A globally convergent Lagrange and barrier function iterative algorithm for the traveling salesman problem.

PubMed

Dang, C; Xu, L

2001-03-01

In this paper a globally convergent Lagrange and barrier function iterative algorithm is proposed for approximating a solution of the traveling salesman problem. The algorithm employs an entropy-type barrier function to deal with nonnegativity constraints and Lagrange multipliers to handle linear equality constraints, and attempts to produce a solution of high quality by generating a minimum point of a barrier problem for a sequence of descending values of the barrier parameter. For any given value of the barrier parameter, the algorithm searches for a minimum point of the barrier problem in a feasible descent direction, which has a desired property that the nonnegativity constraints are always satisfied automatically if the step length is a number between zero and one. At each iteration the feasible descent direction is found by updating Lagrange multipliers with a globally convergent iterative procedure. For any given value of the barrier parameter, the algorithm converges to a stationary point of the barrier problem without any condition on the objective function. Theoretical and numerical results show that the algorithm seems more effective and efficient than the softassign algorithm.

Remote sensing optical instrumentation for enhanced space weather monitoring from the L1 and L5 Lagrange points

NASA Astrophysics Data System (ADS)

Kraft, S.; Puschmann, K. G.; Luntama, J. P.

2017-09-01

As part of the Space Situational Awareness Programme (SSA), ESA has initiated the assessment of two missions currently foreseen to be implemented to enable enhanced space weather monitoring. These missions utilize the positioning of satellites at the Lagrangian L1 and L5 points. These Phase 0 or Pre-Phase A mission studies are about to be completed and will thereby have soon passed the Mission Definition Review. Phase A studies are planned to start in 2017. The space weather monitoring system currently considers four remote sensing optical instruments and several in-situ instruments to analyse the Sun and the solar wind conditions, in order to provide early warnings of increased solar activity and to identify and mitigate potential threats to society and ground, airborne and space based infrastructure. The suggested optical instruments take heritage from ESA and NASA science missions like SOHO, STEREO and Solar Orbiter, but the instruments are foreseen to be optimized for operational space weather monitoring purposes with high reliability and robustness demands. The instruments are required to provide high quality measurements particularly during severe space weather events. The program intends to utilize the results of the on-going ESA instrument prototyping and technology development activities, and to initiate pre-developments of the operational space weather instruments to ensure the required maturity before the mission implementation.

Effect of Finite Particle Size on Convergence of Point Particle Models in Euler-Lagrange Multiphase Dispersed Flow

NASA Astrophysics Data System (ADS)

Nili, Samaun; Park, Chanyoung; Haftka, Raphael T.; Kim, Nam H.; Balachandar, S.

2017-11-01

Point particle methods are extensively used in simulating Euler-Lagrange multiphase dispersed flow. When particles are much smaller than the Eulerian grid the point particle model is on firm theoretical ground. However, this standard approach of evaluating the gas-particle coupling at the particle center fails to converge as the Eulerian grid is reduced below particle size. We present an approach to model the interaction between particles and fluid for finite size particles that permits convergence. We use the generalized Faxen form to compute the force on a particle and compare the results against traditional point particle method. We apportion the different force components on the particle to fluid cells based on the fraction of particle volume or surface in the cell. The application is to a one-dimensional model of shock propagation through a particle-laden field at moderate volume fraction, where the convergence is achieved for a well-formulated force model and back coupling for finite size particles. Comparison with 3D direct fully resolved numerical simulations will be used to check if the approach also improves accuracy compared to the point particle model. Work supported by the U.S. Department of Energy, National Nuclear Security Administration, Advanced Simulation and Computing Program, as a Cooperative Agreement under the Predictive Science Academic Alliance Program, under Contract No. DE-NA0002378.

Revisiting the Tale of Hercules: How Stars Orbiting the Lagrange Points Visit the Sun

NASA Astrophysics Data System (ADS)

Pérez-Villegas, Angeles; Portail, Matthieu; Wegg, Christopher; Gerhard, Ortwin

2017-05-01

We propose a novel explanation for the Hercules stream consistent with recent measurements of the extent and pattern speed of the Galactic bar. We have adapted a made-to-measure dynamical model tailored for the Milky Way to investigate the kinematics of the solar neighborhood (SNd). The model matches the 3D density of the red clump giant stars (RCGs) in the bulge and bar as well as stellar kinematics in the inner Galaxy, with a pattern speed of 39 km s-1 kpc-1. Cross-matching this model with the Gaia DR1 TGAS data combined with RAVE and LAMOST radial velocities, we find that the model naturally predicts a bimodality in the U-V-velocity distribution for nearby stars which is in good agreement with the Hercules stream. In the model, the Hercules stream is made of stars orbiting the Lagrange points of the bar which move outward from the bar’s corotation radius to visit the SNd. While the model is not yet a quantitative fit of the velocity distribution, the new picture naturally predicts that the Hercules stream is more prominent inward from the Sun and nearly absent only a few 100 pc outward of the Sun, and plausibly explains that Hercules is prominent in old and metal-rich stars.

Variational Integrators for Interconnected Lagrange-Dirac Systems

NASA Astrophysics Data System (ADS)

Parks, Helen; Leok, Melvin

2017-10-01

Interconnected systems are an important class of mathematical models, as they allow for the construction of complex, hierarchical, multiphysics, and multiscale models by the interconnection of simpler subsystems. Lagrange-Dirac mechanical systems provide a broad category of mathematical models that are closed under interconnection, and in this paper, we develop a framework for the interconnection of discrete Lagrange-Dirac mechanical systems, with a view toward constructing geometric structure-preserving discretizations of interconnected systems. This work builds on previous work on the interconnection of continuous Lagrange-Dirac systems (Jacobs and Yoshimura in J Geom Mech 6(1):67-98, 2014) and discrete Dirac variational integrators (Leok and Ohsawa in Found Comput Math 11(5), 529-562, 2011). We test our results by simulating some of the continuous examples given in Jacobs and Yoshimura (2014).

Minimum Propellant Low-Thrust Maneuvers near the Libration Points

NASA Astrophysics Data System (ADS)

Marinescu, A.; Dumitrache, M.

The impulse technique certainly can bring the vehicle on orbits around the libration points or close to them. The question that aries is, by what means can the vehicle arrive in such cases at the libration points? A first investigation carried out in this paper can give an answer: the use of the technique of low-thrust, which, in addition, can bring the vehicle from the libration points near to or into orbits around these points. This aspect is considered in this present paper where for the applications we have considered the transfer for orbits of the equidistant point L4 and of the collinear point L2, from Earth-moon system. This transfer maneuver can be used to insertion one satellite on libration points orbits. In Earth- moon system the points L 4 and L 5 because an vehicle in on of the equidistant points in quite stable and remains in its vicinity of perturbed, have potential interest for the establishment of transporder satellite for interplanetary tracking. In contrast an vehicle in one of the collinear points is quite instable and it will oscillate along the Earth-moon-axis at increasing amplitude and gradually escape from the libration point. Let use assume that a space vehicle equipped with a low-thrust propulsion is near a libration point L. We consider the planar motion in the restricted frame of the three bodies in the rotating system L, where the Earth-moon distance D=l. The unit of time T is period of the moon's orbit divided by 2 and multiplied by the square root of the quantity one plus the moon/Earth mass ratio, and the unit of mass is the Earth's mass. With these predictions the motion equatios of the vehicle equiped with a low-thrust propulsion installation in the linear approximation near the libration point, have been established. The parameters of the motion at the beginning and the end of these maneuvers are known, the variational problem has been formulated as a Lagrange type problem with fixed extremities. On established the differential

16QAM Blind Equalization via Maximum Entropy Density Approximation Technique and Nonlinear Lagrange Multipliers

PubMed Central

Mauda, R.; Pinchas, M.

2014-01-01

Recently a new blind equalization method was proposed for the 16QAM constellation input inspired by the maximum entropy density approximation technique with improved equalization performance compared to the maximum entropy approach, Godard's algorithm, and others. In addition, an approximated expression for the minimum mean square error (MSE) was obtained. The idea was to find those Lagrange multipliers that bring the approximated MSE to minimum. Since the derivation of the obtained MSE with respect to the Lagrange multipliers leads to a nonlinear equation for the Lagrange multipliers, the part in the MSE expression that caused the nonlinearity in the equation for the Lagrange multipliers was ignored. Thus, the obtained Lagrange multipliers were not those Lagrange multipliers that bring the approximated MSE to minimum. In this paper, we derive a new set of Lagrange multipliers based on the nonlinear expression for the Lagrange multipliers obtained from minimizing the approximated MSE with respect to the Lagrange multipliers. Simulation results indicate that for the high signal to noise ratio (SNR) case, a faster convergence rate is obtained for a channel causing a high initial intersymbol interference (ISI) while the same equalization performance is obtained for an easy channel (initial ISI low). PMID:24723813

Projection-based stabilization of interface Lagrange multipliers in immersogeometric fluid-thin structure interaction analysis, with application to heart valve modeling.

PubMed

Kamensky, David; Evans, John A; Hsu, Ming-Chen; Bazilevs, Yuri

2017-11-01

This paper discusses a method of stabilizing Lagrange multiplier fields used to couple thin immersed shell structures and surrounding fluids. The method retains essential conservation properties by stabilizing only the portion of the constraint orthogonal to a coarse multiplier space. This stabilization can easily be applied within iterative methods or semi-implicit time integrators that avoid directly solving a saddle point problem for the Lagrange multiplier field. Heart valve simulations demonstrate applicability of the proposed method to 3D unsteady simulations. An appendix sketches the relation between the proposed method and a high-order-accurate approach for simpler model problems.

Water production in comets C/2011 L4 (PanSTARRS) and C/2012 F6 (Lemmon) from observations with Soho/Swan

DOE Office of Scientific and Technical Information (OSTI.GOV)

Combi, M. R.; Aptekar, G.; Bertaux, J.-L.

2014-06-01

Comets C/2011 L4 (PanSTARRS) and C/2012 F6 (Lemmon) were observed throughout their 2012-2013 apparitions with the Solar Wind Anisotropies (SWAN) all-sky hydrogen Lyα camera on board the Solar and Heliosphere Observatory (SOHO) satellite. SOHO has been in a halo orbit around the L1 Earth-Sun Lagrange point since early 1996 and has been observing the interplanetary medium and comets beginning with C/1996 B2 (Hyakutake). The global water production from these comets was determined from an analysis of the SWAN Lyα camera observations. Comet C/2011 L4 (PanSTARRS), which reached its perihelion distance of 0.302 AU on 2013 March 10.17, was observed onmore » 50 days between 2013 January 29 and April 30. Comet C/2012 F6 (Lemmon), which reached its perihelion distance of 0.731 AU on 2013 March 24.51, was observed on 109 days between 2012 November 29 and 2013 June 31. The maximum water production rates were ∼1 × 10{sup 30} molecules s{sup –1} for both comets. The activities of both comets were asymmetric about perihelion. C/2011 L4 (PanSTARRS) was more active before perihelion than after, but C/2012 F6 (Lemmon) was more active after perihelion than before.« less

LAGRANGE: LAser GRavitational-wave ANtenna in GEodetic Orbit

NASA Astrophysics Data System (ADS)

Buchman, S.; Conklin, J. W.; Balakrishnan, K.; Aguero, V.; Alfauwaz, A.; Aljadaan, A.; Almajed, M.; Altwaijry, H.; Saud, T. A.; Byer, R. L.; Bower, K.; Costello, B.; Cutler, G. D.; DeBra, D. B.; Faied, D. M.; Foster, C.; Genova, A. L.; Hanson, J.; Hooper, K.; Hultgren, E.; Klavins, A.; Lantz, B.; Lipa, J. A.; Palmer, A.; Plante, B.; Sanchez, H. S.; Saraf, S.; Schaechter, D.; Shu, K.; Smith, E.; Tenerelli, D.; Vanbezooijen, R.; Vasudevan, G.; Williams, S. D.; Worden, S. P.; Zhou, J.; Zoellner, A.

2013-01-01

We describe a new space gravitational wave observatory design called LAG-RANGE that maintains all important LISA science at about half the cost and with reduced technical risk. It consists of three drag-free spacecraft in a geocentric formation. Fixed antennas allow continuous contact with the Earth, solving the problem of communications bandwidth and latency. A 70 mm diameter sphere with a 35 mm gap to its enclosure serves as the single inertial reference per spacecraft, operating in “true” drag-free mode (no test mass forcing). Other advantages are: a simple caging design based on the DISCOS 1972 drag-free mission, an all optical read-out with pm fine and nm coarse sensors, and the extensive technology heritage from the Honeywell gyroscopes, and the DISCOS and Gravity Probe B drag-free sensors. An Interferometric Measurement System, designed with reflective optics and a highly stabilized frequency standard, performs the ranging between test masses and requires a single optical bench with one laser per spacecraft. Two 20 cm diameter telescopes per spacecraft, each with infield pointing, incorporate novel technology developed for advanced optical systems by Lockheed Martin, who also designed the spacecraft based on a multi-flight proven bus structure. Additional technological advancements include updated drag-free propulsion, thermal control, charge management systems, and materials. LAGRANGE subsystems are designed to be scalable and modular, making them interchangeable with those of LISA or other gravitational science missions. We plan to space qualify critical technologies on small and nano satellite flights, with the first launch (UV-LED Sat) in 2013.

Centrifuge Rotor Models: A Comparison of the Euler-Lagrange and the Bond Graph Modeling Approach

NASA Technical Reports Server (NTRS)

Granda, Jose J.; Ramakrishnan, Jayant; Nguyen, Louis H.

2006-01-01

A viewgraph presentation on centrifuge rotor models with a comparison using Euler-Lagrange and bond graph methods is shown. The topics include: 1) Objectives; 2) MOdeling Approach Comparisons; 3) Model Structures; and 4) Application.

Error estimates of Lagrange interpolation and orthonormal expansions for Freud weights

NASA Astrophysics Data System (ADS)

Kwon, K. H.; Lee, D. W.

2001-08-01

Let Sn[f] be the nth partial sum of the orthonormal polynomials expansion with respect to a Freud weight. Then we obtain sufficient conditions for the boundedness of Sn[f] and discuss the speed of the convergence of Sn[f] in weighted Lp space. We also find sufficient conditions for the boundedness of the Lagrange interpolation polynomial Ln[f], whose nodal points are the zeros of orthonormal polynomials with respect to a Freud weight. In particular, if W(x)=e-(1/2)x2 is the Hermite weight function, then we obtain sufficient conditions for the inequalities to hold:andwhere and k=0,1,2...,r.

Predicting the safe load on backpacker's arm using Lagrange multipliers method

NASA Astrophysics Data System (ADS)

Abdalla, Faisal Saleh; Rambely, Azmin Sham

2014-09-01

In this study, a technique has been suggested to reduce a backpack load by transmitting determined loads to the children arm. The purpose of this paper is to estimate school children arm muscles while load carriage as well as to determine the safe load can be carried at wrist while walking with backpack. A mathematical model, as three DOFs model, was investigated in the sagittal plane and Lagrange multipliers method (LMM) was utilized to minimize a quadratic objective function of muscle forces. The muscle forces were minimized with three different load conditions which are termed as 0-L=0 N, 1-L=21.95 N, and 2-L=43.9 N. The investigated muscles were estimated and compared to their maximum forces throughout the load conditions. Flexor and extensor muscles were estimated and the results showed that flexor muscles were active while extensor muscles showed inactivity. The estimated muscle forces were didn't exceed their maximum forces with 0-L and 1-L conditions whereas biceps and FCR muscles exceeded their maximum forces with 2-L condition. Consequently, 1-L condition is quiet safe to be carried by hand whereas 2-L condition is not. Thus to reduce the load in the backpack the transmitted load shouldn't exceed 1-L condition.

End of Life Disposal for Three Libration Point Missions through Manipulation of the Jacobi Constant and Zero Velocity Curves

NASA Technical Reports Server (NTRS)

Petersen, Jeremy; Brown, Jonathan

2015-01-01

Flight Dynamics Facility (FDF) located at NASA Goddard Space Flight Center (GSFC) provides the flight dynamics expertise for three Sun-Earth Moon L1 missions. Advanced Composition Explorer (ACE) launched August 1997 Solar and Heliospheric Observatory (SOHO) launched December 1995 Global Geospace Science WIND satellite launched November 1994 entered Lagrange point orbit in 2004.

Optimal Sunshade Configurations for Space-Based Geoengineering near the Sun-Earth L1 Point.

PubMed

Sánchez, Joan-Pau; McInnes, Colin R

2015-01-01

Within the context of anthropogenic climate change, but also considering the Earth's natural climate variability, this paper explores the speculative possibility of large-scale active control of the Earth's radiative forcing. In particular, the paper revisits the concept of deploying a large sunshade or occulting disk at a static position near the Sun-Earth L1 Lagrange equilibrium point. Among the solar radiation management methods that have been proposed thus far, space-based concepts are generally seen as the least timely, albeit also as one of the most efficient. Large occulting structures could potentially offset all of the global mean temperature increase due to greenhouse gas emissions. This paper investigates optimal configurations of orbiting occulting disks that not only offset a global temperature increase, but also mitigate regional differences such as latitudinal and seasonal difference of monthly mean temperature. A globally resolved energy balance model is used to provide insights into the coupling between the motion of the occulting disks and the Earth's climate. This allows us to revise previous studies, but also, for the first time, to search for families of orbits that improve the efficiency of occulting disks at offsetting climate change on both global and regional scales. Although natural orbits exist near the L1 equilibrium point, their period does not match that required for geoengineering purposes, thus forced orbits were designed that require small changes to the disk attitude in order to control its motion. Finally, configurations of two occulting disks are presented which provide the same shading area as previously published studies, but achieve reductions of residual latitudinal and seasonal temperature changes.

Optimal Sunshade Configurations for Space-Based Geoengineering near the Sun-Earth L1 Point

PubMed Central

Sánchez, Joan-Pau; McInnes, Colin R.

2015-01-01

Within the context of anthropogenic climate change, but also considering the Earth’s natural climate variability, this paper explores the speculative possibility of large-scale active control of the Earth’s radiative forcing. In particular, the paper revisits the concept of deploying a large sunshade or occulting disk at a static position near the Sun-Earth L1 Lagrange equilibrium point. Among the solar radiation management methods that have been proposed thus far, space-based concepts are generally seen as the least timely, albeit also as one of the most efficient. Large occulting structures could potentially offset all of the global mean temperature increase due to greenhouse gas emissions. This paper investigates optimal configurations of orbiting occulting disks that not only offset a global temperature increase, but also mitigate regional differences such as latitudinal and seasonal difference of monthly mean temperature. A globally resolved energy balance model is used to provide insights into the coupling between the motion of the occulting disks and the Earth’s climate. This allows us to revise previous studies, but also, for the first time, to search for families of orbits that improve the efficiency of occulting disks at offsetting climate change on both global and regional scales. Although natural orbits exist near the L1 equilibrium point, their period does not match that required for geoengineering purposes, thus forced orbits were designed that require small changes to the disk attitude in order to control its motion. Finally, configurations of two occulting disks are presented which provide the same shading area as previously published studies, but achieve reductions of residual latitudinal and seasonal temperature changes. PMID:26309047

Integración automatizada de las ecuaciones de Lagrange en el movimiento orbital.

NASA Astrophysics Data System (ADS)

Abad, A.; San Juan, J. F.

The new techniques of algebraic manipulation, especially the Poisson Series Processor, permit the analytical integration of the more and more complex problems of celestial mechanics. The authors are developing a new Poisson Series Processor, PSPC, and they use it to solve the Lagrange equation of the orbital motion. They integrate the Lagrange equation by using the stroboscopic method, and apply it to the main problem of the artificial satellite theory.

78 FR 21491 - DeltaPoint Capital IV, L.P., DeltaPoint Capital IV (New York), L.P.; Notice Seeking Exemption...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-04-10

... SMALL BUSINESS ADMINISTRATION [License No. 02/02-0662, 02/02-0661] DeltaPoint Capital IV, L.P., DeltaPoint Capital IV (New York), L.P.; Notice Seeking Exemption Under Section 312 of the Small Business Investment Act, Conflicts of Interest Notice is hereby given that DeltaPoint Capital IV, L.P. and DeltaPoint...

The Relation Among the Likelihood Ratio-, Wald-, and Lagrange Multiplier Tests and Their Applicability to Small Samples,

DTIC Science & Technology

1982-04-01

S. (1979), "Conflict Among Criteria for Testing Hypothesis: Extension and Comments," Econometrica, 47, 203-207 Breusch , T. S. and Pagan , A. R. (1980...Savin, N. E. (1977), "Conflict Among Criteria for Testing Hypothesis in the Multivariate Linear Regression Model," Econometrica, 45, 1263-1278 Breusch , T...VNCLASSIFIED RAND//-6756NL U l~ I- THE RELATION AMONG THE LIKELIHOOD RATIO-, WALD-, AND LAGRANGE MULTIPLIER TESTS AND THEIR APPLICABILITY TO SMALL SAMPLES

Lagrange multiplier for perishable inventory model considering warehouse capacity planning

NASA Astrophysics Data System (ADS)

Amran, Tiena Gustina; Fatima, Zenny

2017-06-01

This paper presented Lagrange Muktiplier approach for solving perishable raw material inventory planning considering warehouse capacity. A food company faced an issue of managing perishable raw materials and marinades which have limited shelf life. Another constraint to be considered was the capacity of the warehouse. Therefore, an inventory model considering shelf life and raw material warehouse capacity are needed in order to minimize the company's inventory cost. The inventory model implemented in this study was the adapted economic order quantity (EOQ) model which is optimized using Lagrange multiplier. The model and solution approach were applied to solve a case industry in a food manufacturer. The result showed that the total inventory cost decreased 2.42% after applying the proposed approach.

The Sun-Earth saddle point: characterization and opportunities to test general relativity

NASA Astrophysics Data System (ADS)

Topputo, Francesco; Dei Tos, Diogene A.; Rasotto, Mirco; Nakamiya, Masaki

2018-04-01

The saddle points are locations where the net gravitational accelerations balance. These regions are gathering more attention within the astrophysics community. Regions about the saddle points present clean, close-to-zero background acceleration environments where possible deviations from General Relativity can be tested and quantified. Their location suggests that flying through a saddle point can be accomplished by leveraging highly nonlinear orbits. In this paper, the geometrical and dynamical properties of the Sun-Earth saddle point are characterized. A systematic approach is devised to find ballistic orbits that experience one or multiple passages through this point. A parametric analysis is performed to consider spacecraft initially on L_{1,2} Lagrange point orbits. Sun-Earth saddle point ballistic fly-through trajectories are evaluated and classified for potential use. Results indicate an abundance of short-duration, regular solutions with a variety of characteristics.

DSCOVR_EPIC_L1A

Atmospheric Science Data Center

2018-05-07

... Level:  L1 Platform:  DEEP SPACE CLIMATE OBSERVATORY Instrument:  CCD IMAGER Spatial ... L1B LAGRANGE Legacy:  Retired data product , click here for a newer version. For more information or ...

DSCOVR_EPIC_L1B

Atmospheric Science Data Center

2018-05-05

... Level:  L1 Platform:  DEEP SPACE CLIMATE OBSERVATORY Instrument:  CCD IMAGER Spatial ... L1B LAGRANGE Legacy:  Retired data product , click here for a newer version. For more information or ...

Technique to eliminate computational instability in multibody simulations employing the Lagrange multiplier

NASA Technical Reports Server (NTRS)

Watts, G.

1992-01-01

A programming technique to eliminate computational instability in multibody simulations that use the Lagrange multiplier is presented. The computational instability occurs when the attached bodies drift apart and violate the constraints. The programming technique uses the constraint equation, instead of integration, to determine the coordinates that are not independent. Although the equations of motion are unchanged, a complete derivation of the incorporation of the Lagrange multiplier into the equation of motion for two bodies is presented. A listing of a digital computer program which uses the programming technique to eliminate computational instability is also presented. The computer program simulates a solid rocket booster and parachute connected by a frictionless swivel.

Lagrange constraint neural network for audio varying BSS

NASA Astrophysics Data System (ADS)

Szu, Harold H.; Hsu, Charles C.

2002-03-01

Lagrange Constraint Neural Network (LCNN) is a statistical-mechanical ab-initio model without assuming the artificial neural network (ANN) model at all but derived it from the first principle of Hamilton and Lagrange Methodology: H(S,A)= f(S)- (lambda) C(s,A(x,t)) that incorporates measurement constraint C(S,A(x,t))= (lambda) ([A]S-X)+((lambda) 0-1)((Sigma) isi -1) using the vector Lagrange multiplier-(lambda) and a- priori Shannon Entropy f(S) = -(Sigma) i si log si as the Contrast function of unknown number of independent sources si. Szu et al. have first solved in 1997 the general Blind Source Separation (BSS) problem for spatial-temporal varying mixing matrix for the real world remote sensing where a large pixel footprint implies the mixing matrix [A(x,t)] necessarily fill with diurnal and seasonal variations. Because the ground truth is difficult to be ascertained in the remote sensing, we have thus illustrated in this paper, each step of the LCNN algorithm for the simulated spatial-temporal varying BSS in speech, music audio mixing. We review and compare LCNN with other popular a-posteriori Maximum Entropy methodologies defined by ANN weight matrix-[W] sigmoid-(sigma) post processing H(Y=(sigma) ([W]X)) by Bell-Sejnowski, Amari and Oja (BSAO) called Independent Component Analysis (ICA). Both are mirror symmetric of the MaxEnt methodologies and work for a constant unknown mixing matrix [A], but the major difference is whether the ensemble average is taken at neighborhood pixel data X's in BASO or at the a priori sources S variables in LCNN that dictates which method works for spatial-temporal varying [A(x,t)] that would not allow the neighborhood pixel average. We expected the success of sharper de-mixing by the LCNN method in terms of a controlled ground truth experiment in the simulation of variant mixture of two music of similar Kurtosis (15 seconds composed of Saint-Saens Swan and Rachmaninov cello concerto).

Accuracy of Lagrange-sinc functions as a basis set for electronic structure calculations of atoms and molecules

DOE Office of Scientific and Technical Information (OSTI.GOV)

Choi, Sunghwan; Hong, Kwangwoo; Kim, Jaewook

2015-03-07

We developed a self-consistent field program based on Kohn-Sham density functional theory using Lagrange-sinc functions as a basis set and examined its numerical accuracy for atoms and molecules through comparison with the results of Gaussian basis sets. The result of the Kohn-Sham inversion formula from the Lagrange-sinc basis set manifests that the pseudopotential method is essential for cost-effective calculations. The Lagrange-sinc basis set shows faster convergence of the kinetic and correlation energies of benzene as its size increases than the finite difference method does, though both share the same uniform grid. Using a scaling factor smaller than or equal tomore » 0.226 bohr and pseudopotentials with nonlinear core correction, its accuracy for the atomization energies of the G2-1 set is comparable to all-electron complete basis set limits (mean absolute deviation ≤1 kcal/mol). The same basis set also shows small mean absolute deviations in the ionization energies, electron affinities, and static polarizabilities of atoms in the G2-1 set. In particular, the Lagrange-sinc basis set shows high accuracy with rapid convergence in describing density or orbital changes by an external electric field. Moreover, the Lagrange-sinc basis set can readily improve its accuracy toward a complete basis set limit by simply decreasing the scaling factor regardless of systems.« less

Stability at Potential Maxima: The L-4 and L-5 Points of the Restricted Three-Body Problem.

ERIC Educational Resources Information Center

Greenberg, Richard; Davis, Donald R.

1978-01-01

Describes a dynamical system which is stable at potential maxima. The maxima, called L-4 and L-5, are stable locations of the restricted three-body problem. Energy loss from the system will tend to drive it away from stability. (GA)

Visualizing and Understanding the Components of Lagrange and Newton Interpolation

ERIC Educational Resources Information Center

Yang, Yajun; Gordon, Sheldon P.

2016-01-01

This article takes a close look at Lagrange and Newton interpolation by graphically examining the component functions of each of these formulas. Although interpolation methods are often considered simply to be computational procedures, we demonstrate how the components of the polynomial terms in these formulas provide insight into where these…

Scale-Limited Lagrange Stability and Finite-Time Synchronization for Memristive Recurrent Neural Networks on Time Scales.

PubMed

Xiao, Qiang; Zeng, Zhigang

2017-10-01

The existed results of Lagrange stability and finite-time synchronization for memristive recurrent neural networks (MRNNs) are scale-free on time evolvement, and some restrictions appear naturally. In this paper, two novel scale-limited comparison principles are established by means of inequality techniques and induction principle on time scales. Then the results concerning Lagrange stability and global finite-time synchronization of MRNNs on time scales are obtained. Scaled-limited Lagrange stability criteria are derived, in detail, via nonsmooth analysis and theory of time scales. Moreover, novel criteria for achieving the global finite-time synchronization are acquired. In addition, the derived method can also be used to study global finite-time stabilization. The proposed results extend or improve the existed ones in the literatures. Two numerical examples are chosen to show the effectiveness of the obtained results.

Modified Lagrange invariants and their role in determining transverse and axial imaging resolutions of self-interference incoherent holographic systems.

PubMed

Rosen, Joseph; Kelner, Roy

2014-11-17

The Lagrange invariant is a well-known law for optical imaging systems formulated in the frame of ray optics. In this study, we reformulate this law in terms of wave optics and relate it to the resolution limits of various imaging systems. Furthermore, this modified Lagrange invariant is generalized for imaging along the z axis, resulting with the axial Lagrange invariant which can be used to analyze the axial resolution of various imaging systems. To demonstrate the effectiveness of the theory, analysis of the lateral and the axial imaging resolutions is provided for Fresnel incoherent correlation holography (FINCH) systems.

78 FR 21491 - DeltaPoint Capital IV, L.P., DeltaPoint Capital IV (New York), L.P.; Notice Seeking Exemption...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-04-10

... Small Business Investment Act of 1958, as amended (``the Act''), in connection with the financing of a... SMALL BUSINESS ADMINISTRATION [License No. 02/02-0662, 02/02-0661] DeltaPoint Capital IV, L.P., DeltaPoint Capital IV (New York), L.P.; Notice Seeking Exemption Under Section 312 of the Small Business...

CarbonTracker-Lagrange: A Framework for Greenhouse Gas Flux Estimation at Regional to Continental Scales

NASA Astrophysics Data System (ADS)

Andrews, A. E.

2016-12-01

CarbonTracker-Lagrange (CT-L) is a flexible modeling framework developed to take advantage of newly available atmospheric data for CO2 and other long-lived gases such as CH4 and N2O. The North American atmospheric CO2 measurement network has grown from three sites in 2004 to >100 sites in 2015. The US network includes tall tower, mountaintop, surface, and aircraft sites in the NOAA Global Greenhouse Gas Reference Network along with sites maintained by university, government and private sector researchers. The Canadian network is operated by Environment and Climate Change Canada. This unprecedented dataset can provide spatially and temporally resolved CO2 emissions and uptake flux estimates and quantitative information about drivers of variability, such as drought and temperature. CT-L is a platform for systematic comparison of data assimilation techniques and evaluation of assumed prior, model and observation errors. A novel feature of CT-L is the optimization of boundary values along with surface fluxes, leveraging vertically resolved data available from NOAA's aircraft sampling program. CT-L uses observation footprints (influence functions) from the Weather Research and Forecasting/Stochastic Time-Inverted Lagrangian Transport (WRF-STILT) modeling system to relate atmospheric measurements to upwind fluxes and boundary values. Footprints are pre-computed and the optimization algorithms are efficient, so many variants of the calculation can be performed. Fluxes are adjusted using Bayesian or Geostatistical methods to provide optimal agreement with observations. Satellite measurements of CO2 and CH4 from GOSAT are available starting in July 2009 and from OCO-2 since September 2014. With support from the NASA Carbon Monitoring System, we are developing flux estimation strategies that use remote sensing and in situ data together, including geostatistical inversions using satellite retrievals of solar-induced chlorophyll fluorescence. CT-L enables quantitative

Using Solar Radiation Pressure to Control L2 Orbits

NASA Technical Reports Server (NTRS)

Tene, Noam; Richon, Karen; Folta, David

1998-01-01

The main perturbations at the Sun-Earth Lagrange points L1 and L2 are from solar radiation pressure (SRP), the Moon and the planets. Traditional approaches to trajectory design for Lagrange-point orbits use maneuvers every few months to correct for these perturbations. The gravitational effects of the Moon and the planets are small and periodic. However, they cannot be neglected because small perturbations in the direction of the unstable eigenvector are enough to cause exponential growth within a few months. The main effect of a constant SRP is to shift the center of the orbit by a small distance. For spacecraft with large sun-shields like the Microwave Anisotropy Probe (MAP) and the Next Generation Space Telescope (NGST), the SRP effect is larger than all other perturbations and depends mostly on spacecraft attitude. Small variations in the spacecraft attitude are large enough to excite or control the exponential eigenvector. A closed-loop linear controller based on the SRP variations would eliminate one of the largest errors to the orbit and provide a continuous acceleration for use in controlling other disturbances. It is possible to design reference trajectories that account for the periodic lunar and planetary perturbations and still satisfy mission requirements. When such trajectories are used the acceleration required to control the unstable eigenvector is well within the capabilities of a continuous linear controller. Initial estimates show that by using attitude control it should be possible to minimize and even eliminate thruster maneuvers for station keeping.

L-fuzzy fixed points theorems for L-fuzzy mappings via βℱL-admissible pair.

PubMed

Rashid, Maliha; Azam, Akbar; Mehmood, Nayyar

2014-01-01

We define the concept of βℱL-admissible for a pair of L-fuzzy mappings and establish the existence of common L-fuzzy fixed point theorem. Our result generalizes some useful results in the literature. We provide an example to support our result.

Euler-Lagrange formulas for pseudo-Kähler manifolds

NASA Astrophysics Data System (ADS)

Park, JeongHyeong

2016-01-01

Let c be a characteristic form of degree k which is defined on a Kähler manifold of real dimension m > 2 k. Taking the inner product with the Kähler form Ωk gives a scalar invariant which can be considered as a generalized Lovelock functional. The associated Euler-Lagrange equations are a generalized Einstein-Gauss-Bonnet gravity theory; this theory restricts to the canonical formalism if c =c2 is the second Chern form. We extend previous work studying these equations from the Kähler to the pseudo-Kähler setting.

L-Fuzzy Fixed Points Theorems for L-Fuzzy Mappings via β ℱL-Admissible Pair

PubMed Central

Rashid, Maliha; Azam, Akbar

2014-01-01

We define the concept of β ℱL-admissible for a pair of L-fuzzy mappings and establish the existence of common L-fuzzy fixed point theorem. Our result generalizes some useful results in the literature. We provide an example to support our result. PMID:24688441

Augmented Lagrange Hopfield network for solving economic dispatch problem in competitive environment

NASA Astrophysics Data System (ADS)

Vo, Dieu Ngoc; Ongsakul, Weerakorn; Nguyen, Khai Phuc

2012-11-01

This paper proposes an augmented Lagrange Hopfield network (ALHN) for solving economic dispatch (ED) problem in the competitive environment. The proposed ALHN is a continuous Hopfield network with its energy function based on augmented Lagrange function for efficiently dealing with constrained optimization problems. The ALHN method can overcome the drawbacks of the conventional Hopfield network such as local optimum, long computational time, and linear constraints. The proposed method is used for solving the ED problem with two revenue models of revenue based on payment for power delivered and payment for reserve allocated. The proposed ALHN has been tested on two systems of 3 units and 10 units for the two considered revenue models. The obtained results from the proposed methods are compared to those from differential evolution (DE) and particle swarm optimization (PSO) methods. The result comparison has indicated that the proposed method is very efficient for solving the problem. Therefore, the proposed ALHN could be a favorable tool for ED problem in the competitive environment.

Necessary conditions for weighted mean convergence of Lagrange interpolation for exponential weights

NASA Astrophysics Data System (ADS)

Damelin, S. B.; Jung, H. S.; Kwon, K. H.

2001-07-01

Given a continuous real-valued function f which vanishes outside a fixed finite interval, we establish necessary conditions for weighted mean convergence of Lagrange interpolation for a general class of even weights w which are of exponential decay on the real line or at the endpoints of (-1,1).

Pointwise convergence of derivatives of Lagrange interpolation polynomials for exponential weights

NASA Astrophysics Data System (ADS)

Damelin, S. B.; Jung, H. S.

2005-01-01

For a general class of exponential weights on the line and on (-1,1), we study pointwise convergence of the derivatives of Lagrange interpolation. Our weights include even weights of smooth polynomial decay near +/-[infinity] (Freud weights), even weights of faster than smooth polynomial decay near +/-[infinity] (Erdos weights) and even weights which vanish strongly near +/-1, for example Pollaczek type weights.

[MPLW515L point mutation in patients with myeloproliferative disease].

PubMed

Xia, Jun; Xu, Wei; Zhang, Su-Jiang; Fan, Lei; Qiao, Chun; Li, Jian-Yong

2008-12-01

In order to investigate the frequency of MPLW515L and JAK2V617F point mutations of the patients with myeloproliferative disease (MPD) in Nanjing area, MPLW515L and JAK2V617F point mutations were simultaneously detected by alleles specific polymerase chain reaction (AS-PCR) and sequencing in 190 MPD patients. The results showed that MPLW515L point mutation was detected in 1 out of 102 essential thrombocythemia (ET) patients (1.0%) and was not detected in 32 polycythemia vera (PV) patients, 13 idiopathic myelofibrosis (IMF) patients, 43 chronic myelogenous leukemia (CML) patients. JAK2V617F point mutation was detected in 20 out of 32 PV patients (62.5%), 43 out of 102 ET patients (42.2%), 5 out of 13 IMF patients (38.5%), and was not detected in 43 CML patients. It is concluded that MPLW515L point mutation exists in ET patient, but is not found in PV, IMF and CML. JAK2V617F point mutation exists in PV, ET and IMF, but not in CML.

On the commutator of C^{\\infty}} -symmetries and the reduction of Euler-Lagrange equations

NASA Astrophysics Data System (ADS)

Ruiz, A.; Muriel, C.; Olver, P. J.

2018-04-01

A novel procedure to reduce by four the order of Euler-Lagrange equations associated to nth order variational problems involving single variable integrals is presented. In preparation, a new formula for the commutator of two \

A composite experimental dynamic substructuring method based on partitioned algorithms and localized Lagrange multipliers

NASA Astrophysics Data System (ADS)

Abbiati, Giuseppe; La Salandra, Vincenzo; Bursi, Oreste S.; Caracoglia, Luca

2018-02-01

Successful online hybrid (numerical/physical) dynamic substructuring simulations have shown their potential in enabling realistic dynamic analysis of almost any type of non-linear structural system (e.g., an as-built/isolated viaduct, a petrochemical piping system subjected to non-stationary seismic loading, etc.). Moreover, owing to faster and more accurate testing equipment, a number of different offline experimental substructuring methods, operating both in time (e.g. the impulse-based substructuring) and frequency domains (i.e. the Lagrange multiplier frequency-based substructuring), have been employed in mechanical engineering to examine dynamic substructure coupling. Numerous studies have dealt with the above-mentioned methods and with consequent uncertainty propagation issues, either associated with experimental errors or modelling assumptions. Nonetheless, a limited number of publications have systematically cross-examined the performance of the various Experimental Dynamic Substructuring (EDS) methods and the possibility of their exploitation in a complementary way to expedite a hybrid experiment/numerical simulation. From this perspective, this paper performs a comparative uncertainty propagation analysis of three EDS algorithms for coupling physical and numerical subdomains with a dual assembly approach based on localized Lagrange multipliers. The main results and comparisons are based on a series of Monte Carlo simulations carried out on a five-DoF linear/non-linear chain-like systems that include typical aleatoric uncertainties emerging from measurement errors and excitation loads. In addition, we propose a new Composite-EDS (C-EDS) method to fuse both online and offline algorithms into a unique simulator. Capitalizing from the results of a more complex case study composed of a coupled isolated tank-piping system, we provide a feasible way to employ the C-EDS method when nonlinearities and multi-point constraints are present in the emulated system.

Cloud Point and Liquid-Liquid Equilibrium Behavior of Thermosensitive Polymer L61 and Salt Aqueous Two-Phase System.

PubMed

Rao, Wenwei; Wang, Yun; Han, Juan; Wang, Lei; Chen, Tong; Liu, Yan; Ni, Liang

2015-06-25

The cloud point of thermosensitive triblock polymer L61, poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) (PEO-PPO-PEO), was determined in the presence of various electrolytes (K2HPO4, (NH4)3C6H5O7, and K3C6H5O7). The cloud point of L61 was lowered by the addition of electrolytes, and the cloud point of L61 decreased linearly with increasing electrolyte concentration. The efficacy of electrolytes on reducing cloud point followed the order: K3C6H5O7 > (NH4)3C6H5O7 > K2HPO4. With the increase in salt concentration, aqueous two-phase systems exhibited a phase inversion. In addition, increasing the temperature reduced the concentration of salt needed that could promote phase inversion. The phase diagrams and liquid-liquid equilibrium data of the L61-K2HPO4/(NH4)3C6H5O7/K3C6H5O7 aqueous two-phase systems (before the phase inversion but also after phase inversion) were determined at T = (25, 30, and 35) °C. Phase diagrams of aqueous two-phase systems were fitted to a four-parameter empirical nonlinear expression. Moreover, the slopes of the tie-lines and the area of two-phase region in the diagram have a tendency to rise with increasing temperature. The capacity of different salts to induce aqueous two-phase system formation was the same order as the ability of salts to reduce the cloud point.

Observing Coronal Mass Ejections from the Sun-Earth L5 Point

NASA Astrophysics Data System (ADS)

Gopalswamy, N.; Davila, J. M.; St Cyr, O. C.

2013-12-01

Coronal mass ejections (CMEs) are the most energetic phenomenon in the heliosphere and are known to be responsible for severe space weather. Most of the current knowledge on CMEs accumulated over the past few decades has been derived from observations made from the Sun-Earth line, which is not the ideal vantage point to observe Earth-affecting CMEs (Gopalswamy et al., 2011a,b). The STEREO mission viewed CMEs from points away from the Sun-Earth line and demonstrated the importance of such observations in understanding the three-dimensional structure of CMEs and their true kinematics. In this paper, we show that it is advantageous to observe CMEs from the Sun-Earth L5 point in studying CMEs that affect Earth. In particular, these observations are important in identifying that part of the CME that is likely to arrive at Earth. L5 observations are critical for several aspects of CME studies such as: (i) they can also provide near-Sun space speed of CMEs, which is an important input for modeling Earth-arriving CMEs, (ii) backside and frontside CMEs can be readily distinguished even without inner coronal imagers, and (iii) preceding CMEs in the path of Earth-affecting CMEs can be identified for a better estimate of the travel time, which may not be possible from the Sun-Earth line. We also discuss how the L5 vantage point compares with the Sun-Earth L4 point for observing Earth-affecting CMEs. References Gopalswamy, N., Davila, J. M., St. Cyr, O. C., Sittler, E. C., Auchère, F., Duvall, T. L., Hoeksema, J. T., Maksimovic, M., MacDowall, R. J., Szabo, A., Collier, M. R. (2011a), Earth-Affecting Solar Causes Observatory (EASCO): A potential International Living with a Star Mission from Sun-Earth L5 JASTP 73, 658-663, DOI: 10.1016/j.jastp.2011.01.013 Gopalswamy, N., Davila, J. M., Auchère, F., Schou, J., Korendyke, C. M. Shih, A., Johnston, J. C., MacDowall, R. J., Maksimovic, M., Sittler, E., et al. (2011b), Earth-Affecting Solar Causes Observatory (EASCO): a mission at

78 FR 32294 - DeltaPoint Capital IV, L.P., DeltaPoint Capital IV (New York), L.P., License No. 02/02-0662,02/02...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-05-29

... Small Business Investment Act of 1958, as amended (``the Act''), in connection with the financing of a... SMALL BUSINESS ADMINISTRATION DeltaPoint Capital IV, L.P., DeltaPoint Capital IV (New York), L.P., License No. 02/02-0662,02/02-0661; Notice Seeking Exemption Under Section 312 of the Small Business...

L1 libration point manned space habitat

NASA Technical Reports Server (NTRS)

Luttges, Marvin; Johnson, Steve; Banks, Gary; Johnson, Richard; Meyer, Christian; Pepin, Scott; Macelroy, Robert

1989-01-01

Second generation stations or Manned Space Habitats (MSHs) are discussed for an Earth-Moon libration point and in lunar orbit. The conceptual design of such a station is outlined. Systems and subsystems described reflect anticipation of moderate technology growth. The evolution of the L1 environments is discussed, several selected subsystems are outlined, and how the L1 MSH will complete some of its activities is described.

A Fluid Structure Algorithm with Lagrange Multipliers to Model Free Swimming

NASA Astrophysics Data System (ADS)

Sahin, Mehmet; Dilek, Ezgi

2017-11-01

A new monolithic approach is prosed to solve the fluid-structure interaction (FSI) problem with Lagrange multipliers in order to model free swimming/flying. In the present approach, the fluid domain is modeled by the incompressible Navier-Stokes equations and discretized using an Arbitrary Lagrangian-Eulerian (ALE) formulation based on the stable side-centered unstructured finite volume method. The solid domain is modeled by the constitutive laws for the nonlinear Saint Venant-Kirchhoff material and the classical Galerkin finite element method is used to discretize the governing equations in a Lagrangian frame. In order to impose the body motion/deformation, the distance between the constraint pair nodes is imposed using the Lagrange multipliers, which is independent from the frame of reference. The resulting algebraic linear equations are solved in a fully coupled manner using a dual approach (null space method). The present numerical algorithm is initially validated for the classical FSI benchmark problems and then applied to the free swimming of three linked ellipses. The authors are grateful for the use of the computing resources provided by the National Center for High Performance Computing (UYBHM) under Grant Number 10752009 and the computing facilities at TUBITAK-ULAKBIM, High Performance and Grid Computing Center.

PID position regulation in one-degree-of-freedom Euler-Lagrange systems actuated by a PMSM

NASA Astrophysics Data System (ADS)

Verastegui-Galván, J.; Hernández-Guzmán, V. M.; Orrante-Sakanassi, J.

2018-02-01

This paper is concerned with position regulation in one-degree-of-freedom Euler-Lagrange Systems. We consider that the mechanical subsystem is actuated by a permanent magnet synchronous motor (PMSM). Our proposal consists of a Proportional-Integral-Derivative (PID) controller for the mechanical subsystem and a slight variation of field oriented control for the PMSM. We take into account the motor electric dynamics during the stability analysis. We present, for the first time, a global asymptotic stability proof for such a control scheme without requiring the mechanical subsystem to naturally possess viscous friction. Finally, as a corollary of our main result we prove global asymptotic stability for output feedback PID regulation of one-degree-of-freedom Euler-Lagrange systems when generated torque is considered as the system input, i.e. when the electric dynamics of PMSM's is not taken into account.

Distributed Fault-Tolerant Control of Networked Uncertain Euler-Lagrange Systems Under Actuator Faults.

PubMed

Chen, Gang; Song, Yongduan; Lewis, Frank L

2016-05-03

This paper investigates the distributed fault-tolerant control problem of networked Euler-Lagrange systems with actuator and communication link faults. An adaptive fault-tolerant cooperative control scheme is proposed to achieve the coordinated tracking control of networked uncertain Lagrange systems on a general directed communication topology, which contains a spanning tree with the root node being the active target system. The proposed algorithm is capable of compensating for the actuator bias fault, the partial loss of effectiveness actuation fault, the communication link fault, the model uncertainty, and the external disturbance simultaneously. The control scheme does not use any fault detection and isolation mechanism to detect, separate, and identify the actuator faults online, which largely reduces the online computation and expedites the responsiveness of the controller. To validate the effectiveness of the proposed method, a test-bed of multiple robot-arm cooperative control system is developed for real-time verification. Experiments on the networked robot-arms are conduced and the results confirm the benefits and the effectiveness of the proposed distributed fault-tolerant control algorithms.

The Impact of Discontinuity Front Orientation on the Accuracy of L1 Space Weather Forecasting

NASA Astrophysics Data System (ADS)

Szabo, A.

2013-12-01

Current space weather forecasting from the Sun-Earth first Lagrange (L1) point assumes that all observed solar wind discontinuity fronts (interplanetary shocks, ICME boundaries) are perpendicular to the Sun-Earth line and are propagating radially out from eh Sun. In reality, these weather fronts can have significantly tilted orientation. Combined ACE, Wind and Soho observations allow the quantification of this effect. With the launch of the DSCOVR spacecraft in early 2015, dual real-time solar wind measurements will become available (at least at some time). Algorithms and their impact exploiting this unique scenario will be discussed.

DSCOVR/EPIC Images and Science: A New Way to View the Entire Sunlit Earth From A Sun-Earth Lagrange-1 Orbit

NASA Astrophysics Data System (ADS)

Herman, J. R.; Marshak, A.; Szabo, A.

2015-12-01

The DSCOVR mission was launched into a Sun-Earth Lagrange-1 orbit 1.5 million kilometers from earth in February 2015 onboard a SpaceX Falcon-9 rocket. The solar wind and earth science instruments were tested during the 4.5 month journey to L-1. The first data were obtained during the June-July commissioning phase, which included the first moderate resolution (10 km) color images of the entire sunlit earth, color images of the Moon, and scientific data from 10 narrow band filters (317.5, 325, 340, 388, 443, 551, 680, 687.75, 764, and 779.5 nm). Three of these filters were used to construct the color images (443, 551, 680 nm) based on the average eye response histogram of the sunlit earth. This talk will discuss some of the issues involved in deriving science quality data for global ozone, the aerosol index (dust, smoke, and volcanic ash), cloud amounts and reflectivity, and cloud height (measured from the O2 A- and B-bands). As with most new satellites, the science data are preliminary.

Earth to Moon Transfer: Direct vs Via Libration Points (L1, L2)

NASA Technical Reports Server (NTRS)

Condon, Gerald L.; Wilson, Samuel W.

2004-01-01

For some three decades, the Apollo-style mission has served as a proven baseline technique for transporting flight crews to the Moon and back with expendable hardware. This approach provides an optimal design for expeditionary missions, emphasizing operational flexibility in terms of safely returning the crew in the event of a hardware failure. However, its application is limited essentially to low-latitude lunar sites, and it leaves much to be desired as a model for exploratory and evolutionary programs that employ reusable space-based hardware. This study compares the performance requirements for a lunar orbit rendezvous mission type with one using the cislunar libration point (L1) as a stopover and staging point for access to arbitrary sites on the lunar surface. For selected constraints and mission objectives, it contrasts the relative uniformity of performance cost when the L1 staging point is used with the wide variation of cost for the Apollo-style lunar orbit rendezvous.

Direct observations of L-I-H and H-I-L transitions with the X-point reciprocating probe in ASDEX Upgrade

DOE Office of Scientific and Technical Information (OSTI.GOV)

Müller, S. H.; Conway, G. D.; Birkenmeier, G.

