NASA Image and Video Library

2016-03-02

Students in the My Brother’s Keeper program line the railings of an observation deck overlooking the Granular Mechanics and Regolith Operations Lab at NASA’s Kennedy Space Center in Florida. The spaceport is one of six NASA centers that participated in My Brother’s Keeper National Lab Week. The event is a nationwide effort to bring youth from underrepresented communities into federal labs and centers for hands-on activities, tours and inspirational speakers. Sixty students from the nearby cities of Orlando and Sanford visited Kennedy, where they toured the Vehicle Assembly Building, the Space Station Processing Facility and the center’s innovative Swamp Works Labs. The students also had a chance to meet and ask questions of a panel of subject matter experts from across Kennedy.

My Brother’s Keeper National Lab Week

NASA Image and Video Library

2016-03-02

Students in the My Brother’s Keeper program try out some of the machinery inside the Prototype Lab at NASA’s Kennedy Space Center. The Florida spaceport is one of six NASA centers that participated in My Brother’s Keeper National Lab Week. The event is a nationwide effort to bring youth from underrepresented communities into federal labs and centers for hands-on activities, tours and inspirational speakers. Sixty students from the nearby cities of Orlando and Sanford visited Kennedy, where they toured the Vehicle Assembly Building, the Space Station Processing Facility and the center’s innovative Swamp Works Labs. The students also had a chance to meet and ask questions of a panel of subject matter experts from across Kennedy.

My Brother’s Keeper National Lab Week

NASA Image and Video Library

2016-03-02

Mike Lane demonstrates a 3D scanner inside the NASA Kennedy Space Center Prototype Lab for students in the My Brother’s Keeper program. The Florida spaceport is one of six NASA centers that participated in My Brother’s Keeper National Lab Week. The event is a nationwide effort to bring youth from underrepresented communities into federal labs and centers for hands-on activities, tours and inspirational speakers. Sixty students from the nearby cities of Orlando and Sanford visited Kennedy, where they toured the Vehicle Assembly Building, the Space Station Processing Facility and the center’s innovative Swamp Works Labs. The students also had a chance to meet and ask questions of a panel of subject matter experts from across Kennedy.

My Brother’s Keeper National Lab Week

NASA Image and Video Library

2016-03-02

Harold (Russ) McAmis demonstrates machinery inside NASA Kennedy Space Center’s Prototype Lab for students in the My Brother’s Keeper program. The Florida spaceport is one of six NASA centers that participated in My Brother’s Keeper National Lab Week. The event is a nationwide effort to bring youth from underrepresented communities into federal labs and centers for hands-on activities, tours and inspirational speakers. Sixty students from the nearby cities of Orlando and Sanford visited Kennedy, where they toured the Vehicle Assembly Building, the Space Station Processing Facility and the center’s innovative Swamp Works Labs. The students also had a chance to meet and ask questions of a panel of subject matter experts from across Kennedy.

My Brother’s Keeper National Lab Week

NASA Image and Video Library

2016-03-02

Jose Nunez of NASA Kennedy Space Center’s Exploration Research and Technology Programs talks to students in the My Brother’s Keeper program outside the Florida spaceport’s Swamp Works Lab. Kennedy is one of six NASA centers that participated in My Brother’s Keeper National Lab Week. The event is a nationwide effort to bring youth from underrepresented communities into federal labs and centers for hands-on activities, tours and inspirational speakers. Sixty students from the nearby cities of Orlando and Sanford visited Kennedy, where they toured the Vehicle Assembly Building, the Space Station Processing Facility and the center’s innovative Swamp Works Labs. The students also had a chance to meet and ask questions of a panel of subject matter experts from across Kennedy.

Shedding of dual structures in the wake of a surface-mounted low aspect ratio cone

NASA Astrophysics Data System (ADS)

Chen, Zixiang; Martinuzzi, Robert J.

2018-04-01

The periodic shedding of vortex pairs in the turbulent wake of a surface-mounted right cone of aspect ratio 0.867 protruding a thin turbulent boundary layer is investigated experimentally. A phase-averaged volumetric velocity field is reconstructed from planar stereoscopic particle image velocimetry. During a typical (phase-averaged) shedding cycle, counter-rotating base vortices alternately form. These are tilted and stretched to merge with stream-wise tip vortices. The merged structure sheds and is convected downstream. A synthesis of earlier observations suggests that a similar shedding process exists for other low aspect ratio tapered geometries and is more complex than the shedding patterns observed for cantilevered cylinders, despite similarities of the mean flow field structure.

Baseball Physics: A New Mechanics Lab

NASA Astrophysics Data System (ADS)

Wagoner, Kasey; Flanagan, Daniel

2018-05-01

The game of baseball provides an interesting laboratory for experimenting with mechanical phenomena (there are many good examples in The Physics Teacher, available on Professor Alan Nathan's website, and discussed in Physics of Baseball & Softball). We have developed a lab, for an introductory-level physics course, that investigates many of these phenomena. The lab uses inexpensive, readily available equipment such as wooden baseball bats, baseballs, and actual Major League Baseball data. By the end of the lab, students have revisited many concepts they learned earlier in the semester and come away with an understanding of how to put seemingly disparate ideas together to analyze a fun sport.

Teaching Chemistry Lab Safety through Comics

NASA Astrophysics Data System (ADS)

di Raddo, Pasquale

2006-04-01

As a means for raising students' interest in aspects pertaining to chemistry lab safety, this article presents a novel approach to teaching this important subject. Comic book lab scenes that involve fictional characters familiar to many students are presented and discussed as to the safety concerns represented in those images. These are discussed in a safety prelab session. For the sake of comparison, students are then shown images taken from current chemistry journals of safety-conscious contemporary chemists at work in their labs. Finally the need to adhere to copyright regulations for the use of the images is discussed so as to increase students' awareness of academic honesty and copyright issues.

Secretome analysis to elucidate metalloprotease-dependent ectodomain shedding of glycoproteins during neuronal differentiation.

PubMed

Tsumagari, Kazuya; Shirakabe, Kyoko; Ogura, Mayu; Sato, Fuminori; Ishihama, Yasushi; Sehara-Fujisawa, Atsuko

2017-02-01

Many membrane proteins are subjected to limited proteolyses at their juxtamembrane regions, processes referred to as ectodomain shedding. Shedding ectodomains of membrane-bound ligands results in activation of downstream signaling pathways, whereas shedding those of cell adhesion molecules causes loss of cell-cell contacts. Secreted proteomics (secretomics) using high-resolution mass spectrometry would be strong tools for both comprehensive identification and quantitative measurement of membrane proteins that undergo ectodomain shedding. In this study, to elucidate the ectodomain shedding events that occur during neuronal differentiation, we establish a strategy for quantitative secretomics of glycoproteins released from differentiating neuroblastoma cells into culture medium with or without GM6001, a broad-spectrum metalloprotease inhibitor. Considering that most of transmembrane and secreted proteins are N-glycosylated, we include a process of N-glycosylated peptides enrichment as well as isotope tagging in our secretomics workflow. Our results show that differentiating N1E-115 neurons secrete numerous glycosylated polypeptides in metalloprotease-dependent manners. They are derived from cell adhesion molecules such as NCAM1, CADM1, L1CAM, various transporters and receptor proteins. These results show the landscape of ectodomain shedding and other secretory events in differentiating neurons and/or during axon elongation, which should help elucidate the mechanism of neurogenesis and the pathogenesis of neurological disorders. © 2017 Molecular Biology Society of Japan and John Wiley & Sons Australia, Ltd.

eduSPIM: Light Sheet Microscopy in the Museum

PubMed Central

Schmid, Benjamin; Weber, Michael; Huisken, Jan

2016-01-01

Light Sheet Microscopy in the Museum Light sheet microscopy (or selective plane illumination microscopy) is an important imaging technique in the life sciences. At the same time, this technique is also ideally suited for community outreach projects, because it produces visually appealing, highly dynamic images of living organisms and its working principle can be understood with basic optics knowledge. Still, the underlying concepts are widely unknown to the non-scientific public. On the occasion of the UNESCO International Year of Light, a technical museum in Dresden, Germany, launched a special, interactive exhibition. We built a fully functional, educational selective plane illumination microscope (eduSPIM) to demonstrate how developments in microscopy promote discoveries in biology. Design Principles of an Educational Light Sheet Microscope To maximize educational impact, we radically reduced a standard light sheet microscope to its essential components without compromising functionality and incorporated stringent safety concepts beyond those needed in the lab. Our eduSPIM system features one illumination and one detection path and a sealed sample chamber. We image fixed zebrafish embryos with fluorescent vasculature, because the structure is meaningful to laymen and visualises the optical principles of light sheet microscopy. Via a simplified interface, visitors acquire fluorescence and transmission data simultaneously. The eduSPIM Design Is Tailored Easily to Fit Numerous Applications The universal concepts presented here may also apply to other scientific approaches that are communicated to laymen in interactive settings. The specific eduSPIM design is adapted easily for various outreach and teaching activities. eduSPIM may even prove useful for labs needing a simple SPIM. A detailed parts list and schematics to rebuild eduSPIM are provided. PMID:27560188

Particle shedding from peristaltic pump tubing in biopharmaceutical drug product manufacturing.

PubMed

Saller, Verena; Matilainen, Julia; Grauschopf, Ulla; Bechtold-Peters, Karoline; Mahler, Hanns-Christian; Friess, Wolfgang

2015-04-01

In a typical manufacturing setup for biopharmaceutical drug products, the fill and dosing pump is placed after the final sterile filtration unit in order to ensure adequate dispensing accuracy and avoid backpressure peaks. Given the sensitivity of protein molecules, peristaltic pumps are often preferred over piston pumps. However, particles may be shed from the silicone tubing employed. In this study, particle shedding and a potential turbidity increase during peristaltic pumping of water and buffer were investigated using three types of commercially available silicone tubing. In the recirculates, mainly particles of around 200 nm next to a very small fraction of particles in the lower micrometer range were found. Using 3D laser scanning microscopy, surface roughness of the inner tubing surface was found to be a determining factor for particle shedding from silicone tubing. As the propensity toward particle shedding varied between tubing types and also cannot be concluded from manufacturer's specifications, individual testing with the presented methods is recommended during tubing qualification. Choosing low abrasive tubing can help to further minimize the very low particle counts to be expected in pharmaceutical drug products. © 2015 Wiley Periodicals, Inc. and the American Pharmacists Association.

A Study on a Centralized Under-Voltage Load Shedding Scheme Considering the Load Characteristics

NASA Astrophysics Data System (ADS)

Deng, Jiyu; Liu, Junyong

Under-voltage load shedding is an important measure for maintaining voltage stability.Aiming at the optimal load shedding problem considering the load characteristics,firstly,the traditional under-voltage load shedding scheme based on a static load model may cause the analysis inaccurate is pointed out on the equivalent Thevenin circuit.Then,the dynamic voltage stability margin indicator is derived through local measurement.The derived indicator can reflect the voltage change of the key area in a myopia linear way.Dimensions of the optimal problem will be greatly simplified using this indicator.In the end,mathematical model of the centralized load shedding scheme is built with the indicator considering load characteristics.HSPPSO is introduced to slove the optimal problem.Simulation results on IEEE-39 system show that the proposed scheme display a good adaptability in solving the under-voltage load shedding considering dynamic load characteristics.

Hydrogel Beads: The New Slime Lab?

ERIC Educational Resources Information Center

Brockway, Debra; Libera, Matthew; Welner, Heidi

2011-01-01

Creating slime fascinates students. Unfortunately, though intrigue is at its peak, the educational aspect of this activity is often minimal. This article describes a chemistry lab that closely relates to the slime lab and allows high school students to explore the concepts of chemical bonding, properties, and replacement reactions. It involves the…

Interactive, Online, Adsorption Lab to Support Discovery of the Scientific Process

NASA Astrophysics Data System (ADS)

Carroll, K. C.; Ulery, A. L.; Chamberlin, B.; Dettmer, A.

2014-12-01

Science students require more than methods practice in lab activities; they must gain an understanding of the application of the scientific process through lab work. Large classes, time constraints, and funding may limit student access to science labs, denying students access to the types of experiential learning needed to motivate and develop new scientists. Interactive, discovery-based computer simulations and virtual labs provide an alternative, low-risk opportunity for learners to engage in lab processes and activities. Students can conduct experiments, collect data, draw conclusions, and even abort a session. We have developed an online virtual lab, through which students can interactively develop as scientists as they learn about scientific concepts, lab equipment, and proper lab techniques. Our first lab topic is adsorption of chemicals to soil, but the methodology is transferrable to other topics. In addition to learning the specific procedures involved in each lab, the online activities will prompt exploration and practice in key scientific and mathematical concepts, such as unit conversion, significant digits, assessing risks, evaluating bias, and assessing quantity and quality of data. These labs are not designed to replace traditional lab instruction, but to supplement instruction on challenging or particularly time-consuming concepts. To complement classroom instruction, students can engage in a lab experience outside the lab and over a shorter time period than often required with real-world adsorption studies. More importantly, students can reflect, discuss, review, and even fail at their lab experience as part of the process to see why natural processes and scientific approaches work the way they do. Our Media Productions team has completed a series of online digital labs available at virtuallabs.nmsu.edu and scienceofsoil.com, and these virtual labs are being integrated into coursework to evaluate changes in student learning.

Object positioning in storages of robotized workcells using LabVIEW Vision

NASA Astrophysics Data System (ADS)

Hryniewicz, P.; Banaś, W.; Sękala, A.; Gwiazda, A.; Foit, K.; Kost, G.

2015-11-01

During the manufacturing process, each performed task is previously developed and adapted to the conditions and the possibilities of the manufacturing plant. The production process is supervised by a team of specialists because any downtime causes great loss of time and hence financial loss. Sensors used in industry for tracking and supervision various stages of a production process make it much easier to maintain it continuous. One of groups of sensors used in industrial applications are non-contact sensors. This group includes: light barriers, optical sensors, rangefinders, vision systems, and ultrasonic sensors. Through to the rapid development of electronics the vision systems were widespread as the most flexible type of non-contact sensors. These systems consist of cameras, devices for data acquisition, devices for data analysis and specialized software. Vision systems work well as sensors that control the production process itself as well as the sensors that control the product quality level. The LabVIEW program as well as the LabVIEW Vision and LabVIEW Builder represent the application that enables program the informatics system intended to process and product quality control. The paper presents elaborated application for positioning elements in a robotized workcell. Basing on geometric parameters of manipulated object or on the basis of previously developed graphical pattern it is possible to determine the position of particular manipulated elements. This application could work in an automatic mode and in real time cooperating with the robot control system. It allows making the workcell functioning more autonomous.

Bituminous Mixtures Lab

DOT National Transportation Integrated Search

2002-07-25

The Bituminous Mixtures Laboratory (BML) specializes in the research of asphalt pavement mixtures. This lab supports FHWA's efforts to develop, evaluate and improve materials, mixture design technology and performance-based tests for asphalt paving m...

My Brother’s Keeper National Lab Week

NASA Image and Video Library

2016-03-02

Students in the My Brother’s Keeper program watch as Jose Nunez of NASA Kennedy Space Center’s Exploration Research and Technology Programs demonstrates some of the hardware in the Electrostatic and Surface Physics Lab at the Florida spaceport. Kennedy is one of six NASA centers that participated in My Brother’s Keeper National Lab Week. The event is a nationwide effort to bring youth from underrepresented communities into federal labs and centers for hands-on activities, tours and inspirational speakers. Sixty students from the nearby cities of Orlando and Sanford visited Kennedy, where they toured the Vehicle Assembly Building, the Space Station Processing Facility and the center’s innovative Swamp Works Labs. The students also had a chance to meet and ask questions of a panel of subject matter experts from across Kennedy.

My Brother’s Keeper National Lab Week

NASA Image and Video Library

2016-03-02

Students in the My Brother’s Keeper program listen as Jose Nunez of NASA Kennedy Space Center’s Exploration Research and Technology Programs explains some of the hardware in the Electrostatic and Surface Physics Lab at the Florida spaceport. Kennedy is one of six NASA centers that participated in My Brother’s Keeper National Lab Week. The event is a nationwide effort to bring youth from underrepresented communities into federal labs and centers for hands-on activities, tours and inspirational speakers. Sixty students from the nearby cities of Orlando and Sanford visited Kennedy, where they toured the Vehicle Assembly Building, the Space Station Processing Facility and the center’s innovative Swamp Works Labs. The students also had a chance to meet and ask questions of a panel of subject matter experts from across Kennedy.

The effect of structural motifs on the ectodomain shedding of human angiotensin-converting enzyme.

PubMed

Conrad, Nailah; Schwager, Sylva L U; Carmona, Adriana K; Sturrock, Edward D

2016-12-02

Somatic angiotensin converting enzyme (sACE) is comprised of two homologous domains (N and C domains), whereas the smaller germinal isoform (tACE) is identical to the C domain. Both isozymes share an identical stalk, transmembrane and cytoplasmic domain, and undergo ectodomain shedding by an as yet unknown protease. Here we present evidence for the role of regions distal and proximal to the cleavage site in human ACE shedding. First, because of intrinsic differences between the N and C domains, discrete secondary structures (α-helix 7 and 8) on the surface of tACE were replaced with their N domain counterparts. Surprisingly, neither α-helix 7 nor α-helix 8 proved to be an absolute requirement for shedding. In the proximal ectodomain of tACE residues H 610 -L 614 were mutated to alanines and this resulted in a decrease in ACE shedding. An N-terminal extension of this mutation caused a reduction in cellular ACE activity. More importantly, it affected the processing of the protein to the membrane, resulting in expression of an underglycosylated form of ACE. When E 608 -H 614 was mutated to the homologous region of the N domain, processing was normal and shedding only moderately decreased suggesting that this region is more crucial for the processing of ACE than it is for regulating shedding. Finally, to determine whether glycosylation of the asparagine proximal to the Pro1199-Leu polymorphism in sACE affected shedding, the equivalent P 623 L mutation in tACE was investigated. The P 623 L tACE mutant showed an increase in shedding and MALDI MS analysis of a tryptic digest indicated that N 620 WT was glycosylated. The absence of an N-linked glycan at N 620 , resulted in an even greater increase in shedding. Thus, the conformational flexibility that the leucine confers to the stalk, is increased by the lack of glycosylation reducing access of the sheddase to the cleavage site. Copyright © 2016 Elsevier Inc. All rights reserved.

GeoLab: A Geological Workstation for Future Missions

NASA Technical Reports Server (NTRS)

Evans, Cynthia; Calaway, Michael; Bell, Mary Sue; Li, Zheng; Tong, Shuo; Zhong, Ye; Dahiwala, Ravi

2014-01-01

The GeoLab glovebox was, until November 2012, fully integrated into NASA's Deep Space Habitat (DSH) Analog Testbed. The conceptual design for GeoLab came from several sources, including current research instruments (Microgravity Science Glovebox) used on the International Space Station, existing Astromaterials Curation Laboratory hardware and clean room procedures, and mission scenarios developed for earlier programs. GeoLab allowed NASA scientists to test science operations related to contained sample examination during simulated exploration missions. The team demonstrated science operations that enhance theThe GeoLab glovebox was, until November 2012, fully integrated into NASA's Deep Space Habitat (DSH) Analog Testbed. The conceptual design for GeoLab came from several sources, including current research instruments (Microgravity Science Glovebox) used on the International Space Station, existing Astromaterials Curation Laboratory hardware and clean room procedures, and mission scenarios developed for earlier programs. GeoLab allowed NASA scientists to test science operations related to contained sample examination during simulated exploration missions. The team demonstrated science operations that enhance the early scientific returns from future missions and ensure that the best samples are selected for Earth return. The facility was also designed to foster the development of instrument technology. Since 2009, when GeoLab design and construction began, the GeoLab team [a group of scientists from the Astromaterials Acquisition and Curation Office within the Astromaterials Research and Exploration Science (ARES) Directorate at JSC] has progressively developed and reconfigured the GeoLab hardware and software interfaces and developed test objectives, which were to 1) determine requirements and strategies for sample handling and prioritization for geological operations on other planetary surfaces, 2) assess the scientific contribution of selective in-situ sample

Information-Theoretic Approach May Shed a Light to a Better Understanding and Sustaining the Integrity of Ecological-Societal Systems under Changing Climate

NASA Astrophysics Data System (ADS)

Kim, J.