A reciprocating Langmuir probe was used to directly measure the behavior of turbulence and flows in the X-point region during transitions between low-(L) and high-confinement (H) mode in ASDEX Upgrade. The probe traverses the divertor horizontally in 140 ms, typically 2–5 cm below the X-point. Toroidal Mach number, density, floating potential (ϕ{sub f}), and electron temperature (T{sub e}) are measured. In the regime accessible to the probe (P{sub inj}<1.5 MW, line-integrated core density <4×10{sup 19} m{sup −2}), the L-H transition features an intermediate phase (I-phase), characterized by limit-cycle oscillations at 0.5–3 kHz [Conway et al., Phys. Rev. Lett. 106, 065001 (2011)]. The probe measurements revealmore » that this pulsing affects both the density and the toroidal Mach number. It is present in both the low-(LFS) and high-field sides (HFS) of the scrape-off layer, while high-amplitude broadband turbulence usually dominates the private-flux region. Profile comparisons between L-mode and I-phase show lower density in pulsing regions and small shifts in T{sub e}, directed oppositely on LFS and HFS, which are compensated by shifts in ϕ{sub f} to yield a surprisingly unchanged plasma potential profile. Directly observed L-I-phase transitions reveal that the onset of the pulsing is preceded by a fast 50% density drop in the HFS X-point region. Back transitions to L-mode occur essentially symmetrically, with the pulsing stopping first, followed by a fast recovery to L-mode density levels in the divertor.« less

Transfer to the Collinear Libration Point L3 in the Sun-Earth+Moon System

NASA Technical Reports Server (NTRS)

Hou, Xi-yun; Tang, Jing-shi; Liu, Lin

2007-01-01

The collinear libration point L3 of the sun-earth+moon system is an ideal place for some space missions. Although there has been a great amount of work concerning the applications of the other two collinear libration points L1 and L2, little work has been done about the point L3. In this paper, the dynamics of the libration points was briefly introduced first. Then a way to transfer the spacecraft to the collinear libration point L3 via the invariant manifolds of the other two collinear libration points was proposed. Theoretical works under the model of circular restricted three-body problem were done. For the sun-earth+moon system, this model is a good approximation. The results obtained are useful when a transfer trajectory under the real solar system is designed.

DSCOVR: A New Perspective for Earth Observations from Space. Synergism and Complementarity with Existing Platforms

NASA Astrophysics Data System (ADS)

Valero, F. P.

2011-12-01

The Sun-Earth Lagrange points L-1 and L-2 mark positions where the gravitational pull of the Earth and Sun precisely equals the centripetal force required to rotate with the Earth about the Sun with the same orbital period as the Earth. Therefore, a satellite maintained at one of these Lagrange points would keep the same relative position to the Sun and the Earth and be able to observe most points on the planet as the Earth rotates during the day. L-1 and L-2 are of particular interest because a satellite at either location can easily be maintained near the Sun-Earth line and views the entire daytime hemisphere from L-1 and the entire nighttime hemisphere from L-2. Since L-1 and L-2 are in the ecliptic plane, synoptic, high temporal-resolution observations would be obtained as every point on the planet, including both polar regions, transits from sunrise to sunset (L-1) or from sunset to sunrise (L-2). In summary, a pair of deep-space observatories, one at L-1 (daytime) and one at L-2 (nighttime), could acquire minute by minute climate quality data for essentially every point on Earth, all observations simultaneously for the whole planet. Such unique attributes are incorporated in the Deep Space Climate Observatory (DSCOVR) that will systematically observe climate drivers (radiation, aerosols, ozone, clouds, oxygen A-band) from L-1 in ways not possible but synergistically complementary with platforms in Low Earth Orbit (LEO) or Geostationary Earth Orbit (GEO). The combination of Solar Lagrange Points (located in the ecliptic plane) GEO (located in the equatorial plane) and LEO platforms would certainly provide a powerful observational tool as well as enriched data sets for Earth sciences. Such synergism is greatly enhanced when one considers the potential of utilizing LEO, GEO, and Lagrange point satellites as components of an integrated observational system. For example, satellites at L-1 and L-2 will view the Earth plus the Moon while simultaneously having in

Optimal trajectories from the Earth-Moon L1 and L3 points to deflect hazardous asteroids and comets.

PubMed

Maccone, Claudio

2004-05-01

Software code named asteroff was recently created by the author to simulate the deflection of hazardous asteroids off of their collision course with the Earth. This code was both copyrighted and patented to avoid unauthorized use of ideas that could possibly be vital to construct a planetary defense system in the vicinity of the Earth. Having so said, the basic ideas and equations underlying the asteroff simulation code are openly described in this paper. A system of two space bases housing missiles is proposed to achieve the planetary defense of the Earth against dangerous asteroids and comets, collectively called impactors herein. We show that the layout of the Earth-Moon system with the five relevant Lagrangian (or libration) points in space leads naturally to only one, unmistakable location of these two space bases within the sphere of influence of the Earth. These locations are at the two Lagrangian points L(1) (between the Earth and the Moon) and L(3) (in the direction opposite to the Moon from the Earth). We show that placing missile bases at L(1) and L(3) would enable those missiles to deflect the trajectory of impactors by hitting them orthogonally to their impact trajectory toward the Earth, so as to maximize their deflection. We show that confocal conics are the best class of trajectories fulfilling this orthogonal deflection requirement. One additional remark is that the theory developed in this paper is just a beginning for a wider set of future research. In fact, we only develop the Keplerian analytical theory for the optimal planetary defense achievable from the Earth-Moon Lagrangian points L(1) and L(3). Much more sophisticated analytical refinements would be needed to: (1) take into account many perturbation forces of all kinds acting on both the impactors and missiles shot from L(1) and L(3); (2) add more (non-optimal) trajectories of missiles shot from either the Lagrangian points L(4) and L(5) of the Earth-Moon System or from the surface of the

Report on 3 and 4-point correlation statistics in the COBE DMR anisotrophy maps

NASA Technical Reports Server (NTRS)

Hinshaw, Gary (Principal Investigator); Gorski, Krzystof M.; Banday, Anthony J.; Bennett, Charles L.

1996-01-01

As part of the work performed under NASA contract # NAS5-32648, we have computed the 3-point and 4-point correlation functions of the COBE-DNIR 2-year and 4-year anisotropy maps. The motivation for this study was to search for evidence of non-Gaussian statistical fluctuations in the temperature maps: skewness or asymmetry in the case of the 3-point function, kurtosis in the case of the 4-point function. Such behavior would have very significant implications for our understanding of the processes of galaxy formation, because our current models of galaxy formation predict that non-Gaussian features should not be present in the DMR maps. The results of our work showed that the 3-point correlation function is consistent with zero and that the 4-point function is not a very sensitive probe of non-Gaussian behavior in the COBE-DMR data. Our computation and analysis of 3-point correlations in the 2-year DMR maps was published in the Astrophysical Journal Letters, volume 446, page L67, 1995. Our computation and analysis of 3-point correlations in the 4-year DMR maps will be published, together with some additional tests, in the June 10, 1996 issue of the Astrophysical Journal Letters. Copies of both of these papers are attached as an appendix to this report.

Enantioselective synthesis of L-(-)-4- boronophenylalanine (L-BPA)

DOEpatents

Samsel, Edward G.

1992-01-01

A method of making substantially pure L-BPA is disclosed. The method includes the steps of reacting 4-bromobenzaldehyde with ethylene glycol to form 4-bromobenzaldehyde ethylene glycol acetal, sequentially reacting 4-bromobenzaldehyde ethyleneglycol acetal with Mg to produce the Grignard reagent and thereafter reacting with tributyl borate and then converting to an acid environment to form 4-boronobenzaldehyde, reacting 4-boronobenzaldehyde with diethanol amine to form 4-boronobenzaldehyde diethanolamine ester, condensing the 4-boronobenzaldehyde diethanolamine ester with 2-phenyl-2-oxazolin-5-one to form an azlactone, reacting the azlactone with an alkali metal hydroxide to form z-.alpha.-benzoylamino-4-boronocinnamic acid, asymmetrically hydrogenating the z-.alpha.-benzoylamino-4-boronocinnamic acid in the presence of a catalyst of a cheltate complex of rhodium (I) with chiral bisphosphines to form L-(+)-N-benzoyl-4-boronophenylalanine, and thereafter acidifying the L-(+)-N-benzoyl-4-boronophenylalanine in an organic medium to produce L-BPA.

Enantioselective synthesis of L-(-)-4- boronophenylalanine (L-BPA)

DOEpatents

Samsel, E.G.

1992-10-20

A method of making substantially pure L-BPA is disclosed. The method includes the steps of reacting 4-bromobenzaldehyde with ethylene glycol to form 4-bromobenzaldehyde ethylene glycol acetal, sequentially reacting 4-bromobenzaldehyde ethyleneglycol acetal with Mg to produce the Grignard reagent and thereafter reacting with tributyl borate and then converting to an acid environment to form 4-boronobenzaldehyde, reacting 4-boronobenzaldehyde with diethanol amine to form 4-boronobenzaldehyde diethanolamine ester, condensing the 4-boronobenzaldehyde diethanolamine ester with 2-phenyl-2-oxazolin-5-one to form an azlactone, reacting the azlactone with an alkali metal hydroxide to form z-[alpha]-benzoylamino-4boronocinnamic acid, asymmetrically hydrogenating the z-[alpha]-benzoylamino-4-boronocinnamic acid in the presence of a catalyst of a cheltate complex of rhodium (I) with chiral bisphosphines to form L-(+)-N-benzoyl-4-boronophenylalanine, and thereafter acidifying the L-(+)-N-benzoyl-4-boronophenylalanine in an organic medium to produce L-BPA. 3 figs.

Free-Lagrange methods for compressible hydrodynamics in two space dimensions

NASA Astrophysics Data System (ADS)

Crowley, W. E.

1985-03-01

Since 1970 a research and development program in Free-Lagrange methods has been active at Livermore. The initial steps were taken with incompressible flows for simplicity. Since then the effort has been concentrated on compressible flows with shocks in two space dimensions and time. In general, the line integral method has been used to evaluate derivatives and the artificial viscosity method has been used to deal with shocks. Basically, two Free-Lagrange formulations for compressible flows in two space dimensions and time have been tested and both will be described. In method one, all prognostic quantities were node centered and staggered in time. The artificial viscosity was zone centered. One mesh reconnection philosphy was that the mesh should be optimized so that nearest neighbors were connected together. Another was that vertex angles should tend toward equality. In method one, all mesh elements were triangles. In method two, both quadrilateral and triangular mesh elements are permitted. The mesh variables are staggered in space and time as suggested originally by Richtmyer and von Neumann. The mesh reconnection strategy is entirely different in method two. In contrast to the global strategy of nearest neighbors, we now have a more local strategy that reconnects in order to keep the integration time step above a user chosen threshold. An additional strategy reconnects in the vicinity of large relative fluid motions. Mesh reconnection consists of two parts: (1) the tools that permits nodes to be merged and quads to be split into triangles etc. and; (2) the strategy that dictates how and when to use the tools. Both tools and strategies change with time in a continuing effort to expand the capabilities of the method. New ideas are continually being tried and evaluated.

Modeling of Mixing Behavior in a Combined Blowing Steelmaking Converter with a Filter-Based Euler-Lagrange Model

NASA Astrophysics Data System (ADS)

Li, Mingming; Li, Lin; Li, Qiang; Zou, Zongshu

2018-05-01

A filter-based Euler-Lagrange multiphase flow model is used to study the mixing behavior in a combined blowing steelmaking converter. The Euler-based volume of fluid approach is employed to simulate the top blowing, while the Lagrange-based discrete phase model that embeds the local volume change of rising bubbles for the bottom blowing. A filter-based turbulence method based on the local meshing resolution is proposed aiming to improve the modeling of turbulent eddy viscosities. The model validity is verified through comparison with physical experiments in terms of mixing curves and mixing times. The effects of the bottom gas flow rate on bath flow and mixing behavior are investigated and the inherent reasons for the mixing result are clarified in terms of the characteristics of bottom-blowing plumes, the interaction between plumes and top-blowing jets, and the change of bath flow structure.

Parallel processing a three-dimensional free-lagrange code

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mandell, D.A.; Trease, H.E.

1989-01-01

A three-dimensional, time-dependent free-Lagrange hydrodynamics code has been multitasked and autotasked on a CRAY X-MP/416. The multitasking was done by using the Los Alamos Multitasking Control Library, which is a superset of the CRAY multitasking library. Autotasking is done by using constructs which are only comment cards if the source code is not run through a preprocessor. The three-dimensional algorithm has presented a number of problems that simpler algorithms, such as those for one-dimensional hydrodynamics, did not exhibit. Problems in converting the serial code, originally written for a CRAY-1, to a multitasking code are discussed. Autotasking of a rewritten versionmore » of the code is discussed. Timing results for subroutines and hot spots in the serial code are presented and suggestions for additional tools and debugging aids are given. Theoretical speedup results obtained from Amdahl's law and actual speedup results obtained on a dedicated machine are presented. Suggestions for designing large parallel codes are given.« less

Voidage correction algorithm for unresolved Euler-Lagrange simulations

NASA Astrophysics Data System (ADS)

Askarishahi, Maryam; Salehi, Mohammad-Sadegh; Radl, Stefan

2018-04-01

The effect of grid coarsening on the predicted total drag force and heat exchange rate in dense gas-particle flows is investigated using Euler-Lagrange (EL) approach. We demonstrate that grid coarsening may reduce the predicted total drag force and exchange rate. Surprisingly, exchange coefficients predicted by the EL approach deviate more significantly from the exact value compared to results of Euler-Euler (EE)-based calculations. The voidage gradient is identified as the root cause of this peculiar behavior. Consequently, we propose a correction algorithm based on a sigmoidal function to predict the voidage experienced by individual particles. Our correction algorithm can significantly improve the prediction of exchange coefficients in EL models, which is tested for simulations involving Euler grid cell sizes between 2d_p and 12d_p . It is most relevant in simulations of dense polydisperse particle suspensions featuring steep voidage profiles. For these suspensions, classical approaches may result in an error of the total exchange rate of up to 30%.

Automatic markerless registration of point clouds with semantic-keypoint-based 4-points congruent sets

NASA Astrophysics Data System (ADS)

Ge, Xuming

2017-08-01

The coarse registration of point clouds from urban building scenes has become a key topic in applications of terrestrial laser scanning technology. Sampling-based algorithms in the random sample consensus (RANSAC) model have emerged as mainstream solutions to address coarse registration problems. In this paper, we propose a novel combined solution to automatically align two markerless point clouds from building scenes. Firstly, the method segments non-ground points from ground points. Secondly, the proposed method detects feature points from each cross section and then obtains semantic keypoints by connecting feature points with specific rules. Finally, the detected semantic keypoints from two point clouds act as inputs to a modified 4PCS algorithm. Examples are presented and the results compared with those of K-4PCS to demonstrate the main contributions of the proposed method, which are the extension of the original 4PCS to handle heavy datasets and the use of semantic keypoints to improve K-4PCS in relation to registration accuracy and computational efficiency.

Lagrange formula for differential operators on a tree-graph and the resolvents of well-posed restrictions of operator

NASA Astrophysics Data System (ADS)

Koshkarbayev, Nurbol; Kanguzhin, Baltabek

2017-09-01

In this paper we study the question on the full description of well-posed restrictions of given maximal differential operator on a tree-graph. Lagrange formula for differential operator on a tree with Kirchhoff conditions at its internal vertices is presented.

Domain decomposition methods for nonconforming finite element spaces of Lagrange-type

NASA Technical Reports Server (NTRS)

Cowsar, Lawrence C.

1993-01-01

In this article, we consider the application of three popular domain decomposition methods to Lagrange-type nonconforming finite element discretizations of scalar, self-adjoint, second order elliptic equations. The additive Schwarz method of Dryja and Widlund, the vertex space method of Smith, and the balancing method of Mandel applied to nonconforming elements are shown to converge at a rate no worse than their applications to the standard conforming piecewise linear Galerkin discretization. Essentially, the theory for the nonconforming elements is inherited from the existing theory for the conforming elements with only modest modification by constructing an isomorphism between the nonconforming finite element space and a space of continuous piecewise linear functions.

Generalized Lagrange Jacobi Gauss-Lobatto (GLJGL) Collocation Method for Solving Linear and Nonlinear Fokker-Planck Equations

NASA Astrophysics Data System (ADS)

Parand, K.; Latifi, S.; Moayeri, M. M.; Delkhosh, M.

2018-05-01

In this study, we have constructed a new numerical approach for solving the time-dependent linear and nonlinear Fokker-Planck equations. In fact, we have discretized the time variable with Crank-Nicolson method and for the space variable, a numerical method based on Generalized Lagrange Jacobi Gauss-Lobatto (GLJGL) collocation method is applied. It leads to in solving the equation in a series of time steps and at each time step, the problem is reduced to a problem consisting of a system of algebraic equations that greatly simplifies the problem. One can observe that the proposed method is simple and accurate. Indeed, one of its merits is that it is derivative-free and by proposing a formula for derivative matrices, the difficulty aroused in calculation is overcome, along with that it does not need to calculate the General Lagrange basis and matrices; they have Kronecker property. Linear and nonlinear Fokker-Planck equations are given as examples and the results amply demonstrate that the presented method is very valid, effective, reliable and does not require any restrictive assumptions for nonlinear terms.

Prediction of a Densely Loaded Particle-Laden Jet using a Euler-Lagrange Dense Spray Model

NASA Astrophysics Data System (ADS)

Pakseresht, Pedram; Apte, Sourabh V.

2017-11-01

Modeling of a dense spray regime using an Euler-Lagrange discrete-element approach is challenging because of local high volume loading. A subgrid cluster of droplets can lead to locally high void fractions for the disperse phase. Under these conditions, spatio-temporal changes in the carrier phase volume fractions, which are commonly neglected in spray simulations in an Euler-Lagrange two-way coupling model, could become important. Accounting for the carrier phase volume fraction variations, leads to zero-Mach number, variable density governing equations. Using pressure-based solvers, this gives rise to a source term in the pressure Poisson equation and a non-divergence free velocity field. To test the validity and predictive capability of such an approach, a round jet laden with solid particles is investigated using Direct Numerical Simulation and compared with available experimental data for different loadings. Various volume fractions spanning from dilute to dense regimes are investigated with and without taking into account the volume displacement effects. The predictions of the two approaches are compared and analyzed to investigate the effectiveness of the dense spray model. Financial support was provided by National Aeronautics and Space Administration (NASA).

Synthesis of a controller for stabilizing the motion of a rigid body about a fixed point

NASA Astrophysics Data System (ADS)

Zabolotnov, Yu. M.; Lobanov, A. A.

2017-05-01

A method for the approximate design of an optimal controller for stabilizing the motion of a rigid body about a fixed point is considered. It is assumed that rigid body motion is nearly the motion in the classical Lagrange case. The method is based on the common use of the Bellman dynamic programming principle and the averagingmethod. The latter is used to solve theHamilton-Jacobi-Bellman equation approximately, which permits synthesizing the controller. The proposed method for controller design can be used in many problems close to the problem of motion of the Lagrange top (the motion of a rigid body in the atmosphere, the motion of a rigid body fastened to a cable in deployment of the orbital cable system, etc.).

Zeros of Schrödinger's Radial Function Rnl(r) and Kummer's Function 1F1(-a c; z) and Their ``Angle'' Distributions

NASA Astrophysics Data System (ADS)

Tarasov, V. F.

In the present paper exact formulae for the calculation of zeros of Rnl(r) and 1F1(-a c; z), where z = 2 λ r, a = n - l - 1 >= 0 and c = 2l + 2 >= 2 are presented. For a <= 4 the method due to Tartallia and Cardono, and that due to L. Ferrai, L. Euler and J. L. Lagrange are used. In other cases (a > 4) numerical methods are employed to obtain the results (to within 10-15). For greater geometrical obviousness of the irregulary distribution (as a > 3) of zeros xk = zk - (c + a - 1) on the axis y = 0, the circular diagrams with the radius Ra = (a - 1) √ {c + a - 1} are presented for the first time. It is possible to notice some singularities of distribution of these zeros and their images - the points Tk - on the circle. For a = 3 and 4 their exact ``angle'' asymptotics (as c --> ∞) are obtained. It is shown that in the basis of the L. Ferrari, L. Euler and J.-L. Lagrange methods, using for solving the equation 1F1(-4 c; z) = 0, one Common for all these methods. equation is obtained viz., the cubic resolvent equation of FEL-type. Calculating of zeros xk of the Rnl(r) and 1F1(z) functions enable us to show the ``singular'' cases (a, c) = (4, 6), (6, 4), (8, 14), ...

The structure of carbon nanotubes formed of graphene layers L4-8, L5-7, L3-12, L4-6-12

NASA Astrophysics Data System (ADS)

Shapovalova, K. E.; Belenkov, E. A.

2017-11-01

We geometrically calculate the optimized structure of nanotubes based on the graphene layers, using the method of molecular mechanics MM+. It was found that only the nanotubes, based on the graphene layers L4-8, L5-7, L3-12, L4-6-12, have a cylindrical form. Calculations of the sublimation energy, carried out using the semi-empirical quantum-mechanic method PM3, show that energy increases with the increase of nanotube diameters.

Two-point correlation function for Dirichlet L-functions

NASA Astrophysics Data System (ADS)

Bogomolny, E.; Keating, J. P.

2013-03-01

The two-point correlation function for the zeros of Dirichlet L-functions at a height E on the critical line is calculated heuristically using a generalization of the Hardy-Littlewood conjecture for pairs of primes in arithmetic progression. The result matches the conjectured random-matrix form in the limit as E → ∞ and, importantly, includes finite-E corrections. These finite-E corrections differ from those in the case of the Riemann zeta-function, obtained in Bogomolny and Keating (1996 Phys. Rev. Lett. 77 1472), by certain finite products of primes which divide the modulus of the primitive character used to construct the L-function in question.

Identifying fMRI Model Violations with Lagrange Multiplier Tests

PubMed Central

Cassidy, Ben; Long, Christopher J; Rae, Caroline; Solo, Victor

2013-01-01

The standard modeling framework in Functional Magnetic Resonance Imaging (fMRI) is predicated on assumptions of linearity, time invariance and stationarity. These assumptions are rarely checked because doing so requires specialised software, although failure to do so can lead to bias and mistaken inference. Identifying model violations is an essential but largely neglected step in standard fMRI data analysis. Using Lagrange Multiplier testing methods we have developed simple and efficient procedures for detecting model violations such as non-linearity, non-stationarity and validity of the common Double Gamma specification for hemodynamic response. These procedures are computationally cheap and can easily be added to a conventional analysis. The test statistic is calculated at each voxel and displayed as a spatial anomaly map which shows regions where a model is violated. The methodology is illustrated with a large number of real data examples. PMID:22542665

On a Lagrange-Hamilton formalism describing position and momentum uncertainties

NASA Technical Reports Server (NTRS)

Schuch, Dieter

1993-01-01

According to Heisenberg's uncertainty relation, in quantum mechanics it is not possible to determine, simultaneously, exact values for the position and the momentum of a material system. Calculating the mean value of the Hamiltonian operator with the aid of exact analytic Gaussian wave packet solutions, these uncertainties cause an energy contribution additional to the classical energy of the system. For the harmonic oscillator, e.g., this nonclassical energy represents the ground state energy. It will be shown that this additional energy contribution can be considered as a Hamiltonian function, if it is written in appropriate variables. With the help of the usual Lagrange-Hamilton formalism known from classical particle mechanics, but now considering this new Hamiltonian function, it is possible to obtain the equations of motion for position and momentum uncertainties.

2,3-trans-3,4-trans-3,4-Dihydroxy-L-proline: An amino acid in toxic peptides of Amanita virosa mushrooms

PubMed Central

Buku, A.; Faulstich, H.; Wieland, T.; Dabrowski, J.

1980-01-01

Among the four possible stereoisomers of 3,4-dihydroxy-L-proline,2,3-trans-3,4-trans-3,4-dihydroxy-L-proline (IV) had not been found in nature previously. It has now been detected as a component of virotoxins, toxic peptides of Amanita virosa mushrooms. Because periodate failed to effect an oxidative glycol splitting reaction, the two hydroxyl groups in positions 3 and 4 were expected to be in a trans configuration. Furthermore, the formation of a 4-lactone on treatment with acids pointed to the carboxyl group and the hydroxyl group at position 4 being in a cis configuration. These results are in agreement with structure IV only. Final proof for structure IV was given by NMR spectroscopy and direct comparison with the 2,3-cis-3,4-trans-3,4-dihydroxy-L-proline isomer. PMID:16592813

Gene Expression Data to Mouse Atlas Registration Using a Nonlinear Elasticity Smoother and Landmark Points Constraints

PubMed Central

Lin, Tungyou; Guyader, Carole Le; Dinov, Ivo; Thompson, Paul; Toga, Arthur; Vese, Luminita

2013-01-01

This paper proposes a numerical algorithm for image registration using energy minimization and nonlinear elasticity regularization. Application to the registration of gene expression data to a neuroanatomical mouse atlas in two dimensions is shown. We apply a nonlinear elasticity regularization to allow larger and smoother deformations, and further enforce optimality constraints on the landmark points distance for better feature matching. To overcome the difficulty of minimizing the nonlinear elasticity functional due to the nonlinearity in the derivatives of the displacement vector field, we introduce a matrix variable to approximate the Jacobian matrix and solve for the simplified Euler-Lagrange equations. By comparison with image registration using linear regularization, experimental results show that the proposed nonlinear elasticity model also needs fewer numerical corrections such as regridding steps for binary image registration, it renders better ground truth, and produces larger mutual information; most importantly, the landmark points distance and L2 dissimilarity measure between the gene expression data and corresponding mouse atlas are smaller compared with the registration model with biharmonic regularization. PMID:24273381

THE POSSIBLE IMPACT OF L5 MAGNETOGRAMS ON NON-POTENTIAL SOLAR CORONAL MAGNETIC FIELD SIMULATIONS

DOE Office of Scientific and Technical Information (OSTI.GOV)

Weinzierl, Marion; Yeates, Anthony R.; Mackay, Duncan H.

The proposed Carrington-L5 mission would bring instruments to the L5 Lagrange point to provide us with crucial data for space weather prediction. To assess the importance of including a magnetograph, we consider the possible differences in non-potential solar coronal magnetic field simulations when magnetograph observations are available from the L5 point, compared with an L1-based field of view (FOV). A timeseries of synoptic radial magnetic field maps is constructed to capture the emergence of two active regions from the L5 FOV. These regions are initially absent in the L1 magnetic field maps, but are included once they rotate into themore » L1 FOV. Non-potential simulations for these two sets of input data are compared in detail. Within the bipolar active regions themselves, differences in the magnetic field structure can exist between the two simulations once the active regions are included in both. These differences tend to reduce within 5 days of the active region being included in L1. The delayed emergence in L1 can, however, lead to significant persistent differences in long-range connectivity between the active regions and the surrounding fields, and also in the global magnetic energy. In particular, the open magnetic flux and the location of open magnetic footpoints, are sensitive to capturing the real-time of emergence. These results suggest that a magnetograph at L5 could significantly improve predictions of the non-potential corona, the interplanetary magnetic field, and of solar wind source regions on the Sun.« less

Analysis of complex elastic structures by a Rayleigh-Ritz component modes method using Lagrange multipliers. Ph.D. Thesis

NASA Technical Reports Server (NTRS)

Klein, L. R.

1974-01-01

The free vibrations of elastic structures of arbitrary complexity were analyzed in terms of their component modes. The method was based upon the use of the normal unconstrained modes of the components in a Rayleigh-Ritz analysis. The continuity conditions were enforced by means of Lagrange Multipliers. Examples of the structures considered are: (1) beams with nonuniform properties; (2) airplane structures with high or low aspect ratio lifting surface components; (3) the oblique wing airplane; and (4) plate structures. The method was also applied to the analysis of modal damping of linear elastic structures. Convergence of the method versus the number of modes per component and/or the number of components is discussed and compared to more conventional approaches, ad-hoc methods, and experimental results.

[L-THIA-based management design for controlling urban non-point source pollution].

PubMed

Guo, Qing-Hai; Yang, Liu; Ke-Ming, Ma

2007-11-01

L-THIA Model was used to simulate the amounts of NPS pollutants in 2 catchments of Sanjiao watershed (Sj1, Sj2) in Hanyang district, and the total simulated amount of NPS loads in Sj1 and Sj2 were 1.82 x 10(4) kg, 1.38 x 10(5) kg, respectively. Based on the theory of resource-sink" and interaction of pattern with process, a series of BMPs, including green roof, grassland, porous pavement, infiltration trench, vegetative filter strip and wet pond, were optimized, and effects of BMPs were simulated along the surface runoff pathway. The results show that total pollutants outputs entering Sj1 and Sj2 account for 14.65% and 6.57%, respectively. Combining L-THIA model and BMPs in series is a proper measure for non-point source pollution control and urban development planning at watershed or region scale.

Smoot Cosmology Group

Science.gov Websites

orbit around L2, the second Lagrange point of the Earth-Sun system, which is about 1.5 million orbits L2, it makes one rotation about the Sun per year. The spacecraft spin axis has to be rotated at the same rate in order to remain Sun pointed. This is achieved by making regular manoeuvres that will

The morphometric study of l3-L4 and L4-L5 lumbar spine in Asian population using magnetic resonance imaging: feasibility analysis for transpsoas lumbar interbody fusion.

PubMed

Yusof, Mohd Imran; Nadarajan, Eswaran; Abdullah, Mohd Shafie

2014-06-15

Cross-sectional study on the measurement of relevant magnetic resonance imaging parameters in 100 patients presented for lumbar spine assessment. To determine anatomical position of lumbar plexus and major blood vessels in relation to vertebral body and anterior edge of psoas muscle at L3-L4 and L4-L5 and to define the safe working zone for transpsoas approach for lumbar fusion. Lateral transpsoas lumbar interbody fusion has been shown to be safe and provides alternative for lumbar fusion. However, proximity of neurovascular structures may not allow a safe passage for this procedure in the Asian population. Relevant parameters were measured from axial magnetic resonance images and analyzed, including the psoas muscle and vertebrae endplate diameters, lumbar plexus and psoas muscle distance, lumbar plexus and vertebra body distance, and vena cava to the anterior vertebrae body diameters. The mean anteroposterior diameters of the right and left psoas muscle ranged from 44.0 to 58.6 mm and 44.8 to 54.0 mm, respectively. The mean anteroposterior diameters of vertebra endplate of L3, L4, and L5 were 38.2 mm, 39.3 mm, and 41.4 mm, respectively. The mean distance of posterior border of vena cava from the vertebra body was 4.5 mm at L3-L4 and 14.1 mm at L4-L5. L3-L4 fusion is feasible at both sides in both sexes; however, at L4-L5 level, the procedure is feasible only on the left side. The safe working zone for transpsoas approach to lumbar spine is significantly narrower at L4-L5 in both sexes. Anterior edge of psoas muscle can be used as a reliable guide to locate lumbar plexus within psoas muscle. N/A.

Spacecraft Maneuvering at the Sun/Earth-Moon L2 Libration Point

NASA Astrophysics Data System (ADS)

Shahid, Kamran

Spacecraft formation flying in the vicinity of the Sun/Earth-Moon libration points offers many promising possibilities for space exploration. The concept of formation flying involves the distribution of the functionality of a single spacecraft among several smaller, cooperative spacecraft. The libration points are locations relative to two large orbiting bodies where a third body with relatively small mass can remain stationary relative to the two larger bodies. The most significant perturbation experienced by a spacecraft at the libration point is effect of solar radiation pressure. This thesis presents the development of nonlinear control techniques for maneuvering control at the Sun-Earth/Moon L2 libration point. A new thruster based formation control technique is presented. We also consider a leader/follower formation architecture, and examine the station keeping control of the leader spacecraft and the formation control of the follower spacecraft using solar radiation pressure. Reference trajectories of the leader spacecraft, halo and Lissajous orbits, are determined using a numerical technique in order to take into account all major gravitational perturbations. The nonlinear controllers are developed based on Lyapunov analysis, including non-adaptive and adaptive designs. Thruster based and solar radiation pressure based control laws for spacecraft maneuvering at the Sun-Earth/Moon libration point are developed. Higher order sliding mode control is utilized to address the non-affine structure of the solar sail control inputs. The reduced input solar radiation pressure problem is properly addressed as an underactuated control problem. The development of adaptive control for solar sail equipped spacecraft is an innovation and represents and advancement in solar sailing control technology. Controller performance is evaluated in a high fidelity ephemeris model to reflect a realistic simulated space environment. The numerical results demonstrate the effectiveness

Erratum: ``The Luminosity Function of IRAS Point Source Catalog Redshift Survey Galaxies'' (ApJ, 587, L89 [2003])

NASA Astrophysics Data System (ADS)

Takeuchi, Tsutomu T.; Yoshikawa, Kohji; Ishii, Takako T.

2004-05-01

We have mentioned that we normalized the parameters for the luminosity function by the Hubble constant H0=100 km s-1 Mpc-1 however, for the characteristic luminosity L* we erroneously normalized it by H0=70 km s-1 Mpc-1. As a result, we have proposed wrong numerical factors for L*. In addition, there is a typographic error in the exponent of equation (6) of the published manuscript. Correct values are as follows: L*=(4.34+/-0.86)×108 h-2 [Lsolar] for equation (4), and L*=(2.50+/-0.44)×109 h-2 [Lsolar] and L*=(9.55+/-0.20)×108 h-2 [Lsolar] for equations (5) and (6), respectively. All the other parameters are correct. The errors have occurred only in the final conversion, and they do not affect our discussions and conclusions at all. We thank P. Ranalli for pointing out the errors.

Application of parallel distributed Lagrange multiplier technique to simulate coupled Fluid-Granular flows in pipes with varying Cross-Sectional area

DOE PAGES

Kanarska, Yuliya; Walton, Otis

2015-11-30

Fluid-granular flows are common phenomena in nature and industry. Here, an efficient computational technique based on the distributed Lagrange multiplier method is utilized to simulate complex fluid-granular flows. Each particle is explicitly resolved on an Eulerian grid as a separate domain, using solid volume fractions. The fluid equations are solved through the entire computational domain, however, Lagrange multiplier constrains are applied inside the particle domain such that the fluid within any volume associated with a solid particle moves as an incompressible rigid body. The particle–particle interactions are implemented using explicit force-displacement interactions for frictional inelastic particles similar to the DEMmore » method with some modifications using the volume of an overlapping region as an input to the contact forces. Here, a parallel implementation of the method is based on the SAMRAI (Structured Adaptive Mesh Refinement Application Infrastructure) library.« less

Synthesis of cis-4-trifluoromethyl- and cis-4-difluoromethyl-l-pyroglutamic acids.

PubMed

Qiu, Xiao-Long; Qing, Feng-Ling

2003-05-02

Efforts to synthesize 4-trifluoromethyl- and 4-difluoromethyl-l-pyroglutamic acids are described. After many arduous efforts, we successfully synthesized our target molecules cis-4-trifluoromethyl-l-pyroglutamic acid 25 and cis-4-difluoromethyl-l-pyroglutamic acid 26 from trans-4-hydroxy-l-proline through oxidation of fluorinated prolinates with RuO(4).

Robust adaptive uniform exact tracking control for uncertain Euler-Lagrange system

NASA Astrophysics Data System (ADS)

Yang, Yana; Hua, Changchun; Li, Junpeng; Guan, Xinping

2017-12-01

This paper offers a solution to the robust adaptive uniform exact tracking control for uncertain nonlinear Euler-Lagrange (EL) system. An adaptive finite-time tracking control algorithm is designed by proposing a novel nonsingular integral terminal sliding-mode surface. Moreover, a new adaptive parameter tuning law is also developed by making good use of the system tracking errors and the adaptive parameter estimation errors. Thus, both the trajectory tracking and the parameter estimation can be achieved in a guaranteed time adjusted arbitrarily based on practical demands, simultaneously. Additionally, the control result for the EL system proposed in this paper can be extended to high-order nonlinear systems easily. Finally, a test-bed 2-DOF robot arm is set-up to demonstrate the performance of the new control algorithm.

Relative Critical Points

NASA Astrophysics Data System (ADS)

Lewis, Debra

2013-05-01

Relative equilibria of Lagrangian and Hamiltonian systems with symmetry are critical points of appropriate scalar functions parametrized by the Lie algebra (or its dual) of the symmetry group. Setting aside the structures - symplectic, Poisson, or variational - generating dynamical systems from such functions highlights the common features of their construction and analysis, and supports the construction of analogous functions in non-Hamiltonian settings. If the symmetry group is nonabelian, the functions are invariant only with respect to the isotropy subgroup of the given parameter value. Replacing the parametrized family of functions with a single function on the product manifold and extending the action using the (co)adjoint action on the algebra or its dual yields a fully invariant function. An invariant map can be used to reverse the usual perspective: rather than selecting a parametrized family of functions and finding their critical points, conditions under which functions will be critical on specific orbits, typically distinguished by isotropy class, can be derived. This strategy is illustrated using several well-known mechanical systems - the Lagrange top, the double spherical pendulum, the free rigid body, and the Riemann ellipsoids - and generalizations of these systems.

Augmented Lagrange Programming Neural Network for Localization Using Time-Difference-of-Arrival Measurements.

PubMed

Han, Zifa; Leung, Chi Sing; So, Hing Cheung; Constantinides, Anthony George

2017-08-15

A commonly used measurement model for locating a mobile source is time-difference-of-arrival (TDOA). As each TDOA measurement defines a hyperbola, it is not straightforward to compute the mobile source position due to the nonlinear relationship in the measurements. This brief exploits the Lagrange programming neural network (LPNN), which provides a general framework to solve nonlinear constrained optimization problems, for the TDOA-based localization. The local stability of the proposed LPNN solution is also analyzed. Simulation results are included to evaluate the localization accuracy of the LPNN scheme by comparing with the state-of-the-art methods and the optimality benchmark of Cramér-Rao lower bound.

17. View to west. Detail, connection point L2 (see plans), ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

17. View to west. Detail, connection point L2 (see plans), from below deck. (135mm lens) - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

Analysis of the heat capacity for pure CH4 and CH4/CCl4 on graphite near the melting point and calculation of the T-X phase diagram for (CH3)CCl3 + CCl4

NASA Astrophysics Data System (ADS)

Yurtseven, Hamit; Yılmaz, Aygül

2016-06-01

We study the temperature dependence of the heat capacity Cp for the pure CH4 and the coadsorbed CH4/CCl4 on graphite near the melting point. The heat capacity peaks are analyzed using the experimental data from the literature by means of the power-law formula. The critical exponents for the heat capacity are deduced below and above the melting point for CH4 (Tm = 104.8 K) and CH4/CCl4 (Tm = 99.2 K). Our exponent values are larger as compared with the predicted values of some theoretical models exhibiting second order transition. Our analyses indicate that the pure methane shows a nearly second order (weak discontinuity in the heat capacity peak), whereas the transition in coadsorbed CH4/CCl4 is of first order (apparent discontinuity in Cp). We also study the T - X phase diagram of a two-component system of CH3CCl3+CCl4 using the Landau phenomenological model. Phase lines of the R+L (rhombohedral+liquid) and FCC+L (face-centred cubic + liquid) are calculated using the observed T - X phase diagram of this binary mixture. Our results show that the Landau mean field theory describes the observed behavior of CH3CCl3+CCl4 adequately. From the calculated T - X phase diagram, critical behavior of some thermodynamic quantities can be predicted at various temperatures and concentrations (CCl4) for a binary mixture of CH3CCl3+CCl4.

Biomechanics of 4-point seat belt systems in frontal impacts.

PubMed

Rouhana, Stephen W; Bedewi, Paul G; Kankanala, Sundeep V; Prasad, Priya; Zwolinski, Joseph J; Meduvsky, Alex G; Rupp, Jonathan D; Jeffreys, Thomas A; Schneider, Lawrence W

2003-01-01

The biomechanical behavior of 4-point seat belt systems was investigated through MADYMO modeling, dummy tests and post mortem human subject tests. This study was conducted to assess the effect of 4-point seat belts on the risk of thoracic injury in frontal impacts, to evaluate the ability to prevent submarining under the lap belt using 4-point seat belts, and to examine whether 4-point belts may induce injuries not typically observed with 3-point seat belts. The performance of two types of 4-point seat belts was compared with that of a pretensioned, load-limited, 3-point seat belt. A 3-point belt with an extra shoulder belt that "crisscrossed" the chest (X4) appeared to add constraint to the torso and increased chest deflection and injury risk. Harness style shoulder belts (V4) loaded the body in a different biomechanical manner than 3-point and X4 belts. The V4 belt appeared to shift load to the clavicles and pelvis and to reduce traction of the shoulder belt across the chest, resulting in a reduction in chest deflection by a factor of two. This is associated with a 5 to 500-fold reduction in thoracic injury risk, depending on whether one assumes 4-point belts apply concentrated or distributed load. In four of six post mortem human subjects restrained by V4 belts during 40 km/h sled tests, chest compression was zero or negative and rib fractures were nearly eliminated. Submarining was not observed in any test with post mortem human subjects. Though lumbar, sacral and pelvic injuries were noted, they are believed to be due to the artificial restraint environment (no knee bolsters, instrument panels, steering systems or airbags). While they show significant potential to reduce thoracic injury risk, there are still many issues to be resolved before 4-point belts can be considered for production vehicles. These issues include, among others, potential effects on hard and soft neck tissues, of interaction with inboard shoulder belts in farside impacts and potential

Regulation of human adenovirus alternative RNA splicing by the adenoviral L4-33K and L4-22K proteins.

PubMed

Biasiotto, Roberta; Akusjärvi, Göran

2015-01-28

Adenovirus makes extensive use of alternative RNA splicing to produce a complex set of spliced viral mRNAs. Studies aimed at characterizing the interactions between the virus and the host cell RNA splicing machinery have identified three viral proteins of special significance for the control of late viral gene expression: L4-33K, L4-22K, and E4-ORF4. L4-33K is a viral alternative RNA splicing factor that controls L1 alternative splicing via an interaction with the cellular protein kinases Protein Kinase A (PKA) and DNA-dependent protein kinase (DNA-PK). L4-22K is a viral transcription factor that also has been implicated in the splicing of a subset of late viral mRNAs. E4-ORF4 is a viral protein that binds the cellular protein phosphatase IIA (PP2A) and controls Serine/Arginine (SR)-rich protein activity by inducing SR protein dephosphorylation. The L4-33K, and most likely also the L4-22K protein, are highly phosphorylated in vivo. Here we will review the function of these viral proteins in the post-transcriptional control of adenoviral gene expression and further discuss the significance of potential protein kinases phosphorylating the L4-33K and/or L4-22K proteins.

A Radiographic Measurement of the Anterior Epidural Space at L4-5 Disc Level.

PubMed

Xu, Rui-Sheng; Wu, Jie-Shi; Lu, Hai-Dan; Zhu, Hao-Gang; Li, Xia; Dong, Jian; Yuan, Feng-Lai

2017-05-01

To observe the morphology character of the anterior epidural space at the L 4-5 disc level and to provide an anatomical basis for safely and accurately performing a percutaneous endoscopic lumbar discectomy (PELD). Fifty-five cases with L 5 S 1 lumbar disc herniation were included in this study, and cases with L 4-5 disease were excluded. When the puncture needle reached the epidural space at the L 5 S 1 level, iohexol was injected at the pressure of 50 cm H 2 O during the PELD, then C-Arm fluoroscopy was used to obtain standard lumbar frontal and lateral images. The widths of epidural space at the level of the L 4 lower endplate, the L 5 upper endplate, as well as the middle point of the L 4-5 disc were measured from the lumbar lateral X-ray film. Epidural space at the L 4-5 disc plane performs like a trapezium chart with a short side at the head end and a long side at the tail end in the lumbar lateral X-ray radiograph, while the average widths of epidural space were 10.2 ± 2.5, 12.3 ± 2.3, and 13.8 ± 2.6 mm at the upper, middle, and lower level of the L 4-5 disc. Understanding the morphological characteristics of epidural space will contribute to improving the safety of the tranforaminal percutaneous endoscopy technique. © 2017 Chinese Orthopaedic Association and John Wiley & Sons Australia, Ltd.

Dynamical evolution of a fictitious population of binary Neptune Trojans

NASA Astrophysics Data System (ADS)

Brunini, Adrián

2018-03-01

We present numerical simulations of the evolution of a synthetic population of Binary Neptune Trojans, under the influence of the solar perturbations and tidal friction (the so-called Kozai cycles and tidal friction evolution). Our model includes the dynamical influence of the four giant planets on the heliocentric orbit of the binary centre of mass. In this paper, we explore the evolution of initially tight binaries around the Neptune L4 Lagrange point. We found that the variation of the heliocentric orbital elements due to the libration around the Lagrange point introduces significant changes in the orbital evolution of the binaries. Collisional processes would not play a significant role in the dynamical evolution of Neptune Trojans. After 4.5 × 109 yr of evolution, ˜50 per cent of the synthetic systems end up separated as single objects, most of them with slow diurnal rotation rate. The final orbital distribution of the surviving binary systems is statistically similar to the one found for Kuiper Belt Binaries when collisional evolution is not included in the model. Systems composed by a primary and a small satellite are more fragile than the ones composed by components of similar sizes.

A novel formulation of L-thyroxine (L-T4) reduces the problem of L-T4 malabsorption by coffee observed with traditional tablet formulations.

PubMed

Vita, Roberto; Saraceno, Giovanna; Trimarchi, Francesco; Benvenga, Salvatore

2013-02-01

The purpose of this work is to evaluate if the coffee-associated malabsorption of tablet levothyroxine (L-T4) is reduced by soft gel capsule. We recruited 8 patients with coffee-associated L-T4 malabsorption including one hypothyroid patient. For 6 months, the patients were switched to the capsule maintaining the L-T4 daily dose. Patients took the capsule with water, having coffee 1 h later (proper habit, PH) on days 1-90, or with coffee ≤ 5 min later (improper habit, IH) on days 91-180. After 6 months, 2 patients volunteered for an acute loading test of 600 μg L-T4 (capsule) ingested with water (PH) or with coffee (IH). In the single hypothyroid patient, the post-switch TSH ranged 0.06-0.16 mU/L (PH) versus 5.8-22.4 mU/L pre-switch (PH) and 0.025-0.29 mU/L (IH) versus 26-34 mU/L pre-switch (IH). In the other 7 patients, post-switch TSH was 0.41 ± 0.46 (PH) versus 0.28 ± 0.20 pre-switch (PH) (P = 0.61) and 0.34 ± 0.30 (IH) versus 1.23 ± 1.47 pre-switch (IH) (P < 0.001). Importantly, TSH levels in PH versus IH habit did not differ post-switch (P = 0.90), but they did pre-switch (P < 0.0001). The proportions of post-switch TSH levels <0.10 mU/L with PH (33.3 %) or with IH (33.3 %) were borderline significantly greater than the corresponding pre-switch levels with PH (10.3 %) (P = 0.088) or with IH (0 %) (P = 0.0096). In the two volunteers, the L-T4 loading test showed that coffee influenced L-T4 pharmacokinetics minimally. Soft gel capsules can be used in patients who are unable/unwilling to change their IH of taking L-T4.

Variational tricomplex of a local gauge system, Lagrange structure and weak Poisson bracket

NASA Astrophysics Data System (ADS)

Sharapov, A. A.

2015-09-01

We introduce the concept of a variational tricomplex, which is applicable both to variational and nonvariational gauge systems. Assigning this tricomplex with an appropriate symplectic structure and a Cauchy foliation, we establish a general correspondence between the Lagrangian and Hamiltonian pictures of one and the same (not necessarily variational) dynamics. In practical terms, this correspondence allows one to construct the generating functional of a weak Poisson structure starting from that of a Lagrange structure. As a byproduct, a covariant procedure is proposed for deriving the classical BRST charge of the BFV formalism by a given BV master action. The general approach is illustrated by the examples of Maxwell’s electrodynamics and chiral bosons in two dimensions.

Buhne Point Shoreline Erosion Demonstration Project. Volume 4. Appendices H-L.

DTIC Science & Technology

1987-08-01

Districts Corps of Engineers Prepared by Moffatt & Nichol, EngineE -s 250 W. Wardlow Road Long Beach, CA 90807 L-2434.03 August 1987...Francisco District, Corps of Engineers Dave Eyres Federal Highway Administration Ervin Renner Humboldt County Board of Supervisors Tom Smith Federal...James A. Gast Humboldt Bay Harbor District Claude Wong U.S. Army Corps of Engineers , Los Angeles Dean Ray Coastal Commission, Eureka Ervin Renner

Getting to L1 the Hard Way: Triana's Launch Options

NASA Technical Reports Server (NTRS)

Houghton, Martin B.; Bauer, Frank H. (Technical Monitor)

2002-01-01

Over the past four years, NASA's Goddard Space Flight Center has built and tested the Triana observatory, which will be the first Earth observing science satellite to take advantage of the unique perspective offered by a Lissajous orbit about the first Earth-Sun Lagrange Point (L1). Triana was originally meant to fly on the U.S. Space Transportation System (a.k.a. the Space Shuttle but complications with the shuttle manifest have forced Triana into a 'wait and see' attitude. The observatory is currently being stored at NASA's Goddard Space Flight Center, where it waits for an appropriate launch opportunity to surface. To that end, several possible alternatives have been considered, including variations on the nominal shuttle deployment scenario, a high inclination Delta-type launch from Vandenberg Air Force Base, a Tsyklon class vehicle launched from Baikonur, Kazakhstan, and a ride on a French Ariane vehicle out of French Guiana into a somewhat arbitrary geostationary transfer orbit (GTO). This paper chronicles and outlines the pros and cons of how each of these opportunities could be used to send Triana on its way to L1.

Getting to L1 the Hard Way: Triana's Launch Options

NASA Technical Reports Server (NTRS)

Houghton, Martin B.; Bauer, Frank H. (Technical Monitor)

2002-01-01

Over the past four years, NASA's Goddard Space Flight Center has built and tested the Triana observatory, which will be the first Earth observing science satellite to take advantage of the unique perspective offered by a Lissajous orbit about the first Earth-Sun Lagrange Point (L1). Triana was originally meant to fly on the U.S. Space Transportation System (a.k.a. the Space Shuttle), but complications with the shuttle manifest have forced Triana into a "wait and see" attitude. The observatory is currently being stored at NASA's Goddard Space Flight Center, where it waits for an appropriate launch opportunity to materialize. To that end, several possible alternatives have been considered, including variations on the nominal shuttle deployment scenario, a high inclination Delta-type launch from Vandenberg Air Force Base, a Tsyklon class vehicle launched from Baikonur, Kazakhstan, and a ride on a French Ariane vehicle out of French Guiana into a somewhat arbitrary geostationary transfer orbit (GTO). This paper chronicles and outlines the pros and cons of how each of these opportunities could be used to send Triana on its way to L1.

Trehalose catabolism enzymes in L3 and L4 larvae of Anisakis simplex.

PubMed

Lopieńska-Biernat, E; Zółtowska, K; Rokicki, J

2007-12-01

The presence of trehalase and trehalose phosphorylase in L3 and L4 larvae of Anisakis simplex was demonstrated. The activity of trehalase and trehalose phosphorylase in L3 larvae was 6 and 10 times higher, respectively, than in L4 larvae. This suggests that trehalose metabolism is more important for L3 than LA larvae. Trehalases of L3 and L4 differ in their characteristics. The enzyme of L3 was present mainly in the lysosomes and cytosol, whereas in L4 the highest enzyme activity was measured in the lysosomal fraction. Trehalase activity was increased by 29% in L3 and 55% in L4 with the addition of Mg2+ (0.1 mmol). Tris inhibited trehalase in L3 larvae by 42% and in L4 by 25%. The enzymes differed in their reaction to EDTA, CaCl2, ZnCl2, and CH2ICOOH (all 0.1 mmol). High activity of trehalase from L3 larvae was measured within the pH range of 5.0 to 6.5, with an optimum pH of 6.1. The trehalase was a thermally tolerant enzyme from 25 C to 60 C. The enzyme lost half of its activity after preincubation without substrate above 75 C. The paper also discusses the similarities and differences in characteristics of trehalase from A. simplex larvae and presents the comparison to enzymes from other nematodes.