2016-12-01

Considering high levels of uncertainty, epistemological conflicts over facts and values, and a sense of urgency, normal paradigm-driven science will be insufficient to mobilize people and nation toward sustainability. The conceptual framework to bridge the societal system dynamics with that of natural ecosystems in which humanity operates remains deficient. The key to understanding their coevolution is to understand `self-organization.' Information-theoretic approach may shed a light to provide a potential framework which enables not only to bridge human and nature but also to generate useful knowledge for understanding and sustaining the integrity of ecological-societal systems. How can information theory help understand the interface between ecological systems and social systems? How to delineate self-organizing processes and ensure them to fulfil sustainability? How to evaluate the flow of information from data through models to decision-makers? These are the core questions posed by sustainability science in which visioneering (i.e., the engineering of vision) is an essential framework. Yet, visioneering has neither quantitative measure nor information theoretic framework to work with and teach. This presentation is an attempt to accommodate the framework of self-organizing hierarchical open systems with visioneering into a common information-theoretic framework. A case study is presented with the UN/FAO's communal vision of climate-smart agriculture (CSA) which pursues a trilemma of efficiency, mitigation, and resilience. Challenges of delineating and facilitating self-organizing systems are discussed using transdisciplinary toold such as complex systems thinking, dynamic process network analysis and multi-agent systems modeling. Acknowledgments: This study was supported by the Korea Meteorological Administration Research and Development Program under Grant KMA-2012-0001-A (WISE project).

Membrane vesicles shed by oligodendroglioma cells induce neuronal apoptosis.

PubMed

D'Agostino, Stefania; Salamone, Monica; Di Liegro, Italia; Vittorelli, M Letizia

2006-11-01

In order to investigate the mechanism by which oligodendrogliomas cause neuronal damage, media conditioned by G26/24 oligodendroglioma cells, were fractionated into shed vesicles and vesicle-free supernatants, and added to primary cultures of rat fetal cortical neurons. After one night treatment with vesicles, a reproducible, dose-dependent, inhibitory effect on neurite outgrowth was already induced and, after 48-72 h of incubation, neuronal apoptosis was evident. Vesicle-free supernatants and vesicles shed by NIH-3T3 cells had no inhibitory effects on neurons. Western blot analyses showed that treated neurons expressed a decreased amount of neurofilament (NF), growth-associated protein (GAP-43) and microtubule-associated protein (MAP-2). Moreover procaspase-3 and -8 were activated while Bcl-2 expression was reduced. Vesicles were found positive for the proapoptotic molecule, Fas-ligand (Fas-L), and for the B isoform of Nogo protein, a myelin component with inhibitory effects on neurons. Nogo B involvement in the vesicle effects was analyzed both by testing the neutralizing capability of anti-Nogo antibodies and by removing the Nogo receptor from neurons by phospholipase C digestion. These treatments did not revert the vesicle effects. To test the role of Fas-L, vesicles were treated with functional anti-Fas-L monoclonals. Vesicle inhibitory and proapoptotic effects were reduced. Vesicles shed by ovarian carcinoma cells (OvCa), which are known to vehicle biologically active Fas-L, had similar effects on neurons to those of oligodendroglioma vesicles, and their inhibitory effects were also reduced by anti Fas-L antibodies. We therefore conclude that vesicles shed by G26/24 cells induce neuronal apoptosis at least partially by a Fas-L mediated mechanism.

Genital HSV Shedding among Kenyan Women Initiating Antiretroviral Therapy

PubMed Central

Manguro, Griffins O.; Masese, Linnet N.; Deya, Ruth W.; Magaret, Amalia; Wald, Anna; McClelland, R. Scott; Graham, Susan M.

2016-01-01

Objectives Genital ulcer disease (GUD) prevalence increases in the first month of antiretroviral treatment (ART), followed by a return to baseline prevalence by month 3. Since most GUD is caused by herpes simplex virus type 2 (HSV-2), we hypothesized that genital HSV detection would follow a similar pattern after treatment initiation. Methods We conducted a prospective cohort study of 122 HSV-2 and HIV-1 co-infected women with advanced HIV disease who initiated ART and were followed closely with collection of genital swab specimens for the first three months of treatment. Results At baseline, the HSV detection rate was 32%, without significant increase in genital HSV detection noted during the first month or the third month of ART. HIV-1 shedding declined during this period; no association was also noted between HSV and HIV-1 shedding during this period. Conclusion Because other studies have reported increased HSV detection in women initiating ART and we have previously reported an increase in GUD during early ART, it may be prudent to counsel HIV-1 infected women initiating ART that HSV shedding in the genital tract may continue after ART initiation. PMID:27683204

Experimentally simulating the dynamics of quantum light and matter at ultrastrong coupling using circuit QED (2) - light dynamics and light-matter entanglement -

NASA Astrophysics Data System (ADS)

Sagastizabal, R.; Langford, N. K.; Kounalakis, M.; Dickel, C.; Bruno, A.; Luthi, F.; Thoen, D. J.; Endo, A.; Dicarlo, L.

Light-matter interaction can lead to large photon build-up and hybrid atom-photon entanglement in the ultrastrong coupling (USC) regime, where the coupling strength becomes comparable to the eigenenergies of the system. Accessing the cavity degree of freedom, however, is an outstanding challenge in natural USC systems. In this talk, we directly probe light field dynamics in the USC regime using a digital simulation of the quantum Rabi model in a planar circuit QED chip with a transmon moderately coupled to a resonator. We produce high-accuracy USC light-matter dynamics, using second-order Trotterisation and up to 90 Trotter steps. We probe the average photon number, photon parity and perform Wigner tomography of the simulated field. Finally, we combine tomography of the resonator with qubit measurements to evidence the Schrödinger-cat-like atom-photon entanglement which is a key signature of light-matter dynamics in the USC regime. Funding from the EU FP7 Project ScaleQIT, the ERC Synergy Grant QC-lab, the Netherlands Organization of Scientic Research (NWO), and Microsoft Research.

CAD Instructor Designs Eco-Friendly Shed

ERIC Educational Resources Information Center

Schwendau, Mark

2013-01-01

Dissatisfied with the options offered by big box stores--and wanting to save some money and go as green as possible--the author puts his design and construction skills to good use. In this article, he shares how he designed and built an eco-friendly shed. He says he is very pleased with the results of working with his own design, reducing waste,…

Magnetic Viscous Drag for Friction Labs

ERIC Educational Resources Information Center

Gaffney, Chris; Catching, Adam

2016-01-01

The typical friction lab performed in introductory mechanics courses is usually not the favorite of either the student or the instructor. The measurements are not all that easy to make, and reproducibility is usually a troublesome issue. This paper describes the augmentation of such a friction lab with a study of the viscous drag on a magnet…

Detail of C.W.E. Storage Shed (Bldg. 126) monorail switches. Workers ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Detail of C.W.E. Storage Shed (Bldg. 126) monorail switches. Workers pulled the chains at right to align the switches. Workers also pulled the hoists, loaded or unloaded, along the monorail tracks - Atchison, Topeka, Santa Fe Railroad, Albuquerque Shops, C.W.E. Storage Shed, 908 Second Street, Southwest, Albuquerque, Bernalillo County, NM

Characterizing Scitillation and Cherenkov Light Yield in Water-Based Liquid Scintillators

NASA Astrophysics Data System (ADS)

Land, B. J.; Caravaca, J.; Descamps, F. B.; Orebi Gann, G. D.

2016-03-01

The recent development of Water-based Liquid Scintillator (WbLS) has made it possible to produce scintillating materials with highly tunable light yields and excellent optical clarity. This allows for a straightforward combination of the directional properties of Cherenkov light with the greater energy resolution afforded by the typically brighter scintillation light, which lends itself well to a broad program of neutrino physics. Here we explore the light yields and optical properties of WbLS materials in development for Theia (formerly ASDC) as measured in our benchtop Theia R&D at Berkeley Lab and extrapolate to larger detectors.

eduSPIM: Light Sheet Microscopy in the Museum.

PubMed

Jahr, Wiebke; Schmid, Benjamin; Weber, Michael; Huisken, Jan

2016-01-01

Light sheet microscopy (or selective plane illumination microscopy) is an important imaging technique in the life sciences. At the same time, this technique is also ideally suited for community outreach projects, because it produces visually appealing, highly dynamic images of living organisms and its working principle can be understood with basic optics knowledge. Still, the underlying concepts are widely unknown to the non-scientific public. On the occasion of the UNESCO International Year of Light, a technical museum in Dresden, Germany, launched a special, interactive exhibition. We built a fully functional, educational selective plane illumination microscope (eduSPIM) to demonstrate how developments in microscopy promote discoveries in biology. To maximize educational impact, we radically reduced a standard light sheet microscope to its essential components without compromising functionality and incorporated stringent safety concepts beyond those needed in the lab. Our eduSPIM system features one illumination and one detection path and a sealed sample chamber. We image fixed zebrafish embryos with fluorescent vasculature, because the structure is meaningful to laymen and visualises the optical principles of light sheet microscopy. Via a simplified interface, visitors acquire fluorescence and transmission data simultaneously. The universal concepts presented here may also apply to other scientific approaches that are communicated to laymen in interactive settings. The specific eduSPIM design is adapted easily for various outreach and teaching activities. eduSPIM may even prove useful for labs needing a simple SPIM. A detailed parts list and schematics to rebuild eduSPIM are provided.

Low flow vortex shedding flowmeter

NASA Technical Reports Server (NTRS)

Waugaman, Charles J.

1989-01-01

The purpose was to continue a development project on a no moving parts vortex shedding flowmeter used for flow measurement of hypergols. The project involved the design and construction of a test loop to evaluate the meter for flow of Freon which simulates the hypergol fluids. Results were obtained on the output frequency characteristics of the flow meter as a function of flow rate. A family of flow meters for larger size lines and ranges of flow was sized based on the results of the tested meter.

Berkeley Lab Scientist Named MacArthur "Genius" Fellow for Audio

Science.gov Websites

Preservation Research | Berkeley Lab Berkeley Lab A-Z Index Directory Submit Web People Navigation Berkeley Lab Search Submit Web People Close About the Lab Leadership/Organization Calendar News to digitally recover a 128-year-old recording of Alexander Graham Bell's voice, enabling people to

Axial-Current Matrix Elements in Light Nuclei from Lattice QCD

NASA Astrophysics Data System (ADS)

Savage, M.; Beane, S.; Chang, E.; Davoudi, Z.; Detmold, W.; Orginos, K.; Shanahan, P.; Tiburzi, B.; Wagman, M.; Winter, F.; Nplqcd Collaboration

I present results from the first lattice QCD calculations of axial-current matrix elements in light nuclei, performed by the NPLQCD collaboration. Precision calculations of these matrix elements, and the subsequent extraction of multi-nucleon axial-current operators, are essential in refining theoretical predictions of the proton-proton fusion cross section, neutrino-nucleus cross sections and $\\beta\\beta$-decay rates of nuclei. In addition, they are expected to shed light on the phenomenological quenching of $g_A$ that is required in nuclear many-body calculations.

Infant Mouse Model for the Study of Shedding and Transmission during Streptococcus pneumoniae Monoinfection

PubMed Central

Zafar, M. Ammar; Kono, Masamitsu; Wang, Yang; Zangari, Tonia

2016-01-01

One of the least understood aspects of the bacterium Streptococcus pneumoniae (pneumococcus) is its transmission from host to host, the critical first step in both the carrier state and the disease state. To date, transmission models have depended on influenza A virus coinfection, which greatly enhances pneumococcal shedding to levels that allow acquisition by a new host. Here, we describe an infant mouse model that can be utilized to study pneumococcal colonization, shedding, and transmission during bacterial monoinfection. Using this model, we demonstrated that the level of bacterial shedding is highest in pups infected intranasally at age 4 days and peaks over the first 4 days postchallenge. Shedding results differed among isolates of five different pneumococcal types. Colonization density was found to be a major factor in the level of pneumococcal shedding and required expression of capsule. Transmission within a litter occurred when there was a high ratio of colonized “index” pups to uncolonized “contact” pups. Transmission was observed for each of the well-colonizing pneumococcal isolates, with the rate of transmission proportional to the level of shedding. This model can be used to examine bacterial and host factors that contribute to pneumococcal transmission without the effects of viral coinfection. PMID:27400721

Optimization of light polarization sensitivity in QWIP detectors

NASA Astrophysics Data System (ADS)

Berurier, Arnaud; Nedelcu, Alexandru

2013-07-01

The current development of QWIPs (Quantum Well Infrared Photodetectors) at III-V Lab led to the production of 20 μm pitch, mid-format and full TV-format LWIR starring arrays with excellent performances, uniformity and stability. At the present time III-V Lab, together with TOL (Thales Optronics Ltd.) and SOFRADIR (Société Française de Détecteurs Infrarouges), work on the demonstration of a 20 μm pitch, 640 × 512 LWIR focal plane array (FPA) which detects the incident IR light polarization. Manufactured objects present a strong linear polarization signature in thermal emission. It is of high interest to achieve a detector able to measure precisely the degree of linear polarization, in order to distinguish artificial and natural objects in the observed scene. In this paper, we present a theoretical investigation of the optical coupling in polarization sensitive pixels. The QWIP modeling is performed by the Finite Difference Time Domain (FDTD) method. The aim is to optimize the sensitivity to light polarization as well as the performance of the detector.

Position sensitivity by light splitting in scintillator arrays

NASA Astrophysics Data System (ADS)

Bisplinghoff, J.; Bollmann, R.; Cloth, P.; Dohrmann, F.; Dorner, G.; Drüke, V.; Ernst, J.; Eversheim, P. D.; Filges, D.; Gasthuber, M.; Gebel, R.; Groβ, A.; Groβ-Hardt, R.; Hinterberger, F.; Jahn, R.; Kühl, L.; Lahr, U.; Langkau, R.; Lippert, G.; Mayer-Kuckuk, T.; Maschuw, R.; Mertler, G.; Metsch, B.; Mosel, F.; Paetz gen. Schieck, H.; Petry, H. R.; Prasuhn, D.; Przewoski, B. v.; Rohdjeβ, H.; Rosendaal, D.; Rossen, P. v.; Scheid, H.; Schirm, N.; Schwandt, F.; Scobel, W.; Sprute, L.; Stein, H.; Theis, D.; Weber, J.; Wiedmann, W.; Woller, K.; Ziegler, R.; EDDA Collaboration

1993-05-01

A novel detector design of overlapping plastic scintillator elements in cylindrical geometry has been developed for detection of low multiplicity events of fast protons and other light charged particles: each particle traversing the detector from the axis outwards will produce light in several elements. The relative amounts of energy deposited in those elements allow one to interpolate on the particle trajectory beyond the resolution given by the granularity. The detector covers the angular range 10° ≤ Θlab ≤ 72° and 0° ≤ ϕ ≤ 360° with an inner layer of scintillator bars of triangular cross section and an outer layer of rings. The material is BC408. Tests with minimum ionizing electron beams show that spatial resolutions of ΔΘlab ≈ 1.5° and Δϕ12 ≈ 1.5° (FWHM) can be obtained for electrons or proton pairs with energies in the GeV range. In the EDDA experiment the ultimate spatial resolution is then determined by the size of the interaction area rather than by the intrinsic pulse height resolution of the detector.

Quantifying shedding of synthetic fibers from textiles; a source of microplastics released into the environment.

PubMed

Carney Almroth, Bethanie M; Åström, Linn; Roslund, Sofia; Petersson, Hanna; Johansson, Mats; Persson, Nils-Krister

2018-01-01

Microplastics in the environment are a subject of intense research as they pose a potential threat to marine organisms. Plastic fibers from textiles have been indicated as a major source of this type of contaminant, entering the oceans via wastewater and diverse non-point sources. Their presence is also documented in terrestrial samples. In this study, the amount of microfibers shedding from synthetic textiles was measured for three materials (acrylic, nylon, polyester), knit using different gauges and techniques. All textiles were found to shed, but polyester fleece fabrics shed the greatest amounts, averaging 7360 fibers/m -2 /L -1 in one wash, compared with polyester fabrics which shed 87 fibers/m -2 /L -1 . We found that loose textile constructions shed more, as did worn fabrics, and high twist yarns are to be preferred for shed reduction. Since fiber from clothing is a potentially important source of microplastics, we suggest that smarter textile construction, prewashing and vacuum exhaustion at production sites, and use of more efficient filters in household washing machines could help mitigate this problem.

MODELING HUMAN EXPOSURES AND DOSE USING A 2-DIMENSIONAL MONTE-CARLO MODEL (SHEDS)

EPA Science Inventory

Since 1998, US EPA's National Exposure Research Laboratory (NERL) has been developing the Stochastic Human Exposure and Dose Simulation (SHEDS) model for various classes of pollutants. SHEDS is a physically-based probabilistic model intended for improving estimates of human ex...

Flexible HVAC System for Lab or Classroom.

ERIC Educational Resources Information Center

Friedan, Jonathan

2001-01-01

Discusses an effort to design a heating, ventilation, and air conditioning system flexible enough to accommodate an easy conversion of classrooms to laboratories and dry labs to wet labs. The design's energy efficiency and operations and maintenance are examined. (GR)

Distributed Load Shedding over Directed Communication Networks with Time Delays

DOE Office of Scientific and Technical Information (OSTI.GOV)

Yang, Tao; Wu, Di

When generation is insufficient to support all loads under emergencies, effective and efficient load shedding needs to be deployed in order to maintain the supply-demand balance. This paper presents a distributed load shedding algorithm, which makes efficient decision based on the discovered global information. In the global information discovery process, each load only communicates with its neighboring load via directed communication links possibly with arbitrarily large but bounded time varying communication delays. We propose a novel distributed information discovery algorithm based on ratio consensus. Simulation results are used to validate the proposed method.

The Role of Drag Force in Shedding of Multiple Sessile Drops

NASA Astrophysics Data System (ADS)

Razzaghi, Aysan; Banitabaei, Sayyed Hossein; Amirfazli, Alidad; -Team

2017-11-01

A sessile drop placed on a solid surface can shed, if the drag force due to a shearing airflow overcomes the drop adhesion to the surface. Sessile drop shedding is of importance due to its applications in condensation, fuel cells, icing, etc. Majority of the studies so far have considered the shedding of a single sessile droplet; however, in the applications above, multiple sessile droplets appear on a surface. Shedding of sessile drops in different arrangements, i.e. tandem, side by side, triangle, and rectangle have been investigated both experimentally and through VOF simulations. The minimum air velocity (Ucr) at which the drop(s) at the upstream dislodge from the surface was measured. Drops were placed in a wind tunnel with increasing air velocity at a rate of 1m/s2. It has been found that Ucr, deviates from its value for a single drop due to presence of the neighboring drops. The amount of the deviation is closely related to the flow pattern and interaction of drop wakes which are elucidated numerically. The interacting wakes change the drag force on the drops. Generally, the adhesion force is not affected by presence of other drops. As such, when the drops' wakes are interacting strongly, Ucr can increase by 45%.

Acoustic Resonance and Vortex Shedding from Tube Banks of Boiler Plant

NASA Astrophysics Data System (ADS)

Hamakawa, Hiromitsu; Matsue, Hiroto; Nishida, Eiichi; Fukano, Tohru

This paper focuses on the relationship between acoustic resonance and vortex shedding from the tube banks of a boiler plant. We have built a model similar to the actual boiler plant to clarify the characteristics of acoustic resonance phenomena and vortex shedding. The model used in-line tube banks with a small tube pitch ratio. We examined the relationship between the acoustic resonance of the actual plant and that of the model, and measured the sound pressure level, acoustic pressure mode shape, spectrum of velocity fluctuation, and gap velocity. Gap velocity was defined as the mean velocity in the smallest gaps between two neighboring tubes in the transverse direction. As a result, the resonant frequencies and mode shapes of the acoustic resonances in the actual boiler plant agreed well with those in the similar model. We found many peak frequencies in the sound pressure level spectrum when acoustic resonances occurred. The typical Strouhal numbers at the onset velocity of acoustic resonances were about 0.19, 0.26 and 0.52. Periodic velocity fluctuation caused by vortex shedding was observed inside the tube banks without acoustic resonance. The Strouhal number measured for vortex shedding was 0.15. Acoustic resonances of higher-order modes were generated in this plant.

Duration of shedding of respiratory syncytial virus in a community study of Kenyan children.

PubMed

Okiro, Emelda A; White, Lisa J; Ngama, Mwanajuma; Cane, Patricia A; Medley, Graham F; Nokes, D James

2010-01-22

Our understanding of the transmission dynamics of respiratory syncytial virus (RSV) infection will be better informed with improved data on the patterns of shedding in cases not limited only to hospital admissions. In a household study, children testing RSV positive by direct immunofluorescent antibody test (DFA) were enrolled. Nasal washings were scheduled right away, then every three days until day 14, every 7 days until day 28 and every 2 weeks until a maximum of 16 weeks, or until the first DFA negative RSV specimen. The relationship between host factors, illness severity and viral shedding was investigated using Cox regression methods. From 151 families a total of 193 children were enrolled with a median age of 21 months (range 1-164 months), 10% infants and 46% male. The rate of recovery from infection was 0.22/person/day (95% CI 0.19-0.25) equivalent to a mean duration of shedding of 4.5 days (95%CI 4.0-5.3), with a median duration of shedding of 4 days (IQR 2-6, range 1-14). Children with a history of RSV infection had a 40% increased rate of recovery i.e. shorter duration of viral shedding (hazard ratio 1.4, 95% CI 1.01-1.86). The rate of cessation of shedding did not differ significantly between males and females, by severity of infection or by age. We provide evidence of a relationship between the duration of shedding and history of infection, which may have a bearing on the relative role of primary versus re-infections in RSV transmission in the community.