Significance of specific force models in two applications: Solar sails to sun-earth L4/L5 and grail data analysis suggesting lava tubes and buried craters on the moon

NASA Astrophysics Data System (ADS)

Sood, Rohan

In the trajectory design process, gravitational interaction between the bodies of interest plays a key role in developing the over-arching force model. However, non-gravitational forces, such as solar radiation pressure (SRP), can significantly influence the motion of a spacecraft. Incorporating SRP within the dynamical model can assist in estimating the trajectory of a spacecraft with greater precision, in particular, for a spacecraft with a large area-to-mass ratio, i.e., solar sails. Subsequently, in the trajectory design process, solar radiation pressure can be leveraged to maneuver the sail-based spacecraft. First, to construct low energy transfers, the invariant manifolds are explored that form an important tool in the computation and design of complex trajectories. The focus is the investigation of trajectory design options, incorporating solar sail dynamics, from the Earth parking orbit to the vicinity of triangular Lagrange points. Thereafter, an optimization scheme assisted in investigating the ?V requirement to depart from the Earth parking orbit. Harnessing the solar radiation pressure, the spacecraft is delivered to the vicinity of the displaced Lagrange point and maintains a trajectory close to the artificial libration point with the help of the solar sail. However, these trajectories are converged in a model formulated as a three-body problem with additional acceleration from solar radiation pressure. Thus, the trajectories are transitioned to higher fidelity ephemeris model to account for additional perturbing accelerations that may dominate the sail-craft dynamics and improve upon the trajectory design process. Alternatively, precise knowledge of the motion of a spacecraft about a central body and the contribution of the SRP can assist in deriving a highly accurate gravity field model. The high resolution gravity data can potentially assist in exploring the surface and subsurface properties of a particular body. With the goal of expanding human

Reduction of [Cp*Sb]4 with Subvalent Main-Group Metal Reductants: Syntheses and Structures of [(L1 Mg)4 (Sb4 )] and [(L2 Ga)2 (Sb4 )] Containing Edge-Missing Sb4 Units.

PubMed

Ganesamoorthy, Chelladurai; Krüger, Julia; Wölper, Christoph; Nizovtsev, Anton S; Schulz, Stephan

2017-02-16

[Cp*Sb] 4 (Cp*=C 5 Me 5 ) reacts with [L 1 Mg] 2 and L 2 Ga with formation of [(L 1 Mg) 4 (μ 4 ,η 1:2:2:2 -Sb 4 )] (L 1 =iPr 2 NC[N(2,6-iPr 2 C 6 H 3 )] 2 , 1) and [(L 2 Ga) 2 (μ,η 2:2 -Sb 4 )] (L 2 =HC[C(Me)N(2,6-iPr 2 C 6 H 3 )] 2 , 2). The cleavage of the Sb-Sb and Sb-C bonds in [Cp*Sb] 4 are the crucial steps in both reactions. The formation of 1 occurred by elimination of the Cp* anion and formation of Cp*MgL 1 , while 2 was formed by reductive elimination of Cp* 2 and oxidative addition of L 2 Ga to the Sb 4 unit. 1 and 2 were characterized by heteronuclear NMR spectroscopy and single-crystal X-ray diffraction, and their bonding situation was studied by quantum chemical calculations. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

James Webb Space Telescope - L2 Communications for Science Data Processing

NASA Technical Reports Server (NTRS)

Johns, Alan; Seaton, Bonita; Gal-Edd, Jonathan; Jones, Ronald; Fatig, Curtis; Wasiak, Francis

2008-01-01

JWST is the first NASA mission at the second Lagrange point (L2) to identify the need for data rates higher than 10 megabits per second (Mbps). JWST will produce approximately 235 Gigabits of science data every day that will be downlinked to the Deep Space Network (DSN). To get the data rates desired required moving away from X-band frequencies to Ka-band frequencies. To accomplish this transition, the DSN is upgrading its infrastructure. This new range of frequencies are becoming the new standard for high data rate science missions at L2. With the new frequency range, the issues of alternatives antenna deployment, off nominal scenarios, NASA implementation of the Ka-band 26 GHz, and navigation requirements will be discussed in this paper. JWST is also using Consultative Committee for Space Data Systems (CCSDS) standard process for reliable file transfer using CCSDS File Delivery Protocol (CFDP). For JWST the use of the CFDP protocol provides level zero processing at the DSN site. This paper will address NASA implementations of Ground Stations in support of Ka-band 26 GHz and lesson learned from implementing a file base (CFDP) protocol operational system.

The Scientific Value and Technical Challenge of Chang'E-4 Landing on the Far-side of the Moon

NASA Astrophysics Data System (ADS)

Li, Fei; Zhang, He; Wu, Xueying; Ma, Jinan; Zhou, Wenyan

2016-07-01

The mission of Chang'E-4 probe is landing on the far-side of the moon for the first time in human history. Compared with the near-side, far-side landing has unique scientific value and more challenging technology implementation. The scientific significance of the exploration of far-side of the moon and the technical difficulties and solution approach of the Chang'E-4 were discussed in this paper. In science, the far-side of the Moon is an ideal scientific platform in that it is shielded from terrestrial radio-frequency interference. The terrane that records the formation of the primordial crust is located largely on the far-side hemisphere, and there is the largest and oldest recognized impact basin in our Solar System, the South Pole-Aitken basin. In technology, the difficulties mainly includes the analysis of the influence of lunar topography of far-side on the landing descent strategy, the study of relay orbit and operating modes for the relay satellite in the Earth-Moon L2(Lagrange point 2), and the requirement analysis of autonomous management due to the relay communication. It will provide the reference for landing exploration of the far-side of the moon.

Combination L-T3 and L-T4 therapy for hypothyroidism.

PubMed

Wartofsky, Leonard

2013-10-01

Because of the longstanding controversy regarding whether hypothyroid patients can be optimally replaced by treatment with levothyroxine (L-T4) alone, numerous studies have addressed potential benefits of combined therapy of triiodothyronine (T3) with L-T4. Results of these studies have failed to support a potential benefit of combined therapy. A strong argument for the addition of L-T3 to L-T4 monotherapy has been lacking until recent genetic studies indicated a rationale for such therapy among a small fraction of the hypothyroid patient population. Interest in this issue has focused on the importance of the deiodinases in maintaining the euthyroid state and the role of genetic polymorphisms in the deiodinase genes that would affect thyroid hormone concentrations in both blood and tissues. One such polymorphism in the D2 gene, Thr92Ala, is associated with reduced T4 to T3 activation in skeletal muscle and thyroid, linked to obesity and alterations in thyroid-pituitary feedback, and in responses to thyroid hormone treatment. Although our professional organizations continue to recommend L-T4 alone for the treatment of hypothyroidism, the possibility of a D2 gene polymorphism should be considered in patients on L-T4 monotherapy who continue to complain of fatigue in spite of dosage achieving low normal serum thyroid stimulating hormone levels. A suggestive clue to the presence of this polymorphism could be a higher than normal free T4/free T3 ratio. Clinicians could consider adding T3 as a therapeutic trial in selected patients. Future well controlled clinical trials will be required to more fully resolve the controversy.

Orbit Determination Error Analysis Results for the Triana Sun-Earth L2 Libration Point Mission

NASA Technical Reports Server (NTRS)

Marr, G.

2003-01-01

Using the NASA Goddard Space Flight Center's Orbit Determination Error Analysis System (ODEAS), orbit determination error analysis results are presented for all phases of the Triana Sun-Earth L1 libration point mission and for the science data collection phase of a future Sun-Earth L2 libration point mission. The Triana spacecraft was nominally to be released by the Space Shuttle in a low Earth orbit, and this analysis focuses on that scenario. From the release orbit a transfer trajectory insertion (TTI) maneuver performed using a solid stage would increase the velocity be approximately 3.1 km/sec sending Triana on a direct trajectory to its mission orbit. The Triana mission orbit is a Sun-Earth L1 Lissajous orbit with a Sun-Earth-vehicle (SEV) angle between 4.0 and 15.0 degrees, which would be achieved after a Lissajous orbit insertion (LOI) maneuver at approximately launch plus 6 months. Because Triana was to be launched by the Space Shuttle, TTI could potentially occur over a 16 orbit range from low Earth orbit. This analysis was performed assuming TTI was performed from a low Earth orbit with an inclination of 28.5 degrees and assuming support from a combination of three Deep Space Network (DSN) stations, Goldstone, Canberra, and Madrid and four commercial Universal Space Network (USN) stations, Alaska, Hawaii, Perth, and Santiago. These ground stations would provide coherent two-way range and range rate tracking data usable for orbit determination. Larger range and range rate errors were assumed for the USN stations. Nominally, DSN support would end at TTI+144 hours assuming there were no USN problems. Post-TTI coverage for a range of TTI longitudes for a given nominal trajectory case were analyzed. The orbit determination error analysis after the first correction maneuver would be generally applicable to any libration point mission utilizing a direct trajectory.

Expression and function of AtMBD4L, the single gene encoding the nuclear DNA glycosylase MBD4L in Arabidopsis.

PubMed

Nota, Florencia; Cambiagno, Damián A; Ribone, Pamela; Alvarez, María E

2015-06-01

DNA glycosylases recognize and excise damaged or incorrect bases from DNA initiating the base excision repair (BER) pathway. Methyl-binding domain protein 4 (MBD4) is a member of the HhH-GPD DNA glycosylase superfamily, which has been well studied in mammals but not in plants. Our knowledge on the plant enzyme is limited to the activity of the Arabidopsis recombinant protein MBD4L in vitro. To start evaluating MBD4L in its biological context, we here characterized the structure, expression and effects of its gene, AtMBD4L. Phylogenetic analysis indicated that AtMBD4L belongs to one of the seven families of HhH-GPD DNA glycosylase genes existing in plants, and is unique on its family. Two AtMBD4L transcripts coding for active enzymes were detected in leaves and flowers. Transgenic plants expressing the AtMBD4L:GUS gene confined GUS activity to perivascular leaf tissues (usually adjacent to hydathodes), flowers (anthers at particular stages of development), and the apex of immature siliques. MBD4L-GFP fusion proteins showed nuclear localization in planta. Interestingly, overexpression of the full length MBD4L, but not a truncated enzyme lacking the DNA glycosylase domain, induced the BER gene LIG1 and enhanced tolerance to oxidative stress. These results suggest that endogenous MBD4L acts on particular tissues, is capable of activating BER, and may contribute to repair DNA damage caused by oxidative stress. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

Design and Comparison of Cascaded H-Bridge, Modular Multilevel Converter, and 5-L Active Neutral Point Clamped Topologies for Motor Drive Applications

DOE Office of Scientific and Technical Information (OSTI.GOV)

Marzoughi, Alinaghi; Burgos, Rolando; Boroyevich, Dushan

This paper presents the design procedure and comparison of converters currently used in medium-voltage high-power motor drive applications. For this purpose, the cascaded H-bridge (CHB), modular multilevel converter (MMC), and five-level active neutral point clamped (5-L ANPC) topologies are targeted. The design is performed using 1.7-kV insulated gate bipolar transistors (IGBTs) for CHB and MMC converters, and utilizing 3.3- and 4.5-kV IGBTs for 5-L ANPC topology as normally done in industry. The comparison is done between the designed converter topologies at three different voltage levels (4.16, 6.9, and 13.8 kV, with only the first two voltage levels in case ofmore » the 5-L ANPC) and two different power levels (3 and 5 MVA), in order to elucidate the dependence of different parameters on voltage and power rating. Finally, the comparison is done from several points of view such as efficiency, capacitive energy storage, semiconductor utilization, parts count (for measure of reliability), and power density.« less

Particle Swarm Optimization of Low-Thrust, Geocentric-to-Halo-Orbit Transfers

NASA Astrophysics Data System (ADS)

Abraham, Andrew J.

Missions to Lagrange points are becoming increasingly popular amongst spacecraft mission planners. Lagrange points are locations in space where the gravity force from two bodies, and the centrifugal force acting on a third body, cancel. To date, all spacecraft that have visited a Lagrange point have done so using high-thrust, chemical propulsion. Due to the increasing availability of low-thrust (high efficiency) propulsive devices, and their increasing capability in terms of fuel efficiency and instantaneous thrust, it has now become possible for a spacecraft to reach a Lagrange point orbit without the aid of chemical propellant. While at any given time there are many paths for a low-thrust trajectory to take, only one is optimal. The traditional approach to spacecraft trajectory optimization utilizes some form of gradient-based algorithm. While these algorithms offer numerous advantages, they also have a few significant shortcomings. The three most significant shortcomings are: (1) the fact that an initial guess solution is required to initialize the algorithm, (2) the radius of convergence can be quite small and can allow the algorithm to become trapped in local minima, and (3) gradient information is not always assessable nor always trustworthy for a given problem. To avoid these problems, this dissertation is focused on optimizing a low-thrust transfer trajectory from a geocentric orbit to an Earth-Moon, L1, Lagrange point orbit using the method of Particle Swarm Optimization (PSO). The PSO method is an evolutionary heuristic that was originally written to model birds swarming to locate hidden food sources. This PSO method will enable the exploration of the invariant stable manifold of the target Lagrange point orbit in an effort to optimize the spacecraft's low-thrust trajectory. Examples of these optimized trajectories are presented and contrasted with those found using traditional, gradient-based approaches. In summary, the results of this dissertation find

Orbit Determination (OD) Error Analysis Results for the Triana Sun-Earth L1 Libration Point Mission and for the Fourier Kelvin Stellar Interferometer (FKSI) Sun-Earth L2 Libration Point Mission Concept

NASA Technical Reports Server (NTRS)

Marr, Greg C.

2003-01-01

The Triana spacecraft was designed to be launched by the Space Shuttle. The nominal Triana mission orbit will be a Sun-Earth L1 libration point orbit. Using the NASA Goddard Space Flight Center's Orbit Determination Error Analysis System (ODEAS), orbit determination (OD) error analysis results are presented for all phases of the Triana mission from the first correction maneuver through approximately launch plus 6 months. Results are also presented for the science data collection phase of the Fourier Kelvin Stellar Interferometer Sun-Earth L2 libration point mission concept with momentum unloading thrust perturbations during the tracking arc. The Triana analysis includes extensive analysis of an initial short arc orbit determination solution and results using both Deep Space Network (DSN) and commercial Universal Space Network (USN) statistics. These results could be utilized in support of future Sun-Earth libration point missions.

Binary Colloidal Alloy Test-3 and 4: Critical Point

NASA Technical Reports Server (NTRS)

Weitz, David A.; Lu, Peter J.

2007-01-01

Binary Colloidal Alloy Test - 3 and 4: Critical Point (BCAT-3-4-CP) will determine phase separation rates and add needed points to the phase diagram of a model critical fluid system. Crewmembers photograph samples of polymer and colloidal particles (tiny nanoscale spheres suspended in liquid) that model liquid/gas phase changes. Results will help scientists develop fundamental physics concepts previously cloaked by the effects of gravity.

Optical transmission for the James Webb Space Telescope

NASA Astrophysics Data System (ADS)

Lightsey, Paul A.; Gallagher, Benjamin B.; Nickles, Neal; Copp, Tracy

2012-09-01

The fabrication and coating of the mirrors for the James Webb Space Telescope has been completed. The spectral reflectivity of the protected gold coated beryllium mirrors has been measured. The predicted end-of-life transmission through the telescope builds from these values. The additional phenomena that have been analyzed are contamination effects and effects of the environment for the JWST operation about the Earth-Sun L2 Lagrange libration point. The L2 environment analysis has been based on radiation testing of mirror samples and hypervelocity testing to assess the micrometeoroid impact effects. The mirror showed no change in reflectance over the VIS-SWIR wavelengths after exposure to 6-9 Grad (Si) that simulated 6 years orbiting the L2 Lagrange point. The effects of hypervelocity particle impacts on the mirrors from test data has been extrapolated to the to the anticipated flux characteristics for micrometeoroids at the L2 environment. The results show that the micrometeoroid effects are orders of magnitude below the particulate contamination effects. The final end-of-life transmission for the mirrors including all of these phenomena will meet the performance requirements for JWST.

Formation Flying Satellite Control Around the L2 Sun-Earth Libration Point

NASA Technical Reports Server (NTRS)

Hamilton, Nicholas H.; Folta, David; Carpenter, Russell; Bauer, Frank (Technical Monitor)

2002-01-01

This paper discusses the development of a linear control algorithm for formations in the vicinity of the L2 sun-Earth libration point. The development of a simplified extended Kalman filter is included as well. Simulations are created for the analysis of the stationkeeping and various formation maneuvers of the Stellar Imager mission. The simulations provide tracking error, estimation error, and control effort results. For formation maneuvering, the formation spacecraft track to within 4 meters of their desired position and within 1.5 millimeters per second of their desired zero velocity. The filter, with few exceptions, keeps the estimation errors within their three-sigma values. Without noise, the controller performs extremely well, with the formation spacecraft tracking to within several micrometers. Each spacecraft uses around 1 to 2 grams of propellant per maneuver, depending on the circumstances.

4. WASHBURN POINT VISTA AREA. HALF DOME AT CENTER REAR. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

4. WASHBURN POINT VISTA AREA. HALF DOME AT CENTER REAR. LOOKING NE. GIS: N-37 43 13.7 / W-119 34 23.0 - Glacier Point Road, Between Chinquapin Flat & Glacier Point, Yosemite Village, Mariposa County, CA

Effects of Altering Levothyroxine (L-T4) Doses on Quality of Life, Mood, and Cognition in L-T4 Treated Subjects.

PubMed

Samuels, Mary H; Kolobova, Irina; Niederhausen, Meike; Janowsky, Jeri S; Schuff, Kathryn G

2018-05-01

The brain is a critical target organ for thyroid hormone, but it is unclear whether variations in thyroid function within and near the reference range affect quality of life, mood, or cognition. A total of 138 subjects with levothyroxine (L-T4)-treated hypothyroidism and normal thyrotropin (TSH) levels underwent measures of quality of life (36-Item Short Form Health Survey, Underactive Thyroid-Dependent Quality of Life Questionnaire), mood (Profile of Mood States, Affective Lability Scale), and cognition (executive function, memory). They were then randomly assigned to receive an unchanged, higher, or lower L-T4 dose in double-blind fashion, targeting one of three TSH ranges (0.34 to 2.50, 2.51 to 5.60, or 5.61 to 12.0 mU/L). Doses were adjusted every 6 weeks based on TSH levels. Baseline measures were reassessed at 6 months. At the end of the study, by intention to treat, mean L-T4 doses were 1.50 ± 0.07, 1.32 ± 0.07, and 0.78 ± 0.08 μg/kg (P < 0.001), and mean TSH levels were 1.85 ± 0.25, 3.93 ± 0.38, and 9.49 ± 0.80 mU/L (P < 0.001), respectively, in the three arms. There were minor differences in a few outcomes between the three arms, which were no longer significant after correction for multiple comparisons. Subjects could not ascertain how their L-T4 doses had been adjusted (P = 0.55) but preferred L-T4 doses they perceived to be higher (P < 0.001). Altering L-T4 doses in hypothyroid subjects to vary TSH levels in and near the reference range does not affect quality of life, mood, or cognition. L-T4-treated subjects prefer perceived higher L-T4 doses despite a lack of objective benefit. Adjusting L-T4 doses in hypothyroid patients based on symptoms in these areas may not result in significant clinical improvement.

Orbital Maneuvers for Spacecrafts Travelling to/from the Lagrangian Points

NASA Astrophysics Data System (ADS)

Bertachini, A.

The well-known Lagrangian points that appear in the planar restricted three-body problem (Szebehely, 1967) are very important for astronautical applications. They are five points of equilibrium in the equations of motion, what means that a particle located at one of those points with zero velocity will remain there indefinitely. The collinear points (L1, L2 and L3) are always unstable and the triangular points (L4 and L5) are stable in the present case studied (Sun-Earth system). They are all very good points to locate a space-station, since they require a small amount of V (and fuel), the control to be used for station-keeping. The triangular points are specially good for this purpose, since they are stable equilibrium points. In this paper, the planar restricted three-body problem is regularized (using Lemaître regularization) and combined with numerical integration and gradient methods to solve the two point boundary value problem (the Lambert's three-body problem). This combination is applied to the search of families of transfer orbits between the Lagrangian points and the Earth, in the Sun-Earth system, with the minimum possible cost of the control used. So, the final goal of this paper is to find the magnitude and direction of the two impulses to be applied in the spacecraft to complete the transfer: the first one when leaving/arriving at the Lagrangian point and the second one when arriving/living at the Earth. This paper is a continuation of two previous papers that studied transfers in the Earth-Moon system: Broucke (1979), that studied transfer orbits between the Lagrangian points and the Moon and Prado (1996), that studied transfer orbits between the Lagrangian points and the Earth. So, the equations of motion are: whereis the pseudo-potential given by: To solve the TPBVP in the regularized variables the following steps are used: i) Guess a initial velocity Vi, so together with the initial prescribed position ri the complete initial state is known; ii

A Chang'e-4 mission concept and vision of future Chinese lunar exploration activities

NASA Astrophysics Data System (ADS)

Wang, Qiong; Liu, Jizhong

2016-10-01

A novel concept for Chinese Chang'e-4 lunar exploration mission is presented in this paper at first. After the success of Chang'e-3, its backup probe, Chang'e-4 lander/rover combination, would be upgraded and land on the unexplored lunar farside by the aid of a relay satellite near the second Earth-Moon Lagrange point. Mineralogical and geochemical surveys on the farside to study the formation and evolution of lunar crust and observations at low radio frequencies to track the signals of the Universe's Dark Ages are priorities. Follow-up Chinese lunar exploration activities before 2030 are envisioned as building a robotic lunar science station by three to five missions. Finally several methods of international cooperation are proposed.

Lunar and Lagrangian Point L1 L2 CubeSat Communication and Navigation Considerations

NASA Technical Reports Server (NTRS)

Schaire, Scott; Wong, Yen F.; Altunc, Serhat; Bussey, George; Shelton, Marta; Folta, Dave; Gramling, Cheryl; Celeste, Peter; Anderson, Mile; Perrotto, Trish;

Characterization of the L4-L5-S1 motion segment using the stepwise reduction method.

PubMed

Jaramillo, Héctor Enrique; Puttlitz, Christian M; McGilvray, Kirk; García, José J

2016-05-03

The two aims of this study were to generate data for a more accurate calibration of finite element models including the L5-S1 segment, and to find mechanical differences between the L4-L5 and L5-S1 segments. Then, the range of motion (ROM) and facet forces for the L4-S1 segment were measured using the stepwise reduction method. This consists of sequentially testing and reducing each segment in nine stages by cutting the ligaments, facet capsules, and removing the nucleus. Five L4-S1 human segments (median: 65 years, range: 53-84 years, SD=11.0 years) were loaded under a maximum pure moment of 8Nm. The ROM was measured using stereo-photogrammetry via tracking of three markers and the facet contact forces (CF) were measured using a Tekscan system. The ROM for the L4-L5 segment and all stages showed good agreement with published data. The major differences in ROM between the L4-L5 and L5-S1 segments were found for lateral bending and all stages, for which the L4-L5 ROM was about 1.5-3 times higher than that of the L5-S1 segment, consistent with L5-S1 facet CF about 1.3 to 4 times higher than those measured for the L4-L5 segment. For the other movements and few stages, the L4-L5 ROM was significantly lower that of the L5-S1 segment. ROM and CF provide important baseline data for more accurate calibration of FE models and to understand the role that their structures play in lower lumbar spine mechanics. Copyright © 2016 Elsevier Ltd. All rights reserved.

Factors influencing superimposition error of 3D cephalometric landmarks by plane orientation method using 4 reference points: 4 point superimposition error regression model.

PubMed

Hwang, Jae Joon; Kim, Kee-Deog; Park, Hyok; Park, Chang Seo; Jeong, Ho-Gul

2014-01-01

Superimposition has been used as a method to evaluate the changes of orthodontic or orthopedic treatment in the dental field. With the introduction of cone beam CT (CBCT), evaluating 3 dimensional changes after treatment became possible by superimposition. 4 point plane orientation is one of the simplest ways to achieve superimposition of 3 dimensional images. To find factors influencing superimposition error of cephalometric landmarks by 4 point plane orientation method and to evaluate the reproducibility of cephalometric landmarks for analyzing superimposition error, 20 patients were analyzed who had normal skeletal and occlusal relationship and took CBCT for diagnosis of temporomandibular disorder. The nasion, sella turcica, basion and midpoint between the left and the right most posterior point of the lesser wing of sphenoidal bone were used to define a three-dimensional (3D) anatomical reference co-ordinate system. Another 15 reference cephalometric points were also determined three times in the same image. Reorientation error of each landmark could be explained substantially (23%) by linear regression model, which consists of 3 factors describing position of each landmark towards reference axes and locating error. 4 point plane orientation system may produce an amount of reorientation error that may vary according to the perpendicular distance between the landmark and the x-axis; the reorientation error also increases as the locating error and shift of reference axes viewed from each landmark increases. Therefore, in order to reduce the reorientation error, accuracy of all landmarks including the reference points is important. Construction of the regression model using reference points of greater precision is required for the clinical application of this model.

Moduli of quantum Riemannian geometries on <=4 points

NASA Astrophysics Data System (ADS)

Majid, S.; Raineri, E.

2004-12-01

We classify parallelizable noncommutative manifold structures on finite sets of small size in the general formalism of framed quantum manifolds and vielbeins introduced previously [S. Majid, Commun. Math. Phys. 225, 131 (2002)]. The full moduli space is found for ⩽3 points, and a restricted moduli space for 4 points. Generalized Levi-Cività connections and their curvatures are found for a variety of models including models of a discrete torus. The topological part of the moduli space is found for ⩽9 points based on the known atlas of regular graphs. We also remark on aspects of quantum gravity in this approach.

Lunar and Lagrangian Point L1 L2 CubeSat Communication and Navigation Considerations

NASA Technical Reports Server (NTRS)

Schaire, Scott; Wong, Yen F.; Altunc, Serhat; Bussey, George D.; Shelton, Marta; Folta, Dave; Gramling, Cheryl; Celeste, Peter; Anderson, Mike; Perrotto, Trish;

Imaging Near-Earth Electron Densities Using Thomson Scattering

DTIC Science & Technology

2009-01-15

geocentric solar magnetospheric (GSM) coordinates1. TECs were initially computed from a viewing loca- tion at the Sun-Earth L1 Lagrange point2 for both...further find that an elliptical Earth orbit (apogee ~30 RE) is a suitable lower- cost option for a demonstration mission. 5. SIMULATED OBSERVATIONS We

The James Webb Space Telescope

NASA Technical Reports Server (NTRS)

Mather, John

2003-01-01

The James Webb Space Telescope (JWST) will extend the discoveries of the Hubble Space Telescope by deploying a large cooled infrared telescope at the Sun-Earth Lagrange point L2. It will have a 6 m aperture and three instruments covering the wavelength range from 0.6 to 28 microns.

Three dimensional elements with Lagrange multipliers for the modified couple stress theory

NASA Astrophysics Data System (ADS)

Kwon, Young-Rok; Lee, Byung-Chai

2018-07-01

Three dimensional mixed elements for the modified couple stress theory are proposed. The C1 continuity for the displacement field, which is required because of the curvature term in the variational form of the theory, is satisfied weakly by introducing a supplementary rotation as an independent variable and constraining the relation between the rotation and the displacement with a Lagrange multiplier vector. An additional constraint about the deviatoric curvature is also considered for three dimensional problems. Weak forms with one constraint and two constraints are derived, and four elements satisfying convergence criteria are developed by applying different approximations to each field of independent variables. The elements pass a [InlineEquation not available: see fulltext.] patch test for three dimensional problems. Numerical examples show that the additional constraint could be considered essential for the three dimensional elements, and one of the elements is recommended for practical applications via the comparison of the performances of the elements. In addition, all the proposed elements can represent the size effect well.

A novel l-isoleucine-4′-dioxygenase and l-isoleucine dihydroxylation cascade in Pantoea ananatis

PubMed Central

Smirnov, Sergey V; Sokolov, Pavel M; Kotlyarova, Veronika A; Samsonova, Natalya N; Kodera, Tomohiro; Sugiyama, Masakazu; Torii, Takayoshi; Hibi, Makoto; Shimizu, Sakayu; Yokozeki, Kenzo; Ogawa, Jun

2013-01-01

A unique operon structure has been identified in the genomes of several plant- and insect-associated bacteria. The distinguishing feature of this operon is the presence of tandem hilA and hilB genes encoding dioxygenases belonging to the PF13640 and PF10014 (BsmA) Pfam families, respectively. The genes encoding HilA and HilB from Pantoea ananatis AJ13355 were cloned and expressed in Escherichia coli. The culturing of E. coli cells expressing hilA (E. coli-HilA) or both hilA and hilB (E. coli-HilAB) in the presence of l-isoleucine resulted in the conversion of l-isoleucine into two novel biogenic compounds: l-4′-isoleucine and l-4,4′-dihydroxyisoleucine, respectively. In parallel, two novel enzymatic activities were detected in the crude cell lysates of the E. coli-HilA and E. coli-HilAB strains: l-isoleucine, 2-oxoglutarate: oxygen oxidoreductase (4′-hydroxylating) (HilA) and l-4′-hydroxyisoleucine, 2-oxoglutarate: oxygen oxidoreductase (4-hydroxylating) (HilB), respectively. Two hypotheses regarding the physiological significance of C-4(4′)-hydroxylation of l-isoleucine in bacteria are also discussed. According to first hypothesis, the l-isoleucine dihydroxylation cascade is involved in synthesis of dipeptide antibiotic in P. ananatis. Another unifying hypothesis is that the C-4(4′)-hydroxylation of l-isoleucine in bacteria could result in the synthesis of signal molecules belonging to two classes: 2(5H)-furanones and analogs of N-acyl homoserine lactone. PMID:23554367

Summary and evaluation: fuel dynamics loss-of-flow experiments (tests L2, L3, and L4)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Barts, E.W.; Deitrich, L.W.; Eberhart, J.G.

1975-09-01

Three similar experiments conducted to support the analyses of hypothetical LMFBR unprotected-loss-of-flow accidents are summarized and evaluated. The tests, designated L2, L3, and L4, provided experimental data against which accident-analysis codes could be compared, so as to guide further analysis and modeling of the initiating phases of the hypothetical accident. The tests were conducted using seven-pin bundles of mixed-oxide fuel pins in Mark-II flowing-sodium loops in the TREAT reactor. Test L2 used fresh fuel. Tests L3 and L4 used irradiated fuel pins having, respectively, ''intermediate-power'' (no central void) and ''high-power'' (fully developed central void) microstructure. 12 references. (auth)

Point-spread function reconstruction in ground-based astronomy by l(1)-l(p) model.

PubMed

Chan, Raymond H; Yuan, Xiaoming; Zhang, Wenxing

2012-11-01

In ground-based astronomy, images of objects in outer space are acquired via ground-based telescopes. However, the imaging system is generally interfered by atmospheric turbulence, and hence images so acquired are blurred with unknown point-spread function (PSF). To restore the observed images, the wavefront of light at the telescope's aperture is utilized to derive the PSF. A model with the Tikhonov regularization has been proposed to find the high-resolution phase gradients by solving a least-squares system. Here we propose the l(1)-l(p) (p=1, 2) model for reconstructing the phase gradients. This model can provide sharper edges in the gradients while removing noise. The minimization models can easily be solved by the Douglas-Rachford alternating direction method of a multiplier, and the convergence rate is readily established. Numerical results are given to illustrate that the model can give better phase gradients and hence a more accurate PSF. As a result, the restored images are much more accurate when compared to the traditional Tikhonov regularization model.

L-Cysteine and L-AP4 microinjections in the rat caudal ventrolateral medulla decrease arterial blood pressure.

PubMed

Takemoto, Yumi

2014-12-01

The thiol amino acid L-cysteine increases arterial blood pressure (ABP) when injected into the cerebrospinal fluid space in conscious rats, indicating a pressor response to centrally acting L-cysteine. A prior synaptic membrane binding assay suggests that L-cysteine has a strong affinity for the L-2-amino-4-phosphonobutyric acid (L-AP4) binding site. The central action of L-cysteine may be vial-AP4 sensitive receptors. The present study investigated cardiovascular responses to L-cysteine and L-ap4 microinjected into the autonomic area of the caudal ventrolateral medulla (CVLM) where inhibitory neurons regulate ABP via pre-sympathetic vasomotor neurons. Both the injection of L-cysteine and L-AP4 in the CVLM sites identified with L-glutamate produced the same depressor and bradycardic responses in urethane-anesthetized rats. Neither a prior antagonist microinjection of MK801 for the N-methyl-D-aspartate (NMDA) receptor nor CNQX for the non-NMDA receptor attenuated the responses to L-cysteine, but the combination of the two receptor blocking with an additional prior injection abolished the response. In contrast, either receptor blockade alone abolished the response to L-AP4, indicating distinct mechanisms between responses to L-cysteine and L-AP4 in the CVLM. The results indicate that the CVLM is a central active site for L-cysteine's cardiovascular response. Central L-cysteine's action could be independent of the L-AP4 sensitive receptors. Cardiovascular regulation may involve endogenous L-cysteine in the CVLM. Further multidisciplinary examinations are required to elaborate on L-cysteine's functional roles in the CVLM. Copyright © 2014 Elsevier B.V. All rights reserved.

Superconductivity at 33-37 K in A L n2Fe4As4O2 (A =Kand Cs;L n =lanthanides)

NASA Astrophysics Data System (ADS)

Wu, Si-Qi; Wang, Zhi-Cheng; He, Chao-Yang; Tang, Zhang-Tu; Liu, Yi; Cao, Guang-Han

2017-09-01

We have synthesized ten iron oxyarsenides, K L n2Fe4As4O2 (L n =Gd,Tb,Dy, and Ho) and Cs L n2Fe4As4O2 (L n =Nd,Sm,Gd,Tb,Dy, and Ho) , with the aid of the lattice-match approach. The resultant compounds possess hole-doped conducting double FeAs layers [AFe4As4] 2 - that are separated by the insulating [Ln2O2] 2 + slabs. Measurements of electrical resistivity and dc magnetic susceptibility demonstrate bulk superconductivity at Tc=33 -37 K. We find that Tc correlates with the axial ratio c /a for all 12442-type superconductors discovered. Also, Tc tends to increase with the lattice mismatch, implying that lattice instability plays a role in the enhancement of superconductivity.

Cooperatively surrounding control for multiple Euler-Lagrange systems subjected to uncertain dynamics and input constraints

NASA Astrophysics Data System (ADS)

Chen, Liang-Ming; Lv, Yue-Yong; Li, Chuan-Jiang; Ma, Guang-Fu

2016-12-01

In this paper, we investigate cooperatively surrounding control (CSC) of multi-agent systems modeled by Euler-Lagrange (EL) equations under a directed graph. With the consideration of the uncertain dynamics in an EL system, a backstepping CSC algorithm combined with neural-networks is proposed first such that the agents can move cooperatively to surround the stationary target. Then, a command filtered backstepping CSC algorithm is further proposed to deal with the constraints on control input and the absence of neighbors’ velocity information. Numerical examples of eight satellites surrounding one space target illustrate the effectiveness of the theoretical results. Project supported by the National Basic Research Program of China (Grant No. 2012CB720000) and the National Natural Science Foundation of China (Grant Nos. 61304005 and 61403103).

Searching for life with the Terrestrial Planet Finder: Lagrange point options for a formation flying interferometer

NASA Technical Reports Server (NTRS)

Beichman, C.; Gomez, G.; Lo, M.; Masdemont, J.; Romans, L.

2002-01-01

In this paper, we describe the mission design for TPF assuming a distributed spacecraft concept using formation flight around both a halo orbit around L2 as well as a heliocentric orbit. Although the mission architecture is still under study, the next two years will include study of four design cncepts and a downselect to two concepts around 2005.

78 FR 69516 - DeltaPoint Capital IV, L.P.; License No. 02/02-0662; Notice Seeking Exemption Under the Small...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-11-19

... 107.730). DeltaPoint Capital IV, L.P. provided financing to BioMaxx, Inc., 1 Fishers Road, Suite 160...., an Associate of DeltaPoint Capital IV, L.P., owns more than ten percent of BioMaxx, Inc. Therefore...

Absolute calibration accuracy of L4 TM and L5 TM sensor image pairs

USGS Publications Warehouse

Chander, G.; Micijevic, E.

2006-01-01

The Landsat suite of satellites has collected the longest continuous archive of multispectral data of any land-observing space program. From the Landsat program's inception in 1972 to the present, the Earth science user community has benefited from a historical record of remotely sensed data. However, little attention has been paid to ensuring that the data are calibrated and comparable from mission to mission, Launched in 1982 and 1984 respectively, the Landsat 4 (L4) and Landsat 5 (L5) Thematic Mappers (TM) are the backbone of an extensive archive of moderate resolution Earth imagery. To evaluate the "current" absolute accuracy of these two sensors, image pairs from the L5 TM and L4 TM sensors were compared. The approach involves comparing image statistics derived from large common areas observed eight days apart by the two sensors. The average percent differences in reflectance estimates obtained from the L4 TM agree with those from the L5 TM to within 15 percent. Additional work to characterize the absolute differences between the two sensors over the entire mission is in progress.

Distributed-Lagrange-Multiplier-based computational method for particulate flow with collisions

NASA Astrophysics Data System (ADS)

Ardekani, Arezoo; Rangel, Roger

2006-11-01

A Distributed-Lagrange-Multiplier-based computational method is developed for colliding particles in a solid-fluid system. A numerical simulation is conducted in two dimensions using the finite volume method. The entire domain is treated as a fluid but the fluid in the particle domains satisfies a rigidity constraint. We present an efficient method for predicting the collision between particles. In earlier methods, a repulsive force was applied to the particles when their distance was less than a critical value. In this method, an impulsive force is computed. During the frictionless collision process between two particles, linear momentum is conserved while the tangential forces are zero. Thus, instead of satisfying a condition of rigid body motion for each particle separately, as done when particles are not in contact, both particles are rigidified together along their line of centers. Particles separate from each other when the impulsive force is less than zero and after this time, a rigidity constraint is satisfied for each particle separately. Grid independency is implemented to ensure the accuracy of the numerical simulation. A comparison between this method and previous collision strategies is presented and discussed.

Non-collinear libration points in ER3BP with albedo effect and oblateness

NASA Astrophysics Data System (ADS)

Idrisi, M. Javed; Ullah, M. Shahbaz

2018-06-01

In this paper we establish a relation between direct radiations (generally called radiation factor) and reflected radiations (albedo) to show their effects on the existence and stability of non-collinear libration points in the elliptic restricted three-body problem taking into account the oblateness of smaller primary. It is discussed briefly when α =0 and σ =0, the non-collinear libration points form an isosceles triangle with the primaries and as e increases the libration points L_{4,5} move vertically downward (α , σ and e represents the radiation factor, oblateness factor and eccentricity of the primaries respectively). If α = 0 but σ ≠ 0, the libration points slightly displaced to the right-side from its previous location and form scalene triangle with the primaries and go vertically downward as e increases. If α ≠ 0 and σ ≠ 0, the libration points L_{4,5} form scalene triangle with the primaries and as e increases L_{4,5} move downward and displaced to the left-side. Also, the libration points L_{4,5} are stable for the critical mass parameter μ ≤ μ c.

Laboratory-based performance evaluation of PIMA CD4+ T-lymphocyte count point-of-care by lay-counselors in Kenya.

PubMed

Zeh, Clement; Rose, Charles E; Inzaule, Seth; Desai, Mitesh A; Otieno, Fredrick; Humwa, Felix; Akoth, Benta; Omolo, Paul; Chen, Robert T; Kebede, Yenew; Samandari, Taraz

2017-09-01

CD4+ T-lymphocyte count testing at the point-of-care (POC) may improve linkage to care of persons diagnosed with HIV-1 infection, but the accuracy of POC devices when operated by lay-counselors in the era of task-shifting is unknown. We examined the accuracy of Alere's Pima™ POC device on both capillary and venous blood when performed by lay-counselors and laboratory technicians. In Phase I, we compared the perfomance of POC against FACSCalibur™ for 280 venous specimens by laboratory technicians. In Phase II we compared POC performance by lay-counselors versus laboratory technicians using 147 paired capillary and venous specimens, and compared these to FACSCalibur™. Statistical analyses included Bland-Altman analyses, concordance correlation coefficient, sensitivity, and specificity at treatment eligibility thresholds of 200, 350, and 500cells/μl. Phase I: POC sensitivity and specificity were 93.0% and 84.1% at 500cells/μl, respectively. Phase II: Good agreement was observed for venous POC results from both lay-counselors (concordance correlation coefficient (CCC)=0.873, bias -86.4cells/μl) and laboratory technicians (CCC=0.920, bias -65.7cells/μl). Capillary POC had good correlation: lay-counselors (CCC=0.902, bias -71.2cells/μl), laboratory technicians (CCC=0.918, bias -63.0cells/μl). Misclassification at the 500 cells/μl threshold for venous blood was 13.6% and 10.2% for lay-counselors and laboratory technicians and 12.2% for capillary blood in both groups. POC tended to under-classify the CD4 values with increasingly negative bias at higher CD4 values. Pima™ results were comparable to FACSCalibur™ for both venous and capillary specimens when operated by lay-counselors. POC CD4 testing has the potential to improve linkage to HIV care without burdening laboratory technicians in resource-limited settings. Published by Elsevier B.V.

Magnitudes, nature, and effects of point and nonpoint discharges in the Chattahoochee River Basin, Atlanta to West Point Dam, Georgia

USGS Publications Warehouse

Stamer, J.K.; Cherry, Rodney N.; Faye, R.E.; Kleckner, R.L.

1979-01-01

During the period April 1975 to June 1978, the U.S. Geological Survey conducted a river-quality assessment of the Upper Chattahoochee River basin in Georgia. One objective of the study was to assess the magnitudes, nature, and effects of point and non-point discharges in the Chattahoochee River basin from Atlanta to the West Point Dam. On an average annual basis and during the storm period of March 1215, 1976, non-point-source loads for most constituents analyzed were larger than point-source loads at the Whitesburg station, located on the Chattahoochee River about 40 river miles downstream of Atlanta. Most of the non-point-source constituent loads in the Atlanta-to-Whitesburg reach were from urban areas. Average annual point-source discharges accounted for about 50 percent of the dissolved nitrogen, total nitrogen, and total phosphorus loads, and about 70 percent of the dissolved phosphorus loads at Whitesburg. During weekends, power generation at the upstream Buford Dam hydroelectric facility is minimal. Streamflow at the Atlanta station during dry-weather weekends is estimated to be about 1,200 ft3/s (cubic feet per second). Average daily dissolved-oxygen concentrations of less than 5.0 mg/L (milligrams per liter) occurred often in the river, about 20 river miles downstream from Atlanta during these periods from May to November. During a low-flow period, June 1-2, 1977, five municipal point sources contributed 63 percent of the ultimate biochemical oxygen demand, 97 percent of the ammonium nitrogen, 78 percent of the total nitrogen, and 90 percent of the total phosphorus loads at the Franklin station, at the upstream end of West Point Lake. Average daily concentrations of 13 mg/L of ultimate biochemical oxygen demand and 1.8 mg/L of ammonium nitrogen were observed about 2 river miles downstream from two of the municipal point sources. Carbonaceous and nitrogenous oxygen demands caused dissolved-oxygen concentrations between 4.1 and 5.0 mg/L to occur in a 22-mile

Testing of Lagrange multiplier damped least-squares control algorithm for woofer-tweeter adaptive optics

PubMed Central

Zou, Weiyao; Burns, Stephen A.

2012-01-01

A Lagrange multiplier-based damped least-squares control algorithm for woofer-tweeter (W-T) dual deformable-mirror (DM) adaptive optics (AO) is tested with a breadboard system. We show that the algorithm can complementarily command the two DMs to correct wavefront aberrations within a single optimization process: the woofer DM correcting the high-stroke, low-order aberrations, and the tweeter DM correcting the low-stroke, high-order aberrations. The optimal damping factor for a DM is found to be the median of the eigenvalue spectrum of the influence matrix of that DM. Wavefront control accuracy is maximized with the optimized control parameters. For the breadboard system, the residual wavefront error can be controlled to the precision of 0.03 μm in root mean square. The W-T dual-DM AO has applications in both ophthalmology and astronomy. PMID:22441462

Long Term Missions at the Sun-Earth Libration Point L1: ACE, SOHO, and WIND

NASA Technical Reports Server (NTRS)

Roberts, Craig E.

2011-01-01

Three heliophysics missions - the Solar Heliospheric Observatory (SOHO), the Advanced Composition Explorer (ACE), and the Global Geoscience WIND - have been orbiting the Sun-Earth interior libration point L1 continuously since 1996, 1997, and 2004, respectively. ACE and WIND (both NASA missions) and SOHO (an ESA-NASA joint mission) are all operated from the NASA Goddard Space Flight Center Flight Dynamics Facility. While ACE and SOHO have been dedicated libration point orbiters since their launches, WIND prior to 2004 flew a remarkable 10-year deep-space trajectory that featured 38 targeted lunar flybys. The L1 orbits and the mission histories of the three spacecraft are briefly reviewed, and the station-keeping techniques and orbit maneuver experience are discussed.

Long Term Missions at the Sun-Earth Libration Point L1: ACE, SOHO, and WIND

NASA Technical Reports Server (NTRS)

Roberts, Craig E.

2011-01-01

Three heliophysics missions -- the Advanced Composition Explorer (ACE), Solar Heliospheric Observatory (SOHO), and the Global Geoscience WIND -- have been orbiting the Sun-Earth interior libration point L1 continuously since 1997, 1996, and 2004, respectively. ACE and WIND (both NASA missions) and SOHO (an ESA-NASA joint mission) are all operated from the NASA Goddard Space Flight Center (GSFC). While ACE and SOHO have been dedicated libration point orbiters since their launches, WIND has had also a remarkable 10-year career flying a deep-space, multiple lunar-flyby trajectory prior to 2004. That era featured 36 targeted lunar flybys with excursions to both L1 and L2 before its final insertion in L1 orbit. A figure depicts the orbits of the three spacecraft, showing projections of the orbits onto the orthographic planes of a solar rotating ecliptic frame of reference. The SOHO orbit is a quasi-periodic halo orbit, where the frequencies of the in-plane and out-of-plane motions are practically equal. Such an orbit is seen to repeat itself with a period of approximately 178 days. For ACE and WIND, the frequencies of the in-plane and out-of-plane motions are unequal, giving rise to the characteristic Lissajous motion. ACE's orbit is of moderately small amplitude, whereas WIND's orbit is a large-amplitude Lissajous of dimensions close to those of the SOHO halo orbit. As motion about the collinear points is inherently unstable, stationkeeping maneuvers are necessary to prevent orbital decay and eventual escape from the L1 region. Though the three spacecraft are dissimilar (SOHO is a 3-axis stabilized Sun pointer, WIND is a spin-stabilized ecliptic pole pointer, and ACE is also spin-stabilized with its spin axis maintained between 4 and 20 degrees of the Sun), the stationkeeping technique for the three is fundamentally the same. The technique consists of correcting the energy of the orbit via a delta-V directed parallel or anti-parallel to the Spacecraft-to-Sun line. SOHO

PF-4/CXCL4 and CXCL4L1 exhibit distinct subcellular localization and a differentially regulated mechanism of secretion.

PubMed

Lasagni, Laura; Grepin, Renaud; Mazzinghi, Benedetta; Lazzeri, Elena; Meini, Claudia; Sagrinati, Costanza; Liotta, Francesco; Frosali, Francesca; Ronconi, Elisa; Alain-Courtois, Nathalie; Ballerini, Lara; Netti, Giuseppe Stefano; Maggi, Enrico; Annunziato, Francesco; Serio, Mario; Romagnani, Sergio; Bikfalvi, Andreas; Romagnani, Paola

2007-05-15

PF-4/CXCL4 is a member of the CXC chemokine family, which is mainly produced by platelets and known for its pleiotropic biological functions. Recently, the proteic product of a nonallelic variant gene of CXCL4 was isolated from human platelets and named as CXCL4L1. CXCL4L1 shows only 4.3% amino acid divergence in the mature protein, but exhibits a 38% amino acid divergence in the signal peptide region. We hypothesized that this may imply a difference in the cell type in which CXCL4L1 is expressed or a difference in its mode of secretion. In different types of transfected cells, CXCL4 and CXCL4L1 exhibited a distinct subcellular localization and a differential regulation of secretion, CXCL4 being stored in secretory granules and released in response to protein kinase C activation, whereas CXCL4L1 was continuously synthesized and secreted through a constitutive pathway. A protein kinase C-regulated CXCL4 secretion was observed also in lymphocytes, a cell type expressing mainly CXCL4 mRNA, whereas smooth muscle cells, which preferentially expressed CXCL4L1, exhibited a constitutive pathway of secretion. These results demonstrate that CXCL4 and CXCL4L1 exhibit a distinct subcellular localization and are secreted in a differentially regulated manner, suggesting distinct roles in inflammatory or homeostatic processes.

Guidance of a Solar Sail Spacecraft to the Sun - L(2) Point.

NASA Astrophysics Data System (ADS)

Hur, Sun Hae

The guidance of a solar sail spacecraft along a minimum-time path from an Earth orbit to a region near the Sun-Earth L_2 libration point is investigated. Possible missions to this point include a spacecraft "listening" for possible extra-terrestrial electromagnetic signals and a science payload to study the geomagnetic tail. A key advantage of the solar sail is that it requires no fuel. The control variables are the sail angles relative to the Sun-Earth line. The thrust is very small, on the order of 1 mm/s^2, and its magnitude and direction are highly coupled. Despite this limited controllability, the "free" thrust can be used for a wide variety of terminal conditions including halo orbits. If the Moon's mass is lumped with the Earth, there are quasi-equilibrium points near L_2. However, they are unstable so that some form of station keeping is required, and the sail can provide this without any fuel usage. In the two-dimensional case, regulating about a nominal orbit is shown to require less control and result in smaller amplitude error response than regulating about a quasi-equilibrium point. In the three-dimensional halo orbit case, station keeping using periodically varying gains is demonstrated. To compute the minimum-time path, the trajectory is divided into two segments: the spiral segment and the transition segment. The spiral segment is computed using a control law that maximizes the rate of energy increase at each time. The transition segment is computed as the solution of the time-optimal control problem from the endpoint of the spiral to the terminal point. It is shown that the path resulting from this approximate strategy is very close to the exact optimal path. For the guidance problem, the approximate strategy in the spiral segment already gives a nonlinear full-state feedback law. However, for large perturbations, follower guidance using an auxiliary propulsion is used for part of the spiral. In the transition segment, neighboring extremal feedback

Advances in Highly Constrained Multi-Phase Trajectory Generation using the General Pseudospectral Optimization Software (GPOPS)

DTIC Science & Technology

2013-08-01

release; distribution unlimited. PA Number 412-TW-PA-13395 f generic function g acceleration due to gravity h altitude L aerodynamic lift force L Lagrange...cost m vehicle mass M Mach number n number of coefficients in polynomial regression p highest order of polynomial regression Q dynamic pressure R...Method (RPM); the collocation points are defined by the roots of Legendre -Gauss- Radau (LGR) functions.9 GPOPS also automatically refines the “mesh” by

Synthesis and the crystal and molecular structures of (H{sub 3}L . Cl)[CoCl{sub 4}] and H{sub 2}L[CuBr{sub 4}] (L is 2,4,6-Tri(N,N-dimethylamino)methylphenol)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kovalchukova, O. V., E-mail: okovalchukova@mail.ru; Stash, A. I.; Strashnova, S. B.