Shedding light on El Farol

NASA Astrophysics Data System (ADS)

Challet, Damien; Marsili, M.; Ottino, Gabriele

2004-02-01

We mathematize El Farol bar problem and transform it into a workable model. We find general conditions on the predictor space under which the convergence of the average attendance to the resource level does not require any intelligence on the side of the agents. Secondly, specializing to a particular ensemble of continuous strategies yields a model similar to the Minority Game. Statistical physics of disordered systems allows us to derive a complete understanding of the complex behavior of this model, on the basis of its phase diagram.

Shedding Light, Reducing the Heat

ERIC Educational Resources Information Center

Perry, Mary

2006-01-01

Underlying the very heated debate over California's education budget for 2005-2006--and the battle over Proposition 76 on the November ballot--were two very different perspectives on school funding. These fundamental differences in perspective can stymie efforts to effect meaningful change. However, the Governor's Advisory Committee on Education…

Shedding light to sleep studies

NASA Astrophysics Data System (ADS)

Dieffenderfer, James; Krystal, Andrew; Bozkurt, Alper

2017-08-01

This paper presents our efforts in the development of a small wireless, flexible bandage sized near-infrared spectroscopy (NIRS) system for sleep analysis. The current size of the system is 2.8 cm × 1.7 cm × 0.6 cm. It is capable of performing NIRS with 660nm, 940nm and 850nm wavelengths for up to 11 hours continuously. The device is placed on the forehead to measure from the prefrontal cortex and the raw data is continuously streamed over Bluetooth to a nearby data aggregator such as a smartphone for post processing and cloud connection. In this study, we performed traditional polysomnography simultaneously with NIRS to evaluate agreement with traditional measures of sleep and to provide labelled data for future work involving learning algorithms. Ultimately, we expect a machine learning algorithm to be able to generate characterization of sleep states comparable to traditional methods based on this biophotonics data. The system also includes an inertial measurement unit and the features that can be extracted from the presented system include sleep posture, heart rate, respiratory rate, relative change in oxy and deoxy hemoglobin concentrations and tissue oxygenation and cerebral arterial oxygen extracted from these. Preliminary proof of concept results are promising and demonstrate the capability to measure heart rate, respiratory rate and slow-wave-sleep stages. This system serves as a prototype to evaluate the potential of a small bandage-size continuous-wave NIRS device to be a useful means of studying sleep.

Shedding Light on Solar Power

NASA Technical Reports Server (NTRS)

2002-01-01

Glenn Research Center sponsored an SBIR contract with ENTECH, in which the company worked to mold its successful terrestrial concentrator technology into applications that would generate solar power for space missions. ENTECH's first application made use of small, dome-shaped Fresnel lenses to direct sunlight onto high- efficiency photovoltaic cells. After some key adjustments, the mini- dome lens array was flown as part of the U.S. Air Force/NASA Photovoltaic Array Space Power Plus Diagnostics (PASP Plus) flight experiment in 1994. Due to their three-dimensional shape, the mini- dome lenses entailed construction by a batch molding process, which is naturally more costly than a continuous process. To overcome this disadvantage and meet the requirement for precise solar pointing in two axes, ENTECH started developing solar concentrator arrays for space using a line-focus lens that can be mass-produced by a continuous process. This new technology, named Solar Concentrator Array with Refractive Linear Element Technology (SCARLET), was created with support from Glenn and the Ballistic Missile Defense Organization, and was used to power the NASA/Jet Propulsion Laboratory Deep Space 1 spacecraft.

Shedding Light on Shadow Education

ERIC Educational Resources Information Center

Kobakhidze, Magda Nutsa

2015-01-01

This essay review examines four different movies that directly or indirectly refer to the theme of private tutoring or, as it is widely called, shadow education. The movies, directed in locations as diverse as India, Turkey, and Cambodia, are all made from a critical perspective. The directors demonstrate challenges in public education systems and…

[Shedding light on chaos theory].

PubMed

Chou, Shieu-Ming

2004-06-01

Gleick (1987) said that only three twentieth century scientific theories would be important enough to continue be of use in the twenty-first century: The Theory of Relativity, Quantum Theory, and Chaos Theory. Chaos Theory has become a craze which is being used to forge a new scientific system. It has also been extensively applied in a variety of professions. The purpose of this article is to introduce chaos theory and its nursing applications. Chaos is a sign of regular order. This is to say that chaos theory emphasizes the intrinsic potential for regular order within disordered phenomena. It is to be hoped that this article will inspire more nursing scientists to apply this concept to clinical, research, or administrative fields in our profession.

Shedding light on vampires: the phylogeny of vampyrellid amoebae revisited.

PubMed

Hess, Sebastian; Sausen, Nicole; Melkonian, Michael

2012-01-01

With the advent of molecular phylogenetic techniques the polyphyly of naked filose amoebae has been proven. They are interspersed in several supergroups of eukaryotes and most of them already found their place within the tree of life. Although the 'vampire amoebae' have attracted interest since the middle of the 19th century, the phylogenetic position and even the monophyly of this traditional group are still uncertain. In this study clonal co-cultures of eight algivorous vampyrellid amoebae and the respective food algae were established. Culture material was characterized morphologically and a molecular phylogeny was inferred using SSU rDNA sequence comparisons. We found that the limnetic, algivorous vampyrellid amoebae investigated in this study belong to a major clade within the Endomyxa Cavalier-Smith, 2002 (Cercozoa), grouping together with a few soil-dwelling taxa. They split into two robust clades, one containing species of the genus Vampyrella Cienkowski, 1865, the other containing the genus Leptophrys Hertwig & Lesser, 1874, together with terrestrial members. Supported by morphological data these clades are designated as the two families Vampyrellidae Zopf, 1885, and Leptophryidae fam. nov. Furthermore the order Vampyrellida West, 1901 was revised and now corresponds to the major vampyrellid clade within the Endomyxa, comprising the Vampyrellidae and Leptophryidae as well as several environmental sequences. In the light of the presented phylogenetic analyses morphological and ecological aspects, the feeding strategy and nutritional specialization within the vampyrellid amoebae are discussed.

Shedding light on proteins, nucleic acids, cells, humans and fish

NASA Technical Reports Server (NTRS)

Setlow, Richard B.

2002-01-01

I was trained as a physicist in graduate school. Hence, when I decided to go into the field of biophysics, it was natural that I concentrated on the effects of light on relatively simple biological systems, such as proteins. The wavelengths absorbed by the amino acid subunits of proteins are in the ultraviolet (UV). The wavelengths that affect the biological activities, the action spectra, also are in the UV, but are not necessarily parallel to the absorption spectra. Understanding these differences led me to investigate the action spectra for affecting nucleic acids, and the effects of UV on viruses and cells. The latter studies led me to the discovery of the important molecular nature of the damages affecting DNA (cyclobutane pyrimidine dimers) and to the discovery of nucleotide excision repair. Individuals with the genetic disease xeroderma pigmentosum (XP) are extraordinarily sensitive to sunlight-induced skin cancer. The finding, by James Cleaver, that their skin cells were defective in DNA repair strongly suggested that DNA damage was a key step in carcinogenesis. Such information was important for estimating the wavelengths in sunlight responsible for human skin cancer and for predicting the effects of ozone depletion on the incidence of non-melanoma skin cancer. It took experiments with backcross hybrid fish to call attention to the probable role of the longer UV wavelengths not absorbed by DNA in the induction of melanoma. These reflections trace the biophysicist's path from molecules to melanoma.

Shedding Light on Vampires: The Phylogeny of Vampyrellid Amoebae Revisited

PubMed Central

Hess, Sebastian; Sausen, Nicole; Melkonian, Michael

2012-01-01

With the advent of molecular phylogenetic techniques the polyphyly of naked filose amoebae has been proven. They are interspersed in several supergroups of eukaryotes and most of them already found their place within the tree of life. Although the ‘vampire amoebae’ have attracted interest since the middle of the 19th century, the phylogenetic position and even the monophyly of this traditional group are still uncertain. In this study clonal co-cultures of eight algivorous vampyrellid amoebae and the respective food algae were established. Culture material was characterized morphologically and a molecular phylogeny was inferred using SSU rDNA sequence comparisons. We found that the limnetic, algivorous vampyrellid amoebae investigated in this study belong to a major clade within the Endomyxa Cavalier-Smith, 2002 (Cercozoa), grouping together with a few soil-dwelling taxa. They split into two robust clades, one containing species of the genus Vampyrella Cienkowski, 1865, the other containing the genus Leptophrys Hertwig & Lesser, 1874, together with terrestrial members. Supported by morphological data these clades are designated as the two families Vampyrellidae Zopf, 1885, and Leptophryidae fam. nov. Furthermore the order Vampyrellida West, 1901 was revised and now corresponds to the major vampyrellid clade within the Endomyxa, comprising the Vampyrellidae and Leptophryidae as well as several environmental sequences. In the light of the presented phylogenetic analyses morphological and ecological aspects, the feeding strategy and nutritional specialization within the vampyrellid amoebae are discussed. PMID:22355342

Assessing Usage and Maximizing Finance Lab Impact: A Case Exploration

ERIC Educational Resources Information Center

Noguera, Magdy; Budden, Michael Craig; Silva, Alberto

2011-01-01

This paper reports the results of a survey conducted to assess students' usage and perceptions of a finance lab. Finance labs differ from simple computer labs as they typically contain data boards, streaming market quotes, terminals and software that allow for real-time financial analyses. Despite the fact that such labs represent significant and…

Design of Inquiry-Oriented Science Labs: Impacts on Students' Attitudes

ERIC Educational Resources Information Center

Baseya, J. M.; Francis, C. D.

2011-01-01

Background: Changes in lab style can lead to differences in learning. Two inquiry-oriented lab styles are guided inquiry (GI) and problem-based (PB). Students' attitudes towards lab are important to consider when choosing between GI and PB styles during curriculum design. Purpose: We examined the degree to which lab experiences are explained by a…

Determination of Shed Ice Particle Size Using High Speed Digital Imaging

NASA Technical Reports Server (NTRS)

Broughton, Howard; Owens, Jay; Sims, James J.; Bond, Thomas H.

1996-01-01

A full scale model of an aircraft engine inlet was tested at NASA Lewis Research Center's Icing Research Tunnel. Simulated natural ice sheds from the engine inlet lip were studied using high speed digital image acquisition and image analysis. Strategic camera placement integrated at the model design phase allowed the study of ice accretion on the inlet lip and the resulting shed ice particles at the aerodynamic interface plane at the rear of the inlet prior to engine ingestion. The resulting digital images were analyzed using commercial and proprietary software to determine the size of the ice particles that could potentially be ingested by the engine during a natural shedding event. A methodology was developed to calibrate the imaging system and insure consistent and accurate measurements of the ice particles for a wide range of icing conditions.

Designing virtual science labs for the Islamic Academy of Delaware

NASA Astrophysics Data System (ADS)

AlZahrani, Nada Saeed

Science education is a basic part of the curriculum in modern day classrooms. Instructional approaches to science education can take many forms but hands-on application of theory via science laboratory activities for the learner is common. Not all schools have the resources to provide the laboratory environment necessary for hands-on application of science theory. Some settings rely on technology to provide a virtual laboratory experience instead. The Islamic Academy of Delaware (IAD), a typical community-based organization, was formed to support and meet the essential needs of the Muslim community of Delaware. IAD provides science education as part of the overall curriculum, but cannot provide laboratory activities as part of the science program. Virtual science labs may be a successful model for students at IAD. This study was conducted to investigate the potential of implementing virtual science labs at IAD and to develop an implementation plan for integrating the virtual labs. The literature has shown us that the lab experience is a valuable part of the science curriculum (NBPTS, 2013, Wolf, 2010, National Research Council, 1997 & 2012). The National Research Council (2012) stressed the inclusion of laboratory investigations in the science curriculum. The literature also supports the use of virtual labs as an effective substitute for classroom labs (Babateen, 2011; National Science Teachers Association, 2008). Pyatt and Simms (2011) found evidence that virtual labs were as good, if not better than physical lab experiences in some respects. Although not identical in experience to a live lab, the virtual lab has been shown to provide the student with an effective laboratory experience in situations where the live lab is not possible. The results of the IAD teacher interviews indicate that the teachers are well-prepared for, and supportive of, the implementation of virtual labs to improve the science education curriculum. The investigator believes that with the

Transforming the advanced lab: Part I - Learning goals

NASA Astrophysics Data System (ADS)

Zwickl, Benjamin; Finkelstein, Noah; Lewandowski, H. J.

2012-02-01

Within the physics education research community relatively little attention has been given to laboratory courses, especially at the upper-division undergraduate level. As part of transforming our senior-level Optics and Modern Physics Lab at the University of Colorado Boulder we are developing learning goals, revising curricula, and creating assessments. In this paper, we report on the establishment of our learning goals and a surrounding framework that have emerged from discussions with a wide variety of faculty, from a review of the literature on labs, and from identifying the goals of existing lab courses. Our goals go beyond those of specific physics content and apparatus, allowing instructors to personalize them to their contexts. We report on four broad themes and associated learning goals: Modeling (math-physics-data connection, statistical error analysis, systematic error, modeling of engineered "black boxes"), Design (of experiments, apparatus, programs, troubleshooting), Communication, and Technical Lab Skills (computer-aided data analysis, LabVIEW, test and measurement equipment).

Student Plagiarism and Faculty Responsibility in Undergraduate Engineering Labs

ERIC Educational Resources Information Center

Parameswaran, Ashvin; Devi, Poornima

2006-01-01

In undergraduate engineering labs, lab reports are routinely copied. By ignoring this form of plagiarism, teaching assistants and lab technicians neglect their role responsibility. By designing courses that facilitate it, however inadvertently, professors neglect their causal responsibility. Using the case of one university, we show via interviews…

Scintillating fiber-based photon beam profiler for the Jefferson Lab tagged photon beam line

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zorn, C.; Barbosa, F.J.; Freyberger, A.

2000-10-01

A scintillating fiber hodoscope has been built for use as a photon beam profiler in the bremsstrahlung tagged photon beam in Hall B of the Thomas Jefferson National Accelerator Facility (Jefferson Lab). The device consists of a linear array of 64 2-2 mm2 scintillating fibers glued to a corresponding set of light guide fibers. Both fiber types use double-clad technology for maximum intensity. The light guide fibers are gently bent into a square array of holes and air-gap coupled to four compact position-sensitive photomultipliers (16 channel Hamamatsu R5900-M16). Custom electronics amplifies and converts the analog outputs to ECL pulses whichmore » are counted by VME-based scalars. The device consisting of the fibers, photomultipliers, and electronics is sealed within a light-tight aluminum box. Two modules make up a beam imaging 2-D system. The system has been tested successfully during an experimental run« less

Factors Associated with Shiga Toxin-Producing Escherichia coli Shedding by Dairy and Beef Cattle

PubMed Central

Venegas-Vargas, Cristina; Henderson, Scott; Khare, Akanksha; Mosci, Rebekah E.; Lehnert, Jonathan D.; Singh, Pallavi; Ouellette, Lindsey M.; Norby, Bo; Funk, Julie A.; Rust, Steven; Bartlett, Paul C.; Grooms, Daniel

2016-01-01

ABSTRACT Shiga toxin-producing Escherichia coli (STEC) is an important foodborne pathogen that can cause hemorrhagic colitis and hemolytic-uremic syndrome. Cattle are the primary reservoir for STEC, and food or water contaminated with cattle feces is the most common source of infections in humans. Consequently, we conducted a cross-sectional study of 1,096 cattle in six dairy herds (n = 718 animals) and five beef herds (n = 378 animals) in the summers of 2011 and 2012 to identify epidemiological factors associated with shedding. Fecal samples were obtained from each animal and cultured for STEC. Multivariate analyses were performed to identify risk factors associated with STEC positivity. The prevalence of STEC was higher in beef cattle (21%) than dairy cattle (13%) (odds ratio [OR], 1.76; 95% confidence interval [CI], 1.25, 2.47), with considerable variation occurring across herds (range, 6% to 54%). Dairy cattle were significantly more likely to shed STEC when the average temperature was >28.9°C 1 to 5 days prior to sampling (OR, 2.5; 95% CI, 1.25, 4.91), during their first lactation (OR, 1.8; 95% CI, 1.1, 2.8), and when they were <30 days in milk (OR, 3.9; 95% CI, 2.1, 7.2). These data suggest that the stress or the negative energy balance associated with lactation may result in increased STEC shedding frequencies in Michigan during the warm summer months. Future prevention strategies aimed at reducing stress during lactation or isolating high-risk animals could be implemented to reduce herd-level shedding levels and avoid transmission of STEC to susceptible animals and people. IMPORTANCE STEC shedding frequencies vary considerably across cattle herds in Michigan, and the shedding frequency of strains belonging to non-O157 serotypes far exceeds the shedding frequency of O157 strains, which is congruent with human infections in the state. Dairy cattle sampled at higher temperatures, in their first lactation, and early in the milk production stage were

Longitudinal Study on Oral Shedding of Herpes Simplex Virus 1 and Varicella-Zoster Virus in Individuals Infected with HIV

PubMed Central

van Velzen, Monique; Ouwendijk, Werner J.D.; Selke, Stacy; Pas, Suzan D.; van Loenen, Freek B.; Osterhaus, Albert D.M.E.; Wald, Anna; Verjans, Georges M.G.M.

2014-01-01

Primary herpes simplex virus 1 (HSV-1) and varicella-zoster virus (VZV) infection leads to a life-long latent infection of ganglia innervating the oral mucosa. HSV-1 and VZV reactivation is more common in immunocompromised individuals and may result in viral shedding in saliva. We determined the kinetics and quantity of oral HSV-1 and VZV shedding in HSV-1 and VZV seropositive individuals infected with HIV and to assess whether HSV-1 shedding involves reactivation of the same strain intra-individually. HSV-1 and VZV shedding was determined by real-time PCR of sequential daily oral swabs (n=715) collected for a median period of 31 days from 22 individuals infected with HIV. HSV-1 was genotyped by sequencing the viral thymidine kinase gene. Herpesvirus shedding was detected in 18 of 22 participants. Shedding of HSV-1 occurred frequently, on 14.3% of days, whereas solely VZV shedding was very rare. Two participants shed VZV. The median HSV-1 load was higher compared to VZV. HSV-1 DNA positive swabs clustered into 34 shedding episodes with a median duration of 2 days. The prevalence, duration and viral load of herpesvirus shedding did not correlate with CD4 counts and HIV load. The genotypes of the HSV-1 viruses shed were identical between and within shedding episodes of the same person, but were different between individuals. One-third of the individuals shed an HSV-1 strain potentially refractory to acyclovir therapy. Compared to HSV-1, oral VZV shedding is rare in individuals infected with HIV. Recurrent oral HSV-1 shedding is likely due to reactivation of the same latent HSV-1 strain. PMID:23780621

Using lab notebooks to examine students' engagement in modeling in an upper-division electronics lab course

NASA Astrophysics Data System (ADS)

Stanley, Jacob T.; Su, Weifeng; Lewandowski, H. J.

2017-12-01

We demonstrate how students' use of modeling can be examined and assessed using student notebooks collected from an upper-division electronics lab course. The use of models is a ubiquitous practice in undergraduate physics education, but the process of constructing, testing, and refining these models is much less common. We focus our attention on a lab course that has been transformed to engage students in this modeling process during lab activities. The design of the lab activities was guided by a framework that captures the different components of model-based reasoning, called the Modeling Framework for Experimental Physics. We demonstrate how this framework can be used to assess students' written work and to identify how students' model-based reasoning differed from activity to activity. Broadly speaking, we were able to identify the different steps of students' model-based reasoning and assess the completeness of their reasoning. Varying degrees of scaffolding present across the activities had an impact on how thoroughly students would engage in the full modeling process, with more scaffolded activities resulting in more thorough engagement with the process. Finally, we identified that the step in the process with which students had the most difficulty was the comparison between their interpreted data and their model prediction. Students did not use sufficiently sophisticated criteria in evaluating such comparisons, which had the effect of halting the modeling process. This may indicate that in order to engage students further in using model-based reasoning during lab activities, the instructor needs to provide further scaffolding for how students make these types of experimental comparisons. This is an important design consideration for other such courses attempting to incorporate modeling as a learning goal.

Reliability Constrained Priority Load Shedding for Aerospace Power System Automation

NASA Technical Reports Server (NTRS)

Momoh, James A.; Zhu, Jizhong; Kaddah, Sahar S.; Dolce, James L. (Technical Monitor)

2000-01-01

The need for improving load shedding on board the space station is one of the goals of aerospace power system automation. To accelerate the optimum load-shedding functions, several constraints must be involved. These constraints include congestion margin determined by weighted probability contingency, component/system reliability index, generation rescheduling. The impact of different faults and indices for computing reliability were defined before optimization. The optimum load schedule is done based on priority, value and location of loads. An optimization strategy capable of handling discrete decision making, such as Everett optimization, is proposed. We extended Everett method to handle expected congestion margin and reliability index as constraints. To make it effective for real time load dispatch process, a rule-based scheme is presented in the optimization method. It assists in selecting which feeder load to be shed, the location of the load, the value, priority of the load and cost benefit analysis of the load profile is included in the scheme. The scheme is tested using a benchmark NASA system consisting of generators, loads and network.