2010-05-15

The complex compounds (H{sub 3}L . Cl)[CoCl{sub 4}] (I) and H{sub 2}L[CuBr{sub 4}] (II), where L is 2,4,6-tri(N,N-dimethylamino)methylphenol, were isolated in the crystalline state and studied by X-ray diffraction. The organic cations were found to be outer-sphere ligands. All three nitrogen atoms of the tertiary amino groups are protonated. In compound I, the H{sub 3}L{sup 3+} cation exists as the cis tautomer. In compound II, the H{sub 2}L{sup 2+} dication exists as the trans isomer. In the crystal structure, the dications are arranged in layers via hydrogen bonds.

Diastereoselective synthesis of L: -threo-3,4-dihydroxyphenylserine by low-specific L: -threonine aldolase mutants.

PubMed

Gwon, Hui-Jeong; Baik, Sang-Ho

2010-01-01

Diastereoselectivity-enhanced mutants of L: -threonine aldolase (L: -TA) for L: -threo-3,4-dihydroxyphenylserine (L: -threo-DOPS) synthesis were isolated by error-prone PCR followed by a high-throughput screening. The most improved mutant was achieved from the mutant T3-3mm2, showing a 4-fold increase over the wild-type L: -TA. When aldol condensation activity was examined using whole cells of T3-3mm2, its de was constantly maintained at 55% during the batch reactions for 80 h, yielding 3.8 mg L: -threo-DOPS/ml.

Group for High Resolution Sea Surface Temperature (GHRSST) analysis fields inter-comparisons—Part 2: Near real time web-based level 4 SST Quality Monitor (L4-SQUAM)

NASA Astrophysics Data System (ADS)

Dash, Prasanjit; Ignatov, Alexander; Martin, Matthew; Donlon, Craig; Brasnett, Bruce; Reynolds, Richard W.; Banzon, Viva; Beggs, Helen; Cayula, Jean-Francois; Chao, Yi; Grumbine, Robert; Maturi, Eileen; Harris, Andy; Mittaz, Jonathan; Sapper, John; Chin, Toshio M.; Vazquez-Cuervo, Jorge; Armstrong, Edward M.; Gentemann, Chelle; Cummings, James; Piollé, Jean-François; Autret, Emmanuelle; Roberts-Jones, Jonah; Ishizaki, Shiro; Høyer, Jacob L.; Poulter, Dave

2012-11-01

There are a growing number of level 4 (L4; gap-free gridded) sea surface temperature (SST) products generated by blending SST data from various sources which are available for use in a wide variety of operational and scientific applications. In most cases, each product has been developed for a specific user community with specific requirements guiding the design of the product. Consequently differences between products are implicit. In addition, anomalous atmospheric conditions, satellite operations and production anomalies may occur which can introduce additional differences. This paper describes a new web-based system called the L4 SST Quality Monitor (L4-SQUAM) developed to monitor the quality of L4 SST products. L4-SQUAM intercompares thirteen L4 products with 1-day latency in an operational environment serving the needs of both L4 SST product users and producers. Relative differences between products are computed and visualized using maps, histograms, time series plots and Hovmöller diagrams, for all combinations of products. In addition, products are compared to quality controlled in situ SST data (available from the in situ SST Quality Monitor, iQUAM, companion system) in a consistent manner. A full history of products statistics is retained in L4-SQUAM for time series analysis. L4-SQUAM complements the two other Group for High Resolution SST (GHRSST) tools, the GHRSST Multi Product Ensemble (GMPE) and the High Resolution Diagnostic Data Set (HRDDS) systems, documented in part 1 of this paper and elsewhere, respectively. Our results reveal significant differences between SST products in coastal and open ocean areas. Differences of >2 °C are often observed at high latitudes partly due to different treatment of the sea-ice transition zone. Thus when an ice flag is available, the intercomparisons are performed in two ways: including and excluding ice-flagged grid points. Such differences are significant and call for a community effort to understand their root

North American CO2 fluxes for 2007-2015 from NOAA's CarbonTracker-Lagrange Regional Inverse Modeling Framework

NASA Astrophysics Data System (ADS)

Andrews, A. E.; Hu, L.; Thoning, K. W.; Nehrkorn, T.; Mountain, M. E.; Jacobson, A. R.; Michalak, A.; Dlugokencky, E. J.; Sweeney, C.; Worthy, D. E. J.; Miller, J. B.; Fischer, M. L.; Biraud, S.; van der Velde, I. R.; Basu, S.; Tans, P. P.

2017-12-01

CarbonTracker-Lagrange (CT-L) is a new high-resolution regional inverse modeling system for improved estimation of North American CO2 fluxes. CT-L uses footprints from the Stochastic Time-Inverted Lagrangian Transport (STILT) model driven by high-resolution (10 to 30 km) meteorological fields from the Weather Research and Forecasting (WRF) model. We performed a suite of synthetic-data experiments to evaluate a variety of inversion configurations, including (1) solving for scaling factors to an a priori flux versus additive corrections, (2) solving for fluxes at 3-hrly resolution versus at coarser temporal resolution, (3) solving for fluxes at 1o × 1o resolution versus at large eco-regional scales. Our framework explicitly and objectively solves for the optimal solution with a full error covariance matrix with maximum likelihood estimation, thereby enabling rigorous uncertainty estimates for the derived fluxes. In the synthetic-data inversions, we find that solving for weekly scaling factors of a priori Net Ecosystem Exchange (NEE) at 1o × 1o resolution with optimization of diurnal cycles of CO2 fluxes yields faithful retrieval of the specified "true" fluxes as those solved at 3-hrly resolution. In contrast, a scheme that does not allow for optimization of diurnal cycles of CO2 fluxes suffered from larger aggregation errors. We then applied the optimal inversion setup to estimate North American fluxes for 2007-2015 using real atmospheric CO2 observations, multiple prior estimates of NEE, and multiple boundary values estimated from the NOAA's global Eulerian CarbonTracker (CarbonTracker) and from an empirical approach. Our derived North American land CO2 fluxes show larger seasonal amplitude than those estimated from the CarbonTracker, removing seasonal biases in the CarbonTracker's simulated CO2 mole fractions. Independent evaluations using in-situ CO2 eddy covariance flux measurements and independent aircraft profiles also suggest an improved estimation on North

2 L PEG plus ascorbic acid versus 4 L PEG plus simethicon for colonoscopy preparation: a randomized single-blind clinical trial.

PubMed

Gentile, Maurizio; De Rosa, Michele; Cestaro, Giovanni; Forestieri, Pietro

2013-06-01

The 2 L polyethylene glycol (PEG) lavage solution has been proved to be similarly safe and effective as 4 L PEG formulations, in spite of the reduced volume. To compare low-volume PEG-based solution combined with ascorbic acid with high-volume PEG-based solution combined with simethicon in terms of efficacy and patient tolerability. This was a single-blind prospective randomized trial. Patients were randomized to receive either 2 L PEG plus ascorbic acid (PEG+Asc) or 4 L PEG plus simethicon (PEG+Sim). The primary endpoint was overall colon cleansing evaluation, assessed by blinded investigators using Aronchick score. Secondary end points included patient compliance and tolerability and adverse events. Sixty patients received PEG+Asc and 60 received PEG+Sim. Overall bowel cleansing score was considered adequate in 81.67% of the PEG+Asc and 80% of the PEG+Sim groups, respectively. Excellent and good ratings were recorded in 11.6% and 38.3% receiving PEG+Asc as compared with 26.6% and 23.3% of patients receiving PEG+Sim. Patient tolerability and safety were similar with both the preparations. According to our data, low-volume PEG+Asc has comparable efficacy, safety, and tolerability as high-volume PEG+Sim; therefore, it can be considered as a good alternative solution for bowel preparation. More improvements are necessary to achieve the target of a perfect preparation.

Evaluation of the Consistency among In Situ and Remote Sensing Measurements of CO2 over North America using the CarbonTracker-Lagrange Regional Inverse Modeling Framework

NASA Astrophysics Data System (ADS)

Andrews, A. E.; Trudeau, M.; Hu, L.; Thoning, K. W.; Shiga, Y. P.; Michalak, A. M.; Benmergui, J. S.; Mountain, M. E.; Nehrkorn, T.; O'Dell, C.; Jacobson, A. R.; Miller, J.; Sweeney, C.; Chen, H.; Ploeger, F.; Tans, P. P.

2017-12-01

CarbonTracker-Lagrange (CT-L) is a regional inverse modeling system for estimating CO2 fluxes with rigorous uncertainty quantification. CT-L uses footprints from the Stochastic Time-Inverted Lagrangian Transport (STILT) model driven by high-resolution (10 to 30 km) meteorological fields from the Weather Research and Forecasting (WRF) model. We have computed a library of footprints corresponding to in situ and remote sensing measurements of CO2 over North America for 2007-2015. GOSAT and OCO-2 XCO2 retrievals are simulated using a suite of CT-L terrestrial ecosystem flux estimates that have been optimized with respect to in situ atmospheric CO2 measurements along with fossil fuel fluxes from emissions inventories. A vertical profile of STILT-WRF footprints was constructed corresponding to each simulated satellite retrieval, and CO2 profiles are generated by convolving the footprints with fluxes and attaching initial values advected from the domain boundaries. The stratospheric contribution to XCO2 has been estimated using 4-dimensional CO2 fields from the NOAA CarbonTracker model (version CT2016) and from the Chemical Lagrangian Model of the Stratosphere (CLaMS), after scaling the model fields to match data from the NOAA AirCore surface-to-stratosphere air sampling system. Tropospheric lateral boundary conditions are from CT2016 and from an empirical boundary value product derived from aircraft and marine boundary layer data. The averaging kernel and a priori CO2 profile are taken into account for direct comparisons with retrievals. We have focused on North America due to the relatively dense in situ measurements available with the aim of developing strategies for combined assimilation of in situ and remote sensing data. We will consider the extent to which interannual variability in terrestrial fluxes is manifest in the real and simulated satellite retrievals, and we will investigate possible systematic biases in the satellite retrievals and in the model.

Polestitters: Using Solar Sails for Constant Real-time Sensing of Earth's Polar Regions

NASA Astrophysics Data System (ADS)

Mulligan, P.; Diedrich, B. L.; Barnes, N.; Derbes, B.

2012-12-01

NASA has funded the Sunjammer mission - a near term demonstration of solar sail technology (2014/15). Sunjammer has the potential to demonstrate stationkeeping out of Earth's orbital plane. This is a first step in achieving "polesitter" orbits with year-round, real-time visibility of Earth's polar regions. Potential applications for such missions are illustrated. Solar sails have long been a concept for spacecraft propulsion that works by exchanging momentum with sunlight reflected by large, lightweight, mirrored sails. In addition to enabling propellantless propulsion throughout the solar system and beyond, their continuous thrust enables artificial Lagrange orbits (ALOs), some of which can be called "polesitter" orbits, with 24-hour, year-round visibility of Earth's polar regions. Several potential Earth remote sensing applications have been identified that address the limited temporal and spatial coverage from traditional polar and geostationary satellites. The Galileo spacecraft during its 1990 Earth flyby acquired imagery and radiometer data similar to the view from a polesitter. The Galileo imagery was used to derive aerosols and cloud variations used in atmospheric motion vector (AMV) derivations. Composites of satellite imagery over the South Pole is routinely used to derive atmospheric motion vectors like those performed regularly from geostationary satellites. The JAXA IKAROS mission flew a 14x14m solar sail past Venus in 2010. Sunjammer will demonstrate a state of the art 38x38m solar sail from Earth to an artificial Lagrange orbit located sunward and north of the sun-Earth L1 point. Traditional spacecraft can orbit naturally occurring Lagrange equilibrium points between the sun and Earth. The low, continuous thrust of solar sails can change where these points occur, creating new orbits with a variety of potential applications including polar remote sensing, space weather monitoring, and polar communications. This figure illustrates a selection of

Testing of Lagrange multiplier damped least-squares control algorithm for woofer-tweeter adaptive optics.

PubMed

Zou, Weiyao; Burns, Stephen A

2012-03-20

A Lagrange multiplier-based damped least-squares control algorithm for woofer-tweeter (W-T) dual deformable-mirror (DM) adaptive optics (AO) is tested with a breadboard system. We show that the algorithm can complementarily command the two DMs to correct wavefront aberrations within a single optimization process: the woofer DM correcting the high-stroke, low-order aberrations, and the tweeter DM correcting the low-stroke, high-order aberrations. The optimal damping factor for a DM is found to be the median of the eigenvalue spectrum of the influence matrix of that DM. Wavefront control accuracy is maximized with the optimized control parameters. For the breadboard system, the residual wavefront error can be controlled to the precision of 0.03 μm in root mean square. The W-T dual-DM AO has applications in both ophthalmology and astronomy. © 2012 Optical Society of America

Evidence-Based Leadership Development: The 4L Framework

ERIC Educational Resources Information Center

Scott, Shelleyann; Webber, Charles F.

2008-01-01

Purpose: This paper aims to use the results of three research initiatives to present the life-long learning leader 4L framework, a model for leadership development intended for use by designers and providers of leadership development programming. Design/methodology/approach: The 4L model is a conceptual framework that emerged from the analysis of…

Synthesis and Explosion Hazards of 4-Azido-l-phenylalanine.

PubMed

Richardson, Mark B; Brown, Derek B; Vasquez, Carlos A; Ziller, Joseph W; Johnston, Kevin M; Weiss, Gregory A

2018-04-20

A reliable, scalable, cost-effective, and chromatography-free synthesis of 4-azido-l-phenylalanine beginning from l-phenylalanine is described. Investigations into the safety of the synthesis reveal that the Ullman-like Cu(I)-catalyzed azidation step does not represent a significant risk. The isolated 4-azido-l-phenylalanine product, however, exhibits previously undocumented explosive characteristics.

L-arginine supplementation enhances exhaled NO, breath condensate VEGF, and headache at 4,342 m.

PubMed

Mansoor, Jim K; Morrissey, Brian M; Walby, William F; Yoneda, Ken Y; Juarez, Maya; Kajekar, Radhika; Severinghaus, John W; Eldridge, Marlowe W; Schelegle, Edward S

2005-01-01

We examined the effect of dietary supplementation with L-arginine on breath condensate VEGF, exhaled nitric oxide (NO), plasma erythropoietin, symptoms of acute mountain sickness, and respiratory related sensations at 4,342 m through the course of 24 h in seven healthy male subjects. Serum L-arginine levels increased in treated subjects at time 0, 8, and 24 h compared with placebo, indicating the effectiveness of our treatment. L-arginine had no significant effect on overall Lake Louise scores compared with placebo. However, there was a significant increase in headache within the L-arginine treatment group at 12 h compared with time 0, a change not seen in the placebo condition between these two time points. There was a trend (p = 0.087) toward greater exhaled NO and significant increases in breath condensate VEGF with L-arginine treatment, but no L-arginine effect on serum EPO. These results suggest that L-arginine supplementation increases HIF-1 stabilization in the lung, possibly through a NO-dependent pathway. In total, our observations indicate that L-arginine supplementation is not beneficial in the prophylactic treatment of AMS.

CXCL4 and CXCL4L1 Differentially Affect Monocyte Survival and Dendritic Cell Differentiation and Phagocytosis

PubMed Central

Gouwy, Mieke; Ruytinx, Pieter; Radice, Egle; Claudi, Federico; Van Raemdonck, Katrien; Bonecchi, Raffaella; Locati, Massimo; Struyf, Sofie

2016-01-01

Upon inflammation, circulating monocytes leave the bloodstream and migrate into the tissues, where they differentiate after exposure to various growth factors, cytokines or infectious agents. The best defined macrophage polarization types are M1 and M2. However, the platelet-derived CXC chemokine CXCL4 induces the polarization of macrophages into a unique phenotype. In this study, we compared the effect of CXCL4 and its variant CXCL4L1 on the differentiation of monocytes into macrophages and into immature monocyte-derived dendritic cells (iMDDC). Differently to M-CSF and CXCL4, CXCL4L1 is not a survival factor for monocytes. Moreover, the expression of the chemokine receptors CCR2, CCR5 and CXCR3 was significantly higher on CXCL4L1-treated monocytes compared to M-CSF- and CXCL4-stimulated monocytes. IL-1 receptor antagonist (IL-1RN) expression was upregulated by CXCL4 and downregulated by CXCL4L1, respectively, whereas both chemokines reduced the expression of the mannose receptor (MRC). Furthermore, through activation of CXCR3, CXCL4L1-stimulated monocytes released significantly higher amounts of CCL2 and CXCL8 compared to CXCL4-treated monocytes, indicating more pronounced inflammatory traits for CXCL4L1. In contrast, in CXCL4L1-treated monocytes, the production of CCL22 was lower. Compared to iMDDC generated in the presence of CXCL4L1, CXCL4-treated iMDDC showed an enhanced phagocytic capacity and downregulation of expression of certain surface markers (e.g. CD1a) and specific enzymes (e.g. MMP-9 and MMP-12). CXCL4 and CXCL4L1 did not affect the chemokine receptor expression on iMDDC and cytokine production (CCL2, CCL18, CCL22, CXCL8, IL-10) by CXCL4- or CXCL4L1-differentiated iMDDC was similar. We can conclude that both CXCL4 and CXCL4L1 exert a direct effect on monocytes and iMDDC. However, the resulting phenotypes are different, which suggests a unique role for the two CXCL4 variants in physiology and/or pathology. PMID:27828999

CXCL4 and CXCL4L1 Differentially Affect Monocyte Survival and Dendritic Cell Differentiation and Phagocytosis.

PubMed

Gouwy, Mieke; Ruytinx, Pieter; Radice, Egle; Claudi, Federico; Van Raemdonck, Katrien; Bonecchi, Raffaella; Locati, Massimo; Struyf, Sofie

2016-01-01

Upon inflammation, circulating monocytes leave the bloodstream and migrate into the tissues, where they differentiate after exposure to various growth factors, cytokines or infectious agents. The best defined macrophage polarization types are M1 and M2. However, the platelet-derived CXC chemokine CXCL4 induces the polarization of macrophages into a unique phenotype. In this study, we compared the effect of CXCL4 and its variant CXCL4L1 on the differentiation of monocytes into macrophages and into immature monocyte-derived dendritic cells (iMDDC). Differently to M-CSF and CXCL4, CXCL4L1 is not a survival factor for monocytes. Moreover, the expression of the chemokine receptors CCR2, CCR5 and CXCR3 was significantly higher on CXCL4L1-treated monocytes compared to M-CSF- and CXCL4-stimulated monocytes. IL-1 receptor antagonist (IL-1RN) expression was upregulated by CXCL4 and downregulated by CXCL4L1, respectively, whereas both chemokines reduced the expression of the mannose receptor (MRC). Furthermore, through activation of CXCR3, CXCL4L1-stimulated monocytes released significantly higher amounts of CCL2 and CXCL8 compared to CXCL4-treated monocytes, indicating more pronounced inflammatory traits for CXCL4L1. In contrast, in CXCL4L1-treated monocytes, the production of CCL22 was lower. Compared to iMDDC generated in the presence of CXCL4L1, CXCL4-treated iMDDC showed an enhanced phagocytic capacity and downregulation of expression of certain surface markers (e.g. CD1a) and specific enzymes (e.g. MMP-9 and MMP-12). CXCL4 and CXCL4L1 did not affect the chemokine receptor expression on iMDDC and cytokine production (CCL2, CCL18, CCL22, CXCL8, IL-10) by CXCL4- or CXCL4L1-differentiated iMDDC was similar. We can conclude that both CXCL4 and CXCL4L1 exert a direct effect on monocytes and iMDDC. However, the resulting phenotypes are different, which suggests a unique role for the two CXCL4 variants in physiology and/or pathology.

Pairwise-interaction extended point-particle model for particle-laden flows

NASA Astrophysics Data System (ADS)

Akiki, G.; Moore, W. C.; Balachandar, S.

2017-12-01

In this work we consider the pairwise interaction extended point-particle (PIEP) model for Euler-Lagrange simulations of particle-laden flows. By accounting for the precise location of neighbors the PIEP model goes beyond local particle volume fraction, and distinguishes the influence of upstream, downstream and laterally located neighbors. The two main ingredients of the PIEP model are (i) the undisturbed flow at any particle is evaluated as a superposition of the macroscale flow and a microscale flow that is approximated as a pairwise superposition of perturbation fields induced by each of the neighboring particles, and (ii) the forces and torque on the particle are then calculated from the undisturbed flow using the Faxén form of the force relation. The computational efficiency of the standard Euler-Lagrange approach is retained, since the microscale perturbation fields induced by a neighbor are pre-computed and stored as PIEP maps. Here we extend the PIEP force model of Akiki et al. [3] with a corresponding torque model to systematically include the effect of perturbation fields induced by the neighbors in evaluating the net torque. Also, we use DNS results from a uniform flow over two stationary spheres to further improve the PIEP force and torque models. We then test the PIEP model in three different sedimentation problems and compare the results against corresponding DNS to assess the accuracy of the PIEP model and improvement over the standard point-particle approach. In the case of two sedimenting spheres in a quiescent ambient the PIEP model is shown to capture the drafting-kissing-tumbling process. In cases of 5 and 80 sedimenting spheres a good agreement is obtained between the PIEP simulation and the DNS. For all three simulations, the DEM-PIEP was able to recreate, to a good extent, the results from the DNS, while requiring only a negligible fraction of the numerical resources required by the fully-resolved DNS.

The utility of polarized heliospheric imaging for space weather monitoring.

PubMed

DeForest, C E; Howard, T A; Webb, D F; Davies, J A

2016-01-01

A polarizing heliospheric imager is a critical next generation tool for space weather monitoring and prediction. Heliospheric imagers can track coronal mass ejections (CMEs) as they cross the solar system, using sunlight scattered by electrons in the CME. This tracking has been demonstrated to improve the forecasting of impact probability and arrival time for Earth-directed CMEs. Polarized imaging allows locating CMEs in three dimensions from a single vantage point. Recent advances in heliospheric imaging have demonstrated that a polarized imager is feasible with current component technology.Developing this technology to a high technology readiness level is critical for space weather relevant imaging from either a near-Earth or deep-space mission. In this primarily technical review, we developpreliminary hardware requirements for a space weather polarizing heliospheric imager system and outline possible ways to flight qualify and ultimately deploy the technology operationally on upcoming specific missions. We consider deployment as an instrument on NOAA's Deep Space Climate Observatory follow-on near the Sun-Earth L1 Lagrange point, as a stand-alone constellation of smallsats in low Earth orbit, or as an instrument located at the Sun-Earth L5 Lagrange point. The critical first step is the demonstration of the technology, in either a science or prototype operational mission context.

Progesterone inhibits the in vitro L3/L4 molting process in Haemonchus contortus.

PubMed

Gutiérrez-Amézquita, R A; Morales-Montor, J; Muñoz-Guzmán, M A; Nava-Castro, K E; Ramírez-Álvarez, H; Cuenca-Verde, C; Moreno-Mendoza, N A; Cuéllar-Ordaz, J A; Alba-Hurtado, F

2017-12-15

We evaluated the direct effects of progesterone on the morphology, maturation and behavior of Haemonchus contortus larvae in vitro. The presence and location of possible progesterone receptors in these larvae were also determined. The addition of 8ng/mL of progesterone to larval cultures over 10days reduced larval enlargement, while the addition of 160ng/mL of the hormone increased the enlargement. Up to 62% and 65% of the H. contortus larvae molted from third-stage larvae (L3) to fourth-stage larvae (L4) when cultured in RPMI-1640 media without hormone for 5 and 10days, respectively. The addition of different progesterone concentrations (1, 8, 16, 80 and 160ng/mL) to the larval cultures significantly inhibited the molting process within the same periods. The addition of 8ng/mL or higher progesterone concentrations to the cultures significantly increased larval motility (p<0.05) compared with unstimulated larvae. Flow cytometry showed the expression of progesterone receptors (P4-R) in 15% of the cells from newly isolated H. contortus larvae. When the larvae were cultured for 5days in the presence of the hormone, the percentage of P 4 -R+ cells remained the same. In contrast, unstimulated larvae showed a significant reduction in the number of P 4 -R+ cells. Using confocal microscopy, a greater concentration of P 4 -Rs was immunolocated in the anterior portion of the alimentary tract of the larvae, suggesting that the cells in this region are targeted by the hormone. The results of the present study show that H. contortus larvae have possible P 4 -Rs and respond to this hormone by inhibiting their molting process, thereby suggesting the participation of progesterone in the larval arrest phenomenon. Copyright © 2017 Elsevier B.V. All rights reserved.

Platelets release CXCL4L1, a nonallelic variant of the chemokine platelet factor-4/CXCL4 and potent inhibitor of angiogenesis.

PubMed

Struyf, Sofie; Burdick, Marie D; Proost, Paul; Van Damme, Jo; Strieter, Robert M

2004-10-29

Platelet factor-4 (PF-4)/CXCL4 was the first chemokine described to inhibit neovascularization. Here, the product of the nonallelic variant gene of CXCL4, PF-4var1/PF-4alt, designated CXCL4L1, was isolated for the first time from thrombin-stimulated human platelets and purified to homogeneity. Although secreted CXCL4 and CXCL4L1 differ in only three amino acids, CXCL4L1 was more potent in inhibiting chemotaxis of human microvascular endothelial cells toward interleukin-8 (IL-8)/CXCL8 or basic fibroblast growth factor (bFGF). In vivo, CXCL4L1 was also more effective than CXCL4 in inhibiting bFGF-induced angiogenesis in rat corneas. Thus, activated platelets release CXCL4L1, a potent regulator of endothelial cell biology, which affects angiogenesis and vascular diseases.

Relativistic semiempirical-core-potential calculations in Ca+,Sr+ , and Ba+ ions on Lagrange meshes

NASA Astrophysics Data System (ADS)

Filippin, Livio; Schiffmann, Sacha; Dohet-Eraly, Jérémy; Baye, Daniel; Godefroid, Michel

2018-01-01

Relativistic atomic structure calculations are carried out in alkaline-earth-metal ions using a semiempirical-core-potential approach. The systems are partitioned into frozen-core electrons and an active valence electron. The core orbitals are defined by a Dirac-Hartree-Fock calculation using the grasp2k package. The valence electron is described by a Dirac-like Hamiltonian involving a core-polarization potential to simulate the core-valence electron correlation. The associated equation is solved with the Lagrange-mesh method, which is an approximate variational approach having the form of a mesh calculation because of the use of a Gauss quadrature to calculate matrix elements. Properties involving the low-lying metastable D 3 /2 ,5 /2 2 states of Ca+, Sr+, and Ba+ are studied, such as polarizabilities, one- and two-photon decay rates, and lifetimes. Good agreement is found with other theory and observation, which is promising for further applications in alkalilike systems.

Conceptual Design of a Synoptic Interplanetary Monitor Platform at L sub 1 (SIMPL).

DTIC Science & Technology

1985-11-01

solar events. -159- . . . .. . 105 II1II" -I .5 year mission at Earth-Sun- libration point plus transfer orbit eDashed line is approximate true dose as...Design .. ...................................... 27 4.1 The L Libration Point .......................... 27 4.2 L Orbit Options...34) to provide power, attitude control, communications, and other support to maintain the instruments in a halo orbit around the L libration point ; 4. a

Soil Moisture Active Passive (SMAP) Mission Level 4 Carbon (L4_C) Product Specification Document

NASA Technical Reports Server (NTRS)

Glassy, Joe; Kimball, John S.; Jones, Lucas; Reichle, Rolf H.; Ardizzone, Joseph V.; Kim, Gi-Kong; Lucchesi, Robert A.; Smith, Edmond B.; Weiss, Barry H.

2015-01-01

This is the Product Specification Document (PSD) for Level 4 Surface and Root Zone Soil Moisture (L4_SM) data for the Science Data System (SDS) of the Soil Moisture Active Passive (SMAP) project. The L4_SM data product provides estimates of land surface conditions based on the assimilation of SMAP observations into a customized version of the NASA Goddard Earth Observing System, Version 5 (GEOS-5) land data assimilation system (LDAS). This document applies to any standard L4_SM data product generated by the SMAP Project.

Spinal case of the month with short perspective: How would you treat this L3-L4 synovial cyst?

PubMed

Epstein, Nancy E

2018-01-01

In this new section, Case of the Month with Short Perspective from Surgical Neurology International, we want to see how various spine surgeons would approach different spinal pathologies. In this first case, an elderly male presented with 3 years of lower back pain and progressive neurogenic claudication with bilateral radiculopathy that had exacerbated over the prior 6 months. An outside physician performed a magnetic resonance (MR) study of the lumbar spine that showed a massive synovial cyst filling the spinal canal (e.g., large bilateral cysts) at the L3-L4 level with grade I spondylolisthesis. The MR and CT studies also both demonstrated moderate L2-L3, and severe L3-L4 stenosis. Despite the massive synovial cyst filling the spinal canal at the L3-L4 level, pain management (anesthesia training) spent 3 months performing three successive epidural steroid injections accompanied by attempts at percutaneous synovial cyst aspiration/rupture. By the time the patient presented to neurosurgery, he had developed severe neurogenic claudication, radiculopathy, myelopathy, and a cauda equina syndrome. Dynamic X-rays revealed a mild grade I degenerative spondylolisthesis at the L3-L4 level without active motion, while both computed tomography (CT) and MR studies confirmed moderate stenosis stenosis/ossification of the yellow ligament at the L2-L3 level, severe stenosis at L3-L4 level with spondylolisthesis, and massive bilateral synovial cysts at the L3-L4 level filling the spinal canal. Following an L2-L4 decompressive laminectomy without fusion (note the absence of motion intraoperatively at the L3-L4 level), the patient's symptoms resolved, and he regained normal function. How would you have managed this patient?

Woofer-tweeter adaptive optics scanning laser ophthalmoscopic imaging based on Lagrange-multiplier damped least-squares algorithm.

PubMed

Zou, Weiyao; Qi, Xiaofeng; Burns, Stephen A

2011-07-01

We implemented a Lagrange-multiplier (LM)-based damped least-squares (DLS) control algorithm in a woofer-tweeter dual deformable-mirror (DM) adaptive optics scanning laser ophthalmoscope (AOSLO). The algorithm uses data from a single Shack-Hartmann wavefront sensor to simultaneously correct large-amplitude low-order aberrations by a woofer DM and small-amplitude higher-order aberrations by a tweeter DM. We measured the in vivo performance of high resolution retinal imaging with the dual DM AOSLO. We compared the simultaneous LM-based DLS dual DM controller with both single DM controller, and a successive dual DM controller. We evaluated performance using both wavefront (RMS) and image quality metrics including brightness and power spectrum. The simultaneous LM-based dual DM AO can consistently provide near diffraction-limited in vivo routine imaging of human retina.

Recombineering reveals a diverse collection of ribosomal proteins L4 and L22 that confer resistance to macrolide antibiotics

PubMed Central

Diner, Elie J.; Hayes, Christopher S.

2009-01-01

Summary Mutations in ribosomal proteins L4 and L22 confer resistance to erythromycin and other macrolide antibiotics in a variety of bacteria. L4 and L22 have elongated loops whose tips converge in the peptide exit tunnel near the macrolide binding site, and resistance mutations typically affect residues within these loops. Here, we use bacteriophage λ Red-mediated recombination, or “recombineering”, to uncover new L4 and L22 alleles that confer macrolide resistance in Escherichia coli. We randomized residues at the tips of the L4 and L22 loops using recombineered oligonucleotide libraries, and selected the mutagenized cells for erythromycin-resistant mutants. These experiments led to the identification of 341 different resistance mutations encoding 278 unique L4 and L22 proteins – the overwhelming majority of which are novel. Many resistance mutations were complex, involving multiple missense mutations, in-frame deletions, and insertions. Transfer of L4 and L22 mutations into wild-type cells by phage P1-mediated transduction demonstrated that each allele was sufficient to confer macrolide resistance. Although L4 and L22 mutants are typically resistant to most macrolides, selections carried out on different antibiotics revealed macrolide-specific resistance mutations. L22 Lys90Trp is one such allele, which confers resistance to erythromycin, but not tylosin or spiramycin. Purified L22 Lys90Trp ribosomes show reduced erythromycin binding, but have the same affinity for tylosin as wild-type ribosomes. Moreover, DMS methylation protection assays demonstrated that L22 Lys90Trp ribosomes bind tylosin more readily than erythromycin in vivo. This work underscores the exceptional functional plasticity of the L4 and L22 proteins, and highlights the utility of Red-mediated recombination in targeted genetic selections. PMID:19150357

Isolation of eukaryotic ribosomal proteins. Purification and characterization of the 60 S ribosomal subunit proteins L4, L5, L7, L9, L11, L12, L13, L21, L22, L23, L26, L27, L30, L33, L35', L37, and L39.

PubMed

Tsurugi, K; Collatz, E; Wool, E G; Lin, A

1976-12-25

The proteins of the large subunit of rat liver ribosomes were separated into seven groups by stepwise elution from carboxymethylcellulose with LiCl at pH 6.5. Seventeen proteins (L4, L5, L7, L9, L11, L12, L13, L21, L22, L23, L26, L27, L30, L33, L35', L37, and L39) were isolated from three of the groups (B60, D60, G60) by ion exchange chromatography on carboxymethylcellulose and by filtration through Sephadex. The amount of protein obtained varied from 0.5 to 15 mg. Eight of the proteins (L9, L11, L13, L21, L22, L35', L37 and L39) had no detectable contamination; the impurities in the others were no greater than 9%. The molecular weight of the proteins was estimated by polyacrylamide gel electrophoresis in sodium dodecyl sulfate; the amino acid composition was determined.

Libration Point Navigation Concepts Supporting Exploration Vision

NASA Technical Reports Server (NTRS)

Carpenter, J. Russell; Folta, David C.; Moreau, Michael C.; Gramling, Cheryl J.

2004-01-01

Farquhar described several libration point navigation concepts that would appear to support NASA s current exploration vision. One concept is a Lunar Relay Satellite operating in the vicinity of Earth-Moon L2, providing Earth-to-lunar far-side and long- range surface-to-surface navigation and communications capability. Reference [ 1] lists several advantages of such a system in comparison to a lunar orbiting relay satellite constellation. Among these are one or two vs. many satellites for coverage, simplified acquisition and tracking due to very low relative motion, much longer contact times, and simpler antenna pointing. An obvious additional advantage of such a system is that uninterrupted links to Earth avoid performing critical maneuvers "in the blind." Another concept described is the use of Earth-Moon L1 for lunar orbit rendezvous, rather than low lunar orbit as was done for Apollo. This rendezvous technique would avoid large plane change and high fuel cost associated with high latitude landing sites and long stay times. Earth-Moon L1 also offers unconstrained launch windows from the lunar surface. Farquhar claims this technique requires only slightly higher fuel cost than low lunar orbit rendezvous for short-stay equatorial landings. Farquhar also describes an Interplanetary Transportation System that would use libration points as terminals for an interplanetary shuttle. This approach would offer increased operational flexibility in terms of launch windows, rendezvous, aborts, etc. in comparison to elliptical orbit transfers. More recently, other works including Folta[3] and Howell[4] have shown that patching together unstable trajectories departing Earth-Moon libration points with stable trajectories approaching planetary libration points may also offer lower overall fuel costs than elliptical orbit transfers. Another concept Farquhar described was a Deep Space Relay at Earth-Moon IA and/or L5 that would serve as a high data rate optical navigation and

Incomplete Recovery of CD4 count, CD4 Percentage, and CD4/CD8 ratio in HIV-Infected Patients on Long-Term Antiretroviral Therapy with Suppressed Viremia.

PubMed

Mutoh, Yoshikazu; Nishijima, Takeshi; Inaba, Yosuke; Tanaka, Noriko; Kikuchi, Yoshimi; Gatanaga, Hiroyuki; Oka, Shinichi

2018-03-02

The extent and duration of long-term recovery of CD4 count, CD4%, and CD4/CD8 ratio after initiation of combination antiretroviral therapy (cART) in patients with suppressed viral load are largely unknown. HIV-1 infected patients who started cART between January 2004 and January 2012 and showed persistent viral suppression (<200 copies/mL) for at least 4 years were followed up at AIDS Clinical Center, Tokyo. Change point analysis was used to determine the time point where CD4 count recovery shows a plateau, and linear mixed model was applied to estimate CD4 count at the change point. Data of 752 patients were analyzed [93% males, median age 38, median baseline CD4 count 172/µL (IQR, 62-253), CD4% 13.8% (IQR, 7.7-18.5), and CD4/8 ratio 0.23 (IQR, 0.12-0.35)]. The median follow-up period was 81.2 months and 91 (12.1%) patients were followed for >10 years. Change point analysis showed that CD4 count, CD4%, and CD4/CD8 ratio, continued to increase until 78.6, 62.2, and 64.3 months, respectively, with adjusted mean of 590 /µL (95%CI 572-608), 29.5% (29-30.1), and 0.89 (0.86-0.93), respectively, at the change point. Although 73.8% of the study patients achieved CD4 count ≥500 /μL, 48.2% of the patients with baseline CD4 count <100 /μL did not achieve CD4 count ≥500 /μL. Neither CD4% nor CD4/CD8 ratio normalized in a majority of patients. The results showed lack of normalization of CD4 count, CD4%, and CD4/CD8 ratio to the levels seen in healthy individuals even after long-term successful cART in patients with suppressed viral load.

Blow-up solutions for L 2 supercritical gKdV equations with exactly k blow-up points

NASA Astrophysics Data System (ADS)

Lan, Yang

2017-08-01

In this paper we consider the slightly L 2-supercritical gKdV equations \\partialt u+(uxx+u\\vert u\\vert p-1)_x=0 , with the nonlinearity 5 and 0<\\varepsilon\\ll 1 . In the previous work of the author, we know that there exists a stable self-similar blow-up dynamics for slightly L 2-supercritical gKdV equations. Such solutions can be viewed as solutions with a single blow-up point. In this paper we will prove the existence of solutions with multiple blow-up points, and give a description of the formation of the singularity near the blow-up time.

Adapting the Euler-Lagrange equation to study one-dimensional motions under the action of a constant force

NASA Astrophysics Data System (ADS)

Dias, Clenilda F.; Araújo, Maria A. S.; Carvalho-Santos, Vagson L.

2018-01-01

The Euler-Lagrange equations (ELE) are very important in the theoretical description of several physical systems. In this work we have used a simplified form of ELE to study one-dimensional motions under the action of a constant force. From the use of the definition of partial derivative, we have proposed two operators, here called mean delta operators, which may be used to solve the ELE in a simplest way. We have applied this simplification to solve three simple mechanical problems in which the particle is under the action of the gravitational field: a free fall body, the Atwood’s machine and the inclined plan. The proposed simplification can be used to introduce the lagrangian formalism in teaching classical mechanics in introductory physics courses.

Trajectory Design Strategies for the NGST L2 Libration Point Mission

NASA Technical Reports Server (NTRS)

Folta, David; Cooley, Steven; Howell, Kathleen; Bauer, Frank H.

2001-01-01

The Origins' Next Generation Space Telescope (NGST) trajectory design is addressed in light of improved methods for attaining constrained orbit parameters and their control at the exterior collinear libration point, L2. The use of a dynamical systems approach, state-space equations for initial libration orbit control, and optimization to achieve constrained orbit parameters are emphasized. The NGST trajectory design encompasses a direct transfer and orbit maintenance under a constant acceleration. A dynamical systems approach can be used to provide a biased orbit and stationkeeping maintenance method that incorporates the constraint of a single axis correction scheme.

Structural Basis for Recognition of L-lysine, L-ornithine, and L-2,4-diamino Butyric Acid by Lysine Cyclodeaminase.

PubMed

Min, Kyungjin; Yoon, Hye-Jin; Matsuura, Atsushi; Kim, Yong Hwan; Lee, Hyung Ho

2018-04-30

L-pipecolic acid is a non-protein amino acid commonly found in plants, animals, and microorganisms. It is a well-known precursor to numerous microbial secondary metabolites and pharmaceuticals, including anticancer agents, immunosuppressants, and several antibiotics. Lysine cyclodeaminase (LCD) catalyzes β-deamination of L-lysine into L-pipecolic acid using β-nicotinamide adenine dinucleotide as a cofactor. Expression of a human homolog of LCD, μ-crystallin, is elevated in prostate cancer patients. To understand the structural features and catalytic mechanisms of LCD, we determined the crystal structures of Streptomyces pristinaespiralis LCD (SpLCD) in (i) a binary complex with NAD + , (ii) a ternary complex with NAD + and L-pipecolic acid, (iii) a ternary complex with NAD + and L-proline, and (iv) a ternary complex with NAD + and L-2,4-diamino butyric acid. The overall structure of SpLCD was similar to that of ornithine cyclodeaminase from Pseudomonas putida . In addition, SpLCD recognized L-lysine, L-ornithine, and L-2,4-diamino butyric acid despite differences in the active site, including differences in hydrogen bonding by Asp236, which corresponds with Asp228 from Pseudomonas putida ornithine cyclodeaminase. The substrate binding pocket of SpLCD allowed substrates smaller than lysine to bind, thus enabling binding to ornithine and L-2,4-diamino butyric acid. Our structural and biochemical data facilitate a detailed understanding of substrate and product recognition, thus providing evidence for a reaction mechanism for SpLCD. The proposed mechanism is unusual in that NAD + is initially converted into NADH and then reverted back into NAD + at a late stage of the reaction.

Determination of the Minimum Delta V Transfer Trajectory from a Low Earth Orbit to a Stable Orbit Around the Lagrangian Point L4 in a Restricted Four-Body System.

DTIC Science & Technology

1979-12-01

the Moon revolves around the Earth. T. A . Heppenheimer (Ref 5) and his colleagues B. O’Leary and D. Kaplan (Ref 7) have also examined the pro- blem of...colony location and transfer trajectories. Heppenheimer found a 2/1 resonant orbit around the Earth 2S that could be reached from L2 by a Hohmann...would not allow a catcher near either of these points. In their work together, O’Leary, Kaplan and Heppenheimer found a transfer trajectory from L2

Detection of high PD-L1 expression in oral cancers by a novel monoclonal antibody L1Mab-4.

PubMed

Yamada, Shinji; Itai, Shunsuke; Kaneko, Mika K; Kato, Yukinari

2018-03-01

Programmed cell death-ligand 1 (PD-L1), which is a ligand of programmed cell death-1 (PD-1), is a type I transmembrane glycoprotein that is expressed on antigen-presenting cells and several tumor cells, including melanoma and lung cancer cells. There is a strong correlation between human PD-L1 (hPD-L1) expression on tumor cells and negative prognosis in cancer patients. In this study, we produced a novel anti-hPD-L1 monoclonal antibody (mAb), L 1 Mab-4 (IgG 2b , kappa), using cell-based immunization and screening (CBIS) method and investigated hPD-L1 expression in oral cancers. L 1 Mab-4 reacted with oral cancer cell lines (Ca9-22, HO-1-u-1, SAS, HSC-2, HSC-3, and HSC-4) in flow cytometry and stained oral cancers in a membrane-staining pattern. L 1 Mab-4 stained 106/150 (70.7%) of oral squamous cell carcinomas, indicating the very high sensitivity of L 1 Mab-4. These results indicate that L 1 Mab-4 could be useful for investigating the function of hPD-L1 in oral cancers.

Perturbed Equations of Motion for Formation Flight Near the Sun-Earth L2 Point

NASA Technical Reports Server (NTRS)

Segerman, Alan M.; Zedd, Michael F.

2005-01-01

This Memorandum Report consists of a compilation of three individual reports, of increasing complexity, describing investigations of formation flight of spacecraft in the vicinity of the L2 Sun-Earth 1ibration point. The individual reports form the following parts of this compilation: - Introduction to the relative motion of spacecraft about the Sun-Earth L2 Point - Linear and quadratic modelling and solution of the relative motion - Modelling the Perturbations - Elliptical Earth Orbit, Lunar Gravity, Solar Radiation Pressure, Thrusters. The three parts are self-contained, with somewhat, varying notation and terminology. After fair1y significant literature searches: this new work (of Parts 2 and 3) is deemed to be unique because it describes the primary perturbations to the description of relative motion between nearby spacecraft. The effect of the elliptical motion of the Earth about the Sun was verified to be the dominant perturbation to the circular restricted three body problem. Contributions due to lunar gravity and solar radiation pressure are seen to have much smaller effect.

A dispersive treatment of K l4 decays

DOE PAGES

Colangelo, Gilberto; Passemar, Emilie; Stoffer, Peter

2015-04-28

K l4 decays offer several reasons of interest: they allow an accurate measurement of ππ-scattering lengths; they provide the best source for the determination of some low-energy constants of xPT; one form factor is directly related to the chiral anomaly, which can be measured here. We present a dispersive treatment of K l4 decays that provides a resummation of ππ- and K π-rescattering effects. In addition, the free parameters of the dispersion relation are fitted to the data of the high-statistics experiments E865 and NA48/2. The matching to xPT at NLO and NNLO enables us to determine the LECs Lmore » r 1, L r 2 and L r 3. With recently published data from NA48/2, the LEC L r 9 can be determined as well. In contrast to a pure chiral treatment, the dispersion relation describes the observed curvature of one of the form factors, which we understand as a rescattering effect beyond NNLO.« less

Space Instrument Optimization by Implementing of Generic Three Bodies Circular Restricted Problem

NASA Astrophysics Data System (ADS)

Nejat, Cyrus

2011-01-01

In this study, the main discussion emphasizes on the spacecraft operation with a concentration on stationary points in space. To achieve these objectives, the circular restricted problem was solved for selected approaches. The equations of motion of three body restricted problem was demonstrated to apply in cases other than Lagrange's (1736-1813 A.D.) achievements, by means of the purposed CN (Cyrus Nejat) theorem along with appropriate comments. In addition to five Lagrange, two other points, CN1 and CN2 were found to be in unstable equilibrium points in a very large distance respect to Lagrange points, but stable at infinity. A very interesting simulation of Milky Way Galaxy and Andromeda Galaxy were created to find the Lagrange points, CN points (Cyrus Nejat Points), and CN lines (Cyrus Nejat Lines). The equations of motion were rearranged such a way that the transfer trajectory would be conical, by means of decoupling concept. The main objective was to make a halo orbit transfer about CN lines. The author purposes therefore that all of the corresponding sizing design that they must be developed by optimization techniques would be considered in future approaches. The optimization techniques are sufficient procedures to search for the most ideal response of a system.

An Euler-Lagrange method considering bubble radial dynamics for modeling sonochemical reactors.

PubMed

Jamshidi, Rashid; Brenner, Gunther

2014-01-01

Unsteady numerical computations are performed to investigate the flow field, wave propagation and the structure of bubbles in sonochemical reactors. The turbulent flow field is simulated using a two-equation Reynolds-Averaged Navier-Stokes (RANS) model. The distribution of the acoustic pressure is solved based on the Helmholtz equation using a finite volume method (FVM). The radial dynamics of a single bubble are considered by applying the Keller-Miksis equation to consider the compressibility of the liquid to the first order of acoustical Mach number. To investigate the structure of bubbles, a one-way coupling Euler-Lagrange approach is used to simulate the bulk medium and the bubbles as the dispersed phase. Drag, gravity, buoyancy, added mass, volume change and first Bjerknes forces are considered and their orders of magnitude are compared. To verify the implemented numerical algorithms, results for one- and two-dimensional simplified test cases are compared with analytical solutions. The results show good agreement with experimental results for the relationship between the acoustic pressure amplitude and the volume fraction of the bubbles. The two-dimensional axi-symmetric results are in good agreement with experimentally observed structure of bubbles close to sonotrode. Copyright © 2013 Elsevier B.V. All rights reserved.

Soil Moisture Active Passive Mission L4_SM Data Product Assessment (Version 2 Validated Release)

NASA Technical Reports Server (NTRS)

Reichle, Rolf Helmut; De Lannoy, Gabrielle J. M.; Liu, Qing; Ardizzone, Joseph V.; Chen, Fan; Colliander, Andreas; Conaty, Austin; Crow, Wade; Jackson, Thomas; Kimball, John;

Identification of the polypeptides encoded in the unassigned reading frames 2, 4, 4L, and 5 of human mitochondrial DNA.

PubMed Central

Mariottini, P; Chomyn, A; Riley, M; Cottrell, B; Doolittle, R F; Attardi, G

1986-01-01

In previous work, antibodies prepared against chemically synthesized peptides predicted from the DNA sequence were used to identify the polypeptides encoded in three of the eight unassigned reading frames (URFs) of human mitochondrial DNA (mtDNA). In the present study, this approach has been extended to other human mtDNA URFs. In particular, antibodies directed against the NH2-terminal octapeptide of the putative URF2 product specifically precipitated component 11 of the HeLa cell mitochondrial translation products, the reaction being inhibited by the specific peptide. Similarly, antibodies directed against the COOH-terminal nonapeptide of the putative URF4 product reacted specifically with components 4 and 5, and antibodies against a COOH-terminal heptapeptide of the presumptive URF4L product reacted specifically with component 26. Antibodies against the NH2-terminal heptapeptide of the putative product of URF5 reacted with component 1, but only to a marginal extent; however, the results of a trypsin fingerprinting analysis of component 1 point strongly to this component as being the authentic product of URF5. The polypeptide assignments to the mtDNA URFs analyzed here are supported by the relative electrophoretic mobilities of proteins 11, 4-5, 26, and 1, which are those expected for the molecular weights predicted from the DNA sequence for the products of URF2, URF4, URF4L, and URF5, respectively. With the present assignment, seven of the eight human mtDNA URFs have been shown to be expressed in HeLa cells. Images PMID:3456601

Distributed adaptive asymptotically consensus tracking control of uncertain Euler-Lagrange systems under directed graph condition.

PubMed

Wang, Wei; Wen, Changyun; Huang, Jiangshuai; Fan, Huijin

2017-11-01

In this paper, a backstepping based distributed adaptive control scheme is proposed for multiple uncertain Euler-Lagrange systems under directed graph condition. The common desired trajectory is allowed totally unknown by part of the subsystems and the linearly parameterized trajectory model assumed in currently available results is no longer needed. To compensate the effects due to unknown trajectory information, a smooth function of consensus errors and certain positive integrable functions are introduced in designing virtual control inputs. Besides, to overcome the difficulty of completely counteracting the coupling terms of distributed consensus errors and parameter estimation errors in the presence of asymmetric Laplacian matrix, extra information transmission of local parameter estimates are introduced among linked subsystem and adaptive gain technique is adopted to generate distributed torque inputs. It is shown that with the proposed distributed adaptive control scheme, global uniform boundedness of all the closed-loop signals and asymptotically output consensus tracking can be achieved. Copyright © 2017 ISA. Published by Elsevier Ltd. All rights reserved.

Generalized concurrence measure for faithful quantification of multiparticle pure state entanglement using Lagrange's identity and wedge product

NASA Astrophysics Data System (ADS)

Bhaskara, Vineeth S.; Panigrahi, Prasanta K.