Behavior of variable V3 region from 16S rDNA of lactic acid bacteria in denaturing gradient gel electrophoresis.

PubMed

Ercolini, D; Moschetti, G; Blaiotta, G; Coppola, S

2001-03-01

Separation of amplified V3 region from 16S rDNA by denaturing gradient gel electrophoresis (DGGE) was tested as a tool for differentiation of lactic acid bacteria commonly isolated from food. Variable V3 regions of 21 reference strains and 34 wild strains referred to species belonging to the genera Pediococcus, Enterococcus, Lactococcus, Lactobacillus, Leuconostoc, Weissella, and Streptococcus were analyzed. DGGE profiles obtained were species-specific for most of the cultures tested. Moreover, it was possible to group the remaining LAB reference strains according to the migration of their 16S V3 region in the denaturing gel. The results are discussed with reference to their potential in the analysis of LAB communities in food, besides shedding light on taxonomic aspects.

Effectiveness of a Lab Manual Delivered on CD-ROM

ERIC Educational Resources Information Center

Brickman, Peggy; Ketter, Catherine A. Teare; Pereira, Monica

2005-01-01

Although electronic instructional media are becoming increasingly prevalent in science classrooms, their worth remains unproven. Here, student perceptions and performance using CD-ROM delivery of lab materials are assessed. Numerous learning barriers that produced lower lab grades for students using a CD-ROM lab manual in comparison to a print…

Axial-Current Matrix Elements in Light Nuclei from Lattice QCD

DOE Office of Scientific and Technical Information (OSTI.GOV)

Savage, Martin; Shanahan, Phiala E.; Tiburzi, Brian C.

2016-12-01

I present results from the first lattice QCD calculations of axial-current matrix elements in light nuclei, performed by the NPLQCD collaboration. Precision calculations of these matrix elements, and the subsequent extraction of multi-nucleon axial-current operators, are essential in refining theoretical predictions of the proton-proton fusion cross section, neutrino-nucleus cross sections andmore » $$\\beta\\beta$$-decay rates of nuclei. In addition, they are expected to shed light on the phenomenological quenching of $$g_A$$ that is required in nuclear many-body calculations.« less

Duration of shedding of respiratory syncytial virus in a community study of Kenyan children

PubMed Central

2010-01-01

Background Our understanding of the transmission dynamics of respiratory syncytial virus (RSV) infection will be better informed with improved data on the patterns of shedding in cases not limited only to hospital admissions. Methods In a household study, children testing RSV positive by direct immunofluorescent antibody test (DFA) were enrolled. Nasal washings were scheduled right away, then every three days until day 14, every 7 days until day 28 and every 2 weeks until a maximum of 16 weeks, or until the first DFA negative RSV specimen. The relationship between host factors, illness severity and viral shedding was investigated using Cox regression methods. Results From 151 families a total of 193 children were enrolled with a median age of 21 months (range 1-164 months), 10% infants and 46% male. The rate of recovery from infection was 0.22/person/day (95% CI 0.19-0.25) equivalent to a mean duration of shedding of 4.5 days (95%CI 4.0-5.3), with a median duration of shedding of 4 days (IQR 2-6, range 1-14). Children with a history of RSV infection had a 40% increased rate of recovery i.e. shorter duration of viral shedding (hazard ratio 1.4, 95% CI 1.01-1.86). The rate of cessation of shedding did not differ significantly between males and females, by severity of infection or by age. Conclusion We provide evidence of a relationship between the duration of shedding and history of infection, which may have a bearing on the relative role of primary versus re-infections in RSV transmission in the community. PMID:20096106

Plastic lab-on-a-chip for fluorescence excitation with integrated organic semiconductor lasers.

PubMed

Vannahme, Christoph; Klinkhammer, Sönke; Lemmer, Uli; Mappes, Timo

2011-04-25

Laser light excitation of fluorescent markers offers highly sensitive and specific analysis for bio-medical or chemical analysis. To profit from these advantages for applications in the field or at the point-of-care, a plastic lab-on-a-chip with integrated organic semiconductor lasers is presented here. First order distributed feedback lasers based on the organic semiconductor tris(8-hydroxyquinoline) aluminum (Alq3) doped with the laser dye 4-dicyanomethylene-2-methyl-6-(p-dimethylaminostyril)-4H-pyrane (DCM), deep ultraviolet induced waveguides, and a nanostructured microfluidic channel are integrated into a poly(methyl methacrylate) (PMMA) substrate. A simple and parallel fabrication process is used comprising thermal imprint, DUV exposure, evaporation of the laser material, and sealing by thermal bonding. The excitation of two fluorescent marker model systems including labeled antibodies with light emitted by integrated lasers is demonstrated.

California State University, Northridge: Hybrid Lab Courses

ERIC Educational Resources Information Center

EDUCAUSE, 2014

2014-01-01

California State University, Northridge's Hybrid Lab course model targets high failure rate, multisection, gateway courses in which prerequisite knowledge is a key to success. The Hybrid Lab course model components incorporate interventions and practices that have proven successful at CSUN and other campuses in supporting students, particularly…

The Portable Usability Testing Lab: A Flexible Research Tool.

ERIC Educational Resources Information Center

Hale, Michael E.; And Others

A group of faculty at the University of Georgia obtained funding for a research and development facility called the Learning and Performance Support Laboratory (LPSL). One of the LPSL's primary needs was obtaining a portable usability lab for software testing, so the facility obtained the "Luggage Lab 2000." The lab is transportable to…

Modifying Cookbook Labs.

ERIC Educational Resources Information Center

Clark, Robert, L.; Clough, Michael P.; Berg, Craig A.

2000-01-01

Modifies an extended lab activity from a cookbook approach for determining the percent mass of water in copper sulfate pentahydrate crystals to one which incorporates students' prior knowledge, engenders active mental struggling with prior knowledge and new experiences, and encourages metacognition. (Contains 12 references.) (ASK)

Water analysis in a lab-on-a-chip system

NASA Astrophysics Data System (ADS)

Freimuth, Herbert; von Germar, Frithjof; Frese, Ines; Nahrstedt, Elzbieta; Küpper, Michael; Schenk, Rainer; Baser, Björn; Ott, Johannes; Drese, Klaus; Detemple, Peter; Doll, Theodor

2006-01-01

The development of a lab-on-chip system which allows the parallel detection of a variety of different parameters of a water sample is presented. Water analysis typically comprises the determination of around 30 physical and chemical parameters. An even larger number can arise when special contaminations of organic molecules are of interest. A demonstration system has been realised to show the feasibility and performance of an integrated device for the determination of physical quantities like electrical conductivity, light absorption and turbidity. Additionally, chemical quantities like the pH-value and the content of inorganic and organic contaminations are also determined. Two chips of credit card size contain the analytical functions and will be fabricated by injection moulding. First prototypes have been manufactured by milling or precision milling for the optical components.

Men with disabilities - A cross sectional survey of health promotion, social inclusion and participation at community Men's Sheds.

PubMed

Wilson, Nathan J; Cordier, Reinie; Parsons, Richard; Vaz, Sharmila; Buchanan, Angus

2016-01-01

The intersections between chronicity, disability and social inequality are well understood. Novel ways to counter the social determinants of health and disability are needed. Men's Sheds are a community space where men can participate in a range of shared activities and potentially experience a health and social benefits. This cross-sectional survey was conducted to inform future research by determining who attended Men's Sheds and the range of health, social, community, and educational activities undertaken there. This paper explores the membership of people with disabilities (PWD) at Men's Sheds and the factors that predict their membership. An online survey link was sent to all known Men's Sheds internationally in 2012. Data were analyzed using descriptive and inferential (univariate and multivariate) statistics. 32.2% of international sheds and 29% of Australian sheds specifically targeted the inclusion of PWD. 80% of these sheds have significantly more members with disabilities than sheds who do no target PWD. Factors associated with greater membership of PWD included the provision of transport, social outings and promoting occupational skills. PWD are being encouraged to join and are joining Men's Sheds. This is significant as the value of participation and inclusion toward better health and wellbeing is well known. Men's Sheds offer a community space where the social determinants of chronicity and disability can potentially be countered. Copyright © 2016 Elsevier Inc. All rights reserved.

Spanish-Speaking Limited English Proficiency Patients and Call Light Use.

PubMed

Montie, Mary; Galinato, Jose Gabriel; Patak, Lance; Titler, Marita

2016-06-01

Despite a continuous increase of the limited English proficiency (LEP) population in the United States, disparities in the quality of care received in health care systems persist. This qualitative study explores the perceptions of hospitalized LEP patients on their call light use, as well as their perceptions of a prototype of a new multilingual call light system, Eloquence™. Individual interviews were conducted with 10 Spanish-speaking patient participants. Using a constant comparative method, the following themes emerged: (1) reasons for call light use, (2) challenges with communication, (3) patients' adaptation to language barriers, (4) perceived staff responses to call light, and (5) responses to the Eloquence™ demonstration. Data from this study shed light on the patient care experience of LEP patients and can help guide clinicians and administrators in providing culturally and linguistically competent care. © The Author(s) 2016.

Public Lab: Community-Based Approaches to Urban and Environmental Health and Justice.

PubMed

Rey-Mazón, Pablo; Keysar, Hagit; Dosemagen, Shannon; D'Ignazio, Catherine; Blair, Don

2018-06-01

This paper explores three cases of Do-It-Yourself, open-source technologies developed within the diverse array of topics and themes in the communities around the Public Laboratory for Open Technology and Science (Public Lab). These cases focus on aerial mapping, water quality monitoring and civic science practices. The techniques discussed have in common the use of accessible, community-built technologies for acquiring data. They are also concerned with embedding collaborative and open source principles into the objects, tools, social formations and data sharing practices that emerge from these inquiries. The focus is on developing processes of collaborative design and experimentation through material engagement with technology and issues of concern. Problem-solving, here, is a tactic, while the strategy is an ongoing engagement with the problem of participation in its technological, social and political dimensions especially considering the increasing centralization and specialization of scientific and technological expertise. The authors also discuss and reflect on the Public Lab's approach to civic science in light of ideas and practices of citizen/civic veillance, or "sousveillance", by emphasizing people before data, and by investigating the new ways of seeing and doing that this shift in perspective might provide.

Highly efficient light management for perovskite solar cells

NASA Astrophysics Data System (ADS)

Wang, Dong-Lin; Cui, Hui-Juan; Hou, Guo-Jiao; Zhu, Zhen-Gang; Yan, Qing-Bo; Su, Gang

2016-01-01

Organic-inorganic halide perovskite solar cells have enormous potential to impact the existing photovoltaic industry. As realizing a higher conversion efficiency of the solar cell is still the most crucial task, a great number of schemes were proposed to minimize the carrier loss by optimizing the electrical properties of the perovskite solar cells. Here, we focus on another significant aspect that is to minimize the light loss by optimizing the light management to gain a high efficiency for perovskite solar cells. In our scheme, the slotted and inverted prism structured SiO2 layers are adopted to trap more light into the solar cells, and a better transparent conducting oxide layer is employed to reduce the parasitic absorption. For such an implementation, the efficiency and the serviceable angle of the perovskite solar cell can be promoted impressively. This proposal would shed new light on developing the high-performance perovskite solar cells.

Highly efficient light management for perovskite solar cells.

PubMed

Wang, Dong-Lin; Cui, Hui-Juan; Hou, Guo-Jiao; Zhu, Zhen-Gang; Yan, Qing-Bo; Su, Gang

2016-01-06

Organic-inorganic halide perovskite solar cells have enormous potential to impact the existing photovoltaic industry. As realizing a higher conversion efficiency of the solar cell is still the most crucial task, a great number of schemes were proposed to minimize the carrier loss by optimizing the electrical properties of the perovskite solar cells. Here, we focus on another significant aspect that is to minimize the light loss by optimizing the light management to gain a high efficiency for perovskite solar cells. In our scheme, the slotted and inverted prism structured SiO2 layers are adopted to trap more light into the solar cells, and a better transparent conducting oxide layer is employed to reduce the parasitic absorption. For such an implementation, the efficiency and the serviceable angle of the perovskite solar cell can be promoted impressively. This proposal would shed new light on developing the high-performance perovskite solar cells.

Mobility and Transportation Analysis in Support of the Light Attack Battalion (LAB) Study. Volume 1. Mobility and Transportation Analyses

DTIC Science & Technology

1983-05-01

LAB) STUDY VOLUME I: MOBILITY AND TRANSPORTATION ANALYSES JAMES C EMANUEL L. ALAN DORNEY LAWRENCE E. MARTIN WAYNE E. FERGUSON JOHN P. O’MALLEY...Runs were maBe on dry, eIcha!londi?Ln0Ve^^rfaireS *? 0btain data 5n maximm attainable speeds under K?nn M? wISiI Addltional ana yses were

Reactivation and shedding of cytomegalovirus in astronauts during spaceflight

NASA Technical Reports Server (NTRS)

Mehta, S. K.; Stowe, R. P.; Feiveson, A. H.; Tyring, S. K.; Pierson, D. L.

2000-01-01

The reactivation of cytomegalovirus (CMV) in 71 astronauts was investigated, using polymerase chain reaction. A significantly greater (P<.0001) shedding frequency was found in urine samples from astronauts before spaceflight (10.6%) than in urine from the healthy control subject group (1.2%). Two of 4 astronauts studied during spaceflight shed CMV in urine. A significant increase (P<.0001) in CMV antibody titer, compared with baseline values, was also found 10 days before spaceflight. CMV antibody titer was further increased (P<.001) 3 days after landing, compared with 10 days before the mission. Significant increases in stress hormones were also found after landing. These results demonstrate that CMV reactivation occurred in astronauts before spaceflight and indicate that CMV may further reactivate during spaceflight.

Rotavirus shedding following administration of RV3-BB human neonatal rotavirus vaccine.

PubMed

Cowley, Daniel; Boniface, Karen; Bogdanovic-Sakran, Nada; Kirkwood, Carl D; Bines, Julie E

2017-08-03

The RV3-BB human neonatal rotavirus vaccine aims to provide protection from severe rotavirus disease from birth. A phase IIa safety and immunogenicity trial was undertaken in Dunedin, New Zealand between January 2012 and April 2014. Healthy, full-term (≥ 36 weeks gestation) babies, who were 0-5 d old were randomly assigned (1:1:1) to receive 3 doses of oral RV3-BB vaccine with the first dose given at 0-5 d after birth (neonatal schedule), or the first dose given at about 8 weeks after birth (infant schedule), or to receive placebo (placebo schedule). Vaccine take (serum immune response or stool shedding of vaccine virus after any dose) was detected after 3 doses of RV3-BB vaccine in >90% of participants when the first dose was administered in the neonatal and infant schedules. The aim of the current study was to characterize RV3-BB shedding and virus replication following administration of RV3-BB in a neonatal and infant vaccination schedule. Shedding was defined as detection of rotavirus by VP6 reverse transcription polymerase chain reaction (RT-PCR) in stool on days 3-7 after administration of RV3-BB. Shedding of rotavirus was highest following vaccination at 8 weeks of age in both neonatal and infant schedules (19/30 and 17/27, respectively). Rotavirus was detected in stool on days 3-7, after at least one dose of RV3-BB, in 70% (21/30) of neonate, 78% (21/27) of infant and 3% (1/32) placebo participants. In participants who shed RV3-BB, rotavirus was detectable in stool on day 1 following RV3-BB administration and remained positive until day 4-5 after administration. The distinct pattern of RV3-BB stool viral load demonstrated using a NSP3 quantitative qRT-PCR in participants who shed RV3-BB, suggests that detection of RV3-BB at day 3-7 was the result of replication rather than passage through the gastrointestinal tract.

Influence of On-farm pig Salmonella status on Salmonella Shedding at Slaughter.

PubMed

Casanova-Higes, A; Andrés-Barranco, S; Mainar-Jaime, R C

2017-08-01

The risk of Salmonella shedding among pigs at slaughter with regard to their previous on-farm Salmonella status was assessed in a group of pigs from a farm from NE of Spain. A total of 202 pigs that had been serologically monitored monthly during the fattening period and from which mesenteric lymph nodes (MLN) and faecal (SFEC) samples were collected at slaughter for Salmonella isolation were included. A repeated-measures anova was used to assess the relationship between mean OD% values during the fattening period and sampling time and bacteriology on MLN and SFEC. Pigs were also grouped into four groups, that is pigs seronegative during the fattening period and Salmonella negative in MLN (group A; n = 69); pigs seronegative during the fattening period but Salmonella positive in MLN (B; n = 36); pigs seropositive at least once and Salmonella positive in MLN (C; n = 50); and pigs seropositive at least once but Salmonella negative in (D; n = 47). Pigs shedding at slaughter seroconverted much earlier and showed much higher mean OD% values than non-shedders pigs. The proportion of Salmonella shedders in groups A and D was high and similar (26.1% and 29.8%, respectively), but significantly lower than that for groups B and C. The odds of shedding Salmonella for groups B and C were 4.8 (95% CI = 1.5-15.5) and 20.9 (3.7-118) times higher, respectively, when compared to A. It was concluded that a large proportion of Salmonella seronegative pigs may shed Salmonella at slaughter, which would be likely associated to previous exposure with contaminated environments (i.e. transport and lairage). For pigs already infected at farm, the likelihood of shedding Salmonella was much higher and may depend on whether the bacterium has colonized the MLN or not. The odds of shedding Salmonella spp. were always much higher for pigs in which Salmonella was isolated from MLN. © 2016 Blackwell Verlag GmbH.

Zinc Up-Regulates Insulin Secretion from β Cell-Like Cells Derived from Stem Cells from Human Exfoliated Deciduous Tooth (SHED).

PubMed

Kim, Gyuyoup; Shin, Ki-Hyuk; Pae, Eung-Kwon

2016-12-13

Stem cells from human exfoliated deciduous tooth (SHED) offer several advantages over other stem cell sources. Using SHED, we examined the roles of zinc and the zinc uptake transporter ZIP8 (Zrt- and irt-like protein 8) while inducing SHED into insulin secreting β cell-like stem cells (i.e., SHED-β cells). We observed that ZIP8 expression increased as SHED differentiated into SHED-β cells, and that zinc supplementation at day 10 increased the levels of most pancreatic β cell markers-particularly Insulin and glucose transporter 2 (GLUT2). We confirmed that SHED-β cells produce insulin successfully. In addition, we note that zinc supplementation significantly increases insulin secretion with a significant elevation of ZIP8 transporters in SHED-β cells. We conclude that SHED can be converted into insulin-secreting β cell-like cells as zinc concentration in the cytosol is elevated. Insulin production by SHED-β cells can be regulated via modulation of zinc concentration in the media as ZIP8 expression in the SHED-β cells increases.

Traditional Labs + New Questions = Improved Student Performance.

ERIC Educational Resources Information Center

Rezba, Richard J.; And Others

1992-01-01

Presents three typical lab activities involving the breathing rate of fish, the behavior of electromagnets, and tests for water hardness to demonstrate how labs can be modified to teach process skills. Discusses how basic concepts about experimentation are developed and ways of generating and improving science experiments. Includes a laboratory…

Comparison of Coxiella burnetii shedding in milk of dairy bovine, caprine, and ovine herds.

PubMed

Rodolakis, A; Berri, M; Héchard, C; Caudron, C; Souriau, A; Bodier, C C; Blanchard, B; Camuset, P; Devillechaise, P; Natorp, J C; Vadet, J P; Arricau-Bouvery, N

2007-12-01

The shedding of Coxiella burnetii in bovine, caprine, and ovine milk was measured using PCR, in 3 herds for each species, the bulk tank milk samples of which were positive at the time of their selection. Milk samples of 95 cows, 120 goats, and 90 ewes were sampled over 16 wk, as was the bulk tank milk. The shedding of C. burnetii in vaginal mucus and feces was checked at the beginning of the experiment and 2 mo later. The clinical signs in the selected herds as well as the duration and the shedding routes differed among the 3 species. The cows were asymptomatic and shed C. burnetii almost exclusively in milk. In one of the caprine herds, abortions due to C. burnetii were reported. The goats excreted the bacteria mainly in milk. In contrast, the ewes, which came from flocks with abortions due to Q fever (C. burnetii infection), shed the bacteria mostly in feces and in vaginal mucus. This could explain why human outbreaks of Q fever are more often related to ovine flocks than to bovine herds. These excretions did not seem more frequent when the samples were taken close to parturition. The samples were taken from 0 to 421 d after parturition in bovine herds and from 5 to 119 d and 11 to 238 d after parturition in the caprine and ovine herds, respectively. The shedding in milk was sometimes intermittent, and several animals shed the bacteria but were negative by ELISA: 80% of the ewes were seronegative, underscoring the lack of sensitivity of the ELISA tests available for veterinary diagnosis. The detection of antibodies in milk seems more sensitive than it is in serum.

A Well-Maintained Lab Is a Safer Lab. Safety Spotlight

ERIC Educational Resources Information Center

Walls, William H.; Strimel, Greg J.

2018-01-01

Administration and funding can cause Engineering/Technology Education (ETE) programs to thrive or die. To administrators, the production/prototyping equipment and laboratory setting are often viewed as the features that set ETE apart from other school subjects. A lab is a unique gift as well as a responsibility. If an administrator can see that…

Role of quantum correlations in light-matter quantum heat engines

NASA Astrophysics Data System (ADS)

Barrios, G. Alvarado; Albarrán-Arriagada, F.; Cárdenas-López, F. A.; Romero, G.; Retamal, J. C.

2017-11-01

We study a quantum Otto engine embedding a working substance composed of a two-level system interacting with a harmonic mode. The physical properties of the substance are described by a generalized quantum Rabi model arising in superconducting circuit realizations. We show that light-matter quantum correlation reduction during the hot bath stage and adiabatic stages act as an indicator for enhanced work extraction and efficiency, respectively. Also, we demonstrate that the anharmonic spectrum of the working substance has a direct impact on the transition from heat engine into refrigerator as the light-matter coupling is increased. These results shed light on the search for optimal conditions in the performance of quantum heat engines.

MatLab Programming for Engineers Having No Formal Programming Knowledge

NASA Technical Reports Server (NTRS)

Shaykhian, Linda H.; Shaykhian, Gholam Ali

2007-01-01

MatLab is one of the most widely used very high level programming languages for Scientific and engineering computations. It is very user-friendly and needs practically no formal programming knowledge. Presented here are MatLab programming aspects and not just the MatLab commands for scientists and engineers who do not have formal programming training and also have no significant time to spare for learning programming to solve their real world problems. Specifically provided are programs for visualization. Also, stated are the current limitations of the MatLab, which possibly can be taken care of by Mathworks Inc. in a future version to make MatLab more versatile.

eComLab: remote laboratory platform

NASA Astrophysics Data System (ADS)

Pontual, Murillo; Melkonyan, Arsen; Gampe, Andreas; Huang, Grant; Akopian, David

2011-06-01

Hands-on experiments with electronic devices have been recognized as an important element in the field of engineering to help students get familiar with theoretical concepts and practical tasks. The continuing increase the student number, costly laboratory equipment, and laboratory maintenance slow down the physical lab efficiency. As information technology continues to evolve, the Internet has become a common media in modern education. Internetbased remote laboratory can solve a lot of restrictions, providing hands-on training as they can be flexible in time and the same equipment can be shared between different students. This article describes an on-going remote hands-on experimental radio modulation, network and mobile applications lab project "eComLab". Its main component is a remote laboratory infrastructure and server management system featuring various online media familiar with modern students, such as chat rooms and video streaming.

Modeling of low-finesse, extrinsic fiber optic Fabry-Perot white light interferometers

NASA Astrophysics Data System (ADS)

Ma, Cheng; Tian, Zhipeng; Wang, Anbo

2012-06-01

This article introduces an approach for modeling the fiber optic low-finesse extrinsic Fabry-Pérot Interferometers (EFPI), aiming to address signal processing problems in EFPI demodulation algorithms based on white light interferometry. The main goal is to seek physical interpretations to correlate the sensor spectrum with the interferometer geometry (most importantly, the optical path difference). Because the signal demodulation quality and reliability hinge heavily on the understanding of such relationships, the model sheds light on optimizing the sensor performance.

Commerce Lab - A program of commercial flight opportunities

NASA Technical Reports Server (NTRS)

Robertson, J.; Atkins, H. L.; Williams, J. R.

1985-01-01

Commerce Lab is conceived as an adjunct to the National Space Transportation System (NSTS) by providing a focal point for commercial missions which could utilize existing NSTS carrier and resource capabilities for on-orbit experimentation in the microgravity sciences. In this context, the Commerce Lab program provides mission planning for private sector involvement in the space program, in general, and the commercial exploitation of the microgravity environment for materials processing research and development. It is expected that Commerce Lab will provide a logical transition between currently planned NSTS missions and future microgravity science and commercial R&D missions centered around the Space Station. The present study identifies candidate Commerce Lab flight experiments and their development status and projects a mission traffic model that can be used in commercial mission planning.

Searching for dark photon with positrons at Jefferson lab

NASA Astrophysics Data System (ADS)

Marsicano, Luca

2018-05-01

The interest in the Dark Photon (A' or U) has recently grown, since it could act as a light mediator to a new sector of Dark Matter particles. In this paradigm, the electron-positron annihilation can rarely produce a γA' pair. Various experiments (e.g. PADME@LNF [1], VEPP-3 [2]) have been proposed to detect this process using positron beams impinging on fixed targets. In such experiments, the energy of the photon from the e+e-→ γA' process is measured with an electromagnetic calorimeter and the missing mass is computed (the A' interacts weakly with Standard Model matter so it can't be detected). However, the A' mass range that can be explored with this technique is limited by the accessible energy in the center of mass frame, which goes as the square root of the beam energy. The realization of a 11 GeV positron beam at Jefferson Lab would allow to search for A' masses up to ˜ 100 MeV, reaching unexplored regions of the A' parameter space. A preliminary study on the feasibility of a PADME-like experiment at Jefferson Lab has been carried out, assuming a 11 GeV positron beam with a ˜ 100 nA current. The achievable sensitivity was estimated, studying the main sources of background (positron bremsstrahlung, annihilation into 2 gammas) using CALCHEP [3] and GEANT4 [4] simulations.

Computational imaging of light in flight

NASA Astrophysics Data System (ADS)

Hullin, Matthias B.

2014-10-01

Many computer vision tasks are hindered by image formation itself, a process that is governed by the so-called plenoptic integral. By averaging light falling into the lens over space, angle, wavelength and time, a great deal of information is irreversibly lost. The emerging idea of transient imaging operates on a time resolution fast enough to resolve non-stationary light distributions in real-world scenes. It enables the discrimination of light contributions by the optical path length from light source to receiver, a dimension unavailable in mainstream imaging to date. Until recently, such measurements used to require high-end optical equipment and could only be acquired under extremely restricted lab conditions. To address this challenge, we introduced a family of computational imaging techniques operating on standard time-of-flight image sensors, for the first time allowing the user to "film" light in flight in an affordable, practical and portable way. Just as impulse responses have proven a valuable tool in almost every branch of science and engineering, we expect light-in-flight analysis to impact a wide variety of applications in computer vision and beyond.

Frederick National Lab Collaboration Success Stories | FNLCR Staging

Cancer.gov

IBBR and Frederick National Lab Collaborate to Study Vaccine-Boosting Compounds The Frederick National Lab and the University of Maryland’s Institute for Bioscience and Biotechnology Research (IBBR) will work under a formal collaboration to eval

The interaction of light and gravity on the transmission of Echinostoma caproni (Digenea: Echinostomatidae) cercariae to the second intermediate host, Biomphalaria glabrata (Gastropoda: Pulmonata).

PubMed

Platt, Thomas R; Greenlee, Hali; Zelmer, Derek A

2010-04-01

The current experiments were designed to assess the interaction of light and gravity on the transmission of Echinostoma caproni cercariae to the second intermediate host, Biomphalaria glabrata. Transmission chambers were constructed of clear polyvinyl chloride pipe covered with a black sleeve to exclude light. Snails were constrained within the chamber to prevent movement, while permitting the cercariae to swim freely. A trial consisted of 2 infected B. glabrata shedding E. caproni cercariae placed at the center of the chamber with 5 uninfected B. glabrata placed 10 cm above and below the shedding snails as sentinels. Three experiments, consisting of 12 trials each, were conducted under the following lighting conditions, i.e., above and below the transmission chamber, and in complete darkness. In all 3 experiments, the proportion of metacercariae was significantly higher in snails at the top of the chamber. The results suggest that a negative geotaxis is the primary factor in the initial dispersal of E. caproni cercariae. Coupling negative geotaxis and positive phototaxis (light from above) resulted in a significantly higher proportion of metacercariae in sentinel snails at the top of the transmission chamber when corrected for cercarial density. There was no significant difference in the proportion of metacercariae in snails at the top or bottom of the transmission chamber with light at the bottom of the chamber or in complete darkness. Cercariae of E. caproni only respond to light in context, i.e., from above, and ignore the light stimulus when it comes from an unexpected location (bottom of the water column). Significantly greater numbers of cercariae were released from shedding snails when light was present, suggesting that emergence of cercariae from B. glabrata is dependent on light regardless of the position of the light source.

Microsoft Licenses Berkeley Lab's Home Energy Saver Code for Its Energy

Science.gov Websites

-based tool for calculating energy use in residential buildings. About one million people visit the Home Management Software | Berkeley Lab Berkeley Lab A-Z Index Directory Submit Web People Navigation Berkeley Lab Search Submit Web People Close About the Lab Leadership/Organization Calendar News

Reducing unnecessary lab testing in the ICU with artificial intelligence.

PubMed

Cismondi, F; Celi, L A; Fialho, A S; Vieira, S M; Reti, S R; Sousa, J M C; Finkelstein, S N

2013-05-01

To reduce unnecessary lab testing by predicting when a proposed future lab test is likely to contribute information gain and thereby influence clinical management in patients with gastrointestinal bleeding. Recent studies have demonstrated that frequent laboratory testing does not necessarily relate to better outcomes. Data preprocessing, feature selection, and classification were performed and an artificial intelligence tool, fuzzy modeling, was used to identify lab tests that do not contribute an information gain. There were 11 input variables in total. Ten of these were derived from bedside monitor trends heart rate, oxygen saturation, respiratory rate, temperature, blood pressure, and urine collections, as well as infusion products and transfusions. The final input variable was a previous value from one of the eight lab tests being predicted: calcium, PTT, hematocrit, fibrinogen, lactate, platelets, INR and hemoglobin. The outcome for each test was a binary framework defining whether a test result contributed information gain or not. Predictive modeling was applied to recognize unnecessary lab tests in a real world ICU database extract comprising 746 patients with gastrointestinal bleeding. Classification accuracy of necessary and unnecessary lab tests of greater than 80% was achieved for all eight lab tests. Sensitivity and specificity were satisfactory for all the outcomes. An average reduction of 50% of the lab tests was obtained. This is an improvement from previously reported similar studies with average performance 37% by [1-3]. Reducing frequent lab testing and the potential clinical and financial implications are an important issue in intensive care. In this work we present an artificial intelligence method to predict the benefit of proposed future laboratory tests. Using ICU data from 746 patients with gastrointestinal bleeding, and eleven measurements, we demonstrate high accuracy in predicting the likely information to be gained from proposed future

Reducing unnecessary lab testing in the ICU with artificial intelligence

PubMed Central

Cismondi, F.; Celi, L.A.; Fialho, A.S.; Vieira, S.M.; Reti, S.R.; Sousa, J.M.C.; Finkelstein, S.N.

2017-01-01

Objectives To reduce unnecessary lab testing by predicting when a proposed future lab test is likely to contribute information gain and thereby influence clinical management in patients with gastrointestinal bleeding. Recent studies have demonstrated that frequent laboratory testing does not necessarily relate to better outcomes. Design Data preprocessing, feature selection, and classification were performed and an artificial intelligence tool, fuzzy modeling, was used to identify lab tests that do not contribute an information gain. There were 11 input variables in total. Ten of these were derived from bedside monitor trends heart rate, oxygen saturation, respiratory rate, temperature, blood pressure, and urine collections, as well as infusion products and transfusions. The final input variable was a previous value from one of the eight lab tests being predicted: calcium, PTT, hematocrit, fibrinogen, lactate, platelets, INR and hemoglobin. The outcome for each test was a binary framework defining whether a test result contributed information gain or not. Patients Predictive modeling was applied to recognize unnecessary lab tests in a real world ICU database extract comprising 746 patients with gastrointestinal bleeding. Main results Classification accuracy of necessary and unnecessary lab tests of greater than 80% was achieved for all eight lab tests. Sensitivity and specificity were satisfactory for all the outcomes. An average reduction of 50% of the lab tests was obtained. This is an improvement from previously reported similar studies with average performance 37% by [1–3]. Conclusions Reducing frequent lab testing and the potential clinical and financial implications are an important issue in intensive care. In this work we present an artificial intelligence method to predict the benefit of proposed future laboratory tests. Using ICU data from 746 patients with gastrointestinal bleeding, and eleven measurements, we demonstrate high accuracy in predicting the

Latest results from FROST at Jefferson Lab

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ritchie, Barry G.

2014-06-01

The spectrum of broad and overlapping nucleon excitations can be greatly clarified by use of a polarized photon beam incident on a polarized target in meson photoproduction experiments. At Jefferson Lab, a program of such measurements has made use of the Jefferson Lab FROzen Spin Target (FROST). An overview of preliminary results are presented.

Toxoplasma gondii: A study of oocyst re-shedding in domestic cats.

PubMed

Zulpo, Dauton Luiz; Sammi, Ana Sue; Dos Santos, Joeleni Rosa; Sasse, João Pedro; Martins, Thais Agostinho; Minutti, Ana Flávia; Cardim, Sérgio Tosi; de Barros, Luiz Daniel; Navarro, Italmar Teodorico; Garcia, João Luis

2018-01-15

The aim of the present study was to evaluate the re-shedding of T. gondii oocysts in cats fed tissue cysts of homologous and heterologous strains 12, 24 and 36 months after the first infection. Thirteen cats were used in the present study and were divided into four groups: G1 (n=2), G2 (n=3), G3 (n=5), and G4 (n=3). G1, G3 and G4 cats were infected with brain cysts of ME49 and G2 with TgDoveBr8, both genotype II strains of T. gondii. The G1 and G2 cats were re-infected after twelve months with brain cysts of VEG strain (genotype III), and G3 cats were re-infected with TgDoveBr1 (genotype II). The G3 cats were re-infected a third time after 24 months from the second infection, and the G4 cats were re-infected 36 months after the initial infection with cysts of the VEG strain. The cats' feces were evaluated using fecal flotation and genotyped with PCR-RFLP. The serological responses for IgM, IgA and IgG were determined by ELISA. All cats shed oocysts after the initial infection. Only one G1 cat shed oocysts when re-infected after twelve months with the VEG strain. No G2 cats excreted oocysts after the second infection with VEG. G3 cats, when re-infected after twelve months with the TgDoveBr1 strain, did not shed oocysts. However, when challenged after a third time with the VEG strain, three out of four cats shed oocysts. In the G4 group, when re-infected after thirty-six months with the VEG strain, two out of three cats shed oocysts. All oocyst samples were genotyped and characterized as the same genotype from the inoculum. Protection against oocyst re-excretion occurred in 90%, 25%, and 33.4% of cats after 12, 24, and 36 months from the initial infection, respectively. Therefore, the environmental contamination by oocysts from re-infected adult cats is only 30% lower than from kittens. In conclusion, the excretion of T. gondii oocysts was higher in experimentally re-infected cats throughout the years, especially when a heterologous strain was used. Copyright © 2017

Syndecan-4 shedding impairs macrovascular angiogenesis in diabetes mellitus

DOE Office of Scientific and Technical Information (OSTI.GOV)

Li, Ran; Xie, Jun; Wu, Han

Purpose: Syndecan-4 (synd4) is a ubiquitous heparan sulfate proteoglycan cell surface receptor that modulates cell proliferation, migration, mechanotransduction, and endocytosis. The extracellular domain of synd4 sheds heavily in acute inflammation, but the shedding of synd4 in chronic inflammation, such as diabetes mellitus (DM), is still undefined. We investigated the alterations of synd4 endothelial expression in DM and the influence of impaired synd4 signaling on angiogenesis in human umbilical vein endothelial cells (HUVECs), diabetic rats, synd4 null mice, and db/db mice. Material and methods: HUVECs were incubated with advanced glycation end products (AGEs). Western blot analysis was used to determine synd4more » protein expression and ELISA was used to detect soluble synd4 fragments. The concentration of synd4 in the aortic endothelia of diabetic rats was detected by immunohistochemical staining. Aortic ring assays were performed to study the process of angiogenesis in the diabetic rats and in synd4 null and db/db mice. Recombinant adenoviruses containing the synd4 gene or null were constructed to enhance synd4 aortic expression in db/db mice. Results: Western blot analysis showed decreased expression of the synd4 extracellular domain in HUVECs, and ELISA detected increased soluble fragments of synd4 in the media. Synd4 endothelial expression in the aortas of diabetic rats was decreased. Aortic ring assay indicated impaired angiogenesis in synd4 null and db/db mice, which was partially reversed by synd4 overexpression in db/db mice. Conclusion: Synd4 shedding from vascular endothelial cells played an important role in the diabetes-related impairment of angiogenesis. -- Highlights: •Synd4 shedding from endothelial cells is accelerated under the stimulation of AGEs. •Extracellular domain of synd4 is diminished in the endothelium of DM rats. •Aortic rings of synd4 null mice showed impaired angiogenesis. •Overexpression of synd4 partly rescues

Kinetics of viral shedding provide insights into the epidemiology of viral hemorrhagic septicemia in Pacific herring

USGS Publications Warehouse

Hershberger, Paul K.; Gregg, Jacob L.; Winton, James R.; Grady, Courtney; Collins, Rachael

2010-01-01

Losses from infectious diseases are an important component of natural mortality among marine fish species, but factors controlling the ecology of these diseases and their potential responses to anthropogenic changes are poorly understood. We used viral hemorrhagic septicemia virus (VHSV) and a laboratory stock of Pacific herring Clupea pallasii to investigate the kinetics of viral shedding and its effect on disease transmission and host mortality. Outbreaks of acute disease, accompanied by mortality and viral shedding, were initiated after waterborne exposure of herring to concentrations of VHSV as low as 101 plaque-forming units (pfu) ml–1. Shed virus in flow-through tanks was first detected 4 to 5 d post-exposure, peaked after 6 to 10 d, and was no longer detected after 16 d. Shedding rates, calculated from density, flow and waterborne virus titer reached 1.8 to 5.0 × 108 pfu fish–1 d–1. Onset of viral shedding was dose-dependent and preceded initial mortality by 2 d. At 21 d, cumulative mortality in treatment groups ranged from 81 to 100% and was dependent not on challenge dose, but on the kinetics and level of viral shedding by infected fish in the tank. Possible consequences of the viral shedding and disease kinetics are discussed in the context of epizootic initiation and perpetuation among populations of wild Pacific herring.

Kinetics of viral shedding provide insights into the epidemiology of viral hemorrhagic septicemia in Pacific herring

USGS Publications Warehouse

Hershberger, P.; Gregg, J.; Grady, C.; Collins, R.; Winton, J.

2010-01-01

Losses from infectious diseases are an important component of natural mortality among marine fish species, but factors controlling the ecology of these diseases and their potential responses to anthropogenic changes are poorly understood. We used viral hemorrhagic septicemia virus (VHSV) and a laboratory stock of Pacific herring Clupea pallasii to investigate the kinetics of viral shedding and its effect on disease transmission and host mortality. Outbreaks of acute disease, accompanied by mortality and viral shedding, were initiated after waterborne exposure of herring to concentrations of VHSV as low as 10 1 plaque-forming units (pfu) ml-1. Shed virus in flow-through tanks was first detected 4 to 5 d post-exposure, peaked after 6 to 10 d, and was no longer detected after 16 d. Shedding rates, calculated from density, flow and waterborne virus titer reached 1.8 to 5.0 ?? ?10 8 pfu fish-1 d-1. Onset of viral shedding was dose-dependent and preceded initial mortality by 2 d. At 21 d, cumulative mortality in treatment groups ranged from 81 to 100% and was dependent not on challenge dose, but on the kinetics and level of viral shedding by infected fish in the tank. Possible consequences of the viral shedding and disease kinetics are discussed in the context of epizootic initiation and perpetuation among populations of wild Pacific herring. ?? Inter-Research 2010.

Emergence of Influenza A Virus Variants after Prolonged Shedding from Pheasants▿

PubMed Central

Humberd, Jennifer; Boyd, Kelli; Webster, Robert G.