2017-05-01

Concurrence, introduced by Hill and Wootters (Phys Rev Lett 78:5022, 1997), provides an important measure of entanglement for a general pair of qubits that is faithful: strictly positive for entangled states and vanishing for all separable states. Such a measure captures the entire content of entanglement, providing necessary and sufficient conditions for separability. We present an extension of concurrence to multiparticle pure states in arbitrary dimensions by a new framework using the Lagrange's identity and wedge product representation of separability conditions, which coincides with the "I-concurrence" of Rungta et al. (Phys Rev A 64:042315, 2001) who proposed by extending Wootters's spin-flip operator to a so-called universal inverter superoperator. Our framework exposes an inherent geometry of entanglement and may be useful for the further extensions to mixed and continuous variable states.

26 CFR 36.3121(l)(4)-1 - Termination of agreement by Commissioner.

Code of Federal Regulations, 2010 CFR

2010-04-01

....3121(l)(4)-1 Section 36.3121(l)(4)-1 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE... OF FOREIGN SUBSIDIARIES § 36.3121(l)(4)-1 Termination of agreement by Commissioner. (a) Notice of....3121(l)(1)-1 is in effect may be terminated by the Commissioner, with the prior concurrence of the...

On some 3-point functions in the W 4 CFT and related braiding matrix

NASA Astrophysics Data System (ADS)

Furlan, P.; Petkova, V. B.

2015-12-01

We construct a class of 3-point constants in the sl(4) Toda conformal theory W 4, extending the examples in Fateev and Litvinov [1]. Their knowledge allows to determine the braiding/fusing matrix transforming 4-point conformal blocks of one fundamental, labelled by the 6-dimensional sl(4) representation, and three partially degenerate vertex operators. It is a 3 × 3 submatrix of the generic 6 × 6 fusing matrix consistent with the fusion rules for the particular class of representations. We check a braiding relation which has wider applications to conformal models with sl(4) symmetry. The 3-point constants in dual regions of central charge are compared in preparation for a BPS like relation in the widehat{sl}(4) WZW model.

Controlling the transmission of stereochemical information through space in terphenyl-edged Fe4L6 cages.

PubMed

Meng, Wenjing; Clegg, Jack K; Thoburn, John D; Nitschke, Jonathan R

2011-08-31

A series of terphenyl-edged Fe(4)L(6) cages were synthesized from substituted 4,4''-diamino-p-terphenyls, 2-formylpyridine, and iron(II). For the parent diaminoterphenyl, all three possible diastereomers, with T, S(4), and C(3) point symmetries, were formed in nearly equal amounts, as determined by (1)H and (13)C NMR. When 2,2″-dimethylterphenylenediamine was used, the T-symmetry diastereomer was observed to predominate. The use of 2',3',5',6'-tetramethylterphenylenediamine generated predominantly the S(4) cage diastereomer, whereas 2',5'-dimethylterphenylenediamine produced the C(3)-symmetric cage to a greater degree than the other two diastereomers. The factors contributing to the transfer of chiral information between metal vertices were analyzed, and the general principles underlying the delicately balanced thermodynamics were determined.

A Node Localization Algorithm Based on Multi-Granularity Regional Division and the Lagrange Multiplier Method in Wireless Sensor Networks.

PubMed

Shang, Fengjun; Jiang, Yi; Xiong, Anping; Su, Wen; He, Li

2016-11-18

With the integrated development of the Internet, wireless sensor technology, cloud computing, and mobile Internet, there has been a lot of attention given to research about and applications of the Internet of Things. A Wireless Sensor Network (WSN) is one of the important information technologies in the Internet of Things; it integrates multi-technology to detect and gather information in a network environment by mutual cooperation, using a variety of methods to process and analyze data, implement awareness, and perform tests. This paper mainly researches the localization algorithm of sensor nodes in a wireless sensor network. Firstly, a multi-granularity region partition is proposed to divide the location region. In the range-based method, the RSSI (Received Signal Strength indicator, RSSI) is used to estimate distance. The optimal RSSI value is computed by the Gaussian fitting method. Furthermore, a Voronoi diagram is characterized by the use of dividing region. Rach anchor node is regarded as the center of each region; the whole position region is divided into several regions and the sub-region of neighboring nodes is combined into triangles while the unknown node is locked in the ultimate area. Secondly, the multi-granularity regional division and Lagrange multiplier method are used to calculate the final coordinates. Because nodes are influenced by many factors in the practical application, two kinds of positioning methods are designed. When the unknown node is inside positioning unit, we use the method of vector similarity. Moreover, we use the centroid algorithm to calculate the ultimate coordinates of unknown node. When the unknown node is outside positioning unit, we establish a Lagrange equation containing the constraint condition to calculate the first coordinates. Furthermore, we use the Taylor expansion formula to correct the coordinates of the unknown node. In addition, this localization method has been validated by establishing the real environment.

Evaluation of a transient, simultaneous, arbitrary Lagrange-Euler based multi-physics method for simulating the mitral heart valve.

PubMed

Espino, Daniel M; Shepherd, Duncan E T; Hukins, David W L

2014-01-01

A transient multi-physics model of the mitral heart valve has been developed, which allows simultaneous calculation of fluid flow and structural deformation. A recently developed contact method has been applied to enable simulation of systole (the stage when blood pressure is elevated within the heart to pump blood to the body). The geometry was simplified to represent the mitral valve within the heart walls in two dimensions. Only the mitral valve undergoes deformation. A moving arbitrary Lagrange-Euler mesh is used to allow true fluid-structure interaction (FSI). The FSI model requires blood flow to induce valve closure by inducing strains in the region of 10-20%. Model predictions were found to be consistent with existing literature and will undergo further development.

Site-specific incorporation of 4-iodo-L-phenylalanine through opal suppression.

PubMed

Kodama, Koichiro; Nakayama, Hiroshi; Sakamoto, Kensaku; Fukuzawa, Seketsu; Kigawa, Takanori; Yabuki, Takashi; Kitabatake, Makoto; Takio, Koji; Yokoyama, Shigeyuki

2010-08-01

A variety of unique codons have been employed to expand the genetic code. The use of the opal (UGA) codon is promising, but insufficient information is available about the UGA suppression approach, which facilitates the incorporation of non-natural amino acids through suppression of the UGA codon. In this study, the UGA codon was used to incorporate 4-iodo-l-phenylalanine into position 32 of the Ras protein in an Escherichia coli cell-free translation system. The undesired incorporation of tryptophan in response to the UGA codon was completely repressed by the addition of indolmycin. The minor amount (3%) of contaminating 4-bromo-l-phenylalanine in the building block 4-iodo-l-phenylalanine led to the significant incorporation of 4-bromo-l-phenylalanine (21%), and this problem was solved by using a purified 4-iodo-l-phenylalanine sample. Optimization of the incubation time was also important, since the undesired incorporation of free phenylalanine increased during the cell-free translation reaction. The 4-iodo-l-phenylalanine residue can be used for the chemoselective modification of proteins. This method will contribute to advancements in protein engineering studies with non-natural amino acid substitutions.

Influence of a weak gravitational wave on a bound system of two point-masses. [of binary stars

NASA Technical Reports Server (NTRS)

Turner, M. S.

1979-01-01

The problem of a weak gravitational wave impinging upon a nonrelativistic bound system of two point masses is considered. The geodesic equation for each mass is expanded in terms of two small parameters, v/c and dimensionless wave amplitude, in a manner similar to the post-Newtonian expansion; the geodesic equations are resolved into orbital and center-of-mass equations of motion. The effect of the wave on the orbit is determined by using Lagrange's planetary equations to calculate the time evolution of the orbital elements. The gauge properties of the solutions and, in particular, the gauge invariance of the secular effects are discussed.

First observations of volcanic eruption clouds from L1 by DSCOVR/EPIC

NASA Astrophysics Data System (ADS)

Carn, S. A.; Krotkov, N. A.; Taylor, S.; Fisher, B. L.; Li, C.; Hughes, E. J.; Bhartia, P. K.; Prata, F.

2016-12-01

Volcanic emissions of sulfur dioxide (SO2) and ash have been measured by ultraviolet (UV) sensors on US and European polar-orbiting satellites since the late 1970s. Although successful, the main limitation of these UV observations from low-Earth orbit has been poor temporal resolution. Timeliness can be crucial when detecting hazardous volcanic eruption clouds that threaten aviation, and most operational geostationary satellites cannot detect SO2, a key tracer of volcanic plumes. In 2015, the launch of the Earth Polychromatic Imaging Camera (EPIC) aboard the Deep Space Climate Observatory (DSCOVR) provided the first opportunity to observe volcanic clouds from the L1 Lagrange point. EPIC is a 10-band spectroradiometer spanning UV to near-IR wavelengths with two UV channels sensitive to SO2, and a ground resolution of 25 km. The unique L1 vantage point provides continuous observations of the sunlit Earth disk, potentially offering multiple daily observations of volcanic SO2 and ash clouds in the EPIC field of view. When coupled with complementary retrievals from polar-orbiting UV and infrared (IR) sensors such as the Ozone Monitoring Instrument (OMI), the Ozone Mapping and Profiler Suite (OMPS), and the Atmospheric Infrared Sounder (AIRS), the increased observation frequency afforded by DSCOVR/EPIC will permit more timely volcanic eruption detection, improved trajectory modeling, and novel analyses of the temporal evolution of volcanic clouds. We demonstrate the sensitivity of EPIC UV radiances to volcanic clouds using examples from the first year of EPIC observations including the December 2015 paroxysmal eruption of Etna volcano (Italy). When combined with OMI and OMPS measurements, the EPIC SO2 data permit hourly tracking of the Etna eruption cloud as it drifts away from the volcano. We also describe ongoing efforts to adapt existing UV backscatter (BUV) algorithms to produce operational EPIC SO2 and Ash Index (AI) products.

Four-point functions and the permutation group S4

NASA Astrophysics Data System (ADS)

Eichmann, Gernot; Fischer, Christian S.; Heupel, Walter

2015-09-01

Four-point functions are at the heart of many interesting physical processes. A prime example is the light-by-light scattering amplitude, which plays an important role in the calculation of hadronic contributions to the anomalous magnetic moment of the muon. In the calculation of such quantities one faces the challenge of finding a suitable and well-behaved basis of tensor structures in coordinate and/or momentum space. Provided all (or many) of the external legs represent similar particle content, a powerful tool to construct and organize such bases is the permutation group S4. We introduce an efficient notation for dealing with the irreducible multiplets of S4, and we highlight the merits of this treatment by exemplifying four-point functions with gauge-boson legs such as the four-gluon vertex and the light-by-light scattering amplitude. The multiplet analysis is also useful for isolating the important kinematic regions and the dynamical singularity content of such amplitudes. Our analysis serves as a basis for future efficient calculations of these and similar objects.

Foliage penetration by using 4-D point cloud data

NASA Astrophysics Data System (ADS)

Méndez Rodríguez, Javier; Sánchez-Reyes, Pedro J.; Cruz-Rivera, Sol M.

2012-06-01

Real-time awareness and rapid target detection are critical for the success of military missions. New technologies capable of detecting targets concealed in forest areas are needed in order to track and identify possible threats. Currently, LAser Detection And Ranging (LADAR) systems are capable of detecting obscured targets; however, tracking capabilities are severely limited. Now, a new LADAR-derived technology is under development to generate 4-D datasets (3-D video in a point cloud format). As such, there is a new need for algorithms that are able to process data in real time. We propose an algorithm capable of removing vegetation and other objects that may obfuscate concealed targets in a real 3-D environment. The algorithm is based on wavelets and can be used as a pre-processing step in a target recognition algorithm. Applications of the algorithm in a real-time 3-D system could help make pilots aware of high risk hidden targets such as tanks and weapons, among others. We will be using a 4-D simulated point cloud data to demonstrate the capabilities of our algorithm.

Stimulation of hepatic glutathione formation by administration of L-2-oxothiazolidine-4-carboxylate, a 5-oxo-L-prolinase substrate.

PubMed Central

Williamson, J M; Meister, A

1981-01-01

5-Oxo-L-prolinase, the enzyme that catalyzes the conversion of 5-oxo-L-proline to L-glutamate coupled to the cleavage of ATP to ADP and Pi, also acts on L-2-oxothiazolidine-4-carboxylate (an analog of 5-oxoproline in which the 4-methylene moiety is replaced by sulfur) and ATP to yield cysteine and ADP. The enzyme, which exhibits an affinity for the analog similar to that for the natural substrate, is inhibited by the analog in vitro and in vivo. L-2-oxothiazolidine-4-carboxylate thus serves as a potent inhibitor of the gamma-glutamyl cycle at the step of 5-oxoprolinase. Administration of L-2-oxothiazolidine-4-carboxylate to mice that had been depleted of hepatic glutathione led to restoration of normal hepatic glutathione levels. Since L-2-oxothiazolidine-4-carboxylate is an excellent substrate of the enzyme, it may serve as an intracellular delivery system for cysteine and thus has potential as a therapeutic agent for conditions in which there is depletion of hepatic glutathione. PMID:6940159

Intracellular L-arginine concentration does not determine NO production in endothelial cells: Implications on the 'L-arginine paradox'

DOE Office of Scientific and Technical Information (OSTI.GOV)

Shin, Soyoung; Mohan, Srinidi; Fung, Ho-Leung, E-mail: hlfung@buffalo.edu

2011-11-04

Highlights: Black-Right-Pointing-Pointer Our findings provide a possible solution to the 'L-arginine paradox'. Black-Right-Pointing-Pointer Extracellular L-arginine concentration is the major determinant of NO production. Black-Right-Pointing-Pointer Cellular L-arginine action is limited by cellular ARG transport, not the K{sub m} of NOS. Black-Right-Pointing-Pointer We explain how L-arginine supplementation can work to increase endothelial function. -- Abstract: We examined the relative contributory roles of extracellular vs. intracellular L-arginine (ARG) toward cellular activation of endothelial nitric oxide synthase (eNOS) in human endothelial cells. EA.hy926 human endothelial cells were incubated with different concentrations of {sup 15}N{sub 4}-ARG, ARG, or L-arginine ethyl ester (ARG-EE) for 2 h.more » To modulate ARG transport, siRNA for ARG transporter (CAT-1) vs. sham siRNA were transfected into cells. ARG transport activity was assessed by cellular fluxes of ARG, {sup 15}N{sub 4}-ARG, dimethylarginines, and L-citrulline by an LC-MS/MS assay. eNOS activity was determined by nitrite/nitrate accumulation, either via a fluorometric assay or by{sup 15}N-nitrite or estimated {sup 15}N{sub 3}-citrulline concentrations when {sup 15}N{sub 4}-ARG was used to challenge the cells. We found that ARG-EE incubation increased cellular ARG concentration but no increase in nitrite/nitrate was observed, while ARG incubation increased both cellular ARG concentration and nitrite accumulation. Cellular nitrite/nitrate production did not correlate with cellular total ARG concentration. Reduced {sup 15}N{sub 4}-ARG cellular uptake in CAT-1 siRNA transfected cells vs. control was accompanied by reduced eNOS activity, as determined by {sup 15}N-nitrite, total nitrite and {sup 15}N{sub 3}-citrulline formation. Our data suggest that extracellular ARG, not intracellular ARG, is the major determinant of NO production in endothelial cells. It is likely that once transported inside

Angiostatic and chemotactic activities of the CXC chemokine CXCL4L1 (platelet factor-4 variant) are mediated by CXCR3

PubMed Central

Struyf, Sofie; Salogni, Laura; Burdick, Marie D.; Vandercappellen, Jo; Gouwy, Mieke; Noppen, Sam; Proost, Paul; Opdenakker, Ghislain; Parmentier, Marc; Gerard, Craig; Sozzani, Silvano; Strieter, Robert M.

2011-01-01

We investigated possible cellular receptors for the human CXC chemokine platelet factor-4 variant/CXCL4L1, a potent inhibitor of angiogenesis. We found that CXCL4L1 has lower affinity for heparin and chondroitin sulfate-E than platelet factor-4 (CXCL4) and showed that CXCL10 and CXCL4L1 could displace each other on microvascular endothelial cells. Labeled CXCL4L1 also bound to CXCR3A- and CXCR3B-transfectants and was displaced by CXCL4L1, CXCL4, and CXCL10. The CXCL4L1 anti-angiogenic activity was blocked by anti-CXCR3 antibodies (Abs) in the Matrigel and cornea micropocket assays. CXCL4L1 application in CXCR3−/− or in wild-type mice treated with neutralizing anti-CXCR3 Abs, resulted in reduced inhibitory activity of CXCL4L1 on tumor growth and vascularization of Lewis lung carcinoma. Furthermore, CXCL4L1 and CXCL4 chemoattracted activated T cells, human natural killer cells, and human immature dendritic cells (DCs). Migration of DCs toward CXCL4 and CXCL4L1 was desensitized by preincubation with CXCL10 and CXCL11, inhibited by pertussis toxin, and neutralized by anti-CXCR3 Abs. Chemotaxis of T cells, natural killer cells, and DCs is likely to contribute to the antitumoral action. However, the in vivo data indicate that the angiostatic property of CXCL4L1 is equally important in retarding tumor growth. Thus, both CXCR3A and CXCR3B are implicated in the chemotactic and vascular effects of CXCL4L1. PMID:20980681

Angiostatic and chemotactic activities of the CXC chemokine CXCL4L1 (platelet factor-4 variant) are mediated by CXCR3.

PubMed

Struyf, Sofie; Salogni, Laura; Burdick, Marie D; Vandercappellen, Jo; Gouwy, Mieke; Noppen, Sam; Proost, Paul; Opdenakker, Ghislain; Parmentier, Marc; Gerard, Craig; Sozzani, Silvano; Strieter, Robert M; Van Damme, Jo

2011-01-13

We investigated possible cellular receptors for the human CXC chemokine platelet factor-4 variant/CXCL4L1, a potent inhibitor of angiogenesis. We found that CXCL4L1 has lower affinity for heparin and chondroitin sulfate-E than platelet factor-4 (CXCL4) and showed that CXCL10 and CXCL4L1 could displace each other on microvascular endothelial cells. Labeled CXCL4L1 also bound to CXCR3A- and CXCR3B-transfectants and was displaced by CXCL4L1, CXCL4, and CXCL10. The CXCL4L1 anti-angiogenic activity was blocked by anti-CXCR3 antibodies (Abs) in the Matrigel and cornea micropocket assays. CXCL4L1 application in CXCR3(-/-) or in wild-type mice treated with neutralizing anti-CXCR3 Abs, resulted in reduced inhibitory activity of CXCL4L1 on tumor growth and vascularization of Lewis lung carcinoma. Furthermore, CXCL4L1 and CXCL4 chemoattracted activated T cells, human natural killer cells, and human immature dendritic cells (DCs). Migration of DCs toward CXCL4 and CXCL4L1 was desensitized by preincubation with CXCL10 and CXCL11, inhibited by pertussis toxin, and neutralized by anti-CXCR3 Abs. Chemotaxis of T cells, natural killer cells, and DCs is likely to contribute to the antitumoral action. However, the in vivo data indicate that the angiostatic property of CXCL4L1 is equally important in retarding tumor growth. Thus, both CXCR3A and CXCR3B are implicated in the chemotactic and vascular effects of CXCL4L1.

Preliminary Planar Formation: Flight Dynamics Near Sun-Earth L2 Point

NASA Technical Reports Server (NTRS)

Segerman, Alan M.; Zedd, Michael F.

2003-01-01

NASA's Goddard Space Flight Center is planning a series of missions in the vicinity of the Sun-Earth L2 libration point. Some of these projects will involve a distributed space system of telescope spacecraft acting together as a single telescope for high-resolution. The individual telescopes will be configured in a plane, surrounding a hub, where the telescope plane can be aimed toward various astronomical targets of interest. In preparation for these missions, it is necessary to develop an improved understanding of the dynamical behavior of objects in a planar configuration near L2. The classical circular restricted three body problem is taken as the basis for the analysis. At first order, the motion of such a telescope relative to the hub is described by a system of linear second order differential equations. These equations are identical to the circular restricted problem's linear equations describing the hub motion about L2. Therefore, the fundamental frequencies, both parallel to and normal to the ecliptic plane, are the same for the relative telescope motion as for the hub motion. To maintain the telescope plane for the duration necessary for the planned observations, a halo-type orbit of the telescopes about the hub is investigated. By using a halo orbit, the individual telescopes remain in approximately the same plane over the observation duration. For such an orbit, the fundamental periods parallel to and normal to the ecliptic plane are forced to be the same by careful selection of the initial conditions in order to adjust the higher order forces. The relative amplitudes of the resulting oscillations are associated with the orientation of the telescope plane relative to the ecliptic. As in the circular restricted problem, initial conditions for the linearized equations must be selected so as not to excite the convergent or divergent linear modes. In a higher order analysis, the telescope relative motion equations include the effects of the position of the

Method for Grey Scale Mapping of Underground Obstacles Using Video Pulse Radar Return

DTIC Science & Technology

1978-12-01

the botton of Figure 3 is apparent. However, Figure 4 exhibits the one observed weakness of the Lagrange (or any polynomial) method. Large...t. . .. MWISS.S*4SW. - . W. . . SSV*V~t~fl~d*W*.StS . W ..... *W... *sn AW ~oo .*IB.Se.ve SWWZ.WWT...W. . . WVW.S* . *SS~SUA*.. ........ 0.305 mi stilf...5533 .. ... tel +lllt~llllllllllllllllll~l~ll............. . : .......lI~lI I llll~~ll .---I e~ ---- ..... lot I s 1 1i ....... IEr .5C

4. VISTA POINT AND INTERPRETIVE PLAQUE AT LEE VINING CANYON. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

4. VISTA POINT AND INTERPRETIVE PLAQUE AT LEE VINING CANYON. NOTE ROAD CUT ON CANYON WALL. LOOKING NNE. GIS: N-37 56 30.3 / 119 13 44.8 - Tioga Road, Between Crane Flat & Tioga Pass, Yosemite Village, Mariposa County, CA

A low diffusive Lagrange-remap scheme for the simulation of violent air-water free-surface flows

NASA Astrophysics Data System (ADS)

Bernard-Champmartin, Aude; De Vuyst, Florian

2014-10-01

In 2002, Després and Lagoutière [17] proposed a low-diffusive advection scheme for pure transport equation problems, which is particularly accurate for step-shaped solutions, and thus suited for interface tracking procedure by a color function. This has been extended by Kokh and Lagoutière [28] in the context of compressible multifluid flows using a five-equation model. In this paper, we explore a simplified variant approach for gas-liquid three-equation models. The Eulerian numerical scheme has two ingredients: a robust remapped Lagrange solver for the solution of the volume-averaged equations, and a low diffusive compressive scheme for the advection of the gas mass fraction. Numerical experiments show the performance of the computational approach on various flow reference problems: dam break, sloshing of a tank filled with water, water-water impact and finally a case of Rayleigh-Taylor instability. One of the advantages of the present interface capturing solver is its natural implementation on parallel processors or computers.

Proline derivatives in fruits of bergamot (Citrus bergamia Risso et Poit): presence of N-methyl-L-proline and 4-hydroxy-L-prolinebetaine.

PubMed

Servillo, Luigi; Giovane, Alfonso; Balestrieri, Maria Luisa; Cautela, Domenico; Castaldo, Domenico

2011-01-12

The content of proline and various compounds deriving from its metabolism (4-hydroxy-L-proline, N-methyl-L-proline, N,N-dimethylproline, and 4-hydroxy-L-prolinebetaine) was determined in fruits and seeds of Bergamot (Citrus bergamia Risso et Poit), growing in the Calabria region (South Italy). A HPLC-ESI-tandem mass spectrometry method, which allowed rapid determination of L-proline, 4-hydroxy-L-proline, N-methyl-L-proline, N,N-dimethylproline, and 4-hydroxy-L-prolinebetaine in juice and extracts of bergamot fruit with minimum sample preparation and short analysis time (about 10 min), is presented. Proline and 4-hydroxy-L-proline levels in the samples were also determined by HPLC analysis with fluorescence detection and the results compared to those obtained with HPLC-ESI-tandem mass spectrometry. For the first time, the presence of N-methyl-L-proline and 4-hydroxy-L-prolinebetaine in the fruits of a plant of the Citrus genus is reported.

Reviews on 1,4-naphthoquinones from Diospyros L.

PubMed

Nematollahi, Alireza; Aminimoghadamfarouj, Noushin; Wiart, Christophe

2012-01-01

The genus Diospyros is one of the most important sources of bioactive compounds, exclusively 1,4-naphthoquinones. The following information is an attempt to cover the developments in the biology and phytochemistry of 1,4-naphthoquinones isolated from this genus, as well as the studies done and the suggested mechanisms regarding their activities. During the past 60 years, many of these agents have been isolated from Diospyros L. Twelve considerable bioactive structures are reported in this review. The basic 1,4-naphthoquinone skeletons, on which a large number of studies have been done, are plumbagin and diospyrin. Today, the potential for development of leads from 1,4-naphthoquinones obtained from Diospyros L. is growing dramatically, mainly in the area of anticancer and antibacterial investigations. The data prepared and described here are intended to be served as a reference tool to the natural products and chemistry specialists in order to expand the rational drug design.

Posttest RELAP4 analysis of LOFT experiment L1-4

DOE Office of Scientific and Technical Information (OSTI.GOV)

Grush, W.H.; Holmstrom, H.L.O.

Results of posttest analysis of LOFT loss-of-coolant experiment L1-4 with the RELAP4 code are presented. The results are compared with the pretest prediction and the test data. Differences between the RELAP4 model used for this analysis and that used for the pretest prediction are in the areas of initial conditions, nodalization, emergency core cooling system, broken loop hot leg, and steam generator secondary. In general, these changes made only minor improvement in the comparison of the analytical results to the data. Also presented are the results of a limited study of LOFT downcomer modeling which compared the performance of themore » conventional single downcomer model with that of the new split downcomer model. A RELAP4 sensitivity calculation with artificially elevated emergency core coolant temperature was performed to highlight the need for an ECC mixing model in RELAP4.« less

Orbit Determination Accuracy for Comets on Earth-Impacting Trajectories

NASA Technical Reports Server (NTRS)

Kay-Bunnell, Linda

2004-01-01

The results presented show the level of orbit determination accuracy obtainable for long-period comets discovered approximately one year before collision with Earth. Preliminary orbits are determined from simulated observations using Gauss' method. Additional measurements are incorporated to improve the solution through the use of a Kalman filter, and include non-gravitational perturbations due to outgassing. Comparisons between observatories in several different circular heliocentric orbits show that observatories in orbits with radii less than 1 AU result in increased orbit determination accuracy for short tracking durations due to increased parallax per unit time. However, an observatory at 1 AU will perform similarly if the tracking duration is increased, and accuracy is significantly improved if additional observatories are positioned at the Sun-Earth Lagrange points L3, L4, or L5. A single observatory at 1 AU capable of both optical and range measurements yields the highest orbit determination accuracy in the shortest amount of time when compared to other systems of observatories.

Synoptic ozone, cloud reflectivity, and erythemal irradiance from sunrise to sunset for the whole earth as viewed by the DSCOVR spacecraft from the earth-sun Lagrange 1 orbit

NASA Astrophysics Data System (ADS)

Herman, Jay; Huang, Liang; McPeters, Richard; Ziemke, Jerry; Cede, Alexander; Blank, Karin

2018-01-01

EPIC (Earth Polychromatic Imaging Camera) on board the DSCOVR (Deep Space Climate Observatory) spacecraft is the first earth science instrument located near the earth-sun gravitational plus centrifugal force balance point, Lagrange 1. EPIC measures earth-reflected radiances in 10 wavelength channels ranging from 317.5 to 779.5 nm. Of these channels, four are in the UV range 317.5, 325, 340, and 388 nm, which are used to retrieve O3, 388 nm scene reflectivity (LER: Lambert equivalent reflectivity), SO2, and aerosol properties. These new synoptic quantities are retrieved for the entire sunlit globe from sunrise to sunset multiple times per day as the earth rotates in EPIC's field of view. Retrieved ozone amounts agree with ground-based measurements and satellite data to within 3 %. The ozone amounts and LER are combined to derive the erythemal irradiance for the earth's entire sunlit surface at a nadir resolution of 18 × 18 km2 using a computationally efficient approximation to a radiative transfer calculation of irradiance. The results show very high summertime values of the UV index (UVI) in the Andes and Himalayas (greater than 18), and high values of UVI near the Equator at equinox.

Computational analysis of human and mouse CREB3L4 Protein

PubMed Central

Velpula, Kiran Kumar; Rehman, Azeem Abdul; Chigurupati, Soumya; Sanam, Ramadevi; Inampudi, Krishna Kishore; Akila, Chandra Sekhar

2012-01-01

CREB3L4 is a member of the CREB/ATF transcription factor family, characterized by their regulation of gene expression through the cAMP-responsive element. Previous studies identified this protein in mice and humans. Whereas CREB3L4 in mice (referred to as Tisp40) is found in the testes and functions in spermatogenesis, human CREB3L4 is primarily detected in the prostate and has been implicated in cancer. We conducted computational analyses to compare the structural homology between murine Tisp40α human CREB3L4. Our results reveal that the primary and secondary structures of the two proteins contain high similarity. Additionally, predicted helical transmembrane structure reveals that the proteins likely have similar structure and function. This study offers preliminary findings that support the translation of mouse Tisp40α findings into human models, based on structural homology. PMID:22829733

Synthesis and analysis of 2-[211At]-L-phenylalanine and 4-[211At]-L-phenylalanine and their uptake in human glioma cell cultures in-vitro.

PubMed

Meyer, Geerd J; Walte, Almut; Sriyapureddy, Siva R; Grote, Michaela; Krull, Doris; Korkmaz, Zekiye; Knapp, Wolfram H

2010-06-01

2-[211At]-L-phenylalanine and 4-[211At]-L-phenylalanine were prepared from the corresponding iodo and bromo derivatives using the Cu(+)-assisted nucleophilic exchange. 4-[211At]-L-phenylalanine was additionally prepared by destannylation of the BOC-derivatized 4-tributylstannyl-L-phenylalanine. Radiochemical yields of 2-[211At]-L-phenylalanine and 4-[211At]-L-phenylalanine by nucleophilic exchange were 52-74% and 65-85%. Radiochemical yield of 4-[211At]-L-phenylalanine by electrophilic destannylation was 35-50%. HPLC sequence analysis showed that 2-[211At]-L-phenylalanine followed the halogen sequence (F4-[211At]-L-phenylalanine eluted between 4-Br-L-phenylalanine and 4-I-L-phenylalanine (F4-[211At]-L-phenylalanine and 4-[131I]-L-phenylalanine in DBTRG-05MG glioma cells was inhibited by l-phenylalanine 7-fold and 6-fold, respectively. Copyright 2010 Elsevier Ltd. All rights reserved.

14. 'ANNISQUAM POINT JAN. 4, 1898.' Photocopy of photograph ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

14. 'ANNISQUAM POINT -- JAN. 4, 1898.' Photocopy of photograph (original glass plate negative #T86 in the collection of the Annisquam Historical Society, Annisquam, Massachusetts). Photographer: Martha Harvey (1862-1949). (The handwritten legend along the top edge of the photograph is scratched in the emulsion of the original glass plate negative. Consequently it reads in reverse when printed.) - Annisquam Bridge, Spanning Lobster Cove between Washington & River Streets, Gloucester, Essex County, MA

The E3 ubiquitin ligase Nedd4/Nedd4L is directly regulated by microRNA 1

PubMed Central

Heidersbach, Amy; Kathiriya, Irfan S.; Garay, Bayardo I.; Ivey, Kathryn N.

2017-01-01

miR-1 is a small noncoding RNA molecule that modulates gene expression in heart and skeletal muscle. Loss of Drosophila miR-1 produces defects in somatic muscle and embryonic heart development, which have been partly attributed to miR-1 directly targeting Delta to decrease Notch signaling. Here, we show that overexpression of miR-1 in the fly wing can paradoxically increase Notch activity independently of its effects on Delta. Analyses of potential miR-1 targets revealed that miR-1 directly regulates the 3′UTR of the E3 ubiquitin ligase Nedd4. Analysis of embryonic and adult fly heart revealed that the Nedd4 protein regulates heart development in Drosophila. Larval fly hearts overexpressing miR-1 have profound defects in actin filament organization that are partially rescued by concurrent overexpression of Nedd4. These results indicate that miR-1 and Nedd4 act together in the formation and actin-dependent patterning of the fly heart. Importantly, we have found that the biochemical and genetic relationship between miR-1 and the mammalian ortholog Nedd4-like (Nedd4l) is evolutionarily conserved in the mammalian heart, potentially indicating a role for Nedd4L in mammalian postnatal maturation. Thus, miR-1-mediated regulation of Nedd4/Nedd4L expression may serve to broadly modulate the trafficking or degradation of Nedd4/Nedd4L substrates in the heart. PMID:28246214

On the stability of triangular points in the relativistic R3BP when the bigger primary is oblate and the smaller one radiating with application on Cen X-4 binary system

NASA Astrophysics Data System (ADS)

Bello, Nakone; Umar, Aishetu

2018-06-01

In the framework of the relativistic R3BP, we examine the effects of oblateness of the primary body and radiation pressure of the secondary on the positions and stability of the triangular points L4,5. It is found that the parameters involved all affect the positions and increase in any of the parameters leads to a reduction in the size of the region of stability. Thus establishing their destabilizing tendencies. The presence of positive real roots or positive real part in complex roots affirms the instability of L4,5 of the problem when applied to Cen X-4.

Four-point bending as a method for quantitatively evaluating spinal arthrodesis in a rat model.

PubMed

Robinson, Samuel T; Svet, Mark T; Kanim, Linda A; Metzger, Melodie F

2015-02-01

The most common method of evaluating the success (or failure) of rat spinal fusion procedures is manual palpation testing. Whereas manual palpation provides only a subjective binary answer (fused or not fused) regarding the success of a fusion surgery, mechanical testing can provide more quantitative data by assessing variations in strength among treatment groups. We here describe a mechanical testing method to quantitatively assess single-level spinal fusion in a rat model, to improve on the binary and subjective nature of manual palpation as an end point for fusion-related studies. We tested explanted lumbar segments from Sprague-Dawley rat spines after single-level posterolateral fusion procedures at L4-L5. Segments were classified as 'not fused,' 'restricted motion,' or 'fused' by using manual palpation testing. After thorough dissection and potting of the spine, 4-point bending in flexion then was applied to the L4-L5 motion segment, and stiffness was measured as the slope of the moment-displacement curve. Results demonstrated statistically significant differences in stiffness among all groups, which were consistent with preliminary grading according to manual palpation. In addition, the 4-point bending results provided quantitative information regarding the quality of the bony union formed and therefore enabled the comparison of fused specimens. Our results demonstrate that 4-point bending is a simple, reliable, and effective way to describe and compare results among rat spines after fusion surgery.

Double-pulse dichromatic photolysis of fac-CIRe(CO){sub 3}L{sub 2} (L=4-phenylpyridine or 4-cyanopyridine): Photohomolysis of Re-L bonds insuced by irradiation of photochemically unreactive charge transfer states

DOE Office of Scientific and Technical Information (OSTI.GOV)

Feliz, M.; Ferraudi, G.

1992-04-02

Photochemical reactions of fac-ClRe(CO){sub 3}L{sub 2} (L=4-phenylpyridine or 4-cyanopyridine), were investigated by sequential biphotonic excitations: one laser flash was used for the preparation of the compounds in the lowest lying MLCT (Re{r_arrow}) state and another flash for the irradiation of the compounds in such excited states. These photolyses led to photodecompostions into CIRe(CO){sub 3}L{sup +} and L{sup .} in a charge transfer state placed 40 Kk above ground state. Quantum yields determined or various excitation energies show that not all the excited state populated in monophotonic excitations can be reached under the sequential biphotonic regime. Therefore, photogeneration of the biradicalmore » intermediate, ClRe(CO){sub 3}L{sup +} and L{sup .}, from ligand-centered states has not been detected in these experiments. Results from monophotonic and biphotonic excitations have been used for a semiquantitative mapping of the excited-state potential surfaces. 41 refs., 6 figs.« less

Sequence of the amino-terminal region of rat liver ribosomal proteins S4, S6, S8, L6, L7a, L18, L27, L30, L37, L37a, and L39.

PubMed

Wittmann-Liebold, B; Geissler, A W; Lin, A; Wool, I G

1979-01-01

The sequence of the amino-terminal region of eleven rat liver ribosomal proteins--S4, S6, S8, L6, L7a, L18, L27, L30, L37a, and L39--was determined. The analysis confirmed the homogeneity of the proteins and suggests that they are unique, since no extensive common sequences were found. The N-terminal regions of the rat liver proteins were compared with amino acid sequences in Saccharomyces cerevisiae and in Escherichia coli ribosomal proteins. It seems likely that the proteins L37 from rat liver and Y55 from yeast ribosomes are homologous. It is possible that rat liver L7a or L37a or both are related to S cerevisiae Y44, although the similar sequences are at the amino-terminus of the rat liver proteins and in an internal region of Y44. A number of similarities in the sequences of rat liver and E coli ribosomal proteins have been found; however, it is not yet possible to say whether they connote a common ancestry.

Axial point groups: rank 1, 2, 3 and 4 property tensor tables.

PubMed

Litvin, Daniel B

2015-05-01

The form of a physical property tensor of a quasi-one-dimensional material such as a nanotube or a polymer is determined from the material's axial point group. Tables of the form of rank 1, 2, 3 and 4 property tensors are presented for a wide variety of magnetic and non-magnetic tensor types invariant under each point group in all 31 infinite series of axial point groups. An application of these tables is given in the prediction of the net polarization and magnetic-field-induced polarization in a one-dimensional longitudinal conical magnetic structure in multiferroic hexaferrites.

The COOH-terminal peptide of platelet factor-4 variant (CXCL4L1/PF-4var47-70) strongly inhibits angiogenesis and suppresses B16 melanoma growth in vivo.

PubMed

Vandercappellen, Jo; Liekens, Sandra; Bronckaers, Annelies; Noppen, Samuel; Ronsse, Isabelle; Dillen, Chris; Belleri, Mirella; Mitola, Stefania; Proost, Paul; Presta, Marco; Struyf, Sofie; Van Damme, Jo

2010-03-01

Chemokines influence tumor growth directly or indirectly via both angiogenesis and tumor-leukocyte interactions. Platelet factor-4 (CXCL4/PF-4), which is released from alpha-granules of activated platelets, is the first described angiostatic chemokine. Recently, it was found that the variant of CXCL4/PF-4 (CXCL4L1/PF-4var) could exert a more pronounced angiostatic and antitumoral effect than CXCL4/PF-4. However, the molecular mechanisms of the angiostatic activities of the PF-4 forms remain partially elusive. Here, we studied the biological properties of the chemically synthesized COOH-terminal peptides of CXCL4/PF-4 (CXCL4/PF-4(47-70)) and CXCL4L1/PF-4var (CXCL4L1/PF-4var(47-70)). Both PF-4 peptides lacked monocyte and lymphocyte chemotactic activity but equally well inhibited (25 nmol/L) endothelial cell motility and proliferation in the presence of a single stimulus (i.e., exogenous recombinant fibroblast growth factor-2). In contrast, when assayed in more complex angiogenesis test systems characterized by the presence of multiple mediators, including in vitro wound-healing (2.5 nmol/L versus 12.5 nmol/L), Matrigel (60 nmol/L versus 300 nmol/L), and chorioallantoic membrane assays, CXCL4L1/PF-4var(47-70) was found to be significantly (5-fold) more angiostatic than CXCL4/PF-4(47-70). In addition, low (7 microg total) doses of intratumoral CXCL4L1/PF-4var(47-70) inhibited B16 melanoma growth in mice more extensively than CXCL4/PF-4(47-70). This antitumoral activity was predominantly mediated through inhibition of angiogenesis (without affecting blood vessel stability) and induction of apoptosis, as evidenced by immunohistochemical and fluorescent staining of B16 tumor tissue. In conclusion, CXCL4L1/PF-4var(47-70) is a potent antitumoral and antiangiogenic peptide. These results may represent the basis for the design of CXCL4L1/PF-4var COOH-terminal-derived peptidomimetic anticancer drugs.

Novel Enzyme Family Found in Filamentous Fungi Catalyzing trans-4-Hydroxylation of l-Pipecolic Acid

PubMed Central

Hibi, Makoto; Mori, Ryosuke; Miyake, Ryoma; Kawabata, Hiroshi; Kozono, Shoko; Takahashi, Satomi

2016-01-01

Hydroxypipecolic acids are bioactive compounds widely distributed in nature and are valuable building blocks for the organic synthesis of pharmaceuticals. We have found a novel hydroxylating enzyme with activity toward l-pipecolic acid (l-Pip) in a filamentous fungus, Fusarium oxysporum c8D. The enzyme l-Pip trans-4-hydroxylase (Pip4H) of F. oxysporum (FoPip4H) belongs to the Fe(II)/α-ketoglutarate-dependent dioxygenase superfamily, catalyzes the regio- and stereoselective hydroxylation of l-Pip, and produces optically pure trans-4-hydroxy-l-pipecolic acid (trans-4-l-HyPip). Amino acid sequence analysis revealed several fungal enzymes homologous with FoPip4H, and five of these also had l-Pip trans-4-hydroxylation activity. In particular, the homologous Pip4H enzyme derived from Aspergillus nidulans FGSC A4 (AnPip4H) had a broader substrate specificity spectrum than other homologues and reacted with the l and d forms of various cyclic and aliphatic amino acids. Using FoPip4H as a biocatalyst, a system for the preparative-scale production of chiral trans-4-l-HyPip was successfully developed. Thus, we report a fungal family of l-Pip hydroxylases and the enzymatic preparation of trans-4-l-HyPip, a bioactive compound and a constituent of secondary metabolites with useful physiological activities. PMID:26801577

Point-point and point-line moving-window correlation spectroscopy and its applications

NASA Astrophysics Data System (ADS)

Zhou, Qun; Sun, Suqin; Zhan, Daqi; Yu, Zhiwu

2008-07-01

In this paper, we present a new extension of generalized two-dimensional (2D) correlation spectroscopy. Two new algorithms, namely point-point (P-P) correlation and point-line (P-L) correlation, have been introduced to do the moving-window 2D correlation (MW2D) analysis. The new method has been applied to a spectral model consisting of two different processes. The results indicate that P-P correlation spectroscopy can unveil the details and re-constitute the entire process, whilst the P-L can provide general feature of the concerned processes. Phase transition behavior of dimyristoylphosphotidylethanolamine (DMPE) has been studied using MW2D correlation spectroscopy. The newly proposed method verifies that the phase transition temperature is 56 °C, same as the result got from a differential scanning calorimeter. To illustrate the new method further, a lysine and lactose mixture has been studied under thermo perturbation. Using the P-P MW2D, the Maillard reaction of the mixture was clearly monitored, which has been very difficult using conventional display of FTIR spectra.

Synthesis of L-threo-3,4-dihydroxyphenylserine (L-threo-DOPS) with thermostabilized low-specific L-threonine aldolase from Streptomyces coelicolor A3(2).

PubMed

Balk, Sang-Ho; Yoshioka, Hideki; Yukawa, Hideaki; Harayama, Shigeaki

2007-05-01

Stability-enhanced mutants, H44, 11-94, 5A2-84, and F8, of L-threonine aldolase (L-TA) from Streptomyces coelicolor A3(2) (SCO1085) were isolated by an error-prone PCR followed by a high-throughput screening. Each of these mutant, had a single amino acid substitution: H177Y in the H44 mutant, A169T in the 11-94 mutant, D104N in the 5A2-84 mutant and Fl81 in the F8 mutant. The residual L-TA activity of the wild-type L-TA after a heat treatment for 20 min at 60 degrees C was only 10.6%. However, those in the stability-enhanced mutants were 85.7% for the H44 mutant, 58.6% for the F8 mutant, 62.1% for the 5A2-84 mutant, and 67.6% for the 11-94 mutant. Although the half-life of the wild-type L-TA at 63 degrees C was 1.3 min, those of the mutant L-TAs were longer: 14.6 min for the H44 mutant, 3.7 min for the 11-94 mutant, 5.8 min for the 5A2-84 mutant, and 5.0 min for the F8 mutant. The specific activity did not change in most of the mutants, but it was decreased by 45% in the case of mutant F8. When the aldol condensation of glycine and 3,4-dihydroxybenzaldehyde was studied by using whole cells of Escherichia coli containing the wild-type L-TA gene, L-threo-3,4-dihydroxyphenylserine (L.-threo-DOPS) was successfully synthesized with a yield of 2.0 mg/ml after 20 repeated batch reactions for 100 h. However, the L-threo-DOPS synthesizing activity of the enzyme decreased with increased cycles of the batch reactions. Compared with the wild-type L-TA, H44 L-TA kept its L-threo-DOPS synthesizing activity almost constant during the 20 repeated batch reactions for 100 h, yielding 4.0 mg/ml of L-threo-DOPS. This result showed that H44 L-TA is more effective than the wild-type L-TA for the mass production of L-threo-DOPS.

Transition from the mechanics of material points to the mechanics of structured particles

NASA Astrophysics Data System (ADS)

Somsikov, V. M.

2016-01-01

In this paper, necessity of creation of mechanics of structured particles is discussed. The way to create this mechanics within the laws of classical mechanics with the use of energy equation is shown. The occurrence of breaking of time symmetry within the mechanics of structured particles is shown, as well as the introduction of concept of entropy in the framework of classical mechanics. The way to create the mechanics of non-equilibrium systems in the thermodynamic approach is shown. It is also shown that the use of hypothesis of holonomic constraints while deriving the canonical Lagrange equation made it impossible to describe irreversible dynamics. The difference between the mechanics of structured particles and the mechanics of material points is discussed. It is also shown that the matter is infinitely divisible according to the laws of classical mechanics.

Carbon Dioxide Compensation Points of Flowering Plants 1

PubMed Central

Krenzer, Eugene G.; Moss, Dale N.; Crookston, R. Kent

1975-01-01

Carbon dioxide compensation points of several hundred species of monocotyledons and dicotyledons have been measured during the course of various experiments in our laboratory over a period of several years. These have been classified into two groups: high, compensation points of 40 μl/l or greater; and low, compensation points of 10 μl/l or less. They are listed alphabetically both by families and species for monocotyledons and dicotyledons. Only two species did not unequivocally fit into the above established groups. These were Moricandia arvensis (L.) DC., which had an average compensation point of 26 μl/l and Panicum milioides Nees ex Trin., which was variable, but most often equilibrated between 12 to 20 μl/l CO2. PMID:16659272

Optimized Hypernetted-Chain Solutions for Helium -4 Surfaces and Metal Surfaces

NASA Astrophysics Data System (ADS)

Qian, Guo-Xin

This thesis is a study of inhomogeneous Bose systems such as liquid ('4)He slabs and inhomogeneous Fermi systems such as the electron gas in metal films, at zero temperature. Using a Jastrow-type many-body wavefunction, the ground state energy is expressed by means of Bogoliubov-Born-Green-Kirkwood -Yvon and Hypernetted-Chain techniques. For Bose systems, Euler-Lagrange equations are derived for the one- and two -body functions and systematic approximation methods are physically motivated. It is shown that the optimized variational method includes a self-consistent summation of ladder- and ring-diagrams of conventional many-body theory. For Fermi systems, a linear potential model is adopted to generate the optimized Hartree-Fock basis. Euler-Lagrange equations are derived for the two-body correlations which serve to screen the strong bare Coulomb interaction. The optimization of the pair correlation leads to an expression of correlation energy in which the state averaged RPA part is separated. Numerical applications are presented for the density profile and pair distribution function for both ('4)He surfaces and metal surfaces. Both the bulk and surface energies are calculated in good agreement with experiments.

Town Hall Meeting Presentation

NASA Technical Reports Server (NTRS)

Mather, John C.

2002-01-01

The James Webb Space Telescope (JWST), formerly known as the Next Generation Space Telescope (NGST), will be the successor to the Hubble Space Telescope. It will carry 3 instruments to a deep space orbit around the Sun-Earth Lagrange point L2, and will cover the wavelength range from 0.6 to 28 microns. The design concepts and current status of the project will be summarized, including the telescope and observatory contract proposed by the new prime contractor, TRW.

26 CFR 36.3121(l)(10)-4 - Payment of amounts equivalent to tax.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 26 Internal Revenue 15 2010-04-01 2010-04-01 false Payment of amounts equivalent to tax. 36.3121(l)(10)-4 Section 36.3121(l)(10)-4 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY... SUBSIDIARIES § 36.3121(l)(10)-4 Payment of amounts equivalent to tax. A domestic corporation which has entered...

Dynamics of tethered satellites in the vicinity of the Lagrangian point L2 of the Earth-Moon system

NASA Astrophysics Data System (ADS)

Baião, M. F.; Stuchi, T. J.

2017-08-01

This paper analyzes the dynamical evolution of satellites formed by two masses connected by a cable— tethered satellites. We derive the Lagrangian equations of motion in the neighborhood of the collinear equilibrium points, especially for the L2 , of the restricted problem of three bodies. The rigid body configuration is expanded in Legendre polynomials up to fourth degree. We present some numerical simulations of the influence of the parameters such as cable length, mass ratio and initial conditions in the behavior of the tethered satellites. The equation for the collinear equilibrium point is derived and numerically solved. The evolution of the equilibria with the variation of the cable length as a parameter is studied. We also present a discussion of the linear stability around these equilibria. Based on this analysis calculate some unstable Lyapunov orbits associated to these equilibrium points. We found periodic orbits in which the tether travels parallel to itself without involving the angular motion. The numerical applications are focused on the Earth-Moon system. However, the general character of the equations allows applications to the L1 equilibrium and obviously to systems other than the Earth-Moon.

Spontaneous T-cell responses against the immune check point programmed-death-ligand 1 (PD-L1) in patients with chronic myeloproliferative neoplasms correlate with disease stage and clinical response.

PubMed

Holmström, Morten Orebo; Riley, Caroline Hasselbalch; Skov, Vibe; Svane, Inge Marie; Hasselbalch, Hans Carl; Andersen, Mads Hald

2018-01-01

The Chronic Myeloproliferative Neoplasms (MPN) are cancers characterized by hyperinflammation and immune deregulation. Concurrently, the expression of the immune check point programmed death ligand 1 (PD-L1) is induced by inflammation. In this study we report on the occurrence of spontaneous T cell responses against a PD-L1 derived epitope in patients with MPN. We show that 71% of patients display a significant immune response against PD-L1, and patients with advanced MPN have significantly fewer and weaker PD-L1 specific immune responses compared to patients with non-advanced MPN. The PD-L1 specific T cell responses are CD4 + T cell responses, and by gene expression analysis we show that expression of PD-L1 is enhanced in patients with MPN. This could imply that the tumor specific immune response in MPN could be enhanced by vaccination with PD-L1 derived epitopes by boosting the anti-regulatory immune response hereby allowing tumor specific T cell to exert anti-tumor immunity.

Multistage ordering and critical singularities in C o1 -xZ nxA l2O4(0 ≤x ≤1 ) : Dilution and pressure effects in a magnetically frustrated system

NASA Astrophysics Data System (ADS)

Naka, Takashi; Sato, Koichi; Matsushita, Yoshitaka; Terada, Noriki; Ishii, Satoshi; Nakane, Takayuki; Taguchi, Minori; Nakayama, Minako; Hashishin, Takeshi; Ohara, Satoshi; Takami, Seiichi; Matsushita, Akiyuki

2015-06-01

We report comprehensive studies of the crystallographic, magnetic, and thermal properties of a spinel-type magnetically frustrated compound, CoA l2O4 , and a magnetically diluted system, C o1- xZ nxA l2O4 . These studies revealed the effects of dilution and disorder when the tetrahedral magnetic Co ion was replaced by the nonmagnetic Zn ion. Low-temperature anomalies were observed in magnetic susceptibility at x <0.6 . A multicritical point was apparent at T =3.4 K and x =0.12 , where the antiferromagnetic, spin-glass-like, and paramagnetic phases met. At that point, the quenched ferromagnetic component induced by a magnetic field during cooling was sharply enhanced and was observable below x =0.6 . At x ˜0.6 , magnetic susceptibility and specific heat were described by temperature power laws, χ ˜C /T ˜T-δ , in accord with the site percolation threshold of the diamond lattice. This behavior is reminiscent of a quantum critical singularity. We propose an x -temperature phase diagram in the range 0 ≤x ≤1 for C o1- xZ nxA l2O4 . The transition temperature of CoA l2O4 determined from magnetic susceptibility measured under hydrostatic pressure increased with increasing pressure.

The role of the CXC chemokines platelet factor-4 (CXCL4/PF-4) and its variant (CXCL4L1/PF-4var) in inflammation, angiogenesis and cancer.

PubMed

Vandercappellen, Jo; Van Damme, Jo; Struyf, Sofie

2011-02-01

Chemokines are chemotactic cytokines which recruit leukocytes to inflammatory sites. They also affect tumor development and metastasis by acting as growth factor, by attracting pro- or anti-tumoral leukocytes or by influencing angiogenesis. Platelet factor-4 (CXCL4/PF-4) was the first chemokine shown to inhibit angiogenesis. CXCL4L1/PF-4var, recently isolated from thrombin-stimulated platelets, differing from authentic CXCL4/PF-4 in three carboxy-terminally located amino acids, was found to be more potent than CXCL4/PF-4 in inhibiting angiogenesis and tumor growth. Both glycosaminoglycans (GAG) and CXCR3 are implicated in the activities of the PF-4 variants. This report reviews the current knowledge on the role of CXCL4/PF-4 and CXCL4L1/PF-4var in physiological and pathological processes. In particular, the role of CXCL4/PF-4 in cancer, heparin-induced thrombocytopenia and atherosclerosis is described. Copyright © 2010 Elsevier Ltd. All rights reserved.

SiC-CMC-Zircaloy-4 Nuclear Fuel Cladding Performance during 4-Point Tubular Bend Testing

DOE Office of Scientific and Technical Information (OSTI.GOV)

IJ van Rooyen; WR Lloyd; TL Trowbridge

2013-09-01

The U.S. Department of Energy Office of Nuclear Energy (DOE NE) established the Light Water Reactor Sustainability (LWRS) program to develop technologies and other solutions to improve the reliability, sustain the safety, and extend the life of current reactors. The Advanced LWR Nuclear Fuel Development Pathway in the LWRS program encompasses strategic research focused on improving reactor core economics and safety margins through the development of an advanced fuel cladding system. Recent investigations of potential options for “accident tolerant” nuclear fuel systems point to the potential benefits of silicon carbide (SiC) cladding. One of the proposed SiC-based fuel cladding designsmore » being investigated incorporates a SiC ceramic matrix composite (CMC) as a structural material supplementing an internal Zircaloy-4 (Zr-4) liner tube, referred to as the hybrid clad design. Characterization of the advanced cladding designs will include a number of out-of-pile (nonnuclear) tests, followed by in-pile irradiation testing of the most promising designs. One of the out-of-pile characterization tests provides measurement of the mechanical properties of the cladding tube using four point bend testing. Although the material properties of the different subsystems (materials) will be determined separately, in this paper we present results of 4-point bending tests performed on fully assembled hybrid cladding tube mock-ups, an assembled Zr-4 cladding tube mock-up as a standard and initial testing results on bare SiC-CMC sleeves to assist in defining design parameters. The hybrid mock-up samples incorporated SiC-CMC sleeves fabricated with 7 polymer impregnation and pyrolysis (PIP) cycles. To provide comparative information; both 1- and 2-ply braided SiC-CMC sleeves were used in this development study. Preliminary stress simulations were performed using the BISON nuclear fuel performance code to show the stress distribution differences for varying lengths between

75 FR 4426 - Florida Power and Light Company; Turkey Point Nuclear Generating Units 3 and 4; Environmental...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-01-27

... Light Company; Turkey Point Nuclear Generating Units 3 and 4; Environmental Assessment and Finding of No... (the licensee), for operation of the Turkey Point Units 3 and 4, located in Miami, Florida. In...-ductility temperature) of the Linde 80 weld materials present in the beltline region of the Turkey Point...

Lagrange constraint neural networks for massive pixel parallel image demixing

NASA Astrophysics Data System (ADS)

Szu, Harold H.; Hsu, Charles C.

2002-03-01

We have shown that the remote sensing optical imaging to achieve detailed sub-pixel decomposition is a unique application of blind source separation (BSS) that is truly linear of far away weak signal, instantaneous speed of light without delay, and along the line of sight without multiple paths. In early papers, we have presented a direct application of statistical mechanical de-mixing method called Lagrange Constraint Neural Network (LCNN). While the BSAO algorithm (using a posteriori MaxEnt ANN and neighborhood pixel average) is not acceptable for remote sensing, a mirror symmetric LCNN approach is all right assuming a priori MaxEnt for unknown sources to be averaged over the source statistics (not neighborhood pixel data) in a pixel-by-pixel independent fashion. LCNN reduces the computation complexity, save a great number of memory devices, and cut the cost of implementation. The Landsat system is designed to measure the radiation to deduce surface conditions and materials. For any given material, the amount of emitted and reflected radiation varies by the wavelength. In practice, a single pixel of a Landsat image has seven channels receiving 0.1 to 12 microns of radiation from the ground within a 20x20 meter footprint containing a variety of radiation materials. A-priori LCNN algorithm provides the spatial-temporal variation of mixture that is hardly de-mixable by other a-posteriori BSS or ICA methods. We have already compared the Landsat remote sensing using both methods in WCCI 2002 Hawaii. Unfortunately the absolute benchmark is not possible because of lacking of the ground truth. We will arbitrarily mix two incoherent sampled images as the ground truth. However, the constant total probability of co-located sources within the pixel footprint is necessary for the remote sensing constraint (since on a clear day the total reflecting energy is constant in neighborhood receiving pixel sensors), we have to normalized two image pixel-by-pixel as well. Then, the

The research of the coupled orbital-attitude controlled motion of celestial body in the neighborhood of the collinear libration point L1

NASA Astrophysics Data System (ADS)

Shmyrov, A.; Shmyrov, V.; Shymanchuk, D.

2017-10-01

This article considers the motion of a celestial body within the restricted three-body problem of the Sun-Earth system. The equations of controlled coupled attitude-orbit motion in the neighborhood of collinear libration point L1 are investigated. The translational orbital motion of a celestial body is described using Hill's equations of circular restricted three-body problem of the Sun-Earth system. Rotational orbital motion is described using Euler's dynamic equations and quaternion kinematic equation. We investigate the problem of stability of celestial body rotational orbital motion in relative equilibrium positions and stabilization of celestial body rotational orbital motion with proposed control laws in the neighborhood of collinear libration point L1. To study stabilization problem, Lyapunov function is constructed in the form of the sum of the kinetic energy and special "kinematic function" of the Rodriguez-Hamiltonian parameters. Numerical modeling of the controlled rotational motion of a celestial body at libration point L1 is carried out. The numerical characteristics of the control parameters and rotational motion are given.

Modelling of Dynamics of a Wheeled Mobile Robot with Mecanum Wheels with the use of Lagrange Equations of the Second Kind

NASA Astrophysics Data System (ADS)

Hendzel, Z.; Rykała, Ł.

2017-02-01

The work presents the dynamic equations of motion of a wheeled mobile robot with mecanum wheels derived with the use of Lagrange equations of the second kind. Mecanum wheels are a new type of wheels used in wheeled mobile robots and they consist of freely rotating rollers attached to the circumference of the wheels. In order to derive dynamic equations of motion of a wheeled mobile robot, the kinetic energy of the system is determined, as well as the generalised forces affecting the system. The resulting mathematical model of a wheeled mobile robot was generated with the use of Maple V software. The results of a solution of inverse and forward problems of dynamics of the discussed object are also published.

Consistency of L4 TM absolute calibration with respect to the L5 TM sensor based on near-simultaneous image acquisition

NASA Astrophysics Data System (ADS)

Chander, Gyanesh; Helder, Dennis L.; Malla, Rimy; Micijevic, Esad; Mettler, Cory J.

2007-09-01

The Landsat archive provides more than 35 years of uninterrupted multispectral remotely sensed data of Earth observations. Since 1972, Landsat missions have carried different types of sensors, from the Return Beam Vidicon (RBV) camera to the Enhanced Thematic Mapper Plus (ETM+). However, the Thematic Mapper (TM) sensors on Landsat 4 (L4) and Landsat 5 (L5), launched in 1982 and 1984 respectively, are the backbone of an extensive archive. Effective April 2, 2007, the radiometric calibration of L5 TM data processed and distributed by the U.S. Geological Survey (USGS) Center for Earth Resources Observation and Science (EROS) was updated to use an improved lifetime gain model, based on the instrument's detector response to pseudo-invariant desert site data and cross-calibration with the L7 ETM+. However, no modifications were ever made to the radiometric calibration procedure of the Landsat 4 (L4) TM data. The L4 TM radiometric calibration procedure has continued to use the Internal Calibrator (IC) based calibration algorithms and the post calibration dynamic ranges, as previously defined. To evaluate the "current" absolute accuracy of these two sensors, image pairs from the L5 TM and L4 TM sensors were compared. The number of coincident image pairs in the USGS EROS archive is limited, so the scene selection for the cross-calibration studies proved to be a challenge. Additionally, because of the lack of near-simultaneous images available over well-characterized and traditionally used calibration sites, alternate sites that have high reflectance, large dynamic range, high spatial uniformity, high sun elevation, and minimal cloud cover were investigated. The alternate sites were identified in Yuma, Iraq, Egypt, Libya, and Algeria. The cross-calibration approach involved comparing image statistics derived from large common areas observed eight days apart by the two sensors. This paper summarizes the average percent differences in reflectance estimates obtained between the

Consistency of L4 TM absolute calibration with respect to the L5 TM sensor based on near-simultaneous image acquisition

USGS Publications Warehouse

Chander, G.; Helder, D.L.; Malla, R.; Micijevic, E.; Mettler, C.J.

2007-01-01

The Landsat archive provides more than 35 years of uninterrupted multispectral remotely sensed data of Earth observations. Since 1972, Landsat missions have carried different types of sensors, from the Return Beam Vidicon (RBV) camera to the Enhanced Thematic Mapper Plus (ETM+). However, the Thematic Mapper (TM) sensors on Landsat 4 (L4) and Landsat 5 (L5), launched in 1982 and 1984 respectively, are the backbone of an extensive archive. Effective April 2, 2007, the radiometric calibration of L5 TM data processed and distributed by the U.S. Geological Survey (USGS) Center for Earth Resources Observation and Science (EROS) was updated to use an improved lifetime gain model, based on the instrument's detector response to pseudo-invariant desert site data and cross-calibration with the L7 ETM+. However, no modifications were ever made to the radiometric calibration procedure of the Landsat 4 (L4) TM data. The L4 TM radiometric calibration procedure has continued to use the Internal Calibrator (IC) based calibration algorithms and the post calibration dynamic ranges, as previously defined. To evaluate the "current" absolute accuracy of these two sensors, image pairs from the L5 TM and L4 TM sensors were compared. The number of coincident image pairs in the USGS EROS archive is limited, so the scene selection for the cross-calibration studies proved to be a challenge. Additionally, because of the lack of near-simultaneous images available over well-characterized and traditionally used calibration sites, alternate sites that have high reflectance, large dynamic range, high spatial uniformity, high sun elevation, and minimal cloud cover were investigated. The alternate sites were identified in Yuma, Iraq, Egypt, Libya, and Algeria. The cross-calibration approach involved comparing image statistics derived from large common areas observed eight days apart by the two sensors. This paper summarizes the average percent differences in reflectance estimates obtained between the

An Epha4/Sipa1l3/Wnt pathway regulates eye development and lens maturation.

PubMed

Rothe, Melanie; Kanwal, Noreen; Dietmann, Petra; Seigfried, Franziska A; Hempel, Annemarie; Schütz, Desiree; Reim, Dominik; Engels, Rebecca; Linnemann, Alexander; Schmeisser, Michael J; Bockmann, Juergen; Kühl, Michael; Boeckers, Tobias M; Kühl, Susanne J

2017-01-15

The signal-induced proliferation-associated family of proteins comprises four members, SIPA1 and SIPA1L1-3. Mutations of the human SIPA1L3 gene result in congenital cataracts. In Xenopus, loss of Sipa1l3 function led to a severe eye phenotype that was distinguished by smaller eyes and lenses including lens fiber cell maturation defects. We found a direct interaction between Sipa1l3 and Epha4, building a functional platform for proper ocular development. Epha4 deficiency phenocopied loss of Sipa1l3 and rescue experiments demonstrated that Epha4 acts upstream of Sipa1l3 during eye development, with both Sipa1l3 and Epha4 required for early eye specification. The ocular phenotype, upon loss of either Epha4 or Sipa1l3, was partially mediated by rax We demonstrate that canonical Wnt signaling is inhibited downstream of Epha4 and Sipa1l3 during normal eye development. Depletion of either Sipa1l3 or Epha4 resulted in an upregulation of axin2 expression, a direct Wnt/β-catenin target gene. In line with this, Sipa1l3 or Epha4 depletion could be rescued by blocking Wnt/β-catenin or activating non-canonical Wnt signaling. We therefore conclude that this pathomechanism prevents proper eye development and maturation of lens fiber cells, resulting in congenital cataracts. © 2017. Published by The Company of Biologists Ltd.

4. CONTROL BUILDING B, VIEW TOWARDS SOUTHEAST. Glenn L. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

4. CONTROL BUILDING B, VIEW TOWARDS SOUTHEAST. - Glenn L. Martin Company, Titan Missile Test Facilities, Control Building B, Waterton Canyon Road & Colorado Highway 121, Lakewood, Jefferson County, CO

Keeping a Spacecraft on the Sun-Earth Line

NASA Technical Reports Server (NTRS)

Roithmayr, Carlos M.; Kay-Bunnell, Linda

2005-01-01

Measurements of Earth's atmosphere as it occults sunlight can be obtained advantageously from a spacecraft placed in the proximity of the Sun-Earth Lagrange point L2. Maintaining the condition of continuous solar occultation by all parts of the atmospheric disk requires that the displacement of the spacecraft perpendicular to the Sun-Earth line remains less than 200 km. However, the gravitational force exerted by the Earth s moon must be negated by propulsion in order to meet this rather tight constraint. We provide an estimate of propulsive force needed to keep the spacecraft coincident with L2, as well as estimates of velocity increments needed to maintain various trajectories in the close vicinity of L2.

AmeriFlux CA-TP4 Ontario - Turkey Point 1939 Plantation White Pine

DOE Data Explorer

Arain, M. Altaf [McMaster University

2016-01-01

This is the AmeriFlux version of the carbon flux data for the site CA-TP4 Ontario - Turkey Point 1939 Plantation White Pine. Site Description - White pine plantation established in 1939 over sandy abandoned land

4Pi microscopy deconvolution with a variable point-spread function.

PubMed

Baddeley, David; Carl, Christian; Cremer, Christoph

2006-09-20

To remove the axial sidelobes from 4Pi images, deconvolution forms an integral part of 4Pi microscopy. As a result of its high axial resolution, the 4Pi point spread function (PSF) is particularly susceptible to imperfect optical conditions within the sample. This is typically observed as a shift in the position of the maxima under the PSF envelope. A significantly varying phase shift renders deconvolution procedures based on a spatially invariant PSF essentially useless. We present a technique for computing the forward transformation in the case of a varying phase at a computational expense of the same order of magnitude as that of the shift invariant case, a method for the estimation of PSF phase from an acquired image, and a deconvolution procedure built on these techniques.

Simulation of Shallow Water Jets with a Unified Element-based Continuous/Discontinuous Galerkin Model with Grid Flexibility on the Sphere

DTIC Science & Technology

2013-01-01

is the derivative of the N th-order Legendre polynomial . Given these definitions, the one-dimensional Lagrange polynomials hi(ξ) are hi(ξ) = − 1 N(N...2. Detail of one interface patch in the northern hemisphere. The high-order Legendre -Gauss-Lobatto (LGL) points are added to the linear grid by...smaller ones by a Lagrange polynomial of order nI . The number of quadrilateral elements and grid points of the final grid are then given by Np = 6(N

Perturbed Equations of Motion for Formation Flight Near the Sun-Earth L2 Point

NASA Technical Reports Server (NTRS)

Luquette, Richard; Segerman, A. M.; Zedd, M. F.

2005-01-01

NASA is planning missions to the vicinity of the Sun-Earth L(sub 2) point, some involving a distributed system of telescope spacecraft, configured in a plane about a hub. Several sets of differential equations are written for the formation flight of such telescopes relative to the hub, with varying levels of fidelity. Effects are cast as additive perturbations to the circular restricted three-body problem, expanded in terms of the system distanced, to an accuracy of 10-20 m. These include Earth's orbital eccentricity, lunar motion, solar radiation pressure, and small thrusting forces. Simulations validating the expanded differential equations are presented.

α-l-Arabinofuranosidase from Radish (Raphanus sativus L.) Seeds

PubMed Central

Hata, Keishi; Tanaka, Mika; Tsumuraya, Yoichi; Hashimoto, Yohichi

1992-01-01

An α-l-arabinofuranosidase has been purified 1043-fold from radish (Raphanus sativus L.) seeds. The purified enzyme was a homogeneous glycoprotein consisting of a single polypeptide with an apparent molecular weight of 64,000 and an isoelectric point value of 4.7, as evidenced by denaturing gel electrophoresis and reversed-phase or size-exclusion high-performance liquid chromatography and isoelectric focusing. The enzyme characteristically catalyzes the hydrolysis of p-nitrophenyl α-l-arabinofuranoside and p-nitrophenyl β-d-xylopyranoside in a constant ratio (3:1) of the initial velocities at pH 4.5, whereas the corresponding α-l-arabinopyranoside and β-d-xylofuranoside are unsusceptible. The following evidence was provided to support that a single enzyme with one catalytic site was responsible for the specificity: (a) high purity of the enzyme preparation, (b) an invariable ratio of the activities toward the two substrates throughout the purification steps, (c) a parallelism of the activities in activation with bovine serum albumin and in heat inactivation of the enzyme as well as in the inhibition with heavy metal ions and sugars such as Hg2+, Ag+, l-arabino-(1→4)-lactone, and d-xylose, and (d) results of the mixed substrate kinetic analysis using the two substrates. The enzyme was shown to split off α-l-arabinofuranosyl residues in sugar beet arabinan, soybean arabinan-4-galactan, and radish seed and leaf arabinogalactan proteins. Arabinose and xylose were released by the action of the enzyme on oat-spelt xylan. Synergistic action of α-l-arabinofuranosidase and β-d-galactosidase on radish seed arabinogalactan protein resulted in the extensive degradation of the carbohydrate moiety. Images Figure 2 PMID:16652973

Heteroclinic, Homoclinic Connections Between the Sun-Earth Triangular Points and Quasi-Satellite Orbits for Solar Observations

NASA Technical Reports Server (NTRS)

Llanos, Pedro J.; Hintz, Gerald R.; Lo, Martin W.; Miller, James K.

2013-01-01

Investigation of new orbit geometries exhibits a very attractive behavior for a spacecraft to monitor space weather coming from the Sun. Several orbit transfer mechanisms are analyzed as potential alternatives to monitor solar activity such as a sub-solar orbit or quasi-satellite orbit and short and long heteroclinic and homoclinic connections between the triangular points L(sub 4) and L(sub 5) and the collinear point L(sub 3) of the Circular Restricted Three-Body Problem (CRTBP) in the Sun-Earth system.

Validation of Accelerometer Cut-Points in Children With Cerebral Palsy Aged 4 to 5 Years.

PubMed

Keawutan, Piyapa; Bell, Kristie L; Oftedal, Stina; Davies, Peter S W; Boyd, Roslyn N

2016-01-01

To derive and validate triaxial accelerometer cut-points in children with cerebral palsy (CP) and compare these with previously established cut-points in children with typical development. Eighty-four children with CP aged 4 to 5 years wore the ActiGraph during a play-based gross motor function measure assessment that was video-taped for direct observation. Receiver operating characteristic and Bland-Altman plots were used for analyses. The ActiGraph had good classification accuracy in Gross Motor Function Classification System (GMFCS) levels III and V and fair classification accuracy in GMFCS levels I, II, and IV. These results support the use of the previously established cut-points for sedentary time of 820 counts per minute in children with CP aged 4 to 5 years across all functional abilities. The cut-point provides an objective measure of sedentary and active time in children with CP. The cut-point is applicable to group data but not for individual children.

Preparation and properties of Ge4Se96 glass

NASA Astrophysics Data System (ADS)

Xu, Junfeng; Sun, Guozhen; Liu, Zhenting; Wang, Yaling; Jian, Zengyun

2018-03-01

The chalcogenide glass Ge4Se96 was prepared by melt-quenched method. The characteristic temperatures (the strain point, annealing point, glass transition point, yielding point and soften point) were determined by thermal analysis. The result shows that the glass transition point is 52 °C; the average thermal expansion coefficient is ΔL/L0 = (0.0557∗T-1.7576)/1000. The specific heat of Ge4Se96 glass was measured by stepwise method. The difference in specific heat between amorphous structure and undercooled liquid was determined as ΔCp = 0.1812-5.20 × 10-4T-4.92 × 10-7T2 JK-1 g-1. Based on the result of ΔCp, the Gibbs energy and the entropy curves were calculated, and the Kauzmann temperature was determined as Tk = 233.5 K, which is between that for pure Se glass (216 K) and that for Ge7.4Se92.6 glass (250 K). It indicates that for Ge-Se glass with low Ge content, the Kauzmann temperature increases with increasing the content of Ge.

NSDC Policy Points. Volume 1, Number 4

ERIC Educational Resources Information Center

National Staff Development Council, 2009

2009-01-01

"NSDC Policy Points" is a newsletter published by the National Staff Development Council (NSDC). This issue of "NSDC Policy Points" discusses why collaborative learning is critical to professional development. Included in this newsletter is an article about team learning in Cedar Rapids, Iowa. [For Volume 1, Number 3 of…

Active and Passive Sensing from Geosynchronous and Libration Orbits

NASA Technical Reports Server (NTRS)

Schoeberl, Mark; Raymond, Carol; Hildebrand, Peter

2003-01-01

The development of the LEO (EOS) missions has led the way to new technologies and new science discoveries. However, LEO measurements alone cannot cost effectively produce high time resolution measurements needed to move the science to the next level. Both GEO and the Lagrange points, L1 and L2, provide vantage points that will allow higher time resolution measurements. GEO is currently being exploited by weather satellites, but the sensors currently operating at GEO do not provide the spatial or spectral resolution needed for atmospheric trace gas, ocean or land surface measurements. It is also may be possible to place active sensors in geostationary orbit. It seems clear, that the next era in earth observation and discovery will be opened by sensor systems operating beyond near earth orbit.

Thermodynamic Parameters of the Dissolution of 4-Hydroxy-L-Proline and L-Phenylalanine in Mixed Aqueous Solvents at 298 K

NASA Astrophysics Data System (ADS)

Smirnov, V. I.; Badelin, V. G.

2018-01-01

The enthalpies of solution of 4-hydroxy-L-proline and L-phenylalanine in binary mixed aqueous solvents containing acetonitrile (AN), 1,4-dioxane (1,4-DO), or acetone (AC) at mole fractions of 0 to 0.25 are determined at T = 298.15 K via isothermal calorimetry. The standard enthalpies of solution (Δsol H°) and transfer (Δtr H°) of 4-hydroxy-L-proline and L-phenylalanine from water to mixed aqueous solvents are calculated using the experimental calorimetric data, as are the enthalpy coefficients of paired interactions ( h xy ) between the molecules of the investigated amino acids and the organic solvents. The effects the mixed aqueous solvent composition and the structure of the organic solvent molecules have on the enthalpies of solution and transfer for the investigated amino acids are considered. The correlation between the enthalpy of solution of the amino acids and the electron-donating properties of the organic solvents in the mixed aqueous solvent systems is established.

Sagittal imbalance treated with L5 pedicle subtraction osteotomy with short lumbar fusion from L4 to sacrum using four screws into L4 for enhanced fixation two additional vertebral screws: a technical note.

PubMed

Wangdi, Kuenzang; Otsuki, Bungo; Fujibayashi, Shunsuke; Tanida, Shimei; Masamoto, Kazutaka; Matsuda, Shuichi

2018-02-07

To report on suggested technique with four screws in a single vertebra (two pedicle screws and two direct vertebral body screws) for enhanced fixation with just one level cranially to a pedicle subtraction osteotomy (PSO). A 60-year-old woman underwent L4/5 fusion surgery for degenerative spondylolisthesis. Two years later, she was unable to stand upright even for a short time because of lumbar kyphosis caused by subsidence of the fusion cage and of Baastrup syndrome in the upper lumbar spine [sagittal vertical axis (SVA) of 114 mm, pelvic incidence of 75°, and lumbar lordosis (LL) of 41°]. She underwent short-segment fusion from L4 to the sacrum with L5 pedicle subtraction osteotomy. We reinforced the construct with two vertebral screws at L4 in addition to the conventional L4 pedicle screws. After the surgery, her sagittal parameters were improved (SVA, 36 mm; LL, 54°). Two years after the corrective surgery, she maintained a low sagittal vertical axis though high residual pelvic tilt indicated that the patient was still compensating for residual sagittal misalignment. PSO surgery for sagittal imbalance usually requires a long fusion at least two levels above and below the osteotomy site to achieve adequate stability and better global alignment. However, longer fixation may decrease the patients' quality of life and cause a proximal junctional failure. Our novel technique may shorten the fixation area after osteotomy surgery. These slides can be retrieved under Electronic Supplementary Material.

Determination of L-AP4-bound human mGlu8 receptor amino terminal domain structure and the molecular basis for L-AP4’s group III mGlu receptor functional potency and selectivity

DOE Office of Scientific and Technical Information (OSTI.GOV)

Schkeryantz, Jeffery M.; Chen, Qi; Ho, Joseph D.

Here, L-2-Amino-4-phosphonobutyric acid (L-AP4) is a known potent and selective agonist for the Group III mGlu receptors. However, it does not show any selectivity among the individual group III mGlu subtypes. In order to understand the molecular basis for this group selectivity, we solved the first human mGlu8 amino terminal domain (ATD) crystal structures in complex with L-glu and L-AP4. In comparison with other published L-glu-bound mGlu ATD structures, we have observed L-glu binds in a significantly different manner in mGlu1. Furthermore, these new structures provided evidence that both the electronic and steric nature of the distal phosphate of L-AP4more » contribute to its exquisite Group III functional agonist potency and selectivity.« less

A Lunar L2-Farside Exploration and Science Mission Concept with the ORion Multi-Purpose Crew Vehicle and a Teleoperated Lander/Rover

NASA Technical Reports Server (NTRS)

Burns, Jack O.; Kring, David; Norris, Scott; Hopkins, Josh; Lazio, Joseph; Kasper, Justin

2012-01-01

A novel concept is presented in this paper for a human mission to the lunar L2 (Lagrange) point that would be a proving ground for future exploration missions to deep space while also overseeing scientifically important investigations. In an L2 halo orbit above the lunar farside, the astronauts would travel 15% farther from Earth than did the Apollo astronauts and spend almost three times longer in deep space. Such missions would validate the Orion MPCV's life support systems, would demonstrate the high-speed re-entry capability needed for return from deep space, and would measure astronauts' radiation dose from cosmic rays and solar flares to verify that Orion would provide sufficient protection, as it is designed to do. On this proposed mission, the astronauts would teleoperate landers and rovers on the unexplored lunar farside, which would obtain samples from the geologically interesting farside and deploy a low radio frequency telescope. Sampling the South Pole-Aitkin basin (one of the oldest impact basins in the solar system) is a key science objective of the 2011 Planetary Science Decadal Survey. Observations of the Universe's first stars/galaxies at low radio frequencies are a priority of the 2010 Astronomy & Astrophysics Decadal Survey. Such telerobotic oversight would also demonstrate capability for human and robotic cooperation on future, more complex deep space missions.

76 FR 26771 - Florida Power & Light Company; Turkey Point, Units 3 and 4; Notice of Consideration of Issuance...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-05-09

... Company; Turkey Point, Units 3 and 4; Notice of Consideration of Issuance of Amendment to Facility... issued to Florida Power & Light Co. (the licensee) for operation of the Turkey Point Nuclear Generating... licensed core power level for Turkey Point, Units 3 and 4, from 2300 megawatts thermal (MWt) to 2644 MWt...

Early development and the point of no return in pikeperch ( Sander lucioperca L.) larvae

NASA Astrophysics Data System (ADS)

Xu, Zhengchao; Li, Caijuan; Ling, Qufei; Gaughan, Sarah; Wang, Guocheng; Han, Xiaofei

2017-11-01

The objectives of the present study were to evaluate the yolk-sac and oil globule absorption and point of no return (PNR) of pikeperch ( Sander lucioperca L.) larvae. Artificial propagation of pikeperch was performed at (15±2)°C. Yolk-sac absorption, oil globule absorption, larval growth and the first initial feeding rate were observed to analyze the early growth and to determine the PNR of pikeperch larvae. The total length of newly hatched (0 day after hatching, DAH) pikeperch larvae was (4.25±0.22) mm and the volume of the yolk-sac and the oil globule was (0.30±0.12) mm3 and (5.14±2.28) 10-2 mm3 respectively. The yolk-sac and the oil globule were exhausted at 11 DAH and 14 DAH, respectively. Pikeperch larvae began feeding at 8 DAH with an initial feeding rate about 10.0%. From 9 to 14 DAH, the initial feeding rate increased rapidly, and reached its highest (about 82.7%) at 14 DAH. It declined thereafter, 48.9% at 15 DAH and 35.6% at 16 DAH, thus the pikeperch larvae reached PNR by 15-16 DAH. The appropriate first feeding time for the pikeperch larvae is 11-12 DAH, when the initial feeding rate is higher than half of the maximum initial feeding rate.

Lagrange thermodynamic potential and intrinsic variables for He-3 He-4 dilute solutions

NASA Technical Reports Server (NTRS)

Jackson, H. W.

1983-01-01

For a two-fluid model of dilute solutions of He-3 in liquid He-4, a thermodynamic potential is constructed that provides a Lagrangian for deriving equations of motion by a variational procedure. This Lagrangian is defined for uniform velocity fields as a (negative) Legendre transform of total internal energy, and its primary independent variables, together with their thermodynamic conjugates, are identified. Here, similarities between relations in classical physics and quantum statistical mechanics serve as a guide for developing an alternate expression for this function that reveals its character as the difference between apparent kinetic energy and intrinsic internal energy. When the He-3 concentration in the mixtures tends to zero, this expression reduces to Zilsel's formula for the Lagrangian for pure liquid He-4. An investigation of properties of the intrinsic internal energy leads to the introduction of intrinsic chemical potentials along with other intrinsic variables for the mixtures. Explicit formulas for these variables are derived for a noninteracting elementary excitation model of the fluid. Using these formulas and others also derived from quantum statistical mechanics, another equivalent expression for the Lagrangian is generated.

78 FR 45592 - DeltaPoint Capital IV, LP;

Federal Register 2010, 2011, 2012, 2013, 2014

2013-07-29

... Regulations (13 CFR 107.730). DeltaPoint Capital IV, L.P. provided financing to BioMaxx, Inc., 1 Fishers Road... York), L.P., an Associate of DeltaPoint Capital IV, L.P., owns more than ten percent of BioMaxx, Inc...

On the error propagation of semi-Lagrange and Fourier methods for advection problems☆

PubMed Central

Einkemmer, Lukas; Ostermann, Alexander

2015-01-01

In this paper we study the error propagation of numerical schemes for the advection equation in the case where high precision is desired. The numerical methods considered are based on the fast Fourier transform, polynomial interpolation (semi-Lagrangian methods using a Lagrange or spline interpolation), and a discontinuous Galerkin semi-Lagrangian approach (which is conservative and has to store more than a single value per cell). We demonstrate, by carrying out numerical experiments, that the worst case error estimates given in the literature provide a good explanation for the error propagation of the interpolation-based semi-Lagrangian methods. For the discontinuous Galerkin semi-Lagrangian method, however, we find that the characteristic property of semi-Lagrangian error estimates (namely the fact that the error increases proportionally to the number of time steps) is not observed. We provide an explanation for this behavior and conduct numerical simulations that corroborate the different qualitative features of the error in the two respective types of semi-Lagrangian methods. The method based on the fast Fourier transform is exact but, due to round-off errors, susceptible to a linear increase of the error in the number of time steps. We show how to modify the Cooley–Tukey algorithm in order to obtain an error growth that is proportional to the square root of the number of time steps. Finally, we show, for a simple model, that our conclusions hold true if the advection solver is used as part of a splitting scheme. PMID:25844018

Enhancement of stability of L-tryptophan dehydrogenase from Nostoc punctiforme ATCC29133 and its application to L-tryptophan assay.

PubMed

Matsui, Daisuke; Okazaki, Seiji; Matsuda, Motoki; Asano, Yasuhisa

2015-02-20

Microbial NAD(+)-dependent L-tryptophan dehydrogenase (TrpDH, EC1.4.1.19), which catalyzes the reversible oxidative deamination and the reductive amination between L-tryptophan and indole-3-pyruvic acid, was found in the scytonemin biosynthetic pathway of Nostoc punctiforme ATCC29133. The TrpDH exhibited high specificity toward L-tryptophan, but its instability was a drawback for L-tryptophan determination. The mutant enzyme TrpDH L59F/D168G/A234D/I296N with thermal stability was obtained by screening of Escherichia coli transformants harboring various mutant genes, which were generated by error-prone PCR using complementation in an L-tryptophan auxotroph of E. coli. The specific activity and stability of this mutant enzyme were higher than those of the wild type enzyme. We also revealed here that in these four mutation points, the two amino acid residues Asp168 and Ile296 contributed to increase the enzyme stability, and the Leu59, Ala234 residues to increase its specific activity. Growth of the strain harboring the gene of above 4 point mutated enzyme was accelerated by the enhanced performance. In the present study, we demonstrated that TrpDH L59F/D168G/A234D/I296N was available for determination of L-tryptophan in human plasma. Copyright © 2015 Elsevier B.V. All rights reserved.

Pairwise Interaction Extended Point-Particle (PIEP) model for multiphase jets and sedimenting particles

NASA Astrophysics Data System (ADS)

Liu, Kai; Balachandar, S.

2017-11-01

We perform a series of Euler-Lagrange direct numerical simulations (DNS) for multiphase jets and sedimenting particles. The forces the flow exerts on the particles in these two-way coupled simulations are computed using the Basset-Bousinesq-Oseen (BBO) equations. These forces do not explicitly account for particle-particle interactions, even though such pairwise interactions induced by the perturbations from neighboring particles may be important especially when the particle volume fraction is high. Such effects have been largely unaddressed in the literature. Here, we implement the Pairwise Interaction Extended Point-Particle (PIEP) model to simulate the effect of neighboring particle pairs. A simple collision model is also applied to avoid unphysical overlapping of solid spherical particles. The simulation results indicate that the PIEP model provides a more elaborative and complicated movement of the dispersed phase (droplets and particles). Office of Naval Research (ONR) Multidisciplinary University Research Initiative (MURI) project N00014-16-1-2617.

Effects of Nigella sativa L. and Urtica dioica L. on selected mineral status and hematological values in CCl4-treated rats.

PubMed

Meral, Ismail; Kanter, Mehmet

2003-01-01

This study was designed to investigate the effects of Nigella sativa L. (NS), known as black seed, or/and Urtica dioica L. (UD), known as stinging nettle root, treatments on serum Na, K, Cl, and Ca levels and some hematological values of CCl4-treated rats. Sixty healthy male Sprague-Dawley rats, weighing 250-300 g, were randomly allotted into 1 of 4 experimental groups: A (CCl4-only treated), B (CCl4+UD treated), C (CCl4+NS treated), and D (CCl4+UD+NS treated), each containing 15 animals. All groups received CCl4 (0.8 mL/kg of body weight, subcutaneously, twice a week for 90 d starting d 1). In addition, B, C, and D groups also received the daily ip injection of 0.2 mL/kg NS and/or 2 mL/kg UD oils for 45 d starting d 46. Group A, on the other hand, received only 2 mL/kg normal saline solution for 45 d starting d 46. Blood samples for the biochemical analysis were taken by cardiac puncture from five randomly chosen rats in each treatment group at the beginning, d 45, and d 90 of the experiment. The CCl4 treatment for 45 d significantly (p<0.05) increased the serum K and Ca and decreased (p<0.05) the red blood cell count (RBC), white blood cell count (WBC), packed cell volume (PCV), and Hb levels without changing (p>0.05) the serum Na and Cl levels. NS or UD treatments (alone or combination) for 45 d starting d 46 significantly (p<0.05) decreased the elevated serum K and Ca levels and also increased (p<0.05) the reduced RBC, WBC, PCV, and Hb levels. It is concluded that NS and/or UD treatments might ameliorate the CCl4-induced disturbances of anemia, some minerals, and body's defense mechanism in CCl4-treated rats.

’Point of Injury’ Sampling Technology for Battlefield Molecular Diagnostics

DTIC Science & Technology

2012-03-17

Injury" Sampling Technology for Battlefield Molecular Diagnostics March 17,2012 Sponsored by Defense Advanced Research Projects Agency (DOD) Defense...Contract: April 25, 2011 Short Title of Work: "Point of Injury" Sampling Technology for Battlefield Molecular Diagnostics " Contract Expiration Date...SBIR PHASE I OPTION REPORT: Point of Injury, Sampling Technology for Battlefield Molecular Diagnostics . W31P4Q-1 l-C-0222 (UNCLASSIFIED) P.I

Numerical and In Vitro Experimental Investigation of the Hemolytic Performance at the Off-Design Point of an Axial Ventricular Assist Pump.

PubMed

Liu, Guang-Mao; Jin, Dong-Hai; Jiang, Xi-Hang; Zhou, Jian-Ye; Zhang, Yan; Chen, Hai-Bo; Hu, Sheng-Shou; Gui, Xing-Min

The ventricular assist pumps do not always function at the design point; instead, these pumps may operate at unfavorable off-design points. For example, the axial ventricular assist pump FW-2, in which the design point is 5 L/min flow rate against 100 mm Hg pressure increase at 8,000 rpm, sometimes works at off-design flow rates of 1 to 4 L/min. The hemolytic performance of the FW-2 at both the design point and at off-design points was estimated numerically and tested in vitro. Flow characteristics in the pump were numerically simulated and analyzed with special attention paid to the scalar sheer stress and exposure time. An in vitro hemolysis test was conducted to verify the numerical results. The simulation results showed that the scalar shear stress in the rotor region at the 1 L/min off-design point was 70% greater than at the 5 L/min design point. The hemolysis index at the 1 L/min off-design point was 3.6 times greater than at the 5 L/min design point. The in vitro results showed that the normalized index of hemolysis increased from 0.017 g/100 L at the 5 L/min design point to 0.162 g/100 L at the 1 L/min off-design point. The hemolysis comparison between the different blood pump flow rates will be helpful for future pump design point selection and will guide the usage of ventricular assist pumps. The hemolytic performance of the blood pump at the working point in the clinic should receive more focus.

26 CFR 1.167(l)-4 - Public utility property; election to use asset depreciation range system.

Code of Federal Regulations, 2010 CFR

2010-04-01

... depreciation range system. 1.167(l)-4 Section 1.167(l)-4 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT... Individuals and Corporations § 1.167(l)-4 Public utility property; election to use asset depreciation range system. (a) Application of section 167(l) to certain property subject to asset depreciation range system...

EphrinA4 plays a critical role in α4 and αL mediated survival of human CLL cells during extravasation

PubMed Central

Trinidad, Eva M.; García, Dolores; Soler, Gloria; Ortuño, Francisco J.; Zapata, Agustín G.; Alonso, Luis M.

2016-01-01

A role of endothelial cells in the survival of CLL cells during extravasation is presently unknown. Herein we show that CLL cells but not normal B cells can receive apoptotic signals through physical contact with TNF-α activated endothelium impairing survival in transendothelial migration (TEM) assays. In addition, the CLL cells of patients having lymphadenopathy (LApos) show a survival advantage during TEM that can be linked to increased expression of α4 and αL integrin chains. Within this context, ephrinA4 expressed on the surface of CLL cells sequestrates integrins and inactivates them resulting in reduced adhesion and inhibition of apoptotic/survival signals through them. In agreement, ephrinA4 silencing resulted in increased survival of CLL cells of LApos patients but not LA neg patients. Similarly was observed when a soluble ephrinA4 isoform was added to TEM assays strongly suggesting that accumulation of this isoform in the serum of LApos patients could contribute to CLL cells dissemination and survival in vivo. In supporting, CLL lymphadenopathies showed a preferential accumulation of apoptotic CLL cells around high endothelial venules lacking ephrinA4. Moreover, soluble ephrinA4 isolated from sera of patients increased the number and viability of CLL cells recovered from the lymph nodes of adoptively transferred mice. Finally, we present evidence suggesting that soluble ephrinA4 mediated survival during TEM could enhance a transcellular TEM route of the CLL cells. Together these findings point to an important role of ephrinA4 in the nodal dissemination of CLL cells governing extravasation and survival. PMID:27374180

4,4'-Dichlorodiphenyltrichloroethane (DDT) and 4,4'-dichlorodiphenyldichloroethylene (DDE) promote adipogenesis in 3T3-L1 adipocyte cell culture.

PubMed

Kim, Jonggun; Sun, Quancai; Yue, Yiren; Yoon, Kyong Sup; Whang, Kwang-Youn; Marshall Clark, J; Park, Yeonhwa

2016-07-01

4,4'-Dichlorodiphenyltrichloroethane (DDT), a chlorinated hydrocarbon insecticide, was extensively used in the 1940s and 1950s. DDT is mainly metabolically converted into 4,4'-dichlorodiphenyldichloroethylene (DDE). Even though most countries banned DDT in the 1970s, due to the highly lipophilic nature and very stable characteristics, DDT and its metabolites are present ubiquitously in the environment, including food. Recently, there are publications on relationships between exposure to insecticides, including DDT and DDE, and weight gain and altered glucose homeostasis. However, there are limited reports regarding DDT or DDE and adipogenesis, thus we investigated effects of DDT and DDE on adipogenesis using 3T3-L1 adipocytes. Treatment of DDT or DDE resulted in increased lipid accumulation accompanied by increased expression of CCAAT/enhancer-binding protein α (C/EBPα), peroxisome-proliferator activated receptor-γ (PPARγ), fatty acid synthase (FAS), acetyl-CoA carboxylase (ACC), adipose triglyceride lipase, and leptin. Moreover, treatment of DDT or DDE increased protein levels of C/EBPα, PPARγ, AMP-activated protein kinase-α (AMPKα), and ACC, while significant decrease of phosphorylated forms of AMPKα and ACC were observed. These finding suggest that increased lipid accumulation caused by DDT and DDE may mediate AMPKα pathway in 3T3-L1 adipocytes. Copyright © 2016 Elsevier B.V. All rights reserved.

Crystal Structure of an L-Carnitine Complex with Pyrogallol[4]arene

NASA Astrophysics Data System (ADS)

Fujisawa, I.; Takeuchi, D.; Kitamura, Y.; Okamoto, R.; Aoki, K.

2012-03-01

L-Carnitine is essential for the transport of long-chain fatty acids from cytosol into mitochondria for generating metabolic energy. The survey of crystal structures of carnitine-containing proteins in the Protein Data Bank reveals that carnitine can take several conformations with the quarternary trimethylammonium terminal being always bound to aromatic residues through cation-π interactions in acyltransferases or carnitine-binding proteins. In order to demonstrate the importance of cation-π interaction as a carnitine recognition mechanism in the artificial receptor-ligand system that mimics the carnitine-binding sites, we have determined the crystal structure of a complex formed between L-carnitine and pyrogallol[4]arene (pyrogallol cyclic tetramer: PCT) as a carnitine receptor, 2PCT·2(L-carnitine)·4EtOH. There form two crystallographically independent monomeric [PCT·L-carnitine] substructures, which further form an obliquely arranged capsule-like dimeric [PCT·L-carnitine]2 structure through a pair of O-H (PCT)···O (L-carnitine) hydrogen bonds. This is the first report of PCT complex with chiral molecules. In each of the two monomeric [PCT·L-carnitine] substructures, the L-carnitine molecule takes the elongated form with an intramolecular hydrogen bond between the hydroxyl group and the carboxylate oxygen, and the cationic trimethylammonium moiety is incorporated into the cavity of the bowl-shaped PCT molecule through cation-π interactions. These features are similar to those at the D-carnitine-binding site in the crystal structure of the glycine betaine/carnitine/choline-binding protein complex.

Evaluation of quality of life in adolescent idiopathic scoliosis with different distal fusion level: a comparison of L3 versus L4.

PubMed

Ding, Ran; Liang, Jinqian; Qiu, Guixing; Shen, Jianxiong; Li, Zheng

2014-07-01

Retrospective case-control study. The aim of this study was to assess patients' quality of life with different fusion levels in posterior pedicle screw correction of idiopathic scoliosis. No previous study has demonstrated differences in health-related quality of life (HRQoL) between patients fused to L3 versus L4. A retrospective study was conducted on 30 scoliotic patients fused to L3 using complete pedicle screw instruments. Thirty age-matched and sex-matched scoliotic patients fused to L4 were in the control group. Radiologic parameters were assessed before surgery and at latest follow-up between the 2 groups. These 2 groups were compared for the Scoliosis Research Society 22 questionnaire (SRS-22), Oswestry Disability Index (ODI), visual analogue scale (VAS), and Short Form-36 (SF-36) questionnaires, which were administered preoperatively and at final follow-up. There were no significant differences in sex, age, follow-up duration, the distribution of curve patterns, the postoperative residual Cobb angle of the main curve, complications, or surgical method between the L3 and L4 groups (P>0.05). Preoperative scores were statistically similar in the L3 and L4 groups for all domains of all questionnaires. There was no difference between the L3 and L4 groups for ODI (P=0.527) and VAS (P=0.518). There were no significant differences in the scores on function/activity, self-image/appearance, pain, mental health, or satisfaction with treatment domains between the 2 groups. No significant differences between the 2 groups were found at the final follow-up in the SF-36 subscales/domain scores. This study attempted to elucidate the correlation between the length of fusion and functional outcome; however, it could not identify any difference between different fusion levels. On the basis of short-term results, there were no significant differences in the questionnaire scores between the 2 groups.

Deoxyfluoroketohexoses: 4-deoxy-4-fluoro-D-sorbose and -tagatose and 5-deoxy-5-fluoro-L-sorbose.

PubMed

Rao, G V; Que, L; Hall, L D; Fondy, T P

1975-04-01

4-Deoxy-4-fluoro-alpha-D-sorbose (6) was prepared in crystalline form by the action of potassium hydrogen fluoride on 3,4-anhydro-1,2-O-isopropylidene-beta-D-psicopyranose (3) followed by deacetonation. Under identical conditions, 3,4-anhydro-1,2-O-isopropylidene-beta-D-tagatopyranose (7) underwent epoxide migration to give 4,5-anhydro-1,2-O-isopropylidene-beta-D-fructopyranose (12), which after deacetonation yielded 4-deoxy-4-fluoro-D-tagatose (15) and 5-deoxy-5-fluoro-alpha-L-sorbopyranose (16), the latter as the crystalline, free sugar. The action of glycol-cleavage reagents on the isopropylidene acetals of the deoxyfluoro sugars was consistent with the assigned structures. The structures were established by 13-C n.m.r. studies of the free deoxyfluoro sugars 6 and 16 and of the isopropylidene acetal 13, and by 1-H n.m.r. studies on the acetylated isopropylidene acetals 5 diacetate, 13 diacetate, and 14 diacetate. 5-Deoxy-5-fluoro-L-sorbose (16) was biologically active, producing in mice effects characteristic of deoxyfluorotrioses and of fluoroacetate. 4-Deoxy-4-fluoro-D-tagatose (15) and 4-deoxy-4-fluoro-D-sorbose (6) produced no apparent effects in mice up to a dose of 500mg/kg. The implications of these findings with respect to transport, phosphorylation, and the action of aldolase on ketohexoses are discussed.

Point mutations in the tumor suppressor Smad4/DPC4 enhance its phosphorylation by GSK3 and reversibly inactivate TGF-β signaling

PubMed Central

Demagny, Hadrien; De Robertis, Edward M

2016-01-01

The tumor suppressor Smad4/DPC4 is an essential transcription factor in the TGF-β pathway and is frequently mutated or deleted in prostate, colorectal, and pancreatic carcinomas. We recently discovered that Smad4 activity and stability are regulated by the FGF/EGF and Wnt signaling pathways through a series of MAPK and GSK3 phosphorylation sites located in its linker region. In the present study, we report that loss-of-function associated with 2 point mutations commonly found in colorectal and pancreatic cancers results from enhanced Smad4 phosphorylation by GSK3, generating a phosphodegron that leads to subsequent β-TrCP–mediated polyubiquitination and proteasomal degradation. Using chemical GSK3 inhibitors, we show that Smad4 point mutant proteins can be stabilized and TGF-β signaling restored in cancer cells harboring such mutations. PMID:27308538

4. James L. Dillon and Company, Inc., photographer January, 1967 ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

4. James L. Dillon and Company, Inc., photographer January, 1967 INTERESTING OVAL STAIRWELL, LOOKING STRAIGHT UP FROM SECOND FLOOR - 626 South Front Street (House), Philadelphia, Philadelphia County, PA

A Novel Pathogenesis-Related Class 10 Protein Gly m 4l, Increases Resistance upon Phytophthora sojae Infection in Soybean (Glycine max [L.] Merr.)

PubMed Central

Fan, Sujie; Jiang, Liangyu; Wu, Junjiang; Dong, Lidong; Cheng, Qun; Xu, Pengfei; Zhang, Shuzhen

2015-01-01

Phytophthora root and stem rot of soybean, caused by Phytophthora sojae (P. sojae), is a destructive disease in many soybean planting regions worldwide. In a previous study, an expressed sequence tag (EST) homolog of the major allergen Pru ar 1 in apricot (Prunus armeniaca) was identified up-regulated in the highly resistant soybean ‘Suinong 10’ infected with P. sojae. Here, the full length of the EST was isolated using rapid amplification of cDNA ends (RACE). It showed the highest homolgy of 53.46% with Gly m 4 after comparison with the eight soybean allergen families reported and was named Gly m 4-like (Gly m 4l, GenBank accession no. HQ913577.1). The cDNA full length of Gly m 4l was 707 bp containing a 474 bp open reading frame encoding a polypeptide of 157 amino acids. Sequence analysis suggests that Gly m 4l contains a conserved ‘P-loop’ (phosphate-binding loop) motif at residues 47–55 aa and a Bet v 1 domain at residues 87–120 aa. The transcript abundance of Gly m 4l was significantly induced by P. sojae, salicylic acid (SA), NaCl, and also responded to methyl jasmonic acid (MeJA) and ethylene (ET). The recombinant Gly m 4l protein showed RNase activity and displayed directly antimicrobial activity that inhibited hyphal growth and reduced zoospore release in P. sojae. Further analyses showed that the RNase activity of the recombinant protein to degrading tRNA was significantly affected in the presence of zeatin. Over-expression of Gly m 4l in susceptible ‘Dongnong 50’ soybean showed enhanced resistance to P. sojae. These results indicated that Gly m 4l protein played an important role in the defense of soybean against P. sojae infection. PMID:26474489

Newly occurred L4 spondylolysis in the lumbar spine with pre-existence L5 spondylolysis among sports players: case reports and biomechanical analysis.