2007-01-01

We previously demonstrated the susceptibility of pheasants to infection with influenza A viruses of 15 hemagglutinin (HA) subtypes: 13/23 viruses tested were isolated for ≥14 days, all in the presence of serum-neutralizing antibodies; one virus (H10) was shed for 45 days postinfection. Here we confirmed that 20% of pheasants shed low-pathogenic influenza viruses for prolonged periods. We aimed to determine why the antibody response did not clear the virus in the usual 3 to 10 days, because pheasants serve as a long-term source of influenza viruses in poultry markets. We found evidence of virus replication and histological changes in the large intestine, bursa of Fabricius, and cecal tonsil. The virus isolated 41 days postinfection was antigenically distinct from the parental H10 virus, with corresponding changes in the HA and neuraminidase. Ten amino acid differences were found between the parental H10 and the pheasant H10 virus; four were in potential antigenic sites of the HA molecule. Prolonged shedding of virus by pheasants results from a complex interplay between the diversity of virus variants and the host response. It is often argued that vaccination pressure is a mechanism that contributes to the generation of antigenic-drift variants in poultry. This study provided evidence that drift variants can occur naturally in pheasants after prolonged shedding of virus, thus strengthening our argument for the removal of pheasants from live-bird retail markets. PMID:17267493

The Mysterious Death: An HPLC Lab Experiment. An Undergraduate Forensic Lab

ERIC Educational Resources Information Center

Beussman, Douglas J.

2007-01-01

A high-performance liquid chromatography (HPLC) laboratory experiment based on the separation of four prescription drugs (disopyramide, lidocaine, procainamide, and quinidine) is presented. The experiment is set within the forensic science context of the discovery of a patient's mysterious death where a drug overdose is suspected. Each lab group…

Bovine coronavirus in naturally and experimentally exposed calves; viral shedding and the potential for transmission.

PubMed

Oma, Veslemøy Sunniva; Tråvén, Madeleine; Alenius, Stefan; Myrmel, Mette; Stokstad, Maria

2016-06-13

Bovine coronavirus (BCoV) is a widely distributed pathogen, causing disease and economic losses in the cattle industry worldwide. Prevention of virus spread is impeded by a lack of basic knowledge concerning viral shedding and transmission potential in individual animals. The aims of the study were to investigate the duration and quantity of BCoV shedding in feces and nasal secretions related to clinical signs, the presence of virus in blood and tissues and to test the hypothesis that seropositive calves are not infectious to naïve in-contact calves three weeks after BCoV infection. A live animal experiment was conducted, with direct contact between animal groups for 24 h as challenge procedure. Four naïve calves were commingled with a group of six naturally infected calves and sequentially euthanized. Two naïve sentinel calves were commingled with the experimentally exposed group three weeks after exposure. Nasal swabs, feces, blood and tissue samples were analyzed for viral RNA by RT-qPCR, and virus isolation was performed on nasal swabs. Serum was analyzed for BCoV antibodies. The calves showed mild general signs, and the most prominent signs were from the respiratory system. The overall clinical score corresponded well with the shedding of viral RNA the first three weeks after challenge. General depression and cough were the signs that correlated best with shedding of BCoV RNA, while peak respiratory rate and peak rectal temperature appeared more than a week later than the peak shedding. Nasal shedding preceded fecal shedding, and the calves had detectable amounts of viral RNA intermittently in feces through day 35 and in nasal secretions through day 28, however virus isolation was unsuccessful from day six and day 18 from the two calves investigated. Viral RNA was not detected in blood, but was found in lymphatic tissue through day 42 after challenge. Although the calves were shedding BCoV RNA 21 days after infection the sentinel animals were not infected

Sodium Blood Test: MedlinePlus Lab Test Information

MedlinePlus

... Lab Tests Online [Internet]. American Association for Clinical Chemistry; c2001–2017. Cirrhosis; [updated 2017 Jan 8; cited ... Lab Tests Online [Internet]. American Association for Clinical Chemistry; c2001–2017. Electrolytes: Common Questions [updated 2015 Dec ...

Evidence of Coat Color Variation Sheds New Light on Ancient Canids

PubMed Central

Ollivier, Morgane; Tresset, Anne; Hitte, Christophe; Petit, Coraline; Hughes, Sandrine; Gillet, Benjamin; Duffraisse, Marilyne; Pionnier-Capitan, Maud; Lagoutte, Laetitia; Arbogast, Rose-Marie; Balasescu, Adrian; Boroneant, Adina; Mashkour, Marjan; Vigne, Jean-Denis; Hänni, Catherine

2013-01-01

We have used a paleogenetics approach to investigate the genetic landscape of coat color variation in ancient Eurasian dog and wolf populations. We amplified DNA fragments of two genes controlling coat color, Mc1r (Melanocortin 1 Receptor) and CBD103 (canine-β-defensin), in respectively 15 and 19 ancient canids (dogs and wolf morphotypes) from 14 different archeological sites, throughout Asia and Europe spanning from ca. 12 000 B.P. (end of Upper Palaeolithic) to ca. 4000 B.P. (Bronze Age). We provide evidence of a new variant (R301C) of the Melanocortin 1 receptor (Mc1r) and highlight the presence of the beta-defensin melanistic mutation (CDB103-K locus) on ancient DNA from dog-and wolf-morphotype specimens. We show that the dominant KB allele (CBD103), which causes melanism, and R301C (Mc1r), the variant that may cause light hair color, are present as early as the beginning of the Holocene, over 10 000 years ago. These results underline the genetic diversity of prehistoric dogs. This diversity may have partly stemmed not only from the wolf gene pool captured by domestication but also from mutations very likely linked to the relaxation of natural selection pressure occurring in-line with this process. PMID:24098367

Evidence of coat color variation sheds new light on ancient canids.

PubMed

Ollivier, Morgane; Tresset, Anne; Hitte, Christophe; Petit, Coraline; Hughes, Sandrine; Gillet, Benjamin; Duffraisse, Marilyne; Pionnier-Capitan, Maud; Lagoutte, Laetitia; Arbogast, Rose-Marie; Balasescu, Adrian; Boroneant, Adina; Mashkour, Marjan; Vigne, Jean-Denis; Hänni, Catherine

2013-01-01

We have used a paleogenetics approach to investigate the genetic landscape of coat color variation in ancient Eurasian dog and wolf populations. We amplified DNA fragments of two genes controlling coat color, Mc1r (Melanocortin 1 Receptor) and CBD103 (canine-β-defensin), in respectively 15 and 19 ancient canids (dogs and wolf morphotypes) from 14 different archeological sites, throughout Asia and Europe spanning from ca. 12 000 B.P. (end of Upper Palaeolithic) to ca. 4000 B.P. (Bronze Age). We provide evidence of a new variant (R301C) of the Melanocortin 1 receptor (Mc1r) and highlight the presence of the beta-defensin melanistic mutation (CDB103-K locus) on ancient DNA from dog-and wolf-morphotype specimens. We show that the dominant K(B) allele (CBD103), which causes melanism, and R301C (Mc1r), the variant that may cause light hair color, are present as early as the beginning of the Holocene, over 10,000 years ago. These results underline the genetic diversity of prehistoric dogs. This diversity may have partly stemmed not only from the wolf gene pool captured by domestication but also from mutations very likely linked to the relaxation of natural selection pressure occurring in-line with this process.

Integrating Multiple On-line Knowledge Bases for Disease-Lab Test Relation Extraction.

PubMed

Zhang, Yaoyun; Soysal, Ergin; Moon, Sungrim; Wang, Jingqi; Tao, Cui; Xu, Hua

2015-01-01

A computable knowledge base containing relations between diseases and lab tests would be a great resource for many biomedical informatics applications. This paper describes our initial step towards establishing a comprehensive knowledge base of disease and lab tests relations utilizing three public on-line resources. LabTestsOnline, MedlinePlus and Wikipedia are integrated to create a freely available, computable disease-lab test knowledgebase. Disease and lab test concepts are identified using MetaMap and relations between diseases and lab tests are determined based on source-specific rules. Experimental results demonstrate a high precision for relation extraction, with Wikipedia achieving the highest precision of 87%. Combining the three sources reached a recall of 51.40%, when compared with a subset of disease-lab test relations extracted from a reference book. Moreover, we found additional disease-lab test relations from on-line resources, indicating they are complementary to existing reference books for building a comprehensive disease and lab test relation knowledge base.

LIVING LAB: User-Driven Innovation for Sustainability

ERIC Educational Resources Information Center

Liedtke, Christa; Welfens, Maria Jolanta; Rohn, Holger; Nordmann, Julia

2012-01-01

Purpose: The purpose of this paper is to summarize and discuss the results from the LIVING LAB design study, a project within the 7th Framework Programme of the European Union. The aim of this project was to develop the conceptual design of the LIVING LAB Research Infrastructure that will be used to research human interaction with, and stimulate…

NASA GeneLab Concept of Operations

NASA Technical Reports Server (NTRS)

Thompson, Terri; Gibbs, Kristina; Rask, Jon; Coughlan, Joseph; Smith, Jeffrey

2014-01-01

NASA's GeneLab aims to greatly increase the number of scientists that are using data from space biology investigations on board ISS, emphasizing a systems biology approach to the science. When completed, GeneLab will provide the integrated software and hardware infrastructure, analytical tools and reference datasets for an assortment of model organisms. GeneLab will also provide an environment for scientists to collaborate thereby increasing the possibility for data to be reused for future experimentation. To maximize the value of data from life science experiments performed in space and to make the most advantageous use of the remaining ISS research window, GeneLab will apply an open access approach to conducting spaceflight experiments by generating, and sharing the datasets derived from these biological studies in space.Onboard the ISS, a wide variety of model organisms will be studied and returned to Earth for analysis. Laboratories on the ground will analyze these samples and provide genomic, transcriptomic, metabolomic and proteomic data. Upon receipt, NASA will conduct data quality control tasks and format raw data returned from the omics centers into standardized, annotated information sets that can be readily searched and linked to spaceflight metadata. Once prepared, the biological datasets, as well as any analysis completed, will be made public through the GeneLab Space Bioinformatics System webb as edportal. These efforts will support a collaborative research environment for spaceflight studies that will closely resemble environments created by the Department of Energy (DOE), National Center for Biotechnology Information (NCBI), and other institutions in additional areas of study, such as cancer and environmental biology. The results will allow for comparative analyses that will help scientists around the world take a major leap forward in understanding the effect of microgravity, radiation, and other aspects of the space environment on model organisms

Baroclinic Instability and Energy Transfer underlying the Kuroshio eddy shedding process in Luzon Strait

NASA Astrophysics Data System (ADS)

Lu, J.

2016-02-01

The Kuroshio eddy shedding in Luzon Strait has been intensively studied, due to its important role in the energy budgets of the special gap-passing western boundary current and its potential influence to South China Sea. In this study, the eddy-mean flow interaction is first diagnosed with two classical "stationary" methods. Both show that, in a "time-averaged" sense, baroclinic instability and energy transfer provides the energy source for Kuroshio anticyclonic eddy shedding and the accompanied cyclonic eddy growth in Luzon Strait (this eddy pair will be called AC/C-Es for short). To take into account the "nonstationary and intermittent" nature, the temporal evolutions of energy transfer during a typical Kuroshio eddy shedding process are investigated using the localized multi-scale-window energy and vorticity analysis, or MS-EVA for short. Two stages are roughly distinguished according to the evolutionary nature of this process: the growing stage and the shedding stage. In the growing stage, the energy source straddles both the AC/C-Es, indicating mean flow supplies potential energy to both AC/C-Es for growth; the energy transfer hot spot persistently strengthens and expands horizontally as well as vertically from 200-300m to 100-400m depth range, culminating in a maximum of approximately 1.5×10-7 m2s-3. In the shedding stage, the energy source moves onto the accompanied cyclonic eddy, i.e., the mean flow now supplies energy mainly to the cyclonic eddy, making it strong enough to cut off the anticyclonic eddy from Kuroshio, leading to the Kuroshio eddy shedding.

Synchronized Schlieren method for vortex shedding in cascade during acoustic resonance

NASA Astrophysics Data System (ADS)

Nagashima, T.; Tanida, Y.

1986-10-01

An evaluation is made of synchronized schlieren optical system methods for the simultaneous visualization of both the acoustic wave and vortex shedding phenomena encountered during acoustic resonance excited by vortex shedding from the trailing edges of cascade blades. Attention is given to the case of parallel flat plate blades in throughflow velocities of up to 100 m/s. The acoustic wavefront is found to appear in the trailing edge region and travel upstream when a pair of vortices of opposite sign are fully developed at the trailing edge.

Coxiella burnetii shedding routes and antibody response after outbreaks of Q fever-induced abortion in dairy goat herds.

PubMed

Rousset, Elodie; Berri, Mustapha; Durand, Benoit; Dufour, Philippe; Prigent, Myriam; Delcroix, Thibault; Touratier, Anne; Rodolakis, Annie

2009-01-01

Q fever is a zoonosis caused by Coxiella burnetii, a bacterium largely carried by ruminants and shed into milk, vaginal mucus, and feces. The main potential hazard to humans and animals is due to shedding of bacteria that can then persist in the environment and be aerosolized. The purpose of this study was to evaluate shedding after an outbreak of Q fever abortion in goat herds and to assess the relationship with the occurrence of abortions and antibody responses. Aborting and nonaborting goats were monitored by PCR for C. burnetii shedding 15 and 30 days after the abortion episodes. PCR analysis of all samples showed that 70% (n = 50) of the aborting and 53% (n = 70) of the nonaborting goats were positive. C. burnetii was shed into vaginal mucus, feces, and milk of 44%, 21%, and 38%, respectively, of goats that aborted and 27%, 20%, and 31%, respectively, of goats that delivered normally. Statistical comparison of these shedding results did not reveal any difference between these two groups. PCR results obtained for the vaginal and fecal routes were concordant in 81% of cases, whereas those for milk correlated with only 49% of cases with either vaginal or fecal shedding status. Serological analysis, using enzyme-linked immunosorbent assay (ELISA), indirect immunofluorescence assay (IFA), and complement fixation tests, showed that at least 24% of the seronegative goats shed bacteria. Positive vaginal and fecal shedding, unlike positive milk shedding, was observed more often in animals that were weakly positive or negative by ELISA or IFA. Two opposite shedding trends were thus apparent for the milk and vaginal-fecal routes. Moreover, this study showed that a nonnegligible proportion of seronegative animals that delivered normally could excrete C. burnetii.

Group Size Effect on Cooperation in One-Shot Social Dilemmas II: Curvilinear Effect.

PubMed

Capraro, Valerio; Barcelo, Hélène

2015-01-01

In a world in which many pressing global issues require large scale cooperation, understanding the group size effect on cooperative behavior is a topic of central importance. Yet, the nature of this effect remains largely unknown, with lab experiments insisting that it is either positive or negative or null, and field experiments suggesting that it is instead curvilinear. Here we shed light on this apparent contradiction by considering a novel class of public goods games inspired to the realistic scenario in which the natural output limits of the public good imply that the benefit of cooperation increases fast for early contributions and then decelerates. We report on a large lab experiment providing evidence that, in this case, group size has a curvilinear effect on cooperation, according to which intermediate-size groups cooperate more than smaller groups and more than larger groups. In doing so, our findings help fill the gap between lab experiments and field experiments and suggest concrete ways to promote large scale cooperation among people.

Probing Cherenkov and Scintillation Light Separation for Next-Generation Neutrino Detectors

NASA Astrophysics Data System (ADS)

Caravaca, J.; Descamps, F. B.; Land, B. J.; Orebi Gann, G. D.; Wallig, J.; Yeh, M.

2017-09-01

The ability to separate Cherenkov and scintillation signals in liquid scintillator detectors would enable outstanding background rejection for next-generation neutrino experiments. Reconstruction of directional information, ring imaging, and sub-Cherenkov threshold detection all have the potential to substantially improve particle and event identification. The Cherenkov-Scintillation Separation (CHESS) experiment uses an array of small, fast photomultipliers (PMTs) and state-of-the-art electronics to demonstrate the reconstruction of a Cherenkov ring in a scintillation medium based on photon hit times and detected charge. This setup has been used to characterize the ability to detect Cherenkov light in a range of target media. We show results with pure organic scintillator (LAB) and the prospects with scintillators with a secondary fluor (LAB/PPO). There are future plans to deploy the newly developed water-based liquid scintillator, a medium with a higher Cherenkov/Scintillation light yield ratio than conventional pure liquid scintillators, enhancing the visibility of the less abundant Cherenkov light in the presence of scintillation light. These results can inform the development of future large-scale detectors, such as the proposed Theia experiment, or other large detectors at underground laboratories such as the far-site of the new Long Baseline Neutrino Facility at the Sanford Underground Research Facility. CHESS detector calibrations and commissioning will be discussed, and the latest results will be presented.

The experiment editor: supporting inquiry-based learning with virtual labs

NASA Astrophysics Data System (ADS)

Galan, D.; Heradio, R.; de la Torre, L.; Dormido, S.; Esquembre, F.

2017-05-01

Inquiry-based learning is a pedagogical approach where students are motivated to pose their own questions when facing problems or scenarios. In physics learning, students are turned into scientists who carry out experiments, collect and analyze data, formulate and evaluate hypotheses, and so on. Lab experimentation is essential for inquiry-based learning, yet there is a drawback with traditional hands-on labs in the high costs associated with equipment, space, and maintenance staff. Virtual laboratories are helpful to reduce these costs. This paper enriches the virtual lab ecosystem by providing an integrated environment to automate experimentation tasks. In particular, our environment supports: (i) scripting and running experiments on virtual labs, and (ii) collecting and analyzing data from the experiments. The current implementation of our environment supports virtual labs created with the authoring tool Easy Java/Javascript Simulations. Since there are public repositories with hundreds of freely available labs created with this tool, the potential applicability to our environment is considerable.

Electron Microscopy Lab

Science.gov Websites

Facilities Science Pillars Research Library Science Briefs Science News Science Highlights Lab Organizations Science Programs Applied Energy Programs Civilian Nuclear Energy Programs Laboratory Directed Research Science Seaborg Institute Fellows Conferences Research Opportunities Center for Integrated

Implementation of biological tissue Mueller matrix for polarization-sensitive optical coherence tomography based on LabVIEW

NASA Astrophysics Data System (ADS)

Lin, Yongping; Zhang, Xiyang; He, Youwu; Cai, Jianyong; Li, Hui

2018-02-01

The Jones matrix and the Mueller matrix are main tools to study polarization devices. The Mueller matrix can also be used for biological tissue research to get complete tissue properties, while the commercial optical coherence tomography system does not give relevant analysis function. Based on the LabVIEW, a near real time display method of Mueller matrix image of biological tissue is developed and it gives the corresponding phase retardant image simultaneously. A quarter-wave plate was placed at 45 in the sample arm. Experimental results of the two orthogonal channels show that the phase retardance based on incident light vector fixed mode and the Mueller matrix based on incident light vector dynamic mode can provide an effective analysis method of the existing system.

Highly efficient light management for perovskite solar cells

PubMed Central

Wang, Dong-Lin; Cui, Hui-Juan; Hou, Guo-Jiao; Zhu, Zhen-Gang; Yan, Qing-Bo; Su, Gang

2016-01-01

Organic-inorganic halide perovskite solar cells have enormous potential to impact the existing photovoltaic industry. As realizing a higher conversion efficiency of the solar cell is still the most crucial task, a great number of schemes were proposed to minimize the carrier loss by optimizing the electrical properties of the perovskite solar cells. Here, we focus on another significant aspect that is to minimize the light loss by optimizing the light management to gain a high efficiency for perovskite solar cells. In our scheme, the slotted and inverted prism structured SiO2 layers are adopted to trap more light into the solar cells, and a better transparent conducting oxide layer is employed to reduce the parasitic absorption. For such an implementation, the efficiency and the serviceable angle of the perovskite solar cell can be promoted impressively. This proposal would shed new light on developing the high-performance perovskite solar cells. PMID:26733112

A Case Study of a High School Fab Lab

NASA Astrophysics Data System (ADS)

Lacy, Jennifer E.

This dissertation examines making and design-based STEM education in a formal makerspace. It focuses on how the design and implementation of a Fab Lab learning environment and curriculum affect how instructors and students see themselves engaging in science, and how the Fab Lab relates to the social sorting practices that already take place at North High School. While there is research examining design-based STEM education in informal and formal learning environments, we know little about how K-12 teachers define STEM in making activities when no university or museum partnership exists. This study sought to help fill this gap in the research literature. This case study of a formal makerspace followed instructors and students in one introductory Fab Lab course for one semester. Additional observations of an introductory woodworking course helped build the case and set it into the school context, and provided supplementary material to better understand the similarities and differences between the Fab Lab course and a more traditional design-based learning course. Using evidence from observational field notes, participant interviews, course materials, and student work, I found that the North Fab Lab relies on artifacts and rhetoric symbolic of science and STEM to set itself apart from other design-based courses at North High School. Secondly, the North Fab Lab instructors and students were unable to explain how what they were doing in the Fab Lab was science, and instead relied on vague and unsupported claims related to interdisciplinary STEM practices and dated descriptions of science. Lastly, the design and implementation of the Fab Lab learning environment and curriculum and its separation from North High School's low tech, design-based courses effectively reinforced social sorting practices and cultural assumptions about student work and intelligence.

Jefferson Lab Virtual Tour

ScienceCinema

None

2018-01-16

Take a virtual tour of the campus of Thomas Jefferson National Accelerator Facility. You can see inside our two accelerators, three experimental areas, accelerator component fabrication and testing areas, high-performance computing areas and laser labs.