PubMed

Sairyo, Koichi; Sakai, Toshinori; Yasui, Natsuo; Kiapour, Ali; Biyani, Ashok; Ebraheim, Nabil; Goel, Vijay K

2009-10-01

Case series and a biomechanical study using a finite element (FE) analysis. To report three cases with multi-level spondylolysis and to understand the mechanism biomechanically. Multi-level spondylolysis is a very rare condition. There have been few reports in the literature on multi-level spondylolysis among sports players. We reviewed three cases of the condition, clinically. These patients were very active young sports players and had newly developed fresh L4 spondylolysis and pre-existing L5 terminal stage spondylolysis. Thus, we assumed that L5 spondylolysis may have increased the pars stress at the cranial adjacent levels, leading to newly developed spondylolysis at these levels. Biomechanically, we investigated pars stress at L4 with or without spondylolysis at L5 using the finite element technique. L4 pars stress decreased in the presence of L5 spondylolysis, which does not support our first hypothesis. It seems that multi-level spondylolysis may occur due to genetic and not biomechanical reasons.

Enhanced synthesis of L-threo-3,4-dihydroxyphenylserine by high-density whole-cell biocatalyst of recombinant L-threonine aldolase from Streptomyces avelmitilis.

PubMed

Baik, Sang-Ho; Yoshioka, Hideki

2009-03-01

L-threo-3,4-Dihydroxyphenylserine (DOPS) is a chiral unnatural beta-hydroxy amino acid used for the treatment of Parkinson disease. We developed a continuous bioconversion system for DOPS production that uses whole-cell biocatalyst of recombinant Escherichia coli expressing L-threonine aldolase (L-TA) genes cloned from Streptomyces avelmitilis MA-4680. Maximum conversion rates were observed at 2 M glycine, 145 mM 3,4-dihydroxybenzaldehyde, 0.75% Triton-X, 5 g E. coli cells/l, pH 6.5 and 10 degrees C. In the optimized condition, overall productivity was 8 g/l, which represents 40 times the synthesis yield possible with no optimization of conditions.

4. Historic American Buildings Survey Allen L. Hubbard, Photographer December ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

4. Historic American Buildings Survey Allen L. Hubbard, Photographer December 1, 1936 FIREPLACE WALL (northwest room 1st floor) - Fort Western, Main Building, Bowman Street, Augusta, Kennebec County, ME

Ubiquitin ligase Nedd4L targets activated Smad2/3 to limit TGF-beta signaling.

PubMed

Gao, Sheng; Alarcón, Claudio; Sapkota, Gopal; Rahman, Sadia; Chen, Pan-Yu; Goerner, Nina; Macias, Maria J; Erdjument-Bromage, Hediye; Tempst, Paul; Massagué, Joan

2009-11-13

TGF-beta induces phosphorylation of the transcription factors Smad2 and Smad3 at the C terminus as well as at an interdomain linker region. TGF-beta-induced linker phosphorylation marks the activated Smad proteins for proteasome-mediated destruction. Here, we identify Nedd4L as the ubiquitin ligase responsible for this step. Through its WW domain, Nedd4L specifically recognizes a TGF-beta-induced phosphoThr-ProTyr motif in the linker region, resulting in Smad2/3 polyubiquitination and degradation. Nedd4L is not interchangeable with Smurf1, a ubiquitin ligase that targets BMP-activated, linker-phosphorylated Smad1. Nedd4L limits the half-life of TGF-beta-activated Smads and restricts the amplitude and duration of TGF-beta gene responses, and in mouse embryonic stem cells, it limits the induction of mesoendodermal fates by Smad2/3-activating factors. Hierarchical regulation is provided by SGK1, which phosphorylates Nedd4L to prevent binding of Smad2/3. Previously identified as a regulator of renal sodium channels, Nedd4L is shown here to play a broader role as a general modulator of Smad turnover during TGF-beta signal transduction.

Y4lO of Rhizobium sp. strain NGR234 is a symbiotic determinant required for symbiosome differentiation.

PubMed

Yang, Feng-Juan; Cheng, Li-Li; Zhang, Ling; Dai, Wei-Jun; Liu, Zhe; Yao, Nan; Xie, Zhi-Ping; Staehelin, Christian

2009-02-01

Type 3 (T3) effector proteins, secreted by nitrogen-fixing rhizobia with a bacterial T3 secretion system, affect the nodulation of certain host legumes. The open reading frame y4lO of Rhizobium sp. strain NGR234 encodes a protein with sequence similarities to T3 effectors from pathogenic bacteria (the YopJ effector family). Transcription studies showed that the promoter activity of y4lO depended on the transcriptional activator TtsI. Recombinant Y4lO protein expressed in Escherichia coli did not acetylate two representative mitogen-activated protein kinase kinases (human MKK6 and MKK1 from Medicago truncatula), indicating that YopJ-like proteins differ with respect to their substrate specificities. The y4lO gene was mutated in NGR234 (strain NGROmegay4lO) and in NGR Omega nopL, a mutant that does not produce the T3 effector NopL (strain NGR Omega nopLOmegay4lO). When used as inoculants, the symbiotic properties of the mutants differed. Tephrosia vogelii, Phaseolus vulgaris cv. Yudou No. 1, and Vigna unguiculata cv. Sui Qing Dou Jiao formed pink effective nodules with NGR234 and NGR Omega nopL Omega y4lO. Nodules induced by NGR Omega y4lO were first pink but rapidly turned greenish (ineffective nodules), indicating premature senescence. An ultrastructural analysis of the nodules induced by NGR Omega y4lO revealed abnormal formation of enlarged infection droplets in ineffective nodules, whereas symbiosomes harboring a single bacteroid were frequently observed in effective nodules induced by NGR234 or NGR Omega nopL Omega y4lO. It is concluded that Y4lO is a symbiotic determinant involved in the differentiation of symbiosomes. Y4lO mitigated senescence-inducing effects caused by the T3 effector NopL, suggesting synergistic effects for Y4lO and NopL in nitrogen-fixing nodules.

The SMAP Level 4 Surface and Root-zone Soil Moisture (L4_SM) Product

NASA Technical Reports Server (NTRS)

Reichle, Rolf; Crow, Wade; Koster, Randal; Kimball, John

2010-01-01

The Soil Moisture Active and Passive (SMAP) mission is being developed by NASA for launch in 2013 as one of four first-tier missions recommended by the U.S. National Research Council Committee on Earth Science and Applications from Space in 2007. The primary science objectives of SMAP are to enhance understanding of land surface controls on the water, energy and carbon cycles, and to determine their linkages. Moreover, the high resolution soil moisture mapping provided by SMAP has practical applications in weather and seasonal climate prediction, agriculture, human health, drought and flood decision support. In this paper we describe the assimilation of SMAP observations for the generation of the planned SMAP Level 4 Surface and Root-zone Soil Moisture (L4_SM) product. The SMAP mission makes simultaneous active (radar) and passive (radiometer) measurements in the 1.26-1.43 GHz range (L-band) from a sun-synchronous low-earth orbit. Measurements will be obtained across a 1000 km wide swath using conical scanning at a constant incidence angle (40 deg). The radar resolution varies from 1-3 km over the outer 70% of the swath to about 30 km near the center of the swath. The radiometer resolution is 40 km across the entire swath. The radiometer measurements will allow high-accuracy but coarse resolution (40 km) measurements. The radar measurements will add significantly higher resolution information. The radar is however very sensitive to surface roughness and vegetation structure. The combination of the two measurements allows optimal blending of the advantages of each instrument. SMAP directly observes only surface soil moisture (in the top 5 cm of the soil column). Several of the key applications targeted by SMAP, however, require knowledge of root zone soil moisture (approximately top 1 m of the soil column), which is not directly measured by SMAP. The foremost objective of the SMAP L4_SM product is to fill this gap and provide estimates of root zone soil moisture

The Next Generation Space Telescope

NASA Technical Reports Server (NTRS)

Mather, John C.; Seery, Bernard (Technical Monitor)

2001-01-01

The Next Generation Space Telescope NGST is an 6-7 m class radiatively cooled telescope, planned for launch to the Lagrange point L2 in 2009, to be built by a partnership of NASA, ESA, and CSA. The NGST science program calls for three core instruments: 1) Near IR camera, 0.6 - 5 micrometer; 2) Near IR multiobject spectrometer, 1 - 5 micrometer, and 3) Mid IR camera and spectrometer, 5 - 28 micrometers. I will report on the scientific goals, project status, and the recent reduction in aperture from the target of 8 m.

L -subshell ionization of Ce, Nd, and Lu by 4-10-MeV C ions

NASA Astrophysics Data System (ADS)

Lapicki, G.; Mandal, A. C.; Santra, S.; Mitra, D.; Sarkar, M.; Bhattacharya, D.; Sen, P.; Sarkadi, L.; Trautmann, D.

2005-08-01

Ll,Lα,Lβ,Lγ,Lγ1+5,Lγ2+3,Lγ4+4' x-ray production cross sections of Ce58 , Nd60 and Lu71 induced by 4-, 6-, 8-, and 10-MeV carbon ions were measured. For Lu, Lγ2+3 is separated from Lγ2+3+6 after revision of the technique of Datz so that Lγ1+5 was used instead of Lγ1 , the Lγ4+4'/Lγ1+5 ratio was corrected for multiple ionization, and uncertainties in Lγ4+4' were incorporated in the fitting process. L -subshell ionization cross sections were extracted as a weighted average from two combinations of these cross sections, {Lα,Lγ1+5,Lγ2+3} and {Lα,Lγ1+5,Lγ} . It is shown that, to within a few percent, the first of these two combinations results in the identical cross sections as this weighted average. Within 10%, permutations of different sets of single-hole atomic parameters yielded the same ionization cross sections. These cross sections are typically within 15% and at most 35% of the cross sections obtained with atomic parameters that were altered in two different ways for multiple ionization. Extracted subshell and total L -shell ionization cross sections as well as Ce and Nd data of Braziewicz are compared with the ECPSSR theory of Brandt and Lapicki that accounts for the energy-loss (E), Coulomb-deflection (C), perturbed-stationary-state (PSS) and relativistic (R) effects. These measurements are also compared with the ECPSSR theory after its corrections—in a separated and united atom (USA) treatment, and for the intrashell (IS) transitions with the factors of Sarkadi and Mukoyama normalized to match L -shell cross section with the sum of L -subshell cross sections—as well as with the similarly improved semiclassical approximation of Trautmann. For Ce and Nd, the agreement of the extracted ionization cross sections with these theories is poor for L1 and good for L2 , L3 , and total L shell ionization. For the L2 subshell, this agreement is better for Ce and Nd than for Lu. The ECPSSR theory corrected for the USA and IS effects is surprisingly

MULTI-WAVELENGTH OBSERVATIONS OF COMET C/2011 L4 (PAN-STARRS)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Yang, Bin; Keane, Jacqueline; Meech, Karen

2014-04-01

The dynamically new comet C/2011 L4 (Pan-STARRS) is one of the brightest comets observed since the great comet C/1995 O1 (Hale-Bopp). Here, we present our multi-wavelength observations of C/2011 L4 during its in-bound passage to the inner solar system. A strong absorption band of water ice at 2.0 μm was detected in the near-infrared spectra, obtained with the 8 m Gemini-North and 3 m Infrared Telescope Facility Telescopes. The companion 1.5 μm band of water ice, however, was not observed. Spectral modeling shows that the absence of the 1.5 μm feature can be explained by the presence of sub-micron-sized fine ice grains.more » No gas lines (i.e., CN, HCN, or CO) were observed pre-perihelion in either the optical or the submillimeter. We derived 3σ upper limits for the CN and CO production rates. The comet exhibited a very strong continuum in the optical and its slope seemed to become redder as the comet approached the Sun. Our observations suggest that C/2011 L4 is an unusually dust-rich comet with a dust-to-gas mass ratio >4.« less

MAP Attitude Control System Design and Analysis

NASA Technical Reports Server (NTRS)

Andrews, S. F.; Campbell, C. E.; Ericsson-Jackson, A. J.; Markley, F. L.; ODonnell, J. R., Jr.

1997-01-01

The Microwave Anisotropy Probe (MAP) is a follow-on to the Differential Microwave Radiometer (DMR) instrument on the Cosmic Background Explorer (COBE) spacecraft. The MAP spacecraft will perform its mission in a Lissajous orbit around the Earth-Sun L(sub 2) Lagrange point to suppress potential instrument disturbances. To make a full-sky map of cosmic microwave background fluctuations, a combination fast spin and slow precession motion will be used. MAP requires a propulsion system to reach L(sub 2), to unload system momentum, and to perform stationkeeping maneuvers once at L(sub 2). A minimum hardware, power and thermal safe control mode must also be provided. Sufficient attitude knowledge must be provided to yield instrument pointing to a standard deviation of 1.8 arc-minutes. The short development time and tight budgets require a new way of designing, simulating, and analyzing the Attitude Control System (ACS). This paper presents the design and analysis of the control system to meet these requirements.

Same-day 2-L PEG-citrate-simethicone plus bisacodyl vs split 4-L PEG: Bowel cleansing for late-morning colonoscopy.

PubMed

de Leone, Annalisa; Tamayo, Darina; Fiori, Giancarla; Ravizza, Davide; Trovato, Cristina; De Roberto, Giuseppe; Fazzini, Linda; Dal Fante, Marco; Crosta, Cristiano

2013-09-16

To evaluate the efficacy, tolerability, acceptability and feasibility of bisacodyl plus low volume polyethyleneglycol-citrate-simeticone (2-L PEG-CS) taken the same day as compared with conventional split-dose 4-L PEG for late morning colonoscopy. Randomised, observer-blind, parallel group, comparative trial carried out in 2 centres. Out patients of both sexes, aged between 18 and 85 years, undergoing colonoscopy for diagnostic investigation, colorectal cancer screening or follow-up were eligible. The PEG-CS group received 3 bisacodyl tablets (4 tablets for patients with constipation) at bedtime and 2-L PEG-CS in the morning starting 5 h before colonoscopy. The control group received a conventional 4-L PEG formulation given as split regimen; the morning dose was taken with the same schedule of the low volume preparation. The Ottawa Bowel Preparation Scale (OBPS) score was used as the main outcome measure. A total of 164 subjects were enrolled and 154 completed the study; 78 in the PEG-CS group and 76 in the split 4-L PEG group. The two groups were comparable at baseline. The OBPS score in the PEG-CS group (3.09 ± 2.40) and in the PEG group (2.39 ± 2.55) were equivalent (difference +0.70; 95%CI: -0.09-1.48). This was confirmed by the rate of successful bowel cleansing in the PEG-CS group (89.7%) and in the PEG group (92.1%) (difference -2.4%; 95%CI: -11.40- 6.70). PEG-CS was superior in terms of mucosa visibility compared to PEG (85.7% vs 72.4%, P = 0.042). There were no significant differences in caecum intubation rate, time to reach the caecum and withdrawal time between the two groups. The adenoma detection rate was similar (PEG-CS 43.6% vs PEG 44.7%). No serious adverse events occurred. No difference was found in tolerability of the bowel preparations. Compliance was equal in both groups: more than 90% of subjects drunk the whole solution. Willingness to repeat the same bowel preparations was about 90% for both regimes. Same-day PEG-CS is feasible, effective

Development and Evaluation of a Hydrostatic Dynamical Core Using the Spectral Element/Discontinuous Galerkin Methods

DTIC Science & Technology

2014-04-01

The CG and DG horizontal discretization employs high-order nodal basis functions associated with Lagrange polynomials based on Gauss-Lobatto- Legendre ...and DG horizontal discretization employs high-order nodal basis functions 29 associated with Lagrange polynomials based on Gauss-Lobatto- Legendre ...Inside 235 each element we build ( 1)N + Gauss-Lobatto- Legendre (GLL) quadrature points, where N 236 indicate the polynomial order of the basis

Reactions of l-ergothioneine and some other aminothiones with 2,2′- and 4,4′-dipyridyl disulphides and of l-ergothioneine with iodoacetamide. 2-Mercaptoimidazoles, 2- and 4-thiopyridones, thiourea and thioacetamide as highly reactive neutral sulphur nucleophiles

PubMed Central

Carlsson, Jan; Kierstan, Marek P. J.; Brocklehurst, Keith

1974-01-01

1. The reactions of 2,2′- and 4,4′-dipyridyl disulphide (2-Py–S–S–2-Py and 4-Py–S–S–4-Py) with l-ergothioneine (2-mercapto-l-histidine betaine), 2-mercaptoimidazole, 1-methyl-2-mercaptoimidazole, thiourea, thioacetamide, 2-thiopyridone (Py–2-SH) and 4-thiopyridone (Py–4-SH) were investigated spectrophotometrically in the pH range approx. 1–9. 2. These reactions involve two sequential reversible thiol–disulphide interchanges. 3. The reaction of l-ergothioneine with 2-Py–S–S–2-Py and/or with the l-ergothioneine–Py–2-SH mixed disulphide, both of which provide Py–2-SH, is characterized by at least three reactive protonic states. This provides definitive evidence that neutral l-ergothioneine is a reactive nucleophile, particularly towards the highly electrophilic protonated disulphides. 4. A similar situation appears to obtain in the reactions of l-ergothioneine and Py–2-SH with 4-Py–S–S–4-Py and in the reactions of the other 2-mercaptoimidazoles, thiourea and Py–4-SH with 2-Py–S–S–2-Py. The nucleophilic reactivity of Py–4-SH suggests that general base catalysis provided by the disulphide in a cyclic or quasi-cyclic transition state is not necessary to generate nucleophilic reactivity in the other amino-thiones whose geometry could permit such catalysis. 5. The existence of a positive deuterium isotope effect in the l-ergothioneine–2-Py–S–S–2-Py system at pH6–7 provides no evidence for general base catalysis but is in accord with a mechanism involving specific acid catalysis and post-transition-state proton transfer. 6. The pH-dependences of the overall equilibrium positions of the various thiol–disulphide interchanges are described. 7. Reaction of thioacetamide with a stoicheiometric quantity of 2-Py–S–S–2-Py at pH1 provides 2 molecules of Py–2-SH per molecule of thioacetamide and elemental sulphur; these findings can be accounted for by thiol–disulphide interchange to provide a thioacetamide�

Soil Moisture Active Passive Mission L4_C Data Product Assessment (Version 2 Validated Release)

NASA Technical Reports Server (NTRS)

Kimball, John S.; Jones, Lucas A.; Glassy, Joseph; Stavros, E. Natasha; Madani, Nima; Reichle, Rolf H.; Jackson, Thomas; Colliander, Andreas

2016-01-01

The SMAP satellite was successfully launched January 31st 2015, and began acquiring Earth observation data following in-orbit sensor calibration. Global data products derived from the SMAP L-band microwave measurements include Level 1 calibrated and geolocated radiometric brightness temperatures, Level 23 surface soil moisture and freezethaw geophysical retrievals mapped to a fixed Earth grid, and model enhanced Level 4 data products for surface to root zone soil moisture and terrestrial carbon (CO2) fluxes. The post-launch SMAP mission CalVal Phase had two primary objectives for each science product team: 1) calibrate, verify, and improve the performance of the science algorithms, and 2) validate accuracies of the science data products as specified in the L1 science requirements. This report provides analysis and assessment of the SMAP Level 4 Carbon (L4_C) product pertaining to the validated release. The L4_C validated product release effectively replaces an earlier L4_C beta-product release (Kimball et al. 2015). The validated release described in this report incorporates a longer data record and benefits from algorithm and CalVal refinements acquired during the SMAP post-launch CalVal intensive period. The SMAP L4_C algorithms utilize a terrestrial carbon flux model informed by SMAP soil moisture inputs along with optical remote sensing (e.g. MODIS) vegetation indices and other ancillary biophysical data to estimate global daily net ecosystem CO2 exchange (NEE) and component carbon fluxes for vegetation gross primary production (GPP) and ecosystem respiration (Reco). Other L4_C product elements include surface (10 cm depth) soil organic carbon (SOC) stocks and associated environmental constraints to these processes, including soil moisture and landscape freeze/thaw (FT) controls on GPP and respiration (Kimball et al. 2012). The L4_C product encapsulates SMAP carbon cycle science objectives by: 1) providing a direct link between terrestrial carbon fluxes and

Y4lO of Rhizobium sp. Strain NGR234 Is a Symbiotic Determinant Required for Symbiosome Differentiation▿

PubMed Central

Yang, Feng-Juan; Cheng, Li-Li; Zhang, Ling; Dai, Wei-Jun; Liu, Zhe; Yao, Nan; Xie, Zhi-Ping; Staehelin, Christian

2009-01-01

Type 3 (T3) effector proteins, secreted by nitrogen-fixing rhizobia with a bacterial T3 secretion system, affect the nodulation of certain host legumes. The open reading frame y4lO of Rhizobium sp. strain NGR234 encodes a protein with sequence similarities to T3 effectors from pathogenic bacteria (the YopJ effector family). Transcription studies showed that the promoter activity of y4lO depended on the transcriptional activator TtsI. Recombinant Y4lO protein expressed in Escherichia coli did not acetylate two representative mitogen-activated protein kinase kinases (human MKK6 and MKK1 from Medicago truncatula), indicating that YopJ-like proteins differ with respect to their substrate specificities. The y4lO gene was mutated in NGR234 (strain NGRΩy4lO) and in NGRΩnopL, a mutant that does not produce the T3 effector NopL (strain NGRΩnopLΩy4lO). When used as inoculants, the symbiotic properties of the mutants differed. Tephrosia vogelii, Phaseolus vulgaris cv. Yudou No. 1, and Vigna unguiculata cv. Sui Qing Dou Jiao formed pink effective nodules with NGR234 and NGRΩnopLΩy4lO. Nodules induced by NGRΩy4lO were first pink but rapidly turned greenish (ineffective nodules), indicating premature senescence. An ultrastructural analysis of the nodules induced by NGRΩy4lO revealed abnormal formation of enlarged infection droplets in ineffective nodules, whereas symbiosomes harboring a single bacteroid were frequently observed in effective nodules induced by NGR234 or NGRΩnopLΩy4lO. It is concluded that Y4lO is a symbiotic determinant involved in the differentiation of symbiosomes. Y4lO mitigated senescence-inducing effects caused by the T3 effector NopL, suggesting synergistic effects for Y4lO and NopL in nitrogen-fixing nodules. PMID:19060155

4. Historic American Buildings Survey L. C. Durette, Photographer March ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

4. Historic American Buildings Survey L. C. Durette, Photographer March 19, 1937 EAST ROOM FIRST FLOOR (MEAL ROOM) CHUTES, CORN MEAL BIN + SIEVE - Winnicut Grist Mill, Winnicut River, Stratham, Rockingham County, NH

Primary photochemical processes in fac-ClRe(CO){sub 3}L{sub 2} (L = 4-phenylpyridine and 4-cyanopyridine): A steady-state and flash photochemical study of reaction products and intermediates

DOE Office of Scientific and Technical Information (OSTI.GOV)

Feliz, M.; Ferraudi, G.; Altmiller, H.

1992-01-09

The photochemistry of fac-ClRe(CO){sub 3}L{sub 2}, L = 4-phenylpyridine and 4-cyanopyridine, has been investigated by monochromatic steady-state and flash photolyses between 400 and 229 nm. Two parallel photoprocesses, the photogeneration of the emissive MLCT state and the photoredox dissociation in (ClRe(CO){sub 3}L{sup +}, L{sup {sm_bullet}{minus}}) products, have been observed with both compounds. A third photoprocess, namely, the photogeneration of a Re(I)-ligand biradical, has been observed only in photolyses of the 4-phenylpyridine complex. While this Re(I)-ligand biradical reduces Cu{sup II}(TIM){sup 2+} to the corresponding Cu(I) species, no such reaction is undergone by the MLCT state. Differences between the electronic structures ofmore » these complexes, shown by extended Hueckel MO calculations, were related to their intrinsic photochemical behavior. 54 refs., 9 figs., 1 tab.« less

Searching for artificial equilibrium points to place satellites "above and below" L3 in the Sun-Earth system

NASA Astrophysics Data System (ADS)

de Almeida, A. K., Jr.; Prado, A. F. B. A.; Sanchez, D. M.; Yokoyama, T.

2017-10-01

Regarding practical applications of L3 of the Sun-Earth system, there are few studies with the goal of placing a spacecraft at this point, or in orbit around it. One of the main problems in placing a spacecraft near this equilibrium point is the fact that it is located behind the Sun with respect to the Earth. The Sun would be blocking direct communication between the spacecraft and the Earth. The present research gives several options to solve this problem by using a solar sail to place one or two spacecraft above and/or below the Ecliptic plane. This sail could also be used for the mission itself, to collect energy or particles. By using an adequate size, location and attitude of the solar sail, the equilibrium point can be moved from its original location to allow communications between the spacecraft and the Earth. A preliminary study of a solar sail that uses this strategy is shown here.

Chaotic Dynamics in a Low-Energy Transfer Strategy to the Equilateral Equilibrium Points in the Earth-Moon System

NASA Astrophysics Data System (ADS)

Salazar, F. J. T.; Macau, E. E. N.; Winter, O. C.

In the frame of the equilateral equilibrium points exploration, numerous future space missions will require maximization of payload mass, simultaneously achieving reasonable transfer times. To fulfill this request, low-energy non-Keplerian orbits could be used to reach L4 and L5 in the Earth-Moon system instead of high energetic transfers. Previous studies have shown that chaos in physical systems like the restricted three-body Earth-Moon-particle problem can be used to direct a chaotic trajectory to a target that has been previously considered. In this work, we propose to transfer a spacecraft from a circular Earth Orbit in the chaotic region to the equilateral equilibrium points L4 and L5 in the Earth-Moon system, exploiting the chaotic region that connects the Earth with the Moon and changing the trajectory of the spacecraft (relative to the Earth) by using a gravity assist maneuver with the Moon. Choosing a sequence of small perturbations, the time of flight is reduced and the spacecraft is guided to a proper trajectory so that it uses the Moon's gravitational force to finally arrive at a desired target. In this study, the desired target will be an orbit about the Lagrangian equilibrium points L4 or L5. This strategy is not only more efficient with respect to thrust requirement, but also its time transfer is comparable to other known transfer techniques based on time optimization.

U.S. EPA, Pesticide Product Label, GOWAN PROMETRYNE 4L, 02/02/1990

EPA Pesticide Factsheets

2011-04-21

... l·to'"TI('''· I' t..'('lr::'· t-!!') tI-( f;~1 ~rFt Sl·.4~~f" P"'c' ,~ : _'" _ 1't,..."i(C orr'" C'''f'' po:: ... 1,'1'~"". 0' f ",~"'tt:: ~~, l"eC" .... ... l ; . " . ~IOO"'I,so"d DOM'''''f' ...

Angiostatic, tumor inflammatory and anti-tumor effects of CXCL4(47-70) and CXCL4L1(47-70) in an EGF-dependent breast cancer model.

PubMed

Van Raemdonck, Katrien; Berghmans, Nele; Vanheule, Vincent; Bugatti, Antonella; Proost, Paul; Opdenakker, Ghislain; Presta, Marco; Van Damme, Jo; Struyf, Sofie

2014-11-15

CXCL4 and CXCL4L1, platelet-derived CXC chemokines, and their carboxy-terminal peptides CXCL4(47-70) and CXCL4L1(47-70) previously displayed angiostatic and anti-tumoral activity in a melanoma model. Here, we found CXCL4(47-70) and CXCL4L1(47-70) to inhibit lymphatic endothelial cell proliferation in vitro. Furthermore, the angiostatic potential of CXCL4(47-70) and CXCL4L1(47-70) was tested against different angiogenic stimuli (FGF1, FGF2, FGF8, EGF and VEGF). Besides reducing FGF2-induced vascular endothelial cell growth, CXCL4(47-70) and CXCL4L1(47-70) efficiently counteracted EGF. Consequently, we considered their anti-tumoral potential in EGF-dependent MDA-MB-231 breast tumors. In tumor-bearing mice, CXCL4(47-70) reduced tumor growth better than CXCL4L1(47-70). In CXCL4(47-70)-treated tumors significantly more intratumoral monocytes/macrophages and dendritic cells were present and higher expression levels of CCL5 and IFN- γ were detected by qPCR on tumor lysates. Because neither peptide was able to specifically bind CXCR3A or CXCR3B, differential glycosaminoglycan binding and direct interaction with cytokines (EGF and CCL5) might explain any differences in anti-tumoral effects. Notably, CCL5-induced monocyte chemotaxis in vitro was increased by addition of CXCL4(47-70) or CXCL4L1(47-70). Finally, CXCL4(47-70) and CXCL4L1(47-70) inhibited proliferation of MDA-MB-231 cells. Our results suggest a tumor type-dependent responsiveness to either CXCL4(47-70) or CXCL4L1(47-70) treatment, defined by anti-proliferative, angiostatic and inflammatory actions, and substantiate their therapeutic potential.

Subcomponent self-assembly and guest-binding properties of face-capped Fe4L4(8+) capsules.

PubMed

Bilbeisi, Rana A; Clegg, Jack K; Elgrishi, Noémie; de Hatten, Xavier; Devillard, Marc; Breiner, Boris; Mal, Prasenjit; Nitschke, Jonathan R

2012-03-21

A general method for preparing Fe(4)L(4) face-capped tetrahedral cages through subcomponent self-assembly was developed and has been demonstrated using four different C(3)-symmetric triamines, 2-formylpyridine, and iron(II). Three of the triamines were shown also to form Fe(2)L(3) helicates when the appropriate stoichiometry of subcomponents was used. Two of the cages were observed to have nearly identical Fe-Fe distances in the solid state, which enabled their ligands to be coincorporated into a collection of mixed cages. Only one of the cages combined a sufficiently large cavity with the sufficiently small pores required for guest binding, taking up a wide variety of guest species in size- and shape-selective fashion.

Synthesis of novel C-4'-spiro-oxetano-α-L-ribonucleosides.

PubMed

Kumar, Rajesh; Kumar, Manish; Singh, Ankita; Singh, Neetu; Maity, Jyotirmoy; Prasad, Ashok K

2017-06-05

The synthesis of novel C-4'-spiro-oxetano-α-L-ribonucleosides T and U in 39 and 45% overall yields have been achieved from 2',3',5'-tri-O-acetyl-4'-C-p-toluenesulfonyloxymethyl-β-D-xylofuranosylthymine and 2',3',5'-tri-O-acetyl-4'-C-p-toluenesulfonyloxymethyl-β-D-xylofuranosyluracil, respectively. Both the tosylated nucleoside precursors have been synthesized following recently developed Novozyme ® -435 catalyzed methodology. Copyright © 2017 Elsevier Ltd. All rights reserved.

Active form Notch4 promotes the proliferation and differentiation of 3T3-L1 preadipocytes

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lai, Peng-Yeh; Tsai, Chong-Bin; Department of Ophthalmology, Chiayi Christian Hospital, Chiayi 600, Taiwan, ROC

2013-01-18

Highlights: ► Notch4IC modulates the ERK pathway and cell cycle to promote 3T3-L1 proliferation. ► Notch4IC facilitates 3T3-L1 differentiation by up-regulating proadipogenic genes. ► Notch4IC promotes proliferation during the early stage of 3T3-L1 adipogenesis. ► Notch4IC enhances differentiation during subsequent stages of 3T3-L1 adipogenesis. -- Abstract: Adipose tissue is composed of adipocytes, which differentiate from precursor cells in a process called adipogenesis. Many signal molecules are involved in the transcriptional control of adipogenesis, including the Notch pathway. Previous adipogenic studies of Notch have focused on Notch1 and HES1; however, the role of other Notch receptors in adipogenesis remains unclear. Q-RT-PCRmore » analyses showed that the augmentation of Notch4 expression during the differentiation of 3T3-L1 preadipocytes was comparable to that of Notch1. To elucidate the role of Notch4 in adipogenesis, the human active form Notch4 (N4IC) was transiently transfected into 3T3-L1 cells. The expression of HES1, Hey1, C/EBPδ and PPARγ was up-regulated, and the expression of Pref-1, an adipogenic inhibitor, was down-regulated. To further characterize the effect of N4IC in adipogenesis, stable cells expressing human N4IC were established. The expression of N4IC promoted proliferation and enhanced differentiation of 3T3-L1 cells compared with those of control cells. These data suggest that N4IC promoted proliferation through modulating the ERK pathway and the cell cycle during the early stage of 3T3-L1 adipogenesis and facilitated differentiation through up-regulating adipogenic genes such as C/EBPα, PPARγ, aP2, LPL and HSL during the middle and late stages of 3T3-L1 adipogenesis.« less

LPJmL4 - a dynamic global vegetation model with managed land - Part 1: Model description

NASA Astrophysics Data System (ADS)

Schaphoff, Sibyll; von Bloh, Werner; Rammig, Anja; Thonicke, Kirsten; Biemans, Hester; Forkel, Matthias; Gerten, Dieter; Heinke, Jens; Jägermeyr, Jonas; Knauer, Jürgen; Langerwisch, Fanny; Lucht, Wolfgang; Müller, Christoph; Rolinski, Susanne; Waha, Katharina

2018-04-01

This paper provides a comprehensive description of the newest version of the Dynamic Global Vegetation Model with managed Land, LPJmL4. This model simulates - internally consistently - the growth and productivity of both natural and agricultural vegetation as coherently linked through their water, carbon, and energy fluxes. These features render LPJmL4 suitable for assessing a broad range of feedbacks within and impacts upon the terrestrial biosphere as increasingly shaped by human activities such as climate change and land use change. Here we describe the core model structure, including recently developed modules now unified in LPJmL4. Thereby, we also review LPJmL model developments and evaluations in the field of permafrost, human and ecological water demand, and improved representation of crop types. We summarize and discuss LPJmL model applications dealing with the impacts of historical and future environmental change on the terrestrial biosphere at regional and global scale and provide a comprehensive overview of LPJmL publications since the first model description in 2007. To demonstrate the main features of the LPJmL4 model, we display reference simulation results for key processes such as the current global distribution of natural and managed ecosystems, their productivities, and associated water fluxes. A thorough evaluation of the model is provided in a companion paper. By making the model source code freely available at L" target="_blank">https://gitlab.pik-potsdam.de/lpjml/LPJmL, we hope to stimulate the application and further development of LPJmL4 across scientific communities in support of major activities such as the IPCC and SDG process.

Point-of-care CD4 testing to inform selection of antiretroviral medications in south african antenatal clinics: a cost-effectiveness analysis.

PubMed

Ciaranello, Andrea L; Myer, Landon; Kelly, Kathleen; Christensen, Sarah; Daskilewicz, Kristen; Doherty, Katie; Bekker, Linda-Gail; Hou, Taige; Wood, Robin; Francke, Jordan A; Wools-Kaloustian, Kara; Freedberg, Kenneth A; Walensky, Rochelle P

2015-01-01

Many prevention of mother-to-child HIV transmission (PMTCT) programs currently prioritize antiretroviral therapy (ART) for women with advanced HIV. Point-of-care (POC) CD4 assays may expedite the selection of three-drug ART instead of zidovudine, but are costlier than traditional laboratory assays. We used validated models of HIV infection to simulate pregnant, HIV-infected women (mean age 26 years, gestational age 26 weeks) in a general antenatal clinic in South Africa, and their infants. We examined two strategies for CD4 testing after HIV diagnosis: laboratory (test rate: 96%, result-return rate: 87%, cost: $14) and POC (test rate: 99%, result-return rate: 95%, cost: $26). We modeled South African PMTCT guidelines during the study period (WHO "Option A"): antenatal zidovudine (CD4 ≤350/μL) or ART (CD4>350/μL). Outcomes included MTCT risk at weaning (age 6 months), maternal and pediatric life expectancy (LE), maternal and pediatric lifetime healthcare costs (2013 USD), and cost-effectiveness ($/life-year saved). In the base case, laboratory led to projected MTCT risks of 5.7%, undiscounted pediatric LE of 53.2 years, and undiscounted PMTCT plus pediatric lifetime costs of $1,070/infant. POC led to lower modeled MTCT risk (5.3%), greater pediatric LE (53.4 years) and lower PMTCT plus pediatric lifetime costs ($1,040/infant). Maternal outcomes following laboratory were similar to POC (LE: 21.2 years; lifetime costs: $23,860/person). Compared to laboratory, POC improved clinical outcomes and reduced healthcare costs. In antenatal clinics implementing Option A, the higher initial cost of a one-time POC CD4 assay will be offset by cost-savings from prevention of pediatric HIV infection.

Professional Military Education for Life (PME4L)

DTIC Science & Technology

2014-12-08

The Air Force embracing a continuous educational model which integrates CPE concepts in order to persistently develop professional Airmen, engages...exposure to new ideas and career fields. Due to PME’s importance in an Airman’s career development, PME should be a continuous process with the...current PME courses as anchors. PME4L complements traditional Air Force PME and invests in Airmen at all levels. This paper presents a Continuous

Shear-wave seismic reflection imaging and impedance inversion for a near-surface point-bar

NASA Astrophysics Data System (ADS)

Benton, N. W.; Morrison, M.; Lorenzo, J. M.; Odom, B.; Clift, P. D.; Olson, E.; Gostic, A.

2017-12-01

Imaging and inversion of SH-waves are useful to detect, map, and quantitatively characterize near-surface point-bar strata. We conduct a horizontally-polarized (SH) reflection survey across and along a near-surface (9 - 40 m) downstream point-bar. We invert for shear-impedance profiles and correlate our interpretation to electrical conductivity (EC) logs in adjacent wells to study the internal architecture and lithology of point-bars. We acquire two common-midpoint (CMP) SH-wave seismic reflection lines at False River (Point Coupee Parish, Louisiana). A 104 m long seismic line (L1) is oriented orthogonal (NW - SE) to point-bar strike. A second line (L2) is 48 m long and set parallel to point-bar strike (NE - SW). Two EC wells lie 33 m apart. Both wells are parallel with respect to the L1 survey and offset from it by 15 m. EC log measurements range from 1 - 25 m depth. Interference of Love-waves prevents seismic imaging at depths less than 9 m. The L1 and L2 data sets are inverted for shear-impedance using a model-based band-limited impedance (BLIMP) algorithm that incorporates a low-frequency velocity model. This model is also used for the depthing processing. The L1 cross-section shows coherent dipping reflection events ( 4 - 7º) from 0.15 - 0.35 s (10 - 40 m). The corresponding shear-impedance profile also reveals coherent and dipping impedance contrasts that grow in magnitude with increasing depth. The L2 cross-section shows comparatively less dip ( 1º) as well as sharper and shallower continuity of reflection events (0.1 - 0.28 s TWT or 9 - 25 m). Depth-converted (TVD) seismic amplitudes and impedance values correlate to near-surface point-bar geology via superposition of log data. The first well (W5) shows distinct EC local maxima (+50 - 70 mS/m) at 14.5 and 15.5 m depth that correlate well with the seismic amplitudes and impedance values from both L1 and L2 data sets. The second well (W7) shows comparatively lower local maxima (+40 - 60 mS/m) but at greater

Plasmonic Roche lobe in metal-dielectric-metal structure

DOE Office of Scientific and Technical Information (OSTI.GOV)

Shiu, Ruei-Cheng; Lan, Yung-Chiang

2013-07-15

This study investigates a plasmonic Roche lobe that is based on a metal-dielectric-metal (MDM) structure using finite-difference time-domain simulations and theoretical analyses. The effective refractive index of the MDM structure has two centers and is inversely proportional to the distance from the position of interest to the centers, in a manner that is analogous to the gravitational potential in a two-star system. The motion of surface plasmons (SPs) strongly depends on the ratio of permittivities at the two centers. The Lagrange point is an unstable equilibrium point for SPs that propagate in the system. After the SPs have passed throughmore » the Lagrange point, their spread drastically increases.« less

75 FR 34776 - Florida Power & Light Company; Turkey Point Nuclear Generating Plant, Units 3 and 4...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-18

... changes to the reactor, fuel, plant, structures, support structures, water, or land at the Turkey Point... NUCLEAR REGULATORY COMMISSION [Docket Nos. 50-250 and 50-251; NRC-2010-0212] Florida Power & Light Company; Turkey Point Nuclear Generating Plant, Units 3 and 4; Environmental Assessment and Finding of No...

Same-day 2-L PEG-citrate-simethicone plus bisacodyl vs split 4-L PEG: Bowel cleansing for late-morning colonoscopy

PubMed Central

de Leone, Annalisa; Tamayo, Darina; Fiori, Giancarla; Ravizza, Davide; Trovato, Cristina; De Roberto, Giuseppe; Fazzini, Linda; Dal Fante, Marco; Crosta, Cristiano

2013-01-01

AIM: To evaluate the efficacy, tolerability, acceptability and feasibility of bisacodyl plus low volume polyethyleneglycol-citrate-simeticone (2-L PEG-CS) taken the same day as compared with conventional split-dose 4-L PEG for late morning colonoscopy. METHODS: Randomised, observer-blind, parallel group, comparative trial carried out in 2 centres. Out patients of both sexes, aged between 18 and 85 years, undergoing colonoscopy for diagnostic investigation, colorectal cancer screening or follow-up were eligible. The PEG-CS group received 3 bisacodyl tablets (4 tablets for patients with constipation) at bedtime and 2-L PEG-CS in the morning starting 5 h before colonoscopy. The control group received a conventional 4-L PEG formulation given as split regimen; the morning dose was taken with the same schedule of the low volume preparation. The Ottawa Bowel Preparation Scale (OBPS) score was used as the main outcome measure. RESULTS: A total of 164 subjects were enrolled and 154 completed the study; 78 in the PEG-CS group and 76 in the split 4-L PEG group. The two groups were comparable at baseline. The OBPS score in the PEG-CS group (3.09 ± 2.40) and in the PEG group (2.39 ± 2.55) were equivalent (difference +0.70; 95%CI: -0.09-1.48). This was confirmed by the rate of successful bowel cleansing in the PEG-CS group (89.7%) and in the PEG group (92.1%) (difference -2.4%; 95%CI: -11.40- 6.70). PEG-CS was superior in terms of mucosa visibility compared to PEG (85.7% vs 72.4%, P = 0.042). There were no significant differences in caecum intubation rate, time to reach the caecum and withdrawal time between the two groups. The adenoma detection rate was similar (PEG-CS 43.6% vs PEG 44.7%). No serious adverse events occurred. No difference was found in tolerability of the bowel preparations. Compliance was equal in both groups: more than 90% of subjects drunk the whole solution. Willingness to repeat the same bowel preparations was about 90% for both regimes. CONCLUSION: Same

The roles and potential therapeutic implications of CXCL4 and its variant CXCL4L1 in the pathogenesis of chronic liver allograft dysfunction.

PubMed

Li, Jing; Liu, Bin; Yan, Lu-nan; Lau, Wan-yee

2015-02-01

Chronic liver allograft dysfunction is the leading cause of patient morbidity and late allograft loss after liver transplantation. The pathogenesis of chronic liver allograft dysfunction remains unknown. Recent studies have demonstrated that CXCL4 and its variant CXCL4L1 are involved in organ damage induced through inflammatory and immune responses throughout all stages of liver transplantation. CXCL4 and CXCL4L1 are low-molecular-weight proteins that have been implicated in hematopoiesis, angiostasis, organ fibrogenesis, mitogenesis, tumor growth and metastasis. The purpose of this review is to discuss the current status and future developments of research into the roles of CXCL4 and CXCL4L1 in the pathogenesis of chronic liver allograft dysfunction. The potential utilization of CXCL4 and CXCL4L1 as therapeutic targets for chronic liver allograft dysfunction will also be discussed. Copyright © 2014 Elsevier Ltd. All rights reserved.

A finite element model of the L4-L5-S1 human spine segment including the heterogeneity and anisotropy of the discs.

PubMed

Jaramillo, Hector E; Gómez, Lessby; García, Jose J

2015-01-01

With the aim to study disc degeneration and the risk of injury during occupational activities, a new finite element (FE) model of the L4-L5-S1 segment of the human spine was developed based on the anthropometry of a typical Colombian worker. Beginning with medical images, the programs CATIA and SOLIDWORKS were used to generate and assemble the vertebrae and create the soft structures of the segment. The software ABAQUS was used to run the analyses, which included a detailed model calibration using the experimental step-wise reduction data for the L4-L5 component, while the L5-S1 segment was calibrated in the intact condition. The range of motion curves, the intradiscal pressure and the lateral bulging under pure moments were considered for the calibration. As opposed to other FE models that include the L5-S1 disc, the model developed in this study considered the regional variations and anisotropy of the annulus as well as a realistic description of the nucleus geometry, which allowed an improved representation of experimental data during the validation process. Hence, the model can be used to analyze the stress and strain distributions in the L4-L5 and L5-S1 discs of workers performing activities such as lifting and carrying tasks.

Adaptation of an L-proline adenylation domain to use 4-propyl-L-proline in the evolution of lincosamide biosynthesis.

PubMed

Kadlčík, Stanislav; Kučera, Tomáš; Chalupská, Dominika; Gažák, Radek; Koběrská, Markéta; Ulanová, Dana; Kopecký, Jan; Kutejová, Eva; Najmanová, Lucie; Janata, Jiří

2013-01-01

Clinically used lincosamide antibiotic lincomycin incorporates in its structure 4-propyl-L-proline (PPL), an unusual amino acid, while celesticetin, a less efficient related compound, makes use of proteinogenic L-proline. Biochemical characterization, as well as phylogenetic analysis and homology modelling combined with the molecular dynamics simulation were employed for complex comparative analysis of the orthologous protein pair LmbC and CcbC from the biosynthesis of lincomycin and celesticetin, respectively. The analysis proved the compared proteins to be the stand-alone adenylation domains strictly preferring their own natural substrate, PPL or L-proline. The LmbC substrate binding pocket is adapted to accommodate a rare PPL precursor. When compared with L-proline specific ones, several large amino acid residues were replaced by smaller ones opening a channel which allowed the alkyl side chain of PPL to be accommodated. One of the most important differences, that of the residue corresponding to V306 in CcbC changing to G308 in LmbC, was investigated in vitro and in silico. Moreover, the substrate binding pocket rearrangement also allowed LmbC to effectively adenylate 4-butyl-L-proline and 4-pentyl-L-proline, substrates with even longer alkyl side chains, producing more potent lincosamides. A shift of LmbC substrate specificity appears to be an integral part of biosynthetic pathway adaptation to the PPL acquisition. A set of genes presumably coding for the PPL biosynthesis is present in the lincomycin--but not in the celesticetin cluster; their homologs are found in biosynthetic clusters of some pyrrolobenzodiazepines (PBD) and hormaomycin. Whereas in the PBD and hormaomycin pathways the arising precursors are condensed to another amino acid moiety, the LmbC protein is the first functionally proved part of a unique condensation enzyme connecting PPL to the specialized amino sugar building unit.

The [4Fe-4S](2+) cluster in reconstituted biotin synthase binds S-adenosyl-L-methionine.

PubMed

Cosper, Michele Mader; Jameson, Guy N L; Davydov, Roman; Eidsness, Marly K; Hoffman, Brian M; Huynh, Boi Hanh; Johnson, Michael K

2002-11-27

The combination of resonance Raman, electron paramagnetic resonance and Mössbauer spectroscopies has been used to investigate the effect of S-adenosyl-l-methionine (SAM) on the spectroscopic properties of the [4Fe-4S]2+ cluster in biotin synthase. The results indicate that SAM interacts directly at a unique iron site of the [4Fe-4S]2+ cluster in BioB and support the hypothesis of a common inner-sphere mechanism for the reductive cleavage of SAM in the radical SAM family of Fe-S enzymes.

[VALUE OF SMART PHONE Scoliometer SOFTWARE IN OBTAINING OPTIMAL LUMBAR LORDOSIS DURING L4-S1 FUSION SURGERY].

PubMed

Yu, Weibo; Liang, De; Ye, Linqiang; Jiang, Xiaobing; Yao, Zhensong; Tang, Jingjing; Tang, Yongchao

2015-10-01

To investigate the value of smart phone Scoliometer software in obtaining optimal lumbar lordosis (LL) during L4-S1 fusion surgery. Between November 2014 and February 2015, 20 patients scheduled for L4-S1 fusion surgery were prospectively enrolled the study. There were 8 males and 12 females, aged 41-65 years (mean, 52.3 years). The disease duration ranged from 6 months to 6 years (mean, 3.4 years). Before operation, the pelvic incidence (PI) and Cobb angle of L4-S1 (CobbL4-S1) were measured on lateral X-ray film of lumbosacral spine by PACS system; and the ideal CobbL4-S1 was then calculated according to previously published methods [(PI+9 degrees) x 70%]. Subsequently, intraoperative CobbL4-S1 was monitored by the Scoliometer software and was defined as optimal while it was less than 5 degrees difference compared with ideal CobbL4-S1. Finally, the CobbL4-S1 was measured by the PACS system after operation and the consistency was compared between Scoliometer software and PACS system to evaluate the accuracy of this software. In addition, value of this method in obtaining optimal LL was validated by comparing the difference between ideal CobbL4-S1 and preoperative one with that between ideal CobbL4-S1 and postoperative one. The CobbL4-S1 was (36.17 ± 1.53)degrees for ideal one, (22.57 ± 5.50)degrees for preoperative one, (32.25 ± 1.46)degrees for intraoperative one measured by Scoliometer software, and (34.43 ± 1.72)degrees for postoperative one, respectively. The observed intraclass correlation coefficient (ICC) was excellent [ICC = 0.96, 95% confidence interval (0.93, 0.97)] and the mean absolute difference (MAD) was low (MAD = 1.23) between Scoliometer software and PACS system. The deviation between ideal CobbL4-S1 and postoperative CobbL4-S1 was (2.31 ± 0.23)degrees, which was significantly lower than the deviation between ideal CobbL4-S1 and preoperative CobbL4-S1 (13.60 ± 1.85)degrees (t = 6.065, P = 0.001). Scoliometer software can help surgeon obtain

f( R, L m ) gravity

NASA Astrophysics Data System (ADS)

Harko, Tiberiu; Lobo, Francisco S. N.

2010-11-01

We generalize the f( R) type gravity models by assuming that the gravitational Lagrangian is given by an arbitrary function of the Ricci scalar R and of the matter Lagrangian L m . We obtain the gravitational field equations in the metric formalism, as well as the equations of motion for test particles, which follow from the covariant divergence of the energy-momentum tensor. The equations of motion for test particles can also be derived from a variational principle in the particular case in which the Lagrangian density of the matter is an arbitrary function of the energy density of the matter only. Generally, the motion is non-geodesic, and it takes place in the presence of an extra force orthogonal to the four-velocity. The Newtonian limit of the equation of motion is also considered, and a procedure for obtaining the energy-momentum tensor of the matter is presented. The gravitational field equations and the equations of motion for a particular model in which the action of the gravitational field has an exponential dependence on the standard general relativistic Hilbert-Einstein Lagrange density are also derived.