The History of Science and Technology at Bell Labs

NASA Astrophysics Data System (ADS)

Bishop, David

2008-03-01

Over the last 80 years, Bell Labs has been one of the most scientifically and technologically productive research labs in the world. Inventions such as the transistor, laser, cell phone, solar cell, negative feedback amplifier, communications satellite and many others were made there. Scientific breakthroughs such as discovery of the Big Bang, the wave nature of the electron, electron localization and the fractional quantum hall effect were also made there making Bell Labs almost unique in terms of large impacts in both science and technology. In my talk, I will discuss the history of the lab, talk about the present and give some suggestions for how I see it evolving into the future.

Measurement of light yield dependence on electron energy for SNO+ scintillator

NASA Astrophysics Data System (ADS)

Wan Chan Tseung, Hok

2011-10-01

SNO+ is a multi-purpose neutrino experiment whose reach extends to the following areas of neutrino physics: neutrinoless double beta decay (with Nd-loaded scintillator), geo-neutrinos, reactor and low-energy solar neutrinos, as well as supernova neutrinos. It is a ~780-tonne liquid scintillator detector currently under construction at the SNOLAB facility in Sudbury, Ontario,Canada. The scintillator to be used in SNO+ is linear alkylbenzene (LAB) with ~2 g/L of PPO (2,5-diphenyloxazole). In this talk, we describe an experiment to test the linearity of the response of LAB-PPO with respect to electrons. We find that below ~0.4 MeV, the energy scale of LAB-PPO becomes non-linear. An explanation is given in terms of Cherenkov light absorption and re-emission by the scintillator. This research has been supported under DOE Grant No. DE-FG02-97ER41020.

Towards a Flexible Language Lab for Community Colleges.

ERIC Educational Resources Information Center

Conway, Diana

1992-01-01

Suggestions are offered for ways to modify a typical community college language laboratory to serve diverse student needs. The discussion is based on experiences of Anchorage Community College, which modeled its lab on a learning resources center rather than a traditional lab. (LB)

Inhibition of TGF-β Signaling in SHED Enhances Endothelial Differentiation.

PubMed

Xu, J G; Gong, T; Wang, Y Y; Zou, T; Heng, B C; Yang, Y Q; Zhang, C F

2018-02-01

Low efficiency of deriving endothelial cells (ECs) from adult stem cells hampers their utilization in tissue engineering studies. The purpose of this study was to investigate whether suppression of transforming growth factor beta (TGF-β) signaling could enhance the differentiation efficiency of dental pulp-derived stem cells into ECs. We initially used vascular endothelial growth factor A (VEGF-A) to stimulate 2 dental pulp-derived stem cells (dental pulp stem cells and stem cells from human exfoliated deciduous teeth [SHED]) and compared their differentiation capacity into ECs. We further evaluated whether the vascular endothelial growth factor receptor I (VEGF-RI)-specific ligand placental growth factor-1 (PlGF-1) could mediate endothelial differentiation. Finally, we investigated whether the TGF-β signaling inhibitor SB-431542 could enhance the inductive effect of VEGF-A on endothelial differentiation, as well as the underlying mechanisms involved. ECs differentiated from dental pulp-derived stem cells exhibited the typical phenotypes of primary ECs, with SHED possessing a higher endothelial differentiation potential than dental pulp stem cells. VEGFR1-specific ligand-PLGF exerted a negligible effect on SHED-ECs differentiation. Compared with VEGF-A alone, the combination of VEGF-A and SB-431542 significantly enhanced the endothelial differentiation of SHED. The presence of SB-431542 inhibited the phosphorylation of Suppressor of Mothers Against Decapentaplegic 2/3 (SMAD2/3), allowing for VEGF-A-dependent phosphorylation and upregulation of VEGFR2. Our results indicate that the combination of VEGF-A and SB-431542 could enhance the differentiation of dental pulp-derived stem cells into endothelial cells, and this process is mediated through enhancement of VEGF-A-VEGFR2 signaling and concomitant inhibition of TGF-β-SMAD2/3 signaling.

Introducing ADS Labs

NASA Astrophysics Data System (ADS)

Accomazzi, Alberto; Henneken, E.; Grant, C. S.; Kurtz, M. J.; Di Milia, G.; Luker, J.; Thompson, D. M.; Bohlen, E.; Murray, S. S.

2011-05-01

ADS Labs is a platform that ADS is introducing in order to test and receive feedback from the community on new technologies and prototype services. Currently, ADS Labs features a new interface for abstract searches, faceted filtering of results, visualization of co-authorship networks, article-level recommendations, and a full-text search service. The streamlined abstract search interface provides a simple, one-box search with options for ranking results based on a paper relevancy, freshness, number of citations, and downloads. In addition, it provides advanced rankings based on collaborative filtering techniques. The faceted filtering interface allows users to narrow search results based on a particular property or set of properties ("facets"), allowing users to manage large lists and explore the relationship between them. For any set or sub-set of records, the co-authorship network can be visualized in an interactive way, offering a view of the distribution of contributors and their inter-relationships. This provides an immediate way to detect groups and collaborations involved in a particular research field. For a majority of papers in Astronomy, our new interface will provide a list of related articles of potential interest. The recommendations are based on a number of factors, including text similarity, citations, and co-readership information. The new full-text search interface allows users to find all instances of particular words or phrases in the body of the articles in our full-text archive. This includes all of the scanned literature in ADS as well as a select portion of the current astronomical literature, including ApJ, ApJS, AJ, MNRAS, PASP, A&A, and soon additional content from Springer journals. Fulltext search results include a list of the matching papers as well as a list of "snippets" of text highlighting the context in which the search terms were found. ADS Labs is available at http://adslabs.org

Time Trials--An AP Physics Challenge Lab

ERIC Educational Resources Information Center

Jones, David

2009-01-01

I have come to the conclusion that for high school physics classroom and laboratory experiences, simpler is better! In this paper I describe a very simple and effective lab experience that my AP students have thoroughly enjoyed year after year. I call this lab exercise "Time Trials." The experiment is simple in design and it is a lot of fun for…

A proteorhodopsin-based biohybrid light-powering pH sensor.

PubMed

Rao, Siyuan; Guo, Zhibin; Liang, Dawei; Chen, Deliang; Wei, Yen; Xiang, Yan

2013-10-14

The biohybrid sensor is an emerging technique for multi-functional detection that utilizes the instinctive responses or interactions of biomolecules. We develop a biohybrid pH sensor by taking advantage of the pH-dependent photoelectric characteristics of proteorhodopsin (pR). The transient absorption kinetics study indicates that the photoelectric behavior of pR is attributed to the varying lifetime of the M intermediate at different environmental pH values. This pR-based biohybrid light-powering sensor with microfluidic design can achieve real-time pH detection with quick response and high sensitivity. The results of this work would shed light on pR and its potential applications.

Elemental Chem Lab

ERIC Educational Resources Information Center

Franco Mariscal, Antonio Joaquin

2008-01-01

This educative material uses the symbols of 45 elements to spell the names of 32 types of laboratory equipment usually found in chemical labs. This teaching material has been divided into three puzzles according to the type of the laboratory equipment: (i) glassware as reaction vessels or containers; (ii) glassware for measuring, addition or…

Lab with Dad

ERIC Educational Resources Information Center

Havers, Brenda; Delmotte, Karen

2012-01-01

Family science nights are fantastic, but planning one can be overwhelming, especially when one considers the already overloaded schedule of a classroom teacher. To overcome this challenge, the authors--colleagues with a mutual love of science--developed a much simpler annual event called "Lab With Dad." The purpose was for one target age group of…

My Brother’s Keeper National Lab Week

NASA Image and Video Library

2016-03-02

Students in the My Brother’s Keeper program get an inside look at NASA Kennedy Space Center’s iconic Vehicle Assembly Building from the transfer aisle. The Florida spaceport is one of six NASA centers that participated in My Brother’s Keeper National Lab Week. The event is a nationwide effort to bring youth from underrepresented communities into federal labs and centers for hands-on activities, tours and inspirational speakers. Sixty students from the nearby cities of Orlando and Sanford visited Kennedy, where they toured the Vehicle Assembly Building, the Space Station Processing Facility and the center’s innovative Swamp Works Labs. The students also had a chance to meet and ask questions of a panel of subject matter experts from across Kennedy.

Concrete airship sheds at Orly, France. Part I

NASA Technical Reports Server (NTRS)

FREYSSINET

1925-01-01

This report details the contest to design and build concrete airship hangers. The difficulty lies in the magnitude of the absolute dimensions. An airship shed must withstand two principal types of stresses: those resulting from its own weight and those due to the wind. This report discusses both problems in detail.

Shedding of Japanese Encephalitis Virus in Oral Fluid of Infected Swine.

PubMed

Lyons, Amy C; Huang, Yan-Jang S; Park, So Lee; Ayers, Victoria B; Hettenbach, Susan M; Higgs, Stephen; McVey, D Scott; Noronha, Leela; Hsu, Wei-Wen; Vanlandingham, Dana L

2018-05-09

Japanese encephalitis virus (JEV) is a zoonotic mosquito-borne flavivirus endemic in the Asia-Pacific region. Maintenance of JEV in nature involves enzootic transmission by competent Culex mosquitoes among susceptible avian and swine species. Historically, JEV has been regarded as one of the most important arthropod-borne viruses in Southeast Asia. Oronasal shedding of JEV from infected amplification hosts was not recognized until the recent discovery of vector-free transmission of JEV among domestic pigs. In this study, oral shedding of JEV was characterized in domestic pigs and miniature swine representing the feral phenotype. A rope-based sampling method followed by the detection of viral RNA using RT-qPCR allowed the collection and detection of JEV in oral fluid samples collected from intradermally challenged animals. The results suggest that the shedding of JEV in oral fluid can be readily detected by molecular diagnostic assays at the acute phase of infection. It also demonstrates the feasibility of this technique for the diagnosis and surveillance of JEV in swine species.

The Role of Shed PrPc in the Neuropathogenesis of HIV Infection.

PubMed

Megra, Bezawit W; Eugenin, Eliseo A; Berman, Joan W

2017-07-01

HIV-1 enters the CNS soon after peripheral infection and causes chronic neuroinflammation and neuronal damage that leads to cognitive impairment in 40-70% of HIV-infected people. The nonpathogenic cellular isoform of the human prion protein (PrP c ) is an adhesion molecule constitutively expressed in the CNS. Previously, our laboratory showed that shed PrP c (sPrP c ) is increased in the cerebrospinal fluid of HIV-infected people with cognitive deficits as compared with infected people with no impairment. In this article, we demonstrate that CCL2 and TNF-α, inflammatory mediators that are elevated in the CNS of HIV-infected people, increase shedding of PrP c from human astrocytes by increasing the active form of the metalloprotease ADAM10. We show that the consequence of this shedding can be the production of inflammatory mediators, because treatment of astrocytes with rPrP c increased secretion of CCL2, CXCL-12, and IL-8. Supernatants from rPrP c -treated astrocytes containing factors produced in response to this treatment, but not rPrP c by itself, cause increased chemotaxis of both uninfected and HIV-infected human monocytes, suggesting a role for sPrP c in monocyte recruitment into the brain. Furthermore, we examined whether PrP c participates in glutamate uptake and found that rPrP c decreased uptake of this metabolite in astrocytes, which could lead to neurotoxicity and neuronal loss. Collectively, our data characterize mediators involved in PrP c shedding and the effect of this sPrP c on monocyte chemotaxis and glutamate uptake from astrocytes. We propose that shedding of PrP c could be a potential target for therapeutics to limit the cognitive impairment characteristic of neuroAIDS. Copyright © 2017 by The American Association of Immunologists, Inc.

Constructing the Components of a Lab Report Using Peer Review

ERIC Educational Resources Information Center

Berry, David E.; Fawkes, Kelli L.

2010-01-01

A protocol that emphasizes lab report writing using a piecemeal approach coupled with peer review is described. As the lab course progresses, the focus of the report writing changes sequentially through the abstract and introduction, the discussion, and the procedure. Two styles of lab programs are presented. One style rotates the students through…

Can Graduate Teaching Assistants Teach Inquiry-Based Geology Labs Effectively?

ERIC Educational Resources Information Center

Ryker, Katherine; McConnell, David

2014-01-01

This study examines the implementation of teaching strategies by graduate teaching assistants (GTAs) in inquiry-based introductory geology labs at a large research university. We assess the degree of inquiry present in each Physical Geology lab and compare and contrast the instructional practices of new and experienced GTAs teaching these labs. We…

Open web system of Virtual labs for nuclear and applied physics

NASA Astrophysics Data System (ADS)

Saldikov, I. S.; Afanasyev, V. V.; Petrov, V. I.; Ternovykh, M. Yu

2017-01-01

An example of virtual lab work on unique experimental equipment is presented. The virtual lab work is software based on a model of real equipment. Virtual labs can be used for educational process in nuclear safety and analysis field. As an example it includes the virtual lab called “Experimental determination of the material parameter depending on the pitch of a uranium-water lattice”. This paper included general description of this lab. A description of a database on the support of laboratory work on unique experimental equipment which is included this work, its concept development are also presented.

Imaging galectin-3 dependent endocytosis with lattice light-sheet microscopy

NASA Astrophysics Data System (ADS)

Baek, Jongho; Lou, Jieqiong; Coelho, Simao; Lim, Yean Jin; Seidlitz, Silvia; Nicovich, Philip R.; Wunder, Christian; Johannes, Ludger; Gaus, Katharina

2017-04-01

Lattice light-sheet (LLS) microscopy provides ultrathin light sheets of a two-dimensional optical lattice that allows us imaging three-dimensional (3D) objects for hundreds of time points at sub-second intervals and at or below the diffraction limit. Galectin-3 (Gal3), a carbohydrate-binding protein, triggers glycosphingolipid (GSL)-dependent biogenesis of morphologically distinct endocytic vesicles that are cargo specific and clathrin independent. In this study, we apply LLS microscopy to study the dynamics of Gal3 dependent endocytosis in live T cells. This will allow us to observe Gal3-mediated endocytosis at high temporal and excellent 3D spatial resolution, which may shed light on our understanding of the mechanism and physiological function of Gal3-induced endocytosis.

Sneak Preview of April 25 Science at the Theater

ScienceCinema

Ho, Shirley

2017-12-12

Berkeley Lab astrophysicist Shirley Ho offers a sneak preview of the Science at the Theatre event at the Berkeley Repertory Theatre on April 25. Three Berkeley Lab cosmologists and Bay Area astronomer Andrew Fraiknoi will gather at the Berkeley Rep on Monday, April 25, from 7 to 9 p.m. to shed light on the mystery of the accelerating universe. Topics will include hunting down Type 1a supernovae, measuring the universe using baryon oscillation, and whether dark energy is the true driver of the universe. If you have questions for the scientists, post them below, send them to friendsofberkeleylab@lbl.gov, or catch us on facebook: http://www.facebook.com/video/video.php?v=10150215592292354&oid=593833429...Your question might be answered at the April 25 talk if there's time.

Shiga Toxin-Producing Escherichia coli O157 Shedding Dynamics in an Australian Beef Herd

PubMed Central

Ahlstrom, Christina; Muellner, Petra; Lammers, Geraldine; Jones, Meghan; Octavia, Sophie; Lan, Ruiting; Heller, Jane

2017-01-01

Shiga toxin-producing Escherichia coli (STEC) O157 is an important foodborne pathogen that can be transmitted to humans both directly and indirectly from the feces of beef cattle, its primary reservoir. Numerous studies have investigated the shedding dynamics of E. coli O157 by beef cattle; however, the spatiotemporal trends of shedding are still not well understood. Molecular tools can increase the resolution through the use of strain typing to explore transmission dynamics within and between herds and identify strain-specific characteristics that may influence pathogenicity and spread. Previously, the shedding dynamics and molecular diversity, through the use of multilocus variable number of tandem repeat analysis (MLVA) of STEC O157, were separately investigated in an Australian beef herd over a 9-month study period. Variation in shedding was observed over time, and 33 MLVA types were identified. The study presented here combines the two datasets previously published with an aim to clarify the relationship between epidemiological variables and strain types. Three major genetic clusters (GCs) were identified that were significantly associated with the location of the cattle in different paddocks. No significant association between GCs and individual cow was observed. Results from this molecular epidemiological study provide evidence for herd-level clonal replacement over time that may have been triggered by movement to a new paddock. In conclusion, this study has provided further insight into STEC O157 shedding dynamics and pathogen transmission. Knowledge gaps remain regarding the relationship of strain types and the shedding dynamics of STEC O157 by beef cattle that could be further clarified through the use of whole-genome sequencing. PMID:29230401

Vortex Shedding Characteristics of the Wake of a Thin Flat Plate with a Circular Trailing Edge

NASA Technical Reports Server (NTRS)

Rai, Man Mohan

2018-01-01

The near and very near wake of a thin flat plate with a circular trailing edge are investigated with direct numerical simulations (DNS). Data obtained for two different Reynolds numbers (based on plate thickness, D) are the main focus of this study. The separating boundary layers are turbulent in both cases. An earlier investigation of one of the cases (Case F) showed shed vortices in the wake that were about 1.0 D to 4.0 D in spanwise length. Considerable variation in both the strength and frequency of these shed vortices was observed. One objective of the present investigation is to determine the important contributors to this variability in strength and frequency of shed vortices and their finite spanwise extent. Analysis of the data shows that streamwise vortices in the separating boundary layer play an important role in strengthening/weakening of the shed vortices and that high/low-speed streaks in the boundary layer are important contributors to variability in shedding frequency. Both these features of the boundary layer contribute to the finite extent of the vortices in the spanwise direction. The second plate DNS (Case G, with 40 percent of the plate thickness of Case F) shows that while shedding intensity is weaker than obtained in Case F, many of the wake features are similar to that of Case F. This is important in understanding the path to the wake of the thin plate with a sharp trailing edge where shedding is absent. Here we also test the efficacy of a functional relationship between the shedding frequency and the Reynolds numbers based on the boundary layer momentum thickness (Re (sub theta) and D (Re (sub D)); data for developing this behavioral model is from Cases F & G and five earlier DNSs of the flat plate wake.

Porcine circovirus type 3 (PCV3) shedding in sow colostrum.

PubMed

Kedkovid, Roongtham; Woonwong, Yonlayong; Arunorat, Jirapat; Sirisereewan, Chaitawat; Sangpratum, Nattaphong; Kesdangsakonwut, Sawang; Tummaruk, Padet; Teankum, Komkrich; Assavacheep, Pornchalit; Jittimanee, Suphattra; Thanawongnuwech, Roongroje

2018-07-01

The major objective of this work was to investigate the shedding of porcine circovirus type 3 (PCV3) in sow colostrum. PCV3 titers in the serum and colostrum samples of 38 sows were determined using qPCR. Interestingly, this is the first report regarding the identification of PCV3 from the colostrum samples. In the studied farm, the prevalence of PCV3 in the colostrum samples was 44.74% (17/38). When sows were grouped based on the PCV3 titers in the serum into the "High-viremic", "Low-viremic" and "Non-viremic" sows, it was shown that the High-viremic sows showed significantly higher PCV3 colostrum prevalence (100%; 9/9) with the PCV3 titers ranging from 4.01 to 7.33 genomic copies/mL. The results indicated that PCV3 in the colostrum might be partly influenced by the viremic stage of the infection. However, the results also showed that approximately 41% of sows shedding PCV3 with low titers in the colostrum (7/17) were non-viremic sows. In conclusion, this study identified the presence of PCV3 in sow colostrum. Clinical impacts and mechanisms of colostrum shedding of PCV3 should be further investigated. Copyright © 2018 Elsevier B.V. All rights reserved.

Inside Linden Lab

ERIC Educational Resources Information Center

Atkinson, Tom

2008-01-01

In this article, the author provides an overview of Second Life[trademark], or simply SL, which was developed at Linden Lab, a San Francisco-based corporation. SL is an online society within a threee-dimensional virtual world entirely built and owned by its residents, where they can explore, build, socialize and participate in their own economy.…

Optics and Light Activities for Teachers of all Grade Levels from Easily Obtainable Supplies

NASA Astrophysics Data System (ADS)

Lindgren, Richard; Hendricks, Curtis; Lucatorto, Lynn; McNeilus, Thomas; Thornton, Stephen

2010-02-01

Several hands-on activities in light and optics covering selected topics will be discussed in the context of home labs and how such activities can be incorporated into a distance-learning or online web-based course utilizing the latest communication technologies and the Internet. The presentation will focus on activities that can be constructed from easy to obtain supplies as well as a commercially available kit that we are having made available. Activities for teachers at the elementary level will focus on understanding light rays, shadows, and reflection from plane surfaces; at the middle school level will focus on curved mirrors and lenses, dispersion, and drawing ray diagrams; at the high school level will focus on Snell's law, the lens equation, wave interference, polarization, Young's experiment, and diffraction. A distance-learning, web-based course based on these home labs will be described. )

Frequency lock-in and phase synchronization of vortex shedding behind circular cylinder due to surface waves

NASA Astrophysics Data System (ADS)

Gunnoo, Hans; Abcha, Nizar; Ezersky, Alexander

2016-02-01

The influence of harmonic surface wave on non-regular Karman Vortex Street is investigated. In our experiments, Karman Street arises behind a vertical circular cylinder in a water flow and harmonic surface waves propagating upstream. It is found that surface waves can modify regimes of shedding in Karman Street: frequency lock-in and synchronization of vortex shedding can arise. Intensive surface waves can excite symmetric vortex street instead of chess-like street, and completely suppress shedding behind the cylinder. It is shown experimentally that such effects occur if frequency of harmonic surface wave is approximately twice higher than the frequency of vortex shedding. Region of frequency lock-in is found on the plane amplitude-frequency of surface wave.