Complete genomic sequences for hepatitis C virus subtypes 4b, 4c, 4d, 4g, 4k, 4l, 4m, 4n, 4o, 4p, 4q, 4r and 4t.

PubMed

Li, Chunhua; Lu, Ling; Wu, Xianghong; Wang, Chuanxi; Bennett, Phil; Lu, Teng; Murphy, Donald

2009-08-01

In this study, we characterized the full-length genomic sequences of 13 distinct hepatitis C virus (HCV) genotype 4 isolates/subtypes: QC264/4b, QC381/4c, QC382/4d, QC193/4g, QC383/4k, QC274/4l, QC249/4m, QC97/4n, QC93/4o, QC139/4p, QC262/4q, QC384/4r and QC155/4t. These were amplified, using RT-PCR, from the sera of patients now residing in Canada, 11 of which were African immigrants. The resulting genomes varied between 9421 and 9475 nt in length and each contains a single ORF of 9018-9069 nt. The sequences showed nucleotide similarities of 77.3-84.3 % in comparison with subtypes 4a (GenBank accession no. Y11604) and 4f (EF589160) and 70.6-72.8 % in comparison with genotype 1 (M62321/1a, M58335/1b, D14853/1c, and 1?/AJ851228) reference sequences. These similarities were often higher than those currently defined by HCV classification criteria for subtype (75.0-80.0 %) and genotype (67.0-70.0 %) division, respectively. Further analyses of the complete and partial E1 and partial NS5B sequences confirmed these 13 'provisionally assigned subtypes'.

4. PENSTOCKS. EXHIBIT L, SANTA ANA RIVER NO. 1 PROJECT, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

4. PENSTOCKS. EXHIBIT L, SANTA ANA RIVER NO. 1 PROJECT, APR. 30, 1945. SCE drawing no. 523197 (sheet no. 7; for filing with Federal Power Commission). - Santa Ana River Hydroelectric System, SAR-1 Forebay & Penstock, Redlands, San Bernardino County, CA

Nedd4L expression is decreased in ovarian epithelial cancer tissues compared to ovarian non-cancer tissue.

PubMed

Yang, Qiuyun; Zhao, Jinghe; Cui, Manhua; Gi, Shuting; Wang, Wei; Han, Xiaole

2015-12-01

Recent studies have demonstrated that the neural precursor cell expressed, developmentally downregulated 4-like (Nedd4L) gene plays a role in the progression of various cancers. However, reports describing Nedd4L expression in ovarian cancer tissues are limited. A cohort (n = 117) of archival formalin-fixed, paraffin embedded resected normal ovarian epithelial tissues (n = 10), benign ovarian epithelial tumor tissues (n = 10), serous borderline ovarian epithelial tumor tissues (n = 14), mucous borderline ovarian epithelial tumor tissues (n = 11), and invasive ovarian epithelial cancer tissues (n = 72) were assessed for Nedd4L protein expression using immunohistochemistry. Nedd4L protein expression was significantly decreased in invasive ovarian epithelial cancer tissues compared to non-cancer tissues (P < 0.05). Decreased Nedd4L protein expression correlated with clinical stage, pathological grade, lymph node metastasis and survival (P < 0.05). Nedd4L protein expression may be an independent prognostic marker of ovarian cancer development. © 2015 Japan Society of Obstetrics and Gynecology.

A 4-week, dose-ranging study comparing the efficacy, safety and tolerability of latanoprost 75, 100 and 125 μg/mL to latanoprost 50 μg/mL (xalatan) in the treatment of primary open-angle glaucoma and ocular hypertension

PubMed Central

2012-01-01

Background Several studies have investigated the effect of latanoprost on intraocular pressure (IOP). We compared the IOP-lowering effects of three higher concentrations of latanoprost with the commercially available concentration of 0.005% (50 μg/mL) in patients with primary open-angle glaucoma or ocular hypertension. Methods Treatment-naive subjects or those receiving IOP-lowering medication with baseline IOP levels of ≥24 mmHg and ≤36 mmHg in at least one eye after washout were randomized to receive an evening dose of latanoprost 50, 75, 100, or 125 μg/mL for 4 weeks. At weeks 1, 2, 3, and 4, ocular examinations were performed and IOP was measured. Ocular symptoms and adverse events were monitored. The primary efficacy endpoint was the change in IOP from baseline to week 4 at 8 a.m. and 4 p.m. for the per protocol (PP) population using a "worse eye" analysis. Secondary efficacy endpoints were change in IOP at each time point from baseline across all visits, and percentage change in IOP from baseline to week 4 at 8 a.m. Results In all, 282 patients were randomized and treated; 274 were included in the PP population. Treatment groups were similar at baseline; 68% were diagnosed with primary open-angle glaucoma. Mean baseline IOP levels were comparable across treatments. There were no statistically significant differences in IOP reductions from baseline to week 4 at either time point between those treated with higher concentrations of latanoprost versus those receiving 50 μg/mL. Least squares mean IOP changes at 8 a.m. were −10.13, -9.59, -10.02, and −9.06 mmHg for latanoprost 50, 75, 100, and 125 μg/mL, respectively, and at 4 p.m. were −8.90, -8.29, -8.81, and −8.34 mmHg, respectively. Results of secondary efficacy analyses supported those of the primary analysis. Conjunctival hyperemia, the most commonly reported adverse event, occurred in 16.9%, 18.6%, 20.8% and 15.9% of subjects receiving latanoprost 50, 75, 100, and

4. FOREBAY AND PENSTOCK, EXHIBIT L, SANTA ANA RIVER NO. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

4. FOREBAY AND PENSTOCK, EXHIBIT L, SANTA ANA RIVER NO. 2 PROJECT, APR. 30, 1945. SCE drawing no. 523642 (sheet no. 13; for filing with the Federal Power Commission). - Santa Ana River Hydroelectric System, SAR-2 Forebay & Penstock, Redlands, San Bernardino County, CA

Reliability of the Lactate Scout point-of-care instrument for the determination of blood L-lactate concentration in sheep.

PubMed

Kaynar, Ozgur; Karapinar, Tolga; Hayirli, Armagan; Baydar, Ersoy

2015-12-01

Data on accuracy and precision of the Lactate Scout point-of-care (POC) analyzer in ovine medicine are lacking. The purpose of the study was to assess the reliability of the Lactate Scout in sheep. Fifty-seven sheep at varying ages with various diseases were included. Blood lactate concentration in samples collected from the jugular vein was measured immediately on the Lactate Scout. Plasma L-lactate concentration was measured by the Cobas autoanalyzer as the reference method. Data were subjected to Student's t-test, Passing-Bablok regression, and Bland-Altman plot analyses for comparison and assessment of accuracy, precision, and reliability. Plasma l-lactate concentration was consistently lower than blood L-lactate concentration (3.06 ± 0.24 vs 3.3 ± 0.3 mmol/L, P < .0001). There was a positive correlation between plasma and blood L-lactate concentrations (r = .98, P < .0001). The Lactate Scout had 99% accuracy and 98% precision with the reference method. Blood (Y) and plasma (X) L-lactate concentrations were fitted to Y = 0.28 + 1.00 · X, with a residual standard deviation of 0.31 and a negligible deviation from the identity line (P = .93). The bias was fitted to Y = 0.10 + 0.05 · X, with Sy.x of 0.44 (P < .07). The Lactate Scout has high accuracy and precision, with a negligible bias. It is a reliable POC analyzer to assess L-lactate concentration in ovine medicine. © 2015 American Society for Veterinary Clinical Pathology.

Parameter estimation for the 4-parameter Asymmetric Exponential Power distribution by the method of L-moments using R

USGS Publications Warehouse

Asquith, William H.

2014-01-01

The implementation characteristics of two method of L-moments (MLM) algorithms for parameter estimation of the 4-parameter Asymmetric Exponential Power (AEP4) distribution are studied using the R environment for statistical computing. The objective is to validate the algorithms for general application of the AEP4 using R. An algorithm was introduced in the original study of the L-moments for the AEP4. A second or alternative algorithm is shown to have a larger L-moment-parameter domain than the original. The alternative algorithm is shown to provide reliable parameter production and recovery of L-moments from fitted parameters. A proposal is made for AEP4 implementation in conjunction with the 4-parameter Kappa distribution to create a mixed-distribution framework encompassing the joint L-skew and L-kurtosis domains. The example application provides a demonstration of pertinent algorithms with L-moment statistics and two 4-parameter distributions (AEP4 and the Generalized Lambda) for MLM fitting to a modestly asymmetric and heavy-tailed dataset using R.

N =4 supergravity next-to-maximally-helicity-violating six-point one-loop amplitude

NASA Astrophysics Data System (ADS)

Dunbar, David C.; Perkins, Warren B.

2016-12-01

We construct the six-point, next-to-maximally-helicity-violating one-loop amplitude in N =4 supergravity using unitarity and recursion. The use of recursion requires the introduction of rational descendants of the cut-constructible pieces of the amplitude and the computation of the nonstandard factorization terms arising from the loop integrals.

Use of libration-point orbits for space observatories

NASA Technical Reports Server (NTRS)

Farquhar, Robert W.; Dunham, David W.

1990-01-01

The sun-earth libration points, L1 and L2, are located 1.5 million kilometers from the earth toward and away from the sun. Halo orbits about these points have significant advantages for space observatories in terms of viewing geometry, thermal and radiation environment, and delta-V expediture.

Early Mission Maneuver Operations for the Deep Space Climate Observatory Sun-Earth L1 Libration Point Mission

NASA Technical Reports Server (NTRS)

Roberts, Craig; Case, Sara; Reagoso, John; Webster, Cassandra

2015-01-01

The Deep Space Climate Observatory mission launched on February 11, 2015, and inserted onto a transfer trajectory toward a Lissajous orbit around the Sun-Earth L1 libration point. This paper presents an overview of the baseline transfer orbit and early mission maneuver operations leading up to the start of nominal science orbit operations. In particular, the analysis and performance of the spacecraft insertion, mid-course correction maneuvers, and the deep-space Lissajous orbit insertion maneuvers are discussed, com-paring the baseline orbit with actual mission results and highlighting mission and operations constraints..

Characterization of the TIP4P-Ew water model: vapor pressure and boiling point.

PubMed

Horn, Hans W; Swope, William C; Pitera, Jed W

2005-11-15

The liquid-vapor-phase equilibrium properties of the previously developed TIP4P-Ew water model have been studied using thermodynamic integration free-energy simulation techniques in the temperature range of 274-400 K. We stress that free-energy results from simulations need to be corrected in order to be compared to the experiment. This is due to the fact that the thermodynamic end states accessible through simulations correspond to fictitious substances (classical rigid liquids and classical rigid ideal gases) while experiments operate on real substances (liquids and real gases, with quantum effects). After applying analytical corrections the vapor pressure curve obtained from simulated free-energy changes is in excellent agreement with the experimental vapor pressure curve. The boiling point of TIP4P-Ew water under ambient pressure is found to be at 370.3+/-1.9 K, about 7 K higher than the boiling point of TIP4P water (363.7+/-5.1 K; from simulations that employ finite range treatment of electrostatic and Lennard-Jones interactions). This is in contrast to the approximately +15 K by which the temperature of the density maximum and the melting temperature of TIP4P-Ew are shifted relative to TIP4P, indicating that the temperature range over which the liquid phase of TIP4P-Ew is stable is narrower than that of TIP4P and resembles more that of real water. The quality of the vapor pressure results highlights the success of TIP4P-Ew in describing the energetic and entropic aspects of intermolecular interactions in liquid water.

Seeking a Human Spaceflight Program Worthy of a Great Nation. Review of U.S. Human Spaceflight Plans Committee

DTIC Science & Technology

2009-10-01

human spaceflight plan is its affordability. In the way of background , Fig- ures 1-4, 1- 5 and 1-6 present the overall NASA bud- get trend over time...build upon the experience gained from prior ones. All as- sume a first flight to lunar orbit, and then to the Lagrange points, and then to near-Earth...and a description of the scenarios considered for inclusion in the integrated options presented in this report. 4.1.1 Background . The Space

Soil Moisture Active Passive (SMAP) Mission Level 4 Surface and Root Zone Soil Moisture (L4_SM) Product Specification Document

NASA Technical Reports Server (NTRS)

Reichle, Rolf H.; Ardizzone, Joseph V.; Kim, Gi-Kong; Lucchesi, Robert A.; Smith, Edmond B.; Weiss, Barry H.

2015-01-01

This is the Product Specification Document (PSD) for Level 4 Surface and Root Zone Soil Moisture (L4_SM) data for the Science Data System (SDS) of the Soil Moisture Active Passive (SMAP) project. The L4_SM data product provides estimates of land surface conditions based on the assimilation of SMAP observations into a customized version of the NASA Goddard Earth Observing System, Version 5 (GEOS-5) land data assimilation system (LDAS). This document applies to any standard L4_SM data product generated by the SMAP Project. The Soil Moisture Active Passive (SMAP) mission will enhance the accuracy and the resolution of space-based measurements of terrestrial soil moisture and freeze-thaw state. SMAP data products will have a noteworthy impact on multiple relevant and current Earth Science endeavors. These include: Understanding of the processes that link the terrestrial water, the energy and the carbon cycles, Estimations of global water and energy fluxes over the land surfaces, Quantification of the net carbon flux in boreal landscapes Forecast skill of both weather and climate, Predictions and monitoring of natural disasters including floods, landslides and droughts, and Predictions of agricultural productivity. To provide these data, the SMAP mission will deploy a satellite observatory in a near polar, sun synchronous orbit. The observatory will house an L-band radiometer that operates at 1.40 GHz and an L-band radar that operates at 1.26 GHz. The instruments will share a rotating reflector antenna with a 6 meter aperture that scans over a 1000 km swath.

Morphogenetic effects of 2,4-dichlorophenoxyacetic acid on pinto bean (Phaseolus vulgaris L.) leaf explants in vitro.

PubMed

Saunders, J W; Hosfield, G L; Levi, A

1987-02-01

Roots, callus and/or globular structures were produced on primary leaf and distal cotyledon explants of pinto bean (Phaseolus vulgaris L. cv. UI 114) cultured on semisolid MS medium with a wide range of 2,4-D concentrations (0.01 to 80 mg/L) with either 0 or 1.0 mg/L kinetin. Explants rooted at lower 2,4-D concentrations than at those favoring globule formation on callus, although roots, callus and globules often developed from the same explant. Isolated opaque green globular structures developed when callus initiated on media with 3 or more mg/L 2,4-D was subcultured in liquid MS + 30 mg/L 2,4-D. These structures multiplied with a fresh weight doubling time of 8-9 days in MS + 30 mg/L 2,4-D. Although this multiplicative behavior and opaque color were reminiscent of embryoids reported for other species, no cotyledons or roots were seen.

Process optimization for enhancing production of cis-4-hydroxy-L-proline by engineered Escherichia coli.

PubMed

Chen, Kequan; Pang, Yang; Zhang, Bowen; Feng, Jiao; Xu, Sheng; Wang, Xin; Ouyang, Pingkai

2017-11-22

Understanding the bioprocess limitations is critical for the efficient design of biocatalysts to facilitate process feasibility and improve process economics. In this study, a proline hydroxylation process with recombinant Escherichia coli expressing L-proline cis-4-hydroxylase (SmP4H) was investigated. The factors that influencing the metabolism of microbial hosts and process economics were focused on for the optimization of cis-4-hydroxy-L-proline (CHOP) production. In recombinant E. coli, SmP4H synthesis limitation was observed. After the optimization of expression system, CHOP production was improved in accordance with the enhanced SmP4H synthesis. Furthermore, the effects of the regulation of proline uptake and metabolism on whole-cell catalytic activity were investigated. The improved CHOP production by repressing putA gene responsible for L-proline degradation or overexpressing L-proline transporter putP on CHOP production suggested the important role of substrate uptake and metabolism on the whole-cell biocatalyst efficiency. Through genetically modifying these factors, the biocatalyst activity was significantly improved, and CHOP production was increased by twofold. Meanwhile, to further improve process economics, a two-strain coupling whole-cell system was established to supply co-substrate (α-ketoglutarate, α-KG) with a cheaper chemical L-glutamate as a starting material, and 13.5 g/L of CHOP was successfully produced. In this study, SmP4H expression, and L-proline uptake and degradation, were uncovered as the hurdles for microbial production of CHOP. Accordingly, the whole-cell biocatalysts were metabolically engineered for enhancing CHOP production. Meanwhile, a two-strain biotransformation system for CHOP biosynthesis was developed aiming at supplying α-KG more economically. Our work provided valuable insights into the design of recombinant microorganism to improve the biotransformation efficiency that catalyzed by Fe

Advancements in the treatment of hypothyroidism with L-T4 liquid formulation or soft gel capsule: an update.

PubMed

Fallahi, Poupak; Ferrari, Silvia Martina; Ruffilli, Ilaria; Ragusa, Francesca; Biricotti, Marco; Materazzi, Gabriele; Miccoli, Paolo; Antonelli, Alessandro

2017-05-01

The most recent advance concerning levothyroxine (L-T4) therapy is the development of novel oral formulations: the liquid preparation, and the soft gel capsule. Areas covered: This review evaluates the most recent clinical studies about these new formulations. The liquid formulation has been shown to overcome: the food and beverages intereference with L-T4 tablets absorption, caused by food or coffee at breakfast; malabsorption induced by the increased gastric pH, resulting from atrophic gastritis, or due to proton-pump inhibitors; and malabsorption after bariatric surgery. The use of liquid L-T4 has been studied also in pregnancy, newborns and infants, suggesting a better bioequivalence than tablets. Finally, liquid L-T4 is more active than tablets in the control of thyroid-stimulating hormone (TSH) in hypothyroid patients without malabsorption, drug interference, or gastric disorders, leading to a hypothesized higher absorption of liquid L-T4 also in these patients. Few studies have evaluated soft gel L-T4 with promising results in patients with malabsorption related to coffee or gastritis. Expert opinion: Liquid L-T4 (and soft gel capsules) are more active than the tablet L-T4 in the control of TSH in hypothyroid patients with gastric disorders, malabsorption, or drug interference, but also in patients without absorption disorders.

Sidi Ali Ou Azza (L4): A New Moroccan Fall

NASA Astrophysics Data System (ADS)

Chennaoui Aoudjehane, H.; Agee, C. B.; Aaranson, A.; Bouragaa, A.

2016-08-01

Sidi Ali Ou Azza is the latest meteorite fall in Morocco, it occurred on 28 July 2015 very close (about 40 km) to Tissint martian shergottite fall that occurred on 18 July 2011. It's one of the small group of 23 L4 ordinary chondrite falls.

Stability of levothyroxine sodium 0.4 microg/mL in 0.9% sodium chloride injection.

PubMed

Stadalman, Kelli A; Kelner, Michael J; Box, Kevin; Dominguez, Alex; Rigby, Joseph F

2009-12-01

Intravenous levothyroxine therapy decreases vasopressor requirements and prevents cardiovascular collapse in hemodynamically unstable patients eligible for organ donation. The stability of levothyroxine when used in this manner is unknown. To determine the stability of levothyroxine solution for intravenous use at a concentration of 0.4 microg/mL diluted in 0.9% sodium chloride. Triplicate sample sets were prepared by reconstituting levothyroxine 200 microg for injection with 5 mL of 0.9% sodium chloride with further dilution in 500 mL of 0.9% sodium chloride. One sample set was protected from light and the other was left unprotected. Both sample sets were stored at room temperature, and samples from each were analyzed for initial concentration and 4, 8, 12, and 24 hours later. Levothyroxine sodium 0.4 microg/mL in 500 mL 0.9% sodium chloride is stable for 24 hours at room temperature when protected from light.

Isoelectric point and adsorption activity of porous g-C3N4

NASA Astrophysics Data System (ADS)

Zhu, Bicheng; Xia, Pengfei; Ho, Wingkei; Yu, Jiaguo

2015-07-01

The isoelectric point (IEP) is an important physicochemical parameter of many compounds, such as oxides, hydroxides, and nitrides, and can contribute to estimation of the surface charges of compound particles at various pH conditions. In this work, three types of graphitic carbon nitrides (g-C3N4) were synthesized by directly heating melamine, thiourea, and urea. The prepared samples showed different microstructures and IEPs that influenced their adsorption activity. Differences in microstructure resulted from the various precursors used during synthesis. The IEPs of the obtained g-C3N4 were measured to be approximately 4-5, which is due to the equilibrium of chemical reactions between hydrogen ions, hydroxyl ions, and amine groups on the g-C3N4 surface. The IEP of g-C3N4 prepared from thiourea was lower than those of the corresponding samples prepared from melamine and urea. The adsorption activity of methylene blue on g-C3N4 prepared from urea and thiourea was excellent, which indicates that g-C3N4 is a promising adsorbent. This work provides a useful reference for choosing precursors with which to prepare g-C3N4 and combining g-C3N4 with other compounds in solution.

An Unstable Arch Model of a Solar Flare

DTIC Science & Technology

1976-08-10

where the Euler-Lagrange equation becomes singular. We now expand f(r) and g(r) around the singular point r. Thus k JA + - p x, (3.20) m where x...current layer, etc.) can result only from convection. The equations we will use are ()B E 2 - VX(vXB)- _ VX(1TV XB), (4.8) E+ vX B=77j (4.9) C and curl (P...d- curl (J ( curl B) X B+pg . (4.10) Since the treatment is principally concerned with resistive instabilities whose growth times are long compared to

Effects of L-threo-3,4-dihydroxyphenylserine on orthostatic hypotension in hemodialysis patients.

PubMed

Iida, Nobutoshi; Koshikawa, Syozo; Akizawa, Tadao; Tsubakihara, Yoshiharu; Marumo, Fumiaki; Akiba, Takashi; Kawaguchi, Yoshindo; Imada, Akio; Yamazaki, Chikao; Suzuki, Masashi

2002-01-01

Orthostatic hypotension (OH) is a serious complication observed in hemodialysis (HD) patients after HD as well as during the interdialytic period. L-Threo-3,4-dihydroxyphenylserine (L-DOPS) is a nonphysiological neutral amino acid that is directly converted to the neurotransmitter norepinephrine by aromatic L-amino acid decarboxylase. A placebo-controlled double-blind study for 4 consecutive weeks and a long-term study (24-52 weeks) were conducted to evaluate the efficacy of L-DOPS for OH after HD. The drug was administered orally 30 min before the start of each HD period in both studies. Doses of 400 mg of L-DOPS or placebo were given to HD patients with OH (45 and 41 patients, respectively) in the double-blind study, and doses of 200 or 400 mg of L-DOPS were given to 74 HD patients in the long-term study. In the double-blind study, L-DOPS significantly ameliorated subjective symptoms related to OH, including dizziness/light-headed feeling, and malaise, throughout the interdialytic period. For 19 patients with delayed-type OH, hypotension with the lowest blood pressure recorded 10 min after standing, the decrease in blood pressure was suppressed significantly after L-DOPS treatment (10 patients) as compared with the placebo-treated group (9 patients). In the long-term study, the efficacy of L-DOPS was not attenuated, and the marked fluctuations in the plasma L-DOPS and norepinephrine levels were not noted after long-term use, without increases in incidence or severity of adverse reactions. These results indicate that L-DOPS is effective for improving OH-related interdialytic subjective symptoms in HD patients after short-term as well as after long-term administration. Copyright 2002 S. Karger AG, Basel

Monitoring solar irradiance from L2 with Gaia

NASA Astrophysics Data System (ADS)

Serpell, E.

2017-09-01

Gaia is the European Space Agency's cornerstone astrometry mission to measure the positions of a billion stars in the Milky Way with unprecedented accuracy. Since early 2014 Gaia has been operating in a halo orbit around the second Sun-Earth Lagrange point that provides the stable thermal environment, without Earth eclipses, needed for the payload to function accurately. The spacecraft is equipped with a number of thermally isolated, sun-facing thermistors that provide a continuous measurement of the local equilibrium temperature. As a consequence of the spacecraft design and operational conditions these temperature measurements have been used to infer the solar output over a broad wavelength range. In this paper we present an analysis of temperature measurements made of the Gaia solar panels at frequencies of up to 1 Hz for the first 35 months of routine operations. We show that the Gaia solar panel temperature measurements are capable of precisely determining short term changes to the solar output at a level of better than 0.04% with time constants of a few minutes.

Cooperative heteroassembly of the adenoviral L4-22K and IVa2 proteins onto the viral packaging sequence DNA.

PubMed

Yang, Teng-Chieh; Maluf, Nasib Karl

2012-02-21

Human adenovirus (Ad) is an icosahedral, double-stranded DNA virus. Viral DNA packaging refers to the process whereby the viral genome becomes encapsulated by the viral particle. In Ad, activation of the DNA packaging reaction requires at least three viral components: the IVa2 and L4-22K proteins and a section of DNA within the viral genome, called the packaging sequence. Previous studies have shown that the IVa2 and L4-22K proteins specifically bind to conserved elements within the packaging sequence and that these interactions are absolutely required for the observation of DNA packaging. However, the equilibrium mechanism for assembly of IVa2 and L4-22K onto the packaging sequence has not been determined. Here we characterize the assembly of the IVa2 and L4-22K proteins onto truncated packaging sequence DNA by analytical sedimentation velocity and equilibrium methods. At limiting concentrations of L4-22K, we observe a species with two IVa2 monomers and one L4-22K monomer bound to the DNA. In this species, the L4-22K monomer is promoting positive cooperative interactions between the two bound IVa2 monomers. As L4-22K levels are increased, we observe a species with one IVa2 monomer and three L4-22K monomers bound to the DNA. To explain this result, we propose a model in which L4-22K self-assembly on the DNA competes with IVa2 for positive heterocooperative interactions, destabilizing binding of the second IVa2 monomer. Thus, we propose that L4-22K levels control the extent of cooperativity observed between adjacently bound IVa2 monomers. We have also determined the hydrodynamic properties of all observed stoichiometric species; we observe that species with three L4-22K monomers bound have more extended conformations than species with a single L4-22K bound. We suggest this might reflect a molecular switch that controls insertion of the viral DNA into the capsid.

Chiropractic and rehabilitation management of a patient with extraforaminal entrapment of L4 nerve with balance problem.

PubMed

Alagha, Babak

2015-01-01

Disc herniation is one of the most common causes of low back pain with radicular pain. Among various types of disc herniation, the extraforaminal disc herniation is a rare cause of lumbar radiculopathy. The aim of presenting this case study is to demonstrate the benefits of Chiropractic care including spine and extremity manipulation and rehabilitation in the treatment of a rare case of extraforaminal L4 nerve entrapment causing severe L4 radiculopathy and chronic mild low back pain (LBP). The aim of presenting this case study is to demonstrate the benefits of Chiropractic care including spine and extremity manipulation and rehabilitation in treatment of rare case of extraforaminal L4 nerve entrapment which caused severe L4 radiculopathy and chronic mild low back pain (LBP). A 45-year old female patient arrived at the clinic with chronic mild low back pain and right buttock pain, all of which had presented for two years' duration. During the preceding month, the radicular pain initiated in medium to high intensity, radiating to her right leg following the L4 dermatomal pattern with a periodic tingling sensation in her right foot. A neuro exam demonstrated a proprioception deficit in her right leg. A Romberg test was positive. The patient was treated by low amplitude high velocity spinal and extremity manipulation for 10 consecutive sessions (2 weeks), followed by rehabilitation and exercise therapy including advanced myofascial release therapy for an additional 12 sessions (4 weeks). After treatment, the patient reported a significant improvement in her low back pain and radiculopathy. In addition, she achieved some improvement in balance. It seems that Chiropractic care and rehabilitation therapy may be a safe and effective modality in treatment of an L4 radiculopathy in a patient with an extraforaminal L4 nerve entrapment. Although it is rare, an L4 extraforaminal disc herniation should be considered as a possible cause of symptoms in patients with chronic mild

A Free-Return Earth-Moon Cycler Orbit for an Interplanetary Cruise Ship

NASA Technical Reports Server (NTRS)

Genova, Anthony L.; Aldrin, Buzz

2015-01-01

A periodic circumlunar orbit is presented that can be used by an interplanetary cruise ship for regular travel between Earth and the Moon. This Earth-Moon cycler orbit was revealed by introducing solar gravity and modest phasing maneuvers (average of 39 m/s per month) which yields close-Earth encounters every 7 or 10 days. Lunar encounters occur every 26 days and offer the chance for a smaller craft to depart the cycler and enter lunar orbit, or head for a Lagrange point (e.g., EM-L2 halo orbit), distant retrograde orbit (DRO), or interplanetary destination such as a near-Earth object (NEO) or Mars. Additionally, return-to-Earth abort options are available from many points along the cycling trajectory.

33. Photocopy of drawing STANDARD PLAN L4, SHEET 6 OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

33. Photocopy of drawing STANDARD PLAN L-4, SHEET 6 OF 8; CROSS SECTION, FRONT ELEVATION WITH SHUTTERS, FIRE FINDER - Suntop Lookout, Forest Road 510, Mt. Baker-Snoqualmie National Forest, Greenwater, Pierce County, WA

Shot noise in systems with semi-Dirac points

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zhai, Feng; Wang, Juan

2014-08-14

We calculate the ballistic conductance and shot noise of electrons through a two-dimensional stripe system (width W ≫ length L) with semi-Dirac band-touching points. We find that the ratio between zero-temperature noise power and mean current (the Fano factor) is highly anisotropic. When the transport is along the linear-dispersion direction and the Fermi energy is fixed at the semi-Dirac point, the Fano factor has a universal value F = 0.179 while a minimum conductivity exists and scales with L{sup 1∕2}. Along the parabolic dispersion direction, the Fano factor at the semi-Dirac point has a contact-independent limit exceeding 0.9, which varies weakly withmore » L due to the common-path interference of evanescent waves. Our findings suggest a way to discern the type of band-touching points.« less

[Biomechanical analysis on the correlation between iliac rotation displacement and L(4,5) disc degeneration].

PubMed

Shi, Ning-ning; Shen, Guo-quan; He, Shui-yong; Guo, Ru-bao

2016-05-01

To study the biomechanical relationship between iliac rotation displacement and L(4,5) disc degeneration, and to provide clinical evidences for the prevention and treatment of L(4,5) disc degeneration and herniation. From March 2012 to February 2014,68 patients with lumbar disc herniation combined with sacroiliac joint disorders were selected. Among them, 42 patients with L(4,5) disc herniation combined with sacroiliac joint disorders included 22 males and 20 females, ranging in age from 19 to 63 years old, with an average of (51.78 +/- 20.18) years old, and the duration of the disease ranged from 1 to 126 months with an average of (11.18 +/- 9.23) months. Twenty-six patients with L5S1 disc herniation combined with sacroiliac joint disorders included 11 males and 15 females, ranging in age from18 to 65 years old with an average of (45.53 +/- 27.23) years old, and the duration of the disease ranged from 0.5 to 103 months with an average of (11.99 +/- 12.56) months. Sixty-eight anteroposterior lumbar radiographs, 68 lateral lumbar radiographs,and 68 pelvic plain films were taken. The degree of lumbar scoliosis, pelvic tilt,and disc thickness were measured. The correlation between pelvic tilt and lumbar scoliosis ,lumbar scoliosis and disc thickness were studied by using linear and regression methods. The hiomechanical analysis was performed. There was a positive correlation between pelvic tilt and lumbar scoliosis in patients with L(4,5) disk herniation (R=0.49, P=0.00). There was a causal relationship and good linear proportional relationship (Y=3.05+1.07X, P=0.00) in the two variables. There was a negative correlation between lumbar scoliosis and intervertebral space in male patients with L (4,5) disk herniation (R = -0.50, P=0.01). There was a causal relationship and good linear proportional relationship in the two variables (Y=13.09-0.27X, P=0.02). But there was a positive correlation between lumbar scoliosis and intervertebral space in male patients with L5S1

Expression of Genes Encoding the Enzymes for Glycogen and Trehalose Metabolism in L3 and L4 Larvae of Anisakis simplex.

PubMed

Łopieńska-Biernat, E; Zaobidna, E A; Dmitryjuk, M

2015-01-01

Trehalose and glycogen metabolism plays an important role in supporting life processes in many nematodes, including Anisakis simplex. Nematodes, cosmopolitan helminths parasitizing sea mammals and humans, cause a disease known as anisakiasis. The aim of this study was to investigate the expression of genes encoding the enzymes involved in the metabolism of trehalose and glycogen-trehalose-6-phosphate synthase (TPS), trehalose-6-phosphate phosphatase (TPP), glycogen synthase (GS), and glycogen phosphorylase (GP)-in stage L3 and stage L4 larvae of A. simplex. The expression of mRNA all four genes, tps, tpp, gs, and gp, was examined by real-time polymerase chain reaction. The A. simplex ribosomal gene (18S) was used as a reference gene. Enzymatic activity was determined. The expression of trehalose enzyme genes was higher in L3 than in L4 larvae, but an inverse relationship was noted for the expression of gs and gp genes.

Expression of Genes Encoding the Enzymes for Glycogen and Trehalose Metabolism in L3 and L4 Larvae of Anisakis simplex

PubMed Central

Łopieńska-Biernat, E.; Zaobidna, E. A.; Dmitryjuk, M.

2015-01-01

Trehalose and glycogen metabolism plays an important role in supporting life processes in many nematodes, including Anisakis simplex. Nematodes, cosmopolitan helminths parasitizing sea mammals and humans, cause a disease known as anisakiasis. The aim of this study was to investigate the expression of genes encoding the enzymes involved in the metabolism of trehalose and glycogen—trehalose-6-phosphate synthase (TPS), trehalose-6-phosphate phosphatase (TPP), glycogen synthase (GS), and glycogen phosphorylase (GP)—in stage L3 and stage L4 larvae of A. simplex. The expression of mRNA all four genes, tps, tpp, gs, and gp, was examined by real-time polymerase chain reaction. The A. simplex ribosomal gene (18S) was used as a reference gene. Enzymatic activity was determined. The expression of trehalose enzyme genes was higher in L3 than in L4 larvae, but an inverse relationship was noted for the expression of gs and gp genes. PMID:26783451

Isolation and Synthetic Diversification of Jadomycin 4-Amino-l-phenylalanine.

PubMed

Martinez-Farina, Camilo F; Robertson, Andrew W; Yin, Huimin; Monro, Susan; McFarland, Sherri A; Syvitski, Raymond T; Jakeman, David L

2015-06-26

Streptomyces venezuelae ISP5230 was grown in the presence of phenylalanine analogues to observe whether they could be incorporated into novel jadomycin structures. It was found that the bacteria successfully produced jadomycins incorporating 4-aminophenylalanine enantiomers. Upon isolation and characterization of jadomycin 4-amino-l-phenylalanine (1), it was synthetically derivatized, using activated succinimidyl esters, to yield a small jadomycin amide library. These are the first examples of oxazolone-ring-containing jadomycins that have incorporated an amino functionality subsequently used for derivatization.

Power transmission by laser beam from lunar-synchronous satellite

NASA Technical Reports Server (NTRS)

Williams, M. D.; Deyoung, R. J.; Schuster, G. L.; Choi, S. H.; Dagle, J. E.; Coomes, E. P.; Antoniak, Z. I.; Bamberger, J. A.; Bates, J. M.; Chiu, M. A.

1993-01-01

The possibility of beaming power from synchronous lunar orbits (the L1 and L2 Lagrange points) to a manned long-range lunar rover is addressed. The rover and two versions of a satellite system (one powered by a nuclear reactor, the other by photovoltaics) are described in terms of their masses, geometries, power needs, missions, and technological capabilities. Laser beam power is generated by a laser diode array in the satellite and converted to 30 kW of electrical power at the rover. Present technological capabilities, with some extrapolation to near future capabilities, are used in the descriptions. The advantages of the two satellite/rover systems over other such systems and over rovers with onboard power are discussed along with the possibility of enabling other missions.

The MALATANG Survey: The L GAS–L IR Correlation on Sub-kiloparsec Scale in Six Nearby Star-forming Galaxies as Traced by HCN J = 4 → 3 and HCO+ J = 4 → 3

NASA Astrophysics Data System (ADS)

Tan, Qing-Hua; Gao, Yu; Zhang, Zhi-Yu; Greve, Thomas R.; Jiang, Xue-Jian; Wilson, Christine D.; Yang, Chen-Tao; Bemis, Ashley; Chung, Aeree; Matsushita, Satoki; Shi, Yong; Ao, Yi-Ping; Brinks, Elias; Currie, Malcolm J.; Davis, Timothy A.; de Grijs, Richard; Ho, Luis C.; Imanishi, Masatoshi; Kohno, Kotaro; Lee, Bumhyun; Parsons, Harriet; Rawlings, Mark G.; Rigopoulou, Dimitra; Rosolowsky, Erik; Bulger, Joanna; Chen, Hao; Chapman, Scott C.; Eden, David; Gear, Walter K.; Gu, Qiu-Sheng; He, Jin-Hua; Jiao, Qian; Liu, Dai-Zhong; Liu, Li-Jie; Li, Xiao-Hu; Michałowski, Michał J.; Nguyen-Luong, Quang; Qiu, Jian-Jie; Smith, Matthew W. L.; Violino, Giulio; Wang, Jian-Fa; Wang, Jun-Feng; Wang, Jun-Zhi; Yeh, Sherry; Zhao, Ying-He; Zhu, Ming

2018-06-01

We present {HCN} J=4\\to 3 and {HCO}}+ J=4\\to 3 maps of six nearby star-forming galaxies, NGC 253, NGC 1068, IC 342, M82, M83, and NGC 6946, obtained with the James Clerk Maxwell Telescope as part of the MALATANG survey. All galaxies were mapped in the central 2‧ × 2‧ region at 14″ (FWHM) resolution (corresponding to linear scales of ∼0.2–1.0 kpc). The L IR–L‧dense relation, where the dense gas is traced by the {HCN} J=4\\to 3 and the {HCO}}+ J=4\\to 3 emission, measured in our sample of spatially resolved galaxies is found to follow the linear correlation established globally in galaxies within the scatter. We find that the luminosity ratio, L IR/L‧dense, shows systematic variations with L IR within individual spatially resolved galaxies, whereas the galaxy-integrated ratios vary little. A rising trend is also found between L IR/L‧dense ratio and the warm-dust temperature gauged by the 70 μm/100 μm flux ratio. We find that the luminosity ratios of IR/HCN (4–3) and IR/HCO+ (4–3), which can be taken as a proxy for the star formation efficiency (SFE) in the dense molecular gas (SFEdense), appear to be nearly independent of the dense gas fraction (f dense) for our sample of galaxies. The SFE of the total molecular gas (SFEmol) is found to increase substantially with f dense when combining our data with those on local (ultra)luminous infrared galaxies and high-z quasars. The mean L{{\\prime} }HCN(4{--}3)}/L{{\\prime} }HCO}+(4{--}3)} line ratio measured for the six targeted galaxies is 0.9 ± 0.6. No significant correlation is found for the L{{\\prime} }HCN(4{--}3)}/L{{\\prime} }HCO}+(4{--}3)} ratio with the star formation rate as traced by L IR, nor with the warm-dust temperature, for the different populations of galaxies.

Intrinsic point defects in off-stoichiometric Cu2ZnSnSe4: A neutron diffraction study

NASA Astrophysics Data System (ADS)

Gurieva, Galina; Valle Rios, Laura Elisa; Franz, Alexandra; Whitfield, Pamela; Schorr, Susan

2018-04-01

This work is an experimental study of intrinsic point defects in off-stoichiometric kesterite type CZTSe by means of neutron powder diffraction. We revealed the existence of copper vacancies (VCu), various cation anti site defects (CuZn, ZnCu, ZnSn, SnZn, and CuZn), as well as interstitials (Cui, Zni) in a wide range of off-stoichiometric polycrystalline powder samples synthesized by the solid state reaction. The results show that the point defects present in off-stoichiometric CZTSe agree with the off-stoichiometry type model, assuming certain cation substitutions accounting for charge balance. In addition to the known off-stoichiometry types A-H, new types (I-L) have been introduced. For the very first time, a correlation between the chemical composition of the CZTSe kesterite type phase and the occurring intrinsic point defects is presented. In addition to the off-stoichiometry type specific defects, the Cu/Zn disorder is always present in the CZTSe phase. In Cu-poor/Zn-rich CZTSe, a composition considered as the one that delivers the best photovoltaic performance, mainly copper vacancies, ZnCu and ZnSn anti sites are present. Also, this compositional region shows the lowest degree of Cu/Zn disorder.

Prevention of suture knot exposure in posterior chamber intraocular lens implantation by 4-point scleral fixation technique.

PubMed

Baykara, Mehmet; Avci, Remzi

2004-01-01

The results and complications of posterior chamber intraocular lens (IOL) implantation by a 4-point scleral fixation technique are described. Fifty eyes of 47 patients who underwent scleral-fixated IOL implantation were retrospectively evaluated. Twenty-one (42%) eyes had a history of trauma and 29 (58%) eyes had previously undergone cataract surgery. In all cases, IOL implantation by 4-point scleral fixation was performed and the knots of fixation sutures were rotated and buried in the globe. The IOL position was adjusted by suture rotation for best centration. The mean follow-up time was 7 +/- 4 months. Four (8%) eyes had minimal corneal edema preoperatively. Cystoid macular edema was noted in 2 (6.8%) eyes in the cataract surgery group and 8 (38%) eyes in the posttraumatic group. Two (9.5%) eyes in the posttraumatic group had atrophic macular changes and 1 (4.7%) had corneal scarring, which impaired vision. No complications such as knot exposure, tilting of the IOL, decentralization, or endophthalmitis were noted postoperatively. Postoperative mean corrected visual acuity was 0.4 +/- 0.3 in the posttraumatic group and 0.4 +/- 0.2 in the cataract surgery group. The 4-point scleral fixation technique resulted in no serious postoperative complications such as suture exposure and endophthalmitis. Because the knot can be rotated and buried in the globe, knot exposure is less likely to occur. This procedure is more effective than other techniques regarding IOL centralization.

Biosensor for the enantioselective analysis of the thyroid hormones (+)-3,3',5-triiodo-L-thyronine (T3) and (+)-3,3',5,5'-tetraiodo-L-thyronine (T4).

PubMed

Aboul-Enein, Hassan V; Stefan, Raluca-Ioana; Litescu, Simona; Radu, Gabriel Lucian

2002-01-01

An amperometric biosensor based on L-aminoacid oxidase is proposed for enantioselective assay of (+)-3,3',5-triiodo-L-thyronine (L-T3) and (+)-3,3',5,5'-tetraiodo-L-thyronine (L-T4), due to the fact that only the L enantiomer has the hormonal activity. The construction of the amperometric biosensor is simple and reproducible. The analytical information obtained from enantioselective analysis are reliable. The RSD <1% assured by using the amperometric biosensors for L enantiomers assay as raw materials, and from tablets, demonstrated their suitability for the analysis of T3 and T4 at ppb concentration levels.

International Space Station Centrifuge Rotor Models A Comparison of the Euler-Lagrange and the Bond Graph Modeling Approach

NASA Technical Reports Server (NTRS)

Nguyen, Louis H.; Ramakrishnan, Jayant; Granda, Jose J.

2006-01-01

The assembly and operation of the International Space Station (ISS) require extensive testing and engineering analysis to verify that the Space Station system of systems would work together without any adverse interactions. Since the dynamic behavior of an entire Space Station cannot be tested on earth, math models of the Space Station structures and mechanical systems have to be built and integrated in computer simulations and analysis tools to analyze and predict what will happen in space. The ISS Centrifuge Rotor (CR) is one of many mechanical systems that need to be modeled and analyzed to verify the ISS integrated system performance on-orbit. This study investigates using Bond Graph modeling techniques as quick and simplified ways to generate models of the ISS Centrifuge Rotor. This paper outlines the steps used to generate simple and more complex models of the CR using Bond Graph Computer Aided Modeling Program with Graphical Input (CAMP-G). Comparisons of the Bond Graph CR models with those derived from Euler-Lagrange equations in MATLAB and those developed using multibody dynamic simulation at the National Aeronautics and Space Administration (NASA) Johnson Space Center (JSC) are presented to demonstrate the usefulness of the Bond Graph modeling approach for aeronautics and space applications.

Diffuse interface simulation of bubble rising process: a comparison of adaptive mesh refinement and arbitrary lagrange-euler methods

NASA Astrophysics Data System (ADS)

Wang, Ye; Cai, Jiejin; Li, Qiong; Yin, Huaqiang; Yang, Xingtuan

2018-06-01

Gas-liquid two phase flow exists in several industrial processes and light-water reactors (LWRs). A diffuse interface based finite element method with two different mesh generation methods namely, the Adaptive Mesh Refinement (AMR) and the Arbitrary Lagrange Euler (ALE) methods is used to model the shape and velocity changes in a rising bubble. Moreover, the calculating speed and mesh generation strategies of AMR and ALE are contrasted. The simulation results agree with the Bhagat's experiments, indicating that both mesh generation methods can simulate the characteristics of bubble accurately. We concluded that: the small bubble rises as elliptical with oscillation, whereas a larger bubble (11 mm > d > 7 mm) rises with a morphology between the elliptical and cap type with a larger oscillation. When the bubble is large (d > 11 mm), it rises up as a cap type, and the amplitude becomes smaller. Moreover, it takes longer to achieve the stable shape from the ellipsoid to the spherical cap type with the increase of the bubble diameter. The results also show that for smaller diameter case, the ALE method uses fewer grids and has a faster calculation speed, but the AMR method can solve the case of a large geometry deformation efficiently.

Point Source X-Ray Lithography System for Sub-0.15 Micron Design Rules

DTIC Science & Technology

1998-05-22

consist of a SAL developed stepper, an SRL developed Dense Plasma Focus , (DPF), X-Ray source, and a CXrL developed beam line. The system will be...existing machine that used spark gap switching, SRL has developed an all solid state driver and improved head electrode assembly for their dense plasma ... focus X-Ray source. Likewise, SAL has used their existing Model 4 stepper installed at CXrL as a design starting point, and has developed an advanced

CXCL4L1 inhibits angiogenesis and induces undirected endothelial cell migration without affecting endothelial cell proliferation and monocyte recruitment.

PubMed

Sarabi, A; Kramp, B K; Drechsler, M; Hackeng, T M; Soehnlein, O; Weber, C; Koenen, R R; Von Hundelshausen, P

2011-01-01

The non-allelic variant of CXCL4/PF4, CXCL4L1/PF4alt, differs from CXCL4 in three amino acids of the C-terminal α-helix and has been characterized as a potent anti-angiogenic regulator. Although CXCL4 structurally belongs to the chemokine family, it does not behave like a 'classical' chemokine, lacking significant chemotactic properties. Specific hallmarks are its angiostatic, anti-proliferative activities, and proinflammatory functions, which can be conferred by heteromer-formation with CCL5/RANTES enhancing monocyte recruitment. Here we show that tube formation of endothelial cells was inhibited by CXCL4L1 and CXCL4, while only CXCL4L1 triggered chemokinesis of endothelial cells. The chemotactic response towards VEGF and bFGF was attenuated by both variants and CXCL4L1-induced chemokinesis was blocked by bFGF or VEGF. Endothelial cell proliferation was inhibited by CXCL4 (IC(50) 6.9 μg mL(-1)) but not by CXCL4L1, while both chemokines bound directly to VEGF and bFGF. Moreover, CXCL4 enhanced CCL5-induced monocyte arrest in flow adhesion experiments and monocyte recruitment into the mouse peritoneal cavity in vivo, whereas CXCL4L1 had no effect. CXCL4L1 revealed lower affinity to CCL5 than CXCL4, as quantified by isothermal fluorescence titration. As evidenced by the reduction of the activated partial thromboplastin time, CXCL4L1 showed a tendency towards less heparin-neutralizing activity than CXCL4 (IC(50) 2.45 vs 0.98 μg mL(-1)).  CXCL4L1 may act angiostatically by causing random endothelial cell locomotion, disturbing directed migration towards angiogenic chemokines, serving as a homeostatic chemokine with a moderate structural distinction yet different functional profile from CXCL4. © 2010 International Society on Thrombosis and Haemostasis.

L-threo-3,4-dihydroxyphenylserine (L-DOPS) co-administered with entacapone improves freezing of gait in Parkinson's disease.

PubMed

Fukada, Kei; Endo, Takuyuki; Yokoe, Masaru; Hamasaki, Toshimitsu; Hazama, Takanori; Sakoda, Saburo

2013-02-01

Parkinson's disease (PD) is a neurodegenerative disorder characterized by a variety of motor symptoms including freezing of gait (FOG), in which walking is transiently halted as if the patient's feet were 'glued to the ground'. Treatment of FOG is still challenging. Although L-threo-3,4-dihydroxyphenylserine (L-DOPS), a precursor of noradrenaline, has been on the market in Japan because of its beneficial effect for FOG, clinical use of L-DOPS has been far from satisfying. However, the fact that there were some responders to L-DOPS encouraged us to hypothesize that the enhancement of L-DOPS concentration in the brain by the co-administration of L-DOPS and a catechol-O-methyl transferase (COMT) inhibitor, which is expected to interrupt L-DOPS metabolism in the peripheral circulation, would be beneficial for FOG. Based on our hypothesis, we conducted a preliminary study with a small number of participants with FOG. Of the 16 PD patients with FOG who completed this study, group 1 (n=6) received L-DOPS co-administered with entacapone, which is a COMT inhibitor used worldwide as an anti-parkinson drug, group 2 (n=5) received entacapone alone, and group 3 (n=5) received L-DOPS alone. Only the patients in group 1 showed a significant improvement in FOG. Moreover, the beneficial effect was observed only in patients with levodopa-resistant FOG. This result supports our hypothesis, at least in patients with levodopa-resistant FOG, and shows that the co-administration of L-DOPS and entacapone could be a new strategy for FOG treatment. Copyright © 2012 Elsevier Ltd. All rights reserved.

Preliminary Design Considerations for Access and Operations in Earth-Moon L1/L2 Orbits

NASA Technical Reports Server (NTRS)

Folta, David C.; Pavlak, Thomas A.; Haapala, Amanda F.; Howell, Kathleen C.

2013-01-01

Within the context of manned spaceflight activities, Earth-Moon libration point orbits could support lunar surface operations and serve as staging areas for future missions to near-Earth asteroids and Mars. This investigation examines preliminary design considerations including Earth-Moon L1/L2 libration point orbit selection, transfers, and stationkeeping costs associated with maintaining a spacecraft in the vicinity of L1 or L2 for a specified duration. Existing tools in multi-body trajectory design, dynamical systems theory, and orbit maintenance are leveraged in this analysis to explore end-to-end concepts for manned missions to Earth-Moon libration points.

Pivots for pointing: visually-monitored pointing has higher arm elevations than pointing blindfolded.

PubMed

Wnuczko, Marta; Kennedy, John M

2011-10-01

Observers pointing to a target viewed directly may elevate their fingertip close to the line of sight. However, pointing blindfolded, after viewing the target, they may pivot lower, from the shoulder, aligning the arm with the target as if reaching to the target. Indeed, in Experiment 1 participants elevated their arms more in visually monitored than blindfolded pointing. In Experiment 2, pointing to a visible target they elevated a short pointer more than a long one, raising its tip to the line of sight. In Experiment 3, the Experimenter aligned the participant's arm with the target. Participants judged they were pointing below a visually monitored target. In Experiment 4, participants viewing another person pointing, eyes-open or eyes-closed, judged the target was aligned with the pointing arm. In Experiment 5, participants viewed their arm and the target via a mirror and posed their arm so that it was aligned with the target. Arm elevation was higher in pointing directly.