Super-shedding and the link between human infection and livestock carriage of Escherichia coli O157.

PubMed

Chase-Topping, Margo; Gally, David; Low, Chris; Matthews, Louise; Woolhouse, Mark

2008-12-01

Cattle that excrete more Escherichia coli O157 than others are known as super-shedders. Super-shedding has important consequences for the epidemiology of E. coli O157 in cattle--its main reservoir--and for the risk of human infection, particularly owing to environmental exposure. Ultimately, control measures targeted at super-shedders may prove to be highly effective. We currently have only a limited understanding of both the nature and the determinants of super-shedding. However, super-shedding has been observed to be associated with colonization at the terminal rectum and might also occur more often with certain pathogen phage types. More generally, epidemiological evidence suggests that super-shedding might be important in other bacterial and viral infections.

RSV-encoded NS2 promotes epithelial cell shedding and distal airway obstruction

PubMed Central

Liesman, Rachael M.; Buchholz, Ursula J.; Luongo, Cindy L.; Yang, Lijuan; Proia, Alan D.; DeVincenzo, John P.; Collins, Peter L.; Pickles, Raymond J.

2014-01-01

Respiratory syncytial virus (RSV) infection is the major cause of bronchiolitis in young children. The factors that contribute to the increased propensity of RSV-induced distal airway disease compared with other commonly encountered respiratory viruses remain unclear. Here, we identified the RSV-encoded nonstructural 2 (NS2) protein as a viral genetic determinant for initiating RSV-induced distal airway obstruction. Infection of human cartilaginous airway epithelium (HAE) and a hamster model of disease with recombinant respiratory viruses revealed that NS2 promotes shedding of infected epithelial cells, resulting in two consequences of virus infection. First, epithelial cell shedding accelerated the reduction of virus titers, presumably by clearing virus-infected cells from airway mucosa. Second, epithelial cells shedding into the narrow-diameter bronchiolar airway lumens resulted in rapid accumulation of detached, pleomorphic epithelial cells, leading to acute distal airway obstruction. Together, these data indicate that RSV infection of the airway epithelium, via the action of NS2, promotes epithelial cell shedding, which not only accelerates viral clearance but also contributes to acute obstruction of the distal airways. Our results identify RSV NS2 as a contributing factor for the enhanced propensity of RSV to cause severe airway disease in young children and suggest NS2 as a potential therapeutic target for reducing the severity of distal airway disease. PMID:24713657

Vortex Shedding in the Wake Induced by a Real Elephant Seal Whisker

NASA Astrophysics Data System (ADS)

Turk, Jodi; Omilion, Alexis; Zhang, Wei; Kim, Jeong-Jae; Kim, Jeong-Ju; Choi, Woo-Rak; Lee, Sang-Joon

2017-11-01

Biomimicry has been adopted to create innovative solutions in a vast range of applications. One such application is the design of seal-whisker-inspired flow sensors for autonomous underwater vehicles (AUVs). In dark, cramped, and unstable terrain AUVs are not able to maneuver using visual and sonar-based navigation. Hence, it is critical to use underwater flow sensors to accurately detect minute disturbances in the surroundings. Certain seal whiskers exhibit a unique undulating three-dimensional morphology that can reduce vortex induced vibrations (VIVs) if the major axis of the whisker cross-section is aligned to the inflow. This allows the seal to precisely track prey fish upstream using solely their whiskers. The current study aims to understand the effect of a real seal whisker's morphology on the vortex shedding behavior. Despite extensive studies of wake induced by scaled whisker-like models, the vortex shedding in the wake of a real seal whisker is not well understood. A series of experiments are conducted with a high-speed Particle Imaging Velocimetry (PIV) system in a water channel to examine the vortex shedding downstream from a smooth whisker and an undulating whisker at a Reynolds number of a few hundred. Results of the vortex shedding induced by real seal whiskers can provide insights on developing high-sensitivity underwater flow sensors for AUVs and other whisker-inspired structures.

New rain shed (Building No. 241) interior showing posts, braces, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

New rain shed (Building No. 241) interior showing posts, braces, and roof structure. - Hawaii Volcanoes National Park Water Collection System, Hawaii Volcanoes National Park, Volcano, Hawaii County, HI

Introductory labs; what they don't, should, and can teach (and why)

NASA Astrophysics Data System (ADS)

Wieman, Carl

2016-03-01

Introductory physics labs are widely used and expensive. They have a wide variety of potential learning goals, but these are seldom specified and less often measured if they are achieved. We cover three different research projects on introductory labs: 1) We have done cognitive task analyses of both experimental research in physics and instructional labs. The striking differences explain much of the unhappiness expressed by students with labs: 2) We have measured the effectiveness of two introductory physics lab courses specifically intended to teach the physics content covered in standard introductory courses on mechanics and E & M. As measured by course exams, the benefit is 0 +/-2% for both. 3) We show how it is possible to use lab courses to teach students to correctly evaluate physical models with uncertain data. Such quantitative critical thinking is an important skill that is not learned in typical lab courses, but is well learned by our modified lab instruction.

Matriptase shedding is closely coupled with matriptase zymogen activation and requires de novo proteolytic cleavage likely involving its own activity

PubMed Central

Barndt, Robert; Gu, Yayun; Chen, Chien-Yu; Tseng, I-Chu; Su, Sheng-Fang; Wang, Jehng-Kang; Johnson, Michael D.

2017-01-01

The type 2 transmembrane serine protease matriptase is involved in many pathophysiological processes probably via its enzymatic activity, which depends on the dynamic relationship between zymogen activation and protease inhibition. Matriptase shedding can prolong the life of enzymatically active matriptase and increase accessibility to substrates. We show here that matriptase shedding occurs via a de novo proteolytic cleavage at sites located between the SEA domain and the CUB domain. Point or combined mutations at the four positively charged amino acid residues in the region following the SEA domain allowed Arg-186 to be identified as the primary cleavage site responsible for matriptase shedding. Kinetic studies further demonstrate that matriptase shedding is temporally coupled with matriptase zymogen activation. The onset of matriptase shedding lags one minute behind matriptase zymogen activation. Studies with active site triad Ser-805 point mutated matriptase, which no longer undergoes zymogen activation or shedding, further suggests that matriptase shedding depends on matriptase zymogen activation, and that matriptase proteolytic activity may be involved in its own shedding. Our studies uncover an autonomous mechanism coupling matriptase zymogen activation, proteolytic activity, and shedding such that a proportion of newly generated active matriptase escapes HAI-1-mediated rapid inhibition by shedding into the extracellular milieu. PMID:28829816

[Light and hens as attraction factors of Nyssomyia whitmani in a rural area, Southern Brazil].

PubMed

Teodoro, Ueslei; Lonardoni, Maria Valdrinez Campana; Silveira, Thaís Gomes Verzignassi; Dias, Alessandra de Cassia; Abbas, Milia; Alberton, Dayane; Santos, Demilson Rodrigues dos

2007-06-01

To verify the influence of traps with electric light and hens as factors that attract sandflies and compare results between capture methods. The study was conducted in the Palmital Farm, Southern Brazil. Sandfly collections were conducted with Falcão traps and an electric aspirator, fortnightly, between 8 p.m. and 11 p.m. in the presence or absence of light and hens in peridomiciliary areas, from September 1998 to June 1999. A total of 43,767 specimens from eight species of sandflies were collected: Nyssomyia whitmani, N. neivai and Migonemyia migonei constituting 99.9% of the total collected, with predominance of N. whitmani. The number of this species collected inside the hen's shed in the presence of hens (21,045) was greater than in their absence (10,434). In the presence of hens, with distinct intensities of light, a larger number of N. whitmani samples were collected with 3W light. In the presence of hens and light (3W), the number of N. whitmani collected with the electric aspirator (5,141) was superior to that collected with the Falcão trap (1,675). In the absence of light, with or without the presence hens, there was no difference between the numbers of N. whitmani collected with the electric aspirator or the Falcão trap. Hens and electric light together attract more N. whitmani to peridomicilary areas. The number of N. whitmani collected with an electric aspirator inside a hen's shed with the presence of hens and light is greater than those collected with a Falcão trap in the same conditions.

Perspectives on super-shedding of Escherichia coli O157:H7 by cattle.

PubMed

Munns, Krysty D; Selinger, L Brent; Stanford, Kim; Guan, Leluo; Callaway, Todd R; McAllister, Tim A

2015-02-01

Escherichia coli O157:H7 is a foodborne pathogen that causes illness in humans worldwide. Cattle are the primary reservoir of this bacterium, with the concentration and frequency of E. coli O157:H7 shedding varying greatly among individuals. The term "super-shedder" has been applied to cattle that shed concentrations of E. coli O157:H7 ≥ 10⁴ colony-forming units/g feces. Super-shedders have been reported to have a substantial impact on the prevalence and transmission of E. coli O157:H7 in the environment. The specific factors responsible for super-shedding are unknown, but are presumably mediated by characteristics of the bacterium, animal host, and environment. Super-shedding is sporadic and inconsistent, suggesting that biofilms of E. coli O157:H7 colonizing the intestinal epithelium in cattle are intermittently released into feces. Phenotypic and genotypic differences have been noted in E. coli O157:H7 recovered from super-shedders as compared to low-shedding cattle, including differences in phage type (PT21/28), carbon utilization, degree of clonal relatedness, tir polymorphisms, and differences in the presence of stx2a and stx2c, as well as antiterminator Q gene alleles. There is also some evidence to support that the native fecal microbiome is distinct between super-shedders and low-shedders and that low-shedders have higher levels of lytic phage within feces. Consequently, conditions within the host may determine whether E. coli O157:H7 can proliferate sufficiently for the host to obtain super-shedding status. Targeting super-shedders for mitigation of E. coli O157:H7 has been proposed as a means of reducing the incidence and spread of this pathogen to the environment. If super-shedders could be easily identified, strategies such as bacteriophage therapy, probiotics, vaccination, or dietary inclusion of plant secondary compounds could be specifically targeted at this subpopulation. Evidence that super-shedder isolates share a commonality with isolates

Behind the Scenes at Berkeley Lab - The Mechanical Fabrication Facility

ScienceCinema

Wells, Russell; Chavez, Pete; Davis, Curtis; Bentley, Brian

2018-04-16

Part of the Behind the Scenes series at Berkeley Lab, this video highlights the lab's mechanical fabrication facility and its exceptional ability to produce unique tools essential to the lab's scientific mission. Through a combination of skilled craftsmanship and precision equipment, machinists and engineers work with scientists to create exactly what's needed - whether it's measured in microns or meters.

New Features in ADS Labs

NASA Astrophysics Data System (ADS)

Accomazzi, Alberto; Kurtz, M. J.; Henneken, E. A.; Grant, C. S.; Thompson, D.; Di Milia, G.; Luker, J.; Murray, S. S.

2013-01-01

The NASA Astrophysics Data System (ADS) has been working hard on updating its services and interfaces to better support our community's research needs. ADS Labs is a new interface built on the old tried-and-true ADS Abstract Databases, so all of ADS's content is available through it. In this presentation we highlight the new features that have been developed in ADS Labs over the last year: new recommendations, metrics, a citation tool and enhanced fulltext search. ADS Labs has long been providing article-level recommendations based on keyword similarity, co-readership and co-citation analysis of its corpus. We have now introduced personal recommendations, which provide a list of articles to be considered based on a individual user's readership history. A new metrics interface provides a summary of the basic impact indicators for a list of records. These include the total and normalized number of papers, citations, reads, and downloads. Also included are some of the popular indices such as the h, g and i10 index. The citation helper tool allows one to submit a set of records and obtain a list of top 10 papers which cite and/or are cited by papers in the original list (but which are not in it). The process closely resembles the network approach of establishing "friends of friends" via an analysis of the citation network. The full-text search service now covers more than 2.5 million documents, including all the major astronomy journals, as well as physics journals published by Springer, Elsevier, the American Physical Society, the American Geophysical Union, and all of the arXiv eprints. The full-text search interface interface allows users and librarians to dig deep and find words or phrases in the body of the indexed articles. ADS Labs is available at http://adslabs.org

LabVIEW Interface for PCI-SpaceWire Interface Card

NASA Technical Reports Server (NTRS)

Lux, James; Loya, Frank; Bachmann, Alex

2005-01-01

This software provides a LabView interface to the NT drivers for the PCISpaceWire card, which is a peripheral component interface (PCI) bus interface that conforms to the IEEE-1355/ SpaceWire standard. As SpaceWire grows in popularity, the ability to use SpaceWire links within LabVIEW will be important to electronic ground support equipment vendors. In addition, there is a need for a high-level LabVIEW interface to the low-level device- driver software supplied with the card. The LabVIEW virtual instrument (VI) provides graphical interfaces to support all (1) SpaceWire link functions, including message handling and routing; (2) monitoring as a passive tap using specialized hardware; and (3) low-level access to satellite mission-control subsystem functions. The software is supplied in a zip file that contains LabVIEW VI files, which provide various functions of the PCI-SpaceWire card, as well as higher-link-level functions. The VIs are suitably named according to the matching function names in the driver manual. A number of test programs also are provided to exercise various functions.

Practical Clinical Training in Skills Labs: Theory and Practice

PubMed Central

Bugaj, T. J.; Nikendei, C.

2016-01-01

Today, skills laboratories or “skills labs”, i.e. specific practical skill training facilities, are a firmly established part of medical education offering the possibility of training clinical procedures in a safe and fault-forging environment prior to real life application at bedside or in the operating room. Skills lab training follows a structured teaching concept, takes place under supervision and in consideration of methodological-didactic concepts, ideally creating an atmosphere that allows the repeated, anxiety- and risk-free practice of targeted skills. In this selective literature review, the first section is devoted to (I) the development and dissemination of the skills lab concept. There follows (II) an outline of the underlying idea and (III) an analysis of key efficacy factors. Thereafter, (IV) the training method’s effectiveness and transference are illuminated, before (V) the use of student tutors, in the sense of peer-assisted-learning, in skills labs is discussed separately. Finally, (VI) the efficiency of the skills lab concept is analyzed, followed by an outlook on future developments and trends in the field of skills lab training. PMID:27579363

Rooftop detail of new rain shed (Building No. 241) with ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Rooftop detail of new rain shed (Building No. 241) with flume and overhead pipeline. - Hawaii Volcanoes National Park Water Collection System, Hawaii Volcanoes National Park, Volcano, Hawaii County, HI

Detail of new rain shed (Building No. 241). Note pipeline ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Detail of new rain shed (Building No. 241). Note pipeline connection from collection trough. - Hawaii Volcanoes National Park Water Collection System, Hawaii Volcanoes National Park, Volcano, Hawaii County, HI

EarthLabs - Investigating Hurricanes: Earth's Meteorological Monsters

NASA Astrophysics Data System (ADS)

McDaris, J. R.; Dahlman, L.; Barstow, D.

2007-12-01

Earth science is one of the most important tools that the global community needs to address the pressing environmental, social, and economic issues of our time. While, at times considered a second-rate science at the high school level, it is currently undergoing a major revolution in the depth of content and pedagogical vitality. As part of this revolution, labs in Earth science courses need to shift their focus from cookbook-like activities with known outcomes to open-ended investigations that challenge students to think, explore and apply their learning. We need to establish a new model for Earth science as a rigorous lab science in policy, perception, and reality. As a concerted response to this need, five states, a coalition of scientists and educators, and an experienced curriculum team are creating a national model for a lab-based high school Earth science course named EarthLabs. This lab course will comply with the National Science Education Standards as well as the states' curriculum frameworks. The content will focus on Earth system science and environmental literacy. The lab experiences will feature a combination of field work, classroom experiments, and computer access to data and visualizations, and demonstrate the rigor and depth of a true lab course. The effort is being funded by NOAA's Environmental Literacy program. One of the prototype units of the course is Investigating Hurricanes. Hurricanes are phenomena which have tremendous impact on humanity and the resources we use. They are also the result of complex interacting Earth systems, making them perfect objects for rigorous investigation of many concepts commonly covered in Earth science courses, such as meteorology, climate, and global wind circulation. Students are able to use the same data sets, analysis tools, and research techniques that scientists employ in their research, yielding truly authentic learning opportunities. This month-long integrated unit uses hurricanes as the story line by

INL’s TAP Systems

DOE Office of Scientific and Technical Information (OSTI.GOV)

None

2017-06-22

The starting point for many consumer products is the industrial manufacture of platform chemicals. The recent boom in domestic shale gas production makes it possible to envision a new resource for chemical manufacturing. Catalysts are the accelerants behind most industrial chemical reactions. A sophisticated research technique using a Temporal Analysis of Products (or TAP) reactor can now help. By shedding light on a catalyst’s fundamental step-by-step process, a TAP reactor can help chemists and chemical engineers understand why a new catalyst works better in the lab than in the chemical plant.

Stream piracy in the Black Hills: A geomorphology lab exercise

USGS Publications Warehouse

Zaprowski, B.J.; Evenson, E.B.; Epstein, J.B.

2002-01-01

The Black Hills of South Dakota exhibits many fine examples of stream piracy that are very suitable for teaching geomorphology lab exercises. This lab goes beyond standard topographic map interpretation by using geologic maps, well logs, gravel provenance and other types of data to teach students about stream piracy. Using a step-by-step method in which the lab exercises ramp up in difficulty, students hone their skills in deductive reasoning and data assimilation. The first exercises deal with the identification of stream piracy at a variety of spatial scales and the lab culminates with an exercise on landscape evolution and drainage rearrangement.

Creative Science Teaching Labs: New Dimensions in CPD

ERIC Educational Resources Information Center

Chappell, Kerry; Craft, Anna

2009-01-01

This paper offers analysis and evaluation of "Creative Science Teaching (CST) Labs III", a unique and immersive approach to science teachers' continuing professional development (CPD) designed and run by a London-based organisation, Performing Arts Labs (PAL), involving specialists from the arts, science and technology as integral. Articulating…

Online Writing Labs as Sites for Community Engagement

ERIC Educational Resources Information Center

Wells, Jaclyn Michelle

2010-01-01

This dissertation investigates the Community Writing and Education Station (CWEST), a community engagement project that partners a community adult basic literacy program with a university writing lab. The author argues that the community and university partners, the Lafayette Adult Resource Academy (LARA) and the Purdue Writing Lab, offer positive…

Consequences of Spatial Antisymmetry on Light

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mascarenhas, Angelo; Fluegel, Brian

2016-12-21

Light propagation in two and three dimensional lattices for which the index of refraction exhibits spatial antisymmetry is investigated in the ray and photonic crystal regimes. In these regimes, all the two dimensional antisymmetry groups for which light fails to propagate are identified. In the ray-regime, it is observed that in tilings described by 7 of the 46 two dimensional antisymmetric groups, light is localized within a fundamental domain and does not propagate through the tiling, in contrast to the behavior in the other 39 groups. To understand the above phenomenon, a rule based on the number of anti-mirror planesmore » passing through a single Bravais lattice point is derived. In the wave regime for photonic crystals, it is observed that there are no propagating eigensolutions for the same 7 tilings as above, whereas propagating solutions and energy pass band dispersion curves can be obtained for the other 39 groups. The reasons underlying this peculiar behavior are analyzed using the topological approach for modeling flow in dynamical billiards to shed light on the applicability of Bloch's theorem for these periodic antisymmetric lattices.« less

Berkeley Lab's Saul Perlmutter wins Nobel Prize in Physics | Berkeley Lab

Science.gov Websites

astrophysics, dark energy, physics Connect twitter instagram LinkedIn facebook youtube This form needs Berkeley Lab's Saul Perlmutter wins Nobel Prize in Physics News Release Paul Preuss 510-486-6249 * October professor of physics at the University of California at Berkeley, has won the 2011 Nobel Prize in Physics

Rooftop view of old rain shed (Building No. 43), pipeline ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Rooftop view of old rain shed (Building No. 43), pipeline on trestle, and water tanks. - Hawaii Volcanoes National Park Water Collection System, Hawaii Volcanoes National Park, Volcano, Hawaii County, HI

Rooftop view of new rain shed (Building No. 241) showing ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Rooftop view of new rain shed (Building No. 241) showing collection gutter and overhead pipeline. - Hawaii Volcanoes National Park Water Collection System, Hawaii Volcanoes National Park, Volcano, Hawaii County, HI